LOCUS       DASVES010000001         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1359605, whole genome shotgun sequence.
ACCESSION   DASVES010000001 DASVES010000000
VERSION     DASVES010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359605"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /gene="infC"
                     /locus_tag="VF240_00005"
     CDS             complement(<1..371)
                     /gene="infC"
                     /locus_tag="VF240_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX7173649.1"
                     /translation="MPEVRVIADDGEQLGVMNTRDAVRMAREKGLDLVEVAATADPPV
                     CRIIDFGKFQYEAKKKANEAKKKQVTITVKEVKFRPGTDDHDYNFKMKHAHQWLDEGD
                     KVKATIFFRGREITHRELGAKL"
     gene            complement(546..>2414)
                     /gene="thrS"
                     /locus_tag="VF240_00010"
     CDS             complement(546..>2414)
                     /gene="thrS"
                     /locus_tag="VF240_00010"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7173650.1"
                     /translation="ARVNGREVDLAYQLERPEGNGGGAASVEIEPVLPDTLDGLDVIR
                     HSTAHLLAAAVLDLFPGTKLGIGPALLDDPRYGFFYDLIAPRTLTDEDLPVIEKRMRD
                     LAKRNLAYRREELPKHEALRVFGERDEPLKCELVEDKGGDHVSVYYIDGSPFLDFCLG
                     PHVPNTNRLRAFKLLSLSGAYWMGDAEREQMQRIYGTAFATQDELDAWVKQREEAERR
                     DHRRLGRELDLFSINDQYGQGLIFWHPKGALIRKAMEDYLHAELIRRDYMFVVTPHIA
                     KRELWQISGHEQNYGDSMFSPMKIEESEFRLKPMNCPFHIAIYRSQQRSYRDLPQRYA
                     EMGTVYRAELSGAIHGLMRARGFTVDDAHLFCTPEQMGDEIRGCVEFAQDVFRTFGFE
                     KMKIELSVKGGDSAKTFLGTDEQWAAAEATLVAALEECGISYERMEGEGAFYGPKIDI
                     KIEDAIGRLWQLGTVQYDFNLPERFELEYIGEDNKAHRPMMVHRALFGSVERFFGVLI
                     EHYAGAFPLWLAPVQVAVLPITDRVNEYAAGVARTLREAGLRVEANLRSDKIGAKIRD
                     AQLQKVPFMLVLGDREVEQGQVAVRERARGDIGAMSLDEFKAMALRLVESRALTNT"
BASE COUNT          408 a          774 c          768 g          464 t
ORIGIN      
        1 agtttcgcgc ccagctcgcg gtgcgtaatc tcgcgcccgc ggaagaagat ggtggctttg
       61 accttgtcgc cctcgtcgag ccactggtgc gcgtgcttca tcttgaagtt gtagtcgtga
      121 tcgtccgtgc cggggcgaaa cttcacctcc ttgacggtga tcgtgacctg cttcttcttg
      181 gcctcgttgg ccttcttctt cgcctcgtac tggaacttgc cgaagtcgat gatgcggcac
      241 acgggcgggt cggccgtggc cgcgacctcg accagatcga ggcccttctc gcgggccatc
      301 ctcacggcgt cgcgcgtgtt catcacgccg agctgttcgc cgtcgtcggc gatcacgcgc
      361 acctcgggca cgcgaatcct ttcattgatg cgcgccgacg gcgtgcgccg gttgtcgaat
      421 cgctggccgc gtccacgaaa tcctgctatt gcaacacctc ctcagtcaga agttcaaagt
      481 tccgagttca aagttcaaag ggagacatgt ttccaacttt gaactttgaa ctttgaacct
      541 tgaacttatg tattcgtcag ggcgcgcgac tcgacgagtc ggagcgccat cgctttgaat
      601 tcatcgagcg acatcgcgcc gatgtcgccc cgcgcgcgct cgcgcacggc cacctgcccc
      661 tgctccacct cgcggtcgcc gaggacgagc ataaagggca ccttctgcaa ctgcgcgtcg
      721 cgaatcttcg cgccgatctt gtcactacgc agattcgcct cgacgcgcag acccgcctcg
      781 cgcaacgtgc gcgccacccc tgcggcgtac tcgttgacgc ggtcggtgat cgggagcacc
      841 gccacctgca cgggcgcgag ccaaagggga aacgcccccg cgtagtgctc gatgaggacg
      901 ccaaagaagc gttcgaccga gccgaagaga gcgcggtgaa ccatcatcgg ccggtgcgct
      961 ttattgtcct cgccgatgta ctcaagctcg aaacgctcgg gcaggttgaa gtcgtactgc
     1021 accgtcccca actgccagag gcggccgatg gcgtcctcga tcttgatgtc gatcttcggg
     1081 ccgtagaagg cgccctcgcc ctccatgcgc tcgtaactga tgccgcactc ttcgagcgcc
     1141 gcgacgagcg tcgcctcagc cgcggcccac tgctcgtccg tgccgaggaa ggtcttcgcg
     1201 gagtcgccgc ccttgacgga gagttcgatc ttcatcttct cgaagccgaa ggtgcggaac
     1261 acgtcctgcg cgaactcgac gcacccacgt atctcgtcgc ccatctgctc gggcgtgcag
     1321 aacagatgcg cgtcgtcaac ggtgaagccg cgggcgcgca tcaggccgtg gatcgcgccg
     1381 gagagttcgg cgcggtagac cgtccccatc tcggcgtagc gttgcggcag gtcgcgatag
     1441 ctgcgctgct gcgagcggta gatggcgatg tgaaacggac agttcatcgg cttgagccga
     1501 aactccgact cctcgatctt catgggcgag aacatcgagt cgccgtagtt ctgctcgtgg
     1561 ccgctgatct gccacaactc gcgcttggcg atgtgcggcg tgacgacgaa catgtaatcg
     1621 cgccgaatca actcggcgtg cagataatcc tccatcgcct tgcggatgag cgcgcccttc
     1681 gggtgccaga agatgaggcc ctgaccgtac tggtcgttga tggagaacag gtcaagctcg
     1741 cggcccagcc ggcggtggtc gcgacgctcg gcctcctcgc gttgtttgac ccacgcgtcg
     1801 agttcatcct gcgtggcgaa ggcggtgccg tagatgcgct gcatctgctc gcgctcggcg
     1861 tcgcccatcc agtaagcgcc cgagagcgag agcagcttga atgcgcgcag gcgattcgtg
     1921 ttcgggacgt gcgggccgag acagaagtcg aggaacggcg agccgtcgat gtaatagacg
     1981 ctcacatggt cgccgccctt gtcttcgacc agctcacact tcaaaggctc gtcgcgctcg
     2041 ccgaagacgc gcagggcctc gtgcttcggc agctcctctc gccggtaggc gaggttgcgc
     2101 ttggcgaggt cgcgcatgcg cttttcgatg acgggaaggt cttcatcggt gagcgtgcgc
     2161 ggggcgatca ggtcgtagaa aaagccgtag cgcgggtcgt ctaagagcgc cgggccgatg
     2221 ccgagcttgg tgccggggaa gaggtcgagg acggccgccg ccagaagatg cgccgtcgag
     2281 tgacggatga catcgaggcc gtcgagcgtg tccggcagca ccggctcgat ctcgacggaa
     2341 gcggcgccgc ccccgttccc ctccgggcgt tcgagttgat aggcgaggtc aacctcgcgg
     2401 ccgttaacgc gcgc
//
LOCUS       DASVES010000002        12063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_772530, whole genome shotgun sequence.
ACCESSION   DASVES010000002 DASVES010000000
VERSION     DASVES010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772530"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VF240_00015"
     CDS             complement(<1..1127)
                     /locus_tag="VF240_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX7173651.1"
                     /translation="MSRTVRTLLLSACLFGFALPPTASTQTQSPSQTPAAYVCPMHPA
                     VTSHAPGSCPQCKMKLVAKAAAKSAPQSETGPPSNWASLSAAERARALELLAPTYEHT
                     CPMHPEVRQAQEGVCPKCGMPLRSLNPSVRGNYRLDMTATPARPKANERVRLRFVVTH
                     PQTAAPVKSFVLNHERLFHLFVISRDLREYQHIHPQLDADGSFTVETALPREGSYKLH
                     SDFFPAGGTPQLVHQELATANHRSTPRHVAPAAVILTPDTTLTKALDGMHISLELGAN
                     DGAPAAGVIVPLKFRLADARTGAPVRDLEPYLGAWGHTLILNADQSHYLHSHPTEMLS
                     DGYDPARSRGGPEVEFKAMFPEAGDYRIWTQFQRAGRVTTVS"
     gene            complement(1206..2096)
                     /locus_tag="VF240_00020"
     CDS             complement(1206..2096)
                     /locus_tag="VF240_00020"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX7173652.1"
                     /translation="MEIKKVGVLGCGLMGSGIAQTAATAGFDVVVREVTSDLCERGFT
                     GIDKSLARFAEKGTITADQQREIRARLSGTTALEDLSDCDVIIEAIIENLDTKRETFK
                     RLDEVCKPETILASNTSSLSITEMMTATSAERQKRFVGLHFFNPVPLMKLVEVVRTVL
                     TDEAVYEQAVEFGKKMGKVPVRAGDRSGFIVNRLLVPYLLDSIRALEEGAGSIVDIDN
                     GMKLGCGHPMGPLTLLDFVGLDTTYYIAEIMFTEFRERRFAPPPLLKRMVNAGLYGRK
                     SGRGFYDYADPKNPVPMNLS"
     gene            complement(2223..3572)
                     /locus_tag="VF240_00025"
     CDS             complement(2223..3572)
                     /locus_tag="VF240_00025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7173653.1"
                     /translation="MAEGRIGDEGAYAGRASVCRACGALVGAGETSCMMCGASVAASP
                     SSSAGLGPRAGGARDATLRYADAETRRFMRAVIARPAPFTFIFLSACLFLFLLMKSAG
                     VDENPAVLVTYGAKVNRLIDRGEWWRFITPIFLHGGPVIGWIHLLVNMYGLFMLGPYV
                     EKLYGSAKFTVFWILTGIGGTVASYLAVRPGRGDGALARFLFNTSDVPSVGASGALFG
                     LVGVLFVFGIKFRHELPEGFKRAFGTGMLPMIAINVFIGFSVDIIDNAAHLGGLATGV
                     LVALFTDFKRPGLRGPVAFAWHAAQGLALALVVLSFAMVAYNFERPTSNITYIQAMNE
                     GQEAFGKAVGEEADAGAAEAAAEKLGAVPPLDPEADRLREELRVIVERAARFAAEGKA
                     SPPQTEDALAERVRQVEQLVADFKEWEARSDAWVEREGPRLDIWLVKPTPAPEGGQQ"
     gene            3885..4421
                     /locus_tag="VF240_00030"
     CDS             3885..4421
                     /locus_tag="VF240_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173654.1"
                     /translation="MRKHLYAIVFVLSAATLAAGFQQPQPWVKLSPPGGAFSVMMPTK
                     PEESVETKDSPLGKYTTHLFITRAEGVIYIAGWVDYAPGVKLDVRGEINANRDNFLKG
                     VEAKATSEKEIRIDGHPGIEFTGESAKALFKARVYLVRNRPYMVAAAWGVGQAEPPAV
                     GTFLTSFALAKAARPGAD"
     gene            4789..7044
                     /locus_tag="VF240_00035"
     CDS             4789..7044
                     /locus_tag="VF240_00035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX7173655.1"
                     /translation="MRNQRLLITLLVLLSLFAPLMGAPLSPAGARGVSVQEGRADREG
                     RADKMTGVLLARARGARPGSRERTRVILSVTDDESAERASVALQSLGASVRTRLDDVG
                     VLVADVPVERLAEVAARDDVAWVSDDQEVRSLAAAPDNTSHAEVTTGASKLLPVGNGN
                     LARGGGGNGVGIAVLDSGISPPDAAEFAGYYWDESGGVLSLGLLLSQPVLESYSRIVK
                     KVNFTGEGVSADAYGHGTHTAGVAAGSGQASEDAAASTAGAPTYGGIATGAHLIDVRV
                     LNSQGTGRVSDVIAGIDWVIKNRAAHNIRVMNLSLGAAPTQSYRTDPLCQAVSRAADA
                     GLVVVAAAGNWGKDSGGRSIYGGILSPANSPRVITVGASNTQQTNRRSDDTITSYSSR
                     GPTLTDGLLKPDLVAPGNLIGAAETSVSSPTASNYSSETGGGGLIGGLLFGDTSATRP
                     RATGTYQILSGTSFAAPAVSGTVALMLEANPSLTPAMVKAVLMRTAQRLPVYEGKVMR
                     GEMTTVERIITEGAGELNTSAAVSVAKAIRKDANRARVGENLITNKNLTYQTLGATSA
                     IAGETLPLNNGVVWAEGVAFTDRLRLTSGYQLADGRVLSSGFVIADGYMTADGRYVMP
                     DGYVVSSGFAIGSGFAIGSGFAIGSGFAIGSGFAIGSGFAIASGFAIGSGFAIGSGFA
                     IGSSTCPSGASPCASYSSSFNYQAAWASNLIDPASAGLNEEQVMIQGEDALPLGVRRL
                     SATSSLFPRRR"
     gene            7123..9186
                     /locus_tag="VF240_00040"
     CDS             7123..9186
                     /locus_tag="VF240_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7173656.1"
                     /translation="MAQERAERKGLAYEVTVIAAGLALWLGSVAGLWMSYSLRDNLIF
                     LALVPLIVVAGMFPNVFPMPSGLRLTKERITFTLSDGIILLVACWYGLAPAVFISGLD
                     GLISSRRAVRRLSSNVFSFGMLSLVAGASSVCLSAVLRHGFGESGTGPQQSFPAVAVS
                     LLAASVVHIVVNTGLFAAFFALRRGESVRGRWRESFLWAAPIFLPTSAIASLLYIALQ
                     FDALLTAVIGAPLLLALYFGQRQYRDSMQERIDLVERASRERMSVMEKAHRQTIEALA
                     VAINAKDEVTHGHVLRVQIYAAGVARLLGCDDSEVEALRAGALLHDIGKIAVPDYILN
                     KPGKLTADEFDKMKLHTVAGAQILSRVEFPYPVVPVVRHHHERFDGKGYPDGLKGEDI
                     PLTARILSVVDCFDAVREDRQYRKAMTRDEAIDFIMQGSGTQYDPRVVGTFITHLPEF
                     EAEIQAHRDDPAENYGIAPMEQLSAAALLVPPAAGLAEADAAVRTTGVELGRENSEAL
                     DDMALALKGAPTEGEIISTFVEALAALVPYDTCALTLAASDAGENRVVRAAGRNASLL
                     AGRAVAPGEGVTGWVLANRKAFFNADPRLDLPAESAADFADYRTLGVAPLTHGEKFFG
                     AVSLYSAALATYTAEQQRLLQAAASLAAAALADIGDDGARLPCPLGAPRNDPAATPLE
                     STLTH"
     gene            9376..9906
                     /locus_tag="VF240_00045"
     CDS             9376..9906
                     /locus_tag="VF240_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7173657.1"
                     /translation="MTEDTNAQASESPNKQPRAGAGALASPARRALIVARAGQHRLAV
                     FADESEGVSKFADPAPLPSAPPAVLGVVAVRGRMRTLIDPLPLLQPPTAPLTSPDEAA
                     PTLALLLKGDEQLALAVAGVERAADIPADSILPLEPAHDFARGTVTLDGAHVLVLDPA
                     RLFEAAMRGHERRRGR"
     gene            10033..>12063
                     /locus_tag="VF240_00050"
     CDS             10033..>12063
                     /locus_tag="VF240_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173658.1"
                     /translation="MNEHSFATLEYEGLRALVAERAQTPMGRERAVALAPLADATAVR
                     RELRAVSECIELRRRGAALSFREVADPSQSLARLPIEGATLDPVSLLELARLCAQAAD
                     ARDAIHAEREACPVLWEEVADLPRTLVALAARVQSKILPSGELDDRASPELARIRHDI
                     VRLRSQITRSLESLMRRSEEAIQDSLVTVRNDRYVIPVRSDHRGRVGGVAHGFSSSGA
                     TVFVEPLETIDSNNELQALRETEEREIARILSTLTEELRAALPEIAAAARAVAALDFT
                     NAKAALSAHFRSVEPEIDDAGALELAEARHPLLEENLRAAGGEVVPVSFTLDAERPVM
                     VISGANAGGKTVVLKTAGLLSLMALSGLHVPARAARVPFYGAVLADIGDRQSIAANLS
                     TFTAHVANIRRMTEICADTNGHALVLLDEAGTGTDPEEGSALGVAVVDYFRRRCGAHV
                     IATTHYSGLKMYAANEEGVQNASVEFDERTLEPTYRLLVGVAGSSSGLEIARRFRLPE
                     EIVGAASARVGEATHAAEEYLRRIKREAEEAETLRRALEEERAAVAERYARLDRDAER
                     RERERQAEFAREMEAAVREFESRSRELYSKIEDRAERARVERETERRAAELRREAAQK
                     RSATARTATAGGGTTEGGDAAMPTTAGGVRVVRQGREVGKSKAGAAGEGDELR"
BASE COUNT         1912 a         4278 c         4012 g         1861 t
ORIGIN      
        1 gagacggtcg tgacgcgtcc cgcgcgttgg aactgtgtcc agatgcggta gtcgccggcc
       61 tccgggaaca tcgccttgaa ctcgacctcc ggcccgccgc gcgaacgtgc ggggtcgtag
      121 ccgtcggaga gcatctcggt cgggtgtgag tggaggtagt gcgactggtc ggcgttgagg
      181 atgagcgtgt ggccccacgc cccgaggtac ggctcaaggt cgcgcacggg tgcgcccgtg
      241 cgcgcgtccg ccaagcggaa cttcagcggt acaatcactc ctgccgcggg cgcgccgtcg
      301 ttcgcgccaa gctcaaggct gatatgcatt ccgtcgagcg ccttcgtgag cgtcgtatcc
      361 ggcgtgagta tgacggcggc gggcgcgacg tgtcggggag tgctgcggtg gtttgccgtc
      421 gccagttcct gatgcacgag ctgcggcgtg ccgccggcgg ggaagaagtc cgagtgaagt
      481 ttgtacgacc cctcgcgcgg gagcgccgtc tcgaccgtga agctgccgtc cgcgtcgagc
      541 tgcgggtgga tgtgttgata ctcgcgcaag tcgcggctga tgacgaacag gtggaagagc
      601 cgctcgtggt tgagcacgaa gctcttgacc ggcgccgctg tttgcggatg ggtgacgacg
      661 aagcgcaggc gcacacgctc gttcgccttc ggccgggcgg gcgtcgcggt catgtcgagg
      721 cggtagttgc cgcgcaccga cgggttgagc gaccgaagcg gcatcccgca cttcgggcac
      781 acgccctcct gtgcctgtcg gacttcgggg tgcatcgggc aggtgtgctc gtaggtcggg
      841 gccagcagct ccaacgcccg cgcgcgctcg gcggccgaca gcgaggccca gttcgacggc
      901 ggccccgtct cactctgtgg agcggacttg gccgccgcct tggccaccag cttcatcttg
      961 cattgcggac aactgccggg cgcgtgtgag gtaactgccg ggtgcatcgg gcagacgtag
     1021 gcggccggag tctgtgacgg cgactgcgtc tgcgtcgagg ccgtgggcgg aagcgcgaag
     1081 ccgaagaggc aggcgctcag cagtagcgtg cggactgtgc gcgacatggt aagccctcgc
     1141 aagattttcc gaacgcgtcc cgaccgccgt caatcgccgg ccgggacgcg tcggcgggag
     1201 cgtgctcacg agagattcat cggcacgggg ttcttcgggt cggcgtagtc gtagaagccg
     1261 cggcccgact tgcggccgta gaggccggcg ttgaccatgc gcttgaggag cggcggcggg
     1321 gcgaagcggc gctcgcgaaa ctccgtgaac atgatctcgg cgatgtagta ggtcgtgtcg
     1381 aggccgacga agtcgagcag cgtcagcggc cccatcgggt ggccgcagcc gagcttcatg
     1441 ccgttgtcga tgtccacgat ggagccggcg ccctcttcga gcgcgcggat ggagtcgagc
     1501 aggtagggga cgagcaggcg gttgacgatg aagcccgaac ggtcgccggc gcgcacgggc
     1561 accttgccca tttttttacc gaactcgacc gcctgttcgt agaccgcctc gtccgttaag
     1621 accgttcgca ccacctcgac gagcttcatc agcggcacgg ggttgaagaa gtgcaagccc
     1681 acaaaacgct tctgccgctc cgcggaggtg gccgtcatca tctcggtgat cgagagcgaa
     1741 gaggtgttgg aggcgaggat ggtctcgggc ttgcacacct catcgagacg cttgaaggtc
     1801 tcgcgcttgg tgtcgagatt ttcgatgatg gcctcgatga tcacgtcgca gtccgagagg
     1861 tcttcgaggg cggtcgtgcc gcttaagcga gcgcgaatct cacgctgctg gtcggccgtg
     1921 atcgtcccct tctcggcgaa cctcgcgagc gacttgtcga tgccggtgaa gccgcgttca
     1981 cacagatcgc ttgtgacctc gcggacgacc acgtcgaacc cggcggtggc ggccgtctgc
     2041 gcgatgcccg atcccatcag gccgcagccg agcacgccga cttttttgat ctccataaat
     2101 tgcttctcct gaataccgtg aatcgtaaat cgtgaatcgt aaatcgattg accgtgaatc
     2161 gcgtgcggga cgaatggttt taccagcggc gagcggcgcg tttcaagtcg cgcacgcgcg
     2221 gctcactgct gtccgccttc gggcgcgggc gtcggcttga cgagccagat gtctaagcgc
     2281 gggccttccc gttcgaccca ggcgtcggag cgcgcctccc actctttgaa gtcggccacc
     2341 aactgctcca cctgccggac gcgttccgcg agcgcgtctt cagtctgcgg cggcgacgcc
     2401 ttcccctcgg cggcgaagcg ggcggcgcgc tcgacgatga cgcgcaactc ttcgcgcagg
     2461 cggtcggcct cggggtcgag cggcggcacg gcccccaatt tctccgcggc cgcctcggcc
     2521 gcccccgcgt ccgcctcctc gccgaccgct ttcccgaacg cctcctgccc ctcgttcatc
     2581 gcctgaatgt aggtgatgtt cgatgtcggg cgctcgaagt tgtaggcgac catcgcgaag
     2641 ctgagcacga cgagcgcgag cgccagcccc tgcgccgcgt gccacgcgaa ggcgacgggg
     2701 ccgcgcaacc ccggccgctt gaagtccgtg aagagcgcga cgagcacgcc cgtggcgagg
     2761 ccgccgaggt gcgccgcgtt gtcgatgatg tccacgctga agccgatgaa gacgttgatg
     2821 gcaatcatcg gcagcatccc cgtgccgaaa gcgcgcttga acccctcggg cagctcgtgc
     2881 cggaatttga tgccgaagac gaagagcact cccaccagcc cgaagagcgc gcccgatgcg
     2941 cccaccgagg gcacgtcgga ggtgttgaac aggaagcggg cgagcgcccc gtcgccccgt
     3001 ccgggccgca ccgccagata gctcgccacc gtcccgccga ttcccgtcag tatccagaag
     3061 accgtgaact tcgccgaccc gtaaagcttc tcgacgtaag gcccgagcat aaagaggccg
     3121 tacatgttca ccagcaggtg tatccagccg atgacggggc cgccgtgcag gaagatgggc
     3181 gtgatgaagc gccaccactc gccgcggtcg atgagccggt tgaccttcgc gccgtaggtg
     3241 acgaggacgg ccgggttctc gtccacgccc gccgacttca tcagcagaaa gaggaagagg
     3301 cacgccgaga ggaagatgaa ggtgaaaggc gcggggcgtg cgatgacggc gcgcatgaag
     3361 cggcgcgtct cggcgtcggc gtagcgcagc gtcgcgtcgc gggcgccgcc ggcgcgcggc
     3421 cccagcccgg cggacgacga gggagaggcc gcgacgcttg cgccgcacat catgcaactg
     3481 gtctcgcccg cgcccacgag tgcgccgcag gcgcgacaca cgctcgcccg ccccgcatac
     3541 gcgccctcgt ctccgattcg accctctgcc aaacccctcc tacttcgccg cccgcgcccc
     3601 acgcgtcgcg agtcgcgcgg cctcgcctct tcggacgagc cagcgcgagc gcggcccgcg
     3661 cggcgcggcg gccgccgccg tggatgagac cgccggtcgt cctcaatccg aattgtctgg
     3721 aagtttccct cgcgtcgatt ctacaacgcg cctcggggcg gtgtcatgcg gcacgcgcgc
     3781 ccgcgcgccc attgactggg gccgggcgca gattgtataa gccttctgct cgccgcgcgc
     3841 gcccctcgcc tgagtttccc gaatccacca gcgaaaggac tccgatgaga aaacatttgt
     3901 acgccatcgt gttcgtgctg tcggcggcga cgctggccgc cggcttccaa cagccgcagc
     3961 cgtgggtgaa gcttagcccg ccgggcggcg ccttctccgt gatgatgccg accaagcccg
     4021 aggagtctgt cgagacgaaa gacagcccgc tcgggaagta cacgacgcac ctgttcatca
     4081 cgagggcgga gggcgtcatc tacatcgccg ggtgggtcga ttacgcgccg ggcgtgaagc
     4141 tcgacgtgcg cggcgaaatc aacgccaacc gcgacaactt cctcaagggc gtcgaagcga
     4201 aggcgacctc ggagaaggag atcaggatcg acggtcaccc cggcatcgag ttcacgggtg
     4261 agagcgctaa agcgctcttc aaggcgcggg tctacctcgt ccgtaaccgc ccgtacatgg
     4321 tcgccgccgc ctggggcgtg gggcaggccg agccgccggc cgtcggcacg ttcctcacct
     4381 ccttcgcgct cgcgaaggcc gccagacccg gcgccgactg agcgcgctcc gcacgacccg
     4441 caacgcacgc gaaaataaag agaggcggcc caacgaacgg ggccgcctct cccgtcccat
     4501 cgccgccttc cgccgcggca ctcccttccc aatcgtcgcc gatgcgtcga gacgcgcccc
     4561 ggcctgcgcg tcagcgcgcg gctcgttcgg tttgaccgaa cgcgttgacc gaaatatccg
     4621 tcaggacgac gtgcgacagt tgccgcccct cccgaaccgc ccgctatttc ccactgtaga
     4681 ggctccacgc cggcggcacg cgccttgaat gactagcgtg ggtttccctc gtgcccggaa
     4741 tgaaaaagtg aagagccaga cttttgtctg gatgtgagta agtcatcaat gcgtaatcaa
     4801 agattattaa tcaccctgct cgtgttgctc tccctgttcg cccccttaat gggcgcgccg
     4861 ctctcgccag cgggggcgag aggcgtgtct gttcaggagg gtcgcgccga tagggaaggc
     4921 cgcgccgaca agatgacggg cgtgcttctg gcgcgcgcac gcggcgcgcg ccccggcagc
     4981 cgtgaaagga cgcgcgtcat cctcagcgtg acggacgacg agtcggccga gcgcgcgagc
     5041 gtggccctcc agagtctcgg cgcgagcgtt cgcacgcgtc tcgacgatgt gggcgtactg
     5101 gtcgccgacg tgcccgtcga gcgacttgcg gaagtcgccg cgcgcgacga cgtggcgtgg
     5161 gtctcggacg atcaggaggt gcgctcgctc gcggccgcgc ccgacaacac gagccacgcc
     5221 gaggtgacga ccggcgccag caaactgttg cccgtgggca atggcaacct cgcccgcggc
     5281 ggcggcggca acggcgtcgg catcgccgtc ctcgacagcg gcatcagccc gcccgacgcc
     5341 gccgagttcg ccggctacta ctgggacgaa agcggcgggg tgctgagcct cggcctgttg
     5401 ctgtcgcagc ccgtgctgga gtcctacagc cgcatcgtca agaaggtgaa cttcaccggc
     5461 gagggcgtga gcgccgacgc ctacggccac ggcacgcaca cggcgggcgt cgccgccggc
     5521 tccggtcagg cgagcgaaga cgcggccgcc agcaccgcgg gcgcgcccac atacggcggc
     5581 atcgccacgg gcgctcactt gatcgacgtg cgcgtgctca actcgcaggg gacgggccgc
     5641 gtctccgatg tcatcgccgg catcgactgg gtcatcaaga accgcgcggc ccacaacatc
     5701 cgcgtcatga acctctcgct cggagccgcg ccgacgcaga gctatcgcac cgacccgctg
     5761 tgtcaggccg tctcgcgtgc ggcggacgcc gggctggtcg tcgtcgcggc ggccggcaac
     5821 tggggcaaag actcaggcgg tcgctccatc tacggcggca tcctctcgcc ggccaactcg
     5881 ccgcgcgtca tcacggtcgg cgcgtccaac acgcagcaga ccaaccgccg ctccgacgac
     5941 accatcacga gctactcctc gcgcgggccg acgctcacgg acggcctgct caagcccgac
     6001 ctcgtcgcgc ccggcaatct catcggcgcg gccgagacat ccgtctcctc gcccaccgcc
     6061 agcaactact cctcggagac gggcggcggc ggcctcatcg gcggcctcct cttcggcgac
     6121 acgagcgcca cccggccgcg cgccacgggc acctaccaga tactaagcgg cacctctttc
     6181 gccgcgcccg ccgtctccgg caccgtcgcc ctgatgctcg aagccaaccc gtcgctcacg
     6241 ccggcgatgg tcaaggccgt cctgatgcgc acggcgcagc gcctgcccgt ctacgagggg
     6301 aaggtcatgc gcggcgagat gacgaccgtc gagcgcatca tcacggaggg cgccggcgag
     6361 ttgaacacga gcgcggccgt cagcgtcgcc aaagcgattc gcaaggacgc caaccgagcg
     6421 cgcgtcggtg agaacctcat caccaacaag aatctgacct accagacgct cggcgccacg
     6481 tcggccatcg cgggcgagac gctgccgctc aacaacggcg tcgtctgggc cgagggcgtg
     6541 gccttcaccg accgcctgcg cctgacgagc ggctaccagc tcgccgacgg ccgagtgctc
     6601 tcgagcggct tcgtcatcgc cgacggctac atgaccgccg acggccgtta cgtcatgccc
     6661 gacggctacg tcgtaagcag cggctttgcc atcggcagcg ggtttgctat cgggagtggt
     6721 ttcgccatcg gtagcggttt cgccatcggc agcggctttg cgattggcag cggcttcgcc
     6781 atcgcgagcg ggttcgcgat tgggagtggc ttcgcgattg ggagcggctt cgccatcggc
     6841 agcagcacct gccccagtgg ggcctccccc tgcgcctctt acagctcaag cttcaactat
     6901 caggcggcgt gggcctcgaa cctgatcgac cccgcctccg ccggccttaa cgaagagcag
     6961 gtgatgattc agggcgagga cgccctgccc ctcggcgtcc gccgcctgag cgccacaagc
     7021 tccctcttcc cgcgccgccg ctaacccctc acgtcgaaag ggggccgccc cgaagtatcc
     7081 ggggcgcgcg cccccgcgaa cattccccgg agggaacgag gtatggctca agagagggct
     7141 gagagaaagg gactcgctta cgaggtcacg gtgatcgcgg cgggactcgc cttgtggctc
     7201 gggtcggtcg ccggcctgtg gatgagctac agcctgcgcg acaacctgat ctttctggcg
     7261 ctcgtgccgc tgatcgtcgt cgccgggatg ttcccgaacg tcttccccat gccgtcgggc
     7321 ctgcggctca cgaaggagcg catcaccttc acgctctccg acggcatcat cctgctcgtc
     7381 gcctgctggt acgggctggc cccggccgtc ttcatctccg gactcgacgg actcatctcc
     7441 tcgcgccgcg ccgtccgccg cctctccagc aacgtcttct ccttcggcat gctctcgctt
     7501 gtggccgggg ccagctccgt gtgcctgtcg gcggtgctgc gccacggctt cggcgagtcg
     7561 ggcacggggc cgcaacagag tttcccggcg gtcgccgtct cgctgctggc ggcgagcgtc
     7621 gttcacatcg tcgtcaacac ggggctgttc gcggccttct tcgcgctgcg gcggggggag
     7681 tccgtcaggg ggcgctggcg ggagagcttt ctgtgggccg cgccgatttt tttgccgacg
     7741 agcgccatcg ccagcctgct ctacatcgcg ctccagttcg acgcgctgct cacggccgtc
     7801 atcggcgcac cgttgctcct cgcgctctac ttcgggcagc gccaataccg cgacagcatg
     7861 caggagcgca tcgacctcgt cgagcgcgcg agccgcgagc gcatgagtgt gatggagaag
     7921 gcgcaccggc agaccatcga ggcgctcgcc gtggccatca acgccaaaga cgaggtgacg
     7981 cacgggcacg tcctgcgcgt gcagatttac gcggccgggg tggcccgcct tctggggtgc
     8041 gacgactcag aggtcgaggc gctgcgggcc ggcgcgctcc tccatgacat cggcaagatc
     8101 gccgtccccg actacatcct caacaaaccg ggcaagctca ccgccgacga gttcgacaag
     8161 atgaaactac acaccgtggc cggcgcgcag attctcagcc gcgtcgagtt cccctacccc
     8221 gtagtgccgg tcgtgcgtca ccaccacgag cggttcgacg gtaagggtta tcccgacggc
     8281 ctgaaaggtg aggacatccc gctcaccgcg cgcatccttt ccgtcgtcga ttgcttcgac
     8341 gccgtgcgcg aagaccggca gtatcgcaag gcgatgacgc gcgacgaggc catcgacttc
     8401 atcatgcagg gcagcggcac gcagtacgac ccgcgcgtcg tgggcacctt catcacgcac
     8461 ctgcccgagt tcgaggccga gatacaggcg caccgcgacg accccgcgga gaactacggc
     8521 atcgcgccga tggaacaact ctcggcggcg gcgcttctgg tgccgccggc ggcgggcctg
     8581 gccgaagcgg acgcggccgt ccgcacaacc ggcgtcgaac tggggcggga aaacagtgag
     8641 gcgctcgacg acatggcgct cgcgctcaag ggcgcgccga ccgagggcga aatcatctcg
     8701 accttcgtcg aggcgctggc cgcgctcgtg ccctacgaca cgtgcgcgct gacgttggcg
     8761 gcgagcgacg cgggcgagaa ccgggtggtg cgcgcggcgg gccgcaacgc cagtctgctc
     8821 gcgggccgcg ccgtcgcgcc cggcgagggc gtcacgggat gggtgctcgc caaccgcaag
     8881 gcgtttttta acgccgaccc cagactcgat ctcccggccg agtcggccgc cgacttcgcc
     8941 gactaccgca cgctcggcgt cgcgcccctc acccacggcg aaaagttttt cggcgccgtc
     9001 tccctctact cggcggcgct cgccacctac acggccgaac agcagcgcct cctgcaagcg
     9061 gcggcgtccc tcgcggccgc ggcgctcgcc gacatcggcg acgacggcgc acgtcttccc
     9121 tgtcccctcg gcgccccacg aaatgacccg gccgcgaccc cgctcgaatc cactcttact
     9181 cactgatcgg cgcaccccgg tatgtttgcg gacaagcgcc accatggcag aggcggtgat
     9241 atcgtccatg gtcttttttc ctctccttgt gccgcgattt caccacgcgc cgcgcgacgg
     9301 gcgtgtgcgt tgacaccctc ccggcgccgt gctagaaccg cttcggtctc tcacgcccga
     9361 aagcttttcc agacgatgac agaggacacg aacgcacagg ccagcgagtc tccaaacaaa
     9421 caaccccgtg ccggcgcggg cgcgctcgct tcgccagccc gccgcgcact gatcgtcgcg
     9481 cgcgccggac aacaccggct cgcggtcttc gccgatgagt ccgagggcgt ctcgaagttc
     9541 gctgaccccg cgccgctgcc ctccgcgccg cccgccgtgc tcggcgtcgt cgccgtccgc
     9601 ggccgcatgc gaacgctcat cgacccgctc ccgctcctcc agcctcccac cgcgccgctc
     9661 acttccccgg acgaggcggc gcccaccctc gccctccttc tcaagggaga cgaacagctc
     9721 gcgctcgccg tcgcgggcgt cgaacgcgcc gccgacatcc cggccgactc aatcctcccc
     9781 ctcgaacccg cacacgactt cgcccgcggc acggtcaccc tcgacggagc ccacgtcctc
     9841 gtcctcgacc ccgcgcgtct cttcgaggcc gccatgcgcg gccacgagcg ccgcaggggg
     9901 cggtgaaagc aaaaagccgt caatcgtcaa tcgtcaatcg tcgttgcagc tttgacgagc
     9961 acccgcgatt aacggcgagt cacgcccgaa acaggtttca cgattgacga tttacgattt
    10021 actatttacg aaatgaacga acactccttc gccacgctcg aatacgaggg cctgcgcgcg
    10081 ctcgtcgcgg agcgcgcgca gacgccgatg gggcgcgagc gcgccgtggc cctcgcgccg
    10141 ctcgccgacg cgacggccgt gcgccgcgag cttcgcgccg tctccgagtg catcgagctg
    10201 cgccggcgcg gggccgcgct ctccttccgc gaggtggccg acccctcaca gtcgctcgcg
    10261 cgcctgccga tagagggcgc cacactcgac cccgtctcgc tcctcgaact cgcgcggctg
    10321 tgcgcgcagg ccgccgacgc gcgtgacgcc atccacgccg agcgcgaggc ctgccccgtg
    10381 ttgtgggaag aggtcgccga cttgccgcgc acgctagtcg ccttggccgc gcgcgtgcag
    10441 tcgaagattt taccgagcgg cgaactcgac gaccgcgcca gcccggagct tgcgcgcatc
    10501 cgccacgaca tcgtccgcct gcgctcgcag atcacgcgct cgctcgaatc cctgatgcgc
    10561 cgctccgagg aggccattca ggacagccta gtcaccgttc gcaacgaccg ctacgtcatc
    10621 cccgtccgct ccgatcaccg cgggcgcgtg ggcggcgtcg cgcacggctt ctcctcctcg
    10681 ggcgccaccg tcttcgtcga gccgctggag accatcgact ccaacaacga acttcaggcg
    10741 ttgcgcgaga ccgaggagcg cgagatcgcg cgcatcctct ccacactcac ggaggagttg
    10801 cgcgccgccc ttcccgaaat cgcggcggcc gcccgcgccg tcgccgcgct cgacttcaca
    10861 aacgcgaagg ccgcgctctc cgcgcacttc cgctccgtcg agccggagat cgacgacgcg
    10921 ggagcgctcg aactcgcgga agcgcgacac cctctgctcg aagagaattt gcgagcggct
    10981 ggtggcgagg tcgtccccgt ctctttcacg ctcgacgcgg agcggcccgt gatggtcatc
    11041 tccggcgcga acgcgggcgg gaagacggtc gtgctcaaga cggccgggct gctgtcgctg
    11101 atggcgctct cgggcctgca cgtgccggcg cgtgcggcgc gcgtcccctt ctacggcgcc
    11161 gtgctcgccg acatcggcga ccggcagtca atcgccgcca acctctcgac cttcaccgca
    11221 cacgtcgcca acatccggcg catgacggaa atctgcgccg acactaacgg gcacgcactc
    11281 gtcctgctcg acgaggccgg caccggcacc gaccccgagg aaggctccgc gctcggcgtc
    11341 gccgtcgtcg attacttccg ccgacgctgc ggcgcgcacg tcatcgccac cacccactac
    11401 agcggcctga agatgtacgc ggccaacgag gagggcgtgc agaacgcgag cgtcgagttc
    11461 gacgagcgga cgctggagcc gacctaccgt ctgctcgtcg gcgtcgccgg ttcatcttcc
    11521 ggcttggaga tcgcgcgccg gttccgtctg cccgaagaga ttgtcggcgc ggcctccgcg
    11581 cgcgtcggcg aggcgacgca cgccgccgaa gaatacctcc ggcgcatcaa gcgcgaggcg
    11641 gaggaggccg agacgctccg gcgtgcgctc gaagaggagc gcgccgccgt ggccgaacgc
    11701 tacgcgcgtc tcgaccgcga cgccgagcgg cgcgagcgcg agcgtcaggc cgagttcgcg
    11761 cgtgagatgg aggcggcggt gcgcgagttc gagagccgct cgcgcgaact ctactcgaag
    11821 atcgaagacc gcgcggagcg cgcgcgcgtc gagcgcgaga ccgagcgccg cgccgccgag
    11881 ttgcggcgtg aggcggctca gaagcgttcg gccacggcgc gcacggcgac ggccggcgga
    11941 gggaccactg agggcggcga tgcggcgatg ccgacgactg cgggcggcgt ccgcgtcgtc
    12001 cggcaggggc gtgaggtcgg gaagtcaaag gcgggcgcgg ccggcgaggg cgatgagttg
    12061 cgc
//
LOCUS       DASVES010000003         9281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_188556, whole genome shotgun sequence.
ACCESSION   DASVES010000003 DASVES010000000
VERSION     DASVES010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188556"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VF240_00055"
     CDS             complement(<1..353)
                     /locus_tag="VF240_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173659.1"
                     /translation="MIRIIFGLLLFLPAAHAAFGQEVKRDARLIHPGAACASVPVRAP
                     EPSSAARREMEAKLAEARARYEKTPNDADAIIWLGRRTAYTGRFEEAVRIFSEGIRKH
                     PRDARLYRHRGHRYIT"
     gene            complement(364..1611)
                     /locus_tag="VF240_00060"
     CDS             complement(364..1611)
                     /locus_tag="VF240_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7173660.1"
                     /translation="MRRAINAVLFLLLAFAVVASAQTADGEEVGRLINDGASAYRAGR
                     YVEAQKLFEKALELDPEGRRHAHSKHLTLFIARALHQQYRPHDNAPENRAKAEAAVAA
                     YKRVLADERFHEKHHEDAFTAVAYLYRLLGEKEKEDRWILTRATDPSAPNGKRSDAYT
                     VLASKQWDCGYQITERKENKQTIDQGDRGIVVRYKKPGAQRDFDVAQGCVREGLGLIE
                     QALTLNPDSVNAWSYKTNLLRERAKLAQMEGDAAAQAEYGRLADEAQARHTKLQEGAA
                     EKREEVPKAGARVGPVGPRAESGATSPYPAPTPPAATQEGGTPRRRVTVSAGVLNGKA
                     VSKPAPSYPKEAKEAGVSGLVTVQVTIDEEGAVAVAEAVGGHTLLHAAAVEAVRRWRF
                     SPTRLSGQPVKVTGVVILNFVLP"
     gene            complement(1624..2337)
                     /locus_tag="VF240_00065"
     CDS             complement(1624..2337)
                     /locus_tag="VF240_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7173661.1"
                     /translation="MARSEYLSRFPVVEVQQTFYHPPRVETLRRWRAEAPRGFEFTLK
                     AWMLITHEARSPTYRRLKRELTEEEREGCGGFRDSEIVREAWEVTRACAEALKARRVL
                     FQCPASFRPTPENVARLRAFFRSTERGALRFHWEPRGEWPDALLRELCSELDLVHVVD
                     PLKTRTVTPRECYFRLHGRRGGRYTHEQDELEELLSMLPRGGRVSSYVFFNNVRMLED
                     ATRFQELARGAGADDFSEV"
     gene            2749..2919
                     /locus_tag="VF240_00070"
     CDS             2749..2919
                     /locus_tag="VF240_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173662.1"
                     /translation="MREMVSSFAQLAAIVMVLITLALSTAAVAQWAEADASAQGEGAA
                     PLALQVAAVSSR"
     gene            2986..5619
                     /locus_tag="VF240_00075"
     CDS             2986..5619
                     /locus_tag="VF240_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7173663.1"
                     /translation="MAIEITSIPEQRRLPRLRRARSLKIRAQAERADTRRRLLRFAAI
                     ALAAAACVSLVTVFYLYRSHARVIDERLASGYLTSRAGLYAAPRTLRKGQGITSARLV
                     EMLKRAGYVEGDAGAGMPSAVWSGSFRAGDGHVEILPRRAHEAGGRASFGAVRVEFNN
                     RGRIAAVRGDGAEIDSYTLEPEPLAQDVSMKTAERPALSYEDLPPTLVQAILAIEDRR
                     FFEHNGVDAQGLARALLSWGDVKEDQKQGGSTITQQLVKNTYLTPERTLRRKMNEAIL
                     ATALERRLSKQDILALYCNEIYLGQRGSMAVRGVDQAARVYFGKELKELTLAEAATIA
                     GMIQSPGRYAPDRHAERARLRRNTVIDAMLRDGVITPEQAASASDEAVSVVPAEEANA
                     AAPYFIDYVNRAVEARSGGDEEEENHLRVYTTIDLELQRLAEESVARQLERLEKVFKG
                     KRRPQAALVALDPQTGNVLAMVGGGSYAESQLNRVTDARRQPGSVFKPIVYAAALESG
                     ISPVTLSADAPREFSYDYRSKYKPANYGGGYSMRDVTLRTGLVRSLNVVTVDVAMRTG
                     LSRVASYAERLGLPRPDAYPSLALGTTEATPLEVASAYAVFANGGSAVRASAVSRYED
                     VIDPAAATSRVTSREQVIKPATSYMITDMLTAVVDHGTARAARGVLNGVAIAGKTGTS
                     RDGWFAGYTPNLVCVVWVGFDDNEQLGLTGADSALPAWVDFMRGATELRPELGGEHFA
                     RPASITTVEIDAETGLLASDSCPHRERIAVSPALAPQSACLTHMEGYGELAVAGAADY
                     YDSSMIEAEVNNLEDAAADDSARPRLPEPMSVSDDSMPAPRPKPVTAVRVERGRNGRP
                     VLTNEVRVYETEGAGSKGWNH"
     gene            complement(5902..6285)
                     /locus_tag="VF240_00080"
     CDS             complement(5902..6285)
                     /locus_tag="VF240_00080"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7173664.1"
                     /translation="MIVSIGIDIIEVGRVRQALARTPRLRARLFTEGERAYCEGRGAA
                     SAQHFAARFAAKEAALKALGTGWRGRITWHDVEVIRQETGEPALLFHGEAREIFRRLG
                     ATLAHLSISHTSEHAVAQVILEKFS"
     gene            6272..7126
                     /gene="pgeF"
                     /locus_tag="VF240_00085"
     CDS             6272..7126
                     /gene="pgeF"
                     /locus_tag="VF240_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX7173665.1"
                     /translation="METITDETELSAAGFSWRESGAVRALTCEPLRRDGFTNAFSTRT
                     GGVSPMPEAALNLAGFNDDAAENIHENRRRFLSLLGGGGDWSLAACWQVHGSDVRVVR
                     EESEARSLAGAEEARCDALVTGVPRVLLGVKTADCVPVLLGDARTGACAAVHAGWRGT
                     LAEIVGRALERLSAEYGTLPADVRAAIGPAARGCCYEVGREVIDAFHEKFPRAGEWFK
                     ETREGHALVDLQRANLEQLVAAGVPEGRVHTLPLCTMCRTDLFFSYRREKKLHGRTGR
                     LLSVIGRG"
     gene            complement(7292..7894)
                     /locus_tag="VF240_00090"
     CDS             complement(7292..7894)
                     /locus_tag="VF240_00090"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX7173666.1"
                     /translation="MRRQLFAIQASIILLLGATIQAAPPVTTTPAPPTRAYTAAATPA
                     QPGIDVSGLFSVNKAQPGSVLQGAVILRIPDGYHINSNRPTNKFMIPTSVRVFPPRGV
                     RVGPVRYPRANVRSFGFAPDERLPVFEGAPAMRFDVTVPAGFKQDKLRMRVVVAYQAC
                     SDETCFRPAERDITLSLDVAGADEEVQRVNGHIFGRGRRR"
     gene            complement(8036..9127)
                     /gene="rlmN"
                     /locus_tag="VF240_00095"
     CDS             complement(8036..9127)
                     /gene="rlmN"
                     /locus_tag="VF240_00095"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7173667.1"
                     /translation="MEQERIHLTGLTRSELESFVEELGEPKYRGRQIFAALQRRRLRA
                     FDEMTDLPREFRTKLAERATASTLTVESRYLAQDGTRRYLLKTHDGLPVEAVFIPEER
                     RDTICFSSQSGCPLQCSFCLTAKLGLLRTLTAGEMVEQIVVALNDAYGVGTRTPRGTN
                     LVAMGAGEPFLAYEPLMQALGVMADPTGLHIVPNRVTVSTAGIVPRILELAERSARPH
                     LAISLAAPNDELRDELMPINKKWPIAELLAACRVFERSLRPGERFTFEYVMLDGVNDS
                     DEHARQLANLLNRHGLQRVKVNLIPHNPADPLPYRPSSEERVERFRAILASKGVDTFV
                     RRPRGRDIYAACGQLAARRDAAPLLVQLS"
BASE COUNT         1507 a         3111 c         3117 g         1546 t
ORIGIN      
        1 gtgatgtagc ggtggccgcg gtggcggtag agacgcgcgt cgcgcgggtg cttgcggatg
       61 ccctcggaga agattcggac ggcctcctcg aagcggcccg tgtaggccgt gcggcggccg
      121 agccagatga tcgcgtccgc atcgttcggg gtcttctcgt agcgcgcacg cgcttccgcc
      181 aacttcgctt ccatctcgcg ccgcgccgcg gacgacggct ccggcgccct gaccggcaca
      241 gacgcgcacg ccgcgccggg atgaatcagc ctcgcgtcgc gcttcacctc ctgcccgaag
      301 gccgcgtgcg ccgccggcag gaacaacagc aagccgaaaa ttattcttat catacgcgtc
      361 gcctcagggg agtacgaagt tgaggatgac tactcccgtg accttcacgg gttggccgga
      421 gaggcgcgtc ggcgagaagc gccaacgccg cacggcctcg acggccgccg cgtgcagcag
      481 cgtgtggccg cccacggcct ccgcgaccgc gaccgcgccc tcctcgtcga tggtgacctg
      541 caccgtcacc aggccggaga cgccagcctc cttcgcctcc ttcgggtagg agggcgcagg
      601 cttcgagacc gccttaccgt tgagtacgcc ggcggagacc gtcacccgtc gtctgggggt
      661 gccgccctcc tgcgtcgccg ccggcggcgt gggcgcgggg tacggggacg tggcgcctga
      721 ctccgccctc ggcccgacag ggccgacccg cgctcccgcc tttggcactt cctcacgttt
      781 ttccgccgcc ccttcttgca gcttcgtgtg cctcgcctgg gcctcgtcgg ccagcctgcc
      841 gtactcggcc tgcgcggcgg cgtcgccttc catctgcgcc agcttggcgc gctcgcgcag
      901 caggttggtc ttgtacgacc aggcgttgac gctgtcgggg ttcaatgtga gcgcctgttc
      961 gatgaggccc agcccctccc gcacgcaacc ctgagccacg tcgaagtcgc gttgcgctcc
     1021 gggcttcttg tatcggacga caatcccgcg gtcgccctgg tcaatggtct gcttgttttc
     1081 cttccgctcg gtaatctggt agccgcagtc ccattgctta ctcgccagaa cggtgtaggc
     1141 gtcggagcgc ttcccattcg gcgcggatgg gtcagtcgcg cgcgtcagta tccacctgtc
     1201 ctctttctcc ttctccccca acaaacggta gaggtaggcc acggcggtga aggcgtcttc
     1261 atggtgcttc tcgtggaacc tctcatcggc caggactctt ttgtacgccg cgacggccgc
     1321 ctcggctttg gcccggttct ccggcgcgtt gtcgtgcggc ctgtactgct ggtgaagggc
     1381 gcgggcgatg aagagggtga gatgtttact gtgggcgtgg cgcctaccct cagggtcgag
     1441 ttcgagtgcc ttctcgaaaa gcttctgagc ttcgacgtag cggccggccc tgtaggcgct
     1501 cgcgccatcg tttattagac gtcccacctc ctcgccgtcc gcagtctgcg cgctggcgac
     1561 gacggcgaac gctaagagga ggaacagcac ggcgttgatc gctcttctca tcgcttcgac
     1621 tccttacact tccgaaaagt catccgcgcc ggcgccgcgc gccaactcct gaaatcgcgt
     1681 cgcgtcttcg agcattcgca cgttgttgaa gaagacgtag gaggagacgc ggccgccgcg
     1741 cggcagcatc gagagcagtt cttcaagctc gtcctgttcg tgcgtatatc gaccgccgcg
     1801 ccggccgtgc agacggaagt agcactcgcg cggcgtgacc gtgcgcgtct tgagcggatc
     1861 gacgacgtgg acgaggtcta actccgagca cagttcgcgc aacaacgcgt cgggccattc
     1921 gccgcgcggc tcccagtgga aacgcagggc gccgcgctcc gtcgagcgga agaaggcgcg
     1981 caggcgcgcg acgttctcgg gcgtcggccg aaagctcgcg gggcactgga agagcacgcg
     2041 gcgcgccttc agggcctcgg cgcacgcgcg cgtcacttcc cacgcctcgc ggacgatctc
     2101 cgagtcgcga aacccgccgc acccttcgcg ctcctcttcg gtcagctccc gcttcaggcg
     2161 tcggtaggtg gggctgcgcg cctcgtgtgt aatgagcatc cacgccttga gcgtgaactc
     2221 gaagccgcgc ggcgcctccg cgcgccagcg ccgcaaggtc tccacgcgcg gcgggtggta
     2281 gaaggtctgc tgcacctcga cgacggggaa gcgactcaag tactcggagc gcgccatctg
     2341 aaagccgcag catccggttt taactttccg ggtagccatt aacggcggta agcgtaaaac
     2401 aatttcgacg aatcacaaag cccttcgacc gtccgccgct ttcgccgttc gtcgtggttg
     2461 ccactctccg ccggtgctct cgaaatccgc tttgggattg ctggaaaatt gaatcgtttt
     2521 gaaatggccc gccgccgctg cctcacgtaa aaatctgtat cgaaagcggc cgaggacacg
     2581 tgcgtcgcga cccggcgact tcgacgcgat aaatttttaa ttgcttctga agttgtgggt
     2641 tatgtgcgtc ggcgcggctt cgtcgaaaac tatcgcgggg ccggcatggc cgttgcaatt
     2701 ggcgtcttgg caaaccggaa ataattcgct aaggggagca ctgccaacat gagagaaatg
     2761 gtcagcagct tcgcgcaact cgccgccatc gtcatggttc tgattacgct ggcgctctcg
     2821 accgcggcgg tcgcgcagtg ggccgaggcc gacgcgtcgg ctcagggaga aggggccgcg
     2881 ccgctggcgt tgcaggtggc cgccgtctct tcccggtgac aagaatctgg tgacgattaa
     2941 gactttattc tcgacacacg ctgaaggcga cggcaaccac aacgcatggc aatcgagatc
     3001 acatcaattc ccgagcagcg gcgactgcca cgcctccggc gcgcccgctc cctgaaaatc
     3061 cgcgcgcagg ccgaacgcgc cgacacgcgc cgccgcctgt tgcgctttgc cgccattgct
     3121 ctcgccgccg cggcttgcgt ctcactcgtc acagttttct acctctatcg ctcacacgcc
     3181 agagtcatcg acgagcgcct cgcctcgggc tacctgacga gccgcgccgg cctctacgcc
     3241 gcgccgcgca cgctccgtaa gggacagggc atcacgagcg cgcgcctcgt cgagatgctg
     3301 aagcgcgccg gctacgtcga gggcgacgcg ggcgcaggga tgccgagcgc cgtctggagc
     3361 gggagcttcc gcgcgggcga cggacacgtc gagatactgc cgcgccgcgc gcacgaagcg
     3421 ggcggacgcg cgtcgttcgg cgcggtgcgc gtcgagttca acaaccgcgg gcgcatcgcg
     3481 gcggtgcgcg gcgacggcgc ggagatcgat tcctacacgc tggagccgga gcctctggcg
     3541 caggacgtgt cgatgaagac ggccgagcgg cccgcgctct cttacgaaga tttgccgccc
     3601 acgctcgttc aagccatcct cgccatcgaa gaccgtcgct tcttcgagca caacggtgtg
     3661 gacgcgcaag ggttggcgcg cgcgctcctc agttggggcg acgtaaaaga agatcagaag
     3721 cagggcggct caaccatcac gcagcagctc gtcaagaaca cctatctcac gcccgagcgc
     3781 acgctccgac gcaagatgaa cgaggcgatt ctcgcgaccg cgctcgaacg ccgtctgtcc
     3841 aagcaggaca tcctcgcgct ctactgcaac gagatttatc tcgggcagcg cgggagcatg
     3901 gccgtgcgcg gcgtggatca ggcggcgcgc gtctacttcg gtaaagagtt gaaggaacta
     3961 acgcttgccg aggcggcgac gattgccggc atgatccaaa gccccggccg ctacgcgccc
     4021 gaccgccacg cggagcgcgc gcgcctgcgg cgcaacaccg tcatcgacgc gatgctgcgc
     4081 gatggggtca tcacgccgga gcaggccgcg tccgcttcgg acgaagcagt cagcgtcgtc
     4141 cccgcggaag aagcgaatgc cgccgcgccc tacttcatcg actacgtcaa ccgcgccgtc
     4201 gaggcgcgct ccggcggcga cgaagaggaa gagaatcacc tgcgtgtcta cacgacgatt
     4261 gatctggagt tgcagcgtct ggccgaagag tcggtcgccc gccagctcga aagattagag
     4321 aaggtcttca agggcaagcg ccggccgcag gcggcgctcg tcgcgctcga cccgcagacg
     4381 ggtaatgtcc tcgcgatggt cggcggcggc tcctacgccg agtcgcagct caaccgcgtg
     4441 acggacgcgc gccgacagcc cggctcggtc ttcaaaccca tcgtctacgc cgccgcacta
     4501 gagagcggca tctcgcccgt cacactctcc gcggacgcgc cacgcgagtt ctcctacgac
     4561 tatcgctcga agtacaagcc ggccaactac ggcggcggct actcgatgcg cgacgtgacg
     4621 ctacgcacgg gcctcgtgcg ctcgctcaac gtcgtcaccg tggacgtggc catgcgaacg
     4681 ggcctctcgc gcgtcgcctc ctacgccgaa cgactcgggc tgccgcgtcc cgacgcctat
     4741 ccgtcgctcg cgctcggcac gaccgaggcg acgccgctcg aagtggcctc ggcctacgcc
     4801 gtcttcgcca acggcggctc ggccgtccgc gcgtcagccg tctcgcgcta cgaagacgtg
     4861 atcgacccgg cggcggcgac gagccgcgtc acgtcgcgcg agcaggtcat caagcccgcg
     4921 acgagctaca tgattaccga catgctcacg gccgtcgtcg atcacggcac ggcgcgcgcg
     4981 gcgcgcggcg tcctcaatgg cgtggccatc gccggcaaga ccgggacgag tcgcgacggc
     5041 tggttcgccg gctacacgcc gaacctcgtc tgcgtcgtct gggtgggctt cgacgacaac
     5101 gagcagttgg gactcacggg cgcggactcg gccttgcccg cgtgggtcga tttcatgcgc
     5161 ggggccacgg agctgcggcc cgaactcggc ggcgagcact tcgcccgccc ggcctcgatc
     5221 acgaccgtgg agatcgacgc cgagaccggg ctgctcgcct ccgactcgtg cccgcaccgc
     5281 gagcgcatcg ccgtctcgcc cgcgctcgcc ccgcagtccg cctgcctcac gcacatggag
     5341 gggtacggcg aactcgccgt tgcgggcgct gccgattact acgactcgtc gatgatcgag
     5401 gccgaagtga acaacctcga agacgccgcc gctgacgatt ccgcccggcc gcgtctgccc
     5461 gaaccgatgt ccgtctccga cgattctatg ccggctccga ggccgaagcc ggtgaccgcc
     5521 gtgcgcgtcg agcgcggcag gaacggacgg cccgtgctca cgaacgaggt tcgcgtctac
     5581 gagacggagg gcgcgggcag caagggctgg aatcattgaa acgcgcgggc gggacacgcc
     5641 tcgtgcgacc ctgagacgaa gccagccgcg tagcggcgac agaatgtagc ccacagcgcg
     5701 agccgtgggg acaagacgcc gtaaagattt ggaagccccg gaggggcgaa agaacatcga
     5761 gcttgattat gtgcaagctc gatgttcttt cgcccctccg gggctttcgt tttttaactc
     5821 gacgctgacc cacggctcgc gccgtgggct acattcttcc gcccctgacg gggctgacga
     5881 cacacgcggc cgaaatttcg tctaactaaa tttctctaaa atcacctgtg cgacggcgtg
     5941 ctcggacgtg tgagagatgg agaggtgtgc gagcgtggcg ccgaggcggc ggaagatttc
     6001 gcgcgcctcg ccgtggaaga ggagcgccgg ctcgcccgtc tcctgccgga tgacctccac
     6061 gtcgtgccac gtgatacggc cgcgccatcc cgtgccgagc gccttgagcg ccgcctcctt
     6121 cgcggcgaaa cgcgcggcga agtgctgcgc cgacgccgct ccgcgcccct cgcagtaggc
     6181 gcgctcgccc tccgtgaaga ggcgagcgcg caggcgcggc gtgcgcgcca acgcttggcg
     6241 cacgcgcccg acttcgataa tgtctatgcc gatggagacg atcacggacg aaacggaact
     6301 gtcggcggcc ggcttctcat ggcgcgagtc cggcgcggtg cgcgccctca cgtgcgagcc
     6361 gctccggcgc gacggcttca ccaacgcctt ctcgacccgc accggcggcg tcagccccat
     6421 gcccgaggcc gcgctcaacc tcgccggctt caacgacgac gcggcggaga acatccacga
     6481 gaaccgccgc cgcttcctct cgctgctcgg cggcggcggc gactggtcgc tcgccgcctg
     6541 ctggcaggtg catggctcag acgtgcgcgt cgtgcgggaa gagtcagagg cgcgctcgct
     6601 cgccggcgcc gaggaagcac gctgcgacgc gctcgtcacg ggcgtcccgc gcgtgctgct
     6661 cggcgtcaag acggccgact gcgtccccgt cctcctcggc gacgcgcgca ccggcgcgtg
     6721 cgcggccgtc cacgcgggct ggcgagggac gcttgccgag atcgtcggtc gcgctctcga
     6781 acgactgtcg gccgagtacg gcacgctccc cgcggacgtg cgcgccgcca tcggcccggc
     6841 cgcgcgcggc tgctgctatg aggtgggccg cgaagtcatc gacgccttcc acgaaaaatt
     6901 cccgcgcgcc ggcgagtggt tcaaagagac gcgcgagggc cacgcgctcg tcgatcttca
     6961 aagagccaac ctcgaacaac tcgtcgcagc gggagtcccc gaagggcgcg tccacacgct
     7021 cccgctctgc accatgtgcc gcaccgacct cttcttctcc taccggcgcg agaaaaaact
     7081 ccacgggcgg acggggcgac tgctctccgt catcggacga gggtgattga ctgcgcgttg
     7141 ggcggtcagt accgcgagcg gtagcgagcg ggtctggatg cgcgatacgg gtcgctcatc
     7201 ataggcatac ccgctcgcta ccgctcgcgg tactgtgcct gccgactcca ctcgttgagt
     7261 cgatgagtca atgaacagat gagtcgatca ctcacctgcg tcggccgcgg ccgaagatgt
     7321 ggccgttgac gcgctgaact tcttcgtcgg cgccagccac gtcgagcgag agcgtgatgt
     7381 cgcgctcggc gggccggaag caagtctcgt ccgagcaggc ttgataggcg acgacgacgc
     7441 gcatccgtag tttgtcctgc ttgaaaccgg cgggcacggt cacgtcgaag cgcatggcgg
     7501 gcgcgccctc gaagacgggc aggcgctcgt ccggcgcaaa cccgaagctg cggacgttgg
     7561 cgcgcggata cctgacgggg ccgaccctca ccccgcgcgg ggggaagacc ctgacggatg
     7621 tcgggatcat gaacttgttc gtcggccggt tcgagttgat gtgatagccg tccgggatgc
     7681 gcagaatgac ggccccctgc aagacgctgc cgggctgcgc cttgttcacc gagaagagac
     7741 cgctcacgtc gatgccgggt tgcgcgggcg tggccgccgc ggtgtaggcg cgcgtcggcg
     7801 gcgcgggagt agtggtgact ggcggcgcgg cctgaatcgt cgcgcccagc aaaaggatga
     7861 tgcttgcctg aatggcgaat aattgtcttc tcatgtttct ctcatctgtc ggaagttttc
     7921 gtcaattcta accgcgcgcg ccggaaaaca aaaaccgaag cgcgtcgcgc ggcggttgta
     7981 gtgatgcgcg tggggcgttg gagagttcgc cgccgaaggt gcgcggccga acaagtcagg
     8041 agagttggac gaggagtggg gcggcgtcgc ggcgcgcggc gagctggccg caggcggcgt
     8101 agatgtcgcg gccgcggggc cgtcggacga atgtatcgac gcccttcgat gcgagaatcg
     8161 cgcggaatcg ctcgacgcgc tcttccgaag agggccggta gggcagtggg tcggccgggt
     8221 tgtgggggat gaggttgacc ttcacgcgct ggaggccgtg acggttgagg aggttggcga
     8281 gctggcgcgc gtgctcgtct gagtcgttca cgccgtcgag catcacgtac tcgaaagtga
     8341 atcgttcgcc cggccgcaga gacctctcaa acacccggca cgcggcgagc agttcggcaa
     8401 tcggccactt cttgttgatg ggcatcagct cgtcgcgcag ctcgtcgttg ggcgcggcga
     8461 gcgagatggc gaggtgcggg cgcgccgagc gttcggcgag ttcgagtatg cgcgggacga
     8521 tgccggccgt ggagaccgtg acgcggttcg ggacgatgtg cagccccgtg gggtcggcca
     8581 tgacgccgag cgcctgcatc agaggttcgt aggcgaggaa cggctcgccg gctcccatcg
     8641 cgacgaggtt cgtgccgcgc ggcgtgcgcg tgcccacgcc gtaggcgtcg tttaaggcga
     8701 cgacgatctg ctcgaccatc tcgccggccg tgagcgtgcg cagcaggccg agcttggccg
     8761 tcaggcagaa gctacactgg agcgggcagc ccgactgcga ggagaagcag atggtgtcgc
     8821 gtcgctcctc ggggatgaag acggcctcga cgggcaggcc gtcgtgcgtc ttcaaaaggt
     8881 agcggcgcgt gccgtcctgc gcgaggtagc gcgactcgac cgtcagggtc gaagcggtcg
     8941 cgcgctccgc gagcttcgtg cgaaactctc tcggcaggtc ggtcatctcg tcgaaggcgc
     9001 gcaggcgacg gcgctggagc gccgcgaaga tttgccggcc gcgatacttc ggctcgccca
     9061 actcctcgac gaaagactca agctccgagc gcgtcagccc cgtcagatgt atccgctctt
     9121 gttccacgag ttatagttta caacgcgccg ggcacgcggg cacacccggg cgcgcgcacg
     9181 gccgtgtgat tgattgtgtg tgagggacgg cgcgcgggtg ttatattgcg cctccagcgt
     9241 gagtatcatc tgctgtaaca gtaccgcgag cggaagcctc g
//
LOCUS       DASVES010000004        13471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1742522, whole genome shotgun sequence.
ACCESSION   DASVES010000004 DASVES010000000
VERSION     DASVES010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13471
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742522"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..628
                     /locus_tag="VF240_00100"
     CDS             398..628
                     /locus_tag="VF240_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173668.1"
                     /translation="MNTKTPIKIIKSGERKRQAEASAQVAATAEKKTPQEAARDMVAT
                     VTDWVNEFQQKRRVETRQALRNFLTETTPQTS"
     gene            851..1027
                     /locus_tag="VF240_00105"
     CDS             851..1027
                     /locus_tag="VF240_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173669.1"
                     /translation="MTKTRKTQPKGREPRPVFDAIRKPAAPPGHPLTRAKPEERAHPA
                     ERKAKHKRSPDEPE"
     gene            1095..1634
                     /locus_tag="VF240_00110"
     CDS             1095..1634
                     /locus_tag="VF240_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7173670.1"
                     /translation="MDEITREFLTVARKYLREDYLPKIERCLERLTDEQVWWRAHETS
                     NSIGNLLLHLEGNARQWILCGVGGARDARVRQREFDERAQLPRAVLVEKLRATLDEVD
                     GVLASLTAREFDGGRRSIQGNDVSVLEAVFHVVEHFSMHTGQIILLTKTLTASDLAFY
                     DTSGGDPRPDWRRATPDDD"
     gene            1808..2092
                     /locus_tag="VF240_00115"
     CDS             1808..2092
                     /locus_tag="VF240_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX7173671.1"
                     /translation="MTYTVTDPCVNCRYTDCALVCPVEAFHLVDEPDRKLLVINPETC
                     IDCDACVPECPVEAIFPEDKVPEDQQHWTPLNAELCIGNPIQTEKMEAVC"
     gene            2215..2559
                     /locus_tag="VF240_00120"
     CDS             2215..2559
                     /locus_tag="VF240_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173672.1"
                     /translation="MSKLRTKKRRRRGGRLLGALGLVAGAVGTTFLLRRRGERKAQGG
                     LTTDDHAPRSTAADRWARPGMEATFRAELMPGRSRTERTFRVARLLPSGRVTLDGVAG
                     EHAESEFEQIRK"
     gene            complement(2649..3419)
                     /locus_tag="VF240_00125"
     CDS             complement(2649..3419)
                     /locus_tag="VF240_00125"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEX7173673.1"
                     /translation="MKIHLLPSSFDRAGGANPEQRLSCFLVDDCVAIDGGSIALNGDR
                     CRRVRDVIVTHPHMDHIATLPIFVDDNFALLETPVRVHATEEVVRLLRRDVFNGTIYP
                     PFHDFDNGRTRVLEYVPFRAGEEFAVAHLRVTAVAVNHIVPTVGLILKDTRATVAFSS
                     DTAATEEFWQVVNRLPRLDALLVEASFPNSLARLAEVSGHLTPDSLGRELAKLTQQGA
                     QILAMHIKPAYRERIVRELDALGVPGLSVMETGRDYSW"
     gene            complement(3479..4654)
                     /locus_tag="VF240_00130"
     CDS             complement(3479..4654)
                     /locus_tag="VF240_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX7173674.1"
                     /translation="MSDPARLGYNPALAPGPRAATRPRSGSAFRWVVAATAVLLAALI
                     GLVTLLLIGFSTGPVPFLTGLVVAALPAPVYVLLVLWIDRFESEPGWLLATAFFWGAL
                     VAVGVALIVNTLGMVAVDAAFGEEAADLYGLALSAPLVEEGTKALALFILYFWKRDEF
                     DGVLDGIVYASMVGLGFAMTENIKYYAEAALEGRALGTFVVRGMFSPYAHPLFTSMTG
                     IGLGLASHAARRSARVTLPFVGLALAVVLHSVWNTSLFFSDKLERGLIALVMYFLVMV
                     PVFTGVLMVAFFGLRKEGRILREHLAGDVERGTLARVEYERLCSVRRRLGASLGALFG
                     GGFSAWRDRLRLHGVASELAFHRHRVARGWTPRDSAAVERETHYLQSLVELRGRLSG"
     gene            5041..6126
                     /locus_tag="VF240_00135"
     CDS             5041..6126
                     /locus_tag="VF240_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173675.1"
                     /translation="MSTDSADEVVVNASHARRVGGRRRVIAATGPRMPSTAGYMAVAV
                     AVATIVFFVVGIMLYANEDEAPWLLASLAGGGVLLLAAAARELVMRRAWSRYTRDMEL
                     EMRRRTSVPPRATTAGRAGVPSSVHASASALRKLQNRLSEAPASGASPEAHLEAYHLC
                     EQYLADAEEAIRSAGGGSDVRAALRAGQERVRTLQKGHLLTWARGEATRLTQEAQRRV
                     RLSDKIETAQRALGIIDEALRVYPSEPELHRSATAVRDFMASVKVGHWVEMAERAAFR
                     GRYGRAIARYRDALFYLSRAEMGEATRDAAAERINREIELLRARVATDERTTTVGADY
                     DIIVEGLAPESDGDGERGDAPGGEGQT"
     gene            6129..6350
                     /locus_tag="VF240_00140"
     CDS             6129..6350
                     /locus_tag="VF240_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173676.1"
                     /translation="MSHCPKCRSSRIRRGYRDTPLLLRAIGIYSLLCDNCNLLFKGFA
                     VPGTVPARGSSRRKKRRGEDKDQGVRDRP"
     gene            6485..7552
                     /locus_tag="VF240_00145"
     CDS             6485..7552
                     /locus_tag="VF240_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7173677.1"
                     /translation="MNKLFFRAAALCLVAASMAVGQDQQQGQQSSSAPSSGQQTGANS
                     GTLGMVSDAMGIKRYQLGPGDVVELRVYNDPTLTGKYTVDVDGNIDIPLVGTIPAKCR
                     TDLEMKEEVLKGLKKYLKNPSISLSVAERNSRPMATVFGAVRAPTRVQMNRRVRLIEL
                     LAVAGGATEQASADIQIYHTEQIMCPTAEDLIIMEAEKKTDDAIGVPFNFYNIADVKA
                     GKQDANPYIRPGDIIIVQEAFPIFVTGAVVQPSNLYLRNDMTLFRAIAQVGGLRKDAK
                     TDKIYIYRKSKGKPAEPLIVNFDAIRKNKAPDVLLEPYDIVEVRDASPWSASMLPKTL
                     LGIATGAAQNVVSAGSVRIIN"
     gene            7718..8035
                     /locus_tag="VF240_00150"
     CDS             7718..8035
                     /locus_tag="VF240_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173678.1"
                     /translation="MDSRSRAGVASGKSRRRKTIVITLAVVAVVIALIAYEQVALLYV
                     ISTLSVVALLFIVAWSNLEGARRPAGAAPLDDSAAIGSGIAAAAPASNPRGTAARAGR
                     TRR"
     gene            8384..10489
                     /locus_tag="VF240_00155"
     CDS             8384..10489
                     /locus_tag="VF240_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEX7173679.1"
                     /translation="MRTLLTLVLLTGLLALPAAPARSYTLQYTALGAALQVKWPTTTI
                     NIALSTSLNTPPANIEATPAEVVAAARRALKTWSEAANIQFVITTSGAEALDPNDNIS
                     LITVSQVNATQLSCSPMQQGRARVSFDSVTGNIIEGDVAINPNLCARFSTDGRDGTFD
                     LESTLVHELGHVLGLEHTGVVGASMQPRQGTNGFYGLPALATRTLAEDDRTGLRAIYG
                     PRAGTGSIAGVVNYASGAPAFGAHVWAESLTTGEVVASNIALPTGAYRIDALPAGSYR
                     VLAEHLNEPVDASQIASRSGAYQGLANGTAPFIAREVGNFDVADGAVTQLSFTVSGSP
                     LLNPAWIGTGGQLSKIAAPLVPGRTTNILVGGDGITDGNFSILATPSINSPHMTVSNV
                     QSQGFGFGIPVISFDVTVGAAAPPGEYSLRLQNGAGEIAFVTGGLIIDLPAGVTTGNP
                     IDDSTFFVAQHYRDFLNREPDAAGLQFWTNQITSCGADAACREVKRVNVSAAFFLSIE
                     FQETGFLVYRVHTAAFATRERLRFRDFLLDQQRVSRGVVIGTPGAEALLEANTLAYLN
                     EFVARPQFLALYPTTLSAAQFVDALNRNANGALSADERNTLVNELTNSTKNRAQVLRA
                     VAADAELRGAEFNPAFVLTEYFGYMRRNPDDAPEPGLNFNGYNFWLTKLNGFGGNYIS
                     AEMVKAFITSGEYRQRFGL"
     gene            complement(10695..12842)
                     /locus_tag="VF240_00160"
     CDS             complement(10695..12842)
                     /locus_tag="VF240_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7173680.1"
                     /translation="MLRRTTSMLTVISLLTISLAPLTPEASAKKKADKGAKADSKLAP
                     EFATQAGGSQRIIVQTNGNREAAKSAVAAQGGSVRQDFGALDSFVAVVPASALGALAA
                     REDVHYVSPDRPVAASMNLTREATGASIVQAGSGNNGRNSLTGKGVTIAVIDSGVSAG
                     HPDFQGTNGKSRVLAAVDFTSTGSAQADGILAGDGILAGDGILMGDGVSNADKAGHGT
                     GVAGIIAGNGAASAGYAGSYAGIAPEASIIDLKALDGNGVGSTSAVMSAVNWVINNRA
                     RYQIRAINMSVGATVRESYKTDPLCQTVARAVRAGIVVVASAGNLGRFETGKDAAGQP
                     IYRTVYGSIKSPGNSPYVITVGASDTNGTAWRSDDRMAGFSSNGPTQFDHLAKPDLLA
                     PGRGVVAPMSQTADTYSADQRPDRVVSPASPGAQRDAYYKYYGTSFSAPMVTGTVALM
                     LERNKTLSPVLVKAALLRTAQGLGGEVWFDKMQAQLRQGAGQLNVAAAVEMAGAIVPN
                     ADKLRAGDQVFNHNVALSQLGQSVRIGGELVPAATRVLYSGGVLFNEHPILLNGILMG
                     DGILMGDGIMMSDGFVQGNGILMGDGILMGDGILAGDGILMGDGIMMSDGILMGDGIL
                     MGDGILMGDGILMGDGTTLDGIMMSDGIMMSDGIMMSDGIMMSDGILMGDGITLDGIM
                     MSDGILMGDGILMGDGILAGDGILMGDGILMGD"
BASE COUNT         2431 a         4420 c         4442 g         2178 t
ORIGIN      
        1 gaactgtacg tattggagcc acgcgcgatg ggagtgtgtt cgtagctatt gcgcgggcgg
       61 cagcggcggc gctgtcgcat ttcggggaca cgggaaacga aaatgtcaca cccggcatcc
      121 cacctctgcc accacgaaac gcgttcacgc ggcggaaccc ttaaccttgc cggcacaatt
      181 gtcaaagatt tgtcccgaga cttttacggc aagaagtccg agcacgtgcg ttcaaacgta
      241 taggtgggtg tggcccccgg tttgcttgat actgcggcaa ccgccggcgc cacacgataa
      301 gcggacgccc ggcgccacgc aaaataaagg aactttcatt caggtttctt cgtctaacga
      361 gagaccaaca acgaacaagg tgggtaagga aactacaatg aatacgaaga ccccgatcaa
      421 gatcatcaag agcggcgagc gcaaacggca ggcggaagcc tcggcccagg tagctgccac
      481 cgcggagaag aagacgccgc aggaagcggc gcgcgacatg gtcgcgaccg tcactgactg
      541 ggtgaacgag ttccagcaga agcgccgcgt tgagacgcgg caggcgctca ggaacttcct
      601 cacggagacg acgccccaga ccagttaaag tccggccaca cccgacgccg acacccggcg
      661 tctaactctt ccaacagctt cggagcgcga accgccgccg ccgcccggcc cccgtcatac
      721 ggaggcaagg gaggtgcggc gttcggcgct tcaccggctc gccaaatccc attgacaccg
      781 cgcggcgcac acgcatagca tcacgggcaa gttccaccca ttagacgaca cacgaggggg
      841 tgctctcgcg atgaccaaga cccgcaagac acagcccaag ggacgcgagc cgcggccggt
      901 gttcgacgcg atacgcaagc ccgccgcgcc gcccggtcac ccgctcacgc gcgccaagcc
      961 ggaggagcgc gcacacccgg ccgaacgcaa ggccaaacac aagcgcagcc ccgacgaacc
     1021 ggagtaagcc ggagggcacg gccccgcgcg cgcgtcggcc gcgaaacctc tgccggtacg
     1081 gactcaaaat aaagatggac gagatcacgc gtgagttcct gacggtcgcg cgcaagtacc
     1141 tgcgcgaaga ctacctgccg aagatcgagc gttgcctgga gcgcctgacg gacgaacagg
     1201 tctggtggcg cgctcacgaa acttccaaca gcatcggcaa cctcctgctc catctcgaag
     1261 gcaacgcgcg gcagtggatt ctgtgcggtg tcggcggggc gcgggacgcg cgcgtgcgcc
     1321 agagggaatt cgacgagcgc gcacagttgc cgcgcgccgt acttgtcgag aagctgaggg
     1381 cgacgctcga cgaagtggac ggcgtcctcg ccagtctcac cgcgcgggag ttcgacggag
     1441 gccgaagaag cattcagggc aacgacgtgt cggtgcttga agccgtcttc cacgtcgtcg
     1501 aacacttctc gatgcacacc ggacagatta tcttgctgac gaagacgctg accgcaagcg
     1561 acctcgcctt ctacgacact tcaggcggcg acccgcggcc ggactggcga cgcgcgactc
     1621 ccgacgacga ctaatcgcct ccgcataatt tttcgcttcc atatcgcgac gtttcgtgat
     1681 taatatacgt ctcgtaagtc gcatctcttg atcgccgacg ctcgggcgca tgtgaatcgg
     1741 agaagggtcg ccgccgagaa agcgcacgcg tcgccgccgt catacacagc ttaaggaggc
     1801 taacccgatg acatacacgg ttacggaccc ctgcgtgaac tgccgctaca cggactgcgc
     1861 tctggtctgt cccgtcgaag cgttccacct cgttgacgag ccagaccgca agttgctcgt
     1921 catcaacccg gagacctgca tcgactgcga cgcctgcgtc cccgagtgcc cggtcgaagc
     1981 catcttcccc gaggacaagg tgccggaaga tcaacaacac tggacgccgc tcaacgccga
     2041 gctgtgcatt ggcaatccca tccagacgga gaagatggaa gcggtctgct aggacgcgtg
     2101 cggcgggcgc tgtagcccgc cggcgcggag gcggtgacag aagtcggcgc gcaagcttcg
     2161 cccgacgcgg cggcggggct tgcgcattct tttgtccgaa aggttttcga gagcttgtca
     2221 aaactgagga cgaagaagag acggcggcgc ggtggtcgcc tgctgggtgc gctggggcta
     2281 gtggccggcg cggtcggcac gacgttcctg ctccgccgcc ggggcgagag gaaagcacag
     2341 ggagggctga ccaccgacga ccatgcgccg cgctctacag cggccgaccg ctgggcgcgc
     2401 cccggtatgg aggcgacctt ccgcgccgag ctgatgccgg gtcgcagtcg caccgagcgc
     2461 accttccgcg tcgcgcgtct cctgccgagc gggcgcgtca cgctcgacgg cgtcgcgggt
     2521 gaacacgccg agtcggagtt cgagcagata cgcaaatgac ccgcctgcct atcaaccaca
     2581 ggacagactc gccggtttaa gattccgaaa aaatgaagtg tcaggaagtg ctcggcgtcg
     2641 ccacgcgctc accacgagta gtcgcggccc gtctccatca cgctgaggcc ggggacgccg
     2701 agtgcgtcga gttcgcggac gattctttcg cggtaggccg gcttgatgtg catggcgaga
     2761 atttgagcgc cctgctgcgt gagcttggcg agttcgcggc cgagcgagtc gggcgtgagg
     2821 tggccggaga cttcggcgag gcgggcgagg gaatttggga aggatgcttc gacgaggagc
     2881 gcgtcgaggc ggggaaggcg gttgacgact tgccagaact cctcggtcgc cgccgtgtcg
     2941 gagctgaagg cgacggtcgc gcgcgtgtcc ttgaggatga ggccgaccgt cgggacgatg
     3001 tggttgacgg cgacggccgt cacgcgcagg tgggcgacgg cgaactcctc gcccgcgcgg
     3061 aaggggacat attcgaggac gcgcgtccgg ccgttgtcga aatcgtggaa gggcgggtaa
     3121 atcgtcccgt tgaaaacgtc gcggcgcagc agtcggacga cctcctcggt cgcgtggacg
     3181 cgcaccggcg tttcgagcaa ggcgaagttg tcatcgacga agatgggaag cgtcgcgatg
     3241 tgatccatgt gcgggtgcgt gacgatgaca tcacgcaccc gccggcagcg gtcgccgttc
     3301 aaggcgatgc tcccgccgtc gatggccaca cagtcatcga cgaggaagca gctcaggcgc
     3361 tgctcggggt tcgcgccccc ggcgcggtcg aagctgctcg gcagtagatg aatcttcact
     3421 cgcccgcccc cattctcatc ccgaaagtgt cagaagtctt atcccgaaag tgtcagggtt
     3481 aaccactcag cctcccgcgc agctcgacga gcgattgcag gtagtgcgtc tcgcgctcga
     3541 cggcagccga gtcgcgcggc gtccaaccgc gggcgacgcg gtggcggtga aaggccagct
     3601 cgctcgccac cccgtggagt cgcaggcggt cgcgccacgc cgaaaagcct ccgccgaaca
     3661 gcgcgccgag agaagcgccg aggcggcggc gcacggagca cagccgctca tactccacgc
     3721 gcgccagtgt gcctcgctcc acgtcgcccg ccagatgctc acgcaggatg cgcccctcct
     3781 tacgcagacc gaagaaggcg accatgagca cgcccgtaaa caccggcacc atgacgagga
     3841 agtacatcac cagcgcgatg agtccgcgct ccagcttgtc gctgaagaag agcgaggtgt
     3901 tccagacgct gtggagcacg acggccagcg ccagcccgac gaagggcagc gtcacgcgtg
     3961 cgctgcgccg ggcggcgtgg ctggccagcc ccagtccgat gccggtcatg ctcgtgaaca
     4021 acgggtgcgc gtaggggctg aacatcccgc gcacgacgaa ggtgccgagg gcgcgtcctt
     4081 cgagcgccgc ctcggcgtag tacttgatgt tctccgtcat ggcgaacccg aggccgacca
     4141 tcgaagcgta gacgatgccg tcgagcacgc cgtcgaactc gtcacgcttc cagaagtaga
     4201 ggatgaagag ggcgagcgcc ttcgtccctt cctcgacgag cggcgccgac agcgccagcc
     4261 cgtagaggtc ggccgcttcc tcgccgaagg ccgcatcgac ggcgaccatg ccgagggtgt
     4321 tgacgatgag cgcgacgccg acggcgacga gcgcgcccca gaagaacgcg gtggcgagca
     4381 gccagcccgg ctccgactcg aagcggtcga tccagagcac cagcagaacg tagacgggcg
     4441 cgggcagggc ggcgacgacg aggcccgtca ggaaaggaac aggccccgtc gagaaaccga
     4501 tcaggagcag cgtgacgagg ccgatgaggg cggcgaggag cacggccgtc gcggcgacca
     4561 cccacctgaa ggcgctcccc gatctcgggc gcgtcgcggc gcgagggccg ggcgcgagcg
     4621 cgggattgta gccgaggcgt gcggggtcgc tcatgggtct tcgtctcgcg ctcgcgccgc
     4681 ggggcggtcg cttctcccga acttgaaact cttccgttgg atggcgggcg agggcgggcg
     4741 agtcgggcac ggggccgcgt cgccggagtc ggtcgaaagt ggtagaccgg gccaagtata
     4801 ttcgcacggc gggggcgggt gccagataat ttttcccgcg gcggcgggcg gcgcgcgcta
     4861 cggtcgccgc tgcacgttga cagaccgggc cgaaaaattt gagtataatg acccgaccga
     4921 aacggcgagc cgccgctgat gggccgcgcg ccgcgcgagt ccgcggcctc aacgtgaggc
     4981 ggcgcgggcg gccgtcatcg ccccggacag ttcccgaaga ttcctttcgc ggagagaagt
     5041 ttgtccactg attcagccga cgaagttgtg gtcaacgctt cgcacgcgcg acgcgtgggc
     5101 gggcggcggc gcgtgatcgc ggccacgggg ccgcgcatgc cctcgaccgc cggctacatg
     5161 gcggtggccg tggccgtcgc caccatcgtc ttcttcgtcg tcggtattat gctctatgcc
     5221 aacgaggacg aggccccgtg gcttctggcg tcgctggccg gcggcggcgt gttgcttttg
     5281 gcggcggcgg cgcgcgagtt ggtgatgcgt cgcgcgtggt cgcgctacac gcgcgacatg
     5341 gaactggaga tgcgccgccg cacgagtgtc ccgccgcgcg cgacgacggc gggacgcgcc
     5401 ggcgtccctt cgagcgtcca cgcgtcggcc tcggcattac gcaagcttca gaaccgcctg
     5461 tcggaggcgc cggcttcggg agcctcccct gaggcgcacc tcgaagcgta tcacctgtgt
     5521 gagcagtatc tggcggacgc ggaggaggcg atccgctcgg cgggcggcgg ctcggacgtg
     5581 cgcgcggccc tgcgcgccgg acaggagcgc gtccgcacgt tgcagaaggg gcacctgctg
     5641 acgtgggcgc gcggcgaggc cacgcggctg acgcaggagg cgcagcggcg cgtgcgtctg
     5701 tcggacaaga ttgagacggc gcaacgcgcg ctcggcatca tcgacgaggc gctacgcgtc
     5761 tacccctcgg agccggagtt gcaccgctcg gcgacggccg tgcgcgactt catggcttcc
     5821 gtcaaggtcg gtcactgggt ggagatggct gagcgcgcgg ccttccgcgg gcgttacggg
     5881 cgcgccatcg cccgttaccg cgacgccctc ttctatctgt cgcgcgccga gatgggcgag
     5941 gcgacgcgcg acgcggccgc tgagcgcatc aaccgcgaga tcgagctgct gcgcgcacgg
     6001 gtcgcgaccg acgagcgcac gacgaccgtc ggcgccgact atgacatcat cgtcgagggg
     6061 ctggcgccgg agtcggacgg cgacggggag cggggcgacg cgcccggcgg ggaagggcag
     6121 acttgatctt gagccattgc ccgaaatgcc ggagttcgcg catccggcgt ggctacaggg
     6181 acacccctct gctgctgcgg gcgattggta tttatagtct cctgtgcgac aattgcaacc
     6241 tgctgttcaa ggggtttgcg gtgccgggga cggtgccggc gcgcggcagc agccggcgga
     6301 agaagcgccg cggggaagat aaggatcagg gggtgcgcga ccgcccctag agcgtgcgcg
     6361 ctggtcgtcc ccgcactttt ccgggcagcc atccgccgtc cgccagacat ctactaacat
     6421 ctgccttcga cccggcccga cacgaaggtt cgtcaattca tcaaaggatt ttgttatgag
     6481 agtaatgaat aaacttttct tccgggcggc cgccctttgc ctcgtcgccg cttcgatggc
     6541 cgtgggccag gatcagcagc aggggcagca gtcgtcctcc gccccttcct cggggcagca
     6601 gacgggcgcg aactcgggca ccctcggcat ggtgtcggac gcgatgggca tcaagaggta
     6661 tcagctcggc cccggcgacg ttgtcgagct gcgcgtctac aacgacccga ccctgacggg
     6721 caagtacact gtagacgttg acggcaacat cgatatcccg ctcgtcggca ccatcccggc
     6781 caagtgccgc accgatctgg agatgaagga agaggtgctg aaggggctga agaagtatct
     6841 caaaaacccc tcgatcagcc tctccgtcgc cgagcgcaac agccgcccga tggccacggt
     6901 cttcggcgcg gtgcgcgccc cgacgcgcgt ccagatgaat cgccgcgtgc gtctgatcga
     6961 actgctcgcc gtcgccggcg gggccaccga gcaggccagc gccgacatcc agatttatca
     7021 caccgagcag atcatgtgtc cgaccgcgga agacctgatc atcatggagg ccgagaagaa
     7081 gaccgacgac gccatcggcg tgcccttcaa cttctataac atcgccgacg tgaaggccgg
     7141 caagcaggac gccaacccct acatccgccc cggcgacatc atcatcgttc aggaggcgtt
     7201 ccccatcttc gtcaccggcg ccgtcgtcca gccgagcaac ctctacctgc gaaacgacat
     7261 gacgctcttc cgcgccatcg cccaagtcgg cgggctgcgg aaggacgcga agaccgacaa
     7321 gatttatatc taccgcaaga gcaagggcaa gccggccgaa ccgctcatcg tcaacttcga
     7381 cgccatcaga aaaaacaagg cgcccgacgt actccttgag ccttacgaca tcgtcgaagt
     7441 gcgcgacgcc tcaccctgga gcgcgtccat gctgccgaag acgcttctcg gcatcgccac
     7501 cggcgccgcg cagaacgtcg tcagcgccgg ctcggtcagg atcatcaact aagggcgggg
     7561 cgcgagttcc atcacgtcgg ggccggccgc gtgtgcgacc ggcctttttc tttttcaccg
     7621 tcaaggacgc cgctggcgac tcgggccgat tggcccgtct gtggtaaatt accccttcgt
     7681 ctttcgccgt aataatcaat cgacaggagt tgaccgaatg gatagcagga gccgcgcggg
     7741 cgtagcctcg ggcaagagtc gtcgccgcaa gacaatcgtc atcacgctgg cggtcgtcgc
     7801 cgtcgtcatc gcgctaatcg cctacgagca ggtggccctg ctctacgtga tctcgacgct
     7861 gagcgtcgtg gcgctcctct tcatcgtcgc gtggtcgaac ctggagggcg cgcgccgccc
     7921 cgcgggcgct gcgccgctcg acgactcggc ggccatcggc agcgggatcg ccgccgccgc
     7981 ccccgcgagc aacccgcgcg gcacggccgc gcgcgccggc aggacgaggc gctagggtcg
     8041 gccgcacccc gctttcgact tcctacgctt gccgccgccg ctcacacgcg cgacacaaaa
     8101 catcatgccc gacacgtcag gccgcactcg tcttcaaggg taacgcgaag cgtccgcgaa
     8161 ggcccgcgcg ccgcgcccgt cgcgttcgtc acacaggccg gtcgccgcac tgctctcagg
     8221 agttctaaag cggcggccga cctttttgtt tggcgcgtcc cccccgccct tgctatgatt
     8281 cggaagtctc gcggcaaccg cgagagaccc aattcaccat tacaaacatt ccccgcggta
     8341 ataagttctc ggcggacggt ttttccgaat caagggaagg ttcatgcgta cactgttaac
     8401 cctcgttctc ctgaccggac tcctggccct gcccgccgcg cccgcgcgct cctacacact
     8461 ccagtacacg gcactgggcg ctgcccttca ggtcaagtgg ccgaccacga cgatcaacat
     8521 cgcgctctct acttcgctca acacgccgcc ggccaacatc gaggccacgc cggccgaagt
     8581 ggtcgccgcc gcgcgccgcg ctctcaagac gtggtcagag gccgcgaata ttcaattcgt
     8641 catcacgacc tcgggcgccg aggcgctcga ccccaacgac aacatcagcc tcatcaccgt
     8701 ctcacaggtc aacgccacgc agctcagttg ctccccgatg cagcaggggc gcgcccgcgt
     8761 gagtttcgac tccgtcactg gcaacattat cgagggcgac gtggccatca accccaacct
     8821 gtgcgcgcgc ttctcgaccg acgggcgcga cggcaccttc gacctcgaat cgaccctcgt
     8881 ccacgagctg ggccacgtcc tgggtctgga gcacacgggc gtggtgggcg cgtcgatgca
     8941 gccgcggcag gggacgaacg gattttacgg cctgcccgcg ctggcgactc gcacgctcgc
     9001 ggaggacgac cgaacggggc tgcgcgccat ctacgggccg cgcgccggga cgggttcgat
     9061 tgcgggcgtg gtcaactacg cgagcggcgc cccggccttc ggcgcgcacg tctgggccga
     9121 gagcctgacg acgggcgagg tcgtcgccag caacatcgcg ctgcccaccg gcgcttaccg
     9181 catcgacgcc ctgccggccg gctcctaccg agtgcttgcg gagcacctca acgagccggt
     9241 ggacgcgtcc cagatcgctt cgcgctcggg cgcttatcag ggactggcga acggcacggc
     9301 tccgttcatc gcgagggagg tcggcaactt cgacgtggcc gacggcgccg tcacgcagct
     9361 ctccttcacc gtgtcgggtt cacccttgct caacccggcg tggatcggca cgggcgggca
     9421 actctcgaag attgccgcgc cgctcgtgcc gggccgcacg acgaacattt tagtcggcgg
     9481 cgacggcatc accgatggga acttcagcat cctcgcaaca cccagcataa actcgccgca
     9541 catgaccgtg tcgaacgtac agagtcaggg cttcggcttc ggcattcccg tcatcagttt
     9601 cgacgtgacg gtcggcgcgg cggccccgcc cggcgagtac agcctgcggc tccagaacgg
     9661 ggcgggcgag atcgccttcg tcacgggcgg actgatcatc gacctgcccg ccggcgtgac
     9721 cacgggcaac cccatcgacg actcgacctt cttcgtcgcg caacactacc gcgacttcct
     9781 caaccgcgag ccggacgccg cgggcctcca gttctggact aatcagatca cctcctgcgg
     9841 cgccgacgcc gcctgccgtg aagtcaaacg cgtcaacgtc tcggccgcct tcttcctctc
     9901 catcgagttt caggagaccg gcttcctcgt ctaccgcgta cacaccgccg ccttcgccac
     9961 acgggagcga ctgcgcttcc gagacttcct gctcgaccag cagcgggtca gccggggcgt
    10021 ggtaatcggg acgcccggcg ccgaggcgtt gcttgaggcg aacacgctgg cgtacctgaa
    10081 cgagtttgtc gcgcggcccc agttcctcgc gctctacccg acgacgcttt cggccgcgca
    10141 gttcgttgac gcgctcaacc ggaacgcaaa cggcgcgctg tcggccgacg agcgcaacac
    10201 gctcgtcaat gaactgacca acagcacgaa aaaccgcgca caggtcttgc gggccgtggc
    10261 cgcggatgct gagctgcgcg gggccgaatt caacccggcc ttcgtcctga cggagtactt
    10321 cggctacatg cgacgcaacc ccgacgacgc gccggagccg ggcctcaact tcaacggcta
    10381 caacttctgg ctcacgaagc tcaacgggtt cggcggtaac tacatctcgg ccgagatggt
    10441 caaggccttc atcacgagcg gcgagtaccg gcagcgattc ggcctctaaa gccgtttttg
    10501 atttgccgta ggacaggcat tcctgcctgt catgtgaaat tcaagacagg cattcctgcc
    10561 tgtcctacat agactgacgc gacataacaa taaggcggga cgagttttga aaactcctcc
    10621 cgccttattt tatgtcgccc gcggacgctg cgtcttgacg accgcggcgg caaggcgaag
    10681 acccttcggc cgactcagtc gcccatgagg atgccgtcgc ccatgaggat gccgtcgccg
    10741 gccaggattc cgtcacccat caggataccg tcgcccatca gaatcccgtc gctcatcatg
    10801 atgccgtcga gcgtgatgcc gtcgcccatg aggatgccat cgctcatcat gatgccgtcg
    10861 ctcatcatga ttccatcgct catcataatg ccgtcgctca tcataatgcc gtcgagcgtc
    10921 gtgccgtcgc ccatcaggat tccgtcaccc atgaggatgc catcacccat gaggatgccg
    10981 tcgcccatca ggataccgtc gctcatcatg atgccgtcgc ccatgaggat gccatcgccg
    11041 gccaggatgc cgtcgcccat caggatgccg tcacccatca ggattccgtt accctgcacg
    11101 aagccgtcgc tcatcatgat gccgtcgccc atgaggatgc cgtcgcccat gaggatgccg
    11161 ttgaggagga tggggtgctc gttgaagagg acgccgccgg agtagagcac gcgcgtcgcg
    11221 gccgggacga gttcgccgcc gatgcggacg gactggccga gctggctgag ggcgacgttg
    11281 tggttgaaga cctgatcgcc ggcgcggagc ttgtcggcgt tgggcacgat ggcgccggcc
    11341 atctcgacgg ccgccgcgac gttcaactgg cccgcgccct gccggagctg cgcctgcatc
    11401 ttgtcgaacc acacttcgcc gccgagaccc tgcgccgtgc ggagcagcgc ggccttgacg
    11461 agcacggggg agagcgtctt gttgcgctca agcatcagcg caaccgtgcc cgtgaccatc
    11521 ggggcggaga acgacgtgcc gtagtacttg tagtaggcgt cgcgctgcgc gcccggcgag
    11581 gccggggaga cgacgcggtc ggggcgctgg tcggccgagt aggtgtcggc cgtctgcgac
    11641 atcggggcga cgacgccgcg gccgggcgcg agcaggtcgg gcttggcgag gtggtcgaac
    11701 tgcgtggggc cgttcgagga gaagccggcc atgcggtcgt cggagcgcca cgccgtgccg
    11761 ttggtgtcgg aagcgccgac ggtgatgacg taggggctgt tgccggggct tttgatggag
    11821 ccgtagacgg tgcggtagat gggctggccg gcggcgtcct tgcccgtctc gaagcggccg
    11881 aggttgccgg cgctggcgac gacgacgatg ccggcgcgga cggcgcgggc cacggtctgg
    11941 cagagcgggt cggtcttgta ggactcgcgg acggtggcgc cgacgctcat gttgatggcg
    12001 cgaatctggt agcgcgcgcg gttgttgatc acccagttga cggcgctcat cacggcgctc
    12061 gtggagccga cgccgttgcc gtcgagggcc ttgaggtcga tgatgctggc ttcgggagcg
    12121 atgccggcgt agctgcccgc gtagccggcc gaggccgcgc cgttgccggc gatgatgccc
    12181 gccacgcccg tgccgtgacc ggccttgtcg gcgttgctca cgccgtcgcc catcaggatg
    12241 ccgtcgcccg cgaggatgcc gtcaccggcg aggatgccgt cggcctgcgc gctgccggtc
    12301 gaggtgaaat cgacggcggc gaggacgcgg gatttgccgt tcgtgccctg aaagtcgggg
    12361 tggccggcgc tgacgccgct gtcgatgacg gcgatggtga cgcccttgcc cgtcaggctg
    12421 ttgcggccat tgttgcccga gccggcctgc acaatcgagg cgcccgtcgc ttcgcgcgtc
    12481 aggttcatcg aggcggccac ggggcggtcg ggcgaaacgt aatgcacgtc ttcacgcgcg
    12541 gccagagcac cgagggcgct cgccggaacc acggcgacga aggagtcgag cgcgccgaag
    12601 tcctgacgga cagagccgcc ctgcgcggcg actgcgctct tggcggcctc gcggttgccg
    12661 ttggtctgga cgatgatgcg ctgagagccg ccggcctgcg tcgcaaactc gggggcgagt
    12721 ttgctgtcgg ccttggcgcc cttgtcggct ttcttcttgg ccgaggcttc gggagtgagc
    12781 ggggcgagag agatggtgag gaggctgatg acggtcagca ttgaagtggt tcgacgcaac
    12841 atttcgtgtc ccttctttcc ggcgcctgat ttctgtctgc tgcccaagca gtggaggaag
    12901 gaatcggcgc cactcaaact caaaattttc aagtaaatcg ttgaaggagt ctcgagttct
    12961 gctcgtgagg cttgagagct aaaggccgct ttcactctcg aaccccgaaa ctcctgtcaa
    13021 cgatcttgca ctcccctatt acaacggacg tgccactaaa gagcaccgcc gaagggacac
    13081 cggcggacgc acttaaacct caaagcttga actgtttaca ggaacgaacg acgaaggcgg
    13141 gaaactcgca tgacggaagc caatttggat accaagtatc cacgcccaga gcagaacagc
    13201 cgactaatgc gggtatcttg caaaatcagc ggctaattca gaaaactaga cgagtgtgga
    13261 taagtgtgta ccgggtatgc agtgtgtacc cggtacacac ttaaaggctt tgcctcaaaa
    13321 gctcgtcttg cctcataagt cggcgggctg cctcaaaagt cattggttgg ccgccttgct
    13381 ctgtcttgcg gctgtgccgg ggaacggcat ccctttatcg cgcagcctct cgaaagtctt
    13441 gattgaccgc cgaaggtcgc cgatgtcttt c
//
LOCUS       DASVES010000005         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_187395, whole genome shotgun sequence.
ACCESSION   DASVES010000005 DASVES010000000
VERSION     DASVES010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187395"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VF240_00165"
     CDS             <1..263
                     /locus_tag="VF240_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173681.1"
                     /translation="DVPMLLVPRFTVGDYTHPHLAVAVLDMAPINETAGFEQTGIIGG
                     NILRFFRVTFDFGRGVVRMEMIPGYVPPVAQPREATVTPQGY"
     gene            269..754
                     /locus_tag="VF240_00170"
     CDS             269..754
                     /locus_tag="VF240_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7173682.1"
                     /translation="MKQSLYLETSVVGAYLDNGEPFRRDLTIRWWEREMSEYRAVVSP
                     LVARELERVPEPHRTGYLKLIEPLERVELSDEATILAEGYVSRGIFHRKYIGDALHAA
                     IASVHKIDYLVTWNFGHLANVRRQARIRLFNTAAGFFVPQIVTPEFLVSESTEKVIGD
                     K"
     gene            823..1059
                     /locus_tag="VF240_00175"
     CDS             823..1059
                     /locus_tag="VF240_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173683.1"
                     /translation="MYRKPKFLEVLHAIREEMSREADYDVELFAEMVRSGVRPRRGPA
                     RNIRGVRTHAPAAGEEQRDESPPRPVRQRKRAAR"
     gene            1153..1944
                     /locus_tag="VF240_00180"
     CDS             1153..1944
                     /locus_tag="VF240_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173684.1"
                     /translation="MEETFEAIGLDVPSERDFNRLAERAGDQGEPTRLPRRGAVLHGR
                     CWKLGEGLEVWTVLYESETGEIFYADCRPGFRARYTQRVSPWALTEYDEDGEAIVHGY
                     CDGTDTEVLFELQNLTEVGAAGFGAQELYVGLCGLAYRAEVSDAGVAPARWQPLDAVA
                     PARATHENDWSLRGRVISFKPLRNPLTGGELYWVYLDLERFKLEVLVNQRALSGGRLF
                     VGAQLSAEVWLQGHLLDQRVLRSRYEGVDTSSTVFDHWSHLKRRN"
     gene            2037..2618
                     /locus_tag="VF240_00185"
     CDS             2037..2618
                     /locus_tag="VF240_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7173685.1"
                     /translation="MFKRLVPVAIALLFLAGAGFYLFNRYWIHRYDEMIARQASVYRL
                     DPELVWSIIHEETYFSPWKVGEDGEIGLMQVTPTVGREWAAETGMRELEQQMRGDPAA
                     ALRDPERNIQVGCWYLEKISKDYRDTPGAEARVVAGYNAGPSRAQEWNRTERGAPPLT
                     AEEFIARIDIPSTRAYVSNIIARYRAKKVSSKQ"
     gene            2765..>3170
                     /locus_tag="VF240_00190"
     CDS             2765..>3170
                     /locus_tag="VF240_00190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7173686.1"
                     /translation="MTYRQLLAGNADLRRLWMGQAVSEVGDWLNNISVLALVIELAGG
                     GRVGLAIAVYAIARHLPLFVFGPVAGVVVDRLSRRRVMIAADLLRAALALGFLLAASG
                     RSLPIIYAVGASLFAVSAFFNAAKRAAVPNLAR"
BASE COUNT          533 a         1101 c          995 g          541 t
ORIGIN      
        1 acgacgtgcc gatgctgctc gtgccgcgct tcacggtcgg cgactacacg cacccgcacc
       61 tcgccgtcgc ggtgctcgac atggccccga tcaacgagac cgccggcttc gagcagacgg
      121 ggattatcgg cggcaacatc ctgcggttct tccgcgtcac cttcgacttc gggcgcggcg
      181 tcgtccgcat ggagatgatc cccggctacg tcccgcccgt cgcccagccg cgcgaggcga
      241 ccgtcacgcc gcaggggtac tgaacgggat gaagcagagc ctctaccttg agacctcggt
      301 cgtcggcgcg tacctcgaca acggcgagcc gttccgccgc gacctcacga tccgctggtg
      361 ggagcgcgag atgtcggagt accgcgcggt cgtctcgccg ctcgtcgcgc gcgagctgga
      421 gcgcgtgccg gagccgcacc gcacgggcta cctcaaactg atcgagccgc tcgaaagggt
      481 cgagctctcc gacgaggcga ccatcctcgc ggaagggtac gtctcgcgcg gcatcttcca
      541 ccgcaagtac atcggcgacg ccctgcacgc cgccatcgcg tcggtgcaca agatcgacta
      601 cctcgtgacg tggaacttcg gccacctcgc caacgtccgc cgacaggcgc gcattcgtct
      661 cttcaacacg gccgccggct tcttcgtccc gcagatcgtc acgccggaat tcctcgtctc
      721 ggagtctact gaaaaagtga tcggtgataa gtgatgagtg ataagatttg agtgttggct
      781 ctttttctta taacttatca cttgtcactt atcacggttc ttatgtatcg caagccgaaa
      841 tttctggaag tgcttcacgc catccgcgag gagatgtcgc gtgaggccga ttacgacgtg
      901 gagctgttcg ccgagatggt gcgctccggc gtgcgcccac gccgcggccc cgcgcgcaac
      961 atccgcggtg tgcgcacgca cgcccccgcc gccggcgagg agcaacggga cgagtccccg
     1021 ccccggccgg tcaggcaacg caagcgcgcc gcgaggtgag cgcgtcgccg tcgccggctg
     1081 cttccggcat tagcgtgcga aaagaaaatt gctttactga cctctaacct ctaacctctg
     1141 acctctgctt ttatggaaga gacgtttgaa gccatcgggc tggatgtgcc gagcgagagg
     1201 gacttcaacc ggctcgccga gcgcgcgggc gatcagggcg agccgacgcg cctgccgcgc
     1261 cgcggcgccg tgctgcacgg ccgctgctgg aagctcggcg agggcctcga agtctggacg
     1321 gtgctctacg agtcggagac gggcgaaatc ttctacgccg actgccgccc cggctttcgc
     1381 gcccgctata cgcagcgcgt cagcccgtgg gcgctcaccg agtacgacga agacggcgag
     1441 gccatcgtcc acggctactg cgacggcacc gacaccgagg tgcttttcga gttgcagaat
     1501 ctgaccgagg tcggcgccgc cggcttcggc gcgcaggaac tctacgtggg cctgtgcggg
     1561 ctggcttacc gcgcggaggt gtcggacgcg ggcgtcgcgc cggcgcgctg gcagccgctc
     1621 gacgcggtcg cgcccgcgcg cgccacgcac gagaacgact ggagcctgcg gggccgcgtc
     1681 atctccttca agcccctgcg taaccccctg acgggcggcg aactctactg ggtctatctc
     1741 gacctcgaac gcttcaagct cgaagtcctc gtcaaccagc gcgcgctcag cggcggccgg
     1801 ctctttgtcg gcgcgcagct ctcggccgaa gtctggcttc agggccacct cctcgaccag
     1861 cgcgtgctgc gctctcgtta cgagggcgtg gacacctcct cgaccgtctt cgaccactgg
     1921 tctcacctga agcggaggaa ttgaacgaca gaggtcagag gtcagaggtc agaggatagt
     1981 aaaaaccaat ccatctttca ctgacctcta acctctaacc tctgacctct gtttttatgt
     2041 tcaagcgtct cgtccccgtc gccatcgccc tcctgtttct cgcgggcgcc ggcttctacc
     2101 tcttcaatcg ctactggatt caccgctacg acgagatgat cgcccggcag gcgtccgtct
     2161 atcgactcga ccccgaactc gtgtggagca tcatccacga ggagacctac ttcagcccgt
     2221 ggaaggtggg cgaggacggc gagatcggtc tgatgcaggt gacgccgacg gtgggccgcg
     2281 aatgggccgc cgagaccggc atgcgcgagc ttgaacaaca gatgcggggc gacccggccg
     2341 cggccctgcg cgaccccgag cgcaacattc aggtcggctg ctggtatctg gaaaaaatct
     2401 cgaaggacta ccgcgacacg cccggcgccg aggcgcgcgt cgtcgccggc tacaacgccg
     2461 gccccagccg cgcgcaggag tggaaccgca ccgagcgcgg cgcgccgccg ctcaccgccg
     2521 aagagttcat cgcgcgcatc gacatcccct cgacgcgcgc ctacgtctcg aacatcatcg
     2581 cgcgctaccg cgcgaagaaa gtaagcagta agcagtgagc agtaagcagt gaaggcaagc
     2641 agtcaggagc gagcagaagg gcgccgcaga tgcgacacgt gaactcgaat aaggccagac
     2701 cggcccgcga caacttccta ctgctcactg ctcactgctt actgctcact ccccggaggg
     2761 gcctgtgacc tacaggcaac tgctcgcggg caacgccgac ctgcgccgcc tgtggatggg
     2821 gcaggcggtc tccgaggtcg gcgactggct gaacaacatc tcggtgctcg cgctcgttat
     2881 cgagctggcg ggcggcggcc gggtggggct ggcgattgcc gtctacgcca tcgcgcgcca
     2941 cctgccgctg ttcgtcttcg gcccggtcgc cggagtcgtc gtggatcgac tgagccggcg
     3001 gcgcgtgatg atcgcggccg acctcctgcg cgccgcgctc gccctcggct tcctcctcgc
     3061 cgcaagcggg cgttcgctcc ccatcatcta cgccgtcggc gcatctctct tcgccgtctc
     3121 agccttcttc aacgccgcca agcgcgccgc cgtcccgaac ctcgcgcgcg
//
LOCUS       DASVES010000006         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_187021, whole genome shotgun sequence.
ACCESSION   DASVES010000006 DASVES010000000
VERSION     DASVES010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187021"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(570..761)
                     /locus_tag="VF240_00195"
     CDS             complement(570..761)
                     /locus_tag="VF240_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173687.1"
                     /translation="MAKEAVSAGFHTSAWGNFPRVQILTIEDLLTGKATAHYPRLNAA
                     TFKRAERKRREQGEQSGLF"
     gene            complement(1031..2623)
                     /locus_tag="VF240_00200"
     CDS             complement(1031..2623)
                     /locus_tag="VF240_00200"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX7173688.1"
                     /translation="MNPRKIALTISVALACVLACVNVLAQATSTPFASGLKAPTKSVL
                     TGRGNLLVAEAGNGPNTGRLSLIDRNTGARRTILDGLPSGINALGGAPAPSGPSGLAL
                     AGDTLYMTISTGDSVEPGPNPGTEKESPAPSSPIMSSVLSIRASSNVETGTASFALTS
                     ADHTTLKNGGQVVLSTALGETLTIELVVDFPNFVAEPRPDFPANVRASNPFGVAVSGG
                     LLYVNDAGLNLVKTVGIFSHTSATLTTFAPIARTSMTPPGPPVMDAVPDSVRLYGDQL
                     LVTLLTGFPFEQGRSEVRKVDLVGGGQTTFIGGLSTAIDMHAIRRGSNPDHFLVLEFS
                     TNMLGGAPGRMLYFSSTAGPPFVFTSPLVSPTSISRDRRTGDVFITQIFPGLVARVDG
                     ARFFVRQHYLDFLGREPDIDGWDFWTNQIASCGNDEGCRDRKQVDVSRAFYYSGEFIA
                     AHPELAANLRGTTTYNRAFVKQAYLTYLQRTCDPEVCDAAGFNFWVNILNSKIPNTDA
                     HYNEMIRAFILSSEYRARLGLS"
     gene            complement(3016..3951)
                     /gene="mdh"
                     /locus_tag="VF240_00205"
     CDS             complement(3016..3951)
                     /gene="mdh"
                     /locus_tag="VF240_00205"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7173689.1"
                     /translation="MGKRNKITVVGAGNVGATAAHWLASKELGDVVLVDIIEGMPQGK
                     ALDLAQAAPVEGFDARLTGATNDYAQTKDSDVVIITAGLPRKPGMSRDDLLKTNAEIV
                     SNVVDQVVRYSPESVLIIVSNPLDAMCQVAFKRSGFPKHRVIGMAGVLDSARMRCFLA
                     EALDVSVENVTAFVLGGHGDTMVPLPRYSTCAGIPVTELLPKDQLDAIVKRTANGGAE
                     IVGLLKTGSAYYAPSAAAVEMAEAILKDKKKILPCAAYLEGEYGVQGLYVGVPCKLGA
                     RGLEQIIEINLTAEERIALQKSAASVQELVTVLGM"
BASE COUNT          705 a         1186 c         1278 g          835 t
ORIGIN      
        1 atcaggggcg gcgactcttt tgtcgtcaat tcgattgtgc cagttgtgaa tctatgtgaa
       61 cggagggtgg gcgactccgg tatcaaagag tcgcaaattc gccttcacta attcctgctt
      121 gacgcaggat gctccggagc gtgccggcgg cgatttcttt gtgcttcggg acaactgccg
      181 tgcgtgtccc ttcggcggta atccgtgcga atttaatgtg actgccactc tgccctgctt
      241 ccgcaaagcc cgctgcttcc agcttgcgtt tgacttcgcg aaaagggagc ggcttcacgc
      301 ggctacctca acctcaacct gtcggacttg cggcgcaatt gtggcgacgg ggggcgcaaa
      361 gtgtaaccct aaagcgtcgc ttagattgtc taaggcttct gtttcggttg cgccctgtga
      421 agcaacatca acttcaaggc attgagcgat aaaccacgcg ccttcctgcc aaactgtcgc
      481 attaaagctt cgtttcattg ttgctcctcc tcctaagaac caactactca tgggatttta
      541 gcacgcttaa ttggacacga aacaagttgc taaaataatc cgctttgctc gccttgttcg
      601 cgtcgtttgc gttcggcgcg tttgaaggtg gcggcgttga ggcgcgggta gtgcgcggtc
      661 gccttgccgg tgaggaggtc ttcaatcgtg aggatttgaa cacgcgggaa gttgccccac
      721 gcggacgtgt ggaagccggc gctgactgct tctttagcca tgtctctggt cggcggctgg
      781 cgcccgtctc gcatgagctt gacgggcaca cgtgctcctc agttatgggg tttgcttcgg
      841 cggttcttcg gcccgcttgc gcgtcagtcc ccgcttgcgg tcgctcttgc gggtgtagcc
      901 gaaggcttcg tttagctcaa cagaataagt caaaggtaaa aaggatgaag acacaggtga
      961 gggtgattgc tcccctcgcc tgtgtcttct cttttccctg tccctgtcgg cctttgcctt
     1021 ttcctattac ttaactcagc ccgagccgcg cgcggtactc gctggagagg atgaaggcgc
     1081 ggatcatctc gttgtagtgg gcgtccgtgt tgggtatctt gctgttgagg atgttgaccc
     1141 agaagttgaa gcccgcggca tcgcagacct ccgggtcgca cgtccgctgc aagtacgtca
     1201 ggtaagcctg cttgacgaag gcgcggttgt aagtggtcgt cccgcgcagg ttggcggcca
     1261 gctccgggtg ggccgcgatg aactcgcccg agtagtagaa ggcccgcgac acgtccacct
     1321 gcttgcggtc gcgacagcct tcgtcgttgc cgcaagaggc gatctgattc gtccagaagt
     1381 cccagccgtc gatgtcaggc tcgcggccga ggaagtcgag gtagtgctgg cggacgaaga
     1441 agcgcgcgcc gtcaacgcga gcgaccagtc cggggaagat ttgggtgatg aagacatcgc
     1501 ccgtccgccg gtcgcggctg atgctggtcg gtgacacgag cgggctggtg aagacgaacg
     1561 gaggccccgc cgtcgaactg aagtacagca tccggcccgg cgcgcccccg agcatgttgg
     1621 tgctgaattc aaggacgaga aaatggtcgg gattgcttcc acgtctgatg gcgtgcatgt
     1681 caatcgccgt gctcagtccg ccgatgaacg tcgtttggcc gcccccgacg agatcaacct
     1741 tgcgaacctc cgagcgtccc tgctcgaacg ggaagcccgt cagcagcgtc accagcagtt
     1801 ggtcgccata gagccggacg ctgtcaggga cggcgtccat cacgggcggg ccggggggag
     1861 tcatgctagt cctcgctatc ggcgcgaagg tcgtcagggt cgcgctcgtg tgcgaaaaga
     1921 tgccgaccgt cttgacgagg ttcaggcccg catcgttgac gtagagcaag ccccctgaca
     1981 cggcgacgcc gaacgggttc gaggcgcgca cattggccgg aaagtcgggc ctcggttccg
     2041 cgacgaagtt tgggaaatcg acgaccagct cgatggtcag cgtctcgccc agcgcggtgc
     2101 tgagcaccac ctgtccgccg ttcttgagcg tcgtgtggtc ggcgctcgtg agcgcgaagc
     2161 tggccgtgcc ggtctcgacg ttggagctgg cgcggattga aagcaccgaa ctcatgatcg
     2221 gcgacgaagg cgcgggactc tccttctccg tgccggggtt ggggccgggt tcgaccgagt
     2281 cgcccgtcga aatggtcatg tagagcgtgt cgccggcgag cgccagcccc gaaggcccgg
     2341 acggggcggg tgctccgccg agggcgttga tccctgacgg aagaccgtcg aggatcgtgc
     2401 ggcgggcgcc ggtattgcgg tcgatcaggg aaagtcgtcc ggtgttgggg ccgttgcccg
     2461 cttcggcgac gagcaggttg ccccggcccg tcaggacgga cttcgtcggg gccttgaggc
     2521 cgctggcgaa cggggttgag gtggcctgag ccagcacgtt gacacacgcc agaacacacg
     2581 cgagggcaac ggagatagtc agcgcaatct ttctgggatt cattatcctc tcctttcagg
     2641 tcgagcgccg acatcgactt gcgaacgccg gcgagggttc tcctttaaac aattcctccc
     2701 ccttgaactt cgacgcaaac atcggggggc gaaaagtatt ccactaagtc ttattttgca
     2761 agagaaaact ttcgcggcgg ccgtcatttg tcctcattaa attacgcgag acccgcccgg
     2821 caaaacccgc acggtcaggg ataatatttt caactgaagg ggccggaagt gttgttgagt
     2881 ggtgatagaa tccgcggggc gagcctttga tcttcattct tgacgacaaa agacgcgcgg
     2941 gcgaagtgtt ctcttccggt tgtgaacact tcgcccgcgc gtcttttgtt actcatgcgc
     3001 ccaaccgtcg ggctgctaca tcccgagcac ggtgacgagc tcctgcaccg acgcggcgct
     3061 cttctggaga gcgatgcgct cctcggccgt gaggttgatc tcgataatct gttcgagacc
     3121 gcgcgcgccg agtttgcagg gcacgccgac gtagaggccc tgcacgccgt actcgccttc
     3181 gaggtaggcg gcgcacggca gaatcttctt cttgtccttg agtatcgctt cggccatctc
     3241 gaccgcggcg gccgacggcg cgtagtaggc cgagccggtc ttgagcagtc cgacgatctc
     3301 ggccccgccg ttggccgtgc gcttcacgat ggcgtcgagc tgatccttcg gcaaaagctc
     3361 cgtgacgggg atgccggcgc aggtcgagta gcgcggcagc ggcaccatcg tgtcgccgtg
     3421 accgccgagg acgaaggccg tgacgttctc cactgacacg tccagcgcct cggcgaggaa
     3481 gcagcgcatg cgcgccgaat ccaacacgcc ggccatgccg atgacgcggt gcttggggaa
     3541 gcccgaacgc ttgaaggcca cctgacacat cgcgtcgagc gggttcgaga cgatgatcag
     3601 gaccgactcc ggcgaatagc gcaccacctg atcgacgacg ttcgagacga tctcggcgtt
     3661 cgtcttcagg aggtcgtcgc ggctcatgcc gggcttgcgt ggcagcccgg ccgtgatgat
     3721 gaccacgtcg gagtctttgg tctgcgcgta gtcgttcgtc gcgcctgtga gccgcgcgtc
     3781 gaagccttcg acgggcgcgg cctgcgcgag atcgagcgcc ttgccctgcg gcatgccctc
     3841 gatgatgtcc acgagcacca cgtcgcccag ttccttgctc gccagccagt gcgcggcggt
     3901 cgcgccgaca ttgcccgcgc ccaccacagt gatcttgttt cgtttaccca tcgcttttca
     3961 tcctttcaaa ctagagacag aattttgccg gtggagatca caac
//
LOCUS       DASVES010000007        27871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1546036, whole genome shotgun sequence.
ACCESSION   DASVES010000007 DASVES010000000
VERSION     DASVES010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 27871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546036"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VF240_00210"
     CDS             <1..224
                     /locus_tag="VF240_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX7173690.1"
                     /translation="AVVRAREVGRDNWKKEAGYHRRSLAETAMMRLKTIFSDRLRARE
                     WRRQVTELRVRCVAMNRMTSLGMPQSYAV"
     gene            433..714
                     /locus_tag="VF240_00215"
     CDS             433..714
                     /locus_tag="VF240_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEX7173691.1"
                     /translation="MSQLLDRVRGAVRARHYSIRTEEAYLRWIREYILFFNKRHPSEL
                     GAPEVGAVVSHLAVQRNVAASTQKQALSALLFLYREVLDQPIEWVISGY"
     gene            complement(1029..2288)
                     /gene="hutI"
                     /locus_tag="VF240_00220"
     CDS             complement(1029..2288)
                     /gene="hutI"
                     /locus_tag="VF240_00220"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX7173692.1"
                     /translation="MSETFVVVNCSQLVTLRGPARPRVGAELRELSIIEDGAMLVSGE
                     RVARVGTRAEIEPLIERDAEVFDAGGRVVAPGFVDAHAHPVFAGNRADEFERRAEGAT
                     YQEIAAAGGGIRATVRRTRAASEDELFAESKRRAQWFLRTGTTTVEAKSGYGLTVEDE
                     LKMLRVVRRLGEETPLRFVPTFLGAHEVPEEHRADRAEYVRLVIEEMLPRVAGEGLAE
                     FCDVFCEGRVFGVDESREILLAARAHGLGLRVHADQLSLSGGARLAAELGAATADHLE
                     HADAAGIEALRAAGVQPVLLPGSVYALGSRQYPPARQMIERGLSVVVATDFNPGSSPT
                     PSMTMVLSLASTHMKMTPAEGLSAATVNAAHSLRRGRDLGTLEEGKLADFVVHDAHDY
                     RELAYFFGVEHAWAVFIAGRLVYRRGA"
     gene            complement(2281..2613)
                     /locus_tag="VF240_00225"
     CDS             complement(2281..2613)
                     /locus_tag="VF240_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173693.1"
                     /translation="MIKKTWKRWLLSAVLAAVVMFADTVALAQLNKWYRPGHPPAWAS
                     GFFYYLLAWPLPLTRLVFPRAAGDADEGPSFAAVASAGLIDLLLLTLLVYALLSRRAR
                     RKERAASV"
     gene            complement(2725..3201)
                     /locus_tag="VF240_00230"
     CDS             complement(2725..3201)
                     /locus_tag="VF240_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX7173694.1"
                     /translation="MKRRLSLGLAASLAASAAALYSACANVSEVSDKEGEQAVTAVRK
                     VIEEQQAAWNRGDIEGFMDGYAREETTTFVSGDAVTRGWQTVLDRYKRTYDTREKMGT
                     LEFTELEFRPVSPFYVAAVGRWHLTGVSGDPRGRFTLLFRRTAAGWRIVHDHTSSA"
     gene            complement(3276..3980)
                     /locus_tag="VF240_00235"
     CDS             complement(3276..3980)
                     /locus_tag="VF240_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7173695.1"
                     /translation="MMNQTEREPLQTERTLTAEIKLYYDLHAPAQTPAPLLVALHGYG
                     DNKGWMMRVAREVAPEGFAVASLQGPHQHIKEARVSGGPLRYGFGWLTNFRPEESVAL
                     HHRALLDLVDALDRQSVVRPDAVFLLGFSQTCALNFRFAFTHAERLRGVIGVCGGLPG
                     DWEESTLYRRTRASVLYLHGARDEFYPPERVADYAARLRARAADVETKSYDAGHEFTS
                     AMRLDVRAWLAARAGV"
     gene            complement(4045..6165)
                     /locus_tag="VF240_00240"
     CDS             complement(4045..6165)
                     /locus_tag="VF240_00240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX7173696.1"
                     /translation="MKRYVIKRPAGAGLPDRLARYREELNAEQLAVATAPPGAALVIA
                     GAGSGKTRAITYRVAYLVEQGVSPARIMLATFTNRAAREMLRRVEQLAGAQADAARRV
                     WGGTFHRIANLCLRRHAESIGYTANYTILDSEDARDLLSACIVEAGVDTRARRFPKAE
                     VLQDVISFATNTDQPIEDTIARRHPYFEPLTAAVKQVDRVYMERKRERNVMDYDDLLL
                     NWKRLLEEKDEVARLYQEQFEHILVDEYQDTNKLQAEIVDLLGVKHRGVMVVGDDAQS
                     IYAWRGAEWANIYEFPKRYPEARTFRLETNYRSTPEILAVANVSIAANRKQFEKTLRA
                     ARPSLGLPPALVPCRDADQQAAFVASRVLELRDEGVPLEEIAILYRSHYHSLELQLEL
                     TRRGIPYAVRSGVRFFEQAHIKDVVSYLRLVVNPRDELAWKRVLRLVPNVGQATAARV
                     WERLAYAPDPLALVVRGGADEAVRTRGTGWTEFRALVESLLADDTRATPARQIELILA
                     RGYESYLAANYENAETRLEDLRQLALYAARYNSTEDFLGELALLSTERYGGPQPLTGE
                     DVVSGGEEDELLTLSSVHQAKGLEWRVVFLIWASDGRFPSPRSLRDAEGEEEERRLWY
                     VAITRARDQLYIAYPLTATDHTRQTLLQRPSRFVTEVPPDLFEVWSLEEDNALSLGPA
                     TQESGTADDNDGPTNENGGDGYVN"
     gene            6386..6832
                     /locus_tag="VF240_00245"
     CDS             6386..6832
                     /locus_tag="VF240_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX7173697.1"
                     /translation="MRIKNVVVVASLLLACAPQMPRAQENTNRPRIGPIRILRPDLIV
                     KEYQFAPTNNKGLRVRVANIGNGNAGACVLRLTIRKINGVAVGRTMDMNVPPIAAGAD
                     DWITFIADGILPKAVDIKDTTFKLNVDFHKVVSESKENNNEKWHNL"
     gene            complement(6982..7140)
                     /locus_tag="VF240_00250"
     CDS             complement(6982..7140)
                     /locus_tag="VF240_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173698.1"
                     /translation="MLTAKRQLAGVRTEAERNLYERKCAAVDRQLDELVYELYGLSAG
                     EIALVEAG"
     gene            7374..10562
                     /locus_tag="VF240_00255"
     CDS             7374..10562
                     /locus_tag="VF240_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX7173699.1"
                     /translation="MATTNDARQKRLAQMDWDDIEGALAPAASRRVAGRTRSAARSSA
                     DAAALRDYFGDAEYEYLQKLASHAASMRSRATEPLPNVVLVPGIMGSALSSTRGADID
                     LIWVNFARLALGRVERLKLNEDGTRDADSTRAVSASALVQLAYARTVLWLRARWNVEP
                     YAFDWRKDIDAASDGLAKHIRAKFGGRPAHIVAHSMGGLVSRNFIRRERELWNRMRDA
                     EGERGGRLIMLGTPNYGSYAIPQVLTGTETLIKWLARADVRHSRAEVVEILNTFVGSY
                     QMLPAPSRIPSATQAIYRASTWGDHPVSERHLQRAMEFHDNLEKDNTVDPTRMFYVAG
                     SGRETLSGLRVLSPGEFDYTTTFAGDGRVTHELGLLKDVPTYYADCSHGDLATDEMVL
                     TAVEELLGHGRTASLPQQPPVARSARAASARWTRGAGQQEVAEELKTVARQIDEGTAD
                     PREVRKAERTLARVIMGRDVEPEEIPHVAEEDENARKRQQRIDLKVRVVRGDITRIDT
                     PLVVVGHYQGAAPTGSVGAVNRAIDYWISRAGEHGMIGAGLGQLFFIPTPGRRMKARS
                     VLLAGMGEFGKFRRDSDARFLMMNVTLAAATLGLDRFATVAIGGGRGNMSKEAALRGM
                     VQGVCEALHRLPARRGAASLLRQLVVVENDPQQFNEVLDILEGFAEKPPVEELSLKVS
                     KQIMRPTRAKQKAAARPSDLSEGADETRIHIERSGDSFRFSAITNNAVIPVRDEPVNP
                     KFLQEAAERLMSTLKRQDQEKYGQLLYSYVFPQDFHQVVDTGRDLTLVLDRTTAGLPW
                     EMACYSGPQGMTFLGPHLKLTRQFRTRLSSAPGVTPPPKRRLKALVIADPAREHDLQL
                     PGALREGRAVVEVLNAFRSDKAHQLEIDIVERIGSVECDAVEILALMLNENFDLIHYA
                     GHGVFDAEDPANSGWVFGRKDDGSLLTLSAREIFKARRVPRLVFANACFSAVVRAGQP
                     SSADDMNRDLAGMAEAFFERGVQNYIGAGWPVGDTQAVDFADAFYRRALSGGTLGTAV
                     SEGRLAILGQGSTWGAYHHYGRAGAVLVGPKD"
     gene            10672..10977
                     /locus_tag="VF240_00260"
     CDS             10672..10977
                     /locus_tag="VF240_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7173700.1"
                     /translation="MKFPGGMNIQQMMKQAQAMQEKMQQEMETMVVDATAGGGVVTVQ
                     MRGTKEVESIKIDPEAVKEGDVEMLQDLIVAAVNEAGRKIDEAMKSKLGGMIPPGLL"
     gene            11068..11682
                     /gene="recR"
                     /locus_tag="VF240_00265"
     CDS             11068..11682
                     /gene="recR"
                     /locus_tag="VF240_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7173701.1"
                     /translation="MLEYSEPVTKLIDEFKRLPGVGGKTAQRLAFHVLRMTGAEVERF
                     IAALEEVKLKIISCTVCNNLTDVDPCRFCTSTGRDRSIVCVVEEPYNLVAVEKTRSFN
                     GLYHVLHGSLSPIRGLGPDDLRIQNLLPRLRPENNEGVEVREVILATNPNTEGEATAS
                     YISRLLKPLGVRVTRIAMGMPVGSDLEYVDEVTMDKALTNRHEM"
     gene            complement(11814..12296)
                     /locus_tag="VF240_00270"
     CDS             complement(11814..12296)
                     /locus_tag="VF240_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173702.1"
                     /translation="MRSNGEGFLKKTLMAFAIFLMLPTFAVATQAQQGWGWGQNDDRR
                     RERRERRQQRREVRRERRDDRRDDRGVYRNDGYYGNDNGYYGNNNARQTALNAGYNNG
                     IEEGRKDRQRGERFEYRDESDYQKATEDYSSRLGDRNLYQQYFRQGFANGYRTGYNGY
                     "
     gene            complement(12631..13257)
                     /locus_tag="VF240_00275"
     CDS             complement(12631..13257)
                     /locus_tag="VF240_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7173703.1"
                     /translation="MMPKLIPSQEDVMDILKKTGAYREGHFVYPSGRHTPHYFQMPLA
                     FRYYDTARVLAVALSRKFRIQKDISSTLPKVATISPGPGGIPVAFGVREALSAEQIYW
                     AEREEGSRMFRQYVNKGEVHPCIIVDDISRTGDAINETVELVRQLGAKVIGCGTIVRF
                     KTAPDHIDIPGDDPIPIVSLVEFDSKWYDEGADCSDCQKDAPVEHVRF"
     gene            complement(13387..13869)
                     /locus_tag="VF240_00280"
     CDS             complement(13387..13869)
                     /locus_tag="VF240_00280"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7173704.1"
                     /translation="MWAKLLGAMWRGSPKGVRRWGVWLTQPRFTVTAGAVVEDERGRV
                     LLLHHVFRKGSRWGIPGGFLTKGEQAEDALRRELREEIGMEIESAELALTRTLAEPRQ
                     VEIIFRCRARSAALPKEKLSMEVDGAAWFARDSLPPELGRDQRHLIDTALAARANPRV
                     "
     gene            14088..14957
                     /locus_tag="VF240_00285"
     CDS             14088..14957
                     /locus_tag="VF240_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173705.1"
                     /translation="MSTEANEAKSAAVAAPKPEAGAVAKEAPTEQQKSEPGVPRRFLP
                     GQTVCNEKNEKGKLCNGHLKQMSTQHASQVHLRGDDSLHKCQTCGALYMGPPQGHVRD
                     TRKQERFVERELAAILQAAGGTLPAYDYPTGSQHAPVATRRAVKPAAATATAATNPAT
                     AAAETKPPAPAGETPEQKKARLIAEHKAKLAAKQAGAPPAAATATDKPAPPAETAAPP
                     PSPTAGTDSSSEADSHRPAAEAQAATGQASAAPMSPAAATASPAAGAETVADLAPEAS
                     TELTPGAPKDESK"
     gene            complement(15069..16076)
                     /locus_tag="VF240_00290"
     CDS             complement(15069..16076)
                     /locus_tag="VF240_00290"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173706.1"
                     /translation="MIIESTAPTRVDLAGGTIDIWPLYLFHPGATTVNFAISLYARCR
                     IETRDDDRVILESRDRQVSYDTRLADIEQLAHEERLELIAKLVHFFKPETGFHLIAEG
                     MAPAGAGLGGSSALAIACIGALNRLVGNRYPEEKFIMLAANVETTVIRVPAGFQDYYG
                     AFHGGANAIHFRADGVRREALDVDAAALEDRIVICYTGEPRMSGINNWEVTKSHIDGD
                     PVIFQLFEGIRDAGRAMREALGANDFRRAGEIMRAAYPNRKRLAPNITTPQMEALVEK
                     ALANGAEAAKVCGAGGGGCIAFLCTEGARESVERALAQEEGAEVLKWSFARDGLTVRE
                     S"
     gene            complement(16175..16900)
                     /locus_tag="VF240_00295"
     CDS             complement(16175..16900)
                     /locus_tag="VF240_00295"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7173707.1"
                     /translation="MDLEFFNELVGRYGPWAIFFLTMVEGDITLLLAGVLAHGQVFGD
                     YSFGMVLLAGTLGGVASDNIAYFLGRGARTGVSEYRFYRAARPRLERLMDTFGPLSIF
                     LSKYTYGLRWASCIFYGVVHMPYLRFLLLSFASCFVWVVTLAGVGYFFHSAIYNLIGD
                     FHNLGIGLLVIVAVGVVGFYLVERYWLSKKVEEADPATIQKLEQAAEDKIHEIKEELQ
                     EHLPATLARRKEPPKKRGRTEGD"
     gene            complement(16974..17432)
                     /locus_tag="VF240_00300"
     CDS             complement(16974..17432)
                     /locus_tag="VF240_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7173708.1"
                     /translation="MNLRTILLPTDFSECAAHAVPVAAQFARQAGARLLCLHVVETVV
                     PAVGWSPVAEPLPLADISERLEDSATRELPKLARTAECEGLDVEEVIAHGEPASEIVR
                     VAKERGADLIVISSHGRTGLGRILFGSTAESVVRYAHCPVLVVKPTQGDK"
     gene            17665..18627
                     /locus_tag="VF240_00305"
     CDS             17665..18627
                     /locus_tag="VF240_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7173709.1"
                     /translation="MTTPPAASTQNYVGVEVSGATLRAALVSATGEVLGRREAALERD
                     GVAAQVARAASELRGESEGSGTVAGIGIGLPGLVNPATGNVVISTDLPALARGDLRTE
                     VEKATGLPALLENDANAAAYGEYLAGAGRGSRHMFYITIGLGIGGAFIFDGRIWRGAS
                     GLAGEFGHITIDPEGEECACGNTGCLETVASAPNIVRRTHDRLFRDNTSSLSRLALNR
                     DFAASDIAREATAGDDFARLMIERTGRYIGTGIAAVINLLSVERIVLGGGVMEAGALI
                     LDPIIREAGRRSFQPCFEATQIVAASLGPDAVPVGAALLARAAS"
     gene            18691..19515
                     /locus_tag="VF240_00310"
     CDS             18691..19515
                     /locus_tag="VF240_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173710.1"
                     /translation="MNLTTPRRKLPPAVLLFVLAASALAPVGCQKPPRESGARPQKKA
                     TPDQSALLEQIFQKYTEAVGGEEAIDRVESYALKGAFQMTGSELTLPVEIFVKKPDMT
                     LMVIEVPRVGRIRRGVSAEGGWVQTPFAVVSTEESPGEIVEVERDHDIYQAGRIRRLY
                     REVKLEGQARLKGRDVYVVEGKADKGPAEKMLFDKETGLLLRWDIVRRPPGKPTVFAR
                     VYLDDYRDVGGVRVPFTIRYFFEPREFTLRLNDVRHNVPLDDSMFAKPAARAGGRD"
     gene            19729..21171
                     /locus_tag="VF240_00315"
     CDS             19729..21171
                     /locus_tag="VF240_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX7173711.1"
                     /translation="MKNVAELFRLDYRSLALYRVLLGCVLLYDIAARALEVAAFYTDG
                     GVLPRAPLVEQFQSEWSWSLHLLSGASAVQYVLFALAAVGALALIVGYRTRVATLVSL
                     VLLISLHNRNPLVLSSSDQLMRVLLFWTLFLPVGAAYSFDRARKGAAAAALSRPSNAS
                     VGTAAFILQICLVYWFTAALKSDRAWWHEGSALYYAFNIDYLTTDLGRVMLTFPGALR
                     ALSYLSIWLEAVGPALLFFPRKNGAIRTAVVFAFILFHLCMTPFLTLGTFPFVCAAMW
                     SSLLPAEFWERVGKNFGRLDGWAARSGAPAVARTPAWMNVVAGVFFVYVLFWNFGPVT
                     AQRVGVPARVGWIGQVTGLAQAWRMFSPVPKKDDGWYVIPAKLRGGAEVDLFRNGAPV
                     DWAKPDSVVALYPNSYWQKYTENIWLRDFASARLHYARYLCRTWNAAHGGDEELRTFD
                     IVYMREDTPPPGQPVPVAEPVIIWQHDCFK"
     gene            21294..21494
                     /locus_tag="VF240_00320"
     CDS             21294..21494
                     /locus_tag="VF240_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173712.1"
                     /translation="MIDVERLKIQYVTNEAGEKTAVILSIEEFKELLEDIEDLATAAE
                     RRDEPTVAHDDVVTELKRDGLL"
     gene            21481..21744
                     /locus_tag="VF240_00325"
     CDS             21481..21744
                     /locus_tag="VF240_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7173713.1"
                     /translation="MDSFRVEWKRSAIKELRNLPRNVVARIMRAVEQLPANPFPPGVR
                     KLAGSDHTYRIREGDYRIIYSVVSMTLLIEIVKVGHRKDVYDR"
     gene            21772..23571
                     /gene="lepA"
                     /locus_tag="VF240_00330"
     CDS             21772..23571
                     /gene="lepA"
                     /locus_tag="VF240_00330"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX7173714.1"
                     /translation="MNPERIRNFSIIAHIDHGKSTLADRLLQLTGAVAERDMEAQVLD
                     DMDLERERGITIKSHAVRLDYRARDGHDYVLNLIDTPGHVDFSYEVSRSLAACEGALL
                     IVDASQGVEAQTLANTYLAIANNLELIPVINKIDLPSAEPEKVKEQIEHVIGLDTHNA
                     VLASAKTGLGVPEILEAIVRDIHAPAGDPEAPLKALIFDSWYDSYRGVIVLFRIIDGH
                     VKPGMKIRFFNTGRDYQVETLGVNRPRPTLIDRLGVGEVGFLTASIKTVADVQIGDTI
                     TEAARPTKEAWPGFQEIKPMVFAGLYPVEANQYEELRDALDKLRLNDASLFYEPESSV
                     ALGFGFRCGFLGLLHMEIVQERLEREFDLELITTAPGVRYRVTTTDGEVIEVDSPAKM
                     PDAVRIAKIEEPVIEATILSADDYLGGILPLLEEKRGVQKKFEYIADKRVMLVYELPL
                     NEIVLDFYDRLKSVSRGYASLDYHFSDYWDSDLVKLDLMVAGEPVDALSVIVHRAGSQ
                     AKGRLLAEKMRQLIPRQLFEVPIQAAIGNKVIARETVKALGKNVIAKCYGGDISRKRK
                     LLEKQKEGKKRMKRVGRVEIPQEAFLAVLKVNE"
     gene            23624..25849
                     /locus_tag="VF240_00335"
     CDS             23624..25849
                     /locus_tag="VF240_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7173715.1"
                     /translation="MTTPSPRRAAGFLLRCIALFSLLLAASAPSSQLARAQDPRRDDD
                     DEVLRVSTNLVQTDVTVLDKQGRPVTDLRPEQFELKVDGQTQPLLFFERVTAGSAGEA
                     SALRRSTRGATPTVSTTAASASATRPAPPRGRSVIFFIDDLHLSSEGVARARDILLSY
                     IERRLAPGTPTLIASTSGQIGFLQQFTDDRAVLRAAARRVRYQSTAILDFDQPPMSER
                     IALEIDRGNRGALDYYVVSWIRDNPSRSPTRRDTAEQVVRGRARTIVERAAQVSEATI
                     SSLESAVGARGPEPGRRILFFVSEGFTLTNRKRDIGDKYRRVIAAAARAGTVVYTLDA
                     RGLAVPMLDASSGMRADIELETDLTVVSPSADMAAQQEVLRGLAADTGGRALLNTNGL
                     EQAVESALGETAEYYVLAWRPEAAETAGRSPQFRRVEVSVRGRPDLTVRVRSGFLDAR
                     ADDARQRVASGAAGSATPAVRSSNATLSAVIAEAAERRALPVELYAIFNGETGSPPSL
                     TALVQLTDEPVRFAPAGEKLQAEVTVACVVLDDTGKTVFSEGRTLTLTRAAPGGGASR
                     VITQFPAQLTKPGLYQVRVAAHDARGGLTGSAFQWVEVPDLKAGRLALSTPVIVERHT
                     VTATTAPETPQAVSLNVDRRFARASRLRAELYVYNAARAAGGANVEMEIKVWRDGRVA
                     LSAPPHKLADAGAADPSRIRYDAEVPLRGLAPGPYILEVSAADRTTRAAATRQIAFTV
                     E"
     gene            25920..27569
                     /locus_tag="VF240_00340"
     CDS             25920..27569
                     /locus_tag="VF240_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173716.1"
                     /translation="MKFPLVALALLLSADLASAQHHAPASKEQPPPVRLIAELGAYRF
                     PIARTPHAEAQKFFDQGMILVYGFNHDEAARAFRRAAELDPAMSMAHWGLALALGPNY
                     NEATISPERLRAAHDAVRKGLALAAKGPEHERAYLEALAKRFSSDANADQKKLWVEYK
                     NAMADMTRRYPDDLDAATLYADAAMITNAWKLWSPEGKPAEGTEEIVAVLESVIHRDP
                     EHIGAHHLYIHAVEASPHPEWALASADRLAVLSPAAGHLVHMPAHTYMRVGDYERAAL
                     ANEFAAKVDQEYLDKGGLRGIYTAAYYSHNLHFLAAARSMQGRYAESVAAARRLEQNV
                     RPYLKEMPFFESFLPTRSLIAARFGRWDDVLKMESPEASAPITKALWHWARGMAHAGT
                     GKLSDADIELKTFTAAAQAIPAEAGYGQNTARSVLKIAEHFLNSRIAAARGDRKSAIE
                     FLRQAAAAEDALLYDEPPGWYHPLSRESLGGALLLDGQHAEAERVFREDLRRNRRNGR
                     SLFGLAESLKAQGRAHESELVRREFERAWKGADTKLRVEDL"
     gene            27690..>27871
                     /locus_tag="VF240_00345"
     CDS             27690..>27871
                     /locus_tag="VF240_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173717.1"
                     /translation="MKRWLLLLLLSAAALALVAVPVWLIQPFSPQTPRALEVGYALRR
                     WSPLVTLALAAGGLAL"
BASE COUNT         4825 a         9342 c         9074 g         4630 t
ORIGIN      
        1 ccgcagtggt gcgcgctcgc gaggtcggca gggacaactg gaagaaggag gcgggctacc
       61 accgccgctc gctggccgag acggcgatga tgcgcctgaa gacgatcttc tccgacaggc
      121 ttcgggcgcg agagtggcgc aggcaagtta ccgagttgag agtgaggtgt gttgcgatga
      181 accgaatgac gagcctgggg atgccgcaga gctacgccgt ctgagtcatc ggcgaagcgg
      241 gtgaagtcta ctcaccctca tttatgcaac aacgcctagt agacgtacta ttagccgcca
      301 gtaaagacgc ccattcgggc gtctaatccg gggccgctca cggtttcccc ttgattttct
      361 caaccccgcg acgtattttg ccgcccaagc ctcgcccgat tagacgccct cacccgtcag
      421 gagcacctca ccatgtccca actgctcgac agggtgcgcg gagccgtccg cgcccgccac
      481 tacagcatcc gcaccgagga agcatacctc cgctggattc gtgagtacat cctcttcttt
      541 aacaagcgcc acccgtcgga actcggcgcc ccggaggtcg gcgccgtcgt ctctcacctg
      601 gccgtgcagc gcaacgtcgc cgcctccacg cagaagcagg cgctctcggc gctcctgttc
      661 ctctaccgcg aagtgctcga ccagcccatc gagtgggtaa tatccggcta ctaaactcag
      721 cttgaactta gctcaaaatg gaagaaggcc cgcttctaac tttatgttag tgcgggcctt
      781 ccggtgaact gatgggacta gccttcccga ccgctcacat caatcctttt gagtgggtcg
      841 ccgcttcgaa cggagtcgtg aacgaactct cgatagcgcc gtccgaagct tatcaggctt
      901 cagccacctc actgtgataa gaactcatac ttgtttgatc acctcctttc cagtgttacc
      961 gccgaatata gacgacaaca aattgtgcgt caaccgttat tttcaacagc catacggacg
     1021 gccatctttc aagccccgcg tcgatagacg agccttccgg cgatgaacac ggcccatgcg
     1081 tgctcgacgc cgaagaagta ggccagctcg cggtagtcgt gggcgtcgtg gacgacgaag
     1141 tcggccagtt tcccttcttc gagcgtgccg aggtcgcgtc cgcgccggag gctgtgggcg
     1201 gcgttgacgg tcgccgccga aaggccctcg gcgggcgtca tcttcatgtg cgtcgaggcg
     1261 agcgagagca ccatggtcat cgagggcgtg ggcgacgatc ccggattgaa gtccgtggcg
     1321 acgacgacac tgaggccgcg ctcgatcatc tggcgcgccg gcgggtactg ccgcgagccg
     1381 agcgcgtaga ccgagccggg caaaagcacc ggctgcacgc cggccgcgcg caaggcttcg
     1441 atcccggcgg cgtcggcgtg ttcgaggtgg tcggccgtcg cggcgccgag ttcggcggcg
     1501 aggcgcgcgc cgcccgagag tgagagctgg tcggcgtgga cgcgcagtcc cagaccgtgc
     1561 gcgcgcgcgg cgagcagaat ctcccgcgac tcatcgacgc cgaagacgcg cccctcgcag
     1621 aacacgtcgc agaactccgc cagcccttcg ccggccacgc gcggcagcat ctcttcgatg
     1681 acgagtcgga catactcggc gcggtcggcg cggtgttctt cggggacttc gtgcgcgccg
     1741 aggaaggtcg gcacgaaacg cagcggcgtc tcctcgccga gacgcctgac gacacgcagc
     1801 atcttcaact cgtcctcgac cgtgaggccg tagccggact tcgcctcgac ggtcgtcgtg
     1861 cccgtccgca aaaaccactg cgcccgtcgt ttcgactccg cgaagagttc gtcctcggag
     1921 gcggcgcgtg tccgcctgac ggtcgcacgg atgccgccgc ccgccgccgc gatctcctga
     1981 taggtggcgc cctcggcgcg tctctcgaac tcgtcggcgc gattgcccgc gaagaccggg
     2041 tgcgcgtgcg cgtccacgaa gccgggcgcg acgacccggc cgcccgcgtc gaacacctcc
     2101 gcgtccctct ctatcagcgg ctcgatctcg gcgcgcgtcc ccacacgggc gactcgttcg
     2161 ccactgacga gcatcgcgcc gtcctcgatg atcgacaact cgcgcagctc cgcgccgaca
     2221 cgcggacggg ccgggccgcg cagggtgacg agctgcgagc agttgacgac aacaaaagtc
     2281 tcagacactt gcggcacgct ccttcctccg cgcgcgccgc gagagcaggg cgtagacgag
     2341 gagtgtgagg agcaggaggt cgatgagtcc cgcggaggcg accgccgcga agctcggccc
     2401 ctcgtcggcg tcgcccgcag cgcgcgggaa gacgaggcgg gtcagcggga gcggccacgc
     2461 gaggaggtaa tagaagaagc ccgacgccca cgcgggcggg tggcccggcc tgtaccattt
     2521 gttcaactgc gccagcgcca ccgtgtcggc gaacatcacg acggccgcca gcaccgccga
     2581 cagcagccaa cgcttccatg tcttcttaat catcgcgctc tctctcgaaa acagtaccgc
     2641 gagcggtagc gagcgggtgt ccgtgccgag gacacacccg ctcgctaccg ctcgcggtac
     2701 tgtgcttgag tcaatgaaat ccctttaggc cgacgaagtg tggtcgtgga cgatgcgcca
     2761 gccggcggcg gtgcggcgga agaggagcgt gaagcgtccg cgcgggtcgc cggagacgcc
     2821 cgtgagatgc cagcggccga cggccgcgac gtagaagggg ctgaccgggc ggaactccag
     2881 ctccgtgaat tcgagcgtgc ccatcttctc gcgggtgtcg taggttcgct tgtagcggtc
     2941 gaggacggtc tgccagccgc gcgtgacggc gtcgccggag acgaaggtgg tcgtctcctc
     3001 gcgcgcgtag ccgtccatga agccctcgat gtcgccgcgg ttccacgccg cctgctgctc
     3061 ctcgatcacc ttgcgcacgg ccgtcaccgc ctgctcgccc tccttgtccg agacctcgga
     3121 gacgttggcg caagcagagt agagcgcggc ggccgaggcg gcgagcgacg ccgcgaggcc
     3181 gagagaaagt ctgcgtttca tgtcagcgta cacctttcga cgagatgatt tgcagtcccc
     3241 gcctgagtgt tctttgtcgg tggcggcgcg ccgaatcata cgcccgcgcg cgcggccaac
     3301 caagcgcgca catccaagcg catggcggag gtgaactcgt ggccggcgtc gtagcttttg
     3361 gtctccacgt cggcggcgcg tgcgcgcagg cgcgcggcgt agtcggcgac gcgctcgggc
     3421 gggtagaact cgtcgcgcgc gccgtggagg taaaggacgg aggcgcgcgt tcgccggtag
     3481 agcgtgctct cttcccagtc gccgggcaga ccgccgcaga cgccgatgac gccgcgcaag
     3541 cgttcggcgt gcgtgaaggc gaagcggaag ttgagggcgc aggtctgtga gaagccgagc
     3601 aggaaaaccg cgtcggggcg aaccactgac tggcggtcga gcgcatcgac caagtcgagc
     3661 agcgcgcggt ggtggagcgc gaccgactcc tcggggcgga agttggtgag ccagccgaag
     3721 ccgtagcgca gcgggcctcc ggacacgcgc gcctccttga tgtgctggtg cgggccttgc
     3781 agcgacgcga cggcgaaccc ctccggcgcg acctctcgcg ccacgcgcat catccagccc
     3841 ttgttgtcgc cgtagccgtg cagcgcgacc aagagcggcg cgggagtctg cgccggcgcg
     3901 tggagatcgt agtagagctt gatctcggcc gtgagcgtgc gctcagtttg caaaggttct
     3961 cgctcggtct ggttcatcat gtggaaactt tcaggctcag gccacggctg gcaatcgagc
     4021 acatggagcc gggtcagtgg acttttagtt gacgtatcca tcgccgccgt tttcgttcgt
     4081 tggcccgtcg ttatcatcag ccgtcccgct ctcctgcgtc gcggggccga gtgacagcgc
     4141 gttgtcttct tcgaggctcc agacttcaaa gaggtctggg ggtacttcgg tgacgaagcg
     4201 ggaggggcgc tggaggaggg tctggcgggt gtggtcggtg gcggtgaggg ggtaggcgat
     4261 gtagagctgg tcgcgggcgc gggtgatggc gacgtaccag aggcggcgct cctcttcttc
     4321 gccctcggcg tcgcgcaaag agcgggggct ggggaagcgt ccgtcggagg cccagatgag
     4381 gaagacgacg cgccattcga gacctttggc ctgatggacg gaggagagcg tgagcagttc
     4441 gtcttcttcg ccgccggaga ctacgtcctc gccggttaag ggttgggggc cgccgtaacg
     4501 ttcggtggag aggagggcga gttcgccgag gaagtcttcc gtggagttgt agcgggcggc
     4561 gtagagggcg agctggcgga ggtcttcgag gcgcgtctcg gcgttctcgt agttggcggc
     4621 gaggtaggat tcgtagccgc gcgcgaggat gagttcgatc tggcgcgccg gggtggcgcg
     4681 ggtgtcgtcg gcgagcaggc tttcgacgag ggcgcggaac tccgtccagc ccgtgccgcg
     4741 cgtgcggacg gcttcgtcgg cgccgccgcg cacgactaaa gcgagcgggt cgggcgcgta
     4801 ggcgaggcgt tcccagacgc gcgcggccgt cgcctgtccg acgttcggga cgagacggag
     4861 cacgcgcttc cacgcaagct cgtcgcgcgg gttgacgacg aggcgaaggt aggagaccac
     4921 gtccttgatg tgcgcctgct cgaagaagcg gacgccgctg cggacggcgt aggggatgcc
     4981 gcggcgcgtc agctcaagct ggagttcgag tgagtgatag tgtgagcggt agaggatggc
     5041 gatctcttcg agggggacgc cctcgtcgcg cagctcaagg acgcgggagg cgacgaaggc
     5101 ggcctgctgg tcggcgtcgc ggcaggggac gagcgcgggc ggcaggccca aactggggcg
     5161 cgcggcgcgt agagtttttt caaactgctt gcggttggcg gcaatcgaga cgttggcgac
     5221 ggcgaggatt tcgggcgtcg agcgatagtt cgtctcaagg cggaaggtgc gcgcctcggg
     5281 gtagcgtttc gggaactcgt agatgttcgc ccactccgcg ccgcgccacg cgtagatgct
     5341 ctgcgcgtcg tcgccgacga ccatcacgcc ccggtgcttg acgccgagca ggtcaacgat
     5401 ctcggcctgc aacttgttcg tgtcctgata ctcgtcaacg aggatgtgct cgaactgctc
     5461 ctgatagaga cgcgcgactt cgtccttctc ttcgagcagg cgcttccagt tgagcagcag
     5521 gtcgtcgtag tccatcacgt tgcgctcgcg cttgcgctcc atgtagacgc ggtcaacttg
     5581 tttgacggcc gccgtcagcg gctcgaagta agggtgccgg cgcgcgattg tatcttcgat
     5641 gggctggtcg gtgttggtgg cgaagctgat cacgtcctgc aagacctcgg ccttggggaa
     5701 gcggcgcgcg cgcgtgtcca cgcccgcctc gacgatgcac gcggagagaa gatcgcgcgc
     5761 gtcctccgag tcgagaatcg tgtagttcgc ggtgtagccg attgattcgg cgtggcggcg
     5821 caagcacagg ttggcgatgc ggtggaaggt gccgccccag acgcgccgcg cggcgtcggc
     5881 ttgcgcgccc gccaactgtt cgacgcggcg caacatctcg cgcgccgcgc ggttcgtaaa
     5941 cgtcgcgagc atgatgcgcg ccggcgagac gccctgctcg acgaggtagg cgacgcggta
     6001 ggtgatggcg cgtgtcttcc cactgcctgc gcccgcgatg acgagcgccg cgccgggcgg
     6061 ggccgtcgcc accgccaact gttcggcgtt caactcctcg cggtagcgcg ccagcctgtc
     6121 cggcagcccg gcccccgcgg gccgcttgat gacgtatcgc ttcatcgttc gtggcttata
     6181 gcacgccgtc gggccgcgcg cgaagcggat tgttgtcggc cgcttcggac gagcgttgac
     6241 ttgactccga tgttgacata tcgtcgctgc cgcttctgaa acgctcgaga gatggaaacc
     6301 cacacgcgtc gcccgccccg gcgtgcgtca acccgaaccg gcgtgtcttc acgacagccg
     6361 ccttcagccg aaacggagga acccgatgag gattaagaac gtcgtcgttg tagccagcct
     6421 gctgctcgcc tgtgcgccgc aaatgccgcg cgcgcaggag aacaccaaca gaccgcggat
     6481 cggcccgatc aggatactca ggcccgacct gatcgtgaag gagtaccagt tcgccccgac
     6541 caacaacaaa ggcttgcgcg tgagggtcgc caacatcggc aacggcaacg cgggcgcgtg
     6601 cgtcctgcgt ctgaccatcc gcaagatcaa cggcgtcgcg gtcggccgga cgatggacat
     6661 gaacgtcccc cccatcgcgg cgggcgccga cgactggatc accttcatcg ccgacggcat
     6721 tctgccgaaa gccgtggaca tcaaggacac gaccttcaag ctcaacgtag acttccacaa
     6781 ggtggtgagc gagtcgaaag agaacaacaa cgagaagtgg cacaacctct gagcggcgac
     6841 gcgccggagc atcacacacg cgttagccgg tagcccgacc gcgggggagg gccgaacgcg
     6901 ggaaggcgga agtcgaaagg ggaaagtgaa gcagcggcaa acgctctttc actcttccct
     6961 ttcgacttcc gccttcttct ttcagcccgc ctcgacgagc gcgatctccc ccgcgctcag
     7021 cccgtacaac tcgtaaacca gttcatccaa ctgccggtca accgccgcgc acttgcgctc
     7081 gtacagattc cgctccgcct ccgtccgcac tccagccaat tgtcgtttcg ccgtcaacat
     7141 ttgctcgact aacttaacaa cctccttcgg ccgctttggc gatgcggaat gaggattgac
     7201 gcgccttcgg ttttcacata tcgtggcgcg gtcttgctaa tgcttcacgg cgtttccgac
     7261 cgcgcgcgcg gcgcggtttc tcaggccgct tttccttttc tcacgctcct gacagcggcg
     7321 ccccttgaac gcgacgctcg ccgctcgcgc ggcgggcgag gaggccacac atcatggcga
     7381 cgaccaacga cgcacggcag aagagactcg cgcagatgga ctgggacgac atcgagggcg
     7441 cgctcgcgcc cgccgcgtcg cgccgcgtcg cgggacgcac gcgcagcgcc gcgcgctcca
     7501 gtgccgacgc cgcggctttg cgcgactact tcggcgatgc cgagtatgag tatttacaga
     7561 agctcgccag ccacgccgcc tcgatgcgct cgcgcgccac cgagccgctc cccaatgtcg
     7621 tcctcgtccc cggcatcatg gggtcggcgc tctcctcgac cagaggcgcc gacatcgatc
     7681 tcatctgggt caacttcgcg cgcctcgctc tgggccgcgt cgagcggctc aagctcaacg
     7741 aggacggcac gcgcgacgcc gactccacgc gcgccgtctc ggcaagcgcc ctcgtccagc
     7801 tcgcctacgc gcgcaccgtc ctgtggctgc gcgcccgctg gaacgtcgag ccttacgcct
     7861 tcgactggcg caaggacatc gacgccgcct ccgacggttt agccaaacac atccgcgcga
     7921 agttcggcgg ccgtcccgcg cacatcgtcg cccactcgat gggcggtctc gtctcgcgca
     7981 acttcatccg gcgcgagcgc gagctttgga accggatgcg cgacgccgag ggcgagcgcg
     8041 gcggacgcct catcatgctc ggcacgccca actacggctc ctacgccatc ccgcaggtgc
     8101 tcaccgggac ggagacttta atcaagtggc tcgcccgcgc cgacgtgaga cacagccgcg
     8161 ccgaagtcgt cgaaatcctc aacaccttcg tcggcagcta ccagatgctc cccgcgccga
     8221 gccgcatccc ttccgccacg caggccatct accgcgcgag cacgtggggc gaccaccccg
     8281 tctcggagag acacctgcaa cgcgccatgg agtttcacga caaccttgag aaagacaaca
     8341 cggtcgatcc gacgcggatg ttctacgtcg cgggcagcgg gcgcgaaacg ctctcgggcc
     8401 tgcgcgtcct gtcgccgggc gagttcgact acacgaccac tttcgccggc gacggccgcg
     8461 tcacgcacga actcggcctg ctcaaagatg tgccgaccta ctacgccgac tgctcgcacg
     8521 gcgacctcgc caccgacgag atggtgctca cggccgtcga ggaattgctc ggacacgggc
     8581 gcaccgcttc cctgccgcag cagccgcccg tcgcgcgctc ggcgcgggcc gcgagcgcgc
     8641 gctggacgcg cggcgcgggc cagcaggagg tcgccgagga attgaagacg gtcgcgcgcc
     8701 agatcgacga ggggacggcc gacccgcgcg aggtgcgtaa ggccgagcgc acgctcgcgc
     8761 gcgtcatcat gggacgcgac gtggagccgg aagagattcc tcacgtcgcc gaagaagacg
     8821 agaacgcgcg caagcggcag cagcgcatcg acctcaaagt ccgcgtcgtg cgcggcgaca
     8881 tcacgcgcat cgacacgccg ctcgtcgtcg tcggccacta tcagggcgcc gccccgacgg
     8941 gctcggtcgg ggccgttaac cgcgccatcg actactggat cagccgcgcc ggcgagcacg
     9001 gcatgatcgg cgcggggctg ggacagctct tcttcatccc cacgcccggc cggcgcatga
     9061 aggcccgctc cgtgctgctc gccggcatgg gcgagttcgg gaagttccgc cgcgacagcg
     9121 acgcgcgctt cctgatgatg aacgtcacgc tcgccgccgc cacgctcggc ctcgaccgct
     9181 tcgccaccgt cgccatcggc gggggccggg gcaacatgtc gaaggaggcg gcgctgcgcg
     9241 gcatggtgca gggcgtctgc gaggcgctgc accgattgcc cgcgcgacgc ggcgcggcct
     9301 ccctgcttcg acaactcgtc gtcgtcgaga acgacccgca acagttcaat gaagttctcg
     9361 acatcctcga aggctttgcg gagaagccgc ccgtcgagga attgagtttg aaggtgtcaa
     9421 agcagatcat gcgtcccacg cgcgctaagc agaaggcggc cgcccgcccc tcagacttga
     9481 gtgagggcgc ggatgagacg cgcatccaca tcgagcgcag cggcgactct ttccgcttct
     9541 ccgccatcac caacaacgcc gtcatccccg tgcgcgacga gccggtcaat ccgaagtttt
     9601 tgcaggaggc ggccgagcgc ctgatgtcca cgctcaaacg gcaggatcag gagaagtatg
     9661 ggcagctcct ctacagctac gtcttcccgc aggactttca tcaggtggtg gacacgggca
     9721 gagacctcac gctcgtgctc gaccgcacga ccgcgggcct gccgtgggag atggcctgct
     9781 acagcggccc gcaggggatg accttcctcg ggcctcatct gaaactgacg cgccagttcc
     9841 gcacgcggct gtcgagcgcg cccggcgtca cgccgccgcc caagcggcga ctcaaggcgc
     9901 tcgtcatcgc cgaccccgcg cgcgagcacg acctgcaact gcccggcgcg ctcagggagg
     9961 gccgcgccgt cgtcgaagtc ctgaatgcat tcaggagcga caaagctcat cagcttgaga
    10021 tcgacatcgt cgagcgcatc ggctcggtgg agtgcgacgc ggtcgaaatc ctcgcgctga
    10081 tgctcaacga gaacttcgac ctcatccact acgccggcca cggcgtcttc gacgcggaag
    10141 acccggccaa cagcggttgg gtcttcgggc gcaaagacga tggaagcctg ctcacgctct
    10201 cggcccgcga aatcttcaag gcgcggcgcg tcccgcgtct cgttttcgcc aacgcctgct
    10261 tctcggccgt cgtgcgcgcc ggccagccct cctcagccga cgacatgaac cgcgacctcg
    10321 ccggcatggc cgaagccttc ttcgagcgcg gcgtacagaa ctacatcggc gcgggctggc
    10381 cggtcggcga cacgcaggcc gttgatttcg ccgacgcctt ctaccgccgc gccctctcgg
    10441 gaggcacgct cggcacggcc gtctccgaag gacgcctcgc catcctcgga caaggctcga
    10501 cgtggggagc ctatcatcac tacggccgcg cgggcgcggt gctcgtcggc ccgaaagatt
    10561 gagcgacgac ccggcccacg cccgtcgggc ttgcggcccc gttgacgccg ccacgcatgg
    10621 aagataggat ggttggcagg caccaatcac gaagacgaga gggcacgaga gatgaaattt
    10681 ccgggcggga tgaacattca gcagatgatg aagcaggcgc aggccatgca ggagaagatg
    10741 cagcaggaga tggagacgat ggtcgttgac gccacggccg gcggcggcgt cgtcaccgtc
    10801 cagatgcggg gcaccaagga agtcgagtcg atcaagatcg accccgaggc cgtcaaggaa
    10861 ggcgatgtcg agatgctcca ggacctcatc gtcgccgccg tcaacgaggc cggccgcaag
    10921 atcgacgaag ccatgaagtc gaagctcggc ggcatgatcc caccaggact gctataaagc
    10981 cgtgacaagt gatgagtgat aagtgataag agaaagcatc cgccttttct tatcacttat
    11041 cactcatcac ttatcacctc tactcctatg cttgagtatt cagagccagt cacgaagctg
    11101 atcgacgagt tcaagcgcct gccgggcgtg gggggcaaga cggcgcaacg cctcgccttc
    11161 cacgtgctgc ggatgaccgg ggccgaggtc gagcgcttca tcgcggcgct cgaagaggtg
    11221 aagctcaaga tcatttcctg cacggtctgc aacaacctga cggacgttga cccgtgccgc
    11281 ttctgcacct cgacggggcg cgaccgctcc atcgtctgcg tcgtcgaaga accttacaac
    11341 ctcgtcgccg tcgagaagac ccgcagcttc aacggcctct accacgtcct gcacggctcg
    11401 ctctcgccca tccggggcct cggccccgac gacctgcgta tccagaacct gctgccgcgt
    11461 ctccggccag agaacaacga gggcgtcgag gtgcgcgagg tcatcctcgc caccaacccc
    11521 aacaccgagg gcgaggccac ggcgagctac atcagccggc tgctcaagcc gctgggcgtc
    11581 cgcgtcacgc gcatcgccat gggcatgccc gtcggcagcg acctcgaata cgtcgatgag
    11641 gtgacgatgg acaaggccct caccaatcgc cacgagatgt gagggtagct gctagctatt
    11701 agctgttagc tggagaaaaa caaaagcgac ggccggctct cgtcaattac aagagccggc
    11761 cgtcgctttt ttgtctttag ctgaaagcta atagctaata gctaaccgct ttcttagtat
    11821 ccgttgtagc cggtgcggta gccgttggcg aagccctgtc ggaagtactg ctggtacagg
    11881 ttgcggtcgc cgaggcgcga gctgtagtcc tcagtcgcct tctggtagtc cgactcgtcg
    11941 cgatactcga aacgctcgcc gcgctggcga tccttgcggc cttcctcgat gccgttgttg
    12001 tagccggcat tcagcgccgt ctggcgggcg ttgttgttgc cgtagtagcc gttgtcgttg
    12061 ccgtagtagc cgtcgttgcg gtacacaccg cggtcatcgc ggcggtcgtc gcgacgctcg
    12121 cggcgaactt cgcgacgctg ctggcggcgc tcacgacgct cgcgcctccg gtcgtcgttc
    12181 tggccccagc cccagccctg ctgggcctga gtggcgacgg cgaacgtcgg cagcatcaga
    12241 aagatggcga acgccatcag cgtcttcttc aagaatcctt ctccgttgct tctcatcgtt
    12301 tcattctccc tctatttgat tgcccaccga tgtttttttt tggggagcga tccattacgc
    12361 cgttattgtt aacaagcacc gtgccacagg cgaccgacac gtaactgccg ttaaaatagc
    12421 cactttggga gagataaggg cgggcgtggc acacggttag gtgtctcgac gcaccgcatc
    12481 gtgtgcgtta agaatacggg caacgcctca gatttgaacg cggatttttt cgtgagacac
    12541 gatccgcggc ggcgacgcga aacgtcgccg ccacggatca tcgtggagtt gggattagtg
    12601 acgcacggcg aggggcgcgg tcgcctgccg tcagaatcgc acgtgctcga ccggcgcgtc
    12661 cttctggcag tcggagcagt ccgccccctc gtcgtaccac ttggagtcga actcgacgag
    12721 tgagacgatg gggatggggt cgtcgccggg aatgtcgata tggtcgggcg cggtcttgaa
    12781 gcggacgatg gtgccgcagc cgatgacctt cgcgccgagt tgtcggacga gttcgaccgt
    12841 ctcgttgatg gcgtcgccgg tgcgcgagat gtcatcgacg atgatgcagg ggtgaacctc
    12901 gcccttgttg acgtactgcc ggaacatgcg gctgccctct tcgcgctcgg cccagtaaat
    12961 ctgctcggcg ctcagagcct cgcgcacgcc gaaggcgacg gggatgccgc cggggccggg
    13021 gctgatggtg gccaccttcg gcagcgtgct ggagatgtcc ttctgaatgc gaaatttccg
    13081 cgagagcgcg acggcgagca cgcgcgccgt gtcgtagtag cggaaggcga gcggcatctg
    13141 gaagtagtgc ggcgtgtggc ggcccgacgg gtagacgaag tggccttcgc ggtaggcccc
    13201 cgtcttcttg aggatgtcca tcacgtcttc ctgcgacggg atcagcttcg gcatcatggt
    13261 cttcggttct cctgaaaagt tagtggttag aggtcagagg ttagaggtca gtaaaaacca
    13321 cttcattctt ttactgacct ctaacctcta acctctgacc tctgattttt ggccgccagc
    13381 ataccactaa acgcgtgggt ttgcgcgagc agcgagggcg gtgtcgatga ggtggcgctg
    13441 gtcgcggccg agttcgggag ggagcgagtc gcgcgcgaac cacgcggcgc cgtccacctc
    13501 catgctcaat ttttctttgg ggagcgcggc cgagcgggcg cggcagcgga agatgatctc
    13561 gacctggcgt ggctccgcga gcgtgcgcgt cagggccagt tccgccgact cgatctccat
    13621 cccgatttcc tcgcgcagct cccggcgcag ggcgtcctcg gcctgctcgc ccttcgtcag
    13681 gaacccgccg gggatgcccc accgactccc cttgcggaag acgtgatgaa gcaggagcac
    13741 gcgcccgcgc tcgtcctcga cgacggcccc ggccgtcacc gtgaagcgcg gctgcgtcag
    13801 ccacaccccc caccgccgca cgcccttcgg cgagccgcgc cacatcgctc ccagaagctt
    13861 cgcccacatg cgcggcaaag atagcacaga gagcagaggt cagagggcag agggcagagg
    13921 gcagtgaaca agcaatccgg ttttcactga cctctaacct ctaacctctg acctctgctt
    13981 cctgttgacg ctccgcgcgc gttcttcgta agatgcaggc gcgttgtcgg agggagttta
    14041 agagcctgtc tttcttacat cagtgcgaac ggagctagag cgagccgatg tcaactgagg
    14101 cgaacgaagc gaagagtgcg gccgtcgccg cgccgaagcc ggaggcgggc gccgtggcga
    14161 aggaggcccc gaccgagcag cagaagtcgg agccgggcgt gccccgtcgg ttcctgccgg
    14221 ggcagaccgt gtgcaacgag aagaatgaga agggcaagct ctgcaacggc catctcaagc
    14281 agatgagcac gcagcacgcc tcgcaggtgc atctgcgcgg cgacgactcg ttgcacaagt
    14341 gtcagacctg cggcgcgctc tacatggggc cgccacaggg ccacgtccgc gacacgcgca
    14401 agcaggagcg attcgtcgag cgcgaactcg ccgccatcct tcaggccgcc ggcggcacgc
    14461 tccccgcata cgactacccg acgggcagcc agcacgcacc cgtcgccacg cgccgcgccg
    14521 tcaagccggc ggcggcgacg gccacagccg cgacgaatcc cgccacagcg gcggcggaaa
    14581 cgaagccgcc cgcgcccgcc ggcgagacgc ccgaacagaa gaaggcccgc ctcatcgccg
    14641 agcacaaggc gaagctcgcc gcgaagcagg ccggcgcgcc gccagccgcc gcgaccgcga
    14701 cggacaaacc cgcgccgccg gcagagacag ccgcgccgcc gccctcgccc accgccggca
    14761 cggactcatc gtctgaggcc gacagtcacc gccccgccgc cgaggcgcag gccgcgacgg
    14821 gtcaggcgtc ggccgcgccc atgagtccgg cggccgccac cgcctcaccc gccgccggcg
    14881 ccgaaacggt cgccgacctc gcgcccgaag cctcgaccga actcaccccc ggcgcgccga
    14941 aggacgaaag caagtagtga cggacgcgcc tgcgtccgtc taacttccag aacaactcac
    15001 ttctaaaaac gaaagcgcgt ggaaccccgt cgagttccac gcgctttgac gtttcaacac
    15061 aggccctctc agctttcgcg caccgtcagc ccgtcacgcg cgaagctcca cttcaaaact
    15121 tccgcgcctt cttcctgcgc cagcgcgcgc tcgaccgact cgcgcgcgcc ctcggtacag
    15181 aggaaggcga tgcagccgcc gccgcccgcg ccgcacacct tcgccgcctc cgctccgttc
    15241 gccagagctt tctcgacgag cgcctccatc tgcggcgtgg tgatgttcgg ggcgaggcgc
    15301 ttgcggttgg ggtaggcggc gcgcatgatc tcgcccgccc gccggaagtc gttcgcgccc
    15361 aaagcctcgc gcatcgcgcg gccggcgtcg cggatgccct cgaagagttg gaagatgacg
    15421 gggtcgccgt cgatgtggct cttggtcacc tcccagttgt tgatccccga catccgcggc
    15481 tcgcccgtgt agcagatgac gatgcggtct tcgagcgccg cggcgtccac gtcgagcgcc
    15541 tcgcggcgga cgccgtcggc gcggaagtgg atggcgttgg cgccgccgtg gaaagcgccg
    15601 tagtagtcct gaaagccggc ggggacgcgg atcaccgtgg tctccacgtt ggcggcgagc
    15661 atgatgaact tctcctcggg atagcgattc ccgacgaggc ggtttaaggc cccgatgcag
    15721 gcgatggcga gcgcgctcga cccgccgagg cccgcgccgg ccggcgccat gccttcggcg
    15781 atgaggtgga acccggtctc gggcttgaag aagtggacga gcttggcgat cagctccaga
    15841 cgctcttcgt gggcgagttg ctcgatgtcg gcgaggcgcg tgtcgtagga gacctgtcgg
    15901 tcgcgcgact cgaggatgac gcggtcgtcg tcgcgcgtct cgatgcggca acgcgcgtag
    15961 agggagatgg cgaagttgac cgtggtcgcg ccgggatgga acaggtagag cggccagatg
    16021 tcgatggtgc ccccggccag atcgacgcgc gtcggtgcgg tggattcgat aatcataacg
    16081 agagtgataa gtgatgagtg gtgagtgata agaaagagat aagaggcgcc tgccttttct
    16141 tatcactcat cactcatcac tcatcactat tctttcagtc cccttccgtc ctgccgcgct
    16201 ttttgggcgg ctccttgcgg cgcgcgagcg tggcggggag gtgttcttgt agctcctctt
    16261 tgatttcgtg aatcttgtct tcggccgcct gttcgagctt ctgaatcgtg gccgggtcgg
    16321 cctcctcgac cttcttcgac agccagtagc gttcgacgag gtagaacccg acgacgccga
    16381 cggcgacgat caccagcaga ccgatgccga ggttgtggaa gtcgccgatt aggttgtaga
    16441 tggcgctgtg gaagaagtag ccgacgccgg ccaaagttac cacccacacg aagcaactcg
    16501 caaacgagag caggaggaag cgcaagtagg gcatgtggac gacgccgtag aagatgcacg
    16561 acgcccacct cagcccgtag gtgtactttg agaggaagat ggagagcggg ccgaaggtgt
    16621 ccatgaggcg ttcgagtcgg ggccgcgccg cgcggtagaa gcggtactcg ctcacccccg
    16681 tgcgcgcccc gcgcccgagg aagtaggcga tgttgtcgct ggcgacgccg ccgagcgtgc
    16741 cggcgaggag caccatcccg aagctgtagt cgccgaacac ctgcccgtgc gccagcaccc
    16801 cggccaaaag cagcgtgatg tcgccctcga ccatcgtcag aaagaagatg gcccacggcc
    16861 cgtagcgccc caccagctcg ttgaagaact ccagatccat ttgaaagcgg gtgataagtg
    16921 atgagtgata agtgatgaga aaatcctgtt tcccacttat cacttatcac ttatcactta
    16981 tcaccttgag tgggtttgac gacgagcacg gggcagtggg cgtagcggac gaccgactcg
    17041 gcggtcgagc cgaagaggat gcgccccagc cccgtgcgcc cgtgtgacga gatgacgatc
    17101 aggtcggcgc cgcgctcctt cgccaccctg acgatctccg aggccggctc gccatgggcg
    17161 atcacctcct caacatcgag gccctcgcac tcggcggtgc gcgccagttt cggcagctcg
    17221 cgcgtggctg agtcctccag tcgctcgctg atgtccgcga gcggcagcgg ctcggccacg
    17281 ggcgaccacc ccacggccgg caccaccgtc tcgacgacgt gcaggcacag cagacgcgcc
    17341 cccgcctgac gcgcgaactg cgccgccacg ggcacggcgt gggccgcgca ttcgctgaag
    17401 tcagtgggca gcagaatggt acgaaggttc ataacggcca gagaggttag aggttagagg
    17461 ttagaggtca gtagaaaggc aatccatctg ttactgacct ctaacctctg acctctgacc
    17521 tctgcccttc ggcgcgtaat tctacacgca agaggagttg tttgacagtc ccgcgccagc
    17581 gtgtgctacc atccacacca aacggattcg cccgagcggt cgcgaggttc cagcgaagcg
    17641 acgaagcgaa aggcccctga cgctatgact acccctcccg cagcctcgac acagaactac
    17701 gtcggcgtcg aagtgtcggg cgcgacgctg cgcgcggcgc tcgtctcggc gacgggcgag
    17761 gtgctggggc gacgcgaggc ggcgctggag cgcgacggcg tcgcggcgca ggtcgcgcgc
    17821 gcggcctcgg agctgcgcgg agagtccgaa ggctcgggca cggtcgccgg catcggcatc
    17881 ggactgcccg gcctcgtcaa ccccgcgacc ggcaacgtcg tcatctcgac cgatctgccc
    17941 gccctcgcgc gcggcgacct gcgtaccgag gtggaaaagg ccaccggact gccggcgctg
    18001 ctggagaacg acgccaacgc cgccgcctac ggcgagtacc tcgccggggc ggggcgcggc
    18061 agccgacaca tgttctacat caccatcggc ctcggcatcg gcggcgcctt catcttcgac
    18121 gggcgcatct ggcgcggggc atcgggcctt gccggcgagt tcggccacat caccatcgac
    18181 cccgagggcg aggagtgcgc ctgcggtaac acgggctgtc tggagacggt ggcctccgcc
    18241 cccaacatcg tccgccgcac gcacgaccgg ctcttccgcg acaacacctc gtcgctctcg
    18301 cgtctggcct taaaccgcga cttcgccgcc tccgacatcg cccgcgaggc caccgccggc
    18361 gacgacttcg cccgcctcat gatcgagcgc accggccgct acatcggcac cggcatcgcc
    18421 gccgtcatca acctcctcag cgtcgagcgc atcgtcttag gcggcggcgt gatggaggcc
    18481 ggcgcgctca tcctcgaccc catcatccgc gaggccggcc gacgctcctt ccagccctgc
    18541 ttcgaagcca cacagatcgt cgccgcttcc ctcggccccg acgccgtccc cgtcggcgcc
    18601 gccctcctcg cccgcgccgc ctcctgaagc gccagacaac ttgacggttc agagcgcgtg
    18661 cgccagcagc ctgttgcctg ggctcttttg atgaacctga cgactccgag acgaaaactc
    18721 cctcccgccg ttctgctgtt tgtcctcgcg gcctcggcac tcgcgccggt tggctgtcaa
    18781 aagccgccgc gcgagagtgg cgcgcgcccg cagaagaagg ccacgccgga tcaatcggcg
    18841 ctcttagagc aaatcttcca gaagtacacg gaggcggtgg gcggcgagga agccatcgac
    18901 cgcgtggaga gctacgcgct caagggcgcg ttccagatga cgggcagcga actgacgctg
    18961 cccgtcgaaa tcttcgtcaa gaagccggac atgacgttga tggtgatcga ggtgccgcgc
    19021 gtcggccgga tcagacgcgg ggtgagcgcc gaggggggct gggtccagac gcccttcgcg
    19081 gtcgtctcca ccgaagagag ccccggcgag atcgtcgagg tcgagcggga tcacgatatc
    19141 tatcaggcgg gccggatcag gcgactgtac cgggaggtga aactggaggg tcaggcgcga
    19201 ctcaaggggc gcgacgtgta cgtggtcgag ggcaaggcgg acaaggggcc ggcggaaaag
    19261 atgctcttcg acaaggagac gggcctgctc ctgcgttggg acatcgtgcg gcggcctccg
    19321 ggcaaaccga cggtcttcgc acgggtctac ctcgacgatt acagagatgt cggcggcgtc
    19381 agggtgccct tcacgatccg ctactttttc gagccgcgag agtttaccct ccggctcaac
    19441 gacgtgcggc ataacgtccc gctcgatgat tcgatgttcg ccaagcccgc ggcccgcgcc
    19501 ggggggcgcg attagcctca cccgcgctga gagtcgccgc atttaagcca ttgagttatc
    19561 tcatcgcgcc cggccgttct tctccgaccg ggcgttttat ttatttgaat gtgcgtgcgg
    19621 tttcgcgccc gttttgttat tttgtgacga cgccgctctt gatgccgcgt tagcctcgcg
    19681 cgttcgtgcc gtcataatcg ccgcttgctc tctgcgacca tcccaagcgt gaagaacgtc
    19741 gctgaacttt tcagactcga ctaccgctca ctcgcgctct accgtgtgtt gctgggctgc
    19801 gtgctgctct acgacatcgc ggcgcgcgcg ctggaggtgg cggcgttcta cacggacggg
    19861 ggcgtgctgc cgcgggcgcc gctcgtcgag cagttccaga gcgagtggag ttggagcctg
    19921 cacctgctga gcggggcgtc ggcggtgcag tacgttttgt tcgcgctcgc ggccgtcggc
    19981 gcgctggccc tcatcgtcgg gtacaggacg cgggtcgcga cgctcgtctc gctcgtcctc
    20041 ctgatttctc tccacaaccg gaatccgctg gtgttgagtt cgagcgacca gttgatgcgg
    20101 gtgctgctgt tctggactct gttcctgccc gtgggcgcgg cctactcgtt tgatcgggcg
    20161 cgcaaagggg cggcggcggc ggcgttgtcg cgcccatcga acgcgtccgt gggcacagcc
    20221 gccttcatcc tgcaaatctg tctggtctac tggttcacgg cggcgctcaa gtccgaccgc
    20281 gcgtggtggc acgaggggag cgcgctctac tacgccttca acatcgacta cctgacgacc
    20341 gacctcgggc gcgtgatgct caccttcccc ggcgccttgc gcgcgctctc gtacctctcc
    20401 atctggctgg aggcggtggg gccggcgctg ctcttcttcc cgcgcaaaaa cggcgcgata
    20461 cgaacggcgg tcgtcttcgc gttcatcctc ttccacctgt gcatgactcc tttcctcacg
    20521 ctcggcactt tccccttcgt gtgcgcggcg atgtggagca gcctgctgcc cgcggagttc
    20581 tgggaacgtg tcgggaagaa tttcgggcgt ctcgatgggt gggcggcccg gtcgggcgcg
    20641 ccggcagtcg cgcgcacgcc cgcgtggatg aacgtcgtgg ccggggtctt cttcgtctat
    20701 gtcctcttct ggaatttcgg ccccgtcacg gcgcagcgcg tcggcgtgcc ggcgcgggtc
    20761 ggttggatcg gacaggtcac ggggctggcg caggcttgga ggatgttctc gccggtgccg
    20821 aagaaggacg acggctggta cgtcatcccg gcgaagctca ggggcggcgc cgaggtagac
    20881 ctcttccgca atggcgcgcc cgtcgattgg gccaagcccg actcggtcgt ggcgctctac
    20941 ccgaactctt actggcagaa gtacacggag aacatctggc tgcgcgactt cgccagcgcg
    21001 cgcctccact acgcgcgcta cctctgccgc acctggaacg ccgcgcacgg cggcgacgaa
    21061 gagctacgca ccttcgacat cgtctacatg cgcgaggaca cccccccgcc cggccaaccc
    21121 gtgcccgtcg ccgaacccgt catcatctgg caacacgact gctttaaata agcgcccgct
    21181 cgtcaccggc cgcagtctgg cgcgcgtgcg gtttcgcgcc cgttttgtta tgttaagcgc
    21241 gtgatttcag gcgggattga ttgtgtctgg gcctgattta aggagattca gcagtgatcg
    21301 acgtagagcg actgaagatt caatacgtta ccaatgaggc cggagaaaag acggccgtca
    21361 ttctgtccat tgaagagttc aaagaactct tggaagacat cgaagacctc gcgacggcgg
    21421 ccgagcggcg cgacgagccg acggtcgcgc atgacgacgt ggtcaccgag ttgaagcgcg
    21481 atggactcct ttagagtcga gtggaagcgc tcggccatta aggagctgag aaaccttcca
    21541 agaaacgtcg tagcgcgaat catgcgggcc gtggagcagt tgccggccaa cccgttcccg
    21601 ccgggtgtga gaaagcttgc aggctccgac cacacgtacc gcattcggga gggcgattac
    21661 cgaatcatct acagcgtcgt atcaatgact ctcttaatcg aaatcgttaa agtaggacat
    21721 cgcaaagacg tttacgacag gtaggacgag tttcagtcta tcgttatctt aatgaatcct
    21781 gaacgcatca gaaatttttc gatcatcgcg cacatcgacc acggcaagtc cacgctggcc
    21841 gaccggctgc tgcaactgac gggcgcggtg gccgagcgcg acatggaggc gcaggtcctc
    21901 gacgacatgg acctggagcg cgagcgcggc atcaccatca agtcgcacgc cgtgcgcctc
    21961 gactaccgcg cccgcgacgg acacgactac gtcctcaacc tcatcgacac gccggggcac
    22021 gtcgatttca gctatgaggt gtcgcgctct ctggcggcgt gcgagggggc gctgctcatc
    22081 gtcgatgcct cgcagggcgt ggaagcgcag acgctggcga acacctacct cgccatcgcc
    22141 aacaacctcg aactcatccc cgtcatcaac aagatcgatt tgccgagcgc cgagccggag
    22201 aaggtcaagg agcagatcga gcacgtcatc ggcctcgaca cgcacaacgc cgtgctcgcc
    22261 tcggccaaga ccggcctcgg cgtcccagag attctcgaag ccatcgtccg cgacattcac
    22321 gcgcctgccg gcgaccccga ggcgcccctg aaggccctca tcttcgactc gtggtacgac
    22381 tcctatcgcg gcgtcatcgt cctcttccgc atcatcgacg gccacgtcaa gcccggcatg
    22441 aagattcgct tcttcaacac ggggcgcgac tatcaggtgg agactctggg cgtcaaccgc
    22501 ccccgcccca cgctcatcga ccgcctcggc gtcggcgagg tcggattcct cacggcgtcg
    22561 atcaagacgg tggccgacgt gcagatcggc gacacgatca ccgaggccgc gcggccgacg
    22621 aaggaggcgt ggcccggctt tcaggaaatc aagccgatgg ttttcgcggg cctctacccc
    22681 gtggaagcga accagtatga ggagctgcgc gacgccctgg acaagctgcg cctcaacgac
    22741 gcctcgctct tctacgagcc tgaaagttcc gtcgcgctcg gcttcggctt ccgctgcggc
    22801 ttcctcggcc tcctccacat ggagatcgtg caggagcgtc tggagcgcga gttcgatctg
    22861 gaactcatca cgaccgcgcc gggcgtgcgc taccgcgtga cgacgaccga cggcgaggtg
    22921 atcgaagttg acagtcccgc gaagatgccc gacgccgtcc gcatcgccaa gatcgaggag
    22981 ccggtcatcg aggcgaccat cctctcggcg gacgactacc tcggcggcat cctgcccctg
    23041 ctcgaagaga agcgcggcgt gcagaagaag ttcgagtaca tcgccgacaa gcgcgtgatg
    23101 ctcgtctacg agctgcccct gaacgagatc gtcctcgact tctacgaccg tctcaagtcg
    23161 gtctcccgcg gctacgcctc gctcgattac cacttctccg actactggga ctccgacctc
    23221 gtcaagctcg acctgatggt ggccggcgaa cccgtggacg cgctctccgt catcgtccat
    23281 cgcgccggct cgcaggcgaa gggacgcctc ctggccgaga agatgcgcca gctcatcccg
    23341 cgccagctct tcgaggtgcc cattcaggcc gccatcggca acaaggtcat cgcccgcgag
    23401 accgtcaagg cgctcggcaa gaacgtcatc gccaagtgct acggcggcga catctcgcgc
    23461 aagcgcaagc tcctcgagaa acaaaaagaa ggcaagaagc gcatgaagcg cgtcggccgc
    23521 gtcgaaatcc cgcaggaggc tttcctcgcc gtcctcaagg tcaacgagta gctgaccgct
    23581 gccggcaacg acacgcgtag cacgcccccg gaggtgtaat gagatgacga ccccctcgcc
    23641 aaggcgcgcc gcgggcttcc tgcttcggtg catcgcactg ttctctctgc tgctggcggc
    23701 gtcggcccct tcgtcgcagc tcgcgcgcgc gcaggacccg cgccgtgacg atgacgatga
    23761 ggtgctgcgc gtcagcacca atctcgtgca gacggacgtg acggtgctcg acaagcaggg
    23821 acgcccggtg acagacctcc ggcccgagca gttcgagctg aaggttgacg ggcagacgca
    23881 gccgctcctc ttcttcgagc gcgtgacggc gggcagcgcc ggcgaggcgt cggcgctccg
    23941 gcgttcgacg cgcggcgcaa cgcccacagt ctccacaaca gccgcctcgg cgtcggcgac
    24001 acggccggcg cccccgcgcg gacgctccgt catcttcttc atcgacgacc tccacctcag
    24061 ctcggagggg gtcgcgcgcg cgcgcgacat cctgctcagc tacatcgaac gccgcctcgc
    24121 gcccggcacg ccgacactca tcgcctccac gagcgggcag atcggatttc tccagcagtt
    24181 cacggacgac cgggcggttc tgcgcgcggc cgcgcggcgc gtcaggtatc agtccaccgc
    24241 catcctcgat tttgaccagc cgccgatgtc cgagcgcatc gcgctcgaaa tcgaccgggg
    24301 caaccggggc gcgctcgact actatgtcgt gagctggatc agggacaacc cttcgcgcag
    24361 ccccacccgg cgcgacacgg ccgagcaggt ggttcgcggc cgcgcgcgga cgatagtgga
    24421 gcgcgcggcg caggtgagtg aggccaccat ctcgtcgctc gaatcggcgg tcggcgcgcg
    24481 cgggccggag ccggggcggc gcattctctt cttcgtctcc gaaggcttca cgctcaccaa
    24541 caggaagcgc gacatcggcg acaaataccg gcgcgtgatc gccgcggcgg cgcgggccgg
    24601 gacggtcgtc tacacgctcg acgcgcgcgg tctcgccgtc cccatgctgg acgcctccag
    24661 cggcatgcgc gccgacatcg aacttgaaac tgatctcacc gtcgtcagcc cttcggccga
    24721 catggccgcc cagcaggagg tgctgcgcgg gctggccgca gacaccggcg gcagggcgtt
    24781 gctgaacacc aacgggctgg agcaggccgt cgagagcgcg ctcggcgaaa cggccgagta
    24841 ttacgttctg gcatggcggc ccgaggcggc cgagacggcg ggccgcagtc cgcagttccg
    24901 ccgcgtcgag gtcagtgtgc gcggccgtcc cgacctgacc gtgcgcgtcc gcagcggctt
    24961 tctcgacgcg cgggccgacg acgcacgcca gcgcgtcgcg tccggggccg caggctctgc
    25021 aacgcccgcc gtccgctcgt cgaacgccac gctcagcgcc gtgatcgccg aggcggccga
    25081 gcggcgcgct ctgcccgtgg agctatacgc catcttcaac ggcgagacgg gaagtcctcc
    25141 ctcgctgacg gcgctcgtgc agcttacgga tgagcctgtc aggttcgcgc cggccgggga
    25201 gaagctacag gcggaggtga cggtcgcctg cgtcgtgctc gacgacacgg gcaagaccgt
    25261 ttttagcgaa gggcggacgc tcacgctcac gcgcgcggcc cccggcggcg gcgcgagccg
    25321 cgtcatcact cagtttcccg cgcagctcac gaagcccggt ctgtatcagg tgcgcgtggc
    25381 ggcgcacgac gcgcgcggcg ggctgacggg cagcgccttc cagtgggtcg aggtgcccga
    25441 cctcaaggcg ggtcgcctcg cgctgagcac gccggtcatc gtcgagagac acaccgtcac
    25501 cgccaccacc gcgccggaga cgccgcaggc cgtgtcgttg aatgttgacc gccgcttcgc
    25561 gcgcgcgtcg cgtctgcgtg cggagttgta cgtctacaat gccgcgcgcg cggcgggcgg
    25621 cgcgaacgta gagatggaga tcaaagtctg gcgcgatggc cgggtcgcac tcagcgcacc
    25681 gccgcacaaa ctcgccgatg cgggcgccgc cgacccgtcg cgtatccgtt atgatgcgga
    25741 ggtgccgctg cgcgggcttg cgcccggccc gtacattctg gaagtgagcg cggccgaccg
    25801 cacgacgcgc gcagcggcga cgcgtcagat cgccttcacc gtcgagtaga gacgccgtgc
    25861 gggcgacgct tacgcggccc gcgctattga tgaggtttga aaaatcagga gtgtgcgcga
    25921 tgaaattccc cctggtcgcc ctcgctttgt tgctgtccgc tgacctcgcc tccgcccaac
    25981 accacgcgcc cgcgtcgaag gagcagccgc cgcccgtccg gctgattgcg gaactcggcg
    26041 cctaccgctt ccccatcgcc agaacgcctc acgccgaggc gcagaagttt ttcgatcagg
    26101 ggatgattct cgtctacggg ttcaaccacg acgaggcggc gcgcgccttc cgacgcgccg
    26161 ccgaactcga ccccgcgatg tcgatggccc actggggact cgccctcgcg ctcggcccca
    26221 actacaacga ggcgaccatt tcgcccgagc gcctgcgcgc cgcgcacgac gccgtccgca
    26281 aaggactcgc cctcgccgcc aaggggccgg agcacgaacg cgcctacctc gaagcactcg
    26341 ccaaaagatt ctcgtcggat gcaaatgccg accagaaaaa gctctgggtt gaatataaaa
    26401 acgcgatggc ggacatgacg cggcgctacc cggacgacct cgacgccgct actctgtacg
    26461 ccgacgccgc gatgatcacc aacgcgtgga agctctggag tcccgaaggc aagccggcgg
    26521 aagggacgga agagatcgtc gctgtcctgg agtccgtcat ccaccgcgac cccgagcaca
    26581 tcggcgcgca ccacctctat atccacgccg tcgaagcctc gccgcatccc gagtgggcgc
    26641 tcgcttcggc cgaccgcctc gcggtgttgt cgcccgcggc cggtcatctc gtccacatgc
    26701 ccgcgcacac ctacatgaga gtcggcgact acgagcgcgc ggccctggcc aacgagttcg
    26761 cggcgaaagt tgatcaggag tatttagaca aaggcggcct gcgcggcatc tacacggcgg
    26821 cctactacag ccacaaccta cacttcctcg ccgccgcgcg cagcatgcag ggacgctacg
    26881 cggagtcggt cgcggccgcg cgccggctcg aacagaacgt ccggccgtat ctcaaggaga
    26941 tgcccttctt cgagagcttc ctgccgacgc gctcgctcat cgccgcgcgc ttcggccgct
    27001 gggacgacgt gctgaagatg gagtcgcccg aagcatcggc gcccatcacg aaggcgctct
    27061 ggcactgggc gcgcggcatg gcccacgccg ggactggaaa actttcagac gccgatatcg
    27121 agttgaagac cttcacggcg gccgcgcaag ccatcccggc tgaagcgggc tacggccaga
    27181 acaccgcgcg ctccgtcctc aagatcgccg aacacttcct caactcgcgc atcgccgccg
    27241 cgcgcggcga ccgtaaaagc gccatcgagt tcttacgaca ggcggcggcg gccgaagacg
    27301 ctttgctcta cgacgagccg cccggctggt atcaccctct gtcgcgcgag tcgctgggcg
    27361 gcgcgctcct cttagacggc cagcacgccg aggccgagcg cgtcttccgc gaagacctcc
    27421 ggcgcaaccg acgcaacggc cgctccctct tcggccttgc cgaaagcctc aaggcgcagg
    27481 ggcgcgcgca cgaatcggaa ctcgtccgcc gcgagttcga gcgcgcctgg aagggcgccg
    27541 acacgaagct gcgcgtggaa gacctgtaga ttgcggacta ctcacaacag taccgcgagc
    27601 gatagcgagc gggtgtgcgt actctggcaa gtcctgacct acagtcgcac ccgctcgcta
    27661 ccgctcgcgg tactgagttc gaggtaacga tgaaacgctg gctcctcctg ctcctcctgt
    27721 cggccgcggc gctcgcgctc gtcgctgtgc ccgtgtggct cattcaaccc tttagcccgc
    27781 agacgccgcg cgcgcttgag gtcggctacg cgctgcgccg ttggtcgccg ctcgtcacgc
    27841 tggcgctggc agccggcggg ctagcactca t
//
LOCUS       DASVES010000008        12417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1155307, whole genome shotgun sequence.
ACCESSION   DASVES010000008 DASVES010000000
VERSION     DASVES010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12417
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155307"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..148
                     /locus_tag="VF240_00350"
     CDS             14..148
                     /locus_tag="VF240_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7173718.1"
                     /translation="MCLAILNVLHTELGDPKYRACPLLKRYVDAGWLGRKSGRGFYEY
                     "
     gene            317..1501
                     /locus_tag="VF240_00355"
     CDS             317..1501
                     /locus_tag="VF240_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7173719.1"
                     /translation="MAANSLEQEFCARCGTRLMLVVEPTALRYEEEALDGDYEEHLLE
                     RVSALEGSLARITEKLAQAVEFLTRNARTSYFDHILLETLLGVLGETRVVSQGRVERL
                     WRERFERDAADVAEAGRREELRERVSARYAGGERAAFVQLVGDGFALIEKGREAEGMR
                     HLERAAALSPENALLNSFIGEHFFRTRKMTLARDYLERAIASDPQNPRLCLLLGLVCG
                     DEGDATRARELLRQSLSGGRHTFAAHYALGRLHVAESDWAAALVEFKLALAARKCPEA
                     HYILGLVYYQLGRDRTALRHLRHALGLDADYGEALYLLGLVQLRLGERQSAVESFAAA
                     RSAAGMRALKSPKRAPRLEDMPPPALFREKRGGHKRLLTGGDTRVAAALRDDALSTPA
                     PR"
     gene            1526..1705
                     /locus_tag="VF240_00360"
     CDS             1526..1705
                     /locus_tag="VF240_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173720.1"
                     /translation="MKNKDGQNGPRLAMVEDKATERLKKRAQDLGLIVNAGIGKITEQ
                     ADKKKDGARKEEKRK"
     gene            2017..2538
                     /locus_tag="VF240_00365"
     CDS             2017..2538
                     /locus_tag="VF240_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX7173721.1"
                     /translation="MRIEVEKLSKEGESFAHTYDEGDLILEEGRSRVAGATSVEGHAS
                     RKGEEVCLQGRIRAEVEVSCDRCLRPVAGPLAVEFEESFIPLVADAREGEERELQAAD
                     LHVSVYEGESVDVDELVREQLLLAVPARFVCREDCKGLCPVCGADLNAGDCACPKQET
                     DPRWAALAALKKD"
     gene            2675..2860
                     /gene="rpmF"
                     /locus_tag="VF240_00370"
     CDS             2675..2860
                     /gene="rpmF"
                     /locus_tag="VF240_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7173722.1"
                     /translation="MPNPKSRHSKARTRKRRAHDALRPPQTSLCSNCQEPRMSHRVCP
                     KCGHYKGREVVKVEQEV"
     gene            2907..3977
                     /gene="plsX"
                     /locus_tag="VF240_00375"
     CDS             2907..3977
                     /gene="plsX"
                     /locus_tag="VF240_00375"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX7173723.1"
                     /translation="MRHGQKLTGRVVVDAMGSDHAPHPEIDGALAAARDFGVRVVLVG
                     QPERVLPELRRCGWRKDGDRGIEFVEAEEVIGMDEPVATSVRRKKRSSLRVGAKIVAA
                     GEADGFVSAGNTGAAMATAKMVIGMLPGVDRPALAALLPTKAAKPTLVLDVGANSDCK
                     PHHMVQFAIMGDAYSRAVLGTQRPSVGLMSIGEEEAKGNELTKEAFPLMRDLGSLNFV
                     GNVEGRDVFTGQVDVIVTDGFTGNVMLKLSEGLQDAVLSLIRRELSASTITKAGAVLA
                     RPAFRSLKKRLDYSEYGGAPLLGVRSIVVIGHGSSNARAIRNAIRNVREFSEHGAGER
                     IERGIAETNAREFRGVLGAAGD"
     gene            4007..4993
                     /locus_tag="VF240_00380"
     CDS             4007..4993
                     /locus_tag="VF240_00380"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7173724.1"
                     /translation="MANTNCAILSTGRSYPEGVLTNADLERMVETSDDWITSRTGIKQ
                     RHKAAEGEYTSMFAVRAARQCIERARIDPIDIDLIICATVTPDQILPSTGCIIQTELG
                     CHRAAAFDIAAACSGFLYGLTLANQMVRSGQTRYALVIGAEILTRYVDYTDRQTCVIF
                     GDGAGAALLGPTEDERGILATRIRSDGRYTEQLFSPGGGTRIPPTADTLAEGLHFYKM
                     RGNELFKVAVRSMAEVSREVLEEAGYSADDVKLFIPHQANQRITEAVASKLKVDESIV
                     YSNIEMHGNTSSASIPIALDECVEMGRVREGDLVLMASFGGGVTWGAVLMRW"
     gene            5113..6096
                     /gene="fabD"
                     /locus_tag="VF240_00385"
     CDS             5113..6096
                     /gene="fabD"
                     /locus_tag="VF240_00385"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7173725.1"
                     /translation="MLTATTAFVFPGQGSQAAGMGRDLAQNFPAARAVFEEADAALGF
                     ALSRLCFEGPESELQLTENTQPAILTTSIAALRAMEAEGFPKPDYVAGHSLGEYSALV
                     AAGALTFTAAVRAVRLRGQYMQEAAPVGEGAMAAILGSDLRTIEDACEEAHGGGVCSP
                     ANINAPGQVVIAGTSGAVDRAIEILKTRGAKRAIKLNVSAPFHCALMMPAQERLAADL
                     EQIDFANLGVSIVSNVDAALTRSGVEARAKLIAQVSRTVRWQESVERLAGECGVETFV
                     EVGPGKVLSGLIRKTAQRARCLNVEDGASLRATRAALGEDPKASERAAGGE"
     gene            6183..6434
                     /locus_tag="VF240_00390"
     CDS             6183..6434
                     /locus_tag="VF240_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7173726.1"
                     /translation="MADQSEIETKVKQIIVDELGVDENEVTPNARFIDDLGADSLDTV
                     ELVMRFEEEFGIEIPDEDAEKIQSVRDAYNYIETAKSKQ"
     gene            6610..7866
                     /gene="fabF"
                     /locus_tag="VF240_00395"
     CDS             6610..7866
                     /gene="fabF"
                     /locus_tag="VF240_00395"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7173727.1"
                     /translation="MNRRVVVTGLGLVTPVGHTVKDTWDALLRGQSGVDFIKKFDTEK
                     FSVKFAAEVKDFDPLKWVEKKEARKMGAFIHYAVAAGDEAIYDSGLVERGARLAPDIA
                     DRVGTYISSGIGDFWAIEREHDKLLNGGPGRVSPFFIPSAIVNLAAGQVSIRFGARGY
                     ISATATACSAGAHAIGDSLKVIQRGDADVMICGGAESAITPMSVAGFASMRALSTRND
                     DPVHASRPFDRDRDGFVLGEGAGLLVIEELEFARARGARIYAELVGYGNTADAFHITM
                     PDESASGQLRVMQMAMKDGGVHPEQVGYINAHGTSTPYNDKFETLAIKQAFGEHAYKL
                     AVSSTKSMTGHLLGAAGGIEAVFSVLAIKDGMLPPTINYQTPDPDCDLDYVPNEPREA
                     RLDYALSNSFGFGGTNAALLFKRYEE"
     gene            7966..8745
                     /locus_tag="VF240_00400"
     CDS             7966..8745
                     /locus_tag="VF240_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7173728.1"
                     /translation="MKIVVMMKQVANKDAILRLNKEGTWIEEGDLTFEVNESDGYALE
                     EALRLKDKRGAGEVIVCSMGPARVKSVIKDALARGADRAIHVTGEQLDHIGPFAAARV
                     LADAIREENPDLILTGLQSDDYGYAQTGVIMAEMLGLPHATIVIEVDASGAELRVKRE
                     LESGWYQWYTYPTPALLTIQSGISQIRYATLKGIMAAKKKEIREVAASPDAVGAAGKH
                     QQITKVYFPEKTKQTQFLGDGDAKKGAAQLAEKLRTEARVI"
     gene            8860..9837
                     /locus_tag="VF240_00405"
     CDS             8860..9837
                     /locus_tag="VF240_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX7173729.1"
                     /translation="MPNGILVFVEHRDGAFNKTSYEALAAAQQLGSALGQEVRAVVLG
                     TDASLAGEVAGYHLAGVISAESPKLAEYTPDAYADAMEHVVRAVDPQYVLMPHTYLVR
                     DYSPKLAARFGRDVISDCVRATAEGGKVTFARRIFLGKIDADVAPTDAPPVFATFQSG
                     AYRADQAEKGSGAEVQSMAVEVGEVRMKPEAPFQEVKQAVDLSKADVIVAVGRGIKSQ
                     ENLALAEKLAEALGGELAASRPICDAGWLPIDRQIGSSGQTVAPRLYIALGISGAIQH
                     LVGMKNSTTIVAVNKDAEAPIFDIADYGIVGDLFEAVPVLTEEIKKLKG"
     gene            complement(10067..10354)
                     /locus_tag="VF240_00410"
     CDS             complement(10067..10354)
                     /locus_tag="VF240_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173730.1"
                     /translation="MSARYSVSLRAPFASDWRKSLHADLAAALSSAWRKHSRDWSVGS
                     ITKEQKVVIDRDALEQAFARMDDLLRELPRRPPREAAEQVIREIDREIELG"
     gene            complement(10351..10743)
                     /locus_tag="VF240_00415"
     CDS             complement(10351..10743)
                     /locus_tag="VF240_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173731.1"
                     /translation="MKKLDTGYTFDPHAGATYSFEHMPLADAEERAQVAETPHIVEAV
                     NWTMAEGGEVHYNRYFANSFDEALGLAVRLRIGFEEGVEEVLSIRPATRSEASGGGKA
                     SAHFLELMPLAISSIDKKEGDKKRGGKK"
     gene            complement(10740..10937)
                     /locus_tag="VF240_00420"
     CDS             complement(10740..10937)
                     /locus_tag="VF240_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173732.1"
                     /translation="MTEQEVLELVRRELLEIFEEANTRSRQGSSGGGFGTSAADMSSG
                     KGAPTEYLVQALRERMEGSKK"
     gene            complement(11013..11270)
                     /locus_tag="VF240_00425"
     CDS             complement(11013..11270)
                     /locus_tag="VF240_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7173733.1"
                     /translation="MRIVVEIRVDALLEEHGRSYYWLAKETGISHTTLWRLKKGKALG
                     INFVTLESICLALKCQPGDVLRLAPEKKSSKSRSAKSKHVS"
     gene            11477..11803
                     /locus_tag="VF240_00430"
     CDS             11477..11803
                     /locus_tag="VF240_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173734.1"
                     /translation="MSSLYAMQRANGDWFALDDHGRFRVPVFHSSRDGMLARGRHQGM
                     QLFKPVVLDARALKQFTPAGDAVRDVYFWMVDNPFTTVSRGRSIDHAQLALLVHDGAQ
                     SPAVMT"
     gene            11878..12141
                     /gene="rpoZ"
                     /locus_tag="VF240_00435"
     CDS             11878..12141
                     /gene="rpoZ"
                     /locus_tag="VF240_00435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7173735.1"
                     /translation="MDKTSWNSFSSVTAGGQWPGIDSMYRLIVVAAQRNKQLTRGATP
                     RLAADPRRQRNTSIALEEVKRGLVPFTITEAPTKNDNDASLLL"
BASE COUNT         2321 a         3957 c         4013 g         2126 t
ORIGIN      
        1 catcggcctc gacgtgtgcc tcgccatcct caacgtcctg cacacggaac tgggcgaccc
       61 gaagtataga gcgtgccctc tgctcaaacg ctacgtggac gccggctggc tcggcaggaa
      121 gtccgggcgc ggcttctatg aatactgaaa tgacgtcaat cgtcaatcga ttgcctgtca
      181 ttcgacttgc gattatcagc tcaagttaag cgacactcaa ccacgattga cgattgacga
      241 ttgacgattg acgattcacg gccttacaga tatgcgtacc ccggagaagg caggttcggt
      301 ctactgtcag agatgtttgg cggcgaactc tctggagcag gagttctgcg cgcgctgcgg
      361 gacgcgtctg atgctcgtcg tcgagccgac ggcgctgcgc tacgaggagg aggcgctcga
      421 cggcgactac gaggagcact tgctggagcg cgtctcggcg ctggagggca gcctcgctcg
      481 catcacggaa aagctcgcac aggccgtcga attcctgacg cgcaacgcgc gcacgtctta
      541 cttcgaccac atcctgctcg aaaccctgct cggggtgctc ggcgagacgc gcgtcgtcag
      601 tcaggggcgc gtcgagcgtc tgtggcgcga gcgcttcgag cgcgacgcgg cggacgtggc
      661 cgaggccggc cgtcgcgagg aattgcgcga gcgagtgtcg gcgcgctacg cgggcggcga
      721 gcgcgcggcc ttcgtccaac tcgtcggcga cggttttgcg ctcatcgaga aaggccgcga
      781 ggccgaaggg atgcgtcatc tcgaacgcgc cgccgcgctc tcgcccgaga acgccctgct
      841 caactccttc atcggcgaac acttcttccg cacgcggaag atgacgctgg cgcgcgacta
      901 cctggagcgc gccatagcct ccgacccaca gaatccccgc ctctgccttt tgctcggtct
      961 cgtctgcggc gacgaaggcg acgcgacgcg cgccagagaa ctcctgcggc agtcgctcag
     1021 cggcggccgg cacaccttcg ccgcgcatta cgccttgggc cgcctccacg tcgccgagtc
     1081 cgactgggcg gcggccctcg tcgagttcaa gctcgcgctc gccgcgcgca aatgccccga
     1141 ggcccattac atcctcggtc tggtttatta ccaactcggc cgcgaccgca cggccctgcg
     1201 tcaccttcgt catgcgctgg gactggacgc cgactatggc gaggcgctct atctgctcgg
     1261 gctggtgcaa ctccgtctgg gcgagcgcca gagcgcggtc gaatcgttcg cggcggcgcg
     1321 ctcagccgcc gggatgcgtg ccctcaagtc acctaaaagg gcgccgcgtc tggaggacat
     1381 gccgccgccg gcactcttcc gcgagaagcg cggggggcac aagcggctcc tgaccggcgg
     1441 cgacacacgc gtcgccgccg ccctgcgcga cgacgcgctg agcacgcccg ccccgcgctg
     1501 ataagcagaa agaaaggcgg tagaaatgaa aaataaagac ggacaaaacg ggccgcggct
     1561 cgcgatggtc gaagacaaag cgaccgagag gctgaagaaa cgcgcgcagg acctgggtct
     1621 gatcgtcaac gccggcattg gcaagatcac tgagcaggct gataagaaaa aagacggcgc
     1681 gaggaaagag gagaaaagaa aatgacgctc atcatcgtca ttttctcatc atcctgcgcg
     1741 tcggcgggct tcccacgtgg tgctacagcc ggcgctggcg cggcggcccc gtccccggct
     1801 atggcccgag cggcgtgctc accattatcc tcttgatcat cctcatcctg tggctgatgg
     1861 ggcggctcta gcgcggccgt ccgaagacaa gatggtgaga agtggacacc actgatggca
     1921 gggccggtca tttgacaggc accgcacggc cgctataatt gcaccttttg cgcggagggt
     1981 cgctgacgta gttctttgtc acaaggggag tgagtcatgc gaatcgaggt cgaaaaactg
     2041 tcaaaggagg gcgaaagttt cgctcatacc tacgacgagg gcgacctgat attggaagag
     2101 ggacgctcgc gcgtggcggg cgcgacgagc gtcgagggcc acgccagccg caagggcgaa
     2161 gaggtctgtc tgcaggggcg gattcgcgcc gaggtcgaag tctcgtgcga ccgttgcctc
     2221 cggcccgtcg ccgggccgct cgcggtggag ttcgaggagt cgtttatccc actcgtcgcg
     2281 gacgcgcggg agggtgagga gagggagttg caggcggccg acctgcacgt ctcggtctac
     2341 gagggcgagt cggttgacgt ggacgaactc gtccgcgagc agttgctcct cgccgtgccg
     2401 gcgcgcttcg tctgccgcga agattgcaag ggcctctgcc ccgtctgcgg cgccgacctg
     2461 aatgcgggcg actgcgcgtg cccgaagcag gagaccgacc cgcgctgggc cgcgctggcc
     2521 gcgctgaaga aagattaaaa gccgtaaatc gtcaatggta aatcgtcaat cgattgctcg
     2581 cctctcgact tacgaattac ctttcaagct gctaaaagct caaacacgat ttaccattta
     2641 cgattcacga ttgacgaatt attaaggagt aatcatgcct aatccgaaaa gccgccactc
     2701 gaaggcgcgc acccgcaagc gccgggcgca cgacgcgctc cggccgccgc agacttcgct
     2761 ctgctcgaac tgccaggagc cgcggatgtc gcaccgcgtc tgcccgaagt gcggccacta
     2821 caagggccgc gaggtcgtca aggtcgagca ggaagtttag ggcggactgg tctcaggcgc
     2881 gggcaccggg cgtcaggcgc acttttgtga gacacggaca aaaactgacc ggccgggtcg
     2941 tcgttgacgc gatgggcagc gaccacgcgc cgcaccccga gatcgacggc gcgctcgccg
     3001 ccgcgcgcga cttcggagtg cgcgtcgtcc tcgtcggcca gcccgagcgc gtgctgcccg
     3061 agctgcgccg ctgcggttgg cgcaaagatg gggatcgcgg catcgagttc gtcgaggctg
     3121 aggaagtcat cgggatggac gagccggtgg ccacgtccgt gcggcgcaag aagcgaagct
     3181 ctctgcgggt cggggcgaag atcgtggcgg cgggcgaggc cgacggtttc gtctcggccg
     3241 gcaacaccgg cgcggcgatg gccacggcca agatggtcat cgggatgctg cccggtgttg
     3301 accgcccggc gctggccgcg ctcctgccga cgaaggccgc caagccgacg ctcgtcttag
     3361 acgtgggcgc aaactccgac tgcaagccgc atcacatggt gcagtttgct atcatgggcg
     3421 acgcctactc gcgcgcggtg ttggggacgc agcggcccag cgtggggttg atgagcatcg
     3481 gcgaggaaga ggccaagggc aacgagttga cgaaggaagc cttcccgctc atgcgcgacc
     3541 tcgggagtct taacttcgtc ggcaacgtcg aggggcgcga cgtgttcacg ggacaggtgg
     3601 acgtgatcgt cacggacggc ttcacgggca acgtcatgct caagctgtcc gaagggttgc
     3661 aggacgccgt gctctctttg attcgtcggg agctatccgc ctcgaccatc acgaaggcgg
     3721 gggcggtgct ggcgcggccc gcgtttcgga gtttgaagaa gcgtctggac tactccgagt
     3781 acggcggagc gccgcttttg ggcgtgcgga gcatcgtcgt catcggccac gggagttcaa
     3841 acgcgcgcgc catccgcaac gccattcgca acgtccggga gttctccgag cacggggcgg
     3901 gcgagcgcat cgagcgcggc atcgcggaga cgaacgcgcg cgagttcagg ggcgtgctcg
     3961 gggccgcggg cgactgaagg cagaagcggc gcgagaaggg aatccgatgg cgaacaccaa
     4021 ctgcgcgatc ctgagcacgg ggcgctccta tccggagggc gtgctgacga acgccgacct
     4081 cgaacggatg gtcgagacct ccgacgactg gatcacttcg cgcaccggca tcaagcagcg
     4141 ccacaaggcg gccgaaggcg agtacacctc gatgttcgcg gtgcgcgccg cgcgccagtg
     4201 catcgagcgc gcgcgcatcg accccatcga catcgacctc atcatctgcg ccaccgtcac
     4261 ccccgaccag attctcccct ccacgggctg catcatccag accgagttgg ggtgccaccg
     4321 cgccgcggcc ttcgacatcg ccgccgcctg ctccggcttc ctctacgggc tgacgctcgc
     4381 caaccagatg gtgcgctccg ggcagacgcg ctacgcgctt gtcatcggcg cggagattct
     4441 gacgcgctac gtcgattaca ccgaccggca gacctgcgtc atcttcggcg acggcgccgg
     4501 ggcggcgctc ctcggtccca ccgaggacga gcgcggcatc ctcgccacgc gtatccgctc
     4561 cgacgggcgc tataccgaac aactcttctc gcccggcggc ggcacgcgca tcccgcccac
     4621 ggccgacacg ctcgccgagg gcctgcactt ctacaagatg cgcggcaacg aactgttcaa
     4681 ggtcgccgtg cgctcgatgg ccgaggtctc gcgtgaggtg ctcgaagagg ccggctactc
     4741 agccgacgat gtaaagctct tcattccgca tcaggccaac cagcgcatca ccgaggccgt
     4801 cgccagcaag ctcaaggtgg acgagtcaat cgtctactcg aacatcgaga tgcacggcaa
     4861 cacctcctcg gcctccatcc ccatcgcgct cgacgagtgc gtcgagatgg gccgcgtccg
     4921 agagggcgac ctcgtgctca tggcctcctt cggcggcggc gtcacctggg gcgcggtgct
     4981 gatgcgttgg tgaggaagcg attagctgtt agctgttagc tttcagctta aagaaaccaa
     5041 acgacagcgt ctctattgtt gagcaactac gtggcccacg gcgcatgccg tgggctaaga
     5101 gctaacagct aagtgctaac agctactact gcattcgtct ttccggggca aggctcgcag
     5161 gcagcgggga tggggcgcga tctggcgcag aacttcccgg cggcccgcgc ggtctttgag
     5221 gaggccgacg cggcgctcgg cttcgcgctc tcacggctct gtttcgaggg gccggagagt
     5281 gagttgcagc tcacggagaa cacgcagccg gcgatactga ccacgtcgat tgcggcgctg
     5341 cgggcgatgg aggcggaagg gtttccgaag ccggactacg tcgccggcca cagtcttggc
     5401 gaatactcgg ctctggtcgc ggcgggcgcg ctgacgttta cggcggcggt gcgcgccgtg
     5461 cgactgcgcg ggcagtatat gcaggaggcg gcgcccgtgg gcgagggggc gatggcggcc
     5521 atcctcggct ccgatctgcg gacgattgag gacgcgtgcg aggaagcgca cggcggcggc
     5581 gtctgctccc cggcgaacat caacgcgccc ggtcaggtcg tcatcgccgg cacgtccggg
     5641 gccgttgacc gcgccatcga gatattgaag acgcgcggcg cgaagcgtgc gatcaagttg
     5701 aacgtgagcg cgcccttcca ctgcgcgctg atgatgccgg cgcaggagcg cctcgcggcc
     5761 gaccttgaac agatcgattt cgcgaacctc ggcgtgtcca tcgtctcgaa cgtcgatgcc
     5821 gcgctcacgc gctccggcgt ggaggcgcgc gcgaaactca tcgcgcaggt ctcgcggacg
     5881 gtgcgctggc aggagtcggt cgagcggctg gcgggcgagt gcggcgtcga gacattcgtc
     5941 gaggtcggcc ccggcaaggt cttgtcggga ctgatcagga agacggccca acgcgcgcgc
     6001 tgtctgaacg tcgaggacgg cgcaagcctt cgggcgacgc gtgcggcgct cggcgaagac
     6061 ccgaaggcga gcgagcgcgc ggccgggggc gagtgatttt tgtgcggcgc gcgtggcccg
     6121 gcgggggtcg tgagcgcgcc ggaagttttt tcatcctcaa ctgacggtaa ggagcaggtg
     6181 taatggccga tcaatcggag atcgaaacta aagttaagca gatcattgtg gacgaactcg
     6241 gcgtcgatga gaacgaggtg acgcccaacg cgcgcttcat cgacgacctc ggggccgact
     6301 cgctcgacac ggtcgaactc gtcatgcgct tcgaggagga gttcggcatc gagataccgg
     6361 acgaggacgc cgagaagatt cagagcgtcc gcgacgccta caactacatc gagacggcga
     6421 agagcaagca gtaagtgcca cggccgcccg gcgggcctcg gctgccgcgg cctgtgggcc
     6481 ggggcgccga catgaaggcg gcgcttcggc gcgggcgtga aaaagcgccc gccgcacgca
     6541 cgcgcccgct cggcccgaag cgtcggggcg ggctcgggcg agggaagtac ccgagggagg
     6601 aataagcatt tgaaccggcg tgtagttgtc accggtctcg gcctcgtcac accggtcggg
     6661 catacggtca aagatacctg ggacgccttg ttgcgtggcc agagcggcgt ggactttatc
     6721 aagaagttcg acaccgaaaa gttctccgtg aagttcgccg ccgaggtcaa ggacttcgac
     6781 ccgctcaagt gggtggagaa gaaggaggcg cgcaagatgg gcgccttcat ccactacgcc
     6841 gtggcggccg gcgacgaggc catttacgac tcggggctgg tcgagcgcgg agcgcggctc
     6901 gcgcccgaca tcgccgaccg cgtcggcacc tacatcagca gcggcatcgg cgacttctgg
     6961 gccatcgagc gcgagcacga caagctcttg aacggggggc cgggccgcgt ctcgcccttc
     7021 ttcatccctt cggccatcgt caacctcgcc gccggacagg tctcgatccg cttcggcgcg
     7081 cgcggctata tctcggccac cgccacggcc tgctcggccg gcgcccacgc catcggcgac
     7141 agcctcaagg tcatccagcg cggcgacgcc gacgtgatga tttgcggcgg cgcggagtcg
     7201 gccatcacgc cgatgtcggt cgccggtttc gcctccatgc gtgccctctc gacccgcaac
     7261 gacgaccccg tccacgcctc ccgacccttc gaccgcgacc gcgacggctt cgtcctcggc
     7321 gaaggcgcgg ggctgttggt gatcgaggaa ctggagttcg cccgcgcgcg cggcgcacgg
     7381 atttacgccg agctggtcgg ctacggcaac acggccgacg ccttccacat cacgatgcct
     7441 gacgagtcgg cctcgggcca gctccgcgtc atgcagatgg cgatgaaaga cggcggcgtc
     7501 catcccgaac aggtcggcta catcaacgcc cacggcactt ccactccgta caacgacaag
     7561 ttcgagacgc tcgccatcaa gcaagctttc ggcgagcacg catacaagtt ggccgtcagc
     7621 tccacgaagt cgatgacggg ccacctgctc ggcgcggccg gcggcatcga ggccgtcttc
     7681 tccgtcctcg ccatcaaaga cgggatgctc ccgccgacga tcaactacca gacgcccgac
     7741 cccgactgcg acctcgacta cgtccccaac gagccgcgcg aggcccgcct cgattacgcg
     7801 ctctcaaaca gcttcggctt cggcggcacc aacgccgcgc tgttattcaa acgctacgag
     7861 gaatgatgaa aaggagtcag gagtcaggag tcaggagtca gaataaaact tgcactacct
     7921 tcttcattct gactcctgac tcctgactcc tgaattcgtg gaattatgaa aatcgtcgtg
     7981 atgatgaagc aggtcgccaa caaggacgcg attctgcgtc tcaataaaga gggcacgtgg
     8041 atcgaggagg gtgacctgac gttcgaggtc aacgagtcgg acggctacgc gctcgaagag
     8101 gctctgcggc tgaaggataa gaggggcgcg ggcgaagtca tcgtgtgctc gatggggccg
     8161 gcgcgcgtca agagcgtcat caaggacgcg ctcgcccgcg gggccgaccg cgccatccac
     8221 gtcaccggcg aacagctcga ccacatcggc cccttcgccg ccgcgcgcgt gctggccgac
     8281 gccattcgcg aggagaatcc cgacctgatt ctcacgggcc tccagtccga cgactacggc
     8341 tacgcgcaga ccggcgtcat catggccgag atgttggggc tgccgcacgc caccatcgtc
     8401 atcgaggttg acgcttccgg cgcggagctt cgcgtcaagc gcgaactcga gagcggctgg
     8461 tatcagtggt acacctaccc gacgcccgcg ctcctgacca tccagtccgg catcagccag
     8521 attcgctacg ccaccttgaa gggcatcatg gcggccaaga agaaagagat acgcgaggtc
     8581 gccgcctcgc ccgacgccgt cggcgcggcc ggcaagcacc agcaaatcac aaaggtctac
     8641 ttccccgaga agaccaagca gacgcaattc ctcggcgacg gcgacgctaa aaaaggcgcc
     8701 gcccaactcg ccgagaagct acgcacggag gcgagagtaa tttaaaagtg agcagtgagc
     8761 agtgagcagt gagcagtaaa aaacgcgccc gacggctgac gctgactgtt caccatcaac
     8821 tgcttactgc tcactgctca ctgctcactt aatcttatta tgccaaacgg aattctggtt
     8881 ttcgtcgagc accgcgacgg tgctttcaat aagacttctt acgaggcgct ggccgcggcg
     8941 cagcagttgg ggtcggcgct cgggcaggag gtgagggccg tcgtgctcgg cacggatgcg
     9001 tcgctcgcgg gcgaggtcgc cggctaccat ctagccggcg tcatctcggc cgaaagcccg
     9061 aagcttgcgg agtacacgcc ggacgcctac gccgacgcga tggagcatgt tgttcgcgcc
     9121 gtcgatccgc agtacgtcct catgccgcac acgtatctgg tgcgcgacta ctcgccgaag
     9181 ctggccgcgc gcttcgggcg cgacgtaatc agcgactgcg tgcgcgcgac ggccgagggc
     9241 ggcaaggtta catttgcgcg ccgcatcttc ctcggcaaga tcgacgccga cgtggcgccg
     9301 acggacgcgc cgcccgtgtt cgccaccttc cagtcgggcg cgtaccgggc ggatcaggcg
     9361 gagaagggaa gcggggccga agtgcagtcg atggccgtcg aggtgggaga agtaaggatg
     9421 aagccggagg ctccgttcca ggaggtgaag caggcggttg atttgtcgaa ggcggacgtg
     9481 atcgtggcgg tcggccgcgg catcaagagt caggagaacc tcgcgctcgc cgagaagttg
     9541 gccgaggcgc tcggcggtga actcgccgcc tcgcgtccca tctgcgacgc cggctggctc
     9601 cccatcgacc gacagatcgg ctcctccggc cagacggtcg cgccccgtct ctacatcgcg
     9661 ctcggcatct ccggcgccat ccagcatctc gtcgggatga agaactcgac gaccatcgtc
     9721 gccgtcaaca aagacgccga ggccccgatc ttcgacatcg ccgactacgg catcgtcggc
     9781 gatctcttcg aggccgtccc cgtgctcacg gaggaaatca agaagctgaa aggttgatga
     9841 gcgcgccgtc ggttcgatag ccaatagccc cgtccacacg gacggggcta tttttattgt
     9901 caggcttgat ggttaaactg cttactaacc tatggcgagt tgcgattgcg tgcgccctcc
     9961 ctcacggtcg ggcttgcggc cccggatgtg cctcaaggcg gaagcccgac cgtgagggag
    10021 ggcgtgcgga ggtcatgctc aaatgcaatc cgctataaac tgcctgctaa cctaattcaa
    10081 tctccctgtc tatctcccgg atgacctgct ccgccgcctc gcgcgggggg cggcgcggca
    10141 gctcgcgcag gaggtcgtcc atccgggcga atgcctgctc cagagcgtcc ctgtcgatga
    10201 cgaccttctg ctccttcgtg atgctgccga ccgaccagtc gcggctgtgc ttgcgccacg
    10261 ccgacgacag ggcggcggcc agatcggcgt gaagtgattt gcgccagtcg gaggcgaagg
    10321 gggcgcgcag gctgacggag tacctcgcac tcacttcttg ccgcctctct tcttgtcgcc
    10381 ttccttcttg tcgatgctcg atatcgccag cggcatcaat tcgagaaagt gcgcggaggc
    10441 tttgccgcct cccgacgcct cactccgggt ggccgggcgg atggataaaa cctcttcgac
    10501 gccctcctcg aagccgatcc tgagcctgac ggcgaggccc agcgcttcgt caaaactgtt
    10561 ggcgaagtaa cggttataat gcacctcgcc gccttcggcc atcgtccagt tgaccgcctc
    10621 gacgatgtgc ggggtctcgg ccacctgcgc cctctcctcg gcgtcggcga gcggcatgtg
    10681 ctcgaagctg taggtcgccc cggcgtgagg atcgaacgtg tagccagtgt ctagtttttt
    10741 cacttcttgc ttccctccat ccgctcgcgc aatgcctgaa ccagatattc cgtgggcgct
    10801 cccttgccgg aggacatgtc cgccgccgac gtgccaaagc cgccgccgct gctgccctgc
    10861 ctgctccggg tgttggcctc ctcgaagatt tcgagcagct ctcgcctgac cagttccaaa
    10921 acttcttgct ccgtcatctc ttcacctttc ttcgcggccc gcttcgtcgg cccatcggcc
    10981 gactctctac aaacttcggc gcggcccaac tgttatgaaa cgtgcttact tttcgcgctc
    11041 cggctcttgc tcgacttctt ctctggcgcc agcctcagca cgtcgccggg ctgacatttt
    11101 agagcaagac agatgctctc caaagtaacg aagttgatcc cgagcgcctt gcccttcttc
    11161 aaccgccaca gcgtcgtgtg gctgatgccc gtctcttttg ccagccaata gtaagaccgc
    11221 ccgtgctctt ccaggagcgc gtcaactcga atctcaacca caattcgcat cctccctccg
    11281 gcattcgtca tccatcctgt ttgagtattt accacatgcg agaagtattg tctatgagat
    11341 ataccgcgga tttgacatat atcctacatc taaaatataa tcgcggggaa tcgcgtccgg
    11401 cgaagatacc cgcaccgttg accgaagtta cgtgaaagag tgcggccgcc ggccgtcgtg
    11461 atgaggggaa caggcgatga gcagtctata tgccatgcag cgcgccaacg gcgactggtt
    11521 cgcgctcgac gatcacggac gttttcgcgt gccggtgttt cacagcagcc gggacgggat
    11581 gctggcgcgc ggccgccatc aggggatgca gctcttcaag ccggtagtgc tcgacgctcg
    11641 cgcgctcaag cagttcacgc cggccgggga cgccgttcgc gatgtgtact tctggatggt
    11701 ggataatcca ttcaccactg tgagccgcgg ccggtcaatc gatcacgcgc agcttgccct
    11761 gctcgtacac gacggcgcgc aaagccctgc cgtgatgacg tagaagctga agacgagggg
    11821 ccgcccctgt cacacacttt ttcaccgcgg ccgggatgcg gagaggcaaa acacctgatg
    11881 gataaaactt cgtggaacag ttttagcagc gtcacggccg ggggccagtg gccgggaata
    11941 gattcgatgt accggctgat cgttgtggcg gctcaaagaa acaagcagct tacacggggc
    12001 gcaactccac ggctcgccgc cgacccgcgg aggcaaagaa acacctccat cgcgctcgaa
    12061 gaggtcaaac gtggtctcgt gcctttcacg atcaccgagg ctccgaccaa gaatgacaat
    12121 gacgcgagct tgcttttgta aggtcatcga acaccacggc gcggtgcgct gacacgtcaa
    12181 ccaagtgaaa caccatgctg aagagtcgcc ccgacccatc agtaacagat gtgaaggcgg
    12241 gcgaaactgt tggtgcggga ggaagattat gagcagcagc agaacggtgg aatcatttac
    12301 cgccaaattc gtcgagggcg aaccaaacgt ccgtttggaa ttgatgaata cgactgatgt
    12361 gacactcaag tgcgtcgagg tgctgaccgt ctttctgaag gacgaagaga ctccggg
//
LOCUS       DASVES010000009         9337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_578209, whole genome shotgun sequence.
ACCESSION   DASVES010000009 DASVES010000000
VERSION     DASVES010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578209"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VF240_00440"
     CDS             <1..1431
                     /locus_tag="VF240_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEX7173736.1"
                     /translation="VLLTRRAALKKPVLAFAGVMQTVPSLALFGFLIPLPLLGIGART
                     ATVALILYALLPIIRNTVAGIEGVERSVREAAVAMGMTDAQILRQVELPLATPVILAG
                     VRVATVITVGVATVAAFIGAGGLGTLIYRGLRQYDNALILAGAIPAALMALALDWLIG
                     RVEARMDAGARRASAARAGSVGLRRAAWAAGAVLVVLAGGYAAWRASARADLAVGSKD
                     FTESIILAEVLSQMLEERGLRVERKFELGGNLPHDSLLAGEIDAYPEYTGTSYTAILK
                     HAPETDPRAVYEQVRREYAEQFGVEVGAPLGFENTFAILVRGEEARRLNLRTVSDAVA
                     HAPRWRAGFGQDFMSRADGYEGFARAYGLRFDARPREMDLTLTYRALADRQVDIIAGN
                     STDGMIGALDLAQLEDDRRYFPPYEAVLLFRRDTLARHTQAREVLARLAGSINTEEMR
                     RLNNEVDERKRPIPEVVREWRREKGL"
     gene            complement(1534..1929)
                     /locus_tag="VF240_00445"
     CDS             complement(1534..1929)
                     /locus_tag="VF240_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7173737.1"
                     /translation="MRKLFLLTLLVLVSATGAFAQAGSSQSGDDIPNMGRAPREPNGI
                     GRLDLRVVDEQGNPVQGVRADLRSNRTDGFTCESWNWTDARGVSVLPPLHMGRLTLTL
                     KAKGFATQKVQVLTNSLDQPVRVTMIRKG"
     regulatory      complement(2219..2319)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2589..2861
                     /locus_tag="VF240_00450"
     CDS             2589..2861
                     /locus_tag="VF240_00450"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7173738.1"
                     /translation="MTLIDLLVLLLIAGLCGALGQAISGYSRGGCLVAIALGFIGALL
                     GGWIARAAGLPEIFPVSVGGMNFPIVWSIIGAALFVAIIGLLTRRR"
     gene            3106..4827
                     /locus_tag="VF240_00455"
     CDS             3106..4827
                     /locus_tag="VF240_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173739.1"
                     /translation="MSATRQARYSEVRRPQGAPDLNHFRRRYPLTVAALGALPEREAW
                     LGRIWEEEARWRESSRVVGVAAEVIVRGGPPAGSEVEGEFEVVCAGGGLGLLYAAVLG
                     ARHGRRVLVFDEGEVGRAARVWNVSGEDLTGLERAGLFTREEIEGAVANRRRAGFVKF
                     HDAASRVKAERLWVEGVLDVALDADRLLQLASARLRARRGCATRDRLRFVRAYVEPQR
                     VTVETEDARGRRQLFAARLFIDATGAASPVARQINDGRAPTHVCPTVGTIARGYSRGD
                     APDAVDFSVGELLVSTEDASQHRQLVWGGFAGAPAVDEFTSHLFFYDATESPADKSLL
                     ELFENYFERLPAYKRRGAHWRVRRPLFGYVPGWRRQGWRGARSKTADERVLLVGRGAG
                     SPLSFGAAGAHLRDLGSVTHLVHLALEADLLDARSLAEVCAQGPRPAPAADLADFLRP
                     TPRSAPASVNETLNAVMAALHHLDECVRRELFLGRASFGTLKNIFGHTAKLYPRIFAR
                     AREHLGARGTVLWLAGLAESAIASRRGRAGAGAPGGDEEEGRQRDAAQEFARHLALYK
                     KGRGAEL"
     gene            complement(4873..5091)
                     /locus_tag="VF240_00460"
     CDS             complement(4873..5091)
                     /locus_tag="VF240_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173740.1"
                     /translation="MADDPIIVRGGSVAVDLSDKFKDNGSGGGRKKYKNQNGRLLSVQ
                     INNETPRTLNPGDTITIVTDDGVGSGTP"
     gene            complement(5125..8124)
                     /locus_tag="VF240_00465"
     CDS             complement(5125..8124)
                     /locus_tag="VF240_00465"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7173741.1"
                     /translation="MIGQTVSHYRILKQIGEGGMGVVYVAEDTRLGRRVAVKFPLAGP
                     NEGHYRARFLREARAVSSLNHPHIASVYDFGETDAGQPYIVMELVAGHSLAELLAEGM
                     TLGRAVEIIQDVAAALGEAHRRGVVHRDIKPTNIHVNESGQVKVLDFGLAKHIEETAL
                     AGDDGAAATHLRTRSDVVIGTPLYLSPEQARGRPVDARSDLFSLGALLYECVAGRPPF
                     SGSNIIEIGAQVLHVEPPPPSRMNSRVPAELDRVTLKALAKRPEERFQSADEMIAALE
                     SVRLRLSGNDAARTRRLPPGEADMLRASALVTISRTLNRPRFSPLALIAALFIALGGV
                     WAYAYWRAPSAHRPSVEAQDFYERGVAALRDGAYHRASLALSEAVSRDDRFALAYARL
                     AEAWAELDSLDRAKDELLRLPSLVPDRAALSEIDALYLDAITATVSREYKSAVESYEK
                     IAKLRPDAPEVYVDQGRAHEKDGETAKAIASYTEAANRDSNYATAYLRLGVLHGRLQN
                     LPAALGAFERAEKIYAARGDAEGQAEVFQQRGFLYDSTGRVADARHEFERALELAEAS
                     GNQFQRINALLGLSAVAFEENNVARSQEYVREAIEAAQANGMDNLSAQGLVELGSLHF
                     GRSDYAEAEKYYKQAIEYARRSKARRAEATALINLGSLRIQQSKPDEAISFVNQALSF
                     YRQGNYRKNVSQGLLMLGRANVLKGDYDAALRAFEEQLQLAEQNGDQSQAATSHEGIG
                     RVLTIQEQYPDALTHFQQKYAIGKSSGDQKGTGYGLTERGSVLWQLGRYDDARASLQE
                     AAALADRPDGGYKQLLVYVRVTNAELALSERKFSEARTQAERALDLAGTKYPDDVAEA
                     KRVLGLALAFSGARREGLRLCQEALDTAESLNNLYLISKATLALAQAMLEGGDAAGAL
                     ANALRARESFAQHGQLASEWRAWAVAAQASRQAGDQQKTREFIAHAADLLAGIRQRWG
                     AEAYESYLARPDTEHHRRQFDELSANH"
     gene            complement(8661..>9337)
                     /locus_tag="VF240_00470"
     CDS             complement(8661..>9337)
                     /locus_tag="VF240_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173742.1"
                     /translation="RGYVWAIYPGFDIFTARPDGKDVRRLTETPGYDAEATMSTDGRR
                     IVFTSMRDGDLDIYTMDADGRNVRRLTDALGYDGGPFFSRDGKHIVYRSFHPKTPEQV
                     ERYKKLLAENVIEPGNFEIWVMDADGRNKRQVTTLGAASFAPYFTPDGRRVIFSSNHP
                     NPRGRNFDLYLVNLDGTGLERVTHDESFDGFPMFSPDGRRLVFASNRNARTRGDTNIF
                     VADWVD"
BASE COUNT         1398 a         3003 c         3278 g         1658 t
ORIGIN      
        1 gtgctgctca cgcgccgcgc cgccttgaag aaacccgtgc tcgccttcgc gggcgtgatg
       61 cagaccgtcc ccagcctcgc cctcttcggc ttcctcattc cgctcccgct gctcggcatc
      121 ggcgcgcgca ccgccaccgt cgccctcatc ctctacgcgc tgctgcccat catccgcaac
      181 acggtcgccg gcatcgaggg cgtcgagcgc agcgtgcgcg aggcggccgt ggcgatgggc
      241 atgacggacg ctcagattct ccggcaggtg gagctgccgc tggccacgcc cgtcatcctc
      301 gcgggcgtcc gcgtggcgac ggtgattacg gtcggcgtgg cgacggtcgc ggccttcatc
      361 ggcgccgggg gattgggcac gctcatctat cgtggcctgc gacagtatga caacgcgctc
      421 atcctcgccg gcgccatccc ggcggcgctg atggccctcg cgctcgactg gctgatcggc
      481 cgcgtcgagg cgcgcatgga cgcgggcgcg cgtcgcgcgt cggcggcgcg cgcgggaagt
      541 gtggggctac ggcgcgcggc gtgggcagcg ggtgcggtgc tcgtggttct ggctggcggt
      601 tacgcggcgt ggcgcgcgtc ggcgcgcgcg gatttggcgg tcgggtcgaa ggatttcacg
      661 gagtcgatta ttctcgcgga ggtgttgtcg cagatgctcg aagagcgggg cctgcgggtc
      721 gagcgcaagt tcgagctggg cggaaacctc ccacacgact cgctgttggc cggtgagatc
      781 gacgcctatc ccgagtacac cggcacctcc tacacggcca tcctcaagca cgcgcccgag
      841 accgacccgc gcgccgtcta cgagcaggtc aggcgcgagt acgcggagca gttcggcgtg
      901 gaggtcggag cgccgctcgg cttcgagaac accttcgcca tcctcgtgcg cggggaggag
      961 gcgcgtcgcc tgaacctgcg cacggtctcc gacgccgtcg cgcacgcccc gcgctggcgc
     1021 gccggcttcg gacaggactt catgtcgcgc gccgacggct acgagggctt cgcgcgcgcc
     1081 tacggcctgc ggttcgacgc gcgcccgcgt gagatggact tgacgctcac ctatcgcgcg
     1141 ctcgccgacc ggcaggtgga catcatcgcc ggcaactcca ccgacggcat gatcggcgcg
     1201 ctcgacctcg cgcagctcga agacgaccgg cgctacttcc cgccctacga ggccgtgctc
     1261 ctcttccgcc gcgacacgct cgcgcgccac acccaagcgc gcgaagtcct cgcacgcctc
     1321 gccggctcca tcaacaccga agagatgcgc cgcctcaaca acgaggtgga cgagcgcaag
     1381 cgccccatcc cggaagtcgt ccgcgagtgg agaagagaga agggactgtg aggaaggcaa
     1441 aagtaaaaag gcaaaaggca aaagtgaaga cgcagacggc acaccgcgtc atcttcactt
     1501 ttgcctttta acttttgcct tttaactttt tatttagccc ttgcggatca tcgtgacgcg
     1561 cacgggctgg tcgaggctgt tggtgaggac ttgaaccttc tgcgtggcga aacccttggc
     1621 cttgagcgtg agcgtgaggc ggcccatatg gagcgggggc aggacggaga cgccgcgggc
     1681 gtcagtccag ttccacgact cgcaggtgaa gccgtcagtg cggttcgacc tgaggtcggc
     1741 gcgcaccccc tggacggggt tgccctgttc gtcaacgacg cgcaggtcga ggcggccgat
     1801 gccgttcggc tcgcgcggcg cgcggcccat gttcgggatg tcgtccccgc tctgcgacga
     1861 ccctgcttgc gcgaacgcgc cggtcgcgga tacgagaacc aagagggtta agagaaacag
     1921 tttacgcatg aaaatattct cctgcttgtc tttattactg acgtcttggt gaaagggagt
     1981 gtcccgacgg gcctcggcgg aagaggggac tttcatcacc gtctggccgt gccagtttag
     2041 gatgaggcgc gtggggcggc agttgcacgc gcggggcttg ccgacggaga tttggcggcg
     2101 ccgctaaacc ttgcgagggc agagcttcgc ggcggcccgg cgggcaaaag gaggggccgc
     2161 gtccccggca gtgttgacga ggcgcggccc gtaatgtctg gtgttttact tgcatcactt
     2221 cggcggtcgc tgccgccgtg cgtccccgcc ggcgggtgac gtttaggcca gcgactttgc
     2281 gccccgaggc tttcgcccgc cgggttgcct ttgtcggtgt ctggcggccc cagctagcgg
     2341 agacgcgctt tcaagaggga caggcgcggc cggcgacact tactgctgcg gtcgtcggag
     2401 ccgcgctgac agtaatgatt atattggccg gtgggggcat ttgaaaagag ttattttccc
     2461 gaccccgcgc cgggcgcgac taactcggcc gaagaagcgg aaacttttct cgtcgcctga
     2521 ctgttaaaac actatccgag accgccgagc cggggcgggc gcggatgaga ccggaggagg
     2581 aacgtcaaat gactttaatc gatctgctcg tgctgctttt gatcgcgggc ctgtgcggcg
     2641 cgctggggca ggccatcagc ggctactcgc gcggcggctg tctggtggcc atcgcgctcg
     2701 gcttcatcgg cgcgctcctc ggcggatgga ttgcgcgcgc ggccgggctg cccgaaatct
     2761 tccccgtcag cgtcggcgga atgaacttcc ccatcgtctg gtcgatcatc ggcgccgccc
     2821 tcttcgtcgc catcatcgga ctgctcacgc gacgcagatg agggggagta agcagtgagc
     2881 agtgagcagt caagacgcag gacactcaac cagcattgac tgctcactgc ttactgctta
     2941 ctgcttactg tctcctcacc cttccgcctc gcgtcctgct tttgctacac tcgcgtgtaa
     3001 agaatctccc ttatttgaag tgacggtctt ggtggttgcg cggcatcgtg gggcgaaaga
     3061 gtctcggggc gcgcgacgcg ggaagagttt cggcaaggtt tttttatgag cgccacccga
     3121 caggccagat attcggaggt gcgtcgcccc cagggcgcgc ccgacctcaa tcactttcgc
     3181 aggcgctacc cgctgacggt ggcggcgctc ggcgcgctgc ccgagcggga ggcgtggctc
     3241 gggcgcatct gggaggagga ggcgcgctgg cgcgagtcgt cgcgagtcgt cggcgtcgcg
     3301 gcagaggtga tcgtgcgcgg cgggccgccg gcggggtcgg aggtggaggg cgagttcgag
     3361 gtggtctgcg ccggcggcgg tctcggactg ctgtacgcgg cggtgctggg cgcgcgccac
     3421 gggcgtcgcg tgctggtctt cgacgagggc gaggtgggcc gcgcggcgcg cgtctggaac
     3481 gtctcgggcg aagacctgac ggggcttgag cgcgccgggc tgtttacgag agaagagatc
     3541 gagggcgcgg tcgcgaaccg ccgccgcgcg gggttcgtca aattccacga cgccgcgtcg
     3601 cgcgtcaagg ccgagcggct gtgggtcgag ggcgtgctcg acgtggcgct cgacgccgac
     3661 cgcctgctgc aactcgcgtc ggcgaggttg cgcgcacggc ggggctgcgc gacgcgcgac
     3721 cgtctgcgct tcgtccgcgc ctacgtcgag ccgcaacgcg tgacggtcga gaccgaggac
     3781 gcgcggggcc gccggcagtt gttcgccgcg cggctcttca tcgacgcgac cggagcggcc
     3841 tcgcccgtcg cgcggcagat caatgacggg cgcgcgccga cacacgtctg cccgacggtc
     3901 gggacaatcg cgcgcggcta ctcgcgcggc gacgcgcccg acgccgtgga cttctccgtg
     3961 ggcgaactcc tcgtcagcac ggaagatgcg agccagcacc ggcaactcgt ctgggggggc
     4021 ttcgccggcg cgcccgcagt tgatgagttc acctcccatc tctttttcta cgacgcgacg
     4081 gagtcgcccg ccgacaagtc tctgctggag ttgtttgaga attacttcga gcgcctgccg
     4141 gcctacaagc ggcgcggggc gcactggaga gtgcggcggc cgctgttcgg ctacgtgccg
     4201 ggctggcgcc ggcagggctg gcgcggcgct cggtcgaaga cggccgacga gcgcgtgctg
     4261 ctcgtcggcc gtggggccgg cagccccctg tcgttcggcg cggcgggcgc gcacctgcgc
     4321 gacctcggca gcgtcacaca tctcgtccac ctcgcgctcg aagccgacct cctcgacgcg
     4381 cgctcgctcg ccgaagtttg cgcgcagggg ccgcgcccgg cccctgccgc cgacctcgcg
     4441 gacttcctgc gaccgacgcc gcgcagcgcc ccggcctcgg tcaacgagac tctgaacgcg
     4501 gtgatggccg cgctgcacca tctggacgag tgcgtgcggc gcgagctgtt tctggggcgg
     4561 gcgagcttcg gaacgctcaa gaatatcttc ggccacacgg ccaagctcta cccgcgcatc
     4621 ttcgcgcgcg cgcgagagca tctcggcgcg cgcggcaccg tgctgtggct ggcgggtctg
     4681 gccgagtcgg ccatcgcctc acgccgcggg cgtgcgggcg cgggcgctcc gggcggcgac
     4741 gaagaagaag gccgccaacg cgacgccgcg caggagttcg cccgccatct cgccctctac
     4801 aaaaagggtc ggggcgcgga actttaagcc ccgcgccccg accgcttacc gtttcggccg
     4861 acgtggtcgg ccctacggcg tgccggaccc aacgccgtcg tcggtgacga ttgtgatcgt
     4921 atcgccgggg ttcaacgtcc gcggcgtttc gttattgatt tgcacgctga gcagtcggcc
     4981 gttctgattc ttgtacttct tacggcctcc gccgctgccg ttgtctttga acttgtctga
     5041 taaatcaacg gcgacggatc cgcctctcac gatgatgggg tcatcggcca tgaaggttgc
     5101 tcctttcaga gtgaggttgc agggttagtg gtttgcagac agttcgtcga actgtctgcg
     5161 gtggtgctcc gtgtccggcc ttgccaggta gctctcgtaa gcttccgcgc cccatcgctg
     5221 ccgtatgcct gccagcaagt cggcggcgtg tgcgataaac tcccgagtct tctgctgatc
     5281 tccggcctgc cggctggctt gcgccgcgac ggcccacgcg cgccactcgg aggcaagctg
     5341 cccatgttgc gcgaaactct ctcgggcgcg gagcgcattc gccagtgcgc ccgcggcgtc
     5401 gccgccctca agcatggcct gtgcgagcgc gagcgtcgcc tttgaaatca ggtaaaggtt
     5461 attcaggctc tcggccgtgt cgagagcctc ttgacacagc cgcagacctt cgcgccgcgc
     5521 gccggagaag gcgagcgcca gccccaaaac gcgcttggcc tcggcgacat cgtccggata
     5581 tttcgtgccc gccagatcga gcgcgcgttc ggcctgcgtc cgggcttccg aaaacttccg
     5641 ctcgctcaac gccagttcgg cgttcgtgac gcgcacgtaa acgagaagtt gcttgtaccc
     5701 gccatcggga cggtctgcca gagcggccgc ctcctgaagc gacgcgcgcg cgtcgtcgta
     5761 gcgccctaac tgccacaaga cgctgccccg ctccgtgagt ccgtagcccg tgcccttctg
     5821 atcgcctgac gacttgccga tggcatactt ctgttggaag tgtgtgagcg cgtccgggta
     5881 ttgctcctga atagtcaaca cccggccgat tccctcgtgt gaggtggccg cctgcgactg
     5941 gtcgccgttc tgttcggcca gttgcagttg ttcctcgaac gcgcggagcg cggcgtcgta
     6001 atcccccttc aatacgttgg cgcgcccgag catgagcagt ccctgcgaga cgttcttgcg
     6061 gtaattgccc tgccggtaaa aggagagcgc ctgattgacg aaactaatcg cctcgtccgg
     6121 cttgctctgc tggatgcgca ggctgccgag gttgatgagc gccgtcgcct ccgcccgccg
     6181 cgctttagac cggcgggcat actcaatcgc ctgcttgtaa tacttctcgg cctcggcgta
     6241 atcagatcgc ccgaaatgga gactgcccaa ctcgaccagg ccttgcgccg acaagttgtc
     6301 catgccgttg gcctgcgccg cctcaatcgc ttcacgcacg tactcctgcg accgcgcgac
     6361 gttgttctcc tcgaacgcga cggcgctcaa cccgaggagg gcgttaatgc gctggaattg
     6421 gttgcccgag gcttcagcca gttcgagagc gcgctcgaac tcgtgccgcg cgtcggcgac
     6481 gcggcccgtg ctgtcgtaga ggaagccgcg ctgttggaac acctccgcct gtccctcggc
     6541 gtcgccccgc gcggcgtaaa tcttctcggc ccgctcgaag gcccccaacg ccgcggggag
     6601 gttctgcaag cgcccgtgga gcacgccgag gcggaggtac gcggtggcgt agttagaatc
     6661 gcggttggcg gcctccgtgt agctggcgat ggccttcgcc gtctcgccgt ccttctcgtg
     6721 ggcgcgcccc tgatcgacgt aaacctcggg cgcgtcgggc cgcagcttcg cgattttttc
     6781 gtaggactcg acagcgcttt tgtactcgcg gctcaccgtg gccgtgattg cgtcgagata
     6841 gagcgcgtca atttccgaga gcgccgcgcg gtctgggacg agcgagggga gccgcagaag
     6901 ttcgtctttg gcgcggtcga gggagtccag ctcggcccac gcttcggcca ggcgcgcgta
     6961 ggcgagggcg aagcggtcgt cgcggctgac ggcctcggag agcgcgaggc tggcgcggtg
     7021 gtaggcgccg tcgcgcagcg ccgccacgcc gcgctcgtag aagtcctgcg cctcgaccga
     7081 ggggcggtgg gccgaggggg cgcgccagta ggcgtaggcc cagacgccgc cgagggcgat
     7141 aaagagggcg gcgatgagcg cgagcggcga gaagcgcggg cggttgagcg tccgggagat
     7201 cgtcacgagg gcgctggcgc ggagcatgtc ggcctcgccg ggcggcagcc ggcgcgtgcg
     7261 cgccgcgtcg ttgccgctga ggcgcaggcg gacgctttcg agcgccgcga tcatctcgtc
     7321 ggccgactgg aaacgctctt cggggcgctt ggcgagcgcc ttgagggtga cgcggtcgag
     7381 ttccgccggc acgcgcgagt tcatgcgcga gggcgggggc ggctcgacgt gcagaacctg
     7441 cgcgccgatc tcgatgatgt tcgagccgga gaagggcggg cggcccgcca cacactcgta
     7501 gaggagcgcg ccgagggaga agaggtcgct gcgcgcatcg acggggcggc cgcgcgcctg
     7561 ctcgggcgag agataaagcg gcgtgccgat gaccacgtcg ctgcgcgtgc ggaggtgggt
     7621 ggcggctgcg ccgtcgtcgc cggcgagcgc cgtctcctcg atgtgcttgg cgaggccgaa
     7681 gtcgagcacc ttcacctggc cgctctcgtt gacgtggatg ttcgtcggct tgatgtcgcg
     7741 gtggacgacg ccgcggcggt gcgcctcgcc gagcgcggcg gccacgtcct gaatgatctc
     7801 gacggcgcga ccgagcgtca tgccctcggc gagcagctcg gcgaggctgt ggcccgcgac
     7861 cagctccatg acgatgtagg gctgcccggc gtcggtctcg ccgaagtcgt agacggaggc
     7921 gatgtgcggg tggttgagcg aggagacggc gcgggcttcg cgtagaaagc gcgcgcggta
     7981 gtggccctcg ttggggccgg cgagcgggaa cttgacggcc acgcggcggc cgagccgcgt
     8041 gtcctcggcg acgtagacca cacccatccc gccctcaccg atctgtttca gaatgcggta
     8101 atgagaaaca gtctgcccaa tcatggggtc ggaggctgac actttaagaa ggagcgagga
     8161 caagaaacta acggaaccgc cggggcgaat tttgagccga cgtggaactt gcgcgcgttc
     8221 ggcccagacg tgaacgctga cgtattctgc tctaaagccg gcggcttgac aagaatcatc
     8281 cgcgccgacg agccgctgaa agcggcggca gagtgtagcc cacggcgtga ggccgtgggt
     8341 aacggacggg caggacgatg gaagccccgg aggggcgaaa gaatttcaac gggcctcgtg
     8401 ctcaacgtcc cgattctttc gcccctccgg ggcttcttat tctaaccgcc tatttacccc
     8461 ggtctcacga ccggggctac actctgccgc cgctccgcgg ctcgtttgac gcccaccctt
     8521 cggcctcctc acttttgcct tttgcctttt acctttttac ttccgtgaga gacaagggat
     8581 gaagaagtgt gagcagtgag cagtcaggac gcttgacctg ctttgactgc tcactgctca
     8641 ctctctcatt catccctcat tcagtccacc caatcggcga cgaagatgtt ggtgtcgccg
     8701 cgcgtgcggg cgttgcggtt cgaggcgaag acgaggcggc ggccgtcggg cgagaacatg
     8761 gggaagccgt cgaagctctc gtcgtgggtg acgcgctcta ggcccgtgcc gtcgaggttg
     8821 acgagataga ggtcgaagtt tcggccgcgc gggttcgggt ggttcgaaga gaagatgacg
     8881 cgccggccgt cgggcgtgaa gtagggcgcg aaactcgccg cgccgagtgt ggtgacctgt
     8941 cgcttgttgc ggccgtcggc gtccatcacc cagatttcaa agttgcccgg ctcgatgacg
     9001 ttctctgcta agagcttctt gtagcgttcg acctgttcgg gcgtcttcgg gtggaaggag
     9061 cgatagacga tgtgcttgcc gtcgcgggag aagaaggggc cgccgtcgta gccgagggcg
     9121 tcggtgagcc ggcggacgtt tcggccgtcg gcgtccatcg tgtagatgtc gaggtcgccg
     9181 tcgcgcatcg aggtgaagac gatccggcgg ccgtccgtgg acatggtcgc ctcggcgtcg
     9241 tagccgggcg tctcagtcag gcgacgcacg tccttgccgt cgggtcgggc ggtgaagatg
     9301 tcgaagccgg ggtagatggc ccagacgtag ccgcgcg
//
LOCUS       DASVES010000010         8789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_957769, whole genome shotgun sequence.
ACCESSION   DASVES010000010 DASVES010000000
VERSION     DASVES010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957769"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1844)
                     /locus_tag="VF240_00475"
     CDS             complement(123..1844)
                     /locus_tag="VF240_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEX7173743.1"
                     /translation="MARLPHGAVIRMEAVREAQEALVGPAAASLAFAASRPIDPHNFD
                     FLFPGLQNNPDNLLPVARRTRDNLIRLGQSMRDTSGGDPAGDSDIPAAYTYLGQFIDH
                     DITLETVSATLPELLDPQLKPLPPGQIKNKIRNLRTAGLDLDSLYGLPAPRQDIKMVV
                     GNVSPTGGTQIPLRRPPGKTDDNDLPRERRSPDIEHDRAALIGDPRNDENTIVAQLHT
                     AFLLAHNVLAGRFQSFMQARRVLRQHYQHMVIQDFLKRVCDPRIVNETVRENHVYDAM
                     AEPFFLPLEFSVAAYRFGHTMVRGAYNFNLNFNTSGAPGTVPATLGLLFTFTALSGQL
                     GGGVVTAQGFDTLPENWIVEWENLVDAGRPFDRTRRLDTKLVEPLFELTDTLGNIIGS
                     DALPPDPNALNGARLAVRNLLRGYLLRMPTGQAVARALKRKLQGVRDIPVLTPAQIVA
                     GAASPEQENALRESGFDGRTPLWYYILAEAAILGRGQRLGPVGSTIVAEVIVGLVRRS
                     EDSILRERGWRPTLPSARRGTFELADLLRLAQRLQSRPSPSGAKKKAGATKKSGAAKS
                     SGAARKR"
     gene            complement(2302..3789)
                     /locus_tag="VF240_00480"
     CDS             complement(2302..3789)
                     /locus_tag="VF240_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX7173744.1"
                     /translation="MLRRFARIAALSLALPLPAAVAQSAQLTTTSKDRRTVNVTVYNS
                     NIGLVRETRAVRLPGQGRIALNFADVAASIRPETVHLVSLTAPEHLSVLEQNYQYDLL
                     NPSKLLDKYVGREVTLLVYRYANNSTVPTPTPVRAVLLSNNNGQVWKIGDEIVVNPSY
                     TEIRFPEVPANLVATPTLVWEVDNTGAAEQTLEASYLTGNMNWKADYVLVIDETDTRG
                     RIQGWVTLTNQSGVGFEDARLQLVAGDLNRVIDRREAEEMAAGVRRATAPSAKDAFQE
                     QAFFEYHLYTLERPATVREQETKQITLLEAADFAASKDYVVNGQPHYYQHYNAPGQPL
                     KEPVGVFVEFRNSRENKLGMPLPAGVIRLYKRDAAGGQQFIGENRIAHTPKDEDVRIK
                     VGDAFDIVSERKQTEYRLVGRNTYEYAYEITVRNHKDTPVEVVFNEPVGESDWKILPA
                     ATTCAGSPRCVGFRAEKTSVTGARLRVPVERDGETKISYRIRVKY"
     gene            4090..4446
                     /locus_tag="VF240_00485"
     CDS             4090..4446
                     /locus_tag="VF240_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX7173745.1"
                     /translation="MTEDLASRHCVPCHGGVPRLRGAEIEPLARQLADWRVADEHHLE
                     KNFEFTNFAGALRFANRVGEVAEREGHHPDICLGWAYAHIRIWTHAIDGLSESDFILA
                     AKIDALTEGEGVKGKG"
     gene            4515..5231
                     /locus_tag="VF240_00490"
     CDS             4515..5231
                     /locus_tag="VF240_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173746.1"
                     /translation="MEVESSFRPEDVEARGAMCVECLCALAAGAAVLDRCGAALCRAC
                     AAEFYAACAGCARLVPRDEGLTRPEDAAGVGALFCVECFRAPAGDAEPPPSDEEVERL
                     VARYVALHAEKKQLDDEMEEIKERLKLAAGARSRVTNAVVLRAGEGAVRCSYSVRTTW
                     DAAKLSEAEALLGPDEFSSLFERKVSYAAVRERLAEFLAGTGDAHTPARELVRAAEQS
                     AETVTLGVVAPKKKKKPADS"
     gene            5545..>8789
                     /locus_tag="VF240_00495"
     CDS             5545..>8789
                     /locus_tag="VF240_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7173747.1"
                     /translation="MTERPLTAQRLAQYVARGRCERHLRFALFPTEALGLLRRYGLEI
                     EPLSPLLSSAGQAFEREQFEELSARARVLDLQGQTPEAFVEELRRLPGAPAGPTFFYQ
                     PSMRAPFGAWACEGRADLVRVERDAATGRVEATVIDIKASRRETVGFRLQVAFYARLL
                     RGALADAGLTPAAIHGAIAARDSELTDDGGWETFDLALYSDEVERLVAAADSDVARAA
                     AAGPHGARYHLRAACDGCPYNQLCFADTAERLDLSLVPHLTISEKRALHAAGVGDARE
                     LAALMEYGEGGAMVVSAGAEARSRVVASRWPLASRLPVLVQRARAAARRFDRATAARR
                     YLVGSDYGSLPDPAQYPDLVRVFVDAQRDYLEDRLYLIGARVTGPRASFEVIEMTDAP
                     PDLDSERALLVAWVQNLLPAVARAAESERAPLHVYTYAARGERTLLDALARHFDALCA
                     IPAFYDLLTSRPALSQGMLSLLSEEVRARLNLPPVCQNLYEVARELGFKWEDGPVDFR
                     KIFRARVFDNRRTFVRDAAAGVFTKDAARAVKEDEAGAVNDDEEDAPPEPHSVRVESA
                     ARFGVEIPLEYAYAAWGRLREPEASATQPASGRRSPLRGFVGVSAEEIRALEGARLRA
                     MQHVEESFAYKNRRVEKEPLDLSGLDRVDVEPEEVPLRRSLEDFLLLEHHARFQDLLL
                     HLALPPRQRAETGRTLMLRCERYLKDEDGERAHFRFADADTNDADGTEADGVVAGDAD
                     ADPPPQQQQQTPLRVRAGDWVVVNPSRDGEGRALPAWRLVRGRLAVVEELSASEVALR
                     LLPMSFKNSPFRYPHVLFKPEPGVVYTADEMADDLNSDKFLEACRNAATNHLYRWLSD
                     MGEGKRARAVRPRRLRDAEAMASLASAAQSPHGLTGAQREIVGGHLCERVLVVQGPPG
                     TGKSHTLGFAILARALAQTTPARPFRVAVLAKTHAAVAVALESIAKRRRELTEAIKGD
                     AATTAHPAAPLLAPLARRRLAKMCNDAGETVPAGVERVLPDGGDESTAAEQWQSLLTE
                     ELLVVGGTPGGLYRLVKLGASKGRAVDWAAEHFDLVVVDEASQMGLAEALTA"
BASE COUNT         1282 a         2866 c         3202 g         1439 t
ORIGIN      
        1 cgcccggcgc gcgccgtaca gacgaatgcc cgccgcgtcg ccgctgcttc ggcgacgcgg
       61 cgggcattgc tgattcgtcg gccgcgacgc gggccgcggc gacgtgtgag agtgaaggcc
      121 cgttaacgct tcctggcggc accgctgctc ttagccgcac cgctcttctt cgtcgcgccg
      181 gccttcttct tcgcgccgga ggggctgggg cggctctgta gacgctgggc gagccgcagc
      241 aggtcggcca gctcgaaggt gccccgcctc gcggatggca gcgtcggccg ccaaccgcgt
      301 tcgcgcagga tcgagtcctc gctgcgccgc accagcccga cgatgacttc cgcgacgatg
      361 gtgctgccga cggggccgag gcgctgtccc cgcccgagga tggccgcctc ggcgaggatg
      421 tagtaccaga gcggcgtgcg gccgtcgaac ccggactcgc gcagggcgtt ttcctgctcg
      481 gggctggccg cgcccgccac gatctgcgcc ggggtgagga cggggatgtc gcgcacgcct
      541 tgcagcttcc gcttgagagc gcgcgccacg gcctgccccg tcggcatccg cagcaggtag
      601 ccgcgcagga ggttgcgcac ggcgaggcgc gcgccgttga gggcgttcgg gtcgggcggc
      661 agggcgtcgc tgccgatgat attgccgagc gtgtcggtca gctcgaagag cggctcgacg
      721 agcttcgtgt cgagtcggcg cgtgcggtcg aaggggcggc ccgcgtcaac gaggttctcc
      781 cactcgacga tccagttctc gggcagcgtg tcgaagccct gcgcggtgac gacgccgccc
      841 ccgagctggc cgctcagggc ggtgaaggtg aagagcaggc cgagcgtcgc cgggacggtg
      901 cccggcgcgc cgctcgtgtt gaagttgagg ttgaagttgt aggcgccgcg caccatcgtg
      961 tggccgaagc ggtaggccgc caccgagaac tccagcggca ggaagaacgg ctcggccatc
     1021 gcgtcgtaga cgtggttctc cctgaccgtc tcgttgacga tgcgcgggtc gcagacgcgc
     1081 ttcaggaagt cttggatgac catgtgctgg tagtgctggc gcagcacgcg tcgcgcctgc
     1141 atgaaggact ggaaccgtcc ggccagcacg ttgtgggcga gcaggaaggc cgtgtgcaac
     1201 tgcgcgacga tggtgttctc gtcgttgcgc gggtcgccga tgagcgcggc gcggtcgtgc
     1261 tcgatgtcgg ggctgcgccg ctcgcgcggg aggtcgttgt cgtcagtctt gcccggcggg
     1321 cggcgcagcg gaatctgggt gccgcccgtg ggggagacgt tgccgaccac catcttgatg
     1381 tcttggcgcg gggccggcag gccgtagagg ctgtcgaggt cgagacccgc cgtgcggagg
     1441 ttgcgtatct tattcttgat ctggcccggc gggagcggct tgagctgggg gtcgagcagt
     1501 tcgggcagcg tggccgagac ggtttcgagc gtgatgtcgt ggtcgatgaa ctgcccgagg
     1561 taggtgtagg cggccgggat gtcggagtcg ccggccgggt cgccgccgct cgtgtcgcgc
     1621 atcgactggc cgaggcggat gaggttgtcg cgcgtccggc gcgcgacggg caggaggttg
     1681 tcggggttgt tttgcagccc cggaaagagg aagtcgaagt tgtgcgggtc gatgggtcgc
     1741 gacgcggcga aggccagaga ggccgcggcc ggaccgacca gcgcctcctg cgcttcgcgc
     1801 accgcttcca tcctgatgac ggccccgtga ggtaaacgtg ccatgtaaga ttcctccagt
     1861 cgtagtgttg gtgagtggcg ccggaaccgg cgggtggccg cgcgtgcgag tgtgtcgcgg
     1921 cggccgggtc gtgcctttct ccgcgggccg cgccgggcga gacgggcgcg ccgcgcccgc
     1981 ggaaggaatg agtcggcgcg tgcgggtgtg cgcgcgcggg ttgggggctc tttggacggc
     2041 ggcgattata cccgcggcgc ggcgcggcgc aacattattt ttcgccgccg gagacgcgcg
     2101 ccacgtcctt tcgccgcgac gggggccgat tgatttgaaa acgcctcaga aatgtcaaaa
     2161 aggcatgagc cgcgaagtca ggcgaagcgg cacaaaacag atgtcactcc gattcgtggc
     2221 gcttcgcctg tcttcgcggc tcattatccc gcgcccctca ttgaggaggg gtgcttctta
     2281 cgaagcgggc gcgggcggcc gtcagtactt cacccggatg cggtagctga tcttcgtctc
     2341 gccgtcgcgc tcgacgggga cgcgcaggcg cgcgcccgtc acggaggtct tctcggcgcg
     2401 gaagccgacg cagcgcggcg agccggcgca ggtggtggcg gcggggagga tcttccagtc
     2461 gctctcgccg accggctcgt tgaagaccac ttcgacgggc gtgtccttgt ggttgcggac
     2521 ggtgatttcg taggcgtact cgtaagtgtt gcggccgacg agccggtact cggtctgctt
     2581 gcgctcggag acgatgtcga aggcgtcgcc gaccttgata cgcacgtcct cgtccttcgg
     2641 cgtgtgcgcg atccgattct cgccgatgaa ctgctggccc cccgcggcgt cgcgcttgta
     2701 gaggcggatg acgccggccg gcagaggcat cccgagcttg ttctcgcgcg agttgcggaa
     2761 ctcgacgaag acgccgaccg gctccttgag gggctggccc ggcgcgttgt agtgctggta
     2821 atagtggggc tgcccgttga cgacgtagtc cttgctggcc gcgaagtcgg cggcttcgag
     2881 cagcgtgatc tgcttggtct cctgctcgcg caccgtggcc gggcgctcca gcgtgtagag
     2941 gtggtactcg aagaacgcct gttcctggaa ggcgtccttc gccgagggcg cagtcgcgcg
     3001 gcgcacgccc gcggccatct cttcggcttc gcggcggtcg atgacgcggt tgaggtcgcc
     3061 ggcgacgagt tgcaggcgcg cgtcctcgaa gccgacgccg ctctggttgg tgagcgtcac
     3121 ccagccctgg atgcggccgc gcgtgtccgt ctcgtcgatg acgagcacgt agtcggcctt
     3181 ccagttcatg ttgccggtga ggtagctggc ctccagcgtc tgctcggccg cgccggtgtt
     3241 gtccacctcc cagacgagcg tcggcgtggc gacgaggttg gccggcacct cggggaagcg
     3301 aatctcggtg tacgaggggt tgacgacgat ctcgtcgccg atcttccaga cctgcccgtt
     3361 gttgttcgag agcagcacgg ccctgaccgg cgtgggcgtc ggcacagtcg agttgttcgc
     3421 gtagcggtag acgagcagcg tcacctcgcg cccgacgtac ttgtctaaga gcttcgacgg
     3481 gttgagcagg tcgtactggt agttctgttc aagcacgctc aggtgttcgg gcgccgtgag
     3541 cgagacgagg tggacggtct cggggcggat ggacgcggcc acgtcggcga agttgagcgc
     3601 gatgcggccc tggccgggga gacgcacggc gcgcgtctcg cgcacgagtc cgatgttcga
     3661 gttgtagacc gtgacgttga ccgtgcgccg atccttcgac gtggttgtca actgcgcgct
     3721 ctgcgcgacg gcggccggca aggggagggc gagcgacagg gcggcgatac gagcgaagcg
     3781 gcgtagcata tctacgggga acctccggga acgtgatttc gacgggtgga caaaggctcg
     3841 gcgcacgcgc gcgtggcgtc cgcgtttctt tgtctcgcgt gtgaagaacg ccgaatgagg
     3901 cgcgtgcttg cgcgttcggt ttagacgaat gaaaaatctg cggttagaat agcggccgac
     3961 cttctacggc ccggcgcgcg agccgcgccg gcacaaacac tttcaccgac acgacgaacg
     4021 ccgcagccgc agccgcgcca cacgccggcg ccggcgcgcg cgcgcaagac aaacgggagg
     4081 actcatccgt tgacggaaga tttggcgagc cgccactgcg tgccctgcca cggcggcgtg
     4141 ccgcgcctgc gcggcgcgga gatcgagccg ctcgcgcgac agctcgcgga ctggcgggtc
     4201 gccgacgagc atcatctgga gaagaatttc gagttcacga acttcgccgg ggcgctgcgg
     4261 ttcgcgaacc gcgtcggcga agtggccgaa cgcgaaggcc atcaccccga catctgcctc
     4321 ggctgggcct acgcgcacat ccgcatctgg acgcacgcca tcgacggcct cagcgaaagc
     4381 gacttcatcc tcgccgccaa gatcgacgcg ctcacagaag gggaaggggt aaagggtaaa
     4441 gggtaaagga aagcgcacgg ccgaacgccg cctttaccct ttaccccttc ccctttaccc
     4501 gtgattgaga ctttatggaa gtagagagca gtttccggcc cgaggatgtc gaggcgcgcg
     4561 gcgcgatgtg cgtggagtgt ctgtgcgcgc tggcggccgg tgcggccgtt ttggacagat
     4621 gcggggcggc gctgtgccgt gcgtgcgcgg cggagtttta cgcggcgtgc gcgggatgcg
     4681 cgcgtctcgt gccgcgcgac gaggggctga cgcgccccga agacgcggcc ggggtcgggg
     4741 cgctcttctg cgtcgagtgc ttccgcgcgc cggcaggtga cgccgagcct ccgccttcgg
     4801 acgaggaggt ggaacgtctc gtcgcgcgct acgttgcgct gcacgcggag aagaaacaac
     4861 tcgacgacga gatggaggag atcaaggagc gtctgaagct ggcggcgggc gctcgctcgc
     4921 gtgtgacgaa cgccgtcgtg ctgcgggccg gcgagggcgc ggtgcgttgc agctactcgg
     4981 tgcggacgac gtgggatgcg gcgaaactct cggaggccga ggcgctgctc ggccccgacg
     5041 aattctcctc gctcttcgaa cgcaaggtgt cttacgcggc agtccgtgag cgcctcgcgg
     5101 aattcctcgc cggcacgggc gacgcgcaca ctcccgcgcg cgaactcgtg cgcgccgccg
     5161 agcagtcggc cgagacggtg acgctcggcg tcgtcgcgcc gaagaagaaa aagaagccgg
     5221 cggactcgta gcgacgacgc gcctccgcgc cacaacctga gcgccggagg cgcggaagaa
     5281 tgtagcccac ggcgcgagcc gtgggaacaa ggcgattaaa ggagtgaagc cccggagggg
     5341 cgaaagaatc aggatgttag ttgccgtcag cgtcgaaatt ctttcgcccc tccggggctt
     5401 tcatcttcca ctgccattga cccacggcta acgccgtagg caacactgct gccgccgctt
     5461 acagcggctc gttgacgctc tcacttccgg tctggctcgc aacctcgtat gtcgtccaac
     5521 actacagaac ctctcgcaga ctttgtgacc gaacggccgc ttacggcgca acgtctcgcg
     5581 cagtacgtcg cccgcgggcg ctgcgagcgt cacctgcgct tcgcgctctt cccgacggag
     5641 gcgctgggac tgctgcggcg ctacgggctg gagatcgagc cgctgtcgcc gctgctttca
     5701 agcgccggtc aggcgttcga gcgcgagcag ttcgaggaat tgtcggcgcg cgcgcgcgtg
     5761 ttggacttgc aggggcagac gccggaggcg tttgtcgagg agctgagacg actgcccggt
     5821 gcgccggcgg ggccgacgtt cttctaccag ccgtcgatgc gcgcgccgtt cggcgcctgg
     5881 gcctgcgagg gacgcgccga cctcgtgcgc gtcgagcgcg acgcggcgac gggccgcgtg
     5941 gaggcgaccg tcatcgacat caaggcgagc cggcgcgaga ccgtgggctt ccgccttcag
     6001 gtcgccttct acgcgcgctt gctccgtggc gcgctggccg acgccggact cacgcccgcc
     6061 gcgattcacg gcgccatcgc cgcgcgcgac agtgagttga cagacgacgg cggctgggag
     6121 accttcgacc ttgcgctcta cagtgacgag gtcgagcgtc tggtggccgc cgccgactcg
     6181 gacgtggcgc gcgccgccgc cgccggcccc cacggcgcac gctatcacct gcgcgcggcg
     6241 tgcgacggct gcccctacaa ccagctttgc ttcgccgaca cggccgagcg gcttgacctc
     6301 tcgctcgtgc ctcacctcac catctccgag aagcgcgcgc tccacgcggc gggtgtcggc
     6361 gacgcgcggg agctggccgc gctcatggag tacggcgagg gcggcgcgat ggtcgtgagc
     6421 gcgggcgcgg aggcgcgctc ccgcgtcgtg gcttcgcgct ggccgctcgc gagccgtctg
     6481 cccgtgctcg tgcagcgcgc ccgcgccgcc gcccgccgct ttgaccgcgc gacggccgcg
     6541 cgtcgctacc tcgtcggttc ggactacggc tcgctccccg acccggcgca gtaccccgac
     6601 ctcgtgcgcg tcttcgttga cgcgcagcgc gactacctcg aagaccgcct ctatctcatc
     6661 ggcgcgcgcg tcaccggccc gcgcgcttcg tttgaagtga tcgagatgac ggacgcgccg
     6721 ccggacttgg actcagagcg cgctctgctc gtcgcgtggg tgcaaaactt gctgccggcg
     6781 gtcgcgcgcg cggccgagtc ggagcgcgcg ccgctgcacg tctacaccta cgcggcgcgc
     6841 ggcgagcgca cgttgctcga tgcgctcgcg cgccacttcg acgcactctg cgccatcccc
     6901 gccttctatg atctcctgac ctcgcgcccc gcgctctcgc aggggatgct ctcgctgctc
     6961 tccgaggagg tgcgcgcccg actcaatctt ccgcccgtct gtcagaacct ctacgaggtc
     7021 gcacgcgaac tcggcttcaa gtgggaagac ggccccgtag acttccgaaa aatcttccgc
     7081 gcgcgcgtct tcgacaaccg gcggacgttc gtgcgcgacg cggcggccgg tgtcttcacg
     7141 aaagacgcgg cgcgggccgt gaaggaagac gaagcgggag ctgttaacga tgacgaggag
     7201 gacgcgccgc cagagccgca ttccgtccgc gtcgaatcgg cggcgcgctt cggcgtggag
     7261 attccgttgg agtacgccta cgcggcgtgg gggcggctgc gggaaccgga ggcgtcggca
     7321 acacagccgg cgtcggggcg gcgttcgccg ttgcgcggtt tcgtgggcgt cagcgccgaa
     7381 gagatacgcg ctctcgaagg ggcgcggctg cgggccatgc agcacgtcga ggagagcttc
     7441 gcctacaaga accggcgcgt cgagaaggag ccgctcgatt tgtcgggact cgaccgcgtt
     7501 gacgtggaac ccgaagaggt tccgctccgc cgctcgctcg aagacttcct gctgctcgaa
     7561 caccacgcgc gctttcagga cttgctgctc cacctcgcgc tcccgccgcg ccagcgcgcc
     7621 gagaccgggc gcacgctcat gctccgctgc gagcgttatc tgaaagacga agacggcgag
     7681 cgcgcgcact ttcgcttcgc ggacgcggac acgaacgacg cggacggaac tgaagcggac
     7741 ggagttgtcg cgggcgacgc ggacgcagac ccgccgccgc agcagcaaca acagacgccg
     7801 ctccgcgtgc gcgcgggcga ctgggtggtc gtcaacccat cgcgcgacgg cgaggggcgc
     7861 gcgcttccgg cgtggagatt agtccgcggc cgtctcgcgg tcgtcgagga gctgagcgcg
     7921 tcggaagtcg ccttgcgcct gctgccgatg agcttcaaga actctccgtt tcgctacccg
     7981 catgtgcttt tcaagccgga gccgggcgtc gtctacaccg ccgacgagat ggccgacgat
     8041 ctcaactcgg acaagttcct cgaggcctgt cgcaacgccg caaccaatca cctctaccgc
     8101 tggctgagcg acatgggcga aggcaaacga gcgcgggccg tccgcccgcg gcgtctgcga
     8161 gacgcggagg cgatggcctc cctcgcgtcc gccgcacagt cgccgcacgg gctgaccggc
     8221 gcgcagcgcg agatcgtcgg cggtcatttg tgtgagcgcg tgctcgtcgt gcaggggccg
     8281 cccggcacgg gcaagagcca cacgctcggc ttcgccatcc tcgcgcgcgc gctggcgcaa
     8341 acgacgccgg cgcgcccctt ccgcgtcgcc gtgctcgcca agactcacgc ggccgtcgcc
     8401 gtcgccctcg aatcaatcgc gaagcgccgg cgcgagttga ccgaagcgat taagggcgac
     8461 gccgccacca ccgcacaccc ggccgccccg ctgcttgcgc cgctcgcgcg ccgtcggctc
     8521 gcgaagatgt gtaatgacgc gggcgagacg gtgcccgcgg gcgtcgagcg cgtgctgccc
     8581 gacgggggcg acgagtcaac agccgccgag cagtggcagt cgttgctcac ggaagaattg
     8641 ctcgtcgtcg gtgggacgcc gggcggactc tatcgtctgg tgaagctggg ggcgtcgaag
     8701 gggcgcgcgg tggattgggc ggcggagcat tttgatctgg tcgtcgtgga cgaggcgtcg
     8761 cagatggggc tggccgaggc tttgacggc
//
LOCUS       DASVES010000011         7895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1149925, whole genome shotgun sequence.
ACCESSION   DASVES010000011 DASVES010000000
VERSION     DASVES010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149925"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2433)
                     /locus_tag="VF240_00500"
     CDS             complement(<1..2433)
                     /locus_tag="VF240_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEX7173748.1"
                     /translation="MNKSLRSLALRLRASLPLNTRLAALCLLALLVCSAGLSSAVSGQ
                     DKTALRQEVDRALRSYDVLSLDTREVLDRVRQTQTLSLPTSAGTFDLVLTPHDILAPN
                     YRATATGDGGVVRELARPAVSTYKGTVRGMKGAQARLTIDGETVEGMIITPAQNYFIE
                     PASKFSPSAGKGDFVFYAESDVVEEKFGDCGVTMAEEVAAQAQRMEAGKGGAAFAKGG
                     DDQELFGPSYQVELATEADFEYFQAFGSAFAANQNIITIINQVNGIYETQIGLRFFIT
                     HQHVWETANDPYTTDAPGGLLDQFEAHWNNNFGNIARDLAHLWTGKDLGASTTTTDDD
                     STIGIASQPGLNCANGSGAYGLSQNITNGSNQRRSLLTAHEIGHNINAAHTNGVTECD
                     NTIMSPSLGTITQPSFCQLSRDQITDHSIQNISCLVSLVAPGCSYALSSSVQSFPAAG
                     GGGSFNVSATGGCNWGVAEGANWITVTAGDSGSGSGAVSYTVAPNTGGPRAARINVAG
                     QILTVTQQASAACGSTPVATGQTLNGTLGSPDCRSGQTGRVNAFIDIYTFGASAGQQA
                     RIVMNAPTPPPAGLDTYLYLYGPDGALIAENDDIVLGEQTNSRIPDNATGFLTLPATG
                     IYTIVATSYDNDDTGAYTLALTGNAQPPVASTVQFSASSATVTEGTDRRVDITVTRAG
                     DSSQAASVDYATSNVTASDRSDYLAAYGTLRWAAGDGSPKTFSVFIVDDVYGNTTTNG
                     QSDSAVETFNVTLSNPASATLGATAQMQVSIANNETADGANPVKAATFSPAFFVRQHY
                     LDFFNREPDASGL"
     gene            complement(2714..5353)
                     /gene="lptD"
                     /locus_tag="VF240_00505"
     CDS             complement(2714..5353)
                     /gene="lptD"
                     /locus_tag="VF240_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX7173749.1"
                     /translation="MVFPFGSSLPRSRPAVKLLLGVCALVMCLPLAAGPSVRAQQTNP
                     VDRQVNNPMTDTPNINPLSQTQPLVRPRRPQTNGAAAPAATPGQATDRLDVDSERQTV
                     TGPEDARVVVNEGNVDARIGIYRLQADKVTVYEAKNLVVAEGSVVFDQGEFQRITGSR
                     AEWNYATKTGFFLNSTGFTNQTQDGTLMYFTADRVDKISGDKIVVRSAVITACDEENP
                     KWSFTTKRAVITLNDRVRTKNPIFRVKGVPLLYLPYASISLKPKDRASGFLTPTFSGS
                     GKKGFRITNGYYQTLGRSADVTLRNDIFTGRGLGFGADLRTRANSRSYLDAGFYLIKD
                     RVFGAKADADNPDQGGSSFYMDGVHYFPNGFMAVADVNITSSLAFRAIFSDSIQQAIS
                     PEERSQVFVTKSFDDYSLNFLARTQVISIPSARIRLRNLPSISVDKRPSPLSFFKKWP
                     VYFSFESSMEGMSRKETIEDLNGFLAEGAQNAVVSPSIVQRLDFSPKVSVPLEFDGWT
                     ATASGRLRGTFYSNSLEAATRLVTARNLTRAYGEFEFDLRPPALARNFQRSDGSLRFR
                     HTIEPYFVYRKISGVGDFERIIRFDYMDAVADTNEIEFGITNRFFQRRSTETVTGRVS
                     AAARALGERDEDEETSRPLEPQPYEALSITVRQKYFFDPTFGGALIPGRRNQFYPINT
                     FSGFTYGGVPRRFSPLYVEARLRPYRNLESRLRSLWADVRANFDVLGEGGGLRDFAIT
                     SGFERRVPHLRLVRAFQTYYYTRAVTLAPSLQQYNDPRGNEPGTLQGSQWSPTAFLGD
                     PESGLYGGVSFFFDFQNRPGQGSSSLVSSTATVGYGWDCCAVTAQLFTFNVGLRQEKR
                     TVFSFRLNGIGTFGTEQIGQQFR"
     gene            5613..5969
                     /locus_tag="VF240_00510"
     CDS             5613..5969
                     /locus_tag="VF240_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173750.1"
                     /translation="MKLGLLNILVALFLGYGAVEELIVRGVRGGETQPFVIGLIGIVV
                     SVLLVVSGIALWQRRAGARRLAIVAAIASMLFHVYAALPPHRNVGILVLIVGAGYGLL
                     LLGVGLSSRGAKTQAA"
     gene            6052..7125
                     /locus_tag="VF240_00515"
     CDS             6052..7125
                     /locus_tag="VF240_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7173751.1"
                     /translation="MKIFRRVFSVATAAALLCLSQSADAGQQAAGASTPTAPGRMVDV
                     GGYRLHLNCVGRSGGPVVVLLAGGGDFSFDWSLVQPAVSRFARVCSYDRAGLAWSDLG
                     PVPRTMRQEAHELRALLERAGVRGPYVLVGHSFGGLIARVFAERYASDVAGVVLVDAT
                     SEDTTLMFGGKLVRVREGAKGKAVPPPRTLQDSPPLPPADADLKQAELNRQVFGPPKI
                     NPPFDKLPKQAQALRLWALSNPKLSAASEDFWADELQAMYVATGKTKHQLGDMPLVVV
                     IPHTKGDDRPPDGVSAADWARLRSEKRQQKLAFADLSRGGKVVFAEGSGHHVQLDAPE
                     VVVGAIRETVAAARRRTRPGRRK"
     gene            7293..7778
                     /locus_tag="VF240_00520"
     CDS             7293..7778
                     /locus_tag="VF240_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173752.1"
                     /translation="MENAVETSSFDRAHAGVRGNAWLRRFTVLTRVLLALGFLPSGIT
                     KLLGNRFTILGIDNPVGFFFEAFYRSGFYWNFIGACQLAAAALLIIPRTSTLGALVYF
                     PIILNIFVITVSMGFAGTPFITGLMLLANLYLLCWDYDRLKCLFAGRPPRKQQQQPAH
                     A"
BASE COUNT         1312 a         2418 c         2775 g         1390 t
ORIGIN      
        1 gaggcccgag gcgtccggct cgcggttgaa gaagtcgagg tagtgctggc ggacgaagaa
       61 ggcggggctg aaggtggccg ccttgacggg gttcgcgccg tcggccgtct cgttgttcgc
      121 gatgctcacc tgcatctggg ccgtcgcgcc gagggtggcg ctggcggggt tcgagagcgt
      181 gacgttgaag gtctccacgg cgctgtcgct ctggccgttc gtcgtggtgt tgccgtagac
      241 gtcgtccacg atgaagacgc tgaaggtctt cggggaaccg tcgcccgcgg cccaacgcag
      301 cgtgccgtag gcggcaaggt agtcggagcg gtcggacgcc gtgacgttgc tcgtggcgta
      361 atcgaccgac gccgcctgcg acgagtcgcc cgcgcgcgtg acggtgatgt caaccctcct
      421 gtccgtcccc tctgtgaccg tcgctgagct ggcgctgaac tgcaccgtcg aagccaccgg
      481 cggctgtgcg ttgccggtca gcgcgagcgt gtaggcgccg gtgtcgtcgt tgtcgtaaga
      541 ggtggccaca atcgtgtaga tgccggtggc gggcagggtc agaaagcctg tggcgttgtc
      601 aggtatgcgc gagttggtct gctctcccag aacgatgtcg tcgttttcgg cgatgagcgc
      661 cccgtccggc ccgtacaggt aaagataggt gtccagacca gccggaggcg gggtcggcgc
      721 gttcatcaca atccgcgcct gctgacccgc cgacgcgccg aacgtgtaga tgtcaatgaa
      781 cgcattgacc cggccggtct gtcccgaacg gcaatcgggg ctgcccagcg tgccgttcaa
      841 cgtctgcccg gtggcgacgg gcgtggagcc gcaagcggcc gacgcctgct gggtgacggt
      901 aagaatctga ccggcgacgt tgatgcgcgc cgcacgcggg ccgccggtgt tgggggcgac
      961 cgtgtagctg accgcgccac taccgctccc ggagtcgccc gcggtgacag taatccagtt
     1021 ggccccttcg gccacccccc agttgcaacc gcccgtggcg ctgacgttaa aactcccgcc
     1081 gccgcccgcc gccgggaacg attgcacgga ggaggagagg gcgtaggagc agccgggggc
     1141 gacaaggctg accagacagg agatgttctg tatggagtgg tcggtgattt gatcccgcga
     1201 caactggcag aaactcggct gggtgatggt gccgagcgag ggactcataa tggtgttgtc
     1261 gcattccgtc accccattcg tgtgggcggc gttgatgttg tggccgatct cgtgggcggt
     1321 gagcagcgac cggcgctggt tactcccgtt ggtgatgttt tgagataggc cataagcacc
     1381 gctgccgttg gcgcaattga gtccgggctg gctggcgatg ccgatggtgc tgtcgtcgtc
     1441 cgtggtggtg gtgctggcgc ccaaatcctt ccccgtccag aggtgtgcaa gatcgcgcgc
     1501 tatgttgccg aagttgttgt tccagtgagc ctcgaactga tcgagaaggc cgccgggggc
     1561 gtccgtcgtg tacgggtcgt tcgccgtttc ccagacgtgc tgatgggtga taaagaatct
     1621 caggccgatc tgcgtctcgt agatgccgtt gacctgattg atgatggtga tgatgttttg
     1681 attcgcggca aacgcgctcc cgaacgcctg aaagtactca aagtcggcct cggtcgcgag
     1741 ttcgacttga tagctcgggc cgaaaagctc ctgatcgtct cctccctttg cgaaggccgc
     1801 gccgcccttg cccgcttcca tcctctgcgc ctgcgccgcg acctcctcgg ccatcgtcac
     1861 gccgcaatcg ccgaacttct cctcgacgac gtccgactcg gcataaaaaa caaaatcccc
     1921 tttgcccgcc gagggcgaga attttgaggc cggctcgatg aagtagttct gagcgggcgt
     1981 gataatcatc ccttcgaccg tctcgccgtc gatagtcagg cgcgcctgcg cgcccttcat
     2041 gccgcgcacc gtccccttgt aggtgctgac ggccgggcgc gcgagttcgc gcaccacgcc
     2101 gccgtcgccc gtggccgtcg cgcggtagtt cggggcgagg atgtcgtgag gcgtcagcac
     2161 gaggtcgaac gtgcccgccg aggtcggcag cgaaagcgtt tgcgtctggc gcacacggtc
     2221 gagcacttcg cgcgtatcca gactcaacac gtcgtaactt cggagcgccc gatccacctc
     2281 ttgccggaga gccgtcttat cctgacccga gacggccgaa gaaagacctg cggagcagac
     2341 gagtaaggcc agcaggcaca gggcggcgag ccgcgtgttg aggggcagcg acgcgcgcag
     2401 gcgtagcgcg agggaacgaa gacttttatt caacaactta aactcccaga atgtcgaaga
     2461 ggattagagg acgtacacgc tcgcgacgca gacgcccgtc gggatgagga caacaacaac
     2521 aagggcacga ggccgacgac cggacgcctg cggagaagtt gtcatgggga gtggaaaacc
     2581 gcagccgagc gacccggtaa acatccaact tcagatgcac tgccctccgg gccggatgca
     2641 cttaaaaagt aacatttttc agggagatgc cgaaaccccc gcgagtcgtc ggggcgccac
     2701 ggggcgggga gtttcagcgg aactgctggc cgatctgctc ggtgccgaaa gtgccgatgc
     2761 cgttcaagcg gaagctgaag acggtgcgct tctcctgtcg cagaccgacg ttgaaggtga
     2821 agagctgggc ggtgacggcg cagcaatccc agccgtagcc gacggtggcg gtggagctga
     2881 cgagtgagga gctgccctgg ccggggcggt tctggaagtc gaagaagaag gagacgccgc
     2941 cgtagaggcc gctctccggg tcgccgagga aggccgtggg gctccactgc gagccttgca
     3001 gtgtgcccgg ctcgttgccg cgcgggtcgt tgtactgctg gagcgagggc gcgagcgtga
     3061 cggcgcgcgt gtagtagtag gtctggaagg cgcgcacgag gcggaggtgc ggcacgcggc
     3121 gctcgaagcc ggaggtgatg gcgaagtcgc gcaggccgcc gccttcgccg agcacgtcga
     3181 agttggcgcg cacgtcggcc cacaggctac gcaggcgcga ctccagattg cggtagggcc
     3241 gcagacgcgc ctcgacgtag agcggcgaga agcggcgcgg gacgccgccg taggtgaagc
     3301 cggagaaggt gttgatgggg tagaactggt tgcgccgccc cggaatgagc gcgccgccga
     3361 acgtggggtc gaagaaatac ttctggcgga cggtgatgga gagcgcttcg tagggctgcg
     3421 gctcaagggg ccgggaagtc tcctcgtcct cgtcgcgctc gccgagagcg cgcgccgccg
     3481 ccgagacgcg ccccgtcacg gtctcggtcg agcgtcgctg gaagaagcgg ttggtgatgc
     3541 cgaactcgat ctcgttggtg tcggcgacgg cgtccatgta gtcgaagcgg atgatccgct
     3601 cgaagtcgcc gacgccggag attttgcgat agacgaagta aggctcgatg gtgtggcgga
     3661 agcgaagcga accgtcggag cgctggaagt tgcgcgcaag cgccggcggt cggaggtcga
     3721 actcgaactc gccgtaagcg cgcgtgaggt tgcgggcggt gactaagcgc gtggccgctt
     3781 cgagcgagtt cgagtagaag gttccgcgca ggcggcccga ggcggtggcc gtccagccgt
     3841 cgaactccag ggggacggag accttggggc tgaagtcgag gcgctggacg atggagggcg
     3901 agacgacggc gttctgcgcg ccttcggcga ggaagccgtt caagtcttca atcgtctcct
     3961 tgcggctcat gccctccatg ctcgactcga acgagaaata gaccggccac ttcttgaaga
     4021 aggagagggg gcttgggcgc ttgtccacgg agatgctcgg caggttacgc aggcggatgc
     4081 gcgccgaggg gatggagatg acctgcgtgc gcgccagaaa gttgaggctg tagtcgtcga
     4141 aactcttggt gacgaagacc tgcgagcgct cctcgggcga gatggcctgc tggatcgagt
     4201 cggagaagat ggcgcggaag gcgaggctcg aagtgatgtt gacatcggcg acggccatga
     4261 agccgttggg gaaatagtgg acgccgtcca tgtagaagct cgacccgccc tgatcggggt
     4321 tgtcggcgtc ggccttggcg ccgaagacgc gatccttgat gaggtagaag ccggcgtcga
     4381 gataggagcg cgagttggcg cgggtgcgca ggtcggcgcc gaagcccaag cctcggcccg
     4441 tgaagatgtc gttgcgcagc gtcacgtcgg ccgagcgccc gagcgtctgg tagtagccgt
     4501 tcgtgatgcg gaagcccttc ttgccggagc cggagaaggt gggggtgaga aagcccgagg
     4561 cgcgatcctt cggcttgagc gagatggagg cgtagggcag gtagagcagc ggcacgccct
     4621 tgacgcggaa gatggggttc ttcgtgcgca cgcggtcgtt taaggtgatg acggcgcgct
     4681 tggtcgtaaa actccacttc gggttctctt cgtcgcaggc ggtgatgacg gcgctgcgga
     4741 cgacgatttt gtcgccgctg attttgtcaa cgcggtcggc ggtgaagtac atcagcgtgc
     4801 cgtcctgcgt ttgattggtg aagccggtgg agttgaggaa gaagccggtc ttggtggcgt
     4861 agttccactc ggcgcgcgac cccgtgatgc gctggaactc gccctgatcg aagacgacgc
     4921 tgccctcggc gacgacgagg ttcttcgcct cgtagaccgt caccttgtcg gcctgaaggc
     4981 gatagatgcc gatgcgcgca tcgacgttcc cctcgttgac gacgacgcgc gcgtcctccg
     5041 ggccggtgac ggtctgccgc tcggagtcaa cgtcgagccg gtcggtcgcc tgccccggcg
     5101 tggcggccgg agcggcggcg ccgttcgtct gcgggcggcg tgggcgcaca aggggctgcg
     5161 tctgcgagag cgggttgatg ttcggcgtgt ccgtcatcgg gttgttcacc tgacggtcca
     5221 cggggttggt ctgctgcgcg cgcacgctcg gcccggccgc gagcggcagg cacatcacga
     5281 gcgcgcagac gccgaggaga agcttgactg ccgggcgtga gcgtgggagt gaactgccga
     5341 aggggaacac catgcgtaag ggtctttaag tggtggcggt cgaatctccg aaggtgaaac
     5401 ttccgcgcgc gcggcctcgt ctcggggagg ggccggcgcc agcgcgccgc gaagtttcaa
     5461 aaacgcggcg atgctaacac gcgcccccga cgtgccacaa ggcaacagta aacggccgca
     5521 tcacccgaat gattttgcgg gccggaaaca aacggggccg ggcggtgtct aacgaactgc
     5581 tttccaaaaa actgtccgta aaggggtttg gcatgaaact cggtctgctg aacattctgg
     5641 tcgcgctgtt tctcggttac ggcgcggtgg aggagttaat tgtgcgcggt gtgcgcggcg
     5701 gagagacgca gcccttcgtc atcggtctca tcggcatcgt cgtcagcgtt ctgctcgtag
     5761 tctcgggcat cgccctgtgg cagcgacggg ccggtgcgcg ccgcctcgcc atcgtcgcgg
     5821 ccatcgcgtc catgttgttc cacgtctacg cggcgctgcc gccgcaccgc aacgtgggga
     5881 ttctggtgct catcgtcggg gcgggctacg ggctgttgct gctgggcgtc ggtctgagtt
     5941 cgaggggcgc gaagacgcag gcggcctaga ggcggcgcac actgtcccgc gcctcatcgc
     6001 cgacgccgct ttcaaaatgc aaccacaccc gacttgcggc gtgtcggcgt tatgaaaatt
     6061 tttcgacgtg ttttctcagt cgcgaccgcc gccgcgctgc tctgcctttc gcagtcggcc
     6121 gacgccgggc aacaggcggc cggcgcgagc accccgactg ctccgggccg aatggttgat
     6181 gtgggcggct accgtctgca ccttaactgt gtgggcagga gcggcggccc cgtcgtggtg
     6241 ctgctcgccg gcggcggcga cttctccttc gactggagtc tggtgcagcc cgccgtctcg
     6301 cgtttcgcgc gcgtctgttc ttacgaccgc gccggcctcg cctggagcga cctcggcccc
     6361 gtgccgcgca cgatgcggca ggaggcgcac gagctgcgcg cgctgcttga aagggcgggg
     6421 gtgcgtgggc cttacgtgct cgtcggccac tcgttcggcg gcctcatcgc gcgcgtcttc
     6481 gccgaacgtt atgcgagcga cgtggccggc gtggttctgg tagacgccac cagcgaggac
     6541 acgacgctga tgttcggagg caagctcgtg cgcgtgcgcg agggcgcgaa ggggaaggcc
     6601 gtcccgccgc cgcgcacgtt gcaggacagc ccgccgcttc cgcccgccga cgccgacctc
     6661 aagcaggccg aactcaaccg tcaggtgttc ggcccgccga agatcaatcc accgttcgac
     6721 aaattgccga agcaggcaca ggcgctgcgt ctgtgggcgc tctccaaccc gaagctctcg
     6781 gccgcctccg aagatttctg ggcagacgag ttgcaggcga tgtacgtcgc caccggaaag
     6841 acgaagcatc aactcggaga catgccgctc gtcgtcgtca ttcctcacac gaagggcgac
     6901 gacaggccgc ccgacggcgt ctcggcggcg gactgggcga gactccgaag cgagaagcga
     6961 cagcagaagc tggccttcgc cgacctctcg cgcggcggca aggtggtctt cgcggaaggc
     7021 agcgggcatc acgtccagct cgacgcgccc gaggtggtcg tcggcgccat ccgcgagacg
     7081 gtcgccgcgg cgcgtcgccg cacgaggccc ggccgaagga agtgaagacg gcaatccagc
     7141 aaacgcgcgc gcccggccac acgtcgccgg ccggcgacga gcgcgcacgc ggctgatgaa
     7201 acccgcgcac cgcacgcgtc gtacacacgg gcgttcggtc aacgccggta gcgaggcgtt
     7261 aacgggcgcg cgattttttg tgagtcggga ggatggaaaa cgccgtggag acttcatcgt
     7321 ttgaccgcgc gcacgcgggc gtgaggggca acgcgtggct gcgccgtttc accgtcctca
     7381 cgcgcgtgct cctcgccctc ggctttctgc cctcgggaat cacgaagctt ttgggcaacc
     7441 gcttcaccat cctcggcatt gataatcccg tcggcttctt cttcgaggcg ttctaccgtt
     7501 ccggcttcta ctggaacttc atcggcgcct gccagctcgc ggcggccgcg ctcctcatca
     7561 tcccccgcac ctccaccctc ggcgcgctcg tctacttccc catcatcctc aacatcttcg
     7621 tcatcaccgt ctccatgggc ttcgccggca cccccttcat caccggcctc atgctcctcg
     7681 ccaacctcta cctgctctgc tgggactacg accgcctcaa gtgcctcttc gccgggcggc
     7741 cgccccggaa gcagcaacag caacccgcac acgcgtgagc gacaaccctt gaagtacagt
     7801 accgcgagcg gaagcctcgc gggtctcgcg tgtgcggggt gggcctgtca tcgtaggccg
     7861 cacccgctcg ctaccgctcg cggtactgtt cccac
//
LOCUS       DASVES010000012         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1733930, whole genome shotgun sequence.
ACCESSION   DASVES010000012 DASVES010000000
VERSION     DASVES010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733930"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1588)
                     /locus_tag="VF240_00525"
     CDS             complement(221..1588)
                     /locus_tag="VF240_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEX7173753.1"
                     /translation="MEYTQAEIFQTVRMTEMEHLDVRTVTLGLSLRDCAREHIEDTAR
                     LVREKIERTAARLVSTVDEIGDEIGLRIANKRISITPAAIIAGGARSTADYVTLARAI
                     DEAAATVGVDFIGGYSALVHKGATRAEEMFLDSIPEALSTTKRLCSSVNVATTRAGIN
                     MDAVARVGRIIKDAAERTRDAGAIACAKFVCFANAVEDNPFMAGAFHGIGEPEAVINV
                     GVSGPGVVNHALRHAPQEMPLDELAELIKKLSFKLARAGELVGREAARRLGIQFGIID
                     LSLAPTPVPGDSVAEILEAFGFERAGAPGTTAALALLTDAVKKGGAMASSSVGGMSGA
                     FIPVSEDAAMIAAARLGALTLEKLEAWTSVCSVGIDMVAVPGTTSAETLSAIIADEMA
                     IGVVNNKTTAVRIIPVPGKDVGDMVEYGGLLGSAPIMSVNRFDSSVFIRRGGRIPAPI
                     HSLRN"
     gene            complement(1614..2261)
                     /locus_tag="VF240_00530"
     CDS             complement(1614..2261)
                     /locus_tag="VF240_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7173754.1"
                     /translation="MSCGAAVRAGARFCPQCGKLMEAEAPAEVARADEGDAPAGAPPA
                     ADVAPAAADEIRLEESWRSWEESVGARTDAAAKVEAPAREQPREEVSGESRGGVGETP
                     VPLTRPLQRPQSTLPAPSVVQREGHDDGRGDEGAAASASAGVGGPLARPRAGEKGSRA
                     AAVKESLRPRVERVRDASMGVLEDAAEDSGLRFVLIAIVLFAVFLVFLFINNLVR"
     gene            complement(2415..2876)
                     /locus_tag="VF240_00535"
     CDS             complement(2415..2876)
                     /locus_tag="VF240_00535"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7173755.1"
                     /translation="MANEELIYRITRAVYGRLGKEADERLVEGLVTDIYQAVEPLAGA
                     NGGGAGGGLEQGATRADGSADRLIVSVFGVDHPGIVAAVSQILADADCSIIDINQTVV
                     SGKFAMVIVADTTRARQSASELKERFRHEGERLGVRIYAQREDLFNAMHRV"
     gene            complement(2965..3297)
                     /locus_tag="VF240_00540"
     CDS             complement(2965..3297)
                     /locus_tag="VF240_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX7173756.1"
                     /translation="MFLGKVVGTVWSTKKTPDLEGVRFLIVHPIDLDKEPTKNIVVVA
                     DRLGAGPGEVVLCAYGKAARSAIGNQEMSIEAAVVGIIDRMDLTESESDELIEAEREL
                     RAPDNGRR"
     gene            complement(3381..>3993)
                     /locus_tag="VF240_00545"
     CDS             complement(3381..>3993)
                     /locus_tag="VF240_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173757.1"
                     /translation="KRPARSSSASTSAQPQPAMAQTPARPSVAASPAGIQAGAGGVSR
                     EEIAKIVDRFLTGREGAQGGQTSGGGAATMRPTDSPAPVQTGIDIEPQRVSPSGGAAP
                     QPSAAPTAQTDGGNGSQQGAPSQYNAPPPPAQRPAASSPPAENGGKQPAASTNGKRAV
                     DFVCEDDVRRAMQKGEKIYIGARTIITPAARDMGEGAEVFAKA"
BASE COUNT          621 a         1338 c         1314 g          720 t
ORIGIN      
        1 caacagattt tcgtcagacg tgttcatccg ccgcggcggt cgcatcccgg cgccgattca
       61 ctcgttgagg aattgaaaca tggatggaca ggatgggcag gacgagaaga agtgatgagt
      121 gatgagtgat gagtgatgag aaagagcaga aaccgctttc ttccttctca tcactcatca
      181 ctcatcactc atcacctctt gtatcctgtc catccatgtt tcaattcctc aacgagtgaa
      241 tcggcgccgg gatgcgaccg ccgcggcgga taaagactga gctgtcgaag cggttgacgc
      301 tcatgatggg ggcgctgccg agcaagccgc cgtactcgac catgtcgccc acgtctttgc
      361 cggggacggg gatgatgcgc acggccgtcg tcttgttgtt gacgacgccg atggccatct
      421 cgtcggcgat gatggcggag agggtctcgg ccgaggtcgt gccgggcacc gccaccatgt
      481 cgatgccgac tgagcagacg ctcgtccacg cctccaactt ctccagcgtg agcgcgccga
      541 gcctggccgc cgcgatcatc gccgcgtcct ccgagacggg gatgaacgcc ccgctcatcc
      601 cgcccaccga agacgaggcc atcgcgccgc ccttcttgac ggcgtcggtc aggagcgcga
      661 gcgcggccgt cgtccccggc gcgcccgcgc gctcgaagcc gaacgcttcg agtatctccg
      721 ccaccgagtc gccgggcacg ggcgtcgggg cgagcgacaa atcgatgatg ccgaactgga
      781 ttccgagccg gcgcgcggcc tcgcgtccga caagctcgcc ggcgcgcgcc agcttgaagg
      841 agagcttctt gatgagttcg gccagctcgt cgagcggcat ctcctgcggc gcgtggcgca
      901 gcgcgtggtt gacgacgccc ggccccgaga cgccgacgtt aatcaccgcc tccggctcgc
      961 cgatgccgtg aaacgcgccg gccatgaacg ggttgtcttc gacggcgttg gcgaaacaga
     1021 cgaacttcgc gcaggcgatg gctcccgcgt cgcgcgtgcg ctcggccgcg tccttgatga
     1081 tgcgcccgac tcgggcgacg gcgtccatgt tgatgccggc gcgcgtcgtc gccacgttca
     1141 ccgacgagca caggcgcttc gtcgtcgaga gcgcttcggg gatggagtcg aggaacatct
     1201 cctcggcgcg cgtcgctccc ttgtggacga gcgccgagta gccgccgatg aaatcgacgc
     1261 cgacggttgc ggcggcctcg tcgatggcgc gcgcgagtgt gacgtagtcg gccgtgctgc
     1321 gcgcgccgcc ggcaatgatg gcggcgggcg taatcgagat gcgcttgttg gcgatgcgca
     1381 gtccgatctc gtcgccgatt tcatcgacgg tggagactaa gcgcgcggcc gtgcgctcga
     1441 tcttctcgcg cacgaggcgg gccgtgtcct cgatgtgctc gcgcgcgcag tcgcgcagcg
     1501 agaggccgag cgtgacggta cgcacgtcca agtgctccat ctcggtcatc cgcacggtct
     1561 gaaaaatttc cgcctgggtg tattccataa gaggatagcc gccgcggccg ccgtcacctg
     1621 acgaggttgt tgatgaagag gaagacgagg aagacggcga agagcactat ggcgatgagc
     1681 acgaagcgca gccccgagtc ttcggcggcg tcctccagca cgcccatcga ggcgtcgcgc
     1741 acccgctcga cgcgcggacg cagagactcc ttgacggccg ccgcgcggct gcccttctcg
     1801 cccgctcgcg gtcgtgcgag tggcccgccg acgccggccg acgccgacgc tgccgcgccc
     1861 tcgtcccctc gtccgtcgtc gtgtccctcg cgctgcacga cgctcggtgc gggcagtgtt
     1921 gactggggcc gctgtagcgg ccgtgtcagc ggcacaggcg tctccccgac gccgccgcgc
     1981 gactcaccgc tgacctcttc gcgcggctgc tcccgcgcgg gcgcctcgac tttcgccgcc
     2041 gcgtcagtgc gcgcgccgac gctctcctcc caactcctcc aactctcttc gagccgaatc
     2101 tcatcggccg ccgcgggcgc tacgtctgcg gccggcggcg ccccggcggg cgcgtcccct
     2161 tcgtccgctc gcgcgacttc cgccggcgct tccgcctcca tcaacttccc gcactgcgga
     2221 cagaagcgcg cgcccgcgcg caccgccgcg ccgcagctca agcatcgacg tgagatttcg
     2281 ggaagcattg gaaagccgtg ataagtgatg agtgatgagt gataagaaag agaagaagct
     2341 tttagctgtt ggctgttagc taatcgctaa gtgctaacag cctcttatca cttatcactc
     2401 atcacttatc accctcaaac cctgtgcatc gcgttgaaga ggtcttcgcg ctgggcgtag
     2461 atgcggacgc ccagacgctc gccctcgtgg cggaagcgct ccttcagctc ggaggccgac
     2521 tgccgggcgc gcgtggtgtc ggcgacgatg accatcgcga acttacccga gacgaccgtc
     2581 tggttgatgt cgatgatgct gcaatcggcg tcggccagaa tctgcgagac ggcggcgacg
     2641 atgccggggt gatcgacgcc gaagaccgag acgatgaggc ggtcggccga gccatcggcg
     2701 cgcgtcgcgc cctgctccag ccccccgcct gcgccgccgc cgttcgcgcc cgccaacggc
     2761 tcgaccgcct ggtagatgtc cgtcaccagc ccctcgacca agcgttcgtc ggcctccttg
     2821 ccaagcctgc cgtagacggc ccgcgtgatg cggtagatga gttcctcgtt agccatcgct
     2881 gaagaagtaa aaaggtaaaa gtaaaaaggc aaaaggggaa gtgcgtccgg gtctccttaa
     2941 cttttgccct ttgccttttt acttttacct tcgcccgttg tcgggcgcgc gcagctcgcg
     3001 ctccgcctcg atgagttcgt cggactcgga ttcggttaag tccatccggt cgatgatgcc
     3061 gacgacggcg gcctcaatcg acatctcctg gttgccgatg gccgagcgcg ccgccttccc
     3121 gtaggcgcac aacacgacct cgcccggccc cgcgccgagg cggtcggcga cgaccacgat
     3181 atttttggtc ggctccttgt ccagatcaat cgggtggacg atcaaaaaac ggacgccctc
     3241 aaggtcgggc gtcttcttcg ttgaccacac ggtgccgact actttgccta ggaacataag
     3301 aattcaggag tcaggagtca ggagtcagaa gtcaggatga agacgttttt attctgactc
     3361 ctgactcctg actcctgact tcatgccttc gcgaacacct ccgcgccttc gcccatgtcg
     3421 cgggcggcgg gggtgatgat ggtgcgtgcg ccgatgtaaa tcttttcgcc cttctgcatg
     3481 gcgcggcgca cgtcgtcttc gcagacgaaa tcgacggcgc gcttgccgtt cgtcgaagcc
     3541 gccggctgtt tgccgccgtt ctcggcgggc ggtgacgacg cggcgggtct ctgcgcgggc
     3601 ggcgggggcg cgttgtattg cgacggcgcg ccctgttgcg agccgttgcc gccgtcggtc
     3661 tgcgcggtgg gcgcggccga gggctgcggg gcggccccgc cggatggcga gacgcgttgc
     3721 ggctcgatgt cgatgccggt ctggacgggc gcgggcgagt cggtgggccg catcgtcgcc
     3781 gcgccgcctc ctgaagtctg tcccccttgc gcgccctcgc gcccggtcag gaagcggtca
     3841 actatcttcg cgatctcctc gcggcttacg ccgcccgcgc cggcctgaat gccggcggga
     3901 gaagcagcga cggatggccg cgccggcgtc tgcgccatcg cgggctgcgg ctgcgcggaa
     3961 gtgctggcgc tgctgctgcg ggccgggcgc ttc
//
LOCUS       DASVES010000013        14045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1342728, whole genome shotgun sequence.
ACCESSION   DASVES010000013 DASVES010000000
VERSION     DASVES010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14045
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342728"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VF240_00550"
     CDS             <1..559
                     /locus_tag="VF240_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7173758.1"
                     /translation="ENVTMPLMPRVETSRLLLRPFTAEDLDDFSEILGKPGVMKYLGT
                     DCQPMSRAETETALRSMIGHWRRHGFGRWAVVSKEGGRLIGCAGLRSYQAVAELVYLL
                     DEPYWGRGLATEVARACLRFGFETQGFDRIVAFARLRNAASRRVLDKLGMSFDGETTV
                     FGIHVAQHSLRREEFRPDHSAYEIK"
     gene            complement(898..3408)
                     /locus_tag="VF240_00555"
     CDS             complement(898..3408)
                     /locus_tag="VF240_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7173759.1"
                     /translation="MFTIIALGAVAAAYSALRISVPDLGLPFLLFALITLGFGSRIVV
                     AIPRTRGHISVSDTFILLSLLLFGGEAGVLLAGADALFSSARISKYKLTVVFNTAVYI
                     CSTFVTLWALRLLFGPLPALTQQGYTSVYVMAICVMGFVQYAANSGLVAMGVALRGGQ
                     PVWRMWRDNFLWTSLTYFAGASAAGIIAKLIGSLGVYAFLATAPIVAVVYFTYTTYLK
                     NVESATTQAELAQRHVEELNRQMAEQARISRALKESEEHFRNAFDHAVGMALISPKGH
                     WLQVNGSLCAMLGYDETEMLAGSFRAVTHPEDLGESLIKMHQLLEGEVSSYQLETRYV
                     HKDGSAIWVLQSASLVRGADGRPRHLIFQIQNVSDRKRAEEQIHHAAFHDSLTGLPNR
                     TRLSDRLSLAVERAKRASSYRFAVLFVDLDRFKVVNDSLGHDLGDRLLIDLSRRLEDC
                     VRRVDTVARLGGDEFAILLDGINAPSDATDVAARIQEALARPFDLDGHEFFTTASVGI
                     AYSATGYDRHEDILRDADTAMYRAKANGKARYEVFNTGMHTRAVEALRLESELRHAIE
                     AVEVRPHFQPIVSLETGVVTGFEALARWHHPQRGLVSPADFIPLAEETGLIVPLGMSI
                     LRQACRQLSRWQGPDDDGRPALTLSVNISVKQFRQIDLVEEIRGILVSSGIEPERLRL
                     EITESALMEDAAGAVEILRQMKAIGVQLSIDDFGTGYSSLSYLHRFPIDILKIDRSFV
                     SRMNTDKESRGIVETIIILANKLGKEVVAEGVELEEHRAALASLSCRFAQGFLFSKPL
                     DAEAATAFLTAAGRSFGAYDSAGLWHEDFDQVGSVYAM"
     gene            complement(3778..4668)
                     /locus_tag="VF240_00560"
     CDS             complement(3778..4668)
                     /locus_tag="VF240_00560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7173760.1"
                     /translation="MERKAASDYPQELLDLFHEYQHGDISRRAFLAGAAKFAVGGLTV
                     AAIFEGLTPNYAWAQQAPPGDKRIKVGYEVVPSPAGNGSIKGYLARPAQGKKKLPVVL
                     VIHENRGLNPYIEDVARRLALAKFVAFAPDGLTSVGGYPGSDEKGAVAFREVDGMKMT
                     EDFVAAAKWLKARPDSTGKLGAVGFCFGGGMVNQLAVRLGADLNAGVAFYGRQAGVDD
                     VPRISAPLLFHYASNDERVNAGIAAYEAALKANKKVYTSHMYEGKQHGFHNDTTPRYD
                     ETAAKLAWTRTLEFFNKYLR"
     gene            5171..6136
                     /locus_tag="VF240_00565"
     CDS             5171..6136
                     /locus_tag="VF240_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7173761.1"
                     /translation="MLRRFVRCACFGLLLLSLPALPAFAQQGAGGPASPAMQEANALY
                     TAQKWEEAAKAYEMIARENPKSGVAWFRLGNSLHALKQYERAIEAFRHSVEIGNNSTA
                     MYNIACGYALMGRKEEAFEWLFRSLKAGFIPAGAGLASDADLASLTGDARFKEASALG
                     EKNARPCTVTPEHRQFDFWVGEWNVYNPQGTQVGTNTIENVVGGCIVMENWTGAGGFV
                     GKSMNFYDPATRKWRQTWVDGRGSVLDFAGEFKDAAMRYTGETAGANGARTLARLTFF
                     HLGPDRVRQLWEQSSDGGKTWSVVFDGMYLRKGTEGAAPGKSGAK"
     gene            complement(6305..6733)
                     /locus_tag="VF240_00570"
     CDS             complement(6305..6733)
                     /locus_tag="VF240_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173762.1"
                     /translation="MTNKYESMEGILRLYELRREETMRQARAWFVGFHPTSAQDIHNV
                     FRGDESAYYRMVTSYWDMACSFVNHGAIDEQMFNDTVFEHVAVFAKVQPFLEELRAAS
                     GVPQYLRHLEQVVTRMPDSEALVARMREVTKAMTAGRARE"
     gene            complement(6807..7535)
                     /locus_tag="VF240_00575"
     CDS             complement(6807..7535)
                     /locus_tag="VF240_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7173763.1"
                     /translation="MLKKILIPVLFVLFIALFPLTAGHAAEPDDRDDARQSIREGNEK
                     FARGRYEEAVESYRKALAGPVELSATARYNIGVCLYELGRTREAVAAYREAVAARRGN
                     YAKASYALGVALEDLEEWAGAEAAYREAVGASGGRHAEAHFRLGVMAARAGANERAGA
                     LFKRAVEISGDRFPTARNNLGVMLALAGRHREAECEFAAALAQGGDAYEDARRNLALC
                     RSLLLRQRTTDEVGRLTLTASPPR"
     gene            complement(7604..8542)
                     /locus_tag="VF240_00580"
     CDS             complement(7604..8542)
                     /locus_tag="VF240_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7173764.1"
                     /translation="MFEDECRLDDEQESPVVSKSSTRWQGIRLERYRLRRGQFPEHAH
                     EEHAAIVSLGGGCRGEVRTANGFRVGGRNRAGSLILVPARRPHTISLDGDAECVSLYL
                     DPLIVARAASEAGTGAGEIVERRAAYDPVVANVAQALLAESERGEALYGRLYAESLAN
                     VLAIHLLRNYAATTSAAAPARFSGGLSGPALRRVLAYMADNYGHDLSLTDLAGEAGIS
                     TFHFAREFKRSTGEAPHQYLIKLRVERAKGLLAEGRLPIVEVGLRTGFNSQSHFARLF
                     RRLTGTTPGSYRGASRARREYVRAPSSKNGQTPATT"
     gene            complement(8529..9233)
                     /locus_tag="VF240_00585"
     CDS             complement(8529..9233)
                     /locus_tag="VF240_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7173765.1"
                     /translation="MTTSATAKAVRAKGDRTRQAILRAAVDVASEEGLEGLTIGRLAT
                     EMGMSKSGLFAHFGSKEELQLATVEAAREVFIEAVIHPSFESTRGLARLRRLCEVWLD
                     YANGQVFRGGCFFFAASAEFDGRPGPVRDRVAAIMREWLTTLERTAREAQAAGELSRD
                     VTPAQLAFEINALELGANWAFQLYGDARVFAQTREAILQRLRGLSAADAPQATRPRPK
                     RSPRKTKAPEKKHVRG"
     gene            complement(9519..11192)
                     /locus_tag="VF240_00590"
     CDS             complement(9519..11192)
                     /locus_tag="VF240_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7173766.1"
                     /translation="MLKRRLAILLVFVCIYVPLPAGGQEPAAARTERINAEVFWKIRR
                     EGTDNSQIMRTIHFLTDLYGPRLTGSPSHKAAAEWAAKQMESWGMKNARLEPWDFKHP
                     GWQNERLSAHIVAPVKDALVCEALAWTPGTNGTVTGRAFQLITPERPTRERLDAYLNS
                     IRERVRGGIVLVGRHQTVPVSFNPPFKRRDEQDARNAFDPNTPPAPPFVFPSPTPTPT
                     PGPDQPLPPQRVAEAVEQFLVDNGALVRVNDAGREHGQIRAFNNRTFDVTKAVPTVVM
                     RNEDYGRISRLLADAAPVELEFNIVNRTYPEGRTSYNVVAEIPGDGERDEVVMLGGHL
                     DAWHAATGATDNAVGCAIMMEAARILKAIGAKPRRTIRVALWSGEEQGLLGSQAYIKE
                     HFGTAENPKPDYSKFNGYFNIDSGTGRVRGATVFGPPEAATILRQALLPFADLGVEGA
                     IATRSRNLGGSDHTSFNQSGLPGIGMGQDPIEYGTHTWHTNLDTYERIVEDDVKAAAV
                     VIAAAVYHLASREEPLPRFTKERMPAPPPTPSPSPTPTPSPAATPVTQL"
     gene            11440..11847
                     /locus_tag="VF240_00595"
     CDS             11440..11847
                     /locus_tag="VF240_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX7173767.1"
                     /translation="MRRVKSKQPLRRPTELELSILQVLWGHGPGTVREVLQRFNDGRG
                     SEAGYTTVLKMLQIMTEKGLVERDDARRPQVYRARLSQEQTQRQLVSDLLERAFGGSA
                     KQLVMQALATRKTSEADLTQIEKLLDKLEGGTE"
     gene            11844..13949
                     /locus_tag="VF240_00600"
     CDS             11844..13949
                     /locus_tag="VF240_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7173768.1"
                     /translation="MNNLEDVLQQTVFHAVGWALVHSLWQGAGVAALLAGLLALLRGR
                     HSRARYAAGCAALALMLLLPATTVWKMSGGSESAHAASDETGRRRENHARTGTLEATV
                     IAAGAVEDAPEGFAYESHDAGRHTSTGGRFEGVLPWLSLLWALGVAVFSARSLGGLIY
                     ARRLKSVGTRPAAREWQQRLAELSRQLRVKRAVRLLESTLVQVPTAVGWLRPVVLLPA
                     SALTGLTPRQLEVVLAHELAHIRRHDYLVNLLQTAVETLLFYHPAAWWVAGRVRAERE
                     HVCDDMAVSATGGDALGYARALTALERLRPHARPQLAMAIDGGSLRSRIMRLVGAPAA
                     SRRTSPLLAGVAFAAALITTVACTGAVLSQNKKPAGNSGNEPGAAKGEAANAAREKSN
                     AEVANLSPAMQKLVAGDSTEGEDTEVRRAALAALGKRNGSVVVMDPSTGRVYAVVNQE
                     WALRRGWLPASTMKVIVSMAGLGERTLDPAQKVKVSDRPERINLTDALAFSNNPYFFP
                     VSERVGSEAILAYARRLGLGEPTGINYEGETAGILPASARGTNAGRLGTHGEGVEVTP
                     VQLAALTSAIANGGTLYVPRVPRTAEEAARFEPKVRRSIDIPREILDRITPGLLATVE
                     RGTGVGAKLEALKVAGKTGSAGGKNHSHGIFYSYAPAERPRLVVVVVMEGPAENGRES
                     AGVAGAIYKALGSRLAGGE"
BASE COUNT         2342 a         4388 c         4900 g         2415 t
ORIGIN      
        1 cgaaaacgtg acaatgcccc tcatgccccg cgtcgagacc tcgcgcctgc tcctgcgccc
       61 gttcaccgcc gaagaccttg acgacttcag cgaaatcctc ggcaagcccg gcgtgatgaa
      121 gtacctcggc acggactgcc agccgatgtc gcgcgctgag actgagacgg cgctccgcag
      181 catgatcggc cattggcggc ggcacggctt cggccggtgg gcggtcgtca gcaaagaggg
      241 cgggaggttg atcggttgcg ccgggctgcg ctcgtatcag gcggtggccg aactcgtcta
      301 cctgctcgac gagccatact gggggcgggg gttggccacg gaggtcgcgc gggcctgtct
      361 gaggttcggg ttcgagacgc agggcttcga ccgcatcgtc gcgttcgccc ggttgcgtaa
      421 cgcggcctcg cgcagagtgc tcgacaagct cggcatgagt ttcgacgggg agacgactgt
      481 cttcggcatc cacgtcgcgc agcactccct gcggcgcgag gagtttcgcc ctgatcactc
      541 cgcatacgaa atcaagtaaa aagtaaaaag gcaaaaggca aaagtgaaga cgggcgggtc
      601 gcgagcgttc aacgagccgc ggagcggcgg aagaatgtag cccacggcgt cagccgtggg
      661 gacaaggtca attaaataac cggaagcccc ggaggggcga aagagtcttg agccttggca
      721 tcacgcccgc ccatattctt tcgcccctcc ggggcttcgt ttctctgtaa acgctgtgac
      781 cccggtctca cgaccggggc tacattcttc caccgcctcc ggcggctcgt cgtcgcctgc
      841 gactcggcct ctccaacttt tgccttttgc ctttttacct ttgccttacg ccatgtctca
      901 catggcgtag acgctgccca cctgatcgaa atcttcgtgc cagaggccgg cggagtcgta
      961 ggcgccgaag gatcgccccg ccgcggtgag gaaggccgtg gccgcttcgg cgtcgagcgg
     1021 cttcgagaag aggaacccct gtgcgaaccg gcacgagagc gaggccagcg ccgcccggtg
     1081 ctcctcaagc tccacgccct cggccaccac ttccttaccc agcttgttcg ccagaatgat
     1141 gatcgtctcg acgatgccgc ggctctcctt gtccgtgttc atccggctga cgaaggagcg
     1201 gtcgatcttg aggatgtcga tggggaagcg gtggaggtaa gagagactgg agtagcccgt
     1261 gccgaaatcg tcgatggaga gctgcacgcc gatggccttc atctgccgga gtatctcgac
     1321 ggcgccggcg gcgtcttcca tcagcgcgct ctccgtgatc tcgaggcgca ggcgctccgg
     1381 ctcgatgcct gagctgacga ggatgccgcg aatctcttcg acgaggtcaa tctgccggaa
     1441 ctgcttcacc gagatgttga cgctcaacgt caacgcgggg cggccgtcgt cgtccgggcc
     1501 ttgccagcgg ctcaactgcc ggcacgcctg ccggagaata gacatgccga gcgggacgat
     1561 gaggccggtc tcctcggcga gcgggatgaa gtcggccggg gagacgaggc cgcgctgcgg
     1621 gtggtgccag cgcgcgagcg cctcgaagcc ggtgacgacg cccgtctcca gcgagacgat
     1681 gggctggaag tgcgggcgta cctcgacggc ctcgatggcg tgccgcagct cgctctccag
     1741 cctgagcgcc tcgacggccc gcgtgtgcat ccccgtgttg aagacctcgt agcgcgcctt
     1801 gccgttggcc ttcgcgcggt acatggccgt gtcggcgtcg cgcaggatgt cttcgtggcg
     1861 gtcgtagccc gtggccgagt aggcgatgcc gaccgaggcg gtggtgaaga actcgtggcc
     1921 gtcgaggtcg aagggacggg cgagcgcctc ctgaatgcgg gcggccacgt cggtggcgtc
     1981 ggacggcgcg ttgatgccgt cgagcaggat ggcgaactcg tcgccgccga ggcgggccac
     2041 ggtgtccacc cttcggacgc agtcttcgag ccggcgcgaa agatcgatga ggaggcggtc
     2101 gccgaggtcg tggccgagcg agtcgttgac gaccttgaag cggtcgagat cgacgaagag
     2161 gacggcgaag cggtaggagg aggcgcgctt ggcgcgctcg acggcgaggc tcagacggtc
     2221 ggagagacgc gtgcggttgg gcaggcccgt gagcgagtcg tggaaggcgg cgtggtgaat
     2281 ctgctcctcg gcccgcttgc gatccgagac gttttgaatc tggaagatga ggtgccgggg
     2341 acggccgtcg gcgccgcgca cgagcgaggc cgactgcaac acccagatgg cgctgccgtc
     2401 cttgtggacg tagcgcgtct cgagctggta gctggagacc tcgccttcga ggagctggtg
     2461 catcttgatg agagattcgc cgaggtcttc cgggtgggtg acggcgcgga agctgcccgc
     2521 gagcatctcc gtctcgtcgt agccgagcat cgcgcagagc gagccgttga cctgaagcca
     2581 gtggcccttg gggctgatga gcgccatgcc gaccgcgtgg tcgaaggcgt tgcggaagtg
     2641 ctcctcgctc tccttgagcg cgcggctgat gcgcgcctgc tcggccatct gccggttcag
     2701 ctcctcgacg tggcgctgcg caagctcggc ctgcgtcgtg gccgactcga cgttcttcag
     2761 gtaggtggtg taggtgaagt agacgacggc gacgatgggc gccgtggcga ggaaggcgta
     2821 gacgccgagc gagccgatga gcttggcgat gatgccggcg gccgaggccc cggcgaagta
     2881 ggtgagcgaa gtccagagga agttgtcgcg ccacatccgc cagaccggct gcccgccgcg
     2941 cagcgccacg cccatcgcca ccagaccgga gttggcggcg tactgcacga agcccatgac
     3001 gcagatggcc atgacgtaga cggaggtgta gccctgctgc gtgagcgcgg gcagggggcc
     3061 gaacaggagt cgcagcgccc agagcgtgac gaacgtcgag cagatgtaga cggccgtgtt
     3121 gaagacgacc gtcagcttgt acttggagat gcgcgcggag gagaagaggg cgtcggctcc
     3181 ggcgaggagc acgccggcct cgccgccgaa gagcagcagc gaaagaagga tgaaggtgtc
     3241 cgagacggag atgtgccccc tcgtgcgcgg gatggcgacg acgatgcgcg agccgaagcc
     3301 gagcgtgatg agggcgaaga gcaggaaggg tagcccgagg tcaggaaccg aaattctcaa
     3361 cgcggagtaa gcggccgcca ccgcgcccag cgcgatgatc gtgaacataa aaggcttcga
     3421 cagaagctgc ctgcttgccg ggtccattcg tgtaccagag acgatgttga atgctgtgtg
     3481 agggcgtccg taaggggcat cggcggacta ccggcaggag aggttgcaaa cttcgttccc
     3541 cacggacgga cggccgcgtt agggcgaaaa cgcaggcaaa aacgtctcta ttactgaacg
     3601 gggccggcgg gggccgtggg agttcgttca aattgatgac aggaaatctc tcaacctgac
     3661 aagtcgcgtc aggcggcggc ccgggcggag cgtcgaataa aatccggcgg agagaggcgg
     3721 gccgcccggc aggggcgtcc aaatggggca ggctttacat caatttacat tcaggtatta
     3781 ccgtagatat ttgttgaaaa attcgagagt gcgcgtccac gccagtttgg cggccgtctc
     3841 gtcatagcgc ggcgtcgtgt cgttgtggaa gccgtgctgc ttgccctcgt acatgtgcga
     3901 cgtgtacacc ttcttgttgg ccttgagcgc cgcctcgtaa gccgctatcc cggcgttgac
     3961 ccgctcgtcg ttgctcgcgt aatgaaacaa cagcggcgcg gaaatcctgg ggacatcgtc
     4021 tacgcccgcc tggcgaccgt agaacgcgac tccggcgttc aggtcagcgc ccagccgcac
     4081 ggccaactga ttcaccatcc cgccgccaaa gcaaaatccg accgcgccca gtttgccggt
     4141 ggaatcggga cgcgccttga gccacttggc cgccgcgacg aagtcttcgg tcatcttcat
     4201 cccgtccacc tcgcgaaagg caacggcgcc tttctcgtcg ctgccggggt aaccgccgac
     4261 cgaggtcaac ccgtcgggcg cgaaggcgac gaacttggcg agcgcgaggc gacgcgccac
     4321 gtcttcgatg taggggttga gtccgcggtt ctcgtgaatg acgagcacga cgggcagctt
     4381 ctttttccct tgcgccgggc gggccagata ccctttgatc gagccgttcc cggcaggcga
     4441 cgggacgacc tcgtagccca ccttgatgcg tttgtcaccg ggcggcgcct gctgcgccca
     4501 ggcgtagttg ggtgtcagcc cctcgaagat tgccgcgacg gtcaggccgc cgaccgcgaa
     4561 cttggcggcg ccagcgagga aggcgcggcg gctgatgtcg ccgtgttggt attcgtgaaa
     4621 gaggtctaac aactcctgcg gataatcgga tgctgcttta cgttccattc ttcctccttg
     4681 gatggcataa agcggagtaa gagttaactc tcctacctcg ctggtgaatc cggcagctgt
     4741 tcattatggc gtcggcgcag gtttttcgat agcggaaaga gcagcgccag acacaggcat
     4801 aatatcaaca cacccgtcca gggtcacggc ctacgtgtgt ccgcgggggt gacgtgaggg
     4861 acgatgtgaa agctcagccg catctctcgt tggagccgat cacgttcctg cccgccgatc
     4921 ttgatttttc tgtagttcat tattgtttcg cgggcattcc attaatgttt cgcgggaact
     4981 tggcaaacga agctcgccgc ttcatccatg ctgccgctga taacactgcc cgcgcacacc
     5041 ctatcttttt ttacagataa tttaccgcgt cccgacaacg ccctctccgg ccccggtcat
     5101 catacggcgc attccggctt cgccacctcg caacacattt caggtaatta ctgctgagga
     5161 ggtcacaaga gtgttgagac gttttgtgag atgcgcctgc ttcggtctgc tgctcctgtc
     5221 gctcccggcc ctcccggcct tcgcgcagca gggcgcgggc gggccggcca gccccgccat
     5281 gcaggaggcc aacgcgctct acacggcgca gaaatgggag gaggccgcga aggcgtatga
     5341 gatgatcgcg cgcgagaacc cgaagagcgg ggtggcctgg tttcggctgg gcaactcgct
     5401 ccacgcgctc aagcagtatg agcgcgccat cgaagccttc cgccattccg tcgagatcgg
     5461 caacaactca accgcgatgt acaacatcgc ctgcggctac gcgctcatgg gccgcaaaga
     5521 ggaagctttc gagtggctct tccggtctct gaaggccggc ttcatcccgg cgggcgcggg
     5581 cctcgcctct gacgccgacc tcgcctcgct caccggcgac gcgaggttca aagaggcttc
     5641 cgccctcggc gagaagaacg cgcggccctg cacggtcacc ccggagcacc ggcagttcga
     5701 cttctgggtc ggcgagtgga acgtctacaa cccgcagggc acgcaagtcg ggacgaacac
     5761 gattgagaac gtcgtgggcg gttgcatcgt catggagaat tggacggggg cgggcggctt
     5821 cgtcggcaag agcatgaact tctacgaccc cgcgacgagg aagtggcggc agacgtgggt
     5881 ggacgggcgc ggcagcgtcc tcgatttcgc gggcgagttc aaggacgccg ccatgcgtta
     5941 cacgggcgag acggccggcg cgaacggcgc gcgcacgctg gcccgtctga cgttcttcca
     6001 tcttgggcct gaccgcgtgc gccagctctg ggagcagtcg tccgacggcg gcaagacctg
     6061 gagcgtggtg ttcgacggga tgtatctgag gaaggggacg gaaggcgcgg cgccgggcaa
     6121 gagcggcgcg aagtaagggc aaggcagggc tagggctgcg gcccttgctt acggaagaat
     6181 taacatggat gcacaggatg tgcaggataa gagatttgaa atctcaaatc tcaaatctga
     6241 aatttgaaat caatactttc ttcatcctgc ccatcctgcc catccatgtt gatttctccg
     6301 tagctcactc gcgcgcccgg cccgccgtca tggccttcgt cacctcgcgc atgcgcgcga
     6361 cgagcgcctc ggagtcgggc atacgcgtga cgacctgctc cagatggcgt agatactgcg
     6421 gcacgccgga ggccgcgcgc agctcttcca gaaacggctg caccttggcg aagaccgcga
     6481 cgtgctcgaa gaccgtgtcg ttgaacatct gctcgtcgat ggcgccgtgg ttgacgaagg
     6541 agcaggccat gtcccagtag ctcgtgacca tccggtagta ggcgctctcg tcgccgcgga
     6601 agacgttatg gatgtcctgc gcggaggtcg ggtggaagcc cacgaaccac gcgcgcgcct
     6661 gtcgcatcgt ttcttcccgc cgcagctcgt agagtctgag tatcccctcc atgctctcgt
     6721 acttgttggt caatgccgtc tctcctttcg ggaaatcgtt tggaagaact gaagcgtgtt
     6781 agcgcggacg ctcgacgcgg ccgtacttaa cgcggcgggc ttgccgtgag cgtcagtcgg
     6841 ccgacttcgt cagtcgttct ctgccgcagc agcagcgagc ggcacagcgc gaggttgcgg
     6901 cgcgcgtcct cgtatgcgtc gccaccttga gcgagcgcgg cggcgaactc gcactcggcc
     6961 tcgcggtgtc ggcccgcgag cgccagcatc acgccgaggt tgttgcgcgc cgtggggaag
     7021 cggtcgccgg aaatctccac ggcccgtttg aacagcgcgc cggcgcgctc gttcgcgccc
     7081 gcacgcgccg ccatgacgcc cagacgaaag tgcgcctccg cgtggcggcc gcccgacgct
     7141 ccgacggcct cgcggtaagc ggcctcggcc cccgcccact cttccaaatc ctcaagcgcc
     7201 acgccgagcg cgtaagacgc cttcgcgtaa ttgccgcgcc gcgccgcgac cgcctcgcgg
     7261 taggcggcga cggcctcgcg cgtgcgcccg agttcgtaga gacagacgcc gatgttgtag
     7321 cgcgccgtcg cgctcaactc gacggggccg gcgagcgcct tccggtaaga ctcgacggcc
     7381 tcctcgtaac gtccgcgcgc gaatttttcg ttaccctccc gaatggactg ccgcgcgtcg
     7441 tctctgtcgt ctggctctgc ggcgtgcccg gccgtgagtg ggaacaatgc gatgaagaga
     7501 acgaagagaa cagggatcag aatttttttc aacattgact cttacctccg cctgtcagtt
     7561 tgaatgcggg gcaagagtag cgcgcgggcg gctcacaaat ctttcacgtt gttgctggcg
     7621 tttgaccgtt cttgctactg ggtgcgcgga cgtactcgcg gcgtgcgcgg gaggcgccgc
     7681 ggtaagagcc gggcgtcgtg ccggtcagcc tgcggaacag acgcgcgaag tggctctggc
     7741 tgttgaagcc cgtgcggagg ccgacctcga caatcggcag acgcccctcg gcgagcaggc
     7801 ccttcgcgcg ctcgacccgg agcttgatga ggtactggtg cggggcctca cccgtcgaac
     7861 gcttgaactc acgtgcgaag tggaaagtgc tgatgccggc ctcgcccgcg aggtctgtga
     7921 gactcaggtc gtgcccgtag ttgtcggcca tgtacgcgag cacgcgccgc agggctggcc
     7981 ccgacagtcc gccggagaaa cgcgcggggg cggcggccga ggtcgtcgcc gcgtagtttc
     8041 tcagcagatg tatggccagg acgttggcga gcgactcggc gtagagcctg ccgtacaacg
     8101 cctcgccgcg ctccgactcg gcgaggagcg cctgcgcgac gttggcaact acagggtcat
     8161 atgcggcccg gcgttcgacg atctcgccgg cgcctgtgcc cgcctccgag gcggcgcggg
     8221 cgacgatcaa ggggtcgagg tagagcgaga cgcactcggc gtcgccgtcg agactgatgg
     8281 tgtgcgggcg gcgagcggga acgagaatca agctcccggc gcggttgcgc ccgccgacgc
     8341 ggaagccgtt cgccgtccgc acctcgccgc ggcagccgcc gccgagcgag acgatggcgg
     8401 cgtgctcctc gtgcgcgtgc tctgggaatt ggccccggcg caggcggtaa cgttcgagcc
     8461 ggatgccctg ccagcgtgtg gaacttttcg agacgaccgg cgactcctgt tcatcgtcca
     8521 acctgcattc atcctcgaac atgttttttc tccggggctt tcgtcttgcg cggactgcgc
     8581 ttcgggcgag gtctggtggc ttggggcgca tcggcggcgg agaggccgcg caggcgttgg
     8641 agaatcgcct cgcgggtctg cgcgaacacg cgcgcgtcgc cgtacaactg aaacgcccag
     8701 ttggcgccaa gctccagggc gttgatctcg aaggcgagtt gcgcgggcgt cacgtcgcgg
     8761 ctgagttcgc ccgcggcctg cgcctcgcgg gcggtgcgtt cgagggtggt gagccactcg
     8821 cgcatgatcg cggcgacgcg gtcgcgcacg gggccggggc ggccgtcgaa ctcggccgag
     8881 gcggcgaaga agaagcagcc gccgcggaag acctgaccgt tggcatagtc gagccacacc
     8941 tcgcacagcc tgcggagccg ggccagtccc cgcgtcgatt caaacgacgg atggatgacg
     9001 gcctcgatga agacctcgcg cgcggcctcg accgtggcga gctgcaattc ctctttcgag
     9061 ccgaagtggg cgaacagacc gctcttgctc atccccatct cggtggcgag gcggccgatg
     9121 gtcagcccct ccagcccctc ctcggaggcc acgtccacgg ccgcgcgcag gatggcctga
     9181 cgcgtgcggt ctccctttgc acggacggct ttagcggtgg cgctcgtggt cacgaatcct
     9241 cctccgaagc aacgaaactg ggcggcaata tagcacgaac gttcgtttta cgccagaggg
     9301 actttgacgt tgttattatg actttgcgtt tagagcgacg ttcacttgag tgaaaggaga
     9361 gtagattcac cgcggaggcg cagagggcgc agagactacg cagagggttg aacgattaac
     9421 tcaacggtga gtgaaaggct caaccctctg cgcgaactct gcgccctgcg gcgcctccgc
     9481 ggtgagttcg cgttccctac gctcgactca gagttagact acaactgcgt cacgggcgtc
     9541 gcggccggag acggcgtcgg cgtcggcgac gggctggggg tcggcggcgg ggccggcatt
     9601 cgctccttcg tgaagcgggg cagcggctcc tcgcgcgagg cgaggtggta gacggccgcg
     9661 gcgatgacga cggccgccgc cttcacgtcg tcttcgacga tgcgctcgta ggtgtcgagg
     9721 ttggtgtgcc aggtgtgcgt gccgtactcg atggggtctt gccccatgcc gatgccgggc
     9781 agtcccgact ggttgaagga ggtgtgatcg ctgccgccga ggttccggct gcgcgtggcg
     9841 atggctccct cgacgccgag gtcggcgaac ggcaggagcg cctgccgcag aatcgtcgcc
     9901 gcctccggcg ggccgaacac ggtcgcgccg cgcacgcgcc ccgtccccga gtcgatgttg
     9961 aaatagccgt tgaacttcga gtagtcgggc ttcggattct cggcggtgcc gaagtgctct
    10021 ttgatgtatg cctgcgagcc gagcagcccc tgctcctcgc cgctccagag cgcgacgcgg
    10081 atggtgcggc ggggcttggc gccgatggcc ttcaggatgc gcgcggcctc catcatgatg
    10141 gcgcacccca cggcgttgtc ggtcgcgccc gtcgccgcgt gccacgcgtc gaggtggccg
    10201 ccgagcatga cgacctcgtc gcgttcgccg tcgcccggta tctcggcgac gacgttgtag
    10261 gaagtgcggc cctctgggta agtgcggttg acgatgttga attcgagttc gaccggcgcg
    10321 gcgtcggcga gcagacgcga gatgcggccg tagtcttcat tacgcatgac gaccgtcggc
    10381 acggccttcg tcacgtcgaa ggtgcggttg ttgaaagcgc gtatctgccc gtgctcgcgg
    10441 ccggcgtcgt tgacgcgcac cagcgcgccg ttatcgacga ggaactgctc gacggcctcg
    10501 gccacgcgtt gcggggggag cggctggtcg gggccgggcg tgggagtcgg cgtcggcgac
    10561 gggaagacga acgggggcgc gggcggcgtg ttcgggtcga aggcgttgcg cgcatcctgc
    10621 tcgtcgcggc gcttgaaggg cgggttgaac gagacgggca ccgtctggtg tcggccgacg
    10681 aggacgatgc cgcctctgac gcgctcgcgg atgctgttca ggtaggcgtc gaggcgctcg
    10741 cgtgtggggc gctcgggcgt gatgagttgg aaggcgcggc cagtgaccgt gccgttggtg
    10801 ccgggcgtcc aggccagcgc ctcgcagacg agcgcgtcct tgacgggcgc gacgatgtgc
    10861 gccgacaggc gttcgttctg ccagccgggg tgcttgaagt cccacggctc aaggcgcgcg
    10921 ttcttcatgc cccacgactc catctgcttc gccgcccact cggccgccgc cttgtggctg
    10981 ggtgagccgg tgaggcgcgg gccgtagagg tctgtcagga agtggatcgt ccgcatgatc
    11041 tgcgagttgt ccgtcccctc gcgtctgatc ttccagaaga cttccgcgtt gatgcgctcc
    11101 gtccgagcgg cggccggctc ctgtcccccc gcgggaagcg ggacgtagat gcaaacgaaa
    11161 accagaagaa ttgcaagacg tcttttcaac atcgcttggg ctccagtcgt gtaaagagtt
    11221 cccgtcgccg caggggtgtt gcgtacctgc cgcgcggccg aatgttttta gcggttagcg
    11281 agtggttata acactccggc gggcggttga gaaagccgac gcgccgggtt gtccgccggt
    11341 gcggtaaagc ggcgcggaag cggttgacga aaattttcgt tgacaatgac gaagactttc
    11401 gtcaataatg ccggcgcccg agaggagagg tgcccgagaa tgcgtagagt gaaatcgaaa
    11461 cagccgctac ggcgaccgac cgagttggag ttgtccatcc tgcaagtgct ctgggggcac
    11521 ggccccggca ccgtccgcga agtgctccaa cgcttcaacg acgggcgcgg gagcgaggcc
    11581 ggctacacga ccgtcctcaa gatgctccag atcatgacgg agaaggggct ggtcgaaaga
    11641 gacgacgcgc gccgcccgca ggtttaccgc gcgcgtctgt cgcaggagca gacacagcgg
    11701 cagctcgtca gcgacctgtt ggagcgggcc ttcggcggct cggccaagca gctcgtgatg
    11761 caggctttgg cgacgcgcaa gacctcggag gcggatttga cgcagatcga gaagctactc
    11821 gacaagctcg aaggaggcac ggagtgaaca acctcgaaga cgttttgcag caaacagttt
    11881 ttcatgccgt cggctgggcg ctcgtccact cgctctggca gggcgcgggc gtcgccgcac
    11941 tactcgccgg cctgctcgcg ctcctgcgtg ggcgacactc gcgtgcgcgt tacgccgccg
    12001 gttgtgcggc gctcgcgctg atgctcctgc tcccggcgac cactgtctgg aagatgagcg
    12061 gcggcagcga aagcgcgcac gcagcgtccg acgagacggg acgccggcgc gagaaccatg
    12121 cgaggacggg cacactggag gcgaccgtca tcgccgccgg agcggtcgag gacgcgccgg
    12181 agggttttgc ctacgagagc cacgatgccg gccggcatac ctcgacgggc ggcaggttcg
    12241 agggcgtgct gccgtggctg agtctgctgt gggcgttggg cgtggcggtc ttctcggcgc
    12301 gcagccttgg cgggctaatc tacgcgcgac ggctcaaaag cgtggggacg cgcccggcgg
    12361 cgcgtgagtg gcagcagcgc ctcgcggagc tttcgcggca gttgcgcgtg aagcgtgcgg
    12421 tgcggctttt ggagtccacg ctcgtgcagg tgccgacggc cgtcggctgg ctccggcctg
    12481 tcgtcctcct tccagcgagc gcgctcacgg gactcacgcc gcgccagctc gaagtagtgc
    12541 tcgcgcacga actcgcacac atccgccgac acgactactt agtgaatcta ctccagaccg
    12601 ccgtcgagac tttgcttttc taccacccgg ccgcgtggtg ggtggcgggc cgcgtgcgcg
    12661 ccgagcgcga gcacgtctgc gacgacatgg ccgtgtccgc gacgggcggc gacgcactgg
    12721 gctacgcgcg cgcgttgacg gcgctggagc gcctgcgccc gcacgcgcgt ccgcaactgg
    12781 cgatggcaat cgacggcggc tctctgcgtt cgcgcatcat gcgtctcgtc ggtgcgccgg
    12841 ccgcatcgcg ccgcacgtcg ccgctgctgg cgggcgtcgc cttcgccgcc gcgctcatta
    12901 ccaccgtggc gtgcacgggc gctgtcctct cgcagaataa gaagcctgcc ggcaactccg
    12961 gcaacgagcc gggcgcggcg aagggcgagg ccgcgaacgc ggcgcgggag aagtcgaacg
    13021 cggaggttgc gaacctgtcg ccggcgatgc agaagctcgt ggcgggcgac tcgacggagg
    13081 gcgaggacac ggaggtgcgt cgcgccgcgc tcgccgcgct tggcaagcgc aacggctcgg
    13141 tcgttgtcat ggacccttcg acgggacgcg tctacgcggt cgtcaatcag gagtgggcct
    13201 tgcggcgcgg ttggctgccg gcctcgacga tgaaagtcat cgtcagcatg gccggtctcg
    13261 gcgagcggac gctcgacccg gcgcagaagg tgaaagtctc tgaccgcccc gagcgcatca
    13321 acctgacgga cgcgctggcc ttctccaaca acccttactt cttccccgtc agcgagcgcg
    13381 tcgggtcgga agccatcctc gcctacgcgc gccggctcgg actcggcgag ccgaccggca
    13441 tcaactacga gggcgagacg gcgggcatcc tgccggcctc ggcgcgcggc acgaacgccg
    13501 gccggctcgg cacgcacggc gagggcgtgg aggtgacgcc cgtgcagttg gccgcgctca
    13561 cctcggccat cgccaacggc gggacgctct acgtgccacg cgtgccgcgc acggcggaag
    13621 aggccgcgcg cttcgagccg aaggtgcgcc gctcaatcga catcccgcgt gagattttag
    13681 accgcatcac gccggggctg ttggcgacgg tcgagcgcgg gacaggcgtg ggcgcgaagt
    13741 tggaggcgct taaggtggcg ggcaagaccg gctcggccgg gggcaagaac cacagccacg
    13801 gcatctttta ttcctacgcg ccggcggagc gcccgcgcct cgtcgtcgtc gtcgttatgg
    13861 aaggccccgc agagaacggg cgcgagtcgg ccggtgtcgc cggggcgatc tacaaggctc
    13921 tcggcagccg gctcgcgggc ggcgagtgaa gcgcgcgcga gcggcaacga gtcaaatact
    13981 ttggagcgcg acgcatgact cgtggccgag tggattcgac ggttgattca ccgcagaggc
    14041 gcaga
//
LOCUS       DASVES010000014        13352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_771821, whole genome shotgun sequence.
ACCESSION   DASVES010000014 DASVES010000000
VERSION     DASVES010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13352
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771821"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF240_00605"
     CDS             <1..220
                     /locus_tag="VF240_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173769.1"
                     /translation="AVRALEKAIDIDPDFFRAAEALTRAREGLDRRKAFLKDLEKMRQ
                     KPKTKSNTNANTNASSAPAPTPRPPSTP"
     gene            complement(287..1156)
                     /locus_tag="VF240_00610"
     CDS             complement(287..1156)
                     /locus_tag="VF240_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7173770.1"
                     /translation="MLRTKPDTNAQRPRLGLALSGGAARGVAHVGVLKVLDAEGIQVD
                     CVAGTSAGALVGGAYAAGMPIDELVELGRGLRWRDFGRMTLSRLGVQSNARMEEYIRE
                     RFPVVRFEEMRLPFAAVATDLHTGLPVVMRDEGDVAFAVRASCAVPGWYVPVTDERGR
                     QLVDGGLVANLPSSVARSLGADIVVAVDVNYEGARFLGPPQSAIGVLFQTFLVVQRTA
                     AAHQRQAADVCISPHVGHIRWDEMGRADELIAAGVEAARAALPAIREFLAPTPAEPAP
                     RWFSALRRGRRSA"
     gene            complement(1183..1836)
                     /locus_tag="VF240_00615"
     CDS             complement(1183..1836)
                     /locus_tag="VF240_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173771.1"
                     /translation="MVAVINGRELITHTDLLWQLALQPAAALDNPRREDLGRALQLVI
                     DQRLIAQEAEKLPSVAATDAEVQAELKKLVGRFPSGEEFYARLRRVGLGEDSEQLREI
                     VRQRVAIDKYISFRFRAFTVVSPEEVADYYRDVWAPRRRRQAPGSVAPRLEDVSAEVE
                     EELRENKIESDTDEFLEDARATAQIVILDQAAVNGGQGTGAGGQGPAKATVLPTPVP"
     gene            complement(1976..5392)
                     /locus_tag="VF240_00620"
     CDS             complement(1976..5392)
                     /locus_tag="VF240_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7173772.1"
                     /translation="MNARSIFVVLLLLAAAAPALARGAGVRVDAVVRNVVDLSKYEGR
                     TVATVEVVAEGAQPAEAAVSELRSRVRVAEGSRFSVVRVRESLLELYASGRVADARVE
                     ASDAPGPAGEDGRPRLALRFVVRPQVLVAGVEFEVGVTAGTEVTEDELRSRLTLLDPG
                     RPVTEQALRTSADAIQVYLRDRGFYRAEVTAAQQLDPTRTRATVVFSVAPGESTLLEN
                     FNINITGFDAARVAADLTLQRGARFTQESLGQDIARIRQAIIETEHLAPRIDEPRITL
                     NSETNRINVELTGAVGPRVKAAVTGYEGLSEKRQRELLPILREGTIDYSAIVEGKRRL
                     ENRLQEQGYFFAEATPVCTIVPPLLAAPGGNGSAAAPASPTAAQAVVALAQGDELGAC
                     ETMPGPEELTDRSVSITYEVAAGRRFKLTDIRVTGTERLSEVDIEDLKDELRSREANA
                     LGIIPLIGFGRGFTSTDALEQDRRIVQARMRELGYRRAEVAVLQGVSLEGDALIITFD
                     VTEGPLTRVAGVEVRGNQIFTAEEVAGERCPREPLPDEVCLIEGGAYSRRQARTDGDR
                     VRGFYARRGYVDAEVDVSVVELPRASGGDEQVRLIYTVKEFEKVFVNEIFVNGLVHTK
                     KTAVLDAIPLRRGEVLRADDIQESERILVGQTGDAFRQVVIRTEAAGETASGFPKRDV
                     IIDIEERKRIVMDYGGGFSTDNGPLGLFEIRNSNLFGDLKQGALRSRASRRQQLLRLE
                     YFDPRFRRYGVKDFSPLTFSVQYTRDTSVTRFFRSTIDRGTGGIVQRFDEEGKIIDEF
                     GARVKEPSINRFQINLETQRDLELELGPRGEVRKRSTLFLRYNYEDVRLFNIGSLLLA
                     PELRPDRAVRLSRFGVAFARDTRDRQFDPTRGELLRVDYGLALKALGGNLSFSKLLTD
                     YSRYYKFNRVRETVLAARVQLGLARIYDPPDRNDNGVTDDVDRRLPISERFFSGGSTT
                     LRGFSYEEAGPRVVAPECFFSATPAAPAPCGVFRNQKGEVVPLNPFTVPIGGNALAVV
                     NLEARVGLTRNLQAVPFYDGGNVFRRVGDIFKKGCDIDPADPNGQNLCAEWSHTVGLG
                     FRVKTPLGPFAVDYGYVLNPREFIIPQFGGATAIHRLSRTQIHFRFGQTF"
     gene            complement(5496..10481)
                     /locus_tag="VF240_00625"
     CDS             complement(5496..10481)
                     /locus_tag="VF240_00625"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX7173773.1"
                     /translation="MPVDEHDDSAPNAKGPEPAGGEPVKFTEASAGGDDAPAPLTPRG
                     RRARWRRVVNRRNAMWTGIAAVAAVVLLVIVAVILYRTGQVDRIIARQIVETFSEYGI
                     RAEIGSFETKFGPRTAEIKDVKLYDAQTGAALGEIERILATVRIEDMYALSLTRDVKL
                     EAMELDGLKLWVVFDEQGRSNFSNLRLPAPDPNSRILFSYSTANIKINSSVIHFDDRQ
                     HSLSGEARNLRATVRPDDPNAPEESRMNVFEIAMSDSTFVYDGRPVNDIDIEARGRAN
                     QTRAEVSELVLRSPVAEARLTGTLDDWRALRYQMKVEATVDLTQTSDVLRAETTLRGS
                     GRFDGAVSGEGDNYKVVGQLTSDALAADGVRLQALNVNATGTGTGKTYEVQGKAVAEL
                     LTAGDYRINGVQLAGGVRGTGTDFRWLGDLRAAAVRGGQTGIGDLIVSDAVAEFKEGR
                     LEGTAGGVSARTLTTGDTAASGVRAGGVRFRQDEGGGFRATAESASVASVKDQDTVVS
                     DIRASNIDATVRPDESADVTVGRVSLGSVAASQYRTGGLNIAGVRLSVSPGGVIEGTT
                     SDIDAGTVTLAQGGRAEQVRLGRPRFQLEPSGRYRASADLSLGGGVLGEMRLGSLRGE
                     VTATNSSAELKNFVAEIFGGSARGNASFATGRGGASRIAAAFEGVDVGGLVAATSGSV
                     VPLTGAATGTVDLSFPGTNYKAASGKLNAKFTGETGQEATGRTPLVGELALSAERGVF
                     EVERGNLRAGSTELSATGRFSFAGGSDLAVGVNSADASEFQRVLVSTGLLPDVEAQLA
                     RFGVELAGKLSFNGTLRGDFDAPTVNGRFEVASLRARGQDLGALSADIESDAAETRVK
                     NGQLVEPDGGGARFSATIPRTGENNIAVEATLENANAGRLLAALGGDVRTSADAGPLI
                     NPQSVAAMGPVSGELNVTGLPGAMSGSAELRAAPGVIGSQRYEELAARLTFGGKKVNI
                     ERLSARFEAGAVEASGTVDTDTQEFDLRARGQGVRLDLVTDLFGGGGAGVARRLPALA
                     GTADFTATASGRLDNPRTYRVEVDAKGQNVTINGQPAGDLALTGRTTTDNKFTLELVT
                     GVLGQPQTLRAEIDLASNELATTVETTLTGADLAQLFRVLLPDSGVTVTGRATGTLRA
                     TGNLFDDEGAFSIAGLQGRAEFTELVVNIEDVPLAAENPLVVLFSTNEVTFERTRFTG
                     PGTDIVFGGTAALGAAGRQNLSVDGSLNLRAVRLSPNTFMGGTARVGVRVTGSFSEPR
                     ITGTAEVTNGSLATLIQDERLQVTQIVARVRFNADQAQIETLTGRLGGGRVSIAGGAR
                     LEGLRPAQFRFNVRGDNVTVPFPEGFRTTADTDDLEVSSTREGEQLVKGTVRLRRVEY
                     TEDIDLADLLDNRREPTIEEGISTEGALGQTILDLQLEGRDALVVRNNLGDLVGSVSL
                     SARGPVDDPVISGRITATRGTLNFRNNRFEIQRAIVDLPPRREADPVLNIVAEGEIRG
                     YRITVTPTGPLSQLQVTLRSDPPLPQSDVVSLITTGSLSSGDQSASVLGQSGVGTATS
                     LLTDSLINAPVRRATDKLFGLNRFEIDPLIVGRGGASPTARLTVGRQINRNLSVTYST
                     NVTGEQNQVLALEYRVSDRLSFVAQYQQGSQNTLRTQNNDFSFEVRFRKRY"
     gene            complement(10620..11786)
                     /gene="ribB"
                     /locus_tag="VF240_00630"
     CDS             complement(10620..11786)
                     /gene="ribB"
                     /locus_tag="VF240_00630"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX7173774.1"
                     /translation="MPFASIEEAARDLSDGRMVIIVDDEDRENEGDLACAAECVTPEM
                     INFMATHARGLICMPLTEERCDELQLTMQVADNTSFLGTAFTVSVEARRGVTTGISAS
                     DRATTILTAVDPRTRPQDLARPGHVFPLRARTGGVLVRTGQTEAIVDLARIAGLYPAG
                     VICEIMNPDGTMSRMPELKKFARKHGLKIISVADLVRYRMRKEVLVRRAVETDLPTVY
                     GRFRAVAFENIVNGDVHLAMVMGDVRTDEPVLVRVHSENVTCDMFGTLVDDTGHQLHA
                     ALERIAAEGRGLVLYLRQRDNNLDLVHQLRTYQIMQERGLTKTEACLETGYGVERDYG
                     VGAQILHELGLRRIVLLTNHPPKVSALEGFGLEIAGSVPLGSPARAEKQMLNAE"
     gene            complement(11921..>13352)
                     /locus_tag="VF240_00635"
     CDS             complement(11921..>13352)
                     /locus_tag="VF240_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173775.1"
                     /translation="DGNWVIYCTLAVIKYSDWATDSTFGQVNSESQFGVALAYTDDDL
                     FDCFRIANLSSLLDNIVGLNRWAGKGAVVLVGADGFPVVEADDAVLEAVRHYERRRPK
                     DGLLEDQGLHVKAPVQAGADNIILLNRLRRPIPLAFPKNNFTLFLRNYLPHPTATASL
                     ESSLRAYEEALVDFLGAGSEAIFGTLRSLSSRVWQTTPALKAVDGAIICDLDVEGDPA
                     AHHVVRFFFDLSQKGYLRMPEAVLRERLSDRVGFPDGFSPAQASLLVNTFFDRFLLTK
                     ETRDSIDVLALDPCPLVYTSPGGNCYFDLLLIPDFLHWLFVKGREWFSSQHGDRFTLS
                     LKSLVEQSGSGAEVVGAKRYFQAADGKRAEVDLLVRKGETLYAVECKAYSKSREYWLG
                     NYDAVTARSGRIGAAVRQATETAVTIAEIIRDGGVGAFGCTRVEWVLCLPAQEFLIPI
                     DKYGMLTAEIPKVCTPEEFVTYLSVP"
BASE COUNT         2144 a         4349 c         4664 g         2195 t
ORIGIN      
        1 ggccgtgcgc gcgctcgaaa aagccatcga catcgacccc gacttcttcc gcgccgccga
       61 agcgctcaca cgcgcccgcg aaggactcga ccgccgcaag gcattcctca aagaccttga
      121 gaagatgcgc cagaaaccca agacgaagtc caacacgaac gccaacacga acgcctcttc
      181 ggcgcccgcc ccgaccccgc ggccgccctc aacgccgtaa gcacttcccc gctggggaag
      241 cacgaagcgg tgaatctact ttccttccac tcaagttaaa ctcgctctaa gctgaccgtc
      301 ggccgcggcg cagcgcggag aaccagcggg gggcgggttc ggcgggggta ggcgcgagga
      361 attcgcggat ggctggaagc gcggcgcgtg cggcctcgac gccggcggcg atgagttcgt
      421 cggcgcggcc catctcgtcc cagcggatgt ggccgacgtg ggggctgatg cacacgtcgg
      481 cggcttggcg ttggtgcgcc gcggccgtgc gctggacgac gaggaaggtc tggaagagga
      541 cgccgatggc cgattgcggc gggccgagga agcgcgcgcc ctcgtagttc acgtccacgg
      601 cgacgactat gtccgcgccg agcgagcgag cgaccgacga cggcaggttg gcgacgagtc
      661 cgccgtccac gagttgccgg ccgcgctcgt ccgtgacggg gacgtaccag ccggggacgg
      721 cgcaactggc gcgcacggca aacgccacgt cgccctcgtc gcgcatgacg acgggcagcc
      781 ccgtgtgcag gtcggtggcg acggccgcga acggtagccg catctcctcg aagcgcacca
      841 cggggaaacg ctcgcggatg tactcctcca tgcgcgcgtt ggactggacg ccgaggcgcg
      901 atagagtcat ccgtccgaag tcgcgccagc gcagaccccg ccccagttcc acaagctcgt
      961 cgatgggcat ccccgccgca tacgcgcccc cgacgagcgc gcccgccgag gtgcccgcga
     1021 cgcaatccac ctgaatgcct tccgcgtcga gaaccttgag cacgccgacg tgcgccacgc
     1081 cgcgcgccgc gccgcccgag agcgcgaggc cgaggcgcgg cctttgggcg ttcgtgtcgg
     1141 gtttcgtccg cagcataaaa cgggaagacg ggggccgcgc ggtcagggga cgggggtcgg
     1201 caagacggtg gctttggccg ggccctgtcc cccggcccct gtcccctgcc ctccattcac
     1261 cgccgcctga tccagaatga cgatctgggc cgtcgcgcgc gcgtcttcca aaaactcgtc
     1321 ggtgtcggac tcgatcttgt tctcgcgcaa ctcctcctcg acctcggccg acacgtcttc
     1381 aagtctgggg gcgacgctgc cgggcgcctg acggcgacgg cggggcgccc acacgtcgcg
     1441 gtagtagtcg gccacctcct cgggcgagac gacggtgaag gcgcggaagc ggaaggagat
     1501 gtacttgtcg atggcgacac gctggcggac gatctcgcgc aactgctcgg agtcttcgcc
     1561 gaggccgacg cggcgcaggc gcgcgtagaa ctcctcgccc gacgggaagc ggccgacgag
     1621 cttcttcagc tcggcctgca cctcggcgtc ggtcgcggcg accgagggaa gcttctcggc
     1681 ctcctgcgcg atgagtcgct ggtcgatgac gagttggagc gcgcgaccga ggtcttcgcg
     1741 ccgcgggttg tcgagcgccg cggcgggctg gagcgcgagc tgccagagca ggtcggtgtg
     1801 cgtaatcagc tcgcggccgt tgatgacggc caccatgcgg tcaacgacct cttgagaaag
     1861 gcaaacgtaa aaaggcaaaa ggcaaagggc cgggagcagg actagcctcg aataccagca
     1921 gcacgacttg tttctcgctc ttttcacttt tgccttttgc ctttttactt ttgccttaaa
     1981 aggtctggcc gaagcggaag tgaatttgtg tgcggctgag acggtggatg gccgtcgcgc
     2041 cgccgaactg cgggatgata aattcgcgcg ggttgagcac gtagccgtaa tcgacggcga
     2101 acgggccgag aggggtcttg acgcggaaac cgaggcccac ggtgtgcgac cactcggcgc
     2161 agaggttctg cccgttcggg tcggcggggt caatgtcgca gcccttcttg aagatgtcgc
     2221 cgacgcggcg gaagacgttg ccgccgtcgt agaagggcac ggcctgaagg ttgcgcgtga
     2281 ggccgacgcg cgcttcgagg ttgacgaccg cgagcgcgtt tccgccgatg gggacggtga
     2341 agggattcaa ggggacgacc tcgcccttct ggttgcggaa gacgccgcag ggggcgggcg
     2401 cggcgggcgt ggcgctgaag aagcactcgg gcgcgacgac gcgcgggccg gcctcctcat
     2461 agctgaagcc gcgcaacgtg gtcgagccgc cggagaagaa gcgctcgctg atggggaggc
     2521 ggcggtcaac gtcgtcagtg acgccgttgt cgttgcggtc gggcgggtcg tagatgcggg
     2581 cgaggcccaa ctgcacgcgc gcggcgagga cggtttcacg cacgcggttg aacttgtaat
     2641 agcggctgta gtcagtcagc agttttgaga aggagaggtt gccgccgaga gctttgaggg
     2701 cgaggccgta gtctactctg aggagttcgc cgcgcgtcgg gtcgaactgg cggtcgcgcg
     2761 tgtcgcgggc gaaggcgacg ccgaagcgtg agagacgcac ggcgcggtcg ggacgcagct
     2821 cgggggcgag gagcaggctc ccgatgttga agagacgcac gtcctcgtag ttgtagcgca
     2881 ggaaaagagt gctgcgcttg cggacttcgc cgcgggggcc gagttctaat tcgaggtcgc
     2941 gctgcgtttc gagattgatc tggaagcggt tgatcgaagg ctctttgacg cgcgcgccga
     3001 actcgtcgat gattttgccc tcctcgtcga agcgttgcac gatacctccg gtgccgcggt
     3061 cgatggtcga gcggaagaag cgcgtaacgg aggtgtcgcg cgtgtactgg acggagaagg
     3121 tgagcgggga gaaatccttg acgccgtagc ggcggaagcg cgggtcgaag tattccagac
     3181 ggagcaactg ctggcggcga ctcgcccgcg agcgcagtgc tccctgcttg agatcgccga
     3241 agagattgct gttgcgtatc tcgaagagac cgagcgggcc gttgtcggtc gagaagccgc
     3301 cgccgtagtc catgacgatg cgcttgcgct cttcgatgtc gatgatcacg tctcgcttcg
     3361 gaaagccgga agccgtctcg ccggcggcct cggtgcggat gacgacctgt cggaaggcgt
     3421 cgcccgtctg cccgacgagg atgcgctcgc tctcctgtat gtcgtcggcg cgcagcacct
     3481 cgcccctgcg cagggggatg gcgtccaaga cggccgtctt cttcgtgtgg acgaggccgt
     3541 tgacgaatat ctcgttgacg aagaccttct cgaactcctt gacggtgtag atgagacgca
     3601 cctgctcgtc gccgcccgag gcgcggggca gctcgacgac ggagacatcg acttcggcgt
     3661 ccacgtagcc gcggcgcgcg tagaagccgc ggacgcggtc gccgtcggtg cgcgcctgcc
     3721 ggcgcgagta ggcgccgccc tcgatgagac agacctcgtc gggcagcggc tcgcgcgggc
     3781 aacgctcgcc ggccacttcc tcggccgtga aaatctggtt gccgcgcacc tccacgccgg
     3841 cgacgcgcgt gagcggcccc tcggtcacgt cgaaggtgat gatgagggcg tcgccttcga
     3901 gcgagacgcc ctgcaggacg gccacctcgg cgcgccggta gccgagttcg cgcatccgcg
     3961 cctgcacgat gcgtcgatcc tgttcgagcg cgtcggtgct ggtgaagccg cgcccgaagc
     4021 cgatcagggg gatgatgccg agggcgttgg cctcgcggct gcgtagctcg tccttgaggt
     4081 cttcgatgtc cacttcggag agacgctcgg tgccggtgac gcggatgtcg gtgagcttga
     4141 agcgtcggcc ggcggcgacc tcgtaggtga tggagacgga gcggtcggtc aactcctcgg
     4201 ggccgggcat ggtctcgcag gcgccgagtt cgtcgccctg cgcgagcgcg acgacggcct
     4261 gcgcggccgt gggcgaagcg ggggcggcgg cgctgccgtt gccgccgggc gctgcgagaa
     4321 gcggcgggac gatggtgcag acgggcgtgg cctcggcgaa gaagtagccc tgctcctgaa
     4381 ggcggttctc cagacggcgc ttgccttcga cgatggccga gtagtcgatg gtgccctcgc
     4441 gcaagatggg cagaagctcg cgttgccgct tctcgctcaa gccttcgtag ccggtgaccg
     4501 cggctttgac gcgcgggccg acagcgcccg tcagctcgac gttgatacgg ttggtctccg
     4561 agttgagcgt gatgcgcggc tcgtcgatgc gcggcgcgag gtgctccgtc tcgatgatcg
     4621 cctgccggat gcgcgcgatg tcctgaccga gcgactcctg cgtgaagcgc gccccgcgct
     4681 ggagcgtgag gtcggcggcc acgcgcgcgg cgtcgaagcc ggtgatgttg atgttgaaat
     4741 tttcgaggag cgtggactcg cccggcgcga cgctgaagac gacggtggcg cgcgtccggg
     4801 tcgggtcgag ctgctgcgcg gccgtgacct cggcgcggta gaaaccgcgg tcgcgcaggt
     4861 agacctggat ggcgtcggcg ctggtacgca gagcctgctc ggtgacgggc cgtccggggt
     4921 cgagcagcgt gagacgcgag cgcagctcgt cctcggtgac ctcggtgccg gccgtcacgc
     4981 cgacctcgaa ctccacgccg gcgacgagca cctgcgggcg gacgacgaac ctgagcgcca
     5041 agcgcggccg tccgtcttca cccgcggggc cgggcgcgtc cgacgcctcg acgcgcgcgt
     5101 cggccacgcg ccccgacgca tagagttcca acagcgattc gcgcactcgc acgaccgaga
     5161 agcgcgaccc ctcggcgacc ctcacgcgcg agcgcagctc cgagacggcc gcctccgccg
     5221 gctgtgcccc ctcggcgacc acctccaccg tcgccaccgt ccgcccctcg tacttgctca
     5281 gatcgacgac gttgcgcacg acggcgtcaa cgcgcacgcc cgcgccccgc gcgagcgccg
     5341 gagcagcggc ggcgagaagc aaaaggacga cgaagatgga gcgagcgttc atcacaaaca
     5401 ggagtcagaa gtcaggagtc aggagtcaga atccgaaatg tcacggcccg tgttttcatt
     5461 ctgactcctg actcctgact cctgacttca tccccctagt acctcttcct gaaccgcacc
     5521 tcgaagctga agtcgttgtt ctgcgtccgc agggtgttct gcgagccttg ctgatactgc
     5581 gcgacgaagg agaggcggtc ggagacgcgg tactcaagag cgagcacctg attctgttcg
     5641 cccgtgacgt tggtcgagta agtcaccgag aggttgcggt tgatctgccg gcccacggtg
     5701 agccgggccg tggggctcgc gccgccgcgc ccgacgatga gcgggtcgat ctcgaagcga
     5761 ttgaggccga acagcttgtc ggtcgcgcgc cggacgggcg cgttgattaa actatccgtc
     5821 aaaaggctcg tcgccgtccc cactcctgac tgacccaaca ccgatgcact ctggtcgcca
     5881 gacgagaggc tgcccgtcgt aatcagcgag accacgtccg actgcggcag cggcgggtcg
     5941 gagcgcaaag tgacctgaag ctgcgagagc ggccccgtcg gcgtcaccgt gatgcggtag
     6001 ccgcgaatct ctccctcggc gacgatgttc aagacggggt cggcctcgcg gcgcggcggc
     6061 aggtccacga tggcgcgctg aatctcgaag cggttgttgc ggaagttcaa ggtgccgcgc
     6121 gtcgccgtga tgcgacccga gatgacgggg tcgtccacgg ggccgcgcgc gctcagggaa
     6181 acggagccga cgaggtcgcc gaggttgtta cggacgacga gcgcgtcgcg gccttcgagc
     6241 tggaggtcga ggatggtctg cccgagcgcg ccctcggtgc tgatgccttc ctcgatagtc
     6301 ggctcgcggc ggttatcgag gaggtcggcg aggtcgatgt cttcggtgta ctcgacccgg
     6361 cgcaggcgca cggtgccctt gacgagttgc tcgccttcgc gcgtgctgct gacttcgagg
     6421 tcgtcagtgt cggccgtagt gcggaagcct tcggggaacg ggacggtgac gttgtcgccc
     6481 cgcacgttga accggaactg tgcggggcgc aagccttcga gacgcgcgcc gcccgcgatg
     6541 gagacgcgcc cgccgccgag gcggcccgtg agggtctcaa tctgcgcctg atcggcgttg
     6601 aagcggacgc gcgcgacgat ctgcgtcacc tgaagacgct cgtcctgaat gagcgtggcg
     6661 agcgagccat tcgtgacctc ggccgtgccc gtgatgcgcg gctccgagaa gctgcccgtg
     6721 acgcgcacgc cgacgcgcgc cgtgccgccc atgaaggtgt tgggcgaaag acggacggcg
     6781 cgcaggttga ggcttccgtc aacgctcaga ttctgccggc cggcggcgcc cagcgcggcc
     6841 gtgccgccga agacgatgtc cgtgcccggc cccgtaaagc gcgttcgctc gaacgtcact
     6901 tcgttggtcg agaagagaac gacgagcggg ttctcggccg cgagcggcac gtcttcgatg
     6961 ttgacgacca gctcggtgaa ctcggcgcgg ccctgtaggc cggcgatgct gaaggctccc
     7021 tcgtcgtcga agaggttgcc ggtggcgcgg agcgtccccg tggcgcggcc cgtgacggtg
     7081 acgcccgagt cgggcaggag cacgcggaac agttgcgcga ggtcggcccc cgtgagcgtc
     7141 gtctcgacgg tggtcgccag ctcgttcgac gccagatcga tctcggcgcg tagcgtctgc
     7201 ggctgtccga gcacgcccgt cacgagttcc agtgtgaact tgttgtcggt cgtggtgcgg
     7261 cccgtcaaag cgaggtcgcc ggccggctgg ccgttgatgg tgacgttctg acctttggcg
     7321 tccacctcga cgcgataggt gcgcgggttg tcgagccgcc ccgaggcggt ggccgtaaag
     7381 tcggccgtgc cggcgagcgc gggaaggcgg cgggcgacgc cggccccacc gccgccgaag
     7441 agatcggtga cgaggtcgag ccgcacgccc tgtccgcgcg cgcgcaggtc gaactcctgc
     7501 gtgtcggtgt ccacggttcc gctggcctcg accgcgccgg cctcgaagcg cgcgctcaag
     7561 cgttcgatgt tgaccttctt gccgccgaag gtgagacgcg cggccagctc ctcgtagcgt
     7621 tggctgccaa tcacgccggg cgcggcgcgc agctccgcgc tcccgctcat cgcgccgggt
     7681 agacccgtca cattcagttc tccagagacc ggccccatcg cggcgacgct ctgcgggttg
     7741 atgagcgggc cggcgtcggc gcttgtgcgc acgtcgccgc cgagggccgc gagcaggcgg
     7801 ccggcgttgg cgttctccag cgtcgcctcg accgcgatgt tgttctcgcc cgtgcgcgga
     7861 atcgtcgcac tgaagcgcgc gccgccgccg tccggctcga cgagctgacc atttttaacg
     7921 cgcgtctccg cggcgtcaga ctcgatgtcg gccgagagcg cgccgaggtc ctggccgcgc
     7981 gcgcgaagcg aggcgacctc gaagcggccg ttgacggtgg gcgcgtcgaa gtcaccgcgc
     8041 agcgtgccgt tgaatgaaag tttgccggcc agctcgacgc cgaagcgggc gagctgcgct
     8101 tcgacatcgg gcagcagccc cgtggagacg agcacgcgct ggaactcgga ggcgtcggcc
     8161 gagttgacgc ctacggcgag gtcggagccg cccgcaaacg agaagcgacc cgtggcggaa
     8221 agctcggtcg agccggcgcg caggttaccg cgctcgacct cgaagacgcc gcgctccgcg
     8281 ctcagcgcaa gctcgccgac gagcggcgtg cggcccgtcg cctcttgccc ggtctcgcct
     8341 gtgaacttcg cgttcagctt cccggaagcg gccttgtagt tcgtgccggg gaaactcaaa
     8401 tcgactgtgc ccgtggccgc gcccgtcagc ggcacgacgc tgcccgaggt ggccgcgacg
     8461 agtccgccca cgtccacgcc ctcgaacgcg gccgcgatgc gcgacgcgcc gccgcgcccc
     8521 gtcgcgaacg aggcgttgcc acgcgcgctg ccgccaaaga tttcggcgac gaagttttta
     8581 agctccgccg acgagttcgt cgccgtcacc tcgccgcgca gcgagccgag acgcatctcg
     8641 ccgagcacgc cgccgcctaa agagaggtcg gcgctcgcgc gatagcgccc gctcggctca
     8701 agctgaaaac gcggacgacc caagcgcacc tgctcggcgc gtccaccctg cgcgagcgtg
     8761 acggtgccgg cgtcgatgtc gcttgtcgtc ccctcaatca cgccgccggg cgagaccgac
     8821 aggcggacgc ccgcgatgtt gagaccgccc gtgcggtact gcgaggcagc gacgctgccc
     8881 agcgacacgc gcccgaccgt cacgtccgcc gactcgtccg gccgcacggt cgcgtcgatg
     8941 ttgctcgcgc ggatgtcgga gacgaccgtg tcctgatctt tgaccgatgc gaccgaagcc
     9001 gactcggccg tggcgcgaaa gcctccgccc tcgtcttgtc ggaagcgcac gccgccggcg
     9061 cgcacgccgc tcgcggccgt gtctccggtc gtcagcgtgc gcgccgagac accgccggcc
     9121 gtgccttcca gacggccctc tttaaactcc gcgacggcgt cgctcacgat gaggtcgccg
     9181 atgccggtct gcccaccgcg cacggccgcc gctctcaggt cgccgagcca gcggaagtca
     9241 gtgcccgtgc cgcgcacgcc gcccgcgagc tgcacgccgt tgatccgata gtcgcccgcc
     9301 gtcagaagct cggcgacggc cttgccctga acttcatacg tcttccccgt ccccgtgccc
     9361 gtcgcgttga cgttcaacgc ctgaagacgc acgccgtcgg cggccagcgc gtcggacgtg
     9421 agctgcccca cgaccttgta gttgtcgccc tcgccggaga cggcgccgtc gaagcggccc
     9481 gagccgcgca gggtcgtctc ggcgcggagc acgtccgagg tctgtgtgag atcgaccgtg
     9541 gcctcgacct tcatctggta gcggagcgcg cgccagtcgt cgagcgtccc cgtgagacgc
     9601 gcctcggcga cgggtgagcg caaaacgagt tcgctcactt cggcgcgcgt ctggttggcg
     9661 cggccccgcg cctcgatgtc gatgtcgttg acggggcgtc cgtcgtagac gaaggtcgaa
     9721 tccgacatcg cgatctcgaa gacgttcatc cggctctctt cgggcgcgtt ggggtcatcg
     9781 gggcgcacgg tggcgcgcag gttgcgcgcc tcgccggaga gcgagtgctg ccggtcgtcg
     9841 aagtggatga cggagctgtt gattttgatg ttggccgtcg agtaggagaa gaggatgcgg
     9901 ctgttggggt cgggcgcggg caggcgcagg ttggagaagt tcgagcgccc ctgctcgtcg
     9961 aagacgaccc acagtttcag gccgtcgagt tccatcgctt cgagcttcac gtcgcgcgtc
    10021 aggctgagcg catacatgtc ctcgatgcgg acggtggcga ggatgcgctc gatctcgccg
    10081 agcgcggcgc cggtctgcgc gtcgtagagc ttgacgtcct tgatctcggc cgtgcgcggc
    10141 ccgaacttgg tctcgaagga gccgatctcg gcgcggatgc cgtactcgct gaaggtctcg
    10201 acgatctggc gggcgatgat gcggtcaacc tgtcccgtgc ggtagaggat gacggcgacg
    10261 atgacgagga ggacgacggc cgcgacggcc gctatccccg tccacatcgc attgcggcgg
    10321 ttgacgacgc gccgccagcg ggcgcgacgg ccacgcggcg tcagcggcgc gggcgcgtcg
    10381 tcgccgcccg cggacgcctc cgtgaatttc acgggttcgc caccggccgg ctcggggccc
    10441 ttcgcgttgg gggcggagtc gtcgtgctcg tcaacgggca taaaacttca cgcggcggcc
    10501 gtccagggga agttaacggc gaacggactt gattaggaga ggatgaaccg aaacgatgaa
    10561 caatgactgc ggaatgatga attgaaacag gaagtggatg cctcttaatt catcactcat
    10621 cattccgcat tcagcatttg cttttccgcg cgcgccggcg agcctaaggg cacgctgccg
    10681 gcgatctcca gcccgaagcc ttcgagcgcc gagaccttcg gcgggtggtt cgtcaaaagc
    10741 acgatccgcc ttaagcccag ctcgtgcaga atctgcgcgc ccacgccgta gtcgcgctcc
    10801 acgccgtagc ccgtctccag acacgcctcc gtcttcgtca gcccgcgctc ctgcatgatc
    10861 tgataagtgc gtaactgatg gactaagtcg agattgttgt cgcgctggcg caggtagagg
    10921 acgaggccgc gcccctcggc ggcgatgcgt tcgagcgcgg cgtggagctg gtggcccgtg
    10981 tcgtccacga gcgtgccgaa catgtcgcag gtgacgttct cggagtggac gcggactaac
    11041 accggctcgt ccgttcgcac gtcgcccatg accatcgcga gatgcacgtc gccgttgacg
    11101 atgttctcga aggcgacggc gcggaagcgt ccgtaaacgg tcggcaggtc ggtctcgacg
    11161 gcgcggcgca ccagcacttc cttccgcatc cggtagcgca cgaggtcggc caccgagatg
    11221 atcttgaggc cgtgcttgcg cgcgaacttc ttcagctccg gcatccgcga catcgtcccg
    11281 tccgggttca taatttcgca gatgacgccg gccgggtaga gtccggcgat gcgcgccaga
    11341 tcgacgatgg cctcggtctg ccccgtgcgg acgagcacgc cccccgtgcg cgcgcgcagc
    11401 gggaagacgt ggccgggccg ggcgaggtct tgcgggcgcg tgcgcggatc gacggccgtc
    11461 agaatcgtgg tcgcgcggtc ggacgcggag atgcccgtgg tgacgccgcg ccgcgcctcg
    11521 acggagacgg taaaggccgt gccgagaaac gacgtgttgt cggccacctg catcgtcagt
    11581 tgcagctcgt cgcaacgctc ctcggtcagg ggcatacaga tgaggccgcg ggcgtgcgtc
    11641 gccatgaagt tgatcatctc gggcgtgacg cactcggcgg cgcaggcgag gtcgccctcg
    11701 ttttcgcgat cctcgtcatc gacgatgatg accatgcggc cgtcgctcaa gtcgcgcgcg
    11761 gcctcttcga tggaagcaaa aggcattttc gttgaacccg acaagctttt aggagagttg
    11821 tctgacagcg cgataatgtt acccgaaagt cgcgtttttg gcgacgtgaa aaagcggcgg
    11881 ccgcttccgt tcagagggcg accgaccgcg ccggggcccc tcacgggacg ctcaagtaag
    11941 tgacgaactc ctccggcgtg caaaccttgg gtatttcggc cgtgagcatc ccgtacttgt
    12001 cgatgggtat gaggaattcc tgcgcgggca ggcagagaac ccactcgacc cgcgtgcagc
    12061 cgaaggcccc gacgcccccg tcccggatta tctccgcgat ggtgacggcc gtctccgtcg
    12121 cctgcctgac tgccgcgccg atcctgccgc tgcgcgccgt gacggcgtcg taattcccca
    12181 gccagtattc cctggacttc gagtaggcct tgcactccac ggcgtagagc gtctcgccct
    12241 tcctgaccag caggtcaacc tcggccctct tgccgtcggc tgcctgaaag tatctcttcg
    12301 ccccgaccac ctcggcaccc gagccggact gctcaactag gcttttcagg ctgagcgtga
    12361 agcggtcgcc gtgctgcgaa ctgaaccact cacggccctt aacgaataac cagtggagga
    12421 agtcgggtat cagcagcaag tcgaagtagc agttgccgcc cggagaagtg taaaccagcg
    12481 ggcagggatc cagcgcgagc acgtcgatgc tatcccgcgt ctcctttgtc agcagaaacc
    12541 tatcgaagaa ggtattcacc agaagacttg cctgcgccgg cgagaacccg tccgggaagc
    12601 caactctatc cgacagcctc tcgcgtagaa ctgcctccgg cattcgcagg tatcccttct
    12661 gactgaggtc gaagaagaac ctgaccacgt ggtgggccgc cggatcgccc tccacgtcta
    12721 ggtcgcagat gatggccccg tccacggcct tcagcgcggg ggtcgtctgc cagacgcgac
    12781 tgctcagcga gcgcagcgtg ccgaaaatgg cctcgctgcc tgcgccgagg aagtcaacca
    12841 aagcctcctc gtaagcgcgc agactcgact ccagcgaagc ggtggcggtc gggtgcggga
    12901 ggtagtttct cagaaagagc gtaaaattgt ttttagggaa ggccaagggg atgggacgtc
    12961 ggagcctgtt cagcaaaatg atgttgtcgg cgccggcctg cacgggtgct ttgacgtgta
    13021 gtccctgatc ttcaagcagg ccgtccttcg gccggcgccg ctcgtagtgt ctgacggcct
    13081 caagcacggc gtcgtctgcc tcgaccactg ggaagccgtc cgcgccgacc aggacgacgg
    13141 cccccttgcc cgcccagcgg ttgagcccga cgatgttgtc gagcaaggat gaaaggttgg
    13201 cgattctgaa acaatcgaag aggtcgtcgt cggtgtaggc gagggccacg ccgaactggc
    13261 tttcggagtt gacctggcca aacgtcgagt cggtcgccca gtcggagtat ttaatgacgg
    13321 ccagcgtgca gtagatgacc caattgccgt cc
//
LOCUS       DASVES010000015         9640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1340999, whole genome shotgun sequence.
ACCESSION   DASVES010000015 DASVES010000000
VERSION     DASVES010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340999"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3072
                     /locus_tag="VF240_00640"
     CDS             <1..3072
                     /locus_tag="VF240_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7173776.1"
                     /translation="PHQPGDYRLLLDELRRDGNLPHQVLHLWNLSAADAAPTFDQSQS
                     VGYGSLLQLGKVLGEIDEPLEIVVVSNGLQCVTGEEELHPEKATLLGPCTTIPQEYPH
                     LTCRSIDVAMPLAGSWQEQKLCAQLVAESRAQDGEKVTAYRHGSRWVQAFEAVALDEP
                     AENSFGLREEGIYLITGGLGKIGLALAEYLTSAARVKVVLTGRSPLPARESWDERLKT
                     HGDDDETSRKILKLRALEESGAEVLYLCADVTDEEQMRAVFEVAEERLGPVCGVIHAA
                     GFLPEQTFRLIRDAQDSEGQWVNDPKVQGLLTLERVLSGRKIDFCVLMSSISSVLGGL
                     GYTTYAAANLFMDAFARKQNRLGPTPWVSVNWDAWTFGNEPQQQTGVGASLTAMSMTA
                     EEGLGIFRRLLAHGPFTQVVVSTTSLQDRIERWIKLDSLRAEKEQAAHAPRHPRPSLP
                     TSFVAPSSEAEKIIAEIWQQLLGLEQVGIHDDFFTLGGESLLAVQLTSRIRAALHVEI
                     GPGDLFDTPTVAALAEVVEGRRRHQPENNEAPPPRQGAVNEAAPLSFAQQQLWVVCQL
                     GDGETAYNIPTATLLRGPLNVGALEQSLNEIVRRHETLRTCFPVVDGRPLPVINPTLH
                     LSLPIIDLSETPAEGRLERARDIAGEEARTPFDLSCAPLLRARLVRAGEEEHVLLLTM
                     HHIVTDGWSIGVLIEEMAALYEAFSTGKPPSLPALPIQYSDFAAWQRRVLTGEKLESL
                     IDYWKQRLGDHLPKLKLPTDRPRSAARSLRAGTVPFLLAPPLPEALRALSREEGATLF
                     MTLLAAFDVLLRHHSGEDRVAVGTDVANRQRAEAEGMIGFFVNQLVLLTDLSGDPSFR
                     SLLARVREVTLGAYAHQEMPFGMLVELLNPEREVTHSPLFQVKLVLQNAPAPPVRLPN
                     LDLSPIEVENPYAKFDLLLTVWESERGLSCSFEYKEELYDRTTIVDMISDYEAIVLAV
                     VSEPNARLSTLDELLAERRRRRVLAREREAQEVARMKLKNVKRKPLGPAGT"
     gene            3148..>9640
                     /locus_tag="VF240_00645"
     CDS             3148..>9640
                     /locus_tag="VF240_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX7173777.1"
                     /translation="MHDSPIEGFRLSPQQRRLWKLQEAEGADPFPALCAVSVEGVIVP
                     ETFRDAVEQTVRRHEILRTTFHLLPRMDAPIQAVGEGRFDFTQIPAPKERPPGGEQAL
                     IDTLVEEAARLPFDLKNGPVLRLTLHALSDSTHLLIVTLPALCADVLTLTKLVGEIAK
                     AYTALLSGEQHAAETIQYADVSEWQNELLEAEDAHAGKDYWRRQELHTARPLSLFDEH
                     PGGEQAVYDPRRFRVMLPASVMAEVKALAFEYTCSPQAVVLSCWQAVLWRLTGESEVV
                     VSAAFKGRQYAELEDGLGLFAKYLPVRALLKEDLTFADLTKAVEASLDESGRWQEYFI
                     EDGGTRSEGKTGTLGVGFEFIEWPRPINAGGLSFSFRRLYSHFDRFKVKLTCHQTDDA
                     LLVEFQYDGALLRQQTIERLAANLQTLLTDALARPRAAIQSLDILGSDERRALLYEFN
                     ATATAQPTSSCFHTVFERQVEETPDAAAVSVGRTTYSYRELNERANKLARRLREAGVG
                     PEVLVGILLSRSVEMVVGLLGVLKAGGAYVPFDISQPARRLGFMLEDAGVTLLLCHES
                     TSDTELPPHLKLMRLDADWAEIESYSAGNLPPSALPDNLAYVIYTSGSTGRPKGVQVT
                     HASLVNLLSSMQRIPGLTSSDTLLAVTTISFDIAALELFLPLVTGARLVIATRDEAHD
                     AGRLAGLLSDFSVTVMQATPATWRLLVESGWAGSAGLKILCGGEALARSLAEELVGRG
                     AAVWNLYGPTETTIWSAAGRVDGGTGEGLVSVGGPIANTQMYVLDAELEPAPVGVVGE
                     LYIGGEGLARGYLNRPELTAERFVPHPHGEEAGARLYRTGDVARYRSDGGMEVVGRMD
                     QQVKLRGHRIELGEVESALCGHPEVREAAVVVRERRPGDQRLVAYLVSGRDARPAAAE
                     LSGFVRRSLPEFMAPSSFVYLDALPRHPNGKVNRPALPEYDDTTAEAGASGGPPQTQL
                     EGAIADIWKDVLGVAKVSLRDNFFDLGGHSLLLAQVHRRLREVVAKDISLIEMFRHPT
                     VAALAGYLNNGHNSHSALASSAVPEPAVRRSEETGAGTEVAIIGMTGRFPGAKTVDEF
                     WRNLRDGVESITFFSDEELAAEGCDPALLRDPKFIRAGATLDGVELFDAKFFGFSPRE
                     AEVTDPQHRLFLECAWEAMENAGYDPARFGAGVGVYASAGMNTYLLFNLAGRPDLLTP
                     QSAYQTFIGNDKDFLTTRVSYKLNLKGPSVNVQTACSSSLVAVHLACQSLLNRECDMA
                     LAGGVAVKVPAKAGYLHDEQGIDSPDGHCRAFDAKAAGTVFGSGVGVVVLKRLADALS
                     DGDYIHAVIKGSAVNNDGSNKISYTAPSEAGQAEVISMALAAAGVEPETIGYVETHGT
                     GTHLGDPIEIAALTKAFGSGAQKKGYCAVGSVKTNIGHLDTAAGVAGLIKAVLALKNR
                     ELPPLLHFEQPNPQIDFAGGPFYVNEQTRAWEQGPGPRRAGVSSFGIGGTNAHVILEE
                     APAPATAGAARPWQLLTVSAKTEPALELAADALAAHLKNDPDASLPDVAYTLHVGRQS
                     FEHRRLVVCRDAREAVASIERMEAKRVFTGHEESASRPVAFLFPGQGTQHVGMARELY
                     QTEPSFRRTVDECSELLRPRLGLDLRAILYPSDDAAAAERQLEQTRLAQPALFVVEYA
                     LARLWEEWGVRPRAMLGHSIGEYVAACLAGVFSLENALALVAARGRLMQSMPAGAMLA
                     ASLAEDEARALLNEQLSLAAVNGASQCVFSGETQAVERLEAELNRRGLACRRLHTSHA
                     FHSVMMEPVAERLADELARVKLEPPRRAYLSNLTGDWITPREATDPNYWVRQLRETVR
                     FSDGLGKLLETPEQILLEVGPGQSLATQTRRHPKRHPQQTTVSSLRHPHDSDSSDAAL
                     LLTALGQIWLAGVPVDWPAYHTHERRRRVPLPTYPFERQRYWVDPVAREDGGAHESLR
                     KRPNLSEWFYLPCWKPAAPARSSEAPREHARRLVLINKNSFCTALLRSLEEQSDDTLV
                     TVQAGEHFAKHSGGAYVVNPHQPGDYRLLLDELRRDGNLPHQVLHLWNLSAADGAPTF
                     DQSQSVGFGSLLQLGKVLGEIDEPLEIVVVSNGLQCVTGEEELHPEKATLLGPCKTIP
                     QEYPHLTCRSIDVAMPLAGSWQEQKLCAQLVA"
BASE COUNT         1729 a         3034 c         3206 g         1671 t
ORIGIN      
        1 ccgcatcagc ccggcgacta tcgactgttg ctcgacgaac tccgacgcga cggcaatctc
       61 ccgcatcaag ttttgcacct gtggaatctc tcggcggccg acgcggcgcc gaccttcgac
      121 caatctcaaa gcgtgggtta cgggagtctg ctccaactgg ggaaggttct gggagagatt
      181 gacgagccgc tggaaatcgt cgtcgtctcc aacggccttc aatgtgtcac cggcgaagag
      241 gaactgcacc cggagaaggc gacgctgctc ggcccctgca caaccatccc gcaggagtat
      301 ccgcacctca cctgccgaag tatcgatgtc gccatgccac ttgcggggag ttggcaggaa
      361 caaaagctgt gcgcgcaact cgtcgccgag tcgagggcgc aagatgggga aaaggtcact
      421 gcgtatcgcc acggctcgcg gtgggtgcaa gccttcgagg ccgtggcgct cgacgagccg
      481 gccgaaaaca gctttggctt gagggaggaa gggatttacc tgataaccgg cggtctgggc
      541 aagatcgggc ttgcgctggc ggagtatctg acgagcgcgg cgcgtgtgaa agttgtgctc
      601 accggccgct cgcctctgcc cgcgagggag agctgggacg aacggctgaa gacacacggc
      661 gacgacgacg aaacaagtcg aaaaatcctg aagctgcgcg ccctcgaaga gtcgggggca
      721 gaggtgctct acctctgcgc cgacgtgacc gacgaggaac agatgcgcgc cgtcttcgag
      781 gtggcggagg aacgattggg gccggtctgc ggcgtcatcc acgcagcggg cttcctcccc
      841 gaacagacgt ttcgcctcat cagagacgcg caggactccg aaggtcagtg ggtcaacgac
      901 ccgaaggtgc aggggctgct cacgctggag cgagtcttga gtggacgaaa gatagatttt
      961 tgcgtgctca tgtcttcaat ctcctccgtg ctcggaggtc tcggctacac gacctacgcg
     1021 gcggcgaatc tcttcatgga tgcgttcgcc cgcaagcaga accgactcgg cccgaccccg
     1081 tgggtcagcg tcaactggga cgcatggacg ttcgggaacg agccgcaaca gcagacgggc
     1141 gtgggggcga gcctgacggc gatgtcgatg accgcggagg agggcttggg aatcttccgc
     1201 cgcctgttgg cgcacgggcc gttcacacag gtcgtcgtct cgacgacgag cttacaggac
     1261 aggatagaac gctggatcaa acttgattct ttgcgtgccg agaaggagca ggccgcgcac
     1321 gcgccgcggc acccaagacc gtctttgccg acgagcttcg tggctccgag cagcgaggcg
     1381 gaaaaaatca ttgccgaaat ctggcaacaa ctgctggggc tggaacaagt cggcattcac
     1441 gacgactttt tcaccctcgg cggcgagtcg ctgctggccg tccagttgac ctcgcgcatc
     1501 cgcgcggctc tccatgtgga gatcgggccg ggtgacctct tcgacacgcc cacggtcgcg
     1561 gcgttggcgg aagtcgtcga gggtagacgc cgccatcagc ccgagaacaa cgaagcgccg
     1621 ccgccgcggc agggtgcggt gaatgaagcc gcgccactct ctttcgcaca acagcagttg
     1681 tgggtcgtgt gccagttggg cgacggcgag acggcctaca acatcccgac cgccactctc
     1741 ctgcgcggcc ccctcaacgt cggcgctctc gaacagagtc ttaacgagat cgtgcggcgg
     1801 cacgagaccc tacgcacttg tttccccgtc gtggatggca ggccgctgcc ggtcatcaat
     1861 cccaccctgc acctgagcct gccaatcatc gacctcagcg aaacgcccgc ggaggggcgt
     1921 ctggagcggg cgcgcgacat cgcgggcgag gaggcgcgaa cgccattcga tctgtcgtgc
     1981 gctccgctgc ttcgcgcgcg gctcgtgcgc gcgggcgagg aggagcacgt gctcctgctg
     2041 acgatgcatc acatcgtcac cgacggctgg tcgataggcg tgctcatcga agaaatggcc
     2101 gccctctatg aagccttctc caccggcaag cctccttcgc tacccgcact cccgattcaa
     2161 tactccgact tcgcggcctg gcagcgtcgc gtgctcacgg gggaaaagct tgagtcgttg
     2221 atcgactact ggaagcaacg tctcggggat cacctgccga agctgaaatt gccgaccgac
     2281 aggccgcgct ccgcggcccg gagtctgaga gccgggacgg ttccgttcct gctcgcgccg
     2341 ccgctaccgg aagcgctgcg tgcgttgagc cgggaagagg gcgcgaccct gtttatgact
     2401 ctcctcgcgg ccttcgacgt gctgctccgt caccactcgg gcgaggatcg agttgcggtg
     2461 gggacagacg tggccaaccg gcagcgggcg gaggccgaag ggatgatcgg cttcttcgtc
     2521 aaccagctcg tgctgctcac ggatttgtcc ggcgacccga gttttagatc gctgctggcg
     2581 cgcgtgcgcg aagtgacgct cggcgcatac gcccatcagg aaatgccgtt cgggatgctc
     2641 gtcgagttgt tgaacccgga aagggaagtc acacactctc cgctctttca ggtgaagcta
     2701 gtccttcaga acgcgcccgc cccgccggtg cgtctgccga atctcgacct cagccccatc
     2761 gaggtcgaga acccttacgc gaagttcgat ctgctgctca ccgtctggga aagcgagcgc
     2821 ggactgtcct gttcgttcga gtacaaggag gagttgtatg accgcaccac gattgtagac
     2881 atgatcagtg attacgaagc catcgtcctc gccgttgtgt cggagccaaa cgcacgcctc
     2941 agcacccttg acgagctgtt ggccgagcgg cgtcggcgac gtgttctcgc gcgcgagcga
     3001 gaggcgcagg aagttgcgcg gatgaagtta aagaacgtga agcggaagcc gctcggcccc
     3061 gccggcacat aggcgccaaa aggagcgcac tgaccacggg ctgccaccca ttgcgcttgg
     3121 gtcttactac cagcaggaaa cacgaacatg cacgactctc ccatcgaagg ttttcgtctt
     3181 tcgcctcaac aacgtcggct ctggaagctg caagaggcgg agggcgcaga cccattcccg
     3241 gcgctgtgcg cggtgtcggt cgagggcgtg atcgtgccgg aaacttttcg ggacgccgtc
     3301 gagcagacgg tgcggcggca tgaaattcta cgcaccactt tccacctgtt gccgcggatg
     3361 gacgccccaa tccaggctgt cggcgagggc cgcttcgact tcacacaaat ccccgccccg
     3421 aaagagcgcc cgcccggcgg cgagcaggcg ctgattgata ctctcgtcga agaggccgcg
     3481 cgccttccct tcgatctgaa gaacggcccc gtcctgcgtc tcacgctgca tgcgctttcc
     3541 gattcgaccc accttctgat cgtcacactg cccgcactct gcgcggacgt gttgacgctg
     3601 acgaagctgg tgggagaaat cgcgaaagcg tatacggccc tgctgagcgg cgagcagcat
     3661 gccgcggaga caattcaata cgcggatgtc tccgagtggc aaaacgagtt gcttgaggcg
     3721 gaggacgcgc acgccgggaa agattactgg cgcaggcagg agttgcacac ggcccggccc
     3781 ctgagtctct tcgacgaaca tcccggcggc gagcaggctg tttatgatcc acgtcgtttc
     3841 cgagtgatgt tgcccgcgtc cgtcatggcc gaggtgaagg cgctcgcttt cgaatacacg
     3901 tgctcgcctc aagcggtcgt gctctcttgc tggcaggcgg tgctgtggcg actcacgggg
     3961 gagtccgagg tcgtcgtcag cgcggctttc aaagggcgac agtacgcgga attggaggat
     4021 ggtctcggcc tgttcgcaaa atatctaccc gtccgcgctc tcttgaagga ggatttgacg
     4081 ttcgccgatt tgacgaaagc cgtcgaggcg agtttggacg agagcggccg gtggcaggag
     4141 tattttatcg aggacggggg cacgcgctcg gaggggaaga cgggcacgct gggggtcggc
     4201 ttcgagttta tcgaatggcc gcgcccgata aatgccggcg ggctgtcgtt ctcctttcgc
     4261 cggctttact cccacttcga ccgctttaaa gtaaaactaa cttgtcatca aacggatgac
     4321 gcgctcctcg tcgagtttca gtatgacggc gcgttattgc ggcagcagac aattgagcgt
     4381 ctggccgcga acctgcaaac gctgctgacc gacgccctcg cccgtccgcg cgcggccatc
     4441 cagagtctcg acattctggg cagtgacgaa cgtcgggcgc tgctctacga attcaacgcc
     4501 acggccacgg cccagcccac ctcgtcgtgc ttccacacgg tcttcgagcg tcaggtcgaa
     4561 gagacgccgg acgcggcggc cgtcagcgtt gggcgcacga catacagcta tcgcgagttg
     4621 aacgagcggg ccaacaagct ggcgcggcgg ctgagagagg cgggcgtcgg gccggaagtg
     4681 ttggtcggca tcctgttgag tcgttcggtt gagatggtcg tgggtctgct cggcgtgctc
     4741 aaggccggcg gcgcatacgt cccctttgac atctctcaac cggcgcggcg gctcggcttc
     4801 atgctcgaag acgcgggcgt gacgttgctc ttgtgccatg agtccacgtc cgatacggag
     4861 ttgccgccgc atctgaagct catgcgcctc gacgcggact gggcggagat cgagagctac
     4921 agcgccggca accttccgcc ttcggccctg cccgacaatc tcgcctacgt gatttacact
     4981 tccggttcga cgggccggcc caagggcgtg caggtcaccc acgcctccct cgtcaacctg
     5041 ctctcttcca tgcagcgcat ccccggtctg acctcttcgg acacgctgct cgccgtcacc
     5101 accatctcct tcgacatcgc cgcgctggag ttgttcctgc cgctcgtgac gggcgcgcgc
     5161 ctcgtcatcg ccacccgtga cgaggcccac gacgcggggc ggctggcggg gcttctgtcg
     5221 gacttctccg tgaccgtgat gcaggcgacg ccggcgacgt ggaggctgct cgtcgagtcg
     5281 gggtgggcgg ggagcgcggg gttgaagatt ctgtgtgggg gcgaggcgtt ggcgcgctcg
     5341 ctggccgagg agttggtggg gcgcggcgcg gcggtgtgga acctctacgg gccgacggag
     5401 acgaccatct ggtcggcggc cggaagggtc gatgggggaa cgggtgaggg gttggtgagc
     5461 gttggcgggc cgatagccaa cacgcagatg tacgtgctcg acgctgagtt ggagccggcg
     5521 ccggtggggg tggtggggga gttgtacatt gggggagagg ggctggcgcg cgggtatctg
     5581 aaccggccgg agctgacggc ggagaggttc gtgccacacc cgcacgggga ggaggcgggg
     5641 gcgaggctgt accggacggg ggacgtggcg cgctaccgct cggacggcgg gatggaagtg
     5701 gtggggcgga tggatcagca ggtgaagctg cgcggccatc gcatcgagtt gggcgaggtt
     5761 gagtcagctt tgtgcggcca cccggaagtg cgcgaggcgg ccgtcgtcgt tcgcgagagg
     5821 cgtccgggcg atcagcgtct ggtcgcgtat ctggtgagcg ggcgagacgc tcgccccgcc
     5881 gctgcggagt tgtccggctt cgtgcgccgg agtctgccgg agttcatggc gccttcgagc
     5941 ttcgtctacc tcgacgcctt gccgcgtcac ccgaacggca aagtcaaccg ccccgccctg
     6001 cccgaatacg acgacacgac ggcggaagca ggggcctcag gcgggccgcc gcagacgcag
     6061 ttggaagggg ccatcgccga catctggaaa gacgtgctgg gggtcgcgaa ggtgagtttg
     6121 cgcgacaact tttttgacct cggaggacac tcgctgctcc tcgcacaggt gcatcgccgg
     6181 ctgcgcgagg tcgtcgcgaa agacatctct ctgatcgaga tgttcagaca cccgaccgtc
     6241 gccgcgttgg cgggctattt gaacaacggg cacaactctc actcggcgct cgcatcatca
     6301 gccgtacccg aacccgccgt gcgcaggagc gaggagacag gcgcgggcac ggaggtcgcc
     6361 atcatcggga tgaccgggcg cttccccggt gcgaaaacag tcgatgagtt ctggcgtaac
     6421 ctgcgtgatg gggtcgagtc gatcacgttc ttcagcgatg aggagcttgc ggcggaaggg
     6481 tgcgatcccg cactgttgcg cgacccgaag ttcatccggg cgggggcgac gctcgacggc
     6541 gtcgaactgt tcgacgcgaa gtttttcggc ttcagcccgc gggaggccga agtcaccgac
     6601 ccgcagcacc gcctgttcct cgaatgcgcg tgggaggcga tggagaacgc gggttacgac
     6661 cccgcgaggt tcggcgcagg ggtcggcgtc tacgccagcg ccgggatgaa cacgtacctg
     6721 ctcttcaacc tcgcgggccg ccccgacctt cttacgccac agtccgccta tcagaccttc
     6781 atcggaaacg ataaggactt ccttacgacg cgtgtctctt acaagctgaa cctgaaaggg
     6841 ccgagcgtca acgtccagac cgcgtgctcc agctcgctgg tcgccgtcca tctcgcctgc
     6901 cagagcttgt tgaaccggga gtgtgacatg gcgctggcgg gcggggtggc cgtcaaggtg
     6961 cccgcgaagg ccggctatct gcacgacgag cagggaatcg attcgcccga cgggcattgc
     7021 cgtgctttcg acgcgaaggc cgccgggacg gtgttcggca gcggcgtggg cgtcgtcgtt
     7081 ctgaagcggc ttgcggacgc cttgagtgac ggcgattaca tccacgccgt catcaaaggc
     7141 agcgccgtca ataacgacgg ctcgaataaa atcagctata ccgcgccgag cgaagccggc
     7201 caagccgagg tcatctcgat ggccctcgcg gccgccgggg tcgagccgga aaccatcggt
     7261 tacgtcgaga cacacggcac cggcacacac ctcggcgacc ccatcgaaat cgccgccctg
     7321 acgaaagcct tcggcagtgg cgcacagaaa aagggatact gcgccgtcgg gtcggtcaag
     7381 acaaacatcg gccatctgga cacggccgca ggcgtcgccg gattgattaa ggccgtgctc
     7441 gcgttgaaaa accgggagct gccaccgcta ctacacttcg agcagccgaa cccgcagatc
     7501 gatttcgcgg gcggcccctt ctacgtcaac gaacagacgc gcgcgtggga gcaaggcccc
     7561 ggcccgcgca gggccggcgt gagttccttc ggcatcggcg gcacgaacgc ccacgtcatt
     7621 ctcgaagagg ctccggcccc ggccacggcg ggggcggcga ggccgtggca gttgttgact
     7681 gtctcggcga agaccgagcc ggcgctggaa ttagccgccg acgccctcgc cgcacattta
     7741 aaaaatgacc cggacgccag tctgccagac gtggcctaca ccttacacgt gggccgccag
     7801 tcgttcgagc atcgtcgcct cgtcgtctgc cgtgatgcgc gcgaggccgt cgcttccatc
     7861 gagcgcatgg aggcgaagcg ggtcttcacg ggacacgagg aatcggcttc gcgcccggtg
     7921 gccttcctct tccccggaca gggcacgcag cacgtcggga tggcgcgcga gctttatcaa
     7981 actgagccga gcttccggcg gacggtggat gagtgctcgg agttgctgag gccgcgcctc
     8041 ggtctcgacc tgcgcgcgat cctctacccg tcagacgacg cggcggcggc ggagcgtcaa
     8101 ctcgaacaga cacggctggc acagccggcg ctcttcgtcg tcgagtatgc ccttgcgcgt
     8161 ttgtgggagg agtggggcgt gcggccccgc gcgatgctcg gccacagcat cggcgaatac
     8221 gttgcggcct gcctcgctgg cgtcttctcg ctcgagaacg ccctggcgct ggtcgcggcc
     8281 cgagggaggc tgatgcagag catgccggcc ggggcgatgc tggccgcctc gctcgcggaa
     8341 gacgaagcgc gcgctctcct caacgaacaa ctctcgctgg cggccgtcaa tggagcgtcg
     8401 caatgcgtct tctccggtga gactcaggcc gtcgagcggc tggaggccga actaaaccga
     8461 cgcggtcttg cgtgcaggcg acttcacacg tcgcacgcct ttcactccgt gatgatggag
     8521 ccggttgcgg agcgtcttgc cgatgagctg gcgcgtgtga agctggaacc gccgcgtcgc
     8581 gcatatctct caaacctgac gggtgactgg atcacgccgc gtgaggcgac cgacccgaac
     8641 tactgggtca gacaactgcg cgagacggtg cgcttctccg acggcctcgg taaattactt
     8701 gaaacgcccg aacaaatcct gctcgaagtc ggccccggtc agagcctcgc gacgcagacc
     8761 cgcaggcacc cgaagcgaca cccgcagcag accacggtgt cgtcgttgcg tcacccgcac
     8821 gactccgatt catcggacgc ggcgctgttg ctcaccgcgc tcggacaaat ctggctggcg
     8881 ggcgtgccgg tggattggcc cgcctaccac acccacgaac gacggcggcg cgtcccgttg
     8941 ccgacttacc ccttcgagcg tcagcgttac tgggttgatc ccgtggcgcg cgaagacggc
     9001 ggtgctcatg agtcgctgcg gaagagaccg aacctctccg agtggttcta ccttccgtgc
     9061 tggaaacccg ccgcgcccgc gcgcagctcc gaggcgccgc gtgagcacgc ccgccgcctc
     9121 gtcctcatca acaagaactc gttctgcacg gcgctgctca gaagcctcga agagcaatcc
     9181 gacgataccc tcgtcaccgt gcaggcaggc gagcacttcg cgaagcacag cgggggagca
     9241 tacgtggtga acccgcatca gccgggcgac tatcgactgt tgctcgacga actccggcgc
     9301 gacggcaatc tcccgcatca ggttctgcac ctgtggaatc tctccgcggc cgatggggcg
     9361 ccgaccttcg accaatctca aagcgtgggc ttcgggagtc tgctccaact ggggaaggtt
     9421 ctgggagaga ttgacgagcc gctggaaatc gtcgtcgtct ccaacggcct tcagtgcgtc
     9481 accggcgaag aggaactgca cccggagaag gcgacgctgc tcggcccttg caaaaccatc
     9541 ccgcaggagt atccgcacct cacctgccga agtatcgatg tcgccatgcc acttgcgggg
     9601 agttggcagg aacaaaagct gtgcgcgcaa ctcgtcgccg
//
LOCUS       DASVES010000016         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1729089, whole genome shotgun sequence.
ACCESSION   DASVES010000016 DASVES010000000
VERSION     DASVES010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729089"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..921
                     /locus_tag="VF240_00650"
     CDS             70..921
                     /locus_tag="VF240_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7173778.1"
                     /translation="MKALLIVLLLTGSAAAQELPSRWDELTAADWPKAQERAAYTCVL
                     PIGILEKHGPHMPMGSDLIQVRELSARAAKQEYAVVFPDFFYGQIYEAQHQPGTFALP
                     SRVVWDLLDATVEEIARNGFKKIVIVNGHGGNPQLLRYFVQAQLERRRDYVVYFFEPE
                     WDAEFDQKQKALRKSDPATDMHAGENETSALLYLRPDLVQMNTAAQESGDDQKRLSLP
                     GLYTAIWWYARFPNHYAGEGAKATRELGQALAEHRISALVKALRAVKADDKTPAIQKE
                     YFDKIKK"
     gene            complement(1072..2760)
                     /locus_tag="VF240_00655"
     CDS             complement(1072..2760)
                     /locus_tag="VF240_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX7173779.1"
                     /translation="MKDKILISALAVVFILGLSPGARAQSTNAGGRAAVTFAEVPARA
                     SGISWVHNNAHSRERFLPETVGAGGAFFDYDNDGWLDIYLVNSGPADFYTPAVPLKNA
                     LYRNNRDGTFTDVTERAGVAGGRFGMGAAAGDFDGDGRADLYVANYGPDILYRNNGDG
                     TFSNVTEKAGIDVPGWSTCAVWFDYDADGRLDLFVSSFVHFDKSQHRLCVDKTATQNY
                     YCIPRIFKPRPSRLFRNNGDGTFSDVSKESGIADSPGKSFGAVATDVNNDGLMDLFVA
                     NDTMPNFLFVNRGRGKFEEAGLGAGVAYGEAGTPRSGMGVDAGDYDGDGWQDLFVANI
                     DQEFFSLYHNQQDLTFTDEPGEIAPVTQLLSGWGLKFFDYDNDGDPDLLLANGHPDDM
                     VETKSARVKYKEPLLMFENTGKGYRNVSAQSGAVFARSFPARGMAVGDYDNDGDLDAI
                     VFNNGEAPLLLRNEGGSRHHWLGLQLVSTKSNPAAVGAHITWQAGGVRRSRLKTSGGS
                     YLASHDPREILGLGAATKIDALEIRWPSGAVDKLSNLPAGAYLRVVEGQGVVKK"
     gene            complement(2775..>3821)
                     /locus_tag="VF240_00660"
     CDS             complement(2775..>3821)
                     /locus_tag="VF240_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7173780.1"
                     /translation="IGGGQLAEAERQLNQILRSAPNEAAALNLLGALRAKQGRLDEAE
                     ALFSRSVRGDNRLTGAYMNLAYLYLLKREPEKSAASLREVLRLEPSNADAAHRLAWLL
                     HSQGRFDECIGFVEKLKQSQTLTAPVLALLADAYLKKGSAGEAVSALKKAIELQPGEP
                     SYHFALGAAWLRQPPDLLGAEQSFRQFLTLRPDDAPGQLHLGYVLLKQKRHAEARVWL
                     ERSTGKGAGTPEAFYYLGLIAQGENEDARAIELFEKSIQLAPSFAHVHVALGSTYMKL
                     KDYPRAQQALEAGVKLSPEDTKAHYNLAMLFARLKNPERAQEEMRIVARLKNEGKARP
                     EDDAGEDGAPPPPR"
BASE COUNT          628 a         1248 c         1253 g          692 t
ORIGIN      
        1 actcaagaga atgccgctct aaacttcccc tccgcggctt ccgcttcgac caattcacgg
       61 agactcacaa tgaaagcact gctgatcgtt ctcctgctca ccggcagcgc cgccgcgcag
      121 gagttgccct cgcgctggga cgagttgacc gcggccgact ggccgaaggc gcaggagcgc
      181 gccgcctaca cctgcgtcct ccctatcggc atcctcgaaa agcacggccc gcacatgccc
      241 atgggttccg acctcattca agtccgcgag ctatcggcgc gcgccgcgaa gcaggagtac
      301 gccgtcgtct tccccgactt cttctacggc cagatttacg aggcgcaaca ccagccgggc
      361 actttcgcgc tgccgtcgcg cgtcgtgtgg gacttgctcg acgccacggt cgaagagatc
      421 gcccgcaacg gcttcaagaa gatcgtcatc gtcaacggcc acggcggcaa cccgcaactg
      481 ctgcgctact tcgttcaggc gcagctcgaa cgccgccgcg attacgtcgt ctacttcttc
      541 gagccggagt gggacgcgga gttcgaccag aagcagaagg cgctgcggaa gtccgacccg
      601 gccacggaca tgcacgccgg cgagaatgag acctccgcgc tcctctacct gcgccccgac
      661 ctcgtccaaa tgaacaccgc cgcgcaggag tccggcgacg accagaagcg cctgtctctg
      721 cccggtctct acacggccat ctggtggtac gcgcgcttcc ccaaccacta cgccggcgag
      781 ggcgcgaagg ccacgcgcga actcggacag gcgctcgccg aacaccgcat cagcgccctc
      841 gtcaaagccc tgcgcgccgt caaagccgac gacaagacac ccgcgattca gaaggaatac
      901 ttcgacaaga tcaagaagtg aagaagtgat gagtgaagaa gtgatgagtg ataagtgggc
      961 tgttagccgt tagccgttag ctgtcagccg gacaggctca aagccggcgg cggcggtatg
     1021 ccagaggcta atggctaaga gctaagagct aaccgccctt cttatcactc atcacttctt
     1081 cacaacgccc tgcccttcga ccactctgag gtaggcgccg gcggggaggt tggagagttt
     1141 gtccacggcg ccgctgggcc agcgtatttc gagggcgtcg attttcgtgg ccgcgccgag
     1201 gccgaggatt tcgcgcgggt cgtgtgaggc gaggtaactg ccgccgctcg ttttgagacg
     1261 gctccggcgc acgccgcccg cctgccaggt gatgtgtgcg ccgacggcgg ccgggttgct
     1321 cttcgtcgag acgagttgga ggccgagcca gtggtgacga ctgccgccct cgttacgcag
     1381 caggaggggg gcctcgccgt tgttgaaaac gatggcgtcg aggtcgccgt cgttgtcgta
     1441 gtcgccgacg gccatcccgc gggccgggaa gcttctggcg aagaccgcgc ccgactgcgc
     1501 gctcacgttc cggtagccct tccccgtgtt ctcgaacatg aggagcggct ctttgtactt
     1561 cacgcgcgcc gacttcgtct cgaccatgtc gtccgggtgg ccgttggcga gaagcagatc
     1621 ggggtcgccg tcgttgtcgt agtcgaagaa cttcagcccc cagccgctca ggagctgcgt
     1681 gacgggcgcg atctcgcccg gctcgtcggt gaaggtcaaa tcctgctggt tgtggtagag
     1741 gctgaagaac tcctgatcga tgttggcgac gaagaggtct tgccacccgt cgccgtcgta
     1801 atcgccggcg tccaccccca tccccgagcg cggcgtcccg gcctcgccgt aggccacgcc
     1861 cgcccccagt cccgcctcct cgaacttgcc tcggccgcgg ttgacgaaga ggaagttggg
     1921 catcgtgtcg ttggcgacga acagatccat cagcccatcg ttgttcacgt ccgtcgccac
     1981 cgcgccgaac gacttccccg gcgagtccgc gatgccggat tctttgctca cgtcactgaa
     2041 cgtcccgtcg ccgttgttgc ggaaaaggcg gctcggccgc ggcttgaaga tgcgcgggat
     2101 gcagtagtag ttctgcgtcg ccgtcttatc gacgcagagg cggtgctgcg acttgtcgaa
     2161 gtgaacgaag ctggagacaa agaggtcgag gcggccgtcg gcgtcgtagt cgaaccagac
     2221 ggcgcaggtt gaccagcccg gcacgtcgat gccggccttc tccgtcacat tactgaacgt
     2281 gccgtcgccg ttattgcgat acaggatgtc cggcccgtag ttggcgacgt agaggtctgc
     2341 gcgtccgtcg ccgtcgaagt cgccggcggc cgcgcccatc ccgaaccggc cgccggccac
     2401 gccggccctc tccgtcacgt cggtgaaagt cccgtcgcgg ttgttgcggt agagagcgtt
     2461 tttgagcggg acggccggcg tgtagaaatc cgccgggccg ctgttgacga gatagatgtc
     2521 gagccacccg tcgttgtcgt agtcgaagaa ggcgccgccg gccccgaccg tctccggcag
     2581 gaagcgttcg cgcgagtggg cgttgttgtg aacccaactg atgccgctcg cccgggccgg
     2641 cacctcggcg aacgtgacgg ccgccctgcc gcccgcgttc gtgctctgcg cgcgcgcgcc
     2701 gggcgacagg cccagaatga acacgacggc cagcgccgag atgagaatct tgtctttcac
     2761 aaacgttgtc cgcactagcg gggcggcggg ggcgcgccgt cttcgcccgc gtcgtcttcc
     2821 ggccgcgcct tgccttcgtt cttcagtctg gcgacgatac gcatctcctc ctgcgcccgc
     2881 tccgggtttt tgagccgggc gaagagcatg gcgagattgt agtgcgcctt cgtgtcttcg
     2941 gggctgagtt tcaccccggc ttcgagcgcc tgctgcgcgc gcgggtaatc tttcagcttc
     3001 atgtaggtcg agccgagcgc gacgtgaacg tgggcgaacg agggcgccag ttggatggac
     3061 ttctcgaaga gttcaatggc gcgcgcgtcc tcgttctcgc cctgcgcgat cagacccaga
     3121 tagtagaagg cttcgggcgt gcccgcgccc ttgcccgtgc tccgctccag ccacacgcgg
     3181 gcttcggcgt gccgcttctg cttgagcagc acgtagccga ggtggagttg ccccggcgcg
     3241 tcgtcgggcc ggagcgtgag gaactgtcgg aaagattgtt cggccccgag aaggtctggc
     3301 ggctggcgga gccatgcggc gccgagggcg aagtggtatg acggctcgcc gggctggagt
     3361 tcgatggctt ttttaagcgc cgacacggcc tcgcccgcag agcctttttt cagatacgcg
     3421 tcggcgagta aggcgagcac gggggccgtg agcgtttgcg actgcttcag cttttcgacg
     3481 aagccgatgc actcgtcgaa gcggccctgc gagtgcaaca gccaggcgag ccggtgggcg
     3541 gcgtcggcgt tggacggttc gaggcgcagc acttctcgca acgacgcggc cgacttctcc
     3601 ggctccctct tgagcagata gagataggcc agattcatgt acgcgcccgt caggcggttg
     3661 tcgccgcgga cggagcgcga gaacagggct tcggcctcgt cgagtctgcc ctgcttggcg
     3721 cggagcgcgc cgagcaggtt gagcgccgcc gcctcgttcg gcgcggagcg caggatttga
     3781 ttcaactgcc gctcggcctc cgcaagttgg ccgccgccga t
//
LOCUS       DASVES010000017        14282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_951269, whole genome shotgun sequence.
ACCESSION   DASVES010000017 DASVES010000000
VERSION     DASVES010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14282
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951269"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VF240_00665"
     CDS             <1..598
                     /locus_tag="VF240_00665"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7173781.1"
                     /translation="TAGKARNQAGHQPRQARQTAKRFWTETRIAVAVTALAVTAVLGL
                     ATCGPSKTQRGPNPVLDTDLQLVDGGTFRLAEYDGRVLVLNFWATWCGPCRSEIPHLV
                     AMNKEYKGRGVEFVGLSVEEPLTTREKVRDFAAQYRIDYRLGFSQRAWAASLMQGKTN
                     IPQLFVLRDGRVLNRFIGYNDTRPQQLRDAIEAALKAN"
     gene            766..1218
                     /locus_tag="VF240_00670"
     CDS             766..1218
                     /locus_tag="VF240_00670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7173782.1"
                     /translation="MELVAITESGEPAKPAPHTPPMTAEIVGATTRLYRAVGYEPPWI
                     GYLAIENGACVGTCGFKSPPRNNRVEIAYFTFPGHESRGVATRMASELILRALNKMPA
                     VTVAAQTLPEENASTSVLKKLRFRLVGSVEHPEDGLVWEWQLSAAPEG"
     gene            1336..2154
                     /locus_tag="VF240_00675"
     CDS             1336..2154
                     /locus_tag="VF240_00675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7173783.1"
                     /translation="MSAINPEMESLKTRLKAMWMTGDFGEVARHIATGAEEFIARLAL
                     EPGTRVLDVGCGSGNLSIPAARAGAIVTGVDIATNLLEQARARAASESLTIQFDEGDA
                     ESLPYADAAFDVVVSMFGAMFAPRPELVAAELVRVCRSGGRIAMANWTPDGFIGQMFK
                     LTGKYVPPPAAMPPPVKWGDEETVRERLRDGIADLQLTRRMCPFEYPFPPAEVVESFR
                     MYYGPTRRAFEALDSEGQAALRNDLERLWAEHNQATDGTTYGEGEYLEVIATRN"
     gene            2204..2884
                     /locus_tag="VF240_00680"
     CDS             2204..2884
                     /locus_tag="VF240_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173784.1"
                     /translation="MMMKTVLKASVFCWGIVVAFCFFFLIPFEAMGPVAAAAVSMSLG
                     GGALLWLGRYVYRLHKKEGFDVGEFMLAHHGFWKLFLGVVGLALFLIGGFWFLAPRET
                     ERRLEEGAMPVAAFLVLLFWFSLIFTFLGFSLVCFAESAAYLRIKAFKSGAGGFATSA
                     FFLALATLFCSLFLEVINDNFLRLSGATQNLILCLFALSVTVGGLLVGGFKDLEKLLP
                     EEELKRRV"
     gene            2896..3447
                     /locus_tag="VF240_00685"
     CDS             2896..3447
                     /locus_tag="VF240_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173785.1"
                     /translation="MKNHGVLSKWLRHDDQKDLFEVLVASAMNVVFLALSLLLLWPLG
                     RPLLAFRLAKGYGVLWGLTCVAALLGNRVQEFFRVNIYDRSNAYVISNLAVSCFLQVG
                     WSAFAALSVNHFVSGAPVWMTVTLYLVGVLSCLVAFFAVSSFYQGHIYKLVSLPLALL
                     SFIVFGVWPAGGRLMYGWFFELF"
     gene            3717..5771
                     /locus_tag="VF240_00690"
     CDS             3717..5771
                     /locus_tag="VF240_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX7173786.1"
                     /translation="MEIENEIKPERTVKFSLSKRPLIFFLVLSALIGCAVLRSSISTR
                     LDGLTLDEAYHIGAGVAYVRTGDFRLNPEHPPLVKLWTGAFVSDGYQLSPYRKLQDKN
                     DERNFVEEDVYLKNNPDVIQSRSRTAMFALNSLLLCLFALSARRMFGDIIALAATAFL
                     VIDPTTAAHLPVVMTDLPVALLSTTAVLLAVAAFRSWRAVELIFAAVALGLALSAKHS
                     GVITMAAIAIIGIVMAIFWTKETMPVVRLRRVGLVAAVLTGAMIVLWSFYLFRFSESS
                     ATSEEQFNRPLAEKISDVNSPRYKAGLNMMARGHLFPRAYIWGMADTIRAGAEGRARS
                     VFAFGNRYKKAPLYFFPGVIAVKLPLGLLLLTSIGAGLLIARNIPREFIAPLFGVIGL
                     AVLFLMVLATGSAYAGIRHALPVVPPLALLSSLAIYKAVESKSYLLRGVVTAALIGAL
                     LSAIPVLRPWEYYNETVGGTPNAYLYFSDEGIDISLRTKELAKYYNENLRTAGELPYI
                     LYPIRQAERKGRGLDWVGKNLERDAEKLDSESLSGTIIMGARQVRGKPAFREATPIAR
                     FGNLFVFRGTFYDPDTKVLRLTNRANNKIYSAEPDIEGAIKLLSEAVALDPKKYHLAV
                     ELGNQYLTVGKREEALRAYHIAKENAPPADDISELLTRQIERVKTEPLEQISPVRNPK
                     IE"
     gene            complement(5870..6202)
                     /locus_tag="VF240_00695"
     CDS             complement(5870..6202)
                     /locus_tag="VF240_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7173787.1"
                     /translation="MPRIEAVTATDERTLDAEVGRKLVLALEDGGVDILHRCGGNAKC
                     TTCRVEVIEGDAGEMGDAERNRLAMETGLAENVRLSCQVHVAGDMKVRVVNQASVKGI
                     DAGPRPAE"
     gene            complement(6351..6707)
                     /locus_tag="VF240_00700"
     CDS             complement(6351..6707)
                     /locus_tag="VF240_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX7173788.1"
                     /translation="MVATTVPTFALELTGAAVEEVKKFMSSEEGLPETAGLRVRVVPG
                     GCSGFQYSLNIEEASREGDHITETGGVRLFVDMFSAQYLNNVKIDYVTGMMGSGFTFT
                     NPNATGGCGCGTSFTA"
     gene            complement(6800..7891)
                     /locus_tag="VF240_00705"
     CDS             complement(6800..7891)
                     /locus_tag="VF240_00705"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX7173789.1"
                     /translation="MTDMAHITEADVYGALAQVKDPDLHKDIVRLGFVKDLKIEGGRV
                     SFRIVLTTPACPVREQLEGEARAVVAALPGVSSVDVTMDAEVPKGRGLGDKVVVEGVR
                     NIIAVSSGKGGVGKSTVAVNLAVSLARDGARVGLMDADVYGPNVPQMLGAGEMRPVID
                     VNKLVPIEAHGVRMMSMAVLKPGDEPMIVRGPILHGLVKQFLQDVKWGELDYLIVDMP
                     PGTGDVQLSLAQLVPVQGAVLVTTPQEVALSDVRRALRMFETVHVPVLGVVENMSYFV
                     APDTGKRYNIFGEGGGRRLAEQYGVPFLGEVPMGMDVREGGDRGVPVVVGAPDSPQAA
                     AFRRVAEEVARYVSIEAMKPELVILGKAR"
     gene            complement(7997..8650)
                     /locus_tag="VF240_00710"
     CDS             complement(7997..8650)
                     /locus_tag="VF240_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX7173790.1"
                     /translation="MVANLRHGLGASREEIVHQLRARGRVGADELAAALGVSKQCVRK
                     HLDVLEREGYVEHAPERGDRGRPAHVFRLTDKAGELFPRRYDLFAKAVLRQVGEVWGE
                     RGLNAVFCGCASETVGSLRPQLEGLNFDARVRRLTELLGEMGYEAEAERLEDGSYLLT
                     EWNCPQAEVAREYRQLCDQELIVYRDLLAAEVFRESRIAGGATHCAYRVLRPKRGDK"
     gene            9121..11469
                     /locus_tag="VF240_00715"
     CDS             9121..11469
                     /locus_tag="VF240_00715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7173791.1"
                     /translation="MQTKRHVTTHIPRAQKTRRRRKATTLLLLSLTLQPAQALSQGAA
                     RRTPPSRPVSADVRPAPADVSAGSVGVPFATLLALVRAEDERRWDESEFAPLLSDKRA
                     AVRRRAALAAGRIGNEGAVARLTTLLRADADEGVRATAAFALGEIESDAAADVLIASL
                     RTGKSVAERARAFEALGKIAAALPATQEERRKSLGEAILAALAAEQKGARSNNALVLA
                     GLTAVLRARPAGAGEAVAPFLSSADARVRADAANTLSRLRAKTAAAKLRELLTGDPDA
                     VVRSNAARALGAAEDVASLDALAARAAADTDQRVRISAVRALAQLKDARAVEPLVKRA
                     ETLFAPYRAAKTSAETRPPETNELLEIAAALGRLLPPAGNERALTWLRQFREAENFSA
                     PEVEVALAHVLSTQYLREPPLDALAGVLGGKAPRGRAASLKSWQSYSAVAQGLAEIAS
                     ASSAAGGNSAVSLKADAQMLLLALLGDPATPALAVPDVLRALAAFKSANHAETLRAKL
                     TDDDVIVRATAADLLGELDPDPTSARALAAALPRALSDKLNDAALSIVGALARQKTNE
                     SAEAIRTALAVPDYVVRRRAAALLKEMGRGGSAAESADTVATQFKEADYRRALGRTGR
                     SVRARVLTDKGAFTMELLPDEAPMNVDNFVELARRGYFNGVMFHRVVPNFVVQGGDPR
                     GDGNGGPGYQIRCEVNAVPYGRGAVGMALSGKDTGGSQWFVTHAPQPHLDGGYTVFGR
                     VVEGMDVVDRTARGDRITGVTVIEGKPPATGTERPTGRKAKP"
     gene            11534..13408
                     /locus_tag="VF240_00720"
     CDS             11534..13408
                     /locus_tag="VF240_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7173792.1"
                     /translation="MSCFFCATMLAIVCAARGATCLAQAAARPTPTPKPSSTPRPTPT
                     PAPRTLYRISLAIDFDRRTYEGTERVRWVNADDRPASVLFFHLYPNLRADEPQPQTRA
                     MMPTTTMTAPAAAAKPPAPNASADDASSPPASDEPGLEVMAVRAALPSGQPLVFTLDE
                     ARVTLRVQLREAVAAGGVAEIEIKFRGSVPEIDADETSLTAHLMQQVGAALQDTREQR
                     RARDINFHSRGVMLLGSFHPVLAARSGGDWRREVEPTLAAIAHADAADYEVEIVASAD
                     VALYTSGSDSIDAETSRQRSRRFTGEGLRGFAVVAGRALRSQERESAGVRVRAVYAPE
                     HERTARRVLDVSAEAVRVFAARFGPLPFKSFTVAEAPLVAGMGSAEFSGLSAIASAFY
                     ADFDSPAMRRLPEVVREQRASVEDSLEFAAAHMAAHQWWGAAVGSDAAREPVVDEALA
                     NWSALLYYQDARGAEPARVASDEQLRGVYEVYRTFGGEDMPANRPARDYRNSFQYAAV
                     VSAKGALMIEALRPLLGERRFFEALRGYYEKHRLGVATLDDLQEALALGVNTQQRRLV
                     ARLFERWLTERHGDEDIAPPNPQLASALGISLEPGAAKDRNAFSRFGRFFWRRMTRIR
                     "
     gene            complement(13482..>14282)
                     /locus_tag="VF240_00725"
     CDS             complement(13482..>14282)
                     /locus_tag="VF240_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7173793.1"
                     /translation="RDGTVNLEKTEFYVPNEYVFSLAAAHPQLFEPVISVNPYRADAV
                     EELEKWAGRGASMVKWLPNAMGIDPSDERCDPFYRKMRELGVVLLSHGGEEKAVEAEE
                     DQRLGNPLLLRRPLDHGVKCIVAHCAGLGYNEDLDDPTGKRTHNFDLFMRLMDDRRYD
                     GLVFGDISAMTQFNRIGASLSTVIEREDLQARLVNGSDYPLPAINLLIRTRPLARAGY
                     IDEDDRPLLREIYGFNPLLFDFVLKRRLHLPGTNKRLSAHVFMAHPDL"
BASE COUNT         2528 a         4628 c         4386 g         2740 t
ORIGIN      
        1 aactgccgga aaagcgcgca atcaagcagg ccaccagccg cggcaggcgc gtcaaactgc
       61 gaaacgtttc tggactgaga cgcgcatcgc cgtcgccgtc acggcgctgg ccgtgacggc
      121 tgtcctcggc ctcgcgacct gcggcccgtc gaagacgcag cgtgggccga accccgtcct
      181 cgacacagac cttcaactcg tggacggcgg cacgttccgg ctcgccgagt acgacggccg
      241 cgtgttggtg ctcaacttct gggcgacgtg gtgcgggccg tgccgctcgg agataccgca
      301 tctggtcgcg atgaacaagg agtacaaggg gcgcggcgtg gagttcgtcg gcctctcggt
      361 cgaggagccg ctgacgacgc gcgagaaggt gcgcgacttc gccgcccagt acaggattga
      421 ctaccgtctc ggcttctccc aacgagcgtg ggccgccagc ctgatgcagg gcaagacgaa
      481 catcccccag ctcttcgtcc tgcgcgacgg ccgcgtcctc aaccgcttca tcggctataa
      541 cgacacacgc ccgcagcagt tgcgcgacgc catcgaggcg gcgctcaagg caaactgacg
      601 acagacgaac ccgttggata cgtccccagc gttcacgtca tgcagccgac caaacatcac
      661 atgctttacg tcgaagacca cccgaccacg tggccaagtg gatcgagttg aagccgaggc
      721 cggaagcgtc ctctactact cccgtcatgc ctcgcaggaa cgcagttgga acttgtcgcc
      781 atcacagaga gcggagagcc ggcgaagccg gcgccccaca cgccgccgat gacagcggaa
      841 attgtggggg cgacgactcg tctgtatcgt gcggtcgggt atgaaccgcc ctggattggc
      901 tacctcgcaa ttgaaaacgg cgcttgcgtc ggcacgtgtg gcttcaagtc accgccgcgg
      961 aacaatcgcg tcgagatcgc ctacttcaca tttcccggac acgagtcccg cggcgtggcg
     1021 acacgaatgg cgtctgaact catcctccgg gccttgaaca aaatgcctgc cgtgacggtg
     1081 gcggcgcaaa ccctgcccga ggagaatgcc tcaacttccg tgttgaagaa gctgcggttt
     1141 cgcttagtag gcagcgttga acacccggag gacggactgg tgtgggagtg gcagctaagc
     1201 gccgctcccg agggataaca ccccattgct cccgaccact cgatagcatg gctttcatca
     1261 tccctactcg ccgagcaagt catgcgcggc tctgtctgtc gtcacgaaaa cccttgtttg
     1321 aaaggagctc cagcgatgag cgcaatcaat cccgagatgg aatctttgaa gacccggctc
     1381 aaggcgatgt ggatgaccgg cgatttcggt gaggtcgcca ggcatatcgc aacgggtgcg
     1441 gaagagttta tcgcccgtct ggcgctggag ccgggcacgc gcgtgctcga cgtgggttgc
     1501 ggcagcggca atctgtcgat accggcggcg cgggcgggcg caatcgtcac cggcgttgac
     1561 atcgccacga atctgctcga acaggcgcgc gcccgtgccg catccgaaag cctgacgatt
     1621 cagtttgacg aaggcgacgc ggagtcgctg ccgtatgcgg acgcggcctt tgacgtggtc
     1681 gtgagtatgt tcggagcgat gttcgcgcca cgccccgaac tggtggcggc tgaactggtg
     1741 cgcgtctgcc gaagcggcgg acgcattgcg atggcgaatt ggacgcccga cggattcatc
     1801 ggtcagatgt tcaagctcac cggcaaatat gtgccgccgc ccgccgcgat gccgccgccc
     1861 gtcaagtggg gcgacgagga aaccgtgcgc gaacgactgc gtgacggaat tgcggattta
     1921 caattgacgc ggcggatgtg tccgttcgaa tacccgtttc cgccggccga ggtggttgag
     1981 tcgttccgca tgtattacgg cccgacgcgg cgcgcgttcg aggcgctcga cagcgaaggg
     2041 caggctgcgc tgcgaaacga tctggagcgg ctgtgggcgg agcataatca agcaaccgac
     2101 ggcacgacgt atggtgaagg agagtacctc gaagttatcg ccacgagaaa ctaaaggtca
     2161 gtaacattta ccggatcggg taacgccgca agctcggaaa cggatgatga tgaagacggt
     2221 gctcaaagct agtgtgttct gctggggcat cgtcgtggcg ttctgtttct ttttcctcat
     2281 tccgtttgaa gcaatggggc cggtcgcggc ggcggccgtc tctatgtcgc tgggtggcgg
     2341 cgctctgctc tggttgggac gctacgtata tcgtcttcac aaaaaagaag gctttgatgt
     2401 cggcgagttt atgttggccc accacggctt ctggaagctg tttttgggcg tggtcgggct
     2461 ggcgttattt ttgatcggcg gcttctggtt tctcgccccg cgggagaccg aacggcgact
     2521 cgaagaaggg gcgatgccgg tcgccgcatt tctggtcctg ctgttttggt tttcgctgat
     2581 ctttacgttc ctcgggttct cccttgtgtg cttcgccgaa tcggccgctt atctgcggat
     2641 aaaagctttc aaatcggggg caggtggttt cgccacctcc gcatttttcc tggcgctggc
     2701 gacgcttttt tgctcgctgt ttctggaggt gattaacgat aattttctga ggctgtccgg
     2761 cgcgactcaa aacctgattc tctgtttgtt cgcgctctcc gtaaccgtcg gcgggcttct
     2821 cgtcgggggg tttaaggatt tggaaaaatt gttgcccgaa gaagagctga agaggcgcgt
     2881 ttaaaatcgg cgggtatgaa aaatcatgga gtgctcagca aatggctaag gcacgacgat
     2941 cagaaagact tgttcgaggt tcttgtcgcc agcgccatga acgtagtttt cctcgctctg
     3001 agcttattgc tgctctggcc tctgggcaga ccgttgctgg cgtttcgact ggcgaagggg
     3061 tacggcgtct tgtggggtct cacttgtgtg gcggccttgc tgggcaatcg agttcaagaa
     3121 ttctttcgag tgaatatcta tgatcgctcc aacgcttatg taatctcgaa tcttgccgtc
     3181 agttgctttc tgcaagtggg ctggtcggcc ttcgccgcgc tctccgtcaa tcacttcgtt
     3241 tcaggcgcgc ccgtctggat gacggtgact ctgtacctcg tcggggtgct ttcgtgtctc
     3301 gtcgctttct tcgcggtgtc ttcgttctat caagggcaca tctacaaact ggtcagcctg
     3361 cctctggcgt tgctcagctt catcgtgttc ggcgtatggc ctgccggcgg ccgcttgatg
     3421 tatggatggt tctttgaact tttttgagaa gccgaacggt ccgtgcgaga ggacacacct
     3481 gcgcctccaa tactgcaacg gcctaacaac ggcatgcact cgacgcctcg ccacggaatc
     3541 tttcatcaac gtcgaattgg ggcgcggttg atgtccggcg ttagcctgcc gacgcgaata
     3601 cttaaaagat cagttaaatc catcagaact gatattatct gaggcgaatt ttagaactct
     3661 ctcttctgaa tgcagcacca tctgtagtaa acgtccctcg caaaggaaca aactttatgg
     3721 aaattgaaaa tgaaataaaa cctgaaagaa ccgtaaagtt ttctttgtcg aagcggcctt
     3781 tgattttctt tctggtgttg tcggcgctta ttggttgcgc cgttcttcgc tcaagcatct
     3841 cgacgcggtt ggacggcttg acactggatg aggcttatca tatcggcgcg ggtgtggctt
     3901 atgttcggac cggcgatttt cggctcaatc ccgaacatcc gccgctcgtc aagttatgga
     3961 cgggggcttt tgtgtccgat ggttatcaac tttctcctta tcgcaaactt caggacaaga
     4021 acgacgagcg caattttgtc gaagaagatg tttatctcaa gaataaccct gatgtgattc
     4081 aaagccgttc gcggacggcg atgtttgctt tgaacagttt gctgttatgt ttgttcgcct
     4141 tgtcggcgcg gcgcatgttc ggagacatta ttgccttagc cgctaccgct tttttggtga
     4201 tcgacccgac gactgccgct catcttccgg tggtgatgac agatttgccc gtcgcacttc
     4261 tttcaacgac ggccgtcttg ctggcagttg cggcgtttcg ctcctggcgc gcggtcgagt
     4321 tgatttttgc cgcggtcgct ctcggattag ctttgagcgc gaaacactcg ggcgtcatca
     4381 caatggcggc aatcgcaatt atcggaatcg tgatggcgat tttttggacg aaggagacga
     4441 tgcctgtcgt tcgcctgcgt cgtgtcggat tagtggcagc ggttttaacg ggcgcgatga
     4501 ttgtcttgtg gagtttttat ctgttccgct ttagcgaaag ttcggcaaca tctgaagaac
     4561 aattcaaccg tcctttggcg gagaagatca gcgatgttaa ttcgccgcgt tacaaagccg
     4621 gattgaatat gatggcgcgc ggacatctgt tcccacgcgc gtatatctgg ggaatggcgg
     4681 acacgattcg cgccggtgcg gaaggacgcg cccgctccgt ttttgctttc ggcaaccgtt
     4741 acaagaaagc gcctctctat ttttttcccg gtgtgattgc cgtcaaactc ccgctcggtt
     4801 tactgcttct gacctcaatc ggcgcgggtt tactgattgc ccgcaatatt ccgcgggaat
     4861 ttatcgcgcc cctgtttggt gtgattggtt tagccgtgct ttttttgatg gtgctcgcca
     4921 caggttcggc ttacgcggga atcagacacg cgcttccggt cgtcccacca ttggctttac
     4981 tgtcatcact ggcaatttac aaggcggtcg aatccaaatc ttatcttttg cgcggcgtgg
     5041 ttactgcggc acttatcggt gctttgctgt cggcgattcc tgttctgcgt ccttgggagt
     5101 attacaacga aactgtcggc ggcacgccaa acgcctatct ttattttagc gatgagggca
     5161 ttgatataag tttgcggact aaggaattgg cgaaatatta taacgagaac ttgagaaccg
     5221 caggcgaact tccttacatc ctctatccta tcaggcaagc ggaaaggaaa ggtcggggac
     5281 ttgattgggt cggcaaaaat ctcgaacgcg acgcggaaaa acttgacagc gaatcgctca
     5341 gcggcacgat cattatgggg gcgaggcaag tccgcggcaa acccgctttc cgggaagcga
     5401 ctccgatagc gcgttttgga aacttgttcg tctttcgcgg cacgttttat gaccctgaca
     5461 caaaggtgtt aaggcttacc aatcgtgcca ataacaaaat ttattccgcc gagccggaca
     5521 tcgaaggggc aataaaactg ctgtccgaag ccgttgcact tgacccgaag aagtatcacc
     5581 tcgctgtgga actcggaaat cagtatctca ctgtcggcaa gcgcgaagaa gcactgcggg
     5641 cttaccacat tgccaaagaa aacgccccgc cagccgacga catcagcgaa ttgctcactc
     5701 gccaaattga gcgcgtgaaa accgaaccgc ttgaacagat ttcaccggtg cgtaatccaa
     5761 agattgaata ataaaaaaag tcagaagggc gacgggagca ctcccgtcgc ccttctgact
     5821 ttcatattta ccgtccgcgc cgttgcaggg acgggcagac tattcgcacc tactcggccg
     5881 ggcgcgggcc ggcgtcgatt cccttgacgc tcgcttgatt gacgacgcgc accttcatgt
     5941 cgcccgcgac atgcacctga caggagaggc ggacgttttc ggccagcccc gtctccatgg
     6001 cgaggcggtt gcgctcggcg tcacccatct cgcccgcgtc gccttcaatc acttcgacgc
     6061 ggcaggtcgt acatttggcg ttgccgccgc agcggtgcag gatgtcaacg ccaccgtctt
     6121 cgagcgcgag cacgagcttg cgccccactt cggcgtcgag cgtgcgctcg tcggtcgccg
     6181 tcacggcttc aatcctgggc atccctttag ttctcctctg aaagttgtcg ggccgtgaaa
     6241 gctcgccaag cccggcgggg tgagtttcgc gacgcgaacg gccccgaagc gcgccctgct
     6301 cggcgcgctt cggagtcgcc tcggtcttaa cggacgaacc gcccgcgtgc ttaggccgtg
     6361 aacgaggtgc cgcagccgca gccgcccgtg gcgttcgggt tggtgaaggt gaacccgctg
     6421 cccatcatgc ccgtcacgta atcgatcttg acgttgttca ggtactgcgc gctgaacatg
     6481 tccacgaaga gacgcacgcc gcccgtctcc gtgatgtggt cgccctcgcg cgaagcctcc
     6541 tcgatgttca ggctgtactg gaagcccgag cagccgcccg gcaccacgcg aacgcgcagt
     6601 cccgccgttt cggggagacc ctcctcgctc gacatgaact tcttcacctc ttccaccgcc
     6661 gcccccgtca gttcaagcgc gaatgtcgga acagttgttg ccaccatgat tctctctcct
     6721 cctaaataat tcggtcgttt gattgccgtc ctgtcagttt agatgcagga cttcggttaa
     6781 ttgaatgttt gccgccgcct tagcgcgcct tgccgaggat gaccagctca ggtttcatcg
     6841 cctcgatgga gacgtagcgc gccacctcct cggccacgcg ccggaacgcc gcggcctgcg
     6901 gcgaatcggg agcgccgacg acgacgggca ccccgcggtc gccgccctcg cgcacgtcca
     6961 tccccatcgg cacttcgccc aaaaacggca cgccgtactg ctcggccaga cggcgcccgc
     7021 cgccctcgcc gaagatgtta taacgcttcc ccgtgtccgg cgcgacgaag tagctcatgt
     7081 tctccacgac gccgagcacg ggcacatgca ccgtctcgaa catcctgagc gcccgacgca
     7141 cgtccgagag cgcgacctcc tgcggcgtcg tcaccagcac cgccccctgc acggggacga
     7201 gctgcgccag cgacagttgc acgtcgccgg tgccgggcgg catgtccacg atcaggtaat
     7261 caagctcgcc ccacttcacg tcctgcaaga actgcttcac cagcccgtgc aaaatcggcc
     7321 cgcgcacgat catcggctca tcgcccggct tgagcacggc catcgacatc atccgcacgc
     7381 cgtgggcctc gatggggact aatttgttca cgtcgatgac gggccgcatc tcgcccgcgc
     7441 cgagcatctg cggcacgttc ggcccgtaca cgtcggcgtc catcagtccg acgcgggcgc
     7501 cgtcccgcgc gagactcacg gcgaggttga cggccaccgt cgatttgccc acgccgccct
     7561 tccccgaaga gacggcgatg atatttctca cgccctcgac cacgaccttg tcgcccagac
     7621 cccgaccttt gggaacctcc gcgtccatcg tcacgtccac actgctgacg cccggcagcg
     7681 ccgcgacgac cgcgcgcgcc tcgccttcca actgctcacg caccgggcac gccggcgtcg
     7741 tcagcacgat gcggaagctg acgcggccgc cctcgatctt caaatccttg acgaagccaa
     7801 ggcgcacgat gtccttgtgc aggtccgggt ctttgacctg cgccagcgcg ccataaacat
     7861 cggcctcggt gatgtgtgcc atatcagtca tggtgataag tgatgagtga taagtgataa
     7921 gaaagaccgg aagctgttag ctcttagctg ttagctaatc gctaagagct aacagcttct
     7981 tatcacttat cactcatcac ttatcacccc tctttggtct cagaaccctg tacgcgcaat
     8041 gtgtcgcgcc gcccgcgatg cgcgactcgc ggaagacttc cgcggcgagc agatcgcgat
     8101 agacgatcaa ctcctgatcg cagagctgtc gatactcacg cgctacctcg gcctgcgggc
     8161 agttccactc cgtcagcaga taggagccgt cttcgagccg ctcggcctcg gcctcgtagc
     8221 ccatctcgcc gagcagttcg gtcagacgcc gcacgcgcgc gtcgaaattc agtccttcga
     8281 gttgcggcct gagcgacccc acggtctcgc tcgcgcagcc gcagaagacg gcgttcagcc
     8341 cgcgctcacc ccacacctcg cccacctgcc tgagcaccgc cttcgcgaac aaatcgtagc
     8401 gccgcggaaa aagctcaccg gccttgtccg tcagacggaa gacgtgcgcg ggccggccgc
     8461 ggtcgccgcg ctcgggcgcg tgctcgacgt agccctcgcg ctccagcacg tccagatgtt
     8521 ttctcacaca ctgcttggag acaccgagcg ccgccgccaa ctcgtccgcg cccacacgcc
     8581 cgcgggcgcg caactgatgc acgatctcct cccgcgaggc ccccagccca tgtctcaagt
     8641 tagcgaccat actcgaattt ccatgctact ttcacacaca tcttttgtca aactgccggt
     8701 tgtttaaatc attttacctg aggaaatctg gccttttcat aggtaagatg agcaaggatc
     8761 agaacaagca tgtgctttac aagatgctgt gctacatcgt ctgttgcgtt atacaatcga
     8821 ggtatgaaag agatatttag atgggtcatc aaggtgtccg tggtgctcct actagccttt
     8881 ctcctactgg taatattctt ccccctgctc gcaggcgaag agttcttccc gagataaatt
     8941 ttatgcatct gaacacgccg tacaatttct aatctttaac aacttaattc ggacggactt
     9001 gacacgcgcg ccccgtctcg ccgatacata cacacatccc cggacaagag cgcgaacggg
     9061 cggcgcgagg ccgcggccgt ccccgatgag tcgcgagaga ccttcggcat ccgaaactac
     9121 atgcagacca aacgacacgt aaccacgcac atccctcgcg cgcagaagac gcggcggcgg
     9181 cggaaggcca cgacgcttct ccttctgtcg ctcacgctcc agcccgcgca ggcgttgtcg
     9241 cagggagcgg cgcggcgaac gccgccgtcg cgtcccgtct cggccgacgt gcgcccggcc
     9301 ccggccgacg tgagcgccgg cagcgtcggc gtccccttcg caacccttct ggcgctcgtg
     9361 cgcgcggagg acgagcggcg ctgggacgag tcggagttcg cgcccctgct ctccgacaag
     9421 cgcgcggccg tccgccggcg cgcggcactt gccgccggcc gcatcggcaa cgagggcgcg
     9481 gttgcgcggt tgacgactct cctgcgcgcc gacgccgacg aaggcgtgcg cgcaaccgcc
     9541 gccttcgccc tcggcgagat cgagtcggac gccgcggcgg acgtgctcat cgcgtcgctg
     9601 cgcacgggca agtctgtcgc ggagcgtgcg cgcgcgtttg aggcgctggg taagatcgcc
     9661 gccgcgctgc ccgccacaca ggaggagcgg cgcaagtcgc tcggcgaggc catcctcgcc
     9721 gccctcgcgg ccgaacaaaa aggcgcgcgt tctaacaacg cgctcgtcct cgcgggactg
     9781 acggccgtgc tgcgcgcccg cccggccggc gcgggcgaag cggtcgcgcc cttcctctcc
     9841 tcggcggacg cgcgcgtgcg cgccgacgcg gctaacacgc tctcgcgtct gcgcgcgaag
     9901 acggccgccg cgaaactccg cgagctgttg acaggcgacc ccgacgccgt cgtgcgctcc
     9961 aacgccgcgc gcgccctcgg cgcggccgag gatgtcgcgt cgctcgacgc actggccgcg
    10021 cgcgccgccg ccgacacaga ccagcgcgtc cgcatcagcg ccgtccgcgc gctcgcgcaa
    10081 ctgaaggacg cgcgagccgt cgagccgctc gtcaaacgcg cggagacgct gttcgcgccc
    10141 taccgtgcgg cgaagacgag cgcggagacg cgcccgcccg agacgaatga gttgctggag
    10201 atcgccgccg cgctcggtcg cctactcccg ccggcgggca acgagcgcgc gctcacatgg
    10261 ctccggcagt ttcgggaggc cgagaacttt tcggcgcccg aggtcgaggt ggccctcgcg
    10321 cacgtcctct cgacgcaata cctgcgcgag ccgccgctcg acgcgctcgc cggcgtcctc
    10381 ggcggcaagg cgccgcgcgg ccgtgcggcg tcgctcaagt cgtggcagag ttattcggct
    10441 gtggcgcagg ggctggccga gatcgcttcg gcgtcgagcg cggcgggcgg caactccgcc
    10501 gtcagtctta aggccgacgc tcagatgctt ctgctcgcgc tcctcggcga cccggccacg
    10561 cccgcgctcg ccgtccccga tgtgctgcgc gccctcgccg ccttcaagtc cgccaaccac
    10621 gccgagacgc tgcgcgccaa actcacagac gacgacgtga tcgtgcgcgc cacggccgct
    10681 gacctcctcg gcgaactcga ccccgacccc acgagcgcac gcgcactcgc cgccgccctg
    10741 ccccgcgcgc tctccgacaa gttgaacgac gcggcgctct ccatcgtcgg cgcgctcgcc
    10801 cgtcagaaga cgaacgagtc ggccgaagcc atcaggacgg cgctggccgt gcccgactac
    10861 gtcgtgcgcc gtcgcgccgc cgcgctcctc aaagaaatgg gacgcggcgg aagcgcggca
    10921 gagagcgccg acacggtcgc cacgcagttc aaggaggctg actaccggcg cgcgctcggc
    10981 cgcacgggtc gcagcgtgcg cgcacgcgtc ctcaccgaca agggcgcgtt cacgatggag
    11041 ctgctgccgg acgaggcgcc gatgaacgtg gacaacttcg tcgaactcgc gcgccgcggc
    11101 tacttcaacg gggtcatgtt ccaccgcgtc gtcccgaact tcgtcgtgca gggcggcgac
    11161 ccgcgcggcg acggcaacgg cggccccggc tatcagattc gctgcgaggt caacgccgtc
    11221 ccctacggcc gcggcgccgt cggcatggcg ctctcgggca aagacacggg cggctcgcag
    11281 tggttcgtca cgcacgcgcc gcagccgcac ctcgacggcg gctacaccgt cttcggccgc
    11341 gtcgtcgaag gcatggacgt ggttgaccgc accgcgcgcg gcgaccgcat cacgggcgtc
    11401 acggtgattg aagggaagcc gccggcgacc ggcactgagc ggccgaccgg gcggaaggcc
    11461 aagccataag accaagactt ggggggattc cagcgacggg gcggtaaacg tgttgagtcc
    11521 caaacgaata tccgtgtcct gcttcttctg cgcgacgatg ctcgccatcg tctgcgccgc
    11581 gcgaggagcg acgtgtctcg cgcaggctgc cgcgcggccg acgccgacgc cgaaaccatc
    11641 atcgacgccg cgaccgacgc cgacgcccgc cccgcgcacg ctctaccgca tcagcctcgc
    11701 catcgacttc gaccgccgaa cctacgaggg caccgagcgc gtgcgctggg tcaatgccga
    11761 cgaccgcccg gcctccgtcc tcttcttcca cctctacccg aacctgcgcg ccgacgaacc
    11821 gcagccgcag acgcgcgcga tgatgccgac gaccacgatg accgccccgg ccgccgccgc
    11881 gaagccgccc gcgccgaacg cttcggcgga tgacgcgtct tcgccgcccg catccgacga
    11941 gccgggtctc gaagttatgg ccgtgcgcgc cgccctgccc tcggggcagc cgctcgtctt
    12001 cacgctcgac gaagcgcgtg tcacgttgcg cgtgcagttg cgcgaagcgg tggcggccgg
    12061 cggcgtggcg gagattgaaa tcaagtttcg gggcagtgtc ccagagatcg acgcggatga
    12121 gacgagcctc accgcgcacc tcatgcagca ggtcggggcc gcgcttcagg acacgcgcga
    12181 gcagaggcgc gcgcgcgaca tcaacttcca ctcgcgcggc gtaatgcttc tgggcagctt
    12241 ccaccccgtc ctcgccgcgc ggtcgggcgg cgactggcgg cgcgaagtcg agccgacact
    12301 cgccgccatc gcccacgccg acgcggccga ctacgaggtc gaaatcgtcg cgtccgccga
    12361 cgtggcgctc tacacttccg gcagcgactc catcgacgcg gagacgagcc gtcagcgcag
    12421 ccgacgcttc acaggcgagg ggctgcgcgg cttcgccgtg gtggcggggc gcgccctgcg
    12481 ttcacaggag cgcgagtcgg cgggcgtgcg cgtgcgcgcc gtctacgcgc cggagcacga
    12541 gcgcacggcc cgtcgcgtgc tcgacgtgtc ggcggaggcg gtgcgggtgt ttgcggcgcg
    12601 cttcgggccg ctccccttca agtcgtttac ggtcgccgaa gcgccgctcg tcgccgggat
    12661 gggcagcgcc gagttctcgg gactgtcggc catcgccagc gccttctacg ccgacttcga
    12721 ctcgccggcc atgcgccgcc tgcccgaggt cgtgcgcgag cagcgggcga gcgtggagga
    12781 cagtctggag ttcgccgcgg cgcacatggc cgcgcaccag tggtgggggg cggccgtggg
    12841 cagcgacgcc gcgcgcgagc cggtggttga cgaggcgctc gccaactggt cggcgcttct
    12901 ctactatcag gacgcccgcg gcgcggagcc ggcgcgcgtt gcctcggacg aacagttgcg
    12961 cggcgtctac gaggtctacc gcacgttcgg cggagaggac atgccggcca accgccccgc
    13021 gcgcgattac cgcaactcgt tccagtatgc ggccgtcgtc tccgcgaagg gcgcgctcat
    13081 gatcgaagcg ctccggccgc tcttgggcga gcggcgcttc ttcgaggcgc tgcgcggcta
    13141 ctacgagaag caccggctcg gcgtcgccac gctcgacgat cttcaggagg cgctcgcgct
    13201 cggcgtcaac acgcagcagc gacgactggt cgcgcgtctc ttcgagcgct ggctcaccga
    13261 gcgccacggc gacgaagaca tcgcgccgcc caacccgcag ttggcctccg ccctcggcat
    13321 ctccctcgaa cccggcgcgg ccaaagaccg caacgccttc tcgcgcttcg gccgcttctt
    13381 ctggcggcgc atgacgcgca tccgctaaat caatttgccg caattgccgg gggacagtac
    13441 cgctcgcggt actgtttcgg caccctcaac cgcaacccgc tttagagatc ggggtgcgcc
    13501 atgaagacat gcgcggacag gcgcttgttc gtaccgggga ggtggaggcg gcgtttgagg
    13561 acgaagtcga agagcagcgg gttgaagccg taaatctcgc gcaggagggg gcggtcgtcc
    13621 tcgtcgatgt agccggcgcg cgcgagcggc ctcgtcctga tgagcaggtt gatggcgggc
    13681 agcgggtagt cgctgccgtt gacgaggcgc gcttgcaggt cttctctttc gatgacggtc
    13741 gagagcgacg cgccgatgcg gttgaactgc gtcatcgcgg agatgtcgcc gaagacgagg
    13801 ccgtcgtagc gacggtcgtc catcagacgc atgaagagat cgaagttgtg cgtgcgcttc
    13861 cccgtcgggt cgtccaggtc ctcgttgtag ccgagtcccg cgcagtgcgc gacgatgcac
    13921 ttgacgccgt gatcgagcgg ccggcgcaac agcagcgggt tgccgaggcg ctgatcctct
    13981 tccgcctcga cggccttctc ttcgccgccg tggctcagga gcaccacccc caactccctc
    14041 atcttccggt aaaacggatc acaccgctcg tcggacgggt cgatgcccat cgcgttcggc
    14101 agccacttga ccattgaagc gccgcggccg gcccacttct caagctcctc gaccgcgtcc
    14161 gcgcgatagg ggttgacgga gatgaccggc tcgaacagtt gcgggtgcgc ggcggcgagt
    14221 gagaagacgt attcgttggg gacgtagaac tccgtcttct ccagattcac cgtcccgtcg
    14281 cg
//
LOCUS       DASVES010000018        10437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_951068, whole genome shotgun sequence.
ACCESSION   DASVES010000018 DASVES010000000
VERSION     DASVES010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951068"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1970)
                     /locus_tag="VF240_00730"
     CDS             complement(60..1970)
                     /locus_tag="VF240_00730"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7173794.1"
                     /translation="MQRRFKFVNVLVTSLLVAQLGVPCFAFQTGSGALTQQQQRFSDG
                     QLPPPRFADPERARKLAAAFPEIERIFTSYVERRHMPGAVMGIIIDGELAWVKTAGVR
                     DTQGRAPVTPDTVFRIASMSKSFTAMAILKLRDEGKLSLDDPVSNYVSALAGLPYPTK
                     DSPALTIRHLLTHSEGFPEDNPWGDRQLAQTNETMDAWMRAGIPFSTAPGTAYEYSNY
                     GFAILGRVVESAARKPYADYVREQILLPLGMRASAFEEREVAREHVTKGYRWEDNAWK
                     EEPILAHGSFGAMGGLWTSARDLARYVSFLMAAFPARDESERGPVRRSSAREMQQAWR
                     AQPASAFRGTVDAPLQLGVAAYGYGLGASQDCRFAHVVGHGGGLPGYGSLMRWLPEHG
                     VGLIAMSNLTYGGFGAVFNEAIAALHKTGALLPRVAQASPALLSAQKDVTQLITKWDD
                     ALASRITADNLLMDETAERRAARLKELSAKHGACRPATSIDAENALRGRWRMTCERGW
                     LDVFITLAPTSQPRVQLIGVQSTFPPSEEVTKAVESVMRLMSAWDQKTAESLAGTGFD
                     VERMRKQIGAASQWGACKVGETLAGDGKRDTLVRLNCDRGKLGLDIALDPAGKRLTGF
                     NLLTSRVDRCVP"
     gene            complement(2163..3071)
                     /locus_tag="VF240_00735"
     CDS             complement(2163..3071)
                     /locus_tag="VF240_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX7173795.1"
                     /translation="MKVIVTGSNGLVGRALTGQLLAEGHEVTTLVRSGSRTAGEEGVR
                     PARWDPSADSIDAAALEGHDAAVHLAGESIAEGRWDDEKKSRIRESRVKGTRLLAETL
                     ARLEHKPSVLVSASATGFYGNRGEETLTEESASGDDFLSEVCREWEKATLPASQAGVR
                     VVHLRFGIILSREGGALQKMLTPFKLGAGGRIGSGRQYWSWIDLTDALGVIRHAITDE
                     SLRGAVNAVAPGAVTNDEFTKTLGGVLGRPTLLSVPAFAARLAFGEMADALLLGGQRV
                     EPRRLTESGYKFAHPELESSLRHVLR"
     gene            complement(3099..4598)
                     /locus_tag="VF240_00740"
     CDS             complement(3099..4598)
                     /locus_tag="VF240_00740"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7173796.1"
                     /translation="MTAERKTVTAADVARIIVAKLEGNESARVSDVTHDSRKAGPGFL
                     FVAVAGESFDAHRFIPQVLEAGAAGVISERERPEGFEGAAWMRVADVRRAQALAAAEV
                     QGHPSRELNLVGITGTNGKTTTTYLVAAVGEAAGEPVALTSTVEYRVGGERIKAERTT
                     PEASDTQRLLRRAVEAGCRLAVMESSSQAIDLHRCDELHYAAAVYTNLTRDHLDYHIT
                     MDAYFDAKRRLFDGRLGSRPRASVINIDDERGARLAQELEAEGARVVRYALKTEAEVT
                     ARDVEHSLEGTSLRLVTPEGERLLRSPLVGRPHVYNILATAGTGLALGYSFETVARAV
                     ETCGGAPGRFERVPHGGDFAVVVDYAHSDDALVNVLRTAREVTRGRVVTVFGCGGDRD
                     RSKRAPMGEAAARLSDVVILTSDNPRTEDPERIFADIEEGLRHTSKPYLKIADRREAI
                     MRAVAEARGGDLVMIAGKGHEDYQIIGKEVSHFDDREVAREALAARGAR"
     gene            complement(4678..5490)
                     /locus_tag="VF240_00745"
     CDS             complement(4678..5490)
                     /locus_tag="VF240_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7173797.1"
                     /translation="MARASLLLVSVILIILPVFQPLALSSPATPGAATRLRVMSYNVH
                     VGIGADKRNDLARIAEVIRRERPHLVGLQEIDRGVERTKRVDQMVELARLTGMEYAFA
                     HNLDYQGGQYGVAVLSRLPILAVDHRRLRHMREAERRGLLRVEVSVAGKTVNFVTTHL
                     DYQNHDNRLFETRQLTDALKGINTPLIVVGDFNDEPSGDSYKFMLEQFADAWVSASAP
                     GDGLTYPADKPAKRIDYVFYRSGPLRARRAWIPQTQASDHLPLVVDLELSPR"
     gene            complement(5706..6857)
                     /locus_tag="VF240_00750"
     CDS             complement(5706..6857)
                     /locus_tag="VF240_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEX7173798.1"
                     /translation="MSTEAGEMEGKATAATRLVSLDAFRGITVAGMILVNNPGTWGAI
                     YSPLAHADWHGWTPTDLIFPFFLFIVGVAIALALSRRAESSGTKRDLYLKILRRSAVI
                     FALGIILAGFPFFELSTIRIPGVLQRIAVCYLFASVIFLNTNWRTLAIITAALLVVYC
                     LMMTLIPVPGYGPGDLASKEWNLAAYIDRAVFGPHIWSQGKVYDPEGLLSTIPALATT
                     LTGVLTGLWLRSRETHAGKVAGMFVAGAVCIVLGWAWGFWFPVNKALWTSSYVLLTTG
                     MALQFLAFCYWLIDIKGYRRWALPFIIFGVNALALYFLSGIAADLLGTIKMSAADGQQ
                     ISLQQLIYQNLFASWAAPKFASLLFAICFVLVWLGLMAILYRKKIFIKV"
     gene            complement(7041..8897)
                     /locus_tag="VF240_00755"
     CDS             complement(7041..8897)
                     /locus_tag="VF240_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7173799.1"
                     /translation="MAARHNIITRSIFTYRSGRKVVGVVARAVLCLTCVVGGSSPAAV
                     RSQKGAGRDAGARTREAESRWVEETLKGLSVRERIAQTVFAAVQADYMNVGGARFAEV
                     RKLVEESRVGGLVVRGGSPNEVAALANELQRLSKAPLLFAADYERGLRMQMKSGTPFT
                     NNMGVAAAGEARAARAQARVTAEEARAVGVHWLFGPVADINNNPENPVINTRSFGEDP
                     RRVAEFVTAAVAGTREGGALSTAKHFPGHGDTAADTHIGLATIRADRARLERIELVPF
                     RAAVEAGVDAVMTAHVAVPDLTGDDLPATLSPKMTTELLRRELKFDGLIVTDSLGMGA
                     ITKGFPGGEAAVRALKAGADVLLNPPDVKAAIDAVEEAVRRGEVAEARVDESVRRILR
                     AKFRVGLSEKKTVELAAVNSIVERPESVREAQETAERSLTLLRNEGALLPLDPARGAR
                     TLFIVVAADDDPEEGRTLVPQITRRVSGAKVHRADPRTTDAEYDAYVAEAAKAEVVVV
                     APFVKRAASKGTVALPGRQAEFVRRLIELKKPLAVVGFGSPYLIRQFPEARVYLTTYA
                     IEDIAQAAAARALFGETTITGRLPVSIPGLFALGAGLRVEARKQDGRTNGQD"
     gene            9060..10103
                     /locus_tag="VF240_00760"
     CDS             9060..10103
                     /locus_tag="VF240_00760"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7173800.1"
                     /translation="MHTKAVSIMLEEIAQQPEALARTISEEKEKVRRLGAFLRGRDID
                     LIVLVARGSSDNAALFGRYLLEITTGIPVALAAPSVHTLYGARLRLSRALVVGVSQSG
                     EGEDINRVLEMSRADGAYTVGITNEPASAMTKLVDETLLMHGGRERSVAATKTYTGQL
                     LHFYMLAEALGGAALGYEKIPEHAEHALALRSAVEELVERYVFMENCVVVGRGLLYAN
                     SYEMALKLMETCYVVAERFSSADFLHGPLAIVERHFPIFVFAPPGVTQQSVRELLERL
                     RELRADTLTFTSDDDIAGLSTRAVRMPFDVPEVLAPIPYIIPAQLFAALLANAKGLDP
                     DAPRSLAKVTRTL"
     gene            10235..>10437
                     /locus_tag="VF240_00765"
     CDS             10235..>10437
                     /locus_tag="VF240_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7173801.1"
                     /translation="MKLLILGGTLFLGRHIVESALRDGHEVTLFNRGQHNPELFPEVE
                     KLRGDRDGGLDALRGRSWDAVIDT"
BASE COUNT         1740 a         3402 c         3479 g         1816 t
ORIGIN      
        1 agcagacccg ctcgctaccg ctcgcggtac tgttagcaaa tcgctcgctt cgtcggtaat
       61 caaggaacgc aacggtcaac gcgggatgtg agcaggttga agccggtgag tcgtttgccc
      121 gcggggtcga gagcgatgtc caggccgagc ttaccgcggt cgcagttgag cctgacgagg
      181 gtgtcgcgtt tgccgtcgcc ggcgagcgtc tcacccacct tgcaggcgcc ccactgcgag
      241 gccgcgccga tttgtttccg catccgctcc acgtcgaacc ccgtcccggc caacgactcg
      301 gccgtcttct gatcccacgc gctcatcagt cgcatcaccg actcgaccgc cttcgtcacc
      361 tcctcgctcg gcgggaaggt tgactggacg ccgattagct gaacgcgtgg ctgactggtc
      421 ggcgccagcg taatgaaaac gtcgagccag ccgcgctcgc aagtcatccg ccagcgccca
      481 cgcagcgcgt tctcggcgtc gattgacgtg gccgggcggc acgccccgtg cttcgcgctc
      541 aactctttca ggcgcgcggc gcggcgctcg gcggtttcgt ccatcaagag gttgtcggcc
      601 gtgatgcgcg aggcgagggc gtcgtcccac ttcgtgatga gttgcgtgac atccttttgc
      661 gcggacagca gcgcgggcga ggcttgcgcg acgcgcggca gcagcgcgcc cgtcttgtgg
      721 agcgcggcaa tcgcttcgtt gaagacagcg ccgaagccgc cgtaggtcag gttgctcatg
      781 gcgatgaggc cgacgccgtg ctcgggcagc cagcgcatga gcgagccgta gccgggcagt
      841 ccgccgccgt ggccgacgac gtgtgcgaag cggcagtcct gagaagcgcc gaggccgtag
      901 ccgtaggccg cgacgcccaa ctggagcggc gcgtccaccg tgccgcggaa ggcgctcgcc
      961 ggctgagcgc gccacgcctg ctgcatctca cgcgcgctgc tgcgccgcac cggcccgcgc
     1021 tccgactcgt cgcgcgcggg gaaggccgcc atgaggaacg agacgtagcg ggcgaggtcg
     1081 cgcgcggaag tccacaggcc acccatcgcg ccgaacgagc cgtgcgcgag aatcggctcc
     1141 tccttccagg cgttgtcctc ccaacgatat cccttcgtga cgtgctcgcg cgccacttcc
     1201 ctttcctcga aggcgctggc gcgcatgccc aacggcagca gaatctgctc gcggacgtaa
     1261 tcggcataag gcttgcgcgc ggcgctctcg acgacgcggc cgaggatggc gaagccgtag
     1321 ttcgagtact cgtaagcggt gccgggcgcg gtcgagaaag ggatgccggc gcgcatccac
     1381 gcgtccatcg tctcgttcgt ctgcgcgagc tggcggtcgc cccaggggtt gtcttcgggg
     1441 aagccctcgg agtgtgtgag caggtgtcgg atggtgagcg cgggcgagtc ctttgtcggg
     1501 tacgggaggc cggcgagcgc gctcacgtaa ttagacacgg ggtcgtcgag cgagagcttg
     1561 ccctcgtcgc gcagcttgag gatggccatc gccgtgaagc tcttcgacat cgacgcgatg
     1621 cggaagaccg tgtcgggcgt gacgggcgcg cgcccctgcg tgtcgcgcac cccggcggtc
     1681 ttcacccacg ccaactcgcc gtcgatgatg atgcccatga cggcgccggg catgtgccgc
     1741 cgctcaacat agctcgtaaa gatgcgctcg atctccggga acgccgcggc cagcttgcgt
     1801 gcgcgctcgg ggtcggcgaa gcgcggcggc gggagttgtc cgtcggagaa ccgctgctgc
     1861 tgttgtgtga gtgcgccgct tccagtctgg aatgcgaagc acgggacgcc gagctgggcg
     1921 actaatagtg aagtgactaa gacgttaacg aacttgaatc ggcgctgcat ctgaagcctc
     1981 cggtaatcga ggaaataatt aacatggatg gacaggatgg ataggatgaa agatttcaca
     2041 tctcaagtct gaaatctcaa atctgaaatc tcaaatctga aatttgaaat tagtttttcc
     2101 ttctcctgcc catcctgctc atccatgtta attcatcacg tgccaatctc acctgtcgtg
     2161 atttagcgca ggacgtgccg cagcgagctt tccaactcag gatgggcgaa cttgtagccc
     2221 gactccgtca gccgccgcgg ctcgacgcgc tggcctccga gcaggagcgc gtcggccatc
     2281 tcgccgaagg cgagacgcgc ggcgaaggcc gggacgctta agagcgtggg acgcccgagc
     2341 acgcccccga gcgttttggt gaactcgtcg ttcgtgacag cgccgggcgc gacggcgttg
     2401 accgccccgc gcaggctctc gtccgtaatc gcgtggcgga tgacgccgag cgcgtccgtg
     2461 aggtcgatcc agctccaata ctgtcggccg ctgccgatgc gcccgccggc gccgagctta
     2521 aagggcgtga gcatcttctg tagcgcgccg ccttcccggc tgaggatgat gccgaagcgc
     2581 aggtgaacga cgcgcacgcc ggcctggctc gccggaagcg tcgccttctc ccactcgcgg
     2641 cagacttcac tcaggaagtc gtcgcccgac gcgctctcct ccgtcagcgt ctcctcgccg
     2701 cggttgccgt agaatccggt ggccgaggcg ctgaccagaa ccgagggctt gtgctccagc
     2761 ctcgccagcg tctcggccag cagacgcgtc cccttcacgc ggctctcgcg gatgcggctc
     2821 ttcttctcgt cgtcccagcg cccctcggca atcgactcgc cggcgaggtg gacggccgcg
     2881 tcgtggcctt cgagcgccgc cgcgtcgatg ctgtccgccg acgggtccca gcgcgccggc
     2941 ctgacgcctt cctcacccgc cgtccgtgag ccgctgcgca cgagcgtcgt tacctcgtga
     3001 ccttccgcga ggagctgccc cgtgagcgcg cgcccgacga gtccgttcga gcctgtgacg
     3061 ataactttca tccgacctcc ttgaaaacaa ctttcacttc agcgcgcgcc cctcgccgcc
     3121 agcgcctcgc gcgccacttc cctgtcgtcg aagtgggaga cttccttgcc gatgatctgg
     3181 tagtcctcgt gtcccttgcc ggcgatcatc acgaggtcgc cgccgcgcgc ctcggcaacg
     3241 gcgcgcatga tcgcctcgcg ccggtcggcg attttgagat acggcttcga cgtatggcgc
     3301 aggccctctt cgatgtcggc gaagatgcgc tcggggtctt cggtgcgcgg gttgtcggag
     3361 gtgaggatga ccacatcact caagcgagcg gcggcctcgc ccatcggcgc gcgcttcgag
     3421 cggtcgcggt cgccgccaca gccgaagacg gtgacgacac ggccgcgcgt tacctcccgc
     3481 gcggtgcgta ggacgttgac gagcgcgtcg tctgagtgcg cgtaatcgac gacgacggcg
     3541 aagtcgccgc cgtgcggcac ccgctcgaag cgccccggcg ctccgccgca ggtctcgacg
     3601 gcgcgcgcga ccgtctcaaa cgagtagccg agcgcgaggc ccgtgccggc ggtggcgaga
     3661 atgttgtaga cgtgcgggcg tccgacgagc ggcgagcgca gaagccgctc gccctcgggc
     3721 gtgacgagac gcaggctggt gccttcgagc gagtgctcca catcgcgcgc ggtcacttcc
     3781 gcctcggtct tgagcgcata gcggacgacg cgcgcgccct ccgcttcaag ctcttgggcg
     3841 aggcgtgcgc cgcgctcgtc gtcaatgttg atgacggagg cgcgtgggcg cgagccgagg
     3901 cggccgtcga agaggcggcg cttggcgtcg aaataggcgt ccatcgtgat gtgatagtcg
     3961 aggtggtcgc gggtgaggtt ggtgtagacg gccgccgcgt agtgcaactc gtcgcagcgg
     4021 tggaggtcga tggcctgcga ggacgactcc atgacggcca gccggcaacc cgcctccacg
     4081 gcgcggcgca acagacgctg cgtgtcgctc gcctccggcg tcgtgcgctc ggccttgatt
     4141 ctctcgccgc cgacgcggta ctcgaccgtg ctcgtcagcg ccacaggctc gcccgcggcc
     4201 tcgccgacgg ccgcgaccaa gtacgtggtc gtcgtcttgc cgttcgtgcc ggtgatgccg
     4261 acgaggttca gctcgcgcga cgggtggccc tgcacctcgg ccgcggcgag cgcctgcgcg
     4321 cgcctcacgt ccgcgacgcg catccacgcc gcgccctcga atccttcggg ccgctcgcgt
     4381 tcagaaatca cgccggccgc gcccgcctcc aacacctgcg ggatgaaacg gtgcgcgtcg
     4441 aagctttctc ccgccacggc gacgaagagg aagcccggcc ccgccttgcg cgagtcgtgc
     4501 gtcacgtccg acacgcgcgc cgactcgttg ccttcgagct tcgcgacgat gatgcgcgcc
     4561 acgtcggcgg cggtcacggt ttttcgttct gccgtcaacg aaaattttct ccagcaaaca
     4621 aaaggttgaa attgagagcg gggcgattat agaacagaca cacgcgcggc ggacaggcta
     4681 ccggggagac agctccagat cgacgacgag cggcaggtga tccgacgcct gcgtttgggg
     4741 aatccacgcg cggcgggcgc gcaaggggcc gcttcggtag aagacgtagt cgatgcgctt
     4801 ggcgggcttg tcggcggggt aggtcaggcc gtcgccgggg gcggacgccg acacccaggc
     4861 gtcggcgaac tgttcgagca tgaacttgta ggagtcgccg gaaggctcgt cgttaaaatc
     4921 cccgacgacg atgaggggag tgttgatgcc cttcagcgca tcggttagct ggcgggtctc
     4981 gaagaggcgg ttgtcgtggt tctggtagtc gagatgtgtg gtgacgaagt tgacggtctt
     5041 cccggcgacg cttacttcga cgcggagaag gccgcggcgc tcggcctcgc gcatgtgtcg
     5101 gaggcgacga tggtctacgg cgaggatggg caggcgcgag aggacggcga cgccgtactg
     5161 gccgccctga tagtcgaggt tgtgcgcgaa ggcgtactcc atgccggtga ggcgcgcgag
     5221 ttcaaccatc tgatcgacac gctttgtgcg ctccacgccg cggtcgattt cctgtaagcc
     5281 gacgaggtgg ggccgctcgc ggcggatgac ttcggcgatg cgcgcgaggt cgttacgctt
     5341 gtcggcgccg atgccgacgt ggacgttgta ggacatcacg cgcaggcgcg tcgcggcgcc
     5401 gggcgttgcg ggcgacgaca aggcgagcgg ctggaagacg ggcaggatga tgaggatgac
     5461 actgactaag agtaatgatg cgcgagccac tgtgaagctg agttgagtgc gtcgcgggtt
     5521 gcggttcacc gcggaggcgc agtagacgca gagggggcgc agaggaaggc aaaaggcaaa
     5581 agttaaaagg gaaaagtaag aaagcctctt cacttttgcc ttttgccttt ttacttttgc
     5641 cttcacaagt cctctgcgcc tctgcggact ctgcgttaat tctttgaggc gcatcgctgt
     5701 tcacctcaaa ccttgatgaa gattttcttc cggtaaagaa tcgccatcaa cccgagccag
     5761 acgagcacga agcagatggc gaagaggagc gaggcgaact tgggcgcggc ccaggaggcg
     5821 aagaggttct ggtaaatgag ctgttggaga ctgatctgtt ggccatcggc ggcggacatc
     5881 ttgatcgtcc ccagaaggtc ggcggctatc ccgctgagga agtaaagggc gagcgcgttg
     5941 acgccgaaga tgatgaaggg gagcgcccag cgccggtagc ccttgatgtc gatgagccag
     6001 tagcagaagg cgagaaactg gagcgccatg cccgtcgtca gcaggacgta ggaactcgtc
     6061 cacagcgcct tgttgacggg gaaccagaag ccccacgccc accccaacac gatgcagacc
     6121 gcgcccgcga cgaacatccc ggcgacttta cccgcgtgtg tttcgcgcga gcggagccac
     6181 agccccgtga gcacgccggt gagcgtcgtg gcgagcgccg ggatggtgct cagcagcccc
     6241 tccgggtcgt aaactttccc ctgactccag atgtgcgggc cgaagaccgc gcggtcgatg
     6301 taggccgcga ggttccactc cttgctcgcg agatcgcccg gcccgtagcc gggcacgggg
     6361 atgagggtca tcatcagaca atagacgacg agcagcgcgg ccgtgatgat cgcgagtgtg
     6421 cgccagttcg tattcaggaa gatgactgac gcgaagagat agcagacggc gatgcgctgg
     6481 aggacaccgg ggatgcgaat ggtcgagagt tcaaaaaagg ggaagccggc gaggatgatg
     6541 ccgagcgcga agatgacggc ggagcggcgg aggattttca gatagaggtc gcgcttcgtg
     6601 cctgagcttt cggcgcggcg gctgagcgcg agggcgatgg cgacgccgac gatgaagagg
     6661 aagaacggga agataaggtc ggtcggcgtc cagccgtgcc agtcggcgtg ggcgagcggc
     6721 gagtagatgg cgccccacgt gccggggttg ttaacgagga tcatgccggc caccgtgatg
     6781 ccgcgaaacg cgtcgagtga caccagacgc gtcgctgccg tggccttccc ttccatctcg
     6841 ccggcttcag tgctcatgtt tcctccctcg cctctccagc attagagtcc attgcgagac
     6901 ctctcagcgc gagtcacgtt cgcggctcgc gctgggaggt ttagacagaa ttgacaggac
     6961 gtacaggaca aagaaagaat tgatgtcaga tttgagattt gagatttgag atttcaaatc
     7021 ttctcatcct gtccgtcctg ttaatcctgt ccattcgttc gcccgtcctg cttcctcgcc
     7081 tcgacacgca gccccgcgcc gagcgcgaag aggccgggga tgctcacggg cagacggccc
     7141 gtaatcgtcg tctcgccaaa gagggcgcgc gcggccgccg cctgtgcgat gtcttcaatc
     7201 gcgtaggtcg tcagatagac tcgcgcctcc gggaattgac gaatcaggta cgggctgccg
     7261 aagccgacga ccgccagcgg cttcttcaac tcgatcaggc ggcgcacgaa ctcggcctgc
     7321 ctaccgggca gcgccaccgt ccccttgctc gccgcacgct tgacgaaggg cgcgacgacg
     7381 accacctccg ccttcgcggc ctcggcgacg taggcgtcgt actcggcatc ggtggtgcga
     7441 gggtcggcgc ggtgtacctt cgcaccactc acgcgtctcg tgatctgcgg gacgagcgtg
     7501 cgtccctctt cggggtcgtc gtccgccgcg acgacgatga agagcgtgcg cgcgccgcgc
     7561 gccgggtcga gcggcagcag agcgccctcg ttgcgaagca acgtcagaga ccgctcggcc
     7621 gtctcctgcg cctcgcgcac gctctcgggt cgctcgacga tggagttgac agccgcgagt
     7681 tcaaccgtct tcttctccga cagccccacg cggaacttcg cgcgcaggat gcgtcggacg
     7741 gattcatcca cgcgcgcctc cgcgacttcg ccgcgccgga cggcctcctc gacggcgtcg
     7801 attgccgcct tcacgtccgg cggattcagg agcacgtcgg cgcccgcctt cagcgctcgc
     7861 acggccgcct cgccgccggg gaagcctttc gtgatggcgc ccatcccgag cgaatcggtg
     7921 acgatgaggc cgtcgaactt tagctcgcgg cgcagcaact cggtcgtcat cttcggcgag
     7981 agcgtggcgg gcaaatcgtc gcccgtcagg tcggggacgg cgacgtgcgc cgtcatcacc
     8041 gcatcgacgc cggcctcgac ggcggcgcgg aacgggacga gttcgatgcg ttcgagacgg
     8101 gcgcggtcgg cccggatggt ggcgagtccg atgtgcgtgt cggcggccgt gtcgccgtgg
     8161 ccggggaaat gtttcgcggt ggagagcgcg ccgccttcgc gcgtgccggc cacggccgcc
     8221 gtaacgaact cggccacgcg ccgcgggtct tcgccgaagc tgcgcgtgtt gatgaccggg
     8281 ttctcggggt tgttgttgat gtcggcgacg gggccgaaga gccagtggac gccgacggcg
     8341 cgcgcctcct ccgccgtgac ccgcgcctgc gcccgcgccg cccgtgcttc gcccgccgcc
     8401 gccacaccca tgttgttcgt aaacggcgtg cccgacttca tctgcatccg caggccgcgc
     8461 tcgtagtcgg cggcgaacaa cagcggcgcc ttcgacagac gctgcaactc gttcgcgagc
     8521 gctgccactt cgttggggct gccgccgcgc acgaccagcc cgcccacacg gctctcctcg
     8581 acgagctttc taacctcggc gaagcgagcg ccgccgacgt tcatgtagtc agcctgcacg
     8641 gccgcgaaga ccgtctgcgc gatgcgctcg cgcacagaca gccccttcaa agtctcttcg
     8701 acccagcgcg actccgcctc tcttgttctc gcgccggcgt cgcgcccagc gcccttctgc
     8761 gagcgaaccg ccgcgggcga cgagcctccc acgacgcagg ttagacaaag gacggcgcgg
     8821 gcgacaacgc ccacgacctt gcggccgctt cgatatgtga agatgcttcg agtgatgatg
     8881 ttgtgtcggg ctgccatcgt tgtggcgtgt ggccgtgccg ttgttctgtc tcgccgcgag
     8941 gcgggcggag gacgacgcgg tagtgatata gttcagccga gcggcgacaa ttaaacacga
     9001 cccggtttcg gtgtcaacgt cgccgcctca atgatcgccc caacccgccc cgcaaggaga
     9061 tgcacacaaa agccgtgtcg atcatgctcg aagagattgc ccagcagccg gaggccctgg
     9121 cgcggacgat ctcggaagaa aaggaaaagg tgcggcgcct cggcgccttt ctccgtggcc
     9181 gcgacatcga tctcatcgtc ttagtcgcgc gcggctcctc cgacaacgcc gccctcttcg
     9241 gccgctacct gctggagatc accaccggca tccccgtcgc cctcgccgcc ccgtccgtcc
     9301 acacgctcta cggcgcgcgc ctgcgtctgt cacgcgcgct cgtcgtcggc gtctcacagt
     9361 ccggtgaggg cgaggacatc aaccgcgtgc tggagatgtc gcgcgccgac ggagcctaca
     9421 ccgtcggtat caccaacgag ccggcctcgg cgatgacgaa gctcgtagac gagacgctgc
     9481 tgatgcacgg cgggcgcgaa cgctccgtgg cggcgacgaa gacctacacg gggcagctcc
     9541 tgcacttcta tatgctcgcc gaagcgctcg gcggcgcggc cctcggctac gagaagattc
     9601 ccgagcacgc ggagcacgcc ctcgccctgc ggagcgcggt cgaggagctg gtcgagcgtt
     9661 acgtcttcat ggagaactgc gtcgtggtgg ggcgcggtct cctgtacgcg aattcctacg
     9721 agatggccct gaagctgatg gagacctgct acgtcgtggc cgagcggttc tcgtcggccg
     9781 actttctcca cggccccctc gccatcgtcg agcgccactt cccgatcttc gtcttcgccc
     9841 cgccgggcgt cacgcagcag agcgtcagag agcttctgga acgcctgcgg gagctgcgcg
     9901 ccgacacgct caccttcaca tccgatgacg acatcgccgg cctctcgaca cgcgccgtcc
     9961 gcatgccctt cgacgtgccc gaggtgctcg cgcccatccc ctatatcatc ccggcgcaac
    10021 tcttcgccgc gcttctcgcc aacgccaagg gtctcgaccc cgacgccccg cgctcgctcg
    10081 cgaaggtgac gcggacactt tgagatttat taacatggat ggacaggatg agcaggatga
    10141 atacaaagag aaaaaatgat ttcagatttg agatttcaaa tttcttatcc tccccatcct
    10201 gcttatccat gttaattttt ctgaggctcg aaagatgaaa cttctgattc ttggcgggac
    10261 gctgttcctt ggccgtcaca tcgtcgagtc ggcgctgcgt gacgggcacg aggtgacgct
    10321 cttcaatcgg ggccagcaca acccggagct tttcccggag gttgagaaac tgcgcggcga
    10381 ccgcgacggc ggtctcgacg ccttgcgcgg acggagttgg gacgccgtga tcgacac
//
LOCUS       DASVES010000019        10257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_173539, whole genome shotgun sequence.
ACCESSION   DASVES010000019 DASVES010000000
VERSION     DASVES010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173539"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1326
                     /locus_tag="VF240_00770"
     CDS             169..1326
                     /locus_tag="VF240_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173802.1"
                     /translation="MLKTLFLAAALTFTVVAAFVAPVGEPLFARSAAAQSAAVVIPLE
                     GLDPVLLIQGKEAQGDEKFSVTRGRFQYLFAGAETKAAFEREPARYEIQLGGACARMG
                     PSTQGNPDLYAVHDGRIYLFGSPACVKAFKEAPDKYLETAGAWASGGAPAPTEESRRK
                     GRALVEKAVEAVGGAARLDALVSYQEKATTSAHSPQGVTEIKTSMTRVYPGKYRDERT
                     LSFGTIATVVTPAAGFFATARGGGDMIDEQRAAYEKQFARNVLTLLRARRDAGFAASA
                     AGPGKAGETAIEQVDVAAGGASARLGIDPATGRVLTLSYRGRGPGGMFGDIVQTFSDF
                     RAVEGLTLPFKTAGTFNGEPSPAHTSAVESIVINAPVPPSLFEKPRPAGGQ"
     gene            1366..2643
                     /locus_tag="VF240_00775"
     CDS             1366..2643
                     /locus_tag="VF240_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7173803.1"
                     /translation="MKSETLKRKTIRTATRRLSVLCLLFACAASASAQANDWPQWGGP
                     QRNFVSGAKGLAAAWPEGGPRRLWSRELGEGYSAIAAEGERLYTMYRRGAQDVVVALD
                     AATGKTVWEYAYDAPFSADYSMENGPGPHATPTIAGERVFTAGPTGKLHALDKKTGKL
                     LWSHDLMSEYKGTIRVNGYACSPLVYRDTVIMQVGGAGNSLMAFRQKDGGVAWKRHDF
                     KNSPSSPLVINVDGQDQLVAFMYDEVVGVEPQTGELLWSHPHKTEFGLNVSTPVWGEG
                     NLLFISSSYGGGSRGLRLTRGREGKTAVEEVWAHALMRVHYGNAVRLGDFVYASSGDF
                     GTAPFTALNVRTGKIVWRDRTLARASLLAADGRLILLDEDGHLILATPTPEGLKVHSK
                     AELLKSNAWTAPTLAGTRLYVRDRKNVLALDLK"
     gene            2843..3976
                     /locus_tag="VF240_00780"
     CDS             2843..3976
                     /locus_tag="VF240_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7173804.1"
                     /translation="MAESFQSDAAPARRLWLKRAAVVFGIWAFFAVLTATQVYIGLRS
                     EGMNHSFGRLLAWQLSNWSIWALFTPVILRLGQRFPVTRATWPQRVLTHLCFCVLLAA
                     VHAALHNYLTVLIRPWDEMTNERPLLDMFWGKLTSQLHISVLIYGTILGVGYAFNYYV
                     KFREREARASQLESQLAQAQLHALKMQLHPHFLFNTLNGISGLVRDGSNKAAVQMIAG
                     LSDLLRHTLDTEGQQEVPLRRELEFLELYLDIQQMRFPDRLRVQMEIAPETLDAEVPN
                     LILQPLVENAIRHGIAPRAAPGTVGVRARRDERLLEIKVYDDGPGLRPNAPTGEGGGI
                     GLANTRARLAQLYGAGHRFDVRDRPEGGVEATIVVPFRLSREQ"
     gene            4247..5029
                     /locus_tag="VF240_00785"
     CDS             4247..5029
                     /locus_tag="VF240_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7173805.1"
                     /translation="MDDEPLARQNIRLLLEADAEVEIVGEAGDGAAAVKAIRKLSPDL
                     VFLDIQMPEMSGFDVLAALDAEHLPAVVFVTAFDRYALRAFEVHALDYLLKPFDDARF
                     EKALRQAKSQIRGREIGRLSERLLALLEERDARGPAAASAGRAPARNYLTRLLVKSAG
                     RVFFLKADDIDWVEAADYYVKLHVGRKGHLLRESMSELEARLDPDKFLRIHRSTIVNL
                     DRVREMHPLSGGEYTVVLHDGTQLKLSRSRREQLQKLLNLGD"
     gene            complement(5089..5757)
                     /locus_tag="VF240_00790"
     CDS             complement(5089..5757)
                     /locus_tag="VF240_00790"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7173806.1"
                     /translation="MAERTRVLLVRHGQSMGNAERRFGGHSATPLSALGRAQAEATAR
                     ALQTERVTAIYSSDLQRAVETAEPLARLTGLEIKRTEALRERSVGHMEGLTFEEAAQL
                     HPDEYAALLRRDFEHVLTGGESYRQMLDRSSRELDRAIQEHAGGALALFSHTGTICIL
                     ALHLMGALDAPTLRPVWISSSNCGITRFSIHHGGLVRVLAVNDTRHLADVDSTASGSE
                     RMRL"
     gene            complement(5842..6918)
                     /gene="prfA"
                     /locus_tag="VF240_00795"
     CDS             complement(5842..6918)
                     /gene="prfA"
                     /locus_tag="VF240_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7173807.1"
                     /translation="MLEKLEQIEARYEALTQELALPEVYTDQAAYSKVAKQHRALGEI
                     VGKYREWKSLGEELVGARELLESADDDEMREMARAEAAELEERIERAGEEMRVLLLPR
                     DPNDEKNVILEIRAGTGGDEATLFAAEMLRMYGRYAERQGWRMQILEASESGIGGVKE
                     AIAMIEGERVYSKLKHESGVHRVQRVPQTETSGRIHTSAITIAVLPEAEEVDVKIEAK
                     DLRIDTFCSSGPGGQSVNTTYSAVRITHLPSNLVVSMQDEKSQIKNREKAMRVLRARL
                     QELEEQKQHDALASERRSMVGSGDRSEKIRTYNFKENRVSDHRIGLTVHQLDLVMEGQ
                     LDEFINALVSHYQAEKLKAETVAA"
     gene            complement(7123..8337)
                     /locus_tag="VF240_00800"
     CDS             complement(7123..8337)
                     /locus_tag="VF240_00800"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX7173808.1"
                     /translation="MSTNERDLIVGGQAVIEGVMMRTPGAYAIAVRRADGTITHTGAP
                     LPKWGEKYPYFKLPVLRGAAVLVQSMALGIKALNYSASEAFADAEVEEAKEVKVALTP
                     AVVEGEGDFAGITGGVPGLFPVPTKKRPEDEMKGGGGAVFGSIAFALLFNVLIFIVAP
                     LLLTNALYVALGWDTPRTQQAAGVNQTAQTSSATSAAPETGSMATTTAQGEPWYKRAY
                     GSVRGYLKPVEVTPLFNLIDGVIRMSFFLIMIFSFSLMKDIRRVFEYHGAEHKTVFNW
                     ESGLPLTVENARVQPRQHPRCGTSFLMVVMLVSIVLFSVVKFDSLLYNMLVRIALLPL
                     VAGLSYEIIRVSAKREGSFVFKLITRPGVWLQNITTQEPDDEQLEVAIYALKESLKFE
                     PQPAEAVAAPLS"
     gene            complement(8682..8882)
                     /gene="rpmE"
                     /locus_tag="VF240_00805"
                     /pseudo
     CDS             complement(8682..8882)
                     /gene="rpmE"
                     /locus_tag="VF240_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            9169..>10257
                     /locus_tag="VF240_00810"
     CDS             9169..>10257
                     /locus_tag="VF240_00810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7173809.1"
                     /translation="MTDEPNKPPDDAAGDTPERTDARPGAPRTDDKSPSPGDEVERAG
                     TLPPPPDAGGPRPTPESQAALTGKAPSGSSVAGTTVPAASVGADETVGSVSAGKSEAT
                     TSAETPEQKKARLIAEHKAKLAAKAAAAAEGAGTEVNKDVPGGASPAVGDPTQSPSQS
                     DLEKAAKIAEAKARAAAAKEAGAAAAGAAAGAPKAPVKKKDEGPKPVDASDNPLVRRL
                     RERLGDGLLGAETFLNQLSIRVERAHNVETCRALRDDAETPFDYLSDVTCVHWPENFD
                     APFEIVYNLYSINGNERVRVKVNADAEGGVESMTGVWPAANWMEREVFDLYGVQFQNH
                     PDLRRLLLPPDWEGHPLRKDFPLEFMENN"
BASE COUNT         1802 a         3393 c         3308 g         1754 t
ORIGIN      
        1 cgtaagccat tcaacccggc gggccgccct cgtccgctat tcactgcacg aatcgcgcca
       61 ctcaccgcac ccacccgtcg tttcactgaa tatccaaaac accgcgcgtg cgccgggcgt
      121 aaactctgct ctacagtcga tggttcggtc ttaaggaggc ccaaagaaat gttgaagaca
      181 ctgtttctgg cggccgcgtt gacgtttact gtcgtcgccg ccttcgtcgc gcccgtcggc
      241 gagcctttgt tcgcgcggag tgcggcggcg cagagtgccg ctgtcgtcat cccgctcgaa
      301 ggtctcgacc cggtcttgtt gattcagggc aaggaggcgc agggcgacga aaagttctcg
      361 gtcacgcgcg ggcggttcca atacctgttc gcgggcgcgg agacgaaggc cgccttcgag
      421 cgcgagccgg cgcgctacga gattcaactc ggcggcgcct gcgcgcgcat ggggccatcg
      481 acgcaaggca accccgacct ctacgccgtc cacgacgggc gcatctacct cttcggcagc
      541 cccgcctgcg tgaaagcgtt caaggaagcg cctgacaaat atctggagac ggcgggcgcg
      601 tgggcctcgg gcggtgcgcc ggcgccgacc gaggagtcgc ggcgcaaagg gcgcgcgctc
      661 gtcgagaagg cggttgaggc cgtgggcggc gcggcgcgtc tcgacgcact cgtgagctat
      721 caggagaagg cgacgacctc ggcgcattcg ccgcagggcg tgaccgagat caagacttca
      781 atgacgaggg tctaccccgg caagtatcgc gatgagcgca cgctttcgtt cgggacgata
      841 gccacggtcg tcactcctgc cgccggcttc ttcgcgacgg cgcgaggcgg cggcgacatg
      901 atcgacgagc agcgcgcggc ctatgagaag cagttcgccc gaaacgtcct cacgctcctg
      961 cgcgcgcggc gcgacgcggg cttcgcggcc tcggccgccg ggccgggcaa ggcgggcgag
     1021 acggcaatcg agcaggtaga cgttgcggcc ggcggcgcga gcgcgaggct cggcatcgac
     1081 ccggcgacgg ggcgcgtgct cacgctctcg tatcgcggtc gcgggccggg cggcatgttc
     1141 ggcgacatcg tgcagacgtt ctccgacttc cgcgccgtcg aaggtttgac gctgcccttc
     1201 aagaccgccg gcacgttcaa cggcgagcct tcccccgcgc acacgtccgc ggtcgagtca
     1261 atcgtcatca acgcgcccgt tccgccgtcg ctcttcgaga agccacggcc ggcgggcggg
     1321 cagtagttgt tgttgttgta gcagtagcag cggggaagta cggcgatgaa atcggaaact
     1381 ctgaaacgaa aaacgattcg cacggcgacg cggcgactga gcgtcctatg cctgttgttc
     1441 gcgtgcgctg cctccgcctc ggcgcaggcc aacgactggc cgcagtgggg aggcccgcag
     1501 cgcaacttcg tctccggcgc gaagggtctc gccgccgcgt ggcccgaggg cggcccgcgc
     1561 cgtctgtggt cgcgcgaact cggcgagggc tattcggcca tcgcggccga gggcgagcgc
     1621 ttgtacacga tgtaccggcg cggcgcgcag gacgtggtcg tcgccctcga cgccgcgacc
     1681 ggcaagaccg tctgggagta cgcctacgac gcgcccttct cagccgacta ctcgatggag
     1741 aacgggccgg ggccgcacgc gacgccgacc atcgccggcg agcgcgtctt caccgccggc
     1801 cccacgggca aactccacgc gctcgacaag aagacgggaa aacttctgtg gagccacgac
     1861 ctgatgtccg agtacaaggg caccatccgc gtcaacggct acgcttgtag tccgctcgtc
     1921 taccgcgaca cggtcatcat gcaggtcggc ggcgcgggca actccctcat ggccttccgg
     1981 cagaaggacg gcggcgtcgc gtggaagcgc cacgacttca agaactcgcc ctcgtcgccg
     2041 ctcgtcatca acgtggatgg gcaggatcaa ctcgtcgcct tcatgtacga cgaggtcgtc
     2101 ggcgtcgagc ctcagacggg tgagcttttg tggagccacc cgcacaagac cgagttcggc
     2161 ttgaacgtga gcacgcccgt ctggggcgag gggaacctgc tgttcatctc ctcgtcttac
     2221 ggaggcggga gccgtgggct gcgactgacg cgcggcaggg aagggaagac cgcggtcgaa
     2281 gaggtgtggg cgcacgcgct gatgcgcgtc cactatggca acgccgtccg cctcggcgac
     2341 ttcgtctacg cgtcgagcgg cgacttcggc acggcgccct tcacggcgct caacgtcagg
     2401 acgggcaaga tcgtctggcg cgaccgcacc ctcgcgcgcg cctcgctcct cgcggccgac
     2461 ggccgcctca tcctcctcga tgaggacggc catctcatcc tcgccacgcc aacgcccgag
     2521 gggctgaagg ttcactcgaa ggcggaactg ctcaagagca acgcgtggac ggcgccgaca
     2581 ctcgccggca cacgtctcta cgtgcgcgac cgcaagaacg tcctcgcgct cgacttgaaa
     2641 tagagatttt tattttgagt gaatggcaag tcggttcacc gcggaggcgc agaggacgcg
     2701 gagactacgc agagggctga gcgtttcact caacgttgcg tcagagcgtc ggccctctgc
     2761 gcctcctctg cgtcctttgc gcctccgcgg tgagttaatt ctccgaacac tcaagctgaa
     2821 agcgatataa actgacccag tgatggcgga atcctttcag agcgacgccg cgccggcgcg
     2881 gcgtttgtgg ttgaagcgcg cggcggtcgt cttcggcatc tgggcattct tcgccgtgct
     2941 cacggccaca caggtctaca tcggcctgcg ctccgagggc atgaaccact ccttcggccg
     3001 gctgctcgcg tggcagttgt ctaactggag catctgggcg ctcttcacgc cggtcatcct
     3061 ccggcttggg caacgtttcc ccgtcacgcg cgcgacgtgg ccgcaacgcg tgctcacgca
     3121 cctctgcttc tgcgtcctgc tcgcggccgt acacgccgcc ctccacaact acctgacggt
     3181 tctcatccgt ccgtgggacg agatgacgaa cgagcggccc ctcctcgaca tgttctgggg
     3241 caagctcacg agccagctcc acatcagcgt gctcatctac ggcaccatcc tcggcgtcgg
     3301 ctacgccttc aactactatg tcaaattccg cgagcgcgag gcccgcgcct cacagctcga
     3361 atcacagctc gcgcaggcgc agctccacgc gttgaagatg cagctccatc cacacttcct
     3421 cttcaacaca ctgaacggca tcagtggcct cgtgcgcgac ggcagcaaca aggcggccgt
     3481 gcagatgatc gccggcctga gcgacctcct gcgccacacg ctcgacaccg aaggccagca
     3541 ggaggtgccg ctccgccgcg agctggagtt cctcgaactc tacctcgaca tccagcagat
     3601 gcgcttcccc gaccgcctcc gcgtccagat ggagatcgcg cccgagacgc tcgacgccga
     3661 agtgcccaac ctcatccttc aaccgctcgt cgagaacgcc atccgccacg gcatcgcccc
     3721 ccgcgccgcg cccggcaccg tgggggtgcg cgcgcgacgc gacgagcggc tgctcgaaat
     3781 caaggtctac gacgacggcc ccggcctgcg ccccaacgcg ccgacgggcg agggcggcgg
     3841 catcggcctc gccaacaccc gcgcccgcct cgcacagctc tacggcgccg gccaccgctt
     3901 cgacgtgcgc gaccgcccgg agggcggcgt cgaggccacc atcgtcgtcc cgttcagatt
     3961 gtcgagggag cagtagccgc gatttaataa tcgtaggggt agggcaagtc cgcggcccgt
     4021 gctgcgaaaa gaatttacat ggatgggcag gatgatcagg ataagagatt tgaaatctca
     4081 aatctgaaat ctcaaatcag ctctttcttc atcctgcacg tcttgtccat ccatgtagat
     4141 tcctttgatg ggcaacgggc cgcggacttg ccctgcccct acagttgtaa tctagctgtc
     4201 acacgtgaat atggaagaag gcgggaaaaa gatcagaacg ttgatcgtgg acgacgagcc
     4261 gctggcgcgt cagaacatcc gtctgctgct cgaagccgac gccgaggtgg aaattgtcgg
     4321 cgaggccggc gacggcgcgg cggcggtcaa agccatccgc aagctctcgc ccgacctcgt
     4381 cttcctcgat atccagatgc ccgagatgag cggcttcgac gtgctcgccg cactcgacgc
     4441 cgagcatctg ccggccgtcg tcttcgtcac cgccttcgac cgctacgcgc tccgcgcctt
     4501 cgaggtacac gcgctcgact acctgctcaa gcccttcgac gacgcgcgct tcgagaaggc
     4561 gctccgtcag gccaaatcgc aaatcagggg gcgcgagata ggccgcctca gtgagcgcct
     4621 gctcgcgctg ctcgaagaac gcgacgcgcg cggcccggcc gccgccagcg cggggcgcgc
     4681 gcccgcccgc aactacctga cgcggctgct ggtgaaatcc gcgggccgcg tcttcttcct
     4741 caaggccgac gacatagact gggtcgaagc cgccgactat tacgtcaagc tgcacgtcgg
     4801 gcgcaagggc cacctcctgc gcgagtcgat gtcggagctt gaggcccgcc tcgaccccga
     4861 caagttcctc cgcatccacc gctccaccat cgtcaacctc gaccgcgtcc gcgagatgca
     4921 tcccctctcc ggcggcgaat acacggtcgt cctccacgac ggcacccagc tcaaactcag
     4981 ccgcagccgc cgcgagcagt tgcagaagct actcaacctc ggcgactaga aaacagtacc
     5041 gcgagcggaa gcctcgcggg tctggccgcg cgcgtggcgg gcctctcatc acagccgcat
     5101 ccgctcgcta ccgctcgcgg tactgtccac gtcagcgagg tgccgcgtgt cgttaacggc
     5161 gaggacgcgg acgaggccgc cgtgatgaat agagaagcgc gtgatgccgc agttggagga
     5221 ggaaatccag acggggcgca aggtcggtgc gtcgagcgcg cccatgaggt ggagggcgag
     5281 gatgcagatg gtgccggtgt gggagaagag ggcgagggcg ccgccggcgt gttcctgaat
     5341 cgcgcggtcg agttcgcgtg aggagcggtc gagcatctgc cggtagctct cgccgcccgt
     5401 gaggacgtgt tcgaagtcgc ggcgcaggag cgcggcatac tcatcggggt gtagttgggc
     5461 ggcctcctcg aaggtcagcc cctccatgtg cccgacgctg cgctcgcgca gcgcctccgt
     5521 tcgtttgatc tccagccccg tcagacgtgc cagcggctcg gccgtctcga cggcgcgctg
     5581 gaggtcgctc gaatagatag ctgtcacacg ctccgtctgg agggcgcgcg ccgtcgcctc
     5641 ggcttgtgcg cggccgagcg cggagagcgg cgtcgccgag tgtccgccga agcgacgctc
     5701 ggcgttaccc atcgactgcc cgtgacgaac aagtaagact cgcgttctct ctgccatgtg
     5761 cacggattgg gcaaagggga aagggtaagg gggaagggaa caataattcc tttacccctt
     5821 accccttacc ctttcccctt cttacgccgc gaccgtttcg gccttgagtt tttccgcctg
     5881 atagtgggag acgagggcgt tgatgaattc gtcgagctgg ccttccatga cgaggtcgag
     5941 ctggtggacg gtgaggccga tgcggtggtc ggagacgcgg ttctccttga agttgtaggt
     6001 gcgtatcttc tcggagcggt cgccggagcc gaccatcgag cggcgctcgg aggcgagcgc
     6061 gtcgtgctgc ttctgctctt cgagttcctg aagccgcgcg cgcagcacgc gcatggcctt
     6121 ctcccgattc ttgatctgcg acttctcgtc ctgcatcgag acgacgaggt tcgacggcaa
     6181 gtgtgtgata cgcacggccg agtaggtcgt gttcacactc tggccgcccg gccccgagga
     6241 gcagaaggtg tcgatgcgga ggtctttcgc ctcgatcttc acgtccacct cttcggcctc
     6301 gggcagcacg gcgatggtga tggccgaggt gtggatgcgg ccgctcgtct ccgtctgcgg
     6361 gacgcgctgg acacggtgga cgcccgactc gtgcttgagc ttcgagtaga cccgctcgcc
     6421 ctcgatcatg gcgatggctt ccttgacgcc gccgatgcct gactcggagg cttcgagaat
     6481 ctgcatccgc cagccctgac gctcggcgta gcggccgtac atcctgagca tctcggcggc
     6541 gaagagcgtg gcctcgtctc cgcccgtgcc ggcgcggatt tcaaggatga cgttcttctc
     6601 gtcgttcggg tcgcgcggca gcagcaggac acgcatctcc tcgcccgcgc gctcgatgcg
     6661 ctcttcgagc tccgcggcct cggcccgtgc catctcgcgc atctcatcgt cgtcggccga
     6721 ctccagaagc tcgcgcgccc cgacaagctc ctcgccgagc gacttccact cgcgatactt
     6781 gccgacgatc tcgcccagcg cacggtgctg cttggccacc ttggaatagg ccgcctgatc
     6841 cgtgtacacc tccggcaacg caagctcctg cgtcagagct tcgtagcgcg cctctatctg
     6901 ttccagtttc tctaacatat ccgaatcctg tagggagagt tcaagtccgc ggcccgtccg
     6961 gcagaaacaa tttacatgga tgggcaggac gggcaggatg aagaaagaga tgatttcaga
     7021 tttcagattt caaatcttct catcctgtta accctgtcca tccatgtaaa tttcttccgt
     7081 atcgcgaagg gcagggacaa gccctgcccc tacaaaatac ctctatgaga gaggcgcggc
     7141 gacggcctca gccggctgcg gctcgaactt gagcgactcc ttgagcgcat agatggccac
     7201 ttcgagctgt tcgtcgtccg gctcctgcgt ggtgatgttc tggagccaga cgccgggccg
     7261 cgtaatcagc ttgaagacga agctgccctc gcgcttcgcc gacacgcgga tgatctcgta
     7321 ggagagaccg gcgacgagtg gcagcagcgc gatgcggacg agcatgttgt agaggagcga
     7381 gtcgaacttg acgacggaga acagcacgat ggagacgagc atcacgacca tcaggaaact
     7441 cgtcccgcag cgggggtgct ggcgcggctg cacgcgcgcg ttctcgaccg tcagcggcag
     7501 ccccgactcc cagttgaaca ccgtcttgtg ctccgcgccg tgatactcga agacgcgccg
     7561 gatgtccttc atcagcgaga agctgaagat catgatgagg aagaaggaca tgcggatgac
     7621 gccgtcgatg aggttgaaca ggggcgtcac ctcgacgggc ttcagatagc cgcgcacgct
     7681 tccataagcg cgcttgtacc acggctcgcc ctgagccgtc gtcgtcgcca tgctgccagt
     7741 ctcaggcgcg gcggacgtgg cgctgctcgt ctgcgcggtc tgattgacgc cggcggcctg
     7801 ctgcgtgcgc ggagtgtccc agccgagggc gacgtagagg gcgttggtca gcagcagcgg
     7861 cgcgacgatg aagatgagga cgttgaagag gagcgcaaag gcgatggagc cgaagacggc
     7921 gccgcctccg cccttcatct cgtcctcggg ccgcttcttc gtgggcacgg ggaagagtcc
     7981 gggcacgccg cccgtaatcc ccgcgaagtc gccctcgccc tcgaccaccg cgggcgtgag
     8041 cgcgaccttg acctctttag cttcctccac ctcggcgtcg gcgaaagcct cgctcgccga
     8101 gtagttcaaa gccttgatgc cgagcgccat cgactgcacg agcaccgccg cgccgcgcag
     8161 aacggggagc ttgaagtacg gatacttctc accccacttc ggcagtggcg cgcccgtgtg
     8221 cgtgatcgtg ccgtcggcgc ggcgcacggc gatggcataa gcgcccggcg tccgcatcat
     8281 cacgccctcg atgacggcct gcccgccgac gattaaatct ctctcgttgg tgctcacgtg
     8341 cgacctcgaa aatcgtgaat cgtcaatcgt caatcgtgct cgtgtgtctc agcttgagag
     8401 gtaaatcgca gcgcggccgt gcgccgacta cgacttacga ttcacgtttg acgatttacg
     8461 gctttaaaag aagacgccgg cgtttcgggg gcggtcgccc gtgcgcgttc gtgcgcgcac
     8521 gggtgtgttg accgccgaag gaaacgtcgg cgccctgatt ttggactgct aatactgacg
     8581 gcgaatgatt cgcgtcatcg aaatgccctg tcgaaacatt actgcgcggc gacgccctcg
     8641 gcgggcgcgc tggctgcttc gggcgcgggg gccgtctcgg tcttcttgcc gtagcgtttg
     8701 ttgaagcggt caacgcggcc ggccgtgtcg atgagcttct gcttgccggt gaagaacggg
     8761 tggcaggccg aacagatttc gacgccgagg tcgcccttgc gcgtcgaacg ggtcgtccac
     8821 ttctcgccgc aggcgcagtg gacgttgatc tcgtggtaat cgggatgaat tccttgcttc
     8881 atttttttat tcctcgccag aaggtgaaca atcgaggccg cttccgaatt tcaaatgata
     8941 cttgccggcc tcccgtatcg tcaagttttg gatgcactta accggcattc ggtggccgcg
     9001 cgcgctgtcg tacaagttta ttgacaacgc cgcgggcgcg tgttacaaaa acccgctgtc
     9061 acgccttata gaagcatctt tggtaaagcg cacaagtcga accgtttagg agcaggccgc
     9121 gctcgcgccc gcgctctacc ttacccaggg gtcgaaccgt aagattcgat gaccgacgaa
     9181 cccaacaagc cgcccgacga cgcggccggc gacacgccgg agcggacgga cgcacgcccc
     9241 ggcgcgccgc ggacggacga caagtcgccc agccccggag atgaggtcga acgcgcgggc
     9301 acgctcccgc cgccgcccga cgccgggggg ccgcgcccga cgcccgagag tcaggccgcg
     9361 ctgacgggta aggctccctc gggcagctcc gtcgccggca cgaccgtccc ggccgcctca
     9421 gtcggcgcgg acgagactgt ggggagtgtt tcagcaggga agtcggaagc gacgacgagc
     9481 gcggagacgc ccgagcagaa gaaggcgcga ctcatcgccg agcacaaggc gaagctcgcc
     9541 gcgaaggccg ctgctgctgc cgaaggcgcg gggacagaag ttaataagga cgtgccgggc
     9601 ggggcgtcgc ccgccgtcgg cgaccccacg cagagtccgt cacagtccga tcttgagaag
     9661 gccgcgaaga tcgccgaggc aaaggcccgc gccgcggccg cgaaggaggc gggtgcggcg
     9721 gcagcaggtg cggcggcggg cgcgccgaag gcgccggtca agaagaagga cgaggggccg
     9781 aagcccgtag acgcctctga caacccgctc gtccgtcgcc tgcgcgaacg actcggcgac
     9841 ggcctcctcg gggcggagac cttcctcaac cagctctcga tccgcgtcga gcgcgcgcac
     9901 aacgtcgaaa cctgtcgcgc gcttcgtgat gacgccgaga cgcccttcga ctatttgagc
     9961 gacgtgacct gcgtccactg gcccgaaaac ttcgacgcgc cgttcgagat cgtctacaac
    10021 ctctattcga taaacgggaa cgagcgcgtg cgcgtgaagg tgaacgcgga cgccgagggc
    10081 ggcgtcgaga gcatgacggg cgtgtggccg gcggcgaact ggatggaacg cgaagtgttc
    10141 gacctctacg gcgtgcaatt ccagaatcac cccgacctgc gccgcctgct gctgccgccc
    10201 gactgggagg ggcacccgct ccgcaaggac ttcccgctcg aattcatgga gaacaac
//
LOCUS       DASVES010000020         9412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_756438, whole genome shotgun sequence.
ACCESSION   DASVES010000020 DASVES010000000
VERSION     DASVES010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756438"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VF240_00815"
     CDS             complement(<1..806)
                     /locus_tag="VF240_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7173810.1"
                     /translation="MKNCLDEGMLQSYLDGELSPELSARAATHIGACDACADALGVAG
                     EELALFARSFAPDASLSVPTEALRGRLDAAIAGIESPRTTQRKGTFWNFGALAAWLAA
                     PFNFEPRMAGAFASIVALVALAAIFGSIYFGGGESRQPVGVAQNTPAASAPSPVAPSV
                     TPSVVENPPAPVVTNDKTMTVATNDVKRSRRNRAAGVNNSVRVPKDAREDVAPAGEVA
                     VVQQSVPGEENYLRTIASLSKVVELGGPDVLRPQVRASYERNIVVIDKAIV"
     gene            complement(803..1354)
                     /locus_tag="VF240_00820"
     CDS             complement(803..1354)
                     /locus_tag="VF240_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7173811.1"
                     /translation="MVNSVPETFETAKPSPTFDEAFTLHHRSVYRAAYALVRDTGLAE
                     DVTQEVFIKLYSHLDTLKDEEHVRAWLLRVASNTALNTIRSRGRAAVREEEFVKAAVV
                     ADGVTVRIDSDYERQTELDEARRALEQVKEPMRSCLLLKQQGLSYKEIATTLSVNEAN
                     VGSLVSRGRKEFMRCYGKIGGRR"
     gene            1712..2836
                     /gene="hemE"
                     /locus_tag="VF240_00825"
     CDS             1712..2836
                     /gene="hemE"
                     /locus_tag="VF240_00825"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX7173812.1"
                     /translation="MSEQGISDEAGRSHNVTEMPVEPSPGPVLLTEELRDSPFMRACR
                     REPVPYTPVWLMRQAGRYMPEYREVRARHTFLELCRKPEAAAEVTVYAAHRLNVDAAI
                     IFADILLIVEPLGLQLEFAKGEGPVIHNPVRERADLARLREFEDAGALEYVYEAIRIT
                     RRELHPRLPLIGFCGAPFTLASYMIEGGGSRNYVHTKRLMHADPGAWHALLSLISRAL
                     PLYLNAQTAAGAQVIQLFDSWVGALSPDEYREFVLPHTRSVVRAVTPGVPVIHFGTGT
                     ATLLELMREAGGDVIGLDWRVPLDEGWRRLGDVAVMGNFDQTFLFTTPEIIRREAAKI
                     LERAGGRAGHVFNLGHGILPETPVENVVALVEAVHELSAR"
     gene            2970..4007
                     /locus_tag="VF240_00830"
     CDS             2970..4007
                     /locus_tag="VF240_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7173813.1"
                     /translation="MDKQSYDAVLVVSFGGPEGMKDVLPFLENVLRGRNVPPERMREV
                     AHHYELFGGVSPINEQCRQLIDALREQLRESGPRLPVYWGNRNWHPFLADTLRQMRDD
                     GVGRALAFVTSAYSSYSGCRQYQEDIERARAHVGAGAPAVEKLRVFYNHPGFVAPNAE
                     NLRAALEQIPVERRALARVAFTAHSIPASMASNCDYEAQLLETARLVAEGAGRESWRL
                     VYQSRSGAPTQPWLGPDVCEHLRELKSEGARDVVVAPIGFISDHMEVLYDLDTEARQL
                     AEEIGLNMVRAATVGTHPAFVQMIRALVLERIDAGAPRLALGENGPRADTCAPGCCPV
                     AARPNTLTQAR"
     gene            4015..5493
                     /gene="hemG"
                     /locus_tag="VF240_00835"
     CDS             4015..5493
                     /gene="hemG"
                     /locus_tag="VF240_00835"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX7173814.1"
                     /translation="MSEERTGGRRRVVVIGAGVSGLAAAHRLVETCGAAQLEVVLLEA
                     SARPGGTLRTERRDGFLLEGGPDAFISEKPAALDLARRIGLGPRLIETNEAHRKSFVA
                     RGNRLHPTPEGFQLLAPSSLWPFVTSGIFSWRGKARMSLDLFLPRRRARDAESDESLA
                     DFVRRRLGREALERMAQPMIGGIYTADPELLSLRATMPRFLEMERAHRSLILALRRAA
                     RARTSPGRPRRRGDASNASGARYSLFLSFDSGMQTLTDALAARLPADALRLDTRAAAL
                     SFDPQARQWIVRTHASEEMRADGIIVALPAHSAARLLRETDAVLAAELEAIPYASTAT
                     VNIAYRQEDVPHPLDGFGFVVPASERRATLACSFSSVKFKGRAPGGCVLLRAFVGGAL
                     QPATFELDDERMTQAVRRDLRELLGVEQAPLFTRVERWPRSMPQYHLGHLARVARVRA
                     SLRNFPALRLCGNAYEGAGVPDCVRGGEQAADELAASLRASG"
     gene            complement(5490..6962)
                     /locus_tag="VF240_00840"
     CDS             complement(5490..6962)
                     /locus_tag="VF240_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7173815.1"
                     /translation="MIWLRRMTEGWRGAALGTLVVLGGAATAAVTLWARRAGDWELAR
                     MGAIVSLVFVLLMLLFVVPPLARSARLEAARLDLPIQLTGGGLIFLSILALVAFAAWN
                     TGNNLLFLIFSLMTSAVFVGWSAGRASLRDLVVTARFPDHIFAGEPAPAIITVQNVKR
                     VFPSVSVLVEARSRLDSPVPPRRRRGLAFRRSRFEKRSLGYFMYVPRRSRAEQKVEQT
                     FARRGQVLVTGFELSTGFPFGFFRLRRRLRARDVEIVVYPKPRPASDELHLLPINAGR
                     MASARRGAGQDLHSLRDYQPHDDVRHIDWKATARASRLTVREFAAEDERRVHVVLDTL
                     AGEEPRAAKKDKVGVLGRRRRAGVEASGGVGAAEMTTGGEAEASAVGAAERFERGVTL
                     AASLVAHFVEERAEVRLTLGAEGGVYGSGREHMHACLRRLALVAPDMGAHPAAGREGF
                     WETITPAPAAAADSYVLLLTTAPPGSIPAQVWRASHVIYL"
     gene            complement(6971..7348)
                     /locus_tag="VF240_00845"
     CDS             complement(6971..7348)
                     /locus_tag="VF240_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173816.1"
                     /translation="MSSTDAQDMRPAPVKPAVGIDALERLDVRVGTIELVEDVKGSDK
                     LLKLTVNFGDHRRTILAGMKRERENPKEIEGRQALFVVNLEPRRMAGVVSEGMLFDIG
                     YADGLRPVLAVPEGRVPDGARAG"
     gene            complement(7377..8531)
                     /locus_tag="VF240_00850"
     CDS             complement(7377..8531)
                     /locus_tag="VF240_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7173817.1"
                     /translation="MSTKTFDAAVVGAGVFGAWTALSLVRAGARVVILDAHGPANSRA
                     SSGGESRVIRAGYGAQEFYTRWALRSLRIWRELFDAGGCPELLRQTGVLWLTSERDAL
                     VTDTLSTLRAVGVPVEQLTRAGLERRYPQFDFGAVTWGLYEPEAGVLLARRAVRKVFE
                     ETLKGGGEYLRAAVAAPTGGGRLDGLSLPDGARVSAGAYVFACGPWLPKLFPELLGER
                     IHATRQEVFFFGTPPGDARFSPPLMPVWIDFGAEVYGIPDVDERGFKLALDRHGPAFD
                     PEEDSRLPSAEALAEVRRVLDARFPAMRGAPLVESRVCQYENTSNGDFLIDRHPDFDN
                     VWLVGGGSGHGFKHGPALGEFVAELIDGRRETEPRFSLAAKEKFRRRAIY"
     gene            complement(8568..9098)
                     /locus_tag="VF240_00855"
     CDS             complement(8568..9098)
                     /locus_tag="VF240_00855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7173818.1"
                     /translation="MKKFILLLALAVCCVHAGAGEADASRAGRRGGGVAQKVGKGVRS
                     GAKGAPRVEYLKNVGGTRQLPFSEAVRVGDVLYLSGQIGVDAGGAVVAGGIEAETRRT
                     LENIRTTLERHGSSMDNVFKCTVMLADIRTDYAPMNGVYRTFFRPERMPARSTFGANG
                     LALGARVEIECLATAR"
BASE COUNT         1561 a         3343 c         3012 g         1496 t
ORIGIN      
        1 acgatggcct tgtcgatgac gacgatgttg cgctcatagg aggcgcgcac ctgcggccgg
       61 agcacgtcgg ggccgccgag ttcgaccacc ttcgagagcg acgcgatggt ccgcagatag
      121 ttctcttcac cgggcactga ctgctgcaca acagcgacct caccggcggg cgcgacatct
      181 tccctcgcat ctttcggcac acgaacagag ttgttcacgc cggcggccct gttacgcctc
      241 gaccgcttca catcgttcgt ggccaccgtc atcgtcttat cattcgtgac aaccggcgcc
      301 ggaggatttt cgacgactga cggagtcaca cttggggcca caggggacgg cgcggaggcc
      361 gcgggagtgt tttgggccac gcccacgggc tgccggctct cgccgccgcc gaagtagatg
      421 ctcccgaaga ttgccgcgag ggcgacgagg gcgacgatgc tcgcaaacgc gccggccatg
      481 cgcggctcga agttgaaagg agcggcgagc cacgcggcga gcgcgccgaa gttccagaag
      541 gttcccttgc gctgcgtggt gcgcggggat tcgatgccgg cgatggcggc gtcgagacgg
      601 ccgcgcaggg cctcggtcgg gacactcaga gacgcgtcgg gtgcaaagga gcgtgcgaag
      661 agcgcaagct cttcaccggc tacgccgagc gcgtcggcgc aggcgtcgca cgcaccgatg
      721 tgcgtggccg cgcgggccga aagctcaggt gagagttcgc cgtccagata ggactgcaac
      781 attccctcgt ctaaacaatt cttcatcgac gccctccgat cttcccgtag cacctcataa
      841 actccttgcg accccttgag accagactcc cgacgttcgc ttcattaacc gaaagcgtcg
      901 tcgcaatctc cttgtagctt agtccctgct gcttgagcag gaggcaactc cgcatcggct
      961 ccttcacctg ttcgagcgcc cggcgcgctt cgtccaactc ggtctgccgc tcgtagtcgg
     1021 aatcgatccg cacggtgacg ccgtcggcca cgaccgccgc cttgacgaac tcttcttcgc
     1081 gcacggccgc gcggccgcgg ctgcgaatcg tgttgagcgc ggtgttcgac gccacccgca
     1141 ggagccacgc gcgaacgtgc tcctcgtctt tcagtgtgtc gagatgactg tagagcttga
     1201 taaagacctc ctgcgtcacg tcttcggcca gtcctgtatc acggacgagc gcataggcgg
     1261 cgcggtagac ggagcggtgg tgcagcgtga aagcctcgtc gaaagtcggc gagggcttcg
     1321 ctgtctcgaa cgtctccggc acagagttaa ccaagactct tccagcttgt tcgctcatca
     1381 tcgaataatg cctgcaaggc gccgcgggcg agcgcgccgc gtccgcgccc gctggggcgc
     1441 atcgtgcctg ccccgacacc aaccatctca aaaacccgcg agaaaccgct cactgccccg
     1501 gccgtttgcg cgcttccata gtctaaacac catgaacaca agatttgtgg cagaaaaaat
     1561 cgcgcgcgcc acccgccccg gaagcccgca aatccgcccg ccccgcaccc ggcgtatttg
     1621 gggccggacg caagcgccgc cgctaccgtt tgccggcccc gtattgtagt ctgtttacat
     1681 cactgcactg aagcgacaca gtagatatag tatgagcgaa caagggattt cagacgaggc
     1741 gggacgcagc cacaacgtca cagagatgcc cgtcgagcct tcacccggcc ccgttcttct
     1801 gacggaggaa ctccgcgaca gccccttcat gcgtgcctgc cggcgcgagc ctgtccccta
     1861 cacgcccgtg tggctgatgc ggcaggccgg ccgctacatg ccggagtatc gggaggtgcg
     1921 cgcgcgccac accttccttg agctgtgccg gaagccggag gcggccgccg aggtgacggt
     1981 ctacgccgcg caccgtctga acgtggacgc ggcgatcatc ttcgccgaca tcctcctcat
     2041 cgtcgagcct ctgggcctcc agcttgagtt cgcgaagggc gaaggccccg tcatccataa
     2101 ccccgtgcgc gaacgggccg acctcgcgcg cctccgcgaa tttgaagacg cgggcgcgct
     2161 cgaatacgtc tacgaggcca tccgcatcac gcgccgcgag ctgcaccccc gtctgccact
     2221 catcggcttc tgcggcgctc cgttcacgct cgcctcttac atgatcgagg gcggcggctc
     2281 gcgcaactac gtccacacga agcggctgat gcacgccgac cccggcgcgt ggcacgccct
     2341 gctctcgctc atctcccgcg ccctcccgct ctacctcaac gcgcaaacgg ccgccggcgc
     2401 gcaagtcatc cagctcttcg actcgtgggt cggcgcgctc tcgcccgacg agtatcggga
     2461 gtttgtcctc ccgcacacgc gaagcgtcgt ccgcgccgtc acccccggcg tccccgtcat
     2521 ccactttggc acggggacgg ccaccctgct tgagttgatg cgcgaagccg gcggcgacgt
     2581 gatcggcctc gactggcgcg tgccgctcga cgaaggctgg cgtcgtctcg gcgacgtggc
     2641 cgtgatgggc aacttcgacc agacgttttt gttcacgacg cccgaaatca tccgccgcga
     2701 ggccgcgaag attctcgaac gcgcgggcgg acgcgccggc cacgtcttca acctcggcca
     2761 cggcatcctg cccgagacgc ccgtcgagaa cgtcgtcgcg ctcgtcgagg cggttcacga
     2821 gttgagcgcc cgttgagttt ttagagatgg agaaatagac aggattgaca ggactgacag
     2881 gatgaagaaa gagctgattt gagatttcca atcttcacat cctgtcagtc ctgttaatcc
     2941 tgtctatttc ttcacggatt attgatcgca tggataagca atcttacgac gcggttctgg
     3001 tcgtctcctt cggcggcccc gaggggatga aagacgtgtt gccgtttctc gaaaacgtct
     3061 tgcgcgggag gaacgtgccg cccgagagga tgcgcgaggt ggcgcatcac tacgagttgt
     3121 tcggcggcgt cagcccgatc aatgaacagt gccggcaatt gatcgacgcg ctgcgcgagc
     3181 aactacggga gtcggggccg cgcctgccgg tctactgggg caatcgcaac tggcatccct
     3241 tcctcgccga cacgttgcgc cagatgcgcg acgacggcgt cggccgcgcc ctcgccttcg
     3301 tcacctcggc ctatagctcc tactcgggct gccggcagta tcaagaagac atcgagcggg
     3361 cgcgcgccca cgtcggcgcg ggtgcgcccg cggttgagaa gttgcgcgtc ttctacaatc
     3421 atcccggctt cgtcgcgccg aacgccgaga acctgcgcgc cgcgctcgaa cagattcccg
     3481 tggagagacg cgcgctggcg cgcgtcgcct tcaccgccca cagcatcccc gcgtcgatgg
     3541 cgtccaactg cgattacgag gcgcagcttc tggagacggc gaggctggtg gccgaaggcg
     3601 ctggacgtga gagctggcgt ctcgtctacc agagccgcag tggagcgccg acgcagccgt
     3661 ggctcggccc agacgtttgc gaacacctga gagagttgaa gtcagagggc gcgcgtgacg
     3721 tggtcgtcgc gcccatcggc ttcatctccg accacatgga ggtgctctac gacctcgaca
     3781 cggaggcgcg gcaactcgcc gaagaaatcg gcctcaacat ggtgcgcgcc gcgaccgtgg
     3841 gcacacaccc ggccttcgtc cagatgatcc gcgcactcgt ccttgagcga atcgatgcgg
     3901 gcgcgccccg cctcgcgctg ggcgaaaacg gcccacgcgc cgacacttgc gcgccggggt
     3961 gttgcccagt ggccgcgcgc ccgaacacac tgacgcaggc gcgttgaaca agtaatgagt
     4021 gaggagagaa caggcggcag gcggcgcgtc gtcgtcatcg gcgccggcgt cagcgggctg
     4081 gccgccgcgc accgtctcgt ggagacgtgc ggggccgctc agttggaagt tgtgttgctc
     4141 gaagcgtccg cccggcccgg cggcacactc cgcacggagc ggcgcgacgg ctttctcctc
     4201 gaaggcggcc ccgacgcctt catctccgag aagcccgcgg cactcgacct cgcccgccgc
     4261 atcggactcg gcccacgtct catcgagact aacgaggctc accgcaaaag cttcgtcgcc
     4321 cgtggcaatc gactccaccc gacgcccgaa gggttccagc tcctcgcgcc ctcaagtctc
     4381 tggccgttcg tcacctcggg aattttttcg tggcggggaa aggccagaat gtccctcgac
     4441 ctcttcctcc cgcgtcgccg cgcacgcgat gcggaatccg atgagagcct cgccgacttc
     4501 gtccgccgcc gcctcgggcg cgaggcgctg gagcgcatgg cgcagccgat gatcggcggc
     4561 atctacacgg ccgaccccga actcctctcc ctgcgcgcga ccatgccgcg cttcctcgaa
     4621 atggaacgcg cacaccgcag tcttatcctc gccctgcgcc gcgccgcgcg cgcgcgaacg
     4681 tcgccgggcc gcccccgtcg gcgcggcgac gcgagtaacg cgagcggcgc tcgctacagt
     4741 ctcttcctct ccttcgactc cggcatgcag acgctcaccg acgccctcgc cgcgcgtctc
     4801 cccgccgacg cactccggct cgacacgcga gccgccgccc tttcattcga cccgcaagcg
     4861 cggcagtgga tagtccgcac gcacgcgagc gaggagatgc gcgcggacgg catcatcgtc
     4921 gccctgccgg cccactcggc cgcgcgactg ctgcgcgaga cagatgcggt gctggccgcg
     4981 gagcttgagg cgatacccta cgcctcgacg gcgaccgtca acatcgccta ccgacaggaa
     5041 gacgtgccgc acccgctcga cggcttcggc ttcgtcgtcc ccgcctccga gcgccgcgcc
     5101 actctcgcct gttcgttcag cagcgtgaag ttcaaagggc gcgcgcccgg aggatgtgtc
     5161 ctgcttcgcg ccttcgtcgg cggtgcgctt cagcccgcga cgttcgagct ggacgacgag
     5221 cgcatgacgc aggccgtgcg gcgcgacctg cgtgaactgc tcggcgtcga gcaagcgccg
     5281 ctcttcacgc gtgtcgagcg ttggccgcgc tcgatgccgc agtatcacct cgggcacctc
     5341 gcgcgcgtcg cgcgcgtccg cgcgtcgctt cgcaacttcc ctgccctgcg tctctgcggc
     5401 aacgcctacg aaggcgcggg cgtccccgac tgcgtgcgcg gcggagagca ggcggccgac
     5461 gaactcgcgg cgtcgctgcg tgcgtctggt taaaggtaga tgacgtggga ggcgcgccag
     5521 acctgcgcgg gaatagagcc gggcggcgcg gtcgtcagga ggaggacgta gctgtcggcg
     5581 gctgccgccg gagcgggtgt gatggtctcc cagaacccct cgcggcccgc cgccggatgc
     5641 gcgcccatgt ccggcgcgac gagggccaga cgcctcaggc aagcgtgcat gtgctcgcgg
     5701 cccgagccgt atacgccgcc ctccgcgccg agggtgagac ggacttcggc gcgctcttcg
     5761 acgaagtgtg cgacgagcga ggccgcgagc gtcaccccgc gctcgaaacg ctcggccgcg
     5821 ccgacggccg acgcctccgc ctcaccgcct gtcgtcattt ccgccgctcc cacgcctcca
     5881 ctcgcttcga cccccgccct ccggcgtctt cccaacactc ccactttatc tttcttcgcg
     5941 gcccgaggtt cctcaccggc cagcgtgtcg agcacgacgt gtacacgtcg ctcgtcctcg
     6001 gcggcgaact cgcgcacggt gaggcgggag gcgcgcgccg tcgccttcca gtcgatgtgt
     6061 cgcacgtcgt cgtgcggctg gtagtcgcgc agggagtgga ggtcctgccc cgcgccgcgg
     6121 cgcgccgagg ccatgcgccc ggcgttgatg gggagcaggt gcagctcgtc gctcgccggc
     6181 cgcggcttcg ggtagacgac gatctccacg tcgcgcgcgc gcaagcgccg acgcagtcgg
     6241 aagaagccga agggaaatcc ggtggacaac tcgaagccgg tgacgagcac ctgtccgcgc
     6301 cgcgcaaagg tctgctcgac tttctgctcg gcgcgcgagc ggcgggggac gtacatgaag
     6361 taaccgagag agcgtttctc aaagcggctc cggcgaaagg ccaatccgcg acgccggcgc
     6421 ggcgggacgg gagagtcgag gcggctgcgg gcctcgacca gcaccgagac ggacggaaag
     6481 acgcgcttca cattctgcac cgtgatgatg gccggggccg gctcgcccgc gaagatgtga
     6541 tcgggaaagc gcgccgtgac gacgaggtcg cgcagagagg cgcggcccgc cgaccacccg
     6601 acgaagacgg ccgaggtcat tagcgagaag atcaggaaca gaagattgtt ccccgtgttc
     6661 cacgcggcga aggcgacgag cgcgaggatg gagagaaaga tgagaccgcc gcccgtgagc
     6721 tggatgggca agtcgagccg cgccgcttcg agtcgggccg agcgcgccag cggcggcacg
     6781 acgaagagga gcatcagcag gacgaagacg agcgaaacga tggcgcccat ccgcgcaagc
     6841 tcccagtcgc ccgccctgcg cgcccagagc gtgacggccg cggtcgccgc gccgcccaac
     6901 acgacgagcg tccccagcgc cgccccgcgc caaccttcag tcatccgacg caaccagatc
     6961 atctctcgac tcacccggcg cgcgccccgt cgggcacgcg cccttcgggg acggcgagca
     7021 ccggcctcag cccgtcggcg tagccgatgt cgaatagcat cccttcggac accacgcccg
     7081 ccatccgccg cggctcaagg ttgacgacga aaagggcttg ccggccctcg atctctttcg
     7141 ggttctcgcg ctcgcgcttc atcccggcga ggatcgtccg gcgatggtcg ccgaagttca
     7201 ccgtcagttt caggagcttg tccgagcctt tcacgtcttc caccagctca atcgtcccga
     7261 cgcgcacgtc gagacgttcg agcgcgtcga tgccgacggc gggcttgacc ggcgccggcc
     7321 tcatgtcttg tgcgtccgtt gaactcacag ccttactccg tcccgcgctc agtctctcag
     7381 tagatggccc gccgccggaa cttttctttg gcggcgagcg aaaatctcgg ctcggtctcg
     7441 cgccggccgt cgatcaactc cgcgacgaac tcgccgagcg ccgggccgtg cttgaagccg
     7501 tggccggagc cgccgccgac gagccagacg ttgtcgaagt cgggatgacg gtcgatgaga
     7561 aagtcgccgt tcgacgtgtt ctcatactgg cagacgcgcg actcgacaag cggcgctccg
     7621 cgcatcgcgg ggaaacgcgc gtcgagcacg cgccgcacct ccgcgagcgc ttccgccgat
     7681 ggcagtctcg aatcctcctc ggggtcgaac gcggggccgt ggcggtcgag cgccagcttg
     7741 aagccgcgct cgtccacgtc cgggatgccg tagacctccg cgccgaaatc tatccacacc
     7801 ggcatcagcg gcggcgagaa gcgcgcgtcg cccggcggcg tgccgaagaa gaagacctcc
     7861 tgccgcgtcg cgtgaatgcg ctcgccaagg agttcgggga agagtttggg cagccacggc
     7921 ccacacgcga agacgtaagc gccggcgctc acgcgcgccc cgtccgggag actcagcccg
     7981 tcaagcctcc cgccgcccgt gggcgcggcg acggccgccc gcaggtattc gccgccgcct
     8041 ttcaacgtct cttcaaaaac cttgcgcacc gcgcgacgcg ccagcagcac gcccgcctcc
     8101 ggctcgtaga ggccccacgt caccgcgccg aaatcaaact gcgggtagcg acgctcaagc
     8161 cccgcgcgcg tgagttgttc gacgggcacg ccgaccgctc gcagcgtcga gagtgtgtcc
     8221 gtgacgagcg cgtcgcgttc gctggtgagc cagagcacgc ccgtctgtcg cagcagttcg
     8281 gggcagcccc ccgcgtcgaa caactcacgc cagatgcgta gcgagcgcaa cgcccagcgc
     8341 gtgtagaact cctgcgctcc gtagccggcg cggatgacgc gcgactcgcc gcccgagctg
     8401 gcgcggctgt tcgcggggcc gtgcgcgtcc aaaatgacga cgcgtgcgcc cgcgcgcacg
     8461 agactgagcg ccgtccacgc gccgaacacg ccggcgccga cgaccgccgc gtcaaacgtc
     8521 ttcgtactca agcccacgtc cttctactca actccacgcc gaagcactca acgcgccgtc
     8581 gctaaacatt cgatctccac gcgcgcgccg agggcgaggc cgttcgcgcc gaaggtgctg
     8641 cgcgccggca tccgctcggg gcggaagaac gtgcgataga cgccgttcat cggcgcgtag
     8701 tcggtgcgga tgtcggcgag catcacggta cacttgaaga cgttgtccat cgacgagccg
     8761 tgacgctcaa gcgtcgttcg gatgttctca agcgtccgac gcgtctcggc ctcgatgccg
     8821 ccggccacga ccgcgccgcc cgcgtccacg ccgatctgcc cggacaggta gagcacgtcg
     8881 ccgacacgca ccgcctccga gaacggcaac tgtcgcgtcc cgcccacgtt cttcagatac
     8941 tcaacgcggg gcgcgccctt cgcgccagac ctgacgccct tgccgacttt ctgcgcgaca
     9001 ccgccgcccc gtcgccccgc ccgactcgcg tccgcttcgc ccgcgcccgc atgcacacag
     9061 caaacggcga gcgccaacag aaggataaac ttcttcatct ttcacctctc aatacggtcg
     9121 attcttcgga gcatgcgggc ggaacagtac cgcgagcagt agcgagcggg tctggccgga
     9181 cgatgcgggt ctctcatcac aggcgtaccc gctcgctacc gctcgcggta ctgtcacccg
     9241 ttacgggcgc ggctcgtcgc cgggcgcgct acgccgggct atctcatcat cccttcgggg
     9301 cttctcaaac agcttcttag agcgggacgc tgacggcctt catgatctcg cggagggcgg
     9361 cctcggcttc gtcgctgcgg cggtggaagg aggacgagaa gcgggagttg ac
//
LOCUS       DASVES010000021         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1726901, whole genome shotgun sequence.
ACCESSION   DASVES010000021 DASVES010000000
VERSION     DASVES010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726901"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VF240_00860"
     CDS             <1..282
                     /locus_tag="VF240_00860"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX7173819.1"
                     /translation="EATSRGQMLAAMTDVEGSAGYVAIERVKGTLDGREGTFALQHSG
                     VMTRGVPQLTITVVPDSGTGQLNGLAGAMTIDIAEGKHSYGFEYTLAEG"
     gene            449..2470
                     /locus_tag="VF240_00865"
     CDS             449..2470
                     /locus_tag="VF240_00865"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX7173820.1"
                     /translation="MPTFASRTAARLHGASLLVLAALVAAACAPGAVLQARAQQQPSQ
                     QGWAWQNPLPQGNAINAVRFAADKLSGWAVGASGVILRTRDGGFRWDEQHSGTASTLY
                     GLYVKDRKTAVAVGSRGVVLVTRDGGRKWQPRPTAVADHLYGVAFAPDAAHGWAVGSY
                     GQIVHTPDGGRTWRAQISGTRAHLSSVAFADARRGVAAGDGGALLVTEDGGATWNNQS
                     IKDGAPFTSVAVAGASGRAVAVGLGGLVMRTDDGGKTWARVEVYSRADLLSVYFKDEL
                     HGWISGDEGQVLTTSNGGELWFPVSVKTRPRLSALHFADELTGWAAGRDGLILRTDDG
                     GLVWRRLTEGGRDDVSDIFFLDGERGWAVGARGRILHTSSGGKRWSPMYSGVTANLNG
                     VYFIDRARGFAVGDAGTLLRTADGGISWEKAVTEASADLFTVHFGDDKHGIAAGARGQ
                     LLHTDDSGVTWHLTATGSTSWFESVAHTGAKTAVAVGSHGTVIRTEDGGNTWQFVELN
                     AKEWLYSVSFSDAARGWAVGQKGLVLRTDDGGKTWKDQESGVRHNLYSVAAAEREEAM
                     IVGEQGRVLHTRDGGETWELQPTLTTASLFGVAFRGGDSAWVAGRGGAILRRTDTLAT
                     VRLPRPRLPPALRGGPPKLQPQDQADVAPLIDDDIPRALPPRPKATPKP"
BASE COUNT          438 a          997 c          879 g          389 t
ORIGIN      
        1 gaagccacga gccgcggcca gatgcttgcg gccatgacgg acgtggaagg ctcggccggc
       61 tatgtcgcca tcgagcgggt caaggggacg ctcgacggcc gcgaaggcac cttcgcactc
      121 cagcacagcg gcgtcatgac gcgcggcgtc ccgcagctca ccatcacagt cgtcccggac
      181 tccggcaccg gccagctcaa cgggcttgcc ggcgcaatga cgattgacat cgccgaggga
      241 aagcactcct acggcttcga gtacacgctc gcggaggggt gatgcgggcg ccgccccatt
      301 cggggccgtc aacaagacag ttaccgcccg gatttactcc actctccgca accgattata
      361 atcatcctcg aataatcgac accccgacac tgtttcagca agcaacccga cacaggtcag
      421 gaaccgtcta ccctaactct taagcgttat gccgactttc gcctcccgca ccgccgcacg
      481 cctccacggc gcgagcctcc ttgtgctcgc ggcgctggtc gccgcggcgt gcgcgccggg
      541 ggccgtgttg caggcgcggg cgcagcaaca gccttcgcag cagggctggg cgtggcagaa
      601 ccctctgccg cagggcaacg ccatcaacgc cgtgcgcttc gccgccgaca agttgagcgg
      661 ctgggccgtc ggcgcaagcg gcgtcatcct gcgcacgcgt gacggcggct tccgctggga
      721 cgagcaacac tcgggcaccg cctccacgct ctacggcctc tacgtcaaag accgcaagac
      781 ggcagtcgcc gtcggctcgc gcggcgtcgt cctcgttacc cgtgacggcg ggcgcaagtg
      841 gcaaccgcgt ccgaccgctg tcgccgacca tctctacggc gtggccttcg cgcccgacgc
      901 cgcgcacggc tgggccgtcg gcagttacgg gcagatcgtc cacacccccg acggcggccg
      961 gacgtggcgc gcgcaaatct cggggacgag ggcgcacctc tcttcggtcg ccttcgccga
     1021 cgcgcggcgc ggcgtggccg cgggcgacgg cggcgcgctg ctcgtcaccg aagacggcgg
     1081 cgcgacgtgg aacaaccagt cgatcaaaga cggcgcgccg tttacgagcg tggccgtcgc
     1141 cggcgcatcg ggccgcgccg tcgccgtggg cctgggcggc ctcgtcatgc gcaccgacga
     1201 cggcggcaag acgtgggcgc gcgtcgaggt ctactcgcgc gccgacctcc tctcggtcta
     1261 tttcaaggat gaactccacg gctggatttc cggcgacgag gggcaggtgc tcaccacctc
     1321 gaacggcggc gagctgtggt tccccgtcag cgtcaagacg cgcccgcgtc tctccgcgct
     1381 ccacttcgcc gacgagttga cgggctgggc ggcggggcgc gacggtctca tcctgcgcac
     1441 cgacgacggc ggcctcgtct ggcgtcggct gaccgagggc ggacgcgacg acgtgtccga
     1501 catctttttc ctcgacggcg agcgcggctg ggccgtcggc gcgcgcgggc gcatactcca
     1561 cacctcttcg ggcggcaagc gttggtcgcc gatgtactcg ggcgtcacgg cgaatttgaa
     1621 cggcgtctac ttcatcgacc gcgcgcgcgg cttcgccgtc ggcgacgccg gcacgctctt
     1681 acgcaccgcc gacggcggca tctcttggga gaaggccgtc accgaggcga gcgccgacct
     1741 cttcaccgtc cacttcggcg acgacaagca cggcatcgcc gccggcgcgc gcggccagct
     1801 cctccacacc gacgacagcg gcgtgacgtg gcacctcacg gcgacgggca gcacttcgtg
     1861 gttcgagtcg gtcgcgcaca caggagcgaa aacggccgtc gccgtcggct cacacggcac
     1921 cgtcatccgc accgaggacg gcggcaacac gtggcagttc gtcgaactca atgcgaagga
     1981 gtggctctac tcggtcagct tctccgacgc cgcgcgcggc tgggccgtcg gccagaaggg
     2041 attggtgctg cgcacggacg acggcggcaa gacgtggaag gatcaggagt cgggcgtgcg
     2101 tcacaacctc tactcggtgg cggccgccga gcgtgaggag gcgatgatcg tcggcgagca
     2161 ggggcgcgtg ctccacacgc gcgacggcgg cgagacgtgg gagttgcagc cgacgctgac
     2221 gacggcctcg ctcttcggcg tcgccttccg cggcggcgac tcggcgtggg tggccggccg
     2281 cggcggcgcc atcctccgcc gcaccgacac gctcgccacc gtccgcctcc cccgcccccg
     2341 cctgcccccc gccctgcgcg gcggcccccc caaactccag ccacaggatc aggccgacgt
     2401 cgcccccctc atcgacgacg acatcccccg cgccctcccg ccccgcccca aggccacgcc
     2461 caagccctag ccctccgcca cagcttccgg ccaagaccca cgaaccctta cgacattcac
     2521 gccgggctgt gaaaaattga atttcactga ttagaccgcg caccgcggcc acaggcgaga
     2581 atccacttgt ttatcccgcg ccgtgcggta ttatccggca atgaaactat aaacgcggca
     2641 ccttgcggcc taactgtggt tagcccgcaa tgaccacaag gaagcatcgc ctgaaatgca
     2701 tcg
//
LOCUS       DASVES010000022         4662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_172247, whole genome shotgun sequence.
ACCESSION   DASVES010000022 DASVES010000000
VERSION     DASVES010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172247"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2214)
                     /locus_tag="VF240_00870"
     CDS             complement(<1..2214)
                     /locus_tag="VF240_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173821.1"
                     /translation="MKKYTIFFLVTLSLLSAPLVPTTTLARPAAPQSSALRMRREAPA
                     ANNKKKLKTSPPKTSPPTKAAPAARPLLLRSTQQESSPTPVVARATRFAVSPPVSEMG
                     IDTKETDSMRRGKDFEVERPEKITTPPLSGITDSPEALAASIQSSAPTLNIAAPSGTF
                     EGLSNQDNFNHPAIAGRVLPPDTVGDVGPTQYVQAVNLLFRVFSKTGTPLTPVRPISN
                     LFTALGGRCATTDDGDPIILYDSFADRWIVTQFMVSGAAPLAQCVAVSQTGDATGSYF
                     TYEFVMPNAKFNDYPHFGVWPDGYYFSNNQFNLAGTAFLGVGVFALDRAKLLAGDPAA
                     SYIYFDLETAVPNARSMLPSDADGLTPPPAGAPNVFSYFNANEFVGETDSLRLYNFHA
                     DFSNPAASTFTQRPESPLAVAAFDPRNPAGEDDIEQPPPATTGSALDSISDRLMYRLQ
                     YRNYGTHESLAVNHTVNVGAGTTLATHQAGVRYYELRRTLPAGAWAVNEQATFAPDAT
                     NRWMGSAAMDHQGNLAVGYSASSNTVFPSIRYAGRLASDPPNGLFQGENTIIAGGFSQ
                     TATGSRWGDYSSLNVDPKDDCTFFYTQEYYAADNPATTAEWQTHVGSFKVNPACQAPA
                     QGTLQVNVTDCDSNLPLAGARVSIDGNLYGTSLTGGSFSTQLSPGAYNVSVTLPNYSV
                     GTGTATVTDGGATVIDLCAAPSPFITPAGSALGAESCGPDTDAIDPGETVTVDLTL"
     gene            complement(2952..3527)
                     /locus_tag="VF240_00875"
     CDS             complement(2952..3527)
                     /locus_tag="VF240_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173822.1"
                     /translation="MARNVNTKFAAHAALPFALLVVLLLAQGGSCQKGAAGTNAAKSS
                     NPAAAKASNSVAGANSKAGDGKGVTAVNKMSETTSGGDVAAGSWGGQHVRLEVKSDGA
                     EIEFDCAHGRINGRLRPGADGRFDAAGTFVAERGGPVREGASEKGQPARYTGQIKGKT
                     MTLSVRLTDSDTDAGAFTLTHGRESELVKCR"
     gene            3810..4604
                     /locus_tag="VF240_00880"
     CDS             3810..4604
                     /locus_tag="VF240_00880"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX7173823.1"
                     /translation="MLTDLNDLPSALAMLQGLVHGADPKATMQVVVRWLHFVAGITWI
                     GLLYFFNLVNVNFMKGLDGPTKKIVIPALMPKALWYFRWGAVVTVLVGLMYYAMYILP
                     AEAPAAGLSRGEWMGLLGIWLLVVLVTYAVMFFLYRVPALTKDGRILAVVIAILMVVM
                     TCLVLYVLGSKSNNCDSTGNCVYLGNKTLSIGVGGGMGILMLLNVWGIIWPAQKRIIA
                     GVISGEAAPPALARAAFLASRTNAWLSLPMLFLMAQSHDYAFFAGK"
BASE COUNT          713 a         1393 c         1731 g          825 t
ORIGIN      
        1 cagcgtgagg tcaaccgtaa cggtctcgcc ggggtcgatg gcgtcggtgt cagggccgca
       61 gctctcggcg ccgagcgcgg agccggcggg cgtgatgaag ggcgacggcg cggcgcagag
      121 gtcgatgacc gtggcgccgc cgtcggtgac ggtggcggtg cctgtgccga ccgaatagtt
      181 ggggagtgtg accgagacgt tgtaggcgcc gggcgacagt tgggtggaga aggagccgcc
      241 cgtcaggctc gtgccgtaga ggttgccgtc gatagagacg cgcgcgcccg cgagcgggag
      301 gttcgagtcg cagtcggtga cgttgacctg gagcgtgccc tgcgcggggg cctgacaggc
      361 cgggttgacc ttgaaactgc cgacgtgagt ctgccactcg gcggtcgtcg ccgggttgtc
      421 ggccgcgtag tactcctgcg tgtagaagaa cgtgcagtcg tctttggggt caacgttgag
      481 cgagctgtag tcgccccagc gggagccggt cgccgtctgc gagaagccgc cggcgatgat
      541 ggtgttctca ccctggaaga gaccattcgg cgggtctgag gcgaggcggc cggcgtagcg
      601 gatcgagggg aagaccgtgt tgctggaggc gctgtagccg acggcgaggt tgccctgatg
      661 atccatcgcg gctgagccca tccagcggtt cgtcgcgtcg ggcgcaaagg tcgcctgctc
      721 gttgacggcc cacgcgcccg cgggcagcgt gcgccgcagc tcgtagtagc ggacgccggc
      781 ctggtgcgtc gccagcgtcg tgccggcgcc gacgttgacg gtgtggttga cggccagcga
      841 ctcatgcgtg ccgtagttgc gatactggag gcggtacatg aggcggtcgg agatggagtc
      901 gagggccgag ccggtggtgg ccggcggcgg ctgctcgatg tcgtcctcgc cggccgggtt
      961 gcgcgggtcg aaggcggcga ccgccaaggg gctttcgggc cgctgcgtga aggtcgaggc
     1021 ggccgggttc gagaagtcgg cgtggaagtt gtagagacgg agcgagtcgg tctcgccgac
     1081 gaactcgttg gcgttgaagt aggagaagac gttgggcgcg ccggcgggcg gcggcgtgag
     1141 gccgtcggcg tccgagggca gcatgctgcg cgcgttgggg acggccgtct ccagatcgaa
     1201 gtagatgtag ctggctgcgg ggtcgccggc cagcaacttg gcgcggtcga gggcgaagac
     1261 gccgacgccg aggaaggccg tgccggcgag attgaactga ttgttcgaga agtagtagcc
     1321 gtcgggccag acgccgaagt gcgggtaatc gttaaacttc gcgttcggca tcacgaactc
     1381 gtaggtgaag tacgagcccg tcgcgtcgcc ggtctgcgag acggcgacgc actgcgcgag
     1441 cggcgcggcg cccgagacca tgaactgcgt gacgatccag cggtcggcga acgagtcgta
     1501 gaggatgatg gggtcgccgt cgtcggtcgt cgcgcagcgt ccgccgaggg cggtgaagag
     1561 gttgctgatg gggcgcacgg gcgtgagcgg cgtgcccgtc ttgctgaaga cgcggaagag
     1621 gaggttgacg gcctgcacgt actgcgtcgg ccccacgtcg cccaccgtgt cgggcggcag
     1681 gacgcggccg gcgatggccg ggtggttgaa gttgtcctga ttgctcaggc cctcgaacgt
     1741 gccactcggg gcggcgatgt tgagcgtggg cgcgctcgac tggatggagg cggccagcgc
     1801 ctcgggcgag tcggtgatgc cgctcaaagg cggcgtcgta atcttctcag ggcgctcgac
     1861 ctcgaagtcc ttgccgcgcc gcatgctgtc ggtctctttg gtgtcgatgc ccatctcgct
     1921 gacgggcggg gagacggcga atcgtgtggc gcgcgccacg acgggcgtgg gcgacgactc
     1981 ctgctgcgtg ctcctgagca acagcggccg ggcggcgggc gcggccttcg tcggcgggga
     2041 agtcttcggc ggggaagtct taagcttctt tttgttgttc gcggcgggcg cttcgcggcg
     2101 cattctcagg gcggaagact gtggcgcggc gggccgcgcg agagtggtcg tggggacgag
     2161 cggcgccgaa agcaaagaga gtgtgacgag gaaaaatatt gtgtacttct tcatcgggtc
     2221 ctccggatcg ggtggggagt tgcgtccgcc gggcgggcgc gcggagtctc gaccgagacg
     2281 caacccgcgc cgatgtcctg ctggtcgcgg gcgtcggggg ggggtggtct cgtcatcgag
     2341 ttggaagttg cgacgttgat gtcttcagcg gccgtcggac aggggaatca ttcggggagg
     2401 cagcccgcaa ggccgggcct ttgcacgcgc gaaggcgcgc ggctatctcg ggcacgtgcc
     2461 ccggaatgtc ccctgggaag tcgccgacgg gcggcggata gagccaccgg tcacccggcg
     2521 ggttgtttga ttgctaaggc ggcgaggagt gcgatcctat gaggtgctgg ggcgtttaac
     2581 tttcagaaag tcgggttaaa ggcgtctcgg gacggccggc aaggattggg gcgcgggcgg
     2641 cgccgtccgt gaagatactg cgcgacgcct ttcgaaagcc ctgtcagcgt ttcgtctgcc
     2701 gaaaagtggg tttcggaatg ttcaaaaaaa tggaagtgaa accaacgacc ggtacggccg
     2761 cgcacagtat aattttcgcg ggcgggtgtc aatcaaattc tctgtgaata ctgggcggcg
     2821 cgcggcgggt cgcgggacgg gcgaggcgga aatgaggttg agagggagat gaagatgagt
     2881 aaggacaaag gccgggccac gctacggccg tagagttcgg ggccgcggcg gccgtgtgcg
     2941 tccctcgggc ctcagcggca cttgaccaac tccgactcgc gcccgtgggt cagggtgaag
     3001 gcgccggcat cggtgtccga gtcggtgagg cggacgctga gggtcatggt cttgcctttg
     3061 atttggccgg tgtagcgggc cggctggccc ttttcactcg cgccctcgcg cacggggccg
     3121 ccgcgctcgg cgacgaaggt gcctgcggcg tcgaagcggc cgtcggcgcc gggccgcaga
     3181 cgcccgttga tccgcccgtg cgcgcagtcg aactcgatct cggccccgtc cgactttact
     3241 tcgaggcgga cgtgttggcc gccccaactg ccagcggcca cgtcgccgcc gctcgtcgtc
     3301 tcactcatct tgttcacggc cgtcacaccc ttcccgtcgc cggccttgct gttcgccccg
     3361 gcgacggagt tcgacgcctt cgcggctgcc gggttcgacg acttcgcggc gttcgtcccg
     3421 gccgcgccct tctggcagga gccgccctgc gcgaggagga gcacgacgag gagcgcgaag
     3481 gggagtgcgg cgtgcgcggc gaatttcgtg ttcacgttac gggccaagag aaaagttctc
     3541 cgtcacgggt tgccgaagct gcgtgcgggc acattatgcc ccacgacgac cgggaaggac
     3601 aggattgcca agcgttcggc ttggtgataa tctctcgcgc gtaatcttga attaaacgaa
     3661 agcaccaccg caaccgtccg cgcgcgcgcg tctcgcgtcg ggaaccggat gggcgctccc
     3721 acgtccgccc gtctgtcacc gtcaccgtct ttagcagagc gcgcgcgcgg gcgccggtgg
     3781 ccgtcatctc aaaaacttcg gaggacctca tgctgaccga cttgaatgat ctcccctcgg
     3841 cgctggcgat gctccagggc ctcgtccacg gggccgaccc gaaggcgacc atgcaggtcg
     3901 tcgtccgctg gctccacttc gtcgccggca tcacctggat cgggctgctc tacttcttca
     3961 acctcgtcaa cgtcaacttc atgaagggtc tcgacggccc gacgaagaag atcgtcatcc
     4021 ccgcgctgat gccgaaggcc ctgtggtact tccgctgggg cgccgtcgtc accgtgctcg
     4081 tcgggctgat gtactacgcc atgtacatcc tgccggcgga agcgccggcc gcggggctgt
     4141 cgcgcggcga gtggatgggg ctgctcggca tctggctgct cgtcgtgctc gtcacctacg
     4201 ccgtcatgtt cttcctctat cgcgtcccgg cgcttacgaa agacgggcgc atcctcgccg
     4261 tcgttatcgc catcctgatg gtcgtcatga cgtgcctcgt cctctacgtg ctcggctcga
     4321 agagcaacaa ctgcgactcg accggcaact gtgtctacct cggcaacaag acgctctcga
     4381 ttggcgtcgg cggcgggatg ggcatcctga tgctcctgaa cgtgtggggc atcatctggc
     4441 cggcgcagaa gcgcatcatc gcgggcgtca tctcgggcga ggccgccccg cccgccctcg
     4501 cccgcgccgc cttcctcgcc tcgcgcacca acgcctggct ctcgctcccc atgctcttcc
     4561 tgatggcgca gtcgcacgac tacgccttct tcgcgggcaa gtaatacagg agtcaggagt
     4621 caggagtcag gagtcagaag tcagaatgaa gagagcgaac ct
//
LOCUS       DASVES010000023        19497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1530508, whole genome shotgun sequence.
ACCESSION   DASVES010000023 DASVES010000000
VERSION     DASVES010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19497
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530508"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VF240_00885"
     CDS             <1..1033
                     /locus_tag="VF240_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7173824.1"
                     /translation="IARLELRGDEAKAERGQLFYRELQERVSSLAGVRSVGLTRIVPL
                     SGGGMRRTVYIDGYEPRPGEDTEINSNMISAGYFETMRIPLVAGRDFGTQDVKGAPGV
                     VIVNEEFARRYLRGGSPVGKFLRADSEGPPLEVVGIVRDGKYRNLREDTLPFFYLPLA
                     QNYSPGVALLVRAEGEPTALLQSVRGEIQGLNKNLPVYGINTLEGHLAAALAADRMIA
                     VLLSVFGGAALLLASVGIYGVMSYMVAQRTREIGIRMALGARAGDILGMVVRQGMTLV
                     CVGGVLGLAAALAATRFLRSLLYGVSATDPLTFAGIALLLAAVALLACYLPARRAAKV
                     DPMVALRYE"
     gene            1132..3534
                     /locus_tag="VF240_00890"
     CDS             1132..3534
                     /locus_tag="VF240_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173825.1"
                     /translation="MGTLLQDVRYGLRLLRKRPTFTAVVVCTLALGIGANTAIFSVVY
                     GVLLRPIPYVDGERLIVIETGKKQAGAEQLGGVAPADFWDLQEQSRSFEHVAAISGGG
                     FSLTGVENPEMIPSARVSSGFFGVLGARPALGRAFTKEDELLASPDAVILSHGLWQRR
                     FGGDPSVVGRAFGDTGATIVGVMPPDFKFPVTAECWTPLERDSGEMRNRANSYFNVLG
                     LLAPGQTTDGARAEIETVFARLSAEYPKTNAERAATLTPFREVLVRDVRPSLLVLLGA
                     VGFVLLVACANVANLMLARAAARRREWAVRSALGASRWRLVRQMLTESVMLALMGSAA
                     GLLLAVWGRDVLTAMLPEDFANLQLADQSRIDSTVMLFTLGVALVTGVVFGIFPAWQA
                     TRLKSAEWLKESGRGHVGTSGQRTRSALVVMEVALALVLLVGAGLLLQSFARLRQVDL
                     GFNPHGLSSVGVSTSFAKQPTDGARAQFVAQVLERVSQTPGVESAVVTSGNPFPYLNF
                     NFTVEGRPTDAEAPAVYDSVSPDYFRALGARLVRGREFNAFDRADTTRVALINETLAR
                     RHFAGEDPLGRRVSINYLGQPQLREIVGVTSDINQGEPGKVKPQIYVPYDQQPWLSAS
                     LLVRAGGGDAGATKRAVQEAIWSVDKTQPVAKFESVEQKLGGALAEPRLYTTLLGAFA
                     ALALLLATIGIYGVMSYTVAQRTHEIGVRIALGAQPRDILRMVVGHGLTLTLCGVGAG
                     LLAAFALTRFLQGLLYGVSAGDPVTFALVPLLLSGVALAACYLPARRAMRVDPLAALR
                     EE"
     gene            3679..6114
                     /locus_tag="VF240_00895"
     CDS             3679..6114
                     /locus_tag="VF240_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173826.1"
                     /translation="MNTIWQDMRYGARMLWKSPVFTAVAVLALALGIGANTAIFSVVN
                     ALLLRPLPLAESERLVVLWSQSKKTGALTSSSYPDFADFREQNRVFEGVATLRPRGVT
                     LTGAGEPERLEGARVSADFFPLLRVQPLHGRAFLPEEDRPGGERVVLLGHGLWQRRFG
                     GDPSIVGRSLLLDEQPHTVVGVLPQDFRFPYTLENAEVYSTIAYEGGNLQERGARTSY
                     MLARLREGVTIDEARADAARIAAGLEEQYPDRDTGMGATVVGLQEQLVGKVERALWVL
                     LGAVGLVLLIACSNVANLLLVRATTRHREIAIRTALGASRWRVVRQLLTESLMIAALA
                     GVCGLLLAVWGVDLLVALSPDDLPRLDAVGLDARVLAFTFGVSALTGVLFGLVPALKA
                     SKTELSESLKEGGRSTNMRPGRQRLRGLLVTGEVALALMLLVGAGLLLKSFGRLQAVD
                     PGFDPKGVLTMRINLPRARYTDGESRARFVGEVVERVRELPGVAAAAFVTPVPFSSSN
                     VSSDFAVEGRPDAPVGQEPSASYRGITPDYFRAMNIPLTRGRQFTEQDRRGGPAGVAI
                     INEAVARRYFEGEDPLGKIIKSVGVSVDDDEPERWEIVGVVGNVRHAGLDVEPFAEIY
                     FPHRQQSWNWGHFVVRADGDLAALAPAVRSQVHAIDRDQAISEVRPLTEMIAGTVAKP
                     RFYALLLAAFAVVGLLLAVVGIYGVLSYTVTERTHEIGVRMALGAQRGDILKLVVTGG
                     MKYALAGVVVGLLGARAVTHVLSNLLFEVSATDAPTFAGIALLLAAVALLACYLPARR
                     AAKVDPMVALRYE"
     gene            6214..8652
                     /locus_tag="VF240_00900"
     CDS             6214..8652
                     /locus_tag="VF240_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173827.1"
                     /translation="MGTLLQDTRYGFRMLRKRPGFTAIAILALALGIGANTAIFSVVN
                     AVLLRPLPFAGAERLATVEVRSERTGAVGADHSYPNLVDLREQAGTLEHLAAYSMTTA
                     FLAAGDEPERVRGLLTTADLFPALGVQPLHGRVFTREEDQKGATRVVVLSHGLWQRRF
                     GGDPAIVGKEIPLGGIRSTPTLVLGVMPEGFKFPVGVANVDYWMPLVPILSDSDLSRA
                     SVWLTGLAKLKEGASEEQARSELQTISRRLEEQYKDSNADATFMLRSYHEQLVGDMKT
                     ALLVLLGAVGCVLLIACANVANLMLARAAARGREISIRTALGASRAQIVRQLLTESVL
                     LSILGGALGLLIAWWGVDVLVAAVPSDLPRAAEVALDARVLLFTAALSILTGLIFGLA
                     PALQASRADVSESLKEGGRGSVEGGRSPLRSVLVVAEVAVSLVLLVGAGLLAQSFARL
                     MDVSPGFDAERVLAFDVVFRGENYTQRAATVPALEELLTQAAAVPGVEAVGVVNPLPL
                     GGNFEANSFQIEGRAPAEPGREPSADRRVITSDYFRAMGIHTEGGRVFDSRDAAAATP
                     VAIINQTFARQHFPGENPIGRRLLFTNTQNVTTAREIVGLVADVRHAGLDAESGPEFY
                     VPFAQAPAARVTVVARTTSDDPAQLAPALRGAVRQVDKNFPVYNVRTMESYLAESVAR
                     RRFNMTLLGGFALVALLLAGLGIYGVMSYTVAQRTHEIGVRMALGAQARDVLRLVIRQ
                     GMAPALAGVGVGLVCALALTRVMSSLLFGVTATDPLTFAGVALLLSVVALVSCYLPAR
                     RATKVDPMVALRYE"
     gene            8745..11165
                     /locus_tag="VF240_00905"
     CDS             8745..11165
                     /locus_tag="VF240_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173828.1"
                     /translation="METLLQDVRYGLRTLLKRPGFTVVAVMTLALGIGANAAIFSVVN
                     GVLLRPLPYRDAERIMTVWQRNAQTGAEREPVSPPNFLDFRERNQSFEQLAALRPYGL
                     DYTGAGEPETFQTWRVTEGFFEAAGAVALHGRTFLPEEYRDGVDQVVVIGHGLWQRRF
                     GGDPGVVGQQLVLDGKPRTVVGVMPPEFHYLDKREMIAPYHIDAHELQRRAATYLNVV
                     GRLKPGVTLEQARSDMSNVAARLAEEYPQANGGMGAAVAPLAEQMLGRVRPALWVLFG
                     AVGFVLLIACANVANLMIARSASREREFAIRAALGAGRGRLMRQLLTESLVLAGLACA
                     GGLLLASWGIDAIIALSPAELPRIDQVRLDGRVLLFALGVSAATALFFGLLPALRSSQ
                     PDLQESLKEASRSATAGAARHRLRGFLVVTEFALALVLLVGAGLLVRSFMRLLEVDPG
                     FSAERALTLQVHVYDQYPKPEQQAVFFEQALERLRALPGVEAAGAASAPPFIGEGAIE
                     IDSPIQIEGQPAPPAGQEPTAFHTIVTADYFGALGVPLRGGRIFNQFDRADGAPVVIV
                     NETLARRYWPGESAVGKKLIVLRLSKPVAREVVGVVGDVRHTGLDSEPRPELFLHSLQ
                     NPFGSMTFVVRTTPEPSTLLQAVKGEVWAVNKNLPFYSTATMESLVAETLKERRFSLL
                     LLGAFASLALVLAGVGIYGLISYSTAQRTHEIGVRVALGARGRDIMRMVVGEGLLLSG
                     AGVALGLLGALVLTRFLGGLLYGVAPTDPLTFAGIALLLVCVALAACYLPARRAARVD
                     PMVALRYE"
     gene            11255..13684
                     /locus_tag="VF240_00910"
     CDS             11255..13684
                     /locus_tag="VF240_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173829.1"
                     /translation="MGTLLQDIRYGLRGLWRQPAFTLVAVCSLALGIGANTAIFSLVN
                     AVLLKPLPFADSERLVMVWEDASVIGAPRDSVAPANFVDWKTQNQVFDEMAVLSWQSF
                     NITGDGEPERVTAYAVTANVFPLLGTEPALGRHFLPEENEPGTAKVAMLSHGLWQRRY
                     GGDPSIVGRDIMLNGEKRTVVGVMPQGFQFLQSFIGLWVPTALTQEELANRGAHYLTV
                     LARLKPGVTAEQAQADIGAITDRIAQADPNEAGNLKAAVVPLREQLSGGVRNSLFILL
                     AAVGFVLLIACANIANLLLARATARQREIAVRTALGASRWRVVRQLLTESVLLSVMGG
                     GAGLLLAAWGFEFLKKLVPDAMALSATLKLDLPVLGYALAVSVLTGLVFGLAPALQAS
                     RIDLGEALKQSGGRTGLTAGGGRLRGALVVVEVALALVLLVGAGLLIQTISRLQQQYT
                     VFQPEQLLTLRTVLPEGKYKELSRRVDFYDQVLARVETLPGVVSAGYTTSVPLQWKGG
                     ANGFTMEGPPPEGVLATNAIHRQVSAGYFKTMGVGLREGRYLEERDDAKGQQVMVVNE
                     TMARQYWPGETAIGKRIKFGPAEGTDPWRTIVGVVADVRQMGLDAPVKAEMYLPYRQI
                     ASHAFYKPRDLVVRTSVDPMSLVSAVRGEIHAVDPEQPLSNIATMEKLLADETGARGL
                     GMMLLSTFAGLALLLAALGIYGVLAYFVTQHVPEIGVRLALGARPRDILALVLKKGMG
                     LALGGVAAGLLAAFALTRLMEKLLFGVTATDPATFAGVAALLVAVAFAACYIPARRAT
                     KVDPMVALRYE"
     gene            13771..16236
                     /locus_tag="VF240_00915"
     CDS             13771..16236
                     /locus_tag="VF240_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173830.1"
                     /translation="MGTLWQDLRFGIRILLKKPGFTAVAVLTLALGIGANTAIFSVVN
                     RVLLRPLPFTQPEQLVRVTADIQKTNSTDVGLSGLELFDYRDRAGMFEQISGVYPINA
                     NITEIDQPERVEALLVDVNYFSLLGVEAQLGRTFQQEDYQPGIAEVAVISDGLWRRRY
                     GAGTDVIGKKFRLDEDLYVIAGVAPASFRHPGRTLQTDVEVWVPAGWVGPPFNNPPRG
                     AYFLAGAIGRLKPGVTLPAAQARLAELAGELRREYPNDYPEGGGWTPRLVGLQDDLVG
                     NVRPALLLLLGAVGLVLLIACANVANLLLLRASARQREIAIRRALGAGRIRLIRQLMT
                     ESVLLAVAGGALGLLIAVWGVETLVKFSPANISRLGEIGVDASVLVFTLAVSLVTGVV
                     FGLVPAIQASRPNLQESLKDAARGATAGAARNRMRSVLVISEFALALMLLISAALLIR
                     SFWMLQSVNPGFRAENVLTARLWLPAPNDRKTGPYFTHQSRVQMYKEALQRLAALPGV
                     ERVGGVSSLPLDGGRPTAPFTVEGRPPESANVNTVGFHYASPGYFDAMGIPLLKGRGF
                     AEQEDEKAPGVVLISRTMAERFFPGEEPLGKRVRLGGHNSKAPWLTVVGIVGDVKSER
                     LDAENQPQMYTSLLQNSNLFMAFVIRTANNPAALSEAVRSEIRAVDPDLPVHGVRTME
                     EVMSATVAQQRFAMTLLGVFAVIALLLAAVGIYGVMSYAVAERTHEIGIRMALGAQAG
                     DIVRMVVRQGMILAVVGVVAGAAAALLLTRVMSSMLYGVSASDPVTFAAISLALAAVA
                     FLACYLPARRAAKVDPMVALRYE"
     gene            16330..18777
                     /locus_tag="VF240_00920"
     CDS             16330..18777
                     /locus_tag="VF240_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173831.1"
                     /translation="MRTLLQDLRYGLRVLRKSPGFTLVAVVSLALGIGANTTIFSMAN
                     ALLLRPLPVDEPERLVSLHSVSPDGSSFHTFSYPDYQDYRDQSDVFEGLLVYTIDTYS
                     LNADERTERVFGMLASGNFFDVMGVRPALGRFFTPEEDKAPRGEAVAVLSNGFWQRRF
                     GSDPSIVGRELLFGGQPYTVVGVAPRGFTGTRVGFAPDVYLPMWSHGRSARGSEGLND
                     RSGGSLEIVGRLKRGVSVEQAQAQAGGVAARLAEAYPDSHRGKGVEVRRLGTGLGQFE
                     APVMGFMGVLSAVVGLVLLIACANVAGMALARSATRRKEVAIRLAVGARRSRIVRQLV
                     TESVLLFLIGGAAGVLLAAWLADLLMAFKPPTPFPVELNFGLDWRVLVYALGLSLVTG
                     IVFGLAPALQASRPDVIPSLKDESGGGFRRSRLLNVFVAGQIAVSLVLLVCTGLFLRS
                     LQNARSMDPGFNPDGVQLVGLDLRIQGYDEPRGRRFYAQLLERLKSAPGISSVTLARM
                     IPLDGGNMEYGINVEGREPPDGRRSFSNDTNVVGSDYFKTLGIPLVRGRDFGPQDVKE
                     APRVAVVNEAMARHYWPNETAVGRRFYIGSFADGVPVEIVGVAKDGKYRTLGEEARPY
                     LYLAAEQDYSSYLTLHVRPRSAETAADALAAVRRETAALDPNLPLLDVMPMTEAIGTS
                     LLPIRMAATVAGVLGVVGLVLAGVGIFGVVSFSVVQRTREIGVRMALGAQTRDVLRLV
                     IWQGMKLALAGLAAGLLLAIALSRVLSGLLYGVSPTDVSVFAGVSLLLATAALAASYI
                     PARRAAKVDPMVALRYE"
     gene            18868..>19497
                     /locus_tag="VF240_00925"
     CDS             18868..>19497
                     /locus_tag="VF240_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173832.1"
                     /translation="MDKLLQDLRYGVRTLAKRPGFTAVAVLTLALGIGANTAIFSVVN
                     TVLLNPLPYEGADRLVMLFEQTPAQERNFISRPNLQDYAEQSRTFEEFSAMVGQSVNL
                     TGTEQPDRLIGNFVTSSFFRVLRARPLHGRLFEANEDAEGAERVAILSHPTWQTRFGG
                     DPSIVGRALTLNGEPHTVVGILPEGFRSPGSDYDVYMPAQFWPNYKVARD"
BASE COUNT         2854 a         6494 c         6686 g         3463 t
ORIGIN      
        1 catcgcgcgg ctcgaactcc gcggcgacga ggcgaaggcc gagcgtggac aactcttcta
       61 cagggaattg caggaacgcg tctcgtcgct cgcgggcgtc cgctcggtgg gcctgacgag
      121 gatcgtgccg ctcagcggcg gtggtatgcg ccgcaccgtc tacattgacg gctacgagcc
      181 gcgccccggc gaggacacgg agatcaactc gaacatgatc tccgccggct actttgaaac
      241 gatgcgtatc ccgctcgtcg ccgggcgcga cttcgggacg caggacgtga aaggcgcgcc
      301 gggcgtcgtc atcgtcaacg aggagttcgc gcgccgctac ctgcgcggcg gcagcccagt
      361 cggcaagttc ctgcgcgccg actccgaagg cccgccgctc gaagtcgtcg gcatcgtgcg
      421 cgacggcaag taccgcaacc tgcgcgagga cacgctcccg ttcttctacc tcccgctcgc
      481 tcagaactac tcgccgggcg tggcgctcct ggtgcgtgcc gagggcgagc cgacggcgtt
      541 gctccagtcg gtgcgcggtg agattcaggg gcttaacaaa aacctccccg tctacggcat
      601 caacacgctg gaggggcacc tcgcggcggc gctcgccgcc gaccggatga tcgccgtgct
      661 cctgagcgtc ttcggtgggg cggcgctgct gctggcgtcg gtcggcatct acggcgtgat
      721 gagttacatg gtggcgcagc gcacccgcga gatcggcatc cgcatggcgc tcggcgcgcg
      781 cgccggcgac atcctcggga tggtcgtgcg gcagggcatg acgctcgtct gcgtcggcgg
      841 cgtgttgggt ctggcggcgg cgctcgccgc gactcgcttc ctgcgcagcc tgctctacgg
      901 cgtcagcgcc accgacccgc tgaccttcgc cggcatcgcg ctcctgctcg ccgccgtcgc
      961 tctcctcgcc tgctacctac cggcgcgccg cgcggcgaag gtcgatccga tggtggcact
     1021 caggtacgag tgataagtga tgagtgataa gtgataagtg gaagactgct gtcttctgtt
     1081 tttcttatca ctcatcactt atcactcatc acttctgaaa gaggtttgct catgggaaca
     1141 ctgctccaag acgttcgtta cgggctgcgg ctgctgcgga agaggccgac gtttacggcc
     1201 gtggtggtgt gtacgctcgc tttgggcatc ggcgcgaaca cggcgatctt ctcggtcgtc
     1261 tacggcgtgc tgttgaggcc gataccgtat gtggacggcg agcggctcat cgtgattgag
     1321 acgggtaaga agcaggccgg ggccgagcag ttgggcgggg tcgcgccggc cgacttctgg
     1381 gacttgcagg agcagagccg cagcttcgag cacgtggcgg ccatctcggg cggcgggttt
     1441 agcctgaccg gggtggagaa cccggagatg atcccttcgg cgcgcgtctc gtcgggcttc
     1501 ttcggcgtgc tcggcgcgcg acccgctttg ggccgcgcct tcacgaagga ggacgagctg
     1561 ctggcgtcgc cggacgcggt catcctgagt cacgggctgt ggcagcgacg cttcggcggc
     1621 gacccttcgg tcgtcggccg cgcgttcggc gacacgggcg cgaccatcgt cggtgtgatg
     1681 ccgccggact tcaagtttcc tgtcacggcc gagtgctgga cgccgttgga gcgcgactcc
     1741 ggcgagatga ggaaccgggc gaacagctac ttcaacgtcc tcggcctact cgcgcccggc
     1801 caaacgacgg atggcgcacg ggccgagatc gagactgttt tcgcccgcct ctccgccgag
     1861 tatccgaaga cgaacgccga gcgggcggcg acgctcacgc ctttccgcga ggtgctcgtg
     1921 cgcgacgtga ggccatcgct ccttgtgctc ctcggcgcgg tgggcttcgt gctgctggtg
     1981 gcgtgcgcca acgtcgcgaa tttgatgttg gcgcgcgccg ccgcgcggcg cagggaatgg
     2041 gccgtgcgct cggcgctcgg cgcgtcgcgc tggcggctcg tcaggcagat gctcaccgag
     2101 agcgtgatgc tcgcgctcat ggggagcgcg gcggggctgc tgctggcggt gtgggggcgc
     2161 gacgtgctga cggcgatgct gccggaagac ttcgccaacc ttcagctcgc cgaccagtcg
     2221 cgcatcgact cgaccgtgat gctcttcaca ttgggcgtgg ccctcgtcac cggcgtcgtc
     2281 ttcggcatct tcccggcgtg gcaggcgacg cgtctgaagt cggccgaatg gctgaaggag
     2341 tcggggcgcg gccacgtcgg gacttcgggg cagcgcactc gcagcgcgct cgtcgtcatg
     2401 gaagtggcgc tggcgctcgt cctcttagtc ggggcgggcc tgctgcttca gagcttcgcg
     2461 cggctgcggc aggtcgatct cggcttcaac ccgcacggcc tgtcgtcggt cggcgtctcg
     2521 acctcgtttg cgaaacagcc caccgacggg gcgcgcgcgc agttcgtcgc tcaggtgctc
     2581 gaacgcgtct cgcagacgcc gggtgtggag tcggccgtcg tcacgagcgg gaaccctttc
     2641 ccgtatctca acttcaactt caccgtcgag ggccgcccca cggacgccga ggcgccggcc
     2701 gtctacgact cggtcagccc cgactacttc cgcgcgctcg gcgcacgcct cgtgcggggc
     2761 cgcgagttca atgcgttcga ccgcgccgac actacccgcg tcgccctcat caacgagacg
     2821 ctcgcgcgcc ggcacttcgc cggcgaagac cctctgggcc ggcgcgtctc gatcaactat
     2881 ctcgggcagc cgcagctccg agagatcgtc ggcgtcacga gcgacatcaa tcagggcgag
     2941 ccgggcaagg tgaagccgca gatttacgtg ccctacgacc agcagccgtg gctgagcgcg
     3001 tcgcttttgg tgcgcgccgg cggcggcgac gcgggggcga cgaagcgggc ggtgcaggaa
     3061 gccatctggt cggtggacaa gacgcagccg gtcgccaagt tcgagtcggt cgagcagaag
     3121 ctcggcggcg cgctggccga gccgcgtctc tacacgacac tgctcggcgc gttcgccgcg
     3181 ctcgccctgc tcctcgccac catcggcatc tacggcgtga tgtcgtacac ggtggcgcaa
     3241 cgcacgcatg agatcggcgt ccgcatagcg ctcggcgcgc agccgcgcga catcctcagg
     3301 atggtcgtcg gccacggcct gacgctgacg ctctgcggcg tcggcgcggg gttgctcgcg
     3361 gccttcgcgc tgacgcgctt cctgcaaggg ctgctctacg gcgtcagcgc cggcgacccc
     3421 gtgacgttcg ccctcgtgcc cctgctgctc tcgggcgtgg cgctcgcggc ctgctacctg
     3481 ccggcgcgcc gggcgatgcg cgtcgatccg ctggcggcgc tgagggagga gtaaagctgt
     3541 tagcggttag ctattagctt ttagtcagaa cgggggaacg aaggcatttg aaacattgag
     3601 ctgcgagatg gccgagggcg gtcggagttt ggctaacggc taatagctaa ccgctaacag
     3661 ctaagaagag gttgactgat gaacacgatc tggcaagaca tgcgttacgg cgcgcggatg
     3721 ctgtggaagt cgccggtgtt tacggcggtg gccgtgctgg cgctggctct cggcatcggg
     3781 gcgaacacgg ccatcttctc ggtggtcaac gcgctgcttt tgcggccgct gccgctcgcg
     3841 gagtctgagc gtctggtcgt gctctggtcg cagtcgaaga agacgggggc gctcacatcg
     3901 tcttcctatc ctgacttcgc ggacttccgc gagcagaacc gggtcttcga gggcgtggcg
     3961 acgctgcgcc cgcgcggggt gacgctcacc ggcgcgggcg agccggaacg actcgaaggg
     4021 gcgcgcgtct cggccgactt cttcccgctg ctccgcgtgc agccgctcca cggccgcgcc
     4081 tttctgcccg aggaggatcg accgggcggc gagcgcgtcg tcctgctcgg ccacggactg
     4141 tggcagcgtc gcttcggcgg cgacccttcc atcgtcggcc gcagccttct gctcgatgag
     4201 cagccgcaca cagtggtcgg cgttctcccg caggacttcc gtttccccta cacacttgaa
     4261 aacgcggaag tctattcgac catcgcctac gagggcggca acttgcagga gcgcggcgcg
     4321 cgcacctctt acatgctcgc gcgcctgcgc gagggcgtga cgattgatga ggcgcgcgcc
     4381 gacgccgcgc gcatcgccgc cgggttggag gagcagtacc cggataggga caccgggatg
     4441 ggcgcgaccg tggtcggcct tcaggagcaa cttgtcggca aggtggagcg cgcgctgtgg
     4501 gtgctgctcg gggcggtcgg cctcgtgctc ctcatcgcgt gctcgaacgt ggcgaacctt
     4561 ttgctcgtgc gcgcgacgac gcgccatcga gagatcgcca tccgcacggc gctgggcgcg
     4621 tcgcgctggc gcgtcgtccg gcaactgctg acggagagtt tgatgatcgc cgcactcgcc
     4681 ggcgtgtgcg ggctgctgct cgccgtgtgg ggcgtcgatt tgctcgtcgc cctcagcccc
     4741 gacgatttgc cgcggctcga cgcggtgggc ttagacgctc gcgtgctcgc gttcaccttc
     4801 ggcgtctcgg ccctgacggg cgtgctcttc ggattagtcc ccgcgctcaa ggcgtcgaag
     4861 accgagctga gcgaatcgct caaagagggc gggcgcagca cgaacatgag accggggcgg
     4921 caacgcctgc gcgggctgct cgtcacgggc gaggtggcgc tcgcgctgat gctcttggtc
     4981 ggtgcgggcc ttttgctcaa gagcttcggg cggctgcaag cggtcgatcc gggcttcgac
     5041 ccgaagggcg tgctgacgat gcggatcaac ctgccgcgcg cgcgttacac cgacggcgag
     5101 agccgcgcgc gctttgtcgg cgaggtggtc gagcgggtga gggaattgcc cggcgtcgcc
     5161 gcggccgcct tcgtcacgcc cgttccgttt agctccagta acgtcagcag cgacttcgcc
     5221 gtcgagggac ggcccgacgc gccggtgggg caggagccgt cggcgagcta tcgcggcatc
     5281 acgcccgact acttccgcgc catgaacatt cccctgacgc gcgggcgtca gttcacggag
     5341 caagaccgaa ggggcggccc ggccggcgtc gccatcatca acgaggcggt ggcgcgtcgc
     5401 tacttcgagg gcgaagaccc actcggcaag attatcaaga gcgtgggcgt cagcgtggac
     5461 gacgacgagc cggagcgttg ggagatcgtc ggcgtggtcg gcaacgtccg ccacgcaggg
     5521 ttagacgttg agccgtttgc ggaaatctat ttcccgcacc ggcagcagtc gtggaactgg
     5581 ggccacttcg tggtgcgcgc cgacggcgac ctcgcggcgc tcgccccggc cgtgcgctct
     5641 caggtacacg ccatcgaccg cgatcaggcc atctccgaag tcaggccgct caccgagatg
     5701 atcgccggca ccgtcgccaa gccgcgcttc tacgcgctgc tcctcgcggc cttcgccgtc
     5761 gtcggtctgc tgctcgccgt cgtcggcatc tacggcgtcc tcagctacac ggtcacggag
     5821 cgcacgcacg agatcggcgt ccgcatggcg ctcggcgcgc agcgcggcga catcctcaag
     5881 ctggtcgtca cgggcgggat gaagtatgcg ctcgccgggg tcgtcgtcgg actgttgggc
     5941 gcgcgcgccg tcacgcacgt tctctcgaat ctgctcttcg aggtcagcgc cacggacgcg
     6001 cctactttcg ccggcatcgc gctcctgctc gccgccgtcg ccctgctcgc ctgctacctg
     6061 ccggcgcgcc gcgcggcgaa ggtcgatccg atggtggcac ttagatacga gtgataagtg
     6121 atgagtgata agtgataagt gataagagta aaaccggctt cctctttctt atcactcatc
     6181 acttatcact tatcacctcc gaaggagact ctgatgggaa cactgctgca agacacgcgt
     6241 tacgggttcc gcatgttgcg gaagcggccc ggttttacgg ccattgcgat tctcgcgctc
     6301 gctttgggca tcggggcgaa cacggccatc ttctcggtgg tcaacgcggt gctgctccgg
     6361 ccgctgccgt ttgcgggggc ggagcggctc gccaccgtcg aggtgcgtag cgagcggacg
     6421 ggcgccgtcg gcgccgatca ttcgtacccg aatctggttg acctgcgcga acaggccggc
     6481 acgctcgaac acctcgccgc ctacagcatg acgaccgcct ttctcgcggc cggcgacgag
     6541 ccggagcgcg tgcgcggcct gctcacgacg gccgacctct tccccgcgct cggcgtgcaa
     6601 ccgttgcacg ggcgcgtgtt cacgcgcgag gaagaccaga agggcgcgac gcgcgtcgtc
     6661 gtcctcagtc acgggctgtg gcagcgacgc ttcggcggcg accccgcaat agtcggcaag
     6721 gaaattcccc tcggcggcat caggagcacg cccacgctcg tcctcggcgt gatgcctgag
     6781 ggttttaagt ttcccgtcgg ggtcgccaac gtcgattact ggatgccgct cgtccccatc
     6841 ctttccgatt cggatttgag tcgcgcctcg gtctggctga cgggcctcgc caagctcaaa
     6901 gagggcgcgt cggaagaaca ggcgcgctcc gagctgcaaa cgatttcccg gcggctcgaa
     6961 gagcaataca aggactcgaa cgccgacgcg accttcatgc ttcgctcgta ccacgaacag
     7021 cttgtgggcg acatgaagac ggcgctcctc gtcctgctcg gcgcggtcgg gtgcgtgttg
     7081 ctcatcgcgt gcgccaacgt cgccaacctg atgctggcgc gcgcggccgc gagggggcgg
     7141 gagatttcca ttcgcaccgc tctgggcgcg agccgcgcgc agattgtgcg ccagcttctg
     7201 acggagagtg tgctgctctc catcctcggc ggggcgctcg gactgttgat cgcgtggtgg
     7261 ggcgtggacg tgctcgtcgc ggccgtgccc tcggatttgc cgcgcgccgc cgaggtcgcg
     7321 ctcgacgcgc gcgtgctcct gttcaccgcc gcgctctcga ttctgacggg cctcatcttc
     7381 ggcctcgccc cggcgcttca ggcgtcgcgc gccgacgttt ccgagtcgct caaggagggc
     7441 gggcgcggca gcgtcgaagg cgggcgcagc ccgttgcgta gcgtgctcgt cgtcgccgag
     7501 gtggcggtgt cgctcgtcct gctcgtcggc gcggggttgc tcgctcagag cttcgcgcgg
     7561 ctgatggacg ttagccccgg cttcgacgcc gagcgtgtcc tcgccttcga tgtcgtcttt
     7621 cgcggcgaga actacacgca gcgcgccgcc accgtccccg cgctcgaaga acttctgacg
     7681 caggcggcgg ccgtgccggg cgtcgaggcg gtcggcgtcg tcaacccgct gccgctcggc
     7741 ggcaacttcg aggcaaacag cttccagatc gagggcagag cgccggccga gccggggcgc
     7801 gagccgtcgg ccgaccggcg cgtcatcacc tccgactact tccgcgcaat gggcatccat
     7861 acggaaggcg ggcgcgtgtt cgactcgcgc gacgcggccg cggccacgcc cgtcgccatc
     7921 atcaaccaga ccttcgcgcg ccagcacttc cccggcgaga accccatcgg ccgccgtctg
     7981 cttttcacca acacgcagaa cgtcacgacg gcgcgcgaga tcgtcggtct ggtggcggac
     8041 gtgcgccacg ccggtctgga cgccgagtcg gggccggagt tctacgtgcc cttcgcgcag
     8101 gcgccggccg cgcgcgtgac ggtggtggcg cgcaccacgt cggacgaccc ggcacagctt
     8161 gcgcccgcgc tgcgcggcgc cgtccgtcag gtggacaaga acttccccgt ctacaacgtc
     8221 cgcacgatgg agtcgtacct cgcggagtcg gtcgcccgcc gtcgcttcaa catgacgctg
     8281 ctcggcggct tcgcgctggt ggcgctgttg ctggcgggcc tcggcatcta cggcgtgatg
     8341 agctacacgg tggcgcaacg cacgcacgag atcggcgtgc ggatggcttt gggcgctcaa
     8401 gcgcgcgacg tgctgcgact cgtcatccgg caggggatgg cgcccgcgct cgcgggcgtc
     8461 ggcgtgggac tcgtgtgcgc cctcgcgctc acgcgcgtca tgtcgagcct gctcttcggc
     8521 gtcaccgcca ccgaccccct gaccttcgcc ggcgtcgcgc tcctgctctc ggtcgtggcg
     8581 ctcgtctcct gttacctgcc cgcgcgccgg gcgacgaagg tcgatccgat ggtggcactg
     8641 cgctacgagt gataagtgat gagtgataag tgataagagt aaaaccgtct tcctctttct
     8701 catcactcat cacttatcac tcatcacttc tgagaggtgt tccgatggag acactgctgc
     8761 aagacgttcg ttacgggctg cggacgctgt tgaagcgacc gggcttcacg gtcgtggcgg
     8821 tgatgacgct cgctctgggc atcggggcga acgccgccat cttctcggtc gtcaacggcg
     8881 tgttgctgcg tccgctgcct tacagggacg ccgaacggat catgaccgtg tggcaacgca
     8941 acgcgcagac gggcgcggag cgcgagccgg tctcgccgcc gaacttcctc gacttccgtg
     9001 agcgcaatca gtcgttcgag cagttggccg cgctccgtcc ctacggcctc gactacacgg
     9061 gcgcgggtga gccggagacc tttcagacgt ggcgcgtcac cgaaggcttc ttcgaggcgg
     9121 cgggagccgt cgcgctccac gggcgcacgt ttctccccga agagtatcgc gacggagtcg
     9181 atcaggtggt cgtcatcggc cacggtctgt ggcagcgacg cttcggcggc gacccaggcg
     9241 tagtcggaca acagctcgtg ctcgacggga agccgcgcac ggtcgtcggc gtcatgccgc
     9301 ccgagttcca ctacctcgac aagcgcgaga tgatcgcgcc ctaccatatc gacgcgcacg
     9361 agctgcaacg acgcgcggcg acttacctga acgtcgtcgg gcggctcaag cccggcgtca
     9421 cgctggagca agcgcgctcc gacatgagca acgtggccgc gcgtctcgcc gaggaatacc
     9481 cgcaggccaa cggcgggatg ggtgcggcgg tggcgccgct cgccgagcag atgttggggc
     9541 gcgtgcggcc ggcgctgtgg gttctgttcg gggcggtggg cttcgtgctg ttgatcgcgt
     9601 gcgccaacgt cgccaacctg atgatcgcgc gctcggcgtc gcgggagagg gagttcgcca
     9661 tccgcgcggc gctcggggcg gggcgcggac ggctcatgcg gcaactgctg acggagagtc
     9721 tggtgctcgc cggcctcgcc tgcgccggcg gcctcctgct cgccagttgg ggcatcgacg
     9781 ccatcatcgc cctcagcccg gcggagctgc cgcgcatcga tcaggtgaga ctcgacggcc
     9841 gcgtgcttct cttcgcgctc ggcgtctcgg cggcgacggc cctcttcttc ggactcctgc
     9901 cggcgctgcg ctcatcccaa cccgaccttc aggagtcgct caaggaggcg agccggagcg
     9961 cgacggccgg cgcggcgcgt catcgcctgc gcggcttcct cgtcgtcacg gagttcgcgc
    10021 tggcgctcgt gctcttagtc ggcgcggggc tgctcgtgcg cagcttcatg cgtctgctcg
    10081 aagtcgatcc cggcttctcg gccgagcgcg cgctcacact tcaggttcac gtctacgacc
    10141 agtatccgaa gccggaacaa caggcggtct tcttcgagca ggcgctcgaa cgacttcgcg
    10201 ccctccccgg cgtcgaggcg gcgggcgcgg cgtcggcccc gcccttcatc ggcgagggcg
    10261 ccatcgagat cgacagcccc atccagatcg agggacagcc cgccccgccg gcgggccagg
    10321 agccgaccgc ctttcacaca atcgtgacgg ccgactactt cggcgcgctc ggcgtcccgc
    10381 tgcgcggcgg acgcatcttt aatcaattcg accgcgcgga cggcgcgccc gtcgtcatcg
    10441 tcaacgagac gctggcgcgg cgctactggc cgggcgagtc ggccgtcggc aagaagctca
    10501 tcgtcctccg gctgagcaag ccggtcgcgc gcgaggtcgt cggcgtcgtc ggcgacgtgc
    10561 gccacaccgg actcgacagc gagccgcgcc ccgagctgtt tttgcacagt ctgcaaaacc
    10621 cgttcgggtc gatgaccttc gtcgtgcgga cgacgcctga gccttcgacg ctgctacagg
    10681 ccgtcaaggg cgaggtgtgg gcggtgaaca agaacctgcc cttctacagc acggcgacaa
    10741 tggagagtct ggtcgcggag acgctcaagg agcggcgctt cagcctgttg ctgctcggcg
    10801 ctttcgcctc tctcgcgctg gtgctcgccg gggtcggcat ctacggcctc atcagctact
    10861 cgacggcgca acgcacccac gagatcggcg tgcgcgtggc tctgggcgcg cgcgggcgcg
    10921 acatcatgcg aatggtcgtc ggagaggggt tgctgctgag cggcgcgggc gtcgcgttgg
    10981 gtctgctggg ggcgctcgtg ctcacgcgct tcctcggcgg gctgctctac ggcgtggccc
    11041 cgaccgaccc gctgaccttc gccggcatcg cgctcttgct cgtctgtgtc gccctcgcgg
    11101 cctgctacct cccggcccgt cgcgcggcgc gggtcgatcc gatggtggcg ttgagatacg
    11161 aataacaaga ggttagaggt tagaggttag aggtcagtta aaactgattt cactaacctc
    11221 tgacctctga cctctgacct ctctggagat tgttatggga acactgctgc aagacattcg
    11281 ctatggactg cgcgggcttt ggcggcaacc ggcgttcacg ttggtggccg tgtgttcgct
    11341 cgctttgggc attggcgcga acacggcgat cttcagtctg gtcaatgccg tgttgctcaa
    11401 gccgttgccg ttcgccgact cggagcgcct cgtcatggtg tgggaagacg cctccgtcat
    11461 cggcgccccg cgcgacagcg tggccccggc caacttcgtg gactggaaga cgcagaatca
    11521 ggtcttcgac gagatggcgg tgctcagttg gcagagcttc aacatcaccg gcgacggcga
    11581 gccggagcgg gttacggctt acgccgtgac ggcgaacgtc ttcccgctgc tcggcacgga
    11641 gccggcgctc ggacgccatt tcctgcccga agaaaatgag ccgggtacgg ctaaagtggc
    11701 gatgctcagc cacggcctgt ggcagcggcg ttacggcggc gacccgtcga ttgtcggccg
    11761 cgacatcatg ctcaacggcg agaagcgcac ggtggtgggc gtgatgccgc agggctttca
    11821 gtttctccag agcttcatcg gactctgggt gccgacggcg cttacacagg aagaactcgc
    11881 caaccgcggc gcacactacc tgaccgtgct cgcgcgcctc aagcccggcg tgacggccga
    11941 acaagcgcag gccgacatcg gcgccatcac cgaccgcatc gcgcaagccg acccgaacga
    12001 ggccggaaac ctgaaggcgg ccgtcgtgcc gctacgcgaa caactctcag gcggcgtccg
    12061 caactcgcta tttatcctgc tcgccgcggt cggcttcgtg ctcctcatcg cctgcgccaa
    12121 catcgccaac ctcctgctcg cccgcgccac ggccaggcag cgcgagatcg cggtgcggac
    12181 ggcgctcggc gcttcgcgct ggcgcgtcgt gcggcagttg ttgacggaga gcgtgctgct
    12241 ctccgtgatg ggcggcgggg ccggtctgct tttggccgcg tggggttttg agtttctgaa
    12301 aaaactcgtc cccgacgcga tggcgctctc ggccacactg aaactcgatc tgccggtgct
    12361 cggctacgcg ctggcggtat ccgtgctcac cggcctcgtc ttcggcctcg cccccgcctt
    12421 gcaggcgtcg cggatcgatc tgggcgaagc cttgaagcag agcggcgggc gcacgggcct
    12481 gacggcgggg ggagggcgtc tgcgcggcgc gctcgtcgtc gtggaagtgg cgctggcgct
    12541 cgtgctcctg gttggcgcgg ggctgttgat tcagaccatc tctcggcttc agcaacagta
    12601 cacggtcttc cagcccgaac agttgttgac gctgcggacg gtgctgccag aaggcaagta
    12661 caaggagctg tcgcggcgcg tggacttcta cgatcaggtg ctcgcgcgtg tggagacgct
    12721 gcccggagtc gtctcggccg gctacaccac ctcggtgccg ttgcagtgga agggcggggc
    12781 caacggcttc acgatggagg ggccgccgcc cgagggggtg ctcgccacga acgccatcca
    12841 ccggcaggtg agcgccggct acttcaagac gatgggcgtg ggtcttcgcg agggccgcta
    12901 ccttgaggaa cgcgacgacg cgaaggggca gcaggtgatg gtcgtcaacg agacgatggc
    12961 gcggcagtat tggccgggtg agacggccat cggcaagcgg atcaagttcg gccccgccga
    13021 aggcactgac ccgtggcgca ccatcgtcgg ggtggtggcc gacgtgcggc agatgggcct
    13081 cgacgcgccc gtcaaggcgg agatgtatct gccgtaccgg cagattgcgt cgcacgcctt
    13141 ttacaagccg cgcgatctgg tcgtccgcac cagtgttgac ccgatgagtc tggtgagcgc
    13201 ggtgcgcggc gagattcacg cggttgaccc tgaacagccg ctctcgaaca tcgcgacgat
    13261 ggagaagctg ctcgccgacg agaccggcgc gcgcggcctc ggcatgatgc tgctctcgac
    13321 cttcgcgggc ctcgcgctcc tgctggccgc gctcggcatc tacggcgtgc tggcctactt
    13381 cgtcacgcag cacgtgccgg agatcggcgt ccgtctcgcg ctcggcgcac gcccgcgcga
    13441 catcctcgcg ctggtgctca agaaggggat gggcctcgcg ctcggcggcg tggccgcggg
    13501 gcttctggcg gccttcgcgc tcacacgcct gatggagaaa ctactcttcg gcgtgacggc
    13561 gaccgacccg gccaccttcg ccggcgtcgc agccctactc gtcgccgtcg ccttcgccgc
    13621 ctgctacatc cccgcgcggc gcgcgacgaa ggtcgatccg atggtggcac ttcgctacga
    13681 ataagaggtc agaggtcaga ggttggaggt cagtaaaaac tgtcttcact aacctctgac
    13741 ctctaacctc tgacctcttt ggagattgtt atgggaacac tctggcaaga cttacgcttc
    13801 ggcatccgca tcctgctgaa gaagccgggc ttcacggccg tggcggtgtt gacgctggcg
    13861 ttagggatag gggcgaacac ggcgatcttc agtgtcgtca accgggtctt gctcaggccg
    13921 ctgcctttca cgcagcccga acaactcgtc cgcgtgaccg ccgatattca gaagacgaac
    13981 tcgactgatg tcggcctttc gggtttggag ctgttcgatt accgcgaccg cgcggggatg
    14041 ttcgagcaaa tctccggcgt gtatcccatc aacgcgaaca tcaccgagat cgaccagccc
    14101 gagcgggtcg aggcgttgct cgtggatgtc aattacttct cactgctcgg cgtcgaggcg
    14161 cagctcgggc gcaccttcca gcaggaagac taccagccgg gcatcgccga agtcgccgtc
    14221 atcagcgacg ggctgtggcg gcggcgctac ggcgcgggca cggacgtgat cggcaagaag
    14281 ttccgtctcg acgaagacct ctacgtgatt gccggcgtcg cgcccgcgag cttccgtcac
    14341 cccggccgca cgcttcagac cgacgtggag gtgtgggtgc cggccgggtg ggtcggcccg
    14401 ccgttcaaca acccgccgcg cggcgcttat tttttggcgg gggcgattgg ccggctcaag
    14461 cccggcgtca cgctccccgc ggcgcaggcg cgcctggccg aactcgccgg cgagctgcgc
    14521 cgggagtacc cgaacgacta cccggagggc gggggctgga cgccgcgcct cgtcggtctg
    14581 caagacgatc tggtcggcaa cgtgcggccg gccctgttgc tgctgctcgg cgcggtcggc
    14641 ctcgtgctgt tgatcgcgtg cgctaacgtc gccaacctgc tcctgctgcg cgcgtcggcc
    14701 cgccagcggg agatcgccat tcgtcgcgcg ctcggcgcgg ggcgcatcag gctcatccgc
    14761 cagttgatga cggagagcgt gctgctcgcg gtggcgggcg gcgcgttggg tttgctcatc
    14821 gcggtctggg gcgtcgagac actggtgaag ttcagcccgg ccaacatctc gcggctcggc
    14881 gagatcggcg tggatgcgtc ggtgctcgtg ttcacgctgg cggtgtcgct cgtcacgggc
    14941 gtcgtcttcg gactcgtccc cgcgattcag gcttcccgcc cgaacttgca ggagagtttg
    15001 aaagacgcgg cgcgcggggc gaccgccggc gcggcccgca accgcatgcg gagcgtgctc
    15061 gtcatttcgg agttcgccct ggccctgatg cttctcatct cggccgcgct gctgatccgc
    15121 agcttctgga tgttgcagag cgtgaatccg gggtttcggg cggagaacgt cctgacggcg
    15181 cgtctctggc tgccggcgcc gaacgatagg aagaccggcc cctacttcac acaccagagt
    15241 cgcgtccaga tgtacaaaga ggcgctccag agactcgccg cgctgccggg cgtcgaacgg
    15301 gtcggcggcg tcagctccct gccgctcgac ggcgggcgcc ccacggcccc gttcaccgtc
    15361 gagggccgcc cccccgagtc ggccaacgtg aacacggtgg ggtttcacta cgccagcccc
    15421 ggctacttcg acgccatggg aattcccctc ttgaagggca ggggcttcgc cgaacaggaa
    15481 gacgagaagg cgcccggcgt cgtcctgatt agcaggacga tggccgagcg tttcttcccc
    15541 ggcgaagaac ccctgggcaa acgcgtcagg ctcggtggtc acaactcgaa agcgccgtgg
    15601 ctcacggtcg tcggcatcgt gggcgacgtg aagagcgagc ggctcgacgc cgagaaccag
    15661 ccacagatgt acacctcgct gcttcagaac tcgaacctct tcatggcctt cgtgattcgc
    15721 acggcgaata acccggccgc gctctccgag gccgtgcgca gcgaaatccg ggcggtcgat
    15781 cccgatctgc ccgtccacgg cgtccgcacg atggaggagg tgatgtcggc gacggtggcg
    15841 cagcagcgct tcgccatgac gctgctcggc gtgttcgccg tcatcgccct gcttctggcg
    15901 gcggtgggca tctacggcgt gatgtcttac gccgtcgccg agcgcacgca cgagatcggc
    15961 atccgcatgg cgctcggcgc gcaggcggga gacatcgtgc gcatggtcgt caggcagggg
    16021 atgattctcg cggtcgtcgg cgtcgtcgcc ggggccgcgg ccgcgctgct gctaacgcgc
    16081 gtcatgtcga gcatgctcta cggggtgagc gcgagcgacc ccgtaacgtt cgccgccatc
    16141 tcactcgccc tcgccgccgt ggccttcctc gcctgttacc tgccggcgcg gcgcgcggcg
    16201 aaggtcgatc cgatggtggc actgagatac gagtgataag tgatcagtga taagtgatca
    16261 gaaagagcaa gacggtttca ctcttatcac tcatcactta tcacttatca ccctccgaag
    16321 gaggatgtga tgagaacact cctgcaagac ctgcgctacg gattgcgcgt gctgcggaag
    16381 agtccgggct tcacgctggt agcagtggtg tcgctcgctt tgggcatcgg ggcgaacacg
    16441 acgatcttca gcatggcgaa cgcgctgctg ctgcgcccgc tgcccgtcga tgagccggag
    16501 cggctcgtca gcctccactc ggtcagtccc gacggcagct ccttccacac gttctcgtac
    16561 cccgactatc aggactaccg cgaccagtcg gacgtgttcg agggactact cgtctacacc
    16621 atcgacacgt acagcctcaa cgccgacgag cgcaccgagc gcgtcttcgg gatgctcgcc
    16681 tctggcaact tcttcgacgt gatgggagtg cggcccgcgc tcgggcgctt cttcacgcct
    16741 gaggaggaca aggccccgcg cggcgaggcg gtggccgtct taagcaacgg cttctggcag
    16801 cggcgcttcg gctccgaccc ctcaatcgtc ggcagggaac tgctcttcgg cggccagccc
    16861 tacacggtgg tcggcgtcgc cccgcgcggg ttcacgggca cgcgcgtcgg cttcgcgccc
    16921 gacgtttatt tgccgatgtg gtcgcacggc cggagcgcgc gcggctccga ggggctgaac
    16981 gaccggagcg gcgggagtct ggagatcgtg gggcgtctga aacgcggtgt gagtgtcgag
    17041 caggcgcagg cgcaggccgg cggcgtggcg gcccggctcg ccgaagcgta cccggattcg
    17101 caccggggca aaggtgttga ggtcaggcgg ctcggcacgg gcctcggtca gttcgaggct
    17161 ccggtgatgg gcttcatggg cgtgctgtcg gcggtcgtcg ggctggtgct gctgatagcg
    17221 tgcgccaatg tggccggcat ggcgctggcg cgctcggcga cgcgccggaa agaggtcgct
    17281 atccgtctcg cggtgggcgc gcggcggagt cgcatcgtgc gccagctcgt cacggagagt
    17341 gtgttgctgt ttctcatcgg cggcgcggcc ggcgtcctgc tcgccgcgtg gctggccgac
    17401 ttgctgatgg ccttcaagcc gcccacgccg ttccccgtcg agcttaactt cggcctcgac
    17461 tggagggtgc tcgtctacgc gctcggcctg tcgctggtga cgggcatcgt cttcggactc
    17521 gccccggcgc ttcaggcttc gcggcccgac gtgattccgt cgctcaagga tgagtcgggc
    17581 ggcggcttcc ggcgctctcg tctgttgaac gtcttcgtcg ccgggcagat cgccgtctcg
    17641 ctcgtcctct tagtctgcac cggcctcttc ctgcgctcac tccagaacgc gcgctcgatg
    17701 gaccccggct tcaaccccga cggcgtccag ctcgtcggac tcgacctccg catacagggc
    17761 tacgacgagc cgcgcggccg acgcttctac gctcagcttt tggagcgact gaagtcggcg
    17821 cccggcatct cgtccgtgac gctggcgcga atgatcccgc tcgacggcgg gaacatggaa
    17881 tacggcatca acgtcgaagg ccgcgagccg cccgacggcc gccgcagctt ctcgaacgac
    17941 acgaacgtcg tcggctccga ctacttcaag acgctcggca tcccgctcgt ccggggccgc
    18001 gacttcgggc cgcaggacgt taaggaagcg ccgcgcgtcg ccgtcgtcaa cgaggcgatg
    18061 gcccggcact actggccgaa cgaaacggcc gtgggtcgtc gcttctacat cggctcgttc
    18121 gccgatggcg tccccgtcga gatagtcggc gtggcgaagg acggcaagta ccgcacgctc
    18181 ggcgaagaag cgcgccccta cctctacctc gcggccgagc aggactacag ctcttacctg
    18241 acgctccacg tccggccgcg ctcggcggag acggcggccg atgctctggc cgccgtgcgt
    18301 cgtgagacgg ccgcgctcga tcccaacctg ccactgctcg acgtgatgcc gatgaccgag
    18361 gcgattggca cgtcgttgtt gccgattcgc atggcggcga cggtggcggg cgtgctcggc
    18421 gtggtggggc tggtgctcgc cggggtcggc atcttcggcg tcgtcagctt ctcggtcgtg
    18481 cagcgcacgc gcgagatcgg cgtccgcatg gctctgggcg cgcagacgcg cgacgtgttg
    18541 cgactcgtca tctggcaggg catgaagctc gcactcgccg gcctcgccgc ggggctgctc
    18601 ctcgccatcg cgctctcgcg cgtgctctcg ggcctgctct acggcgtcag cccgacggac
    18661 gtgtccgtct tcgcaggcgt ctcgctcctg ctggcgacgg ccgccctcgc cgccagctac
    18721 atccccgcgc ggcgcgcggc gaaggtcgat ccgatggtgg ctctcagata cgagtgataa
    18781 gtgatcagtg atgagtgata agagaaagac agattctttc tcatcactta tcactgatca
    18841 ctcatcactt ttcatagagg tgttgtaatg gacaaactgc tgcaagactt gcgatacgga
    18901 gtgcggacgc tggcgaagcg gccgggtttt acggccgtgg cggtgctgac gctcgcttta
    18961 gggatagggg ccaacacggc catcttctcg gtggtcaata cggtgctctt aaatccgctg
    19021 ccgtatgagg gcgccgaccg gctggtgatg ttgttcgagc agacgccggc gcaggagcgc
    19081 aacttcatct cgcgtccgaa ccttcaggac tacgccgagc agagccgcac gttcgaggag
    19141 ttctcggcga tggtcggcca gagcgtcaac ctgacgggca cggagcagcc cgaccggctc
    19201 atcggcaact tcgtcacgtc gagcttcttc cgcgtcctgc gcgcgcgacc cctccacggc
    19261 cggctcttcg aggccaacga ggatgctgag ggcgccgagc gcgtcgccat cctcagtcac
    19321 cccacttggc agacgcgctt cggcggcgac ccctcgatag tcggccgcgc gctcactctg
    19381 aacggcgagc cgcacaccgt cgtcggcatc ctgcccgaag gcttccgctc gccgggcagc
    19441 gactacgacg tttacatgcc ggcgcagttc tggcccaact acaaggtggc gcgcgac
//
LOCUS       DASVES010000024        19246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1140670, whole genome shotgun sequence.
ACCESSION   DASVES010000024 DASVES010000000
VERSION     DASVES010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19246
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140670"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..1725)
                     /locus_tag="VF240_00930"
     CDS             complement(442..1725)
                     /locus_tag="VF240_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7173833.1"
                     /translation="MSDRLRKVIHAFSAVADLGVEIADTSDFDEMVTAALQVVLGALG
                     VRRGAVGEYDADAGVMRFVAARGHAEGAPSEVPFSPMEAEALVGGEGAAVSVEAEEMS
                     ADDKRVSLSERFLSELERSRVKLLVPLLVHGRLEGLILLGEKASGEAYTVEDRDVARA
                     LGRHIGVGLHNHRLLREIERRAEETRRIYEELRGIYQGTVRAFAAAIDYKDKYTQGHS
                     ERVGKYSEIIARELGWSDEEVEGMAIAGYLHDVGKLIVDRDIINAPYKIDAKQSSELN
                     RHPAAGYEILSPIKHPYADIPLTARYHHERVDGRGYPDGLSGDEIPLCAKIVTLADSF
                     DAMTTDRPYKRRREFPEVVEDLRRNTGQQFAPEVVLAFCRALLKEVRSEPGQQQRPMM
                     RLLGKGYLDAAQGEPMLTQLITELESGATAPVGSA"
     gene            complement(1743..2126)
                     /locus_tag="VF240_00935"
     CDS             complement(1743..2126)
                     /locus_tag="VF240_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7173834.1"
                     /translation="MNESARSGSPALAPSRAGDVAVVYAGDYVNKMSGQRLERECRLR
                     LEEGARTVVINFRDTELVNSIGVSILLGIVDIAEARSARLVFTNLNRHNQKLFELLGL
                     TRCVALADSEEAALAEFAAPAPRGH"
     gene            complement(2142..4439)
                     /locus_tag="VF240_00940"
     CDS             complement(2142..4439)
                     /locus_tag="VF240_00940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7173835.1"
                     /translation="MPTRKTNKVTGETQKKRRILGRVAPEDFVGRGAELRLVGDLAAH
                     TSGPRNLLVLAAPMAGVSELLRQSFDTLFQERGGASPVYFSFSRRDQGAGGAALRFLQ
                     SFVVQTVAHRRGDPDLIFSPPTLGGLLDLITPSDYDWVERIVSASERARGEQDEAALI
                     RLCLGAPQQAAAHGARTLVMIDDVHLAAELDAEGGLGAELAQMAAQTDTPFVLAGLRR
                     SFSDVMHLAEEAHRAADTGLIHLERLSDEDARALATVVAARLGVNLSDETRDLMVQQF
                     EGSPQLITALIRTAQGAASDLGTFRDFQRLYVDDLLGGRINRRFNAALEEIAPSIDTR
                     RALVRVLYETAENVEGKSPVEAWRRRLGVEGAELKKVLTGLHVRELAGFDASYVETSR
                     SLVWHDYLRASYRLHVAVEPRALVVANTLVDALKRAPQTMARYYRREAALGLRELLER
                     FNLQRVPASLLHNDRFQRTYRGATPEEVAAGLEAETDLVRLPQVVHAASCASFHPPVQ
                     LVIDEERCAVAHGFDSSPYSEHNEVVWIAAEIDSKLEAGRALAEVWLDRLTQVVHACG
                     FRRARLWLVAPEGFSEDANKLLNVREAFGSSRAQLELLAARLDTGGAGAHVSEAASNE
                     FEMIMPMGDDTELIAAHTVEQIARRLEFDPEAISQIKHALVEACINASEHSLSPDRKI
                     YQRFRLESDKLVVTVSSRGVAPPPDATISGGYALAHTNGREANDSIPGRRGWGLKLIR
                     TLMDEVEFERVDDGTRLRMTKYLRK"
     gene            complement(4417..6639)
                     /locus_tag="VF240_00945"
     CDS             complement(4417..6639)
                     /locus_tag="VF240_00945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7173836.1"
                     /translation="MDKAASAPEHAENPSAATVPTGWAEVQESLAAASGLALLLVEGR
                     QPPALRVSHNNSICQAFQSSPKHAHLCDPFCGRAFERAQEAAGPAHYRCHAGLHCFAA
                     PLDLGAAERLAVIAGRAFLRSSDYRVLAERMRSGDLSDLLSPDLFRNVIFALEQDLAD
                     LAVRLREAAEEFPKGARGSRKSAATGRGVGAPAAGKADAGVGKREAPEAEKSNGVDEA
                     SEARGARRGAAPAPAAMPHANGSAPSSQARGFASNTSLAEACEKAVRKLCESHGVESV
                     ALMLRADEKFTSVYTTGQFRAQPPRVSLKAKEIKLLQAATHGESIAVPAGGRTATRHE
                     DAVELFPLAVGEEMKGALLVGDKHLSEEQRRAVAAFCREIALPLEVLRLREELERRMR
                     TATHLRAFVERINSAPPEEAYQMILRQSAEMLRAERGSLLLYDEAADELSMKAMVGPR
                     TEAAQDVRLRMGEGVAGTVMREGRPLVVSDVALAGWKPAPAERSYKTKSFISYPLSIS
                     GRKVGVLNVTDKAGGGNYDDVDLSLLDLIAPQMALALDRAQWHQKATQFQLLSITDPL
                     TGLLNRRYLEERLSEELERSKRHQFQMSFMMVDIDDFKHYNDLNGHQAGDLALEMTAQ
                     CLKATLRSADVAARYGGEEFCVLLPQTSLSEAVVIGERIRRRIERTQFPHDKAQPAGN
                     VTVSIGISAFGSGVDTPASVIYAADQALYVAKSRGKNCVNAYRSEAEKNSKDADAEDK
                     "
     gene            complement(6906..8048)
                     /locus_tag="VF240_00950"
     CDS             complement(6906..8048)
                     /locus_tag="VF240_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7173837.1"
                     /translation="MSTQGIDQTGAAGMPPTLFPAEGAEPLIEIRELKVHFDLGGGTF
                     LDKLTGGSSVRRVVKAVDGVSIDIFPGETLGLVGESGCGKSTLGRALLRLTEPTGGQV
                     FFRGRDLAHLSQKELRKHRRHLQMIFQDPYASLNPRMTVGQIIGEPVDTFGLAQGRHE
                     RNERVRELMETVGLSPRFVKRYPHEFSGGQRQRIGIARALAVDPDFIVADEPISALDV
                     SIQAQIMNLMERLQQERNLTYLFISHDLRAIRHVSDRVAVMYLGKLVEVAGAKTIYDD
                     PLMPYTRALISAVPVPDPEAEAGRQRIVLQGDVPSPINPPSGCRFHTRCPYAIRECKE
                     VVPPLAEIKPNHFAACIRISPDQPDIERVAPGDAPGLRNPGRIIAQ"
     gene            complement(8048..9148)
                     /locus_tag="VF240_00955"
     CDS             complement(8048..9148)
                     /locus_tag="VF240_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7173838.1"
                     /translation="MSSNNGTLLSVRDLRTYFDTEDGVVKAVDGITFDLERGETLGIV
                     GESGSGKSVTNLSIIRLIPQPPGRIVSGEVIFHGQDILTLSTDEVRKIRGKRISMIFQ
                     DPMTSLNPFMRISKQLMEITRLHLGHTKDQAYEHAIKMLEIVGIPDARTRVDSYPHEF
                     SGGMRQRVMIAMALSCEPELLIADEPTTALDVTIQAQILELIKQLKADTGTSVILITH
                     DLGVVAGMTDHIIVMYAGKVFEQAPTRELFSCPGNPYTKGLLRSVPDPTSEQGKLYQI
                     PGLPPDVAHLPPGCPFAPRCERAEDICRQEFPPFVQLNAEHFSLCHFAEEVYAASCDE
                     RDRRASAAPPGATTDRRDDTTTTTTPPQGDTR"
     gene            complement(9238..10365)
                     /locus_tag="VF240_00960"
     CDS             complement(9238..10365)
                     /locus_tag="VF240_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173839.1"
                     /translation="MNDERTPRVPTEEGRSETPHGASEPEPAFTPLGKPQQPDIRHRK
                     GDQIHTGPELAVGEGDVRTAEPGSAVVAVAGTSLWKDAWRRLLKNRLAVFGMIVVVLI
                     ILASIIGPPIIKATAGFDYDDFPADSSLVKSFPPFTAPDGTFSWLHPMGTDSEGRDIL
                     ARVLQGGRISLAVGFISTIVSLLIGIGYGATAGYLGGKVDDVMMRVVDVLYAIPYMMI
                     VIVLLAFFGSQTPLKQIFLLFAALGAISWLTMARIVRGQVLSLKNQEFVLAARATGVN
                     VPRIIFRHIVPNVLGPVIVYATLTIPQVMLSEAFLSFLGLGVQAPYASWGSLASLGIQ
                     NIAIFPWQLIFPGVTMALTLFSLNFLGDGLRDALDPQMRKF"
     gene            complement(10371..11291)
                     /locus_tag="VF240_00965"
     CDS             complement(10371..11291)
                     /locus_tag="VF240_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7173840.1"
                     /translation="MFPFIIRRLIVTVPMVLIVVTLTWALIRLAPGNFYSGEKKLPAA
                     IEKNIREKYGLDKPWYVQYGRMIGSVARLDFGNSLKYESESVNDILKRTLPVSATLGV
                     LAYLLALVVGITAGTLAALKQNSRLDYASMAVSMLGISVPNFVLGPILVMVFALTLFW
                     LPPARWGGFPSWNLLLPVFTLSAIYMAYIARLTRSGMLEVLRSDYIRTARAKGLKERE
                     VIWRHAMRGGLLPVVSYTGPALAALIAGTVVVERIYAMPGLGNYFINANLNRDEPLII
                     GIVAFISIVILIFNLLVDIAYAFIDPRIRY"
     gene            complement(11483..13591)
                     /locus_tag="VF240_00970"
     CDS             complement(11483..13591)
                     /locus_tag="VF240_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7173841.1"
                     /translation="MFRNRIVTALLASIVLGLAAVAAGCAAQASKSEFFGKIEPPPGQ
                     TLRYISGSEPESLDPHRSSGQPETRIFMALYDGLVEYHPKTMQPIPAIAERWTLDEDA
                     TEIVFHLRRDAKFSNGDPITARDFVYSFRRGLQPELAARSAYLAYEIKYAQGVNEGGS
                     FVRDPATGRFVTAAEAAPPAEKAPAAAAEAAGAAVKPADGTSAAPTKEPGDQAAAQKK
                     EEEPAAAPPATTDPEALHRAELSKKGEDAAPDTEFHKYMHQPTRLVVPSEAKEREKLF
                     KENPKLQGLLAGKEFVPVKPEDLGVEAVDDHTLRLTLMQPAPYFIGLLPHQFFRVIHE
                     GAVERHGVAWTRPENIVVSGAFTLKSHRPYNEVHVVKNQQYWDAATVRLDEIRFYPLE
                     EQTTMLNLYKAGDVDATYNHTVPAAWLKSGVREMKDYMDAPENAIEYYQINTTKAPMN
                     DRRVRKAFAMGVDRKALADYRVVVKPLTAFSPEGIYPGYPQPKGDDFNPERAKQLLAE
                     AGYKDASGKFDPSKFPVSEVEITYNTSESNRAVAEFIQAQWKQHLGMTIPLKNVEWKS
                     FLDMRSKLQYKGFARAGWVGDFMDPYTFLALFSTLGHDNGTGWIDQKYVAKLKAANSE
                     PDQTKRYALLAEAEAYLLDVQPVLPLVTPATSWMKKPYVKGLYPNPGTLHAWKYVYIE
                     HDRAKWDKQMPDMKSESLAD"
     gene            complement(13884..15800)
                     /locus_tag="VF240_00975"
     CDS             complement(13884..15800)
                     /locus_tag="VF240_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7173842.1"
                     /translation="MLKQVIHIFNPIGRGRRARPLVALAFALMLSSQGCFEQERGAAF
                     YGRVAVPAAQEFRWSDGGLPRVFDPAIAASPPDTDVVRAMFEGLTDYEPRTLAPVPAV
                     ATAWEAGEGGRVWTFRLRPEARWSNGDPVTAHDFVRSWKRTLQLGERAPHAGLMRNIE
                     GAPDAGVVPSEVNEPKFFGFDMGQQFRKLGEAAAALPTPVPAPPDFGARASDAQTLVV
                     RLKRPDALFPALVAHPVFRPVHELGAHADLTPAHAGRGKALGAEDEKTLITNGAFRLR
                     GRAEGGVVLERAENYWGASSVQLERVHFVASADAEEALSQYRAGQVDAVTNAAFEPLA
                     LKLLAPHEDFRRQIFGALTYYQFNTTRAPFDDLRVRRALALALDRERLSADTLGGATL
                     PAARFLPGAEASAGVSGEGAKASEAAKTSSDEQRAAPLTFDPVRARELLAEAGYAGGA
                     KFPVIRLLVNRNERQRLLAQAVAGMWREVLGVETEVTLKDWEHYESSLAAGDFDIARR
                     SIVMQTPDEERNLLAMFGAAGLSATTPDETDSTPEAQASQAASDSSKAPPTEEAARES
                     VAAITTEAEALRALPAIPVYFATSFALVKPYVAGFDTNLLDAPSLKYVRIETGWKPGE
                     GTGVSPPAATVSGL"
     gene            complement(16051..16677)
                     /gene="coaE"
                     /locus_tag="VF240_00980"
     CDS             complement(16051..16677)
                     /gene="coaE"
                     /locus_tag="VF240_00980"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7173843.1"
                     /translation="MLRVGLTGSIATGKSFVSGVLGELGCHVIDADRVAREVVEPGTE
                     GLRRIVEAFGQKVLTPEGALDRAVLGGIVFNDEKLRELLNSILHPLIIAAQDERLREF
                     EEVDPEGVAVVDAALMIESGGYKRFEKLIVVHCRPELQRRRLIERDGMSAESAERRIA
                     AQMPQGEKLRYADFRIDTSQGFEDTRRRTEEVYAALRKLAKKKAAGES"
     gene            complement(16790..17773)
                     /locus_tag="VF240_00985"
     CDS             complement(16790..17773)
                     /locus_tag="VF240_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX7173844.1"
                     /translation="MPDRMSSGSREGETGRASVLDGARVAAEIKREVGADVARLAAEH
                     AVVPCLVVVRVGDDPASEVYVRSKVRACEELGIKSEHRQLSAETTGGELLELVEGLNR
                     RDDVDGILVQLPLPKGLDESVVIESIDPAKDVDGFHPTNVGRLALRRPALVPCTPAGV
                     LELLVREGLTIRGAHACVVGRSHIVGHPVAELLLQADATVTICHSRTRDLPAVTREAD
                     ILVAAIGRPGFIRGEHVKEGATVIDVGINRVSDEATIRELFGARAEKRLEGVGRRGYT
                     IVGDVHPAEVEKVAGRLTPVPGGVGPLTVALLMRNTVEAARRRRGMEEGKR"
     gene            17873..18790
                     /locus_tag="VF240_00990"
     CDS             17873..18790
                     /locus_tag="VF240_00990"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7173845.1"
                     /translation="MKRRQSDGSTSGNFFRPAALFALLSVLILGGCVASKGHGEVRRY
                     DLKGKVVSVERGRGEVVVEHETIPGLMDAMTMPFALKDKDALSVVEPGDRLQATLVVA
                     DSGGFWLESPMITKAAPSGESPANESSGGPNPGDVVPDFSLVNQDDKPVRLGRYRGRA
                     LLLTFIYTRCPLADYCPLMSERFAAISRELAGEASLKDKVQLLSVSIDPAHDTPKVLR
                     SYGAAHTQKYGGETFASWEFATGDPEEVKKLAQFFGLEYFAEKDQIIHSLRTAVIDPT
                     GRVHKIYRGNEWQPSEVLNDVRTLFREHH"
     gene            complement(18883..>19246)
                     /locus_tag="VF240_00995"
     CDS             complement(18883..>19246)
                     /locus_tag="VF240_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173846.1"
                     /translation="ESFTAKFVEGEPNVRLELMNTTDVTLKCVEVLTVFLKDEETPGG
                     GPSRAHIKFETVKSIQPKEKVVLSHKTWIDGKPAAARYDELERLKIVEGGVRPYVLDI
                     SWEDAEGKSRYQRIPVGH"
BASE COUNT         3180 a         6159 c         6487 g         3420 t
ORIGIN      
        1 ggcaatgaaa acgctcgaaa aaagaataat ccatgccgcc ttccccgggt tattagcgcc
       61 gagtaagcgc ccggggaatg agtcattgcg cagatacgtc ccacacttct tgcagtaggc
      121 gtcaggattc tgctccgagg ctccacagtt cggacaaaac atagccgtgt atcttatgac
      181 gggttacacg cagcgcctaa cacgcttgag gtttgtcttt gaccgctttc tacagcaaag
      241 ctcgatcctc ttcattgaga tatgagacaa tgaatgggac aaaagacctc aactgtccag
      301 ttcaacttat cctttgagat tgaaactaat acaggccttc tgccccgaat aacaacggca
      361 gtcgataggc caggcccgtg aggacggggc tatcgactgc cgcgcctatg gcgctggccg
      421 atgcggataa tggaggacgc gttacgcgct gccgacgggg gccgttgcgc ctgactccag
      481 ctcggtgatg agttgcgtga gcatcggctc gccctgcgcc gcgtcgaggt agcccttgcc
      541 gagcaggcgc atcatcggcc gctgctgctg ccccggctcg ctacgcacct ccttgagcag
      601 cgcgcggcag aaggcgagga cgacctcggg cgcgaattgc tggcccgtgt tgcgtcgcag
      661 gtcttcgacc acttcgggga attcccggcg gcgcttgtag ggacggtcgg tcgtcatggc
      721 gtcgaaggag tcggcgagcg tgacgatctt ggcgcacagc ggtatctcgt cgcccgaaag
      781 cccgtcgggg tagccgcggc cgtccacgcg ctcgtggtga tagcgcgccg tgagcgggat
      841 gtcggcgtag gggtgtttga tgggcgagag gatttcgtag ccggcggccg ggtggcggtt
      901 cagctccgac gactgcttgg cgtcgatctt gtatggggcg ttgatgatgt cgcggtcaac
      961 gatgagcttg cccacgtcgt gaaggtagcc ggcaatcgcc atgccctcga cctcctcgtc
     1021 cgaccagccc aactcgcgcg cgatgatctc gctgtacttg cccacgcgct ccgagtgccc
     1081 ctgcgtgtac ttatccttgt agtcgatggc ggcggcgaag gcgcgcaccg tcccctgata
     1141 gatgccgcgc agctcctcgt agatgcgccg cgtctcctcg gcccggcgct cgatctcgcg
     1201 cagcagacgg tggttgtgca ggccgacgcc gatgtgccgg ccgagcgccc gcgccacgtc
     1261 gcgatcctcg accgtgtacg cctcgccgct cgccttctcg ccgaggagga tcagaccttc
     1321 gagccggccg tggacgagca acgggacgag cagcttgaca cgcgaacgct caagctccga
     1381 cagaaagcgc tcgctcagcg agacgcgctt gtcgtccgcg ctcatctcct cggcttcgac
     1441 ggagacggcc gcgccctcac ccccgacgag cgcctcggcc tccatcggcg agaaaggaac
     1501 ttccgacggc gcgccctcgg cgtggccgcg cgcggcgacg aagcgcatca caccagcgtc
     1561 cgcgtcgtac tcgccgaccg cgccgcgtct gacgccgagc gcgccgagca cgacctgaag
     1621 cgcggccgtg accatctcgt cgaagtcgct cgtgtcggcg atctcgacgc cgaggtcggc
     1681 gaccgccgag aaggcatgga tgactttgcg cagccggtca gacatatgtc tctcggagga
     1741 ggtcagtgtc cgcggggcgc gggggcggca aactcggcga gcgcggcctc ctcggagtcg
     1801 gccagcgcca cgcagcgcgt caggccgagc agctcgaaga gtttctggtt gtgacggttg
     1861 aggttggtaa agacgaggcg cgcgctgcga gcctcggcga tatccacgat gccgagcagg
     1921 atcgagacgc cgatgctgtt gacgagttcc gtgtcgcgga agttgatgac gacggtgcgg
     1981 gcgccctctt cgagccggag acggcactca cgttcgaggc gctgcccgct catcttgttg
     2041 acgtagtcgc cggcgtagac gacggccaca tctccggcgc gcgagggcgc gagcgcgggc
     2101 gagccgctgc gggcgctttc gttcatctgt tgtctcccct cttacttgcg caggtacttc
     2161 gtcattcgca ggcgtgtgcc gtcgtcaacg cgctcgaact cgacctcgtc catcagcgtg
     2221 cggatgagct tgaggcccca tccgcggcgt ccgggaatgc tgtcgttggc ctctcggccg
     2281 ttggtgtgtg cgagggcgta accgccggaa atggtcgcat cagggggggg agcgacgccc
     2341 cggctcgaaa cggtgacaac caatttatca ctttccagcc ggaaacgctg ataaattttt
     2401 cggtcggggc tgagggagtg ctccgaggcg ttgatgcacg cctcgacgag tgcgtgcttt
     2461 atctggctga tggcctcggg gtcgaactcc agccggcgcg cgatctgctc gacggtgtgg
     2521 gccgcgatca actccgtgtc gtcgcccatc ggcatgatca tctcgaactc gttggacgcc
     2581 gcctcactga cgtgcgcgcc cgcgccgccc gtgtcgagac gcgccgcgag gagttcgagc
     2641 tgcgcgcggc tggagccgaa cgcctcgcgc acattcaaaa gcttgttagc gtcttcggag
     2701 aagccctcgg gcgcgaccag ccagaggcgg gcgcggcgga agccgcaggc gtggacgacc
     2761 tgcgtcaggc ggtcgagcca gacctcggcg agcgcgcggc cggcttccag tttcgagtcg
     2821 atctcggcgg cgatccagac gacctcgttg tgctccgagt agggacttga gtcgaagccg
     2881 tgggcgacgg cgcagcgctc ctcgtcgatg acgagctgca cgggtgggtg gaacgaggcg
     2941 cagctcgcgg cgtggacgac ctgcggcaga cgcacgaggt cggtctcggc ttcaaggccg
     3001 gcggccactt cctcgggcgt cgcaccgcgg taggtgcgct ggaagcggtc gttgtgaaga
     3061 aggctggcgg gcacgcgctg gaggttgaag cgttccagta gttcgcgcag gccgagcgcg
     3121 gcctcgcggc ggtagtagcg cgccatcgtc tgcggggcgc gcttgagggc atcgaccagc
     3181 gtgttggcga cgacgagtgc gcgcggctcg acggcgacgt ggaggcggta gctggccctc
     3241 aggtagtcgt gccagacgag gctccggctc gtctcgacgt aagaagcgtc gaagccggcc
     3301 agctcgcgga cgtgcaggcc ggtcagaacc ttcttcagct ccgcgccctc gacgccgagg
     3361 cgacgacgcc atgcctcgac gggcgacttc ccttccacgt tctccgccgt ctcgtagagc
     3421 acgcgcacga gcgcgcggcg cgtgtcgatg gagggggcga tctcttcgag cgcggcgttg
     3481 aagcggcgat tgatgcgccc gccgaggagg tcgtccacgt agaggcgctg gaagtcgcgg
     3541 aaagtcccga ggtcgctcgc ggcgccctgc gcggtgcgga tgagcgcggt gatgagctgc
     3601 gggctgccct cgaactgctg caccatcagg tcgcgcgttt cgtcgctcag gttgacgccg
     3661 aggcgtgcgg ccacaacggt ggcgagcgcc cgcgcgtcct cgtcgctcag acgttcgagg
     3721 tgaatgaggc ccgtgtcggc cgcgcggtgc gcctcctcgg cgaggtgcat cacgtccgag
     3781 aagctgcgac gcaggccggc gaggacgaag ggcgtgtcgg tctgcgcggc catctgggcc
     3841 aactccgcgc cgaggccgcc ttccgcgtcg agttcggcgg cgaggtgtac gtcgtcgatc
     3901 atcacgagcg tgcgcgcgcc gtgcgcggcg gcctgctgcg gggcgccgag acagaggcgg
     3961 atgagcgcgg cctcatcctg ctctccccgc gcgcgctcgg aagcgctgac gatgcgctcc
     4021 acccagtcgt agtcggaggg cgtaatgagg tcgagcagcc cgccgagggt gggcggtgag
     4081 aagatgaggt cggggtcgcc gcggcggtgt gcgaccgtct ggacgacgaa tgactggagg
     4141 aaccgcaggg ccgcgccccc cgccccctga tcgcggcgcg agaaggagaa gtagacgggc
     4201 gaagcgccgc cgcgctcctg aaagagcgtg tcgaatgact ggcgcaggag ttcggacacg
     4261 ccggccatcg gcgcggcgag cacgaggagg ttgcgcggcc ccgacgtgtg cgcggcgagg
     4321 tcgcccacga ggcgcagctc cgcgccgcgc ccgacgaagt cctccggcgc gacgcggccg
     4381 aggatgcgcc gcttcttctg cgtctccccc gtcaccttat ttgtcttccg cgtcggcatc
     4441 cttcgagttc ttctccgcct ccgaccggta cgcgttcacg cagttcttgc cgcggctctt
     4501 ggcgacgtag agcgcctgat cggccgcgta gatgacactc gccggggtgt ccacgcccga
     4561 gccgaaggcg ctgatcccga tgctcacggt gacgtttccg gcgggctgcg ccttgtcatg
     4621 cgggaactgt gtgcgctcga tgcgccggcg gatgcgctcg ccgatgacga cggcctcaga
     4681 caagctcgtc tgcggcagca acacgcagaa ctcctcgccg ccgtagcgcg ccgccacgtc
     4741 ggccgagcgc agcgttgcct tcagacactg cgccgtcatc tccagcgcca aatcgccggc
     4801 ctggtggccg ttcaggtcgt tgtagtgctt gaagtcgtcg atgtccacca tcataaaact
     4861 catctgaaac tggtggcgct tcgaccgctc cagctcctcc gacagacgct cttcgagata
     4921 gcggcggttg agcaggcccg tgagcgggtc ggtgatcgag agcagttgga actgcgtcgc
     4981 cttctggtgc cactgcgcgc ggtcgagggc gagcgccatc tgcggcgcga tgagatcgag
     5041 gaggctgagg tcaacgtcgt cgtagttgcc gccgccggcc ttgtccgtga cgttaaggac
     5101 gccgaccttg cgccccgaga tgctgagcgg gtagctgatg aacgacttcg tcttgtagct
     5161 gcgctcggcc ggcgccggct tccaacccgc gagcgccacg tccgagacga cgagcggacg
     5221 gccctcgcgc atcaccgtgc cggcgacgcc ctcgcccatg cgcaggcgca cgtcctgcgc
     5281 cgcctcggtg cgagggccga ccatggcctt catcgaaagt tcgtcggccg cctcgtcata
     5341 gagcaggagc gagccgcgct cggcccgcag catctcggcc gactggcgca ggatcatctg
     5401 gtacgcctcc tcgggcggcg ccgagttgat cctctcgacg aaggcgcgca ggtgtgtggc
     5461 ggtgcgcatc cgccgctcca gctcctcgcg cagacgcagc acttcgagcg gcaacgcgat
     5521 ctcgcgacag aaggcggcga cggctctccg ctgctcctcc gacaagtgct tgtcgccgac
     5581 taagagcgct cccttcatct cctcgccgac agcgagcggg aagagttcga cggcgtcctc
     5641 gtgacgggtc gccgtgcgcc cgcccgcggg gacggcgata ctctcgccgt gcgtggccgc
     5701 ctgaagcagc ttgatctctt tagccttgag cgagacgcgg ggcggctgcg cgcggaactg
     5761 ccccgtcgtg tagaccgacg taaacttttc gtcggcgcgc agcatcagcg cgacggactc
     5821 gacgccgtgc gactcgcaca atttcctcac ggccttctcg cacgcctccg ccagcgacgt
     5881 gttagaggcg aatcccctcg cctgcgaact cggcgcggag ccgttggcgt gcggcatcgc
     5941 ggcgggggcc ggcgctgctc cgcgccgcgc gccgcgcgct tcgctcgcct cgtcaacccc
     6001 attcgacttt tcggcctccg gcgcttccct cttgcccacg cccgcgtcgg ccttccccgc
     6061 ggctggcgcg cccacgcccc tgccggtcgc cgcggatttc cgtgagccgc gcgcgccctt
     6121 cgggaactct tcggccgcct cgcggaggcg gacggccaga tcagcgaggt cttgttcgag
     6181 cgcgaagatg acgttgcgga agaggtctgg gctgagcagg tcggagaggt cgccgctacg
     6241 catgcgctcg gcgagcacgc ggtagtcgga gctgcggagg aaggcgcggc cggcgatgac
     6301 ggcgagacgc tcggcggcgc cgaggtcgag gggtgcggcg aagcagtgga ggccggcgtg
     6361 gcagcgatag tgagccgggc cggcggcctc ctgtgcgcgc tcgaaggcgc ggccgcagaa
     6421 ggggtcgcag aggtgcgcgt gtttgggcga cgactggaag gcctggcaga tggagttgtt
     6481 atgtgagacg cgaagcgcgg ggggctggcg accctcgact aagagtaggg cgaggcccga
     6541 agccgctgcc agcgactcct gcacctctgc ccagccggtg gggacggtcg cggcagacgg
     6601 attttcggca tgctctgggg cggaagctgc tttatccatt aaatttgcgg aacactttgg
     6661 gggtccgcga gtgcggcctg aaggcgggtc tttccgaaga ccttggggag gcgcgtccgc
     6721 acccacgcct ttcagacgga aaggacggcg ggcgcctggg acctccgccg tcccacagcc
     6781 gcagtatagg tgtccgcacg taaacaagac aagagttatc cctttaaaag acttaaccct
     6841 cgcccgggga acacggaggc cgaagcatcc gcaagccata ggaaacggtc aatcggaggg
     6901 atatttcact gcgcgatgat gcggccgggg tttctcaggc ccggagcatc gccgggagcc
     6961 acccgctcga tgtccggctg gtcgggactg atgcgtatac aggcggcgaa atggttcggc
     7021 ttgatctctg ccagaggggg cacgacctcc ttacactcgc ggatggcgta ggggcagcgc
     7081 gtgtggaagc ggcagcctga cggcgggttg atcggcgagg gcacgtcgcc ctgcaagacg
     7141 atgcgttgtc gtccggcctc ggcctccggg tcgggcacgg gcacggccga aatcagcgcc
     7201 cgcgtgtaag gcatcaacgg gtcgtcgtag atggtcttgg ccccggcgac ttcgacgagc
     7261 ttgccgaggt acatcacggc gacgcggtcg gagacgtggc ggatggcccg caggtcgtga
     7321 gagatgaaga ggtaagtgag attgcgctcc tgctggagcc gctccatcag attcatgatc
     7381 tgcgcctgaa tggaaacgtc gagcgccgag atgggttcgt cggcgacgat gaagtcggga
     7441 tcgacggcga gcgcgcgggc gatgccgatg cgctggcgct gtccgccgga gaactcgtgc
     7501 gggtaacgct tgacgaagcg cgggctgagg cccacggtct ccatcagctc gcgcacgcgc
     7561 tcgttgcgct cgtgccttcc ttgcgcgagg ccgaacgtat cgaccggctc gccgatgatt
     7621 tgccccacgg tcatgcgcgg gttgagcgag gcgtaagggt cctgaaagat catctggaga
     7681 tgacggcggt gcttgcgcag ctccttttgc gaaaggtgcg cgaggtcgcg tccgcggaaa
     7741 aagacctgac cgcccgtcgg ctccgtcaga cgcagcagcg cgcggcccag cgtcgatttg
     7801 ccgcagcccg actcgccgac gaggcccaaa gtctcgccgg ggaagatgtc gatggagacg
     7861 ccgtccacgg ccttgaccac gcggcggacg gaggagccgc ccgtcaactt gtcgaggaag
     7921 gtgccgccgc cgaggtcgaa gtgaactttg agttcgcgta tctcgatgag gggttcggcg
     7981 ccctcggccg ggaagagggt cgggggcatc cccgccgccc cggtctgatc gatgccctgt
     8041 gtgctcatca gcgtgtgtcc ccctgcggcg gcgtcgttgt ggtcgtcgtg tcatcgcgcc
     8101 ggtcggtcgt cgcgcccggc ggggcggccg aggcgcgtcg gtcgcgctcg tcgcacgaag
     8161 cggcgtagac ctcttcggcg aagtgacaga gcgagaagtg ttcggcgttg agttggacga
     8221 agggcgggaa ctcctgccgg cagatgtcct cggcgcgctc gcaacgcggg gcgaaggggc
     8281 aaccgggcgg caggtgcgcc acgtccggcg gcagccccgg aatctggtag agcttgccct
     8341 gctcggaggt cgggtcgggg acggagcgga gcagcccctt cgtgtagggg ttgccggggc
     8401 aggagaacag ctcgcgcgtc ggcgcctgct cgaagacctt gccggcgtac atgacgatga
     8461 tgtggtcggt catcccggcg accacgccga ggtcgtgtgt gatgaggatg acgctggtgc
     8521 ccgtgtcggc cttgagctgc ttgatgagtt cgagaatctg cgcctgaatg gtcacgtcga
     8581 gcgcggtcgt cggctcgtcg gcgattaaaa gttccggctc gcacgaaagc gccatggcga
     8641 tcatcacgcg ctggcgcatg ccgcctgaga actcgtgcgg gtagctatcc acgcgcgtgc
     8701 gcgcgtcggg gatgccgact atttccagca tcttgatggc gtgctcgtag gcctgatctt
     8761 tcgtgtgccc gaggtggagc cgcgtaatct ccatcaactg cttgctgatc cgcatgaagg
     8821 gattgagcga agtcatcggg tcctgaaaga tcatgctgat gcgcttgccg cgaatcttgc
     8881 gcacctcgtc tgtggacagc gtcaggatgt cctgaccgtg aaagatgacc tcgccggaga
     8941 cgattcttcc gggcggctgc ggaatgagcc ggatgatcga gaggttggtg acggacttgc
     9001 ccgaccccga ctcgccgacg atccccagcg tctcgccgcg ctccaaatcg aacgtgatgc
     9061 cgtcaacggc cttgaccacg ccgtcctccg tgtcgaagta cgtccgcagg tctctgaccg
     9121 agagtagggt gccgttgttg ctgctcatag gcttcgcgtg ataagtgatg agtgataagt
     9181 gataagaaaa acccgcctgc tctttcttat cacttatcac tcatcactca tcacctctta
     9241 aaattttctc atctgcgggt cgagcgcgtc gcgcaggccg tcgccgagga agttgaggga
     9301 gaagagcgtc agggccatcg tgacgccggg aaagatgagt tgccacggga agatggcgat
     9361 gttctggatg ccgagcgagg cgaggctccc ccacgaggcg tagggcgcct gcacgccgag
     9421 gccgaggaag gagagaaacg cttccgagag catcacctgt gggatggtca gcgtcgcgta
     9481 gacgatgacc ggccccagca cgttcggcac gatgtggcgg aagatgatgc ggggcacgtt
     9541 gacgcccgtg gcgcgtgcgg cgagcacgaa ctcctgattc ttcagggaga gcacctgccc
     9601 gcgcacgatg cgcgccatcg tcagccagga gatcgcgccg agcgcggcga acagcaaaaa
     9661 gatttgtttg agcggcgtct ggctgccgaa gaaggcgagg agcacgatga cgatcatcat
     9721 gtaggggatg gcgtagagca cgtccacgac gcgcatcatc acgtcgtcaa ctttcccgcc
     9781 gagatagccg gccgttgcgc cgtagccgat gccgatgagc agcgaaacga tggtcgagat
     9841 gaagccgacg gccagagaga tgcgcccgcc ctgaaggacg cgcgcgagga tgtctcggcc
     9901 ctcgctgtcg gtgcccatcg ggtgcagcca cgagaaggtg ccgtcggggg cggtaaacgg
     9961 cgggaacgac ttgacgagac tggagtcggc cggaaagtcg tcgtagtcga agccggcggt
    10021 ggccttgatg atgggcgggc cgatgatcga ggcgaggatg atgagcacga cgacgatcat
    10081 gccgaagacc gcgaggcggt ttttcagtag acgccgccac gcgtctttcc agagcgacgt
    10141 gccggcgacc gccacgacgg cgctgcccgg ctcggcggtg cgcacgtcgc cctcgcccac
    10201 ggccagctcc ggccccgtgt gaatctgatc gccctttcgg tggcgaatgt ccggctgctg
    10261 gggcttgccc agcggggtaa aggcaggctc cggctcggac gcgccgtgcg gcgtctccga
    10321 acgtccttcc tccgtcggca ctctcggcgt tctctcgtcg ttcataattt tcagtaccgg
    10381 atgcgcgggt cgatgaacgc gtaggcgatg tcaaccagca ggttgaagat caggatgacg
    10441 atggagatga aggcgacgat gccgataatc agcggctcgt cgcggttgag attggcgttg
    10501 atgaaatagt tgcccagtcc cggcatggcg tagatgcgct cgacgacgac ggtgcccgcg
    10561 ataagcgccg cgagcgccgg ccccgtgtag gagacgacgg gcagcaagcc cccgcgcatc
    10621 gcgtgtcgcc agatgacctc gcgctccttg aggcccttgg cgcgcgccgt gcggatgtag
    10681 tcggagcgca ggacttcgag catccccgag cgtgtcaggc gcgcgatgta ggccatgtag
    10741 atggccgaga gcgtgaagac cgggagcagc aggttccaac tggggaagcc gccccagcgc
    10801 gccggcggga gccagaagag ggtgagcgca aacaccatca cgaggatcgg cccgagcacg
    10861 aagttcggca ccgagatgcc gagcatggag acggccatcg aggcgtagtc gagccgggag
    10921 ttctgtttga gcgccgcgag cgtgcccgcc gtgatgccga cgacgagggc gaggaggtag
    10981 gctaacacgc cgagtgtggc cgagacgggg agcgtccgct tgaggatgtc gttgacggac
    11041 tcgctctcgt acttcagcga gttgccgaag tcgaggcggg cgacgctgcc gatcatccgt
    11101 ccgtactgga cgtaccacgg cttgtcgagg ccgtacttct cgcggatgtt cttctcgatg
    11161 gcggccggca gcttcttctc gcccgagtag aagttgcccg gcgcgagccg gatgagcgcc
    11221 cacgtgagcg tgaccacgat gagcaccatc ggcacggtga cgatcaggcg gcggatgatg
    11281 aatgggaaca tcgcttcccc tcttccagat caaaactcaa gggaatgaaa tatgaatgga
    11341 caggatcaac aggacgaaca ggacaaaatg atttgaaatc tcaaatctga aatcagccca
    11401 ttcttcatcc tgtccgtcct gttaatcctg tccatatgtg taattcttat tgcggcaaac
    11461 gcgccatctc gcgggtcgcg atttagtcgg ccagactctc cgacttcatg tcgggcatct
    11521 gcttgtccca cttggcgcgg tcgtgctcga tgtagacgta cttccacgcg tggagcgttc
    11581 cggggttcgg gtagaggccc ttgacgtagg gcttcttcat ccagctcgtc gccggggtga
    11641 cgagaggcag caccggctgc acgtcgagca ggtacgcctc ggcttcggcg agcagcgcgt
    11701 agcgcttggt ctggtcaggc tcgctgttgg ccgccttcag cttggcgacg tacttctggt
    11761 cgatccagcc cgtgccgttg tcgtggccga gcgtgctgaa gagagcgagg aacgtgtagg
    11821 ggtccatgaa gtcgccgacc cagccggcgc gggcgaagcc cttgtactgg agcttggagc
    11881 gcatgtcgag gaaagacttc cactcgacgt tcttgagcgg gatggtcatc cccagatgtt
    11941 gcttccactg cgcctgaatg aactcggcga ccgcgcggtt cgactcgctc gtgttgtagg
    12001 tgatctccac ctccgaaacg gggaacttcg acgggtcgaa cttgcccgac gcgtctttgt
    12061 agccggcctc ggcgaggagc tgcttggcgc gctcggggtt gaagtcgtcg cctttgggct
    12121 gcgggtagcc ggggtagatg ccttcgggcg agaaggccgt cagaggcttg acgacgacgc
    12181 ggtagtcggc gagcgccttg cggtcgacac ccatggcgaa cgccttgcgg acgcgccggt
    12241 cgttcatcgg cgccttcgtg gtgttgatct ggtaatactc gatggcgttc tcgggcgcgt
    12301 ccatgtagtc cttcatctcg cgcaccccgc tcttgagcca cgcggccggc accgtgtggt
    12361 tgtaggtggc gtccacgtcg cccgccttgt agaggttgag catcgtcgtc tgctcttcga
    12421 gcgggtagaa gcgaatctca tcgaggcgga cggtggcggc gtcccagtac tgctgattct
    12481 tgacgacgtg gacttcgttg tagggccggt gcgacttgag cgtgaaggcg ccgctgacga
    12541 cgatgttctc cggtctcgtc cacgccacgc cgtgacgctc gaccgccccc tcgtggatga
    12601 cgcggaagaa ttgatgcggc agcaggccga tgaaataggg cgcgggctgc atgagcgtca
    12661 accgcagcgt gtggtcgtca accgcctcga cgcccaggtc ttccggcttg acgggcacga
    12721 actccttgcc ggccagaagg ccctgaagct tcgggttctc cttgaacagc ttctcgcgct
    12781 ccttcgcctc cgacggcacg acgaggcgcg tgggctggtg catgtattta tgaaactcgg
    12841 tgtcgggtgc ggcgtcctcg cccttcttgg atagctcggc gcggtgcagc gcctcggggt
    12901 cggtcgtggc gggcggcgcg gcggcgggtt cttcttcctt cttctgggcc gcagcttggt
    12961 cgcccggctc cttcgtcggc gcggcgctcg ttccgtcggc gggcttcacg gccgcgcccg
    13021 ccgcttcggc cgccgcggcg ggggccttct cggcgggcgg cgcggcttcc gccgcagtca
    13081 cgaaccggcc cgtcgccggg tcgcggacga acgagccgcc ctcgttgacg ccctgcgcgt
    13141 acttgatctc gtaggcgaga taggcgctgc gcgcggcgag ttcgggctgg aggccgcggc
    13201 ggaaggagta gacgaagtcg cgtgcggtga tggggtcgcc gttggagaac ttggcgtcgc
    13261 ggcgcaagtg gaagacgatc tcggtagcgt cctcgtcgag cgtccaacgc tcggcgatgg
    13321 ccgggatggg ctgcatcgtc ttcgggtggt actcgacgag gccgtcgtag agcgccatga
    13381 agatgcgcgt ctcgggctgg ccggagctgc ggtgcgggtc gagcgactcc ggctcggagc
    13441 cggagatgta gcgcaacgtc tgccccggcg gcggctcgat cttgccgaag aactccgact
    13501 tgctcgcctg cgcggcgcag ccggcggcga cggcggcgag tcccagcaca atggaggcga
    13561 ggagggccgt gacgatgcga tttctaaaca tggcgtatta actcctgttg tctcggatta
    13621 cttctaaacc gcacgggacg ccacacgggg agatgtgtgt gggctttcct caaccccgcg
    13681 cgcagttact aaggacgaac gcgctgttcg gagcggagtg ctaaggacgg gggagcggct
    13741 cgggggcttg gcttgctctt gctttgtttt tgcgggcctc aaattatcac gtcacccgcc
    13801 cacaattcaa gacgcttcaa ccggccgggc ctgttgtcga gatgcccgcg gggcaacgcc
    13861 ccgttgctca aacgcgccaa gttttacaag cccgacacag tggcggcggg cggcgagacg
    13921 cccgtcccct cgcccggttt ccacccggtc tcgatccgca cgtacttgag cgagggcgcg
    13981 tcgaggaggt tcgtgtcgaa gcccgcgacg tagggcttga cgagcgcgaa cgaggtggcg
    14041 aagtagaccg ggatggcggg caacgcccgc agcgcctcgg cctccgtcgt aatcgccgcg
    14101 accgactcgc gcgccgcctc ctccgtcggc ggtgctttgg acgagtccga cgcggcctgc
    14161 gaagcctgtg cttctggcgt cgagtcggtc tcgtccggcg tggtcgccga tagccccgcg
    14221 gcgccgaaca tcgcgagcag gttccgctcc tcgtccggcg tctgcatgac gatgctccgg
    14281 cgcgcgatgt cgaagtcgcc ggcggcgagc gacgactcgt agtgctccca atccttcaac
    14341 gtcacttccg tctcgacgcc gaggacttcg cgccacattc ctgcgacggc ctgcgccagc
    14401 agccgctgcc gctcgttgcg attgacgagc aggcggatga cggggaactt cgcgccgccc
    14461 gcgtaacccg cctcagccag caactccctc gcgcgcacgg ggtcgaaggt caacggggcg
    14521 gccctttgct cgtcggaaga tgtcttcgcg gcttccgacg ctttcgcgcc ttctccactt
    14581 acccctgcgc tcgcttccgc gccgggcaga aagcgtgcgg ccgggagcgt ggcgccgccc
    14641 aacgtgtcgg cgctcaggcg ttcgcggtcg agcgcgagcg cgagggcgcg tcgcacgcgc
    14701 aggtcgtcga agggcgcgcg cgtcgtgttg aactggtagt aggtcagcgc gccgaagatt
    14761 tgccggcgga agtcctcgtg cggcgcgagg agcttcaagg cgagcggctc gaaggcggcg
    14821 ttggtgacgg cgtccacttg tccagcgcgg tactgcgaga gcgcctcctc ggcgtcggcc
    14881 gaggcgacga aatgcacgcg ctccagttgg acactcgacg cgccccagta gttctcggcg
    14941 cgttcgagca cgacgccgcc ctcggcgcgc ccgcgcagac gaaacgcgcc gttcgtgatg
    15001 agcgtctttt cgtcctccgc gcccagcgcc ttgccgcgtc ccgcatgcgc gggcgtgaga
    15061 tcggcgtgcg cgccaagttc gtggacgggt ctgaagacgg ggtgcgcgac gagcgccggg
    15121 aagagcgcgt cgggcctctt gaggcgcacg acgagggttt gcgcgtccga cgcacgcgcg
    15181 ccaaagtccg gcggcgcggg cacgggcgtc ggcagtgccg ccgcggcctc ccccaatttc
    15241 ctgaattgct gccccatgtc gaagccgaaa aacttcggct cgttcacctc ggacgggaca
    15301 acaccggcgt cgggggcccc ctcgatgttt cgcatgagac cggcgtgcgg cgcccgctcg
    15361 ccgagctgga gcgtgcgctt ccacgagcgc acgaagtcgt gcgcggtgac cgggtcgccg
    15421 ttcgaccagc gcgcttcggg gcgcaaacgg aaagtccaga cgcgaccgcc ctcgcccgcc
    15481 tcccatgcgg tggcgacggc cggcaccggc gcgagcgtgc gcggttcgta gtcggtcagc
    15541 ccctcgaaca tggcgcgcac cacgtcggtg tccggcggcg aggcggcgat ggccgggtcg
    15601 aagacgcgcg gcaacccgcc gtcgctccaa cggaactcct gcgccgcggg cacggccacg
    15661 cggccgtaga aggccgcgcc cctctcctgc tcgaagcacc cctgagacga aagcatcaac
    15721 gcgaacgcga gcgcaacgag cgggcgagcg cgacggccgc gaccgatggg attgaagatg
    15781 tgaatgactt gcttcagcat ttaccctgtg ctgctattaa ctaagtgcgt ctgatgtttc
    15841 tgtatgggca gggacaagtc cgctgcccac atcttgggaa gcaatttaca tggatagaca
    15901 ggataatcag gatgaagaaa tttgaaattt gaaatctcaa atcttgaatc tgatatcagc
    15961 tctttcttca tccttcacat cctgcccatc catgtaaatt tctttcgcgt ccggcgggcc
    16021 acggacaagc cctgccccta caattttatt ctagctctcc ccggccgctt tcttcttcgc
    16081 aagtttacgg agcgccgcgt acacctcttc ggttcgcctc cgcgtgtcct cgaagccctg
    16141 cgacgtgtcg atacggaagt cggcgtagcg caacttctca ccttgcggca tctgcgcggc
    16201 gatgcgtcgc tcggccgact ccgccgacat cccgtcgcgc tcaatcaaac gtcgccgctg
    16261 aagctccggc cggcagtgga cgacgatcag cttctcgaaa cgcttgtacc cgcccgactc
    16321 gatcatcagc gcggcatcga cgacggcgac gccctccggg tcaacctcct cgaattcccg
    16381 tagccgctcg tcctgcgccg cgatgatcag cggatgcaga atcgagttaa gcaactcccg
    16441 cagcttctca tcgttgaaga cgatgccgcc cagcaccgcg cggtcgagcg ccccttccgg
    16501 cgtcagcacc ttctgaccga acgcctcgac gatgcgacgc aagccctccg tccccggctc
    16561 gactacttcg cgcgcaactc tgtcggcgtc gatcacgtga cagccgagtt cccccagcac
    16621 gcccgacacg aacgacttcc ccgtcgcaat cgaacccgtc agccctacac gcaacatgcc
    16681 tcataaggca aaagtaaaaa ggcaaaaggc aaaagtgata tagagagcaa cagattgtgt
    16741 tgccagcgtc ggacgcccac ggtgctctta tccttttgcc tttttacttt taccttttgc
    16801 cttcctccat tccccgcctg cggcgcgcgg cctcgaccgt gttcctcatc agcagggcga
    16861 cggtcagcgg cccgacgccg cccggcaccg gcgtcagacg cccggccacc ttctcgacct
    16921 cggccggatg aacgtcgccg acgatggtgt agccgcgtct gcccacaccc tcaagacgct
    16981 tctccgcgcg cgcgccgaac aactcccgaa tcgtcgcctc gtccgacaca cggttgatgc
    17041 cgacatcgat gacggtcgcg ccctctttaa cgtgctcgcc gcggataaag cccggccggc
    17101 cgatggccgc gacgaggatg tcagcctcgc gcgtgaccgc cggcaggtcg cgcgtgcgcg
    17161 agtggcaaat ggtgacggtc gcgtccgctt gcagcagcaa ctcggcaacg gggtggccga
    17221 cgatgtggct gcggccgacc acgcacgcgt gcgccccgcg gatcgtgagt ccctctcgca
    17281 cgagcagctc cagcacgcct gccggggtac acggcacgag cgccggccgg cgcagcgcga
    17341 ggcggccgac gttcgtgggg tggaagccgt ccacgtcctt cgccgggtcg attgactcga
    17401 tgacgaccga ctcgtccaac cctttcggca gcggcaactg tacgagtatg ccgtccacgt
    17461 catcgcgccg gttcagaccc tcgactaact ccaacagctc gccgcccgtc gtctccgccg
    17521 agagctgtcg gtgctccgat ttgattccca gctcctcgca cgccctgacc ttactccgca
    17581 cgtagacttc cgaggccggg tcgtcgccca cgcggacgac cacgaggcac ggcacaacag
    17641 cgtgctccgc ggcgagtcgc gccacgtccg cgccgacctc ccgcttgatc tcggcggcca
    17701 cgcgcgcgcc gtcgagcacg ctcgcgcgcc ccgtctcgcc ctctctgctc ccgctgctca
    17761 tcctgtccgg catcctaaca caaatccgcg agggcacacc ttcggccgcc tgttcggccg
    17821 gcgctcgcgg ccgtggttgc gaaggcacag gatgtgtcgg acaatccgac acatgaaacg
    17881 gcgtcaatcc gacggcagca ccagcgggaa ctttttcagg ccggcggccc tcttcgcgtt
    17941 gctgagtgtt ctcatcctcg gcggctgtgt tgcgtcgaag ggtcacggcg aagtgcggcg
    18001 ttacgacttg aagggcaagg tggtctccgt cgagcgcggg cgcggcgagg tcgtcgtcga
    18061 acacgaaacg ataccggggc tgatggacgc gatgaccatg cccttcgcgc tcaaggacaa
    18121 agacgcgctc agtgtcgtcg agccgggcga ccgccttcag gccacgctcg tcgtcgccga
    18181 cagcggcggc ttctggctcg aaagcccgat gattacgaag gccgccccgt cgggcgaatc
    18241 tcccgcgaac gaatcgtcgg gggggccgaa ccccggcgac gttgtgcccg acttttcgct
    18301 cgtcaatcaa gacgacaagc ccgtccgcct cggccgctac aggggccgcg ctttgctcct
    18361 caccttcatc tacacgcgct gcccgctcgc cgactactgc ccgctgatga gcgaacgctt
    18421 cgccgccatc agccgcgagc ttgccggcga agcgtcattg aaggacaagg ttcaactgct
    18481 gagcgtctcc atcgaccccg cgcacgacac gcccaaagtc ctccgcagct acggcgcggc
    18541 gcacacgcag aagtatggcg gcgagacgtt cgcgagttgg gagttcgcca ccggcgaccc
    18601 cgaggaggta aagaagctgg cgcagttttt cgggctggaa tacttcgccg agaaggatca
    18661 gatcatccac tccctgcgca cggccgtcat tgaccccacg ggccgcgtcc acaaaatcta
    18721 tcgcgggaac gagtggcaac cctcggaagt tttaaacgat gtgcggacac tcttcaggga
    18781 gcatcattga cgggaaggca aaagttaaaa aggcaaaggg caaaagtgag gaaagcgtct
    18841 cgcctcttca cttttgcctt ttgccttttt acttttgctt tactagtgcc cgacaggtat
    18901 ccgttgatac cgcgatttcc cttccgcgtc ctcccaggaa atatccagca cgtaaggtct
    18961 gactccgccc tcgactattt tgagacgctc cagctcgtcg taacgcgccg ccgccggttt
    19021 accgtcgatc cacgtcttgt gagataagac gaccttctct ttcggctgga tagacttgac
    19081 ggtctcaaat tttatatgcg ctcgtgaagg gccgccgccc ggagtctctt cgtccttcag
    19141 aaagacggtc agcacctcga cgcacttgag tgtcacatca gtcgtattca tcaattccaa
    19201 acggacgttt ggttcgccct cgacgaattt ggcggtaaat gattcc
//
LOCUS       DASVES010000025        10404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1140293, whole genome shotgun sequence.
ACCESSION   DASVES010000025 DASVES010000000
VERSION     DASVES010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140293"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VF240_01000"
     CDS             <1..436
                     /locus_tag="VF240_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7173847.1"
                     /translation="RGGSLAGLSRRQKTMKQSIAHIALVVDDYDEAIKFYTEKLNFTL
                     LEDTAQSETKRWVLVAPKGAEECSLLLAKGVGDEQRSRIGNQTGGRVFLFLKTDDFWR
                     DYKNMLSQDIVFVREPKNEDYGTVAVFKDLYGNLWDLIEFKK"
     gene            478..1281
                     /locus_tag="VF240_01005"
     CDS             478..1281
                     /locus_tag="VF240_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX7173848.1"
                     /translation="MNERTTKPTREGDSPPALSIIIPTLNEARSIGQTLDAVMGAQGA
                     VEVIVVDGGSVDGTAEIARGRGARLVTSGRGRGAQMHAGACAARGSALWFLHADTRAS
                     AGCAEAVAGALRDPEVVAGNFSVRFDGDGRAARFLTWLYPRLRLLGLCYGDSGIFVRR
                     EAYQRTGGFRPFPIFEDLDLVRRLRKTGRVVYLPDIVTTSSRRFEGRSFALTFARWAF
                     LQVLYWLGVHPRTLGRMYAPVRNAGGKSKRDDDDPAGARGVACSDGRGQ"
     gene            complement(1261..1998)
                     /locus_tag="VF240_01010"
     CDS             complement(1261..1998)
                     /locus_tag="VF240_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX7173849.1"
                     /translation="MLFFIPHPSSFILALMRPVCIVMVKAPRAGEVKTRLVPPLSGAQ
                     AAGVAACFAQDTVAALRRVVPEVIVAYAPADGRDVLEALLPPGNLFWLEQRGEDLGER
                     LSTAVTHAATLGHSPVIVTGSDSPTLPTSFVESAIASLTAGESDIALGPTEDGGYYLV
                     GLRQPVAGLFRNVAWSTPRAHRQTSQTAARLNLRVLDLPHWYDVDTFSDLLHLRDEIF
                     TSEEARERAPATHRWLLEHDSLLSAPV"
     gene            2079..2408
                     /locus_tag="VF240_01015"
     CDS             2079..2408
                     /locus_tag="VF240_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEX7173850.1"
                     /translation="MAYYEEDDLQRFSEVGKHRPDLFEKFMAWYEACQEDGALSRREK
                     ALIGLAVAHALQCPYCIDAYSQSCLEAGSNMEQMTEAIHMASALRGGATLIHALQAHN
                     SVNKVSM"
     gene            2408..3427
                     /gene="arsS"
                     /locus_tag="VF240_01020"
     CDS             2408..3427
                     /gene="arsS"
                     /locus_tag="VF240_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEX7173851.1"
                     /translation="MSKALPVIPEGPTSRARATVSFDEKLAAHGTLLQRAEGGVETLQ
                     VNVGKLCNQACKHCHVDAGPKRTEVMKRETAEQVIAAVVRFRLPTLDITGGAPELNPS
                     FRYLVAEARRSGAHVIVRHNLTVMFEPGQDDLPEFFREHEVEVVSSLPYFLEQQTDAQ
                     RGAGVFQKSIEALRRLNAVGYGGEGSGPVLNLVYNPTGAFLPPAQESIESDFKRELWT
                     RYRVTFDRLYTITNMPIRRFLDYLRRSGNEERYMRKLVEAFNPSTVEGLMCRNLVSVD
                     WTGKLYDCDFNQMLELGVPEGLPRTIADFDPAAFAGRRIATGAHCFGCTAGSGSSCGG
                     AVAAS"
     gene            3429..3809
                     /locus_tag="VF240_01025"
     CDS             3429..3809
                     /locus_tag="VF240_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173852.1"
                     /translation="MTERTNSAKRGLVFVYNADSGLFNAVSDAAHKIFSPRTYQCNLC
                     ALTHSAIGMRREWKQFLDGLNVPLEFLHADELKARDGVAGVPLPAIFKRGDEGVEVLI
                     DAESINACRTSDDLKQLILNQLSA"
     gene            complement(3877..4554)
                     /gene="msrA"
                     /locus_tag="VF240_01030"
     CDS             complement(3877..4554)
                     /gene="msrA"
                     /locus_tag="VF240_01030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7173853.1"
                     /translation="MTRHLSALLALFLVGATLACNAAVATTTPSAGIPDPAVDAPLAN
                     VKGEQVAVLAGGCFWGVEAVFEHVKGVTSVVSGYSGGDAKKADYESVSGGNTGHAESV
                     RITYDPSQITYGRLLKVFFSVAHNPTELNRQGPDTGTHYRSAIFYAGEEQKRIAESYI
                     VQLNGAKIFKRRIVTQVAALKSFHEAEAYHQDYAVHHPDQPYIVIHDLPKVEALRKQL
                     PELYVAK"
     gene            4908..5096
                     /locus_tag="VF240_01035"
     CDS             4908..5096
                     /locus_tag="VF240_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173854.1"
                     /translation="MSSNTRPLAIVTGASAGIGYELAKLCADNDDGSPVAAAQPGIEE
                     AAQTFRQHETSSLLTQGQ"
     gene            5121..5972
                     /locus_tag="VF240_01040"
     CDS             5121..5972
                     /locus_tag="VF240_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7173855.1"
                     /translation="MGYITTRDNTKLYVKDWGRGRPVILLHGWPLSADSWDDQAVAIA
                     DAGFRAIAYDRRGFGRSEQPWEGYNYDTLTDDLAAVVEETGATDVSLVGFSMGGGEVA
                     RYMSRHGGKNVAQAVLVSSVVPYMLQTADNPDGVPQATFDQMTAGMKQDRAHFFAGFF
                     KDFYGVGLVSQPVSDELLETSRGVAMQASLKATLDCAKAFATTDFRPDLASFSVPTLI
                     IHGADDKTVPIDATGRASARGIPHAKLIEYDGAPHGLFATHKERLTSDLLAFLGQSVG
                     NAAHGSR"
     gene            6099..7187
                     /locus_tag="VF240_01045"
     CDS             6099..7187
                     /locus_tag="VF240_01045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX7173856.1"
                     /translation="MKITSTAICGSDLHLFDGFMPTMEEGDILGHEPMGVVAEVGKEV
                     KNLKVGDRVVVPFTISCGECFFCQKEMYSLCDNSNPNAKIARAAMGHSPAGLFGFSHM
                     LGGYAGGQAEYLRVPFADVGPLKIENDSLTDEQVLFLSDIFPTGYMAAENCGIEKGDT
                     VAVWGCGPVGQMTIQSAWMLGAERVIAIDRVPERLAMAAKHGKAETINFQDEKVYDQL
                     IEMTKGRGPDRCVDAVGAEAHATGSIDSVLDAAKTAVYLGTDRPHVIREAIMCCRKGG
                     TVSIPGAYVGVMDKLPLGALMNKGLTIKTGQTHVQHYNKPLLEKIEAGEIDPSFVITH
                     RVKLDDAPKAYATFRDKEDGCIKVVLKP"
     gene            complement(7212..9113)
                     /locus_tag="VF240_01050"
     CDS             complement(7212..9113)
                     /locus_tag="VF240_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7173857.1"
                     /translation="MTMHILSVEAVSKDYGLRPLFENVSLGLNDDDRVGVVGVNGSGK
                     TTFLRLIAGEERPDAGRITLAEGVSIGYLPQNPPYVADETVLDAVFAASDAKMKLLLD
                     YERACRELSTKSGDEQRALNRVSELAHRLEASGAWEMETNARMVLSKLGITDTNARMG
                     ALSGGQRKRVALAHALIERPSLLILDEPTNHLDAETISWLEGYLERYTGALLLVTHDR
                     YFLDRVATRMLEIDRSAVQAFDGNYAYYLEKKEEQESARAAEGQKREMLIRRELAWLR
                     RGAKARTRKSKARVDRAESLIAQPKEAARAVLDMSVGSKRLGKKVLELSNITKSYGGR
                     VLIEDFSRTLKPGERIGVIGPNGAGKTTLLEIIAGRVAPDTGFVEVGQTVRFGYYDQE
                     SRALDEQERVIDYIRAVAERIETADGTFITAGQMLEKFLFTGAMQYAPIGKISGGERR
                     RLYLLRVLMGAPNVLLLDEPTNDLDIQTLVTLEDYLDGFGGNLVVVSHDRYFLDRTVE
                     HIFRFEGEGRLREYPGNYSAFLEAHRKQERDGAPTVEATAVRPPVRQRVAPPSANRKL
                     SFKERKELEATEARIQAAERREVEIETELTLNSSNADLVHQLYQEREELTEQLARDLD
                     RWAELAEHA"
     gene            complement(9328..>10404)
                     /locus_tag="VF240_01055"
     CDS             complement(9328..>10404)
                     /locus_tag="VF240_01055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7173858.1"
                     /translation="YGLLRRDVEMLMHYEFNYLILDEAQAIKNPLAETAKAVRLLRGA
                     HRLTLTGTPVENNTLELWSQFAFLNPGLLGNAEYFREEFATPIERKGDEEAAQFLRKM
                     IYPFILRRTKDQVAKDLPPRTERQMVCDMEPAQRKLYDERRDYYRALLLKLIEDEGIN
                     NARFRVLEGLLRLRQICNHPRLAVKTYRGGSAKLELLMETLSTLHAEGHKALIFSQFV
                     QMLKLVEGELKKRRIPFSYLDGQTRDRQERVDAFQSVPSIPFFLISLKAGGVGLNLTA
                     ADYVIHVDPWWNPAVEMQATDRTHRIGQDKPVFIYKLIVRDSVEEKILELQERKQRLV
                     TQLIATEKSFFKSLTPDDVAALFS"
BASE COUNT         2071 a         3048 c         3236 g         2049 t
ORIGIN      
        1 tcgcggagga tcacttgctg gcttgagtcg tagacaaaag accatgaaac agagtatcgc
       61 gcacattgct ttggtggtgg acgactatga cgaggccatc aaattttata cggagaaatt
      121 aaatttcact ctcctcgaag acaccgctca gagcgaaaca aagcgttggg ttttagtcgc
      181 gcctaagggg gcggaagaat gtagcttgtt gctggcgaaa ggggtcggcg atgaacaaag
      241 aagccgcatc ggaaaccaga cgggcgggcg agttttccta ttcttaaaaa ccgatgactt
      301 ctggagagat tataaaaaca tgctgtcgca agacatcgtt tttgtcagag aaccgaagaa
      361 cgaggactac ggaacggtcg ccgtcttcaa agatttatac ggcaacctct gggacctgat
      421 tgagttcaaa aaatagaagt gagtgaagtg tctcattgtc ctgattacca caccggaatg
      481 aatgagcgaa cgactaagcc aacgcgcgaa ggagacagcc ccccggccct ttcgatcatc
      541 atcccgacgc tcaacgaggc gcgctcaatc ggtcaaaccc tcgatgcggt catgggcgct
      601 cagggcgcgg tcgaggtgat cgtcgttgac ggcggaagcg ttgacgggac ggcggagatc
      661 gcgcgcgggc gcggggcgcg gctcgtcaca tcggggcgcg ggcgcggcgc gcagatgcac
      721 gcgggggcct gcgccgcgcg cggaagcgcg ctgtggttcc tccacgcgga cacgcgagcg
      781 tcagcaggtt gcgccgaagc cgtcgccggg gcattgcgtg accctgaggt ggtcgccggc
      841 aacttcagcg tgcgcttcga cggagacggt cgcgcggcgc gtttcctcac gtggctctac
      901 ccgcgtctgc gcctgctcgg tctctgctac ggcgactccg gtatcttcgt gcggcgcgaa
      961 gcttaccagc ggacgggcgg cttcaggccg tttccgatct tcgaggattt agatttggta
     1021 cgacggctgc ggaagacggg gcgcgtagtt tacctgcccg acatcgtcac cacttcctcg
     1081 cgccgttttg aagggcgaag cttcgccctc actttcgcca gatgggcgtt ccttcaggtg
     1141 ctctactggc tgggcgtgca tccgcgcaca ctcggccgga tgtacgctcc cgtccgcaac
     1201 gcgggtggga aatcgaaacg cgacgacgac gacccggcgg gcgcacgcgg cgtggcgtgt
     1261 tcagacgggc gcggacagta gtgagtcgtg ttccaacagc caacggtgtg tggcgggagc
     1321 acgttcgcgt gcttcctcgc tcgtgaagat ttcgtcacgc aagtgtagta agtcagagaa
     1381 agtgtccacg tcgtaccagt gcggaaggtc gagcacgcgc aggttcaggc gcgcggccgt
     1441 ttgggacgtt tgcctgtggg cgcggggcgt gctccaagcg acgttgcgga agaggccggc
     1501 gacgggttgg cgaagtccga cgaggtagta gccgccgtct tccgtagggc cgagagcgat
     1561 gtcggactcc ccggcggtga gcgaggcgat ggcgctctcg acgaacgagg tgggaagcgt
     1621 cgggctgtcg ctgcccgtga cgatgacggg gctgtggccg agcgtggcgg cgtgcgtgac
     1681 ggccgtcgag aggcgctcgc cgaggtcttc gccccgctgc tccagccaga agagattgcc
     1741 gggcggcagt agtgcttcga gcacgtcgcg cccgtcagcg ggggcgtaag ccacgatcac
     1801 ttcgggcacg acgcgccgca gggcggccac cgtgtcctgc gcgaagcagg ccgcgacgcc
     1861 agcggcctgc gcgcctgaga gcggcggcac gagccgcgtc ttgacctcgc ccgcgcgcgg
     1921 ggctttgacc atcacgatgc agacgggacg cataagagca aggatgaagg atgagggatg
     1981 agggatgaag aagagcattc aagccgaaac agagttactt tcatgcctca tccctcatcc
     2041 ttcatccctc atcctttgac ctgacggagt atcgcacagt ggcctattac gaagaagacg
     2101 atctgcaacg cttctcggag gtgggcaagc accgccccga cctgttcgag aaattcatgg
     2161 cgtggtatga ggcgtgtcag gaggacgggg cgctctcaag gcgcgagaag gcgctcatcg
     2221 gtctggcggt ggcgcacgcg ctccagtgcc cttactgcat cgacgcctac tctcaatcgt
     2281 gcctcgaagc cgggtcgaac atggagcaga tgaccgaggc gattcacatg gcttcggcgc
     2341 tgcggggcgg cgcaacactc atccacgctc ttcaggcgca caattcagtt aacaaggtct
     2401 cgatgtgatg tcgaaagccc tgccggtcat tcccgagggc ccaacctcgc gggcgcgtgc
     2461 aaccgtcagc ttcgacgaga agcttgcggc gcacggcaca cttctgcaac gcgccgaggg
     2521 gggcgtcgag accttgcagg tcaacgtcgg aaaactgtgc aatcaggcgt gcaagcactg
     2581 ccacgttgac gccggcccca aacgcaccga agtgatgaag cgcgaaacgg cggagcaggt
     2641 gattgccgcc gtggtgcgtt tccgtctgcc cacgctcgac atcacgggcg gcgcgccgga
     2701 gcttaaccca tcgtttcgct atctcgtcgc ggaggcgcga cgctctggcg cgcacgtcat
     2761 cgtgcgccac aacctcacgg tgatgttcga gccgggtcag gacgatctgc cggagttctt
     2821 ccgcgaacac gaggtcgaag tggtctcttc cttgccgtac ttcctcgaac agcagacgga
     2881 cgcgcagcgc ggcgcgggcg tgtttcagaa atccatcgag gcgctgcggc gtctgaacgc
     2941 agttggttat ggcggcgaag gaagcggccc agttctcaat ctcgtttata accccacggg
     3001 cgcgttcctg ccgcccgcgc aggagtcgat tgagagtgat ttcaagcgcg agctgtggac
     3061 gcgctaccgc gtcacgttcg accgcctcta tacgatcacg aacatgccca tcagacgctt
     3121 cctcgactac ctccgccgct ccggcaacga ggagcgttac atgcgtaagc tggtcgaggc
     3181 gttcaaccct tcgacggtcg aagggctgat gtgccgcaac ctcgtcagcg tagactggac
     3241 gggaaaactt tacgactgcg acttcaacca gatgctcgaa ctcggcgtcc ccgaaggact
     3301 gccccggaca atcgccgact tcgacccggc cgccttcgcc gggcggcgca tcgcgaccgg
     3361 cgcacattgc ttcggctgca cggccggctc cggctcatct tgcggcggcg cggtggccgc
     3421 atcttgaaat gacggaaaga accaacagcg cgaaacgggg cctcgtcttc gtctacaacg
     3481 ctgacagcgg tctgttcaat gccgtgagcg acgccgcgca taaaatcttt tcgccccgaa
     3541 cctaccagtg caatctttgc gcgctcaccc actcggccat tgggatgcgg agggagtgga
     3601 agcagtttct ggacggcctc aacgtgccgt tggagttcct gcacgccgac gagctgaaag
     3661 cgcgtgacgg cgtggcgggc gtgccgctgc cggccatttt taagcggggc gatgaggggg
     3721 tggaagtatt gatcgatgcg gagtcgatca acgcgtgtcg gacgagcgat gatttgaaac
     3781 agctaatcct taatcaactc tctgcgtgaa ctccgcgccc tctgtgttga gctggcttac
     3841 tacacactca actgcaagcc gcgatagtcg ctgaatctat ttcgccacgt acagctctgg
     3901 aagttgcttg cggagagctt ccaccttggg gaggtcgtgg atcacgatgt agggctgatc
     3961 cgggtgatgg acggcgtagt cctgatgata agcctcggct tcatggaaag atttgagcgc
     4021 ggcgacctga gtgacgattc gacgtttaaa gattttcgcc ccgttcagtt gaacgatgta
     4081 gctttcggcg atgcgctttt gttcctcgcc ggcgtaaaag atggccgagc ggtagtgcgt
     4141 gccggtgtcc ggcccctgcc gattcagttc cgtcggattg tgcgcgacgg aaaagaacac
     4201 cttgaggagc cggccatagg tgatctgaga aggatcgtaa gtgatgcgaa ccgattcggc
     4261 gtgcccggtg ttgcccccgg agactgattc atagtcagcc ttcttagcgt ctcctcccga
     4321 atacccggac accacgctcg tcaccccttt gacgtgttca aagacggctt cgacgcccca
     4381 gaagcagcct cccgcgagga cggccacctg ttcgcctttt acgtttgcca gcggcgcgtc
     4441 cacggccggg tcagggatgc cggcggaagg ggtcgtggtg gccacggccg cattgcaagc
     4501 gagggtcgcg ccgacgagaa acagagcaag aagtgcggaa agatgacgag tcatgttcaa
     4561 tatcctttca agggaacaca ccacttgacc gcattgtaag acgtacaagc gcggcgtgcg
     4621 aagtgttcga ctgcgaaatt agatgcgtcg ccgcacatgc cgggacgaac ttttgcagct
     4681 tttccatcct tcgattttca aatgtcggcg tgtgtcaggt cgtcttggga gttagaggtt
     4741 agaggtacga gtgtgaacga aacgaagtgt cacattgaac agggggcgac aattaaaaat
     4801 gacggttaga taacagatac accacagtta tcaatcaaca ttaagctgca caagttaagc
     4861 cataaaataa cccggttatt tttgatggaa ttttcaaggg gggagttatg tcttcaaata
     4921 cacgtccgct cgccatcgtc accggcgcat ccgccggcat cggctacgaa ctcgccaaac
     4981 tctgcgcaga taacgatgac ggctcacccg tcgccgccgc tcagcccggg atcgaagagg
     5041 cggcgcagac atttcgccag cacgagacat catctttact aactcagggg caatgattag
     5101 ttctgtcaag gggagaagca atgggataca tcacgacgcg cgacaacacg aagctctatg
     5161 tcaaagactg gggacggggc cgtcccgtca tccttctgca cggctggccg ctctcggcgg
     5221 atagctggga cgatcaggcc gtggcaatcg ccgacgccgg ctttcgcgcc attgcctacg
     5281 accggcgcgg cttcggtcgt tcggaacaac cgtgggaggg ttataactac gatactttaa
     5341 ccgacgatct cgcggccgtc gtcgaggaga cgggcgcaac tgacgtatct ctcgttggct
     5401 tctcgatggg cggcggcgag gtcgcccgct acatgtcgcg gcacggcggg aagaacgtgg
     5461 ctcaagccgt gctcgtctcg tcggttgtgc cgtacatgct tcagaccgcc gacaaccccg
     5521 acggtgttcc tcaggcgacg ttcgatcaga tgaccgcggg gatgaagcaa gaccgggcgc
     5581 atttcttcgc gggcttcttc aaagatttct acggcgtcgg actggtgtcg cagccggtga
     5641 gcgacgagct gctcgaaacg tcgcgcggcg tggcgatgca ggcgagcctg aaggcgacgc
     5701 tcgattgcgc caaagctttt gcgacgacgg atttccggcc cgacctggcg tctttctctg
     5761 taccgacgct aatcatccat ggcgccgacg ataagaccgt gcccatcgat gccaccgggc
     5821 gcgcatcggc gcgtggcatc ccgcacgcca agctcatcga gtacgacggc gcgccgcacg
     5881 gcctgttcgc cacgcacaag gagcgcctga cgagcgatct gctcgcgttc cttggtcagt
     5941 cagtgggtaa cgcggcccat ggtagccgtt aaaagggtag gcaacagttc agcacaagaa
     6001 agaggtaaat aatgaaagcg ctctgctggt acggtacagg tgatgtacgg gttgagaccg
     6061 tccctgatcc taaaatacta aatccgcgtg acgcgatagt gaagatcacc tcgaccgcaa
     6121 tctgcggctc cgacctccac ctcttcgacg gcttcatgcc gacgatggag gagggcgaca
     6181 tcctcggcca cgagccgatg ggcgtcgtcg ccgaggtcgg gaaagaggtc aaaaatctga
     6241 aggtgggcga ccgcgtggtc gtcccgttta cgatttcctg cggcgaatgt ttcttctgcc
     6301 agaaggagat gtactcgctc tgcgacaact cgaacccgaa cgcgaaaatc gcgcgcgcgg
     6361 cgatgggtca ttcgccggcg gggctgttcg gcttctcgca tatgctcggc ggctacgcgg
     6421 gcggacaggc tgaatacctg cgcgtgccgt tcgccgatgt cggcccgctc aagatcgaga
     6481 acgactcgct cacggacgag caggtcttgt ttctctccga cattttcccg accggctaca
     6541 tggcggcgga gaactgcggc atcgagaaag gggacacggt cgccgtgtgg ggttgcggcc
     6601 ccgtcgggca gatgaccatt cagagcgcgt ggatgctcgg agccgagcgg gtgattgcca
     6661 ttgacagggt gccggagcgg ctggcgatgg cggcgaagca cggcaaagcc gagaccatca
     6721 actttcagga cgagaaagtt tatgaccagt tgattgagat gaccaaaggg cgcgggcctg
     6781 atcgctgcgt cgatgcggtc ggggccgaag cccacgctac cggcagcatt gactctgtgc
     6841 tcgacgcggc gaagacggcc gtgtatctcg gcaccgaccg cccgcacgtc atccgcgagg
     6901 cgatcatgtg ttgccgcaag ggcggcacgg tctcgattcc cggcgcttac gtcggggtca
     6961 tggacaagct gccgctcggc gctctgatga ataaagggtt gacgatcaag acggggcaga
     7021 cgcacgtgca gcattacaat aaaccgctgc tcgaaaagat cgaggcgggg gaaatcgacc
     7081 ccagcttcgt catcacgcac cgcgtcaagc tcgacgatgc gccgaaggct tacgcgactt
     7141 tccgcgacaa ggaagacggc tgcatcaagg tggtattgaa gccataaaac tccgcagccg
     7201 tccgtgtcgg ctcacgcatg ctctgcaagt tccgcccagc ggtcaaggtc gcgggctaat
     7261 tgctctgtca actcctcacg ctcctgatag agctgatgca cgaggtcggc gttgcttgag
     7321 ttcaaggtca attcggtttc tatctcgacc tcacgacgtt cggcggcctg aatgcgtgct
     7381 tccgtggcct ctaactcttt ccgctctttg aatgagagtt tgcgattggc ggaaggtgga
     7441 gccacgcgct gcctaaccgg cggccgcacg gctgttgctt ccacggtagg agcgccgtcg
     7501 cgctcctgct ttcgatgcgc ttcgagaaac gcggagtaat tgccggggta ttctcgcagt
     7561 cgcccttcac cctcgaagcg gaagatgtgc tcaaccgtgc ggtcgagaaa atatcggtcg
     7621 tggctgacga caacaaggtt gccgccgaag ccgtccagat agtcttcgag cgtgaccaga
     7681 gtttgaatgt cgagatcgtt cgtcggctca tccagcagca ggacgttggg cgcgcccatc
     7741 agcacgcgca gaaggtaaag ccgccggcgc tcgccgcccg aaatttttcc tataggcgca
     7801 tactgcatcg cgcctgtgaa caggaacttt tcgagcatct gcccggcggt gataaatgtt
     7861 ccgtccgcgg tctcgatccg ttcggcgacc gcgcggatgt aatctatcac ccgctcctgc
     7921 tcgtcgaggg cgcggctctc ctgatcgtag taaccgaagc gaaccgtctg gcccacttcc
     7981 acaaagcccg tgtccggtgc gactctaccg gcgatgatct caagcagcgt ggtcttgccc
     8041 gcaccgttcg ggccgatgac gccgatgcgc tcgccgggct tgagcgtgcg gctaaaatct
     8101 tcaatcaaca cccgaccgcc atacgacttc gtaatgttgc ttagctccaa cactttctta
     8161 cccaaacgct ttgagccgac cgacatgtcg agcacagctc tggccgcctc tttgggttgc
     8221 gcgatcaggc tctcggcgcg gtctacacgg gccttcgatt tgcgcgtgcg cgccttcgcg
     8281 ccccgccgca gccacgccag ctcgcggcgg atcaacatct cgcgtttctg cccctcggcg
     8341 gctcgcgcac tctcctgctc ctctttcttt tcaaggtagt aggcgtaatt cccgtcgaac
     8401 gcctgcaccg cacttcgatc aatttcgagc attcgcgtgg ccacacggtc gaggaaataa
     8461 cggtcgtgtg tgactaagag gagcgcgccc gtgtagcgtt cgagataacc ttcgagccag
     8521 gagatcgtct cggcgtcgag gtggttggtc ggctcgtcga ggatgaggag actcggacgt
     8581 tcgatcagtg catgcgcgag cgcgacgcgc tttcgctgtc cgccggagag cgcgcccatt
     8641 ctggcgttgg tatcggtgat gcccagcttg ctcagaacca tccgcgcgtt tgtctccatt
     8701 tcccacgcgc ccgacgcctc caaacggtgc gccagttccg acacgcgatt gagcgcgcgt
     8761 tgttcatccc cgcttttagt cgaaagctcg cggcaagcgc gctcgtagtc gagcagcagt
     8821 ttcatcttgg catcgcttgc ggcgaagacg gcgtccagca cagtctcgtc cgcaacgtaa
     8881 ggcggattct gaggcagata tccgatggaa acgccctcgg cgagagtgat gcgtccggcg
     8941 tccggccttt cttcaccggc gatgaggcgg agaaaagttg tcttgcctga gccattgaca
     9001 ccgacgacgc caacgcggtc gtcgtcattc aagccgagac tgacgttctc gaaaagaggc
     9061 cgtaggccat agtctttgct gactgcttcg acggatagaa tatgcattgt cataaaaagg
     9121 gcgagccgtc gtcgcggatc acaagcgacg ccgggttcga tagtccaatc ttaatttcgc
     9181 catgaagctt actacgaaaa agcccccgac ttggaggctt tcgactactg aacggtatcc
     9241 aagttgagcg tttgtcagaa cgcaaactcg gccctataaa tgaagccaaa aggcggacac
     9301 gctaatccac gcggtaataa tagagggtca agagaacaac gccgccacgt cgtcgggggt
     9361 gagcgacttg aaaaagcttt tctcggtggc gatcaactgc gtgacgagtc gctgcttgcg
     9421 ttcctgcaac tcaagaatct tctcctcgac cgagtcgcgc acgatgagct tgtagatgaa
     9481 gaccggctta tcttgcccga tgcggtgcgt gcggtcggtg gcctgcatct cgaccgcggg
     9541 gttccaccac ggatcgacgt ggatcacgta gtcagccgcc gtcagattca agccgacgcc
     9601 gcccgccttg aggctgatga ggaagaaggg gattgacggg acgctctgaa aagcgtccac
     9661 gcgctcctgc cggtcgcgcg tctgaccgtc gagataagag aagggaatgc ggcgcttctt
     9721 caattcgccc tcaaccagct tcaacatctg cacaaactgc gagaagatca gcgccttgtg
     9781 tccttcggcg tgtagggtcg agagagtctc cattaacagt tcgagcttcg ccgagccgcc
     9841 cctgtatgtc ttcaccgcaa ggcgcgggtg attgcaaatc tgccgcaggc gcagcaagcc
     9901 ttcgagcacg cggaaacgcg cgttgttgat gccctcgtct tcgatcagct tgagcaaaag
     9961 cgcgcggtag tagtcgcgcc gctcgtcgta gagcttgcgc tgcgcgggct ccatgtcgca
    10021 gaccatctgg cgctccgtgc gcgggggcaa atcctttgcg acttggtctt tggtgcgccg
    10081 caggatgaag gggtaaatca ttttgcgcag gaactgcgcc gcctcctcgt cgcccttgcg
    10141 ctcgatgggc gtggcaaact cttcgcggaa atattcggcg ttgcccaaaa gtcccggatt
    10201 caaaaaggcg aactgcgacc agagttccag cgtgttgttt tccacgggcg tgcccgtcag
    10261 ggtcaggcga tgtgccccgc gcaggaggcg cacggctttg gccgtctccg cgagcggatt
    10321 cttgatcgcc tgcgcttcgt cgagaatcag gtagttgaac tcgtaatgca tcagcatctc
    10381 tacgtcgcgc cggagcagcc cgta
//
LOCUS       DASVES010000026         8784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_362697, whole genome shotgun sequence.
ACCESSION   DASVES010000026 DASVES010000000
VERSION     DASVES010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362697"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VF240_01060"
     CDS             complement(<1..610)
                     /locus_tag="VF240_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7173859.1"
                     /translation="MSPDFRQRSNELERLDTGDYTSEEYEDCLVELRRVNRWLGDAHA
                     LRRSVLAAIGRDGLTEFSLLDVGAGSGELLRSAALWARGEKRGARLVGLELNARAARA
                     VLEESVEFKEIRAVRADALRMPFADGAFDYVMCSLFTHHFRDEALVALLREMSRVARR
                     RVFVIDLHRHRVAHLFYTTIARLFLRSPLVRADGALSILRSFV"
     gene            complement(658..1923)
                     /locus_tag="VF240_01065"
     CDS             complement(658..1923)
                     /locus_tag="VF240_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7173860.1"
                     /translation="MLGRLKQPRRVVITGVGCVTPLGVGREAFWGALCRGESGVRRIE
                     GFDVTSSPVQIAAEVRGFDWEQELKPKDRRHVARTVPLALAAARGALDDARLQPSDFS
                     TEERRAFGVVLGTGGGGLAFTEAQYKYFYTDAPGTASIYTIPASTHGGLSSELSMAFG
                     LRGLSHVISTGCTSSTDAIAYAAQHIAVGRQDAMLAGGSDAPLAPGILAGFVLMKVLT
                     EDWNAEPARASRPFSRDRSGMVIGEGAWVYVLEELESARRRGARIYAEVTGYGATCDA
                     YHRVRLEDTGEEPARAMRLALEDAGREAREVDYVNLHGTSTLLNDRIETQALKLALGD
                     EAKRIPMSATKSQIGHPQGASGAAGVAAALCAMTESVIPPTINLDEPDPACDLDYVPN
                     RAREGRVRVALCNCIGFGSKNSALVLEKI"
     gene            complement(2024..2524)
                     /locus_tag="VF240_01070"
     CDS             complement(2024..2524)
                     /locus_tag="VF240_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX7173861.1"
                     /translation="MGTRSGETTRAFGGEQSWRGRLWRYGPLVAWIGLIFFASTGNLS
                     GENTSLVVRPLLLWLFPDISEPELLRAHLFIRKAAHFTEYAFLALLAARAFLTSSRPW
                     LKRRWAVVALLLVAACALLDEYHQSFVPTRGGSVYDSLIDMSGGALALAFVSLWRSRR
                     RRREGP"
     gene            complement(2702..3601)
                     /gene="atpG"
                     /locus_tag="VF240_01075"
     CDS             complement(2702..3601)
                     /gene="atpG"
                     /locus_tag="VF240_01075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX7173862.1"
                     /translation="MPSLLDIRRRIRSVKNTQQITKAMKMVSAAKLKRAQDRVVSARP
                     FANKMTEVLSELASHTEEDFHHPLLDARGDERYLLVLITADKGLAGAFNANLIKAAQA
                     FVREHADKAVELYAVGRKGRDFFRRRAFTIAAENIGLTGKGRVEYGEAVEVARDITRR
                     FIEDEGIDKVFLVYSEFKSVLSQRAVVEQILPVSRVVERAEDEASSADPSRPVTFVDY
                     IYEQPPEDIFSQLLPRLIETQIYRALLESVASEQGARMTAMDSASKNAFDLIAKLTLN
                     MNRVRQAGITREIIEIVSGASVQ"
     gene            complement(3741..5339)
                     /gene="atpA"
                     /locus_tag="VF240_01080"
     CDS             complement(3741..5339)
                     /gene="atpA"
                     /locus_tag="VF240_01080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7173863.1"
                     /translation="MEPIRANEISDIIRQQIENFEAGVTVTEVGTVIKVGDGIAEIHG
                     LEKVMAGELIEFPHDVRGLALNLEEDKVGAVLFGEFQQIKEGDTVRRTGRIMSVPVGD
                     ALIGRVVDALGRPVDDRGPIITEDFNPLERIAPGVVDRQPVKEPLQTGLKAIDSMVPI
                     GRGQRELIIGDRQTGKTAVALDAIINQRGKDVICIYVAIGQKASTVAQVIKTLTDFGA
                     MDYSIVVNASASDPAAMQYLAPYAGAAIGEYFRDTGRHALTIYDDLSKHAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSEAKGAGSLTSLPLIETQAGDISAYIPTN
                     VISITDGQIFLESDLFNSGIRPAINVGNSVSRVGGSAQIKAMKQVAGTLRIDLAQFRE
                     LQAFAQFGSDLDKATQAQLARGQRLVEILKQPQYQPMEVEKQVLLIWAATNGHLDEVP
                     VDQVRRFESEMLRFVENAHPGVLANIREKKSFTDEIKAELKQLVSDFKGSWEDKAFNP
                     QPDPPRLAETAAATPAAAPAVAGD"
     gene            complement(5438..5986)
                     /gene="atpH"
                     /locus_tag="VF240_01085"
     CDS             complement(5438..5986)
                     /gene="atpH"
                     /locus_tag="VF240_01085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX7173864.1"
                     /translation="MSIPVAVARRYSAALADVITARGEGREVQEELSAWEEMGRANGL
                     LQEVFRNPTIPYEQKHRVVRALIERTRVRPTTANLLLVLLQNQRLADLSEINKALAQE
                     LDRRSGIISAQVTTARAVPQQTQEVLRTRLAEMTRSKVRLEFAVDDDLIGGVVTRIGS
                     TVYDGSVRNQLQQIKQRIVGEQ"
     gene            complement(5983..6516)
                     /locus_tag="VF240_01090"
     CDS             complement(5983..6516)
                     /locus_tag="VF240_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX7173865.1"
                     /translation="MVTACMIFGFADTSGGGIVSLETVAKLINLLIFVALAVYFLRRP
                     LSEKLRERREGIRRALMRAQEERDAALAKLAEVEERLGRLDAEVEAVRVHARREAEEE
                     RERIKRQTEEDAIKIRSQAQREIESVSKAARAGLRTFAAEQSVRLAEELIRHEMRPED
                     DARLVSEYVEDLGGVRS"
     gene            complement(6559..7020)
                     /locus_tag="VF240_01095"
     CDS             complement(6559..7020)
                     /locus_tag="VF240_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX7173866.1"
                     /translation="MTVSLVLAESIQLVPDGTIFLHIAIIIAMVFVLNRILFKPINKI
                     LNDRERLTTGNSTEAQVILRRVEDNLSTYERSLKEARAESYRLLEDERTKAINERQSL
                     MRTVRDEVDQIVDNEEKLIESQAAEARITLEEEARRMAVTIKNQILGRQVG"
     gene            complement(7242..8051)
                     /locus_tag="VF240_01100"
     CDS             complement(7242..8051)
                     /locus_tag="VF240_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7173867.1"
                     /translation="MANEELSEIEIDLIEPNSLQPRTNFDNEQLENLAQSIRANGIIQ
                     PLLVRRIDEGRYQLIAGERRWRAAQKAELQRVPAIIRDIPDDKILELALIENIQRQEL
                     NPIEEAQAYKKLIDTLGLTQEMVAQRVGRDRTFITNYIRLLRLPEDIQRLVEQEKISM
                     GHARALLGVDDIEIQRKIAQNVIDQSLSVRETERAIRKIIEGASAVAATARTQPQNDA
                     NIRAAESKLRRKFSSQVRIINNQNGEGGKIEIEFYSENDLQRVYQLLVGTQ"
     gene            complement(8041..>8784)
                     /locus_tag="VF240_01105"
     CDS             complement(8041..>8784)
                     /locus_tag="VF240_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7173868.1"
                     /translation="LATSELRVLLVDADPQANATSGVGIQRGSLRRSLYHILVLDERV
                     SNVIQPTELPFLKILPADKNLIGAEVELVDQERREYKLKTALQQVIDEFDFIIIDCPP
                     SLGLLTLNALTSANSLLVPIQCEYYALEGVTELFDTLARIRRSINPTLSIEGLLLTMY
                     DERTNLSTAVAADLRDFYGSQVFETIIPRNVRLAEAPSYGKPIIIYDIRSRGAESYLQ
                     LAKEIMSYDTKGSGSRLERAATGYSGDGQ"
BASE COUNT         1666 a         2688 c         2569 g         1861 t
ORIGIN      
        1 ggacgaagct tcgcaggatg gagagcgcgc cgtccgcgcg cacgagcggg ctgcggagga
       61 agaggcgggc gattgtcgtg tagaagaggt gtgcgacgcg gtgcctgtgc agatcgatga
      121 cgaagacccg gcggcgcgcc acgcgcgaca tctcgcgcag gagcgcgacc aacgcctcgt
      181 cgcgaaagtg gtgtgtgaag agcgagcaca tcacgtagtc gaacgcgccg tcggcgaatg
      241 gcatccgcaa agcgtcggcg cgcacggcgc gaatctcttt gaactccacg gactcttcaa
      301 ggacggcgcg ggccgcgcgc gcgttcaact caaggccgac gagccgcgcc ccacgtttct
      361 ctccccttgc ccaaagggcc gccgagcgca gcaattcgcc cgagccggcg cccacgtcga
      421 gcaacgaaaa ctccgtcagg ccgtcgcggc cgatggcggc gagtacggag cgacggagcg
      481 cgtgcgcgtc gccgagccag cggttgacgc ggcgcagctc gacgagacag tcctcatact
      541 cctcgctcgt atagtcgccc gtgtcgagtc gctcaagctc gttgcttcgc tggcggaagt
      601 caggagacat gtcgaacggc ggcccgcgtc cttaacgccg gaaatcgaac acgcatctca
      661 aatcttttcc aatacgagcg cgctgttctt cgagccgaag ccgatgcagt tgcagagcgc
      721 gacgcgcacg cgcccctcgc gtgcgcggtt gggcacgtag tcgaggtcgc acgcggggtc
      781 tggctcatcg aggttgatcg tcggcgggat gacactctcc gtcatcgcgc agagtgcggc
      841 ggcgacgccg gccgcgcccg acgcgccctg cggatgtccg atctgcgact tggtggccga
      901 catcggaatc ctcttcgcct cgtcgccgag cgcgagcttg agcgcctgcg tctcgatgcg
      961 gtcgttgagc agcgtcgagg tgccgtggag gttgacgtaa tcgacctccc gcgcttcgcg
     1021 gcccgcgtct tcgagcgcga gtcgcatggc gcgcgccggc tcctccccgg tgtcttcgag
     1081 gcggacgcgg tggtaggcgt cgcacgtcgc gccgtagccc gtgacctcgg catagatgcg
     1141 cgcgccgcgc cgacgcgcgc tctccaactc ttcgagcacg tagacccacg cgccctcgcc
     1201 gatgaccatc cccgaacggt cgcgcgagaa gggccgcgag gcgcgcgccg gctccgcgtt
     1261 ccagtcctca gtgagaactt tcatcaggac gaagccggcg aggatgccgg gcgcgagggg
     1321 cgcgtcagag ccgccggcga gcatcgcgtc ttggcggccg acggcgatgt gctgagcggc
     1381 gtaggcgatg gcgtccgtcg agctggtgca gcccgtcgag atgacgtgcg agaggccgcg
     1441 caggccgaag gccatggaga gttcggagga cagtccgccg tgggtcgagg cggggatggt
     1501 gtagatgctc gcggtgccgg gcgcgtccgt gtagaaatat ttgtactgcg cttcggtgaa
     1561 ggcgaggccg ccgccgcccg tgccgagcac gacgccgaag gcgcggcgct cctcggtcga
     1621 gaagtcggac ggctggaggc gtgcgtcgtc gagcgccccg cgcgcggccg ccagtgccag
     1681 cgggacggtg cgcgcgacgt gtcgtcggtc tttcggcttc aactcctgct cccagtcgaa
     1741 gccgcgcacc tcggcggcga tctgcacggg tgaggaagta acgtcgaagc cctcgatgcg
     1801 ccgcacgcca ctctcccccc gacacagcgc gccccagaac gcctcgcgcc ccacacccag
     1861 aggcgtcaca caccccacgc ccgtgatgac gacgcggcgc ggctgcttca aacgtccgag
     1921 catgattgga atacgctttc cgacgggctg ccagattggt tcgcagattt ttgggagact
     1981 cggtcaatca taacacgccc cgcgggggcc ggcgtcgccc gcgctacggc ccttccctcc
     2041 gtcgccggcg cgagcgccac agcgacacga acgcgagtgc taaggcgccg ccgctcatgt
     2101 cgatcaggct gtcgtagacg gagccgccgc gcgtcggcac gaagctctga tggtactcgt
     2161 cgagcagcgc gcaggcggcg acgagcagga gcgcgacgac ggcccagcgc ctcttcaacc
     2221 acggacgcga cgacgtgagg aacgcgcgcg cggcgagcaa cgcgaggaag gcgtattccg
     2281 tgaagtgtgc ggccttgcgt atgaacaggt gtgcgcgcag gagttccggc tcgctgatgt
     2341 cggggaagag ccagagcagg agcgggcgga cgacgagcga agtgttctcg cccgacagat
     2401 tacccgtgga ggcgaagaag atgaggccga tccacgcgac cagcggcccg tagcgccaga
     2461 gccgcccgcg ccagctctgc tcaccgccga acgcccgtgt cgtctctccg cttcgcgtgc
     2521 ccatgttctt catacaaatt tcgggtcgtc tatcaaccct ccgcgtgaac tctgcgtcct
     2581 ctgcgcctcg gcggtgaatc tactttgccc tcactcaagt gggatgcaac cctcgacaaa
     2641 gcaaaagagg gaagccggcc gcgccgcttc cctcacccga aacttttctt caccttctcc
     2701 gctattgcac agaagcgccc gagacgatct cgatgatctc gcgcgtgatg cccgcctgcc
     2761 gcacgcggtt catgttgagc gtcagcttgg cgatcaggtc aaaggcgttc ttcgacgccg
     2821 agtccatcgc cgtcatgcgc gctccttgct ccgaggcgac cgactccaac agcgcgcggt
     2881 aaatctgcgt ctcgatgagg cgcgggagga gttgactgaa gatgtcttcg gggggctgct
     2941 cgtagatgta atcgacgaac gtcacggggc ggctcgggtc ggccgacgac gcctcgtcct
     3001 ccgcccgctc cacgacgcgc gagacgggaa gaatctgctc gacgacggcg cgctgcgaga
     3061 gcaccgactt gaattcgctg tagacgagga agaccttgtc gattccctca tcctcgatga
     3121 agcgccgcgt gatgtcgcgc gcgacctcga cggcctcgcc gtactcgacg cgccccttgc
     3181 cggtgagccc gatgttctcg gccgcgatgg tgaaggcgcg gcggcggaag aagtcgcgcc
     3241 ccttgcggcc gaccgcgtag agttcgacgg ctttgtcggc gtgttcgcgg acgaaggcct
     3301 gcgcggcctt gatgaggttg gcgttgaagg cgccggccag acccttgtcg gccgtaatca
     3361 gcacgagcag gtagcgttcg tcgccgcgcg cgtcaagaag ggggtggtga aaatcttctt
     3421 cggtgtggga ggcaagctcg ctcaacacct ccgtcatctt gttcgcaaac gggcgcgccg
     3481 agacgacgcg atcctgcgcg cgcttgagct tcgccgccga gaccatcttc atggccttcg
     3541 taatctgctg cgtgttcttg accgacctga tgcgccggcg gatgtcgaga agacttggca
     3601 taaaaactgt tgtcagtagt tagttgtcag taatcagttg ctctcgtcac ccggcttgag
     3661 ccttcagacg tgagcggcaa gttgcccgac aggaaacaac tgacaactga ccagggacta
     3721 ctgaccgaat tcgttgtgcg ttagtcgccg gcgacggcgg gcgcggcggc cggcgtcgct
     3781 gccgctgtct ccgccagccg cggcgggtcg ggctggggat tgaacgcctt atcctcccag
     3841 ctccctttga agtcgctgac gagctgcttg agttcagcct tgatttcgtc agtgaacgac
     3901 ttcttctcac ggatgttcgc cagcacgccg gggtgcgcgt tctcgacgaa gcgcaacatt
     3961 tccgactcga agcggcgcac ctgatcgacg ggtacttcgt cgaggtggcc gttcgtcgcg
     4021 gcccagatga ggagcacctg cttctccact tccatcggct gatactgcgg ctgcttgagt
     4081 atctcaacga ggcgctgccc gcgcgcgagc tgcgcctgcg tggccttgtc gagatcggag
     4141 ccaaactgtg cgaacgcctg tagctcgcgg aactgcgcga ggtcgatgcg cagcgtgccg
     4201 gccacctgct tcatcgcctt gatctgcgcc gaaccgccga cgcgcgacac tgagttgccg
     4261 acgttgatgg ccgggcgaat gccggagttg aaaaggtcag actccaaaaa gatttgcccg
     4321 tccgtgatcg agatgacgtt cgtcggaatg tacgccgaga tgtcgccggc ctgtgtctcg
     4381 atgagcggca gggacgtgag cgagccggcg cccttcgcct ccgacaactt cgcggcgcgc
     4441 tcaagcaatc gcgagtgcag gtagaacacg tcgccggggt aagcctcgcg tccgggcggg
     4501 cggcgcagca gcagcgaaat ctcgcggtag gcggcggcgt gcttcgagag gtcgtcgtag
     4561 atggtgagcg cgtggcggcc ggtgtcgcgg aagtattcgc cgatggccgc gccggcgtag
     4621 ggcgcgaggt actgcatcgc cgccgggtca gacgccgagg cgttgacgac gatggagtag
     4681 tccatcgcgc cgaagtccgt cagcgtcttg atgacctgcg ccacggtcga agccttctgt
     4741 ccgatggcga cgtagatgca gatgacatcc ttgccgcgct ggttgatgat cgcgtcgagc
     4801 gcgacggccg ttttgcccgt ctgccggtcg ccaatgatca gctcgcgctg gccgcgcccg
     4861 atgggaacca tcgagtcgat ggccttcagg cccgtctgca aaggctcctt cacgggctgc
     4921 cggtcaacga cgcccggcgc gatgcgctcc agcgggttga agtcctcggt gatgatgggg
     4981 ccgcggtcgt caactgggcg gccgagcgcg tcaaccacgc gcccgatcaa cgcgtcgcct
     5041 acggggacgc tcatgatgcg cccggtgcgc ctgacagtat cgccctcctt aatctgctga
     5101 aactcgccga agagcaccgc gccgactttg tcctcttcca ggttgagcgc aagcccccgc
     5161 acgtcgtgtg ggaactcaat caactcgccg gccatcacct tttcgagacc gtgaatctcg
     5221 gcgatgccgt cgcccacctt gatgaccgtg cccacctccg tcacggtgac acccgcctcg
     5281 aagttctcga tctgctggcg aatgatgtcg ctaatctcgt tggctctgat cggttccata
     5341 aactttaact cgcctgttct gaattactcc gaaaatgtcg ggcctcaaac ttctcgctgg
     5401 gcgccgatga gtcgggcatc ggtcgtgggc aactaaatta ttgctcgccc acgatcctct
     5461 gcttaatctg ctgcaactgg ttgcgaaccg agccgtcata gacggtggag ccgatgcgcg
     5521 tcacgacccc gccgattaaa tcatcatcga cggcgaactc aagacgcacc ttacttcgtg
     5581 tcatctccgc cagacgcgtc cgcaacacct cttgcgtctg ttgcggcacg gcacgggccg
     5641 tcgtgacctg cgccgagatg attcctgagc gccggtcaag ctcctgagcg agcgctttgt
     5701 ttatctcact taagtctgca agccgttgat tctgaaggag tacgaggagg agattggccg
     5761 tcgtcggacg cactcgcgtg cgctcgatga gggcgcgaac cacacggtgc ttttgctcgt
     5821 agggtatcgt cgggttgcgg aagacttctt gcagcaggcc gtttgcgcgc cccatctcct
     5881 cccaagccga caactcttcc tgaacttctc gcccctcacc tcgtgccgtg attacgtctg
     5941 ccagcgcggc cgagtatcgg cgcgccaccg ccaccggtat gctcaagacc tgacccctcc
     6001 taaatcttcg acgtactcac ttacgaggcg cgcgtcgtcc tcgggccgca tctcatgtcg
     6061 aatcaactcc tcggccaaac gaacgctctg ctccgcggcg aaggtccgca atcctgcccg
     6121 cgcggcctta gaaacacttt cgatttcgcg ttgcgcctga ctgcgtatct tgatcgcatc
     6181 ctcttccgtc tgtcgcttga tgcgctcgcg ttcctcttcg gcctctctgc gcgcgtgcac
     6241 tctcactgct tcaacctccg catcgaggcg ccccaaccgc tcctccacct ccgcgagttt
     6301 tgccagtgcc gcgtcgcgct cttcctgtgc gcgcatcaag gcccggcgta ttccctcgcg
     6361 gcgctcacgc agtttctcac tcagcgggcg gcgcaggaag taaacagcca gggctacaaa
     6421 gatgagtagg ttgatgagtt tcgcaaccgt ttcgagcgaa actatccccc caccgcttgt
     6481 gtcggcaaat ccgaagatca tgcaggccgt taccatctaa cttggtttat gcaaaaaatg
     6541 ttcttgaaat gagggcattc agccgacttg gcgacccaaa atctggttct ttattgttac
     6601 agccatgcgt ctggcttctt cttccaacgt aatgcgcgcc tcagccgcct gcgactcaat
     6661 taacttctct tcattgtcta caatttgatc gacttcatcg cgcacagtac gcatcaggct
     6721 ttgcctctca ttaattgcct ttgtgcgctc gtcttcgagc aggcgataac tttccgcgcg
     6781 tgcctctttc agagatcgtt cataggttga aagattatct tcgacacgcc gtaaaataac
     6841 ctgcgcctcg gttgaattac ccgttgtgag acgctctcta tcattcaata ttttattaat
     6901 cggcttgaat aaaatcctgt ttagaacaaa gaccatcgcg ataattatcg cgatgtgaag
     6961 gaagattgtg ccatccggca caagttgaat cgattcggca agtactaatg aaaccgtcat
     7021 gagagcctta ccagtaagtc atgtcaccac tatcggtggc aatagacatt aagatgttgt
     7081 gtgttaaaag ggttggagtt taacacgcca tttcgagacg cgtcaatgag ggtgcagaat
     7141 tgctaattga acaccgcctg caaaacttca aatttttact ctttaacatc gcagataaat
     7201 acggcaaagt cgctcattac aggctcttta cctatgacag gctattgcgt ccctaccaac
     7261 agttgataaa cgcgctgtaa gtcattttca ctataaaatt cgatttctat cttccctccc
     7321 tcgccgtttt gattattgat aattctaacc tgtgaactaa attttcgacg gagcttagac
     7381 tccgccgccc gaatgttggc gtcgttttga ggttgcgtcc ttgcggttgc ggcgaccgca
     7441 ctggcgcctt caattatttt tcttatggcc cgttcggttt ctctaactga gagagattga
     7501 tctattacgt tctgcgctat ttttctctgg atttctatgt cgtcaacccc gagtaaggcc
     7561 cttgcgtgcc ccatcgaaat tttttcctgc tctaccagcc tttgtatatc ttcaggcaga
     7621 cgcaagagtc ttatgtagtt ggtaataaaa gtgcggtctc taccaaccct ctgggcgacc
     7681 atttcctgag tcaaacccag ggtatcgatc aacttcttgt aggcctgagc ttcttcaatc
     7741 gggtttagtt cctgacgttg aatgttttca attaacgcca gttctaatat tttatcgtcc
     7801 ggtatgtcac gtatgattgc cgggactctt tgcagttctg ccttctgtgc agcccgccac
     7861 cttctctcac ccgcgattaa ttgatagcgt ccttcatcta tgcgtcttac taagagcggc
     7921 tggattatcc cattggcgcg aatggattga gctaagtttt ctaactgctc attgtcaaaa
     7981 ttagttctcg gctgaagtga attgggttct atgagatcga tttcaatttc agataactcc
     8041 tcattggcca tcacctgagt atccggtagc agcgcgctca agcctcgacc cagagccctt
     8101 cgtgtcataa gacattatct cctttgcaag ttgaagatag ctttcagccc cacgagaacg
     8161 tatgtcatag ataataatag gcttaccgta gcttggcgcc tccgctagac gaacatttct
     8221 gggaattatg gtttcaaaaa cctggctccc ataaaaatcc ctcaaatcag cagcgaccgc
     8281 cgtcgataga ttcgtccttt catcatacat tgtcagtagg agaccctcta ttgacaaggt
     8341 aggattgatt gaacgccgaa ttcgggccag tgtatcaaac aactcagtca ccccttcgag
     8401 tgcataatac tcgcactgaa tcggcacaag tagtgaattt gcgctagtta gggcgttgag
     8461 tgttaagagt ccgagagagg gaggacaatc aattataata aaatcgaatt cgtctataac
     8521 ctgttgcagc gcagtcttga gtttgtactc tcgccgctct tgatcaacta gttcaacttc
     8581 tgccccaatg aggtttttgt ctgccggcag aatcttcaaa aaaggcagtt cggtgggttg
     8641 aattacattc gagacccgtt catcaaggac gagtatatga tacagactcc ggcgaagact
     8701 ccctctttga atgcccacgc ccgaagtcgc attcgcctgc gggtctgcat caaccagaag
     8761 gacacgcaac tcagaagtgg caag
//
LOCUS       DASVES010000027         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_163984, whole genome shotgun sequence.
ACCESSION   DASVES010000027 DASVES010000000
VERSION     DASVES010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163984"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2175
                     /locus_tag="VF240_01110"
     CDS             <1..2175
                     /locus_tag="VF240_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7173869.1"
                     /translation="VDLRLDARRMLKVPWTAHLGLHDDETSELCHWHIPEAHFLETWS
                     DARAFDGTASIIQPLIAPLYNGKSVHEFLVAFTARPDRSGYDIVRDFWRTQISGGSSA
                     PLLAGARLLAATAGRQAPASQTPVPAVGPQPSPGTSAAQPQPTQGAQTQPTPSASTTP
                     GGGSPATRSSGTGAGAALSAAAADDPFERAWRKALHDGVVPGTAAREQGRTANANFIN
                     ETAPGGTGAGASPTAAGASRATPEVVVVAPPSSGGAGEYEIVFRPDPTIYDGRFANNG
                     WLQELPKPLTKLTWDNAALVSPNTAERLGLGTTPDGAQLAPARRVNRMTAKGGEIVGD
                     LIRIGFKGQSLTVPALIVPGQPDGVITLHLGHGRWRVGRVGGRDGTFEQPTGTGFNVY
                     KIRHSDALWHGRGVEVSKTEETYPLATTQIHFQMEGRDVVRSGSLEQWREHPEMAPET
                     EHSVPVQPAGKNREPARPESMYPDYDYSQRDEQGERGYRWGMVIDLGACVGCNACVIA
                     CQAENNIPVVGKEQVLRSREMHWLRVDAYFRGAVENPTGVHFMPVPCMHCENAPCEPV
                     CPVHATVHSAEGLNDMVYNRCVGTRYCSNNCPYKVRRFNFLLYQDWETPSLKMLRNPE
                     VTVRSRGVMEKCTYCVQRIEYAKIEAEKEGPRRVRDGEIVTACQAVCPSEAIVFGDLN
                     DPEARVTKMAHEPRNYSLLADLNTRPRTTYLAAVRNPNPNLG"
     gene            2325..>3032
                     /gene="nrfD"
                     /locus_tag="VF240_01115"
     CDS             2325..>3032
                     /gene="nrfD"
                     /locus_tag="VF240_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7173870.1"
                     /translation="MSQIDNPHDDTRIHTTPAVIEPGHTFGSVTDKISAIVLRKRTPL
                     GWYIGFGISFMITGVLMGTILWLVLNGIGIWGNNQPVGWGFDIINFVWWIGIGHAGTL
                     ISAILLLLRQSWRTSINRFAEAMTLFAVASAGLFPLLHTGRPWLAYWMLPYPNTMGMW
                     PQFRSPLMWDVFAVSTYATVSALFWYTGLIPDLATLRDRARSKFGRVVYGMAALGWRG
                     SARHWARYEMTYLLLAGL"
BASE COUNT          521 a         1083 c          949 g          479 t
ORIGIN      
        1 gtcgatctcc ggctcgacgc gcggagaatg ctcaaggtgc cgtggacggc gcacctcggt
       61 cttcacgacg acgagacctc ggagctatgc cactggcaca tccccgaagc gcacttcctg
      121 gagacgtggt cggacgcgcg cgccttcgac ggcacggcct cgatcatcca gcccctgatc
      181 gcgccgctct acaacggcaa gagcgtccac gaattcctcg tcgccttcac cgctcgcccc
      241 gaccgctcgg gctacgacat cgtgcgcgac ttctggcgca cacagatcag cggcggctcg
      301 tccgcgcccc tgctcgccgg cgcgcgcctc ttggccgcga ccgccggccg tcaggctccc
      361 gcttcacaaa cgcccgtacc cgccgtcggc ccgcagcctt ctccgggcac ctcggccgcg
      421 cagccgcagc cgacacaggg cgcacaaacg cagccgacgc cttcggcttc aacgacaccc
      481 ggcggcggct cgcccgcgac acgttcgagc ggcacgggcg cgggcgcggc cctctcggcc
      541 gccgctgccg acgacccctt cgagcgcgct tggcgcaagg ccctgcacga cggcgtcgtg
      601 cccggcacgg ccgcgcgcga gcagggccgg acggcgaacg caaacttcat caacgagaca
      661 gcgcccggcg gtacaggcgc gggcgcgtcc ccgaccgcgg ccggcgcgtc gcgcgcgacg
      721 ccggaggtcg tggtcgtcgc accgccgagc agcggcgggg caggtgagta cgaaatcgtc
      781 ttccgtcccg acccgaccat ctacgatggg cgcttcgcca acaacggctg gttgcaggaa
      841 ctcccgaaac cgctcaccaa gctgacgtgg gacaacgccg cgctcgtctc gcctaacacg
      901 gccgagcgct tgggccttgg cacgacgcct gacggcgcgc agctcgcccc ggcccgacgc
      961 gtcaaccgca tgacggcgaa gggcggcgag attgtgggcg acctcatccg catcggcttc
     1021 aagggtcaga gcctaacggt gccggcgctc atcgtgccgg gtcagcccga cggcgtcatc
     1081 acccttcatc tcggccacgg tcgctggaga gtcggacgcg tgggcgggcg cgacggcacc
     1141 ttcgagcagc cgacggggac gggcttcaac gtctataaga ttcgccactc cgacgcgctc
     1201 tggcacggcc gcggcgtcga ggtctccaaa accgaggaga cctacccgct cgccaccacg
     1261 cagattcact tccagatgga ggggcgcgac gtggtgcgct ccggctcgct cgaacagtgg
     1321 cgcgagcacc ccgagatggc gccggagacc gagcactccg tgcccgtgca gcccgccggg
     1381 aagaaccgcg agccggcgcg ccccgagtcg atgtaccccg actacgacta ctcgcaacgc
     1441 gacgagcagg gggagcgcgg ctaccgctgg ggcatggtca tcgacctcgg cgcctgcgtc
     1501 ggctgcaacg cctgcgtcat cgcctgtcag gccgagaaca acatccccgt cgtcggcaag
     1561 gagcaggttt tgcgctcgcg cgagatgcac tggctgagag ttgacgccta cttccgcggc
     1621 gcggtcgaaa accccacggg tgtccacttc atgcccgtgc cgtgcatgca ctgcgagaac
     1681 gcgccctgcg agccggtctg ccccgtccac gccaccgtgc acagcgccga gggcctcaac
     1741 gacatggtct acaaccgctg cgtcggcacg cgctactgct cgaacaactg cccctacaag
     1801 gtgcgccgct tcaacttcct gctctatcag gactgggaga cgccgtccct caagatgctg
     1861 cgtaaccccg aggtgacggt gcgctcgcgc ggcgtgatgg agaagtgtac ttactgcgtg
     1921 cagcgcatcg agtacgcgaa gatcgaggcg gagaaggagg ggccgcgccg cgtgcgcgac
     1981 ggcgagatcg tcaccgcctg tcaggccgtc tgcccctcgg aggccatcgt cttcggagac
     2041 ctcaacgacc ccgaggcccg cgtcaccaag atggcccacg agccgcgcaa ctactcgctg
     2101 ctcgccgacc tcaacacgcg cccccggacg acgtatctgg cggcggtacg caatccgaac
     2161 cccaatctag ggtgataagt gataagtgat gagtgataag agcagaagaa aagtgaaatg
     2221 aagtgaagcg atgatgggcg acgtgacaag agtgatgcgg tcgagtgtga tgagttctta
     2281 tctcatcact tatcactcat cacttatcac cgtctgagga atctatgtcc cagatcgaca
     2341 acccgcacga cgacacgcgc attcacacga cgcctgccgt catcgagccg ggccacacct
     2401 tcggctcggt gacggacaag atcagcgcca tcgtgctgcg taagcgcacg cctctggggt
     2461 ggtacatcgg gttcggcatc tccttcatga ttacgggcgt cctgatgggg acgatcctat
     2521 ggctggtctt aaacggcatc ggcatctggg gcaataacca acccgtcggc tggggcttcg
     2581 acatcatcaa cttcgtctgg tggatcggca tcggccacgc cggcacgctc atctcggcca
     2641 tcctgctcct gctccgacag tcgtggcgca cgtcgatcaa ccgcttcgcc gaggcgatga
     2701 cgctcttcgc cgtcgcctcc gccggtctgt tcccgctcct ccacaccggc cgcccgtggc
     2761 tcgcctactg gatgctgccc tacccgaaca cgatggggat gtggccgcag ttcagaagcc
     2821 ctttgatgtg ggacgtgttc gccgtctcga cttacgccac cgtctcggcg ctcttctggt
     2881 ataccggact catccccgat ctcgcgacct tgagagaccg cgcgcgctcg aagttcgggc
     2941 gcgtcgtcta cggcatggcc gctctgggct ggcgcgggag cgcgcgccac tgggcgcgtt
     3001 acgagatgac ctacctgctc ctcgctggtc tc
//
LOCUS       DASVES010000028        15793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_358372, whole genome shotgun sequence.
ACCESSION   DASVES010000028 DASVES010000000
VERSION     DASVES010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15793
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358372"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VF240_01120"
     CDS             <1..1405
                     /locus_tag="VF240_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7173871.1"
                     /translation="RSVRARTAASDADVLARLREIVGAEHVLVDAERVEPYAQDALKE
                     KFPPEAVVLPGSTREVAEVLRLANERRFPVTARGGGVGYTGGAVPVEGGIVLGTERMN
                     RVREISADDLYVVTEPGVTTYALQQAVESHGLFYPPDPASYKDSYVGGNIAENAGGIR
                     SARYGVTKHYVLGLEVVLPTGDVVRAGGRTSKNVVGFDLTGLMCGSEGMLGVITEATL
                     RLLPLPEATRTVRATFRTMREACACVSGFTRARLVPVAIEVLDRNAIRAVESEFAFGL
                     GEEAGALLLVAVVGAAPEVERSSRVVERVVTDGGGYDVIRSETREEEDRLWDVRRALS
                     PAIKKYGTLKFNEDVVVPRSRVPELVEGVEEIGRRRETFVVNFGHLGDGNIHVNFICE
                     RDDPEAVARARRAVEETFALSVSLGGTISGEHGIGYVKAPYLHLALDAPTIDAMRRIK
                     RALDPNGILNPGKMFTG"
     gene            1487..2065
                     /locus_tag="VF240_01125"
     CDS             1487..2065
                     /locus_tag="VF240_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEX7173872.1"
                     /translation="MLSVLLELQRLKALDRTGWVLRGRPAGAESVAAHSYGVALVSML
                     LADEVRARGVEVDVERVLRIAVLHDLAEARTGDMPRTMARYYGAQARRQAEGAALADL
                     LAGLDAGRARLYIELHGDYEERTGLEARLVKAADIIDLLAQALAFERAGARGLDEFWE
                     GAAERDFGLEGAAREVVAEVVGALVRAREESR"
     gene            2190..2627
                     /locus_tag="VF240_01130"
     CDS             2190..2627
                     /locus_tag="VF240_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173873.1"
                     /translation="MMDWLKQYATSFDWSWQSLLLGVGLFLVTFAASIVAVSYVLIRL
                     PPTYFQLAHARDFWADRHRAVRWSGMLVKNFFGLLLVLLGVVMSVPGVPGQGVLTILL
                     GLMLMDFPGKRQLEYKIVSRPKVMQAINRLRARFDRPPMVLDD"
     gene            2880..3959
                     /locus_tag="VF240_01135"
     CDS             2880..3959
                     /locus_tag="VF240_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173874.1"
                     /translation="MTDSNFNSKAHAPRRAALLLLLVSLAAVATGCGVFSAKRTVSVA
                     PVLAPLVKAEMAQLFAEINRAATPRSVRGKIDVQFLDTSFAECGVVDKYRTAEGDVII
                     QRPGQVYLSIKAPFGVKIAEMTSDGSKFWVAVYQGEERFRRFVHGTNAARYGRISDDG
                     NGTTTATAGDKPDCGGGKRDEKTMQRTVSSLSSLRPQHLTDALMVPPVAAAGSSLIYA
                     QSESFEEEPDTRPGAKKDARVVRGYYVLVELEPAGEGRARVTRRFWFDRVGALRLARV
                     QNYDVEGQLMTDVVYRDPRPFGAGGQQRLPATVELTRPQDRYSIRISFQTPEEAVIDK
                     QFDPDIFILQNTSDLKEVDLDKQQK"
     gene            3991..4371
                     /locus_tag="VF240_01140"
     CDS             3991..4371
                     /locus_tag="VF240_01140"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7173875.1"
                     /translation="MSESDGHIRSHRDLVAWQKAMDLVVAVYSASKTFPKEETYGLTN
                     QLRRAAVSVPANIAEGQGRRSKSEFRQFLGNARGSLLEVDTHLELAMRLGYLSAEQHT
                     HLHARLQEVGRILNGLLRSLNTDL"
     gene            4398..5498
                     /locus_tag="VF240_01145"
     CDS             4398..5498
                     /locus_tag="VF240_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7173876.1"
                     /translation="MESLVVANIRQRPVRALVSVAGVALGVCLVMLFTGLARGMSNDL
                     QRRSQNIRAEIIFTRPGGTGLVETTANLSTRYVEEILKVEGVEAAIPAIRYASQTGRG
                     FGFEQIEGVDWEPFARMNGMQLVEGRAPAALDEVVIDETKARNNEVGVGGTVNIFGNI
                     PYRVVGIYTPEAGPRVKMTLAAMQDRLQSPPDKCTWIYVKVREGASDTEVARRLDEAL
                     EGNKIQFTRDVFTSVEKSIPYLGVFLRTLVGLAAVVSALVVMLAMYTTITERTREIGI
                     LKALGASRGYIVSEIVKEAVVISLLGLVVGFAASLAAGFLIHRVYGLIFEYGWTWALA
                     AAVIGLAGGALGALYPAVRAANLDPVNALAYD"
     gene            5583..6284
                     /locus_tag="VF240_01150"
     CDS             5583..6284
                     /locus_tag="VF240_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7173877.1"
                     /translation="MRNILVADNLEMVYQSSGGVPVHALRGVNLEVEEGEYLAVMGPS
                     GCGKSTLLHLFGGLLTPTSGRILIDDEDMTAATDAQRTDIRRRKIGFVFQRFNLFPTL
                     TAEGNLRLAERIHAGKDGKIEPERRREILRMLQLENKMDRKPMELSGGEQQRVALARA
                     VVNHPAIVLADEPTGNLDTENSAIVLNMFQEMNYRFNQTIIMITHNPEAAAMCRRIIQ
                     MRDGQIVAPATRVQI"
     gene            6371..6718
                     /locus_tag="VF240_01155"
     CDS             6371..6718
                     /locus_tag="VF240_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173878.1"
                     /translation="MSPILRVIKNIVLWSYGRATWQYDVLCVLILAFVFLTPKSWFDG
                     GKPLGGALHPNSSVGVEKLLLKPDILGENPDQQAVERGVRVATGRSDVRVRGVRELRD
                     ASGRIAAYEVDIE"
     gene            6792..7520
                     /locus_tag="VF240_01160"
     CDS             6792..7520
                     /locus_tag="VF240_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX7173879.1"
                     /translation="MKKFVTLGLIAMLALATTAVVPSAASAKPAPAAPQGAGLVSSIL
                     NKMERNRRELKSLRAGVWMQKYNAQIKDEDNFVGDAHYIPGAGREVALRVDWSKPQQE
                     TLIVEGGTYTLFKPRMNMAYKGSTSSASKNNKVTNVIGFGLNVSKAELNAKFQPVELL
                     GEGTLETGGAHVWWLKLVPKGNAGYEHAEIWVDDSGMPRQVRIVERNKDMTTVRLINP
                     QKNAPVSAGTFKIDLGSGVKIVKG"
     gene            7785..8798
                     /locus_tag="VF240_01165"
     CDS             7785..8798
                     /locus_tag="VF240_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7173880.1"
                     /translation="MKRSTFLKLKLSEAQFIARALLKTSHPVLAHVIPMRRCNLACAY
                     CNEYDRTSDPVPLEEMRRRLDKLADLGTSIITLSGGEPMMHPDLDEIVAHIRRRRMIA
                     GLITNGFYLNPKRIERLNRAGLKYLQISIDNVEPDDVSMKSLKLLEKRLGWLADGADF
                     HVNINSVIGAGIKNPEDALTVARRALELGFSTTVGVIHDGQGLLKPLSEHERQVFEEI
                     KRISKTEYARLGWFQNNLAEGKPNEWRCRAGARYLYICEDGLVHYCSQQRGTPGIPLA
                     DYTSADVEREYNTEKWCAPFCTVACVHKVSILDNWRDPQQGTQNAAPQKLRDAIGRLL
                     RAE"
     gene            8892..9914
                     /locus_tag="VF240_01170"
     CDS             8892..9914
                     /locus_tag="VF240_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7173881.1"
                     /translation="MPLLRPVSALFLILSLPFACQGGVAWPGARAFGQTPAASPASPA
                     LPAGGDRVRRNLYVSVVDKDTRFVEGLSAETFVVQDGKERAEVVSFAKADVPTSVGFL
                     LDMSGSMFYQERKKIVVKERADVIRNAVTHFLEASHPANDYFVISFNQMHKELLDVTN
                     DRAAVLAAVESIAANPLKGRTALYDTCRLALDKLARSTKARRVLVIFTDGQDNASRAD
                     FDDLKRALKQSDVLVYPLAFFSPHYDSFLHEEAQRILDEFAQISGGIAYTTRDMKEIE
                     PMFAHVAQGLRMQYALEVGLAPSVRKDGWRELKVKLAEMRDKEGKTHKIFPRTRKGFY
                     LPVAAR"
     gene            complement(9930..10190)
                     /locus_tag="VF240_01175"
     CDS             complement(9930..10190)
                     /locus_tag="VF240_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173882.1"
                     /translation="MRKLLGLELSKAGRWEEALAELLAAERGGEPDDLIHFHIAAALA
                     ATGRTTEAASEYQRFLQAPACARALPDPRCDAARTFLHKFAE"
     gene            complement(10620..12059)
                     /gene="pyk"
                     /locus_tag="VF240_01180"
     CDS             complement(10620..12059)
                     /gene="pyk"
                     /locus_tag="VF240_01180"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX7173883.1"
                     /translation="MRRAKILATLGPASREPAVLESLLAAGAGAVRINMSHGTQEEHV
                     ETIARARAAAERMKRPLAVLVDLSGPKIRTGVLRNGQPVQLENNAIFTITTRSVAGDA
                     REVSTNYAGMARDVRAGMRLLIDDGLIELVAESTTETDVICRVINGGPLGERKGINLP
                     GAKLPIPSLTDKDRRDLQWAVRQRVDYIALSFVRRADDCREAKSLIEAAGGAQPLVAK
                     IEKSEAIDNLDDIISAADGVMVARGDLGVETSVELVPVYQKRIIASANNAGKVVITAT
                     QMLQSMINEPRPTRAEATDVANAVWDGSDAVMLSAETATGQFPVLAVATMARIIESAE
                     TGIPTGSSDIAKWAGKQSGRVSRALCEAAAFAAEELNVKVVAIFTESGLMARRLAALR
                     PSQRIIALTPRREVQNELSLVWGVEAIVHEPGDNTDDMMRLGERTLLEAGAVQQGDML
                     VIMAGRLSGLGLSSSVSVHTIGEQVPRQL"
     gene            complement(12280..12936)
                     /locus_tag="VF240_01185"
     CDS             complement(12280..12936)
                     /locus_tag="VF240_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7173884.1"
                     /translation="MSFAPTRLCAPPRRRAPRLLLRLAPVFCLLALSGCGEAPRYARS
                     EVGTATPEATRSTATLPRTNLPMPPIASSHGAATQAPGWTLLDGRRESLADYQGRVLV
                     LDLYATYCPPCRDEIPQLISLQRRYEARGLKVIGLNVGGDDDRRLVPDYVRELGIDYA
                     LGNPDDELINTLSGGETAIPRTYVFDRRGRLADFSVGYDNEVAARLEQAVQKAVASDE
                     "
     gene            complement(13107..13316)
                     /locus_tag="VF240_01190"
     CDS             complement(13107..13316)
                     /locus_tag="VF240_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX7173885.1"
                     /translation="MKKCPVCNKPVEWQGNPHRPFCSERCKLIDFGGWVNGEYAVPTD
                     EAPPFEEMDGQPLTSGDGSDRPSGA"
     gene            complement(13437..14156)
                     /locus_tag="VF240_01195"
     CDS             complement(13437..14156)
                     /locus_tag="VF240_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7173886.1"
                     /translation="MSHRRACDLTTEERAEIILHSEIGPRLGGELLARLAHASSALDV
                     RRRRFIYRRGDQADTLYLIARGRVKICSIEDETAREAVIDIIGPGALFGESALYGPGA
                     RERSAVAYENARLIRIPVGEFREAMSGDRELYDCTFRLVGQRLSRAERRMADFALDAI
                     PARLDKLLVELSERYGRAEAGGVLIDLNLPHREIASIVGSTRESVTVRLNAMRRAGII
                     DFVDRKILIRTPAASAVASLR"
     gene            14468..15400
                     /locus_tag="VF240_01200"
     CDS             14468..15400
                     /locus_tag="VF240_01200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7173887.1"
                     /translation="MMGKFKILIVDDEPDKLEMLTVALRMEGYTVHTAVDGVTGLAVV
                     EARHPDLIITDVMMPRMDGYEMARRVRQNPLTRFIPIIIQSAARHEPQDIRMGFEIGA
                     LGYFTDPSDIPLLLARTRTLLEFKNYLDSCEEAAFTDHLTGLANRRRFERQLGREVER
                     SRRYGHNVCLLLADIDNFKSVNDTYGHEAGDEALRAVANVLQSGTRGIDTGARLGGDE
                     LAVLLPETGFKRGMEVAERLRAAVASLGIPASGGVTASFGLAEFPTCARDERELFAAA
                     DQALYEAKRQGRDCVRCAQPSAPEPNPAPALGVS"
     gene            15510..>15793
                     /locus_tag="VF240_01205"
     CDS             15510..>15793
                     /locus_tag="VF240_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173888.1"
                     /translation="MADYKDRLDEWRDAARRKARELDEKYGIKDRVEEGARVASDAAR
                     RGADKVAGEAARAREEAERLAEDFDLRERADEVRTHAERAAEEATRRAREA"
BASE COUNT         2822 a         5067 c         5182 g         2722 t
ORIGIN      
        1 ccgtagcgtg cgcgcgcgca cggccgcctc ggacgctgat gtgctggcgc gcctgcggga
       61 gatcgtcggc gcggagcatg tgctcgtcga tgcggagcgg gtcgagcctt acgcgcagga
      121 cgcgctgaag gaaaagtttc cgcccgaggc cgtggtgctg cccggctcga cgcgcgaggt
      181 cgcggaggtg ctgcggctcg ccaacgagcg gcgcttcccc gtcacggcgc gcggcggcgg
      241 cgtcggctac acgggtgggg ccgtgcccgt cgagggcggc atcgtgctgg gcactgagcg
      301 catgaaccgt gtccgcgaaa tcagcgccga cgacctctac gtcgtgaccg agccgggcgt
      361 gacgacctac gcgctgcaac aggcggtcga gtcgcacggc ctcttctacc cgcccgaccc
      421 ggcctcctac aaggactcct acgtcggcgg caacatcgcc gagaacgccg gcggcatccg
      481 ctccgcccgc tacggcgtga cgaagcacta cgtgctcggc ctcgaagtgg tgctccccac
      541 cggcgacgtg gtgcgcgcag gcggacggac gagcaagaac gtcgtcggct tcgacctgac
      601 ggggctgatg tgcggctccg aggggatgct cggcgtcatc acggaggcga cgctccggct
      661 cctgccgctc cccgaggcga cgcgcaccgt gcgcgccacc ttccgcacga tgcgtgaggc
      721 ttgcgcctgc gtctccggct tcacgcgcgc gcggctcgtg cccgtcgcca tcgaggtgct
      781 ggatcgcaac gccatccgcg ccgtcgagtc ggagttcgcc ttcgggctgg gcgaggaggc
      841 gggggcgttg ctcctcgtcg ccgtggtcgg cgcggccccc gaggtcgagc gctcctcccg
      901 cgtcgtcgag cgcgtggtca ctgacggcgg cggttacgac gtgatccgct cggagacgcg
      961 cgaggaggaa gaccgcctgt gggacgtgcg gcgcgcgctc tcgcccgcga tcaaaaagta
     1021 cggcaccttg aagttcaacg aggacgtggt cgtgccgcgc tcgcgcgtgc ccgaactcgt
     1081 cgaaggcgtc gaggagattg gccggcggcg cgaaaccttc gtcgtcaact tcggccatct
     1141 tggcgacggc aacatccacg tcaacttcat ctgtgagcgc gacgaccccg aggccgtcgc
     1201 ccgcgcccgc cgggccgtcg aagagacctt cgcgctgtcc gtctccttgg gcggcaccat
     1261 cagcggcgag cacggcatcg gctacgtcaa agccccctac ctccatctgg cgctcgacgc
     1321 cccgaccatc gacgccatgc gccgcatcaa acgcgccctc gaccccaacg gcatactcaa
     1381 tccggggaag atgtttacag ggtgataagt gatgagtgat aagtgataag agaagagctg
     1441 atttccttct tatcacttat cactcatcac ttatcacttt agaaaaatgc tcagtgtcct
     1501 tctcgaactg caacgcctca aggcgctcga ccgcacgggc tgggttttgc gcgggcggcc
     1561 ggcgggggcc gagtccgtgg cggcgcactc ttacggggtg gcgctcgtgt cgatgctgct
     1621 ggccgatgag gtgcgcgcac gcggcgtcga ggtggacgtg gagcgcgtgc tgcgtattgc
     1681 cgtgctgcac gatctggcgg aggcgcgcac cggcgacatg ccgcgcacga tggcgcgata
     1741 ctacggcgcg caggcgcgca ggcaagccga gggcgcggcc ttagccgacc tgcttgccgg
     1801 actcgacgcc gggcgcgcgc gtctctacat tgaactgcac ggcgactacg aggagcgcac
     1861 ggggcttgag gcgcggctcg tgaaggccgc cgacatcatc gacctgctgg cgcaggcgct
     1921 tgccttcgag cgcgccggcg cacgcggcct cgacgagttc tgggagggcg cagccgagcg
     1981 cgactttggg cttgagggcg cggcccgcga ggtcgtcgcc gaagtcgtcg gcgctctcgt
     2041 cagggcgcgt gaggaaagca ggtaggaaga ttcttacatg gatggacaga aagatgtgat
     2101 gagtgatgag tgataagtga taagaaagag cagacggttt ttctcatcac tcatcactca
     2161 tcactcatca ctcttctttt gggcacggga tgatggactg gctgaaacaa tacgcgacct
     2221 cgtttgactg gagctggcag agcctgctgc tgggcgtcgg actattcctc gtcacgttcg
     2281 ccgccagcat cgtggccgtc tcctacgtgc tcatccggct cccgcccacc tacttccagc
     2341 tcgcacacgc gcgcgacttc tgggccgacc gtcaccgcgc cgtccgctgg agcggcatgc
     2401 tcgtcaaaaa cttctttgga ctcctgctcg tcctcctcgg cgtcgtcatg tcggtgccgg
     2461 gcgtgcccgg acagggcgta ctcactatcc tgctcggcct catgctcatg gatttccccg
     2521 gcaagcgcca gctcgaatac aaaatcgtca gccgccccaa agtcatgcaa gccatcaacc
     2581 gcctgcgtgc ccgtttcgac cggccgccga tggtgctgga cgactaaaag ccgtaaatcg
     2641 tgtatcgtca atcggagaag agctttcagc tatcagccgt cagctctcag ccaaccgcag
     2701 gcgttgttag gccgattgac cctgacaccc gaaagccctt cgttcgctct attctggctg
     2761 aatgctgaaa gctgaaagct aatagctgag ttctgaccgc gttgacaagg gaagggtgct
     2821 ttgttagctt ccaaaaatta tcaatcggat gtgcccgtgt ggttcgtaca gttaagtcga
     2881 tgactgactc caatttcaac agtaaggcgc acgcgccgcg tcgcgcggcg ctgctgctcc
     2941 tgctcgtctc gctggcggcg gtggcgaccg gctgcggcgt cttcagcgcg aagaggacgg
     3001 tgagcgtcgc gccggtgctc gcgccgctcg tgaaggccga gatggcgcag ctcttcgccg
     3061 agatcaatcg cgcggccacg ccccggagcg tgcgcggcaa gatcgacgtg cagttcctcg
     3121 acacgtcctt cgccgagtgc ggcgtcgttg acaagtaccg cacggccgag ggcgacgtaa
     3181 tcatccagcg ccccggtcag gtctatctct ccatcaaggc ccccttcggc gtcaagatcg
     3241 ccgagatgac ctcggacggc agcaagttct gggtcgccgt ctatcagggc gaagagcgtt
     3301 tccgccgctt cgtccacggc acgaacgcgg cccgctacgg ccgcatctcg gacgacggca
     3361 acggcactac gacggcgacg gccggcgaca agcccgactg cggaggcggc aagcgcgacg
     3421 agaagacgat gcagcgcacg gtcagctcgc tctcctcctt gcgcccgcaa cacctgaccg
     3481 atgcgctcat ggtgcccccc gtggcggcgg ccggctcgag cctgatctac gcgcagtcgg
     3541 agtcgttcga ggaggagccg gacacgcgcc ccggggcgaa gaaggacgcg cgcgtcgtgc
     3601 gcggctatta cgtcctcgtg gagctggagc cggcgggcga ggggcgcgcg cgcgtcacgc
     3661 gccgcttctg gttcgaccgc gtcggcgccc tccgcctcgc gcgcgtgcag aattacgacg
     3721 ttgaggggca gctcatgacg gacgtggtct atcgcgaccc gcgccccttc ggcgccgggg
     3781 gccagcagcg ccttccggcc acggtggagc taacgcgccc gcaggatcgc tactcgattc
     3841 gcatcagctt ccagacgccc gaagaagcag tcatcgacaa gcagttcgac cccgacatct
     3901 tcatcctcca gaacacctcc gacctgaagg aggttgattt ggacaagcag cagaaataga
     3961 ggtcagaggt tagaggttag aggttagtta atgagcgagt ccgacggcca catcaggagc
     4021 catagggact tggtggcatg gcagaaggcg atggatttgg tcgtcgcggt ctatagcgcc
     4081 agtaaaactt tcccgaaaga agagacctac ggcttaacga atcagttgcg gcgtgcggcc
     4141 gtttccgtac ctgctaacat cgctgagggt caggggcgga ggtcaaaaag tgaattccgt
     4201 cagttcctgg gcaacgccag gggctcgctt ctggaagtgg atactcatct ggaactggca
     4261 atgcgcctcg gatacctaag cgcggaacag cacactcatt tacacgcgcg gttgcaagag
     4321 gtcggacgta tactcaacgg gcttctacgc tcgctaaata ctgacctcta acctctaacc
     4381 tctgacctct tgtttttatg gaaagcctcg tcgtcgccaa catcagacag cggcccgtgc
     4441 gcgcgctcgt gagcgtggcg ggcgtggcgc tgggcgtgtg tctggtgatg ttgtttacgg
     4501 ggctggcgcg cgggatgtcg aacgaccttc agcggcgctc gcagaacatc cgcgccgaga
     4561 tcatcttcac gcgccccgga gggacggggc tggtggagac gacggccaac ctgagcacgc
     4621 gctacgtcga ggagatactc aaggtcgagg gcgtggaggc ggccatcccg gccatccgct
     4681 acgcgtcgca gacggggcgc ggtttcggct tcgagcagat cgagggcgtt gactgggagc
     4741 cgttcgcgcg gatgaacggg atgcagctcg tcgaggggcg cgcgcccgcg gcgctggacg
     4801 aggtggtcat tgacgagacg aaggcgcgta acaacgaggt cggcgtcggc ggcacggtca
     4861 acatcttcgg caacattccc tatcgcgtcg tcggcatcta cacgccggag gccgggccgc
     4921 gtgtgaagat gacgctggcc gcgatgcagg atcggctcca gtcgccgccg gacaagtgta
     4981 cgtggattta cgtgaaggtt cgcgagggcg cgtccgacac ggaggtggcg cggcgtctgg
     5041 atgaggcgct cgaggggaat aagattcagt tcacgcgcga cgtgttcacg agtgtggaga
     5101 aaagcatccc ctatctcggc gttttcctgc gcacgctggt cgggctggcg gcggtggtga
     5161 gcgcgctcgt cgtgatgctg gcgatgtaca cgaccatcac ggagcggacg cgcgagatcg
     5221 gcatcctcaa ggcgctgggc gcttcgcgcg gctacatcgt cagcgagatc gtcaaggagg
     5281 ccgtggtcat cagcctgctc ggcctcgtcg tcggcttcgc ggcctcgctc gccgccggct
     5341 tcctgattca ccgcgtctac ggtctcatct tcgagtacgg ctggacgtgg gcgctcgcgg
     5401 cggccgtcat cggtctcgcg ggcggcgccc tcggcgcgct ctacccggcc gtgcgcgccg
     5461 ccaacctcga cccggtcaac gctctcgcat atgactagtg ataagtgatg agtgataagt
     5521 gataagataa aagacgctct ttaacttatc acttatcact catcacttat cactcttttt
     5581 gtatgagaaa cattctcgtc gccgacaacc ttgagatggt ctaccagagc agcggtgggg
     5641 tgccggtgca cgcgctgcgc ggggtcaatc tggaggtcga ggagggggag tatctggcgg
     5701 tgatggggcc ttccgggtgc gggaagtcaa cgctgctgca cctgttcggg ggactcctga
     5761 cgccgacctc cgggcgcatc ctcatcgacg acgaggacat gacggccgca acggacgccc
     5821 agcgcacaga catccgacgc cgcaagatcg gcttcgtctt ccagcgcttc aacctcttcc
     5881 ccacgctcac cgccgagggc aatctgcgtc tcgccgagcg catccacgcc ggcaaggatg
     5941 ggaagatcga gccggagcgc cgccgcgaaa tcctgcggat gctccaactc gaaaacaaga
     6001 tggatcgcaa gccgatggag ctttcggggg gcgagcagca gagagtggcg ctggcccgcg
     6061 ccgtcgttaa ccacccggcc atcgtcttag ccgacgaacc caccggcaac ctcgacacgg
     6121 agaactcggc catcgtcctc aacatgtttc aggagatgaa ctaccgcttc aaccagacca
     6181 tcatcatgat cacccacaac ccggaggccg ccgccatgtg ccgccgcatc atccagatgc
     6241 gcgacggcca gattgttgcc cccgccacgc gtgtacagat ataatgcagg tggtaagtga
     6301 tgagtgatat gtgataagag aaacactgat tttcttatca ctcagcactc atcactcatc
     6361 actaaccctt atgtccccca tcctgcgcgt catcaaaaac atagtcctgt ggtcttacgg
     6421 gcgcgccacg tggcaatacg acgtgctgtg cgtcctcatc ctcgccttcg tcttcctgac
     6481 acccaaaagc tggttcgatg gcggcaaacc gctcgggggg gcgttgcatc caaattcatc
     6541 agttggagtg gaaaagttgc tcctgaagcc cgacattctg ggcgaaaacc ccgaccagca
     6601 ggcagttgag cgcggcgtgc gagttgcaac aggacggtca gatgtacgcg tcaggggggt
     6661 tagagaactg agggacgcgt cgggtcgtat tgccgcttat gaggttgaca tagagtaatc
     6721 atctacagat aatcgcccgg tttggttccg ggcgagagag ttcggtctcc caactttccg
     6781 aaggagttcg catgaagaag ttcgttacgc ttggcctgat cgcgatgctg gcgctcgcca
     6841 ccacggccgt ggttccctcg gccgccagcg caaaaccggc cccggccgcc ccacagggcg
     6901 cgggcctcgt cagctccatc ctcaacaaga tggagcgtaa ccgccgcgag ctgaagagcc
     6961 tgcgcgcggg cgtctggatg cagaagtaca acgcgcagat caaggacgag gacaacttcg
     7021 tcggcgacgc ccactacatc cccggcgcgg gccgcgaagt cgcgttgcgc gtggactgga
     7081 gcaagccgca gcaggagacg ctgatcgtcg aaggcggcac gtacacgctc ttcaagccgc
     7141 gcatgaacat ggcctacaag ggcagcacca gctcggcgtc gaaaaacaac aaggtgacga
     7201 acgtcatcgg cttcggcctc aacgtctcga aggccgaact caacgccaag tttcagcccg
     7261 tcgagctgct cggcgagggc acgctcgaaa cgggcggcgc gcacgtctgg tggctgaagc
     7321 tcgtgccgaa gggcaacgcc ggttatgagc acgccgagat ttgggtggac gacagcggca
     7381 tgccgcgtca ggttcgcatc gtcgagcgca acaaagacat gacgaccgtg cgcctgatca
     7441 acccgcagaa gaacgcgccc gtctcggccg gcacgttcaa gatcgacctc ggctcgggcg
     7501 tgaagatcgt caagggctaa aggggcggtc ggcccgtcaa agctgcacac ccggaggttt
     7561 tcggacgctg ggaagtttcc gaaaccctct tcgtttatca ggcggagtgg gacaaaaatt
     7621 tctgcctgtc atccgtttgt gagacgggca ggaatgcctg tcccaacttc cgggtgcttg
     7681 cttcggggcg cgcacttcgc gtattttcta gcgcgactac taaagacgaa cacgggcgca
     7741 tccaaacact ttcccacgcc ggccgtcggc cgccgaaagc cttgatgaag aggagcacat
     7801 ttcttaagct caagctgagc gaggcgcagt tcatcgcgcg cgctctgctg aagacgagcc
     7861 atcccgtgct ggcgcacgtc atcccgatgc gccgctgcaa cctcgcctgc gcctactgca
     7921 acgagtacga ccgcacgtcc gaccccgtgc cgctcgaaga gatgcggcgc cgcctcgaca
     7981 agctcgccga cctcggcacc tccatcatca cgctctcggg cggcgagccg atgatgcacc
     8041 ccgacctgga cgagatcgtc gcgcacatcc gccgccgacg gatgatcgcc ggcctcatca
     8101 ccaacggctt ctatctcaac ccgaagcgca tcgagcgcct caaccgcgcc ggtctcaagt
     8161 atctccagat cagcatcgac aacgtcgagc ctgacgacgt gtcgatgaag agcctcaagc
     8221 tccttgagaa gcggctcggc tggctcgccg acggggccga ctttcacgtc aacatcaact
     8281 ccgtcatcgg cgccggcatc aagaatcccg aggacgcgct caccgtcgcc cgccgcgccc
     8341 tcgaactcgg cttctcgacc acggtcggcg tcatccacga cggtcagggt ctcctcaaac
     8401 ccttaagcga gcacgagcgt caggtcttcg aggagatcaa gcggatcagc aagaccgagt
     8461 acgcgcgtct ggggtggttc cagaacaacc tcgccgaggg caagcccaac gagtggcgct
     8521 gccgcgccgg cgcgcgctat ctctacatct gcgaagacgg ccttgttcac tactgctcgc
     8581 agcagcgcgg cacgcccggc atcccgctcg ccgattacac gtcggccgac gttgagcgcg
     8641 agtacaacac cgagaagtgg tgcgcgccct tctgcacggt cgcctgcgtc cacaaagtct
     8701 ccatcctcga caactggcgc gacccgcagc agggcacgca gaacgccgcc ccgcagaaac
     8761 tccgcgacgc catcggccgc ctcctccgcg ccgaataaca atagctgacg gcgaccgacg
     8821 gcgacccggc ccgccgtcgc cccgcgccgc ccccgacgcc gacccgcgat ccgacccgac
     8881 ggagcaaact catgcctctc ctgcgtcctg tctccgcgct cttcctgatt ctgagcttgc
     8941 cgtttgcgtg tcagggcgga gttgcatggc cgggcgcgcg tgcgttcgga cagacgccgg
     9001 cggcctcgcc ggcttcgccc gcgctccccg ctggcggcga ccgtgtccgg cgcaacctct
     9061 acgtctccgt ggtggacaag gacacgcggt tcgtcgaggg cttgtcggcc gagacttttg
     9121 tcgtgcagga cggcaaggag cgcgcggagg tcgtctcctt cgcgaaagcg gacgtgccga
     9181 ccagcgtcgg cttcctgctc gacatgtcag gctcgatgtt ttatcaggag cggaaaaaga
     9241 tcgtcgtcaa ggaacgggcc gacgtgatca gaaacgcagt cacgcatttc cttgaagcca
     9301 gccacccggc gaatgactac ttcgttatct ccttcaacca gatgcacaaa gagttgctcg
     9361 acgtgacgaa cgatcgcgcc gccgtgctgg ccgccgtcga gtccatcgcc gccaacccgc
     9421 tcaagggccg gacggcgctc tacgacacct gccgcctcgc gctcgacaaa ctcgcgcgct
     9481 cgacgaaggc gaggcgcgtg ctcgtcatct tcaccgatgg ccaggacaac gcctcgcgcg
     9541 ctgactttga cgacctgaag cgcgcactca agcagagcga tgtactggtc tacccgctcg
     9601 ccttcttcag cccacactac gattcctttc tccacgagga ggcgcagcgc atcctcgacg
     9661 agttcgccca aatctccggc ggcattgcct acaccacgcg cgacatgaag gagattgagc
     9721 cgatgttcgc gcacgtcgcg caggggttgc gtatgcagta cgcgctcgaa gtcggcctcg
     9781 ccccgtcggt caggaaggac ggctggcgcg agctgaaggt gaagctcgcg gagatgcgcg
     9841 acaaggaggg caagacgcac aaaatatttc ctcgcacccg caaaggcttc taccttccgg
     9901 tcgccgcacg ctgacacggc cgcttaaaat cattccgcaa atttgtgtaa gaaggtgcgt
     9961 gccgcgtcgc agcgcgggtc ggggagcgcc cgtgcgcagg cgggcgcctg aaggaaacgc
    10021 tggtattcgc tggcggcctc ggtggtgcgc cccgttgcgg cgagtgcggc ggcgatgtgg
    10081 aagtggatca agtcgtcagg ctcgccgccc cgttcggccg cgagcaactc ggcaagcgcc
    10141 tcctcccagc ggcccgcctt cgacaactcc agccccagaa gctttcgcac gtgcgggtcg
    10201 tcctctcgcc gccggagaac tgtccacagc ctcgttcgtc actccgtctg tcactccgta
    10261 agacgcgccg ggcggggcga cggcgaccgc gtggccgtca actgtgtcgg tcgtcacggc
    10321 gggcaagcgg tcgttcgtct cgttgtcttc atcgttcgcg tcagccggcg cttcttctgc
    10381 cgccgtcgcc gacgggcgtg cataggcgcg cgcgatgagg tagaggggaa gcgtgacgtg
    10441 tgggagaaag agtgtgaagg ccgtccagag ggcgaccgcg cggggtcgga aatacgagcg
    10501 cgagtcggcg agcacgccgg cggaggcgag cgcggctgtg gcgaagaggg cggggggcgc
    10561 gcgcctccgc gaggaagccg gcggagattt gccagtcggc gctcaaaggt ttcgatcctt
    10621 cagagctggc gcggcacctg ctcgccgatg gtatgcaccg agacggaact cgaaagcccc
    10681 agccccgaga gccggcccgc catgattacc agcatgtcgc cctgctgtac ggcgcccgcc
    10741 tccagcagcg tgcgctcgcc aagccgcatc atgtcatccg tgttatcccc cggctcgtgg
    10801 acgatggcct ccacacccca gacgagcgac agctcgttct gcacctcgcg gcggggcgtg
    10861 agcgcgatga tgcgctgact gggacggagc gcggcgagac gacgggccat cagacccgac
    10921 tcggtgaaga tggcgactac cttcacgttc aactcctcgg ccgcgaacgc cgccgcctcg
    10981 cacagcgcgc ggctgacgcg ccccgactgc ttgccggccc acttcgcgat gtcgctcgac
    11041 cccgtgggaa tgcccgtctc ggccgactcg atgatgcgcg ccatcgtcgc cacggcgagg
    11101 acggggaact gccccgtggc cgtctcggcc gagagcatca cggcgtcgga gccgtcccac
    11161 acggcgttgg ccacgtcggt ggcctcggcc cgggtcgggc gcggctcgtt gatcatcgac
    11221 tggagcatct gcgtcgccgt gatgacgacc ttgcccgcgt tgttggccga cgcgatgatg
    11281 cgcttctggt agacgggaac caactcgacc gaggtctcca cgccaaggtc gccgcgcgcg
    11341 accatcacac cgtcggccgc actgatgatg tcgtcgaggt tgtcgatggc ctccgacttc
    11401 tcgatcttgg cgacgagcgg ctgcgcgccc ccggcggcct cgatgagcga cttggcctcc
    11461 cggcagtcgt cggcgcggcg gacgaaagag agcgcgatgt agtcaaccct ctgccgcacg
    11521 gcccactgca agtcgcgccg atccttgtcg gtgagtgagg ggatggggag cttcgcgccg
    11581 ggcaggttga tgcccttgcg ctcgccgagc ggcccgccat tgatgacgcg acagataacg
    11641 tccgtctcgg tcgtgctctc ggcgacgagt tcgatgaggc cgtcgtcgat caggaggcgc
    11701 atgccggcgc gcacgtcgcg ggccatgccg gcgtagttcg tggagacctc gcgcgcgtcg
    11761 ccggccacgg agcgcgtggt gatggtgaag atggcgttgt tctcaagctg gacgggctgg
    11821 ccgttcctga gcacgcccgt cctgatcttc ggccccgaca gatcgacgag caccgcgagc
    11881 gggcgcttca tgcgctcggc ggcggcgcgc gcgcgggcta tcgtctcgac gtgctcctcc
    11941 tgcgtgccgt gcgacatgtt gatccgcacg gcgcccgcgc ccgccgccag caacgattcc
    12001 aacaccgccg gctcgcgcga cgccggcccc agcgtggcaa gtatcttcgc tctcctcaca
    12061 cctgcacccc ttgtctgacg aaatcgaatt gttcgggaat ggctgttgtt ctaataactc
    12121 gtaaagacgt gaatcgtcaa tcatgggcga gcggttagca gttagctttc agcttttagc
    12181 tcatggcagg cgtcgtcgcg tgcgtggccg acagcaatca ataccctggt tcgctctctt
    12241 tagctaaaag ctaaaagcta accgctccct tcgcggcttt cactcgtcgc tcgcgacggc
    12301 cttctgcacc gcctgttcga gccgggcggc gacctcgttg tcgtagccga cggagaagtc
    12361 ggcgagacgg ccccggcggt cgaagacgta ggtgcgcggg atggcggtct cgccgccgga
    12421 gagggtgttg atgagttcgt cgtcgggatt gccgagggcg tagtcgatgc cgagttcgcg
    12481 gacgtagtcg ggcacgaggc ggcggtcgtc gtcgccgccg acgttgaggc cgatgacctt
    12541 gaggccgcgc gcttcgtagc gccgctggag cgagatgagt tgcgggattt cgtcgcggca
    12601 gggcgggcag taagtggcgt agaggtcgag gacgaggacg cgcccctgat agtcggcgag
    12661 cgactcgcgg cggccgtcga gcagcgtcca gccgggcgcc tgcgtggccg cgccgtgcga
    12721 ggaggcgatg ggcggcatcg gcaggttcgt gcgcggcagc gtcgccgtcg agcgagtcgc
    12781 ctccggtgtc gccgtgccca cctccgagcg cgcgtaacgc ggcgcttctc cgcagcccga
    12841 gagggcgagc agacaaaaga caggcgccag ccgtagcagc agacgcggcg cgcggcggcg
    12901 cggcggcgca caaagacgcg tgggcgcgaa actcatctcg gtcacaacac tccgcatgac
    12961 gaccatttta cacgacgggc aggaaggcgg cacgagtcgg caggccacag acttgctttt
    13021 cttacttacc taacgtttag aggcgggcat tgcccgcctc taaacgttag aagtttttta
    13081 ttgagaatcg cgttgattgt cagccatcac gcgccggagg gccggtcgga gccatcgccg
    13141 gaggtgagcg gctggccgtc catctcctcg aagggcgggg cctcatcggt gggcacggcg
    13201 tactccccgt tgacccagcc gccgaagtcg atcaacttgc accgctctga gcagaagggc
    13261 cggtgtgggt ttccctgcca ctcgacgggc ttgttgcaaa cgggacactt cttcatagcc
    13321 gtcaatcgtc aatcgtgaat cgtgaaagtt gttgctgccc tggcctgaga ttgtaccatt
    13381 cgggccgggc tgccgctcaa ccgcgattga cgatccacga ttttcgattt acggctttat
    13441 cgaagtgacg ctacggcgct ggcggccggg gtgcggatta aaatttttcg gtcaacgaag
    13501 tcgatgatgc cggcgcggcg catggcgttc aagcggacgg tgacgctctc gcgcgtggag
    13561 ccgacgatgg aggcgatctc gcggtgtggc aggttgaggt cgatgagcac gccgccggcc
    13621 tcggcgcggc cgtagcgttc ggagagttcg acgaggagct tgtcgagacg ggccgggatg
    13681 gcgtcgaggg cgaagtcggc catccgccgc tcggcacggg aaagccgttg accgacaagg
    13741 cggaaggtgc aatcataaag ctcgcgatcc cccgacatcg cctcgcgaaa ctcccccaca
    13801 ggaatgcgta tcaggcgcgc gttctcgtag gccacggcgc tgcgctcgcg cgcacccggc
    13861 ccgtagaggg ccgactcgcc gaagagcgcg ccggggccga tgatgtcgat aacggcctcg
    13921 cgggcggtct cgtcttcgat ggagcagatt ttgacgcggc cgcgggcgat gaggtagagc
    13981 gtgtcggcct ggtcgccgcg gcggtagatg aagcggcggc ggcgcacgtc gagggcggag
    14041 gaggcgtgcg ccagacgggc gagcagttcg ccgcccagtc gggggccgat ctcggagtgg
    14101 aggatgatct cagcgcgttc ttccgtggtc agatcgcagg cgcgtcggtg tgacatgatc
    14161 gggggcctcg ggcgatgccg tcgcctcggg aaaattttac aggagtcggc ggaattcttg
    14221 cgcgcgcggg atgtacgtgt cccggcggaa accctttgac tcggctgacc tgagtggatt
    14281 tttacagatg cggaaaacga ttttagaagc gcgaggtgat tttattgatt tcgcgtgcgt
    14341 ccgaatgagg acgagggcaa gctttcgaac agacgcgaac gaacccttca accttgctga
    14401 cgacgcactc tagcatgaag cagcgggggg tgcaagggat gtttttttga ggccgaacga
    14461 acctggcatg atgggaaaat tcaaaattct gatcgtcgat gacgagccgg ataagctgga
    14521 aatgctgacc gtcgcgctcc gcatggaggg ctacaccgtc cacacggccg tggacggcgt
    14581 gaccggatta gccgtggtcg aggcgcgtca tcccgacctc atcatcaccg acgtgatgat
    14641 gccccggatg gacggctacg agatggcccg gcgcgtccgc cagaacccac tgacgcgctt
    14701 catcccgatc atcatccagt cggccgcgcg ccacgagccg caggacatcc gcatgggttt
    14761 cgagatcggc gcgctcggct acttcacaga cccttcggac attcccctac tcctcgcccg
    14821 cacgcgcacg ctcctcgaat tcaaaaacta cctcgacagt tgcgaggagg cggccttcac
    14881 cgatcacctg acgggactgg ccaaccgccg ccgcttcgag cgccagttgg gccgcgaggt
    14941 ggagcgcagc cgccgctacg gccacaacgt ctgcctgctt ctggccgaca tcgacaactt
    15001 caagagtgtg aacgatacct acggccacga ggcgggcgac gaggcgctgc gggcggtggc
    15061 gaacgtcttg cagtcgggca cgcgcggcat cgacacgggc gcgcgcctcg gcggcgacga
    15121 gctggccgtg ctcctgcccg agaccggctt caaacgcggc atggaggtgg ccgaacgcct
    15181 gcgcgcggcc gtcgcctcgc tgggcatccc cgcgtccggc ggcgtcacgg ccagcttcgg
    15241 tctggccgag ttcccgacct gcgcgcgcga cgagcgcgag ctgttcgccg ccgccgatca
    15301 ggccctctac gaggccaagc gacagggacg cgactgcgtc cgctgcgcgc agcccagcgc
    15361 gcccgagccg aacccggcgc ccgcgctggg cgtatcataa agcggcagaa gtcaggagtc
    15421 aggagtcaga ataaacacat gtcgctctct tcattctgac ttctgacttc tgacttctga
    15481 ctcccgattt tcttctgaaa ggttgagtaa tggctgatta caaggacagg ctggacgagt
    15541 ggcgcgatgc ggcgcggcgc aaggcgcgtg agctggacga gaagtatggc atcaaggatc
    15601 gcgtcgagga gggcgcgcgg gtggcgagcg acgcggcgcg gcgcggggcc gacaaggtgg
    15661 cgggcgaggc cgcgcgtgcg cgcgaagagg ccgagcgtct ggccgaagac ttcgacctgc
    15721 gcgagcgcgc cgacgaagta cgcacgcacg ccgagcgcgc ggccgaggag gcgacgcgtc
    15781 gcgcgcgcga ggc
//
LOCUS       DASVES010000029         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1715384, whole genome shotgun sequence.
ACCESSION   DASVES010000029 DASVES010000000
VERSION     DASVES010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715384"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..701)
                     /locus_tag="VF240_01210"
     CDS             complement(93..701)
                     /locus_tag="VF240_01210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7173889.1"
                     /translation="MNTRNDSNDRPRRPRDQQRFRLIPARPEHAALWRRWRGETDSQR
                     FNPLFDLSVEELARRLKVLGEGDISERRRTDYRWMVECGAEVIGTVSAMNISWGMGYV
                     EIGYMFGAEHHGRGLGTRAVALLVGKLFGETALHRVYATVSVENHASIRLLERLGFRR
                     EGVMREHYLIQGRRVDEVLFGLLRHEWEAHPFNPTTGAGESR"
     gene            complement(732..1616)
                     /gene="lpxC"
                     /locus_tag="VF240_01215"
     CDS             complement(732..1616)
                     /gene="lpxC"
                     /locus_tag="VF240_01215"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7173890.1"
                     /translation="MIKQTTIAAPVELTGMGLHTAAPVHLRLVPAPPDTGYVFVRTDL
                     DNFEIPATVEHIAHCSYATTLMRTGVMLSTVEHLLSALRGCAVDNVYVEIDNLELPIM
                     DGSADDFTHLITSTGLSAQPAMRRALRVRERVRVEQGTRSMEVEPGDGFEIDCTIDFT
                     HPLIGRQHFFFDALDAEGFAREISPARTFGFTEEIEALRRANLIRGGTLENAIVLTPD
                     GMLNETPLRTPDEFVRHKILDIIGDLALLGMPVLGRFTAVRSGHIIHAMLMSKLLRAD
                     AAWEIVDMPVAAGAAVCR"
     gene            2113..>3210
                     /locus_tag="VF240_01220"
     CDS             2113..>3210
                     /locus_tag="VF240_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX7173891.1"
                     /translation="MPSKKSTPKEPAERLFLIDSFSHIFRAFFAPMGGRSEPLTNSRG
                     QVTQAVFVFTSMLRKLLADEQPHYIAAVFESETPTFRHEMNVGYKANRVAAPEDLQTQ
                     IPYIIRVCEAYNVPILNAPGFEADDVIGALAAQATEAGLQAVVVSNDKDMCQLVSDPW
                     VICMRQNSQNVKRKEPVPPVEWCDEAWVEQKFGVPPSKIVDLLGLMGDAVDNIPGAPG
                     IGAKGAVQLVQQFGSIEEALKRWEEVKHKTYRESLRDNAPLILQSKELATIKTDVNIK
                     LDLDRLRLKPPDRPAAYALFRELEFQTLTREFADAAAQVDVGAAPRNYRQIRRAEELE
                     PLVRKLWDTDHWSFAVSDSSPAGAGQQASVHD"
BASE COUNT          582 a         1030 c         1050 g          548 t
ORIGIN      
        1 caactccgcg ccctctgcga ctcggcggtg agccgactta ctacacactc aactgatgaa
       61 ctgcaataac gcgacttgag attctttcgc cgctaccgcg actcgcccgc acccgtggtc
      121 gggttaaacg gatgcgcttc ccactcgtgt cttaacaatc cgaacaacac ttcgtccacg
      181 cgccggccct gaatcagata gtgctcgcgc atgacgccct cacgccggaa gccgaggcgt
      241 tcgagcaggc ggatggaggc gtggttctcg acgctcacgg tcgcgtacac gcggtgcagg
      301 gcagtctctc cgaacagctt tccgacgagg agcgcgacgg cgcgcgtgcc gaggccgcgc
      361 ccgtggtgct ccgcgccgaa catgtagccg atctccacgt agcccatgcc ccacgaaatg
      421 ttcatcgccg agaccgtccc gatgacctcc gcgccacact cgaccatcca gcggtagtcc
      481 gtgcgccgac gctccgagat gtcgccctcg ccgagaacct tcagacgccg cgcgagttct
      541 tcgaccgaga ggtcgaagag gggattgaac cgctgcgagt cggtctcgcc acgccagcgg
      601 cgccacaggg ccgcgtgttc agggcgtgcg gggatgagcc ggaatcgctg ttggtcgcgc
      661 gggcgtcgcg ggcggtcgtt gctgtcgttg cgggtattca agtttccagg tggggcggct
      721 ggagggcggc gtcagcggca gacggccgcg ccggcggcga cgggcatgtc cacgatctcc
      781 cacgcagcgt cggcgcgcag gagtttcgac atcaacatgg cgtggatgat gtggccgctg
      841 cggacggccg tgaagcgccc gaggacgggc atcccgagga gcgcgaggtc gccgatgatg
      901 tctaaaatct tgtggcgcac gaactcgtcc ggggtgcgca ggggcgtctc gttgagcatc
      961 ccgtcgggcg tgagcacgat ggcgttctca agcgtgccgc cgcggatgag gttggcgcgg
     1021 cgcagggcct cgatctcttc ggtgaagccg aaggtgcgcg ccggggagat ttcgcgggcg
     1081 aagccttcgg cgtcaagcgc gtcgaagaag aagtgctggc ggccgatgag cgggtgggtg
     1141 aagtcaatcg tgcagtcgat ctcgaagcca tcgcccggct cgacctccat cgagcgcgtg
     1201 ccctgctcga cacggacgcg ctcgcgcacg cgcagcgcgc gacgcatcgc gggctgcgcc
     1261 gacagccccg tgctggtgat gaggtgtgtg aagtcgtcgg ccgagccgtc catgatgggc
     1321 agctccagat tgtcgatctc gacgtagacg ttatcaaccg cgcagccgcg cagggccgag
     1381 agcaggtgct cgaccgtgga gagcatgacg cccgttcgca tgagcgtggt ggcgtaagag
     1441 cagtgggcga tgtgctcgac cgtggccggt atctcgaagt tgtcgaggtc ggtgcggacg
     1501 aagacgtagc cggtgtcggg cggcgctgga accagacgca ggtggacggg ggccgccgtg
     1561 tgcagtccca tgcccgttag ctcgacgggc gcggcgatgg tcgtctgttt aatcaatgtc
     1621 gggtcattca acttggggag gcaaggatgg cagagcgtac agatgtttgg caacgctcgt
     1681 gccgcagcgg cgggacggtg ccttgtaagt tttcccggcg gttgagagcg ccgctgaata
     1741 acatgtgtgg gcgaagcgag acagacacgc cacatcaatg ctgcgagcgc gcaacagtga
     1801 agaaaaagga tgacagccgc gttagcggcg acagaatgta gcccacggcg cgagccgtgg
     1861 gaacaggtca acaaataatc gaagccccgg aggggcggaa gaatcgcaaa ccgcgcttga
     1921 tgcgagtctg agattcttcc gcccctccgg ggcttctaat tccttaatga acttaacccc
     1981 acggctcacg ccgtgggcta cattctgtcg cccctccggg gctgcctatg tcctgtcacc
     2041 ccttgcctgt cgcccctcgc ggcttttcgc cttgtcaccc gtcgccgtcg caacttatca
     2101 tcgaagataa tcatgccgtc gaaaaaatct acgccgaaag aaccggccga gcgtctgttc
     2161 ctgatcgact cgttctcgca catcttccgc gccttcttcg cgccgatggg cgggcgctcc
     2221 gagcctctga cgaacagccg cgggcaggtg acgcaggccg tcttcgtctt tacgagcatg
     2281 ctccgcaagc tcctcgccga cgagcagccg cactacatcg ccgccgtctt cgagtcggag
     2341 acgccgacct tccgtcacga gatgaacgtc ggctacaagg ccaaccgcgt cgccgcgccc
     2401 gaagacttac agacgcagat tccctacatc atccgcgtct gcgaagccta caacgtgccc
     2461 atcctcaatg cccccggctt cgaggccgac gacgtgatcg gcgcgctcgc cgcgcaggcc
     2521 acggaggcgg gcttgcaggc cgtcgtcgtc tcgaacgaca aggacatgtg ccagctcgtc
     2581 agcgacccgt gggtcatctg catgcggcag aactcgcaga acgtcaagcg caaggagccg
     2641 gtgccgcccg tcgagtggtg cgacgaagcg tgggtcgagc agaagttcgg cgtgcccccg
     2701 tcgaagatcg tcgatctgct cggcctgatg ggcgacgcgg tggataacat tccgggtgcg
     2761 cccggcatcg gcgcgaaggg agcggtgcaa ctcgtccagc agttcggctc catcgaagag
     2821 gctttgaagc gttgggagga ggtcaagcac aagacctacc gcgaatcttt gcgcgacaac
     2881 gccccgctca tcctccagtc gaaagagctg gcgacgatca agaccgacgt gaacatcaag
     2941 ctcgacctcg accgcctgcg tctgaaaccg cccgaccgtc ccgcggccta cgcgctcttc
     3001 cgcgagctgg agtttcagac tctgacgcgc gagttcgccg acgccgccgc gcaggtcgat
     3061 gtgggcgccg ccccgcgcaa ctatcgacag attcgccggg ccgaagaact ggagccgctc
     3121 gttcgcaagc tctgggacac agaccactgg agcttcgccg tctccgattc ctccccggcc
     3181 ggcgcgggtc aacaggcttc cgtccacgac
//
LOCUS       DASVES010000030        18128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_160229, whole genome shotgun sequence.
ACCESSION   DASVES010000030 DASVES010000000
VERSION     DASVES010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18128
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160229"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="VF240_01225"
     CDS             complement(<1..1819)
                     /locus_tag="VF240_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX7173892.1"
                     /translation="MSENEKRRGLESLAAKVEAADESSAVQPSHYGPDEKELEERAGL
                     GVDEGTAGSTANGSAAESADSGSVGVEPRGHDGATEGVRAQPPEEFTGLRVGGVKTSA
                     GGVPAVVSSLKHAWREMGARRGARTLLRVNQKDGFDCPGCAWPDPDGERSHFEFCENG
                     AKAVAEEATTRRVTPEFFREWSVSDLSHKSDFWLGKQGRITEPVLLRRGATHYESVSW
                     DEAFRVVAGELNALASPDEAAFYTSGRASNEAAFLYQLFVRQFGTNNLPDCSNMCHES
                     SGRGLTETIGVGKGTVTLEDFQLAEVIFIIGQNPGTNHPRMLTSLQEAKRRGCKIVHI
                     NPLPETGLQRFKHPQNPLDLLGRGTQLSDLFLQVRINGDVALLKGVMKEVLEEESRRP
                     GQVLDREFIEEKSAGFEEFARALEGVGWDDIVEQSGVSRESIREAARIYVESSATIVC
                     WAMGLTQHKNAVANIQEIVNLLLLRGQIARPGAGPCPVRGHSNVQGDRTMGIWESPSA
                     SFLDALAREFDFEPPRRHGLDTVETIKAMHGGRVKVFFALGGNFLSATPDTEYTARAL
                     GRLRLTAHVSTKLNRAHLVTGEQALILPCLGRTETDVQKS"
     gene            complement(2116..3522)
                     /locus_tag="VF240_01230"
     CDS             complement(2116..3522)
                     /locus_tag="VF240_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173893.1"
                     /translation="MNNVTRMKLLAVVVFAAALSASLVAGAQNRQPQRPREEPRASSF
                     SPVVEEPFDVVLARDKANKPRVMAAARKLLEDRYDLARRVDPVVKMTRGKPIPVGPTA
                     RLARGVTWEQLGAMSPEEVREKNLFPYLPLPHVSHPVGGMVFPQVHVKAQPRLERFDM
                     DFDIPDHFLPEFPPAIFLTTRPDLGDVSKGQLVTINNFYDLFNGILNPKQLDGLRLLV
                     SQFPQQQFNATADRKTERNDGMQGVTCLDCHVNGHTLGATHLVGDIRPQENRRRIETP
                     SLRGVNIQRLFGSQRALRTVEDFTEFEQRAAYFDGDPVTAAKKGVNTLDRALHVHAMA
                     EFQELLDFPPAPKLNVYGRLDRRKATESEARGEDLFFGKANCAVCHTPPYYTDGTMHD
                     LQVERFYRPRMEGGMMITKQGPIKTFPLRGIKESPPYLHDGRLLTLEDTVEFFSLVLQ
                     LNLTAQEKSDLVAFMRVL"
     gene            3777..4238
                     /locus_tag="VF240_01235"
     CDS             3777..4238
                     /locus_tag="VF240_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7173894.1"
                     /translation="MDVFQILKEDHKKVSGIFAKLEETTENAAKTREELFTKLKTELD
                     IHAHVEETQFYPVIKREAETREITLEGFEEHHVIKMLLKELEAGDVGSEQWTAKLKVL
                     KENVEHHVEEEEDEMFVKAREVLSAEQIESITANVEAEKKAQLQKAAAAGQ"
     gene            4387..5169
                     /gene="modA"
                     /locus_tag="VF240_01240"
     CDS             4387..5169
                     /gene="modA"
                     /locus_tag="VF240_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7173895.1"
                     /translation="MALLLLLLTFCGCQAATPDPATDETRDTGNLTVSAAVSLKDAFS
                     ETGNLYKARTGKTVNFNFGASGALQRQIEVGAPVDVFASAGTQQMDALAGRGLLDEAS
                     RRDFARNTLVLIVPPDSPLNLTAFADLADARVQKIAVGNPKTVPAGQYTEQVFNHVNL
                     RDKLQAKLILAEDVRQVLDYVVRGEVEAGVVYATDARAAGDRVRVVATAAEDMHAPIL
                     YPIAVIKQSTQKLTAQEFVNLVAGAEGQSILRKYGFASAGER"
     gene            5166..5828
                     /gene="modB"
                     /locus_tag="VF240_01245"
     CDS             5166..5828
                     /gene="modB"
                     /locus_tag="VF240_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX7173896.1"
                     /translation="MIWSSLKLSLLVVSIATLIVGALGLAFAFLLAKRNFRGKELLDA
                     VLTMPLVLPPTVTGYYLIVLLGRRGLLGSLIYDLTGWTLVFTWQGAVIAAVVVALPLM
                     IKAARASIESVNPQYEIASYTLGKSELETFFRITLPLARRGVLAGLILSFARALGEFG
                     ATLMIAGNIPGKTQTMPLAIYEAVASGDDRRAQILAVVLTAISILAVYLTNKISRSQV
                     YG"
     gene            5832..6509
                     /locus_tag="VF240_01250"
     CDS             5832..6509
                     /locus_tag="VF240_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7173897.1"
                     /translation="MLDADIKKRFGDRRHPAFLLDVRFFVRNGITALVGASGSGKTTC
                     LRLIAGVLTPDEGRISVGEQTYFDSSRKINLSIQKRRIGFVFQDYALFPHLTAEQNIA
                     YGVKADDRKTKYDKAREMLSLFHIEHTRRRFPGEMSGGEGQRVALARALASDPAVVLL
                     DEPLSAVDVETRTKLLDEIEDAQRKANIPFIYVTHNEAEAARLGTHRITLHGGRIAHD
                     KAFEETA"
     gene            complement(6572..7801)
                     /locus_tag="VF240_01255"
     CDS             complement(6572..7801)
                     /locus_tag="VF240_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7173898.1"
                     /translation="MPEAATENSEGPSRWRALALLALAELLAMSLWFSASAVVPAIRA
                     EWGLTDSQAGWLTLSVQLGFVAGTLISAVFNLSDVMNTRRLLALSAVGGAIVNAAFAL
                     STHELASGLALRFLTGLFLAGVYPPGMKIMATWFRRGRGMALGVLVGALTLGKAAPYL
                     VNAVGSAEWRVNVVVASALAVVGALVVLLFVGDGPLALAPARFRLGQAARVFRNRGVR
                     LANFGYFGHMWELYAMWTWAPVMIRAGLAERGADASLAEVASFLVIGAGAAGCVAAGL
                     LADRVGRTLVTSWAMGISGACCLLVGFVYDAHPALLVAVAVVWGASVVADSAQFSACV
                     TELGDPRYMGTALTIQTCCGFLLTTVSIQLIPVLVERVGWRYAFAALAPGPLFGVVSM
                     LRLRRLPEAAQIAHGKR"
     gene            complement(8150..8542)
                     /locus_tag="VF240_01260"
     CDS             complement(8150..8542)
                     /locus_tag="VF240_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7173899.1"
                     /translation="MALLIGDDGDTVMVVDDSDDIREIICVRLRRMGYRVVEAANGEE
                     AVKLTTRTHPDLILMDLSMPVMDGYEATRRIKAMPGCGGIPVVAVSAFCDSRSRHEAL
                     EAGCVEYVSKPVDFGKIDGLVSRHLQTH"
     gene            8871..10385
                     /locus_tag="VF240_01265"
     CDS             8871..10385
                     /locus_tag="VF240_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7173900.1"
                     /translation="MFCRVDGTRLITDSLTSDSTATAILHSAPVSGRTSTRSLQGTPS
                     IAILPFANMSNDAEYEYFCDGLAEELLNALAKVENLKVAARTSAFSFKGSNTNVSEIG
                     RVLNVNSVLEGSVRKSGDKLRITVQLINASDGYHLWSERYDRQMKDIFDVQDEITMAV
                     VDALRVKLLGEDKAALLKRYTDNTEAYELYLKGRYFYNKYTAEDLQKGIEYFERAIKK
                     EPEYAPAYAGIGFCYGALWYFGFFAPHEIMPKWRAVISRALEIDSDLADAHLSLASIH
                     FYYEWNFTKAERSYQRAIVLNANSPDAHWRYGHFLAALERFEEAISEGRQALELDPLS
                     LVTKFFMVRIYWFANRLDDALEQAQGMIEMEPSFPSAYVALGGVCLQKGLYEEAVKAY
                     QQALTLHGFVGSALSYLGAVYGIMGKRDEAHNVLSQLLEMRKTQHVTAYRIACVYGGL
                     GENDKAFEWLEKAVEEKNGEIVFLKREVEANIFDESIRQDPRFADLMRQVGLPQ"
     gene            10582..11691
                     /locus_tag="VF240_01270"
     CDS             10582..11691
                     /locus_tag="VF240_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173901.1"
                     /translation="MPHLKTPLRTFAGLSLLMGLSLILSSGCATSGGTPKPDGVFPEA
                     VGKYKLVNVEDGRDAEYENEKNKGQRYQSWRAKYSDGANDIFYQVGSHKSAEEAANEL
                     GFLTRCFDSNRKLMSNNKIWDGIPLKDGSGKAVGAMAVCFSDKSETNRSYGNGGLGDY
                     EYAFGLSNDNRTYTVKPLKENTALVAEFVKALPFNSGVDLSALDAFARAKAAKPPTYK
                     EMFDTLPPVKLAAKPYLKGKVLVLERDTTELEQSTSVGNPTNYYVTETNRQAMLPSDI
                     GSIVRVDCRRSRKVGTYTAGAGDALPAYGATCEVLVIDNTVPAVIARKTFANEDMADV
                     QLVDTDKKGKVTLKEYILPKPISEIKDFVQALPAN"
     gene            12093..13358
                     /gene="eno"
                     /locus_tag="VF240_01275"
                     /pseudo
     CDS             12093..13358
                     /gene="eno"
                     /locus_tag="VF240_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            13491..15044
                     /gene="gpmI"
                     /locus_tag="VF240_01280"
     CDS             13491..15044
                     /gene="gpmI"
                     /locus_tag="VF240_01280"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX7173902.1"
                     /translation="MTDRRGPLALIILDGFGHSDVREGNAVALARMPFYDELLEKYPH
                     TLIEASGECVGLPAGVMGNSNVGHLCMGAGRVVRTDIERINHEVSSGAFDSNLALESA
                     VESALKHDRALHLIGLTSDGLVHSSQEHAYALLRLAERRGLRRVYVHCFLDGRDTPPS
                     SADRYVRQMQEKCAEIGCGEVASVVGRYYAMDRDRRWERTERAYRLLVHGEGARASDP
                     VAAIKSSYERAVTDEFVEPVIVTRGEGGEPVATIAAGDSVIFFNFRADRARQITSALA
                     VPGFDDFAVPNRPHTHFVCFAVYDKTYPLPVAFPPTQPTNILAEVFAAAGVRNYRMSE
                     TEKYAHVTYFFNGGNEREYPHERRLLVPSPKVATYDLAPEMSAFKVTDKVLRAIEEGD
                     TDVFILNFANPDMVGHTGNLSKTIEAVQHVDTCLGWITKALKTARGRAIITADHGNCE
                     QMIDHTTGQPHTAHTSNPVPFVLVDHDSRGARLRDDGGLEDVAPTMLGLLGLEKPAEM
                     TGRDLRLTD"
     gene            15121..16179
                     /locus_tag="VF240_01285"
     CDS             15121..16179
                     /locus_tag="VF240_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7173903.1"
                     /translation="MNSDPRKILVIDFGQLGDVVLSLPALAAVRGRFPRARVTVAVGK
                     PGVPVVEMSGYADEVMGVDRVALRDGPKPMSILRIVQFVKDVRRERYDLVIDLHSLSE
                     TNLLGFFSGASQRLFARRPGRSLDFLSNFHTRPPVEDAKKHAIDRYLDVLRPLDVGEV
                     SRVPRLRTRAEDHAAVDEFLKQELPRKERARSSNSSDDAPLVGLFPGAGHPSRRWPLE
                     RFAELARRLEGNDSVRALLFAGPEERRVVREARAQFPRSTILLDRLSVPQLAAAQERL
                     AVFVSNDTGPMHIAAAVGTPVVILMQHHAAFECYIPPGERHRVVHAPTIEEITTDAAY
                     TATRAAFTAERTASLFAS"
     gene            16186..17850
                     /gene="dacB"
                     /locus_tag="VF240_01290"
     CDS             16186..17850
                     /gene="dacB"
                     /locus_tag="VF240_01290"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX7173904.1"
                     /translation="MGATHQSRRTRRARGAAVAALFLLTTFTSVACRDSSSSTGANEG
                     AALPAESAAGEDAARGGRAARTAEETRKGDAALTAELDRQIDERGPAAARWGISVVSL
                     RDGRVLYERDAGRLFTPASVMKVYTTAVALDTLGADYRWRTSVYAVSAPTAGGTIEGD
                     LVLYGRGAPDLTSRDTRAGPSQLDELAEQLRRRGVRRVRGDVVGDESYFRGETLGEGW
                     LWQDVQWYYGAEPSALTVDGNEITVSVAPGANTDDPVEVKTVPPTDYLRVTNDMRTVE
                     RGGRPRIGITRGLSSNDVRVWGEFPAGGPPYGVRLSVHRPALLAARLLRAALRSRGIE
                     VAGEARSVDARVPEDERLDSDRAVELAHVLSRTLGEVVRETNKESVNLDAELILRAVG
                     RERGDTAPAADERRTRQRDTVVAGLAVVRRWLEGAGLDTEPLALHDGSGLSRFDLVTP
                     EATARLLAHISKTPSAAVFRDSLPVSGRDGTLQGRLRQADPPIEAKTGTLVYVNGLAG
                     YATTADGERLAFAIFCNDKTESQSSLRAIDAVALTLTRYPRPAELR"
BASE COUNT         3461 a         5816 c         5664 g         3187 t
ORIGIN      
        1 ccgacttctg cacgtcagtc tccgtccggc ccagacacgg caggatgagc gcctgctcgc
       61 cagtgacgag atgcgcgcgg ttgagcttcg tcgagacgtg cgcggtgagg cgcaagcgcc
      121 ccagtgcgcg tgcggtgtac tcggtgtcgg gcgtggccga aagaaagttg ccgccgaggg
      181 cgaagaagac tttgacgcgg cccccgtgca tcgccttaat ggtctcgacg gtgtcgaggc
      241 cgtgccggcg cggcggctcg aagtcgaact cgcgtgcgag cgcgtcgagg aagctggccg
      301 aggggctttc ccagatgccc atcgtgcggt cgccctgcac gttcgagtgg ccgcgcacgg
      361 ggcacgggcc ggcgccgggc cgggcgatct gcccgcgcag cagcagcagg ttgacgatct
      421 cctgaatgtt ggcgacggcg ttcttgtgct gcgtcagccc catcgcccag cagacgattg
      481 ttgcgctcga ttcgacgtag atgcgcgccg cctcccgtat cgactcgcgc gacacgccgc
      541 tctgttcgac gatgtcgtcc caaccgacac cttcgagcgc gcgcgcaaac tcctcgaacc
      601 ccgccgactt ctcctcgatg aactcacggt cgagcacctg tccggggcgg cgcgactctt
      661 cttccagcac ttccttcatc acacctttga ggagcgccac gtcgccgttg atgcggactt
      721 gcaggaagag gtcggagagc tgcgtgccgc ggcccaaaag gtcgagcgga ttctgcgggt
      781 gcttgaagcg ttggagtccg gtctcgggca gcgggttgat gtgaacgatt ttgcagccgc
      841 gccgcttcgc ctcctgaagc gaggtgagca tgcgcgggtg gtttgtcccc ggattctggc
      901 cgatgatgaa gataacttcg gcgagttgga aatcttcgag cgtgacggtg cccttgccga
      961 cgccgatggt ctcggtcagg ccccggcccg acgactcgtg gcacatgttc gagcagtcgg
     1021 gcaggttgtt ggtgccgaac tgccggacga agagttgata aaggaaggcc gcctcgttcg
     1081 acgcgcgccc cgaagtgtag aaggccgcct cgtccggtga cgcgagcgcg ttcaactcgc
     1141 cggcgacgac gcggaaagcc tcgtcccaac ttacggactc gtagtgcgtc gcgccgcgcc
     1201 gcagcagcac cggctcggtg atgcggccct gcttgccgag ccagaagtcg gacttgtgag
     1261 agaggtcaga gactgaccac tcgcggaaga actcgggcgt gacgcgcctc gtcgtcgcct
     1321 cctcggcgac ggccttcgcc ccgttttcac aaaactcgaa gtgagagcgc tcgccgtcgg
     1381 ggtcgggcca cgcgcagccg gggcagtcga agccgtcctt ctggttgacc cggagcagcg
     1441 tgcgcgcgcc gcgccgcgcg cccatctcgc gccacgcgtg cttgaggctg gagacgaccg
     1501 ccggcacgcc gcccgcgctt gtctttacgc ccccgactcg cagccccgtg aattcctcgg
     1561 gcggctgcgc gcgcacgccc tccgtcgcgc cgtcgtgacc gcgcggttcg acaccaacgc
     1621 tgccgctatc cgcggactcc gcggcggagc cgttggccgt cgagccggca gtcccttcgt
     1681 ccacaccaag cccggcccgc tcttcgagtt ccttctcgtc cggcccgtag tgcgacggct
     1741 gcacggccga agactcgtcc gccgcctcga ccttcgcggc cagcgactca aggccccggc
     1801 gcttttcatt ctcactcatt accagaggag tttagtgcaa gccgtgtcgg ttcgcacgcg
     1861 cggctccgta gcaggcaacg agccgcggca gcggcggcag agtgtagccc acggcgtaag
     1921 ccgtggggat aagttcgatt aaggaaccgg aagccccgga ggggcgaaag aatttcaacc
     1981 ggcgtgaaac gcaaggtaga tattctttcg cccctccggg gcttgttttt gcttccgctg
     2041 ccggttccac gggcttacgc ccgtggctac attctgtcgc cgcttacagc ggctcgttga
     2101 cgcgtgcgac ccgcgtcaga ggacgcgcat gaaggcgacg aggtcagact tctcctgcgc
     2161 cgtcaggttg agttggagga cgaggctgaa gaactcgacg gtgtcttcga gcgtgagcag
     2221 gcggccgtcg tggagatagg gcggcgactc cttgatgccg cgcaggggga aggtcttgat
     2281 cggcccctgc ttggtaatca tcatgccgcc ttccatccgg ggacggtaga agcgttcgac
     2341 ctgaaggtcg tgcatcgtgc cgtccgtgta gtaaggcggc gtgtggcaga cggcgcagtt
     2401 ggccttgccg aagaagaggt cttcgccgcg cgcctccgat tcggtcgcct tccggcggtc
     2461 gagtcggccg tagacgttga gctttggcgc gggcgggaag tcgagaagtt cctgaaactc
     2521 ggccatcgcg tgaacgtgga gcgcacggtc gagagtgttg acgcccttct tggcggccgt
     2581 gacggggtcg ccgtcgaagt aggcggcgcg ctgctcgaac tcggtgaagt cttccacggt
     2641 acgcagcgcc ctctgcgagc cgaagagacg ctggatgttg acgccgcgca aactgggcgt
     2701 ctcgatgcgt cggcggttct cctgcgggcg gatgtcgccg acgaggtgcg tggcgccgag
     2761 cgtgtggccg ttgacgtggc agtcgagaca ggtgacgccc tgcatgccgt cgttgcgctc
     2821 ggtcttgcgg tcggccgtgg cgttgaactg ctgctgcggg aactgcgaga cgagcaggcg
     2881 caggccgtcg agctgcttcg ggttgaggat gccgttgaag aggtcgtaga aattgttgat
     2941 ggtgacgagc tggcccttcg acacgtcgcc gaggtcgggc cgcgtcgtca ggaagatggc
     3001 gggcggaaac tcggggagga agtggtcggg gatgtcgaag tccatgtcga agcgctctaa
     3061 ccggggctgc gccttgacgt gtacctgcgg gaagaccatg ccgccgacgg ggtgactgac
     3121 gtgcggcagc ggcaggtacg ggaacaggtt cttttccctg acctcctcgg gcgacatcgc
     3181 gcccagttgc tcccacgtca cgccacgcgc gagccgcgcc gtcggcccca cggggatggg
     3241 cttgccgcgc gtcatcttga ctacgggatc gacgcgccgc gcgaggtcgt agcgatcctc
     3301 aaggagcttg cgcgcggcgg ccatgacgcg cggcttgttc gccttgtcgc gcgccagaac
     3361 cacgtcgaac ggctcctcaa ccaccggcga gaaggaagac gcgcgcggct cctccctcgg
     3421 cctctgcggc tggcggttct gcgcgcccgc gacgagcgac gccgacaacg cggcggcgaa
     3481 gacgacgacg gccaaaagct tcatgcgtgt gacgttgttc atgctccctc ctccgtttcg
     3541 ggctatgggc cggccggtga aagaacttga ctgctcaggt tgggggtatg actggcgaag
     3601 gtgtacggct gcggacataa tacgccggcc gaacgatttt gcaatgaaaa tgtgaggccg
     3661 ccggccgtgc ccatcggagc cgctggcact gcgtttgact actctactgc aagcgccgac
     3721 ttcacgtact gtaacagtgc aagcagggtg tttataaacc ataacggagg actgacatgg
     3781 acgtatttca aattctgaag gaagaccata agaaggtttc gggcatcttc gcgaagcttg
     3841 aagagacgac ggagaacgcc gccaagacgc gcgaggagct gttcacgaag ctcaagacgg
     3901 aactcgacat ccacgcgcac gtcgaggaga cgcagttcta ccccgtcatc aagcgcgagg
     3961 ccgagacgcg cgagattacg ctcgaaggct tcgaggagca ccacgtcatc aagatgctcc
     4021 tgaaggagct ggaggcgggc gacgtgggga gcgagcagtg gacggccaag ctcaaggtgc
     4081 tcaaggagaa cgtcgagcac cacgtcgaag aggaagagga cgagatgttc gtcaaggcgc
     4141 gcgaggtgtt gagcgccgag cagatcgaga gcattacggc caacgtcgag gccgagaaaa
     4201 aggcccagct gcaaaaggcc gccgccgccg gtcagtagcc ctaagacatc agatcaatga
     4261 acgggccgcg tgtcgctcct ctgtgcgttg aaattcgcac tggggagcga cgcgcggcct
     4321 gttttttttc gatgacgctt atcggatagc tcacctgcac aagcatgtat aatgccgccc
     4381 atgcgattgg cgttgcttct gctcttactc acattttgcg gctgccaagc cgcaacgcct
     4441 gatcccgcaa ccgatgaaac acgcgacacc ggcaatctta cggtgtctgc cgccgtgtcc
     4501 ctcaaggatg cttttagtga aacaggaaat ctgtacaagg cgcggacggg gaaaaccgtc
     4561 aacttcaact tcggcgcgtc gggcgcgttg cagagacaga tcgaggtggg agcgccggtc
     4621 gatgtatttg ccagcgcggg cacgcagcag atggacgcac tcgcggggcg cgggttgctt
     4681 gacgaagcga gccgccgcga cttcgcgcga aacacgctgg ttctcatcgt cccgccagac
     4741 tcgccactga atctcaccgc gtttgctgac cttgccgacg cccgcgtgca aaaaatagct
     4801 gtcggcaatc cgaaaaccgt tccggcggga cagtacacgg agcaagtttt taaccacgtc
     4861 aacctgcgcg acaagttgca ggcgaaactc atcctggccg aagatgtgcg acaagtgctt
     4921 gattacgttg tgcgcggtga agtggaggcc ggcgtcgtgt atgcgacgga cgcgcgcgcg
     4981 gcgggtgatc gtgtgcgcgt cgtcgccacg gcggcggaag acatgcacgc gccgattctg
     5041 tatccgattg cggtcatcaa acagagcaca caaaagctga cggcgcaaga gttcgtcaat
     5101 cttgtcgccg gcgcggaagg gcagagcatc ctgcgtaaat atggttttgc gagcgcgggc
     5161 gaaagatgat ctggtcgtcg cttaaactct cgttactcgt tgtcagtatc gccactttaa
     5221 tcgtcggtgc gctcggtctc gccttcgcct tcctgctcgc caagcgcaac tttcgcggta
     5281 aagaactgct cgacgcggtg ctgacgatgc cgctcgtcct gccgccgacg gtcacgggtt
     5341 attacctgat cgtgctctta ggccggcgcg ggctgctcgg cagcttgatc tacgatctca
     5401 cgggctggac gctcgtcttc acctggcaag gcgcggtgat cgccgccgtt gtcgtcgcgc
     5461 tgccgttgat gattaaagcg gcgcgggcga gcatcgaaag cgtcaacccg caatatgaga
     5521 tcgcctccta cacgctcggc aaaagcgaac tcgaaacctt cttccgcatc acgttgccgc
     5581 tcgccagacg cggcgtcctg gccggactta ttttgagctt cgcgcgtgcg ctcggtgagt
     5641 tcggcgcaac tctgatgatc gcggggaaca tcccgggcaa aactcagacg atgccgctgg
     5701 ccatctacga agccgtggcc tcgggcgacg accggcgggc gcaaattttg gcggtcgtgc
     5761 tcacagcgat ttccatcctc gccgtctacc tcaccaacaa aatcagccgc tcgcaagttt
     5821 atggatagac gatgctcgat gcggacatca aaaagcgttt cggcgatagg cggcatccgg
     5881 cgttcttgct ggatgtcagg ttctttgtgc gtaatggcat cacggcgctc gtcggcgcgt
     5941 cggggtcagg caaaacaaca tgcctgcgcc tgatcgccgg agtcctgacg ccggacgaag
     6001 gccgtatcag cgttggcgaa caaacctact ttgattcaag tcgcaagata aatctttcga
     6061 ttcaaaagcg acgcatcggc tttgtgttcc aggattatgc gttgtttccc catctcaccg
     6121 ccgaacagaa catcgcttac ggcgtgaaag ctgacgaccg gaaaaccaaa tacgacaagg
     6181 cgcgcgagat gctctcgctc tttcacatcg aacacacgcg ccgacgtttt cccggtgaaa
     6241 tgtcgggcgg cgaagggcag cgcgtggcac tggcgagggc gttagcctct gacccggcag
     6301 tcgtgctgct cgacgaaccg ttatcggcgg ttgatgtaga aacgcggaca aaattgctcg
     6361 atgaaatcga agatgctcaa cgcaaagcga acattccttt catctatgtg acacacaacg
     6421 aagcggaggc cgcacgactc ggcacgcatc gcattacact acacggcggg cgcatcgcgc
     6481 acgataaagc ctttgaggaa acagcctgac caattcaatt aaagactatg accaatggat
     6541 cactgaaccg atgaaccgat gaatcaattc ttcaacgctt tccgtgcgcg atctgcgccg
     6601 cctctggcag acggcgcagg cgcagcatgg agacgacgcc gaagagcggc cccggcgcga
     6661 gcgccgcgaa ggcgtagcgc cagcccacac gctcgacgag gacggggatg agttgaatcg
     6721 acacggtcgt cagcaggaag ccgcagcagg tctggatggt gagggcggtg cccatgtagc
     6781 gcgggtcgcc gagttccgtg acgcacgccg agaactgcgc cgagtcggcg acgacgctcg
     6841 cgccccacac caccgcgacc gcgacgagca gcgccggatg cgcgtcgtag acgaagccca
     6901 ccagaaggca gcacgcgccg cttatgccca tcgcccacga ggtgacgagc gtgcggccca
     6961 cgcggtcggc gagcagtccc gccgcgacgc agcccgcggc gcccgcgccg atgacgagaa
     7021 acgaggcgac ctccgcaagc gacgcgtccg cgccgcgctc cgcgaggccc gcgcgaatca
     7081 tcacgggcgc ccacgtccac atcgcataca gctcccacat gtgtccgaag tagccgaagt
     7141 tggcgagacg cacgccgcgg ttgcggaaga cgcgcgcggc ctgccccaga cggaagcgcg
     7201 ccggcgcgag cgccagcggg ccgtcgccga cgaacagcag cacgacgagc gcgcccacaa
     7261 ccgccagcgc cgaggcgacg acgacgttca cgcgccactc cgcgctcccc acggcgttga
     7321 cgagataggg cgcggccttc cccagcgtga gcgcgccgac gagcacgccg agcgccatcc
     7381 cgcgcccgcg ccggaaccac gtcgccatga tcttcatccc cggcgggtac acccccgcga
     7441 ggaagaggcc ggtgagaaac ctgagcgcga ggcccgacgc gagttcgtgc gtcgagagcg
     7501 cgaaggcggc gttgacgatt gcgccgccga cggccgaaag cgcgagcagc ctccgtgtgt
     7561 tcatcacgtc cgagaggttg aagacggcgc tgatgagcgt gcccgcgacg aagccgagct
     7621 gcaccgagag cgtgagccac ccggcctgag agtcagtcaa gccccactcg gcgcgaatcg
     7681 ccggcacgac cgccgaggcg gagaaccaca gcgacattgc gagcagttcg gccagcgcga
     7741 ggagcgcgag cgcacgccag cgggatgggc cttccgagtt ttctgttgcg gcttccggca
     7801 tcgaaatttg gggcggggtg gttttaccct ccgccgcccc catacggcat ctgataggct
     7861 gtcagtacac gtctagtgta cttcagaacg gcaactcgta attctccaag tctacgtccg
     7921 gcgcgggatt ttccgggccg ggaaaaacta aaggcggcgc tgagaacgcc gccctgcaat
     7981 gcctttattt ggttcagaat cagcctgcaa tcgtcttttc ctaccatctg acgcaacgta
     8041 caggtacttg ctcagagttt gattttgacc ccgccgttaa agactcaccc ctccatatgc
     8101 gtgtaaatct cgctgaaggt tgggttctga tgcggcggaa aaacgaccac taatgggtct
     8161 gcaaatgcct cgacaccaag ccatcaatct tgccgaagtc caccggctta ctcacgtact
     8221 cgacgcagcc tgcctccagc gcctcgtgcc tgctacggga gtcgcagaag gccgataccg
     8281 ccacgacggg tataccacca cagccgggca tggcttttat cctgcgggtc gcctcgtagc
     8341 cgtccatcac gggcatgctc aagtccatca gaatcaggtc ggggtgggtt cgcgtggtga
     8401 gcttcaccgc ctcctcgccg ttagcggcct cgactaccct ataacccatc ctccgcagtc
     8461 gcacgcagat gatttcgcgg atgtcgtccg agtcgtccac gaccatcacc gtgtcgccgt
     8521 cgtcgccgat gagcagagcc atcaccacct ccttgatgtc ggagccatga actgcggggg
     8581 aatcttgagt ggagtctacc cactggactt gagtaattct tgaggtacgg gcgcggggcc
     8641 gttgaggcac ggttgcgagt cggttgcgat tctattacgc cgggaagtta acgacgacgc
     8701 atccccatcc cggaacgtcg ttaacacgtt ccctgccgca gattacgcta gcggccgcag
     8761 ccgtgcgcgg ttagaatgtg cgggctattc gcatctgtcc tgttagcccg gtttccgctc
     8821 cgatgaaacg ctgcccgaag tgcaaccgcg tcgagactga aaacgcgcta atgttctgcc
     8881 gcgtggacgg cactcgcctc atcaccgact ctttaacttc ggattccacg gctactgcga
     8941 ttcttcactc tgctcccgta agcggcagaa cctcgacgcg ctcccttcag ggcacgccct
     9001 caatcgccat cctgccgttc gccaatatga gcaacgacgc ggagtacgag tacttctgcg
     9061 acgggctggc ggaagaactg ttgaacgctt tggcgaaggt tgaaaacttg aaggtcgcgg
     9121 cgcggacttc tgcgttctcg ttcaaaggca gcaacacgaa cgtcagtgaa atcgggagag
     9181 tcttgaacgt caactccgtg ttggaaggga gcgttaggaa gtcaggggac aaattaagaa
     9241 tcacggttca gcttatcaac gcttcggacg gctatcactt atggtcagaa cgctacgacc
     9301 ggcagatgaa agacatcttc gacgtgcagg atgaaatcac tatggcggtg gttgacgcac
     9361 tgagagtgaa actgcttggc gaggacaaag cggcgctgct gaagcgttac accgacaata
     9421 cagaggctta tgagctatac ctcaaaggac gctacttcta caacaaatac acggccgaag
     9481 atttacaaaa aggtattgaa tacttcgagc gagcgattaa gaaagagccg gaatatgccc
     9541 ctgcttacgc gggcatcggc ttctgctacg gcgcactttg gtattttggc tttttcgccc
     9601 ctcacgaaat aatgcctaaa tggagagccg taatcagcag ggcgctcgaa atcgatagcg
     9661 acttagctga cgctcatctc tcactggcga gcattcattt ttattacgaa tggaatttta
     9721 ccaaagctga acgtagttat cagcgcgcca ttgtgcttaa tgctaacagc cctgatgctc
     9781 actggcgtta tggacacttt ttagccgctc ttgagcgttt cgaggaagca atcagcgagg
     9841 gcagacaggc gttagagtta gacccgcttt cacttgtcac gaagttcttt atggtgcgca
     9901 tctactggtt tgccaatcgc ttagatgacg cgcttgagca agcgcagggg atgattgaga
     9961 tggaaccgag tttccccagt gcgtacgtag cgctgggggg cgtgtgtttg caaaaagggt
    10021 tgtatgaaga agccgtcaaa gcatatcagc aagcacttac tttgcacggc ttcgtcggct
    10081 ccgccctgtc gtatttaggt gccgtttacg gcattatggg caaacgagac gaggcacata
    10141 acgtgctcag tcaactgctc gaaatgagga agactcaaca cgtcacggca tatagaatcg
    10201 cgtgtgttta cggtgggcta ggcgagaatg acaaagcgtt tgagtggttg gagaaagccg
    10261 tcgaagagaa aaacggcgag atagtttttc tgaaaagaga ggtcgaggca aatatctttg
    10321 acgaaagtat tcgccaagac ccgcgcttcg ccgacctcat gcgccaagta ggactcccgc
    10381 aataagcccg gagtgagagc cgcccgcgag gtgcgaacgg cttgattttt ttcttcccgg
    10441 cgtgagagcg cttgggctat actgcgcccg cctggcagcc gctggttcgt tcccacatct
    10501 tacgcgtgcc tttagttttt tctcagagct ttgcctgaca atctgaacat gaaacgcgct
    10561 acgtttcaga gaggctcaac catgccccac ctgaagactc cgctccgcac cttcgccggc
    10621 ttgtcgctgt tgatgggcct gagtttaatt ctgtcgtccg ggtgtgccac gtcgggcgga
    10681 acgccgaagc cggacggggt gttccccgaa gcggtcggca agtacaagct ggtgaacgtc
    10741 gaagacgggc gggacgccga gtatgagaac gagaaaaata agggccaacg ctatcagtcc
    10801 tggagggcga agtattccga tggggcgaac gatatctttt atcaggtcgg cagccataaa
    10861 tccgcggagg aggcagcgaa cgagctgggg tttttgaccc gctgcttcga cagcaaccgg
    10921 aagctcatgt ctaacaataa gatttgggat gggattccgc tgaaggacgg ctcgggcaag
    10981 gccgtcgggg cgatggcggt gtgcttttcg gacaagtccg agacgaacag gtcatacggc
    11041 aacggggggc ttggtgacta cgagtacgct ttcggcctca gcaacgataa ccggacgtat
    11101 acggtgaagc cgctcaagga aaacaccgcg ctcgtggcgg agttcgtcaa agcgttgccg
    11161 ttcaactcgg gtgtggactt gtcggcgctc gacgcattcg cgagggcgaa ggccgcgaag
    11221 ccgcccacct acaaggagat gttcgacacc ctgccgcccg tgaagttagc cgccaagcca
    11281 tacctgaagg ggaaagtgct ggtccttgag cgggacacca ccgaattaga gcaatccacc
    11341 tcggtgggga accccacgaa ctactacgtc acggagacca accggcaagc gatgttgccg
    11401 agcgacatcg gctcaatcgt gcgggtggat tgtcggagaa gcaggaaggt gggcacttat
    11461 actgccggtg cgggggacgc gcttcccgcc tacggggcga cgtgtgaagt cctcgtcata
    11521 gacaacaccg tgcccgcggt gatcgccagg aaaaccttcg cgaacgagga catggccgac
    11581 gttcagttgg tagacaccga caagaagggc aaggtgacac tcaaagagta catactaccc
    11641 aaacccatca gcgagattaa ggacttcgtt caagccctcc cagccaacta acgcggaagg
    11701 tacgtgaacc gcccgacacg ccgagaatgt ggcaaggtca ctctcaatct cgcgtttccc
    11761 ttttgccgac ttgtgtactg cttctacact tcgcccccaa tgaggggagt cagccgactt
    11821 gaaatgaaga actccgaccc tgaatacctc agttaactcg cccattcagc aagcctttgc
    11881 atccccggcg gatacatccc cgcgaggaac agtcccgtca aaaatctgag cacgatgtcg
    11941 gaagttaatt cttgcgaagt taacgcgaag ggggcgttga cggctgcgcc gccgactgcc
    12001 gaccgtcctc gcgctttttg actgtgctcc gccgggttga tagagtttgc ggtgattcag
    12061 caatcaaatt attgaggagc gcgctcaaaa ctatgagctt gatcgattcc gtgtccgcgc
    12121 gcgagatttt ggactcgcgc ggcaacccga ccgtcgaggc cgaggtctct ctggagagcg
    12181 gcgaggtcgg ccgcgcggcc gtcccctcgg gcgcctccac cggcgagcac gaggccgtcg
    12241 agctgcgcga cggcgacaag aagcgttacc tcggccgcgg cgccctgcgc gccgtcgcca
    12301 acgtcaacga gtcaatcgcg ccggaactcg aaggcatgga cgcgctcgac caggccgaag
    12361 tagacgccgc gctcctcgcg ctcgacggca cgcgtaacaa gtcgaaactc ggcgccaacg
    12421 ccctgctcgc cgtctcgctc gccacggcgc gcgcgggcgc ggcctacctc gaagtcccgc
    12481 tctaccgcta cctcggcggc cccaacgcgc gcacgctccc cgtcccgttg atgaacatcc
    12541 tgaatggcgg cgcgcacgcc gacaacaacg tggactttca ggagttcatg gtcgtcccca
    12601 cgggcgccga aagttttcgc cacgcgcttc aggtcggcgc cgaaatcttc cacgccctga
    12661 aggccgtgct caagaaacga ggactggcga cgagcgtcgg cgacgagggc ggcttcgcgc
    12721 ccaacctgcg ctcgaacgaa gaggccgtcg agatcatcct cgaagcgacg gccgcggccg
    12781 gctacaacgc cgggcgcgat gtgtacctcg cgctcgaccc ggcggcgagc gagttctatg
    12841 acggcaaaaa ttacgtcttc aagaagtcgg acaagcgcaa gctctcctca gacgagatgg
    12901 tcgcctactg gaaatcatgg gccgaacaat acccaatcat ctccatcgag gacggcatgg
    12961 gcgagagcga ctgggacggc tggaaactga tgaccgatga gatgggccgc actgtccaac
    13021 tcgtcggcga cgacctcttc gtcaccaaca cggagttctt acgccgtggg atcgagcgcg
    13081 gcgtcgccaa ttcgatactg attaaggtca accagatcgg cacgctcacc gagactctgg
    13141 acgcgataga gctggcgcgc acgcacggcc tcacggccgt cgtctcgcac cgctcgggcg
    13201 agaccgagga caccttcatc gccgacctcg ccgttgcgac taacgccggc cagatcaaga
    13261 cggggtcgct ttcgcgcacc gaccgcgtcg ccaagtacaa ccaactcctc cgcatcgaag
    13321 aagacctccg cacggccgcc cgctaccccg gcctcgacgc cttctaccag atcgacctca
    13381 ccgcgcggcg agaggataag cccgccgcct cacgtccttc atcgaagaag gcgcgaacgg
    13441 caaagaagtg agcgacgcag tttgagactc agatcaggag atgacggaca ttgactgaca
    13501 gacgcggccc gctcgcgctc atcatcctcg acggcttcgg ccacagcgac gtgcgcgagg
    13561 gcaacgccgt cgcgctggcg cggatgccct tctacgacga gcttctggag aagtacccgc
    13621 acacgctcat cgaggcgtcg ggcgagtgcg tcggcctgcc cgcgggtgtg atgggcaact
    13681 cgaacgtcgg ccacctctgc atgggggcgg gccgcgtcgt ccgcacggac atcgagcgca
    13741 tcaaccacga ggtcagttcg ggcgcgttcg actcgaacct cgcactcgag tcggccgtcg
    13801 agagcgcgct caaacacgac cgcgcgctgc acctcatcgg cctcacctcc gacggcctcg
    13861 tccactcctc gcaggaacac gcctacgccc tgctgcgtct ggccgagcgg cgcggcctgc
    13921 ggcgcgtcta tgtccactgc ttccttgacg gccgcgacac gccgccctct tcggccgacc
    13981 gatatgtccg ccagatgcag gagaagtgcg ccgagatcgg ctgcggcgag gtggcctcgg
    14041 tcgtcgggcg ctactacgcg atggatcgcg accggcgctg ggagcgcacc gagcgcgcct
    14101 accgcctgct cgtccacggc gagggcgcgc gcgcgtccga ccccgtcgcg gcgattaaaa
    14161 gctcctacga gcgcgccgtc accgacgagt tcgtcgagcc ggtcatcgtc acacgcggcg
    14221 agggcggcga acccgtcgcg accattgccg ccggcgactc cgtcatcttc ttcaacttcc
    14281 gcgccgaccg cgcacgccag ataaccagcg ccctcgccgt ccccggcttc gacgacttcg
    14341 cagtccccaa ccggccgcac acgcacttcg tctgcttcgc cgtctacgac aagacctacc
    14401 cgttgcccgt ggccttcccc ccgacgcagc ccacgaacat cctcgccgaa gtcttcgccg
    14461 ccgccggcgt ccgcaactac cgcatgtccg agaccgagaa gtatgcccac gtcacatact
    14521 tcttcaacgg cggcaacgag cgcgagtacc cgcacgagcg acgcctcctc gtcccctctc
    14581 ccaaggttgc cacctacgac ctcgcgcccg agatgtcggc cttcaaggtc acggacaagg
    14641 ttctgcgcgc catcgaggag ggcgacaccg acgtcttcat cctcaacttc gccaaccccg
    14701 acatggtcgg ccacaccggc aacctctcga aaaccatcga ggctgtccag cacgtagaca
    14761 cctgcctcgg ctggatcacg aaggcgctca agaccgcgcg cggtcgcgct atcatcaccg
    14821 ccgaccacgg caactgtgag cagatgatcg accacacaac gggccagccg cacaccgcgc
    14881 acacctccaa ccccgtcccc ttcgtcctcg tagaccacga ctcccgcggc gcacgcctcc
    14941 gcgatgatgg cggcctcgaa gacgtggccc ccaccatgct cggcctgctc ggccttgaga
    15001 agcccgccga gatgaccggg cgcgacctgc gcttgactga ttgaggtcgc gcgcaagcgc
    15061 gcccggcatg cgcggcgcgt aaatgaaacc aacgcaacgc gccgacgtat gttgagggtg
    15121 atgaattccg acccgcgcaa aattctcgtc atcgacttcg gccagttggg cgacgtggtc
    15181 ttgagcctgc cggcgctcgc ggccgtgcgc gggcggtttc cgcgcgcgcg cgtcaccgtg
    15241 gccgtgggta agccgggtgt cccggtggtc gagatgtcgg gatacgcgga cgaggtgatg
    15301 ggcgttgacc gcgtcgcgct gcgcgatggg ccgaagccga tgtcgatttt gcgtatcgtg
    15361 cagttcgtca aagacgtgcg gcgcgagcgt tacgatctcg tcatcgacct gcacagcctc
    15421 tcggagacaa acctgctcgg ctttttttcc ggcgcgagtc agcgtctttt cgcacgccgt
    15481 ccgggtcgct cgctcgactt cctttcaaac tttcacacgc gcccgcccgt ggaggacgcg
    15541 aagaagcacg ccatcgaccg ctacctcgac gtgctgcggc ccctcgacgt gggcgaggtg
    15601 tcgcgcgtgc cgcgtctgcg aacgcgtgcg gaagaccacg cggcggttga tgagtttttg
    15661 aagcaggagc tgccgaggaa ggagcgcgcg cgcagtagta atagtagcga cgacgcgccg
    15721 ctcgtcggcc tctttcccgg cgcgggccac ccttcgcgcc gctggccgct ggagcgtttc
    15781 gcggagctgg cgcgccggct cgaaggcaac gactcggtgc gcgcgttgct cttcgccggt
    15841 cccgaagagc gccgtgtggt gcgcgaggcc cgcgcgcagt ttccgcgctc gaccatcctg
    15901 ctcgaccgcc tcagcgtccc gcagctcgcc gccgcgcagg agcgcctcgc cgtcttcgtc
    15961 tcgaacgaca cggggccgat gcacatcgcc gcggccgtgg gcacgcccgt cgtcatcctg
    16021 atgcagcacc acgcggcctt cgagtgttac atcccgccgg gcgagcgcca tcgcgtcgtc
    16081 cacgctccca ccattgagga gatcacgacc gacgcggcct acacagcgac acgcgcggcc
    16141 ttcaccgccg agcgcacggc ctcgcttttc gcgagctaaa ggacaatggg ggcaacacac
    16201 cagagcaggc gcacgcgccg cgcgcggggc gccgccgtcg ccgcactttt tttactgacc
    16261 acttttacgt cggttgcctg ccgcgacagc agcagcagca cgggtgcgaa cgaaggcgcg
    16321 gcgttgccag ccgagtcggc cgcgggcgaa gacgcggcgc gcggcggacg cgcggcgcgc
    16381 accgcggaag agactcgcaa gggcgacgcc gcgctcaccg ccgagcttga ccgccagatt
    16441 gacgagcgcg ggccggccgc ggcgcgctgg ggcatctcgg tcgtctcgct gcgcgacggg
    16501 cgcgtcctct acgagcgcga cgccggacgc ctgttcacgc ccgcctccgt gatgaaggtc
    16561 tacacgacgg ccgtcgcgct cgatacgctc ggcgcggact accgctggcg cacgtccgtc
    16621 tacgccgtct cggcgccgac tgccggcggc acaatcgaag gcgacctcgt gctctacggt
    16681 cgcggcgcgc ccgacctcac ctcgcgcgac acgcgcgccg gcccttcgca actcgacgag
    16741 ctggctgaac aactccgtcg gcgcggcgtg cgccgcgtgc gcggcgacgt ggtcggcgat
    16801 gagagttatt ttcgcggcga gacactgggc gagggctggc tctggcagga cgtgcagtgg
    16861 tactacggcg ctgagccgag cgcgctcacc gtggacggca acgagatcac ggtcagcgtt
    16921 gcgcccggcg cgaacacgga cgaccccgtc gaggtcaaga cggttccgcc caccgactac
    16981 ctgcgcgtca cgaacgacat gcgaacggtc gagcgcggcg gacggccccg catcggcatc
    17041 acgcgcggac tgtcgtcgaa cgacgtgcgc gtgtggggcg agttccctgc gggcggccct
    17101 ccttacggcg tgcgcctctc cgtccaccgc cccgcgcttc tcgccgcgcg ccttctgcgc
    17161 gccgcgcttc gctcacgcgg catcgaggtc gcgggcgagg cgcgctccgt ggacgcgcgc
    17221 gtgcccgaag acgagagact tgactcggac cgggccgttg agctggcgca cgtcttgagc
    17281 cggacgctcg gcgaggtcgt ccgcgagacg aacaaggaga gcgtgaactt ggacgccgaa
    17341 ctcatcctgc gcgcagtcgg gcgcgagcgt ggcgacacgg cccccgccgc ggacgagcga
    17401 cggacgcgcc agcgcgacac ggtcgtcgcg ggcctcgccg tcgtccgccg ctggctcgaa
    17461 ggcgcgggcc tcgacaccga gccgctcgcg ctccacgacg gctcgggtct ctcgcgtttc
    17521 gacctcgtca cccccgaggc gaccgcccgc ctcctcgcgc acatctcgaa gaccccctca
    17581 gccgcagtct tccgcgactc actccccgtc tctggccgcg acggcacact ccagggccgg
    17641 ctgcgacagg ccgacccgcc catcgaggcc aagaccggaa cgctcgtcta cgtcaacggc
    17701 ctcgccggtt acgccaccac cgccgacggc gaacgcctcg ccttcgccat cttctgcaac
    17761 gacaagaccg agtcccaatc ctccttgcgc gccatcgacg ccgtcgccct cacactcacc
    17821 cgctatccac gccccgcaga gctgcgctag cgacattttt ctacattttt ttgttgaatg
    17881 tagacaaacg atatttatta tgtataatgt tgttgccgcc gcgcggctga gttttgtgcg
    17941 gcgaccggct cccgggaggt tagacggggc gccgcgcgtg agagttcaga aggggcgcca
    18001 aggcgctgca actcttaccc ttacagctcg tcacctgccc ttccagcggt cgtccgaacc
    18061 cacgtccgcg gccccgtaag agaaaaccca tttgccgaaa gaactctccc gacggtgtcc
    18121 gacccgcg
//
LOCUS       DASVES010000031        14086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1129558, whole genome shotgun sequence.
ACCESSION   DASVES010000031 DASVES010000000
VERSION     DASVES010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129558"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF240_01295"
     CDS             <1..197
                     /locus_tag="VF240_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173905.1"
                     /translation="ASFCPQCGRNLQQSAQQFSPQQKAPPPFGPPPPLPGPGGRSKND
                     QMMMILLAVIAACIILGFFC"
     gene            207..755
                     /locus_tag="VF240_01300"
     CDS             207..755
                     /locus_tag="VF240_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173906.1"
                     /translation="MFRYLRDPLFLTSVALYALNRWVVKPSLPAGELFFSGHFNDLLV
                     IPCALPPLLLLHRRLGLRYADAPPTASEVAVHVAVWSVFFEILAPIFVRAARSDAWDV
                     VAYCAGGLASWIVWNRAALTAMIFASRNLIEFFTPDTGRPVYPHDEKIESRSLHSPLT
                     PHQHRRVRAEEEVLRGRPPFPV"
     gene            730..1227
                     /locus_tag="VF240_01305"
     CDS             730..1227
                     /locus_tag="VF240_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173907.1"
                     /translation="MAAPPSPFKHSGQIVTSFDSAARGMRTTLQHPRPLGGSHETIYL
                     TASFIHQDPRRGVRPTVELALVSALKTQKYRDSHGVVLVGDGRPVPLSVAARYQSRPG
                     DQGVIFEVFQLSLSADDLAGVTGARKVTARVGGEEFELTQNHLEALREMASLLGGAPK
                     GWRTE"
     gene            complement(1318..2247)
                     /locus_tag="VF240_01310"
     CDS             complement(1318..2247)
                     /locus_tag="VF240_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173908.1"
                     /translation="MSVLFVCLACAAGYGQAGRGTDGERRRGVRSVTIPVTVRLPKER
                     AQPEEFQYIEGIKVFEDGEPQEILSIRGMAQSPLTLAVLIQDDVVPSVGNEIKGLAEF
                     IRRLPPNSRVLVGYMRAGSIQVRQKFTVDLERAAKSLRIPIGSTSVSPYNPFSTLREA
                     IKRFEGQPIGRRAVLLVSDGLDLSHGVSGSTPGQSPDLQRAIEEAQRRSIAVYSIYAP
                     TVGGAASAVGLAGNGQSALNRLSTETGGRAYFQGTGAPVSFDPFLREVSQLLSRQFAI
                     TYLSTHPMKGFHRIRVASEMTEGDIVHPTGYPR"
     gene            complement(2364..3197)
                     /locus_tag="VF240_01315"
     CDS             complement(2364..3197)
                     /locus_tag="VF240_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173909.1"
                     /translation="MDVDLPEEVEAYRDRVWRREPERRVESALEAEKLVEEVGFCASM
                     TDARRPGPSLYIAVCGRRDAHMPRNVQKDPESSQTWVLKDEVMRRGRVYYAKIAKART
                     VFVAPRLIPHFNALWGVPRRKESEVLSAEARAILKVLRREWEMATADLRRASKVEERN
                     RFTRALDELQRTFKVAPQEVLYEPWFTYIWALPEARFHRELSVKVSRAEALREVARAF
                     LDGAGMTIRGELAKVTGLSRPDAGLGNRALVAEGYAVRVGDGVYRLADLEERVRAHVE
                     L"
     gene            3536..6364
                     /locus_tag="VF240_01320"
     CDS             3536..6364
                     /locus_tag="VF240_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX7173910.1"
                     /translation="MLNEVSTGQQLSHPSPRVNAQRRNTSAALLRAAARKLAAVVVVA
                     CLLCSPAAALSSKGQKNFKRGMEHEAAQQWEKAVEEFALAVAADPSNAEYQLHYRRSV
                     FNASQLFMRQGGALAERGDYIGAYNAFRRAHDYDQANALARSMMERMYAVQLNKATAG
                     AGGEVAIANDPSVATVSPAGYEPAAPARRPSRTPAQQRQSELPQQSGRAEQLQVITYS
                     GDLESFIKYLAKQLRLNAVFDKDFPRRAIEVDLSDVTAAQALDYVFLTQGLFFQKLTS
                     RTILVADQTKRPQYQQLVLKTFYLSNIDPNEARTLIQASIPPQAGRQPVITANKTTNS
                     ITVRDTVENVRLVSELIRSVDKDRAEVVMDVQIYEVSRTDLMQIGNQVGGESSLTNFG
                     GIQPGSVLLGGARQLASAASSVLPLSTGIGLLIPQSSLSAFQRKDNTRLIASTQVHAF
                     DGEKSTAHIGQRVPVQTATITPFGTAGGGESKPQEGVAQGLFGANGYPVIQYEKTGLT
                     LEFTPQVAPNLDVQVKMSIKSNDVSSVANATLTPTFTERNIEGTARIQNNRTMMIASV
                     AQNQQSRGRQGLPLLGLVPILGRLFTAPRRNDQQTDIVIAVTPHVLRAPTYTPRDEEM
                     RPSGTMQTPNSDTLEAMVHDVEREEQIAAARSLPTHSKVELPDAVRQPALAVQHNTAV
                     AAHDTAIQHTPVDQPSFVPAPRALMNSGARIDEGNAAREATMSAAVVIKETGSPVDNS
                     PEVPATTRPRVVEAKFDSPGGAQSVEKIPASDNISAGDNARPFSPDASLYSAPVSAPA
                     APPRSAAQLMLMPEPQRLKVGERRQLKLVLKTDAPLGMIASSLRFDPRVITVRAVSHG
                     NIFADPTALPNVTYSVSTEGVLIFSVAPPTGRKAFTGAGVLLNIEIEAVAPGACDISF
                     GAEDVHLMATDGRKILLRVLPGAVAVAQ"
     gene            complement(6505..6882)
                     /locus_tag="VF240_01325"
     CDS             complement(6505..6882)
                     /locus_tag="VF240_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7173911.1"
                     /translation="MSSLLIVDDDEAVRDTLLDLFSDNHLCHGAPTVETALSFLEVET
                     YDVVLTDISMPGLSGVELLGIIRQRQPETPVIIISGISDPAHAEGLIKLGAFDYILKP
                     FRLEQIEASVARAVEHRRLARQG"
     gene            complement(7224..9179)
                     /locus_tag="VF240_01330"
     CDS             complement(7224..9179)
                     /locus_tag="VF240_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX7173912.1"
                     /translation="MSLGMVSTAQAQRRTYRLGDRQVDSLITRIEQNTDRFRASVDAG
                     LDRSRYDGTRAEDNVNNFIRDFEAATDQLRERFNGRRSVAADVENVLAKAAFINDFMA
                     RTRLTTRAQNDWALVRTDLSALASAYNVVWAWDRQQSTYPTPGTYPTTGGDQRAYRLG
                     DREVDTIIRRVEQNTDRFRASIDAALDRSRYDNTRAENNINEFVRNFETSTDRLRSRF
                     NSRQSVDEDVLGVLNAGASIDTFMRNNRLTNRVQTDWSTVRTDLTALADAYAVAWNWN
                     NYPNNNPTYPTNTYPGTGVGVGTNRLTGTYRLDPSRSSNPRDEAERATSSLPAGDRQR
                     VYDRVLARLEAPEQIAIDRRGRTVQIASTRAPQATFEADGTEREEPLPNGRTSRVRAN
                     LVGDRLEVRSTGFRESDFTVMFDPIDRGRGLQVTRSFYSERLTQPVQVVDYYTKVSDV
                     AQFDIYNGSGGNYPSGTSSTLPTGEFLFRDGETVVATLESELSTRTVQPGQRITLVVR
                     QPSQYEGAVIEGAVASSGRGGRVTGRSEMSFDFDTIRLRDGRSYRFAGFVESVRTANG
                     EVVKVDNEGTVRDNDQTNKTVQRTAIGTAVGAIIGAIAGGGKGAAIGAIVGAAGGAGS
                     VYVQGRDELDLPLGTEFTLRSSAPRNR"
     gene            9685..10119
                     /locus_tag="VF240_01335"
     CDS             9685..10119
                     /locus_tag="VF240_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7173913.1"
                     /translation="MAGEDRKAVVHFAGDGLFIGTSPGGHAQVIETDSARASAATPME
                     MLLLALGGCTAVDVISILHKKRERVTGYHVEVSGDRRDEHPRKYTRLVVRHVVRGHNV
                     SEKAVRQAVELSEEKYCSVAATLRPAAEIVSSYEIVEEDGGA"
     gene            10240..10398
                     /locus_tag="VF240_01340"
     CDS             10240..10398
                     /locus_tag="VF240_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173914.1"
                     /translation="MSDKEQKTCANAVCTCPADEGSKYCSAYCEGIGRNATTIDCNCG
                     CESCGSNI"
     gene            10589..12094
                     /locus_tag="VF240_01345"
     CDS             10589..12094
                     /locus_tag="VF240_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7173915.1"
                     /translation="MMAVTAQLLLVCASALSFTPACARMETASTTNAAAPPVAAIVYT
                     ESADPIVLTPEEQTEAALDSVRRADRDALGANGQLPALTPQEHMRRGAIYHANRAFEE
                     ARAHWRIVLARYASDPNAPMALFLIGRSLFQERRYEEALPHFERLGTESPGTPAGRDG
                     FYYVAATKLRLGRAAEAAQRYIEYVEKFPNGERAEQSYLNIIDSLREAGRHDEAIPWV
                     ARTRQRYPGTVSDTNALFARLRLDVSRGDWQSALQTGDELSRVGFQRGVATSKNEVTY
                     LRAYSLERLGRREEAAAAYRSIPDSLSSYFGGLATARLREFGGDARNAAAEREARVRS
                     EAQRAAADYPAPFREIILRAVRGRAVDPRLMLAIMRQESGFKPTAKSGAGARGLMQLT
                     MDAANRFGPGVQMNNLNEDALYRPEVSITVAAAYLDELAKMFPGLPEAVAASYNGGED
                     NVARWVKRVGQKDNGVFTADVGFTESKDYAQKVLANYRAYTLLYSDKLARK"
     gene            complement(12231..13115)
                     /locus_tag="VF240_01350"
     CDS             complement(12231..13115)
                     /locus_tag="VF240_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173916.1"
                     /translation="MKSVFSLVSALSLFFSFTSAPAAGSGPQERRIYYPPAYSNFTVD
                     VLINGSPAEKLYGRGRSYVEAAEGAEYELRVRNPLPVRVAVALSVDGLNTIDARRTSS
                     WDAKKWVIEPYGTIRIGGWQMSTERARKFYFTTERDSYAARLGRASDLGVITAVFYRE
                     TAPAVTYGRGRTPYPADEPMRRGEDERDERATNAPAPQSAGKGKTNRNTAPSEGLRTD
                     DDYAATGIGRSVNNHVYRVDMSLERRAAAEITIRYEYRPALVRLGLLPRPRPYNDDPL
                     RRRERARGFNDTDFSPEP"
     gene            complement(13353..13859)
                     /locus_tag="VF240_01355"
     CDS             complement(13353..13859)
                     /locus_tag="VF240_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX7173917.1"
                     /translation="MSTRRADAGEVGAHAEADGGDDDHAARRSQRLDALTAVLALVGL
                     ADAVYLTAEHLSGRSVVCMVTSGCDAVLQSQYASLPGGWPLASLGALAYFAVFSLATL
                     SAFGYAHTSTLLRVVVAAMFAFTLWLLYVQAFVLEAFCSYCLLSAAVTTTLAALQVAR
                     LLLRPKNA"
     gene            complement(13856..>14086)
                     /locus_tag="VF240_01360"
     CDS             complement(13856..>14086)
                     /locus_tag="VF240_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7173918.1"
                     /translation="KFAEYAQRAGVSDVERFKTDLESQSVSARIVADVRRASSLGITG
                     TPSVLINGVQVRGVTESELRREIETALAGAGK"
BASE COUNT         2448 a         4760 c         4444 g         2434 t
ORIGIN      
        1 ccgcttcatt ctgcccgcag tgtgggcgca accttcagca gtcggcgcag cagttttctc
       61 ctcaacagaa agcaccgccg ccgttcggcc cgccgccccc tctgcccggc cccggagggc
      121 ggagcaagaa cgatcaaatg atgatgatcc tgctggcggt catcgccgcc tgcatcattc
      181 tcggattctt ttgctgagcg agtaggatgt ttagatatct ccgagaccca ctattcctga
      241 cgagcgtcgc gctctacgcc ctcaaccgct gggtcgtgaa gccttccctt cccgcgggcg
      301 aactcttctt cagcggccac ttcaacgatc tgctcgtcat cccctgcgcc ctgccgccgc
      361 tgctccttct ccaccgccgg ctcggcctgc gctacgccga cgccccgccg acggcctctg
      421 aggtcgcggt acacgtggcg gtctggtcgg tcttttttga aatcctcgcg cccatcttcg
      481 tccgcgcggc gcgctccgac gcgtgggacg ttgtggctta ctgcgccggt gggctggcca
      541 gttggatcgt ctggaacagg gccgccctga ccgcaatgat ttttgcctcg cgtaacctca
      601 tcgagttttt tacccctgac accgggagac ctgtttaccc ccatgatgag aaaatcgagt
      661 ctcgttcttt gcacagccct cttactcctc atcaacaccg gcgcgttcgc gcagaagaag
      721 aagtattgcg tggccgcccc cccttccccg tttaagcaca gcgggcagat agtcacgagc
      781 ttcgactccg cggcccgagg gatgcggacg acgctccaac acccgcgccc actcggcggc
      841 tcgcatgaaa ccatctacct gacggcctcc ttcattcatc aggacccgag gcgcggcgtg
      901 cggcccacgg tcgagttggc cctcgtctcc gccctcaaga cgcagaagta ccgcgactcg
      961 cacggcgtgg tgctcgtcgg cgacggcagg cccgtcccgc tctcggtggc ggcgcgctac
     1021 cagtcgcggc ccggcgatca gggggtgatt tttgaagtgt tccagctctc gctctcggcc
     1081 gacgatctgg ccggcgtcac gggcgcgcgc aaggtgacgg cgcgcgtggg cggcgaggag
     1141 ttcgagctga ctcagaacca cctcgaagca ctgcgcgaga tggcgagcct gttgggcggg
     1201 gcgccgaagg gctggcgcac ggagtagaga agaaggcaaa agtaaaaagg caaaaggcaa
     1261 aagtgaagag gcgagacgct cctcactttt gccttttcct ttttactttt gccttcttta
     1321 gcgcggatag ccggtcgggt ggacgatgtc gccctcggtc atctccgacg ccacgcggat
     1381 gcggtggaag cccttcatgg ggtgcgtcga gaggtacgtg atggcgaact ggcgcgagag
     1441 cagttggctg acttcgcgca ggaacgggtc gaagctgacg ggcgcgcccg tgccctggaa
     1501 gtaggcgcgg ccccccgtct cggtcgagag gcggttgagc gcgctctggc cgttgcccgc
     1561 aagccccacg gcgctcgcgg cgccgccgac ggtcggcgca tagatcgagt agacggcgat
     1621 actccgccgc tgcgcctcct cgatggcgcg ttgcaggtcg gggctttgcc cgggggtgga
     1681 gccgctaacg ccgtgcgaga ggtcgagacc gtcgctgacg agcagcaccg cgcgtcgccc
     1741 aatcggctgc ccctcgaaac gcttgatggc ctcgcgcagc gtcgagaacg ggttatacgg
     1801 cgacacgctc gtcgagccga tggggatacg caacgacttg gcggcgcgct ccagatcgac
     1861 cgtgaacttc tgccgcacct gaatcgatcc cgccctcatg tatcccacta acacgcgcga
     1921 gttcggaggc agccggcgga tgaactcggc gagaccctta atttcgttgc cgaccgaggg
     1981 aacgacatcg tcctggatca gcacggcgag cgtgagcggc gactgcgcca tcccgcggat
     2041 ggacagaatc tcctgcggct ctccgtcctc gaagaccttg atgccttcga tgtattgaaa
     2101 ctcctcgggc tgcgcgcgct ccttcgggag ccgtaccgtc acgggtatcg tgacgctcct
     2161 cacgccccgc cgtctctcgc catccgtgcc cctgccggcc tgaccgtagc cagccgcgca
     2221 cgccaggcac acgaacaaca cactcaccaa ggccctcatc acactgcgtt cggctctcat
     2281 tgcgtctccc gaattccttt ggttcgccgg actgcgaaac attatagctt gccgtcaggc
     2341 cgaatgctca ctcccgcgag tttttacaat tcgacgtgcg cccgaacgcg ctcctcaagg
     2401 tcggccagcc ggtagactcc atccccgacg cgcaccgcgt atccttctgc gacgagcgcg
     2461 cggttgccca gtccggcgtc gggacgcgac agccccgtca ccttcgccaa ctccccacgg
     2521 atggtcatcc cggcgccgtc gagaaaagcg cgggctactt cgcgcaacgc ttcggcccgg
     2581 ctcaccttca cggaaagctc ccggtgaaaa cgcgcctcgg gcaacgccca gatgtacgtg
     2641 aaccacggct cgtagagaac ctcctgcggg gccactttga atgtccgctg tagctcgtcg
     2701 agcgcgcgcg tgaacctgtt gcgctcctcc accttcgacg cgcgccgcag gtcggccgtc
     2761 gccatctccc actcgcgccg caagaccttc aaaatcgcgc gcgcttccgc cgacaacacc
     2821 tccgactctt tcctgcgcgg cacgccccac agcgcgttga agtggggtat caatcgcggc
     2881 gcgacgaaga ccgtccgcgc cttcgcgatc ttcgcgtagt agacgcgccc acgccgcatc
     2941 acctcatcct tcaagaccca cgtctgactg ctctccgggt ctttctgcac gttgcgcggc
     3001 atgtgcgcgt cgcgccgccc gcagacggcg atatagagcg aagggccggg ccgccgcgcg
     3061 tccgtcatcg acgcgcagaa gcccacctcc tccaccaact tttccgcctc caacgcgctc
     3121 tccacccgac gctcaggctc tcgcctccac acccggtcgc gatacgcctc gacctcctcc
     3181 ggcaaatcta catccatcag cctcacccga caccccggca aattaagccc atcaatcctt
     3241 gacaaacttc ggccaactct gtttaatcaa tgttgaccgc aacctgcaag ggttttaagc
     3301 ggcaacagtt agccttgcaa atggtctttt ttttggaaga cttcggacct gttccagact
     3361 ataactttcg agagccagcc gcccgccgcc ccttcactca cacgaaagcc ggggcgtctc
     3421 cccgtctaag taaaacacga gcacgaacgt ctgaccacga cccgccccgg attgatcgac
     3481 cccgcccatg ctctctgccg aacgcgattg tcgcctgaac cgttggaggt gaaaagtgct
     3541 gaacgaagtt tccacagggc agcaactctc ccacccttct cccagagtta acgcacagag
     3601 acgcaacacc tccgccgcac tcctccgcgc ggccgcacgg aagttagcgg ctgttgtcgt
     3661 cgtcgcgtgt ctgctctgtt caccggcggc ggcgctttca tcgaagggcc agaagaattt
     3721 caagcgcggc atggagcacg aggcggcgca gcaatgggag aaggcggtcg aagagttcgc
     3781 cctcgccgtg gcggccgacc cctcaaacgc cgagtaccag ctccactacc gacgctccgt
     3841 cttcaacgcc tcgcagctct tcatgcggca gggcggcgcg ctcgccgaac gaggcgacta
     3901 tatcggagcc tacaacgcct tccgccgcgc gcacgactac gatcaggcca acgccctcgc
     3961 ccgctccatg atggagcgca tgtacgccgt gcagctcaac aaggcgacag ccggcgcggg
     4021 cggtgaagta gccatcgcga acgacccttc cgtcgccacc gtctcgccgg ccggctacga
     4081 acccgccgcc cccgcacgcc ggccctcgcg cacgcccgcg cagcagcggc agtccgagct
     4141 gccccaacag agcggccgcg ccgaacagct tcaggtcatc acctacagcg gcgacctcga
     4201 aagcttcatc aagtatctcg ccaagcagct ccgcctgaac gccgtcttcg acaaagactt
     4261 tccgcggcgg gcaatcgagg ttgatctctc ggacgtgacg gccgcgcagg cgctcgacta
     4321 cgtctttctc acgcagggcc tcttcttcca gaagctcacc agccgcacca tcctcgtcgc
     4381 cgaccagacg aagcgcccgc agtatcaaca gcttgtgctg aagaccttct atctctcgaa
     4441 catcgacccg aacgaagcgc gcacgctcat tcaggcgtcg atcccgccgc aggccggacg
     4501 ccagcccgtc atcaccgcca acaagacaac gaacagcatc accgtgcgcg acacggtcga
     4561 gaacgtccgt ctcgtctccg agttgattag gagtgtggac aaggatcgcg ccgaggtcgt
     4621 gatggacgtg cagatttatg aagtctcacg caccgacctg atgcagatcg gcaaccaggt
     4681 cggcggcgag tcgtcgctga cgaacttcgg cggcatccag cccggctcgg ttctcctcgg
     4741 cggcgcacgc cagctcgcca gcgcggccag ctccgtcctg ccgctctcga ccggcatcgg
     4801 ccttctcatc ccgcagtcgt ccctgagcgc cttccagcgc aaggacaaca cgcgcctcat
     4861 tgcctccacg caggttcacg ccttcgacgg tgagaagtca acggcgcaca tcggccagcg
     4921 cgtgcccgtg cagacggcca ccatcacgcc gttcggcacg gccggcggcg gcgagtccaa
     4981 gccgcaggag ggcgtcgcgc aagggttgtt cggcgctaac ggctaccccg tcatccagta
     5041 tgagaagacc gggctgacgc ttgagttcac gccgcaggtc gccccgaacc tcgacgtgca
     5101 ggtgaagatg agcatcaagt cgaacgacgt gtcgagcgtc gccaacgcga cgctcacgcc
     5161 gaccttcacg gagcgcaaca tcgaaggcac ggcgcgcatc cagaacaacc gcacgatgat
     5221 gattgcgagc gtggcgcaga accagcagtc gcgcggccgg cagggactcc cgctgctcgg
     5281 actcgttcct atcctcggcc gcctcttcac cgcgccgcgc cgcaacgacc agcagaccga
     5341 catcgtcatc gccgtcaccc cgcacgtcct gcgcgcgccc acttacacgc cgcgcgacga
     5401 ggagatgcgg ccgagcggga cgatgcagac gccgaactcc gacacgctcg aagcgatggt
     5461 tcacgacgtg gagcgcgagg agcagattgc cgccgcgcgc agtctcccga cccactcgaa
     5521 ggtcgaactt cccgacgccg tgcgacagcc ggccctcgcc gtccaacaca acaccgccgt
     5581 cgccgcacat gacaccgcca tccagcacac acccgttgac caaccctctt tcgtccccgc
     5641 gccgagggca ctgatgaaca gcggcgctcg catcgacgaa ggcaacgcgg cgcgagaagc
     5701 gacgatgtct gccgccgttg tcataaagga aaccggctcg cccgtggaca actcgccgga
     5761 agtcccggcc actaccagac cccgagtcgt cgaggctaag ttcgactcgc ccggcggcgc
     5821 tcagtcggtc gaaaaaatcc cggccagtga caatatttcg gccggcgaca acgcgcgccc
     5881 cttctcgccc gacgcctcac tttactccgc acctgtcagc gcgcccgccg ccccgccgcg
     5941 tagcgccgcg cagttgatgc tcatgccgga accccagcgg ctcaaggtgg gtgagaggcg
     6001 acagctcaag cttgtcctga agaccgatgc gccactcggg atgatcgcct cgtcgctgcg
     6061 cttcgacccg cgcgtcatca ccgtccgcgc cgtctcgcac gggaacatct tcgccgatcc
     6121 cacggcgctc cccaacgtca cttactcggt ctcgacggag ggcgtactga tcttctccgt
     6181 cgcgccgccc acgggccgga aggctttcac gggcgccggc gtgctcctca acatcgagat
     6241 cgaggccgtc gcccccggcg cgtgcgacat cagcttcggg gcggaagacg ttcacctgat
     6301 ggcgaccgac gggcgcaaga ttcttctgcg cgtattgccc ggcgcggtcg ctgtcgcaca
     6361 gtaagcctct ctgctcccgc ccgtcgtctg agcgtccggg tcaccttaca cacgaactcc
     6421 catcccttcg cgcggtggcg gctgagggcg atttgcgaat caccttgtct gtcgaaaggt
     6481 gtgcgggcgg tggccgtgtg tcgttcagcc ctgccgggcc agccggcggt gctcgacggc
     6541 gcgcgcgacg ctggcctcga tctgctccag ccggaagggc ttcaggatgt agtcgaaggc
     6601 tccgagcttg ataagcccct cggcgtgagc ggggtcgctg atcccggaga tgatgatgac
     6661 gggcgtctcc ggctgccttt gccgaatgat gccgagaagc tccacgccgc tcaggccggg
     6721 catggagatg tctgtgagca ccacgtcgta ggtctcgact tccagaaaag acagggcggt
     6781 ctcgacggtc ggcgccccgt ggcagaggtg attatcggag aacaagtcta agagcgtgtc
     6841 gcggacggct tcatcgtcat cgacgatcaa gagggaggac atgcaggaat actcctgggg
     6901 tgaggtattt ctttgatcgc ggccgatact tcaacccggc aactccccta ctagacgccg
     6961 gatgagatcg ctaacggaaa tccgtgaggt cggccgtgga cggcggagtt gcgaggtcgc
     7021 cagcaaggtc tgggcgctcc ctgtccggga agagattcta acatcgaatc tttgacggcg
     7081 cagccaaata atctgtcgcg acaatctgtc gcgaatagtg aaaggcgtgg acatccgaag
     7141 gcccgccgcg cgtgtcaagg gacatgcgcg gcgggccttc ggaacttctc ccgcttgtca
     7201 gccggttaac cgggcctcgg cccttacctg ttgcgcgggg cgctggagcg gagggtgaac
     7261 tccgtgccga gcggcagatc aagctcgtcg cgtccctgga cgtagacgga gcccgcgccg
     7321 cccgcggcgc cgacaatcgc gccgatggcc gcgcccttac cgccgccggc aatggcgccg
     7381 atgatcgcgc cgacggccgt gccgatggcg gtgcgctgca cggtcttgtt ggtctggtcg
     7441 ttgtcacgca ccgtgccctc gttgtccacc ttgacgactt cgccgttggc ggtgcggacg
     7501 ctctcgacga agcccgcgaa gcgatagctg cgcccgtcgc gcaggcggat ggtgtcgaag
     7561 tcgaacgaca tctcggagcg gcccgtgacg cgcccgccgc ggccgctgct ggcgaccgcg
     7621 ccctcgatca cggccccctc atactgcgag ggctgccgca cgaccagcgt gatgcgctgg
     7681 cccggctgga cggttctggt cgagagttcg ctctcaagcg tggcgacgac cgtctcgccg
     7741 tcgcggaaga ggaactcgcc cgtaggcagg gtcgatgatg tgcccgacgg gtagttaccg
     7801 cccgagccgt tgtagatgtc gaactgcgcc acgtccgaga ccttagtgta gtaatcaacc
     7861 acctgtacgg gctgcgtcag gcgctccgag taaaagctac gcgtgacttg cagaccgcgg
     7921 ccccggtcga tggggtcgaa catcaccgtg aagtcgctct cgcggaagcc ggtcgagcgc
     7981 acttccagac ggtcgccgac aaggttggcc cgcacgcgcg aagtccggcc gttgggcaaa
     8041 ggctcctcgc gctccgtgcc gtcggcctcg aaggtcgcct gcggggcgcg cgtcgaggcg
     8101 atctggacgg tgcggccgcg gcggtcgatg gcgatctgct cgggcgcttc gagacgcgcg
     8161 agcacgcggt cgtagacgcg ctggcggtcg ccggcgggca ggctgctggt ggcgcgctcg
     8221 gcttcatcgc gcgggttgct gctgcgcgaa gggtcgagac ggtaggtgcc cgtcaggcgg
     8281 ttcgtgccga cgccgacgcc cgtgccgggg taagtgttcg tcgggtacgt cggattgttg
     8341 ttcgggtagt tgttccagtt ccaggcgacg gcgtaggcgt cggccagtgc cgtcaggtct
     8401 gtacggacgg ttgaccagtc ggtctgcacg cggttcgtca gccggttgtt acgcatgaag
     8461 gtgtcgatgc tggcgccggc gttgagcacg cccagcacat cttcgtccac cgactgccgg
     8521 ctgttgaagc gcgagcggag gcggtcggtc gaggtctcga agttgcggac gaactcgttg
     8581 atgttgttct cggcccgcgt gttgtcgtag cggctgcggt cgagcgccgc gtcgatgctc
     8641 gcgcggaagc ggtcggtgtt ctgctcgacg cggcggatga tcgtatcaac ctcacggtcg
     8701 ccgagacgat aggcgcgctg gtcccctccg gtcgtcgggt aagttcccgg cgtcgggtag
     8761 gtggactgct ggcgatccca ggcccagacg acgttgtagg cgctggcgag cgcgctcaga
     8821 tcggtgcgga cgagcgccca atcgttttgc gcgcgcgtcg tcaaacgcgt gcgcgccatg
     8881 aagtcgttga tgaaggccgc cttcgcgagg acgttctcca catcggccgc gacggagcgg
     8941 cggccgttga agcgctcacg cagttggtcg gtggcggcct cgaagtcgcg gatgaagttg
     9001 ttgacgttgt cctcggcgcg cgtgccgtcg tagcggctgc ggtcgaggcc cgcgtccacg
     9061 ctggcgcgga agcggtcggt gttctgttcg atgcgggtga tgagactgtc tacctggcgg
     9121 tcgcccaggc ggtacgtgcg gcgttgtgcc tgcgccgtgc tgaccatgcc aagtgacact
     9181 aaggctacca ccagtgcggc agaaattgtt tgtcttactc tgttcattta attcgtccct
     9241 ccagctttta gaagtccacc tcgaagtaag gatcgcgtcg ggcggtttga aagtcttcgg
     9301 cccacctcac cccgttcttg atagggagtt acaaacaccg ggagagtagt ttggtcgggg
     9361 caaggcgtgc tttatcaaac ccgttacgag gcaagccctg tgccgacaag acgtttaaaa
     9421 tgaccgggga gggtgcgcgc cgcccacaag cgcgccgggg agtgcattcc gtgtctggga
     9481 ttcggcagac caagcgtggg gttgccgcta attgttgaag gagagctttt tgttgtcgcg
     9541 ctctattcag gcggaaatgt acgaatagtg acagccggcg tccgagtagc atacaaggcg
     9601 aaagcccgca ttttcgggcc tgtgtgacgc gcggcgcgcg cgtgatatgc ttgggagcat
     9661 cccacttcgg aggtgcgtca gagcatggca ggagaagaca ggaaggcggt cgtccacttc
     9721 gcgggcgatg gactcttcat cggcacgagt cccggcggcc acgcgcaagt tatcgagacc
     9781 gactcggctc gcgcgagcgc ggccacgccg atggagatgc tgctgctcgc gctcggcggt
     9841 tgtacggccg tggacgtgat ttcaattctg cacaagaagc gcgagcgcgt gacgggctac
     9901 cacgtcgaag tcagtggcga ccggcgcgac gagcacccgc ggaagtacac gcgcctcgtc
     9961 gtgcgacacg tcgtgcgcgg gcacaatgtt tcggagaagg cggtgcgtca ggccgtcgag
    10021 ctatccgagg aaaagtactg tagcgtcgcc gccacgctgc gcccggccgc cgagatcgtt
    10081 tcgagttacg agatcgtcga ggaggatggc ggcgcgtgac gaagcggcgt gcttgtccgg
    10141 cgccggcgcg catcaaagcc gagacggcgt gcggacgcgc ccgccctgct gctagaatga
    10201 gtggcacaag ctcttccagc tcccaggaga atttataaaa tgtctgacaa agaacaaaag
    10261 acctgcgcca acgccgtctg cacctgcccg gcggacgaag gcagcaagta ctgctcggcc
    10321 tattgcgagg gaatcggccg caacgccact accatcgatt gcaactgcgg ctgcgaaagc
    10381 tgcggctcga acatctaagt cgtccgcgct cgtccccgcg ccgggacttc aataagcccc
    10441 gcgcgtgcgc gcacgaacga ctcacctctc ccgcaaggcg cggacgcgcc cgcccacaca
    10501 atgcgcgcgg cctcgaattg cccgcgcacg gttccctttg tgggcgcgtc cgcgaatctc
    10561 ccccacagac gttttcacgg actacggaat gatggctgtg accgcccaac ttctactcgt
    10621 ctgcgcctcg gcgctctcct tcacgcccgc ctgcgcgcgc atggagacgg cctccacgac
    10681 caacgccgcc gcgccgcccg tcgccgcgat tgtctacacc gagtcggccg accccatcgt
    10741 gctcacgccc gaggagcaga cggaggccgc gctcgactcc gtgcgccgcg ccgaccgcga
    10801 cgcgctcggg gccaacgggc aactacccgc gctgacgccg caggagcaca tgcgccgcgg
    10861 cgccatctac cacgccaacc gcgccttcga ggaggcccgc gcccactggc gcatcgtgct
    10921 tgcgcgctac gcctccgatc cgaacgctcc gatggccctc ttcctcatcg gccgctcgct
    10981 ctttcaggag cgccgctacg aggaggcgct cccgcacttc gagcgtctcg gcacggagtc
    11041 gccgggcact ccggccgggc gcgacggctt ttactacgtc gccgccacga agctgcgtct
    11101 cggacgcgcc gccgaggccg cgcaacgtta catcgagtac gtcgagaaat tcccgaacgg
    11161 cgagcgggcc gaacagtcgt atctgaatat catcgactct ctgcgcgagg ccggtcgcca
    11221 cgacgaggcg attccctggg tcgcgcgcac gcgacagcgt taccccggaa ccgtgtcgga
    11281 cacgaacgcg ctgtttgcgc gcctgcgcct ggacgtttcg cgcggcgact ggcagtcggc
    11341 gctccagacg ggtgacgagt tgagccgcgt aggctttcag cgcggcgtcg cgacgagcaa
    11401 gaacgaggtc acttacctgc gcgcctacag cctcgaacgg ctgggccgga gggaggaggc
    11461 ggcggccgcc taccgttcga ttcccgattc gctcagttcc tatttcggcg ggctggccac
    11521 ggcgcgcctg cgcgagttcg gcggtgacgc gcgaaacgcc gccgccgagc gcgaggcgcg
    11581 cgtgcgctcc gaggcgcaga gggccgcggc cgactacccc gctccctttc gcgagatcat
    11641 cctgcgcgcg gtgcgcgggc gcgcggtcga tccgcgtttg atgctcgcga tcatgcggca
    11701 ggagagcggc tttaagccga cggccaagtc gggcgcgggc gcacgcggcc tgatgcagtt
    11761 gacgatggac gccgccaaca gattcgggcc gggcgtccag atgaacaatc tcaacgagga
    11821 cgcactctat cgcccagagg tctccatcac ggtggccgcc gcttacctcg acgagctggc
    11881 gaagatgttt ccggggttgc ccgaggccgt cgccgcctcc tacaacggcg gcgaagacaa
    11941 cgtggcgcgc tgggtcaagc gcgtcggcca gaaggacaac ggcgtcttca ccgccgacgt
    12001 gggcttcacc gaatccaagg actacgccca aaaggtgctc gccaactacc gcgcctacac
    12061 actgctctac tcggataagc tcgcgcgcaa gtaagacgaa agctcgtcaa tcgtcaatcg
    12121 tgaatagtga atcgcgattg agtagccgcc cggcttgagt tgtggaactc aagccgggcg
    12181 gctactcaac taaatggatt gacgattgac gattgacgat tgacgagctt ttacggctcg
    12241 ggactgaagt ccgtgtcgtt gaagccgcgt gctcgctcgc ggcggcgcag cgggtcgtcg
    12301 ttgtaggggc gggggcgcgg gaggagtccg agccggacga gcgccgggcg atactcgtag
    12361 cgaatcgtga tctcggcggc ggcgcggcgc tccaggctca tgtccacgcg atagacgtgg
    12421 ttgttgaccg agcggccgat gccggtcgcc gcgtaatcgt catccgtccg aagcccctcg
    12481 gacggggcgg tgtttctgtt cgtcttaccc ttgcccgcgc tttgcggggc gggcgcattc
    12541 gtcgcccttt catctctctc gtcttcgcct ctacgcatcg gttcatcggc ggggtaaggc
    12601 gtccgccccc tcccgtaggt gacggccggc gcggtctcgc ggtagaagac ggccgtgatg
    12661 acgccgaggt ctgacgcgcg tccgagacgc gccgcgtaag agtcgcgctc ggtcgtgaag
    12721 taaaacttgc gggcgcgctc cgtgctcatc tgccagccgc cgatgcgaat cgtcccgtaa
    12781 ggctcgatga cccacttctt ggcgtcccag ctcgacgtgc ggcgcgcgtc gatggtgttg
    12841 aggccatcga cggagagggc gacggccacg cgcaccggca gcggattcct gacgcgcagc
    12901 tcgtactcgg ccccctcggc ggcctcgacg tagcttcgac cacgcccgta aagcttttcg
    12961 gccggcgacc cgttgatgag aacgtcaacc gtgaaattcg agtaggcggg cgggtagtag
    13021 attcgacgct cctgcggccc actgccggct gccggggccg aagtgaagct gaaaaacaga
    13081 cttagcgcgc tgactaaaga aaatacggac ttcatcgctc gaaccctcct caaagataca
    13141 atctccccat tttggagaac gcccccaacg gactttcttg cgccatgcgg cgcgacttca
    13201 gtgtttagtt gagtgtttag tgactcagac tcaccgcaga ggcgcagaga acgcagagtt
    13261 cacgcagagt tgattccagc ctccgcgcct cctctgcgtt ctctgcgcct ccgcggtgaa
    13321 ctaccactcc gtacactcaa cttaacactg cactaggcat ttttcgggcg cagcaacagg
    13381 cgggcgactt gtaacgcggc gagggttgtg gtgacggcgg ccgagagcag gcaataggag
    13441 cagaacgctt cgaggacgaa cgcctgcacg tagagaagcc agagcgtgaa ggcgaacatc
    13501 gcggccacga cgacgcgcag cagcgtcgag gtgtgtgcat agccgaaggc ggagagggtg
    13561 gcgagcgaaa agaccgcgaa gtaggcgagc gcgccgagcg aggcgagagg ccagccgccc
    13621 ggcagagacg cgtactggct ttgcagcacc gcgtcgcagc cagaggtgac catgcagacg
    13681 acgctgcgcc ccgagaggtg ttcggccgtc aggtagacgg cgtcggccag gccgacgagc
    13741 gcgaggacgg cggtcagcgc gtcgagacgc tgcgaccgcc gcgccgcgtg gtcgtcgtcg
    13801 ccgccatccg cctcggcgtg tgcaccgact tcgcccgcgt ccgcacggcg cgtactcact
    13861 tccccgcccc ggcgagcgcc gtctcgatct cccggcgcag ttccgattcg gtcaccccgc
    13921 gaacctgcac gccgttgatg aggacggaag gcgtaccggt gatgccgagc gaagaggcgc
    13981 ggcgcacgtc ggccacgatg cgcgcgctca cgctctggct ttcgaggtcg gtcttgaaac
    14041 gctccacgtc ggagaccccc gcgcgttgcg cgtactcggc gaactt
//
LOCUS       DASVES010000032        32580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1517810, whole genome shotgun sequence.
ACCESSION   DASVES010000032 DASVES010000000
VERSION     DASVES010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 32580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32580
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517810"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2802)
                     /locus_tag="VF240_01365"
     CDS             complement(139..2802)
                     /locus_tag="VF240_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7173919.1"
                     /translation="MSSKKSLSITDTSAPAPRRRMPPPPARRRGILSRFWLPAVILLA
                     ITAGGLTGIVLAYQLNYSKAAEEVKSLATYKPSLITRIYADDGETVIGEFALERRIPL
                     KYEEIPPLIHKAILSVEDVRFYDHVGIDPVRIAGATWKNITTGSREGGSTLTQQLSKN
                     LFLSRKQTYERKITEWVVALQIERYYTKQQIMEMYVNHIFLGANAYGFEAAARTYFNK
                     PAKDLTLEEAALLAGIPRAPSQYSPTVSMETAKERRDLVLDLMARNEFVSASDAQAAK
                     AKPIKLAGTAHGTVQTRSSIFNYPVEEIRQYLEDKYTTRVAQSGLTVYSTINVEAQKL
                     AYDTLRKGLRKYDRGRGWRSEFRNIAGEGTNGNGNGNGNGTAAGPTTNGAAPTPQQLA
                     AYRHPDWYVEKFEVDEYLMGLVVEVNRAKNEAAVRFGPYRAVVTAKDMGWSARQPKDQ
                     FKLGDLAEFKIKEVDENARTLKIELSQVPEVSGAMMTINAKDGEIVSMIGGYDFNLNK
                     FNTATQAYRQTGSCFKPFVYAAAVEWGLTPDSIVSGAPFRDRSSGWQPHNYDGSLSHG
                     NVPMKTALAKSLNLAAVHLLQMVGIQTGAQMVRRFGITSPMAPYLPSALGATEVPLDE
                     MVSAYSAFANKGIRHEKHLIRRVTDRDGTVLEEWEKTTFKVMNEYVAMSMAEMMRGVT
                     SGGGTAAAASSVGHQVAGKTGTVNDHTDVWFIGYTPTYVTGVWMGYPERKQSLGAGFT
                     GGHGALPIWVDYMKEFLKDKPKETFAKAPKMPEDIRELYLQRQREMAEERESQMALRS
                     SYKSGDDDSSLPATVTDPKLEQLTLPPTIGGETKSSAPADATPSKSEPAAPRDDKKDD
                     APPPRPATQPRPRGDTDAPQKKSGKKGSDEP"
     gene            3094..3483
                     /gene="ssrA"
                     /locus_tag="VF240_01370"
     tmRNA           3094..3483
                     /gene="ssrA"
                     /locus_tag="VF240_01370"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3630..4577)
                     /locus_tag="VF240_01375"
     CDS             complement(3630..4577)
                     /locus_tag="VF240_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7173920.1"
                     /translation="MRQFLASLMTAAALALGGGLAPDALGQNAPAGEPVGSTRAASPV
                     KLTLPLKDGSVRFMVVGDTGTGTEQQKELADVMLRYREAFPFEFVLLMGDNMYGGEKP
                     ADFKLKFEDIYKRLLDDKVMFYASLGNHDESNQRFYEHFNMKGEEYYRLQKGNIAFYA
                     LNSNYMDKRQLKWLEEELAKDASEWKIAFFHHPPYSSGGKHGSSTGVREIVEPIFLKY
                     GVNAVFAGHEHFYERLKPQKGIYYFISGAGGKLRPGDVKDNSPLTEKAFDKDMSFMLL
                     EIAGDQMHFQVITRAGETVDSGVLANQRKKATAASAASN"
     gene            complement(4605..5222)
                     /locus_tag="VF240_01380"
     CDS             complement(4605..5222)
                     /locus_tag="VF240_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173921.1"
                     /translation="MPRKLNHRIGFVTGVAVVLALSFSPASSARSAFAQQVHDAPPEI
                     PVKYRKGVAAFEGRVKEYAVMREGMEEKLPKLPKESTPEQIQAHKTAFEEMVRAARIG
                     AKPGDAFKPDVAGYIRATIRKEFQGKERQKLRETVLEADTKGVPLRVNYTYPEAKELV
                     EMPPTLLLKLPQLPKQVRYRFVGRNLLLVDRENGLIVDYMLNAVP"
     gene            complement(5484..6581)
                     /locus_tag="VF240_01385"
     CDS             complement(5484..6581)
                     /locus_tag="VF240_01385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7173922.1"
                     /translation="MKIRITFLLLLVLMLSTAARGGQGDSGAVRVSAHPRRVIVERSA
                     AGQLVNCDLAVENLTDEPLTINEIEVSVFDARGALVLRKFVNDNGGRPSIHVVPVREV
                     EPKQTILVFNPLYFFDHGVDLTRLRFRVSLASKDGKKKQEATTDVSPVAYETKTDLVL
                     PLVGRVLVWDGHDFYSHHRRWDYLHPFLQQLGFRTNAGRFGYDFVHVNERGEMRDGPA
                     ERNESWFAFGRPVLAAGAGRVADAYDRMADDRRFDEAEIMTRELAPAGNYVVIDHGNG
                     EFSIFMHLKRGSVKVKRGDAVTRGQQIAEVGASGSSLFPHLHYELRDGAGYKTVEGLP
                     SYFRGFRRHLGSKSVEVRRGQIDSGDIIEGR"
     gene            complement(6613..7344)
                     /locus_tag="VF240_01390"
     CDS             complement(6613..7344)
                     /locus_tag="VF240_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173923.1"
                     /translation="MEWKPDRLLRLILALTMFTMLTFWLPTVRGLLDGDTYSWGWTLW
                     GINFGGRGLRGDYWVLPLVAAFGVTMLYTGWRGARPPFHWMLLLWNVPAAVEATYNAV
                     RFPDEYRFRGDTMGIDISLAWVGPLIFGSIALASLAWVVRDLRRRERREAPPWTGRNT
                     ALLIATISLLPLQLFLLRSGEPHGTTDKIGVVITMLQWLLLSLSFVPWQRRGGAAQRA
                     DVAKSVKESKAAGSDHNKLSAAAPA"
     gene            7571..8464
                     /gene="murI"
                     /locus_tag="VF240_01395"
     CDS             7571..8464
                     /gene="murI"
                     /locus_tag="VF240_01395"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX7173924.1"
                     /translation="MNDLPIGIFDSGVGGLTVYRALHDRLPEERFVYLGDTARVPYGT
                     KSLATVERYAIENARFLEAHGIKLLVVACNTASALALPAIRESVGVSVVGMVGPGARA
                     VVRAAAGRRIGVIATESTIRSGAYAREIARLAPGAEVVERACPLFVSLAEEGWAETEV
                     ARTVAAEYLQDLRGGSVGALVLGCTHYPILRRVIQETMGEDVRLVDSGEAASEAVAEV
                     LDAEGLRRREYDTHTGRLLKERARVGRLCDDLDHFYVTDAAERFARVAERFLGTAPRV
                     LEAVEVWGTDGLNTAERTVQT"
     gene            8427..9182
                     /gene="rph"
                     /locus_tag="VF240_01400"
     CDS             8427..9182
                     /gene="rph"
                     /locus_tag="VF240_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7173925.1"
                     /translation="MDSTPRSEQFKRSDGRRPEELRSVRITPHFMPYAEGSALIEMGQ
                     TRVICAATLEERVPFFKRNSGQGWLTAEYAMLPRATQQRTQRETGRGGPSGRTHEIQR
                     LIGRSLRAVCDLGALGERTLTVDCDVLQADGGTRTAAITGAYVAFVLACRRLKNAGQI
                     ARLPISGEMAAVSVGIVGGAALLDLKYDEDSRAGVDMNVVGTGDGRFIEIQGTAEGEP
                     FSRDQMDELLALAGAGLTQLFAAQRAALEAPQP"
     gene            9360..9878
                     /locus_tag="VF240_01405"
     CDS             9360..9878
                     /locus_tag="VF240_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173926.1"
                     /translation="MRRNMFLLACVLTLGVCGAQARAQEVFADPNVEYTLELPTPTWK
                     TTSRADGVYQRVEFVNGDRMDGYLRIRKEAINHDETPAAIAARHADLKLRYKSGYVGG
                     GKPESFAGRLKGVTMGYEYTEAGKQMAGRVYYLQADDHTVYALHFTGLREKLQRARNQ
                     TDSIARSFRLKQ"
     gene            10065..10493
                     /locus_tag="VF240_01410"
     CDS             10065..10493
                     /locus_tag="VF240_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX7173927.1"
                     /translation="MKLQLAPGLAVGESKIHGRGCFAAAPFPKGRKIAEYAGELISGR
                     EAERRMRGREVHRVCPLETGRYLDGARGGNGTHYINHSCTPNAYMRLTRGHILFMALR
                     DIRPGEEITIDYFSTHHPDTKRCHCKAPTCRGTINKRRDK"
     gene            complement(10569..12635)
                     /locus_tag="VF240_01415"
     CDS             complement(10569..12635)
                     /locus_tag="VF240_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX7173928.1"
                     /translation="MKHFVVPTLKSRLVSVALSLAVSAFAFALWSSGALVLIGGMFLL
                     SGDAAPAIAPQWWVAALLIGAAGACAGFLVEIVGARRTSLLLACALALMCAASYLASY
                     FLNIDIAFAPAALATLGAFLAVQAWRLWIVDVALSGSVRRVASRSSALEGREARARMQ
                     SGLRLLATVLPLDEAVVCCLDEDGRPTPAAYMRPDAGGAATAASNRNTAWREGIRLCE
                     RAIRSGQMVVQPQTQATGNDSDAHAHRDDAHTTTSVVVPLRHEGRAVGALLIRLRGGF
                     DETDRPLLANVAAQLARDLQRDEARAHSVGAPSETFYSVRAAERRLESFGMLSGLLTE
                     QRFAAHALSESHDGHAVAYLDGTIAYVNAPMREAARMAEGDCDTLDLFGLLDRFRAGV
                     FDEPSIAVRRVLQSGSAYECELPFSERGQTLSLRIALVTDAGEDGSPAARPLCLAVTV
                     RDVTEMKEYEKLKSDMVSLMSHELRTPITSINGFAELLATDDTLPESAREFLGIIHSE
                     SQRLVRMINTFLSVTKLEQKDRQEVLKIPLMLSDVVRETVLCMQPVAKRKRIRLVGSD
                     GSGKLPPVAADRALVTQAVMNIVDNAIRYSPERTTVTLTTALESEAVRVTVEDCGYGI
                     PPDAVDRVWEKFYRVVRDGQDKEEDSAGLGLSFVREVVEQHGGRVGLESEVGRGSKFS
                     FTLPRL"
     gene            complement(12860..14284)
                     /gene="selA"
                     /locus_tag="VF240_01420"
     CDS             complement(12860..14284)
                     /gene="selA"
                     /locus_tag="VF240_01420"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX7173929.1"
                     /translation="MPDETTANVLRALPSVDALLRTPEAQELRERTGAVRLTALARSV
                     TEELRAELSARGADGNGAAARDELLAEAARRLQREAAREGLAGVRRVINATGVVLHTN
                     LGRAPLSEAARAAVAREAASYCTLEYDLAKGERGRRGARAEKLLAELTGAEDALVVNN
                     CAAAALLVLSVLARGGETIVSRGELVEIGGDFRVPDVMAQSATTMIEVGTTNRTRLSD
                     YERAITERTRLILRVHPSNYRIVGFTAAPSLAELAELAHRAGLPLYEDAGSGALIDLT
                     PYGLGDEPVIRDSIEGGADVVTFSGDKLLGAAQAGLIVGRGDVVERLRRHSLYRALRA
                     DKLALAALEATLEAYRRGTATSEVPALRMLSATREEIEVRARAFAEGLRARSEAGALR
                     VEVVEGRSAVGGGSAPTTHPPTALVALSHPVLSADALEEALRHARPPVIARILDGRVA
                     LDLRTVAEDEEAELLDAVACACLS"
     gene            14559..15206
                     /locus_tag="VF240_01425"
     CDS             14559..15206
                     /locus_tag="VF240_01425"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEX7173930.1"
                     /translation="MAKGGNKFTRQAQEREKRSVVARDAQPTVDEQLTRLDDDVRRLK
                     VEFDIFFNGGAKRPPYDTKGRVETLIKRLADDRTLTYAQRYRYNSLVARHTSFLQLWR
                     RTMQGREEGRDPLSAARAATHAKPEPPASVAAPNTFVCADPHTDVPTVKRLFEALVEA
                     KRQCGEGTDDLSFPRFHRMIAAKTDSIKEKLDCERVQFSVSVKDGRVNFKAKAEK"
     gene            complement(15399..15605)
                     /locus_tag="VF240_01430"
     CDS             complement(15399..15605)
                     /locus_tag="VF240_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX7173931.1"
                     /translation="MSRITGKVKWFNNAKGYGFIEQPGSSDIFVHYSAIQGDGFKTLE
                     EGQEVEFEVTQGPKGPQAEKVMKV"
     gene            complement(15966..16823)
                     /locus_tag="VF240_01435"
     CDS             complement(15966..16823)
                     /locus_tag="VF240_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7173932.1"
                     /translation="MMKDERIYAFIVAHTSRSRAGIRRISIHKRWVKVSACAATLVVC
                     AALYGVYGLARQTAHLRIEREYNRLRLENERQRQKLDELKTRIDAIETDARRLSEISG
                     VPAEEGTSPHGAGGPQVLADEATVTAVEVRASQLEGQLRAYEDALRRKLELERIPSIW
                     PVGGEITDDFGVRGNPFGGGSAEFHDGLDIATAWGTPVVATGNGTISFAGVKNGYGLI
                     VIVDHGDGLSSAYGHLSKIEVELGQPIKRGDALGRVGSTGRSTGPHLHYEVRMGESAV
                     SPARYLPMP"
     gene            complement(17219..17440)
                     /locus_tag="VF240_01440"
     CDS             complement(17219..17440)
                     /locus_tag="VF240_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173933.1"
                     /translation="MSAIFSVAARWGGVLTIILLVITLLKQLIALVGFVMVAIKIALI
                     ILFVGLMLLIVLSMLRGRGRRRREEAEEL"
     gene            complement(17591..18385)
                     /locus_tag="VF240_01445"
     CDS             complement(17591..18385)
                     /locus_tag="VF240_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173934.1"
                     /translation="MLTNCSYHLTNRAVVQCSHCARPLCPACDHRIRGFPHCQDCIVA
                     GVELLQQQQARSVASSPQVVRRKTSPFVALLLSLLTPGLGAAYNGQTSKALVHFTIFA
                     SFFQMATVTNGTAFFLLGMVGTWLFAAIDAVRTAQLIRAGLAPDAESDAIARRLYGNP
                     LAWGFVLIALGTVFLLHTLFGIQLPVKQVLPVVLVILGAYMLFDWTRNRQQKREAPYS
                     FEHLTHRPSAIGTGTFGAEATRFGTGEVVTQVSARESNIWPAGPRA"
     gene            complement(18468..19280)
                     /locus_tag="VF240_01450"
     CDS             complement(18468..19280)
                     /locus_tag="VF240_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7173935.1"
                     /translation="MLCTEFEDRLTDYLDGALAVEVQRAFGEHALRCPLCHEILNEVR
                     NTISECRASEAPAPSREFEARILLRTAPETSMTCEDFETHLTDYLDGFLPATLFHRWE
                     RHAALCERCTELPGQVVRSIGACYSYINIEQAVPAGLHERILQATLGTTQAQEVRPPL
                     AARLAERLRGWLDVVVQPQFATVATMVLLAVFVGTNTISEDGSIGGMYRASVRLAAET
                     YSRGASSPDLKRVAEGLETFVGTPARDGAAAVTANDNKSQPAAETPKDEPKK"
     gene            complement(19348..19932)
                     /locus_tag="VF240_01455"
     CDS             complement(19348..19932)
                     /locus_tag="VF240_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7173936.1"
                     /translation="MAVSDGAELVRRCRTGDGAAWEEIVQTFSRRIYNLAYRFTSRAD
                     AAEDLTQEVFVRVYRSLEQYDPRQGDLQNWLMRLARNLIIDDYRRRQRAPQDTHAEDL
                     EGHAYHLRAAGGSPQRELERQELGRQVQAGIDKLPTDLRTCVILRDIEELSYQEIVDL
                     LKIPEGTVKSRINRGRIELAKILRRMRVVAMSDV"
     gene            20349..21476
                     /locus_tag="VF240_01460"
     CDS             20349..21476
                     /locus_tag="VF240_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7173937.1"
                     /translation="MRKYLKFIALSLVAVLLLWWFGHNLDWAEVSASIGRADWRLIAG
                     GVVLIWVTYLIRAYRWRAFLGPLAPGASLREIFAATTVGFSAIFLAGRAGEFVRPAYL
                     PLRDKNVRPGAAFVTIAVERIYDMVAVIVLFAVNLLVFRAPGADAASYERVREAGFVL
                     LLGALAGVAALVYLRRHTAAVTRWIDARLTKAPALLRRAGKILTGLIEQLSHALGVLT
                     DTRELLVTAGWTVALWATIVAANMLVMRAFGLSVGVSGVVFVLGWSLVGSVAPTPGGG
                     AGTFHAATAYGMTAFLNVDETQAKAATIVLHLVVFGSALFFGAYYFFRSHVALARLRE
                     MSDEERAELEGESHPAAPATPGDREAGGGGSAATALRFDRT"
     gene            21593..22069
                     /gene="nrdR"
                     /locus_tag="VF240_01465"
     CDS             21593..22069
                     /gene="nrdR"
                     /locus_tag="VF240_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX7173938.1"
                     /translation="MMCPFCAYLEDKVVDSRESREGDAIRRRRECLRCERRFTTYERI
                     DEIPYMVVKKDGRREPFDRNKVMAGLLRACEKRPVPSAKLDAIVTSIEKYVQESSERE
                     RPTSKVGEMIMRRLKELDKVAYVRFASVYLEFEDVSEFMHELKTLVRARSSAGKKS"
     gene            22179..22652
                     /locus_tag="VF240_01470"
     CDS             22179..22652
                     /locus_tag="VF240_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7173939.1"
                     /translation="MKRIAHTTGLERIELERLRSRVGRLFVMLEEAAEAVSPRVPGAW
                     LPPADVCESEDAVTVRVELPGVRAGQVEVALTNSHLRISGRKRKGAPRAAATHLCSER
                     SYGQFARVIPLRWPVRVTEATAELRDGLLTIRLPKLLERRGGEFKVEVAAGDGDE"
     gene            22709..25189
                     /gene="lon"
                     /locus_tag="VF240_01475"
     CDS             22709..25189
                     /gene="lon"
                     /locus_tag="VF240_01475"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7173940.1"
                     /translation="MADKDFMAAITDTADGVVVEAQDAPGGVEQQLQVPDVLPVLPLR
                     DIVIYPFMIVPLFVSREKSIRAVDEALGENRMILLVSQRDLDKEEPAGEDLYMTGTVA
                     VIMRMLKLPDGRIRILVQGLVRARVESVEDVEYLKARVSVMPEPPAPERSLEVEALMR
                     NVRGSVEKAANLGKNISPEVMAIVANLDEPGRLADLAASNLELKVEDAQSVLDIADTT
                     ARLKRVNELLNKEIEVLTVQQEINTQARADIDRSQREFYLRQQMKAIQSELGEGNELA
                     EEIAQFREKIEASRMPKHAEEEALRQLKKLERMHPDAAETATLRNWMEIMVDLPWSKA
                     STDNLDLIKAQTILDEDHYGLEKVKDRIVEALAVRKLKEKPKGSILCLVGPPGVGKTS
                     LGRSIARALDRKFVRLSLGGLHDEAEIRGHRRTYVGAMPGRIVQALQQAATNNPLIML
                     DEIDKVGADFRGDPSSALLEVLDPEQNNSFRDNYLGVTFDLSNVMFMTTANVLDTVQP
                     ALRDRMEVIRLAGYTEEEKVEIARRHLIPKQIEENGITARNLQLSKPALAGIVKQYTQ
                     EAGLRQFEREIGTICRKVARRIAEGRSEIVRVTQKNIHEFLGAPKFLPEEVLKKDQIG
                     VATGLAWTAAGGDVLFFEALRMRGKGGLVLTGQLGEVMRESAQAAYSYAKSRARELGI
                     PEEDFEKFDIHLHVPEGAIPKDGPSAGITLATAMVSVLSQRPIRKDVAMTGEITLRGN
                     VLPVGGVKEKVLAARRARVSKIILPAQNRRDMDEIPKELLGEIQFVFAEHVREVFEHA
                     LKEPVRAPAAPPVPRSTRPPQAAPQPSA"
     gene            25652..27409
                     /locus_tag="VF240_01480"
     CDS             25652..27409
                     /locus_tag="VF240_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7173941.1"
                     /translation="MNNTSLPRLIAGAALSAAFVFLPVASLRAQVAQQSYLRHTTSDA
                     DREDPRIQAVIARAESHFKLGELNLKDGKREQARDEFDKAVDAVLESGLDVRAYPRLQ
                     QYYLQLVERVYRVEVPTQDQQQRTVVAETQGMPASDGGQQQAAQVVQVGFVDQKFEPS
                     PLDEMSRLVLTEKEKEVTVGQVAEAEEEIKNSLDFQFKPHPLVLQFINYYQGRGRATM
                     ETGLRRSGQFTPMAREIFRKHGIPEDVVWLGQVESAWRPNARSWAAASGLWQFIPSTG
                     SRFGLRQTAFVDERNSFEKATEASAKYLKWLYNRYHNWELALGAYNTGEGNIDRAIAR
                     AGTKDFWAIYPYIAKETRDYVPNILAVILISKNPQKYGFRDIQRMPRIDRQYDTISVP
                     SATSLHLLASLTDSSSDYLRSINPELRRDTTPRGESYHVRVPAGKGKVAVDLLKRIPM
                     ERREQLARVVTVSPGEDLQAAASRAGVSVSQLQQWNGGADLSKGGKVVVPAGSVQRTS
                     KIYERPRGSAVAGSTIKSVTAKGGETLAQLAAQHNASVEEVAKYNGIAANAQLTRGQR
                     ISIPTAAGAAPTARAPRRR"
     gene            27652..29217
                     /locus_tag="VF240_01485"
     CDS             27652..29217
                     /locus_tag="VF240_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX7173942.1"
                     /translation="MLFRTQSAAVYGIDADLVDIEVDLVPARGESDSPSSVTIVGLPD
                     LAVRESRERIRAAVNNSGFFFPFHKTTINLAPADVRKEGASFDLPIALGVLGANGDLG
                     DAEGLTDMLSVGELSLDGRVRSVRGALSVAVRAREAGIRCLLLPEENAKEAAVVAGVD
                     VYAVTDLRGAVELVAALRGPTPPAPLRIDPAEVLAPARTSGVDFREVRGQLAVKRALE
                     VASAGAHNILLVGPPGSGKTMLAKRLPTILPPLEFEAALELTKIHSVAGLTGRSGLVT
                     ERPFRSPHHTISDAGLIGGGAIPRPGEVSLAHHGVLFLDELPEFDRSVLEVLRQPLED
                     QRVTISRAAMTLTFPASFMLAAAMNPCPCGFWNDPTRECRCTPLQIQRYVGRISGPLL
                     DRIDIHIDVPAVKFKELSGASTHEGESSEEIRERVIHARERQRERLQGEGIFSNAQMA
                     TRLIRKYCRIDADSERMLEQAMTRLGLSARAYDRILKVSRTIADLDGAADIRPAYVAE
                     AVGYRSLDRTYWA"
     gene            29758..32514
                     /locus_tag="VF240_01490"
     CDS             29758..32514
                     /locus_tag="VF240_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX7173943.1"
                     /translation="MRKGLLTGLIGVALVAASMFPSMNSDAARAPQGGEISNNRSQRA
                     GLPNHDIRLAGRGEFTDVDVSPVAGARRAVPNAGAAVQARAAAVENFRAGLRPEQASN
                     LRAEVNEAGALKNIFIDGASLSGPQSDAPDQIARGFLKREAALFGLSHAAVDELKLEK
                     EDNDAGTTFLDYAQTVGGLKVFEGNVKVAVNRNGEVLSVREGFIVAGQRGRLRPTLTE
                     TEGIAKAFEHAGRAVLPSALAETRGRASQSDNAAFANPLGAGFEEVLSELNVVRVGDA
                     ARLAWHVYAEVGPEEWYELLVDAHSGELLLRHNLYVHQAQGTVYTEAPDKGARVLVSF
                     VGDTVINTSAGWMGTSTVTTGNNVEAYLDRDANNSPDTTSGNGLSNGHASAANQNFTF
                     PFDTAVSPTTATNQAAVVTNLFYYNNIMHDFSYNLGFTETSRNFQTNNYGRGGLGADS
                     VRAEAQDGSGTNNANFATPPDGQRPRMQQYIFTTPNPDRDSSMDGDVVFHEYGHGISN
                     RLIGNGSTALQGTQSGAMGEGWSDYWAITINGDGAVGEYSTGNPNGIRRAPYTVPANT
                     VHDSYADVGVGGFQVHRDGEVWAATLWDLRTQLGATITDRIVLNGMKFTPTRPSFLNA
                     RDGILQADQNLNGGANRCAIWTVFARHGMGVSAVGNDGTTHTAASDLPSDCGGTCSYS
                     ISPTSASVAAGSGTGTVTVTAGVGCAWTAVSNATFITITGGASGSGNGTVSYSVAANT
                     ATTSRAGTLTIAGLTFTVNQAGTGGGGTNVIVNPGFESGTTPWVISSQVTRSTGAYPH
                     SGVAYMIINGVNSTSGTLYQTVTIPAGGANLNFWLNITTSEAAGAAIYDRLFIEVRNT
                     SGTLLGTLATFSNQNSGTAGVYVLRGPYNLNSWAGQTVRIQFRGTNDITLPTSFRIDD
                     VSLQ"
BASE COUNT         5792 a        10485 c        10785 g         5518 t
ORIGIN      
        1 taaggacaga ggtcagagtt cagaggtcag aggttagtaa aagcaagatg aagaggccgg
       61 aggttgtgtt tcggacacta acctccggcc tcttggtttt taactggcct ctaacctctg
      121 acctctgacc tctctgcctt acggctcgtc tgagcctttt ttgccactct tcttttgcgg
      181 cgcatcggtg tcgccgcgtg ggcgcggttg ggtggcgggc ctggggggcg gggcgtcgtc
      241 cttcttgtcg tcgcgtgggg cggccggctc ggacttgctc ggagttgcgt ccgccggggc
      301 ggacgatttc gtctcgccgc cgatggtggg cggcagcgtc aactgctcaa gcttcgggtc
      361 ggtgacggtc gcgggcagcg acgagtcgtc gtcgccgctc ttgtacgacg agcgcagagc
      421 catctgcgac tcgcgctcct cggccatctc acgctgccgc tggaggtaca gctcgcggat
      481 gtcctcgggc atcttcggcg ccttggcgaa agtctccttc ggcttgtcct tgaggaactc
      541 cttcatgtaa tcgacccaga tcgggagcgc gccgtgcccg cccgtgaagc ccgcgcccag
      601 agattgcttg cgctcggggt agcccatcca gacacccgta acgtaggtcg gcgtgtagcc
      661 gatgaaccac acgtccgtgt ggtcgttgac ggtgccggtc ttgccggcca cctgatggcc
      721 gacggacgac gccgcggccg ccgtcccgcc cccggaagtg acgccgcgca tcatctcggc
      781 catcgacatc gcgacgtact cgttcatcac cttgaaggtc gtcttctccc actcttccag
      841 gaccgtgccg tcgcggtcgg tgacgcgacg gatgaggtgc ttctcatggc ggatgccctt
      901 attggcgaag gccgagtagg ccgagaccat ttcgtcgagg ggcacctcgg tggcgccgag
      961 tgcggaaggg agatagggcg ccatcggcga ggtgatgccg aagcggcgga ccatctgcgc
     1021 gcccgtctgg atgccgacca tctgaagcag gtggacggcc gcgagattca gactcttggc
     1081 cagcgccgtc ttcatcggga cgttgccgtg gctcaagctt ccgtcgtagt tgtgcggctg
     1141 ccagcccgag gagcggtcac ggaagggcgc gcccgagact atcgagtcgg gcgtcagacc
     1201 ccactcgacg gccgcggcgt agacgaaggg cttgaagcag gagccggtct ggcggtaagc
     1261 ctgcgtggcg gtgttgaact tgttgaggtt gaagtcgtag ccgccgatca tcgagacgat
     1321 ctcgccgtcc ttggcgttga tcgtcatcat cgcgcccgag acttcgggca cctgcgagag
     1381 ttcgatcttg agcgtgcgag cgttctcgtc aacctccttg attttgaact cggcgagatc
     1441 gcccagcttg aactggtctt tcggctggcg tgcagaccac cccatgtcct tcgccgtgac
     1501 gacggcgcgg taggggccga acctcacggc cgcctcgttc ttcgcacggt tcacctcgac
     1561 gacgaggccc atcagatact cgtccacctc gaacttctcg acgtaccagt ccgggtggcg
     1621 ataggcggcg agctgctgcg gcgtgggcgc ggccccgttc gtcgtcgggc cagcggcggt
     1681 tccgttgcca ttcccgttgc cgtttccgtt cgtgccctca ccggcgatgt tccgaaactc
     1741 ggagcgccag ccgcgcccgc ggtcgtattt gcgcaggccc ttacgcagcg tgtcgtaggc
     1801 gagcttctgc gcctcgacgt tgatggtcga gtagaccgtc agcccgctct gcgcgacacg
     1861 cgtcgtgtac ttgtcttcga gatactggcg aatctcctcc acgggatagt tgaagatgga
     1921 ggagcgcgtc tggacggtgc cgtgggccgt gccggcgagc ttgatcggtt tggccttcgc
     1981 ggcctgtgcg tccgacgccg agacgaactc gttgcgggcc atcaggtcga ggacgaggtc
     2041 gcgccgctcc ttggccgtct ccatgctgac cgtgggcgag tactgcgagg gcgcgcgggg
     2101 gatgccggcg aggagggccg cctcttcaag ggtcaagtcc ttcgcgggct tgttgaagta
     2161 ggtgcgcgcg gcggcctcga agccgtaggc gttggcgccg aggaagatgt ggttgacgta
     2221 catctccatg atctgctgct tggtgtaata gcgctcgatc tggagggcga cgacccactc
     2281 ggtaatcttg cgctcgtagg tctgcttgcg cgacaggaag aggtttttcg agagctgttg
     2341 cgtcagggtc gagccgccct cgcggctgcc cgtcgtgatg ttcttccagg tggcgccggc
     2401 gatgcggacg gggtcgatgc cgacgtggtc gtagaaacgc acgtcctcga cggagaggat
     2461 cgccttgtga atgagcggcg gaatctcctc gtacttgagc gggatgcgac gttccagcgc
     2521 gaactcgccg atgacggtct cgccgtcgtc ggcgtagatg cgcgtaatca ggctcggctt
     2581 gtaggtggcg agggatttga cctcctcggc ggccttcgag tagttgagct gataggcgag
     2641 cacgatgccg gtcaatccgc cggcggtgat ggccagaaga atcacggccg gcagccagaa
     2701 gcggctcagg atgccgcgac ggcgcgcggg cggcggcggc atgcgtcgtc gcggggccgg
     2761 cgcgctggtg tctgtgatcg agagcgattt cttagaggac atgaacaaaa atccctcaag
     2821 tgaaacgagc gtgtatctgc tgacgtgtct ccaaaggctt tgatgctcgc tgagaccggg
     2881 gtagcgcggt cgagaaatta taacgcacgc gcgggcgggc gggcgaaccg tttcggcggg
     2941 cagtgtgggg agccacaccg ccgggccgtg gctggccggc ggggcgggca cccggccggc
     3001 ggcgcgcagc atctaaatct ttgtcgcgcc ggtgtttgtc cgaacggcgc ggcggcggtt
     3061 ataattcggt ggggcttcgc aagaagcctt tacgggggcg aaaggtttcg acaggggtcg
     3121 ggatcgtcgc acggatgcat gccgggctta ctctatgcgg ctcgttaaaa agcatgggga
     3181 aactcaaatg ccaacaacaa cttggctctc gctgcttaat taaccttaac cagtgagcgc
     3241 ctcgccgcaa gtcgctcaag cgcgcggagt gaggtgtcac ttatttgagc tggcccgaca
     3301 catgtgctca ggtgtggtgg gcgagaaaaa tctgggctag ccgccggcgg ggtcttcgac
     3361 gcttcactta cccgccggca gcgaacgcgc ccgagtgaga cgggtagcgg agaagcgtct
     3421 aagcatgtag attcctgcgg cgtcggcctt tggacccgag ttcgattctc ggcgcctcca
     3481 tcgttacgcg aaaaggcggt ggctcttttg aaagagtcat cgcctttttc tctgtcgaga
     3541 ctttagagtt cgttgcaaga cttattcggc gttcgtgacg agtgtagggg cagagcttgt
     3601 ccctgcccct acaggtctga catgcgttgt tagttggaag ccgccgaagc cgccgtggct
     3661 ttctttcgct ggttcgccag aacgcccgag tccacggtct cgccggcgcg cgtgatgacc
     3721 tggaagtgca tctggtcgcc ggcgatttcg aggagcatga aggacatgtc tttgtcgaac
     3781 gccttctcgg tgagcggcga gttgtccttc acgtcgccgg ggcggagttt gccgcccgcg
     3841 cccgagatga aatagtagat acctttttgc ggcttgagcc gctcgtagaa gtgctcgtgg
     3901 ccggcaaaga cggcgttgac cccatacttc aaaaatatcg gctcgacgat ctcacgcacc
     3961 cccgtcgagg agccatgctt gccgcccgaa gaataaggcg ggtgatggaa gaaggcgatc
     4021 ttccattccg aggcgtcctt cgccagctcc tcttcgagcc acttcagctg acgcttgtcc
     4081 atgtagttgc tgttgagcgc gtagaaggcg atgttgccct tctggagacg gtaatattcc
     4141 tcgcccttca tgttgaagtg ctcgtagaaa cgctggttcg actcgtcatg attcccgagc
     4201 gaggcgtaga acatcacctt gtcgtcgagc agacgcttgt agatgtcctc gaacttcagc
     4261 ttgaagtccg cgggcttctc cccgccgtac atgttgtcgc ccatcagcag gacgaactcg
     4321 aaggggaagg cttcgcggta gcggagcatg acgtcggcca actccttctg ctgctccgtg
     4381 cccgtccccg tatcgccgac gaccatgaag cggacggaac cgtccttcaa aggcagcgtg
     4441 agcttgacgg gcgaggcggc acgcgtgctg ccgaccggct cgccggcggg tgcgttctgg
     4501 ccgagggcgt ctggcgcgag gccgccgccg agggcgagcg ctgcggcggt catcaacgaa
     4561 gcgagaaact gtctcataat cttttctccc atgattgcga cggactacgg gacggcgttg
     4621 agcatgtagt caacgatcag accgttctct ctatcgacga gcagcaggtt ccggccgacg
     4681 aaacgatacc tcacctgctt gggcaactgc gggagcttca gcaacagcgt cggcggcatc
     4741 tccaccaact ccttcgcctc cggataggtg tagttgacgc gcaggggtac gcccttcgtg
     4801 tccgcctcca gaaccgtctc gcgcagcttc tgccgctcct tgccctgaaa ctctttgcga
     4861 atggtggcgc ggatgtagcc ggcgacatct ggcttgaacg cgtcgccggg tttggcgccg
     4921 atgcgagcgg cccgcaccat ctcctcgaag gccgtcttgt gcgcctgtat ctgctcgggc
     4981 gtgctctcct tgggcagctt cggcagcttc tcctccatcc cctcgcgcat cacggcgtat
     5041 tccttcacgc gcccctcgaa ggccgcgacc cccttcctat acttgacggg aatctcgggc
     5101 ggggcgtcgt gaacctgctg cgcgaaagca gaccgggcgg acgaagcggg cgagaaactt
     5161 aaggcgagca ccacggccac acccgtaacg aatccgatac gatgatttaa tttgcgtggc
     5221 atcggcagat aaaagtgcaa acgggatgcc agcaaacggc ccggtccgac acttcccacc
     5281 gccccgttaa caagttggaa atacaggcgg atacgacggc aagcgccggg gaggcgcgct
     5341 ctcccgagga agggggcgaa cgcttgtaga acattcaggt cgcgcttgag tacacaaccg
     5401 aaggaaggca aaagtaaaaa ggcaaaaggc aaaagtgaag aggcgacggg ccttctccct
     5461 ttttcctttt gccttctgtg gccttaacgg ccctcgatga tgtcgcccga gtcgatctgc
     5521 ccgcgccgca cctcgacgga cttcgagccg aggtgacggc ggaagccgcg gaagtaggag
     5581 ggcaaaccct cgacggtctt gtagcccgcg ccgtcgcgca gctcgtagtg caggtgcggg
     5641 aacagggaac tgcccgaggc gccgacctcg gcaatctgct ggccgcgcgt gaccgcgtcg
     5701 cctcgcttca ccttcacgct gccgcgcttg aggtgcatga agatgctgaa ctcgccgttg
     5761 ccgtggtcga tgacgacgta gttgcccgcc ggggccagct cgcgcgtcat gatctcggcc
     5821 tcgtcgaagc gccggtcgtc ggccatgcgg tcgtaggcgt cggcgacgcg gcccgcgccc
     5881 gcggccagca cgggacggcc gaaggcgaac caactctcgt tgcgctccgc ggggccgtcg
     5941 cgcatctcgc cgcgctcgtt gacgtggacg aagtcgtagc cgaagcgtcc ggcgttcgtc
     6001 ctgaagccga gctgttggag gaaggggtgg aggtagtccc agcgccggtg atgcgagtag
     6061 aagtcgtgcc cgtcccagac gagcacgcgg cccacgagcg gcagcacgag atcggtcttc
     6121 gtctcgtagg cgacgggcga cacgtccgtt gtggcctcct gctttttctt cccgtccttc
     6181 gacgcgaggg agacacggaa gcgcaggcgt gtgaggtcaa cgccgtggtc gaagaagtag
     6241 agcggattga agacgagtat ggtctgcttc ggctcgacct cgcggacggg gacgacgtgg
     6301 atgctcggcc gtccgccgtt gtcgttgacg aacttgcgca gcacgagcgc gccgcgcgcg
     6361 tcgaagaccg agacctcgat ctcgttgatc gtgagcggct cgtcggtcag gttctcgacg
     6421 gcgaggtcgc agttgacgag ctgccccgcc gcgctgcgct cgacgatgac ccggcgcggg
     6481 tgtgcgctga cgcgaaccgc cccggagtcg ccctgtccgc ctcgcgcggc agttgagagc
     6541 atgagcacga ggaggagaag gaacgtgatg cgtattttca tgtgacgtgt gtcgaatcta
     6601 cttcgtggcg actcaggcgg gcgccgccgc cgagagtttg ttgtgatcgg agccggcggc
     6661 cttcgactct ttaacacttt tcgccacgtc ggcccgttgc gctgcgccgc cccgccgttg
     6721 ccacgggacg aacgagaggc tcaagaggag ccattgcagc atcgtgatga cgacgccgat
     6781 cttgtcagtc gtgccgtgcg gctcgccgga gcgaagcaag aagagttgga ggggaagcag
     6841 ggagatcgtg gcgatgagaa gggctgtgtt gcgccccgtc cacggcgggg cctcgcgccg
     6901 ctcgcgccgc cgcaggtcgc gcacgaccca cgcgagagac gcgagcgcga tggagccgaa
     6961 gatgagcggc ccgacccacg cgagcgatat gtcgatgccc atcgtgtcgc cacggaagcg
     7021 atactcatcg gggaaacgca cggcgttata cgtggcctcg acggcggccg ggacgttcca
     7081 cagcaggagc atccagtgga agggcggccg cgcgccgcgc cagcccgtgt agagcatcgt
     7141 gacgccgaag gcggccacga gcggcagcac ccagtagtcg ccgcgcaggc cgcgcccgcc
     7201 gaagttgatg ccccagagcg tccagcccca actgtacgtg tcgccgtcca acaagccgcg
     7261 caccgtcggg agccagaagg taagcatggt aaacatcgtg agggccagaa tgagccgcag
     7321 aagtcggtcc ggtttccact ccatgagtcg agtcctctag gtaggtcgtt cgctgaatgt
     7381 ttatggttaa actcgccccg agaccttgtt caccctcttt gtctgtcatt ccaatacgga
     7441 agaagacggg aaatgttcac gcgctttccc gccttcgtcg cgagccggcg cgagccggcc
     7501 acggcgtgtg ataccatctg cccgacgcgc ccgtgcgcgt ctcgcttgtg agacctccga
     7561 cgaagcgccg atgaacgatc tgccaatcgg aatttttgac agcggcgtgg gcggtctgac
     7621 cgtctaccgc gcgctgcacg accgactgcc cgaggagcgt ttcgtctacc tcggcgacac
     7681 ggcgcgcgtg ccctacggga cgaagtcatt ggcgaccgtg gaacgctacg ccattgagaa
     7741 cgcgcgcttt ctcgaagcac acggcatcaa gcttttagtc gtcgcctgca acacggcctc
     7801 ggcgctggcg ctgcccgcca ttcgggaaag cgtcggcgtg agcgtggtcg ggatggtggg
     7861 gccgggggcg cgcgccgtcg tgcgcgctgc ggcgggccgc cggatcggcg tcatcgccac
     7921 cgagtccacc atcaggagcg gcgcctacgc acgcgagatc gcacgcctcg cgcccggcgc
     7981 ggaagtggtc gagcgcgcgt gcccgctctt cgtgtcgctc gctgaagagg ggtgggccga
     8041 gacggaggtg gcgcgcacgg tcgccgccga atacctgcaa gacctgcggg gcgggagcgt
     8101 cggcgcgctc gtgctcggct gcacacacta tccgatcctg cgccgcgtga ttcaggagac
     8161 gatgggcgaa gacgtgcgcc tcgtcgattc gggcgaggcg gcttcggagg ctgtggctga
     8221 ggtgctggac gcggagggct tgcgccggcg cgagtacgac acgcacacgg ggcggctctt
     8281 gaaggagcgc gcgcgcgtcg gccgactgtg cgacgacctc gatcatttct acgtgacgga
     8341 cgcggcggag cgtttcgcgc gcgtcgccga aaggtttctg gggacggcgc cgcgagttct
     8401 cgaagccgtc gaagtctggg ggacggatgg actcaacacc gcggagcgaa cagttcaaac
     8461 gtagcgacgg gcggcgccct gaagagttac gaagcgtccg catcacgccg cacttcatgc
     8521 cttatgccga aggctcggcg ctcatcgaga tggggcagac gcgcgtcatc tgcgcggcca
     8581 cgctcgaaga gcgcgtgcca ttcttcaagc gcaactcggg gcagggctgg ctcaccgccg
     8641 agtatgcaat gctgccgcgc gccacgcagc agcgcacgca acgcgagacc ggtcggggcg
     8701 ggccctcggg ccggacgcac gaaatccagc gtctcatcgg ccgctccttg cgcgccgtct
     8761 gcgacctcgg cgctttgggc gagcgcacgc tcaccgtcga ttgcgacgtg ctccaggccg
     8821 acggcggcac gcgcaccgcc gcgatcaccg gcgcttacgt cgccttcgtc ctcgcctgcc
     8881 gccggctcaa gaatgcgggc cagatcgcgc gcttgcccat ctcgggcgag atggccgccg
     8941 tcagcgtcgg gatagtcggc ggcgcggccc tgctcgacct caagtacgac gaagactcgc
     9001 gcgccggtgt ggacatgaac gtcgtcggca cgggcgacgg ccgcttcatc gagatacagg
     9061 gcacggccga gggcgagccg ttcagccgcg atcagatgga cgaactcctc gcactcgccg
     9121 gcgccggcct cacgcaactc ttcgccgcgc agcgcgccgc gctcgaagcg ccgcagccct
     9181 gagccgcgaa aagttgtctt agcgaaccac ggccgagccg ctcggacagt tgccagcggc
     9241 tcacgaagcg ggcaacattt gggcgcggcc ggcgcatctg attgaacagt caggcgggaa
     9301 aaagatgaat cccgaacccc aacaattacc gacacgagac aggagttgaa tccccacata
     9361 tgagacgcaa catgtttttg ctggcctgcg tgttgacgct cggcgtgtgc ggggcgcaag
     9421 cgcgggcgca ggaagtcttc gccgacccga acgtcgaata cacgctggag ttgccgacgc
     9481 cgacgtggaa gaccacgagc cgggccgacg gcgtctatca gcgcgtcgag ttcgtcaacg
     9541 gcgaccggat ggacggctac ctgcgcatcc gcaaggaagc catcaatcac gatgagacgc
     9601 ccgccgcgat tgccgcccgc cacgccgacc tcaagctccg ctacaagtcc ggctacgtcg
     9661 gcggcggcaa gccggagtcg ttcgccgggc gcctgaaggg cgtgacgatg ggctacgagt
     9721 acaccgaggc cgggaagcag atggccggcc gcgtctacta cctccaggcc gacgaccaca
     9781 ccgtctacgc gctccacttc acgggcctgc gcgaaaagct ccagcgcgcc cgcaaccaga
     9841 cggacagcat cgcccgcagc ttccgtctca aacagtaact cctgagacag aagcgacgca
     9901 cacgaatcag ccgcccgcgc gctttgccag cgccgggcgg ctgaactatt attcatcgac
     9961 tcaataaatc agtaccgcga gcggaagcct cgcgggtgcg gcctacgatg ggaggcccgc
    10021 accgctcaag cgagacccgc tcgcggtact gatttctcat cccgatgaaa ctacaactgg
    10081 ctccggggct tgccgtcggc gagtcgaaga ttcacggccg cggctgtttc gccgccgccc
    10141 cgttcccgaa ggggcgcaag atcgccgagt acgcgggcga gctgatctcg ggccgtgagg
    10201 cggagcggcg gatgcgcggg cgcgaagttc accgcgtctg cccactcgaa acgggccgct
    10261 acctcgacgg cgcacgcggc ggcaacggca cccactacat caaccactcc tgcacgccca
    10321 acgcctacat gcggctcacg cgcggccaca tcctcttcat ggccctgcgc gacatccgcc
    10381 ccggcgaaga gatcaccatc gactacttct ccacccacca ccccgacacc aagcgctgcc
    10441 actgcaaagc gccgacgtgt cgcggcacaa tcaacaaaag gcgtgataag tgataagtga
    10501 tgagtgataa gaaagacaaa gaccgtctgc tctttcttat cactcatcac ttatcactta
    10561 tcacgccttc acagtctcgg aagcgtgaag ctgaacttcg agccgcggcc cacttcggat
    10621 tcgagtccga cgcggccgcc gtgctgttcg acgacttcgc ggacgaagga gaggccgagt
    10681 cccgccgagt cttcctcctt gtcctgcccg tcgcgcacca cgcggtagaa cttctcccag
    10741 acgcgatcca cggcatcggg cgggatgccg tagccgcaat cctctacggt gacgcgcacg
    10801 gcctcggatt cgagcgccgt cgtgagcgtg acggtggtgc gctcggggct gtagcggatg
    10861 gcgttgtcca cgatgttcat cacggcctgc gtgacgagcg cgcggtcggc ggccacgggc
    10921 gggagcttgc ccgagccgtc tgagccgacg agtcggatgc gtttacgctt ggcgacgggc
    10981 tgcatgcaga ggactgtctc gcgcaccacg tcgctcagca tcagcggaat cttcagaacc
    11041 tcctgtcgat ccttctgctc cagcttcgtc accgacagga aggtgttgat catccgcacg
    11101 agccgctgcg actcgctgtg gatgatgccg aggaattcgc gcgccgactc gggcagagta
    11161 tcgtcggtcg cgagcagctc ggcgaagccg ttgatggagg tgatgggcgt gcgcaactcg
    11221 tgcgacatga gcgagaccat gtcgctcttg agcttttcgt actccttcat ctccgtcacg
    11281 tcgcgcacgg tcacggcgag gcacaagggg cgggcggccg gtgagccgtc ctcgcccgcg
    11341 tcggtgacga gggcgatgcg gaggctgagc gtctgaccgc gctcggagaa ggggagttcg
    11401 cactcatagg cgctgccgct ctggaggacg cggcggacgg cgatggaagg ctcgtcgaag
    11461 acgccggcgc ggaagcggtc gagcaggccg aagaggtcga gcgtgtcgca gtcgccttcc
    11521 gccatgcgcg cggcctcgcg catcggggcg ttgacgtagg cgatggtgcc gtcgaggtag
    11581 gcgacggcgt ggccgtcgtg cgactcggag agcgcgtgcg cggcgaacct ctgctcggtg
    11641 agaaggccgc tcagcatgcc gaaggattcg agccggcgct cggcggcgcg caccgagtag
    11701 aaggtttcgg acggcgcacc gacggagtga gcgcgcgcct cgtcgcgctg gaggtcgcgg
    11761 gcgagctgcg cggccacgtt ggcgaggagc ggccggtcgg tctcgtcgaa cccgccgcgc
    11821 aggcggatga ggagcgcccc gacggcgcgg ccctcgtggc gcaggggcac gacgacgctc
    11881 gtcgtcgtgt gcgcgtcgtc tctgtgcgcg tgtgcatcac tgtcgttgcc tgtcgcttgg
    11941 gtttgcggtt ggacgaccat ctggccggag cgtatggcgc gctcgcagag tcggatgccc
    12001 tcgcgccagg ccgtgttgcg gttcgatgcg gcagtcgccg cgccgccggc gtcggggcgc
    12061 atgtaggccg cgggcgtcgg cctgccgtcc tcgtcaagac agcagacgac cgcttcgtcg
    12121 agcggcagca cggtggcgag cagacgcagt ccactctgca tccgcgcacg cgcctcgcgg
    12181 ccttcgagcg cggaagagcg cgaggccaca cgccgcacgc tccccgagag cgccacgtcc
    12241 acgatccaca gtcgccatgc ctgcacggcg aggaaggcgc cgagcgtggc gagcgcggcc
    12301 ggagcgaagg cgatgtcgat gttcaggaaa taagaggcga ggtagctggc cgcgcacatc
    12361 agcgcgaggg cgcaggccag gaggagtgag gtgcggcgcg cgccgacgat ctccacgagg
    12421 aacccggcgc acgcgcccgc cgcgccgatg agcagcgcgg ccacccacca ctgcggcgca
    12481 atcgcggggg ccgcgtcgcc gctcagcagg aacatcccgc cgatgaggac aagcgccccg
    12541 ctgctccaca aagcgaaggc gaaggcggag acggcgagcg agagcgcgac cgagacgaga
    12601 cgtgacttga gcgtaggaac gacgaaatgt ttcaaggcga tgtgttcgga ggacgaggtc
    12661 ggaggcggga cccggaaagg ctcggccgga agtgtacgcc ggccctgtca aaaactatac
    12721 aacgcggggc gtgactaaaa caagagttgg ttttgcgggg gcgacttcca gtgaattagt
    12781 tgagtgtgaa gtaactggac tcaccgcaga ggcgcagagg acgcagagga aacgcggagg
    12841 cttgagtttc agaattcact caagacaggc aggcacaggc aactgcgtcg agcaactcgg
    12901 cctcttcgtc ttccgcgacg gttcgcaggt cgagcgccac gcgcccatcg agaatgcgcg
    12961 cgatgacggg cgggcgcgcg tggcgcaggg cctcttcgag cgcgtcggcg gagagcaccg
    13021 ggtgggagag cgcgacgagc gccgtgggcg ggtgcgtggt cggcgcggag ccgccgccga
    13081 cggccgagcg cccctcgacg acctcgacgc ggagcgcgcc cgcctcagac ctcgcgcgca
    13141 gaccttcggc gaaggcgcgg gcgcgcacct cgatctcctc gcgtgtggcg gagagcatcc
    13201 gtagcgccgg cacttcggac gtggccgtgc cgcgtcggta ggcttcgagc gtggcttcga
    13261 gcgcggcgag ggccagcttg tcggcgcgca gcgcgcggta gagcgagtgc cggcgcaggc
    13321 gttcgaccac gtctccgcgg cccacgatga ggcccgcctg cgccgcgccc agaagcttgt
    13381 cgccgctgaa ggtcacaacg tcggcgccgc cctcgattga gtcgcggatg accggctcgt
    13441 cgccgagtcc gtagggcgtg aggtcgatga gcgcgccgga gccggcgtct tcatagagcg
    13501 ggagaccggc gcggtgcgcg agttcggcga gttcggcgag cgagggcgcg gccgtgaagc
    13561 cgacgatgcg gtagttcgag ggatgaacgc ggaggatgag acgcgtgcgc tccgtgatgg
    13621 cgcgctcgta gtcggagagg cgcgtgcggt tcgtcgtccc gacctcgatc atcgtggtgg
    13681 cagactgcgc catcacgtcg gggacgcgga agtcgccgcc gatctcgacc agctcgccgc
    13741 gcgagacgat ggtctcgccg ccgcgcgcca gcaccgagag cacgagcagg gcggcggcgg
    13801 cgcagttgtt gacgacgagc gcgtcctcgg cgccggtcaa ctccgcgagc agtttctcgg
    13861 cgcgcgcccc gcgtcgcccg cgctcgccct tcgcgagatc gtattcgagc gtgcaatagc
    13921 tcgcggcctc acgcgcgacc gccgcccgcg cggcctccga gagcggcgcg cgtcccaaat
    13981 tcgtgtgcag gacgacgccc gtcgcgttga tgacgcggcg cacccccgcc aatccttcgc
    14041 gcgccgcctc acgttgcagc ctgcgcgcgg cctccgctaa aagttcatcg cgcgccgccg
    14101 cgccgttgcc atccgccccg cgcgccgaca actccgcccg caactcctcc gtgacggagc
    14161 gcgccagcgc cgtcagacgc accgcgcccg tccgctcgcg cagctcctgc gcctcgggcg
    14221 ttcgcaaaag cgcgtccacc gacggcaggg cgcgcaagac gttggcagtg gtttcgtcgg
    14281 gcatatggaa gctgttagct gttagctgaa agctgttctt tgtcgatggt tggtagggag
    14341 ttgtcagtca tttccgtttg ccggcttgta cgtttcattg aaggaagcga ggcgcgctag
    14401 acgaacaact gactgttgac cactgacaaa aaagctgaaa gctgatagct catagctgat
    14461 agcttatcat aatcgttgac acccccaccc gccggctcat agactcgaag gggtgcccgg
    14521 caacgacccg gcgcggtgcg cgaggagagg tgaaacagtt ggctaaaggt ggaaacaagt
    14581 ttacgcggca ggcgcaggag cgggagaaga ggtcggtcgt cgcgcgcgac gcgcagccga
    14641 ccgtcgatga acagctcacg cgactcgatg acgatgtccg ccgcctcaag gtcgagttcg
    14701 acatcttttt caacggcggg gccaagcgac cgccctacga cacgaagggg cgcgtcgaga
    14761 cgctcatcaa gcgtctggcc gacgaccgca cgctcaccta cgcgcaacgc taccgctaca
    14821 actccctggt ggcgcgtcac acgtccttcc tccaactctg gcgccgcacc atgcaggggc
    14881 gcgaggaggg gcgcgaccct ctttcagccg cccgcgccgc cacgcacgcc aagccggagc
    14941 cgccggcgag cgtggccgcg ccgaacacct tcgtctgcgc cgatccgcac acggacgtgc
    15001 cgaccgtcaa aaggctcttc gaggcgctcg tcgaggccaa gcggcagtgc ggcgagggaa
    15061 ccgacgacct ctcgttcccg cgcttccatc gcatgatcgc cgccaagacc gactccatca
    15121 aggaaaagct cgactgcgag cgcgtgcagt tctcggtcag cgtcaaggac gggcgcgtga
    15181 acttcaaggc gaaggcggag aagtaaaaaa aagtaagcag taagcagtga gcggtgagca
    15241 gtagagacta agtctcgttt ctactgctca ccgctcactg cttactgctt actgtctact
    15301 gctttcttga gtgccttgct tcgggctgcg tgtgccggct tcggaaaaac cggaggcggt
    15361 cagtggcctt caacgaaagc tggcgtgtga ggggagactc acaccttcat cactttctcg
    15421 gcctgcgggc ctttcggacc ctgcgtgacc tcgaactcga cctcctgacc ctcttcgagg
    15481 gtcttgaagc cgtcgccctg aatggcgctg tagtggacga agatgtcgga agagccgggc
    15541 tgctcgatga agccgtagcc cttcgcgttg ttgaaccact taaccttacc ggtaattcta
    15601 gacatagcta acgaatcctc ctaacgattc ggtgaacggg ccgaagggaa gagagaaacc
    15661 ccactcgccc ggattgttaa cgcctgcgag ccggcgcggc cctgagcctt tgcgcgcgcc
    15721 aactaactca aactaaaaga cccgcgcccg gtgagaccga gaggctcacc aagtacgggt
    15781 ctacaactga cggggaaact gctccgaact cattgcggta atgcctgcaa gtctaacgcg
    15841 gggacgaggt taaacaacaa gcccgtccgc aaacgaacgc gcatactaag cgaaagcagg
    15901 caaaagtgtc aagcacaaaa tgcgcgtaaa accgccctgc tcccggcgcg actccttatg
    15961 aaatctcagg gcatcggcag ataacgcgcg gggctgacgg ccgactcgcc catgcggact
    16021 tcgtaatgaa ggtgtggccc cgtcgaacgg cccgtggagc cgacgcgccc caacgcgtcg
    16081 ccgcgcttaa tcggttgtcc cagctccact tcaatcttcg agaggtggcc gtaggcgctc
    16141 gaaagaccgt cgccgtgatc gacgatgacg atcaggccgt agccgttttt gacgccggcg
    16201 aagctgatcg tcccgttgcc ggtggcgacc acgggcgtgc cccatgcggt ggcgatgtcg
    16261 aggccatcgt ggaactcggc cgagcctccg ccgaacgggt tgccgcgcac cccgaagtcg
    16321 tccgtgatct cgccgccgac cggccagatc gaagggatgc gctcaagctc aagcttgcgc
    16381 cggagcgcgt cctcgtaggc gcgcaactgc ccttcgagct gcgaagcgcg cacctcgacg
    16441 gccgtcacgg tcgcctcgtc ggcgagcacc tgcgggccgc ccgcgccgtg cgggctggtg
    16501 ccctcttcgg ccgggacgcc ggaaatctcg gagagtcggc gcgcgtccgt ctcgatggcg
    16561 tcgatgcgcg tcttcagctc gtcgagtttc tggcgctggc gctcgttctc cagacgcagg
    16621 cggttgtact cgcgctcgat gcggaggtgt gcggtctgcc gcgcgaggcc gtagacgccg
    16681 tagagcgcgg cgcagacgac cagcgtggcc gcgcaggccg agacctttac ccagcgttta
    16741 tgtatggaaa tccggcggat gccggcgcgg gagcgcgaag tgtgggcgac aataaaagcg
    16801 tagatacgct cgtccttcat catcggagtg tgttccttgg ggctccgtcc gggcgggaat
    16861 taacggggaa agacccgcac ggatgagcgc gaaaggcact gtctgccgaa cgaggggcga
    16921 cgagtgcgaa aaaaactgat tgcttgtggt tcttaaacct ggtgcgtgaa cccttctgaa
    16981 ggtgtgcgac ccgccgggcc aattctcaaa acaagagtct caaggacagg cgtaaagact
    17041 gtgagtcgca caaggtggca aaccctatca cacgaaaata tgtattgcaa ccgctcggcg
    17101 tcctgcgatg cgccgggccg gccgtgaaaa gccgaaggcc cgcgggggcc gcacacagga
    17161 tttgcgcggc ccccgtgggc cttttagtgt tcctcggccg gggtagttaa gtttctattt
    17221 aaagctcctc ggcctcttcg cgccgccggc gcccgcgacc gcgcagcatc gacagcacga
    17281 tgaggagcat cagcccgacg aacaggatga tgagggcgat cttgatggcc accatcacga
    17341 agccgacgag cgcgatgagc tgcttgagga gcgtgatgac gagcagaatg atggtcagga
    17401 ctccacccca cctggccgca acactgaaga tagctgacat cttttaaatc tccttaacct
    17461 gtgccgactg actcctttga aggccgccgg agtcagaaac ccttgttcgt tacccttaaa
    17521 tcgcccccgg cgattcgcat gatagcagat ggtggcgcgc ccgcgcgaag ccgcgcgcgt
    17581 caatcaagct tcaggcgcgc ggccccgccg gccagatgtt gctctctcgc gccgagacct
    17641 gcgtcacgac ctcgcccgtg ccgaaccgcg tcgcctccgc gccgaacgtc cccgtcccga
    17701 tggccgacgg ccggtgcgtc aggtgctcga agctgtacgg agcctcgcgc ttctgctgcc
    17761 ggttgcgcgt ccagtcgaag agcatgtaag cgccgaggat gacgagcacg acggggagca
    17821 cctgcttcac ggggagctgg atgccgaaga gcgtgtggag caggaagact gtgccgaggg
    17881 cgatgagcac gaagccccag gcgagcgggt tgccgtaaag acgtctggcg atggcgtcgg
    17941 actcggcgtc gggcgcaagg ccggcgcgga tgagctgcgc cgtgcggacg gcgtcgatgg
    18001 cggcgaagag ccacgtgccg accatgccga ggaggaagaa ggcggtgccg ttcgtgaccg
    18061 tcgccatctg gaagaagctg gcgaagatag tgaagtggac gagcgccttc gaggtctggc
    18121 cgttgtaggc cgcgccgagt cccggcgtca gcagcgacaa aagcagcgcg acgaagggcg
    18181 aggtcttgcg acggacgacc tgcgggctgc tcgcgaccga gcgcgcctgc tgctgttgca
    18241 gcagttcgac gcccgcgacg atgcaatcct gacagtgcgg gaagccgcgg atgcggtggt
    18301 cgcacgccgg gcacagcggg cgcgcgcagt ggctgcactg cacgacggcc cggttcgtca
    18361 ggtggtatga gcaattcgtc agcaagactt ggaaacctct gtctaacttt tcgttccacg
    18421 gtcgccggag gcccacgcct accgagaact tcgcgccgct attactacta cttcttcggc
    18481 tcgtcctttg gcgtctcggc tgcgggctgc gacttgttat cgttggccgt cacggcggcc
    18541 gcgccatcgc gcgcgggcgt gccgacaaat gtctcaaggc cctcggcgac gcgcttgagg
    18601 tcagggctgc tggcgcctcg gctgtaggtc tctgcggcga ggcgcacgct ggcgcggtac
    18661 atcccgccga tggagccgtc ctcggagatc gtgttcgtgc cgacgaagac ggcgagcaga
    18721 accatcgttg cgacggtggc gaactgcggc tggacgacca cgtcgagcca cccgcgcagg
    18781 cgctcggcga ggcgagcggc caagggcggg cgcacctcct gcgcctgcgt ggtgccgagc
    18841 gtggcctgaa ggatacgctc gtggagtccc gcggggacgg cctgttcgat gttgatgtag
    18901 gagtagcagg cgccgatgga gcgcacgacc tgaccgggaa gctcggtgca gcgttcgcag
    18961 agcgcggcgt ggcgctccca gcggtggaag agtgtggcgg ggaggaatcc gtcgagatag
    19021 tcggtcaggt gcgtctcgaa atcctcgcac gtcatcgagg tctcgggcgc ggtgcgaagg
    19081 agtatgcgcg cctcgaactc gcgcgaaggg gccggcgctt ccgaggcgcg gcactcgctg
    19141 atcgtgttcc tgacctcgtt cagaatctcg tggcagaggg gacagcgcag cgcgtgctcg
    19201 ccaaaagcac gctgaacctc gacggcgagc gcgccgtcaa ggtaatcggt cagacgatct
    19261 tcaaactctg tgcaaagcat cagtctcgct ccatcccttc accccttcga ttttcctacc
    19321 tgaccctcgc gcggccgtct tcttctgtca aacgtcgctc atcgcgacga cgcgcatgcg
    19381 gcgcaggatt ttcgccagct caattcgccc ccgattgatg cgcgacttca ccgtgccctc
    19441 gggtatcttc aaaagatcga cgatctcctg gtagctcaac tcctcgatgt cgcgcaagat
    19501 cacgcacgtc ctcaagtcgg tgggcagctt gtcgatgccg gcctgcacct gtcggccgag
    19561 ttcctgcctc tcaagctcgc gttgcgggga gccgccggcg gcccggaggt gataggcgtg
    19621 gccttcgagg tcttcggcgt gggtgtcctg cggggcgcgc tggcggcgac ggtagtcgtc
    19681 gatgatgaga ttgcgggcga ggcgcatcag ccagttttgc aaatcgccct gccggggatc
    19741 gtactgttcg agcgagcgat agacgcggac gaagacctcc tgcgtcaaat cctccgccgc
    19801 gtcggcgcgg gaggtgaaac ggtaggcgag gttatagatg cgccgcgaaa aggtctggac
    19861 gatctcctcc cacgcggcgc cgtcgcccgt ccggcatctc ctgaccaact ccgccccgtc
    19921 gctgacagcc actgcgttcg ctgctcccac ctcgcgtgcc ccttcccgtc agtcccgccg
    19981 aaaatgtctt cgggtggttc cggcaaaccc cttgttccaa gccgggatac gacggtcaga
    20041 agggtaaagt tcccccggaa actgtaagcg gtaagcagtg agcggtaagc agtaaaaacc
    20101 cttctccccg ccggcagagt tctttcggaa agcgcgactg actgctcacc gctcactgct
    20161 tagtgcttac tttcaccctg ccttgcgcct gcgcggctaa tattatagcc ttaaactgtt
    20221 tacaccacat ctttgaggtg gccgcggccg aagaagttaa cgagtgcgcc gcttcgggag
    20281 aagagaaggc acacctgcga cggccggagg ccatctgtca tccgctcgca cgccggaaaa
    20341 attgtcgctt gcgtaaatac ttaaagttca tcgccctgag tctcgtggcc gtgctgctgc
    20401 tgtggtggtt cgggcacaac ctcgactggg ccgaagtgtc cgcgtccatc gggcgggccg
    20461 actggcgtct gatcgccggg ggcgtcgtcc tcatctgggt cacgtacctg atacgggcct
    20521 accgctggcg cgccttcctc gggccgctcg cgccgggggc ttcgctgcgt gagattttcg
    20581 ccgcgaccac ggtcggcttc agcgccatct ttctggcggg tcgcgccggc gagttcgtcc
    20641 gccccgccta cttgccgctg cgcgacaaaa atgtgcggcc cggcgcggcc ttcgtcacca
    20701 tcgccgtcga gcgcatctac gacatggtgg ccgtgatcgt gctcttcgcc gtcaaccttc
    20761 tcgtcttccg cgcgcccggc gccgacgccg cctcctacga gcgcgtgcgc gaggcgggat
    20821 tcgtcctgct gctgggcgcg ctcgccggcg tggccgcgct cgtctacctg cggcgtcaca
    20881 cggcggcggt cacgcgttgg attgacgcgc ggctcacgaa agcgcccgcg ctcctgcgcc
    20941 gcgccggaaa aattctgacc ggattaatcg aacaactctc gcacgcgctc ggcgtgctca
    21001 ccgacacgcg cgagctatta gtcaccgccg gctggacggt ggcgctgtgg gcgacgattg
    21061 tcgcggcgaa catgctcgtc atgcgcgcct tcgggctgtc ggtcggcgtg agcggcgtgg
    21121 tcttcgtgct gggctggtcg ctggtcggct cggtggcgcc gacgccgggc ggcggggccg
    21181 ggactttcca cgcggcgacc gcctacggca tgacggcctt cctcaacgtc gacgagacgc
    21241 aggccaaggc ggcgaccatc gtcctgcacc tcgtcgtctt cggctccgcg ctcttcttcg
    21301 gcgcctatta cttcttccgc agtcacgtcg cgctggcccg gctgcgagag atgtcggacg
    21361 aggagcgagc tgagttggag ggagaatcgc acccggccgc cccggcgaca ccgggcgaca
    21421 gggaggcggg cggcggcggt agtgcagcga cggccctgcg cttcgaccgc acttgaaatg
    21481 tccggcgcgg ccggcgtggg tgacgaagag acgcggccgt gtgaatttcg cgcaagggga
    21541 cggttttgcg agcggacgct cttacatctt cgatcctgac ggagttgctc ttatgatgtg
    21601 cccgttctgc gcctatcttg aggacaaggt ggttgactcg cgcgagtcgc gtgagggcga
    21661 cgccatccgg cggcggcgcg agtgtctgcg ctgcgagcgc cggttcacca cctacgagcg
    21721 gatcgacgaa atcccctaca tggtcgtcaa gaaggacggc cggcgcgagc cgttcgaccg
    21781 caacaaggtg atggccggac tcctgcgcgc ctgcgagaag cgccccgtgc cgtcggcgaa
    21841 gctcgacgcc attgtcacct ccatcgagaa gtacgtgcag gagtcgtccg agcgcgagcg
    21901 gccgacctcg aaggtgggcg agatgatcat gcggcgactc aaggaactcg ataaggtggc
    21961 ctacgtgcgc ttcgcctccg tctacctcga attcgaggac gtgtccgagt tcatgcacga
    22021 gctgaagacg ctggtgcgcg cgcgctcaag cgcgggaaag aaatcgtaaa tcgtgaatcg
    22081 taactcgcgg ctgtggctcc gcagacgttc ataagggtca cgggccgtgc atcgacgatt
    22141 gctttttcga ttgacgattg acgattgacg tctcttgaat gaaaagaatc gcgcacacga
    22201 cgggccttga gcggatcgag ctggagcggc tgcgctcgcg cgtggggcgt ctgttcgtga
    22261 tgctggagga ggcggccgag gccgtgtcgc cgcgcgtgcc gggggcttgg ctgccgccgg
    22321 cggacgtgtg cgagtcggaa gacgcggtaa cggtgcgtgt ggagttgccg ggcgtgcgcg
    22381 ccggtcaggt cgaggtcgcg ctgacgaact cgcacctgag aatcagcggc cggaaaagga
    22441 agggcgcacc gcgcgccgcc gccacgcatc tatgctcgga gaggagctac gggcaatttg
    22501 cgcgcgtcat cccgctgcgc tggcccgtgc gcgtcaccga ggcgaccgcc gagctgcgcg
    22561 acggcctgct caccatccgg ctgccgaagc ttctggagcg tcgcggcggg gagttcaagg
    22621 tcgaggtcgc ggccggagac ggcgacgagt agggtttacg aatcattcac agacggcgtt
    22681 accacctgat tggagattga taactgtttt ggcagacaaa gatttcatgg cggcaatcac
    22741 ggacacggcc gatggcgtag tggtggaagc gcaggacgcg ccgggcggcg tggagcagca
    22801 gcttcaggtg cccgacgtgc tgcccgtgct ccccttacgc gacatcgtca tctacccatt
    22861 catgatcgtg ccgctcttcg tctcgcgcga aaaatccatc cgcgccgtgg acgaggcgct
    22921 cggcgagaac cgcatgatcc tcctcgtctc gcagcgcgac ctcgacaagg aggagccggc
    22981 cggcgaagac ctctacatga cgggcaccgt cgccgtcatc atgcggatgt tgaagcttcc
    23041 cgacgggcgc atccgcatcc tcgtgcaggg attggtgcgc gcgcgcgtcg agtcggtgga
    23101 ggacgtggaa tatttaaagg cgcgcgtgag cgtgatgccg gagccgcccg cgccggagcg
    23161 ctcgctcgaa gtcgaggcgc tgatgcgcaa cgtgcgcggc tcggtggaga aggccgccaa
    23221 cctcggcaag aacatctcgc cggaggtgat ggccatcgtc gccaacctcg acgagccggg
    23281 ccgcctggcc gacctcgcgg cgtcgaatct ggagctgaag gtcgaggacg cgcagtcggt
    23341 cttggacatc gccgacacca cggcgcgtct caagcgcgtt aatgagcttc tgaacaagga
    23401 gatcgaagtc ctcaccgtcc agcaggagat caacacgcag gcgcgcgccg acatcgaccg
    23461 ctcgcagcgc gagttctacc tgcgccagca gatgaaggcc atccagtcgg agctgggcga
    23521 gggcaacgag ctggccgagg agatcgcgca gttccgcgag aagatcgagg cgtcgcggat
    23581 gccgaagcac gccgaggagg aagccctgcg ccagctcaag aagttggagc gcatgcaccc
    23641 ggacgcggcc gaaacggcga cgctgcgcaa ctggatggag atcatggttg acctgccgtg
    23701 gtcgaaggct tcgaccgaca accttgacct catcaaggcg cagacgattc tcgacgagga
    23761 tcactacggg ctggagaagg tcaaggaccg catcgtcgag gcgctcgccg tcaggaagct
    23821 caaggagaaa cccaaaggct cgatcctctg tctcgtcggc ccgccgggag tgggcaagac
    23881 gagcctcggc cgctcgattg cgcgcgcgct cgaccgcaag ttcgttcgcc tgtctctggg
    23941 cggcctgcat gacgaggcgg agatacgcgg ccaccggaga acttacgtcg gcgcgatgcc
    24001 cggccgcatc gttcaggcgc ttcaacaggc cgcgacgaac aacccgctca tcatgctcga
    24061 cgagatcgac aaggtcggcg ctgattttcg cggcgacccg tcgtcggcgc ttttggaagt
    24121 cttagacccc gagcagaaca acagtttccg cgacaactat ctcggcgtca cgttcgattt
    24181 gtcgaacgtg atgttcatga cgacggcgaa cgtgctcgac acggtgcagc ccgcgctgcg
    24241 cgaccgcatg gaggtcatcc ggctcgccgg ctacaccgag gaggagaagg tcgagatcgc
    24301 gcgccgccac ctcatcccga agcagattga ggagaacggc atcacggcgc gcaacctcca
    24361 actgtcgaag ccggcgctgg ccggcatcgt caagcagtac acgcaggagg cgggcctgcg
    24421 gcagttcgag cgcgagatcg gcaccatctg ccgcaaggtc gcgcgtcgca tcgccgaggg
    24481 acgcagcgag atcgtgcgcg tcacgcagaa gaacattcac gaatttctgg gcgcgccgaa
    24541 gtttttgcca gaggaggtct tgaagaagga tcagatcggc gtcgccaccg gactcgcttg
    24601 gacggctgcg ggcggcgacg tgctcttctt cgaggcgctc aggatgcgcg gcaagggcgg
    24661 gctggtgctg acggggcaac tcggcgaggt gatgcgcgag tccgcgcagg cggcctactc
    24721 ctacgccaag tcgcgagcgc gcgagctagg catccccgaa gaggacttcg agaagttcga
    24781 catccacctc cacgtccccg agggcgccat cccgaaagac ggcccctcgg ccggaatcac
    24841 cttggcgacc gcgatggtct ccgtgctctc gcaacgaccc atccgtaagg acgtggcgat
    24901 gacgggcgag attaccctgc gcgggaacgt cctgccggtg ggcggcgtca aggagaaggt
    24961 gctggcggcg cgtcgggcgc gcgtctcgaa aatcatcctc ccggcgcaga accgacgcga
    25021 catggacgag ataccaaagg agcttctggg cgagattcag ttcgtcttcg ccgaacacgt
    25081 gcgggaggtc ttcgagcacg cgctcaagga gccggtacgc gcgccggctg cgccccccgt
    25141 cccgcgtagc acgcgccccc cgcaggcggc ccctcagccg agcgcctgag ctagcctgtc
    25201 ggagcctgct tggaatcatc ttcacccgtc gtttagaggc tttgctcctg cccgccggct
    25261 caactctcgc acgtcgagcc ggcgggcagg agcaaagcct ctaaacggga agataaaggc
    25321 ttcattcctg ccgtttagac ggggcgaaat atacctgctg acgggcaaac cgacggcccc
    25381 gccaaatcat ctttatagac tgcaagcgaa gcgattcgag tggaaaaacg ggccgcttcg
    25441 ccttttattt gcaagtagat gaagtttgac accccgtaaa atcgtgtgat aacctccgcg
    25501 atggttccgg ccctcagacg ctccctcgcg cgaggccggt tcattcggga tatttttcct
    25561 cttatacttt cagtggatgc cgctcgtgcg gccctgtcgg gaagggccgc catcgtgggt
    25621 tcgctttagc ctgaaattcg ggagaacacg attgaataat acgagcctcc cccgccttat
    25681 cgccggcgct gccctctcgg cggccttcgt cttcctgccc gtcgcgagcc tccgcgcgca
    25741 ggttgcgcag caatcatatc tccgtcacac gacgagcgac gctgaccgtg aagacccgcg
    25801 cattcaggcg gtcatcgccc gcgccgagag ccacttcaag ctcggagagc tgaacctcaa
    25861 ggacggtaag cgcgagcaag cgcgcgacga gttcgacaag gcggtggacg ccgttttgga
    25921 gtcgggcctc gacgtgcgcg cctacccgcg tcttcagcag tactacctgc aactcgtcga
    25981 gcgcgtctac cgcgtagagg tgccgacgca ggatcagcag cagcgcaccg tcgtcgctga
    26041 aacgcagggc atgccggctt cagacggcgg gcagcaacag gcggcgcagg tcgtccaggt
    26101 cggcttcgtc gatcagaagt tcgagccgtc gcccctcgat gagatgagcc ggctcgtgct
    26161 gaccgagaag gagaaggagg tcacggtcgg acaggtggcc gaagccgagg aagagatcaa
    26221 gaactcgctc gacttccagt tcaagccgca cccactcgtc ctccagttca tcaactacta
    26281 tcagggccgc gggcgcgcga cgatggagac gggcctgcgc cgctcgggcc agttcacgcc
    26341 gatggcgcgc gagattttcc gcaagcacgg cattcccgag gacgtggtct ggttgggtca
    26401 ggttgagagc gcgtggcgcc cgaacgcacg ttcgtgggcc gcggcgtcgg ggttgtggca
    26461 gttcatcccc tccacgggtt cgcgctttgg cctgcgccag accgccttcg tcgatgagcg
    26521 caacagcttc gagaaggcga ccgaggcttc ggccaagtat ctgaagtggc tctacaaccg
    26581 ctatcacaac tgggagctgg cgctcggcgc ttacaacacc ggcgagggca acatcgaccg
    26641 ggcgattgcg cgcgccggca ccaaagattt ctgggccatc tacccgtaca tcgccaaaga
    26701 gacgcgcgac tacgtgccta acattctggc cgtcatcctg atctcgaaga acccgcagaa
    26761 gtacggcttc cgcgacatac agaggatgcc gcgtatcgac cgccagtacg acaccatcag
    26821 cgtgccgtcg gcgacgagtc tgcatctgct ggcgagcctc acggattcga gcagcgacta
    26881 tctgcgctcg atcaacccgg agctgcgccg cgacacgacg ccgcgcggcg agtcgtacca
    26941 cgtgcgcgtg cccgcgggca aaggcaaggt ggcggtcgat ctcctcaagc gcatcccgat
    27001 ggagcgccgc gaacagttgg cccgtgtcgt cacggtatcg ccgggcgaag acttgcaggc
    27061 ggcggcttcg cgcgcgggcg tctcggtctc gcaactccag cagtggaacg gcggagccga
    27121 tttgtcgaag ggcggcaagg tggtcgtccc ggccggcagc gttcagcgga cttcgaagat
    27181 ttacgagcgg ccccgcggca gcgccgtggc cggcagcact atcaagtccg tgacggcgaa
    27241 gggcggcgag acgctcgcgc agctcgccgc gcagcacaac gcctcggtcg aagaggtcgc
    27301 gaagtacaac ggcatagccg ccaacgcgca actcacgcgc gggcagcgca tcagcatccc
    27361 gaccgcggcc ggcgccgcgc ccacagcgcg cgctccgcgc cgccggtaag acaccgcact
    27421 cgtcagacga cggcgacacc tcggtcgagc gcgctttcgg tgaacaactg aaagcgcgct
    27481 caactgtttt agaaccggtt gcgatagggc gcgtgttcgg aacagtaccg cgagcggtag
    27541 cgagtgggtc tgctgacgcg ctaagcgggc ctccgaccac cgccgcaccc gctcgcggta
    27601 ctgtaaaagc acgcgctcgt cacaaatcta aagtaaggga acctcacacc catgctcttc
    27661 cgcacacagt cggcggccgt ctacggcatc gacgccgacc tcgttgacat cgaagtcgat
    27721 ctcgtgccgg cgcgcggcga atcggactcg ccgtcgtcgg taacgattgt cggcctgccc
    27781 gatctggccg tgcgcgagtc gcgcgagaga atccgcgcgg ccgtcaacaa cagcggcttc
    27841 ttcttcccct tccacaagac gaccatcaac ctcgcgccgg ccgacgtgcg caaggagggc
    27901 gcaagcttcg acctccccat cgcgctcggc gtcttaggcg ccaacggcga cctcggcgac
    27961 gccgagggcc tgaccgacat gctctcggtc ggcgagcttt cgctcgacgg gcgcgtccgc
    28021 agcgtgcgcg gggccctaag cgtggcggtg cgcgcgcgcg aggccggcat ccgctgcctg
    28081 ctgctccccg aagagaacgc gaaggaggcc gccgtcgtcg cgggtgtgga cgtgtacgcg
    28141 gtcacagacc tgcgcggcgc cgtcgaactc gtcgccgcgc tgcgcggccc cacgccgccc
    28201 gcgcccctac gcatcgaccc cgccgaggtg ctcgcgcccg cgcgcacctc gggcgtcgat
    28261 ttccgagagg tgcgcggcca actcgccgtc aaacgcgcgc tcgaagtggc gagtgcgggc
    28321 gcgcacaaca tacttctcgt cggcccaccc ggctccggca agacgatgct cgccaagcgt
    28381 ctgccgacga ttttgccgcc gctcgaattc gaggcggcgc tggagctaac gaagattcac
    28441 tcggtggcgg gcctcaccgg ccgctccggc ctcgtcacgg agcgcccttt tcgctcgccg
    28501 caccacacca tcagtgacgc cggcctcatc ggcggcggcg ccatcccgcg ccccggcgag
    28561 gtctcgctcg cgcaccatgg cgtcctcttc ctcgacgagt tgcccgagtt cgaccgctcg
    28621 gtcctcgaag tcctgcgcca gccgctcgaa gaccagcgcg tgacgatcag ccgcgccgcg
    28681 atgaccttga ccttcccggc ctccttcatg ctcgcggcgg cgatgaatcc gtgtccctgt
    28741 ggattctgga acgaccccac gcgcgagtgt cgctgcacgc ccttgcagat tcaacgctac
    28801 gtcggccgca tctcagggcc gctcctcgac cgcatcgaca tccacatcga cgtgccggcc
    28861 gtgaagttta aagagctgtc gggcgcctcg acgcacgaag gtgaaagctc ggaggagata
    28921 cgagagcgcg tgattcatgc acgcgagcgt caacgcgagc gattgcaggg tgagggaatc
    28981 ttctcgaacg cgcagatggc gacgcgcctg attcgcaagt attgccgaat tgatgcggat
    29041 agcgagcgga tgttggagca ggcgatgact cgtctgggcc tgtcggcgcg agcttacgac
    29101 cgtatcctca aagtcagccg caccatcgcc gacctcgacg gcgctgctga catccgcccc
    29161 gcatacgtcg ccgaggccgt cggctaccgc tcgctcgacc gcacttactg ggcgtgagag
    29221 gcgtcaggtt tggagcgaat tccgatggcg tcggtgtttg aacgtcgccg cgcggatggg
    29281 gaacacggat aagacggatg aaacggatcg acacggattg aatccgtctt catccgtccc
    29341 atctgtttca tccgtgttcc ctcccgaccc ttgcacgccc ggccttaaca accgcccccg
    29401 aatttgatac tctacgcccc acttcgataa cggcggcgca ctattactaa tgccccaact
    29461 ggccctcacc agagtcatac gcatcgcggt cacgcggctg aaggcttcca gtcagctttg
    29521 atcgggacac gggtgaattg tatcgcctgc ccgaacgccg acgccgttgt gaccgcaaac
    29581 cgagacgagt atgcagcggc gactttgttc gtcgcagtac gagaacgcta aggcaatcct
    29641 cactcggccg aaaccgcaag gggccttccg aattattggg acggccatcc acaccttgcc
    29701 ggaaccaccg ggctgcttta ttgccttacc tcaaccagaa actaatggag gcgaagaatg
    29761 cggaagggat tgctgacagg tttaataggc gtagccttgg tggccgcctc gatgtttccc
    29821 tcgatgaact cggacgcggc gcgcgctcct caggggggag agatttccaa caaccggtcc
    29881 cagcgcgcgg ggctacccaa ccacgacatc cgtctcgccg gccggggcga gttcactgac
    29941 gttgacgtca gcccggtcgc cggggcgcgg cgcgccgtgc cgaacgccgg cgcggcggtt
    30001 caggcgcgcg ccgcggcggt cgaaaatttc cgcgccggtc tccggccgga acaagcgagc
    30061 aacctgcgcg ccgaggtcaa cgaagcgggc gcgctgaaga atatcttcat cgacggcgca
    30121 tcgctctccg ggccgcagtc ggacgcccct gaccagatcg cccgaggatt cctgaagcgg
    30181 gaggccgcgc tgttcggtct ctcccacgcg gcggtcgatg aactcaaact cgaaaaggaa
    30241 gacaacgacg cgggaaccac cttcctcgat tacgcgcaga cggtcggcgg cctgaaagtg
    30301 tttgaaggga acgtgaaggt ggccgtcaac agaaacggcg aagtcctctc cgtccgcgaa
    30361 ggtttcatcg tcgccgggca gaggggcagg ctccggccga cgctcacgga aacggagggc
    30421 atcgccaaag ccttcgagca cgccggccgg gcggtgctcc cctcggcgct cgccgagacc
    30481 cgcggtcgcg cctcccagag cgacaacgcc gcgttcgcca accctttggg ggcgggcttc
    30541 gaggaagtgc tttccgagct gaacgtcgtg cgtgtgggcg acgcggcccg gctcgcgtgg
    30601 cacgtctacg ccgaagtcgg ccccgaagag tggtacgaac tcctcgttga cgcgcacagc
    30661 ggcgagttgc tcctgcgtca caacctctac gtgcatcagg cgcagggcac cgtctacacc
    30721 gaggcgccgg acaagggcgc gcgcgtgctc gtctccttcg tcggcgacac cgtcatcaac
    30781 acgagcgccg gctggatggg cacgtccacc gtgacgacgg gcaacaacgt cgaagcctac
    30841 ctcgaccgcg acgccaacaa ctcgcccgat accactagcg gcaacggcct ctcgaacggc
    30901 cacgcctccg ccgccaacca gaacttcacc ttccccttcg acacggccgt ctcgccgacc
    30961 accgccacga atcaggcggc cgtggtgacg aacctcttct actacaacaa catcatgcac
    31021 gacttctcct acaacctcgg gttcacggag acgtcgcgca acttccagac caataactac
    31081 gggcgcggcg gcctcggcgc cgactccgtg agggccgagg cgcaggacgg ctccggcacc
    31141 aacaacgcca acttcgccac cccgcccgac ggccagcggc cgcgcatgca gcagtacatc
    31201 ttcaccacgc ccaaccccga ccgcgacagc tcgatggacg gcgacgtggt cttccacgag
    31261 tacggccacg gcatctcgaa ccgtctcatc ggcaacggca gcactgctct gcaaggcacg
    31321 cagtcgggcg cgatgggcga gggctggtcg gactactggg cgatcacgat caacggcgac
    31381 ggcgcggtgg gcgagtattc caccggcaac ccgaacggca ttcgccgcgc gccctacacc
    31441 gtcccggcca acaccgttca cgactcttac gccgatgtgg gcgtgggcgg tttccaggtt
    31501 caccgcgacg gcgaggtctg ggccgcgacg ctctgggacc tgcgcacgca gctcggcgcg
    31561 accatcacgg acaggatcgt cctcaacggc atgaagttca cgccgacccg cccgtcgttc
    31621 ctcaacgcgc gcgacggcat cctgcaagcc gaccagaact taaacggcgg ggccaaccgc
    31681 tgcgccatct ggacggtctt cgcgcgtcac ggcatgggcg tctcggccgt cggcaacgac
    31741 ggcacgacgc acaccgccgc ctctgatctt ccgtcggact gcggcggcac gtgcagctac
    31801 tcgattagcc cgaccagcgc cagcgtcgcg gccgggagtg gcacgggcac cgtcaccgtc
    31861 acggccggcg tgggctgcgc ctggacggcc gtgagcaacg ctacgttcat cacgatcacc
    31921 ggcggcgcga gcggtagcgg caacgggacg gtgtcctact cggtcgctgc caacaccgcg
    31981 acgacatcgc gcgcgggcac gctgaccatc gccggcctca ccttcacggt gaatcaggcg
    32041 ggcacgggtg gcggcggcac caacgtcatc gtcaaccccg gcttcgagtc gggcacgacg
    32101 ccgtgggtca tctccagtca ggtcacgcgc agcaccgggg cctacccgca cagcggcgtg
    32161 gcctacatga tcatcaacgg ggtcaacagc acgagcggca cgctctacca gacggtgacg
    32221 attccggcgg gcggcgccaa cctcaacttc tggctgaaca tcaccaccag cgaggcggcc
    32281 ggcgcggcca tctacgaccg gctcttcatc gaagtgcgta acacgtcggg cacactgctc
    32341 gggacgctcg cgaccttcag caaccagaac agcggcacgg cgggagtgta cgtactgcgc
    32401 gggccgtaca acctcaacag ttgggccggg cagacggtgc gaattcagtt ccgcggcacc
    32461 aacgacatca cgctgcccac gtcgttccgc atcgacgacg tgtcgctcca gtagacgcag
    32521 acgagagcgc ccggcctccg tcgagaaggc cgggcgcttt caaaacttaa atacttaacc
//
LOCUS       DASVES010000033         5331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_158008, whole genome shotgun sequence.
ACCESSION   DASVES010000033 DASVES010000000
VERSION     DASVES010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158008"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1744
                     /locus_tag="VF240_01495"
     CDS             173..1744
                     /locus_tag="VF240_01495"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX7173944.1"
                     /translation="MPADAVETLKAPGEPPFVLALDVGSSSVRAAFFDSQGDEVEGTR
                     ARVERGFRTTADGGSELDAECALAEAASVIDDALALAPERVLAGTLAVAVACFWHSLV
                     GVAADGRAVTPVYGWADTRAARQAEALRRELDERETHARTGCRLHPSYWPAKLRWICE
                     ERPHEWRAAARWMSFGELLTVRLCGGDVNGDGNVPAAVSMASGTGLLDQHNCAWDAAL
                     VERLGLTVEQMPSLARDSQTLTLANDYAARWPSLRGVPFFPAIADGAANSVGEGCATR
                     ARIALMVGTSAAMRVVYEGEPPAELHESLWCYRLDRRRVIVGGALSDGGALFDWLRDS
                     LKFSLPNDVAAMDVATFGAKELEEEVARLEPDAHGLTILPFWSGERSTGWHARARGAV
                     LGLGAHTRPAEIVRAAQESVAYRLALIFDALIAHAPGASLRASGGALLRSPAWARIIA
                     DVLGRPVTLSPVEEASSRGAVLLALEALGKIKDVADVDVPGGETIEHEAQRHAVYRRA
                     RERQQKFYDLLIREL"
     gene            1780..3825
                     /gene="tkt"
                     /locus_tag="VF240_01500"
     CDS             1780..3825
                     /gene="tkt"
                     /locus_tag="VF240_01500"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7173945.1"
                     /translation="MSASTTSRETTPTPELDQLCVNTIRTLSLDAVQRAESGHPGLPL
                     GAAPMAYVLWTRHLRHNPRNPAWAGRDRFLLSAGHGSMLLYSLLHLTGYDLSLDDLMN
                     FRQWESKTPGHPENFQTPGVEITTGPLGQGFANGVGMAMGAAHLADKFNQEGFPLIDN
                     FVYAIVSDGDLMEGVASEAASLAGHLKLGRLIYLYDDNRVTIEGFTSLAFTEDVARRF
                     DSYGWHTLTVEDGNDLEAIDAAIREAQGVTDRPSLISVKTVIGFGMPTAGTRKAHSDA
                     PGEEAVKETKRQLGWPEDETFHVPEEARAHFGGSVERGERLEAEWNALVERYEREHEE
                     LGRSWRVTMSGELPEGWESHLPSFEAAKPMATRAASGECINALAPHLPMLFGGSADLG
                     VSNNTDIKDGGSFQSDNYAGRIIHFGVREHAMGSTMTGIALNGGLIPFGGTFLCFSDY
                     MRPAVRLAALSGVQAVYVFTHDSVGLGEDGPTHQPIEHVASLRAIPNLHVIRPADVHE
                     TREAWRAAILRREGPTALILTRQKVPLIDRAKFAPADGARRGAYTLADATDADGKPTA
                     PRLVIIATGSEVALALDARERLQAEAAPTRVVSMPCWELFEEQTAEYRESVIPSSVAA
                     RLAVEAGVPLGWERYVGPGGGIVGLDRFGASAPGDVALKNLGFNVGNVVARARALIK"
     gene            3934..4227
                     /locus_tag="VF240_01505"
     CDS             3934..4227
                     /locus_tag="VF240_01505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX7173946.1"
                     /translation="MTEQSLRVVARIVAKPGRVEEVRELLRALVGPTRRERGCVSYEL
                     LQNRDDPTDFTFVEEWTDAAALDAHLNSAHIQSLPPEFDEMADVDIRKYSLIA"
     gene            complement(4249..5295)
                     /locus_tag="VF240_01510"
     CDS             complement(4249..5295)
                     /locus_tag="VF240_01510"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7173947.1"
                     /translation="MSEGASLLVVVNHAAARARRAWPGVRAALDAAGVPFDAHETRAP
                     GDAHARTREALRAGYRTIAAVGGDGTLSEVASGFFEDCEALPRGALPAKTSAPRAALA
                     LLPAGTGDDFARGLSGGRRVPLESWTARLVAHCRTARAGEAGMTRRSDADITRGSDAD
                     TTRSVDVLLGETDAGARRFVCLNAATLGIGAEVAARVGAQGERLRRLPGEARFALAAL
                     AALVAWRDRSVRLRVDDGEWRECRTNIMAVTNGTHAGGGMNLSPDARVDDGRLDVLTA
                     CNLTPTQILRELARVHTGGHVNNPQVSCERGVRVQIETIDGGAGLGVEADGDVRGRTP
                     AEFRVMPAALRVVW"
BASE COUNT          821 a         1969 c         1737 g          804 t
ORIGIN      
        1 agttcacgca gagggttgaa tgaactcaac cctctgcgtg aactccgcgc cctctgcgcc
       61 tctgcggtga atcaatgtcg ctttcactca agtgaacctc gctctaaggg cgcgcgcgac
      121 ttgacgccct tttcgctgaa ctcacgaacc gcatttccgt tatcatgcgt gcgtgcctgc
      181 cgacgcggtt gaaacactga aagctccggg cgagccgccc ttcgtgctcg cgctcgatgt
      241 cggctcgtcg agcgtccgcg ccgccttctt cgactcacag ggcgacgagg tcgagggcac
      301 gcgcgcacgc gtcgagcgcg gctttcgcac gaccgctgac ggcggctccg aactcgacgc
      361 cgagtgcgcg ctcgccgagg ccgcttccgt catcgacgac gcgctcgcgc tcgcgccgga
      421 gcgcgtgctc gcaggcacgt tggccgtggc ggtcgcctgc ttctggcaca gcctcgtcgg
      481 agtagccgcg gacggccgcg ccgtcacacc cgtctacggc tgggccgaca cgcgcgccgc
      541 gcgacaggcc gaagccctgc ggcgcgaact cgacgagcgc gagacgcacg cgcgcacggg
      601 ttgtcgccta cacccttcct actggcccgc caagctccgc tggatttgcg aggagcggcc
      661 ccacgagtgg cgcgcggcgg cgcgttggat gtcgttcggc gaactactca ccgtgcggct
      721 gtgcggcggc gacgttaatg gcgacggcaa cgttccggcc gccgtctcga tggcctcggg
      781 gacgggcctc ctcgaccaac acaactgcgc gtgggacgcc gcgctcgtcg aacgtctcgg
      841 actgaccgtg gagcagatgc cctcgctcgc gcgcgactcg cagacgctca cgctcgccaa
      901 tgattatgcc gcgcgctggc cgagccttcg cggcgtgccc ttcttcccgg caatcgccga
      961 cggtgcggcc aacagcgtcg gcgagggctg cgcgacgcgc gcgcgcatcg cgctgatggt
     1021 cggcacctcc gccgcgatgc gcgtcgtcta cgagggcgag ccgcccgcgg agttgcacga
     1081 gtcgctgtgg tgttatcggc tcgaccgacg gcgcgtcatc gtcggcggcg cgctctcgga
     1141 cggcggcgcc ctgttcgact ggctgcgcga ctcgctcaag tttagtctgc ccaatgacgt
     1201 tgccgcgatg gacgtagcaa cattcggcgc gaaggaactc gaagaagagg tcgcgcgtct
     1261 tgagcctgac gcgcacgggc tgacaattct gcccttctgg tcgggcgaaa gaagcacggg
     1321 ctggcacgcg cgcgcgcgcg gggccgtcct cggcctcggc gcgcacacgc gccccgccga
     1381 gatcgtccgc gccgcgcagg agtcggtcgc ctaccgcctc gcgctcatct ttgacgcgct
     1441 catcgcgcac gcgcccggcg cgagcttgcg cgcctcgggc ggcgcgctcc tgcgctcgcc
     1501 agcgtgggcg cgaatcatcg ccgacgtgct gggccgcccc gtcacgctct cgccggtcga
     1561 ggaggcgtcg agccgcggcg cggttttgct tgcgctcgaa gctctcggca agataaaaga
     1621 tgtggccgac gttgacgtgc ccggcggaga gacgattgag cacgaagcgc agcgtcacgc
     1681 cgtctaccgt cgcgcgcgcg agcgtcagca aaaattttac gaccttctca tccgtgaact
     1741 ctaaacgaag cctgcccgaa acgaggacaa ccgaagatca tgagcgcgtc caccacgtca
     1801 cgcgagacca cacccacgcc cgagttagat caactctgcg tcaacaccat ccgcacgctc
     1861 tcgctcgacg ccgtgcagcg cgccgagtcg ggtcatcccg gtctgccgct cggcgccgcc
     1921 ccgatggcct acgtcctctg gacgcgtcac ctccgtcaca acccgcgcaa ccccgcctgg
     1981 gccggccgcg accgcttcct gctctccgcc ggccacggct cgatgctcct ctactcgctc
     2041 ctccacctga ccggctacga cctctcgctc gacgacctga tgaactttcg gcagtgggag
     2101 tcgaagacgc cgggccaccc cgagaacttc cagacgcccg gtgtggagat caccacgggg
     2161 ccgctcgggc aggggttcgc caacggcgtc ggcatggcga tgggcgccgc ccacctcgcc
     2221 gacaagttca accaagaggg ctttcccctc atcgacaatt tcgtctacgc catcgtctcc
     2281 gacggcgacc tgatggaggg cgtcgccagc gaggccgcct ccctcgccgg ccacctcaag
     2341 ctcggccgtc tcatctatct ctacgacgac aaccgcgtga ccatcgaagg cttcacctcg
     2401 ctcgccttca ccgaggatgt ggcccgtcgc ttcgactcct acggctggca cacactcacg
     2461 gtcgaagacg gcaacgacct cgaagccatc gacgccgcca tccgcgaggc gcaaggcgtc
     2521 accgaccgcc cgtcgctcat ctccgtcaag accgtcatcg gcttcggcat gcccaccgcc
     2581 ggcacgcgca aggcccactc ggacgcgccc ggcgaagaag cggttaaaga aaccaagcgg
     2641 caactcggct ggcccgagga cgaaactttc cacgtgcctg aggaagcgcg cgcgcacttc
     2701 ggcggctcgg tggagcgcgg cgagcgtctt gaggccgagt ggaacgcgct cgtcgagcga
     2761 tacgagcgcg agcacgaaga gttgggccgc tcgtggcgcg tgacgatgag cggcgagctg
     2821 cccgagggct gggagtcgca cctgccttcg ttcgaggccg ctaagccgat ggcgacgcgc
     2881 gccgcctcgg gcgagtgtat caacgcgctc gcgccgcacc tgccgatgct cttcggcggc
     2941 tcggccgacc tcggcgtctc taacaacacc gacatcaaag acgggggcag tttccagtcc
     3001 gacaattacg cgggccgcat catccacttc ggcgtccgcg agcacgcgat gggttccacc
     3061 atgaccggca tcgccttaaa cggcggactc atccccttcg gcggcacctt cctgtgcttc
     3121 tcagactaca tgcgccccgc cgtccgcctc gcggccctct ccggcgtgca ggccgtctac
     3181 gtcttcacac acgactccgt gggcttgggc gaagacgggc cgacgcacca gcccatcgaa
     3241 cacgtcgcct ccctgcgcgc catcccgaac ctgcacgtca tccgcccggc ggacgtgcac
     3301 gaaacgcgcg aggcgtggcg cgcggccatc ctccggcgcg aggggccgac cgcgctcatc
     3361 ctcacgcgcc agaaggtgcc gctcatcgac cgcgcgaagt tcgccccggc cgacggcgcg
     3421 cgccgcggcg catacacgct cgccgacgcg accgacgctg acggcaaacc gaccgcaccg
     3481 cgactcgtca tcatcgccac cggctccgaa gtcgcgctcg cgctcgacgc gcgtgagcgt
     3541 cttcaggcgg aagccgcgcc cacacgcgtc gtctccatgc cctgctggga gttgttcgag
     3601 gagcagacgg ccgagtaccg tgagagcgtc atcccatcga gtgtcgcggc gcggctcgcg
     3661 gtcgaggccg gcgtcccgct gggctgggaa aggtacgtcg gccccggcgg tggtatcgtc
     3721 ggccttgacc gcttcggcgc ctccgcgccc ggcgacgtgg cgctcaagaa cctcggcttc
     3781 aacgtcggga acgtcgtcgc gcgcgcgcgc gccctgatca agtagaacga ccgcgcgtcc
     3841 gtaacagtac cgcgagcggt agcgagcggg tctactctcg tcacccgacc cgctcgctac
     3901 cgctcgcggt actgactgat gcaggaggcg ctgatgacag agcaatcgct gagggtcgtc
     3961 gccagaatcg tcgctaaacc cggtcgagtg gaagaggtgc gcgaactgtt gcgcgcgctt
     4021 gtcggcccga cccgccgcga gcgcggctgt gtgagctacg agctgctcca aaaccgggac
     4081 gacccgaccg acttcacctt cgtcgaagag tggacggacg ccgccgcgct cgacgcgcat
     4141 ctcaactccg cacacatcca gtcgctccct cccgagttcg acgagatggc cgacgtggac
     4201 atccgcaaat actcgctcat cgcctgacgc gccacacgcc cgacgcgctc accagacgac
     4261 acgcagggcc gccggcatca cgcgaaactc ggcgggcgtg cggccacgca cgtcgccgtc
     4321 ggcctcgacg ccgagacccg cccccccgtc aatcgtctca atctgaactc gtactccccg
     4381 ctcacaactg acctgcgggt tgttgacgtg gccgcccgtg tggacgcgcg cgagctcgcg
     4441 caggatttgc gtgggcgtaa gattgcaggc ggtgagcacg tcgaggcggc cgtcatcgac
     4501 gcgcgcgtcg ggagagaggt tcatgccgcc gcccgcgtgc gtgccgttgg tcacggccat
     4561 gatattggtg cggcactcgc gccattcgcc gtcgtccacg cgcaggcgca cgctgcggtc
     4621 gcgccacgcg acgagggcgg cgagcgcggc gagggcgaag cgcgcctcgc ccggcaggcg
     4681 gcgcaggcgc tcgccttgcg cgccgacgcg cgcggccacc tcggccccga tgccgagcgt
     4741 cgcggcgttg agacagacga agcggcgcgc gcccgcgtcc gtttcgccga gcagcacatc
     4801 cacgctccgc gtcgtgtctg cgtcgctccc gcgcgtgatg tcggcgtcgc tccggcgcgt
     4861 catgccggcc tcgcctgcgc gcgctgtccg gcagtgggca acgagacgcg cagtccaact
     4921 ctccagcggc acgcgccggc ccccggagag gccacgcgcg aagtcgtcgc ccgtgccggc
     4981 ggggagcagg gcgagcgccg cacggggcgc gctcgtcttc gcggggagcg cgccccgtgg
     5041 cagcgcctca cagtcctcga aaaagccgga ggccacttcg ctgagcgtgc cgtcgccgcc
     5101 gacggccgcg attgtgcggt agcccgcgcg cagggcctcg cgcgtccgcg catgcgcgtc
     5161 gcccggtgcg cgcgtctcgt gtgcgtcgaa cgggacgccg gccgcgtcga gcgccgcgcg
     5221 cacgcccggc cacgcccgac gcgctcgcgc ggcggcatgg ttgacgacga cgagcaggct
     5281 tgctccttcg ctcatgcgtc aatcgtaaat cgtgaatcgt aaatcgtaaa t
//
LOCUS       DASVES010000034         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_549235, whole genome shotgun sequence.
ACCESSION   DASVES010000034 DASVES010000000
VERSION     DASVES010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549235"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /gene="glgX"
                     /locus_tag="VF240_01515"
     CDS             <1..1707
                     /gene="glgX"
                     /locus_tag="VF240_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX7173948.1"
                     /translation="AGRHGEQVAEFKTMVRALHREGIEVILDVVYNHTIEGNHLGPTL
                     SFRGIDNASYYRLDARDPARYVDYSGCGNALNVRHPRTLQLIMDSLRYWIQEMHVDGF
                     RFDLAAALARELHEVDRLSAFFDIIHQDPVISQVKLIAEPWDLGEGGYQVGNFPVLWS
                     EWNGIYRDAVRSFWRGDAGRIDDLAFRLTGSSDLYERSGRRPYASVNFVTAHDGFTLA
                     DLTSYNEKHNEANGEGNRDGHDHNLGWNCGVEGRTDDPDTLELRARQRRNFLATLLLS
                     QGVPMLLAGDEMGRTQHGNNNAYCQDNELSWVDWNLDESQRELLEFTRQLIRLFHTQP
                     VLRRRKFFHGRQVRGAEIKDVSWLRPDGEEMTAEDWRDDQTRSLCVRLAGDAIDETDE
                     RGRAVFGDTLLILLNVHHRPVSFHLPAHREDVHWEQLFDTREPAGRRKTRRLVKGGAS
                     YRTEAHSLALFRLQVEVEATPVEAGDVRSAREAMEPTGTATTTGPLTQSVYCARAERI
                     VPLDEGFCRACSRQVAEGDGIHHEPTPEVLKRMGYVTTALPEPEDRGEETPGDESQSE
                     EG"
     gene            complement(1714..>2131)
                     /locus_tag="VF240_01520"
     CDS             complement(1714..>2131)
                     /locus_tag="VF240_01520"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7173949.1"
                     /translation="FGGDPRDTHLPHNYTHNTVVYTGTHDNDTTLGWLRARSEDAADG
                     DEGLRRERANCLKYLDTDGSEIHWDFIRAAHASVADISIVPLQDVLGLGSDARMNIPA
                     STEGNWAWRFREGALTEALRDRLREMTETYGRLPSQ"
BASE COUNT          369 a          755 c          677 g          330 t
ORIGIN      
        1 gccggccgac acggcgagca ggtcgccgag tttaagacga tggtccgcgc gctccaccgc
       61 gaaggcatcg aggttatcct cgacgtggtc tacaaccaca ccatcgaggg caaccacctc
      121 ggccccacgc tctccttccg cggcatcgac aacgcctcct actatcgtct cgacgcgcgc
      181 gaccccgcgc gctacgtcga ttactccggt tgcggcaacg ccctcaacgt gcgccacccg
      241 cgcacgctcc agctcatcat ggactccttg cgttactgga ttcaggagat gcacgtcgat
      301 ggcttccgct tcgacctcgc cgccgcgctc gcccgcgaac tccacgaggt tgaccgcctc
      361 tccgccttct tcgacatcat ccatcaagac cccgtcatct cgcaggtcaa gctcatcgcc
      421 gagccgtggg acttgggcga gggcggctat caggtcggca acttccccgt cctgtggtcg
      481 gagtggaacg gcatctaccg cgacgcggtg cgctccttct ggcgcggcga cgccggccgc
      541 atcgacgacc tcgccttccg cctcacgggc agcagcgacc tctacgagcg cagcggccgg
      601 cgtccttacg ccagcgtcaa cttcgtcacc gcccacgacg gcttcaccct cgccgacctc
      661 accagctaca acgagaagca caacgaagcc aacggcgaag gcaaccgcga cggacacgac
      721 cacaacctcg gttggaactg cggcgtcgag ggccgcacgg acgaccccga cacgctggag
      781 ctgcgcgcgc gccagcgccg aaacttcctc gccacgctgc tgctctcgca gggcgtgccg
      841 atgcttttag ccggcgacga gatgggccgc acacagcacg gcaataacaa cgcctactgt
      901 caggacaacg aactgtcgtg ggtcgattgg aaccttgacg aaagccagcg cgagcttctg
      961 gagttcacgc gccagctcat ccgacttttc cacacgcagc ccgtcctgcg ccgccgcaag
     1021 ttcttccatg gccggcaggt tcgcggggcc gagatcaaag acgtgtcgtg gctgcggccg
     1081 gacggcgagg agatgacggc cgaggactgg cgcgacgatc aaacccgctc gctctgcgtc
     1141 cgcctcgccg gcgacgccat cgacgagacg gacgagcgcg ggcgcgccgt cttcggcgac
     1201 acgctcctca tactgctcaa cgtccaccac cgccccgtct ccttccacct ccccgcgcac
     1261 cgcgaagatg tccactggga gcagctcttc gacacgcgcg agccggccgg tcgccgcaag
     1321 acccgacgcc tcgtcaaagg cggcgcctcc taccgcaccg aggcgcactc gctcgccctc
     1381 ttccgccttc aggtggaggt cgaagccacg ccggtcgagg ctggcgatgt gcgctcagcg
     1441 cgtgaggcga tggagccgac agggacggcg acgacgacgg ggccgctcac acagtcagtc
     1501 tactgcgcgc gggccgagag aatcgtcccg ctcgacgagg gcttctgccg cgcctgtagc
     1561 cggcaggtcg ccgaaggcga cggcatccac cacgagccga cgcccgaagt gctcaagcgc
     1621 atgggctacg tcacgaccgc gctccccgag ccggaagacc gaggggaaga aactcccggc
     1681 gatgaatcac aaagtgaaga gggttagcga tgactactgt gaaggcaggc gtccgtaggt
     1741 ctcggtcatc tcccggagtc ggtcgcgcag cgcttcggtg agcgcgcctt cgcggaagcg
     1801 ccaggcccaa ttgccttcgg tgctggcggg gatgttcatg cgcgcgtcgg agccgaggcc
     1861 gagcacgtcc tgcaagggca cgatgctgat gtcggcgacg gaggcgtggg cggcgcggat
     1921 gaagtcccaa tggatttcgg agccgtccgt gtcgagatac ttgaggcagt tggcgcgctc
     1981 gcgccgcaag ccttcgtcgc cgtccgcggc gtcctcagag cgggcgcgca gccagccaag
     2041 cgtcgtgtcg ttgtcgtgcg tgccggtgta gacgacggtg ttgtgcgtgt agttgtgcgg
     2101 caggtgcgtg tcgcgcgggt caccgccgaa g
//
LOCUS       DASVES010000035         7548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_549119, whole genome shotgun sequence.
ACCESSION   DASVES010000035 DASVES010000000
VERSION     DASVES010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549119"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4807)
                     /locus_tag="VF240_01525"
     CDS             complement(<1..4807)
                     /locus_tag="VF240_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX7173950.1"
                     /translation="MFAVALLASPFLFLERGAHVSESARAVSRRETLSRGAEGRGAAA
                     RPDALAQVDPATRARLTEAYGQLPMRFEANRGQTDAAVKFVARGQGYALFLTPTEAVL
                     SLRQAGGREESAADAGGPEGASQAGAAEESPSPSAASSPSGAVLRVGLVGANPAPALE
                     GVEELSGRSNYFVGADERGWVTDVAGFGAVKYESVYPGVDLVYYGQQRQLEYDFRLDA
                     GADPARIALHFEGAQTVALGAGGDLVLGTEVGEVRQRRPFIYQEIDGARREVAGGYKL
                     LGENRVAFEVGEYDQSRPLVIDPVLVYSTYLGGNDSDAGFGVAVDAAGNAYVAGDTFS
                     TDFPGPSPIQAAKDVSFDAFVLKLNPTGSGLVYATYLGGNGDDFGNAVAVDPAGNAYV
                     VGYTTSDIFPLTAGALQQSRLGVIDGYVAKLNPTGSALVYSTLLGGNNNDEVLAVAAD
                     AAGNAYVAGYTDSSEIASAGVFDMRAGSPIYRSADGGGSWAEADNGFTASRVNFFAAH
                     PTDAATIYAGAASGVFKTTDGGNLWSLTGAAQPSTLPRFVNWVAVHPTTPETVYAATF
                     AGAYKSTDGGQTYEEKNTGLFDQNLLTIVIDPATPTTLYAGSFFGANKSTNGGDNWTQ
                     INNGLRAASFSFEPQVFDLVIDPTNPSVLYAGTSQGIFKTTNGGANWAPLNTGLGSAF
                     TPPSVTTLLIDPQNPATLYAAVTVFGGNQLLKTTNGGGEWNVVGAGLRTTIGATTFTP
                     RIDALAIDPANSSVLYAGTLGSGVFKSTDGGANWAQASAGLNNNSVGAIVVDRAVPSH
                     VFAGTNIGSDAFAGKINPEGTAVVHFRFLGGNENDMATGVVADPAGNAYITGHTASNN
                     FPVLGALQPAKGGGTDAFLTKLNPSGAGLVYSTYLGGFANDEARAVALGPAGSAYLTG
                     RTQSTNFPLASPLKFSPPSFIDDAFVSRISADGTTLLYSTYLGGDSGTEQGFGIAVDA
                     AGNAHVTGLTSSNDFPLVGAVQTTRGGGNDAFVSKLNPSGTALAFSTYHGGAGNDVGN
                     AVAVDAAGNTYFVGATNSFNLPLAAPFQNAYRGGGDAFIAKLGSSIELAVAMTDSPDP
                     VAFGSNLTYILTVSNNGELSAAGVTLTDTLPAGATFVSATPGQGSCSGTTTVTCNLGT
                     LNGGAATTVAIVVLPPAVRNISNTASVTSNEADPLPANNTATESTQVNFADLSVTGAA
                     SYGKVAPGARINYLFRVANNSGGPAESATLSGNLPAGTTFVSCGAVGGVCSNSGGSWA
                     VTFPSLAVGQSAAVVLVAAVDAAVAEGAVLGHTATAAAAATPDPDAGNNSAAVTVTVT
                     ATPLRVKTNGLIAFASDGVHTVKADASAPPAPFPGAEGGIFPRWSPDGTRLAYTVQKR
                     QPTYPNDAYYVIEAANADGSGRLTLADNVIDTSFDNRSGFSWSPDGTRLAYVGTDRLI
                     YLANADGSGYAKLPGSPTQVRDVDWSPDGSRFVFSKDFGEIYVMNVDGSGLSKLVAFG
                     TGPDGPTHYLSPLWSPDMTKILFVQRSNNFTDILFMRSDGTGAQRLLNRGQTSGPAWS
                     PDGKRIAFYHGTEMHVVDFDGTDELTLVTNQSCCGFGGTSWQPIPTDAPL"
     gene            5220..6164
                     /locus_tag="VF240_01530"
     CDS             5220..6164
                     /locus_tag="VF240_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7173951.1"
                     /translation="MLTGGAAGVATLAAVNAAVAREAPEPETGALGGEAGAYEWRHGG
                     VFFRHAGPDAAPPVVFIHGVGAGARSFMWRRNFEPLSQDFRVYAPDLLGFGYSDKPSN
                     VPYSADLYVEMIVDFLRDVVGRRAHLVAGSLSAAFAVRVADEYPGLVDSLVLISPTGV
                     GQMDSRPDLPGAAFYGLLHSPVLGTSFYNAMTSERGIRDYARRQLFYDPRFATPRLVA
                     HYYAVSHLPGAHYAATAFLSGYLNSDIREPFARLRQPATLVWGRQDEANPATRSRALL
                     HSNPRARLEIFDRCRQMPQEEHAERFNALLREAFSAAR"
     gene            6294..>7548
                     /locus_tag="VF240_01535"
     CDS             6294..>7548
                     /locus_tag="VF240_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7173952.1"
                     /translation="MTPKTKLFLLLVTLWFAASAPEATAQAARTTARAQVRGGASAPA
                     LKPHRIKLASGRSFDLNLPEGFQISVAAQGLKRVRFMAQSPDGRVFVTDMHNLADNER
                     GIVYVLDGFDPARNAFARVTKYLTGLRNPNSVAFHTDRDGASWLYLALTDRLLRYRYR
                     NGDNAPTGAPQVLARFPDYGLNYKYGGWHLTRTIAVGANGRIYVSVGSSCNACEEKEE
                     VRASIIEMDADGGNQRQYARGLRNAVGLRFAGGRLYATNMGADHLGDHVPADTMYAVE
                     GGANYGWPYCYQSGPRVLPDPKFNPRGRKFDCRKVPAAFAAFDAHGSPLGLEFFDRGH
                     GPELDGHFLVALHGSIKKGIMSGHRVVRVGGGEGDASHPARTFIDGFKSAGRVNGRPC
                     DIMRFGANGFLMTDDHAGVVYYVYQK"
BASE COUNT         1160 a         2510 c         2665 g         1213 t
ORIGIN      
        1 gcagcggcgc gtccgtcggt attggctgcc agctcgtccc gccgaagccg caacagctct
       61 ggttcgtcac gagcgtcaac tcgtcggtgc cgtcgaagtc tacgacgtgc atttcggtgc
      121 cgtggtagaa ggcgatcctc ttgccgtcgg gcgaccatgc ggggccggag gtctggccgc
      181 ggttcaggag ccgctgcgcg cccgtgccgt cgctcctcat gaataagatg tccgtgaagt
      241 tattcgagcg ctgcacgaac agtattttcg tcatgtcggg cgaccagagc ggcgacaggt
      301 agtgcgtcgg gccgtcgggc cccgtgccga aggcgacgag tttagagagg ccgctgccgt
      361 caacgttcat gacgtagatt tcgccgaagt ccttcgagaa gacgaagcgg gagccgtccg
      421 gcgaccagtc aacgtcgcgc acctgcgtcg ggctgccggg cagtttggcg tagcccgagc
      481 cgtcggcgtt ggcgaggtag atgaggcggt ctgtgccgac gtaggcgagg cgcgtgccgt
      541 cgggcgacca cgagaagccg ctgcggttgt cgaaggaggt gtcgatgacg ttgtcggcga
      601 gcgtgagccg ccccgagccg tcggcgttgg ccgcctcgat cacgtagtaa gcgtcgttgg
      661 ggtaggtggg ctgcctcttt tggacggtgt aggcgaggcg tgtgccgtcg ggcgaccagc
      721 gggggaagat gccgccctcg gcgccgggga acggggccgg cggcgcgctc gcgtccgcct
      781 tgaccgtgtg gacgccgtcg gaggcgaagg cgatcagacc gttcgtcttt accctgagcg
      841 gtgtggccgt gacggtgacg gtgacggccg ccgaattgtt gcccgcgtcg gggtccggcg
      901 tcgccgcggc cgcggccgtc gccgtgtggc cgaggacggc gccttcggcg acggccgcgt
      961 ccacggcggc gaccagaacg accgccgccg actgcccgac cgccagcgac gggaaggtca
     1021 ccgcccaact gccgccggag ttactacaga cgccgcccac tgcgccgcac gagacgaagg
     1081 tcgtccctgc ggggaggttg ccggagagcg tcgccgactc ggccgggccg ccgctgttgt
     1141 tggcgacgcg gaagaggtag ttgatccgcg cgccgggggc gaccttaccg tagctggcgg
     1201 cgcccgtgac ggacaggtcg gcgaagttca cctgcgtgct ctcggtcgcc gtgttgttgg
     1261 ccggcagggg gtcggcctcg ttgctcgtca cgctcgccgt gttgctgatg ttcctcacgg
     1321 cgggcggcag gacgacgatg gcgacggtcg tcgcagcgcc gccgttcagc gtgcccaggt
     1381 tgcaggtgac ggtggtcgtc cccgagcacg agccctggcc gggcgtggcg gagacgaagg
     1441 tcgccccggc gggcagcgtg tcggttagcg taacgccggc cgcgctcagc tcgccgttat
     1501 tagagacggt gaggatgtaa gtcaggttac tgccgaacgc gaccgggtcg ggcgagtccg
     1561 tcatggcgac ggccagttcg attgacgagc cgagcttggc gatgaaggcg tcgccgccgc
     1621 cgcggtacgc gttctgaaac ggggccgcga gggggaggtt gaacgagttg gtggcgccga
     1681 cgaagtaggt gttgcccgcg gcgtctacgg cgacggcatt gcccacatcg ttgccggcgc
     1741 cgccgtggta agtcgagaag gcgagcgccg tccccgaagg gttgagcttc gagacgaacg
     1801 cgtcgttgcc gccgccgcgt gtggtctgca ccgccccgac gagggggaag tcgttcgagg
     1861 aggtcagccc cgtgacgtga gcgttgccgg cagcgtccac ggcgatgccg aagccctgct
     1921 cggtcccgga gtcgccgccg aggtaggttg agtataggag ggtcgtgccg tcggcgctga
     1981 tccttgagac gaacgcgtcg tcgatgaagc tcggagggga aaacttcagc gggctggcga
     2041 gggggaagtt cgtggactgg gtgcggcccg tgaggtaggc gctgcccgca ggcccgaggg
     2101 cgacggcgcg cgcctcgtcg ttcgcgaagc cgccgagata ggtcgagtag acgagtccgg
     2161 cgcccgaagg attgagcttc gtcaggaagg cgtcagtgcc gccgcctttc gcgggctgga
     2221 gggcgccgag cacggggaag ttgttcgagg ccgtgtggcc tgtgatgtag gcgttgcccg
     2281 cggggtcggc cacgactccg gtggccatgt cgttctcgtt gccgccgagg aagcggaagt
     2341 ggacgacggc cgtgccttcg gggttgatct tgccggcgaa ggcgtcgctg ccgatgttcg
     2401 tgccggcgaa gacgtgggac gggacggcgc ggtcaacgac gatggcgccg acgctgttat
     2461 tgttgaggcc ggcgcttgcc tgcgcccagt tggccccgcc gtccgttgac ttgaagacgc
     2521 ccgagccgag cgtgccggcg tagaggacgg aagagttcgc ggggtcgatg gcgagcgcgt
     2581 cgatgcgcgg ggtgaatgtc gtcgcgccta tcgtggttct caggccggcg ccgacgacgt
     2641 tccactcgcc gccgccgttg gtggtcttca agagctggtt gccgccgaac acggtgaccg
     2701 ccgcgtagag cgtcgcgggg ttttgcgggt caatcaacag ggtcgtgacc gaggggggcg
     2761 tgaaggcgga gccgaggccc gtgttgagag gcgcccagtt ggccccgccg ttggtcgtct
     2821 tgaagatgcc ctggctcgtc ccggcgtaga ggacggaagg gttggtgggg tcgatgacga
     2881 ggtcgaacac ctgcggctcg aagctgaagg aggcggctcg gaggccgttg ttgatttgcg
     2941 tccagttgtc gccgccgttc gtgctcttgt tggcgccgaa gaaggaaccc gcgtacaggg
     3001 tggtcggggt cgccgggtcg ataacgatgg ttaagaggtt ctggtcaaag agtcctgtgt
     3061 tcttctcctc gtaggtctgc ccgccgtcgg tgctcttgta agcgccggcg aaggtggccg
     3121 cgtagacggt ctcgggggtc gtcgggtgaa cggcgaccca gttgacgaag cgcggcaagg
     3181 tcgagggctg ggcggcgccg gtcaggctcc agaggttgcc gccgtcggtc gtcttgaaga
     3241 cgccggacgc cgcgccggcg tagatcgtcg cggcgtccgt ggggtgggcg gcgaagaagt
     3301 tgacgcggct ggcggtgaac ccgttgtcgg cctcggccca actgccgccg ccgtcggcgc
     3361 tccggtagat ggggctgccg gccctcatgt cgaagacgcc ggccgaggcg atctcggagg
     3421 agtccgtgta gccggcgacg taggcgttgc ccgcggcgtc ggccgcgacg gcgagcacct
     3481 catcgttatt gttaccgccg agcaaggtgg aatagacgag cgccgatccc gtcgggttga
     3541 gcttggccac gtagccgtcg atcacgccga gcctcgactg ctgaagggcg ccggcagtaa
     3601 gcgggaagat gtcggaggtc gtgtagccca cgacgtaggc gttgcccgcc ggatcgacgg
     3661 cgacggcgtt gccgaagtcg tcgccgttgc cgccgaggta ggtcgcgtag actaagcctg
     3721 accccgtcgg gttgagcttg aggacgaagg cgtcgaagct gacatccttc gcggcctgga
     3781 tggggctggg gccggggaag tcggtcgaga aagtgtcgcc cgcgacgtag gcgttgcccg
     3841 cagcatcgac ggcgacgccg aagcccgcgt cggagtcgtt gccgccgagg taggtcgagt
     3901 agacgaggac ggggtcgatg acgagcgggc gactttggtc gtactcgccg acctcgaagg
     3961 cgacgcgatt ctccccgaga agcttgtagc cgccggccac ctcgcggcgc gcgccgtcga
     4021 tctcctgata gatgaatggc cgccgctggc gcacctcgcc gacctcggtg ccgagcacga
     4081 ggtcgccgcc cgcgccgagc gcgactgttt gcgcgccctc gaagtgaagc gcgatgcgcg
     4141 ccgggtcggc gcccgcgtcg agacggaagt cgtattcgag ctgccgctgc tggccgtagt
     4201 aaaccagatc gacgccgggg tagaccgact cgtacttgac cgcgccgaag ccggcgacat
     4261 ccgtcaccca gccgcgctcg tcggcgccga cgaagtagtt gctccggccc gacagttctt
     4321 cgacgccctc caacgcgggc gcgggattcg cgcccacgag accgacgcgc agcacggcgc
     4381 ccgaaggaga agacgccgcc gacggcgacg gagactcttc cgcggccccc gcctgcgacg
     4441 ctccctccgg cccgcccgcg tcggcggccg actcctcgcg gcctcccgcc tgacgcagac
     4501 tcagcaccgc ctccgtcggc gtcaggaaga gcgcgtaccc ttgcccgcgc gcgacgaatt
     4561 taacggccgc gtccgtctgc ccgcggttgg cctcgaagcg catcggcaac tgcccgtagg
     4621 cttccgtcag gcgcgcgcgc gtcgccgggt caacctgcgc gagtgcgtcg gggcgcgcgg
     4681 ccgcgcctcg tccttccgcc ccgcgactga gcgtctcgcg ccgcgaaact gcgcgcgcgc
     4741 tctcgctcac gtgcgccccg cgttccaaaa agagaaacgg cgacgcgagc agcgcgaccg
     4801 cgaacaacga aataacacag gcccggatga ggcggtgctt tccgaggggc gtagagcgag
     4861 gcattgcgag gttttccttt cgcgattagt gagacgggat ttgttgtcga aaggcaggga
     4921 gcaaaaccga ggccctctgc gggtgcggac gcattgagga gattgcaaag aatacttccg
     4981 tcgggcgcgg gaattctaca acgcgactcc cggcggcgac aagtcccgtc taaacatcgc
     5041 gccctctgct tcgtgcgaca cgcgcgcgtg ctatgatgcg cttccctttg aggcgggtgc
     5101 gaaacgtcgg ccgggaaaag cacccccatt cgggcggcgg gcgcgggacg ggaagcggcg
     5161 ccgaaaaggg cgatccgtat cgatctaaga tgggtaggct tcggaatatc tggagggggt
     5221 tgctgacggg tggcgcggcc ggcgtggcga cgctggcggc cgtaaacgcg gccgtggcgc
     5281 gcgaggcgcc ggagcccgag acgggcgcgc tcggcggcga ggccggcgcc tacgagtgga
     5341 gacacggcgg cgtcttcttc cgccacgccg gccccgacgc ggctccgccc gtcgtcttca
     5401 tccacggcgt cggcgccgga gcgcgctcct tcatgtggcg tcgcaacttc gagccgctct
     5461 ctcaggactt ccgcgtctat gcccccgacc tcctcggctt cggctactcc gacaagccct
     5521 cgaacgtgcc ctactccgca gacctctacg tcgagatgat cgttgacttc ctgcgcgacg
     5581 tggtgggccg tcgcgcgcac ctcgtcgccg gctcgctctc ggcggccttc gccgtccgcg
     5641 tcgctgatga gtaccccggc ctcgtcgatt cgctcgtgct catctccccg acgggcgtcg
     5701 gccagatgga ctcccggccc gacctgccgg gcgcggcctt ctacggcctg ctccactcgc
     5761 ctgtgctcgg cacctctttt tacaacgcca tgacgagcga gcgcggcatc cgcgactacg
     5821 cccgccggca gcttttctac gacccccgct tcgccacgcc gcgactcgtc gcccactact
     5881 acgccgtcag tcacctgccc ggcgcacact acgccgccac cgccttcctc tccggctacc
     5941 tgaactccga catccgcgag ccgttcgcgc ggctgcgcca accggcaacg ctcgtctggg
     6001 gccggcagga cgaggccaac cccgccaccc gaagccgcgc gctcctccac tcgaaccccc
     6061 gcgcccgcct ggaaatcttc gaccgctgcc ggcagatgcc ccaggaggaa cacgccgagc
     6121 gtttcaacgc gctgctgcgc gaagccttct ccgcggcacg ttgaaacgac acactaccgc
     6181 gagcggcggc gagcgggtgc ggctcgcccg cggcagtggt cacttgaacg cggcacacca
     6241 cattttactg atctctacca ttcaagagcc gcctgtcgta agatgcgccg acgatgacac
     6301 ccaagacaaa actgttccta ctccttgtca cactctggtt cgccgcaagc gccccggagg
     6361 ccacggctca ggcggcccgc actaccgcac gcgcgcaggt gcgcggtggt gcgagcgccc
     6421 cggcactaaa gccccatcgc atcaagctcg cgagcggcag gtcgttcgac ctcaacctgc
     6481 cggagggctt ccagatcagc gtcgcggcgc agggcctcaa gcgcgtgcgc ttcatggcgc
     6541 agagtcccga cgggcgcgtc ttcgtcactg acatgcacaa cctcgcagac aacgagcgcg
     6601 gcatcgtcta cgtcctcgac ggcttcgacc cggcccgcaa cgccttcgcg cgcgtcacaa
     6661 aatatctgac cggcctgcgt aaccccaaca gcgtcgcctt ccacaccgac cgcgacggcg
     6721 ccagttggct ctacctcgcg ctcaccgacc gtctgctgcg ctacagatac agaaacggcg
     6781 acaacgcgcc cacgggcgcg ccgcaggtgc tcgcacgctt ccccgactac ggactgaact
     6841 acaagtacgg cggctggcac ctgacgcgca ccatcgccgt cggcgcgaac ggcaggattt
     6901 acgtctcggt cgggagcagt tgcaacgcct gtgaggagaa ggaagaagta cgcgcaagca
     6961 tcatcgagat ggacgccgac ggcggcaacc agcgccaata cgcgcgcggc ctgcgtaacg
     7021 ccgtcggcct gcgcttcgcg ggcggccgac tctacgcgac caacatgggc gctgatcacc
     7081 tcggcgacca cgtccccgcc gacacgatgt acgccgtcga gggcggcgcc aactacggct
     7141 ggccttactg ctaccagtcc ggcccgcgcg tcctgcccga cccgaagttc aacccgcgcg
     7201 ggcgcaagtt cgactgccgc aaagtcccgg ccgccttcgc cgccttcgac gcgcacggct
     7261 cgcccctcgg cctggagttc ttcgaccgcg gccacggccc cgaactcgac ggccacttcc
     7321 tcgtcgccct ccacggctcg atcaagaaag gcatcatgag cggccaccgc gtcgtgcgcg
     7381 tcggcggcgg cgagggcgac gcctctcacc ccgcacggac ttttatcgac ggcttcaaga
     7441 gcgcgggccg cgtcaacggc cgcccgtgcg acatcatgcg cttcggcgcg aacggcttcc
     7501 tgatgacgga cgaccacgcg ggcgtcgtct actacgtcta tcaaaagt
//
LOCUS       DASVES010000036         4479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1323292, whole genome shotgun sequence.
ACCESSION   DASVES010000036 DASVES010000000
VERSION     DASVES010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323292"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VF240_01540"
     CDS             complement(<1..397)
                     /locus_tag="VF240_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173953.1"
                     /translation="MIKTVEWTDEGVRMIDQRVLPNEEKYLTLRSSEEVAEAIKKMVV
                     RGAPAIGVSAAMGIALGTKQSVGTSVADLEYDFDHNCDLLGRTRPTAVNLFWAIERMR
                     DCFQRAKAEGADAEAIKRRLVEESQAIFKE"
     gene            complement(633..1055)
                     /locus_tag="VF240_01545"
     CDS             complement(633..1055)
                     /locus_tag="VF240_01545"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX7173954.1"
                     /translation="MAKTICTILGALFILVGLVGFAAPGLGGFHLSLPHNLIHLISGA
                     VALYLGLKGTLSAARLFCIVFGIVYLLLGVAGYLAGADKPHTIPNLTHSGTDSNLLQV
                     IPSSLELASMDHGLHILLGLIFLIGGFLTKADVRRAVD"
     gene            1316..1606
                     /locus_tag="VF240_01550"
     CDS             1316..1606
                     /locus_tag="VF240_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173955.1"
                     /translation="MPRVLKWTGAAKRSAPGAAMGIWELSLLREPQRIGPAEAWRLVS
                     EGRRPVFLDTRNARHWGRSDEQIPGTLRIWREELEERIGEVPRGRPVVTYCA"
     gene            1817..3295
                     /gene="gatB"
                     /locus_tag="VF240_01555"
     CDS             1817..3295
                     /gene="gatB"
                     /locus_tag="VF240_01555"
                     /inference="COORDINATES: protein motif:HMM:NF004014.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7173956.1"
                     /translation="MMKDGWEAVIGLEIHAQLSTESKIFCGCSTRFGDEPNSNTCPVC
                     LGLPGSLPVLNRRAVELGARAALSLGLQINERSVFARKNYFYPDLPKGYQISQYDRPF
                     SERGVLEIMTAARDEGGHPRDWRPMTVRLTRMHLEEDAGKNVHEGLPETERFSYVDLN
                     RAGTPLAEIVTEPDFRTSWEAYDYVNHVRRALQWVGASEADMEKGNLRCEANVSVRRV
                     GEEKFGTKVELKNLNSVRYMQRAIEYELERQIALIERGGRVVQETLLWDERAGRTRPM
                     RSKEEAHDYRYFPEPDLPPLVVTADFIEETRASMPELPEARRRRFQDEYALSYEDATL
                     LTRDLALSDYYESAARSAGNPRGAANWIKSELLRVLDASGITPDRSPVTPEDLAALVR
                     LIDEGQISGTQGKDVLGEMFQTGKSAAAVVAERGIAQTSDAGEIERVINEVLAANPTQ
                     LEQYRGGKEGLFGFFVGQVMKASKGKANPKIVNDLLKVKLKA"
     gene            3292..4017
                     /locus_tag="VF240_01560"
     CDS             3292..4017
                     /locus_tag="VF240_01560"
                     /inference="COORDINATES: protein motif:HMM:NF005594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7173957.1"
                     /translation="MTPELKGKTAVVTGGTRGIGRAIAEALLREGMNVCVCARKAGEI
                     ERMVAELADVGEGGVSGAACDVRDADEVKALFAHAASEFGGVDVLVNNAGIGLRATVE
                     ETTVEQFRAVIETNLFGVFHCCREAIPMMRERGGGYIINLSSLAGANPHPSMATYNAS
                     KFALNGFSEALMQEVRHDRIKVSYIMPGSVNTEFGGDTPGPEKSWQLQPEDIARVVVD
                     LLRHDERSLPSRVEIRPSKPPKK"
     gene            4045..>4479
                     /gene="dtd"
                     /locus_tag="VF240_01565"
     CDS             4045..>4479
                     /gene="dtd"
                     /locus_tag="VF240_01565"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX7173958.1"
                     /translation="MLQRVTRASVRVEGETVGEIGAGLLVLLGVARDDTDADARYLLE
                     KITGLRVFEDAEGRMNLSVAEVGGALLVVSQFTLYGDVRRGRRPSWSDAAPPEVAEPL
                     YELFVGEARRTGLVVETGGFGRMMEVALINDGPVTILIDSRKH"
BASE COUNT          850 a         1381 c         1501 g          747 t
ORIGIN      
        1 cctccttaaa aatggcctgc gactcttcga cgaggcggcg cttgatggcc tcggcgtcgg
       61 cgccctcagc cttcgcgcgt tggaaacagt cgcgcatccg ctcaatcgcc cagaagaggt
      121 tgacggccgt cgggcgcgtc cgacccaaca ggtcgcagtt gtggtcgaag tcatactcaa
      181 ggtcggcgac agaggtgccg accgactgtt tcgtgccgag cgcgatgccc atcgccgccg
      241 agacgccgat ggccggcgcg ccgcgcacga ccatcttctt gatggcctcc gcgacctcct
      301 cgctggagcg cagcgtgagg tacttctcct cgtttggcag gacgcgctgg tcgatcatac
      361 gcacgccctc gtccgtccat tcgacggttt taatcattgc ggtaaacccc ctgtcagtat
      421 tttcgagaag atgtatacac taagtgaggc ggcggaacga gccggtgggc gtccgacatc
      481 ggaagacgcg tgtgatgcgg cgtcgctgtt gcctgacgac tggcagccga gttgaatttg
      541 agaaggatga tttaacttgt ggacatccgc tcgggcgtgg gcgaggcgag gtcgccccgc
      601 ccacatgccg agagagttcg tcgtcgccgg aatcaatcga cggcgcgcct gacatcggct
      661 ttcgtcagga aaccgccgat gaggaagatc aggcccagca ggatgtgcag gccgtgatcc
      721 atggaagcga gttcgagcga gctcgggatc acctgaagga gattggaatc tgtgcccgag
      781 tgcgtcaggt tggggatggt gtgcggtttg tcagccccgg cgagatagcc ggcgacgccg
      841 agcagcaggt acacgatgcc gaacacgatg cagaacaggc gcgccgccga cagcgtcccc
      901 ttcaacccaa ggtagagggc gacggccccc gagatgaggt ggatgaggtt gtgcggcaga
      961 ctcaggtgaa aaccgccgag gccgggggcg gcgaagccga ctaacccgac caggatgaac
     1021 aacgccccca agatggtgca gatcgttttc gccattactt taatactcct cctttggaga
     1081 gaaaaaggtg gacgatcaaa aagctcgccc cgtgtcactt gcgcgaacgg gccagccccc
     1141 gcgcggggcc ggacaagggc gatgtggatt cggacgaatt ctacaccaga aacgagtgcg
     1201 cgggagaggc atttgtcatt tgagcgtcac ggatttgtgg tttgttgagc gcgccgttgc
     1261 gcgaatgcta tactcgcccg ccgcctctat cagagaagtg tttacgtatt cacagatgcc
     1321 gcgcgtattg aaatggacgg gggcggcgaa gcgttccgcg ccgggcgcgg cgatggggat
     1381 ttgggagttg agcctacttc gggagccaca gcggatcggc ccggcggaag catggcgtct
     1441 cgtctcggag gggcgccggc ccgtcttcct cgacacgcgc aacgcccggc actggggacg
     1501 aagcgacgag caaatccccg gcacgctccg catctggcgc gaggagcttg aggagcggat
     1561 cggtgaggtg ccgcgaggcc gccccgtcgt cacctactgc gcctgacact acgagaactc
     1621 cagcacccgt gcggcgctga cgctcgaaga gtacgacttc gaagatattt acatcctcgt
     1681 cggcggcatc gacgcctggc gcgacgccgg gctgccgctg gaaccgaaga cggtgagcag
     1741 caagcagtga gcagtgagca gttgaaaaac tgtgtctgct tgacttctgc ttactgctca
     1801 ctgcttactg ctcactatga tgaaagatgg ttgggaagcc gtcatcggcc tggaaattca
     1861 cgcgcagttg agcaccgagt cgaagatttt ctgcgggtgc tcgacgcgct tcggcgacga
     1921 gccgaactcg aacacctgcc ccgtctgtct gggactgccc ggctctctgc ccgtcctgaa
     1981 ccggcgggcg gtggaactcg gagcacgcgc cgccctcagt ctcggcctcc agattaacga
     2041 gcgttccgtc ttcgcgcgca agaattattt ctaccccgac ctgcccaaag gctatcagat
     2101 ttcgcagtac gaccgcccct tctcggagcg gggcgtgctg gagatcatga cggccgcgcg
     2161 cgacgagggc gggcacccgc gcgactggcg gccgatgacc gtgcgtttga cgcgcatgca
     2221 tcttgaagaa gacgctggca agaacgttca cgagggattg cccgaaaccg agcgcttctc
     2281 ctacgttgat ctcaaccgcg ccggcacgcc gctggccgag atcgtcaccg agcctgactt
     2341 ccgcacgtcg tgggaggcgt atgactacgt caaccacgtg cgccgcgcgc tccagtgggt
     2401 cggggcgagc gaggccgaca tggagaaggg gaatcttcgt tgcgaggcca acgtctcggt
     2461 caggcgcgtc ggcgaagaga agttcggcac gaaggtcgag ttgaagaatc tgaactcggt
     2521 gcgctacatg cagcgagcca tcgagtacga gcttgaaagg cagatcgcgc tcatcgaacg
     2581 gggcggccgc gtcgtgcagg agacgctttt gtgggacgag cgcgcgggcc gcacgcgtcc
     2641 catgcgctcg aaggaggagg cgcacgacta ccgctacttc cccgagcctg atctgccgcc
     2701 cctcgtcgtc accgccgact tcatcgagga gacgcgcgcc tcgatgccgg agctgcccga
     2761 ggcgcgccgc cgccgctttc aggatgagta cgcgctctcc tacgaagacg cgacgctgct
     2821 cacgcgcgac ctcgcactct ccgactacta cgagtcggcc gcacgctcgg ccggcaaccc
     2881 gcgcggtgcg gccaactgga taaagtcgga gctgctccgc gtgctcgacg cctccggcat
     2941 cacgcccgac cgctcacccg tcacgcccga agacttggcg gcgctcgtgc gtctcatcga
     3001 cgaggggcaa atcagcggca cgcaggggaa ggacgtgctc ggcgagatgt tccagacggg
     3061 gaagagcgcc gcggcggtcg tcgccgagcg cggcatcgcg cagacgagcg acgcgggcga
     3121 gatcgaacga gtcatcaacg aagtgctggc cgccaacccg acccagttgg aacagtaccg
     3181 tggcggcaaa gaaggactct tcggcttctt cgtcgggcag gtgatgaaag cctcgaaggg
     3241 caaggccaat ccgaagatcg tcaatgacct gctgaaagtg aaactgaaag catgacgccc
     3301 gaactcaaag gcaagacggc cgtggtgacg ggcgggacgc gcggcatcgg tcgggccatc
     3361 gccgaggccc tgctgcgcga ggggatgaac gtctgcgtct gcgcccgcaa ggccggggag
     3421 atcgagcgca tggtcgcgga gcttgcagac gtgggcgagg gcggcgtgtc gggcgccgcg
     3481 tgcgacgtgc gcgatgccga cgaggtcaag gcgctcttcg cgcacgccgc ctcagagttc
     3541 ggcggcgttg acgtgctggt caacaacgcc ggcatcggcc tgcgcgccac cgtcgaggag
     3601 acgactgtcg aacagttccg cgccgtcatc gagaccaacc tcttcggcgt cttccactgc
     3661 tgccgcgagg cgattccgat gatgcgcgag cgtggcggcg gctacatcat caatttgtcg
     3721 tcactcgccg gggccaaccc gcacccatcg atggccacct acaacgcctc gaagttcgcc
     3781 ctcaacggct tcagcgaagc ccttatgcag gaggtgcgcc acgaccggat caaggtcagc
     3841 tacatcatgc ccggctccgt caacaccgag ttcggcggcg acacgcccgg ccccgaaaag
     3901 agctggcagc tccagcccga agacatcgcg cgcgtggtcg tcgatctgct caggcacgac
     3961 gaacgctctc taccgagccg cgtggagatc aggccgagca agccgccgaa gaagtgagca
     4021 tcgtccgcca taaatatgcg agccgtgtta cagcgcgtga cgcgcgcgag cgtgagagtc
     4081 gagggcgaga cggtcgggga gatcggggcg ggactcctcg tcttgctcgg ggtcgcccgc
     4141 gatgacaccg acgcggacgc gcgctacctc ttggagaaga taacggggct acgtgtcttc
     4201 gaggacgccg aggggcgcat gaacctttcg gtcgcggagg tcgggggcgc gctgctcgtc
     4261 gtctcgcagt tcacacttta cggcgacgtg cggcgcgggc ggcggccctc gtggagcgat
     4321 gccgcgccgc cggaagtggc cgagccgctt tacgaactgt ttgtcggcga ggcccggcgc
     4381 accgggcttg tggtcgagac cgggggcttc ggtcgcatga tggaagtcgc gctcattaat
     4441 gacgggccgg tgacgattct gatcgacagc aggaagcat
//
LOCUS       DASVES010000037         6452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_157155, whole genome shotgun sequence.
ACCESSION   DASVES010000037 DASVES010000000
VERSION     DASVES010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157155"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VF240_01570"
     CDS             complement(<1..874)
                     /locus_tag="VF240_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173959.1"
                     /translation="MSSRRRLSIAAGLPLVLAVFAVSVPAQQQKATSEPRPPIVSVEA
                     DPNVVTVCPDNGSMATTQVRLRARASSPDGNTLRYRPWTVGVGSIVGTGENVVWDLSG
                     VLPGTYTARVEVESGPDGNPLCEAFATVPVVVRACPPPRPYCPNVSISCPDTVAVGQP
                     VTFTATFSGGTPGVTPTYRWTVSEGSIISGQDTSSIQVGTVGLGGRAITAKVEVLGYN
                     LDCSASCTAQVPELLQARSFDDFSNIPRDDEKARLDNFAIQLQNEPGAAGHVIIYPAR
                     AERPGTARRRADRIR"
     gene            1185..2492
                     /locus_tag="VF240_01575"
     CDS             1185..2492
                     /locus_tag="VF240_01575"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7173960.1"
                     /translation="MPKLLRLTLPTLLLCAACLFAVAVRAQQQQQLVRETASPAAPGS
                     GQSNLAVDKRGRVYLSWIERLEKNRFALRFATREGAGAATGWSAPRTIAEGANWFVNW
                     ADFPSIAALPDGSLAAHWLVKSGPSTYAYDVHVSRSFDGGLSWSAPHVPHRDGTQTEH
                     GFVSMFPAKGGRLAAVWLDGREMKTTPTGDGHSSSTSGGVAAPKAAGDDHGHGQMTLR
                     YATLGRERKGFKIADEALLDARVCECCQTSAALTSEGPVVVYRDRSDTEMRDISIVRL
                     QRDGRWSAPATVHADNWRLDGCPVNGPSVAAAGRRVAVAWFTLADTNTPHVRLAFSRD
                     AGASFSPPVKVDDGDPVGRADVLLLDDGSALVSWIERTKGGAEVRARRVHPDGRRGDT
                     ITVAPSGAARSSGFPQMIRTRDRIIFSWTGTRVFTAEMPVPPR"
     gene            2619..3224
                     /locus_tag="VF240_01580"
     CDS             2619..3224
                     /locus_tag="VF240_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX7173961.1"
                     /translation="MVTPPRWTRQQRIDFADMDYPGTMYRAPAQNFRVPTSPRQIRYI
                     VIHITGGPAQDEGPAINTFRNGPASAHYIVNRQGHVTQFVRDAHIANHVDNINSETNR
                     DSIGIEHVNQWNQDGQLTPTDAQYGASANLVAWLCHQYQIPIVHDLARHAPGIRGHIE
                     EQPESGHVSCPNPAWDWDRYISMVQRVQVQSFASLIDSLAR"
     gene            3252..3893
                     /locus_tag="VF240_01585"
     CDS             3252..3893
                     /locus_tag="VF240_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7173962.1"
                     /translation="MKSEQAELRRSYDRVAEEYARQFRDELEQKPFDRKMLDWLAERV
                     GGAGPICDMGCGPGQVARYLHSRGAAACGVDLSPEMVRRARELNPEIEFSVGDMLALT
                     DVADGAWAGVAAFYSIIHIPRPSVVAALGELRRALRGGGLLLLTFHIGRETLHRDEWW
                     GEEVSLDFIFYETAEMKDYLDAAGFALEEVIERDPYPEVEYPSRRAYIFARNP"
     gene            4096..5067
                     /locus_tag="VF240_01590"
     CDS             4096..5067
                     /locus_tag="VF240_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7173963.1"
                     /translation="MFLFKSVLLCLACAQASLAWAPQASNSVVQTPRREIAITIDDLP
                     SAFQSDVRATRDETARILVALKRHRAPAIGFVNENKLHVAGEMDERIDLLRMWLDAGV
                     PLGNHTFSHVKFQDTPLWQYEDEVIRGEVVTRRLMRERGFTRLYFRHPYTSTGPTREA
                     REQFEAFLAERGYRVAPFTIEHADYLFNSVYVRALDAKDAATAARVRDAYVRHIDTAC
                     EYAERRSRELFGREIRQVLLIHANRINADALDATLARLEARGYSFVTLDAALEDNAYQ
                     TRDDYVGPLGISWLHRWSTALGAENKYREEPDAPKFIFDLLQAARGK"
     gene            5088..5699
                     /locus_tag="VF240_01595"
     CDS             5088..5699
                     /locus_tag="VF240_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173964.1"
                     /translation="MKRFRKVGMLLLSAAVVAAAALAVLQSNCPVEGGALSTQMRAFV
                     RLKNRTHLPRPEDFDPRVTLDALLLPGDDRGRWSEARAATVEGYVVGVATGGIEAANC
                     YSVTRRDTHVYVARRPDAPTSEAVVAEVTPRVREWATLAGDDWSEESLRRRLVGRRCR
                     VEGWLLYDTEHDGESENTAPGRALNWRATAWEIHPLTRVEVLE"
     gene            5696..6103
                     /locus_tag="VF240_01600"
     CDS             5696..6103
                     /locus_tag="VF240_01600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7173965.1"
                     /translation="MSASELEYRPAGAQDYEVVRQFLCEVGWQRRVGDPERFRAIVEG
                     AQRTVVAFDGGRVVGFARALCDGVSNGYISMVAVAVDMRGRGVGRELVRRLIGEDEGI
                     TWVLRAGHGSEGFWSVMGFEASEVAMERVRKVE"
     gene            6120..>6452
                     /locus_tag="VF240_01605"
     CDS             6120..>6452
                     /locus_tag="VF240_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173966.1"
                     /translation="MLRTRAACVLLLCSLLSPCPAWAQQPSPAVTNDPVERIKDEGLN
                     RSQVMQTLSYLTDVIGPRLTNSPQMKRANEWTRDTLTKWGLQNAKLEAWGPFGRGWSL
                     KRFSAEVIE"
BASE COUNT         1116 a         2130 c         2171 g         1035 t
ORIGIN      
        1 cgcggatgcg gtcggcgcgg cggcgtgccg tgccgggccg ctcggcgcgc gccgggtaga
       61 tgatgacgtg gccggccgcg cccggctcgt tctggagctg gatggcgaag ttgtcgaggc
      121 gggccttctc gtcgtcgcgc gggatgttgc tgaaatcgtc gaaggagcgc gcctgtaaaa
      181 gctctggcac ctgcgccgta cacgaggcgc tgcaatcgag gttgtagccg agcacttcga
      241 ccttcgccgt gatagcgcgc ccgccgaggc ccaccgtgcc cacctggatg gaggacgtgt
      301 cctgccccga gatgatggag ccctcggaga ccgtccagcg gtaggtgggc gtgacgccgg
      361 gcgtgccgcc cgagaaggtg gccgtgaagg tgacgggttg gccgacggcg accgtgtcgg
      421 gacaggaaat cgagacgttc gggcagtagg gccgcggcgg cgggcaggcg cgaacgacga
      481 cgggcaccgt ggcgaacgcc tcacacagag ggttgccgtc ggggccgctc tccacctcca
      541 cgcgcgccgt gtaggtgccg ggcagcacgc cagagaggtc ccagacgacg ttctcgcccg
      601 tgcccacgat actccctacg cccaccgtcc atgggcggta gcgcagcgtg ttgccgtcgg
      661 ggctgctggc gcgcgcgcgc aggcgaacct gcgtggtcgc catcgacccg ttgtcggggc
      721 agacggtgac gacgttgggg tcggcttcga ccgaaacaat cggcgggcgc ggctcggacg
      781 tcgccttctg ctgctgcgcc gggacgctga cggcgaacac ggccagcacc aacggcaggc
      841 cagcggcgat tgataagcgc ctacgtgaag acatttctca ctccttagaa ttgatgtgtc
      901 cggccccgag cggtcgctag ggttataaca cgtccgcgcc cgccccaacc cgattttgaa
      961 gttcttccgg ccgcccacaa agggctgtac ttctgttgaa acgaacaatt acgcccgttt
     1021 tccaacgaaa acgcgtaatt aaaagcacgc cgtgggcgcg agcctgtttg attttttccg
     1081 ccgggtgtaa tcaaatcctc tcgcagtgca cgcgccgccg agccggacac cggcaagcag
     1141 ccgccgagcc gggtcaacga ttcaacgaat acgggatgac cagaatgccg aaactactcc
     1201 gcctcacgtt gccgacgctg ctcctgtgcg cggcgtgtct gttcgccgtc gccgtccgcg
     1261 cgcagcagca gcagcagttg gtgcgcgaga ctgcctcgcc cgccgcgccc ggcagcgggc
     1321 agtcgaacct cgcggtagac aagcgcgggc gcgtctacct cagttggatc gagcgactgg
     1381 agaagaaccg cttcgccctc cgcttcgcga cgcgcgaggg cgcgggcgcc gccaccgggt
     1441 ggtctgcgcc gcggacgatt gccgagggcg ccaactggtt cgtcaactgg gccgacttcc
     1501 cctccatcgc cgcgctcccc gacggctcgc tcgccgccca ctggctcgtc aagagcggcc
     1561 cctcgaccta cgcctacgac gtacacgtct cgcgctcctt cgacggcggt ctctcatggt
     1621 cagctcccca cgtcccacac cgcgacggca cgcagaccga gcacggcttc gtctccatgt
     1681 tccccgccaa aggcggacgc ctcgccgccg tctggctcga cggtcgcgag atgaaaacca
     1741 cccccactgg cgacggccac agcagcagca ccagcggcgg cgtggctgcg ccaaaagcgg
     1801 cgggcgacga ccacggccac ggccagatga ctttgcgcta cgcgacgctc ggccgcgagc
     1861 ggaagggatt caagatcgcg gacgaggccc tgctcgacgc gcgcgtctgc gagtgctgtc
     1921 agacttcggc cgcccttact tccgaaggcc ctgtcgtcgt ctaccgcgac cgctccgaca
     1981 cggagatgcg cgacatctcc atcgtccgcc tccagcgcga cgggcgttgg agcgcgccgg
     2041 cgaccgtcca cgccgacaac tggcgtctcg acggctgccc cgtcaacggc ccctcggtcg
     2101 cggccgccgg ccgccgcgtc gccgtcgcgt ggttcaccct cgccgacacc aacacgcccc
     2161 acgtccgcct cgccttctcg cgcgacgccg gggcgagttt ctccccgccc gtcaaggttg
     2221 acgacggcga ccccgtcggc cgcgccgacg ttctcctgct cgacgacggc agcgccctcg
     2281 tcagttggat cgaaagaacc aaaggcggcg ccgaagtccg cgcccgccgc gtccaccctg
     2341 atggccggcg cggtgacacc atcaccgtcg ccccctccgg cgccgcccgc tccagcggct
     2401 tcccccagat gattcgcaca cgcgaccgca tcatcttctc gtggacgggc acgcgcgtct
     2461 tcaccgccga aatgcccgta ccgccacgtt gaaagcgcag tcgtcaatac catgtacggg
     2521 tgacacgcaa aacgcgcgcg cgcgatctgt gccggttgcg tataatcggt gatcaggccc
     2581 tcgcacgacc cgacactttc cgcgagagag gaacatcaat ggtcacaccc ccgcgttgga
     2641 cacgccagca gcgcatcgac tttgctgaca tggactatcc gggaacgatg taccgggcgc
     2701 ccgctcagaa cttccgagtg cccacgtcgc cgcggcagat ccgttacatc gtcattcaca
     2761 tcaccggggg gccggcacaa gacgaggggc cggcaatcaa caccttccgc aatggccccg
     2821 cgagcgccca ctacatcgtc aaccggcagg gccacgtcac acagttcgtc agggacgcgc
     2881 acatcgcgaa ccatgtagac aacatcaact ctgaaaccaa ccgcgacagc atcgggatag
     2941 aacacgtcaa ccagtggaat caggacggcc aactcacgcc gaccgacgcg cagtatggcg
     3001 cttccgccaa tctggtcgcc tggctgtgtc atcagtatca aattcccatc gtccatgatc
     3061 tcgcccgcca cgcccccggc atacgcgggc acatcgaaga gcagcccgaa tcgggtcacg
     3121 tcagttgccc caaccccgca tgggattggg atcgatacat ctctatggtt cagcgcgttc
     3181 aggttcagag tttcgcatca ctgatcgatt cacttgctcg ttgaccgagc gacacgagga
     3241 acgtgttagg aatgaagagt gagcaggcag agttgcggcg cagttacgac cgcgtggcgg
     3301 aggagtacgc gcggcagttc cgcgacgagt tggagcagaa gccgttcgac cggaagatgt
     3361 tggactggct ggcggagagg gtcgggggcg cggggccgat ctgcgacatg gggtgcgggc
     3421 cggggcaggt ggcgcgctac ctgcatagtc gcggggcggc cgcttgcggg gttgacctgt
     3481 cgccggagat ggtgaggcgg gcgcgcgagt tgaacccgga gattgagttc agcgtgggcg
     3541 acatgctcgc gctcacggac gttgcggacg gcgcgtgggc gggcgtcgcc gccttctact
     3601 cgatcatcca cattccgcgc ccgtcggtcg tcgcggccct cggcgagctg cggcgggcgc
     3661 tgcgcggggg cggacttctc ctgctcactt tccacatcgg gcgggagacg ttgcaccgcg
     3721 acgagtggtg gggcgaggag gtgtctctgg atttcatctt ctacgagacg gcggagatga
     3781 aggattatct cgacgccgcg ggcttcgcgc tggaagaagt gatcgagaga gacccgtacc
     3841 cggaggtgga gtacccgagc cggcgcgcgt acatcttcgc gaggaatcca tagggtgatg
     3901 atgattgcgc ctccagcgtg agtacatcgg ctccttaaac agtaccgcga gcggtagcga
     3961 gcgggtatga ctgtgatgag aggcccgcat cgcacacgcg agacccgcga ggcttccgct
     4021 cgcggtactc tgtgcgcgcc ggcggatgaa gtctcacgcc ggaggcgcaa agaagaggga
     4081 gaaaaagttg tgagggtgtt tctgtttaag tccgttctgt tgtgtctcgc gtgcgcgcaa
     4141 gcttcgcttg cctgggcgcc acaggcgtcg aactccgtcg tccagacgcc gcgccgcgag
     4201 atcgccatca ccatcgacga cctcccttcg gccttccaga gcgacgtgcg ggcgacacgc
     4261 gacgagacgg cgcgcatcct cgtcgcgctc aagcgccatc gcgcgccggc catcgggttc
     4321 gtcaacgaga acaagctcca cgtggcgggc gagatggacg agcgcatcga cctgctccgc
     4381 atgtggctcg acgccggcgt gccgctcggc aaccacacct tctcccacgt taaatttcag
     4441 gacacgccgc tttggcaata tgaggacgaa gtcatccgcg gcgaggtcgt cacgcgccgg
     4501 ctgatgcgcg agcgcggttt cacgcgactc tacttccggc acccgtatac gagcaccggg
     4561 ccgacgcgcg aggcccgaga gcagttcgag gcgttcctcg ccgagcgcgg ctaccgcgtc
     4621 gcgcccttca ccatcgagca tgccgattac ctgttcaact cggtctacgt ccgcgccctc
     4681 gacgcgaagg acgccgcgac ggccgcgcgc gtccgcgacg cctacgtccg gcacatcgac
     4741 acggcctgcg agtacgccga gcggcgctcg cgcgaactct tcggccgcga gatcagacaa
     4801 gtcctcctca tccacgccaa ccgcatcaat gccgacgcgc tcgacgcaac gctcgcccgg
     4861 ctcgaagcgc gcggctactc cttcgtcacg ctcgacgccg cgctcgaaga caatgcttac
     4921 cagacgcgcg acgactacgt ggggccgctc ggcatctcct ggctccaccg ctggagcacg
     4981 gccctcggcg cggagaacaa gtatcgcgag gagcctgatg cgccgaagtt catcttcgat
     5041 ctgcttcagg cggcgcgcgg caagtgagcc gcgaccgagc gtgcgccgtg aaacgattcc
     5101 gaaaagtcgg catgctcctg ctgtcggccg cagtggtcgc ggcggccgcg ctcgccgtcc
     5161 tccagtcaaa ctgtcccgtg gagggcgggg cgctctccac acagatgcgc gccttcgtgc
     5221 gtctcaagaa ccgcacacac ctgccgcgcc ccgaagactt cgacccgcgc gtgacgctcg
     5281 acgcgctgct ccttccgggc gacgaccgcg ggcgctggtc ggaggcgagg gcggccacgg
     5341 tcgagggcta cgtggtcggc gtcgcgacgg gcggcatcga agcggcaaac tgttattctg
     5401 tgacgcggcg cgacacgcac gtctatgtgg cgcggaggcc ggacgcaccg acaagtgagg
     5461 ccgtggtagc ggaggtcacg ccacgcgtca gggagtgggc cacgctcgcg ggcgatgact
     5521 ggtcggagga gtctctgcga cgcaggctcg tcggccgccg gtgtcgcgtg gaggggtggc
     5581 tgctctacga cacggagcac gacggcgagt cggaaaacac ggcgccgggc cgcgcgctga
     5641 actggcgggc gactgcctgg gagattcacc cgttgacgcg cgtcgaggtg ttagagtgag
     5701 cgcgagcgag ttggagtaca ggcccgcggg cgcgcaagac tacgaggtcg tccgacagtt
     5761 tctctgcgag gtaggctggc agagacgcgt gggcgatccc gagaggttca gggcgatagt
     5821 ggagggcgcg caacgaacgg tcgtggcctt cgacggtggg cgcgtcgtcg ggtttgcgcg
     5881 ggcgctgtgc gacggagtgt cgaacggcta catctcgatg gtcgcggtgg ccgttgacat
     5941 gcgtgggcga ggcgtcgggc gcgagctggt gcggcgtctc atcggagagg atgaggggat
     6001 aacgtgggtg ttgcgcgcgg ggcatgggag cgaaggcttc tggagcgtca tggggtttga
     6061 agcgtcagaa gtcgccatgg aaagagtcag gaaggttgaa tgaaagctta aggagataca
     6121 tgctacgaac acgggccgct tgcgttttac ttctctgctc tcttctgtcg ccgtgccccg
     6181 cgtgggcgca gcagccgtcg ccggctgtta cgaacgaccc ggtcgagcgg atcaaggacg
     6241 aagggctgaa ccgctcgcag gtgatgcaga ccttgtcgta tctgaccgat gtcatcggcc
     6301 cgcgcctcac gaactcgccg cagatgaagc gcgccaacga gtggacgcgc gacacgctga
     6361 cgaagtgggg cttgcagaac gcgaagctcg aagcgtgggg gccgttcggg cgtggatggt
     6421 cgttgaagag attctcagcc gaggtcatcg ag
//
LOCUS       DASVES010000038         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1711575, whole genome shotgun sequence.
ACCESSION   DASVES010000038 DASVES010000000
VERSION     DASVES010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711575"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..572)
                     /locus_tag="VF240_01610"
     CDS             complement(90..572)
                     /locus_tag="VF240_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173967.1"
                     /translation="MNERTKAALIGGAVAGILSAIPILNNCCCLWAICGGILAILVYT
                     KSTKAAMTPGDGAMLGVVAGGIGVAIYLAIGLPVFLLIGAASITSQMSQAGVDIPFSG
                     TILAIIVALIFAIGVFAFTVLGGLIGAAIFGKGGAGAPPPPPPPNFGGPAGGTYGAGS
                     "
     gene            complement(696..1253)
                     /locus_tag="VF240_01615"
     CDS             complement(696..1253)
                     /locus_tag="VF240_01615"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX7173968.1"
                     /translation="MPSVITDYVPQFVPEGGVPPRTRAGLLAWGITLLAASLFVALIL
                     LAPLLRARGAMAWSQIIYMALGSLCHQMPERSFQFEGFPLAVCARCWGLYVGALAGLL
                     LYPFVRSLSRTDAPPRAWLLLAALPSALDFLLGISGAWENTHASRFLTALLLGGVAAF
                     YVVPGLVDLSSQSFRQFFGNLYSNG"
     gene            complement(1310..2350)
                     /gene="glpX"
                     /locus_tag="VF240_01620"
     CDS             complement(1310..2350)
                     /gene="glpX"
                     /locus_tag="VF240_01620"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX7173969.1"
                     /translation="MAPELNSVNLSLDFLSVCEAAAIASARTMGQGDRKYSDHVAVEA
                     MREKMDAVPMRGRIVIGEGERDEAPMLYIGEEVGQGVRDRDNPEYPEVDIAVDPLEGT
                     NLCALGANNAIAVLAASERGGLLNAPDIYMDKIVVGPSSRGAIDIDAPVKDNLKGIAR
                     RLGRDVEDLTVIVLDRTRHKKLIQDVRAAGARIRLISDGDLSAGISAAVAGTNIHALM
                     GIGGAPEGVITAAALKCLNGEIQARLVYDPERLGVDMGMVPAADEVLGRLREMGITDP
                     NKIYDVNDLAPGKKVIFAATGVTDGALLKGVRLFGAGKRTHSLIMTTESRHIRFIDTV
                     HVEGGPDTVIRF"
BASE COUNT          468 a          793 c          830 g          395 t
ORIGIN      
        1 gtcgcgtccg cgtctcgacc gacgcggacg cgacaaaaga cgagcgcgct cgcgcgggcc
       61 atcagaagtt ccgcgccgag tgagatgtct tagctgccgg cgccgtaggt gccgccggcc
      121 gggccgccga agttgggcgg aggcggaggc gggggcgcgc cggcgccgcc cttgccgaag
      181 atcgcagccc cgatcaagcc tccgagcact gtgaaggcga agacgccaat ggcgaagatc
      241 aacgcgacga tgatggcgag gatggtgccg gaaaacggta tatcgacgcc cgcctgtgac
      301 atctgactcg tgatcgacgc cgcgccgatc aggagaaata cggggaggcc gatggcgagg
      361 tagatcgcca cgccaatgcc gcccgcgacg acgccgagca tcgcgccgtc gcccggcgtc
      421 atcgccgctt tcgtgctctt cgtgtacacc aagatggcga ggatgccgcc gcagatggcc
      481 cacaagcaac aacaattgtt gaggatcggg atagcggata agatgcccgc gacggctccg
      541 ccgatgagcg cggctttggt tctctcgttc atgctttacc taccttcttc aattttgggt
      601 taaagcgttg gctggagaaa tatgactttg gcgaatggcc cgacgcgccg gccgccgcca
      661 gtcgtcacct gaagcacgag acaccctcgc gctctctatc cgttcgagta aagattgccg
      721 aagaattgcc gaaagctctg gctgctcagg tcaactaaac ctggcacgac gtagaacgcg
      781 gcgacgccgc cgagcagcag cgccgtcaga aagcgcgagg cgtgcgtgtt ttcccacgcg
      841 cctgagattc cgagcaggaa gtcgagggcg ctcgggagcg ccgccagcaa caaccacgcc
      901 cgcggcggcg cgtcggtgcg cgaaagcgaa cgcacaaacg ggtagagaag caaccccgcg
      961 agcgcgccga cgtacagccc ccagcagcgc gcgcacaccg ccagcgggaa cccctcgaac
     1021 tggaacgagc gctcgggcat ctggtggcac agcgatccta aagccatgta gatgatctgc
     1081 gaccaggcca tcgccccgcg cgcgcgcagc agcggcgcga gcaagatcag cgccacgaac
     1141 agcgacgccg cgagcagcgt gattccccac gccaaaagcc ccgcgcgtgt cctcggcggc
     1201 acgcccccct cgggcacgaa ctgcggaaca taatcggtga tgacggaagg catttcaaga
     1261 ttgcgggccg cggatttgaa aataggtcgc ctctcatcaa atccgcaatc taaaatctga
     1321 tgaccgtgtc agggccgccc tcgacgtgga cggtgtcgat aaagcggatg tggcgcgact
     1381 cggtggtcat aatcagcgag tgggtgcgct tgccggcgcc gaagaggcgg acgcccttga
     1441 ggagcgcgcc gtcggtgacg ccggtggcgg cgaagatgac cttcttgccg ggggcgaggt
     1501 cgttcacgtc atatatcttg ttggggtcgg tgatgcccat ctctcgcaga cgaccgagca
     1561 cctcgtcggc cgccggcacc atgcccatgt caacgccgag gcgctccggg tcgtagacga
     1621 ggcgcgcctg tatctcgccg ttcagacact tcagggccgc ggccgtaatc acgccttcgg
     1681 gcgcgccgcc gatgcccatc agcgcatgga tgttcgtgcc cgccacggcc gccgagatgc
     1741 cggccgagag gtcgccgtcg ctgatgaggc ggatgcgtgc gcccgcggcg cgcacgtcct
     1801 gaatgagctt cttgtggcgc gtgcggtcga ggacgatgac ggtcaagtct tccacgtcgc
     1861 gccccaaacg gcgggcgatg cccttgaggt tgtctttgac gggcgcgtcg atgtcgatgg
     1921 ccccgcgcga ggaggggccg acgacgatct tgtccatgta gatgtcgggc gcgttgagga
     1981 ggccgccgcg ctcggaggcg gccagcaccg cgatggcgtt gttggccccc agcgcgcaga
     2041 ggttggtgcc ttcgagcgga tcgacagcga tatccacctc ggggtattcg gggttgtcgc
     2101 ggtcgcgcac gccctgaccc acctcctcgc cgatgtagag catcggggcc tcgtcgcgct
     2161 cgccctcacc gatgacgatg cggccgcgca tcggcacggc gtccatcttc tcgcgcatcg
     2221 cttcgacggc gacgtggtcg gaatacttgc ggtcgccctg ccccatggtg cgcgccgagg
     2281 cgatggccgc cgcctcgcac acgctcagga agtcgaggga aaggttgacg ctgttcaact
     2341 ctggggccat ctgcctacac tccgcttcgg aacaaaaaga ttgccgtgaa aaactcccgc
     2401 cggaaaatgc gctggcaagt gggaaggctc ggtgcatact atcagagaga gcgccgaaat
     2461 tgtagggcca atcgggccgg gaatcg
//
LOCUS       DASVES010000039         4678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_349103, whole genome shotgun sequence.
ACCESSION   DASVES010000039 DASVES010000000
VERSION     DASVES010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349103"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VF240_01625"
     CDS             complement(<1..693)
                     /locus_tag="VF240_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7173970.1"
                     /translation="MNIAIRQPALIVALLFGVLLAPASAPAQANNRVEQLERAASLIG
                     GGQLAEAERQLNQILRSAPNEAAALNLLGALRAKQGRLDEAEALFSRSVRGDNRLTGA
                     HMNLAYLYLLKREPEKSAASLREVLRLEPSNADAAHRLAWLLHSQGRFDECIGFVEKL
                     KQSQALTAPVLALLADAYLKKGAAGEAVAALKKAIELQPGEPSYHFALGAAWLRQPPD
                     LLGAEQSFRQFLT"
     gene            complement(705..2408)
                     /locus_tag="VF240_01630"
     CDS             complement(705..2408)
                     /locus_tag="VF240_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX7173971.1"
                     /translation="MLLRTIGLLAASIVWPLSSPPPATGNQPAGPRFTDVTAQAGITF
                     KHVSTPEKRYIVESMSGGVALIDYDNDGLLDIYLVNSLTVDLVKSKGKTKSALYRNRG
                     DGTFADVTEKSGAGDIGWGMGAAVGDYDNDGFQDIYVTCVGPNHLLRNNGDGTFADVT
                     VKAGVGDPRWSAGASFVDYDADGRLDLFVSNYVVFDFNNLPEFGKDKTCQFKGLAVQC
                     GPRGMPGDGDSLYRNNGDGTFTDVTKKAGVSDPNGYYGMGVISSDFDEDGLIDIFVAN
                     DSTPNFLYKNQGDGTFKEVGFLSGTALNENGAAQGCMGVTVADYDHDGRLDLFITNFD
                     DDYNTLYRNDGRNSFTDASYAAKIAAVSLPYVGWGTKFFDYDNDGWVDLFVANGHVYP
                     QIKTFHQRNFVHRNNRDGTFTETAEQLGAPFAEKHAARGAAFGDIDNDGDVDVVVNNL
                     GSAPQLLRNDGGNAGNSVLIKTVGVKSNRDGIGARVKVVSGDLVQKDEVRSGDSYLSQ
                     SDLRLHFGLERRTKIDLIEIRWPSGAVDKITDARPNRVLTVKEGQGVVEQKDFKVVPR
                     R"
     gene            complement(2432..>4678)
                     /locus_tag="VF240_01635"
     CDS             complement(2432..>4678)
                     /locus_tag="VF240_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7173972.1"
                     /translation="FAESTEAVKEAMYFASALLPLLLLLPHFQAPSDSFRKHHEAAEA
                     HRRAGNTAAAEAEYAAILAGAYPALGRIYTAQSNYREAVVALEAAVPYRPDSPEVLVG
                     LAVAYFYAGQYQKAVEPLNRVLAHDPRSAPARHMLGKTHFMMGEFAGAARELEAALAL
                     KPDDYDAAYTLGLAYLKQRQFAGARRVYERMVAQLGDRPQLRVLIGRAYRETGFLAEA
                     IEEFKRAVALDPRFPRVHYHLGRTYLLKDGAARLGDATKELQAELAAHPDEFLANYYL
                     GIIHTSEGKWPPAVPLFEKASRIQPRNPDPHFFLGQAYQNLGKYEQAIAAFRKAIALN
                     PSLGHNDYQVTNAHYRLGQTLLKAGRTEEGERELQTAADLKSKAFKRDGAKLDAFLND
                     AEADEQSKFPELVSAPGMVAGSVAPDAREREVLAADASFYEKVIAAAHNNIGLLRAER
                     QDFRAAAAQFSSAARLNPQHEGLDYNLGLAYFKSESFKDAVAPLENEIKARPSNLQAK
                     RLLGLSYFMLENYAGASALLAEVVAARPDEAALYYPLALALGKVGKTQEAERVTRQMV
                     AMGGQSPQVHILLGRAYYEQNDAAKALGELRTALALDDKVLLAHFYAGLIYVKTGKLD
                     EAAREFEAELVLNPGDVQARYHLGFVLLARQETERGIRLMREVIGARPDFANARFELG
                     NALLKRGDARGAVESLEAAAKLAPGEAHIHYQLGRAYIAAGRQADGEGQLEIARQLKE
                     KALRQANQ"
BASE COUNT          741 a         1455 c         1596 g          886 t
ORIGIN      
        1 ggtgaggaac tgtcggaaag attgttcggc ccccagaagg tctggcggct ggcggagcca
       61 tgcggcgccg agggcgaagt ggtatgacgg ctcgccgggc tggagttcga tggctttttt
      121 aagcgccgcc acggcctcgc ccgctgcgcc ttttttcagg taggcgtcgg cgagtaaggc
      181 gagcacgggg gccgtgagcg cttgcgactg cttcagcttt tcgacgaagc cgatgcactc
      241 gtcgaagcgg ccctgcgaat gcaacagcca ggcgagccgg tgggcggcgt cggcgttgga
      301 cggttcgagg cgcagcactt ctcgcaacga cgcggccgac ttctccggct ccctcttgag
      361 cagatagaga taggccagat tcatgtgcgc gcccgtcagg cggttgtcgc cgcggacgga
      421 gcgcgagaag agggcttcgg cctcgtcgag tctgccctgc ttggcgcgga gcgcgccgag
      481 caggttgagc gccgccgcct cgttcggcgc ggagcgcagg atttgattca actgccgctc
      541 ggcctccgca agttggccgc cgccgatgag cgacgccgcg cgctccagtt gttcgacgcg
      601 attattggcc tgcgccggcg cgctcgccgg ggcgagcaga acgccgaaga ggagcgcgac
      661 aatcagcgcc ggctggcgga tagcaatgtt cataggcaga cggcttagcg ccgggggacg
      721 accttaaaat ctttctgctc gacgacgccc tgaccctctt tgacggtgag cacacggttc
      781 ggccgcgcgt cggtaatctt atcaaccgcg ccgctcggcc agcgaatctc gatgaggtcg
      841 atcttcgtgc gccgctccag accgaagtgc agccgcaggt cgctctgcga cagatagctg
      901 tcgccgctgc ggacttcgtc cttctgcacg aggtcgccgg agacgacctt cacgcgcgcc
      961 ccgatgccgt cgcggttgct ttttacgccg accgttttta tcaggacgga gttgccggcg
     1021 ttgccgccgt cgttgcgtag cagttgcggg gcgctgccca gattgttgac gaccacgtcc
     1081 acgtcgccgt cgttgtcgat gtcgccgaag gcggccccgc gcgccgcgtg tttctcggcg
     1141 aagggcgcgc cgagttgctc ggccgtctca gtgaaggtgc cgtcgcggtt gttgcggtgg
     1201 acgaagttgc gctggtggaa ggtttttatc tgcgggtaga cgtggccgtt ggcgacgaac
     1261 agatcgaccc agccgtcgtt gtcgtagtcg aagaacttcg tgccccagcc gacgtaaggc
     1321 aaactcaccg cggcgatctt ggccgcgtag gaggcgtcgg tgaaggagtt tcgcccgtcg
     1381 ttgcggtaga gcgtgttgta gtcgtcgtcg aagttggtga tgaagagatc gaggcggccg
     1441 tcgtggtcgt agtcggccac cgtcacgccc atgcacccct gggccgcgcc gttctcgttg
     1501 agggcggtgc cggagaggaa gccgacctct ttgaacgtgc cgtcgccctg attcttatag
     1561 aggaagttcg gcgtcgagtc gttggcgacg aagatgtcga tgaggccgtc ttcgtcgaag
     1621 tcgctgctga tgacgcccat gccgtagtag ccgttcgggt cggagacgcc ggccttcttc
     1681 gtcacgtcgg tgaaggtgcc gtcgccgttg ttgcgataga gcgagtcgcc gtcgccgggc
     1741 atcccgcgcg ggccgcactg cacggcgagg cccttgaact ggcacgtctt gtccttgccg
     1801 aactcgggca ggttgttgaa gtcgaagacc acgtaattgg agacgaagag gtcgaggcgg
     1861 ccgtccgcgt cgtaatcgac gaacgacgcg ccggccgacc agcgcgggtc gcccacgccc
     1921 gccttcacgg tcacgtcggc aaacgtgccg tcgccgttgt tcctgagcag gtggttcggc
     1981 ccgacgcagg tgacgtagat gtcctggaaa ccgtcgttgt cgtaatcgcc gacggcggcg
     2041 cccatccccc agccgatgtc gccggcgccc gacttctccg tcacgtcggc aaacgtgccg
     2101 tcgccgcggt tgcggtagag cgcgcttttc gtcttcccct tcgacttcac gagatcgacg
     2161 gtcagggagt tgacgaggta gatgtccagc aggccgtcgt tgtcatagtc gatgagcgcc
     2221 accccgccgc tcatcgattc gacgatatat ctcttctcgg gcgtggagac gtgcttgaag
     2281 gtgatgcccg cctgcgccgt gacatccgtg aagcgggggc cggccggttg attgccggtt
     2341 gcaggcgggg gtgatgaaag cggccaaacg attgacgccg cgagcagacc tatcgtcctt
     2401 aagagcaaat ggcgcgacct cggttctcgg tttattgatt ggcctgacgc agagctttct
     2461 ctttcaactg ccgggcgatt tcgagctgac cctcgccgtc cgcctgccgg ccggcggcga
     2521 tgtaggcgcg gccgagttga taatgaatgt gggcctcgcc gggcgcaagc ttcgcggccg
     2581 cctcaagact ctcgaccgcg ccgcgcgcgt cgccccgttt cagcagcgcg ttgcccagct
     2641 cgaagcgcgc gttggcgaag tcgggccgcg cgccgatgac ctcgcgcatc aaccggatgc
     2701 cgcgctcggt ctcctgacgc gcgagcagca cgaagccgag atgatagcgg gcctgcacgt
     2761 cgccgggatt caggaccagc tcggcctcga actcccgtgc ggcctcgtcg agcttccccg
     2821 tcttcacata gatcaggccg gcgtaaaagt gcgcgaggag cactttgtcg tcgagcgcga
     2881 gcgcggtgcg taattcgccg agggccttgg cggcgtcgtt ctgctcgtag taggcccggc
     2941 cgagcaggat gtgaacctgc ggactttggc cgcccatcgc caccatctgc cgggtgacgc
     3001 gctcggcctc ctgcgtcttc ccgaccttgc cgagcgcgag cgcgagcggg tagtagaggg
     3061 ccgcctcgtc gggccgggcg gccacgactt cggccagcag cgccgaggct ccggcgtagt
     3121 tctccaacat gaaataactc agccccaaca aacgcttggc ctgaaggttg gacggccgag
     3181 ccttgatctc gttttcgagc ggcgcgacgg cgtctttgaa cgactcagac ttgaagtaag
     3241 caaggcccag attgtaatca agcccctcat gctgaggatt caatctcgcg gcggagctga
     3301 attgagcggc cgccgcgcga aagtcctgtc gctcggcgcg cagcaggccg atgttattgt
     3361 gggccgcggc aatcaccttc tcgtaaaacg acgcgtctgc cgccaacact tcccgctcgc
     3421 gcgcgtcggg cgcgacggag ccggcgacca tgcccggcgc cgagaccaac tccgggaact
     3481 tactctgctc gtccgcctcg gcgtcgttga ggaaggcgtc caacttcgcc ccgtcccgct
     3541 tgaaggcttt cgacttgagg tcggccgccg tctgcaactc gcgctcgccc tcctcggtgc
     3601 ggccggcctt cagcaacgtt tgcccgagcc ggtagtgggc gttcgtcacc tgataatcgt
     3661 tgtgccccaa tgatgggttg agcgcgatgg ctttcctgaa cgccgcaatc gcctgttcgt
     3721 acttccccaa attctggtaa gcctgcccga ggaagaagtg tgggtcgggg ttacggggct
     3781 ggatgcggga agccttctca aagagtggga cggccggcgg ccacttcccc tcgctggtgt
     3841 ggatgatgcc gaggtagtag ttggccaaaa actcgtcggg gtgggcggcc aactccgcct
     3901 gcaactcctt tgtcgcatcg ccgagacgcg ccgcgccgtc cttgagcaga taggtgcgtc
     3961 ccagatggta gtggacgcgc gggaagcgcg ggtcgagggc gacggccctt ttgaactcct
     4021 cgatggcctc ggcgagaaac cccgtctccc ggtaagcccg gccgatgagc acgcggagct
     4081 gtgggcggtc gccgagttgt gcgaccatgc gttcgtaaac ccgtctggca ccggcgaact
     4141 ggcgctgctt gaggtaggcc agaccgagcg tgtaggccgc gtcgtaatca tccggcttca
     4201 gcgccaatgc cgcttcgagt tcgcgcgcgg cgcccgcaaa ctcgcccatc ataaagtgag
     4261 tcttcccgag catgtggcgc gcgggggcgc tacgcgggtc gtgtgcgagg acgcggttca
     4321 aaggctcgac ggccttctgg tactgcccgg cgtaaaagta ggcgacggcc agcccgacga
     4381 gcacttcggg agagtcgggc cggtacggga cggccgcctc aagggccacg accgcctcgc
     4441 ggtagtttga ctgtgcggtg taaatcctcc cgagcgcggg gtacgcgccg gcgaggatgg
     4501 ccgcgtactc ggcctcggcg gcggccgtat tgccggcgcg ccggtgcgcc tcggccgcct
     4561 cgtgatgttt tctgaatgaa tcgctcgggg cttggaagtg tgggagcagg agcagaagcg
     4621 gcaggagggc cgaggcgaaa tacattgctt ccttgactgc ttcggtggac tcggcaaa
//
LOCUS       DASVES010000040         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_152155, whole genome shotgun sequence.
ACCESSION   DASVES010000040 DASVES010000000
VERSION     DASVES010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152155"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1059)
                     /locus_tag="VF240_01640"
     CDS             complement(67..1059)
                     /locus_tag="VF240_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX7173973.1"
                     /translation="MRADRLVSILLLLQMNGRMTARELAERLEVSERTIHRDMESLGA
                     AGVPVMAERGAGGGWMLMDGYRTNLTGLNDAEVQALFLGGPASVLADLKLDKASDAAL
                     IKLLASLPLSTRRDAEYARQRIHVDVSGWSRQEETVPCLHALQEAVWLNRRVRLGYRR
                     GVGAAEGETVERTVDPLGLVAKGSVWYLVGSVEGEPRSYRVSRVASVELLDDSCARPE
                     GFDLAAFWGRSNKEFKDKFPRFDAVFRARADVAPRLYFAGRFARVEGVGEPDAEGWVE
                     VRMRFQFDWEACEFALSFGPAVELVEPEELRGRVVRLAESVVEFYKRRAQARGN"
     gene            1192..2178
                     /locus_tag="VF240_01645"
     CDS             1192..2178
                     /locus_tag="VF240_01645"
                     /inference="COORDINATES: protein motif:HMM:NF006159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7173974.1"
                     /translation="MKSLNGKVAVVAGATRGAGRGIARGLAEAGAVVYCTGRSVRGNP
                     SPYGRPETVEETAEMINAAGGRGVHVRVDHTKEDEVRALFERVEREHGRLDVLANCVA
                     GEDMRMAGWTSFWQTDLKDGLAVFENALLTHVITAKHAAPLMIKKKRGLIVEVTEFDL
                     LFCGGNILAQLVKFSIKGLASMMAEELRKHRVAAVAITPGYLRSERMLEHFGVTEENW
                     REGGKKDPTFLQSESPLFIGRSVAALASDPKVMKKTGYLTSSYELGREYGLTDYDGRR
                     PDLGQHFVEHIIPSMKWMREGTERHVAWLDRIAGRGRYYLGEKQETADGRAA"
BASE COUNT          421 a          780 c          749 g          350 t
ORIGIN      
        1 gcttcctctg cgtcctctgc gcctctgcgg tgaacggccg tacctgaagt tcaactaaga
       61 gccaaactaa ttcccacgtg cctgcgctcg ccgcttgtag aattcgacga cgctctcggc
      121 gagacgcacc acacgcccgc gcaactcttc cggctccacc aactcgaccg ccggcccgaa
      181 gctcagcgcg aactcgcatg cctcccagtc gaactgaaag cgcatcctga cctcgaccca
      241 cccttcggcg tctggctcgc ccacgccctc gacgcgtgcg aagcgtcccg cgaagtagag
      301 gcgcggcgcc acgtcggcgc gcgctcggaa gacggcgtcg aagcgcggga acttgtcctt
      361 gaattctttg ttggagcgtc cccagaacgc cgcgaggtcg aacccctcgg ggcgcgcgca
      421 actgtcgtcc agcaactcga ccgaggcgac gcgcgagaca cggtagctgc gcggctcgcc
      481 ctccacgctc cccaccagat accagacgct ccccttcgcc accagcccga gcggatcgac
      541 cgtgcgctcg accgtctcac cctcggcggc gccgacgccg cgccggtagc cgaggcgcac
      601 gcggcggttc agccacacgg cctcctgaag cgcgtgcagg cagggcacgg tctcctcctg
      661 tcggctccat cccgacacgt caacgtgtat gcgctggcgc gcgtactccg cgtcgcggcg
      721 cgtggacagg ggcagcgacg ccagcagctt gatgagcgcc gcgtccgaag ccttgtcgag
      781 cttgaggtcg gccagcacgc tcgccgggcc gccgaggaaa agcgcctgca cttccgcgtc
      841 gtttaagccc gtcaggttcg tgcgataccc gtccatcagc atccacccgc cgcccgcccc
      901 gcgctcggcc atgacgggca cgccggccgc ccccagcgac tccatgtcgc ggtgaatcgt
      961 ccgctccgag acctccagac gctcggccaa ctcgcgcgcc gtcatgcgcc cgttcatctg
     1021 tagcagcaaa agaatcgaga cgagtctgtc agcccgcata agccctcctt tggcggcgtc
     1081 cgcagttagc cctgacttta ccaaacgaaa tatgacacaa ggtgtctgga atgaagagta
     1141 tttttcaaac ggaactcagg ggaagcgtcc gactacagga ggaggcaacc gatgaaaagt
     1201 ttgaatggaa aagtggccgt cgtcgccggg gcgacgcgcg gggctggccg agggatcgcg
     1261 cgggggctgg ccgaggcggg ggccgtcgtc tattgcacgg ggcggagcgt gcgcgggaac
     1321 ccgtcgccct acgggcggcc cgagaccgtc gaggagacgg ccgagatgat caacgcggcg
     1381 ggcggccgcg gcgtccacgt ccgcgtcgat cacactaaag aagatgaagt gcgcgcactc
     1441 ttcgagcgcg tcgagcgcga gcacgggcgt ctggacgtgc tggcgaactg cgtcgcgggc
     1501 gaagacatgc ggatggccgg gtggacttcc ttctggcaga ccgacctcaa ggacggcctt
     1561 gccgtcttcg agaacgccct gctcacgcac gtcatcacgg cgaagcacgc cgcgccgctg
     1621 atgatcaaga agaagcgcgg cctcatcgtc gaggtcaccg agttcgacct gctcttctgc
     1681 gggggcaaca tcctcgcgca gctcgtcaag ttttctatta aaggactggc gtcgatgatg
     1741 gccgaggagt tgcgaaagca ccgcgtggcg gccgtcgcca tcacgccggg ctacctgcgc
     1801 tcggagcgga tgctcgaaca cttcggcgtc accgaagaga actggcgcga gggggggaag
     1861 aaagacccga cctttctcca gtccgagtcg ccgctcttca tcggccgctc cgtcgccgcg
     1921 ctcgcctccg acccgaaggt catgaaaaag acgggctacc tcaccagctc ttacgagctg
     1981 gggcgcgagt atggcctgac cgactacgac ggccggcggc ccgacctcgg ccaacacttc
     2041 gtcgagcaca tcattccttc gatgaagtgg atgcgggagg gcacggagcg ccacgtcgcg
     2101 tggctcgacc gaatcgccgg gcgaggtcga tactatcttg gggagaagca ggagacggcc
     2161 gacgggcgcg cggcgtgatg cgcccgtgcg gagtgccggt caggtaagga ggctcggaag
     2221 ccccgaaggg gcgagatgtg atagcccggc gcagggcgct gggtagagag ccgcaagtaa
     2281 tcggggagta cagtaccgcg
//
LOCUS       DASVES010000041         9136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_152130, whole genome shotgun sequence.
ACCESSION   DASVES010000041 DASVES010000000
VERSION     DASVES010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152130"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1574
                     /locus_tag="VF240_01650"
     CDS             135..1574
                     /locus_tag="VF240_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7173975.1"
                     /translation="MKKPRLKNQLIVPALGAAFFGVVYYLLLNFAPAVPPESASLKTQ
                     KAFEVYGWLLNAAFVAMWLFLAFIIVRAAGAFVFYLVRKRKGQDAPSLVRDIFSLVVY
                     TVLVAAVLKYFYPDLSLTALLPTSALLGVIIGLALQDTLGNLFAGISLHADKPFQVGD
                     VIKVGQWTGVVESVTWRAVKIRTFQNHIVLFSNSSLGKESIEVCPRTNANARLVFFNA
                     VYSNSPVKVIHVVREAVREAENVLRYMTPIVRIRELGASGVEYEVKYWLQDYARYNDT
                     DALVRQRIWYAFRRAGLNFAYPTQTLLIQRQAGDGDGNFDGISERLSAVDIFAPLSAD
                     ELKALAAQTTRRVFAPGETILRAGDSGASMFVVHGGTVDVRVEQNGQPRTVNTLREGD
                     FFGEMALFTGEPRTADIIAVEETEVLEIGHAAMKRLFDTNPDLVESLSHVIADRRAGL
                     SARVEQAPDEQHESAGIIAAVRRFFGFDG"
     gene            complement(1658..2179)
                     /gene="bstA"
                     /locus_tag="VF240_01655"
     CDS             complement(1658..2179)
                     /gene="bstA"
                     /locus_tag="VF240_01655"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HEX7173976.1"
                     /translation="MDLRFPVGEFKPEESYSEEQQQLFLEQFEEAPAKLRRAVEGLSE
                     EQLDTPYRPGGWTVRQVAHHLPDSHMNGMIRMKLALTEDEPLIKTYMEDRWAELPDYK
                     TDPGVSLSLLDAVHQRWVAVWRSLGPAEFGRKFRHPDWGTLTLQQLLALYAWHGRHHV
                     GHITSLREREGWQ"
     gene            complement(2231..3229)
                     /locus_tag="VF240_01660"
     CDS             complement(2231..3229)
                     /locus_tag="VF240_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7173977.1"
                     /translation="MMANREKVAAYGAWAAVCFFWGTTYLAIRVGLETLTPMLFGGLR
                     FLIAGVVLFLIMSRQRNVRLPVGREWFDLCLVGLLLLGVGNGLVVWAEQWVSSGMAAL
                     LVATGPFWVAGLEASRRGGERVTLWSVAGMLVGFSGLAMLVAPNLFGASLGGKFLLGA
                     IGLQVACLSWAVGSVYSKQRPTGVNPLMGAAVQMLVAGAALTLVGSAAGEWGRIGFSA
                     RSLGALAYLVVFGSIVAYGSFTYAMQKLPLSIVSTYAYINPVIAIILGWMLLSEPLGW
                     RVWAATVVILGGVALVKAGPGLSARRAGRTTADDDERSDVAVSVAPRAAKACGASG"
     gene            complement(3226..3708)
                     /locus_tag="VF240_01665"
     CDS             complement(3226..3708)
                     /locus_tag="VF240_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7173978.1"
                     /translation="MIDEKDAKILEILQREGRTTNVELARAVELTPSSTLERVRKLEE
                     RGLIRGYTAILDPHALGLGLVAYIFVRVDDRDDVLGRSETTAEALAGLPSVQELHHLA
                     GEDCFLIKVRARDTDDLYRILRDEFGKFKTIRSTRTTIVLKTVKESAHLPVVRDVGGE
                     "
     gene            3980..4510
                     /locus_tag="VF240_01670"
     CDS             3980..4510
                     /locus_tag="VF240_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7173979.1"
                     /translation="MSITRYDPFRDLRSLQEEVNRLFSTNLTRSFADEGIARGAWMPS
                     VDIYENKDQIVLEAELPGMNREDFELTVENNVLTLRGERRFEKKDETDNYHRVERAYG
                     TFTRSFTLPQTVSGEGASAEYKNGVLRVVLKKREEVKARRIQIGGDDAGQTVARTIET
                     SAESPDSKNSKAAGGR"
     gene            4634..7234
                     /gene="clpB"
                     /locus_tag="VF240_01675"
     CDS             4634..7234
                     /gene="clpB"
                     /locus_tag="VF240_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX7173980.1"
                     /translation="MRLDRYTLRGQEAIQSAIEAAEKNQNQQVEPEHLLLAMLEQPEG
                     VVRPILGKLGANVQVVLNDLQAATGRLPKVQGGQQYFSPRLSQIFTASQKQAEKMQDE
                     YVSTEHLLLAIADEKDGAAGRILRQHGVNRDDLSKAIEQTRGGARITDQNAEANYQAL
                     SKYAKDLTELARQGKLDPVIGRDDEIRRTVQVLSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIISGDVPETLKNKRLVSLDLGSMLAGAKYRGEFEDRLKAVLKEIESSQGHIVLFI
                     DELHTLVGAGAAEGAIDASNMLKPALARGELRCVGATTLNEYKKHIEKDAALERRFQP
                     VVVGEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSNRYITDRFLPDKAIDL
                     IDEAASRLRIEIDSLPQEIDVLEREILQLEIERQALQREEDDRSKARLSDIEQRIADL
                     KEKSSGMKAKWQSEKEEIERMREAKNELEQLRLQLDQARNAGDLTRAAELQYGRIPEL
                     ERTFAEEREKLAELQRDGVMLKEEVDEEDVALVVAKWTGVPVTKMLEGEMQKLVSMEE
                     RLSKRVIGQDEALAAVANAVRRARAGLQDPNRPIGSFIFLGPTGVGKTETARALAEFM
                     FDDERAMIRLDMSEYMEKHAVARMIGAPPGYIGYEEGGQLTEAVRRRPYSVVLFDEVE
                     KAHQDVFNVLLQILDDGRLTDSKGRTVDFKNTVLIMTSNLGSREIQEADGDEEQVRDD
                     VLEILRGHFKPEFLNRVDDIVIFKQLSREQIADIIGIQLERLLKTLADRDISLVLDDS
                     AKALLAREGYDPLYGARPLKRAIQTLVQNPLASQLLRGEALPGQTIRVSAEGDRMKFT
                     TDAATTTAAG"
     gene            7375..7845
                     /locus_tag="VF240_01680"
     CDS             7375..7845
                     /locus_tag="VF240_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7173981.1"
                     /translation="MARHTWDPMRDLMTLQDRMNRLFEDTAERRAPRGGSASGEAEDE
                     MERADWSPAADVYEREAEYVIVLDLPGVEREGLDVGLDESRLTVRGERAPAETDAHVR
                     RAERPAGRFVRSFTLPETVERGQISADYKDGVLRLRLPKRGEREQPRRVEIKVE"
     gene            7960..8637
                     /locus_tag="VF240_01685"
     CDS             7960..8637
                     /locus_tag="VF240_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7173982.1"
                     /translation="MEETMGAEETRIEGRESGQEGERIRVTDRRRVRADEEGGAADAR
                     EDGPSGAPSLKPTYVEELETRTRAAEQLAREVQARFEQVKAELRRETDELRQRLNRNA
                     DERAGREKANFVAALLPVIDNLQRAVEAAEGGGSRESLLDGLRGTISGFEVALTQAGA
                     EPVEAVGQRFDPELHEAVDTTEVDAERDGMVTAQYGRGYRMGKQLLRPARVQVGRARG
                     EAQAATE"
     gene            8782..>9136
                     /locus_tag="VF240_01690"
     CDS             8782..>9136
                     /locus_tag="VF240_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX7173983.1"
                     /translation="MAAKDVKFREEARAAMLKGVNTLANAVRVTLGPKGRNVVLGKKF
                     GSPVITKDGVTVAKEIQLKDNYENMGAQMVREVASKTNDIAGDGTTTATVLAQAIFRE
                     GVKNVTAGANPMLLQR"
BASE COUNT         1784 a         3055 c         2823 g         1474 t
ORIGIN      
        1 cgcggtactg tataatgccg ccgcgccgcg cccaccgcat aacgtcatgc gccgcaaagc
       61 tcgtgaatcg taaatcggaa gagttatcaa cgattcacga ttcacatggt tgttgtatct
      121 gttgagttga ccgcgtgaag aaaccccgtc ttaaaaatca gttgatagtg cccgcgctgg
      181 gggcggcgtt cttcggcgtc gtctactacc tgctgctgaa cttcgcgccg gcggtcccgc
      241 ccgagtcggc ctcgttgaag acgcagaagg cgttcgaggt ctacggatgg ctgctcaacg
      301 ccgccttcgt cgcgatgtgg ctgttcctgg ccttcatcat cgtgcgggcg gcgggcgcct
      361 tcgtcttcta cctcgtccgc aagcgcaagg ggcaggacgc gccgagcctc gtgcgcgaca
      421 tcttctcgct cgtcgtctac accgtcctcg tcgccgccgt cctcaagtac ttctaccctg
      481 acctttcgct gacggcgctt ttgccgacct cggccctgct cggcgtcatc atcggcctcg
      541 cgcttcagga cacgctcggc aacctcttcg ccggcatctc gctccacgcc gacaagccgt
      601 ttcaggtggg cgacgtgatc aaggtcgggc agtggacggg cgtcgtcgag agcgtcacgt
      661 ggcgggccgt gaaaatccgc acgttccaga accacatcgt cctgttcagc aacagcagcc
      721 tcggcaagga atctatcgag gtctgcccgc gcacgaacgc caacgcgcgc ctcgtcttct
      781 tcaacgccgt ctactcgaac tcgcccgtca aagttatcca cgtcgtgcgc gaggccgtgc
      841 gcgaggccga aaacgtcctg cgctacatga cgccaatcgt ccgcatccgc gaactgggcg
      901 cgtcgggcgt cgagtacgag gtcaagtact ggctccagga ctacgcgcgc tacaacgaca
      961 cggacgcgct cgtccgccag cgcatctggt acgccttccg ccgcgccggc ctcaacttcg
     1021 cctacccgac gcagaccctc ctcatccagc gccaggcggg cgacggcgac ggcaacttcg
     1081 acggcatctc cgaacgcctc tcggccgtgg acatcttcgc gccgctctca gccgacgagc
     1141 tgaaggccct cgccgctcag acgacgcgac gcgtcttcgc ccccggcgag actatcttgc
     1201 gcgcgggcga tagcggggcg tcgatgttcg tcgtccacgg cgggacggtg gacgtgcgcg
     1261 tcgagcagaa cggccagccg cgcaccgtga acactctgcg cgagggcgac ttcttcggcg
     1321 agatggccct cttcaccggc gagccgcgca cggccgacat catcgccgtc gaggagaccg
     1381 aggtgctgga gatcggccac gccgcgatga agcgcctgtt cgacaccaac cccgacctcg
     1441 tcgaatcgct cagccacgtc atcgccgacc gccgcgccgg cctcagcgca cgcgtcgagc
     1501 aagcgcccga cgagcagcac gaatccgccg gcatcatcgc cgccgtccgc cgcttcttcg
     1561 gcttcgacgg atgaaaagcc gtaaatcgaa aatcgtcaat cgtaaatcga atcaagcagt
     1621 ttcgatttac gattgacgat tttcgattta cggcttttta ctgccagcct tcgcgctcgc
     1681 gtaaggatgt gatgtgtccg acgtggtggc ggccgtgcca ggcgtagagg gcgaggagct
     1741 gttggagcgt gagcgtcccc cagtcggggt gtcggaactt gcgcccgaac tccgcggggc
     1801 cgagcgacct ccagacggcg acccagcgtt gatgtacggc gtcgagaagc gagagagaca
     1861 cgccgggatc agtcttgtag tcgggcagct cggcccagcg gtcttccatg taagtcttaa
     1921 tcagcggctc gtcctcggtg agcgcgagct tcatgcgaat catgccgttc atgtgactgt
     1981 cgggcaggtg gtgggcgacc tgccgcaccg tccagccgcc cggtcggtag ggcgtgtcga
     2041 gctgctcctc cgacaacccc tcgaccgctc gcctcaactt cgccggcgcc tcctcaaact
     2101 gttcgaggaa gagttgctgc tgctcctccg aataactctc ctccggcttg aactctccga
     2161 cggggaaacg taagtccatc aggtgtgcct ccctttcatc tcttaaactc gaatgacact
     2221 cccccttgcc ctacccgctc gcgccgcacg ccttcgcggc gcgcggagct acggacaccg
     2281 caacgtctga acgctcgtcg tcgtcggccg tcgtcctccc tgcccttcgc gcggagagtc
     2341 ccggcccggc cttgacgagc gccacaccgc cgaggatgac gacggtcgcc gcccacacgc
     2401 gccaccccag cggctcactc agcagcatcc aaccgaggat gatagcgatc accgggttga
     2461 tgtaagcgta ggtcgagacg atggagagcg gcagcttctg catcgcgtag gtgaatgagc
     2521 cataggccac aatcgagccg aacacgacga ggtaggcgag cgcgcccaga ctgcgcgcgc
     2581 taaatccgat gcgcccccac tcgccggccg cgctccccac gagcgtcagc gccgcgcccg
     2641 ccacgagcat ctgcaccgcc gcgcccatca aagggttcac acccgtcggc cgctgcttgg
     2701 agtagaccga ccccaccgcc cacgacagac acgccacctg tagcccgatg gcgcccagta
     2761 gaaacttgcc gcccagagac gcgccgaaaa gattaggcgc gaccagcatc gccagccccg
     2821 agaagccgac gagcatcccg gccacagacc agagcgtgac gcgctcgccc ccacgccgcg
     2881 acgcctccag acccgccacc cagaaaggcc ccgtcgccac cagcagcgcc gccatcccac
     2941 tcgaaaccca ctgctcggcc cacaccacca gcccgttgcc gacgcccaga agcaacagcc
     3001 ccaccagaca caaatcgaac cattccctcc ccacgggcag gcgcacgttc ctctgccggc
     3061 tcattatgag aaagagcacc acgccggcga tcaggaaccg cagacccccg aagagcatcg
     3121 gcgtcagtgt ctccagcccg acgcgaatcg ccagataggt cgtgccccag aaaaagcaga
     3181 ccgccgccca cgccccgtaa gccgccacct tctccctgtt cgccatcatt cacccccgac
     3241 atccctgacc accggcagat gcgccgactc cttgaccgtc ttcagcacaa tcgtcgtccg
     3301 cgtgctccga atcgtcttga atttgccgaa ctcgtcccga agaatcctgt agaggtcgtc
     3361 cgtgtcgcgc gcgcgcacct tgataaggaa acagtcctcg cccgcgaggt ggtgcagctc
     3421 ctgcacggag gggagaccgg caagcgcctc ggccgtcgtc tccgagcggc ccagcacgtc
     3481 gtcgcggtcg tccacgcgca cgaagatgta ggccacgagg ccgaggccga gggcgtgcgg
     3541 gtcgaggatg gccgtgtagc ccctgatcag cccgcgctct tcaagcttgc gaacacgctc
     3601 gagcgtcgat gaaggggtca gctcgacggc gcgcgccagt tccacattcg tcgtccgccc
     3661 ctctctctga agaatctcaa gaattttggc gtctttctcg tcaatcatgc cgctttgtgc
     3721 ccccatctga ctaattgcac gaacattgta accctgatac cgctttatgc caactattat
     3781 tttgatatat cccaccatcg ccgaatcact gccaatcggc taaccatcgt ccggcgggac
     3841 aagcctactt gacaaggcgg cactcaacct ttattatcca cgtgcgctca ccagccgaca
     3901 acttaccact tctattcgct cggcgggtgg gctttccaat accgtcgatc acttttagag
     3961 gctttaggag gtttgactta tgagcattac acgttacgac ccgttccgcg acctgcgtag
     4021 cctacaggag gaggtcaacc ggctcttctc caccaatctt acccgctcgt tcgccgacga
     4081 gggcatcgcc cgcggcgcct ggatgccgag cgtggacatc tacgaaaaca aggatcagat
     4141 cgtgcttgag gctgaactgc ccggcatgaa ccgtgaagac ttcgagctga cggtcgagaa
     4201 caacgtgctc actctgcgcg gagagcgccg cttcgagaag aaggacgaga cggacaacta
     4261 ccaccgcgtc gagcgcgctt acggcacctt cacgcgctct ttcacgctgc cccagaccgt
     4321 ctcgggcgag ggcgcgtcgg ccgagtacaa aaacggcgtg ctgcgcgtcg tgctcaagaa
     4381 gcgcgaggag gtcaaggccc gtcgcattca aatcggcggc gacgacgccg gacagaccgt
     4441 ggcgcgcacc atcgagacga gcgccgagtc gcccgactcg aagaacagta aggcggccgg
     4501 cggccgttaa ttaggaaagc tgttagctgt tagcggtcag caatcagcat gagcaagcga
     4561 tggacacggg gcgtgtcttt agctgagtgc taaccgctaa cagctaacag ctttaagacg
     4621 acggacaatt tttatgcgtt tggatcgcta cacgctgcgc ggccaggagg ccatccagtc
     4681 ggccatcgag gcggcggaga agaatcagaa tcagcaggtc gagccggagc accttctgct
     4741 cgccatgctc gaacagccag agggcgtggt gcgtcccatc ctcggcaagc tcggcgccaa
     4801 cgtgcaggtc gtcttaaacg acttgcaggc ggccacgggg cgcctgccca aagtgcaggg
     4861 cgggcagcag tacttcagcc cgcgtctcag ccaaatcttc acggcctcgc agaagcaggc
     4921 cgagaagatg caggacgagt acgtctcgac cgagcacttg ctcctcgcca tcgccgacga
     4981 gaaggacggc gccgcgggcc gcatcctgcg ccagcacggc gtcaaccgcg acgacctctc
     5041 gaaagccatc gagcagacgc gcggcggcgc gcgtatcacc gaccagaacg ccgaggccaa
     5101 ctatcaggct ctctcgaagt acgccaaaga cctgacggag cttgcgcgtc agggcaagct
     5161 cgaccccgtc atcggccgcg acgacgagat acgccgcacc gttcaggtgc tctcgcgccg
     5221 caccaaaaac aatcccgtcc tcatcggcga gccgggcgtc ggcaagacgg ccatcgtcga
     5281 gggactcgcg cagcgcatca tctccggcga cgtgcccgag acgctcaaga acaagcgtct
     5341 cgtgtcgctc gacctcggct cgatgctcgc gggcgccaag tatcgcggcg agttcgagga
     5401 tcgtttgaag gccgtcctca aggagatcga aagctcgcag gggcacatcg tcctcttcat
     5461 cgacgagctg cacacgctcg tcggcgccgg cgcagccgag ggcgccatcg acgcctcgaa
     5521 catgctgaag cccgctctcg cccgcggcga gctgcgctgc gtcggcgcga ccaccctcaa
     5581 cgagtacaaa aagcacatcg agaaagacgc cgcccttgag cgtcgcttcc agcccgtcgt
     5641 cgtcggcgag ccgaccgtgg aagacaccat cgccatcctg cgtggcctga aggaacgtta
     5701 cgaggtgcat cacggcgtcc gcatcaagga ctcggccatc gtcgccgccg ccacgctctc
     5761 gaaccgttac atcaccgacc gcttcctccc cgacaaggcc atcgacctca tcgacgaggc
     5821 cgcctcgcgt ctccgcatcg agatcgattc cctgccgcag gagatcgacg tgctggagcg
     5881 cgaaatcctg caactcgaaa tcgagcgtca ggctctccag cgcgaggagg acgaccgctc
     5941 gaaggcgcgt ctgagcgaca tcgagcagcg catcgccgac ctcaaggaga agtcttcggg
     6001 gatgaaggcc aagtggcaat ccgagaagga agagatcgag cggatgcgtg aggctaaaaa
     6061 cgagctggag cagttgcgcc tgcaactgga tcaggcgcgc aacgccggcg acctcacacg
     6121 cgccgccgaa ctccagtacg gcaggattcc cgagcttgag cgcaccttcg ccgaggagcg
     6181 cgagaagctg gccgaattgc agcgcgatgg cgtgatgctc aaggaggagg ttgacgagga
     6241 ggacgtggcg ctggtcgtcg ccaagtggac gggcgtgccc gtgacgaaga tgctcgaagg
     6301 cgagatgcag aagctcgtct cgatggagga acgtctctcc aagcgcgtca tcgggcagga
     6361 cgaggcgctt gcggccgtgg ccaacgcggt gcgccgcgcc cgcgccggtc tgcaagaccc
     6421 gaatcggcct atcggctcat tcatcttcct cggccccacc ggcgtcggca agaccgagac
     6481 ggcgcgggcg ctcgccgagt tcatgttcga cgacgagcgc gcgatgattc gcctcgacat
     6541 gtcggagtac atggagaagc acgccgtcgc ccgcatgatc ggcgcgcccc ccggctacat
     6601 cggctacgag gaaggcgggc aacttaccga ggccgtgcgt cgtcgtccgt actcggtcgt
     6661 cctcttcgac gaggtcgaga aggcgcacca ggacgtattc aatgtcctct tgcagatact
     6721 cgacgacggc cgcctcacgg actcgaaggg ccgcaccgtt gacttcaaga acacggtgct
     6781 gattatgact tcgaacctcg gcagccgcga gattcaggag gccgacggcg acgaggagca
     6841 ggtgcgcgac gacgttctgg aaatcctgcg cggccacttc aagccggagt tcctgaatcg
     6901 cgtggacgac atcgtcatct tcaagcagct ctcgcgtgag cagatcgccg acatcatcgg
     6961 catccagctc gaacgcctgc tcaagacgct cgccgaccgc gacatctcgc tcgtcctcga
     7021 cgactccgca aaagcattgc tcgcgcgcga gggctacgat ccgctctacg gcgcgcgccc
     7081 cctcaagcgc gctatccaga cgcttgtcca gaacccgctc gcgtcgcagc ttctgcgcgg
     7141 cgaggctctg cccggccaga ccatccgcgt ctcggccgag ggcgaccgca tgaagtttac
     7201 gaccgatgcc gcgacgacga ctgcggccgg gtaaaaagat gccggcgcgc gcagtaccgc
     7261 gagctgtagc gagcgggtgc gcccgcgact accaagcccg ctcggcgcgc gcgagacccg
     7321 ctcgccaccg ctcgcggtac tgtgcttgag ttaatccttt taggagttga acgaatggcg
     7381 aggcacacct gggacccgat gcgcgacctg atgacgttgc aggatcgcat gaaccgtctc
     7441 ttcgaggaca cggcggagcg gcgcgcgcct cgcggcggca gcgctagcgg cgaggcggag
     7501 gacgagatgg agcgggccga ctggtctccg gccgccgatg tttacgagcg cgaggcggaa
     7561 tacgtcatcg tgctcgatct gcccggcgtc gagcgcgaag gattggacgt gggtctcgac
     7621 gagagccgac tgacggtgcg cggcgagcgc gcgccggccg agacggacgc gcacgtgcgc
     7681 cgcgccgagc gccccgcggg ccgcttcgtt cgcagcttca cactccccga gacggtcgag
     7741 cgcggccaga tcagcgccga ctacaaggac ggcgtgttgc gcctgcgcct gcccaagcgt
     7801 ggtgagcgcg agcagccgcg ccgtgtggaa atcaaagtgg agtgatgagt gacaagtgat
     7861 aagtgataag atggttttgt tttttctcat cacttgtcac tcatcactta tcactggtgt
     7921 catatggtat cttttcgcaa gaacaggaat aagaacgaag tggaagagac gatgggggcc
     7981 gaagagacgc gcatcgaggg acgcgagtcc gggcaggagg gcgagcgcat ccgcgtgacc
     8041 gaccgccggc gcgtccgcgc agacgaggag ggcggcgcgg cggacgcgcg cgaagacggc
     8101 ccgtcgggcg cgcccagtct gaagccgact tacgtcgagg agttggagac gcgcacacgc
     8161 gcggccgagc aactggcccg cgaggtgcag gcgcgcttcg agcaggtgaa ggccgaactc
     8221 cgacgcgaga ccgacgagct gcgccagcgt ctcaatcgca acgccgacga gcgcgccggg
     8281 cgcgagaagg cgaacttcgt cgcggcgctg ctgccggtca tcgacaatct ccagcgcgcc
     8341 gtcgaggccg ccgagggcgg cggctcgagg gagtcgctgc tcgacggcct gcgcggcacg
     8401 atcagcggct tcgaggtcgc actcacacag gccggcgccg agccggtcga ggccgtgggc
     8461 cagcgtttcg acccggagtt acacgaggcc gtggacacca cggaggtgga tgcggaacgc
     8521 gacggcatgg tgacggcgca gtacggccgc ggctaccgca tgggcaaaca gctcctgcgg
     8581 cccgcgcgcg tgcaggtcgg ccgcgcgcgc ggcgaggcgc aggcggcgac agagtaaaga
     8641 agctgttagc tgttagctat tagctattag ctgtcagccc acggcagccg ccgtgttata
     8701 ggttgagcgt cgcagctcac gctctgttta ttcgctttct taaaagctaa cagctattca
     8761 aaacgcagag gagaaaccta agtggcagcg aaggatgtga agttcaggga agaagcgcgg
     8821 gcggcgatgt tgaagggtgt gaacacgctg gcgaacgccg tgcgcgtgac gctcgggcct
     8881 aaaggtcgga acgtcgtgct cggcaagaag ttcggctcgc ccgttattac caaggacggc
     8941 gtgacggtcg ccaaggagat tcagctcaag gacaattacg agaacatggg cgcgcagatg
     9001 gtgcgcgagg tcgcgtcgaa gacgaacgac atcgcgggcg acggcaccac gacggccact
     9061 gtgctcgcgc aggccatctt ccgcgagggc gtcaagaacg tcacggccgg cgccaacccg
     9121 atgttgctcc agcgcg
//
LOCUS       DASVES010000042         5550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_734834, whole genome shotgun sequence.
ACCESSION   DASVES010000042 DASVES010000000
VERSION     DASVES010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734834"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VF240_01695"
     CDS             <1..224
                     /locus_tag="VF240_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173984.1"
                     /translation="GWRVPPYNETQNYVRRIFARYSLIRDPQSARLAPQVTAQQVATI
                     KEMEKPGVIYSKSVFVVRLPDGKLRLVSQ"
     gene            complement(563..3208)
                     /locus_tag="VF240_01700"
     CDS             complement(563..3208)
                     /locus_tag="VF240_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7173985.1"
                     /translation="MKAERWQEVERLYHAALERGAEGRAAFLAEASAGDEGLRREVES
                     LLAYEDRAENFIESPALEVAAKMMAEGQGAPVLAGQTINHYRVISPLGAGGMGEVYLA
                     SDTRLGRRVALKFLPAHFSQDKEHLRRFEQEARAVAALSHPNVCTIYEVVETGEGRHC
                     IVMEHVGGVTLRTRMRGRRMSLAAAVDIALQIASALAAAHHAGIVHRDIKPENVMVRP
                     DGLVKVLDFGIAKYAGPARGRDAKESWVKTATGVVVGTTAYMSPEQARGQAVDVRSDI
                     WSLGVILYEMVARRLPFPGKTPSDRVAAILEREPEPLGRARRGVPAELERVVSRALAK
                     DRDERYAGAADLTKDLRELRGRLGAEPHFRFALPAPARRLLFSRKRRGVALAALLLVI
                     AAIVAGPAYLFFRRSSTPAPQTEIKLLAVLPLKSLNRAAGDDYLGLGIADTIITKVSR
                     LGGLTVRPTSAVRKYADQEIDSLEAAGQLQADAVLDGTFLRAGDRLRVSVNLLRARDG
                     ASLWAESFDMRFTDIFAIQDEVSKQVAAQLQLRLSAAEQARLAKRHTTSPEAYSYYTK
                     AMYHFSKRGFSGDPQEETDTAIDLFKKAIELDPNYALAHAQLGFAYAWLADFREGGMG
                     LIASAKEALRVAERLDPQLAEVHVARAFIFWSHYEGWQNEAAMRELNMAKQLDPSTSN
                     NVLPALYYHIGLEEQSVSEYELALERDPTSDTIKNSYGFLYLHVARPDDWLALNQRLF
                     NRGPDVRYYLEKRMLKEAAPLVEETYVKNPDAPESRRNKALLLALQGKHREAQAAIPR
                     ILEKAPRDKGYHHYTYNNARIYALDGKSEEALQWLRITVKEGFPCYTLFARDPFLDPI
                     RKDSAFIQFMSEMEARWEGYRREFG"
     gene            complement(3198..3788)
                     /locus_tag="VF240_01705"
     CDS             complement(3198..3788)
                     /locus_tag="VF240_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7173986.1"
                     /translation="MSRPTPNEITGLLVAWGGGDESALERLMPLVYDELRRLARRYMS
                     HERPGHTLQTTALVNEAYLRLVNWQEVRWQNRAHFFAVSAQMMRRILVDFARDRQYLK
                     RGGGALRVSLAEAASFSVGRDEDLVALDEALTALAEMDRRKARVVEMRFFGGLSVREV
                     AEVLNVSEETVMRDWRLAKVWLLRELGREGAAGGES"
     gene            4237..5409
                     /locus_tag="VF240_01710"
     CDS             4237..5409
                     /locus_tag="VF240_01710"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX7173987.1"
                     /translation="MTDWNAIAVQATITGGRPGPTGVLDIAMVQAAVYDAVQAIERRY
                     EPYYVEIPGASGSSVAAAAKAAHDVLVHLFPAPTQVAQLDTAYQQYLLLHGLSETDPG
                     VAVGATAAAGIIALRAGDGSFPTPAPTPFTGGAEPGVWRPTPPNNLPMAVSWLGAVTP
                     FTLKEPSQFRAAPPPPLNSGRYARDYDEVKAIGARTGSSRTPEQTDIAHFWAGNTPVI
                     WNRALRDIAEAHVDNIADSSRLFALTSMAIADALITAWNDKLHYAFWRPITAIREGDH
                     DGNDRTHGDPLWESLITTPNYPDYTSGAVNFATATTHVLAFFFGTDDFTFSVTTTNTV
                     PTVADTRTYERFSDVAEELVEARIYSGIHFRFADEAAHKQGKQVAMWAFNHYMRPR"
BASE COUNT         1010 a         1881 c         1636 g         1023 t
ORIGIN      
        1 acggctggag ggttccgccc tacaacgaga cgcagaacta cgtccgacgc atcttcgccc
       61 gctactcgct catccgcgac ccgcagtcgg cacgcctcgc gccgcaggtg acggcgcaac
      121 aggtcgcgac catcaaggag atggagaagc cgggagtgat ctactcgaag agcgtcttcg
      181 tcgtgcgcct gcccgacggc aagctgcgtc tggtcagtca gtagatgcgg tcggcaacgg
      241 ccccgcccga aacgacgaaa gggacgagac ccgagtggtc ttcgtgaggg tgcgcgccgc
      301 gaggccgaag aaatcggcgc gcgcgccgtc gtcactttgc cttataattc cgaagagctt
      361 ttggagtcga tcaaagcgtc cgggagcgcc gagtgaggcg gggaaagaga tgttttactc
      421 gccgcacacc gccgcagttg cctgttggtt gctccgattc agacatgccg cggcgagtta
      481 agttgacaat accgtcggac ttcatctcgc tcgttgccat tgtagtccgc gccggaatgt
      541 gttgacgttg gcgtagagcc gctcacccga attcacgccg gtagccctcc caacgggcct
      601 ccatctcgct catgaattga atgaaggccg aatcttttct gatcggatcg agaaatgggt
      661 cacgcgcaaa gagcgtgtag catgggaatc cctctttcac cgtgatgcgc agccactgca
      721 aggcttcctc gcttttacca tccagcgcgt agatgcgggc gttattatac gtgtagtgat
      781 gatacccctt gtcccttgga gctttctcca atattcgggg aattgccgcc tgagcttccc
      841 tgtgcttacc ctgtaaagcc agcaacaagg ctttgttacg gcgtgattcc ggcgcgtcag
      901 ggttcttaac atatgtctcc tcgacgagcg gcgcggcctc cttcagcatc ctcttttcca
      961 gatagtagcg aacgtccggc ccgcgattga acaacctctg attcaatgcc agccagtcat
     1021 ccggtctggc tacgtgcaaa tacaggaagc cataagagtt tttaatggtg tcgctcgtag
     1081 ggtctcgttc cagcgctaac tcatactcac taaccgactg ctcttcaaga ccgatatgat
     1141 aatagagcgc tggcagtacg ttattggaag tgctcggatc aagctgcttc gccatgttta
     1201 gctcgcgcat cgccgcttca ttctgccaac cttcgtaatg gctccagaag ataaaagcac
     1261 gcgcgacatg gacttcggcc agttgtgggt cgagccgctc cgccacccga agcgcttctt
     1321 tggcgctcgc tatcaggccc atcccccctt ccctgaagtc tgctagccag gcataagcga
     1381 atccgagctg ggcatgagcc agagcatagt tcgggtcaag ctcaatcgct ttcttaaaca
     1441 gatcaatcgc cgtatctgtt tcttcttgtg ggtcgccgga aaagccgcgt ttgctgaagt
     1501 gatacatcgc cttcgtatag taactatagg cttcagggct tgtcgtgtgg cgcttggcca
     1561 gccgcgcctg ttccgccgcg ctcagcctga gctgcaactg cgccgccacc tgcttcgaca
     1621 cttcgtcctg aatcgcgaag atgtcggtga agcgcatgtc gaaactctcg gcccagagcg
     1681 atgcgccgtc gcgcgcccgc agaaggttga cgctcacccg cagccggtcg cccgcgcgca
     1741 ggaatgtgcc gtcgagcacc gcgtcggcct gtaactgccc ggcagcttcc agcgaatcga
     1801 tctcctgatc ggcgtacttg cgcacggcgc tcgtcggacg cacggtcagt ccgccgagtc
     1861 ggctgacctt cgtgatgatc gtgtcggcaa ttcctaaccc gaggtaatcg tcgcccgccg
     1921 cacggttaag cgatttcagc ggcaacacgg cgagcaactt gatttcggtt tgcggtgctg
     1981 gcgtcgagct tcggcgaaag aacaggtaag caggcccggc aacgatggcg gcgatgacga
     2041 gcaggagcgc cgccagcgca accccccggc gcttgcggga gaagagtaga cggcgcgccg
     2101 gcgctggtaa ggcgaagcgg aagtgaggct cggcgccgag cctcccccga agctcgcgta
     2161 aatccttagt caggtcggcg gccccggcgt agcgttcgtc ccggtctttg gccagcgcgc
     2221 ggctaacgac ccgttccagc tcggcgggga cgccgcgccg cgcacggcca agcggctccg
     2281 gctcgcgctc caggatggcg gccacgcgat ccgaaggcgt cttgccgggg aacggcaggc
     2341 gccgggcgac catctcatag aggatcacgc ccagagacca gatgtccgag cgcacgtcca
     2401 cggcctgccc gcgcgcctgt tcgggcgaca tataggctgt tgtcccgacg acgacgcccg
     2461 tcgccgtctt gacccacgac tctttcgcgt cgcgcccgcg ggccggcccg gcatacttgg
     2521 cgatgccgaa gtcgaggact ttcaccagcc cgtccggcct gaccatgacg ttctccggct
     2581 tgatgtcgcg atgcacgatt ccggcgtggt gcgccgccgc cagtgcgctc gcgatttgca
     2641 gcgcgatgtc tacggcggcg gcgagcgaca tcctcctgcc cctcatgcgc gtgcgtagcg
     2701 tcaccccccc gacgtgctcc atcacaatac agtggcggcc ttccccggtc tcgaccacct
     2761 catagatcgt gcagacgttc gggtgcgaca gggccgcgac ggcgcgggcc tcctgctcga
     2821 aacggcgcag gtgttccttg tcttgactga aatgcgcggg cagaaacttg agcgcgactc
     2881 tgcgccccag acgggtgtcg gaggccaggt agacttcgcc catcccgccc gcgccgagag
     2941 gggagatgac acggtagtga ttgatcgtct gccccgccaa caccggcgcg ccctgcccct
     3001 cggccatcat cttcgccgcg acctccagag ccggcgactc gatgaagttt tcagcccggt
     3061 cttcatacgc gagcagcgac tccacttcgc ggcgcagccc ctcgtcgccc gccgaggctt
     3121 cggcgaggaa ggcggcgcgc ccctccgcgc cgcgctccag cgccgcgtgg tagagtcgct
     3181 cgacctcctg ccaacgctca gctttcaccg cccgccgccc cctcccgccc aagctccctc
     3241 aggagccaca ccttcgccag ccgccagtcc ctcatcacgg tctcctccga gacattcagc
     3301 acctcggcca cttccctgac cgacagcccc ccgaagaaac gcatctccac cacccgggcc
     3361 ttccgccgat ccatctcggc cagcgccgtg agcgcctcgt cgagcgcgac caaatcctcg
     3421 tcgcgcccca cgctgaatga tgccgcctcg gcgagcgaga cccgcagcgc gccgccgccg
     3481 cgcttgaggt actgcctgtc gcgggcgaag tccaccagga tgcggcgcat catctgcgcc
     3541 gagacggcaa agaagtgcgc tcggttctgc caacggactt cttgccagtt gactagccgc
     3601 aaataagctt cattgacgag cgccgtggtt tggagcgtgt ggccggggcg ctcatggctc
     3661 atatagcggc gggccagccg gcgcaactca tcgtaaacga ggggcatcag ccgttcgagc
     3721 gccgactcgt caccgccgcc ccacgccacg agcagcccgg ttatttcgtt tggtgtgggt
     3781 cgcgacatcc gaaccctacc ttccggcgcg gaactaaacc gcaggcgcgg ctgaccgcgg
     3841 cgcccggccc tagtcctcca cagggaaaga agaccgagta aattttttta gaggctttgc
     3901 agttcttcgc ctcatttccc gccttagtaa atgagggggc agggtcgccc agtgctctta
     3961 aagactcttt agtcgggggc cggtgatttt gtgcgtgtgg ccacggcaac tctgcgctcc
     4021 ggccaattgc acctgaggga caagaccatt ttaggtgcgg cgcgcggcag gcgcaatccg
     4081 gtgaagttca tagatgaatc gccttcgaca caatgtcgat aaacatcaat ccactaacga
     4141 aaggaacatc tcatgaagct gaaacttcaa accttacgaa gcatcgccat catcgcctta
     4201 ttggttgcgg cccctgccag cgtcagcgcc gatgtcgtga ccgactggaa cgccatcgcg
     4261 gtgcaggcga ccatcaccgg aggacgtccg ggtccgaccg gcgtcctcga cattgccatg
     4321 gttcaggccg ccgtttatga cgccgtccaa gccatcgaga ggcggtacga accctactac
     4381 gtggagattc ccggagcctc cggctcctcg gtggcggcgg ccgccaaggc cgcgcacgac
     4441 gtgctggtcc acctctttcc cgctcccacg caggtcgcgc agctcgacac ggcctaccaa
     4501 cagtatttgt tgctacacgg gctgtcagag accgatccgg gtgtagcggt gggcgcgacg
     4561 gccgccgccg gcatcatcgc cctgcgggct ggtgacggga gcttcccgac ccccgcgccg
     4621 acgcccttca cggggggcgc cgaacccggc gtctggcgcc ctactccgcc gaacaacctg
     4681 ccgatggccg tgtcgtggct gggcgccgtc acccccttca cattgaaaga gccttcacag
     4741 tttcgtgccg cgccgccgcc gcctctgaac agcggccggt acgcgagaga ctatgacgag
     4801 gttaaagcca taggagcacg taccggcagc tcacgcaccc ccgagcagac cgacatcgcc
     4861 cacttctggg ccggtaacac ccccgtcatc tggaaccgcg ccctgaggga catcgccgag
     4921 gcgcacgttg acaacatcgc tgatagttcc cggctcttcg ccctgactag catggcgatt
     4981 gccgacgcgc tcatcaccgc gtggaacgac aagctccact acgccttctg gcggccgatt
     5041 accgccatcc gcgaggggga ccacgacggc aacgaccgga cgcacggcga ccctctctgg
     5101 gagtctctca ttaccacccc gaactatcct gactacacct cgggcgcagt caatttcgcc
     5161 accgccacga cccacgtgct ggcgttcttc ttcgggacgg acgacttcac cttttcggtg
     5221 acgaccacca acacagtccc gaccgtcgcg gacacacgca cgtatgagcg cttctcggat
     5281 gtcgccgagg agcttgtcga ggcccgtatc tattcgggca tccacttccg cttcgccgac
     5341 gaagcggccc acaagcaggg aaaacaagtg gccatgtggg ccttcaatca ctacatgcgg
     5401 ccccgctaga acgagggccc tgattcgtgg tcatcgtcga tcctcggagg cgcccgccga
     5461 tccggcgccg gatgtcggcg cacgactccg cgtagccgat gtcgcgcgcc ccggccgcgc
     5521 gcaaggaaga gccaccggcg tgggcggggc
//
LOCUS       DASVES010000043        17638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_150685, whole genome shotgun sequence.
ACCESSION   DASVES010000043 DASVES010000000
VERSION     DASVES010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150685"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1652)
                     /locus_tag="VF240_01715"
     CDS             complement(102..1652)
                     /locus_tag="VF240_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173988.1"
                     /translation="MPLQLRRRLAVPALVLIVLCMAVPARPQGASRRAEDVWGPSYKG
                     SSAQPARRRYPSPQDTLSRLRHWNEAAVNSSGLDHTPVPVGDPRIFGEQIGPARSSRA
                     IAIVHVAVFEAVNAIKGGYRSYVGLPRANPSASVDAAVAQAAHDTLNALFPSQAASCT
                     ALLVEDLGHIKDGPQKEAGIAVGQQAAAATLALRSGDHSLHAEPRVGIEFITSDDPGW
                     WRQDPISMIPLALGAHWSQVTPFVMQSASQFRTPPPPSMDSPEYAAAFDEVKRLGGDG
                     LTTLTERTPEQTDIGIYWAYDGTPSLCAPPRLYNQIAVQIAGQMGSNFVETARLLALL
                     NLALADAGVASWESKYFYQVWRPVTGIREADAGTGPTGLGDGNPATVGDPTFSPLGAP
                     ASNLTAGLNFTPPFPAYPSGHAVFGGALFETLRNFYGRDDIAFTFVSDELNGVTVGND
                     GVVRSLSPRSFSSLSQAEEENGQSRIYLGIHWSFDKTEGIAQGRRVADYVFENAFAPV
                     RGANAHRK"
     gene            2210..3046
                     /locus_tag="VF240_01720"
     CDS             2210..3046
                     /locus_tag="VF240_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7173989.1"
                     /translation="MSRNKYLMAGAVLLVIVLLVGASVAFANIQYAQEGTVQVVTKWG
                     AIERVYKPSDGWFTTIAPGRKSYEVNIKSFTETAPVRVTSRDNAALQVEIAVTAYTDA
                     ENVADYVRKYGFTEDERHRRRSEILKGLVQTEARNSFADYGAYEIYANQELIQKRIVE
                     SLRPQLAGQLLLVTESVQIGNPDFIDDRIEAAASGVVANEKQKQAEEARLEAAKIAAQ
                     TKQIEALTYANPALLDIKKLELQLEIERARADGIRNHQGALTIMYGAGNPGVQMQVPT
                     GR"
     gene            3053..3205
                     /locus_tag="VF240_01725"
     CDS             3053..3205
                     /locus_tag="VF240_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173990.1"
                     /translation="MRIIILLWAAVEVLQIVVIGVLLVGYRNMQRRLDGGDHFAASPP
                     RSVTEE"
     gene            complement(3320..3934)
                     /locus_tag="VF240_01730"
     CDS             complement(3320..3934)
                     /locus_tag="VF240_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEX7173991.1"
                     /translation="MKDIAFKKVLVFVNAGVPLLLLLWDWWRGQLGADPLNFVTRSTG
                     TLTLVFLLLTLCVTPARKITGVQWLIRFRRMLGLYAFFYGFVHFLCYLWFDKAFQLGE
                     VVADVTRRRFILVGMLGFFLMAPLAITSTNAMIKRLGGKRWARLHKLIYVSAVAGVFH
                     FYLLVKADTTKPLIFALVLAALLGYRLMVSQQQKTPLRLGVSDR"
     gene            complement(4039..4998)
                     /gene="msrP"
                     /locus_tag="VF240_01735"
     CDS             complement(4039..4998)
                     /gene="msrP"
                     /locus_tag="VF240_01735"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX7173992.1"
                     /translation="MKSEPIKSSEITPKSVYLNRRLFMQGAALAVSTAATGLLYKQLL
                     APATKEPAPESIRTGATEGRWDLPGEPATPYDDITSYNNFYEFTTSKEGVAEKSKGFV
                     TRPWTVSVEGLVNKPKVFDIDDLLRLAPQEDRIYRLRCVEGWSMVIPWLGFPLARLLE
                     QAEPQAGARFVAFQTLLDPKRLPNQRSRVLDWPYVEGLRLDEAMHPLTTLATGLYGEP
                     LPPQDGAPIRLVVPWKYGFKSIKSIVKITLVERQPPTTWNIAASDEYGFYSNVNPEAP
                     HPRWSQASERRIGEFGRRPTLLFNGYADEVAHLYAGMDLRKNF"
     gene            5333..6058
                     /locus_tag="VF240_01740"
     CDS             5333..6058
                     /locus_tag="VF240_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173993.1"
                     /translation="MKLNKIISAFAAIIIAASTAFGAPAAVENNADGNGAGRVRVPLN
                     LAVLIQDDVTQRVGNELERTREFIRRLPAGSRVMVGYVTSGSLQVRQSFTTDLESAAK
                     SLRLPVGNEAASPYNPFVEVREALKKFPTDGKNFNALLLVSDGLDVSRGFGPSTFVHS
                     IDLESAVREAQRRGVAVYTFYAPTQLTASSRTAANRGQSALNRLTKETGGQAFFQGTD
                     FVSFDPYFRDFSRTLNRQFAGAI"
     gene            6325..6666
                     /locus_tag="VF240_01745"
     CDS             6325..6666
                     /locus_tag="VF240_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173994.1"
                     /translation="MTAHQEYCLIVEGNYLSESEAEHALRDPFIEDWVEETGRFRLHN
                     LGEIILTPGVTLGSLGVLMLDDGVFEIASTDPEHPLTEHKAKGVADALRRQDMFDIIE
                     VEPRGGEEETP"
     gene            6858..7406
                     /locus_tag="VF240_01750"
     CDS             6858..7406
                     /locus_tag="VF240_01750"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX7173995.1"
                     /translation="MAMFLGGRVKSITGDITVQEVDAIVNAANSTLLGGGGVDGAIHR
                     AGGPAILEECREIRRTRWPSGLPAGEAVITTAGRLPARHVIHTVGPVWGSEDGREAEL
                     LAACYRNSLALARARGLDSIAFPAISTGAFGYPTDEAAAVASKTIEEFLSENESPREV
                     RLVFFSPAGAEDFLKHQKFSER"
     gene            7502..9664
                     /gene="glgP"
                     /locus_tag="VF240_01755"
     CDS             7502..9664
                     /gene="glgP"
                     /locus_tag="VF240_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX7173996.1"
                     /translation="MILPMKEMERETAGESGERELPETLGALGRISWNYWWSWAPDGG
                     AVMRDLDPEVWEECGHNPRRLLAETPEFNLLKMATEPRYVERVARLAERFDHYTSATT
                     DTWAARHAVEITHERPVAYFCAEFGVHNSLPLYSGGLGVLAGDHLKSASDLGLPLVAI
                     GLLYHHGYFHQRLRRDGWQEETYRQIHLPHLPLTLVRGDDGAPLQIEMTIRGRRVLTR
                     AWRAQVGRVALHLLDTNIAENHEIDRLITGHLYGGDRETRCVQEMVLGIGGVRLLRRL
                     GVEPHVFHLNEGHSAFLTLELARELTSGGVRFGEAAHAVKSRCVFTTHTPIAAGHDEF
                     PASLVEKCFGEGYWERLGLAREEFLNLGRVVADDDAELFGLTPLALRMCRSSNGVSRK
                     HGEVSRELWMKMWPGRHADEVPITHVTNGVHAPTWVSPLLRSVYEKHVGDDWADLLLD
                     PERWAERVGSIPDEELWGAHRLLKRRLVAFVRQRLYHQRVQQGEEMEFAEAARKVLDP
                     EALTVGFARRVAGYKRWSLILTDYERLHRMMNDPARPLQFVFAGKAHPQDQGAKFILQ
                     QIALWKLDPRISARAVFLQDYDHEVARQMVQSVDVWLNVPRRPLEASGTSGQKVAMNG
                     GLNLSVLDGWWPEGFDTTNGWTVGDAAPGDGDDTDLRDAESLYHVLEAQIVPAFYERD
                     EKGVPRRWVAMMRHAIQTLAPAFNSDRMVREYAKNIYA"
     gene            9801..12527
                     /locus_tag="VF240_01760"
     CDS             9801..12527
                     /locus_tag="VF240_01760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX7173997.1"
                     /translation="MAPTIEAALTALREHFGFEGFREGQREVIESILEGHDTVVVMPT
                     GGGKSLCFQLPAMMREGATLVVSPLIALMKDQVDALHARGLPATFINSSIDFEEQRAR
                     ITGMREGRFKLVYVAPERFRSQHFVETLLRAGVSLFAIDEAHCISQWGHDFRPDYLRL
                     KSFLEQIGRPQTVALTATATPYVRADIIEQLSLRDPRAFVSGFDRPNLSIGVVHTQKE
                     RDKTARVKALANAYEGGSGIVYSSTRKSVEQVAQRMKEARLSVVAYHAGMADDERVRA
                     QDDFMSGRVQMIVATNAFGMGIDKPDIRFVAHHHLPGSIEAYYQEIGRAGRDGLNSTC
                     MLLFNYADKRTQDYFIEGSYPPPEVIAKVYEALVGTGQRRIELSTREIAARAGVRNEM
                     AVQSSLVVLEKAGHVERGAGAENRATVRLLVSAQEARDAANSRRSALLKQGLLGLLGG
                     FDLSQRAETDIDVAEMGEIAGLDVQGARRALGALAEAGLITYQPARRTRGVQLLDERP
                     VSNLRIRPQELARRAALEQRKLREMISFCYTDRCYRAFILDYFGDSSYVGNCGKCGNC
                     LAATASHAGAATAAGAGLASAPPAGAPLDDPTSLDRFMMKHVPVGLELEDELGERKRV
                     RRAGVEAERRAAAFDDDGADRPVVVTEQRELLAEEMLYVRKILACAARMQGRFGKNLL
                     AATLRGSRSAKITQAGLDELSTFGILSGMTQDEILLYVDALVSAGCLHVTAGAYPLVE
                     LTEAGGEVMRERAEVRLALPAPRPPATRYETTPTAYARPRESRAAARGDAPVVPRRST
                     MDETYDLYCQGVTIEEICRQRGLTEMTVEKHLADCIVEGRAFDISPHVPAETRALIEA
                     AAERIGVERLKPLRDELPRHVTYRMIRFVVADLQRATPDESGVSARGDNEPL"
     gene            complement(12749..13153)
                     /locus_tag="VF240_01765"
     CDS             complement(12749..13153)
                     /locus_tag="VF240_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173998.1"
                     /translation="MNTIVVTYLVYLMVSVGLTVWVAQTLHKNGRVFLVDVFHGNESL
                     ADSVNHLLVVGFYLINFGYVTLALKIGWAVVDARASIEALSWKIGLVLLVLGAMHFFN
                     LLVFSRIRQRAVYGFNPARPGVYKGFREETGA"
     gene            13372..13590
                     /locus_tag="VF240_01770"
     CDS             13372..13590
                     /locus_tag="VF240_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7173999.1"
                     /translation="MSREPLDHPTDPTGRPEPPPAQPHDPATPDPSKPEPLPLPPDAP
                     TPRAPVEEPGPAPLPAGDPPPLEPTRLA"
     gene            13639..14769
                     /locus_tag="VF240_01775"
     CDS             13639..14769
                     /locus_tag="VF240_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7174000.1"
                     /translation="METTTTTAGTSSPRRAEEDARESDSRVRPQGRASRLQDIARALE
                     QRPFTPHTLFRGGHAQTLRGALRWPRRLRMLREYAGAERRLFDIEPGVRLLAHCRWQT
                     RKTDHPTLVLVHGLEGSSRSVYMLGTGRKAFRAGFNVVRLNVRNCGGTESLSPTLYHS
                     GMSGDLLRVFAELFEHDRLPEIFLAGFSLGGNLVLKLAGELGADAPGALAGVAAVSPS
                     LDLSASADAIERPQNRFYQWSFIRSLRRTLRRKHKLFPHLYDLSRLREMRTIRQFDDF
                     YTAPHGGFRDSADYYARSSALALAARIRVPTLIIHAHDDPFIPYAPLRNRAISDNPDI
                     LLLDPPRGGHVAFVGSARRGEDRFWAENRVVQFCTLLSRGGR"
     gene            14875..16245
                     /locus_tag="VF240_01780"
     CDS             14875..16245
                     /locus_tag="VF240_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7174001.1"
                     /translation="MITALKLFAVVIIVLVNGFFVAAEFAFVGVRRGRIEAEAEAGNK
                     RARRLLGILGNLNAYLSAAQLGITLASLALAILGEPTIASLIEGPIARTLEGRLSAEM
                     VETITHGLAFAIAYSILTSLHITVGEQAPKLFGLERAERVAMFSALPMEVFYKTFRWP
                     IHALDWASAAMVRLFGLHASSAHASIYTQEEIRQIIEMSHKGGHVEADEQRLLNRVLD
                     FTDAEVREAMVPRTAVAALSVTATLEEAEAAFCEGGYSRLPVYRERLDEVVGVLFMKD
                     LMPCLRRLDPAEFDMERALHPPLFVPAAARLGSVLAQMQSAQTHLACVVDEHGGLEGI
                     VTLEDLLEEIVGEINDEFDEESRSQITEESGGTYLLDGMLAVRDANRRFQLKLDEEAG
                     YTTVAGFLLAKSGRLLRPGEVVEHDGARFQVETVDRRRIRRIRYTPAAAAPGPEDEEA
                     ASRTHA"
     gene            16269..16439
                     /locus_tag="VF240_01785"
     CDS             16269..16439
                     /locus_tag="VF240_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174002.1"
                     /translation="MSDNATTELIIVLAVMLLLFIFGLAAVVIFWRVWRREQKSRVDG
                     DSADEPRSGGRM"
     gene            complement(16656..17429)
                     /locus_tag="VF240_01790"
     CDS             complement(16656..17429)
                     /locus_tag="VF240_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174003.1"
                     /translation="MTGLMLTAALVVSGAASASRAQKKTTERPSATAGDGRSAQQPSA
                     SPAKTAGDTVAAGAAEVEEARYRYEFENEKFVVSRIRIVHDAAGRGMLTFERKSSEEA
                     IEEPLELSAVALRRIRGHWEALRFLDSEASYQADKQFPHLGTVRLSMARGARNRTTEF
                     NYSRDPDASALASEYRRAADQAMFVFDINVARDNQPLETPKLLEYLGRLVERGGVSDP
                     QQLAPLLKDLSTDERVPLITRNTAGKILKKLEKAAKPHK"
     gene            complement(17447..>17638)
                     /locus_tag="VF240_01795"
     CDS             complement(17447..>17638)
                     /locus_tag="VF240_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174004.1"
                     /translation="DEVDAPLDEANIGRFTAKIAQMSEETQFIVITHNKRTMETARAL
                     YGVTMEEVGISKLVSVRFE"
BASE COUNT         3044 a         6026 c         5656 g         2912 t
ORIGIN      
        1 agcggtagcg agcgggtgtg cctacaatga cagacccgcc ccgcgcaagc gagacccgcg
       61 aggcttccgc tcgcggtact gtttatgatg ataaggccga ttcacttccg gtgtgcgttc
      121 gccccgcgca ctggcgcgaa cgcgttctcg aagacgtagt cggccacgcg ccggccctgg
      181 gcgatgccct ccgtcttgtc gaacgaccaa tggatgccta agtagatgcg gctctggccg
      241 ttctcctcct cggcctgcga cagcgacgag aagctgcgcg gcgagagaga gcgcaccacg
      301 ccgtcgttgc cgaccgtgac gccgttcaac tcgtccgaga cgaaggtgaa ggcgatgtcg
      361 tcgcgcccgt agaagttgcg cagggtctcg aagagggcgc cgccgaagac ggcgtgcccc
      421 gaagggtagg ccgggaaggg cggcgtgaag ttcagacccg ccgtgaggtt gctggccggt
      481 gcgccgagcg gcgagaaggt cgggtcgcct acggtcgcgg ggttgccgtc gcccaggccg
      541 gtcggccccg tccccgcatc ggcctcgcgg atgccggtca cgggtcgcca gacctggtag
      601 aagtatttcg actcccacga cgccacgccg gcatcggcca acgccagatt gaggagcgcc
      661 agcagccgcg ccgtctcgac aaagttcgac cccatctgcc cggctatctg cacggcgatc
      721 tgattgtaga ggcgcggcgg cgcgcacagc gagggcgtcc cgtcgtaggc ccagtagatg
      781 ccgatgtcgg tctgctcggg cgtgcgctca gtcagggtcg tcagcccatc gccgccgagg
      841 cgcttcacct cgtcgaaggc cgccgcgtac tcggggctgt ccatcgatgg cggcggcggg
      901 gtgcggaact gcgaggcgga ctgcatgacg aagggagtga cctgactcca gtgtgcgccg
      961 agcgcgagcg ggatcatgct gatcgggtcc tggcgccacc aaccggggtc gtcgctcgtg
     1021 atgaattcga tgccgacgcg cggctccgcg tgcagcgagt ggtcgccgga gcgcagcgcc
     1081 agggtcgcgg cggccgcctg ctggccgacg gcgatgccgg cctccttctg cggcccgtcc
     1141 ttgatgtgtc cgaggtcttc gacgagcagg gcggtgcagc tggcggcctg cgaggggaaa
     1201 agcgcgttta gcgtgtcgtg cgccgcctgc gcgacggcgg cgtccaccga ggcgctgggg
     1261 ttagcccgag ggaggccgac gtaactccgg tagccgcctt tgatggcgtt gacggcctcg
     1321 aagacggcga cgtggacgat ggcgatggcg cggctggagc gggccggccc gatctgctcg
     1381 ccgaagatgc gtgggtcgcc gacggggacg ggcgtgtggt cgaggccgct ggagttgacg
     1441 gcggcctcgt tccagtggcg gagtcggctc agcgtgtcct gcggcgaagg gtatctccgg
     1501 cgggcgggct gagccgacga acccttgtag ctcggccccc acacgtcttc ggcgcggcgc
     1561 gaggctccct gcggccgggc ggggacggcc atgcagagga cgattaagac caacgctggg
     1621 acggccagcc tccgtcttag ttgcaaaggc ataactactc ctctcaagag agtgacggct
     1681 cggtgagaac ttatccgagg cgcttcagag tcaggggaga ctgagagcgg ttcggggccg
     1741 aaggcgccgg cggcgaaggt cgccgcggtc agcgcgccgg cccggtcgag agactggcgc
     1801 cgtttggtgg cgtgcggggg atgattgttg tccatgaaag tcttccttga agtttcgggc
     1861 cggagatttg aaagagcgag gacggtattt tgcgccggcg gaaagtgcgg cgcgtcgtga
     1921 gatgattaag tcgtgaaggc gttcaccggg agcaagtgcc gcctctgccg ggcaagcgat
     1981 tagaaagact ggcagcggcg cggaagatac accggcgggg gccggaagtc aaaataaaaa
     2041 gcccgacgcg cgcccgtccg tcgtccccac acagagcggc ccacgagcgc ggccgtggcc
     2101 ggagcgcatt cgcacaaccc gccggcgcat ttttacgtat aatcaactga tgaaaaaagt
     2161 gaataaagaa gaatggcccg aaacagttat cgccaacaaa ggagaattga tgtcgcgtaa
     2221 caagtatctg atggcgggcg ccgtgctgct ggtcatcgtg ctgctggtcg gagcgtcggt
     2281 cgccttcgcc aacatccagt acgcacagga gggcaccgtc caggtcgtca ccaagtgggg
     2341 cgccatcgag cgcgtctaca agccctcgga cggctggttc acaaccatcg cgccgggccg
     2401 gaagtcttac gaagtgaata tcaaatcctt caccgagacc gcgcccgtgc gcgtgacgag
     2461 cagggacaac gcggcgctcc aggtggagat cgccgtcacg gcctacacgg acgccgagaa
     2521 cgtcgccgat tacgtgcgca agtacggctt cacggaggac gagcggcacc gccggcgcag
     2581 cgaaatcctc aagggactcg tgcagaccga ggcgcgcaac tccttcgccg actacggcgc
     2641 ttacgagatt tacgccaatc aggagctgat tcagaagcgc atcgtcgaga gcttgcgccc
     2701 gcagctcgcg ggccaactgc tcctcgtcac cgagtcagtg cagatcggta accccgactt
     2761 catcgacgac cgcatcgagg ccgcggcctc gggcgtggtc gccaacgaga agcagaagca
     2821 ggccgaggag gcgcggctcg aagcggccaa gatcgccgca cagaccaagc agatcgaggc
     2881 gctgacctac gccaaccccg ccctcctcga catcaagaag ctggagcttc aactcgaaat
     2941 cgagcgggcg cgcgccgacg gcatccgcaa tcaccagggc gcgctcacta tcatgtacgg
     3001 ggccggcaac cccggcgtgc agatgcaggt tccaacgggg aggtaacgac cgatgagaat
     3061 aattattctc ttgtgggccg cggtcgaggt gttgcagatc gtcgtcatcg gcgtgctcct
     3121 tgtcggctac cgcaacatgc agcgcagact cgacggcgga gatcacttcg ccgccagccc
     3181 tccacgctcc gtgactgaag agtgacgagc cggtagcgga tgggcagcga cagaaaagac
     3241 aaagggcgcg cggcggatac gaaatccgcc gcgcgccctt ttgtcgtggc cacttggtgc
     3301 tcggagcgac gcggaagcgt cagcggtcgg agacgccgag cctcagcggc gtcttctgct
     3361 gttgggagac catcaacctg tagccgagga gcgcggcgag caccagcgcg aagatgagcg
     3421 gcttcgtcgt gtcagccttc accagcaggt aaaagtggaa gacgcccgcc acggccgaga
     3481 cgtagatgag tttgtggagc cgcgcccaac gcttgcctcc gagtcgcttg atcatcgcgt
     3541 tggtcgaggt gatggcgagc ggcgccatca ggaagaagcc gagcatgccg acgaggatga
     3601 atctgcggcg ggtgacgtct gcgacgacct cgccgagttg gaacgccttg tcgaaccaga
     3661 ggtagcagag gaaatgtacg aagccgtaga agaaggcgta gagaccgagc atgcgccgga
     3721 agcggatgag ccactgcacg ccggtgatct tgcgcgcggg ggtgacgcag agcgtcagca
     3781 gcaggaagac gagcgtgagc gtccccgtcg agcgcgtcac gaagttgagc gggtcggcgc
     3841 cgagctgccc acgccaccag tcccacaaca gcagcagcag cggcaccccc gcgttgacga
     3901 agacgagaac ttttttgaag gcgatgtctt tcacagttgc gaaggagtca gaagtcagaa
     3961 gtcaggagtc agaatacgaa ctagtatccg ttccgctttt attctgactc ctgacttctg
     4021 acttctgact cccttctcct aaaagttctt cctcaaatcc atccccgcgt agaggtgtgc
     4081 gacttcgtcg gcgtagccgt tgaagaggag cgtggggcgg cggccgaact ctccgatgcg
     4141 gcgctcgctc gcctggctcc agcgcgggtg cggcgcctcc gggttgacgt tcgagtagaa
     4201 gccatactcg tccgaggcgg cgatgttcca cgtggtcggc ggctggcgct cgacgagggt
     4261 gatcttgact atcgacttga tgctcttgaa gccgtacttc cacggcacga ccagacggat
     4321 gggcgcgccg tcctgcggcg gcagcggctc gccgtagagg ccggtggcga gggtggtgag
     4381 cgggtgcatg gcttcgtcga gtcgaaggcc ctcgacgtag ggccagtcga gcacgcggct
     4441 gcgctggttg ggcaggcgct tcgggtcgag caaggtctgg aaggcgacga agcgcgcgcc
     4501 cgcctgcggc tccgcctgtt cgagcaggcg cgccagcggg aagccgagcc acgggatgac
     4561 catcgaccag ccctccacgc agcgcaggcg ataaatcctg tcctcctgcg gcgcgaggcg
     4621 aagaaggtcg tcgatgtcga agaccttcgg cttgttgacc aagccttcga ccgagaccgt
     4681 ccacgggcgc gtgacgaaac ctttcgactt ctcggcgacg ccctccttgg aggtcgtgaa
     4741 ctcgtagaag ttgttgtagc tcgtgatgtc gtcgtagggc gtggccggct cgcccggcaa
     4801 atcccaacgc ccctcggtcg cgccggtgcg gatgctctcg ggcgcaggct ctttcgtcgc
     4861 cggcgcaagc agttgcttgt agagaaggcc ggtggccgcc gtcgagacgg cgagcgccgc
     4921 cccctgcatg aagaggcgac ggttgaggta aacgctcttt ggcgtgatct cgctgctttt
     4981 aatcggctcc gacttcatga tcgtcaaatc ctgcccttac gccggtaatg cgttcggccg
     5041 cgcgccctcc caaacaatct acgcgcgacg cgcgccccgc agtttgccaa cgcgaaataa
     5101 aaactttccg cagccattct actcttctga ttcgctgacc cgttaggccg cttaagagtc
     5161 gcggcctccg ccggtggtcg cgcctcccgc ccacgcgcaa aaagcttgtc gcgtcccggt
     5221 cttctccacc gcacaaatcc tgtcggcggg ccacctcgcg aagaaaatgg gcatcacagt
     5281 acgcggataa aaactgccaa taacggcaat cggaacacag gggaagtaag ttatgaaatt
     5341 gaacaaaatc atctccgctt ttgcggcaat catcatcgcc gcttcgaccg ccttcggcgc
     5401 gccggcggca gtcgagaata acgccgacgg caacggggcc gggcgcgtcc gcgtcccctt
     5461 gaacctcgcc gtgctcattc aggacgacgt gacgcagcgc gtcggcaacg aactggagcg
     5521 cacgcgcgag ttcatccggc gcctccccgc gggttcgcgc gtgatggtcg gctacgtcac
     5581 ctcgggcagc ctacaggtgc gacagtcatt cacgaccgat ctggagagcg cggccaagtc
     5641 actgcgtctc cccgtgggca acgaagccgc ctcgccctac aacccgtttg tcgaggtgcg
     5701 cgaggcgctc aagaagttcc cgaccgacgg gaagaacttc aacgccctgc tcctcgtctc
     5761 cgacggcctc gacgtgtcgc gcggcttcgg cccttcgacc ttcgtccact ccatcgatct
     5821 cgaaagcgcg gtgcgcgagg cgcaacgccg cggcgtggcc gtctacacgt tctacgcccc
     5881 gacgcagctc accgcgtcga gccgcacggc cgccaaccgt ggccagagcg cactcaaccg
     5941 tctgacgaaa gaaaccggcg gccaagcctt cttccagggc accgacttcg tcagcttcga
     6001 cccctacttc cgcgacttca gccgcacgct caatcggcag ttcgccggcg ccatctgacg
     6061 ccgggagcgc cgacaatgag gggcgtatga cagcctccgc cacacgccag cttcagtaat
     6121 tcaacgacgc cgccgagggc ctacctgccc cggcggcgtc tgtctttttc agcctctcgc
     6181 ttgcgcgcgg cgcgcgggca aacaaaatta tctaccgcgc gcgaaaaagt tttgtcaccc
     6241 cgcgcggggc ggtgctaaag tgagcgggcc gaagccttgc accggcccgc ctgcgggtgg
     6301 tctcgctgct tcaccccaac cgtcatgacg gcacaccaag agtactgcct gatcgtcgag
     6361 ggcaactacc tttcagaatc agaggcggag cacgccctgc gcgatccctt catcgaggat
     6421 tgggtcgagg agacaggacg cttccgcctg cacaacctcg gcgagatcat cctgacgccc
     6481 ggcgtcacgc tcggctccct cggcgtgctg atgctcgacg acggcgtctt cgagattgcc
     6541 agcaccgacc ccgaacaccc gctcaccgaa cacaaggcca agggcgtggc cgatgccctg
     6601 cggcgacagg acatgttcga catcatcgag gtcgaaccac gcggcggcga ggaagagacg
     6661 ccttaaaggc cgtgaatcgt caatcgtcaa tcgtgaatcg aaggggttga acttcatccg
     6721 acttccgatt ggacgtttaa gctgagtcag gttcaaccac gattcacgat ttacgattta
     6781 ccatttacga ttcacgattc acgtcccggc tttcatcttc cgcacgcatg atggtaaatt
     6841 cgtcgcccca acaagctatg gccatgttcc tcggcgggag agtcaagagc atcaccggcg
     6901 acattacggt tcaggaagtt gacgccatcg tcaatgcggc caacagcacg cttctgggcg
     6961 gcggcggcgt tgacggagcc atccaccgcg cgggcggccc cgcgattctg gaggaatgcc
     7021 gcgagattcg gcgcacgcgc tggccctcgg gtctgccggc gggcgaggcc gtcatcacca
     7081 cggcaggccg tctcccggca cgccacgtca tccacacggt cggccccgtc tggggaagcg
     7141 aggacgggcg cgaggccgaa ctcctcgccg cctgctatcg caactcgctg gcgctcgccc
     7201 gcgcgcgcgg cctcgactca atcgccttcc ccgccatctc gaccggcgcc ttcggctacc
     7261 cgaccgatga ggccgcggcc gtcgcctcga agacgattga agagttcctt tctgaaaatg
     7321 agtcgccgcg cgaagtgcgc ctcgtcttct tctcgccggc gggcgccgaa gattttctca
     7381 agcaccaaaa atttagtgaa aggtagtcag gagtcaggag tcaggagtca gaatgaagaa
     7441 gcgaagagag gttctgtttt tcattctgac tcctgactcc tgactcctga cttctggtta
     7501 aatgattctt ccgatgaaag agatggagcg tgagacggcc ggcgagtccg gcgagcgcga
     7561 gctgcccgag acgctcgggg cgctggggcg gatttcgtgg aactactggt ggagctgggc
     7621 gcccgacggc ggggccgtga tgcgcgacct cgacccggag gtctgggagg agtgtggaca
     7681 caacccgcgt cgcctgctcg ccgagacgcc cgagttcaac cttttgaaga tggcgaccga
     7741 gccgcgctac gtcgagcgcg tcgcccgtct ggccgaacgc ttcgaccact acacgagcgc
     7801 gacgaccgac acgtgggccg cgcgccacgc cgtcgagatc acgcacgagc gccccgtggc
     7861 ctacttctgc gccgagttcg gcgtccacaa ttcgctgccg ctctactccg ggggcttggg
     7921 cgtgctcgcc ggcgaccacc tgaagtcggc gagcgacctc ggcctgccgc tcgtcgccat
     7981 cgggctgctt tatcatcacg gctatttcca ccagcgcctc cgccgcgacg gctggcagga
     8041 ggagacctac cggcaaattc acctcccgca tctcccgctc acgctcgttc gcggtgacga
     8101 cggcgcgccg ctccagatcg agatgaccat ccgcggccgg cgcgtgctca cgcgtgcgtg
     8161 gcgcgcacag gtcggacgcg tcgcgctcca cctgctcgac accaacatcg cggagaacca
     8221 cgagatcgac cgcctcatca ccggccacct ctacggcggc gaccgcgaga cgcgctgcgt
     8281 gcaggagatg gtcttaggca tcggcggcgt ccgcctgctg cgccgcctcg gcgtcgagcc
     8341 gcacgtcttc cacttaaacg agggccactc ggccttcctg acgcttgagc ttgcgcgcga
     8401 gctgacctcg ggcggcgtcc gcttcggcga ggccgcccac gccgtcaaga gtcgctgcgt
     8461 gtttacgacg cacacgccca tcgccgccgg ccacgacgag ttccctgcct ccctcgtcga
     8521 gaagtgtttc ggcgaaggct actgggagcg tctgggcctc gcgcgcgaag agttcctcaa
     8581 cctcgggcgc gtcgtggccg acgacgacgc ggagctgttc ggtctgacgc cgctcgcgct
     8641 ccgcatgtgt cgctcgtcga acggcgtgag ccgcaagcac ggcgaagtgt cgcgcgagct
     8701 gtggatgaag atgtggcccg gacgccacgc cgacgaggtg cccatcacgc acgtcaccaa
     8761 cggcgtccac gcgccgacgt gggtctcgcc gctcctgcgc tccgtctacg aaaagcacgt
     8821 gggcgacgac tgggccgacc tgttgctcga ccccgagcgt tgggccgagc gcgtcggctc
     8881 gatccccgac gaggaactgt ggggcgcgca ccgccttctg aagcgccggc tcgtcgcctt
     8941 cgtccgccag cgcctctatc accaacgcgt gcagcagggc gaggagatgg agtttgccga
     9001 ggccgcgcgc aaggtgctcg acccggaggc actgaccgtc ggcttcgccc gccgggtcgc
     9061 cggctacaag cgctggagtc tcatcctcac cgactacgag cgcctgcacc ggatgatgaa
     9121 cgaccccgcg cgccccttgc agttcgtctt cgccggcaag gcccacccgc aggatcaggg
     9181 ggccaagttc atcctccagc agattgcgct gtggaagctc gacccgcgca tcagtgcgcg
     9241 cgccgtcttc ttgcaggact acgatcatga ggtggcccgc cagatggtgc agtcggtgga
     9301 cgtgtggctg aacgtcccgc gccggccgct ggaggcgtcg ggcacgagcg gccagaaggt
     9361 ggcgatgaac ggcggcctca acctctccgt cttagacggc tggtggcccg aaggcttcga
     9421 cacgaccaac ggctggacgg tcggcgacgc cgcgcccggc gacggcgacg acacagacct
     9481 ccgcgacgcc gaatcgctct atcatgtact ggaggcgcag atcgtccccg ccttttatga
     9541 acgcgacgag aagggcgtgc cgcgccgctg ggtcgccatg atgcgccacg ccatccagac
     9601 gctcgccccc gccttcaaca gcgaccgcat ggtgcgggag tacgcgaaga atatctacgc
     9661 ctgaacgcgc gcgaccgcga cagtaccgcg agcggtagcg agcgggtgtc gccgccgagg
     9721 gacgcgaccc gctcgctacc gctcgcggta ctgttttcgc cgcttgtcac ttcccgcgcg
     9781 ccgtcttaga cttttgactt atggctccga caatcgaggc ggccctcacc gcgctccgtg
     9841 aacatttcgg cttcgagggt ttccgagaag gccagcgtga ggtcatcgag tcgatactcg
     9901 aaggccacga caccgtcgtc gtgatgccga cgggcggcgg caagtccctc tgcttccagc
     9961 tccccgcgat gatgcgcgag ggcgccacgc tcgtcgtctc cccgctcatc gccctgatga
    10021 aagatcaggt ggacgcgctc cacgcgcgcg gcctgcccgc caccttcatc aacagctcca
    10081 tcgacttcga ggagcagcgc gcgcgcatca cggggatgcg cgagggtcgc ttcaaactcg
    10141 tctacgtcgc acccgaacgc ttccgcagcc agcacttcgt cgagacgctt ctgcgcgccg
    10201 gcgtctccct cttcgccatc gacgaggccc actgcatctc gcaatggggc cacgactttc
    10261 gccccgacta cctgcgcctc aagtcgttcc tcgaacagat cggccgcccg cagaccgtgg
    10321 cgctcacggc gacggccacg ccgtatgtcc gcgccgacat catcgaacaa ctttccttac
    10381 gcgacccgcg cgccttcgtc tccggcttcg accgccccaa cctctccatc ggcgtcgtcc
    10441 acacgcagaa ggagcgcgac aagacggcgc gcgtcaaggc gctcgcgaac gcttatgaag
    10501 gcggctccgg catcgtctat tcctcgacgc gcaagtcggt cgagcaggtg gcgcagcgga
    10561 tgaaggaagc gcgtctctcc gtcgtcgcct atcacgccgg aatggcggac gacgagcgcg
    10621 tgcgcgcgca ggacgacttc atgtcgggcc gtgtgcagat gatcgtcgcc accaacgcct
    10681 tcggcatggg catcgacaag cccgacatcc gcttcgtcgc gcaccaccac ctgcccggct
    10741 ccatcgaagc ctactatcag gagatcggcc gcgccgggcg cgacggcctg aactcgacct
    10801 gcatgctcct gttcaactac gccgacaagc gcacgcagga ctacttcatc gaaggctctt
    10861 acccgccgcc cgaggtcatc gccaaagtct acgaggcgct cgtcgggacg ggtcagcgcc
    10921 gcatcgaact ctcgacgcgc gagatcgcgg cgcgcgccgg cgtccgtaac gagatggccg
    10981 tgcaatcgtc gctcgtagtg ttggagaagg ccggccacgt cgagcgcgga gccggcgcgg
    11041 agaaccgcgc gaccgtgcgc ctgctcgtct ccgcgcagga ggcgcgcgac gccgccaact
    11101 cccgacgcag cgcgctcctc aagcaggggc ttctgggcct gctcggcggc ttcgatctca
    11161 gccagcgcgc cgagaccgac atcgacgtgg cggagatggg cgaaatcgcc ggcctcgacg
    11221 tgcagggcgc gcgacgcgcg ctcggcgcgc tcgccgaggc cggactcatc acgtaccagc
    11281 cggcgcggcg cacgcgcggc gtccaactcc tcgacgaacg ccccgtcagc aaccttcgca
    11341 tccgcccgca ggaactcgcg cgccgcgccg cgctcgaaca gcgcaagctc cgcgagatga
    11401 tctccttctg ctacaccgac cgctgctatc gcgccttcat cctcgactac ttcggcgaca
    11461 gctcctatgt gggcaactgt ggcaagtgcg gcaactgtct cgccgccacc gcgagccacg
    11521 ccggggcggc gacggcggcc ggcgcgggtc tcgcgagcgc gccgccagca ggcgcgccgc
    11581 tcgacgaccc cacctcgctc gaccgcttta tgatgaagca cgtccccgtc ggcctcgaac
    11641 tcgaagacga gctgggcgag cgcaagcgcg ttcgtcgcgc gggggtcgag gctgaacgac
    11701 gcgccgccgc gttcgacgat gacggcgcgg acaggcccgt cgtcgtcacg gagcagcgcg
    11761 agcttctggc cgaagagatg ttgtacgtgc gtaaaattct cgcgtgcgcc gcgcggatgc
    11821 aggggcgctt cggcaagaac cttttggccg ccacgcttcg cggctcgcgc tcggccaaga
    11881 tcacgcaggc cggactcgac gagctttcca ccttcggcat cctctccggc atgacgcagg
    11941 acgaaatcct gctctacgtg gacgctctgg tctccgccgg ctgcctccac gtcaccgccg
    12001 gcgcctatcc cctcgtcgaa ctcacggagg ccggcggcga ggtgatgcgc gagcgcgccg
    12061 aagtccgcct cgccctcccc gcgccccgcc cgccggcgac gcgttatgag accacgccga
    12121 cggcctatgc ccgtccccgc gagtcgcgtg cggcagcgcg cggcgacgcg cccgtcgtcc
    12181 ctcgacgcag cacgatggat gagacttacg atctttactg tcagggagtc actatcgaag
    12241 agatttgccg gcagcgcggg ctaacggaga tgacggtcga gaagcatctg gccgactgca
    12301 tcgtcgaggg acgggcgttc gacatctcgc cgcatgtccc ggccgagacg cgcgcgctca
    12361 tcgaggcggc tgctgaaaga atcggcgtcg agcgtctcaa gcccctgcgc gacgaactcc
    12421 cgcgccacgt cacctaccgc atgatccgct tcgtcgtcgc cgaccttcag cgcgcaacac
    12481 cggatgaaag cggggtttcg gcaagaggcg acaacgagcc gctgtaagcg gcggaagaat
    12541 gtagccacgg gcgccagccc gtggaataga cgtaaattaa aatcggaagc cccggagggg
    12601 cgaaagaatc gcaagcccgc ctgaaacgta gcttgaggtt ctttcgccct tccggggctt
    12661 caaaatcttt atcgtgacct gtccccacgg cttacgccgt gggctacatt ctgccgcccc
    12721 cttgcggggg ctggttgtcg ctcggctctc acgcgcccgt ctcttccctg aagcctttgt
    12781 agacgccggg gcgcgcgggg ttgaagccgt agacggcgcg ctggcggatg cggctgaaga
    12841 cgagcaggtt gaagaagtgc atcgcgccga ggacgagcaa gaccagcccg atcttccacg
    12901 acagggcttc gatgctggcg cgcgcgtcca cgacggccca gccgattttc aaggcgagcg
    12961 tgacgtagcc gaaattaatc aggtagaagc cgacgacgag caggtggttg accgagtcgg
    13021 ccagagactc gttgccgtgg aacacgtcaa cgaggaagac gcggccgttc ttgtggagcg
    13081 tctgcgcgac ccagaccgtc agcccgacgc tgaccatcag gtagacgagg tatgtgacga
    13141 cgatggtgtt catgatccgg cgcctccatg tgtctttcaa acatccggcg cttgcgggcc
    13201 gacttgggcc ttccgcgtca gttatctgtc ctcccgtaac tcaagagccg tgccgggcgc
    13261 gccgcgccct tcgtcccaac tcgaacccgg cgtttcctgc gtgtgcctgt ggcacaatgc
    13321 cgattcatgc tagagtcttc gcattccgcc acgcgccgag gaggagaccg aatgagccgc
    13381 gagccacttg accaccccac agaccctacg ggccgccccg agccgccgcc cgcgcagccg
    13441 cacgaccccg cgacccccga cccgtcgaag ccggagccgc tgccgttgcc gcccgatgcg
    13501 ccgacgccgc gcgcgcccgt cgaggagcct ggccccgccc cactcccggc cggcgacccg
    13561 cccccgcttg agccgacgcg tctcgcctga aaactttctt ccccgcccgc ggggccatct
    13621 cttataatcg acgaagcgat ggaaacgacg acgacgaccg ccggaacttc ctcgccccgc
    13681 cgggcagaag aagacgcgcg cgaatcggac tcacgagtcc gaccgcaagg ccgcgcgtct
    13741 cgcctgcaag acatcgcccg cgcgttagag cagcgcccct tcacgccaca cacactcttc
    13801 cgcggcggcc acgcgcagac cctgcgcggc gcgctgcgct ggccccgccg cctgcggatg
    13861 ctgcgcgagt acgcgggcgc cgagcggcgc ctcttcgaca tcgagccggg cgtgcgtctg
    13921 ctcgcgcact gccgctggca gacgcgaaag accgatcacc cgacactcgt gctcgtccac
    13981 ggcctcgaag ggtcgagccg ctcggtctac atgctgggca cgggacgcaa ggccttccgc
    14041 gcgggcttca acgtcgtccg cttaaacgtc cgcaactgcg gcggcaccga aagcctgtcg
    14101 cccacgcttt accacagcgg gatgagcggc gacctcctgc gcgtcttcgc cgaactcttc
    14161 gagcacgacc gcctgccgga aatcttcctc gccggcttct cgctcggcgg caacctcgtc
    14221 ctcaaactcg cgggcgaact cggcgccgac gcgcccggcg cgctcgccgg ggtggcggcc
    14281 gtctcgccct cgctcgacct ctcggcctcc gccgacgcca tcgagcgccc gcagaaccgc
    14341 ttctatcagt ggagcttcat ccgctcgctg cgtcgcacac tccgccgcaa gcacaaactc
    14401 ttcccgcacc tctacgacct gagtcgcctg cgcgagatgc gcaccatccg gcagttcgac
    14461 gacttctata cggccccgca tggcggcttc cgcgactccg ccgactacta cgcgcgctcc
    14521 agcgccctcg cgctcgccgc gcggatacgc gtcccgacac tcatcatcca cgcgcacgac
    14581 gaccccttca tcccctatgc cccactccgc aaccgggcta tcagcgacaa cccggacatc
    14641 ctgctgctcg acccgccgag gggcggacac gtcgccttcg tcgggagcgc gcggcgcggc
    14701 gaagaccgct tctgggccga gaaccgcgtc gtccaattct gtacgctgct ctcccgcggg
    14761 ggccgttgag acttgcatcc gcgagcgcag gagcaataag cttgggcgaa tttttcataa
    14821 agacttgaag gagactccag acgcgccggg cagccggcgt cactattctg aaggatgatt
    14881 actgctctca aactcttcgc cgtcgttatc attgtcctcg tcaacggctt cttcgtcgcg
    14941 gccgagttcg ccttcgtcgg ggtgcggcgg ggccgcatcg aggccgaggc cgaggcgggc
    15001 aacaagcgtg cgcgccgcct gctcggcatc ctcggcaacc tcaacgccta tctgtcggcc
    15061 gcgcagctcg gcatcacgct cgcctcgctc gccctcgcca tcctcggaga gccgaccatc
    15121 gcctcgctca tcgagggacc gatagccagg acactcgaag gccgcctgag cgccgagatg
    15181 gtcgagacca tcacgcacgg cctcgctttc gccatcgcct actcgatcct gacctcactg
    15241 cacatcacgg tcggcgagca ggcgcccaag ctgttcgggc tcgagcgcgc cgagcgcgtc
    15301 gccatgttct cggcgctccc gatggaagtc ttctacaaaa ccttccgctg gcccatccac
    15361 gccctcgact gggcgagcgc ggcgatggtt cgtctcttcg gcctccacgc gtcgagcgcg
    15421 cacgcctcga tctacacgca ggaggagatt cgccagatca tcgagatgtc gcacaagggc
    15481 ggccacgtgg aggccgacga gcagcgactc ctcaaccgcg tcctcgactt caccgacgcc
    15541 gaggtgcgcg aggcgatggt gccgcgcacg gccgtcgccg ccctgagcgt caccgccacg
    15601 ctcgaagagg ccgaagccgc cttctgcgag ggcggctact cgcgtctgcc cgtctaccgc
    15661 gagcgtctgg acgaggtggt cggcgtcctc tttatgaaag acctgatgcc gtgcctgcgc
    15721 cgcctcgacc ccgccgagtt cgacatggag cgcgcgctcc acccgccgct gttcgtgccg
    15781 gcggcggcgc ggctcgggtc ggtgctcgcg cagatgcagt cggcgcagac gcacctcgcc
    15841 tgcgtcgtcg atgagcacgg cggcctcgaa ggcatcgtca cgctcgaaga cctgctcgaa
    15901 gagatcgtcg gcgagatcaa cgacgagttc gacgaggagt cgcgctcaca gattaccgag
    15961 gagtccggcg gcacatacct cctcgacggc atgctggccg tgcgcgacgc caaccgccgc
    16021 ttccagctca agctcgacga ggaggccggc tacaccaccg tcgccggctt cctgctggcg
    16081 aagtccgggc gtctcctccg acccggcgag gtggtcgagc acgacggcgc gcgcttccag
    16141 gtcgagacgg ttgaccgccg acgcatccgc cgcatccgct acacccccgc cgccgccgcc
    16201 cccggccccg aggacgaaga ggccgcctcc cgcacgcacg cctgagccgc cgcccgcgcc
    16261 cccgacacat gtccgacaac gccaccaccg aactcatcat cgtcctcgcc gtgatgctcc
    16321 tgctcttcat cttcgggctg gcggccgtcg tcatcttctg gcgcgtgtgg cggagggaac
    16381 aaaaaagtag agttgatgga gacagcgccg acgagccgcg gagcggcggc agaatgtagc
    16441 cacgggcgcc agcccgtgga attgacgtta cggacaatga ccaagccccg gaggggcgaa
    16501 agaaatttat aggcgtcaac ctctaatttc tttcgcccct ccggggcttg gttcgtattg
    16561 cgccgccgtc acccacggct cacgccgtgg gctacattct gccgccgctc cgcggctcgt
    16621 tgatgctttc gtcgatgctt tcgtcgtgac tggtcttact tgtggggctt tgccgccttc
    16681 tccagcttct tcaaaatctt gccggccgtg ttgcgcgtga tgagcgggac gcgctcatcg
    16741 gtggagaggt ctttgaggag cggggcgagt tgttggggat cggagacgcc gccgcgctcg
    16801 acgaggcggc cgaggtattc gagcagcttc ggtgtttcga gtggctggtt gtcgcgcgcg
    16861 acgttgatgt cgaagacgaa catcgcctga tcggcggcgc ggcgatactc ggaggcgagg
    16921 gccgaggcgt cggggtcgcg gctgtagttg aactctgtcg tgcggttgcg cgcgccgcgc
    16981 gccatggaga gacgcacggt tccgaggtgc gggaactgct tgtcggcctg atagctcgct
    17041 tcggagtcga ggaaacgcag ggcttcccaa tggccgcgga tgcgccgcag cgccacggcc
    17101 gaaagctcca gcggctcttc gatggcctcc tcgctcgact tgcgctcgaa ggtgagcatg
    17161 ccgcgccccg ccgcgtcgtg cacgatgcgg atgcggctga cgacgaattt ctcgttctcg
    17221 aactcgtagc ggtagcgcgc ttcttcgacc tcggccgcgc cggctgcaac ggtgtcgccg
    17281 gcggtctttg cgggcgacgc cgacggctgt tgcgcgctgc ggccgtcgcc cgccgtcgcc
    17341 gacggccgct cggtcgtctt cttctgcgcg cgcgacgcgg aggccgcgcc cgaaactacg
    17401 agcgcggccg tcagcatcag ccctgtcatt gccctgcgct ttattttcac tcgaacctga
    17461 ccgagacgag cttcgagatg cccacctcct ccatcgtcac cccgtagagc gcccgcgccg
    17521 tctccatcgt ccgcttgttg tgcgtgatga cgatgaactg cgtctcctcg gacatctgcg
    17581 cgatcttcgc ggtgaagcgt ccgatgttgg cttcgtcgag cggggcgtcc acctcgtc
//
LOCUS       DASVES010000044         3536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1316344, whole genome shotgun sequence.
ACCESSION   DASVES010000044 DASVES010000000
VERSION     DASVES010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316344"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF240_01800"
     CDS             <1..475
                     /locus_tag="VF240_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7174005.1"
                     /translation="WKLNDAFRGLWLVLIGLFLWDAARSVYGGRRRGARTVADAMGAP
                     VAVEPDTLVSHFIDHVLPMHRQPAVAVSHDGRLHGILTLEDLKRLPRESWHRTRVSEV
                     MRPVAPQLFVDPSAPLDRAMKLMASNGAGALAVIDPSGQLVGFLLRGQIKRRAKT"
     gene            complement(481..1335)
                     /locus_tag="VF240_01805"
     CDS             complement(481..1335)
                     /locus_tag="VF240_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX7174006.1"
                     /translation="MCFSDECDSSDDVTGRRSFIAGAAATVASFAVSHGQAAVTPQVK
                     QPETRVLDDARVQHGRVMFKHNGVETFGGFLARPKVEGVYPAVLVIAGNKISEEYIPN
                     TCAALALAGFVGLAPDIFHPLPDDTPSTHEAYKKFVGNHTELDLLDDIHAGMSHLRAQ
                     RFVRPGGMGVVGFCMGGRLAMLLGARSREIDAVVPFHPAPMKPEEIARLKTPVQIHHG
                     TADRAVAVDESVKTEKMLKAQGTPVELHIYEGADHGFLAYTRPYYKPDLAKLAWARAA
                     AFLQKHLK"
     gene            1669..2625
                     /locus_tag="VF240_01810"
                     /pseudo
     CDS             1669..2625
                     /locus_tag="VF240_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
     gene            2714..>3536
                     /gene="cdaA"
                     /locus_tag="VF240_01815"
     CDS             2714..>3536
                     /gene="cdaA"
                     /locus_tag="VF240_01815"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX7174007.1"
                     /translation="MLLLQTYDHYIERLREPRSVVDVVVVFILVYALLKAIRGTRAAP
                     MAAGIAAIALIYWLAARYDLATLEFILRPAPLYIGVAVVVLFQNEIRQALISFGNRFR
                     MPFMLRHRGQFGEGAYDEIVLAATTLASTKTGALIVIERSVSLANVIEGGVKMDAELS
                     YDLLVTIFNPATPLHDGAVVVRRHRVAAAACFLPLTLNPLLSKDLGTRHRAAIGITED
                     TDAVAVVVSEETGLVSFVQGGQIRRGLDGTRLRAAILDAMEPGSRDAETEGERQTE"
BASE COUNT          603 a         1188 c         1108 g          637 t
ORIGIN      
        1 ctggaaactc aacgacgcct tccgcggcct gtggctcgtc ctcatcggac tcttcctgtg
       61 ggacgcggcg cgctcggtct acggcggccg tcgccgcggc gcgcgcaccg tggccgacgc
      121 gatgggcgcg cccgtcgccg tcgagcctga cacgctcgtc agccacttca tcgatcacgt
      181 cctgcccatg caccgtcagc ccgccgtcgc tgtttcgcac gacggccggc tccacggcat
      241 cctcacgctc gaagacctga agcgtctgcc acgcgagagc tggcatcgca cgcgcgtcag
      301 tgaagtgatg cgccccgtcg ccccgcaact cttcgtagac ccttcggcgc ccctcgaccg
      361 cgccatgaag ctcatggcca gcaacggcgc cggcgcgctg gccgtcatcg acccttcagg
      421 tcaactcgtc ggcttcctcc tccgcggcca gatcaagcgc cgcgccaaaa cctagcgcgt
      481 ctatttcaaa tgcttttgca ggaaggcggc ggcgcgtgcc cacgcgagct tcgcgaggtc
      541 gggcttgtag tacgggcgcg tgtaggcgag gaagccgtgg tcggcgcctt cgtagatgtg
      601 tagctcgacg ggcgtgccct gcgccttgag catcttctcc gtcttaaccg actcatcaac
      661 cgctaccgcg cggtcggccg tcccgtgatg aatctgcacg ggcgtcttga ggcgcgcgat
      721 ctcttccggc ttcatcggcg cgggatgaaa agggacgacc gcgtctatct cgcgcgagcg
      781 cgcgccgagg agcatcgcga ggcgcccgcc catgcagaag ccgacgacgc ccatcccgcc
      841 ggggcggaca aaccgttgtg cgcgcaggtg gctcatgccg gcgtgtatgt cgtcgagcag
      901 gtcgagttcg gtgtgattgc cgacgaactt cttgtacgcc tcgtgggtgc tgggcgtgtc
      961 gtcgggcagc gggtgaaaga tgtcgggggc gaggccgacg aagccggcga gcgccagcgc
     1021 cgcgcaggtg ttcgggatgt actcctccga aatcttgttg ccggcgatga cgagcacggc
     1081 cgggtagacg ccttccacct tcgggcgcgc aaggaagccg ccgaaggtct ccacgccgtt
     1141 gtgcttgaac atcacccgcc cgtgctggac gcgcgcgtcg tcgagcacgc gcgtctccgg
     1201 ctgtttgacc tgcggcgtca cggccgcctg cccgtgcgag acggcgaagc tcgcgaccgt
     1261 ggccgccgcg cccgcgatga aagaccgcct gcccgtgaca tcgtcgctgc tgtcgcactc
     1321 gtcgctaaag cacattgtcg ttagcctcct gaagtttgtg tggcgcggaa agtttaagga
     1381 tactgctcaa agaagaaaac agctcagagg gctgtctggt tacaaaaaag tagtgacctc
     1441 attcggggat gttctctgcg cgtcctttgc gtcctttgcg actctgcagt gagtcgctgc
     1501 tgaagcacac tcaccgcaga gtcgcaaagg acgcaaagga cgcgcagaga gttgatatta
     1561 ggacacgacc cgtttatgcg gattgttgtc ttgcgccgtt ttttgcagca taatcggcgc
     1621 ggaggcgttt agcttcaatc accgggagga cataagtgaa cgctcgcagt gctcagtgca
     1681 ctcgttgcca atggtctaat cacaatcctg aagttcatcg gcgccatgat gacgggctcc
     1741 tcgggcatga tggccgagtc gctccactca ctggccgaca cgacgaatca ggttttcctc
     1801 ctgttgggtt tgaggttcta caagaggccc gcgtcggagt agcacccgtt cggctacggc
     1861 aaggagcgtt tcttctggtc gttcatcgcg gcgattttca tcttcggggt cggcgccacc
     1921 tacgccgtct acgagggcat ccagaagctg acgcacccgc acccgccgga gaagctggag
     1981 tgggcctact ggattctcgg catctcgttc gtgctcgaag ccggctccat tgcgctggcc
     2041 gtctatcagg aggtcaagga ggcgcgccac gagggggtga gcttcggcga gtacctgcgc
     2101 gagtcgaaag acccgaccgc caagaccgtg atctttgagg attcggccgc gctcctcggc
     2161 atcgtcatcg ccttcatcgg catctacctg acagaccatc aggtggggcc ggtctggcat
     2221 gagggcgaga cgccgggcgc ctactgggac ggcgtcgcct caatcgccat cggcgtggtg
     2281 ctcgccatcg tcgccttcgt ccttgcgcgc ttctcgcgcg ggctgctgat cggcgaggcg
     2341 gccacgaaga agtcgctcga aaagattcgg gccgccatcc gctcgcaccc gaacgtcgtc
     2401 gaggtgatcg agctgctgac gatgcacctc gcgccgaagc agattctcgt gaacgcgcac
     2461 gtcaacttcc gcaacgagct ggcgaccgac gacatcgaga agaccatcaa cgaggtcgag
     2521 gcgctaatca agcaggccga gccgatggtt gacatgatct ttctggagac ggcgcgcgaa
     2581 agcgaccccg gcgccgacaa gccgatcccg cagcacatcg gctgacccga ccgaacgggc
     2641 gcaggcgaac tggtaaaaga cccccacggc gtatgacacg cgagttgatt aagagagacg
     2701 agggacagcg gcagtgcttc tgctacagac atacgaccac tacatcgagc gcctgcgcga
     2761 gccgcgcagc gtggtggacg tggtcgttgt cttcatcctc gtctatgccc ttctgaaagc
     2821 catccgcggc acgcgcgccg caccgatggc cgccggcatc gcggccatcg ccctcatcta
     2881 ctggctcgcc gcgcgctacg atctcgccac cctcgaattc atcctccggc ccgcgccgct
     2941 ctacatcggc gtggccgtcg tcgtgctctt ccagaacgaa atccgtcagg cgctcatctc
     3001 tttcggcaac cgcttccgca tgccgttcat gctccgccac cgcgggcagt tcggtgaggg
     3061 cgcctacgac gagatcgtcc tcgccgccac cacgctcgcc tccacgaaga ccggcgcgct
     3121 catcgtcatc gagcgctcgg tgagcctcgc caacgtcatc gagggcggcg tgaagatgga
     3181 cgccgaactc tcctacgacc ttctggtgac catcttcaac ccggcgacgc ccttgcacga
     3241 cggcgcggtc gtcgtccgcc gccaccgcgt cgccgccgcg gcctgcttcc tgccgctcac
     3301 cttgaacccg ctgctctcga aagacctcgg cacgcgccac cgcgcggcca tcggcatcac
     3361 ggaggacacg gacgccgtcg cggtcgtcgt ctccgaagag acggggctcg tctccttcgt
     3421 gcagggcggg cagatacgcc gtggcttgga cggcacgcgc ctgcgcgccg ccatcctcga
     3481 cgcgatggag cccggctcgc gcgacgccga gacggaaggc gagaggcaaa ccgagg
//
LOCUS       DASVES010000045        16336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_346651, whole genome shotgun sequence.
ACCESSION   DASVES010000045 DASVES010000000
VERSION     DASVES010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346651"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..798)
                     /gene="tsaE"
                     /locus_tag="VF240_01820"
     CDS             complement(337..798)
                     /gene="tsaE"
                     /locus_tag="VF240_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7174008.1"
                     /translation="MSAAERETVPTGVWESHSSEDTFAVGASIGERLSGGEILLLHGA
                     LGAGKTVFVKGVAAGLGMDVEEVTSPSFTLVNCYDDGRLTLYHIDLYRLDEGSAAAHA
                     VDLDELLSDERAVLAVEWAERMGRYPLPDGVWHVRIEGDGDDPRRISVAKD"
     gene            complement(795..2468)
                     /locus_tag="VF240_01825"
     CDS             complement(795..2468)
                     /locus_tag="VF240_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX7174009.1"
                     /translation="MRKIITAAEMREIDRLTTERFGLPSLLLMESAAGAAARAVAGYF
                     SEGISGKTALILCGRGNNGGDGAALARILWTMGAKADIVLFGRVENSAGDARTNFEIV
                     RHLASFEAGSGERPSGVSFVECETVAAWEEIASRRRGYDLLVDALLGTGLTRPVEGVF
                     RKVIDHLALVREARRHAGETRPLIVSLDLPSGLDADSATPPGEAVRADLTVTFTAPKP
                     ANVLPPASHFNGRLLTSDIGSPPSLLEATPSKLFLAEPADARIWLERTRYAPASYKNT
                     HGHVLVVAGSRQYTGAPALCANAAARSGAGLVTVATPVSAHAAVTAHLLPEVIAAALP
                     ETGQGAVGDESLAALAPLVERATVVAVGPGLTANAEETRRFVRELVERRTTPIVIDAD
                     ALNSLAPWPADLRGSRELPLVLTPHEGEMRRLLGTKDRDAVSDRVHAAGEFASANEVV
                     LVLKGARVLVAAPDGRVFVNPTGNAGLGRGGSGDTLTGVVAGFLAQSYATLKDRADAF
                     EATVAAVYVAGLAGDIAAREGGMRTLLASDVRDCLGEAIRSLDAEGETP"
     gene            complement(2823..3470)
                     /locus_tag="VF240_01830"
     CDS             complement(2823..3470)
                     /locus_tag="VF240_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7174010.1"
                     /translation="MAFFAICLASTATVRADTINIVGNATGSQATATMTCSFNAQTNT
                     FTFTIVNTSPFDARITGIGFDLPPVGNASASGLNGFTGNVVFQPAGVDFEFGEGNLGN
                     VPQFNDVVLDFGFTTGNSGNFAGGNPNDGLPPGIDVGEEATFVVSGAAFAGFTEQQIC
                     EAMYVRFQRVGANGEGSDVGRPADPMPEPTTMLLLGTGLAGIAAKVRRRRNAAQE"
     gene            complement(3692..5629)
                     /gene="murJ"
                     /locus_tag="VF240_01835"
     CDS             complement(3692..5629)
                     /gene="murJ"
                     /locus_tag="VF240_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7174011.1"
                     /translation="MKRIPDADPADKKDSPADTQPDGDVGACLDRTSPAQATDGELVG
                     NVIGEELDKESGTAAGEAETPAAATTAGLAGSAKEDAETSSPQRPAPQHSVARSAGIV
                     SLAVMGSRILGLVREVVFAALFGAGFLKDAFDIAFKIPNLLRDLFAEGALSVAFVKTF
                     TDYTEKRTEQEAWRLASLVMNALAVVLSVITLIGVFFSPQIVAVLAGGFSPEKAALAV
                     TLTRIMFPFLLLVALAAVAMGVLNTKGRFGIPASASTMFNVGSIAGGLLFAYFLSGGG
                     WASPLDPNAIPAPAAQWAIIGMAIGTLIGGGLQFLIQLPSLWKVGFRFRPVLSFTDPG
                     VRQVMRLMGPAVIGTAAVQINVMINAFYASGINGGVSWLNYAFRLMQLPIGIFGVAIG
                     TATLPAISRFAARNDIPSFRSTLSSSLGLVFLLTIPSACGLVALGRPIIALIYQRGSF
                     SALTTDMVALALACYAVGLAGYAAIKVLSPAFYALDDARTPMIISLLSIGVNAVGSYF
                     LRSWFGAFGVTPETPSGYAHAGLALSTSLIALCNFFALVYFMRRRIHRLEGRRIVSSF
                     VRIAAASAALSAASYLTYLWLHAQFGGRNLSARLAEAFVPIAAGGVVFLLAARLLRVR
                     ELDQAMQAFAGRFLRRPTKLT"
     gene            complement(5626..7008)
                     /locus_tag="VF240_01840"
     CDS             complement(5626..7008)
                     /locus_tag="VF240_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7174012.1"
                     /translation="MPDILLVEDKDSLRRVLRLTLEHAGYSVAEAADAREAAAEIARA
                     PFRLVLTDLRMPHGSGMDVLRAARAADPEVPVVVMTAYGSIDEAVQAMKDGAHDFLQK
                     PVDSNHLLLLIGRALETARLRTENVLLREEWARRYGFPRIVGESDAIKRAVAETQRVA
                     TTDTTVLLLGESGTGKELFARAVHHLSPRRAGPFVALNCAAIPETLIESELFGHERGA
                     FTGASERRAGKFELASGGTVFLDEVGELPTAAQGKLLRVIEEKVVDRIGGRAPVPVDI
                     RVVAATNRDLQRAAEEGSFRRDLYFRLAVFPVRIPPLRERDDDAVLLAHHFAAQLGRE
                     LRKREATLSDEAVEAIRRHPWPGNVRELENAVERACILADAPALAPADLGLVPRAARA
                     TRDDLAAVFDLSGTLSEAAERAARQVERRKIAEALAAHGGNKTRAAETLGVSYKTLLT
                     KIKDYALDPQ"
     gene            complement(7236..9068)
                     /locus_tag="VF240_01845"
     CDS             complement(7236..9068)
                     /locus_tag="VF240_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HEX7174013.1"
                     /translation="MKHTTLKPHATHRRGERGAALVMTLLISTLLLAAGGSLIFSTMM
                     SATGAIDSTPEQQAYYAAESGLQGALNVLRGNVAASPALSAGQTMTFTRALSLADSND
                     TLGGDTSADSRLSRWLQYNYPTNAGLAARDRIVLGNAATYSPLTGMAYSVVITDPYDQ
                     DRMVFSTTGTFTAPAVSGGVTASTTNGNTRLTVTDATGANRVTLEFVPKNETTLASAY
                     TATASDLGIVSVVEAVGATDFDDTDFFTSSPKIRIRVNQTAPWSSTDFLEGTLTGSIS
                     RNAAGALTNSIVIRYPTQPSVLTDGGTYTLTAAASGNPAVYRVNLNGTDTTLAATVTV
                     AEPKYVLVTSTGYGPRGARKQLQMMVSRSAIMFDPPAAITAAGGAGITIDLGSSNASD
                     FDGLGSRPAVAVSTTNYGAATTAVTNSNTQGSGTQVTPATVGQLGDDVRQPSFLDTPD
                     AARAFLETLRKQAMPTGEDTSADRYFTTMPAPAQMGATGTPKLTFIDNYGGAAVTLGT
                     GNQGSGLLIVTGDLDTDGGTDFSGIILVLGSGRMTRSGGGGGTIGGSIIVACFNPNNP
                     DADTFCNPTYDASGGGNSTTEFDPDAIANALKFAGRGVLGVVEN"
     gene            complement(9095..9736)
                     /locus_tag="VF240_01850"
     CDS             complement(9095..9736)
                     /locus_tag="VF240_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7174014.1"
                     /translation="MKRAQQQQQGRGREAGFSLIELIIAMGLTLTVMSMASILLAEAF
                     GIRSREHVRVEAIADAQRGLNILSREVANSGFGLTGNGIVAADSNANSIRVRANLNSF
                     GGAGAQNATTDADEDLIFSIIDNGVEKLIYRHDVNSGVPPSPLADRVDSLEIEYLDED
                     DAEVAPDLAKKVRLTIGVLLPTVGNPSSPGYQPASRTQVMSEVSLRNGDLTGY"
     gene            complement(9778..10248)
                     /locus_tag="VF240_01855"
     CDS             complement(9778..10248)
                     /locus_tag="VF240_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174015.1"
                     /translation="MSATVAQGARRGRGGEGGFTLIEICISLLIMMIAGLGVVTVFAF
                     AVNYNQGAGDRASAYAVAQQKVEIVRSTAYADLTAALAAAHTGAVERGSSGTDLRRFD
                     VTMTITTNELVIDGTPRRKTITVTVTPRGLSDKHWSSGAVTLVTQRASNDMGPN"
     gene            complement(10274..11398)
                     /locus_tag="VF240_01860"
     CDS             complement(10274..11398)
                     /locus_tag="VF240_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7174016.1"
                     /translation="MLEVKSDGADTARKSRRSEGGFTAIEVIIVVAVVTIVAALAVMT
                     MARARDNVRLVGAARELAAHIEKARLDSIRRHELGGQALVTLTSANTYSVLMDFAGSG
                     ATTTRNFTFPDGIKITNDPLPPAIGFNWRGRTTADARITLTNSSEEEEVVSVTINGEV
                     SVGREVIVLASPDSTAIAPGSGINSAIPTSTTSGSTTTTTTGGTSSTTSGTGSTSSTS
                     TTTGDATTTTTGSTSGTTTGTTTGATTGTTTGTTTGTTTGTTTGATTGTTTGATTGTT
                     TGTTTGITPTCSITAASSSMNVKKNHSATIDVTVTGTTTITASSDNVSVTVTLLQTSG
                     NVHTYTLSSINNYKGYTAKVTFVAGCDPSVKANVDVNVVP"
     gene            complement(11695..13095)
                     /locus_tag="VF240_01865"
     CDS             complement(11695..13095)
                     /locus_tag="VF240_01865"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX7174017.1"
                     /translation="MGRSQIKKLVLAIVMLAAPLSGAAPASAQVGSASSSQQQQRPRT
                     VVVPAPVALATPEALPTPTPPPFEGVSAQAAARPTIFRHGILVEDDDGDLIIEQGADQ
                     TYNPASAVKLATALQALRTLGADHRFPTAVWSTGAFDRTTGTITGDLIISGRDPSFHY
                     QHAVALARELNHIGVRTVTGDLVIAPKFTMNFGASALRSGERLYDTLDSTRRPAAAVR
                     AWSDERVALKDAEALVTTPSVAVMGAVYVDSVPAGAQLLLTHRSSRLEDVLKVLLCYS
                     NNFMAERLGDTLGGPAGLERFLQTELQIPPGEVRLSSTSGLGVNRLSPRNMLRIYREL
                     LEELDEHGLKASDIMPVAGIDPGTLSKRFAHHPSRGSIIAKTGTLTRTDGGASALVGQ
                     LRARDGKTLYFVIFNQRGSVARFRGAQDQLLYNLQLAHGGPALFPYVPQALAMRLSDT
                     EYQTAKNSEEYEPESN"
     gene            13422..14084
                     /locus_tag="VF240_01870"
     CDS             13422..14084
                     /locus_tag="VF240_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174018.1"
                     /translation="MPREPKPVTVEAIRATHRERAPEIRLRLSEFRAVWERGTDARLW
                     EEMVYCIFTAGASARMGLRSVEAVRPLLDDGAHDDLAGALSGVHRYPRARSGYVVTTR
                     DFLRRDCSLRLRERLESFSDPVERRDWLARERGIKGLGYKEASHFLRNVGLSGYAILD
                     KHILRCLAEVGVIDSPQPPATRARYLAAEERLRAFARQLRIDFDELDLVLWSMKTGEI
                     LK"
     gene            14200..15792
                     /locus_tag="VF240_01875"
     CDS             14200..15792
                     /locus_tag="VF240_01875"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX7174019.1"
                     /translation="MRLNRQISAGGFIALAVCAAVLVAGEWAGAAAARAGDKNLRRAE
                     KALRAGEFEVAEKLFREMVTKDKGDAGARLGLSHALLKQRKHQDAYDHAARVVATDPL
                     SYRAHSLLGSALLGAGDFRLSVEEFRTALGLKDDDAMAIAGLAMVDFYENRLQASLNG
                     LRRATSISPDEPDFQFNLGQIAARSERYAEAANAYERFLRIAPLTDADRRARIRGLID
                     FLRYLGTQNELMQTGGPARSVVPFELVNNRPIVRVRINNSKETLRFVVDTGAGMCVIA
                     AGAAERLGLRAVARGGMARAIGGRFEIVYGFLQSMHVGDTRVSNVPVYIRHFERQQES
                     VDGYLGLSLISKYLTTVDYGAREMLMLRDDALPKYDPAQPPAGAEVPIRVTSSGFWSG
                     EIRIGEIERAANFIIDTGATTSVVSQAFADREAMSRFAQPGHIRVYGAAGVTENVQTL
                     RLPRVHLGPHAHEGVSAAVLDMEPINETAGFEQTGIVGGNLLRHYRVTFDFARSVVRL
                     EPLVSAPKEERTTAAPVVTSQP"
     gene            15789..16268
                     /locus_tag="VF240_01880"
     CDS             15789..16268
                     /locus_tag="VF240_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7174020.1"
                     /translation="MKQSIYLETSVVGAYLDNGEPFRRDLTIRWWEHEMSEYRAVISP
                     LVTRELERVPEPHRTGYLKLAGALERVDLTDEATILAEGYVARGIFHRKYLGDALHVA
                     VASFHKIDYLVTWNFGHLANVRRQARIRLFNTAAGFFVPMIVTPEFLVSELTEKGDK"
BASE COUNT         2606 a         5061 c         5855 g         2814 t
ORIGIN      
        1 gacctcctcg actgtgcgcg tttcctcgac accggcgctc gtcaaatcct cgcgcattgg
       61 tcggatgaga aattcaggat agggcatctg ttataaatcc tccttcaaaa gttctttcgt
      121 ttccgctaag cattctaaca ccgagcggcc cgtcgcgccg cctcgcgccg gtcgtgtcct
      181 cacatcaact ctctgcgccg cctcggcgtc ctccgcggct cggcggtgaa tactcgcttc
      241 ggcacactca ccgcagagtc gcaaagggcg cagaggccgc gcagagaaaa accccaaatg
      301 agggcgccac cggcacgccg tgccagccag acgtgctcag tccttcgcga cggaaatccg
      361 ccgcgggtcg tcgccatcgc cttcgatgcg gacgtgccac acgccgtcgg gcagcgggta
      421 gcgtcccatc cgctcggccc actcgacggc gaggacggcg cgctcgtccg acagcagttc
      481 gtcgagatcg acggcgtgcg cggccgccga gccttcgtcg agtcggtaga ggtcgatgtg
      541 atagagcgtg agccgtccgt cgtcgtagca gttgacgagc gtgaaggagg ggctggtcac
      601 ttcctccacg tccatcccca atccggcggc gacgcccttc acgaagacgg tcttgcccgc
      661 gccgagcgcg ccgtggagca gcagaatctc cccgcccgag aggcgctcgc cgatgctggc
      721 gccgacggca aaggtgtctt ccgagctgtg ggattcccac acgcccgtcg gcaccgtctc
      781 gcgctcggcc gcgctcacgg cgtctctcct tccgcgtcga ggcttcggat cgcctcgccc
      841 aaacaatcgc gcacgtctga ggcgagcagc gttcgcattc cgccctcgcg cgcggcgatg
      901 tccccggcca acccggcgac gtagacggcc gcgaccgttg cctcgaaagc gtcggccctg
      961 tctttcaacg tggcgtaaga ctgcgcgagg aagccggcga cgacgccggt cagcgtgtcg
     1021 cccgagccgc cgcgaccgag gccggcgttg cccgtggggt tgacgaagac gcgcccgtcc
     1081 ggcgcggcga ccagtacgcg cgcgcccttt aaaacgagga cgacttcgtt ggccgacgcg
     1141 aactccccgg ccgcgtgtac cctgtccgac accgcgtcgc ggtcttttgt cccgagcagg
     1201 cgacgcatct cgccttcgtg cggcgtgagc acgaggggta actcgcgaga gccgcgcaag
     1261 tcggcgggcc atggcgcgag cgagttgaga gcgtcggcgt caatcacgat gggcgtcgtg
     1321 cgacgttcga ccaactcgcg cacgaagcgg cgcgtttctt ccgcgttcgc ggtcaggccg
     1381 gggccgacgg cgacgaccgt cgcgcgctcc acgagcggcg cgagggccgc gagcgattca
     1441 tcgcccacgg ctccttgccc cgtctctgga agcgcggccg cgatgacctc cggcagaagg
     1501 tgggcggtga cggcggcgtg cgccgagacg ggcgtcgcta cggtcacgag tcccgcgccc
     1561 gagcgcgccg cggcgttggc gcacagggcg ggtgcgcccg tgtactggcg cgagccggcg
     1621 acgacgagga cgtgcccgtg cgtgttcttg taggaggcgg gcgcgtagcg cgtccgttcg
     1681 agccagatgc gtgcgtcggc cggctcggca aggaaaagtt ttgaaggcgt ggcttcgaga
     1741 agggaaggcg gcgagccgat gtcggaggtt aacagtcgcc cgttgaagtg ggaggcgggc
     1801 gggagcacgt tggccggctt gggggcggtg aaggtgacgg tgaggtcggc gcggacggcc
     1861 tcgccgggcg gcgtcgccga atccgcgtcg aggcccgatg gcaggtcgag ggaaacgatc
     1921 agcgggcgcg tctcgccggc atgccgccgc gcctcgcgga cgagcgcgag gtggtcgatc
     1981 acctttcgga agacgccttc gacggggcgc gtcaggccag tcccgagcaa cgcatcgacg
     2041 aggaggtcat atccgcgccg gcgactcgcg atctcctccc acgcggccac cgtctcgcat
     2101 tccacgaacg aaacgccgga cgggcgctca cccgaacccg cctcgaaact cgccaggtgt
     2161 cgcacgatct cgaagttcgt ccgcgcgtcg ccggcggagt tttccacgcg tccgaacagg
     2221 acgatgtccg ccttcgcccc catcgtccag aggatgcgcg cgagggcggc gccgtcgccg
     2281 ccgttgttgc cgcgcccgca aaggatgagc gcggtcttgc ctgatattcc ttcagaaaag
     2341 tatccggcaa cggcccgcgc cgccgccccg gccgcagact ccatgagcag cagagacggg
     2401 aggccgaaac gctccgtcgt caggcggtcg atctcgcgca tctccgccgc cgtgatgatt
     2461 tttcgcacgg acgggatgat atgaggacgc ggcggcgcgg gtcaaacggt ttcctggggg
     2521 attcgacagg caggcaggga ttcttcggcc gacagaaaca aaaagacgac gcaaaccaag
     2581 cgcactattc cgcgcctgac tgccgccgtc gcctgccgcc cgccgattcg acccttgttt
     2641 tccggtattt acaggaaatg tttaattaag ttgtgtctcc agaaaaattc tcgagtgggc
     2701 gatttttttt aagtgggagt gaaaaaaaag tgaaaggccg gaaggtcttt gctgacctcc
     2761 cggcccttta gtgtgaaaaa ccacagaaac cgacactttg gcgggcacgt gtgccgcttg
     2821 ggctactcct gggccgcgtt gcgacgcctg cgcaccttgg cggcgatccc tgccaggccc
     2881 gtgccgagca gcagcatggt ggtcggctcg ggcatcggat cggccggccg acccacgtcg
     2941 ctgccctcgc cgttcgcgcc gacgcgctgg aatcgcacgt acatggcttc gcagatctgc
     3001 tgctcggtga agccggcgaa ggccgccccg ctcaccacga acgtcgcctc ctcacccacg
     3061 tcgatgcccg gaggcagccc gtcgttcggg ttcccgcccg cgaagttccc cgagttgcca
     3121 gtggtgaacc cgaagtcgag caccacgtcg ttgaactgcg ggacgttgcc caagttgcct
     3181 tcgccgaatt cgaagtcgac gcccgcgggc tggaagacga cgttgcctgt gaagccattg
     3241 aggcccgagg cgctggcgtt gcccaccggc ggcaggtcga agccgatgcc ggtgatgcgg
     3301 gcgtcgaagg gcgacgtgtt aacgatggta aaggtgaagg tgttggtctg cgcgttgaac
     3361 gagcaggtca tggtggccgt cgcctggctg cccgtggcgt tgccgacgat gttgatcgtg
     3421 tcagcccgga ccgtcgcggt cgaggcgagg cagatggcaa agaacgccat tattgcaaaa
     3481 gttagttttc ttgaatgggg catcatcggt ctccttagtt ttccttgatc gtcgaatttc
     3541 gtgccgctgc ccggctccgt aggattcgtc agacattctt tctgatctgt ctatcccaaa
     3601 agactcgcat tagcttgagt tttccaggat tgacgaaaaa aaacgtgaag gacgtcgaac
     3661 ggatatgaac ggacagagtg agtgtgggcg atcaagtgag cttggtcggg cggcgcagga
     3721 aacggcccgc gaaagcctgc atcgcctggt cgagttcccg cacgcgcagc agtctggccg
     3781 cgagcaggaa gaccacccca ccggcggcga tggggacgaa ggcttcggcg aggcgggcgc
     3841 tcagattgcg cccgccgaat tgcgcgtgga gccacaggta tgtcaggtag ctcgccgccg
     3901 agagggcggc cgaggcggcc gcgatgcgga cgaaagagga gacgatgcgg cgcccttcga
     3961 ggcggtggat gcggcggcgc atgaagtaga cgagggcgaa gaagttgcag agcgcgatga
     4021 gggaggtgga gagcgccagt ccggcgtggg cgtacccgct cggcgtctct ggcgtgacgc
     4081 cgaaggcgcc gaaccaggag cgcaggaagt agctgccgac ggcgttgacg ccgatggaga
     4141 ggaggctgat gatcatcggc gtgcgcgcgt cgtcgagcgc gtagaaggcc ggcgagagca
     4201 ccttgatggc ggcgtagccg gcaaggccca ccgcgtagca ggccagcgcc agcgcgacca
     4261 tgtcggtcgt gagcgcgctg aaggagccgc gctggtagat gagggcgatg atggggcggc
     4321 cgagagcgac gaggccgcag gccgagggga tcgtcagcag gaagacgagg ccgagcgagg
     4381 aggagagggt ggaacggaag gaggggatgt cgttgcgggc ggcgaagcgt gagatggcgg
     4441 ggagcgtggc cgtaccgatg gcgacgccga agatgccgat gggcagttgc atcaggcgga
     4501 aggcgtagtt gagccaggag acgccgccgt tgatgccgga ggcgtagaag gcgttaatca
     4561 tcacgttgat ctgcacggcg gccgtgccga tgacggccgg ccccatcaga cgcatcacct
     4621 gccgcacgcc cgggtcggta aaactcagca cggggcggaa acggaagccg actttccaga
     4681 gtgaaggcaa ctggatgagg aactggaggc cgccgccgat gagcgtccct atggccatcc
     4741 caatgatggc ccactgcgcg gcgggcgctg ggatagcgtt cgggtcgagc gggctggccc
     4801 agccgccgcc gctgaggaag taggcgaaca aaagtccgcc ggcaatggag ccgacgttga
     4861 acatcgtcga ggccgacgcc ggaatgccga agcgcccttt agtgttgagc acgcccatcg
     4921 cgacggcggc gagggcgacg agcaggagga aggggaacat gatgcgcgtc agtgtcacgg
     4981 cgagcgcggc cttctcgggt gagaagccgc cggcgagcac ggcgacgatc tgcggcgaga
     5041 agaagacgcc gatgagcgtg atgacgctca agacgacggc gagtgcgttc atcacgagac
     5101 tggcgagacg ccacgcctct tgttccgtgc gcttctcggt gtagtcggtg aaggtcttga
     5161 cgaaggcgac ggagagcgcg ccctcggcga agaggtcgcg caggaggttc ggtattttga
     5221 aggcgatgtc gaaggcgtct ttgaggaagc ccgcgccgaa gagtgcggcg aagacgacct
     5281 cacgcaccag cccgaggatg cgcgaaccca tcacggcgag gctgacgatg ccggccgagc
     5341 gcgccacact gtgctgcggc gcggggcgtt gaggcgacga ggtttcagcg tcctctttag
     5401 cagagccggc aagcccggcg gttgtcgccg ctgctggcgt ctccgcttcg ccggcggccg
     5461 tgccgctctc cttgtcgagt tcttcgccga tgacattccc gacgagttcg ccgtccgtcg
     5521 cctgtgcggg cgacgtgcgg tcgaggcacg cgcccacgtc gccgtcgggc tgagtgtcgg
     5581 ccggtgaatc ttttttgtcg gcgggatcgg cgtcgggaat gcgcttcatt gtgggtcgag
     5641 tgcgtagtct ttgatcttgg tcaggagcgt tttgtaactg acgcccaatg tctcggcggc
     5701 gcgcgtcttg ttgccgccgt gcgcggcgag cgcctcggcg atcttgcggc gctcaacctg
     5761 acgggcggcg cgctcggccg cctcggagag tgtgccggag aggtcgaaga cggcggcgag
     5821 gtcgtcgcgg gtcgcgcgcg cggcgcgggg cacgaggccg aggtcggcgg gcgcgagagc
     5881 gggcgcgtcg gcgaggatgc aggcgcgctc gacggcgttc tcaagctcgc gcacgttgcc
     5941 gggccacggg tgacgacgga tggcctcgac cgcttcgtcc gagagcgtcg cctcgcgctt
     6001 acgcagctcg cgcccgagtt gggcggcgaa gtggtgtgcg agaagcacgg cgtcgtcgtc
     6061 gcgctcgcgc agagggggga tgcggacggg gaagacggcg aggcggaagt agaggtcgcg
     6121 gcggaaggag ccttcctcgg ccgcccgttg caagtcgcgg ttcgtcgcgg cgacgacgcg
     6181 gatgtccacg gggacggggg cgcgcccgcc gatgcggtca acgaccttct cctcgatgac
     6241 gcgcaaaagt ttcccctgcg cggccgtggg cagctcgccg acttcgtcga gaaataccgt
     6301 gccgccggag gccaactcga acttgccggc gcggcgctcg gaggcgccgg tgaaggctcc
     6361 gcgctcgtgg ccgaacaact ccgactcgat gagcgtctcg gggatggccg cgcagttgag
     6421 ggcgacgaag gggccggcgc ggcgcgggct gagatggtgg acggcgcgcg cgaaaagctc
     6481 cttgcccgtg ccggactcgc cgagcagaag cacggtcgtg tcggtcgtgg ccacgcgctg
     6541 cgtctcggcg acggcgcgct tgatcgcatc cgactcgccg acgatgcgcg ggaagccgta
     6601 gcggcgcgcc cactcctcgc gcagcaaaac gttttcggta cgcagccgcg ccgtttccag
     6661 cgcgcggccg atgaggagca ggaggtggtt cgagtccacg ggcttctgga ggaagtcgtg
     6721 tgcgccgtcc ttcatggcct gcacggcctc gtcgatggag ccgtaggcgg tcatcacgac
     6781 gacgggcact tcggggtcgg ccgcgcgcgc ggcgcgcagc acgtccatgc cggagccgtg
     6841 cggcatacgc aggtcggtga ggacgaggcg gaagggggcg cgggcgatct cggcggcggc
     6901 ctctcgggcg tcggccgcct cggccacact gtagccggcg tgctccagcg tcaggcgcag
     6961 cacgcgccgg aggctgtcct tgtcctcgac gagaaggatg tcaggcataa acgaagcaga
     7021 ggtcagaggt cagaggttag aggtcagtga aagaacatgc cggctactat cactgacctc
     7081 tgacctctaa cctctgacct ctgctcttcc gtggcggatg gtagtgtcgc gcgcgggggg
     7141 tgtcaagcag gtgtccgcaa agaaaagagg cgctgccgtt aacgtcaacg gcagcgcctc
     7201 ttttctttgc gggcttgaag agtgtgtgcg cggccttagt tctcgaccac gccgaggacg
     7261 ccgcggccgg cgaacttgag cgcgttggcg atggcgtcgg ggtcgaactc ggtggtgctg
     7321 ttgccgccgc cgctggcgtc gtaggtaggg ttgcagaacg tgtcggcgtc ggggttgttg
     7381 gggttgaagc aggcgacgat gatcgagccg ccgatagtgc cgccgccgcc gccgctgcgc
     7441 gtcatccttc ctgagccgag cacgaggatg atgccgctaa aatccgtccc gccgtccgtg
     7501 tcaaggtcgc cggtgacgat cagcaggccg ctgccttgat tccccgtccc gagtgtcacg
     7561 gccgcgccgc cgtagttgtc gataaaggtc agcttgggag tgccggtcgc gcccatctgg
     7621 gcgggcgccg gcatggtggt gaagtagcgg tctgcagacg tgtcttcgcc cgtgggcatg
     7681 gcctgcttcc tcaacgtctc gaggaaggcg cgcgcggcgt cgggcgtgtc gaggaaggat
     7741 ggctgccgca cgtcgtcgcc gagttgcccg accgtcgcgg gagtgacctg cgtcccgctg
     7801 ccctgagtgt tgctgttcgt gacagcggtc gtggccgctc cgtaattggt tgtggaaacg
     7861 gcgacggccg ggcgcgaccc gaggccgtcg aagtcagacg cgttcgagct gccgaggtcg
     7921 atggtgatac cggcgccgcc ggccgcggtg atggccgccg gcgggtcgaa catgatggcc
     7981 gagcggctga ccatcatctg caactgcttg cgggcgccgc gcgggccgta gcccgtggag
     8041 gtgacgagca cgtacttcgg ctccgcgacc gtgacggtgg ccgccagcgt cgtgtcagtc
     8101 ccattcaggt tcactcggta gacggccggg ttgccggagg cggcggcggt gagcgtgtag
     8161 gtgccgccgt cagtgaggac ggagggctgg gtcggatagc ggatgacgat ggagttggtg
     8221 agcgccccgg cggcgttacg ggagatggag ccggtcaggg tgccttcgag gaagtcggtg
     8281 ctgctccagg gggcggtctg gttgacgcgg attctgattt tgggggagga ggtgaagaag
     8341 tcagtgtcgt cgaagtcggt ggcgccgacg gcttcgacga cactgacgat gccgaggtcg
     8401 gacgccgtcg ccgtgtaggc cgacgcgagc gtggtctcgt tcttggggac gaattcgagc
     8461 gtcacccgat tggcgccggt ggcgtccgtc accgtgagcc tcgtgttgcc gttggtcgtc
     8521 gaggcggtca cgccgccgct gacggccggc gccgtgaacg tccccgtggt tgagaagacc
     8581 atgcggtcct ggtcgtaggg gtcggtgatg acgacgctgt aggccatgcc ggtgagcggc
     8641 gagtaggtcg ccgcgttacc gaggacgatg cggtcgcggg cggcgaggcc ggcgttcgtc
     8701 gggtagttgt actggagcca gcgcgagagg cgcgagtccg cggaggtgtc gccgcccagc
     8761 gtgtcgttcg agtcggcaag gctgagggcg cgggtgaagg tcatcgtctg cccggcggac
     8821 agggcggggc tggcggcgac gttgccgcgc aggacattga gcgccccctg aagtcccgac
     8881 tcggcggcgt agtaggcctg ctgctcgggc gtcgagtcga tggcgcccgt ggcggacatc
     8941 atggtgctaa agatgagcga gccgccagcc gccagcagca gcgtcgagat caggagcgtc
     9001 atgaccaggg ccgcgccgcg ctcgccgcgg cggtgggtgg cgtgtggctt cagggtggtg
     9061 tgtttcatat cggcctcgtc gattccgttg atgattaata gccggtcaag tctccgttgc
     9121 ggagcgacac ttcgctcatg acctgcgtgc gcgaggccgg ctggtagccg ggggagctgg
     9181 ggttgccgac cgtggggagc aggacgccga tggtgaggcg cacctttttc gccaggtcgg
     9241 gggcgacttc cgcgtcgtcc tcatcgaggt attcgatttc cagcgaatca acgcggtcgg
     9301 ccagcggcga cggcgggacg ccggagttca cgtcgtggcg gtagatcagt ttctcgaccc
     9361 cgttgtcgat gatggagaag atcaggtctt cgtcggcgtc ggtggtggcg ttctgcgcgc
     9421 cggccccgcc gaagctgttg aggttggcgc ggacgcggat gctattggcg ttggagtcgg
     9481 cggcgacgat gccgttgccg gtgaggccga agcccgagtt ggccacctcg cgcgagagga
     9541 tgttgaggcc gcgctgggcg tcggcgatgg cctccacgcg gacgtgctcg cgggagcgga
     9601 tgccgaaggc ctcggccagc aggatgctgg ccatgctcat caccgtgagc gtgaggccca
     9661 tggcgatgat cagctcgatt aaagagaagc cggcctcgcg gcctcgcccc tgctgctgct
     9721 gttgcgcccg tttcatgtgg cctcctgatt cctgcggggt gggtgggctc gcgactccta
     9781 gttcggcccc atgtcgttcg aggcgcgctg ggtgacgagc gtcacggcgc cgctgctcca
     9841 atgcttatcc gacaaccctc gcggggtgac ggtgacggtg atcgtcttgc ggcgcggcgt
     9901 gccgtcgatg acgagttcgt tggtcgtgat ggtcatcgtc acgtcgaagc ggcggaggtc
     9961 tgtccctgag gagccgcgct cgacggcgcc ggtgtgcgcc gcggcgaggg ctgccgtcag
    10021 gtcggcgtag gccgtgctgc ggacgatttc gaccttctgc tgcgcgacgg cgtaggcgct
    10081 cgcccggtcg cccgccccct ggttatagtt gacggcgaag gcgaacaccg tgacgacgcc
    10141 gaggccggcg atcatcatga tgaggagcga gatgcagatc tcgatgagtg tgaatccgcc
    10201 ctccccaccg cgcccacggc gcgcgccctg cgctactgtt gcagacatcg atcctgccct
    10261 cctcagagtg tgcttacggg acgacgttga catcgacatt cgccttgacg ctcgggtcgc
    10321 agccggcgac gaatgtgacc ttggccgtgt aacccttgta gttgttgatg gaactgagtg
    10381 tgtacgtatg gacgttgccg gaagtctgga ggagcgtgac ggtgacgctc acattgtcgc
    10441 tgctggcggt gatggtcgtc gtaccagtca cggtgacgtc aatcgtcgcc gagtggttct
    10501 tcttaacatt catcgacgac gaggcggccg tgatcgagca ggtgggcgtt atgcctgtcg
    10561 tggtgcccgt cgtcgtcccc gtggtggcgc ctgtcgtggt gcccgtggta gcgccggtcg
    10621 tcgtgcccgt cgtcgtgccg gtggtcgtcc cggtcgtcgt gcctgtggtg gcgccggtcg
    10681 tcgtgccggt cgtcgtgccc gatgtcgagc cggtggttgt ggtcgtcgcg tcgccggtgg
    10741 tcgtcgatgt gctggatgtc gagccggtgc cgctcgtcgt cgagctggtg ccgcccgtcg
    10801 tcgttgtcgt cgtgctgccg ctcgtcgtgc tggtggggat ggccgaattt atcccggagc
    10861 cgggagcgat ggccgtggag tcgggagacg cgagcacgat gacttcgcgc ccgaccgaga
    10921 cctcgccgtt gatggtcaca ctgacgactt cctcctcctc cgagctgttg gtcagggtga
    10981 tgcgcgcgtc ggcggtggtg cggccgcgcc agttgaagcc gatggccggc gggagtgggt
    11041 cgttggtgat cttgatgccg tcagggaagg tgaagttgcg cgtggtggtg gcgccgctgc
    11101 cggcgaagtc catcagcacg ctgtaggtgt tcgcgctggt gagcgtcacc agggcctggc
    11161 cgcccagctc gtggcggcgg atggagtcga ggcgggcttt ctcgatgtgg gccgccagct
    11221 cgcgcgccgc cccgacgagt cgcacgttgt cgcgcgcgcg cgccatcgtc attacggcca
    11281 acgcggcgac gatggtgacg accgcgacga cgataattac ctcgatggcc gtgaagccgc
    11341 cttccgagcg ccgactcttc cgcgcggtgt cggccccgtc acttttaact tcaagcatta
    11401 tccgcctcca gccagcgtct tcgattgcgt gtgcccggcg cggcagcgcg ggcgctatct
    11461 cacgtccggc acagtttgca aagacggtgc caccgcggaa gagtcgatta tccggccaat
    11521 tcagcgtcgg ggagcggcgg ggcgttgatc gaggcgttac ggatggtgac aggcgtgggt
    11581 agccgagttc atagagggga aggcagaggg gaaggcaaaa gtaaaaaggc aaaaggcaaa
    11641 agtgaagagg cgacgtgcgt tctcactttt gccttttgcc tttttacttt tgccttaatt
    11701 cgactcgggt tcgtactcct cggagttctt cgccgtctgg tattccgtat cggagaggcg
    11761 catggcgagg gcttggggca cgtaagggaa tagcgcgggg ccgccgtggg cgagttgtag
    11821 gttgtagagg agttgatcct gtgcgccgcg gaagcgcgcg acgctgccgc gctggttgaa
    11881 gatgacgaag tagagcgtct tgccgtcgcg cgcgcgcagt tggccgacga gggcgctggc
    11941 gccgccgtcg gtgcgcgtga gcgtgccggt cttggcgatg atgctgccgc gcgagggatg
    12001 gtgggcgaat cgctttgaga gcgtgccggg gtcgatgccg gcgaccggca tgatgtccga
    12061 agccttcagt ccgtgctcgt caagctcttc gagcagttcg cgataaatcc tgagcatgtt
    12121 gcgcggcgaa agacggttga cgccgaggcc gctcgtcgaa gacagacgca cctcgccggg
    12181 cggtatctga agctctgtct ggaggaagcg ttcgagaccg gccgggccgc cgagcgtgtc
    12241 gccgaggcgt tcggccatga agttattcga gtagcagagc agcaccttga gcacgtcttc
    12301 gagccggctc gaacggtgcg tcaacagaag ctgcgcgccc gccggcaccg agtccacata
    12361 gacggcgccc atcacggcca cgctcggcgt agtcacaagg gcctccgcat ctttgagcgc
    12421 cacgcgctcg tcgctccaag cgcggacggc ggccgcgggc cggcgcgtcg agtcgagggt
    12481 gtcgtaaagc cgctcgcccg agcgcagggc cgacgcgccg aagttcatcg tgaacttcgg
    12541 cgcgatgacg agatcgccgg tgacggtgcg gacgccgatg tggttcagct cgcgcgccag
    12601 cgccacggcg tgctggtaat ggaaagacgg gtcgcggccc gagatgatga ggtcgccggt
    12661 gatcgtgccg gtcgtgcggt cgaaggcgcc ggtagaccag acggcggtcg ggaagcggtg
    12721 gtcggcgccg agcgtgcgga gcgcctggag cgccgtcgcc agcttaacgg ccgacgccgg
    12781 gttgtaggtc tgatccgcac cctgctcgat gatgaggtcg ccgtcgtcgt cttcgacgag
    12841 gatgccgtgc ctgaagattg tcgggcgggc ggcggcctgc gccgagacgc cctcgaaggg
    12901 cgggggcgtc ggcgtcggca gtgcctcggg cgtggccagc gcgacgggcg cgggcacgac
    12961 gaccgtgcgc ggccgctgct gctgttgcga cgacgatgcg ctgccgacct gtgcgctcgc
    13021 tggagccgcg ccactgagcg gcgcggccag catgacgatg gcgagaacta attttttgat
    13081 ttgtgacctg cccatgtgct cgacctcggc tcgaaggtgc tccgctcgaa cggcgcgcat
    13141 catagcacac cgcacgacga cgctcggttc caaaatccgc gcgcgagcgt cggagggaga
    13201 gccaactcct tagatgcacc cgtgcatcgt accggatgta ttcatccggt aagcgtccta
    13261 aacggccaaa gcaactttca ttccgacgag tcgtcccgcg gggccgttga aaaaactgtt
    13321 cctttaatgt gtcggaggcg tgcgttgaca acgccgaaag cccgctccta aaatccgacg
    13381 cgtcggaagc gagtcgccgc cacgcccctt tctattcagc catgccccga gaaccgaagc
    13441 ccgtcaccgt cgaggccatc cgcgccacgc accgcgagcg tgcgccggag atacgcctgc
    13501 gtctctcgga gtttcgcgcc gtctgggagc gggggacgga cgcgcgcctg tgggaggaga
    13561 tggtctactg catcttcacg gccggcgctt cggcgcgcat gggactgcgc tcggtcgagg
    13621 ccgtcaggcc gctgctcgac gacggcgcac acgacgacct cgccggcgca ctctcgggag
    13681 tccaccgcta cccgcgcgcg cgctccggct acgtcgtcac gacgcgcgac ttcctgcggc
    13741 gcgactgctc cttgcgtctg cgtgagcggc tggaaagttt ttccgacccc gtggagcggc
    13801 gcgactggct ggcgcgcgag cgcggcatca aaggtctcgg ctacaaagag gcgagccact
    13861 tcctgcgaaa cgtcggactc agcggctacg ccatcctcga caaacacatc ctgcgctgcc
    13921 tcgccgaagt tggcgtcatc gattcgccgc agccgcccgc gacgcgcgcc cgatacctcg
    13981 ccgccgagga gcgcctgcgc gccttcgccc ggcagctccg catcgacttc gacgaactcg
    14041 acctcgtgct ctggtctatg aagacggggg agatactgaa atgaaggatg agggcggagg
    14101 gatgagggat gaacaagatt gagtgtgtgg ctcatcgacg ctaacactca gtccggttca
    14161 tccctcatcc ttcatccttc atcccttcgg agttttgcca tgagactcaa tcgacagatt
    14221 tcggccggcg ggttcatcgc gctggcggtg tgtgcggcgg tgctcgtcgc gggcgagtgg
    14281 gcgggggcgg cggcggcccg ggcaggggac aagaatcttc ggagggctga gaaggcgctg
    14341 cgcgcgggcg agttcgaagt cgccgagaag ttgttccgcg agatggtgac gaaggacaag
    14401 ggcgacgcgg gcgcgcgcct cggcctgagc cacgcgctcc tcaagcagcg caagcatcag
    14461 gacgcctacg accacgcggc gcgtgtcgtg gccactgacc cgctgtcgta ccgcgcacac
    14521 tccctgctcg gctcggcgct gctcggcgcg ggcgacttcc gtctctcggt cgaggagttt
    14581 cgcacggccc tcgggcttaa ggacgacgac gcgatggcca tcgccggcct cgcgatggtt
    14641 gacttctacg agaaccgact tcaggcgagc ctcaacggcc tgcgccgcgc cacctccatc
    14701 agccccgacg agcctgactt ccagttcaac ctcgggcaga ttgccgcccg ctccgaacgc
    14761 tacgccgagg ccgccaacgc ctacgagcgg tttctgcgta tcgccccgct caccgacgcc
    14821 gaccggcgcg cgcgcatccg cggcctcatc gacttcctgc gctacctcgg cacgcagaac
    14881 gagctgatgc agaccggcgg ccccgcgcgc agcgtcgtcc ccttcgagct ggtcaacaac
    14941 cgccccatcg tgcgtgtgag gatcaacaac tcgaaggaga cgctgcgctt cgtcgtggac
    15001 acgggcgcgg ggatgtgcgt catcgcggcg ggcgcggccg aacgcctcgg tctgcgcgcg
    15061 gtcgcccgcg gcggcatggc ccgcgccatc ggcgggcgct tcgagatcgt ctacggcttc
    15121 ctccagtcga tgcacgtcgg cgacacgcgc gtctcgaacg tgccggtcta catccgccac
    15181 ttcgagcgtc agcaggagtc ggtggacggc taccttggcc tgtcgctcat ctcgaagtac
    15241 ctgacgaccg tggactacgg cgcgcgggag atgttgatgc tgcgcgacga cgccttgccc
    15301 aagtacgacc cggcgcagcc gcccgccggc gcggaggttc cgatccgcgt cacttcgagc
    15361 ggcttctgga gcggcgagat caggatcggc gagatagaaa gggcagccaa cttcatcatc
    15421 gacacgggcg ccaccacgtc cgtcgtctcg caggcgttcg ccgaccgtga ggcgatgtcg
    15481 cgcttcgcgc agcccggaca catccgcgtc tacggcgcgg ccggcgtcac cgagaacgtc
    15541 cagacactac ggcttccgcg cgtccacctc gggccgcacg ctcacgaggg cgtgtcggcg
    15601 gcggtgctcg acatggagcc gatcaacgag accgccgggt tcgagcagac cggcatcgtc
    15661 ggcggcaacc tcctgcgcca ctaccgcgtc accttcgact tcgcgcgctc cgtggtgcgt
    15721 ctggagccgc tcgtctccgc gccgaaggag gagcgcacga cggcggcgcc cgtcgtgacg
    15781 agtcagccat gaagcagagc atctatctgg agacctcggt cgtcggcgcc tacctcgata
    15841 acggcgagcc gttccggcgc gacctcacca tccgctggtg ggagcacgag atgtcggagt
    15901 atcgcgccgt catctcgccg ctcgtcacga gggagctgga gcgcgtcccg gagccgcacc
    15961 gcaccgggta tctgaagctc gcgggcgcgc tcgaacgggt cgatctcact gacgaggcga
    16021 ccattctcgc cgaaggctat gtcgcgcgcg gcatcttcca ccgcaagtat ctcggcgacg
    16081 ccctgcacgt cgccgtcgcc tccttccaca aaatcgatta tctggtgacg tggaacttcg
    16141 gccacctcgc caacgtgcgc cgccaggcgc gcatccgtct cttcaacacc gccgccggat
    16201 tcttcgtccc gatgatcgtc acgccggagt tcttagtttc ggagttaact gaaaaaggtg
    16261 acaagtgaca ggtgacaagt gacaagatta aattcttgct ttaacttgtc actcgtcact
    16321 tgtcacttgt cactgt
//
LOCUS       DASVES010000046         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_148122, whole genome shotgun sequence.
ACCESSION   DASVES010000046 DASVES010000000
VERSION     DASVES010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148122"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..924
                     /locus_tag="VF240_01885"
     CDS             127..924
                     /locus_tag="VF240_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX7174021.1"
                     /translation="MSIVTLLTDFGTADYFVGAMKGAILSADPSAQLVDITHDIPPHD
                     IEAAAFTLLAAFESFPAGTVHLAVVDPGVGSERRPIAIESREHLFVGPDNGVFGHVYE
                     RLKNFRVFHLTNDDYFRRDVSTTFHGRDIFAPVAGALSRGVPADALGPPVTDAVRLAL
                     AAPRRLDDGTLEASVIHIDRFGNLITNITPRDLPDEAIHRGARLRIGERDIKSFRHFF
                     AEDDAAAGEPFAIWGSAGLLEIAVYRDSAARLLDARRGQKIIVNGKS"
     gene            1076..2227
                     /locus_tag="VF240_01890"
     CDS             1076..2227
                     /locus_tag="VF240_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX7174022.1"
                     /translation="MDKSEEKTGGAPKGPAGLEGVVAAQSSIGDVDGVQGRLIYQGYD
                     IHDLARNSTFEETIYLLWHGRLPKRDELDDLRRQFDANQTLPAEVLDIMRRFPPGAEP
                     MDSLRTAVSALAFYDTGARDLSREGALRTATRLTAQFPVVVAAIERLRRGEELVAPKP
                     GLGLAAGFLHMLKGEEPNEREARIFDVCLVLHADHELNASTFTGRVVAGTLADMYGAV
                     TAAIAALSGPLHGGANTNVMKMLLEIGSVERAEEWMMDALANKKKIMGFGHRVYRTED
                     PRSVHLRDFARELGEARGDTRWIDILDRLREVMFREKKLYPNVDYYSGAAYHMMGIPL
                     DLFTPIFAVSRISGWTGHILEQYANNRLIRPRAEYTGARDLKNVPVDER"
BASE COUNT          453 a          868 c          676 g          401 t
ORIGIN      
        1 cagctcaggc tccgcacctc cggcagataa gaacggagtg ataagtgatg agtgataagt
       61 gataagaagg gggaagcagg gctttttctt atcactcatc actcatcact tatcaccttc
      121 tctctcatgt caatcgtcac cctgctcacc gacttcggga cggccgacta tttcgtcggg
      181 gcgatgaagg gggcgatact ctcggccgac ccgtcggcgc agcttgtcga tatcacgcac
      241 gacatcccac cgcacgacat cgaggccgcg gccttcacgc tgctcgccgc cttcgagagt
      301 tttcccgcgg gcacggttca cctcgcggtc gtcgatcccg gcgtcggctc cgagcggcgc
      361 cccatcgcca ttgaatcacg cgaacacctc ttcgtcgggc cggacaacgg cgtgttcgga
      421 cacgtctacg agcgcctgaa aaatttccgc gtcttccatc tcaccaacga cgactacttc
      481 cgccgcgacg tgagcaccac cttccacggc cgcgacatct tcgcgcccgt cgccggcgca
      541 ctctcgcgcg gcgtccccgc cgacgcgctc ggcccgcccg tcacggacgc cgtccgcctc
      601 gcgctcgccg ccccgcgccg cctcgacgac ggcacgctcg aagcctccgt catccacatc
      661 gaccgcttcg gcaatctcat caccaacatc accccgcgag acctgcccga cgaggccatc
      721 caccgcggcg cgcgtctccg tataggcgaa cgcgacatca aatccttccg acacttcttc
      781 gccgaagacg acgcggccgc cggcgagccg ttcgccatct ggggcagcgc cggcctcctc
      841 gaaatcgccg tctaccgcga ctccgccgcc cgcctcctcg acgcgcgccg cgggcagaaa
      901 atcatcgtga atggtaaatc gtaaatcgtg aatcgtggtc gagtgagagc ctggcttgtg
      961 cgataaagcg taagtcgcgc cgcggtcaac ccaatcgatt tacgatttac gatttaccat
     1021 tcacggtttt ttttgctatt gtcgggccac atcacaactt gagggactga acacaatgga
     1081 taagtcagaa gagaagacgg gcggcgcccc gaaaggcccg gccggactgg agggcgtggt
     1141 cgccgcgcag tcttccatcg gcgacgttga cggcgtgcag ggtcgtctca tctatcaggg
     1201 ctacgacatc cacgacctcg cccgcaactc cacctttgaa gagaccatct acctgctctg
     1261 gcacggccga cttccgaagc gcgacgagct ggacgacctg cgccgccagt tcgacgccaa
     1321 ccagacgctc cccgccgagg tgctcgacat catgcgccgc ttcccgcccg gcgccgagcc
     1381 gatggactcc ctgcgcacgg ccgtctcggc gctggccttc tacgacacgg gcgcgcgcga
     1441 cctctcgcgc gagggcgcgc tccgcaccgc cacgcgtctg acggcgcagt ttcccgtcgt
     1501 cgtcgccgcc atcgagcgcc tgcgccgggg cgaggaactg gtcgcgccga agccgggtct
     1561 cggcctcgcc gcgggcttcc tccacatgct caagggcgag gagccgaacg aacgcgaggc
     1621 gcgcatcttc gacgtgtgcc tcgtcctcca cgccgaccac gaattgaacg cctcgacctt
     1681 tacgggccgc gtcgtcgccg gcacgctcgc cgacatgtac ggcgcggtga cggccgccat
     1741 cgccgccctc tccggcccgc tccacggcgg cgccaacacc aacgtgatga agatgctcct
     1801 cgagatcggg agcgtggagc gcgccgaaga gtggatgatg gacgcgctcg ccaataagaa
     1861 gaagatcatg ggcttcggcc accgcgtcta ccgcacggaa gacccgcgct ccgtccacct
     1921 gcgcgacttc gcgcgcgaac tcggcgaggc gcgcggcgac acgcgctgga tcgacatcct
     1981 cgaccgcctg cgcgaagtga tgttccgcga gaagaagctc tacccgaacg tcgattacta
     2041 ctcgggcgcg gcctatcaca tgatgggcat ccccctcgac ctcttcacgc ccatcttcgc
     2101 cgtcagccgc atctccggct ggacgggcca catcctcgaa cagtacgcca acaaccgcct
     2161 catccgcccc cgcgccgaat acaccggcgc ccgcgacctc aagaacgtgc cagttgacga
     2221 gcggtagcca gcgacacacc aagaaaagaa cgtgccgccg gacgcgcttt tacatgagcg
     2281 caactccggc ggcacatttg tttgtctaac gctctaatta actcgcgccg acgagcagca
     2341 ttcaatgcta caccgcaagg agaaatataa agggacgcta tgacagggtc aggttata
//
LOCUS       DASVES010000047         6573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_731262, whole genome shotgun sequence.
ACCESSION   DASVES010000047 DASVES010000000
VERSION     DASVES010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731262"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1665)
                     /locus_tag="VF240_01895"
     CDS             complement(121..1665)
                     /locus_tag="VF240_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEX7174023.1"
                     /translation="MMDDAPRLKLVELVSRHGRGLAGEPRRCEALLRDYCGEHRREIS
                     ALTSAAEEHAAAELLTPQRGTPRAALLGRLARRLCDHVAITEEAATWAVNSWALALGV
                     VSDDELKTLESPTVARAEADAATATPAPDAAPRVATNTPHVVTAASSALTPIVVAADG
                     SGDYVSIGEALENAAAGARLLVRPGVYEEGVIIDEHVEIVGDGPVEKIVIRSAAASCL
                     RMRGAQARVAGLTLQGVAGSGEGVFAVDIEQGRLLLEDCRITSETLSCVAVHGRSTEP
                     LIRRCRISDGADSGLYFFDGARGSVEDCEIHGHSNVGVAITAGADPAVRRSKIYGGAN
                     AGVASWGEGRGVLEECEIYGNRLAGVGVSEGARLSARACRIYEGDNSGVFVHGGGDAT
                     LEACDIHGHRAAEVAVKTLGQLTALRCQIHEGRGAGVFIREGGQALIQECGVTLNAGA
                     GVSIGDGSLAAVLGCQVKDNGEAGIRVAAGGAARVNESDLSGNRSGAWDVEEGAFVEG
                     GDNAEE"
     gene            complement(1662..2501)
                     /locus_tag="VF240_01900"
     CDS             complement(1662..2501)
                     /locus_tag="VF240_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX7174024.1"
                     /translation="MAYAHHIEPGRKKIKLRDFDPDDTGGLKREEAEEKTDKLIEELV
                     ELQELLYAASRKSVLVVLQGRDTSGKDGAIKSVTGPLNSQGCDVSSFKAPTEQELAHD
                     FLWRVHAQTPAAGHIKIFNRSHYEDVLIARVHKLVPEKTWRARYEHINNFERLLADAS
                     TIILKFYLHISREEQEKRLRKREQDSTKFWKLSVGDWKEREHWDDYTAAYEDALSLCG
                     TKDAPWFVVPSNKKWFRNLAIAEALARALRPHRARWLKHLEEVGESELKAIEEYRQGG
                     TTP"
     gene            complement(2504..3532)
                     /locus_tag="VF240_01905"
     CDS             complement(2504..3532)
                     /locus_tag="VF240_01905"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX7174025.1"
                     /translation="MTDTQSRPEWRVIGETVPGASHLRAGIPNQDAIFQVRESGRELP
                     LVVSVSDGHGSDKCFRSDRGSRFAVRRAAQLVGEFLDERRGPLDLSGMEGELKERVPA
                     EFVRRWREAVEADLKTRPFTAEELDTLEKKDDARARRLVEDNPLIAYGATSLTVAVEE
                     SFILYLQLGDGEIITVSESGEVTKALPGDSRLFANETTSLCTPDAEKNFRSRLQPLTN
                     ERPALILLSTDGYANSFSDEAGFLKVGADLLEMMRADGLDSVNASLKGWLEEATRTGS
                     GDDTTVSVVCRMDALQKLPLTVEGVPAAPEQKETASAAVDETQAASASPKAQAANAPS
                     GAPAEGKT"
     gene            complement(3545..4315)
                     /locus_tag="VF240_01910"
     CDS             complement(3545..4315)
                     /locus_tag="VF240_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174026.1"
                     /translation="MSRRPGGELASRPLHFIWIADSSGSMEQDGKIQALNTAIREAIP
                     HMRKVAEDNPNAQVLVRAVKFSNGAQWHLSQPTPVADFAWTDLVADGETDMGKALQLV
                     AEQLKMPPMSERALPPVLVLISDGQPTDDYDAGLKALLNEPWGKKAVRIAISIGRDAN
                     DEVLQEFIGNSELKPLAANSPEALVKHIKWASTAVLKSASSPAAGADTPVGAATPPPS
                     VPIPVAATPSAQAAAAPGGAQTPADTDADNQKTIDDVW"
     gene            complement(4544..5428)
                     /locus_tag="VF240_01915"
     CDS             complement(4544..5428)
                     /locus_tag="VF240_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174027.1"
                     /translation="MSDVPRQTLRDLVARHGPGLCSDARRCEGLLRDLCGAHRREINI
                     LISALKQHVPLDLLAAQGSMPRGLLLARLARRLEEQLALTAEAARWAVDSWALALGVV
                     TDAELQDRQRQTVKAAAPPPPPTFGEDDAEKTAGEPARATPPVATQSRQPHVARPTPQ
                     TSQTSQTAPPPPATRQAGRRTSPLPPLPAPPPPQRQRAGRGPFSNWVVRSGPRSNAPS
                     QTGMAQQQQQQAPPRGASPDPHARRRRGRSLRGCTIGCLLLVLLSVALFLGVPFVVSV
                     LREEQQRRVNETPPAMSP"
     gene            complement(5442..5666)
                     /locus_tag="VF240_01920"
     CDS             complement(5442..5666)
                     /locus_tag="VF240_01920"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HEX7174028.1"
                     /translation="MFGSSLVIVAVVAGVGLALLGLLAFGGRLSKFGRLPGDVRYSGR
                     NTRVFIPVTSMLLLSIVLSVLLTLLGWLFG"
     gene            6134..>6573
                     /locus_tag="VF240_01925"
     CDS             6134..>6573
                     /locus_tag="VF240_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174029.1"
                     /translation="MKEKRTRLEILLALSVMAFAAALLLFGSRGPARRGAVGSAFVPA
                     PTSAAAAEFSPWKLASRMVTEERGEPTGRQAKVEVPGQLRHYSDTRRFLAIQVAEWKE
                     HGVATPHDYADLAALIRAGELVEVPTVGDSYVLFGVGGSADKQL"
BASE COUNT         1006 a         2237 c         2163 g         1167 t
ORIGIN      
        1 tgtccgtggt cagtagtccg ttgtcagttg catgtgtggc ccggcttgac ccttgcaata
       61 caagcgacga gttgccggac gaaaacaacg aaccactgac tactggccac ggacagcttt
      121 tcactcttcc gcgttgtcgc cgccctcgac gaacgcgcct tcttccacgt cccacgcgcc
      181 cgaacggttg ccgctcaaat cgctttcgtt gacgcgcgcc gcgccgcccg ccgcgactct
      241 aatgccggcc tcgccgttgt ctttgacctg acatccgagc acagcggcca gcgagccgtc
      301 gcctatcgag acgcccgcgc cggcgttaag cgtcacgccg cattcctgaa tcaaagcctg
      361 tccgccctcg cggataaaaa cgcccgcgcc gcggccctcg tgaatctggc agcgcagggc
      421 cgtcagttgg ccgagcgtct tcacggcgac ctcggccgcg cggtgtccgt ggatgtcgca
      481 agcctcaagc gtggcgtcgc cgcctccgtg gacgaagacg ccggagttgt cgccctcgta
      541 gattcgacag gcacgcgccg aaagcctcgc gccttcgctg acgccgacgc ccgccagccg
      601 attcccgtaa atctcacact cctccagcac cccgcgccct tcgccccacg acgcgacccc
      661 ggcgttcgcg ccgccgtaaa ttttgctgcg ccgcacggcg gggtcggccc ccgcggtgat
      721 ggcgacgccg acgttcgagt ggccgtgtat ctcacagtct tcgacgcttc cgcgcgcgcc
      781 gtcgaagaag tagaggcccg agtcggcccc gtctgaaatc cggcagcggc gaatcagcgg
      841 ctcggtggaa cgtccgtgga cggcgacgca tgaaagcgtt tctgacgtga tacgacaatc
      901 ttcgagcagg agtcgcccct gctcgatgtc cacggcgaag acgccctcgc cactccccgc
      961 gaccccttga agcgtcagcc ccgcgacgcg cgcttgagcg ccgcgcatcc gtagacaact
     1021 cgccgcggcg ctcctgatga cgatcttctc cacggggccg tcgccgacaa tctcgacgtg
     1081 ctcatcaatg atcacgccct cttcatacac gccgggtcgg acgagcaggc gcgcgccggc
     1141 cgcggcgttc tcaagcgcct ccccgatact cacgtaatcg ccgcttccgt ccgccgcgac
     1201 gacgatgggc gtgagcgctg aagacgcggc cgtgacgacg tgtggcgtgt tcgtggcgac
     1261 tcgcggcgct gcgtccggcg cgggcgtcgc cgtcgcggcg tctgcttccg cgcgggcgac
     1321 ggttggcgat tcaagagtct tcaactcgtc gtccgaaaca acgccgaggg ccagcgccca
     1381 cgagttgacc gcccacgtgg cggcctcttc ggtaatggcg acgtgatcgc acagccgccg
     1441 ggcgagacgc ccgagcagcg ccgcgcgcgg cgtgccccgt tgcggcgtca acaactcggc
     1501 cgcggcgtgt tcctcggcgg cgctcgtgag ggcggaaatc tcgcgccgat gctcgccgca
     1561 gtaatcgcgc agcaacgcct cacagcggcg cggctctccg gccagaccgc gaccgtgacg
     1621 cgacaccagc tcgaccagct tcaggcgcgg cgcgtcatcc atcatggcgt cgtgccgccc
     1681 tggcgatact cttcgatggc cttcaactcg ctctcgccca cttcctccag atgcttgagc
     1741 cagcgcgccc tgtggggccg gagcgcacgg gccagcgcct cggcgattgc cagattgcgg
     1801 aaccactttt tgttagacgg gacgacgaac cacggcgcgt ccttcgtgcc gcacaggctg
     1861 agcgcgtcct cgtaggcggc cgtgtaatcg tcccagtgct cgcgctcctt ccagtcgccg
     1921 acggacagct tccagaactt ggtcgagtcc tgttcgcgct tgcggagccg cttctcctgt
     1981 tcttcgcggc tgatgtgcag gtagaacttg aggatgatgg tggacgcgtc agcgagcaaa
     2041 cgctcgaagt tgttgatgtg ttcgtagcga gcgcgccacg tcttttcggg gacgagcttg
     2101 tggacgcgcg cgatgagcac gtcttcgtag tgcgagcggt tgaagatttt gatgtggccg
     2161 gcggcgggcg tctgtgcgtg gacgcgccag aggaagtcgt gcgccagctc ctgctcggtc
     2221 ggcgccttga acgaagacac gtcgcaaccc tgcgaattca aaggccccgt gacgctcttg
     2281 atggcgccgt ccttgccgct cgtgtcgcgg ccctgcaaca cgacgaggac gctttttcga
     2341 ctggcggcgt agagaagctc ctgcaactcg acaagctctt cgatcaactt gtcagtcttc
     2401 tcttccgcct cttcgcgctt cagaccgccc gtgtcgtcag ggtcgaaatc acgtagtttg
     2461 atcttcttgc ggcccggttc gatgtggtgt gcgtatgcca tgcttacgtt tttccttcgg
     2521 cgggcgctcc gctcggggcg ttcgcggctt gggcctttgg cgacgccgac gccgcttgcg
     2581 tctcatcgac cgcggccgac gccgtctcct tttgctcggg cgccgccggc acgccttcga
     2641 ccgtgagggg aagcttttgc agggcgtcca tgcgacagac gacggacacc gtggtgtcgt
     2701 cgccgctgcc cgtgcgggtc gcctcttcga gccatccctt gaggctcgcg ttgacggagt
     2761 cgaggccgtc ggctcgcatc atctcaagga ggtcagcgcc gactttgaga aagcccgctt
     2821 cgtcggagaa cgagttggcg tagccgtcgg tcgagagcag gatgagcgcg gggcgctcgt
     2881 tggtgagcgg ttgcaggcgt gagcgaaaat tcttctcggc gtcgggcgta cacagcgaag
     2941 tggtctcgtt ggcgaagagg cgcgagtcgc cgggcagggc tttagtgacc tcgcccgatt
     3001 cggagacggt gatgatttcg ccgtcgccga gttgcaggta gaggatgaac gactcttcga
     3061 cggccaccgt caacgaagtc gcgccgtagg cgatgagcgg gttgtcttcg acgagccggc
     3121 gcgcgcgcgc gtcgtccttc ttctccagcg tgtcgagttc ttccgctgtg aagggccgcg
     3181 tcttgagatc ggcctcgacc gcctcgcgcc aacggcggac gaactccgcg ggcacgcgtt
     3241 ccttcaactc gccctccatc ccggagaggt cgagcgggcc gcgccgctcg tcgagaaatt
     3301 cgccgacgag ttgcgcggcc cgccgaacgg cgaagcgcga gccgcggtcg ctgcggaagc
     3361 acttgtcgct cccgtgcccg tcggacacgg agacgacgag cggcagctcc cggcccgact
     3421 cacgcacctg aaagatggcg tcctgattcg gaatgccggc gcgcaggtgc gacgcgccgg
     3481 gcacggtctc gccgatgacc cgccactcgg gccgactctg tgtatcagtc atgcgctcgg
     3541 ctccttacca gacgtcgtcg atggtcttct ggttatccgc gtcggtgtcg gccggcgtct
     3601 gagcgccgcc cggcgcggcg gcggcctgtg cgctgggtgt cgcggccacg gggatgggca
     3661 cgctcggcgg gggagtggcc gcccccacgg gcgtgtccgc gccggcggcg ggcgaggagg
     3721 ccgacttgag cacggccgtc gaggcccact tgatgtgctt gacgagcgcc tcggggctgt
     3781 tggccgccag cggcttcagc tccgagttgc cgatgaactc ctgcaagact tcgtcgttgg
     3841 cgtcgcgccc gatggagatg gcgatacgca cggccttctt gccccacggt tcattcaaca
     3901 gcgccttgag tccggcgtcg tagtcgtcgg tcggctgtcc gtcggagatg agcacgagga
     3961 cgggcggcag ggcgcgctcg ctcatcggcg gcatcttgag ttgttcggcg acgagttgca
     4021 gagccttgcc catgtcggtc tcgccgtcgg ccacgaggtc agtccacgcg aagtcggcca
     4081 cgggcgtggg ctgcgacaga tgccactgcg cgccgttgga aaacttgacg gcgcgcacca
     4141 ggacctgcgc gttcgggttg tcctcggcca ccttacgcat gtgcgggatg gcctcgcgga
     4201 tggcggtgtt gagcgcctga atttttccgt cctgctccat cgagccggag ctgtcggcga
     4261 tccagatgaa gtgaagcggc ctcgatgcga gttcgccgcc gggtcgtctg ctcatagtct
     4321 tttctccttt tatcttctcg ttattgtctc gtctcaaatc tgacggaaac taactgcgcc
     4381 gtccgagtcc gtgcccgact ccgcggcgcg accttcggcc gacggccgac cgcggcggcg
     4441 acgggccgcg cgcgacgggc gcggcgcacc tatttcgtga aaggggaatc ggaaacaata
     4501 taccagaccg acggcgcgcg ccctcctctt cacgtcgtcg tcttcacggc gacatcgccg
     4561 gcggcgtctc gttgaccctg cgctgttgtt cttcgcgcag gacggagacg acgaagggca
     4621 cgcccagaaa cagcgccacc gacagcagca ccaacaggag gcagccgatg gtgcagccgc
     4681 gcaacgaccg gccgcgcctt cgccgcgcgt ggggatcggg cgacgcgccg cgcggcggag
     4741 cttgctgctg ctgctgctgg gccatgcccg tctgcgacgg agcattcgag cgcggccccg
     4801 agcgcacaac ccaattacta aacggcccgc gtcccgcgcg ctgccgctgc ggcggcggtg
     4861 gcgccggcag cggtggcaac ggcgacgttc gtctgcccgc ctgtcgtgtg gcgggcgggg
     4921 gcggcgcagt ctgtgaagtc tgtgaagtct gtggcgtcgg ccgcgcgacg tgcggctgtc
     4981 gcgactgtgt cgcgacggga ggcgttgcgc gcgcgggttc gccggcagtc ttctccgcgt
     5041 cgtcttcgcc gaatgtcgga ggcgggggcg gggcggcggc tttaaccgtt tgcctttgcc
     5101 tgtcctgcaa ctcggcgtcc gtgaccacgc cgagcgccag cgcccaggag tccacggccc
     5161 agcgcgcggc ctccgcggtc agggcgagtt gctcttccag acgccgcgcc agacgcgcca
     5221 gcagcagccc acgaggcatc gagccttgcg ccgccagcag gtcgagcggc acgtgctgct
     5281 tgagcgcgct gatgaggatg ttgatctccc gccggtgcgc cccgcacagg tcgcggagca
     5341 ggccctcgca ccgacgcgcg tcgctgcaca ggccggggcc gtgccgggcg acgaggtcgc
     5401 ggagcgtctg gcgcggcacg tcactcatgc gcgtttcagt ttcatccgaa cagccagccc
     5461 aacagcgtta gcaacacact caggacgatg gaaagcagca gcatggaagt gacggggatg
     5521 aacaccctcg tattcctgcc gctgtagcga acgtctccgg gaagacggcc aaactttgaa
     5581 agacgcccgc cgaaggccaa cagccccaac agcgccagac cgacacccgc cacgacggcg
     5641 acgatcacca gacttgatcc gaacaagata agacactcct cgtacacata gatttgcccc
     5701 ggccacggaa ggcaaccgct gagccagatg catgaagtcg ccgcttctgt ccggcgccgc
     5761 acacccgtca gccagccgcg cggcccgatt tcgtggccga ttcgcgtgaa ggccgtcagc
     5821 ctcgctcctc gtccccaatc gaagccggcg cagtagacat actgaaacgg tacaggccgc
     5881 gcccgtagtc cagacctcgg tttgtctcaa acctcggtcg atgtgagcga ttgtggcccg
     5941 gcgtctttcg caggcgcggg agggccgcgc cggggagtct tatcttgtcg ttcacacagc
     6001 ctcccttggc cggcgcgcag cgtacttttc gcccccctca gtcatctata aatgtacagg
     6061 ctcgacgagt ctgggtctcg actttaagac ccacccgccg ggccattttt ttatattcag
     6121 ggggaggcgg ccgttgaaag aaaaaaggac gcgcttagag attctgctgg ccctctcagt
     6181 gatggccttc gcggccgcgc tgttgctgtt cggctcccga ggcccggcgc gccgcggggc
     6241 cgtcggctcg gctttcgtcc ccgcacccac ctcggcggcg gccgcggagt tcagcccgtg
     6301 gaagctggcg tcgaggatgg tgacggagga gcgcggcgag ccgacgggca gacaggcgaa
     6361 ggtcgaagtc cccggacagc tccgccacta cagcgacacg cgccgcttcc tcgccattca
     6421 ggtggccgag tggaaggagc acggcgtcgc cacgccccac gactacgccg acctcgccgc
     6481 gctcatccgc gccggcgagc tggtcgaagt cccgaccgtg ggcgacagct acgtcctctt
     6541 cggcgtcggc ggcagcgccg acaagcagct ctt
//
LOCUS       DASVES010000048         4648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1507175, whole genome shotgun sequence.
ACCESSION   DASVES010000048 DASVES010000000
VERSION     DASVES010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507175"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1411)
                     /locus_tag="VF240_01930"
     CDS             complement(308..1411)
                     /locus_tag="VF240_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7174030.1"
                     /translation="MTTSGARHDRVRSSEYQLWAKTRARTRFDLAVSGIVNFPLADLP
                     VRPEDLELSGPSLYGYAPLQEALARKCGVPAECVVHATGTSMANHLVMAALLDPGDEV
                     LIESPAYEPMVATARYLRADVKRFARRAEDNFAVRPSEVERNLSLRTRLVVLTNLHNP
                     SSAHTDEDTLRQIGLMARAVGARVLVDEVYLDALFEHAPPTAFHLGEDFIVTSSLTKV
                     YGLSGLRCGWILAEQALAQKLWRLNDLFGVIPAHPAERLSVAALRNLDTIAARSRTLL
                     ETNGQLLTSFLRTRDDLEYFEHRSGTVSFPRFKVGDVDDLGRLLVEKFETSVVPGRFF
                     GMPSHMRIGISCDTEMLRGGLERLGEALDDVKR"
     gene            complement(1408..2982)
                     /locus_tag="VF240_01935"
     CDS             complement(1408..2982)
                     /locus_tag="VF240_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7174031.1"
                     /translation="MWKPPRVREYYRTRRRAPEVPKRPAAPPEQQPARNTGLINTNGG
                     GAQHAGTGPRIEVGTGPPLARRLGLFDATMIVMGGIIGAGVFVNPHVVARQVHTPALI
                     LGVWLAGGLIALVGAFIYAELAARRPEVGGQYAYLRDAYHPAVAFLYGWALLLVVQTG
                     GMAAVAITFSRYFLEVTRAPIPDWAVAAAALGMLTVVNCFGVRAGGSLQSVLMVLKIA
                     ALAALIIIGLLLARGPALTTAPLLDRPASLGLVAAMGAALTPVMFAYGGWQTASFVAG
                     EMRDPRRDLARGLLLGVCGVIVLYTAVSFVCVYALGAEGLAATPTPASAVMRRALGER
                     GATLIAIGIAISTLGFLSQGMLTAPRVYFAMARDGLFFRRVAWVDPRSRAPVVAIALQ
                     GLLAVVIAVSGSYEQILNYVVSVDFIWFGLTAASIFVFRRRTTTTGDAVEVSETVDAR
                     AVSDGKEVAADSVEDRSGGFRVPGHPWTTAFFVAACALIVISTVYEHLADSGVGLLIV
                     AAGVPVYYFWSRRRKG"
     gene            3024..4310
                     /locus_tag="VF240_01940"
     CDS             3024..4310
                     /locus_tag="VF240_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX7174032.1"
                     /translation="MTQTDTALNQINHETAAEATHAVLPTEEIRSHFPALERTHNGHA
                     VAYFDGPGGTQAPRSVVAAMSDYLFGHNANTHWAYPTSEETDRLIESARSTLADFLGA
                     APEEIVFGANMTTLAFHLSRALAPGIQEGDEIVVTELDHHANLAPWQRLASERDASLR
                     VVRMRPETGELDWDDFERKVGARTRLVAVGAASNALGTINDLARATRLARAAGALVFV
                     DAVHFAPHAPVDVRALDCDFLACSAYKFYGPHIGVLYSRRRLLETIDFPKLQPAPDAA
                     PERAETGTQNHEGMLGAAAAVDFLASLAAGESRRERLRVSFEELHRRGAALLREMWTG
                     LSEVEGVRMFGPPVTSARTPTVAFTVAGVASTEVARRLAERGVFVSHGDFYAATVVER
                     LGLGDEGLVRAGCACYTTTEEVARLVEGVREIARGA"
     gene            4439..>4648
                     /locus_tag="VF240_01945"
     CDS             4439..>4648
                     /locus_tag="VF240_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174033.1"
                     /translation="MLLTWCCLLGMLVLGCSSVADDGEAKDVGPRQVELRVGEETTLP
                     DSKIKIRFLSVDNDSRCPQGVNCIWA"
BASE COUNT          817 a         1445 c         1645 g          741 t
ORIGIN      
        1 gagggccgac gaggctaccg acatgccgat gcacatccaa gcccaatagt ccgggtatcg
       61 cctctcgggc ggggcaaact aatagaacca tacgaccgaa aacatctggt attgataaga
      121 ggcagaccgc cgcgctacat aggaagccca gagcgtacag caatcttctc gtaaacttgc
      181 tactgggtct catacctcac ctgggcgaaa gaaatctaac gctcgttagg ccgccagtgg
      241 cggtatttta cctgaataag gcgacaatta ccgccgttgg tggcctacaa ggagcggctt
      301 gataacttca ccgcttaaca tcgtccagcg cctcgcccaa tcgttctaat cctcctctca
      361 acatctccgt gtcgcaactg atgccgattc gcatgtgcga gggcattccg aaaaagcgcc
      421 ccggcacgac tgaggtttcg aacttctcga ccagaagccg gcccaggtcg tcaacgtcgc
      481 cgactttgaa gcgggggaag gagacggtgc cggagcggtg ctcgaagtat tcgaggtcgt
      541 cgcgggtgcg gaggaatgag gtgagcagtt ggccgttggt ttccagtagg gtgcgcgagc
      601 gggcggcgat tgtgtcgagg ttgcggaggg cggcgacgct gaggcgttcg gcggggtggg
      661 cggggatgac gccgaagagg tcgttgagac gccagagttt ttgggccagt gcctgctcgg
      721 cgagaatcca gccgcagcgg aggccgctga ggccgtagac ttttgtgagg ctcgacgtga
      781 cgatgaagtc ttcgccgaga tgaaacgcag ttggcggcgc gtgctcgaac agggcgtcca
      841 gatacacttc gtcaacgagg acgcgcgccc ccacggcgcg ggccatcaag ccgatttgtc
      901 ggagtgtgtc ttcgtcggtg tgtgcgctcg aagggttgtg gaggttcgtg aggacgacga
      961 gacgggtgcg gagagacagg ttgcgttcga cctcggaggg gcggacggcg aagttgtctt
     1021 ccgcgcggcg ggcgaaacgc ttcacgtcgg cgcggaggta gcgcgccgtg gcgaccatcg
     1081 gctcgtaggc cggtgattcg atgaggactt cgtcgccggg gtcgagcagt gcggccatga
     1141 cgaggtggtt ggccatcgac gtgcccgtcg cgtggacgac gcactcggcg gggacgccgc
     1201 actttcgggc gagcgcttcc tgcaagggcg cgtagccgta gaggctcggc cccgacaact
     1261 ccaaatcttc aggccgcacg ggtagatcgg cgagggggaa gttgacgatg ccgctcacgg
     1321 cgaggtcgaa gcgcgtgcgc gctcgcgtct tcgcccaaag ttggtactcg gaactcctga
     1381 cgcggtcgtg ccgcgcgccg ctggttgtca tcctttcctc cgcctgctcc aaaagtaata
     1441 aacggggacg ccggccgcga cgatgagcag gccgacgccg ctgtccgcga ggtgttcgta
     1501 gacggtgctg atgacgatga gcgcgcacgc cgcgacgaag aaggcggtcg tccacgggtg
     1561 gccggggacg cggaagccgc ccgacctgtc ttcgactgaa tcagccgcga cttctttgcc
     1621 gtcgctcacc gcgcgcgcgt caacagtttc agacacttcg accgcgtcac ccgtcgtcgt
     1681 cgtccgtcgg cggaagacga agatggaggc ggcggtcaga ccgaaccaga tgaagtctac
     1741 ggagaccacg tagttgagta tctgctcata gctgccggag acggcgatga cgacggcgag
     1801 cagaccttgc agcgcgatgg cgacgacggg tgcgcgcgag cgcggatcga cccacgcgac
     1861 gcggcggaag aacagtccgt cgcgggccat tgcgaagtag acgcgcggcg ccgtgagcat
     1921 cccctgactg aggaagccga gggtggagat ggcgatgccg atggcgatga gcgtcgcgcc
     1981 gcgctcgccg agggcgcgac gcatcacggc cgaggccggc gtcggcgtcg cggccaaccc
     2041 ttcggcgccg agcgcgtaga cgcagacgaa gctgacggcc gtgtagagca cgatgacgcc
     2101 gcagacgccg aggagcagcc cgcgcgcgag gtcgcgccgg gggtcgcgca tctcgccggc
     2161 gacgaagctt gccgtctgcc agcccccgta ggcgaacatc acgggcgtca gcgccgcccc
     2221 catcgcggcg acgagtccga gcgaggccgg tcggtcgagc aagggggcgg tcgtgagcgc
     2281 cggcccacgc gcgagcagta gcccgatgat gatgagcgcg gcgagggccg cgattttcaa
     2341 aaccatcagc acgctctgga gactcccgcc ggcgcgcacg ccgaaacagt tgaccacggt
     2401 gagcatgccg agcgcggcgg cggcgaccgc ccagtcgggt atgggcgcgc gcgtcacctc
     2461 cagaaagtag cgcgagaagg tgatggcgac ggcggccatc ccgcccgtct gcaccacgag
     2521 cagcagcgcc cagccataga ggaaggccac ggccgggtga tatgcgtcgc gcagatatgc
     2581 gtactgcccg cccacctcgg ggcgacgcgc cgccagctcc gcgtagatga aggccccgac
     2641 gagcgcgatg agaccgcccg cgagccacac gccgaggatg agcgcgggcg tgtgtacctg
     2701 tcgggcgacg acgtgcgggt tgacgaagac gcccgcgccg atgatgccgc ccatcacgat
     2761 catcgtcgcg tcgaagagtc cgagtcgccg cgcgaggggc ggccccgtgc cgacctctat
     2821 gcgtggccca gtcccggcgt gttgcgcgcc gcctccgttt gtattaatca atccggtgtt
     2881 cctcgcgggc tgctgttcgg gtggagcggc cgggcgcttc ggaacttccg gggcgcgccg
     2941 gcgcgtgcga tagtactccc gaacgcgtgg gggtttccac attaacaagc acgtccgttt
     3001 caaaatcatc ggggagtgaa tcattgactc agacagacac ggcactaaat caaatcaacc
     3061 acgagacggc ggccgaagcg acgcatgcgg tgctgcccac agaggaaatc aggtcgcact
     3121 tccctgccct cgaacgcacg cacaacggtc acgcggtcgc ctacttcgac ggccccggcg
     3181 gcacgcaggc gccgcgctcg gtcgtcgccg cgatgagcga ctacctcttc ggccataacg
     3241 ccaacaccca ttgggcctac ccgacgagcg aggagaccga ccggctcatc gagtcggcgc
     3301 gctcgacgct cgccgacttc ctcggcgccg cgcccgaaga gatcgtcttc ggcgccaaca
     3361 tgaccacgct cgccttccat ctgtcgcgcg ccctcgcgcc ggggatacaa gagggcgacg
     3421 agatcgtcgt cacggaactc gaccaccacg ccaacctcgc gccgtggcag aggctcgcga
     3481 gcgaacgcga cgcaagcctg cgcgtcgtgc ggatgaggcc ggagaccggg gagcttgact
     3541 gggacgactt cgagcgcaag gtcggcgcgc ggaccagact cgtcgccgtc ggcgccgcct
     3601 cgaacgcgct cggcacgatc aacgacctcg cgcgcgcgac ccgtttggcc cgcgccgccg
     3661 gggcgctcgt cttcgtcgat gcggttcact tcgccccgca cgccccggtg gacgtgcgcg
     3721 ccctcgactg cgatttcctc gcctgctcgg cctacaagtt ttacgggccg cacatcggcg
     3781 tcctctactc ccgccgcagg cttctggaga cgattgactt ccccaagctt caaccggcgc
     3841 cggacgccgc gcccgagcgc gcggagacgg ggacgcagaa tcacgaggga atgttggggg
     3901 ccgccgccgc ggtcgatttc ctcgcctcgc tcgccgcagg agagtcgcgc cgggagcgtc
     3961 tgcgcgtctc gttcgaggaa ttgcaccgaa ggggcgcggc gcttttgcgc gagatgtgga
     4021 cggggctgtc cgaggtcgaa ggcgtgcgga tgttcgggcc gcctgtgacg tcggcgcgaa
     4081 ctccgaccgt cgccttcacg gtcgcgggcg tcgcatcaac ggaagtggcc cgccgactcg
     4141 cggagcgcgg cgtcttcgtc tcccacggcg acttctacgc ggcgacagtg gtcgagcggc
     4201 tgggtctcgg cgatgaggga ttggtgcgcg ccgggtgcgc ctgttacacg acgaccgagg
     4261 aggtcgcgcg gcttgtcgaa ggtgtgcggg agattgcgcg gggcgcgtga gtgaagcgcg
     4321 tgcgcgccgg cggcggtaat ttcgccgggc acgacggagc gtgcggcgcg ggcgtgtcat
     4381 cttctcggaa gtgttgtatt tcttttcaac ggaagattac gagaggggga ctaaggggat
     4441 gcttttgaca tggtgctgct tgctggggat gttggtgctg gggtgtagta gcgtagccga
     4501 cgacggggag gcgaaggatg tggggccgcg gcaggtcgag ctgcgagtgg gggaggagac
     4561 gacgctgccg gactcgaaaa tcaagatcag gtttctctcc gtggataacg actcgcggtg
     4621 cccgcagggc gtcaactgca tctgggcc
//
LOCUS       DASVES010000049         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_536788, whole genome shotgun sequence.
ACCESSION   DASVES010000049 DASVES010000000
VERSION     DASVES010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536788"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..2437
                     /locus_tag="VF240_01950"
     CDS             404..2437
                     /locus_tag="VF240_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174034.1"
                     /translation="MENISIGERVEAESASPSLPAERVRAETGVRDRTILFVVGALLI
                     GFSFWWTQFSTQAICCGDYDGYYHFRLARMLQDGMRSGQFPPAFEALPLTTLNASDYV
                     DHHFLFHVLQIPFTWFDDARAASWAVLLLLLLASPLVYRHALRLIHAGNHHFVAGAVS
                     TVAGLVVVMALIQTRGGTLWKLGASVIIVATIGLCGWLTTQLLARGRARPVAALAALS
                     VALLGPLVFFRATTFYQSRAADFFAGDPYANFHYGAKLGTWLFACLAVMACFWMVMRY
                     RLRYPVFWLLALLGCSTPFLYRMNMSKAMSVSIVLLIVGIHLLFRRKYLWLLPLAYVF
                     ALTYDMVLLLWVAAFAWLAVNVWVEDWELTSKPVVRAAGGLLLVVAGTALGFVINPYF
                     PRNVQLMYEHMAMKITAGEFSTAVGGEWYPYNTWEFFWNCFVACLSMIVGYIAFKTTE
                     DRKQIAHALFFLLFATFLMFVNARWKRFSEYWPPFAVLFVAFTLQPYLQAWRGALETE
                     EGRAGRGERWLKYGMTAFTLAVIAVPAVWAAHITAQDIKGNDPNNQYLGGMEWVRANV
                     PPGEMIFNTDWDDFPKLFFYSPEHRYSSGLDPTYLLDRDKELGELYARIGLGEETNPG
                     PPIRERFGARYVFTDKHHGAFMQNALDSGWFVKEYEDDNSIVLRVLDESVKQE"
BASE COUNT          384 a          842 c          731 g          498 t
ORIGIN      
        1 gagcaggtcg tcgcgcgtct caaagagatc ggcgaagtgc agaaggccga aagccccggt
       61 aggggcgtaa gcatgtagcc cacggccgcg agccgtgggg acagacgcga cacaaaatcc
      121 aaagccccga aggggcgaaa gaatctcccg tcgagatttc tttcgcccct tcggggcttc
      181 aaaacttttc gggcgtcatt tccacggccc gacgccgtgg gctacactct ttcgccgctg
      241 acgcggcttt gagtcgcctg tgctgtgaac ttatcgtccg tcgcgcggcc ggttgtttgg
      301 atttcgctgt ttcttacgcg acaggccgcc gtgttatttt gtccgtcgtt cccggtgaat
      361 ggtcgcgccg acgggcgcgt ttgcgaggtg gctcaatcta gtcatggaaa atatcagtat
      421 cggagagcgc gtcgaggcgg agagcgcgtc gccgagtctc cccgccgagc gggtgcgcgc
      481 cgagacgggc gtgcgcgacc gcaccatcct ctttgtcgtc ggcgcgctcc tcatcggctt
      541 ctccttctgg tggacgcagt tttccacgca ggcgatctgc tgcggcgact acgacggcta
      601 ctaccacttc cgtctggcgc ggatgcttca ggacgggatg cgctccggcc agttcccccc
      661 ggccttcgag gcgttgccgc tgaccacgct caacgcctcg gactacgtcg atcaccactt
      721 cctctttcac gtcctccaga ttcccttcac ctggttcgac gacgcgcgcg cggcctcctg
      781 ggccgtgctg ctcctcctcc tgctcgccag cccgctcgtc taccgccacg cgctgcgtct
      841 gattcacgcc ggcaatcatc acttcgtcgc cggggcggtc tcgaccgtgg ccggtctcgt
      901 cgtcgtcatg gcgctcatcc agacgcgcgg aggaacgctc tggaaactcg gcgcctccgt
      961 catcatcgtc gcgaccatcg gactgtgcgg ctggctgacg acacagttgc tcgcacgcgg
     1021 gcgagcgcgc cccgtggccg cgctcgccgc attgtcggtc gcgctcctcg gccctctcgt
     1081 cttcttccgc gccacgacct tttaccagtc gagggcggcc gacttcttcg ccggcgaccc
     1141 ctacgccaac ttccactacg gcgcgaagct cggcacctgg ctcttcgcct gtctcgccgt
     1201 catggcctgc ttctggatgg tcatgcgtta tcgactgcgc taccccgtct tctggctcct
     1261 cgcgctcctc gggtgctcga cgccgttcct ctaccggatg aacatgagta aggcgatgag
     1321 cgtctccatc gtgctgctca tcgtcggcat ccacctgctc ttccggcgca agtatctgtg
     1381 gctgctgccg ctggcctacg tcttcgcgct cacctacgac atggtgctgc tgctgtgggt
     1441 cgccgccttc gcgtggctcg ccgtcaacgt ctgggtggag gactgggagc tgacctcgaa
     1501 gccggtcgtg cgggccgctg gggggctgct gctcgtcgtg gccggaaccg cgctggggtt
     1561 cgtgattaac ccatacttcc cgcgcaacgt gcagctcatg tacgagcaca tggcgatgaa
     1621 gatcacggcc ggggaattct cgacggccgt cggcggggag tggtatccgt acaacacctg
     1681 ggagtttttc tggaactgct tcgtcgcctg tctctcgatg atcgtcggct acatcgcctt
     1741 caagacgact gaagaccgca agcagatcgc ccacgcgctc ttcttccttc tcttcgccac
     1801 gttcctgatg ttcgtcaacg cgcgctggaa acgcttctcc gagtactggc ccccattcgc
     1861 cgtcctcttc gtggccttca cgctccagcc atatctacag gcgtggcgcg gcgcgcttga
     1921 gaccgaggag ggacgggccg ggcgcggtga gcgttggttg aagtacggga tgactgcgtt
     1981 cacgcttgcc gtcatcgccg tgcccgcggt ctgggccgcg cacatcacgg cgcaggacat
     2041 caagggcaac gacccgaata accagtacct cggcgggatg gagtgggtgc gcgcaaacgt
     2101 accccccggc gagatgatct tcaacaccga ctgggacgac ttccccaaac tcttcttcta
     2161 ctcgcccgag caccgctact cgtcgggcct cgacccgacc tacctgctcg accgcgacaa
     2221 ggagctgggc gaactctacg cccgcatcgg cctcggcgaa gagaccaacc ccggcccgcc
     2281 catccgcgaa cgcttcggcg cgcgctacgt cttcaccgac aaacaccacg gcgccttcat
     2341 gcagaacgcg ctcgacagcg gctggttcgt caaagagtac gaggacgaca actccatcgt
     2401 cctgcgcgtc ctcgacgaat cagtcaagca ggagtagaac acgggtaaga cggat
//
LOCUS       DASVES010000050         3376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_536370, whole genome shotgun sequence.
ACCESSION   DASVES010000050 DASVES010000000
VERSION     DASVES010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536370"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VF240_01955"
     CDS             <1..1040
                     /locus_tag="VF240_01955"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX7174035.1"
                     /translation="LEAWGLGNARELSGVAPVENPRGSYGRCATRSNGKDTTTGHWEM
                     AGIILDEAFPTYPQGFPAEVLDRFVEAAKVPGVLGNFPASGTEIIKELGAEHVRTGKP
                     IVYTSADSVFQIAAHEEIIPVERLYEICEAARRVLRGEHDVGRVIARPFLGEPGAFYR
                     TENRHDYAVPPPRENLLPTLSDAGFDVVSVGKIASIYDSVGVTFDLKAKNNDQSIDQT
                     IRALRDDSRGLIFSNLVDFDMLYGHRRDVEGYARALEHFDARLPEIEAAMRDTDLLLI
                     TADHGNDPTYRGTDHTREYVPLLAYGRTARAGINLGDRASLADIGQTIADNFGLRLSA
                     GESFLSAITGA"
     gene            complement(1323..2342)
                     /locus_tag="VF240_01960"
     CDS             complement(1323..2342)
                     /locus_tag="VF240_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7174036.1"
                     /translation="MQRLAALLITASLSAGTALAHEHGHRAPQGDMSKVEIKTEKVAG
                     SVHMLQGAGGNIGVTSGPDGLLIVDDQYAPVSEKIRAALKQLNPGRLKFVLNTHWHGD
                     HTGGNAAFGKESTIIAHENVRKRMATTSKVFGESVPPSPPEALPVVTFDESVHLHFNG
                     EEINVIHFPAGHTDGDSIIFFTGSNVIHMGDHFFAGRFPFVDLENGGNVEGLTRNVAE
                     VIAKAPAGVKIIPGHGPVSTVEDLKTYHGMLVETTGIVRERMKAGKTLAQIKAEGLPE
                     KWKTWGAGFIKTDLWLETIYQSLTRDGEKKAAALLGVRDFHESLFARPAPQRTFKVRR
                     IDTEL"
     gene            complement(2383..2952)
                     /gene="hpt"
                     /locus_tag="VF240_01965"
     CDS             complement(2383..2952)
                     /gene="hpt"
                     /locus_tag="VF240_01965"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX7174037.1"
                     /translation="MSSSSVRAASEFTNSNLEPLFSAEQIQARIAEMGADIAREYAGR
                     NPLLVGVLKGACVFLSDLVRAIDTRIGLEFMAISSYGSSTRSSGEVRIVKDLDVPVEG
                     RDILVVEDIVDTGLTLSYLLQTLHTRGARSVKLAALLDKWERREREVEIDYVGFRIPD
                     AFVVGYGLDFAERYRNLPYIAVLKDPNAD"
     gene            3183..>3376
                     /locus_tag="VF240_01970"
     CDS             3183..>3376
                     /locus_tag="VF240_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174038.1"
                     /translation="MADDTQSTRERVRALVRDVLENALPAEDGGGAAGSPSEQATPGV
                     RGGVDLAATPRARVIQTAPA"
BASE COUNT          585 a         1092 c         1120 g          579 t
ORIGIN      
        1 accttgaagc gtggggcttg ggtaacgcgc gagagctgag cggagtcgcg ccggtcgaga
       61 atccgcgcgg gtcatacggc cggtgcgcga cgcgctcgaa cgggaaggac acgacgacgg
      121 gccactggga gatggccggc atcatcctcg acgaagcctt ccccacctac ccgcaggggt
      181 tccccgcgga ggtgctggac aggttcgtcg aggcggcgaa agtgccgggc gtgctcggca
      241 acttccccgc gtcggggacg gagatcatca aggagctggg cgcggagcac gtccgcacgg
      301 gcaagcccat cgtctacacc tcggccgact cggtcttcca gatcgcggcg cacgaagaga
      361 tcatcccggt cgaacggctc tacgagattt gcgaggcggc gcggcgcgtg ctgcggggcg
      421 agcacgacgt gggccgcgtc atcgcgcgcc cattcctagg cgaacccggc gcgttctacc
      481 gcaccgagaa ccgccacgac tacgctgtgc ccccgccgcg cgagaaccta ctgcccacgc
      541 tctccgacgc cggattcgac gtggtctccg tcggcaagat cgcctccatc tacgactcag
      601 tcggcgtcac cttcgacctc aaggccaaga acaacgacca gtcaatcgac cagaccatcc
      661 gcgcgcttcg cgacgactcg cgcggcctca tcttctccaa cctcgtcgat ttcgacatgc
      721 tctacggcca ccgccgcgac gtggaaggct acgcccgcgc cctcgaacac ttcgacgcgc
      781 gcctgcccga gatcgaggcg gcgatgcgcg acacagacct cctcctcatc accgccgacc
      841 acggcaacga ccccacctat cgcggcaccg accacacgcg cgagtacgtc ccccttctcg
      901 cctacggccg cacggcgcgc gccggcatca acctcggaga ccgcgcctcg ctcgccgaca
      961 tcggccagac catcgccgac aacttcggcc tgcgcctcag cgcgggcgag agttttctga
     1021 gcgcgataac gggcgcatga cgacacgcgc ggcgacgcgt gagcgagcac cggcgtcaag
     1081 gagccgcgga gcggcggaag aatgtagcca cgggcgttag cccgtggaat agacgtaaat
     1141 aaaaattgga agccccggag gggcgaaaga atcttgacgg gcaccacgcc caatctaaag
     1201 attctttcgc ccctccgggg cttcatatta tttcgccgcc tgcccccacg gctggcgccg
     1261 tgggctacat tcttccgccg ctttcagcgg ctcgttgatg ttcgtgtcta cacttggcgc
     1321 ggctacaatt ccgtgtcaat gcgtcgcacc ttgaatgtgc gttgtggcgc ggggcgcgcg
     1381 aagagggact cgtgaaaatc gcgcacgccg agcagggcgg cggccttttt ctcgccgtcg
     1441 cgcgtgaggc tctggtagat ggtttcgagc cacaggtcgg ttttgatgaa gcccgcgccc
     1501 cacgtcttcc acttctcggg cagcccctcg gccttgatct gcgccaaagt cttccctgcc
     1561 ttcatgcgct cacgcacgat tccggtcgtc tcgacgagca tcccgtggta ggtctttaaa
     1621 tcctccacgg tcgagacggg gccgtggccg gggatgatct tcacgcccgc cggggccttc
     1681 gcgatcacct cggcgacgtt gcgcgtcagc ccctcgacgt ttccgccgtt ctccagatcg
     1741 acgaagggga agcgcccggc gaagaagtgg tcgcccatgt ggatgacgtt cgagccggtg
     1801 aagaagatga tgctgtcgcc gtcggtgtgg ccggcgggga agtggatgac gttgatttcc
     1861 tcgccgttga agtggaggtg gacggactcg tcgaaggtga cgacgggcag cgcctcgggc
     1921 ggcgagggcg ggacgctctc gccgaacacc ttcgacgtgg tcgccattcg cttgcggacg
     1981 ttctcgtggg cgatgatggt cgattccttg ccgaaggcgg cgttgccgcc ggtgtggtcg
     2041 ccatgccagt gggtattgag gacgaacttc agacgccccg ggttgagctg tttgagcgcc
     2101 gcgcgaatct tctcggagac cggcgcgtac tggtcgtcca cgatgagcag cccgtcgggg
     2161 ccggacgtga cgccgatgtt cccgcccgcg ccctgaagca tgtggacgct gccggccacc
     2221 ttctcggttt tgatttcaac cttcgacatg tcgccctgcg gcgcgcggtg tccgtgctcg
     2281 tgcgcgagcg ccgtgccggc cgagagagac gcggtgatta agagtgcggc aagtctctgc
     2341 atctgattcc tccgtgatgt cgggcgccgc gcgcgggcgc tttcagtcgg cgttcgggtc
     2401 tttgaggacg gcgatgtagg gcaggttgcg gtagcgctcg gcgaagtcga ggccgtagcc
     2461 gacgacgaag gcgtcgggga tgcggaagcc cacgtagtcg atctccactt cgcgctcgcg
     2521 ccgctcccac ttgtcgagca gggcggccag cttgacgctt cgggcgccgc gcgtgtggag
     2581 cgtctgaagc aggtaggaga gcgtcagccc ggtgtccacg atgtcttcga cgacgaggat
     2641 gtcgcgcccc tcgacgggca cgtccaaatc cttcacgatc cgcacctcgc ccgacgagcg
     2701 cgtgctgctg ccgtagctcg aaatcgccat gaactccagc ccgatgcgcg tgtcgatggc
     2761 gcgcaccaga tcggagagaa agacgcacgc gcctttgagg acgccgacga gcagcgggtt
     2821 gcggcccgcg tactcgcgcg cgatatcagc gcccatctcc gcgatgcgcg cctgtatctg
     2881 ctcggcgctg aagagcggct caaggttaga gttggtgaat tcagaagcgg ctcggacgga
     2941 cgaagaagac aaggcgacgc ctcccacgcg ggttgaaagt tcgagaaccg aaacgacggc
     3001 gaaccggcaa gtttgcaccc gcgactcttg gtgcgacata ctaaggagcc ttacgtggaa
     3061 ggtcaaacga aagtgagcag tgagcagtaa gcagtaagca gtaagcagtt ggacgcttac
     3121 gtagcttgcc gctcatcgcc gaacttgttt ttactgctca ctgcttactg ctcactgctc
     3181 ttatggccga cgacacacaa tcgacgcgcg agcgcgtgcg ggcgctcgtg agagacgtgc
     3241 tggagaacgc gctcccggcg gaggatgggg gaggcgcggc ggggtcgccg tccgagcagg
     3301 cgacgccggg cgtccgcggg ggtgtggact tggcagcgac gccgcgggcg cgcgtcatcc
     3361 agacagcgcc ggcgga
//
LOCUS       DASVES010000051         7819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_143625, whole genome shotgun sequence.
ACCESSION   DASVES010000051 DASVES010000000
VERSION     DASVES010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143625"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..2258
                     /locus_tag="VF240_01975"
     CDS             72..2258
                     /locus_tag="VF240_01975"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX7174039.1"
                     /translation="MIRIDEIVEKVGRNHPQADLDLLRRAYIFSARWHRGQKRASGEP
                     YLVHPLEVANILAEMRLDETSVATGLLHDVVEDTLVESDVIREQFGDEIAHLVDGLTK
                     IAQISSLSREEQQAENVRKMLLAMVDDVRVVLIKLADRLHNMRTLHHLRPDKRKRIAQ
                     ETLDIYAPIAHRLGMGRVRGELEDLAFKHLHPEDFQELAAQLERRRPEHEAFLKEITA
                     RIEEKMGEADVPVLRVEGRVKRLYSIWKKLRRQKIALDQVYDLIAARVVTPDDVRHCY
                     AALGVIQTTWQPIPGRIKDWIATPRDNLYRSLHTSVIGPKGQAFEIQIRTDEMHRTAE
                     EGVAAHWKYKEGRRGAREDDEAFQWLRSLVEWSQEVKDSREFLESLKVDLYPKDVYAF
                     TPMGKVIQLPRGATPVDFAYMIHSEVGDRCTGARINSRMVPMRTELQNGDVVEILTNP
                     NAHPSRDWLNFVKTSRARNRVRHWVAEQQRADSIEIGRKLFEKEAGKFNLSAKKLLND
                     SNGEFKRVANEYGYGRTEDILAAIGYGKVMPRNVIAKFLGPEKFAELDQRKESKLRSG
                     VEAVKRLIRLGEDSIVVRGVDDLLVIRARCCSPVRGEEIIGYVTLGKGVAVHASRCKN
                     VQSLMVNPERVVEVEWAGKDDQSAYAVKLLAITENRTGMIAGITNVISDMKTGIREAR
                     ASVSSDNQGRIEVTVEVFDVKHLDKVMTSIRKVPGVLDVERIQGAA"
     gene            2522..2917
                     /locus_tag="VF240_01980"
     CDS             2522..2917
                     /locus_tag="VF240_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7174040.1"
                     /translation="MKQIVATDEAPRAIGPYSQAVIAGGMVFASGQIPLDPRTGEFVA
                     GGIREQTEQVMRNLSAVLEAAGSGLERVLKATVYLADMADFAEMNEVYGGFFREEPPA
                     RSTVQAARLPRDARVEIDVIALAGIRAEG"
     gene            complement(2989..3183)
                     /gene="rpmB"
                     /locus_tag="VF240_01985"
     CDS             complement(2989..3183)
                     /gene="rpmB"
                     /locus_tag="VF240_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7174041.1"
                     /translation="MAKRCEVCNKGPQFGNNVSHANNRTRRRFDPNLQSIRVQRPQGG
                     TVRMKVCTVCIKGGKVAKAA"
     gene            3382..4572
                     /locus_tag="VF240_01990"
     CDS             3382..4572
                     /locus_tag="VF240_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX7174042.1"
                     /translation="MQRVQKQFAIFFAVALCAGALAACQQGGGATGKGDAGLAATVNG
                     KPITLSEVDRIISQQTQGQQSKMSTIDQAAARLQVLDELIKQQLLFQRAEKEKLLPTD
                     QEIDSAVNQQKGRMTAEDWDKLLKQNNQTEQGIREEARKVLAIQKLQDKAFSKISIRD
                     QEITDFYNGNKEQFVNARGVQLADIVADAFDGGGAYRDDAKTETDAKAKIDRIHAQLK
                     AGADFATVARANSEDASSIRGGDVGFATEDDLRKTGFPQELITRFFDQNAMPVGGYTD
                     PIKFPDGRWIIFKLTNRQLANENLTLDSPGVRDRIREGISTQQKTILSEALVRDASSE
                     AQIVNHLATSMLNDPASLGGFRPAQPASTQSAASPAATASPAASAAATAAATATPAAR
                     NANN"
     gene            4728..6719
                     /locus_tag="VF240_01995"
     CDS             4728..6719
                     /locus_tag="VF240_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7174043.1"
                     /translation="MLFRLTDVFKSYGSQDVLRGTTVQINPGEHVGLVGRNGAGKTTI
                     FRLVTEEETPDSGEVARARGLSLGLLEQHVDFEPGLTVHEAALAAFRELQQIEHEMHA
                     LEHRMAEGGDDLDAVLERYSELQHEFERLGGFEYAARAESILQGLGFSRDTWAMETEK
                     LSGGQKNRLGLVRLLLAEPDVLLLDEPTNHLDVAAVEWLEEFLTSYKSAYVLISHDRY
                     FLDRACTRIVEVEAGRAASYHGNYSDFLVEREERREAQRRAYENQRHLIAKTEEFIRK
                     NLAGQKTKQAKSRRNMLARMERVEAVRGDTPQGNFQLKGVERTGSQTLIVKELSVGYP
                     GNVLAANMTFTLTRSESLGIIGPNGSGKTTFVKTVLGKLPPLAGETRWGTNVQVGYYA
                     QQLDDLDDRNEIIMELRRVASPAATAGELRGFLAKFLFTGDDVYKHVRDLSGGEKGRL
                     ALAKLIYSRVNVLVLDEPTNHLDIPSREALEEALDAYEGTIIAISHDRYFLDRIATQI
                     LALDGRGRAEYHDGDYTEYHDWKAAGGNATADASTAPSVVEKTGAVKGKPAKETPPAQ
                     PGPKPEGKKAQGGGKSDGKIRVVKKGKPERAREAQLIEADIAAAEKRLAALTEEMSSP
                     EVARSPQRFNALNAEYTRTDEGLKALFEEWERAAESANA"
     gene            7270..>7819
                     /locus_tag="VF240_02000"
     CDS             7270..>7819
                     /locus_tag="VF240_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX7174044.1"
                     /translation="MSEINVQIKGGASAQQVPAGTSVAEALKRLDRDAAKRALAARVN
                     GREVDLAYQLERPEGNGGGAASVEIEPVLPDTLDGLDVIRHSTAHLLAAAVLDLFPGT
                     KLGIGPALLDDPRYGFFYDLIAPRTLTDEDLPVIEKRMRDLAKRNLAYRREELPKHEA
                     LRVFGERDEPLKCELVEDKGGDH"
BASE COUNT         1533 a         2596 c         2479 g         1211 t
ORIGIN      
        1 agaggtcaga ggttagaggt cagtgaagac agccactaac ctctgacctc tgacctctga
       61 cctctgcttt tatgattcgc atcgacgaga ttgtcgaaaa ggtcgggcgc aaccacccgc
      121 aggccgacct tgacctgctg cggcgcgcat acatcttctc ggcgcgctgg caccgggggc
      181 agaagcgcgc ctcgggcgag ccgtacctcg tccatccgct cgaagtcgcc aacatcctcg
      241 ccgagatgcg tctcgacgag acctcggtcg ccacgggact gctccacgac gtagtcgaag
      301 acacgctcgt cgagtccgat gtcatccgcg agcagttcgg cgatgagatc gcgcacctcg
      361 tcgatgggct gacgaagatc gcgcagatca gcagcctgtc gcgcgaggag caacaggccg
      421 agaacgtccg caagatgctc ctcgcgatgg tggacgacgt acgcgtggtg ctcatcaagc
      481 tcgccgaccg cctccacaac atgcgtaccc tccaccacct gcgccccgac aagcgcaagc
      541 gcatcgcgca ggagaccctc gacatctacg cgcccatcgc ccaccgcctc ggcatgggcc
      601 gcgtccgcgg cgaactcgaa gacctcgcct tcaaacacct ccaccccgaa gactttcagg
      661 agctggccgc gcagctcgaa cgccgccgcc ccgagcacga ggcgttcctc aaagagatca
      721 ccgcgcgtat cgaggagaag atgggcgagg cggacgtgcc cgtcctgcgc gtcgaggggc
      781 gcgtcaagcg tctctactcg atctggaaga agctccggcg gcagaagatt gcgctcgatc
      841 aggtctacga cctcatcgcg gcgcgcgtcg tcacacccga tgatgtgcgc cactgctacg
      901 ccgcgctcgg cgtcatccaa accacttggc agcccatccc ggggcgcatc aaggactgga
      961 tcgccacccc ccgcgacaac ctctaccgct cgctccacac ctccgtcatc ggccccaagg
     1021 ggcaggcctt cgagattcag attcgcaccg atgagatgca ccgcacggcc gaggagggcg
     1081 tcgccgcgca ctggaagtac aaggagggcc gccgcggcgc gcgcgaagac gacgaggctt
     1141 tccagtggct gcgttcgctc gtcgagtggt cgcaggaggt caaggactcg cgcgagttcc
     1201 tggagtcgct gaaggtcgat ctctacccga aggacgttta cgccttcacg ccgatgggca
     1261 aggtgatcca gctcccgcgc ggggcgacgc ccgttgactt cgcctacatg atccacagcg
     1321 aggtgggcga ccgttgcacg ggcgcgcgca tcaacagccg gatggtgccc atgcggacgg
     1381 agctacagaa cggcgacgtg gtcgaaatcc tcaccaaccc caacgcgcac ccctcgcgcg
     1441 actggctcaa cttcgtcaaa acctcgcgcg cccgcaaccg cgtccgccat tgggtcgccg
     1501 agcagcagcg cgccgacagc attgagatcg ggcgcaaact cttcgagaag gaggccggga
     1561 agttcaacct ctcggccaag aagctgctca acgattcgaa cggcgagttc aagcgcgtcg
     1621 ccaacgagta cggctacggc cgcacggagg acatactggc ggccatcggc tatggcaagg
     1681 tgatgccgcg caacgtcatc gccaaattcc tcggccccga aaagttcgcc gagttagacc
     1741 agcgcaagga gtcgaaactc cgcagcggcg tcgaggccgt caagcgcctg atcaggctcg
     1801 gcgaagactc catcgtcgtg cgcggcgtgg acgatctact ggtgatacgc gctcgctgtt
     1861 gcagccccgt ccgcggcgag gagattatcg gctacgtcac gctggggaaa ggagtggccg
     1921 ttcacgccag ccgctgcaag aacgtgcagt cgctgatggt caaccccgag cgggtcgtcg
     1981 aggtcgagtg ggcgggcaaa gacgaccagt cggcctacgc cgtcaagctc ctcgccatca
     2041 cggagaaccg cacagggatg atcgccggca tcaccaacgt catctcggac atgaagaccg
     2101 gcatccgcga ggcgcgcgcc agcgtctcgt ccgacaatca gggccgcatc gaggtcacgg
     2161 tcgaggtctt cgacgtgaag cacctcgaca aggtgatgac ctcgatccgc aaagtccccg
     2221 gcgtgctcga tgtcgagcgc attcagggcg cagcgtgacc ggagcactca gccgtcagcg
     2281 ttcagcactc agccagacac cagcccggca actcaaatac aattcaaagc cgcccggcgt
     2341 cggagcatcc cggcgcgccc cgcgcacatc aattaattcg taccacgcag ttcgccccaa
     2401 tttttaaggc tgagagctga ccgctaattg ctgacggcta gtgcctgatt gctgacagcc
     2461 gacggcttct gttagaatac gcagtttgtt ttgagagtct gattcgaggt tatcaaagac
     2521 agtgaagcag atcgtcgcga cggatgaggc gccgcgggcc atcggcccgt attcacaggc
     2581 ggttattgca ggcgggatgg tcttcgcctc cggccagatt ccgctcgacc cgcgcacggg
     2641 cgagttcgtc gcgggcggca tccgcgagca gacagaacag gtaatgcgga acctttcggc
     2701 cgtgttagag gcagccgggt cggggctgga acgggtattg aaggccacgg tctacttggc
     2761 ggacatggcc gattttgcgg agatgaacga ggtctacggc gggttctttc gtgaggagcc
     2821 gcccgcgcgc tcgaccgtgc aggccgcacg cctgccgcgc gacgcccgcg tcgagatcga
     2881 cgtgatcgca ctggcaggga tcagggcgga aggatgaggg atgcataaga agaaggcgaa
     2941 ggccgagggc tttgattcat ccctcatccc tccgccctca tcccttgctt aagccgcctt
     3001 cgccaccttg ccgcccttga tgcagacggt gcagaccttc atacggacgg tgccgccctg
     3061 cgggcgctgg acgcgtatgg attgcagatt ggggtcgaag cgacggcggg tgcggttgtt
     3121 agcgtgcgag acgttgttgc cgaactgcgg ccccttgttg cagacttcac atcgttttgc
     3181 catgactgat ttttgatacc tccggtcgtt aaaaaaagcg cgccgttctc gcctctacac
     3241 cacaggtcgc ggaggccgtg tgcgctcgaa acaaaaagtt atagcaaacg cttttaggcg
     3301 gggtcaagtg aggcggccgg ccggcggcgc ccgctttcag gcatttttct caaccagagt
     3361 cgaaggagcg aaagagttaa agtgcaaaga gttcagaaac agttcgcaat cttcttcgcg
     3421 gtcgccctgt gcgccggcgc gctggccgcg tgccagcagg gcggcggcgc gacgggtaag
     3481 ggcgacgcgg gcctcgcggc caccgtcaac ggcaagccca tcacgctgag cgaggtggat
     3541 cgcatcatca gccagcagac gcaggggcag cagtcgaaga tgtccaccat cgatcaggcc
     3601 gcggcccgcc ttcaggtgct cgacgagttg atcaagcaac aactcctctt ccagcgcgcc
     3661 gagaaggaga agctgctgcc gaccgatcag gaaatcgact cggccgtcaa ccagcagaag
     3721 ggccggatga cggccgagga ctgggataag ctgctcaagc aaaacaacca gaccgagcag
     3781 ggcatccgcg aggaggcgcg caaggtgctc gccatccaga aactccagga caaggcgttc
     3841 agcaagatca gcatccgcga tcaggagatc accgacttct acaacggcaa caaggagcag
     3901 ttcgtcaacg cgcgcggcgt ccagctcgcc gacatcgtcg ccgacgcctt cgacggcggc
     3961 ggggcctacc gcgacgacgc caagaccgag acggacgcca aggccaagat cgaccgcatc
     4021 cacgcgcagc tcaaggccgg ggccgacttc gccacggtcg cccgcgccaa cagcgaggac
     4081 gcgagcagca tccgcggcgg cgacgttggc ttcgcgaccg aggacgattt gcggaagacg
     4141 ggcttcccgc aggagttgat caccagattc ttcgaccaga acgcgatgcc ggtcggcggt
     4201 tacaccgacc ccatcaagtt ccccgacggc cgctggatca tcttcaagct gacgaaccgc
     4261 cagctcgcca acgagaacct gacgctcgac agccccggcg tgcgcgaccg catcagggag
     4321 ggcatctcca cccagcagaa gacgattctc agcgaggcgc tcgtgcgcga cgcatcgagt
     4381 gaggcccaga tcgtcaacca cctggcgacg agtatgctca acgacccggc ctcgctcggc
     4441 ggcttccgcc cggcgcagcc ggcctccacg cagagcgcgg cgtcgcccgc cgctacagca
     4501 tcaccggcgg cgagtgcggc ggccacggca gccgccacgg ccacgcccgc cgcgcggaat
     4561 gcgaataatt agcggccact gagtcgcggc gcgggcgggg cgactcggcc cgcgccggat
     4621 ttaaggtgaa gcgggcttac gctgcccccg aacgcgcggc gccggcccgc ttcacgtgcg
     4681 tctgcgccga ggagtttgca agatagggat aggcgtttcg aggtctcatg ctgttccgat
     4741 tgaccgacgt gttcaagtct tacggctcgc aggacgtgct gcgcggcacc accgtgcaga
     4801 tcaaccccgg cgagcacgtc ggactggtgg gccgcaacgg cgcggggaag acgacgattt
     4861 tccgcctcgt gaccgaggag gagacgcccg acagcggcga ggtcgcgcgc gcgcgcggac
     4921 tcagcctcgg tctcctcgaa cagcacgtcg atttcgagcc ggggctgacc gtgcacgagg
     4981 ccgcgctcgc cgccttccgc gagttgcagc agatcgagca cgagatgcac gccctcgaac
     5041 atcgcatggc cgagggcggc gacgatctcg acgccgtgct cgaacgctac agcgaactcc
     5101 agcacgagtt cgagcgcctc ggcggcttcg agtacgccgc gcgcgccgag tcgatccttc
     5161 agggtctcgg cttctcgcgc gacacttggg cgatggagac cgaaaagctc tcgggcgggc
     5221 agaagaatcg gctggggctt gtgcgtctgc tgctcgccga gccggacgtg ttgctgctcg
     5281 acgagccgac gaaccacctc gatgtcgccg ccgtcgaatg gctcgaagag ttcctcacct
     5341 cctacaagtc cgcctacgtc ctcatcagcc acgaccgcta cttccttgac cgcgcctgca
     5401 cgcgcatcgt cgaagttgag gcgggccggg cggcgagcta ccacggcaac tactccgact
     5461 tcctcgtcga gcgcgaggag cggcgcgagg cgcaacggcg cgcctacgaa aaccagcgcc
     5521 acctcatcgc caagaccgag gagttcatcc gcaagaacct cgccgggcag aagacgaagc
     5581 aggccaagtc gcgccgcaac atgctggcgc gcatggagcg cgtcgaggcc gtgcgcggcg
     5641 acacgccgca ggggaacttc cagctcaagg gcgtcgagcg caccggcagc cagacgctca
     5701 tcgtcaagga gctgtcggtc ggctatccgg gcaacgtcct cgccgcgaac atgaccttca
     5761 cgctgacgcg tagcgaatcc ctcggcatca tcggcccgaa cggctcgggc aaaacgacct
     5821 tcgtcaagac cgtgctcggc aagctcccgc cgctcgcggg cgagacgcgc tggggcacca
     5881 acgttcaggt cggctactac gcgcagcagc tcgacgacct cgacgaccgc aacgagatca
     5941 tcatggagct gcgccgcgtc gcctcgcccg cggccaccgc cggcgagctg cgcggcttcc
     6001 tcgccaagtt ccttttcacc ggcgacgacg tgtacaagca cgtccgcgac ctctcgggcg
     6061 gcgagaaggg ccgcctcgcg ctcgccaagc tgatctactc gcgcgtcaac gtgctcgtcc
     6121 tcgacgagcc caccaaccac ctcgacatcc cctcgcgcga ggcgctcgaa gaggcgctcg
     6181 atgcctacga gggcaccatc atcgccatca gccacgaccg atacttcctc gaccgcatcg
     6241 ccacccagat actcgcgctc gacggccggg gccgcgccga gtatcacgac ggcgactaca
     6301 ctgagtatca cgactggaag gccgctggcg gcaatgcgac tgccgacgca tcgacggccc
     6361 cgtctgtggt tgaaaagacc ggggccgtga agggcaagcc ggcgaaggaa acgccgcccg
     6421 cgcagcccgg cccgaagccg gagggcaaga aggcgcaggg cggcggcaag tcggatggca
     6481 agatcagagt cgtcaagaaa gggaagccgg agcgcgcgcg cgaagcgcag ttaatcgagg
     6541 cggacatcgc ggcggcggag aagcgtctgg cggcgctcac cgaagaaatg tcttcccccg
     6601 aggtcgcgcg ctccccccag cgtttcaacg cgctcaacgc ggagtacacg cgcaccgacg
     6661 agggcttgaa ggcgctgttc gaggaatggg agcgcgccgc cgagtcggcg aacgcctgac
     6721 gcgccatgtt cagccattcg cgacggccgg cgactgttga gagcgttgct gattggacgt
     6781 ttccgcccgg cgcggcgcac gtcgcttgac acggcggaag tcgattgtta tcttacatta
     6841 tccgcttttg gtaagcgttt tgacagtaat gttcaggcaa gtagctcaag cggtagagca
     6901 ctacctgaca cggtaggggg ccggggttaa aacccgcgcc attcctgcca gttggttcaa
     6961 actttcgaca gcgagctccg gaagaccttt gccgggacta cagcagatga cgcgaactcg
     7021 aactacgttt cgattctaat ctggcggcga gccggccccc acgcggggcg ggcctcggcg
     7081 gcgagggcgc ttctgtcgaa tccgatctga tacatccgtc aactctattg acgggacact
     7141 aattcggttg gcgatttcaa actctccgcg gggcctgcgg cttttcgcgc atgactctcg
     7201 acgcgcgcgc gtctcagcgt ttcgggcgcg cagtcgtcgt gagttttaac ccgacggaga
     7261 ctatcagcca tgagtgagat aaacgtccag attaaaggcg gcgcgtcggc gcagcaggtg
     7321 ccggcgggca cgagcgtcgc cgaggccctg aagcgcctcg accgtgacgc ggccaaacgg
     7381 gcgctcgcgg cgcgcgtcaa cggccgcgag gttgacctcg cctatcaact cgaacgcccg
     7441 gaggggaacg ggggcggcgc cgcttccgtc gagatcgagc cggtgctgcc ggacacgctc
     7501 gacggcctcg atgtcattcg tcactcgacg gcgcaccttc tggcggcggc cgtcctcgat
     7561 ctcttccccg gcaccaaact cggcatcggc ccggcgctct tagacgaccc gcgctacggc
     7621 ttcttctacg acctgatcgc cccgcgcacg ctcaccgatg aagaccttcc cgtcatcgaa
     7681 aagcgcatgc gcgacctcgc caagcgcaac ctcgcctatc ggcgagagga gttgccgaag
     7741 cacgaggccc tgcgcgtctt cggcgagcgc gacgagcctt tgaagtgtga gctggtcgaa
     7801 gacaagggcg gcgaccatg
//
LOCUS       DASVES010000052        11568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1114595, whole genome shotgun sequence.
ACCESSION   DASVES010000052 DASVES010000000
VERSION     DASVES010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114595"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..3361)
                     /locus_tag="VF240_02005"
     CDS             complement(131..3361)
                     /locus_tag="VF240_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7174045.1"
                     /translation="MISREKNFKHQSRSRLAAAALLIMLLGTLARAQSATATLTGAVI
                     DEAGAVVPGVRITVLNLSTALERHAATNGEGVYVVPLLPVGRYNLTAQRDGFTTVEVR
                     AITLNVSDQLALRIRLRVGEIGESVTVVDNLEGLGVVREGPSVSAVVNRPFVENLPLN
                     GRSFQTLFELTPGTTLTHASFSEQGQFSAAGQRANANYFMVDGVSANIGVSAGAAPGQ
                     SASGSLPALTVLGGANNLVSVEALEEFRIQTSSYAPEFGRTSGAQISVVTRSGTNEWH
                     GSVFDYVRHEALDANDWFANSRDLPRARLRQHDFGATLGGPLRRDRTFFFASYEGLRL
                     RLPQVAITDVPSTAARQSAPAPLRPFFDAFPVPTGADLGNGFAEAAATYSDRARFDAA
                     SVRIDHVVMNRLTFFGRYNYAPSDTVQRGGVSLPDFTGQSLNTVGRTRLDTQTLTAGA
                     TFAPAPNVLGDLRFNWSRAAAETTLALDDFGGARAPADSFLFPPFATPDESGFQFILR
                     GGTNSNLGTGRSVNNLQRQLNSVGNLSVVRGRHHFRMGADFRRLAPVYGPLRYSQSVV
                     FGDTASNGVLAAISTGRAFEVRVGADAGPRTPVFTNFSAYAQDTARITPRLTITYGLR
                     WELNPPPRESNDNHPVVLTGLETADDGLVIGSTAGTPRLDLDLAPRGTPLWQTSYGNF
                     APRVGVAYRLLPERGTVLRGGVGIFYDLGTGQAAQAFGSVFPFAKEKVLSNVAFPLDP
                     AEAVPPPVNLNPPFGTIYAFVPSLRLPFAFQWNAGLEHPLGAAQSVSAAYVGASGRSL
                     LRQRALLRPEPRFTVVRVTDNSSSSRYDSLQLQFIRRLSGGLQAHASYTFARSTDNDS
                     DDSSSFLFTGSSDPEGEHGPSNFDVRHSLTAAVTYNLPRPFDGRATTAGRLFHGWAVD
                     TLLRARTAVPINVITRTGTVINELVEARRPDLVSGVPIYVDDPEAPGGRRLNRAAFVQ
                     VFGRQGTLGRNAVRGFGFSQIDLALRREFRLSERARLQLRAEVFNLFNRPSFGDPVAD
                     LANPRFGLSTQTLARSLGAGGANGGLSPLYQVGGPRSVQLALKVLF"
     gene            complement(3400..4038)
                     /locus_tag="VF240_02010"
     CDS             complement(3400..4038)
                     /locus_tag="VF240_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7174046.1"
                     /translation="MAALIMCPALSALAQSSDDHNRFEFFGGFSHNRVDTGGPGDGLA
                     FAGDEEHTGFNGFNASVVGNLSRYAGLKLDVSGHFKREADADFGICVDATTGGVCFSP
                     RAKASLYNFLGGVQLKDNAKGGRAKPFAHLLVGAAHAKVTFDNSACLEALGFACLTDE
                     SETGIAGAIGGGLDIRLSERLDFRAVQLDYNPTHLGSQTQHNFRVGIGIVIH"
     gene            complement(4234..7374)
                     /locus_tag="VF240_02015"
     CDS             complement(4234..7374)
                     /locus_tag="VF240_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7174047.1"
                     /translation="MVQRLPDIAPAAAASSAPAECHEDIFLARYAAMRGWALRLAEGD
                     RARAEDLVHDAYVHFTLARPPLREVENLDGYLFVMLRNLHTSQARRAARLRERMLPAI
                     DYDSASGAARAADPRDLARVHDELRACCRYACARMHTSKAGSVLALRFFHGYYPREIA
                     RVVRATRESVEERLRAARGEARQFLKDPSALRFMRDDAARAGELRFAREGAAVLTTDE
                     LLTDLRRAIFDSREGDCPAAAQLEGLYGAESASVDTRTLAHVVCCPCCIDEVNRLLGL
                     PPLAGRFPVDMTERDERPRRKGGGGDGGDGDDGGDGPRPTSGGASEEDVLRCRERARD
                     AFEHRPQELCVAVNGRVLATQSAGASRCEQTLALPLAEGVEFIEVFSEQEVRLLFIDV
                     NEHLAGARQSLSASVALSDERTLAATLSFGDAWPTVQVVYEDPLLHAAAQANAANESA
                     RANDATHSPRVAGASDDSGARVMGEAAASIRPPRALKISEWLTKVSARLTGAGFRLRP
                     AALTAALAIVVVAALLLTRVSTPTASAAELLDRSAAAEDKLATDAALVLHRTYFVEES
                     PAAAGAAVTRLRVEVWQSAARALKLRRVLDRENRLVAGAWEREGGARRTVYRRGAGPA
                     ESAAAPDALTLLEAGEAWGLDLSARTFSALVGDDTGGVTVEEALGSYVLTYRGGADGN
                     DSDGGGRSLLSATLRLSKSDLRATGQTLVVRRGEARREYRFIEGGLERLPAGSVAPGV
                     FQPEPELSGAATAADASVGTSAGQGGAVSSSGGGAPARAVASPELEVEVAYLLNRIKA
                     DMGEQITWTRTTGGQLRVEALAETEGRKEEILRALGPVIDNPAVEVDVATVEERVRRE
                     RVREGGEESTSEIEVRTGRTAAHEDLRRHFSARLAGDERVEAEIKAFGARAMNRSRAA
                     LLHASALKRLVARFTPEQARALRPEAREKWLAMLGGHAQAIRREVRALDGELRLVFPP
                     GNTGGGAGREASNPAASAESLLRLAYGADEAVRSAFTVTAGGASPAAGIKSPQFWRSL
                     RDADRLAASIADAYQK"
     gene            complement(7640..8575)
                     /locus_tag="VF240_02020"
     CDS             complement(7640..8575)
                     /locus_tag="VF240_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX7174048.1"
                     /translation="MELFRALAVLCESPRPETARVAEALGFGRAPTPAEFTEVFVFQL
                     PPYASVYLGVEGMLGGEARDRVAGFWRAAFGEDVPPAEPDHLPLMLALYARLVEFEGE
                     ANDAARREGWRAARKAFLWEHLSSWLPCYLRKLLEVAPPPYRAWGEVLMAALAGEAGA
                     LGRPERLSLHLREATSAADPRAAPAEEFLRSLLAPARCGMILTSSDLARAARETNLST
                     RAGERLFVLRTLLGQDAAGLLGWLAAEAARWASQHRQDADTFGETALWWEARARATST
                     LLEELRQQAAEESRTNGQDLQDEQDEKIPNSKSQL"
     gene            complement(8671..>11568)
                     /locus_tag="VF240_02025"
     CDS             complement(8671..>11568)
                     /locus_tag="VF240_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7174049.1"
                     /translation="RLSAARAETEARGETFYPGPSRVHLAAFPPKERWDDWVELDSRA
                     WPQRKERRYMLVPTTCFNCEAACGLLAYIDKESLQVQKFEGNPEHPGSRGRNCAKGPA
                     THNQITDPDRILYPLKRAGRRGEGKWVQVGWDEALDDIAARIRKCITEKRPNEVMYHV
                     GRPGEDGFTERVLAAWGVDGHNSHTNVCSSNARAGYQYWMGLDRPSPDHANAKVIFLI
                     SSHLETGHYFNPHAQRIIDAKQQGAKLIVFDTRLSNTATHADYWVAPYPGSEAAIVLA
                     MANHLIQNGLYNREFVRRWWNWEEFMAVERPELETSFESFERVLRELYAEYTFEFAAR
                     ESGVDAAVIEEIAGVVATAGTRLSTHNWRSAGAGNSGGWQVARTLFMLNALTGAIATE
                     GGVYPNAWNKFVPKPIHTPPHPKTWNELTWPREYPLAMNELSFLLPHFLMEGRGRVEV
                     YFTRVYNPVWTNPDGFSWVEALTDEKLVGCYVALTPTWNETAYFADYVLPMGHGSERH
                     DIHSYETHDAQWLGFRQPVLRAARERLGETIKDTRSVNPGEVWEENEFWLELTWRIDP
                     DGSLGIRQFAESKARPGERLSVDEYYAHIFENSVPGLPERAAGEGLTPLEFMRRYGAF
                     EIRRKVGALYEEPVPEGELDDAREDLFGRVYTRTPKPASVNIVPIPTPDGDAEGRRLV
                     GISVDGQTLRGFPTPSGRLEFYSKTLSDWGWPEWATPVYIRSHVHPENLAADQTVLIS
                     TFRLPVQIHTRSANSKWLDEIAHTNPLWIHTSHAARLGVRTGDLVRVETEIGYFVVRA
                     WATEGIRPGIVACSHHMGRWKLSDAGQRQLMATVALDHEGDRWGLKRKKGVGPYESSD
                     ADTLRIWWTDVGVHQNLTFPVHPDPVSGQHCWHQAVRVRRAEAGDSYGDISVDAAKAR
                     AVYRRWLAETRPAETHSPDHTRRPYWLLRPLKPAREFYKLPDEQTEPVGAD"
BASE COUNT         1828 a         3822 c         4113 g         1805 t
ORIGIN      
        1 aggcacccac tgctcggtct gtgccgaggc cgcgggcgcg gtcgtgagta gtagcagaag
       61 ggagaggatg acaggcgtgc gagttcgcat gacgtttact ccttgaaaca acttcataac
      121 agatttctct tcagaacaga actttcaggg ccaactgcac ggagcgcggg ccgccgacct
      181 gatagagcgg ggagagtccg ccgttagcgc cgcccgcgcc gaggctgcgg gcgagcgtct
      241 gcgtgctcag accgaagcgc gggttggcga ggtcggcgac ggggtcgccg aagctcgggc
      301 ggttgaacag gttgaagacc tcagcgcgca gttggaggcg cgcgcgctct gagagccgaa
      361 actcgcgccg gagcgccaga tcgatttgcg agaagccaaa gccgcgcacg gcgttgcggc
      421 cgagcgtccc ctgccgccca aagacctgca cgaaggccgc gcggttcaga cgccgcccgc
      481 cgggcgcttc ggggtcgtcc acgtagatgg gcacgccgga gacgagatcg ggccggcgcg
      541 cttcgaccag ctcgttgatt accgtgcccg tccgcgtgat gacgttgatg gggacggcgg
      601 tgcgcgcgcg caggagcgtg tccacggccc agccgtggaa gaggcgaccc gctgtggtgg
      661 cgcggccgtc gaagggtcgc ggcaggttgt aggtgacggc cgccgtcaga gaatgccgca
      721 cgtcgaagtt cgaggggccg tgctcgccct cggggtcgct tgagccggtg aagaggaagc
      781 tcgacgagtc gtcggagtcg ttgtcggtcg aacgcgcgaa ggtgtatgag gcgtgcgcct
      841 gaagtccgcc ggacagacgg cggatgaact gtagttgcag cgagtcgtag cgcgaagagg
      901 agctgttgtc cgtcacgcgc acgaccgtga aacggggttc gggtcgcagc agcgcgcgct
      961 gacgcagcag actccggccc gacgctccga cgtaggccgc cgagacggac tgtgcagcgc
     1021 cgagcgggtg ctccaagccg gcgttccact ggaacgcgaa gggcaggcgc agagagggga
     1081 cgaaggcgta gatggtgccg aagggcggat tgaggttgac gggcggcggc acggcctcgg
     1141 cggggtcgag cgggaaggcg acgttcgaga gcactttctc tttcgcgaag gggaagacgc
     1201 tgccgaaagc ctgcgcggcc tgccccgtgc cgaggtcgta gaagatgccg acgccgccgc
     1261 gcagcaccgt gccgcgctcg ggcagaagac ggtaggcgac gccgacgcgc ggcgcgaagt
     1321 tcccgtagga agtttgccac agcggcgtgc cgcgcggcgc gaggtcgagg tcgagacgcg
     1381 gcgtgccggc ggtgctgccg atgacgaggc cgtcgtcggc cgtctccagc ccggtgagca
     1441 cgacggggtg gttgtcgttg ctttcgcgtg gcggcgggtt cagctcccag cgcaggccgt
     1501 aggtgatggt gagacgcggc gtgatgcgtg cggtgtcctg tgcgtaggcc gagaagtttg
     1561 tgaagacggg cgtgcgcggc cccgcgtcgg ctccgacgcg gacctcgaag gcgcgtcccg
     1621 tggagatagc cgcgagcacg ccgttactcg cggtgtcgcc gaagacgacc gactgcgagt
     1681 agcgcagggg gccgtagacg ggcgcgaggc ggcggaagtc ggcgcccatg cggaagtggt
     1741 ggcggccgcg gacgacggag aggttgccga cggagttgag ctggcgttgg aggttgttga
     1801 cgctgcggcc cgtgccgagg tttgagttgg tgccgccgcg caggatgaac tggaagcccg
     1861 actcgtcggg cgtggcgaag ggcgggaaca ggaacgagtc ggcgggcgca cgcgcgccgc
     1921 cgaagtcgtc gagcgcaagc gtggtctcgg cggcggcgcg gctccagttg aaacgcaggt
     1981 cgccgaggac gttcggcgcg ggagcgaagg tcgcgccggc ggtgagtgtc tgcgtgtcga
     2041 gacgcgtgcg gcccacggtg ttgaggctct gcccggtgaa gtcggggagg ctgacgccgc
     2101 cgcgctggac ggtgtcggag ggcgcgtagt tgtatcggcc gaagaacgtg aggcgattca
     2161 tgacgacgtg gtcgattctc acgctcgcgg catcgaagcg cgcgcggtcg gaataggtgg
     2221 cggccgcctc ggcgaagccg ttgccgagat cggcgccggt ggggacgggg aaggcgtcga
     2281 agaagggacg caggggcgcg ggggccgact gacgcgcggc cgtcgaaggc acgtccgtga
     2341 tggcgacctg tggcagacgc aggcgcaggc cctcgtagga ggcgaagaag aaggtacggt
     2401 cgcggcgcag ggggccgccc agcgtcgcgc cgaagtcgtg ctgtcgcaga cgtgcgcgcg
     2461 gcagatcgcg actgttggcg aaccagtcgt tggcgtcgag cgcttcgtgg cggacgtagt
     2521 cgaagacgga gccgtgccac tcgtttgtgc ccgagcgcgt gacgacggag atttgcgcgc
     2581 ccgacgtgcg gccgaactcg ggcgcgtagg aagaggtctg gatgcgaaac tcttcgaggg
     2641 cttcgacgga gacgaggttg ttggcgccgc cgaggacggt aagcgcggga aggctacccg
     2701 aagcggactg accgggcgcg gcgccggccg agacgccgat gttggccgag acgccgtcca
     2761 ccatgaagta gttggcgttg gcgcgttgtc cggccgccga gaactgcccc tgctcgctga
     2821 agctggcgtg cgtcagggtc gtgcccggcg tcagctcgaa gagcgtctga aagctgcggc
     2881 cgttcaaggg caggttctcg acgaacggtc ggttgactac ggccgacacc gacggcccct
     2941 cgcgcacgac gcccaagccc tccagattat cgacgaccgt cacgctctcg ccgatctcgc
     3001 ccacgcgcag cctgatgcgt agcgccaact ggtcgctcac gttgagcgta atcgcgcgca
     3061 cttcgaccgt ggtgaagccg tcgcgctgcg ccgtcaggtt gtagcggccg acgggcagca
     3121 gcggcacgac gtagacgcct tcgccgttcg tcgcggcgtg gcgctccagc gccgtgctca
     3181 aattcagcac cgtgatccgc acgccgggga caaccgcccc cgcctcgtcg atgaccgcgc
     3241 ccgtcagcgt cgccgtcgcc gactgcgcgc gcgccagcgt gccgagcagc atgatgagaa
     3301 gcgcagccgc cgcgagccgg gagcgcgact ggtgcttaaa gtttttctca cgcgagatca
     3361 ttgctgctca caactcgacg gcgacgcccg ccgccacgtt cagtgaatga cgatgccgat
     3421 gccgacgcgg aagttgtgtt gtgtctggct gccgagatgc gtcggattgt aatcgagctg
     3481 gacggcgcgg aagtcgagcc tctcgctcag acgaatgtcc agcccgccgc cgatggcccc
     3541 ggcgatgccc gtctccgact cgtccgtcag gcacgcgaat cctaatgcct caaggcacgc
     3601 gctattgtcg aaagtgacct tggcgtgtgc ggccccgacg agcaggtgcg cgaacggttt
     3661 ggcgcggccc cccttcgcat tatctttgag ctgcaccccg ccgaggaaat tgtagagcga
     3721 agccttggct cgcggactga aacaaacgcc gccggtcgtg gcgtcaacgc agatgccgaa
     3781 gtcggcatcc gcctcccgct tgaagtggcc ggacacgtcg agcttgagtc ccgcgtagcg
     3841 tgaaaggttg ccgaccacgg aggcgttgaa gccgttaaag cccgtgtgtt cttcgtcgcc
     3901 cgcgaacgcc agcccatcgc cggggccgcc cgtgtccacg cggttgtgcg agaagccgcc
     3961 gaagaactcg aacctgttgt gatcgtccga cgactgcgcc agcgccgaaa gcgcggggca
     4021 catgatgaga gctgccaaca aaataagttt ccgcatgtga gaacccctca tggagctatc
     4081 ggttttgtag gacaggcatt cctgcctgtc gtgagcgcgg agcgcgaata aacttcgcgc
     4141 gcacattcaa cttgatattc ggcgtatcac ctgttcgcgc ttgcgcgctc aagacaggca
     4201 agaatgcctg tcctacagca agacgaaagc ggcttacttc tgatacgcgt ccgcaatcga
     4261 cgccgccagc cggtcggcgt cgcgcagtga gcgccagaac tgcggcgact tgatcccggc
     4321 cgctggcgac gcgccgccgg ccgtgacggt gaaggccgag cgcaccgcct cgtccgcgcc
     4381 gtaggcaagc cgcagaaggc tctcggccga cgcggccggg ttcgacgcct cgcggcccgc
     4441 gccgccgccg gtatttcccg gcgggaagac gaggcgcaac tccccatcga gcgccctgac
     4501 ttcccgccgg atggcctgcg cgtggccgcc cagcatcgca agccatttct cacgcgcttc
     4561 gggcctgagc gcgcgcgcct gctcgggcgt gaagcgcgcc acgagccgct tgagggccga
     4621 ggcgtgcagc agcgccgcgc gcgaacgatt catcgcgcgc gcgccaaacg ccttgatctc
     4681 cgcctcgacg cgctcatcgc ccgcgaggcg cgcggagaag tgacgccgga ggtcttcgtg
     4741 tgcggccgtc cggcccgtcc tcacctcaat ctcgctcgtg ctctcttctc cgccctcacg
     4801 cacgcgctcg cgtcttacgc gctcctcgac cgtcgccacg tccacctcga cggccgggtt
     4861 gtcgataact gggccgaggg cgcgcaggat ttcctccttg cggccctcgg tctcggcgag
     4921 cgcctcgaca cgcagttgtc ctcccgtggt gcgcgtccag gttatctgct cgcccatgtc
     4981 ggccttgata cgattcagca agtaagcgac ttcaacttca agctccggcg acgccacggc
     5041 ccgcgcgggc gcgcccccgc ccgacgacga gaccgcgccg ccctgtccgg cgctcgtccc
     5101 aacactcgcg tccgccgccg tcgccgcgcc cgaaagctcc ggctccggct ggaacacgcc
     5161 gggcgcgacc gagccggccg gcaaacgctc aaggccgccc tcgatgaagc gatactcacg
     5221 ccgcgcttct ccgcgtctga cgacgagcgt ctgccccgtg gcgcgcagat cggacttgct
     5281 cagcctgagc gtcgccgaca ggagcgagcg tccgccgccg tcactgtcgt tgccgtccgc
     5341 gccaccgcga taagtgagga cgtaagagcc aagcgcctct tcgaccgtca cgccgcccgt
     5401 gtcatctccg acgagcgcgg agaacgtccg ggccgagagg tcgagacccc acgcctcgcc
     5461 cgcttcgagg agggtgagcg cgtcgggcgc ggcggccgac tcggccggcc ccgcgccgcg
     5521 acggtagacg gtgcggcgcg cgccgccctc tcgctcccac gcgccggcga cgagacgatt
     5581 ctcgcggtcg aggacgcgac gcagcttcag agcgcgcgcc gcgctctgcc acacctcgac
     5641 gcgcagacgc gtcacggcgg cgcccgccgc ggccggcgac tcctcgacga agtacgtgcg
     5701 gtgtaagacg agcgcggcgt ccgtcgcgag tttatcttca gccgccgccg agcggtcgag
     5761 cagttcggcg gcggaggccg tgggcgtgct cacgcgcgtc agcagcagcg ccgccacgac
     5821 cacgatggcg agcgccgccg tcagcgccgc cggccgcaga cggaagcccg cgcccgtgag
     5881 ccgcgccgaa acttttgtca gccactccga aatctttaac gcacgcggcg gacgaatcga
     5941 cgccgccgct tcacccatca cgcgcgcgcc ggagtcgtca gaagcgccgg ccacgcgcgg
     6001 cgagtgggtc gcgtcgttcg cgcgggcgct ttcgttcgcg gcgtttgcct gagcggccgc
     6061 gtgaagtaga gggtcttcgt agacgacctg cacggtcggc cacgcgtcgc cgaagctgag
     6121 cgtcgccgcg agcgtgcgct cgtcgctcag ggccacggac gcgctcaagc tttggcgtgc
     6181 gcccgcgagg tgctcgttca cgtcgatgaa gaggaggcgc acctcctgtt cgctgaagac
     6241 ttcgatgaac tcgacgccct cggcgagcgg caacgcgagc gtctgctcgc agcgcgacgc
     6301 gccggcgctc tgcgtggcga ggacgcggcc gttgacggcg acgcacaact cctgcggccg
     6361 gtgctcgaag gcgtcgcgcg cgcgttcgcg acagcgcaac acatcttcct cactcgcccc
     6421 gccggacgtg ggacgtgggc cgtcgccgcc atcatcgccg tcgccgccgt cgcccccccc
     6481 gcctttacgc ctcggccgct cgtcgcgctc cgtcatgtcc acggggaagc ggccggcgag
     6541 cggcggcagg ccgagcaggc ggttgacctc gtcgatgcag cacgggcaac agacgacgtg
     6601 cgccagcgtc cgcgtgtcaa cgctcgcgct ttcggcgccg taaagacctt cgagttgggc
     6661 ggccgcgggg cagtcgccct cgcgcgagtc gaagatggcg cggcgcaagt ctgtgagcag
     6721 ctcgtcggtc gtcagcacgg cggcgccctc gcgcgcgaag cgcagctcgc cggcgcgcgc
     6781 ggcgtcgtcg cgcatgaagc gcagggccga agggtctttg agaaactgac gggcctcgcc
     6841 gcgcgcggcg cgcaggcgct cctcgaccga ctcgcgcgtc gcgcgcacta cccgggcgat
     6901 ctcgcgcggg tagtagccgt ggaagaaacg cagggcgagg acgctgcccg ccttcgacgt
     6961 gtgcatgcgc gcgcaggcgt agcggcagca ggcgcgcagc tcgtcgtgga cgcgtgcaag
     7021 gtcgcgcggg tcggcggcgc gcgccgcccc agaggccgag tcgtagtcga tggcggggag
     7081 catgcgctcg cgcaggcgtg cggcgcggcg ggcctgcgag gtgtgtaggt tgcgtaacat
     7141 gacgaacagg tagccgtcca gattttcgac ctcacggagc ggaggccgcg cgagcgtgaa
     7201 gtggacgtag gcgtcgtgga cgaggtcttc ggcgcgggcg cggtcgccct ccgcgagacg
     7261 cagcgcccag ccgcgcatgg ccgcgtagcg cgcgaggaag atgtcctcgt ggcattcggc
     7321 cggcgctgat gaggcggcgg cggcgggggc gatgtccggc agacgctgca ccataacttc
     7381 tcgcttctac cttttgaaga cgcgcgccgg gaggcggaac ccacgcgggc ggggagattt
     7441 ttttacgcgg gcgcgtcggc gcgtggggcg cgtccggggc gggcgcgcgg cgggtaagtt
     7501 aacacgttcg cggcgtcgtc tccacggcgc acacacgccc ggagtccgcg acccgcgact
     7561 ctaaaaagat tcaagcaaga atggacagga tttacaggat caacaggatg aagaaagagc
     7621 agatgtcaga gtcgagattt cagagttgtg atttgctatt tggaatcttc tcatcctgtt
     7681 cgtcctgtaa atcctgtcca ttcgtcctac tttcttccgc agcctgttgc cgcaactctt
     7741 cgagcagtgt ggaggtcgcg cgcgcccgcg cctcccacca gagcgccgtc tcgccgaacg
     7801 tgtccgcgtc ctgccgatgc tgcgaggccc aacgcgcggc ttcggcggcg agccagccga
     7861 gcagaccggc ggcgtcctga ccgaggagcg ttcgcagcac gaacagacgc tcgccggcgc
     7921 gcgtcgaaag attcgtttcg cgcgccgcgc gcgctaagtc ggagctcgtc aaaatcatcc
     7981 cgcacctcgc gggcgcaagc agcgagcgca ggaactcttc ggccggcgcg gcgcgcgggt
     8041 cggccgcgct cgttgcctcg cgcaagtgaa gcgacagacg ctcgggtcgg ccgagcgcgc
     8101 cggcctcccc ggcgagtgcg gccataagca cctcgcccca cgcacggtac ggcggcggcg
     8161 cgacctccaa gagcttccgc aggtagcacg gcagccagct cgacaggtgc tcccacagaa
     8221 acgccttgcg cgccgcgcgc caaccctcgc gtcgggccgc gtcgttggcc tcaccctcaa
     8281 actccacgag ccgggcgtag agcgcgagca tcagcggcag gtggtcaggc tcggcgggcg
     8341 gcacgtcctc cccaaacgcc gcgcgccaga atccggccac gcggtcgcgc gcctcgcccc
     8401 cgagcatccc ctcgaccccg agatagaccg acgcatacgg cggaagctgg aagacgaaga
     8461 cctccgtgaa ctcggcgggc gtcggcgcgc gcccgaagcc caacgcctcc gcgacgcgcg
     8521 ccgtctccgg tcgcggcgac tcacagagga cggcgagggc gcgaaagagt tccataagct
     8581 tcgcgtgata agtgatgagt gataagtgat aagaaagagc agacggcttt tcttatcact
     8641 tatcactcat cacttatcac taattccgtt ctagtcggcc ccgacaggct cggtctgttc
     8701 gtcgggcaat ttgtaaaact cgcgtgcggg tttgaggggg cggagcagcc agtaggggcg
     8761 gcgcgtgtgg tcgggcgagt gggtttcggc ggggcgcgtc tcggcgagcc agcggcggta
     8821 gaccgcgcgc gccttggcgg cgtccaccga gatgtcgccg taggagtcgc cggcttcggc
     8881 gcggcggacg cgcacggcct gatgccagca gtgctgaccg gagacggggt cggggtggac
     8941 ggggaaggtc aggttctggt ggacgcccac gtccgtccac cagattcgta gcgtgtcggc
     9001 gtcggacgac tcgtatgggc cgaccccctt ctttcgcttg aggccccagc ggtcgccttc
     9061 gtggtcgagt gcgacggtcg ccatcaactg ccgctggccc gcgtcggaga gtttccagcg
     9121 ccccatgtgg tgactgcacg cgacgatgcc ggggcggatg ccttcggtcg cccacgcgcg
     9181 gacgacgaag tagccgatct cggtctcgac gcggacgagg tcgccggtgc ggacgcccaa
     9241 acgcgcggcg tgcgacgtgt gaatccagag cgggttggtg tgggcgatct cgtcgagcca
     9301 cttggagttg gcgctgcgcg tgtgaatctg cacgggcagg cggaaggtgg agatgagcac
     9361 ggtctggtcg gccgcgagat tttcggggtg gacgtggctg cggatgtaga cgggcgtggc
     9421 ccactcgggc cagccccagt cggagagcgt cttcgagtag aactccagac ggccgctcgg
     9481 cgtcgggaag ccgcgaaggg tttgaccatc gacgctgatg ccgacgaggc gacggccctc
     9541 cgcgtcgccg tctggcgtcg ggatgggaac gatgttgact gatgcgggct tcggcgtgcg
     9601 cgtgtagacg cggccgaaga ggtcttcgcg cgcgtcgtcc agctcacctt ccggcaccgg
     9661 ctcctcgtag agcgcgccga ccttccggcg aatctcgaaa gcgccgtagc ggcgcatgaa
     9721 ctcaagcggc gtcaagccct ctcccgcggc gcgctccggc agacccggca ccgagttctc
     9781 gaagatgtgc gcgtaatact catcgacgct cagtcgctcg ccgggacgcg ctttcgactc
     9841 ggcgaactgg cgtatcccta aactcccatc ggggtcgatg cgccacgtca gttccagcca
     9901 gaactcgttc tcctcccaga cctcgccggg gttgacgctg cgcgtgtcct tgatggtctc
     9961 gccgaggcgc tcgcgcgcgg cgcgcagcac gggctggcgg aagccgagcc actgcgcgtc
    10021 gtgcgtctcg taggaatgta tgtcgtggcg ctcgctgccg tggcccatcg gcaggacgta
    10081 gtcggcgaag taggcggtct cgttccacgt cggggtgagg gcgacgtagc agccgacgag
    10141 tttttcgtcc gtcagcgcct cgacccacga gaagccgtcg gggttcgtcc acacgggatt
    10201 gtagacgcgc gtgaagtaga cctcgacgcg gccacggccc tccatcaaaa agtgcggcag
    10261 caggaaagac agctcgttca tcgcgagcgg gtactcgcgc ggccacgtca gctcgttcca
    10321 cgtcttcggg tgcggcggcg tgtgtatggg cttcggcaca aacttattcc aagcgttcgg
    10381 gtagacgccg ccctcggtgg cgatggcgcc ggtgagcgcg ttgagcatga agagcgtgcg
    10441 cgccacctgc cagccgccgc tgtttcccgc gccagcgctg cgccagttgt gagtcgaaag
    10501 acgcgtgccc gcggtcgcca cgaccccggc gatctcctcg atgacggcgg cgtccacgcc
    10561 cgactctctg gccgcgaact cgaacgtgta ctccgcgtac aattccctga gcacgcgctc
    10621 gaagctctcg aacgacgttt ccagctccgg ccgctccacg gccatgaact cctcccagtt
    10681 ccaccagcgg cgcacgaact cgcggttgta gagtccgttc tgaatgagat ggttcgccat
    10741 tgcgagcaca atcgccgcct cggagccggg gtagggcgcg acccagtagt cggcgtgcgt
    10801 cgccgtgttg gagagacgcg tgtcgaagac gatgagcttc gcgccctgct gcttggcgtc
    10861 gatgatgcgc tgcgcgtgcg ggttgaagta gtggccggtc tccagatgtg agctgatgag
    10921 aaaaataact ttcgcgttcg cgtggtcggg actcggccgg tcgaggccca tccagtactg
    10981 gtagccggcg cgcgcgttcg acgagcagac gttcgtgtgc gagttgtgcc cgtccacgcc
    11041 ccacgccgcg agcacgcgct cggtgaaccc gtcctcgccg gggcggccca cgtgatacat
    11101 cacctcgttc ggccgcttct ccgtgatgca cttgcgaatg cgcgcggcga tgtcgtccaa
    11161 cgcctcgtcc caaccgacct gcacccactt cccttcgccg cgccgccccg cgcgcttcaa
    11221 gggatagaga atccggtcgg ggtcggtgat ctggttatgc gtcgccggcc ccttggcgca
    11281 gttgcgcccg cgcgagccgg ggtgctcggg gttcccctcg aacttctgca cctgcaagct
    11341 ctctttgtcg atgtaagcca gcagcccgca cgccgcctcg cagttgaagc acgtcgtcgg
    11401 cacgagcatg taccggcgct ccttccgctg cggccacgcc cgcgaatcca actccaccca
    11461 gtcgtcccac cgctccttgg gcgggaacgc cgcgaggtgc acgcggctgg ggccgggata
    11521 aaaggtctcg ccgcgcgcct cggtctcggc gcgcgccgcg gagaggcg
//
LOCUS       DASVES010000053         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1308055, whole genome shotgun sequence.
ACCESSION   DASVES010000053 DASVES010000000
VERSION     DASVES010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308055"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VF240_02030"
     CDS             <1..657
                     /locus_tag="VF240_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7174050.1"
                     /translation="TQLIAGNVATGDATRELISAGVDAVKCGIGPGSICTTRVVTGAG
                     VPQITAITNCVQAARGSGVPVIADGGVKFSGDVAKAMAAGADVVMIGSLFAGTEESPG
                     ELILFQGRSFKAYRGMGSIGAMRQGSKDRYAQEETDVEAKLVPEGIEGRVPYKGTLAD
                     MVTQLVGGLRAGMGYTGCRTVREFQERTQFVRITSAGLKESHVHDVIITKEAPNYRLE
                     "
     gene            862..1758
                     /locus_tag="VF240_02035"
     CDS             862..1758
                     /locus_tag="VF240_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7174051.1"
                     /translation="MTERTALLISNPNAGRGGLVREREVGRFREELERRGVHVELLST
                     AGPRDAERLAREGVARGLREVIVAGGDGTVNEALQGLVGSGARVGVWPAGTANVLARH
                     LGLPFDAEGAARAVASGRTRRITLGRAVEEESGRSRFFFLMAGVGLDASVVKNVRPRL
                     KKRVGEAAYWFSGFAHLAHWRPRIFTVEVEGREYPATYAAVGKAPWYGGGLSLTPRAR
                     LDAGEFEVCLINTRSRLRYLRLLSHAVRGGAPEGTRGVTYLRARRLRATGDALVQTDG
                     ELTGRLPMTFEVVTEGVEIVVP"
     gene            complement(1778..2023)
                     /locus_tag="VF240_02040"
     CDS             complement(1778..2023)
                     /locus_tag="VF240_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174052.1"
                     /translation="MYGAIKKLFVAACAFAAGSLACFYAGAQRAAGVAPALNPDDLSR
                     QFTTDPGEGLVLAGGFLLVVALALASAGLMLWRQERS"
     gene            complement(2233..>2638)
                     /locus_tag="VF240_02045"
     CDS             complement(2233..>2638)
                     /locus_tag="VF240_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7174053.1"
                     /translation="TPAKQPHTAGIGFDFRAHPLLRVGYLSTGSAAQRAGLRQGDVLV
                     TIGGREVTIENWPEALGAFKTGDRVPINVRRGRETIAATLTLGEPDVYEYNIEFVENA
                     PAEKRALRDAWLSGAGPWRRAPDKPPVRRGNN"
BASE COUNT          388 a          823 c          987 g          440 t
ORIGIN      
        1 acgcagctca tcgccggcaa cgtcgccacg ggtgatgcga cgcgcgaact catcagcgcg
       61 ggcgtggacg ccgtcaagtg cggcatcggg ccggggtcga tttgcacgac gcgcgtcgtg
      121 acgggcgcgg gcgtgccgca gattacggcc atcacgaact gcgtgcaggc ggcgcgcggc
      181 tcgggcgtgc ccgtcatcgc cgacggcggg gtgaagttct cgggcgacgt ggcgaaggcg
      241 atggcggccg gcgcggacgt ggtgatgatc ggctcgcttt ttgccgggac ggaggagtcg
      301 ccgggcgagt tgattctctt tcaggggcgc tccttcaagg cgtatcgcgg gatgggttca
      361 atcggcgcga tgcggcaggg gtcgaaggat cgctacgcgc aggaggagac ggacgtggag
      421 gccaagctcg tgcctgaagg catcgagggc cgcgtccctt acaagggcac gctcgccgac
      481 atggtgacgc aactcgtcgg cggcctgcgc gccggcatgg gctacacggg ctgccgcacg
      541 gtgcgtgagt ttcaggaacg gacgcagttt gtccgcatca cctccgccgg cctcaaggag
      601 tcgcacgtcc acgacgtgat tatcacgaag gaagcgccga actaccgtct ggagtagacg
      661 gctcggagtt tccggctacg tgggcgcggg gagctttcgg gcttcgtccg cgccctcttg
      721 gtttatgagg cgtgcggcag ggcgcgcccg acacgtgtcc gggaaacaat gcacgcgcga
      781 ccgggcggag tttcgcgccc cgcggtttcg acgggaaaac tccggcgcga gggtggcgcc
      841 gggcttgcca tgaatcagac gatgacagag cgcacagcac ttctcatttc gaacccgaac
      901 gcgggacgcg gcggtctcgt tcgtgagcgc gaggtcgggc gtttccgcga ggagttggag
      961 aggcggggcg tccacgttga gctgttgagc acggcggggc cgagggacgc ggagaggctg
     1021 gcgcgcgaag gcgtcgcgcg gggtctgcgc gaggtgatcg tcgcgggggg cgacgggacg
     1081 gtgaatgagg ctttgcaggg attggtcggg tcgggcgcgc gcgtcggcgt gtggccggcg
     1141 gggacggcga acgtgctggc gcgacacctg gggctgccct tcgacgccga aggggccgcg
     1201 cgcgccgtgg cctccgggcg cacgcggcgc atcacgctcg gccgcgccgt tgaggaggag
     1261 tcggggagga gccgcttctt cttcctgatg gcgggcgtcg ggctggatgc atcggtcgtg
     1321 aagaacgtgc gtccgcgtct gaagaagcgt gtgggcgagg cggcctactg gttttcgggc
     1381 ttcgcacatc tcgcccactg gcgcccgcgc atcttcactg tcgaggtcga gggccgcgag
     1441 tacccggcca cctacgcggc cgtcggcaag gcgccgtggt acggcggcgg cctgagtctc
     1501 acgccgcgcg cgcggctcga cgccggcgag tttgaagtct gcctcatcaa tacacgtagc
     1561 cgactgcgct acctccgtct gctctcgcac gccgtgcgcg gcggcgcgcc cgagggcacg
     1621 cgcggcgtca cctatctgcg cgcgcggcgt ttgcgcgcca ctggcgacgc gctcgtgcag
     1681 accgacggcg aattgacggg acggctgccg atgaccttcg aggtcgtcac cgagggcgtt
     1741 gagatcgtcg tcccttaaac gacgccggcg ccggctctca actccgctcc tgccgccaca
     1801 gcatcaggcc cgcggaggcg agggcgagcg cgacgacgag gaggaagccg ccggcgagca
     1861 cgagtccttc gccggggtcg gttgtgaact gccggctcag gtcgtcgggg ttgagcgcgg
     1921 gcgcgacgcc ggccgccctc tgtgccccgg cgtagaaaca ggcgagcgaa ccggccgcga
     1981 acgcacacgc cgcgacgaag agttttttga tagccccgta catcttccga aacccttctt
     2041 tctccggtgc cgatacgtgt agacaccggc gtgtggacga aggttccggc cgcggcgacg
     2101 ggttatgata gacctgtgcg ggtgggtggg tgcgtgttgt cgggcagagg gaacacggat
     2161 taaacagata agacggatga agacggattt catccgtctt catccgtctt atccgtctca
     2221 tccgtgttcc gcttaattat tcccgcgcct cacgggcggc ttatccggcg cgcgccgcca
     2281 cggccccgcg ccgctcagcc acgcgtcgcg cagggcgcgc ttctctgccg gcgcgttctc
     2341 cacaaactcg atgttgtact cgtaaacgtc cggctcgccg agtgtgagcg tcgccgcgat
     2401 ggtctcgcgt ccacgccgca cgttgatggg cacgcgatcc ccggtcttga atgcgccgag
     2461 agcttccggc cagttttcga tggtcacttc ccggccgccg atggtgacga gcacgtcgcc
     2521 ctgtcggagt ccggcgcgct gggccgcgga gccggtgctg agatagccga cgcgcagtag
     2581 cgggtgggcg cggaagtcga agccgattcc ggccgtgtgc ggctgcttcg cgggcgtg
//
LOCUS       DASVES010000054         6799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_917596, whole genome shotgun sequence.
ACCESSION   DASVES010000054 DASVES010000000
VERSION     DASVES010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917596"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF240_02050"
     CDS             <1..180
                     /locus_tag="VF240_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174054.1"
                     /translation="EGGEGGGRVVAAGTPEDVARSRRSYTGQALRPLLNGHVVNGNGH
                     ARAKTQSAPTRKKKA"
     gene            421..1110
                     /locus_tag="VF240_02055"
     CDS             421..1110
                     /locus_tag="VF240_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174055.1"
                     /translation="MASDNDAAEMNGVARTGFYTPSRLLREDESAGQGGAQQAQQQPQ
                     PPAQTYTPLTPDGKMKRAFRRAFLSPEGYIRSAVAATLTEWGEDDLPHKTTEDRLADG
                     LSRFAIKFSTRATRTLLGSGVYPVIFKQDPRYERSRSKNVGRRALHAASRVFVTRGDN
                     GRLQPNYSRWAGSLSASALSNLWEQSTPGHDRIGADATFRRFGTSFITDAWQTAVFGE
                     FLPDLIKIFKR"
     gene            complement(1378..1860)
                     /locus_tag="VF240_02060"
                     /pseudo
     CDS             complement(1378..1860)
                     /locus_tag="VF240_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(1873..2187)
                     /locus_tag="VF240_02065"
     CDS             complement(1873..2187)
                     /locus_tag="VF240_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174056.1"
                     /translation="MRKAAAAFALAVSVLACAQGVGAQQRQRRPSQTDAELRALREEI
                     DALRQGQLEIQSELRELKELMQQLAEASAQGGRKLVSVDDDPSMGETTAKVVLIDFSD
                     YQ"
     gene            2384..2965
                     /locus_tag="VF240_02070"
     CDS             2384..2965
                     /locus_tag="VF240_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEX7174057.1"
                     /translation="MSETIKSIVAESLEVKRRYFDEHAADVTRAARLLAEAFASGRKL
                     LICGNGGSAADAQHVAGEFVNQFLARGRRALPALALTTDGGVLTCIANDTGFERVFAR
                     QVEAFGGEGDVLLAITTSGNSPNIVAAAEVARARGMKVIGLLGRDGGRVRPLCDLALV
                     VAADDTQRVQETHNLIGHVLCQLVENILFPAGE"
     gene            3116..4078
                     /locus_tag="VF240_02075"
     CDS             3116..4078
                     /locus_tag="VF240_02075"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174058.1"
                     /translation="MSIYDERGIRPLGLEGVSTYPLSERPSKVSTEDFARPVAADSSL
                     KDFLSTLPNILAVQSLRQIAAQVRRARELGKPIVWGLGGHVIKTGLAPLLIDLMRRGY
                     VSAVAANGSVLVHDAEIALVGSTSEDVDSTLGAGAFGGADETGKLLNSAARDGARDQL
                     GLGEALGRALTAARPKHADLSLLCAAYDARVPFTAHVAIGTDITHFHPSADGAALGAA
                     THTDFRLLSEMVRRMSGGGVYLNVGSAVVLPEVFLKAVTLVRNLGHPLADFTTANFDF
                     IQSYRPLTNVVRRPVSGGAGRGYAVTGHHELTIPLLAAHILCGD"
     gene            4326..>6799
                     /locus_tag="VF240_02080"
     CDS             4326..>6799
                     /locus_tag="VF240_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7174059.1"
                     /translation="MQKNLLQAAASARLAASPIVLLALLCALATNVAAQSLRSEISGH
                     VLDQNKAAVAGARVTLQRAGTGFELSTATDDAGAFSFGRLAAAEYSVVASSAGFASSR
                     REVVLKSDERRQLDLVLQAGPVAEAIVVSSSHIAGTPEVVERIPGSVEVLDERLLESS
                     RVFNTSEALRKSSGVNVRDEEGFGLRPNIGIRGLNPTRSSKVLLLEDGIPFTYAPYGD
                     NASYYHPPIDRFESVEVLKGSGQILFGPSTVGGVINYVTRNPPVKRAGTLTLVGGGRD
                     YFNGHLSYGGTWGNTGLLFDYTRKQGEGARADQRSGLNDFNFKSVTTLGARQALTAKF
                     NYYGEDSQVTYSGLTEAEYAAAPRQNPFRNDHFYGDRYGASLSHAFIFSGDVVLTTNL
                     YGSHFRRHWWRQSSNSLERPNRLATLAGGDSDCRSLADLDTTCGNQGRLREYYFFGVE
                     PRLHVNHRLFGVRSEADFGFRAHYETQQRRQENGDLPTSRSGLLVEHNERRNQAYSGF
                     VENRFHFGRWSVTPGLRVEHVEYQRTNRLANGGLGVSGRTDITQAVPGLGVSYTPGAR
                     FTLFAGVHRGFAPPRTEDVINNATGGVIELDPELSWNYELGARTAPARGLRLDATFFR
                     MDYENQIVPASLAGGVGATLTNGGETLHQGFEFTGRLDSGVIRESPHNFYVRAAYTFL
                     PTAKFTGVRLSSITNADGTRPAITGNRLPYAPEHLLNATFGYAHPSGFDGLLEAVHVG
                     AQFADDRNFREVPAGVVSESLRRSGQLGLIPGYTAWNATVNYRVEHLRTTFFVTVKNL
                     FDRTYVVDRTRGLLPSSPRLLQGGLK"
BASE COUNT         1155 a         2500 c         1954 g         1190 t
ORIGIN      
        1 gagggcggcg agggcggcgg ccgcgtcgtc gccgccggca cccccgaaga tgtcgcccgc
       61 tcccgccgct cctacaccgg ccaggctctg cgccccctcc tcaacggtca cgtcgtgaat
      121 ggcaacggcc acgctcgcgc caaaactcag tccgccccca cgcgtaagaa aaaggcttga
      181 aatttaagcc cacacgcggc tagcatggtc tgacggattg tcatcgcttt taaatcgagc
      241 ctgcggtttt ggaaggcgcg cgtggccgcc cgcctttcag aagggaaact tatgtcacaa
      301 ggacagctat tgaaacattc tctggcgtgt cttctcgtac tcgccgccat ttcgctcaac
      361 tctcacgccg ttagctctca aacactatcg tcaaccgcga cgccgcccgc gacgacgtta
      421 gtggcctctg acaacgacgc cgccgagatg aatggcgtgg ctcgtaccgg gttttacacg
      481 ccgtcccgtt tgctcaggga agacgagagc gcagggcagg gcggcgcgca gcaggcgcag
      541 cagcaaccgc agccgcccgc gcagacttac acgcccctca cccccgatgg aaagatgaaa
      601 cgcgctttcc gaagggcgtt cctcagcccg gagggttaca tcaggtcggc cgtcgccgcc
      661 accctcaccg agtggggcga ggacgacctg ccgcacaaga ccacggaaga ccgtcttgcc
      721 gacggcctct cgcgcttcgc catcaagttc tcgacacgcg ccacgcggac tctgctcggc
      781 tcgggcgtct accccgtcat cttcaaacaa gacccgcgct acgaacgctc gcggagtaag
      841 aacgtcggcc gccgcgccct ccacgccgcg tcgcgcgtct tcgtcacgcg cggcgacaac
      901 ggacgcctcc agccgaacta ctcgcgctgg gccggcagcc tctcggcgag cgcgctctcg
      961 aacctctggg aacagagcac gcccggccac gaccgcatcg gcgccgacgc caccttccga
     1021 cgcttcggca cgtcgttcat caccgacgcc tggcagaccg ccgtcttcgg cgagttcctg
     1081 cccgacctca tcaaaatctt caagaggtag ccgacccttc aagctcgcca gcgccgaagg
     1141 cgcggcagaa tgtagcccac ggctcgcgcc gtgggaacag gcggatttaa aagtcgaagc
     1201 cccggagggg cgaaagaaaa gcgacgttga ttattctcaa cgtcggtttt ctttcgcccc
     1261 tccggggctt tcatgttttt tctaaaccgc cgaccctggt ctcacgaccg gggcaacact
     1321 gctgccgccg ctccgcggct cgttgtcgtt tccccttcga gttgacctgt cgtgggccta
     1381 ctgcttctcg gcctcggcca gcgcgtcgtc aagcatcgcc ttgaaggcgg cgtatgattg
     1441 cgcgccggtg aaggatcgaa agatgcggac gttcgggtcg cgcggattct tcgggttgac
     1501 gatcccgatg atgaagcccg gcgtgccgcc gacctgcgcc gccgccgcgt cgctcatgtc
     1561 cttccgcacc tcggccgcgt gctttccgct ctccagacat tgcgcgaact tcgcgccgtc
     1621 aagccccaac gcctgcgcgt actgtgtgag gttggccggc gccagcgccc gcgtgttctc
     1681 aaagaggcgg ttgtgcatct cccagaactt cccctgctcg ccggcgcagt tggccgcctc
     1741 ggcggccttg aaggcgtgcg gatgaatctg cacgagcggc aggtcgcgga agacataacg
     1801 caccttgccg gtcttgatgt actcctgcac gatctgtggc ttcgtctcac ggacaaagcg
     1861 agcgcagaac ggtcattggt agtcggagaa gtcgatgagg acgactttgg ccgtcgtctc
     1921 gcccatcgac gggtcgtcgt ccacgctgac gagtttgcgc ccgccctgcg ccgacgcctc
     1981 ggccaactgc tgcatgagtt ctttcaactc gcgcagctcg ctttgaatct ccaactgccc
     2041 ctgcctgagc gcgtcaatct cctcgcgcaa cgcgcgcagc tccgcgtccg tctggctcgg
     2101 acgcctctgc cgctgctgtg cgcccacacc ttgcgcgcac gcgagcacac tgacggctaa
     2161 ggcaaatgct gccgccgctt ttctcattcc actctcctcc gatgatgatt cgcgcgaaaa
     2221 agccgcgcgt gatgaaagca acctcaagat acggcagccg cgcccggcgg cgcaacacgg
     2281 ctcgctcccg ccgccagcgg cgacgacttc gcccatgcct tcgatacttg tcgcgccgtt
     2341 gatgtatact cgacccccgc cgaagagcac actgaaagag ataatgtcgg aaaccatcaa
     2401 atcaatcgtc gccgagagcc ttgaggtcaa gcgtcgctac ttcgacgagc acgctgccga
     2461 cgtgacgcgg gccgcgcgcc ttttggccga ggcgttcgcc tcggggcgca agctcctcat
     2521 ctgcggcaac ggcggctccg cggccgacgc gcaacacgtc gccggcgagt tcgtcaacca
     2581 gttcctcgcc aggggccgcc gcgcgctccc cgccctcgcg ctcaccaccg acggcggcgt
     2641 gctgacctgc atcgccaacg acaccggctt cgagcgcgtc ttcgcccgac aggtcgaggc
     2701 gttcggcggc gagggcgatg tgctgctggc gattaccact tcgggcaact cgcccaacat
     2761 cgtcgccgcg gccgaggtcg cgcgagcgcg cggcatgaag gtcatcggcc tgctcggacg
     2821 cgacggcgga cgcgttcgtc cgctctgcga cctcgcactc gtcgtcgcgg cagacgacac
     2881 acagcgcgtg caggagacgc acaatctcat cggccacgtc ctctgccaac tcgtcgagaa
     2941 catcctcttc cccgcaggcg aatgattatg accgggaaga attaacatgg atggacaaga
     3001 taggtaggat gaagaaagac tgatttgaga tttgagattt caaatattcc tatcctcctc
     3061 atcctgccga tccatgttaa tttctttaac tcgtgaaaca ggagcgttga gttaggtgtc
     3121 catctacgac gaacgcggta tccggccgct cggcctcgaa ggcgtgagca cctacccgct
     3181 ctcggagcgc ccgagcaagg tctcgaccga agacttcgcg cggcccgtcg ccgccgactc
     3241 ctcgctcaaa gattttctct cgacgctccc gaacatcctc gccgtccaat cgctcagaca
     3301 gatcgccgcg caggtgcgcc gcgcacgcga actcggcaag cccatcgtct ggggtctcgg
     3361 cggccacgtc atcaagaccg gcctcgcgcc gctcctcatc gacctcatgc gccgcggcta
     3421 cgtgtcggcc gtcgccgcca acggctccgt gctcgtccac gacgccgaga tcgccctcgt
     3481 cggctcgacc tccgaggacg ttgactccac gctcggcgcg ggcgctttcg gcggggccga
     3541 cgagactgga aaactcctga actcggccgc ccgcgacggc gcgcgcgacc agttgggtct
     3601 gggcgaggct ctgggtcgcg ccctcaccgc cgcgcgtccg aagcacgccg acctctcgct
     3661 gctctgcgcc gcctacgacg cgcgcgtgcc cttcaccgca cacgtcgcca tcggcacaga
     3721 catcactcac ttccacccgt cggccgacgg cgccgcgctc ggcgccgcca cacacacgga
     3781 cttccgcctg ctctccgaga tggtgcgtcg gatgagcggc ggcggcgtct acctcaacgt
     3841 cggctcggcc gtcgtgctcc ccgaagtctt cctcaaggcc gtgacgctcg tgcgcaacct
     3901 cggccacccg ctcgcagact tcaccaccgc caacttcgac ttcatccaat cctaccgccc
     3961 gctcaccaac gtcgtccgcc gccccgtgag cggcggcgcg ggccgcggct acgccgtcac
     4021 cggccaccac gaactcacca tccccctcct cgccgcacac atcctctgcg gcgactgaca
     4081 aactactcag ccctgcggcg taccctctgc gcccgccgtg cctgcggcgg tgaatccact
     4141 gccgctttca ctcaagtgga acatcgctcc agcgactaca cgtccggtgc gaagccccct
     4201 cttgacacat tcaaccggga gacttattct tcccctgtct gaaattgaaa atcaatttca
     4261 aatcttctca gattattagt ttcgggctgt tatttgagct tcttcggctt cccggtaggt
     4321 aactcatgca aaagaatctg ctacaggcgg cagcctcggc ccgcctcgcc gcgtccccga
     4381 ttgtcctgtt ggcgctgctc tgcgccctcg ccacgaacgt cgcggcgcag tctttacgct
     4441 cggaaatttc cggccacgtg ctcgatcaga acaaggccgc ggtcgccggc gcacgcgtca
     4501 ccttgcagcg cgcagggact ggcttcgagc tgagcaccgc gaccgacgac gccggcgctt
     4561 tcagcttcgg ccgacttgcc gcggccgagt acagcgtcgt cgcttcgagc gccggcttcg
     4621 cgtcgtcgcg gcgggaggtc gtgctcaagt ctgacgagcg ccgccagctc gatctcgtcc
     4681 ttcaggccgg ccccgtcgcc gaggcgattg tcgtctcctc ttcccacatc gccggcacgc
     4741 cggaagtggt cgagcgcatc cccggctccg tcgaggtgct cgacgagaga ctcttggagt
     4801 cgagccgggt cttcaacacc tcggaggcgc tgcgcaagtc ttcgggcgtc aacgtccgcg
     4861 acgaggaggg cttcggcctg cgcccgaaca tcggcatccg cggcctcaac cccacgcgct
     4921 cctcgaaggt gctgcttttg gaagacggaa tccccttcac ctatgcgccc tacggcgaca
     4981 acgccagcta ctaccacccg cccatcgacc gcttcgagtc cgtcgaagtg ctcaaggggt
     5041 cggggcagat tctcttcggc ccctcgacgg tcggcggcgt catcaactac gtcacgcgca
     5101 acccgcccgt gaagagagcc ggcacgctga cgctcgtggg cggcggccgc gactacttca
     5161 acggccacct ctcctacggc ggcacgtggg gcaacaccgg cctcctcttc gactacacgc
     5221 gcaagcaggg tgagggcgcg cgcgccgacc agcgttcggg cctcaacgac ttcaacttca
     5281 agtctgtaac gaccctcggc gcgcgtcagg cgctcaccgc caaattcaac tactacggtg
     5341 aggactcaca ggtcacttac tcgggtctga ccgaggccga gtacgccgcc gcgccgcgcc
     5401 agaatccttt ccgcaacgat cacttctacg gcgaccgcta cggcgcctcc ctttcgcacg
     5461 cttttatctt ctccggcgac gtggtgctca ccaccaatct ctacggctca cacttccgac
     5521 gccactggtg gcgccagtcg tcgaactcgc tggagcgccc gaaccgtctc gcaacgcttg
     5581 cgggcggcga ctctgactgt cgcagcctcg ccgacctcga caccacctgc ggcaatcagg
     5641 gacgcctgcg cgaatactac ttcttcggcg tcgagccgcg cctgcacgtc aaccaccgcc
     5701 tcttcggcgt ccgcagtgag gccgacttcg gtttccgcgc gcactacgag acgcagcagc
     5761 gccggcagga gaacggcgac ctgccgacct cgcgctccgg cctcctcgtc gagcacaacg
     5821 agcgacgcaa tcaggcctac tccggcttcg tcgagaaccg cttccacttc ggccgttggt
     5881 cggtgacgcc gggcctgcgc gtcgagcacg tcgagtatca gcgcacgaac cgtctcgcta
     5941 acggcggcct cggcgtgagc ggccgcacgg atattacgca ggccgtcccc ggtctcggcg
     6001 tctcctacac gccgggcgcg cgcttcaccc tgttcgccgg cgtccaccgc ggcttcgccc
     6061 caccgcgcac cgaagatgtc atcaacaacg cgacgggcgg cgtcatcgaa ctcgaccccg
     6121 agctgagctg gaactacgaa ctcggcgcac gcaccgctcc ggcccgcggc ctgcgtctcg
     6181 acgccacctt cttccgcatg gattatgaga accagatcgt cccggcgagc ctcgcgggcg
     6241 gcgtcggcgc cacgctcacg aacggcggcg agacgctgca tcagggcttc gagttcacgg
     6301 gccgtctcga ctccggcgtg atacgagagt cgccgcacaa cttctacgtg cgcgcggcct
     6361 acaccttcct ccccacggca aagttcacgg gcgtgcgttt gagcagcatc acgaacgccg
     6421 acggcacgcg ccccgccatc accggcaacc gtctccctta cgcgcccgag cacctgctca
     6481 acgcgacctt cggctacgcg cacccgtccg gcttcgacgg cctgctcgaa gccgtccacg
     6541 tgggcgctca gttcgccgac gaccgcaact tccgtgaagt gccggccgga gtcgtctctg
     6601 aaagcctgcg ccggagcggg cagctcggcc tcatccccgg ctacacggca tggaacgcga
     6661 ccgtcaacta tcgcgtcgag cacctgcgaa cgaccttctt cgtcacggtc aagaatctct
     6721 tcgaccgcac ctacgtcgtt gaccgcacac gcggcctcct gccgagcagc ccgcggctct
     6781 tgcagggcgg attgaagtt
//
LOCUS       DASVES010000055         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1525125, whole genome shotgun sequence.
ACCESSION   DASVES010000055 DASVES010000000
VERSION     DASVES010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525125"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VF240_02085"
     CDS             complement(<1..848)
                     /locus_tag="VF240_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7174060.1"
                     /translation="MQLREAKGLQIAHSSEITRVENLWIVPSQTSAKKYAVDLDINPP
                     TCTCPDCKKNDRICKHIFAAQYAKEREGGAELPEAPKIEKRKYTQNWPQYHRAQVAEK
                     GKFQLLLAELCKGIEEPIQEYGRPRAVLADVIFAAAFKIFSTVSTRRFCSDLKEAHAK
                     GYVSKALSYNCVLEYLGYAELTPYLHQLIEVSSLPLKGVESDFAVDSSGFGTGQFTRW
                     FNAKYTKNGDSDMHDWLKAHIMVGVKTHIITSVEVSDRHDNDYGFFEPLVKSTAQNFK
                     MREVSA"
     gene            complement(858..1991)
                     /locus_tag="VF240_02090"
     CDS             complement(858..1991)
                     /locus_tag="VF240_02090"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX7174061.1"
                     /translation="MRLPSLKDALADVPDFRQAQGRRYDLLPVLLLCCVAVMCGARSQ
                     AAIADWGHNYGARWLSRLGLRRGRGPSQPTLHRIFKGLDCARLEQCVTRWAEHILAAC
                     AAPAAALEALAIDGKTLRGSAGQGAAESHLVSALSHRLGVVVAQLAVADKSHELGHLE
                     PLLDALVLDGRVVTADALHTQREVARTVVARGGDYLLPVKENQPVLRSDIALLFAHAK
                     ELADTIGHAQTTDQHGDRIEVRCLSASTALQGYVEWPGHAQVLELRRQVTNKRTAETR
                     HEIVYGITSLAPERATPAQLLRLWREHWHVENKLHYVRDVTFDEDRSQVRAGHAPQVM
                     AALRNVAISALRLCGAENIAAACRRYAARPALALAAVGIRLRE"
BASE COUNT          389 a          677 c          709 g          447 t
ORIGIN      
        1 tctgcgctga cctcgcgcat cttgaagttc tgcgccgtgg acttaaccag cggctcaaag
       61 aatccgtaat cgttatcgtg ccggtctgag acttcgacgc tcgtgatgat gtgagtcttc
      121 accccgacca tgatgtgagc cttgagccag tcgtgcatat cggagtcgcc gttcttcgtg
      181 tacttggcat tgaaccagcg ggtgaactgc cccgtgccga agcccgacga gtcaacggcg
      241 aagtctgatt cgacgccctt gagcggcagg ctcgacactt cgattagctg atgcaggtac
      301 ggcgttagtt ccgcatagcc taagtattcg agcacgcagt tataagagag cgccttcgag
      361 acgtagcctt tggcgtgcgc ttctttcaag tcgctgcaaa agcggcgcgt gctgacggtc
      421 gagaaaatct tgaacgcggc ggcgaagatc acatccgcga gtaccgcgcg aggtcgcccg
      481 tactcctgta tcggttcttc gatgccttta cacaactcgg caagcaggag ttggaacttg
      541 cctttctcag ccacttgcgc ccggtgatac tgcggccagt tttgcgtgta cttccgcttt
      601 tcgatcttcg gcgcttcggg aagctctgcg ccgccctcgc gctctttagc atactgagcg
      661 gcgaagatgt gcttgcatat ccggtcgttc ttcttgcagt cgggacaggt acaagtcggc
      721 gggtttatgt cgaggtcaac ggcgtacttt ttggcgctcg tttgcgacgg gactatccag
      781 agattttcaa cgcgggtgat ttccgaggag tgggctatct gtagcccttt ggcttcgcgt
      841 aactgcacag ggtctattca ttctcggagt ctgatgccga ccgccgcgag ggctaacgcc
      901 gggcgggcgg cgtaacggcg gcaggcggcg gcgatattct ccgcgccaca cagccgcaac
      961 gccgagatag cgacgtttct gagcgccgcc atcacctgcg gggcgtgccc ggctctgacc
     1021 tgcgagcggt cttcatcgaa ggtcacgtcg cgcacataat gcagcttgtt ctcgacgtgc
     1081 cagtgttcgc gccacagtcg gaggagttgc gcgggcgtgg cacgctccgg cgcgaggctc
     1141 gtgatgccat agacgatttc atgccgggtc tcggccgtgc gcttgttggt gacctgacgg
     1201 cgcaactcca gaacctgggc gtgaccgggc cactccacat agccttgcag ggccgtggag
     1261 gccgacaggc agcgcacctc gatgcggtcg ccgtgctggt cagtggtctg tgcgtgcccg
     1321 atggtgtcgg ctaactcttt agcgtgcgcg aagagtaacg cgatgtcaga gcgcaggacg
     1381 ggctgattct ccttgactgg cagcaggtag tcgccgccgc gcgccacaac cgtgcgcgcc
     1441 acctcgcgct gcgtgtgcag cgcgtcggcc gtcaccacgc gcccgtcgag gacaagagcg
     1501 tcgagcagcg gctccaagtg gccgagttcg tggctcttat cggccacggc caactgcgcg
     1561 acgaccacgc cgagccggtg cgagagcgcg ctcacgaggt ggctctcggc cgcgccctgc
     1621 ccggcgctgc cgcgcaaagt cttgccgtct atcgccagag cttcgagcgc ggcggccggc
     1681 gcggcgcagg cggcgaggat gtgctcggcc caccgggtta cgcactgttc gaggcgcgcg
     1741 cagtcgagcc ctttgaagat gcggtgcagc gtgggctgcg aaggcccgcg cccccgccgg
     1801 aggccgaggc gcgagagcca acgcgcgccg tagttgtggc cccagtcggc gatggccgcc
     1861 tgcgagcgcg cgccgcacat gacggcgaca caacacaaga gcaggacggg taagaggtcg
     1921 tagcggcgtc cttgagcttg tcggaagtcc ggcacatctg ctaaagcatc cttgagggac
     1981 ggcaggcgca tgggaacatg gcccccttcg tagaagttgg tctgagcaac ctcattctac
     2041 tgaaggatgc cgtccctccc cacgagaatg aatagaccct gaccttagat ggacaacacg
     2101 ggacggcccg aatataattt cgccacagtc gcaagtaagc tactgacagg ctctcccgcc
     2161 cggtttcgag tttccccttt agccatcggt cggtctccga ctttgtgagc gaactgatgc
     2221 ag
//
LOCUS       DASVES010000056        20886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1111594, whole genome shotgun sequence.
ACCESSION   DASVES010000056 DASVES010000000
VERSION     DASVES010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20886
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111594"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1556)
                     /gene="mgtE"
                     /locus_tag="VF240_02095"
     CDS             complement(180..1556)
                     /gene="mgtE"
                     /locus_tag="VF240_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX7174062.1"
                     /translation="MVVNLQPDLERMIRQREFFALRRALLDYPPPDLAALIAELDTED
                     QIIVFRILPRELAADVFEYMELEAQEQLLKAMTQEHVARLLNDMAPDDRTELLEELPA
                     TVTRQMLALLTPEERTVAVSLLGYPEGSIGRLMTPDYVRVRAEWTVEQALDHIRRHGN
                     DSETLSIIYVVDDRGVLIDDVRIREFLLSPRDRPVSELMDHKFVSLKAADDQETAVAA
                     FRREDRNALPVTDSTGVLIGIVTVDDVLDVAEEEATEDIHKLGGVEALEDPYMQTGFA
                     SMVRKRAGWLIVLFIGEMGTATAMGYFEDEIARAVVLALFVPLIISSGGNSGSQAATL
                     VIRALALGEVTLRDWWRVMRREVMSGLTLGGILGLIGFLRIVLWSRFSDFYGPHYVLL
                     GLTVGLTLVAIVLWGTLAGSMLPFALRRLGFDPATSSAPFIATLVDVTGLVIYFSIAA
                     LLLRGTLL"
     gene            1678..3516
                     /locus_tag="VF240_02100"
     CDS             1678..3516
                     /locus_tag="VF240_02100"
                     /inference="COORDINATES: protein motif:HMM:NF023284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3376 domain-containing protein"
                     /protein_id="HEX7174063.1"
                     /translation="MITRETEIPGSERRRVAEVTDDMTLLVARAAGFSEESDEFLAIR
                     HLVRDWRDRHYLPYLKEGARLEGGKKLQAEFLLDFDMPWAMRRVRFCIGKLNDLACLD
                     DQARQIAEVAARAGQPKQWPSEEREKAEFRVALRRMREGLNKALVDLRGARRKLWSRD
                     FNENPFSPLIARLEIGSPDLVGLLRQPTDQACRDEAERILKTRLKNPARPHGMETRDD
                     AVAALTEEVRGKLETAIKKAKGDCRQAMWPREGPDAENVPHWELFLRDVLWYYYKHFD
                     DFDQISYPILYSTGVGEEADVIDVFRVSPEDARALVNEREDKVDIDGEMKNVQKLAGT
                     TLGNFGAFFESKFRVNDITWGRLDGAERVIAALLPANEDLRRKITEQAHRAILVEEEL
                     SKDEAAAKNDALKKLVWRALDVWDDEPRRTILLNEAAQNLPAESRRFREYLALLAKGA
                     EPRDLFRDKFLKNYEKGRQFTPTATVDSAKRANRVLGNMALGYFPATDGGSRKRKLAM
                     WFGQRLLLFAEAAIEPTGSARRRQRARLIACYLLALLILAFVCLPAFMLWRTAGETER
                     AWSFFLIFVLTLPLALIPLALAAGYTYAAYWLRGRLYTRLPQAGAD"
     gene            complement(3683..3994)
                     /locus_tag="VF240_02105"
     CDS             complement(3683..3994)
                     /locus_tag="VF240_02105"
                     /inference="COORDINATES: protein motif:HMM:NF041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HEX7174064.1"
                     /translation="MKRTEAFKVLRSGALGLTLAALVSAAPAQAQNSNNNNSGRDVTT
                     NRADDNRRVARDDDDRDWGWLGLLGLAGLLGLMPKKRVPVVVNETRDRDVRDPDSRDR
                     R"
     gene            complement(4120..5208)
                     /locus_tag="VF240_02110"
     CDS             complement(4120..5208)
                     /locus_tag="VF240_02110"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX7174065.1"
                     /translation="MAKTVVGLFDSPAAARNAVSELVREGFRHEDISLMAKDAGAVQE
                     KDAKVAYVEEGGHEQVEDMAKGAGTGAAIGGAAGLLLGLTALAIPGIGPILAAGPLAA
                     AIAGAGLGGTAGGLLSGLTRLGLGDDDAHTYAEGLKRGGTILSVETDDREAERAVAVL
                     KKFGAAEIDKRAAEWRGQGWTGFDAASAGDGFETYTGGGEAGREESERARVAGDHVAL
                     PVVEERLEVSKREVARGGVRVESRVTETPVEEDVRLRDERVRVERLPADYTFHGAESE
                     AFKEAVIEFTESHEELSVNKKARVVEEVRIGKEFEEHTETVRETLRHSEVEVEPLEPG
                     RARGASAAGEDAGPLPSSGRRPGDREEF"
     gene            complement(5277..6002)
                     /locus_tag="VF240_02115"
     CDS             complement(5277..6002)
                     /locus_tag="VF240_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX7174066.1"
                     /translation="MSQKRAKVVTVTDSEGLRGTIDTASPTLNKRDRQALVYMEDGQQ
                     LLVPIDELQKQEDGSFTLPFSLRQLASDPQSILAGRAGADGDSLADARLMGDGEEATV
                     VVPVIAETLQVEKRTVESGGVRVRKVVHEREEVVDEPLMHEEVQVKRVAVGRIVDGPV
                     PVRHEGDTMIIPLLEEVLVVEKRLMLKEELHVRKESVETHRPQRVTLRSEEAFVERIG
                     DEKAPGDASGDDDETVLAQSPSE"
     gene            complement(6209..7144)
                     /locus_tag="VF240_02120"
     CDS             complement(6209..7144)
                     /locus_tag="VF240_02120"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX7174067.1"
                     /translation="MAKETGAAAAKNSQVVSGMFNGRDDAERAYQSLQERGYSKDEIN
                     VIMSEDTRDKHFPEGSAAADTELGSKAAEGAGTGAAIGGGAGAIIGALVGLGSNVVIP
                     GIGLVAGPIAGALAGAGAGGATGGIVGALVGSGIPEEHAKRYEEGVKQGGIYMGVNPR
                     SAEDAEYFERSWSDSRGAGVYRGETAIPVVEEHLEVGKREVERGGVRVERSVQEVPVE
                     EQVRLKEEHVHVERRAVDRPVGAGDEAFREGSFEVTERAEEVVVAKQARVVEEVVVNK
                     EVGERTETVRDTLRRTDVDVREVGDDTTSDTKNRR"
     gene            7433..7894
                     /locus_tag="VF240_02125"
     CDS             7433..7894
                     /locus_tag="VF240_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEX7174068.1"
                     /translation="MLIVGTEWLVEAAGCDAARLRDPERVRAVLERAVAELDLRVVGD
                     VALHKFPGAGGVTGLMLLTESHLACHTYPEFEVATFNLYCCRTRPRWAWEERLTEMLG
                     AARVSVRVIERRVEVDAGDETAGVSLEECGVRLEETEASLALATEVGGAAR"
     gene            7891..9975
                     /locus_tag="VF240_02130"
     CDS             7891..9975
                     /locus_tag="VF240_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEX7174069.1"
                     /translation="MTSLLANCPACGGQVNFKQGSSVVVVCEYCNSVVARTDRGLEDL
                     GRVAEVAESGSPLTLGLRGVYRGTAFELTGHTQLGHSMGGQWEEWYAAFADGRWGWLA
                     EAQGRFYMSFRAPESVRVPPFEQVAPGSPVREIPTPTPLVVSEKGEARYLSAEGEIPY
                     RLTPGQTYRYADLSGPGGTFATIDYGDEPAGVYVGREVTLTDIGIAATERPREREART
                     VAASQLQCPQCGGPLELRAPDIAERVTCPNCGSLLDVNKGQLQYLKTLKFVGYKPEIA
                     LGSVAEFEGQQLTVIGFMTRSVEFEGTRYFWQEYLLYNPAVGFRWLVQSDGHWSYVVT
                     VPPGEVSEVGNFAHYQGTKFKKFQEAVARVEYVAGEFYWKVTAGERVLAVDYVKAPRM
                     LSKEVSGKFAKGDDTRAMEATEINWSLGTYVPVREVKEKFKLETLPQPSNVAPNQPFP
                     HKSVYKLWPLLLLAMLVVGVLTTMLDARRKIFEQIFSLRPQTAATPPVPAATPNVIRL
                     PGGRTMTVPTMPTPLAMPVAPPASAQGQQVLFTEAFEIAPRQNLRVTAHANVENSYLY
                     VGGDLINEETGLVQQFDIPIEMYSGVEDGESWREGDNEQKEYISALPGGRYVMRLEAQ
                     WEDWKTPTAPLLQVRVEQGVTRGLNFFLALLAISLIPIIVLIRHMSFERRRWAESSFN
                     PYDSGGSGDDDE"
     gene            10035..10397
                     /locus_tag="VF240_02135"
     CDS             10035..10397
                     /locus_tag="VF240_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174070.1"
                     /translation="MAEKVIATLSAQSGLELGYDEPSVEWVDGFIERQRLRFSGEEAG
                     GLVNVIGAFVGECVIANHGGRWREDEHGWGVCFDDGNAAYPFAKVRKQFDNGREGGDS
                     VHSFYETIGVLFKRSDDV"
     gene            10390..10569
                     /locus_tag="VF240_02140"
     CDS             10390..10569
                     /locus_tag="VF240_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174071.1"
                     /translation="MSKFYVIFGALVLMFYSASAWLGWEIGSPRRQQLPPEYRTAGGY
                     RNFNYFWHSGYRGGK"
     gene            10566..10850
                     /locus_tag="VF240_02145"
     CDS             10566..10850
                     /locus_tag="VF240_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX7174072.1"
                     /translation="MMLLEKLTLSALAAHVPTALGVVKMEVVMEVLITTAIFTLFGLL
                     VFGLAYMIILKATPFSIRKEIEEDQNVALAVVIGAVIIGMALIISAAIQG"
     gene            10948..12471
                     /locus_tag="VF240_02150"
     CDS             10948..12471
                     /locus_tag="VF240_02150"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX7174073.1"
                     /translation="MNRTPILFLNVLIIATCGLVYELLAGTLASYVLGDSVTQFSLII
                     GIYLFAMGVGSWLSRFIDKELARRFIDVELAVAILGGASAPLLFLSFANLTYFHVILY
                     GVVFLIGTLVGLEIPLLMRILKDHLDFKELVARVLAFDYIGALVASLLFPLFLVPRLG
                     LVRTSLVFGIFNAAVALWGTWLMRPLIKGSVAGLRGRSIIVIALLLIGLIKADRLTSL
                     AEDQMFTDDIVYARTTPYQRVVVTRGRAGFQLFLNGNLQFSSTDEYRYHEALVHPALA
                     LSAEAGAAPRRVLVLGGGDGLALREILKYQTVEHVTLVDLDPEMTNLSRRFPPLAELN
                     AHAYDDPRVRVVNEDAMIWLEGEGETYDAAIIDFPDPNSFALGKLYTTRFYRLLQQRL
                     APGAAFSIQCTSPLFARVSYWCILRTIEAAGFHVRAYHAAVPSFGVWGFALARSSAPF
                     EPPRAAHASVPLRFLGEDTMAAMFVIPADLGPVPVEINRLDNQALVRYYEAEWRRYE"
     gene            12586..13431
                     /locus_tag="VF240_02155"
     CDS             12586..13431
                     /locus_tag="VF240_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX7174074.1"
                     /translation="MSDRLKRRVYEIVECYTPAAGGRTVVDRFDFFIMALIVANVAAV
                     MLETVDWIATPYARAFRVFEVFSVAVFTAEYALRLWSCTADPRFAGAFRGRLRFALTP
                     MAIIDVLAIMPFFLEFLAFDLRFVRVLRLFRLFRVFKLARYASSMRSLVDVLRAKKEE
                     LLVTLFALVVLLIFSSSVMYYVENEAQPEKFTSIPASMWWGVATLTTVGYGDVFPVTT
                     LGQFFSAVIAILGVGFFALPTGILASGFAEEIQKRRRREAVICPHCGRDIHEPPKSLT
                     DEAAS"
     gene            13434..15086
                     /locus_tag="VF240_02160"
     CDS             13434..15086
                     /locus_tag="VF240_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7174075.1"
                     /translation="MFTRREIIAAFLGLPAALAACRNDEAPRFPDGEIVGASDGIGHR
                     LRDGLRVEVASEAWERVGVVIVGGGVAGLAAAWRFLREGFDDFVVLELEPAVGGTSRS
                     GEAHGVVPYPWGAHYLPAPSKENRALVTLLDEMGVLEGRDSEGNPVVAEQFLCRDPEE
                     RVFYKGRWYEGLYLHAGESAEDETQLAAFNAEVGRWAAWRDARGRRAFTIPVSACSDD
                     AEATALDRVSMADWMRGRGLDSARLRWLVDYSCRDDYGLTAEQTSAWAGLFYFASRLE
                     QPGQETQPLITWPEGNGRLVAHLHGKARGRVRLGLAAADIVPVEEGGRRGVDVVAVGH
                     DGRSAVGFRAERVVFAAPQFLTRYLLRPYREGGAPAHAREFDYGAWMVANLFLEDRPR
                     SGLGFPLAWDNVLYESPSLGYVVATHQRGLDRGPTVFTYYYPLTDDDPRQARTRLLET
                     DWRSWADITLTDLSRAHPEIRALTRRLDVMRWGHAMIRPRPGFVWGGVRAAAQAPFRG
                     VHFAHTDLSGIALFEEAFDHGLRAAEEILRERGTGALKSMRA"
     gene            15083..16276
                     /locus_tag="VF240_02165"
     CDS             15083..16276
                     /locus_tag="VF240_02165"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7174076.1"
                     /translation="MTRARHEDVTRADVIVVGAGAAGLFCAAEAGRRGRRVVVLEHND
                     LPGRKIRISGGGRCNFTNRDSTPDNFISNNPHFCRSALARYTPDDFVRLVERHQIRFH
                     EKKLGQLFCDASAQEITDMLLEECRRARVTVVCNCRVDAVERASLFVVRTGRGTFTAE
                     SLVVATGGLSVTQLGATDFGYRLARQFGLKIRATRPGLVPFTLPAARLKELGALSGVS
                     VDAVAECNGAEFRENILLTHRGLSGPAILQVSSYWMPGDAVTLNLLPEADAFDWLVSN
                     RAREMTLANLLAGHLPRRFAHAWCEMHAPAKPLRQYTPGELKEAAENLRAWRLTPAGT
                     EGYRKAEVTVGGVDTDELSSKTMESRRAPGLYFIGEVVDVTGHLGGHNFQWAWSSGFA
                     AGQHA"
     gene            17236..18378
                     /locus_tag="VF240_02170"
     CDS             17236..18378
                     /locus_tag="VF240_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174077.1"
                     /translation="MAASTVEVLAERPARATAAVGGWWLFSPRIDLMVFLGSALVSLA
                     LLWVGARAGVLEDESPDWAWVPAVLLVDVAHVYATAFRVYFDAEELKRRLWLYALVPV
                     VSLAMGVALYSESETLFWRTLAYLAVFHFVRQQYGWVALYRARRGERGRVGWWIDAAA
                     VYMATLYPLVYWHAHLPRKFWWFLAGDFGALPLIAERIAWPLYLLALGAYATRSLHGW
                     LVRGAGNPGKDVVVVTTAVCWYAGIVAYNSDYAFTVTNVIIHGVPYFVLVYWYARVRR
                     TEASRPYRLLARGPIVFLATLWLLAYAEELLWDRGVWHERAWLFGAEWDASALKMLLV
                     PLLALPQITHYVLDGFIWRRRSNPSLSLIAPSAATRPPANGAIKEC"
     gene            18691..19407
                     /locus_tag="VF240_02175"
     CDS             18691..19407
                     /locus_tag="VF240_02175"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7174078.1"
                     /translation="MQIFHHSTNTLAKVSIFGGLFIAGAALWVLAEVNRSSYNTGQFI
                     ERQQPVQFSHKHHVGDDGISCVYCHTSVESSSSAGLPPTQTCMNCHSQIWSESPYLEV
                     VRASWRENRPIEWTRVHDLPDYVYFNHGIHVAKGVGCASCHGRIDEMPAVYQAASLQM
                     EWCLACHREPERFVRPKEEIYNMAWNIEADTDDGVRADPENRLGENKSARRLEQAREL
                     LKTYKVRSVATITSCSTCHR"
     gene            19426..>20886
                     /locus_tag="VF240_02180"
     CDS             19426..>20886
                     /locus_tag="VF240_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX7174079.1"
                     /translation="MNIKDTLPELYQLRRPARADEARRKLAGTTGREYWQSLEELAAT
                     PEFEELLHREFPQHAAEWDESTDRRTFLKLMGASLALAGISGCSYQPPETAVPYVRQP
                     EGIVPGKPLFFATAMPFAGGATGLLVRSNMGRPTKVEGNPDHPASLGATDVWAQASVL
                     GLYDPDRSQTVVNRGEVRSYTSFLAEAQTQLGALQNGGEGLRFLVESINSPTMAAQLE
                     EILARFPSARVHQWEPAGANSAAVGARLALGDFASTVYRFDRAERVLSLDSDFLSCGP
                     ASLRYARDFASRRRVREGLAGEINRLYAVESTPTNTGAVADHRLAVRPSQVEQIAARI
                     AAGLGAPSPAGANLDAGLSDEQRRFIDAVVADLGAHRGRSIVIAGDEQSARLHALAHV
                     INGALEGVGQTVVYTEPVEMAPADHVESLRELTEAIDADRVRMLVVVGGNPVYDTPVD
                     LRLDARRMLKVPWTAHLGLHDDETSELCHWHIPEAHF"
BASE COUNT         3296 a         7179 c         6756 g         3655 t
ORIGIN      
        1 accggcctcg tcatctactt cagcatcgcc gcgctgctcc tgcgcggtac gctcctctaa
       61 acacgaacgc ttgcacagca cacacagtac cgcgagcgga agcctcgcgg gtgctgttgc
      121 ggaggcagac ccgctcgcta ccgctcgcgg tactgtgcgt cttgcacgcg cgttcgtctt
      181 cagaggagcg tgccgcgcag gagcagcgcg gcgatgctga agtagatgac gaggccggtc
      241 acgtccacga gcgtggcgat gaagggggcg gaggaggtgg ccgggtcgaa gccgagacga
      301 cggagcgcga aggggagcat cgagccggcg agcgtgcccc acaacacgat ggcgacgagg
      361 gtgagtccga ccgtcagccc gaggagcacg taatgcggcc cgtagaaatc ggagaacctt
      421 gaccacagga cgatgcggag gaagccgatg aggccgagga tgccgccgag cgtgaggccc
      481 gacatcacct cgcgccgcat gacgcgccac cagtcgcgca gggtgacttc gccgagggcg
      541 agtgcgcgga tgacgagcgt ggcggcctgc gagccggagt tgccgcccga ggagatgatc
      601 agcggcacga agagggcgag cacgacggca cgcgcgatct catcctcgaa gtagcccatc
      661 gcggtggctg tgcccatctc gccgatgaag aggacgatga gccatccggc gcgcttgcgg
      721 accatgctcg caaaacccgt ctgcatgtaa gggtcttcga gcgcctcgac gccgccgagt
      781 ttatggatgt cttcggtcgc ttcctcttcg gccacgtcga gcacgtcgtc cacggtgacg
      841 atgccgatga ggacgcccgt cgagtcggtg acgggcaggg cgttgcggtc ttcgcggcgg
      901 aaggcggcga cggccgtctc ctgatcgtcg gccgccttga gcgagacgaa cttgtgatcc
      961 atcagctcgg agacgggccg gtcgcgcggg gagagcagga actcgcggat acgcacgtcg
     1021 tcgatgagca cgccgcggtc gtccacgacg taaattatcg agagcgtctc gctgtcgttg
     1081 ccgtggcggc ggatgtgatc gagagcctgc tcgaccgtcc actccgcacg gacgcgcacg
     1141 tagtcgggcg tcatcagccg cccgatgctg ccctcggggt agcccaggag cgagacggcc
     1201 accgtgcgct cctcgggcgt gagcagcgcg agcatctgcc gggtgacggt ggcgggcaac
     1261 tcttccaaca gctcggtgcg gtcgtccggg gccatgtcgt tgaggagacg cgcgacgtgc
     1321 tcctgcgtca tggccttgag gagttgctcc tgcgcttcga gttccatgta ctcgaacacg
     1381 tcggcggcca gctcgcgcgg caggatgcgg aagacgatga tctggtcttc ggtgtcgagt
     1441 tcggcgatga gggcggcgag gtcgggcggc gggtagtcga gcagcgcgcg gcgcagcgcg
     1501 aagaactcgc gctggcggat catcctctcc aaatccggtt gcaggttaac gaccatctgg
     1561 aatactcgcg gactgacgcg cgcggcgagc gacgcgcgca ggccgggccg cgccttccgc
     1621 gcccgaacac gcgaacggcg aggtggaaac ttttacgctg accaaaacga aggggcgatg
     1681 ataacacgtg agacggaaat tcccggaagc gaacgccggc gcgtcgcgga ggtgactgac
     1741 gacatgaccc tcctcgtggc gcgcgccgcc ggcttcagcg aggagtccga cgagttcctg
     1801 gccatccgcc atctggtgcg cgactggcgc gaccgccact accttccata cctcaaggag
     1861 ggcgcgcggc tggagggcgg caagaagttg caggccgagt tcctgctcga cttcgacatg
     1921 ccgtgggcga tgcgtcgcgt gcgcttctgt atcggcaagc tcaacgacct cgcctgtctc
     1981 gacgatcagg cgcggcagat cgccgaagtg gcggcgcgcg cggggcagcc aaagcagtgg
     2041 ccgagtgagg agagggagaa ggctgagttc cgcgtcgcgc tgcggaggat gcgcgagggg
     2101 ctgaacaaag ccctcgttga cctgcgtggg gcgcgccgca agctctggtc acgcgatttc
     2161 aacgagaacc ccttcagccc gctcatcgcc cgcctcgaaa tcggctcccc cgatctcgtc
     2221 ggactgctgc gccagccgac cgaccaggct tgccgcgatg aggccgagcg gattctgaag
     2281 acgcggctca aaaacccggc gcggccgcac gggatggaga cccgtgacga cgccgtcgcc
     2341 gcgttgaccg aggaggtcag ggggaagttg gagaccgcca tcaaaaaggc gaaaggagat
     2401 tgccggcagg cgatgtggcc gcgcgagggg ccggacgccg agaacgtgcc gcactgggag
     2461 ctgttcctgc gcgacgtgct ctggtactac tacaagcact tcgacgactt cgaccaaatc
     2521 tcctatccca tcctctactc gaccggcgtc ggcgaggagg ctgacgtgat cgacgtcttc
     2581 cgcgtcagcc ccgaggacgc gcgcgcgctc gtcaacgagc gtgaggacaa agttgatatc
     2641 gacggggaga tgaagaacgt gcagaagctc gccggcacga cgctcggcaa cttcggcgcc
     2701 ttctttgagt cgaagtttcg cgtcaacgac atcacatggg gccggctcga cggcgccgag
     2761 cgcgtcatcg cggcgctgct gcccgcgaac gaagacctgc gccggaagat aacggagcag
     2821 gcgcaccgcg ccatcctcgt cgaggaggag ctgtcgaagg acgaggcggc cgccaaaaac
     2881 gacgccctga aaaagctggt ctggcgcgcg ctcgacgtct gggacgacga gccacggcgc
     2941 acaattcttc tgaatgaagc ggcgcagaac ctcccggccg agtcgcggcg ttttcgcgag
     3001 tacctcgccc tgctcgccaa aggggccgag ccgcgcgacc tgttcagaga taagttcctg
     3061 aagaactacg agaagggccg gcagttcacg ccgacagcga cggtagattc ggccaagcgc
     3121 gccaaccgcg tgctcggcaa catggccctc ggctacttcc cggccacgga cggcgggagc
     3181 cggaagcgaa agctcgccat gtggttcggc cagcggcttc tcctcttcgc tgaggcggcc
     3241 atcgagccga ccgggtcggc ccgccgccgg cagcgtgcga ggctcatcgc gtgctacctg
     3301 ctggcgctcc tgatactcgc cttcgtctgt ctgcccgcct tcatgctgtg gcgcacggca
     3361 ggcgagacgg agcgcgcgtg gagcttcttc ctgatcttcg tcctcacgct cccgctggcg
     3421 ctcatcccgc tggcgctggc ggccggctac acttacgcgg cttactggct caggggcagg
     3481 ctctacacgc gcttgccgca ggccggggcc gactgaaggc gacgcccgcg ggggaatgtg
     3541 gaggcgatga agacgcccgc gcgcgccggc gtccgtgtct atagtcagcg cgggcactgg
     3601 cctttcgtgc ggaaagtgac gcgggcgccg ggaagttgtt tcccggcgcc cgcgttgtgt
     3661 aaaggatgac ggcggcggcg ctctagcgac gatctcgtga gtcggggtcg cgcacgtcgc
     3721 ggtcgcgtgt ctcgttgacg acgacgggca cgcgcttctt gggcatcagg ccgagcaggc
     3781 cggcgaggcc gagcagaccg agccagcccc agtcgcggtc gtcgtcgtcg cgggcgaccc
     3841 gccggttgtc gtcggcgcgg ttggtcgtca cgtcacgtcc agagttgttg ttgttcgagt
     3901 tctgcgcctg agcgggcgcg gccgacacga gcgccgcgag cgtcaggccg agcgcgcccg
     3961 agcgcagcac cttgaaagct tcagtccttt tcatcgttcc cattctcctg ttcatatcaa
     4021 acaagtttca gatagttcgg tggcgagggg gcaaggctgt ttcatgtgtg cggggctgat
     4081 ccgacatgcg accgccccgc catgagggtt catcgacgct taaaactctt cacggtcgcc
     4141 cggccggcgg cccgacgagg gcagcggccc cgcatcttcg cccgcggccg acgcgcctcg
     4201 cgcgcgcccc ggctccagcg gctcgacctc gacctcgctg tgacgcaacg tctcgcgcac
     4261 cgtctccgtg tgctcctcaa actccttgcc gatacgcacc tcctcgacca cgcgcgcctt
     4321 cttgttcacc gacagctctt cgtgcgattc cgtgaactcg atgaccgcct ccttgaaggc
     4381 ttccgactcc gcgccgtgga aagtgtagtc ggccggcagg cgctcgacgc ggacgcgctc
     4441 gtcgcgcagg cgcacgtcct cctcgacagg cgtctctgtg acgcggctct cgaccctcac
     4501 gccgccgcgc gcgacctcgc gcttgctcac ttccagacgt tcctccacaa ccggcaacgc
     4561 cacatggtcg ccggccacac gcgctctctc agactcttcg cgcccggcct cgccgccacc
     4621 cgtataagtc tcgaagccgt cgccggccga cgcggcgtcg aagcccgtcc acccttggcc
     4681 gcgccactcg gcggcgcgct tgtcgatctc ggctgcgccg aatttcttca acacggcgac
     4741 ggcgcgctcc gcctcgcggt cgtccgtctc gacgctcaaa atcgttccgc cgcgcttcag
     4801 cccttcggcg taggtgtgtg cgtcgtcgtc tccgaggccg agtcgcgtca gcccgctcaa
     4861 gagtccgccg gcagtgccgc cgagtcccgc cccggcgatg gccgcggcga gcggccccgc
     4921 ggcgaggatg gggccgatgc cggggatggc gagcgcggtg agtccgagca gcagcccggc
     4981 cgcgccgccg atggccgcgc ccgtgcctgc gcccttcgcc atgtcctcca cctgctcgtg
     5041 gccgccttcc tcgacgtagg cgaccttcgc gtctttctct tgcactgccc ctgcgtcctt
     5101 cgccatgagg ctgatgtctt cgtggcggaa accttcacgc acaagctcac tcacggcgtt
     5161 gcgcgccgcg gccggactat cgaacaaacc aacaactgtc ttcgccatct cttccccctt
     5221 aacttctcac aaacatgagg acggtcgaac gccaacgccg acgtgcgcga cgcgcgttac
     5281 tccgacggac tttgcgcgag caccgtctcg tcatcgtcgc cgctggcgtc gcccggcgcc
     5341 ttctcgtcgc cgatgcgctc gacgaacgcc tcctcgctgc gcagcgtgac gcgctgcggc
     5401 ctgtgcgtct cgaccgattc tttcctgacg tgcagttcct ctttgagcat caggcgcttc
     5461 tcgacgacca gcacttcttc cagaagcgga atgatcatcg tgtcgccttc gtgccggacg
     5521 gggacggggc cgtcaacgat gcgtcccacc gccacgcgct tgacctgcac ttcctcgtgc
     5581 atcagcggct cgtctacaac ttcctcacgc tcgtgtacta ctttccgcac gcgcacgccg
     5641 cccgactcaa ccgtgcgctt ctccacctgc aacgtctcgg cgatgacggg tacgacgacc
     5701 gtcgcctcct caccatcgcc cataagtctt gcatcggcga ggctgtcacc gtcagcgccc
     5761 gcgcgcccgg cgaggatcga ttgcgggtcg ctcgcgagtt gccgcaggct gaagggcagt
     5821 gtgaaactgc cgtcctcctg cttctgcaac tcgtcgatgg gcacgagcag ttgctggccg
     5881 tcttccatgt agacgagcgc ctgccggtcg cgcttgttga gcgtcggcga cgccgtgtcg
     5941 atagtgccgc gcagtccctc gctgtcggtg acggtaacga cttttgctct cttctgactc
     6001 atcgctctgc cccttcaagt ctcgtcgccg cgccctcgcg cggcgtcgtt aacaaatcca
     6061 tccggcccac gcgcgtaggc tgcgcgtccg aacatttcga taaactgcaa gtgagccatg
     6121 cgcggcgacg ctcgtcttca aactactcgc gagcgccgcc gcgccggccc gaagccgtga
     6181 catgacggcc gttcaagtgc gtcgccgctt agcgacgatt cttcgtgtcg ctcgtcgtgt
     6241 catcgcctac ttcgcgcacg tcaacatcgg tacgtcgaag tgtgtctcgt accgtttcgg
     6301 tgcgctcgcc gacttctttg ttgacgacga cctcttcgac aacgcgcgcc tgcttcgcca
     6361 cgacgacttc ctcggcccgc tccgtcacct cgaaggagcc ttcgcggaaa gcctcgtcgc
     6421 ccgcacccac gggacgatca accgcgcggc gctcgacgtg gacgtgctct tccttcagac
     6481 gcacctgctc ctcgacgggc acttcctgga cgctgcgttc gaccctcacg ccgccgcgct
     6541 cgacctcgcg cttgcccact tccagatgct cctcgaccac gggaatcgcc gtctcgccac
     6601 ggtagacgcc cgcgccgcgg ctgtcgctcc agctccgctc gaagtactcg gcatcttccg
     6661 cgctgcgcgg gttgacgccc atgtagatgc ccccctgctt gacgccctcc tcgtaccgtt
     6721 tggcgtgctc ctcggggatg ccgctgccga cgagcgcgcc gacgatgccg ccggtcgcgc
     6781 cgcccgcgcc cgcgccggcg agagcgcccg caatcggccc ggccacgagg ccgatgccgg
     6841 ggatgacgac gttcgagcct agccccacga gcgcgccgat gatggcgccg gcgccgccgc
     6901 cgatggccgc gcccgtgccc gcgccctcgg ccgccttgct gccgagttcc gtgtcagccg
     6961 ccgcgctacc ctctgggaaa tgtttatcgc gcgtgtcttc cgacatgatg acgttgattt
     7021 cgtccttgct gtatccgcgc tcctgaagcg actgataggc gcgctcggcg tcgtcacgac
     7081 cgttgaacat cccactgacc acctgtgaat tcttcgccgc cgcggcgccc gtttccttag
     7141 ccattagtta tctcctccat gtaaattaaa aaccttcgcc gaagacagac cacggccccg
     7201 acgaggttgg cgaccgtgtt ctgctttgta tgatttcgcg ccgaggcgcg aacgttgagc
     7261 gtgagaagca agcaccgtgc cgccgtgcgc caacgagtgt gagcgcggcg cggtcgcggg
     7321 ctttcatcag gaagggtatt gcaaacaaag cggctttgcc atatcctccc tccgtcaaaa
     7381 tgttcgaggt cttcgttcgg cgccactcga aggttcttct ccaattcaat ctatgctgat
     7441 cgtcggaacc gaatggctgg tggaggcggc gggctgcgac gccgcgcgtt tgcgcgaccc
     7501 ggagcgcgtg cgcgccgttc tggagcgggc cgtcgccgag cttgacctgc gcgtcgtggg
     7561 cgacgtggcg ctgcacaaat ttccgggcgc gggcggcgtg accggcctca tgctgctcac
     7621 cgagtcgcac ctcgcctgcc acacttaccc ggagttcgag gtggcgacct tcaatctcta
     7681 ttgctgtcgc acgcgtccgc gctgggcgtg ggaagagcgg ctcaccgaga tgctcggcgc
     7741 ggcgcgcgtg tcggtgcgcg tcatcgagcg ccgcgtggaa gttgacgcgg gcgacgagac
     7801 ggccggcgtg agtcttgaag agtgcggcgt gcggcttgaa gaaacagaag cgtcgctcgc
     7861 cctcgcgacg gaagtcggag gggccgcgcg atgacatcgc ttctggcgaa ctgccccgcg
     7921 tgcggcgggc aggtcaactt caagcaaggc tcctccgtcg tcgtcgtatg cgagtactgc
     7981 aactcggtcg tcgcgcgcac cgaccgcggg ctggaagatt tggggcgtgt ggcggaggtg
     8041 gccgagtccg gctcgccgct cacgttgggt ctgcgcggcg tctaccgcgg cacggccttc
     8101 gagctgacgg gccacacgca gctcggccac tcgatgggcg gccagtggga agagtggtac
     8161 gcggccttcg ccgacggccg ctggggctgg ttggccgaag cgcaggggcg cttctacatg
     8221 agtttccgcg cgccggaatc ggtgcgcgtc ccgccgttcg agcaggttgc gccggggtcg
     8281 cccgtgcgcg agattcccac gccgacgccg ctcgtcgtct ccgagaaggg cgaggcccgc
     8341 tatctctccg ccgagggcga aatcccctac cggctcaccc ccggacagac ctaccgctac
     8401 gccgacctct ccgggccggg cggcaccttc gccaccatcg actacggcga cgagccggcc
     8461 ggcgtctacg tcggacgcga ggtcacgctc acggacatcg ggattgcggc gactgaaaga
     8521 ccgcgcgagc gcgaggcgcg aaccgtcgcg gcctcgcaac tccagtgccc gcagtgcgga
     8581 ggccccttag agcttcgcgc gcccgacatc gccgagcgcg tcacctgccc gaactgcggc
     8641 tcgctcctcg atgtcaacaa gggacagctc caatacctca agacgctcaa gttcgtcggc
     8701 tacaagccgg agattgcgct cggctcggtc gcggaattcg aggggcagca gttgacggtc
     8761 atcgggttta tgacccgcag cgtcgagttc gagggcacgc gctacttctg gcaggagtac
     8821 ctgctctaca acccggcggt cggcttccgc tggctcgtgc agtcggacgg ccactggagc
     8881 tacgtggtga ctgtgccgcc gggcgaagtt tcggaggtgg gcaacttcgc tcactatcag
     8941 ggcacgaagt tcaagaagtt tcaggaggcc gtcgcgcgcg tcgagtacgt ggccggcgag
     9001 ttctactgga aggtcacggc gggcgagcgc gtcctcgccg tcgattacgt gaaggcgccg
     9061 cggatgctct cgaaggaggt ctccggcaag ttcgcgaagg gcgacgacac gcgcgcgatg
     9121 gaggccaccg agatcaactg gtcgctcggc acatacgtcc ccgtgcgtga ggttaaggaa
     9181 aagttcaagc tcgaaaccct gccacaacct tcgaacgtcg cgcccaacca gccgttcccg
     9241 cacaagagcg tctacaagct ctggccgctg ctgctgctcg ccatgctggt cgtcggcgtg
     9301 ttgacgacga tgctcgacgc gcgccgcaaa atcttcgagc agatcttcag cctccgcccg
     9361 cagacggcgg cgactccgcc cgtgccggcc gcgacgccga acgtcatccg cctgcccggc
     9421 gggcgcacca tgaccgtccc gaccatgccc accccgctcg cgatgccggt cgcgccgccc
     9481 gccagcgcgc aggggcaaca ggtgctcttc accgaagcct tcgagatcgc gccgcgccag
     9541 aacctgcgcg tcacggcgca tgccaacgtc gagaacagct acctctatgt cggcggcgac
     9601 ctcatcaacg aggagacggg gctggtgcag cagttcgaca tccccatcga gatgtactcg
     9661 ggagtggagg acggcgagtc ttggagggag ggcgacaacg agcagaagga gtacatctcg
     9721 gcgctgccgg gcgggcgcta cgtgatgcgc ctcgaagcgc agtgggagga ctggaagacg
     9781 cccaccgcgc cgctgcttca ggtgcgcgtc gagcagggcg tgacgcgggg cctgaacttc
     9841 ttcctcgcgc tcctcgccat ctcgcttatc cccatcatcg tcctcatccg gcacatgagc
     9901 ttcgagcgcc gccgctgggc cgaaagctct ttcaatccat acgactcggg cggctcgggc
     9961 gacgacgacg agtgagtgcg ggcgcgggcg gaaatttcaa ggggggagtg aatttggaag
    10021 agagattcag agagatggcg gagaaggtga tcgcgacgtt gtccgcacag tcggggttgg
    10081 agcttggata tgacgagccg tcggtcgagt gggtggacgg cttcatcgag cgccagcggc
    10141 tgcgcttctc cggcgaggag gctgggggac tcgtcaacgt tatcggcgcc ttcgtcggcg
    10201 agtgcgtgat cgccaaccac ggcgggcgct ggcgcgagga cgagcacggg tggggcgtct
    10261 gcttcgacga cgggaacgcg gcctacccgt tcgcgaaggt gcgtaagcag ttcgacaacg
    10321 gccgggaggg cggcgattcc gtccacagtt tctacgagac gataggcgtg ctcttcaaga
    10381 ggtcagacga tgtctaagtt ttacgtgatt ttcggcgcgc tggtgctcat gttctactcg
    10441 gcctcggcgt ggctggggtg ggaaatcgga agcccgcgcc ggcagcagct cccgcccgag
    10501 taccgcacgg cgggcggcta tcgcaacttc aactacttct ggcattccgg ctaccgcgga
    10561 ggaaaatgat gctgcttgag aaactgaccc tttcggcgct cgccgcgcac gtgcccacgg
    10621 ccttaggcgt cgtgaagatg gaagtcgtga tggaggtgct gataacgacc gccatcttca
    10681 cgctcttcgg cctcctcgtc ttcggcctcg cctacatgat catcctgaag gcgacgccct
    10741 tctcgatccg caaggagatc gaagaagacc agaacgtcgc cctcgccgtc gtcatcggcg
    10801 cggtcatcat cggcatggcg ctcatcatct ccgccgccat ccagggatga gagcagagtg
    10861 agcggcaagc agtgagcagt gagcagtcag gacaggacga gtgagtgttt ttaactgctt
    10921 actgcttact gctcactgct tactcttatg aaccgcaccc ccatcctttt tctcaacgtc
    10981 ctgatcatcg ccacctgcgg cctcgtctac gagctgttgg cgggcacgct cgcctcctac
    11041 gtcctcggcg actcggtcac gcagttctcc ctcatcatcg gcatctacct cttcgccatg
    11101 ggcgtcgggt cgtggctctc gcgcttcatc gataaggagt tggcgcgccg cttcattgac
    11161 gtggagctgg ccgtggccat cctcggcggg gcgtcggccc cgctgctgtt cctctccttc
    11221 gccaacctca catacttcca cgtcatcctc tacggcgtgg tcttcctcat cggcacgctc
    11281 gtcggcctcg aaatccccct gctgatgcgg atcctcaagg accatctcga cttcaaggag
    11341 ctggtcgcgc gcgtgctcgc cttcgactac atcggcgcgc tcgtcgcctc gctgctcttt
    11401 cccctcttcc tcgtcccgcg tctcggcctc gtccgcacgt cgctcgtctt cggcatcttc
    11461 aacgcggccg tcgccctctg gggaacgtgg ctgatgcgcc cgctcatcaa gggcagcgtc
    11521 gcgggtctgc ggggccgctc catcatcgtc atcgcgctcc tgctcatcgg tctcatcaag
    11581 gccgaccgcc tgacctcgct cgccgaggat cagatgttta cggacgacat cgtctacgcg
    11641 cgcaccacgc cctaccagcg cgtcgtcgtc acgcgcggac gcgccggctt ccagctcttc
    11701 ttaaacggca acctccagtt cagctcgacg gacgaatatc gctatcacga ggcgctcgtc
    11761 cacccggcgc tggcgctctc ggcggaagcg ggcgccgcgc cgcgccgcgt gctcgtgctc
    11821 ggcggcggcg acggcctcgc gctccgggag attctgaagt atcagacggt cgagcacgtc
    11881 acgctcgtcg atctcgaccc ggagatgacc aacctctcgc gccgcttccc gccgctcgcc
    11941 gaactcaacg cgcacgccta cgacgacccg cgcgtgcgcg tcgtcaacga ggacgcgatg
    12001 atctggctcg aaggcgaggg cgagacttac gacgccgcca tcatcgactt ccccgacccc
    12061 aactcgttcg ccctcggcaa gctctacacg acgcgcttct accggctgct gcaacagagg
    12121 ctcgcgcccg gcgcggcctt ctccatccag tgtacctcgc cgctcttcgc gcgcgtctcc
    12181 tactggtgca ttctgcgcac catcgaggcc gccggcttcc acgtccgcgc ctaccacgcg
    12241 gccgtgccct ccttcggcgt ctggggcttc gcgctggcgc gctcgtccgc gcccttcgag
    12301 ccgccgcgcg cggcgcacgc gtccgtcccc ctgcgcttcc tcggcgagga tacgatggcg
    12361 gcgatgttcg tcatcccggc agacctcggc cccgtccccg tcgagatcaa ccgactggac
    12421 aatcaggcgc tcgtccgcta ctacgaggcc gagtggcgga ggtatgaata ggagtgagca
    12481 gtgagcagtg agcagtaagc agtaaaaaca agttcggcgg tgagcggcga gcttcgagag
    12541 cggcctactg ctcactgctt actgctcact gctcactttc taaccatgtc cgacagactc
    12601 aagagacgcg tctacgagat cgtcgagtgc tacacgccgg cggcgggcgg gcgcacggtc
    12661 gtggatcggt tcgacttctt tatcatggcg ctcatcgtcg caaacgtcgc cgccgtgatg
    12721 ttggagacgg tggactggat cgccacgccg tatgcgcgcg ccttccgcgt cttcgaggtc
    12781 ttctcggtcg cggtcttcac ggccgagtac gcgctccgcc tctggtcgtg tacggccgac
    12841 ccgcgtttcg cgggagcctt ccgcggccgg ctgcggtttg cgctcacccc gatggcgatc
    12901 atcgacgtgc tcgccatcat gcccttcttc ctcgaattcc tcgccttcga cctccgcttc
    12961 gttcgcgtcc tgcgcctctt ccgcctcttc cgcgtcttca agctcgcgcg ctacgcctcc
    13021 tccatgcgct cgctcgtcga tgtgctgcgg gcgaagaagg aggagctgct ggtgacgctc
    13081 tttgcgctcg tcgtcctgct catcttctcg tcgagcgtga tgtactacgt ggagaacgag
    13141 gcgcagccgg agaagttcac gagcatcccg gcttcgatgt ggtggggcgt ggcgacgctg
    13201 acgaccgtcg gctacggcga cgtctttccc gtcacgacac tggggcagtt cttcagcgcg
    13261 gtaatcgcca tcctcggcgt cggcttcttc gcgctcccca cgggcatcct cgcctccggc
    13321 ttcgccgagg agattcagaa gcgccgacgc cgcgaggccg tcatctgccc gcactgcggg
    13381 cgcgacatcc acgagccgcc aaagtctctg accgatgagg cggcaagtta aggatgttca
    13441 cacgccgcga aataatcgcc gccttcctcg gtctgccggc cgcgctcgcc gcctgccgta
    13501 atgacgaagc gccgcgtttc cccgatggcg agatcgttgg cgcatccgac ggcatcggcc
    13561 atcgcctgag agacggcctg cgcgtcgagg tcgcaagcga agcgtgggag cgcgtggggg
    13621 tcgtcatcgt cggcggcggc gtggcggggc tggcggcggc gtggcgtttc ctgcgcgaag
    13681 ggttcgacga cttcgtcgtg ctcgagctgg agccggccgt gggcggcacc tcgcgcagcg
    13741 gcgaggcgca cggcgtcgtc ccctacccgt ggggcgcgca ctacctgccc gccccgtcga
    13801 aggagaaccg cgcgctcgtc acgctcctcg acgagatggg cgtgcttgaa gggcgcgaca
    13861 gtgaagggaa ccccgtggtc gccgagcagt tcctctgccg cgaccccgag gagcgggtct
    13921 tctacaaggg acgctggtac gaggggctgt acctgcacgc gggcgagtcg gcggaggacg
    13981 agacgcaact cgccgccttc aacgccgagg tcggccgttg ggccgcgtgg cgcgacgcgc
    14041 gcggacgccg cgccttcacc atccccgtct ccgcctgctc cgacgacgcc gaggccaccg
    14101 cgctcgaccg cgtctcgatg gccgactgga tgcgcgggcg cggactcgac agcgcacgcc
    14161 tgcgctggct cgtcgattac tcctgccgcg acgattacgg cctgaccgcc gagcagacga
    14221 gcgcgtgggc cggactcttc tacttcgcct ctcgcttgga acaacccggt caggagacgc
    14281 agccgctcat cacctggccc gagggcaacg gccgcctcgt cgcgcatttg cacgggaagg
    14341 ctcgtgggcg cgtgcgtctc ggtctcgcgg cggcggacat tgtgccggtc gaagagggag
    14401 gccgccgggg cgtcgatgtc gtcgccgtcg gtcacgacgg gcgcagcgcc gtcggcttcc
    14461 gcgctgagcg cgtcgtcttc gccgcgccgc agttcctcac gcgctacctc ctgcgcccct
    14521 accgcgaggg cggcgcgccc gcgcacgcgc gcgagttcga ctacggcgcg tggatggtgg
    14581 cgaacctctt cctcgaagac cgcccgcgct ccggcctcgg cttcccgctc gcgtgggaca
    14641 acgtgctcta cgaaagcccc tcgctcggct acgtcgtcgc cacgcaccag cgcggcctcg
    14701 accgcggccc caccgtcttt acttactact acccgctcac cgacgacgac ccccggcagg
    14761 cgcgcacgcg tcttctggaa accgactggc gttcatgggc cgacatcacc ctcaccgacc
    14821 tctcccgcgc gcatcccgag atacgcgcgc tcacccgtcg cctcgacgtg atgcgctggg
    14881 gccacgccat gattcgcccg cgccccggct tcgtctgggg cggcgtgcgc gccgccgcac
    14941 aagcgccctt tcgcggcgtc cacttcgccc acacagacct ctccggcatc gccctcttcg
    15001 aggaggcttt cgaccacgga ctgcgcgccg ccgaagaaat cttacgcgag cgcgggacgg
    15061 gcgcgctgaa gagtatgagg gcatgacgcg cgcgaggcat gaagacgtga cgcgtgcgga
    15121 cgtgatcgtc gtcggggccg gggcggccgg tctgttctgc gccgcggagg cggggagacg
    15181 cggccggcgc gtcgtcgtcc tcgaacacaa cgacctgccg ggccgcaaga tcagaatctc
    15241 cgggggcggc cgctgcaatt tcacgaaccg cgactcgacg cccgacaact tcatctccaa
    15301 caatccgcac ttctgccgct cggccctcgc ccgttacacg cccgacgact tcgtccgcct
    15361 cgtcgagcgc caccagatac gcttccacga gaagaaactc ggccaactct tctgcgacgc
    15421 gagcgcgcag gagatcactg acatgctcct cgaagagtgc cggcgcgcgc gcgtcacggt
    15481 cgtctgcaat tgccgtgtgg acgctgtaga gcgcgcttcc ttattcgtcg tcaggactgg
    15541 gcgggggacg ttcaccgccg agtcgctcgt cgtggcgacg ggcggcctct ccgtcacgca
    15601 gctcggggcg accgacttcg gctaccggct cgcccgacag ttcggtctga agatacgcgc
    15661 gacgcggccc ggtctcgtgc cgttcacgtt gccggccgcg cgtctgaagg agttgggcgc
    15721 gctgagcggc gtctcggtgg acgccgtggc cgagtgcaac ggcgcggagt tccgcgaaaa
    15781 cattctgctc acgcatcgcg gcttgagcgg cccggccatc cttcaggtct cttcctactg
    15841 gatgccgggc gacgcggtga cgctcaacct tctccccgag gcggacgcct tcgattggct
    15901 cgtgtcgaac cgcgctcggg agatgacgct cgcgaacctg ctcgcagggc atctgccgcg
    15961 gcgtttcgcg cacgcgtggt gcgagatgca cgcgccggca aagcctctgc ggcagtacac
    16021 gccgggcgag ttgaaggagg cggcggagaa cctgcgtgcg tggaggctca cgcccgcggg
    16081 gactgaaggc tacaggaagg cggaggtgac ggtgggcggc gtggacaccg acgagctatc
    16141 gtcgaagacg atggagtcgc gtcgggcgcc ggggctgtac ttcatcggcg aggtcgtgga
    16201 cgtgacgggc cacctcggcg ggcacaactt ccagtgggcc tggtcgtcgg gcttcgccgc
    16261 cggccagcac gcgtgagggc aggcgcgcca cgactacagt accgcgagcg gtagcgagcg
    16321 ggtgtcgcgt gagccaggcg agtcgcccat cacagtcgca cccgcgaggc ttccgctcgc
    16381 ggtactgttt tgagccagcc gtagtacagc ctgcgtcccc aaaaggaaat tgcgccgcga
    16441 cgaccgaagc catcgcggcg caatcttttc atcccgagag ccgccgaagc gtctgcaagg
    16501 gatgtggcga cttcgctgta cacgcggcag ccgccaaatt tatatcccgg acaccggcgt
    16561 ctttaaaaac ggttctgacg cccgccggtc ggcgatgtag gatgcgcttt cgcgaacccc
    16621 cgcaattacc tgtgaaggca cttgcgtcgc cccttggttt ttgttgtggt ggctttcctc
    16681 caccgccggt ttacgttccg gcgaaacgag gcacgtgtcc gaagacgcgt gcgactgcgc
    16741 tgctcgaagg ccgatcaatt cctccgagtg ccacccgttt ttatccgggc gctcgggcgg
    16801 gtctgccgac ccgaccctag ccggcctttc ggccgcgacg cggtcgtcag agcaaatgca
    16861 aaccgctctg cctctgccct ctgaacggcg cgttgccgca tccgtccacc tctctccgcg
    16921 ccggtcgctg tccccttgag aaccgcgacg ggacgtgcaa gatacgctcg cctcactgac
    16981 ctgtcaagag ctattttcgc atttctcatc caaagcgttc gcctgtggat aaaggtgtgc
    17041 ctgtggaaat tcggagacgt ttttcgggcg taaaaatgag cggccccgag ccgcctgtgg
    17101 aaatcacgtt gtggtgaaac ctgaagccgc gcgaagtcac accagcatcg tttcaaattt
    17161 ctcgcctcgc gacgccgcgc aggtataatg ccgcgtcgct tttgtactcc gcgagtcacc
    17221 gaacttaaaa ccgacatggc cgccagcact gtagaagtct tagccgagcg ccccgcaaga
    17281 gccacggccg ccgtgggcgg atggtggctc ttttcgccgc gcatcgatct gatggtcttt
    17341 ctggggagcg cgctcgtgtc gctcgcgctg ctctgggtgg gcgcgcgcgc cggggtgctc
    17401 gaagacgagt cgcctgactg ggcatgggtg ccggccgtgc tgttggtgga cgtggcgcac
    17461 gtctacgcga cggcgtttcg agtctacttc gacgcagagg aattgaagcg ccggctctgg
    17521 ctctacgcgc tcgtccccgt cgtctcgctc gccatgggcg tggcgctcta ctcggagagc
    17581 gagaccctct tctggcggac gctcgcctac ctcgccgtct tccacttcgt gcgacagcaa
    17641 tacgggtggg tggcgctcta tcgcgcgcgc cggggcgagc gcgggcgcgt gggctggtgg
    17701 atcgacgccg cggccgtcta catggcgacc ctctacccgc tcgtctactg gcacgcgcac
    17761 ctgccgcgaa agttctggtg gtttctggcg ggcgacttcg gcgcgctccc cctcatcgcc
    17821 gagcgcatcg cgtggcccct ctacctgctc gcgctcggcg cctacgcgac gcgctccctc
    17881 cacggctggc tcgtgcgcgg cgcaggcaat cccggcaagg atgtcgtcgt cgtgacgacg
    17941 gccgtctgct ggtacgccgg catcgtggcc tacaactccg actacgcctt cacggtgacg
    18001 aacgtcatca tccacggcgt gccgtacttc gtgctcgtct actggtacgc gcgcgtgcgc
    18061 cgcacggaag cgagccggcc ctaccgtctg ctcgcccgcg gccccatcgt ctttctggcg
    18121 acgctgtggc tgctcgctta cgccgaagaa ctgctgtggg acaggggcgt gtggcacgag
    18181 cgcgcgtggc tgttcggcgc ggagtgggac gcctcggcgc taaagatgtt gctggtgccg
    18241 ctcctcgccc tgccgcagat cacgcactac gtgcttgacg gctttatctg gcgacgccga
    18301 agcaacccga gcctttcgtt gatcgcgccg tcggcggcga cgcgcccgcc cgccaacggc
    18361 gcaattaagg agtgctaatg aagcgggcga ggtttattca tagcgggggt caactatgtg
    18421 cttgcccgcc gcgaaaaaaa gtagtatctc ttaggtcatt caaagcagta cctgatgtta
    18481 gcagggcgga atacggcggt aagaacacct gtcgtctgaa agggacgggg taactttacc
    18541 cggcggacgc ttgtacccac gcgctgcgtg tgagagtggc gcgtcgctgc gccgccgcca
    18601 cggtatcgcc acaaacgcac gacacaaagg caacacggtt gccgcaagct acagactgtc
    18661 cgtagagact tccgaggcgc gctccgggca atgcagattt tccaccacag cacgaacacg
    18721 ctcgccaagg tgagcatctt cggcggtctc ttcatcgccg gggccgccct gtgggtgctg
    18781 gccgaggtca accgctcctc ctacaacacg gggcagttca tcgagcgcca gcagcccgtc
    18841 cagttctcgc acaagcacca cgtcggcgac gacggcatct cttgcgtcta ctgccacacg
    18901 tcggtcgagt cgtcctcctc ggcggggctg ccgccgacgc agacctgcat gaactgtcac
    18961 tcgcaaatct ggtcggagag tccgtacctt gaagtggtgc gcgcaagctg gcgcgagaac
    19021 cggccgatag agtggacgcg cgtccacgac ctgcccgact acgtctactt caaccacggc
    19081 atacacgtcg ccaagggcgt ggggtgcgcc tcctgtcacg gacgcatcga cgagatgccg
    19141 gccgtctatc aggccgcctc gcttcagatg gagtggtgtc tggcgtgcca ccgcgagccg
    19201 gagcgcttcg tccgcccgaa ggaagagatt tacaacatgg cgtggaacat tgaggccgac
    19261 accgacgacg gcgtgagggc cgaccccgag aaccgtctcg gcgagaacaa gtcggcgcgg
    19321 cgtctggagc aggcgcgcga gctgctcaag acctacaagg tgcgctccgt cgccacgatt
    19381 accagttgct cgacgtgtca ccgctgaggc tgtagggccg gttagatgaa catcaaagac
    19441 actctccccg aactctatca actgcgccgc ccggcgcgcg ccgacgaagc gcgccgcaag
    19501 ctcgcgggca cgacggggcg cgagtactgg cagagcctcg aagagctggc ggccacgccc
    19561 gagttcgagg agcttctgca cagggagttc ccgcagcacg ccgccgagtg ggacgagtcc
    19621 acggatcgcc gcaccttcct gaaactgatg ggcgcgtcgc tcgccctcgc cggcatttcg
    19681 ggttgctcct accagccgcc ggagacggcc gtcccttacg tccgccagcc cgaagggatc
    19741 gtgcccggca agccgctctt cttcgccacg gcgatgcctt tcgcgggcgg ggccacgggc
    19801 ctgctcgtca gaagcaacat ggggcggccg accaaagtcg aagggaatcc cgaccacccg
    19861 gcgagcctcg gcgcgacgga cgtgtgggcg caagcctcgg tgctcggcct ctatgacccc
    19921 gaccgctcgc agacggtcgt caatcgcggc gaggtgcgct cctacacgtc cttcctcgcc
    19981 gaagcgcaga cacagctcgg cgcgttacag aacggcggcg agggcttgcg cttcctcgtc
    20041 gagtcgatca actcgccgac gatggccgcg cagctcgaag agattctcgc gcggttcccg
    20101 tcggcgcgcg tgcatcagtg ggagccggcc ggggccaact ccgcggccgt cggcgcgcgt
    20161 ctcgccctcg gcgacttcgc ctcgaccgtc taccgcttcg accgcgctga gcgcgtgctc
    20221 tcactcgact ccgacttcct ctcctgcggc cccgcgagct tgcgctacgc gcgcgacttc
    20281 gcctcgcgcc gccgcgtgcg cgaaggactc gcgggcgaga tcaatcgtct ctacgccgtc
    20341 gagtccacgc cgacgaacac cggcgcggtc gccgaccacc gcctcgccgt tcggcccagt
    20401 caggtcgaac agatcgccgc gcgcatcgcc gccggtctgg gcgccccgtc gccggccggc
    20461 gccaacctcg acgccggact ctccgacgag cagcgccgct tcatcgacgc cgtcgtcgcc
    20521 gacctcggcg cacaccgcgg ccgctccatc gtcatcgcgg gcgacgagca gagcgcgcgc
    20581 cttcatgcct tagcgcacgt catcaacggc gcgctcgaag gcgtggggca gaccgtggtc
    20641 tacaccgagc cggtggaaat ggcgccggcc gaccacgtcg aatctttgcg cgagctgacg
    20701 gaggcgattg acgccgaccg cgtgcggatg ctcgtcgtcg tcggcggcaa tccggtctac
    20761 gacacgccgg tcgatctccg gctcgacgcg cggcggatgc tcaaggtgcc gtggacggcg
    20821 cacctcggtc tccacgacga cgagacctcc gagctatgcc actggcacat ccccgaagcg
    20881 cacttc
//
LOCUS       DASVES010000057         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_529795, whole genome shotgun sequence.
ACCESSION   DASVES010000057 DASVES010000000
VERSION     DASVES010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529795"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VF240_02185"
     CDS             complement(<1..1086)
                     /locus_tag="VF240_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174080.1"
                     /translation="MRSLSPLLTCAAGLAFLFLVGAPSVAQAQSCSSPFFVEQRFPVS
                     GTEETRWRLCWEMRNRNGLIIRDAWFRKAPNAPWVKVIYDARVAEIFVPYHTGSPRFL
                     DMSGFNFGSATLTDKHCPRGVGGTLLSRDVCKEVRDRGLAWNDDSEVRRGQELVLWGV
                     IDAANYNYVIEWTFRDDGVILGRVGATATNLPGIPLEAHMHGPIWRLDIDLNGAGGDS
                     AHLSTHTESFLGLTATDTDPIIAREGGRVWSAPRYNAIHIHDSNLRNANNHHSGYMLI
                     PLRNGTPRHQENFTKNDFWVSRYRSTELAGASVPSYVAPAEPTVNTDNVIWYYGGAHH
                     IPRDEDGEHVNGRFEPLVAHVMWTGFML"
     gene            complement(1083..1829)
                     /locus_tag="VF240_02190"
     CDS             complement(1083..1829)
                     /locus_tag="VF240_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174081.1"
                     /translation="MNFQSRSLRPRRRWRPLPLLLLAALASVATFSLPTSHAGNAPAQ
                     TQGRSPLDPLTPQERERAASIAEGDTRTLALRGTGRQHRVSVELADVKPPEPTPTLTD
                     PDRAPRPGRYAEVLYYRYATNDGLRVIVNLDSGRVQDAARVDSREVPFAFEELEEASA
                     LALQNPQLRGLLGPSADRYRVLSSAPGEDRDALRVEGLRILASSPRDPCWQHRCVSLL
                     FRQGRRYLTYTDVTVDLTTRRVRVERKNPR"
     gene            complement(2251..2772)
                     /locus_tag="VF240_02195"
     CDS             complement(2251..2772)
                     /locus_tag="VF240_02195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX7174082.1"
                     /translation="MSIIGDIIESTKAIIKGMSTTFAYIPREKWTVEYPDEPVTMQPR
                     YRGQHLLHVDELGREKCVACFLCAAACPSDCIYIVAADDPRPPEERTGVDERYASVYN
                     IDYGRCIFCGFCVEACPKDAITHGYNFELSVYNRADLLKTKDDLLITKQKQSANYRVA
                     FADEDVDSEYKVV"
     gene            complement(2907..3335)
                     /gene="ndk"
                     /locus_tag="VF240_02200"
     CDS             complement(2907..3335)
                     /gene="ndk"
                     /locus_tag="VF240_02200"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7174083.1"
                     /translation="MAEQTGNLTFGIIKPDAVRAGKTGTVLQRITDGGFRVRAMKLIH
                     MTRAEAEGFYAVHRERPFFGELTEFMSGGPCVVLALERDGAVKAWRDLMGATDPAKAD
                     DATIRKEFGASIGNNAVHGSDSEENAQVELAYFFSRLEFV"
BASE COUNT          599 a         1025 c         1176 g          680 t
ORIGIN      
        1 tagcataaac cccgtccaca tcacgtgggc cacgaggggc tcgaaccgtc cgttgacgtg
       61 ttcgccgtcc tcgtcgcgcg ggatgtgatg tgcgccgccg tagtaccaga tgacgttgtc
      121 tgtgttgacg gtgggctcgg ccggggcgac gtaagacggg acggacgcgc cggccaactc
      181 ggtcgagcgg tagcgcgaga cccagaagtc gttcttcgtg aagttctcct ggtgtcgcgg
      241 cgtgccgttg cgcaagggga tgagcatgta acccgagtgg tggttgttgg cgttcctgag
      301 gttcgagtcg tggatgtgga tggcgttgta gcggggcgcg ctccaaaccc tcccgccctc
      361 tctcgcgatg atgggatcgg tgtcagtggc cgtgagtccg agaaaagact cggtgtgcgt
      421 gctcaggtgg gccgagtcgc cgcccgcgcc gttgaggtca atgtcgagcc gccagatggg
      481 gccgtgcatg tgcgcttcga gcgggatgcc ggggaggttc gtggccgtcg ccccgacccg
      541 ccccagaatc acgccgtcgt cgcggaacgt ccactcgatg acgtagttgt agttggctgc
      601 gtcgatgaca ccccacaaaa ccagttcctg accgcgacgc acctcggaat cgtcgttcca
      661 ggcgagccct cggtcgcgca cctccttgca cacgtcgcgg ctgagcagcg tcccgccgac
      721 gccgcgcggg caatgcttgt cggtgagtgt ggccgagccg aagttgaaac cgctcatgtc
      781 gaggaagcgg ggcgagccgg tgtggtaggg cacgaaaatc tccgccacgc gggcgtcgta
      841 gatgaccttg acccacggcg cgttgggcgc tttgcggaac cacgcgtcac ggataatcag
      901 cccgttccga ttccgcatct cccagcagag acgccagcgc gtctcctcgg ttccgctgac
      961 gggaaatctt tgttcgacga aaaaggggga cgagcagctt tgggcttggg cgacggacgg
     1021 cgcgccgacg aggaagagga aggcgaggcc cgcggcgcag gtgagcaggg gagagagact
     1081 tctcatctcg ggttcttcct ttctacgcga actcgtcgcg tggtgaggtc aacggtcacg
     1141 tccgtgtaag tcaggtagcg ccgcccctgc cggaagagga gcgagacgca gcggtgctgc
     1201 cagcacgggt cgcggggcga gctggcgagg attctgaggc cctcgacgcg caacgcgtcg
     1261 cgatcttcgc ccggcgcgga gctcagcacg cggtagcggt cggccgacgg gccgagcagg
     1321 ccccgcaact gcgggttttg cagcgcgagg gcggacgcct cctcaagctc ctcgaaagca
     1381 aacggcactt cgcggctgtc aacgcgcgcc gcgtcctgca cgcgtccgct gtcgaggttg
     1441 acgatgacgc gcaggccgtc gttggtcgcg taacggtaat agaggacttc ggcgtaacgg
     1501 ccggggcgcg gcgctctgtc ggggtctgtc agggtgggcg tcggctcggg cggcttcacg
     1561 tcggccaact cgaccgacac gcggtgctgc cggcccgtgc cgcggagagc cagcgtgcgc
     1621 gtgtcgccct cggcgatgct ggcggcgcgt tctcgctcct ggggcgtgag cgggtcgagc
     1681 ggactgcgcc cctgcgtctg ggcgggcgcg ttgcccgcgt gcgaagtggg aagggaaaac
     1741 gttgcgactg aggccagcgc ggccagcaag aggagaggca agggtcgcca gcgccggcgc
     1801 ggacgaagtg aacggctctg aaagttcatg aggaacctcc tttcaggttg tcagagaact
     1861 agccgttggt agcgggagtc tatgtcaatg ggggtaagct ttcaatacaa tttgttgtta
     1921 atgggtcaat ttcaaatccg ccgcgactac tccgcaaccg gattcacccg aggcgatcac
     1981 tctaaatact ctcgcctaca atctgtatgc gatttcacgg taagacattt catgaggccg
     2041 cgcgcgcgcg tggctgaaaa atatagtttt caggtgcaaa ccgacccacc caacaccatt
     2101 ttttggatgc ggcgggcggc cgcgcgggtt cgccccgccc tgtaaccgcg gaagacaaaa
     2161 ggcggaagtg gaaacggccc gtgcgccgtc ccttactccc gcctttttaa ctttggccct
     2221 cggccgcccg atgtccgtca ggcgcgagtt tcagactact ttgtactccg agtccacatc
     2281 ttcgtcggcg aaggcgacgc ggtagttggc cgactgcttc tgtttggtga tgaggaggtc
     2341 gtctttggtc ttgaggaggt cggcgcggtt gtagacggaa agctcgaagt tgtagccgtg
     2401 ggtgatggcg tccttcgggc aggcttcgac acagaagccg cagaagatgc agcggccgta
     2461 gtcgatgttg tagacgctgg cgtaacgctc atcgacgccc gtgcgctcct cgggcgggcg
     2521 cgggtcgtca gccgcgacga tgtaaatgca gtccgacggg caggccgccg cgcacaggaa
     2581 gcaggcgacg cacttctcgc ggccgagttc gtctacgtgc aaaaggtgct ggccgcggta
     2641 gcgcggctgc atcgtgacgg gttcgtccgg gtactcgacc gtccacttct cccgcgggat
     2701 gtaggcgaag gtcgtgctca tccccttaat gatcgccttc gtcgattcga tgatgtcgcc
     2761 gatgatggac atgggaaacc ccgtaaatcg tcaatcgtaa atcgtgaatc gcggttgaag
     2821 gactactcgt cttgagtgat gtatcgcaag tcggattgtc attcaacaat accgatttac
     2881 gattgacgat tcacgattta cggcttttac acaaactcca gccggctgaa gaagtaggcc
     2941 agctcgacct gcgcgttttc ttcggagtcg gagccgtgga cggcgttgtt gccgatggag
     3001 gcgccgaact ctttgcggat ggtggcgtcg tcggccttcg cggggtcggt cgcgcccatc
     3061 aggtcgcgcc acgctttaac cgcgccgtcg cgttcgagcg cgagcacgac gcaggggccg
     3121 cccgacatga actccgtcag ctcgccgaag aagggccgct cgcggtggac ggcgtagaag
     3181 ccctcggcct cggcgcgggt catgtggatg agcttcatgg cgcggacgcg gaagccgccg
     3241 tccgtgatgc gctgcaagac cgtgcccgtt ttcccggcgc gcaccgcgtc gggcttgatg
     3301 attccgaaag taagattgcc tgtctgctct gccatcgttc tcctcaactt aactgtttca
     3361 actttgcggc tgctcacccg ctccctgcac gaagtcgatc aacgcgtcga gcctttgatc
     3421 tgagtgagct tgtgcggcgg cgagttctga cattttcatg ctaagacctt tgagagcctc
//
LOCUS       DASVES010000058         8967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_914907, whole genome shotgun sequence.
ACCESSION   DASVES010000058 DASVES010000000
VERSION     DASVES010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914907"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..819)
                     /locus_tag="VF240_02205"
     CDS             complement(259..819)
                     /locus_tag="VF240_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174084.1"
                     /translation="MLTPHPLNAIHDGWPVLASFSVLLVLFLLIGNRTKGRLLTPEAP
                     RRAVSLELAGTEERAEQIINSWAREGMIGEAYRHLRWDAFLIPVYSTLWALGCVIAAR
                     AFFGRGTTAYDVALAVAWLPWLAGVLDYVENGAMYGMLDGFDGETLPRRAWWCAAVKF
                     AIIIPLGVYSLAGVACYAWKWFRGVL"
     gene            1042..4071
                     /locus_tag="VF240_02210"
     CDS             1042..4071
                     /locus_tag="VF240_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174085.1"
                     /translation="MRSESSSGAGEEVDALVRELPDPEGARLFYERVVAEHPPARRLF
                     ERDAGLLSDALALAAWSPLLGTTLAQQPEHLQWLARERVASRVKTTEELGESLARFAL
                     THTQAGTQVILARFRRRELLRVYLHDLRHTTTLVETTEELSNLADAVLAYALQLAQSE
                     LENLYGAPQCTDERGRKRSASLCIVALGKLGSRELNYSSDIDLLFIYSEDGETSGTGE
                     RGAITNREFFCKLAEGVARLVGQPSGEGAAYRVDLRLRPHGRDGALAVSLREALRYYH
                     ETAHAWELQALIRQRASAGSPQLFARFSEGLRERVYRRDETVAHALFNVRLAKQKIDR
                     FHRREAGGYNVKLGRGGIRELEFIAQALQLAHGGRDPWLHAPHTLISLSRLADRHLVT
                     ERETTSLSDAYTFLRMLEHRLQMEHGLQTHTLPDDPARRSLVARRMHFHGASALEEFE
                     QALRTHIANVRAVYDRVFGDTEDDAAQATATTDAATATPRVTATPAPDARAPAAPEAS
                     PAPRGSALAHPAPDDRGARTPEDDAEKAAHAAAVVFAPHIAGALEETTGKVARRLLDA
                     ARGSLNPKRALALLARVASSLDKSDADVELNLNEEDLGALARLCGGSEFLGEMVAANP
                     ELIPALAARAEAVGERDHRAVLRAAVDGGRSFREELSALRRAWARLLVEIGARDVEGD
                     ITPAESNRLQTTLAAASLNAALLVARREIARHHGPFAAGPRLSVLGLGRLASGGMDYG
                     SDLDIILVYDPKVPPPIGGLSHDESYARLSEPFASALSNLTRDGYLYRVDLRLRPDGR
                     NGPVAVGARSFVNYIRERAGVWELLAYVKLRAVAGDLELGRAVETEARRTVHEAARRA
                     GAEVLRAETRRVRERLEREHTRGQGRHGTDIKYGPGGMLDVYFAARYLQLRDDVADVG
                     DDRSTRATLARLHENGSLGETDFHALDRGYALLRRIDHHLRLLAGRSTRLPAAPDHPV
                     LSDLSRRTGHAAPAALVSDLDAHTAAVRAAYLRVVEG"
     gene            complement(4340..4912)
                     /locus_tag="VF240_02215"
     CDS             complement(4340..4912)
                     /locus_tag="VF240_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174086.1"
                     /translation="MESVYVCSNCRHESASHLAACPACGRGPFYATSFPGRAGGSLPA
                     HAAGHAPSAERACYQCDYAVADASVKKCPQCGKGRVLTRGQVRVLGWVLLVIGAFLVL
                     FMGGITVLVAGIIANANLPGSTNRFAGDEKQMAIMFGLFGLVIAFGFASIVAGASQIR
                     HGRRNKTIVKVVLGIFIAILVVGELVFIFF"
     gene            complement(4974..6305)
                     /locus_tag="VF240_02220"
     CDS             complement(4974..6305)
                     /locus_tag="VF240_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7174087.1"
                     /translation="MKLVCVCALLLALFAANLPSLAQGGARGEAKTVVIKAARMFDGK
                     SDRVVSPGVVVVSGGKIQAAGPGVAIPAGAEVIDLGDATLLPGFMDAHTHLSGESSDD
                     WKQDELDELKKSAAERALDATVHARKTLLAGFTTVRDLGSGDMIDVGLRNAARAGHIT
                     GPRMLVAVHGIGATGGHCDPTVGYRADLFDRNSGIDESVANGPDAVRAAVRLNIKRGA
                     DVIKTCATGGVLSMNDDVQSPQLTQEELNALVDEAHARGRKAAAHAHGAEGAKRAVRA
                     GIDSIEHGSFLDDEALELMRQRGTFLVPTLMAAEGLREKLERGSYFPPAVEAKARLAM
                     SQMRATFRKAVQRKVRVAFGTDAAVYPHGRNAEEFRHMVELGMRPSDALRAATSVAAE
                     LFGLSDRLGTLEAGKLADLVAVPGDPTADIRQTERVFFVMKEGVVYKNERR"
     gene            complement(6602..6826)
                     /locus_tag="VF240_02225"
     CDS             complement(6602..6826)
                     /locus_tag="VF240_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174088.1"
                     /translation="MSDADYPTSTQGEGRCPRCGEGAMKAWYELSADEREVVARLPAS
                     AELPLAERAARGHWCTRCWHEDSTGAPRNA"
     gene            6947..>8967
                     /locus_tag="VF240_02230"
     CDS             6947..>8967
                     /locus_tag="VF240_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX7174089.1"
                     /translation="MTQRLARAFFLAASLVITLAAPAAFAQTPSTTQAARDARFDFYE
                     RGPYREQVPRPQSILRFDVGDFHTNYALMERVVGAIAGAAPERVRVFDIGLTNEYRMQ
                     HLVAISSPENIARLDEIKQNMARLADPRTLTPAEAQRLTASTPVIMWMQYTIHGNESA
                     SFEAMMQVVYQLAASDEPATLEILRNSVVLVNVCANPDGHERFVTWYNSFGQGNPDPS
                     AAEHDEPWSVYGRVNRYRFDLNRDNVATSQVETQNMQRAFLEWHPQVAVDHHGQPSQY
                     FFPPAALPVNPNLPPEQTERWLSAFGRANAAQFDARNWDFYVRDIFDLFYPGYWDSWP
                     GLHGATGMTYETDGGGWKGLRWRRDDDTIVTMRSGIAKHFVASMTTLETAARNREARV
                     RDYYEFKRTGIEEGRAGTIKRFVILPGRDPGRAAELVTNLLRAGVEVRRADASFRSTR
                     AHDYSGPNAPAATKDFPAGAFVVDLAQPQKRMAKALLEPDTPQDAAFMREQLARFARN
                     ERRARGANREGYGFYDITAWSLPLVFGVEAYWTEDAAPVGGELLKQPDASDGGRTASG
                     RMGPMLLLEGGVTGRASIAYIIPYERNGAASLIYRLLREDFKLAVATRPLSAGGRDWP
                     RGTVVARVSRNAESLHERIAQLAQQTGTHVTAVNTGFTETGDVAVGSETV"
BASE COUNT         1463 a         3221 c         2926 g         1357 t
ORIGIN      
        1 gcggcagaat gtagcccacg gcgcgagccg tggggacacg cccgattaaa gaatcgaagc
       61 cccggagggg cgaaagaaat tcaggccggg acatcacgcc cgtcaatttt ctttcgcccc
      121 tccggggctt cttatttttc cggcgcgtat tcccacggct cgcgccgtgg gctacattct
      181 atcgccgctt acagcggctc gccgacgcct gcgacttgtc ttcactttat ccttccacct
      241 tcagccttca tccttgcttt agagcacccc gcgaaaccac ttccacgcat aacaggcgac
      301 ccccgcgagt gagtagacgc cgagcgggat gatgatggcg aacttgacgg ccgcgcacca
      361 ccatgcacgt cgcggcagcg tctcgccgtc gaagccgtcg agcatcccgt acatcgcgcc
      421 gttttccacg tagtcgagca ccccggccag ccacggcagc cacgcgacgg cgagggccac
      481 gtcgtaggcg gtcgtccccc tgccgaaaaa cgcccgcgcg gctatcacgc aaccgagcgc
      541 ccacagcgtc gagtagacgg ggatgaggaa ggcgtcccag cgcagatgcc tgtatgcctc
      601 gccgatcatg ccctcacgcg cccacgagtt gatgatctgc tcggccctct cctctgtgcc
      661 ggcgagttcg agcgagacgg cgcgccgcgg cgcctccggc gtcagcagac ggcccttcgt
      721 ccggttgccg atgagcagga agaggacgag caggacggag aaggacgcga gcacgggcca
      781 gccgtcgtgg atggcgttga gggggtgggg tgtgagcatg tcttcaaact ccgcggttag
      841 gacttgaggg acgacgcgcc ggttcggtgt cggaaagtgt gttggctcgc gcgacagcgg
      901 ccgggtgcag gaggacgcgc gcggctgtcg cctcgcgcgt catctgacgg cgcacgggaa
      961 atgcgcgggg aatatatcac gcgcgcggcg gcgggcggta attttgaagg cgctgcccgg
     1021 agggggtaaa ttgatagacg aatgcgaagc gagtcgtcca gcggcgccgg cgaagaagtc
     1081 gatgctctcg tgcgcgagct gcccgacccg gagggggcgc ggctctttta cgagcgcgtg
     1141 gtggccgagc accccccggc gcgccgcctc ttcgagcgcg acgcggggct gctctcggac
     1201 gcgctggcgc tcgccgcgtg gagtccgctc ctcggcacga cgctcgcgca gcagcccgaa
     1261 cacctccagt ggctcgcgcg cgagcgcgtg gcctcgcgcg tgaagacgac ggaggagctg
     1321 ggcgagtcgc tcgcgcgctt cgccctcacg cacacgcagg ccggcacgca ggtaatcctc
     1381 gcgcgcttcc gccggcggga gcttttgcgc gtctacctcc acgacctgcg ccacacgacg
     1441 acgctcgtcg agacgaccga ggagctgtcg aacctcgccg acgccgtctt ggcctacgcg
     1501 ctccaactgg cgcaatcgga gcttgaaaac ctctacggcg cgccgcagtg tacggacgag
     1561 cgtgggcgca agcggagcgc ctcactctgc atcgtcgccc tcggcaaact cggctcgcgc
     1621 gagctgaact actcgtccga catcgacctg ctctttatct actccgaaga cggcgagacg
     1681 agcggcacgg gtgagcgcgg cgccatcacc aaccgcgagt tcttctgcaa gctcgccgag
     1741 ggggtcgcgc gcctcgtggg gcagccgtcg ggcgagggcg cggcctaccg cgtcgatttg
     1801 cgtctcaggc cccacggccg cgacggagcg ctggccgtct cactccgcga ggcgctgcgc
     1861 tactaccacg agacggcgca cgcgtgggaa cttcaggcgc tcatccggca gcgcgcctcg
     1921 gccggctcgc cccagctctt cgcgcgcttc agtgagggat tgcgcgagcg cgtctaccga
     1981 cgcgatgaaa cggtcgcgca cgccctcttc aacgtccgtc tcgccaagca gaagatcgac
     2041 cgcttccacc gacgcgaggc cggcggctac aacgtcaagc tcggtcgcgg cggcatccgc
     2101 gagctggagt tcatcgcgca ggccctccaa ctcgcgcacg gcgggcgcga cccgtggctg
     2161 cacgcgcccc acacgctcat ctccctcagc cgcctcgccg accgccacct cgtcaccgag
     2221 cgcgagacga ccagtctctc cgacgcctac accttcctcc gcatgctcga acaccgcctc
     2281 cagatggagc acggtctgca aacgcacacg ctccccgacg accccgcgcg ccgctccctc
     2341 gtcgcgcgcc gcatgcactt ccacggcgcg tcggccctgg aagagttcga gcaggcgcta
     2401 cgcacacaca tcgcaaacgt ccgcgccgtt tacgaccgcg tcttcggcga tacggaagac
     2461 gacgcggcgc aggcaaccgc gacgactgac gccgcgaccg cgacgccgcg cgtcacggcc
     2521 acgccggccc cggacgcccg cgccccggcg gcgcccgaag cgtcacccgc cccacggggc
     2581 agcgccctcg cccatcccgc gcccgacgac cggggcgcac gcacgccgga ggatgacgct
     2641 gagaaggccg cgcacgccgc cgccgtcgtg ttcgcgccgc acatcgcggg cgctcttgag
     2701 gagacgaccg ggaaggtcgc gcgccgcctg ctcgacgccg cgcgcggctc gctcaatccg
     2761 aagcgcgccc tcgcgctcct cgcgcgcgtc gcctcgtcgc tcgataaatc ggacgccgac
     2821 gtggagctga acctgaacga agaagacctc ggcgctctcg cccgtctctg cggcgggtcg
     2881 gaatttttag gcgagatggt cgccgccaac ccggagttga ttccggcgct cgcggcgcgt
     2941 gccgaggccg tcggagagcg cgaccaccgc gcggtgctgc gcgccgccgt ggacggcgga
     3001 cggagcttcc gtgaggagtt gtcggccctg cgccgcgcgt gggcgcgcct gctcgtcgag
     3061 atcggcgcgc gcgacgtgga gggcgacatc acgccggccg agtcgaaccg gctccagaca
     3121 acgctcgccg ccgcgagcct caacgcggcg ctcctcgtcg cgcggcgcga gatcgcccgc
     3181 caccacggcc ccttcgccgc agggccgcgc ctctccgtgc tcggcctcgg ccgcctcgcc
     3241 agcggcggca tggactacgg ctccgacctc gacatcatcc tcgtctacga ccccaaagtt
     3301 ccgccgccca tcggcggcct ctcgcacgac gagtcctacg cgcgtctgag cgagccgttc
     3361 gcctccgcgc tctcgaacct cacgcgcgac ggctatctct accgcgtcga tttgcgtctg
     3421 cggcccgacg gccgcaacgg ccccgtcgcc gtcggcgcgc gcagcttcgt caactacata
     3481 cgcgagcgcg ccggtgtgtg ggagctgctg gcctacgtca agctgcgcgc ggtggccggc
     3541 gatctcgaac tggggcgcgc cgtcgagact gaggcgcggc gcacggtaca tgaggcggcg
     3601 cggcgcgcgg gggcggaagt gttgcgcgcg gagacgcggc gcgtgcgaga gcgtctggag
     3661 cgtgagcaca cgcgggggca gggaaggcac ggcacagaca tcaagtacgg ccccggcggc
     3721 atgctcgacg tttatttcgc cgcgcgctac ctgcaactgc gcgacgacgt ggcggacgtg
     3781 ggcgacgacc gctcgacgcg cgccacgctc gcgcgcctgc atgagaacgg ctcactcggc
     3841 gaaacagact ttcacgccct cgaccgaggc tacgcgctcc tgcgccgcat cgaccaccac
     3901 ctgcggctgc tcgccggccg ctccacgcgt ctccccgcgg ccccagacca ccccgtcctc
     3961 tcagacctct cccgacgcac gggccacgcc gcgcccgccg ccctcgtctc tgacctcgac
     4021 gcgcacacag cggccgtccg cgccgcctac ctccgcgtcg tcgaaggcta aagtcggcag
     4081 acacaaacca agggcgcgtc cgtcacgact gtaggggcag ggcaagtccg cggcccgcag
     4141 ccgcgaggaa caattaacat ggatgggcag gattaactgg atgtgaagat ttgaaatctc
     4201 aaatctgaaa ttcaatcatc tgttcttcat cctgcccgtc ctgtccatcc atgttaatta
     4261 cctcccgcga cgggggccgc ggaagatgcc cttgccccta cagtcgtgac ggacgccgag
     4321 aggtcttagc aacggactgt tagaagaaaa tgaagaccag ttccccgacc accagaatcg
     4381 cgatgaagat gcccagcacc accttgacga ttgtcttgtt gcgccggccg tggcgaatct
     4441 gcgaagcgcc cgccacgatg ctggcaaagc cgaacgcgat gacgagtccg aagaggccga
     4501 acatgatcgc catctgcttc tcgtcgcccg cgaacctgtt ggtcgagccg gggaggttgg
     4561 cgttcgcgat gatccccgcc acgagcaccg tgatgccgcc catgaagagg acgaggaaag
     4621 cgccgatgac gagcagcacc cacccgagca cgcgcacctg tccacgcgtg agcacgcgcc
     4681 ccttgccgca ttgcgggcac ttcttcaccg acgcgtccgc caccgcgtag tcgcattggt
     4741 agcaggcacg ctccgcgctc ggcgcgtgcc cggccgcgtg tgccggaagc gagccgccgg
     4801 cgcggccggg gaacgacgtt gcgtagaacg gcccgcgccc gcacgcggga cacgcggcga
     4861 ggtgggacgc cgattcgtgg cgacagttgg agcagacgta aaccgattcc atcacacaca
     4921 ttcctccatc tcgctggcct catcgaaatt gacgattcca aaaggcgcgc acgtcatctc
     4981 cgctcgttct tgtagacgac gccttccttc atgacgaaga agacgcgctc ggtctggcgg
     5041 atgtcggcgg tggggtcgcc ggggacggcg acgaggtcgg cgagcttgcc ggcttcgagc
     5101 gtgccgagac ggtcggagag gccgaagagt tcggccgcca cggaggtcgc cgcgcgcagg
     5161 gcgtccgatg ggcgcatgcc gagttcgacc atgtgtcgga actcctcggc gttgcggccg
     5221 tgcgggtaga cggcggcgtc ggtgccgaag gcgacgcgga ctttgcgctg gacggccttg
     5281 cggaaggtgg cgcgcatttg cgacatggcc aggcgcgctt tggcctcgac ggccggcggg
     5341 aagtaggagc cgcgctccaa cttctcacgc aggccctcgg cggccatcag cgtcgggacg
     5401 aggaaggtgc cgcgctggcg catcagctca agcgcctcgt cgtcgaggaa cgagccgtgc
     5461 tcgatggagt cgatgccggc gcgcacggcc cgcttggcgc cctcggcccc gtgcgcgtgg
     5521 gcggcggcct tgcgcccgcg tgcgtgcgcc tcatcgacga gcgcgttcag ctcctcctgc
     5581 gtcaactgcg gcgactgcac gtcgtcgttc atcgagagca cgccgcccgt cgcgcaggtc
     5641 ttgatgacat cggcgccgcg cttgatgttc aggcggacgg cggcgcgcac ggcgtcgggg
     5701 ccgttggcga ccgattcgtc gatgccggag ttacggtcga agaggtcggc gcggtagccg
     5761 accgtggggt cgcagtggcc gcccgtcgcg ccgatgccgt ggacggcgac gagcatccgc
     5821 ggccccgtga tgtggccggc gcgcgcggcg ttacgcaagc ccacgtcgat catgtcgccg
     5881 gagccgaggt cgcgcacggt ggtgaagccg gcgagcaaag tcttccgcgc atggacggtg
     5941 gcgtcgagcg cgcgctcggc cgccgacttc ttcagctcgt cgagttcgtc ctgcttccaa
     6001 tcgtcgctcg actcgcccga cagatgcgtg tgcgcgtcca taaaaccggg caggagcgtg
     6061 gcgtcgccga ggtcgattac ttcggccccc gccgggatag caacgcccgg cccggccgcc
     6121 tgaatcttcc cgcccgacac cacgacgacg ccgggcgaga cgacccgatc actcttgccg
     6181 tcgaacatcc gcgccgcctt gatgacgacg gtctttgcct ccccacgcgc gccgccctgc
     6241 gcgagcgacg ggaggttggc ggcgaagagt gcgaggagca aggcacagac gcagactagt
     6301 ttcatgttta ttctccggca gtggaagttg atacggatta agagcgccta acaaaacgac
     6361 agcggtgagt aattctgtag gggcagggac aagtccgctg cccgccggac acgaaagaaa
     6421 tttacatgga tggacaggac gggcaggata aaaagatttg aaatctgaaa tctgaaatca
     6481 gccctttctt catcctgccc gtcctgtcca tccatgttga ttcctgagtt gagcgggccg
     6541 cggacttgcc ctgcccctac aatcttgtca gacgcttctg gatgtcgttc cctttggggc
     6601 ttcaagcgtt gcgcggcgcg ccggtcgagt cctcgtgcca gcagcgcgtg caccagtgtc
     6661 cgcgcgcggc gcgctcggcg agcgggagtt cggccgaggc gggcaggcgc gcgaccacct
     6721 cgcgctcatc ggcgctcaac tcgtaccatg ccttcatcgc cccctcgccg cagcgcgggc
     6781 agcggccctc gccctgcgtg ctggtcgggt agtcggcgtc gctcatcacg gttgccctcc
     6841 ggcgcggcgt gcgcggcccg ctcggaagat tgagaaacgg ccgagcgcgg gttaatatcg
     6901 cgctgtcttt aacacactcc ccgaagaaaa agaaagctcg cctcgtatga cccaaaggct
     6961 cgcccgcgcg ttcttcctcg ccgcctctct cgtcatcacg ctcgccgccc cggccgcctt
     7021 cgcgcagacg ccgtccacga cgcaggccgc gcgcgacgcg cgcttcgact tctacgagcg
     7081 cggcccctac cgcgagcagg tgccccgccc gcaatcgatc ctccgcttcg acgtgggcga
     7141 ctttcacacc aactatgcgc tgatggagcg cgtcgtcgga gcgattgccg gggccgcccc
     7201 cgagcgcgtg cgcgtcttcg acatcgggct gacgaacgag taccggatgc agcacctcgt
     7261 cgccatcagc tcccccgaaa acatcgcgcg cctcgacgag atcaaacaga acatggcgcg
     7321 tctcgccgac ccgcgcaccc tcacgccggc cgaggcgcag cggctcaccg cttcgacccc
     7381 ggtcatcatg tggatgcagt acaccatcca cggcaacgag tcggcgtcct tcgaggcgat
     7441 gatgcaggtc gtctaccaac tcgcggcgtc ggatgagccg gcgacgctgg agatacttcg
     7501 gaacagcgtc gtcttagtca acgtctgcgc caaccccgac ggccacgagc gtttcgtcac
     7561 gtggtacaac tccttcgggc aagggaaccc agacccctcg gccgccgagc acgacgagcc
     7621 gtggtcggtc tacggccgcg tcaaccgcta ccgttttgat ctgaaccgcg acaacgtcgc
     7681 cacctcgcag gtcgagacgc agaacatgca gcgcgcgttt ctggagtggc acccgcaggt
     7741 ggccgtcgat caccacggcc agccctcgca gtacttcttc ccgccggcgg cgcttcccgt
     7801 caaccccaac ctgccgcccg agcagaccga gcgctggctc tccgccttcg gccgcgccaa
     7861 cgccgcgcag ttcgacgcgc gcaactggga cttttacgtc cgcgacatct tcgacctctt
     7921 ctaccccggc tactgggatt cctggcccgg cctccacggc gccacgggga tgacctacga
     7981 gaccgacggc ggcggctgga agggcctgcg ctggcggcgc gacgacgaca ccatcgtcac
     8041 catgcgctcc ggcatcgcca agcacttcgt cgcgtcgatg accacgctgg agacggccgc
     8101 gcgcaaccgc gaggcgcgcg tgcgcgacta ctacgagttc aagcgcacgg gcatcgagga
     8161 gggacgcgcc ggcacgatca agcgtttcgt cattctgccg ggccgcgacc ccggccgcgc
     8221 cgccgaactg gtcacaaacc tcctgcgcgc gggcgtggaa gtccggcgcg ccgacgcctc
     8281 tttccgctcg acacgcgcgc acgactactc ggggccgaac gcgccggccg cgacaaaaga
     8341 ttttccggcc ggcgccttcg tcgtcgatct cgcgcagccg cagaagcgca tggcgaaggc
     8401 cctgcttgag cctgacacgc cgcaggacgc ggccttcatg cgcgaacagc tcgcgcgctt
     8461 cgcacgcaac gagcggcgcg cgcgcggcgc gaaccgcgag ggctacggct tctacgacat
     8521 cacggcgtgg tcgctgcccc tcgtcttcgg cgtcgaggct tactggacgg aggacgccgc
     8581 gcccgtcggc ggcgagcttt tgaaacagcc cgacgcgtcc gacggcggcc gcacagcctc
     8641 cgggcgcatg gggccgatgc ttctacttga aggcggcgtc acgggccgcg cctccatcgc
     8701 ctacatcatc ccttacgagc gcaacggcgc ggcctcgctc atctaccggc tgctgcgcga
     8761 agacttcaag ctcgccgtcg ccacgcgccc cttaagcgcg ggcgggcgcg actggccgcg
     8821 cggcaccgtc gtcgcgcgcg tctcgcgcaa cgcggagagt ttgcacgaac gcatcgcgca
     8881 gctcgcgcaa cagacgggca cacacgtcac ggccgtcaac accggcttca ccgagacggg
     8941 cgacgtggcc gtcggctctg agacggt
//
LOCUS       DASVES010000059         8177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_527344, whole genome shotgun sequence.
ACCESSION   DASVES010000059 DASVES010000000
VERSION     DASVES010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527344"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VF240_02235"
     CDS             complement(<1..263)
                     /locus_tag="VF240_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7174090.1"
                     /translation="MPGVKKSARLRDLRVGLFVVGAIGVFIFLILNASGDISPFSKKL
                     KLRAQFANGDGLRSGSEVRLAGVRIGKVDKVELLPPAGDRGAKV"
     gene            complement(353..1291)
                     /locus_tag="VF240_02240"
     CDS             complement(353..1291)
                     /locus_tag="VF240_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7174091.1"
                     /translation="MATTNLEVPSKEVHRTDDGPVSEAAAESTFAEVDDHDRVIPAIE
                     FRDVVLSFDERVILDGLSFVVNRGETKVILGGSGGGKSTVINLILGLLKPDSGRILVD
                     GEDITEYTEAEMMGVRKKIGMVFQEGALFDSLSVYDNVAYRLHEQGVPEEEVEQEVRR
                     MLRFVNLEDAIEKMPNELSGGMRRRVGIARALVGGPTIVLFDEPTAGLDPPTARTICE
                     LAIKLRDLEDVSSIFVTHEMNNVGYLTSEYAIVGDDGEVTFEREGERLCLINTKIIML
                     RNGNSIFSGTDEALRKNEDPYIRGFISGHMERKDEG"
     gene            complement(1317..2084)
                     /locus_tag="VF240_02245"
     CDS             complement(1317..2084)
                     /locus_tag="VF240_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7174092.1"
                     /translation="MNIATRALLEVQETTLLAARAARRLFRRPTYLPEMITQMDVIGV
                     GSLTIVLLTGYFTGAVLALQTYPTLKFYGAQDQTGRLVATALVRELGPVLGGLMVAGR
                     VASAIAAELGSMVVSQQIDAMRALGTDPIRKLVAPRLIALVVMLPLLTIIADAVGILG
                     GWTVSTTLYGMLSGTYTAAVWEGLTSKDVVGGFVKALAFAVIIGTVSCRQGLKTEGGT
                     VGVGRSTTTAVVMSSILIIVADFFITKGLQVILGLPT"
     gene            complement(2398..3021)
                     /locus_tag="VF240_02250"
     CDS             complement(2398..3021)
                     /locus_tag="VF240_02250"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX7174093.1"
                     /translation="MKPLLKRACATLRLALCVLILFSAARQALAQSDDDFPQQQQEEG
                     QTRPPRGGDGAGLLRRLNLTPEQVGRIREIRQQSEPEGRALVRRVNQARRALNEAIYS
                     GQADERIVGERARELAEAQAAATRMRAQVEWSIRRVLTDEQLGRLREMRQQAQRTRRM
                     ERRRDRQTPRTSPTDAFAQPPNAPGDANNLPKPRRPLRRRGVGFPNP"
     gene            complement(3058..3855)
                     /locus_tag="VF240_02255"
     CDS             complement(3058..3855)
                     /locus_tag="VF240_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174094.1"
                     /translation="MNNGLGLSDNDHGGRATGQCARGGDLLAYLYNEATVEESRFFTR
                     HLDSCAVCRDELVAFGGVRERVGAWRDEVMSGAPKVDLSGALSQAPATSYDPPARRRR
                     ARVALREFFALSPLWLRAGTATAALVVCALAALVLARTEMRWDDNGVSFRAGVPTRTT
                     VERVEVPAPDMLNAQQADEMARRRVDEALEAQRVEFARELERAVVNARGEAQIANRPA
                     PLMVNDVRQTSRGRSVVRKRPNSRGLEDVRGEEEGLPGLYDLLREAN"
     gene            complement(3852..4499)
                     /locus_tag="VF240_02260"
     CDS             complement(3852..4499)
                     /locus_tag="VF240_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7174095.1"
                     /translation="MSALLFAPGVELTLSGQSLMIETATSDEQMVERALAGEADAFGE
                     IVRRWERKIFGLTFGILGSAEDARDATQETFVSAYRNLRGFRGEAKVSSWLHRIAVNH
                     CVSRQRRAYVRAETALDGQVEARGESFLATPAHASPAAAVEGRERTEAVRRAVCALPL
                     ELREVVVMKEFEELTFQEIADALGVPLSTVKSRLYTALRQLRLRLEKHAAEVAPR"
     gene            4790..6649
                     /locus_tag="VF240_02265"
     CDS             4790..6649
                     /locus_tag="VF240_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7174096.1"
                     /translation="MLERLGRVRLLYIILGTLLVVGLLPLGVAGWMLSERSAEELRSV
                     EGRYQAQLVQDKARQIELFGQRYRDAVTGLASAFELAGGVRVLGDSSSDARLQREVIK
                     DPNLLALAILPVAGTPQVAYRTDLINRDEVDERVGSALSGMSGRGLVIRGPEVIRSSV
                     EMGLTIAAPVMVGGEGGEVVAAVVAVVSFREVFKAVQQNTSLSERELLERGLPVVFVV
                     NPEGRAVAHPDGRVAFSERSMSDLRVVQDWLATGKQLKSALAPFTVEREGQPVAMLGA
                     YATANLDGKSRLGVIAIQDERAALRSVSEMRRQILLISLFAASLALLTGFFFAKQLTR
                     PVHALAEGAHRVAAGDFSQRIDVRGRTAELGDLGESFNKMTDQLQHFIADLQRAAEEN
                     RQLFLGTVKSLAAAIDGKDPYTRGHSERVARFSVAIGERMELPDDEIETLRISALLHD
                     VGKIGIDDNILKKPAALTDEEFEVMKQHPQKGFKIMSHIPAMRDFLPGMYMHHEMIDG
                     RGYPQGLSGDQIPMQARIVSVADTFDAMTTDRPYQQAMKFDDALARIKSFVGTRYDAR
                     VVAALVEACESGQITPGRVRLSKRSKELAASPRLKAASGVGQAAAEPEPTAVA"
     gene            6736..7572
                     /locus_tag="VF240_02270"
     CDS             6736..7572
                     /locus_tag="VF240_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174097.1"
                     /translation="MTRETQLKVLALVAVLFSALSPTAAAQTTRAGKKAATASAQLSR
                     GKNPALHVAPVVNGGETIESIYARNLTGMGGLYALSKINSYIMRGHVIHSLSPIPGAF
                     ETYFKSPGQMLLVMNTPAGQFIQGFDGQKSWIQTPLASASLMGKRPASMLNPASGHGR
                     TRQPGVVYKVKGKTRVGERQADVVEVVIPGETTSTEYYDTTDGLLLRVDTTYKTPDSS
                     EKIRVDIYIDRYAEVNGVKVPIKVRYVLQEATLTVSIYEVKHNVAINDALFSSPTQAS
                     KK"
     gene            7763..>8177
                     /locus_tag="VF240_02275"
     CDS             7763..>8177
                     /locus_tag="VF240_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174098.1"
                     /translation="MGLFWRRKKEDRYITLGLNEPAGAEAKSVPAGPPALEPPADLST
                     GQQPAGLAQPRLEPTPAGDAPTPMPVHEGRAGTPVADSVVETKTPAVESFKTASASTP
                     KPPTQVRTNESARADAATAPAQRPVPPRSSFGTSVL"
BASE COUNT         1484 a         2596 c         2680 g         1417 t
ORIGIN      
        1 accttcgcgc cgcggtcgcc cgcgggcggc agcagttcca ccttgtcaac cttgccgatg
       61 cggacgccgg cgagacgcac ctcggagccg gagcgcaggc cgtcgccgtt ggcgaactgg
      121 gcgcgcagct tcagcttctt cgagaagggc gagatgtcgc cggaggcgtt gaggatcagg
      181 aaaataaaaa cgccaatggc gccgaccacg aagaggccga cgcgcaaatc ccgcagcctg
      241 gccgactttt taactccggg cataatcttt ttgagggatg agggatgagg gatgagggat
      301 gaagagaagg cacatcggcg gcgactcctc aactcctttc atccctcatc cctcatccct
      361 catccttcct ttccatgtgt ccgctgatga agccgcggat gtaggggtct tcgttcttac
      421 gcagagcctc gtccgtgccg ctgaagatgc tgttgccgtt gcggagcatg atgatcttcg
      481 tgttgatgag acagaggcgc tcgccctcgc gctcgaaggt gacctcgccg tcgtcgccca
      541 cgatggcgta ctccgaggtc aggtagccga cgttgttcat ctcgtgcgtc acgaagatgg
      601 aggagacgtc ttcgaggtcg cgcagcttga tggccagctc gcagatggtg cgggccgtgg
      661 gcgggtcgag accggccgtc ggctcgtcga agaggacgat ggtcgggccg ccgaccagcg
      721 cacgggcgat gccgactctc cggcgcatgc cgcccgacaa ctcgttgggc atcttctcga
      781 tggcgtcttc gaggttgacg aagcggagca tgcggcgcac ctcctgctcg acctcctcct
      841 cgggcacgcc ctgctcgtgc aggcggtagg cgacgttgtc gtagacggag agcgagtcga
      901 agagcgcgcc ctcctgaaag accatgccga tcttcttgcg cactcccatc atctcggctt
      961 cggtgtattc ggtgatgtcc tcgccgtcaa cgaggatgcg ccccgagtcg ggcttcagca
     1021 agccgaggat gaggttgata acggttgact tcccgccgcc cgagccgccg agaatgacct
     1081 tcgtctcgcc ccggttgacg acgaagctga ggccgtcgag gatgacgcgc tcgtcgaagg
     1141 agagaaccac atcccgaaac tcgatggccg ggatgacgcg gtcgtggtcg tcaacttcgg
     1201 cgaaggtaga ctcggccgcc gcctcgctga ccgggccgtc gtcagtgcga tggacttcct
     1261 tgctcgggac ttcgagattc gtggtcgcca tacgttcgga accgccggcc agcctctcaa
     1321 gtgggcagtc ctagaataac ctgaagtccc ttggttataa agaagtccgc gacgatgatg
     1381 aggatcgacg acatcaccac ggcggtcgtc gtcgagcgcc ccaccccgac cgttccgccc
     1441 tccgtcttca aaccctgacg gcacgagacc gtcccgatga tgacggcaaa ggcgagcgcc
     1501 ttgacgaagc cgccgaccac atccttggaa gtcagtccct cccacacggc ggcggtataa
     1561 gtgcctgaaa gcattccgta gagcgttgtg gagaccgtcc agccgccaag gatgccgacg
     1621 gcgtcggcga tgatggtgag gagcggcaac atgacgacga gcgcgatgag gcggggggcg
     1681 acgagcttgc ggatggggtc ggtgccgagc gcgcgcatgg cgtcgatctg ctgcgagacg
     1741 accatcgagc cgagttcggc ggcgatggct gaggccacgc gccccgccac catcaaaccg
     1801 ccgaggacgg ggccgagttc gcgcaccagc gccgtcgcca ccagacgccc cgtctgatcc
     1861 tgcgccccgt agaatttcag cgtcgggtag gtctggaggg cgaggacggc gccggtgaag
     1921 tagccggtca gcagcacgat ggtgagcgag ccgacgccga tcacgtccat ctgcgtgatc
     1981 atctcgggca ggtaggtggg ccggcgaaag agccttcgcg cggcgcgtgc cgcgagcagt
     2041 gtggtctcct gtacttcgag cagggcgcgc gtggcgatgt tcatcggtga gttcggaaac
     2101 gagatttccc ggctgaagcg gtagccgccc aagacctcgg caaccgccgc ggagtggacg
     2161 gtgtccggtc gtagggtgac gtggccgcac gtccgccggt atttacaaga tgtagtgcgg
     2221 cctgcgttta aatgcggctc aatgatgcga aaaaaggggc gggcgcgtca aggggaagcg
     2281 gtcagtcgtg aatcgtaaat ggtaaatcgt aaatcgattt agttgagtat ggaagcgccg
     2341 gccgagtctt tggctgagcc ggataacact caatcacgat tgacgattca cggattttca
     2401 cggattgggg aagccgacgc cgcggcggcg cagggggcga cgcggtttgg ggaggttgtt
     2461 ggcgtcgccg ggggcgttcg ggggttgggc gaaggcgtca gtcggagacg tgcggggagt
     2521 ttggcggtcg cgtcgccgtt ccatgcgccg cgttcgttgg gcctgctggc gcatttcgcg
     2581 gagcctgccg agttgttcgt ccgtgaggac gcggcggatg ctccactcga cctgcgcgcg
     2641 catgcgcgtg gcggcggcct gcgcttcggc cagttcgcgt gcgcgctcgc cgacaatgcg
     2701 ctcatcggcc tgacccgaat agatggcctc gttgagcgcg cggcgcgcct gattgacgcg
     2761 ccgaacgagc gcgcgccctt ccggctcgct ctgttgccgt atctcgcgaa tccggccgac
     2821 ctgttcgggc gtcagattca gacgtctcag gaggcccgcg ccgtcgccgc cacgcggagg
     2881 ccgcgtctgt ccctcttcct gctgctgctg cgggaaatcg tcgtcgctct gggcgagcgc
     2941 ctgccgggcg gcggagaaga gaatcaacac gcagagcgcg agccggagcg tcgcgcacgc
     3001 gcgtttgagt aggggcttca tcaattccgg catagactca tccaactact tgtcgtctca
     3061 gttggcctct cgcaaaagat cgtagaggcc cggcagtcct tcctcttcgc cccgcacgtc
     3121 ttcgagacca cggctgttcg ggcgcttcct gacaacggag cgcccgcgcg atgtctgacg
     3181 tacatcattg accatgaggg gagccgggcg attcgctatc tgagcctccc cacgcgcgtt
     3241 gactacagcg cgctccaact cgcgggcgaa ctccacacgc tgagcttcga gcgcctcatc
     3301 gacgcgccgc cgggccatct cgtccgcctg ctgcgcgttg agcatgtcgg gggcgggaac
     3361 ctcgacgcgc tcaacagttg tccttgtcgg aacgcccgcg cggaaagaga cgccgttgtc
     3421 gtcccagcgc atctccgtgc gcgcgaggac gagcgcggca agcgcgcaga cgacaagtgc
     3481 ggctgtggcc gtccccgccc ggagccacag aggagagagc gcgaagaact cacgcagggc
     3541 gacgcgcgcc cgacgcctgc gcgcgggcgg atcgtaggaa gtcgccgggg cctgtgagag
     3601 tgcaccggat agatcgactt tgggcgcgcc actcataact tcatcacgcc acgcgccgac
     3661 cctctcacgg acgccgccga aagcgacaag ctcgtcgcgg cacacagcgc acgagtcgag
     3721 atgccgtgtg aagaatctgc tctcctcgac ggtcgcctcg ttgtagaggt aagccaacag
     3781 gtcgccgccg cgagcgcact ggcccgtcgc gcgaccgccg tggtcgttgt cagataatcc
     3841 gagtccattg ttcatcgcgg ggcgacctcc gctgcgtgct tctccagacg tagacgcaat
     3901 tgcctgagcg ccgtgtagag gcgactcttg accgtgctca gggggacgcc gagggcgtcg
     3961 gcaatctcct gaaacgtcaa ctcctcgaac tccttcatta caaccacctc gcgcaactcc
     4021 aacggcagcg cacaaacggc ccgccgcaca gcctccgtgc gctcccttcc ttcgacggcc
     4081 gcggccggcg aggcgtgcgc cggcgtcgcc agaaagctct cgccccgcgc ctcgacctgt
     4141 ccgtcgagcg cggtctcggc gcgcacgtat gcgcggcgct ggcgcgagac gcagtggttg
     4201 acggcgatgc ggtggagcca ggaggagact ttagcctcgc cccggaagcc gcgcaggttg
     4261 cggtaggccg agacgaaggt ctcctgcgtg gcgtcgcgcg cgtcctcggc gctcccgagg
     4321 atgccgaacg tgagaccgaa aatctttcgc tcccaacgac gcacgatctc cccgaacgcg
     4381 tcggcctcgc cggccagggc gcgctccacc atctgctcgt cgctcgtcgc cgtctcgatc
     4441 atcaagctct ggcccgacag agtcagttca acccccggcg caaacagcag cgcgctcaaa
     4501 cccttagacg cactgctgtt ccggctggtc tgtaaataat ccgaaacgcg cgttcgaggc
     4561 ggcaactcac aaaccggccg cgacgcacat cacgcgagag agtcgaataa tagtcacatt
     4621 cgcccattat tatcaacaag ttaaagttag tttaatattg acacgcggct gttggcaacg
     4681 taatattccc catgtgcgga catcgggttg gtggccgcgc gcggtggagt aaaacttccc
     4741 gccgcccttc cctgaatgtt ttccgtcgtg cctcctgaca gctttcacca tgcttgaacg
     4801 actcgggcgc gtgcgcctgc tctacatcat actcgggacg ctcctcgtcg tggggctgct
     4861 gccgttgggc gtggccggct ggatgctttc tgaaaggagc gccgaggagc ttcgttcggt
     4921 cgaggggcgc tatcaggcgc agctcgtaca ggacaaggca cgccagattg aactcttcgg
     4981 gcaacgctac cgggacgcgg tgacgggact tgccagcgct ttcgagctgg cgggcggcgt
     5041 gcgcgtgctc ggcgactcaa gcagcgacgc acggctccag cgcgaagtca tcaaagaccc
     5101 caaccttctg gcgctcgcca tcctgcccgt ggcggggacg ccgcaggtgg cttacaggac
     5161 tgacctcatc aaccgcgacg aggtggacga acgcgtcggc agcgcgctgt ctggaatgtc
     5221 cgggcgcggg ctggtcatca gggggccgga ggtcatccgt tcgagcgtcg agatggggct
     5281 gacgattgcc gcgccggtga tggtcggcgg cgagggcggc gaggtggtcg cggccgtggt
     5341 cgcggtcgtc tccttccgag aagtcttcaa ggcggtgcag cagaacacgt cgctcagcga
     5401 gcgtgagttg ctggagcgcg ggctgcccgt cgtcttcgtc gtcaacccgg aggggcgcgc
     5461 cgtggcgcac ccggatggga gagtggcctt ctccgaacgt tcgatgtccg acctccgcgt
     5521 cgtccaggac tggcttgcca cgggcaaaca actcaagtcg gcgctcgcgc cgttcacggt
     5581 cgagcgcgag ggccaacccg tcgcgatgct cggggcctac gccacggcca atctcgacgg
     5641 caagtcgcgt ctcggcgtga tcgccattca ggacgaacgc gcggcgctgc gctcggtcag
     5701 cgagatgcgc cggcagattc tcctcatcag ccttttcgca gcgagcctcg cgctactcac
     5761 cggattcttt ttcgccaaac agttgacgcg ccccgtacac gcgctcgccg agggcgcaca
     5821 ccgcgtcgcc gccggcgact tctcgcagcg catcgacgtg cgtggccgca cggcggaatt
     5881 gggcgacctc ggcgagtcgt tcaacaagat gaccgaccag ctccagcact tcatcgccga
     5941 cctccagcgc gcggcggaag agaaccggca gctcttcctc ggcaccgtca agtcgctcgc
     6001 cgcggccatc gacggcaaag acccgtacac gcgcggccac tcggagcgcg tcgcgcgctt
     6061 ctcggtggcc atcggcgagc gcatggaact gcccgacgac gagatcgaga cgctgcgcat
     6121 cagcgcgctc ctccatgacg tgggcaagat cggcatcgac gacaacatcc tgaagaagcc
     6181 cgcggcgctc accgacgaag agttcgaggt gatgaagcag catccgcaaa aaggcttcaa
     6241 gatcatgtcg cacatcccgg ccatgcgcga cttcctgccg ggcatgtaca tgcaccacga
     6301 gatgatcgac gggcgcggct acccgcaggg cctcagcggc gaccaaatcc ccatgcaggc
     6361 gcgcatcgtc tccgtcgccg acaccttcga cgcgatgacg accgaccgcc cctatcagca
     6421 ggcgatgaag tttgacgacg cactggcgcg tatcaagagt ttcgtcggca cgcgctacga
     6481 cgcgcgcgtg gtcgcagccc tcgtcgaggc gtgcgaatcg gggcaaatca cgccgggtcg
     6541 cgtgcgtctc tcgaagcgct cgaaagagct ggcggcgtca ccgcgcctca aggccgcgtc
     6601 cggcgtcggg caggcggcgg ccgagccgga gccgaccgcg gtggcctgaa aaacgatgga
     6661 gacgatgctt cggtagggtg aaaggtctcg ggttggagtt gaggccgcgt cctgaatgtt
     6721 gcgagggata attcgatgac tcgggaaaca cagttgaagg tgctcgcgtt ggttgccgtc
     6781 ctgttcagcg ccctgtcgcc gacggccgcg gcacagacga cgcgcgccgg gaagaaagct
     6841 gcgacggcgt cggcgcagtt gtccagaggt aaaaaccccg cgctgcacgt ggcgcccgtt
     6901 gtcaacggcg gcgagacaat cgagagcatc tacgcgagga atctcacggg gatgggcggg
     6961 ttatacgctc tctccaaaat caattcgtac atcatgcgcg ggcacgtcat ccactcgctg
     7021 tctcccatcc ccggcgcctt cgagacgtac ttcaagagtc cggggcaaat gctgttggtg
     7081 atgaacactc cggcggggca gttcatacag gggttcgacg gccaaaagag ttggattcag
     7141 accccgttgg cgtcggcgag tctgatgggg aagcgtcccg cgagcatgct caacccggcg
     7201 tcggggcacg ggcgcactcg gcagccgggc gtggtctaca aggtgaaggg caagacaagg
     7261 gttggcgaga ggcaggccga cgttgtcgag gtcgtcattc cgggggagac gaccagcacg
     7321 gagtactacg acacgacgga cggcctcctg ctgcgtgtag acacgacata caagacgccc
     7381 gactcgtcgg agaagatcag ggtcgatatt tacatagacc gttacgcgga ggtgaacggt
     7441 gtgaaagttc ccatcaaggt gcgctacgtt ttacaggaag ccacactgac ggtgagcatc
     7501 tacgaggtca agcacaacgt cgcgatcaac gacgcgttgt tttcgagtcc gacacaggcg
     7561 tcgaaaaagt aaggatgagg gatgagggat gagggatgaa aaggaggctt gattcagcct
     7621 tcatttcatc cctcatccct catccctcat ccctgatctt cccccccgac cgccgcactg
     7681 ctttgtcgca gtgcggcggt tccgtctata atcttcaact ttccaacgtg tcggtaattc
     7741 atgcgggggc atcgtctatc tcatgggact tttctggcgt cgaaaaaagg aagaccgtta
     7801 catcactctc ggcctgaacg agccggccgg ggctgaggcc aagtccgtgc cggccgggcc
     7861 accggcgttg gagccgccgg ctgatttatc caccgggcaa caacctgcgg gcctcgcgca
     7921 gccgagactg gagccgacgc cggccggcga cgcgccgacc ccgatgcccg tccacgaagg
     7981 ccgcgccggg acgcccgtcg ccgacagcgt cgtcgagaca aagacgccgg cagtcgagtc
     8041 ttttaaaacg gcatctgcct caactccgaa gccgccaaca caggtaagga caaatgagtc
     8101 ggcgcgcgcg gacgcggcaa ctgccccggc gcaacgtccc gtgccgccgc gctcgtcttt
     8161 cgggacttcg gtgctcg
//
LOCUS       DASVES010000060         4646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_912738, whole genome shotgun sequence.
ACCESSION   DASVES010000060 DASVES010000000
VERSION     DASVES010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912738"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VF240_02280"
     CDS             <1..469
                     /locus_tag="VF240_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174099.1"
                     /translation="STGEDSVFTRVNKYLDRSVARYADHFFESAPTTAREGQVSNSWR
                     LAGKGLLTFLIDLALLPVMFFFLPLRLLMLLLYTPLGLLQERSERKRGSAPAVDGASA
                     SSKRELPEAGGWRVGAVGSVTENTTVHLELAEPTGWQTNASDDIPGKPRITTR"
     gene            545..790
                     /locus_tag="VF240_02285"
     CDS             545..790
                     /locus_tag="VF240_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX7174100.1"
                     /translation="METAERAKVVLYVVPDCPLCARARAWLRRNDVPFVERDVANDFG
                     ALRTMYKLTRQRNVPVFHAESGAFVRPSEEELEAFLL"
     gene            895..1863
                     /gene="miaA"
                     /locus_tag="VF240_02290"
     CDS             895..1863
                     /gene="miaA"
                     /locus_tag="VF240_02290"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX7174101.1"
                     /translation="MTNTPTDKPVIALVGPTASGKSALGIEMALRFGGEIVNCDSVQV
                     YREIEIATAKVPPHERRGVAHHLIDFVPPHVRFSAGEWARRAADVIREIESRGRLALL
                     VGGTGLYLRALREPFFESPPTDERLRSRLLTLRERRGPAHLHRMLLRLDPATAARLAS
                     RDWSRVQRALEVYFQTGLPLSRQRVERPDPPEFARRIRVFALSPPRAELFRRIDERTA
                     SHFDGGLVEEVRGLLARGVPTHSSALGSHGYRRVVEHLRGERDLASAIEQTRLDVRHY
                     AKRQMTWFRREPGVEWVEGFGDEARVEEEVAARIVVALNTNLEAGS"
     gene            2102..2374
                     /gene="hfq"
                     /locus_tag="VF240_02295"
     CDS             2102..2374
                     /gene="hfq"
                     /locus_tag="VF240_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX7174102.1"
                     /translation="MQDAFLNTVRREKDTVTLYLMNGAKLSGRIKSFDKYSVLMESGA
                     HEQLIFKHAISTIQHSRRSGGDPRPHQTQEQPTPQARTEPAAKSEG"
     gene            2420..3079
                     /gene="tmk"
                     /locus_tag="VF240_02300"
     CDS             2420..3079
                     /gene="tmk"
                     /locus_tag="VF240_02300"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7174103.1"
                     /translation="MAAAFITFEGIDGCGKSTQLRMLASELRLRGHEVVATREPGGTS
                     FGQRLREVLLDAQEQVDPLAELLVFAADRAQHVRALVRPALESDHIVLSDRYADATVA
                     YQGAGRGFPEELIADVVALATAGLQPDLTLIFDLAVDESRRRAQKRARKGAAHDRLDL
                     EDVAFHARVREAYLRIAAADPERVRVIDANGSVQETQAAVMQLVMPFIEFKVQSSKFK
                     E"
     gene            3194..4231
                     /locus_tag="VF240_02305"
     CDS             3194..4231
                     /locus_tag="VF240_02305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX7174104.1"
                     /translation="MFDQLAGNQRAKEILRRMLAQGRVPGALLFAGEEGVGKKLFALE
                     LARAFTCRRPSGVEACGECPACVRTGKIEYPAGDDKDAHKKVVWSNHPDVGLILPSNR
                     SVLVDAVRDLERETNFRPVEGRARVFIVENADRLNDSSSNALLKTLEEPHATSHLVLL
                     TSRPASLLPTIRSRCQVVRFSPLSVEEIEQHLVKAHKRAGQEARLAAQLARGRLGAAL
                     SIDLDAYRERREQMLGVLDALAVSGDRSRLLRAAEQLGDAKNKDAYEPSLDLLETLIR
                     DLWLLALKAPDARLVNEDTRERLARMGAHLRPARASRWLSRVEELRAQLAVNVNRKAA
                     TDALFLAMASD"
BASE COUNT          834 a         1618 c         1360 g          834 t
ORIGIN      
        1 ctcaaccggc gaagacagtg tattcaccag agtcaataag tatctcgacc gctccgtcgc
       61 acgctacgcc gaccacttct tcgagagtgc tcctacgacg gctcgcgagg gtcaggtcag
      121 caattcatgg cggctcgcgg gtaaagggtt gctcactttc cttatcgatc tcgccttgct
      181 cccggtgatg tttttctttc tccccttaag gctcctgatg ctgttgttgt atacgcctct
      241 gggtttgttg caagagcgga gtgagcgaaa gcgaggaagc gcgcctgctg tcgatggggc
      301 gagtgcatca tccaagcgag aattgccgga agcagggggc tggcgggtcg gcgcggttgg
      361 cagtgtgact gaaaatacaa cggtgcattt agaacttgcc gaacccacag ggtggcagac
      421 caacgcttct gacgatatcc ctggcaagcc gcgcataaca accagatgaa ccggactcgc
      481 tcccggcaca cctgctaaag gtcggagttt atgctcgccg gttatctgga gcgttaggca
      541 tcttatggag acagctgaac gagcgaaagt tgttctgtac gtcgtgcccg attgcccgct
      601 ctgcgcccgc gcgcgcgcgt ggctgcggcg caacgatgtg cctttcgtcg agcgcgacgt
      661 ggccaacgat ttcggggcgc tcaggacgat gtacaagctg acccgccagc gcaacgtgcc
      721 ggtcttccac gccgagtcgg gcgcgttcgt ccgcccgtcc gaagaggaac tcgaagcgtt
      781 cctgctctga accgcaatgg ccctccgctt cacacgcgca caacttctcg ccgcgctcgc
      841 gctgctcctg ctcctctggc tcatcgtcgc ctttcgcgtc ttcgccgcac gcccgtgacc
      901 aacaccccga ccgataagcc cgtcatcgct cttgtcggcc ccaccgcctc cggcaagagc
      961 gccctcggca tcgagatggc cctgcgcttc ggcggcgaga tcgtcaactg cgactccgta
     1021 caggtctatc gcgaaatcga aatcgcgacg gcgaaagtgc cgccccacga acgacgcggc
     1081 gtagcgcatc acctcatcga cttcgtcccg ccccacgtcc gcttcagcgc cggagagtgg
     1141 gcgcgccgcg ccgccgacgt aattcgggaa atcgagtcgc gcggccggct cgcccttctc
     1201 gtcggcggca ccggcctcta cctgcgcgcg ctgcgcgagc cgttctttga aagcccgccc
     1261 acggacgagc gtctgaggtc gcgcctcctc acactgcgcg agcgccgcgg cccggcgcac
     1321 ctgcacagaa tgcttctgag actcgacccc gccaccgccg cgcgtcttgc gtcccgcgac
     1381 tggtcgcgcg tccagcgcgc cctcgaagtc tatttccaga cgggcctccc cttgagccgg
     1441 cagcgcgtcg aacgacccga cccgcctgag ttcgcccgcc ggatacgcgt cttcgctctg
     1501 agtccgccgc gtgctgaact ctttcgacgg atcgacgagc gcaccgcctc gcacttcgac
     1561 ggcgggttgg tcgaagaagt tcgaggactg ctcgcccgcg gtgtacccac ccattcgagt
     1621 gcgctcggct cgcatggcta ccggcgagtt gtggaacacc tgcggggcga gcgcgacctc
     1681 gcgagcgcca tcgagcagac acgtctcgac gtgcgccact acgcgaagcg gcagatgaca
     1741 tggttccgcc gcgagccggg cgtcgaatgg gtcgaagggt tcggcgacga agcgcgcgtt
     1801 gaggaggagg tcgccgcgcg gatagtcgtc gccctcaaca cgaatctcga agcagggagc
     1861 taggggcttt acctgtccgc gcttccgcgc gacgcgcctt ccgcactcgc acacatctca
     1921 ccccctcgtt acctacacgc tgaccaacat ccacagcgta aatcgctgcg cgacgaactc
     1981 gtctcaaact ttgaaccttg aactttgaac tttgaacttg tactcttctc tcactcggat
     2041 aaaaacattc ccgagaacgg gcggggtgac agcagcagtg gaaaccaaat ctccacagaa
     2101 catgcaggac gcgttcctga acacggtgcg ccgtgagaag gacacggtga cgctctacct
     2161 gatgaacggc gcgaagctct ccgggcgcat caagagcttc gacaagtatt ccgtcctcat
     2221 ggaatcgggc gcgcacgaac agctcatctt caagcacgcc atctcgacca tccagcactc
     2281 gcgccgctcg ggcggcgacc cccggccgca tcagacccaa gaacagccca cgccccaggc
     2341 gaggacggag ccggcggcca agtcggaagg ctaaggccgg ccgagttcac ctaaacacac
     2401 ttcacggagg caaccccgtt tggccgcggc attcatcacc ttcgagggca tcgacgggtg
     2461 tggcaagtcc acgcaactca ggatgctcgc cagcgaacta cgcctgcgcg gccacgaggt
     2521 cgtcgccacg cgcgagccgg gcggcacctc cttcggccag cgactgcgcg aagtgctcct
     2581 cgacgcgcag gagcaggttg acccgctggc ggagctgctc gtcttcgccg ccgaccgcgc
     2641 gcagcacgtt cgtgccctcg tccggccggc gctcgagtct gaccacatcg tcctctccga
     2701 ccgctacgcc gacgcgacgg tcgcctatca gggcgccggc cgcggcttcc ccgaagaact
     2761 gatcgccgat gtcgttgctc tggctaccgc cggcctccag cccgacctca cgctcatctt
     2821 cgatttggcc gtggacgaga gccgccgccg cgcccagaag cgcgcccgca agggcgccgc
     2881 gcacgaccgc ctcgatctcg aagacgtggc cttccacgcg cgcgtgcgcg aggcgtatct
     2941 ccgcatcgcc gccgccgacc ctgagcgcgt ccgcgtcatc gacgccaacg gctccgtaca
     3001 ggaaactcag gccgccgtga tgcagctcgt catgccgttc atcgagttca aagttcaaag
     3061 ttcaaagttc aaagaatgac cgtctttcga ctttgaactt ctgccttcag gaattccgcc
     3121 tttgaacttc tgcctttcgg atttccgact ttgaactttg aacttggaac tttgaacttc
     3181 tttttttgaa ctcatgttcg accagctcgc aggcaaccaa cgcgcgaagg agattttgag
     3241 acggatgctc gcgcaggggc gcgtccccgg cgcgctgctc ttcgccggcg aggagggcgt
     3301 cggcaagaag ctcttcgcgc tggagctggc gcgcgccttc acctgtcggc ggccctccgg
     3361 cgtcgaagcc tgcggggagt gcccggcgtg cgtccgcacg gggaagatcg aatatccggc
     3421 gggcgacgac aaagacgcgc ataaaaaagt tgtctggagt aaccaccccg atgtcggttt
     3481 gatactgcct tccaaccgct cggtcttagt cgatgccgtc cgcgaccttg aacgcgagac
     3541 caacttccgg ccagtcgagg gccgggcgcg cgtctttatc gtcgagaacg ccgaccgcct
     3601 gaacgactcg tcttccaacg cgctgttgaa gacgcttgaa gaacctcacg cgacctcgca
     3661 cctcgtcctg ctcacgtcgc gcccggccag cctgctgccc acgatccgct cgcgttgtca
     3721 ggtcgtgcgc ttctccccgc tctcggtcga ggagatcgag cagcacctcg tcaaagcaca
     3781 caagcgcgcg gggcaggagg cgcgactcgc ggcgcagctc gcgcgcggcc gtctcggcgc
     3841 cgcactctcc atcgacctcg acgcctaccg cgagcgccgc gagcaaatgc tcggcgtgct
     3901 cgacgcgctc gccgtctcgg gcgaccgctc ccgcctgttg cgcgccgccg aacagttggg
     3961 cgacgcgaaa aacaaagacg cctacgagcc gagtctcgac ctgctcgaaa ccctcatccg
     4021 cgacctctgg ctgctcgccc tcaaggcccc tgacgcgcga cttgtcaacg aggacacacg
     4081 cgagcgcctc gcccggatgg gcgcacacct tcgccccgcg cgcgcctcgc gctggctctc
     4141 ccgcgtcgag gagctgcgcg cgcagcttgc cgtcaacgtc aaccgcaagg cggccaccga
     4201 cgccctgttt ctcgcgatgg cttccgactg acgccgagca cattagatta attgtaactc
     4261 gccaaccagc cccggagggg cggaagaatg tagcccacgg cgtaagccgt gggaacgatc
     4321 cggataagta aagcgaagcc ccgaaggggc gaaagaacct caacgagcaa cgtgctctag
     4381 cttgaagttc tttcgcccct ccggggcttc aatgcgttcc ggcaactgtt ccccggtctc
     4441 gcgaccgggg ctacattctt ccgcccctcc ggggcttatt gaaacttacc aacttcactt
     4501 aggttatctg cacgagttcc ggctgaggct cctctgacac cggtacgcct tcggcgagga
     4561 gcttttccgt ctcggccacg aacgcctcga cgatgtccgt gccggcgacc ttccgcatgg
     4621 gccgaccgtt cttgaagatc atcccg
//
LOCUS       DASVES010000061         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_912016, whole genome shotgun sequence.
ACCESSION   DASVES010000061 DASVES010000000
VERSION     DASVES010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912016"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VF240_02310"
     CDS             <1..458
                     /locus_tag="VF240_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174105.1"
                     /translation="RLLRNLGSIQRIAQAGVAELTPFVGAKTAAEIVEHFTRQRALAE
                     SGGAASSAATAPEQADLPDESVPRARPGGDDPTVMTDETIGDEHIETRLVDPEGDAED
                     LQPIRSVDHAGELRKPRRPTRKRGERSTNPHGVKRERLTDADTEAPDKS"
     gene            727..1113
                     /locus_tag="VF240_02315"
     CDS             727..1113
                     /locus_tag="VF240_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174106.1"
                     /translation="MNRFRRIQDEFGVGALLRTAVLLLGLFGMALFDYFAFSSIRASL
                     IAVVGLLLGFLFRRKIAGGVEYYPRVLSAGLFVSSITLLLGDLLGLDRGVKLAVITAT
                     TVIVFGLQFWSLSDPSVVNAKRNRHA"
     gene            1222..1722
                     /locus_tag="VF240_02320"
     CDS             1222..1722
                     /locus_tag="VF240_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174107.1"
                     /translation="MAKSGYKGVSRIDSPAKKMHGWYVRVRFDNTTRAKFLSDKQHGG
                     REAALLKAVECRNELERELGKPRTDCVIVGSNPRNATGVTGVRRTAKKHHGKDGKIYL
                     NEVYEVTWNAGREKKGRTSVSIKKYGEAAAFRRACGIRRRMEQFMYGAPVEAKWVETL
                     SKLCAA"
     gene            complement(1799..>2351)
                     /locus_tag="VF240_02325"
     CDS             complement(1799..>2351)
                     /locus_tag="VF240_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174108.1"
                     /translation="KLGEGKEALVLDGRAFAYDEIKNAEFGPGGRRFIFKARRAQGWV
                     MVLDGVESETYEADGGAPGEVEWSSEGELTFSRDGGRVAYVARKGGKDFVVVDGVAGA
                     RYDDIANLAFTPDGRHVVHTARRGGKSLVVVDGVEGREYDGFVPAQKHEKEGKLGVEG
                     ERKLSIIATRGAELLLVEIEIAD"
BASE COUNT          425 a          798 c          707 g          421 t
ORIGIN      
        1 accgtctgct ccgcaacctc ggctccatcc agcgcatcgc gcaggccggc gtcgcggagc
       61 tgacaccctt cgtcggcgcc aagacggccg ccgagatcgt cgaacacttc acacgccagc
      121 gcgcgcttgc cgaaagcggc ggcgccgcgt cctccgccgc caccgcgccc gaacaggccg
      181 atctgccgga tgagtcggtg ccgcgcgctc gccccggcgg cgacgacccg accgtgatga
      241 ccgacgagac catcggcgac gagcacatcg aaacgcgcct cgtcgatccc gaaggcgacg
      301 ccgaagacct ccagcccatc cgctccgtcg atcacgccgg cgaactccgc aagccccgcc
      361 gccccacacg caagcgcggc gaacgctcca ccaaccccca cggcgtcaaa cgcgagcgcc
      421 tcaccgacgc cgacacggaa gcgcccgaca aatcgtgacg gcgcggggta cggcaaaagg
      481 cgtcggcccg cgtgtttgta ctgcacgatg cggtaattcc catctggcaa gatgaaatca
      541 ccgcatctcg ttttatacga tgagtagtcg ccaataaaat atggtaatgc ctattttcgg
      601 aacgcatcta acacctcatt caacctgaga cctcgatagc atctctctca tgatgcttcc
      661 ttcggtgtat cttgtgtgcg gcttgctcgg cgcgggtcga ttcgggcatg aggcgaggtc
      721 tcgcgtatga accggtttcg gagaattcaa gacgagttcg gtgtcggggc gttgctccgc
      781 acagccgtgt tgctgctcgg gcttttcggg atggcgcttt ttgactattt cgccttttcg
      841 agtatcaggg cgagcctcat tgccgtggtc gggttgctgc tcgggttcct gttcaggcgg
      901 aagatcgccg ggggagtcga gtactatcca cgcgttcttt ccgccggcct cttcgtgtcc
      961 tcgatcacac tgcttctcgg tgacctgctc ggcctcgaca gaggtgtgaa gctggccgtg
     1021 attaccgcga caacggtgat cgtcttcggc ctacagttct ggtcgctctc ggacccgtcg
     1081 gtagtgaatg cgaaacggaa ccgccacgca tagcagcagt ttgcagctcg tgctttgaca
     1141 aaagcggccg ccgcgtctta acttcatttc agcacgttaa taaatttcat tcagggagac
     1201 tcttttcagg acacgaatcc tatggcgaag tctgggtaca agggtgtaag ccgcatcgac
     1261 agccccgcga agaagatgca cgggtggtac gtccgcgtca ggttcgacaa cacgacgcgc
     1321 gcaaaatttc tctccgacaa gcagcacggc ggccgggagg cggcgctcct gaaggcggtc
     1381 gagtgccgca acgagctgga gcgcgaactc ggcaagccgc gcaccgattg cgtgatcgtc
     1441 ggcagcaacc cgcgcaacgc gacgggcgtg acgggcgtgc gccgcaccgc gaagaagcac
     1501 cacggcaagg acgggaaaat ttatctcaac gaagtctacg aggtgacgtg gaacgcgggc
     1561 cgggagaaga aggggcgcac ctcggtctcc atcaaaaagt atggcgaggc cgcggccttc
     1621 cgccgggcgt gcggcatccg ccggcgcatg gagcagttca tgtacggcgc gccggtcgag
     1681 gccaagtggg tcgagacgct cagcaagctc tgcgcggcct gacgcgcaac cgaacactca
     1741 actctcggcg cgggcgtcgc gccgcccgcg ccgggccgcg tcgctctcgc ctccgtgctc
     1801 aatcggcgat ctctatctcg acgagcaaaa gctccgcccc gcgcgtggcg atgatgctga
     1861 gcttgcgctc tccctcgacg cccagcttac cttccttctc atgcttctgc gcgggcacga
     1921 agccgtcgta ttcgcggccc tcaacgccat cgacgaccac taaagacttg ccgccgcgcc
     1981 gcgccgtgtg gacgacgtgt cggccgtcag gcgtgaaggc gaggttggcg atgtcgtcgt
     2041 agcgcgcgcc cgccacgccg tcaaccacca cgaagtcttt ccctcccttg cgcgcaacgt
     2101 aggccacgcg gcccccgtcg cggctgaagg tgagttcgcc ctcgctgctc cactcgacct
     2161 ctccgggcgc gccgccgtcc gcctcgtaag tttccgactc cacgccgtcc agaaccatca
     2221 cccacccctg agcgcgccgc gccttgaaga tgaagcggcg tccgccgggg ccgaactcgg
     2281 cgtttttgat ttcgtcgtaa gcgaacgcgc ggccgtccaa gaccagcgcc tctttgccct
     2341 cgcccagttt g
//
LOCUS       DASVES010000062         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_718504, whole genome shotgun sequence.
ACCESSION   DASVES010000062 DASVES010000000
VERSION     DASVES010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718504"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1105)
                     /gene="aroF"
                     /locus_tag="VF240_02330"
     CDS             complement(86..1105)
                     /gene="aroF"
                     /locus_tag="VF240_02330"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7174109.1"
                     /translation="MLIVMKRDATERDIEAVVRVINDMGFRAHQLPGENRTAIGVTGN
                     PGSIDPTHFENMAGVAEAIRVTKPYKLISLDLRPEKTVVRVGDATIGGDELAIIAGPC
                     AVESRAQTLAVAEAVRRSGARFFRGGAFKPRTSPYAFQGLGEEGLKILAEVRDTYGLK
                     IVTEAMDERGLELVEKYADVIQLGARNMQNFSLLKRAGHSRLPVLLKRGLSATLDEWL
                     LAAEYVMAEGNYNVILCERGIRTFAQHTRNTLDLAAVPAVRRLSHLPVIVDPSHGTGK
                     SYMVAALARAGVAVGADGLIIEVHDQPDHALCDGAQAITLDAYAQLASEVRAIHEVIT
                     PTPVV"
     gene            complement(1240..>2880)
                     /locus_tag="VF240_02335"
     CDS             complement(1240..>2880)
                     /locus_tag="VF240_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEX7174110.1"
                     /translation="HLRNAVLGDTFVRVLRATGQRVEVENYIDNTGVQVADVVVGFMH
                     IERMTLDDIRALDASLPPERPFDYYCWDLYARVGLFYRDGDPDAKEDPERIALRRQTL
                     HDIEHGASPTAEIADYVATRVVNCHLDTTERIGVRYDVLPRESEILHFVWGRAFERLK
                     ESGSIRLEAEGKNAGCWVMPMDTHTSTDEHEADKVIVRSNGTITYTGKDIGYQLWKLG
                     QLGVDLDYVPFRTYDDGHVVWATTSTPAAGDDAGERPHFGGGQKVYNVIDSRQSYTQD
                     VVRKGVAAVSPEVSEDASVHLAYEMVALSPAAAQELGIELNEEDRARSFIEMSGRRGL
                     GVKADDLIDRLEANALREVESRHPDLSSEEKMRTAHILAVGALRYFLLKWTRNSLIVF
                     DFDEALSTKGEAGPYCQYAAVRANSIFRNLPTEEPEEAARLIASAAAAGGEEAAGVRK
                     VFEGEVGDEIWSLVTLAARLEDVVAQAASAAEPSHLAKYAFQLAQAFNFFYHRRENRI
                     LEEKDTVRRAVLVAVADYVRRRLTAALATLGIEVPERM"
BASE COUNT          472 a          912 c          969 g          527 t
ORIGIN      
        1 cagagggttg aatgtcattc aaccctctgc gccccctctg cgtcctctgc gcctctgcgg
       61 tgaatcacct gtttcgataa atcaactaaa ccaccggcgt cggcgtgatg acctcgtgga
      121 tggcgcgcac ctcggaggcg agctgggcgt aggcgtcgag cgtgatggcc tgcgcgccgt
      181 cgcagagcgc gtggtcgggc tggtcgtgga cttcgatgat gaggccgtcg gccccgacgg
      241 cgacaccggc ccgcgccagc gccgcgacca tgtagctctt gcccgtcccg tgtgagggat
      301 cgacgatcac cgggaggtgc gacagacggc gcacggcggg gacggccgcg aggtcgagcg
      361 tgttgcgcgt gtgctgcgcg aaggtgcgga tgcctcgctc gcagaggatg acgttgtagt
      421 tgccctcggc catcacgtac tcggcggcga gcagccactc gtcgagcgtg gccgatagcc
      481 cgcgcttcag caggacgggc agacgtgaat gaccggcgcg cttcagtagc gagaagttct
      541 gcatgttgcg cgcgccgagt tgaatcacgt cggcgtattt ctccaccagc tccagcccgc
      601 gctcgtccat cgcctcggtg acgatcttca gcccgtatgt gtcacgcacc tcggcgagaa
      661 tcttcaggcc ctcttcgcca agaccctgaa acgcatacgg tgaagtgcgc ggcttgaagg
      721 cgccgcctcg gaagaaacgc gcgccgctgc ggcggacggc ctcggccacg gcgagcgtct
      781 gcgcgcggct ctccacggcg caggggccgg cgatgatggc cagctcgtcg ccgccgatgg
      841 tcgcgtcgcc gacgcgcacg accgtcttct ctggccggag gtcgagagag atcagcttgt
      901 aaggcttggt gacgcggatg gcctcggcca cgcccgccat gttctcgaaa tgtgtggggt
      961 cgatggagcc ggggttgccg gtgacgccga tggccgtgcg gttctcgccc ggcaactggt
     1021 gggcgcgaaa ccccatgtcg ttgatgacgc gcacgaccgc ctcgatgtcg cgctccgtgg
     1081 cgtctctttt catcacaatg agcatcgttg acctcttgag tcggcttcgg cgaaataata
     1141 gctagagtca gcccgctgga aagcgtcggg catcaccgtc tcgttaaaag ataatggcaa
     1201 gacacgtgcc gccgatgaat atgacgacag gcatgacgtc tacatccgtt cggggacttc
     1261 gatcccgagc gtggcgagcg ccgccgtcag acgccggcgc acgtaatcag cgaccgcgac
     1321 gagcaccgcg cgtctcaccg tgtccttctc ttcgaggatg cgattctccc gccggtgata
     1381 gaaaaagttg aaggcctgcg ccaattgaaa cgcatatttg gcgaggtgcg acggctcagc
     1441 ggcggacgcc gcctgcgcga ccacgtcttc gagccgcgcc gccagcgtga caagcgacca
     1501 aatctcgtcg ccgacctcgc cctcgaaaac tttcctcacc ccagccgcct cttcgccgcc
     1561 ggctgcggcc gcggaagcaa ttagccgggc tgcctcttca ggctcctcag ttggcaaatt
     1621 gcgaaagatt gaattggcgc gcacggccgc gtactggcag tacggcccgg cctcgccctt
     1681 cgtcgaaagg gcctcgtcaa agtcgaagac gatgagggag tttcgcgtcc acttcaggag
     1741 gaagtagcgt agcgcgccga cggcgagaat gtgcgccgtc cgcatcttct cctccgacga
     1801 gagatcgggg tggcgcgact cgacctcgcg gagcgcgttc gcttcgaggc ggtcgatcag
     1861 gtcgtcggcc ttcacgccaa gtccgcgccg cccgctcatc tcgataaagc tgcgcgcgcg
     1921 atcctcttcg ttcaactcga tgccgagttc ttgagccgcc gccggcgaga gcgcgaccat
     1981 ctcgtaggcg aggtggacgc tcgcgtcctc actaacctca ggcgagacgg cggcgacgcc
     2041 cttgcggaca acgtcctgcg tgtaagactg tcgcgagtcg atgacgttgt agaccttctg
     2101 gccgccgccg aagtgcggac gctcacccgc gtcgtcgccc gctgcgggcg tgctggtcgt
     2161 cgcccagacg acgtggccgt cgtcgtaagt gcggaagggc acgtagtcta aatccacccc
     2221 gagctggccg agcttccaga gttgatagcc gatgtctttg ccggtgtagg tgatggtgcc
     2281 gttggagcgg acgatgacct tgtcggcctc gtgctcgtcg gtgctggtgt gcgtgtccat
     2341 cggcatcacc cagcagccgg cgttctttcc ctcggcctca aggcggatcg agccagactc
     2401 tttcagacgc tcgaaggcgc ggccccagac gaagtggaga atttcggatt cgcgcgggag
     2461 cacgtcgtag cggacgccga tgcgctcggt cgtgtcgagg tgacagttga cgacgcgcgt
     2521 ggcgacgtag tcggcgatct cggccgtcgg gcttgcgccg tgctcgatgt cgtggagcgt
     2581 ctgtcgtcgc agggcaatgc gctcggggtc ttccttcgca tcggggtcgc cgtcgcggta
     2641 gaagaggccg acgcgcgcgt agaggtccca gcagtaatag tcgaaggggc gctcaggcgg
     2701 gagcgaggcg tcgagggcgc ggatgtcgtc gagggtcatg cgctcgatgt gcatgaagcc
     2761 gacgaccacg tccgccactt gcacgccggt gttgtcgatg tagttctcga cctcgacgcg
     2821 ctgccccgtg gcacggagca cgcggacgaa ggtgtcgccg agcacggcgt ttcgcaggtg
//
LOCUS       DASVES010000063         6429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_930448, whole genome shotgun sequence.
ACCESSION   DASVES010000063 DASVES010000000
VERSION     DASVES010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930448"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..736
                     /locus_tag="VF240_02340"
     CDS             89..736
                     /locus_tag="VF240_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7174111.1"
                     /translation="MRSARSLPPESRPAQRAAPSARAAVADKREAILRAATGVFAKNG
                     YFNSKVADVARVAGVADGTVYLYFKSKEDILHSIFERNTSEAIAEGREELAKIADPRE
                     RLRRIARHHLERLGSDRDLAIVFQVELRGSTKFMEEFSAAGLAEYLGLIREVFEEGQR
                     DGVFRRELNAKLVAKVIFGALDEMATNWILSKRRYKLAPMADEVLEIFFDGVIAR"
     gene            733..2652
                     /locus_tag="VF240_02345"
     CDS             733..2652
                     /locus_tag="VF240_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7174112.1"
                     /translation="MTDTRTTTTTTATEAAPAAATTARAGHAPLPADEPRTLVEVCEH
                     ALRARARPDAFNYKRGDAWLAISSGEFALRVRAIALGLHALGVGRGDRVAILSESSPE
                     WVMADVGCQFAGVVDVPIYPTLAAPQGCYIINDSGSVLLFVQNPAAYERVREVARGCA
                     PLRHVVILEGEAEAGDGDDPAVLTLAQLEDRGRALDRERPGLFDELAGAVGAEDLATI
                     IYTSGTTGEPKGVMLTHSNVVSNLVDTSARLAYTPEDVVLSVLPLSHIFERGAMYMYI
                     YHGTAIYFAESIERIGDNMREVRPTIVVAVPRLFEKIYARIKERAAGGGRAKTSVLMW
                     AVSVAKRWARARSERERVPALLGLQHKLATRLVFSKWQEGMGGRIRVFCSGGAALPEE
                     LGYIFLGAGLPIVQGYGLTETSPVICVNSVADNRIGTVGRAIDHVEVRIAADGEIETR
                     GPNVMRGYYNKPEETEKVFTEDGWFKTGDIGALDRDGYLRITDRKKELFKTSGGKYIA
                     PQPIEQRIKQSRFVNQVVLIGNGRKFAAALIVPDWELLRSYAQHKGIDAATPADLCRH
                     PRVVDLLQRQVDAVTADLSNYERVKRIALIEHELTVAGGEMTPTLKVKRRVVDEKYKD
                     TIDRLYEEAETRPYR"
     gene            2791..3900
                     /locus_tag="VF240_02350"
     CDS             2791..3900
                     /locus_tag="VF240_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX7174113.1"
                     /translation="MADALDIAILDAQAKKRAAVVLARYGSDIAIAELPAGKELKALL
                     DFKDRISQVLLLSQKRPTKKELTEFGHALFAYIVRENVERLYNRLPVNTLIRFHILAN
                     RPDLQSLPWEYLQDPQQGAGPWPNRSIVRVIPTIGYTPPAPLQLAQLAATGTKLRILF
                     VYAVPQDQNLVSWPSVKSSIEKAFSLRIPASHYELKVIQGTTEELFAAFHNNNDKYEI
                     FQFSGHGDVDPQTNEGRILLVNSKKNDQSLPMSASQLASILQSRGVRLAVLSACLTSA
                     GNSADPFNVVAEALLASGIPAVVANHLPVPDASVATFVGALYTQLLNTGDIDIAVNEG
                     RLKMAIELAVPQDATLEWGIPTLYRYINGAQVFQP"
     gene            3897..4955
                     /locus_tag="VF240_02355"
     CDS             3897..4955
                     /locus_tag="VF240_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174114.1"
                     /translation="MKLSPFTLVYQPAREARVLSSRRAATGVQGVGRVSELDARPEAV
                     RELAPDLAGALNSFDPNLARTLIASTYAHESAAPDDPVEEKLWRVVTDMRRAEKVVTV
                     AVWDLDARVGGLGRLVNALNEAQPVFTFFDLQAPLPAGLVISSEGFAEWARKRLEKRV
                     SKSQREGFTDNFMFDDFNKFARVVHKQVGTDYLVGITRHMVAWTQGESVYWNYFTASD
                     RRVILTSAYNLREYAALAGRPFEAAVAGLVVAEILAALNRRLGYHEENRGCLFDFNQE
                     RDTVIESIRQARIEPQCLDLIDEKYRDAAAALTDVLRNYSPPQPDEVPTEKSAKKKAG
                     QDDEYWLAQLTKLSKKTK"
     gene            4991..6340
                     /locus_tag="VF240_02360"
     CDS             4991..6340
                     /locus_tag="VF240_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174115.1"
                     /translation="MTHAAIIIPGIMGSVLELNGEVIWPGAAWEIYLPYRKMDKLLDP
                     NLVATDVIRKVSISTQYQTLIDDLGKCGFRESDNPRTLYVCPYDWRKDNTEAAAKLAA
                     LADQAVTQGGATSLTLIAHSMGGLVSRYFLESGLYAGKPGLKAVRRLLTLGTPHRGAP
                     LALSAALGKEKRLFLNADQVLQVASDPRYPALYQLLPPPGEPFAWDEDKAAEYGDVDI
                     YDAGVAKTLKLVAKSLKAAKDFHASLDISKRPQFDGKPIRYFFFSGTRQTTITSVNVL
                     KVDATRYEVRKTELEDAGDGTVPIWSAAMTGVQGQPVGGEHGTIYRNDTLRRTMAVLL
                     GAKGVLAVAPVRVEVALRERVVHDGDTVHAALTFGAGVDKLDGKLAIHLVEVDAGGGV
                     KSRRLVSEHDISYKGLNAEKLNVIFSAPAFPGIYQVTYVPAGHTDPAGSDELIVQES"
BASE COUNT         1157 a         2300 c         2017 g          955 t
ORIGIN      
        1 gcgaggttag gctaggcgta ctgaatggct attcattcag acgctcctcg gccgcgaaat
       61 cttctgacgg agactgaaga cgaccccgat gcgatccgcc cgctccctgc cccccgaatc
      121 ccggccggcc cagcgcgccg ctccctcggc gcgcgcggcc gtcgccgaca agcgcgaggc
      181 catcctgcgc gccgccaccg gcgtcttcgc caaaaacggc tacttcaact cgaaggtggc
      241 cgacgtggcg cgcgtcgccg gcgtcgccga cggaacggtc tacctctact tcaagagcaa
      301 ggaggacatc ctccactcca tcttcgagcg gaacacctcc gaggccatcg ccgaagggcg
      361 cgaggagctg gcgaagatcg ccgacccgcg cgagcgcctg cgccgcatcg cccgtcacca
      421 cctcgaacgt ctggggtctg atcgcgacct cgccatcgtt tttcaggtcg agctgcgcgg
      481 ctccacgaag ttcatggagg agttttcggc cgccgggctt gcggaatatc tcggtctcat
      541 ccgagaggtc tttgaagagg ggcagcgcga cggcgtcttc cgccgggagc tgaacgcgaa
      601 gctcgtcgcc aaagtcatct tcggcgcttt ggacgagatg gcgaccaact ggattctctc
      661 caagcgccgc tacaagctcg cgccgatggc cgacgaggtt ctggaaatct tcttcgacgg
      721 ggtaatcgcc agatgacgga cacgcgcacg acgacgacga cgaccgccac cgaagccgcg
      781 ccggccgccg ccaccacagc acgcgccggc cacgccccgc tcccggccga tgagccgcgc
      841 acgctcgtcg aagtctgcga gcacgccctg cgcgcacgcg cgcgccccga cgcgttcaac
      901 tacaagcgcg gagacgcgtg gctcgcgatc tcctccggtg agttcgcgct ccgcgtccgc
      961 gccatcgctc tgggcttgca cgcgctcggc gtgggccgcg gcgaccgcgt ggcgattctc
     1021 tccgagagtt cgcccgagtg ggtgatggcg gacgtgggct gccagttcgc cggcgtcgtg
     1081 gacgtgccca tctacccgac gctcgccgcg ccgcagggct gctacatcat caacgactcc
     1141 ggctccgtcc tcctcttcgt ccagaacccg gcggcctacg agcgcgtccg cgaggtggcg
     1201 cgcggctgcg ccccgttgcg ccacgtcgtc atcctcgaag gcgaggcgga ggcgggcgac
     1261 ggcgacgacc cggcagtcct gacgctcgcg cagctcgaag accgcggccg cgcgctcgac
     1321 cgagagcgcc ccggcctctt cgacgagctg gccggcgccg tcggcgcgga agatttggcg
     1381 acgatcatct acacgtcggg gacgacgggc gagccgaaag gcgtgatgct cacgcactcg
     1441 aacgtcgtct cgaacctcgt agacacctcg gcgcgtctgg cctacacccc cgaagacgtg
     1501 gtgctctcgg tgctgccgct gtcgcacatc ttcgagcgcg gcgcgatgta catgtacatc
     1561 taccacggca cggccatcta cttcgccgag tcgattgagc gcatcggcga caacatgcgc
     1621 gaggtgcgcc ccaccatcgt cgtcgcggtg ccgcgtctgt ttgagaagat ttacgcgcgc
     1681 atcaaggagc gtgcggccgg gggcgggcgg gcgaagacga gcgtgctgat gtgggccgtc
     1741 tcggtggcga agcgttgggc gcgtgcgcgt tcggagcgcg agcgcgtccc cgcgctgctc
     1801 gggctgcaac acaagttggc cacgcggctc gtcttctcga agtggcagga ggggatgggc
     1861 gggcgcatcc gcgtgttttg ttcgggcggc gcggcgctgc cagaggagct gggctacatc
     1921 ttcctcgggg cggggctgcc catcgttcag ggctacgggc tgacggagac ctcgccggtc
     1981 atctgcgtca actccgtcgc cgacaatcgc atcggcaccg tgggccgagc catcgaccac
     2041 gtcgaggtac gcatcgccgc cgacggcgag atcgagacgc gcgggccgaa cgtcatgcgc
     2101 ggctactaca acaagccgga ggagaccgag aaagtcttca ccgaagacgg gtggttcaag
     2161 acgggcgaca tcggcgcgct cgaccgggac ggatacttac gcatcaccga ccgcaagaag
     2221 gagttgttca agacctcggg cgggaagtac atcgcgccgc agcccatcga gcaacgcatc
     2281 aagcagtcgc gcttcgtcaa tcaggtggtg ctcatcggca acggccgcaa gttcgccgcc
     2341 gcgctcatcg tccccgactg ggagctgctc cgctcctacg cgcagcacaa gggcatcgac
     2401 gccgcgacgc cggccgacct ctgccgccac ccgcgcgtcg tcgatctgct ccagcggcag
     2461 gtggacgccg tcaccgccga cctgtcgaac tacgagcgcg tcaagcgcat cgccctcatc
     2521 gaacacgagc tgaccgtggc cggcggcgag atgacgccca cgctcaaggt caagcgccgc
     2581 gtcgtggacg agaagtacaa ggacaccatc gaccggctct acgaggaggc ggagacgcgc
     2641 ccctatcgtt gaagtcgctg cgggcgcgcg tccgccccga aatagttgtt gccgcccgcc
     2701 cgcctgattg gtaagatggc cgccccgacg gcgaaactca cgctaacccg agccggtctc
     2761 cagctcgccg acttcaccac aggaggccac gtggcagacg cactcgacat agccatcctc
     2821 gacgcgcaag cgaagaagcg ggccgccgtc gttctggcgc ggtacgggtc ggacatcgcc
     2881 atcgccgaac tcccggctgg caaggagctg aaagcactcc tcgacttcaa agatcgcatc
     2941 agccaggtct tgctcctcag tcagaagcgg cccacgaaga aggagctgac cgagttcggc
     3001 cacgccctct tcgcctacat cgtccgcgaa aacgttgagc ggctctacaa ccgcctgccc
     3061 gtcaacacac tcatccgctt ccacatcctc gccaaccgcc ctgacctcca atcgctgccg
     3121 tgggagtacc tgcaagaccc gcagcagggg gccggcccgt ggcccaaccg ctccatcgtc
     3181 cgcgtcatcc caaccatcgg ctacacgccg cccgccccgc tccagctcgc gcagcttgcc
     3241 gccaccggca cgaagctcag aatccttttc gtctacgccg tgccgcagga tcagaatctg
     3301 gtcagttggc cgagcgtgaa gtcgagcatc gagaaggcgt tctcgctgcg catcccggcc
     3361 agccactacg agctgaaggt cattcagggg acgaccgagg agctgttcgc cgccttccat
     3421 aacaacaacg acaagtatga gatttttcag ttctccggcc acggcgacgt tgacccgcag
     3481 acgaacgagg ggcgcatcct gctcgtcaac tcgaagaaga acgaccagtc gctcccgatg
     3541 agcgccagcc agttggccag catcctccag agccgcggcg tccgcctcgc cgtcctgagc
     3601 gcctgcctta cgtcggcggg caactcggcc gacccgttca acgtcgtggc cgaggcgctg
     3661 ctcgcctccg gcatccccgc cgtcgtcgcc aaccacctgc ccgtgccgga cgcgtcggtg
     3721 gcgaccttcg tcggcgcgct ctacacgcaa ctcctcaaca cgggcgacat cgacatcgcg
     3781 gtcaacgagg gccgcctcaa gatggccatc gagctggccg tcccacagga cgcgacgctc
     3841 gaatggggca tccccaccct ctaccgctac atcaacggcg cgcaggtgtt ccagccatga
     3901 agctatcgcc attcaccctt gtctaccagc cggcgcggga ggcgcgcgtg ctgtcctccc
     3961 ggcgcgccgc gaccggggtg cagggcgtgg gccgtgtgtc cgaactggat gcgcgcccgg
     4021 aggccgtgcg cgaactcgcc cccgacctcg ccggcgcact caactccttc gacccgaacc
     4081 tcgcgcgcac gctcatcgcc agcacctacg cgcacgaatc ggccgcgccc gacgaccccg
     4141 tcgaggagaa gctgtggcgc gtcgtcaccg acatgcggcg cgccgagaag gtcgtcaccg
     4201 tcgccgtctg ggatttggat gcgagggtcg gcggcctggg gcgcctcgtc aacgcactca
     4261 acgaggcgca gcccgtcttc accttcttcg acctccaggc gccgctcccg gccggactcg
     4321 tcatcagctc cgagggcttc gccgagtggg cgcgcaaacg tctggagaag cgcgtcagca
     4381 agagtcagcg agagggcttc accgacaact tcatgttcga cgacttcaac aagttcgcgc
     4441 gcgtcgtcca caagcaggtg ggcacagact acctcgtagg catcacgcgc cacatggtgg
     4501 cgtggacgca gggcgagagc gtctactgga actacttcac ggcctccgac cggcgcgtca
     4561 tcctgacctc ggcctacaac ctgcgcgagt acgccgcact cgccggccgc cccttcgagg
     4621 ccgcggtcgc cgggctggtg gtggccgaga ttctggcggc gctcaaccgt cgcctcggct
     4681 accacgagga gaaccgcggc tgcctcttcg acttcaacca ggagcgcgac accgtcatcg
     4741 agtccatccg tcaggcgcgc atcgagccac aatgcctcga tctcatcgac gagaagtatc
     4801 gcgacgcggc cgcggccctc accgacgtgc tgcggaacta ttccccgccc cagccggacg
     4861 aggtcccgac ggagaagtct gcgaagaaga aggcggggca ggacgacgag tactggctcg
     4921 cccagctcac caagctcagc aagaagacga agtaaggaac caacggcatg aggcacaagg
     4981 agccaacggc atgacccacg cggcaatcat catccccggc atcatgggtt cggtgctgga
     5041 actcaatggc gaagtaatct ggcccggcgc ggcgtgggaa atctatctcc cctaccggaa
     5101 gatggacaag ttgctcgacc ccaacctcgt cgccaccgac gtgatccgca aggtctccat
     5161 ctccacccag tatcaaaccc tcatcgacga cctcggcaag tgcggcttcc gcgagagcga
     5221 caacccccgg acgctctacg tgtgcccgta tgactggcgc aaggacaaca ccgaggcggc
     5281 ggccaaactc gccgccctcg ccgaccaggc ggtgacgcag ggcggagcga cctccctgac
     5341 cctcatcgcc cacagcatgg gtgggctggt gagccgctac tttctggagt cgggcctcta
     5401 cgccggcaag ccggggctga aggccgtgcg ccggctgctc acgctcggca ccccacacag
     5461 gggcgcgccg ctggcgctga gcgcggcgct aggcaaggaa aagcgtctgt tcctcaacgc
     5521 cgatcaggtg ctccaggtcg ccagcgaccc gcgctacccg gccctctatc aactgctgcc
     5581 cccgcccggc gagccgtttg cctgggacga ggacaaggcc gccgagtacg gcgacgtgga
     5641 catatacgac gcgggcgtcg ccaaaaccct caagctcgtc gcgaagagtc tgaaagccgc
     5701 gaaggacttt cacgccagcc tcgacatctc caagcggccg cagtttgacg ggaagccgat
     5761 tcgctacttc ttcttctccg gcacgcggca gacgaccatc acctcggtca acgtgctgaa
     5821 ggtggacgcc acccgctacg aggtgcggaa gaccgagctg gaggacgccg gcgacgggac
     5881 ggtgccgatc tggagcgccg cgatgacggg cgtccagggg cagcccgtcg ggggcgagca
     5941 cggcaccatc taccgcaacg acacgctgcg ccgcacgatg gccgtcctgc tcggggcgaa
     6001 gggcgtgctg gccgtcgcgc ccgtgcgcgt cgaggtcgcg ctgcgcgagc gcgtcgtgca
     6061 cgacggcgac accgtgcacg ctgccctcac cttcggcgcg ggcgtcgata agctcgacgg
     6121 caaactcgcc atccacctcg tggaagtgga cgccggcggc ggggtgaaga gccggcgtct
     6181 ggtctcggag cacgacatca gctacaaggg gctgaacgcc gagaagctca acgtcatctt
     6241 cagcgcgccc gccttccccg gcatctatca agtcacctac gtcccggccg gccacaccga
     6301 cccggccggc agtgacgaac ttatcgtcca ggagagctaa aaaacacttc acgcccgacg
     6361 accgtcccgc cgcgcctcga cacaagcccg gcccggctcc gaaaagaaaa gatgcgccgg
     6421 ccgctcgct
//
LOCUS       DASVES010000064         5727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1689347, whole genome shotgun sequence.
ACCESSION   DASVES010000064 DASVES010000000
VERSION     DASVES010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689347"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..470
                     /gene="gatC"
                     /locus_tag="VF240_02365"
     CDS             147..470
                     /gene="gatC"
                     /locus_tag="VF240_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX7174116.1"
                     /translation="MPITEDDVKKIAALAHLEITDEELRQLAPQMASIVAYVEQLNEL
                     DTSGAEPAAGGLTPEGGRNDAARDDVATPSLGQALALEQAPDPSHGHFRVPKVIASSE
                     ETENF"
     gene            521..1984
                     /gene="gatA"
                     /locus_tag="VF240_02370"
     CDS             521..1984
                     /gene="gatA"
                     /locus_tag="VF240_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX7174117.1"
                     /translation="MEVIGARIAGGEGGVVRAVVEEAVGNAESLNGLLNAFLQIDREG
                     ALRRAGELDASKPSGGASPLHGVPIAIKDNICVRGLRATCGSRILAGYEPPYDATVIE
                     RLKAAGAVVVGKTNCDEFAMGSSNENSAYGPVRNPWDTERVPGGSSGGSAAAVAARIV
                     PVALGSETGGSVRQPASLCGIVGVKPTYGRVSRYGLVAFGSSLDQVSVFGVTVKDAAH
                     VIAAIAGRDARDSTTADEPVPEYAAELTGVATGARIGVPRALFGEGLDGEVRERVMAV
                     VEAYREMGAEIVDVELPHAKYSIAVYYIIATAEASSNLARYDGVRYGFRAEEAPSLQA
                     MYRRTREEGFGAEVKRRIMLGTYVLSSGYYDAYYLKAQRVRALIGQDFAGAFATCDAV
                     LTPTSPTPAFRLGEKTDDPLAMYLSDIYTCTANLAGIPGINVPCGLSSSGLPVGFQLL
                     GPHWGEPALLRLADAYERAHPLAARPRVIAGGRQGEG"
     gene            2141..3511
                     /locus_tag="VF240_02375"
     CDS             2141..3511
                     /locus_tag="VF240_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7174118.1"
                     /translation="MNRRPPAMRALALLTLLFACVSARGQEKDAPKFEVGAQFSSLSV
                     TLPENYGATNLTGFGARLSYNLNDHVALEAEGNFFANELMFSDISAGGRTVQTQFGVK
                     AGKRWERFGVFAKARPGFVSFGETLKITQGVDTLPGGRTIPSAVFGVERKTHLTLDVG
                     GVLEFYPSRRVLVRFDAGDTLIRYGERNALDYGGPGPIVLIPREWRPVGKAPAQIKHN
                     FQFSAGIAFRFMEPEGDDASTATPSSSNTNSEDAPRFEVGAQFTSLAVNLPDPVFGFL
                     VISPGDPGTDPEAGFGARFTYNLNDHIGLEAEGNFFPRQHYPGQTFGGFPVQAQFGAK
                     AGKRWERFGVFAKGRPGFISFSRVRQLVGTEQITFANPGGDPFVFTVGIFEAKRKTYF
                     SMDVGGVFEMYPSRRLMTRFDFGDTIIRYGRRHEQTFFLSQNVLEIAPETKHNFQFSA
                     GIGFRF"
     gene            complement(3705..3977)
                     /locus_tag="VF240_02380"
     CDS             complement(3705..3977)
                     /locus_tag="VF240_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174119.1"
                     /translation="MMKETRAKRTVRRRAARAGAWAVVKRALKPIPVVGTAVSVGLAG
                     HEMRKKGVLRGALHVGLDILPVVGTAKAVVEIFTGDLIPDKKPAAG"
     gene            complement(4069..4554)
                     /locus_tag="VF240_02385"
     CDS             complement(4069..4554)
                     /locus_tag="VF240_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174120.1"
                     /translation="MFQVRAAFSEQLDVKTTAERAREFFGDLKNFVELIPGVERITTE
                     GAGVMRWLIRAEIPVIGPVRHAFPVERSADAPGCIEWSPARGEKKNFLRYWASFEEQG
                     AGVTRVRLEQRVELRREHARELHPLAGLVGEGRLSAELQARLGEMMRAFLASARARLE
                     K"
     gene            complement(4665..>5727)
                     /locus_tag="VF240_02390"
     CDS             complement(4665..>5727)
                     /locus_tag="VF240_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174121.1"
                     /translation="VVARVSRNAESLHERIAQLAQQTGTHVTAVNTGFTETGDVAVGS
                     ETVVSLRRPKIIVAADEPVSVTSYGAMWWTFDRMGVDFTPMTIQAIRSARLDDFNVII
                     LPDGSPGGYFSRFGKPGADHLRAWVERGGTLILIKGAAVFGALKDVNLTSSRLVGSEE
                     DDAAAAKPDAGAATQATPTPTPAAEGAQQQGRRAQQASAQTPEQQQIASGQTEKMEGA
                     PPDLPPIASPSARPGRVPEGVPGAIFRATLDRTTPLTYGYEDVTLSVPVDSGYFFRPS
                     KEGTNALIFSADEKQPLRVAGFIWPTTEQLLRGTAYVIEEPTGRGHVVLYAEDPNYRA
                     IWRSTTRLFFNSFLFQPIF"
BASE COUNT          959 a         1959 c         1810 g          999 t
ORIGIN      
        1 ctctacatcg atgctcaaga aaaaaacgta aatcgtgaat cgtaaatcgt aaatcgattt
       61 cttcacttag actcgcgatt caccgctcaa gctgaaacac gctcaaccac gattcacgat
      121 ttaccattta cgattcacga gcctttatgc ccatcaccga agacgacgtt aagaagatcg
      181 ccgcgctcgc gcatctggag atcacggacg aggagctgcg gcagctcgcg ccgcagatgg
      241 cctcgattgt cgcttacgtc gagcagttga acgagcttga cacgagcggc gccgagccgg
      301 ccgccggcgg actcacgccc gagggcgggc gcaacgacgc cgcgcgcgac gacgtggcga
      361 cgccgtcgct cggacaggcc ctcgcgctcg aacaagcgcc cgacccttcg cacggtcact
      421 tccgcgtgcc gaaggtgatc gcgagttccg aggagacgga aaatttctga gcgctcgcgc
      481 cgttcggtgt ttgattcaca tgccttcatc agaaaacaca gtggaagtta ttggggcgcg
      541 catcgcgggc ggcgaggggg gcgttgtgcg cgccgtcgtt gaagaggccg tcggcaacgc
      601 cgagagtctg aacggcctgc ttaacgcttt cctccagatc gaccgcgagg gcgcgctccg
      661 gcgggccggt gagcttgacg cgtcgaagcc gtcgggcggc gcatccccgc tccacggcgt
      721 gcccatcgcg attaaggaca acatctgcgt gcgcggtctc cgggccacct gcggctcgcg
      781 catcctcgcc ggatacgagc cgccttacga cgcgaccgtg atcgagcgtc tgaaagcggc
      841 gggcgctgtc gtcgtcggca agacgaactg cgacgagttc gcgatgggtt cgtcgaacga
      901 gaactcggcc tacggccccg tgcgtaaccc gtgggacacg gagcgcgtgc cgggcggctc
      961 ctcgggcggc tcggcggcgg ccgtcgcggc gcgcatcgtc cccgtcgcac tcggctcgga
     1021 gacgggaggc tcggtgcgcc agcccgcctc tctgtgcggc atcgtcggcg tcaagcccac
     1081 ctacggccgc gtctctcgct acggcctcgt cgccttcggc tcgtcgctcg atcaggtgag
     1141 cgtcttcggc gtcaccgtca aagacgccgc acacgtcatc gcggccatcg ccggccgcga
     1201 cgccagagat tcgacgaccg ccgacgagcc ggtgcccgaa tacgccgcgg agttgacggg
     1261 cgttgccacg ggcgcgcgca tcggcgtgcc gcgcgcgctc ttcggcgagg gtctggatgg
     1321 ggaggtgcgc gagcgcgtca tggcggtggt cgaggcttac cgcgagatgg gggcggagat
     1381 cgttgacgtg gagttgccgc acgcgaagta ttccatcgcc gtctactaca tcatcgccac
     1441 ggccgaggcg tcgtccaatc tcgcgcgcta cgacggcgtg cgctacggct tccgcgccga
     1501 ggaggccccg agccttcagg cgatgtaccg gcgcacgcgc gaggagggct ttggcgccga
     1561 ggtcaagcgc cgcatcatgc tcggcacata cgtcctctcc tccggctact acgacgccta
     1621 ctacctcaag gcccagcgcg tgcgcgcgct catcgggcag gacttcgcgg gagcgttcgc
     1681 cacctgcgac gcggtgctga cgccgacctc gccgactccg gccttccgcc tcggcgagaa
     1741 gaccgacgac ccgctcgcca tgtatctgtc ggacatctac acctgcacgg ccaacctcgc
     1801 cggcatcccc ggcataaacg tcccctgcgg cctctcctct tcggggctgc ccgtcggctt
     1861 ccaactcctc ggcccgcact ggggcgagcc ggcgctcctg cgtctcgccg acgcctacga
     1921 gcgcgcgcac ccgctcgccg cgcggccccg cgtgatcgcc ggcgggagac aaggcgaggg
     1981 atgagggatg agggatgaat tgaagactag tcctgtctaa ttcatccctc atccctcatc
     2041 ctttcagttg gccgcacact tccgcgaaaa tattttcccc gccccgcgtg ggttccgcct
     2101 cccgtcaggc gtcatacctt caagaggaga gacgaaaggc atgaaccgac gcccgcccgc
     2161 catgcgcgcg ctcgcgctcc tgacactgct gttcgcgtgt gtctccgcgc ggggtcagga
     2221 aaaagacgcg ccgaagttcg aggtcggcgc gcaattctcg tcgctctccg tcaccttgcc
     2281 cgagaactat ggcgccacca acctgacggg cttcggcgct cgcttgagct acaacctcaa
     2341 cgaccacgtc gcgctcgaag ccgagggcaa cttcttcgcc aacgagttga tgttctcgga
     2401 catctcggcg ggcgggcgga cggtgcagac gcagttcggc gtcaaggcgg gaaagcgttg
     2461 ggagcggttc ggcgtctttg cgaaggcgcg ccccggtttc gtcagcttcg gcgagactct
     2521 gaagatcacc caaggggtgg acacgctccc cggcgggcgg acgattccct ccgccgtctt
     2581 cggcgtcgag cgcaagaccc acctcacgct ggacgtgggc ggcgtgctgg agttctaccc
     2641 ctcgcgccgc gtcctcgtgc gcttcgacgc cggcgacacg ttgatccgct acggcgagcg
     2701 caacgcgctc gactacggcg gccccggccc catcgtcctc atcccgcggg agtggcggcc
     2761 cgtcggcaag gcgcccgcac agatcaagca caacttccag tttagcgccg gcatcgcctt
     2821 ccgcttcatg gagccggaag gagacgacgc ttcgacagcg acgccttcat cttcaaacac
     2881 aaacagcgag gacgcgccgc gcttcgaggt cggcgcgcag ttcacttcgc tcgcggtcaa
     2941 tctgccggac cccgtcttcg ggttcctggt tatctcgccg ggtgacccag ggacagaccc
     3001 ggaggcgggg ttcggcgcca gattcacata caacctgaac gaccacatcg gcctggaggc
     3061 cgagggcaac ttcttcccgc gccagcacta tccggggcag actttcggcg gcttccccgt
     3121 gcaggcgcag ttcggcgcga aggcgggaaa acgctgggag cgcttcggcg tcttcgccaa
     3181 gggccgcccc ggcttcatca gcttcagccg cgtccggcag ctcgtcggca cagagcagat
     3241 aacgttcgcc aatcccggcg gcgacccgtt cgtcttcacc gtcggcatct tcgaggccaa
     3301 gcggaagacg tatttctcga tggacgtggg cggcgtcttc gagatgtacc cctcgcgccg
     3361 cctgatgacg cgcttcgact tcggcgacac catcatccgc tacggccgcc gccacgagca
     3421 gaccttcttc ctctcgcaaa acgtcctcga aatcgctccc gagacgaagc acaatttcca
     3481 gttcagcgcc ggcatcggat ttcgttttta atcacggcca tcagacacac ggcgcgcaag
     3541 ccacaacgag ccgcgaagcg gcggcacatc ggtaacgcga accagatgtg ccgccgcttc
     3601 gcggctccgt tgattacttg ctggattaac cacgggctga cgcccgtggc tattggctgc
     3661 cgccgcttcg cggctcgttg tcgcctccgg cgactcgtcg aattttaacc agccgccggc
     3721 ttcttgtcgg ggatgaggtc gcccgtgaaa atctcgacga cggccttggc cgtgccgacc
     3781 acgggcagga tgtccagccc gacgtgcaat gcgccgcgca gcacgccctt cttccgcatc
     3841 tcgtgtccgg ccagccctac agacacggcc gtccccacga cgggtatcgg cttcagcgcc
     3901 cgcttcacca ccgcccacgc gcccgcacgc gccgccctgc gcctgaccgt tctcttcgct
     3961 cgcgtttctt tcatcatttc agccccatcg cagttcaaag ttcaaagttc aaagttcaaa
     4021 gtcaacagcc gccgaacgcg ctttgaactt tgaactttga actttgaact atttttccag
     4081 acgcgcgcgg gcgctcgcga gaaaggcgcg catcatctcg ccgaggcgcg cctgaagctc
     4141 ggcgctcaga cgcccctcgc cgacgagtcc ggcgagcggg tgcagctcgc gcgcatgctc
     4201 ccgacgcagc tcgacgcgct gctcaagacg cacgcgcgtc accccggccc cctgctcctc
     4261 gaacgaggcc cagtagcgca ggaaattctt cttctcgccg cgcgccggcg accactcgat
     4321 gcagcccggc gcgtcggccg aacgctccac ggggaaggcg tggcgcacgg ggccgattac
     4381 gggaatctcc gcccggatca gccagcgcat cacgcccgcg ccctcggtcg tgatgcgctc
     4441 cacacccgga atcagctcca cgaagttttt caaatccccg aaaaactcgc gcgcccgctc
     4501 cgcggtcgtc ttcacgtcca actgctcgct gaaagctgcc ctcacttgga acactggaac
     4561 ctccgtgata agtgatgagt gataagtgat aagaaagaag aaagccgtct tcctctcttt
     4621 cttatcactt atcactcatc acttatcact catcacttat caccttaaaa aattggctga
     4681 aacagaaacg agttgaagaa cagacgcgtg gtgctgcgcc agatggcgcg gtagttcggg
     4741 tcctcggcgt agaggacgac gtggccgcgg cctgtcggct cctcgatgac gtaggccgtc
     4801 ccgcgcagga gttgctcggt cgtcggccag atgaagccgg cgacgcgcaa gggctgtttc
     4861 tcgtcggcgc tgaagatgag cgcgttggtg ccctctttcg aggggcggaa gaagtagccg
     4921 gaatcgacgg ggacggagag cgtcacgtct tcgtagccgt aggtgagcgg agtggtgcgg
     4981 tcgagcgtgg cgcggaagat ggcgccgggc acgccctcgg gcacgcgccc cggtcgggcc
     5041 gagggcgagg cgatgggcgg caggtcgggc ggcgcgccct ccatcttctc ggtctgcccg
     5101 gaggcgatct gctgctgctc gggcgtctgc gccgacgcct gctgagcgcg acgaccttgc
     5161 tgctgcgcgc cctccgcggc gggcgtcggc gtgggcgtcg cctgcgttgc cgccccagcg
     5221 tcgggcttcg ccgccgcggc gtcgtcctct tcgctgccga cgagtcgcga tgatgtgagg
     5281 ttcacgtctt tgagcgcgcc gaagacggcc gcgcccttga tgaggatgag cgtgccgccg
     5341 cgttcgaccc aggcgcgcag gtgatcggct ccgggcttgc cgaagcgcga gaagtagccg
     5401 ccgggcgagc cgtctgggag gatgatgacg ttgaagtcgt cgaggcgcgc ggagcggatg
     5461 gcctgaatcg tcatcggcgt gaagtctacg cccatgcggt cgaacgtcca ccacatcgca
     5521 ccgtagctcg tcacgctgac aggttcgtcg gctgcgacga tgatcttggg ccggcgcaag
     5581 ctgaccaccg tctcagagcc gacggccacg tcgcccgtct cggtgaagcc ggtgttgacg
     5641 gccgtgacgt gtgtgcccgt ctgttgcgcg agctgcgcga tgcgttcgtg caaactctcc
     5701 gcgttgcgcg agacgcgcgc gacgacg
//
LOCUS       DASVES010000065         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1297742, whole genome shotgun sequence.
ACCESSION   DASVES010000065 DASVES010000000
VERSION     DASVES010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297742"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VF240_02395"
     CDS             complement(<1..1454)
                     /locus_tag="VF240_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174122.1"
                     /translation="MLKKSFFATAVVAVASALLFCAAQTTSPAPAKRRAQVRLLTREV
                     SLGRIHPGELKETRTLSPDRRRIAFVAKVAGGEAVFVDGVAGKTYPSVENDPLSEAGP
                     GSPITFSPDGRRVAYVAYVGPAGTGARGPRLVVVDGKEGAPFDYVWSGSPHFSADGRR
                     FAYTAERGGKWHAVVDGVESKAFDRVSQPIFSKDGARASFSAERAGRRLIVADGVESD
                     ADDPAGPTQLQGATSGSAPREVEQGGKRFVVWEGGKGGPYEEVRDVGRSAAGDRVIFS
                     AKRDGREFAVLDGKEGKPYDDVGSIHISPDGQRVMYVAQLDKKSFVVLDGAEGAKYDS
                     IEVSYPSSFSPDGKRYAYKAARETSPDSREYFVVADGKEGKSYDHVDGIAFDADGRLL
                     YLAYNFSDRKTFLVREGEEVASYDNTPGYGDFKFSPDGKRMFFRLNKLGEGKEALVLD
                     GRAFAYDEIKNAEFGPGGRRFIFKARRAQGWVMVL"
     gene            1660..>3444
                     /locus_tag="VF240_02400"
     CDS             1660..>3444
                     /locus_tag="VF240_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX7174123.1"
                     /translation="MRTTLTRSLLLLTFLLSAIQLDAHAQLNPYAPDTAEPGSVEAIA
                     RFTTEPRFLSPWVAYVPESARVPSPTKFLGHVVGAPGELSNTSKVYGYMRELDRASER
                     VRVETIGKSEEGRDILLVAIADEEGLRELDRLKAATAALADPRKTTPEQAERIIETAR
                     PIYYFNAGLHSTETGSPEMVMELAHRLAVSEQPMIQNIRRNLIVLINPVSEPDGRDKT
                     VDWFYRYLKGKTDFENLPPTSPPYWGFYVYHDNNRDTHQKALQLTRAVHRMFYDYHPT
                     VVHDLHESIPLLQTWNGTGPWNTNLDSILLSEFFEMSFHEVGALTGMGMPGVWTWGFG
                     EGWGHHYLDSVAVNHNSMGRGYETFGNTTAETVERVLRPNEERYVDRPVTTREWYRPF
                     PPDRRFKWSLRNNTNYMETGCLSILDYTAKHSKEMLRNFYRKHYNSWQKGVKGDPFAY
                     VIAAEQGDRRRVAEMLNVLMGHRIEVSRATAPFKVAEGEYPAGAYVVRLDQPYRNYAV
                     DLLEPQKFPTDTPFQPYDDVSWALPAHYGVEAKRIADAKIKDVPLEPVTAELRPAGRI
                     AGDGPFFLLKDTGQESLLAARQRLAGYRI"
BASE COUNT          603 a         1212 c         1060 g          569 t
ORIGIN      
        1 agaaccatca cccacccctg agcgcgccgc gccttgaaga tgaagcggcg tccgccgggg
       61 ccgaactcgg cgtttttgat ttcgtcgtaa gcgaacgcgc ggccgtccaa gaccagcgcc
      121 tctttgccct cgcccagttt gttcaggcga aagaacatgc gcttgccgtc gggactgaat
      181 ttgaagtcgc cgtagccggg ggtgttgtcg taggaggcga cctcttcgcc ttcacggacg
      241 agaaaggtct tgcggtcgct gaagttgtag gcgagataga ggaggcgtcc gtcggcgtcg
      301 aaggcgatgc catcaacgtg gtcgtaagac ttgccctctt tgccgtccgc gacgacgaag
      361 tactcgcgtg agtcggggct tgtctcccgc gcggccttgt aggcgtagcg tttgccgtcg
      421 gggctgaagg acgaaggata gcttacttcg atgctgtcat acttcgcgcc ctccgcgccg
      481 tctaagacga cgaacgactt tttgtcgagc tgcgccacat acatcacgcg ctggccgtcg
      541 gggctgatgt gaatgctgcc cacgtcgtcg taaggcttgc cctctttgcc atccaatacc
      601 gcgaactccc ggccgtcgcg ctttgcggag aagatcactc ggtcgcccgc ggcgctccgg
      661 ccgacatcgc gcacttcctc atacggcccg cccttaccgc cctcccacac gacgaaacgc
      721 ttgccgccct gctcgacctc gcgcggcgcg ctcccgctcg tcgcgccctg caattgcgtc
      781 ggccccgccg ggtcgtcggc gtcggactcg acgccgtccg ccacgatgag ccgccgcccc
      841 gcgcgctcgg cgctgaagga cgctcgcgcg ccgtccttgc tgaagatggg ctgcgagacg
      901 cggtcgaacg ccttgctctc gacgccgtca accacggcgt gccacttgcc gccgcgctcg
      961 gcggtgtagg cgaagcgccg gccgtcggcg ctgaaatgcg gcgaccctga ccagacgtag
     1021 tcgaacggcg cgccctcctt gccgtccacg acgaccaggc gaggcccgcg cgcacccgtg
     1081 ccggcaggcc cgacgtaggc gacgtaagcg acgcgccggc cgtcggggct gaaggtgatg
     1141 gggctgcccg gcccggcctc gctcagcggg tcgttctcca ccgacgggta agtcttgccc
     1201 gcgaccccgt ccacgaagac ggcctcgccg cccgcgacct tcgccacgaa ggcgatgcgc
     1261 cggcgatccg gcgagagcgt gcgcgtctcc ttcaactcgc cggggtggat gcgtccgagt
     1321 gagacctcgc gcgtcaagag gcgcacctgt gcgcgccgct tcgccggggc gggcgaggtc
     1381 gtttgcgccg cgcagaagag caacgccgac gcgaccgcca caacggccgt cgcgaaaaaa
     1441 gacttcttca gcatctctcg aacctccgcc gccggcgcac gcctttcgcg ccggcggcct
     1501 agactctaca gcgccgcgcg cgtttctgtg aagccctggc ccccgcggcg cacacgccat
     1561 ttctgacgaa cgcggaaagt gaagctgtgt cataatccgg cgcgtcaaat ccgtcaaagc
     1621 tctatccacc acgacgaagg caggtcttcg caagtctcaa tgagaaccac actgacgcgc
     1681 tcgctcctcc tcctgacgtt tctactctcc gcgatccaac tcgacgcgca cgcgcaacta
     1741 aacccttacg cgccggacac ggccgagccg ggttcggtcg aggcgattgc gcgcttcacg
     1801 accgagccgc gctttctcag cccgtgggtc gcctacgtgc cggagtcggc gcgcgtgccg
     1861 tcgccgacga agttcctcgg acacgtcgtc ggcgcgccgg gtgagttgtc gaacacgtcg
     1921 aaggtctacg gctacatgcg tgaattagac cgcgcctcgg agcgcgtgcg cgtcgagacc
     1981 atcggcaaga gcgaggaggg ccgagacatc ctgctcgtgg cgattgccga cgaggagggc
     2041 ctgcgcgaac tcgaccggct caaggcggcg acggccgcgc tcgccgaccc gcgcaagacc
     2101 acccccgaac aggccgagcg catcatcgag acggcgcgtc ccatctacta cttcaacgct
     2161 gggttgcatt cgaccgagac cggcagcccc gagatggtca tggaactcgc ccaccgtctc
     2221 gccgtctccg agcagccgat gattcaaaac atccggcgca acctgatcgt cttaatcaac
     2281 cctgtctccg aacccgacgg ccgcgacaag accgtcgatt ggttctacag gtatctgaag
     2341 ggcaagaccg acttcgagaa cctgccgcca acgtcgccgc cctactgggg cttctacgtc
     2401 taccacgaca acaaccgcga cacgcaccag aaggccctgc aactaacgcg cgccgtccac
     2461 cgcatgttct acgactatca cccgacggtc gtccacgacc tccacgagtc catcccgctc
     2521 ttgcagacgt ggaacgggac ggggccgtgg aacaccaacc tcgactccat tctcctcagc
     2581 gagtttttcg agatgtcctt ccacgaggtc ggcgcgctca ccgggatggg aatgccgggc
     2641 gtgtggacgt ggggcttcgg cgaaggctgg gggcatcatt atctcgactc ggtcgccgtc
     2701 aatcacaaca gcatgggccg cggctacgag accttcggca acaccacggc cgagacggtc
     2761 gagcgcgtgc tccggcccaa cgaggagcgt tacgttgacc ggcccgtcac cacccgcgag
     2821 tggtacaggc cgttcccgcc cgaccggcgc ttcaagtggt cgctgcggaa caacacgaac
     2881 tacatggaga ccggctgcct ctccatcctc gactacacgg ccaagcactc gaaggagatg
     2941 cttcggaact tctatcgcaa gcactacaac tcctggcaga agggcgtgaa gggcgacccc
     3001 ttcgcctacg tgattgcggc cgaacagggc gaccgccgcc gcgtcgccga gatgctcaac
     3061 gtgctcatgg gccaccgcat cgaggtgagc cgcgcgacgg cgccgttcaa ggtcgccgag
     3121 ggcgagtacc cggccggcgc ctatgtcgtg cgtctcgacc agccctaccg caactacgcc
     3181 gttgaccttt tggagccgca gaagttcccg accgacacgc ccttccagcc ttacgacgac
     3241 gtgtcgtggg cgctgcccgc tcactacggc gtcgaggcga aaagaatagc cgacgcgaag
     3301 attaaagacg tgccgcttga gccggtcacc gcggagcttc ggcccgcggg ccggatcgcg
     3361 ggcgatggcc ccttctttct tctcaaggac acggggcagg agtctctgct cgcggcccgg
     3421 cagcgtctcg ccggctaccg gata
//
LOCUS       DASVES010000066         4965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_909387, whole genome shotgun sequence.
ACCESSION   DASVES010000066 DASVES010000000
VERSION     DASVES010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909387"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1211
                     /gene="thiL"
                     /locus_tag="VF240_02405"
     CDS             42..1211
                     /gene="thiL"
                     /locus_tag="VF240_02405"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX7174124.1"
                     /translation="MKAEGRKRHPDSAHPLSSPKHRSEFDFIEQLRHRAFRRKENGVN
                     NSHSSLITHHSSLLQGIGDDAAVLHTSDASFDTVVTADLLVEDIDFRLTTTTPRLLGH
                     KALAVSLSDIAAMGARPRWALLSIGAPRRLWDGPFLEEFYEGFFALAEEHGVALVGGD
                     VSRTGAGGRSSSRAGLLVIDSIVLGEATRGRSVLRSGARPGDRIYVTGALGGAAAGLR
                     LLEGGARAPQDAETRPSKTLPKSDTPGARRRRLMLRQLRPEPRVLWGRTLGEKKLASA
                     MIDLSDGLSSDLAHLCRESGVGARIDAARVPVDPSVARALKLSPDGALKLALSGGEDF
                     ELLFTSGARDASRLPKRVGGVAVSRIGVVTDEPGRVVLVGGGRERVLEAEGYRHF"
     gene            1464..1949
                     /locus_tag="VF240_02410"
     CDS             1464..1949
                     /locus_tag="VF240_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7174125.1"
                     /translation="MQHFAVRIAVAFLTFIIGTAASAMWGLFNMSRATVTQGQFVYVG
                     TKSCPSTRPAADIPPPPPPPSFNGPTINGGILNGKAISKPAPAYPAIAKAARASGTVV
                     VRIIVDESGRVSEAEAISGHPLLQQAAVAAVRQWRFSPTLLSGQPVKVAGTVSVNFNL
                     Q"
     gene            1973..3379
                     /gene="cysS"
                     /locus_tag="VF240_02415"
     CDS             1973..3379
                     /gene="cysS"
                     /locus_tag="VF240_02415"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7174126.1"
                     /translation="MLRFHNTLSGRLEEFRPLRDGEVRMYFCGPTVWELAHIGNLRSN
                     VFVDVLRRYLKFKGYQLTHVMNITDVDDRIISQALSKGLSIDDYTAPFIEAFWQDFEA
                     VGCERPEIAPRATRHIAEMEEITRRLVERGHAYASDGSVYYRISSFPDYGKLSKINFA
                     GNIAGASERVDTDKYDKEDARDFVLWKAQKTADEPAWDTSFGRGRPGWHVECSAMSMK
                     YLGETFDIHCGGVDLIFPHHENEIAQSEGATGKEFVKYWLHGEHLKIDSETMSKSKGN
                     YYTLRDLLKMGFTTAAVRYALLSVPYRKQINFTIDGLRGAEKTVAGLQDFHARLSETR
                     AEPGANPEMRAAAARALEEFESGLDDDLNTSVALAAVHNLTREVNTALACAGLRADDK
                     RDLLALVARFDSVLGVFGRPAREMLDSEIQSLIDDRQEARRRRDFSRADEIRDQLASR
                     GIVLEDTRDGVRWKRRSS"
     gene            3573..4931
                     /locus_tag="VF240_02420"
     CDS             3573..4931
                     /locus_tag="VF240_02420"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX7174127.1"
                     /translation="MSTIAPAKRPEIKTSTLPGPRGQEIVEADAQFVTPSYPRPSFKL
                     VADTASGVWVTDVDGNVFLDCNAGVAVCSTGHCHPEVVRAIQDQAAQLIHMCGTDYYY
                     RHMPELAKKLDEIVPVPSPTRTHFANSGTEAVETALKLAMHATGREKFIAFFNSFHGR
                     TLGSLSLTSSRVAQRRGFKRQALDVVHVPYPNIFRDPFGGVPTDAETVSRMCLDWIEQ
                     RLFKTTTPPDEVAGIVVEAVQGEGGYVPAPKAFLHGLRRICDEHGIMLIVDEVQSGFG
                     RTGKMFACEHYDLKPDIITLAKGIASGIPMGACVARADLMDWKPGAHASTFGGNPVAI
                     AAALKTIEILERELVANSAEVGAYMQDGLRRLMQKHECIGDVRGLGMMIGVEFVESRD
                     NLKAAPELRDRVETECFNRGLIILGAGFNTIRFSPPLILSKENVDVALEIFDEAITAS
                     CS"
BASE COUNT          881 a         1769 c         1489 g          826 t
ORIGIN      
        1 cgctctcgac tcgatgcgcc gcaatttgtg aaggatgaag gatgaaagct gaaggaagga
       61 agcgacaccc ggattccgct catcctcttt cgtcacccaa gcaccgcagc gagttcgact
      121 tcatcgagca attaagacat cgtgccttcc gaagaaaaga gaacggagtc aacaactctc
      181 actcatcact catcactcat cactcatcac tccttcaagg tatcggcgac gacgccgccg
      241 tcctccacac ctccgacgca agcttcgaca ccgtcgtcac ggctgacctg ctggtcgaag
      301 acatagactt ccgactcacg accacgacgc cgcgtctcct cggccacaag gcgctcgccg
      361 tctcgctctc cgacatcgcc gcgatgggcg cgcgcccgcg ctgggccttg ctctccatcg
      421 gcgcgccccg gcgtctgtgg gacggcccct tccttgaaga attttacgaa ggcttcttcg
      481 cactggccga ggagcacggc gtcgcgctcg tcggcggcga cgtttcgcgc acgggcgctg
      541 gcggccgctc gtcgtcgcgc gcgggcctac tcgtcatcga ctcgattgtc ctcggcgagg
      601 ccacgcgcgg gcgctccgtc ctgcgctcgg gcgcgcggcc gggcgatagg atttacgtga
      661 cgggcgcgct cggcggggcc gccgcgggtc tccgcctgct cgaaggcggg gcgcgcgcgc
      721 cgcaagatgc ggagacgaga ccgagtaaaa ctttgccgaa gtcagacacg ccgggagcgc
      781 ggcgacggcg actcatgctg cgtcaactgc gtcccgagcc gcgcgtcctc tgggggcgga
      841 cgctcggcga gaaaaaactt gcgtcggcaa tgatcgactt aagcgacggc ctctcgtccg
      901 acctcgcgca cctctgccgc gagagcggcg tgggcgcgcg catcgacgcc gcgcgcgtcc
      961 ccgttgatcc gtcagtcgcg cgcgccttga aactctcccc cgacggcgcg ctcaagcttg
     1021 ccctctccgg cggcgaagat ttcgagcttc tcttcacctc cggcgcgcgc gacgcctccc
     1081 ggctgccaaa gcgcgtcggc ggggtcgcgg tctcacgcat cggcgtcgtc acggacgaac
     1141 ccggccgggt cgtactcgtg ggtgggggac gtgagcgcgt ccttgaggcg gaaggctaca
     1201 gacatttctg aacggcgtcc cactcagggc tgtgtggagt cgcgcgagag attcgggccg
     1261 cgtggggcga cgggaaaact cggaactgcc gggcaatggt aaaaaaactg taaataattt
     1321 gactcgtcac cccggccttg cctagactct caccaagaca attgttttaa gccgccacag
     1381 tgcggcgcag ttgaatgttt ccgccccgtc cggccgtcgc cgtctcccgg ccagcggggc
     1441 cgtggctttt gaagggagct gtcatgcaac acttcgcagt ccgaatcgcc gtcgctttcc
     1501 tgaccttcat catcggcacc gccgccagcg cgatgtgggg tctcttcaac atgtcgcggg
     1561 ccaccgtgac gcagggccaa ttcgtctacg tggggacgaa gtcgtgcccg tcaacgaggc
     1621 cggccgccga catcccgccg cccccgccgc cgcccagctt caacgggccg acgattaacg
     1681 gcggcatcct caacggcaag gccatcagca agcccgcgcc cgcgtacccg gccatcgcga
     1741 aggccgcgcg cgcgtcgggc acggtggtcg tgcgtatcat cgttgacgag tcgggccggg
     1801 tgagcgaggc cgaagccata agcggccatc cactgctgca acaagcggcc gtcgcggccg
     1861 tgcgccagtg gcgcttctcg ccgacgctgc tctcgggcca acccgtcaag gttgcgggga
     1921 cagtctccgt caacttcaac ctgcaataaa tatcggcgcg gcgcgctcct ctatgctcag
     1981 gttccacaac actctctccg gccgcctcga agagtttcgc cccttgcgcg acggcgaggt
     2041 gcgaatgtac ttctgcggcc ccaccgtgtg ggagctggcg cacatcggaa acctgcgctc
     2101 gaacgtcttc gtcgatgtcc tgcgccgcta tctgaagttc aagggctacc agctcaccca
     2161 cgtcatgaac atcaccgacg tggacgaccg catcatcagt caggcgctct cgaagggcct
     2221 ctccatcgac gactacaccg cgcccttcat cgaagccttc tggcaggact tcgaggccgt
     2281 cggctgcgag cgacccgaga tcgcgccgcg cgccactcgc cacatcgccg agatggagga
     2341 gatcacccgc cgtctcgtcg agcgcggcca cgcctacgcg tccgacggct cggtctacta
     2401 ccgcatcagc tccttccccg actacggcaa gctctcgaag atcaacttcg ccggcaacat
     2461 cgccggggcg agcgagcgcg tggacaccga caagtacgac aaagaagacg cgcgcgactt
     2521 cgtgttgtgg aaggcgcaga agacggcgga cgagccggcg tgggacacct cgttcgggcg
     2581 gggacggccc ggttggcacg tcgagtgctc ggcgatgtcg atgaagtatc tcggcgagac
     2641 cttcgacatc cactgcggcg gcgtcgatct catcttcccg catcacgaga acgagatcgc
     2701 gcagagcgaa ggggcgacgg gcaaggagtt cgtgaaatac tggctccacg gcgagcacct
     2761 gaagatcgac agcgagacga tgtcgaagtc gaagggcaac tactacacgc tgcgcgatct
     2821 cttgaagatg ggttttacga cggcggccgt ccgctacgcg ctcttgagcg tgccctaccg
     2881 caagcagatc aacttcacca tcgacggcct gcgcggcgcc gagaagaccg tcgccggcct
     2941 gcaagacttc cacgcccggc tctcggagac gcgcgccgaa cccggcgcca accccgagat
     3001 gcgcgccgcg gccgcccgcg cgctcgaaga gttcgagtcg ggcttagacg acgacctcaa
     3061 cacctcggtc gcgctcgccg ccgtccacaa cctgacgcgc gaagtaaaca ccgccctcgc
     3121 ctgcgccggc ttacgcgcgg acgacaagcg cgacctcctc gcactcgtcg cgcgcttcga
     3181 ctcggtgctg ggcgtcttcg gccgcccggc gcgcgagatg ctcgacagcg aaatccagtc
     3241 cctcatcgac gaccggcagg aggcccgccg ccgccgcgac ttctcgcgcg ccgacgaaat
     3301 ccgcgaccag ctcgcctcgc gcggcatcgt cctcgaagac acccgcgacg gcgtgcgctg
     3361 gaagcgccgc agctcgtagt gttaagattc cacagtttcc ggccgcgccg cgccgcagtt
     3421 attgatatga gcgccaatct ctcgtccacc aacatccgcc cgttagacgc ccgcgccaac
     3481 ctctttgcgg actagcaagc cgccgtcccc ctcgctcctg cgtcctcgat ttatcattcc
     3541 cttcccgcgt tcttagacaa aggagaacat ttatgtcaac cattgctccc gcaaagagac
     3601 ccgagatcaa gacttcaact ctgcccggcc cgcgcggtca ggagatcgtc gaggctgacg
     3661 cgcagttcgt cacgccttcc tacccgcgtc cgtccttcaa gctcgtcgcc gacacggcct
     3721 ccggcgtctg ggtcacagac gttgacggca acgtctttct cgactgcaat gccggcgtcg
     3781 ccgtctgctc caccggccac tgccaccccg aggtcgtccg cgccattcag gatcaggccg
     3841 cccagctcat ccacatgtgc ggcaccgatt actactatcg acacatgccc gagctggcga
     3901 agaaactcga cgagatcgtg cccgttccct cgccgacgcg gacacacttc gccaacagcg
     3961 gcaccgaggc cgtcgagacc gcgctcaagc tcgccatgca cgccaccgga cgcgagaagt
     4021 tcatcgcctt cttcaacagc ttccacggcc gcacgctcgg aagtctgtcg ctcacctctt
     4081 cgcgcgtcgc acagcgtcgc ggctttaagc ggcaggcgct cgacgttgtc cacgtcccct
     4141 acccgaacat cttccgcgac ccctttggcg gcgtgcccac cgacgccgag accgtcagcc
     4201 ggatgtgtct cgactggatc gagcaacgcc tgttcaagac cacgacgccg cccgacgagg
     4261 tcgccggcat cgtcgtcgaa gccgtgcagg gcgagggcgg ttacgtcccc gcgccgaaag
     4321 ctttcttaca cggcctgcgc cgcatctgcg acgagcacgg catcatgctc atcgtggacg
     4381 aggtgcagtc cggcttcggc cgcacgggca agatgtttgc gtgcgagcac tacgacctca
     4441 agcccgacat catcacgctg gcgaagggca tcgcctccgg catcccgatg ggcgcctgcg
     4501 tggcgcgcgc cgatctgatg gactggaagc ccggcgcgca tgcctccacc ttcggcggca
     4561 accccgtggc catcgccgcc gcgctcaaga ccatcgagat tctggagcgc gagctggtcg
     4621 ccaactccgc cgaggtcggc gcttacatgc aggacggcct ccgacgactc atgcagaagc
     4681 acgagtgcat cggcgacgtg cgcggtctgg gcatgatgat cggcgtcgag ttcgtcgagt
     4741 cgcgcgacaa tttgaaggcc gcgcccgagc tgcgcgaccg cgtcgagaca gagtgcttca
     4801 accgcggcct catcatcctc ggcgccggct tcaacaccat ccgcttctcg ccgccgctca
     4861 tcctctcgaa ggagaacgtt gacgtggcgc tggagatatt cgacgaggcc atcaccgcct
     4921 cctgctcctg acacgcacga cagtaccgcg agcggaagcc tcgcg
//
LOCUS       DASVES010000067         9561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_908259, whole genome shotgun sequence.
ACCESSION   DASVES010000067 DASVES010000000
VERSION     DASVES010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908259"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..892)
                     /locus_tag="VF240_02425"
     CDS             complement(83..892)
                     /locus_tag="VF240_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7174128.1"
                     /translation="MNQKLLVTLDNGVKRITFNRPERRNAVDLEMFAAFAEAIADSAR
                     DESRVVVIAGAGDSFCAGLDLSTIAAGDPASLDVAATVRELINPPILRMRALSKPIIA
                     AVHGAAVGIGFSYVLASDVRVATETATFSQSFVRVGLMPDGGSTFFLPRLVGRTRAFE
                     LMATGATLKAEEALRLGLVNRVVAANELEAVVGELAARMAAAPQPSLARIKGALSRAE
                     QEELAAALEYEADGQADCFRSADFREGVAAFMQKREPVFGKHKTSAAKGDE"
     gene            complement(1117..1731)
                     /locus_tag="VF240_02430"
     CDS             complement(1117..1731)
                     /locus_tag="VF240_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174129.1"
                     /translation="MGLVEMQKALARLYTNADLRERFFNDPATVARELGFGDEEARRL
                     AAVSPARIDSFARSLHNKRLGQVAKLLPLSKRALGGPFSEAFRVFADTYVPAGTKKHL
                     GDALAFAGHLEKLLRARKGQHPRWALDLLRYEKARIKAADPRRRVVVCVFRHDISQLV
                     RSLARKEDAAVFTRPCVAVWARLRRGTTIRYAVYMVPELRRKKN"
     gene            complement(1722..2600)
                     /locus_tag="VF240_02435"
     CDS             complement(1722..2600)
                     /locus_tag="VF240_02435"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX7174130.1"
                     /translation="MAVAAVAAAVSEGDAFAARLPALGVGLAFREPLRGDVFLRRAEI
                     DFLEITADHYLDAAPEKQRELDLLREHFHLVPHALDLSLGSAEGVDRVYLRKLARLVR
                     RLDPPWWSEHVAFTRAGGVEIGHLAPTEFSREAARVFARNVKEVRAHIDAPLILENIT
                     YTLTSPATTWGEAEFLNEVLELTNCGLLLDVTNLHINSVNHGYDFRDFLERLPLDRVV
                     QLHFVGGHRHGDELIDSHSERTPEEVWDIMDGVLARAPVRGAILERDENLPPIEELLA
                     EVGRAREMGRRHGRWA"
     gene            complement(2566..3603)
                     /locus_tag="VF240_02440"
     CDS             complement(2566..3603)
                     /locus_tag="VF240_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HEX7174131.1"
                     /translation="MEWLWYNPIADMRGPTFLLFYACASAATIAVCAMLSRQLDWTGS
                     MQPPPLPSTPDPYEIAYLRGGENEVARTAVFALMRSGHLHVIQRGKKSLIERTGPPAP
                     TAALGHFERRAYGWFDHPQESAAVFRQGGLAEKLRRHCADYEQKLRGEKLLTPPEASD
                     RARLVRLLGLAVIAGLGAYKLAIALSRGRTNVIFLILLGAVALVVLFKVCRSPRLSRR
                     GRAHLERLRTAFDRLKGGGQTLTETATMARESADERGPYATPGGLDPALLTLGVFGVA
                     ALAGADHDNYTRAFNRSTATADSSGGSASSCGSASSWSDSSSSGGDSGGGDSGGSSCG
                     GGCGGCGGGGE"
     gene            3911..5824
                     /locus_tag="VF240_02445"
     CDS             3911..5824
                     /locus_tag="VF240_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174132.1"
                     /translation="MKVVFPSPRTTRLAPAHAYVTVPARRLALLLLALLLLPAAPAAL
                     RAQQQQTPTTPEQKPAPDAPAPAPVGEVIDEDDVLRIDSNLVTIPALVTETTGRPVPN
                     LKVEDFELRVDGQLKQIGEMSRADTPVVLAVLFDNSSSLSTAREFEKQAAVTFFRRIV
                     RPIDSAAVYSVSTVPVLARPLTNDVSALVRTVEQFAEPEGATALFDAVSQAADYLRTR
                     DARRVIVVVSDGNDTISESSFDHAIQRALKYDCQIYIVGTGDIENQNFTDTIARRRLQ
                     VFAAQTGGALYVPRTVEDLDHAFEQISDDISQQYVLSYHPSDDPPDGRFRQIELRVKT
                     RPTLRVRAREGYYAGAGQAQIASAAQTPSRVRQRTVGAPKTGAARGGANKAQGSALKD
                     QPAQVASLDPLYASPAAGSMRESAAAARDIKIKTAPTSSARSARNGPRENADTSEPER
                     PAPSASTAEAQPVQTTYEPSPSRTPGTSLFGSSASITPSAPLSTATTAADPQPQPPKT
                     RSTTLEVARGDSVNSSAPKPAPPTNPEPAPSKSTPPAPVSGGVLNGKALRLPAPAYPS
                     AARNVRAGGKVTVEVEIDEEGRVTRAQAVSGHSMLQSAAVNAARQARFSPTLLSGAPV
                     RITGQIVYNFIQP"
     gene            complement(5880..6560)
                     /gene="aat"
                     /locus_tag="VF240_02450"
     CDS             complement(5880..6560)
                     /gene="aat"
                     /locus_tag="VF240_02450"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX7174133.1"
                     /translation="MVIRFPDPRDAGDDGLLAVGGDLRPETLLAAYRSGIFPWPIEGW
                     PLPWFSPQERAVLVFDELHVPRRLARERRSTRLRFTIDQNFRGVIEACARARRPEGPG
                     TWITGAMIRGYCELHRRGHAHSVEAWEDDELVGGLYGVDADGAFSGESMFYRRANASK
                     LALLFLVEHLRGRGLEWVDVQQLTPHMRALGAREVARETFLDMLAATRGRGLKLFPTL
                     KQGMRDEG"
     gene            6646..6960
                     /locus_tag="VF240_02455"
     CDS             6646..6960
                     /locus_tag="VF240_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX7174134.1"
                     /translation="MANYDPEFDEAVEAESREKLEKPPLFKVLLHNDDFTTMEFVVHV
                     LQTVFGHDEAAAVRIMLNVHTQRVGVAGVYTYEVAETKADAAARLARAHEYPLLCTVE
                     EE"
     gene            7017..7490
                     /locus_tag="VF240_02460"
     CDS             7017..7490
                     /locus_tag="VF240_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174135.1"
                     /translation="MRDGFFKHPRVTPFVECPNCARLVEFGAEHCPACREVIEKGYAL
                     LSVGVVAFNTQAVSLANTIKTGDAFAVIILLASAYVFFLDVSDAELSFQSLWMTTLAA
                     SLLALSAVALWFFRYGRFRMGDADFDRSKLDMRWSLMLWLALLFAQAVAFATLYL"
     gene            7588..>9561
                     /locus_tag="VF240_02465"
     CDS             7588..>9561
                     /locus_tag="VF240_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7174136.1"
                     /translation="MMITKELEETLNAALSEALKRRHEYVTLEHLLYALAHERTASQV
                     IRHCGANIEEVVSDLETFFKDQRSAPRSGRGRVPEHTLAFQRVIEYALLQAEGSGQKE
                     VNGGNVLAALFQAEQSQAVYFLKKQGVTRLDVLNYLAHGISKLDATGAGSAVPAGDDE
                     LAAQTESAQAQKDPLDAFAVNLIERAASGKIDPLIGRAAELQRTIQVLCRRRKNNPIF
                     VGDPGVGKTAIAEGLALKIQQGDVPDVIKSAEVYALDMGALLAGTKYRGEFEQRLKAV
                     IVALKKKENVILFIDEIHTIVGAGAVSGGSMDASNIIKPVLASGEIRCIGSTTYPEYK
                     AAFERDKALARRFQRIEIGEPSVEDTYEILQGLKSYYEEHHHVRYTDDALRSATELSA
                     KHIHDRFLPDKAIDVIDEAGAAVKLLPADERPETIDVNEIETVVARMAKIPPRTVAGS
                     ERERLSKLEQELKAVIYGQDNAVSQVVNAIKLSRSGLGHQDHPIGSFLFSGPTGVGKT
                     ELAKQLAHVLGVEFLRFDMSEYMEPHTVSRLIGAPPGYVGFDQGGLLTDAVNRTPYAV
                     LVLDEIEKAHPNLFNILLQVMDHATLTDNNGKRADFHNVILIMTTNAGAREMSDAKIG
                     FQHGEGGGAGRGKGRGVIERTFSPEFRNRLDAWI"
BASE COUNT         1640 a         3326 c         3027 g         1568 t
ORIGIN      
        1 tcggcctcgt ctaattcggc gttgaatgta tcgtgactgt gggcagggac aagccctgcc
       61 cctacagttt tgcgacgtac tttcactcgt cgcctttcgc ggcgcttgtc ttgtgcttgc
      121 cgaacactgg ctcgcgcttc tgcatgaagg ccgcgacgcc ttcgcggaag tcggcggagc
      181 ggaaacagtc ggcctggccg tcggcttcgt attcgagggc ggcggcgagt tcctcctgct
      241 cggcgcggct gagtgcgccc ttgatgcggg cgagcgaggg ctgcggcgcg gcggccatcc
      301 gcgcggccaa ctcgccgacc accgcctcca actcattcgc ggcgacgacc cggttgacga
      361 ggccgagccg caacgcttcc tccgctttga gggtcgcgcc ggtcgccatc agctcgaagg
      421 cgcgggtgcg gccgacgaga cgcggcaaaa agaaagtgct gccgccgtcg ggcatgaggc
      481 cgacgcggac gaagctctgg ctgaaggtgg ccgtctccgt ggccacgcgc acgtcggagg
      541 cgaggacata gctgaagccg atgccgacgg ccgctccgtg gacggcggcg atgatgggct
      601 tgctcaacgc gcgcatcctg agaatgggcg ggttgatcag ctcgcgcacg gtggcggcca
      661 cgtcgagact cgccgggtcg ccggcggcga tggtggagag gtcgaggccg gcgcagaagg
      721 agtcgcccgc gcccgcgatg acgacgacgc ggctctcgtc gcgcgccgag tcggctatcg
      781 cctcggcgaa ggccgcgaac atttcgagat cgacggcgtt gcgccgctcg ggacggttga
      841 aggtgatgcg cttgacgccg ttgtcgagcg tgacaagtag cttttgattc atgttcatca
      901 tctctagggg tagtaacaac tcacctgccc gtgtgccacc aggaggagtt aacatggatg
      961 ggcaggatac aagaagtgat gagtgatgag tgataagtga tgagaagaag ccggattgtt
     1021 tttcttatca cttatcactc atcactcatc acttcttctc gtcctgacca tcctgtccat
     1081 ccatgttaat tctttctgtc ttctccgccc tcgcagttaa ttcttttttc gccgcagctc
     1141 cggcaccatg tagacggcgt agcgaatggt tgtgccccgg cgcaggcgcg cccagacggc
     1201 gacgcagggg cgtgtgaaga cggcggcgtc ctccttgcgt gcgaggctgc ggacgagctg
     1261 gctgatgtcg tgtcgaaaga cgcagacgac gacgcggcgg cgcgggtcgg cggccttgat
     1321 gcgggccttt tcgtagcgca gcaggtcgag cgcccagcgc gggtgctgac ctttgcgcgc
     1381 gcgcagaagt ttttcaaggt gcccggcgaa ggccagcgcg tcgccgaggt gcttcttcgt
     1441 cccggcgggc acgtaggtgt cggcgaagac gcggaaggct tcggagaagg ggccgccgag
     1501 cgcgcgcttc gagagcggga ggagcttggc gacctgtccg agccgcttgt tatggaggga
     1561 gcgggcgaag gagtcgatgc gggcgggcga cactgccgcg agccgccgcg cctcctcgtc
     1621 gccgaagccg agctcgcggg cgaccgtggc ggggtcgttg aaaaagcgtt cgcgcaagtc
     1681 cgcgttggtg tagagccgcg ccagcgcctt ctgcatctcg actaagccca tcggccgtga
     1741 cgcctcccca tctcgcgcgc gcggcccacc tccgccaaca gttcttcgat gggcgggagg
     1801 ttctcatcac gttcgaggat cgccccgcgg acgggcgcgc gcgccagaac gccgtccatt
     1861 atatcccaca cctcctcggg cgtgcgctcc gagtggctgt cgattaattc gtcgccgtgt
     1921 cggtggccgc cgacgaagtg gagctgcacg acgcggtcga gcggcaggcg ttcgagaaag
     1981 tcgcggaagt cgtagccgtg gttgacggag ttgatgtgga ggttagtcac gtcgagcagc
     2041 aagccgcagt tcgtcagctc caacacttcg ttcagaaact cggcctcgcc ccacgtcgtc
     2101 gcgggcgagg tgagcgtgta ggtgatgttt tcgagaatga gcggggcgtc gatgtgcgcg
     2161 cgcacctctt tgacgttgcg tgcgaagacg cgtgcggcct cgcgcgagaa ctcggtgggc
     2221 gcaaggtggc cgatctcgac gccgcccgcg cgcgtgaagg cgacgtgctc gctccaccac
     2281 ggcgggtcga ggcggcgaac gaggcgcgcg agcttgcgta ggtaaacacg gtcaacgccc
     2341 tcggcgctcc cgagcgagag gtcgagcgcg tgcgggacga ggtggaagtg ctcgcgcagg
     2401 aggtcgagtt cgcgctgctt ttcgggcgcg gcgtcgagat agtggtcggc ggtgatttcg
     2461 aggaagtcga tctcggcgcg gcgcaggaac acgtcgccgc gcagcggctc gcggaaagcc
     2521 agtccgacgc cgagcgcggg cagacgcgcc gcgaaagcgt ccccctcact caccgccgcc
     2581 gccacagccg ccacagccac ctccacaact acttccgccg ctgtctccgc cgccgctgtc
     2641 gccaccgctc gaagagctgt ccgaccaact actggccgac ccgcacgagg aggcggaacc
     2701 accggacgaa tcggccgtgg ccgtcgagcg attgaaggcg cgtgtgtagt tgtcatggtc
     2761 ggcgccggcg agcgccgcca cgccgaacac gccgagcgtg agcagcgccg ggtcgaggcc
     2821 gcccggtgtc gcatacgggc cgcgctcgtc ggccgactcg cgcgccatcg tagcggtctc
     2881 cgtcagagtt tggccgccgc ccttcagacg gtcgaaggcc gttcttaaac gttcaaggtg
     2941 cgcccgcccg cgtcggctca ggcgtgggga acggcacacc ttgaagagga cgacgagcgc
     3001 gacggccccg aggagaatca ggaagatgac gttcgtgcgt ccgcgtgaga gcgcgatggc
     3061 gagcttgtaa gcgcccagac cagcgatgac cgcgaggccc agcagccgca cgagccgcgc
     3121 gcggtcgctg gcctcgggcg gcgtcaaaag cttttcgccc cgaagcttct gctcatagtc
     3181 ggcgcagtgt cttctcaact tctcggccag tccgccctgc ctgaacactg ccgccgactc
     3241 ttgcgggtgg tcgaaccatc cgtaggcgcg gcgctcgaag tggcccagcg cggccgtcgg
     3301 cgcgggcggg cccgttcttt caatcagcga cttcttaccg cgttgtatga cgtgcaggtg
     3361 gccgcttcgc atgagcgcga agacggccgt gcgggccact tcgttttcgc cgccgcgcag
     3421 gtaggcgatc tcgtaggggt caggcgtcga gggcagtggc ggcggctgca tggagcccgt
     3481 ccagtcgagc tggcgcgaga gcatcgcgca gacggcgatg gtcgcggccg aagcgcacgc
     3541 gtagaagaga aggaacgtgg ggccgcgcat gtcggcgatt gggttgtacc agagccattc
     3601 catagtgttc acctcccatc aagaaatttg actctatacg cctcgtcgtc agcgccggtt
     3661 ccgccatttt atgtgccggc acacgtgtta agaaaggctt gtttgcgccc ttccgtgaaa
     3721 ctagtgctcc aatgagaata tcagaattgt gtcggctgcg gctcaggttt tccgtcgccg
     3781 ggtatgggcg cgcggcccgg cccaaaccgc tcgccggaca gaacttgtgc gcttcggcga
     3841 tttgtggcag agtgtgttcc ccggcacgcc tttcccggcg gcctttgacc ttaccccgtg
     3901 tgaatctcta atgaaagttg ttttcccctc ccctcgcacg acccgactcg cccccgcgca
     3961 cgcatacgtt accgtccccg cgaggcggct cgcgctcctt ctcctcgcgc tcctcctcct
     4021 cccagccgcg cccgccgccc tgcgcgcgca gcagcagcag acgcccacga cgcccgaaca
     4081 gaaacccgcg cccgacgcgc ccgccccggc gcccgtcggc gaagtcatcg acgaggacga
     4141 cgtgctgcga atagactcca acctcgttac catccccgcc ctcgtcacgg agactacggg
     4201 gcggcccgtc cccaatctca aagtcgaaga cttcgagctg cgcgtggacg ggcagttgaa
     4261 gcagatcggc gagatgagcc gcgccgacac tccggtcgtc ctggccgtcc ttttcgacaa
     4321 cagctccagc ctttcgacgg cccgcgagtt tgagaagcag gccgccgtca ccttcttccg
     4381 acgcatcgtc cgccccatcg actcggccgc cgtctactcc gtctcgaccg ttccggtgct
     4441 cgcccgtccg ctgacgaacg acgtgagcgc gctcgtccgc accgtcgagc agttcgccga
     4501 accggagggc gccaccgcgc ttttcgacgc cgtctcgcag gcggccgact atctccgcac
     4561 gcgcgatgcg cgccgcgtca tcgtcgtcgt ctccgacggc aacgacacca tcagcgagtc
     4621 gagtttcgac cacgccatcc agcgcgcgct caagtacgac tgccagattt acattgtcgg
     4681 caccggcgac atcgagaatc agaacttcac cgacaccatc gcccgccgcc gccttcaggt
     4741 cttcgccgcg cagacgggcg gcgcgctcta cgtcccgcgc acggtcgaag acctcgacca
     4801 cgccttcgag cagatttccg acgacatctc gcagcagtac gtcctcagct atcatccttc
     4861 ggacgatccc ccggacggac gcttccgcca gatcgaactg cgcgtgaaga cccgccccac
     4921 tctgcgcgtc cgcgcgcgcg aaggttacta cgccggcgcg gggcaagcgc aaatcgcatc
     4981 ggccgctcag acgccgtcgc gcgtccgcca acggacggtg ggcgctccga agacgggcgc
     5041 ggcgcgcggc ggcgcgaaca aggctcaggg atcggcgctc aaagatcagc ccgcgcaggt
     5101 cgccagcctc gacccgctct acgcgtcgcc ggccgccggc tcgatgcgtg agtcggccgc
     5161 ggcggcgcgt gatatcaaga tcaagaccgc gccgacctct tcagcgaggt cggcgcgcaa
     5221 cggcccgcgg gagaatgcgg acacctcgga gcctgagcgc cccgccccgt ccgcctccac
     5281 ggccgaagcg cagcccgtgc aaaccaccta cgagccttcg ccttccagaa cgcccggcac
     5341 ttccctcttc ggctcgtccg catcaatcac tccctcggct cccctctcaa ccgcaacgac
     5401 ggccgccgac ccacagccgc agccgccgaa gactcgcagc acaacacttg aagtcgcccg
     5461 cggcgacagc gttaacagca gtgcccctaa gccggcgcct ccgacaaacc cggaacctgc
     5521 cccgtcgaag tccacaccgc ccgcgcccgt ctccggcggc gtgctcaacg gcaaggcgct
     5581 ccgcctgccg gcgcccgcct acccgtccgc cgcgagaaac gtgcgcgcgg gcggcaaggt
     5641 gacggtcgag gtggagatcg acgaggaggg ccgcgtcacg cgcgcgcagg ccgtctcggg
     5701 ccacagcatg ctgcaaagcg cggccgtcaa cgccgcgcgg caggcgcgct tctcgcccac
     5761 gctgctctcc ggcgcgcccg tcagaatcac cggccagatc gtctacaact tcatccagcc
     5821 gtaggggaag ggatgaagga tgagggatga gggatgaata gttgttctca tctcatccct
     5881 catccttcat ccctcatccc ttgcttgagc gtcggaaaga gtttaagccc acgcccgcgc
     5941 gtcgcggcga gcatatcgag gaaggtctcg cgggccacct cacgcgcgcc gagtgcgcgc
     6001 atgtgcggcg tcaactgttg cacgtctacc cattcaagcc cgcgccccct caggtgctcg
     6061 acgagaaaga gcagcgcgag cttcgacgca ttcgcccgcc ggtagaacat gctctcgccc
     6121 gagaaggcgc cgtcggcgtc cacgccgtac aacccgccca ccagctcatc atcctcccac
     6181 gcctcgacgc tgtgcgcgtg cccgcgccgg tgcagctcgc agtagccgcg aatcatcgcg
     6241 cccgtgatcc acgtccccgg cccttcaggt cgccgggcgc gcgcgcatgc ttcgattacg
     6301 ccgcgaaagt tttggtcgat ggtgaatcgc aggcgcgtgc tccggcgctc gcgcgcgagg
     6361 cgacgcggga cgtgcagctc gtcgaacaca agcaccgcgc gctcctgcgg cgagaaccac
     6421 ggcagcggcc acccttcaat cggccacggg aagatgccgc tgcgataggc cgcgagcaga
     6481 gtttcggggc gcaggtctcc gccgacggcc aacagcccgt cgtcgcccgc gtctctcggg
     6541 tcggggaatc gaatcaccat cacgccgacc agcttatggg cgagcgcagt cgccgggcaa
     6601 gccctccctt gcccgccccg cgtcccggcc cttacactta gcgggatggc gaactacgac
     6661 ccggagttcg atgaggccgt cgaggccgag agccgcgaga agctggagaa gcctccgctc
     6721 ttcaaggtgc tgctgcacaa cgacgacttc acgacgatgg agttcgtcgt ccacgtcctc
     6781 cagaccgtct tcggccacga cgaggcggcg gccgtccgta tcatgctcaa cgtccacacg
     6841 cagcgtgtgg gcgtggccgg cgtctacacc tacgaggtgg ccgagacgaa ggccgacgcg
     6901 gcggcccgcc tcgcgcgcgc ccacgagtac ccgctgctgt gtacggtgga agaggagtag
     6961 acgcgcccga taggaggcgg ccggctcggt ttattccgcg cggccggcga cgaaagatga
     7021 gagacggctt cttcaaacac ccgcgggtca cgccgttcgt cgagtgcccg aactgcgccc
     7081 ggctcgtcga gttcggcgcg gagcactgcc ccgcctgccg cgaggtgatc gaaaagggct
     7141 acgcgctttt gagcgtcggc gtggtcgcct tcaacacgca ggccgtgagt ctcgccaaca
     7201 ccatcaagac cggcgacgcc ttcgccgtca tcatccttct ggcgtccgcc tacgtcttct
     7261 tcctcgacgt gtccgacgcc gagttgtctt ttcagtcgct ctggatgacg acgctcgccg
     7321 cttcgttgct ggcgctctcc gccgtcgcgc tctggttctt ccgctacggc cgcttccgca
     7381 tgggcgacgc ggacttcgac cgctcgaagc tcgacatgcg ctggtcgctc atgctgtggc
     7441 tcgcgctgct gttcgcgcag gccgtcgcct tcgccacgct gtatctgtga acaaggagtg
     7501 agcagtgagc agtgagcagt aagcagtaag aacgcgcaac tcgtcttgac tgctcactgc
     7561 tcactgctca ctgctcactg aaaagggatg atgatcacta aagaactcga agagaccctg
     7621 aacgcggcgt tgtcggaagc gctcaagcga cgccacgagt acgtgacgct cgaacacctc
     7681 ctctacgcgc tcgcgcacga gcgcacggcc agtcaggtca tccgccactg cggcgcgaac
     7741 atcgaggaag tggtctccga tctggagacc ttcttcaaag accagcgcag cgccccgcgc
     7801 tccgggcgtg gccgcgtccc cgagcacacg ctcgccttcc agcgcgtcat cgagtacgcg
     7861 ctcttgcagg ccgaagggtc gggtcagaag gaggtcaacg gcggcaacgt cctcgccgcg
     7921 ctctttcagg ccgagcagtc gcaggccgtc tactttctca agaagcaggg cgtcacgcgt
     7981 ctcgacgttt tgaactacct cgcgcacggc atctcgaagc tcgacgcgac gggcgcgggc
     8041 agcgccgtcc ccgccggcga cgacgaactc gccgcgcaga ccgagtcggc gcaggcgcag
     8101 aaagacccgc tcgacgcctt cgccgtcaac ctcatcgagc gcgcagccag tggcaagatc
     8161 gacccgctca tcggccgcgc cgcggaactc caacgcacca ttcaggtgct ctgccgccgg
     8221 cgcaaaaaca accccatctt cgtcggcgac cccggcgtcg gcaagacggc catcgccgag
     8281 ggcctcgcgc tcaagattca acagggcgac gtgcccgacg ttatcaagag cgccgaggtc
     8341 tacgccctcg acatgggcgc gctgctcgcc gggacgaagt accgcggcga gttcgagcag
     8401 cgcctcaagg ccgtcatcgt cgcgctcaag aagaaggaaa acgtcatcct cttcatcgac
     8461 gagattcaca ccatcgtcgg ggccggcgcc gtctccggcg gctcgatgga cgcctcgaac
     8521 atcatcaagc cggtgctggc ctccggcgag atacgctgca tcggctcgac cacttatccc
     8581 gagtacaagg ccgccttcga gcgcgacaag gcgctcgccc gccgcttcca gcgcatcgag
     8641 atcggcgagc cttccgtcga agacacttac gagattttgc agggccttaa gagttactac
     8701 gaagagcacc atcatgtgcg ctacaccgac gacgctctgc gctccgccac cgagctgtca
     8761 gccaaacaca tccacgaccg cttcctcccc gacaaggcca tcgacgtgat cgacgaagcc
     8821 ggcgccgccg tcaaactact cccggccgac gagcgccccg agaccatcga cgttaacgag
     8881 atcgagacgg tcgtcgcccg catggcgaag attccgccgc gcaccgtcgc cggctccgag
     8941 cgcgagcgcc tctcaaagct tgagcaggaa ttgaaggccg tcatctacgg tcaggacaat
     9001 gccgtctctc aggtcgtcaa cgccatcaag ctctcgcgct ccggcctcgg tcatcaggat
     9061 caccccatcg gctccttcct cttctcgggg ccgacgggcg tcggcaagac cgagcttgcc
     9121 aagcagctcg cccacgtcct cggcgtcgag ttcctacgct tcgacatgag cgagtacatg
     9181 gagccgcaca cggtctcgcg tctcatcggc gccccgcccg gctacgtcgg cttcgatcag
     9241 ggcggactcc tcaccgacgc cgtcaaccgc acgccctacg ccgtcctcgt cctcgacgag
     9301 atcgagaagg cgcaccccaa cctcttcaac atcctcttgc aggtcatgga tcacgcgacc
     9361 ctgaccgaca acaacggcaa gcgcgctgac ttccacaacg tcatcctgat aatgaccact
     9421 aacgccggcg cccgcgagat gagcgacgcg aagatcggct tccagcacgg cgagggcggc
     9481 ggtgcgggtc gcggcaaggg ccgcggcgtc atcgagcgca ccttcagccc cgagttccgc
     9541 aaccgcctcg acgcctggat c
//
LOCUS       DASVES010000068         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_114768, whole genome shotgun sequence.
ACCESSION   DASVES010000068 DASVES010000000
VERSION     DASVES010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114768"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VF240_02470"
     CDS             complement(<1..846)
                     /locus_tag="VF240_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7174137.1"
                     /translation="MMKTSTRRRAHVFAAAAALVSLTALPAASRAQSDIASSSGVFVE
                     PAGRPRPSKKHGGTAPSRRAAAKRKTGGGAASAGAQAVAANAQGLALSKQGRHADSVE
                     HFKKAIRLKPKFQDAHYGLGVAYFETGRFSEAVASLKEATQLDPKDAAAWDYLGSSYT
                     KMSRPAEAVAAYQQAALFDADDAEVHHSLADAYMSAGQPEQAVESLKRAVTLDPNLTE
                     AHSELGDYYIKQKRFQEAVDAYKNLARLTPQDSEAYSNLGDSYRGLGNFKEAVAAYQQ
                     AVRLKP"
     gene            983..2107
                     /locus_tag="VF240_02475"
     CDS             983..2107
                     /locus_tag="VF240_02475"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX7174138.1"
                     /translation="MAEVKRVGVLTGGGDAPGLNPAIKGLVYRASEFDIEVSGIYDGW
                     RGLLDPAPESPALDRERVRRWDRDGGTNLGSSRTNPFKSPTPEGERIDRSDEVAANVR
                     RLGLDAVVVMGGEDTLGVAARLSEKGVSVVGVPKTIDKDLAGTDYTLGFDTALRNVTE
                     VIERSRTPAGSHGWVQIIEVMGRHAGHLALWSGVAGQADLILIPEHPFRYEKVFRHLR
                     ERMGDPTSAAAARDPERPRYSVVVVSEGAYAEDGELVTVDDRHDAFGHARLGGIGEVL
                     ARRVMAETDFDARAAALGHPQRGGPPSAIDRVMGMMFGARAAEAVAAGEFGKMVSARG
                     IAPACELSLVEITTVLGKLNTVDVERHYDTERYHLKGIGM"
BASE COUNT          339 a          789 c          810 g          384 t
ORIGIN      
        1 gggcttcagg cgcacggcct gctggtaggc cgcgacggcc tccttgaagt tgccgagacc
       61 gcggtatgag tcgcccagat tcgagtaggc ttccgaatcc tgtggggtga ggcgcgcgag
      121 attcttgtag gcgtcaaccg cctcctgaaa gcgcttctgc ttgatgtagt agtcgccgag
      181 ttcggagtgg gcttcggtga ggttggggtc gagtgtgacg gcgcgcttta acgactcgac
      241 ggcctgctcg ggctggcccg cgctcatgta ggcgtcggcg agggagtgat ggacttcggc
      301 gtcgtcggcg tcgaagagcg cggcctgctg gtaggcggcg acggcctcgg cggggcggct
      361 catcttggtg taagacgagc cgaggtaatc ccaggcggcg gcgtccttcg ggtcgagctg
      421 cgtcgcctcc ttgagagagg cgacggcttc actaaagcgg cccgtctcga aataggcgac
      481 gccgaggccg tagtgtgcgt cctgaaactt cggcttgagc cggatcgcct ttttgaagtg
      541 ctcgaccgag tcggcgtggc ggccctgctt actcagagcc agtccctgcg cgttcgccgc
      601 cacggcctgt gcgccggccg acgcggcgcc gccgccggtc ttccgcttgg ccgcggcgcg
      661 tctggacggg gccgtccccc cgtgtttctt cgaggggcgt gggcggccgg cgggttcgac
      721 gaagacgccc gaggagctgg cgatgtcgct ctgcgcccgc gacgccgcgg ggagggccgt
      781 gagcgagacg agtgcggcgg cggcggcgaa gacgtgtgcg cggcgtcgcg tcgaagtttt
      841 catcatcgtg gaactcccgt ggtgatgcgc cgttccggcg aggtggtcgg ggcgtgtttt
      901 gctgtccggt ctgaggtgag tttatactga cgcccacgca atcaccaaaa aactttttcc
      961 cgaaggagtg actcggcacc gcatggctga agtgaagcgc gtcggcgtgc tgacgggcgg
     1021 cggagatgcg ccgggcctca accccgcgat caagggcctc gtctaccgcg cctctgagtt
     1081 cgacatcgaa gtctccggca tctacgacgg ctggcgcggc ctgctcgacc ccgctcccga
     1141 gtcgcccgcg ctcgaccgcg agcgcgtccg ccgctgggat cgcgacggcg gcaccaacct
     1201 cggctcctcg cgcaccaacc ccttcaagtc gcccacgccc gagggcgagc gcatcgaccg
     1261 ctccgacgaa gtcgccgcaa acgtccgccg ccttggcctc gacgccgtcg tcgtgatggg
     1321 cggcgaggac accctcggcg tggcggcgcg tctgtcggag aagggcgtct cggtagtcgg
     1381 cgtgccgaag acgattgaca aagacctcgc cggcaccgac tacacgctcg gcttcgacac
     1441 ggcgctgcgt aatgtcaccg aggtcatcga gcgttcacgc acgcccgccg gctcgcacgg
     1501 ctgggtgcaa atcatcgagg tgatgggccg ccacgccgga cacctcgcgc tctggtcggg
     1561 cgtggcgggg caggccgatt tgattctcat ccccgaacac ccgttccgct acgagaaggt
     1621 cttccggcac ctgcgcgagc gcatgggcga cccgacctcg gcggcggccg cgcgcgaccc
     1681 tgagcgcccg cgctactccg tcgtcgtcgt gtccgagggc gcctacgccg aggacggcga
     1741 actcgtcacc gtggacgacc gccacgacgc cttcggccac gcgcgtctcg gcggcatcgg
     1801 cgaagtgctc gcccgccgcg tcatggccga gaccgacttc gacgcgcgcg cggccgccct
     1861 cggacacccg caacgcggcg gcccgccctc ggccatcgac cgcgtcatgg gcatgatgtt
     1921 cggcgcgcgc gcggccgagg ccgtcgccgc cggcgagttc ggcaagatgg tctcggcgcg
     1981 cggcatcgcc ccggcctgcg agctttccct cgtcgagatc acgaccgtcc tcggcaagct
     2041 caacaccgtg gacgtggagc gccactacga cacggagcgt taccacctga agggcatcgg
     2101 gatgtgattt agagggggcg cgcggcgcag ggcgcgggcg ctatacgccc tccctcacgg
     2161 ccgggctacg gccttcaatg ccactcgctt gacctgctct cgcgcagcgt gttaggttcg
     2221 cctctgttag caatacaccc gaacaattcg tgaactcgaa cggcaggaga cgagtgtgac
     2281 cgcgccctcc ctcaagcaga cgcccctgaa cgccgcgcac cg
//
LOCUS       DASVES010000069         9668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1409927, whole genome shotgun sequence.
ACCESSION   DASVES010000069 DASVES010000000
VERSION     DASVES010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409927"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VF240_02480"
     CDS             <1..233
                     /locus_tag="VF240_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7174139.1"
                     /translation="RQTALAAATQPGPRVNINKASRAELERLPGVGPATASRIVEHRE
                     QYGPFRRAEHLLLVRGFSDSRFRELRPLIAVD"
     gene            315..1643
                     /locus_tag="VF240_02485"
     CDS             315..1643
                     /locus_tag="VF240_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX7174140.1"
                     /translation="MNELSELRRAAREIFDHALAESDAGRAVRRAVRLDGARLNIFDD
                     EFDLDRPARVYSVAAGKAARPMAAALDEALGARLIRGVVSAPLAGATLSGRWEVYAGA
                     HPLPNAASLDAARAAFNLLREANRPEALVLFLVSGGGSAMIESPRAADVTLEDLREAN
                     RALVACGAPIAEVNAVRRALSAVKGGGLSLRAPAAAQVTLIVSDVNAGCESDVASGPT
                     FPQPDDARAASHVVGKYGLGSQLPASVLRLIEHPPADLAAQAAPRALRRHHVLLDNAR
                     AVQSAVESARARGLMAEAMPELVEQHVSEGSAALVARLSELQRRAGGRVVCLVSGGEF
                     ACPVRGDGIGGRNAETALRCALEFEKMAGGPSHVVALCAGTDGIDGNSPAAGALADST
                     TLARARSRGLDPHRHLEDSDAHTFFHALDDALLTGPTGTNVRDLRILLTE"
     gene            complement(1712..2083)
                     /locus_tag="VF240_02490"
     CDS             complement(1712..2083)
                     /locus_tag="VF240_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX7174141.1"
                     /translation="MNQGDASDTSPLFDRRPWGSFTVLDEGAGYKVKRIEVLPGKRLS
                     YQRHARRAEHWMVVAGTGRVTLDGREITVHTDETIDVPVGAAHRIENPGADTLIFIEI
                     QRGDYLGEDDITRLEDDFGRG"
     gene            complement(2580..3614)
                     /locus_tag="VF240_02495"
     CDS             complement(2580..3614)
                     /locus_tag="VF240_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174142.1"
                     /translation="MKLRILLPMLFVLTFFTWAFAQTTSNCIKRSCSATNRPGGAGCD
                     IVFEYKDCSNQNHTARPSCTNGIQGQSSCQCTCTAAPSNGWAVSYSLPDDSNVSEQKA
                     CLGCPTPTTSQSCYEFGWHWNYAHNYCSETPAPCPEQQYMCAEGWQYWDEWQCGCYGT
                     PPSPIVIDVAGNGFNLTDAAGGVTFDISSSGHPMRISWTAANSDDAWLALDRDGNGTI
                     DGGRELFGNVAPQPTPPAGQERQGFLALAEFDKPESGGNADGVINRRDAIFDSLRLWQ
                     DTNHNGVSEANELHPLPDLGLRTIDLDYKESRRTDQHGNQFKYRAKVRDARGHQLGRW
                     AWDVFLVSGQ"
     gene            complement(3666..4454)
                     /locus_tag="VF240_02500"
     CDS             complement(3666..4454)
                     /locus_tag="VF240_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174143.1"
                     /translation="MKKASPMLLLLLAALGLTLAFALSKKSRTSLAAAAGAQEVKTHL
                     AQKKDFLSESGQRVLEVVEASYQTTPDSFDSSSVRAKNLSGKNITALGLVWTVTYEDK
                     TNCQIEQLIDYRLHRDIVADRKVRAFAPDEEKVIPRLTKDTLEEGRGIESVSVEFAFA
                     ELEEAGAVGSDPSGLYKQLRDKRAGAEIYKRWVESGYDDTPAQFEAVVGKLSGDELPA
                     DKALENDKAVLGALVYKQWMLGVLKDKGAAALQKQLRQSRQPSR"
     gene            complement(4621..7359)
                     /locus_tag="VF240_02505"
     CDS             complement(4621..7359)
                     /locus_tag="VF240_02505"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX7174144.1"
                     /translation="MLVPHQPSFNLHPLALLAASFAAGILLARLISPPLAACFAGGII
                     LSTLAVVACLRERAGHATVLVVAAFAACGAVFASAEGVATPRVPRVRQLLEEGRIGGG
                     DPVELTGVLERAPEVAPDGLALRLRAERWGFKGEEREVSGAVELFAPARSEKVWAEYD
                     ALELRRGARLRVLVGLDRDEGYRNPGVSPFTEYLERRDADARGTIKSPLVVERLDDEP
                     VFLPLLWLERRRAWLLARFTDNFSADTAGVLAASVLGNRLHLARATGEKFREGGTFHV
                     LVISGLHITFVGGVAWWLARRLTRRRLWQWAASAVCVWAYAVAVGAEASVLRAALMFT
                     VAALAPAIGRPAGTLNALGGAALALLAARPRALFDPSFQLTFLSVLSIVVLAWPALTR
                     LHEVGAWRPRSTTPYPPACPALLRRLAEALYWSERRWRREMRRSTYSYRLFKTPLAAR
                     LERWRVQAALRYVFTAVLVSACVQTGMLPALVLYFHRLTPAALVLNVFVGALMAVLSF
                     AALAAVLVAQVSAKAAAPLVWLAEWANRLMVDSAGPFAAAGLASVRLPEYAGAAAFVY
                     ALYYLPLAVLALSLAHWSPLALRVEAETAAAVAGRRLLKVSAWLYALLVAVIIFHPFS
                     AGRAEGRLRIDFLDVGQGDAALLTMPDGTTLLVDGGGRPGFNRRREGDAEAGRENFER
                     DTRGVGEAVVSEYLWWRGLDRVDYLLSTHADADHIDGLNDIARNFRVGAALVARTPAG
                     DSEFARFTETLRRASVPLYVVGRGDRLRFGSVEVDVLWPPVIAPGDRATSGNNDSVVL
                     RVRVGRRTFLLTGDIEAGAEAALVAASPGGLGSDAVKVAHHGSRTSSTEAFVAATRPT
                     FAVISVARDSPYGHPHESVVERWHAHGAQVLTTGERGMITVSTDGADLKVETFVKP"
     gene            complement(7376..8428)
                     /locus_tag="VF240_02510"
     CDS             complement(7376..8428)
                     /locus_tag="VF240_02510"
                     /inference="COORDINATES: protein motif:HMM:NF008607.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX7174145.1"
                     /translation="MVDEIRVGLIGDGMAARVFHAPIIQSVSRLRLKKVVARHGDASR
                     DRYPWVEVVRDAAALLQDEEIDLVVIATPNNSHFDLARQSLLANKHVVVEKPFTTSSA
                     QAQQLIDLARRQNRLLSVHHNRRWDGDFQTVKKLLGGNLLGHVVEYESRYDRFRNYPR
                     PNAWREEDGEGAGILYDLGSHLIDQAQVLFGLPRMVTADLSVQRSFAKAVDNFDLTLH
                     YDELKVILKAGMLVRERSPRFILHGTEGSFVKYGIDPQEEALKRGLTPAEPNWGEEPR
                     ELWGTLNTQIGGLHVEGRVETNAGCYQAYYQNIVDAIAGRAELEVKPTEAKDTIRIIE
                     SALRSNEQKCTLPLSS"
     gene            complement(8479..8709)
                     /locus_tag="VF240_02515"
     CDS             complement(8479..8709)
                     /locus_tag="VF240_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174146.1"
                     /translation="MQESGEIKELLIELRDVQREHLAEYRKVTQRSLELQQQAVTRQE
                     QLGNLYRRVVLACALLVMFIVIIIIYLMGRLS"
     gene            complement(8834..9133)
                     /locus_tag="VF240_02520"
     CDS             complement(8834..9133)
                     /locus_tag="VF240_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEX7174147.1"
                     /translation="MLAKRSKDQPRRSKERVAAFHPEFIEDLRYWVETDRKVALRAFD
                     LVEAVMRDPFTGLGKPEPLKYQLAGAWSRRLTQEHRIVYLVSEGRVDFLQARYHY"
     gene            complement(9114..9404)
                     /locus_tag="VF240_02525"
     CDS             complement(9114..9404)
                     /locus_tag="VF240_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7174148.1"
                     /translation="MPIQTTYTRARANLAKLCDEVAKNQEIVIINRRGSRDVALVSAE
                     ELSGLMETAHLLRSPKNADRLLTALKRAKAKRPKPKATVASLRKELGVGEKE"
     gene            complement(9489..>9668)
                     /locus_tag="VF240_02530"
     CDS             complement(9489..>9668)
                     /locus_tag="VF240_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174149.1"
                     /translation="YGYLIEEIIDSFTKRAIIPAIPLNHAASTNGRHPTANSAALIEN
                     LGGVGVECAAGDAGR"
BASE COUNT         1738 a         3146 c         3008 g         1776 t
ORIGIN      
        1 cacgacagac cgcgctcgcc gccgcgactc agccggggcc gcgcgtcaac attaacaagg
       61 cttcgcgcgc agagttggag cggctgccgg gcgtcgggcc ggcgacggcc tcgcgtatcg
      121 tcgaacaccg cgaacaatac ggccctttcc gcagggccga gcacctgttg ctcgtgcgcg
      181 gcttcagcga ctcacgcttc cgcgagctgc gccccctgat cgcggttgat tagagtagct
      241 tgtgtaatca ttgtgtgttg acgcgctgac agtaccgcaa gcggcactgt caggtgtgag
      301 ccgcccttcg aatcttgaac gaactctccg aactgcgccg cgccgcgcgc gaaatcttcg
      361 accacgcgct agccgagtcg gacgccggcc gcgccgtgcg ccgcgctgtc cggctcgatg
      421 gcgcacgtct gaacatcttc gacgatgaat tcgacctcga ccggccagcg cgcgtctatt
      481 ccgtcgctgc gggtaaggcc gcgcgcccga tggccgccgc gctcgacgag gcgctcggcg
      541 ctcgtctcat ccgcggcgtc gtctcggcgc cgctggcggg cgcaactctt tcgggccgct
      601 gggaggtcta cgcgggcgct cacccgctgc cgaacgcggc gagcctcgac gccgcgcgcg
      661 ccgcgttcaa tctcctccgc gaagcgaacc gccccgaagc gcttgtcctt ttcctcgtct
      721 ccggcggcgg ctcggcgatg attgaaagcc cgcgtgccgc cgacgtgacg ctcgaagatt
      781 tacgcgaggc caatcgcgct ctggtcgcct gcggcgcacc catcgccgaa gtcaacgcgg
      841 tgcggcgtgc gctatccgcg gtcaaggggg gtggcctgag cctgcgcgcg ccggcggccg
      901 cacaggtgac tttgatcgtc tccgacgtga acgccggctg tgagtcggat gtggcctcgg
      961 gcccgacgtt ccctcagccc gatgacgcgc gcgccgcttc gcacgtcgtc ggtaagtacg
     1021 gcctcggctc gcaactccct gcttccgtcc ttcgcctcat cgaacatcca ccggcagacc
     1081 ttgccgcgca agccgcaccc cgcgcactgc gccggcatca cgtgctgctc gacaatgcac
     1141 gcgccgtgca gagcgccgtc gagtcggcgc gcgcgcgcgg ccttatggcc gaggcgatgc
     1201 cggaactcgt cgagcagcac gtgtcggaag ggtcggccgc actcgtcgcg cgcctctcgg
     1261 agcttcaacg gcgggccggc gggcgtgtgg tgtgcctcgt gtcaggcggg gagttcgcgt
     1321 gccccgtgcg gggcgacggc attggcgggc gcaacgcgga gacggcgctc cgctgcgcgc
     1381 tcgaatttga gaagatggcc ggcgggccga gtcacgtcgt cgcgctgtgc gccggcacgg
     1441 acggcattga cggcaacagc cccgcggccg gcgccctcgc cgactcgacg acactcgccc
     1501 gcgcccgctc acgcggcctc gacccccacc gccacctcga agacagcgac gcccacacct
     1561 tcttccacgc cctcgacgac gcgctcctca ccggccccac gggaacgaac gtccgcgacc
     1621 tgcgtatact gctcacggaa taaagaagag gtcagaggtc agtgaagacg agcgtgtctt
     1681 tcactgacct ctaacctctg acctctgacc tctaacccct tccaaagtcg tcttcgaggc
     1741 gggtgatgtc gtcctcgccg aggtagtcgc cgcgctggat ttcgatgaag atgagggtgt
     1801 cggcgccggg gttctcgatg cgatgcgcgg cgcccacggg cacgtctatg gtctcgtccg
     1861 tgtggacggt gatctcgcgg ccgtcgagcg tgacgcggcc cgtgccggcg acgaccatcc
     1921 agtgttcggc gcggcgggcg tggcgctgat agctcagacg cttgccgggc aggacttcaa
     1981 tgcgtttgac cttgtagccc gcgccctcgt cgagcacggt gaagctgccc caggggcggc
     2041 ggtcgaagag cggcgaggtg tctgaagcat cgccctggtt catgcggttc ccttctcggg
     2101 tcgtgtcgga atcgtcgaga gcatttggaa tatagccgtt cggtcgcgcc gattcaaagc
     2161 gcgcgcggcg gcggcggctc acctgacgaa cgtttcgacg cgcaagtctt cgccgtccgt
     2221 gctgatggag acgaggccgc gcgcggcggt cgtcaacact tccgcgccgt gtgcgtgcca
     2281 gcgttccacc acggactcgt gcgggtgtcc gtaaggcgag tcgcgcgcga cggagatgac
     2341 ggcgaacgtg gggcgcgtcg cggcggcgaa cgcctcggtc gaagaggtgc gagtaatttt
     2401 ataggagaaa attttgctca gtacagttgt gtatgtatga cttacgggcc gaattacgta
     2461 ctgagcaaaa tcgattttgc tcagtacagg aagcccttcc tgtcgagtat gaagtcacaa
     2521 agcaacgggc cggtagccct gagagactac cggcccgccg tgttacttag cgagttcgtt
     2581 cactgacccg agacgaggaa cacgtcccac gcccagcgac cgagttgatg cccgcgcgcg
     2641 tcccttacct tcgcccggta cttgaactga ttgccgtgct ggtctgtccg ccgcgactct
     2701 ttgtaatcca agtcaatggt tctcagtccg aggtcaggca gaggatgtag ctcattcgct
     2761 tcagagacgc cattgtggtt tgtgtcctgc cacagacgca gggagtcgaa gatggcatcc
     2821 ctccggttga tgacgccgtc cgcgttcccg ccgctctcag gtttgtcgaa ctcggcgagt
     2881 gcgaggaagc cctgccgctc ttggcccgca ggcggtgtgg gctgcggggc gacgttgcca
     2941 aataactccc gccctccatc aatcgtcccg ttgccatcac ggtcgagcgc gagccatgcg
     3001 tcgtcagagt tagccgccgt ccatgaaatc ctcatcgggt ggccgctact tgagatgtcg
     3061 aaggtgacgc cgcccgccgc gtcggtgagg ttgaagccgt tgcccgccac gtcaatgacg
     3121 atgggcgaag gcggcgtgcc gtagcagccg cactgccact cgtcccagta ctgccagccc
     3181 tcggcacaca tgtactgctg ctccgggcac ggtgccgggg tctcgctaca gtaattatgc
     3241 gcatagttcc agtgccagcc gaactcgtaa catgactggg aagttgtcgg ggttgggcat
     3301 ccgaggcagg ccttctgttc gctcacgttc gaatcgtcgg gaaggctgta ggagaccgcc
     3361 catccgttgc tgggggccgc ggtgcaggta cactggcagg agctttgccc ctgaatgccg
     3421 ttggtgcaac tcgggcgcgc cgtgtggttc tggttcgagc agtctttgta ttcgaacacg
     3481 atgtcgcagc cggccccgcc cgggcgattc gtcgcggagc agcttctctt gatgcagtta
     3541 gacgtggttt gggcgaaggc ccaagtaaag aacgtcagca cgaacagcat gggcagcagt
     3601 attcttaact tcatcggcgt cctccttctg acagtcagaa atcaaggggt ggctcaggct
     3661 cgctcctacc ttgagggctg cctgctttgg cggagctgtt tctggagggc cgccgctccc
     3721 ttgtctttga ggacgccgag catccactgc ttgtagacca gtgctcccag caccgcctta
     3781 tcgttttcca gtgccttgtc ggcgggcaac tcgtcacccg agagcttccc gacgacggct
     3841 tcaaactgcg ccggggtgtc gtcgtacccg ctctccaccc agcgtttgta gatttccgca
     3901 cccgcacgct tgtcgcggag ctgcttgtag agtccggacg gatcggagcc gacggcgccc
     3961 gcctcctcca attcagcgaa ggcgaactcc actgacacgc tctcgatacc ccggccctct
     4021 tcgagcgtgt ccttcgtcag acgtgggata accttctcct catcgggcgc gaaggcccgc
     4081 acctttctgt cggccacgat gtccctgtgc agcctgtaat cgatgagctg ctctatctgg
     4141 cagttcgtct tgtcctcgta ggtcactgtc cagacgagtc cgagcgccgt gatgttttta
     4201 ccgctgagat tcttcgcccg gacggacgag ctgtcgaagc tgtccggcgt cgtctggtac
     4261 gacgcctcaa cgacttccag aaccctctgc ccggactccg acagaaaatc ttttttctgg
     4321 gcgaggtgag tcttgacctc ttgcgcgccc gccgcggcgg ctaggctcgt ccgcgacttc
     4381 ttcgagagag cgaaggccag cgtcaggccg agcgcggcca gaagcagtag gagcattgga
     4441 gatgcttttt tcatagtctt gtctccgtcg aaacgttagt ggggagtgaa gattacggag
     4501 tctgaggatg actacgtaaa cggggggcat tcgtgattgt cggactggca ggcgctatat
     4561 atctcggatt ccctctcaag tcaacgaata ttgttagaaa ttaataaccg cccctgccgc
     4621 tcacggtttc acgaacgttt ccaccttgag gtctgcgccg tccgtgctga cggtaatcat
     4681 gccgcgctcg ccggtggtga ggacttgcgc gccgtgtgcg tgccagcgtt ccaccacgga
     4741 ctcgtgcggg tgtccgtaag gcgagtcgcg cgcgacggag atgacggcga acgtggggcg
     4801 cgtcgcggcg acgaacgcct cggtcgaaga ggtgcggctc ccgtggtgcg cgaccttgac
     4861 ggcgtccgac ccgagaccac cgggcgaggc ggcgacgagc gcggcttccg cgccggcctc
     4921 gatgtcgccc gtcaggagaa atgttctccg gcccacgcgc acgcggagca cgacggaatc
     4981 gttgttgccc gacgttgccc tgtcgcccgg cgcaatgacc gggggccata acacgtccac
     5041 ttcgacactg ccgaagcgca acctgtcgcc gcgcccgacg acgtagagcg gcacactggc
     5101 gcggcgcagt gtctctgtga agcgagcgaa ctcggagtcg ccggccggcg tgcgcgcgac
     5161 gagcgcggcc ccgacacgga agttgcgcgc aatgtcgttg agaccgtcga tgtggtcggc
     5221 gtccgcgtgc gtggacagca ggtaatcgac ccggtcgagg ccgcgccacc agagatattc
     5281 ggagacgacc gcctcgccga cgccgcgcgt atcccgctcg aagttctcgc ggcctgcctc
     5341 ggcgtcgccc tcgcgtcgcc ggttgaagcc gggtcgccca ccgccatcga cgagcagcgt
     5401 cgtcccgtcc ggcatcgtga ggagcgcggc gtctccctgt cccacgtcga ggaagtcgat
     5461 tcgcaaacgc ccctcggcgc gccccgcgct gaacgggtgg aagatgatga cggcgacgag
     5521 taacgcataa agccatgccg agaccttcaa caatcgccgg cccgcaacgg cggcggccgt
     5581 ttccgcttcg acccgcaacg cgagtggcga ccagtgcgcg agggataaag ccagcacggc
     5641 gagcggcagg taatagagcg cgtagacgaa cgcagccgcg cccgcgtact cgggcagtcg
     5701 cacgcttgcg aggcccgccg cggcgaaggg gccggcgctg tccaccatca gacggttcgc
     5761 ccattcggcc agccacacaa gtggcgccgc cgcctttgct gacacttgcg cgacgaggac
     5821 ggcggcgagc gcggcgaacg agaggacggc catcagcgcg ccgacgaaga cgttcagcac
     5881 gagcgcggcc ggcgtgagcc ggtggaagta gagtacgagc gcgggcagca tccccgtttg
     5941 tacgcacgcc gagacgagta cggcggtgaa gacgtagcgg agcgccgcct gcaccctcca
     6001 ccgttcgagg cgggccgcga gcggcgtctt gaagagccgg tagctgtatg tcgagcgcct
     6061 catctcgcgg cgccagcggc gctcgctcca gtagagtgct tcggccagcc ggcgtaggag
     6121 cgcggggcac gcgggcgggt acggcgtcgt gctgcgtgga cgccacgcgc ccacctcgtg
     6181 caggcgcgtg agcgcgggcc acgcgagcac gacgattgaa aggacggaca ggaaggtgag
     6241 ttgaaacgag gggtcgaaga gggcgcgtgg gcgcgcggcg agcagcgcga gagcggcgcc
     6301 gccgagcgcg ttgagcgtcc cggcgggccg tccgatggcg ggcgcgagcg ccgcgactgt
     6361 gaacatcagc gccgcgcgca gcaccgaagc ttcggccccc accgcgaccg cataggccca
     6421 cacgcagacg gccgaggcgg cccactgcca cagccgccgc ctcgtcaaac ggcgcgccag
     6481 ccaccacgcc acgccgccga cgaaagtgat gtgtaaccca gagatgacga gaacgtggaa
     6541 cgtgccgccc tcgcgaaact tttcgccggt cgcgcgcgcg aggtgtagac ggttgccgag
     6601 caccgacgcc gcgagcacgc cggcggtgtc ggcggagaag ttgtccgtga agcgcgcgag
     6661 cagccacgcg cgccggcgtt cgagccagag aagcggcaga aagaccggct cgtcgtcgag
     6721 cctttcgacg acgagcggac tcttgatcgt cccgcgcgcg tccgcgtcgc ggcgttcgag
     6781 atactctgtg aacggtgaga cgccggggtt gcggtaacct tcgtcgcgat ccagtccgac
     6841 caacacacgc agccgcgccc cccgacgtaa ctccagcgcg tcatactccg cccacacctt
     6901 ttcgcttcgc gccggcgcga agagttccac cgcgcccgag acctcgcgct cctccccctt
     6961 gaagccccaa cgctccgcgc gcagacgcag cgccagcccg tccggcgcga cttcgggcgc
     7021 gcgctccagc acgcccgtca actccacggg gtcgcccccg ccgatgcgtc cctcttcaag
     7081 taactgccgc acgcgaggca cgcgcggcgt cgccacgccc tcggccgacg caaacaccgc
     7141 cccgcaagcc gcgaaggccg cgacgacgag aacggtcgca tgccccgcac gttcgcgaag
     7201 gcaagcgacg acggccagtg tcgagagaat tattccgccg gcgaagcacg cggcgagtgg
     7261 aggggagatt aaacgcgcga gaagaatccc ggccgcgaac gacgcggcga gcagcgccaa
     7321 cgggtgcagg ttgaacgacg gttggtgagg aaccaacatg agttacgtgt ttaattcacg
     7381 aagatagcgg aagcgtgcat ttttgttcgt tactccgaag cgccgattcg atgatgcgta
     7441 ttgtgtcctt cgcctctgtg ggcttaacct caagctcggc gcgaccagct atcgcatcaa
     7501 cgatgttctg atagtaagcc tgatagcaac ccgcattcgt ttctacgcgc ccctccacgt
     7561 gtaacccgcc gatttgcgtg ttgagcgtgc cccacagttc cctcggctct tcgccccagt
     7621 taggctctgc cggcgtgagt ccccgcttca gcgcctcttc ctgcggatca atgccgtatt
     7681 tgacgaagga gccttccgtg ccgtggagaa tgaaacgcgg actccgctcg cgcaccagca
     7741 tccccgcttt cagtatcacc ttcaactcgt cgtaatgcag tgtcaaatca aaattatcga
     7801 ccgccttcgc gaaactcctc tggacactga ggtccgccgt aaccattcgc ggcaggccga
     7861 acagaacctg cgcctgatcg atcaggtggg aaccgagatc gtacaggata cctgctcctt
     7921 caccatcctc ttccctccat gcgtttggcc tcgggtaatt tctgaagcga tcataacgcg
     7981 actcgtattc aaccacgtgg cccaggagat tgccgcccaa cagcttcttc actgtctgga
     8041 aatcgccatc ccacctccga ttatgatgga cgctgagaag tctgttttgc cggcgcgcca
     8101 gatcgatcaa ctgctgagcc tgagccgacg aagtggtgaa aggcttttcg accaccacgt
     8161 gcttattggc cagcaaagac tgtctggcaa ggtcgaaatg ggagttgttc ggggttgcga
     8221 tgacgaccag atcgatctcc tcatcttgca ggagggcggc ggcgtcacgc accacttcga
     8281 cccacgggta acgatcacgg gaggcgtccc catggcgcgc tacaactttc ttcagtctca
     8341 ggcgagaaac cgattgaatg atgggcgcgt ggaacacgcg ggccgccatg ccatcaccaa
     8401 taagccctac acgtatctca tctaccatct ggccaccgag actatgtata gctggaccaa
     8461 gccttaaatt gagggcgcct agctcaatct tcccatcaaa taaatgatga tgataacgat
     8521 aaacatcacg agtaacgcac acgccaacac gacgcgccgg tagaggttgc cgagctgctc
     8581 ctgacgcgtc acggcctgct gttgcaactc cagtgagcgt tgcgtcacct tccggtactc
     8641 ggccagatgc tcacgctgca cgtcacgtaa ttcgatcagc aattccttta tctcgcccga
     8701 ttcctgcatg atgactccga tgctggctaa cgcccggcct cacccgccgc gcacacaacc
     8761 tgatgagcgg cgagtcttgc ggatgagagc aacgctattc gcggtcgggt gcctgccgtt
     8821 ggtagatgcg gcgtcagtag tgataacgcg cctgaagaaa atcaactctt ccttcactaa
     8881 ccagatatac gattcgatgt tcttgcgtga ggcgtcgtga ccacgcgcct gccaactgat
     8941 acttgagcgg ttctggcttg ccaagacccg taaacggatc gcgcatcacg gcctcgacca
     9001 agtcaaaggc tctgagcgcg accttgcgat ccgtctccac ccaataacgt aaatcttcaa
     9061 tgaactcggg gtggaacgct gctacgcgct ctttgctcct gcggggttgg tctttactcc
     9121 ttttcgccaa caccaagctc cttacgcaaa gatgcgaccg tggccttcgg ctttggcctc
     9181 ttagccttag ctcgcttcag ggcagttaag agccgatccg cattcttcgg agacctgagg
     9241 agatgcgccg tctccatcaa gcccgacaac tcttcggcgg aaactaacgc cacgtcacgg
     9301 gagccgcggc ggttgatgat gacgatttcc tgatttttcg ccacctcatc acagagcttg
     9361 gccaaattcg cccgcgcgcg cgtatatgtt gtttggatag gcatgcagat cacctctgaa
     9421 gctaacgtac aggaacactg tacgtataaa ggcagattcg gtcaaggcga ggtagaaaag
     9481 ggaagcacct aacgcccggc atcacccgcc gcgcattcaa ccccgacacc acccaagttc
     9541 tcgatgagag ccgcgctatt cgcggtcggg tgcctgccgt tggtagatgc ggcgtgattt
     9601 aaaggtattg caggtatgat agctctctta gtgaacgaat caataatttc ttcgattaaa
     9661 tatccata
//
LOCUS       DASVES010000070        22328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1546617, whole genome shotgun sequence.
ACCESSION   DASVES010000070 DASVES010000000
VERSION     DASVES010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22328
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546617"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..598
                     /locus_tag="VF240_02535"
     CDS             359..598
                     /locus_tag="VF240_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7174150.1"
                     /translation="MAEVKLTRRNFLKGLAAPGLAGVSGTAGYASLLEPFNYELAETD
                     IPVPGLPESFDGFRIAQLTDVHHSRLVSVEKVRLR"
     gene            complement(973..1956)
                     /locus_tag="VF240_02540"
     CDS             complement(973..1956)
                     /locus_tag="VF240_02540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7174151.1"
                     /translation="MAAVMQETVLREQLFDRRQKLETAITASDDTLHLTGLLREVDDA
                     LERMGRGAYGLCEVCHDPVERERLIADPLVSVCLGCLSPVQRRALEDDLELAAQIQAT
                     LLPPQNFTSDGWQVSYHYEAAQHVSGDYIDLVGDDDGQLYFMLGDVSGKGIAASMLMT
                     QLHAMFRTLIPLGLPLNKIVERASRIFCESTLPTHFATLVCGRADTAGEVEVCNAGHL
                     PPLLIHEGRVTSLAATGMPIGVFCNEQFTSLRVSMAPGDTLLLYTDGLSEARDAADAE
                     YGAERLLKLVGDCLEHTPASLVTHCLKDLLAFSGDTPRTDDLTIMAIRRVG"
     gene            complement(2111..2503)
                     /locus_tag="VF240_02545"
     CDS             complement(2111..2503)
                     /locus_tag="VF240_02545"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7174152.1"
                     /translation="MTAKYKQMELLFKALADRTRLRLLNLMGADEVCVCYFVEVLGTN
                     QPKISRHLAYLRRAGVVAARREGKWMHYRVVAPADSHAARVFEEVRAWISDDREMQQD
                     RARLVRLCCAPQAPVRLQGAPRPASLAG"
     gene            complement(2620..3135)
                     /locus_tag="VF240_02550"
     CDS             complement(2620..3135)
                     /locus_tag="VF240_02550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174153.1"
                     /translation="MDFVIDEMRADDWGRVREIYLEGVATGEATFETGAPSWESWTAA
                     HHAFARLVARAGDTLLGWAALSPVSGRAVYRGVAEVSVYVGAGHRGRGVGRALLEALV
                     GASEATGVWTLQAGIFPENAASIALHTACGFRLVGTRERIGMRDGVWRDTVLLERRSR
                     TVGRDNAQDPP"
     gene            3292..3870
                     /locus_tag="VF240_02555"
     CDS             3292..3870
                     /locus_tag="VF240_02555"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX7174154.1"
                     /translation="MEDKTAETVKALKAHLALNVKSVERSIDFYRRLLGLEPSKVRAG
                     YAKFDVQNPLLNLTLNEVAFKERGALSHLGIQVASTDDVLATRDRWGRAGLLTRDEMQ
                     TNCCYAFQDKAWVADPDGNEWEVFVVLEDNLQESPACCGTTAAPEKIKLGEVETQTGE
                     SATCSTATVESTAAASSCCGGAASSEPVAISR"
     gene            3881..4606
                     /locus_tag="VF240_02560"
     CDS             3881..4606
                     /locus_tag="VF240_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX7174155.1"
                     /translation="MGADTKETFDLGRRAVAEFVGTALLLSAVVGSGIMGERLSGGNT
                     GVALLANTLATGAALVCLILTFGHVSGAHFNPAVTLSDAWAGGIGWRDVPAYVGAQVA
                     GAFIGVAAAHAMFGLPVFFASGRARAGGAQLFSEFVATFGLLAVIWGCVRARRAASAP
                     FAVGAYIMAGYWFTASTSFANPAVTLARSASDTFAGIRPIDVPGFVAAQLAGAAAATA
                     LMRWLIPSLPEAAGDIVMPHAKD"
     gene            4643..5071
                     /locus_tag="VF240_02565"
     CDS             4643..5071
                     /locus_tag="VF240_02565"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX7174156.1"
                     /translation="MMSERGRVLILCTGNSARSQMAEGLLRREAGARFEVLSAGTRPA
                     GVRPEAVEAMREVGVDISSHRSKSVDEFAGQAFDYVITVCDNARDNCPVFPGATERIH
                     WGFDDPAAVEGDEEARLAAFRRVRDEIATRLRAWLKEEEK"
     gene            complement(5172..5723)
                     /locus_tag="VF240_02570"
     CDS             complement(5172..5723)
                     /locus_tag="VF240_02570"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX7174157.1"
                     /translation="MRSTLTNIVLAAGLVAGTAAHAFAQDDRPAPPGWTQDGAGPRGE
                     RMGRRGGRARGRMARRHRMDLRGLRGQLDLTDAQREQLRALRQNARGANKDERAELRE
                     LRRLRRQGDTLTPEQQARAQALHEQLRANGEARRQQFLSVLTPEQRTRLEQQRREMRE
                     RREERRKRREEFRQRRTEQTPQP"
     gene            5952..7934
                     /gene="dxs"
                     /locus_tag="VF240_02575"
     CDS             5952..7934
                     /gene="dxs"
                     /locus_tag="VF240_02575"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7174158.1"
                     /translation="MAKRYLPRYNTRPNMKFLQAINAPADLRRLRQDDLQEVAAEIRN
                     YILETMSRVGGHTGASLGAVELAVALHYAFDTPHDRLVWDVGHQAYAHKILTGRREQL
                     HTIKQYGGLSGFLRREESEHDTFGAGHASTSLSAALGMAIARDQKGEDHHVVALIGDS
                     SLAGGMAMEAINQAGHLKTRLIVLLNDNEMSIAPAVGGLAGYLNRIREAQGYHRFKDE
                     VGDLLHDAPLIGERLYQAAKTVKDAIAAAVLPGALVNELGFKYVGYVDGHNTRALVRA
                     LEEAKQIKDGPVVVHALTQKGKGFPSREKNYYAWHATGPFDLKTGAAVKSSKPAPPLY
                     TAVFGESLCEEMASDPSVVALTAAMPDGTGIDKVLERYPERAFDVGIAEQHCVTFAAG
                     LSCEGLKPVCAIYSTFLQRAFDQIVHDVCLQNLNVKFCMDRAGIVGGDGPTHHGLLDI
                     AYLRGVPNIIAMAPKDEAEMRDMLHTMLQHTGPAAMRYPRGNGTGADIKRAPQVLEIG
                     KAEILRDAEAGGEVAIVAYGSMVHPALAAAEQLAREGVEATVVNARFVKPLDAGLLLA
                     LGRTKRLIVTVEEAYLAGGFGSAVMELLEENGLLDGVRIVRMGVPDRIITHGDPKLLL
                     AKYGLDADGIYTRVRECVEVLEERRANRRPSVHSRA"
     gene            8190..9008
                     /locus_tag="VF240_02580"
     CDS             8190..9008
                     /locus_tag="VF240_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174159.1"
                     /translation="MREQEQQFEPGRDPEATVIRPLFDEDAAEVARPVIPLDDRAMNE
                     GMGAYVETGTAPPAYTRAWKRRNHPTIMLVLVALFAGAVVGVTGLYFYQRSRTADTAV
                     KTAAPEQQQQAPATSSEAPPAATTTEAAQPSVVAESPAAAAEEVAAAPAASERERVRV
                     NDGEGTARETERAAPARRPEAEEEEARPAVVKRGRKGDDDDDERADASSPTRPRVYDT
                     QGPAPAGSAAERRAAREEDRLRRMRRERRRVRGEAYMVDSIRGIFEGGRNSPPR"
     gene            9378..10238
                     /locus_tag="VF240_02585"
     CDS             9378..10238
                     /locus_tag="VF240_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174160.1"
                     /translation="MPRATTRKRNATASKTSDIESLDLMRTGMPARDSIVKVWRHSKP
                     SGAKKTSGAKSMKAAASGVAAFRIIRTNEVDGYEKGATAEGTLAKMSAATPSAAAAND
                     NFTGTQRRAAKISIAKAPLRTYTDLKNLVRYLPTDESMIKRTPKIGKTATSDRVEEEM
                     RNVRVKVFLYAASRENDNDYHLILGRDPSKTPELYITMELSGLPPADSTAFAKLKAAR
                     DAFKAHFASDLPGKTYDFYDPPRPVEIEGSMFFDIGHATGTRPGPRSLKSRMPTVWEV
                     HPITKIVFNP"
     gene            10271..10498
                     /locus_tag="VF240_02590"
     CDS             10271..10498
                     /locus_tag="VF240_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174161.1"
                     /translation="MPRPKDKKKTKTAGPRGARALPPIDPLRRGMPALDSITGVKEVE
                     RDGHVFRIIKTNETDSYDEPARPVRKRRPKR"
     gene            10704..11456
                     /locus_tag="VF240_02595"
     CDS             10704..11456
                     /locus_tag="VF240_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX7174162.1"
                     /translation="MKRERIDKLLVERGLAPSRTRAQALVMAGAVLVNDQRVEKPSET
                     FLPDAALRVRGSDDPAARYVGRGALKLEKALAEFGVSVVGMTCLDVGASTGGFTDCLL
                     QHGARRVVALDVGHNQIDWRLRSDARVEVREGVNARHLRPADFDERFDLVTMDVSFIS
                     AAKVLPALKDLLAGGGRVVVLIKPQFEVGRGEVGKGGVVSDPAKHARVVAEVNASARE
                     LGFEVRGVVESPIRGADGNIEFLALYERAEVR"
     gene            11453..12124
                     /locus_tag="VF240_02600"
     CDS             11453..12124
                     /locus_tag="VF240_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174163.1"
                     /translation="MRKLSEAQALARLQREKAAFQSHVVKTYEHAARDCRTCPTAGVC
                     CTDAHFVNVHVTRLEAVAFRETLRRTPRLGAEDRRRVYARARETVALYSLRASGDTFA
                     QTFSCPLFEPGVGCLVHRRAKPAPCIQHACYDRWEDVPPVESQWRTERRVEQLNVETY
                     GSAWAWLPLPVWLTLVDPESDGAELARLTREWGARGSRPAHGNADARASQRPRRSLPV
                     LATRN"
     gene            12411..13289
                     /locus_tag="VF240_02605"
     CDS             12411..13289
                     /locus_tag="VF240_02605"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX7174164.1"
                     /translation="MSSIRRVGVLVKPHQPDALKTICRLVEWLAARGVTVMTGPKVIP
                     EEIERETGCAVETLEHEEMAAAADLVVVLGGDGTMIAAARMLGQREVPVLGVNHGSLG
                     YLTEYRVEELIPALESILEGRYAVERRMMLEAEVFRGEERLAAERVLNDVVISKSALA
                     RIIEIETRIGGHFVNRFRADGLIVSTPTGSTAYNLSAGGPIIYPTMGALVLTPICPHT
                     LSNRPLVVSDDMEIELTLKTDKEEVALTLDGQIGIPLEYEDRVTVRKSRTAFHLIQAD
                     TRNYFEVLRGKLRWGR"
     gene            13374..14852
                     /locus_tag="VF240_02610"
     CDS             13374..14852
                     /locus_tag="VF240_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX7174165.1"
                     /translation="MDDKDRISEAGRNAYERELTTDLKDQTPDRPRGMALHTRILIGL
                     LVGVVAGVVANRVLGGDHPGVAWTIRNITEPVGALFLRLLLMIVVPLVFSSLVVGVAG
                     IGDVRRLGRVGLKSFAYTFIISAISVVIGLTLANTIRPGERIDAATSQGLQARYGEDA
                     TKRVADATKTGTTDSVLMQVVKTIVPTNPVAAVAGIKAGEERQTDTPDMLHLMFFAVL
                     LGIAATMIPTEVSAPLLRGLQAVFEIAAKIIDIIMKVAPFAVACLLFNNTARFGLDLL
                     QALAWFVVTVLLGLAIHMFGVYSLSVYFLSRINPLEFFRRIKTVMLTAFSTSSSNATL
                     PTALRVSEENLGVPREINSFVLTVGATANQNGTALYEGVTVLFLAQLAGVDLTLTQQL
                     GIAYFAILGGIGTAGVPSGSIPFIILMLATYNIPTPLIAIILGVDRILDMCRTTLNVT
                     GDITAATYVARSEGYELLKPHVPELQRRGVVAGGPSSRDVLD"
     gene            14925..15179
                     /locus_tag="VF240_02615"
     CDS             14925..15179
                     /locus_tag="VF240_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174166.1"
                     /translation="MAELIHECETKVKDAGGVTYRAAVYGKERADGTWEGWIEFRPLA
                     GDALVLRTGQETSQPNRDALAYWASGLEPIYIDGAFSRAR"
     gene            15205..15510
                     /locus_tag="VF240_02620"
     CDS             15205..15510
                     /locus_tag="VF240_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174167.1"
                     /translation="MDKRKLAEIEEYVLGLFRSAGTGRLLAREIFDRSTEYANADIVR
                     AFEDLEKRQRLLVRHTEEGNDWVRLTPEGARVAGLPDTGDDDASHPSAPPHPPRSVP"
     gene            complement(15603..17399)
                     /locus_tag="VF240_02625"
     CDS             complement(15603..17399)
                     /locus_tag="VF240_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174168.1"
                     /translation="MKSLLAVVSSILVSAQPFAQTAPRRGTEGPAAGYWPEARSREIL
                     EKTQTVRLAPDLSHLSEGERRAVAKLLEAGKIFQSLYEEQRHERALSAERDLQRRTGP
                     DAQNLLTLYRLYQGPIATTLENKREPFLPVEMTPPGQNVYPWNITKEETDAFLAAHPE
                     RRDALLDLRTVVRRATAESLRRDLSELRQHPALSTLHPGLQAELQKMSARPDPKMLYA
                     VPYSVAYADQTVRAHALLNEAADAVQKDDEEFARYLRNRARDLLSDDYESGDASWITG
                     RFKNLNAQIGAYEVYDDALYGVKTFYAFSLLATRRAETEALRKAMSGIQALEDSLPYE
                     HHKKVREDIPVGVYDVIADFGQARGGNTATILPNENYLARRYGRTILLRANIMRNPDI
                     FQGAGNAWEAAVAPAHKDDLTSEGGFYRTLWHEVGHYLGVDATKTGRDLDTALQDASS
                     LLEEMKADLVALYVAEALRKQNYYTDAQLRSVYAGGVSRVLQNNRPRREQPYNTMQLM
                     QWNFFLEQGLLSFDPQTKKLRIDYGKYHDVVGKLLRKVLEVQREGDRAAADAFIDRYA
                     KWDDSLHGAVAAGIRAQQRYRFRIFKYAALGE"
     gene            17667..18413
                     /locus_tag="VF240_02630"
     CDS             17667..18413
                     /locus_tag="VF240_02630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7174169.1"
                     /translation="MIFPIGDDNTDRATTPLVNYVLIAINVLVFVFAQGLGSNDQFTY
                     AWATVPQEIVTNTDIAQNVPLGVDPATNRPVSIPLQPIPVSVYFTLFTSMFMHGGFMH
                     LFGNMLYLWIFGDNLEDRLGHVRYLLFYLLCGVIASLSHVLMNTNSFIPSLGASGAIS
                     GVLGGYLLLFPRRQVRVIMLRMLTTVPAYVALGIWILFQFFEGYMNRGAAGGGVAYAA
                     HIGGFIAGLLLIKIFAIGRGGGAPSVARTV"
     gene            18623..19069
                     /gene="queF"
                     /locus_tag="VF240_02635"
     CDS             18623..19069
                     /gene="queF"
                     /locus_tag="VF240_02635"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX7174170.1"
                     /translation="MSVTDATLPRVNQQGLDIRPTPREEMNLYPLDTFPYEYAGREIH
                     IKFDIPEFTCVCPFSDFPDFATIRLDYVPNERCVELKSLKLYVNSFREVKVFHEHVIN
                     VILEDFVRACDPLAVEIEGDFNVRGNIKTVVSASYKKSGVRSQEPE"
     gene            19131..19376
                     /locus_tag="VF240_02640"
     CDS             19131..19376
                     /locus_tag="VF240_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174171.1"
                     /translation="MSLSIIAILAIVLGVVAVVGFFVLKRVLRWAVRLFLLGVAVVLL
                     LAGAVMWWWYAPAGGDAPARQNRNGGGGATTRPANRR"
     gene            19399..20706
                     /locus_tag="VF240_02645"
     CDS             19399..20706
                     /locus_tag="VF240_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7174172.1"
                     /translation="MSKREEDFADPIEEVARAARARGAGGIWGRYRRLPTNVFAISSV
                     SLLNDASSEIVYPFLSVFLTTLGASALSMGLIEGAAESVSSFLKLFAGYFSDRLGRRK
                     GLVVFGYALAGTMRTLLGFATSWTHVLAIRLADRVGKGIRSAPRDAMIADAAAPDERG
                     LAFGLHRAMDHAGAVIGPLFGFAIIALIAQDRSAPSLGELRTLFLLAAVPALAAVLVA
                     ALFVRDAPREVKTDLREVSPPPRLSLRGFDTNFKRFLVVLALFTLANSSDLFVLRRAQ
                     EAGVGAATIPLLWAALHACKVLTSLVGGDLSDRLGRKRMIVSGWILYACVYAGFAFVS
                     RPGEAWALFLLYGVYAGLTEGAEKALVADLVPAEQRGTAYGLYNLAFSITVLPASLLT
                     GALWNFYGARTAFLASAAVATFAAGLLLLAVRTSRWDARAPQL"
     gene            20808..>22328
                     /locus_tag="VF240_02650"
     CDS             20808..>22328
                     /locus_tag="VF240_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX7174173.1"
                     /translation="MSSTDYRLITDITEVSAACEELSRHGAVGFDCETTSLDPYGGRL
                     RLVQIASPDGVCVFDLFALAPDGHADKVEALEPLRRLLAATKPVKIAHNAKFDAKWVR
                     HALGVELHGDFDSRAGGNGANGEGGTVERAGLFDTLLASQIISAGETEDRHGLAAVVE
                     RYLGETVDKSQQVSNWGGELSEAQLRYAARDAAVMLPLRDKLITQLRANNLGRCAQLE
                     FECVVPVASMELAGVYLDAACWREQLAVVEKMRKTLAEELQDMLAEESPQQSFFGPTR
                     VELNLDSHVQLTKALTRLGVPVPDSTRNWKLQPLANQYPSVAKLLEYRTVQKALTSYG
                     ENILKEINPTTGRIHANFHQIGAPTGRFACTNPNIQQVPHSIEYRRCFRAPEGKKLII
                     SDYCVAAGTRVATRRGLVKVESVRVGNEVLVEDGSAQRVSATVSRGRLPVVTVTLKNG
                     YALTATDLHRIRVIDEEGRYVWRRIGELRRTDCVAVQPGRGLHDELSYVELPEAVSE"
BASE COUNT         3776 a         7396 c         7296 g         3860 t
ORIGIN      
        1 gctggagatg cacgtccgtg ccgcagacct cgctcaactc aacgtcgagc atcgccgagt
       61 taggctcaag ctccggctcg tcaagttcgc gcacttcgac cgctgcgtgc ggcgcgggga
      121 tgagggcggc tcggactttg gtcatcggtg ttcggctacg tcgtgtgggc tacggttggg
      181 aagggatgag ggatgaaaga aggtagtaag cagtgagcag tgagcagtaa gcagtcaatg
      241 ccggtcgagc gccttgactg ccgagtgccc gctgcctcct gccgactcgc cttcatccct
      301 catccttcat ccttgcctcc ctacgccctg agttggcgat gtttcaattt ccggcgcgat
      361 ggcggaagtt aaactcacgc ggcggaattt tctgaagggg ctggcggcgc ccggtctcgc
      421 gggcgtctcc ggcacggccg gctacgcgag ccttctggag ccgttcaact acgagctggc
      481 tgagaccgac atccccgtcc ccggcctgcc cgagagcttc gacggtttcc gcatcgccca
      541 actgacggac gtgcatcaca gccggcttgt ctccgtcgag aaggtgagat tgcgttagtc
      601 gccattaagc attttattcc aatcgtatct gtggcgaccc aaaaggttat cctgcatctg
      661 cttcgcggcc tccttggatg aacagacgcg tgcgcgatag tacaaatacc agccctcgcg
      721 taagtgttcg ttaatagttt ttgacagatg tgagccgttt ctggcatagc aggcgattcg
      781 tctatgttcg ttgcctgtct cacccacata atgccattga atgaatcccc ccttgggcct
      841 ccagtcccga gttcatagca agcagggcca tcgtaatcaa aggaatcgtc aaaccactct
      901 ctagcagagg caatcaaccc aggttctgtc catcgtccca tgatttacac ctctcattta
      961 gctctcatgc gcttacccca cgcgcctgat ggccatgatc gtgaggtcgt ccgtccgcgg
     1021 cgtgtcgccg ctgaaggcga ggaggtcttt gaggcagtgg gtgacgagcg aggccggcgt
     1081 gtgctcaaga caatcgccga ccagttttag caggcgctcg gcgccgtact cggcgtcggc
     1141 cgcgtcgcgc gcctcggaga gtccgtccgt gtagagcaaa agcgtgtcgc cgggcgccat
     1201 cgagacgcgc agggaagtga actgctcgtt gcagaagacg ccgatgggca tccccgtcgc
     1261 cgcgagcgac gtgacgcggc cttcgtggat gagaagcggc ggcaggtggc cggcgttgca
     1321 gacttcgacc tcgcccgccg tgtcggcccg gccgcagacg agtgtggcga agtgtgtggg
     1381 cagcgtgctc tcgcagaaga tgcggctggc gcgctcgacg attttgttga gcgggaggcc
     1441 gagcgggatg agcgtgcgga acatcgcgtg gagctgcgtc atcagcatcg aggcggcgat
     1501 gcccttgccg gaaacgtcgc cgagcatgaa atagagctgg ccgtcgtcgt cgccgactaa
     1561 gtcgatgtag tcgccgctga cgtgctgggc cgcctcgtag tggtaggaga cctgccagcc
     1621 gtcggaggtg aagttttgcg gtgggaggag cgtggcctga atctgtgcgg ccagctccag
     1681 atcgtcttcg agcgcgcgcc gctgcacggg gctgaggcag cccagacaga cggagacgag
     1741 cgggtcggcg atcaggcgct cgcgctcgac ggggtcgtgg cagacctcgc acagaccgta
     1801 tgcgccccgg cccatacgct ccaacgcatc atctacctcg cgcagaagtc cggtcaggtg
     1861 aagggtgtcg tcggaggccg tgatggcggt ctccagtttt tgtcggcggt cgaacaactg
     1921 ctcgcggagc actgtctcct gcatgacggc ggccatgttc atagctcctt gcggtctgtg
     1981 gtcagacgtt gaaaagtgtc aatcgcgagg gtcgttttcg tcggcgtaat tataccaccg
     2041 gcgcgtccta gcctcaacac attgttgcgc gtctgaaacg aagcgggtat cacgattttc
     2101 gatgattttc ctatcccgcg aggctggccg ggcgcggcgc gccttgcagg cggacgggcg
     2161 cttgcggcgc gcagcacagg cgcacgagtc gggcgcgatc ctgctgcatc tcgcggtcgt
     2221 cgctgatcca tgctcgcacc tcctcgaaga cgcgcgccgc gtgtgagtcc gccggcgcga
     2281 cgacgcggta gtgcatccac ttgccctcgc gccgcgcggc gacgacgccg gcccgacgca
     2341 gataggcgag gtggcgcgaa atcttcggct ggttcgtccc cagcacctcg acgaagtagc
     2401 agacgcagac ctcgtcggcg cccatcaggt tcagcaggcg caggcgcgtc cggtcggcga
     2461 gcgccttaaa gagcagttcc atctgtttgt acttagccgt catcacaacc cctcgcaaaa
     2521 aattctcact actcaacacc cccgactcca tcttatcttt gcgcttttgc gtgaaccttt
     2581 tcttcgagac gagaaatctc acgcaaaggc gcgggaggct caaggcgggt cttgagcatt
     2641 atcgcgcccg accgtgcgac tccgccgctc aagcagaaca gtgtcgcgcc agacgccgtc
     2701 gcgcatcccg atgcgctcgc gcgtgccgac caagcggaag ccgcaagcgg tgtggagcgc
     2761 gatgctcgcc gcgttctccg ggaagatgcc ggcctgaagc gtccacaccc ccgtcgcctc
     2821 cgacgccccg acgagtgctt cgagcagcgc gcggcccacg ccccgacccc gatgccccgc
     2881 gccgacgtag acgctgacct cggcgacgcc gcggtagacc gctcggcccg acacggggct
     2941 gagcgctgcc cagccgagaa gagtgtcgcc cgcgcgggca acgagacgcg cgaaggcgtg
     3001 gtgcgccgcc gtccaactct cccacgacgg cgccccggtc tcgaaggtcg cctcgccggt
     3061 ggcgacgcct tcgagataga tttcgcgcac ccggccccag tcgtccgcgc gcatttcgtc
     3121 gatgacaaaa tccatatcgc cgccggcaac catccgttag gacacagcac gcgccccacc
     3181 cgtcaaaaaa aaatcaaccg tcgttacaaa tacgtcttga cacatatatc ggatgcatgt
     3241 aggatgcgct tacattcgcc aaggcgaata tgattgtaaa ggagaaacga gatggaagac
     3301 aagactgcgg agacggtgaa ggcgctcaag gcgcatctgg ccctaaacgt gaagagcgtg
     3361 gagcggagca tcgacttcta ccggcgtctg ctggggctgg agccgtcgaa ggtgcgcgcc
     3421 ggctacgcga agttcgacgt gcagaacccg ctgctcaacc tgactttaaa cgaggtcgcc
     3481 ttcaaagagc gcggcgcgct ctcgcacctc ggcatccagg tggcctcgac cgacgacgtg
     3541 ctcgccacac gcgaccgctg gggccgcgcg gggttgctca cgcgcgacga gatgcagacc
     3601 aactgctgct acgcctttca ggacaaggcg tgggtcgccg acccggacgg caacgagtgg
     3661 gaggtcttcg tcgtgctcga agacaacctg caagagtctc ctgcctgctg cgggacgacg
     3721 gccgcgcccg agaaaatcaa actcggcgag gtcgagacgc agacgggcga gtccgccacg
     3781 tgctcgacgg cgaccgtcga atccacggcc gccgcttctt cgtgctgcgg cggggccgcg
     3841 tcgtccgaac ccgtcgccat ctcgcgctga aagagtgtcg atgggggcgg atactaaaga
     3901 gacgtttgac ctcgggcgca gggccgtggc cgagtttgtg ggcacggccc tgctgctgtc
     3961 ggcggtcgtc ggctcaggga tcatgggcga gcgcctgagc ggcggaaaca caggcgtggc
     4021 gctgctggcg aacaccttgg cgaccggcgc ggcgctcgtc tgtctgatcc ttacgttcgg
     4081 ccacgtctct ggcgcgcact ttaacccggc cgtgacgctc tccgacgcgt gggccggcgg
     4141 catcggctgg cgcgacgtgc cggcttacgt cggggcgcag gtggcgggcg cgttcatcgg
     4201 ggtggccgcc gcgcacgcga tgttcggcct gcccgtcttc ttcgcctcgg gccgggcgcg
     4261 cgcgggcggg gcgcagctct tcagcgagtt cgtcgcgacg ttcggcctgc tggcggtcat
     4321 ctggggctgc gtccgcgcgc gtcgcgccgc gtcggcgccc ttcgccgtcg gggcgtacat
     4381 catggccggc tactggttca ccgcctcgac ctcgttcgcc aacccggccg tgacgctggc
     4441 gcgctccgcg agcgacacgt tcgccggaat tcgccccatt gacgtgcccg gcttcgtcgc
     4501 cgcgcaactc gccggcgcgg ccgcggcgac ggcgctgatg cgctggctca ttccctcgct
     4561 gccggaggcg gccggcgaca ttgtcatgcc gcacgcgaaa gactgagcgc gcagtgcgga
     4621 cggagtttga gaagggaagg cgatgatgtc ggagagaggt cgcgttctga ttctttgcac
     4681 gggcaactcg gcgcgcagtc agatggccga ggggctgttg cggcgtgagg ccggcgctcg
     4741 tttcgaggtc ttgagcgcgg ggacgcggcc ggcgggagtg cggcccgaag ccgtcgaggc
     4801 gatgcgcgag gtgggcgtgg acatctcgag ccaccgctcg aagtccgtgg acgagttcgc
     4861 cgggcaggcg ttcgactacg tcatcacggt ctgcgacaac gcgcgggaca attgcccggt
     4921 tttccccggc gcgaccgagc gcatccactg gggcttcgac gacccggccg ccgttgaagg
     4981 cgacgaggag gcgcgtctcg ccgccttccg ccgcgtccgc gacgagatcg caacgcgcct
     5041 gcgcgcgtgg ctgaaagaag aagagaaata aatgtgcccc ggcgattacg tcggcacaaa
     5101 gaaaaggaag gcggcccgcc ttgacgaaaa ggtgggccgc cttccttttg tttcttgtcc
     5161 gtcagagtcg cttacggctg cggcgtctgc tccgtgcggc gctggcgaaa ctcttcgcgg
     5221 cgtttgcggc gctcctcgcg gcgctccctc atctcgcggc gctgctgttc gagccgcgtg
     5281 cgctgctcgg gcgtgagcac ggagaggaac tgctgccggc gtgcctcgcc gttcgcgcgc
     5341 aactgttcgt gcagggcttg cgccctggcc tgctgctcgg gcgtgagtgt gtcgccctgc
     5401 cggcgcagcc gacgcaactc gcgcagctcg gcgcgctcgt ccttgttggc cccccgcgcg
     5461 ttctgccgga gggcgcggag ctgttccctc tgcgcgtccg tcaggtcgag ctggccccgc
     5521 aggccgcgca agtccatgcg gtgtctgcgg gccatgcgcc cgcgggcacg gccgccgcgc
     5581 cggcccatac gctcgccgcg cggcccggcg ccgtcctgcg tccagcccgg cggcgcgggc
     5641 ctgtcgtcct gcgcgaatgc gtgggcggcc gtgcccgcga cgaggccggc tgccagaacg
     5701 atgttggtaa gagtgcttct catggtcatt taaattcccc tctttgaatt gattacctgc
     5761 gcgcggcgcg gcgctgggct tcagacgttg ccctgatacc gggagacccg tccgcggcgc
     5821 ttgttgttct gaacctttta gacgcgcggc acgttattct ggccgtggta tttttgaagg
     5881 gagtcgtggt cgaagttctc ggatgagcat tttatcgtga cacttcgccc acgttttgtg
     5941 actacttgtt gatggcaaaa cgctacctgc cgcgttacaa tacgcgcccg aacatgaaat
     6001 tcttacaagc aatcaacgcg cccgccgacc tgcgccggct ccggcaggac gacctgcaag
     6061 aggtcgccgc cgaaatccgc aactacattc ttgagacgat gtcgcgtgtg ggcggccaca
     6121 ccggcgccag cctcggcgcc gtcgaactcg ccgtcgcgct ccactacgcc ttcgacacgc
     6181 cgcacgaccg actcgtctgg gacgtgggcc atcaggctta cgcgcacaag attctcacgg
     6241 gtcgccgcga acagctccac acgatcaaac agtacggcgg gctgtcaggc ttcctgcggc
     6301 gcgaggagtc ggaacacgac accttcggcg cgggccacgc ctcgacctcg ctctccgccg
     6361 cgctcgggat ggcgattgcg cgcgaccaga agggcgagga tcatcacgtc gtcgcgctca
     6421 tcggcgactc aagtctggcg ggcggaatgg cgatggaggc catcaatcag gcgggccacc
     6481 tcaagacgcg tctgatcgtg ctcttaaacg acaacgagat gtccatcgcc ccggccgtcg
     6541 gggggctggc cggctatctc aaccgcatcc gcgaggcgca ggggtatcac cgcttcaagg
     6601 acgaggtggg cgacctcctg cacgacgcgc cgctcatcgg cgagcgtctt taccaagccg
     6661 ccaagaccgt caaggacgcc atcgccgcgg ccgtgctgcc cggcgcgctc gtcaacgaac
     6721 tcggcttcaa gtacgtcggc tacgtggacg gccacaacac gcgtgcgctc gtgcgcgcgc
     6781 tcgaagaggc caagcagatc aaggacggcc ccgtcgtcgt ccacgcgctg acgcagaagg
     6841 gcaagggctt cccctcgcgc gagaagaact actacgcgtg gcacgccacc ggccccttcg
     6901 acttgaagac cggcgcggcc gtcaaatcgt cgaagccagc gcccccgtta tacacggcgg
     6961 tcttcggcga gtcgctgtgc gaggagatgg cgagcgaccc gagcgtcgtc gcgctgacgg
     7021 ccgcgatgcc cgacggcacc ggcatcgaca aggtgctgga gcgatacccc gagcgcgcct
     7081 tcgacgttgg catcgccgag caacactgcg tcacgttcgc cgccgggctg tcctgcgagg
     7141 gcctcaagcc ggtctgcgcc atctactcga ccttcctcca gcgcgccttc gatcaaatag
     7201 tccacgacgt gtgcctgcaa aacttgaacg tcaagttctg catggatcgc gccggcatcg
     7261 tcggcggcga cggccccaca caccacggcc tgctcgacat cgcctacctg cgcggcgtgc
     7321 cgaacatcat cgcgatggcc cccaaggacg aggccgagat gcgcgacatg ctccacacca
     7381 tgctccaaca cacggggccg gcggcgatgc gctacccgcg cggcaacggg acgggcgccg
     7441 acatcaaacg cgcgccgcaa gtgttggaga tcggcaaggc cgaaatcctg cgcgacgcgg
     7501 aagccggcgg cgaggtcgcc atcgtcgcct acggctcgat ggttcacccg gcgctcgcgg
     7561 cggccgaaca actggcgcgt gaaggcgtcg aggccacggt cgtcaacgcg cgcttcgtca
     7621 agccgctcga cgccggattg ctgctcgcgc tcgggcgcac gaagcgtctc atcgtgacgg
     7681 ttgaggaggc gtatctggcg ggcggcttcg gctcggcggt gatggagcta ctggaagaga
     7741 acgggctgct cgacggcgtg cggatcgtcc gcatgggcgt gcccgaccgc atcatcacgc
     7801 acggcgaccc caagctgctg ctcgccaaat acggcctcga cgccgacggc atctacacgc
     7861 gcgtgcgcga gtgcgtcgaa gtgctggagg agcgccgcgc caaccgccgc ccttccgtac
     7921 acagccgggc ttagggcagc ggacttctgt ttcaaaagag ggcgcgtcgg ctcacggggc
     7981 cgacgcgccc ttcgtctttc aagtcagaga atttggggcc gcaccgtgcc gcgccgtgtc
     8041 acgcgacgac ccttacggcc tctgttgtca aagatttaat cgaaccgctg tcacagtttt
     8101 gtcatcgaac tatcgtggaa agtaccgaat agcggtctca agtttgcatc aaataaattg
     8161 ccggtagaag tttgccaaca agaacgtcga tgagagaaca ggaacaacaa ttcgagccgg
     8221 ggcgcgaccc ggaggcgacc gtcatccggc cgctctttga cgaagacgcc gccgaggtgg
     8281 cgcgccccgt cattccgctc gacgaccgtg cgatgaacga agggatgggc gcctatgtcg
     8341 agaccggcac ggccccgccc gcctacacgc gcgcctggaa gcgtcgcaac cacccgacaa
     8401 tcatgctcgt cctcgtcgcc ctgttcgccg gggccgtcgt cggggttacg ggtttgtact
     8461 tctatcagcg cagccgcacc gccgacaccg ccgtcaagac ggccgcgccg gagcaacaac
     8521 aacaagcgcc tgccacttcc tcagaagcgc cgcccgcagc gacgaccact gaggccgcgc
     8581 agccttccgt agttgccgaa tccccggcgg cggccgcgga agaggtggcg gccgcgcccg
     8641 ccgcgtcaga gcgtgagagg gtcagggtca acgacgggga gggcacggcg cgtgagacgg
     8701 aacgtgcggc ccccgcgcgc cggcctgaag ctgaagaaga agaagcgcgc ccggcggtcg
     8761 tcaagcgtgg caggaagggc gacgatgatg acgacgagcg ggccgacgcg tcgtcgccga
     8821 cgcgtccgcg cgtttacgac acgcaggggc cggcgcccgc cggctccgcc gccgagcgtc
     8881 gcgccgcgcg cgaagaagac aggttgcgac gaatgcgccg ggagaggcgc cgcgtgcgcg
     8941 gcgaggccta catggtcgat agcatcagag gaatttttga aggcggtcgg aattctcctc
     9001 ctcgctaaca tgcacaaggg ggagaaaaca gggaggggcg tgatggcgta agctttctcg
     9061 ctcctccctt tttcttttgc accgacgctc ccgccctgcg cgcgcgaccg cagcggcgct
     9121 tatagcagtt tgcatttgct tgtgagtcat ccgtgctccc ctcgccagtc gcacagatgg
     9181 acgcaatcca ccagcacagg aatataaacg cgcacgcgcc gaacagcttt cgagagacgg
     9241 gccacatgtc gtatagtgtc gccgtctcga actcttcacg tttcctcggc gccgtccccg
     9301 tcacctcgcc ccggcccccg cgctatttga aatagcccgg ctcacaaaat ccgcaacgac
     9361 taaggagcat ccccgccatg ccacgagcca caaccagaaa aaggaacgcg accgcttcaa
     9421 agacctccga catagaatcg ctcgacctga tgcggacggg tatgcccgca cgcgactcga
     9481 ttgtcaaagt gtggcggcat tcaaaaccgt cgggcgcgaa gaagacgtcg ggggcgaagt
     9541 cgatgaaggc ggcggcctca ggcgtcgccg cgtttcgcat catccgcacc aacgaggtgg
     9601 acggctacga gaagggcgcg acggccgaag ggactctggc gaagatgtcc gccgccaccc
     9661 cgtccgccgc cgccgccaac gacaacttca ccggcaccca gcgccgggcc gccaaaatct
     9721 ccatcgccaa ggcgccgctc aggacttaca cagacctgaa gaacctcgtc aggtatctgc
     9781 ccacagacga atccatgatc aagcgcaccc cgaagatcgg gaagacggcc acctcggatc
     9841 gcgtcgaaga ggagatgcgt aatgtgcggg tcaaggtctt cctctacgcc gcgagccgcg
     9901 agaacgacaa cgactatcac ctcatccttg ggcgcgaccc ttcgaagacg ccggagttgt
     9961 acatcacgat ggagctttcc ggcctgccgc ccgcggacag cacggcgttt gccaaactca
    10021 aggccgcgcg cgacgccttc aaggcacact ttgccagtga cctgcccggc aagacctacg
    10081 atttctacga cccgccgcgg cccgtggaga tcgaagggtc gatgttcttc gacatcggcc
    10141 acgccacggg gacgcgaccc ggccccagga gcttgaagag ccggatgccg accgtctggg
    10201 aagtgcatcc gatcacgaag atcgtcttca acccgtaaag cgcccccgcg cgccggctga
    10261 aggagagcga gtgccgcgac cgaaggataa gaagaagact aagaccgccg ggccgcgcgg
    10321 cgcgcgtgcg ctcccgccca tcgacccgct caggcggggt atgccggcgc tcgattccat
    10381 caccggcgtc aaggaggtcg agcgcgacgg gcacgtcttc cgcatcatca agaccaacga
    10441 gacagactcc tacgacgagc cggccaggcc cgttcggaag cgcaggccga agcgttgaac
    10501 gatcagaacg atccttcgca cgcagtcgcg gaggagaagg cgggtggtcg gggaacacgg
    10561 atgaaacgga tgagacggat gagcacggat tcgtgccgat ccgttttcat ccgtctcatc
    10621 cgtgttcccc ggccgcgcgg cgacgcccga cgcacatacc cccacttgtg cccccgcgca
    10681 ctccctctta agattcttcg gacgtgaagc gcgagcgcat cgacaagctg ctggtcgagc
    10741 gcgggctggc cccgtcgcgg acgcgcgcac aggcgctcgt catggccggc gccgtgctcg
    10801 tgaacgacca gcgcgtcgag aagccttcgg agacgtttct gccggacgcc gccttgcgcg
    10861 tgcgcggcag cgacgacccg gccgcgcgct acgtcggccg cggcgcactg aagctggaga
    10921 aggccctcgc cgagttcggc gtaagcgtcg tcgggatgac ctgtctcgac gtgggcgcct
    10981 cgaccggcgg cttcaccgac tgtctcttgc agcacggcgc gcgccgcgtc gtcgccctgg
    11041 acgtggggca caaccagatc gactggcgcc tgcgctcgga cgcgcgcgtc gaggtgcgcg
    11101 agggggtgaa cgcgcgccac ctgcgccccg ccgacttcga tgaaagattc gacctcgtca
    11161 cgatggacgt gtcgttcatc tcggcggcga aggtgctgcc ggcgctgaaa gatttgctcg
    11221 ccgggggcgg gcgcgtcgtc gtgctcatca agccgcagtt cgaggtgggg cgcggcgagg
    11281 tcggcaaggg cggcgtggtg agcgaccccg caaagcacgc gcgcgtcgtc gcggaggtga
    11341 acgcgtcggc gcgtgagttg ggcttcgagg tgcggggcgt cgtcgagtcg ccgatccgcg
    11401 gcgcagacgg caacatcgag tttctggcgc tctacgaacg ggcggaggtg cggtgaggaa
    11461 actttcagag gcgcaggcgc tcgcgcggct ccagcgcgag aaggcggcct tccagagcca
    11521 cgtcgtcaaa acttacgagc acgccgcgcg cgactgccgg acgtgcccga cggcgggcgt
    11581 ctgctgcacc gacgcccact tcgtcaacgt ccacgtcacg cgtctcgaag ccgtcgcctt
    11641 ccgagagacg ctccggcgca cgccgcgtct cggcgcggaa gaccggcggc gcgtctacgc
    11701 gcgggcgcgc gaaacggtcg cgctctactc cctgcgcgcc tcgggcgaca ccttcgcgca
    11761 gaccttctcg tgtccgctgt tcgagccggg cgtcgggtgt ctcgtccacc gccgcgccaa
    11821 gcccgccccg tgcatccagc acgcctgcta cgaccgctgg gaggacgtgc cgcccgtcga
    11881 gtcgcagtgg cggacggagc gccgcgtcga gcagttgaac gtcgagacct atggctccgc
    11941 gtgggcgtgg ctccctctgc ccgtctggct cacgctcgtt gaccccgagt cggacggggc
    12001 cgaactcgcg cggctcaccc gcgagtgggg cgcgcgcggc tcacgccccg cacacggcaa
    12061 cgccgacgcc cgcgcctcac aacgcccgcg ccgctcgctc cccgtcctcg ccacccgcaa
    12121 ctgacatcag agatgaatgg acaggattaa caggactgac aggatgaaga tgatttgata
    12181 tctcaaatct caaatctcaa atctcaaatc tcaaatctca aatctcaaat ctcaaatcaa
    12241 tcctttcttc gtcctgtccg tcctgcgaat cctgtcgatc ctgtctaaag tttcgcgaag
    12301 ccgtctgaac ggtcgcgcac ataagcgttg acacccccgc gcgggcgcgg ctatcatccg
    12361 agccgatttc ccgtgaaaat tcaaacactg ccgtgggcac cgagaggctt atgtcttcga
    12421 ttcgacgcgt cggggtcctg gtcaagccgc accagccgga cgcgctgaag actatctgcc
    12481 ggctggtcga gtggctggcg gcgcgcggcg tgacggtgat gacggggccg aaggtcatcc
    12541 ccgaggagat cgagcgcgag acggggtgcg ccgtcgagac gctggagcac gaagagatgg
    12601 cggcggcggc cgacctcgtc gtcgtcctcg gcggcgacgg gacgatgatc gcggcggcgc
    12661 gcatgttggg gcagcgcgag gtgccggtgc tcggcgtcaa ccacgggagc ctcggctacc
    12721 tgaccgagta tcgcgtcgag gagctgatcc cggcgctcga atccatcctc gaaggccgct
    12781 acgccgtcga gcggcggatg atgcttgagg cggaggtctt tcgcggcgag gagcgactgg
    12841 ccgcggagcg cgtgctgaac gacgtggtca tcagcaagtc ggcgctggcg cgcatcatcg
    12901 agatcgagac gcgcatcggc ggccacttcg tcaaccgctt ccgcgccgac gggctgatcg
    12961 tctccacccc gacgggatcg accgcctaca atctctcggc cggcgggccg atcatctacc
    13021 cgacgatggg cgcgctcgtg ctgacgccca tctgcccgca cacgctctcg aaccgcccgc
    13081 tcgtcgtctc cgacgacatg gagatcgagc tgacgctcaa gaccgacaaa gaggaggtcg
    13141 cgctcacgct cgacgggcag atcggcatcc cgctcgaata cgaagaccgc gtcacggtgc
    13201 gtaagagccg cacggccttc cacttgatcc aggccgacac gcgtaactac tttgaagtcc
    13261 tgcgcggcaa actgagatgg gggaggtgaa cggaagttca aagttccgag ttcaaagttc
    13321 aaaggccgga cagcttttga ctttgaactt tgaactttga actttgaact cttatggacg
    13381 acaaagaccg cattagcgag gcggggcgca acgcgtatga gcgcgagctg acgaccgacc
    13441 tcaaggatca gacgccggat cggccgcggg ggatggcgct gcacacgcgc atcctcatcg
    13501 ggctgctggt gggcgtggtg gcgggcgtcg tcgccaaccg ggtgctcggg ggcgaccacc
    13561 ccggagtggc gtggacgatc cgcaacatca cggagccggt cggggcgctc ttcctgcggc
    13621 tgctgctgat gatcgtcgtc ccgctcgtct tctcgtcgct cgtcgtcggc gtggccggca
    13681 tcggcgacgt gcgccgattg ggccgggtgg gtctcaagtc gttcgcctac acgttcatca
    13741 tttcggccat ctcggtcgtc atcgggctga cgctggcgaa caccatccgc cccggcgagc
    13801 gcatcgacgc ggcgacctcg cagggcttgc aggcgcgcta cggcgaggac gccacgaagc
    13861 gcgtggccga cgcgacgaag accggcacga ccgactcggt gctcatgcag gtggtcaaga
    13921 ccatcgtgcc aacgaacccc gttgcggccg tggccggcat caaagcgggc gaggagcggc
    13981 agaccgacac gcccgacatg ctccacctga tgttcttcgc cgtgctcttg ggcatcgccg
    14041 cgacgatgat cccgaccgaa gtgtcggcgc cgctgctgcg cggccttcag gcggtcttcg
    14101 agatcgccgc gaagatcatc gacatcatca tgaaggtggc gccgtttgcc gtcgcctgcc
    14161 tgctcttcaa taacacggcg cgcttcggcc tcgacctcct tcaggcgctc gcgtggttcg
    14221 tcgtcaccgt gctgttgggt ctcgccatcc acatgttcgg tgtctactcg ctctcggtct
    14281 acttcctctc gcgcatcaac ccgctggagt tcttccgccg catcaagacc gtgatgctga
    14341 cggccttctc cacctcctct tccaacgcca cgctgccgac ggcgctacgc gtctcggagg
    14401 aaaacctcgg cgtgccgcgc gagatcaaca gcttcgtcct caccgtgggc gccaccgcca
    14461 accagaacgg cacggcgctc tacgagggcg tgacggtgct ctttctggcg cagctcgccg
    14521 gcgtagacct gacgctgacg cagcagctcg gcatcgccta cttcgccatc ctcggcggca
    14581 tcggcacggc gggcgtgccc tccggctcga tcccgttcat catcctcatg ctcgccacct
    14641 acaacatccc gaccccgctc atcgccatca tcctcggcgt tgaccggatt ctcgacatgt
    14701 gccgcaccac tctcaacgtc acgggcgaca tcacggcggc gacctacgtc gcccgaagcg
    14761 agggctacga acttttgaag ccgcacgtgc cggagcttca gcggcgcggc gtcgttgcgg
    14821 gcggcccgtc gagccgcgac gtgctggatt gattttacaa acccgcgtag gccgccgacc
    14881 ggaacagaca acgggaggcg gtgcgctgtt gggggacgtg agaaatggcc gaattgattc
    14941 atgagtgtga gacgaaagtg aaagatgccg gcggcgtgac ttaccgcgca gccgtctatg
    15001 ggaaggagcg cgcggacggc acgtgggagg ggtggatcga gtttcgtccg ctggctggag
    15061 acgcgctggt gttgcgtacc gggcaggaga cctcgcagcc caatcgtgac gcgctcgcct
    15121 attgggcgtc ggggctggag ccgatttaca tcgacggggc gttcagccgc gcccgttgaa
    15181 gggccgtgcg aagggagtcg ggcgatggac aagcgcaagc tcgcggagat tgaagaatac
    15241 gtgctcggcc tgttccgcag cgcggggacc ggccggctgc tcgcccgcga aattttcgac
    15301 cgctcgactg aatacgccaa cgccgacatc gtcagggcgt tcgaggattt ggagaagaga
    15361 caacgcctcc tcgtccgcca cacggaggag gggaacgact gggtgcgcct cacgcccgaa
    15421 ggcgcgcgcg tcgccggact ccccgacacg ggcgacgacg atgcatccca cccgtccgcg
    15481 ccgccgcacc cgcccagaag cgtcccctga aaagaaggca aaggtaaaaa ggcaaaaggc
    15541 aaaagtgagg aagcgcgggc ctcttcactt ttgccctttg cctttttacc tttgctctgt
    15601 agtcactcgc cgagcgcggc gtacttgaag atgcggaagc ggtagcgttg ctgggcgcgg
    15661 atgcccgcgg ccacggcgcc gtgcagggag tcgtcccact tcgcgtagcg gtcgatgaag
    15721 gcgtcggcgg cagcgcggtc gccctcgcgc tgcacttcga gcaccttccg caagagcttg
    15781 cccaccacgt cgtgatactt gccgtagtcg atgcggagct tcttcgtctg agggtcgaaa
    15841 ctgaggagcc cctgttcgag gaagaagttc cactgcatga gctgcatcgt gttgtagggc
    15901 tgctcgcggc gcgggcggtt gttctggagg acgcggctga cgccgccggc gtagaccgag
    15961 cggagttgcg cgtcggtgta atagttctgc ttgcggagcg cttcggcgac gtagagcgcg
    16021 acgaggtcgg ccttcatctc ttcgagcaga ctcgaagcgt cctgcaaagc cgtgtcgagg
    16081 tcgcggcccg ttttggtcgc gtccacgccg agatagtggc caacttcgtg ccagagcgtg
    16141 cggtagaagc cgccctcgga ggtgaggtcg tccttgtgcg cgggggcgac ggcggcctcc
    16201 cacgcattgc ctgcgccctg gaagatgtcg gggttacgca tgatgttggc gcgcaggagg
    16261 atggtgcggc cgtagcggcg cgcgagatag ttctcgttgg ggaggatggt ggcggtgttg
    16321 ccgccgcgcg cctgcccgaa gtcggcgatt acgtcgtaga cgccgacggg gatgtcctcg
    16381 cgcactttct tatggtgttc gtagggcagg gagtcttcga gcgcctggat gccgctcatc
    16441 gccttgcgca gcgcctcggt ctcggcgcgg cgcgtggcga ggagactgaa ggcgtagaag
    16501 gtcttgacgc cgtagagcgc gtcgtcgtag acctcgtagg cgccgatctg ggcgttcaga
    16561 tttttgaagc ggcccgtgat ccacgaggcg tcgcccgact cgtagtcgtc cgagagcaga
    16621 tcgcgcgcgc ggttgcgtag gtagcgggcg aactcctcgt catccttctg gacggcgtcg
    16681 gccgcttcgt ttaagagcgc gtgcgcgcgg acggtctggt cggcgtaggc gaccgagtag
    16741 gggacggcgt agagcatttt ggggtcgggc cgcgcggaca tcttttgcag ctcggcttgc
    16801 agccccggat ggagcgtcga gagcgcgggg tgttgtcgga gttcactgag gtcgcggcgc
    16861 aggctctcgg cggtggcgcg gcggacgacc gtgcggaggt cgagcagggc gtcgcgccgc
    16921 tcggggtgcg cggcgaggaa ggcgtccgtc tcctccttcg tgatgttcca cgggtagacg
    16981 ttctgccccg gaggcgtcat ctcgacgggg aggaacggct cgcgcttgtt ttcgagcgtc
    17041 gtggcaatcg gcccctgata gagacggtag agcgtgagca ggttttgggc gtcagggccg
    17101 gtgcgtcgct ggaggtcgcg ctcggcggag agggcgcgct cgtgtcgctg ctcttcgtag
    17161 aggctctgga aaatcttccc ggcttcgagc agtttggcga cggcgcgtcg ctcgccctcc
    17221 gagaggtgcg agaggtcggg cgcgagacgc accgtctgcg tcttctccag aatctcgcgg
    17281 ctccgcgcct cgggccagta acccgcggcc ggcccctccg tcccacggcg cggcgcggtc
    17341 tgcgcgaagg gttgcgccga cacgagaatg gacgacacga cagccagcaa acttttcatg
    17401 ctcacctccc gaacgggtta gaaaactttt ctaacgtgat actgcccgcg gcgccggaat
    17461 gtcaaacacg cgggcgcgca cgcgcggcgc gatgcttgag aacatcgcgg ctcgccggcg
    17521 ggtgtgagag aaatcgtttc ccgacttcgc agcgcggccc ggtgggcgta aactggcccc
    17581 gccgtccgca aattccgcga ccgccgcgcg ccagtggaga agccgcagcc gcgcgcgccg
    17641 tcctttatgg agaggtgaca ggagagatga tttttccaat tggcgatgac aacaccgacc
    17701 gcgctacgac gccgctcgtc aactacgtcc tcatcgcgat caacgtcctg gtcttcgtct
    17761 tcgcgcaggg cctcgggtcg aacgatcagt tcacatacgc ctgggccacc gtgccgcagg
    17821 agatcgtcac gaacaccgac atcgcgcaaa acgtcccgct cggcgttgac ccggcgacca
    17881 accgccccgt ctccattccc ttgcagccga tcccggtctc cgtctatttc acgctgttca
    17941 cgtcgatgtt catgcacggc ggcttcatgc acctgttcgg caacatgctc tacctctgga
    18001 ttttcggcga caacctcgaa gaccgtctgg gccacgtgcg ctatctgctc ttttatctcc
    18061 tgtgcggcgt catcgcgtcg ctctcgcacg tgttgatgaa cacgaactct ttcattccct
    18121 ctttgggcgc gtcgggcgcc atctcgggcg tgctcggcgg ctacctgctt ttgttcccgc
    18181 gccggcaggt gcgcgttatc atgctgcgga tgctgacgac cgtgccggcc tacgtcgccc
    18241 tcggcatctg gattctgttc cagttcttcg agggctacat gaaccgcggc gcggccggcg
    18301 gcggcgtcgc ctacgcggcc cacatcggcg gcttcatcgc cggcctcctg ctcatcaaga
    18361 ttttcgccat cgggcgcggc ggcggcgcgc cgagtgtcgc acgcacagtc tgactctcga
    18421 ccagcggcgg gttgtagcag cggacggatg tgttaaccct gcgtccccga cgaaagctca
    18481 ttattgtagc gattcgcaat cgcgcgcggc cacatcgacg actcgctgta gaggcgggca
    18541 atgagcgccg ggcgcatcgg ttgccggggc gcgtgcggct cggttaagat gcgcgcgtct
    18601 aagtttcaag gagcaactga gaatgtccgt gaccgacgcg acgcttcccc gtgttaacca
    18661 acagggtctc gacatccgcc cgacgccgcg cgaagagatg aacctctacc cgctcgacac
    18721 gttcccctac gaatacgcgg gccgcgagat tcacatcaag ttcgacatcc ccgagttcac
    18781 ctgcgtctgc ccgttctcgg acttccccga cttcgccacc atccgcctcg actacgtgcc
    18841 caatgaacgc tgcgtcgaac tcaagagcct caagctctac gtcaactcgt tccgcgaagt
    18901 gaaggtcttc cacgagcacg tcatcaacgt catcctcgaa gacttcgtgc gggcctgcga
    18961 tcccctcgcc gtcgaaatcg agggcgactt caacgtccgc ggcaacatca agacggtcgt
    19021 cagcgcgagc tacaagaagt caggagtcag aagtcaggag ccagaataaa agcgagcttc
    19081 gccttcctta ttctgactcc tgacttctga ctcctgactc cctgcttccc atgtctctca
    19141 gtatcatcgc catcctcgcc atcgtgctcg gcgtcgtcgc cgtggtgggg ttcttcgtgc
    19201 tcaagcgtgt gctgcgttgg gccgtgcgtc tgttcctgct gggcgtggcg gtggtgctgc
    19261 tgctggcggg cgcggtgatg tggtggtggt acgcgccggc cggcggcgac gctccggcgc
    19321 ggcagaaccg caatggcggc ggcggcgcga cgacgcgccc cgcgaaccgg cgttgaccgg
    19381 gagagtgcgc gcccctgaat gagcaagagg gaagaggact ttgccgaccc gatagaagag
    19441 gttgcgcgcg cggcgcgggc gcgtggggcg ggcgggatat ggggacgcta tcgtcgtctg
    19501 cccacgaacg tcttcgccat cagctcggtc agtctcctca acgacgcttc gagcgagatc
    19561 gtctatccct tcctctccgt tttcctgacc acgctcggag cctcggccct ctcgatggga
    19621 ctcatcgagg gcgcggccga atcggtctcc agcttcctca aactcttcgc cggctacttc
    19681 agcgaccgcc tcggcagacg caagggcctc gtcgtcttcg gctacgcgct cgccggcacg
    19741 atgcggacgc tcctcggctt cgctacctcc tggacgcacg tcctcgccat ccgcctcgcc
    19801 gaccgcgtgg gcaagggcat ccgcagcgcg ccgcgcgacg cgatgatcgc cgacgccgcc
    19861 gcgcccgacg agcgcggtct cgccttcggc ctccaccgcg cgatggatca cgccggggcc
    19921 gtcatcggcc cactcttcgg cttcgccatc atcgccctca tcgcgcaaga ccgcagcgcg
    19981 ccgagcctcg gggagttgcg cacgctcttt ctgctcgcgg ccgtgccggc gctggcggcc
    20041 gtgctcgtcg ccgccctttt cgtccgcgac gcgccccgcg aggtgaagac cgacttgcgc
    20101 gaggtgtcgc cgccgccgcg tctcagcctg cgcggattcg acacgaactt caaacgcttc
    20161 ctcgtcgtcc tcgcgctctt cacactggcg aactcctccg acctcttcgt gctccggcgc
    20221 gcgcaggagg cgggcgtcgg agccgcgacc atccccctgc tgtgggccgc gctccacgcc
    20281 tgcaaggtgc tgacttcgct cgtgggcggc gacttgtccg accgcctcgg gcgcaagcgg
    20341 atgatcgtct cggggtggat tctctacgcg tgcgtctacg cggggttcgc cttcgtgtcg
    20401 cggcccggtg aggcgtgggc gctgtttctg ctctacggcg tctacgcggg gctgaccgaa
    20461 ggggcggaga aggcgctcgt cgccgacctc gtgccggccg agcagcgcgg cacggcctac
    20521 gggctgtata atctcgcctt cagcatcacg gttttaccag catcgctttt gacgggcgcg
    20581 ctgtggaact tctacggggc gcgcacggcc ttcctcgcga gcgcggccgt cgccacgttc
    20641 gccgccgggc tgctcctgct ggcggtacgc acgtcccgct gggacgcacg ggcgccgcag
    20701 ctttgagttt tgacaaccgg gcgcgtacat tgtcccgcgc cctctcttcg cgtccgtcgc
    20761 gcctttcgcg tgaggcgtaa acattcatcc gacagcagtt cgctctcatg tcttcaaccg
    20821 actaccgcct catcaccgat atcacagaag tcagcgccgc ctgcgaggag ctttcgcgcc
    20881 acggggccgt gggcttcgac tgcgaaacca cctcgctcga cccctacggc ggccgcctgc
    20941 gtctcgtgca aattgcctcg cccgacggcg tctgcgtctt cgacctcttc gcgctcgcgc
    21001 ccgacggcca cgccgacaag gtcgaagcgt tggagccgtt gcggcggctg ctcgcggcca
    21061 cgaagccggt caagatcgcg cacaacgcga agttcgacgc caagtgggtg cgtcacgcgc
    21121 tcggcgtcga gttgcacggc gacttcgact cgcgcgccgg cgggaacggc gcgaacggcg
    21181 agggcggaac ggtcgagcgc gccgggctgt tcgacacgct gctggcgagc cagatcatct
    21241 cggccggcga gaccgaagac cggcacgggc tggcggcggt cgtcgagcgt tatctcggcg
    21301 agactgtgga caagtcgcag caggtgagca actggggcgg ggaactctcc gaggcgcaac
    21361 tccgctacgc cgcgcgcgac gccgccgtga tgctccccct gcgcgacaag ctcatcacgc
    21421 aactgcgcgc caacaacctc ggccgctgcg cccaactgga gttcgagtgc gtcgtgcccg
    21481 tggcgtcgat ggagctggcc ggcgtctacc tcgacgccgc gtgctggcgc gagcagttgg
    21541 ccgtcgtcga gaagatgcgt aagacgctcg cggaagagtt acaggacatg ctcgccgagg
    21601 agtcgccgca gcaatccttc ttcggcccca cgcgtgtcga actcaacctc gactcgcacg
    21661 tccagctcac caaggccctg acccgactcg gcgtgcccgt ccccgactcg acgcgcaact
    21721 ggaaattgca gccgctcgcc aaccagtatc cgtccgtcgc caagctgctc gaataccgca
    21781 ccgtgcagaa ggcgctcacc agctacggcg aaaacatcct caaagagatc aaccccacga
    21841 cgggccgcat ccacgccaac ttccaccaga tcggcgcgcc caccggccgt ttcgcgtgca
    21901 ccaatcccaa catccagcag gttccgcact ccatcgagta ccgccgctgc ttccgcgcgc
    21961 ccgaaggcaa gaagctaatc atcagcgact attgcgtggc cgcgggcacg cgcgtcgcta
    22021 cacgacgcgg gctggtcaaa gtcgagagcg tgcgggtagg caacgaggtc ctcgttgagg
    22081 atggaagtgc tcaacgcgtc tccgccactg tgagccgcgg gagactgccg gtcgtcaccg
    22141 tcacgctgaa gaacggctac gccttgacgg cgactgactt gcaccgcatc cgtgtgattg
    22201 acgaggaggg gcggtatgtc tggcggcgca ttggggagtt gcgacgaacc gactgcgtgg
    22261 ccgtccagcc ggggcgcggc ctccacgacg agctgtctta cgtggagttg cccgaggcag
    22321 tcagcgag
//
LOCUS       DASVES010000071         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1604078, whole genome shotgun sequence.
ACCESSION   DASVES010000071 DASVES010000000
VERSION     DASVES010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604078"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VF240_02655"
     CDS             <1..325
                     /locus_tag="VF240_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174174.1"
                     /translation="TMRFAAKVPGQATRVRDVNMDFRYGASFGVPLAEAYERLILDCI
                     LGDSTLYARGDMAERGWELVTPILEEWEVGKEDTTFPNYEAGTWGPQKSFAIIERAGR
                     RWRRP"
     gene            497..1672
                     /locus_tag="VF240_02660"
     CDS             497..1672
                     /locus_tag="VF240_02660"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX7174175.1"
                     /translation="METQRHESAVALPTAKGIDVVRLERELTAMWSKATAGEEGKEGP
                     GVMRACVLNLIVYEPLHQEREAIDALLDEVFESNPCRALIIFADANSTEERLDARVST
                     RCSQSSKGARQICGEQVTIEASGAAVARAGSAVAALLVPDVPVFLWWKDIPHYEDKLF
                     ERLVGVADRVVIDSAAFDHPREDLLRLAEVVRANPEYMAASDLCWGRLTPWRSMLASF
                     WDVKDYAPHLRALDRIEITFDSAAAAAGPKISAPALLCAGWLCSRLGWKVVRCVASGE
                     DAPAEVELSAADGRGMRLVFKHAGRGTVETSGLRSLALKSEETGAEFWVELKMGEAKL
                     ETATRLGASGRVVGRLTDFPVRSEGEWLSGELALLTRDKVYEQAVAACAEVLKSLNR"
     gene            1709..>2638
                     /gene="glk"
                     /locus_tag="VF240_02665"
     CDS             1709..>2638
                     /gene="glk"
                     /locus_tag="VF240_02665"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7174176.1"
                     /translation="MIIAGDIGGTKTNVALFEPREGGLVEPVVFDSYPSGGYDSLESI
                     LGHFISKHQPRLTHACFGVAGPVQDGFVATSNLAWDVRAQSLQDVLGLPHVKLINDLE
                     AAAYGVGCLGPSQLFTLHEGRDGVEGHRALIAAGTGLGMAGLFWDGRRHRPIPSEGGH
                     IDFAPRNEREIELLRYMKTKIGGRVSYERVLSGPGLFEIYEFLREQRFAEEEAWLAEE
                     IRGGDPSAAVSNAALGGKSGLAAEALDIFVSIYGGMAGDLVMLLKPAGGLYVGGGIAP
                     KILEKLKDGTFLRSYLDKGRMSFIVESTPVRVIL"
BASE COUNT          478 a          823 c          876 g          461 t
ORIGIN      
        1 caccatgcgc ttcgcggcga aggtgccggg tcaggccacg cgcgtgcgcg acgtgaacat
       61 ggacttccgc tacggcgcgt ccttcggcgt gccgctcgcc gaagcctacg aacgtctcat
      121 cctcgactgc atcctcggcg attccacgct ctacgcgcgc ggcgacatgg ccgagcgcgg
      181 ctgggagctt gtcacgccga ttcttgaaga gtgggaggtc ggcaaagaag acaccacgtt
      241 tcccaactac gaggccggca cgtgggggcc gcagaaatct ttcgccatca tcgagcgcgc
      301 cgggcgccgc tggcgccgac cgtgacaagt ggttgtgaac agtaccgcga gcggtagcga
      361 gcgggtgcgg ccaacatgag aggcccgccc cgcgcatcca cacccgctcg ctaccgctcg
      421 cggtactgtt ggagccgatg tgctcacgca atggcgcaaa gaaagtttta agcggagtat
      481 agtttttaga actgtgatgg aaacgcagag gcacgagagc gcagtcgcat tacccacggc
      541 gaagggcatc gacgttgtca ggctcgaaag agagctgacg gcgatgtggt cgaaggcgac
      601 cgcgggcgag gaaggcaaag aagggccggg ggtgatgcgc gcgtgcgtgc tcaatttgat
      661 tgtctacgaa cccttgcatc aggaacgtga ggcgattgat gctctattgg atgaggtctt
      721 cgagagcaac ccgtgccgcg cgctgatcat cttcgcagac gctaattcaa cggaagagag
      781 gctcgacgcg cgcgtctcca cgcgttgcag ccaatcgagc aagggggcga gacagatttg
      841 cggtgagcaa gtgaccatcg aggcgagcgg cgcagcagtc gcgcgcgcgg gaagcgccgt
      901 cgccgcgctc ctcgtccccg acgtgcccgt cttcctgtgg tggaaggaca ttccgcatta
      961 cgaggacaaa ttgttcgagc gccttgtcgg cgtcgccgac cgcgtcgtca tcgactccgc
     1021 cgccttcgac cacccgcgcg aagacctcct gcggctggcg gaagtcgtcc gcgccaaccc
     1081 cgagtacatg gccgcgagcg atctctgctg gggacgcctg acgccgtggc gctcgatgct
     1141 cgcgagcttc tgggacgtga aggactacgc gccgcacctg cgcgcgcttg accgcatcga
     1201 gatcacattc gactccgccg ccgccgccgc cgggccgaaa atctcggccc cggcacttct
     1261 atgcgccggt tggctgtgct cgcgtctggg atggaaagtc gtgcgctgtg tcgcttccgg
     1321 cgaagacgcg ccggcggaag tagagttgtc ggcggcagac ggccgcggca tgcggctggt
     1381 tttcaaacac gcgggtcggg ggactgtcga aacatccggt cttcgttcac tcgcgctcaa
     1441 gtccgaggag acgggcgctg agttttgggt cgagctgaaa atgggggagg cgaaactcga
     1501 aaccgccacg aggctcggtg cgtcggggcg tgtcgtcggc cggctcacgg atttcccagt
     1561 gcgaagcgag ggcgagtggc tgagcggaga gcttgccctg ctcactcgtg ataaagtgta
     1621 tgagcaagcc gtcgccgcgt gcgccgaagt tttgaagagt ctgaatcgat agccggacgg
     1681 acgcgtcccg gccgcacaac ccgaaacaat gattatcgct ggagacatcg gagggacgaa
     1741 gacgaacgtc gccctcttcg agccgcgcga gggcggcctc gtcgagccgg tcgtgttcga
     1801 cagctacccg agtggcggct acgactccct cgaatccatc ctcggacact tcatctccaa
     1861 acatcagccg cgcctgacgc acgcctgctt cggcgtcgcc ggccccgttc aggatggctt
     1921 cgtcgccacc tccaacctcg cctgggacgt aagggcgcag tcgttgcaag acgtgctcgg
     1981 cctcccgcac gtcaagttga ttaacgacct cgaagccgcc gcctacggag tcggttgtct
     2041 cgggccgtcg cagcttttta ctctgcacga gggccgggac ggagttgaag gacaccgggc
     2101 gctcatcgcg gccgggacgg gcctcggcat ggccggtctc ttctgggacg gacgccgcca
     2161 ccgccccatc ccctccgaag gcggacacat cgacttcgct ccccgcaacg agcgcgagat
     2221 cgaactcctc cgttacatga agacgaaaat cggcggtcgc gtcagctacg agcgcgtgct
     2281 ctcggggccg gggttgtttg aaatctacga gttcctacgg gaacaaagat tcgcggagga
     2341 ggaggcgtgg ctcgccgaag agatacgcgg cggcgacccc tcggccgcgg tctcgaatgc
     2401 cgcgctcggc ggcaagtcgg ggctggccgc cgaggcttta gacatcttcg tctcgatcta
     2461 cggcgggatg gcgggcgacc tcgtcatgct tctcaagccg gccgggggtc tctacgtcgg
     2521 gggcggcatc gcgccgaaga ttttggagaa gctaaaggac gggacgtttc ttcgctctta
     2581 cctcgacaag gggcgcatgt ccttcatcgt cgaatctacg cccgtccgcg tgatcctc
//
LOCUS       DASVES010000072         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_841178, whole genome shotgun sequence.
ACCESSION   DASVES010000072 DASVES010000000
VERSION     DASVES010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841178"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1058)
                     /locus_tag="VF240_02670"
     CDS             complement(159..1058)
                     /locus_tag="VF240_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174177.1"
                     /translation="MKRLTLSLLAAASLAAVLSADAPRAQTQTSNSTASVASRPGAGT
                     PSPEQIISAFSAKESEFRRALNEYQFKRDAVIQTIGMGGQITGEYKRVSRFVFDDAGN
                     RFEKILHFPMSTVKDVSITPEDLEDLGGVQAFALEASQIGKYNISYVGKERVDELDLY
                     IFDVAPKVMPNPKKTKERLFQGRIWVDDQDLQIVKARGKGVPETKESRYPTFETYREN
                     IDGRYWFPTYSYADDELVFDSGQVVRLRLRVRYSDFEKLRGKVRIIEEGEPGVDEPKG
                     TPVPDEPSNPTPTPTPSPAKPKP"
     gene            complement(1076..1231)
                     /locus_tag="VF240_02675"
     CDS             complement(1076..1231)
                     /locus_tag="VF240_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174178.1"
                     /translation="MKEDIWPSLHPSAFILHPCLLWQQAAARRHLNKALAEGLLRPRS
                     RRKLLNL"
     gene            complement(1246..2097)
                     /locus_tag="VF240_02680"
     CDS             complement(1246..2097)
                     /locus_tag="VF240_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX7174179.1"
                     /translation="MMTTATETRTITVAHSPDSDDAFMFYALANDLLDTGDLRFTHVL
                     RDIQTLNEEAMRGTYDVTAVSFHAYAYLSDRYALLPHGASIGDGYGPIVVARDEYRVE
                     DLSRLKVAIPGTMTSAYLALRILCPDFSYEVVPFDQIIDAVRDGRADAGLLIHEGQLF
                     YQRLGLKKVVDLGEWWKEWTGLPLPMGGNVIRRDLGPELVGRVSGLLRESIRYSLEHR
                     EDALAYAMQFARDMDPELADRFVGMWVNQLTLDYTDRGRAAVQRLLDEGAERGVIPRR
                     VRVEFVG"
     gene            complement(2181..2969)
                     /locus_tag="VF240_02685"
     CDS             complement(2181..2969)
                     /locus_tag="VF240_02685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174180.1"
                     /translation="MNASRDVSTFRPTDRFSDRVENYIKYRPGYPAGVLALMREEMAL
                     TPSSVVADIGSGTGLLTRMFLQNGNAVYGVEPNREMREAGERLLEEFPSFKSVEGTAE
                     ATNLDGASVDFVTAGQAFHWFDVPRARREFQRILRPGGRVVLVWNNRAASPTPFLADY
                     ERLLVEFGNDYQQVSAGYAHEESLRALFTGGYEQKVFDNRQLLNHESLRGRLLSCSYV
                     PLEADPRHGPMLERLREIFDAHQRDGRVSMDYDTQIFYGVISDE"
     gene            complement(2976..>3173)
                     /locus_tag="VF240_02690"
     CDS             complement(2976..>3173)
                     /locus_tag="VF240_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174181.1"
                     /translation="LHYGANDLDGTITEGGELSESYSVESGGEVKMSKQEIVQLIEDA
                     GFDPVERDTLYNRVERETVVA"
BASE COUNT          546 a          930 c         1083 g          614 t
ORIGIN      
        1 agaaaatcga cgttgattat cctcaacgtc gattttcttt cgcccctccg gggcttcgtt
       61 catctctaac cggcgcgtgt cccacggctt gcgccgtggg ctacaatctg ccgccgctcc
      121 gcggctcgcc gtccgggctg cggtcaggtt gttgcagctc agggcttggg cttcgcgggg
      181 ctcggcgtgg gcgtgggtgt cgggttcgag ggctcatccg gcacgggcgt gcccttcggc
      241 tcatcgacgc ccggctcgcc ctcctcgatg atgcgtacct tgccgcgcag cttctcgaag
      301 tcggagtatc tgacgcggag gcggaggcgg acgacctgtc cgctgtcgaa gactagctcg
      361 tcgtcggcgt aggagtaggt cgggaaccag tagcggccgt cgatgttctc gcgataggtc
      421 tcgaaggtgg ggtatctgga ctccttcgtc tcgggcacgc ccttgccgcg cgccttaacg
      481 atctggaggt cctgatcgtc aacccagatg cgcccctgaa acagacgctc cttcgtcttc
      541 ttcgggttgg gcatcacctt cggcgccacg tcgaagatgt agaggtcgag ttcgtcaacg
      601 cgctccttgc cgacgtagct gatgttgtac ttcccgatct gcgaggcttc gagggcgaag
      661 gcttggacgc cgccgaggtc ttcgaggtct tcgggcgtga tgctgacatc tttgacggtg
      721 gacatcggga aatgaagaat cttctcgaag cggttgccgg cgtcgtcgaa gacgaagcga
      781 gagacgcgct tgtactcgcc cgtgatttgc ccgcccatgc cgatagtctg gatgacggcg
      841 tcgcgcttga attggtactc gttgagcgcg cggcggaatt ccgactcctt cgcggagaag
      901 gcgctgatga tctgctcggg gctgggcgtg cccgcgccgg gacgcgacgc gacgctggcc
      961 gtcgagttcg aggtctgcgt ctgcgcgcgg ggggcgtccg cggagagcac ggccgcgagc
     1021 gaggcggcgg cgaggaggga aagagtaaga cgtttcatgt ttcttgccgg tgtccttaaa
     1081 gatttaaaag tttgcgccgc gaccgggggc ggagaagccc ttcggcgagc gccttgttca
     1141 gatgcctgcg ggcggcggcc tgttgccaga gaaggcaagg atgaaggatg aaggcggaag
     1201 gatgaagtga aggccagatg tcttctttca tccttcatcc ttgccttagc cgacgaactc
     1261 gacacggacg cgccggggga tgacgccgcg ctcggctccc tcgtcgagca gtcgctggac
     1321 ggcggcgcgg ccacggtctg tgtagtcgag cgtcaactgg ttgacccaca tcccgacgaa
     1381 gcggtcggcc agctccgggt ccatgtcgcg ggcgaactgc atggcgtagg cgagagcgtc
     1441 ctcgcggtgt tcgagcgagt agcggatcga ctcgcggagg aggccggaga ctcggccgac
     1501 cagctccggg ccgaggtcgc gccggatgac gttgccgccc atcggcagcg gcaggcccgt
     1561 ccactccttc caccactcgc cgagatcgac gaccttcttc agcccaagcc gctggtagaa
     1621 aagctgcccc tcgtggatga gcaggccggc gtccgcgcgg ccatcgcgga cggcgtcgat
     1681 gatctggtcg aagggcacga cctcgtaaga aaaatcggga cacaggatgc gcagcgcgag
     1741 gtacgcgctc gtcatcgtgc cagggatagc caccttgaga cgcgacaggt cttcgacacg
     1801 gtactcgtcg cgcgcgacga cgatggggcc gtagccgtcg ccgatggacg cgccgtgcgg
     1861 cagaagcgcg tacctgtcgg agaggtaggc gtaggcgtga aagctgacgg ccgtcacgtc
     1921 gtatgtgccg cgcatggcct cctcgttcaa ggtctggatg tcgcggagca cgtgagtgaa
     1981 gcgcaggtcg ccggtgtcga gcaggtcgtt cgccagcgcg tagaacatga aggcgtcgtc
     2041 ggagtcgggc gagtgggcga cggtgatggt gcgtgtctcg gttgctgtcg tcatcattag
     2101 agcctttgaa gaacggtgat aagtgatgag tgataagtga taagaaagaa gaaagcccgt
     2161 ctgctctttc ttatcactta tcactcatca cttatcactc cgtaaaagat ctgtgtgtcg
     2221 taatccatag agacgcggcc gtccctctgg tgtgcgtcga agatttcgcg aagacgttcg
     2281 agcatcgggc cgtggcgcgg gtctgcttcg agcggcacgt aggagcagga gaggagacgg
     2341 ccgcgcaagg attcatggtt gagtagttgg cggttgtcga agaccttctg ttcatagccg
     2401 ccggtgaaga gagcgcgcaa agactcctcg tgtgcgtagc ccgcgctgac ctgctggtaa
     2461 tcgttgccga actcgacgag cagccgctcg tagtctgcga ggaacggggt cggggaggcg
     2521 gcgcggttgt tccagacgag gacgacacgg ccgccgggcc ggaggatgcg ttggaactcg
     2581 cgccgcgcgc gtggcacgtc gaaccagtgg aacgcctgac ccgccgtgac gaaatcgacg
     2641 ctcgcgccgt cgagattggt cgcctcagcc gtgccctcga cactcttgaa gctcggaaat
     2701 tcttccagca ggcgctcgcc cgcctcgcgc atctcgcggt tcggctcgac gccgtagacc
     2761 gcgttcccgt tctgcaaaaa catgcgcgtg agcagccccg tgccggagcc gatgtcggcg
     2821 acgaccgacg acggcgtgag cgccatctct tcgcgcatga gcgcgagcac accggccggg
     2881 tagccggggc gatacttgat gtaattctcg acccggtcgg aaaacctgtc ggtgggccgg
     2941 aaggtggaaa cgtctcgcga agcgttcacc aaactttaag caactaccgt ctcgcgctcg
     3001 acgcggttgt agagcgtgtc gcgctcgacc ggatcgaagc cggcgtcttc gatgagttgg
     3061 acgatctcct gcttcgacat cttgacctcg ccgccggact cgaccgagta gctttcggaa
     3121 agctcgccgc cctcggtgat cgtgccgtcg agatcgttgg ccccgtagtg gag
//
LOCUS       DASVES010000073        10813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_917017, whole genome shotgun sequence.
ACCESSION   DASVES010000073 DASVES010000000
VERSION     DASVES010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917017"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF240_02695"
     CDS             complement(<1..237)
                     /locus_tag="VF240_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174182.1"
                     /translation="MKTAIIHHPVYEKHDTGDGHPETAARYAAVMRALRDDQQLWPRL
                     LDLEAPAAPRGDVQACHTPQHFKRVERAVSEGLGY"
     gene            496..900
                     /locus_tag="VF240_02700"
     CDS             496..900
                     /locus_tag="VF240_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174183.1"
                     /translation="MKKFLSIIICAVFLLTALVPDVFGQDRRRRSRFGRKARTAAIIA
                     GGAGVGALVGGKKGAAIGAGGAGLYAFNRRAARRNFKGRTRTIGTVASGAALGAGVGG
                     AVGGKRAATAGAAAGAGGGYLYRRSQRSRRRF"
     gene            1043..1690
                     /locus_tag="VF240_02705"
     CDS             1043..1690
                     /locus_tag="VF240_02705"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7174184.1"
                     /translation="MSHDEEQQRKSRVVIETPNERREVVQQQTIRTPEREREGYSSGM
                     IAAVALAAIAATAIVFLFVMNRSDNSNTNISVNSRGAATQPTPLVQQPPVIVQSTPLP
                     QGTPIIIPPAAAPPPVIIQQQAPAAPTTVTVPPPSSSGSGTDDASVQAKLDKAIRDDS
                     TLFDAGVTATIIGGKVTINGAVSSQALKDRVEKLVYAIKGVQSVDNQITVVARTP"
     gene            complement(1783..2238)
                     /locus_tag="VF240_02710"
     CDS             complement(1783..2238)
                     /locus_tag="VF240_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174185.1"
                     /translation="MVSVFALLLYNVVPRRRNVVACASSRVALTMRALGARKKFGWAV
                     THQGGEMISVQTRQQAQLLCGPISGHVFGIITREVFSAGVGRAASRGGASGEEPSGDV
                     SGDGGDAETLEDVARREREYLRGRVREELGREPTEAELDEWLRENTEGS"
     gene            complement(2282..4879)
                     /locus_tag="VF240_02715"
     CDS             complement(2282..4879)
                     /locus_tag="VF240_02715"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX7174186.1"
                     /translation="MSTAFRPTPQENPLALNVTGGRAAVPEQGLNARRVISKRYSLKD
                     AQGNPIEEWSDVVRRVVGHVSLAETDEQKRDEFYEALSGIMFRREFVPNTPCLVNAGK
                     PSGQLAACFVLDVPDSIAGIMKTATDAAIIHQTGGGTGFTFEKLRPAGGMVRSTHGVA
                     SGPVSFMNIFNTTTDTVKQGGVRRGANMGIMRVSHPDVLRFIHAKNDQHSLTNFNISV
                     TVTDDFFKAVNDGEWYQLTFEGHPWEEPVADPVQDGAYAIYRRPEGSTLTFRDRAAFL
                     EAKESGLLDACTREEAPRAGMVYAPDIWNRVVASAHRYAEPGVIFIDEVNRHNHMIAS
                     MGPIYATNPCAEQALHFNNSCNLGSIDLAKFYDAETRVDWDRLADATRWCTRFLDNVI
                     DVCAWPLPEIDDVVKRTRPVGLGIMGFADLCIRLEIAYGSPTSIDFMDEVMSFVRREA
                     WAESCRLGAEKGVFPEYEPNKESYDRFLRDEIGIPADVPLTPRNYETTTIAPTGTISL
                     VAETSSGVEPNFSWAYVRHDTLGKRTYVHTLAAQVLGMEVDQTDQESIDRAAEHVVAH
                     EHELPDYFISAMSITAEQHVHVLAAAQRNVDNGVSKTCNGAADDTVESVDHLYRLARE
                     LGCKAVSYYRDGSRDSQVLSAVKGKEAKGEVACDAAIIAEATVTETTAGEAPAATPET
                     DELAVRAAAAEVIKEAARIERPRELKGATWQIPFDYQNLYVTVNHDGLQILEVFATGP
                     ISGGVGLLASKMLRGGFESREVARSLNKVTGTHAVWFNERLLTSPEQAVAECIMLTAR
                     RLQDQPDSARAAATGDKSKTYTAAPTAAAHAGTNMNPLISDCPECGGQLEHASGCDSC
                     RDCGYSKCK"
     gene            5388..6308
                     /gene="hslO"
                     /locus_tag="VF240_02720"
     CDS             5388..6308
                     /gene="hslO"
                     /locus_tag="VF240_02720"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX7174187.1"
                     /translation="MERSNTDAADSLVRAAGADGQIRCMAAVTTRTVAEAARRHGVSP
                     TVAAALGRTLTGALLLGAGIKEFDRLTVQIDGDGPVGGITAESNQRGEVRGYVRHPEA
                     DAPLNAEGKLDVRGVVGGGMLYVTYESGFDIGLYREPYRGSVPLVSGEVGEDIAYYLA
                     KSEQIPSAVLLGVMVRARESGETFVEAAGGLIVQVMPGADEGVVAAIESSVLQTPHTT
                     QLIREGAQPADLLRAVLGDVPFEILGERPVSFACPCSYERAFSLISSIDRGELESMLA
                     EDRGAAMTCHFCNETYHLDEAVLAGILQAK"
     gene            complement(6365..7198)
                     /locus_tag="VF240_02725"
     CDS             complement(6365..7198)
                     /locus_tag="VF240_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174188.1"
                     /translation="MAERKFNLDYLKEALKEPVNFWGMAGFAVAAAYTQSVIPLAAAL
                     VTEGAYLATVPASNLYRRLVDRRARERQLKERNRMREKLIESFDPREREAVLYLKWLR
                     KQIFDNYQKFTGAKELPTNIKSLDQRWEDFVDLLDVYRRRKNHLRSINRQAVQNQLTQ
                     AERAVEGAHDERARRVQQANVEILRRRLAQFSELERSVKLVEGQLQSIENFFGLVNDQ
                     VVTLPTPERVSSLDFEQLSDSIAMTKQMLEETSDVIGALDTHNREIGNYDLLPSNTSA
                     K"
     gene            complement(7327..8055)
                     /locus_tag="VF240_02730"
     CDS             complement(7327..8055)
                     /locus_tag="VF240_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX7174189.1"
                     /translation="MGLWARMKRSLRAMFGGVIEKTEDPELILQQTIRDMHDRVPELN
                     NSVAQVMSTERLLARSKERLEAQVVDLDSKIKASIKMNRDDIATAYIGQLQQAQLDLQ
                     KTGQQLEYASLASKQALKARDNYVIQMKRRSAEAIQLINQSKQAKLQEQLAQTMASFQ
                     LGDDASTFNEMREKIDRRVATAEAKLELGTSSVDNQMQEIEREAMDMQLHDKLLTYKQ
                     QMGLLGSGEKETPALPAAADEKPA"
     gene            complement(8348..9667)
                     /gene="purD"
                     /locus_tag="VF240_02735"
     CDS             complement(8348..9667)
                     /gene="purD"
                     /locus_tag="VF240_02735"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX7174190.1"
                     /translation="MNILVVGSGGREHALCWALGRGAGESARRILCAPGNAGIATHAL
                     CVSVGATDVAALAALAEDERIDLTIVGGEAPLAAGLVDEFERRGLIIAGPVREAARLE
                     SSKAFAKEFMARHRIPTARHRVAESVAEAREHLRGGEFGAEDAGAVVKADGLAAGKGV
                     VVARTRAEAEAAAVEMLEGGRVGGEAARRIVIEEALEGPEASLLLWTDGRDYRLMPAA
                     RDHKRVGEGDAGPNTGGMGAVTAPEVIDAETLALCVREIVEPTLEGARADGLRFRGIL
                     FIGLMLTREGPRVLEYNVRFGDPEAQAILVRLQTDPAEIFDAVARGRLNETNVSWSEE
                     SSACVVLAARGYPERPETGARIVGLEAASSLPHVQIFHAGTAHAPGGGYQTAGGRVLG
                     VTATGPTLDAALSHCYAAAARIEWDGMHYRRDIGRFAEGKNAVNRQS"
     gene            10038..10418
                     /locus_tag="VF240_02740"
     CDS             10038..10418
                     /locus_tag="VF240_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX7174191.1"
                     /translation="MSSQVQPAEESQSEWSHAEVEFEDTTIKCVDCGEDFIWTAGEQV
                     FFHDKGLKNSPKRCKPCKQAKNERLAAIAAAQAAGVRQRIEVSVNCAQCGQQTTVPFY
                     PSQGRPVFCRSCFLASRGADGEDS"
BASE COUNT         1755 a         3492 c         3594 g         1972 t
ORIGIN      
        1 gtagccgagc ccctcgctca cggcgcgctc gaccctcttg aagtgctgcg gcgtgtggca
       61 cgcctgtacg tcgccgcgcg gcgcggccgg cgcttcgaga tcgagcaggc gcggccaaag
      121 ctgctggtcg tcgcgaagcg cccgcatcac ggccgcgtag cgcgcggccg tctccgggtg
      181 tccgtcgccc gtgtcgtgct tctcgtagac ggggtgatgt atgattgcgg tcttcatagt
      241 gccgattatg cggaagcgcc ggagacaggt caaagcaggg ccgccccgat gcttcaaatt
      301 catacgccgg cgcgcgttct gtgtcgcaaa agagcaacca cagacgacct ttccgacgac
      361 gttaaatcgc tgacctgctt gtctttcgac tctggcgacg accgcgcccc acgcggaatg
      421 gtatttgctc cgacaaacgg cgtgaatcat ctctccatta acatttttcg aggctagagg
      481 acgagggaga atgttatgaa aaagtttttg tcaatcatca tctgcgcggt gtttctgctg
      541 acggcgctcg tgcccgacgt gttcgggcag gacaggcgcc gtcgctcgcg cttcgggcgt
      601 aaggcgcgca cggcggccat catcgcgggc ggcgccggcg tcggcgcgct cgtcggcggc
      661 aagaagggcg cggccatcgg cgccggcggc gcgggcctct acgctttcaa ccgccgcgcg
      721 gcgcgacgca acttcaaggg ccgcacgcgc accatcggca ccgtcgcgag cggcgcggcg
      781 ctgggcgccg gggtcggcgg cgcggtgggt ggtaagcgcg cggcgacggc cggcgccgcg
      841 gccggcgccg ggggcggcta cctctatcgt cgcagccaga ggtcgcgccg tcgcttctaa
      901 gtttgttaca gggccggatg ggggcgacca cgaaagtggc cgcccccatc cgcttcgcac
      961 tttcgcgcat gtcatctcgg acgacacacc ctcgcgctgg gtcccatcat ttttctgact
     1021 tcaaaggacg gagaaagttt tcatgtcaca tgacgaagag cagcaacgca agagccgcgt
     1081 tgtgattgag acgcccaacg agcggcgcga ggtggtgcag cagcagacta tccgcacgcc
     1141 cgagcgtgag cgcgaggggt attcgagcgg catgatcgcg gccgtcgcgc tggcggcgat
     1201 tgccgcaacg gccatcgtct tcctgttcgt gatgaaccgc agcgacaact cgaacaccaa
     1261 catcagcgtc aactcgcgcg gcgcggccac gcagccgacg cccctcgtgc agcagccgcc
     1321 cgtcatcgtc cagtccacgc cgctgccgca gggcacgccc atcatcatcc cgccggccgc
     1381 ggcgccgccg ccggtcatca ttcaacagca ggcgccggcc gctccaacga cggtcacggt
     1441 cccgccgccc tcctcttccg gcagcgggac ggacgacgcc tcggtgcagg cgaagctcga
     1501 caaggccatc cgtgatgact ccactctctt cgacgcgggc gtgacggcca cgatcatcgg
     1561 cgggaaggtg acaatcaacg gggcggtcag ctcgcaggcg ttgaaggatc gcgtcgagaa
     1621 gctggtctac gccatcaagg gcgtgcagag cgttgacaac cagatcacgg tcgtcgcacg
     1681 cacgccctag ctatcaagta aaaagacaaa aggtaaaagg ctaaagtgaa gaggcgagac
     1741 gcttcctcac ttttgccttt taccttttgc cattctactt actcaggagc cttccgtgtt
     1801 ctcacgcagc cactcatcca attccgcctc cgtcggctcg cgcccgagtt cttcgcgcac
     1861 gcggcccctc aggtattcgc gctcgcgccg cgccacgtct tcgagcgttt ccgcgtcgcc
     1921 cccgtcgccg ctcacatcgc ccgacggctc ttcaccagac gcaccgcccc gtgacgcggc
     1981 gcggcccacg cccgccgaga aaacttcacg cgtgatgatc ccgaacacat gccctgaaat
     2041 cgggccacaa agtaactgcg cctgctgacg cgtctgcaca gaaatcattt cgcctccctg
     2101 gtgagtgacg gcccaaccga attttttgcg ggcaccgagc gcgcgcatgg taagcgcaac
     2161 gcgactgctc gcgcaagcga cgacatttcg ccgccgcggc acgacattat aaagaagaag
     2221 ggcgaacacc gaaaccaagt gttcgccctt cgcaagttgc tgccgccgcc gcgcgcagcg
     2281 cctacttaca cttcgagtag ccgcagtcgc ggcaggagtc gcagccggag gcgtgttcga
     2341 gttgtccgcc gcactcgggg caatcactga tgagcgggtt catgttcgtc cccgcgtggg
     2401 cggcggcagt gggcgccgct gtgtacgtct tcgacttgtc gcctgtggcg gcggcgcgcg
     2461 ccgagtcggg ctgatcctgg aggcggcgag ccgtgagcat gatgcactcg gcgacggcct
     2521 gctcggggct ggtcagcaga cgctcgttga accagacggc gtgcgtgccc gtgaccttgt
     2581 tgagactgcg cgcgacctca cgcgactcga acccgccgcg cagcatcttc gaggcgagta
     2641 aacccactcc gcccgagatc ggccccgtcg cgaagacttc gagaatctgt aggccgtcgt
     2701 ggttgaccgt gacataaaga ttctggtagt cgaacggaat ctgccacgtc gcgcccttca
     2761 actcgcgggg ccgctcgatg cgtgcggctt ccttgatgac ctccgcggcg gctgcgcgca
     2821 cggccaactc gtccgtctcc ggcgtggctg ccggggcctc gccggcggtc gtctcggtca
     2881 cggtcgcttc ggcgatgatg gccgcgtcgc aggccacttc acccttcgcc tccttgccct
     2941 tgaccgccga gagcacctgc gagtcgcggc tcccgtcgcg gtagtagctg acggccttgc
     3001 atcccagctc gcgcgcgagc cggtagaggt gatcgaccga ctcgaccgtg tcgtcggccg
     3061 cgccgttgca ggtcttcgag acgccgttat cgacgttgcg ctgcgcggcg gcgagcacgt
     3121 gtacgtgctg ctcggccgta atcgacatgg ccgagatgaa gtagtcgggc agttcgtgtt
     3181 cgtgcgcgac gacgtgctcg gccgcccggt cgatggactc ctgatcggtc tggtctactt
     3241 ccatgccgag cacctgcgcg gcgagcgtgt ggacgtaagt gcgcttgccc agcgtgtcgt
     3301 ggcggacgta ggcccaggag aagttcggct cgacgcccga ggaggtttcg gcgacgagcg
     3361 agatggtgcc ggtcggcgca atcgtcgtcg tctcgtagtt gcgcggggtg agcggcacgt
     3421 ccgccgggat gccaatctcg tcgcgcagga agcggtcgta agattctttg ttcggctcgt
     3481 actcggggaa gacgcccttc tccgcgccga ggcggcagct ctccgcccac gcctcgcggc
     3541 ggacgaacga catcacctcg tccatgaagt cgatggaggt gggggagccg taggcgattt
     3601 cgagccggat gcagaggtcg gcaaatccca tgatgccgag gcccacgggg cgagtgcgct
     3661 tcaccacgtc gtcgatctcg gggagcggcc acgcgcacac gtcgatcacg ttgtcgagga
     3721 agcgcgtaca ccagcgcgtg gcgtcggcca gccgatccca gtccacgcgc gtctcggcgt
     3781 cgtagaactt ggcgaggtcg atggagccga ggttgcacga gttgttgaag tgcagggcct
     3841 gctcggcgca ggggttggtg gcgtagatcg gccccatcga ggcgatcatg tggttatggc
     3901 ggttgacctc gtcgatgaag atgacgcccg gctcggcgta gcggtgcgcg gaggcgacga
     3961 cgcggttcca gatgtcgggc gcgtagacca tccccgctcg cggagcctct tcgcgcgtgc
     4021 aggcgtcgag caggcccgac tctttggctt cgaggaatgc ggcgcggtcg cggaacgtga
     4081 gggtcgagcc ttcggggcgg cggtagatgg cgtaggcgcc gtcctgcacg gggtcggcga
     4141 caggctcctc ccacgggtga ccttcaaagg tgagctgata ccactcgccg tcattcacgg
     4201 ccttgaagaa gtcgtccgtc accgtcacgg agatgttgaa gttggtcagg gagtgctggt
     4261 cgttcttggc gtggatgaac cggagcacgt ccgggtgcga gacgcgcatg atgcccatgt
     4321 tggcgccgcg gcgcacgccg ccctgcttca cggtgtcggt cgtcgtgttg aagatgttca
     4381 tgaacgagac cggccccgag gcgacgccgt gcgtggagcg caccatcccg ccggcggggc
     4441 gcaacttctc gaaggtgaag ccggtgccgc cgcccgtctg atggatgatg gccgcgtcgg
     4501 tcgccgtctt catgatgccg gcgatggagt cgggaacgtc aaggacgaag caggcggcga
     4561 gctgccccga gggcttgccg gcgttgacga ggcacggcgt gttggggacg aactcgcggc
     4621 ggaacatgat ccctgagagc gcctcgtaga actcgtcgcg cttctgctcg tcggtctcgg
     4681 ctaaggagac gtggccgacg acccggcgca caacgtcaga ccactcctcg ataggattcc
     4741 cctgcgcgtc cttcaggctg taacgcttgc tgatgacgcg gcgcgcgttc aaaccctgct
     4801 cggggacggc ggcgcgtcct cctgtgacgt tcagtgctaa gggattttcc tggggggtgg
     4861 gtctgaatgc ggtgctcatc cggttctgcc tttccgcgcg gagacgacgc gaatgctggt
     4921 ttgctccaaa aattttcgga cgcttctact gggggtagct cgcgtccgtc aggctgttgc
     4981 tcgggtactg cggctgggct atttatgagg cgagaaatcc ctcacgcggc ccccgcgcct
     5041 gacggcgcgc tcaccgcgtt tttcttctct cgctcaagcg agcggcagta tacatacccg
     5101 cttgggggct gtcaatagcc tagcacaaga tattgggttt gtctttgtta tcagtccaat
     5161 agaagggaca ttctgagaat ctaccttaac cacgtaaggt gccggaaaca gacgataaaa
     5221 ggctcctggc gggggcgatt ttcgcgctcc gaacagccct gccggagacg gcgcgtcgtg
     5281 gactcggagg cgcccgcgac gcgtgtcttc gcgggcagta cggagcgcaa ctttttcatc
     5341 ttatactcgt tccgctctac aacaatcagg caagggggtt tcacttcttg gagagatcga
     5401 acacagacgc ggccgactcg ctcgtccgcg ccgcgggcgc cgacggacag atcaggtgca
     5461 tggccgcggt aaccacgcgg acggtcgccg aggccgcccg tcgtcatggc gtttcgccca
     5521 cggtcgcggc ggcgctcgga cgcacgctga cgggcgcgct cctgctcggc gccggcatca
     5581 aggagtttga ccgcctcacc gtccagatcg acggcgacgg ccccgtgggc ggcatcaccg
     5641 cggagtcgaa ccaacgcggc gaggtgcgcg gctacgtgcg ccacccggag gcggacgcgc
     5701 cgctgaacgc tgagggcaag ctcgacgtgc gcggcgtcgt cgggggcggg atgctctacg
     5761 tgacttacga gtcgggcttc gacatcggcc tctaccgaga gccttatcgc ggctcggtgc
     5821 cgctcgtctc gggggaggtc ggcgaggaca tcgcctacta cctcgccaag tcggagcaga
     5881 ttccctcggc cgtgctgctc ggcgtgatgg tgcgcgcgcg cgaatcgggc gagacgttcg
     5941 tcgaggcagc gggcggattg atcgtgcagg tgatgccggg cgcggacgag ggagtggtcg
     6001 cggccatcga gtcttcggtg ttgcagacgc cgcacacgac gcagctcatc cgtgagggcg
     6061 cgcagcccgc agaccttttg cgcgcggtgc tcggcgacgt gccctttgaa atcctcgggg
     6121 aacgccccgt gagcttcgcc tgcccgtgct cgtatgagcg ggccttctcg ctgatctcgt
     6181 ccatcgaccg cggcgagctt gaatcaatgc tggccgaaga ccgcggcgcg gcgatgacct
     6241 gccacttttg taacgagaca tatcacctgg acgaggccgt gcttgccggg atacttcagg
     6301 cgaagtaggg atactggctg gagtgaatta tggacgtgag gccggcgcta gcgccggcct
     6361 cgtttcattt ggcggaggtg ttcgagggca ggaggtcgta gttgccgatc tcgcggttgt
     6421 gcgtgtcgag tgcgccgatg acgtcggagg tctcttcgag catctgcttg gtcatggcga
     6481 tggagtcgct gagctgctcg aagtcgagcg aggagacgcg ctcgggcgtc gggagcgtga
     6541 ccacctgatc gttgacgagg ccgaagaagt tttcgatgga ctggagctga ccctcgacga
     6601 gcttgacgga gcgctccagc tcgctgaact gcgcgaggcg gcggcggagg atttcgacgt
     6661 tggcctgctg cacgcggcgg gcgcgctcgt cgtgcgcgcc ctcgacggcg cgctcggcct
     6721 gcgtcagttg gttctgcacg gcctgccggt tgattgagcg caggtggttc ttgcggcggc
     6781 ggtaaacgtc gagaagatcg acgaagtctt cccaacgctg gtcgaggctc ttgatgttgg
     6841 tcgggagttc cttggcgccg gtgaacttct ggtagttgtc gaaaatctgc ttgcggagcc
     6901 acttcaagta aagcacggct tcgcgctcgc gcgggtcgaa gctctcgatg agcttctcgc
     6961 gcatgcggtt gcgctccttg agctggcgct cgcgggcgcg ccggtctacg aggcggcggt
     7021 agaggttgct cgccgggacg gtggcgaggt aggcgccctc cgtgacaagg gcggccgcga
     7081 gcgggatgac cgactgcgtg taggccgccg cgacggcgaa gcccgccatc ccccagaagt
     7141 tgacgggctc cttcaaagcc tccttcaggt agtcgagatt gaatttcctc tccgccataa
     7201 cgatgatgcc gcccgtcgta tgaaagcctt tcggcccgcg tcttttacta gatgcgaccg
     7261 cgccccgctt tagctgcccg gcgcgcccga agtccgaagc cggcacgccc ggcccgtgga
     7321 ggccgcttag gccggtttct cgtcggccgc cgccgggagc gcgggcgtct ccttctcgcc
     7381 gctgccgagg aggcccatct gctgcttgta ggtgagcagc ttgtcgtgga gctgcatatc
     7441 catggcctcg cgctcgatct cctgcatctg gttgtccacg gacgaggtgc caagctccag
     7501 cttggcctcg gcggtggcga cgcggcggtc gatcttctcg cgcatctcgt tgaaggtcga
     7561 ggcgtcgtcg ccgagctgga aggaggccat cgtctgcgcg agctgctcct ggagcttggc
     7621 ctgcttcgac tggttgatga gctgaatggc ctcggccgag cgccgcttca tctggatgac
     7681 gtagttgtcg cgcgccttga gggcctgctt cgaggcgagc gaggcgtatt cgagctgctg
     7741 cccggtcttc tggaggtcga gctgcgcctg ctggagctgg ccgatgtagg cggtggcgat
     7801 gtcgtcgcgg ttcatcttga tcgaggcctt gatcttcgag tcgagatcga cgacctgcgc
     7861 ttcgagccgc tccttgctcc gggcgagcag gcgctcggtg gacatcacct gcgcgaccga
     7921 gttgttgagt tccggcacgc ggtcgtgcat gtcgcggatt gtctgctgga gaatcagctc
     7981 ggggtcttcg gtcttctcga tgacgccgcc gaacatcgcg cgcaaactgc gcttcatcct
     8041 ggcccacagt cccatctctt ctcaccctcc aaccgccggc gcctcacgcg gcgcacgacg
     8101 ttttagcgtt tggcctcacc gcctcgaaaa gaccgcgact gacgccagaa tgctattacg
     8161 tcggcccgcg ccgcggggtt caaaaacgac tcgcactctt tagcgtcatg catctcggcc
     8221 cgagtatatc aggaaggcag aaaagccgta aatcgtaaat ggtaaatcgt gaatcgtggt
     8281 tgccgcacct tcggcctgag tggttaatcg aacgccgagc gaagctcaag caatttcgat
     8341 tgacgattca cgattgacga ttcacggcat ttttgccttc cgcgaagcgg ccgatgtcgc
     8401 ggcggtagtg catgccgtcc cattcgatgc gggcggcggc ggcgtagcaa tgcgagagcg
     8461 cggcgtcgag cgtcgggccg gtggccgtga cgccgaggac gcgcccgccg gccgtttggt
     8521 atccgccgcc gggcgcgtgg gcggtgccgg cgtggaagat ttggacgtgg ggcaggctcg
     8581 acgccgcttc gaggccgacg atgcgcgcgc ccgtctcggg ccgctcgggg tagccgcgcg
     8641 cggcgaggac gacgcaggcc gaagactctt cgctccaact cacgttcgtc tcattgaggc
     8701 gtccacgcgc gaccgcgtcg aaaatctccg ccgggtcggt ctggagccgg acgaggatgg
     8761 cctgcgcctc ggggtcgccg aagcggacgt tgtattcaag cacgcgcggc ccctctcggg
     8821 tcagcatcag gccgatgaag aggatgccgc ggaaccgcag tccgtccgca cgcgcgcctt
     8881 cgagcgtcgg ctcgacgatc tcgcggacgc agagtgccag cgtctccgcg tcgataacct
     8941 ccggcgcggt gacggcgccc atgccgcccg tgttcgggcc ggcgtcgccc tcaccgactc
     9001 tcttgtgatc gcgcgccgcc ggcatcagac ggtagtcgcg cccgtccgtc cagagtagca
     9061 atgaagcctc cggcccttcg agcgcctcct cgatgacgat tctccgcgcg gcctcgccac
     9121 cgacgcgccc gccctccaac atctcgacgg ccgcggcttc ggcctccgcg cgcgtgcgcg
     9181 cgacgaccac gcccttcccg gccgccagcc cgtccgcctt gacgaccgcg ccagcgtctt
     9241 ccgctccgaa ctcgccgccg cgcagatgct cgcgcgcctc cgcaaccgac tcggcgacgc
     9301 ggtggcgcgc ggtcggtatt cggtggcggg ccatgaactc tttggcgaac gccttgctcg
     9361 attcgagtcg ggccgcctcg cgcacggggc cggcgatgat gagtccgcgc cgctcgaatt
     9421 cgtccacgag gccggcggcc agcggcgctt cgccgccgac gatggtcaag tcgatgcgtt
     9481 catcttcggc gagcgcggcg agcgcggcca catccgtcgc gccgacgctc acacagagcg
     9541 cgtgcgtggc gatgccggcg ttgccgggcg cacacaagat gcggcgcgcc gactcgcccg
     9601 cgcccctgcc gagcgcccag caaagggcgt gctcacgacc gcccgaaccg acgacaagga
     9661 tgttcatgtt ctcgacaagt cttccgctct gttcctaaaa gtcttccggc ctcgcccaag
     9721 cggtttgatg aaaaagcgcg tgatgctcgc acgctccccg aaacactgtc aagacggcct
     9781 cagcgccgcg gcacgtaata cttgacacag ttttgtgaga agtataccgt gcacagtcaa
     9841 aagggagcgg cgacgctttt tcgactctgt acacccgacc cgtcgaacac tctgactgac
     9901 gcgcgtcgcc ccagcgagtc ccgacaggct tttcagtccc ctcgcccgta aacagtcaga
     9961 gacactactt tcggtacggc ctctctcacc ggagactcgt cgccgagggt tcacttcctt
    10021 tggggggttt tagaatcatg tcgagtcagg tacaaccagc cgaggagtcg cagagcgaat
    10081 ggagtcacgc cgaggtggaa ttcgaggaca cgaccatcaa atgcgttgac tgtggggaag
    10141 acttcatctg gacggccggc gagcaggtct tcttccacga caaggggttg aagaactcgc
    10201 cgaagcgttg caagccgtgc aagcaggcca agaacgagcg cctcgccgcc atcgccgccg
    10261 ctcaggccgc cggcgtgcgc cagcgcatcg aggtctcggt caactgcgcg cagtgcggtc
    10321 agcagacgac cgtgcctttc tacccgtcgc agggccgtcc cgtcttctgt cgctcgtgct
    10381 tcctcgccag ccggggcgcg gacggcgaag acagctaacc ccggcacgcc ccacacgccc
    10441 caggtccctg aacaagcaac tctccccgtt gcgcgcaggc ggtcgcgcgc ccgccagcac
    10501 gcccggtctt cagggtgcgc tccaacacaa tctaaaaaaa attccgcggc gggcgctgac
    10561 ggtgatacgt cacaggagtt tcgactttca tatcaccgcc gcgcccgccg cgtgtcgctg
    10621 cacgcgcagc gagtctcgtt cagaacaatg gggcgtgcgc ggccgctcgg ttcgcgcggc
    10681 gcgcgggaaa gcaaaagggg cttcggacga tatcgtccga agccccttaa caaagctccg
    10741 ccgccgtctg tccttccaat gaagcctacg aggttagctg acgggctgga acaaggaagt
    10801 gttccacgac ggg
//
LOCUS       DASVES010000074         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1019036, whole genome shotgun sequence.
ACCESSION   DASVES010000074 DASVES010000000
VERSION     DASVES010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019036"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VF240_02745"
     CDS             <1..934
                     /locus_tag="VF240_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7174192.1"
                     /translation="VESDPRVTTPAHLQTLARLGFTRLSLGIQDFDPRVQQAVNRVQT
                     ESQVRAVTEEARAAGFTSINYDLIYGLPFQTLSSIELTVDAVRRLRPERIAFYAYAHV
                     PWFKPGQRHFTEADLPRGDDKRALYELGRRMLEEAGYREVGMDHFALEGDLLLRASRD
                     GRMHRNFMGYTSRHVAPLIGLGVSSISDSWNAFVQNEKTVEEYQARVGRGELPFFRGH
                     QLTREDLVLRRHILNLMTRFETDWSCAENYTPHLEAVADRLAGPLGDGLARVEGESCR
                     VTDAGRPFLRNICMAFDARLAHNTDAARQFSRTV"
     gene            940..1929
                     /gene="hemH"
                     /locus_tag="VF240_02750"
     CDS             940..1929
                     /gene="hemH"
                     /locus_tag="VF240_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7174193.1"
                     /translation="MKTGIALLNFGGPWTLGEVKPFLYRLFANPSVLVGVPAPLRQLI
                     AFAIAQVKGPSSVECYRLIGGGSPQLTWTEAQAEGLSRLMSDNGKGSGDGRGAVRVEV
                     GMRSAEPSIEHALRRLKEWGAEQLVVLPLFPQFSTTTTGTCLQEVSDSLRRLRWSPRL
                     REVNNWPDNAAYTALLRRTVDEAVERAERERGDSGDPIHVLFCAHSLPLKIVERGDPY
                     PADVRRTVEAVADGLKHSWSLCYQSRNGKMAWLEPYTEDELRRLGREGVRRIVVVPVS
                     FVSDHIETLYELDMLYRDQAHNHGITHYYRTRAFNGDPEFHKVLRSVLREANV"
     gene            1926..>2719
                     /locus_tag="VF240_02755"
     CDS             1926..>2719
                     /locus_tag="VF240_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7174194.1"
                     /translation="MIGTFDTARREATVVGAGIAGMLAAYALDSKGYRVTLLEEQGRA
                     GGLIQTVHTEHGMAERAAHSLLASPAVVEFCRELGIELAEVRPDSRARFIVRDGRLRK
                     FPLGVGETVGTLGRAAFARAGADANALDLDAWGRTHLGDAAVDYLLTPFVRGIYGIQP
                     AELGVEAAFPALSVAPGRTLLGTMFGRALKRPPKNSNGASNSHTEGGAKKQGRERRRM
                     VAPRFGMGDLTGRLERRLEERLGGRFRRGVRVEEVPEGPNVILATPA"
BASE COUNT          455 a          959 c          899 g          406 t
ORIGIN      
        1 cgtagagtct gacccgcgcg tgacgacccc cgcacacctc cagacgctcg cccgcctcgg
       61 gttcacgcgc ctgagcctcg gcatacagga cttcgacccg cgcgtgcagc aggccgtcaa
      121 ccgcgtccag accgagagcc aggtgcgcgc cgtcaccgaa gaggcgcgcg ccgccggctt
      181 cacgagcatc aattacgacc tcatctacgg cctcccgttc cagaccttga gcagtattga
      241 gttgaccgtg gacgccgtcc gcaggctgcg ccccgagcgc atcgccttct acgcctacgc
      301 gcacgtcccg tggttcaagc cgggccagcg ccacttcacc gaggccgacc tcccgcgcgg
      361 cgacgacaag cgcgccctct acgagttggg ccgccggatg ctcgaagagg cgggctatcg
      421 cgaggtcggc atggatcact tcgcgttgga aggcgacttg ctcctgcggg catcgcgcga
      481 cggccgcatg caccgcaact tcatgggcta cacctcgcgc cacgtagcgc ccctcatcgg
      541 cctcggcgtc tcctccatca gcgactcgtg gaacgccttc gtgcagaacg agaagacggt
      601 cgaggaatac caggcgaggg tcgggcgggg cgagctgccc ttcttccgcg gccaccagct
      661 cacacgtgaa gacctcgtgc tgcgccgcca catcctcaac ctgatgaccc gcttcgagac
      721 cgactggtct tgcgccgaaa attacacgcc ccatctcgaa gcggtcgccg acaggctcgc
      781 agggccgctg ggcgatggcc tcgcgcgcgt ggagggcgag agctgccgcg tcaccgacgc
      841 gggccgaccc ttcctgcgca acatctgcat ggccttcgac gcccgcctgg cgcacaacac
      901 agacgccgcg cggcagttca gcaggacggt ctgaaacaca tgaagacagg catcgcactt
      961 ctcaacttcg gcggcccgtg gacgctcggc gaggtgaagc cgtttctcta ccgcctcttc
     1021 gccaacccgt cggtgctggt cggcgtgcct gcacctttgc gccagctcat cgccttcgcc
     1081 atcgcgcagg tcaaggggcc gtcttccgtc gagtgctacc gtctgatcgg cggcggctcg
     1141 ccgcaactca cttggacgga ggcgcaggcc gagggcctga gccgcctgat gagcgacaac
     1201 gggaagggaa gcggcgacgg acgcggagcg gtgcgcgtcg aagtcggcat gcgcagcgcg
     1261 gagccttcaa tcgagcatgc gctccggcgc ctgaaggagt ggggcgccga gcagttggtc
     1321 gtgctgccgc tcttcccgca gttctcgacg acgacgacgg gcacctgctt gcaggaggtg
     1381 agcgattcgc tacgccgtct gcgctggtcg ccgcgcttgc gggaggtaaa caactggccc
     1441 gacaacgcgg cttacacggc gctgctgcgc cgcaccgtgg acgaggccgt cgagcgggcc
     1501 gagcgcgaac gcggcgactc gggcgacccc atccacgtac tcttctgcgc ccactcgctg
     1561 cctttgaaga tcgtcgagcg cggcgacccg tacccggcgg acgtgcggcg cacggtcgaa
     1621 gccgtggccg acgggcttaa acattcgtgg tcgctctgct accagtcgcg caacggcaag
     1681 atggcgtggc ttgagccgta cacggaggac gaactcaggc gcctcgggcg tgagggcgtg
     1741 cggagaatcg tcgtcgtccc cgtgagcttc gtcagcgacc acatcgagac tctttacgag
     1801 ctggacatgc tctaccgaga ccaggcgcac aatcacggca tcacccacta ctaccgcacg
     1861 cgcgccttca acggcgaccc cgagttccac aaggttctgc gctcagtcct gagagaggcg
     1921 aacgtatgat cggcacattc gacacggcga ggcgcgaggc gacggtcgtc ggcgccggca
     1981 tcgccggcat gctcgcggcc tacgcgctcg acagtaaggg ctaccgcgtc acgctgctcg
     2041 aagagcaggg acgcgcgggc gggctgattc agaccgtgca caccgagcac ggcatggccg
     2101 agcgggcggc ccactcgctg ctcgcgtcgc cggcggtcgt cgagttctgc cgcgaactcg
     2161 gcatcgaact cgccgaagtc aggcccgact cgcgcgcccg cttcatcgtg cgcgacgggc
     2221 ggctcaggaa gttcccgctc ggcgtcggcg aaactgtggg cacgctcggg cgcgctgcct
     2281 tcgcacgcgc cggggcggac gccaacgccc tcgacctcga cgcgtggggg cgcacgcact
     2341 tgggcgacgc ggccgtggat tatcttctga cgcccttcgt ccgcggcatc tacggtatcc
     2401 agcccgccga actcggagtc gaggccgcct tccccgcgct ctccgtcgcg ccaggccgca
     2461 cgctcctcgg cacgatgttc ggcagggcac tcaaacgacc cccgaagaac agcaacggcg
     2521 cgtcgaacag ccacactgag ggcggcgcga agaaacaagg gagggagcgc aggcgcatgg
     2581 ttgcgccgcg cttcggcatg ggagacctga cgggacggct cgaaaggcgt ctggaggagc
     2641 gtctgggcgg gcgcttccgc cgcggtgtac gcgtcgaaga agtccccgag gggccgaacg
     2701 tcattctcgc gaccccggc
//
LOCUS       DASVES010000075         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_715239, whole genome shotgun sequence.
ACCESSION   DASVES010000075 DASVES010000000
VERSION     DASVES010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715239"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VF240_02760"
     CDS             complement(<1..1091)
                     /locus_tag="VF240_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7174195.1"
                     /translation="MESKRFRIAFSFAGEKRDFVKQVAAILAERFGEAAILYDKYHEA
                     EFARYDLGIYLPKLYGEQSDLIVPVLCPDYDAKRWTGWEWLHIYGLLTKSDGQRVMPT
                     RFEYAEVDGLSHAAAFIELDDKTPDDLARLILERLALNEGKPKNYYTKPSSTDGAARH
                     TSIPNNLPRLQPFFGREAELAQIREALDADNRTWGALIDGPGGMGKTSLAVRAAYDCP
                     PEQFKRIVFVSVKNRELDDDGVRELGVFVLPGFLEMLNELSRELKRADITKAPEDERI
                     RLLLDALRDAQALLILDNLESLPKSDRDQLFTFVKRLPPGCKAILTSRRRIGSGSEEL
                     ILQKLSREAALETLADLAGRNPLLARTSE"
     gene            1330..>2188
                     /locus_tag="VF240_02765"
     CDS             1330..>2188
                     /locus_tag="VF240_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174196.1"
                     /translation="MTIRRATGISLLLFALAASAALPGASAQKAAGGADSVNLPAERH
                     LKNVRQLTFGGENAEAYWSGDGRSLIFQSKREGRACDQIYTMRADGSEPRMVSTGNGT
                     TTCSYIFPTKKRILYASTHLAAPGCPTPPDRSRGYVWAIYPGFDIFTARPDGTQVRRL
                     TETPGYDAEATMSVDGRRIVFTSMRDGDLDIYTMDADGRNVRRLTDALGYDGGPFFSR
                     DGKHIVYRSFHPKTPEQIERYRKLLAENVIEPGNFEIWVMDADGRNKRQVTTLGAASF
                     APYFTPDGRR"
BASE COUNT          386 a          701 c          712 g          389 t
ORIGIN      
        1 tcctcgctag tcctcgcgag cagagggttg cgcccggcga ggtcggcgag ggtttcgagc
       61 gcggcttccc ggctgagttt ctggaggatg agttcctccg agccggagcc gatgcggcgg
      121 cggctggtga ggatggcctt acagcccggc ggcaggcgtt tgacgaaggt gaaaagctgg
      181 tcgcggtcgg acttggggag cgattcgagg ttgtcgagga tgagcagcgc ctgcgcgtcg
      241 cggagcgcgt cgagcagcag acggatgcgc tcgtcctccg gtgccttcgt gatgtccgca
      301 cgtttcagct cgcgcgagag ttcgttgagc atttcgagga agccgggcag gacgaagacg
      361 cccagctcgc gcacgccgtc gtcgtccagc tcgcggttct tcacggagac gaagacgatg
      421 cgcttgaact gttcgggcgg gcaatcgtag gcggcgcgga cggcgagcga ggtcttgccc
      481 atgccgcccg gcccgtcgat gagcgcgccc cacgtgcggt tgtccgcatc gagggcttcg
      541 cggatttgcg cgagttccgc ctcacggccg aagaagggct ggaggcgtgg gaggttgttg
      601 gggatggagg tgtgccgggc cgcgccgtcg gtcgaagagg gcttggtgta gtagtttttg
      661 ggcttgcctt cgttgagcgc gagacgttcg aggataagtc gggccaagtc atcgggcgtt
      721 ttgtcgtcaa gctcgatgaa cgcggcggcg tggctcaagc catcgacttc ggcatactcg
      781 aaccgagtgg gcatcacgcg ctggccgtcg cttttcgtga gcagcccgta gatgtgaagc
      841 cactcccagc ctgtccaacg cttcgcgtcg tagtctgggc aaagcaccgg aacgatgaga
      901 tcggactgct cgccgtagag cttcggcaaa tagatgccga ggtcgtaccg cgcgaactct
      961 gcctcgtgat acttgtcgta gaggattgcc gcttcgccga accgctcagc gaggatggcg
     1021 gcgacttgtt tgacgaagtc acgcttctca ccggcgaagg agaaggcgat gcggaagcgt
     1081 ttggactcca tagttcaact ctgtgagcac gcgtaagggt ttcggacgtg gacggccgac
     1141 tctgattatc cacggcgagg gcggaatgac caatattttt tggcggcggc cggagcaggt
     1201 cggactgttc tcagaacgga gattgcggcc gcccccgctg gacgaacgcg cacgggtctg
     1261 cttcaattca acggcaagga aatcatcacc aaagagcttg cggaaatctt tcacccaaca
     1321 tccgttccca tgacaatcag acgagccaca ggtatctcac ttcttctctt cgccctcgcc
     1381 gcgtcggcgg cccttcccgg cgccagcgcg cagaaggccg cgggcggcgc cgactcggtc
     1441 aaccttcccg ccgagcgtca tctgaagaac gtccgtcaac taactttcgg cggcgagaac
     1501 gccgaggcgt actggagcgg cgacggccgc tcgctcatct tccagtcgaa gcgcgagggg
     1561 cgcgcgtgcg accagattta tacgatgcgc gccgacggct cggagccgcg catggtctcc
     1621 accggcaacg gcacgaccac ctgctcttac atcttcccga caaagaagcg catcctctac
     1681 gcctccacgc acctcgccgc gcccggctgt cccacgccgc ccgaccgctc gcgcggctac
     1741 gtctgggcca tctaccccgg cttcgacatc ttcaccgcgc ggcccgacgg cacgcaggtg
     1801 cgccgcctga ctgagacgcc cggctacgac gccgaggcga ccatgtccgt ggacggccgc
     1861 cggatcgtct tcacctcgat gcgcgacggc gacctcgaca tctacacgat ggacgccgac
     1921 ggccgaaacg tccgccggct caccgatgcg ctcggctacg acggcggccc cttcttctcc
     1981 cgcgacggca aacacatcgt ctatcgctcc ttccacccga agacgcccga acaaatcgag
     2041 cgctaccgaa agctcctcgc tgagaacgtc atcgagccgg gcaactttga aatctgggtg
     2101 atggacgccg acggccgcaa caagcgacag gtcaccacac tcggcgcggc gagtttcgcg
     2161 ccctacttca cgcccgacgg ccggcgcg
//
LOCUS       DASVES010000076         7728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_28504, whole genome shotgun sequence.
ACCESSION   DASVES010000076 DASVES010000000
VERSION     DASVES010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28504"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF240_02770"
     CDS             <1..309
                     /locus_tag="VF240_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7174197.1"
                     /translation="AAGTVNFVVTEDNGQQTMGTFALGASGENFFTITAINMQRIQSV
                     FFTTDVAMTVTNVDDVAQVRIGGAQVAAVPEPTTMLLLGTGLAGIAAKVRRRRRASSE
                     "
     gene            965..1435
                     /locus_tag="VF240_02775"
     CDS             965..1435
                     /locus_tag="VF240_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174198.1"
                     /translation="MEKKQNFLWMAGLAGALCAMLFIVPQATDGQSQRGECEQNCTRQ
                     YQACRDAANANQAACKQTFDACREGCKDIRGNTNTNGNTNGNTNTNGNVNTNTNGNGN
                     ANGNTNTNTNTNTNTNTNTNTNVNTNVNTNTNTNTNVNTNSNSNSNSNSNSNTP"
     gene            complement(1536..4643)
                     /locus_tag="VF240_02780"
     CDS             complement(1536..4643)
                     /locus_tag="VF240_02780"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7174199.1"
                     /translation="MNTRDRKAGTESGKERPAAESSVVNLSDARRAAEGRASVSRAIL
                     ERDLEESREMLDRGLSGAAESRLRHAVSSARGDRNLQARARCLLSVALEMQGRYTDAL
                     EAVKQYESAEARARLDADVASGLRVQIGLSYNYTGDQPKAIALLNVALREAEEDGSDL
                     RRGEVYAALARVYRHINEYSIARDFATRALEHFRRSGDWRGLAEAYFSAGLTSTQEGS
                     FEDGLENFEQAAKIVGERPATYLLGRIYNNMAAALWFLKRPHDGVRALEKAVSFYEGT
                     EHKANAAIGYNNLGINLTLIGEWARAEKCLARALELALQLQQDKSPPAMIYDSFGELR
                     MQQGRMAEARDYLERAVALSTEDGNKWYARQALCTLGRCRLALGETELALEAGREALA
                     LAEKIGDRQALCESKLLLAEAGLAGGDAERCEALLREVTEDTSETTTDIAVTGEMQRL
                     SGLLALARHDSTLAAHHFGRANSISEMLSDRYRAARAKFLLGRAYSAAQPERAAEFYG
                     LAAHAFRELGAALDLERAEEALADASRSVSEQHEEEPSAPAQLLTLRLAEAAASRELL
                     LRELIAVVQQETRASRILIAEAGEEGATKVVNAHGYEHEEAAAVAAALGELKTDRERE
                     DFAREAGARLLMLRGSNAPPAMLLISPRDAAHTIAGAPVEPLLRVVELGLDVCALRSR
                     ARASSDAPEQDSLTGASLMPGFIHSSPAMTRLVDEVHKIRSSDVTVLVTGESGTGKEL
                     VARAIHALSSRRSKVFVPFNCTAVPKELTEGYLFGYRRGAYTGAVSDSPGVIRTAVGG
                     TLFLDEIGDLSVDVQPKLLRFLQEGEIQPLGEQRPIKVDVRIIAATNTDLERHVAEGR
                     FREDLYYRLNVIRLRVPPLRERRSEIPTIVNYYLNHYSAKFGRRDVQITPQAVDLLMV
                     SDWPGNVRQLCNEMQRVVARAEDGATIAPDNLSPELMRTSAHLSPGGATVTPISAAAA
                     DAAADASTQGLTIADAMAELERRMIAEALRKHNGNISRAARELGLTRRGLYLKLERYE
                     MSASA"
     gene            5266..7290
                     /locus_tag="VF240_02785"
     CDS             5266..7290
                     /locus_tag="VF240_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174200.1"
                     /translation="MAETVAAAGAQTKRPMSAWKRWLLEGQVKEIEGPHEPEGKHKKH
                     PWWQVMCLTGVDYFSTLGYQPGIAAVAAGALAPIATLVLVLLTLFGALPMYKRVAEAS
                     PHGDGSISMLENLLSRWKGKLFVLCLLGFAATGFIITITLSAADATAHVIENPFVHHL
                     FDAQGWNVESKAWHIGITLALVAGLGGVFLKGFKEAIGIAVVIVIVYMLLNLVIIGVS
                     FYHLALDPQQIVNWKDRLFDAEAGGFRVGGSTLMMIGISLFLFPKLALGLSGFETGVV
                     VMPLIEGDPGDSESNPQGRIRNAKKLLTAAALIMSVMLITSSFATITLIPPDEFRPAT
                     DTTEAGKASGRALAYLAHTYLGGGFGTVYDISTILILWFAGSSALAGLLNIVPRYLPR
                     YGMAPNWAKATRPLVLIFTAIAFLVTVLFRAEVEAQAGAYATGVLALMGSAAVAVTLA
                     VWRKPGLAKWAFLLISLIFGYTIIVNVIEQPSGIKIASLFIGAIILTSLVSRVWRTTE
                     LRVEQIELDETARKFIDEVKRFGEVRIVANRCDLGDVDEYRFKEMEKRQDNHIPSRDP
                     VLFFEVTPGDASEFAGTLKIRGVDVGGYRVLRAQSPAVPNAIAAFLLHLRDTTKTIPH
                     VYFGWSEGNPLMYLLRYIAFGEGDTAPVTREILRQVEEDPEKRPAVHVGG"
     regulatory      7457..7653
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT         1343 a         2501 c         2565 g         1319 t
ORIGIN      
        1 gccgccggga cggtcaattt cgtcgtcacc gaggacaacg ggcagcagac gatgggcacc
       61 ttcgcgctgg gcgcgagtgg cgagaacttc ttcaccatca cggccatcaa catgcagcgg
      121 atccaaagcg tcttcttcac gaccgacgtg gcgatgacgg tgacgaacgt tgacgacgtg
      181 gcgcaggtgc gcatcggcgg cgcgcaggtg gcggcggtcc cggagccgac gacgatgctt
      241 ttgctgggga cggggctggc gggcatcgcc gccaaggtgc gccgcaggcg gcgggccagc
      301 tctgaatgac tgagactgac ggccggcata tcgttgaccc ggcagtcccc acttttggat
      361 tgaaggcaca aattggaaag gaggggtctg tggaaacgaa gccagttgtc ttgcctctgg
      421 tcgaggacgg ttacgtattc ggcctgttcg acgaaaaagg gcgtcagatc gcctcggggt
      481 cgagggaggt gtgtgaaacg ctgtattacc tgatcacctc ctcctcactc atgaggagcc
      541 ccccgccgat ggcgcagtcg cgtgtcgatc cgagggtgcg acggcccgca cactcaacaa
      601 tgggttgaaa tcaagtcggc cgtagatgaa gggcggcggg gtgactctaa tactccgccg
      661 cccttcaaca tcaccgcacc gggacatgcc acgcaccggc ctttaagcga aacgcacaga
      721 cgagattcgt tattacctcg atattatgat tgcgaagggc gagttttgtt gggtaagcgt
      781 cgcggtgggc aggtattgta cggttgagga ttgacgcccg gcctcgtttt gtaaagaata
      841 actccgcaca tcctctcctc agtccccctg agcctgatta attccccagt ggtctacatc
      901 gtcaccacgt tcccccttca acggggcgcg cgcgcgcggg attcaccctt aggaggtaca
      961 agcagtggag aagaagcaaa atttcctctg gatggcgggg cttgcggggg cgctctgcgc
     1021 catgttgttc atcgtcccgc aggcgacgga tggtcagagt cagcgcggtg agtgcgagca
     1081 gaattgcacg cgccaatatc aggcgtgtcg tgatgctgct aacgctaacc aggcggcctg
     1141 caagcagacc ttcgacgcct gccgcgaggg ttgcaaggac atccgcggaa acaccaacac
     1201 caacggcaac accaatggca acaccaacac caacggtaac gtgaatacga ataccaacgg
     1261 gaacggtaac gccaacggga ataccaatac caacaccaac accaacacca acaccaacac
     1321 caacaccaac acgaacgtca atacgaacgt gaacacgaac acgaacacga acacgaacgt
     1381 gaacacgaac agcaacagca acagcaacag caacagcaac agcaacacgc cctagcgtaa
     1441 gggccgggca cggttcttca gacacgaaag cggcggggtc acttcttgtg ccccgccgct
     1501 ttcgtgtctg aagacttttg gctgactcgg cgacttcagg cgctggcgct catctcgtag
     1561 cgttcgagct tgaggtagag gccgcggcgg gtaaggccga gttcgcgggc ggcgcgggag
     1621 atgttgccgt tgtgcttgcg cagcgcctcg gcgatcatgc ggcgttcgag ttcggccatc
     1681 gcgtcggcga tggtgagtcc ctgtgtggag gcgtcggcgg cggcgtcggc ggcggccgcg
     1741 ctgatgggcg tgacggtggc tcctccgggg gagaggtgcg cggaggtacg catcagctcg
     1801 ggcgagagat tgtcgggcgc gatggtggcg ccgtcctcgg cccgcgccac gacgcgctgc
     1861 atctcgttgc agagctggcg gacgttgccc ggccagtccg agaccatcag gaggtctacg
     1921 gcctgcggcg tgatctgcac gtcgcggcgg ccgaacttgg ccgagtagtg gttgagatag
     1981 tagttgacga tggtcggaat ttcggagcgg cgctcgcgca aaggcggcac gcgcaggcgg
     2041 atgacgttga gacggtagta gaggtcttcg cggaaacgcc cttcggcgac gtggcgttcg
     2101 aggtcggtgt tggtggcggc gataatccgc acgtcaacct tgatggggcg ctgttcgcca
     2161 agcggctgaa tctcgccctc ctgtagaaag cgcaggagct tgggctgcac gtccacggag
     2221 aggtcgccaa tctcgtcgag gaagagcgtg ccgcccacgg ccgtgcggat gacgccgggc
     2281 gaatcggaga ccgcgccggt gtaggccccg cggcggtagc cgaacagata gccttcggtc
     2341 agctccttgg gcacggccgt gcagttgaag gggacgaaga ccttcgagcg gcgcgaggag
     2401 agcgcgtgga tggcgcgcgc cacaagctcc ttgcccgtgc ccgactcgcc cgtgacgagc
     2461 accgtcacat cagaggagcg aatcttgtgt acctcatcga cgagccgcgt catggcgggg
     2521 cttgagtgga tgaagccggg catgaggctc gcgccggtga gcgagtcctg ctcgggcgcg
     2581 tcggagctgg cgcgggcgcg tgagcgtagg gcgcacacgt cgaggccgag ttcgacgacg
     2641 cgcaggagcg gctcgacggg cgcgccggcg atggtgtgcg ccgcatcgcg cgggctaatc
     2701 agcaacatgg ccggcggcgc gttcgacccg cgcagcatca gcagacgcgc ccccgcctca
     2761 cgcgcgaagt cctcgcgctc ccggtcagtc ttcaactcgc cgagcgcggc ggccacggcg
     2821 gcggcctcct catgctcgta gccgtgggcg ttgacgacct tcgtcgcgcc ctcctcgccc
     2881 gcctcggcga tgaggatgcg ggaggcgcgc gtctcctgct ggacgacggc gatcagctcg
     2941 cgcagcaaca gctcgcgcga ggcggcggcc tcggccagtc gcaacgtgag caactgcgcg
     3001 ggggccgacg gctcctcctc gtgttgctcc gagacgctgc ggctcgcgtc ggccagagcc
     3061 tcctcggcgc gctccagatc gagtgcggcg ccaagctcgc ggaaggcgtg ggcggcgagg
     3121 ccgtagaact cggcggcgcg ctcgggctgg gcggccgagt aggcgcggcc taagagaaac
     3181 ttggcgcggg ccgcgcggta gcggtcgctg agcatctcgg agatggagtt ggcgcggccg
     3241 aagtggtgcg cggccagagt cgagtcgtgg cgcgcaagcg cgagtaggcc cgagagtcgt
     3301 tgcatctcgc ccgtcacggc gatgtcggtc gtcgtctctg aagtgtcttc cgtcacctcg
     3361 cgcagcagcg cctcacacct ctcggcgtcg ccgccggcga gacccgcctc ggcgagaagc
     3421 agcttcgact cgcacagcgc ctgccggtcg ccgatctttt cggcgagggc cagcgcctcg
     3481 cgccccgctt cgagcgcaag ctccgtctca ccgagcgcca gacggcagcg gcccagcgta
     3541 cacagagcct ggcgcgcgta ccacttgttg ccgtcctcgg tcgagagggc gacggcccgc
     3601 tccagataat cgcgcgcctc ggccatccgg ccctgctgca tccgcagctc gccgaacgag
     3661 tcgtagatca tcgcgggggg cgacttgtcc tgttgtagct ggagggcgag ttcgagcgcg
     3721 cgcgccagac acttctcggc gcgcgcccat tcgccgatga gcgtgaggtt gatgccgagg
     3781 ttgttgtagc cgatggcggc gttggccttg tgctcggtgc cctcgtagaa ggagacggct
     3841 ttctcaagag cgcgcacccc gtcgtgcggg cgcttgagga accagagcgc ggcggccatg
     3901 ttgttgtaga tacggcccag caggtacgtg gccgggcgct cgccgacgat cttcgcggcc
     3961 tgctcgaagt tctccagccc gtcctcgaag ctcccctcct gcgtcgaagt cagccccgcg
     4021 gagaagtagg cttcggcaag accccgccag tcgccggagc ggcggaagtg ttcgagcgcg
     4081 cgggtggcga agtcgcgggc gatggaatac tcgttgatgt gacggtagac gcgggcgagc
     4141 gccgcgtaga cctcgccgcg ccgcaagtcc gagccgtctt cttcggcctc gcgcaacgcc
     4201 acgttgagca gcgcgatggc cttcggctgg tcgccggtgt agttgtagga caggccgatc
     4261 tggacgcgca ggcccgaggc cacgtccgcg tcgagacgcg cgcgcgcctc cgccgactca
     4321 tactgcttga cggcttcgag ggcgtcggtg tagcggccct gcatctccag cgccaccgag
     4381 agcaggcagc gggcgcgggc ctgtaggttg cggtcgccgc gcgccgacga gacggcgtgg
     4441 cgcaggcgcg actcggccgc gcccgacagc ccgcggtcga gcatctcgcg cgactcttca
     4501 aggtcgcgtt cgaggatggc gcgcgaaacg gaggcgcgcc cctcggccgc gcgtcgcgcg
     4561 tcggagaggt tgacgacgct cgactccgcg gccgggcgct ccttgcccga ctccgtgccg
     4621 gctttgcggt cgcgtgtgtt catttacact aaaaacaagc tcgctcgcac agtgaggcct
     4681 ggcttgcgta ctcgtttcgg atactgaggg aaatggcttg attgaaaggc gcggcggcca
     4741 atgtttatct tcgagtgcac ccttcagaac actgccaaag gcctgtagat tccagacttt
     4801 agcacacccg tgagcgctca gcaataataa gctctcagct attggctctt agctatcagc
     4861 cagaagttgt ccgtgtcagt cgtccgcggt cacttgtccg ttgtacgcgt cgccctgctt
     4921 tagcctttcc gatgtcggcg acgaatagcc cgccacaaac aactgaccac tgacaatgga
     4981 cgactgacaa agaacagcac ttagctaaaa gctaacggct aaccgccaat agctttttcg
     5041 gtattgtttt ctgcggggcg gtttggtaca aagcacacac gccaaaagct cggaagcgtg
     5101 ccgggaaggt acagcgggcg gccccgcgca tgcgcgcgcc gtaacgcccg cacaaatttg
     5161 tccccgcgcc gcgtgacgcg tgaagtcgcg cgcgcgcgcg ccggacacga acgtgggtga
     5221 acttcgccga ggctgctctc ccgcctccag tgaggggtat tgacgatggc cgagacggta
     5281 gccgccgccg gggcgcagac aaagcgtccg atgagcgcct ggaaacgctg gctcctcgaa
     5341 gggcaagtta aggaaatcga agggccgcac gagccggaag gcaaacacaa gaagcacccg
     5401 tggtggcagg tgatgtgtct gacgggcgtc gattatttct cgacgctcgg ctaccagccc
     5461 ggcatcgcgg ccgtcgccgc cggggcgctc gcgcccatcg ccacactggt gctcgtcctg
     5521 ctcacgctct tcggggcgct gcccatgtac aagcgcgtgg ccgaggccag cccgcacggc
     5581 gacggctcga tctcgatgct ggagaacctg ctctcgcgct ggaagggcaa gctcttcgtc
     5641 ctctgcctgc tcggcttcgc cgccaccggc ttcatcatca ccatcacgct ctcggcggcc
     5701 gacgccaccg cccacgtcat cgagaaccca ttcgtgcatc acctcttcga cgcgcagggg
     5761 tggaacgtcg agagcaaagc ctggcacatc ggcatcacgc tggcgctcgt ggcggggctc
     5821 ggcggggtct tcctcaaggg cttcaaggag gccatcggca tcgccgtcgt catcgtcatc
     5881 gtctacatgt tgctcaacct cgtcatcatc ggggtgagtt tctatcacct cgccctcgac
     5941 ccgcagcaga tcgtcaactg gaaggacagg ctcttcgacg ccgaggcggg cggcttccgc
     6001 gtgggcggga gcacgctgat gatgatcggc atctcgctct tcctcttccc gaaactcgcg
     6061 ctcgggcttt cgggcttcga gacgggcgtc gtcgtcatgc cgctcatcga gggcgacccc
     6121 ggcgactcgg agtcgaaccc gcaggggcgc atccgcaacg cgaagaaact gctgacggcc
     6181 gccgccctca tcatgagcgt gatgctgatt accagcagct tcgccaccat cacgctcatc
     6241 ccgcccgacg agtttcgccc cgccaccgac acgacggagg ccggcaaggc gagcgggcgc
     6301 gcgctcgctt atctggcgca cacctatctc ggcggcggct tcggcaccgt ctacgacatc
     6361 agcaccatcc tcatcctgtg gttcgccggc tcgtcggcgc tcgccggcct cctcaacatc
     6421 gtgccgcgct acctgccgcg ctacggcatg gccccgaact gggcgaaggc cacccgcccg
     6481 ctcgtgctca tcttcacggc catcgccttc ctcgtcaccg tcctcttcag ggccgaggtc
     6541 gaggcgcagg cgggcgcata cgccactggc gtgctcgcgc tgatggggtc ggcggccgtc
     6601 gccgtcacgc tcgccgtgtg gcgcaagccc ggtctggcga agtgggcatt ccttttgatc
     6661 tcgctcatct tcggctacac catcatcgtc aacgtcatcg aacagccgtc gggcatcaag
     6721 atcgcctcgc tcttcatcgg cgccatcatc ctcacctcgc tcgtctcgcg cgtgtggcgg
     6781 acgaccgagc tgcgcgtcga acagatcgag cttgacgaga cggcgcgcaa gttcatcgac
     6841 gaggtcaagc gcttcggcga ggtgcgtatc gtcgccaacc gctgcgacct cggcgacgtg
     6901 gacgagtacc gcttcaagga gatggagaag cggcaggaca accacatccc ttcgcgcgac
     6961 cccgtcctct tcttcgaggt cacgcccggc gacgcctcgg agttcgccgg cacactcaaa
     7021 atccgcggcg tcgatgtcgg cggctaccgc gtgctgcgcg cgcaaagccc cgccgtcccc
     7081 aacgccatcg ccgccttcct cctccacctg cgcgacacga cgaagaccat tccgcacgtc
     7141 tacttcggct ggagcgaggg caacccgctc atgtacctgc tgcgctatat cgccttcggc
     7201 gagggcgaca ccgcgcccgt cacgcgcgag attctgcggc aggttgaaga agacccggag
     7261 aagcgcccgg ccgtccacgt cggcgggtga gcgggattga ctcaatgatg gcaggccgct
     7321 acgcacagta ccgcgagcgg tagcgagcgg gtacgactgt gacgagcgac ttgcctggcg
     7381 cacgcgagac ccgctcgcgg ttgtggttga tttccggtgg cggtgggcgt aagattcgcg
     7441 ccggtcgttg gtgttgacaa tttgtctgcc gaagtcgccc gcggcgctcg ctatcgagcg
     7501 cgggacgggc gacgcggggg aaccaactgt cccgtcgtcc gcggcggggc cggggcgaaa
     7561 cgtcggggcg ggccaaaggg gccgccgccc gtcgtggaac acttccttgt tccagcccgt
     7621 cagctaacct cgtaggcttc attggaagga cagacggcgg cggagctttg ttaaggggct
     7681 tcggacgata tcgtccgaag ccccttttgc tttcccgcgc gccgcgcg
//
LOCUS       DASVES010000077        12391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1559481, whole genome shotgun sequence.
ACCESSION   DASVES010000077 DASVES010000000
VERSION     DASVES010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559481"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF240_02790"
     CDS             complement(<1..218)
                     /locus_tag="VF240_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174201.1"
                     /translation="MKEIPALQHPVAAAADEQQAPRGGELAGTRRLVSVKERVIEWML
                     LAAAFSSVAITVGIVGTLLYESASFFAHV"
     gene            complement(311..1375)
                     /locus_tag="VF240_02795"
     CDS             complement(311..1375)
                     /locus_tag="VF240_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7174202.1"
                     /translation="MSLSKQLTRALVALSLVSALLLSMACGGAPAGNNAGAGGAAGGG
                     EQVIKVDGSSTVFPVTEAVAEEFQREKGGRVKVTVGISGTGGGFKKFARGETDVSNAS
                     RPISKEEMEAAKQNGIEYIELPIAFDALTVVVNPQNDWVTSMTVDELKKVWEPEAQEK
                     VTRWNQIRPEWPNEKIALFGAGADSGTFDYFTEAIVGKAKSSRGDYTASEDDNVLVQG
                     IEGNKYALGYIPYAYFAPHSARMKAVGIEWSKNKTQGPVMPSPENVLSGAYNPLSRPL
                     FIYVSTKGLAKPEVKEFVEFYLKNVGALAREVKYLPLPEGAYQTATERLRNNKTGTGF
                     GGAPEIGLPVEEILKREPKS"
     gene            complement(1543..1926)
                     /locus_tag="VF240_02800"
     CDS             complement(1543..1926)
                     /locus_tag="VF240_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7174203.1"
                     /translation="MSEVIQSGADQSLATRHSSLNFYDDGHLRVEHDNYYVSLGGRAL
                     KLPRKEFLIISRLARNPERIVASEELWRHAWGARAPFNAESLHVHIYRLRRRLAPHHI
                     QIDTMVNVGYRLLAGGAQDAAGVGR"
     gene            complement(2206..6159)
                     /locus_tag="VF240_02805"
     CDS             complement(2206..6159)
                     /locus_tag="VF240_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7174204.1"
                     /translation="MREINPNVSRSGRAVAAAGVCLLLLFALALVAATAPARAQSGPM
                     GTLTGAVTDPNGAALPGVQVEARNTGTGQTRAATTNDEGRWTMPGLGVGVYEITYTLA
                     GFKPLKRGDAAVEAAVPRTFEDKLEIGDITGESVTVTGGEVLATPETATIARQITAEQ
                     LVQVPTSTRSFTQLLNTEAGVSTDLSPVSTNGNGNQSPSVNGTRTTSTSLFFNGVDAT
                     NITTNEGSLNDNIAPAPETLQEVKLQTSLYDASTGRSGGGNFQLVTKSGANDLTGSLY
                     YYLQNEKFNANDFIFNKEGIDRPVARRNEGGFTIGGPVVREKWFFFGGYQYTKAVTGF
                     VPTARSTTVLPVALRRITGERTAEALASAFNAENGCLGSNCLTAADISPVALRLFNLR
                     NPVTGDFVIPTPREGGRLIGIDRAGARAFQFGAGPVTSRTLRENNPLVQQLNVVPSEF
                     EQHQFTTRLDGRLSPNNTLNGTFFFSNFPALDSFPDPSSLASPFTLRRADRNRTLAVS
                     DQHIFGPTLINEARFGYFLLNNTRALDTPFLTDEFTSEAVGIANPALLFDDSPGTRRL
                     GHFIGRPGTNLSQFSFGGPNDSFNQRKQQTFSVADNLTWIKSNHTLRTGGDYKSHRYD
                     SSLPEEQATEFEKFDSFTQLLTGNATEADTQFGVTEKSFRFQDFSGYITDDWKVSRGL
                     SLNLGLRYELFMWPTERQGRIGNFDFSTFEQCFTQAGGPVSLCDSPSPGFIVPDNVQS
                     TGLANVDGAVAVTERAGNNHTLRGQDTNNFAPRVGFAYSPWESNRLVVRGGFGVFFDR
                     PSAAFINTIFSNYPFLREVEITVPSGNVPIADAFSAQNTTLPLSNWLPFRIVRASGTG
                     GAYVIRDNTGVARDPRGNVSSAANSGNIAETFEFRAVDRDLKTPYVQQWNLGVQYEVR
                     KDLLFEARYVGTRGRNLLQAVAFNQGYDLNDLSTPDHIYERFNQAYSAAGSPNGPLGA
                     GASARERGIGRAFGFANPFRVGGSAGCAGNVLSGVAGAPLDLNLANPITCANGVLGGG
                     QLINFEARVPVLGFNVPEALLLQSNGNSVYHGAQFSLTKRLSRGLQFNAAYTFSKSID
                     TSSTDPGSTAGSGKPDVPNTGFVVQGDQRDLEGSRAVSDFDRTHRFSLNFVYQLPTFG
                     FTSRWATGWQLSGFFQTQTGTPYSIFAPEPELQTAAQYADLVRGSGGLYRLGFGRPDL
                     CAGASLEDLRRQGADITEDAFDGSTLCSPLGQNGAIGRNVLRAPSQSRFDLGVVKNTK
                     LTEDISLEFGWDIFNVFNRANFSAPDSELGSPDFGRITNTTGGPRVMQFRTKLRF"
     gene            complement(6412..7503)
                     /locus_tag="VF240_02810"
     CDS             complement(6412..7503)
                     /locus_tag="VF240_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7174205.1"
                     /translation="MLYDTTPEKAGARRRDLVTLIGAGLLFVPSPLTVKALTEEWMKQ
                     HRRKGLAVTTIDNYRDTLNSCVLPYVGHLQIKDFQPGALERMFNGLQDRGLSDSTIKH
                     VRRVCKLFIKYAVWKKYLRESPLEGLELPKGAEPRETRSLTPAEARTLVETALLDLGD
                     LIFVFALFMGMRPKECLGLPLSNVELVDDAAALVRVTQQAVKLRRAPGHIFTRPKTRK
                     GVREVPFPAWLYRELMRLQCANAARERLLGAGWTDYGLVFPSRTGRPISEGWLRDMFA
                     RLLKRSGLPPHFTPYSLRYTYNTLLSEGGVADKARCVLMGHAREDFNRAVYVRTLPQM
                     FEGVAETLERQIFGASRPSFAPGESGPVM"
     gene            complement(7591..8310)
                     /locus_tag="VF240_02815"
     CDS             complement(7591..8310)
                     /locus_tag="VF240_02815"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174206.1"
                     /translation="MSVTEIAPDVYRISTYVPEIDMQFNQFLVRDDEPLLFHTGHRAM
                     FPFVRDAVASLIDPSRIRWLGFSHFEADECGALNEWLQQAPAAQPVCSMVGALVSVND
                     FAVRPAKGMSDGEILHTGKYRFRLLHTPHVPHCWEAGLLFEEENKTLLCSDLFHQNGN
                     VAPLTESDVIEAVRKTLVDYQAGPFANYMPYTQHTDQTLHSLADLKPKTIAAMHGSAF
                     TGDGERALRDLAIVMREVLLS"
     gene            complement(8554..8937)
                     /locus_tag="VF240_02820"
     CDS             complement(8554..8937)
                     /locus_tag="VF240_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174207.1"
                     /translation="MAGKPLGDYIQRGASGPDEVIDVHGNQVALLERSRCFQASTMSC
                     STCHDVHLPQRDAAAFSRSCLTCHKVESCGIYRERGHKIAENCINCHMPKVTSKSVVS
                     TNEGRMVQPQVRTHWIKVYPAGNNP"
     gene            complement(10158..12080)
                     /locus_tag="VF240_02825"
     CDS             complement(10158..12080)
                     /locus_tag="VF240_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7174208.1"
                     /translation="MIEVVSRSCLVLIIICVSQFPVRAQATSGPSPKSRVISEVNILT
                     SGDKAPAGVAPVRGHAHEEASKASYDLGVRYGLAGRYKEAMEAFREAILYKPTYADAH
                     FGLGHAYYDAGRFKEAAEAFMQAVALNPKDFDSIVYLGASRFRQGLYEQAVEAFKRAI
                     LIKPKSADVRYNIANAFHRMGRYEPAATYYADIKSLRPATAELYNDLGAAYGESGKYE
                     KALEALRLATDNDSDDAYAHNNLGIAYYLVGRYADAAKALERAVRLAPNDPVIQSNLR
                     QTSFNGGGATGEHVALVNTGVVGTDSKGLRWLSRSDVVRGLVGEDNRYASTDPPAAEA
                     KNNSAPPHKGVTPEVEPAVTNAPTAKVVPLRTDTAHAEKKVAPPPSDLYRLGVGDVLD
                     IRLLNERTDKSTLYRVLPGGLLEYPLFSEPLSVEGQTVEEAAERIAQELRRRAIEDDP
                     RVLVNVREYVSHAVIVSGLVAEPGTKILKREEVPLYVLIADAQPLPEAGRVLVTSHAT
                     GLRTEVALEDQAAMAVPVRRGDVLMVTKMSQQFYYVSGGVVSPGQKEFHPEITLTQAI
                     IAAGGELHRLRTVTLWSNRNVPQTGVIAPPEDKGAQSPKGGGLLTSTTYNMRDILSGK
                     VPDPRITPGDRVEARR"
BASE COUNT         2146 a         3740 c         4169 g         2336 t
ORIGIN      
        1 acgtgcgcga agaagctggc ggactcgtac agaagtgtgc cgacgatgcc gaccgtgatg
       61 gccaccgacg agaaggccgc cgccagcagc atccattcga tgacgcgctc cttcacggag
      121 acgagccgcc gcgtccccgc aagctcgccg ccgcgcgggg cctgttgctc gtcggcggcc
      181 gccgcgaccg ggtgctggag ggcgggaatc tctttcatgg tgagcagtga gcagtaagct
      241 gtgagcagta aaaacaggtt cggcggtgag cagcgagctt cgcggggcgc ctactgctta
      301 ctgctcactc ctatgacttg ggttcgcgct tgagaatctc ctcgacgggg aggccgatct
      361 cgggagcgcc gccgaagccc gtgcccgtct tgttgtttcg cagacgctcg gtcgccgtct
      421 ggtaggcgcc ctcgggcagc gggagatatt tgacttccct ggccagcgcg ccgacgttct
      481 tcagatagaa ctcgacgaac tctttgacct cgggcttggc cagccccttg gtgctgacgt
      541 agatgaagag cgggcgcgag agtgggttgt aggcgccgga gagtacgttc tcggggctgg
      601 gcatcaccgg cccctgcgtc ttgttcttgc tccactcgat gccgacggcc ttcatgcgcg
      661 cgctgtgcgg cgcgaagtag gcgtagggga tgtagccgag cgcgtacttg ttgccctcga
      721 tgccctgcac taagacgtta tcgtcctcgc tcgccgtgta gtcgccgcgg gaggatttgg
      781 ccttgccgac gatggcctcg gtgaagtagt cgaacgtgcc ggagtcggcg cccgcgccga
      841 agagggcgat cttttcgttg ggccattcgg ggcgaatctg gttccagcgc gtgaccttct
      901 cctgcgcttc cggctcccag actttcttca gctcatcgac ggtcatcgac gtgacccagt
      961 cgttctgcgg attgacgacg accgtcagcg cgtcgaaggc gatgggcaac tcgatgtact
     1021 cgatgccgtt ctgtttggcg gcctccatct cctctttcga gatgggccgg gaggcgttgc
     1081 ttacatccgt ctcgccgcgc gcgaacttct tgaagccgcc gcccgtcccc gagatgccga
     1141 ccgtcacctt gacccggccg cccttctccc tctgaaactc ctcggccacc gcctccgtca
     1201 cggggaagac cgtgctcgac ccatcgacct tgatgacctg ctcgccaccg cccgccgccc
     1261 cgccggcccc ggcgttgttg ccggccggcg cgccgccgca ggccattgag agtagaagcg
     1321 ccgagacaag cgagagcgcg accaaggcgc gcgtgagttg tttcgagaga gacatgaaaa
     1381 gctcctttga ggccgcgagt ttgatggaga ctcaaaacag gaaagatact tcggccgtgt
     1441 taaatccgtc tgaagtgagt ctgaaatcta tgtgaacaga acgcgccgcg cccggcctcg
     1501 ggccggcggc gggcgactgg taagtcggtg ccagtcatgc tcttagcgcc caacgccggc
     1561 ggcgtcttgc gcgccaccgg cgagcagtcg gtagccgacg ttgaccatcg tgtcgatctg
     1621 tatgtgatgg ggcgcgaggc gtcggcgcag gcgatagatg tgaacgtgca ggctctcggc
     1681 gttgaagggc gcgcgagcgc cccacgcgtg ccgccacagc tcttcggacg ccacgatgcg
     1741 ctccgggttg cgggcgaggc gcgagatgat gaggaactcc ttgcgcggga gtttcaacgc
     1801 gcgcccgccc agactcacgt agtagttgtc gtgctcgacg cgcaggtggc cgtcgtcgta
     1861 gaagttgagt gacgagtgac gagtggcgag tgactggtcg gcgcctgact gaatcacttc
     1921 cgacattcct tcccccattg gcccacgtta agagtgagtg acagcgagcc gctgtaagcg
     1981 gcggcagaat ctagcccacg gcgtgagccg tgggtatgcg gcaaagaaat aatcggaagc
     2041 cccggagggg cgaaagaacc ttgtgttgag cgtaatgcat ctagttgttc tttcgcccct
     2101 ccggggcttt gcattttttc caccgaccat tcccacggct cacgccgtgg gctaaagtct
     2161 tccgcgcctc cggcgctcac atcatcccgc cacccatcgc ggcttttaga atcgcaactt
     2221 agtgcggaac tgcatgacgc gcgggccgcc cgtcgtgttg gtgatgcgcc cgaagtcggg
     2281 gctgcccagc tccgagtcgg gggccgagaa gtttgcccgg ttgaagacgt tgaagatgtc
     2341 ccagccgaac tccagtgaga tgtcctcggt gagcttcgtg ttcttgacga cgccgaggtc
     2401 gaagcggctc tgcgagggcg cgcgcaggac gttgcggccg attgcgccat tctggccgag
     2461 cggcgagcag agagtggagc cgtcgaaggc gtcctcggtg atgtcggcgc cctgccgccg
     2521 caggtcttcg agcgacgcgc cggcgcacaa gtcggggcgg ccgaagccga ggcggtagag
     2581 tccgccggag ccgcgcacga ggtcggcgta ctgcgccgcg gtttgcagct ccggctcggg
     2641 ggcgaagatg ctgtagggtg tgccggtctg cgtctggaag aagccggaga gctgccagcc
     2701 cgtggcccag cggctggtga agccgaaggt cgggagctgg tagacgaagt tgaggctgaa
     2761 gcggtgcgtg cggtcgaagt cggagacggc gcggctgcct tcgaggtcgc gctggtcgcc
     2821 ctgcacgacg aagccggtgt tgggcacgtc gggcttgccg gagccggcgg tcgagccggg
     2881 gtcggtcgag ctggtgtcga tggacttcga gaaggtgtag gcggcgttga actggagacc
     2941 gcgcgacaga cgcttggtca ggctgaactg cgcgccgtgg tagacggagt tgccgttgga
     3001 ctggagcagc agcgcctcgg gcacgttgaa gccgagcacg gggacgcgcg cctcgaagtt
     3061 gatgagctgt ccgccgccga gcacaccatt cgcgcaggta atcgggttgg cgaggttgag
     3121 gtcgaggggc gcgcccgcca caccgctcag gacgttgccc gcgcagccgg cactgcctcc
     3181 gacgcggaac gggttggcga agccgaacgc gcgaccaatg ccgcgctcac gcgccgacgc
     3241 gcccgcgccc agcgggccgt tgggcgagcc ggcggccgag tacgcctgat tgaaacgctc
     3301 gtagatgtga tcgggcgtcg agaggtcgtt gaggtcatag ccctgattga aggcgacggc
     3361 ctgaaggaga ttgcgtccgc gcgtgccgac gtagcgcgcc tcgaacagta aatctttccg
     3421 cacttcgtac tgcacaccga ggttccactg ctggacgtaa ggcgtcttga ggtcgcggtc
     3481 aacggcgcgg aactcgaacg tctcggcgat gttgccggag ttggcggcgc tgctcacgtt
     3541 gccgcgcggg tcgcgcgcca cacccgtgtt gtcgcggatg acataggcgc cgcccgtgcc
     3601 ggaggcgcgc acgatgcgga acgggagcca gttactcagg ggcagcgtcg tgttttgagc
     3661 ggagaaggcg tcggcgatgg gcacgttgcc cgaggggacg gtgatctcga cttcgcgaag
     3721 gaaggggtag ttgctgaaga tagtgttgat aaaggccgcg gagggacggt cgaagaagac
     3781 tccgaagccg ccgcggacga ccaagcggtt cgactcccaa ggcgagtagg cgaagccgac
     3841 gcgcggggcg aagttgttcg tgtcctgccc gcggagcgtg tgattgttgc cggcgcgctc
     3901 cgtcacggcg acggctccgt caacgttggc gagtccggtg gactggacgt tgtcgggcac
     3961 gatgaagccg ggcgaggggc tgtcgcacag cgagacgggg ccgcccgcct gcgtgaagca
     4021 ctgctcgaag gtggagaagt cgaagttgcc gatgcggccc tgtcgctcgg tcggccacat
     4081 gaagagttcg tagcggaggc cgaggtttaa ggagagaccg cgcgagacct tccagtcgtc
     4141 ggtgatgtag ccgctgaagt cctggaagcg gaaagatttt tcggtgacgc cgaactgcgt
     4201 gtcggcctcc gtggcgttgc cggtcaggag ttgcgtgaag ctgtcgaact tctcgaactc
     4261 ggtggcctgc tcttcgggga ggctggagtc gtagcgatgg ctcttgtagt cgccgcccgt
     4321 gcggagcgtg tggttgctct tgatccacgt caggttgtcg gcgacgctga aggtctgctg
     4381 cttgcgttgg ttgaaggagt cgttggggcc gccgaaggag aactgcgaga ggttggtgcc
     4441 ggggcgtccg atgaagtggc cgaggcggcg cgtgccgggg ctgtcgtcga acaggagcgc
     4501 cgggttcgcg atgccgacgg cctcgctggt gaactcgtcg gtgaggaagg gcgtgtcgag
     4561 cgcgcgcgtg ttgttgagga ggaagtagcc gaagcgcgct tcgttgatga gcgtggggcc
     4621 gaagatgtgc tggtcggaga cggcgagcgt gcggttgcgg tcggcgcgtc gcagcgtgaa
     4681 cggcgaggcg agcgacgacg ggtcggggaa ggagtcgagc gccgggaagt tgctgaagaa
     4741 gaaggtgccg ttcaaagtgt tgttcgggct gagccggccg tcgagacgcg tggtgaactg
     4801 gtgctgctcg aactccgaag ggacgacgtt gagctgctgg acgagcgggt tgttctcgcg
     4861 caaggtgcgc gaggtaacgg ggccggcgcc gaactggaag gcgcgcgcgc cggcgcggtc
     4921 aatgccgatg aggcggcccc cctcgcgcgg cgtggggatg acgaagtcgc cggtgacggg
     4981 gttacgcagg ttgaagagtc ggagcgccac gggcgagatg tcggcggccg tcaggcagtt
     5041 cgacccgagg cagccgttct cggcgttgaa ggcggaagcc agcgcctcgg ccgtgcgctc
     5101 gcccgtgatg cggcgcaagg cgacgggcag caccgtggtg ctgcgcgccg tggggacgaa
     5161 gccggtgacg gccttcgtgt actggtagcc gccgaagaag aaccacttct cacggactac
     5221 ggggccgccg atggtgaagc cgccctcgtt gcggcgggcc acggggcggt cgatgccctc
     5281 cttgttgaag atgaagtcgt tggcgttgaa cttctcgttc tggaggtagt agtagaggga
     5341 gccggtaagg tcgttagcgc ccgacttggt gacgagctgg aagttgccgc cgcccgagcg
     5401 acccgtcgag gcgtcgtaga gcgaggtctg gagcttgacc tcctgaagcg tctcgggcgc
     5461 gggcgcgatg ttgtcgttca gcgacccttc gttggtggtg atgttagtcg catcgacgcc
     5521 gttaaagaag agactcgtcg aggtggtgcg cgtgccgttg accgaaggcg actggttgcc
     5581 gttgccgttt gtggagacgg gcgagaggtc ggtgctgacg ccggcctcgg tgttgagcag
     5641 ttgggtgaag ctgcgcgtcg aggtcggcac ctgaacgagc tgctcggcgg taatctggcg
     5701 ggcgatggtc gccgtctcgg gcgtggccag cacctcgccg cccgtgaccg tgacgctctc
     5761 gccggtgatg tctccgattt cgagcttgtc ctcgaaggtg cgggggacgg cggcctcgac
     5821 cgccgcgtcg ccgcgcttga gcggcttgaa gccggcgagc gtgtaggtga tctcgtagac
     5881 gccgacgccg aggcccggca tcgtccagcg cccctcgtcg ttggtcgtgg cggcgcgcgt
     5941 ctgtcccgtg ccggtgtttc gcgcctcgac ctgaacgccc ggcagggccg cgccgttggg
     6001 gtctgtgacg gcgccggtca gcgtccccat cgggcctgac tgcgcgcgag ccggcgccgt
     6061 ggcggcgacg agggcgagcg cgaaaaggag cagcaggcag acgcccgccg ccgccacggc
     6121 gcggcccgac ctggatacgt tggggttgat ttctcgcatg gggattctcc tgtcagactt
     6181 aaaaaacatt cgggtgaaaa acgattgcct cgcggcgcgc gcgcacgtgc ggcggcccga
     6241 aaggatgatt gccgagttgc tctatttggc ggcgcgttga aaagcagccg cacgcgtgga
     6301 agtgttgatt caagcgtgag aagttaacgc gccggagtta agggcgggtg aatccggtct
     6361 taattaagtg ttaagagaat gagggagtga ataggctatg ttatcaaaga gttacatgac
     6421 cggcccgctc tcgcctggtg cgaacgaggg gcgggaggcc ccaaaaatct gcctttccag
     6481 cgtctcggcg accccctcga acatctgcgg gagcgtcctg acgtagaccg cccggttgaa
     6541 gtcctcgcgg gcgtggccca tgaggacgca ccgagccttg tcggccacgc cgccctcgct
     6601 gagcagcgtg ttgtaggtgt aacgcaggga gtaaggcgtg aagtgagggg gaagccccga
     6661 cctcttcagg agccgcgcga acatgtcgcg cagccacccc tcgctgatgg gcctgcccgt
     6721 ccgggaaggg aagacgaggc cgtagtccgt ccacccggcg ccgaggaggc gctctcgggc
     6781 cgcgttcgcg cattggagcc tcatcaactc gcggtagagc cacgcgggga agggcacctc
     6841 gcggacaccc ttgcgcgtct tcggcctcgt gaagatgtgg cccggcgcgc ggcggagctt
     6901 gacggcctgc tgcgtcaccc tcaccagcgc cgccgcgtcg tccacgagtt cgacgttgga
     6961 gagcggaagc cccaggcatt ccttcggcct catgcccatg aagagcgcga agacgaagat
     7021 gagatcgccc aagtcgagca gggccgtctc gacgagcgtt cgcgcctccg cgggcgtgag
     7081 gcttcgcgtc tcccgcggct ccgcgccctt cgggagttcg agaccctcca gcggactctc
     7141 tctcaaatac tttttccaga cggcgtactt gatgaagagt ttgcagaccc gccggacgtg
     7201 cttgatggtc gagtccgaca gcccccggtc ttgcaggccg ttgaacatcc tttcgagcgc
     7261 gcccggctga aagtctttaa tctggaggtg tccgacgtaa gggagcacgc aggagttgag
     7321 cgtgtcccgg tagttgtcta tcgtggtgac ggcgaggccc ttgcgcctgt gttgtttcat
     7381 ccactcttcg gtgagggcct tgacggtgag gggcgacggg acgaaaagca gcccggcgcc
     7441 gatgagcgtc acgaggtctc tgcgccgggc gccggccttc tccggcgtcg tgtcgtagag
     7501 catttccgag ttcgtgatgg ggcgtcgggt gcatgccgtt gtcaggtggc gtgttgggca
     7561 gaatgtgtca ccgccgcagc cgggagcggc ctaacttaat agaacctcac gcatgactat
     7621 tgccaggtct cgcaacgcgc gctccccatc gcccgtgaag gcagagccgt gcatggcggc
     7681 gatggtcttc ggcttgagat cggctaagct gtgtagggtt tgatccgtgt gctgcgtgta
     7741 cggcatataa ttcgcgaaag ggcctgcctg atagtcaact agtgtcttac gcacagcttc
     7801 aatcacgtcc gattcagtca acggcgcgac gttcccgttc tgatgaaaca ggtcggaaca
     7861 aagaagggtt ttgttctcct cttcaaagag caacccggcc tcccagcagt gcggcacgtg
     7921 cggcgtgtgg agcaggcgga agcggtactt gcccgtgtgt agaatctccc cgtcagacat
     7981 gcctttggcc gggcggacgg caaaatcgtt gacgctgacg agcgccccga ccatgctgca
     8041 cacgggctgg gcggccgggg cctgctggag ccattcgttg agcgcgccgc actcgtccgc
     8101 ctcgaaatgg ctgaagccca gccaccgtat cctcgaagga tcaattaggc tcgccaccgc
     8161 atcgcggacg aagggaaaca tcgcccgatg tccggtatga aacagtagtg gctcatcgtc
     8221 cctgacgaga aattgattga actgcatgtc aatctccggg acataagtag agatgcggta
     8281 tacgtcaggg gcaatctccg tcacactcat ggcctacctc ccaaattcca ttgtccacga
     8341 ccatcatatc tgctaacgcg aaatttctgc tgaacctgga tcactcgccc aacaggaagc
     8401 catctaacgc ccggtatcac ccgccgcgca cgcaacgccg cttgagaaca agccccttgc
     8461 gatgccgtcc gcgcctgtgc cttggcaacg gtgtacgttg cgcgtgtcag tgtttcccgt
     8521 tgctgccgga ctccgtgacc ccgccttgtg caatcaaggg ttgttgccgg ccgggtacac
     8581 cttgatccaa tgggtacgca cctgcggctg caccattcgc ccctcgttcg tcgaaacgac
     8641 ggacttcgac gtgaccttcg gcatgtggca gttgatgcag ttctcggcga tcttgtggcc
     8701 cctctcccgg taaatcccac aactctcgac cttgtggcac gtcagacaac tgcgcgagaa
     8761 ggccgccgcg tcccgctgcg gtagatgaac atcgtggcac gttgagcaag acatggtgga
     8821 ggcttgaaag cagcggctcc tctccagaag agccacctgg ttgccgtgaa catcgatcac
     8881 ctcatccggc cccgaggctc cgcgctggat gtaatcaccg agcggcttgc ctgccacgta
     8941 ggaaaacgcc ggggccttgc ccaccccgat gccgccgtga cacagggcac acgcttcgac
     9001 ctgccgggcg cgagaaagtt ttgccggatt gataatcgcc tgtcccgaag atttggcggc
     9061 ctgagaggtt tggaccgcgg agtgctcttg gcccggaccg tggcacctcc cgcaagagat
     9121 gcctagtaca taattggcct tgtcgtatcg attgccggcg cccgacccgg agagcgactc
     9181 gaagtatgag gcatggcact ccagacagcg tggggcgacg ggacggccga aatttaattc
     9241 cccatcctca taccccggac tattgaccca tttccccggg tcagtccagt acgagacggg
     9301 cagctgaaag agtccgtcgc cctcccccca gtacaggtac gactgccctt tccgcccgga
     9361 gccgatcacg aaatcgaacc gcacggagag ggatgcggtg tcggggggcg tgcccagcac
     9421 ggcggtctgg taaaagccgt ccttcctcgc ctccatcctg taatacaggt ctggattcga
     9481 tgtcctgaga atgttttcgc cctcacgaaa tttaccggcg attgtatctt tcgtcggcgc
     9541 cgccgaggtc cgatagtggg ccgtttcccg aaaggtctta tggtctcggt ggcaagacag
     9601 gcactgattg tctccggcgt aatccccggg cctgacccgc tcatagcctt gcggggcaga
     9661 ggccgcgagg gccgccaagc acgccgtgaa gattagacat agcgagagcg cggcgaagtc
     9721 actggagggc ttcctcccgc tgatccacct tcgagaagca cggtcactga ggtcctcaac
     9781 aggttccaat gtcaccccag ctcaggcgtc ttttccggca gccgaactga aaataatgac
     9841 ggcggaggct actccttgcc cccaccgtca tccactcatg tccttacgtg aggccacgtc
     9901 ggacacttcg ccgattggtg cggagggcgg ccgttgaggc gcccgctctg acggcgcgca
     9961 ctatggcacc gccgggccga ccctgaattg acccttacgc acacgcgcgc cccacgaacc
    10021 cggctcccac ctccgcctgc acaacacagc ctctgaatcc agacagctgt gccgacgtac
    10081 cgtgacgtcc cgtcttatct gcccagagca gaaagaggag gcgcatcaac gggcagtggc
    10141 catgtcggag ggtttgatta acgcctcgcc tcgacacgat cacctggcgt aatccgtggg
    10201 tcgggcactt tgcccgatag gatgtcgcgc atgttgtagg tcgtcgaggt caaaagtccg
    10261 ccgcctttcg gcgactgtgc gcccttatcc tcgggggggg cgataacgcc cgtttgagga
    10321 acattacggt tcgaccacag ggtcacggtc cgcaaccggt ggagctcgcc gcctgcggcg
    10381 ataatcgcct gcgtgagagt gatctccggg tgaaactcct tctgccctgg gctgaccacg
    10441 ccgccgctga cgtagtagaa ctgctgcgac attttggtca ccatgagcac atcgccccga
    10501 cgaacaggca cggccatcgc cgcctggtcc tcaagcgcca cctcggttcg tagcccggtg
    10561 gcgtgggacg tgaccaacac gcgcccagcc tcggggagag gctgcgcgtc ggcgatcagg
    10621 acgtagagag gtacttcctc ccgcttcagg atcttcgtgc cgggttccgc gacgagcccg
    10681 ctgacgatga cggcgtgact aacgtactca cgcacattga ccaacaccct ggggtcatcc
    10741 tcgatggccc gtcggcgcaa ctcctgtgcg atgcgctccg cggcctcctc caccgtttgg
    10801 ccttcgacgc ttagcggctc gctgaagagc ggatactcca aaagtccacc gggcaacacc
    10861 ctgtagaggg tagatttgtc cgtccgttcg ttgagcaggc gtatgtctag tacgtcgccc
    10921 acccccaggc ggtagagatc ggacggcggc ggggcgacct tcttctcagc gtgggcggtg
    10981 tcggtcctta gcggcacgac cttagcggtc ggggcgttcg tcaccgccgg ctcaacctca
    11041 ggagtcaccc ccttgtgggg cggtgcggag ttattcttcg cctccgccgc gggagggtcg
    11101 gtagatgcat acctgttgtc ttccccaacg agccctctca ccacgtcgct cctactgagc
    11161 cagcgcagac cctttgagtc tgtcccaacc accccggtgt tgactagcgc cacgtgctcc
    11221 ccggtggcgc cccctccgtt aaatgatgtc tggcgaagat tactctggat gaccggatca
    11281 ttcggggcga ggcgcaccgc gcgctcgagt gctttagccg catcggcgta ccggcccaca
    11341 aggtagtaag cgatccccag attgttgtgg gcgtaggcat cgtcggaatc attgtccgtt
    11401 gccagccgca aggcctccag cgccttctca tatttgcccg actcgccgta ggcggctccg
    11461 aggtcgttgt agagttcggc ggtggcgggc ctcaaggatt taatatccgc gtaatatgtg
    11521 gccgccggct cataacgccc catccggtga aacgcgttgg cgatgttgta gcgcacatcg
    11581 gcggacttgg gtttaataag gatggcgcgt ttgaaagctt cgacggcctg ctcatatagc
    11641 ccctgcctga agcgtgatgc gccgaggtag acgatgctgt cgaagtcttt cggattgagg
    11701 gcgacggcct gcatgaaggc ctccgcggcc tccttgaagc ggcccgcgtc atagtaggcg
    11761 tggcccagac cgaagtgcgc gtcggcgtac gtcggcttgt agagaatcgc ctccctgaaa
    11821 gcttccattg cctccttgta gcgcccagca agtccgtacc taacccccaa atcatagctg
    11881 gccttcgagg cctcctcgtg cgcgtggccg cgcaccggag ccacgccggc gggtgcctta
    11941 tcacctgaag tgagaatatt gacctcgctt atcacccggg actttggtga tgggcccgac
    12001 gtggcttgtg cccgcacagg aaactgtgag acgcagatga tgatcagaac gaggcaagag
    12061 cgtgagacaa cttctatcat gatccactcg tcctttcgag gaggaacgca caccaccatt
    12121 ggtaagcacg caacgaccgg ggcagcaggt cacatcagga tgggagagac ttcacgcttt
    12181 tccgagctca tccatcgacg tggccgacgc caacgcaaca tcggctcacg tactgggcaa
    12241 tgtgcgtgag ccgatgggtt atttgtttga gtggccggaa ggatctaacc caccgaatgt
    12301 gttgctgttg aaacccctca gccgtgtctt caggcacccg cctgccgacg actgcgcacc
    12361 ttggcggcga tgcccgccag ccccgtcccc a
//
LOCUS       DASVES010000078        11096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1231803, whole genome shotgun sequence.
ACCESSION   DASVES010000078 DASVES010000000
VERSION     DASVES010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231803"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1434)
                     /gene="coaBC"
                     /locus_tag="VF240_02830"
     CDS             complement(124..1434)
                     /gene="coaBC"
                     /locus_tag="VF240_02830"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7174209.1"
                     /translation="MSNEDSQVNAEADERGPAATEAGRRVALGVTGGIAAYKAAEILR
                     GLQRAGCSVRVCMTRRACEFITPLTFRALSGAHVVVDDYAPDNPDPIAHITFSQTVEL
                     FVVAPATADIIAKFANGIADDFLSTTYLASTAPVLVAPAMNTVMWNHPATRRNLERLR
                     ADGVRVVEPDEGEMACGAFGPGRLSEPGVIVEAALEMLRAGARGAAGNAASVGAGDLS
                     GERVLITAGATREEIDPVRFLTNRSSGRMGFALAAAAAARGAEVTVVAGATTADAPAG
                     VRVVRALSAEEMRGAVLREIESATVFIAAAAVSDYRPAKRAASKIKKTDAGLTLELER
                     TPDILAEVALARHNGLLVIGFAAETDNLVENARQKLLRKKLDAIVANDITREGAGFDG
                     DTNVITLLARDREGHVELPLMSKSEAAGRILDEVLRLRRGAQTK"
     gene            1639..2253
                     /locus_tag="VF240_02835"
     CDS             1639..2253
                     /locus_tag="VF240_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174210.1"
                     /translation="MKTLKRHLRWPLCLLLCLICAVSASAQKKSSARAAAEEDEFGPI
                     VRAYLGYLRNQQEVVDDRVSRREVDRAYYVRNSNRIRALRHMAVKIARETENDYLPEL
                     EAVTQDEFGLLFDLPPELHQLQAGEVLGYQLRFIGATVSGRDKFYVFARLDPYEQAEL
                     RKNAAPPTPKMTPAPAAATAAEQQPAATVPANTSRPRRATPPRR"
     gene            2279..2629
                     /locus_tag="VF240_02840"
     CDS             2279..2629
                     /locus_tag="VF240_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174211.1"
                     /translation="MRQPTFFDKGAGDDGKAPDTESIVLHALGEFQARGKLLADRELP
                     LDRLRGALRRACDARGVSLLDDEQAAAAFSQLGAHVRRVASFVAKHPFRVTVPPDLAA
                     RAREFFDQRGDDRS"
     gene            2840..4453
                     /locus_tag="VF240_02845"
     CDS             2840..4453
                     /locus_tag="VF240_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7174212.1"
                     /translation="MAGSIVEGRKVGENRTLTPEFCIIGSGAGGGVCALKLAEAGFDV
                     LVLEEGGNVPEGQGHGGTSHRRPSFTEREFDMYKTLYQEGAARLTSDGGVKVMQGRCL
                     GGGTTVNWSACLPPRTETIEHWRNHFGLHFTPDNLRPYLLEVVNFLHIHRNDRYNSSA
                     QALIRGCTALGYHWGNLPNNTHNCRECGSCGVGCPYDRKQSGIVTWLPAAVARGATIY
                     TDTKVDRLVRDGERPPEVHAAFVDEKTQATGRTLIVKPRLGVVLAAGAVGTPSILLRS
                     NINPNKLVGRRTHIHPVTICIGRYPERTHPAYGVPDNMMSTQFASGPVGYLIETGSFF
                     PVLNAAASLDYGPELHRVMREYYPFGAIMYAHHCSGFDPKQDYGTVKLDKYKDPELEY
                     RLAPDNARAMKHSLVEMTKIHLAAGAKSVYHVTNPSIEIKSVAELGKLDDISFEPQRA
                     TVFTVHVMGGCPMGAAEKQSVVATDFSLRETPNVWVADGSIFPTGLGANPQVTIYALA
                     LRAARFICEKHGKPFTLNHQQPGELPWDF"
     gene            4441..4974
                     /locus_tag="VF240_02850"
     CDS             4441..4974
                     /locus_tag="VF240_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174213.1"
                     /translation="MGFLVPFFNFFINPKKVIQIDESKLKHLNEKSAHILFALAEAVI
                     GEEFRVKVPKFVEMIDDYLGYQRRSQREALNQGLLIVESTIVTLVFGRRLRGFSHLSL
                     PDRRRVLENMRQSRVQQFRNLYAAAVNISASAYFASEATWAEIMYAGVSVDHPEILTG
                     PPPPVRWRPNDTRPVEP"
     gene            5154..6371
                     /locus_tag="VF240_02855"
     CDS             5154..6371
                     /locus_tag="VF240_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEX7174214.1"
                     /translation="MSTKKSRRKFLQGAAAGLGAATLAPRVGVEVLATGGQGSQTAAQ
                     LRSQPPLVVSSANGVAAVTKAVQELKKGADTLDAVIAGVNINELDPKDDSVGYGGLPN
                     EHGDVELDASVMHGPTRRAGAVASLRGVRTPSKVAKAVLENTDHILIVGQGARDFATQ
                     MGFENVNLLTEESRIAWQRWKETTSDDDDWGPQKLRPAPASPAGRSKASLDQLSPSFR
                     AADEYARGDVKLRDALLTRADFVARHPPTGTINCIALDRNLDISGVTTTSGLAWKIPG
                     RVGDSPLIGCGLYVDNDIGAAGSTGRGEEVIYVNGARTIVEYMRQGKSPEQACLDVLK
                     LIVARYGNDPVMLKQFDVNFYALNKKGDFAGAAIWSHSVFAGGERRRRQFAAAAGGGE
                     GRLYESAYLFERR"
     gene            6684..7397
                     /locus_tag="VF240_02860"
     CDS             6684..7397
                     /locus_tag="VF240_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174215.1"
                     /translation="MISRNLSRKTITLALALTVWSTYSMATLAAQGQQAQSGDLSVVG
                     EVTVNGASAISGATVFSDSTVTTAKGSSAVVSLGKLGRVEVLPESSLKLSFNETGITA
                     MLDSGRVRLSTSSGTGASVTTKDATAVADNNQPNVFLVDVECGNTQVSTQSGRVELRA
                     GNAVKQIAAGSQDTAGQATPGTRCTRLAMPDMEGLGGGALAALLLATGGAILAAFLAS
                     RENDDFQFGDDVIVISPAR"
     gene            7561..8859
                     /locus_tag="VF240_02865"
     CDS             7561..8859
                     /locus_tag="VF240_02865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX7174216.1"
                     /translation="MHIAVIGTGYVGLVTGACFAEFGVDVTCVDVDADKIARLSAGEI
                     PIYEPGLDKIVEKNMKAGRLHFTTDLGAAVEESLVIFLAVGTPPKEDGSADLSFIEEA
                     ARQVSEHMNGYKVIVTKSTVPVGSGEHLRRLIQEHQKQRHNFGVVSNPEFLREGAAIN
                     DFMRPDRVVIGTRDEEAIAIIRDLYRPLFLIETPFVITSLEAAELTKYAANAFLATKI
                     SFINEIASMCDRIGCDVHDVARAIGMDKRIGKYFLHPGPGYGGSCFPKDTSALLSVAR
                     AFGADAHIVESVIKVNQLQREAMVPKIEQLTGGLEGKIVAVLGLSFKPETDDMRDAPA
                     VDIIRGLLARGARVRAYDPVAAPQASKILPEIEYADDEYAAVTGADALVFITEWNQFR
                     ALDMERIRGLMRSPKIADLRNIYEPSAMREMGFEYVGVGR"
     gene            8983..9942
                     /locus_tag="VF240_02870"
     CDS             8983..9942
                     /locus_tag="VF240_02870"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7174217.1"
                     /translation="MALTGIYLVTGGAGFIGSNIAGTLAERGARVRIVDDLSTGHQEN
                     IEEIGGHVDFIRASVTDYDAMRRALDGVEVVFHEAAIPSVPRSVDNPLETHRACVDGT
                     FSLLTAAREAGVRRVVYAASSSAYGEQPTLPKVEDMAPQPLSPYAAAKLVGEYYCQVW
                     TRTYGLETVCLRYFNVFGPRQDPSSQYSGVISRFIDALSTATQPVIYGDGEQSRDFTY
                     VSNAVDANLLAAESTRAVGQVINVANGQRVTLNELLDRLKKITGRTEAEAEFRETRAG
                     DVRHSLADITRARELLGFEPQVGLEEGLRRTIDWWRQSRFAQR"
     gene            complement(10076..>11096)
                     /gene="mtnA"
                     /locus_tag="VF240_02875"
     CDS             complement(10076..>11096)
                     /gene="mtnA"
                     /locus_tag="VF240_02875"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX7174218.1"
                     /translation="EGVRMIDQRLLPTEEKYLTFRSCDEVAEAIKLMVIRGAPAIGVS
                     AAMGIALGTKQSVGTSVADLEFDFDYNCDLLGRTRPTAVNLFWAIERMRDCFQRAKAE
                     GADAEAIKRRLVEESQAIFKEDVESNRALGRFGGELIPDGATVLTHCNAGALATAGDY
                     GTALGVIRGARDAGKRVAVIADETRPFLQGARLTAWELAKDEIPVTVITDNMAGHVMK
                     QGKVDAVVVGADRIAANGDTANKIGTYMVAVLAREHAIPFYVAAPVSTVDLKLSSGEQ
                     IPIEERDEREVTHIRDQRLTPEGVLIQNPAFDVTPNEFIAAIITDRGVARQPFTESLR
                     RLVEG"
BASE COUNT         1992 a         3689 c         3506 g         1909 t
ORIGIN      
        1 acttaactgc gattcatttt tcattagcgg cggcgcaatt atcgcacgat tccgacgcgc
       61 ttgtgtagac gaccgtaaca gtcgccgcca gcgccgcgtt ttgtcactcc gcgccgcgcg
      121 aggctacttc gtttgagcgc cgcgtcgcag acgcaacacc tcgtcgagga tgcgcccggc
      181 cgcttcgctc ttggacatga gggggagttc gacgtgtccc tcacggtcgc gtgcgagcag
      241 cgtgatgacg ttcgtgtcgc cgtcgaagcc cgcgccctcg cgggtgatgt cgttggcgac
      301 gatggcgtcg agtttcttcc gcagaagttt ttgccgcgcg ttctcgacga ggttgtcggt
      361 ctcggcggcg aagccgatga cgaggaggcc gttgtgtcgc gcgagcgcaa cctcggcgag
      421 aatgtcgggt gtccgttcaa gctcaagggt caggcccgcg tccgtcttct tgatcttgct
      481 cgccgcgcgc ttggccgggc ggtagtcgga gacggccgcg gcagcgatga agacagtggc
      541 gctctctatc tcacggagca cggccccgcg catctcctcg gccgagaggg cgcgcacgac
      601 gcggactcct gcgggtgcgt ctgctgtggt cgcgccggcc acgaccgtga cctcggcccc
      661 gcgcgcggcc gccgccgcgg cgagggcgaa gcccatgcgg ccggaggagc ggttggtcag
      721 gaagcgcacg gggtcgatct cctcacgcgt cgcgccggcg gtgatgagca cgcgctcgcc
      781 cgacaggtcg cccgccccga cgctcgccgc gtttccagcc gcgccgcgcg cgcccgcgcg
      841 cagcatttcg agtgcggcct cgacgattac tcccggctcg ctcagacgcc ccggcccgaa
      901 ggcgccgcac gccatctcgc cctcgtcggg ttcgacgacg cgcacgccat cggcgcgcag
      961 ccgctcaagg ttgcgtcgcg tcgccgggtg gttccacatc accgtgttca tcgccggggc
     1021 gacgaggacg ggggccgtcg aggcgaggta ggtggtcgag aggaaatcgt cggcgatgcc
     1081 gttggcgaac ttggcgatga tgtcggcggt ggcgggggcg acgacgaaga gttcgacggt
     1141 ctgggagaag gtgatgtgcg cgatggggtc ggggttgtcg ggcgcgtagt cgtccacgac
     1201 gacgtgcgcg ccggagagcg cgcggaaagt gagcggggtg atgaactcac aggcccgcct
     1261 cgtcatgcag acgcgcaccg agcagcccgc gcgctgcaac ccgcgcagga tttcggcggc
     1321 cttgtaggcg gcgatgccgc ccgtcacgcc gagcgccacg cgccggcccg cttcggtcgc
     1381 cgctgggccg cgttcgtccg cctctgcgtt tacctgtgaa tcttcgttgg acatgtgcgc
     1441 gcctgagttt gaatttatca cccgcgcgcg cggcgccgca aataacgtgc gatgtgaagc
     1501 gaggcgcgcg gccattcgct ctcgcggccg gggccgtttg tgctaaactt cgcgcgtaga
     1561 cacccccacg catccgtcag gcgcgacaaa cccccggccg gtcgagcctc tgacacgaac
     1621 cgagtgcatt tttaccgaat gaagacgctg aagagacatc tccgctggcc tctgtgcctc
     1681 ctgctctgtc tgatatgcgc ggtctccgcg tccgcgcaga agaagagttc ggcgcgagcg
     1741 gcggccgagg aggacgagtt cgggccgatt gtcagggctt atctgggcta tctgcggaac
     1801 cagcaggagg tggttgacga ccgcgtcagc cggcgcgaag tggatcgcgc ctactacgtg
     1861 cgcaactcga accgcatccg cgcgctccga cacatggccg tcaagatcgc gcgcgagacg
     1921 gagaacgact acctgcccga gcttgaggcg gtcacacagg atgagttcgg cctcctgttc
     1981 gacctgccgc cggagttgca ccaattgcag gcgggcgagg tgttgggata tcaactcaga
     2041 ttcatcggcg caactgtatc gggccgcgac aagttctatg tcttcgcgcg cctcgacccc
     2101 tacgagcagg cggagctgag aaagaacgcc gcaccgccga cgccgaaaat gacgcccgca
     2161 cccgcggcgg cgacggcggc cgagcaacag cccgccgcca ccgtccccgc gaacacgagc
     2221 cgcccgcgcc gcgcgacacc gccccggcgc tagacggctg caacccgacc gacttctcat
     2281 gcgccagccg accttcttcg acaaaggcgc gggcgacgac ggcaaagcgc ccgacaccga
     2341 aagcatcgtg ctccacgcgc tcggcgagtt tcaggcgcgc ggcaagctgc tggccgacag
     2401 ggaactgccg ctcgaccgcc tgcgcggagc actgcggcgc gcctgcgacg cccgcggagt
     2461 ctccctgctc gacgacgaac aggccgccgc cgccttcagt caactcgggg cacatgtgcg
     2521 gcgcgtcgcc tccttcgtcg ccaagcaccc cttccgcgtc accgtcccgc cggacttggc
     2581 cgcgcgggcg cgcgagtttt ttgaccagcg aggagatgac cgaagttaga cactgacttt
     2641 cttgtctgcc tcaacgtttg gtgctagagt ccgccccgtc cgtttttctc gaccctcgcc
     2701 gctacattcg acacgtgctc tcaccgtcgc ccattgccct taattctcaa ggggtttctg
     2761 tcccgcgcca ttaagaaaca cacttgactt ttgaggtgac gaattgcttt tgctcgttct
     2821 ttccaggagg agataagaca tggcaggcag catcgtcgaa ggccggaagg tcggggagaa
     2881 tcggacgctc acgcccgagt tctgcatcat cggctcgggg gcgggcggcg gcgtgtgcgc
     2941 gctcaagctg gccgaggccg gcttcgacgt gctcgtgctc gaagagggcg gtaacgtccc
     3001 cgaagggcag gggcacggcg gcacgagtca ccgccgcccg tccttcaccg aacgcgagtt
     3061 cgacatgtac aagacgctct atcaggaggg cgcggcccgc ctgacgagcg acggcggcgt
     3121 caaggtgatg cagggacgct gcctcggcgg cggcaccacc gtcaactggt cggcctgcct
     3181 gcccccgcgc accgagacga ttgaacactg gcgcaaccac ttcggcctcc acttcacgcc
     3241 cgacaacctg cggccctacc tgctcgaagt cgtcaacttc ctccacatcc accgcaacga
     3301 ccgttacaac agctcggcgc aggcgctcat ccgcggctgc accgcgctcg gctatcactg
     3361 gggcaacctg ccgaacaaca cgcacaactg ccgcgagtgc gggagctgcg gcgtcggctg
     3421 cccctacgac cgcaagcaga gcggcatcgt cacgtggctg cccgcggccg tcgcccgcgg
     3481 cgcgaccatt tacaccgaca cgaaagttga ccggctcgtc cgagacggcg agcgaccccc
     3541 cgaagtccac gccgccttcg ttgatgagaa gacgcaagct accgggcgca cgctcatcgt
     3601 caagccccgc ctcggcgtgg tgctcgcggc cggggccgtc ggcacgcctt ctatcctgct
     3661 gcgctcaaac atcaacccga acaaactcgt cggccgtcgc acgcacatcc accccgtgac
     3721 catctgcatc ggtcgctacc cggagcggac acacccggcc tacggcgtcc ccgacaacat
     3781 gatgagcacg cagttcgcga gcggccccgt cggctatctc atcgagaccg gctccttttt
     3841 tcccgtcctc aacgccgccg cctcgctcga ctacggcccc gaactccacc gcgtcatgcg
     3901 cgagtattac cccttcggag ccatcatgta cgcgcaccac tgctccggct tcgaccccaa
     3961 acaggactac ggcaccgtca agctcgacaa gtacaaagac ccggagcttg agtacaggct
     4021 cgcgcccgac aacgcccgcg ccatgaagca cagcctcgtc gagatgacga agattcacct
     4081 cgccgccggc gcgaagtccg tctaccacgt caccaacccc tctatcgaaa ttaagtccgt
     4141 ggcggaactc ggcaagctcg acgacatctc gttcgagccg cagcgcgcca ccgtcttcac
     4201 ggttcacgtg atgggcggct gcccgatggg cgcggccgaa aagcaatccg tcgtcgccac
     4261 cgacttcagc ctgcgcgaga cccccaacgt ctgggtggcg gacggctcca tcttcccgac
     4321 gggcctcggc gcaaatccgc aggtcactat ctatgcgctg gcgctcaggg ccgcccgctt
     4381 catttgtgag aagcacggca agccgttcac cctgaaccat caacagccgg gagagttgcc
     4441 atgggatttc tagtgccgtt cttcaacttc ttcatcaacc cgaagaaagt gattcagata
     4501 gacgagtcga agctgaagca cctgaacgag aagagcgccc acatcctctt cgcactggcc
     4561 gaggccgtca tcggcgagga attccgcgtc aaggtgccga agttcgtcga gatgatcgac
     4621 gactacctcg gctatcagcg ccgctctcag cgcgaggccc tcaatcaggg gttgctcatc
     4681 gtcgagagca ccatcgtcac cctcgtcttc ggccggcgcc tgcgcggctt ctcgcacctc
     4741 tccctgccag accgccggcg cgtcctcgaa aacatgagac agagccgcgt ccagcaattc
     4801 cgcaacctct acgctgccgc cgtcaatatc tcggcctccg cctacttcgc cagcgaggcg
     4861 acctgggccg aaatcatgta cgccggcgtg agcgtcgatc accccgagat tctgacgggg
     4921 ccgccacccc ccgtgcgctg gcgccccaac gacacgcggc cggtcgagcc atagtcagtc
     4981 gagtgtcgaa gagagatgcg tggtgacgca ttcatcgccc acatcgaccg cctgagaaga
     5041 aatctgttgc gcctgcctgg ggcctttgtt accatgcgcc ccacattcct tcagtcccgc
     5101 tgatgttaaa aactcgcgcc ggcgtctgcc gagacggagc catgagcatg tcaatgagca
     5161 cgaagaagag ccgtcggaaa ttcttgcagg gcgcggccgc cgggctgggc gcggcgacgc
     5221 tcgcgccccg cgtcggcgtc gaagtcctcg cgacgggagg gcaggggagt cagacggccg
     5281 cgcagctacg ctctcagccg ccgctcgtcg tgtcgagcgc gaacggcgtc gcggccgtga
     5341 cgaaggccgt gcaggaattg aagaaagggg cggacacact cgacgccgtc attgccggcg
     5401 tcaacatcaa cgaactcgac ccgaaggacg actcggtcgg ctatggcggt ctgccgaacg
     5461 agcacggcga cgtggagctc gacgccagcg tgatgcacgg gccgacgcgc agggctgggg
     5521 ccgtcgcctc cctgcgcggc gtccgcacgc cctcgaaggt cgccaaggcc gtccttgaaa
     5581 acaccgacca catcctcatc gtcgggcagg gcgcgcgcga tttcgccacg cagatggggt
     5641 tcgagaacgt caatctcctg acggaagagt cgcgcatcgc ctggcagcgc tggaaggaga
     5701 cgacctcgga cgacgacgac tgggggccgc agaagctacg ccccgcgccc gcctcgcccg
     5761 ccgggcggag taaagcgtcg ctcgaccaac tctcgccgag tttccgtgcg gccgacgagt
     5821 acgcgcgcgg cgacgtaaaa ctacgcgatg cgctcctcac gcgcgccgac ttcgtggcgc
     5881 gtcacccccc gaccggcacc atcaactgca tcgccctcga caggaatctc gatatcagcg
     5941 gggtgacgac gacaagcggt ctggcgtgga aaattcccgg ccgtgtgggc gactcgcccc
     6001 tcatcggctg cggcctttac gtggacaacg acatcggcgc ggccggctcg acgggtcgcg
     6061 gtgaggaagt catctacgtc aacggcgcgc gcaccattgt cgagtatatg aggcagggga
     6121 agtcgcctga gcaggcctgc ctcgacgttc taaagctgat cgtcgcacgc tacggcaacg
     6181 acccggtgat gctcaaacaa ttcgacgtga acttctacgc cctcaacaag aaaggcgact
     6241 tcgccggggc cgccatctgg agccattcgg tcttcgccgg gggcgagcgc cgccgccggc
     6301 agttcgccgc cgccgctggc gggggcgaag gccggctcta cgagagcgcc tacctgttcg
     6361 agcgtcgcta aggcaagagt tgtgtcaaaa atgttttgcc aaaagggtcg caagcgttgg
     6421 gctatgagcg gtttgcggaa gaaaaaagct taggttttcc gtaaatgtat gttgacaccg
     6481 ggccgggttt cgtgtaaaaa agcggggtct attgaagccc gctgcggctc tcatcctgca
     6541 tttttgtgag atgttcttcg cggtgcttga ttctctgaga cgggtaggcg acataaacca
     6601 aaagaaattc gtctcctgcg agtccacttc gagacttgcg gcgctctcta aaaattttga
     6661 aggtttcact acgggaggaa gaaatgatta gcagaaattt gagccgtaag accatcacgt
     6721 tggccctggc gttgaccgtc tggagcacgt actcgatggc cacgctggcc gcgcaggggc
     6781 aacaggctca gtcgggcgac ctgtcggtcg tcggtgaagt taccgtcaac ggtgcgagcg
     6841 ccatctcggg cgccaccgtc ttctccgaca gcacagtcac caccgccaag ggttcgagcg
     6901 ccgtcgtcag cctcggcaaa ctcggccgcg tggaagtgct tccggaatcc agcctcaagc
     6961 tcagctttaa cgagaccggc atcaccgcca tgctcgactc cggccgcgtt cggctctcga
     7021 cctcttcggg gacgggcgcc agcgtgacga ccaaggacgc cacggccgtg gccgacaaca
     7081 atcagcccaa cgtcttcctc gttgatgtcg agtgcggcaa cacgcaggtc tcgacgcagt
     7141 cgggccgcgt cgagcttcgc gccggcaacg ccgtcaaaca gatcgcggct ggcagccagg
     7201 acacggccgg tcaggccacc cccggcaccc gctgcactcg cctcgcgatg cctgacatgg
     7261 aaggccttgg cggcggcgcg ctggcggctc tcctgctggc caccggcgga gccatcctcg
     7321 ctgccttcct ggcgtcacgc gagaatgacg atttccaatt cggcgacgac gtgatcgtca
     7381 tcagcccggc ccgctaacgg ccggttaaca acggaaactc gccgcgcttt aagttccgca
     7441 cggcgataac cagagccttg catttcttgc gggtcacgcg gatcatggta cgcgtgaccc
     7501 gcatactttt gtgtggggcc gcccaggaat aatccctgcg aaaggcttaa ccaaccaaga
     7561 atgcacatcg cggtaatcgg cacgggctac gtcggcctcg tcactggcgc gtgcttcgcg
     7621 gagttcggcg ttgacgtgac ctgcgtggac gtggacgccg acaaaatcgc gcgtctctcg
     7681 gcgggcgaga tccccattta cgagccgggc ctcgacaaaa tcgtcgagaa gaacatgaag
     7741 gccgggcgcc ttcatttcac gaccgacctc ggggcggccg tcgaagagtc gctcgtcatc
     7801 ttcctcgccg tcggaacgcc gccgaaggag gacggctcgg ccgacctctc gttcatcgag
     7861 gaggcggccc gacaggtctc tgagcacatg aacggctaca aggtgatcgt cacgaagtct
     7921 actgtccccg taggctccgg cgagcacctg cgccgtctca tccaggagca tcaaaagcag
     7981 cggcacaact tcggcgtcgt ctcgaacccg gagtttctgc gcgagggcgc ggcgatcaac
     8041 gacttcatgc gtcccgaccg tgtcgtcatc ggcacccgtg acgaggaggc catcgccatc
     8101 attcgagacc tctaccgccc gctcttcctc atcgagaccc cgttcgtcat cacctcgctc
     8161 gaagccgcgg agttgacgaa gtacgccgcg aacgccttcc tggcgaccaa gatttccttc
     8221 atcaacgaaa tcgccagcat gtgcgaccgc atcggctgcg acgtacatga cgtggcgcgc
     8281 gccattggca tggacaagcg catcggcaag tacttcctcc accccggccc cggctacggc
     8341 ggctcgtgct tcccgaaaga cacctcggcg ctgctgtccg tggcgcgggc cttcggcgcc
     8401 gacgcgcaca tcgtcgaatc cgtcatcaaa gtcaatcaac tccagcgcga ggcgatggtg
     8461 cccaagatcg agcagttgac gggcggactc gagggcaaga tcgttgccgt gctcggcctc
     8521 tcgttcaagc ccgagacgga cgacatgcgc gacgctcccg cggtggacat cattcgcggg
     8581 ctgctggcgc gcggcgcaag ggtgcgcgcc tacgatccgg tcgccgcccc gcaggcgagt
     8641 aaaatcctgc cggagatcga atacgcggac gacgagtacg cggccgttac gggcgccgac
     8701 gcgctcgtct tcatcaccga gtggaatcag tttcgcgctc tcgacatgga gcgaattcgt
     8761 gggctgatgc gctcgccgaa gatcgccgac ctgcgcaaca tctacgagcc gtcggccatg
     8821 cgcgagatgg gattcgagta cgtcggcgtg ggaaggtagg aacaaacagt gataagtgat
     8881 gagtgataag tgataagaaa gaacgggagc tgttagctct tagctgttag caaagctttt
     8941 ttcttatcac tcatcactca tcactcatca ctcatcacta atatggctct caccggaata
     9001 tatcttgtca caggcggcgc cggcttcatc ggctcgaaca tcgcgggcac gctcgccgaa
     9061 cgcggggcgc gtgttcgtat cgttgacgac ctctcgaccg gccatcagga aaacatcgaa
     9121 gagatcggcg ggcacgtcga tttcatccgg gcgagtgtga ccgactacga cgcgatgagg
     9181 cgcgcgctcg acggcgtgga ggtggtcttc cacgaggcgg ccatcccttc ggtgccgcgc
     9241 tcggtcgata acccgctgga gacgcatcgc gcgtgcgtgg acgggacttt ttcgctgctc
     9301 acggccgcgc gggaggcggg tgtccgccgc gtcgtctacg ccgcttcgag ttcggcttat
     9361 ggtgaacagc ccacgctccc gaaggtggag gacatggccc cgcagcctct cagcccctac
     9421 gccgccgcga agctcgtggg cgaatactac tgtcaggtct ggacacgcac ctacgggctg
     9481 gagaccgtct gcctccgcta cttcaacgtc ttcggcccac ggcaagaccc aagctcacaa
     9541 tactcgggcg tcatctcgcg cttcatagac gcgctctcca ctgctaccca gcccgtcatc
     9601 tacggcgacg gcgagcagtc gcgcgatttc acatatgtct ctaacgccgt ggacgcgaac
     9661 ctgctggccg ccgagagcac ccgcgccgtc ggtcaggtca tcaacgtcgc caacgggcag
     9721 cgcgtcacgc tcaacgagct tctggacagg ctgaagaaga tcacggggcg gacggaggcc
     9781 gaggcggagt ttcgcgagac gcgcgcgggc gatgtgcgcc acagcctcgc ggacatcacg
     9841 cgagcgcggg agttgttagg ttttgagccg caggtggggc tggaagaggg gctgcggcgg
     9901 acgattgact ggtggcggca gagccgcttc gcccaacgtt gaacaactcg ccggggcgcg
     9961 tgacataacg aaaagagccg ggcatctgcg atgaggacgt agggccgaaa gtgtaaaacc
    10021 tttcgcctgc actcgcggac tcccggctct tttttcggtt taagagttat cgcccttaac
    10081 cttcgaccaa ccttctcaag ctctccgtga aaggctgccg ggccacgccg cggtcggtga
    10141 tgatggcggc gatgaattcg ttgggcgtca cgtcgaaggc cgggttctgg atgagaacgc
    10201 cttcgggcgt caggcgctgg tcgcggatgt gtgtgacctc gcgttcgtcg cgctcctcga
    10261 tgggaatctg ttcgccggac gagagtttga gatcgaccgt ggagacgggc gcggcgacgt
    10321 aaaagggaat agcgtgctct cgcgcgagca cggcgaccat ataggtgccg atcttgttgg
    10381 ccgtgtcgcc gttggcggcg atgcggtcgg cgccgacgac gacggcgtca accttcccct
    10441 gcttcatcac gtggcccgcc atgttgtccg taatgaccgt gacgggtatc tcgtctttag
    10501 ccaactccca tgccgtgagc cgcgcgccct gtaggaaggg gcgggtctcg tcggcgatga
    10561 cggcgacgcg cttgccggcg tcgcgcgcgc cgcggatcac gccgagagcg gtgccgtagt
    10621 cgccggcggt ggcgagcgcg cccgcgttgc agtgtgtgag cacggtggcg ccgtcgggga
    10681 tgagttcgcc gccgaagcgg ccgagcgcgc ggttcgactc cacgtcctcc ttaaaaatgg
    10741 cctgcgactc ttcgacgagg cggcgcttga tggcctcggc gtcggcgccc tcggccttcg
    10801 cgcgttggaa acagtcgcgc atccgctcaa tcgcccagaa gaggttgacg gccgtcgggc
    10861 gcgtccggcc cagaaggtcg cagttgtagt cgaaatcgaa ttcgagatcg gcgaccgagg
    10921 tgccgaccga ctgcttggtg ccgagtgcga tgcccatcgc cgccgagacg ccgatggcgg
    10981 gcgcgccgcg gatgaccatc agtttgatgg cctcggccac ctcgtcgcac gaacggaagg
    11041 tcaggtactt ctcctcggtc gggagcagcc gctggtctat catccgcacg ccctcg
//
LOCUS       DASVES010000079         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_910501, whole genome shotgun sequence.
ACCESSION   DASVES010000079 DASVES010000000
VERSION     DASVES010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910501"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF240_02880"
     CDS             <1..703
                     /locus_tag="VF240_02880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEX7174219.1"
                     /translation="LGVGRAPGTDQRTMRALRRTLSNLDEDFPRDVAELLSYFAPPRP
                     GQAVRAVPGAGLRVPVWLLGSSLYSAQLAARMGLPFAFASHFAPDLLMRALQIYRAEF
                     RPSEALASPYAMPCVNVFAADTDEEARRLFTSLQQAFINLRRGQYGRLPPPVDTTEGR
                     WSEVEMAGVEHALRYSAVGSPETVRRWLESFIQETRADEIMLTAQIFDHTARLRSIEI
                     AAGVLGHASGRRASG"
     gene            976..>2087
                     /locus_tag="VF240_02885"
     CDS             976..>2087
                     /locus_tag="VF240_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174220.1"
                     /translation="MFVTRRTLSIIASIILCATSAAAQTAGERKERTATARRPSVTAS
                     ATPAGVNFSTSDGVSIRVEVYSDSGDLVFDSGPRQGGEVGWDLSNLGGAKVADGAYRT
                     RLTVGDHSQYGSVKVEDGQATTVVGGPTPAAATNVDDSIVFSSPNNGQSYARDIRLSD
                     NLGGLRFYASPTMTSTPEGAALQFFGNGSPAFPGQAFLDSGAHNNAAVIFRTAQTGGI
                     IAERMRVTSAGNVGIGTNAPAQKLDVAGNIRVGGAGNGIIFPDGSKQTTAGAGGQMTG
                     TSIVTAVNDPATSGFIIDGRLSPNVARLNGANAWSGANVFGAGLSANNSLVTNVGNPV
                     NAGDAVNKAYTDANFVKFVPGAEQLSVGDANGTAAM"
BASE COUNT          352 a          720 c          660 g          355 t
ORIGIN      
        1 cctcggcgtg gggcgcgcgc cgggaaccga ccagcgcacg atgcgggcgc tgcgccgcac
       61 cctctctaat ttggacgaag acttcccgcg tgacgtggcc gaactcctct cttactttgc
      121 accaccccgc cccggtcagg ccgttagagc cgtgcccggc gcggggctga gagtgcccgt
      181 ctggctgctg ggttcaagcc tctatagcgc gcagctcgcg gcccggatgg gcctgccgtt
      241 cgccttcgcc tcccactttg cgcctgacct cctcatgcgg gcgctccaaa tctacagggc
      301 cgagttccgt ccgtccgagg cactcgcgag tccctatgca atgccgtgcg tcaacgtgtt
      361 cgcggccgac acggacgagg aggcccggcg actgtttacg tcgctccagc aggcgttcat
      421 caaccttcga cgcggccagt acgggaggct tccgccgccg gttgacacta cggaaggccg
      481 ctggtcagaa gtcgagatgg ccggggtcga gcacgcgctc cgctactcgg cggtcggctc
      541 gcccgagacc gtgcggcgct ggctggagtc cttcattcag gagactcgtg ccgacgagat
      601 catgttgacg gcgcaaatct tcgaccacac ggcgcgcctg cgctcaatag agattgccgc
      661 cggggtgctc ggacacgcca gcgggcggcg cgcgtctgga tgaactctcc agagaaccgt
      721 ttcaggaacg gaaatgcaca gataacgccc gcctcttttt ttcacgtatt gaccgaatcc
      781 ctcctgtaac tccccgcatt ttacatagac cagttttcat tttatctccg gccccacagg
      841 ccacgcgtgt gcggcggcgc gcgaacatat atccggtggc ctgatcccaa actgattccg
      901 gttacgcgtt tctgtgtgtc cgggttttta agacccgtta gaccaacagg gattgataca
      961 gggagttgaa atattatgtt cgtcacgcgt cgcacgctat cgattatcgc ttccatcatt
     1021 ctgtgcgcca cctcggccgc ggcgcagacc gccggcgaga ggaaggagcg cactgctact
     1081 gcgcgtcgcc cgtcggtcac ggccagcgcc acgcccgccg gggtgaattt ctccacgagt
     1141 gatggggtga gcatccgcgt cgaagtctat tccgactcgg gcgacctcgt cttcgactcc
     1201 gggccgcgtc agggcggcga ggtcggctgg gacctctcga acctcggcgg cgcgaaggtc
     1261 gccgacggcg cctaccgcac gcgtctcacg gtcggcgacc actcgcagta cggctccgtg
     1321 aaggtcgagg acgggcaggc gaccacggtg gtgggcgggc cgaccccggc cgcggccacg
     1381 aacgttgacg actcgatagt cttctccagc ccgaacaacg ggcagtccta cgccagagac
     1441 atccggctct ccgacaacct cgggggtctg aggttctacg cgtcgccgac gatgacttcg
     1501 acgcccgagg gcgcggcgct ccagttcttc ggcaacggga gtcccgcgtt ccccggtcag
     1561 gcattcctcg actcgggcgc gcacaacaac gcggccgtca tcttccgcac ggcgcagacg
     1621 ggcggcatca tcgcggagcg gatgagggtc acctcggcgg gcaacgtcgg catcgggacg
     1681 aacgccccgg cgcagaagct cgacgtggcg ggcaacatca gggtgggcgg cgccggtaac
     1741 ggcatcatct tccccgacgg ctcgaagcag acgacggccg gcgcgggcgg ccagatgacg
     1801 ggcacgagca tcgtcacggc cgtcaacgac cccgcgacct ccggcttcat tatcgacggc
     1861 cgcctgtcgc cgaacgtggc gcgcctcaac ggcgccaacg cctggtcggg cgcaaacgtc
     1921 ttcggcgcgg gcctctcggc caacaactcg ctcgtcacca acgtcggcaa ccccgtcaac
     1981 gcgggcgacg ccgtcaacaa ggcttacacc gacgcgaact tcgtcaagtt cgtccccggc
     2041 gccgaacagc tctcggtcgg cgacgccaac ggcacggcgg cgatgat
//
LOCUS       DASVES010000080        10996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1467276, whole genome shotgun sequence.
ACCESSION   DASVES010000080 DASVES010000000
VERSION     DASVES010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467276"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..2333)
                     /locus_tag="VF240_02890"
     CDS             complement(138..2333)
                     /locus_tag="VF240_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7174221.1"
                     /translation="MKNRNFLTAGTLCLLLLAALTQNGAVSSFGSSAQSLQTNLTGGQ
                     QTGGTTLTQPDWRSKVDSRVLSALAIAPTEFLIYMKQQADLGGSSSLKTKEEKGRYTY
                     ERLTATAQATQTNVKRTLDQLGVQYQAFWVSNTIAAKGNLAVVQAVAALPEVAAIYPV
                     GRGAIKLPPQANAVASTNAIGGSSSANLAAADLNPEPGLTKVNADDAWALGYEGQGVV
                     VAGADTGVRWTHAALKNQYRGWNGATASHDYNWHDAIHLPNWPAEPLNACNPGGITGA
                     GQPSPAPCDDDELLGGGHGSHTVGSMAGDDGATNRIGMAPQAKWMACRNMSNGVGAIT
                     TYLECMQFFIAPTKVDGSAADPSKAPHVINNSWGCVEGCPPEPNPLRDTLKASRAAGI
                     VYVASAGNDGVGEPGAPGCGTIYHPLARYPEAFTVGSTYHSTDTISEFSSRGPAAADP
                     ENPTAPLYIKPNISAPGSNIRSSLRGSDTEYGSLSGTSMAGPHVAGLVALVISANPAL
                     AGNVDRIEEIIEQSAAKKTTTEGCGGDTATQVPNNTYGWGRIDALAAVQLALADIPTG
                     LVNYALTTLGSVPTASTTQTSRNYSPDSAFDGDRTGGGWEQGGGWNDDTRDLWPDHLD
                     IAFAEGDAKVISEIRVYTLQNNYSQPVVPDENTDASYYGISDFEVQAWNGAQFVTVPG
                     GLITGNTKAMRVITLSTPVTTSAIRVKVNAGRIHFSRIVEVEAFGAPNQ"
     gene            complement(2426..6439)
                     /locus_tag="VF240_02895"
     CDS             complement(2426..6439)
                     /locus_tag="VF240_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7174222.1"
                     /translation="MKFGKKARTATFGALGVSLLLIGLFHYSSVSSAQIGGLPPTGVI
                     WRDVAASRLPVAPAGTRLITPSRFRAVSLDTTALRTLLALAPMEFTQQARTGRIQVSL
                     PTPDGKLSRFLVEESPVMEPALALRHPLIKTYRAQGFDDPAATARFGITPYGFHAIVL
                     SPNGAYYIDPYRRGDTVNHMSYFKRDVPRTEAHNFECQVHTPEGRGDAAAQPLSSESA
                     AAATRPNGSTLRTYRLALAADYEYSDFHSDDQIPDKAEVLAQGIVPAVNRVSGIYERE
                     VAVSLKLVANEELIIFNTPADPYVNEDGTSMLVTNQATIDAVIGPLNYDIGHVASTGG
                     GGVAFLGVVCSVQKAGGVTGLPSPTGDPYYVDYVAHEMGHQFGGEHTFNGSTGSCAGG
                     NRTASNAYEVGSGSTIQAYAGICSPQNLQPNSDPYFHTASYDQILTHITGEADACAVK
                     TSTGNNAPTVDAGPDFQIPAGTAFTLTATGSDPDGDALTYDWEEYDLGPAGDGNTDNG
                     SSPIFRSFNPTPSPSRTFPKLSDQLGNTSSYGEKLPTTTRAMKFRVTVRDNRAGGGGV
                     DYDQMQVNVNATGAPFLVTAPNGGESWPANSAQTVTWDVAGASAAPINASAVDIRLST
                     DGGQTYPHLLASAVPNDGSQSVTMPAVSTSDARVRVAPVGNVFYDISNADFQISPPAL
                     PPVATNDAATTAYQTPVVIPVLANDSDPDGFHLSIASVQSPTGAGGTATIDDNGTPGV
                     TSDDRILYTPPALFSGADQFGYTITDGGTTAAAVVTVTVESFCPAQPTGNFLATFESG
                     NDGFTVLTPANNPASPPWTRVPDPAAHSPAMSFYTDNAAVSGTSAKSDRLISPPQLIS
                     ATSRLIFWHRYLLENDYDGGVLEVSTNGGASYIDITLISAADEFLSGGYNATMGNGPL
                     QFRQAWTSMSPGWPNAMNKVEVDLSALAGQTAIFRWHFRADELNADAAVGWNVDDIQF
                     TNLLVSPPCNEPPFAVSQSVATNEDTPLAVTLTVNQGDDNDPLTYTIESGPAHGALSG
                     TAPNLTYTPAANYSGPDAFAFRASDGANSSNLATVSISVVSVNDPPLVGDDTASVNED
                     TGPNPIDVLRNDTTAPEDGETLTIASVTQGAHGSVVIIDGGSRLTYAPAAGFSGEDAF
                     TYTASDGNGGAATATVRVTVIDVNGPVNYARTVLGSLPFASSTETSRNYSTTSAFDGE
                     RAGANWEQGGGWNDDTRDVWGDHLGVTFGGGGPKTINEIRVYTLQNNYGQPVAPDDNT
                     DASYYGIQNFEVQYMDAGGNWMTVSGGSVTGNTKARRTFVLASPVTATAVRVVVQMGR
                     VHYSRIVELEAYGPPGQ"
     gene            complement(6572..10399)
                     /locus_tag="VF240_02900"
     CDS             complement(6572..10399)
                     /locus_tag="VF240_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX7174223.1"
                     /translation="MKPQKLFRSLALCALALCVLLLGVFHSSPASSVSSAASGGAGQG
                     SAVPERAVNPLAAQTLSRLRSTTGQQIAAHASRRTGVYDFVRVSTGGPTTVLASDNPL
                     GTPESRARAFLSNFGGLVGMNEVERRLAGNSTGAATTSASALQLGRVTTDKLGMTHVR
                     FDQTYMDMKVLGAQVVVHLNAFGVKGFNGNYVPGVNVGKTPKVTAAQARTTALASLRK
                     SPSAANLRAFKTELGVYRDGLLEDRKGRSHLAYAVTVINANGDNVQVWIDAKTGDVLN
                     QISLTPHALYRLVFNKEYDGDDTKAIIEEGNPLKPALPLPPSGAPIPPGYEASALYHY
                     AGQTYNLFLSAFGRDSFDGAGSKMRTVLIYSDDPANDPTKCPNATWNGSTTNYCTEVT
                     ADDVVSHEWGHAYTQYTHDLIYSYQSGALNESYSDIYGETLDLLNGHDAEGGTNNTQP
                     TTYTIQGGVHTPTGGGQRWRIGEDVQGLNIPAAGILRDMWTPVAFSNPDKTSSSLYHC
                     DASDGGGVHANSGIPNHAFAMLVDGKTFNGVTVTGIGLTRALNIYFRAADVYQTPTTN
                     FADHEQALVASCNDLINQPLTVLSTTTGAPVPSADVITAATCQQVQNAMDAVEMDNDV
                     TAQCGFEKLLDPLTPEECDGSAAVFTEDWETGMDGWTVGHQGTGPEFPGADWVLKSTD
                     LPADLDTTAHEGTAIFAINPRIGEPNGGTCQPGGDVTGRFWIDSPPIVVPANGEDLKL
                     AFEHFVQTELEYDGGNVKVSVNGGPFNVIPQDQYIFNPPNFNIGSNNPLTDEFIWNGA
                     NEGELSGSWGTTIANLSNLTNPGDTIKLRFDFGQDACNGNLGWFVDTVRLYSCPFLAP
                     PTLSLGVDYGNPDKDGKYTLNWARGVGAVGPDVLQESSSCGPVLTDDAEEPLVAGQNS
                     KWAGSTQWTSQVNPGDTSTAYFIPAGALQNESLTLKGTVTIPAGASAALTFTTRQGFE
                     DGYDFGKVEVSTNGGANYVEAASYTGPDALHPAEVFEGLRTVDLTQFAGQTIKLRFRV
                     TSDEYSVGAPAGWYIDNINVTASAFQDVATVNDDTYTVTSKPDGNYCYRVSNGYNTGV
                     GIAQSAYSNVVGVSVSNVVCLTNVAASTGGGTATASSTSTSRNYAPSGAIDGDRTGAN
                     WESGGGWNDDTRDLWPDSLEVAFSGSKRVRQINVVTLQDNFQNPSEPTPLMTTSYYGL
                     LDFEVQYQNGEGNWVTLPGGQITNNNLVMRTILLDPLQEVSTTKIRVLVHNARGSFSR
                     IVEVEAIGCDAQP"
BASE COUNT         1608 a         3250 c         4081 g         2057 t
ORIGIN      
        1 gcgccgcagg acgcagggtt cacgcagagg cattgaatca actcaagcct ccgcgccccc
       61 tctcgcgtcc tctgcgcctc tgcggtgaat cacctttcgt aatacgcccg attgagcttc
      121 taccgaacgc gctcgggcta ctggttcggt gcgccgaagg cttcaacctc gacgatgcgc
      181 gagaagtgga tgcggcccgc gttgaccttg acccgaatgg ccgaagttgt gacgggcgtg
      241 ctgagtgtaa tcacacgcat ggccttcgtg ttgccggtga tcaatccgcc cggcacggtg
      301 acgaactgcg cgccgttcca cgcctgcacc tcgaagtcgg agatgccgta gtagctggcg
      361 tccgtgttct cgtcaggcac gacgggctgg ctgtagttgt tctgcaaggt gtagacgcgt
      421 atctcgctga tgaccttcgc gtcgccttcg gcgaaagcga tgtcgaggtg gtcgggccag
      481 aggtcgcgcg tgtcgtcgtt ccagccgccg ccctgctccc agccgccgcc cgtgcggtcg
      541 ccgtcgaagg cgctgtcggg cgagtagttg cggctcgtct gggttgtgga ggccgtgggc
      601 acggagccaa gcgtggtcag cgcatagttg accagtccgg tggggatgtc ggcgagcgcc
      661 agttggacgg cggcgagggc gtcgatgcgg ccccagccgt atgtgttgtt cggcacctgc
      721 gtcgccgtgt cgccgccgca gccttcggtg gtggtcttct tcgcggccga ttgctcgatg
      781 atctcctcga tgcgatcaac gttaccggcc agcgccgggt tggccgagat gacgagggcg
      841 acgaggcccg ccacgtgcgg cccggccatg ctggtgccgc tcaagctgcc atactcggtg
      901 tcgctgccgc gcagtgagga gcggatgttc gagccgggcg cgctgatgtt gggcttgatg
      961 tagagcgggg cggtggggtt ctcggggtcg gcggcggccg ggccgcggct gctgaactcc
     1021 gagatggtgt cggtgctgtg ataggtcgag ccgacggtga acgcctcggg gtagcgcgcc
     1081 agcgggtgat agatcgtgcc gcagccgggt gcgcccggct cgccgacgcc gtcgttgccg
     1141 gccgaggcca cgtagacgat gccggcggcg cggctcgcct tgagcgtgtc ccgcagcggg
     1201 ttcggctccg gcgggcagcc ctccacacag ccccacgaat tgttaatcac gtgcggcgct
     1261 ttcgacgggt cggcggcact gccgtcaacc ttggtcggcg cgatgaaaaa ctgcatgcac
     1321 tcaagatagg tggtgatcgc gcccacgccg ttactcatgt tgcggcaggc catccacttg
     1381 gcctgcgggg ccatgccgat gcggttggtt gcgccgtcgt cgccggccat cgaaccgacg
     1441 gtgtgcgagc cgtggccgcc tcccaaaagc tcgtcgtcgt cgcagggcgc cggcgacggc
     1501 tgcccggcgc cggtgatgcc gccggggttg caggcgttca agggttcggc gggccagttc
     1561 ggaagatgga tggcgtcgtg ccagttgtaa tcgtggctgg cggtggcgcc gttccacccg
     1621 cggtactgat tcttgagcgc cgcgtgcgtc cacctcacgc ccgtgtccgc accggccacg
     1681 acgacgccct gcccctcata acccaaagcc catgcgtcgt cggcgttgac cttcgtcaaa
     1741 cccggctcgg ggttgaggtc ggcagcggcg aggttggccg acgacgagcc gccaatcgcg
     1801 ttcgtcgagg cgactgcgtt ggcctgcggc gggagcttga tggcgccacg gcctacgggg
     1861 tagatggcgg ccacttcagg caacgcggcc acagcctgca ccacggcgag gttgcctttg
     1921 gccgcaatcg tgttcgagac ccagaaggct tgatactgca cgccgagttg gtcgagcgtg
     1981 cgcttcacgt tcgtctgcgt ggcctgcgcg gtcgcggtta gacgctcgta ggtgtagcgc
     2041 cccttctcct ccttggtctt gagcgaggag gagccgccga ggtcggcctg ctgtttcatg
     2101 tagatgagga actcggtcgg ggcgatggcg agcgcgctga gcacgcgcga atcgaccttg
     2161 ctccgccagt ccggctgcgt gagcgtcgtg ccgccggttt gctgtccgcc ggtcaggtta
     2221 gtctgcaagc tctgggcgga cgagccgaaa gacgagacgg ccccgttttg cgtcagcgcc
     2281 gcaagcaaca gcaagcagag tgtgccggcc gtcagaaagt tcctgttctt catgatctct
     2341 cctctcgaag tgtgtgggcc gcggccccgt tgtcagaaac gggaacaccg ctatagcccg
     2401 atgagtccga gcttgccgcc ggcgcctact gccccggcgg gccataggct tccagctcca
     2461 cgatgcgcga gtaatggacg cgtcccattt gaacgaccac gcggacggcc gtcgccgtca
     2521 ccggcgaggc gagcacgaac gtgcgccgcg ccttcgtgtt gccggtgacc gaaccgcccg
     2581 acacggtcat ccagttgccg cccgcgtcca tgtactgcac ctcgaaattc tggatgccgt
     2641 agtagctggc gtccgtgttg tcgtcgggcg cgacgggctg gccgtagttg ttctgcaagg
     2701 tgtagacgcg tatctcgttg atggtcttgg ggccgccgcc gccaaaggtg acgccgaggt
     2761 ggtcgcccca cacgtcgcgc gtgtcgtcgt tccagccgcc gccctgctcc cagttggccc
     2821 cggcgcgctc gccgtcgaag gcgctcgtcg tcgagtagtt gcggctcgtc tcggtcgagg
     2881 aggcgaaagg cagcgagccg agcacggtgc gggcgtagtt gacggggccg ttcacgtcga
     2941 tgaccgtgac ccggacggtg gcggtcgccg cgccgccgtt gccgtcggag gcggtgtagg
     3001 tgaaggcgtc ctcgccgctg aagccggcgg cgggcgcgta ggtgagacgc gagccgccgt
     3061 cgatgatgac gaccgagccg tgcgccccct gcgtgaccga tgcgatggtc agtgtctcgc
     3121 cgtcctcggg cgcggtcgtg tcgttgcgga gcacgtcgat ggggttcggc ccggtgtctt
     3181 cgttgacgga ggcggtgtcg tcgccgacga gcggcgggtc gttgacggag acgacgctga
     3241 tcgacaccgt tgcgaggttc gaggaattcg cgccgtcgct cgcgcggaag gcgaaggcgt
     3301 cggggccgct gtagttggcg gccggcgtgt aggtcagatt cggggccgtg cccgacagcg
     3361 cgccgtgcgc ggggccggac tcgatggtgt aggtcagagg gtcgttgtcg tcgccctgat
     3421 tgacggtgag cgtgacggcg agcggtgtgt cctcgttggt cgcgaccgac tggctcaccg
     3481 cgaagggcgg ctcgttgcat ggcggcgaga cgaggaggtt ggtgaactgg atgtcatcga
     3541 cgttccaacc gacggccgcg tcggcgttga gttcgtcggc gcggaagtgc cagcggaaga
     3601 tggccgtctg gccggcgagc gcgctcaggt caacctcgac cttgttcatg gcgttgggcc
     3661 agccgggtga catactcgtc cacgcctgcc ggaactggag cgggccgttg cccatcgtgg
     3721 cgttgtagcc gccggagaga aattcgtcgg cggcgctgat gagagtgatg tcgatgtagc
     3781 tcgcgccgcc gttagtggag acttcgagga cgccgccgtc gtagtcgttc tcaagcaggt
     3841 agcggtgcca gaagatgaga cggctcgtcg cgctgatgag ttgtggcggc gagatgagtc
     3901 ggtcggactt cgccgacgtg ccgctgacgg cggcgttgtc ggtgtagaac gacatcgcgg
     3961 ggctgtgggc ggccgggtcg ggcacgcgcg tccacggcgg gctggccggg ttgttggccg
     4021 gcgtcagcac ggtgaagccg tcgttgcccg actcgaaggt ggcgaggaag ttgcccgtgg
     4081 gctgcgcggg gcagaaggac tcgacggtga cggtgacgac cgccgcggcg gtcgtgccgc
     4141 cgtcggtgat ggtgtagccg aactggtctg cgccggagaa gagcgccggc ggcgtgtaga
     4201 ggatgcggtc gtcggaggta acgccgggcg tgccgttgtc gtcgatggtg gccgtgccgc
     4261 cggctcccgt cggcgattgc acggaggcga tggagagatg gaagccgtcg gggtcgctgt
     4321 cgttggcgag cacggggatg acgaccggag tttgatacgc ggtggtcgcc gcgtcattcg
     4381 tcgcgacggg cggcagcgcg ggcgggctga tctggaagtc ggcgttcgag atgtcgtaga
     4441 agacgttgcc cacgggcgcg acgcggacgc gcgcgtccga cgtggagacg gccggcatgg
     4501 tcacgctctg cgagccgtcg ttggggacgg ccgaggcgag caggtgcggg taggtctggc
     4561 cgccgtcggt cgagagacgg atgtccacgg ccgacgcatt gatgggggcg gcgctggcgc
     4621 cggccacgtc ccacgttacg gtctgagcag agttcgcggg ccacgactcg ccgccgttgg
     4681 gcgcggtgac gagaaacggc gcgccggtcg cgttgacgtt gacctgcatc tggtcgtaat
     4741 cgacgccgcc gccgcccgcg cggttgtcgc ggacggtgac gcgaaacttc atggcgcgcg
     4801 tcgtcgtcgg cagcttctcg ccgtagctcg aagtgttgcc cagttggtcg ctcaatttcg
     4861 ggaaggtgcg cgaggggctg ggcgtcgggt tgaaggaacg gaagattggc gacgagccgt
     4921 tgtcggtgtt gccgtcgccg gccgggccaa ggtcgtactc ctcccagtcg taggtgagcg
     4981 cgtcgccatc ggggtcgctg cccgtggccg tcagggtgaa ggcggtgcca gccggaatct
     5041 gaaagtcggg gccggcgtcc accgtgggcg cgttgttgcc cgtcgaggtc ttgacggcgc
     5101 aggcgtcggc ctcgccggtg atgtgcgtga gaatctggtc gtagctggcg gtgtggaaat
     5161 atgggtcgct gttgggctgg aggttttgcg gcgagcagat gccggcgtaa gcctgaatgg
     5221 tcgagccgct gccgacctcg taggcgttcg aggcggtgcg gttgccgccc gcgcaggagc
     5281 cggtcgaacc gttgaaggtg tgctcgccgc cgaactggtg gcccatctcg tgagccacgt
     5341 aatcgacgta gtaggggtcg cccgtgggtg acggcagacc cgtcacgccg ccggccttct
     5401 ggacagagca gacgacgccg aggaaggcga cgccgccgcc gcccgtcgag gcgacgtggc
     5461 cgatgtcgta gttgagcggg ccgatgacgg cgtcgatggt cgcctgatta gtgacgagca
     5521 tcgaggtgcc atcctcgttg acgtatgggt cggccggcgt gttgaagatg atcaactcct
     5581 cgttggcgac gagcttgagg ctgaccgcga cctcgcgctc gtagatgccg gagacgcggt
     5641 tgacggccgg gacgatgccc tgcgcgagca cctcggcctt gtcgggtatc tggtcgtcgc
     5701 tgtggaaatc ggagtactcg taatcggcgg cgagggcgag gcggtaggtg cgaagcgtgg
     5761 agccgttggg acgtgtggcg gcggctgccg actcggacga gagcggctgc gccgccgcgt
     5821 cgccgcgacc ctcgggcgtg tgtacctgac actcgaaatt gtgcgcctcg gtgcgcggca
     5881 cgtcgcgctt gaagtagctc atgtggttga cggtgtcgcc gcggcggtag gggtcgatgt
     5941 agtaagcgcc attcggggag agcacgatgg cgtggaagcc gtagggcgtg atgccgaagc
     6001 gggcggtggc cgccgggtcg tcgaaccctt gcgcgcggta ggtcttaatc agcgggtggc
     6061 gcagcgcgag cgccggctcc atcaccggcg actcctcgac gagaaagcgc gagagcttgc
     6121 cgtcgggcgt gggcagggag acctgtatgc ggcccgttcg cgcttgctgt gtgaactcca
     6181 tcggcgcgag cgcgaggagc gtgcgtagcg ccgtcgtgtc gagcgagacg gcgcggaagc
     6241 gactcggtgt gatgagacgc gtgccggcgg gggcgacggg aagacgcgac gcggccacgt
     6301 cgcgccagat gacgcccgtg gggggaagcc cgccgatttg tgcggaggag accgaagagt
     6361 agtggaacaa tccgatcagc agtagcgaaa cgccgagagc gccgaacgtg gccgttcgcg
     6421 cctttttacc gaacttcatg acgggtctcc tcagcatggt taggggtttc gatatttcta
     6481 ctggccgtgc ggcggattcg tccgcggcga gtgctgaaag ttttcacgcc gcccggctcg
     6541 tcgggaaccg ggcggcgcga atgttttcgc tttacggttg cgcgtcgcag ccgatggctt
     6601 ccacctcgac gatgcgcgag aaggagccgc gggcgttgtg gacgagcacg cgtatcttcg
     6661 tggtgctcac ctcctgaagc gggtcgagca ggatggttct catcacgagg ttgttgttcg
     6721 tgatctggcc gccgggcagc gtgacccagt tgccttcccc gttctggtat tgcacctcga
     6781 agtcgagcag gccgtagtag gaggtcgtca tcagcggcgt cggctcgctc ggattttgga
     6841 agttgtcctg aagcgtgacg acgttgatct gccggacgcg cttgctgcca ctgaaggcca
     6901 cttcgagcga gtcgggccaa aggtctcgcg tgtcgtcgtt ccagccgccg cccgactccc
     6961 agttggcgcc cgtgcggtcg ccgtcaatcg cccccgaggg cgcgtagttg cggctggtcg
     7021 aagtggacga ggccgtggcc gtgccgccgc ccgtcgaggc cgcgacgttc gtcaggcaga
     7081 cgacgttcga gaccgagacg cctacgacgt tgctgtaggc gctctgcgcg attccgacgc
     7141 ccgtgttgta gccgttgctg acgcggtagc agtagttgcc gtcgggtttc gacgtgacgg
     7201 tgtaggtgtc gtcgttgacg gtcgccacgt cctgaaacgc gctggccgtg acgttgatgt
     7261 tgtcgatgta ccagccggcg ggcgcgccga cgctgtactc gtcgctcgtc acgcggaagc
     7321 gcagcttgat ggtctgcccg gcgaactgcg tgagatcgac cgtgcgcaag ccctcgaaga
     7381 cttcggccgg gtgaagggcg tcggggccgg tgtaggaggc cgcctcgacg tagttcgcgc
     7441 cgccgttggt gctcacttcg accttaccga aatcgtagcc gtcctcgaag ccctgccggg
     7501 tggtgaaggt cagcgcggcc gacgcgccgg cgggaatcgt gacggttccc ttcaacgtca
     7561 gcgactcgtt ctggagcgcg ccggcgggga tgaagtaggc cgtgctcgtg tcgccggggt
     7621 tgacctgcga agtccactgt gtgcttccgg cccacttcga gttctggccg gcgaccagag
     7681 gctcctcggc gtcgtcggtc aagacggggc cgcagctcga cgattcctgc aacacgtcgg
     7741 ggccgacggc tccgacgccg cgcgcccagt tgagcgtgta cttgccgtcc ttgtcggggt
     7801 tgccgtaatc gacgccgagc gaaagggtcg gcggcgcgag gaacgggcaa ctgtagagac
     7861 gcacggtatc gacgaaccag ccgaggttgc cgttgcaggc gtcctgtccg aagtcgaagc
     7921 gaagcttgat ggtgtcgccg gggttggtca ggttcgagag gttggcgatg gtcgtgcccc
     7981 acgagccgga gagttcgccc tcgttggcgc cgttccagat gaactcgtcg gtcagcgggt
     8041 tgttgctgcc gatgttgaag ttcggcggat tgaagatgta ctgatcctgc gggatgacgt
     8101 tgaaggggcc gccgttgacg gagaccttga cgttgccgcc gtcgtattca agctcggtct
     8161 ggacgaagtg ctcgaaggcg agctttaagt cttcaccgtt ggccgggacg acaatcgggg
     8221 gactgtcgat ccagaagcgg cccgtcacgt cgccgcccgg ctggcaggtg ccgccgttcg
     8281 gctcgccgat gcgcgggttg atggcgaaga tggccgtgcc ttcgtgcgcc gtggtgtcga
     8341 ggtcggcggg caggtctgtt gacttgagca cccagtcggc gccggggaac tccggccccg
     8401 tgccctgatg tccgaccgtc cagccgtcca tgcccgtctc ccagtcttcg gtgaagacgg
     8461 ccgcggagcc gtcgcactct tcgggagtga gcgggtcgag cagcttctcg aagccgcact
     8521 gcgcggtcac gtcgttgtcc atctcgacgg cgtccatcgc gttctgcacc tgctggcagg
     8581 tggccgcggt gattacgtcg gcggatggga cgggcgctcc cgtcgtcgtc gagagcacgg
     8641 tgagcggctg gttgatgaga tcgttgcagg aggcgacgag cgcctgctcg tggtcggcga
     8701 agttcgtggt gggcgtctgg tagacatcgg ccgcgcggaa gtagatgttg agcgcgcggg
     8761 tcaggccgat gccggtgacg gtgacgccgt tgaaggtctt gccgtcaacg agcatggcga
     8821 aggcgtgatt cgggatgccg gagttggcgt ggacgccgcc gccgtcgctc gcgtcgcagt
     8881 gatagagcga cgaagaagtc ttgtccgggt tgctgaaggc gaccggcgtc cacatgtcgc
     8941 gcaggatgcc ggcggccggg atgttcaagc cctgcacgtc ctcgccgatg cgccagcgct
     9001 ggccgccgcc cgtcggcgtg tgaacgccgc cctgaatggt gtaggtggtg ggctgcgtgt
     9061 tgttggttcc gccctcggcg tcgtggccgt tcaggaggtc gagcgtctcg ccgtagatgt
     9121 cggagtaaga ctcattgagc gcgccggact ggtaagagta gatgaggtca tgcgtgtact
     9181 gcgtgtaggc gtggccccac tcgtgcgaga ccacgtcgtc ggccgtcact tcggtgcagt
     9241 agttggtggt cgagccgttc caggtggcgt tgggacactt ggtcgggtcg ttggcggggt
     9301 cgtccgagta gatgaggacg gtgcgcatct tgctgcccgc gccgtcgaag gaatcgcgtc
     9361 cgaaggcgga gaggaagagg ttgtaggtct gcccggcgta gtgatagagg gcgctggcct
     9421 catagcccgg agggatgggt gcgccggagg gcggcagggg caaggccggc ttgagggggt
     9481 tgccctcttc gatgatcgcc ttggtgtcgt cgccgtcata ctctttgttg aagacgaggc
     9541 ggtagagcgc gtgcggcgtc agagaaatct ggttgagcac gtcgcccgtt ttggcgtcaa
     9601 tccacacctg cacgttgtcg ccgttcgcgt tgatgaccgt cacggcatag gcgaggtggc
     9661 tgcggccctt gcggtcttcg agcagcccgt cgcggtagac gcccagctcc gtcttgaacg
     9721 cgcgcaggtt ggcggccgac gggcttttac gcagggaggc gagcgccgtc gtccgggcct
     9781 gtgcggccgt gaccttcggg gtcttgccga cgttgacgcc ggggacgtag ttgccgttga
     9841 agcccttgac gccgaaggcg ttcaggtgga cgacgacctg tgcgccgagc accttcatgt
     9901 ccatgtaggt ctgatcgaac ctgacgtgcg tcatgccgag cttgtcggtg gtgacgcgcc
     9961 cgagttggag cgcgctggcg gaggtggtcg ccgcgccggt cgagtttccg gccagacggc
    10021 gctcgacctc gttcatgccg acgaggccgc cgaagttgga gaggaaggcg cgggcgcgcg
    10081 actcgggcgt gccgagcggg ttgtcggagg cgagcacggt cgtcgggccg ccagtggaga
    10141 cgcgcacgaa gtcgtagacg ccggtgcggc gcgaggcgtg ggcggcgatc tgctggcccg
    10201 tcgtgctgcg gaggcgcgaa agagtttgcg cggccagcgg gttgacggcc cgttcgggca
    10261 cggcgcttcc ctgcccggcc ccgccgctcg cggcgctgct gaccgaagac gcgggcgagg
    10321 aatggaagac gcccaacagg aggacgcaca gggccaacgc gcacagggcc aaacttcgga
    10381 acaatttctg gggcttcatc gggtctcctt cttctttcgc ggctcgactg gcggccgact
    10441 tctaaacttt tacttcgacg acgcctcgca ctttaagagg aggcggttga gacatacttg
    10501 accggcgttt ttgttggata aaagactacc ggaaggtggt tgaggagaac ttgaggcggg
    10561 gcggaagacg cgcgagccgc cggaggcggc gatagaatgt agcccacggc gcgagccgtg
    10621 gggacgcgcc ggataaaagc agtgaagccc cggaggggcg aaagaatctc gacgtgcgtc
    10681 ccacgccaac actgagattc tttcgcccct ccggggctta cttgttgttt ttcttaacct
    10741 taccccggtc tcacgaccgg ggctacaatc ttccgccgcc tccggcggct cgttgtcgtc
    10801 gaatctactt cgcacttatt caactgaata aaaccactct agtgcatctt gaacggccca
    10861 cccggcttct ccgcctccaa atccgggcgg gccgaatcct gaacgacctc gtgcatctcc
    10921 aacacgttcg ctctggagaa cgcctctttc ggcaaggtgc cggactgtgc gtgccccttc
    10981 gtgaaagcgt cggagc
//
LOCUS       DASVES010000081         7862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_436696, whole genome shotgun sequence.
ACCESSION   DASVES010000081 DASVES010000000
VERSION     DASVES010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436696"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1928
                     /gene="aspS"
                     /locus_tag="VF240_02905"
     CDS             108..1928
                     /gene="aspS"
                     /locus_tag="VF240_02905"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX7174224.1"
                     /translation="MEREVTLDHLGDLERTHSCGALRAEHAGSDVLLMGWVARRRDFG
                     PLTFFDLRDREGITQVVFNEETAPTAHARAKAPRGEYVIAVRGQVVLRDEAQRNQKIA
                     TGEVEVQARDIWILNDARTPPFQIESATPESIAAEDVRLKYRYLDLRRPALQQNIRMR
                     HRVLSEIRRYMDEQGFTEIETPVLIKSTPEGARDYVVPSRTFPGNFYALPQSPQLFKQ
                     LCMIAGLDKYYQIARCFRDEDLRADRQPEFTQLDVEFSFASLEQVYTLVEGLFARVFR
                     LIGVELETPFPRYPYSEAMRRFGSDKPDTRFGMELQDLSAALEGTTFPPFAAALEAGG
                     EVKGIVVRGGAHLSRKVLDELQEYVKRYGALALAWVKLGDELSSSLLKVLGEETVARV
                     ARDAGAERGDAVLIVAGRPKVVAASLGALRNEMARREKLIPEGVYRPLWVTEFPMFEY
                     HEEDGRFYAMHHPFTSPLDEDLALLERAVEGGETELLGSLRSKAYDPVINGVEMASGS
                     IRIHRRDVQRLIFKALGMTEEEARSRFGFFMDALEYGTPPHGGIAPGIDRLMMVVLGT
                     DNMRDVIAFPKTASAADLMLDAPGPVDARLLEELHIKVVE"
     gene            1964..2377
                     /locus_tag="VF240_02910"
     CDS             1964..2377
                     /locus_tag="VF240_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX7174225.1"
                     /translation="MEILRAHVDEMFAHARASAPAECCGLVGGRGASALSVYPLRNVA
                     ADAAVAYEAAPVELFAAQRLMRERGEQLLAVYHSHPRSEDPRPSATDVRLAFYPEAVY
                     FIIGFTRGGEGILGAFRIHEAEGRWERAEFRVVED"
     gene            2447..3166
                     /locus_tag="VF240_02915"
     CDS             2447..3166
                     /locus_tag="VF240_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174226.1"
                     /translation="MTEKRNKYDTDPLDPDYVRRTEEFGSAATRDVSRTPAEEARRDP
                     AAEEPTRRFYEPPPAAASAEENFSASYPSVFVPPPSYQTPPPQHAHTAFGGAPYQPHP
                     QAPIAPQAHGSVSKPSQRPIEKLGIPENMALALPYAPFFIGLVAAIVELLMVPRTETR
                     TRFHASQGLALQLVVLAGTLVFNVVEAITDIGLGGTLFWLASTIFLIVSAFRVWEGRP
                     HHIAPLDDLTQKINQKFEPRK"
     gene            3346..5706
                     /locus_tag="VF240_02920"
     CDS             3346..5706
                     /locus_tag="VF240_02920"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX7174227.1"
                     /translation="MQTIRSIAGKLPAPLVALALLVASLAPATAAQRSSRRGARRGPS
                     SRAAAPPRVSGNSDERLRQIADEYLRGHFAFRPSEATAAGLHEYDTQLDSRSPEAVAR
                     EVRRLRAALAELQRVPEWRLTPEARYDYLVLQSHARAQLLELEEVRMWRRDPNVYNRL
                     AAEAIDNVLKREYAPGEQRLDAMMARARQIPRLLSEARANVENPPRLYTETAIAEARG
                     SLDFFARVVPQLVERAGGGRLTAARRAEIDATNESVVAAVRSFVEWLETDLLARSDGD
                     FALGAEVYRRKLLYEEMIDTPLSALLRAGEEELRRTQEAMRAVAEEVAPGRGLTFALQ
                     TLGRERPTAAGLAGDARAELGRLRAFVRAEGIMTPPTRERLAVAETPEYARALSFSSL
                     SAPGPFERASDDAFYYVTPPDPARGVVLAEEHLSHFNRYALQIISIHETYPGHYYQQL
                     SLARTTSRLRTALGSASFVEGWAHYCEQMMLDEGFGGNNPKLRLAQLHAAALGLCRYL
                     AGIRLHTEGMSYEQAVEFFGREGHLPRSSAEREARRGALDPTYVVHTLGKMEITRLRE
                     EWRRQMGDSFRLGEFHDRLLSYGMPPVRILRMAMLGDAGNSVSGVRAGNPVGGGGGVD
                     LSDAAPAEQSVPVDFSVIASGQMSGYEGARRVELVTSEAEWRRVWETIGEGRPAPEVN
                     FDTRAVVVAYQGRQSTGGHGIEIAGVRRVGTVLAIKVNERRPASGDITTQVLTSPYVA
                     ATIARPPSGAHVRFDEPAAASPKALERKGVRKSRARTPRRPRRRGR"
     gene            6001..7446
                     /locus_tag="VF240_02925"
     CDS             6001..7446
                     /locus_tag="VF240_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7174228.1"
                     /translation="MLSNNRPVRFVAVWAMALMMLAAPLAALAQTQVVMPKNKYNVND
                     DVKLGQQAAQQAEQQLPVLRDESVESYVARVGQRLVSAIPPQFQQPQFQYRFRVIDAR
                     DINAFALPGGPMYVNRGMIEAARTEGEMAGVMAHEISHVALRHATAQATETQKFQIGS
                     VLGQIAGAVIGGPAGAVIGTASQIGFGAGALKYSRKYETQADILGSQIMARAGYDPRD
                     LANMFRTIEGQGGGARGPEWMSSHPNPGNRYERINQEAAMLRVSNPNRDMREFSQIQA
                     RLRGQPRARSMEEIARSGQTNPQGTRYPDNRYPDNRDTRYPSGERVAYPSTRLRAYQG
                     SFFRLGVPDNWRELGGQQNAVTFAPDGAYSQQGEFTHGMMVGIAPAQSRDLRGASDEF
                     LQGLLQGGNSYLRPQSRGYQRASIGGRSALAMPLTGRSPLTGQNEYVVVYTTQLRDGN
                     VFYLVTVAPQSDYNSFERAFRSVLSSVQLND"
     gene            7550..7798
                     /locus_tag="VF240_02930"
     CDS             7550..7798
                     /locus_tag="VF240_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174229.1"
                     /translation="MARGWESKSVADQMDAAGAAPAREDSRYEVSPAVRERQTRLRWL
                     RLSHARTSEQLKRATNDAHRQMLGRAIRALEREMEDLV"
BASE COUNT         1301 a         2676 c         2645 g         1240 t
ORIGIN      
        1 aaggacatgc ggacgggtga gcagcgggcc gtcggcgcgg gcgaagtggg cgcggcggtg
       61 cgcgaaggaa aatacacacg cgagagttag gtcaggacgt ttattgcatg gaaagagaag
      121 tcacactcga tcacctcgga gaccttgagc gcacgcactc gtgcggcgcg ctgcgggcgg
      181 agcacgccgg ctcggacgtg ttgctgatgg gctgggtggc ccgccgccgc gacttcggcc
      241 cgctcacctt ttttgatttg cgcgaccgcg aaggaatcac gcaggtcgtc ttcaacgaag
      301 agaccgcgcc cacggcgcac gcccgcgcga aggcgccgcg cggcgagtac gtcatcgccg
      361 tgcgcgggca agtagtgctg cgcgacgagg cgcagcgcaa tcagaagatc gccaccggcg
      421 aggtggaggt gcaggcgcgc gacatctgga ttttgaacga cgcgcgcacg ccgcccttcc
      481 agatcgagtc ggccacgccc gaatccatcg ccgccgagga cgtgcgtctc aaatatcgct
      541 atctcgattt gcgccgcccc gcgctccagc aaaacatcag gatgcgccac cgcgtgctct
      601 ccgagattcg ccgctacatg gacgagcagg gcttcacgga aatcgagacg cccgtcctca
      661 tcaagtccac ccccgagggc gcgcgcgatt acgtcgtgcc gtcgcgcact tttccgggaa
      721 acttctacgc gctgccgcag tcgccgcagc tcttcaaaca gctctgcatg atcgccgggt
      781 tagataagta ctaccagatc gcccgctgct tccgcgacga agacctccgc gccgaccgcc
      841 agcccgagtt cacgcagctc gacgtggagt tcagcttcgc ctcgctcgaa caggtctaca
      901 cgctcgtcga gggactgttc gcgcgcgtct tccgcctcat cggggtggag ctggagacgc
      961 cgtttccccg ctacccttac agcgaggcga tgcgccgctt cgggtcggac aagcccgaca
     1021 cccgtttcgg gatggagttg caagacctct cggcggcgct ggaaggaact acgttcccgc
     1081 cgttcgcggc ggcgctcgaa gcgggcggcg aggtaaaggg catcgtcgtg cgcggcggcg
     1141 cgcacctgtc gcgcaaggtg ctcgacgaat tgcaggagta cgtcaaacgc tacggcgcgc
     1201 tcgcgctcgc gtgggtcaag ctcggcgacg agctatcgtc ctcgctgctg aaggtgttgg
     1261 gcgaagagac tgtggcgcgt gtcgcgcgag acgcgggcgc ggagcgcggc gacgcggttc
     1321 tgatcgtcgc cgggcggccg aaggtggtcg ccgcgtctct gggcgcgctc cgcaacgaga
     1381 tggcgcggcg cgagaagttg atacccgagg gcgtctaccg gccgctgtgg gtcacggagt
     1441 ttccgatgtt cgagtatcac gaggaggacg ggcgcttcta cgcgatgcac cacccgttca
     1501 cctcgccgct cgacgaagac ctggccttgc ttgagcgcgc cgtcgagggc ggcgagacgg
     1561 aactgctggg cagccttcgc tcgaaggctt acgatcctgt catcaacggc gtcgagatgg
     1621 cgtcaggctc gattcgtatt caccgccgcg acgtgcagcg actcatcttc aaggcgctgg
     1681 ggatgacgga ggaagaggcc cgctcgcgct tcgggttctt catggacgct ttggagtacg
     1741 gcaccccgcc gcacggcggg atcgcgccgg gcatcgaccg cctgatgatg gtcgtgcttg
     1801 gcaccgacaa catgcgcgac gtgatcgcct tccccaagac cgcctcggcc gccgacctca
     1861 tgctcgacgc gcccggcccc gtggacgcgc gtctgctcga agagttgcac atcaaggtcg
     1921 tcgagtagga agcgggttcg cctgcgcggg ggcgcgtgtg gtcatggaga ttctgcgcgc
     1981 acacgtcgat gagatgttcg ctcacgcgcg cgcgtcggcg cccgcggagt gctgcgggct
     2041 ggtcggcggg cgaggcgcgt cggccctgag cgtttatccg ctgcgtaacg tcgcggccga
     2101 tgcggcggtc gcctacgagg ccgcgcccgt cgagttgttc gccgcgcaga ggctcatgcg
     2161 cgagcgcggc gagcagttgt tggccgtcta ccattcgcac ccgcgctccg aagaccctcg
     2221 gccttccgcg acagacgtgc ggctcgcctt ttaccccgag gccgtttact tcataatcgg
     2281 gttcacgcgc ggcggcgaag gaattctcgg cgccttccgc atccacgagg ccgaggggcg
     2341 ttgggagcgc gccgagttcc gcgtcgtcga agactgaggg gtgaacctgc gggcgcgctt
     2401 cttcgtaaaa cgtctggtga aacaaacaac cttgccggtg aaagcgatga cggagaagag
     2461 gaacaagtac gacacggacc cgctcgaccc cgactacgtg cgccgcacgg aggagttcgg
     2521 atcggcggcc acgcgcgacg tgtcgcgcac gccggccgag gaggcgcgac gcgaccccgc
     2581 cgccgaggag ccgacgcgcc gcttctacga gccgccgccc gccgccgcgt ccgccgagga
     2641 aaacttctcg gcctcgtacc cgtccgtctt cgttccgccg cccagctacc agacgccgcc
     2701 gccgcagcac gcgcacaccg ccttcggggg cgcgccctac cagccgcacc cgcaggcgcc
     2761 catcgcgccg caagcgcacg gctcagtgtc gaagccctcg caacggccca tcgagaagct
     2821 gggcatcccg gagaacatgg cgttggctct gccctacgcg cctttcttca tcggcctcgt
     2881 cgccgccatc gtcgagctgc tgatggtgcc gcgcacggag acgcgcacgc gctttcacgc
     2941 ctcgcagggc ctcgcgctcc agctcgtcgt actggccggc acgctcgtct tcaacgtcgt
     3001 cgaggcgatc acggacatcg gcctcggcgg cacactcttc tggctcgcct cgaccatctt
     3061 cctcatcgtc tcggcgtttc gcgtctggga gggtcgcccg caccacatcg cgccgctcga
     3121 cgacctcaca cagaagatca accagaagtt cgagccgcgc aagtaattaa agccgtaaat
     3181 cgtgaatcgt aaatcgtgaa tcgtggttgg acggcagttc aacgcgggtg gtgaatcgca
     3241 ggccggaggg cgggcaatcg attcacgatt cacgatttac gattcacggc ttttacgcta
     3301 ctctcataat tagttcgtca gccggcgctc tcctacttaa agcttttgca aacgattcgc
     3361 tccatagcag ggaagctccc cgcgccgctc gtcgcgctcg cgctcctcgt ggcgtcgctc
     3421 gcgcccgcga cggccgcaca gcgttcgtcg cgtcgcggtg cgcgccgtgg cccgtcgtcg
     3481 cgcgccgccg cgccgccgcg cgtgagcggg aatagcgacg aacgcctgcg ccagatcgcc
     3541 gacgagtacc tgcgcggcca cttcgccttc cgcccctcag aggcgacggc cgcgggcctg
     3601 cacgagtacg acacgcagct cgactcgcgc tcacccgagg cggtcgcgcg cgaggtgcgc
     3661 cgcctgcgcg cggccctcgc cgaactccag cgcgtccccg agtggaggct cacgcccgag
     3721 gcgcgctacg actacctcgt cctccagtcg cacgcccgcg cgcagctcct cgaactcgaa
     3781 gaggtgcgga tgtggcggcg cgacccgaac gtctacaacc gcctcgcggc cgaagctatc
     3841 gacaacgtgc tcaagcgcga gtacgcgccc ggcgagcagc gcctcgacgc gatgatggcg
     3901 cgcgcccgcc agattccccg cctgctctcc gaggctcgcg ccaatgtgga gaacccgccg
     3961 cgcctttaca ccgagacggc catcgcggag gcccgcggca gtctcgactt cttcgcgcgc
     4021 gtcgtgccgc agctcgtcga gcgcgccggc ggcgggcgat tgacggccgc gcgccgcgcc
     4081 gaaatcgacg ccaccaacga gtcggtcgtc gcggccgtcc gctccttcgt cgagtggctg
     4141 gagaccgacc tgctggcgcg ctcggacggc gacttcgcgc tgggcgcaga ggtctatcgc
     4201 cgcaagctcc tctacgagga gatgatcgac acgccgctct ccgcgctcct gcgcgcgggc
     4261 gaggaagagt tgcggcgcac gcaggaggcg atgcgcgccg tggccgagga ggtcgcgccg
     4321 ggacgtggcc tgacgttcgc cctgcaaacg ctcgggcgcg agcgcccgac ggccgcgggc
     4381 ctcgccggcg acgcgcgcgc cgaactcggt cgcctgcgcg ccttcgtccg cgccgagggg
     4441 atcatgactc cgcccacgcg cgagcgtctg gccgtcgccg agacgccgga gtatgcgcgc
     4501 gccctgagct tctcgtcgtt gtccgcgccg gggccgttcg agcgcgcctc ggacgacgcc
     4561 ttctactacg tcacgccgcc cgacccggcg cgtggcgtgg tgttggcgga agaacatctc
     4621 agtcacttca accgctacgc gctccagatc atctccattc acgagaccta ccccggccac
     4681 tactaccaac agctctcgct tgcgcgcacg acctcgcgcc tgcgcactgc gctcggctcc
     4741 gcgagcttcg tcgagggctg ggcgcactac tgcgagcaga tgatgctcga cgaggggttc
     4801 ggcggcaaca acccgaagct gcggctcgca cagctccacg cggccgcgtt gggcctgtgc
     4861 cgctacctcg ccggcatccg cctgcacacc gaagggatga gctacgagca ggccgtcgag
     4921 tttttcgggc gcgagggcca tctgccgcgc tcgtcggcgg agcgcgaggc ccggcgcggg
     4981 gcgctcgacc cgacttacgt cgtccacacg ctcggcaaga tggagatcac gcgtctgcgc
     5041 gaggagtggc ggcggcagat gggcgacagc tttcgcctcg gcgagttcca cgaccgcctc
     5101 ctgagctacg gcatgccgcc cgtcaggatt ctacgcatgg cgatgctcgg cgatgcgggc
     5161 aacagtgtca gcggcgtgcg cgcgggcaac ccggtcggcg gcggcggcgg cgttgacttg
     5221 tcggacgccg cgccggccga gcagtctgtg cccgtcgatt tctccgtgat cgcttcgggg
     5281 cagatgagcg gctacgaggg tgcgcgccgg gtcgagcttg ttacgagcga ggctgagtgg
     5341 aggcgcgtgt gggagacgat tggcgaggga cggcccgcgc ccgaggtcaa cttcgacacg
     5401 cgcgccgtcg tcgtcgccta tcaggggcgg cagtcaacgg gcggccacgg catcgagatc
     5461 gcgggcgtgc gccgcgttgg cactgtgctt gctatcaagg tcaacgagcg gcgcccggcg
     5521 tctggagaca ttacaacgca ggtgctcact tcgccctacg tcgccgccac gatagcgcgc
     5581 ccgccctcgg gcgctcacgt caggttcgac gagccggccg cggccagccc gaaagcgttg
     5641 gaacgtaaag gggttaggaa atcacgcgcc cggacgccgc gccgcccgcg ccggcgtgga
     5701 cgctaattga cggcgaaccg ccgtgttttg aaattcatcg aaatgtagcc aaaaggtacg
     5761 gcccggcgta atcgtccggg ccgtgggtta gcgaaataat tacttctctc ctcgcgcata
     5821 agtccccgta aggcacgggg cgcttttgat taaggtgaag cagggggaga gatcattatc
     5881 tctttggagc ggcatgcccc tgtggacaaa caaaaggggt gcgccgcttt ttctgattcg
     5941 gaagcttatg ttccggcgtg cgacttcggt cgctaacgac gccgctcgga gggaatgaag
     6001 atgttatcaa acaacagacc cgtgcgtttc gtggccgtgt gggcgatggc cttgatgatg
     6061 ctggcggcgc cgctcgcggc gctcgcgcag acgcaggtcg tcatgccgaa gaacaagtac
     6121 aacgtgaacg acgacgtgaa gctcgggcaa caggccgcgc agcaggccga gcagcagctt
     6181 ccggtcttgc gcgacgagtc cgtcgaaagc tacgtcgcgc gggtggggca acggctcgtc
     6241 agcgcgatcc cgccgcagtt tcagcagccg cagttccagt accgcttccg cgtcatcgac
     6301 gcgcgcgaca tcaacgcgtt cgcgctgccg ggcgggccga tgtacgtcaa ccgcggcatg
     6361 atcgaagcgg cgcgcaccga gggcgagatg gccggcgtga tggcgcacga aatctcgcac
     6421 gtcgctctgc gccacgcgac ggcgcaggcc accgagacgc agaagtttca gatcggctcg
     6481 gtcttgggtc aaatcgccgg cgcggtgatc ggcggcccgg cgggcgcggt gattggcacg
     6541 gcgagccaga tcggcttcgg cgcgggcgcg ctgaagtaca gccgtaagta tgagacgcag
     6601 gccgatatcc tcggctcgca gatcatggcg cgcgccggct acgacccgcg cgacctcgcc
     6661 aacatgttcc gcaccatcga gggacagggc ggcggcgccc gcggccccga gtggatgtcg
     6721 agccacccga accccggcaa ccgctacgag cgcatcaatc aggaggcggc catgctgcgc
     6781 gtctccaacc cgaaccgcga catgagggag ttcagccaga ttcaggcgcg cctgcgcggc
     6841 cagccgcgcg cccgctccat ggaggagatc gcgcgctcgg gccagaccaa cccgcagggg
     6901 acgcgctacc cggataatcg ttacccggac aaccgtgaca cacgctaccc ctcgggcgag
     6961 cgcgtggcct atccctcgac gcgtctgcgc gcctaccagg ggagcttctt ccgtctcggc
     7021 gtgcccgaca actggcgcga gctcggcggg cagcagaatg ctgtgacgtt cgcgcccgac
     7081 ggcgcctaca gccagcaggg cgagttcacg cacggcatga tggtgggcat cgcccccgcg
     7141 cagtcgcgcg acctgcgcgg cgcctcggac gaatttcttc agggcctgct tcagggcggc
     7201 aacagctacc tgcgcccgca gagccgcggc taccagcgcg cctccatcgg cggccggagc
     7261 gcgctcgcca tgccgctcac ggggcgctcg ccgctgacgg ggcagaacga atacgtcgtc
     7321 gtctacacga cgcagctccg cgacggcaac gtgttctatc tggtcaccgt cgccccgcag
     7381 agcgactaca actcgttcga gcgcgccttc cgaagcgtct taagctcggt tcagctcaac
     7441 gactaaaggc ggtcggcctg gagtccttaa cctcggaagc gaagggttgt ccgccggagg
     7501 cccttcgctt ccgcatttaa gaccggaact ttcgtgagga gacgaactcg tggcaagagg
     7561 ttgggagagt aaatcggtgg ccgaccagat ggacgcggcc ggcgccgcgc cggcgcgtga
     7621 ggattcgcgc tacgaagtgt cgccggccgt gcgcgagcga cagacgcgtc tgcgctggct
     7681 gcggctttca cacgcgcgca cgtccgaaca actcaagcgc gccaccaacg acgcgcaccg
     7741 gcagatgctc gggcgcgcca tccgcgcgct cgaacgcgag atggaagatt tagtttaagg
     7801 aagggcgaag cgtcttcgga aaagggcgcg gcgggcggcg acgttctaac gttagaacgt
     7861 cg
//
LOCUS       DASVES010000082         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1429614, whole genome shotgun sequence.
ACCESSION   DASVES010000082 DASVES010000000
VERSION     DASVES010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429614"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VF240_02935"
     CDS             complement(<1..831)
                     /locus_tag="VF240_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX7174230.1"
                     /translation="MMRTKLLLRRAAFALALLAPQTLALAAETHRFTPKIGYPTFAKR
                     PPVLRIKPGDTVETSTLWGEWYERAGGAWPGEVGPFHVEGAAPGDTLVVRIVRLRPNR
                     DTAVSTHTPGFSALASDKYTPMLNDPVPARRFIWRIDRTRNTGTLDLPASRLKRIEVE
                     LQPMLGRVAVAPPGEQSWGGLWPGEFGGNMDASDVREGATVYLPVFHEGALFYFGDGH
                     ALQGDGEVCGSGLETSMDVVFHFELRKGKRIRWPRIENDEYIMATGSVRPLMDAFRIA
                     Q"
     gene            1134..2462
                     /locus_tag="VF240_02940"
     CDS             1134..2462
                     /locus_tag="VF240_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7174231.1"
                     /translation="MINKTFVIRLVAALLILLFLLAVLAPLSGCRQGGPTGGDSGGVK
                     QLSLAVNSGVEGDALKAAARDYEAQTGVRVQINEFPYNNLFEKELIDLQAATGAYDLI
                     MLDDPWFPRFSTQGYLTDFTPLYEKKNLRGPDSDFVSTSLALCREPYETGTLHALPYV
                     GNSQLFFYRRDLFEKHSLRPPATWDDVLAAARTIHEREASGAGGGRVNGYVMRAAQGN
                     AVVADFMPIFWAFGAEMFDAEGRPTVNSSEGVAALKFMLELGKYAPPGYQSFNADEVS
                     AHLLQGTAAMSINWPAWISAFSDPARTKYVGLVEFGPMPGASKPGRAEIGNWLIAIPR
                     ASRNAEAAFDFLLWATSAEQMKRSALRGNPPTRKSVFTDAELLAKFPAYPAQLRSLET
                     SRPRPRTPLWNEIENAFGVFLSKANSGELTPEDAMNQANARIAEIVARGR"
     gene            2459..>2800
                     /locus_tag="VF240_02945"
     CDS             2459..>2800
                     /locus_tag="VF240_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174232.1"
                     /translation="MTRASRHEAGYERALPYLLVAPAVAVLVALSIYPLVYAVRVSLQ
                     TGAGERARWSLQNFSRLASDAFFLDALAHTLVFATAALTIEFALGLSLAVLLNREMRG
                     RGIFRAALLVPM"
BASE COUNT          434 a         1004 c          884 g          478 t
ORIGIN      
        1 ctgcgcgatg cgaaaggcgt ccatcagggg ccggacgctg cccgtcgcca tgatgtactc
       61 gtcgttctcg attcgcggcc agcggatgcg cttgcccttc cgcaactcga agtggaagac
      121 gacgtccatg ctcgtctcca gccccgagcc gcagacctcg ccgtcgccct gtagggcgtg
      181 gccgtcgccg aagtagaaaa gcgcgccctc atggaagacg ggcagataga cggtcgcgcc
      241 ctcgcgcacg tccgaggcgt ccatgttgcc gccgaactcg cccggccaca gtccgcccca
      301 cgactgctcg cccggcggcg cgacggccac ccggcccagc atcggctgta gctcgacttc
      361 gattcgcttc aggcggctcg cgggcaaatc gagcgtgccc gtgtttcgcg tgcggtcgat
      421 gcgccagatg aagcggcgag ccggcacggg gtcgttgagc atcggcgtgt acttgtcgct
      481 ggcgagcgcg ctaaaacccg gcgtgtgtgt ggagacggcc gtgtcgcggt tgggccgcag
      541 acgcacgatg cggacgacga gcgtgtcgcc cggcgcggcg ccctcgacgt ggaaggggcc
      601 gacctcgccc ggccacgcgc cgcccgcgcg ctcgtaccac tcgccccaca gcgtgctcgt
      661 ctccaccgtg tcacccggtt tgattctcag gacgggcgga cgtttggcga aggtcggata
      721 gccgatcttc ggcgtgaagc ggtgcgtctc ggcggccagc gcgagagtct gcggcgcgag
      781 aagcgcgagc gcgaaggccg cgcgccgtag caagagtttc gttcgcatca ttggtctcct
      841 cttcggtcgg tgtgcggcgc gggcggaagc ccgaccgtga gggagggcat cggggagtcg
      901 gcagtcggca ggaggcagtc ggcagtcagg gcgcttgacc gtcttgactg cttactgctc
      961 actgcttact gctcactcct gacgccctcc ctcacggtcg ggcttccgcc ctgtgcctcg
     1021 cgacgcactt tatgaaatct tcggccgcgc gacaacaaca acaatgacgg ccggggcgaa
     1081 aaggtttact atcttggccg cccgcttaac tctttcagac actttcggcc cgcatgataa
     1141 acaaaacctt cgtcatccgg ctcgtcgccg cgctgctcat cttgctgttt ctcctcgccg
     1201 tcctcgcgcc gctctccggc tgccggcagg gcggccccac gggcggcgac agcggcggcg
     1261 tcaagcaact gagcctcgcg gtcaactccg gcgtcgaggg cgacgcgctc aaggccgcgg
     1321 cgcgcgacta cgaggcgcag accggcgtcc gcgtccagat caatgagttc ccttacaaca
     1381 acctcttcga gaaggaactc atcgaccttc aggccgcgac gggcgcttac gatctcatca
     1441 tgctcgacga cccttggttt ccgcgcttct ccacgcaggg ctatctcacc gacttcacgc
     1501 cgctctatga gaagaaaaac ttgcgcggcc ccgacagtga cttcgtctcg acctcgctcg
     1561 ccctctgccg cgagccttac gaaacgggca ctctccacgc gctcccctac gtcggcaact
     1621 cgcagctctt cttctaccgc cgcgacctct tcgagaagca ctcccttcgt ccgcccgcca
     1681 cctgggacga cgtgctcgcg gcggcgcgca ccatccacga gcgcgaagcg tcgggcgcgg
     1741 gcggcgggcg cgtcaacggc tacgtgatgc gcgccgcgca gggcaacgcc gtcgtcgccg
     1801 acttcatgcc catcttctgg gccttcggcg cggagatgtt cgacgccgaa ggcaggccca
     1861 ccgtaaacag ttccgagggc gtggccgctt tgaaatttat gttggagctt ggcaagtacg
     1921 cgccgcccgg ctaccagagc ttcaacgccg acgaggtctc ggcgcacctg ctgcaaggca
     1981 cggccgcgat gtcgatcaac tggcccgcgt ggattagcgc cttctccgac ccggcgcgga
     2041 cgaagtacgt cggcctcgtc gagttcggcc cgatgccggg cgcgtcgaag ccgggacgcg
     2101 ccgagatcgg caactggctc atcgccatcc cgcgcgcctc gcgcaacgcg gaagccgcct
     2161 tcgacttcct gctgtgggcc accagtgctg agcagatgaa acgctcggcc ctgcgcggca
     2221 acccgccgac gcgcaagtcc gtcttcacgg acgcggaact gctcgcgaag ttccccgcct
     2281 acccggcgca gctccgctcg ctcgaaacct ctcgcccgcg gccccgcacc ccgctctgga
     2341 acgagatcga gaacgccttc ggcgtcttcc tctccaaggc caactccggc gaactcacgc
     2401 ccgaggacgc gatgaatcag gccaacgccc gcatcgccga gatcgtcgcg cgcggaagat
     2461 gacgagggcg tcgagacacg aggccgggta cgaaagggcg ctgccctacc tgctcgtcgc
     2521 gccggcggtg gcggtgctcg tcgcgctctc catctacccg ctcgtctacg ccgtgcgcgt
     2581 cagcctccag accggcgcgg gcgaacgcgc ccgctggtcg ctccagaact tctcgcgtct
     2641 cgcctccgac gcattcttcc tcgacgcgct cgcacacacg ctcgtcttcg cgacggccgc
     2701 gctgaccatt gagtttgcgc tcggcctcag tctcgccgtg cttttgaatc gggagatgcg
     2761 cgggcggggg atatttcgcg cggcgcttct ggtgccgatg
//
LOCUS       DASVES010000083         9072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_335278, whole genome shotgun sequence.
ACCESSION   DASVES010000083 DASVES010000000
VERSION     DASVES010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335278"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /gene="dnaX"
                     /locus_tag="VF240_02950"
     CDS             complement(<1..1629)
                     /gene="dnaX"
                     /locus_tag="VF240_02950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7174233.1"
                     /translation="MTYQVIARKWRPQTFEEVTGQEHITRTLRAGIEHDRLHHAYLFS
                     GARGVGKTTTARLLAKAVNCHRSAGPTPTPCSSKEEEPCPSCREIAEGRSIDVLEIDA
                     ASNTGVDNVRDAIIESVGIRPARDRYKVFIIDEVHMLSPAAFNALLKTLEEPPPRVLF
                     IMATTHAHKVPDTILSRCQQFEFRTISAAKIAERLKLIAKEEGVKIADEALREVARAG
                     EGSMRDAQSAFDQVISFSEGKIGVGDVESALGIAGGEMLARVMRAVAGHAPADALAVV
                     DELVARGHDLRNFCRDLLAHVRDLLVLKAAGEATALADAGEAERRALVEEAADFSESD
                     LVRFFHSLTETETHLRESAHPRYQLEMGLVKLVEMRRLAPLGQILERLSALEEALRTG
                     RAPSAGGNAPATPAPASGTGGGATRRGSGAGGAASSAASSATREDEGGAASRRDASNV
                     RATEVAASARANTPAVSAAPLDLSGAPAAEPTLPATRQPAAAPFATLKLVPPADGSEA
                     VPSAPFADDEPPFPSDGAVRASAKSRAAAAHTTEP"
     gene            complement(1675..2040)
                     /locus_tag="VF240_02955"
     CDS             complement(1675..2040)
                     /locus_tag="VF240_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7174234.1"
                     /translation="MNVRFNRLNHVQLCIPRGAESEARAFYGRLLGLKEIEKPKELKE
                     NGGLWFEMADIQLHVGVEDAVAPSKRHPAFEVDDAPGVRRYLEENGVRTRDEEDVPGM
                     RRFSFYDPFGNRVELMEKI"
     gene            complement(2040..2291)
                     /locus_tag="VF240_02960"
     CDS             complement(2040..2291)
                     /locus_tag="VF240_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174235.1"
                     /translation="MSRDVVDLVLAFETEFGIYVPAADAWKITTPRQVTDYVWDKITH
                     ERMTREQVAAWVREVIVEQTAVERFGDDWHFVRDMGLYC"
     gene            complement(2438..2537)
                     /gene="ffs"
                     /locus_tag="VF240_02965"
     ncRNA           complement(2438..2537)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF240_02965"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2653..2739)
                     /locus_tag="VF240_02970"
     tRNA            complement(2653..2739)
                     /locus_tag="VF240_02970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2703..2705),aa:Ser,seq:gga)
     gene            complement(3066..3500)
                     /locus_tag="VF240_02975"
     CDS             complement(3066..3500)
                     /locus_tag="VF240_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7174236.1"
                     /translation="MHSTRERILFIDEHEDTREVMTALLSRRGCEVVAASSLITALLI
                     SRGQTFHLYVLDAQFWGDTGTELCRSIREFDPLTPIIVFSNRAEEAARREAFEAGATD
                     YLIKPDADGLTKAAGQCIRHCDGRPLFEPPVISASTRLGVVA"
     gene            complement(4356..5060)
                     /locus_tag="VF240_02980"
     CDS             complement(4356..5060)
                     /locus_tag="VF240_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7174237.1"
                     /translation="MPLRKLLFSVTAALLVGLGTATVRADEIYFSEPTSAQGTGFGTV
                     LTVLTLHATPNESGSVSWNGATDVRTGHATNQSQTYSFTQLINSGIENAGSIGLVFNS
                     DETGPVPNSNFLDSLTLNVYNNTTGALVYSVSLRPSDAGDDYPTVEQGIGGSGYLFLL
                     DAGAQASLNAFFLNNPSFRIGLSAAVTGSDNGPDTFYIVDVNTPVPEPTTMLLLGTGL
                     VGIAAKVRRRRRADSE"
     gene            complement(6074..>9072)
                     /locus_tag="VF240_02985"
     CDS             complement(6074..>9072)
                     /locus_tag="VF240_02985"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HEX7174238.1"
                     /translation="SYANNTDAGTAGASATYAGDTNHTGSSDSENFTIDQAPSITTVT
                     CPTNVTYTGAALEPCTATVAGAGSLNQSLSVSYTNNTDAGAANASASYAGDANHTGSS
                     DSENFTIDKASSTTVVTCPTNVTYDGTAQTPCSAVVTGAGGLNQSLMVSYSNNTNAGT
                     ANASATYAGDANHTGSSDSENFTIDPAASTTVVTCPTNVTYTGAAIEPCSAVVTGAGG
                     LNQSLTVSYSNNIDAGTASASASYAGDANHTASSDSKNFTIDQASSTTVVTCPTNVTY
                     TGAAQTPCTATVTGAGGLNQSLTLNYTNNTDAGTATASASFAGDANHTGSSDSKNFNI
                     DQAPSITTVTCPTSVTYTGAALEPCTATVTGAGGLNQSLSVSYSDNVNAGTANASASY
                     GGDANHTGSSDSESFTIDPAASTTAVTCPTNVTYTGAAQEPCTAIVTGAGGLNQALPV
                     SYSNNTDAGTATASASYAGDANHTGSNDSKNFTIDKAATTTVVTCPTNVTYTGAALEP
                     CSATVTGANLSEPVAVNYTNNTNAGAATASATYAESANYLGSSDSKNFTIDKAATTTT
                     DTCPANVTYTGAAIEPCSAAVTGPGGLNETLTVSYADNVNAGTATASATYAETANYLG
                     SSDSENFTIDKAASTTVVTCPASATYTGSALEPCTAAVTGAGGLNQSLTVSYTNNTNA
                     GTATASASYAESANHLGSSDSKNFTIDKAATTTTVTCPTSVTYTGAAIEPCTASATGA
                     GGLNQSLTVSYTNNVNVGAATASATYAASANHLGSTDSETFQITSKALAITANNPSKV
                     YGQTVTFAGTEFTSVGLVGGDSVNSVTLTSAGAATLATVGNYPIVASAAVGTGLSNYT
                     ITYHDGTLTVTTAYCFIGFHSPIGGSVETGNGGSFADPVRAFKLGSTIPVKFTIKEVG
                     CQGSAVTTGIHTLQALKYTNTTDADAPIDATPTDGATTGNQFRLTGTDWHFNLNTKGG
                     FSQGTWLLRATLQDGSVHTVWVSIKK"
BASE COUNT         1516 a         2741 c         3022 g         1793 t
ORIGIN      
        1 cggctcggtg gtgtgcgcgg cggcggcgcg cgacttcgcg gaggcgcgca ccgcgccgtc
       61 cgaggggaac ggcggctcgt cgtcggcgaa aggggcggaa ggaacagcct cggagccgtc
      121 ggcgggcggg acgagcttca atgtcgcgaa aggcgcagcc gcaggctggc gtgtcgcggg
      181 cagcgtgggt tcggccgcgg gtgcgcccga gaggtcgagc ggcgcagcgg agacggcggg
      241 cgtgttcgcg cgggcggacg ccgcaacttc tgtcgcgcgc acgttgctcg cgtcgcgacg
      301 cgaagccgcg ccgccttcat cctctcgcgt cgccgaagat gcggcggacg aggcggcgcc
      361 gccggccccg ctgccccggc gcgtcgcgcc accgcccgtg ccggaggcgg gcgcgggtgt
      421 cgccggtgcg ttgccgccgg cggatggggc gcgtcccgtg cggagcgcct cttcgagcgc
      481 gctcaggcgt tcgaggattt gcccgagcgg cgcgaggcgg cgcatctcca cgagttttac
      541 cagacccatc tccaactggt agcgcgggtg cgcgctctcg cgcaggtgcg tctccgtctc
      601 ggtgagactg tggaagaagc gcacgaggtc tgactcggaa aagtcggcgg cctcttcgac
      661 gagcgcgcgt cgctcggcct cgcccgcgtc ggcgagcgcc gtcgcctcgc cggccgcctt
      721 caggacgagc aggtcgcgga cgtgcgcgag caggtcgcgg cagaagttgc gcaggtcgtg
      781 tccgcgcgcg acgagttcat caacgacggc gagcgcgtcg gccggcgcat gccccgccac
      841 ggcgcgcatc acgcgcgcga gcatctcgcc gcccgcgatg ccgagggcgc tctccacgtc
      901 gccgacgccg atcttgccct cgctgaaact gatgacctga tcgaaggccg actgcgcgtc
      961 gcgcatcgag ccttcgccgg cgcgcgcgac ctcgcgcaac gcctcgtcgg cgatcttgac
     1021 gccttcctcc ttcgcgatga gcttcaggcg ctcggcgatc ttcgccgccg agatggtgcg
     1081 aaactcgaac tgctggcagc gcgacagtat cgtgtcggga accttgtggg cgtgcgtcgt
     1141 cgccatgatg aagaggacgc gcggcggcgg ctcttccaga gtcttcagga gcgcgttgaa
     1201 ggccgcgggg gagagcatgt ggacttcgtc gatgatgaag accttgtagc ggtcgcgcgc
     1261 ggggcggatg ccgacgcttt cgatgatcgc gtcgcgcacg ttatcgacgc ccgtgttcga
     1321 cgccgcgtcg atctccagca cgtcaatcga gcgcccctcg gctatctcgc ggcaggacgg
     1381 gcaaggctcc tcctctttgg acgagcaggg tgtgggcgtc ggccccgctg acctgtggca
     1441 gttgacggcc ttcgcgagca gccgtgccgt ggtcgtcttg cccacgccgc gcgcgccgga
     1501 gaagagatag gcgtggtgca ggcggtcgtg ctcgatgccg gcgcgcagcg tgcgcgtgat
     1561 gtgctcctgg cccgtcacct cctcgaaggt ctgcgggcgc catttgcggg cgattacctg
     1621 ataagtcatg ggaagcggtc agcggtcggc tcttaagtgg tcggcccttc ggcctcaaat
     1681 cttttccatc aactcgacgc ggttgccgaa ggggtcgtag aaggagaagc ggcgcatgcc
     1741 cggcacgtcc tcttcgtccc tcgtccgcac gccgttctcc tccagatagc ggcgcacgcc
     1801 gggcgcgtcg tccacctcga aggccgggtg ccgcttcgac ggcgcaacgg cgtcctcgac
     1861 accgacgtgc aactggatgt cggccatctc gaaccacagg ccgccgttct ccttcaactc
     1921 cttcggcttc tcgatctcct tgagtccgag gaggcggccg tagaaggcgc gcgcctctga
     1981 ttcggcgccg cgcgggatgc agagctggac gtggttgagg cggttgaagc ggacgttcat
     2041 cagcagtaaa gccccatgtc gcggacgaag tgccagtcgt cgccgaagcg ttcgacggcc
     2101 gtctgttcga cgatcacctc gcgcacccag gcggcgacct gctctctcgt cattcgttcg
     2161 tgtgtgatct tgtcccagac gtagtcggtc acctgacgcg gcgtggtgat cttccaggcg
     2221 tcggcggccg ggacgtaaat cccgaactcc gtctcgaagg ccaacaccag atcgaccacg
     2281 tctcggctca tgtgggaaac tttgtcggac aaagaaaaag agttgccgcg cgccggcgac
     2341 gcgctcgtct cgaagcttcg atgctggatt gtgctccaac ctcgaagctt tcggcaagcg
     2401 cgacgccgcg cggggcaact ctttccccgt tttggccaga ctccggacta acccgcaaca
     2461 cacgccgccg ccgctaccgt tgctccgttc ccggcctggc ggggttcgcg gggcaaggcg
     2521 ttgtgcggag cccgaagtct gataagtttt caaaagctat tagctgttag ctgttggcga
     2581 ttagctttca gccgtcagct taacataaag aaagctaaat gctaatcgct aacagctagc
     2641 ggcttttcgg aacggagagg gtgggattcg aacccacggt acggttcccc gtacacagca
     2701 tttccagtgc tgccagttca accactcctg cacctctcct gaagcagaca ataggtaaaa
     2761 ataaatggca aaattaggta ggatttcaaa ctacccgcag ccggcaaagc atagcctatc
     2821 tcggcgattc atgtcacgtc cgtcacgccg acaacgcgcc gggttgggat aaggaatggt
     2881 ctggaagaag gttcagctcg tgatgtcagg gaggccagct cttcttgcgc cagttggcgc
     2941 gaggcgcgtg aagggcgccg gaagcgcgac cgtctcaacg agtgagttgg aaagagttgt
     3001 ggcggagagg gtgggagtca cttccccacg gcggcctttc ggccatcgcg gttttcgagt
     3061 ccgccttacg cgacgacgcc gaggcgtgta gacgcagaaa ttaccggagg ctcaaataag
     3121 ggccgtccgt cacaatgcct gatgcattgg cccgccgcct tggtcaggcc gtctgcgtcc
     3181 ggcttgatca agtaatcggt ggccccggcc tcaaacgcct cccgccgcgc cgcttcctcc
     3241 gccctgtttg agaaaacaat gatcggggtg agggggtcga actcgcggat gctccggcac
     3301 agctcggttc cggtgtcgcc ccagaactgc gcgtcgagca cgtagaggtg aaaggtctgc
     3361 ccgcgcgaaa tgagcagcgc cgtgatgaga ctggaggcgg cgaccacctc gcagcccctg
     3421 cgcgacagca acgccgtcat gacttcgcgg gtgtcctcat gctcgtcgat gaagagtatg
     3481 cgctctctgg ttgaatgcat tacggttgcc tctgagtatg cctgagaagg attcggcggg
     3541 gtgtcattcc atcgccggct tcggggctgg cgacggactc atgggcgctg atagctttta
     3601 gcgcacgagg agtcgcccct tgagcggcca ctcatctatg ttcagcgacc gtgcgggagt
     3661 gtaaacaaat gggcacccga gcacaacgca caaatctccc aattttcccc ggtataattg
     3721 tcatggggta acgcggagag tttcggtgcg ggccgggggc ggcgggttga ggttagcagt
     3781 gcgccccgct gagtttgtcg gacagcgttg aggcggtgtc gtcagtggtt aggtagaacg
     3841 aggggccttt tcgtcgtttc tactgggtta caacgatccg aaggaaggcg agagcctccc
     3901 gaccgttggg cagtggatag tctcccccat gcctaccctc tgcgcggcgg gcctcgccgc
     3961 cctcatctgg tgcccgtgac ggcgtaatag aagaagggcg gcggggattg ttcgcccaac
     4021 cccgccgccc ttgacctccg gctgcctcga tttcagccgc ttaatgagta cgcgggccgt
     4081 cgcaccctcg gatcgacacg cgctcgctcc atccgcggtg ggttcctcat gagtgaggat
     4141 gaggtgatca gataagacag cgtttcacac acctccttcg accccgaggc gatctgacgc
     4201 ccgctttcgt cgaacaggcc gaaaacgctc ccgccggagt ccaagggcaa gactactggc
     4261 ttcgcctcca taggactctc ctctccaaat cttacactca aacgtatggg cgggtgacta
     4321 ccgggtcaac gttttgccgt cccggcaggc gccggtcact cggaatctgc ccggcgcctg
     4381 cggcgcacct tggcggcgat gcccaccagg cccgtgccga gcaggagcat cgtcgtcggc
     4441 tccgggaccg gagtgttgac gtcaacaatg tagaaggtgt cggggccgtt gtccgaacct
     4501 gtaacggcgg ccgacaaccc aatgcggaag ctcgggttgt tgaggaaaaa cgcattgagc
     4561 gaggcctggg ctcccgcgtc caggaggaac aggtatccgg acccgccgat gccctgctcg
     4621 accgtggggt agtcatcgcc ggcgtcggaa ggacgcaagg acacgctata caccaaagcg
     4681 ccggtagtat tgttgtagac gttcaacgtc agcgagtcga ggaaattgct gttggggaca
     4741 ggccccgtct catcggagtt aaagacgagg ccgatgctgc cagcgttttc gatgccgctg
     4801 ttgatcagtt gcgtgaacga gtaggtttgc gactgattag tcgcgtggcc cgtcctcaca
     4861 tccgtggcgc cgttccatga gactgagccg gactcgttcg gggttgcgtg cagtgtcaga
     4921 accgttagca cggtgccaaa gccggtccct tgggccgagg tcggctccga gaaatagatt
     4981 tcgtcggcgc gcaccgtggc ggtgccgagg ccgacgagca atgccgccgt tacgctaaag
     5041 agtagtttcc tgaggggcat gagagactcc tttgaatttt ccattttatc tttgtcaagg
     5101 cacgtcagaa agggcagtca ccgcactgac tgcttggtgg cggagcgggg caagcgcacc
     5161 ggacgccggt catccggcat tttggggaga gacatcagac gccacgtcag cacgggcaaa
     5221 gcgcatcagc gcacgtctta tgtgtagact cttgtccagc atcttacgcg aaagggtggc
     5281 agcgcgcatg ggacaaagcg acaatttttc gtagtacaaa tgtcctgggg atttacttag
     5341 cgtcgtaggc ggacgcggtg aaagggccag aagtggagtc gggcaggcgc gggtcttaga
     5401 ataccgacct cagcgttgac tgaggcgctc tgactcgatg cccattcggg agtggagttc
     5461 gatgtgtagg tgcccgccgc ccgagtgggt taacgcctga tcttcaaaag cgcgggcgag
     5521 gtggccttaa ggccgtgaag tgagacgccc ggcggatgtc gtccgggggc gattgcgtgc
     5581 aaaaaaaggg cgccggttac ctcggcgccc gagaccacag gaggaaactg tgttgtcggt
     5641 tggatgcggg aacgcctcct caggtgcgcc gcctcgcaag cccgtccaaa tgaaaaattt
     5701 tgaaaggaaa tgaaagctat tttcggcgaa ttttcttaac caatataaac ctagtcgcag
     5761 atgcgcttgg ctttgatgca aaaatccaac agcaccccaa catactgagt cgaatgggat
     5821 gctctggctc aactatagcg gtttgcagtt gggtgtaacc gaaccatcga cggtttagag
     5881 gcgggctatg ccagccgtca caaccctcga atcttgagcc ggcgggcatc gcccgcctct
     5941 aaaccgtcgc actctactgc aaaccgctat agttaaatat agaaggcggg caggacccag
     6001 agtcccgccc gccttccggt tgtaagggag cgtcctgtgc tgccactgac gcctgcccct
     6061 ggggaagtag ttgttacttc ttgatcgaga cccacaccgt gtgaacgctg ccgtcctgaa
     6121 gggtggctct cagcagccag gtgccctggc tgaatccgcc cttggtgttc aggttgaagt
     6181 gccaatcggt gccggtcagc cggaactggt ttccggtggt ggccccgtca gtgggcgtgg
     6241 catcaatcgg tgcgtccgcg tcggtagtgt tggtgtactt gagcgcctgc agggtgtgga
     6301 ttccggtggt caccgcgctg ccctgacaac cgacttcctt gattgtgaac ttgaccggaa
     6361 tcgtgctgcc cagtttgaaa gccctgacgg gatccgcgaa gctgcccccg ttcccggttt
     6421 cgactgagcc gcctatgggc gagtggaacc cgatgaagca gtaggccgtc gtgaccgtca
     6481 gcgtgccgtc gtggtaggtg atcgtatagt tcgacagccc agtgccgacg gccgcgctcg
     6541 ccacaatcgg ataattaccg accgtggcga gagtggccgc gcccgcgctg gtcagcgtca
     6601 cgctgttcac cgagtcgcca ccgaccaggc cgacggaagt aaattccgtc ccggcgaacg
     6661 taaccgtctg gccatagacc ttcgacgggt tgttggccgt aatcgcgagc gccttcgacg
     6721 tgatttggaa ggtctccgag tcggtgctgc cgagatggtt agcgctcgcc gcataggttg
     6781 cgctggccgt cgcggcgccg acgttgacgt tgttcgtgta gctgacggtc aaggactggt
     6841 ttagcccgcc cgccccggtg gccgacgccg tgcaaggctc gatggccgcg cccgtgtagg
     6901 tgacgctcgt cggacaggtc acggtcgtcg tcgtcgccgc cttgtcgatg gtgaagttct
     6961 tcgagtcaga gctgccgagg tggttggcac tctccgcgta gctggcgctc gcggtggccg
     7021 tcccggcgtt ggtgttgttc gtgtaggaca ccgtcagcga ctggttgaga ccaccggcgc
     7081 ccgtcaccgc cgccgtgcac ggctcgaggg ccgaaccggt gtaggtcgcg ctcgccgggc
     7141 acgtgacgac ggtcgtcgaa gcggccttgt cgatggtgaa gttctcactg tcgctgctgc
     7201 cgaggtagtt ggcggtctcg gcgtaggtgg cgctggccgt ggccgtgccc gcgttgacgt
     7261 tgtcggcgta gctgaccgtc agcgtctcgt tcagtccacc cggccccgtc accgccgccg
     7321 agcaaggctc gatggccgca cccgtatagg taacattcgc cgggcaggtg tcggtcgtgg
     7381 tcgttgcggc cttgtcgatg gtgaagttct tcgagtcgga actgccgagg tagttggccg
     7441 actcggcata cgtcgcactg gccgtagcgg cgccggcgtt cgtgttgttc gtgtagttga
     7501 ccgcgaccgg ctcgctcaga ttcgcaccgg tcaccgtagc ggagcacggc tcgagtgccg
     7561 cccccgtgta agtgacattc gtcgggcagg tcaccacagt ggtggtggcc gccttgtcga
     7621 tggtgaagtt cttcgagtcg ttgctgccag tgtggttggc gtcaccggcg taagacgcac
     7681 tggccgtggc cgtcccggcg tcggtgttgt tcgagtaact tactggaagc gcttgattca
     7741 aaccgcccgc gcccgtcacg atggccgtgc agggctcctg ggccgcacct gtgtaggtca
     7801 cgttcgtcgg gcaggtcacg gcggtcgttg acgcggccgg atcgatggtg aagctctcag
     7861 agtcgctgct ccccgtgtgg ttcgcgtctc ccccgtagga tgcgctcgca tttgcggtgc
     7921 ccgcgttgac gttatctgaa taactcaccg aaagcgactg gtttaggccc cccgccccag
     7981 tgaccgtggc ggtacacggc tcaagcgccg cccccgtgta ggtgacgctc gtcgggcagg
     8041 tcaccgtcgt gatggaaggc gcctgatcga tattgaagtt cttgctgtca ctgctgccgg
     8101 tgtggttggc atcgccggcg aatgaagcgc tcgccgtcgc cgtgcccgcg tccgtgttgt
     8161 tcgtgtagtt gagcgtcaac gactgattga ggccgcccgc gcccgtgacc gtggccgtgc
     8221 agggcgtctg cgccgcgccc gtgtaggtga cgttagtcgg gcacgtcaca acggtcgtag
     8281 acgaagcttg gtcgatagtg aaattcttcg agtcgctgct tgccgtgtgg ttggcgtcgc
     8341 cggcataaga cgcgctggcc gaagccgtcc cggcgtcgat gttgttcgag tagctcaccg
     8401 tcaacgactg attcagcccg ccagcgcctg tgactaccgc cgagcagggt tcgatggccg
     8461 cgcccgtgta agtgacgttg gtcgggcacg tgacgacggt cgtcgaagcg gccggatcaa
     8521 tcgtgaagtt ctcactatca ctgctgccgg tgtggttcgc atcaccggcg taggtcgcac
     8581 tcgcgttcgc ggtgcccgcg ttcgtgttgt tcgagtagct taccatcagc gactggttga
     8641 gaccgcccgc gcccgtgacc accgccgagc agggcgtctg cgccgtgccg tcgtaagtga
     8701 cgttggtcgg gcaggtgacg acggtcgtgg acgaagcctt gtcaatggtg aagttctccg
     8761 agtcgctgct ccccgtatga ttcgcgtcgc cggcgtatga agcactcgca ttagcggcgc
     8821 ccgcgtcggt gttgttcgtg tagctcacgg aaagcgactg gtttaggctc ccagcaccgg
     8881 cgactgtcgc cgtacacggc tcaagcgccg cccccgtgta ggtgacgttg gtcgggcagg
     8941 tcaccgtcgt gatggaaggc gcctgatcga tggtgaagtt ctcactgtcg ctgctgccgg
     9001 tgtggttcgt atcaccggcg tacgtggcac tcgctccggc cgtgccggcg tcggtgttgt
     9061 tcgcgtaact ga
//
LOCUS       DASVES010000084         8554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1018089, whole genome shotgun sequence.
ACCESSION   DASVES010000084 DASVES010000000
VERSION     DASVES010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018089"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1029)
                     /locus_tag="VF240_02990"
     CDS             complement(76..1029)
                     /locus_tag="VF240_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEX7174239.1"
                     /translation="MIQTGMGGHFYYCSELSLAAGEETFGTASGGEVWMLVEYPLAWG
                     TKALEMSALPRVVKTHLSGLLKIIPRSRVLLIKQQSRSRKSSFSFFVVRTTEGSPWVA
                     RFELTQYEQLLRFDAATVVAAAAVPGADLSTGPLFLVCTHGRRDKCCAKFGYPLFKSL
                     RRHAGASVWQSSHVGGDRFAANLVCFPHGLFYARVTEEAGRRILGEYEGGRVVLENYR
                     GRACYANAVQAAEFFVRRESGLAGVNDLRLRGMTREGERTRRVRFETADGAALHEALI
                     TWGDSSFRNVITCHAGEERSVTQYALGDYRVERAASPLAKA"
     gene            complement(1026..2228)
                     /locus_tag="VF240_02995"
     CDS             complement(1026..2228)
                     /locus_tag="VF240_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7174240.1"
                     /translation="MSFDSLEKLLEPASTAEFLDSAWGRTFKHVRGGAGKFAPLLPWP
                     ALNDILRRHRLDYPRLRLMKDGARVPTNTYLRHAGGASGRPAAVPRLLPAGFTTALRD
                     GATLVLDAADELHAPLEELAETLELRFHERVQINVYAGWRTSRGFDLHWDDHDVFILQ
                     VSGRKRWRLHGETRVCPVAGERDVTPEPAGEPVWEETLEDGDLLYIPRGWWHVALPLD
                     EPTLHLTVGVHKRTGVDLARWLAERLSQHEVFRRDLPRLASADEKRAHAEALKAALLE
                     EWADDAAAIYLAELDATALPRPRLSLPWAATRKLLPAGPGVVVRWTPPRPVEFKVEGG
                     VVEFRCLKRRWRFAESALAVLGPLAERRVCALAELCEGAVGRVEEQRVRALVGELVEH
                     GLAAVVEA"
     gene            complement(2301..2612)
                     /locus_tag="VF240_03000"
     CDS             complement(2301..2612)
                     /locus_tag="VF240_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174241.1"
                     /translation="MADQINPGRGEEQESQESGLFLSSEPIEGGGSPLRAMGEDTDAG
                     DTDAGDADAGDDNGPEEKGDLADGGADTDNSDESADAMDIIGDNMDGTTTKDADATDA
                     K"
     gene            2781..4796
                     /gene="treZ"
                     /locus_tag="VF240_03005"
     CDS             2781..4796
                     /gene="treZ"
                     /locus_tag="VF240_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX7174242.1"
                     /translation="MSATKVRRRLPVGAEVAPDEGGVHFRVWAPRRTKVEVVVESGDG
                     VEGVERGVVVAAIELQPEGGGYFSAPAGEARDGSLYRFRLDGDDSYLYPDPASRFQPR
                     GPHGPSRVVDPAQFKWTDDAWQGHELRAQVIYELHVGTFTREGTWAAAARELAELAAL
                     GVTLIEVMPVADFPGRFGWGYDGVNLFAPTRLYGEPDDMRRFVDEAHRTGLAVILDVV
                     YNHLGPDGNYLRQFSEDYFTDRYVTDWGEAINFDGPGSEGVRELFVANAGYWIDEFHL
                     DGLRLDATQNIYDASGEHVLAAVTRRVREAAGERASIVVAENEPQHTRLVRAPERGGY
                     GMDGLWNDDFHHSAMVALAGRAEAYYADYRGTPQEFISALKYGYLFQGQYYKWQRQRR
                     GTPAFGLPPAVFINFIQNHDQVSNSARGERAHRLSSPGRYRAMTALLLLAPSTPMLFQ
                     GQEFASSAPFYFFADHNEELARLVRKGRVEFLAQFRSLATKETRAHLPPPEDLAIFER
                     CKLDFSERVRHAHVYEMHRDLLRLRREDAVFSAQLQRGIDGAVLGAEAFVLRFFDPHG
                     DDGGGDDRLLLVNLGPDLNLNPAPEPLLAPPENTVWRTLWSSEEPRYGGGGTPPLETR
                     YNWILPGHSAVAMRPAPAGETYDPARGGDLMSEEEETRTEAMKGWDK"
     gene            4816..6864
                     /locus_tag="VF240_03010"
     CDS             4816..6864
                     /locus_tag="VF240_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX7174243.1"
                     /translation="MIEPVRRIVFTEDETHDDERLLSREWLVTNGLGGFASGSISGVP
                     TRRYHGLLIAALSAPLGRIVMLNHLSEHVRFSDGRRVLFGGQERSGGKLQLPGIHYLK
                     EFRLEAGLPFWRFEVDGYRIEKRVLLPHMQNTVHVSYKLLAGDGPVRLKLRPAVQFRP
                     LEAPVGQPHTKSYHFSADDHLMELSGGSDEYPPLRLRLYGERATLTLHSKKLSNVFYR
                     VEQRRGYDHTAELYSPGYFGVSLDVGQEATLVASTESWQTVNALAPEEALPAEFERRR
                     RLVAAAPTPAREGFAAELVLAADQFVITPAGRREDAARARASGEEARSVIAGYHWFTD
                     WGRDTMISFEGLTLTTGRHREAEWILHTFAHYVRDGLIPNMFPDGSNEGLYHTADATL
                     WYFHAVDRYLQTTGDRVTLDILLPKLLDIVAHHLKGTRFRIGVDAADGLLRQGEEGYQ
                     LTWMDAKCDGWVVTPRRGKAVEINALWYNALRLTEGWVRETQGEAAARPFREHAERAY
                     ESFNRRFWYEEGAHLYDVVDTEGGTDDPACRPNQLFALSLRHPVLDPARWESVLTVVR
                     DKLLTPVGLRSLSPDHPEYHSKYDGDLRARDAAYHQGTVWGWLIGPYVDAWLRVRPND
                     RAGARALLEGFRAHMDEACIGSISEVFDAEPPYTARGCVAQAWSVAEVLRCWVMTSEK
                     "
     gene            6992..8527
                     /gene="malQ"
                     /locus_tag="VF240_03015"
     CDS             6992..8527
                     /gene="malQ"
                     /locus_tag="VF240_03015"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7174244.1"
                     /translation="MRFKRSSGVLLHPTSLPGRFGVGDLGAAALKFVDWLADAGQTFW
                     QIMPLGPTGYGDSPYSSLSAFAGNTLLIRPEHLVERGLLGADDLRDAPASSAARVDYG
                     RVIDHKRTLLEKAYKKFKSGSDAGARGDYDGFLQYAASWLDDYALFAALRDKHGGESW
                     HTWEAGVARREEGALARAREELREQVEAHKFFQYLFFEQWLRLKAHARERGVWVVGDM
                     PIFVAHNSADVWARPHLFKLEEDGRPSAVAGVPPDAFSKTGQLWGNPVYDWERMSSEG
                     FVWWTERVRETLKLVDVLRLDHFRGFAAYWEVPAGHETAERGRWVDAPGRELFRALKD
                     ANGGNDLPIIAEDLGTITPDVHALRDEFDFPGMRVLQFAFGGDPRDTHLPHNYTHNTV
                     VYTGTHDNDTTLGWLRARSEDAADGDEGLRRERANCLKYLATDGAEINWDFIRAAHAS
                     VADISIVPLQDVLGLDSDARMNIPASTEGNWAWRFREGALTDALRDRLREMTETYGRL
                     PSQ"
BASE COUNT         1387 a         3094 c         2682 g         1391 t
ORIGIN      
        1 gctccgtccg caccttcggt cgcacgtgtc tccattttct tccctcgctt gtgtgatagg
       61 tggcgtgtgc cccgctcacg ccttcgcgag cggactcgcc gcacgctcga cgcggtagtc
      121 gccgagcgcg tactgtgtga cgctgcgctc ctcgcccgca tggcaggtga tgacgttgcg
      181 gaagctcgaa tcgccccacg tgatgagcgc ctcgtgcagc gcggccccat cggcggtctc
      241 gaacctcacg cgccgcgtcc gctcgccctc gcgcgtcatc ccgcgcagac gcaggtcgtt
      301 cacgcccgcc agccccgact cccgccggac gaagaactcc gcggcctgaa ccgcgttggc
      361 gtagcaggcc cgcccgcggt agttctccaa aaccacgcgc cccccctcgt actcgcccaa
      421 aattctccgg cccgcttcct ccgtcacacg cgcgtagaac aggccgtgcg ggaagcagac
      481 gaggttggcg gcgaaacggt cgccgccgac gtgcgacgac tgccacacgc tcgcgcccgc
      541 gtgccggcgc agagatttga agagcggata gccgaacttc gcgcagcact tgtcgcgccg
      601 cccgtgcgtg caaaccagaa agagcggccc cgtggacagg tctgcgccgg gcaccgccgc
      661 ggcggcgacg accgttgcgg cgtcgaagcg cagcaattgc tcatactgcg tcagctcgaa
      721 gcgagcgacc cacggcgacc cctcggtcgt gcggacgaca aaaaaactga aggagctttt
      781 gcgcgagcgc gactgctgct tgatgagaag cacgcgcgag cgcgggatga ttttcagaag
      841 ccccgacagg tgcgtcttga ccacacgggg cagcgcgctc atctccagcg ccttcgtccc
      901 ccacgcgagc gggtactcga cgagcatcca cacctcgccc cccgaggccg tgccgaacgt
      961 ctcctcgccg gccgccagcg acagctcgga gcagtagtaa aagtgtccgc ccattcccgt
     1021 ctgaatcatg cttcgaccac cgccgccagc ccgtgctcga caagctcgcc gacgagcgcg
     1081 cgcacccttt gttcttcaac ccttccgacc gcgccctcgc acaactccgc gagcgcgcac
     1141 acgcgtcttt cggcgagcgg gccgagcacc gccagcgcgc tttcagcgaa acgccagcgc
     1201 cgcttcaaac agcgaaactc gacgaccccg ccttcgacct tgaactccac ggggcgcggc
     1261 ggcgtccagc gcacgaccac gcccggcccc gccggcagca gcttgcgcgt cgccgcccac
     1321 ggcaaactga gacgcggacg cggcaaggct gtcgcgtcca gctccgcgag gtagatcgcc
     1381 gccgcgtcgt cggcccactc ttcgagaagc gccgccttca gggcttcggc gtgcgcccgt
     1441 ttctcatctg ccgaggccag acgcggcagg tcgcgcctga acacttcgtg ctggctcaga
     1501 cgctcggcga gccagcgcgc gaggtcaacg cctgtgcgtt tatgcacgcc cacggtgagg
     1561 tggagcgtcg gctcgtctaa cggaagcgcg acgtgccacc agccgcgcgg gatgtagagc
     1621 agatcgccgt cttcgagcgt ctcctcccac acgggttctc cagccggctc cggcgtcacg
     1681 tcgcgctcgc cggcgacggg acacacccgc gtctcgccgt ggagtcgcca acgcttgcgg
     1741 cccgacactt gaaggatgaa aacgtcgtgg tcgtcccagt gaagatcgaa gccgcgcgag
     1801 gtgcgccagc cggcgtacac gttgatctgc acgcgctcgt gaaaacgtag ctcaagggtc
     1861 tcggccagct cttcgagcgg cgcgtggagt tcgtcggcgg cgtcgaggac taaggtcgcg
     1921 ccgtcgcgca gggccgtggt gaagcctgcg gggaggagac gcggcaccgc cgcggggcgg
     1981 cccgacgcgc ctccggcgtg gcgcagataa gtgttcgtgg ggacgcgcgc gccgtctttc
     2041 atcagacgca ggcgcgggta gtcgagccgg tgccggcgca ggatgtcgtt cagggcaggc
     2101 cacggcagca gcggggcgaa cttccccgcc ccgcccctga cgtgtttgaa cgtccgaccc
     2161 cacgcggagt cgaggaactc cgctgtcgat gccggctcca gaagtttttc gaggctgtcg
     2221 aaactcacgc ctctgctgtg gaagctcacg cctctgctat cgaagctcac gcctctagcg
     2281 gccacgcacg ccgacaccgt ctacttggcg tcggtggcgt cggcgtcctt cgtggtcgtg
     2341 ccgtccatgt tgtcgccgat gatgtccatg gcgtcggcgc tctcgtcgga gttgtcggtg
     2401 tcggcgccgc cgtcggcgag gtcgcccttc tcctcggggc cgttgtcgtc gccggcgtca
     2461 gcatcaccgg cgtcggtgtc gcccgcgtcg gtgtcctcgc ccatcgcccg caggggcgag
     2521 ccgccgccct cgattggctc gctcgaaagg aaaagccccg actcctgtga ctcctgctcc
     2581 tcgccccggc cggggttgat ctggtctgcc atcttctgct gcctcctgat tgtgttgttg
     2641 gaaaagtggc ctaagcttat tacgagcggg atgaaacgac aagtggcact ccccgctgca
     2701 ttcgtgtgac aacgacgccg cgcgcggtta gaatccgacg cgagcctaat tcaaatctaa
     2761 ccggagcatc attgccagca atgtcggcga cgaaagttcg caggagactg cccgtcgggg
     2821 ccgaggtcgc gcccgacgag ggcggcgtcc acttccgcgt gtgggcgccg cgacgcacga
     2881 aggtcgaggt cgtcgtcgaa tcgggcgacg gcgtcgaggg ggtggagcgc ggcgtcgtcg
     2941 tggcggccat cgagttgcag cccgaaggcg gcggctattt ttccgcaccc gcgggcgagg
     3001 cccgagacgg ctcgctctac cgcttccggc tcgacggcga cgactcttac ctctaccccg
     3061 accccgcctc gcgcttccag ccgcgcgggc cgcatggccc ctcgcgcgtc gtcgatcccg
     3121 cacagttcaa atggactgat gacgcttggc aaggccacga actccgcgct caggtcatct
     3181 acgagctgca cgtcggcacc tttacgcgcg agggcacgtg ggccgcggcc gcgcgcgaac
     3241 tcgcggagct ggccgcgctc ggcgtcacgc tcatcgaggt gatgccggtc gccgactttc
     3301 ccggtcgctt cggctggggc tatgacggcg tcaacctgtt cgcccccacg cgtctctacg
     3361 gcgagccgga cgacatgcgc cgcttcgtcg atgaagcgca ccgcacgggc ctcgccgtca
     3421 tcctcgacgt ggtctacaac cacctcggcc ctgacggcaa ctacctccgg caattctccg
     3481 aagactactt caccgaccgt tacgtgaccg actggggcga ggccatcaac ttcgacggcc
     3541 ccggctccga aggcgtccgc gaactcttcg tcgccaacgc cggctactgg atcgacgagt
     3601 tccatctcga cggcctgcgc ctcgacgcga cgcagaacat ctacgacgct tcgggcgagc
     3661 acgtcctcgc ggccgtcacg cggcgcgtgc gcgaggcggc gggcgagcgc gcgtcgattg
     3721 tcgtcgccga gaatgagccg cagcacacgc gcctcgtgcg cgcgcccgag cgcggcggct
     3781 acggcatgga cggcctctgg aacgacgact ttcatcacag cgcgatggtc gcgctcgcgg
     3841 gccgcgccga ggcttattac gcggactacc gcggcacgcc gcaggagttc atttcggcct
     3901 tgaagtacgg ctacttgttc cagggccaat attacaagtg gcagcggcag cggcggggaa
     3961 cgcccgcttt cggccttccg cccgccgtct tcatcaactt catacagaac cacgatcagg
     4021 tctcgaactc ggcgcgtggc gagcgcgctc atcggctctc cagtcccggc cgttaccgcg
     4081 cgatgacggc gctgctgctc ctcgccccct cgacgccgat gctctttcag ggacaggagt
     4141 ttgcctcctc cgcacccttc tatttcttcg ccgaccacaa cgaggagctg gcccgcctcg
     4201 ttcgcaaggg ccgtgtcgag ttcctcgcac agttccgcag cctcgcgacg aaagagacgc
     4261 gcgcgcacct tccaccgccc gaagacctcg cgatcttcga gcgttgcaag ctcgacttct
     4321 ccgagcgcgt gcgccacgcg cacgtctacg agatgcaccg cgacctgctg cgcctgcggc
     4381 gcgaggacgc cgtcttctcc gcgcaactcc agcgcggcat cgacggcgcg gtgctcggcg
     4441 cggaggcgtt cgtcctgcgc ttcttcgacc cgcacggcga cgatggcggc ggcgatgacc
     4501 gcctgctcct cgtcaacctc ggccccgacc tcaacctcaa tcccgcgccc gagccgttgc
     4561 tcgcccctcc cgagaacacc gtctggagga cgctctggtc gagcgaggag ccgcgctacg
     4621 gcggtggggg cacgccgcct ctggagacgc gctacaactg gattctgccc ggccactcgg
     4681 ccgtcgccat gcgcccggcc ccggccggcg agacctacga cccggcgcgc ggcggcgacc
     4741 tgatgagcga agaggaagag acgcgcaccg aggcgatgaa gggatgggat aaataatcga
     4801 gaaagggtga gacgaatgat cgagccggta cgcagaatcg tcttcacgga ggatgagacg
     4861 cacgacgacg agcgcctgct ctcgcgcgag tggctggtga cgaacggcct cggcggcttc
     4921 gcctccggct ccatctcggg cgtgcccacg cggcgttacc acggcctcct catcgcggcc
     4981 ctgagcgcgc ccctcggccg catcgtcatg ctcaatcacc tctccgagca tgtccgcttc
     5041 agtgacggcc ggcgcgtcct cttcggcggg caggagcgtt cgggcggcaa gctccaactg
     5101 cccggcatcc actatctcaa ggagttccga ctcgaagccg gcctgccctt ctggcgcttc
     5161 gaggtggacg gctaccgcat cgagaagcgg gtgctgctgc cgcacatgca gaacacggtt
     5221 cacgtctctt acaaacttct cgcgggcgac ggccccgtgc gtctcaagct tcgcccggca
     5281 gtgcagttcc gcccgctcga agcgccggtc ggccagccgc acacgaagtc gtaccacttc
     5341 tcggcggacg accacctgat ggaactctcc ggcggcagcg acgaataccc gccgctccgt
     5401 ctgcgactct acggcgagcg cgccacgctc acgctccaca gcaagaagct ctcgaacgtc
     5461 ttctatcgcg tcgagcagcg tcgcggctac gaccacacgg ccgagcttta cagccccggc
     5521 tactttggcg tcagtctcga cgtggggcag gaggcgacgc tcgtcgcctc gaccgagtcg
     5581 tggcagaccg tcaacgcgct cgcacccgag gaggcgctgc ccgccgagtt cgagcgccgc
     5641 cggcgtctcg tcgccgccgc cccgacgccc gcgcgcgaag gcttcgccgc tgagttagtt
     5701 ctcgccgccg accagttcgt catcacgccg gccggccggc gcgaggacgc cgcccgcgcc
     5761 cgcgcctccg gcgaggaggc ccgctccgtc atcgccggct atcactggtt caccgactgg
     5821 ggccgcgaca cgatgatcag cttcgagggc ttgacgctga cgaccgggcg ccaccgcgag
     5881 gccgagtgga ttctccacac cttcgcccac tacgtccgcg acggtctcat ccccaacatg
     5941 ttccccgacg gcagcaacga gggcctctat cacaccgccg acgccacgct ctggtacttc
     6001 cacgccgttg accgctacct ccaaactacc ggcgaccgcg tcacactcga catcctcctg
     6061 cccaaactcc tcgacatcgt cgcgcaccac ttgaagggca cgcgcttccg tataggcgtc
     6121 gatgccgcgg acggactgct ccgtcagggc gaggagggct atcagctcac gtggatggac
     6181 gcgaagtgcg acggctgggt ggtcacgccg cgacgcggca aggccgtcga aatcaacgcg
     6241 ctctggtaca acgcgctgcg tctcacggaa gggtgggtgc gcgagacaca gggcgaggcg
     6301 gcggcgcgtc ccttccggga gcacgccgag cgcgcctacg aatctttcaa ccggcgcttc
     6361 tggtacgaag agggcgctca cctctacgac gtggtggata ccgaaggcgg cacggacgac
     6421 cccgcctgcc gccccaacca actcttcgcc ctctcgctcc gccaccccgt cctcgacccc
     6481 gcgcgctggg agtcagtgct cacggtcgtc cgcgacaaac tgctcacgcc cgtgggcctt
     6541 cgttcgctct cgcccgacca ccccgaatat cattccaagt atgacggcga cctgcgcgcg
     6601 cgcgacgccg catatcatca gggcaccgtt tggggctggc tcatcggccc ctacgtggac
     6661 gcgtggctcc gcgtgcgccc caacgaccgc gccggcgcgc gcgccctgct cgaaggcttc
     6721 cgcgcgcata tggacgaagc ctgcatcggc tccatcagcg aagtcttcga cgccgagccg
     6781 ccctacaccg cccgcggctg cgtcgcgcag gcgtggagcg tggccgaagt tttaaggtgc
     6841 tgggtgatga catcggagaa gtgatgagtg atgagtgata agtgataaga aagaacggag
     6901 gctattagct gttggctgtc agctaatcgc taagagctaa cagccaacag cttcttatca
     6961 ctcatcactt atcactcatc acctgtttta tatgagattc aaaagatcaa gcggtgtcct
     7021 cctccacccc acttccctgc ccggccgctt cggcgtcggc gacctcggcg cggcggcctt
     7081 gaagttcgtc gattggctcg ccgacgcggg gcagaccttc tggcagatca tgccgctcgg
     7141 ccccaccggc tacggcgact cgccctactc ttccctttcc gccttcgccg gcaacaccct
     7201 gctcatccgt cccgaacacc tcgtcgagcg cggcctgcta ggcgctgatg acttgaggga
     7261 cgcgcccgcc tcctcggccg cgcgcgtgga ctacggccga gtcatcgacc acaagcgcac
     7321 gctgctggag aaggcttaca aaaaattcaa atcaggctcg gacgcgggag cgcgcggcga
     7381 ctacgacggc ttcctccagt acgccgcctc gtggctcgac gactacgcgc tcttcgccgc
     7441 gctcagagac aaacacggcg gcgagtcgtg gcacacgtgg gaggcgggcg tggcgcggcg
     7501 agaggagggc gcgctcgcgc gcgctcgtga ggagttgcgc gagcaggtcg aggcccacaa
     7561 gtttttccag tatctcttct tcgagcagtg gctgcgtctg aaggcgcacg cacgcgagcg
     7621 cggcgtgtgg gtcgtcggcg acatgcccat cttcgtcgcg cacaactcgg cggacgtgtg
     7681 ggcgcggcca cacctcttca agctcgaaga agacggccgg ccgtcggcgg tcgccggtgt
     7741 cccgcccgat gccttcagca agaccgggca gctctggggc aaccctgtct acgactggga
     7801 gcggatgagt tcggagggat tcgtctggtg gacggagcgc gtgcgcgaga ccctgaagct
     7861 cgtggacgtt ttgcgtctcg atcacttccg cggcttcgcc gcctactggg aggtgcccgc
     7921 cggacacgag acggccgagc gcggccgttg ggtggacgca ccgggccgcg agcttttccg
     7981 tgcgctcaaa gatgccaacg gcggcaatga tctccccatc atcgccgaag acctcggcac
     8041 catcacgccg gacgtgcatg ccctgcgcga cgagttcgac ttccccggca tgcgcgtcct
     8101 ccagttcgcc ttcggcggcg acccgcgcga cacgcacctg ccgcacaact acacgcacaa
     8161 caccgtcgtc tacaccggca cgcacgacaa cgacacgacg ctgggctggc tgcgcgcccg
     8221 ctctgaggac gccgcggacg gcgacgaagg cttgcggcgc gagcgcgcca actgcctcaa
     8281 gtacctcgcg acggacggcg ccgagatcaa ctgggacttc atccgcgccg cccacgcctc
     8341 cgtcgccgac atcagcatcg tgcccctaca ggacgtgctc ggcctcgact ccgacgcccg
     8401 catgaacatc cccgccagca ccgaaggcaa ctgggcctgg cgcttccgcg aaggcgctct
     8461 caccgacgcc ttgcgcgacc gactccggga gatgactgag acctacggac gcctgccttc
     8521 acagtagtca tcgctaaccc tcttcacttt gtga
//
LOCUS       DASVES010000085         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_167216, whole genome shotgun sequence.
ACCESSION   DASVES010000085 DASVES010000000
VERSION     DASVES010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167216"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..2958
                     /locus_tag="VF240_03020"
     CDS             85..2958
                     /locus_tag="VF240_03020"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX7174245.1"
                     /translation="MNNPLREIMRLGQSVWYDNIRRAMLDGGDLLRKIEEDDLRGVTS
                     NPTIFEKAITGSADYDEQLGALTRAGRSAEEIYEELVAEDIGRAADIFRAVYEHTDAL
                     DGYISLEVNPRLAYDTDATIKEAERLFKRLGRKNVMIKIPAAQEGIPAIEESIRRGIN
                     INVTMIFSIENYEQVAEAFIRGLERRLDEGEPVDHIASVASFFVSRVDTSVDAELERM
                     ARDAGDADKRAMYESLLGRAGVANAKLAYQKFREIFHGERFAALRAEGAQVQRPLWAS
                     TGTKNPKYSDVLYVDNLIGPETVNTLPPATYTAVRDHGRAALTLEDGVEESRALFDRL
                     REVGIDLDAVTKKLQEDGLAAFVNSFDALLESIKAKRDAQLSGAGERLTASLGKHTEA
                     VNAALNESEAGDTVRRIWRKDAALWKEEAEHQKNIRNSLGWLTVVGEMKGVAGELKAF
                     ADHIRGEGFRHVIVCGMGGSSLCPEVLRRTFGRQEGYPELLVLDSTDPDAVAALGKQI
                     DVERTLFVIASKSGTTTEPLAFYKYWYAEVGKLKDKPGENFVAITDPGTKMEQDATRD
                     GFRRIFLNPSDIGGRYSALSYFGMLPAALMGIDAGKLLDRAERVVHACASAVAPAENP
                     GARLGAIIAECAKAGRDKLTIVADRRVESFGLWVEQLIAESTGKEGRGVVPVAGEPLG
                     SPLVYGDDRVFVSIAVGGVESEIESKLNALEAAGHPVIHRVLTDPYDLGEEFFLWEFA
                     TAFAGQRLGINPFDQPNVQESKDATREILEAYARDGRLREQDALVADGALTVYADEKT
                     RAALPSDSVAEVLRAHLARAGAGDYVALLNYFEETPESEKIIEAMRVRLRDATRCATT
                     AGYGPRFLHSTGQLHKGGPDKGVFLQLTAADAVDLPVPGERYTFGVLKQAQALGDFRS
                     LSSHGRRAIRIDLGADALAGLRRLEELITAALPRSGASSASGD"
     gene            2960..>4272
                     /gene="zwf"
                     /locus_tag="VF240_03025"
     CDS             2960..>4272
                     /gene="zwf"
                     /locus_tag="VF240_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7174246.1"
                     /translation="MPAANEAHVDENPLRTGLRLERTAEPCVVVIFGASGDLAKRKLI
                     PALYRLVQQRLLPAEFAVVGLGRTEMSEDEFRARMKEAVAEFSESKTVDEDLWASFAR
                     GLRYLTSDVANPADYTKLSEILAQVDRERGTQGNRLFYLSLPPDFYSETVKRLGAAGL
                     TKSEHGWVRVIVEKPFGTSLESARQLNAEILRHLDESQIFRIDHYLGKETVQNLFVFR
                     FANAIFEPLWNRQYIDHVQLTNAETVGVEGRGGYYERAGVVRDMIQNHVFQVLSLVAM
                     EPPASLQPDAIRDEKVKAMRAARVFTPERVREECARGQYGAGAVSGKPVAGYREEPGV
                     APDSQTETYAMVTMYFDNWRWADVPFYVRSGKRLPKRVTEIAIQFKAVPHQLFGPATG
                     RATPNQLIVRVQPDEGITMRFAAKVPGQATRVRDVNMDFRYGASFG"
BASE COUNT          793 a         1398 c         1394 g          687 t
ORIGIN      
        1 attgtttctg ttgaccgttg gcgactgaag ccgggcgtct ctctgcctac ttctttctgg
       61 cctcgggagt ttggagcgga gtcgatgaac aacccactgc gtgaaatcat gcggctcggc
      121 cagtccgtct ggtacgacaa catccggcgc gcgatgctcg acggtggcga cctgctccgt
      181 aagatcgagg aggacgacct gcgcggcgtc acttccaacc ccacgatctt cgagaaggcc
      241 atcaccggct ccgccgacta cgacgagcaa ctgggcgcgc tcacacgcgc cggcaggagc
      301 gccgaggaga tttatgagga actcgtcgcc gaagacatcg gccgcgccgc cgacatcttc
      361 cgcgccgtct acgaacacac cgacgccctc gacggctaca tcagcctcga agtaaatccc
      421 cggctcgcct acgacacgga cgccacgatc aaggaggccg agcgcctttt caagcgtctg
      481 ggccggaaga acgtgatgat caaaatcccc gccgcgcagg aggggatacc cgccatcgag
      541 gagtcgattc gcaggggcat caacatcaac gtcacgatga tcttctccat cgagaattac
      601 gagcaggtgg ccgaggcgtt tatccgcggg ctggagcggc gactcgacga gggcgaaccc
      661 gtagatcaca tcgcctcggt cgccagcttc ttcgtctcgc gtgtggacac ctccgtggac
      721 gccgagcttg agcgcatggc gcgcgatgcc ggcgacgctg ataagcgtgc gatgtacgag
      781 agtttgctcg gtcgtgcggg agtggcaaac gcgaagctcg cctaccagaa gtttagggag
      841 attttccacg gcgaacgctt cgccgcgttg cgcgcggaag gcgcgcaggt gcaacggccg
      901 ctgtgggcct cgaccggcac gaagaatccg aagtattcag acgtgctcta cgtcgataac
      961 ctcatcgggc cggagacggt caacactctc ccgccggcca cctacaccgc cgtccgcgat
     1021 cacggccgcg ccgcactcac gctcgaagac ggcgtcgaag aaagccgcgc actcttcgat
     1081 aggctgcgcg aagtcggcat cgaccttgac gccgtgacga agaaattaca ggaggacggg
     1141 ctggcggcct tcgtcaactc tttcgatgcg ctcctcgaat caatcaaagc caagcgcgac
     1201 gcgcagcttt cgggcgcagg cgagcgcctg acagcctcgc tcggcaaaca caccgaggcg
     1261 gtaaacgccg cgctcaatga atccgaagcc ggcgacacag tgcggcgcat ctggcgcaag
     1321 gacgcggcgc tctggaagga ggaggccgag catcagaaaa acatccgcaa ctcgctcgga
     1381 tggctgacgg tcgtcggcga gatgaaaggc gtcgcgggtg agttgaaggc gttcgccgac
     1441 cacatccgcg gcgagggctt ccgccacgtc atcgtctgcg ggatgggcgg gtcgtcgctc
     1501 tgccccgagg ttctgcgacg gactttcggg cggcaggagg gctacccgga actgctcgtg
     1561 ctcgactcga ccgacccgga cgccgtcgcc gcgctcggca agcagataga tgttgagcgc
     1621 actctcttcg tcatcgcgtc gaagtcgggc acgacgaccg agccactcgc gttctacaag
     1681 tactggtacg ccgaggtcgg gaaattgaaa gacaagcccg gcgagaactt cgtcgccatc
     1741 accgaccccg gcacgaagat ggagcaggac gccacgcgcg acggcttccg acgcatcttc
     1801 ctcaacccct ccgacatcgg cgggcgctac tccgcgctct cttatttcgg catgctgccg
     1861 gcggcgctga tgggcataga cgcgggcaag ctcctcgacc gcgccgagcg tgtggtgcat
     1921 gcgtgcgcgt cggccgtggc cccggccgag aaccccggcg cgcgtctggg cgcgatcatc
     1981 gccgagtgcg cgaaggcggg gcgcgacaag ctgacgattg tcgccgaccg gcgtgtcgaa
     2041 tcgttcggcc tctgggtgga gcagttgatt gcggagagca ccggcaagga ggggcgcggg
     2101 gtcgtgcccg tggcgggcga gccgctgggt agcccgctcg tatacggcga cgaccgcgtg
     2161 ttcgtctcca tcgccgtggg cggcgtcgag agcgagatcg agtcgaaact caatgcgctc
     2221 gaagcggcgg gacatcccgt aatccaccgc gtgctgacag acccttacga cctcggcgaa
     2281 gaattcttcc tgtgggaatt cgcgaccgcc ttcgccggcc aacgactcgg catcaacccc
     2341 ttcgaccagc cgaatgtgca ggagtcgaaa gatgcgacga gggaaatcct cgaagcctac
     2401 gcgcgcgacg gccgccttcg cgaacaggat gcgctcgtcg cggacggcgc gctgacagtc
     2461 tacgcggacg agaagacgcg ggcggcgctt ccttccgact ccgttgccga agttctccgc
     2521 gcacacctcg cgcgcgcggg cgcgggcgac tacgtcgcgc tgcttaatta tttcgaggag
     2581 acgccggaga gcgagaagat tattgaagca atgcgcgtgc gtctgcgcga cgcgacgcgc
     2641 tgcgcgacga ccgccggcta tgggccgcgc ttcctgcatt cgacggggca gctccacaag
     2701 ggtggaccgg acaaaggagt cttcctgcaa ctgaccgcgg ccgacgcggt tgacctcccc
     2761 gtccccggcg agcgttacac gttcggcgtg ctgaagcagg cgcaggcgct cggcgacttc
     2821 cgctcgctct cgtcacatgg ccggcgcgca atccgcatcg atctcggcgc agacgccctc
     2881 gccggtctcc gtcgcctcga agaactcatc accgcagcgc tcccgcgctc cggcgcgtca
     2941 agcgcatcag gcgattaaag tgcctgcggc gaatgaagca cacgtagacg agaaccccct
     3001 gcgcaccggg ctgcggctgg agcggacggc cgagccgtgt gtcgtcgtca tcttcggcgc
     3061 gtcgggcgat ctggcgaagc ggaaactcat ccccgcgctc taccggctcg tgcagcagag
     3121 gctcctgccg gcggagttcg ccgtcgtggg actcggacgc accgagatgt cggaggacga
     3181 gtttcgcgca cggatgaagg aagccgtcgc cgaattctcg gagtcgaaga cggtggacga
     3241 agacctctgg gcctcgttcg cgcgcgggct gcgatacctc acctcggacg tggcgaaccc
     3301 ggcggactac acaaagctct cggagatact cgcgcaagtg gatcgcgagc gcggcacgca
     3361 gggcaatcga ctcttctacc tgtcgctccc gcccgacttc tactccgaga cggtcaaacg
     3421 actcggcgcg gccgggctga cgaagtcgga gcacggctgg gtgcgcgtca tcgtcgagaa
     3481 acctttcggg acgagccttg agagcgcgcg acagttgaac gcggaaatcc ttcggcatct
     3541 cgacgagtcg caaatctttc gcatcgacca ctacctcggt aaggagaccg tccagaacct
     3601 cttcgtcttc cgcttcgcca acgctatctt cgagccgctg tggaatcggc agtacatcga
     3661 ccacgtgcag ttgacgaacg ccgagacggt cggcgtcgag gggcgcggcg gctactacga
     3721 acgcgcgggg gtggtgcgcg acatgatcca gaatcacgtc tttcaagtgc tctcgctcgt
     3781 ggcgatggag ccgcccgcgt cgctccagcc cgacgccatc cgcgacgaga aggtgaaggc
     3841 catgcgcgcg gcccgcgtgt tcacgcccga gcgcgtgcgc gaggagtgtg cgcgcggtca
     3901 gtacggcgca ggggccgtct cgggcaaacc tgtcgccggc tatcgagagg agccgggagt
     3961 cgcgcccgac tcccagaccg agacctacgc gatggtaacg atgtacttcg acaactggcg
     4021 ctgggcggac gtgccctttt acgtccgctc gggcaagcgt ctgccgaagc gcgttacgga
     4081 gatcgccatc cagttcaagg ccgtcccgca ccaactcttc ggcccggcga cggggcgggc
     4141 gacgccgaac cagcttatcg tccgcgtcca acccgacgaa ggcatcacca tgcgcttcgc
     4201 ggcaaaggtg ccgggtcagg ccacgcgcgt gcgcgacgtg aacatggact tccgctacgg
     4261 cgcgtccttc gg
//
LOCUS       DASVES010000086        14794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1407150, whole genome shotgun sequence.
ACCESSION   DASVES010000086 DASVES010000000
VERSION     DASVES010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14794
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407150"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="VF240_03030"
     CDS             <1..1941
                     /locus_tag="VF240_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7174247.1"
                     /translation="YLTEDTSLIRRQLEAGENLEYDPERPLMNNISTDEITPGWVCFY
                     YDETLGEYVYVGMREGAVKKDEVKGGGFSVVVSGLSKGCGSSRETAPFAERAAGVELV
                     IAKTIEKIYGQNCQNIGLLTSTDFGLIERIRSGEEIPLSEFTRGLDPVSQAIVEYGGL
                     FNYNKARLAGAVTPPAVETKARPMNIVEKIIARHAFVRAGEIGVEAVKPGDALFAVAD
                     VRFSHEYVTPMAESLFKGALGKTARVTEPESVYAFRDHLTFLGRVMSADKRKMGLLER
                     AEGLAVTQESFTDEQGIRLYGGTEERGSEAICHNAVVEDIALPGQLVIGTDSHTCMAG
                     VLGCFAFGVGSTDMANAWYTKDIRVKVPETVRFRLNGRKRADVSAKDVMLVILATAYM
                     KEGKGIGQVLEFAGEGIADWSIDERATLTNMAVEAGGFTGIIEPDERVTEYLVSMRGR
                     EADEIREGFLRSDPDAEYAASFDIDLSEIRPMVALPGDPRNGRPITELPEQVKIDAAY
                     GGSCTGGKKSDMDMYAEVLRSALEQGRRVAPGVHLYIQFGSQKIKEYARSRGYIELFE
                     RAGAELIDPSCGACINAGPGASPSAETVTVSAQNRNFPGRSGPGKLYLASPYVVAASA
                     VAGHIVEPAEFLGAPEEELVGA"
     gene            1938..2525
                     /locus_tag="VF240_03035"
     CDS             1938..2525
                     /locus_tag="VF240_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174248.1"
                     /translation="MRGIIGTGRVGQTHSEKYAREEFERRFLLRALPEGLTKADPHTQ
                     IIDNYITSTRLRLRKVRVPATNEWTLKLTQKFAPDPADFSRTLITNIYLTEYEYEVLS
                     VFEANELRKNRYPYEYDGRRYTVDVFLGQLLGLILAETEFTNEAEMRALEAPSFAARE
                     VTSDEMFTGARLVELTAEEIRAHLRQRGEGDEPPS"
     gene            2737..3885
                     /locus_tag="VF240_03040"
     CDS             2737..3885
                     /locus_tag="VF240_03040"
                     /inference="COORDINATES: protein motif:HMM:NF023166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HEX7174249.1"
                     /translation="MMINFHRKLFLLACVLLIVACGDARTFEAAPQQTASQTASPAPT
                     ATPRPQPAAAQTFRGQIGDKLRVRMRLRRDGDSLSGSYFYEGRKGELSLKGSVDGRGN
                     FTLREQTAAGAQSGIFKGKWGTNETGETELTGDWSRPDGSRATAFTLTELPVELASGA
                     AVVTREIKEEKKRPRYTIGAEYPQIEGAAGGRFDGFNRAVAAVAAGEANDFRKGMAEW
                     EDTPGSEIGSYIEAGYSFGVATDDLVSVLFDIGDYYSGAAHPNHHTRAVNYDVRRGRV
                     LDLAELFKPSALYLKTISDYCVKDLKRQFREGGWSEPEQMDAGIEEGAAPNAENYQSW
                     LVTRRGLAITFDPYRVAPYAAGPQSVFIPYSALREIIDPDGPLAPFVK"
     gene            3920..4399
                     /locus_tag="VF240_03045"
     CDS             3920..4399
                     /locus_tag="VF240_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX7174250.1"
                     /translation="MTSPVNIYTVGHGRHPFAHFLGLLQTHGVELVCDVRSVARSRWP
                     QYNGRVLEELLKENGIGYEWLPECGGKMPAPPAEFARGLERVMELASDARVALMCSES
                     RPLTQHRPQPRANCHRVGLLSPPLRARGARLIHILPDGDTLEMDEATLPSIWRDDWQ"
     gene            4476..5606
                     /locus_tag="VF240_03050"
     CDS             4476..5606
                     /locus_tag="VF240_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7174251.1"
                     /translation="MTTEKEQSEVEHLKEQPEVQKALKQTGGAAEKAAAEQQEESPPV
                     ETRHKVWIGTYVIALLALGGLYYGLRLNALGIGEQLAAAGFVEQYWWLERLRPLLIRL
                     TLGAVAVTVTLAAAKFVDSYVIGRLNDAPTEFNLRRVLRLVVVIVLLLIGLSVLFANW
                     YTAVVSIGLLSLILGFALQTPITSLIGWIYILVRTPYRVGDRIRIGEATGDVIDVGYL
                     DTTLWEFGGQYLSTDHPSGRIIKFPNSDALSNPVYNYSWPLFPYIWNEVKFQVAYDSD
                     FEFIARTMQEVTEAELGESMMENVKIFRELLAQTPVDRLEVRERPAVFFRVGDNTWIE
                     AIVRYLVYPRDAGRVKNRLIETLLKRLNAEPERVKFPKSNMR"
     gene            complement(5699..6958)
                     /locus_tag="VF240_03055"
     CDS             complement(5699..6958)
                     /locus_tag="VF240_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7174252.1"
                     /translation="MKRSPLFVIFVTVFIDLVGFGIVIPILPYYVESFGGTPRQQGLL
                     LTSYSVMQLIFSPILGRMSDKYGRRPVLFLSLVGSGVGFLIMGFAGSLWMLFAGRILA
                     GITGGNISTAQAYIADVTTPENRAKGMGMFGAAFGLGFIFGPAIGGIMGWWLGIHAPV
                     IFAGLLALANAALLYFTLPETITPDHPARTTVRGWGHFFQQLRQRRLAIVLLVYFLFV
                     VSFSIMTTTFIYYTILRYDYNARDNGYLFAYIGVLAAVVQGLLIGRLVKRWGEAALVI
                     AGAFVLTASLALLPYVGRPESGWHGGVWGLLALLACFSLGNSLATPSLSSLASKSVGP
                     AEQGGVLGATQSAASLARAVGPYLGGVLIYSAAAPRGFDDHTIFVTFWAAAGIMFAAL
                     LLSAYFARAHAPTPAPAGAGILEEASG"
     gene            complement(7034..7486)
                     /locus_tag="VF240_03060"
     CDS             complement(7034..7486)
                     /locus_tag="VF240_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7174253.1"
                     /translation="MFQKLIRTSRVWFTLPLRLALGAVFVAHGAQKVLGLWGGPGLAQ
                     FASGQAPLGLKPAWFWLGAAALAELVGGALVLMGLMTRLGALLLLSVMLVAMFGVHWT
                     GGFFITNQPMPGIEYTLALAGMALALLVSGGGRLSLDEALMDPRYRRR"
     gene            7869..9080
                     /locus_tag="VF240_03065"
     CDS             7869..9080
                     /locus_tag="VF240_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174254.1"
                     /translation="MTTIRLILLIAALFAAPQLMCEAVAAAQAQKTPTTRPAPTGLAL
                     EVTYLKGRAPGYDAVPPTGPAPRGTWYGLFGRLPDWRPAEGSLPTRAVNVVSRLEGDH
                     VRVVVSVHQGERFHEKEAFVSTHLVRVNEGVEVGELTRFGVEPFTIKVVRVGPLPTNP
                     PALVSRAPSIVLTGALPAADTTLPTYRVSVQNLSAKDVVALGVEVRVGGEQQLSAIRQ
                     GREGERLMAGGGAYELELLGVNEARPTAEGYAPAAPPDQEVRVTAAVFADGSFEGDAR
                     TAARFRAFVYGRRAQLARLVPLLREALAGANNVDGAAAAKRFHERVSALDDRAEESDV
                     KALWKEFPTLDRGTDLKQTIEITMNGVKREVLREVEKFALSEIEQPGAGDFRAWLGAS
                     KERYERWLARL"
     gene            9089..9637
                     /locus_tag="VF240_03070"
     CDS             9089..9637
                     /locus_tag="VF240_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174255.1"
                     /translation="MSRNAFAAALLCALVLCAAAGAPAQSTLNDDEAGVPRGELSEIR
                     DKRRALLIVNRSYSVDTRGPLRGVVEEVFDKEGRVALRHEYAHDLVSRRLERYEREGG
                     LKIVETLEEADFVIVYKVVALHRSFSEDEPFVYGEMFVFLNRTPERPQPVLLWRTKGD
                     HTTPGEAAGDFLKSLKAVRGEK"
     gene            9677..10201
                     /locus_tag="VF240_03075"
     CDS             9677..10201
                     /locus_tag="VF240_03075"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX7174256.1"
                     /translation="MLLLKTLLHVLLLPCTLIVWAPLLLVGGWRGAVSGARGGAPAVI
                     GAALIVCGLAVFVWCHRDFLTKGRGTPNPLDPPKFLVARGPYRWVRNPMYVCAILMLF
                     GEALIFRSPAFLLYALVVLLGFHLFVMLYEEPTLRRLFGASYEDYCRTVPRWLPRRPA
                     HHSKPAPPPAASVL"
     gene            10250..10501
                     /locus_tag="VF240_03080"
     CDS             10250..10501
                     /locus_tag="VF240_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174257.1"
                     /translation="MSAELKVTLPDGLAREAEANGLLTAEAIEELLRAELQRRRVGKL
                     FAAAERLVEAGAPPLTEAEVEAEIAAARRERRAPDASRS"
     gene            10724..14350
                     /locus_tag="VF240_03085"
     CDS             10724..14350
                     /locus_tag="VF240_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7174258.1"
                     /translation="MTELIIVGLVVLCLLLLGVVWYFVLGFRVRETGGELERVRAELK
                     EARATLEELRDARREIREAVEQARSVAQQSAADAQTTADAAEGADSLAELHAADANAA
                     AAAPEPDSNDVFSLAWAMDDYFNRSAYPSDLLVNEHFQRGVEILKGPAYSASDLLSYA
                     RGGNVVVSCMALEALSERENSEGVVFLLIDYINGLSYWPRFYAMRALAERAGPLPMVT
                     VLLLRLNATWTDYISLNALRDFLTRRMEIGEEARFGNLLSGLDEERSAALSALMGELR
                     GYVPAEMHEEFERWQRERVDTEFLKSFGRVWDGVGAGRGAEDGVVMLPAVEERLTEML
                     TALGKDPPRSVMLVGESGTGKTTLTRVLARRLQREGWTIFEASAADLIAGQKYIGEIE
                     GRAQTFVRQIGGKRVLWVVPSFQELLWAGRHQYSPTGVLDLVMPQIESGAIKVVGELT
                     SVAYENLVRQRPKLRAAMQTVRVAPMPDAETFELGRRWAELHAPAEGDDDHNARVSPE
                     TLREAFQLTKQYLNDKAAPGNLILLLDSTHRRLVTEQGANRGQISLDDLLVTLSQLTG
                     LPTQILDDRAGLDLSELRAFFERRVMGQTEAVDCLVERVAMVKAGLTDPTRPQGVFLF
                     VGPTGTGKTEIAKTLAEFLFGSPERLIRLDMSEFQTSETLDRILGETTDAGDSTALVN
                     SIRKQPFSVILLDEFEKAHPNVWDLFLQVFDDGRLTDRRGATADFRHCVLIMTSNLGA
                     TLPTGASIGFSHEQAQFTSGAVERAVGRSFRREFINRIDRVVVFRPLSLSAMRDILRK
                     ELNDVLGRRGFRTRQWAVEWDESAIDFLLQKGFTADLGARPLKRSVERYLLSPLSLTI
                     VNHQFPEGDQFLLVRAGGDDSIEVEFVDPDAPESPPAAASVIDEEEAVEESAERRLES
                     IALDARGTGAEVSLLQSKYDELRAQVDSDEWQQRKQDALLQTARPGFWDSPERFGVLG
                     LAEYMDRIEAGLDTAGSLLARLTGGRHRADERRAYLPDLVERVAQQLYLVDAACCGMG
                     AGAARDAFLLVAAVRETGADAPAADRFARRVARMYRRWAERRRMRFEVLEESGGEGTG
                     GAGAYRLSAAVSGFAAYSILQAEAGLHVLETPQRERSTFNRASAQVRIVAQPEEPAGR
                     APDALRRQAEAALEQDASAQSAANTIVRRYREEPSPLVRDSVRGWRTGKLERVLDGDF
                     DLFR"
     gene            14498..>14794
                     /locus_tag="VF240_03090"
     CDS             14498..>14794
                     /locus_tag="VF240_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174259.1"
                     /translation="MNRQRLLSWLLRLAGTFEMLAFISVVMPRAWMEASHAWLGLGEM
                     PGGPVLIFMIRQASYSYGMHGISLWILATDVKRFRPLVVFNAVAFLLAAPVFFII"
BASE COUNT         2601 a         4984 c         4868 g         2341 t
ORIGIN      
        1 tatctgaccg aggacacgtc cctcatccgc cgccagctcg aagcgggcga aaacctcgaa
       61 tacgaccccg agcgccccct gatgaacaac atctcgaccg acgagatcac gcccggctgg
      121 gtctgcttct actacgacga aacgctcggc gagtatgtct acgtcggcat gcgcgagggg
      181 gcggtgaaga aggacgaggt caaagggggc ggcttctccg tcgtcgtctc cggtctctcg
      241 aagggctgcg gctcctcgcg cgagaccgcg cccttcgccg agcgcgcggc cggcgtggaa
      301 ctcgtcatcg ccaaaaccat cgagaagatt tacggacaga actgccagaa catcggcctg
      361 ctgacctcca ccgatttcgg gctgatcgaa cgcatccgaa gcggcgaaga gattccgctc
      421 tcggagttca cacgcggcct cgaccccgtc tcgcaagcaa tcgtcgaata cggcggtctc
      481 ttcaactaca acaaggctcg cctcgccggc gcggtcacgc cgcccgccgt cgagaccaaa
      541 gcgcggccaa tgaacatcgt cgagaagatc atcgcgcgcc acgcgtttgt gcgcgcgggg
      601 gaaatcggcg tcgaagcagt caagcccgga gacgcgctct tcgctgtcgc ggacgtgcgc
      661 ttctcacacg agtacgtcac gccgatggcc gaaagtctct tcaagggcgc gctgggcaaa
      721 acggcgcgcg tcaccgagcc ggaatcggtc tacgccttcc gcgaccacct caccttcctc
      781 ggccgcgtga tgtcggccga caaacgcaag atggggctgc tcgaacgtgc cgaggggctg
      841 gccgtgacgc aggagagttt taccgacgag cagggcatac gcctctacgg cggcacagag
      901 gagcgcggct ccgaagccat ctgccacaac gccgtcgttg aagacatcgc gttgccgggt
      961 cagctcgtca tcgggacgga ctcgcacacc tgcatggccg gcgtgctcgg ctgcttcgcc
     1021 ttcggcgtcg gctccaccga catggccaac gcctggtaca cgaaagacat tcgcgtcaaa
     1081 gtaccggaga ccgtccgctt ccgtctcaat ggccgcaagc gcgccgacgt gtcggccaaa
     1141 gacgtgatgc tcgtcatcct tgcgaccgcc tacatgaagg aaggtaaagg catcggacaa
     1201 gtcttggagt tcgctggcga aggcatcgcc gactggtcga ttgacgagcg cgccacgctc
     1261 accaacatgg ccgtggaggc cggcggcttt accggcatca tcgaaccgga cgagcgcgtc
     1321 accgaatacc tcgtctcgat gcgcggccgc gaagccgacg agattcgaga aggctttctc
     1381 cgcagcgacc ccgacgccga gtacgccgcc agcttcgaca tcgacctctc cgagattcgc
     1441 ccgatggtgg ccctgcccgg cgacccgcgc aacggccgcc ccatcaccga gcttcccgaa
     1501 caggtcaaaa tcgacgccgc ttacggcggc tcctgcaccg gcggcaagaa gtcagacatg
     1561 gacatgtacg ccgaggtgct ccgaagcgcg ctcgaacagg gccgacgcgt cgcgcccggc
     1621 gtccatctct acatccagtt cggctcgcag aagatcaagg agtacgcgcg ctctcgcggc
     1681 tacatcgaac tcttcgagcg cgccggcgcg gagctgatcg accccagttg cggcgcgtgc
     1741 atcaacgccg gccccggagc ctcgccctcg gccgagacgg tgacggtgag cgcgcagaac
     1801 cgcaacttcc ccggccgcag cggccccggc aagctctacc tcgccagtcc ttacgtcgtc
     1861 gccgcgtcgg ccgtcgccgg tcacatcgtc gagccggccg agtttttggg cgcgcccgag
     1921 gaggagttgg tcggcgcatg aggggcatca tcggaacggg acgcgtcggc cagacgcatt
     1981 cggagaagta cgcgcgcgaa gagttcgagc ggcgcttcct gttgcgcgct ctgcccgagg
     2041 gcttgacgaa ggccgacccg cacacgcaga tcatcgacaa ctacatcacg agcacgcgcc
     2101 tgcgcctgcg caaggtgcgc gttcccgcga cgaacgagtg gacgctcaag ctcacgcaga
     2161 agttcgctcc cgaccccgcg gacttctcgc gcacgttgat tacgaacatc tacctcacgg
     2221 agtatgagta tgaagtgctc tccgtcttcg aggccaacga gctacgcaag aaccgctacc
     2281 cttacgagta cgacggacgc aggtacacgg tggatgtttt cctcggccag ctcctcggac
     2341 tcatcctcgc ggagacggag ttcacgaacg aagcggagat gcgcgcctta gaagcgccgt
     2401 cgttcgccgc ccgcgaggtg acgagcgacg agatgttcac cggcgcgcgt ctcgtcgagc
     2461 tgaccgcaga ggaaatccgc gcacacttgc ggcagcgcgg cgagggcgac gagccgccaa
     2521 gttgactgta ggggccgcgg acaagtccgc tgcccgcgcc gtgggaaaca attaacatgg
     2581 attgacagga tgagcaggat gagatgattt gaaatctcaa atctcaaatc ttgaatctga
     2641 aatctcaact cagcactttc ttcatcttgc tcatcctgcc tatccatgtt aattgctctg
     2701 taattgtcgg gcagcggact tgtccgcggc ccctacatga tgattaattt ccatagaaaa
     2761 ctttttctgc tcgcatgcgt gctgctaatc gtcgcgtgcg gcgacgcgcg cacgttcgag
     2821 gccgcgccgc agcagaccgc gagtcagacg gcctcacccg caccgaccgc gacgccacgg
     2881 ccgcagcccg cagcggcgca aaccttccgc ggccagatcg gcgacaaact gcgcgtgcgg
     2941 atgcgcctcc ggcgcgacgg cgactcgctc tccggctctt acttttacga gggccgcaaa
     3001 ggtgaactct ccctcaaagg ctccgtggac ggccgaggca acttcacgtt gcgcgagcag
     3061 acggccgccg gcgcgcagag cggcatcttc aagggcaagt ggggcacgaa cgagacggga
     3121 gagacggagc taaccggcga ctggtcgcgg cccgacggct cgcgcgcgac cgccttcacg
     3181 ctcacagagc ttccggtcga gctagccagc ggcgccgccg tcgtcacgcg cgagattaaa
     3241 gaagagaaga agcggccgcg ctacaccatc ggggccgagt acccgcagat cgaaggggcg
     3301 gcgggcggtc gcttcgacgg cttcaaccgg gcggtcgcgg ccgtggccgc cggcgaggcg
     3361 aacgacttca ggaaggggat ggccgagtgg gaggacacgc ccggctccga gataggcagc
     3421 tacatcgagg cgggctactc gttcggcgtc gccaccgacg acttagtcag cgtgctcttc
     3481 gacatcggcg attactacag cggcgccgcg caccccaacc accatacgcg agcggtcaac
     3541 tacgacgtgc gtcggggccg cgtgctcgac ctcgcggaac tcttcaagcc ctcggccctc
     3601 tatctcaaga ccatctccga ctactgcgtc aaagacctca agcggcagtt ccgcgagggc
     3661 ggctggagcg agccggagca gatggacgcc ggcatcgagg agggagccgc gccgaacgcg
     3721 gagaactatc agagctggct cgtcacgcgc cgggggctgg ccatcacctt cgacccctac
     3781 cgggtcgccc cctacgcggc ggggccgcaa agcgtcttca tcccctactc cgccctgcgc
     3841 gaaatcatcg accccgacgg gccgctcgcc ccgttcgtga agtgagccga caaacttaac
     3901 ttcatatttc agattcgcga tgacctctcc cgtcaacatc tacaccgtcg gccacggccg
     3961 ccacccgttc gcgcacttcc tcggtctgtt gcaaacgcac ggcgtcgaac tcgtctgcga
     4021 cgtgcgcagc gtggcgcgct cgcgctggcc gcagtacaac gggcgcgtgc tcgaagagct
     4081 gctcaaagag aacggcatcg gctacgagtg gctgcccgag tgcggcggca agatgccggc
     4141 gccgcccgcg gagttcgcgc gggggctgga gcgcgtcatg gagctggcgt cggacgcgcg
     4201 cgtggcgctg atgtgctcgg agtcgcgccc gctcacacag caccgcccgc agccgcgggc
     4261 caactgccac cgcgtcggcc tgctctcgcc ccccctgcgc gcacgcggcg cacgtctcat
     4321 acatatcctg cccgacggcg acacactgga gatggacgag gcgacgctgc cgtcaatctg
     4381 gagagacgac tggcagtaga cacgcgcgcg acttccgcga gttcagtttc acccggccac
     4441 ggtcacaaac agttggagaa cggcggagac ttcagatgac aacggagaag gagcagagcg
     4501 aagtcgaaca cctcaaggag cagcccgagg tgcagaaggc gctcaagcag acgggcggcg
     4561 ccgccgagaa agccgcggcc gagcagcagg aggagtcgcc gcccgtcgag acgcgccaca
     4621 aggtctggat cggcacttac gtcatcgcgc tgctcgccct cggcggcctc tattacgggc
     4681 tgcgcttaaa cgcgctcggc atcggcgagc agttggccgc ggccggcttc gtcgaacagt
     4741 actggtggct ggagagactg cggccgctgc tcatacggct cacgctcggc gcggtggccg
     4801 tcacggtcac gctggccgcg gcgaagttcg ttgactctta cgtcatcggc cgcctgaatg
     4861 atgcgccgac ggagttcaac ctccgccgcg tgctgcgtct ggtcgtcgtc atcgtcttgc
     4921 tgctcatcgg actgtcggtg ctcttcgcca actggtacac ggccgtcgtc agcatcgggc
     4981 tgctctcgct catcctcggc ttcgccctcc agacgccgat cacgagtctc atcggctgga
     5041 tttacatcct cgtccgcacg ccctaccgcg tcggcgaccg cattcgcatc ggcgaggcga
     5101 cgggcgacgt aatcgacgtg ggctacctcg acaccacgct ctgggagttt ggcgggcagt
     5161 atctttcgac cgaccacccg tcgggccgca tcatcaagtt tccgaactcc gacgcgctct
     5221 cgaaccccgt ctacaactac tcttggccgc tcttccccta catctggaac gaggtcaagt
     5281 ttcaggtcgc ctacgacagc gacttcgagt tcatcgcgcg cacgatgcag gaggtgacgg
     5341 aggcggagct gggcgagtcg atgatggaga acgtgaaaat cttccgcgag cttttggcgc
     5401 agacgcccgt tgaccggctg gaggtgcgcg agcgcccggc ggtcttcttc cgcgtcggcg
     5461 acaacacctg gatcgaggcc atcgtccgct acctcgtcta cccgcgcgat gccggccgcg
     5521 tcaagaaccg cctcatcgag accctgctca aacgcctcaa cgccgaaccc gagcgcgtca
     5581 agttccccaa gagcaacatg cgctaaagaa ggcaaaagta aaaaggcaaa aggcaaaagt
     5641 gaagaggcga ctcggccttc ccacttttgc cttttgcctt tttacttttg ccttgtcctc
     5701 acccgctcgc ttcttcgagg atgcccgcgc cggcgggggc gggcgtgggg gcatgggcgc
     5761 gggcgaagta ggctgagagc aggagggcgg cgaacatgat gccggcggcg gcccagaagg
     5821 tgacgaagat ggtgtggtcg tcgaagccgc ggggggcggc ggcgctgtag atgagcacgc
     5881 cgccgaggta ggggccgacg gcgcgggcga ggctcgccgc cgactgcgtc gcgcccagca
     5941 cgccgccctg ctcggccggc ccgaccgact tcgacgcgag gcttgagagc gaaggcgtcg
     6001 ccagcgagtt ccccagagag aagcaggcga ggagcgcgag caggccccac accccgccgt
     6061 gccagcccga ctccggccgc cccacgtagg gcagcaacgc cagactcgcc gtcagcacga
     6121 acgcccccgc gatgacgagc gccgcctcgc cccaacgctt caccagacgg ccgatgagca
     6181 atccctgcac gacggccgcg agcacgccga tgtaggcgaa gaggtagccg ttgtcgcgcg
     6241 cgttgtagtc gtagcgcaga atcgtgtagt agatgaaggt cgtggtcatg atcgagaaag
     6301 agaccacgaa gaggaagtag acgagcagca cgatggcgag ccgtcgctgg cggagttgtt
     6361 ggaagaagtg gccccagccg cgaacggtcg tgcgcgccgg atggtcgggc gtgatggtct
     6421 cgggcagggt gaagtagagc aaggcggcgt tagcgagcgc gagcaggccg gcgaagatga
     6481 cgggcgcgtg gatgccgagc caccagccca tgatgccgcc gatggccggg ccgaagatga
     6541 agccgaggcc gaaggccgcg ccgaacatcc ccatgccctt cgcgcggttc tcgggcgtcg
     6601 tcacgtcggc gatgtaggcc tgcgccgtcg agatgttgcc gcccgtgatg ccggcgagga
     6661 tgcggcccgc aaagagcatc cacagcgagc cggcgaatcc catgatgaga aaccccacgc
     6721 ctgacccgac gaggctcagg aagaggacgg gccgccgccc gtacttgtcc gacatccgcc
     6781 cgaggatggg cgagaagatg agctgcatca ccgagtagga cgtgagcagc aggccctgct
     6841 gccggggcgt gccgccgaag ctctcgacat agtacggcag gatggggatg acgatgccga
     6901 agccgacgag gtcgataaag accgtgacga agatgacgaa gagcggagag cgtttcataa
     6961 agtgatgagt gatgagtgat aagtgataag aggaagacaa ggttttgtct tatcactcat
     7021 cactcatcac ttatcaccgt cttctatatc tggggtccat cagcgcctcg tcgagcgaga
     7081 gtcggccgcc gccggagacg agaagggcga gggccatccc cgccagcgcc agcgtgtatt
     7141 cgatgccggg catgggctgg ttggtgatga agaagccgcc cgtccagtgg acgccgaaca
     7201 tcgcgacgag catcacgctc aggagcagga gcgcaccgag gcgcgtcatc aatcccatga
     7261 ggacgagcgc gccgcccacg agttccgcca gcgccgccgc gcccagccag aaccacgccg
     7321 gcttgagtcc caacggcgcc tgccccgaag cgaactgcgc cagaccgggg ccgccccaca
     7381 gccccaaaac tttctgcgcg ccgtgcgcga cgaagacggc cccgagcgcg aggcgcaggg
     7441 ggagcgtgaa ccagacgcgc gaggtgcgga tcagcttttg aaacatcgac gcgtgacctc
     7501 tccttgagct tcggccgtgt ccctcccgaa cacggcagtt ttaccacagc cccgcatacg
     7561 cacgccacgc acgccgcgcc gcacccgcgc ctccgcggcc cgctcgttac agagccgggg
     7621 aacacggatg agacggatgg cggacagatc gtcacggatt ccaatccgtc tttatccgtt
     7681 tcaatccgtc ccatccgtgt tccccccacg ccgtcgcgcg cgctcagcga ctcgaacgaa
     7741 acgcactctc gaaagaggcc gacggtttgg agacgataac gcatgccgtt gacgagaggc
     7801 cgggctttaa cgtaaaagca tcaacatccc cacgcgcccg cgccgccgcc ggacacggag
     7861 aaaagcagat gaccacaatc cgactcatcc tcctcatcgc cgccctgttc gccgcgcccc
     7921 aacttatgtg cgaggccgtc gccgccgcgc aggcgcagaa gacgccgacg acgcgccccg
     7981 cgcccaccgg cctcgcgctg gaagtcacct acctcaaggg tcgtgcgccc ggttacgacg
     8041 cggtgccccc gaccggcccc gcgccgcgcg gcacatggta cggcctcttc ggacgcctgc
     8101 ccgactggcg acccgccgaa ggctcgctgc cgacgcgggc cgtcaacgtc gtctcccggc
     8161 tggagggcga ccacgtgcgc gtcgtcgtct ccgtgcatca gggcgaacgg ttccacgaga
     8221 aggaggcgtt cgtctccacc cacctcgtgc gcgtgaacga aggcgtcgag gtcggcgagc
     8281 tgacgcggtt cggcgtcgag ccgttcacga tcaaggtcgt ccgggtcggg ccgctgccga
     8341 cgaacccgcc cgcgctggtg agccgcgcgc cgtccatcgt cctcaccggc gccctgcccg
     8401 ccgccgacac cacgctcccc acataccgag tgtccgtgca gaatctctcc gcgaaggacg
     8461 tggtcgcgct cggcgtcgag gtgcgcgtgg gcggcgagca gcagctcagc gcgatccggc
     8521 aaggccgcga aggcgagcgt ctgatggcgg gcggcggcgc gtatgagtta gaactgctgg
     8581 gcgtgaacga ggcgcggccg acggccgagg ggtacgcgcc cgccgcgccg cccgaccagg
     8641 aggtgcgcgt cacggccgcc gtcttcgccg acggctcctt cgagggcgac gcccgcaccg
     8701 ccgccagatt ccgcgccttc gtctacgggc ggcgggcgca gttggcccgc ctcgtcccgc
     8761 tgttgcggga ggcgcttgcc ggggcgaaca acgtggacgg cgcggcggcc gcgaagaggt
     8821 ttcacgagcg cgtctcggcg ctcgacgaca gggccgagga gtcggacgtg aaggcgctgt
     8881 ggaaagagtt cccgaccctc gaccggggca cggacctgaa gcagacgatt gaaatcacga
     8941 tgaacggcgt caagcgcgag gtgctcagag aggtggaaaa attcgccctg tccgagatag
     9001 agcagccggg cgcaggcgac ttccgcgcgt ggctcggggc gagcaaggag aggtacgaga
     9061 ggtggctcgc gaggctctga agattcacat gtcgcgaaac gccttcgcgg ccgccctgct
     9121 ctgtgccctc gtgctgtgcg cggcggcggg cgcgccggcg cagtcaacac taaatgacga
     9181 cgaggccggg gtgccgcgcg gcgagctgtc ggagatcagg gacaagcggc gcgcgctgct
     9241 catcgtcaac cgtagttatt cggtagacac gcgcgggccg ctgcgcggtg tggtcgaaga
     9301 agtgttcgat aaggaggggc gcgtcgccct gcgccatgag tatgcacacg acctcgtctc
     9361 ccgaaggctc gaacgctacg agcgcgaggg cgggctgaaa atcgtcgaga cgttggagga
     9421 ggctgacttc gtcatcgtct acaaggtcgt cgcgctgcac cgctccttct ccgaagacga
     9481 gcctttcgtc tacggcgaga tgttcgtctt cctgaaccgg acgccggagc ggccgcagcc
     9541 cgtcctgctg tggcggacga agggcgacca caccacgccg ggcgaggccg ccggcgactt
     9601 cctcaaatca ctcaaggccg tgcgcgggga gaagtgaccg cgcgcgcgcg actcgaccga
     9661 caccaacaat agacccatgc ttctcctcaa gacgcttctg cacgtgctgc tcctgccgtg
     9721 tacgctcatc gtctgggcgc cgctgctgct cgtgggcggc tggcgcgggg ccgtgagcgg
     9781 cgcacgcggc ggggcgccgg ccgtcatcgg cgcggcgctc atcgtttgtg gcctcgccgt
     9841 gttcgtctgg tgccaccgcg attttctgac aaaggggcgc ggcacgccga acccgctcga
     9901 cccgccgaaa ttcctggtcg cgcgcgggcc ttatcgctgg gtacgcaatc cgatgtacgt
     9961 ctgcgcgatt ctcatgctct tcggcgaagc tctcatcttc cgctcccctg cgtttctcct
    10021 ctacgcgctc gtcgtcctcc tcggcttcca cctcttcgtc atgctctacg aggagccgac
    10081 tttgcgccgg ctcttcggcg cgtcctacga agactactgc cgcaccgtgc cgcgctggct
    10141 gccgcgccgt cccgctcatc actcgaagcc cgcaccgccg cccgcggcct ccgtgctctg
    10201 acgcctcccg gatgtgctac catcttcgcc gaaggagcgg gtgcaaacga tgtcggccga
    10261 gttaaaagtt acattacctg acggactggc gcgcgaggcc gaggccaatg gcctgctcac
    10321 ggccgaggcc atcgaggagc tgctgcgcgc ggagctacag cgccggcgcg tcggcaagtt
    10381 gttcgcggcg gccgagcggt tggtggaggc gggcgcgccc ccgctgacgg aggcggaggt
    10441 cgaagccgaa atcgccgccg cccggcgcga acggcgtgcg ccggatgcga gtcgtagctg
    10501 aaacgaaagc cggagaagat cgttcccacc gtcattgaag accccgacga cgacaacgtc
    10561 ttagcctgtg ccgtcgcggc ggggtgtgag gtcatcgtct cgggcgacag ccacctgctg
    10621 cgtctgaaac aatacgaaca actccgtatt ctcacggcgg ccgaactgct gtcagaaatt
    10681 aaggtgtcgt gattagtcag acgccgcgcg ccctcgtcag ccgatgacgg aactcatcat
    10741 cgttggactc gtcgtgttgt gcctgctgct gctgggcgtg gtgtggtact tcgtgctcgg
    10801 cttccgggtg agggagaccg ggggcgaatt agagcgcgtg cgcgctgagc tgaaggaggc
    10861 gcgcgcgacg ctcgaagagt tgagggacgc ccgccgggag attcgtgagg cggtcgagca
    10921 ggcgcgctcc gtcgcgcaac agagcgcggc cgacgcgcag acgacggccg acgccgcgga
    10981 gggcgcggac tcgctggcgg aactgcacgc ggccgacgca aacgctgccg ccgccgcgcc
    11041 cgagccggac tcgaacgacg tgttctcact cgcgtgggcg atggacgatt acttcaaccg
    11101 cagcgcctac ccgagcgatc tgctcgtcaa cgagcacttc cagcggggcg tggaaatttt
    11161 gaaaggcccg gcttactcgg ccagcgacct gctctcctac gcgcgcggcg gcaacgtcgt
    11221 cgtctcctgc atggcgctcg aagctctctc cgagcgcgag aacagcgaag gcgtagtctt
    11281 cctcctcatc gactacatca acgggctttc ctactggccg cgcttctacg ccatgcgcgc
    11341 tctcgccgag cgcgccggcc cgctccccat ggtcaccgta ctgctgctgc gcctcaacgc
    11401 gacctggacg gactacatct cgctcaacgc cctgcgcgac ttcctcacgc gccgcatgga
    11461 gatcggcgag gaagcgcgct tcggcaatct gctgagcggc ctcgacgagg agcggtcggc
    11521 cgcgctgtcg gccctgatgg gcgagctgcg cggctacgtg cccgcggaga tgcacgagga
    11581 gttcgagcgt tggcagcggg agcgcgtgga cacggagttt ttgaagtcgt tcgggcgcgt
    11641 gtgggatggc gtcggcgcgg gccgcggcgc ggaggacggc gtcgtcatgc tcccggcggt
    11701 cgaggagcgt ttgacggaga tgttgacggc gctcggcaaa gacccgccgc gctcggtgat
    11761 gctcgtgggc gagtcgggca cgggcaagac gaccctgacg cgcgtgctcg cccggcggct
    11821 ccaacgcgag gggtggacga tattcgaggc gagcgccgca gacctgatcg ccgggcagaa
    11881 gtacatcggc gagatcgagg ggcgcgcgca aacgttcgtg cgccagatcg gcggcaagcg
    11941 cgtgctctgg gtcgtgccgt cgtttcagga gctgctgtgg gcgggccggc accagtacag
    12001 cccgacgggg gtgctcgacc tcgtcatgcc gcagatcgag tcgggcgcca tcaaggtggt
    12061 cggcgagctg acgagcgtgg cctacgagaa tttggtgcgc cagcgcccga agctgcgcgc
    12121 ggccatgcag accgtgaggg tcgcgcccat gcccgacgcc gagaccttcg agctgggccg
    12181 gcgctgggcc gagctgcacg cgccggctga gggcgacgac gaccacaacg cgcgcgtctc
    12241 gccggagacg ctgcgcgagg cgttccaact gacgaagcag tatctcaacg acaaggccgc
    12301 gcccggcaac ctcatcctcc tactcgactc gacgcatcgt cgtctcgtga ccgagcaggg
    12361 cgcgaaccgc ggacagattt ctttagacga cctcctcgtc acgctctcgc agttgacggg
    12421 gctgcccacg caaatcctcg acgaccgcgc cggtctcgac ctctcggagc tgcgcgcctt
    12481 cttcgagcgg cgcgtgatgg ggcagacgga ggccgtcgat tgcctcgtcg agcgcgtggc
    12541 gatggtcaag gcgggactga ccgacccgac gcgcccgcag ggcgtcttcc tcttcgtcgg
    12601 cccgacgggg acgggcaaga ccgagatcgc caagacgctc gccgaatttc tcttcggctc
    12661 gcccgagcgc ctgattcgcc tcgacatgag cgagtttcag acgtccgaaa ccctcgaccg
    12721 catcctcggc gagaccacgg acgcgggcga ctccaccgcc ctcgtcaact ccatccgcaa
    12781 gcagcccttc tccgtcatcc tcctcgacga gttcgagaag gcgcacccga acgtctggga
    12841 cttgttccta caggtcttcg acgacggccg cctcaccgac cgacgcggcg cgacggccga
    12901 cttccgacac tgtgtcctca tcatgacctc gaacctcggc gcgacgctcc cgacgggcgc
    12961 gagcattgga ttctcacacg agcaggcgca gttcacctcc ggcgcggtcg agcgcgccgt
    13021 gggccgctcc ttccgtcgcg agtttatcaa ccgcatcgac cgcgtcgtcg tcttccgccc
    13081 gctctcgctc tcggcgatgc gcgacatctt gcggaaggag ttgaacgacg tgctcggccg
    13141 gcgcggcttc cgcacgcggc agtgggcggt cgagtgggat gagtcggcca tcgacttcct
    13201 tttgcaaaag ggcttcacgg ccgacctcgg cgcgcggccc ctcaagcgtt ccgtcgagcg
    13261 ttacctgctc tcgccgctct ctctgaccat cgtcaaccac cagttccccg agggcgacca
    13321 gttcctactc gtgcgcgccg gcggcgacga ctctatcgaa gtcgagttcg ttgaccccga
    13381 cgcgcccgag tcgccgcccg ccgcggcttc cgtgattgat gaagaggaag ccgtcgaaga
    13441 gtctgccgag cggcgtctcg aatccatcgc gttggacgcg cgcggcacgg gcgctgaagt
    13501 gtctctgctc cagagcaagt acgacgagtt gcgcgcgcag gtagactcgg acgagtggca
    13561 gcagcgcaag caggacgcgc tgctccagac cgcccgcccc ggcttctggg attcgcccga
    13621 acgcttcggc gtgttaggtc ttgccgagta catggatcgc atcgaggccg gtctcgacac
    13681 ggccggctcg ctcttggcgc gtctgacggg cggacgccac cgcgcggatg agcggcgcgc
    13741 atatctaccc gacctcgtcg agcgcgtcgc gcagcagctc tatcttgtgg acgcggcctg
    13801 ctgcgggatg ggcgcgggcg cggcgcgcga cgccttcctg ctcgtcgcgg ccgtgcgcga
    13861 gacgggcgcg gatgcgccgg ccgccgaccg cttcgcacgc cgtgtcgcgc ggatgtaccg
    13921 gcgctgggcc gagcgtcggc ggatgcgttt cgaggtgctt gaagagtcgg gcggcgaggg
    13981 cacgggcggc gcgggcgctt accgcctgtc ggccgccgtc tccggctttg ccgcctactc
    14041 catccttcag gccgaggcgg gcctccacgt ccttgagacg ccgcagcggg agcgcagcac
    14101 gttcaaccgc gcgagcgcgc aggtgagaat cgtcgcgcag ccagaggagc cggccgggcg
    14161 cgcccccgac gcgctccggc gtcaggccga ggccgcgctc gaacaggacg ccagcgcaca
    14221 gagcgccgcc aacaccatcg tccggcgcta tcgagaagaa ccgtcgccgc tcgtgcgcga
    14281 ctccgtgcgc ggctggcgca cgggcaaact cgaacgcgtg ctcgacggcg acttcgacct
    14341 gttccgctga agacaaccag acgctcgtcg tcgtcgttcg cttttatctg cgcgcggaat
    14401 gtaagatcac ccgcgccggc gtcttgaagt acgtgagtgg agcgccgtcg ggacggtctt
    14461 tgccttttga gccagaagtg cgcgcggagt ttccgggatg aatcgtcaac gcctactgtc
    14521 gtggttgctg cgtctcgcgg ggacgttcga gatgctcgcg ttcatctccg tggtgatgcc
    14581 gcgcgcgtgg atggaggcgt cgcacgcgtg gctgggcctc ggcgagatgc cgggcggccc
    14641 cgtcctcatc tttatgatcc gacaggcgtc ctactcctac gggatgcacg gaatatctct
    14701 gtggattctg gccacggacg taaaacgctt ccgcccgctc gtcgtattca acgccgtcgc
    14761 gttcctgctg gccgcgcccg tgttcttcat catc
//
LOCUS       DASVES010000087         3546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_441274, whole genome shotgun sequence.
ACCESSION   DASVES010000087 DASVES010000000
VERSION     DASVES010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441274"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1701)
                     /locus_tag="VF240_03095"
     CDS             complement(178..1701)
                     /locus_tag="VF240_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7174260.1"
                     /translation="MAKHSNSIPFAAVSVDGDSKVPLYRQLYENLRQAILTGQLAPGT
                     RLPSTREVAAELHLSRNTVMNAFEQLLAEGYVEGHVGSGTFVSQALPDELLTARTGVR
                     RALRPRRGRRSVSRRGELLATTPVRACCPALGGVVPFRTGVPALDAFPFDVWQKINAR
                     HWRRPQRDLMGYGDPAGHRPLREAIAAYLGAARAVRCRPEQVIVVAGTQQAVDLVARV
                     LLDPGDAVWVEDYGYLAARGALRAAEARLVPVPVDEEGIDVAAGARLCADARLAYVSP
                     SHQYPLGVTLSLGRRLALLEWASRTGAWVVEDDYDSEFRYSGRPLAAMQGLDEEGRVI
                     YLGTFSKVLFPGLRLGYMVVPEDMVEAFTAARALVGWCSPVVDQSIVADFIGEGHFAR
                     HIRRMRALYAERQALVVAAARRELAGLLEVEPAAAGMHLTGWLPEGVDDAATSRACVA
                     AGVQTQPLSAFALERHYRRGGLLLGYAAYNEREIRDGVRKLALTLEGILRASRRKVS"
     gene            2035..3264
                     /locus_tag="VF240_03100"
     CDS             2035..3264
                     /locus_tag="VF240_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7174261.1"
                     /translation="MSAQKMERRTYSFARRVENMGVSAIREILKVTEQPEVISFAGGL
                     PAPELFPVEALAEANARVFAEEGAAAMQYSTTEGWRPLREWIARRLIGRGIASAEAGR
                     ILVTSGSQQGIDLAAKAFLDPGDGVVVENPCYLAALQVFNGYEAQLLAVGSDDEGMRV
                     DEVERAIAGAKQKPKLIYVVSQFHNPKGTTLALERRRQLIEVSRRHGVPILEDDPYGE
                     LRYSGAQLPPLAALDHDGLVIYLSTFSKTLSPGMRVGWVHASDEIIQALVRAKQAADL
                     HTNTIQQRAAATLLETFDFDGHIEHIRQVYGERCAVMLECVGKYFPEGVRPTRPEGGL
                     FLWVELPERVRAEELFEEALRERVAFVPGAPFFAAKAQHNFMRLNFSNSPPEKIEEGI
                     RRLGQVLTQMSEQYRER"
BASE COUNT          621 a         1178 c         1185 g          562 t
ORIGIN      
        1 ttagcattaa ctctggaagg gattttgcgc gcctcccgca ggaaggtgag ttgagcgtct
       61 ttcacgctca ctcaccgcag aggcgcggag gacgcggagg gagcgccgca gggttgaatc
      121 tctccgcgcc ccctctgcgt cctctgcgcc tctgcggtga gtgagcgtga aagacactca
      181 actcaccttc ctgcgggagg cgcgcaaaat cccttccaga gttaatgcta atttccgaac
      241 gccgtcgcgt atctcacgct cgttgtaggc cgcgtagccg aggagcagtc cgccgcgccg
      301 gtagtgtcgc tccagcgcga aggccgagag cggctgcgtc tgcacgccgg ccgcgacgca
      361 ggcgcgcgag gtcgccgcgt cgtccacgcc ttcggggagc cagcccgtca ggtgcatccc
      421 ggcggccgcc ggctcgacct ccagcaaccc cgccagctct cgccgcgccg ccgcgacgac
      481 taatgcttgc cgctccgcgt agagcgcgcg catgcgccgg atgtggcgcg cgaagtggcc
      541 ctcgccgatg aagtcggcga cgattgactg atcgacgacg ggcgagcacc agccgacgag
      601 cgcgcgcgcc gccgtgaacg cctccaccat gtcttcgggc acgaccatgt agccgaggcg
      661 caagccgggg aagagcacct tgctgaaggt gccgaggtag atgacgcgcc cctcctcgtc
      721 aagcccctgc atcgccgcca gcggccggcc cgagtagcgg aactcgctgt cgtaatcatc
      781 ctcgacgacc cacgcgcccg tgcggctcgc ccactccaga agggcgaggc gccgccccag
      841 actcagggtc acgccgagcg ggtactggtg cgaaggcgag acgtaggcga gccgcgcgtc
      901 cgcacaaagc cgcgcgccgg ccgccacgtc gatgccttcc tcgtccacgg ggacggggac
      961 gagccgcgcc tcggccgccc tgagcgcgcc gcgcgcggcg aggtagccgt agtcttcgac
     1021 ccacacggcg tcgccggggt cgagcaggac gcgcgccacg agatcgaccg cctgctgcgt
     1081 gccggcgacg acgatcacct gctcgggccg acagcgcacg gcgcgcgccg cgccgagata
     1141 ggcggcgatg gcctcgcgca gcggacggtg gccggccggg tcgccgtagc ccatgaggtc
     1201 tctctgcggg cgtcgccagt gacgcgcgtt gatcttctgc cacacgtcga aggggaaggc
     1261 gtcgagcgcg ggcacgcctg tgcggaaggg gacgacgccg ccgagggccg ggcagcaggc
     1321 gcgcacgggc gtcgtcgcca gcagctctcc gcgccgcgac acgctccgcc gcccgcggcg
     1381 cggacgcagg gcgcgccgca cgcccgtgcg cgccgtgagc agttcgtcgg gcagcgcttg
     1441 cgagacgaag gtgccggagc cgacgtgtcc ttcgacgtag ccttcggcga ggagttgctc
     1501 gaaggcgttc atcacggtgt tgcgcgacag atgcagctcc gcggcgacct cgcgcgtgga
     1561 gggcaggcgg gtgcccggcg cgagctgtcc ggtgagaatc gcctgacgca gattctcata
     1621 gagctgccgg tagagcggga ctttcgagtc gccgtccacg gagacggcgg cgaaggggat
     1681 gctgttggaa tgtttcgcca tcacggtttc ctctgaacgt aatgctagaa caaattggca
     1741 ccctgcgtct aagaaaaaat ggcgcttgtg cgggggccaa ttccgctgct agtgtgcaaa
     1801 cgtccaggtg tgtgtatttg ttcgacggcg gggcggctgg cgaagaatcg ttaacatgga
     1861 tggacaggaa gagaacaagt gatgggtgat aagtgatgag tgatgagtga tgagacaaaa
     1921 accgtctgct ctttctcatc actcatcact catcactcat cacttcttgc atcctgttca
     1981 tccatgtgaa attgtttttg tagaagtgga taaaccagaa tcggacatga ctacatgagc
     2041 gcacagaaaa tggaacgccg cacatattca ttcgcccgac gcgtggagaa catgggcgtc
     2101 tcggccatcc gcgagattct gaaggtcacg gagcagcccg aggtgatctc gttcgcgggc
     2161 ggactgccgg cgccggagtt gtttcccgtc gaggcgttgg ctgaggcgaa tgcgcgcgtc
     2221 tttgccgaag aaggcgcggc cgcgatgcag tacagcacga ccgagggctg gcggcccctt
     2281 cgcgagtgga tcgcgcgacg actcatcggg cgcgggatcg cgtcggccga ggcgggccgc
     2341 atcctcgtca ccagcggctc gcagcagggc atcgacctcg cggcgaaggc gttcctcgac
     2401 cccggcgacg gggtggtcgt cgagaacccc tgctacctcg ccgcgcttca ggtcttcaac
     2461 ggctacgagg cgcaactcct cgccgtcggc agcgacgacg aagggatgcg tgtggacgaa
     2521 gtcgagcgcg ccatcgccgg cgcgaagcaa aagccgaaac tcatctacgt cgtctcgcag
     2581 ttccacaatc cgaaggggac gacccttgcg ctcgaacggc ggcggcaact catcgaggtc
     2641 tcgcgccgcc acggcgtgcc cattctcgaa gacgaccctt acggcgagct gcgctactcg
     2701 ggcgcgcaac ttcccccact cgccgccctc gaccacgacg gcctcgtcat ctatctcagc
     2761 accttctcca agacgctcag ccccggcatg cgcgtcggat gggtacacgc ctccgacgag
     2821 attatccagg ccctcgtgcg cgccaagcag gccgcagacc tccacaccaa caccatccag
     2881 cagcgcgccg ccgccacgct ccttgagacc ttcgacttcg acggccacat cgagcacatc
     2941 cgtcaggtct acggcgagcg ttgcgcggtg atgctcgaat gcgtcgggaa atatttcccc
     3001 gagggcgtgc gcccgacgcg gcccgagggc gggctgtttc tgtgggtcga gttgccggag
     3061 cgcgtccgcg ccgaggagtt gttcgaggag gcgctacgcg agcgcgtggc cttcgtcccc
     3121 ggcgcgccct tcttcgccgc caaagcgcag cacaacttca tgcgcctcaa cttctcgaac
     3181 agcccgccgg aaaagatcga ggagggcatc cgccgtctcg gccaggtttt gacgcaaatg
     3241 tcggaacagt accgcgagcg gtagcgagcg ggtctgactc acacgaggcg ggtctctcgt
     3301 cgcagtcgca cccgcgcggc taccgctcgc gatactgtgc ccgccgaccg ggtgggactt
     3361 tggaatcagg agcatcactg atgagtgagc tgaagcagac cgatagaacc gaactcggca
     3421 ggcacccgga gcggggcgtg ttcgggcgcg agcgcgtcta cgagattctc gacgagggac
     3481 tggtgtgcca cgtcggcttc gccgtcgggg gcaaacccta cgtcatcccg acgggctacg
     3541 tccgcc
//
LOCUS       DASVES010000088         6130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_627244, whole genome shotgun sequence.
ACCESSION   DASVES010000088 DASVES010000000
VERSION     DASVES010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627244"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..361
                     /locus_tag="VF240_03105"
     tRNA            289..361
                     /locus_tag="VF240_03105"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ala,seq:ggc)
     gene            374..445
                     /locus_tag="VF240_03110"
     tRNA            374..445
                     /locus_tag="VF240_03110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:406..408,aa:Gly,seq:gcc)
     gene            547..619
                     /locus_tag="VF240_03115"
     tRNA            547..619
                     /locus_tag="VF240_03115"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Val,seq:gac)
     gene            630..711
                     /locus_tag="VF240_03120"
     tRNA            630..711
                     /locus_tag="VF240_03120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:664..666,aa:Leu,seq:gag)
     gene            848..919
                     /locus_tag="VF240_03125"
     tRNA            848..919
                     /locus_tag="VF240_03125"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:881..883,aa:Glu,seq:ctc)
     gene            964..1040
                     /locus_tag="VF240_03130"
     tRNA            964..1040
                     /locus_tag="VF240_03130"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:998..1000,aa:Asp,seq:gtc)
     gene            1166..1238
                     /locus_tag="VF240_03135"
     tRNA            1166..1238
                     /locus_tag="VF240_03135"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1199..1201,aa:Lys,seq:ctt)
     gene            1310..1386
                     /locus_tag="VF240_03140"
     tRNA            1310..1386
                     /locus_tag="VF240_03140"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Ala,seq:cgc)
     gene            1478..1550
                     /locus_tag="VF240_03145"
     tRNA            1478..1550
                     /locus_tag="VF240_03145"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1511..1513,aa:Phe,seq:gaa)
     gene            complement(2036..2473)
                     /locus_tag="VF240_03150"
     CDS             complement(2036..2473)
                     /locus_tag="VF240_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174262.1"
                     /translation="MMNSKSRGFLIRVFMLSLVAFLTLPASAFAQRNQRRREARQERR
                     EERREDRQERREERRGRNWERYGNYGGSANLRQTALNAGFNNGIEEGRKDRQRNERYD
                     FRDEGDYQKATEDYSSKLGNKELYKRYFREGFENGYEAGYNGY"
     gene            3179..4048
                     /locus_tag="VF240_03155"
     CDS             3179..4048
                     /locus_tag="VF240_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX7174263.1"
                     /translation="MLTSLFPGRKPVIGMIHVGALPGTPASTQGVSEIAAQAAREARV
                     YRHVGVDGLGIENMHDVPYLRAGALGPEVVAAMAVVGRAVREEAGLPTGIQILAGANV
                     AAMAVAHAAGLDFVRAEGYAFAHIADEGWIDSGAAELLRYRRHIGAERVQVWADVKKK
                     HSSHAVTSDVSLAATAEAVEFMRADAVVITGAATGEPPRADDVREARRACRLPVVLGS
                     GVDARNIADFFPDADAFIIGSYFKVDGHWANPVDARRIEKLMEIVNRLREGAGGGGAS
                     TADTLRARDGEHG"
     gene            complement(4144..5316)
                     /locus_tag="VF240_03160"
     CDS             complement(4144..5316)
                     /locus_tag="VF240_03160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7174264.1"
                     /translation="MTERADVVIIGGGVVGASIAYHLAEAGCRDVLVVERGQELGEGS
                     TGRATGGVRAQFATAVNIRMSLYSIDFFSRFSEATGRDCGYRPIGYLFLAGERQLAQL
                     ESNRERQRAVGLTCVEILSTDDIAARLPLLRLDDIAGGSFCPIDGLIDPLAVMRGLLK
                     RARERGVTLWTDTRVTGIDLDGGAVVSVDTSRGRVATRAVVNAAGPWAAEVARLAGVE
                     IPVTPLRRQLVGAQSFDRLPERMPMVIDLSDGFHFRRVEHGAQAPGVLLAWPDPEETP
                     GFNTDFDDDFTGKILERAARRVPAFAGLKVDRRLCRAGLYEMTPDHHAIIGRAPGVAG
                     FYLANGFSGHGVMHSPATGRAVSDLILHGESRAIDIHALRAERFAEGDLIEESVLL"
     gene            5470..5871
                     /locus_tag="VF240_03165"
     CDS             5470..5871
                     /locus_tag="VF240_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174265.1"
                     /translation="MKRILLVAAVVLSSAAGFAIRSLSLKASDAPAAEASAPAQDADE
                     REWWEYAAVTRAQFASSPRGGQYWIVYFGGESGVKPDTVEAGVGGNAQGKAIALLGSR
                     GWEMVGEGTLDTGRRERDEPPRALLFKRRRR"
BASE COUNT         1079 a         1847 c         2038 g         1166 t
ORIGIN      
        1 actctcaccg cccacacctt tctgtcgtcg atacaacgga gtcgaaagtg ccggaactgg
       61 ggcgcaaagg ggcatccggc gccggggtcg tcgcatcaaa aggtgcttca atccggcgcg
      121 tccgaacgag cgcacgtcgc cggggcgaaa aacgcgcggg gcgaaaacct caccgcaaga
      181 cccggaaata tcacccggcg agttgcatcg aaggcggtgg tggcttatcc ttcgcacttt
      241 cgccgtgacg ggcggacgca gttgtttgat aacacgaacg cttttttcgg ggctgtagct
      301 cagttggcag agcgcttgaa tggcattcaa gaggtcagga gttcgattct cctcagctcc
      361 atccgcgtga ggcgcgggag taactcagtg gtagagtgcg accttgccaa ggtcgaagtc
      421 gcgggttcaa atcccgtctc ccgctttcgg gcagggatga aggcggaggg atgagggatg
      481 aagaagaatt gttttattga gtcaatccta ttcatccttc atccctcatc cctcatcctt
      541 tgcttcgggc gcttagctca gttggtagag tactaccttg acacggtagg ggtcagaggt
      601 tcgagtcctc tagcgcccat tggggaaaag ccgaagtggc ggaactggta gacgcgcacg
      661 tttgaggggc gtgtgaggta actcgtgggg gttcgagtcc ccccttcggc atcatggcgg
      721 cgcacgccgt ttgacaacgc gtcgcttttg cggcgaccat ggccacagac ttttccaaag
      781 tgctcaaccg tggtcaggag cgcgcggccg gcgtcccctt ccgaggatgc cgctggaaac
      841 tttgtcaggc ccgttcgtct aggggttagg acgtagccct ctcaaggcta aaacacgagt
      901 tcaattctcg tacgggctat tgaaattgtg cggcttggtc gcacgtcagg tttgaaaggt
      961 tatggagcgg tagttcagtt ggttagaacg ccggcctgtc acgtcggagg tcgcgggttc
     1021 gagtcccgtc cgctccgcca taatcaaaag cccctgtggc tcagtttgtg ttgcagacga
     1081 ctctcacatc tgcattgagc cgggggccaa gtgtgccgac tgccaccaga gaaggttcgc
     1141 tccggggtca ggttggcgca agttcggatc ggtagctcag ttggtagagc aacggactct
     1201 taatccgtgg gtcgtgggtt cgagccccac ccgatccatt gagttgaagg taggaatgcg
     1261 ctcaaggtcc gcacgggggc cgcacctaca ctttacactt tcaatcctcg gggcagtagc
     1321 tcagtttggc agagcgccgc gttcgcaatg cggaggccag gggttcgagc cccctctgct
     1381 ccaccaagac taagggatga gggcagaagg gatgagggat gaagtaaggc gagttcttat
     1441 tcatccctca cccttctgcc ctcatccctg acgttccggc caggtagctc agttggtaga
     1501 gcacgcgact gaaaatcgcg gtgtcggcgg ttcaattccg tccctggcca tcaattgatc
     1561 tacacagtta cggggagtcg catggcttcc cgtttttcgt ttatgtgcca actcaaaagg
     1621 aagattcttt tgtagtatgg cactcgaccg cgagtcagcg tagctctcca ctgaagcttg
     1681 tccgtagcgc agttgaatct gcggcgggga atgtcagacg taaggcttct aacttttccg
     1741 tagcgggagg agtgtaatga tgcctaagtg gttgttattt ggtctcgctg ctataggtaa
     1801 cttcatggta gccgtaataa cttaccgcgg tggaagactc atcatcccga tgatcctcgc
     1861 cgtcgcgggg ttgtgcttcg ttatcgccgc agccggggcc gcgagagagg cggggggcgg
     1921 tcgcgcctga agcggcgcgg ctgatgggtg aatatgaggg ggcgaggtgg gcgtcgaaga
     1981 aagaggggcg ggtcatgaaa cccccgcccc ctgccctaaa cccatgagtt tggttttagt
     2041 acccgttgta gccggcctcg tagccgttct cgaacccctc gcggaaatac cgcttgtaga
     2101 gttccttgtt gccgagcttg gagctgtagt cctccgtcgc cttctggtag tcgccctcgt
     2161 cgcggaagtc gtagcgctcg ttgcgctggc gatccttgcg cccctcctcg atcccgttat
     2221 tgaagccggc gttgagggcc gtctggcgaa gattggccga gccgccgtag ttgccgtaac
     2281 gctcccagtt gcgaccgcgg cgctcctcac gccgctcttg ccgatcctcg cgtctctcct
     2341 cgcgccgttc ctgtctggct tcacgccgcc gttgattgcg ctgggcgaaa gccgacgcgg
     2401 gaagcgtcag gaacgcgacc agcgacagca tgaacaccct gattaagaat cctcgacttt
     2461 tgctattcat catctcccct cacttccaac actcttgttg atgtccggtt aagatcccgg
     2521 ttaacgcact ctgacgcggc tcattagtct cgtctgcgtt cgtccgttcg gcaagtatct
     2581 cacttgataa gtctcgcggt aaacctgcct cccgcggcgc acgaggcgcg tttgcgtcac
     2641 gacacggacg cggccgttat tgcgccccct tctgattcga tgtggccact gcggctcaac
     2701 cgcgttagcc gcgatggtat tcgccgcgga gaccgctgac tcagcggcct tcgccttcgc
     2761 gggcgaagcg acgaaaccga ttgcagccag tgacagcgta agtcctaaca gtttcttcat
     2821 gtctatacct ctctaaagaa ttaagccgag tgcaaccacg gcgcgttcac ggcgcccagt
     2881 gggagaactc ggcctaataa ctcagacacc actaaagagc agatagttac gctcgggtct
     2941 tggcgaggat gtataaccca aattatgtgg catcgaaaac tcatgcttgt cacgcaacta
     3001 acacgccgcc tcagacgatg gcccgtgtcc gagcgcgcat cagccggaac gtcgttggga
     3061 acttgccgct atctgccttg agtgcgtggc ggatgacgcc ggggggtgga aagcgtcggc
     3121 ccgcgcacgg cgggattcag ttgacacgga cgccggcgcg ggggtttatt gggtgcccat
     3181 gctcacttcc ctgttccccg gacgcaagcc ggtcatcggc atgattcacg tcggcgcgct
     3241 gcccggcacg cccgcctcca cgcagggcgt ctccgagatc gccgcgcagg cggcgcgcga
     3301 ggcgcgtgtc taccgccacg tcggcgtgga cggcctcggt atcgagaaca tgcacgacgt
     3361 gccctacctg cgggcgggcg cgttggggcc ggaggtggtc gcggcgatgg cggtcgtcgg
     3421 gcgcgcggtg cgcgaagagg cggggttgcc caccggcatc cagattctcg ccggggcaaa
     3481 tgtggccgcg atggccgtgg cgcacgccgc cggtctcgac ttcgtccgcg ccgaagggta
     3541 cgccttcgcg cacatcgccg acgagggctg gatagattcg ggggcggcgg aactcctgcg
     3601 ctaccgccgc cacatcggcg ccgagcgcgt gcaggtgtgg gccgacgtga agaagaaaca
     3661 ctcgtcgcac gccgtcacga gcgacgttag tctcgcggcg acggccgagg ccgtggagtt
     3721 catgcgcgcc gacgccgtcg tcatcaccgg cgccgcgacg ggcgaaccgc cccgcgccga
     3781 cgacgtgcgc gaggcccgtc gcgcctgccg cctccccgtc gtcctcggct ccggcgtgga
     3841 cgcccggaac atcgccgact tcttccccga cgccgacgcc ttcatcatcg gctcctactt
     3901 caaggttgac ggccactggg ccaaccccgt cgatgcgcgg cgcatagaga agttgatgga
     3961 gatcgtcaac cggctccgcg agggcgccgg cggtggaggc gcgtccacgg ccgacacttt
     4021 gagggcgcga gacggggaac acggatgaga cggatgagca cggattaaca cggattgaat
     4081 ccgtgacttt ccgtctcatc cgtcctatcc gtgttctatt ctctccggcc gccgacgcga
     4141 ctttcagagc aacaccgact cctcgattaa atccccctcg gcgaatctct cggcgcgcaa
     4201 ggcgtggatg tctatggcgc gcgactcccc gtgcaggatg aggtcggaga cggcgcggcc
     4261 ggtggcgggc gagtgcatga cgccgtggcc gctgaagccg ttggcgagat agaagcccgc
     4321 gacgccgggc gcgcggccga tgatggcgtg gtggtcgggc gtcatctcgt agaggccggc
     4381 gcggcacaag cggcggtcaa ccttgagacc ggcgaacgcg gggacgcggc gcgcggcgcg
     4441 ttcgaggatt ttgccggtga agtcgtcgtc gaagtcagtg ttgaagccgg gcgtctcctc
     4501 ggggtcgggc caggcgagga ggacgccggg cgcttgtgct ccatgctcca cacggcggaa
     4561 gtggaagccg tcggagagat cgatgaccat cggcatccgt tcgggaagtc ggtcgaagga
     4621 ttgggcgccg acgagttgcc ggcgtagcgg cgtgacgggg atttcgaccc cggcgaggcg
     4681 ggccacctcc gcggcccacg gcccggcggc gttgacgacc gcgcgtgtcg cgacgcggcc
     4741 ccgcgacgtg tcaacgctga cgaccgcgcc cccgtcaagg tcgattcccg tcacacgcgt
     4801 gtccgtccac agtgtgacgc cgcgctcgcg cgcgcgtttc agaaggccgc gcatcacggc
     4861 cagagggtcg atgaggccgt cgatgggaca gaaggagccg ccggcgatgt cgtcgagccg
     4921 gagcagcggc agacgcgcgg cgatgtcgtc ggtcgaaaga atctcgacgc aggtcaggcc
     4981 gacggccctc tgccgctccc ggttcgattc gagctgcgcg agttgtcgtt cgccggcgag
     5041 gaagaggtag ccgatgggtc ggtagccgca gtcgcgcccc gtggcctcgg agaagcgtga
     5101 gaagaaatca atcgagtaga gcgacatgcg gatgttgacg gccgtggcga actgcgcgcg
     5161 cacgccgccc gtcgcgcggc ccgtcgagcc ttcacccaac tcttgcccgc gctcgacgac
     5221 gagcacgtcg cggcagccgg cctccgccag atggtaggcg atgctcgcgc cgacgactcc
     5281 gccgccgatg atgaccacgt cggccctttc cgtcatacgt gtcatcatac ccatacgcgc
     5341 ggtgcgccgc gaaggccgca gcgcgcgtcg cttctttcct gaaaccatct ttgcggcact
     5401 tccagccggt gcgcgcgtgt ttgcgttagt atcgtcggtc gagagactta tgaaggagga
     5461 tacccatcga tgaaaagaat tcttttagtc gcggcggtcg tgctttcgtc ggccgcgggt
     5521 ttcgccatcc gctcgctgtc gctcaaggcg tcagacgccc cggcggccga ggcgtcggcg
     5581 ccagcgcagg acgccgatga gagagagtgg tgggagtacg ccgcggtcac gagggcgcag
     5641 ttcgcatcca gcccgagggg cggacagtac tggatcgtct acttcggcgg cgagagcggc
     5701 gtgaaacccg acacggtcga agcgggggtg ggcggcaacg cgcaggggaa ggccatcgcg
     5761 ttgctcggat cgcgtggctg ggagatggtc ggcgagggca cgctcgacac cggccggcgc
     5821 gagcgcgacg agccgccgcg cgccctgctc ttcaaacgcc gcaggcggta gacgtggaca
     5881 cgtttagaat gactctcact tgagtgaagg cgaagaagat tcaccgcgga ggcgccgcag
     5941 gacgcggagg gggcgcggag ttgtccacgt ttaaagtgga agctgtaagc gcgccacgca
     6001 gccgcggatg ccgatgcggt tctggagggt gagggtgccg ccgtgggcct cggcgatctg
     6061 gcggctgagg acgaggccga tccccgaccc ttcgggcttc gtggtgaaga agggcacgaa
     6121 gaggttgctc
//
LOCUS       DASVES010000089         9975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_196722, whole genome shotgun sequence.
ACCESSION   DASVES010000089 DASVES010000000
VERSION     DASVES010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196722"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..865)
                     /locus_tag="VF240_03170"
     CDS             complement(92..865)
                     /locus_tag="VF240_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174266.1"
                     /translation="MTMDVRAYNRDAWDKEADDGGQWSSPVAAEVIAAARRGEWKLRL
                     TETKYAPREWFPDLHGLEVLCLASGGGQQAPTLAAAGARVTVLDNSPRQLAQDRLVAE
                     RDSLDLTTVEGDMADLSAFADESFGLVYHPVSNLFVPDVRPVWREAFRVLRPGGLLLA
                     GFLNPVLYLFDDRLGEGDGLKLRHKVPYSDLTSLTEEQRQRLVAANLAFEFGHTLADQ
                     IGGQTDAGFHITGFYEDIHPGLEIAKYIPTYGATRALKP"
     gene            complement(996..1559)
                     /locus_tag="VF240_03175"
     CDS             complement(996..1559)
                     /locus_tag="VF240_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7174267.1"
                     /translation="MNRSFTRVLKLALALGCALVIFALTLSGRAAAGLSASAQATPTP
                     TPAAPTQTSAPQASASFDQAKALSDLRARIAGQETKPAAEVFKNIQILKTVPAGRLLA
                     VMEVGYSRSLGVDCTHCHLPGEWEKDDKATKGVAREMITMMRAINEEHLKKIKNLRSE
                     TPVVNCTTCHRGQTKPALNLPDARPRQ"
     gene            complement(1570..2043)
                     /locus_tag="VF240_03180"
     CDS             complement(1570..2043)
                     /locus_tag="VF240_03180"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX7174268.1"
                     /translation="MPTKKSFRVKLESCTGSQAARFTIPFDAEKVFGTRARVPVRGTL
                     NGHPFRSSIFPTGEGSHYMVVNRETREGAKVKAGDTVSFVMERDEETRVIVAPPELAR
                     ALSANKNAKAAWDKLSYTRQKEHARAVEEARRPETRARRVEKVIAELSAGRKSSR"
     gene            complement(2156..3082)
                     /locus_tag="VF240_03185"
     CDS             complement(2156..3082)
                     /locus_tag="VF240_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX7174269.1"
                     /translation="MLVEIHAKTDIGRIRSGNEDNFLVLDLGSSQAWTGTQKSEEDAG
                     LHRFEIGPKGAVLAVSDGMGGALAGDVASRMAVETVRDEVLKSASEDGEAASESPLVN
                     CLRYATEFANLRIHRSSQEEARHSGMGATFTGVAVTPGGVDFVQVGDSRAYIIRGREI
                     RLATKDQSLVQQLVDVGQISEQEAETHMFRNVILQALGAQAELTPVTGRVRLRRDDII
                     LVCSDGLSGKLRAEDMLDIVASSGGDLDAACARLVDEANARGGEDNITVILARFSGDD
                     LESPDSHKITVELPDLDDDSTFSGSEDETKNP"
     gene            complement(3161..4303)
                     /locus_tag="VF240_03190"
     CDS             complement(3161..4303)
                     /locus_tag="VF240_03190"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX7174270.1"
                     /translation="MGFSTTAIHVGNEPDPSTGAVTVPIYQTSTYAQDGLGRNKGYEY
                     ARTQNPTRTAMEQNVAALEGARFGYAFASGMAAIDATLRLIKGGEHVIVSDNTYGGTF
                     RLFDKILKNYGIEFTFVDTSDASNVEAAIRDNTRMVFVETPTNPVMIVSDLQAVSDVA
                     HRAGARVVCDNTFMSPYLQRPMDFGVDIVVHSTTKYLNGHSDGVGGCVVLNDERDAEW
                     IKFVQNGVGAIISPFDAWLVLRGTKTLALRMEQHDRSGRAVAAFLEEHPKVQKIYYPG
                     SVSHRQHELAKRQQKGFGGMIAFDVGSLDAARTVLESVQLCTLAESLGGVETLISHPL
                     TMTHASVLPETRERLGITAGLVRVSVGLEDTEDIIADLDQALGKLP"
     gene            4624..5466
                     /gene="kdsB"
                     /locus_tag="VF240_03195"
     CDS             4624..5466
                     /gene="kdsB"
                     /locus_tag="VF240_03195"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX7174271.1"
                     /translation="MDNTESRRTSPTVVAIIPARYASTRLPGKPLLEIAGKPMILHVV
                     ERALAARCVARVLVATDDARVAEAVRGAGREAVLTRADHRSGSDRLAEAVESVDAEIV
                     VNLQGDEPFVSPLTVERAVAALIADETAQVSTTCEEIESADDVLNPGVVKVVTDERGY
                     ALYFSRAPVPFPRDAALHHGSLQAALANDPTLLKGFRKHTGLYVYRRAFLLEYARWPQ
                     SPLERAESLEQLRILERGAKIKVVEAASPSIGVDTPEDLARVRAMIDAQSSQVEPRLK
                     WPGF"
     gene            5496..5831
                     /locus_tag="VF240_03200"
     CDS             5496..5831
                     /locus_tag="VF240_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174272.1"
                     /translation="MGDWIGLGAIILVALGAFFGLARLGTPARPMTQEEFEQKVQEGR
                     GWMSAGAFAGMQALQKLMNPKAAEAVEVQKDLQAGYYDDQQEVGDGDEPVDPAAEEEK
                     RTTEEGTDA"
     gene            5824..7527
                     /locus_tag="VF240_03205"
     CDS             5824..7527
                     /locus_tag="VF240_03205"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7174273.1"
                     /translation="MPKYIFVTGGVVSSLGKGLAASSIGSLLEARGLRVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTNARLTQGNNWTTGRIYLSVIEKE
                     RRGDYLGKTIQVIPHITDEIKRAVRAVAEKDPVDVVIVEIGGTVGDIESLPFLEAIRQ
                     MGNEERQDGGKGAVFVHLTLVPYIAAAGELKTKPTQHSVKELREIGIAVDILLCRSDR
                     ALSRDLCSKIALFCNVRESAVITAQDVETIYEVPLAFHEQGLDELIVTSLGLEERAAQ
                     SDLTRWRQLVSTIKEPSAGSVAIAIVGKYVELEDSYKSLREALTHGGVANNVRVDVRW
                     VESEGMMDEDYEERLRDYDAILVPGGFGKRGVAGMIRAITYARKSKTPYFGICLGMQT
                     ATIEYARSVCGLLGADSTEFDAETPYPVIFKLRDLVGVEEMGGTMRLGEYPCNLAPGS
                     LARVVYGGSESIGERHRHRYEFNPEFREQLERAGLVFSGESPDGRFVEMVELPREVHP
                     WFLGCQFHPEYKSKPLAAHPLFASFVRAAYENRMRDEATIEDAAEAPAHEHAHVVAGD
                     D"
     gene            7639..8211
                     /locus_tag="VF240_03210"
     CDS             7639..8211
                     /locus_tag="VF240_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174274.1"
                     /translation="MNIINPEIETERLLLRAFTPADFEAFAAMRADAEVMRFIGMPVG
                     PHTREQARAWQERNTRRWQDNGFGMWAVVEKATGELAGWCGLSRLDETQEVEVGYGLA
                     RRAWGRGLATEAARASLRYGFERMGLTRLVAVVNPANHASRHVIEKLGLRYVKDAYHY
                     GADLNYFEINRAEFKPTATPYVLRETPANG"
     gene            8233..8565
                     /locus_tag="VF240_03215"
     CDS             8233..8565
                     /locus_tag="VF240_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174275.1"
                     /translation="MRIIVFILTALVQSAVAVVIFLMLLLGLNGFSESDATPSLIFFI
                     ALSVVCALGLGGAAAYTAKRIAEKKSLGGVAASAIAVLCFSVLGGVILVGGFFAALVI
                     ATIMHEWK"
     gene            8594..>9975
                     /locus_tag="VF240_03220"
     CDS             8594..>9975
                     /locus_tag="VF240_03220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7174276.1"
                     /translation="MLQCPTCGRQFAADVTNCPDDGALLRADATIAIDAAAADPLVGR
                     VLDEKYRLDARLGEGGMGTVYRATHLLIERPVAVKVLNSRLVTDESARERFRREARAA
                     GRLQHTNAVAVTDFGETKDGLVYLVMELLEGRPLREVVAREAPLDPARAVSVMLQVSA
                     AVAAAHEAGVIHRDLKPGNIFVVQRPSAPHIVKVLDFGIAKIAEDADGNPADTLTGTG
                     VMIGTPRYMSPEQCDGAELTPAADVYSLGVILYEMLAGQTPFSGATPLALALKHSSET
                     PPRPSEFAPGIQPALEAVVLHALEKSPDERPADAGEFRRELFEVAERLGLEHAEGFSS
                     PTIETLRDAGTETPSGRLVIDIERLRRQRATNTLPAAHDTAEEEAARTAGRRDLPPPD
                     PAHAGAMPNRPRPAVARRERVGQNRFRVRVPTRRGEQSWASYLRQPNVAWLALAAVLF
                     VGGGFLLYRA"
BASE COUNT         1697 a         3180 c         3315 g         1783 t
ORIGIN      
        1 gagcggtagc gagcgggttc ccgcacccga gcacacccgc tcgctaccgc tcgcggtact
       61 gtccgcgatc atgtcgggcg tgatctcagg attaaggttt gagagcgcgc gtggcgccgt
      121 aggttgggat gtacttcgcg atttcgaggc cggggtggat gtcctcgtaa aacccggtga
      181 tgtgaaagcc cgcgtccgtc tgtccgccga tttgatcggc cagcgtgtgg ccgaactcga
      241 aagcgaggtt ggcggcgacg agtctttgcc tctgttcttc ggtcaggctc gtgaggtcgg
      301 agtagggaac cttgtgacgg agcttcaggc cgtcaccctc cccgagccgg tcgtcgaaga
      361 gatagagcac tggattcagg aagcccgcga ggagcagccc gccggggcgg agcacgcgga
      421 acgcctcgcg ccagacggga cgcacgtcag ggacgaagag gttggagacg gggtggtaga
      481 cgaggccgaa gctctcgtcg gcgaacgccg acaggtcggc catgtctcct tcgacggtgg
      541 tgaggtcgag agaatcgcgc tcggcgacaa ggcggtcttg cgcgagctgg cgcggagagt
      601 tgtcgaggac ggtcacgcgc gcgccggccg cggcgagggt tggggcctgc tggccgccgc
      661 ccgaggcgag gcacaagact tcgaggccgt gcaagtcagg gaaccactcg cgcggggcgt
      721 acttcgtctc cgtcaggcgc agtttccact cgccgcgacg ggccgccgca atcacctccg
      781 cggcgacggg cgacgaccat tggccgccgt cgtcggcctc cttatcccaa gcgtctcggt
      841 tgtaggcgcg aacgtccata gtcatcaatg gtaagtcgtc aatcgtgaat cgtaaatcgt
      901 gattgacgct ctccccgctt gagagaaaca tcggaagccg gacgatgctc aacttctatt
      961 cacgatttac gatttacgat tcacgactta cggtcttact gtctcggccg cgcgtcgggc
     1021 aggttgagcg cgggcttggt ctggccgcgg tgacaggtcg tgcagttgac gaccggcgtc
     1081 tcgctcctca ggttcttgat cttcttcagg tgctcctcgt tgatggcgcg catcatcgta
     1141 atcatctcgc gcgcgacccc cttcgtcgcc ttgtcgtcct tctcccattc gccgggcagg
     1201 tgacagtgtg tgcaatcgac gccgagcgag cggctgtagc cgacctccat gaccgccagc
     1261 aggcggcccg cgggaaccgt cttgaggatt tgaatgttct taaagacttc ggccgcgggc
     1321 ttcgtctcct gcccggcgat gcgcgcgcgt aagtcggaga gggctttcgc ttgatcgaag
     1381 gacgccgacg cctgcggtgc ggaagtttgc gtgggcgctg ccggcgtcgg cgtgggcgtc
     1441 gcctgcgccg atgcgctcag accggcggcg gccctgcccg aaagtgtcag cgcgaagatg
     1501 actaaagcgc agccgagcgc cagtgcgagc ttgaggacgc gggtgaaact cctgttcatg
     1561 attatctcct taccggctag acttccggcc cgcggacaac tccgcgatga ccttctcaac
     1621 cctgcgcgcg cgcgtctcgg gccgccgggc ctcctcgacg gcgcgcgcgt gctccttctg
     1681 gcgcgtgtag ctgagcttgt cccacgcggc cttcgcgttt ttgttcgcgc tgagagcgcg
     1741 ggcgagttcg ggcggcgcga cgatgacgcg cgtctcctcg tcgcgttcca tcacgaagct
     1801 gaccgtgtcg ccggccttca ccttcgcccc ctcgcgagtc tcgcggttga cgaccatgta
     1861 gtgtgagcct tcgcccgtgg gaaagatcga gctgcggaag ggatggccgt tgagtgtgcc
     1921 gcgcacgggg acgcgggcgc gcgtgccgaa aaccttttcg gcgtcgaagg gaatggtgaa
     1981 ccgcgccgcc tgcgagcctg tgcaactctc cagcttcacg cggaaactct tcttcgtagg
     2041 catgatttcc cccacgcggg aacaacttcc actttaggtt ttggaaccga cgggcatgcg
     2101 cgagtcgaac agttttcgat acggcgaggc ggcgggcgag gggcgggtcg gcgttctaag
     2161 ggtttttggt ctcgtcctcg gagccgctga aggtgctgtc gtcatcgagg tcgggcagct
     2221 cgacagtgat cttatgggag tcaggggatt cgaggtcgtc gccggagaag cgcgcgagga
     2281 tgacggtgat gttgtcctcg ccgccgcggg cgttggcctc atcgacgagg cgggcgcacg
     2341 ccgcgtccaa gtcgccgccg gaggaggcga cgatgtcgag catgtcctcg gcgcggagct
     2401 tgccggagag gccgtcgctg caaaccagaa tgatgtcgtc gcggcggagg cggacgcgtc
     2461 ccgtcaccgg ggtgagttcg gcctgtgcgc cgagagcctg aaggatgacg ttgcggaaca
     2521 tgtgcgtctc ggcctcctgc tcgctgatct ggccgacatc gacaagctgt tggacgagcg
     2581 actggtcttt ggtggcgagg cggatttcgc gcccgcggat gatataggcg cggctgtcgc
     2641 cgacctgaac gaaatcgacg ccgccgggcg tgacggccac gcccgtgaag gtggccccca
     2701 tcccgctgtg gcgcgcctcc tcctgactgc tgcgatggat tctgaggttg gcgaactcgg
     2761 tggcgtaacg caggcagttg acgagcggcg attcagaggc ggcctcacca tcttcgctcg
     2821 cgcttttcaa cacctcgtcg cgcaccgtct cgacggccat ccggctggcc acgtcgccgg
     2881 cgagcgcccc gcccatcccg tcagagacgg ccagcaccgc gcccttcggg ccgatctcga
     2941 agcggtgcaa cccggcatcc tcctcactct tctgcgtgcc cgtccacgcc tgcgaggagc
     3001 cgaggtcgag cacgaggaaa ttgtcctcgt tgcccgagcg gatgcgcccg atgtcggtct
     3061 tggcgtgaat ctctactagc atagtggata agtgatgagt gatgagtgat aagtgataag
     3121 aaaaacagct tccttcttat cacttatcac tcatcactta tcacggcaat ttgcctagcg
     3181 cctgatcgag atcggcgatg atgtcttcgg tgtcttcgag gccgacggag acgcgcacga
     3241 ggcccgcggt gatgccgagg cgctcgcgcg tctcggggag cacggaggcg tgcgtcatcg
     3301 tcagcgggtg cgagatgagc gtctccacgc cgccgaggct ttcggcgagc gtgcagagtt
     3361 ggacggactc aagcacggtg cgcgccgcat cgagcgagcc gacatcgaag gcgatcatgc
     3421 cgccgaagcc cttctgctgt cgcttggcca gctcgtgctg gcgatgcgac acggagccgg
     3481 ggtagtaaat cttctgcacc ttcggatgct cttcgaggaa ggcggcgacg gcccggcccg
     3541 agcggtcgtg ctgctccatc ctaagcgcca gcgtcttcgt cccgcgcagc accagccacg
     3601 cgtcgaaggg cgagatgatt gcccccacgc cgttctgcac gaactttatc cactcggcgt
     3661 cgcgctcgtc gttaaggacg acgcagccgc ccacgccgtc cgagtggccg ttcagatact
     3721 tggtcgtcga gtggacgacg atgtccacgc cgaagtccat cggccgctgg aggtagggcg
     3781 acatgaaggt gttgtcgcag acgacgcgcg cgcccgcgcg gtgcgctacg tccgagacgg
     3841 cctgcaagtc cgagacgatc atcacgggat tggtcggcgt ctccacgaag accatccgcg
     3901 tgttgtcgcg aatcgccgcc tcgacgttcg acgcgtcgga cgtatcgacg aaggtgaact
     3961 cgatgccgta gtttttgaga atcttgtcga agaggcggaa ggtgccgccg taggtgttat
     4021 cggagacgat gacgtgctcg ccgcccttga tgagccggag cgtggcgtcg atggcggcca
     4081 tccccgaggc gaaggcgtag ccgaaacgag cgccttcgag cgcggcgacg ttttgctcca
     4141 tcgccgtgcg cgtcgggttc tgcgtgcgcg cgtactcgta gcccttgttg cggccgaggc
     4201 cgtcctgcgc gtaggtcgag gtctggtaga tggggacggt gacggcgccc gtcgaggggt
     4261 caggctcgtt gccgacgtgg atggctgtag tggagaaacc cataagttcg tgaatcgtca
     4321 atcgtcaatc gtgaatcgcg gtcgcctctt tgctcaactc gaatggtaag tcgcaagtca
     4381 gggaaaattc aactttatcg atttacgatt cacgattcgc ggagttaatt gccgaatggt
     4441 agcccgccga ctttgctctg tcaaacccgt aacaagtgat aagtgataag tgatgagtga
     4501 taagaaaaaa cctttgctta gagctattag caattagctc ttatcactta tcacttatca
     4561 cttatcactt atcaccgttt tatcgcttgt cacttatcac cgtttccgat atgttgtagt
     4621 cccgtggata acacggagtc acggcgcacg tcgccgacgg tcgtcgccat catcccggcg
     4681 cgctacgcgt ccacccgact ccccggcaag cccctcctcg aaatcgcagg aaaaccgatg
     4741 attctgcacg tcgtcgagcg cgcgctcgcg gcgcggtgtg tcgcgcgcgt gctggtggcg
     4801 accgacgacg cgcgcgtcgc ggaggccgtg cgcggggccg gccgcgaggc cgtgttgacg
     4861 cgcgccgacc atcgctcggg cagcgaccgt ctggccgagg ccgtcgagag tgtggacgcc
     4921 gagatcgtcg tcaacttgca gggcgacgag cctttcgtct cgccgctgac ggtcgagcgc
     4981 gccgtcgcgg cgctcatcgc agacgagacg gcgcaagtct cgacgacgtg cgaggagatc
     5041 gagagcgcgg acgacgtgct caaccccggc gtggtcaagg tggtcacgga cgaacgcggc
     5101 tacgcgctct atttctcgcg cgcgcccgtc ccgttcccgc gcgacgcggc cctgcaccac
     5161 ggctcacttc aggccgcgct tgccaacgac ccgacgctgc tcaaaggctt tcgcaagcac
     5221 acgggacttt atgtttaccg ccgcgccttt ttgttagaat acgcgcgctg gccccagtcg
     5281 ccgctcgaac gcgccgaatc gctcgaacag ttgagaatcc ttgagcgcgg cgcgaagatc
     5341 aaagtcgtcg aggcggcttc cccctcaatc ggcgtggaca cgcccgaaga cctcgcgcgg
     5401 gtgcgcgcca tgatcgacgc gcaaagctca caggtggaac cgcggctcaa gtggccgggc
     5461 ttctgacctt gaaccttgaa agttgaacta cactgatggg cgactggatc ggactcggag
     5521 caatcattct cgtggcgttg ggcgccttct tcgggcttgc gcgtctgggc acgccggccc
     5581 ggccgatgac gcaggaagag ttcgagcaga aggtgcagga ggggcgcggg tggatgagcg
     5641 cgggtgcgtt cgcggggatg caggcgcttc agaagttgat gaacccgaag gcggccgagg
     5701 ccgtcgaggt gcagaaggac ttgcaggcgg gttactacga cgatcagcag gaggtgggcg
     5761 acggcgacga gcctgttgac cccgcggcag aagaagaaaa gcgaacgact gaagagggga
     5821 ctgatgccta aatacatttt cgtgacgggc ggcgtggttt cgagtctggg gaaggggctg
     5881 gcggcttcgt ccatcgggag cctgctcgaa gcgcgcggcc tgcgggtcac gcttatcaag
     5941 ctcgacccgt acatcaacgt cgatcccggc acgatgtcgc ccttccagca cggtgaggtc
     6001 ttcgtcaccg acgacggcgc cgaaaccgac ctcgacctcg gccactacga acgtttcacc
     6061 aacgcccggc tcacgcaggg caacaactgg acgacgggca gaatctacct ctccgtcatc
     6121 gagaaggagc gccgcggcga ctatctcggc aagaccattc aagtcatccc gcacatcacc
     6181 gacgagatca agcgggccgt gcgcgccgtc gcggagaaag acccggtgga cgtggtcatc
     6241 gtcgagatcg gcggcaccgt gggcgacatc gagagcctgc cgttcctcga agccatccgc
     6301 cagatgggca acgaggagcg gcaggacggc ggcaagggcg ccgtcttcgt ccacctcacg
     6361 ctcgtcccct acatcgccgc cgccggcgag ctgaagacga agccgacgca gcactccgtc
     6421 aaggagctgc gcgagatcgg catcgccgtt gacatcctgc tctgccgcag cgaccgcgcg
     6481 ctgtcgcgcg acctgtgctc gaagattgct ctcttctgca acgtcaggga gtcggccgtc
     6541 atcaccgcac aggacgtgga gaccatctac gaggtgccgc tggccttcca cgaacagggt
     6601 ttggacgaac ttatcgtcac ctcgctcggc ctcgaagagc gcgcggcgca atcagacctc
     6661 acgcgctggc ggcagctcgt ctcgaccatc aaggagccgt ccgccggctc ggtggccatc
     6721 gccatcgtcg gcaagtacgt cgagctggaa gactcctaca agtctctgcg cgaggcgctg
     6781 acgcacggcg gcgtggcgaa caacgtgcgg gtggacgtgc gctgggtcga gagcgagggg
     6841 atgatggatg aggactacga ggagcgcctg cgcgactacg acgccatcct cgtccccggc
     6901 ggcttcggca agcgcggcgt ggccggcatg attcgcgcca tcacctacgc gcgcaagtcg
     6961 aagacgcctt acttcggcat ctgtctgggg atgcagacgg cgaccatcga gtacgcgcgc
     7021 tcggtgtgcg gcctgctcgg ggcggattcc accgagttcg atgcggagac gccctatccc
     7081 gtcatcttca agctgcgcga cctcgtcggc gtcgaggaga tgggcgggac gatgcgcctc
     7141 ggcgaatatc cgtgcaacct cgcgccgggc agcctcgcgc gcgtggtcta cggcgggagc
     7201 gaatcaatcg gcgagcgcca tcgacaccgc tacgagttca acccggagtt ccgcgagcag
     7261 ttggagaggg cgggactggt cttcagcggc gagtcgccgg acgggcgctt cgtcgagatg
     7321 gtcgagctgc cgcgcgaggt tcacccgtgg tttctgggct gtcagttcca ccccgagtac
     7381 aagtcgaagc cgctcgcggc gcacccgctc ttcgcctcct tcgtccgcgc cgcctacgag
     7441 aaccggatgc gcgacgaggc gaccatcgag gacgcggccg aggcccccgc acacgagcac
     7501 gcgcacgtcg tcgccggcga cgactgatag aacacggata agacggatga gacggattgc
     7561 cacggattga tccgtactca tccgtcttaa tccgtgtcat acctggcctc ctcaccaagc
     7621 cccgaccaca tcaagtcaat gaatatcatc aaccccgaga tcgagaccga acgcctgctg
     7681 ctgcgcgcgt tcacgcccgc cgacttcgag gcgttcgccg cgatgcgcgc cgacgccgag
     7741 gtgatgcgtt tcatcgggat gcccgtgggg ccgcatacgc gggaacaggc gcgggcgtgg
     7801 caggagagga acactcggcg ctggcaggat aacggcttcg ggatgtgggc cgtcgtggag
     7861 aaggcgacgg gggaactagc cgggtggtgc ggactctccc ggctcgatga gacgcaggag
     7921 gttgaggtcg gctacggcct cgcgcgccgc gcatgggggc gggggctggc gaccgaggcc
     7981 gcccgcgcca gcctccgcta tggcttcgaa cggatgggtc tcacgcgcct cgtcgcggtc
     8041 gttaaccccg cgaaccacgc ctcccgtcac gtcatcgaaa agctcggcct gcgctacgtc
     8101 aaagacgcct accactacgg cgccgacctc aactatttcg agatcaaccg cgcggagttc
     8161 aagcccacgg ccacccccta cgttttgcgc gaaacgccgg caaacggtta aagtgatgaa
     8221 catcaaactc gaatgagaat catcgtcttc atcttaaccg cgctcgttca gtcggccgtc
     8281 gccgtggtga tttttttaat gctgctcctc ggcctcaacg ggttcagcga gtctgacgcc
     8341 actccgagcc tcatcttttt catcgccctg agcgtggtgt gtgcgctggg acttggcggc
     8401 gcggctgcct acacggcgaa gcggattgcc gagaaaaaat cgctcggcgg cgtcgcggct
     8461 tcggccatcg ccgtcctctg tttctcggtt ctcggcgggg tgattctggt cggcgggttt
     8521 tttgccgcgt tggtcatcgc aacgatcatg cacgagtgga agtaggggcg gccacacact
     8581 cgtcacttct cttatgctcc agtgtcccac atgcggccgg cagtttgcgg cggacgtgac
     8641 gaattgtccc gacgacgggg cgttgctgcg cgcggacgcg accatcgcca tcgatgcggc
     8701 cgccgcagac ccgctcgtgg ggcgcgtgct cgacgagaag tatcgactcg acgcgcgtct
     8761 gggcgagggc gggatgggga cggtctaccg cgccacgcac ctgctcatcg agcgtcccgt
     8821 ggcggtgaag gtcttgaact cgcgtctggt gacggacgag tcggcgcgcg agcggtttcg
     8881 gcgcgaggcg cgcgccgccg gtcggctcca gcacacgaac gccgtcgccg tgaccgactt
     8941 cggggagacg aaggacggcc tcgtctacct cgtcatggaa cttctggaag gtcggccgct
     9001 gcgcgaggtc gtcgcgcgcg aagcgccgct cgacccggcg cgcgccgtct cggtgatgct
     9061 tcaggtcagc gcggccgtcg ccgccgcaca cgaggccggc gtcatccacc gcgacctcaa
     9121 gcccggcaac atcttcgtcg tccagcgccc cagcgcgccg cacatcgtca aggtgctcga
     9181 cttcggcatc gccaagatcg ccgaggacgc cgacggcaac cccgccgaca cgttgacggg
     9241 gacaggcgtg atgatcggca ccccgcgtta catgtcgccc gagcagtgcg acggcgcgga
     9301 gctgacgccc gccgcggacg tttattcgct cggcgtcatc ctctacgaaa tgctcgccgg
     9361 gcagacgcca ttctccggcg ccaccccgct cgcgctcgcc ctcaagcatt catcggagac
     9421 gccgccgcgc ccctcggagt tcgcgcccgg catccagccc gcgctcgaag ccgtggtgct
     9481 ccacgcgctg gagaagtcgc cggacgagcg ccccgccgac gccggtgagt tccggcgcga
     9541 gttgttcgag gtggccgaac gactcggcct cgaacacgcc gagggcttca gctccccgac
     9601 catcgagacg ctgcgcgacg ccggcaccga gactccttcg ggccgcctcg tcatcgacat
     9661 cgaacgcctg cgccggcaac gtgccaccaa cacgctgccc gctgcccacg acaccgccga
     9721 ggaggaagcg gcgcgcacag ccggcagacg cgatttgcct ccgcccgacc cggcgcacgc
     9781 gggcgctatg ccgaaccggc ctcgccccgc cgtcgcgcgt cgagagagag tcgggcaaaa
     9841 caggttccgc gtgcgcgtcc ccacgaggcg gggggagcag tcgtgggcgt cgtatctgcg
     9901 gcagccgaac gtcgcgtggc tcgcgctcgc ggcggtactt ttcgtcggcg gtggcttcct
     9961 gctctatcgt gcgtt
//
LOCUS       DASVES010000090        13716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_43746, whole genome shotgun sequence.
ACCESSION   DASVES010000090 DASVES010000000
VERSION     DASVES010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43746"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1600
                     /locus_tag="VF240_03225"
     CDS             257..1600
                     /locus_tag="VF240_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX7174277.1"
                     /translation="MATKKSKPEIKNNHFEIPPADGKLGVLLVGLGAVSTTFIAGVEA
                     IKRGIAEPVGSVTQMGAIRLGKRTDNRVPLIKDFVPLARLEDLVFGAWDIFPDSAYDA
                     AMTAGVLEKELLTQLKPQLQKIKPMKAVFDQQYVKRLNGTNVKEGKNKFALAAQLIEE
                     IAEFKRKNRCARLVMVWCASTEIFMKPHGVHKTLKSFEQAMRDDHKAIAPSMIYAYAA
                     LKSGVPFANGAPNLTVDIPALRELAAEQGVPVCGKDFKTGQTLMKTILAPGLKSRMLG
                     LHGWYSTNILGNRDGEVLDDPESFKTKEESKLSVLEYILQPDVYPSLYKDFSHVVRIN
                     YYPPRGDNKEGWDSIDIFGWLGYPMQIKIDFLCRDSILAAPIVLDLALFLDLAQRVGM
                     KGVQEWLSFYFKSPMTAPTLYPEHDIFIQLMKLKNTLRHLRGEDLITHLGLEYYD"
     gene            2117..3304
                     /locus_tag="VF240_03230"
     CDS             2117..3304
                     /locus_tag="VF240_03230"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7174278.1"
                     /translation="MEKTTNTVVLDPDQKSPRAQEFEEKLGARIVGQERAVRRMSGLY
                     QIFLAGMNPPNRPVGTMLFLGPTGSGKTRVVEAAAEVLCGDQNAVIKIDCAEFQHSHE
                     IAKLIGSPPGYLGHRETSPMLTQENLDRYHTDDLKLSLVLFDEIEKASDSLWQLLLGI
                     LDKATLTLGDNRRVDFSRSMVIMTSNLGAREMSELISGGIGFAPAQGGKNPNDTEVDQ
                     KIYRTAVEAARRKFSPEFMNRIDKVVVFRSLKEHHLREILELELGAVQERIMRSAGTK
                     FVFHCSDGAKDMLLKEGIDFKYGARHLKRAIERFLVYPLSNLVATEQVGLGDLVHVDI
                     SESGKKLVFSKSSGGALINDAPREETDEHGGEAKSGGVGVPLPQATRAARRGQGRGDK
                     GEG"
     gene            complement(3400..4968)
                     /locus_tag="VF240_03235"
     CDS             complement(3400..4968)
                     /locus_tag="VF240_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174279.1"
                     /translation="MPAKRASKKSSGGGASKSARKAAPSSRAAKKSAGGKSAAKKTSG
                     KKPGARKGVAGKKGAAKRTAAPKVLAPGDIMIVNMIPRSLSNETHQDSEPHLTVNPNN
                     PSQIVGSAFTRNPFGGPQAPIYVSNDGGRTWSLEFVVPSQSITGDITVGFSGSSQLYT
                     GILRLPGSLRQSILRTNTPSGPTPMNVLVDRANVDQPFVQATTFGSRDRVYVGNNDFT
                     ATGGRTATIEQSTDAARPTPTFTKVRIEARGTGSARQDGPQVRPVCHRDGTVYAVFYG
                     WRAFNEGNNEVTADVVVVRDDLGGTGATQFKSLRDAGDGLAGKRVAQGVRFIWADTLG
                     QQRMGGDVSIAADPRNSGTVYVAWGDVRPGTGYTLHVRRSTDRGVTWSPSDLRTIAKA
                     TNPSLAINSQGVVGLLYQQVTGTGAGARWVTQLDRSLDGVNWSNLVLARVPAASPLPQ
                     FQPYIGDYDHLVSVGRDFLGIFSANNTPDRNNFPNGVTYQRNHDFTTRRLLANNNTTQ
                     VGVSIDPFFFKVSG"
     gene            complement(5064..5864)
                     /locus_tag="VF240_03240"
     CDS             complement(5064..5864)
                     /locus_tag="VF240_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174280.1"
                     /translation="MTRVQVSDKTIVVRVDEVLQAPETLEDLTGTDITVELSGRQKVK
                     AGDEAIFYTNGWLFGEGIAVRAVGVRPVKKATRAMLGMSGVAPPKNLRQQKMEERVSS
                     ADLVVTGRVTDVRLPADEGGGPMTAGRSTKRSAKAGAAAPGAAKPVRRRPITEHDPQW
                     REAVVEVADVEKGSQKKKVVIRFPSSNDVRWYRAPKFEPGQQGVFILHKTDAAAKPQK
                     PRLRATVTPAEAETSEAYTCLAPEDFQPIQKSSEIKTLVGGANKPDEE"
     gene            complement(6250..7170)
                     /locus_tag="VF240_03245"
     CDS             complement(6250..7170)
                     /locus_tag="VF240_03245"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7174281.1"
                     /translation="MSRRLHPTRRHAPPNSRAAIAIAAATVLFLPPPPAPRKASALGL
                     HPLAAVTTQSSDTLTPLQRQIETQSRRLASVDVEERRDAVVSLGALRRPEASRAAGAA
                     LGDRSAVVRAHATRAVLSLAPADGAALLAPVLLRDRDEFVRRESAYALGLARAGAAVP
                     ALVNALERDKQASVRGAAAVALGQIGDPVAVSVLARKLSERIPSTGLLSRVTRGKREE
                     DEFVRRAAAVSLGQIGDRAAAPALIDTLSKERSEDDVRREAARALGLIGDPAAIPALR
                     AALNARDPHLSRIAQEALRKLSLGVPSPFM"
     gene            complement(7167..7430)
                     /locus_tag="VF240_03250"
     CDS             complement(7167..7430)
                     /locus_tag="VF240_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174282.1"
                     /translation="MAKRINERQRLALKVDDLNESEVREVLEYISIMESMRRARAMPG
                     LWEDELVAILADTPENVRARQAFEWEVVRRRAERRAAAAPARI"
     gene            complement(7872..8687)
                     /locus_tag="VF240_03255"
     CDS             complement(7872..8687)
                     /locus_tag="VF240_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7174283.1"
                     /translation="MVDYYKVLGVKRTATDIEIKSAYRKLARQLHPDVNGGTEKASRQ
                     FALVALAYRTLSDPQERAFFDAQLKKLRTGFQTGKESVFSTANPHAQRMRRAAVQSRF
                     DGIVDRLIEAERRETFALQQAVFTTVTLFLSTFFVALFKPRLWDNFRGVGRAVLVTLF
                     LIGLWHLAVRLKACFDHYTYKPRSIHDSIINARDDKTDKPFTRFAASAFLVVGYAASL
                     AAGLFVGSQAHEVVIFQDLPLLFDRQIRPDLIFYPPIAVLIVDSMHMLASKID"
     gene            complement(9140..10684)
                     /locus_tag="VF240_03260"
     CDS             complement(9140..10684)
                     /locus_tag="VF240_03260"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX7174284.1"
                     /translation="MNIETNVGGYAETDVQALAVAVFKDEKADDGFLKELDAATGGLV
                     RSVLEAEEMKGKEGETAYVHLSGSEGLKAKRLLLVGAGERDSYGLGQVAHLAGAAVRY
                     LRARNVKSIGLAPRVPETLDAARAASAAAEGAVMGLFDMDKYRTVDKEEKNIDRFVVI
                     MEGADEGAVRGGGERGRIVGESVNFTRDLANEPGAYMTPTILAERAEQIASEFGLEVE
                     ILEQSDMEAEGMGALLSVARGSEQVPKLIILKYMPAGKAKIEDGEDYLAIVGKGITFD
                     SGGISLKPGENMELMKYDMTGGATVLGVMRAIAQLKPSVPILGVVPATENMPSGKATK
                     PGDIVRAMSGKTIEVINTDAEGRLILADAVSYAKKLGATRIIDMATLTGAVSIALGDV
                     NTAILGTDQQLIDDFVESGREVGEKFWQLPLDKEYSKQIKSDIADIKNVGGRKAGTIT
                     AAAFIKEFTDSTPWAHLDIAGTAWGDEAKPFRSKGPTGVAVRTLINFIERSGNHKPAE
                     GKKAEK"
     gene            complement(10705..11169)
                     /gene="smpB"
                     /locus_tag="VF240_03265"
     CDS             complement(10705..11169)
                     /gene="smpB"
                     /locus_tag="VF240_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX7174285.1"
                     /translation="MAEGEKVIVTNRAALHEYHVQDRYEAGIALTGTEVKSVRAGQIQ
                     LKDSYVAVREGEAWLFNAHISPYSHGNRENHDPVRTRKLLLHRREIDRMSKETELKGM
                     TLVTTRVYLKGGRVKFEIGVARGKKLYDKRETEMRRTVERETRAQIKERDRS"
     gene            complement(11179..12207)
                     /locus_tag="VF240_03270"
     CDS             complement(11179..12207)
                     /locus_tag="VF240_03270"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7174286.1"
                     /translation="MLRVAVMGGGGWGTALAVVAGRAGHEVRLWSRNAGVVEEVNARR
                     VNDTYLPGCEVPPNVRATTDIAEALSGAEFVVLAAPSHATRSLLSALAGHARPRMIFV
                     SATKGIEVETGKRISEVAAEVLGAGTEGLRFVCLSGPSFAQEVAAGQPTAVVAASDEK
                     ELARSVQAELSAPNFRIYTNTDVVGTELGGALKNVMALASGMVAGLGYGTNSVAALIT
                     RGLSEMTRLALAEGARPETLMGLAGLGDLVLTCTGGLSRNRRVGQELGRGRALAEVLA
                     GMKEVAEGVKTTRAVMLLAARLGVEMPITREVNAVLYEGKSARSACEELMSRPLKGEF
                     EGQVEQGA"
     gene            complement(12298..12915)
                     /gene="plsY"
                     /locus_tag="VF240_03275"
     CDS             complement(12298..12915)
                     /gene="plsY"
                     /locus_tag="VF240_03275"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX7174287.1"
                     /translation="MMPSLVILAAYLLGSIPFGYLIVRLASGGDVRATGSGGTGATNV
                     TRRAGKKAGLLTLALDALKGALAVLLARALLTEDFGANWWVAGAAVASVLGHCFPVWL
                     RFRGGKGVATGLGVFMALAPLAVLFSALPVFLLVVWATRYVSLGSITAAALLPLCVWL
                     LGGYTYPPEQRAPLTSAAAAGAALIVLMHRANIGRLLKGTESKLK"
     gene            complement(12926..13594)
                     /locus_tag="VF240_03280"
     CDS             complement(12926..13594)
                     /locus_tag="VF240_03280"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX7174288.1"
                     /translation="MNQKETEPGLSFERVVFFSDAVFAIVITLLVLEIKVPHLEGHTE
                     RDVGRGLLHLLPKFLGFVMSFFVVGSMWIEHHRIFRLIDSYDIGLLFRNLLLLLSVSF
                     IPFPTALYSEYSWSRTAFILYAASFGVVALMKLWVWRYAASRPALLSAAADAQTIRRV
                     SRRSWAVPAACAVAIALSFISVYLAPLGFPFIPLFARLLDPAPPGVAADLSGDVAGDE
                     TPAA"
BASE COUNT         2329 a         4428 c         4445 g         2514 t
ORIGIN      
        1 gccaccgccc tcaaagccca actcgcccgc aaaggcatca agtaaggcaa gtaagcagta
       61 agcagtaagc agtgagcagt gagcagtgag cagtgagcag tcaacgccag tagcgcgtcc
      121 tgactgctca ctgctcactg cttactgctt actgtcttca gcaatccccc accgttttac
      181 cgcaagatat gacaagacgc ggcgacagcg tctccggtat gctcgacgaa atagattcga
      241 gaaggagtgt gctcgcgtgg caactaaaaa atccaagccc gagattaaaa ataatcattt
      301 cgagattccg ccggccgacg gcaagctcgg tgtattgctc gtcggcctcg gcgccgtcag
      361 caccaccttc atcgccggcg tcgaggccat caagcgcggc atcgccgagc ctgtggggag
      421 cgtcacccag atgggcgcca tccggctcgg caagcgcacc gacaaccgcg tccccctcat
      481 caaagacttc gtcccgctcg cccggctcga agacctcgtc ttcggcgcct gggacatctt
      541 ccccgactcg gcctacgacg cggcgatgac ggccggcgtc cttgagaagg aactgctcac
      601 gcagctcaag ccgcaactcc agaagatcaa gcccatgaag gccgtcttcg accagcagta
      661 cgtcaagcgc ctcaacggca cgaacgtcaa ggagggcaag aacaagttcg ccctcgccgc
      721 tcagctcatc gaggagatcg ccgagttcaa gcgcaagaac cgctgcgcgc gcctcgtcat
      781 ggtctggtgc gcctccaccg aaatcttcat gaagccgcac ggcgtccaca agaccctgaa
      841 gtcgttcgag caggcgatgc gcgacgatca caaggccatc gccccgtcga tgatctacgc
      901 ctacgccgcg ctcaagtccg gcgtcccctt cgccaacggc gcgcccaatc tcaccgtgga
      961 catccccgcg ctgcgcgagc tggccgcgga gcagggcgtc cccgtctgcg gcaaggactt
     1021 caagaccgga cagacgttga tgaagaccat cctcgcgccc ggcctcaagt cgcgcatgct
     1081 cggcctccac ggctggtact ccacgaacat cctcggtaac cgcgacggcg aagtcttaga
     1141 cgatcccgag tcgttcaaga ccaaggaaga gtccaagctc tcggtgctcg aatacatcct
     1201 ccagccagac gtttacccgt cgctctacaa ggacttctcg cacgtcgtcc gcatcaacta
     1261 ctatccgccg cgcggcgaca acaaggaggg ctgggacagc atcgacatct tcggctggct
     1321 cggctacccg atgcagatca agatcgactt cctctgccgc gactccatcc tcgccgcccc
     1381 catcgtctta gacctcgccc tcttcctcga cctcgcgcaa cgcgtcggga tgaagggcgt
     1441 acaggagtgg ctctccttct acttcaagtc gccgatgacg gcgccgaccc tctaccccga
     1501 gcacgacatc ttcatccaac tgatgaagct caagaacacc ctgcgccacc tgcgcggcga
     1561 agacctgatt acccacctcg ggcttgaata ctacgactga cgcctgtcgg tcgcgcagcg
     1621 aatatcaggg gcgtgtccgc cgtcaggaca cgcccctttt ttatataaac cattccgtcc
     1681 ctacgcatag attcggcaga cacgctttgc tacacccgga acttccgggc cgacgtttaa
     1741 gtgcgcggtc gcgcagggtt gcccgtccaa tttttcggag aacgactgtt cgtgtcaccc
     1801 tccgactgcc gtaatctgtc aggggcacgc ttgtcctccc ctgcctaaaa aactttgtcc
     1861 ggcgagacca tcgcatattt tgtggtgaca aaagagtcgt tgtcgtatat atagtgctct
     1921 cgaatttatg cgcgcccctc ggggctccga aaggagttac aaccgcctcc cgaggccgcg
     1981 catcaactat gttgcggttc ggtctttgca tggggtgtgg ggtagcgggc cggcgccggc
     2041 taaatgataa gggcgcccca gccagcgcct ttgagtcgct cctgcaagga ggacgaacat
     2101 gcttgcagaa atggaaatgg agaaaacaac gaacacggtc gtactcgatc ccgaccagaa
     2161 gagtccgcgc gcgcaggagt tcgaggagaa gttgggcgcg cgcatcgtcg ggcaggagcg
     2221 cgcggtgcgc cgcatgagcg gcctctacca gattttcctc gccgggatga atccgccgaa
     2281 tcgccccgtc ggcacgatgc tcttcctcgg cccaacgggg tcgggcaaga cgcgcgtcgt
     2341 cgaggccgcg gccgaggtgc tgtgcggcga tcagaatgcg gtcatcaaga tcgactgcgc
     2401 tgagttccag cactcgcacg agatcgccaa gctcatcggc agcccgccgg gctacctcgg
     2461 ccaccgcgag acctctccga tgctcacgca ggagaacctc gaccgctacc acaccgacga
     2521 cctcaagctc tcgctcgtcc tcttcgacga gatcgagaag gccagcgact ccctgtggca
     2581 gctcctgctc ggtatcctcg acaaggcgac gctgacgctg ggcgacaacc gccgcgtaga
     2641 cttctcgcgc tcgatggtca tcatgacctc gaacctcggc gcacgcgaga tgtcggagct
     2701 gatctcgggc ggcatcggct tcgcccccgc gcagggcggg aagaacccga acgacaccga
     2761 ggtcgatcag aagatttacc gcacggcggt cgaggcggcc cggcgcaagt tctcgccgga
     2821 gtttatgaac cgcattgaca aggtcgtggt cttccgcagt ttgaaggagc accacctgcg
     2881 ggagattctg gagttggagc ttggggccgt gcaggagcgc atcatgcgca gcgcgggcac
     2941 gaagttcgtc ttccactgct cggacggagc caaggacatg ctcctcaagg agggcatcga
     3001 cttcaagtac ggcgcgcgcc acctcaagcg ggccatcgag cgcttcctcg tctacccgct
     3061 ctcgaacctc gtggcgaccg agcaggtggg cctcggcgac ttagtccacg tggacatctc
     3121 cgagtcgggc aagaagctcg tcttctcgaa gagttcgggc ggcgcgctca tcaacgacgc
     3181 cccgcgcgag gagacggacg agcacggcgg cgaggccaag tcgggcggcg tcggtgtgcc
     3241 gctcccgcag gcgacgcggg ccgcccgccg cggccaggga cgcggcgaca agggtgaagg
     3301 ctgacagccg gctaacccac ccatcaaaag aacaagagcc ggagagcgat gtgaatcgtc
     3361 tctccggctc ttgttctttt gatggggtct tcccgcgtct tagcccgaca ccttgaagaa
     3421 gaacgggtcg atggagactc cgacctgggt cgtgttgttg ttggctaaga gcctgcgggt
     3481 cgtgaagtcg tggttgcgct ggtaggtcac cccgttggga aagttgtttc tgtccggtgt
     3541 gttgttggcc gagaagatgc cgaggaagtc gcggccgacg ctgacgaggt gatcgtagtc
     3601 gccgatgtag ggctggaact gcgggagcgg ggaggcggcg ggcacgcgcg ccagcacaag
     3661 gttgctccag ttaacgccgt cgagcgagcg atccagttgc gtcacccagc gtgcgcccgc
     3721 gcccgtgccc gtgacctgct ggtagagcag gccgacgacg ccctgactat tgatggcgag
     3781 cgaagggttg gtggccttgg cgatagttcg caaatcactc ggcgaccagg tgacgccgcg
     3841 atccgtcgag cggcgcacgt gcagcgtgta gcccgtgccg ggccgcacgt cgccccacgc
     3901 gacgtagacc gtgccgctgt tgcgcgggtc ggcggcaatc gacacgtccc cgcccatgcg
     3961 ctgctggccg agtgtatcgg cccagatgaa tctgacgccc tgcgcgacgc gcttgccggc
     4021 cagcccgtcg ccggcgtcgc gcagagactt gaactgtgtg gcccccgtcc cgcccaggtc
     4081 gtcgcggacg acgacgacat cggcggtgac ttcgttattg ccctcattga aggcccgcca
     4141 gccgtagaag actgcgtaga ccgtcccgtc gcggtggcac acgggccgga cttgcgggcc
     4201 gtcttgcctg gcgctgcccg tgccccgcgc ctcgatgcga accttcgtga aggtcggcgt
     4261 cgggcgcgcc gcgtccgtcg attgttctat ggtggccgtg cgcccgcccg tcgcggtgaa
     4321 gtcgttgttg ccgacgtaga cgcggtcgcg gcttccgaag gtcgtcgcct gcacgaaggg
     4381 ctgatcgaca ttcgcgcgat ccacaaggac gttcatcggg gttggcccgg aaggcgtgtt
     4441 ggtgcggagg atgctttgtc ggaggctacc ggggaggcgg aggatgccgg tgtagagctg
     4501 gctcgaaccg ctgaagccga ccgtaatgtc tcccgtgatg ctctggctcg gcacgacgaa
     4561 ttcgagcgac cacgtgcggc cgccgtcgtt ggagacgtag atgggcgcct gcggcccgcc
     4621 gaacgggttg cgcgtgaacg cggagccgac gatctgcgaa gggttgttcg ggttaaccgt
     4681 caggtgcggc tcgctgtcct gatgcgtctc gttactcagc gaacgcggga tcatgttgac
     4741 gatcatgatg tcgccggggg cgagcacctt cggcgcggcc gtccgcttgg ccgcgccctt
     4801 cttgccggca acgcctttgc gcgcgcccgg cttcttgccc gaggtctttt tcgcggcgct
     4861 ctttccgccg gcagattttt tagcggcgcg cgaggagggg gcggccttcc gggccgactt
     4921 gctcgccccc ccgccggaag attttttgct cgccctcttg gctggcataa gcttctctcc
     4981 ttctgcctgt cagctgaaga ccgcgcgcca ctgtaagtgg gtcggtgttg acgcgccacg
     5041 gcagtccgac ggccgtcgtg agtctattcc tcgtccggct tgttcgcgcc gccgacgagg
     5101 gtctttatct cggagctttt ctgaatcggc tgaaagtctt cgggggcgag acaggtgtag
     5161 gcctcggatg tctcggcctc ggcgggggtg acggtggccc tcagacgagg cttctgcggc
     5221 ttggcggccg cgtccgtctt gtgaaggatg aagacgccct gttgacccgg ctcgaacttc
     5281 ggagccctgt accagcgcac gtcgttgctg ctcgggaaac ggatgacgac tttcttcttc
     5341 tgactgccct tctccacgtc cgccacttcc accacggcct cgcgccactg cgggtcgtgc
     5401 tccgtgatcg gccgccgcct gacgggcttc gccgcgccgg gagccgccgc ccccgccttg
     5461 gccgaccttt tcgttgaccg gccggcggtc atggggccgc cgccctcgtc cgcgggcagc
     5521 cgcacgtcgg tcaccctgcc ggtgacgacg aggtcggcgc tcgaaaccct ctcctccatc
     5581 ttctgctgtc ggagattttt cggcggcgcg acgccactca tgccgagcat cgcccgcgtg
     5641 gccttcttca cggggcgcac gccgacggcg cgcaccgcga tgccttcccc gaacagccag
     5701 ccgttggtgt agaagatcgc ctcgtcgccg gccttgacct tctgacggcc gctcaactcc
     5761 accgtgatgt cagtccccgt caggtcttcc agcgtttcgg gggcttgcag gacttcgtct
     5821 acgcgcacca cgattgtctt gtcggagacc tggacgcggg tcatggtagc cgccttcagc
     5881 ttttgcaccg tgccgcgaaa aacgaacccg gactctcgga tcaattcttc ctcactcaaa
     5941 gttggcatct tcctctcctc tcggcgttcg acccttaccg agcgacgcgg tctttaacga
     6001 aaaacttatg cggccccatc caaaaacctg ctctgcacgg gttgtattct agacccttgc
     6061 tgccgtccct gcacgccact cgtaacttcg cgcgtcgccg gaggtctata agctgacgaa
     6121 agcgggactc gtctttcctc ggaacagcgc tgccgacccg acgggataat tcctatgtcc
     6181 tactcatcat gccggaacgc gagaaaatat ttcatggcga cgaacggacg gccgtagaag
     6241 tcagttccgt cacataaacg gtgacggcac gccaagactg agtttgcgga gcgcctcttg
     6301 ggcgatgcgc gaaaggtgtg ggtcacgcgc gttgagcgcg gcgcgcaggg ccgggatggc
     6361 ggccgggtcg ccgatgagtc cgagtgcgcg ggcggcctca cgcctcacgt catcctcgga
     6421 gcgctccttc gagagggtgt cgatgagggc cggcgccgcc gcgcggtcgc cgatctggcc
     6481 gagcgagacg gccgccgcgc ggcgcacgaa ctcatcctcc tcacgcttgc cgcgcgtcac
     6541 gcggctcagt agtccggtac tggggatgcg ctcagaaagt ttccgcgcca gcacggagac
     6601 ggccacgggg tcgcctatct gcccaagcgc cactgccgcc gccccgcgca ccgacgcctg
     6661 cttgtcgcgc tccaacgcat tcaccagcgc gggcacggcc gccccggcgc gcgccaggcc
     6721 gagcgcatag gccgactcac ggcgcacgaa ctcatcgcgg tcgcggagca agaccggcgc
     6781 gagaagtgcg gccccgtcgg ccggagcgag cgagaggact gcgcgggtcg cgtgcgcgcg
     6841 cacgacggcc gagcggtcgc ccagcgccgc gccggccgcg cgcgaagcct ctggacgccg
     6901 caacgcgccg aggctgacga cggcgtcgcg tcgttcttcc acatcgaccg acgcaagcct
     6961 tctgctttgc gtttcgattt gtcgctggag cggggtcagt gtgtccgagg attgggtggt
     7021 gacggcggcc agcggatgga ggccgagggc tgaagcctta cgcggggccg gcggcggagg
     7081 gagaaagagc accgtggccg cggcgatggc aatcgcggcg cggctgttgg gtggcgcgtg
     7141 tcttcgggtc ggatgaaggc gtcggctcat atacgggcgg gcgcggcggc ggctcgtcgc
     7201 tctgcgcggc ggcgcacgac ctcccactcg aacgcctgac gggcgcgcac gttctccggc
     7261 gtgtcggcca gtatcgcgac gagttcgtcc tcccacaggc ccggcatcgc tctggccctg
     7321 cgcatggact ccataattga gatgtattcg aggacttctc gcacctccga ctcgttgagg
     7381 tcgtctacct tgagggcgag gcgctggcgt tcgttgattc gcttggccat cttctggctc
     7441 cttcgtcggc gaaaccggac ggccaaagcg cgggcggtca actctgcgga aggattcggg
     7501 agcgaccggc cgacgggccg tcggcgatca ggtgtggcgc gtatggtgcg cgtctgtttt
     7561 tcttcacttg tgtcctgaga ttctccgggt gtagcaaact cttctgaagt cttgggcgcg
     7621 taactttaag ttatccgaac gaccgccctc gccaatcgcc gccacgcgtt gccatgtcgc
     7681 gggtcggaga gtattgaact ctcgcccgct ttgccggtca cgtcagttgt cggttgcgaa
     7741 ttttgcggcg gccgtcaagg ctcgctatgg gcgcaatata cacgagggcc gtggggggcg
     7801 caagatttgt tccccctcgc caaaagaccc ggcggggctg tttctggcgc tgcctgacgc
     7861 tatggatttt cctagtcgat cttcgaggcg agcatgtgca tcgagtcaac gatgagcacg
     7921 gcgatgggcg ggtagaagat gaggtcgggg cgaatctgcc ggtcgaagag gagcgggagg
     7981 tcctggaaga tgaccacctc atgcgcctgc gagccgacga agaggccggc ggcgagactc
     8041 gccgcgtagc ccacgacgag gaaggccgag gccgcaaacc gcgtgaaggg tttatctgtc
     8101 ttgtcgtcgc gggcgttgat gatcgagtcg tgaatcgaac ggggcttgta ggtgtagtgg
     8161 tcgaagcacg ccttgagccg gacggccaga tgccacagcc cgatgaggaa cagcgtcacc
     8221 agcacggcgc ggccgacgcc gcggaagttg tcccacaggc gcggcttgaa gagcgccacg
     8281 aagaaggtcg agaggaagag tgtgaccgtg gtgaagaccg cctgctggag cgcgaaggtc
     8341 tcgcgccgct cggcctcgat caggcggtca acgatgccgt cgaagcgcga ctgcacggcc
     8401 gcccgccgca tccgctgggc gtgcgggttc gcggtggaga agaccgactc tttgccggtc
     8461 tggaagccgg tgcggagttt cttgagctgg gcgtcgaaga aggcgcgctc ctgcgggtcg
     8521 ctgagcgtgc ggtaggcgag cgcgacgagg gcgaactggc gggaggcttt ctcggtgccc
     8581 ccgttgacat cggggtggag ttggcgcgcg agcttacggt aggccgactt gatctcgatg
     8641 tccgtggccg tccgcttcac ccctaagact ttgtagtagt caaccaccgc gctttccctg
     8701 aaattgccaa agcaggcgat ggaacgctct tccgtgtggg aacgctcaat cgtacttttt
     8761 cttcgggtaa gggagggcac ccgcaggcgt aatatagcat ttttcgcaga cattcgtccc
     8821 gcctaattcg ccgggccgtg aggggcgtca ggcgacaggg ggacgaatgc tgcggaggtg
     8881 aggacagtta ccgggaagtg gtgctttctc ctcgtgcggc caccgagtgg ggcgaggaca
     8941 ctttttagtg gtgaccactt cagccgggtt gctgagtggc tgtattccaa cattgatgac
     9001 aattttttgc aagcaaaaaa tccacatgtg ggagaagtat ttacgctacc gcccacggtc
     9061 gcccccacga agaaggacgc cggcgtcaac aatcgtcaac gccggcgtcc gtcggagctt
     9121 tattatctcg ggaaaactct tatttttccg cttttttccc ttcggccggc ttgtggttgc
     9181 cggaacgctc gatgaagttg atgagcgtgc ggacggcgac gcccgtcggc cccttcgagc
     9241 ggaagggctt ggcctcgtcg ccccaggccg tgccggcgat gtcgaggtgc gcccagggcg
     9301 tgctgtcggt gaactccttg atgaaggcgg cggccgtaat cgtcccggcc ttgcggccgc
     9361 cgacgttctt gatgtcggcg atgtccgact tgatctgctt ggagtattcc ttgtcgagcg
     9421 ggagctgcca gaacttctcg ccgacctcgc ggcccgactc gacgaagtcg tcgatgagct
     9481 gctggtcggt gccgaggatg gcggtgttca cgtcgccgag ggcgatggag acggcgccgg
     9541 tcagggtcgc catgtcgatg atgcgcgtgg ccccgagctt cttggcgtag gagacggcgt
     9601 cggcgaggat gagacggccc tcggcgtcgg tgttgatgac ctcaattgtc ttgccggaca
     9661 tggcgcggac gatgtcgccg ggcttggtgg ccttgcctga aggcatgttc tcggtggcgg
     9721 ggacgacgcc gaggatgggc accgacggct tgagctgcgc gatggcgcgc atcacgccga
     9781 ggacggtggc gccgcccgtc atgtcgtact tcatcagctc catgttctcg ccgggcttga
     9841 gagagatgcc gcccgagtcg aacgtgatgc ccttaccgac gatggcgagg tagtcttcgc
     9901 cgtcttcaat cttcgccttc ccggcgggca tgtacttgag gatgattagc ttcggcacct
     9961 gttcggagcc gcgggcgacg ctcaggagcg cgcccatgcc ctccgcctcc atgtccgact
    10021 gttcgaggat ttcaacctcc agtccgaact ccgaggcgat ctgctcggcg cgctcggcga
    10081 ggatggtcgg ggtcatgtag gcgcccggct cgttggcgag gtcgcgcgtg aagttgaccg
    10141 actcgccgac gatccgtccg cgttcgccgc cgcccctgac ggccccctca tccgcacctt
    10201 ccataatcac gacgaagcgg tcgatattct tttcctcctt atcgaccgtg cggtacttgt
    10261 ccatgtcgaa gaggcccatg acggcgccct cggcggcggc ggaggcggcg cgggcggcgt
    10321 cgagagtttc ggggacgcgc ggggcgaggc cgattgactt gacgttgcgc gcgcgcaggt
    10381 aacgcacggc ggcgccggcc aggtgcgcga cctgcccgag accgtacgag tcgcgctcgc
    10441 ccgcgccgac gagcagcagg cgcttcgcct tgagtccttc cgaaccggag aggtggacgt
    10501 aggccgtctc gccctccttg cccttcatct cctccgcctc aaggaccgag cggacgaggc
    10561 cgcccgtcgc cgcgtcgagt tctttgagga acccgtcgtc ggccttctcg tctttgaaaa
    10621 cggcgacggc cagggcttgc acgtcggtct cggcgtagcc tcccacgttg gtttcgatat
    10681 tcatgcttta atcatctcct ctgctcaact tctgtcgcgc tccttgattt gcgcgcgcgt
    10741 ctcgcgctcg acagtccggc gcatctccgt ttcacgcttg tcgtatagtt ttttaccacg
    10801 cgcgacgccg atttcaaatt tgacgcggcc ccctttgagg tacacgcgtg tggtgacgag
    10861 cgtcatccct ttaagctctg tctccttcga catgcggtcg atctcccgcc ggtgcaggag
    10921 cagcttgcgc gtgcggacgg ggtcgtggtt ctcacggttg ccgtgcgagt agggcgagat
    10981 gtgcgcgttg aagagccacg cctctccctc gcgcaccgcg acgtaagagt ctttgagctg
    11041 gatttggccg gcgcgcacgc tcttgacctc cgtccccgtc aacgcgatgc cggcctcgta
    11101 cctgtcttgc acgtgatact cgtgcagcgc cgcgcggttc gtgacgatta ccttctctcc
    11161 ttccgccatg attctccctt acgctccctg ttcgacttgc ccctcgaact cgcccttgag
    11221 cggcctcgac atcaactctt cgcaggctga gcgcgccgac ttaccttcgt agaggacggc
    11281 gttcacctcg cgcgtgatgg gcatctcgac gccgagccgc gcggcgagca gcatcacggc
    11341 ccgcgtcgtc ttcacgccct cggccacctc cttcatcccg gcgagaactt ctgcgagcgc
    11401 gcggccgcgg ccgagttcct gaccgacgcg gcggttgcgt gagagtccgc cggtgcatgt
    11461 caggacgagg tcgcccaagc ccgcgaggcc catcagggtt tcggggcgcg cgccctcggc
    11521 gagggcgagg cgcgtcatct cggacagacc acgcgtgatg agcgcggcga cgctgttcgt
    11581 cccgtagccg agtccggcga ccatccccga agccagcgcc atgacgttct tgagagcgcc
    11641 gcccagctcc gtgccgacca cgtcggtgtt agtgtagatg cgaaagttcg gcgcgctcaa
    11701 ctccgcctgc acgctgcgcg ccaattcttt ctcatcgctt gcggcgacga cggccgtcgg
    11761 ctgccccgcc gccacctcct gcgcgaacga cggccccgac agacagacga agcgcaaccc
    11821 ctccgtcccc gcgcccaaaa cctcggccgc cacttccgag atgcgcttcc ccgtctcgac
    11881 ctcgatgccc ttcgtcgcgc tgacgaagat catccggggc cgcgcgtgcc cggcgagggc
    11941 ggacagcaga gagcgcgtcg cgtgcgaagg ggccgcgagc acgacgaact cggcgccgct
    12001 tagagcctcc gcgatgtccg tcgtcgcgcg cacgttcggc ggcacctcgc accccggcag
    12061 gtatgtgtca ttaacccgac gtgcattgac ctcctcaacg acacccgcgt tgcgcgacca
    12121 caagcgcacc tcgtggcccg cccgcccggc gacgacggcc agcgccgtcc cccatccccc
    12181 accgcccatg actgcgactc taagcataat gacagtagtg ataagtgatg agtgataagt
    12241 gatgagaagg aagcaactgc ctcttatctt ttcttatcac tgatcactca tcacttatca
    12301 cttcagcttg ctctccgtcc ccttcagaag ccggccgatg tttgcgcggt gcatgaggac
    12361 gatgagtgcg gcgccggcgg cggcggccga cgtgagcggc gcgcgttgct cgggcgggta
    12421 agtgtagccg ccgaggagcc agacgcaaag cggcaacagg gccgcggccg tgatcgagcc
    12481 gagtgagacg tagcgcgtcg cccacacgac gaggaggaag acggggagcg cgctgaagag
    12541 cacggcgagc ggcgcgaggg ccatgaaaac gccgaggccc gtcgccacgc ccttgccgcc
    12601 acggaagcga agccacacgg ggaaacagtg tcccagaacg gacgccactg cggccccggc
    12661 cacccaccag ttggcgccga agtcttcggt caacagggcg cgggcgagca ggacggcgag
    12721 cgcgcccttg agcgcgtcga gcgcaagcgt cagcaggccc gccttcttcc ccgcgcgccg
    12781 ggtaacgttc gtcgcgcccg tcccgcccga gccggtggcg cgcacgtcgc cgccgctcgc
    12841 gaggcgcacg atcagatagc cgaaaggtat cgagccgagc aggtacgcgg cgaggatgac
    12901 gagtgagggc atcattggtc gtttgtcacg cggccggggt ctcgtctccc gcgacatctc
    12961 cgctcagatc ggccgccacg cccggcgggg cggggtcgag caggcgggcg aagaggggga
    13021 tgaacgggaa gccgagcggg gcgagataga cgctgatgaa ggagagcgca atcgccacgg
    13081 cgcaggcggc cggcaccgcc cacgaacgcc ggctgacgcg ccggatggtc tgtgcgtcgg
    13141 ccgccgcgct caggagcgcg ggccgcgacg cggcgtagcg ccagacccag agtttcatca
    13201 gcgcgacgac gccgaagctc gcggcgtaga ggatgaacgc cgtgcggctc caggaatatt
    13261 cgctgtagag cgccgtcggg aacgggatga aggagacgct cagcagcagc agcaggtttc
    13321 tgaagaggag gccgatgtcg taggagtcga tgaggcggaa gattctgtga tgctcgatcc
    13381 acatcgaccc gacgacgaag aagctcatga cgaagccgag gaacttgggc agcagatgca
    13441 gcaggccgcg ccccacgtcg cgctcggtgt gcccttcgag atgggggact ttgatttcga
    13501 ggacgagcag cgtgatgacg atggcgaaga cggcgtcgct gaaaaagacg acgcgctcga
    13561 agctgaggcc cggttcggtc tctttctggt tcacggttca ctccagaggg gattggttca
    13621 ttgattcatc ggttcattga ctcatttaaa tcagtaccgc gagcggtagc gagcgggtcg
    13681 tgccttgcgc aaccacaccc gctcgctacc gctcgc
//
LOCUS       DASVES010000091         7105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1013511, whole genome shotgun sequence.
ACCESSION   DASVES010000091 DASVES010000000
VERSION     DASVES010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013511"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF240_03285"
     CDS             <1..187
                     /locus_tag="VF240_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174289.1"
                     /translation="VRLHLSKCDFCGAELQLLSNHPSVKETPHVPARIPLSLLLLAEH
                     SLPRKHVLKKHPRQNAA"
     gene            454..1605
                     /locus_tag="VF240_03290"
     CDS             454..1605
                     /locus_tag="VF240_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174290.1"
                     /translation="MFGSNVLDVAIGMIFVYLLLSLICSAVNEIIEAWLKKRATDLER
                     GIRELLHDQKGDVWVKDLYDHPLVGGLFRGEYKTEHIGSDGHYQVKGLIGKHGHAHLP
                     SYIPSRNFALALLDLVSKAKDKVAPAADDGKAAAAADGGEEKDRKQESLEKVARAVEA
                     LTKEAGGDAEKARKSVEKWFDSSMDRVSGWYKRRSQVIILFLGFGVTVFANADTIAIS
                     KSLANDPSVRQSLVAASQEYAKSNTAPSPGATPAPTPAPQESTEPASTTAAPTPTPLK
                     ECEKDINSPECRVAKNLHQLEKFGLPIGWDLTNRNAVPTPPWLVAKGDSVAWWTSVGN
                     WLLKLLGWLVTAAAISLGAPFWFDLLNKFMIVRSTVKPHEKSPEEKPIG"
     gene            complement(1667..2092)
                     /locus_tag="VF240_03295"
     CDS             complement(1667..2092)
                     /locus_tag="VF240_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX7174291.1"
                     /translation="MSTPFRDPAIRITLLPRDTNPQGTIFGGIILSYIDMAGAIEAHR
                     HTNVERFVTVAMREVVFHHPVFVGDLVSFYAETTRVGNTSITIRVTVETERHGGATTS
                     GRVKVTEAEVVYVAVDSNRRKMQIDKSAIKGEFGQGETA"
     gene            2496..4676
                     /locus_tag="VF240_03300"
     CDS             2496..4676
                     /locus_tag="VF240_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7174292.1"
                     /translation="MKTLLSRFALLLALSAIAFGANLFAQDNRGARARQRPRPATQRP
                     AATPAPTPSVAPTPARVAVQETLLPPGGRARFDVTHYRIDAELRPAEHLFAAAGDVTF
                     TTLDNTRSVVFELNGSLKVESIERGGVALTNFVQDAVGVDAIGPSVRVDLGEVVAAGT
                     PVTLRFRWGGALVSPEGGPLPSKRLAYVGPEGSYLMYAARWFPFHDYAADRATSDITI
                     SVPAGFEVAGASDEAATAGAESLLRTASPGARRFRFVTRQPALVGNFAAGRYITRSLR
                     MGPYEMQFYVRPGSENFIDTYGQVIGRALEFYTRQYGQPAFGTRFVVAQIDDASLDAY
                     AAHGFQFLSERFFQPARQLSLDDRVERETAFQWWGHSVGLKSFDDVWLSQGLGEWSAY
                     ALRETRLDGARLEAAQRDMMERALMFEQSASILRAPSTLDDQSAAYQAVIYYKGAQVF
                     RMLRETVGKEKFDQVLRRFFTQYKGKNASVDDFERLTTEVVGENMRYFFARWVESTGV
                     PEFAVDYQIIRTRSGKFRARGTVKQNFENLRMPVEITLRAEGDDASTVLPIQDKSEDF
                     DLESTGKPIEVLVDPNYKVLRTSDELRISVVARRGIELFREGSYLEAQRQLEEALKLD
                     KSNSWIYYNLGMLYLEQRNYQQALDNFQAAIDGNLRPSWIAAWARIKRGNAYDAQGDR
                     ARAVHEYNQAVQSGIDFDNAQKAAKAFLETPYDPKATTQQANSR"
     gene            complement(4833..5651)
                     /gene="lpxI"
                     /locus_tag="VF240_03305"
     CDS             complement(4833..5651)
                     /gene="lpxI"
                     /locus_tag="VF240_03305"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX7174293.1"
                     /translation="MRYGLIAGNGKFPFLVVEGARRAGAALAVAAIREETDPAIERAV
                     KDSGGSLTWVGIGQLGRMIRFFKREGVRRAIMAGQVKHVQIFSGALPDVRMLRMLMRL
                     PRRNTDALIGGVAEELAAEGIELIDSTHFLQDHLPAEGVLTRRAPDERERGDIDYGLE
                     VAREIARLDLGQTIVVRARACVAIEAMEGTDAVIRRAGQLAKGRLTVVKVAKPSQDMR
                     FDVPVVGVPTVQAMLEAGATCLSVTAGKTLLFDRAEMIALADRHRLTVVAVNPV"
     gene            complement(5738..6508)
                     /gene="lpxA"
                     /locus_tag="VF240_03310"
     CDS             complement(5738..6508)
                     /gene="lpxA"
                     /locus_tag="VF240_03310"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX7174294.1"
                     /translation="MIHPTAVVSPGAEIADDAQVGPYCIVGGEVRLGARARLDSHCVV
                     EGRTTVGEDTHFFPFVSVGLAPQDLKYRGEPAETRIGRRCQVREFATVHRGTEGGGML
                     TSVGDDCLLMAQAHVAHDCLIGNDVIMANAATLAGHVEVADGANIGAYSGVHQFCRVG
                     REAYVGGYSVVVKDAMPFALTVGNHARCYGLNTVGMRRRGYPQETVDALHHAFRLLLS
                     AKLNTTQALQRVREELAGSAEVEELVRFIETSKRGVVK"
     gene            complement(6600..7052)
                     /gene="fabZ"
                     /locus_tag="VF240_03315"
     CDS             complement(6600..7052)
                     /gene="fabZ"
                     /locus_tag="VF240_03315"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX7174295.1"
                     /translation="MQIPLDAQAIQRILPHRYPFLLVDRIVEFVAGERIVGIKQVTMG
                     DHFFQGHFPGAPVMPGVLIVEALAQVGAVYVLGQMEDRADKLVLFSGIDAARFRRPVV
                     PGDTLRLEVTPIRLGSRVQKMRGVATVDGQPAAEADIMSIIAPRGSLA"
BASE COUNT         1259 a         2326 c         2355 g         1165 t
ORIGIN      
        1 cgtccgcctg cacctctcga agtgcgactt ctgcggcgcc gagttacaac tcctgagcaa
       61 tcatccaagc gtgaaggaga cgccccacgt ccccgcgcgc atcccgctgt cgctgctcct
      121 gctcgccgag cactcgctcc cgcgcaaaca cgtcctgaag aaacacccgc ggcagaacgc
      181 ggcctagaag acacctgcgc ccggcgaact caccggcgcg acggcggccg aacataatcg
      241 ttgacacacg ccggcggcgg gcatatcgtg cgcctgcccg aacgcgcccg ttcttcgccg
      301 gcgctttttc cggcgttcga ttttccagcc ttcataccga gtctcacgcg accgttcaca
      361 gacctgctgt cctgactccc gtcgtcgtcg ccgaaggcgc gaagacgttc gggttctcac
      421 ccggtaaagt cacttgatcg aaaggagtcc cccatgttcg gttccaatgt tctcgatgta
      481 gccatcggga tgatattcgt ctacctcctg ctgagcctca tttgctcggc cgtcaacgag
      541 ataatcgaag cgtggctgaa gaagcgagcg acggaccttg aaaggggcat ccgggaactg
      601 ctccacgacc agaagggcga cgtgtgggtg aaagacctct acgatcatcc gctggtcggc
      661 ggcctgttcc ggggggagta caaaaccgag catatcggaa gcgacggcca ctaccaggtg
      721 aaggggctga tcgggaagca cggccacgcg cacctgccct cttatatccc gtcccgcaac
      781 ttcgccctcg ccctgttgga cctcgtctcc aaggccaagg acaaagtcgc accagcagca
      841 gacgatggga aagccgcggc agcagcagac ggtggggagg agaaagatcg gaagcaggag
      901 agcctcgaga aagtagcgcg ggccgtcgag gcgttgacga aggaggcggg gggcgacgcg
      961 gagaaggcgc ggaagagcgt cgagaaatgg ttcgacagct cgatggacag ggtgtcaggc
     1021 tggtacaagc gccggtcgca ggtgatcatc ctcttcctcg gcttcggcgt gaccgtcttc
     1081 gccaatgccg acacgattgc gatctcgaag agcctcgcca acgacccatc ggtgcgacag
     1141 tcgctcgtcg cggcgtcgca ggagtacgcg aagtccaaca ccgccccgtc gcccggcgcc
     1201 actcccgcgc ccacgcctgc tccgcaggag tctacggagc ctgcgtccac gaccgccgcc
     1261 ccgacgccaa ccccgctgaa ggagtgtgag aaggacatca attcgccgga gtgccgggtg
     1321 gcgaagaacc tgcatcagct tgagaagttc ggcctgccca tcggctggga cctcaccaac
     1381 cggaatgccg tgcccacgcc gccgtggtta gtggctaagg gtgatagtgt ggcctggtgg
     1441 acgagcgtcg gcaactggct gctcaaactt ctgggctggc tggtgacggc cgccgccatc
     1501 tcgctcgggg cgccgttctg gttcgacctc ctgaacaagt tcatgatcgt ccgctcgacc
     1561 gtcaagccgc acgagaagag tcccgaggag aagccgattg gttgagcgcc gggcgcggcg
     1621 gggcggaagg tgtgcgggcg accgaaggcg acgagggcgg cggggcttac gcggtttcgc
     1681 cctgaccgaa ctcgcctttg atcgcactct tatcgatctg catcttgcgg cggttcgagt
     1741 ccacggcgac gtagacgact tcggcctccg tgaccttgac gcgccccgaa gtggtggcgc
     1801 cgccgtggcg ctcggtctcg accgtgacgc ggatggtgat ggaggtgttg ccgacgcgcg
     1861 tcgtctcggc gtagaagctg acgaggtcgc cgacgaagac cgggtggtgg aagacgactt
     1921 cgcgcatggc gacggtgacg aagcgctcga cgttggtatg gcggtgggcc tcgatggccc
     1981 cggccatgtc gatgtagctg aggatgatgc cgccgaagat ggtgccctgc gggttcgtgt
     2041 cgcgcggaag caacgtgatg cggatggccg ggtctctgaa gggtgtgctc atgtgtgtgg
     2101 gtcggcggcg gccgggcgtc tgaacattct cgaacgcggg cgcggcggtt tttttatcgg
     2161 tcgaaagcgt cggtgaggtg aggatggacg gcgtcggcgc gggctgtcaa gcgcggggcc
     2221 gcaaacgttg tcgcgcgagc cccttcggcc ggctccggcg cggtcaatct ttccgccccg
     2281 cgctcgtaca acggcagccc ccgagggcac atcaaaagtc ctgtcgcaaa agaacagacg
     2341 ggcctgccgg tggagttccg cgaagtcgcc cacgccgtcc gcgcgcttca gttcaaagtt
     2401 gcggtaaatt gggcgggggc ctcggcccct tccgaccgcg gatgtaaacg accggccccg
     2461 tactcggcca taaagtttcc gataagaatc cgacgatgaa gacacttctc agccgcttcg
     2521 ctttgctgct cgcgctgtcc gccatagcct tcggcgccaa cctcttcgca caggacaaca
     2581 ggggcgcgcg cgcccgccag aggccgcgcc cggcgacgca gaggccggcg gcgacgcccg
     2641 cgccgacccc gtcggtcgcg ccgacgccgg cgcgcgtcgc cgtgcaggag acgctgctgc
     2701 cgcccggcgg gcgcgcccgc ttcgacgtga cgcactaccg catcgacgcc gaactccgac
     2761 ccgccgagca cctcttcgcc gccgccggcg acgtgacctt cacgacgctc gacaacacgc
     2821 gctcggtcgt cttcgaactc aacggctcgc tcaaggtcga atccatcgaa aggggcggcg
     2881 tcgccctgac gaacttcgtg caggacgccg tgggcgtcga tgccatcggg ccgagcgtgc
     2941 gcgtcgatct cggagaggtc gtcgccgcgg gcacgcccgt cacgctccgc ttccgttggg
     3001 gcggcgcgct cgtcagcccc gagggcgggc cgctgccgag caagcgtctg gcctacgtcg
     3061 gcccagaggg gtcgtacctg atgtacgccg cacgctggtt ccccttccac gactacgcgg
     3121 ccgaccgcgc cacctctgac attactatct ccgtccccgc cggcttcgag gtcgcgggcg
     3181 cgagcgacga ggcggcgacg gccggggccg agtcgctcct gcgcaccgcg tcgccgggcg
     3241 cgcggcgctt ccgcttcgtc acgcgccagc ccgcgctcgt cggtaacttc gcggccggtc
     3301 gctacatcac gcgctcgctc cgcatggggc cttacgagat gcagttctac gtgcgaccgg
     3361 gcagcgaaaa cttcatcgac acctacggtc aggtgatcgg ccgcgccctt gagttctaca
     3421 cgcggcagta cgggcagccg gccttcggca cgcgcttcgt cgtcgcgcag atcgacgacg
     3481 ccagcctcga cgcctacgcc gcgcacggct tccagttcct ctccgagcgt ttcttccaac
     3541 cggcgcgtca gctctcactc gatgatcgcg tcgagcgcga gacggccttc cagtggtggg
     3601 gccattcggt cggcctcaag tcgttcgacg acgtgtggct ctcgcagggc ctcggcgagt
     3661 ggagcgccta cgcgctcagg gagacacggc tcgacggcgc acgcctcgaa gccgcccagc
     3721 gcgacatgat ggagcgggcg ctgatgttcg agcagtcggc ttcgatcctg cgcgcgccct
     3781 cgacgctcga cgaccagtcg gccgcctatc aggccgtcat ctactacaag ggcgcgcagg
     3841 tcttccgcat gctgcgcgag acggtcggca aggaaaagtt cgatcaggtg ctgcgtcgct
     3901 tcttcacgca gtacaagggc aagaacgcca gcgtggacga cttcgagcga ctgacgacgg
     3961 aagtggtcgg cgagaacatg cgctacttct tcgcgcgctg ggtggaatcg acgggcgtgc
     4021 ccgagttcgc cgtcgattac cagatcatcc gcacgcgctc gggcaagttc cgcgcgcgcg
     4081 gcaccgtcaa acagaacttc gagaatttgc ggatgccggt cgagattacg ctccgcgccg
     4141 agggcgacga cgcttcgacc gttctgccca ttcaggacaa gagcgaggac ttcgacctcg
     4201 aatcgacggg caagcccatc gaagtcctcg tcgatccgaa ctacaaggtg ctccgcacgt
     4261 cggacgagtt gcgcatatcg gtcgtcgcgc gtcgcggcat cgagctgttc cgcgaaggct
     4321 cttacctcga agcgcagcgg cagttggaag aagccctcaa gctcgacaag tcgaactcgt
     4381 ggatttacta caatctcggg atgctctatc tggagcagcg caactaccag caggcgctcg
     4441 acaacttcca ggcggccatc gacggcaacc tgcggccctc ctggatcgcc gcctgggcgc
     4501 gcatcaaacg cggcaacgcc tacgacgcgc agggcgaccg cgcccgcgcc gtccacgagt
     4561 acaatcaggc cgtgcaaagc ggcatcgact tcgacaacgc gcagaaggcg gccaaggcgt
     4621 tcctcgaaac cccttacgac ccgaaggcca cgacgcagca agccaacagc agataagcac
     4681 gccgcccgac ccgacagaag aaggcccgcc aacgacgcga gatcgttggc gggccttctt
     4741 gtgagcacgt ctcgcgttga gagtggtgat cacttgagtg aacaggaaat agattcaccg
     4801 cggagacgcg gagggggcgg agttcacgcg gatcagacgg gattgacggc gacgaccgtc
     4861 aggcggtggc ggtcggcgag ggcgatcatc tcggcgcggt cgaagagcag ggtcttgccg
     4921 gcggtgacag agaggcaggt ggcgccggct tcgagcatcg cctgtacggt ggggacgccg
     4981 accaccggca cgtcgaaacg catgtcctgc gagggcttgg cgaccttgac cactgtgagg
     5041 cggcctttcg ccaactgccc ggcgcggcgg atgacggcgt ccgtgccctc catcgcctca
     5101 atcgccacgc acgcgcgcgc gcgcaccacg atggtctgac ccagatcgag ccgcgcgatt
     5161 tcgcgcgcga cttcgagtcc gtagtcgatg tcgccgcgct cgcgctcgtc cggcgcgcgg
     5221 cgcgtgagca cgccctcggc gggcaggtgg tcttgcagga agtgtgtgga gtcgatcagc
     5281 tcgatgccct cggcggccag ctcctcggcg acgccgccga tgagcgcgtc ggtgttgcgc
     5341 cggggcagac gcatgagcat gcggagcatc cgcacgtcgg ggagcgcccc gctgaagatt
     5401 tggacgtgct tgacctgacc cgccatgatc gcccgccgca cgccctcgcg cttgaagaag
     5461 cggatcatcc ggccgagttg cccgatgccg acccacgtca ggctcccccc cgaatccttc
     5521 acggcgcgct cgatggcggg atcggtctcc tccctgatcg ccgccacggc cagcgccgcc
     5581 ccggcgcgcc gcgcgccctc gacgacgaga aagggaaact tcccattgcc tgcgattaag
     5641 ccgtaacgca taagagaaag ttcaaagttc aaagttcaaa gttcaaagtc aacagccgcc
     5701 gcagcgcgct ttgaactttg aactttgaac tttgaactta cttaacgacc ccgcgcttcg
     5761 aggtttcgat gaagcggacg agttcttcga cctcggcgga gccggcgagt tcttcgcgga
     5821 cgcgctggag cgcttgcgtc gtgttgagct tggcggagag gagcaggcgg aaggcgtggt
     5881 ggagcgcatc gaccgtctcc tgcggatagc cgcgccggcg catgccgacc gtgttgaggc
     5941 cgtagcagcg ggcgtggttg ccgaccgtga gcgcaaacgg catcgcgtct ttgacgacga
     6001 ccgagtagcc gccgacgtaa gcctcgcggc cgacgcggca gaactggtgg actcccgagt
     6061 aggccccgat gttcgcgccg tcggcgacct cgacgtggcc ggcgagcgtg gcggcgttcg
     6121 ccatgatgac gtcgttgccg atgagacagt cgtgcgcgac gtgggcctgc gccatgagca
     6181 gacagtcgtc gcccacggag gtgagcatcc cgccgccctc cgtgccgcgg tggacggtcg
     6241 cgaactcgcg cacctgacag cggcgtccga tgcgcgtctc ggccggctcg ccgcggtact
     6301 tcaagtcctg cggggcgagt ccgacggaga cgaagggaaa gaagtgcgtg tcttcgccga
     6361 ccgtggtgcg cccctcgacg acgcagtgtg agtcgagccg cgcgcgcgcg cccaaacgca
     6421 cctcgccgcc gacgatgcag taggggccga cctgcgcgtc gtccgcgatc tccgcgccgg
     6481 ggctgaccac tgcggtgggg tgaatcattt caagtaaaaa ggtaaaaggc aaaaggcaaa
     6541 agtaagaagc acggccacgg gtcttccact tttgcctttt gcctttttac ttttgccttt
     6601 caagcgaggc tgccgcgggg ggcgatgatg ctcatgatgt cggcctcggc ggccggctgc
     6661 ccatcgacgg tcgccacgcc gcgcatcttc tgcacgcggc tccccaggcg aatcggcgtc
     6721 acttccagcc gcagcgtgtc gccggggacg acggggcggc ggaagcgcgc cgcgtcgatg
     6781 ccgctaaaga gcaccagctt gtcggcgcgg tcttccatct gcccgaggac gtagacggcc
     6841 ccgacctgag cgagcgcctc gacgatgagg acgccgggca tgacgggcgc gccggggaag
     6901 tggccctgaa aaaaatggtc gcccatcgta acctgcttga tgccgacgat gcgctcgccc
     6961 gcgacaaact cgacgatgcg gtcaacgagc agaaaggggt agcgatgcgg gaggatgcgc
     7021 tggatggcct gagcgtcgag tgggatctgc ataacaatac cgtaaatggt aaatcgttaa
     7081 tcgtaaatag atttggttcg ggtcg
//
LOCUS       DASVES010000092        13287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_155676, whole genome shotgun sequence.
ACCESSION   DASVES010000092 DASVES010000000
VERSION     DASVES010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155676"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="thiD"
                     /locus_tag="VF240_03320"
     CDS             complement(<1..659)
                     /gene="thiD"
                     /locus_tag="VF240_03320"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX7174296.1"
                     /translation="MPVVLTVAGFDPSGGAGVVADVKTFTAFGCFATAAVTSLTYQNT
                     TGVFGAAHQSAEAVRAQVLPVVEDFRVAAAKTGMLPTREVIREVARLFRETDLPAPVV
                     DPVVRSTSGYDLIDDEALAALRDELLPLARLLTPNIPEAERLCGMRIENRAGMLRAAQ
                     SLRRMGARAVLVKGGHPFAAGGGDAAQEAADLLLEEDERALILSAPRVASTSTHGTGC
                     TL"
     gene            complement(746..1414)
                     /locus_tag="VF240_03325"
     CDS             complement(746..1414)
                     /locus_tag="VF240_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX7174297.1"
                     /translation="MPPELPPAPLTYLVTAGETTPDTTPTSREFAAVLALVERAVVAR
                     VSLVQLREKRLPARVFYELAARAAAITRASGTRLLVNDRADIARAATCDGVHLTTQSL
                     APSAVRRAFGTDLLVGVSAHSLDEARAARDGGDADFCVLGPIFDTPSKVPYGAPLGLG
                     AFRAVARALAPFPVLAIGGVTTENAGTLLRAGAAGVAAIRMFSDSADLRVTVSAVVSA
                     GGRM"
     gene            complement(1404..1769)
                     /locus_tag="VF240_03330"
     CDS             complement(1404..1769)
                     /locus_tag="VF240_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174298.1"
                     /translation="MSSKLTVIFYIILCLEAGVVLTLLPWVRPFGLIGDWGDNYFLVV
                     AAQKAGLQGLQQVVSSGWVRGAVTGLGVLNFLMAFWEMAHFNQTVRAIQTQADAHDRS
                     RAAAEEPAGPTPRPDGDAS"
     gene            complement(1946..4291)
                     /locus_tag="VF240_03335"
     CDS             complement(1946..4291)
                     /locus_tag="VF240_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7174299.1"
                     /translation="MAFDKAKAVRAAEKHLAQGKVPAAVEEYRRIVESDAADFSALNT
                     LGDLYARTGKQQEAMACFRRVAEHYREQGFALKAIAMYKKVTRFVPDDANISVALASL
                     YEQQGLLVEARAQYLTVGDLLARRGNTREALEVLQRVADLDLNNTDIRLRLAEGFERE
                     SLPDLAARAYSEAADRLSARGEYERARQAYTRALSLLPHAHAPLQGLLTVHTALGTAD
                     EAADILEKSVAERPADLELRVMLVRAQVEAEDAAAAERTTDELVARDPSSYPYFFDVA
                     RLALRQKDAERAVRLTGRVIEKALNGREDAQLLELLEDVLRRDPEHVGALLLLARIYE
                     WQRNDSRLRSTLERLVEAAEASGEDEEERRALAQLIRLGHDEPRYRERLLELGGAAEP
                     EQEETAAEDAPAPEASDEVPTFESFIMPDEAGAPASASAAPPSEFNWEATAAPAADAS
                     ADPASSFADLNADFTDDTVAAAAPPSDAAPPPSFTDFQEIDFSAGAPAGGDAMPETGS
                     GTREHLLGQELESVDFYITQGYSDIACETLDMLERQYGRSPLIDERRLRVAAPGVAGE
                     PPPPPVEVEALDDIDTAFAGFTAEAPAAADPAAPAADFYERPPTAAPRASVAAGEGLD
                     PGLAAIFDEFREAVEEAEPLKTEADFENHYNLGLAYMEMDMFDQAVEAFQQANQAVTP
                     NDSTSRYLQCCNMLGHCFMRKGLPKLAAMWFRKGLDAPGHTQDEYQALRYELGTAYEQ
                     MGERDRALDTFTEVYGIDVTYRGVADKLRELQEQRAVNTES"
     gene            4604..6088
                     /locus_tag="VF240_03340"
     CDS             4604..6088
                     /locus_tag="VF240_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7174300.1"
                     /translation="MTSERNGHGAYVGGDAARAPRAARAVALAGFVLYAAAAPHSIAG
                     AWMGLALVVAAWLVRLLATREPGVRPTPFDFPLWLLYAWTVLSAFLSAEPRISIAKLA
                     SVGTFLVFYLTQAMLTRRMAAVAAVVLVASGVAGTLWSLGEIARGRGVVVVEMKDGSP
                     LRQVADLRQGDAIWRVNEGRVSSVEEIDEAIRRTPTGEVVRLSVVARGEHVEWPGFVA
                     TDELKRRASPSGLTGGGPTRRFRASGWTRHYETYAEVLQMVAQLALGLALAFLLRRRR
                     GVTAWLPFVAFVLLGAGIALTAMRTTLAAFAVGAVVVAWRATARGRARVFVVAAVAFA
                     LAFGAVIVWRTRAGGALTLSDPSASLRVQVARVAAGRVPLHPVFGHGMDAVQRHWQEW
                     GFPGTDMLHTHSTPLQLAFDRGLPAVLFWLWLLAAFWLAATRAERLWRDTDDAATHGL
                     ALGTTGALAGFAASSLVNYNFGDSEVALLLWWLMGAIVVLSREQ"
     gene            complement(6169..6993)
                     /locus_tag="VF240_03345"
     CDS             complement(6169..6993)
                     /locus_tag="VF240_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7174301.1"
                     /translation="MISAQAARRAVWRPLWELPGRFDLVWPLARRFTAARYRGSALGL
                     LWAVVTPLVMIATFTFIFAGIFGARFGREGTAVDYALYLFCGLLPWTAFQESVQGSSG
                     VVVAHANLVKRVVFPLETLPVAQVLAAVAGQLAGTAVLLLAALAVRGELHATVLWLPV
                     LLLPQLVLTLGASWLVASLGVFVRDTAQAAGLLLTAWMYLTPIIYPESAVPADYRRWI
                     ELNPFTPLVRSYRRVLLDGAAPDWPGLAYFSLTALAIFFFGYWWFARTRKNFADVV"
     gene            complement(7077..7286)
                     /locus_tag="VF240_03350"
     CDS             complement(7077..7286)
                     /locus_tag="VF240_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174302.1"
                     /translation="MPRAGKKKAAAPPKNPRRRGAPDPESVVEKIPFTSPSGETFVIL
                     RTDEVDAYEEPALKKKKARRGRKRG"
     gene            complement(7321..8172)
                     /locus_tag="VF240_03355"
     CDS             complement(7321..8172)
                     /locus_tag="VF240_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174303.1"
                     /translation="MPTQKKMQIAYPPAVIAALAAVSKAAPETTTMAMASAAAARATP
                     ADESVLMRLQFKPPSGAPEFTILRTVEVDKYEPPALAALTTPHGVMALAASSGDNFTG
                     TDRRVAKISIADAAMEEFADLKDLIATLPSTSFMKNHKPKITRDKNMNRVEVEKRNVR
                     VHAFLYAASRENDNDFHLIIGRDPSAGEEMYMNVELSGLPPASSKSFTKLKTARNAYK
                     KYWGSELPGTSYDFYTNAPRPVLIEGSLFFDVTHATGQKPGPQKLSKKIPTIWEIHPI
                     TRIKFKA"
     gene            8512..9801
                     /locus_tag="VF240_03360"
     CDS             8512..9801
                     /locus_tag="VF240_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7174304.1"
                     /translation="MAVALSVEDVSKQYRIYERPGDRLTESLTRGRLKRHREFWALRD
                     VSFAVGRGETVGLVGPNGCGKSTLLQIIAGTLEPTHGSVQTEGRVAALLELGAGFNPE
                     FTGRENALMNAALLGLQRREAERLLPEVERFAEIGAFFEQSVKTYSSGMYVRLAFAIA
                     ASVEPDILVIDEALAVGDAVFQHRCLRRIKEFQERGATVLLVSHDAAAVRALCTRAVL
                     LHSGRLLADGRPSDVLNRYQKIIMEREEAFEASRPASVSGDGDGGDAPEDEKPPSLSY
                     TYRHGDGSAQVEGAQLLDATRRAVAVVDSGEGLTARVRVRFREETEEPVVGFLIRNRH
                     GIHVYGTNTKEQAVTLGRFAAGETLEVNFAFDCWLGIDDYSVSFAVHSRDGVAYDWLD
                     GVVFFRVTSAVLVEGVANLNATATARRVELRGAAGKA"
     gene            9917..10705
                     /locus_tag="VF240_03365"
     CDS             9917..10705
                     /locus_tag="VF240_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174305.1"
                     /translation="MNQSSIMVDLEVEKTLREIRERVRAEAAAGGEEPAQAPTRGAEL
                     STLDAATGAAGVSSALGRLEVNVSTAERARSRLPPLVSNRKGWPARLELWVKRQIKRA
                     THWFTWEQVNYNAAVYHALRDTHAALAALDSELASLRRQLHTFEEQLDARDARLSEEL
                     AAGVASLRGEQRAFVDDIHREQRALASDLRREQGALTDGLRGDINGGRVEARERAAHV
                     LEEQRVLFRQLSLEASERAVACERSRRDLETRLAEIKKDVMSDK"
     gene            10764..12095
                     /locus_tag="VF240_03370"
     CDS             10764..12095
                     /locus_tag="VF240_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7174306.1"
                     /translation="MKDLYARAGIEDVAEFYRTKFVSDEVLDARYFRPVERFHMHLAR
                     TMWVYDNVRAGSAVLDVGCGAGTLALLKRKGVALAGVDLSADCARAARANGYDATARA
                     DLRRLPFPDQSFDYVASLDVLGHVSFDEKSAVISEIRRVLRPGGVTLHGIECTDRGMQ
                     KSYEEMTEQELRHFIQIDGHVGLEEEEEVAARFRRVFPHVAWEPRYALSLSSEEFIKQ
                     HDEYGLPFEEDFVEYLRGLSFKERRAFDMAMGYVFAKVSDLNIRLPRSGLYMFLKASD
                     APLGPFNNEHRDRRGLFSGAPREEDAPRFCLDRDAGARFDDGWHEPNVLPPVARWMGA
                     RARVSFSAARVSELRLDLTTHLPELGPARPLGLEILLNGARLSAFSLFRYGWLELAFP
                     VPEDVNAAAGGRFELEIRADRTWQPRPGDAEGRDDREMSVAVCNIEVISDE"
     gene            12222..13274
                     /locus_tag="VF240_03375"
     CDS             12222..13274
                     /locus_tag="VF240_03375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7174307.1"
                     /translation="MIRNVLICTTQVPFTTGGAESHIENLRRALVAHGYNAETVALPF
                     KWYPPGEIMRGALAWRLLDLSESNGKPVDLVLGMKFPAYVVAHPRKVLWILHQHRSAY
                     NLAGTPFDDLSTYPEGPRVREWIRDCDTRFIPEARRVFANSRTVAERLRRYNRIESEP
                     LYHPPPRAERLRPGAQGDYVFYPSRLEPQKRQEMLIEAMRYVRAPLRAVFAGGSRDAA
                     HYEQLARAHGVSERVSFRGFVTEDEMIDLYAGALAVCYLPFDEDYGYVTLEAMLAAKP
                     VVVARDGGGAAELVEDGATGLVADPDPRALAESLNSLHADRSRALRMGERGREKLLAM
                     NLSWDNVVERLISAAE"
BASE COUNT         2064 a         4436 c         4599 g         2188 t
ORIGIN      
        1 agcgtgcagc ccgttccgtg cgtcgaggtg gaggcgacgc gcggcgcgct gagaatgagc
       61 gcgcgctcgt cctcttcgag cagaaggtct gcggcctctt gggcggcgtc gccgccgccc
      121 gcggcgaacg ggtggccgcc cttgacgagc acggcgcgcg cgcccatgcg tcggagcgac
      181 tgtgcggcgc ggagcatccc cgcgcgattt tcaatccgca tcccgcacag gcgctcggcc
      241 tcggggatgt tgggcgtcag caggcgcgca agaggcagga gttcatctct gagcgcggcg
      301 agcgcctcgt cgtcgatcag gtcatacccg gaggtggagc gcacgacggg gtcaacgacc
      361 ggcgcgggca aatccgtctc gcggaacaga cgcgcgacct cgcggatgac ctcgcgcgtc
      421 gggagcatcc cggtcttggc ggcggccacg cggaagtctt cgacgacggg caagacctgt
      481 gcgcgcacgg cctcggccga ctggtgcgcc gcgccgaaga cgcccgtcgt gttctggtag
      541 gtcaaggacg taacggccgc ggtggcgaag cagccgaagg cggtgaacgt cttcacgtcg
      601 gcgacgacgc cggcgccgcc cgacgggtcg aagccggcga cggtcagcac aaccggcatc
      661 ctcgttgtca actgctcacc catgccgcgt ttaatttatc cgagcgagac gcggaacaca
      721 aagacccgcc gccgcgccct acacactaca tgcgcccgcc ggcactcaca acggccgaga
      781 ccgtcacgcg caaatccgcc gagtcggaga acatgcggat cgcggcgacg ccggccgcgc
      841 ccgcgcgcag cagcgtgccc gcgttctccg tcgtgacgcc gccgatggcg aggacgggga
      901 agggcgcgag cgcacgagcc acagcgcgaa aggcgccgag gccgaggggc gctccgtaag
      961 gaactttcga gggcgtgtcg aagatggggc cgaggacgca gaagtcggcg tcgccgccat
     1021 ctcgcgccgc gcgcgcctca tcgagcgagt gcgcggagac gccgactaag aggtctgtgc
     1081 cgaaggcgcg gcgcacggcc gaaggcgcga gtgattgtgt ggtgaggtgg acgccgtcgc
     1141 acgtggcggc gcgcgcgatg tcggcgcggt cgttgacgag caggcgcgtg ccgctcgcgc
     1201 gtgtgatcgc ggcggcgcgc gcggcaagtt cgtagaagac gcgcgccggc agacgcttct
     1261 cgcgcagttg gacgagcgag acgcgggcga cgacagcgcg ttcgacgagc gcgaggacgg
     1321 cggcgaactc tcttgaagtg ggggtggtgt cgggcgtcgt ctcgccggcc gtgacgaggt
     1381 aggtcagggg cgctggggga agttcaggag gcatcgccgt cggggcgcgg tgtcggcccg
     1441 gccggctctt cagcggcggc gcggctgcgg tcgtgcgcgt cggcctgagt ctggatggcg
     1501 cgcaccgtct ggttgaagtg ggccatctcc cagaaggcca tcaggaagtt gagcacgccg
     1561 aggcccgtca ccgcgccgcg tacccagccc gacgagacga cctgctgcaa gccctggagg
     1621 ccagccttct gcgccgcgac gactagaaaa tagttgtcgc cccagtcgcc gatcagcccg
     1681 aaggggcgca cccacggcag cagcgtcagc acgacgcccg cctccaggca caggatgatg
     1741 taaaagatga cggtgagctt ggaagacatg gaaagacgtg aatcgtaaat ggtaaatcgt
     1801 caatcgtggt tgagacggcc gctggcttga atggtaaatc gcgagtcggg agaaagcaaa
     1861 cctgcccgtt cacgatttac gcttcacgag ccgccattcg cgcttcacga acggtgttga
     1921 cgattgacga ttgacgattg acgatttacg actcggtgtt gacggccctt tgctcctgaa
     1981 gttcgcggag cttgtcggcg acgccgcggt aggtaacgtc gatgccgtag acctctgtga
     2041 aggtgtcgag ggcgcggtcg cgctcgccca tctgctcgta ggcggtgccg agttcgtagc
     2101 gtagcgcttg gtactcatcc tgtgtgtggc cgggggcgtc gagtcccttg cggaaccaca
     2161 tggcggctaa tttgggcagc cccttccgca tgaagcaatg gccgagcatg ttgcagcact
     2221 ggagatagcg cgaggtcgag tcgttcggcg tgaccgcctg attggcctgc tggaaggcct
     2281 cgaccgcctg atcgaacatg tccatctcca tgtaagccag gccgaggttg tagtggttct
     2341 cgaagtcggc ctcggtcttc agcggctccg cctcctcgac ggcttcgcgg aactcgtcga
     2401 agatggcggc cagccccgga tcgagtccct cgcccgcggc gacactcgcg cgcggcgcgg
     2461 ccgttggcgg ccgttcatag aagtccgcgg ccggcgcggc ggggtcggcg gcggccggag
     2521 cctcggccgt gaagccggcg aaggccgtgt cgatgtcgtc gagcgcctcg acttcgacgg
     2581 gtggcggcgg cggctccccg gcgacgccgg gcgcggcgac gcgaagtcgg cgctcgtcga
     2641 tcagggggct tcggccgtac tggcgttcga gcatgtcgag ggtttcacac gcgatgtcgg
     2701 agtagccctg cgtgatgtag aaatcgacgc tctcaagctc ctgcccgagc aggtgctcgc
     2761 gcgtccccga gccggtctcg ggcatggcgt cgccgccagc gggcgcgccc gcgctgaagt
     2821 cgatctcctg aaagtcggtg aacgagggcg ggggcgcggc gtcagacggc ggcgcggcgg
     2881 cggcgacggt gtcgtccgtg aaatccgcgt tgaggtcggc gaacgacgag gcggggtcgg
     2941 ccgacgcgtc ggcggcgggg gcggccgtcg cttcccagtt gaactcggac ggcggcgcgg
     3001 cggacgccga cgcaggcgcg cccgcttcgt ccggcatgat gaagctctcg aaggtcggca
     3061 cctcgtccga cgcctccggc gcgggtgcgt cttcggcggc cgtctcttcc tgctccggct
     3121 cggccgcgcc gccgagttcg agcaggcgct cgcggtagcg cggctcgtcg tggccgagcc
     3181 ggatgagttg cgccagcgcc cggcgctcct cctcgtcctc gcccgaagcc tcggccgctt
     3241 cgacgagccg ctccagcgtc gagcgcagac gcgagtcgtt gcgctgccac tcgtagatgc
     3301 gcgcgagcag gagcagcgcc ccgacgtgtt cggggtcgcg ccgcaacaca tcttcgagca
     3361 attcgaggag ctgggcgtcc tcgcgtccgt tcagcgcctt ttcgatgacg cggcccgtga
     3421 gccggacggc gcgctcggcg tccttctgtc gcagggcgag gcgggccacg tcgaagaagt
     3481 agggatagct actcgggtcg cgggccacga gttcgtcggt ggtgcgctcg gcggcggcgg
     3541 cgtcctcggc ctcgacctgt gcgcgcacga gcatcacacg cagctccaga tcggcgggcc
     3601 gctcggcgac cgacttctcc aggatgtcgg cggcctcgtc ggccgtgcca agcgcggtgt
     3661 ggacggtgag cagcccctgg agcggggcgt gtgcgtgcgg gagcagggag agggcgcgcg
     3721 tgtaggcctg gcgggcgcgc tcatattcgc cgcgcgcgga gaggcggtcg gcggcctcgc
     3781 tgtaggcccg cgcggcgagg tcgggcagcg attcgcgctc gaagccctcg gcgaggcgca
     3841 ggcggatgtc ggtgttgttg aggtcgaggt cggcgacgcg ctggaggact tcgagcgcct
     3901 cgcgcgtgtt gccgcggcgt gcgaggaggt cgcccaccgt gagatactgc gcgcgcgctt
     3961 cgacgaggag tccttgttgt tcgtagagcg aggcgagggc gaccgagatg ttggcgtcgt
     4021 cgggaacgaa gcgcgtcacc ttcttgtaca tcgcgatggc cttgagggcg aagccctgct
     4081 cgcgatagtg ctcggccacg cggcggaagc aggccatggc ttcctgctgc ttgcccgtgc
     4141 gcgcgtagag gtcgccgagc gtgttgagcg cggagaagtc tgcggcgtcg ctctcgacga
     4201 tgcgccgata ctcttcgacg gcggccggca ccttaccctg ggcaagatgc ttctctgccg
     4261 cacgcacagc ctttgccttg tcgaatgcca ttgggaatga ttgctctgtg gggacggggc
     4321 ttcagataga gacgagggct gaggtcggca gagtgactcg cgcccggctg aaaagcgtct
     4381 cgcctggctg cgccctgtcc cggcgtcagg caatttggta cgctatcacg cgctcgcggc
     4441 gagtgtcaac ctgatagttt tccaatagtt tacgagcggc gcgatgaggg atgagggatg
     4501 agggatgagg gatgaaaagg gcgttggcgg acggccttcg acccatctcc tctggctcca
     4561 acgcgctccc gcaagcggaa cttcggaaca taaaaattcg cggatgactt ctgaacgaaa
     4621 cggccacggc gcgtatgtcg gcggggatgc ggcgcgcgcc ccgcgcgccg cgcgtgccgt
     4681 ggccctcgcc ggcttcgtgc tctacgcggc ggcggcgccg cactcgattg cgggcgcgtg
     4741 gatggggctc gcgctcgtcg tcgcggcgtg gctcgtgcgg ctgctcgcca cgcgcgagcc
     4801 gggcgtgcgg cccacgccgt tcgactttcc gctctggctc ctctacgcgt ggacggttct
     4861 ttcggcgttc ctctccgccg agccgcgcat cagcatcgcg aagctcgcct cggtcggcac
     4921 cttcctcgtc ttctatctca cgcaggcgat gctcacgcgc cgcatggcgg cggtcgcggc
     4981 ggtcgtgctc gtcgcctcgg gtgtagcggg cacgctgtgg agtctgggtg agatcgctcg
     5041 tgggcgcggc gtcgtcgtcg tggagatgaa agacggaagc cccttgcggc aggtggccga
     5101 cctccggcag ggcgacgcga tctggcgtgt gaacgagggg cgcgtgtcgt cggtcgagga
     5161 gatcgacgag gcgatcaggc ggacgccgac gggcgaggtt gtgcgtctaa gcgtcgtggc
     5221 gcgcggggag cacgtcgagt ggccgggctt cgtggcgacg gacgagttga agcggcgcgc
     5281 ctcgccctcg ggtctcacag gcggcgggcc gacgcgacgc ttccgcgcct cgggctggac
     5341 gcgccactac gagacctacg ccgaggtgct tcagatggtg gcgcagctcg cgctcggtct
     5401 ggcgctcgcg tttctcttgc ggcgacggcg cggcgtgacc gcgtggctgc cgttcgtggc
     5461 cttcgtgctg ctcggcgcag ggattgcgct gacggcgatg cggacgacgc tcgcggcctt
     5521 cgccgtgggc gcagtggtcg tcgcgtggag ggcgacggcg cggggccgcg cacgcgtgtt
     5581 cgtcgtggcg gcggtcgctt tcgcgctggc gttcggcgcg gtcatcgtgt ggcgcacgcg
     5641 cgcgggcggc gcgctcacct tgagcgaccc gagcgcgagt ctgcgcgtgc aggtggcgcg
     5701 cgtggcggcg ggccgcgtcc ccctgcaccc cgtcttcggt cacggcatgg acgccgtgca
     5761 gagacactgg caggagtggg gcttccccgg cacggacatg ctccataccc attcgacgcc
     5821 gctgcaactc gccttcgacc gcggcctccc cgccgtcctc ttctggctct ggctcctcgc
     5881 cgccttctgg ctcgccgcca cgcgcgccga acgcctctgg cgcgacaccg acgacgccgc
     5941 cacccacggc ctcgccctcg gcacgaccgg cgccctcgcc ggcttcgccg ccagctccct
     6001 cgtcaactac aacttcggcg actccgaggt cgcgctcctc ctctggtggc tgatgggagc
     6061 aatcgtcgtg ttgagccgag agcaatagga gtcaggagtc agaagtcaga atgaagagag
     6121 cgacatgtgt tcattctgac ttctgactcc tgactcctga ctcctgtttt aaaccacgtc
     6181 ggcgaagttc ttgcgcgtcc gcgcgaacca ccagtaaccg aagaagaaga tggcgagggc
     6241 ggtgagggag aagtatgcga ggccgggcca gtcgggggcg gcgccgtcga ggagcacgcg
     6301 gcggtagctg cgcaccagcg gcgtgaaggg gttgagttcg atccagcggc ggtagtcggc
     6361 cgggacagcc gactccgggt agatgatcgg cgtcaggtac atccacgccg tcagcagcag
     6421 cccggcggcc tgcgccgtgt cgcgcacaaa gacgccgagc gaggcgacga gccacgacgc
     6481 gccgagcgtc agcacgagct gcggcaggag cagcacgggg agccacagca ccgtggcgtg
     6541 caactcgccg cgcacagcga gcgcggcaag gagaagcacc gccgtccccg ccaactgccc
     6601 cgccacggcc gcgagcacct gcgccaccgg cagtgtctcc agcggaaaga ccacgcgctt
     6661 gacgaggttg gcgtgcgcga cgacgacgcc cgaagacccc tgcaccgact cctggaaggc
     6721 cgtccacggc aaaagcccgc agaagaggta gagcgcgtaa tcgacggccg tcccctcacg
     6781 gccgaagcgc gcgccgaaga tgccggcgaa gatgaacgtg aaggtggcga tcatcacgag
     6841 cggcgtgacg acggcccaga gcagccccaa cgccgagccg cggtagcgcg ccgccgtgaa
     6901 gcgccgcgcc agcggccaga cgaggtcaaa cctgccgggc aactcccaca gcggccgcca
     6961 caccgcccgc ctcgccgcct gcgccgagat catgccgcgc tccttcgctt cgtccgaacc
     7021 gggaacgtct cgactgtggc ggccgcagtc gatttatgca cgcgcgcgca cacagctcaa
     7081 ccgcgcttcc gcccgcgccg ggccttcttc ttcttcaacg ccggctcttc gtaagcgtcc
     7141 acctcgtccg tccgcaggat gacgaacgtc tcgccgctcg gcgacgtgaa ggggattttt
     7201 tcgacgaccg actcggggtc gggcgccccg cgccggcgcg ggtttttcgg cggggcggca
     7261 gctttcttct tacccgcacg cggcatgtgc gttaacctcc ttcggcggcg ggccgcagcg
     7321 ctacgccttg aatttgatcc tcgtgatcgg gtgtatctcc cagatcgtcg gaatcttctt
     7381 ggacagcttc tgcgggccgg gcttctggcc ggtagcgtgg gtcacatcga agaacaacga
     7441 gccttcgatg aggacggggc ggggcgcatt ggtgtagaag tcatatgagg tgcccggcag
     7501 ttcactgccc cagtatttct tgtacgcgtt acgggcggtc ttcaacttcg tgaaggattt
     7561 actggaggcc ggcggcaggc cggagagttc cacgttcatg tacatctcct cgccggcgct
     7621 cgggtcgcgg ccgatgatga ggtggaagtc gttgtcgttc tcgcggcttg cggcgtacag
     7681 gaaggcgtgg acgcgcacgt tccgcttctc gacctcgacg cggttcatat tcttgtctct
     7741 cgtgatcttc ggcttgtgat tcttcatgaa gctcgtggag ggcagcgtgg cgatcaagtc
     7801 cttcaggtcg gcgaactctt ccatcgccgc gtcggcgatg gagattttcg cgaccctccg
     7861 gtcagtcccg gtgaaattgt ccccgctgct cgcggcgagc gccatgactc cgtgcggagt
     7921 ggtcagcgcc gccaacgccg gcggctcgta tttgtccact tcgaccgtcc gcaggatggt
     7981 gaattcgggc gccccgctcg gcggcttgaa ctgtagccgc atgaggacgg actcgtcggc
     8041 cggcgtggcg cgggccgccg ccgccgaggc catggccatg gtcgtcgttt cgggagcggc
     8101 cttgctgacg gcggccaggg cggcgatcac ggcggggggg taggctatct gcatcttttt
     8161 ctgagtcggc atgtttactc tccatttcgc ggactgagta gtaggtggtt gccgcctgtg
     8221 aggcggaagt ttaaaggccg gtcgggaacc cgagaactgg aagggttcgg ccacgcgctc
     8281 acggccggcg gcgctccaat gccgaagagt gatgaccgcc ggcctgttcg ggatcgccgc
     8341 ccatagtaga caaagggcag acattattca acgaatttga gaggacggcc gtgccacgtg
     8401 tggccgcggc ccgcgcgcgc ggggacttca gcgcgcgacc tgaacccgac tcccgcccgc
     8461 gcgcacgcgc tgtctggctt ccgtcttccg tgttctgtta cattcggccg catggccgtc
     8521 gccctcagcg tcgaggacgt gtcgaagcag taccgcatct acgagcggcc gggcgaccgg
     8581 ctgaccgagt cgctgacgcg tgggcgcttg aagcgacatc gcgagttctg ggcgctcaga
     8641 gacgtgagtt tcgcggtcgg gcggggcgag acggtcggcc tcgtcgggcc gaacgggtgc
     8701 ggcaaatcca cgctcctcca gatcatcgcg ggcacacttg agccgacgca cgggagcgtg
     8761 caaaccgaag ggcgcgtcgc cgcgctgctc gaactcggcg ccggcttcaa ccccgagttc
     8821 accgggcgcg agaacgccct gatgaacgcc gcgctcctcg gccttcagcg gcgcgaggcc
     8881 gagcggctgc tccccgaggt cgagcgcttc gccgagatcg gcgcgttctt cgagcagagc
     8941 gtcaagacct actcgtcggg gatgtacgtc cgcctcgcct tcgccatcgc cgcctcggtc
     9001 gagcctgaca tcctcgtcat cgacgaggcg ctggccgtcg gcgacgccgt cttccagcat
     9061 cgttgcctgc gccgcatcaa ggagtttcag gagcgcggcg cgaccgtgct gctcgtctcg
     9121 cacgacgcgg cggccgtgcg cgccctctgt acgcgggccg tcctccttca ctcggggcgc
     9181 ctcctcgccg acggccgccc ctcggacgtg ctcaaccgct atcagaagat catcatggag
     9241 cgcgaggagg ctttcgaggc ttcgcgcccc gcgagcgtga gcggcgacgg tgacggaggc
     9301 gatgctcctg aagatgaaaa gccaccgtcc ctcagctaca cttaccgaca cggcgacggg
     9361 agcgcgcagg tcgagggcgc gcagctcctc gacgcgacgc ggcgcgcagt cgccgtggtc
     9421 gattcgggcg aggggctgac ggcgcgcgtg cgcgtccgtt tccgcgaaga gacggaggag
     9481 ccggtggtcg gctttctcat ccgcaaccgc cacggcatcc acgtctacgg gacgaacacg
     9541 aaggagcagg ccgtcacgct cgggcgcttc gcggcgggcg agacgctgga ggtcaacttc
     9601 gccttcgact gctggctcgg gatagacgac tactccgtct ccttcgccgt ccacagccgc
     9661 gacggcgtgg cttacgactg gctcgacggc gttgtctttt ttcgcgtcac cagcgccgtc
     9721 ctcgtcgaag gcgtcgccaa cctgaacgcg acggcgaccg cccgccgcgt cgaactgcgc
     9781 ggcgcggcgg ggaaagcatg agtacagtac cgcgagcggt agcgagcggg tctagttgct
     9841 cggtgcgggc cactcatcgc agttgcaccc gcgaggcttc cgctcgcggt actgctctca
     9901 ttgagccgat gagtcaatga atcaatcttc catcatggtt gacctcgaag tcgaaaagac
     9961 cctgcgtgag atacgcgagc gcgtgcgggc cgaggccgcg gcgggcggcg aggaaccggc
    10021 tcaggccccc acacgcggcg cggagctttc gacgctcgac gccgcaacgg gcgcggccgg
    10081 ggtgtcgagc gcactcgggc gtctcgaagt gaacgtgtcc acggccgagc gcgcgcgctc
    10141 ccggcttccg ccgctcgtca gcaaccggaa gggctggccc gcgcggctcg aactctgggt
    10201 caagcggcag atcaagcggg ccacccactg gttcacgtgg gaacaggtca actacaacgc
    10261 ggccgtctat cacgccctgc gcgacacgca cgcggccctc gccgcgctcg actcggaact
    10321 cgcctcgctg cgccggcaac ttcacacctt tgaagaacag ctcgacgccc gcgacgcccg
    10381 cctctcggaa gagctggcgg ccggcgtcgc ctcgctgcgc ggcgaacagc gtgccttcgt
    10441 cgatgacatt caccgcgagc agcgcgcgct cgcctccgac ctgcgccgcg agcagggcgc
    10501 gctcaccgac ggcctgcgcg gcgacataaa cggggggcgg gtcgaagcgc gcgagcgcgc
    10561 cgcgcacgtc ctcgaagagc agcgcgtgtt gttccgccag ctctcgctcg aagccagcga
    10621 gcgagccgtc gcttgcgaac gctcgcgccg cgatctggag acgcgtctcg cagaaatcaa
    10681 gaaagacgtg atgagtgata agtgatgagt gataagaaaa ccctttctta ctcttatcac
    10741 tcatcactta tcactcatca cttatgaaag acctgtacgc gcgagccggc atcgaggacg
    10801 tggcggagtt ctaccgcacg aagttcgtct ccgacgaggt tctggacgcg cgctactttc
    10861 gtcccgttga acgcttccac atgcatctgg cgcgcacgat gtgggtctac gacaacgtgc
    10921 gcgccggctc cgccgtgctc gacgtgggtt gcggcgccgg cacgctcgcg ctcctcaagc
    10981 gcaagggcgt ggcgctggcc ggcgtcgatc tctcggccga ctgcgcgcgc gccgcccgcg
    11041 ccaacggcta cgacgccacc gcccgcgcag acctgcgccg cctccccttc cccgaccaat
    11101 ccttcgacta cgtcgccagc ctcgacgtgc tcggccacgt ctccttcgac gagaagtcgg
    11161 cggtcatctc cgaaatccgt cgcgtgctcc gccccggcgg cgtcaccctg cacggcatcg
    11221 agtgtaccga ccgcggcatg cagaaatctt acgaggagat gaccgagcag gagctgcgcc
    11281 acttcataca aatcgacggc cacgtcggac ttgaggaaga agaggaggtg gccgcgcgct
    11341 tccgccgcgt gttcccgcac gtcgcgtggg agccgcgcta cgccctgtcg ttgagcagcg
    11401 aggaattcat caagcagcac gacgagtatg gcctgccatt cgaggaggac ttcgtcgaat
    11461 acctgcgcgg cctctccttc aaggagcggc gcgctttcga catggcgatg ggctatgtct
    11521 tcgccaaggt ctctgatctc aacatccggc tcccgcgcag cggcctctac atgttcctca
    11581 aggcgtcgga cgcgccgctc ggccccttca acaacgagca ccgcgaccgc cgcggcctct
    11641 tcagcggcgc gccgcgcgag gaggacgcgc cgcgtttctg cctcgaccgc gacgcgggcg
    11701 cgcgcttcga cgacggctgg cacgagccga acgtgctgcc gcccgtcgca cgctggatgg
    11761 gcgcgcgcgc gcgtgtgagc ttcagcgcag cgcgcgtctc ggagttgcgc ctcgacctga
    11821 cgacgcatct gcccgaactc ggccccgcgc gcccgctcgg cctcgaaatt cttctcaacg
    11881 gcgcgcgtct ttcggccttt tcgctcttcc gctatggctg gctggagctt gcgttccctg
    11941 tcccggaaga cgtgaacgcc gcggccggcg gtcgcttcga gctggagata cgcgctgacc
    12001 gcacgtggca gccgcgaccc ggcgacgccg aagggcgcga cgaccgggag atgagcgtcg
    12061 ccgtctgcaa catagaggtg ataagtgatg agtgataagt gataagagat gttagctgtt
    12121 ggcgattagc tcttagccaa acagctaaca gctaatcgct aatagctcct tcttatcact
    12181 catcacttat cacttatcac tcatcactca tcactcctcg aatgattcgc aacgtcctca
    12241 tctgcaccac gcaagtgccc ttcaccacgg ggggcgcgga gtcgcacatc gagaacctgc
    12301 gccgcgcgct cgtcgcacac ggctacaacg ccgagacggt cgcgctcccc ttcaagtggt
    12361 atccgcccgg agagatcatg cgcggcgcgc tcgcctggcg cctgctcgat ttgtcggagt
    12421 cgaacggcaa gccagtagat ttagtgttgg gcatgaagtt ccccgcctac gtcgtcgcgc
    12481 acccgcgcaa ggtgctgtgg atactgcacc agcaccgctc ggcctataac ctcgccggca
    12541 ctcccttcga cgacctctcg acctaccccg aggggccgcg cgtgcgcgag tggatacgcg
    12601 actgcgacac gcgcttcatc cccgaggcgc gccgcgtgtt cgccaactcc cgcaccgtcg
    12661 ccgaacgcct gcgtcgctac aaccgcatcg agagcgagcc gctttaccac ccgccgccgc
    12721 gcgccgaacg cctgcgcccc ggcgcgcagg gtgactacgt cttctacccg agccgtctgg
    12781 agccgcagaa gcgacaggag atgctcatcg aggcgatgcg ctacgtccgc gcgccgctgc
    12841 gcgccgtctt cgccggcggc tcgcgcgacg ccgcacacta cgaacaactc gcgcgcgcgc
    12901 acggcgtctc cgaacgcgtc tccttccgcg gcttcgtcac ggaggacgag atgatcgacc
    12961 tctacgccgg cgcgctcgcc gtctgctacc tgccctttga tgaagactac ggctacgtga
    13021 cgctcgaagc gatgctcgcg gccaagcccg tcgtcgtcgc gcgcgacggc gggggcgcgg
    13081 ccgaactcgt cgaagacggc gcgaccggtc tcgtcgccga ccccgatcca cgcgcgcttg
    13141 ccgaatccct gaactcgctc cacgccgacc gcagccgcgc cctccgcatg ggtgagcgcg
    13201 gccgcgagaa actgctcgcg atgaatctct cctgggacaa cgtggtcgaa cgcctcatct
    13261 ccgcggcgga gtaaggcaaa agtaaaa
//
LOCUS       DASVES010000093         4564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_397176, whole genome shotgun sequence.
ACCESSION   DASVES010000093 DASVES010000000
VERSION     DASVES010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397176"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="VF240_03380"
     CDS             complement(<1..1667)
                     /locus_tag="VF240_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7174308.1"
                     /translation="MKKFFAFLALAVTCALALPFGLASAQRSGLNPSHRAADAAAANN
                     NSADERFIIKFRQFGKFASDDVRARGGQVVRELPELASVAARLPEHARVALQNNPNVE
                     FIEVDPRRYPMQTGAPWSDLPIAGTNETTPYGIQMVQADQVSDSLAANRKVCIIDSGY
                     KDAHEDLMDRTSGHVTASTNAGTGDPYRDYDSHGTHVAGTIAGLRNGTGVVGVLPSGT
                     VNLHIVKVFGDNGAWAYSSDLAAAVQQCRDAGSNVVSMSLGGLGGSVTEQNAFQNAYN
                     AGVLSIAAAGNDGNTATNYPAGYSSVVSVAAVDNAEAHATFSNVNADVEIAAPGVATL
                     SSIAYKDTQTLGVNGTTYTGHHVEYAALSTPSGVTATLVGGGLCDTTGGGAWTGKVVL
                     CERGTISFYDKVHNVQLGGGAAAVIYNNLAEPLFATLGEGNSSTIPAIGLSQADGQAA
                     LAQAGSVGTVSSYEDQTFNNVYDYFNGTSMATPHVSAVAALVWSYNPSWTNAQIRNAL
                     TATARDKGTAGRDTTFGYGIVQAKAALDYLSAPPAGPAAPSGLVVTAT"
     gene            complement(2037..3605)
                     /locus_tag="VF240_03385"
     CDS             complement(2037..3605)
                     /locus_tag="VF240_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174309.1"
                     /translation="MKKVLTSLFTLCAVLLLPTLVFGQGRIDKLPAAKAVKHDTSRPL
                     REITPVKGGASRTDFEPKRPAEPGGEFNDPVVQSSAVPLVAITAGLNFEGVGEGLPGY
                     NANVAPPDTTGDVGATQYVQWVNSHFAVFDKVSGAMTYGPAAGNTLWAGFGGACQTRN
                     DGDPIVQYDQIANRWVMTQFAVPGGAAGYWQCVAVSTTSDATGSYNRYAFQYDQFPDY
                     PKVGVWPDAYYVTFNMFTNFFTGARVCAWDRAAMIAGTAATQQCFQLSTSYGSLLPAD
                     MDGPVLPPTGSPNYMLNFTTNALRMWKFSVNWTNPAASTLTGPTSIPVAAFTRGCDGG
                     ACVEQPGTNQKLDSLGDRLMYRLAYRNFGTHQNLVVNHSVRTGQNRKTYRTGIRWYEL
                     RNLSAATPTVHQQGTYAPDTTNYRWMGSAAMDKVGNLAVGYSVSSSSLRPSIRFAARA
                     SSDPLGTLSTESNVLTGTGSQTQGLARWGDYATISIDPVDDCTMWFTTEYIATNGTWN
                     WHTRINSFKLQGCQ"
     gene            complement(4008..>4564)
                     /locus_tag="VF240_03390"
     CDS             complement(4008..>4564)
                     /locus_tag="VF240_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7174310.1"
                     /translation="STSDTATNTIGGTSMATPHVAGVAALYLQNNPSASPATVSGQII
                     STATTGVLTGTGTGSPNRLLYSLLTTGGGGGGGAPAACAGGTSATGTLSFTGDYDYHP
                     GGSYYYSAVSGYHKGCLVGPTSGADFDLYLQRWNGSAWIIVASSESATSSETISYFGS
                     AAYYRWQVYSYSGTGNYTLWTLRP"
BASE COUNT          711 a         1358 c         1687 g          808 t
ORIGIN      
        1 gtcgccgtga cgacgaggcc ggacggcgcg gccgggcctg ccggtggcgc gctcaggtag
       61 tcgagcgcgg ccttcgcctg cacgatgccg tagccgaagg tggtgtcgcg acccgccgtg
      121 cccttgtcgc gcgccgtcgc cgtcagggcg ttgcgaatct gcgcgttagt ccaggagggg
      181 ttgtagctcc agacgagggc cgccaccgcg gagacgtgcg gcgtcgccat cgaggtgccg
      241 ttgaagtagt cgtagacgtt attgaacgtc tgatcctcgt agctggagac cgtgcccacg
      301 gagccggcct gcgcgagcgc cgcttgcccg tccgcctgac tgaggccgat ggccgggatc
      361 gtgctcgaat tgccttcgcc gagcgtggcg aagagaggct ccgcgagatt gttgtagatg
      421 acggccgccg cgccgccgcc cagttggacg ttgtgtacct tgtcgtagaa gctgatcgtg
      481 ccgcgctcgc agaggacgac tttgcccgtc cacgcgccgc cgcccgtcgt gtcgcacagg
      541 ccgccgccga cgagcgtggc ggtcacgccc gagggggtgc tgagcgccgc gtactcgacg
      601 tggtggccgg tgtaggtcgt gccgttcacg cccagcgttt gcgtgtcctt gtaggcgatg
      661 gacgagagcg tggcgacgcc gggcgcggcg atctccacgt cggcgttgac gttcgagaag
      721 gtggcgtgcg cctcggcgtt gtcaacggcc gcgaccgaga cgaccgacga gtagccggcc
      781 gggtagttgg tcgccgtgtt gccgtcgttg ccggcggcgg caatcgagag gacacctgcg
      841 ttgtaggcgt tctggaaggc gttctgctcc gtgacgctgc cgcctaaacc gccgaggctc
      901 atgctgacga cgttcgagcc ggcgtcgcgg cactgctgga cggccgccgc gaggtcggaa
      961 gagtaggccc acgcgccgtt gtcgccgaag actttgacga tgtggaggtt gacggtgccg
     1021 ctcggcagga cgccgacgac gcccgtgccg ttacgcaggc cggcgatagt gccggcgacg
     1081 tgcgtgccgt ggctgtcgta gtcgcggtag gggtcgcccg tgcccgcgtt ggtcgaggcc
     1141 gtgacgtggc cgctcgtgcg atccatcaaa tcctcgtgcg cgtccttata gccggagtcg
     1201 atgatgcaga ctttgcggtt ggcggcgagc gagtcgctga cctgatcggc ctgcaccatc
     1261 tggatgccgt agggcgtggt ctcgttcgtg cccgcgatgg gcagatcgct ccacggcgcg
     1321 ccggtctgca tcgggtagcg gcgcgggtca acctcgatga actcgacgtt ggggttgttc
     1381 tggagggcga cgcgcgcgtg ctcgggcagg cgcgcggcga ccgacgcgag ttcgggaagc
     1441 tcgcgaacta cctggccgcc cctggcgcgc acgtcgtccg aggcgaactt gccgaactgt
     1501 cgaaacttga tgatgaaacg ctcgtcagca gaattattgt tggcggcggc cgcgtcggcg
     1561 gcccggtgtg aggggttgag gccggagcgt tgggcgctgg cgaggccgaa agggagcgcc
     1621 agggcacacg tcaccgccag cgcgaggaag gcaaaaaact ttttcattaa tcctccgaag
     1681 tgtaagggtg aatggtggtg aggaattccg cccgcttgtg atggaaaccc cttgtcctat
     1741 ccgcaaagat agcgcccgac tatgtatgaa aaaaccctgc ttggaagatg aacacgcatt
     1801 cgatgcgacg gggcggagtt tgattgcacg cgggcgcggc aattctttgc gaagcgactt
     1861 ccgaatcgtg tgtacgggac agtaccactc gcggtaactc gcgcgagcag cgttcgcgga
     1921 ctcaaccctt gactcgcttt gctccagcga tggcggattg gtccgcggaa aaacgaaggg
     1981 cgccgccgct ttgattgcgg cagcgcccaa cgtgtgggtc aaatgaggcg gacgttttac
     2041 tggcagccct gaagcttgaa gctgttgatg cgggtgtgcc agttccaggt gccgttggtg
     2101 gcgatgtact cggtcgtaaa ccacatcgtg cagtcgtcca ccgggtcgat gctgatggtg
     2161 gcgtagtcgc cccagcgcgc gaggccctgg gtctgcgagc cggtgccggt caggacgttc
     2221 gactccgtgc tgagcgtgcc gagcgggtcg gaggaggcgc gcgcggcgaa gcggatcgac
     2281 ggcctgagcg agctggagga gacgctgtag ccgacggcga ggttgccgac cttgtccatc
     2341 gcggccgaac ccatccagcg gtagttcgtc gtgtcgggcg cgtaggtgcc ctgctggtgg
     2401 acggtgggag tcgcggcgct caggttgcgc agctcatacc agcggatgcc ggtgcgatag
     2461 gtcttgcggt tctggcccgt ccgcaccgag tggttgacga cgaggttctg gtgcgtgccg
     2521 aagttgcggt aggcgaggcg atacatgagg cggtcgccga gcgagtcgag cttctggttg
     2581 gtgccgggct gctcgacgca ggcgccgccg tcgcagccgc gcgtgaaggc ggccacgggg
     2641 atggaggtcg gccccgtcag cgtggaggcg gcggggttcg tccagttgac gctgaacttc
     2701 cacatccgca gcgcgttggt cgtgaagttg agcatgtagt tgggcgagcc ggtggggggc
     2761 agcacggggc cgtccatgtc ggcgggcagg aggctgccgt aggaggtgct gagctggaag
     2821 cactgctgcg tggccgccgt gccggcgatc atcgcggcgc gatcccaggc gcagacgcgc
     2881 gcgcccgtga agaagttggt gaacatgttg aaggtgacgt aataggcgtc gggccagacg
     2941 cccaccttcg ggtagtcggg gaactggtcg tactggaagg cgtagcggtt gtaggagccg
     3001 gtggcgtccg aggtggtcga gacggcgacg cactgccagt agccggccgc accgcccggc
     3061 acggcgaact gcgtcatcac ccagcggttg gcgatctggt cgtactgcac gatggggtcg
     3121 ccgtcgtttc gggtctggca cgcgccgccg aagccggccc agagcgtgtt gcccgcggcc
     3181 gggccgtaag tcatcgcgcc cgagaccttg tcgaagacgg cgaagtggct gttgacccac
     3241 tggacgtact gcgtggcgcc gacatcaccc gtcgtgtcag gcggggcgac gttggcgttg
     3301 tagccgggca gcccctcgcc gacgccctcg aagttgagcc ccgcggtgat ggcgacgagc
     3361 gggacggccg atgactggac gacggggtca ttgaactcac cgcctggctc ggccgggcgc
     3421 ttcggctcga agtccgtgcg gctggctccg cccttgaccg gcgtgatctc gcgcagcgga
     3481 cgtgaggtgt cgtgcttgac ggcctttgcg gccgggagct tgtcgatcct gccctggccg
     3541 aatactagcg tcggtagtaa gagaaccgcg cacagagtga acagtgaagt caaaactttc
     3601 ttcattttac ctccggtaaa ggttgacaga gttgagggtg ggcgaggaac gctgtcaggc
     3661 gaccccggtt gctcgttgat taaagccgtc ggcggaaaag taagtccgac gttcactaac
     3721 accccgaaat gaactgtcac accacggccg ccggggccgc cccttgatgg attaagacaa
     3781 gatgcgcttt tcgtcctgac tttgaataca gtcggagacg cgtgagagcg ggaagtatag
     3841 gcccgccgcc ccgctgctgc aagcggccgg ggccgccgcc gcactcaaag tttcgacctg
     3901 aaccggcgcg cgcgtcgtcg tggcggccga cgaaattttt gcccgcgccc gccgccgcgg
     3961 gacggaagcc tgcggcggcg ggcggcgggg tttcggtcgc gtcggcccta cggccgcagc
     4021 gtccagaggg tgtagttgcc cgtgccgctg taggagtaga cctgccagcg gtagtaggcc
     4081 gcggagccga agtaggagat ggtctcgctg gaggtggcgc tctccgagct ggcgacgatg
     4141 atccacgccg agccgttcca cctctgtaaa tagaggtcga agtccgcgcc cgaagtcggc
     4201 ccgaccagac agcctttgtg atagccggag accgcgctgt agtagtagct gccgccgggg
     4261 tgatagtcgt agtcgcccgt gaaactgagc gtgcccgtgg cggacgtgcc gccggcgcag
     4321 gccgccgggg cgccgcctcc tcctccgccg cctgtggtga gcagcgagta gagcagacgg
     4381 ttgggcgacc ccgtccccgt gccggtgagc acgccggtcg tcgccgtcga gatgatctgg
     4441 ccggagacgg tcgcgggcga ggccgacggg ttgttttgca gatagagcgc cgcgacaccc
     4501 gcgacgtgcg gcgtggccat cgacgtgccg ccgatagtgt tcgtcgccgt gtcggacgta
     4561 gacc
//
LOCUS       DASVES010000094        10748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_297513, whole genome shotgun sequence.
ACCESSION   DASVES010000094 DASVES010000000
VERSION     DASVES010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297513"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VF240_03395"
     CDS             <1..1124
                     /locus_tag="VF240_03395"
                     /inference="COORDINATES: protein motif:HMM:NF045283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX7174311.1"
                     /translation="VRSLRLLSGSTSPLESLLQEVSRQTNLSGADGDGVWAWLKGFFT
                     SKTGIGVTPVDKEFRPLVQFVSGKADASPMAEHRAQLKKVADALNANNKPLSELSKAM
                     QAGDDTIGLRPARQAVADSLEAKGFSSSPASGDAARVLRLPLDTLNTLLVGTDFEQIE
                     KAWAALFAKAQGFEAGFPFADGGSDTSLAALAQFLNPQDGELTRFFQERLKPYFEDDW
                     TVKKEAADKFAPAFVSYLASARRLREALFPDNGRQPKAEYQLTLLPARDALVRVEVDG
                     QLLQTPDKTTANMSWPGDKSGVRITVTPTNGQDAVKAFAGEWGLLRMFRESGGGAGSG
                     ATFTLQPAAGVTLSLQPKSGNPFKRELFSALKAPKGVRQ"
     gene            1268..2104
                     /locus_tag="VF240_03400"
     CDS             1268..2104
                     /locus_tag="VF240_03400"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEX7174312.1"
                     /translation="MAGQLAATQAKALYDSGDLSAAIDEITREVKANPTDTARRSFLF
                     ELLCFAGEWERAERQLGALGREGVGAEVGAQVFRNCLRAELDRQRLVRDGLAPHFLAD
                     PPDYIDLHLDAFNRLRAGDAAGARASLDRAEEQRPALSGTLDDKLFTDFRDYDDFFAP
                     VLELIVHDKYTWVPYEQIRRVEIEPPVQLRDLVWATVEVETADKEMKAMTPTRYVDSG
                     AHQNDLVRLGRMTDWRELGPELYAGAGLRLFAVDGEERPALSVRSLVFDAPGDGEETP
                     AS"
     gene            2156..3325
                     /gene="tssA"
                     /locus_tag="VF240_03405"
     CDS             2156..3325
                     /gene="tssA"
                     /locus_tag="VF240_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEX7174313.1"
                     /translation="MSSAMETNTAPAATNSMPPVVDVEALLAPIPGDNPAGESLAYSG
                     LYDEIKEARRADDTLEQGDWVRDTKSADWVRAATLATDALTSQTKDLQICAWMTEALT
                     KLYGFAGLRDALKVVHGLHVGFWDTLFPEEDDGDLEGRANAISWMEREAAVALREVPI
                     TRGAGGQSYGFNEFEQSNAFNVGPEVSPEVAEERRRRAGEEGKITSEDWLKAKQSTPR
                     AFYEAAHATLDEAWEYFQALDRLLDERYKNQAPAMTELKKALDSVRAAVEKIVKEKRL
                     LEPDPISVGEAAGGEGEAGANGGAPGGGATAGGVFISGPIRTRAEAVSRLTEVAAFFR
                     QTEPHSPVSYLVERSVKWSQMPLEQWLASVIKDDTVLGALRETLGVDLDNSQAEE"
     gene            3469..4005
                     /gene="tssB"
                     /locus_tag="VF240_03410"
     CDS             3469..4005
                     /gene="tssB"
                     /locus_tag="VF240_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEX7174314.1"
                     /translation="MPKTESTQHKLSRVRAPRVHLTYDVEVGNAIEAKELPFVVGVLG
                     DFSGKPDEPLPRVKDRKFIEIDRDNFDKVLSGMKPRLSYSVENKLTGDGGKLGVDLRF
                     NSLEDFEPDRVVQQVEPLRKLVEARRRLSDLVSKMDGNDKLEGLLSDIISNTGAQKQL
                     GKELGLGEDGQKKEEPNE"
     gene            3998..5482
                     /gene="tssC"
                     /locus_tag="VF240_03415"
     CDS             3998..5482
                     /gene="tssC"
                     /locus_tag="VF240_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX7174315.1"
                     /translation="MSSKKDAAQAETVTQEAQGDLLSRILSEGRLARRDDEQAHARDM
                     VAEFVSQVMQGQLTMSRDMEATINARIAAIDKLLSAQLNEVMHHEDFQKLEGSWRGLN
                     HLVMNSETGTMLKIRVFNVSKKELLKDLEKAAEFDQSALFKKVYEEEFGTFGGAPFGA
                     LIGDYEFSSHPQDLSLLEKISNVAAAANAPFTAAASAQMFGWDSFTDLGEVRDLAKIF
                     DRTEFAKWRSFRESEDSRYVGLTLPHTLMRLPYGQDTTPTETFRFEEDVDGRDHKKYL
                     WGNAAYSFGTRLTASFAKYQWCATIRGVEGGGLVQGLPTHTFKTDDGDVALKCPTEIA
                     ITDRREKEFSDLGFIPLVHCKGTDYAAFFAAQSAQKAKKYDSDAANANARLSTQLPYI
                     FAVSRFAHYLKAMMRDKIGSFMERSECEHFLNTWISQYTVSNDTAGPEIKAQKPLREA
                     RIEVKEVPGKPGAYRAVAFLRPHFQLDELTVSLRLVAELPQSAR"
     gene            5608..6090
                     /locus_tag="VF240_03420"
     CDS             5608..6090
                     /locus_tag="VF240_03420"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX7174316.1"
                     /translation="MAVDMFIKIGALEGESSDKAHGKEIDVLAWSWGMSNSGSAHHGG
                     GQGAGKVNVQDISLTKYIDKSSPDLMLACCNGKHFPEAKLVVRKAGEKPLEYLVVTMT
                     DLIITSVSTGGSGGEDRLTENVTLNFAKVKVDYKEQTKTGGVGATPTMGWDIAANTKM
                     "
     gene            6193..6678
                     /gene="tssE"
                     /locus_tag="VF240_03425"
     CDS             6193..6678
                     /gene="tssE"
                     /locus_tag="VF240_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEX7174317.1"
                     /translation="MARIDNEVRITLSVLDRLLDYEPEVSYEPPASRAKSMRLFRQAV
                     RRDLEWLLNTRRVATPLPEGLKEVNNSLAAYGLPDFTAANTGDTDDQAQVRAAIEETI
                     RTFEPRLQDVVVTMEPRHETERSLRFRIDAVMMVDPAPEPVTFDTTLQLVSGEYKVQE
                     K"
     gene            6678..8585
                     /gene="tssF"
                     /locus_tag="VF240_03430"
     CDS             6678..8585
                     /gene="tssF"
                     /locus_tag="VF240_03430"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX7174318.1"
                     /translation="MRDDLLTYYERELAFVRQMGAEFAQKYPKIASRLLLEPDKCEDP
                     HVERMIEAFAFLAARVHLKIDDEFPEITESLLNVLYPHYLAPIPSLSIVQFALDPEQG
                     KLTTGYHIPRDTALYSRPIQGTPCRFRACYPVTLWPVEVAAAALESLDPVDTRGRWQE
                     AVLRIKLRCVNNTKLSELKTGEGDSSGSGDNAPRPIERLRFYLSGEPQLTYALYEMIF
                     NNATRVELRSEGKRAPGRAAPAVITLPPSSLKPVGFAPDEGLLPYTRRSFAGYRLLTE
                     YFTFPEKFLFFDLTGLDEAVRRGGFGEEFSVSIFVRDVQPPRALVSAATFQLGCAPVV
                     NLFRKVAEPIQLTQLQHEYRVVPDVHRQMATEVYSVDSVSSTDPYLKQSRQYEPFYSI
                     RHAESAEREQTFWYASRRLSPRKDDAGTEIYLSLVDLGFNPRVPAVETVTATVTASNR
                     DLPAKLPFGGRDGDFEVEGTGPLARARCLRKPTPTVRPPLRRGAQWRLISHLSVNHLS
                     IMGDADGGGADGAAADALREILSLYDFTDSPATRRQIRGITGVAGRRVVRQTGSRVGA
                     GFVRGLETTVEFDEEMFVGSGVYLFSSVLERFLALYATANSFNQLVAKTAQREEVLKR
                     WPPRAGHQAIL"
     gene            8549..9616
                     /gene="tssG"
                     /locus_tag="VF240_03435"
     CDS             8549..9616
                     /gene="tssG"
                     /locus_tag="VF240_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX7174319.1"
                     /translation="MAAEGRAPGDTLEQTLDTLEQTLLDEPYRFRFFQAVRVVERLHP
                     ERASVGRQEAAPGREAVRFRTHPSLQFPPSEIHEFSRDGDNDGALETDAPPPEMTVAF
                     MGLTGPQGVLPNHTTELVAERARYKDTALWEFLDLFSHRMISLFYRAWERYRFPVAFE
                     RGTHDEFTRHLFALVGMGTEGLRGRLGLPDEGLLLYAGLISQRPHSGVAVESALGDYF
                     RVPARLVPFTGQWLALDDESLCRLGRANSALGVSTIAGARVWDTQSKFRMRFGPLTFS
                     EFTAFLPSGRNYGAAVRLTRFLAGAELDFDAQLVLKAEEVPACVLSAAGPNRPALGWT
                     TWLKTQPTDRDDSQVVLAARN"
     gene            9699..>10748
                     /locus_tag="VF240_03440"
     CDS             9699..>10748
                     /locus_tag="VF240_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7174320.1"
                     /translation="MEVNFLRSLIGRLDETCTNALQAAAGLCLARTNYDVDVEHLLLK
                     LLETSGTDIERILRHYEINQGRVVNDVTRALDKLKTGNTRTPDFSPRLPQLFQEAWLH
                     ASIEYGAARVRSGHVLLALLTSENLSRLAREISKEFAAISVESLKKNLPDIIAGSTED
                     REAGALGSAQGTSAAGAGGGDAAGATPSAVGGKQKALDQFTVDLTGKAREGKIDPVLG
                     RDFEIRQVVDILTRRRQNNPILTGEAGVGKTAVVEGFAQRVADGDVPEPLRNVAVRTL
                     DLALLQAGAGVKGEFENRLKGVIEEVKSSPQPIILFIDEAHTMIGAGGQAGQNDAANL
                     LKPALARGELRTIAAT"
BASE COUNT         1942 a         3682 c         3502 g         1622 t
ORIGIN      
        1 ccgtccgaag cttgcgcctg ctctcgggga gcacgtcgcc gctggagagt ctgctgcaag
       61 aggtctcgcg gcagacgaac ctctcgggcg cggacggcga cggcgtctgg gcctggctca
      121 agggcttctt cacgtcgaag accggcatcg gcgtcacccc cgtcgataag gaatttcgcc
      181 cgctcgtgca attcgtctcg ggcaaggcgg acgcttcgcc gatggccgaa caccgcgcgc
      241 aactcaagaa ggtggccgac gccttaaacg ccaacaacaa gccgctctcg gaactctcca
      301 aggcgatgca ggccggcgac gataccatcg gcctgcggcc cgcgcgtcag gcggtcgccg
      361 actcgctcga agccaaaggc ttcagctcct cgccggcgag cggcgacgcc gcgcgcgtcc
      421 tgcgtctgcc gctcgacact ctgaacacgc tgctcgtcgg caccgacttc gagcagatcg
      481 agaaggcgtg ggcggcgctc tttgcgaagg cgcaaggctt cgaggccggc ttcccgttcg
      541 ccgacggcgg ctcggacacc tcactcgccg cgctcgcgca gttcctcaac ccgcaggacg
      601 gcgagctgac gcgcttcttc caggagcgcc tgaagcctta cttcgaggac gactggacag
      661 tgaagaagga ggccgcggac aagttcgcgc ccgccttcgt ctcctatctc gccagcgcgc
      721 ggcgtctgcg cgaggcgctc ttccccgaca acggccgaca acctaaggcc gagtaccagt
      781 tgacgctcct gccggcgcgc gacgcgctcg tccgcgtcga ggtggacggc caactgctcc
      841 agacgccgga caagacgacg gcgaacatgt cttggccggg cgacaagtcg ggcgtgcgaa
      901 ttacggtgac gccgacgaac gggcaggacg ccgtcaaggc tttcgccggc gagtggggcc
      961 tgctgcggat gttccgcgag agcggcggcg gcgcggggag cggggcgacg ttcacgctcc
     1021 agccggccgc cggcgtcacc ctctcgctcc agcccaagag cggcaacccg ttcaagcgcg
     1081 aactcttcag cgcgctcaaa gcgccgaagg gcgtgcggca gtagtagcaa ggagctgtta
     1141 gctgttagct gttagctttc agccagaagt gagtggacga atgagtttga agtatcaggc
     1201 cggcggccgg cgactggcta acagctaatc gctaacagct aacagctaca agaaaggcgg
     1261 cgtaaggatg gcgggacagt tggcggcgac acaggccaaa gcgctctacg actcgggcga
     1321 cctctcggcg gccatcgacg agatcacgcg cgaggtgaag gccaacccga ccgacacggc
     1381 gcggcgctcc ttcctcttcg agctgctctg cttcgccggc gagtgggagc gcgccgagcg
     1441 ccaactcggc gcgcttggcc gcgagggcgt cggcgcagag gtcggcgcgc aggtcttccg
     1501 caactgcctg cgcgcggaac tcgaccgcca gcgcctcgtg cgcgacggcc tcgccccgca
     1561 cttcctcgcc gacccgcccg actacatcga cctccacctc gacgccttca accgcctgcg
     1621 cgccggcgac gcggccggag cacgcgcctc gctcgaccgg gccgaggagc agcgcccggc
     1681 gctctcagga acgctcgacg ataagctctt caccgacttc cgcgactacg acgacttctt
     1741 cgcccccgtg ctcgaactaa tcgttcacga caagtacacg tgggtgccct acgaacagat
     1801 caggcgcgtg gaaatcgagc cgcccgtcca actgcgcgac ctcgtgtggg cgaccgtgga
     1861 ggtggagacg gccgacaagg agatgaaggc gatgacgccg acgcgctacg tcgattcggg
     1921 cgctcaccaa aacgacctcg tccggctcgg gcgcatgacc gactggaggg aactagggcc
     1981 ggagctttac gcgggcgcgg gcctccgcct cttcgccgtt gacggcgagg agcgccccgc
     2041 gctcagtgtc cgcagcctcg tcttcgacgc ccccggcgac ggcgaagaga cgccggcctc
     2101 gtaaggtaga ggccacacgc acgaagtcaa ccgtcccggt cagaggtatt acgcgatgtc
     2161 ttccgcgatg gaaaccaaca cggccccggc cgccaccaac tcgatgcccc cggtcgtcga
     2221 tgtcgaggcg ctgctcgcgc ccatccccgg cgacaacccg gcgggcgaaa gcctcgccta
     2281 ctccggcctc tacgacgaga taaaagaggc gcgccgcgcc gacgacacac ttgaacaggg
     2341 cgattgggtg cgcgacacga agtcggccga ctgggtgcgg gcggcgacgc ttgcgaccga
     2401 cgcgctcacg tcgcagacga aggatttgca aatctgcgcg tggatgaccg aggcgctcac
     2461 gaagctctac ggcttcgcgg gtctgcgcga cgcgctcaag gtcgtccacg gcctgcacgt
     2521 cggtttctgg gacacgctct tccccgagga ggacgacggc gacctcgaag ggcgcgccaa
     2581 cgccatctcg tggatggagc gcgaagcggc cgtcgccctg cgcgaagttc cgatcacgcg
     2641 cggcgcgggc ggtcagagct acggcttcaa cgagttcgag cagtcgaacg ccttcaacgt
     2701 cggccctgag gtgagtcccg aggtggccga ggagcgacgc cgtcgcgccg gcgaggaggg
     2761 caagatcacc agcgaggact ggctcaaggc caagcagtcc acgccgcgcg ccttctacga
     2821 ggcggcccac gccacgctcg acgaggcgtg ggagtatttt caggcgctcg accgcctgct
     2881 cgacgagcgc tacaagaatc aggcgccggc gatgaccgag ctgaagaagg cgctcgactc
     2941 ggtgcgcgcc gccgtcgaga agatcgtcaa ggagaagcgc ctgctggagc ctgacccgat
     3001 aagcgtgggc gaggccgcgg gcggcgaagg cgaggccggc gcgaacggcg gagcgccggg
     3061 cggcggggcg acggcgggcg gcgtctttat ctcggggccg atcaggacgc gggccgaggc
     3121 cgtcagtcgt ctcaccgagg tggccgcctt cttccgccag accgagccgc acagccccgt
     3181 ctcctacctc gtcgagcgct ccgtcaagtg gagccagatg ccgcttgagc agtggctcgc
     3241 cagcgtgatt aaggacgaca cggtgctggg cgcgctgcgc gagacgctcg gcgtcgatct
     3301 cgacaacagt caggccgagg agtgagagcg ccggccgtgg cggcggcggt gatgatgacg
     3361 atgtcggtgg tgtcggtgtt gtcggccgcc agttaagttt cccccggccg cggccgggcc
     3421 gccgggccac aaaccggaaa gttaaaatgt gagaaaggaa aaagcagtat gccaaagact
     3481 gaaagcacac aacacaagct gagtcgcgtc cgcgccccgc gcgtgcatct cacctacgac
     3541 gtggaggtgg gcaacgcgat tgaggcgaag gaactgccgt tcgtggtcgg cgtgctcggc
     3601 gacttctcgg gcaagcccga cgagcctttg cccagggtca aggatcgcaa gttcatcgaa
     3661 atcgaccggg acaacttcga caaggtgctc tcggggatga agccgcgcct cagttacagc
     3721 gtcgagaaca agctgacggg cgacggcggc aagctcggcg tcgatctgcg cttcaacagt
     3781 ctcgaagact tcgagcctga ccgcgtcgtg cagcaggtcg agccgctccg caaactggtc
     3841 gaagcgcgcc gccgcctctc cgacctcgtc tcgaagatgg acggcaacga caagctcgaa
     3901 gggctgctct ccgacatcat ctcgaacacg ggcgcgcaga agcagctcgg caaggagctg
     3961 gggcttggcg aagacggcca gaagaaggag gagccaaatg agtagtaaga aagacgcggc
     4021 gcaggccgaa accgtcacgc aggaggcgca gggcgacctg ctctcgcgca tcctctccga
     4081 gggtcgtctc gcccggcgcg acgacgaaca ggcgcacgcc cgcgacatgg tcgccgagtt
     4141 cgtctcgcag gtgatgcagg ggcagttgac gatgtcgcgc gacatggagg cgaccatcaa
     4201 cgcccgcatc gcggccatcg ataagctgct ctcggcgcag ctcaacgagg tcatgcacca
     4261 cgaagacttc cagaagctcg aagggtcgtg gcgcggcctc aaccatctcg tgatgaactc
     4321 cgagacgggg acgatgctca agattcgcgt cttcaacgtc tcgaagaagg agctgctcaa
     4381 agatttggag aaggccgcgg agttcgacca gtcggccctc ttcaagaagg tctacgaaga
     4441 agagttcggc accttcggcg gcgcgccctt cggggcgctc atcggcgact acgagttctc
     4501 cagccacccg caggacctct cgctgctgga gaaaatctcg aacgtcgccg cggccgccaa
     4561 cgcgccgttc acggccgccg cctcggccca gatgttcggc tgggacagct tcaccgatct
     4621 gggcgaagtg cgcgatctgg cgaagatttt cgaccgcacc gagttcgcca agtggcgctc
     4681 cttccgcgag tcggaagatt cgcgctacgt cggactcacc ttgccgcaca cgctgatgcg
     4741 tctgccctac ggtcaggaca ctacgccgac cgagaccttc cgcttcgagg aagacgttga
     4801 cggccgcgac cacaagaagt acctgtgggg caacgccgcc tattccttcg gcacgcgcct
     4861 gaccgcgtcg ttcgccaagt atcagtggtg cgcgaccatc cgcggagtgg agggcggcgg
     4921 actcgtacaa ggtttgccca cccacacctt caagaccgac gacggcgacg tggccctgaa
     4981 gtgtccgacc gaaatcgcca tcaccgaccg ccgggagaag gagttctccg acctcggctt
     5041 catcccgctc gtccactgta aggggacgga ctacgccgcc ttcttcgccg cgcagtcggc
     5101 gcagaaggcc aagaagtacg actccgacgc ggcgaacgcc aacgcccggc tctccacgca
     5161 gttgccatac atcttcgccg tctcgcgctt cgcccactac ctgaaggcga tgatgcgcga
     5221 caagatcggc agcttcatgg agcgcagcga gtgcgagcac ttcctcaaca catggatttc
     5281 gcagtacacc gtctcgaacg acacggccgg cccggaaatc aaggcgcaga agccgctgcg
     5341 ggaggcgcgc atcgaggtca aggaggttcc gggcaagccc ggcgcctaca gggcggtggc
     5401 gttcctcagg ccgcacttcc agttggacga gttgacggtc tcgctgcgcc tggtggccga
     5461 gctgccgcag tcggcgcggt gatggtcgcc gactccgacc gaagtaaacc agacgcgtct
     5521 tgcgccgggg gcgcgcaggc agcaaccccc gcctcgccca cggcaggccg ctttctcgag
     5581 agataaaaat aggaggacta aaagctcatg gcagtagata tgttcatcaa gatcggcgcg
     5641 ctcgaaggcg agtcgtcgga caaggcgcac gggaaggaga tcgacgtgct ggcctggagt
     5701 tgggggatgt cgaactcggg cagcgcccac cacggcggcg gtcagggcgc cggcaaggtc
     5761 aacgtgcagg acatcagcct gacgaagtac atcgacaagt cgagccccga cctgatgctc
     5821 gcctgctgta acggcaagca cttccccgag gccaagctcg tcgtccgcaa ggccggcgag
     5881 aagccgctcg aatacctcgt cgtgacgatg accgacctga tcatcaccag cgtctcgacg
     5941 ggcggctcgg gcggcgaaga ccggctcacc gagaacgtca cgctcaactt cgccaaggtc
     6001 aaggtcgatt acaaggagca gaccaagacg ggcggcgtcg gcgccacccc cacgatgggg
     6061 tgggacatcg ccgcgaacac caagatgtaa cgtcgcgcgc cggggggtta actcgccgga
     6121 gtagacggaa gtggtgtcac cgggcgtgtc ggcacgaggc aagcgccgac acgcccggcc
     6181 cctctcacgc tcatggccag aatcgacaac gaagtccgca tcacgctctc ggtcttagac
     6241 cgcctgctcg actacgagcc ggaggtctct tacgagccgc cggcgtcgcg cgccaaaagc
     6301 atgcgcctct ttaggcaggc cgtccggcgc gacctcgaat ggctgctcaa cacgcggcgg
     6361 gtggcgacgc cgctgcccga gggtctgaaa gaagtgaaca actcgctcgc cgcctacggc
     6421 ctgcccgact tcaccgccgc taacacggga gacaccgacg atcaggcgca ggtgcgcgcg
     6481 gccatcgagg aaaccatcag gactttcgag ccgcgccttc aggacgtggt ggtgacgatg
     6541 gagccgcgcc acgagaccga gcgtagctta cgcttccgca tcgacgccgt gatgatggtc
     6601 gatccggcgc ccgagccggt gactttcgac accaccctcc aactcgtcag cggcgagtac
     6661 aaggttcaag agaagtaatg cgcgacgacc tgctcacata ctacgagcgc gagctggcct
     6721 tcgtgcgcca gatgggggcc gagttcgcgc agaagtaccc gaagatcgct tcgcgtctgc
     6781 tcctcgaacc ggacaagtgc gaagacccgc acgtcgagcg catgatcgag gccttcgcct
     6841 tcctcgccgc gcgcgttcac ctgaagatcg acgacgagtt ccccgagatt accgagtcgc
     6901 tcttaaacgt cctctatcca cactacctcg cgcccatccc gtcgctttcg atagtgcagt
     6961 tcgcgctcga ccccgagcag ggcaagctca ccaccggcta ccacatcccg cgcgacacgg
     7021 cgctctactc gcgccccatt cagggcacgc cctgccgctt ccgcgcctgc taccccgtca
     7081 cgctctggcc cgtcgaagtg gccgcggccg cgctcgaatc gctcgacccc gtggacaccc
     7141 gcggccgctg gcaggaggcg gtgctgcgta tcaagctgcg ctgcgtcaac aacaccaagc
     7201 tctcggaact caagacgggc gagggcgaca gcagcggcag cggcgacaac gcgccgcgtc
     7261 cgattgaacg cttgcgcttc tacctgagcg gcgagccgca gctcacctac gcgctctacg
     7321 agatgatctt caacaacgcg acgcgcgtcg agctgcgctc ggagggcaag cgcgctccgg
     7381 ggcgcgccgc gccggccgtc atcacgctgc cgccttcgtc gctgaagccc gtcggcttcg
     7441 cgcccgacga gggactgctg ccctacacgc ggcgctcgtt cgccggctac cggctgctca
     7501 ccgagtactt caccttcccc gagaagtttc tcttcttcga cctcacgggt ctcgacgagg
     7561 ccgtccgccg cggcggcttc ggtgaagagt tcagcgtctc gattttcgtc cgcgacgtgc
     7621 agccgccgcg cgcgctggtc tcggccgcca cgttccaact gggctgcgcg cccgtcgtca
     7681 acctcttccg caaggtggcc gagccgattc agttgacgca gttgcagcac gagtaccgcg
     7741 tcgtccccga tgtgcaccgg cagatggcga ccgaggtcta ctcggtcgat tccgtgtcct
     7801 cgaccgaccc ctacctcaag cagtcgcgac agtacgagcc gttctattcg attcgccacg
     7861 ccgagtcggc cgagcgcgag cagaccttct ggtacgcctc ccgacgcctc tcgccgcgca
     7921 aggacgacgc gggcaccgaa atttatctct cgctcgtcga tctgggtttc aacccgcgcg
     7981 tgccggccgt cgagaccgtc acggcgaccg tcacggcgag caatcgcgac ctgccggcca
     8041 agcttccctt cggcgggcgc gacggcgact tcgaggtcga ggggacgggg ccgctcgcgc
     8101 gggcgcggtg cttgcgtaag cccaccccca cggtgcgccc gccgctgcgg cgcggcgcgc
     8161 agtggcggct catctcgcac ctctcggtaa accacctctc gattatgggc gacgccgacg
     8221 gcggcggcgc cgacggggcg gcggccgacg cgctgcgcga gattctctcg ctctacgact
     8281 tcacggactc gccggccacg cgccggcaga ttcgcggcat cacgggggtg gcgggccgac
     8341 gggtcgtgcg gcagacgggg tcgcgcgtcg gggcggggtt cgtgcggggc ttagagacga
     8401 ccgtcgagtt cgacgaggag atgttcgtcg gcagcggcgt ctatctcttc tcctccgtgc
     8461 tcgaacgctt cctcgccctc tacgccaccg ccaactcgtt caatcagctc gtcgcgaaga
     8521 cggcgcagcg tgaggaggtc ttgaagcgat ggccgccgag ggccgggcac caggcgatac
     8581 tttagagcag acgctcgata ctttagagca gacgctctta gacgagccgt accgcttccg
     8641 cttctttcag gcggtgcgcg tcgtcgagcg cctgcacccc gagcgcgcgt cagtcggccg
     8701 gcaggaggcc gcgccggggc gcgaggccgt gcgcttccgc acgcatcctt cgcttcagtt
     8761 tccgcccagc gagatacacg agttcagccg cgacggcgac aacgacggcg ctttggagac
     8821 ggacgcgccg ccgcccgaga tgaccgtcgc cttcatgggc ctgacggggc cgcagggcgt
     8881 gctcccgaat cacacgacgg aactggtggc cgagcgcgcc cgctacaagg acacggcgct
     8941 ctgggaattc ctcgacctct tcagccaccg catgatctcg ctcttctacc gcgcgtggga
     9001 gaggtacaga tttcccgtcg ccttcgagcg cggcacgcac gatgagttca cacgccatct
     9061 cttcgcgctc gtcggcatgg gcaccgaagg cttgcgcggg cggctcggtt tgccggacga
     9121 agggctgctc ctctacgccg gcctcatctc gcagcgcccg cactcgggcg tcgccgtcga
     9181 gtcggcgctc ggcgactact tccgcgtccc cgcccgtctc gtgccgttca cggggcagtg
     9241 gctcgcgctc gacgacgaaa gcctctgccg gctgggacga gccaacagcg cgctcggcgt
     9301 gtcaaccatc gccggcgcgc gcgtctggga cacgcagtcg aagttccgca tgcgcttcgg
     9361 gccgctcacg ttctccgagt tcacggcctt cctcccctcg gggcgaaact acggagcggc
     9421 cgtgcggctc acgcgctttc tcgccggggc ggaactcgac ttcgacgcgc agctcgtcct
     9481 caaggcggag gaagtcccgg cctgcgtcct gagcgccgcg gggccgaacc gccccgcgct
     9541 cggctggacg acctggctca agacgcagcc caccgaccgc gacgactcgc aggtcgtgct
     9601 cgcggcgaga aactgactac tgacaagtgc tcacggcttc aggcaatcgt agacagtcat
     9661 cagacccttt gatttaaacc cccgagagga gtaaggccat ggaagttaac tttctgagat
     9721 cgctcatcgg caggctcgac gagacgtgta cgaacgcgct gcaagcggcc gccgggctgt
     9781 gtctggcgcg caccaactac gacgttgatg tcgagcacct gctgctcaag ctgctggaaa
     9841 cctcgggcac cgacatcgag cgcatcctcc gccactacga gatcaatcag gggcgcgtgg
     9901 tcaacgacgt gacgcgcgcc ctcgacaagc tgaagaccgg caacactcgc acgcccgact
     9961 tctcgccgcg cctgccgcag ctctttcagg aagcatggct ccacgcctcc atcgagtacg
    10021 gcgcagcccg tgtgcgctcc ggccacgtcc tgctcgcgct cctgacgagc gaaaacctct
    10081 cgcgtctcgc gcgcgaaatc tcgaaggagt tcgcggccat ctccgtcgag tcgctgaaaa
    10141 agaatttgcc ggacatcatc gccggctcga ccgaagaccg tgaggccggc gcgctcggct
    10201 ccgcgcaggg gacatccgcg gccggcgcgg gcggcggcga cgccgcgggt gcgacgccca
    10261 gcgcggtcgg cggcaagcag aaggcgctcg accagttcac cgtcgatctc acggggaagg
    10321 cgcgcgaagg caagatcgac cccgtcctcg gccgcgactt cgagattcgt caggtcgtgg
    10381 acatcctcac gcgccgccgc cagaacaacc cgatcctgac gggcgaggcg ggcgtcggca
    10441 aaacggccgt cgtcgagggc ttcgcgcagc gcgtggccga cggcgacgtg cccgagccgc
    10501 tgaggaacgt cgccgtccgc acgctcgacc tcgcgctctt gcaggccggg gccggcgtca
    10561 agggggagtt cgagaatcgc ctgaagggcg tcatcgagga agtgaaatcg tcgccgcagc
    10621 ccatcatcct cttcatcgac gaggcgcaca cgatgatcgg ggcgggcggg caggcgggcc
    10681 agaacgacgc ggccaacctc ctcaagcccg cgctcgcgcg cggcgagctg cgcaccatcg
    10741 ccgccacg
//
LOCUS       DASVES010000095         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1402648, whole genome shotgun sequence.
ACCESSION   DASVES010000095 DASVES010000000
VERSION     DASVES010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402648"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VF240_03445"
     CDS             <1..949
                     /locus_tag="VF240_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174321.1"
                     /translation="ILLCGVAAASAHAQTDASLVGTWQAKGPDGPVVVKLNADGTGSL
                     DGTPFSYVVRGDKIDVTANGVTTPYDFKLTGGVLSVSGAGQSYIFERVGGGEGKASGV
                     ADEKSSARARNPLVGRWLSDEDTVDIREDGTLYLSGMKMTYKIEGDRITVSTGEGDMV
                     FPFRMKGDDKFSVTIEGREREYDREKEGPGGGGGLKVSEGAGANPRELAGKWCYMANV
                     NNYAGGRISNRCFTLREDGTYDFNAESSSSGPVASSASQESDTGTWTATATTITSRSS
                     KTGTNIYKLERRNHPKNGDPMLVLDGDAYVTATQRPSWP"
     gene            complement(1028..1492)
                     /locus_tag="VF240_03450"
     CDS             complement(1028..1492)
                     /locus_tag="VF240_03450"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7174322.1"
                     /translation="MTNEEIARRFERLAVLMSIRGDDQFRVRSYRNAAEIIETWPTPL
                     AKIAKEEGVKGLQTLPSVGKAISARVVELLERGTFEAWEKLTAETPETVLDLLEVEGV
                     GLKTAATLHQQFKISSLEDLRNFVEGGGLEMVDGVGEKSAERIARSVRGRQK"
     gene            complement(1584..>2390)
                     /locus_tag="VF240_03455"
     CDS             complement(1584..>2390)
                     /locus_tag="VF240_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX7174323.1"
                     /translation="YDREEGGGLRDFIDAAALSSDQDQYERASPVTLMTIHSAKGLEF
                     PVVFLVGMEDGLFPHSRSAADADELEEERRLAYVAITRAERFLYVSHAMKRRVYGEEM
                     ASEPSQFLNEMPFELMEDLSRGPSWLSFARSSSAAESRHVARALRGETGAPAAEKKYA
                     GKTYDSADSIAEFFRQRGEKFKGASSPPIVPEPKRRAASATGGGSSASSASTSGGGGF
                     VPGSHVRHPKYGRGLVLRREGVGDSTKLTVSFPGFGQKKLIEKYAGLEKA"
BASE COUNT          429 a          785 c          784 g          392 t
ORIGIN      
        1 catcctgctg tgcggcgtcg ccgccgccag cgcacacgcg cagactgacg cgagcctcgt
       61 cggcacgtgg caggcgaagg ggccggacgg ccccgtcgtc gtcaagctga acgccgacgg
      121 gacgggcagc ctcgacggca cgccgttcag ctatgtcgtg cgcggcgaca agatcgacgt
      181 cacggcaaac ggcgtaacca cgccttacga cttcaagctg acggggggcg tgctgagcgt
      241 cagtggcgcc ggccagagct acatcttcga gcgcgtcggc ggcggcgaag ggaaggcgag
      301 cggcgtcgct gacgaaaagt cttcggcgcg cgcgcgcaac ccgctcgtcg gccgctggtt
      361 gagcgacgaa gacacggttg acatccgcga ggacggaacg ctctacctga gcggcatgaa
      421 gatgacctac aagatcgagg gcgaccgcat cacggtctcc accggcgagg gcgacatggt
      481 cttccccttc cgcatgaagg gcgacgacaa gttcagcgtg acgattgagg gccgggagag
      541 agaatacgac cgggagaagg aagggccggg cggcggcggc ggcctcaagg ttagtgaagg
      601 cgcgggcgcg aacccgcggg agctggccgg caagtggtgc tacatggcga acgtgaacaa
      661 ctacgccggc gggcgcatct cgaaccgctg cttcacgctg cgcgaggacg gcacctacga
      721 cttcaacgcc gagtcgtctt cgagcggccc cgtcgcctcc tcggcctcgc aggagtccga
      781 cacgggcaca tggacggcga cggcgacgac catcacctcg cgctcaagca agaccggcac
      841 caacatctac aagctcgaac ggcgcaacca ccccaagaac ggcgacccga tgctcgtcct
      901 cgacggcgac gcctacgtca ccgcgaccca gcgcccctcg tggccgtgac tgaagagggc
      961 aaaagtaaaa aggcaaaagg caaaagtgga aaggcgaacc gccttcttac ttttgccttt
     1021 tgccttttta cttttgcctt cccctgacgc tccgcgcgat cctctccgcc gacttctcgc
     1081 ccacgccgtc aaccatctcc agcccgccgc cctcgacaaa atttcggagg tcttcgagcg
     1141 aagagatttt gaactgctgg tggagcgtcg ccgccgtctt cagcccgacg ccctcgactt
     1201 cgagcaggtc gagcaccgtc tccggcgtct cggccgtcaa cttctcccac gcctcgaacg
     1261 tcccgcgctc cagcaactcc acgactctgg cgctgatggc cttgcccacg ctcggcagcg
     1321 tctgcaagcc cttcacgcct tcctctttcg caatcttcgc gagcggcgtc ggccacgtct
     1381 cgatgatctc cgcggcgttg cggtaggagc gcacgcggaa ctggtcgtcg ccccgaatcg
     1441 acatcagcac cgccagccgc tcgaacctcc gcgcgatctc ctcgttcgtc atatgtttga
     1501 aagccgtgat aagtgatgag tgataagtga taagagaaag acggattttc ttatcactca
     1561 tcactcatca cttatcacct ttctcatgcc ttctccagcc ccgcgtactt ctcgataagc
     1621 ttcttctggc cgaagccggg gaagctgacg gtgagcttgg tggagtcgcc gacgccctcg
     1681 cggcgtagga cgaggccgcg gccgtacttc gggtggcgga cgtgcgagcc gggcacgaag
     1741 cccccgccgc cgctggtgga tgcggacgac gctgatgagc cgccgccggt cgcggaggcc
     1801 gcgcgccgct ttggctcggg gacgatgggc ggactggacg cgcccttgaa cttctcgccg
     1861 cgctggcgga agaactcggc gatggaatcg gcgctgtcgt aagtcttgcc ggcgtatttc
     1921 ttctcggccg cgggggcgcc cgtctcgccg cgcagggcgc gggcgacgtg gcgcgactcg
     1981 gcggccgagg agctgcgggc gaaggagagc caggaggggc cgcgcgagag gtcttccatc
     2041 agctcgaagg gcatctcgtt gaggaactgc gaaggctcgc tcgccatctc ctcgccgtag
     2101 acgcggcgct tcatggcgtg cgagacgtag aggaagcgct cggcgcgtgt gatggcgacg
     2161 taagcgaggc ggcgctcttc ttcgagttcg tcggcgtcgg ccgccgagcg cgagtgtggg
     2221 aagagtccgt cctccatacc gacgaggaag acaacgggaa actccagtcc cttcgccgag
     2281 tggatggtca tcagcgtgac gggcgaggcg cgctcgtact gatcctgatc ggagctgagc
     2341 gcggcggcgt cgatgaagtc gcgcaggccg ccgccctcct cgcggtcgta
//
LOCUS       DASVES010000096         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_41626, whole genome shotgun sequence.
ACCESSION   DASVES010000096 DASVES010000000
VERSION     DASVES010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41626"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1214)
                     /locus_tag="VF240_03460"
     CDS             complement(138..1214)
                     /locus_tag="VF240_03460"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX7174324.1"
                     /translation="MIDLRSDTVTKPTEAMRRAMAEAEVGDDVYGEDPTVNRLQERAA
                     EVFDKEAALLVPTGSMGNQIAVKLHTRPGSEVVIEERGHIFNYEMAAMSVVSGALARP
                     VRSADGTGILRWEEIAPAVHGANAAYYVAPTRLLCLENSHNLAGGSVYTRERMEEVCE
                     RAHAAGLFVHLDGARLFNAAVATGETVAALARAADSVMFCLSKGLGAPVGSMLLGTKA
                     FIEEARAWRKLLGGGMRQAGVLAAAGLVALEETPPRLIDDHANARRLAAGAAELPGVG
                     IDPERVVTNIVIFDVSGTPHSAAEITARLRDDHGVLAHGFGDSIRMVTHYDVSADDIE
                     RALDALREVIGSQESGVRSQKVSS"
     gene            complement(1328..2467)
                     /gene="hemW"
                     /locus_tag="VF240_03465"
     CDS             complement(1328..2467)
                     /gene="hemW"
                     /locus_tag="VF240_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7174325.1"
                     /translation="MSQAGIYIHVPFCRSRCSYCDFATGTYESALAAVYVRALAREVA
                     AFDDDAAARAIDTIYFGGGTPSMLTAAQLAHVLEAVHLRFHVLPGAEVTLELNPGTVT
                     PESLRELRALGVNRASFGLQTFDDEELRRLGRTHTADDARRTLHALREAGFRNVSFDL
                     IAGLPEQTLEGWSRNLDEALALRPEHLSLYLLEVHEGTPLADHIRRGFWRAPDPDLAA
                     EMYRLLARRMTGAGYDHYEISNFALPGRESRHNMKYWTGAPVYGFGCSAHSFDGRRTR
                     WSNERDAKRYVALIESGASPVVERTELDEREAFSESLFLALRLLRRGVNLSEHRARFR
                     VDVRADYAADLERFSDAGLVELEDDVLRLTSAGALLSNEVFAAFI"
BASE COUNT          540 a          914 c         1090 g          515 t
ORIGIN      
        1 ccgagcgtcg atttgcccgc gcccgacggg ccgtcgatgg cgatgatgag aggcataaaa
       61 gacagaatac aggagtcagg agtcaggagt cagaatcaaa gcggcggaaa agttgaatgt
      121 tgaatacagc gccgaggtca ggaagaaacc ttctgactcc tgactcctga ctcctgactc
      181 ccgatcactt cgcgtagcgc gtcgagtgcg cgttcgatgt cgtcggccga aacgtcgtag
      241 tgcgtgacca tgcggatcga gtcgccgaag ccgtgggcga ggacgccgtg gtcgtcgcgg
      301 aggcgggcgg tgatctcggc agccgaatgg ggcgtccctg aaacatcgaa gatgacgatg
      361 ttggtgacga cgcgttcggg gtcgatgccg acgccgggaa gctcggccgc gccagcggcg
      421 aggcggcgcg cgttggcgtg atcgtcgatg agacgcggcg gcgtctcttc gagcgcgacg
      481 agtccggcgg cggcgaggac gccggcctgc cgcatcccgc cgccgaggag cttgcgccac
      541 gcgcgcgcct cctcgatgaa cgccttcgtg ccgaggagca tcgagccgac gggcgcgccc
      601 agtcccttcg acagacagaa catcacggag tcggccgcgc gggcgagcgc cgccaccgtc
      661 tcgcccgtgg ccacggccgc gttgaagaga cgcgcgccgt cgaggtggac aaagagtccc
      721 gcggcgtgcg cgcgttcgca gacttcctcc atgcgctcgc gcgtgtagac gctgccgccg
      781 gcgaggttgt gcgagttctc cagacagagc agacgcgtcg gggcgacgta gtaggcggcg
      841 ttcgcgccgt gaaccgccgg ggcgatctct tcccagcgga ggatgcccgt gccgtcggcg
      901 ctgcgcacgg ggcgggcgag cgcgccggag acgaccgaca tcgccgccat ctcgtagttg
      961 aagatgtggc cgcgctcctc gatgacgacc tcggagccgg gccgcgtgtg gagcttgacg
     1021 gcgatctggt tgcccatcga gccggtgggc acgaggagag ccgcctcctt gtcgaagact
     1081 tcagcggcgc gctcctgcaa gcgattgacc gtggggtctt cgccgtacac gtcgtcgccc
     1141 acctcggcct cggccatcgc ccggcgcatc gcctcggtcg gcttcgtcac ggtgtcgctg
     1201 cggagatcaa tcatagggag tcaggagcca gaagtcagga gtcagaatgt gaatgtgtta
     1261 ctcggagtca gggccggacg gcagagagtc tttattctga ctcctgactt ctggctcctg
     1321 actcctctca tatgaatgcc gcgaagacct cgttcgagag cagcgcgccg gcgcttgtca
     1381 ggcggagcac gtcgtcttcg agttcgacga ggccggcgtc gctgaagcgt tcgaggtcgg
     1441 cggcgtagtc ggcgcgcacg tctacacgga agcgcgcgcg gtgctcggag aggttgacgc
     1501 cgcgacgcag gagacgcagg gcgaggaaga gcgattcgga gaaggcttcg cgctcgtcaa
     1561 gctccgtgcg ttcgacgacg gggctggcgc ccgactcgat gagcgcgaca taccgcttgg
     1621 cgtcgcgctc gtttgaccag cgcgtgcggc gtccgtcgaa ggagtgggcc gagcagccga
     1681 agccgtagac gggcgcgccc gtccagtact tcatgttatg acgcgactcc cggcccggta
     1741 gcgcgaagtt tgaaatctcg tagtggtcgt aaccggcccc ggtcatgcgc cgggcgagca
     1801 ggcggtacat ctcggcggcg aggtcggggt cgggcgcgcg ccagaagccg cgtcggatgt
     1861 ggtcggcgag cggcgtgccc tcgtggactt cgaggagata gagcgagagg tgttcgggac
     1921 ggagggcgag cgcctcgtcg agattgcgcg accagccctc tagcgtctgc tcggggaggc
     1981 cggcgatgag atcgaagctg acgtttcgga atcctgcctc gcgcagggca tggagcgtgc
     2041 ggcgcgcgtc gtcggccgtg tgcgtgcggc ccagacggcg caactcctcg tcgtcgaaag
     2101 tctggaggcc gaagctggcg cggttgacgc caagcgcgcg caactctcga agcgactcgg
     2161 gcgtcaccgt gccggggttc agctccagcg tcacctccgc gccggggagg acgtggaagc
     2221 gaaggtgtac ggcctcaagg acgtgtgcga gctgcgcggc cgtgagcatc gagggcgtgc
     2281 cgccgccgaa gtagatggtg tcgattgcgc gcgcggccgc gtcgtcgtcg aaggcggcca
     2341 cctcgcgcgc gagcgcgcgg acgtacactg cggccagcgc gctctcatac gtgcccgtgg
     2401 cgaagtcaca gtaggagcag cgcgaacggc agaacgggac gtggatgtag atgcctgcct
     2461 gactcatcgc ggaagcgcgg attataacac gcggccgccg agacgcgcgg agtgcggtcg
     2521 cgcacggatc ggcccgaaca aacgacgctc ggtgcaggcg gacaactcgt gatgcgccgg
     2581 acggcgtggc attggacacg cgagcgcact tcgcggagtc gtccgcgtgt cgctcgcgaa
     2641 gaggaacgga agcactttct cgtcgcgccg ggctatgtat accagcgcac tttgaagctc
     2701 aaccgatggc gggcgggtgt acgctttcgt tcgcgcaggt gtacggcttt tcgcgtctga
     2761 agggacggct gattggcgcg tgtgcctcaa ctctccatca atccagaagc aattcgcatc
     2821 aaaaaagaaa cttctttata taaaactcgt ggcataacgg ttgatgtagt tactgacgcg
     2881 tcttaatcca tatcccgctc taactaaccg ggtggagacg tgcgccggtt ctccttgttc
     2941 ttctttaccg acttaggcgc acgtgttcat ctaccgacca ctaacaattg aattatggga
     3001 ggagattaac gatgtcagag aaagatcgtg aaggaacggg cggctcaggc ggcggcggc
//
LOCUS       DASVES010000097         5364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_431322, whole genome shotgun sequence.
ACCESSION   DASVES010000097 DASVES010000000
VERSION     DASVES010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431322"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VF240_03470"
     CDS             <1..1098
                     /locus_tag="VF240_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7174326.1"
                     /translation="VSAFFNAAKRAAVPNLARGAEEMLAANSLTASTTAATIAVGSAL
                     GGILATLAGREVVFLLNAATFLASAELIRRIRTRTERKSEVGSQHSAVSSRQWAVGEQ
                     PSSVETNLNQEASLKMETPADTQPTADGPLPSADSLFRRALEDFRGGLRYVRHEPVLR
                     AIFVVAAGWGLGNGVARALYSIFGARLGEAEVAGWGVAARELIERPGEFGISVLFVAM
                     GVGGVLGAPLARRFNNAGGRDLGARLGRSMAFDGCGLALFSLMPSLWVAALVLVAREV
                     NFAVWWTAQQTILMRRADDAYAGRVFASYETLTTLMMVGSMLASGVAADSLGIRPVAL
                     AGGLVITCSGLLWFLLQPQAQVVGVRRGERN"
     gene            1241..2611
                     /locus_tag="VF240_03475"
     CDS             1241..2611
                     /locus_tag="VF240_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7174327.1"
                     /translation="MTTLYCASWLLPITSAPVRDGAVAVRGGQIVGVGTRDEITRRFP
                     AADLNDFGEAAILPGLVNCHSHLELTVMRGYLEREENNFPAWLQKLTIARNEWMTAED
                     RYVSAAWGAVEAARAGVTCVGDASDTAAPCVRALRHAGLRGVVYQEVFGPDARTSGEQ
                     FAKLREKVERERAGESELVTLGVSPHAPYSVSAPLFEAVAQYARAENLPLMVHAAESE
                     AEEQFMREGRGPLAEGLRRRGIEWSAPGVSSIQYLARLGVTGPRTLLAHCVRADDADV
                     ATLAASGTRVAHCPKSNAKFGHGRAPFAAFLRAGVLTGLGSDSVASNNTCDLLEEGRF
                     AVLASRAAGERLADERMVGADEALRAATLAGAWALGFGGLTGALEEGLQADLCVFSLA
                     GAHQTPVHDDPAAALLFASSGRDALLTVVAGREVFRDGRVTTVDEEELRARVKDIARR
                     LGGVTI"
     gene            2658..3593
                     /locus_tag="VF240_03480"
     CDS             2658..3593
                     /locus_tag="VF240_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174328.1"
                     /translation="MTIKKMLVPVLAFVLAVGLALTCATAQQVADPDFKPEIRKPAYA
                     AGRGPVVCLDEAHFNFHTASGRYAPFAELLRRDGYVVNASAAKFSAAALSSCRVLVIA
                     NALGERNQSSWSLPIDPAYTDEEVSAVAAWVKSGGSLFLIVDHMPMPGANRTLAAAFG
                     VRFSNGFALIPDVNAPLVFKRADGLLADHPVTRGATKDERIDAVATFTGSAFRIDSGG
                     EPILVFGPQVISLEPQVAWQFTPETPKTDVRGWLQGATLRHGKGRVAVFGEAAMFTAQ
                     LAGPNKNKVGMNSPDAPQNARLLLNVLGWLTGRLK"
     gene            complement(3937..5244)
                     /locus_tag="VF240_03485"
     CDS             complement(3937..5244)
                     /locus_tag="VF240_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7174329.1"
                     /translation="MKKFLPLFILLLALAPTAPAQTPPTAADLLKSGMEKKEAKDFEG
                     ALADFTRAIELNPNLAEAYLRRGRIHANQGRWNTAMSDITKAVDLDPSLTEALAARAN
                     LRLTRGNDFAGSIADFDKLINLGVELETSYYHRGVAKSRLRDFQGAVADYNAVLELPS
                     RDTETHKFANYKPMAHSARGNARKALGDFDGAIADYSAVLSTALAADPKHPMAAMTYL
                     ERGRAYEAKGEAERARADYDKAIELRPELEGLIKHRDGSASVEAARKKMLEQSDERVK
                     ADPKTAEEFYDRGDNHLMRKEYDAAIADFDKALELNPKLRHVHNTRGSAHMSRGDFQK
                     AIADFTREIESAPQEDMGYNNRGIARQNVGDLAGAIADYTKAIELSPHTAYLYGNRAI
                     ALTLAGRDAEARRDVDKCLQLNPSVREWIESRIKWAEERRARR"
BASE COUNT          824 a         1725 c         1831 g          984 t
ORIGIN      
        1 gtctcagcct tcttcaacgc cgccaagcgc gccgccgtcc cgaacctcgc gcgcggcgcc
       61 gaagaaatgc tcgcggccaa ctcgctcacg gcatcgacga cggcagccac catcgccgtc
      121 ggctccgcac tcggcggcat cctcgccacc ctcgccggcc gcgaggtcgt cttcctactc
      181 aacgccgcca ccttcctcgc ctccgccgaa ctcatccggc gcatccgcac gcggacggaa
      241 aggaagtcgg aagtcggcag tcagcactca gcagtcagta gtcgtcagtg ggcagtgggc
      301 gagcagccgt cgtcagtcga gacgaatttg aatcaggagg cgtctctgaa aatggagacg
      361 ccggccgaca ctcaaccgac tgccgacggc ccgctgcctt ctgccgactc gcttttccgt
      421 cgtgcgttag aagacttccg aggcggtttg cgctatgtga gacacgagcc ggtgctgcgg
      481 gcgatcttcg tggtggcggc cgggtggggt ctgggcaacg gcgtggcgcg ggcgctctat
      541 agcatcttcg gcgcgcgcct cggtgaagcg gaagttgcgg gttggggcgt cgccgcgcgc
      601 gagttgattg agcggccggg cgagttcggc atctccgtgc tgttcgtggc gatgggcgtg
      661 ggcggcgtgc tcggcgctcc gcttgcgcgc cgcttcaaca acgcgggcgg gcgagacttg
      721 ggcgcgcgcc tcgggcgctc gatggcgttc gacggctgcg gcctcgccct cttcagcctg
      781 atgccgagcc tgtgggtcgc ggccctcgtg ctcgtcgcgc gcgaggtaaa cttcgccgtc
      841 tggtggacgg cccagcagac catcctcatg cgccgcgccg acgacgccta cgccggccgc
      901 gtcttcgcct cctacgagac gctgacgacg ctgatgatgg tcggctccat gctcgcctcc
      961 ggcgtcgccg ccgactcact cggcatccgt cccgttgcgc tcgccggcgg ccttgtgatt
     1021 acctgttccg gcctgctctg gtttcttctt cagccgcagg ctcaagtggt gggagtgcgg
     1081 cgcggtgaga gaaattaaca tggatgggca ggatagtcag gatgaagaaa agatgatttc
     1141 agatttgaga tttcaaacct tctcatcctg cccgtcctgc ccatccatgt taatttttgt
     1201 gacttgtcat gccatctgaa cgtaattcgc acctgtcctt atgacaactc tttactgcgc
     1261 gagctggtta ttgccgatca cttccgcgcc cgtgcgcgac ggcgcggtgg ccgtccgcgg
     1321 cgggcagatc gtcggcgtcg gcacgcgcga tgagatcaca cgcaggttcc ccgccgccga
     1381 cctcaacgac ttcggcgagg ccgccatcct gcccggtctc gtcaactgcc actcgcacct
     1441 cgaactgacg gtgatgcgcg gctatctcga acgcgaggag aacaacttcc ccgcatggct
     1501 gcaaaagctc accatcgcgc gcaacgagtg gatgacggcg gaagaccgtt acgtctcggc
     1561 ggcgtggggc gcggtcgagg cggcgcgcgc cggcgtgacg tgcgtgggcg acgcgagcga
     1621 cacggccgcg ccctgtgtgc gcgcgctcag gcacgcgggc ctgcgcggcg ttgtctatca
     1681 ggaggtgttc gggccggatg cgcgcacgtc gggcgagcag ttcgcgaagc tgcgcgagaa
     1741 ggtcgagcgc gagcgcgcgg gagagagcga gctggtgacg ctcggcgtct cgccgcacgc
     1801 gccctactcg gtgagcgcgc cgctgttcga ggccgtggcg caatacgcgc gcgcggagaa
     1861 tttgccgctg atggttcacg cggccgagtc ggaagcggaa gagcagttca tgcgggaggg
     1921 acgcggcccc ctcgccgaag gcttgcggcg gcgcggcatc gagtggagcg cgccgggcgt
     1981 ctcgtcgatt caatatctcg cccggctcgg cgtcacgggg ccgcggactc tactcgcaca
     2041 ctgtgtccgc gccgacgacg cggacgtagc gacgctcgct gcgtcgggca cgcgcgtcgc
     2101 ccactgcccg aagtcgaacg ccaagttcgg acacggccgc gcgcccttcg cggccttcct
     2161 gcgtgcgggc gtgttgacgg gattggggag tgattcggtc gccagcaaca acacgtgcga
     2221 cctgctcgaa gaggggcgct tcgccgtgct cgcctcgcgc gccgccggtg aacgactcgc
     2281 tgacgaaagg atggtcggcg ccgatgaagc cctccgcgcg gcgacgctcg ccggcgcgtg
     2341 ggcgctcggt ttcggcggcc tcacgggcgc gctcgaagag ggccttcagg ccgacctctg
     2401 tgtcttctca ctcgcgggcg cgcaccagac gcccgtccat gacgaccccg cggcggcgct
     2461 gctgttcgcc tcatcgggac gcgacgcgct gctcaccgtc gtcgcgggca gggaagtgtt
     2521 tcgcgacggg cgcgtgacga ccgtggacga ggaagaattg cgcgctcgcg tcaaagatat
     2581 tgcccggcgt cttggaggcg tgacaatctg agaagaattg tttagcagga cgatgaagaa
     2641 tcggaggcga tgtagacgtg acaataaaaa agatgctcgt gccggtgttg gctttcgtgt
     2701 tggccgtggg actcgcgctg acgtgcgcga cggcgcaaca ggtcgccgac cccgatttca
     2761 aacccgagat caggaagccg gcctacgcgg ccgggcgcgg ccctgtcgtc tgtctggatg
     2821 aagcacactt caacttccac acggcctcgg gacgctacgc gccgttcgcc gagttgctgc
     2881 gccgcgacgg ctacgtcgtg aatgcgtcgg cggcgaagtt ttcagccgcc gcgctgtcga
     2941 gttgccgcgt acttgtcatc gcgaacgcgc tcggcgagcg taatcagtcg agctggtcgc
     3001 tgcccatcga cccggcctac acggacgagg aggtgtcggc cgtcgccgcg tgggtgaaga
     3061 gcggcggctc gctcttcctg atcgtcgatc acatgccgat gccgggcgcg aaccgcacgc
     3121 tcgccgcggc cttcggcgtc cgcttcagca acggcttcgc cctcatcccc gacgtgaacg
     3181 cgccgctcgt cttcaagcgc gccgacggtc tgctcgccga ccaccccgtc acgcgcggcg
     3241 cgacgaagga cgagcgcatc gacgcggtcg ccaccttcac cggctcggcc ttccgcatcg
     3301 acagcggtgg cgaacccatc cttgtcttcg ggccgcaagt catttcgctt gagccgcagg
     3361 tggcctggca gttcacgccg gagacgccga agacagatgt gcgcggctgg cttcagggcg
     3421 cgacgctgcg ccacggcaag ggccgggtcg ccgtcttcgg cgaggcggcg atgttcacgg
     3481 cgcagctcgc cgggccgaat aaaaataagg tcgggatgaa ttcgcccgac gccccgcaga
     3541 acgcgcggct cctgctcaac gtgctgggct ggctcacggg caggctgaaa taaggatgaa
     3601 ggcggaagga tgaaggatga agtgacgagg ccgcgcgggc agcggcggca gagttacgag
     3661 ccgccggagg aggcggcaga atgtagccac gggcgtgagc ccgtggtagc ggcaaggaaa
     3721 ataaacaagc cccggagggg cgaaagaaaa tcaacgtgtg cgtgacgccg gttgattttc
     3781 tttcgcccct ccggggcttc ccactttctt ctcaacggct gacccacggc tcacgccgtg
     3841 ggctaaaatc tgccgccgcc tccggcggct ggttgacggc tgcgacccgg ccggcgcggc
     3901 tcttcaactt ttgccttttg cctttttact tttgccttac ctgcgggcgc gtcgctcctc
     3961 ggcccacttg atgcggctct cgatccattc gcgcacggag gggttgagct gcaaacactt
     4021 gtccacgtcg cggcgggcct cggcgtcgcg gcccgcgagc gtgagggcga tggcgcggtt
     4081 cccgtagagg taagccgtgt gcggcgacaa ctcgatggcc ttcgtgtaat cggcgatggc
     4141 gcccgcgagg tcgcccacgt tctgacgggc gatgccgcgg ttgttgtagc ccatgtcctc
     4201 ttgcggggcc gattcgatct cacgggtaaa gtccgcgatg gccttctgga agtcgccccg
     4261 gctcatgtgg gcgctgcccc gggtgttgtg aacgtgacgc aacttcgggt tgagttcgag
     4321 ggccttgtca aagtcggcga tggccgcgtc gtactctttc ctcatcaagt gattgtcgcc
     4381 gcggtcgtag aattcttcgg ccgtcttcgg gtcggccttc acacgctcgt ccgactgttc
     4441 cagcatcttc ttccgcgccg cctcgacgga tgcgctgccg tcgcggtgct taatcaaacc
     4501 ctccagctcc gggcgcagct cgatggcctt gtcgtaatcg gcccgcgcgc gctcggcctc
     4561 gcccttcgcc tcgtaggcgc gcccgcgctc aagataggtc atggcggcca tcgggtgctt
     4621 cgggtcggcc gcgagggcgg tgctgaggac ggcgctgtag tcggctatcg ccccgtcgaa
     4681 gtcgccgagc gccttgcggg cgttgccgcg cgcgctgtgg gccatcggct tgtagttggc
     4741 gaacttgtgg gtctcggtgt cgcgcgacgg gagttcgagg acggcgttgt aatcggcgac
     4801 ggcgccctga aagtcgcgca ggcgcgactt cgccacgccc cggtggtagt acgaggtttc
     4861 gagttccaca ccgagattaa tcaacttgtc gaagtcggcg attgagccgg cgaagtcgtt
     4921 gccgcgcgtc aggcgaaggt ttgcgcgcgc cgccagtgct tcggtcagag aggggtcgag
     4981 atcgactgct ttggtgatgt cagacatcgc cgtgttccat ctgccctgat tggcgtggat
     5041 gcgcccgcgc cggagatatg cttccgccag attcggattc aactcgatag cgcgcgtgaa
     5101 gtcggcaagc gcgccctcga agtctttggc ttccttcttc tccatccccg atttgagtaa
     5161 gtcagcggcg gtcggcggcg tctgcgccgg agcggtggga gcgagggcga gaagcaggat
     5221 gaagagcgga aggaattttt tcacgacggc ctccgtcagc ctcggcgggg aattgaactg
     5281 accgacggcc cgaacttaaa ggcgcggcgg cagccttgtc aatgctgaca ggacagtacc
     5341 gcgaggcttc cgctcgcggt actg
//
LOCUS       DASVES010000098         6127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1401269, whole genome shotgun sequence.
ACCESSION   DASVES010000098 DASVES010000000
VERSION     DASVES010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401269"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VF240_03490"
     CDS             complement(<1..324)
                     /locus_tag="VF240_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174330.1"
                     /translation="MNAIIIMLALALTITVAFFTGGDGAAALFLCLVLAAPCGALVHR
                     AGGEFLLRVFVLGVLVRVAVGTLIFHAGMQEFFGGDALTYDFQGTEVYRYWTGQIQYR
                     EIRFDL"
     gene            complement(480..1934)
                     /locus_tag="VF240_03495"
     CDS             complement(480..1934)
                     /locus_tag="VF240_03495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7174331.1"
                     /translation="MKSLLATAPLVLGIAIALLLPEHGPSAVLVCTTLALVACLLLSR
                     VGAADKNFLLQIFVAGLLARVLVGAAIFTSGLQVFFGGDAVLYDEVGDALAQVWHGQM
                     HAASFMSEAGRFMTRDSGMSYLVGGIYFVTGRNMLAVQFASAVLGAATAPLIYLCAHH
                     IFQNVRVARLSAVFVAFYPSLVLWSSQGLKDGPVVFLLALSMLATLRLSERMSVSQLL
                     VLVASLFGIHTMRFYIFYMVLAATCGAFVIGRSTLTARSVARQTVVVVALGMAMTYLG
                     VLRTATTQLETFGNLELMQQSREDMARTADSGFGRDVDVSTTSGALTAIPVGMVYLLF
                     APFPWQLTNVRQSITLPEMLVWWTSFPLLVVGIWFTLKYRARQALPILIFTTMLTLAY
                     SVVQGNVGTAYRQRSQLLVFYFIFASVGFILLRERGEDRRRRLAQEAERGRGWVRARA
                     PGSPVASRPSARGLVGAARPAGGRDLKVGAAGEG"
     gene            complement(2146..2961)
                     /locus_tag="VF240_03500"
     CDS             complement(2146..2961)
                     /locus_tag="VF240_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174332.1"
                     /translation="MSALDDDARPGGPSLTPAEAPVRVATAERRHRPGKLRLAALAAV
                     AVLPSFLKRPCYRLFFGYKIGRRVSIGLSIIDAGECVIADDVRIGHLNLIVGVGRVEM
                     GDHVKIGHLNVIRGGWEVRLGRWAEIIRLNEINSIPDPDVVNPIEPVFRLGPASVVTT
                     GHKIDFTDRVEIGRRSILGGRNSSLWTHNRQRTRPVIVGDYAYIGSEIRVAPGGVIPS
                     RCIVGIGSVITGRIEDDNHLIAGVPAKAVKPLSQEDQFLVEYKTRPDLPDDVV"
     gene            complement(3006..4079)
                     /locus_tag="VF240_03505"
     CDS             complement(3006..4079)
                     /locus_tag="VF240_03505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7174333.1"
                     /translation="MEVLTLIPNRYGFAPGQRGSIELWERVLGPAGINLHWAPFESER
                     LREILYQPGRRVEKAREMLRGYSERLRLLGRLKEFDAVFVYREAALLGPAFIERQVAR
                     RGLPIIYQLDDPLFVPYRSPSNGLFSYLKFFSKIAEIIRLSKVVIVNSSHIREFAERY
                     NQNIWQVPSIVDTEQYVYRPQAANVERVTVGWSGSPTTMKNIRVVADALRELRGRVPH
                     DVHLIGGEEFDLPGVEYTAQKWRADTEVEDLRRMQVGMVPLPVNEWNKYKFYMKTAQY
                     MALGIPPVCTPMGSNPEVIRHGETGFLADTTEEWVTYLERLIRDTPLRLEVSERAARE
                     ARAKYSLEANAEKVIDAFRSALK"
     gene            complement(4106..4948)
                     /locus_tag="VF240_03510"
     CDS             complement(4106..4948)
                     /locus_tag="VF240_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7174334.1"
                     /translation="MGANSAARRLVKKALYPVLNEHTYRYIQAASKAWDIRSGKWTEP
                     EIELLPLAVRAGDTVLDIGANFGLYCHHLSRVIGAGRVYAFEPVPFTFSTLKLIARLL
                     RFRPGVELVEKGCSDHTGALTFTIPVQASGAVAAGQAYIGARDDEREGKEGQVRWEGT
                     RDVTCEVVALDEFLPRVERLSLIKCDVEGAELLAFRGAARLIGEHLPTVICEINPWFL
                     EGFNIRLEELLGFFFERGYKLYYYRKGEGHGRLREVRAEDVVEDNYVFVHPSRIDRLA
                     AVLG"
     gene            complement(5081..5320)
                     /locus_tag="VF240_03515"
     CDS             complement(5081..5320)
                     /locus_tag="VF240_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174335.1"
                     /translation="MEAENTVELRFAICVKNDGHEDDLKVRTVYQILPDESAARSDYV
                     RVIDETGEDYLYPAAYFVPVEVTPEAARTLVLAAS"
     gene            complement(5325..5564)
                     /locus_tag="VF240_03520"
     CDS             complement(5325..5564)
                     /locus_tag="VF240_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174336.1"
                     /translation="MAVHFQIEGGITDVETIAAGSSIRELPELRARFGRGRWRKLKGV
                     ANVRLSDGTIRLAEVHWYEAHGIGKVRMKIKQYLD"
     gene            complement(5590..>6127)
                     /locus_tag="VF240_03525"
     CDS             complement(5590..>6127)
                     /locus_tag="VF240_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174337.1"
                     /translation="AYVAHLPFYPPETTVTLALWSSRLPVTWKDRVKRVPLVQKNSAA
                     LKRLARRAGLTRQLELKVVEYFDFYPTPAGFCGIRERTEFERGPVEDYLYSLFHVIQA
                     TGNDQLAPVVAERLGAGEAVDNPELVRRLNDDLRHGRPIEGRLSPTHYGVERANFTRD
                     EVESALAAQGAKAAPASV"
BASE COUNT         1050 a         2002 c         2083 g          992 t
ORIGIN      
        1 caggtcgaac ctgatctccc tgtattggat ctgccccgtc cagtagcgat agacctccgt
       61 gccctgaaag tcgtaagtca gcgcgtcgcc gccgaagaac tcctgcatcc cggcgtggaa
      121 gatgagcgtc ccgacggcga cccggacgag gacgccgagg acgaagacgc gcagcaggaa
      181 ctccccgccc gcgcgatgca ccagcgcccc gcacggcgcc gccaggacga gacacaaaaa
      241 gagcgccgcc gccccgtcgc cccctgtgaa gaaagcgacg gtgatggtca gcgccagcgc
      301 cagcatgatg attattgcgt tcatcttgaa gagaagcgtt ggtgtctgca ccgccgccgg
      361 gcagtcaagt tgtaacgcca catagtgtgc ggcgcagccg acgcggcgcg caacccgccg
      421 acgcggcgca acttcgcgcg cgcgtgtctc accacgcggc ggcgatggcg ttcgccgtcc
      481 tacccctcgc ccgccgcgcc gactttcagg tcacgcccac ccgccggccg ggccgcgccg
      541 acgaggcccc gcgcgctcgg tcgcgacgcg acgggcgaac ccggcgcgcg agcgcgcacc
      601 catccccggc cccgctcggc ctcctgcgcc agccgccggc ggcggtcttc cccacgctcc
      661 ctcaacagga tgaaccctac cgaggcgaag atgaagtaga agacgagcag ttgtgagcgc
      721 tgccgatagg ccgtccccac gttgccctgc acgaccgagt aggccagcgt cagcatcgtc
      781 gtgaagatca ggatggggag cgcctgccgc gcgcggtact tgagggtgaa ccagattccg
      841 accaccaaca gcgggaacga ggtccaccag accagcatct cgggcagcgt gatggactgc
      901 cggacgttgg tgagctgcca cgggaacggg gcgaaaagca ggtagaccat ccccacgggg
      961 atggccgtga gcgcgcccga ggtggtggac acgtccacgt ccctgccgaa gccggagtcg
     1021 gcggtgcgcg ccatgtcttc ccggctctgc tgcatcaact cgaggttgcc gaaggtctct
     1081 aactgcgtgg tcgccgtgcg gagcacgccg agataggtca tcgccatccc gagggcgacg
     1141 accaccaccg tctggcgcgc gacgctgcgc gccgtcagcg tcgatctccc gatgacgaac
     1201 gccccacagg tggcggccag caccatgtag aagatgtaga agcgcatggt gtgaatgccg
     1261 aacagggacg cgaccagcac cagcagttgc gacacgctca tccgctcact tagccggagc
     1321 gtggccagca tcgacagcgc cagcaggaag acgaccggcc cgtccttgag tccctgcgac
     1381 gaccacagca ccagcgacgg atagaaggcc acgaagacgg ccgacagccg cgccaccctc
     1441 acgttctgga agatgtggtg cgcgcacagg tagatgagcg gggccgtcgc cgcccccagc
     1501 acggcgctgg cgaactgcac ggccagcatg ttgcgccccg tcacgaagta gattccgccg
     1561 acgaggtagg acatgccgct gtcccgcgtc atgaatcgcc cggcctcgga catgaacgac
     1621 gcggcgtgca tctgcccgtg ccagacctgc gcgagcgcgt ccccgacttc gtcgtagagc
     1681 acggcgtcgc cgccgaagaa cacttgcagc cccgaagtga agatggcggc gccgacgagc
     1741 acgcgcgcca gcaggccggc gacgaagatt tgcaggagga agtttttgtc ggcagcgccg
     1801 acacgcgaga gcaggagaca ggcgaccagg gcgagggtcg tgcagacgag cacggccgac
     1861 gggccatgct caggaaggag gagagcgatg gcgattccca gcacgagcgg cgccgtcgcc
     1921 agcagggatt tcatagctag ggaactctga ctttcttcgt gcgaggcgcg cgacgccggg
     1981 cgcggcgcgc cggcgcagtt caacgcgagc cgtggtcact cgtgggtcgc ttcgccggtc
     2041 ttcaaaaata gggaaaggcg tgaacaggcg agggcgcgcg gcggcgtcgc cgatccgagg
     2101 gacggccgcg gtggccacgg cggccggcgc tgctcgcttc taacgtcaga cgacgtcgtc
     2161 gggcaggtcg ggccgcgtct tgtactcgac gaggaattga tcttcctggc tgagcggctt
     2221 gacggccttc gccgggacgc ccgcgatgag gtggttatca tcttctatcc ggcccgtgat
     2281 gaccgacccg atgccgacga tgcagcggga tggtatcacg ccccccggcg cgacgcgaat
     2341 ctccgagccg atgtaggcgt agtcgccaac gatgacgggt cgcgtccgct gccggttgtg
     2401 cgtccacaag ctggagttgc gcccgccgag gatgctgcgg cggccgatct cgacgcggtc
     2461 ggtgaagtcg attttgtggc cggtggtgac gacgctggcc gggccgagac ggaagaccgg
     2521 ctcgatgggg ttgaccacgt ccgggtcggg gatggagttg atctcgttga ggcggatgat
     2581 ctcggcccaa cgcccgagac gcacctccca cccgccgcgg atgacgttca ggtggccgat
     2641 cttcacatgg tcgcccatct cgacgcggcc cacgccgacg atcaaattca gatggccgat
     2701 ccgcacgtcg tccgcgatca cgcactcgcc cgcgtcgatg atgctcagac cgatggagac
     2761 gcgcctgccg attttgtatc cgaagaagag tcggtagcac ggacgcttca ggaagctcgg
     2821 cagcacggcc acggcggcga gcgcggcgag gcggagctta ccgggccggt gccggcgctc
     2881 ggcagtcgcc acgcggacgg gcgcctcggc cggggtgaga ctcgggccgc ccggacgcgc
     2941 gtcgtcatca agcgcgctca tcgggcgcgt tcgcgcttcc gtgccttctg cctctcgcgg
     3001 ggtcgtcatt tcagtgcgga gcggaacgcg tcgatgacct tctcggcgtt ggcttcgagg
     3061 ctgtacttgg cgcgcgcctc gcgggcggcg cgttcggaca cctccagccg cagcggcgtg
     3121 tcgcgaatca gacgttcgag gtacgtcacc cactcctcgg tcgtgtcggc cagaaagccc
     3181 gtctcgccgt ggcggatgac ctcggggttc gaccccatcg gcgtgcagac gggtgggatg
     3241 ccgagggcca tgtactgcgc cgtcttcatg tagaacttat acttgttcca ctcgttgacg
     3301 ggcagcggca ccatcccgac ctgcatccgg cgcaggtctt ccacctccgt gtcggcccgc
     3361 catttctgcg ccgtgtactc gacgcccggc aggtcgaact cttcgccgcc gatgagatgc
     3421 acgtcgtgag gaacgcggcc acgcagttcg cgcaacgcgt cggcgacgac ccggatgttc
     3481 ttcatcgtcg tcgggctgcc gctccagccg accgtgacgc gctccacgtt cgccgcttgc
     3541 ggccggtaga cgtactgctc ggtgtccacg atgctcggca cctgccagat gttctggttg
     3601 tagcgctcag cgaactctct gatgtgcgac gagttgacga tgacgacctt gctcaggcgg
     3661 atgatctcgg caatcttgct gaagaacttc agataggaga acagcccgtt cgaggggctg
     3721 cggtagggca caaaaagcgg gtcgtcgagt tggtagatga tggggagtcc gcgccgcgcc
     3781 acttgccgct cgatgaaggc ggggcctaag agcgccgctt cgcggtagac gaagacggcg
     3841 tcgaattcct tgaggcggcc gaggaggcgc aggcgctcac tgtagccgcg cagcatctcg
     3901 cgcgccttct cgacgcggcg gccgggctgg tagaggattt cgcgcagccg ctccgactcg
     3961 aacggcgccc agtgcaggtt gatgccggcg ggtcccagca cgcgctccca cagctcgatg
     4021 ctgccgcgct gaccgggcgc gaagccgtag cggttcggga tgagggtcag gacttccatg
     4081 cggggaggat tctaaccggg aaccactacc cgagcacagc ggcgagacgg tcaatgcgcg
     4141 aagggtggac gaagacgtag ttgtcctcga ccacgtcctc ggcccgcacc tcgcgcaagc
     4201 gcccgtggcc ttcgcctttg cggtagtaat agagcttgta gccgcgctcg aagaagaacc
     4261 cgagcagttc ttcgagccgg atgttaaagc cttcgaggaa ccacgggttg atctcgcaga
     4321 tgacggtggg gaggtgctcg ccgatcaggc gcgcggcgcc gcggaaggcc agcagctccg
     4381 ccccctccac gtcgcacttg atgagcgaga gccgctcgac gcgcggcaga aactcgtcga
     4441 gcgcgacgac ctcgcaggtc acgtcgcgcg tcccctccca gcgcacctgc ccctccttgc
     4501 cctcgcgctc gtcgtcgcga gcgccgatgt aggcctgccc ggcggccacc gcgcccgacg
     4561 cctgcacggg gatggtgaac gtgagcgcgc ccgtgtggtc gctgcaaccc ttttcgacga
     4621 gttcgacgcc gggacggaag cgcagcaggc gcgcgatcag cttgagggtg ctgaaggtga
     4681 acggcaccgg ctcgaaggcg tagacgcggc ccgcgccgat gacgcgtgag aggtgatggc
     4741 agtagaggcc gaagttggcg ccgatgtcga ggacggtgtc gcccgcgcgc acggccagcg
     4801 ggaggagttc gatctccggc tccgtccact tgcccgagcg gatgtcccaa gccttcgagg
     4861 ccgcctggat gtaccgatac gtgtgctcgt tcagcaccgg gtagagcgcc ttcttaacca
     4921 gacgccgcgc ggcggagtta gcgcccatcg ttcatacctc ggatgtaccc ggccgcccct
     4981 tgtcagtggc gcacgcttcg cgccgggatg agttggtgtt ggaggacatc tggttgcggg
     5041 gttcgattga gagaaaatat tcggcggcgg cgaggaagag tcaggaggcc gcgagcacca
     5101 gcgtccgggc cgcctcgggc gtcacttcaa cggggacgaa gtacgcggcc gggtacagat
     5161 aatcctcgcc cgtctcgtct atcacacgga cgtagtcact tcgcgccgcc gactcgtcgg
     5221 ggagaatttg gtagacggtt ctcaccttca ggtcgtcttc gtggccgtcg tttttgacgc
     5281 aaatggcgaa acgcagttct acagtgttct cggcttccat acttctaatc caaatactgc
     5341 ttgatcttca tcctcacttt gccgatgccg tgcgcctcgt accaatgtac ttcagccaga
     5401 cgtatcgtac catcggagag gcggacattg gcaacgccct ttagttttcg ccagcgccct
     5461 cggccgaatc gcgcccgcaa ctcgggcaac tcccgtatcg agctaccggc ggcaatcgtc
     5521 tccacgtcgg tgatcccgcc ctctatctgg aagtgtacgg ccacggccag aagattatca
     5581 gtgtgaagtt cagaccgacg ccggcgccgc cttcgcgccc tgcgcggcca gcgcgctctc
     5641 gacctcgtcg cgggtgaagt tggcgcgctc gacgccgtag tgcgtgggcg agagccggcc
     5701 ctcgatgggg cgcccgtggc gcaggtcgtc gttgagccgg cggacgagtt cggggttatc
     5761 cacggcctcg cccgcgccca ggcgttcggc aacgacgggc gcgagttggt cgttgccggt
     5821 ggcctgaatg acgtggaaga ggctgtagag gtagtcctcg acggggccgc gctcgaactc
     5881 cgtgcgctcg cggatgccgc agaagccggc gggcgtcggg tagaagtcga aatactcgac
     5941 gaccttcagt tccagttggc gcgtgaggcc ggcgcggcgc gcgaggcgtt tgagagcggc
     6001 gctgttcttc tgtacgaggg gcacgcgctt gacgcggtct ttccacgtca cgggcaggcg
     6061 gctcgaccag agcgccagcg tgacggtcgt ctccggcggg tagaacggca ggtgcgcgac
     6121 gtaggcg
//
LOCUS       DASVES010000099         6242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_608089, whole genome shotgun sequence.
ACCESSION   DASVES010000099 DASVES010000000
VERSION     DASVES010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608089"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VF240_03530"
     CDS             complement(<1..856)
                     /locus_tag="VF240_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX7174338.1"
                     /translation="MNKLDAALSPIRITGMTVHVTRIPVLIKRRHGSGDVEGSVRNAI
                     LRLDTDAGITGWGDAAPWAVFTGTIEAAVAALHVYLRPVLIGADASRIGALMEAADHA
                     LVGHPEAKAAIEMALFDIVGQAAGLPVAELLGGRCRDDIALSFSVADPDFDRDIDTVK
                     RLYGEGLRLFKMKTGFAGHAQDLRRMERFRKEIPADAELRIDYNQGMTAHEAIRQLRD
                     VEAFEPAFIEQPVPGHQRAALGEIARALDTPVLADESVFTPSDALTVAAGRLADLVSI
                     KIMKHGGMM"
     gene            1338..2387
                     /locus_tag="VF240_03535"
     CDS             1338..2387
                     /locus_tag="VF240_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7174339.1"
                     /translation="MGANRADPGKVGGTDIHPNRRQPVTGLRLPARDEVTVQFAHSAY
                     RLAERFEARGTGVRHFQTWTLDDTLARVGEADVLVISGFWRNEMQERAKRLAYIQSIG
                     AGYDQFPLDDLRARGIPLANASGVNRDAVAQHGMALILALARHLPQARDNQRARVWRG
                     MISSLAEREDELSGHTLVVLGLGAIGSQLARIAKACGMLVIGLKRDTAKHDGVADEVL
                     PPDRLLDVLPRADFVALCCPLTAATRSIIDAAALAAMRPSCYLVNIARGGCVDEPALL
                     EALRRKGIAGAAIDHFWDEPLPPESPFWDLDNVFITPHTGGETRKYEDRVTGVVAENL
                     ERLWAGRDDLENRIV"
     gene            2466..3095
                     /locus_tag="VF240_03540"
     CDS             2466..3095
                     /locus_tag="VF240_03540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7174340.1"
                     /translation="MGGDPKVRAAVDANSLRASRARSRPRWRGPSIVRAQGRDRRSNR
                     LADQPSGGRADANVAAKPASRLGIRDQIDLHRGDIDEASRCLSEADVAFNPRARCPGI
                     PQKLLNYMAARKAVVAFAGSAKVLKHCESGLVVPDNDIEAFAAACVRLVDDEHLAEAL
                     GDNARQHVEHQCTWERAGEATEAVYRRLLRAYRKRAHATGRSHHSSVHG"
     gene            5326..5691
                     /locus_tag="VF240_03545"
     CDS             5326..5691
                     /locus_tag="VF240_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174341.1"
                     /translation="MGLAIETTTGSYAFTARTAEDFKIPFLGPHFSVGAAVELRYIGG
                     DEARYASRVDAHLTWRSIAPIASYAFDGDKTSWIISWGGQTAVYDDQTKVATFALSGL
                     TVGISLRCSSPGRPAADSA"
BASE COUNT         1074 a         2050 c         2077 g         1041 t
ORIGIN      
        1 ccatcatgcc gccgtgcttc atgatcttga tgctcacgag gtcggccagg cgccccgccg
       61 cgacggtcag ggcgtccgac ggcgtgaaca cgctctcgtc ggcaagcacg ggggtgtcga
      121 gcgcccgcgc gatctcgccc aaggcggcgc gctggtggcc cggcacgggc tgctcgatga
      181 aggccggctc gaacgcctcg acgtcgcgga gctggcggat cgcctcatgt gcggtcatgc
      241 cctgattgta gtcgatgcgc agttcggcgt cggcggggat ctccttgcgg aaccgctcca
      301 tgcgcctcag gtcctgggcg tggccggcga acccggtctt catcttgaag aggcgcaggc
      361 cctcgccgta gaggcgcttc accgtgtcga tgtcgcggtc gaagtcggga tcggcgacgg
      421 agaaagacag cgcgatgtcg tcgcggcacc gcccgccgag cagctcggcc acgggcagcc
      481 ctgccgcttg gccgacgatg tcgaacagcg ccatctcaat cgccgccttg gcctcgggat
      541 gaccgaccaa ggcgtggtcg gcggcctcca tcagcgcccc tatgcgggag gcgtcggcgc
      601 cgatcaggac cggccgcagg tagacgtgga gcgccgcgac ggcggcttcg atcgtgccgg
      661 tgaagaccgc ccacggcgcg gcgtcgcccc aaccagtgat gccggcgtcc gtgtcgagcc
      721 ggagaatcgc gttcctgacc gagccctcca cgtcgcccga gccgtgccgg cgcttgatca
      781 gtacggggat gcgggtgacg tggacggtca tgccggtgat gcggatggga gaaagcgcgg
      841 cgtcaagctt gttcaaggga tggcctcatc tggttgagtg tttcggaagt gggtgcacgt
      901 ccgccgagtt cggcggcgca tgaggatccc ccgcgttatc tatacggtgg tgccggggct
      961 caatcgagat aacgggccga gttgttgggc cgacagaact agttgatcat cgtgtcgatg
     1021 tcgaacgcgg aaggccagag acctcccgca gatgccggct gaaggggcac atgttggtgc
     1081 cttcgagaag tattgcaccc gcgtttcgcg catgcgtcca tgaacgcctg ggccatctcg
     1141 accgtctcgg cgacgcgaac atcggggtca agctccagag cgttaccgat gtatggaact
     1201 gcggaacgac ggcacgacct catgccgttg atcgtgtccc ggaatatccc ttgtcgatca
     1261 cccgacccgt tgaagtggcg agatctgtcg ccgagcgccg gttcgggttg accagggccc
     1321 cgcggagggg cagcgtcttg ggcgcgaacc gagccgatcc gggaaaggtc ggcggaacgg
     1381 acatccatcc aaacaggaga cagccggtga cagggcttag actccccgcc cgcgacgaag
     1441 tcacggtcca gttcgcgcac tccgcctacc gcttggcgga gcggttcgag gcgcggggca
     1501 ccggcgtgcg ccacttccag acctggactc tggacgacac gctggcgcga gtgggcgagg
     1561 cggacgtcct ggtcatctcg ggcttctggc gcaacgagat gcaggagcgc gcgaagaggc
     1621 tggcctatat ccagtcgatc ggcgcgggct acgaccagtt cccccttgac gacctgcgcg
     1681 cgcggggcat cccgctggca aacgctagcg gcgtcaaccg ggacgccgtt gcccagcacg
     1741 gcatggcgct catcctcgcc ctggcgcgcc acctgcccca ggcacgggac aatcagcgcg
     1801 cccgcgtctg gcggggcatg atctcctcgc tcgccgagcg cgaagacgaa ctttctggcc
     1861 acaccctcgt cgtcctgggc cttggcgcca tcggctccca gctcgcccgg attgcgaagg
     1921 cgtgcggcat gctggtgatc ggcctgaaac gcgacacggc gaagcacgac ggcgtggccg
     1981 acgaggtgct cccgcccgac cggctgctcg acgtgcttcc ccgcgctgac ttcgtcgccc
     2041 tctgctgccc gctcacggcg gccacccgca gcatcatcga cgcggccgcc ttggcggcga
     2101 tgaggccctc ctgctacttg gtcaacatag cgcggggcgg ctgtgtcgac gagccggcgc
     2161 tgctggaggc gctccgcagg aaggggatcg ccggcgccgc gatcgaccac ttctgggacg
     2221 agccgctgcc gcccgagtcg ccgttctggg acctcgacaa cgtgttcatt actccgcaca
     2281 ccggcggcga gacacgcaag tacgaggacc gcgtaactgg tgtcgtggcc gagaacctgg
     2341 agcggctctg ggcgggccgg gacgacctcg agaaccggat cgtgtaggcg cgcccggggg
     2401 cgtcagcggg cgccgacgcc ccccaggtat cgccaggaga ataatccacc accggagggc
     2461 ctccaatggg gggcgacccc aaggttcggg ccgcggtgga cgccaactcc ctccgggcca
     2521 gccgggccag atcgcggccg agatggcgcg ggccatcgat cgtgcgggct caagggcgtg
     2581 accgacgatc caatcgtctg gcagaccagc catccggtgg gcgcgccgac gccaacgtcg
     2641 cagcaaaacc ggcatcccgc ctcggcattc gcgaccagat cgatcttcac cggggcgaca
     2701 tcgatgaagc ctcaaggtgt cttagcgagg cggacgttgc tttcaaccca cgcgcgcgct
     2761 gtccgggtat cccgcaaaag ctgctcaact acatggccgc acgcaaggct gtggtagcat
     2821 tcgcggggtc tgccaaagtg ctcaagcact gcgagagcgg cctcgtcgtg cccgacaacg
     2881 acatcgaggc ctttgctgcg gcctgcgtcc ggctggtcga tgacgagcat ctggcggagg
     2941 cgctcggcga caatgcccgc cagcatgtgg agcatcagtg cacctgggag cgcgccggtg
     3001 aggcgaccga agccgtctac aggcgtctgc tgcgagcgta tcgtaaacgc gcccacgcga
     3061 ccgggcgatc gcaccatagc agtgttcatg gctagcttag cttgcctttg cagccaaatg
     3121 ccgttgcggt tctctaatcg cggttcatgt tcgactgtcg acacccgctc aggttccggg
     3181 gctactccgc cgaacggctc atgcgcatgc tgaccgcctt cgattgcgag gtcagcatcg
     3241 tggtcgtgga gaagggccgc gaggctgcta ccccgtgcgc gctgcggtca caatcgacaa
     3301 gcagtgacgt gcttgaatgc aagccccgac cccggagcat cggttgccga tcgaggtcag
     3361 ggcgccgagc tggaacgaag tcgccgagag cctccggccg cggtgatgcc acggcaatgc
     3421 cacggcatag ggcaaatcct gcgccgtcag caggttagct ctatctagcg gtcgctacga
     3481 tttcgcttca gcggtcgcta ggtttaagct aacatgaggc cttgccaagc aggtcctacc
     3541 aagcaggtcc tacagggtga tcctgtcaat gtcgcgtggg tttccatggt taggcgcctt
     3601 gccgacctca gcctgccggc gagccgcgat cgccgccgcc tagaccagct cctgggacac
     3661 gaccgactaa gacgcccatc gtcatcacgc ggaagccagg aatgccgctc ccgtaccgtg
     3721 atgagccgta gaacggcgca aacgaaaggg cagcgatgat gtccttcacg caaagcccac
     3781 atcagccgcg gctgtatgtt gccgatccct ccgaagacga ggcggggtgg atcggctagg
     3841 tgttgccaga gggcagcaag ggaccgaaaa cgacagggct ggccgatgcc ctgcagtccg
     3901 aatgcctgcg cggcagcttc atcttcctct cggtcgaccc gggatggcac gcgatcgcgc
     3961 ccgccgcggc tctgatcgac gtcgtcgaca gcctgatgcg cggtcggcaa cccggccgcc
     4021 ttcccatctg gatcaaggat gccgcggcga cgccggctga ggccgccggg acggcggcac
     4081 ttgcacggat cgccgatgcc ggatccggca tcaccgacgg cttcagggct gacctcgtcg
     4141 gcgacgtgct ccgtctggta gtgggaccga cccccgatct ggcgctggcg agcgacagga
     4201 cggctctcgc gatcggtaag ggccaccccc gcgaaatcct atttcagggc acccagaagc
     4261 cgaagagccg cgccggcggc acgagccttc tcttcgaagg ccgcgcaccg ttctatcccc
     4321 gaagccgggc ggtgattgcc gcgacccgac cgccgtcgcg ccgccgagcg cgacgcgacg
     4381 ggcgggcgcc catgcgcgga gtcttacgga gtcttacaac gcggcgaccc cgaccggcct
     4441 cgtcgttact gtcggtgccg acggcgggtg gcagaagctg ctgctggggc agaccgccgg
     4501 cgaggccaac atccagttcg gcttcgatct tccgcggccc tcggtgcagg cggtgctcca
     4561 gacgagcgac ctttcgtggt cgtcgccaat gcaacggagc tcggcagcct cgccggctat
     4621 ccgccggagc cggcagccgc gggcgagccc cggttccaca acactatgac gttcgacggc
     4681 tgagcactcg ccgccgacgt gggacaggcc aacgacgtac cggcgactac tatagatcag
     4741 acgtcacgtc ttcgacgcag ggagacatat tttaagaagc ttgtcgaagt tacaaaaaca
     4801 aggtcagctt cgccgtctta gatttcagaa tgctcaggac cttctcactc tcaaggcgtt
     4861 tatgctgcac tggttcattg agcgcaaaga ccgcgtcttc ttgctgcagc ttgggggcgt
     4921 gcctccggag gtttttggct acgtccttag ccgttgcaca ctgccgccgg tattcgaggg
     4981 ctacaacacc gcgaacctcg ccctcaacat cggagacggt ttcctacagc tgatgcggcc
     5041 gacgctcaag gaaagcgacg atatctatcc aagcattccc gccggggcca gagcgggaaa
     5101 ggaacagcag gagctcaagc ggcatgtgaa tttaatcatg tcaggcggta ctatcataat
     5161 ccgcgccaca gaaatgcggg ctagacctgc tgctggtggc gacccggtcg gaagaagcag
     5221 gctggcggtt cgaggcccca ccagacgacc ggcacgctgc cgctcggcaa gctggccgag
     5281 cgttacctgg gatggaagga ggcaccggat atcgagatcg ccggcttggg tctcgccatc
     5341 gagaccacga ccggctccta tgccttcacc gccaggacgg ccgaggactt caagatcccg
     5401 ttcctcggcc cgcactttag cgtcggcgct gccgtcgaac tccgctacat cggcggcgac
     5461 gaagcccgct acgccagccg tgtcgacgcg catctcacct ggcggtcgat cgcccccatc
     5521 gccagctacg cgttcgatgg cgacaagacg tcctggatca tttcatgggg cggccagact
     5581 gcggtctacg acgaccagac caaggtcgcg accttcgcgc tcagcgggct caccgtcggg
     5641 atctcgttgc gatgttcgtc tcctgggcga ccggcagccg attcggcctg accgcgccgt
     5701 ggaacgtact cgacgagatc agcctcgacg gcctcgttct ctccttcaat ttcgagacca
     5761 aggacgttcg cctcacgaag cccctcgatc cgccgctcga tcttggctcc tcgctatcac
     5821 cgagctcacc cttagctacg gtccggatcc ggagaccaat aaggaaaagg cggttctggt
     5881 ttcgctgaag gccgttttcc gtggaaggag gagaacggcg atgcgctcga ttgggacgcc
     5941 accgaaccct gtgttgcgga tttgtggcat ggaggtgggg cggccacccc gtgcacgggg
     6001 tgggcgcgcg gggtcggaga tcggaacgaa ccagggactc tgagcccgtt tctcagcgtg
     6061 atcccgcccc gctgctcttc tgagcccgaa cggttgcatc tcggcgcggt gcggcgtctg
     6121 acggtgggca atgatgcggc cgggatcgcc cggctggtgg cctgggcaag cccgggcgcg
     6181 ctggtggtga tggaggcctc ggggggtgac gagcgcctgg cgcatcgcga cttgagcgcg
     6241 cg
//
LOCUS       DASVES010000100        20853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1165112, whole genome shotgun sequence.
ACCESSION   DASVES010000100 DASVES010000000
VERSION     DASVES010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20853
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165112"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2828)
                     /locus_tag="VF240_03550"
     CDS             complement(<1..2828)
                     /locus_tag="VF240_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7174342.1"
                     /translation="MSYNVRKTVAVALSLVLGASHAYAGLIIRGGDGLVMTGADGIQY
                     EDTNGLVMTGADESLIGVNGITVTRTSGVALRWEDGLVMTGADGVTYRAERADSVSIN
                     HLNGLVMTGADGLVMTGADGTQYQAVSVRIRQADGLVMTGADGLVMTGADGLRKAEEN
                     GLVMTGADGLVMTGADTVRLVRAAEVTATGPDGSVYSVSGDGLVMTGADGLVMTGADG
                     VAMREVQGLVMTGADGFLMASVGGATGVGLQSVDPELAALLEQSTNDEHINAAIIYHS
                     AVTESDLATLRGIGVTGGTRFRVLPVVLVTATPTQLAAVSSLPTVRALYGNRTLQWTA
                     DTSRETTGLKRARADADLTQRFSNVPVSGAGVTVAVIDTGIDATHHDLAGRVARNVKL
                     ADLQGLPSLDFSYPVNLEGLQNTDQAYGHGTHVASVVAGEGKRSGGRYAGYAPGARLV
                     GLSAGEANLFFVLGGFDYILQNRAALGIRVVNCSFSANTLFDLDDPVNVATKMLTDAG
                     VNVVFSAGNTGPGHGSLNPYAAAPWVISVGSTDARGRPSKFSSRGEFASGMFRPSVVA
                     PGEAVVGARASGVNVTGAAGVVGDTGLTNGEQKYYTTASGTSFSAPQVAGAIALMLQA
                     NPNLRPSDVRAILRRTSTPLQPFLSHEVGSGMLNTHAAVLEAAFPARYISEYRGSLDR
                     GQVAFYKDAPQEFAGAAMPGQWTEVGLTIPDGALLASVQIAWGPFQSANDLALYMDDP
                     AQTTRGSSNGINLPGLTGKRERAVLTKPQPGQWNARVAHTVGLAATLQDFSGTLEIGR
                     VDYAPLADTSAYAGTAAYDDIRQALRHFVMLPDGDLFRPDAAATRADAAAALLMGARV
                     PLYVAAQPMFTDVTDPWLRSYVESAHMAPAGAIFPNVSAGGVFNPSGQLERLTAAVAL
                     VRAAGLEAEAQASAGEPLAYTDAAQIPLP"
     gene            complement(3096..6461)
                     /locus_tag="VF240_03555"
     CDS             complement(3096..6461)
                     /locus_tag="VF240_03555"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX7174343.1"
                     /translation="MANQPHRQDQFQAITSPLPQISGEAAALAPTFFLRTKLLPPRPA
                     PTLLPRPRLVERLAQNFANPVTLVTANAGSGKTTLVADFVRAHAQQFVWYQLDHTDTD
                     PYVFLGYITYGIKLIVPGFGESTFAYLKEAARELAQQPERAVDVLLNEILDRVEQKFV
                     LVLDDYHHLGADTPVHRIVDRLLTYLPDLIHVVIISRDLPPLALARRRTQSAVSFIDR
                     EELLFTDEETRELFRKVFDLELTPEQLSEYRERTHGWITALQLVRQVAQRQALARGAG
                     TDADAAASLDLVEVLRQSERDIFDYFAEEVFADESEDVQGLLTKVSLLDRVEPETISR
                     LYPGEQSVQKLQTLVRRNVFVTLASDGGGEEFRLHPLFRGFLQRRLRADAGRAGLAAE
                     HARCAEHFLGRGQWEQAMRHLLAAEDFDRAAQTIAEHGGAWISAGALASLASFADALP
                     APALEKYPRALAHRAEVARLRDEYDTAQSIFHRAAALLHEREDAEGEAEVLHSLATIA
                     RRRGDFETAFSYLDRAVALTDERSGVRAKCGNTRGLCLVAMGEWTEAEREFRVALKSA
                     EERGDQYYARMISHNLGMPAGIRGDFSEAMRWLRRMLRDESDTSRVPQESVAHLNLAR
                     CYWHRGEFTECEQHLDRALEASQMFNLTGLRGQIFETYGNLYRERADAARASEYYDRA
                     ERAYEEAGIDWTRFPAELLEERAMLAWLTGDLSEARALLDRLLGARGAAKDEMGAHRV
                     MLARGRVLVAQGSFEEAAADLRPSLEYFRQQGLYYNEAQAALALAACDQAAGLEGDML
                     EHLRRALDLAARYDYEYWLQREVTRNTHLFSTPDAAELLPADLREQVASVSGRPEAQR
                     AERAVVAAPSAPAVDLSINMLGSVEIFRDPARPLAADAWVTRRARDILCFIASRRHRR
                     ASKDTIIDTFWPDADFEAVEKNFHPTVSHIRKGLNSNQPLKQNFLLYRDGDYQLNPEF
                     SYFMDTEEFDRLLAEGEAARRARDTETSVRAFESAIKLYRGEFVSGSYDDWVEEQRTY
                     YREQYLRLLEMLAAAAQKAEDWPRSQLLAQHILREDPFREDVHCMVMRSQAAQGNRVA
                     VREQYEMLRKLLRKELGVEPAAETQRTYKDLIK"
     gene            complement(6645..7472)
                     /locus_tag="VF240_03560"
     CDS             complement(6645..7472)
                     /locus_tag="VF240_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7174344.1"
                     /translation="MRTIDSLPVEILPEKITPPSELPRISRARLNRTLEESLDSCNST
                     IIIGRTGTGKTMLARDFSRLCARRAAWYKVDAPDAELRVFFRYLCASVAAARPGFGRK
                     TLARLGERFGEEDVPQLVEYFVYELLERDEPLLVVVDDLHLVYDAEWVVPFFRRLLPL
                     LPPEVHLVLIGRSLPPAPLWRMRSKQTLCVIDEPSLAFSPEEAEHLFESYGLPCWMAQ
                     SALEQTRGRACTLDMRARTAKVNEEAAKLSASRDDDRRERNLQLVKGFQRKSSLGTA"
     gene            complement(7847..8779)
                     /locus_tag="VF240_03565"
     CDS             complement(7847..8779)
                     /locus_tag="VF240_03565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7174345.1"
                     /translation="MAEDFIVFSGTANRPLAERVAGELGRPLGACTVEHFSDGEVFVR
                     LDESVRGREVFVVQPTSPPVNEHLVELLAFADACRRASAARVTAVVPYFGYSRSDKRQ
                     GERVAVAASMVADLLQTVGVDHVVTVDVHAAQVEGFFRVPFDNLSATPLLANSLRARL
                     PEGAVVVSPDAGRVTAASEFADCLGTEVVVLQKRRTSGTETEVTHIVGDVKDRACLII
                     DDMISTGGTIAKSVEALVGAGARPEFIVAATHGLFVGQARRKLSGGAIKEIVVTDTVA
                     VDTEGWPGLRVVSVAPLVAAAMRKSFGEGEPERK"
     gene            complement(8860..9306)
                     /locus_tag="VF240_03570"
     CDS             complement(8860..9306)
                     /locus_tag="VF240_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7174346.1"
                     /translation="MDWRSNLIENTSGVKELLAQTKRVAVLGIRSEQHSSRPAFYVPH
                     YLASAGLDVIPVPVYDLHVEEILGQKVYHNLADIPGEVDLVDVFRRSEDIPPHLDDIL
                     AKMPKAVWFQSGIRNDEAAERLARAGIKVVQDRCLMVDHRSYGPHR"
     gene            complement(9425..11137)
                     /gene="rpoD"
                     /locus_tag="VF240_03575"
     CDS             complement(9425..11137)
                     /gene="rpoD"
                     /locus_tag="VF240_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7174347.1"
                     /translation="MMEKALENNGIRPLVLGGEEYLTFEDMSGEDRQGEGISTDKIED
                     VLQKLGGDGHAGPEDLLIDDVESAASHGADAFDDSELDLSAGEEDKGSDPVRLYLRQM
                     GRVPLLTREQEVTLAKRIEAGQIRANRAIARSPIAIAELLKMGEELEDGLSGIRAVVN
                     FGDQAEFGEEEDRGEEYLRLTVEGIGRIRRLYHAGLKEAVKLYAEQKLARNKKTKKLL
                     RLRARVARARLDIARAYKALNLTEDAKERLVASITSVHKEIRTAEREIERLTEKLGRK
                     KIKPEDEREWKRRISADRRRLRQIEETTHLSIVDIKRSHQSIAKGTAQSIGAKSEMTE
                     ANLRLVVSIAKKYINRGLPFLDLIQEGNIGLMRGVEKFDWRRGYKFSTYATWWIRQAI
                     TRAIADQARTIRIPVHMIETINKIIKTSRALVQEFGREPSDDEIAKRVDMTPAKVRAI
                     LKLAQQPVSLETPIGDDGDASLGDFIEDKSKVNPSDRVVATNLREVTDEVLQTLSPRE
                     EKVIKMRFGLSTEGEEHTLEEIGENFDVTRERIRQIEAKALKKLRHPSRARLLKNFVS
                     GTQS"
     gene            complement(11306..11866)
                     /locus_tag="VF240_03580"
     CDS             complement(11306..11866)
                     /locus_tag="VF240_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7174348.1"
                     /translation="MKGFKNKILGIAAVLTLAASAGTAAPVQAQTQRASFEQVSKKVR
                     KELVTLPYFGVFDNLAYKIDGGTVTLYGQVVRPSTRKDAERSVARIDGVERVVNQIEV
                     LPLSGFDDEIRYRTFRELQRTGNLFYFMRGANPSIHIIVNRGHVTLEGFVNNEGDRRL
                     ANFAARSVPGVFSVTNNLRAERGGKY"
     gene            complement(11895..12362)
                     /locus_tag="VF240_03585"
     CDS             complement(11895..12362)
                     /locus_tag="VF240_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7174349.1"
                     /translation="MNDFAERMSGLMIDHYQRHLAELELTLPQAQALRILRRGPVTTG
                     RLAETLGVSAPALTQLTDRLARKELIERRASEGDRRCVIVALTAKGTAVVDEFRARRR
                     EAFCGALGGLSEPQREQVFSALELVVGALDTNGRGATVVLPKVSKQTGKIVRV"
     gene            12393..13001
                     /locus_tag="VF240_03590"
     CDS             12393..13001
                     /locus_tag="VF240_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174350.1"
                     /translation="MLVLASKSLIIRKIFDALSNFSVAPTPQPKIISVILAFSFKIDS
                     NLVKARGRAMLMDHGISEIYAEGRGTDGRALSVYRALRELLCSACGAPIAEGSLFTRK
                     NLPQFALRIMPRCGECAPFGMANLAEGVMPAGGGGGSSNSVEGELPKGVGAKKRSKLI
                     EGLLNAAPETETPRKPERSRDVAEKVASRLGPALSRSRRGRS"
     gene            complement(12981..14243)
                     /locus_tag="VF240_03595"
     CDS             complement(12981..14243)
                     /locus_tag="VF240_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7174351.1"
                     /translation="MEAICQERAGRPTVLEHGLQPHLDALARFSSTRELEASSLYGGL
                     RPEVERVLGGVVVEDHRRASVRAAATEGTVRATAWNIERGKQFENILRVLAEAPSMRE
                     SDVLLLTELDYGMARTENRFVARELAERLGLAFAFAPCYVALNKGSGVESGVGGENTL
                     ALHGNALLSRFPLTGAHSIALPNGKDKMRGKEKRLGNQRAVVADVLHPRGSFRAVSLH
                     LDAHSTQRHRLRQMRVVLDHLEHLRPRLPVVIGGDWNTSTYNSKRAAYSIAGYFRRVA
                     MGVGHVLRHHYPHPERWFERQLFRELERRGYGYRDLNEMGAGTLHYDVSDLATHENLR
                     EWVPAWCFAFIRWALKQNDGRCSLKLDWFAGRHIEPAPGASPKVVGGLSEIYGPVSDH
                     DPIVLDFVLSLPASNTTKPARGLRPSTP"
     gene            complement(14341..15738)
                     /locus_tag="VF240_03600"
     CDS             complement(14341..15738)
                     /locus_tag="VF240_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174352.1"
                     /translation="MGNPLGSSARVLSRAALANADKADGGFSPLAVTAGALGSPDLIA
                     HDDALYDRGVVRVITNLPGGTLEVFQGTIVPWNAQEKQITEAKFLDVLAEELARRLIG
                     IGHGDGPDPEFVAVARGMLERFIATLIPPGARMERVLRAALNLPADLNVPVHVTEFVS
                     GLLSKVTPLIENLETILRLAEELERMEGARSPDDLEQAALRLFMSAGVMYLPPIGGPK
                     KWSFREFVTKVGNRVHTLLQAHYWLAHPNDVIMFEDFIVAGPNLLLKIWDAGTWKAPY
                     DFMVALRVALMGKTRPGQDTGSTKRPDILNLTRQHLYEIKTVSQVLDGVRQVADYQRR
                     LAAVGITVHLAGMGPADWRPFPLYYVGGTTVVAVEAVRPGVIAYQRIGSRVPVTNPLR
                     FWSQESLKYQQRQAQRAQAVQQGALVAVALIGTLLLAVALAPAAVAIGGAAIAGTGAE
                     AALGLGVASGLTFAL"
     gene            complement(15716..18454)
                     /locus_tag="VF240_03605"
     CDS             complement(15716..18454)
                     /locus_tag="VF240_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174353.1"
                     /translation="MPDTPNEPMIKLEPGGVAPVLSYNVPEPTSYSGRNFIDLQLGDS
                     YFKNDDAPTAASSKSLESLVTGAAGERASAGRASLMEGTSGTAETPSLAVALKAVDAP
                     PSPKLATLDLAEVRAMQGLGKQLCIYRSLYGTLTYTYIEPPAADSAQAAGAPPVSARA
                     GSNGTEASAIDSDPGGGGGGGGGAIIAILVEITGPQSGATVNGPSGGAALHVTGVASV
                     QRGGGSISTVSVRVGSADFQLATPVAANNWATWSYDTVVTTPGELTIEARAAHSTGKT
                     ATHSITVQVALAPPADTTAPGLGITYPENGQELPGTSAGSAAVNVAGTASDVQSGVRL
                     VEVKIDDGQFSEATRGGPDWASWTKVVNLTGAGFHAITARSTDNAGNITTKTVSVKVV
                     AAPPADTTDPAVNITAPAPNAEIAGPFNGASISVTGTASDVGGGVSKVEVLVGGIATP
                     AQPRAAGDWSTWAATVMLRETGLQMIVARCTDSSGNESASAVPVNVTLIPDVDSRLKR
                     LILVESYRLSSYLGSYGAGRTIKTFSLLPGEKTKISIKTFTKTEEDAKSASSILDSFT
                     QESSDDFEESMGEEQSNKKNYDESFQYKVEATAQASWGWGSAGISGGLSGGTNSARED
                     FSKNISNATQKHVAKASAKRDVKIETSYEVKTSEGEETSVERVIENINVSRTLNFVFR
                     QMNQEFITLLHLVDARVGYFNVVHVQGQPEPRYSYREATLPELDALLAEVVVPEKQAE
                     VRADIVRQLSNIFDYKDRHHRFAEEEPLTDRDGKIVPLSQYLRVRKDYTSTYLDEATG
                     TQLTVPGIIMAASKYVLRTEGVIVEALLGQGDSLDAYSHGLQDESVEERRLANERAKV
                     EIEKERLALQIIQSKNAEAAKLFALLYPPPTQLVPYVPPAPAPASQEAPDGKPVRK"
     gene            complement(18745..19062)
                     /locus_tag="VF240_03610"
     CDS             complement(18745..19062)
                     /locus_tag="VF240_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174354.1"
                     /translation="MTTNRLPIACDLTVFGDNERKRQKSILDEFRAKVKGMEELPDGY
                     AFTLAREPYMLQLVAEMISFESRCCQFLDFTLEVKRGGTSITLRLTGGEGVKDFLREE
                     LSL"
     gene            complement(19137..19706)
                     /locus_tag="VF240_03615"
     CDS             complement(19137..19706)
                     /locus_tag="VF240_03615"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX7174355.1"
                     /translation="MRIAFIIYDGMTALDFVGAFDPLTRLKTMGFVPGVEWDVCALAR
                     EVSDGAGLSIAASKVGGALDGYDLLVVPGGYSARELAGESAFVGWLQTAADCPLKASV
                     CTGSLLLGAAGFLRGKRATTHPTAFAELEEYCREVSRERVVDEGDVVTAGGVTASIDL
                     GLHLCERIAGREVRALVQEQMDYRRVEVS"
     gene            complement(19761..20459)
                     /locus_tag="VF240_03620"
     CDS             complement(19761..20459)
                     /locus_tag="VF240_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7174356.1"
                     /translation="MSELKQTDRTELGRHPERGVFGRERVYEILDEGLVCHVGFAVGG
                     KPYVIPTGYVRHGDNLYVHGAAASRMMNVLREGVDVCVTVTLLDGLVLARSAFSHSMN
                     YRSVVVFGRARVVEDAREKLEALRAFTEHVMPGRWGEARRPNREELSETLVLAVPLEE
                     ASAKIRTGPPKDKETDYELPVWAGVVPLKLEVGTPVADPRLVAGTDVPRYVNDYRASD
                     GHAERAAGRELWSE"
     gene            complement(20628..>20853)
                     /locus_tag="VF240_03625"
     CDS             complement(20628..>20853)
                     /locus_tag="VF240_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174357.1"
                     /translation="GGLFLWVELPERVRAEELFEEALRERVAFVPGAPFFAAKARHNF
                     MRLNFSNSPPEKIEEGIRRLGNVLSKMVAG"
BASE COUNT         3319 a         6742 c         7087 g         3705 t
ORIGIN      
        1 ggcaacggaa tctgcgcggc gtcggtgtag gccaacggct cgcccgcgct cgcctgcgct
       61 tccgcttcaa gacccgccgc gcgcacgagc gcgacggccg ccgtcagacg ctctagttgc
      121 cccgaggggt tgaagacccc gccggcagag acgttgggga agatcgcacc ggccggcgcc
      181 atgtgcgcgc tctcgacgta gctgcggagc cacgggtcgg tcacgtccgt gaacatcggc
      241 tgcgccgcga cgtagagcgg cacgcgcgcg cccatgagca gggccgcggc cgcgtcggcg
      301 cgcgtggccg ccgcgtcggg gcggaagagg tcgccgtcgg gcagcatcac gaagtggcgg
      361 agcgcctgcc ggatgtcgtc gtaggcggcc gtgccggcgt aagcagatgt gtccgcgagc
      421 ggcgcgtagt caacgcggcc gatctccagc gtgccgctga agtcctgcaa ggtggcggcg
      481 aggccgacgg tgtgcgcgac gcgcgcgttc cactgtccgg gctgcggctt cgtgagcacg
      541 gcgcgctcgc gcttgccggt gaggcccggc aagttgatgc cgttcgagga ccctcgcgtg
      601 gtctgggccg ggtcgtccat gtagagggcg aggtcgttcg cgctctggaa cggcccccac
      661 gcgatttgca cggaggcgag gagcgccccg tcaggaatcg tcagccccac ttccgtccac
      721 tggcccggca tggccgcgcc ggcgaactcc tgcggcgcgt ctttgtagaa cgccacctgc
      781 ccgcggtcga ggctcccgcg atactcgctg atgtagcgcg cggggaacgc ggcttcgagc
      841 acggccgcgt gcgtgttgag catgccggag ccgacctcgt ggctgaggaa gggctggagc
      901 ggcgtcgagg tgcggcgcag gatggcgcgc acgtccgagg gccgaagatt cgggttggcc
      961 tgaagcatga gtgcgatggc gccggccacc tgcggcgcgc tgaagctcgt gcccgaagcg
     1021 gtggtgtaat acttctgttc gccattggtg aggccggtgt cgccgacgac cccggccgca
     1081 cccgtcacgt tgacgccaga ggcgcgcgcg ccgacgaccg cttcgccggg ggcgaccacg
     1141 gacgggcgga acatcccgct cgcgaactcg ccgcgcgagg agaacttcga cgggcgtccg
     1201 cgcgcgtccg tcgagccgac cgagatgacc cacggggcgg cggcgtaggg gttgagcgag
     1261 ccgtggccgg ggccggtgtt gccggccgag aagacgacgt tgacgcccgc gtccgtgagc
     1321 atcttggtgg cgacgttgac ggggtcgtcg aggtcgaaga gagtgttggc ggagaagcta
     1381 cagttgacga cgcgaatgcc gagggccgcg cggttctgga ggatgtagtc gaagccgccg
     1441 agcacgaaga agaggttggc ttcgcccgcg ctcaggccca cgaggcgcgc gcccggcgcg
     1501 tagccggcgt agcgcccgcc ggagcgtttg ccttcgccgg cgaccacgct cgccacgtgc
     1561 gtgccgtgcc cgtaggcctg atccgtgttc tgcaaccctt cgaggttgac ggggtagctg
     1621 aaatcgagcg agggcagacc ctgaaggtcg gccagcttga cgttgcgcgc cacgcggccg
     1681 gcgaggtcgt ggtgcgtcgc gtcgattccc gtgtcgatga cggccacggt cacgccagcg
     1741 ccgctgacgg ggacgtttga aaagcgttgc gtcaggtcgg cgtcagcgcg cgcgcgcttg
     1801 aggccggtcg tctcgcggct cgtgtcggcc gtccactgca aagtgcggtt gccgtaaagc
     1861 gcgcgcaccg tcggcaggct ggagaccgcg gcgagctgtg tgggcgtggc cgtgacgagg
     1921 acgacgggca ggacgcggaa gcgcgtgccg ccggtgacgc cgatgccgcg caacgtggcg
     1981 aggtctgatt cggtgacggc gctgtggtag atgatcgccg cgttgatgtg ttcgtcgttg
     2041 gttgactgtt cgaggagagc ggccagttcg ggatcaacgc tctgaaggcc cacgcccgtc
     2101 gcgccgccca cgctcgccat caggaagccg tcagcgcccg tcatcacgag gccctgcact
     2161 tcacgcatgg cgacgccgtc agcgcccgtc atcacgagac cgtccgcccc ggtcatcacg
     2221 aggccgtcgc cgctgacgct gtagacgctg ccgtcggggc cggtggccgt gacctccgcg
     2281 gccctgacca ggcgaacggt gtccgcgccc gtcatcacga ggccgtccgc gcccgtcatc
     2341 accagaccgt tctcttcggc cttgcgcagc ccgtccgcgc cggtcatcac aaggccgtcc
     2401 gcgcccgtca tcacgaggcc gtcggcctgc ctgattctga cgctcaccgc ttggtattgc
     2461 gtgccgtccg cgccggtcat cacaagaccg tcagcgccgg tcatcacgag gccattgagg
     2521 tggttgatgg agacgctgtc ggcgcgctcg gcgcggtagg tgacgccgtc ggccccggtc
     2581 atgacgagac cgtcttccca acgcagggca accccgctgg tgcgcgtgac ggtgatgccg
     2641 ttgacgccga tgagcgattc gtcggccccg gtcatcacca gcccgttggt gtcttcgtac
     2701 tggatgccgt cggccccggt catcacgagg ccgtcaccgc cgcggatgat gaggccggcg
     2761 taggcgtggc tcgcgccgag cacgagggac agcgcgacgg cgacagtttt tctgacgttg
     2821 taagacattc ttcgagacat cattccagat taagacggac gacagggaga acggttactt
     2881 gtaacgggtg actcccaagc gaggctggca tcacgtcgtc ggggaacgta tggccgaagg
     2941 ctcggatcag attgtgctcg gacgcggctt gaactgcgcc ggagattata gtctgaaccg
     3001 cgccagaagt cctaatacat tcgcgctcca tatgtgccaa aaatctaact tttctacaaa
     3061 acttgtgaca ctccgacgcc tcgcggccgg cgtcttcatt tgatcaggtc tttgtaggtg
     3121 cgctgcgtct cggcggccgg ctccacgccc agctccttgc gtagaagttt ccgcagcatc
     3181 tcgtactgct cgcggacggc cacgcggttg ccctgcgccg cctgcgagcg catcaccatg
     3241 cagtgaacgt cctcgcggaa ggggtcttcg cgcaggatgt gctgggccaa cagttgcgag
     3301 cgcggccagt cttcggcctt ctgcgcggcg gcggccagca tctccagcag acgcaggtat
     3361 tgctcgcggt agtaggtgcg ctgctcctcg acccagtcgt cgtagctgcc agagacgaac
     3421 tcgccgcgat agagcttgat ggcgctctcg aaggcgcgca ccgacgtttc cgtgtcgcgc
     3481 gcgcggcggg cagcctcgcc ctcggccaac aggcggtcga actcctccgt gtccatgaag
     3541 taggagaact ccgggttcaa ctggtagtca ccgtcgcggt agagcaggaa gttctgcttg
     3601 aggggctggt tgctgttcag tcccttgcgg atgtgggaga ccgtggggtg gaagttcttc
     3661 tcgacggcct cgaagtcggc gtcgggccag aaggtgtcga tgatggtgtc cttcgaggcg
     3721 cggcggtggc ggcgcgaggc gatgaagcac aggatgtcgc gggcgcgcct ggtgacccac
     3781 gcgtcggcgg cgagcgggcg cgccgggtcg cggaagattt ccacggagcc gagcatgttg
     3841 atactcagat cgacggccgg cgccgaaggg gcggcgacga cggcccgttc cgcgcgctgc
     3901 gcctcgggac ggcccgacac ggaggccacc tgctcgcgca agtcggcggg caggagttcg
     3961 gccgcgtccg gcgtcgagaa gaggtgcgtg tttcgggtca cctcgcgctg gagccagtac
     4021 tcgtagtcgt agcgggcggc gaggtcgagg gcgcggcgga ggtgttcgag catgtcgcct
     4081 tcgaggccgg ccgcctggtc gcaggcggcg agggcgagcg cggcctgcgc ctcgttgtag
     4141 tagaggccct gctggcggaa gtattcgagc gaagggcgga ggtcggcagc cgcctcctcg
     4201 aagctcccct gcgcgacgag cacgcgcccg cgggcgagca tcacgcggtg cgcgcccatc
     4261 tcgtctttgg ctgcgccgcg cgcgccgagc agtcggtcga gcaacgcgcg ggcctccgac
     4321 aaatcgcccg tgagccacgc cagcatcgcg cgctcttcca gaagctcggc ggggaagcgc
     4381 gtccagtcga tgccggcctc ttcgtaggcg cgctcggcgc ggtcgtagta ctcgctggcg
     4441 cgcgccgcgt cggcgcgctc gcgatagaga ttgccgtagg tctcgaaaat ctgtccgcgc
     4501 aggcccgtga ggttgaacat ctggctggct tcgagcgcgc ggtcgaggtg ctgctcacac
     4561 tccgtgaact cgccgcggtg ccagtagcag cgcgcgaggt ttaagtgggc gaccgattcc
     4621 tgcggcacgc ggctcgtgtc ggactcatcg cgcagcatcc ggcgcagcca gcgcatggcc
     4681 tcgctgaagt cgccgcggat gccggcgggc atccctaagt tgtgcgagat catccgcgcg
     4741 tagtactggt cgccgcgctc ctcggcggac ttgagcgcca cccgaaattc cctctcggcc
     4801 tccgtccact cgcccatcgc caccagacac aggccacgcg tgttgccgca cttggcgcgc
     4861 acgccggagc gttcgtccgt gagcgcgacg gcgcggtcga ggtaggagaa ggccgtctcg
     4921 aagtcgccgc ggcggcgggc gatagtggcg agcgagtgga gcacttcggc ctcgccctcg
     4981 gcgtcctcgc gctcgtgcag gagcgccgcc gcgcggtgga agatcgactg cgcggtgtcg
     5041 tactcgtcgc gcagacgcgc gacctcggcg cgatgggcca gagcgcgcgg gtatttctcc
     5101 agcgcggggg cggggagcgc gtcggcgaag gaggcgagcg aggccaaggc gccggcggaa
     5161 atccatgcgc cgccgtgctc ggcgatggtc tgtgcggcgc ggtcgaagtc ttccgccgcg
     5221 agcaggtggc gcatggcctg ctcccactgc ccgcgcccga ggaagtgttc ggcgcagcgg
     5281 gcgtgctcgg cggcgaggcc ggcacggccc gcgtcggctc tcaggcggcg ttggaggaag
     5341 ccgcggaaga gcgggtggag acgaaactcc tcgccaccgc cgtccgaggc cagcgtgacg
     5401 aagacgttgc ggcgcacgag cgtctggagt ttctgcacgc tctgctcgcc ggggtagagg
     5461 cgcgagatgg tctccggctc cacgcggtcg aggagcgaaa ccttcgtcag caacccctgc
     5521 acgtcttcgg actcgtcggc gaagacctcc tcggcgaagt agtcgaaaat gtcgcgctcg
     5581 gactggcgga gcacttcgac gaggtcgagc gaggcggcgg cgtcagcgtc cgtgcccgcg
     5641 ccgcgcgcga gcgcctgacg ctgcgccacc tgccggacga gttgcagcgc cgtgatccag
     5701 ccgtgggtgc gctcccgata ctccgagagt tgttcggggg tcagttccag atcgaagacc
     5761 ttgcggaaca gctcgcgcgt ctcctcgtcc gtgaacagga gttcttcgcg gtcgataaag
     5821 gagacggccg actgcgtgcg acggcgcgcg agcgcgagcg gcggcaaatc gcgcgagatg
     5881 atgacgacgt ggatcaggtc gggcaggtag gtcagcagcc tgtccacgat gcgatgcacg
     5941 ggtgtgtcgg ccccgaggtg gtggtagtcg tctaagacga ggacgaactt ctgctcgacg
     6001 cggtcgagta tctcgttaag gagaacgtca acggcgcgct cgggctgctg cgccagctca
     6061 cgggctgcct ccttgaggta ggcgaaggtt gactcgccga agccggggac gatgagcttg
     6121 atgccgtagg tgatgtagcc gaggaagacg taggggtcgg tgtcggtgtg gtcgagctga
     6181 taccagacga actgctgcgc gtgcgcgcgg acgaagtcgg cgaccagagt cgtcttgccc
     6241 gagccggcgt tggcggtgac gagcgtgacg gggttggcga agttctgcgc gagccgttcg
     6301 acgaggcgcg ggcgcggcag caaggtcggc gcagggcgcg gcggcagaag ttttgtgcgg
     6361 aggaagaacg tgggcgcaag cgcggccgcc tcgccggaga tttgcggcag aggggacgtg
     6421 atggcctgga actggtcttg acgatgcggc tggtttgcca tcgtccttcg gggaaacgtt
     6481 tgggagtctg cggcgcgggc tgtggcggcg gcggcgagcg gcggccactc accctgcgct
     6541 cccttcatgc tttcgacttg ggcggccatt caatttaatg caaacggccc cgtccgtgcg
     6601 aggttttttt agcgaggagt ttggcggcga gcgttacagt cccttcaggc tgtccccaaa
     6661 gaggacttgc gttggaatcc tttcaccagt tggaggttgc gctcgcgccg gtcgtcgtcg
     6721 cgcgaggcgg agagtttcgc cgcctcctcg ttgaccttcg cggtgcgggc gcgcatgtcg
     6781 agcgtgcagg cgcggccgcg cgtctgctcc agcgccgact gggccatcca gcacggcagg
     6841 ccgtagctct cgaagaggtg ctcggcctct tcggggctga aggcgagcga cggctcgtcg
     6901 atgacgcaga gcgtctgctt cgagcgcatg cgccagagcg gcgcgggcgg caggctccgg
     6961 ccgatgagca cgaggtggac ttccggcggc agcagcggca acagccggcg gaagaagggg
     7021 acgacccatt cggcgtcgta gacgagatgc agatcatcga cgacgacgag cagcggctca
     7081 tcgcgctcca gcagctcata gacgaaatac tcgacgagct gcggcacgtc ctcctcgccg
     7141 aagcgctcgc cgagacgcgc cagcgtcttg cgcccgaagc ccggccgcgc ggcggccacg
     7201 ctcgcgcaga ggtagcggaa gaagacgcgc agctcggcgt cgggcgcgtc aaccttgtac
     7261 caggccgcgc gccgcgcgca caggcgcgag aagtcgcggg ccagcatggt cttccccgtc
     7321 cccgtccgcc cgatgatgat ggtcgagttg caggagtcga ggctctcttc gagcgtccga
     7381 ttcagacgcg cccgggagat gcgcggcaac tccgacgggg gagtgatttt ttccggcaga
     7441 atctccaccg ggagtgagtc aatggttctc atggatgagg tgtgaacctg tcttgaaatt
     7501 tgtaacatca actgctttgt tcactttctg cgccgcgagg tttcgtgaca gcccggcggg
     7561 catgaactcc cccgccttag tccatcatca gagtaagacg ggccacttga ggaattcttg
     7621 aggcggcgaa aatattttcg ggcgtgctgg ggaaaggcga ctatctgatg acactgcgag
     7681 gattatggcg atgttcgggc gggcgcgacg gccgatgacg gaaggagggg gaacacggat
     7741 gacacggata agatggatgg ggcggattca atccgtgtga atccgcccca tccgtcttat
     7801 ccttgttccc cgtctccgag cggtcgcgga ataacatctg tgcggcctac ttcctttccg
     7861 gctccccctc gccgaaagac ttcctcatgg ccgccgcgac gagcggcgcg accgaaacga
     7921 ccctcagccc cggccagcct tccgtatcga ccgccaccgt gtccgtcacg acaatctcct
     7981 tgatggcccc gcccgagagc ttgcggcggg cttggcctac gaacaatccg tgcgtggccg
     8041 cgacgataaa ctcgggccgc gcgcccgcac ccacgagcgc ctccacactc ttcgcaatcg
     8101 tcccgcccgt cgagatcatg tcgtcgataa tcaggcacgc gcggtctttc acgtcgccga
     8161 cgatgtgcgt cacttccgtc tccgtcccac tcgtgcgccg cttctggagc acgacgacct
     8221 ccgtgcccag acagtcggcg aactccgacg ccgccgtcac gcgccccgcg tccggcgaga
     8281 cgacgaccgc gccctcgggc agacgagcgc gcagggagtt ggcgagcaac ggcgtcgccg
     8341 acaggttgtc gaagggcacg cggaagaagc cctcgacctg cgcggcgtgt acgtctacgg
     8401 tgacgacgtg atcgacgccg accgtctgca agaggtcggc gaccatgctc gccgccaccg
     8461 cgacgcgctc gccctgtcgc ttgtcggagc gcgagtagcc gaagtatggg acgacggccg
     8521 tgacgcgcgc ggccgacgcg cggcggcagg cgtcggcgaa ggccagcagc tcgaccagat
     8581 gctcgttaac tggcggcgag gtcggctgca cgacgaagac ctcacggccc cgtaccgact
     8641 cgtcgagcct gacgaagacc tcgccgtccg agaagtgttc gaccgtacac gcgccgagcg
     8701 gacgccccaa ctcaccggcc acccgctcgg ccagcggcct gttagccgtc cccgaaaaga
     8761 cgatgaaatc ttcagccatc cgacttatcc acctcaagca aatgtaaaga ggcgaaacgc
     8821 ttcctcactt ttgccttttg cctttttact tttgccttcc taacggtggg ggccgtaact
     8881 ccgatgatcg accatcagac agcgatcctg cacgaccttg atgccggcgc gcgccagccg
     8941 ctcggcggcc tcgtcgttgc gaattcctga ctggaaccag acggctttcg gcatcttggc
     9001 gaggatgtcg tcgagatgtg gggggatgtc ctcggaacgt cggaacacgt ccacgaggtc
     9061 aacctcgccg ggaatgtcgg cgaggttgtg atagaccttc tgaccgagaa tctcctcgac
     9121 gtgcaggtcg tacacgggca ccgggattac gtcgaggccc gccgaggcga gataatgcgg
     9181 gacgtagaag gccggccgcg acgagtgctg ctcactcctg atgccgagca cggccacgcg
     9241 cttcgtctgc gcgagcagtt ctttgacgcc gcttgtgttt tcgatgagat tgctccgcca
     9301 gtccatgcca gaaacccccg ctgtttgtat ttgtcccaaa atagcagagg cgggagggcc
     9361 gaatcaaagt cggccctccc gcctgaaggt gcgaaaggag tacggcgcgt ccccgcgcgc
     9421 gtctttaaga ctgcgtgccc gagacgaagt tcttgagcag tcgggcgcgc gacgggtggc
     9481 gcagcttctt gagcgccttg gcctcgatct ggcggatgcg ctcgcgtgtc acgtcgaagt
     9541 tctcgccgat ctcttcgagc gtgtgctcct cgccctcggt gctgaggccg aagcgcatct
     9601 tgatgacctt ctcctcgcgg ggcgagagcg tctgcaagac ctcgtcggtg acttcgcgca
     9661 ggttcgtcgc cacgacacgg tccgaagggt tgaccttcga cttgtcttcg atgaagtcgc
     9721 cgaggctggc gtcgccgtca tcgccgatgg gcgtctccag agagacgggc tgctgggcga
     9781 gcttgaggat ggcgcgcacc ttcgccggcg tcatgtccac gcgcttggcg atctcgtcgt
     9841 ccgaaggctc gcggccaaac tcctgcacga gcgcccgcga ggtcttgatg atcttgttga
     9901 tcgtctcgat catgtggacg gggatgcgga tggtgcgcgc ctggtcggcg atggcgcggg
     9961 tgatggcctg ccgaatccac cacgtcgcgt aggtcgagaa cttgtagccg cgccgccagt
    10021 cgaacttctc cacgccgcgc atcaggccga tgttgccctc ctgaataaga tcgaggaacg
    10081 gcagaccgcg gttgatgtac ttcttggcga tggagacgac gaggcgcagg ttggcctccg
    10141 tcatctcgct cttggcgccg atgctctggg ccgtgccctt cgcaatcgac tggtgcgaac
    10201 gtttgatgtc cacgatggag aggtgcgtcg tctcctcgat ctggcgcagg cggcggcggt
    10261 cggcgctgat gcgccgcttc cactcgcgct cgtcctcagg cttgatcttc ttccggccca
    10321 gcttctccgt caggcgctcg atctcgcgct cggccgtgcg aatctccttg tggacgctcg
    10381 tgatggaggc gaccagacgc tccttggcgt cctcggtcag attcagagcc ttgtacgcgc
    10441 gcgcgatatc gaggcgcgcg cgggccactc tggcccgcag ccgcaggagc ttcttcgtct
    10501 tcttgttgcg cgcaagcttc tgctcggcgt agagcttcac ggcctccttg agacccgcgt
    10561 ggtagagccg gcggatgcgc ccgatgccct cgacggtgag tcgcagatac tcctcgccgc
    10621 gatcctcctc ctcgccgaac tcggcctgat cgccgaagtt gacgacggcg cggattccgc
    10681 tcaggccgtc ctcaagctcc tcgcccatct tcaacagctc ggcgatggcg atgggcgaac
    10741 gggcgatggc gcggttggcg cgaatctgcc cggcctcgat gcgcttggcg agcgtcacct
    10801 cctgctcgcg ggtgaggagc gggacgcgtc ccatctgccg caggtagaga cggacggggt
    10861 cgctgccctt gtcctcttcg ccggccgaga ggtccaactc ggagtcgtcg aaggcgtcgg
    10921 cgccgtgcga ggcggccgac tccacgtcgt cgatgagcaa gtcttccggc ccggcgtggc
    10981 cgtcgccgcc gagcttttgc aacacgtcct cgatcttgtc ggtcgagatg ccctcgccct
    11041 ggcggtcttc accgctcatg tcctcgaagg tgagatactc ctcgccgccc aaaacgagcg
    11101 gtcggatgcc gttattctca agtgcctttt ccatcatctt tgccacgaag cccctcgcgc
    11161 cccctctaac tgatgaatgc gtagccgttt aaacacttgc gccgaaaaat acgaataaac
    11221 caatacgaga aaaccgccgg gcgggtttgc tttcgctccg ccgcccgacg atcatctcgg
    11281 aaagttcaaa aagctctccc gcgcctcaat acttgccccc gcgctccgcg cgcaggttgt
    11341 tcgtcaccga gaacacgccg gggacgctgc gcgcggcgaa gttcgccaga cgcctgtcgc
    11401 cctcgttgtt gacgaagcct tccaacgtca cgtggccgcg attgacgatg atgtgaatcg
    11461 acgggttcgc gccacgcatg aagtaaaaaa ggttccccgt ccgctgtagc tcccggaacg
    11521 tccggtagcg aatctcgtcg tcgaagcccg atagcggcaa cacctctatc tggttgacga
    11581 cgcgctcgac gccgtcgatg cgcgcgacgc tgcgctcggc gtccttgcgc gtgctcgggc
    11641 gcacgacctg cccgtagagt gtcaccgttc cgccgtcgat tttgtacgcg aggttgtcga
    11701 aaacgccgaa gtacggaagc gtgaccaatt ccttgcgaac tttcttggaa acctgctcga
    11761 agctggcccg ctgcgtctgc gcctgcacgg gcgcggccgt accggccgac gcggccagcg
    11821 taagcacggc cgcgattccc agaatcttgt tcttgaagcc tttcataata cctttaactc
    11881 ccccctgtat gttgttacac cctaacgatt tttcccgttt gtttagatac tttgggcagc
    11941 accacggttg cacccctgcc attcgtatcg agcgcgccga cgaccaattc caaagcactg
    12001 aaaacctgct ccctttgcgg ctcgctcaag ccccccagcg ccccgcagaa agcctcccgg
    12061 cggcgcgcgc ggaactcgtc caccacggcc gtccccttcg ccgtcagcgc cacgatgacg
    12121 caacgcctgt caccctcgga cgctcgtcgc tcaatcaact ctttccgggc cagacggtcg
    12181 gtcaactgcg tcagtgccgg ggcggacaca cccagtgttt ccgccagacg gcccgtagtc
    12241 acggggccgc ggcgcaggat tctgagcgcc tgcgcctgcg gcagggtcaa ctcaagctcg
    12301 gccagatggc gctgataatg gtcgatcatc aggccggaca ttctttcggc gaaatcgttc
    12361 aatatctcct ccaccttgcc tgacgccgcc acgtgctcgt cttagcctcc aagagcttaa
    12421 ttatacgaaa aattttcgat gcattaagta atttctcggt tgctccaacg ccccaaccaa
    12481 aaataatttc ggtcattcta gcattttcct ttaaaatcga ctcaaatttg gtgaaagctc
    12541 ggggccgggc gatgttgatg gaccacggca tcagcgaaat atacgcggag ggtcggggga
    12601 cggatgggcg ggcgctgtcc gtttatcgcg cgctgcggga gcttctgtgc tcggcgtgtg
    12661 gggcgccgat tgccgaaggg agcctgttca cgcgcaaaaa cttgccgcaa ttcgccttgc
    12721 gaataatgcc gcggtgcggc gagtgcgcgc ccttcgggat ggcgaacctc gctgaggggg
    12781 tgatgccggc cggcggcggc ggtgggtcgt cgaacagcgt cgagggcgag ttgccaaaag
    12841 gcgtcggggc caaaaaaagg tcgaagctca tcgaaggttt gctgaacgcc gcgccggaga
    12901 ccgaaacacc gcggaagccg gagcgttccc gcgacgttgc cgaaaaggtg gcgagccgac
    12961 tcgggccagc cctctctcgc tcacggcgtg gacggtctta acccgcgggc gggttttgta
    13021 gtgttcgacg ccggcaaaga caggacgaag tcgaggacga tggggtcgtg gtcggagacg
    13081 gggccgtaaa tctcgctcag cccgccgacg accttcggcg acgcacccgg agctggctcg
    13141 atatgacggc cggcgaacca gtcgagcttc agagagcagc gtccgtcgtt ttgcttgagc
    13201 gcccagcgga taaacgcgaa gcaccacgca ggcacccact cgcgcaggtt ctcgtgggtg
    13261 gcgaggtcgg acacgtcata gtggagtgtg ccggcgccca tctcgttgag gtcgcgatag
    13321 ccgtagccgc gccgctcaag ctcgcggaag agttggcgct cgaaccaacg ctcggggtgc
    13381 ggatagtggt gacgcaggac gtggccgacg cccatcgcca cgcggcggaa gtagccggcg
    13441 atggagtacg cggcgcgctt cgagttgtag gtcgaggtgt tccagtcgcc gccgatgacg
    13501 acaggcaggc gcgggcgcaa gtgttcgagg tggtctaaga cgacgcgcat ctgccgcagg
    13561 cggtgtcgct gcgtggagtg cgcgtcgagg tggaggctga cggcgcggaa gcttccccga
    13621 gggtgcagca cgtcggcgac gacggcgcgt tgattcccca gacgcttctc cttgccgcgc
    13681 atcttgtcct tgccgttcgg cagcgcgatg gagtgcgcgc ccgtcagggg gaaacgcgac
    13741 aggagcgcgt tgccgtgcag cgccagagtg ttctcgccgc cgacgccgct ctcgaccccg
    13801 ctccccttgt tgagcgcgac gtagcacggc gcaaacgcga acgcgaggcc cagcctctcg
    13861 gccaactcgc gcgcgacgaa acggttttcg gtgcgcgcca tcccgtagtc aagttccgtc
    13921 agcagcagca cgtcgctctc gcgcatcgac ggcgcttcgg cgagcacgcg caggatgttt
    13981 tcaaattgct tcccgcgctc gatgttccac gccgtcgcgc ggacagtgcc ctctgtcgcc
    14041 gccgcgcgca cggacgcacg gcgatgatct tcgacgacca cgccgcccag cacgcgctcg
    14101 acctccggcc tcaacccccc gtacagggac gacgcctcaa gctcgcgcgt cgaggagaag
    14161 cgcgcgagcg cgtcgaggtg cggctggagt ccgtgttcta aaacggtggg gcgcccggcc
    14221 cgctcttgac agatggcttc caccaaaacg gcttgatgtt acaggcgtgg gcgcgcgcgt
    14281 ttcaaccctc ccgctgcgtt aaccagctcc cgcccgtgag actggaatcg cgcgcagggc
    14341 ctaaagagcg aaggtgaggc ccgaggcgac gcccagcccg agggctgcct ccgcacccgt
    14401 cccggcgatg gccgccccgc ctatggccac cgcggccggg gcgagcgcga cggcgagcag
    14461 cagcgtgccg atgagcgcga cggcgacgag cgcgccttgc tgtacggcct gcgccctctg
    14521 cgcctgccgc tgttggtact tcaggctctc ctgcgaccag aagcgcagag ggttggtgac
    14581 gggcaccctt gagccgatgc gctggtaggc gatgacgccc ggccggactg cctcgacggc
    14641 gacgaccgtc gtcccgccga cgtagtagag cgggaacggc cgccagtccg ccggccccat
    14701 gccggcgagg tgaaccgtta tgcccaccgc cgcgagccgc cgctggtagt cggccacctg
    14761 cctgacgccg tcgagcacct gactgacggt cttaatctcg tagaggtgct gccgcgtcag
    14821 gttgaggatg tcgggccgct tggtcgagcc cgtgtcctgc cccggccggg tcttgcccat
    14881 cagcgccaca cgcaacgcca ccatgaagtc gtagggcgcc ttccacgtgc cggcatccca
    14941 aatcttcaag aggaggttcg gcccggcgac gatgaagtcc tcgaacatga tgacatcgtt
    15001 cgggtgtgcc agccagtagt gggcctgtaa cagtgtgtgg acgcggttgc cgaccttcgt
    15061 cacgaactcc cggaaggacc atttcttggg gccgccgatg ggcgggaggt acatgacgcc
    15121 ggcactcatg aacaggcgca gcgccgcctg ctccaggtcg tccgggctcc tcgccccctc
    15181 catccgctct aactcctcgg cgaggcgcag gatggtttcc aggttctcaa tgagcggcgt
    15241 gaccttggac agcagcccgc tgacgaactc ggtgacgtgg acggggacgt tgagatcggc
    15301 cggcaggttg agcgccgcgc gcagcacgcg ctccatgcgc gcgccgggcg ggatgagagt
    15361 ggcgatgaac cgttcgagca tgccgcgcgc gaccgcgacg aactccgggt caggcccgtc
    15421 gccatggccg atgccgatga gccggcgcgc caactcctcg gccagcacgt cgaggaactt
    15481 cgcctccgtg atctgcttct cctgcgcgtt ccacggcacg atagtcccct ggaagacttc
    15541 gagcgtcccg ccggggaggt ttgtgatgac gcgcacgacg ccgcggtcgt agagggcgtc
    15601 gtcgtgggcg atgaggtctg gcgagccgag cgcgccggcc gtgacggcga ggggcgagaa
    15661 ccccccgtcc gccttgtcgg cgttggcgag cgccgcccgg ctcagaactc tcgcactact
    15721 tcctaacggg tttcccatcc ggggcctcct gtgacgcggg cgccggggcc ggcgggacgt
    15781 agggtacgag ttgggtcggc ggcgggtaga ggagggcgaa gagcttggcg gcctcggcgt
    15841 tcttggattg gatgatttgc agcgcgaggc gttccttctc gatctcgacc ttcgccctct
    15901 cgttggcgag cctccgctcc tcaaccgact cgtcctgcaa tccgtgcgag taggcgtcga
    15961 gggagtcgcc ctgcccgagc agcgcctcga cgatgacgcc ctcggtgcgc aggacgtact
    16021 tgctggccgc cataatgatg cccggcacgg tgagctgcgt ccccgtcgcc tcgtcgagat
    16081 aggtcgaggt gtagtccttc ctgacgcgca ggtactgcga gagcgggacg atcttgccgt
    16141 cgcgatccgt aagcggctcc tcctcggcga agcggtggtg gcgatccttg tagtcgaaga
    16201 tattcgagag ctggcggacg atgtcggcgc gcacctcggc ctgcttctcg gggacgacga
    16261 cttcggcgag cagcgcgtcc agctccggca gcgtcgcctc gcggtagctg taccgcggct
    16321 cgggctgccc ctgcacgtgg acgacgttga agtagccgac gcgcgcgtcc acgaggtgca
    16381 gcagggtgat gaactcctgg ttcatctgcc ggaagacgaa gttgagcgtg cggctgacgt
    16441 tgatgttctc gatgacgcgc tcgaccgagg tctcttcgcc ctcggaggtc ttgacctcgt
    16501 agctggtctc gatcttcacg tcgcgcttgg ccgacgcctt cgcgacgtgc ttctgcgtgg
    16561 cgttcgagat gttcttggag aagtcttcgc gggccgagtt ggtgccgccc gagaggccgc
    16621 cgctgatgcc ggcgctgccc cacccccagc tcgcctgcgc cgtcgcctcg accttgtact
    16681 ggaagctctc gtcgtagttc ttcttgttgg actgctcctc gcccatcgac tcctcgaagt
    16741 cgtccgagct ttcctgcgtg aaggagtcga ggatgctcga cgcgctcttg gcgtcttcct
    16801 cggtcttggt gaaggtcttg atgctgatct tggtcttctc gccgggcagc agcgagaagg
    16861 tcttgatggt gcgcccggcg ccgtagctgc cgaggtacga ggaaaggcgg taactctcga
    16921 cgaggatcag gcgcttgagg cgcgaatcga catcggggat cagcgtgacg ttgacgggga
    16981 cggcgctggc gctctcgttg cccgaggagt ccgtgcagcg ggcgacgatc atctgtaacc
    17041 ccgtctccct gagcatcacc gtcgccgccc acgtagacca gtcgccggcg gccctcggct
    17101 gcgccggcgt ggcgatgccg ccgacgagca cctcgacctt gctcacgccg ccgcccacgt
    17161 ccgaggccgt gccggtgacg ctgatgctcg ccccgttgaa cggcccggcg atctcggcgt
    17221 tcggcgccgg cgccgtgata ttcaccgccg ggtcggtcgt gtcggctggc ggcgccgcga
    17281 ctactttgac gctcacggtt ttcgtcgtga tgttgccggc gttgtcggtc gagcgcgccg
    17341 tgatggcgtg aaacccggcg cccgtgagat tgaccacctt cgtccaactc gcccagtcgg
    17401 ggccgccgcg cgtcgcctcg gagaactgcc cgtcgtcaat cttgacctcg accagtctga
    17461 ctccgctctg cacgtcggag gccgtcccgg cgacgttgac ggcggccgag ccggccgacg
    17521 tgccgggcaa ctcctgtccg ttctcggggt aggtgatgcc cagtccgggg gccgtcgtgt
    17581 cggcgggcgg ggcgagcgca acctgcaccg tgatgctgtg tgtagcggtc ttgccggtac
    17641 tgtgcgcggc acgggcctcg atggtgagtt cgcccggcgt ggtgacgacg gtgtcatacg
    17701 accacgtcgc ccagttgttg gcggcgaccg gcgtcgcaag ttggaagtct gccgacccga
    17761 cccggacact gaccgtggag atcgagccgc ctccgcgctg cacgctcgcc acgcctgtca
    17821 cgtgaagggc ggccccgccg cttggcccgt tgaccgtggc ccctgactgc gggccggtga
    17881 tttcgacgag gatggcgatg atggcgccgc cgccgccccc gcccccgcct ccggggtcgc
    17941 tatcgatggc gctggcctct gtcccgttcg agccggctct ggcagagacg ggcggtgcgc
    18001 cggcggcttg ggcagagtcg gccgcgggcg gttcgatgta ggtgtaggtc agcgtcccgt
    18061 agagcgagcg gtagatgcag agctgtttgc cgagtccctg catggcgcgt acttcggcga
    18121 ggtcgagggt ggcgagtttc gggctggggg gggcatcgac ggctttcagg gcgacggcca
    18181 gcgacggcgt ctcggcagtc cccgacgtgc cctccatcag ggaggcccgc cccgccgagg
    18241 cgcgttcgcc cgcggccccg gtcacgaggg attcgaggga cttcgatgac gcggccgtcg
    18301 gggcgtcgtc gttcttgaag tacgagtcgc cgagttgaag gtcgataaaa ttccggccgc
    18361 tgtaggaagt cggctcaggc acgttgtagc tgagcacggg cgcaaccccg ccgggttcga
    18421 gcttgatcat cggttcgttt ggtgtgtcgg gcatggtctc gcaactcctg tcgggtgaga
    18481 agtgtgggtt gccggagggc gggctttggg agcgccgcgc cgccgagttt cggagtatgg
    18541 ctttctgacg aacgggggca aacagggaga gacgttactg agcgtgaggg atcatgctac
    18601 tcgcacgacg gcgaagcgcg tgcggctgaa cagcgtggtc aaagatgcta ctgccgtgag
    18661 cagggcggat tatagtgaga attcttgttt tgtaaagatt ttgagcgact atagaaagag
    18721 tctcgaccag ggaagacgtg ccgcctacag cgacagctct tcgcgcagga aatccttcac
    18781 gccctcgccg ccggtcagtc ggagcgtgat tgacgttccg ccgcgcttca cttcgagggt
    18841 gaagtcgagg aactggcagc agcggctctc gaaggagatc atctcggcga cgagctggag
    18901 catgtaaggc tcgcgggcga gggtgaaggc gtagccgtcg ggcagttctt ccatgccctt
    18961 caccttcgcg cgaaactcgt cgagtatcga cttctgccgc ttcctttcgt tgtcgccgaa
    19021 caccgtcagg tcgcaggcga tgggaagtct gttcgtcgtc atgtctttac accaccccgc
    19081 tcgggcgctc tcgccggttt ctttttttgt tgacgctgga tgcgcgcgcg taagtttcac
    19141 gacacttcaa cgcgacggta gtccatctgc tcctgaacca gagcgcgcac ttcgcggccg
    19201 gcgatgcgct cgcacaggtg caggccgagg tcgatggagg ccgtgacgcc gccggccgtg
    19261 accacgtcgc cctcgtccac gacacgctcg cgcgaaactt cgcggcagta ttcttccagc
    19321 tccgcgaaag ccgttgggtg cgtcgttgcc cgctttccac gcaggaagcc ggcggcgcca
    19381 agcaggagcg accccgtaca aacggacgcc ttcagcgggc aatcggcggc cgtttgcagc
    19441 cacccgacga aagcgctttc gccggccaac tcccgcgcgc tgtagccgcc cggcacgacg
    19501 agcagatcgt aaccgtcgag cgccccaccg accttcgacg ccgcgatgct tagccccgcc
    19561 ccgtcgctca cctcgcgcgc cagagcgcac acgtcccact ccacacccgg cacgaacccc
    19621 atcgtcttca gacgcgtcaa cggatcgaac gccccgacga agtcgagcgc cgtcatgccg
    19681 tcatagatga tgaaggcgat tcgcataaga aagcggtcat ctatcagtta tcagctaaat
    19741 gctaatagct gataacttca tcactcactc cacagctcac gcccggccgc gcgctcggcg
    19801 tggccgtcgg acgcgcggta gtcgtttacg tatcgcggca cgtccgttcc ggccacgagt
    19861 cgcgggtcgg cgaccggcgt gcccacttcg agttttaagg ggacgacgcc cgcccagacc
    19921 ggcagctcgt agtcggtctc cttgtccttc ggcgggccgg tgcggatttt ggccgacgcc
    19981 tcttcgagcg gcacggcgag cacgagcgtc tccgacagct cctcgcggtt cggccggcgc
    20041 gcctcgcccc agcggcccgg catgacgtgc tcggtgaaag cgcgcagggc ttcgagtttt
    20101 tcgcgcgcgt cctcaaccac acgggcgcgg ccgaagacga cgaccgagcg gtagttcatc
    20161 gagtggctga aggccgagcg cgcgagcacg aggccgtcga ggagcgtcac cgtcacgcac
    20221 acgtcaacgc cttcgcgcaa tacgttcatc atccggctgg ccgcggcgcc gtggacgtag
    20281 aggttgtcgc cgtggcggac gtagcccgtc gggatgacgt agggtttgcc cccgacggcg
    20341 aagccgacgt ggcacaccag tccctcgtcg agaatctcgt agacgcgctc tcgcccgaac
    20401 acgccccgct ccgggtgcct gccgagttcg gttctatcgg tctgcttcag ctcactcatc
    20461 ggcgatgctc ctgatccttg cccacttgaa tttgaacggc ggggttattc accgcagagg
    20521 cgcagaaaac gcaaaggggg cgcggagagt tgaaccctct gcgtgaactc cgcgccctct
    20581 gcgcctccgc ggtgaatctt ctttaaactc actcaagtta atgttactca tccagccacc
    20641 atcttcgaca acacgttgcc gagacggcgg atgccctctt cgatcttttc cggcgggctg
    20701 ttcgagaagt tgaggcgcat gaagttgtgc cgcgctttgg cggcgaagaa gggcgcgccg
    20761 gggacgaagg ccacgcgctc gcgtagcgcc tcctcgaaca actcctcggc gcggacgcgc
    20821 tccggcaact cgacccacag aaacagcccg ccc
//
LOCUS       DASVES010000101         7976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_623619, whole genome shotgun sequence.
ACCESSION   DASVES010000101 DASVES010000000
VERSION     DASVES010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623619"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..657
                     /locus_tag="VF240_03630"
     CDS             178..657
                     /locus_tag="VF240_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7174358.1"
                     /translation="MSDKVKPIPEGYHTVTPYVCVSDAARAIEFYKEALGATEIMRME
                     APGGRIGHAEIKIGDSHVMLADEFPEINFRSPQTLGGPTAQFMIYVEDVDARVEQAVA
                     AGAKLVRPVKDQFYGDRSGGVEDPFGHFWYISTRIENLTFEEVKKRSDAEMAQHAGA"
     gene            857..1141
                     /locus_tag="VF240_03635"
     CDS             857..1141
                     /locus_tag="VF240_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX7174359.1"
                     /translation="MKITGFIWLEEVVRKLSRKHSVEQDEVAEVFLNAPRFRFVEKGH
                     RSGENVYAALGRTDAGRYLICFFVHKQEGRALILSARDMSRAERRRYDKK"
     gene            1128..1391
                     /locus_tag="VF240_03640"
     CDS             1128..1391
                     /locus_tag="VF240_03640"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEX7174360.1"
                     /translation="MTRSKSSISKAKSYKEIGEYWDSHDLGENWDETEPAEFEVSIQG
                     RANLVALDEAVKDKLTEIARSKKVPVEVLVNEWLKEKIASHHK"
     gene            complement(1436..1645)
                     /locus_tag="VF240_03645"
     CDS             complement(1436..1645)
                     /locus_tag="VF240_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174361.1"
                     /translation="MKSRSLRRHHEERIKRRVRKYYGGVHDGDLRRIGQMAHARKLCS
                     CWMCGSPRRYFGERTLRERRLDGES"
     gene            1704..2432
                     /locus_tag="VF240_03650"
     CDS             1704..2432
                     /locus_tag="VF240_03650"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX7174362.1"
                     /translation="MKHPAGTSIIDDVDEKVLPPSFKGRQYEVRALGLRDFAGLRRDD
                     DALDPFELAYFARLMVINFEQIEGLSEETKEHLLGAGTDAWSGGTCSRPLPNGWRLVI
                     LNPKHGRSRNNATLMEEVCHVFLGHKANRLSIVARNKNGQTVARDYNDDDEEAAYSVG
                     AAALAPYAGLRKLMRQGKTSIEIARHFNVSRELVEFRLKISRLWAEYRERHPEETAAR
                     AARRNGFAARAGAQADNAPVPEKD"
     gene            2476..3606
                     /locus_tag="VF240_03655"
     CDS             2476..3606
                     /locus_tag="VF240_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX7174363.1"
                     /translation="MDRASETPQERLRRLRDDIKRTIDYRSFYLRYCPEARQAGARLL
                     SLCPIPSHGHSGQGSPSLSIDTQHGLFNCFSRGEGGDHITFYELMHGVNFGRAVRELA
                     RELGLTGRRPRQPPLSQRAAPDAAGPETFEPLDAERTTEICEAFLRVCRGEDQIEGVR
                     YLSRRGIGPETVRRAGVVYFPRRAYRRVMRRMLDAFPPEELQRSGLFNRRAHLTFYRH
                     RLLFPFLVEGRARYLQARTTAPGVEPRWHNMRGSVPALYNADALAELPTDSVVYLVEG
                     FTDTLTLAARGFAAVGLVGAGGLREDWLAPLARFRVVAALDPDAAGRRAAARYAEMFA
                     RRRMRLASVALPTDINDFFRQHPSASLELSLLAEAALEDSEQ"
     gene            3697..4995
                     /locus_tag="VF240_03660"
     CDS             3697..4995
                     /locus_tag="VF240_03660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX7174364.1"
                     /translation="MADDLRQAARPMAGLSVVDKLRLLLDITKKISRSLSLQEVLMMV
                     MDTLDSLLPYDAAGIYLIRRDQHLSEGGTATLVFHAEAMRGYDVEEMMELRLKAGQGL
                     IGHVAQTGEPIICPDVRLDPRYVNARRATRSEMVAPIISNDEVIGVFDLESDHLDAYT
                     EDDKEVLMLLTSQVAIIIEKAMLHEQLVEKKRLEAQLEVARQVQLSLLPARDPTLEGF
                     DISAYNFSTEEVSGDYYDFVNIYEDQLGLVIADVSGKGVPAALLMAFLRASLRAAIHI
                     GYAPHVAMSKVNYLLWESIERNQFVTAFYGVLDSTNKTLAYTNAGHNPPLMLDAGGEA
                     RFIERGGLPLGMFRDARYYEYYLAVEAGQTLVLYTDGVTEAADAKGEEYGRERLERAV
                     REGRDLAARALIDFIYADVLAWTGGRGATDDVTFVVIKAL"
     gene            5124..7682
                     /locus_tag="VF240_03665"
     CDS             5124..7682
                     /locus_tag="VF240_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7174365.1"
                     /translation="MNIELSDNTALSHYRISHKLGAGGMGEVYLAEDTLLRRKVALKV
                     LPESLAQDKDRLRRFEQEAFAASALNHPNILTIYEFGVEGETHFLASEYIDGETLRAR
                     LQHATFTLDEALDVAVQTAQALAAAHEAKIIHRDIKPENIMFRRDGYVKVLDFGLAKL
                     CEREPSSTSAGSEYPTKVLIRTEAGAVFGTAPYMSPEQARGIPVDARTDIWSLGVVIY
                     EMLTGHRPFSGQTHTDIIVSVVSTEPPPISSYGREIPAELAWIVSKALSKDVAGRYQT
                     AAELRVDLEKIRKQIEFEESVNRSAGRHLPKDATEKEKLPSGVAPAHPTSNTADKRPS
                     DGLDGAAAPRDFWSSLRPAGYSRLVKTQRVTYSILALALLAVVSAGVYFVFNTSGDNR
                     RIDSIAVLPFENLSGNPELTFVSDGLSEALIDRLSQLPQLKVISRYSSFAFRGANHNL
                     RDVAEKLDVRAIVTGNVAQVGDDLVVRFDVADVFENRQLTGGQFRRKPGDLLSIQSEI
                     AQTATKQLQVKLSDAQSTRLVENGTENSEAYRSYLSGLVELNGPQDVRGRALEYFERA
                     VTLDPDFAAAHAEIAWVYWSRANGSSDPQALVPKAKAATERALTIDPEIAKAHVLKAM
                     VSEYEFDVQGAEREYRRALELSPNLGFARGYYAFFLSVMGRQDEALAELEQQGTRDPL
                     NQRLTLLHKGMILTQARKFDEALQAYGEAQALEPEREIPYFSLGYAYAGKGLYNEAAA
                     YYRKSISLLGGEEKYSQPLVYLAATYARMPEKRREARAMLTRIEAMSDYSSPALLAAV
                     HSALDDNDKAMELLERAYIKHDPLLRFIGTGYEYDGLRADRRFIDLTRRVGVGR"
BASE COUNT         1598 a         2475 c         2420 g         1483 t
ORIGIN      
        1 gttcgagaca gaagttttgc gcggggcgct cgactcaagg cagggttgag cgcctttttt
       61 cttgtaacgc gtttctactt gtaccaagag actcctcgct ttgggccgga gtgacaaccg
      121 ttaatccggc ggccatctga aactcccgat cagatcaaac ctgtatagga gaaaatcatg
      181 tccgacaaag tgaaacccat ccccgagggc taccacaccg tgacgcctta cgtgtgcgtg
      241 agcgacgcgg cccgcgccat cgagttctac aaggaggcgc tcggcgcgac cgagatcatg
      301 cgtatggagg cgcccggcgg gaggatcggg cacgccgaga taaaaatcgg cgactcgcac
      361 gtcatgctcg ccgacgagtt tcccgaaatc aacttccgca gcccgcagac gttgggcggc
      421 ccgactgctc aattcatgat ctacgtcgag gacgttgacg cgcgcgtcga gcaggcggtc
      481 gccgccggcg cgaagctggt gcggcccgtc aaggatcagt tctacggcga ccgcagcggc
      541 ggggtggaag accccttcgg ccacttctgg tacatctcca cgcgcatcga gaacctgacg
      601 tttgaagagg tcaagaagcg cagcgacgcc gagatggcgc aacacgcggg agcatgaagc
      661 gcgactgacc gaaacatcgt gcatcagtct gcggtagcgg gtgggtcttc tcccgccgcc
      721 acactcgccc gctatcgcag gcaatactga ctcgacgctt cgccggagcg gttgcatcca
      781 atcgcaaaag tgttggctat atctaaagtt gaagagtctc gctatttcac aggtcataac
      841 gatttaaggt caacgcttga aaatcaccgg cttcatttgg ctcgaagagg tcgtgcggaa
      901 gctttcgcgg aagcattctg tcgagcagga tgaggtcgca gaggttttcc tcaacgcccc
      961 gcgtttccgt tttgttgaaa aggggcaccg ttcaggcgaa aatgtttacg cggcgctggg
     1021 gcggacggac gccggccgct atctgatatg cttttttgtt cataagcagg aggggcgtgc
     1081 gttaatcttg tccgcccgcg acatgagcag ggccgaacgg agaagatatg acaagaagta
     1141 aaagctccat ttcaaaggct aaatcttata aagagatcgg cgagtattgg gattcgcacg
     1201 acctgggcga gaactgggac gagaccgagc cggccgagtt cgaggtgagt attcaagggc
     1261 gggcgaatct cgtcgcactt gacgaggcag tgaaggacaa gctaaccgag attgccaggt
     1321 cgaagaaggt tccggtcgag gtgctggtca acgagtggct taaagaaaag atagccagcc
     1381 accacaagtg aagccgcgcc gttttcaatt ccgccgcgtt gagccagcat ccgcgtcacg
     1441 actccccatc taagcggcgc tcccgaagcg tgcgctcccc gaagtaacgg cgtggacttc
     1501 cgcacatcca acacgagcag agtttgcgtg cgtgggccat ctggccgatg cgacgcaggt
     1561 cgccatcatg tacgccgccg taatacttcc tgacgcgacg cttgatgcgt tcctcgtgat
     1621 ggcgtctgag tgagcgtgat ttcatggcgt cgtgaatgac atcagtttag cgcaagactt
     1681 ttcttccagc atagcctttt gaagtgaaac accccgccgg gacgagtata atcgacgacg
     1741 tggacgagaa ggttctaccg ccgagtttta aggggagaca gtacgaggtg cgcgcgttgg
     1801 ggttgcgcga cttcgcgggc ctgcgccggg acgacgacgc gctcgacccg ttcgagctgg
     1861 cgtacttcgc gcggttgatg gtgattaact tcgagcagat cgaggggctg tcggaggaga
     1921 cgaaggagca tcttctgggg gcgggcacgg atgcgtggtc gggcgggacg tgttcgcggc
     1981 cgctgccgaa cggctggcgg ctcgtcatcc tgaatccgaa gcacggccgg tcgcgcaaca
     2041 acgcgacgct gatggaggag gtctgtcacg tcttcctcgg tcacaaggcg aaccgcctgt
     2101 ccatcgtcgc ccgcaacaag aatggccaga cggtcgcgcg cgactacaat gacgatgacg
     2161 aggaggcggc ctactcggtc ggggcggcgg cgctggcgcc ttacgcgggt ctgcgtaagc
     2221 tgatgcgaca gggcaagacc tcaatcgaga tcgcgcgcca cttcaacgtg agccgggagc
     2281 tggtcgagtt tcgtctgaag atttcgcgtc tgtgggcgga gtaccgcgag cggcaccccg
     2341 aggagacggc cgcgcgcgcc gcccgccgca acggcttcgc cgccagagcc ggcgcgcagg
     2401 ccgacaacgc gcccgttccc gagaaggact gacgccagcg cgcccaccga agacctgttc
     2461 ggccaccgac ctcctatgga tagagcgagc gagacgccgc aagagcgcct gcgccgtctg
     2521 cgcgacgaca tcaagcgcac gattgactat cgctcgttct acctgcgcta ctgccccgag
     2581 gcccgtcagg cgggcgcgcg tctcctgagt ctctgcccca tcccctcaca cggacacagc
     2641 gggcagggca gccccagcct ctccatcgac acacagcacg gactcttcaa ttgcttctcg
     2701 cgcggcgagg gcggcgacca catcaccttt tacgagctga tgcacggcgt caacttcgga
     2761 cgcgccgtgc gcgagctggc gcgcgaactc ggcctcaccg gccggcgtcc acgccagccc
     2821 ccgctctcgc agcgcgccgc cccggacgcc gccgggccgg agaccttcga gccgctcgac
     2881 gcggagcgca ccaccgaaat ctgcgaagcc ttcctccgcg tctgccgcgg cgaagaccag
     2941 attgaaggcg tcaggtatct ctcgcgacgc ggcatcggcc ccgagactgt caggcgcgcg
     3001 ggggtcgtct acttcccgcg ccgcgcctac cgccgcgtga tgcgccggat gctcgacgcc
     3061 ttcccacccg aagagcttca gcgcagcggc ctcttcaacc ggcgcgcgca cctcaccttc
     3121 taccgacacc gcctgctctt cccgtttctc gtcgagggcc gcgcccgcta tctacaggcg
     3181 cgcaccaccg cgcccggcgt cgagccgcgc tggcacaaca tgcgcggcag cgtccccgcg
     3241 ctctacaacg cggacgcgct cgcggagctg cccaccgact cggtcgtcta tctcgtcgaa
     3301 ggtttcacgg acactctgac actcgcggcg cgcggcttcg cggccgtcgg actcgtcggg
     3361 gccggcggac tcagggagga ctggctcgcg ccgctggcgc gcttccgcgt cgtcgccgcg
     3421 ctcgaccccg acgccgccgg ccgccgcgcc gccgcgcgct acgccgagat gttcgcgcgc
     3481 cgacggatgc gcctcgcctc cgtcgcactg cccaccgaca tcaacgactt ctttcgccaa
     3541 cacccctccg cctcgctgga gctttcgctg ctggcggaag cggcgctgga agacagtgag
     3601 cagtgagcag tgagcagtaa gcagtcagca cgctcaacca gcatttactg ctcactgctc
     3661 actgcttact gctcactctc ctgatataat ttccgtatgg ccgacgacct cagacaggcg
     3721 gcgcgcccga tggccgggct aagcgtggtg gacaagctgc gcctcctgct cgacatcacg
     3781 aagaagatca gccgctccct gagccttcag gaagtgctca tgatggtcat ggacacgctc
     3841 gactcgctgc tcccctacga cgcggccggc atctatctca tccgccgcga ccagcacttg
     3901 agcgagggcg gaaccgccac gctcgtcttc cacgccgagg ccatgcgcgg ctacgacgtg
     3961 gaggagatga tggagctgcg tctgaaggcc gggcagggac tcatcggaca cgtcgcgcag
     4021 acgggcgagc cgatcatctg ccccgacgtg cgcctcgacc cgcgctacgt caacgcccgc
     4081 cgcgccaccc gctccgagat ggtcgcgccc atcatctcga acgacgaggt catcggcgtc
     4141 ttcgatctgg agagcgacca cctcgacgcc tacacggagg acgacaaaga ggtgttgatg
     4201 ctgctcacct cgcaggtggc catcatcatc gagaaggcca tgctgcacga gcagctcgtg
     4261 gagaagaagc gtctggaggc gcagctcgaa gtcgcccggc aggtgcagct ctccctgctc
     4321 ccggcccgcg accccaccct cgaaggcttc gacatcagcg cctacaactt ctcgaccgaa
     4381 gaggtctccg gcgactacta cgacttcgtc aacatctacg aagaccagct cggcttggtc
     4441 atcgccgacg tgtcggggaa gggtgtcccc gccgcgctcc tgatggcctt cctgcgcgcg
     4501 tcgcttcgcg ccgccatcca catcggctac gcgccccacg tcgccatgtc gaaggtcaac
     4561 tatctgctgt gggagtccat cgagcgcaac cagttcgtca cggccttcta cggcgtcctc
     4621 gacagcacga acaagacgct cgcttacacg aacgccgggc acaacccgcc gctcatgctc
     4681 gacgccggtg gcgaggcccg cttcatcgag cgcggcggcc ttccgctcgg catgttccgc
     4741 gacgcgcgct actacgagta ttatctggcg gtcgaagcgg ggcagacgct cgtgctctat
     4801 acggacggcg tgaccgaggc ggccgacgcg aagggcgagg agtacggccg cgagcgtctg
     4861 gagcgcgccg tgcgcgaagg ccgcgacctc gccgcgcgcg ccctcatcga cttcatctat
     4921 gccgacgtgc tcgcctggac gggcggccgc ggcgccaccg acgacgtgac cttcgtcgtc
     4981 atcaaagctc tctgacaact ccgcgcgccc gtcgggcgtc cggtcgaagt gcgctgcgtt
     5041 tatctactgc tgttgatggc ggcctacctg ctcaccgcca tcggccgcaa ggtcatcacc
     5101 gccggcgagt tcgacaatct gcaatgaata tagagctatc agacaacaca gccttatctc
     5161 attaccgtat cagccacaaa ctcggcgcgg gggggatggg cgaggtgtat ctggccgaag
     5221 acacgctgtt gcgccgtaag gttgcgctta aggttctgcc cgaatctctc gcacaggaca
     5281 aagaccgtct gcgacgcttc gagcaggagg cctttgccgc ttcggccttg aatcatccaa
     5341 acatcctgac gatttacgaa ttcggtgtgg aaggcgaaac gcattttctc gcctcggaat
     5401 atattgacgg tgagactttg cgcgcccgct tgcaacacgc gaccttcacg ctcgatgaag
     5461 ctctggacgt tgccgtgcag acggcgcagg ccctcgccgc cgcacacgaa gcgaaaatca
     5521 ttcaccgcga catcaagccc gaaaacatca tgttcaggcg tgacgggtac gtcaaggtac
     5581 tcgactttgg gctggcaaag ctttgtgaac gtgaaccctc gtcaacgagc gcggggtctg
     5641 agtatccgac caaggtcctg attcgcaccg aggctggcgc cgtatttggc acggccccct
     5701 atatgtcgcc ggaacaggct cgcggcattc ccgtcgatgc caggacggat atctggagcc
     5761 tgggcgtggt catctatgaa atgcttacgg gccacaggcc gttttccggt caaacccaca
     5821 ccgacatcat cgtctctgta gtttcgacag aaccgccccc catttcgtca tacgggcggg
     5881 agattccggc ggaacttgcg tggatagttt caaaggcgct ctcaaaagac gtggcgggac
     5941 gatatcaaac cgcggcagaa ctgcgggttg atctggagaa gatcagaaag cagatcgaat
     6001 ttgaagaaag tgtaaaccgt tcggcaggcc gccatctgcc caaagacgcg acggagaaag
     6061 aaaagcttcc gtccggcgtt gcaccagccc acccgacatc aaatacggct gacaaaagac
     6121 cgagcgacgg actcgatgga gccgccgccc cgcgtgattt ctggtcgtcg ctccgcccgg
     6181 caggttattc acggctggtt aaaacccaga gggtcactta ctcgatcctt gctctggctc
     6241 tgttggcagt ggtttccgcc ggcgtctatt ttgttttcaa tacatctggt gacaatcggc
     6301 ggatagattc gattgccgtc cttccgtttg agaatttaag cggtaaccca gagctgacct
     6361 ttgtctcaga cggcctgagc gaagccctga tagaccggct ctcgcagttg cctcagttga
     6421 aagttatctc tcgttattcg agttttgcgt ttcgcggagc gaatcacaac ctgcgcgacg
     6481 ttgccgaaaa actggatgtt cgggcgattg ttacgggcaa tgtggctcaa gtcggcgatg
     6541 atctggtcgt caggtttgac gttgcggatg tttttgagaa caggcagctc acgggggggc
     6601 aattccggcg caagcccggc gaccttctga gcattcagag tgagatagcc cagacagcca
     6661 ccaagcaact gcaagtaaaa ctgtctgatg cgcagtcaac acgtctcgtt gagaacggga
     6721 cggaaaactc cgaagcttac cgctcttacc tgagcggcct ggttgaatta aatgggccgc
     6781 aggacgtgcg gggccgggcg ctcgaatatt tcgagcgggc agtaacgctc gatccagatt
     6841 tcgcggcggc ccatgccgag atcgcctggg tctactggtc acgggcgaac ggcagcagcg
     6901 acccgcaagc actggtgccg aaagcgaagg cggcgacgga gcgagccctc acaatcgacc
     6961 ctgagatcgc gaaagcccat gttctcaagg ccatggtgag cgaatatgag ttcgacgtgc
     7021 agggggccga gagggagtac cggcgggcgc ttgagttgag tcctaatctc ggcttcgctc
     7081 gcggttatta tgcgttcttt ctttcggtga tggggcgaca agacgaagcc ctcgccgaac
     7141 tcgaacagca ggggacccgc gatccgctca accagcgcct gaccctgctc cataaaggta
     7201 tgatcctgac gcaggcgaga aaatttgatg aagcactaca ggcgtacggg gaagctcagg
     7261 ccctggaacc ggagagagag atcccctact tttctttggg atatgcctat gccggaaagg
     7321 gtctctataa cgaggccgcg gcgtattaca ggaaatcgat cagtctgctc ggcggcgaag
     7381 agaaatatag tcaacccctc gtctatctgg ccgcgactta tgcccggatg cccgagaagc
     7441 gccgcgaggc gagagctatg ctgacgagga tagaggccat gagtgactac tcttctccgg
     7501 cccttttagc ggccgttcat tcggcgctcg atgacaatga caaggcgatg gagttactcg
     7561 aacgggcata catcaagcac gatccgttgt tgcggtttat aggaaccggt tacgagtatg
     7621 acgggcttcg cgctgatcgg cgcttcatcg atctgacgag gcgagtcggt gtcggacgtt
     7681 agggagggca gtgtcggacg gcaaccttcc caagagcatg acctgcttgc aagacacttg
     7741 ttgggaaagc atcgagaaac gggaagcccg ttcgatatca tctacggcgg atgatgaaga
     7801 gtttagctgt cagaattctg cgtctccggc gccggctcct caggcttttt cgtaaaaccc
     7861 gaaatgtccc atcggctctc gatattcttg aagagccgca agctgtcaaa gaacgcgttc
     7921 gagccgccgc ccccaagttt ggagccttcc tgacctgcaa gctttgaacc ttcctg
//
LOCUS       DASVES010000102         9753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_235275, whole genome shotgun sequence.
ACCESSION   DASVES010000102 DASVES010000000
VERSION     DASVES010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235275"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VF240_03670"
     CDS             complement(<1..639)
                     /locus_tag="VF240_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX7174366.1"
                     /translation="MKTSLLAFLLSGLACLSAGCSGGGGPRNDSASNGGVVAPSPQTA
                     TAPARDNLPKIVALGDSLTAGYGLADVRQSYPALLQKKLDADGYQYEVINAGVSGGTS
                     AGGLRNLDWALAGDVRFVIVELGANDHLRGQPVAETRKNLSEIIRRARGRGAEVILAG
                     MLTTANAGDRYLREIADMYTELAREHKVTLIPFFLEGVAGIDALNLEDRVHPN"
     gene            638..1372
                     /locus_tag="VF240_03675"
     CDS             638..1372
                     /locus_tag="VF240_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7174367.1"
                     /translation="MIELYKVSKTVRSGETNLTILHELDLTIPTGQFIAVVGPSGSGK
                     STLLGLVAGLDSPTSGEIRIDGREITGIGEDALAELRGVKIGIVFQSFHLIPSLTAFE
                     NVLVPMEIAGARGAAEKARRLLEDVDLTARAHHYPSQLSGGEQQRVAIARAFSNNPAI
                     LLADEPTGNLDSRNGRHVFDLMIEMNRRRGATLVLVTHDHELAALADRRISLRDGRVV
                     SDSGDAGEAETARDEFEAEGLEAARK"
     gene            1369..3894
                     /locus_tag="VF240_03680"
     CDS             1369..3894
                     /locus_tag="VF240_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7174368.1"
                     /translation="MNFIFQMAWRELRAGWRRLLFFFLCIGIGVGAVVALRTVIRNVN
                     RAMMGEAQQLLTADALVDSTRPWSAETLAKIERVARPPLVEARTEAIEASTMARPADD
                     TREGAQMVELKGIEPPFPLYGEFKLQGGERFDHAMLEGGGAIVAPLLLERLGLSVGDA
                     MKIGETVYQIRAVLEQEPGAGGGFRLGPRVFVARGSMEDAGLTGFGSRARRRIFFKTA
                     DAGVTELVARLRAELAGDLVNVRSYRESQENMSEDFERGENFLALTGLVVLVLGGIGV
                     AGVTRVFIEQKKKSIAILKCVGATGKRITAAYLAQTLALGVAGSLLGIALAKAALVYV
                     AGRFAASLPANMSYELSAGAVWQGLGLGVLISVLFSALPLLRVRRIKPDILLRDRDDE
                     GPWWGRFDLPRLATGALLAASLFALAVWQAGSLRVGAWFLAGLAATSGLLYLAAWALV
                     FVVRRARRVGSFALRQAISGLHRPGNQTRVVIMAVGLGAFLVMSVLQLQSNLLDELDF
                     SRRGQLANMYLIDIQKDQAEGVARIIEETTGKPADLVPTVRMRIAAIDGRDINLEEVR
                     EGRGMLGREYVVTYRPRLEQNETIVAGEFWGEEPSAEPEVSIEEGMRGVGGLDVGTRI
                     TFDVLGRKLTARVSSVRKVDWRNSRTGFLVLFRPGTLENAPQSMVGAINGPVDAQARA
                     RFQRAILDRYPNVSVIDVFDVVRSIGRILGNITLAVTFIGGFVLLCGTLILVGSIAMT
                     KYQRVYEAAVLKTLGAKRRTLLTILLAEYGLLGMVAGAVGALAAIALSYAVAKYMLKI
                     PWTFEPSVVLGGVAATAALVVIVGALSSFDVLVRKPLATLRGQ"
     gene            complement(4214..5563)
                     /locus_tag="VF240_03685"
     CDS             complement(4214..5563)
                     /locus_tag="VF240_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEX7174369.1"
                     /translation="MRSINKQSSHITLSSSLLFIFCLLTAGVVAGAQTGGGRRVDAEK
                     FPGADLGAKINAADKSLGAGAGEIVVSSGGTVRTQVVISPGHTLRFASGTYASEVGGS
                     TYLLKDNTSMRCGRGAVLLESTAPDPTHGGVGPLGSSLFTIVQDHAGSTLNGNISRNI
                     TVEGCTFRGARQDFNSAYQTISMGNCQNCRIEGNRLENTRTIGMQFGGAAAGGNYALN
                     SVMARNEFIGVASQNAAITNCVGCRVENNRFINPGQVNGPGVTVIDIEPNVGDRVDDI
                     LISNNHIDATLTPLDASGPKTTNGIAANAGNQTVMFRNVRIVGNTVIGASHSAPWSRI
                     QYAGILVRTAPGVTVSGNTLRRLNRGIVVDHDSTDFTIRENTLISCGTGGTASFSIER
                     SSGGRVISNELRDEPGDAMGLGPRALIIYEEPPSDNNLFQGNRANVQKTGRRSRVVQ"
     gene            complement(5720..7381)
                     /locus_tag="VF240_03690"
     CDS             complement(5720..7381)
                     /locus_tag="VF240_03690"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7174370.1"
                     /translation="MGRQKGARGGSPLSRRDFLKTGAVGGALALVAPLAARSSANAEN
                     VASHRAADSFELEETTVAELQAGMRSGKWSAKELTEKYLERIERVDRRGAALNSVIEL
                     NPEAISIAEALDRERKAGRTRGPLHGVPVLIKDNIDTADRMLTTAGSLALVESRPKQD
                     AFIVERLRAAGCVLLGKTNLSEWANFRSTHSTSGWSARGGQTRNPYVLDRNPCGSSSG
                     SGAAVAASLAAVGVGTETDGSIVCPASANSLVGIKPTLGLVSRSGIIPIAHSQDTAGP
                     MARTVADAAALLGALAAQDPRDSITREGAGHAHADYTRFLDAGALRGARLGVARKHFG
                     FNDRVDKLMSESIDALKRLGAVIIDPADIPTTGKFDDSELEVLLYEFKADLNKYLAAR
                     GPEQKTRTLKDLIAFNERNRAREMPYFGQELFIRAEAKGPLTSRAYLTALRKNQLLTR
                     SQGIDAVMRRRRLDAMVAPTGGPPWPTDLVSGDHFTGGYSSASAVAGYPHVTVPAGYV
                     FGLPVGLSFFGGAWSEPALIKFAYAFEQATRARRPPKFTPAAVLG"
     gene            complement(7436..7915)
                     /locus_tag="VF240_03695"
     CDS             complement(7436..7915)
                     /locus_tag="VF240_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174371.1"
                     /translation="MPVAHVNAPHYERAAEVETLVRDFVSCTLPWSDWTHAAHLTVAL
                     WHLLHFDWAEAVERVRRRIKRYNAANGVETTRERGYHETLTLFWMHYVRFFLEANYNE
                     GCSLASLANTLIERAERNAPLTHYSRELLFSWEARLAWVEPDLKALPAVDSLSLTGA"
     gene            complement(8633..>9753)
                     /locus_tag="VF240_03700"
     CDS             complement(8633..>9753)
                     /locus_tag="VF240_03700"
                     /inference="COORDINATES: protein motif:HMM:NF017541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HEX7174372.1"
                     /translation="RDDAAVFAGSVGQQPSLADIVRMVHPRPRTPEREALFGYLVGRE
                     HDARRLPLLVQQFEAFKAGDTLDVPDVPFQMLTALPLGTSDWTAIARRAPWQMTRMNL
                     NTFARHGVFEQPGVAELVAQRLADPEKVRKARVFPYQLLAAYVSTDERVPQLVRDALQ
                     DALEVATTNVPRIDGKVYVLPDVSGSMHSAVTGQRRGSTSAVRCIDVAALVAATLLRK
                     NPSAEVIPFESDVVKVQLNPRDSVMTNAQKLAALPAGGTNCSAPLRRLNERKAEGDLV
                     VYVSDQESWVDAPQYGRFGGSHTETMREWAQFKARNPRARMVCIDLQPYGTTQAVERE
                     DILNVGGFSDQVFDVLAGFAQGRHDAGHWVEVIEGVAL"
BASE COUNT         1569 a         3074 c         3418 g         1692 t
ORIGIN      
        1 gttcgggtgg acgcggtctt cgaggttgag cgcgtcgatg ccggcgacgc cttcgaggaa
       61 gaaggggatt aaagtgactt tgtgctcgcg cgcgagttca gtgtacatgt cggcaatctc
      121 gcgcaggtat cggtcgccgg cgttggccgt cgtgagcatc ccggcgagaa tcacctccgc
      181 gccgcggcct cgcgcgcggc ggatgatctc gctcaggttc tttctggtct cggcgacggg
      241 ttggccgcgc aggtgatcgt tggccccgag ttcgacgatg acgaagcgca cgtcgccggc
      301 gagcgcccag tcgaggttgc gcaggccccc ggccgacgtg ccgcccgaaa cccccgcgtt
      361 aattacctcg tactgatatc cgtccgcatc taacttcttc tggagcagcg ccgggtagct
      421 ctgtcgcacg tccgcgaggc cgtaaccggc cgtcaggctg tcgccgagcg cgacgatttt
      481 aggtaaattg tccctggcgg gcgcagtcgc agtttggggc gacggggcga cgacgccgcc
      541 gtttgacgcc gaatcattac gagggccgcc gccgccggag caaccggccg acagacaggc
      601 gaggcccgat agaaggaaag caagcaggga agtcttcatg atcgaattat acaaggtatc
      661 caaaaccgtc cgctcgggcg aaacgaactt aaccatcctt cacgagcttg acttgaccat
      721 cccgacgggg cagttcatcg ccgtcgtcgg cccctcgggc agcggcaagt ccacactgct
      781 cggcctcgtc gccggcctcg actccccgac ctccggcgag attcgcatcg acggccggga
      841 gattaccggc atcggcgagg acgcgctggc cgagttgcgc ggcgtcaaga tcggcatcgt
      901 cttccagtcg ttccacctca tcccgtcgct cacggcgttt gagaacgtgc tcgtgccgat
      961 ggagatcgcc ggcgcgcgcg gcgccgccga gaaggcccgc cgcctgctcg aagacgttga
     1021 cctgacggcg cgcgcgcacc actacccttc gcaactctcg ggcggcgagc agcagcgcgt
     1081 ggccatcgcc cgcgccttct ccaacaaccc cgccatcctc ctcgccgacg agccgaccgg
     1141 caacctcgac tcgcgcaacg gccgccacgt cttcgacctg atgatcgaga tgaaccggcg
     1201 tcgcggcgca actctcgtcc tcgtcacgca cgaccatgag ctggccgcgc tcgccgaccg
     1261 tcgcatctcc ctacgcgacg ggcgcgtggt gtctgacagc ggcgacgcgg gcgaggcgga
     1321 gacggcccgc gatgagtttg aggccgaggg gttggaggcg gcgcgcaagt gaacttcatc
     1381 ttccagatgg cttggcgcga gctgcgcgcg gggtggcggc ggctgttgtt tttctttctg
     1441 tgcatcggca tcggcgtcgg cgcggtggtg gccctgcgca cggtcatccg caacgtgaac
     1501 cgcgcgatga tgggcgaggc gcagcagctt ttgacggccg acgcgctggt cgattccacg
     1561 cggccgtggt cggcggagac gctcgcgaag atcgagcgcg tggcccggcc gccgctcgtc
     1621 gaagcgcgca cggaggccat cgaggcttcg acgatggcgc gacccgccga cgacacgcgc
     1681 gagggcgcgc agatggtcga gctgaagggc atcgagccgc ccttcccgct ctacggtgag
     1741 ttcaaattgc agggcggcga aaggttcgac cacgcgatgc tcgaaggcgg cggggccatc
     1801 gtcgcgccac tgctgctcga acgcctcggc ctgagcgtgg gcgacgcgat gaagatcggc
     1861 gagaccgtct accagattcg cgcggtgctc gaacaggagc cgggcgcggg cggcggcttt
     1921 cgtctggggc cgcgcgtctt cgtcgcgcgc gggagcatgg aagacgcggg actgacgggc
     1981 ttcgggagcc gcgcgcgccg gcgcatcttc tttaagacgg cggacgcggg cgtcactgaa
     2041 ctcgtcgcgc gcctgcgcgc ggagctggcc ggcgacctcg tcaacgtccg ctcttacagg
     2101 gagtcgcagg agaacatgag cgaggacttc gagcgcggcg agaacttcct cgcgctgacg
     2161 ggcctcgtcg tgctcgtcct cggaggcatc ggcgtcgccg gcgtgacgcg cgtcttcatc
     2221 gaacagaaga agaaatcaat cgccatcctc aagtgcgtcg gcgcgaccgg gaagagaatc
     2281 acggccgcgt atttggcgca gacgctcgcg ctcggtgttg ccggcagcct cctcggcatc
     2341 gcgctggcga aggccgcgct tgtttacgtg gccgggcgct tcgccgcgag cctgccggcg
     2401 aacatgagct acgagctgag cgcgggcgcc gtctggcagg ggctggggtt gggcgtgctc
     2461 atctccgtac tcttctccgc gctcccgctg ctgcgcgtcc gccgcatcaa gcccgacatc
     2521 ctcctgcgcg accgcgacga cgaaggccca tggtggggcc gcttcgacct gccgcgcctc
     2581 gcgacgggcg cgctgctcgc cgcgtcgctc ttcgcgctgg ccgtgtggca ggcggggtcg
     2641 ctgcgcgtcg gcgcgtggtt cctcgcgggg ctggcggcca cttcggggct gctctacctc
     2701 gcggcgtggg cgctcgtctt cgtcgtccgg cgcgcgcgcc gcgtcggctc cttcgcattg
     2761 cgtcaggcca tcagcggcct gcaccgcccc ggtaaccaga cgcgcgtcgt catcatggcc
     2821 gtgggcttgg gcgcgtttct cgtgatgtcg gtgctgcaac tacagtcgaa cctcctcgac
     2881 gagctggact tctcgcgccg cggacaactc gccaacatgt acctcatcga catccagaaa
     2941 gatcaggccg agggcgtcgc acggataatc gaggagacga ccggaaagcc ggccgatctt
     3001 gtcccgacgg tgcggatgcg tatcgccgcc atcgacggcc gcgacatcaa tctcgaagag
     3061 gtgcgtgagg ggcgcggcat gctcgggcgc gagtacgtcg tcacctaccg gccgcgtctt
     3121 gaacagaacg agacgattgt ggcgggcgag ttctgggggg aggagccgtc ggccgagccg
     3181 gaggtgtcca tcgaggaggg gatgcgcggg gtgggcggtc tcgacgtggg cacgcgcatc
     3241 accttcgacg tgctcgggcg caagctcacg gcgcgcgtgt cgagcgtgcg caaggttgac
     3301 tggcgcaact cgcgcacggg tttcctcgtg ctcttccggc cgggcacttt ggagaacgcg
     3361 ccgcagagca tggtcggcgc catcaacggc cccgtggacg cgcaggcgcg cgcgcgattc
     3421 cagcgcgcca tcctcgaccg ctacccgaac gtctccgtca tcgacgtgtt cgacgtagtc
     3481 cgcagcatcg gccgcatact cggtaacatc acgctggccg tcaccttcat cggcggcttc
     3541 gtgctgctgt gcgggacgct catcctcgtc ggctccatcg ccatgacgaa ataccagagg
     3601 gtttacgagg cggccgtctt aaagacgctc ggggcgaagc gccggacgct tctgaccatc
     3661 ctgctcgccg agtacgggct gctcgggatg gtggccggcg ccgtgggcgc gctcgccgcc
     3721 atcgccctct cctacgcggt ggcgaagtac atgctgaaga taccgtggac gttcgagccg
     3781 tcggtcgtcc tcggcggcgt ggccgcgacg gccgccctcg tcgtcatcgt cggcgcgctg
     3841 tcgagcttcg acgtgctggt gcggaagcct ttggcgacgc tgcgcggaca gtgaggacaa
     3901 ggcaaaagtt aaaaggcaaa aggcaaaagt gctaacgccg aaccgcaggc gtcaacgagc
     3961 cgctgtaagc ggcggaagaa tgtagcccac ggcgtgagcc gtgggtcagc cgttaagaaa
     4021 aacagggaag ccccggaggg gcgaaagaat ctcaggccga tgcatcgcgc acgtcttgat
     4081 tcttccgccc ctccggggct tcgttaattt tatccctcgc gtccccacgg ctcacgccgt
     4141 gggctacatt ctatcgccgc cccggcggct ggttgacgcc tgcgattccg ctcttccact
     4201 tttgcctttt gccttactgc acgacgcgcg agcggcggcc cgtcttctga acgttggcgc
     4261 ggttgccctg gaacaggttg ttgtcggagg gcggctcctc gtagatgatg agggcgcgtg
     4321 ggccgaggcc catcgcgtcg cccggctcgt cgcgcagctc gttggagatc acgcgaccgc
     4381 ccgacgaacg ctctatggag aatgaggcgg tgccgcccgt gccgcaggag atgagcgtgt
     4441 tctcgcggat ggtgaagtcg gtgctgtcgt ggtcaacgac gatcccgcga ttgaggcggc
     4501 gcagggtgtt gccggagacc gtgacgccgg gcgcggtgcg gacgaggatg ccggcatatt
     4561 gaatgcggct ccagggcgcg ctgtggctcg cgccgatgac ggtgttgccg acgatgcgga
     4621 cgttgcggaa catgacggtc tggttcccgg cgttggcggc gatgccgttg gtcgtcttcg
     4681 ggccgctggc gtcgaggggc gtgagcgtgg cgtcgatgtg gttattgctg atgaggatgt
     4741 cgtccacgcg gtcgccgacg ttgggctcga tgtcgatgac cgtgacgccg gggccgttca
     4801 cctgtccggg gttgatgaag cggttgttct cgacgcggca gccgacgcag ttggtgatgg
     4861 cggcgttctg cgaggcgacg ccgatgaatt cgttgcgggc catcacggag ttgagcgcgt
     4921 agttgccgcc ggcggccgcg cccccgaact gcatgccgat ggtgcgcgtg ttctcaagtc
     4981 ggttgccctc gatgcgacag ttctgacagt tgcccatcga gatggtctgg taggccgagt
     5041 tgaagtcttg gcgcgcgccc ctgaaggtgc agccctcgac ggtgatgttg cgggagatgt
     5101 tgccgttcaa ggtggagccg gcgtgatcct gcacgatggt gaagagtgaa cttccaagcg
     5161 gcccgacgcc gccgtgggtc gggtcaggcg cggtgctttc aaggaggact gcgccgcgcc
     5221 cgcagcgcat ggaagtgttg tccttgagca gatacgtcga gccgccgacc tccgaggcgt
     5281 atgtgcccga ggcgaagcgc agcgtgtggc cgggcgagat gacgacctgc gtgcgaaccg
     5341 tcccgcccga ggagacgacg atctcgcccg cacctgctcc gagtgatttg tcggcggcgt
     5401 tgattttggc tccgaggtct gcgccgggga acttctcggc gtcaacccgc cggcccccgc
     5461 ccgtctgcgc tccggcgacc acacccgctg tgagcagaca gaaaatgaac agcagcgacg
     5521 acgacagggt tatgtgtgac gactgtttat tgatgctacg catagtttga agacccttgt
     5581 tcgcccgcga cgcgggagtg tgaaaataaa ttcttccgag aaggttaaag ggggagtgac
     5641 gtgcgccgtt gtttggttgc gcgggcgcgg gggagcgttg gacgaatagt gcgccgacga
     5701 ggcggccggg ggccgtcatt cagccgagga cggccgcggg cgtaaacttc ggtggccggc
     5761 gggcgcgggt cgcctgctcg aaggcgtagg cgaacttgat aagtgccggc tcgctccagg
     5821 cgccgccgaa gaaggagagg ccgacgggga ggccgaagac gtagccggcg gggacggtga
     5881 cgtgcgggta gccggcaacg gccgaggcgg aggagtagcc gccggtgaag tggtcgccgc
     5941 tgacgaggtc ggtcggccag ggcggcccgc ccgtgggcgc gaccatcgcg tcgaggcggc
     6001 gccggcgcat gacggcgtcg atgccctgcg agcgcgtgag gagttgattc ttgcgaagcg
     6061 ccgtgagata ggcccgactc gtgagcggcc ccttcgcctc ggcgcggatg aaaagctcct
     6121 gaccgaagta gggcatctcg cgcgcgcggt tgcgctcgtt gaaggcgatg aggtctttga
     6181 gcgtgcgcgt cttctgttcg gggccgcgcg cggccaagta cttgttgagg tcggccttaa
     6241 actcgtagag caaaacctca agctcggagt cgtcgaactt ccccgtagtg gggatgtcgg
     6301 ccgggtcgat gatgacggcg ccgaggcgct tgagcgcgtc gattgattcc gacatcaact
     6361 tgtccacgcg gtcgttgaag ccgaagtgct tgcgggcgac gccgaggcgc gcgccgcgca
     6421 aggccccggc gtcgagaaag cgcgtgtagt cggcgtgggc gtggcctgcg ccctctcgtg
     6481 tgatggagtc gcgcgggtct tgcgcggcga gtgcgccgag caaggcggcg gcgtccgcaa
     6541 cggtgcgggc catcgggccg gcggtgtcct gactatgcgc gatggggatg atgccggaac
     6601 ggctgacgag gccgagcgtg ggcttgatgc cgacgagcga gttggccgaa gcggggcaga
     6661 cgatggagcc gtcggtttcg gtgccgacac cgacggcggc gaggctggcg gcgacggccg
     6721 cgccagagcc ggagcttgag ccgcaggggt tgcggtcgag cacgtaaggg tttctggtct
     6781 gcccgccgcg cgcgctccag ccgctcgtcg aatgcgtcga gcggaagttg gcccactcgc
     6841 tcaggttcgt cttgccgaga aggacgcagc cggcggcgcg caggcgttcg acgatgaagg
     6901 cgtcctgctt cgggcgcgac tcgacgaggg cgagcgagcc ggcggtggtc agcatccggt
     6961 cggcggtgtc gatgttgtct ttgatgagaa cggggacgcc gtgcaggggg ccgcgcgtgc
     7021 gccccgcctt gcgctcgcgg tcgagggctt cggcgataga gatagcttcg gggttcaact
     7081 cgatcaccga gttgagcgcg gccccgcgcc ggtcaacgcg ctcgatgcgc tcaaggtatt
     7141 tttccgtcag ctccttcgcg ctccacttgc ccgagcgcat ccccgcctga agctccgcga
     7201 cggtcgtctc ttccaattcg aacgagtcgg cggcccgatg tgaagcgacg ttctcggcgt
     7261 tagcactgga acgagccgcc agaggagcga cgagggcgag cgcgccgccg actgcccccg
     7321 tcttcaaaaa gtccctacgg ctcagcggcg agcctccgcg cgcacccttc tgacggccca
     7381 tcacaatctc ctcgcgttga cgtttggcag attgtgaacg tgcgcgcgcc gacaatcaag
     7441 ctcccgtgag gctcagagaa tcgacggccg gcagcgcctt caggtcaggc tccacccacg
     7501 cgagccgcgc ctcccacgag aacagcagct cgcggctgta gtgcgtgagc ggggcgttgc
     7561 gctcagcgcg ctcgatgagc gtgttggcga gggaggcgag cgagcagccc tcgttgtagt
     7621 tggcttcgag gaagaagcgg acgtaatgca tccagaagag cgtgagcgtc tcgtggtagc
     7681 cgcgctcgcg cgtggtctcg acgccgttcg ccgcgttgta acgcttgatc cggcggcgca
     7741 cgcgctcgac cgcctccgcc cagtcgaagt ggagcaggtg ccagagcgcc accgtcaggt
     7801 gcgccgcgtg cgtccaatcg ctccacggga gcgtacacga cacgaagtcg cgcaccagcg
     7861 tctcgacctc ggcggctcgt tcgtagtgcg gtgcattgac gtgtgccact ggcataaaat
     7921 cgtctcctga aagacgcgta tcaaaagagt cgcggcgaca aagtttgatg aaacgtttac
     7981 gtgctctacc attgagctat ggcctccgta gttggagacc accgggactt gaacccggaa
     8041 ccccgtcatc taggatgtag ttccaccggc attcgccgcg aaaaagcttt tagcgttcag
     8101 cactccgcgg tcaggcagaa gtgataactg accgccgagt gccgaccgca tctcggggtg
     8161 acaaaaatga ggaagaccgg gcacggcctt tcgaccgccg gggaatcgcg tccccgcgta
     8221 ggcccatcgg ttggagcgat gtagtcctcc cgcgcattca ccccggagga gtgagcagtg
     8281 agcggtaagc ggtaagaagt ttttactact cactgcttac tgctcattgc tcacattctg
     8341 tcggcgacaa ggtttggtga gaggtcaccg gccgggcaag aattggccgg gacagggttc
     8401 gaacctgtaa ttaggctttc gccttttacc atgtactccg cacctgcatt cgccaacttc
     8461 aaagaaccgc gtttcaaatt cacggggccg acaaagattg ccgagacagc catgttatgg
     8521 gtggatgtag tccccgacgg cattcggccc ctcttgacag tgagcagtga gcagtaagca
     8581 gtgagcagta aaatctgaaa aactgcttac tgcttactgc tcactgctta cttcaaagag
     8641 ctacgccttc gatcacttcg acccagtggc cggcgtcgtg gcggccctgt gcgaagccgg
     8701 ccagcacgtc gaagacctgg tcggagaagc cgccgacgtt gaggatgtcc tcgcgctcga
     8761 cggcctgcgt cgtgccgtag ggctggaggt cgatgcagac catgcgcgcg cgcgggttgc
     8821 gcgccttgaa ctgcgcccac tcgcgcatcg tctccgtgtg gctgccgccg aagcggccgt
     8881 actgcggcgc gtccacccac gactcctgat cggagacgta gacgacgagg tcgccctcgg
     8941 ccttgcgctc gttgaggcgt cggagcgggg cgctacagtt cgtgccgccc gccggcaacg
     9001 ccgcgagctt ctgcgcgttg gtcatcacgc tgtcgcgcgg gttcagttgc accttcacca
     9061 cgtccgactc aaacgggata acctccgccg aagggttctt gcgcaggagc gtcgccgcca
     9121 cgagcgcggc cacgtcgatg caacgcaccg ccgaggtcga gccgcgacgt tggcccgtca
     9181 cggccgagtg catcgaaccc gaaacgtccg gcaggacgta gaccttgccg tcgatgcgcg
     9241 gcacgttcgt cgtcgccacc tccagcgcgt cctgaagcgc atcgcgcacc aactgcggca
     9301 cgcgctcgtc cgtcgagacg taggccgcga gaagctggta cgggaagaca cgcgccttgc
     9361 gtaccttttc ggggtcggcg agacgctgcg cgacaagctc ggccacgccc ggctgctcga
     9421 agacgccgtg gcgcgcgaac gtgttgaggt tcatgcgcgt catctgccac ggggcgcggc
     9481 gcgcaatcgc cgtccagtcc gacgtgccca gcggcagcgc cgtgagcatc tggaatggaa
     9541 cgtccggcac gtcgagcgtg tcgccggcct tgaacgcctc gaactgctgc acgaggagcg
     9601 gcaggcggcg ggcgtcgtgc tcgcggccca cgaggtagcc gaagagcgcc tcgcgctccg
     9661 gcgtgcgcgg gcgcgggtga accatgcgga cgatgtcggc gagcgaaggc tgttggccga
     9721 ccgagccggc gaacaccgcc gcgtcgtcgc gcg
//
LOCUS       DASVES010000103         9529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_407781, whole genome shotgun sequence.
ACCESSION   DASVES010000103 DASVES010000000
VERSION     DASVES010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407781"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..2997)
                     /locus_tag="VF240_03705"
     CDS             complement(268..2997)
                     /locus_tag="VF240_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7174373.1"
                     /translation="MSVKGRSVWVLIVFASLSLIQFTTVEAQGNSNGVVSGVVHDASG
                     AVVTGAEVSLVNAQQRQFGLTTTDAEGRFRLGGVPAGSYVLSISRQDFSRRREAVQLA
                     SGASVEVDIVLEVNQVAEDVTVTAETGLAEDKDRIPQAVNVIPEDSLRQRTTAVLAQV
                     ADEEVGASLQRTSPTVGAVLVRGLTEVGVYVDGVRYTNSTQRGGINTFFNLLDPTSLR
                     AVEIQRGPNTAQYGSDGLGGTVQLVSRQPEFGFDPAQLHGEFNTFFNSADLSFGGNTL
                     LTYGTKRFGILTNVAGRRVNTLRPGRGIDSHSAVTRFLGLPSDIIGETRLPDTAFTQY
                     GGSLHLTYALAADQQLTFRYQRAQQDGGKRYDQLLGGDGNLVADLRNLMLDFGYLRYF
                     KQGFGFFDNLSATVSYNSQREERVNQGGQGNPLAGITHDKERTSQLGFGFYLDKQLPH
                     RNSFLIGGDVYRDHVDAPSYTFEPSTGLTTLTRPRVPHGARYILAGLYVQNAFEAVPD
                     RLRVSGALRYNVGSYRARATDAPLPGLFPDDSLRVDDFSGRIGAVATPFDGFNIAFNF
                     SRGFRAPNITALGSVGLVGVGFQVAASEVANLNATVGTTAGADAVSSGVPVAPLLSET
                     SKNVDFSLRFRRGRFDTELTAFVIDYADSIVRQTLILPPGAVGLRLGSQRVERQDANG
                     AVFVEASASPVLVQVNFGDTRMKGIEYEVDYRLTNAWSFRGNYSYVHAADRATGDAPN
                     LGGGGVPPQTGFLSVRYQPPARRYWVEAYTTLAGRQSRLSSLDLSDRRTGAVRSRTNI
                     QNFFRRGACVRGLTTRGSTGQCGSAGGTLIATGETLAQVQNRVLGPGVDSAPLFSAIP
                     GYALFNVRGGFRLGERQEVTLDFENIGDKSYRGPGWGVDGPGRSITVRYRMSF"
     gene            complement(3123..3196)
                     /locus_tag="VF240_03710"
     tRNA            complement(3123..3196)
                     /locus_tag="VF240_03710"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3160..3162),aa:His,seq:gtg)
     gene            complement(3322..3394)
                     /locus_tag="VF240_03715"
     tRNA            complement(3322..3394)
                     /locus_tag="VF240_03715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3359..3361),aa:Arg,seq:tct)
     gene            3547..4683
                     /locus_tag="VF240_03720"
     CDS             3547..4683
                     /locus_tag="VF240_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7174374.1"
                     /translation="MPAQEESKRNIQARWIALLFVTAVALYLCWLMLQPFLDVLLWAL
                     VLVIIFYPVHRRILARTGKPSLSALLSSVLVIVTILVPLSLVTLAVVREVAQLAQYLQ
                     ANADTLLDPNSPQLSGLMSWLGQYIDVEQLRSQDFIAERLKNLSGTIAGRTLGFLGGF
                     VGGLVQTFFVIFTMYYLFRDGERMRSALRFALPLERSQSNEIFHRTNEVIGASVYGVL
                     VIATIQGTVGGLAFWLLGLPSALLWGAVMILLSMIPMLGAFLVWVPAALYLAVTGHWV
                     KALILTLVGLFVIGTIDNFLRPKLVGEKTRLHELIIFFSVIGGLHVFGIIGLVLGPVI
                     VAITLALLDVFRKADDPPHATTDEKTLLEEQEDLREVPPQPETG"
     gene            complement(4778..5017)
                     /locus_tag="VF240_03725"
     CDS             complement(4778..5017)
                     /locus_tag="VF240_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174375.1"
                     /translation="MNFTTVTLEDRLQAIDHIQARRYSKLAGGPLDIATEGILRHLRA
                     CDRMDVNPDVAAVREIIDDALNGRRVFAETFDNAA"
     gene            complement(5526..5837)
                     /locus_tag="VF240_03730"
     CDS             complement(5526..5837)
                     /locus_tag="VF240_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7174376.1"
                     /translation="MATSKAGKKRMSQSEVYNHFAEKTGMKRAQVKEFFEELSNLAMG
                     EVKGSGEFVLPGFGKLVLSERKAREGRNPQTGETIQIPAKTTLKFRVGKGMKDNVVPK
                     K"
     gene            6425..7636
                     /locus_tag="VF240_03735"
     CDS             6425..7636
                     /locus_tag="VF240_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX7174377.1"
                     /translation="MPRERSGSFFKREKKLENGKTVVTWWARLTYVDHVTGKRRDRQR
                     RAENRAHAKELVQKLIAEYDATGGRSLEHGRKTFAELAEHYEATYLKPAVYVEGRKVA
                     GVRSLTPAKAAVSALKAYFGVRPLRGITYGDILEYRAARLRTPTRGDLARHERELQED
                     PQVKVRVTRTIAAVNRELEKLRHMLNIAQREGWILRNPMQGGDPLVSKADEKKRERII
                     TRDEEAKLLEACEGRRAHLRPIIVCALDTGMRRGEIITLRWRDVDFDNRVITIQAFNT
                     KTMRERQVSMTTRLALELERLYEVSPKRPDILVFGVVNNVKRSFTTVRRAAGLDDLRF
                     HDLRHTAATRLVGAHIPLSEVGRVLGHTQANTTYRYVNANIETARRAAAALDAYHSGN
                     ETEEPNAETVN"
     gene            8041..9027
                     /locus_tag="VF240_03740"
     CDS             8041..9027
                     /locus_tag="VF240_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174378.1"
                     /translation="MIDESEIHELGEKIKSSVLITAEREPSNFEPETPFWVVRSDPLP
                     PEIVRQYGLLPQTVTLQYRLDAGTAGMRLHFFMEGFAQEVIYECDGILDKFIAGTTVL
                     DAEGESHNLSDVVSPALHKLLLETLTEHTVERFIYALGNKVEDAADELLGDCVLVALA
                     AFLEECKEFSDLGFASFNTEFISAEYNRITKQAAAERKASLKGTLKYILSPNLDELEE
                     HYRSVLPMWKDAKTLYKQNRNRETWRDIVRAAQPDLKDYDDLISRLSGRLNDLPENIQ
                     AKLSEKGGDSKPSSIALEHAARLCGSEPYEYSVRYLYIKLRKCRNGEQDAYS"
BASE COUNT         2106 a         2646 c         2921 g         1856 t
ORIGIN      
        1 gcaggggcgc gtcgtcgcgt cggccgagcg cggcgcgtgt gtcgcaccag cgcgggccgg
       61 caaacaaggc ggccgtcccc gccgaaaaaa tttacgcggc ccggtgtgtg gcgacgcgcg
      121 tctgttaagt aaaaaacccg aaatgttcag gtgaaggcaa gcgcccctgg gagcggagcg
      181 cagagataat aaacgttcgc ggcgcaagcg gtgattgtgt tctgattaca atcccgttgc
      241 gccgcgaact atttgagcga gggctggcta gaagctcatg cggtagcgga cggtgatgct
      301 gcggcccggc ccgtccacgc cccagccggg gccgcgatag ctcttatcgc cgatgttctc
      361 gaagtcgagc gtgacctcct gtctctcacc gagacggaat ccgccgcgca cgttgaagag
      421 cgcgtagccg gggatggccg aaaagagcgg ggcggaatcg acgccggggc cgagcacgcg
      481 gttctgtacc tgcgcgagcg tttcgccggt ggcgatgagc gtgccgccgg ccgagccgca
      541 ctgacccgtc gagccacgag tcgtcagccc gcggacgcac gcgccacggc ggaagaagtt
      601 ctggatgttc gtgcgcgagc gcaccgcgcc cgtgcgccgg tcgctgaggt cgagcgagga
      661 gagacgcgac tgcctgccgg ccagcgttgt gtaagcctcg acccagtagc ggcgggcggg
      721 cggctgatag cgaacactca ggaagcccgt ctgaggggga acccccccgc cgccgaggtt
      781 gggcgcatcg cccgtcgcgc ggtcggcggc gtggacgtaa gagtaattgc ctcggaagct
      841 ccacgcgttc gttagccggt aatcaacctc gtactcgatg cccttcatgc gcgtgtcgcc
      901 gaagttgacc tgcacgagca cgggcgaggc ggaagcctca acgaagaccg cgccgttggc
      961 gtcctgccgc tcaacgcgct gactaccgag ccgcaagccc acagcgcccg gcggcagaat
     1021 gagcgtctgg cgcacgatgg agtcggcgta gtcgatgacg aaggccgtca actccgtatc
     1081 gaagcgcccg cgtcggaagc gtaaactgaa atcgacgttc ttgcttgtct cggagaggag
     1141 cggcgcgacg gggacgccgg acgagacggc gtccgcgccg gcggtcgtcc cgacggtggc
     1201 attgaggttg gcgacttcac tggccgcgac ctggaagccg acgccgacga ggccgaccga
     1261 gccgagggcc gtgatgttcg gcgcgcggaa gccgcggctg aagttgaagg cgatgttgaa
     1321 cccgtcgaac ggcgtggcga cggcgccgat gcgtcccgaa aagtcatcga cacgcagaga
     1381 gtcgtcgggg aagaggccgg gcagcggggc atccgtcgcg cgcgcgcggt aggagccgac
     1441 gttgtagcgc agcgcgccgc tcacgcgcag gcggtcgggc acggcctcga aggcgttctg
     1501 cacgtagagt ccggcgagga tgtagcgcgc gccgtgtggg acgcgggggc gcgtcagcgt
     1561 cgttaagccg gttgaaggct cgaaggtgta agacggggcg tcaacgtgat cgcggtaaac
     1621 gtcgccgccg atgagaaaac tgtttcggtg cgggagctgt ttgtcgaggt agaagccgaa
     1681 gccgagttgg ctcgtgcgct ccttgtcgtg cgtgatgccg gcgagcgggt tgccctgacc
     1741 gccctgattg acgcgctcct cgcgctggct gttgtaggag acggtggccg agaggttgtc
     1801 gaagaagccg aagccctgct tgaagtagcg caggtagccg aagtcgagca tcaggttgcg
     1861 gaggtcggcc acaaggttgc cgtcgccgcc gagcaactgg tcgtaacgct tgccgccgtc
     1921 ctgctgcgcg cgctggtagc gaaacgtgag ttgctgatcc gcggcgagcg cgtaagtcag
     1981 gtggagcgag ccgccgtact gtgtgaatgc cgtgtccggg agccgcgtct cgccgatgat
     2041 gtcggacggc agcccgagga agcgcgtgac ggccgagtgg ctgtcgatgc cgcgacccgg
     2101 tcggagcgta ttgacgcggc ggccggcgac gtttgtcagg ataccgaagc gcttcgtccc
     2161 gtaggtcagc agggtgttgc cgccgaagga gaggtctgcg ctattgaaga aagtgttgaa
     2221 ctcgccgtga agttgcgccg ggtcgaagcc gaactcgggt tggcgcgaga ccaactgcac
     2281 ggtgccgccg aggccgtcgg agccgtactg tgcggtgttc ggcccgcgct ggatttcgac
     2341 ggcgcgcagg ctcgtcgggt cgagcaggtt gaaaaaggtg ttgatgccgc cgcgctgcgt
     2401 cgagttggtg tagcgcacgc cgtcaacgta gacgcccacc tcggtcagcc cgcgcaccag
     2461 cacggccccg acggtcgggc ttgtccgctg gagcgaagcg ccgacctcct cgtcggccac
     2521 ctgcgcgagc acggcggtcg tgcgctgtcg cagcgagtcc tccgggatca cgttcaccgc
     2581 ctgcggaatt ctgtccttgt cctcggccag tccggtctcg gccgtgaccg tcacgtcctc
     2641 ggcgacctga ttgacttcaa gaacaatgtc cacctccacg ctcgcgcccg aagccaactg
     2701 cacggcctcg cgccgccggc tgaagtcctg ccgcgaaatc gacaacacat aggagccagc
     2761 gggcacgccg cccaggcgga atctgccctc ggcgtcggtc gtcgtcaggc cgaactgccg
     2821 ctgctgcgcg ttgactaatg aaacctcagc gccggtgacg accgcgcccg acgcgtcgtg
     2881 gacgacaccc gacacgacgc cgttcgagtt gccctgagct tcaactgtgg tgaattgaat
     2941 gagggacagg gaagcgaaga ctatgaggac ccaaacgctg cggcctttaa cactcatgcc
     3001 ggtcaactcc gaaaatccga ttgcgcgaac agagggcttg taagggcaga cttacaaaaa
     3061 gagttggcgg acgagaacgg acaaacagcg accgatctcg tccgccacgc ggaatcaaag
     3121 agggcggcta gcgggtctcg aacccgcgac ctcctaatcc acagtcaggc gctctaacca
     3181 actgagctat agccgccaca aaaaccgaac ctgcgagacg cgaccaatag tagccaagcc
     3241 ttcgcccgat gacaagggca tgcaggccgt gagcgcgtca ccggaatcga agcgaaaagg
     3301 gtcggcgccg cggcacggca atacccccgg caggactcga acctgcgacc ctctgcttag
     3361 aaggcagatg ctctatccac tgagctacgg gagccgaaaa attcgacgcg gaacggatgt
     3421 taacacgcgt cggacgggaa tcgccaaacg ggcaagtctg acacttgcaa cggcgggcgg
     3481 cgggcagttc ggagtgaccc aaaaagcggg acggcgagcg gacgtgctgt gataaagtgt
     3541 gaggccatgc ccgcccaaga agagagtaag agaaacattc aggcgcgttg gatcgcgttg
     3601 cttttcgtca ccgccgtcgc gctctacctg tgctggctga tgctccagcc tttcctcgac
     3661 gtgctcctgt gggcgctcgt gctcgtcatc atcttctacc ccgtccaccg gcgcatcctc
     3721 gcgcggacgg gcaaaccctc tctgagtgcc ctcctctcaa gcgtgctggt gatcgtcacg
     3781 atcctcgtcc ccctgagcct cgtgacgctc gccgtcgtgc gcgaggtggc gcagctcgcg
     3841 caatacctac aggcgaacgc cgacacgcta ctcgacccaa actccccgca acttagcgga
     3901 ctgatgtcgt ggctcggcca gtacatcgac gtggaacagt tgcgctctca ggatttcatc
     3961 gccgagcggc tcaagaacct gagcgggacg attgcggggc gaacgctcgg cttcctcggc
     4021 ggcttcgtcg gcggcctcgt ccagacgttc ttcgtcatct tcacgatgta ctacctcttc
     4081 cgcgacggcg agcggatgag gagcgcgctg aggttcgcgc tgccgctcga aaggtcgcag
     4141 tcgaacgaaa tctttcatcg gacgaacgag gtgatcggcg caagcgtcta cggcgtgctc
     4201 gtcatcgcga cgatacaggg aaccgtcggc gggctggcct tctggttgct cgggctgcct
     4261 tcggcgctgc tgtggggcgc tgtgatgatt ctcctgtcga tgatcccgat gctcggcgct
     4321 ttcctcgtct gggttccggc agccctctac ctcgcggtga ccggccactg ggtgaaggcg
     4381 ctgatcctca ccctcgtcgg cctcttcgtc atcggcacca tcgacaactt cctgcgcccc
     4441 aagctcgtcg gcgagaagac gcgtctgcac gaactcatca tcttcttctc cgtcatcggg
     4501 ggcctgcacg tcttcggcat catcggcctc gtgctcggcc ccgtcatcgt ggctatcacg
     4561 ctcgcgctcc tcgacgtttt caggaaggcc gacgacccgc cgcacgccac aaccgatgag
     4621 aagacgctcc tcgaagaaca ggaagacctg cgagaggtgc ctccccagcc tgagacggga
     4681 tgacgggaaa taaaaagaag aggctgcccg ttagcagggc agcctcttct gaatgaattc
     4741 aaacagaaag gaaaagcgcg cggcagtcga aaccggctta cgcggcgttg tcgaaggtct
     4801 cggcgaaaac acggcggccg ttgagcgcgt cgtcgatgat ctcgcgcacg gccgcgacat
     4861 cagggttgac atccatgcgg tcgcaggcgc gcaggtggcg caaaatgccc tcggtggcga
     4921 tatccagcgg gccgcccgcc agcttggaat agcggcgcgc ctggatgtgg tcgattgctt
     4981 gcagcctatc ttccagagta acggttgtaa agttcacttt ctcccagctc cttatttttc
     5041 cagcccgccg aaatagttgg gggcgaatta agtttaacgt tggcctgact ttagatgacg
     5101 aagcatccgg aggggatgcg tttacgttgc attcccgaaa ttttttacgc aaccttcccc
     5161 gcgttactga ccacctgtcg gcgacttgcg aaggggtgct tgaaaggagg cattttaact
     5221 aaacccggta aaaaatacta gaaaaatttt cgatggcagt aactagttga ttacacgtag
     5281 gttcgccgca gattgccgca gccggagaag gggactccaa agactgggcc tcgatgtgat
     5341 gtcgcgacaa tcggagttgg ttatgttacg ggggaggcgc cgaaacgcgc tgatatctat
     5401 attaagtcta ctgatataaa gaattaccgc ctgagattcc aactcgcccg cgggcagaca
     5461 gagttggaac attcaggcgg taatcgcagc ttatggcctt ggaaaatgac ggacgagtga
     5521 actgtctact ttttcgggac cacgttatct ttcatgcctt tgccgacgcg gaacttgagc
     5581 gtcgttttgg ctggaatctg gatggtctcg cccgtctgag ggttgcgacc ctcccgcgcc
     5641 tttcgctcgg agagcaccag ctttccgaag cccgggagga caaactcgcc gctgccctta
     5701 acctcaccca tcgcgaggtt tgacagttcc tcgaagaatt ctttcacttg cgcgcgcttc
     5761 atcccggtct tttccgcgaa gtgattgtag acctctgact gagacatgcg cttcttacct
     5821 gctttggacg tcgccatggc gttatcttaa ttcctcctgc aaatcgtgaa aattccgaaa
     5881 gagcttgcca agcacatata tcacacggga gtcattaagt ctcgaaaaaa aagcgaaaaa
     5941 cggcgctttt tttatgattc gggatgaagt ggctgactcg acaccgccgg gcggacaaaa
     6001 tatccggccg gggcgaggcg aaaactgtcc gccttttgaa attcaaacag ggcggagagt
     6061 gagagttttc agcgagaggg aatagtgaag gcgaatactt aataaacccg aagggcgttt
     6121 acctgctaat ggttagccgg cacgtggccc gctcagcaaa caatcaaaac gcggattgta
     6181 cgagcagatt gcaatcgggg cggcaggatt cgaacctgcg acctctcggt cccaaaccga
     6241 tgcgagggca ttatcaggag ttatccaagt gattcacaat ccacacacac cgcatgttta
     6301 ttatgtttcc gttgatggca gcggttcatg cggttctgat gtaggctcgt actagcatca
     6361 agtatgtgtg tgaatgtgtg tgcggcggcg gcaaatatat tacagattga gaggctgaaa
     6421 acccatgcca agagaacgat ctggctcatt cttcaaacgt gaaaagaaac ttgagaacgg
     6481 taaaacggtc gttacgtggt gggcgcgtct cacttatgtt gaccatgtga ccggcaagcg
     6541 ccgcgaccga cagcgccggg cggagaaccg cgcgcacgcg aaagagttgg tgcaaaagct
     6601 tattgcggag tacgacgcga cgggcgggcg ctcgctggaa cacgggcgca agactttcgc
     6661 tgagcttgca gagcattacg aagccactta cctcaaaccc gccgtgtacg ttgaagggcg
     6721 caaagtcgcg ggagtgcgaa gcctcacgcc ggcaaaggcg gctgtgagcg cccttaaagc
     6781 ctatttcggc gtgcgtccat tacgcggcat cacttacggc gacatactcg aataccgggc
     6841 ggcgaggctc aggacgccga cacgtggcga cctcgcccgg cacgagcgcg agttgcagga
     6901 agacccgcaa gtaaaggttc gcgtcacccg caccatcgcg gccgtgaacc gcgagcttga
     6961 gaagttgcgt cacatgctga acatcgcgca gcgcgagggc tggattctga gaaatccgat
     7021 gcagggcggc gatcctctcg tcagcaaggc cgacgaaaag aaacgcgagc gaataatcac
     7081 gcgcgacgaa gaggcaaagc ttttagaggc atgtgagggg cggcgggcac accttcgccc
     7141 gatcatcgtc tgcgcgctcg acaccggcat gaggcgcgga gagatcatca cgctgcgctg
     7201 gcgcgacgtg gattttgata accgtgtaat aacaattcag gcattcaata ccaagaccat
     7261 gcgcgagcgc caggtatcca tgacgacgcg cttggcgttg gagctagaaa ggctttacga
     7321 ggtgtcgcca aagcgcccgg acattcttgt gtttggcgtc gtcaataatg tgaagcgcag
     7381 ctttaccact gtgcggcgtg cggctggcct agatgattta cgcttccatg accttcggca
     7441 cacagcggcc acgcggctcg tcggcgcgca catcccgctt tcggaagtcg ggcgcgtctt
     7501 aggccacacg caggcaaaca ctacttatcg ctatgtcaac gcgaatatcg agacggcccg
     7561 gcgcgcggca gcggccttag acgcatatca ctctggaaac gaaacggagg agccaaacgc
     7621 ggagactgta aattagtaat gttccctccg ggctgttcgc atttttagct tattgaggtg
     7681 agtaataaag ataatcggcc ttgctaaaat ccttgcagtt tcgtcagatg aaaactccgg
     7741 tcaatcttta gaaaaaacgc gttgacggaa gctgtcattt atgtagaatg acgcgcgctt
     7801 gatgtgttta tacccttcaa aatctccccc catgctggat agttagagcg tgagacggca
     7861 ccacgccgca cacccttttc aaactaaaca gtgaagtaaa agctcaaaaa attgagcatc
     7921 acatctcgcc tttcaaggga aggcgcttct tatttcccaa cacagattag tacatctagg
     7981 gcttccgtta agatagttgt gccacagtct aatacgggga gactcatggg gtcggatgcg
     8041 atgatagatg agagcgaaat tcacgagctt ggcgagaaaa taaagagcag cgtcttgatt
     8101 actgctgaaa gggaaccaag taactttgaa ccggaaacgc cattctgggt ggtgaggtcg
     8161 gatccgttgc cacctgaaat agtgcgacag tatggcctac taccccaaac tgttacgttg
     8221 caatatcgtc tcgatgccgg aacggcggga atgcgtttgc atttttttat ggagggcttc
     8281 gcgcaagagg tgatatatga gtgcgatgga atactcgata aatttattgc cggcactaca
     8341 gtcctagacg cggagggtga atcgcataat ctaagcgatg ttgtttcacc cgcgttgcac
     8401 aaattactac tagaaacact cactgagcac acagttgaga gatttattta tgctctgggg
     8461 aataaggtag aggatgcggc ggatgaactc ttaggcgatt gtgtcttagt cgccctagcc
     8521 gcctttcttg aagagtgtaa agagttcagt gatctaggat tcgcctcttt taatacggag
     8581 ttcattagcg ccgagtataa tcgaatcact aaacaagcgg cagcggagag aaaggcgtct
     8641 ctaaaaggga cattgaagta tattctgtca ccaaacttgg acgaacttga agagcattat
     8701 cgtagcgtcc taccaatgtg gaaggacgcg aaaacccttt ataaacagaa tcgaaatcgt
     8761 gaaacttggc gagatatagt cagggcggcg caaccagatt tgaaggatta tgatgatcta
     8821 atatcgcggc tctcggggag gctaaacgat ctcccagaaa acatccaagc aaagctctca
     8881 gagaagggtg gcgactctaa gccgtcgagt attgcccttg aacatgcggc aaggttgtgc
     8941 ggttcagaac cctatgagta cagcgttcgc tacctgtata tcaagcttag aaaatgtagg
     9001 aatggtgaac aggatgcata tagctaattt caccgaaagg tattccagcg gtgaacattt
     9061 cagacgttga atagctcacc gacgaaccac cattatgctg agctaaaaaa acttaataaa
     9121 gtgcaagagt ttttcacctc ggggtgcata ctgaagccac tgctaacggc agtggctttt
     9181 agctttatgg agaaaggaat gaaaacgtga gtaatacaga aagtgatgga acagggctga
     9241 tgtctgaagc agaggccgcg cggaggtgtg gcgtctcacg catcacattg ctacgaatgc
     9301 gaaaggccgg gaggataggc ttttatagaa taggcacaag gattctgttc tctgaatccc
     9361 atatcagcga ttttcttagc cgtgtagaac gtaaagccca atcgcagaaa ggcgctgcgt
     9421 gattccgcac gacgttatca atctcctgca aggcgtcagg cgtagcggcg agggctggac
     9481 ggccagatgc ccggcacatg atgacgaaca caatagccta agcattggc
//
LOCUS       DASVES010000104         8816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_350818, whole genome shotgun sequence.
ACCESSION   DASVES010000104 DASVES010000000
VERSION     DASVES010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350818"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..943)
                     /locus_tag="VF240_03745"
     CDS             complement(125..943)
                     /locus_tag="VF240_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7174379.1"
                     /translation="MERVLRQTWAFFFRDYHLTRRYMSWVIVYTFYAVVNSATIALIG
                     VAAGDRRLTLTLLVGVLLWNFLSVLFQEISNSITYERWEGTLEYTFMAPVSRMTHLAG
                     VSLFTLAYSVVRTIIIVAGLSLIIDLDLARANFAGLFVVLMVSSMAFVGLGLMAAVLP
                     VMSPERGAEATNIFQGILLLVSGVYYPVEVLPGWLQPLAAISPATYALRASRKLLGIE
                     GAGEGPQAGAPLSEVVPELLVLAVMGAAMIPLGLYVFGLVETWAKRTGKLKRTG"
     gene            complement(943..1869)
                     /locus_tag="VF240_03750"
     CDS             complement(943..1869)
                     /locus_tag="VF240_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7174380.1"
                     /translation="MSNLGIEVRNLHKHFSKRKGFLKGRKTVHALRDISFEVRLGETY
                     GLLGPNGSGKSTLIRVLATLLLPDSGYVRLLGHRIPGEEAEVRRKIGRVSVDAAFYKK
                     LSARENLLYSAFLYGMDKPTAERRAQEILEALGLEQEKFTDPLEEMSRGMQQKVAITR
                     ALLVNPPLLLLDEPTTGLDPKSKRDVQTFIERLRREEGTTILLTSHDMAETERLCSRI
                     GFLAHGRLIAEGTATELKQKAGADSLEDAFIALAGEKLDADEKNDGDKGGEGDKGNAN
                     GQSEAAKVVGAVENGDGAGVADESGAEVLESK"
     gene            2158..3387
                     /locus_tag="VF240_03755"
     CDS             2158..3387
                     /locus_tag="VF240_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174381.1"
                     /translation="MMKTVLLILFIAAACVSTAAQDEALQFKMPPVPERAAAPEGFVP
                     KDWRLLGKAAGDLNGDGRADAAVVAAHDAEGSGPEDVWEEPRLLVIALGEESGGLRLS
                     VASDAVVLCRGCGGVFGDPFEAIDVERGTLVIRHYSGSRDRWGFIDRFRLQDGRWTHI
                     GATERHTDTLDCSYFEERDANLITGLVIESGKMGRSSYNRTFYELRAALIERAPAVDG
                     AASAGEWGDPHVVRLAAKEQVAVGTALWRGADDASARLSASWRGGELYLRAEVADDSV
                     TPGDAVRLISKTGQVVRPVEMKSAPREGGYAVEARYTLKSLGLEELEAQIRELRGYAA
                     DKDISGDLPLRVSVEVIDEDAGQKTASVLSTSRGGRKYPARVRLTRHAGLPLLSDFDR
                     ESSGDVLPPPGFGVARP"
     gene            complement(3515..4843)
                     /locus_tag="VF240_03760"
     CDS             complement(3515..4843)
                     /locus_tag="VF240_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7174382.1"
                     /translation="MRESNRSKISVNRRRFLSAVGQGVGLATLASPAVASLLGDVEAA
                     SRRVAHLTPEEAARDEDFWFSIQQAFTVTRGIVNLNNGGVSPSPRIVTEALVRYIWQQ
                     EDATAYTMWQILEPQSETIRTGLAELFGCDREEIAITRNASESLEALLFGFDFKPGDE
                     ILSTTQDYPRMLTTLRQRERRDGVRLKLVKIPLAPKNPSEITAAFERAVTPRTRLLLV
                     SHQINITGQITPVREVCEMARARGIETVVDGAHSFAQFDFSRDRLSCDYFASSLHKWL
                     YAPKGAGLLYVKRDKIAKVWPLMAADGKQDTDIRKFEEIGTHSAAPRLAIGEAILFHR
                     GLGPARKEARLRFLARHWMNQLKDVPRVRFHTSFDERQSCAIATVEIEGVEPGALGAH
                     LMGAHKIFTTPIVHEEFRGLRITPNVYTTLNELDRFCEVMKRVATKGLPT"
     gene            complement(4863..7016)
                     /locus_tag="VF240_03765"
     CDS             complement(4863..7016)
                     /locus_tag="VF240_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEX7174383.1"
                     /translation="MRKILLIVFLLLILSLCASAQTVQSPNGKLSLVFSLTADGEPAY
                     ELSFAGKSVVRKSRLGMELKDQPALTKGFTVAKSDTAVTDETWETVWGEERRIRNNYR
                     ELAVTLRQPGPKERTLILRFRLFDDGLGFRYEFPEQEHLRYFTVTDERTEFNLAGDHK
                     TFWIPGDYDTNEYAYSTTRLSEIDAERGKASQEIATRTFIAANLVQTPLMMKTADGLY
                     INLHEAALVGYPGMLLSVNRQTFGLTSYLTPDPVGNKAYLQAPARTPWRTVVVSDRAG
                     QILSSRLILNLNEPSKIADTSWIKPIKYVGIWWEMHVPNKSSWNYADTGNIKLSETDW
                     SKLKPNGRHGATTARTRRYIDFAAAHGFSGVLVEGWNVGWEDWFGNWKEEVFDFVTPY
                     PDFNVEELHTYAASKGVKLIMHHETSSSVTNYERRMDDAYRFMKRHGYDAVKTGYVGR
                     IIPRGEWHDGQWMVNHFVRVAEKTAAYKIMLNAHESVRPTGLHRTYPNWLANEAARGN
                     EFNAWSAGNPPDHETILPFTRLMGGPMDYTPGIFQIKMDYYQPGKKEQVHTTLAKQLA
                     LYVTLYSPLQMAADLPENYEKHLDAFQFIKDVGVDWDETRILEAEPGDYLSVARRARG
                     REEWYVGAITDEEARTASVPLSHLAAGRRYVATVYADADDAHWERNPMAYKIQSYLVD
                     NRTVLKLKLAPGGGAAVSLKPATAEDMRKLKPYAD"
     gene            complement(7275..>8816)
                     /locus_tag="VF240_03770"
     CDS             complement(7275..>8816)
                     /locus_tag="VF240_03770"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7174384.1"
                     /translation="RGRLVTPGFNDAHIHFLNGGLALLRVELNGAPTLAEAKRRVAER
                     VRAARPGEWILGRGWDHTLWGGEWPTKKDLDEVAPANPVFLTRVDGHVSWANSVALRE
                     AKLTRETRAPEGGEILHDSAGEPTGIVKETAQGLVGRVIPAPTDEQNMEALERALAEA
                     RRYGITSIQDNSGYRTTRLYRELLKRGRLTVRVSEWQDFSDTVAELKRQRAEFAAFKD
                     DPLVLRLGALKGVMDGTLGSRTAAMITPYSDDPSNSGLPRRPAEEVTRMIVERDAAGF
                     QITLHAIGDRANRMALDGFDAARQSREKNGTQMGTLRVEYTIRTTPPGSSERVVSTGI
                     LSPRHRIEHAQVVAPTDFARFRDLGVVASMQPAHAISDKRWAQARVGEYRVLGAYSWH
                     TMKAHGVPVAFGTDWPVEPINPYLGLYAAVTRQSAEGADPAGGWWPQERLSIEDAIRY
                     YTTGSAYASFDEREKGQIAAGMLADLVVHTRDLLTVKPEEILQTETAITVFNGRVVHE
                     AAARP"
BASE COUNT         1429 a         2765 c         3013 g         1609 t
ORIGIN      
        1 cgcacctgct cgtactgccg aagctcaact aacggcagaa agggtaaggg gtaaagggga
       61 aggggtaagg gaaagacaac ttcttttccc ttaccccttc ccctttaccc cttaccctct
      121 tccttcaccc cgtgcgcttg agcttgcccg tgcgcttggc ccacgtctcg acgaggccga
      181 agacgtagag gccgagcggg atcatggccg cgcccatcac cgcgagcacg aggagttccg
      241 gcacgacttc cgagagcggc gcgccggctt gcggcccctc gcccgcgccc tcgatgccga
      301 gcagcttgcg gctggcgcgc agggcgtagg tcgccggcga gatggccgcc agcggctgga
      361 gccagccggg caacacctcg acggggtagt agacgcccga gacgagcagc agtatcccct
      421 gaaagatgtt cgtcgcctcg gccccgcgct cgggactcat cacgggcagc accgccgcca
      481 tcagacccag tccgacgaag gccatgctgc tcaccatgag caccacgaag agaccggcga
      541 agttggcccg cgcaaggtct aagtcgatga tgagcgacag acccgcgacg atgatgatcg
      601 tgcggacgac gctgtaggcg agcgtgaaca ggctcacgcc cgccagatgc gtcatgcgcg
      661 agaccggcgc catgaaggtg tactccagcg tcccctccca acgctcgtag gtgatcgagt
      721 tcgatatctc ctgaaagagc accgagagga aattccacaa gagcacgccg acgaggagcg
      781 tcagcgtcag gcgacggtcg ccggccgcca cgccgatgag cgcgatggtc gccgagttga
      841 cgaccgcgta gaaggtgtag acgatcaccc acgacatata ccgccgcgtc aggtggtagt
      901 cgcggaagaa gaaggcccac gtctgtctca ataccctttc catcacttac tctccaaaac
      961 ttccgcgccg ctctcgtcag cgacgcccgc accgtctccg ttctcgacgg cgccgacaac
     1021 tttagcggcc tcgctttggc cgttcgcgtt gcccttgtcg ccttcgccgc ccttatcgcc
     1081 atcgttcttc tcgtcggcgt cgagcttctc accggcgagg gcgatgaagg cgtcttcgag
     1141 cgagtcggcg ccggccttct gcttcagctc cgtagccgtg ccctcggcga tcagtcggcc
     1201 gtgggcgagg aagccgatgc gcgagcagag gcgttcggtc tcggccatgt cgtgcgaggt
     1261 cagaaggatg gtcgtcccct cctcgcggcg caggcgctcg atgaaggtct gcacgtcgcg
     1321 cttgctcttc gggtcgaggc ccgtcgtcgg ctcgtcgagc aggaggagcg gcgggttgac
     1381 cagaagcgcg cgtgtgatgg cgaccttctg ctgcatcccg cggctcatct cttcgagcgg
     1441 gtcggtaaac ttctcctgct ccagacctaa cgcttcgaga atctcctgcg cgcgccgctc
     1501 ggccgtgggc ttgtccatgc cgtagaggaa ggccgagtag aggaggtttt cgcgcgccga
     1561 gagcttcttg tagaaggcgg cgtccacact gacgcgcccg atcttgcggc gcacctcggc
     1621 ctcctcgccg gggatgcggt ggccgagtag gcgcacgtac cccgagtcgg gcaaaagcaa
     1681 ggtggcgagc acgcggatga gcgtggactt gcccgagccg ttggggccga gcagcccgta
     1741 agtctcgccc aagcgtacct cgaaggagat gtctcggagc gcgtggacgg tcttgcggcc
     1801 cttcaggaaa cctttacgct tgctgaaatg tttgtgaaga ttacggactt ctatcccgag
     1861 attcgacatc gcaattcacc acgaaggctt gtgtaggacg gtatcggctt tccgaagcgg
     1921 gaaagtgtgg acgcgggcgc ggttggtggg gccgttaggc tctattgcga atcatcggta
     1981 tcaccttgcc gggtaggata cgtggggcgg cgcgactttg caaggttttc cgcgcgtcgc
     2041 ccggtcgctt gctgaagacg ttgatgttaa aaagactctc gcgctgatga cgttttcgca
     2101 taagattgcg gtgaactgac ttgcgctaca ctcatgtcac gagtgcccaa agaggtgatg
     2161 atgaaaactg tccttctcat attattcatt gcggccgcgt gcgtttccac agcggcgcag
     2221 gacgaagcat tacagttcaa gatgccgccc gtccccgaac gcgccgccgc gcccgagggc
     2281 ttcgtgccga aggattggcg gctgctgggg aaggccgccg gcgacctcaa cggcgacggg
     2341 cgcgcggacg ccgccgtcgt cgccgcgcac gatgcggaag gctccgggcc ggaggacgtg
     2401 tgggaggagc cgcgcctgct cgtcatcgct ttgggcgaag agtccggggg cttgcggctg
     2461 tccgtcgcga gcgacgcggt ggtgctctgc cgcgggtgcg gcggcgtgtt cggcgacccg
     2521 ttcgaggcga ttgatgtcga gcgcggcacg ctcgtcatca gacactactc cggctcgcgc
     2581 gaccgctggg gcttcatcga ccgcttccgc ttacaggacg gacgctggac gcacatcggc
     2641 gccaccgaga gacacaccga cacgctcgac tgctcatact tcgaggagcg cgacgccaac
     2701 ctcatcacgg ggcttgtaat cgagagcggc aagatggggc gcagttccta caaccgcacg
     2761 ttctacgagc tgcgggccgc gctcattgaa cgtgcgccgg ccgtggacgg cgcggcctcc
     2821 gccggcgagt ggggcgaccc gcacgtcgtc cgcctcgccg cgaaggagca agtcgccgtc
     2881 ggcacggcgc tgtggcgcgg cgccgacgac gcctcggcgc gcctgtcggc ctcctggcgc
     2941 ggcggcgaac tctacctgcg cgccgaagtc gcggacgact cggtcacgcc cggcgacgcc
     3001 gtccgcctca tctccaaaac ggggcaggtg gtcaggcccg tcgagatgaa gagcgcgccg
     3061 cgcgagggcg gctatgcggt cgaggcgcgc tacacactga agtcgctggg gctggaggaa
     3121 cttgaggcgc agatacgcga gctgagggga tacgcggccg acaaggacat cagcggcgac
     3181 ctgccgctgc gcgtctccgt cgaggtcata gacgaggacg cggggcagaa gactgcttcc
     3241 gtcctctcga caagccgcgg cgggcgcaag tacccggcgc gtgtgcgcct caccagacac
     3301 gcgggcctgc cactgctctc ggacttcgac cgcgagagca gcggcgacgt gctcccgcca
     3361 cccggcttcg gcgtcgcccg gccgtaaatc aaaggatgaa ggatgagggc acgcacagta
     3421 ccgcgagcgg tagcgagcgg gtgtgcgtac tctggcaagg cgtgacctac agccgcaccc
     3481 gctcgctacc gctcgcggta ctgtgtcgcg cttactacgt gggtagtccc ttcgtggcga
     3541 cgcgcttcat cacttcgcag aagcggtcga gttcgttgag ggtcgtgtag acgttgggcg
     3601 tgatgcgcag gccacggaat tcttcgtgga cgatgggcgt ggtgaaaatc ttgtgcgcgc
     3661 ccatcaggtg cgcgccgagc gcgccgggtt cgacgccttc gatctccacg gtcgcgatgg
     3721 cgcacgactg ccgctcgtca aacgaggtgt ggaagcgcac gcgcggcacg tcctttaatt
     3781 gattcatcca gtggcgcgca agaaatcgca gacgcgcctc cttgcgcgcg gggccgaggc
     3841 cgcggtggaa gaggatggct tcgccgatgg cgaggcgggg cgcggccgag tgggtgccga
     3901 tctcctcgaa cttgcgaatg tccgtgtcct gcttgccgtc ggccgccatc agcggccaca
     3961 ccttcgcaat cttgtcccgc ttcacgtaga gcagccccgc gcccttcggc gcgtagagcc
     4021 acttgtgcag ggaagaagcg aagtagtcgc aactgaggcg gtcgcggctg aagtcgaact
     4081 gcgcgaagga gtgcgcgcca tcgacgaccg tctctatccc gcgcgcgcgg gccatctcgc
     4141 agacctcgcg gacgggcgtg atctggcccg tgatgttgat ctggtgcgaa acgagcagga
     4201 ggcgcgtgcg tggcgtgact gcgcgctcga aggcggccgt gatctccgag gggttctttg
     4261 gcgcgagcgg aatcttcacg agcttgaggc ggacgccgtc gcgccgctcg cgctggcgga
     4321 gcgtggtgag catgcgcggg tagtcctgcg tggtcgaaag aatctcgtcg ccgggtttga
     4381 aatcgaagcc gaacagtaaa gcctccaacg actccgacgc gttgcgcgtg atggcgatct
     4441 cctcgcggtc gcagccgaag agttcggcga gacccgtgcg aatcgtctcc gactgcggct
     4501 ccagaatctg ccacatcgtg taggcggtgg cgtcctcctg ctgccagatg taacgcacca
     4561 gcgcctcggt gacgatgcgg ggggagggcg agacgccgcc gttgttgagg ttgacgattc
     4621 cgcgcgtcac ggtgaacgcc tgctggatgc tgaaccagaa gtcctcgtcg cgcgcggcct
     4681 cctccggcgt gaggtgcgcg acgcggcgcg aagcagcctc cacgtctccc agcaacgagg
     4741 ccaccgccgg cgacgcgagc gtggcgaggc cgacgccctg accgacggcg gacaggaagc
     4801 ggcggcggtt gacggaaatt ttgctgcggt tgctttctct catagcgtgc tcctcgcgga
     4861 agttaatcgg catagggttt caacttcctc atgtcctcgg ccgtcgccgg cttcaaactg
     4921 accgccgcgc cgccgcccgg cgccagcttg agcttgagca ccgtgcgatt gtccaccaga
     4981 taactctgaa tcttgtacgc catcgggttg cgctcccagt gcgcgtcgtc ggcgtcggcg
     5041 tagacggtgg cgacgtagcg gcggcccgcg gcgagatggc tcaaggggac gctggcggtg
     5101 cgcgcctcct catccgtgat cgcgccgacg taccactctt cacggccgcg cgcccttcgc
     5161 gcgacgctca ggtagtcgcc cggctcggcc tcaaggatgc gcgtctcgtc ccaatcgacg
     5221 ccaacgtcct tgatgaactg gaaggcgtcc agatgcttct cgtagttctc gggcaggtcg
     5281 gcggccatct ggagcgggct gtagagggtg acgtagaggg cgagctgctt ggcgagcgtg
     5341 gtgtgtactt gttccttctt gcccggctgg tagtagtcca tcttgatctg gaagatgccg
     5401 ggcgtgtagt ccatcggccc gcccatgagg cgcgtgaagg gcaggatcgt ttcgtggtcg
     5461 ggcgggttgc ccgcgctcca cgcgttgaac tcgttgccgc gcgcggcctc gttggcgagc
     5521 cagttcgggt aggtgcggtg cagacccgtg gggcgaacgg attcgtgcgc gttgagcatg
     5581 atcttgtagg cggcggtctt ctcggcgacg cggacaaagt gattaaccat ccactggccg
     5641 tcgtgccact cgccgcgcgg gatgatgcgg ccgacgtagc cggtcttgac ggcgtcgtag
     5701 ccgtgcctct tcataaagcg ataggcgtcg tccatccgcc gctcgtagtt cgtcaccgac
     5761 gaagaggtct cgtggtgcat gatgagcttg acgcctttcg acgccgcgta ggtgtgcagc
     5821 tcctcgacgt tgaagtccgg gtagggcgtg acgaagtcga agacttcctc cttccagttg
     5881 ccgaaccagt cctcccagcc gacgttccag ccctcgacga gcacgcccga gaagccgtgc
     5941 gcggcggcga agtcgatgta gcgccgggtg cgcgccgtgg tcgcgccgtg gcggccgttg
     6001 ggcttgagct tcgaccagtc cgtctccgag agcttgatgt tgccggtgtc ggcgtaattc
     6061 cacgaagact tgttcgggac gtgcatctcc caccagatgc cgacgtactt gatgggctta
     6121 atccaactcg tgtcagcaat cttcgacggc tcgttcagat tcaggatgag ccgtgacgag
     6181 aggatttggc ccgcgcggtc gctgacgacg acggtgcgcc acggcgtccg agctggcgcc
     6241 tgcaagtaag ccttattgcc gacggggtcg ggcgtgagat aggaagtgag gccgaaggtc
     6301 tggcggttga ccgacaaaag catgccggga tagccgacga gcgcggcctc gtggagattg
     6361 atgtagaggc cgtcggccgt cttcatcatc aaaggcgtct gtacgaggtt ggcggcgatg
     6421 aaggtgcgtg tggcgatctc ctgcgaagcc ttgccgcgct cggcgtcgat ctcggagagg
     6481 cgcgtcgtgg agtaggcgta ctcgttcgtg tcgtagtcgc cgggaatcca gaaggtcttg
     6541 tggtcgccgg cgaggttgaa ctcggtgcgc tcgtccgtga cggtgaagta gcgcaagtgt
     6601 tcctgctcgg ggaactcgta gcggaagccg agtccgtcgt cgaagaggcg gaagcgaagg
     6661 atgagcgtcc gctctttcgg gccgggctgg cggagcgtga cggccagctc gcggtagttg
     6721 ttgcggatgc gccgctcctc gccccacacc gtctcccacg tctcgtccgt cacggccgtg
     6781 tctgacttcg ccacggtgaa gcctttggtg agcgcgggct ggtctttcag ctccatgccg
     6841 agccggctct tgcggacgac gctcttgccg gcgaacgaga gttcgtaagc cggctcgccg
     6901 tcggccgtca gcgaaaagac gagcgaaagt tttccgttcg gcgactggac ggtctgcgcg
     6961 gaggcgcaca ggctcaggat gagcaggagg aagacaatta acagtatctt tcgcacggta
     7021 tgattaaccc ccctcgcagt aatcgaaggc cgaagtgttt ctgtaggggc agggcaagtt
     7081 ctcggggtag ggcaaaggca gtagcccgac cgtgagggag ggcgtcacgt cgaggcgccc
     7141 tgcctcacgg tcgggctact gccttgcgcg cgcctccgtt gaaatctcga atcagcactt
     7201 tcttcatcct gcctattctg tccatccatg ttaattgctt ccattgccat gcccctacat
     7261 atttaacgat gccgtcacgg cctcgccgct gcctcgtgga cgacgcggcc gttgaagacg
     7321 gtgatggccg tctcggtctg caaaatctct tccggcttga cggtcaacag atcgcgcgtg
     7381 tggacgacga ggtcggcgag catcccggcg gcgatctgtc ccttctcgcg ctcgtcgaag
     7441 gaggcgtagg ccgagccggt ggtgtagtag cggatggcgt cttcgatgga gaggcgttcc
     7501 tgcggccacc agccgccggc ggggtcggcc ccttcggcgc tctgtcgcgt gacggcggcg
     7561 tagaggccga ggtaagggtt gatcggctcg accggccagt cggtgccgaa ggcgacgggg
     7621 acgccgtgcg ccttcatcgt gtgccacgag taagcgccga gcacgcggta ctcgccgacg
     7681 cgcgcctgcg cccaacgctt gtcggagatg gcgtgggcgg gctgcatcga ggcgacgacg
     7741 ccgaggtcgc ggaagcgcgc gaagtccgtg ggcgcgacga cctgcgcgtg ctcgatgcgg
     7801 tggcgcggtg acaagattcc ggtcgagacc actcgctcgg acgaccccgg cggggtcgtc
     7861 cttatcgtgt actcgaccct tagcgtcccc atctgcgttc cgtttttctc ccgcgattgt
     7921 ctggccgcat caaacccgtc gagggccatg cggttcgcgc ggtcgccgat ggcgtgaagt
     7981 gttatttgaa atccggcggc gtcgcgctcg acgatcatgc gcgtcacctc ttccgccggt
     8041 cggcgcggga ggccgctgtt cgaggggtcg tcggagtaag gcgtgatcat ggcggcggtg
     8101 cgcgagccga gcgtgccgtc catcacgcct ttgagcgcgc cgaggcgcag gacgagcggg
     8161 tcgtccttga atgcggcgaa ctccgcgcgt tgtcgcttca gctccgcgac cgtgtccgag
     8221 aagtcctgcc actcggagac gcgcacggtg agccggccgc gtttgagcag ttcgcgataa
     8281 agccgcgtcg tgcggtagcc ggagttgtcc tgaatcgagg tgatgccgta gcggcgggcc
     8341 tcggcgagcg cgcgctcaag cgcctccatg ttctgctcgt ccgtgggcgc ggggatgacg
     8401 cgcccgacga gaccctgcgc cgtctccttc acaatccccg tcggctcgcc cgccgagtcg
     8461 tgcaggattt cgccgccctc gggcgcgcgc gtctcgcgtg tgagtttggc ctcccggagc
     8521 gcgaccgagt tggcccaact gacgtgcccg tccacgcgcg tcaggaagac ggggttggcc
     8581 ggcgcgacct cgtccaaatc tttcttcgtc ggccactcgc cgccccacaa cgtgtgatcc
     8641 cagccgcgtc ccagaatcca ctcgccggga cgcgccgcgc gcacgcgctc ggccacgcgt
     8701 cgcttcgcct cggcaagcgt cggagcgccg ttgagttcga ctctgaggag ggccagtccg
     8761 ccgttgagaa aatggatgtg ggcgtcgtta aagcccggcg tgacgagccg gccgcg
//
LOCUS       DASVES010000105         4896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_83675, whole genome shotgun sequence.
ACCESSION   DASVES010000105 DASVES010000000
VERSION     DASVES010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83675"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VF240_03775"
     CDS             <1..1164
                     /locus_tag="VF240_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174385.1"
                     /translation="KDVPLEPVTAELRPAGRIAGDGPFFLLKDTGQESLLAARQRLAG
                     YRIEIAERAFKVGAAEYRAGSWVIPAQKDLRAALERVSAELGLDFEAAPLAPEVARHE
                     SRLPRLAVWHTWDDTQAVGWIRYTLDQQKIAYDYISDDQVRAGNLRRRYDVILYGHTF
                     LGLQGQIHGIDKKYGPMPFTKTNAYPSHGVPDSSEDITGGIGWGGMAHLEKFLADGGT
                     LITLGGGSQLALDGGLVREVRRGPGGVFTPGVEVRAKFSRPDHPLAYGYPEVISAFRS
                     NFPVYNVGPAERHLVVLQWGTKPPSEEREEPPAGADKDKPKEKDAGPMLISGGMRGED
                     TLEGRPAILDLPAGRGRVLAYNFNPMHRDLNRSDYRLLWNALLNWSALPPPRR"
     gene            1179..2147
                     /locus_tag="VF240_03780"
     CDS             1179..2147
                     /locus_tag="VF240_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX7174386.1"
                     /translation="MSEVPIQTDARAGEVAADRPPSDVRDDGDDGRAEAAPTGGARVV
                     FREAGHVTLVALGVLSAGLGLKGFLLSSRFIDGGVTGVSMLLADVTGWSLSILLLVIN
                     LPFIALGYRQIGRAFAFKSALAIAGLSVCLAFVKYPDVTPDKLLTAVFGGFFIGAGIG
                     LAIRGGAVLDGTEVAALLVSKKSSLLRVGDVILMLNVVIFSAAALLLGVESALYSILT
                     YFAASKTIDFLLHGIEEYTAVHIISGRSEEIRRRIIRELGRGVTVYKGRGGLSNAEQD
                     ILLCVVTRLEIGRVKGLARQIDASAFVMVHPLSDAAGGVLKRPALH"
     gene            2249..2689
                     /locus_tag="VF240_03785"
     CDS             2249..2689
                     /locus_tag="VF240_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174387.1"
                     /translation="MKHTPNECYIDVWREAGFKGETRRLHGPASHPHLHFREGDWADD
                     IGSLRLGPHAFVLAFREENYADAMLTLGPNDEVYDMRALKFDNEIESLRLINSLKIFD
                     HLHENVGDTAITTTHAPDAGRAESSAEQSQGQQKTRGRGKNTRR"
     gene            2744..4111
                     /locus_tag="VF240_03790"
     CDS             2744..4111
                     /locus_tag="VF240_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7174388.1"
                     /translation="MKTIKLFALILGLMPIIATAQTKSKPIVFTHVTVIDAVAGAVDA
                     RSDMVVVVADGKVADIGKFGKVKIPKQSQMIDASGKFLIPGLWDMHVHLTKAGEHTLP
                     LFIVNGVTSVRDMGGDYVQVLKWRKEAADKTRLSPRIKTAGALLESAENVARMKREGT
                     VEPVDKYRIGVATPEDARKAVDLMASLGVDFIKFRTVASPETYKAIAEASKAKGLPFV
                     GHQFGSPETMIDAGQKSIEHYIFPPIITNPKHAEIYRKMAESGATIVTTIVVADKSLL
                     VSYDDAKTIVEDRAGTVEFRRKYLSGYLIEDWKEQLEEKKEPSPIDLAKLKEVLIKDF
                     QEMHKAGVRLMPGTDVGVLLIYPGSTLHDELQLLVRYVGLTPMQAIVSATRYPAEFFG
                     MQKTLGTIEKGKIADLVLLDANPLDDIANTKRIAAVVLNGKYLSKTLMERMLAETEAA
                     AGKQR"
     gene            4108..4560
                     /locus_tag="VF240_03795"
     CDS             4108..4560
                     /locus_tag="VF240_03795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX7174389.1"
                     /translation="MSRWTNPEEWERYRSGETCPVCLRGKPTDIIAELDASYLMAGED
                     SPMKGYCFLMLKRHAVELYELSAEEAARFMRDVQRASKAVHEITKAVKLNYEIHGNTV
                     PHLHLHLFPRYVGDPFENQPINPRLIKTPVYAPGEFAEFARRLRAGVE"
BASE COUNT         1014 a         1515 c         1511 g          856 t
ORIGIN      
        1 aaagacgtgc cgcttgagcc ggtcaccgcg gagcttcggc ccgcgggccg gatcgcgggc
       61 gatggcccct tctttcttct caaggacacg gggcaggagt ctctgctcgc ggcccggcag
      121 cgtctcgccg gctaccggat agagatcgcg gagcgagcgt tcaaggtcgg cgccgccgag
      181 taccgcgccg gctcatgggt catccccgcg cagaaagact tgcgcgcggc gctcgaacgc
      241 gtctccgccg aactcggctt agacttcgag gccgcgccct tagcgcccga ggtcgcgcgc
      301 cacgagtcgc gtctgccgcg ccttgccgtc tggcacacgt gggacgacac gcaggccgtc
      361 ggctggatac gctacacgct cgaccagcag aagatcgctt acgactacat cagcgacgac
      421 caggtgcgcg ccggcaacct ccgacgccgc tacgacgtga tcctctacgg ccacaccttc
      481 ctcggccttc agggacagat tcacggcatc gacaaaaagt acgggccgat gcctttcacg
      541 aagacgaacg cctacccgag ccacggcgtg cccgactcct ccgaagacat cacgggcggc
      601 atcggctggg gcggcatggc gcatctggaa aaattcctcg ccgacggcgg cacgctcatc
      661 acgctcggcg gcggctcgca gctcgcgctc gacggcggcc tcgtccgcga agtgagacgc
      721 ggccccggag gggtgtttac gcccggcgtc gaggtgcgcg ccaagttctc ccggcccgac
      781 cacccgctcg cctacggcta cccggaagtc atctcggcct tccgctcgaa cttccccgtc
      841 tacaacgtcg gcccggcgga gcgtcacctc gtcgtcctcc agtggggcac caagccgccc
      901 agcgaagagc gcgaggagcc gcccgccggc gccgacaagg acaagccgaa agaaaaggac
      961 gccggcccca tgctcatctc cggcgggatg cgcggcgagg acacgctcga aggccgcccc
     1021 gccatcctcg atctcccggc cgggcgcggg cgcgtgctgg cctacaactt caacccgatg
     1081 caccgcgacc tcaaccgctc cgattatcgt ctgctctgga acgcgctcct caactggagt
     1141 gcgctgccgc cgccgcgacg ctgaagggat gacctccgat gtcagaagtg ccgatacaga
     1201 cagacgcccg cgcgggagag gttgccgcag accgtccgcc gagcgacgtg cgggacgacg
     1261 gcgacgacgg ccgggccgag gccgcgccaa cgggcggggc gagagtcgtg ttcagggagg
     1321 ccggccacgt cacgctcgtc gcgctgggcg ttctgtcggc ggggctgggg ctgaaaggtt
     1381 ttctgttgtc gagccgcttc atcgacggcg gcgtgaccgg cgtctcgatg ctcctcgcgg
     1441 acgtgacggg gtggtcgctg tcaatcctgc tcctcgtcat caacctgccc ttcatcgcgc
     1501 tcggctaccg gcagatcggc cgggcgttcg ccttcaagag tgcgctggcg attgcgggcc
     1561 tctccgtctg cctcgcgttc gtcaagtacc cggacgtgac gcccgacaag ctgctcacgg
     1621 ccgtcttcgg aggcttcttc atcggcgccg gcatcggcct cgccatccgc ggcggcgcgg
     1681 tgctggacgg cacggaggtg gcggcgctgc tggtgagcaa gaagagcagc ctgctgcgag
     1741 tcggcgacgt gatcctcatg ctcaacgtcg tcatcttctc ggccgccgcg ctgttgctcg
     1801 gcgtcgagtc ggcgctctac tccatcctca cctacttcgc ggcgtcgaag acgatagact
     1861 ttctccttca cggcatcgag gagtacacgg ccgtccacat catttcgggg aggagcgagg
     1921 agatcaggcg ccgaatcatc cgcgagctgg gacgcggcgt gacggtctac aagggccgcg
     1981 gcggactctc caacgcggag caggacatcc tgctctgcgt ggtgacgcgc ctcgagatcg
     2041 ggcgcgtcaa agggctggcg cggcagatcg acgcgtcggc cttcgtcatg gtgcacccgc
     2101 tctccgacgc cgccggcggc gtgctcaagc gaccggccct gcattagcga aagatttcat
     2161 tgactcaaaa cagtaccgcg agcagtagcg agcgggtggc gctgtcgaga gtagacccgc
     2221 tcgctaccgc tcgcggtact gaggtagcat gaagcacacg cccaacgaat gctacatcga
     2281 cgtgtggagg gaggccggct tcaaaggtga gacgcgccgc ctccacggcc cggcctcaca
     2341 cccgcacctg cacttccgcg aaggcgactg ggccgacgac atcggcagcc tccgtctcgg
     2401 cccgcacgct ttcgtcctcg ccttccgcga agagaactac gccgacgcca tgctcaccct
     2461 cggccccaac gacgaggtct acgacatgcg cgccctcaag ttcgacaacg agatcgaatc
     2521 cctccgcctc atcaactctc tcaagatttt cgaccatctc cacgaaaacg tcggcgacac
     2581 cgccatcacc accacgcacg cccccgacgc gggccgcgcg gagtcgagtg ctgaacagtc
     2641 tcaggggcag cagaagacga gggggcgcgg gaagaatacg cgacgttgaa tgcgtcagga
     2701 gttcaaagcg cctgtcgcgc gacaagatgg aaggatgaag tttatgaaaa ccatcaagtt
     2761 gttcgccttg attcttggtt tgatgccgat catcgccacg gctcaaacga aatcaaagcc
     2821 gattgttttc acacacgtca cggtgattga cgccgtggcg ggcgctgtag acgcgcggag
     2881 cgacatggtc gtcgtggttg cggacggaaa agtcgcggac atcggaaagt tcggcaaggt
     2941 aaaaattccg aagcagtcac agatgattga cgcgagcggc aagtttttga ttcccggcct
     3001 gtgggacatg cacgttcacc tgacgaaagc gggcgaacac accttgccgc tttttatcgt
     3061 caacggcgtc acgagcgtcc gcgatatggg cggcgactat gtgcaggttt tgaaatggcg
     3121 aaaagaagcc gcggacaaaa cacgcctttc gccgcgaatc aaaactgccg gcgcactact
     3181 tgaaagcgcc gaaaatgtcg cccggatgaa acgtgaagga accgtcgagc cggttgataa
     3241 atatcgcatc ggcgtggcca cgccggaaga cgcgcgaaaa gcagttgatt tgatggcgag
     3301 tcttggcgtc gattttatca agttccgcac cgtcgcctcg cctgaaactt acaaagccat
     3361 cgcggaagca tcgaaagcga aaggtctgcc gttcgtcggg catcaattcg gctcgcccga
     3421 aacgatgatt gacgccgggc agaagagcat cgaacattat atttttccgc ccatcatcac
     3481 gaacccgaaa cacgccgaga tttatcggaa gatggcggag agcggcgcca caatcgtgac
     3541 cacgatagtc gtcgcggaca aatcgcttct ggtttcctac gacgacgcga aaacgattgt
     3601 tgaagaccgg gcgggcacag tcgagtttcg gcgcaaatac ctttcaggtt atttgattga
     3661 agactggaaa gagcagcttg aagaaaagaa ggagccttcg ccgattgatc tggcgaagct
     3721 caaagaagtc ttgataaaag attttcagga aatgcacaaa gccggcgtgc gtctgatgcc
     3781 cggcactgac gtcggcgtgt tgttgatcta tcccggctcc actctgcacg atgagctgca
     3841 actgttagtg agatacgtcg gcctcacgcc gatgcaggca atcgtcagcg cgacccgcta
     3901 cccggcggag tttttcggaa tgcagaagac gctcggcaca atcgagaaag gaaaaatcgc
     3961 cgacctcgtc ctgctcgatg cgaacccgct tgacgacatc gctaacacaa agcgaatcgc
     4021 cgccgtcgtc ctcaatggaa agtatttatc gaagacgtta atggagagaa tgctggcaga
     4081 gacggaagcg gccgccggca agcaaagatg agcaggtgga ctaatcccga agaatgggaa
     4141 cgatatcgca gcggcgagac gtgccccgtc tgtctgcgag gtaagccgac agacatcatc
     4201 gcggagcttg acgcctccta tctcatggcc ggcgaagact ccccgatgaa aggctactgc
     4261 ttcctcatgc tcaaaagaca cgccgtcgaa ctctacgagc tatcggccga ggaggcggcc
     4321 cgcttcatgc gagacgtgca gcgggcgtcg aaggcggtgc atgaaattac gaaggcggtg
     4381 aagctcaact acgagataca cggaaacacg gtgccgcacc ttcaccttca cctgttcccg
     4441 cgctacgtcg gcgacccttt tgaaaatcag ccgatcaatc cccggctgat aaaaactccg
     4501 gtgtacgcgc cgggcgagtt cgccgagttc gcccgcagac ttcgcgcggg cgtcgagtaa
     4561 aatgccggcg ggcccgcgtt ggcctttcac gctcaaaagg ggtgtcgggc cgaatgattt
     4621 ggtaaaacta ggaggcttgg ttaggtctct cccgatggcc tggcttcagc gttagatttc
     4681 ttccttcaat cggagcgaca atgataattt agagacagcg aaatggaggg acatcttatg
     4741 gcggcgacat ttgatgaaga gaaattgaaa gagttgttga aatcggctct ggtcgaagcc
     4801 cttgaagagc atcgtgagct ggtgcaggat attgtcgaag aggcgttgga agacttcgct
     4861 ctggcgcgcg ctatcgagca ggggcttggg ggcgaa
//
LOCUS       DASVES010000106         9511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_623126, whole genome shotgun sequence.
ACCESSION   DASVES010000106 DASVES010000000
VERSION     DASVES010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623126"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VF240_03800"
     CDS             complement(<1..363)
                     /locus_tag="VF240_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7174390.1"
                     /translation="MSETSSAWPDSRGYWGKYGGRYVPETLMAPLEELTARYLEIRDD
                     EEFRARFESLLRHYAGRPTPLFYAARLTAHAGGARIFLKREDLSHTGSHKINNALGQA
                     LLAILMGKRRVIAETGAGQ"
     gene            complement(689..2752)
                     /locus_tag="VF240_03805"
     CDS             complement(689..2752)
                     /locus_tag="VF240_03805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7174391.1"
                     /translation="MTYPKLRLLLTFSIILQLTTPALASGTQDKSAPPTAWTPELALK
                     VKTIASVRVSPDGRRVAYGVTSAVTTPERSELVTQIHVANTDGSEAFQATFADKSSTN
                     PRWSPDGRWLGFTSNRSGKNNLYVMRMSGGEAEQLTEVKTAVGNFQWSPDGLSIAYTM
                     ADPPSEDEEKGQKGRDDSRWVDENVKMSRLYVVAVQKDAAGKREPRKLTKADYHVGGD
                     FDWSPDGKQIVFSHTKAPKANYWTTGDVSVVDVAGGEVKPLAATRAAEAAPVFSPDGK
                     WVALTVTDDPPRWAQAATIQIMPAAGGAPKPLAVSFDAQPNVAGWSADGRAIYFSESR
                     GTGTRVYSINLGANTITEIDKGVEVVSGIDLNAAGTMFGFTSQTSERAPEAYVSRVDN
                     FAPVQISRANADIPKLPLGKTEVVRWKSTDGKEIEGLLTYPVGYQKGQRVPLILNVHG
                     GPAGVFQQTFIGGRGAYPLAAFAARGYAVLRVNPRGSSGYGVEFRRANIKDWGGGDYN
                     DLMTGVDHVVSMGVADPERLGVMGWSYGGYMTSWMITQTKRFKAASAGAPVTNLMSFT
                     GTADIPDFIPDYFGAQPWDNMEVYRKHSPMFNVKGVTTPTMIQHGEADERVPISQGYE
                     LYNALKTQGVPTRMIVLPRQPHGPNEPKMLLKVMQSNLDWFDKYIMGAGEKSGETERS
                     TGKLN"
     gene            complement(2757..3707)
                     /locus_tag="VF240_03810"
     CDS             complement(2757..3707)
                     /locus_tag="VF240_03810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7174392.1"
                     /translation="MSATARSGEKRLAQPRDQGRVLLITDDAATNACVATLEGAGLAV
                     FGVEGGGAAALVALQRTRPHVVVADVSLKGISAGELARSLARVEDATPVVFIGAAPAD
                     AARRVQAIADGAFDYFQVPAESALLVARLGQLVRIRQTIDRLRAEADLDALTGLANRR
                     RFRKSLDREVERWRRYAVPCALLLLDVDHMKKINDTHGHPAGDLVICAVAAALSQHSR
                     DNDTSARLGGEEFGLLLAGADERNAAAAAERVRRAIAEMPVEGVGQFTVSIGVANCPG
                     HARSERELFAASDGALYRAKQLGRDRVEVADGAADLTKEN"
     gene            complement(3848..4480)
                     /locus_tag="VF240_03815"
     CDS             complement(3848..4480)
                     /locus_tag="VF240_03815"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX7174393.1"
                     /translation="MVKVKICGITNLSDALTAVEVGADALGFNFWAGSRRYLTPAAAR
                     EIVAQMPAGVMCVGVFVNEAAPVDVERAAAEADVGAVQLHGEETPEFCAGVKGFEVIK
                     AVRVGGGFRSEMVVRFATEAVLLDAYSESLSGGTGQTFDWALAKEARVHVKKLYLAGG
                     LTPENVGAAVEQVGPYAVDVCSGVESAPGKKDAGRVRAFVEAARAATYKT"
     gene            complement(4489..5322)
                     /gene="trpC"
                     /locus_tag="VF240_03820"
     CDS             complement(4489..5322)
                     /gene="trpC"
                     /locus_tag="VF240_03820"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX7174394.1"
                     /translation="MATQNFLNAIYERKRRRLDAALAVRSLDDVRAAALALRRGAASN
                     TSARSQPHAFASALGVAGRVNIIAEFKRASPSKGVIRSGVSASEMVKLYEEGGAAAVS
                     VLTEEDYFQGSLRDLTEAMACTSLPVIRKDFIFDEYQVYESAAAGADALLLIAAMLED
                     DALARLRLLTEEVLGMDALVEVHTAEEMERAEASGAKLIGVNNRDLQTFEVTLDTSFT
                     LGPDAPPGCLLVSESGIRSGDDIARLRMAGFHAFLIGETLMRAEQPVEALQELTGRLS
                     G"
     gene            complement(5322..6416)
                     /gene="trpD"
                     /locus_tag="VF240_03825"
     CDS             complement(5322..6416)
                     /gene="trpD"
                     /locus_tag="VF240_03825"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7174395.1"
                     /translation="MTEPTHKETRPGWLSTPARRDDAQGATGLRAFVMRLMRGESLGR
                     EEAAGFLDALLDPAATDAQIGAALVALAVKGETVDELAGMAAAMRARAVRLRTRHSLF
                     IDTAGTGSSASKTFNVSTAAAFVIAGAGLPVAKHGSRAATSRSGSADVLAALGVNVSA
                     PPSVMEGCLDEAGVCFMFAPLYHGATARVAGVRRELGVHTTFNLLGPLTNPAGAPRQL
                     VGVWHPALVEPLARTLHALGAERAWVVHGRDGLDEITIADKTVVAEAGGEGVRVFEVR
                     PTDFGYRAASLERLRGGDPEANARIILDVLEGTRRDEARELVAANAAAAILVGGLADT
                     LATAARMAEKSIDEGAALGALDSLRRVTNA"
     gene            complement(6571..7137)
                     /locus_tag="VF240_03830"
     CDS             complement(6571..7137)
                     /locus_tag="VF240_03830"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7174396.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELGAEMDVRRNDAVTVEEVEADCC
                     PDKILISPGPGTPDDAGVTLPLIERFAGRVPILGVCLGHQAIAQVFGGRVVRASEPVH
                     GKAAEICHDGKTVFAGLEYRFRAGRYHSLVVERETLPDCLEVSATTPDGVIMGLRHRE
                     LKVEGVQFHPESILTTEGKKLLANFLKL"
     gene            complement(7313..8059)
                     /locus_tag="VF240_03835"
     CDS             complement(7313..8059)
                     /locus_tag="VF240_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7174397.1"
                     /translation="MWLSPDTSRRELFLRGHDYNEQHFLAIAAEGDVTAAKLFLMAGM
                     SAEVRNEQGETPLILAARGGHTKVVLALLQGGADVNAQDSTGQTALMRAVRQGASDAA
                     RALLERGADINLRTRSGSTALMLAVQRGNAEMVTALLERGADINAVDGETGSALLWAV
                     YYDRPALARLLLEGGADPNVRNNYGGTALMIAATTGSPEVVRLLLESGADAAIKDKDG
                     KDALRWAAEARRPQAVQLLQQYAGGSRKAG"
     gene            complement(8264..>9511)
                     /locus_tag="VF240_03840"
     CDS             complement(8264..>9511)
                     /locus_tag="VF240_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX7174398.1"
                     /translation="ATDYLREYFRGKTLARRAGLAPMAGGAVGCLAYEAARWFEPVLG
                     DGAREQASAGDDALFMIFRTVLAFDRVRQQVEIASVVYTEDADGSRERLHALYDGAVA
                     ETAEIEKSLAGYSRTIAAAQEAAGRGHSASQEVGAGQSFESSFTKEDFERGVGEIKEL
                     ISAGECYQVVLSRRLRKEVSAPPLQIYRALRATNPSPYMYFLRLAGETVVGASPEMLV
                     RCRGQRLDYRPIAGTRRRGATETEDWLLGEEMRADEKEVAEHVMLVDLGRNDLGRVAD
                     FGSVEVSELMIVERYSHVQHLVTSLRARLREGLDRFDALAACFPAGTVSGAPKVRAMQ
                     IIRELEPEPRGLYAGAVLYADYAGNLDSCIAIRTIVLRDGRAEVQAGAGVVADSVPER
                     EYEETLAKARALLNAIEMAERGL"
BASE COUNT         1566 a         3156 c         3135 g         1654 t
ORIGIN      
        1 ctggcccgcg cccgtctcgg cgatgacgcg ccgcttgccc atgaggatgg cgagcagcgc
       61 ctgaccgagt gcgttgttga tcttgtggga gccggtgtgg gagaggtctt cgcgtttgag
      121 gaaaatgcgt gctccgccgg cgtgtgcggt caggcgggcg gcgtaaaaga gaggggtcgg
      181 gcggccggcg tagtgccgga ggagcgattc aaaccgggcg cgaaattctt cgtcatctct
      241 gatttcgagg tagcgggccg tcaactcttc gagcggggcc atcaaggtct cgggcacgta
      301 tcgcccgccg tatttgcccc agtagccgcg cgagtccggc cacgcgctgg atgtctctga
      361 catgcggcct agcttaccac ccggaacttg aggaaggaaa aagtaataag gcgaaaggta
      421 aaagtgataa tgcctaaacg caggcgacga cgagccgccg gaggcggcgg cggaatgtag
      481 ccacgggcgt cagcccgtgg tgtgcgacga gcaaacgatt gaagccccgg aggggcgaaa
      541 gaaatgcgat ggccgtcgta cgcaccacgc atttctttcg cccctccggg gcttccgttt
      601 ttttacccgc tcgttaccca cggctcgcgc cgtgggctac actcttccgc cgctttcagc
      661 ggcacgtcgt cgcctgcggg gcagcaagct aattcaactt gccggtcgat ctctccgtct
      721 caccgctctt ctcaccggcg cccatgatgt acttgtcgaa ccagtcgagg ttgctctgca
      781 tgaccttgag cagcatcttc ggctcgttcg ggccgtgcgg ctggcgcggg agcacgatca
      841 tgcgcgtggg gacgccctgc gtcttgagcg cgttgtagag ttcgtaccct tgagagatag
      901 gcacgcgttc gtcggcctcg ccgtgctgga tcatggtcgg cgtggtcacg cccttgacgt
      961 tgaacatcgg cgagtgcttg cggtagacct ccatgttgtc ccagggctgc gcgccgaagt
     1021 agtcggggat gaaatcggga atgtcggccg tccccgtgaa gctcatcagg ttggtgacgg
     1081 gcgcgccggc cgaggcagcc ttgaaacgct tcgtctgcgt aatcatccac gaggtcatgt
     1141 agccgccata gctccagccc atgacgccca gtcgttccgg gtcggcgacg cccatcgaga
     1201 cgacatgatc gacgcccgtc atcaaatcat tatagtcgcc gccgccccag tccttgatgt
     1261 tggcgcggcg gaactcgacg ccgtagccgg aggagccgcg cgggttgacg cgaagcaccg
     1321 cgtagccgcg cgcggcgaag gcggcgagcg ggtatgcgcc gcggcccccg atgaaggtct
     1381 gctgaaagac gccggccggg ccgccgtgga cgttgaggat gagcgggacg cgctggccct
     1441 tctgataacc cacggggtac gtgagcagtc cctcgatctc tttaccatcc gtgctcttcc
     1501 aacgcaccac ctccgtcttg cccagcggca gtttggggat gtctgcgttg gcgcggctga
     1561 tctgtacggg ggcgaagtta tcaacgcggc tgacgtaagc ctcgggcgcg cgctccgagg
     1621 tctgcgaggt gaagccgaac atcgtgcccg ccgcgttgag gtcgatgccg ctgacgacct
     1681 ccacgccctt gtcgatctcc gtgatggtgt tggcgccgag gttaatcgag tagacgcgcg
     1741 tgcccgtgcc gcgcgactcg ctgaagtaga tggcacggcc gtcagccgac caaccggcga
     1801 cgttcggttg cgcgtcgaag ctcacggcga ggggcttcgg cgcgccgccc gcggccggca
     1861 tgatctgaat cgtggccgct tgcgcccaac gcggtgggtc gtcggtgacg gtcagggcga
     1921 cccacttgcc gtcgggcgag aagacgggcg cggcctcggc cgcgcgggtc gccgcgagcg
     1981 gcttcacttc cccgcccgcg acatcgacca cggacacgtc gcccgtcgtc cagtagttcg
     2041 ccttcggagc cttcgtgtgc gagaaaacga tctgcttgcc gtcgggcgac cagtcgaagt
     2101 cgccgccgac gtgatagtcg gccttcgtca acttgcgcgg ttcgcgcttg ccggccgcgt
     2161 ccttctggac ggcgaccaca tagaggcggc tcatcttcac gttctcgtcc acccagcgcg
     2221 agtcgtcgcg ccccttctgc cccttctcct cgtcttcgct cggcgggtcg gccatcgtgt
     2281 aggcgatgga gaggccgtcg ggcgaccact ggaagttgcc gacggccgtc ttgacctcgg
     2341 tcaactgctc ggcctcgccg ccgctcatcc gcatgacgta gaggttgttt ttgcccgagc
     2401 ggttggaggt gaaaccgagc cagcggccgt caggagacca gcgcgggttg gtggacgatt
     2461 tgtcggcgaa ggtcgcctga aaggcttcgc tcccgtccgt gttggcgacg tgaatttgtg
     2521 tgacgagttc gctgcgctcg ggagtcgtca cggccgaggt cacgccgtag gcgacgcgcc
     2581 ggccgtcggg cgagacacgc acgctggcga tggtcttcac cttgagcgcc agctcgggcg
     2641 tccacgcggt cggcggcgcg ctcttgtcct gagtgccgga agcaagcgcc ggggtcgtga
     2701 gttgcaggat gatggaaaat gttaacagga gtcgaagttt cgggtatgtc atatcattaa
     2761 ttctccttcg tgagatcggc ggccccgtcg gccacctcga cgcggtcgcg gcccaactgc
     2821 ttggcgcggt agagcgcgcc gtcgctcgcg gcgaagagtt cgcgttcgga gcgagcgtga
     2881 ccggggcagt tggccacgcc gatgctgact gtgaattggc cgacgccctc gaccggcatt
     2941 tccgcgatgg cccggcgcac gcgctcggcg gcggcggccg cgttccgctc gtcggccccg
     3001 gcgaggagca gcccgaactc ctcaccgccg aggcgcgcgc tcgtgtcgtt gtcgcgcgag
     3061 tgttgcgaga gcgcggcggc gacggcgcag atgacaaggt cgcccgccgg gtgcccgtgc
     3121 gtgtcgttga tcttcttcat gtgatccacg tcgagcagca ggagcgcgca cgggacggcg
     3181 tagcggcgcc agcgttccac ttccctgtcg agcgacttgc gaaagcgccg gcggttggcc
     3241 agccccgtca gggcgtcgag gtcggcctcg gcgcgcaggc ggtcaatggt ctgccggatg
     3301 cggacgagtt ggccgagccg cgcgacgagg agcgccgatt cggccgggac ttgaaagtag
     3361 tcgaaggccc catcggcgat ggcctgcacg cggcgagccg cgtccgccgg ggccgccccg
     3421 atgaagacca cgggcgtcgc gtcctcgacg cgcgcgagcg agcgcgccag ctcgcctgcg
     3481 ctgatgccct tgagactcac gtcggcgacg acgacatgtg ggcgcgtgcg ctggagcgcg
     3541 acgagcgcgg ccgccccgcc gccctcgacg ccgaagaccg cgaggcccgc gccttcgagc
     3601 gtcgcgacgc aggcgttggt cgccgcgtcg tccgtaatga gcagcacgcg cccctggtcg
     3661 cggggttgtg cgagcctctt ttcgccgctc cgggccgtcg cgctcattaa tctgtgctcg
     3721 actgaaaacg ctccctgcga gaattaatga gggtgtgact aacctgttgg ggcgaaggct
     3781 gcttgccgga aaagcgtggc tcgcacgctg acgaagcgca aggataactc agggtggtcg
     3841 ccgcgcgtca agttttatac gtggccgcgc gcgccgcctc gacgaaggcg cgcacgcgcc
     3901 ccgcgtcttt cttccccggc gccgactcca ccccgctgca cacgtcaacc gcatacggcc
     3961 ccacctgctc gacggccgcg ccgacgttct ccggcgtcag cccgcccgcg agatagagct
     4021 ttttcacatg aacgcgcgcc tctttcgcca acgcccagtc gaacgtctgc cccgtaccgc
     4081 ccgaaagact ctctgagtat gcgtcgagca gaaccgcctc cgtcgcgaag cgtacaacca
     4141 tctcgctacg gaaaccgccg ccgacgcgca cagccttgat gacttcaaag cctttgactc
     4201 cggcgcagaa ctcgggcgtc tcctcgccgt gcaactgcac tgcgccgacg tccgcctcgg
     4261 cagccgcgcg ctcaacgtca acaggcgcgg cctcattcac gaacaccccc acgcacatga
     4321 cgccggccgg catttgcgcg acgatctccc gcgccgccgc cggcgtgaga tagcgacgac
     4381 ttcccgccca gaagttgaag ccgagcgcgt ccgcgccgac ctccacggct gttagtgcgt
     4441 ccgacagatt cgtgatgccg cagattttta ctttgaccac cgtcaccctc aacccgagag
     4501 tctcccggtc agttcctgca acgcctcgac gggctgctcg gcgcgcatca gcgtctcgcc
     4561 gatgaggaag gcgtggaagc cggccatgcg tagccgcgcg atgtcgtcgc cgctgcggat
     4621 gccgctctcg ctgacgagca ggcaaccggg cggcgcatca ggcccgagag tgaaagaggt
     4681 gtcgagcgtc acttcaaagg tttgcaggtc tcgattgttg acgccgatga gtttcgcgcc
     4741 ggaagcttcg gcgcgctcca tctcctccgc cgtgtgaact tcgacaaggg cgtccatgcc
     4801 gagcacttcc tccgtcagca gtcggagtct ggcgagcgca tcgtcttcga gcatcgccgc
     4861 gatgagcagg agggcgtcgg ccccggcggc ggccgactcg tagacctgat actcgtcgaa
     4921 gatgaaatcc ttgcggatga ccggaagact cgtacaggcc atcgcctccg tcaaatcccg
     4981 gagcgaccct tgaaagtaat cctcctctgt cagaaccgag acggccgccg ccccgccttc
     5041 ctcatagagt ttgaccatct ccgacgccga gacacctgag cggatcacgc ccttcgaagg
     5101 cgaggcgcgt ttgaactccg caatgatgtt cacccgaccc gcaacaccga gcgcactggc
     5161 gaaggcgtgc ggctgcgaac gcgcggaggt gttcgacgcc gcgccgcgcc tgagcgcaag
     5221 ggccgcggcg cgaacatcat cgagtgagcg cacggcgagc gcggcgtcga gtcgccgccg
     5281 cttccgttcg tagattgcgt tcaggaaatt ttgtgtggcc atcaagcgtt cgtcacgcgt
     5341 cggagactat cgagcgcgcc gagcgccgcc ccctcgtcaa tacttttttc ggccatgcgc
     5401 gccgccgttg cgagagtgtc ggcgaggccg cccaccagaa tcgccgccgc cgcgttggcc
     5461 gcgacaagct cacgcgcctc gtcgcgcctt gtgccttcca acacgtcaag gataatgcgc
     5521 gcgttcgctt cggggtcgcc gccgcgcagg cgttcgagcg aggccgcgcg gtagccgaag
     5581 tccgtggggc gaacttcaaa gacgcggacg ccctcgccgc cggcttcggc gacgacggtc
     5641 ttgtcggcaa ttgtaatttc gtccagtccg tcgcggccgt gaacgaccca cgcgcgctcg
     5701 gcgccgagcg cgtggagggt gcgcgccagc ggctcgacga gggcgggatg ccagacgccg
     5761 acgagctggc gcggcgcgcc ggccggattg gtcagcgggc cgagcaggtt aaatgtggtg
     5821 tgtacgccaa gctctcgccg cacgccggcg acgcgcgccg tcgccccgtg atagagcggc
     5881 gcgaacatga agcagacgcc cgcctcgtcc agacaccctt ccatcacact gggcggcgcg
     5941 gagacattga cgccgagcgc cgcgagcacg tcggccgagc cggaacgact cgtcgccgcg
     6001 cggctgccgt gcttggcgac gggcaggccc gccccggcga tgacgaaggc cgcggccgtc
     6061 gagacgttga aagtcttcga cgcgctcgaa cccgtccccg ccgtatcgat gaagagggag
     6121 tggcgcgtgc ggagacggac ggcgcgggcg cgcatcgccg ccgccatgcc ggccagctcg
     6181 tcaaccgtct cacccttgac ggcgagggcg acgagcgccg cgccgatctg cgcgtcagtc
     6241 gccgccgggt cgaggagcgc gtcgagaaac cccgcggcct cctcgcggcc gagactttca
     6301 ccacgcatca ggcgcatgac gaaggcgcgc aggcccgttg caccttgcgc gtcgtcgcgc
     6361 cgcgccggcg tcgagagcca tccggggcga gtctctttat gtgtcggctc ggtcatgggg
     6421 aagcgatcag ctctcagcaa acgcgggtca gtaccgcgag cggaagcctc gcgggtctgg
     6481 atgcgcgagg cgggcctctc accgcaggcg cacccgctcg ctaccgctcg cggtactgtt
     6541 tgtggtgatg attatggcgc gactgctctt tcacaatttc agaaagttcg cgagcaattt
     6601 cttgccctcc gtcgtgagaa tcgactcggg atgaaattgc acgccctcga ccttcaattc
     6661 gcggtgacgc aggcccatga tgacgccgtc gggcgtggtc gcggagacct ccaaacaatc
     6721 ggggagcgtc tcgcgctcga cgacgagcga gtggtagcgt ccggcgcgga aacgatattc
     6781 gagtccggcg aagacggtct tcccgtcgtg acagatttcc gcggccttgc cgtggacagg
     6841 ctcgcttgct cgcaccacgc gcccgccgaa gacctgcgcg atggcctgat ggccgaggca
     6901 cacgccgagg atggggacgc ggcccgcgaa gcgttcgatg agcgggagcg taacgcccgc
     6961 gtcgtccggc gtgcccggcc ccggcgagat gagaatcttg tcggggcagc agtcggcctc
     7021 cacctcctcg accgtcaccg cgtcgttgcg gcgcacgtcc atctccgccc ccagctcgcc
     7081 gagatactgg acgaggttgt acgtgaagga atcgtaattg tcgatgacga gtatcatttc
     7141 aagagtgata agtgatgagt gataagtgat gagaaatacc ggcaatcgta aatcgtgaat
     7201 cgtaaatcgt gaaagacagt tcctccgatt cacgatttac gatttacgat tcacggcttt
     7261 attacttatc acttatcact catcacttat cacggacgca gtcgcaagtc tgtcagccag
     7321 ccttgcgact gccgcccgcg tactgttgta aaagttggac ggcctgcggg cggcgcgcct
     7381 ccgcggccca acgaagcgcg tccttgccat ccttgtcctt gatggcggcg tcggcgccgc
     7441 tttcgagcag gaggcggacg acctcggggc tgcccgtggt cgccgcgatc atcagggcgg
     7501 tgccgccgta gttgttccgc acgttgggat cagccccgcc ttcgagcagc agacgcgcga
     7561 gcgcggggcg gtcgtagtag acggcccaga gcaaggccga gccggtttcg ccgtccacgg
     7621 cgttgatgtc ggcgccgcgt tcgagcaggg ccgtgaccat ctcggcgttg ccgcgttgca
     7681 cggcgagcat cagcgccgtg gagccgctgc gcgttcgcag gttgatgtca gcgccgcgtt
     7741 cgagcagagc gcgcgcggcg tcggaggcgc cctgtcgcac ggcacgcatc aatgcggtct
     7801 gtcccgtcga gtcctgcgcg ttcacatcgg ccccgccctg aagcagcgcg agcacgacct
     7861 tcgtgtgtcc gccgcgcgcc gccaggatga gcggcgtttc gccctgctcg tttctcacct
     7921 cggcgctcat ccccgccatg aggaaaagtt tcgcggcggt cacgtcgccc tcggcggcga
     7981 tggcgagaaa atgctgttcg ttgtagtcgt gaccacgcag gaacagctcg cgccgcgacg
     8041 tgtcgggcga caaccacacg tcgcctctcc tctgaacaca cgcaccggcc gtggcgatga
     8101 gcaccaacgt cacgacaaga gaaagaagtc ggcagtctga attcattaca atcaaatgga
     8161 caggattaac aggacggaca ggattacgaa agaggtgatt tcagttttga gatttgagat
     8221 tttcaatcat ctcatcctgt tagtcctgta actcctgtct gaactaaagc ccgcgctcgg
     8281 ccatctcgat ggcgttcagg agcgcgcgcg ccttcgcaag cgtctcctca tattcgcgct
     8341 ccgggacgga atccgcaacc acgcccgctc cggcctgcac ctccgcgcgc ccgtcgcgca
     8401 ggacgatggt gcggatggcg atgcacgagt cgaggttgcc ggcgtagtcg gcgtagagca
     8461 cggccccggc gtagaggccg cggggttccg gttcgagttc gcggatgatc tgcatggcgc
     8521 gcaccttcgg cgcgccggag acggtgccgg cggggaagca cgcggcgagc gcgtcgaagc
     8581 ggtcgaggcc ctctctcaga cgcgcgcgca gggaggtcac aaggtgctga acgtgcgagt
     8641 agcgttcgac gatcatcaac tccgacactt cgaccgagcc gaagtcggcc acgcggccca
     8701 gatcgttgcg gccgagatcg acgagcatga cgtgctcggc cacctccttc tcgtcggcgc
     8761 gcatctcctc gccgagcagc cagtcttcgg tctcggtggc gccgcgtcgg cgcgtgcccg
     8821 cgatggggcg atagtcgagc cgctggccgc ggcagcggac gagcatttcg ggcgaagccc
     8881 cgacgaccgt ctcgcccgcg agccgcagga aatacatgta aggcgaaggg ttcgtcgccc
     8941 gtagcgcccg gtaaatctgg agcggcgggg ccgaaacttc tttccgcagg cgacgcgaga
     9001 ggacgacctg ataacattcg cccgcgctga tcaactcttt aatctcaccg acgccgcgct
     9061 cgaaatcttc cttcgtgaag ctcgactcga aagactgccc cgcgccaacc tcttgcgaag
     9121 cactgtggcc gcgcccggcg gcttcctgcg ccgccgctat cgtccgcgag tagcccgcga
     9181 gactcttttc aatctccgcc gtctcggcca ccgccccgtc gtatagtgca tgcagccgct
     9241 cgcggctccc gtccgcgtcc tccgtataga ccaccgacgc gatctccacc tgctgtcgca
     9301 cgcggtcgaa cgcgagcacc gtgcggaaga tcataaacaa cgcgtcgtcg cccgcggacg
     9361 cttgctctcg agcgccgtcc ccaagaaccg gctcgaacca gcgggccgct tcataagcga
     9421 gacagccgac ggcgccgccg gccatcggcg cgagacccgc gcgccgcgcg agcgtcttac
     9481 cccggaagta ttcgcgcagg tagtcggtcg c
//
LOCUS       DASVES010000107        13253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_37416, whole genome shotgun sequence.
ACCESSION   DASVES010000107 DASVES010000000
VERSION     DASVES010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13253
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37416"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VF240_03845"
     CDS             complement(<1..941)
                     /locus_tag="VF240_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7174399.1"
                     /translation="MLRTAVIGVGHLGRHHARVHAALEAEGVSSFAGVCDIDEETARR
                     VAREHETEPATDWRALAERVDAVSLAVPTEAHCEIACALLSSGVHVLVEKPISRTLEE
                     ADRMIAAAESGGAVLQVGHLERFNPALVALRPHVRRPLYFEVHRVGQFTARSLDIDVV
                     LDLMIHDLDIVQWLVGCEVEATEVRAVGIPVLTNRVDAANARIEFASGAVANLTASRV
                     GAEKIRKMRFFQPHDYVAVDYATRHASVSNLAPPSGAGAWPGVNTRVLEVNEVEPLRA
                     EIESFLDSARTGGAPAVTGADGRRALSLATRVLERIH"
     gene            1286..2923
                     /locus_tag="VF240_03850"
     CDS             1286..2923
                     /locus_tag="VF240_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7174400.1"
                     /translation="MQRSTLAKRLWLLLSAAVCLTYLYGLGGYPLVGADEPRYAQVAR
                     EMYERGDLLTPTLGGLHWFEKPPLLYWMMMAGYRVLGVSELAARLGPALSGLLCVLSL
                     GLLALKVEEKLGEAGRWLGLCVAGVLASSTGVLIFSRGASFDIVLTACVTAALSFFLA
                     AELEEDGRRRRFFLAGFYAAVGLATLAKGLVGVLIPAGVVGGYFLLCRRWPRLNRLGV
                     WWGAALALAVAAIWYAPAVARHGWEFIDEFFVQHHFARYVSDKYHHPEPFFYYFYILP
                     LLALPWTVFAARELWESRRLSAVVDSTERKFRAFAAAWLLFPVVFFSFSGSKLPGYVL
                     PALPGAALLAGACLSRYVRGGAGLSLMRATGVGALCLCVAGIVYARQSGSISMKATLM
                     IALPSFIAGVVAVLFTRRRALCVGSLVACAFVTVVLIVSMAAGRFAAVESVHGLLSKA
                     EAEGLGGLPILQLHDIERTSEFYGAGRLVYSAEGQPLKLEGPYEVVREAGERGGRALV
                     IVPPVYLYQLENLRGASTRLVAENGVHALVYVEVGAR"
     gene            complement(2951..3916)
                     /locus_tag="VF240_03855"
     CDS             complement(2951..3916)
                     /locus_tag="VF240_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174401.1"
                     /translation="MPPRPRPGKRIFKALLPVVLLPLLAAVVFALWVVYSASHPPRHA
                     YLMTPEGFGLISERGLQVKEEKWRNRDGTEARGWLLRGGEGAPAVVMLHRYGADRSWA
                     LNLGVMLNEASNVTVLWPDLRGHGENPPVSSTTFGARERDDLLDAFEFLRGLQSPQGR
                     PQVGDRLGLYGVELGAYAALLAARAAPQVKALVLDSTPASADELLSGVVSERVGIDNG
                     LIRLLARGGARLYFLGRYENTSSCIAAETLGEPRVLLLSGNDATHLRTSSEILSKCFP
                     NPSNVELRVGLPLTGFTQASSPAQQGETYARLVIDFFGRSLQPAP"
     gene            complement(4107..4724)
                     /locus_tag="VF240_03860"
     CDS             complement(4107..4724)
                     /locus_tag="VF240_03860"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX7174402.1"
                     /translation="MLDIEGFILAGGASSRMGADKAQLRLGGQTLVETVAGALSEITL
                     TVRVVSSRHGGELCGLTVVPDFYDDCGALGGLQSALTHAEAAWVAVVSCDLPFVTGGL
                     LSRLTTFVKEGVEAVVPVQEDGRVQPLCALYERRQCLEIVERLLREGERRPRVLVAQA
                     RTRLADFALLADLPASHLFFLNLNSPDDYAQAVAALDTSACPARK"
     gene            5025..6899
                     /locus_tag="VF240_03865"
     CDS             5025..6899
                     /locus_tag="VF240_03865"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEX7174403.1"
                     /translation="MIRRLAALFAVTLALCGAAVAQQTVVTPKTGDAAYFPLQEVKAG
                     QKGVAYTVFSGSVPEEFKVEVLGVLPGYPQPRQSIIIARISGPLAERTGVFAGMSGSP
                     VYIDGKLVGAIAYSFPFSKEPIAGITPIQQMIDIFEGGAVQSGERDQLGFPREPRAVS
                     FAQLGGSDFGVALPKSGMTSATFLAPVAAGSPLANLLGQQLAPIATPVVFGGISADTL
                     ARFAPQLQATGLLPVSGVGGASPITPLARFDSKTLLGGTSVSVQLVRGDYSIAAAGTV
                     THRDGERIYAFGHPFLGLGSADMPMSESSVVTVIPNTLNSFKLSVPGQMVGAISQDRA
                     TGIYGQLGRNPKMIPVKINLHTSRGRVETYNYEVASDEFLTPILLNITVYNTISSSER
                     SLGDSTVSVRGQINVKGQDAIKLERRFSAAGAGIAAAGSVAGPVAALMSSGFDGVDLQ
                     SISLDIASADVRRAATLDRIALDRAEVARGETVEVQAYIRTESGKQFVQRIPVQIPAD
                     VPTGPLMLFVGDGGSIQQASAMQSFVPKDLGQLVSAINRLKKNDRLYVRMLRVTHGAV
                     IGTDEMPSLPPSFVATLNSDRSSGGFTPTALSPLGEQELPAAEYVISGQQMISINVVR
                     "
     gene            complement(7081..7671)
                     /locus_tag="VF240_03870"
     CDS             complement(7081..7671)
                     /locus_tag="VF240_03870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEX7174404.1"
                     /translation="METNAARFDFGTDVGVVAGLRAYFEIVMRWNPRLHLVAPCTPAE
                     FAMRHVLESLIAAPHLPAGARVVDVGSGAGLPVIPCLIARPDLRALLVESSRKKAVFL
                     REALVAVSRGDAAEVLAERFQSAPPPAADALTCRALERFTEVIPELLSWSSRVKRLLL
                     FGGHLLGAELEKAGLTFSSVLIPESERRFLFVVDKK"
     gene            7747..9231
                     /locus_tag="VF240_03875"
     CDS             7747..9231
                     /locus_tag="VF240_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7174405.1"
                     /translation="MDSTKRFTAERLGSEHARLGREAVRVVSEYAGSLDRLPVCSRAT
                     PAELEALFDEPLPLDSTPAAEIFAKFERDVLPHAMNIPSPRYYGLFNPTPLPIAVWAD
                     TLASAINQNGAAWRNSPSASVIEARVLRWMCELFGLGAESFGTLTSGGSEANLIGLKC
                     ARDRVAIKARDGGLISAGVTGNLIVYASEQSHYSLVKSADILGLGRQNLRKIDTDERF
                     HIRVDQLRAEIEKDLREGNTPCCVAGAAGATSTGVVDPLDELADVAAEHGLWFHVDAA
                     YGGGLAFSEKHRVRLRGIERADSVTVDPHKWMFMPFACGALLVRGGGRVLRDSFDITP
                     EYLSEDRGGADVEYDFFRYGQLGTRRFNALKIWMALRHMGVSGYASVVERQIDLARHL
                     AARLDALADFECVGEVETALCCARFVPAALRARGGEEIDELQKALQQRIERSGEAWFA
                     TTVLHGRRALRINVNSFLTERRHIDDLVELLRREGASLLAEWKS"
     gene            9247..11184
                     /locus_tag="VF240_03880"
     CDS             9247..11184
                     /locus_tag="VF240_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX7174406.1"
                     /translation="MKTSKLSRVGALLLLSLCAVVLVASPGVRAQETPPPSRAEEGER
                     SVAAIKSRAEFDRVARVYTDRPYALPHVLFVIDRKDKNRIYYVNSKRYRFHKDFVNGT
                     YLSLERGQEFENNYLKPNRRFILGTLAYQAPVRAWTFEFWEGDLIAAELVKLTAELIR
                     QTFYEPVKFKPNSLRQEEASVQVEGLERVLQSDISRGQEYEALNVAKGIGRIHVVEKL
                     DEHVEIGFNEIIVLNEVPLSLPPVAGVIISKPSTPLSHINLLAKGWGVPNAYIKNAHE
                     LLKGYDGWWVNFETFPDKYTIKRADIGDIKKYQDALAAEHVLMTPRFDLAAKSLLDLR
                     RQRARMAPAYGAKSANLGEVMSARLRGVTVPNGFTVPIFYYEQFIKENKLDDAILEMM
                     DDQKFVHDPAYRRERLKEMRAQIQAGKVNEQLRAGVLRRARAEYAGKGLFVRSSSNVE
                     DLPNFSGAGLYDTVPNVLEEEKLIEAIKTVWASVWNFSAYEARERAHVDHTKAFMAVL
                     IQEGINADSAGVMITTDPYDNDNRDAIYISAKRGLGIKVVEGKRVAEQVIYRPRSDSV
                     QVLTRSDEESLLTFDERGGVREVPISGERAVLTDAMVRRLAVAARRVRGLFGGREQDI
                     EWVYRGGLLYIVQSRPFVTGG"
     gene            11191..11703
                     /locus_tag="VF240_03885"
     CDS             11191..11703
                     /locus_tag="VF240_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7174407.1"
                     /translation="MILKTKTRALILSCLAATLTGCNDAPEGTNRAANSAATSSSPAA
                     ERPVEGANLDAEIQRLEKLVERNPGEDSVTRPLAAAYVRRGNNLRASNDLRGALKDYR
                     SAIKYDEDNEEAQKNIAEIVPQVEGEKTGEYGEPAPLPITPNVTTDTDDNAGPSPTAQ
                     PSPRKNVNRE"
     gene            11958..12740
                     /locus_tag="VF240_03890"
     CDS             11958..12740
                     /locus_tag="VF240_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7174408.1"
                     /translation="MNDDVFKDDILKGRVALVTGGGTGITGGVARALAEAGARLALVS
                     RKLEHLEPAAALIREAGGEAVAVAADVRDYEQVERAVAATVEAFGHLDIVVNGAAGNF
                     LCRAEELSSNGFGTVVDIDLKGTFNVCRAAFDELKKNRGQILNISATLHYLGTPLQLH
                     VSAAKAGVDALTRNLAVEWGAYGIRSNAVAPGPIEDTEGMRRLVPPEVRERLLRRIPL
                     GRFGRVRDVERCAVFLCSDAASFINGAVIAVDGGNWLHNKLG"
     gene            12822..>13253
                     /locus_tag="VF240_03895"
     CDS             12822..>13253
                     /locus_tag="VF240_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174409.1"
                     /translation="MIDLQPSTFEAFEREVERGNVVPVVRTVLADLQTPVGAYMLLAA
                     DAPYSFLLESVEGGERIARYSFLGFNPEMVVRGRGADTFVERAGVEVETHAGVRATDY
                     LREYFRGKTLARRAGLAPMAGGAVGCLAYEAARWFEPVLGDG"
BASE COUNT         2291 a         4294 c         4425 g         2243 t
ORIGIN      
        1 tcgtggatgc gttcgagcac gcgtgtggcc agagagagcg cgcgccgtcc gtcggcgccg
       61 gtgacggcgg gcgctccgcc cgtccgcgcg gagtcgagaa aagattcaat ttcggcgcgc
      121 agaggctcca cttcgttgac ctccagaacg cgcgtgttga cgccgggcca cgcgcccgcg
      181 ccgctcgggg gcgcgaggtt gctgacggag gcgtgacgcg tcgcgtaatc gacggcgacg
      241 tagtcgtgcg gctggaagaa gcgcatcttg cgaatcttct cggcgccgac gcgcgaggcc
      301 gtgaggttgg cgaccgcgcc cgaggcgaac tcgatgcgcg cgttggccgc gtccacgcgg
      361 ttggtcagca cggggatgcc gacggccctg acctcggtcg cctcaacctc acagccgacg
      421 agccattgca cgatgtcgag gtcgtgaatc atcaggtcga gcaccacgtc gatgtcgagc
      481 gagcgggcgg tgaactggcc gacgcggtgc acctcgaagt agagcgggcg gcggacgtgc
      541 gggcgcaggg cgacgagcgc cgggttaaag cgttcgaggt gtccgacctg taacacggcc
      601 ccgccgcttt cggccgcggc gatcatccgg tcggcctctt cgagcgtgcg cgagatgggc
      661 ttctcgacga ggacgtggac gcccgacgag agcagcgcgc aggcgatctc gcaatgcgcc
      721 tcggtgggga cggcgaggct caccgcgtca acgcgctcgg caagagcgcg ccagtcggtc
      781 gccggctcgg tctcgtgctc gcgggcgacg cggcgcgcgg tctcctcatc gatgtcgcag
      841 acgccggcga agctcgaaac accctcggcc tcaagcgccg cgtggacgcg cgcgtggtgc
      901 ctaccaagat gtccgacgcc gatgacggcc gttcgtagca tagaagcgat gagctgttag
      961 ctgttagctt gtaaatccgc cgccgggaac gaaatttcag cggaggaatg ccgactgtgt
     1021 ggccgggcct ttgattgaaa agagaacttg aacgatactc gccagcggca atcaagcaga
     1081 actgttagaa gtcggcaagc acagtaccgc gagcggtagc gagcgggtgt ggatgtgcca
     1141 tgcggaactc ccgtcgcagg tagacccgct cgctaccgct cgcggtactg ttcaatgagc
     1201 cgctgaaaac tgagctgtaa aaaaactgaa agctgacagc taatagctaa cagctaatgc
     1261 ctgacagctt tccccgtgaa tatacttgca acgttcgacg cttgccaagc ggctatggct
     1321 cctgctctcg gccgccgtgt gtctcacata tctctacgga ctcggcggct atccgctcgt
     1381 gggcgcggac gagccgcgct acgctcaggt ggcgcgggag atgtacgagc gcggcgacct
     1441 cctgacgccg acgcttggcg gtctccactg gttcgagaag ccgccgctgc tctactggat
     1501 gatgatggcg ggctaccgcg tgctgggcgt ctcggaattg gcggcgcgtc tcggcccggc
     1561 gctctcggga ttgctgtgtg tcctctcgtt ggggctgctg gcgctgaagg tcgaagaaaa
     1621 gttgggggag gcggggcgtt ggctggggct gtgtgtggcc ggcgtgcttg cgtcgagcac
     1681 aggcgtgttg atcttctcgc gcggggcgag tttcgacatc gtcctgacgg cgtgcgtcac
     1741 ggccgcgctc tcttttttcc tcgcggccga acttgaagaa gacgggcggc ggcgccgttt
     1801 ttttctcgcc ggcttttatg cggccgtcgg cctcgcgaca ctcgctaaag gactcgtcgg
     1861 agtgttgatt ccggccggtg tcgtcggggg ctattttctt ttatgccgga ggtggccgcg
     1921 tctgaatcga ctcggcgtgt ggtggggcgc ggcgctggcg ttggccgtcg ccgccatctg
     1981 gtacgcgccg gccgtcgccc gccacggatg ggagttcatc gacgagttct tcgtccaaca
     2041 ccacttcgcg cgctacgtct cggataagta tcaccaccct gagccgttct tctactactt
     2101 ctacatcctg ccgctgctcg cgctgccgtg gacggttttc gcggcccgtg agctttggga
     2161 gtcgaggagg ctgagcgcgg tcgtggattc gacggagaga aagtttcggg cctttgccgc
     2221 cgcgtggctc ctgttcccgg tcgtgttctt ttccttctcc ggctcgaagc tccccggcta
     2281 cgtgttgccg gcgctgccgg gcgcggcgct gctcgcgggc gcgtgccttt cgcgctacgt
     2341 gcgtggcggc gcgggtctct ctttgatgcg cgctacgggg gtcggcgcgt tatgcctgtg
     2401 tgtcgccggc atcgtctacg cgcggcagag cggttcgatt tcgatgaagg cgacgctgat
     2461 gatcgcgttg ccgtctttca tcgccggcgt ggttgccgtg ctcttcacga gaaggcgcgc
     2521 gctgtgtgtc gggtcgctcg tcgcgtgtgc gttcgtgacg gtcgtcctga tcgtgtcgat
     2581 ggccgcgggc aggttcgcgg cggttgaatc cgtccacggc ctcttgagca aggccgaggc
     2641 ggaagggctt ggaggacttc cgattcttca gcttcacgac atcgagcgca cgtccgagtt
     2701 ctacggcgcg gggcgtttgg tctactcggc cgaagggcag ccgctcaagc tcgaaggccc
     2761 atacgaggtg gtgcgtgagg ctggcgagag gggcgggcgg gcgctcgtca tcgtcccgcc
     2821 tgtttatttg tatcaactgg agaacctgcg tggcgcttcg acgcgcctcg tcgcggagaa
     2881 cggcgtgcat gcgctggtgt atgtggaagt cggcgcgcgt tgattggatg aagtcgatgc
     2941 tggataaacg tcagggggcg ggctggagcg agcggccgaa gaagtcgatg acgaggcggg
     3001 cgtaagtttc tccctgttga gcgggtgaag aggcttgtgt gaagccggtg agcgggaggc
     3061 cgacgcgcag ctcgacgttg gacgggttgg ggaagcactt cgagagaatt tcgctcgacg
     3121 tgcggaggtg tgtggcgtcg ttgccggaga gcaggaggac gcgcggctca cccagagtct
     3181 cggcggcgat gcaactggaa gtgttttcgt agcggccgag gaagtagaga cgcgcgccgc
     3241 cgcgcgcgag gagccggatg aggccgttgt ctatcccgac gcgctcagag acgacgccgc
     3301 tgaggagttc gtcggccgag gcgggcgtcg agtcgagcac gagcgccttg acctgcggcg
     3361 cggcgcgcgc ggcgagaagg gcggcgtaag cgccgagttc gacgccgtag aggccgagac
     3421 ggtcgccgac ctgcgggcgc ccctgcggcg actggagacc gcggaggaat tcgaaggcgt
     3481 cgaggaggtc gtcgcgttcg cgcgcgccga aggtggtgga cgagacgggc gggttctcgc
     3541 cgtgcccgcg caggtcgggc cagagcacgg tgacgttcga cgcctcgttg agcatgacgc
     3601 cgaggttgag cgcccaggag cggtcggccc catagcgatg gagcatgacg acggccggcg
     3661 cgccctcgcc gccgcgcagg agccagccgc gcgcctccgt cccgtctcga ttccgccact
     3721 tctcctcttt gacctgtaga ccgcgctcgc tgatgaggcc aaacccctcg ggcgtcatca
     3781 ggtaggcgtg gcgtggcggg tgcgacgcgc tgtagacgac ccacagcgcg aagaccacgg
     3841 cggccagcag cggcaggagc accacgggca gcagagcctt gaagattctt tttccgggtc
     3901 tgggtcgagg aggcatgcgg cgtaatatta aactaattaa caggcggaag agaaaaataa
     3961 aacgcgccgg tagtgtcctg atttcaacac tccgcgcccc ctcggcgtcc tccgcgcctc
     4021 cgcggtgagt caaccccggc gctcattcac cgccgagccg caaaggacgc cgagggggcg
     4081 cggagcaatt cattcaactc aggacactac ttacgcgccg ggcacgccga cgtgtcgagt
     4141 gcggcgacag cctgtgcgta atcgtcgggc gagttgaggt tcaggaaaaa gagatggctg
     4201 gcgggcaagt ccgcgagcag cgcgaagtcg gccagacgcg tccgcgcctg cgcgacgagc
     4261 acgcgcggcc ggcgctcgcc ctcacgcagc aatcgctcga caatctccag acattgccgc
     4321 ctttcgtaga gcgcgcagag cggctgcaca cgcccgtctt cctgaacggg cacgacggcc
     4381 tccactccct ccttgacgaa agttgtcagg cgactcagaa gccccccggt cacgaaaggg
     4441 aggtcgcacg agacgaccgc gacccacgcc gcctccgcgt gcgtcagcgc agattgaagg
     4501 ccgccgagtg cgccgcagtc gtcgtaaaaa tccgggacga ctgtgaggcc gcacaactcg
     4561 ccgccgtggc gagagctgac gaccctgact gttagggtga tctccgagag cgcgccggcg
     4621 accgtctcga caagagtttg accgccgagg cgaagctgcg ccttgtcggc gcccatgcgt
     4681 gaggacgcgc caccggccag tatgaagcct tcgatgtcga gcatttccgt gtagtgtcag
     4741 caggcgtggg cggaaaataa cacgcgccga cgcgccgcga caaggccgcg ccccgcgggt
     4801 cgcgccgtgc ccggcgacac ggatttaacc tgaagcaacc aaagaggttt cgcatacatc
     4861 attgattaac ccgagttcct tgaccggcgt tcgccgccac ggctatagtc aaatcctgcg
     4921 gcgcgtgcgt caagcaagcc cctgcgacgc tgataacggt cgccgctcat tcccggattc
     4981 ccttcgccga gaaggacagt cttcggcggt ttcgaggtac gtacatgatc agaaggttag
     5041 ccgcactctt cgccgtcacg ctcgcgctct gcggcgcggc cgtggcgcag caaacggtcg
     5101 tcacccccaa gacgggcgac gcggcctact tcccgcttca ggaagtgaag gcggggcaga
     5161 agggcgtggc ctacacggtc ttctccggct ccgtgccgga ggagtttaaa gtggaagtgc
     5221 tcggcgtgtt gccgggctac ccgcagccgc ggcagtcgat catcatcgcg cgcatctcgg
     5281 ggccgctggc cgagcgcacg ggcgtcttcg ccggcatgtc cggctcgccc gtctacatcg
     5341 acggcaagct cgtcggcgcc atcgcctaca gcttcccctt ctcgaaggaa cccatcgccg
     5401 gcatcacgcc catccagcag atgatcgaca tcttcgaggg cggcgccgtg cagagcggcg
     5461 agcgcgacca actcggattc ccgcgcgagc cgcgcgccgt ctccttcgcg cagttgggcg
     5521 gctctgactt cggcgtcgca ctgccgaagt cggggatgac ttcggccacc ttcctcgcgc
     5581 ccgtcgccgc cggctcgccg ctcgccaatc tgctcggaca gcagctcgcg cccatcgcca
     5641 cgcccgtcgt cttcggcggc atctcggccg acacgctcgc gcgcttcgcc ccgcagttgc
     5701 aggcgacggg gctgctcccc gtctccggcg tcggcggcgc gtcgcccatc acgccgctcg
     5761 ccaggttcga ctcgaagacg cttctgggtg gcacgtccgt cagcgtccag ctcgtgcgcg
     5821 gcgactactc catcgccgcc gccggcaccg tcacgcaccg cgacggcgaa cgcatctacg
     5881 cctttggcca cccgttcctc ggcctcggct cggccgacat gccgatgagc gaatcctcgg
     5941 tcgtcaccgt catcccgaac acgctcaact ccttcaagct ctcggtgccc ggccagatgg
     6001 tcggcgccat ctcgcaggat cgcgccacgg gcatctacgg gcagttgggc cgcaacccca
     6061 agatgatccc cgtcaagatt aacctgcaca cgagccgcgg gcgcgtcgag acctacaact
     6121 acgaggtcgc gtcagacgaa ttcctgacgc ccatcctgct caatatcacc gtctacaaca
     6181 ccatctcttc gagcgagcgc agcctcggcg actcgaccgt cagcgtccgc ggtcagatca
     6241 acgtcaaggg tcaggacgcc atcaagctcg aacgccgttt ctcggccgcg ggcgctggaa
     6301 tcgccgcggc cggctcggtg gccgggccgg tggccgcgct catgtcgagc ggcttcgacg
     6361 gcgtcgatct ccagtccatc tcgctcgaca tcgcctcggc cgacgtgcgc cgcgccgcca
     6421 cgctcgaccg catcgccctt gaccgcgccg aggtcgcccg cggcgagacc gtcgaggtgc
     6481 aggcttacat ccgcaccgag tcgggcaagc agttcgtgca acgcatcccc gtgcagattc
     6541 cggcggacgt gcccacgggg ccgctcatgc tcttcgtcgg cgacggcggg agcatccagc
     6601 aggcgtcggc catgcagagc ttcgtcccga aagatttagg gcagctcgtc agcgccatca
     6661 accgcctcaa gaagaacgac cgcctctacg tgaggatgct acgcgtcacg cacggcgcgg
     6721 tcatcggcac cgacgagatg ccgagcctgc cgccctcctt cgtcgccacg ctcaacagcg
     6781 accgcagcag cggcggcttc acgcccacgg ccctttcgcc cctcggcgag caggagctgc
     6841 ccgcggccga gtacgtcatc tcggggcagc agatgatcag catcaacgtc gtgcgctaag
     6901 aagccggcaa gcacagtacc gcgagcggta gcgagcgggt atgcctacga cgagagtcct
     6961 gtctcgcgta tccggacccg cgaggcttcc gctcgcggta ctgtttgtgg cgataaccac
     7021 ggcacgtctg acaactccgg cagatctgtg aggtgaggaa cacggtcagt gcttctcacc
     7081 tcatttttta tccacgacga acagaaaacg tctttccgat tcgggaatga ggacggagga
     7141 aaaagttaat cccgccttct caagttccgc gccgaggagg tgtccgccga acaggagcag
     7201 ccgtttgaca cgcgacgacc acgagaggag ttcgggaata acttccgtga atcgttcgag
     7261 cgcgcggcag gtgagcgcgt cggcggccgg cggcggcgcg ctctggaatc gctcggccag
     7321 cacctcggct gcgtcgcctc tactcacggc gacgagcgcc tcccgcaaaa agacagcctt
     7381 cttccgagac gattccacga gcagggcgcg aaggtcgggg cgcgcgatga gacacgggat
     7441 gacgggcagc cccgcgccgg agccaacgtc aaccacacgc gctcccgccg ggaggtgcgg
     7501 cgcggcaatc agcgattcca gcacgtgacg catggcgaac tcggcgggcg tgcagggcgc
     7561 gaccaagtgc aggcgcgggt tccagcgcat gacgatttca aagtaggcgc gcaggcccgc
     7621 gaccacgccg acgtccgtcc cgaagtcgaa gcgcgctgcg ttcgtctcca acgctttcac
     7681 aaattcctcg gcaggtgtta cgtttccacg gttcatcaat ctgaggttat caagaagacg
     7741 gttctgatgg acagcacaaa gcgattcacc gcggagcgac taggcagcga gcacgcgcgg
     7801 ctcggccgcg aggccgtccg cgtcgtctcc gagtacgccg gctcgctcga ccggctcccg
     7861 gtctgttcgc gcgccacgcc cgctgaactg gaagccctct tcgacgagcc gcttccgctc
     7921 gacagcacgc cggccgcgga aatcttcgcc aagttcgagc gcgacgtgct cccgcacgcg
     7981 atgaacattc ccagcccgcg ctactacggc ctctttaacc cgacgccgct gcccatcgcc
     8041 gtctgggccg acacactcgc ctccgccatc aaccagaacg gcgcggcctg gcgcaactcg
     8101 ccctcggcga gtgtcatcga agcgcgagtg ctgcgctgga tgtgtgaact cttcggtctc
     8161 ggcgcggagt cgttcggcac cctcacaagc ggcggctcgg aagccaatct catcggtctc
     8221 aagtgcgccc gcgaccgcgt ggcaataaag gcgagagacg gcgggctgat ctcggccggg
     8281 gtgacgggca acctgatcgt ttacgcctcc gagcagtctc attattcgct cgtcaagtca
     8341 gcagacatcc tcggcctcgg ccgccagaac ctccgcaaga tcgacacgga cgagcgcttc
     8401 cacatccgtg ttgaccaact ccgcgccgag attgaaaaag atttgcggga agggaacacg
     8461 ccctgctgtg tggcgggtgc ggccggcgcg acctcgacgg gggtcgtcga tccgctggac
     8521 gagctggccg acgtggcggc cgagcacgga ctctggttcc acgtcgatgc cgcatacggc
     8581 ggcggcctcg ccttctccga gaaacataga gtgaggcttc gcggcatcga gcgggccgac
     8641 tccgttaccg tcgatccgca caagtggatg ttcatgccct tcgcgtgcgg cgcgctgctc
     8701 gtgcgcggcg gcggccgggt gctccgcgac tcgttcgaca tcacgcccga gtacctgagc
     8761 gaagaccgcg gcggcgccga cgtggagtac gacttcttcc gctacggaca actcggcacg
     8821 cgccgcttca acgcgttgaa aatctggatg gccctcaggc acatgggcgt cagcggttat
     8881 gcctcggtgg tcgagaggca gatcgatttg gcgcgccacc tcgccgcgcg gctcgacgcg
     8941 ctcgccgact tcgagtgcgt cggcgaggtg gagacggcgc tctgctgcgc gcgtttcgtg
     9001 ccggccgctt tgagagcgcg cggcggggaa gagatcgacg agttgcagaa ggcgcttcag
     9061 cagcgcatcg agcgaagcgg cgaggcgtgg tttgcgacga cggtgctcca cggccgccgc
     9121 gcgctacgca tcaacgtcaa cagcttcctc acggagcgcc gccacatcga cgatctggta
     9181 gaattgctcc ggcgcgaggg cgcgagcctt ttggcggagt ggaagagctg aggggcggaa
     9241 gggcttatga agacttcaaa gttgagtcgg gtcggcgcgc tgttattgtt gtcgctgtgt
     9301 gcggtcgtgc tcgtcgcgtc gccgggcgtg cgggcgcagg agacgccgcc gccgtcgcgg
     9361 gcggaggagg gcgagcggtc tgttgcggcg atcaagtcgc gcgccgagtt cgaccgcgtc
     9421 gcacgcgtct acaccgaccg gccctacgct ttgccgcacg tcctcttcgt catcgaccgc
     9481 aaggacaaga accgcatcta ttacgtcaac tcgaagcgtt accgtttcca caaagacttc
     9541 gtcaacggca cttacctgtc gctggagcgc ggacaggagt ttgagaacaa ctatctgaag
     9601 cccaaccgcc gcttcatcct cggcacgctc gcctatcagg cgcccgtgcg cgcgtggacg
     9661 ttcgagttct gggagggcga cctgatagcg gccgagttgg ttaaattgac ggccgaactg
     9721 attcgacaga cgttctacga gccggtcaaa ttcaagccga actctctgcg gcaggaggag
     9781 gcgtccgtgc aggtcgaggg gctggagcgc gtgctccaga gcgacatctc gcgcgggcag
     9841 gagtatgagg cgctaaacgt cgccaagggc atcggccgca tccacgtcgt cgagaagctg
     9901 gacgagcacg tcgagatcgg cttcaacgag ataatcgtcc tcaacgaagt tccgctgagc
     9961 ctgccgcccg tggccggcgt catcatctcg aagccctcga cgcctctctc gcacatcaat
    10021 ctactggcga agggctgggg cgttcccaac gcctacatca aaaacgcgca cgaactgctc
    10081 aagggttacg acggctggtg ggtgaatttc gagacgtttc ccgacaagta caccatcaag
    10141 cgcgccgaca tcggcgacat caaaaagtat caggacgcgc tcgccgcgga gcacgtcctg
    10201 atgacgccgc gcttcgacct cgcggcaaag agcctgctcg atttgcgacg gcagcgcgcg
    10261 cgcatggcgc cggcttacgg ggcgaagtcg gcgaatctgg gcgaggtgat gagcgcgcgt
    10321 ttgcgcggcg tcacggtgcc gaacggcttc accgtcccca tcttctacta cgaacagttc
    10381 attaaagaga acaagctcga cgacgcgatc cttgagatga tggacgacca gaagtttgtc
    10441 cacgacccgg cctatcgccg cgagcgactc aaggagatgc gcgcgcagat tcaggccggc
    10501 aaggtgaacg aacagctccg cgccggggtg ctgcggcgcg cgcgcgccga gtacgccggc
    10561 aagggcctgt tcgtgcgtag ctcctcgaac gtcgaagacc tgccgaactt cagcggggcg
    10621 ggcctctatg acacggtgcc gaacgtgttg gaggaggaaa agctgatcga agcgatcaag
    10681 acggtgtggg cgtcggtctg gaacttctcg gcctacgagg cgcgcgagcg cgcgcacgtc
    10741 gatcacacga aggcgtttat ggccgtgctc attcaggagg gcatcaacgc cgacagcgcc
    10801 ggcgtgatga tcaccaccga cccttacgac aacgacaacc gcgacgccat ctacatcagc
    10861 gccaagcgcg gactgggcat caaggtcgtc gagggcaagc gcgtcgccga gcaggtcatc
    10921 taccgtccgc gctccgactc cgtacaggtg ctcacgcgct ccgacgagga gagcctgctc
    10981 acgttcgacg agcgcggcgg cgtgcgcgag gtgcccatct cgggcgagcg cgcggtgctc
    11041 acggacgcga tggtgcgccg cctcgcggtg gccgcccggc gggtgcgcgg actcttcgga
    11101 ggccgcgagc aggacatcga gtgggtctat cgcggcgggc tgctctacat cgtgcagtcg
    11161 agaccgttcg tcacaggtgg ttgaaggggg atgattctga aaacgaaaac gcgcgctctt
    11221 attctctcct gtctggccgc aaccctcacc ggctgcaacg acgccccgga gggcacaaac
    11281 cgcgcggcca actccgctgc cacgtcgtcg tcgccggccg ccgagcgtcc cgtcgagggc
    11341 gcgaacctcg acgcggagat acaacggctg gagaagttgg tcgaacgcaa ccccggcgag
    11401 gactccgtga cccgaccgct cgccgccgcc tacgtgcgcc gcggcaacaa cctgcgcgcc
    11461 tcgaacgacc tccgcggggc gctcaaggac taccgcagcg ccatcaagta cgacgaggac
    11521 aacgaggagg cccagaagaa tatcgccgag atcgtcccgc aggtggaggg cgagaagacg
    11581 ggcgagtacg gcgaaccggc gcccctgccc atcaccccca acgtcacgac cgacactgac
    11641 gacaacgccg ggccttcgcc gaccgcgcag ccgtctccta gaaaaaatgt gaatcgtgaa
    11701 tagctgttag ctgttagctg ttagccttca gctaaaagct gtttggaaag ccccgaaggg
    11761 gcgagataca aaagcctggg gcacgccctg gtgcacgagc cgccatcatc ggcaacagta
    11821 ccgcgagcgg tagcgagcgg gtctcgcgta tgcgcggcgg gtctctaatc gtaggcacac
    11881 ccgctcgcta ccgctcgcgg tactgttcgg ggcttcacga acagctaacg gctaacggct
    11941 aacagctcac aaaaactatg aacgacgacg ttttcaaaga tgacatcctg aaggggcgcg
    12001 tggcgctcgt gacggggggc ggcactggga tcacgggcgg cgtggcgcgt gcgctcgcgg
    12061 aggccggcgc gcgcttggcg ctcgtcagcc gcaagttgga gcaccttgag ccggccgccg
    12121 cactcatccg cgaggcaggc ggggaggccg tggccgtcgc ggcggacgtg cgcgactacg
    12181 aacaggtcga gcgggcggtg gccgcgacgg tcgaggcgtt cgggcatctc gacatcgtcg
    12241 tcaacggcgc ggccggaaac ttcctgtgcc gcgccgagga gttgtcgtcg aacggcttcg
    12301 gcacggtcgt ggacatcgac ctcaagggca ccttcaacgt ctgccgcgcg gccttcgacg
    12361 agctgaaaaa gaaccgcggc caaatcctca acatcagcgc gacgctccac tatctcggca
    12421 cgccgctcca gctccacgtc tcggccgcga aggccggcgt ggacgctttg accagaaacc
    12481 tcgccgtcga gtggggcgct tacggcatcc gctcgaacgc cgtcgcgccc ggccccatcg
    12541 aagacaccga aggcatgcgc cgcctcgtcc cgcccgaagt cagggaacgt ctgctgcgcc
    12601 gcatccccct cggccgcttc gggcgcgtcc gcgacgtgga gcggtgcgcc gtcttcctct
    12661 gctccgacgc ggcgagcttc atcaacggcg ccgtcatcgc ggtagacggc ggcaactggc
    12721 tgcacaacaa attggggtaa ggggtaaagg gtaaaggggt aagggaaaga ccgattcttc
    12781 tcccttaccc tttccccttt cccccttacc ccttgccttc aatgatcgac cttcagccat
    12841 cgacatttga agcgttcgag cgcgaagtcg aacgcggcaa cgtcgtcccg gtcgtgcgga
    12901 cggtgctggc cgacttgcag acgcccgtcg gcgcttacat gctccttgcg gcggacgcgc
    12961 cctattcgtt cctcctcgaa tcggtggagg gcggcgagcg catcgcgcgc tattcgttcc
    13021 tcggcttcaa cccggagatg gtcgtcagag gccgtggggc cgacacgttc gtcgagcgcg
    13081 cgggcgttga ggtcgagaca cacgcggggg tgcgcgcgac cgactacctg cgcgaatact
    13141 tccggggtaa gacgctcgcg cggcgcgcgg gtctcgcgcc gatggccggc ggcgccgtcg
    13201 gctgtctcgc ttatgaagcg gcccgctggt tcgagccggt tcttggggac ggc
//
LOCUS       DASVES010000108        15327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1582779, whole genome shotgun sequence.
ACCESSION   DASVES010000108 DASVES010000000
VERSION     DASVES010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582779"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1566
                     /locus_tag="VF240_03900"
     CDS             187..1566
                     /locus_tag="VF240_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7174410.1"
                     /translation="MRHATTKSFRTALTVALFLALVASTHVASTAQAFNKEEFAARRA
                     KLFEQIADGVAVVFAAEPHPHAVKFRQAPDFYYLTGVEEPGAVLLLVGRNKQSFIFAH
                     KRSEIEVQVAGPGIHNLEKPNEFYGLSQVMPLENFLTVFNFASKGAQKFYLPLSAPDS
                     LQKGRGETEQDEAQLASNPLFRYTPPVRQAVARLRELHPGASVADLNPLLDKMRWVKT
                     PYEIERMRQSGRVGAEGVKAAMRGTKPGMFEYEVEAAARFVMTKMGARGHAFTPIVAS
                     GPNSITWHYDRNDRQMQSGDIVLMDYGADFDYYTSDITRTWPVSGTFTPEQEKMYRCI
                     LEARDAIIAAMKPGVTIRQMQDAAEVVYKKHGFLQQFMELNRYVGHPVGLSVHDVTPG
                     PQSTTALEPGVVFNVEPLIEFRDKKIHMRLEDSVLVTATGAENLTAGVPADVEAVYAL
                     IKQKGIGVD"
     gene            1843..2211
                     /locus_tag="VF240_03905"
     CDS             1843..2211
                     /locus_tag="VF240_03905"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX7174411.1"
                     /translation="MSAWTKSVLLFVLNLLDAQLTVVWVEMGVATEGNALMARVLEAG
                     TAHFLLTKLLIGALVAYTLYRFSHLALARRGLGFVLGLYFLLMFVHAAAGMSAMGFHA
                     PDAFVAFVSHLPDRLLALLA"
     gene            2530..3675
                     /locus_tag="VF240_03910"
     CDS             2530..3675
                     /locus_tag="VF240_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7174412.1"
                     /translation="MYRLSARQILAIALLSASFAAGGVLVYDRWLGPSAHPAVSAFEE
                     GTPEGVADPSTVTDEQNNIEVYKSVSPGVVFINTARRPRSFFDQGGQGNGSGSVIDDQ
                     GHILTNNHVVEDADAVTVSFGGDKEYPAKIVGRDPDTDLAVIKVEAPREVLRVVPFGD
                     SDRLIVGQKVLAIGNPFGFDRTLTTGVISGLQRPIQARNGRVIEGAIQTDASINPGNS
                     GGPLLDSHGRMIGINSQIISPSNGSVGIGFAVPISIARRVVPQLITNGEVVRPKLGIA
                     PYNVSDIRGVRLPVQEGVLVFQVYPNTSAAAAGLRGLEQTAEGDMVLGDIITSIDGER
                     VKNSNDLFRILDKRKFNDVVQVEIVRDDQRRTVPVRLLAERRGGTRR"
     gene            3767..4057
                     /locus_tag="VF240_03915"
     CDS             3767..4057
                     /locus_tag="VF240_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174413.1"
                     /translation="MTETEHFVKERFGWVCRRCRDAACGGAAAGVENTGRRASLVEGR
                     ARFFHEGEAEEREPKLSAPGLARRRDAGGQRILFCPICGAEEADSSGEEVVI"
     gene            complement(4346..5038)
                     /locus_tag="VF240_03920"
     CDS             complement(4346..5038)
                     /locus_tag="VF240_03920"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEX7174414.1"
                     /translation="MPAPNENAIGDLVSLLFQAHPWHGVSPGEGAPAVLNAYIEIVPT
                     DAVKYETDKRSGHLKVDRPQRFSSFPPTLYGFIPQSFCGERVAALCEERTGRTGILGD
                     GDPLDVCVLTERPVSHGNLFVRARPVGGLRMIDAVEADDKIVAVLDADVAFGHIESIT
                     EIPSGLIDRLRHYFLTYKQLPQDAPRRVEIADVYDRDEAHDVIRRSLEDYRARFGAPE
                     TRLDELRRLLAT"
     gene            5229..6332
                     /locus_tag="VF240_03925"
     CDS             5229..6332
                     /locus_tag="VF240_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174415.1"
                     /translation="MSAITTAQTQATTTARAARGAVSLRWIIGARDDLVWIIGSVASS
                     YLMFALYVGGVVPLLPMVALWAILIDAPHVFATLSRTYFDREERRARKRLLWWSLLFF
                     AVGPVMVLAGASSALAFFFLAALWAYYHLVKQHYGFMVLYKKKNADLARADNVLDRAF
                     LALAFWLPFVIFIARDPEAMERVPAPLHRAVSYLEWTLIVATAVAATLWLARQLQRAA
                     RREPLNVPKYLLLAAAIPMHWVVLLTPMPHKPIAIVALLTVYHNLQYHRLVWFHNQKY
                     TRGDDRRERFGGAELISRRLAFYVLFGILFGLWYQGPRQYVHWSSGFGQRDLSTQLFS
                     SFFWGYAFIHYYLDSKIWRVRRDPSVGKALHME"
     gene            complement(6400..6681)
                     /locus_tag="VF240_03930"
     CDS             complement(6400..6681)
                     /locus_tag="VF240_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174416.1"
                     /translation="MAQTATATKIDDYTQTTGGATKDVVESTARGLDQTTKQATGISL
                     FHLLTLGSIGASIALYIAGKKDAAIFVGLWPPTFQALKAVADTNKEDKK"
     gene            complement(6825..7298)
                     /locus_tag="VF240_03935"
     CDS             complement(6825..7298)
                     /locus_tag="VF240_03935"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7174417.1"
                     /translation="MRFVKESVIRARPERVFAFHELPDALRRLTPPWESARVIEHAPS
                     LQAGARAIVETRVFGVVPVRWVALHTAYEPPRMFEDVQVSGPFRSWRHRHTVEPHAEG
                     ATLRDEVEYEPPLGPLGRALAPLLVEPRLRRMFDYRHEVTREWCEGDAGGDSIKG"
     gene            7446..8318
                     /locus_tag="VF240_03940"
     CDS             7446..8318
                     /locus_tag="VF240_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX7174418.1"
                     /translation="MSQMIEKYLTTRQLARLWLVSEATVKRWADAGLLPARRTAGGHR
                     RFLPNEVARFQTERGLGRTAAVAAVSAGAGLERAGRNVGVDSELFFNAIVAGHDDAAT
                     LLIEAYLDGVEMWRLFDETVARAMHRVGELWHEGSVTVADEHFATRTTTRAVERLGVS
                     VRRREGRGLSAICCAPEGELHELPVLCLQVLLESEGWRVRGLGANTPFFTLADAVERH
                     RPDLVCISATMLLDLERSAREYAQFVEAARRAGARVALGGQGFRDEAARRRFDAEFHG
                     ESFEKLMNFAGSVK"
     gene            8321..8815
                     /locus_tag="VF240_03945"
     CDS             8321..8815
                     /locus_tag="VF240_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX7174419.1"
                     /translation="MQGAQQTGLVQALALGASVAVCFAAAAIGSAFTAPAIGAWYARI
                     AKPAWTPPNWLFGPVWTALYLSMAVAAWLVWRRAGLAGARLPLSIFGVHLVLNALWSI
                     LFFGLQRPAIAFAEIILLWASILATILSFYRVAPVAGLMLIPYLLWVSYAAALNFSIW
                     KLNS"
     gene            complement(8855..11491)
                     /locus_tag="VF240_03950"
     CDS             complement(8855..11491)
                     /locus_tag="VF240_03950"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX7174420.1"
                     /translation="MPDREIAGLEKYLERIWEVARRNGLDPFPTRFEIVPATVMYEVG
                     SYALPGRYSHWTFGKAYHRMKTMYDFGLSKIYEVVINTNPAYGFLLETNSPVQNKLVM
                     SHVLGHVDFFKNNAYFSKTNRRMVDSASSHAARMSEYEFKYGRKPVERLLDAVLSIEE
                     HIDPNFFIRREREGGEEPGDRGEWKQPKQGPYDDLWSLERRGEPAAAEADPVKSRGER
                     LPEKDLVYYIMRNSPTLVDWQRDLLAMIREEMEYFVPQMQTKTLNEGWACLTSDALIL
                     TERGFLHFDELVEAGEKIRVAVGGVNELQRVTDFHKEELMPTVRVRTRRGLTVEGALK
                     HRIRLAGGAWAYLKDLKVGDRLAVACGLNVWPKKHQSLGFTPSEPTPTLNDVAAVADT
                     SVWSVLRHMGGARTVRATEIDAALRLISYQPHNARRVLTTRTPLQVGSILTEEIARLL
                     GYFVGDGCKTKSGICLTTGDGEYAAELARTMRESFGLPSSVYWAASEVGGRWRVEVHS
                     RELLRLLESVGIDLQDKARSKRVPSAILRSPKSVMSAFLRGYFDADAYAGPEGVRLSS
                     SSAELIGTVQTVLLNYGILSRQRRHPHDITQLEICGASAALFLEEIGFSLDRKQRALR
                     EYVAGHRWFRREELADEVVSVEPGLADVYDITVDERHAYVANGFVNHNSFWHARIMRE
                     LDLPDEEMLEFAELHASVVSPHKGQLNPYYLGYKIFEDIERRWDSPSAGEREKFGREG
                     GAGRAKIFEVRELDNDVSFLRNYLTEELCEELDLFVYELVDEEEWTVTEKRWERVRDQ
                     LVGNMTNFGFPYIVVADGDYNGNRELYLKHQHEGAELDAPYARKVLEYVHTLWGRPVH
                     LETTVDEERVVMHYNGEDHDED"
     gene            11934..15089
                     /locus_tag="VF240_03955"
     CDS             11934..15089
                     /locus_tag="VF240_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7174421.1"
                     /translation="MISKIKTLSSAFAFMLLLTVAAFGQETTGNIEVTVTDPSSARVP
                     GASIVITGGSVNRTVTADEQGFARIQQLQPGIYTVAVSGGNFRAVNFENVEVVLGKTT
                     PINAQMQAGGIMEAVEVSASDVAPIDPTDNTIQTNITERTAELLPKGVNFTSLLQTAP
                     AVRNEPLSGGFQIDGASGSENTFIIDGQEVTNFRTGTLNSNNNIPFQFVQEVQIKSSG
                     FDAEFGGATGGVISVATRGGSNEWRGEFGMEFRPSGLQGTPRPFQRLTTVAASTGVTA
                     LTTTEYIPVRKDAGTDVFPTASLGGPIIKNRAWFFGSFSPHFLHTDRTIEYVTNDPRT
                     RTVTNQQTYTSRQRNEYTFARIDLQPFNTLRMTGTYLWNPIDLRGILPATTTVFDTTL
                     PGVTFPGRGRVQGSEYINNTGGRQSSNNTTGSIIWTPNEKLVLTARGGYSFLNEKLGN
                     YGIPGVIGQTRHIVQATTTAPPAGFGLGVGQTFPSFTQILFDASRRRTFDVDASYLVN
                     NFGGRHQFKGGVQFNGISNQIQSTLVDTVVIRFGTDQTIESLTGHDFDTTPGAIGAGW
                     LQRFGAQGAAGSNNLAFYIQDSWQPTSRLTLNLGLRTERENSPSFNELGEGITFDFMD
                     KLAPRLGFAYDLTGDGKTKLFGSYGQFYDRFKYELPRGSFGGNFFRNDYFEIFAGQTV
                     NQFNRQSIIGGNGDPIGGTCPIPSSQGFSRCQLDFRIPSNLPGQPDFGQVDPDIKAFR
                     QSEFTIGLERDLGMYGLLFRGRYTHKQVDVAVEDIGIVVPGGEAYVIGNPGRGLAAEI
                     AEANGVLTLDAVRDYDAVELNIDKRFTDRFYFNSSYTWSRLFGNYAGLASSDEAGRTS
                     PNVNRNFDLPFIGFSALGRPDNGLLATDRPHSFKFSGAYEQRWNSTNSTEISGFQIIQ
                     SGTPITTRFTFHGVAGQILNGRGDLGRTEAFTQTDLGLRHRYRFGNDGRFAFVANVDI
                     LNLFDEENVLGHFEAISATGVDTDNIGLATGNPGFLLYQTTNTSTAFNQVLNATRDPR
                     YNLPNFFQGARSVRFGFKLQF"
BASE COUNT         2724 a         5046 c         4638 g         2919 t
ORIGIN      
        1 gaatttcttt ccaacatcct ggaaagaaat tcaacttgag cgtcgcgccc gcgtcttttc
       61 tccgcccggc ctgctcccgg acgaaaccgt gcgccgcccg ccgccgtatg tcactcttgc
      121 cattgccgcc acggctgaac gcacgagctt tccactcgaa accttcacac aggagtagac
      181 acgcgcatgc gccacgcaac gaccaagtct tttcgcaccg ccctcaccgt cgcactattt
      241 ctcgccctcg tcgcctcaac gcacgtcgcc tccacggcgc aagccttcaa caaggaggag
      301 ttcgccgcgc ggcgcgcgaa gctcttcgag cagatcgccg acggcgtcgc cgtggtcttc
      361 gccgccgagc cgcatccgca cgccgtcaag ttccgtcagg cgcccgactt ctattaccta
      421 acaggagtgg aggagccggg cgccgtgctc ctcttagtcg gccgcaacaa acagagcttc
      481 atcttcgcgc acaagcgctc ggagatagag gtgcaggtcg ccggccccgg catccacaac
      541 cttgagaagc cgaacgaatt ttacgggctg tcgcaggtca tgccgctcga aaatttcctg
      601 accgtcttca atttcgcctc gaagggcgcg cagaagtttt accttccgct ctcagcgccc
      661 gacagtctcc agaaggggcg cggcgagacc gagcaggacg aggcgcagtt ggcgtcgaac
      721 ccactgttcc gctacacgcc gcccgtccgt caggccgtcg cgcgcctgcg cgagctgcat
      781 cccggcgcat cggtcgccga cctcaacccg ctcctcgaca agatgcgctg ggtgaagacg
      841 ccctacgaga tcgagcgcat gcgccagagc gggcgcgtcg gcgccgaggg cgtcaaggcg
      901 gccatgcgcg ggacgaagcc ggggatgttc gagtatgagg tcgaggccgc ggcgcgcttc
      961 gtgatgacca agatgggcgc gcgcggacac gccttcacgc ccatcgtcgc ctcggggccg
     1021 aacagcatca cctggcacta cgaccgcaac gaccggcaga tgcagtcggg cgacatcgtc
     1081 ctcatggact acggcgccga cttcgactat tacacctccg acatcacgcg cacgtggccc
     1141 gtctccggca cgttcacgcc cgaacaggag aagatgtatc gctgcatcct cgaagcccgc
     1201 gacgcgatca tcgccgcgat gaagccgggc gtgaccatcc ggcagatgca ggacgcggcc
     1261 gaggtcgtct ataagaagca cggcttcctc cagcagttca tggagctgaa ccgctacgtc
     1321 ggccaccccg tcggcctctc cgtccacgac gtgacgcccg gcccgcagtc caccacggcg
     1381 cttgagccgg gagtggtctt caacgtcgaa cccctcatcg agtttcgcga caagaagatt
     1441 cacatgcgtc tcgaagactc cgtgctcgtc accgccaccg gcgccgagaa cctgacggcg
     1501 ggcgtgccgg ccgatgtcga agccgtctac gcgctcatca agcagaaggg catcggggtg
     1561 gactaaaagc cgtcattcgt caatcgtgaa tcgtaaatcg tgtttgaggt cggctcaact
     1621 tgaagcggtg aatcgcaagt cgggtgacgg gcaatcgatt gacgatttac gattgacgat
     1681 tcacggtttt tgaatttccc cgacctttag aaatttactt cagacctaag cgagaaatcg
     1741 cgttcctccc gtgtgaaacg agacccccaa gttttcggac ttgggggttt cgtacttaag
     1801 ggacgtgttt aaacaaagtt ggccgtaagg agctaaccct cgatgagcgc ctggaccaaa
     1861 agcgtgctgc tgttcgtgct gaacctgctc gacgcgcagt tgaccgtcgt ctgggtggag
     1921 atgggcgtgg cgaccgaggg caacgccctg atggcgcgcg tgctcgaggc ggggacggcg
     1981 cacttcctcc tcaccaaact cctcatcggc gcgctcgtcg cctacacgct ctaccgcttc
     2041 tctcacctcg ctctggcccg gcgtggtctc ggcttcgtgc tcggtctcta cttcctgctg
     2101 atgttcgtcc acgcggcggc cggcatgtcc gccatgggct tccacgcccc cgacgccttc
     2161 gtcgccttcg tctcacacct gcccgacaga ctgctcgcgc tcctcgcgta gcgaaaaccg
     2221 tctcccggat ttataaccac gcgcggcctc tgctttttct gagcacggcc gtccactctt
     2281 acgccgaacg cggaactttt ccgtcgaatc cctgtaatcc atttcatggg tctttgtgcg
     2341 cgcgtggagg cgtcgcgcgg cactcgtttg aacccgactc ggcgcgcgct cgtaaaaagg
     2401 gtaagatgct gccggcgcaa agtccgccgg agaaggtcgc cgcgcaacgc ctttcgccct
     2461 cgcgggcatc ttaaagaggt ggacaacgtg ggccgcgaaa gtcatcaatt cataagtaga
     2521 gataaagcca tgtacagact ctcggcacgt cagattttag ccatcgcgct gttgtcggcg
     2581 agcttcgccg cgggcggcgt cctcgtgtat gaccgctggc tcggcccaag cgcgcaccct
     2641 gcggtgtcgg ccttcgagga ggggacgccg gagggcgtcg ccgacccctc gaccgtcacc
     2701 gacgagcaga acaacatcga ggtctacaag tcggtctcgc ccggcgtcgt cttcatcaac
     2761 acggcgcgcc ggccgcgcag cttcttcgac cagggcgggc agggcaacgg ctccggctcc
     2821 gtcatcgacg atcagggcca catcctgacc aataaccacg tcgtcgaaga cgccgacgcc
     2881 gtcacggtca gcttcggcgg cgacaaggaa tacccggcca agatcgtcgg tcgcgacccc
     2941 gacaccgacc tcgccgtcat caaggtggag gctccgcgcg aggtgttgcg cgtcgtcccc
     3001 ttcggcgact ccgaccggct catcgtcggc cagaaggtct tagccatcgg caaccccttc
     3061 ggcttcgacc gcacgctcac caccggcgtc atctccggcc tccaacgtcc catccaggcg
     3121 cgcaacggcc gcgtcatcga aggcgccatc cagaccgacg cctcgatcaa ccccggcaac
     3181 tcgggcggcc cgctgctcga ctcgcacggg cgcatgatcg gtatcaactc gcagatcatc
     3241 tcgccgtcca acggctcggt cggcatcggc ttcgccgtgc ccatcagcat cgcccggcgc
     3301 gtcgtgccgc agctcatcac gaacggcgag gtcgtccgtc ccaagctcgg gattgcgccc
     3361 tacaacgtct cggacatccg cggcgtgcgc ctgcccgtgc aggagggcgt gctggtcttc
     3421 caggtctacc ccaacacttc ggctgcggcc gccggcctgc gcggactcga acagaccgcc
     3481 gagggcgaca tggtcttagg cgacatcatc acgtccatcg acggcgagcg cgtcaagaac
     3541 tcgaacgacc tcttccgcat cctcgacaag cggaagttca acgacgtggt tcaggtcgag
     3601 atcgtccgcg acgaccagcg ccgcaccgtt cccgtgcgcc tgctcgccga gagacgcggc
     3661 ggcacacgac gttaataaga gaagtcagag gtcagaggtc agaggtcagt aaagagcatc
     3721 ttgtctttca ctgacctcta acctctaacc tctgacctct ttttttgtga ccgagacgga
     3781 acacttcgtt aaagaacgct tcggctgggt gtgcaggcgt tgccgcgacg cggcgtgcgg
     3841 cggcgcggcg gcgggcgttg agaacacggg gcggcgcgct tctcttgttg aggggcgcgc
     3901 ccgttttttt cacgagggtg aggccgagga gcgcgagccg aaactctccg caccgggact
     3961 cgcgcgccgg cgcgacgcgg gcgggcagcg cattctcttc tgcccgattt gcggcgcgga
     4021 ggaagccgat tcctccggcg aagaggtggt gatataagtc tgcacgtcgg gagcaatttc
     4081 aaggagccgc ggagcggcgg aagaatgtag cccacggcgt aagccgtggg gacaggtgcc
     4141 agaaaacatt ctaagccccg taggggcgaa agaatctcga cgctgagcac gatgcccgtc
     4201 gagattcttt cgcccctacg gggctttcat cgtcgtgctt gtccgttacc cacggctggc
     4261 gccgtgggct acattcttcc gccgcctccg gcggctggtt gaatccgcga atcggcgctg
     4321 gcggagtcac taaccgggcg gcgaatcacg ttgccagcag ccggcgcagt tcgtcgaggc
     4381 gcgtctcggg ggcgccgaag cgggcgcggt agtcttcgag gctgcggcgg atcacgtcgt
     4441 gcgcctcgtc gcggtcgtac acgtcggcga tctcgacgcg gcgcggcgca tcctgcggga
     4501 gttgcttgta ggtgagaaag tagtggcgca ggcggtcgat gaggccggaa ggaatttccg
     4561 tgatggactc gatgtgaccg aaggccacgt cggcgtcgag cacggcgacg atcttgtcgt
     4621 cggcctcgac ggcgtcgatc atccgcaggc cgccgacggg acgcgcgcgg acgaagaggt
     4681 tgccgtgaga gacgggccgc tccgtgagca cgcacacgtc caacgggtcg ccgtcgccga
     4741 ggatgccggt gcggcccgtg cgctcctcgc agagcgccgc cacgcgctca ccgcagaagc
     4801 tctgggggat gaagccgtag agcgtcggcg gaaacgaaga gaagcgctgc gggcggtcaa
     4861 ccttgaggtg gcccgaacgc ttgtccgtct cgtacttgac ggcgtcggtc gggacgatct
     4921 cgatgtaagc gttgagcaca gcgggcgcgc cctcgccggg cgacacgccg tgccacgggt
     4981 gcgcctgaaa gaggagcgac acgaggtcgc ctatcgcatt ctcgttggga gcaggcatcg
     5041 ttcgaaggcc ctccgcatct gaggcgccgg ccgcgatacg gccgtcagtt tcggactgtg
     5101 ataaaatcgc tcgccgcgcc ggtgttcact gatcgtttca gagctttttc agaatcggga
     5161 ttatgaacgc ctcggcccgg ccgtgtccac gagggcggcc gaggcgcgct atcgtaagac
     5221 cacttctcat gtcggctatc accaccgcac agacgcaagc gacgacgacc gcccgtgccg
     5281 cgcgcggagc tgtttccctg cgctggatca tcggcgcgcg cgacgatctg gtgtggatca
     5341 tcggctcggt ggcgtcgagc tacctgatgt tcgcgctcta cgtcggcggc gtggtgccgc
     5401 tgctgccgat ggtcgcgctg tgggctatcc tgatcgacgc cccgcacgtc ttcgccacgc
     5461 tctcgcgcac ctactttgac cgcgaggagc gccgcgcgcg caagcgtctg ctgtggtggt
     5521 cgctcctctt cttcgcggtc ggccccgtga tggtgctcgc gggcgcgtcg tccgccctcg
     5581 ccttcttctt cctcgcggcg ctgtgggctt actaccacct cgtcaaacag cactacggct
     5641 tcatggtgct ctacaagaag aagaacgccg acctcgcgcg cgcggacaac gtgctcgacc
     5701 gcgcattctt agcgctggcc ttctggctcc cgttcgtcat cttcatcgcg cgcgaccccg
     5761 aagcgatgga gcgcgtgccg gcgccgctcc accgggccgt ctcgtatctg gagtggacgc
     5821 tcatcgtcgc cacggccgta gcggcgaccc tctggctcgc gcggcagttg cagcgcgccg
     5881 cgcggcgcga gcctctgaac gtgccgaagt acctgctgct cgcggcggcc atcccgatgc
     5941 actgggtcgt gctgctgacg ccgatgccgc acaagccgat tgccatcgtc gccctcctca
     6001 ccgtctatca caatctccag tatcaccgtc tcgtctggtt tcataaccag aagtacacgc
     6061 gcggcgacga ccgccgggag cgtttcggcg gggccgaact catcagccgg cggctcgcct
     6121 tctacgtcct cttcggcatc ctcttcggac tctggtatca ggggccgcgc cagtacgtcc
     6181 actggtcatc aggcttcggg cagcgcgacc tctcgacgca gctcttctcc tccttcttct
     6241 ggggctacgc cttcatccac tattacctcg actcgaaaat ctggcgcgtc cgccgcgacc
     6301 cctcggtcgg caaggctctg cacatggaat aagaaaaggg tcgcgccctc ttcagtgcgc
     6361 gaccctttcg acccacccct tgctcgttcg gaaaaaactc tacttcttgt cttccttgtt
     6421 cgtgtcggcg accgccttga gcgcctggaa ggtcggcggc cagaggccga cgaagatcgc
     6481 cgcgtccttc ttgcccgcga tgtagagcgc gatggaggcg ccgatggagc cgagcgtcag
     6541 caggtgaaag aggctgatgc cggtcgcctg cttggtcgtc tggtcgaggc cgcgcgcggt
     6601 cgattcgacc acgtccttcg tcgcgccgcc cgtcgtctgt gtgtagtcgt cgatttttgt
     6661 cgccgttgct gtctgtgcca tgttggtgtt tcctcctctg tccgttgtcc gttgcctgta
     6721 gtttattctg cgcgcccctt gaggtacgca attacgaaaa gcaatgcgag cgagaagccc
     6781 gcgattaaaa aacccttcgt cctgtcaact tccgttctca tctctcaccc cttgattgag
     6841 tcgccgcccg cgtcgccctc gcaccactcg cgagtgactt cgtggcggta gtcaaacatc
     6901 cgccgcagcc tcggttcgac gaggagcggc gcgagcgccc tgcccaaagg cccgagcggc
     6961 ggctcgtact cgacctcgtc gcgcagcgtc gcgccctccg cgtgcggctc gaccgtgtgc
     7021 cggtgtcgcc agctccgaaa ggggccggac acctgcacgt cctcgaacat ccgcggaggc
     7081 tcgtaggccg tgtgcagcgc cacccagcgg acgggaacga ctccgaacac ccgcgtctcg
     7141 acgattgctc gcgcgcccgc ctgcaaactc ggcgcgtgct cgatgacgcg cgcactttcc
     7201 cacggcggcg tcagtcggcg gagcgcatcg ggcagttcgt ggaacgcaaa cacccgctcg
     7261 ggccgggcgc ggatcacgct ctctttgacg aacctcattt caatgcaccc atcatagcaa
     7321 attcgctcaa atcgttcatc acggaaccgt gcttattttc cggcataacc ttgtcttgcg
     7381 ggtttcgaga aagttcttga cacggcgggc gagagcgagt atgtctgcaa ccgcacgaac
     7441 cgctcatgag ccagatgatc gaaaagtatc tgacgacgcg gcaactggcg cgtctgtggc
     7501 tggtcagcga ggccacggtc aagcgctggg ccgacgccgg gctgcttccg gcgagacgca
     7561 cggcgggcgg ccaccgccgc tttctgccca acgaggtggc gcgcttccag accgagcgtg
     7621 gcctcggccg cacggccgca gtcgccgccg tgtctgcggg cgccgggctt gaacgcgccg
     7681 gtcgcaacgt cggcgtcgat tcggagcttt tcttcaacgc catcgtcgcc ggccacgacg
     7741 acgcggccac gctcctgatc gaagcctatc tcgacggcgt cgaaatgtgg agactcttcg
     7801 acgagacggt ggcgcgcgcg atgcaccgcg tgggcgagtt gtggcacgag ggcagcgtga
     7861 cggtggccga cgagcacttc gcgacgcgga cgacgacgcg cgccgtcgag cgtctcggcg
     7921 tctctgtgag gcgcagggag gggcggggcc tgagcgccat ctgctgcgcg ccggagggcg
     7981 agctgcacga gctgcccgtg ctctgcctgc aagtgttgct ggagagcgag ggctggcgcg
     8041 tgcgcggcct cggcgccaac acgcccttct tcactctggc cgacgccgtc gagcggcacc
     8101 gccccgacct cgtctgcatc tcggcgacga tgctgctcga cctcgaacgc tcggcgcgcg
     8161 agtacgcaca gttcgtcgag gcggcccggc gcgcgggcgc gcgcgtcgcg ctcggcggac
     8221 aggggtttcg agacgaagcc gcacgccgga ggttcgacgc ggaatttcac ggggagagtt
     8281 ttgagaagtt gatgaacttc gccgggagtg tgaagtagag gtgcagggcg cacagcagac
     8341 gggtctcgtt caggcgctcg cgctgggcgc gtcggtggcc gtctgcttcg cggcggcagc
     8401 cattgggtcg gcgttcaccg ctccggccat cggcgcctgg tacgcgcgca tcgcgaagcc
     8461 cgcgtggacg ccgcccaact ggctcttcgg ccccgtgtgg acggcgctct acctgtcgat
     8521 ggccgtggcg gcgtggctcg tgtggcgccg cgccggactt gcgggcgcgc ggctcccgct
     8581 ctcgatcttc ggcgtacacc tcgtcttaaa cgccctgtgg tcgatcctct tcttcggctt
     8641 gcagaggccc gccatcgcct tcgccgaaat catcctgctg tgggcgtcca tcctcgccac
     8701 catcctgtcg ttctaccgcg tcgcacccgt agccggcctc atgctgattc cctacctgct
     8761 gtgggtgagc tatgccgcgg ccctcaactt ctcaatctgg aaactgaatt cttaagacgc
     8821 gcgccggccc ctcagtcctg acggcgggct gctttcaatc ctcatcgtga tcttcgccgt
     8881 tgtagtgcat cacgacgcgc tcttcatcga cggtggtttc gagatggacg gggcggcccc
     8941 agagcgtgtg aacgtattcg agcaccttgc gggcgtaggg ggcgtcgagt tcggcgccct
     9001 cgtgctggtg tttgagatag agttcgcgat tgccgttgta atcgccgtcg gcgacgacga
     9061 tgtaggggaa gccgaagttg gtcatgttcc cgacgagttg gtcacgcacg cgctcccagc
     9121 gcttctccgt caccgtccac tcctcttcgt ctacgagttc gtagacgaag aggtcaagct
     9181 cctcacacaa ctcttcggtg aggtagttgc gcagaaacga gacgtcgttg tcgagttcgc
     9241 gcacctcgaa aatcttcgcc cgcccggccc cgccctcgcg gccgaacttc tcacgctccc
     9301 cggccgaggg gctgtcccag cgccgctcga tgtcttcaaa aatcttgtag ccgaggtagt
     9361 aaggattgag ctgacccttg tgcggcgaga cgacgctcgc gtgaagctcg gcaaactcca
     9421 gcatctcctc gtccggcagg tctaactcgc gcatgatgcg cgcgtgccag aaggagttgt
     9481 gattgacgaa gccgttggcg acgtaggcgt gccgctcgtc caccgtgatg tcgtaaacgt
     9541 cggccagccc cggctcgacg gagacgacct catccgccag ctcttcccgc ctgaaccacc
     9601 gatgccccgc gacgtattcg cgcaacgcac gttgcttgcg gtcgaggctg aagccgatct
     9661 cctccaggaa aagcgcggcc gaggcgccgc agatttcaag ctgcgtgatg tcgtgcgggt
     9721 gcctgcgctg acgcgagagg atgccatagt tgagcaggac ggtctgcacc gtcccgatca
     9781 gctccgcgct ggaagaggag aggcggacgc cttcggggcc ggcgtaggcg tcggcgtcga
     9841 agtagccgcg caggaaggcc gacatcacgg acttcggcga gcgcaagatc gcggagggca
     9901 ctcgcttgct tcgtgccttg tcttgcaagt caattccaac ggactctaac agcctcagga
     9961 gttcgcgcga atgcacttca acgcgccacc ggccacccac ttcggacgct gcccaataaa
    10021 cagacgaggg caaaccaaaa gactcccgca tcgtccgggc cagttcggcg gcgtattcac
    10081 cgtccccagt ggtcagacaa atgcctgatt tggtcttgca cccgtcgccg acgaagtagc
    10141 cgagcagccg cgctatttcc tccgtcagaa tgctgccaac ctgcaacggc gtgcgcgtcg
    10201 tgagcacacg cctcgcgtta tgcggctggt aagaaatgag gcgcaaggcg gcgtcaattt
    10261 ccgtcgcgcg aactgtcctt gccccgccca tatgtcgcag cacagaccat accgacgtgt
    10321 ccgccaccgc agccacgtcg ttcaaggttg gcgtcggctc cgacggcgtg aagccgaggg
    10381 actgatgttt cttcggccaa acattcaacc cgcaggcgac ggcgagccga tccccgacct
    10441 tgaggtcttt aaggtacgcc cacgcgccgc ccgccaatct gatgcgatgc ttgagcgccc
    10501 cctcaaccgt cagaccgcgc cgggtgcgga cacgcactgt cggcatcaat tcttctttgt
    10561 gaaagtccgt cacccgttga agctcattaa cgcctccgac agcgacgcgg atcttctcac
    10621 ccgcttcgac cagttcatca aaatgcaaaa agcctcgctc ggtgagaatc aatgcatctg
    10681 aggtcagaca cgcccagcct tcatttaatg tcttcgtctg catctgcggg acgaagtatt
    10741 ccatctcctc gcggatcatg gcgagcaggt cgcgctgcca gtctacgagg gttggcgagt
    10801 tgcgcatgat gtagtagacg aggtccttct cggggaggcg ctcgccgcgc gacttcacgg
    10861 ggtcggcctc ggccgcggca ggctcacccc ggcgttcgag gctccagagg tcgtcgtagg
    10921 ggccttgctt gggctgtttc cactcgcccc ggtctcccgg ttcttcgccg ccctcccgct
    10981 cgcggcggat gaagaagttc gggtcgatgt gctcctcgat ggagaggacg gcgtcgagga
    11041 ggcgctcgac gggcttgcgg ccgtacttga actcatactc gctcatgcgc gcggcgtggc
    11101 tggaggccga atccaccatg cgccggttgg tcttcgagaa gtaggcgttg tttttgaaga
    11161 agtccacgtg gccgagcacg tgcgacatga cgagcttgtt ctggacggga ctgttggtct
    11221 ccaaaagaaa accgtaggcc gggttggtgt tgatgacgac ctcgtaaatc ttcgacagcc
    11281 cgaagtcgta catcgtcttc atccggtggt aggccttgcc gaaagtccag tgggagtagc
    11341 gccccggtag ggcgtaggag ccgacctcgt acatgaccgt ggcgggcacg atctcgaagc
    11401 gcgtcgggaa ggggtcaagc ccgttgcggc gcgcaacctc ccagatgcgc tccagatact
    11461 tttcgaggcc ggcgatttca cggtcgggca taatcgtcat tctcccccaa gagacctcgc
    11521 cccgcaagac acatctcgtc cgacgcctcg gcagacgcgc tctctggcac gactactgtc
    11581 tccttacttg aaaatcaaac atcgggcctg cggccgcccc tcgcccgtcc ggccgcgtgc
    11641 gaccaccacg ggcgggcact cgtggccaac gaagtttttc cgaaattatg gaagagacat
    11701 ccaaaatttt gggagaaagg gcatcgagag gcttcttaag cgaccgccgc cgagccgcgt
    11761 cttccctact gttttcaaca ggatacgaat tattaagatt taattcaggg ttgtggttcc
    11821 gtatttgcat cggggtggga tgtgatattc ccttgcaggc attgagtgat tgaacgaagg
    11881 cctgcgacag tgtcccttca acgaagattt ccgaaatttg aggagcaaac agaatgatta
    11941 gcaagattaa gacgttgtct tctgcgttcg cctttatgct tctcctgacc gtggcagcat
    12001 ttggtcagga gacgacaggc aacatcgaag tcaccgtgac ggacccgtcg agcgcgaggg
    12061 ttccgggcgc cagtatcgtc atcacgggcg gttcggttaa ccgcaccgtg acggccgatg
    12121 aacagggttt cgcccgcatc cagcagctac agcccggcat ctacaccgtg gccgtcagcg
    12181 gcggcaactt ccgcgcggtg aacttcgaga acgtcgaggt cgtcctcggc aagaccactc
    12241 ccatcaacgc gcagatgcaa gccggcggca tcatggaggc cgttgaggtc tccgcgagtg
    12301 acgtggcgcc catcgacccg accgacaaca ccatccagac aaacatcacc gagcgcaccg
    12361 cggagttgct gcccaagggc gtcaacttca cgagtttgct gcagacggcc ccggccgttc
    12421 gcaacgagcc tttgtccggt ggtttccaga ttgacggcgc ctccggctcc gagaacacgt
    12481 tcatcatcga cggtcaggaa gtcaccaact tccgtaccgg caccttgaac tccaacaaca
    12541 acattccgtt ccagttcgtc caggaagtcc agatcaagtc ctccgggttt gatgcggagt
    12601 tcggcggcgc gacgggcggc gtgatcagcg tcgcgacccg tggcggcagc aacgagtggc
    12661 gcggcgagtt cggtatggag ttccgcccgt caggcttgca gggcactccg cggccgttcc
    12721 agcgcctcac cactgtggcc gcatcgaccg gtgtcacggc cttgacgacc actgagtaca
    12781 tccccgtgcg caaagacgcc ggcaccgacg tgttcccgac cgcatctctg ggcgggccca
    12841 tcatcaagaa ccgtgcatgg ttcttcggca gcttctcgcc gcacttcctg cacactgacc
    12901 ggacaatcga gtacgtcacg aacgacccgc gcacccgcac ggtcaccaat cagcagacgt
    12961 acacctccag gcagaggaac gaatacacct tcgcccgtat cgatcttcag cccttcaaca
    13021 ccctccggat gaccggcacc tacctctgga acccgatcga tctgagaggc attctgccgg
    13081 cgacgacgac cgtgttcgac acgacactgc ccggcgtgac gttccccggc aggggccgcg
    13141 tgcagggttc tgagtacatc aacaacacgg gcggccgcca gtcttcgaac aacaccacgg
    13201 ggtcgatcat ctggacgccg aacgagaagc tcgtcctgac cgctcgcggc ggctacagct
    13261 tcctcaacga gaagcttgga aactatggta ttccgggcgt catcggtcag actcggcata
    13321 tcgttcaggc caccacgacc gctcctccgg ccggttttgg ccttggcgta ggacaaacct
    13381 tccccagctt tacccaaatt ctctttgacg cttcgcggcg tcgaactttt gacgtcgatg
    13441 ccagctacct cgtgaacaac ttcgggggcc gccaccagtt caagggcgga gtccagttca
    13501 atggcatcag caatcagatt cagagcacgc tcgtcgatac agtggtcatc cgcttcggca
    13561 cggatcaaac gattgagtcc ctcacgggcc acgacttcga cacgactcct ggagccatcg
    13621 gcgctggctg gcttcagcgt ttcggcgctc aaggcgcggc ggggagtaat aacctggcct
    13681 tctacatcca ggactcctgg cagccgacca gccgcttgac cttgaacctg ggtctgagga
    13741 ctgagagaga aaattcgccc agcttcaacg aactcggcga aggcatcacg ttcgatttca
    13801 tggacaagct cgccccgaga ctgggctttg cctacgacct gaccggcgac ggcaagacca
    13861 agctcttcgg cagctacggt cagttctacg accgcttcaa gtatgaattg ccgcgcggct
    13921 ctttcggcgg caacttcttc cgcaacgact atttcgagat cttcgccgga cagacggtta
    13981 atcagttcaa ccgccagtcc atcatcgggg gcaatggcga cccgatcggc ggcacctgcc
    14041 cgatcccgag cagccagggc ttcagccgct gccagctcga cttccgcatc ccgtccaacc
    14101 ttccgggcca gccggatttt ggtcaggttg acccggacat caaggctttc cggcagagcg
    14161 agttcacgat tggcctggaa agagacctcg gaatgtacgg tcttctgttc aggggccgct
    14221 acacccacaa gcaggttgac gtcgccgtcg aagacatcgg catcgtcgtt cccggcggcg
    14281 aggcttacgt catcggcaac ccgggccgcg ggttggcggc ggagatcgcc gaggccaacg
    14341 gcgtcctgac cctcgacgcg gttcgcgact atgacgccgt cgagctgaac atcgacaagc
    14401 ggttcaccga ccgcttctac ttcaactcca gctacacctg gagccgtctg ttcggtaact
    14461 acgccggtct cgccagctct gacgaagcgg gccgtaccag cccgaacgtg aaccgtaact
    14521 tcgacctgcc gttcatcggc ttcagcgccc tcggccgacc ggacaacggg cttctggcga
    14581 ccgatcgtcc gcactcgttc aagttctcgg gtgcgtatga gcagcgttgg aactcgacca
    14641 actccacgga gatttccggg ttccagatca tccagagcgg tacgccgatc acgacccgct
    14701 tcaccttcca cggtgtcgcc ggtcaaatcc tgaacggccg cggcgacctc ggtcgcactg
    14761 aggcgttcac tcagacagac ttgggtctcc gtcatcgcta ccgcttcggt aacgatggcc
    14821 ggttcgcctt cgtggcgaac gtggacatcc tgaacctgtt tgatgaagag aacgtgctgg
    14881 ggcacttcga ggccatctcc gcgacgggtg tcgatactga caacatcggg ttggcgacgg
    14941 gtaaccccgg cttcctcctc taccagacca ccaacacgag cacggcgttc aatcaggtgc
    15001 ttaatgctac gcgtgacccg cgttacaacc tgccgaactt tttccaaggt gcgcgcagtg
    15061 ttcgcttcgg gttcaaattg cagttctaaa gaactggttt agtcgttaga cttgaatagc
    15121 ccatccggcc aaaccggatg ggctattttt ttgccttgcg ggagaagcgt tcacatctcc
    15181 tcgccccgcc gtggcggccc tgacctatct catgggcttt gcctgttcta catcatcgag
    15241 ggggcaggct tcgccgccgg ctctcacaag acatcatcaa ctaatatcat taaggcgggc
    15301 cgcgacgcgc cggctcaggc atcgggt
//
LOCUS       DASVES010000109        31108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_921596, whole genome shotgun sequence.
ACCESSION   DASVES010000109 DASVES010000000
VERSION     DASVES010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 31108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31108
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921596"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VF240_03960"
     CDS             complement(<1..947)
                     /locus_tag="VF240_03960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7174422.1"
                     /translation="MNARLRSEGENVPPRVLHVVPALFGRGDGGVLGGAERYALELAR
                     HMAERVPTKLVTFGEREREETLGSLKVRVIGGAWHVRGQRTNPLAAALLAELRGADVV
                     HCHQRHVLASSLSAAVCRLTRRRVFVTDLGGGGWDVSAYVPTDRWYHGHLHISEYSRR
                     VYGHAGRSRAHVIHGGVDVEKFSPDPDASPREARAVFVGRLLPHKGIDDLIRALPDGV
                     ALDIIGQPYSEEYLRELKRLAEGRPVRFRHDCDDAELVRAYRGALCVVLPSVYRSAYG
                     QGHESKAPELLGQTLIEGMACAAPAVCTDVASMPEVVED"
     gene            complement(944..1525)
                     /locus_tag="VF240_03965"
     CDS             complement(944..1525)
                     /locus_tag="VF240_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7174423.1"
                     /translation="MFKVETRGLHAPAAPRSRVLNLGCGRKHVEGALNVDVTEATAPD
                     VVHDLNRRPWPFPDDHFEEVLAHDVVEHLDDVLATFEEIHRVCRDGATVRLSVPHFSS
                     GNAYTDPTHRHFFGYFTLDCFTEADERSFYTRARYRLRHRQIIFWPTLLNKIVWRLAN
                     RYPMWYELRWAWIFPALFLSMELEVVKGGEGRS"
     gene            complement(1540..2448)
                     /locus_tag="VF240_03970"
     CDS             complement(1540..2448)
                     /locus_tag="VF240_03970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7174424.1"
                     /translation="MPTVSVIILSYNRPALLRRALASVLAQSFDEVEVIVVDNASPAS
                     EEVARVVGERAGVKLIRNDANLGYAGGMNRGIECASGRYVYLTEDDIRLDPDCVRHLF
                     EHMEASTESGVCSPLMYNEAAGTIRCAGGALHLGGVYRRETFGEGESDTGQYARPFEV
                     TYVDGAVIFARAEFLRSLGGFREEYFMYVEAVEFSARVAKAGGKMFVVPGAKVYHFEP
                     AAGVGPHADLEFHKQKNLYSLYLLHAPARVLPEFFLRYGLLVPLRSMSAQRAAVWPLL
                     KAWGWLLLRAPSLVRERREAASVRAR"
     gene            complement(2511..3305)
                     /locus_tag="VF240_03975"
     CDS             complement(2511..3305)
                     /locus_tag="VF240_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7174425.1"
                     /translation="MTLAEWATYKLRAARTLGRMARWLSNWREVWSAYRAARPMPPLR
                     FRSGLVLHHGPDDSPVALLHEIFGEGHYTRHVGGRLEGVMIDLGANIGAVTVDWARRS
                     RNLRVHAYEPNPSTNGVLRRNVEANGLGACVTVHDEAVGRAAGELGLWTNINSMAVTG
                     YGESPPMPGAVRANVPLIDLNEVVARAGGGPVALLKMDIEGAEADALEGATPATLARI
                     TRVILEYHDVRCPGAARRCRTVLERAGFRCRVRRLNAENGLIYARR"
     gene            complement(3302..4144)
                     /locus_tag="VF240_03980"
     CDS             complement(3302..4144)
                     /locus_tag="VF240_03980"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX7174426.1"
                     /translation="MFILDAERLRSLAAGLSDAYASAEPFPHVVIDGLFPEDVLRRVA
                     NGFPRAGEELWHRFDNPRQRKLATLDETHIEANARWLLYQLNSSVFVNFLEELTGISG
                     LIPDPHFEGGGLHQIERGGFLKIHADFNRHPKLNLDRRLNLLLYLNENWREDYGGHLE
                     LWDKSVARCVERIAPVFNRCVVFTTTDFSFHGHPEPLFCPPGVTRKSVALYYYSNGRP
                     AEEATVAHGTLVRARPGETIDGLNGRTAGGLLKSMLRRLAPAGATGPASDAGKRGREG
                     GSDA"
     gene            complement(4231..5793)
                     /locus_tag="VF240_03985"
     CDS             complement(4231..5793)
                     /locus_tag="VF240_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX7174427.1"
                     /translation="MSRTRRFIGGLSFGYLNQFTVTVAGLWLTPFLLHRVGQSDYGLW
                     LVGTQLMAYLMLCDFGIVALLPRETAHATGRRLAAAGGEGVGAGEEDELPLLVGQTAR
                     LVLWQMPFVVSAAVLFWFLMPSEWEPLRRPFGFVLLAMVACFPLRVFQAVLQGLQDLE
                     FLAKANLGTWLLSTALTVALVIAGFGLYALAAGWVMAQVLATLVAVVRARRRFPEVLP
                     ARLPKLSWAEARTKLGSGFWASVSQVAVVLLVGTDMLVVGRVLGPEAVVVYACTYKLA
                     LVLANQPQLLLLSALPGLSELRAGASREHLSGVCAALNQAMLLLSGAVVCVVLAVNRG
                     FVAWWVGAEQYGGFLLTCLVLLNMLLRHWNLTMGIIMFSLGHEKRLALTGLLDGVVTV
                     GAGIILTLLIGPAGAPLGSVIGLCAVSLPGNMSALSGARAVRVPELLRSLAPWFWRFA
                     ALACACGLLAQRFVPATFPALAATVVVVACVYAALMLPVALRDPLGSYIRPRLPSFGK
                     RLFRAARLMGPA"
     gene            complement(5866..7125)
                     /locus_tag="VF240_03990"
     CDS             complement(5866..7125)
                     /locus_tag="VF240_03990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7174428.1"
                     /translation="MAEQAHMKGDDAAESRMRTSRESAPWVLVAGGFHARGGMDKANA
                     ALAAHLLERGTPLHLVAHSVAPELAGHALVTAHLVARPAGSYLLGEYLLSREGRRAAA
                     EVTAVRPDARVVVNGGNCIWPDINWVHYVHGAWSPGRPGAPVWFRVKDGAARAWALGR
                     ERRALLAARLVIANSESTREALVEAYGLKRDSVRVVYLGSDEGAAAVSAAERNAARAW
                     LSVAGRRPQVAFVGGLGLDQRKGLDTLLRAWRSLCARPEWDADLIIAGGGRGLAAVEK
                     QIAYEGLAGRVRLLGFTNRVAEVLAASDLLVSPARYEAYGLNVHEAVCRGVPAMVSAG
                     AGVAELYPAELSEMLLPDAEDAADLAARLLRWRAETEEWKSRFAPFAARLRRRAWGDA
                     AAEFVRVAEGDVHSAGAAGEALASCPA"
     gene            complement(7118..8494)
                     /locus_tag="VF240_03995"
     CDS             complement(7118..8494)
                     /locus_tag="VF240_03995"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7174429.1"
                     /translation="MISIVLCAAAFIFTYFAGRRSLVAGLVCVIGVGYFYGIMRANLQ
                     ETYSHFIFDAAVVGLYITQFSKKLPRGDSGRLAPLRLWVGILMGWPFLLFFIPVQDYA
                     VQAVGLRGNVFLLPFILLGARLGEQEVRQLAVAFAVLNLAVFVFAVAEYFLGVSRFYP
                     QNQVTVSIYKSLVDEDISKPLRSDLLRIPATFTGAHAYAGTMVLTFVYLFGAWARRGR
                     WVSWEKGLINAAMVATVLGIFMAAVRSPVIILALVLAVIFLTGHLKLHTLALFFAMVV
                     GVGWFVSSEERLQRFTSLKDTDLVTERVTWSVNENFLELMTEYPMGNGLGGGGTSIPY
                     FLQGRIVPPLFYMESEYARIVLEQGVFGLCLWLAFLVWAFARGTGSRDEPWFLGRRML
                     WVACAAFFATGMIGTGLLTSIPGTPLLLLSLGWIAVRQRGRAGASVPAAEHRGEGEWR
                     GLPAEQYG"
     gene            complement(8494..9255)
                     /locus_tag="VF240_04000"
     CDS             complement(8494..9255)
                     /locus_tag="VF240_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7174430.1"
                     /translation="MLNEARHLCGKNYLIEMRPGWFLRCHPTAYDFAYHLQNNDPDQI
                     AEFDGFISSCTPRMVLLDVGAHFGLFSLAALHFGGLEAKALAIDPSPIATRIMNIQLR
                     MNGVEGRGAVVQASASDQNGWQGLVATGVNGAGFFLPPQDHAGREITKTRATTLDALA
                     KEHNLKPTHIKIDVEGGEAEVLRGSEQLLSHVSPPLLLLELHNELIRSRCGNPSETLN
                     LLKKFGYQAYSFEMRPLSDDFILSQPLVRVLAKRQ"
     gene            complement(9377..10060)
                     /locus_tag="VF240_04005"
     CDS             complement(9377..10060)
                     /locus_tag="VF240_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7174431.1"
                     /translation="MTETVLTSAECAHEARARSSEGRSDHAIYAAVARALDERGAGGE
                     LLVDVGCGNGRLWPYVRARFARCVGVDLVRYEGLPHDVEFVRAELNGEALPLADGSAD
                     AVVSAETIEHLENPRAFMRELARVVRPGGWVVVTTPNQLSLLSLLTLVVKRRHAAFQD
                     VHYPAHLTALLEVDLRRIAAECGLHDVTVEYTGQGRVVLTPWHYPRFLARLAPRLCSD
                     NLLMIGRKH"
     gene            complement(10057..11187)
                     /locus_tag="VF240_04010"
     CDS             complement(10057..11187)
                     /locus_tag="VF240_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7174432.1"
                     /translation="MRVLHVHSGNLYGGVETLLLTLARRRELAPRIEPHYALCFDGRL
                     RDELREARAAVHALGGVRVSRPLSMRRARRALRELLSTYRFDAVVTHAPWSHAIFGPV
                     ARAEGLPLAFWMHGPASGGTHWLERWARLTPPDVVLCNSEYTAKDARRFFPRGRAELV
                     YCPVEAPTVQPTPSERAAVRAELDTPEGATVIVQASRMEPWKGHRTHLDALGLLKDVP
                     GWVCWFVGGGQRPREAAYLRELQGQAARLGIAERVRFAGQRSDVPRLLAAADIYCQPN
                     AEPEPFGIAFVEALYAGLPVVTTDEGGAVEIVDESCGTLVQPGDAGALAESLRALIEN
                     PLMRAALGRRAPARATELCEPATQMRRIEDILRGLARRREAA"
     gene            complement(11187..12500)
                     /locus_tag="VF240_04015"
     CDS             complement(11187..12500)
                     /locus_tag="VF240_04015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7174433.1"
                     /translation="MKIVYLNSLGEVGGAEGVLLDLLAAVREAEPGWSLRVIVPAAGP
                     LANRARALGVETEVVEFPPSVARLGEYAARGGAGRAALAARLLASSPQVARYVRRLRR
                     ALHRHAPDALHSNGLKMHALGSWAWGSSGAPAAVWHFHDYLGARPLMSRVLRASARSC
                     AAAVANSRSVAADVSDALGGRVSVRTVYNAVNLEKFSPVGPPADLDALAGVPSAAQGT
                     VRVGLVATLARWKGHETFLRALALVPRELNVRGYVVGGALYQTAGSQHSVEDLRQLAA
                     QLGVAERVGFTGHVEDAGHVMRALDVVVHASTEPEPFGLVIAEAMACGRAVLTSAAGG
                     AAEIVTPGVDALTHTPGDAEELARSIARLASDATLRDALGRAAHASARARFDRTRLAA
                     EWIPIYRGLKTANGGLAEALHSRGGESSSFISPQAEARGPQPAID"
     gene            complement(12822..14426)
                     /locus_tag="VF240_04020"
     CDS             complement(12822..14426)
                     /locus_tag="VF240_04020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX7174434.1"
                     /translation="MNTYIGLFVVSTFAAVVLTPLVRRACQRVGWLDLAADGRRIHLQ
                     PVPRLGGVAIFASVALSLLALTALDNLVTQQVREYLPRLAQTLVPAALVFAVGLYDDV
                     WGAGARVKFGALGVAATLFFFMGGRVEALSVPLVGTVELPEALSYVVTLVWMVGVANA
                     FNLIDGVDGLAAGAALFASLVMLVVALLLGHPPVAAVACVLCGALVGFLRYNFNPASI
                     FLGDCGALFVGFTLAGLSVHGAQKASTAVAVAIPVLAFGVPMVDTGFTLVRRFLSGRP
                     LFEGDREHIHHMLLARGWSQRRVAFVLYGACALFGLVALLFVHDSGGATGLMLLIVCA
                     AVALGVGRLRYHEVDEIKASVRRGLNDRRLRASHNIRVRRAVRAMSNAQSLGTFFDAV
                     RVLLEVGEFVYARVQLGDGPVGRRAAEALLARDAEGSRRHRLVIEDGLVCWSWERGDV
                     DAEVVSSSGRFWTLRLPLSTERGTWGYINLYRGFDSDELLLDINYLCHLFQRETGQAA
                     ERIFAAAIVEETGEDEIVSLAYSSGD"
     gene            complement(14575..15411)
                     /locus_tag="VF240_04025"
     CDS             complement(14575..15411)
                     /locus_tag="VF240_04025"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX7174435.1"
                     /translation="MIDLRCHLLEEGGRAGNTAAATAAGPDEALRMCHLAAGEGVSAI
                     VAPLKWPGGARRPPLDFEEYARRLDGLRRGLGDAGPRLVSGFVLELSEDLPALVEEYG
                     TRLAIGGGHYLLVSLPALEAPPSIESVWDRLGRLGFGVVVARPECCAALRQDSARLAR
                     WAAGGVILQLDAASITGAHGREAQRFARQLVRDHGSAGRIVVASNAHDTGASRPSLAT
                     AAASLRATYGRALARQLVERTPALVVGEGVGGRRGERAASLRRAGAGLLRMLRGGAVQ
                     QG"
     gene            complement(15456..17957)
                     /locus_tag="VF240_04030"
     CDS             complement(15456..17957)
                     /locus_tag="VF240_04030"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX7174436.1"
                     /translation="MSDKQEVSKPADRALELGGAAARAPLTLPAYAGYGPDADEGEGV
                     HLRDYWRAVRKRLWLVAGVVVCVTLLAAVYMARQPDVFVAQARVQVDLETVGASLGAA
                     KSQSVVINNPVNDPTYFNTQLQILTSPGLLRRVATTLDLEHNQSFRNPRSAAGRSSLR
                     GMLRALGIVSEGQQPQEVAGDDLPLTTSVAPAVSREDLAEARRLAPYVGALQGGLRVE
                     PVKETRTAGYVKDTRLIDISYRHADPQVAAKIVNAVADTFVLANLEKKTETNTSTGSF
                     LLRRVAELQSKIRTGEEQLANFARNHQILSLEGEQNIVVERLAGLNRQLLEAENERKL
                     AEAAFRAASEPKAADAMAETGSRHVGDAQAKLDELRQRRALLAEKYREEHPSMLEVNQ
                     LIVELERQLGANRDRAKDVGLTNLETRFRQAAAREQSLRAALGKQRDETLNQNDAAIN
                     YRILQQEIGTNKELLDGLLQRSRENDVVLAGTPNNISVIDYALAPGFPVGPKRKQGVA
                     LAFLLSTAFGVGLGLFLEYLNDTVRSSEDVERYLRLPALSVIPSAAFGVGGVSRLLPS
                     VGALQRRYGNGNRNGNGGGSGKGSLAVERPELLLNVDPRSALAESYRHLRTSVLLSRA
                     GQPPRTLLVSSSLPSEGKTTTSINTAISLAQTGARVVVVDADLRRPCLHRLFGAPNAL
                     GVSTILSREMSEEEMLELVHYDEVSKLHLLPSGPVAPNPAELLGSEQMRRLIGALATH
                     YTHVVIDSPPIVSFTDGVLISTMVDGVLLVVHGGKTSRTLLRRSKQILQDVGARVLGV
                     VLNNVSLQGDDYYYYRHYNSYYSPRAEAEAEGSAV"
     gene            complement(18520..23991)
                     /locus_tag="VF240_04035"
     CDS             complement(18520..23991)
                     /locus_tag="VF240_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX7174437.1"
                     /translation="MKSSARPPLAAAGLATRAPAKDGRVPGATLGVAVAALLFAVCSV
                     TYRHASARPAPFASTFIVNSTGDAPDSNLGDGLCSTGALVGVPPVIECTLRAAIQQAN
                     ADAIADTIAFDILVPAAGVRTISPASPLPAVTQPVTIDGYTQPGTLPNSLAASDNAVL
                     LIELNGTSAGAGADGLQIATTSTTLRGLIINRFGGDGVELSGAAGQNTVAGNFIGADS
                     AGTADLGNAGSGVRVSAGSTNTVGGSAPADRNVISGNDGDGVTIAGAGASGNSVLGNF
                     IGADKGGTADLGNTLSGVSIATSGNQIGDATTTPGQGAGNVISGNDAHGVAIVGAGTN
                     EVQGNLIGLQSGGAAARANALDGVNVTGAAAGNTIGGAAPGLRNIISSNGSDGVELNG
                     AGVTGTLVGANYIGASSGGTADLGNASNGVLIAGGATSNTVGGLTSSPGSSPGNIISG
                     NQSDGVEINNSNSNIVLGNLIGLQSNGLTALRNEGNGVFISSAASNTVGGYTATARNV
                     ISAHNASGTTDGVDIVNTASVNNKVIGNYIGTGVGGTETGLGNGGDGVRVAESADNNI
                     IGGDDDDDGTADAVVGARNVIGNNAGDGVEINGSGATGNVVAGNYIGIDKDGVGDIGN
                     TANGVLVSAATGNTIGGSTATPGTSAGNVISGNNGDGVELSGNADSNLVRGNLIGLRS
                     DGAGAVPNVANGVLLSGVISCTVGGATSDLRNVISGNLSDGVEIAGTASVNNVQGNYI
                     GTQTDGLSALANVGHGVLISANTNTVGGTVAGAGNTIAFNGGDGVYVSSGTANAIRRN
                     SIHSNIGLGIDLGADGVTLNDLDDPDTGANNLQNFPLIIDARTGSTVITGTLNSTPST
                     TFTIEFFYSAVPDPTAHGEGQNYLDSISVTTDAGGDAAFTHTATTTAPLGNVITATAT
                     NPAGNTSEFSRAHVVLAPTLSRLREFEATLVDGAGVALAWRTELEIDNLGFRLYREEA
                     GERTLITPSLVAGSALAVGPGTALTAGGSYTWTDAAGTPASRYWLEDVDADGVGTRHG
                     PFWPTSVSMQTRRQKKPRTSALLEQIGATQGGGSPQRGWAAQASVEGQSSSSGGGGVV
                     EGQSDSTGAGNVAASGITGPAAPEPDYMPADGVERQRVLAAGAAVKLSVRLPGWYRVT
                     RAQLEAAGLSPSADPAYLQLFAEGVEQAIHVNASAWAQNGVVEFYGEGLDTISADTRV
                     YWLIVGTTPGRRTSSAPQGGGKPRPPKGGHEPQPAASTAGANGGVKPPPSFDYTVERR
                     DRSVYYSALLNGEGSNFFGGVITPNAPAQALTVRGLRPADAVTARLEVRLQGVTAGPH
                     SVRVMLNGAEVGQLAFADRENKAAAFDVSPTLLLEGDNALGLTSAAPSDVSLTDYIRV
                     TYRRAYRAEADQLNFTAEGGAVTRVTGFTTANIRVVDITERGSVTEVVATVESVKIDE
                     GGGYAVRFTPGGTGRRTLLAFAGGAVSAVDGTRVNQPSQWYAASNAADFVIVTHADFR
                     AAATTLAERRVSEGLATTVVDVEDLYDEFSYGAHTPKAVRDFLVRAHSTWQRAPRYVL
                     LVGDGSYDPRNHLGRGLFDFVPARLIDTSQMETSSDEWLADFDGDGLAEMAVGRLPVR
                     TANEAALVVSKIVGHAPGTAERRLLLVSDRRGADGFDFEAATSSLLAHVPAGYETQRI
                     DRGARDAATVRAEIVGGVNSGPALVNYVGHGAVGVWTGEGLLRSSDALALSNGARLPL
                     FAMMTCLNGYYIDPSLDSLSESLLKAEHGGALAAWSSTGMTTPEAQAEMNRALYRALF
                     ESTGTLRLGDALRAARSSTANPDVRRTWVLLGDPTTKWR"
     gene            complement(24061..25152)
                     /locus_tag="VF240_04040"
     CDS             complement(24061..25152)
                     /locus_tag="VF240_04040"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7174438.1"
                     /translation="MIKTVAVPPQSTARLVASALAGAWRPSPPAFGGSANDLDAVAPL
                     LLGSGAGALGWWKIRHTELRDAPAAQELRQAYRLHTLQSALHERRIEQVFKLLGESGV
                     DAVLVKGLAASRLYPEAGLRPFGDIDVCVRPEQFAVAAAVLSGPRGQEFSVDLHRGFE
                     DAAGRGDDELFARAEVLRVGGVDVPVLCPEDHLRLLCVHLLRHGAWRPLWLCDVAAAV
                     EHRPEGFDWSRLLRGDGRRARWVTCAVGLAHELLGARIQDTPFERESRRLPRWLVPEV
                     MKSWETPYTHLQTPMRHRAPMTHYLRRPRGVLKDLANRWPNPIEATIRLGGPFNELPR
                     LPFQVGNVLVRTARFVSGLSAGARGERRS"
     gene            complement(25224..25706)
                     /locus_tag="VF240_04045"
     CDS             complement(25224..25706)
                     /locus_tag="VF240_04045"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX7174439.1"
                     /translation="MKDEESTAPQARTEGLIVREAEGEVLVYDPERHRAICLNQTAAL
                     VWRGCDGRTTVAGLARLLHDELDAPLAESAVWLALDRLGREHLLRERVRRPPGRAAIS
                     RRDLVRRIGVAAALPLITSILAPTAKAAASCLQSGMACTSPVECCSQLCPGAPNGACA
                     "
     gene            complement(25811..26317)
                     /locus_tag="VF240_04050"
     CDS             complement(25811..26317)
                     /locus_tag="VF240_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX7174440.1"
                     /translation="MEKKHRELKPLARKDGLLVRELPDEVLVYDLKGDKALCLNSTAA
                     LVWKRCDGRTTVGELTRLLGDDLGAAVDVDVVWYALGQLDKINLLEGREDTRAATTRT
                     IRRRDLLRRVGVAALTLPVVMAITAPSAQAQASLNCSGGTNRPAGCPCGSNNQCASNN
                     CLLSNICA"
     gene            complement(26435..27319)
                     /locus_tag="VF240_04055"
     CDS             complement(26435..27319)
                     /locus_tag="VF240_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174441.1"
                     /translation="MRKLDRLGWAESFTFESYGVRVGLRASRPGVLPALAEYLPPGWK
                     PVTAGVVERLYSLVAAPDAPPGGRVRRFSLLYEGILRIARTHELEELFERFAADLHLY
                     VAEAARRRVFVHAGVVGWRGRAIVLPGNSLSGKSTLVAELVRAGATYYSDEYAVLDRR
                     GRVHPFARPLGIRDTQSGRQRRVTVESLDGTSGSRPLPVGLVVVSKYRPGSRWRPRRL
                     SSGQGLLTLLSNTISVRRRPEAALDALQQVTAGAPVIKSTRGEARAAAEAILEAVESG
                     PVALGAKRFQPGPTTRPD"
     gene            complement(27640..28593)
                     /locus_tag="VF240_04060"
     CDS             complement(27640..28593)
                     /locus_tag="VF240_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX7174442.1"
                     /translation="MMKRAWRVLILCAALFVPQAQVLAWHETGHMLAAQIAYLRLSPA
                     AKAEVDRLLVAPADRRPLIHLCAGYYTRDTCEKIYDPVTIAVWMDDFRGDSLTDSYDN
                     WHYINYRPIFDGTPERANVGPLPINVLDRLNWCINTLRTGTGSDKRDAEVLGFLYHLV
                     GDVHQPLHAATRYSAKNPDGDAGGNFFTMQPPAGSKHKNLHSFWDNGAGLFGYDDLKR
                     PLDAAGRARIRQLADDLMKKYPPTGDDWKQTDPQAWVIESNQIARTFAYVKTKEGEAP
                     SAQYIEESRVIVGRRIALAGYRLAAVLDEILVRPPAPPRPR"
     gene            complement(28715..>31108)
                     /locus_tag="VF240_04065"
     CDS             complement(28715..>31108)
                     /locus_tag="VF240_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEX7174443.1"
                     /translation="AATAMSAARLAEIDAVVAQAIERKETPGAVVLAARKGRVMWRKA
                     YGARAVAPAREPMTADTIFDLASLTKVVATATSVMILVERGKIRLNDPVSRYIPELKG
                     EGRDRMTVEHLLTHRSGYAPDFDLKEQWTGHEEMLKRLYVEKLRSPPGARFVYSDIGL
                     IATAEVVQRVSGLPLDEFAKKNIYEPLGMRDTGFRPKRELLTRVAPTETVRGMRSYLG
                     GTGAAGADAERLLRGEVHDPTSNRMGGVAGHAGLFSTADDLAIFCQMILNGGEYNGVR
                     ILSPLGVEAMTRPRQVTEEGQARGIGWDITSSFSANRGDLFPTGSFGHTGFTGTSLWI
                     DPASETFVVFLSNRVHPDGKGDVGSLRSRVASIVASAVTDAGTVAHSRLQQSRYTQEM
                     LMNLVRFTSAAEGARAEASAATTAESPADAEVLTGIDVLARDGFKQLQGMRVGLVTNH
                     TGRDRRGRPTIDVLHAAAGVRLVALFAPEHGIRGLADEKVSDDVDEKTRLPIYSLYGE
                     SRRPRPEHLKDLDAVVFDIQDIGARFYTYISTLGHVLEEAAKAGKPVYVLDRPNPIGG
                     TEVEGPIADADKLSFVVYHTLPVRHGMTVGELAQLFNEQRKFGADLRVVKMENWRRAM
                     WLDSTGLTWVNPSPNMRSLTQATLYPGVGLLETTNVSVGRGTDTPFEVLGAPWIDGQR
                     LAAHMNARRLPGIRFVPVRFTPKASVYKGEECGGVNFIVTDRAAFRPVRVGIELAVAL
                     RRFHPAEWKVDDMLRLLANADTLARVRSGASAEEVMRSWQAGLDEFKNARAKFLIYR"
BASE COUNT         5206 a        10541 c        10444 g         4917 t
ORIGIN      
        1 ctgtcttcga ccacttccgg catactcgcc acgtccgtac acacggccgg cgcagcgcac
       61 gccatcccct cgatgagcgt ctgccccagc agctccggcg ccttcgactc gtgcccttgc
      121 ccgtaggcgc tccggtagac gctcggcagc accacgcaca acgcccctcg ataagcgcgc
      181 accagctccg cgtcgtcgca gtcgtgccgg aacctgaccg gcctgccctc ggccagccgc
      241 ttcagctctc gcaggtattc ctccgagtag ggctgaccga tgatgtcgag cgccacgccg
      301 tccggcaaag cgcggatgag gtcgtcgatg cccttgtgcg gcagcagccg tccgacgaag
      361 accgcccgcg cctcgcgtgg cgacgcgtcg gggtcgggcg aaaacttctc cacatcgacg
      421 cctccgtgaa tgacgtgggc gcgcgagcgc cccgcgtggc cgtagacgcg gcggctgtat
      481 tcgctgatgt gcaggtggcc gtgataccag cggtcggtcg ggacgtaggc cgacacgtcc
      541 cacccgccgc cgccgaggtc tgtgacaaaa acgcgtcgcc gcgtgagccg gcacacggcc
      601 gcgctcaagc tgctcgccag cacgtgccgc tgatgacagt gtacgacatc cgccccgcgc
      661 agctccgcga gcagcgccgc cgccagcggg ttcgtccgct gcccgcgcac gtgccacgcg
      721 ccgccgatga cgcgtacctt caagctcccc agcgtctcct ctcgctcgcg ctcgccgaag
      781 gtgacgagct tcgtcggcac acgctcggcc atgtgacgcg ccagctccag cgcgtaacgc
      841 tcggccccgc ccaacacccc gccgtcgccg cgcccgaaca gcgcggggac gacgtgcagc
      901 acacgcggcg gcacgttctc accctcactt ctcagccgtg cgttcaactg cgaccttctc
      961 ctcccttgac gacctcaagc tccatgctca ggaacagcgc cgggaaaatc catgcccagc
     1021 gcagctcgta ccacatcggg tagcgattcg cgagccgcca cacgattttg ttcagaagcg
     1081 tcggccagaa gatgatctgc cggtggcgca aacggtatcg ggcgcgcgtg tagaacgaac
     1141 gctcgtcggc ttcggtgaag cagtcgagcg tgaagtagcc gaagaagtgc cggtgcgtcg
     1201 ggtcggtgta ggcgttgccc gatgagaagt gcggcaccga caggcggacg gttgcgccgt
     1261 cgcggcacac gcgatgaatc tcctcgaagg tcgccaacac gtcgtcgagg tgctcgacca
     1321 cgtcgtgcgc cagcacctcc tcgaagtggt cgtcggggaa gggccacggc cgtcgattga
     1381 ggtcgtgaac cacgtccggc gcggtcgcct ccgtcacgtc cacgttcaac gcgccctcga
     1441 cgtgcttgcg cccgcacccc aaattcagca cccggctccg tggcgcagcc ggcgcgtgta
     1501 atcctcttgt ctcgaccttg aacatttcag ccacagtgac taacgagcgc gcaccgacgc
     1561 cgcctcacgc ctctctctga cgagcgacgg cgccctgaga agcagccagc cccacgcttt
     1621 gaggagcggc cagacggccg cgcgttgggc cgacatcgag cgcaaaggga cgagcagccc
     1681 gtagcgcagg aagaactccg gcaacacgcg cgcgggggcg tggaggaggt agagcgagta
     1741 gaggttcttc tgtttgtgaa actccaaatc cgcgtggggg ccgaccccgg ccgcaggctc
     1801 gaaatggtag accttggccc ccggcacgac gaacatcttc cccccggcct tcgccacgcg
     1861 cgccgaaaac tccacggcct cgacgtacat aaagtactcc tcgcggaagc cgccgagact
     1921 tctcaagaat tccgcgcggg cgaagatgac cgcgccgtcc acgtatgtaa cctcgaaggg
     1981 ccgcgcatac tgccccgtgt cgctctctcc ctcgccgaaa gtctcccggc gatagacgcc
     2041 cccgaggtgg agcgcgcccc cggcgcagcg aatcgtcccc gccgcctcgt tgtacatcag
     2101 gggtgagcag acgccggact ctgtgcttgc ctccatgtgc tcgaacaggt gccggacgca
     2161 gtcggggtct aaacggatgt cgtcctccgt caaataaacg tagcgccccg acgcgcactc
     2221 gatgccgcgg ttcatcccgc ccgcgtagcc gaggttcgcg tcgttgcgga tcaacttcac
     2281 gcccgcccgc tcgccgacga cgcgcgccac ctcctcgctc gccggactcg cgttatcgac
     2341 gacgatcact tccacttcat cgaacgactg cgcgagcaca gatgccaacg cccgccgcag
     2401 caacgccggg cggttgtagc tcaggataat gacggagact gtcggcatct tcggtgtcgt
     2461 gtcggcgacg cgcgcgcccc cagcgtcgcg accgcccgcg cgctcgctac ttaacggcgt
     2521 gcgtagatca atccgttctc cgcgttgagt cgccgcacgc ggcagcggaa gccggcgcgc
     2581 tccagaaccg tgcggcaacg ccgggcggcg ccggggcagc gcacgtcgtg gtattcgaga
     2641 atgacgcgcg tgatgcgggc gagcgtggcg ggcgtggcgc cttcgagcgc gtcggcctcg
     2701 gcgccctcga tgtccatctt gagcagcgcc acggggccgc cgcccgcgcg cgcgaccacc
     2761 tcattcaagt cgatgagcgg gacgtttgcg cgcaccgcgc ccggcatcgg cggcgactcg
     2821 ccgtagccgg tcacggccat gctgttgatg ttcgtccaca gtccgagttc gccggccgcg
     2881 cggccaacgg cctcgtcgtg gacggtcacg cacgcgccga ggccattggc ctcgacgttg
     2941 cgccgcagaa ctccgttggt cgaagggttc ggctcgtagg catgcacgcg caagttccgc
     3001 gagcgccgcg cccaatcaac cgtcacggcc ccgatgttgg cgcccaaatc gatcatcacg
     3061 ccttccagcc ggcccccgac gtggcgcgtg taatggccct cgccgaaaat ctcgtggagg
     3121 agcgcgacgg ggctgtcgtc ggggccgtgg tggagcacca gacctgagcg gaagcggagc
     3181 ggcggcatcg ggcgcgctgc tcgataggcc gaccagacct cgcgccagtt cgatagccag
     3241 cgggccatcc ggccgagcgt gcgcgccgcg cgaagtttgt atgtcgccca ctccgcgagg
     3301 gtcatgcgtc agagcctcct tccctgccgc gcttccccgc gtccgacgcc gggccggtgg
     3361 cgccggcagg cgcgagccgc cgcagcattg acttcaggag gccgccggcg gttcgaccgt
     3421 tcagcccgtc gatggtctcg ccgggacgcg ccctgacgag tgttccgtgt gcgacggttg
     3481 cctcctcagc gggccgaccg ttgctgtagt agtagagggc caccgacttg cgcgtcacgc
     3541 cgggcgggca gaagagcggt tcggggtgcc cgtggaagga gaagtcggtc gtcgtgaaga
     3601 cgacgcaacg gttgaagacc ggcgcgattc gttcgacgca acgcgcgacg ctcttgtccc
     3661 agagttcgag gtggccgccg tagtcttctc tccagttctc gttgagatag aggagcaggt
     3721 tgagccggcg gtcgagattg agcttcgggt gacggttgaa gtcggcgtgg attttgagaa
     3781 agccgccgcg ctcgatctgg tgcagcccgc cgccctcgaa atgcgggtcg gggatgagac
     3841 cgctgatgcc ggtaagctct tccagaaagt tgacgaagac ggaggagttg agctgataca
     3901 aaagccagcg ggcgttcgct tcgatgtgcg tctcgtcgag agtcgccagc ttcctctgtc
     3961 ggggattgtc gaagcggtgc cagagttctt ctccggcgcg tgggaaacca ttcgccaccc
     4021 gccgcagaac gtcttcgggg aacaggccgt cgatgaccac atgcgggaac ggctcggcgc
     4081 tcgcatacgc gtcagacagc cccgcggcca gcgaccgaag tcgctccgcg tcaaggatga
     4141 acatctccaa aggattcata aaaagttagt gaacgcgcac cgtcgtcatc ggccacacgc
     4201 gcacgagccg cgtccgtctg tcgcacgccc tcacgccggc cccatcaagc gcgccgcccg
     4261 gaaaagtctc ttgccgaacg aaggcagacg cgggcggatg taactcccga gcgggtcgcg
     4321 cagcgcgacc ggcagcatca gcgccgcgta gacgcacgcc acgaccacca ctgtggcggc
     4381 cagcgccgga aaggtcgcgg gcacgaagcg ttgggcgaga aggccgcacg cgcaggcgag
     4441 cgcggcgaag cgccagaacc acggcgcgag cgaccttagt aattcaggga cgcgcacggc
     4501 tcgcgccccc gaaagcgccg acatgtttcc gggcagactc accgcgcata gcccgatcac
     4561 ggagccgagc ggagcgccgg cggggccaat gagcaaggtg aggatgatgc cggccccgac
     4621 ggtcacgacg ccgtcgagca accccgtcag ggcgaggcgc ttttcatggc cgagcgagaa
     4681 catgatgata cccatggtca ggttccagtg gcgcagcagc atgttgagga gcacgaggca
     4741 cgtcagcaaa aagccgccat actgctccgc gcccacccac cacgcgacga acccgcggtt
     4801 gaccgccagc accacacaca cgaccgcccc gctcaaaagc agcatcgcct gattcagcgc
     4861 cgcgcacacg cccgacaagt gctcgcgcga cgctcccgcg cgcagctcgc tcaaacccgg
     4921 cagcgccgac aacaacaaca actgcggctg gttcgccagc accagcgcca gcttgtacgt
     4981 acacgcgtag acgacgaccg cctccggccc caacactctt cccacgacga gcatgtccgt
     5041 gcccacgagc agcacgacgg cgacctgact cacgctggcc cagaagcccg accccaactt
     5101 cgtgcgcgcc tccgcccacg aaagcttagg caagcgcgcg ggcaacacct cggggaagcg
     5161 ccggcgcgcg cgcacgacgg cgacgagggt cgcgagcacc tgcgccatca cccagccggc
     5221 cgccagcgcg tagaggccga agccggcgat gacgagcgcc accgtcagcg ccgtgctcag
     5281 gagccacgtt ccgaggttcg ccttcgccag aaattccaaa tcctgcaatc cctgcaacac
     5341 ggcctgaaag acgcgcagag ggaagcacgc gaccatcgcc agcagcacga agccgaacgg
     5401 gcggcgcaga ggctcccact ccgacggcat caggaaccag aaaagcacgg ccgcgctcac
     5461 cacgaagggc atctgccaga gcacgagtcg cgccgtctgc ccgacgagca acgggagttc
     5521 atcttcttcg cccgcgccca cgccttcgcc gcccgccgcg gcgaggcgtc gccccgtggc
     5581 gtgcgccgtc tcgcgcggca gcagcgcgac gataccgaag tcgcacagca tcagataggc
     5641 catgagctgc gtgccgacga gccacaggcc gtagtcgctc tgcccgacgc ggtgcagcaa
     5701 aaacggagtc agccacagcc ccgccaccgt caccgtgaac tggttgaggt agccgaagct
     5761 gagaccgccg atgaaacgcc gcgtccgact catagcaacc gcgccaactc ctttaaccct
     5821 ccgcccaaaa tcgaaaacaa gcagacgccg ggcagtagac gaacgctacg ccgggcagga
     5881 agccaatgcc tcgccagccg cgcccgcgct atgcacgtcg ccctcggcca cacggacaaa
     5941 ctcggccgcc gcgtcgcccc acgcacggcg ccttagtctc gccgcgaagg gcgcgaaacg
     6001 cgacttccac tcttccgtct ccgcgcgcca gcgcaggagt ctcgccgcga ggtcggcggc
     6061 gtcctccgcg tcgggcagga gcatttccga cagctcagcc gggtagagtt cggcgactcc
     6121 agcgcccgcg ctcaccatcg ccggcacgcc ccggcagacg gcctcgtgga cgttcaaccc
     6181 gtatgcctcg tagcgagcgg gactgaccag aaggtcggag gccgccagaa cttcagccac
     6241 gcgattcgtg aaaccgagca ggcggacgcg cccggcgaga ccctcatagg cgatctgctt
     6301 ctcgactgcg gcaagccctc ggcctccgcc cgcgatgatc aaatccgcgt cccactcggg
     6361 gcgggcgcac aaacttctcc acgcgcgcag caaggtgtcc agaccttttc tctgatcgag
     6421 gcccagcccg ccgacgaagg cgacctgcgg ccgtctcccc gcgaccgaca gccacgcgcg
     6481 cgccgcgttg cgctcggcgg cggaaacggc ggcagcaccc tcgtccgagc ctaagtacac
     6541 gacgcgcacg ctgtcacgct tcagcccgta tgcttcaacc agagcttctc gagtggactc
     6601 cgaattcgcg atgacgagcc gggccgcgag tagtgcgcga cgctcccggc cgagcgccca
     6661 cgcgcgcgcc gccccgtcct tcaccctgaa ccacacgggc gctccgggcc ggcccggcga
     6721 ccacgcgccg tgaacgtaat gcacccagtt gatatcgggc cagatacagt ttccgccatt
     6781 cacaacaacg cgcgcgtccg gccgcacggc cgtcacttcc gcggcggccc gccgcccctc
     6841 ccgcgaaagc aagtactcgc cgagaaggta cgacccggcg ggccgcgcga cgaggtgcgc
     6901 tgtgaccagc gcgtggccgg caagctccgg cgcaacgctg tgggcgacga ggtgcagggg
     6961 cgtgccgcgc tctaggaggt gcgcggccag cgcggcgttg gccttgtcca tgccgccgcg
     7021 cgcgtgaaat ccgccggcga cgagcaccca cggcgcgctc tcccggctcg ttcgcatgcg
     7081 actctccgcc gcgtcgtctc ccttcatgtg cgcttgctca gccatactgc tctgccggca
     7141 gccccctcca ttcgccctcg ccccgatgct ccgccgccgg cacgctcgcg ccggcgcgcc
     7201 cgcgctggcg caccgcgatc cagccgaggc tcaggagcag gaggggtgtg cccggaatcg
     7261 aggtcagcag cccggtgccg atcatccccg ttgcgaagaa ggccgcgcac gcgacccaca
     7321 gcatccgccg tcccaaaaac cacggctcat cgcgcgaccc cgtcccgcgg gcgaaggccc
     7381 acaccaggaa ggccaaccac agacacaggc cgaacacgcc ctgttcgagc acgatccgcg
     7441 cgtactcgct ctccatgtag aagagcggcg ggacgatccg gccctgtaaa aagtagggga
     7501 tgctcgtccc acccccgccg aggccgttgc ccattgggta ctcggtcatc agctccagga
     7561 agttttcgtt gacactccag gtgacgcgct ccgtcacgag gtccgtgtct ttcagcgaag
     7621 tgaagcgttg caggcgctcc tcgctggaga cgaaccagcc cacgccgacg accatcgcaa
     7681 agaacagcgc cagcgtgtgc agcttcaggt gccctgtcag gaagatgacg gcgaggacga
     7741 gcgccagaat aatgactggg ctgcgcaccg ccgccatgaa gatgcccagc accgtcgcca
     7801 ccatcgccgc gttaatcagg cccttctccc acgacaccca acggccccgg cgcgcccatg
     7861 cgccgaacag gtagacgaat gtcagaacca tcgtgcccgc gtaggcgtgc gcccccgtga
     7921 acgtcgccgg gatgcgcagc aagtcgctcc tcagcggttt gctgatgtct tcgtcaacga
     7981 ggcttttgta gatgcttacg gtgacctgat tctgcgggta gaagcgtgag acgccgagaa
     8041 agtactccgc caccgcgaag acgaagacgg cgaggttgag cacggcgaag gccaccgcga
     8101 gttgacgcac ttcctgttcg cccagccgcg ccccgaggag aatgaagggg agaagaaaga
     8161 cgttgccgcg caggcccacc gcctgaacgg cgtaatcctg cacggggata aagaagagca
     8221 ggaacggcca ccccatcagg attcccaccc atagccgcaa tggcgccagc ctcccgctgt
     8281 ctccacgcgg cagctttttc gagaactgtg tgatgtaaag accgacgacc gccgcgtcga
     8341 agatgaagtg ggagtaagtc tcctgcaaat tggcccgcat gatgccatag aaataaccga
     8401 ccccaatgac gcacactaac ccggcgacga gcgagcggcg cccggcgaag taagtgaaga
     8461 taaaggcggc ggcgcaaagg acgattgaaa tcattactgc ctcttcgcga gcacccgaac
     8521 taatggctgg ctcaatatga aatcatcact gaggggccgc atctcaaagg agtaagcctg
     8581 atacccgaat ttcttgagca gattaagggt ttccgatgga ttgccacacc gactgcgaat
     8641 gagttcgtta tgaagctcca gcaacaatag cggtgggctg acatgagaca gcaattgttc
     8701 actcccgcgc agcacttccg cctcgccccc ctccacgtca atttttatgt gcgtcggttt
     8761 aaggttatgt tccttcgcca gcgcgtctaa agtcgtcgcc cttgtctttg taatttcccg
     8821 cccggcatga tcttgaggag gcaggaaaaa ccctgcgcca ttcacacctg tcgcaaccaa
     8881 tccctgccac ccattctgat cgctcgccga tgcctgaacc acagctcccc gcccttccac
     8941 accattcatt cgcagttgaa tgttcattat tcgcgtggct atcggggaag ggtctatagc
     9001 gagcgccttc gcttcaagcc ccccaaaatg taacgccgcg aggctaaaca gcccgaaatg
     9061 tgcccccaca tccagcagca ccattcgggg cgtacaactt gagataaagc cgtcgaattc
     9121 cgcaatctga tccgggtcgt tgttctgcaa atgataagca aagtcgtagg cggtcggatg
     9181 gcaacgcaag aaccatccag gacgcatttc gattaagtag tttttcccgc acaggtgccg
     9241 cgcctcattc aacaaccacc gcgctgatct tcttggtgtg cgcagccaat tccgaagtgg
     9301 acggggcgct aaatcccgag cgattgcttt gaagagaaat ctcattactt agagcttgaa
     9361 tctccacaca tcccaatcaa tgcttcctgc cgatcatcag taaattatca gagcacaatc
     9421 tgggcgcgag ccgcgcgagg aacctcgggt aatgccacgg cgttaacacc acacgtcctt
     9481 gccctgtgta ctcgacggtc acatcatgca gcccgcactc ggcggcgata cgtcgcagat
     9541 cgacttcgag gagcgccgtc aggtgcgccg ggtagtgtac gtcctgaaag gccgcgtgcc
     9601 ggcgcttgac gacgagcgtc agcagactca gcaggctcaa ctggttcggc gtcgtgacga
     9661 cgacccagcc gccgggccgg acgacgcgcg ccagctcgcg catgaaggcg cgcgggttct
     9721 ccagatgctc tatcgtctca gccgacacca ccgcgtcggc gctcccatcg gccagcggca
     9781 acgcctcgcc gttcaactcg gcgcgcacga attcgacatc atgaggcagc ccttcgtagc
     9841 gcacgaggtc aacgcccaca cagcgcgcga agcgagcgcg cacgtatggc cagaggcgcc
     9901 cgttgccgca accgacatcg accagcaact cccctcccgc gccccgctca tcgagtgcgc
     9961 gcgcgacggc cgcgtagatg gcgtggtcgc ttcgcccctc gctgctgcgc gcccgcgcct
    10021 cgtgcgcgca ctcggccgac gtgagcaccg tctccgtcat gcggcctccc ttcggcgcgc
    10081 cagcccgcgg agaatgtctt cgatccgcct catctgcgtc gccggctcgc aaagctccgt
    10141 ggcccgcgcc ggcgcgcgcc ggccgagcgc ggcgcgcatc aacggattct caatgagcgc
    10201 gcgcagcgac tcggcaagcg cgcccgcgtc gcccggctgc accagcgttc cgcacgactc
    10261 atcaacgatc tcgacggcgc cgccctcatc ggtcgtcacc acaggcaggc ccgcgtacag
    10321 cgcctcgacg aaggcgatgc cgaacggctc cggctcggcg ttcggctggc agtagatgtc
    10381 ggccgcggcg agcaggcgcg gcacgtcgga gcgttggccg gcgaagcgga cgcgctccgc
    10441 gatgccgagc cgcgccgcct gcccctgcaa ctcgcgcagg tatgcggcct cacgcgggcg
    10501 ctgcccgccg cccacgaacc agcagaccca gcccggcacg tccttcaaaa gcccgagcgc
    10561 gtcgaggtgc gtgcgatgcc ccttccacgg ctccatgcgg ctcgcctgaa cgatgacggt
    10621 cgcgccctcg ggcgtgtcga gttcagcacg gacggccgca cgctcggacg gcgttggctg
    10681 cacagtggga gcctcgaccg gacagtagac gagttccgcg cgcccgcgtg ggaagaagcg
    10741 gcgcgcgtct ttcgccgtgt actcgctgtt gcaaagtacg acatcggggg gcgtcagacg
    10801 cgcccagcgt tccagccagt gtgtgccgcc gctggccggg ccgtgcatcc agaacgcgag
    10861 tggcaacccc tcggcgcgcg cgaccgggcc gaagatggcg tgcgaccacg gggcgtgtgt
    10921 gaccacggcg tcgaaacgat aggtcgagag cagctcacgc agcgcgcggc gcgcacgcct
    10981 catgctcagg ggccggctca cgcgcacccc gccgagggcg tgcacggcgg cgcgcgcctc
    11041 gcgcagctcg tcgcgcaggc gaccgtcgaa gcagagcgcg tagtgcggct caatccgtgg
    11101 ggccagttcc cgccggcgcg cgagcgtcag cagcagggtc tccaccccgc cgtacaggtt
    11161 gccgctgtgg acgtgtagaa cgcgcatcag tcgattgcag gctgcgggcc gcgggcttcc
    11221 gcctgcgggc taatgaaaga cgaactctcg ccgccccgtg aatgtagggc ttcggcgagt
    11281 ccgccgttcg cggtcttcag tccgcgataa atgggaatcc actcggcggc gaggcgcgtg
    11341 cggtcgaagc gcgcgcgggc gctcgcatgg gcggcgcgtc cgagcgcgtc gcgaagagtg
    11401 gcgtcggacg caagacgcgc gatagagcgg gcgagttctt cggcgtcgcc gggcgtgtgc
    11461 gtgagtgcgt ccacgccggg cgtgacgatc tccgcggcgc cgccggccgc actcgtcaac
    11521 acggcgcgac cgcaggccat cgcctcggcg atgaccagtc cgaacggctc cggctcggtg
    11581 ctggcatgca caaccacgtc gagcgcgcgc atcacatggc ccgcgtcttc cacgtggccg
    11641 gtgaagccga cgcgctcagc cacgcccagt tgtgcggcaa gttggcgtag gtcttcgacg
    11701 ctgtgctggc tacccgccgt ctggtagagc gcgccgccca cgacgtagcc cctcacgttc
    11761 aactcacgcg gcacgagggc gagcgcccgc aaaaatgtct cgtgtccctt ccagcgggcg
    11821 agcgtggcga cgaggccgac cctcactgtg ccttgtgcgg ccgatggcac accagcgagc
    11881 gcatccagat cggccggcgg ccccacggga gaaaacttct caaggttgac ggcgttgtaa
    11941 acagtccgca cgctgacgcg cccgccgagc gcgtcgctca cgtcagccgc gacactgcgg
    12001 gagttcgcca cggccgcggc gcatgagcgc gccgacgctc gcagcacgcg cgacatgaga
    12061 ggccgcgcgc ccagatagtc gtggaagtgc cacaccgctg ccggtgcgcc ggatgagccc
    12121 cacgcccacg agccgagcgc gtgcatcttg agtccgttcg agtgcagagc gtcgggcgcg
    12181 tgtctatgga gtgcgcggcg cagacgccgc acgtagcgcg cgacctgcgg actcgacgca
    12241 agcaggcggg ccgcgagcgc cgcgcgccct gcgccgccac gggccgcata ctcgccgagc
    12301 ctcgcgaccg acggcggaaa ctccacgacc tccgtctcca caccaagggc gcgcgcacgg
    12361 tttgccagcg gccccgcggc cgggacgatg acacgcaacg accatcccgg ctcggcctca
    12421 cgcaccgccg ccaagaggtc gagcaggacg ccctccgcgc cgccgacctc cccgagcgaa
    12481 ttgagataaa cgatcttcat ctttaactat tcgccgacaa cttcaattcc ttacccgcgc
    12541 cggctttgtg tccgtagcaa gaccaaccgg cgttcgtcac atctgtaggg gcgggggcaa
    12601 gtccgcggcc cgcaaggtgc ggaaggaatt aacatggatg gacaggatgg gtaggatgaa
    12661 aaaagacctg atttgagatt tgagatttca gatttgagat ttcacatcat ctcatcctgt
    12721 ctgtcctgca aatcctgtta atcctgtcca tccttgttaa ttgttccttc gtaaagacgg
    12781 gccgcggact tgccctgccc ctacggtttt gccatgcact cttaatcgcc cgagctgtac
    12841 gcgagcgaca cgatttcatc ctcgcccgtc tcttccacaa tcgccgcggc gaagatcctc
    12901 tcggccgcct gacccgtctc gcgttggaag agatgacaga gatagttgat gtcgaggagg
    12961 agttcgtcgg agtcgaagcc gcgataaagg ttgatgtagc cccaggtgcc gcgctcggtc
    13021 gagagcggca gacgcagcgt ccagaagcgg cccgaggagc taaccacctc ggcgtccacg
    13081 tcgccgcgct cccaactcca gcaaacgagt ccgtcttcaa tcacgagccg gtgccgccgc
    13141 gagccttcgg cgtcgcgcgc gagcagcgcc tcggccgccc gtcgtccaac agggccgtcg
    13201 ccgagctgca cgcgcgcgta gacgaactcg cccacttcga gcaacacgcg cacggcgtcg
    13261 aagaacgtgc cgaggctctg cgcgttcgac atggcgcgca ccgcacggcg cacgcggatg
    13321 ttgtgcgagg ctcgcagccg ccggtcgttc aagccgcgcc ggacgctcgc cttgatctcg
    13381 tccacctcgt gataacgcag ccggcccacg ccgagcgcca cggccgcgca gacgatcaac
    13441 agcatcagcc ccgtggcccc gccagaatcg tgtacgaaga ggagagccac gagtccgaag
    13501 agcgcgcacg cgccgtagag gacgaaggcc acgcggcgct gcgaccagcc gcgggcgagg
    13561 agcatgtggt ggatgtgttc gcggtcgccc tcgaacagcg gacgcccgct caggaagcgg
    13621 cggacgagtg tgaacccggt gtccaccatg gggacgccga aggcgagcac ggggatggcc
    13681 acggccacgg ccgtcgaagc cttctgcgcg ccgtgcacac tcaggccggc gagtgtgaag
    13741 ccgacgaaga gcgcgccgca gtcgccgaga aagatcgacg ccgggttgaa gttgtaacgc
    13801 aggaatccga cgagcgcgcc gcacagcacg caggccacgg ccgccaccgg cgggtgcccc
    13861 agaagcagcg ccaccaccag catcaccagc gacgcaaaca gcgccgcccc ggccgccagc
    13921 ccgtccacgc cgtcaatcaa attgaaggcg ttcgctacgc ccaccatcca caccagcgtc
    13981 acgacatacg acagagcttc gggcagctcg accgtgccca cgagcggcac cgacagagcc
    14041 tcgacgcgcc cgcccatgaa gaagaaaagc gtggcggcca cgccgagcgc cccgaacttg
    14101 acgcgtgcgc cggcgcccca cacgtcgtcg tagagtccga cggcgaagac gagggccgcc
    14161 ggcacgagcg tctgcgcgag ccgcggcagg tattcgcgca cctgctgcgt gacgaggttg
    14221 tcgagggcgg tcaaagcgag cagcgagagg gcgacggacg cgaagatagc cacgccgccg
    14281 agacgcggca cgggctggag gtgtatgcgc cggccgtcgg ccgcgaggtc gagccacccc
    14341 acccgctggc aggcgcgccg caccagcggc gtcagcacga cggccgcgaa tgtcgagacg
    14401 acgaacagac cgatgtatgt gttcatctgt gctgcctcaa caaagctatt agcttttagc
    14461 ggttagcttt cagtaagaaa caagagtcga agcgcaaagc cgaagcgcgt ccgcgttgat
    14521 taataaatct ctcgttcgcc atcttccagc taacagctaa aagctaatag cttttcaccc
    14581 ctgctgcacg gcccctccgc gcagcattcg cagaagcccg gccccggcgc gacgcaggct
    14641 cgccgcgcgc tcgccgcgcc gcccgccgac gccctcgccg acgacgagcg ccggggtgcg
    14701 ttcgacgagt tgccgcgcca gcgcacgccc gtatgtcgcc cggagcgacg cggcggcagt
    14761 ggcgagcgac ggccggctcg ctcccgtgtc gtgcgcgttc gaggccacga cgatgcgccc
    14821 cgcacttccg tgatcgcgga cgagctggcg tgcgaagcgt tgcgcctcgc ggccgtgcgc
    14881 gcccgtgatg ctcgccgcgt cgagttgcag gatgacgcct cccgcggccc agcgcgcgag
    14941 tcgcgcggag tcttgccgca acgccgcgca gcactcgggc cgcgcaacga caacgccgaa
    15001 gccgagacgc ccgagcctgt cccacaccga ctcgattgac ggcggagctt cgagcgcggg
    15061 caaactgacg aggaggtaat gaccaccgcc gatggcgagc cgcgtcccat attcctcgac
    15121 gagcgccggc aagtcctcgg agagttcgag cacgaagccg gacactagtc ggggccccgc
    15181 gtcgcccagc ccccggcgca ggccgtccag ccgccgcgcg tactcctcga agtcgagcgg
    15241 cggacggcgc gcgccccccg gccacttcag tggtgcgacg atggcgctca cgccctcgcc
    15301 cgcagccaga tggcacatcc gcaaggcttc gtccggccca gcggcggtag cagcggccgt
    15361 gttgcccgcg cgcccgcctt cttccagtag atgacagcgg agatcgatca tggtttctcg
    15421 gataccccca caacgctcgc tcggttcgtg atgcctcaga cggcgctccc ctcggcctcc
    15481 gcctccgcac gcggcgaata gtaggagtta tagtgccggt aatagtaata gtcgtcgcct
    15541 tgcaggctaa cgttgttgag gacgacgccg aggacgcgcg cgcccacgtc ctgcaaaatc
    15601 tgtttggagc gacgcagcag agtgcgcgag gtcttgccgc cgtggacgac gagcaggaca
    15661 ccgtccacca tcgtcgagat gagcacgccg tcggtgaaag agacgatggg cggcgagtcg
    15721 atgacgacgt gcgtgtagtg tgtggcgagg gcgccgatga ggcgacgcat ctgctcggag
    15781 ccgaggagtt cggccgggtt gggcgcgacc ggccccgagg gcagcaggtg gagcttgctc
    15841 acctcgtcgt aatgcaccag ctccagcatc tcctcctcgc tcatttcccg tgagaggatc
    15901 gtgctcacgc cgagcgcgtt gggcgcgccg aagaggcggt gcaggcatgg gcggcgcagg
    15961 tcggcgtcca cgacgacgac gcgcgcgccc gtctgcgcga gactgatggc cgtattgatg
    16021 ctcgtcgtcg tcttgccttc gctcggcagg gacgaggaga cgaggagcgt gcgcggcggc
    16081 tggccggcgc gcgaaagcag cacggacgtg cgcaggtggc ggtaggactc ggcgagggcc
    16141 gagcgcggat cgacgttgag caatagctcc ggccgctcga cggccaagga gcctttcccg
    16201 ctgccgccgc cgtttccgtt tctgtttccg ttgccgtaac gtctttgcag cgcgcccacg
    16261 ctgggcagca gacgcgacac gccgcccacc ccgaaggccg ccgacgggat gacggagagg
    16321 gccggcaggc gcagataacg ctccacgtct tcggaggagc gcacggtgtc gttcaggtat
    16381 tcgagaaaga gaccgaggcc gacgccgaag gctgtggaga gcaggaaggc gagcgccacg
    16441 ccctgcttgc gcttcggccc gacgggaaag ccgggcgcga gcgcgtagtc gatgaccgag
    16501 atgttgttgg gcgtgccggc cagcactacg tcgttttcgc gcgagcgttg gaggaggccg
    16561 tcgaggagtt ctttgttggt gccgatctct tgttggagga tgcggtagtt gatggcggcg
    16621 tcgttctggt tgagcgtctc gtctcgctgc ttgccgagcg cggcgcgcag actctgttcg
    16681 cgcgcggcgg cctgtcggaa gcgcgtctcc agattcgtca ggccgacatc tttggcgcgg
    16741 tcgcgattgg ctccgagctg acgttcgagt tcgacgatca gttggttgac ctcgagcatc
    16801 gaggggtgct cttccctgta cttctcggcg aggagcgcgc gccgctgccg cagctcgtcg
    16861 agcttggcct gcgcgtcgcc gacgtggcgg ctgccggtct cggccatcgc gtcggccgcc
    16921 ttcggctcgg aggccgcgcg gaaggcggcc tcggccagct tgcgctcgtt ctcggcttcg
    16981 aggagctggc ggttgaggcc ggcgagccgc tcgacgacga tgttctgctc gccttcgagc
    17041 gagagtatct ggtgattgcg cgcgaagttg gcgagctgtt cctcgcccgt gcggattttg
    17101 ctctggagtt cggccacgcg acgcaggagg aacgagcctg tcgaggtgtt ggtctcggtt
    17161 ttcttctcca ggttggcgag gacgaaggtg tcggcgacgg cgttgacgat cttggcggcc
    17221 acctgcgggt cggcgtggcg gtaggagatg tcgatgagcc gcgtgtcctt gacgtagccg
    17281 gcggtgcggg tctccttgac cggctcgacg cgcaggccac cctgaagcgc accgacgtag
    17341 ggcgcgagcc gccgggcctc ggccaaatct tcacgcgaga cggccggcgc cacggaggtg
    17401 gtcagtggca aatcatcgcc cgcgacttcc tgcggctgct ggccctcgct gacgatgccg
    17461 agggcgcgca gcatcccgcg cagcgaggag cggcccgcgg ccgagcgcgg gttgcgaaaa
    17521 gactggttgt gttcgaggtc gagcgtggtg gcgacgcggc gcagcaggcc cggactcgtc
    17581 agaatctgta actgcgtgtt gaagtaagtc gggtcgttga cgggattgtt gatgacgacc
    17641 gactggctct tcgccgctcc gagcgaggcc ccgaccgtct ccagatcgac ctgcacgcgc
    17701 gcctgcgcga cgaacacgtc aggctgccgc gccatgtaga cggccgccag cagcgtgacg
    17761 cagacgacga cgcccgccac cagccacaga cgctttctca ccgcgcgcca gtagtcgcgc
    17821 aggtgtacgc cctcgccctc gtcggcgtcc ggcccgtagc cggcgtaggc cggaagcgtc
    17881 agaggcgcgc gcgccgccgc gccgcctaac tccaaagccc tgtccgctgg cttagacact
    17941 tcttgcttgt cgctcatctt aaaaaccttt cagggacgac ggcgcgcgca cgccgcctcc
    18001 agagaacatc tatcttctac cgctcgggcg gccccacggc cgggggtcgc ggtcgccgat
    18061 ttcaaattcg gaggtcgaag gtgtttcgcg ggagagactt gcgacgccga ctcggctttg
    18121 gcgggatcga acgaccgaca cggcaaaccg ggcgtgtgtc gtcgcgcgaa ttggcgcatc
    18181 cttgcctttg ccggggaaca cctgtctgca catcgaaccg cttcgtctcg cgccgtctgt
    18241 cacgggcgcg cgccgcggcg gctcactcct ccagcgttct ctgtgacgag aagtttctcg
    18301 caccggggtt gagtaaaact tgagccgccg cgggaattac ctatccccat gaagcggcag
    18361 agcttaccgg ggcgggcggg acgcgctgtt cacgcctgaa cccactgcta gaccgcgact
    18421 cacctcaacc aacgactcga ttcgtcactc tcaacaaaca ccccatgacc aatgtctgac
    18481 cccgtaatcg tcgcgtcaga agtcaatcga ctatttttct cagcgccact ttgtcgtcgg
    18541 atcgccgaga agcacccacg tgcgccgcac gtccgggttg gcggttgagg agcgcgcggc
    18601 gcgcagggcg tcgccgaggc gcaacgtgcc ggtcgattca aacagcgcgc ggtagagcgc
    18661 gcggttcatc tcggcctgcg cttcgggggt ggtcatcccc gtcgaactcc acgcggcgag
    18721 cgccccgccg tgctcggcct tgagcagcga ctcggagagg ctgtcgagcg aggggtcgat
    18781 gtagtagccg ttcagacagg tcatcatcgc gaagagcggc aggcgcgcgc cgttcgagag
    18841 cgcgagcgcg tcagatgagc gcagcaaccc ctcgcccgtc cacacgccga ccgcgccgtg
    18901 gccgacgtag ttgacgagcg cggggccgga gttgacgccg ccgacgatct cggcgcgcac
    18961 ggtcgcggcg tcgcgcgcgc cacggtcgat cctctgggtc tcgtagcccg cggggacgtg
    19021 cgcgaggagc gaggaggtgg cggcctcgaa gtcgaagccg tcggctcccc tgcggtcgga
    19081 gaccagaagg agccggcgct cggccgtgcc gggcgcgtgg cccacgatct tcgagacgac
    19141 gagcgcggcc tcgttggcgg tgcgaacggg cagacgcccg acagccatct ccgcgagtcc
    19201 gtcgccgtcg aagtcggcca gccactcgtc cgaggaagtc tccatctgcg acgtgtcgat
    19261 gagacgcgcg gggacgaagt cgaagaggcc gcgcccgaga tgattccgcg ggtcgtagct
    19321 gccgtcgccg actaagagca cgtagcgcgg cgcgcgttgc cacgtcgagt gtgcgcggac
    19381 gaggaagtcg cggacggcct tgggcgtgtg cgccccgtag gagaactcgt cgtagaggtc
    19441 ctccacgtca acaaccgtgg tggccagacc ttcggagacg cggcgctcgg cgagcgtcgt
    19501 cgcggcggcg cggaagtcgg cgtgcgtgac gatgacaaag tcggccgcat tcgacgcggc
    19561 gtaccactgg gagggctggt tgacccgcgt cccatcgacg gccgagaccg cgccgccggc
    19621 gaaagccaga agcgtgcgcc ggcctgtgcc gccgggcgtg aacctaacag cgtagccgcc
    19681 gccctcgtca attttcacag actcgaccgt ggccacgact tccgtcacgg agccgcgctc
    19741 cgtgatatcg acgacgcgga tgttcgccgt ggtgaagccg gtgacgcgcg tgaccgcgcc
    19801 cccttcggcc gtgaagttca gttgatccgc ctcggcgcgg taggcgcgtc ggtaggtgac
    19861 gcggatgtag tctgtgagac tcacgtccga gggcgcggcc gacgtgagac ccaacgcgtt
    19921 gtctccctca agcagcagcg tgggcgaaac gtcgaaggcc gcggctttgt tctcgcggtc
    19981 ggcgaaggct aattggccga cctccgcgcc gttaagcatc acgcggacgg aatgtgggcc
    20041 ggccgtgacg ccctgaagcc tgacttcgag gcgggccgtg acggcgtcgg ccgggcggag
    20101 gccgcggacg gtgagcgcct gcgcgggcgc gttgggcgta atcactccgc cgaagaagtt
    20161 actcccctcg ccgttgagca gagcagaata gtagacgctg cggtcgcggc gctcgaccgt
    20221 gtagtcgaag ctggggggcg gcttcacgcc accattggcg cctgctgtgg acgccgccgg
    20281 ctgcggctca tgaccacctt tcggcgggcg tggcttcccg ccgccctggg gcgcggagga
    20341 ggtgcgcctt ccgggcgtcg tgcccacgat gagccagtag acgcgcgtgt cggcggagat
    20401 agtgtccagc ccttcgccgt agaactcgac cacgccgttc tgcgcccacg cggaggcgtt
    20461 gacgtggatg gcctgctcga cgccctcggc gaagagttgc agatacgcgg ggtcagccga
    20521 cgggctgaga ccggccgcct ccaactgtgc ccgcgtgacg cgataccagc ccggaagacg
    20581 caccgaaagc ttgacggcgg cgccggcggc cagcacgcgt tgacgctcaa ccccatcggc
    20641 gggcatgtag tccggttccg gcgcggcagg ccccgtaatg ccggacgcgg ccacgttccc
    20701 cgcgccggtg ctgtcgctct gtccttcgac gacgccgccg ccgccgctgc tgctgctctg
    20761 cccttcgacc gacgcctgcg cggcccaccc gcgctgcggg ctgccgccgc cctgtgttgc
    20821 gcctatctgt tcgagcagcg ccgaggtgcg cggtttcttc tgtcgtcgcg tctgcatgga
    20881 gacggatgtg ggccagaagg ggccgtgccg tgtgccgacg ccgtcggcgt ccacgtcttc
    20941 aagccagtag cgcgaggcgg gcgtgccggc cgcgtccgtc cacgtgtagg agccgccggc
    21001 ggtgagcgcc gtccccggcc ccacggcgag cgccgagccg gcgacgagcg agggcgtgat
    21061 gagcgtgcgc tcgcccgcct cttcgcggta gaggcggaag ccgaggttat cgatctccag
    21121 ctcggtgcgc cacgcgagcg cgacgccggc gccgtccacg agcgtcgcct cgaactcgcg
    21181 caggcgcgac agcgtcggcg ccagcacgac gtgcgctctg gaaaactcgg aggtgttgcc
    21241 cgcggggttc gtggccgtgg ccgtgatgac gttgccgagc ggggcggtgg tcgtggccgt
    21301 gtgcgtgaac gcggcgtcgc cgccggcatc cgtcgtcacg ctgatggagt cgaggtaatt
    21361 ctgcccctcg ccgtgggccg tcgggtcggg cacggcgctg tagaagaact cgatggtgaa
    21421 ggtggtgctg ggcgtcgagt tgagcgtgcc cgtgatgacg gtcgagccgg tgcgggcgtc
    21481 aatgatcagg gggaagttct ggaggttgtt ggcgccggtg tcggggtcgt ccagatcgtt
    21541 gagagtgacg ccgtctgcgc cgaggtcgat gccgagtccg atgttggagt ggatggagtt
    21601 ccggcggatg gcgttggccg tgcccgagct gacataaacg ccgtcgccgc cgttgaaggc
    21661 gatggtgttg ccggcgccgg cgaccgtgcc gccgacggtg ttcgtgttgg cggagattag
    21721 gacgccgtgt ccgacgttgg cgagtgcgct caggccgtcc gtctgagtgc cgatgtagtt
    21781 gccctgcacg ttgttcacgc tggccgtgcc ggcgatctcg acgccgtcgg agaggttgcc
    21841 cgagatgacg ttacgcagat cggaggtcgc gccgccgacg gtgcaactga tcacgccgct
    21901 caggagaaca ccgttagcga cgttcgggac agcgcccgcg ccgtcggagc ggaggccgat
    21961 gagattgccg cgcacgagat tgctgtcggc gttgcccgaa agctcgacgc cgtcgccgtt
    22021 gttgcccgag atgacgttgc cggcgctcgt gccgggcgtc gcggtcgagc cgccgatggt
    22081 gtttcccgtg gccgcgctga cgagcacgcc gttggcggtg ttgccgatgt cgccgacgcc
    22141 gtctttgtcg atgccgatgt agttgccggc gacgacgttg ccggttgcgc cgctgccgtt
    22201 gatctcgacg ccgtcgccgg cgttgttgcc gatgacgttg cgcgcgccca cgactgcgtc
    22261 ggccgtcccg tcgtcgtcgt cgtcaccgcc gatgatgttg ttgtcggcgc tttcggcgac
    22321 gcgcacgccg tcgccgccgt tgccgagccc ggtctctgtg ccgcccacgc ccgtgccgat
    22381 gtaattgccg atgaccttgt tgttgacgct ggcggtgttg acgatgtcca cgccgtcggt
    22441 cgtgccgctg gcgttatgcg ccgagatgac gttgcgcgcg gtggccgtgt agcccccgac
    22501 ggtgttcgag gcggcgctgg agataaagac gccgttgccc tcgttgcgca gcgcggtgag
    22561 gccgttcgac tggaggccga tgaggttgcc gagcacgatg ttggagttcg agttgttgat
    22621 ctcgacgccg tcggactgat tgcccgagat gatgttgccg ggcgaggagc cgggcgaact
    22681 ggtgaggccg ccgacggtgt tcgacgtggc gccgccggcg atgagcacgc cgttcgaggc
    22741 gttgccgagg tcggccgtgc cgccggagct ggcgccgatg tagttagcgc cgacgagggt
    22801 gccggtcacc cccgcgccgt tgagttcgac gccgtccgag ccgttcgagc tgatgatgtt
    22861 gcggaggccg ggggccgcgc cgccgatggt gtttccggcc gcggcgccgg tgacgttgac
    22921 gccgtcgagc gcgttagcgc gcgccgcagc gccgcccgac tggaggccga tgagattgcc
    22981 ctgaacttcg ttcgtgcccg cgccgacgat ggcgacgccg tgcgcgtcgt taccggagat
    23041 aacgttgccc gcgccctgcc ccggcgtggt cgtcgcgtcg ccgatctggt tgccggaggt
    23101 cgcgatgctg acaccgctca aggtgttgcc gaggtcggcc gtgccgccct tgtcggcgcc
    23161 gatgaagttg ccgaggacgg aattgcctga ggctccggcg ccggcgatgg tcacgccgtc
    23221 gccgtcgttg ccgctgatga cgttgcggtc ggccggggcc gagccgccca cggtgttcgt
    23281 cgagccggcg gagacacgca cgcccgagcc ggcgttgccg aggtcggccg tgccggcgga
    23341 gtcggcgccg atgaagttgc ccgcgacggt gttctggccg gcggcgccgg agagttcgac
    23401 gccgtcgccc ccgaagcggt tgatgatgag gccgcgcagg gtcgtgctgg tagtggcaat
    23461 ctggaggccg tcggctccgg ctccggcgct cgtgccgttg agttcgatga ggaggacggc
    23521 gttgtcggaa gcggcgaggc tgttggggag cgtgccgggc tgcgtgtaac cgtcgatggt
    23581 gactggctgt gtgacggccg gtagcggcga ggccggggag atggttcgca cgccggcggc
    23641 cgggacgagg atgtcgaagg cgatggtgtc ggcaatcgcg tcggcgttgg cctgctggat
    23701 ggccgcgcgt agcgtgcact cgatgacggg cgggacgccg acgagcgcgc ctgtggagca
    23761 caagccgtca ccgagattgg agtctggggc gtcgccggtg gaattgacga tgaaggtcga
    23821 ggcgaagggc gccggccgcg ccgacgcgtg tcgataggtg acggagcaga ccgcgaacag
    23881 gagcgccgcc acggcaaccc cgagggtcgc gcccggcacg cgcccgtcct tcgcgggtgc
    23941 gcgcgtggcg aggccggcgg cggcgagtgg cgggcgggcg gaacttttca tgtctttcac
    24001 ttcgagcctt tgcagcggac tcttcggggc gcttcttcgg cgcgccccag cgcggattac
    24061 ctacgaacga cgctcgcccc gcgccccggc cgacaggccg gagacgaacc tcgcggtgcg
    24121 cacgagcacg ttgccgacct gaaaaggcaa acgcggcaat tcgttgaatg ggccgccgag
    24181 gcggatggtc gcctcgatgg ggtttggcca gcggttcgcc aaatccttca gcaccccgcg
    24241 ggggcggcgc agataatggg tcatcggcgc gcgatggcgc atcggcgtct ggaggtgtgt
    24301 gtagggcgtc tcccagctct tcataacttc cgggacgagc cagcgcggga ggcgacgcga
    24361 ctcgcgctcg aacggcgtgt cttggatgcg cgcgccgagc aattcgtgtg cgaggccgac
    24421 ggcacaggtg acccagcgcg cccgtcgccc gtcgccccgc agcagcctcg accagtcgaa
    24481 gccctccggc cgatgctcga cggcggcggc cacgtcgcac agccacagcg gcctccacgc
    24541 cccgtgccgg agcaggtgaa cacacaggag ccgcaggtga tcttcggggc agagcacggg
    24601 cacgtccaca ccgccgaccc tcaacacctc ggcgcgggcg aagagttcgt catcgccgcg
    24661 cccggccgcg tcctcgaatc ccctgtgcaa atccactgag aactcctgcc cgcgggggcc
    24721 gctcaacacc gccgcggcta cggcgaactg ttcggggcgc acgcaaacgt cgatgtcgcc
    24781 aaagggccgc agccccgcct ccgggtagag gcgcgaggcg gccagcccct tgacgaggac
    24841 cgcatcgacg cccgactcgc cgagcagttt gaagacctgt tcgatgcggc gctcgtggag
    24901 ggccgactgg agtgtgtgga gccggtaggc ttgtcgcagc tcttgcgcgg cgggcgcgtc
    24961 gcgcaactcc gtgtggcgaa ttttccacca gccgagtgcg ccagcgcccg agccgagaag
    25021 caggggcgcg accgcgtcga ggtcgttcgc cgagcctccg aatgccggtg gcgacggtcg
    25081 ccacgcgccg gccagagcgc tggcgacgag gcgcgcggta gattgagggg ggacggccac
    25141 agtcttgatc aaaagctaat gcgaagagac cttgccgggg aaagtttgat ttagtgagcc
    25201 gcgcggccgc cgcggggcgg cgctcaggcg caggcgccgt tcggggcgcc ggggcaaagc
    25261 tggctgcaac attcaacggg ggaggtgcag gccataccgc tttgcaaaca actggccgcg
    25321 gcctttgccg tcggggcgag tatcgaggtg atgagcggca gggcggcggc cacgccgatc
    25381 cggcgaacga ggtcgcgccg cgaaatggcg gcccgccccg gcggccgtct cacccgctca
    25441 cgcaggaggt gttcacgtcc cagacggtcg agcgcaagcc acaccgccga ctccgcaaga
    25501 ggcgcatcta actcatcgtg gaggagccgc gccagcccgg cgacggtcgt gcgcccgtcg
    25561 caccctctcc agacgagcgc cgccgtctgg ttcaggcaaa tggcccgatg gcgttcgggg
    25621 tcgtagacca aaacttcgcc ctccgcctct ctgacgatca gcccctccgt gcgcgcttgt
    25681 ggcgctgtgg attcctcgtc cttcaacttc attacttaat gcgatatttt gccggctgat
    25741 tctaagcgcg gtatggcccc gccgcaggac gggccacact ttcggcaacg gagccgacgc
    25801 cggcaggcgg ctaagcgcaa atgttgctta acaggcagtt attggacgca cactggttgt
    25861 tgctgccgca tgggcagcct gccggcctgt tagtaccgcc ggaacagttg agcgacgcct
    25921 gcgcctgcgc gctcggggcg gtgatggcca tcacgaccgg cagtgtcagc gccgccacgc
    25981 cgacccggcg caggaggtcg cggcggcgga tcgtgcgcgt cgtggcggcg cgcgtgtcct
    26041 cacgtccttc gagcagatta atcttgtcca gttggccgag ggcgtaccac accacatcta
    26101 cgtctacggc cgcgccgagg tcgtcaccga ggaggcgcgt cagttctccg acggtcgtcc
    26161 gcccgtcgca acgcttccag acgagtgcgg cggtgctgtt gaggcagagg gccttgtccc
    26221 ctttgagatc gtagaccagg acctcgtcgg gcagttcgcg cacgagcaga ccgtccttac
    26281 gcgcgagtgg cttcagctca cgatgctttt tttccataag tcccctgagg cttttctccg
    26341 caaactttaa accgcggctt tttaggcgcc gtcgtgtgag gggctactga cgttgaaatg
    26401 ttcaatgacg ccgaaccttg gatgcttgcg cgaattaatc agggcgcgtc gtcggccccg
    26461 gctggaaccg tttcgcgccc agcgcgaccg gccccgactc cacagcctct aaaattgcct
    26521 cggccgccgc gcgcgcctcg ccgcgtgtgc tcttgatgac gggcgcgccc gccgtgacct
    26581 gttgaagggc gtcgagcgca gcttcgggcc gccgcctgac cgatatcgtg ttggagagca
    26641 gtgtcagcag gccctgcccg ctcgacaaac ggcgcggtcg ccagcgcgag cccggacgat
    26701 acttgctcac cacgacaagc cccactggca acggccggct gccgctcgtg ccgtcaagcg
    26761 attcgactgt caccctgcgc tgccggccgc tttgcgtatc cctgatcccc agcgggcggg
    26821 cgaaaggatg aacgcgcccg cgccggtcga gcacggcgta ctcgtcggag taataggtgg
    26881 ctccggcccg cacgagttcc gcgaccagag tgctcttgcc cgaaaggctg ttgccgggca
    26941 gcacgattgc ccgcccacgc cacccaacca cgccggcatg cacgaagact ctccgccgcg
    27001 cggcctccgc cacgtaaagg tggaggtctg cggcgaatcg ttcaaacaac tcttcgagtt
    27061 cgtgcgtccg cgcgatcctc aagatgcctt cgtacaaaag gctgaaccgc ctgacgcgcc
    27121 cgcccggcgg cgcgtcgggg gcggcgacga gcgagtacag acgctcaacg acaccggccg
    27181 tcaccggctt ccaccccggc ggcaggtatt cggccagcgc cgggagcacg ccgggccggc
    27241 tggcgcgcag gccgacgcgc acgccgtaac tctcgaaggt gaatgactcc gcccagccca
    27301 ggcggtcgag tttcctcatt caagtcgagg aggtcttgag tcgaggcaca atcgactcgg
    27361 gcatgacacg agttccgtcc atctgaaaac ttccgcgccg ggagtgtggg ccgcgagcga
    27421 attatatgtc tgacgcggct tagggttttg aaatgaccat cctgacgcgc aagtaaactg
    27481 cccgttggat ggagcggcgg cacgtcggct gaagggagcc gactggcgca cctgccgggg
    27541 tagcttaaag agtgagaaaa ctaacactga aagcgcgccc cgtcaaattt tttaacgcgg
    27601 cgacaggatg gtcacgagag gcttcgggcc ggcgacttat cagcgcggac gcgggggcgc
    27661 cgggggcctc accagaatct cgtcgagaac ggccgcgagg cgatagccgg cgagcgcaat
    27721 ccgccgcccg acgatgaccc tcgactcttc gatatactgc gccgacggag cctcgccttc
    27781 cttcgtcttc acataagcaa aggtgcgcgc gatctggttg ctctcgatga cccacgcctg
    27841 cgggtcggtc tgcttccagt cgtcgcccgt gggcggatat ttcttcatca ggtcatcggc
    27901 gagctggcgg atgcgcgcgc gcccggccgc gtcgagcgga cgcttgaggt cgtcgtagcc
    27961 gaagaggccc gcgccgttgt cccagaagga gtgaaggttt ttgtgttttg agccggcggg
    28021 cggctgcatc gtgaagaagt tgcctcccgc gtcgccgtcg ggattcttgg ccgagtagcg
    28081 cgtggccgcg tgcagcggct ggtgcacgtc gccgacgaga tgatagagga agccgagcac
    28141 ttcggcgtcg cgcttgtcgc tgcctgtgcc cgtgcgcagc gtgttgatgc accagttgag
    28201 gcggtcgagg acattgatgg gaagcgggcc gacgttcgcg cgctcaggcg tgccgtcgaa
    28261 gatggggcgg tagttgatgt agtgccagtt gtcgtaggag tcggtcagcg agtcgccgcg
    28321 gaagtcgtcc atccagacgg cgatggtcac ggggtcgtaa atcttctcgc aggtgtcgcg
    28381 cgtgtagtag ccggcgcaga ggtggatgag cgggcggcgg tcggcgggcg cgacgaggag
    28441 gcgatccacc tccgctttcg ccgccggcga cagacgcagg taagcgatct gcgccgcgag
    28501 catgtggccc gtctcgtgcc acgcgagcac ctgcgcctgc ggcacgaaca gcgccgcgca
    28561 aagaattaac acacgccagg ctcttttcat catgcctcct gtaaataaca gtaccgcgag
    28621 cggaagcctc gcgggtctgc ttgcagtggg agtttgtctt gcgttccgta gacccgctcg
    28681 ctaccgctcg cggtactgtc ccggcagtgc cagttcacct gtagattaaa aatttcgcgc
    28741 gcgcgttttt gaattcgtcg aggcccgcct gccacgagcg catgacctct tcggccgacg
    28801 cgcccgagcg gacgcgcgcg agcgtgtcgg cgttagcgag caggcggagc atgtcgtcca
    28861 ccttccactc ggccgggtgg aaacgccgga gcgcgacggc caactcgatg ccgacgcgca
    28921 cgggccggaa cgcggcgcgg tcggtgacaa taaagttgac gccgccgcac tcctcgccct
    28981 tgtaaacgga cgccttcggc gtgaagcgca cgggcacgaa acgtatgccg gggagacggc
    29041 gcgcgttcat gtgcgcggcg aggcgctggc cgtcgatcca cggcgcgccg agcacctcga
    29101 agggcgtgtc ggtgccgcgt ccgaccgaga cgttggtcgt ctcaaggagg cccacgccgg
    29161 ggtagagcgt cgcctgcgtc aggcttcgca tgttcggcga ggggttgacc cacgtgaggc
    29221 ccgtcgagtc gagccacatc gcgcgccgcc agttctccat cttcacgacg cgcaggtcgg
    29281 cgccgaactt ccgttgctcg ttgaagagct gggcgagttc gccgactgtc atgccgtggc
    29341 ggacggggag ggtgtggtag acgacgaagg agagcttgtc ggcgtcggcg attggccctt
    29401 cgacctccgt gccgccaatc gggttcgggc ggtcgagcac gtagacgggc ttgccggctt
    29461 tggccgcctc ctccaacacg tggccgagcg tggagatgta cgtgtagaag cgcgcgccga
    29521 tgtcctgaat gtcgaagacg acggcgtcga ggtctttcag gtgctcgggc ctggggcggc
    29581 gcgactcgcc gtagagcgag tagatgggga gcctggtctt ttcgtctaca tcgtccgaca
    29641 ccttctcatc ggcgaggccg cggatgccgt gctcgggcgc gaagagggcg acgaggcgca
    29701 cgccggcggc ggcgtggagc acgtcgatgg tcgggcggcc ccgccggtcg cgccccgtgt
    29761 ggttcgtcac cagcccgacg cgcatcccct gcaactgttt gaagccgtcg cgcgcgagca
    29821 cgtcgatgcc ggtcaggact tccgcatcgg ctggcgactc ggccgtcgtc gccgctgacg
    29881 cctcggcgcg cgcgccttca gccgccgacg tgaagcgaac aaggttcatc agcatctctt
    29941 gcgtgtagcg agactgttgg agccgcgagt gcgcgaccgt gccagcgtcc gtgaccgccg
    30001 aggcgacgat tgacgcgacg cggctgcgca agcttcccac gtcgcccttg ccgtcggggt
    30061 gaacgcggtt gctcaggaag acgacgaagg tctcgctcgc ggggtcgatc cacagagagg
    30121 tgccggtaaa gcccgtgtgt ccgaaggagc ctgtgggaaa gaggtcgccg cggttggccg
    30181 agaagctgct cgtgatgtcc caaccgattc cgcgcgcctg cccctcctcc gtcacctgac
    30241 gcggccgggt catcgcttcg acgccgagcg gactcaggat acgcacgccg ttatattcgc
    30301 cgccgttcaa aatcatctgg caaaaaatcg cgaggtcgtc ggccgtggaa aagagaccgg
    30361 cgtgcccggc gacgccgccc atgcgattcg aggtcgggtc gtgtacctcg ccgcgcagca
    30421 agcgctcggc gtcggcgccc gccgcgcccg tcccgccgag atagctccgc atcccgcgca
    30481 cggtctcggt cggcgcgacg cgcgtcaaaa gctcacgctt ggggcggaag cccgtgtcgc
    30541 gcatccccag cggctcgtag atgttctttt tggcgaactc gtcgagcggc agacccgaga
    30601 cgcgttggac gacctcggcc gtcgcgatga ggccgatgtc gctgtagacg aaacgcgcgc
    30661 ccggcgggct gcgaagcttc tccacgtaga gccgcttgag catctcttcg tggcccgtcc
    30721 actgctcctt gaggtcgaag tcgggcgcgt agcccgagcg gtgcgtgagc aggtgctcga
    30781 ccgtcatgcg gtcgcggccc tcgcccttga gttcggggat gtagcgcgag accggatcgt
    30841 tcagacgaat tttgccgcgc tcgactaaga tcatcacact cgtcgccgtc gccaccactt
    30901 tggtcaggct cgcgaggtcg aagatggtgt cggcggtcat cggctcgcgc gcgggggcga
    30961 cggcgcgcgc cccgtatgcc ttgcgccaca tgacgcggcc cttgcgcgcc gcgagcacca
    31021 ccgcgcccgg cgtttcctta cgctcaatcg cctgcgccac caccgcgtcg atctccgcga
    31081 gacgcgcggc cgacatcgcc gtggcggc
//
LOCUS       DASVES010000110         6464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_37390, whole genome shotgun sequence.
ACCESSION   DASVES010000110 DASVES010000000
VERSION     DASVES010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37390"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3178
                     /locus_tag="VF240_04070"
     CDS             <1..3178
                     /locus_tag="VF240_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7174444.1"
                     /translation="FEKTSTPMVNAAAKKVSSLLDGVRPRWGGGRLTEIIDRAKETVD
                     RVEELTHETKEAFELFRPFMVENAYIFRADNVRSLFASVRKDEQQLLPWRVEQLDWYD
                     YWMRVHFPGLQKWVFPKLEEDLRAVEKRSYTYRDLMELFETSAKRFSTRVAMRIERDG
                     RHEQYTYADLRELATRAAAFLVSRGVKPADRVLLVSHNMPEWGMTYFGVLKAGATCVP
                     VEPESSTEEVLNFARASGATGIVFGPKALETHPDLRARLDAAGLAETQVWTFDEVFEL
                     PDEKTEDQRIALLPQRIPPNSLASLIFTSGTTGSPKGVMLTHKNLSNMVSMLSSVFDM
                     NTSDGVLSVLPLYHTFEFSTGFLTPLSRGAQITYLDELNGEELARAIRNGHVTGLVGV
                     PALWEMLHRRIHNRLHERAWVGRMADLLMRANSWLRDRTPLNLGRVFFHPIHAGMGGR
                     IRYLISGGSALGEQVKRDMHGLGFTILEGYGLTEASPVLTVTRPANSLLADSVGRPLP
                     GVEIRITDPDESGIGEVVARGPNVMAGYYQNEEATRHALSGRWLRTGDLGRLDDDGNL
                     YLVGRSKEIIVDTNGKNVYPDEIEELYGASPYVKELSVVGLTESLGEKVACLVVPDYD
                     HDIALSREDVRRRIEEHFRDVSSSLPFYKRVKVLHFSDDELARTSTRKVKRREVVRVM
                     EELELRLRPGLGEGPARHDRSADASWLIDIVASVANRPRAQVSISTRLSELGFDSLMF
                     VELAAAIETAGGAISSPETLNNVQDVGELLSAVTRRPTAAERHAAERRAAESARREDE
                     EIHVPSAVRVLGNRGLDVIQSLFYFRVLDSKIEGQSNIPVHTNFIAASNHASHLDIGL
                     VKVALGEYGRDMVALAAADYFFDNKYKRAYMNNFTNLVPIERSGSLRQSLRHARSFLE
                     RGYTALIFPEGTRSMTGEMAEFKSGFAHLALSTRTGILPIYIHGTYEAFPKGSNMLKG
                     REVGARIGRFLPPEELEDLTRGMPKSEAYRLVAALVRHEIENLRDGTRHAFDAKRLRR
                     RWRHERRAGSAEDPEGTQPAGELATTGD"
     gene            3462..4583
                     /locus_tag="VF240_04075"
     CDS             3462..4583
                     /locus_tag="VF240_04075"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7174445.1"
                     /translation="MVTKKATKAAPRAASKKAAKKGAKTAGSKALVRQRAPVTLVTGG
                     TGFLGAHLVRELVAAGVKGLRVLSTSRPAWLEELGVESVAGSITSPEDVARAVEGAAE
                     IYHLAGRVSREPEDAHLMYDLHVTGTRLLCEAAARAGVGSIVLASTSGTIAVTEDGEA
                     LPDEEWPQPADIVSRWPYYASKIYQERVALEHFSGDGRRLVIVNPSLLLGPGDERLSS
                     TKVVLDFLSRKIASVPKGGLSFVDARDVAHTLTKAMECGAHGERYLLGAANWTFEEFF
                     GRLERQTKVAGPRLALPSKLTLAGARGLHALYRHWKLAPPVEPSEIEMADHFWYLDSS
                     KAARELGFAPRDPAVTLHDTINYVRENFLGNSAFTSAGR"
     gene            complement(4584..5576)
                     /locus_tag="VF240_04080"
     CDS             complement(4584..5576)
                     /locus_tag="VF240_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7174446.1"
                     /translation="MKAQKRDSAKEKVEMSSLPLVIVNPASAGGATGEAWPGVASDLR
                     RHFGPFACAFTERSGDARVIAEHEAARGRSFIIACGGDGTVNEIADGLLRSGADAELG
                     VLPSGTGGDFRRTLEIPERAADAARALRDGRTMRVDAGRVVFAGESGGEETRHFINVA
                     SFGMGVEVIGRVKKDGGSGATAAATRALGGKAAFAVAALQSAFAYAQPLVRITLDGGP
                     EFRLKITNFCVANARFFGGGMKIAPEAKLDDGLFDVVAIGDMSGTRILTNSYRVYLGT
                     HLGLRQVQHTRARRVLARADDDGEVGIEIDGETAGRLPATFEILPRSLRVRAPR"
     gene            complement(5536..>6464)
                     /locus_tag="VF240_04085"
     CDS             complement(5536..>6464)
                     /locus_tag="VF240_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7174447.1"
                     /translation="FMGWEQVRPTRDEIGVLSPFERFAFRLTRRMNTGRWKEFWTFCQ
                     RHLGSLWIHLATYNLMRVYGIEHVEHVSHERPLLLVANHRSFFDMYVVSAVLFRRTRA
                     PVRLFFPVRGRFFYDSPAGLLVNLLMGWWSMFPPFFASAERPQIEKRAFDKYSLRLLA
                     ALCREGRGHVVGFHPEGTRNKSDDPYSFLRAQPGVGKLIREASPQVIPVFIAGLGNDL
                     PRQVLGNWRGGEPVRVHFGPPIDFAAFNDRGESVRVYKETADYVLTKIAELGEQDRSM
                     YQLGVGQQEATLPRPASRVDEGAEARQREGKS"
BASE COUNT         1122 a         2235 c         2077 g         1030 t
ORIGIN      
        1 cttcgagaag acctcgacgc cgatggtcaa cgccgcggcg aagaaagtct ccagcctgct
       61 cgacggcgtg cggccacgtt ggggcggcgg ccgactcacc gagatcatcg accgcgcgaa
      121 ggagaccgtt gaccgcgtcg aagagctgac gcacgagacc aaagaggcgt tcgagctgtt
      181 ccgccccttc atggtcgaga acgcctacat cttccgcgcc gacaacgtcc gctcgctctt
      241 cgcctccgtc cgcaaggacg agcagcagct tttgccgtgg cgcgtcgaac agctcgactg
      301 gtacgactac tggatgcgcg tccacttccc cggcctccag aagtgggtct tccccaagct
      361 cgaagaagac ctccgcgccg tcgagaaacg ctcctacacc taccgcgacc tgatggagct
      421 gttcgagacc tcggccaagc gtttctccac gcgcgtcgcc atgcgtatcg agcgcgacgg
      481 ccgccacgag cagtacacct acgccgacct gagggagctt gccacacgcg ccgccgcctt
      541 cctcgtctcg cgcggcgtca agcccgccga ccgcgtgctg ctcgtgagcc acaatatgcc
      601 cgagtggggc atgacttact tcggcgtgct caaggccggc gcaacttgcg tgcccgtcga
      661 accagaaagc tcgacggagg aggtgctgaa cttcgcgcgg gcgagcggcg ccacaggcat
      721 cgtcttcggc ccgaaggcgt tggagactca cccagacctg cgcgcgcgtc tcgacgcggc
      781 gggcctcgcc gaaactcagg tctggacgtt cgacgaagtg ttcgagctgc ccgacgagaa
      841 gaccgaggac cagcggatcg cgctcctgcc gcagcgcatc ccgcccaaca gtctcgcctc
      901 gctcatcttc acctcgggca ccacgggcag ccccaagggc gtgatgctca cgcacaagaa
      961 tctgtcgaac atggtctcga tgctctcctc cgttttcgac atgaacacga gcgacggcgt
     1021 cctctccgtg ctcccgctct atcacacctt cgagttttcg accggcttcc tgacgccctt
     1081 aagccgcggc gcgcagatta catacctcga tgaactcaac ggcgaggagc tggcgcgcgc
     1141 catcaggaac ggccacgtca cggggctggt cggcgtgccc gcgctgtggg agatgctcca
     1201 ccgccgcatc cacaatcgcc tgcacgagcg cgcgtgggtg ggccgcatgg ctgacctgct
     1261 gatgcgcgct aacagttggc tgcgcgaccg cacgcccctc aacctcgggc gcgtgttttt
     1321 tcaccccatc cacgccggga tgggcgggcg catccgctat ctcatcagtg gcggctcggc
     1381 gctcggcgaa caggtcaagc gcgacatgca cggcctcggc ttcaccatcc tcgaaggcta
     1441 cggcctgacg gaagcctcgc ccgtgctcac cgtcacgcgg cccgccaaca gtctgctggc
     1501 cgactcggtc ggccgaccct tgcccggcgt cgaaatccgc atcaccgacc cggacgaaag
     1561 cggcatcggc gaggtcgtcg ctcgcgggcc gaacgtaatg gccggctact atcagaacga
     1621 agaggccacg cgccacgcac tctctggccg ctggctccgc accggcgacc tcggccggct
     1681 cgacgacgac ggcaacctct atctcgtcgg ccgctccaaa gagatcatcg ttgacacgaa
     1741 cggcaagaac gtctaccccg acgagatcga ggagctttac ggcgccagcc cctacgtcaa
     1801 ggagctgagc gtcgtcggcc tcaccgagtc gttaggcgag aaggtggcct gtctcgtcgt
     1861 ccccgactac gaccacgaca tcgcactctc tcgcgaagac gtgcgtcgcc gcatcgagga
     1921 gcacttccgc gacgtgtcct catcgctccc cttctacaag cgcgtcaagg tgctccactt
     1981 ctccgacgac gagttggcgc gcacctcgac gcgcaaggtc aagcggcgcg aggtcgtgcg
     2041 cgtgatggag gaacttgagc tgcgtctgcg tcccggcctc ggcgaaggcc ccgcgcgcca
     2101 cgaccgctcg gccgacgcct cctggctcat cgacatcgtc gcttcggtcg ccaaccgtcc
     2161 gcgcgcacag gtctccatct cgacgcgtct ctccgaactc ggcttcgaca gtttgatgtt
     2221 cgtcgagctg gccgcggcca tcgagacggc cggcggcgcc atctcctcgc ccgagacctt
     2281 gaacaacgtg caggacgtgg gcgagctgct ctcggccgtc acgcgccggc ccacggcggc
     2341 cgaacggcac gcggccgaac ggcgcgcggc cgagtcggcg cggcgcgagg acgaggagat
     2401 tcacgtcccc tcggccgtgc gcgtgctcgg caaccgcggc ctcgacgtga tccagagcct
     2461 cttctatttc cgcgtcctcg actcgaagat cgaagggcag tcgaacatcc ccgtccacac
     2521 caacttcatc gcggcgtcga accacgcctc acacctcgac atcggactgg tcaaggtggc
     2581 cctcggcgag tacggccgcg acatggtggc gctcgccgcg gccgactact tcttcgacaa
     2641 caagtacaag cgcgcctaca tgaacaactt cacgaacctc gtccccatcg agcgttcggg
     2701 ttctttgcgc cagtcgctcc gtcacgcgcg ctccttcctt gagcgcggct acacggcgct
     2761 catcttcccc gagggcacgc gctcgatgac gggcgagatg gccgagttca agtccggctt
     2821 cgcccatctc gcgctctcga cgcgcacggg catccttccc atctacatcc acggcacgta
     2881 tgaagcgttc cccaaaggct ccaacatgct caagggccgc gaggtgggcg cgcgcatcgg
     2941 ccgcttcctc ccgccggaag aactcgaaga cctgacacgc gggatgccca agtccgaagc
     3001 ctaccgactc gtcgccgccc tcgtccgcca cgagatcgag aacctccgcg acggcacccg
     3061 ccacgccttc gacgccaaac gcttgcgccg ccgctggcgt cacgagcgcc gcgccggttc
     3121 agcggaagac cccgagggca cacagccggc gggcgaacta gcgaccacgg gggactgaaa
     3181 tagttgtgat gagtgataag taacgggcgc cacgagcgcc agaggcgcga aagaatgtag
     3241 cccacggcgt gagccgtggg gaagcacgcc ggaaacgatt tgaagccccg gaggggcgaa
     3301 agaatctcga cgtgcgtgac gctcaggctt gcgattcttt cgcccctccg gggctaacat
     3361 ttaatcactg acgctgaccc acggctcacg ccgtgggcta cgttctttcg ccgctccgcg
     3421 gctgattaaa cactcatcac tcatcactca tcactttttt tatggtaacg aagaaggcga
     3481 caaaagccgc gccgcgcgcc gcgtcgaaga aggcggcgaa gaagggcgcg aagacggccg
     3541 ggagcaaggc gctcgtgcgg cagcgcgcgc cggtgacgct cgtgacgggc gggacgggat
     3601 ttcttggggc gcatctggtg cgggagctgg tggcggcggg ggtgaagggg ttgcgcgtgc
     3661 tgtcaacgtc gcggccggcg tggctggagg agttgggcgt cgagtcggtc gcggggtcga
     3721 tcacgtcgcc ggaggacgtg gcgcgagcgg tcgagggcgc ggcggagatt tatcatctcg
     3781 cggggcgcgt ctcgcgcgag cctgaagacg cgcacctgat gtacgacctg cacgtgacgg
     3841 ggacgcgcct gctgtgcgag gcggcagcgc gcgcgggtgt gggttcaatc gtgctcgcct
     3901 cgacgagcgg gacgattgcg gtcacggagg acggcgaggc gttgccggac gaagagtggc
     3961 cgcagcccgc cgacatcgtc tcgcgctggc cctactacgc gagcaaaatc tatcaggagc
     4021 gcgtcgcgct cgaacacttc tcgggcgatg gtcgccgtct cgtcatcgtc aacccgagcc
     4081 tcctgctcgg ccccggcgac gagcggctga gttcgaccaa agtcgtctta gacttcctct
     4141 cgcgcaagat cgcctcggtg cccaaaggcg gcctgagctt cgtggacgcg cgcgatgtgg
     4201 cgcacactct cacgaaggcg atggagtgcg gcgcgcacgg cgagcgttac ctgctcggcg
     4261 cggctaactg gacgttcgag gagttcttcg gccggctgga gcgacagaca aaggttgcgg
     4321 ggccgcggct cgccctaccg tcgaagctca cgctcgccgg cgcgcgcggt ctccacgcgc
     4381 tctatcgtca ttggaagctc gccccgcccg tcgagccgtc cgagatcgag atggccgacc
     4441 acttctggta tctcgactcg tcgaaggccg cgcgcgaact cggcttcgcg ccgcgcgacc
     4501 ccgccgtcac gctccacgac acgatcaact acgtccgcga aaacttcctc ggcaactctg
     4561 ctttcacgag tgccggacgt tgattaacgc ggcgcgcgga cgcgcaggga gcgcggcagg
     4621 atttcaaacg tcgcggggag ccgcccggcc gtctcgccgt cgatctcaat accgacttcc
     4681 ccgtcatcat cagcacgcgc caacacacgg cgcgcgcgcg tgtgctgcac ctgtcgcagg
     4741 ccgaggtgcg tgccgaggta gacgcggtaa gagttcgtga gaatccgcgt tcccgacatg
     4801 tcgccgatgg cgaccacgtc gaacagcccg tcgtcgagtt tcgcctcggg ggcgatcttc
     4861 atgccgccgc cgaagaagcg cgcgttggcg acgcagaagt tcgtaatctt cagacggaac
     4921 tccggcccgc cgtcgagcgt gatgcgcacc agcggctgcg cgtaggcgaa ggcgctttgc
     4981 agggccgcga cggcaaacgc cgccttcccg ccgagcgcgc gcgtcgccgc ggccgtcgcc
     5041 cccgacccgc catccttctt gacgcgcccg atgacctcca cgcccatccc gaacgacgcc
     5101 acgttgatga agtgccgcgt ctcttcgccg cccgattcgc ccgcgaagac gacccggccg
     5161 gcgtccacgc gcatcgtgcg cccgtcccgc aaagcacgcg ccgcgtcggc cgcacgctcg
     5221 ggtatttcga gcgtgcggcg gaagtcgccg cccgtcccgc tcggcaggac tcccaactca
     5281 gcgtcagcgc cggagcgaag gaggccgtcg gcgatttcgt tgacggtgcc gtcgccgccg
     5341 caggcgatga tgaaactccg gccccgcgcg gcctcgtgct cggcgatgac tcgcgcgtcg
     5401 ccgctgcgct cggtgaaggc gcaggcgaag gggccgaaat gccggcgcag gtcggaggcc
     5461 acgccgggcc acgcttcgcc cgtggcgccg cccgccgacg ccgggttcac gatgaccagt
     5521 ggcaaagacg acatctcaac tttttccttc gcgctgtcgc gcttctgcgc cttcatcaac
     5581 tcttgacgcg ggccggggca aagtcgcttc ctgttgcccg acgcccagtt gatacatgga
     5641 gcgatcctgt tcgccgagtt cggcaatttt cgtcaacacg taatcggccg tctccttgta
     5701 gacgcggacg ctctcgcctc tatcgttgaa tgctgcaaag tcgatgggcg gcccgaagtg
     5761 aacacgcacc ggctcgccgc cgcgccagtt gccgagcacc tgtcggggga ggtcgttccc
     5821 cagccctgcg atgaagacgg ggataacttg cgggctggcc tccctgatga gtttgccgac
     5881 gccgggctgc gcgcggagga atgaataggg gtcgtccgac ttgttgcgcg tgccctcggg
     5941 atggaagccc acgacgtggc cgcgtccctc gcggcagagc gcggcgagca ggcgcagcga
     6001 gtacttgtca aaggcgcgct tctcgatctg cggcctctcg gccgacgcga agaagggcgg
     6061 gaacatcgac caccagccca tcagcagatt gacgaggaga ccggccggcg agtcgtagaa
     6121 gaagcgcccg cgcacgggga agaagagacg cacgggcgcg cgcgtgcggc ggaagaggac
     6181 ggcggagacg acgtacatgt cgaagaacga gcggtggttg gcgacgagca ggagcgggcg
     6241 ctcgtgtgac acgtgctcga cgtgttcgat gccgtagacg cgcatcagat tgtaggtcgc
     6301 cagatgaatc cagagcgagc cgaggtggcg ctggcagaac gtccagaact ccttccagcg
     6361 ccccgtgttc atccggcgcg tgaggcggaa ggcgaaccgc tcgaagggcg agaggacacc
     6421 gatttcatcc cgcgtcggcc tgacctgttc ccagcccata aaag
//
LOCUS       DASVES010000111        12116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1211203, whole genome shotgun sequence.
ACCESSION   DASVES010000111 DASVES010000000
VERSION     DASVES010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211203"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VF240_04090"
     CDS             <1..373
                     /locus_tag="VF240_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174448.1"
                     /translation="NEWMHLLLEAARDVSRTIPEGETFILVDEQGFGQDFARGRRVLP
                     FLERDGVYWGAPPDGATAVRELERMRRERGAGFIAFGWPSFWWLEHYAALAAHLRARY
                     ECVLENRRLVVFDLHDRGINK"
     gene            370..1266
                     /locus_tag="VF240_04095"
     CDS             370..1266
                     /locus_tag="VF240_04095"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX7174449.1"
                     /translation="MTRPKPLFIMGNKRSGSTLMTDLLNSHANVFVSHESDIAWILYQ
                     SRNGRPTRYQTHPLDSRLMLDSTLESCRRILKETLSGEPTHAEIDEAFYRAQDRLMNQ
                     YLRPSLKQRLKRAAKITGKRPTPGRVLRALRHDPELLQKIDLAWTGDKKHAQHLDPAV
                     RQFLQTHFPEARYIHVIRHPRSVVASTMEAARRWSERPEYFNGLAEQILEQWAVHEEW
                     VLEAKEREASPILTVRLEDLWAEPVETMSGILCFLDLEMTDAFAGLVKQIVYPKDPNI
                     KYASFPLPDVPRARRIMELYSY"
     gene            1329..3080
                     /locus_tag="VF240_04100"
     CDS             1329..3080
                     /locus_tag="VF240_04100"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HEX7174450.1"
                     /translation="MIGPYGGRLVSLLVPEGEREAVRERAAAALPRVQLTPRSVCDLE
                     LLATGAFSPLGGFMGRADYERVLEEMRLASGALFPLPVTLPVAADANVKVDEEVALVD
                     QHNNLLAVMRVEEVFEREDEREAALACGTRDARHPLVAEMNSWGRLYVAGRPRVLELP
                     RYYDFRNLRLTPAEVRRKLSSLGHENVVAFQTRNPLHRAHEELTRRAAEAARGSLLLH
                     PVVGLTKPGDIDHFTRVRSYKVLAERYYDPSRMLLALLPLAMRMAGPREALWHAIIRR
                     NFGANHLIVGRDHASPGLDSRGRPFYGPYDAQELVARHEAETGVRAVPFNELVYLPEE
                     DRYEEASRIPAGRRTASISGTEVREEYLRRGRQLPRWFTRPEVASILTEAHPPRHRQG
                     FCLWFTGLSGAGKSTTANILTVKLLEHGRQVTVLDGDVVRTHLSKGLGFSKEDRDTNI
                     RRIGFVASEIVRHGGGVICAAVSPYRATRNECRAMVAADRFIEVFVDTPLEVCEERDV
                     KGMYALAREGKIKNFTGVDDPYEPPLNPEIVIDTVRRSAEENAELILAYLVSQGLVLS
                     SAAQAEEDESEKLAATK"
     gene            3214..4017
                     /locus_tag="VF240_04105"
     CDS             3214..4017
                     /locus_tag="VF240_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174451.1"
                     /translation="MLFTDRFVYVHGPKTGGTFVTSMLFRLYGLRWTRWAHARNALFG
                     EVRRRHPGYGALVHNSNKHGGCNEIPPPQRGKRILATVRNPFDLYVSQYEFGWWRRAE
                     FLRYYEALPRFRDEYPSFPDLSFAEYVQLSNAALSPLSGGVRADGDARPGLITEQFLN
                     FCFRDPRAAYERLRARRYAAPKDAAADMHDIHFVRTDQLNRGLYDFLVSEGYDPGDVS
                     FVLDEGKILPRGRGRGATQKWQHYYTPELKARVREREDFLFRLFPEFDV"
     gene            4040..5254
                     /locus_tag="VF240_04110"
     CDS             4040..5254
                     /locus_tag="VF240_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174452.1"
                     /translation="MNIATNTGLRVVVLGYVVRGPLGGLAWHHLQYVTGLRALGHDVY
                     FVEDSDDYPSCYDPARGTVDADPSYGLRFAADAFARLGLGERWCYYDAHAARWAGPLA
                     PRAREVCESAELLLNLSGVNPLRPWTERTAARAFVDTDPAFTQIRHLTDPSARARAAA
                     HNSFFTFAENVARADCLVPPDGFAWQPTRQPVHLDAWPVTPGPRDGSWTTVMQWDSYR
                     AREYEGRRYGMKSDSFAAFVDLPARAGRVFEIALGSESAPRAHLRAKGWRLLDPLEVT
                     RDPWSYQEFIRGSKGEWSVAKHGYVASRSGWFSERSAAYLASGRPVVTQETGFSDWLE
                     TGRGLVAFDTPDGALAGMADIAGDYPRHCRAARALAEEFFDARKVLSSLIVRAHDGGA
                     AGGGLQGSVGGD"
     gene            complement(5500..9315)
                     /locus_tag="VF240_04115"
     CDS             complement(5500..9315)
                     /locus_tag="VF240_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7174453.1"
                     /translation="MRAFTSRRCLPAAFRLALSAAFALLCTGTAFAQSQSNAADLRGF
                     VRDQQGAVVNGATVTARQSATNTERNTTTNDEGFYQITNLPPGEYEVTIEAANFKRSV
                     NPTVTLTVGQQADLDFALEVGAVTESITVTTETEVVETSRTAVSNTIDETRIDNLPIN
                     QRDYINFTLTTSAVTRDNGRPIGPAPTSGLNFGGQRGRSNLVQVDGADNTDNSVNASR
                     STVSQEAVQEFQVVTNSYAPEFGRTSGGVVNVVTKSGTNDVRGNVFGFLRHKSIQARN
                     PFAPIIDGDPNKKPPFTRAQYGVTLGGPFDRDRTFFFGAFEQRRRQESGFFNSDVGAG
                     LTNTVTIGAPFLPFTQNFNGLSAAQATYTQGLLTQAGQAFAAGQTATGTALAGAAVQY
                     ATIASSGTNTALTGTNPLRSPGGAIPAGSVIGPRFFLSGAPVISSDIGFRPLNSLQRI
                     FPIAEKTTFSSFRLDHRITDKHQLTGRFSYNPSDISGIQVESQNQSLGQNDFSRTGIQ
                     TLRDTSFVTSLTSTLSNTVVNEARFNFGRRNATFRSQNGEAVAFNITGTAFIGRELFS
                     PVVRTETRYQWTDTINWVTGNHAFKFGGDVNFVAIPEALFELNFAGLFNFGGLTANTL
                     AAFPGNAPSFTPVQQYGLGFPGSFIQGYGNPISKLKNRPLAAFAQDSWKIWPNFTLNY
                     GVRYDIELTEQLDPVPFTDPLSGITLSANDILAAQDVLGVQQGFPRDKNNIAPRIGMA
                     WDLGNDGKTVVRAAFGIFYDHPLLAAAFNSDIADAAQQQQSTLLQGSPSPTSLLNAVQ
                     VFQGTVCVPGAVRTPVCAGLPAGVSTPGVAPTAEYQFGRLRFNDQTFPGFGQLLPFTL
                     PVAKDFVYAYANQGNLTVERRLSENMSLSASYLFVGAHHLPLPRDVNEIRQDLLIQNF
                     NRYVPNRGPISFGDAVLASTGLFPTTLAPGTAFANPLVAGDTLVVVVPGLIVASPTRG
                     RIVNPFAANFFRPSGPNYFLVRALTGLSPAAFNSLLAGSLATPTTVVSPFGDVNAQSS
                     IGNSNYHAGNFEFKRRFSDNYQFLASYTWSHSIDDSSDLQTLLKPQNNRNLAGERADS
                     LFDQRHRFVFSGVLTSPTSWRSGSGLHRFLADFVVAPIFEISSGRPFNILSGTDTNFD
                     LQGTNERPSVTADGTLCVPGPTSDVPECRGPLPANGSLGRNRGITHSYMSLDLRVARN
                     IHFGEHMRLEIIAEGFNLFNRFNEAAASPFFEDVRAFNQRDSRGRYFGRPTASFDPRQ
                     FQFGLKLNF"
     gene            complement(9889..11097)
                     /locus_tag="VF240_04120"
     CDS             complement(9889..11097)
                     /locus_tag="VF240_04120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7174454.1"
                     /translation="MSNETYDVLIVGGGPAGASAGIHLAARGARVLLAERERFPRPKL
                     CGEFISPECLAHFARLGVAERMTAAGGAAVGETIFYTRSGRSAAVPSAWLGREEQRAL
                     GLSRAEMDAQLLARAREVGVEVLEGAQAVGLVMEDGGRVCGVRLNVEGETVERRAALT
                     VDATGRAHALVRFVERGSVEGGAAARRGRAPHVAFKAHLEGARARVGACEIYFYRGGY
                     GGLSPVEGDLSNLCFIARAADVRDCGSDPERVMREVLMTNRRAARALEGARPASAWLS
                     VALEGFGRREPAPARGLLAVGDAASFIDPFTGSGMLMALESGELAAEAITRRLGRGDV
                     RAGHQKVFDELAGDYRMRYEERFGARLRLCGWLRRAAFAPRAFAEMAVFALGASAPAR
                     RRIARATRGT"
     gene            complement(11094..11807)
                     /locus_tag="VF240_04125"
     CDS             complement(11094..11807)
                     /locus_tag="VF240_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7174455.1"
                     /translation="MFPNFHERSNELERLDTGDYTSEEYEDCLVELRRVNRWLGDAHA
                     LRRSVLAAIDRDGLTEFSLLDVGAGSGELLRATSLWARGEKRVARLVGLELNARAARA
                     VLEESAEFKEIRAVRADALRMPFADGAFDYVMCSLFTHHFRDEALIALLREMSRVARR
                     RVFVIDLHRHRVAHLFYTTIARLFLRSPLVRADGALSILRSFVPAELRRLAERAGLSE
                     VEVRRRFPYRLVLSASAKH"
BASE COUNT         2069 a         3947 c         4122 g         1978 t
ORIGIN      
        1 gaacgagtgg atgcacctgc tgctcgaagc ggcccgagac gtttcgcgga cgattcccga
       61 gggggagact ttcatcctcg ttgacgaaca gggcttcggc caggacttcg cgcgggggcg
      121 gcgcgtgctt cccttcctcg aacgcgacgg agtctactgg ggcgcgccgc ccgacggcgc
      181 gacggccgtg cgcgagcttg agcggatgcg gcgcgagcgc ggcgcgggct tcatcgcctt
      241 cggctggccc tccttctggt ggctcgaaca ttacgcggcg ctcgccgcgc acctgcgcgc
      301 gcgctacgag tgcgtgcttg agaaccggcg gctcgtcgtc ttcgacctgc acgaccgagg
      361 aattaacaaa tgacgcgtcc gaagccgctc ttcatcatgg gcaataagcg ttccggcagc
      421 accctgatga ctgacctgct caactctcac gcgaacgtct tcgtgtcgca cgagtccgac
      481 atcgcctgga ttctatacca gtcgcggaac gggcggccca cccgctacca gacacacccc
      541 ctggacagca ggctgatgct cgactccacg ctggagagtt gccggcgaat cctgaaagag
      601 accctgagcg gcgagccgac gcacgccgaa atcgacgagg cgttctaccg cgcgcaggac
      661 cgcctcatga accaatacct gcggccgtcc ctcaagcagc ggctgaagcg cgccgccaag
      721 attaccggca agaggccgac gccggggcgc gtgctgcgcg cgctcagaca tgacccggaa
      781 ctcctacaga aaatagacct cgcctggacg ggcgacaaga agcacgccca acacctcgac
      841 ccggccgtcc ggcagttcct tcagacgcac ttcccggagg cgcgctacat ccacgtcatc
      901 aggcacccgc gcagcgtcgt ggcgagcacg atggaggcgg cccggcggtg gagcgagagg
      961 ccggagtatt tcaacggcct cgccgaacag attctcgaac agtgggccgt ccacgaggag
     1021 tgggtgcttg aggcgaagga gcgtgaggcg agtccgatcc tgacggtgag gcttgaagac
     1081 ttgtgggcgg agccggtcga aaccatgtcc gggattctgt gcttcctcga cctcgagatg
     1141 accgacgcct tcgccggcct ggtcaaacag atcgtctacc ccaaagaccc caacatcaag
     1201 tacgcctcgt tcccgctgcc ggacgttccg cgggcgcggc gcattatgga gctgtacagc
     1261 tactaaggcc ggggcggtac agcggaaaag gaaacagact gatgagtgtg agcgataatg
     1321 aggatatctt gatcgggccg tacggcggtc ggctcgtgag cctgctcgtc cccgaaggag
     1381 agcgcgaagc cgtgcgagag cgcgccgccg ccgccctgcc gcgcgtccaa ctcacgcccc
     1441 ggagcgtctg cgaccttgag ctgctcgcca ccggcgcctt ctcgccgctc ggcggcttca
     1501 tggggcgcgc agattacgag cgcgtgctcg aagagatgcg gctcgcctcc ggcgcgcttt
     1561 tcccgctgcc cgtcaccttg cccgtcgcgg cagacgcgaa cgtcaaagtt gatgaagagg
     1621 tcgccctcgt tgaccagcac aacaacctgc tcgccgtcat gcgcgtcgaa gaggtcttcg
     1681 agcgggagga cgaacgcgaa gccgcgctcg cctgcggcac acgagacgcg cgccacccgc
     1741 tcgtggccga gatgaactcg tggggccgcc tctacgtcgc cggccgtccg cgcgtccttg
     1801 agcttccccg ctactacgac ttccgcaatc tgcgtctgac gcccgccgaa gtgaggcgca
     1861 agctctcgtc gctcggtcac gaaaacgtcg tcgccttcca gacgcgcaac cccctgcacc
     1921 gcgcgcacga ggagttgacg cgccgcgccg ccgaggccgc ccgtggctca ctcctcctgc
     1981 acccagtcgt cggcctcacc aagcccggcg acatagatca cttcacgcgt gtccgctcgt
     2041 acaaggttct ggccgagcgt tattacgacc cgtcgcggat gctcttggcg ctgctgccgc
     2101 tggcgatgcg gatggccggc ccgcgcgagg ccctctggca cgcaatcatc cgaaggaact
     2161 tcggcgccaa tcatctcatc gtcggccgcg accacgcgag ccccggcctc gactcccgag
     2221 gccgtccgtt ctacggcccc tacgacgcgc aggaactcgt cgcgcggcac gaggcggaga
     2281 ccggcgtgcg cgccgtgccc ttcaacgaac tcgtctacct gcccgaggaa gaccgctacg
     2341 aggaggcttc gcgcatcccc gcgggccgcc gcaccgcctc gatttccgga accgaggtgc
     2401 gcgaagagta cctgcgccgc ggccgtcagc tcccgcgctg gttcacgcgc cctgaggtgg
     2461 cttcgatcct cacggaggcg cacccgccgc gccaccggca gggcttctgc ctctggttca
     2521 cggggctgtc cggcgcgggc aaatcgacga ccgccaacat cctcacagtg aaactcctcg
     2581 aacacggccg gcaggtgacc gtgctcgacg gcgacgtggt gcgcacgcac ctctcaaagg
     2641 ggcttggctt ctcgaaggaa gaccgtgaca cgaacatccg ccgcatcggc ttcgtcgcct
     2701 cggagatcgt caggcacggc ggcggcgtca tctgcgcggc cgtcagcccc tatcgcgcga
     2761 cccgcaacga gtgccgcgcg atggtcgcgg ccgaccgctt catcgaggtc ttcgtggaca
     2821 cgccgctcga agtctgcgag gagcgcgacg tgaagggcat gtacgccctc gcgcgcgagg
     2881 gcaagatcaa gaacttcact ggcgttgacg acccctacga gccgccgctc aaccctgaaa
     2941 ttgtcatcga cactgtcagg cgctcggccg aagagaacgc cgaactcatc ctcgcctacc
     3001 tcgtctcgca gggtctcgtc ctctcctccg ccgcgcaggc cgaggaggat gaatccgaga
     3061 agctggcggc gacaaagtga ccgagctgtt agctattagc tcttagcctt tagctcacag
     3121 catccgccgt cgggtgtgct gacgcaaaat tctaaagtac acggctgctt tcgttcggct
     3181 gacagctaac agctaatagc taacaggttc tttatgctct tcaccgaccg attcgtctac
     3241 gtccacgggc cgaagaccgg ggggacgttc gtgacctcga tgctgttccg gctctacggc
     3301 ctgcgctgga cgcgctgggc gcacgcgcgc aacgccctct tcggcgaggt gcgccgccgg
     3361 cacccgggct acggggcgct cgtccacaac agcaacaagc atggcggctg caacgaaatc
     3421 cccccgcccc aacgcggcaa gagaatcctc gccaccgtgc gcaacccttt cgacctctac
     3481 gtctcgcagt acgagttcgg ctggtggcgg cgggccgagt tccttcgcta ttacgaggcg
     3541 cttccacgtt tccgcgacga gtacccaagc ttccccgacc tgtccttcgc cgagtacgtc
     3601 caactttcga acgccgcgct cagtccgctc tcaggcggcg tgcgcgccga cggcgacgcc
     3661 cggccggggc tcatcaccga gcagttccta aacttctgct tccgcgaccc gcgcgcggcc
     3721 tacgagcggc tgcgcgcgcg ccgttacgcc gctcccaaag acgcggcggc cgacatgcat
     3781 gacatccact tcgtccgcac tgaccaactc aaccgcggtc tctatgactt cctcgtcagc
     3841 gagggctacg acccgggtga cgtgtccttc gtcctcgacg aagggaaaat cctcccgcgc
     3901 ggtcgcggcc ggggcgcgac gcagaagtgg cagcactact acacgcccga gctgaaggcc
     3961 cgggtcagag agcgggaaga tttcctcttc agactcttcc ccgagtttga cgtttgaggt
     4021 gccggccgcg cccccctcaa tgaacatcgc aacgaacacc ggcctgcgcg tcgtcgtcct
     4081 cggctacgtc gtgcgcgggc cgctcggggg gctggcctgg catcacctgc aatacgtcac
     4141 ggggctgcgc gccctcggcc acgacgtcta cttcgtcgag gacagcgacg actacccctc
     4201 ctgctacgac cccgcgcgcg gcacggttga cgccgaccct tcctacggac ttcggttcgc
     4261 tgccgacgcc ttcgcgcgcc tcggcctcgg cgaacgctgg tgctactacg acgcgcacgc
     4321 cgcgcgctgg gcggggccgc tcgcaccgcg cgcccgtgag gtctgcgagt cggcggagtt
     4381 actgctgaac ctgtcgggcg ttaaccccct gcgcccatgg acggagcgca cggcggcccg
     4441 cgccttcgtt gacaccgacc ccgccttcac acagatacgt cacctcaccg acccgtccgc
     4501 gcgcgcccgc gccgccgcac acaactcctt cttcacattc gctgaaaacg tcgcgcgcgc
     4561 cgactgcctc gtcccccccg acggcttcgc gtggcagccg acgcgccagc ccgtccacct
     4621 cgacgcgtgg cccgtcacgc cggggccgcg cgacgggagt tggacgaccg tcatgcagtg
     4681 ggacagctat cgcgcgcgcg agtatgaggg gcgccgttac ggaatgaagt ccgactcgtt
     4741 cgccgccttc gttgacttgc ccgcgcgcgc ggggcgggtc ttcgagatag ctttggggag
     4801 cgagtcggcg ccgcgcgcac acttgcgcgc gaagggctgg cggctgctcg acccgctcga
     4861 agtgacgcgc gacccctggt cttatcagga attcatccgc ggctcgaagg gcgagtggag
     4921 cgtcgccaag catggctacg tcgcgagccg ctcgggctgg ttcagcgagc gcagcgccgc
     4981 ctacctcgcc tcgggccgcc ccgtcgtcac gcaagagacg ggcttctccg actggcttga
     5041 gacgggccga gggctagtcg ccttcgacac gcccgacggc gcgctcgccg gcatggcaga
     5101 catcgccggg gactacccgc gccactgccg cgccgcgcgc gctttggccg aggagttctt
     5161 cgacgctcgc aaagtcttga gtagcctgat tgtgcgtgcc catgatggcg gagcggctgg
     5221 aggcgggctg caaggctctg tcggaggaga ttagagctta cgagggcgtg gccgaccagc
     5281 taagcgctaa gctcgcggcc gaagagtgca ccaaagatat tgcgcgtcag ttcgcggact
     5341 actgccggaa gcttgacggc gcgttcgaga cggtgagcat ccccttcgag aagaagcggg
     5401 agatgctcac cgtcttgcgt gtgactgtgc tcgctcattc gaacggcacg ctgaagttgc
     5461 aaacaaatct cggagcgatt ttagctatct gccctgaaat caaaagttta gtttcaagcc
     5521 aaattggaac tgacgcgggt cgaaggaggc ggtggggcgg ccgaagtagc ggccgcggct
     5581 gtcgcgctgg ttgaaggcgc gaacgtcttc gaagaagggc gaggccgccg cttcgttgaa
     5641 gcggttgaag aggttgaagc cctcggcgat gatttcgaga cgcatgtgct cgccgaagtg
     5701 gatgttgcgc gccacgcgca ggtcgagcga catgtaggag tgcgtgatgc cgcggttgcg
     5761 acccagactg ccgttggccg gcagcggccc gcggcactcg ggtacgtccg aggtcggccc
     5821 cggtacgcag agcgtgccgt cggccgtcac gctcgggcgc tcgttcgtgc cctgaaggtc
     5881 gaagttcgtg tcggtgccgc tcaggatgtt gaaggggcgg cccgaggaga tttcaaagat
     5941 cggggcgacg acgaagtccg cgaggaaacg atgtaagccg gagccggagc gccagctcgt
     6001 ggggcttgtg agcacgccgc tgaagacgaa gcggtgacgc tggtcgaaga gcgagtcggc
     6061 gcgctcgccg gcgaggttgc ggttgttctg cggcttgagg agcgtctgga ggtcggagga
     6121 gtcgtcgatg gagtgcgacc acgtgtagga ggcgaggaac tggtagttgt cggagaagcg
     6181 gcgcttaaac tcgaagttgc ccgcgtggta gttggagttg ccgatggagg actgggcgtt
     6241 cacgtcgccg aagggcgaga cgaccgtcgt gggcgtggcc agcgagccgg cgagcaggct
     6301 gttgaaggcc gcgggcgaca gacccgtgag cgcgcgcacg aggaagtagt tcggccccga
     6361 cggacggaag aagttcgcgg cgaacggatt gacgatgcgc ccgcgcgtgg gcgaggccac
     6421 gatgagaccg ggcacgacga cgacgagcgt gtcgccggcg acgagcgggt tggcgaaggc
     6481 ggtgcccggc gccagtgtcg tcgggaagag gccggtggag gcgagcacgg cgtcgccgaa
     6541 gctgatcggc ccgcggttgg ggacgtagcg gttgaagttc tgaatgagca ggtcttgtcg
     6601 aatctcgttc acgtcgcgcg gcagcggcag gtggtgcgcg ccgacgaaga gataagaggc
     6661 tgagagcgac atgttctccg aaagccggcg ctcgaccgtc aggttcccct gattggcgta
     6721 agcgtagacg aagtctttcg ccacggggag cgtgaagggg aggagctggc cgaagccggg
     6781 gaacgtctgg tcgttgaagc gcaggcggcc gaactgatac tcggccgtgg gcgccacacc
     6841 cggcgtggag acgcccgccg gcagacccgc gcagacgggc gtgcgtaccg caccggggac
     6901 gcagaccgtg ccctgaaaga cctgcacggc gttcaagagc gaggtcgggc tggggctgcc
     6961 ctggagcaag gttgactgct gctgctgcgc ggcgtcggcg atgtcggagt tgaaggcggc
     7021 ggcgagcagc gggtggtcgt agaagatgcc gaaggcggcg cgcacgaccg tcttgccgtc
     7081 gttgcccaaa tcccacgcca tgccgatgcg gggcgcgatg ttgttcttgt cgcgcgggaa
     7141 gccctgctgg acgcctaaca cgtcctgcgc ggcgaggatg tcgttggccg agagcgtgat
     7201 gccggagagc gggtcggtga agggcacggg gtcgagctgc tcggtcaact caatgtcgta
     7261 gcggacgccg tagttgagcg tgaagttcgg ccaaatcttc caggagtcct gcgcgaaggc
     7321 ggcgagcggc cgattcttga gcttgctgat ggggttgccg tagccctgga taaagctgcc
     7381 ggggaagccg aggccgtact gctgcacggg ggtgaaggag ggcgcgttgc cggggaaggc
     7441 ggcgagcgtg ttggcggtca gaccgccaaa gttgaagagg ccggcgaagt tcagctcgaa
     7501 gagggcctcg gggatggcga cgaagttgac gtcgccgccg aacttgaagg cgtggttgcc
     7561 ggtgacccag ttgatggtgt cagtccactg gtagcgcgtc tcagtgcgaa cgacgggcga
     7621 gaacagctcg cggccgatga aggccgtgcc cgtgatgttg aaggcgacgg cctcgccgtt
     7681 ctgcgagcgg aaggtggcgt tgcggcgtcc gaagttgaag cgcgcctcgt tgaccacggt
     7741 gttcgagagc gtcgaggtga gcgaggtgac gaacgaggtg tcgcgcaggg tctggatgcc
     7801 ggtgcgcgag aagtcgttct gtccgagcga ctggttctgc gactcgacct gaatgccgga
     7861 gatgtctgac gggttgtagc tgaagcggcc cgtgagctga tgtttgtcgg tgatgcggtg
     7921 gtcgaggcgg aacgaggaga aggtcgtctt ctcggcgatg gggaagatgc gttgcaggga
     7981 gttgagcggg cggaagccga tgtcgctgga gatgacgggc gcgcccgaca ggaagaagcg
     8041 cgggccgatg acgctgcccg cggggatcgc gccgccgggg ctgcgcagcg ggttggtgcc
     8101 ggtgagcgcc gtgttcgtgc cgctcgaagc gatggtcgcg tactggacgg cggcgcccgc
     8161 gagcgccgtg cccgtggcgg tctgtccggc agcgaaagcc tgccccgcct gcgtgagcaa
     8221 gccctgcgtg taggtggcct gcgccgcgct caggccgttg aaattttgcg tgaagggcag
     8281 gaagggcgcg ccgatggtca cggtgttcgt gaggccggcg ccgacgtcgg agttgaagaa
     8341 gcccgactcc tgccggcggc gctgctcgaa ggcgccgaag aagaaggtgc ggtcgcggtc
     8401 gaaggggccg cccaaggtga cgccgtactg cgcgcgcgtg aagggcggct tcttgttggg
     8461 gtcgccgtcg atgatgggcg caaacgggtt gcgcgcctga atcgacttgt gtcggaggaa
     8521 gccgaagacg ttgccgcgca cgtcgttggt gcccgacttg gtgacgacgt tgacgacgcc
     8581 gcccgacgtg cgcccgaact ccggcgcgta ggagttggtg acgacctgaa actcctgcac
     8641 ggcctcctgc gagacggtcg agcgcgaggc gttgacggag ttgtctgtgt tgtcggcgcc
     8701 gtcaacctgc acgaggttgg agcggccgcg ctggccgccg aagttaaggc ccgaggtggg
     8761 cgccggcccg atggggcggc cgttgtcgcg cgtcacggcc gaggtcgtca gcgtgaagtt
     8821 gatgtaatcg cgctggttga tggggaggtt gtcgatgcgc gtctcgtcga tggtgttgga
     8881 gacggccgtg cgcgaggtct cgacaacttc cgtctcggtc gtcaccgtga tcgactcggt
     8941 gacggcgccg acttcgagcg cgaagtcgag gtcggcctgc tggccgacgg tgagcgtcac
     9001 cgtggggttg accgaccttt tgaagttggc ggcctcaatc gtcacctcat attcgcccgg
     9061 cggcaggttc gtgatctggt agaagccctc gtcgttggtc gtggtgttgc gctcggtgtt
     9121 ggtggcgctc tggcgcgccg tcaccgtggc cccgttgacg accgcgccct gctggtcgcg
     9181 cacgaagccg cgcaggtcgg cggcgttcga ctgtgactgg gcgaaggccg tgcccgtaca
     9241 aagaagcgca aacgctgcgc tgagcgccag acggaaggcg gccgggagac accgccgaga
     9301 ggtgaacgca cgcatgtttt tcattctcct tgaaacaaag gtcgaagctt taatcacgaa
     9361 cgaggcgaga cgcgcgccct cgcgcacgaa gcacgccacg agtgccggcc gtcctccgct
     9421 ggaaaggctt cggaagactg cgcgggtgaa gcaaactgcg gcgcgcatcg ggagcacggt
     9481 caaattttac gcgcaagaag gaggctcaaa ggttattgct tccgggccgg acgcatgcgc
     9541 ctcggcgggc gcgtccggcg gcaatcttct aggccacgct tatttacagc aaaaccggtc
     9601 tgttggcaag aaccttgtcg cgccagccgc gcgcgtagtt tcaaaacctc gacgccggtc
     9661 atgtccgtag gggcagggct tgtccgcggc ccgctcaacg atggaagcaa ttaacatgga
     9721 tggacaggat taacaggatg agatgatttg aaatctcaaa tctccaatct ccaatctgaa
     9781 atctcaaatc agcgctttct tcatcctccc tatcctgtcc atccatgtta attcctctgt
     9841 agctaagggc cgcggacaat ccctgcctct acagtttcgc aacgcgccct aagttccgcg
     9901 tgtcgcgcgg gcgatacggc ggcgcgccgg agcgctcgcg ccgagcgcga agacggccat
     9961 ctcggcgaac gcgcgtgggg cgaaggccgc ccggcgcagc cagccgcaca gacgcagacg
    10021 cgccccgaaa cgctcctcgt agcgcatgcg gtagtcgccg gccaactcgt cgaagacctt
    10081 ctggtgcccg gccctcacgt cgccgcggcc gagccggcgc gtgatggctt cggccgcgag
    10141 ttcgccgctc tccaaagcca tcagcattcc gctcccggtg aacgggtcga tgaaggaggc
    10201 ggcgtcgccg acggcgagca gcccccgcgc cggcgccggc tcgcgccgtc cgaagccttc
    10261 gagcgcgacg ctgagccacg cggaagccgg gcgcgcgcct tcgagcgcgc gtgcggcgcg
    10321 gcggttcgtc atcagcacct cgcgcatgac gcgctcgggg tcgctcccac agtcgcgcac
    10381 gtcggcggcg cgcgcgatga aacagaggtt gctcaaatcc ccctcgaccg gactcaggcc
    10441 gccgtagccg ccgcggtaga agtagatttc gcacgcgccc acacgggcgc gtgcgccctc
    10501 cagatgggcc ttgaaagcga cgtgcggcgc gcgcccgcgc ctcgcagccg cgccgccctc
    10561 aacacttccc ctctcgacga agcgcacgag cgcgtgcgcc ctgcccgtcg cgtcaacggt
    10621 caacgccgcg cgccgctcga ccgtctcgcc ttcgacgttc agacgcactc cgcacacgcg
    10681 ccctccgtct tccatcacca gaccgacggc ctgcgctccc tccaaaactt cgacacccac
    10741 ttctctcgca cgcgcgagta gttgcgcgtc catctcggcg cggctcagac cgagcgcacg
    10801 ctgttcctca cgaccgagcc acgcgctcgg cacggccgcg ctccggccag agcgcgtgta
    10861 gaaaatcgtc tcgccgacgg cggcgccgcc cgcggccgtc atccgctccg ccacgccgag
    10921 ccgcgcgaag tgtgcgaggc attccggcga gatgaactcg ccgcacagct tcgggcgcgg
    10981 gaaccgctcg cgctcggcca gaagcacacg cgcgccgcgc gccgccagat gtatcccggc
    11041 gctcgccccc gccggcccac cgccgacgat caacacgtca taagtttcat tactcaatgc
    11101 ttcgcgctcg cgcttagaac cagtcggtaa ggaaaacgcc gtctcacctc cacctccgac
    11161 agacccgcgc gctcggccag ccggcgcagc tccgcgggga cgaagcttcg caggatggag
    11221 agcgcgccgt ccgcgcgcac gagcgggctg cggaggaaga ggcgggcgat tgtcgtgtag
    11281 aagaggtgtg cgacgcggtg ccggtgcaga tcgatgacga agacccggcg tcgagcaacg
    11341 cgcgacatct cgcgcaggag cgcgattaac gcctcgtcgc gaaagtggtg tgtgaagagc
    11401 gagcacatca cgtagtcgaa cgcgccgtcg gcgaatggca tccgcaaagc gtcggcgcgc
    11461 acggcgcgaa tctctttgaa ctccgcggac tcttcaagga cggcgcgggc cgcgcgcgcg
    11521 ttcaactcaa ggccaacgag ccgcgcaaca cgtttctctc ccctcgccca caggctggtt
    11581 gcgcgcagca attcgcccga gcctgcgccc acgtcgagca acgaaaactc cgtcaggccg
    11641 tcgcggtcga tggcggcgag tacggagcga cggagcgcgt gcgcgtcgcc gagccagcgg
    11701 ttgacgcggc gcagctcgac gagacagtcc tcgtattcct cgctcgtata gtcgcccgtg
    11761 tcgagtcgct caagctcgtt gcttcgctcg tggaagttgg ggaacatctg ggacggccgc
    11821 gttcttcgtt gttgagtgtg acgaaaggta actcaccgca gaggcgcgga ggacgcggag
    11881 ggggcgcaga gagattcaac gctccgcgta atctctgcgt cctccgcgcc tctgcggtga
    11941 gtcgccgtcg cccagacact caagactatt tccaccctca agatcggaca cgcgtctcaa
    12001 atcttctcca gcacgagcgc gctgttcttc gagccgaagc cgatgcagtt gcagagcgcg
    12061 acgcgcacgc gcccctcgcg tgcgcggttg ggcacgtagt cgaggtcgca cgcggg
//
LOCUS       DASVES010000112         6769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_641287, whole genome shotgun sequence.
ACCESSION   DASVES010000112 DASVES010000000
VERSION     DASVES010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641287"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VF240_04130"
     CDS             complement(<1..792)
                     /locus_tag="VF240_04130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEX7174456.1"
                     /translation="MTIHEKMFIGAIGCEPLQLRRVGLKDSHTLAVYRQNGGYEALKT
                     VLTGMSPDDVIAELKKSALRGRGGAGFPTGMKWSFVPKDSPKPKYVVCNADESEPGTF
                     KDRYLMERDPHMLIEGMLIAGYALGSKTGYVYTRGEYRYLINIMDAAIKEAYREGLLG
                     QKILGADFQFDLYTHTGAGAYICGEETALLNSLEGLRGHPRMKPPFPAVAGLYGCPTV
                     VNNVETLTAVPDIIKMGGEAWQKLGTEKSGGTKVWSVSGHVNRPGV"
     gene            complement(852..1418)
                     /gene="nuoE"
                     /locus_tag="VF240_04135"
     CDS             complement(852..1418)
                     /gene="nuoE"
                     /locus_tag="VF240_04135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7174457.1"
                     /translation="MSTQFTPNNPLPQEAKLPDEVAKFSAEVEAEIERHLAKYPVRRS
                     AILPLMFIVQRERGGHLDPPGVAYLADRLGVRVTDIWEVATFYSMIYTEPVGKYHIQV
                     CRTLSCKIMGAGKVAEQCAKRLGIKPGETTADGRFSLSEVECLGSCGTAPMLQLNLDY
                     HENLTDEKVDALLDECERGQYEFKVKVS"
     gene            complement(1510..2730)
                     /gene="nuoD"
                     /locus_tag="VF240_04140"
     CDS             complement(1510..2730)
                     /gene="nuoD"
                     /locus_tag="VF240_04140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX7174458.1"
                     /translation="MSATTNILPPPFEVYDQSLDTQMTISFGPQHPSTHGVLRLELTL
                     DGEMIVKCVPDIGYLHTGMEKLFEYKKYQQGIVITDRMDYLNPLGNNLAYVMAVEKLL
                     GLEIPERAQVIRVLMCELQRIASHMVWLGTHALDIGAMSIFFYAFREREKILNLIEAA
                     SGGRLTPSYFRIGGLMMDLPAGFERRARQFTGKFIHAVDEFHTLLSGNTIFQKRTQGV
                     GRISAEDAIDWGLTGPSLRGSGVALDLRKVNPYTGYETYDFDVPVEPDGDVWARYMVR
                     MRELKESWKIVEQALDRLKPGPVKADAPKVVLPEREAMKTHMDALIHHFLIVAEGFNV
                     PAGEVYHPIEGSKGELGCYVISDGGPKPARVHFRGPSFVNLSALPHMSEGEMIADVVA
                     IIGSIDIVLGDIDR"
     gene            complement(2862..3407)
                     /locus_tag="VF240_04145"
     CDS             complement(2862..3407)
                     /locus_tag="VF240_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7174459.1"
                     /translation="MDIEREKQIAEGARSSTGLSYPLPTVEALRALQTEHAGWVSDII
                     EAFGELTAVVPRAGIAEICAHLKTAPGMEFNLLADICGADRGPEEEPRYEVNYHLFST
                     TRFHRLRLKVFLNDEDTHVPTVTGVWRTANWHERETFDMFGVIFDGHPDLRRILLPDD
                     WQGHALRKDFPLRGYEPYSLT"
     gene            complement(3490..4125)
                     /locus_tag="VF240_04150"
     CDS             complement(3490..4125)
                     /locus_tag="VF240_04150"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX7174460.1"
                     /translation="MGLETILSEALPEIITTRLDSLVNWARKSSLWPATFGLACCAIE
                     MMNATSARNDLARFGSEVFRASPRQADVMIVSGRVSRKMAPVLRRIYDQMPEPKWVIS
                     MGACATSGGVFDNYAIVQGVDKIVPVDVYIPGCPPRPEMLIHAVMMLQDKVMKESLRD
                     RRDVRDEDSREAVPPGTLPASGVSATNEAAAEGAPSRPYTIASRHERRRSS"
     gene            complement(4143..4616)
                     /gene="ndhC"
                     /locus_tag="VF240_04155"
     CDS             complement(4143..4616)
                     /gene="ndhC"
                     /locus_tag="VF240_04155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX7174461.1"
                     /translation="MFNLSDYLPILLMFGVAIGFAVGNVLLSQFVGQRKRTRTKSMPY
                     ECGKDPVGSARERFSVKFYLIAMIFILFDIELIFLMPWAVVFKKLTLEDGLGTIAYVE
                     MMLFVALLLVGYVYVLKKGLFEWGERSRVEAEAEARAIHVADDARRKQPRVVRAA"
     gene            4877..5776
                     /locus_tag="VF240_04160"
     CDS             4877..5776
                     /locus_tag="VF240_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174462.1"
                     /translation="MITGFNTDIEHGGVTYHVQTEDKGLENPLILSLVYVGGAILASK
                     RTTYEDLIEKGFDEKALAERLQRQHKLLCAAVRAGRVDDLKRMGGERDAAPAASPAPP
                     AINEAAPINGQALKSSPPEAKTEKPAAAPKPPAAIPILDLITDAATPIIPEGIKLPPP
                     PPAFNAYQTAATVDEDEPEQLDADAGVIPVEVYDEDADELYLKLLDDEGEFRAGQLAT
                     IKIYVGRGKFGRQSVTDASVTVKVLGTTFRPLILNTRTDEEGVAVVRALLPQFNSGRA
                     ALLIRAADAHGAEAELRRIIHQG"
     gene            5773..5988
                     /gene="thiS"
                     /locus_tag="VF240_04165"
     CDS             5773..5988
                     /gene="thiS"
                     /locus_tag="VF240_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX7174463.1"
                     /translation="MKDNVLQVTVNEEAREVPAETTLAGLVEHLNLAPERLAVEHNGR
                     VVRRAEWPRIELSDGDRVEIVHFVGGG"
     gene            6135..>6769
                     /locus_tag="VF240_04170"
     CDS             6135..>6769
                     /locus_tag="VF240_04170"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX7174464.1"
                     /translation="MSATQKTETHSFEIGGKTFTSRLIVGTGKYRSFDEMKAAHRASG
                     AEMVTVAVRRVPLDRRSESFLDHLDPSMTILPNTAGCYTAEEAVRTARLAREALSTDL
                     IKLEVIGDQTTLFPDNEQTVEAARQLVREGFTVLPYFSDDLIVAKKLLDAGCAAVMPL
                     AAPIGSGLGVQNPANLLIMREQLPEALIIVDAGVGTASDAAVAMELGADAVL"
BASE COUNT         1276 a         2163 c         2139 g         1191 t
ORIGIN      
        1 gacgccgggg cggttgacgt ggcccgagac cgaccacacc ttcgtcccgc cgctcttctc
       61 cgtccccaac ttctgccacg cctcgccgcc catcttgatg atgtcgggga cggccgtcag
      121 cgtctcgacg ttgttgacca ctgtggggca gccgtagaga ccggcgactg ccgggaaggg
      181 cggcttcatg cgcgggtggc cgcgcaagcc ttcgagcgag ttcaaaagcg ccgtctcctc
      241 gccgcagatg tatgcgccgg cgccggtgtg tgtgtagaga tcaaactgga agtcagcgcc
      301 gagaatcttc tggccgagca agccctcgcg gtaagcctcc ttgatggccg cgtccatgat
      361 gttgatgagg tagcggtatt cgccgcgcgt gtagacgtag ccggtcttcg agccgagcgc
      421 gtagcccgca atcaacatgc cctcgatgag catgtgcggg tcgcgctcca tcagataacg
      481 atccttgaag gtgcccggct ccgactcgtc ggcgttgcag acgacgtact tcggtttcgg
      541 cgaatccttc gggacgaagc tccacttcat ccccgtgggg aagcccgcgc cgccgcggcc
      601 ccggagcgcc gacttcttca gctccgcgat cacgtcgtcg ggcgacatcc ccgtgaggac
      661 agtcttgagc gcctcgtagc cgccgttctg ccggtagacc gcgagcgtgt gcgagtcctt
      721 caggccgacg cgcctgagct ggagtggttc acaaccgatg gccccgatga acattttttc
      781 gtgaatcgtc aatcgcgagt cgtaaatcgt taaataactg tgcggcgctg gcgcaaagct
      841 tcggcgacgc cttaagagac tttgactttg aactcgtact ggccgcgctc gcactcgtcc
      901 aagagcgcgt caaccttctc gtcggtcagg ttctcgtggt agtcgaggtt gagttgcagc
      961 atcggcgccg tcccgcatga gccgagacat tcgacctcgg agagcgagaa gcggccgtcg
     1021 gcggtcgtct cgcccggctt gatgccgagc cgcttcgcac actgctcggc gactttcccc
     1081 gcgcccatga ttttgcacga gagcgtgcgg cagacctgga tgtgatactt gccgaccggc
     1141 tccgtgtaga tcatcgagta gaaggtggcg acctcccaga tgtccgtgac gcgcacgccc
     1201 agacggtcgg ccaagtaggc cacgcccggc gggtcgagat gtcccccgcg ctcgcgctgc
     1261 acgatgaaca tcagcggcaa aatggccgag cgcctcaccg gatacttcgc cagatgacgc
     1321 tcgatctcgg cctccacctc agcggagaac ttcgccacct cgtcaggcag cttcgcctcc
     1381 tgcggcagcg ggttgttggg cgtgaactgt gtgctcataa aatcgggagt caggagtcag
     1441 gagtcagtat acagaatgaa gaaggggcac gcctttcatt ctgtctcctg actcctgact
     1501 cctgacttct catctatcaa tgtcccccag cacgatgtca atcgagccga tgatggcgac
     1561 cacgtcggcg atcatttcgc cctcggacat gtggggcagc gccgagaggt tgacgaagga
     1621 ggggccgcgg aagtgtacgc gggcgggctt ggggccgccg tcggatatga cgtagcaacc
     1681 gagttcgccc ttcgacccct caatcgggtg atagacctcg ccggcgggga cgttgaagcc
     1741 ctcggcgaca atcaggaagt ggtggatgag cgcgtccatg tgcgtcttca tcgcctcgcg
     1801 ctcgggcaac acaaccttcg gcgcgtcggc cttcaccggc cccggcttca gacggtcgag
     1861 cgcctgctcg acgatcttcc aagattcctt tagctcgcgc atcctgacca tgtagcgcgc
     1921 ccacacgtcg ccgtccggct cgaccggcac gtcgaagtcg taggtctcgt agcccgtgta
     1981 agggttcact ttgcggaggt ctaaagcgac gccgctgccg cgaagcgacg gccctgtcag
     2041 gccccagtcg atggcgtcct cggccgagat gcggccgacg ccctgcgtgc gcttctggaa
     2101 aatcgtgttg cccgagagca gcgtgtggaa ctcgtcaacc gcgtggatga acttcccggt
     2161 gaactgccgc gcgcggcgct cgaagccggc gggcaagtcc atcatcaggc cgccgatgcg
     2221 gaagtacgac ggggtgagcc gcccgcccga ggcggcctcg atgaggttga gaatcttctc
     2281 gcgctcgcgg aaggcgtaga agaagatgga catcgcgccg atgtctaagg cgtgcgtccc
     2341 cagccagacc atgtgcgagg cgatgcgctg caactcgcac atcagcacgc ggatgacctg
     2401 cgcgcgctcc ggtatctcca ggcccagcag cttctcgacc gccatcacgt aggcgagatt
     2461 gttgccgagc gggttgagat agtccatccg gtcggtgatg acgatgccct gctggtactt
     2521 cttgtactcg aagagcttct ccatccccgt gtgcaggtag ccgatgtcgg gcacgcactt
     2581 gacgatcatc tcgccgtcga gagtcaactc cagcctcaac acgccgtgcg tggacgggtg
     2641 ctgcggcccg aacgagatgg tcatctgcgt gtcgagcgac tggtcgtaca cctcgaaggg
     2701 cggcggcagt atgttggtcg tagcactcat cttttgatag gtgataagtg ataagtgata
     2761 agtgataaga aagaaaggaa gctgttagct attagctgtt ggccgttagc taatcgctaa
     2821 aagctaacag cctcttatca cttatcactc atcactcatc actaagttaa gctgtacggc
     2881 tcgtaaccgc gcagcgggaa gtctttacgc agggcgtggc cctgccagtc gtcgggcaga
     2941 aggatgcggc gcaggtcggg gtgcccgtcg aagatgacgc cgaacatgtc gaaggtctcg
     3001 cgctcgtgcc agttcgcggt gcgccacaca cccgtcaccg tggggacgtg cgtgtcctcg
     3061 tcgttcagga aaaccttgag acgcaggcgg tgaaatctgg tcgtcgagaa aaggtgatag
     3121 ttgacctcgt agcgcggctc ctcctcgggg ccgcgatccg ccccgcagat gtcggcgagc
     3181 agattgaact ccatgccggg cgccgtcttc aggtgagcgc agatttcggc tatcccggcg
     3241 cgcggcacga cggccgtcaa ctcgccaaaa gcctcgatga tgtcgctcac ccagcccgcg
     3301 tgctccgtct gaagcgcacg caacgcttca accgtgggca ggggatacga cagccccgta
     3361 ctggagcgcg ccccctcggc gatctgcttc tcccgctcaa tgtccataaa agtaagcagt
     3421 aagcagtgag cagtgagcag taaacagcag aagcatttga ctgcttactg cttactgctc
     3481 actgctcact cacgaagatc tgcggcgctc gtgtcgcgac gcgatggtgt agggccgcga
     3541 gggcgctcct tcggcggcgg cttcgttggt ggccgacact cccgaagccg gcagtgtgcc
     3601 cggcgggacg gcctcgcgcg aatcctcgtc gcgcacgtcg cggcggtcgc gcagagactc
     3661 cttcatcacc ttgtcctgaa gcatcatcac cgcgtgaatc aacatctctg gtcgtggcgg
     3721 gcagcccgga atgtagacat cgacgggcac gatcttatcc acaccctgca cgatggcgta
     3781 gttgtcgaac acaccgcccg aggtggcgca cgcgcccatc gagatgaccc acttcggctc
     3841 gggcatctgg tcgtagatgc gccgcagcac gggcgccatc ttgcgcgaga cgcggcccga
     3901 gacgatcatc acgtcggcct gccgggggct ggcgcggaaa acttcggagc cgaaacgcgc
     3961 gaggtcgttg cgggccgagg tggcgttcat catctcgatg gcgcaacaag cgagtccgaa
     4021 ggtcgccggc cacaagctgg acttgcgcgc ccagttgacg agcgagtcga gccgcgtggt
     4081 gatgatctcg ggcagagcct cggaaagaat tgtctctaaa cccatcggta aaacctcccc
     4141 gatcaggcag cgcgcacaac acgcggctgc ttgcgccgcg cgtcgtccgc cacgtggatg
     4201 gcgcgcgcct cggcctcagc ctccacgcgc gaacgctcgc cccactcgaa cagtcccttc
     4261 ttcagaacgt agacgtagcc caccaggagc agcgcgacga agagcatcat ctcgacgtag
     4321 gcgattgtcc cgaggccgtc ttcaagcgtc agcttcttga agaccacggc ccacggcatc
     4381 aggaagatca gctcgatgtc gaagaggatg aagatcatcg cgatcaggta gaacttgacg
     4441 gagaaccgct cgcgtgcgga gccgacgggg tctttgccgc actcgtaagg catcgacttc
     4501 gtgcgcgtgc gcttgcgctg gccgacgaac tgcgagagca ggacgttgcc cacggcgaag
     4561 ccgatggcca cgccgaacat gagcaggatg ggcaggtagt cgctcaggtt gaacacactg
     4621 gcctcccttc ggccgcgggg ccgaagcgga cgtgccccgc ggccgccctc aactgagttt
     4681 gagaatcaag gtacaagcgg tatcgtagta gaaggtctgt cgggtgtcaa ggatgcgaga
     4741 ttggcgggaa aagactgtcc cgaaaggaga ttgaccgcgt cggaagccgt gtgctacttt
     4801 gattttcaag ccctttcgcg ggggcgcacg cccttcaaca tcccccttcg cagaaacaga
     4861 taatcggtcg tcggccgtga tcaccggatt caacacagac atcgaacacg gtggcgtcac
     4921 ctaccacgtc cagacggagg acaaggggct ggagaacccc ctcatccttt cgctcgtcta
     4981 cgtcggcggg gccatcctcg ccagcaagcg cacgacctac gaagacctca tcgaaaaggg
     5041 gttcgacgag aaggcgctcg ccgaacgact ccagcgacag cacaagctgc tgtgcgcggc
     5101 cgtgcgcgcc gggcgcgtag acgacctcaa gcgcatgggt ggcgagcgcg acgccgctcc
     5161 ggccgcttcg cccgcgccgc ccgcgatcaa tgaagcagcg cccatcaacg ggcaggcatt
     5221 gaagagttcg ccgccagagg cgaagacgga gaaacccgcc gcggcgccga agccgccggc
     5281 cgcgattccc atcctcgatc tgatcaccga cgcggccacg ccgatcatcc ccgaaggcat
     5341 taagcttccc ccgccgccgc cggcattcaa tgcgtatcag acggccgcga cggtcgatga
     5401 ggacgagccg gagcagttgg acgcggacgc gggcgtaatc ccggtcgagg tctacgacga
     5461 agacgcggac gagctgtatc tgaaactgct cgacgacgaa ggcgagttcc gcgcggggca
     5521 actcgcgacc atcaagattt acgtcgggcg cggaaagttc ggcaggcagt cggtgacgga
     5581 tgcgtctgtg accgtcaagg ttctgggaac gaccttccgc ccgctcatcc tcaacacgcg
     5641 gacggacgaa gagggcgtgg ccgtcgtgcg cgcactgctg ccgcagttca actcgggtcg
     5701 cgccgccctc ctcatccgcg ccgccgacgc gcacggcgcg gaggccgagt tgcgccggat
     5761 tattcatcag ggatgaaaga caacgtgttg caggtcacgg tcaacgagga ggcgcgcgaa
     5821 gtcccggcgg agacgacgct ggccggcctc gtcgaacatc tcaatctcgc ccccgagcgc
     5881 ctcgccgtgg agcacaacgg gcgggtcgtt cgccgcgccg agtggccgcg catcgaactc
     5941 tcagatggcg accgcgtcga gatcgtccac ttcgtcggcg gcggttgagt cgcttgttcc
     6001 gtgagattgt tcgtgagcgt cgcgcgggcc gtgaggagcg cgttttcgtt tcgggggagt
     6061 tagggtgtag atttatgcgg agagaagcag acacaagata ggccgccgac ttcgggcgca
     6121 tcctccgaaa tacaatgagc gcaacccaga aaacagagac gcattcgttc gagatcggcg
     6181 gcaagacttt cacctcacgt ctcatcgtcg gcacgggcaa gtatcgcagc ttcgacgaga
     6241 tgaaggcggc gcaccgggcc tcgggcgcgg agatggtgac ggtggccgtg cggcgcgtgc
     6301 cgctcgaccg ccgctccgag tcgttcctcg accacctcga cccgtcgatg acgatcctgc
     6361 ccaacacggc cgggtgctac acggccgagg aggccgtccg cacggcgcgg ctcgcgcgcg
     6421 aggcgctctc gaccgacctc atcaagctcg aagtcatcgg cgaccagacg acgctcttcc
     6481 ccgacaacga gcagacggtg gaggccgcac gccaactcgt gcgcgagggc ttcaccgtgc
     6541 tcccgtactt ctccgacgac ctcatcgtcg ccaaaaaact cctcgacgcc ggctgcgcgg
     6601 ccgtgatgcc gctggccgcc cccatcggct ccggcctcgg cgtgcagaac ccggcgaacc
     6661 tgctcatcat gcgcgagcaa ttgcccgaag cactcatcat cgtggacgcg ggcgtcggca
     6721 ccgccagcga cgcggccgtc gccatggaac tcggagccga cgccgtcct
//
LOCUS       DASVES010000113         5702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_619890, whole genome shotgun sequence.
ACCESSION   DASVES010000113 DASVES010000000
VERSION     DASVES010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619890"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF240_04175"
     CDS             <1..330
                     /locus_tag="VF240_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7174465.1"
                     /translation="GGEKKRNEILQMAVLEPKLAILDETDSGLDIDALRIVADGVNQL
                     RSPSNAIVLVTHYQRLLNYIVPDHVHVLFQGRIVRSGGRELALQLEEKGYDWIREEVT
                     TQQTAAG"
     gene            447..1781
                     /gene="sufD"
                     /locus_tag="VF240_04180"
     CDS             447..1781
                     /gene="sufD"
                     /locus_tag="VF240_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX7174466.1"
                     /translation="MSHAVIEKSSYVDAFRESRRLAGGSLPRWFDNLRGGSIERFEEV
                     GFPTTDEEDWKYTNVAPIARRVFHPASGQAEAAFDSSLIGPYAYQESKGSRVVFVDGI
                     YRADLSSLEGLPAGVEVSQLTDALEGEHSEVVRKHLEVAAAADSFAALNTALFDAGLL
                     LRIGRGVVVESPIQLLFLAASEREDTAAFPRVLIVSEPQSSATVIETYDAVGDQVYFT
                     DSVVEARVGDGARLVHYRVQREGERAFHVASTRAELGRAAHYDLTTITLGAQLSRHNV
                     EVSLDNEGAECWVDGLYLVGTGQHTDTHSLIDHRVPRCVSHQNYKGILDGRSRAVFNG
                     RVYVHPDAQQTDAFQSNKNLLLSNEARVDTKPQLEIFADDVKCAHGATVGQLAEEELF
                     YLVSRGLRPALARNLLTYGFAEEIVEKIKIGSIKRQLDEAILNRLHAEFEAQ"
     gene            1787..3055
                     /locus_tag="VF240_04185"
     CDS             1787..3055
                     /locus_tag="VF240_04185"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX7174467.1"
                     /translation="MSATDTAIINERVEGARGFDVERVRADFPVLSQTVNGKPLVYLD
                     NAASAQVPQIVVERGAKYLREEHSNIHRGVHYLSQRATTAFEGAREKVRRFINAREAR
                     ECIFTRGCTEGVNLVMYAYGRRVVGAGDEVVVSEMEHHSNIVPWQMLCEEKGARLRVI
                     PMNDDGELILDEFDALLGERTRLVCVNHISNALGTINPIKEIVARSHAQGVPVLVDGA
                     QGAPHLPIDVQDLDCDFYIISGHKMFAPTGSGVVYGKAALLEEMSPFHGGGSMIRSVT
                     FEKTTYAGIPEKFEAGTPAIASQIGLGAAIDYLNSLDRAAAHRHEQDLLTYATERLTA
                     IEGVRIIGAAREKASVLSFIIEDIHPHDIGTVLDYEGIAIRAGHHCAQPVMQHFKLPA
                     TARASFAFYNTREEVDRLAEAVEKVLEVLG"
     gene            3058..3543
                     /locus_tag="VF240_04190"
     CDS             3058..3543
                     /locus_tag="VF240_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX7174468.1"
                     /translation="MQDLKELYGEVITEHGKKPRNYRVMEAANRKAEGHNPLCGDHLL
                     LYLDLRDGRINDISFQGDGCRIFKASSSMMTHKLKGLGREEAKELFDEFHQMIVGELD
                     AENTPNKLGHLKAFSGVSNFPVRVKCASLPWRTLEAALEGQDTATSEDLGPPLSAAEP
                     G"
     gene            3661..4776
                     /gene="menC"
                     /locus_tag="VF240_04195"
     CDS             3661..4776
                     /gene="menC"
                     /locus_tag="VF240_04195"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX7174469.1"
                     /translation="MKIESIELREIRLPLVHFFETSFGRTTERRIMLARVRDAEGADG
                     WGECTAGEGPFYSDEWIDGAWEVTRQFLAPLTLGREVESAAAVFPLMRHVRGHRMAKA
                     SIETACWDAEAKRAGAPLWRHLGGERQEIACGVSIGIQDSVEQLLEKIATELAAGYQR
                     IKIKIKPGWDAGVVAKVRERFPDIRLMVDANSAYTLDDVALFRELDKYNLMMAEQPLA
                     HDDREDHAKLQARIETPVCLDESVHSPEDARKAIEMRACRIINVKLGRVGGHAEARRV
                     AEVCREGGIPVWCGGMLESGVGRAHNIARSTLAGFTLPGDVSASARYWEEDIIEPSVT
                     VTSRGTIIAPEGPGIGFRVNESRVDSLTVRKEILSAV"
     gene            4828..>5702
                     /locus_tag="VF240_04200"
     CDS             4828..>5702
                     /locus_tag="VF240_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7174470.1"
                     /translation="MSITPQTAGQIKTYFERRQTDVIALVRRLVELESPSGDADGSRA
                     VAALLVGVAREIPSVWTFEKISARESYGEHVRFKAFGGGERGRGILIVGHTDTVHPRG
                     SVGERPWREEGGKFYGPGVFDMKANCVLALEVLRCCAELDIKPKRPVTLLLTCDEESG
                     SANGGRELVEEEARAAECVLVLEPPAPGGRVKTARKGTGYYEVLVEGRAAHAGLEPEK
                     GVSAVHELARQILRLHELNEPARGTTVSVGVIGGGTHSNVVPAEARAEVDVRFSTLDE
                     AHRIDGLIRASEPFDG"
BASE COUNT         1086 a         1805 c         1856 g          955 t
ORIGIN      
        1 gggggggaga agaagcgcaa cgagattctg caaatggccg tgctcgaacc gaagctcgcc
       61 attctcgacg agaccgactc gggcctcgac atcgacgccc tccgcatcgt cgccgacggg
      121 gtcaatcaac tccgctcgcc gtcgaacgcc atcgtcctcg tcacacacta ccagcgtctt
      181 ctcaactaca tcgtgcccga ccacgtccac gtcctctttc aggggcgcat cgtccgctcc
      241 ggcgggcgcg agctggcgct ccagcttgaa gagaaggggt acgactggat tagagaagag
      301 gtcactactc agcaaacagc ggccggctaa tagagagcga acggcgagta gaaggaattc
      361 cttgtttatt gaacagcatt catagttacg gctatacggc tcgcggcccg gctttaagct
      421 aaccgctaac ggctgaaagc ttttttatgt cacacgcagt tatagagaaa agttcttacg
      481 tggatgcctt ccgcgagtcg cggcggctgg cgggcggaag cctgccgcgc tggttcgata
      541 acttgcgcgg cggctccatc gaacgctttg aggaagtcgg gtttccgacg accgacgagg
      601 aagattggaa gtacacgaac gtcgccccca tcgcgcgccg cgtctttcat ccggcgtcgg
      661 gacaggcgga agcggcgttt gactcctcgc tcatcgggcc atacgcctat caagaatcga
      721 aaggcagccg ggtcgtgttc gttgacggca tctaccgcgc ggacttgtcc tcgctggaag
      781 gattgccggc cggcgtcgag gtctcccaac tgaccgacgc gctggagggc gaacactccg
      841 aagtcgtccg caagcatctg gaagtggccg ccgctgctga ctcattcgcg gcgctcaaca
      901 cggccctgtt cgacgcgggg ctgctgctcc gcatcgggcg cggcgtggtc gtcgagagtc
      961 ccatccaact gctgttcctg gccgcttccg agcgtgaaga cacggcggcg ttcccgcgcg
     1021 tgctcatcgt ctctgagcct cagtcgtcgg cgacggtcat cgaaacttat gacgccgtcg
     1081 gcgatcaggt ttatttcact gactcggtcg tcgaggcccg cgtgggcgac ggcgcgcgtc
     1141 tcgtccacta ccgtgtgcag cgcgagggcg agcgggcctt ccacgtcgcc tccacgcgcg
     1201 ccgaactcgg ccgcgccgcc cactacgacc tgacgaccat cacgctcggg gcgcagctct
     1261 cccgtcacaa cgtcgaagtc tcgctcgaca atgaaggcgc cgagtgttgg gtggacggcc
     1321 tctatctcgt cggcactggg caacacacgg acacccactc gctcatcgac caccgcgtgc
     1381 cgcgttgcgt gagccatcag aactacaagg gcatcctcga cggccgctcg cgcgccgtct
     1441 tcaacggccg cgtctacgtc caccccgacg cgcagcagac cgacgccttc cagagcaaca
     1501 agaaccttct gctctcgaac gaggcccgcg tggacaccaa gccgcagctt gaaatcttcg
     1561 ccgacgacgt gaagtgcgcc catggcgcga ccgtcggcca gctcgcagag gaggagttgt
     1621 tctacctcgt gagccgcggc cttcggcccg cgctcgcgcg caacctcctg acctacggct
     1681 tcgccgagga gatcgtcgag aagatcaaga tcggctccat caagaggcag cttgacgagg
     1741 cgatactcaa ccgcctccac gccgaattcg aggcgcagtg aaaatgatgt cagcgacgga
     1801 cacagcaatc atcaacgaaa gagtagaggg tgcgcgcggc ttcgacgtgg agcgcgtgcg
     1861 cgccgatttc cccgtgctca gccagacggt caacggcaag ccgctcgtct atctcgacaa
     1921 cgccgcgtcg gcacaggtgc cgcagatcgt cgtcgagcgt ggcgcgaagt atctacgcga
     1981 ggagcactcg aacatccatc gcggcgtcca ctacctcagc cagcgcgcga cgacggcctt
     2041 cgagggagcg cgcgagaagg tgcgccgttt catcaacgct cgagaggctc gcgagtgcat
     2101 cttcacgcgc ggctgcaccg agggcgtcaa cctcgtgatg tacgcctacg gccgccgcgt
     2161 ggtcggcgcg ggcgacgagg tcgtcgtctc cgagatggag caccactcga acatcgtgcc
     2221 gtggcagatg ctctgcgagg agaagggcgc gcgcctgcgg gtcattccga tgaacgacga
     2281 cggggagttg attctcgacg agttcgacgc cctgctgggt gagcgcacgc gcctcgtctg
     2341 tgtcaaccac atctcgaacg cgctgggcac catcaacccc atcaaagaga tcgtcgcgcg
     2401 ctcgcacgcg cagggcgtgc ccgtcctcgt ggacggcgcg cagggcgccc ctcacctccc
     2461 catcgacgtg caagacctcg attgcgactt ctacatcatc tcgggccaca agatgttcgc
     2521 gccgacgggc agcggcgtcg tctacgggaa ggccgcgctc cttgaagaga tgagtccgtt
     2581 ccatgggggc ggaagcatga ttcggtcggt cacgttcgag aagacgacct acgccggcat
     2641 ccccgagaag tttgaggccg gcaccccggc catcgcctcg cagatcggac tcggagccgc
     2701 catcgactac ctcaactctc tcgaccgcgc cgccgcgcac cggcacgagc aagaccttct
     2761 cacctacgcc accgagcgtc tgacggccat cgagggcgtc cgcatcatcg gcgccgcgcg
     2821 cgagaaggcg agcgtgctgt ctttcatcat cgaagacatc cacccgcacg acatcggcac
     2881 cgtcctcgac tacgagggca tcgccatccg cgccggtcac cactgcgcgc agcccgtcat
     2941 gcagcacttc aagttgcccg ccacggcgcg cgcttccttc gcgttctaca acacgcgaga
     3001 ggaagttgac aggctggccg aggccgtcga gaaagtcctg gaggtgttgg gctaaacgtg
     3061 caggacttga aagaactcta tggcgaggtg attaccgagc acggtaagaa gccccgcaac
     3121 taccgcgtga tggaggcggc caaccgaaag gcggaaggcc acaaccctct gtgcggcgac
     3181 cacctgctgc tctatctcga cctccgcgac gggcgtatca acgacatcag ttttcagggc
     3241 gacggctgcc gcatcttcaa agcctcctcc agcatgatga cgcacaagct caaaggtctc
     3301 gggcgcgaag aggctaaaga actgttcgac gagtttcacc aaatgatcgt cggcgagctt
     3361 gacgccgaga acacgccgaa caagctcggg catctgaagg ctttctcggg cgtcagcaat
     3421 ttccccgtgc gcgtgaagtg cgcgagcctg ccgtggcgga cgctggaggc ggcactggag
     3481 ggtcaggaca cggccacgtc cgaagacctc gggcctccgt tgtcagcggc cgagccgggt
     3541 taagcttttg gattgacgga agtggaagta agtagggggc gaccgggtcg ccccctttcg
     3601 gttgtggggc gggtgtcgtt ataatgcgag cgcatttcct acttcgggcg gagtcaaata
     3661 gtgaaaatcg agtcgataga attgcgggag atacgcctgc cgctcgtcca cttcttcgag
     3721 acgagcttcg ggcgcacgac cgagcgccgc atcatgctcg cgcgcgtccg cgacgccgaa
     3781 ggggccgacg gctggggcga gtgtaccgcc ggtgaggggc cgttctactc ggacgagtgg
     3841 atcgacggcg cgtgggaggt gacgcgacag ttcctcgcgc cgttgacgtt ggggcgcgaa
     3901 gtcgagtcgg ccgccgcggt cttcccgctg atgcgacacg tccgcggcca ccgcatggcg
     3961 aaggcttcaa tcgagaccgc ctgctgggac gcggaggcga agcgagcggg cgcgccgctg
     4021 tggcgacacc tcggggggga acgacaggaa attgcctgcg gcgtctccat cggcattcag
     4081 gactcggtcg agcagctact cgaaaagatc gcgacggagc tggccgcggg ctaccagcgc
     4141 atcaagatca agatcaagcc aggctgggac gcgggcgtgg tggcgaaagt tcgcgaacgc
     4201 ttccccgaca tccgtctgat ggttgacgcc aactccgcct acacgctcga cgacgttgcg
     4261 ctctttcgcg aactcgacaa atacaacctg atgatggccg agcagccgct cgcgcacgac
     4321 gaccgagaag atcacgccaa acttcaagcg cggattgaga cgcccgtctg tctcgacgag
     4381 tcagtccatt cgccggagga cgcacgcaag gccatcgaga tgcgcgcgtg ccggatcatc
     4441 aacgtcaagc tcggccgcgt cggaggccac gccgaggcgc gtcgcgtggc ggaggtttgt
     4501 cgcgaaggtg gtatcccggt ctggtgcggc gggatgctgg agtcgggcgt gggacgcgcg
     4561 cacaacatcg cgaggtctac gcttgccggc ttcaccctgc cgggtgacgt gtcggccagc
     4621 gcgcgctact gggaagagga catcatcgag ccgagcgtca ccgtcacctc gcgcggcacg
     4681 atcatcgcgc ccgaggggcc gggcatcggc ttccgggtaa acgaatcgcg cgtcgattcc
     4741 ctgacggtgc gtaaagaaat tctaagcgca gtttgagacg ggcctatcgc cgcacacgtt
     4801 tgattggcga caaactccaa cgattaaatg tccatcaccc cgcaaacggc cggtcagata
     4861 aaaacatatt tcgagcgtcg tcagacggat gtgatcgcgc tcgtgcgccg gctcgtggag
     4921 cttgagtcgc cctcgggcga cgccgatggc agccgcgcag ttgcggcgct gctcgtcgga
     4981 gtcgcgcgcg aaatcccgtc ggtctggacg tttgaaaaaa tcagcgcgcg ggagagctac
     5041 ggcgaacacg tgcggttcaa ggcgttcgga gggggcgagc gcggccgtgg aattttgatt
     5101 gtagggcaca cggacacggt gcatccgcgc ggctccgttg gcgagcggcc gtggcgagaa
     5161 gaaggcggga agttttacgg ccccggcgtc ttcgacatga aggccaactg cgtgctcgca
     5221 ctcgaagtcc tgcgttgctg tgcggagttg gacatcaaac cgaaaaggcc ggtcactctg
     5281 ctcctgacgt gtgacgagga atcgggcagc gccaacggtg gtcgcgaact cgtcgaggag
     5341 gaagcgcgcg cggccgagtg cgtgctggtg cttgagccgc cagcgccggg cgggcgcgtg
     5401 aagacggcgc ggaaggggac gggctattac gaagtcttgg tcgaggggcg ggccgcgcac
     5461 gccgggctgg agccggagaa gggcgtaagc gcggtacacg aactcgcccg tcagattttg
     5521 cgcctgcatg agttgaacga accggcgcgt gggacgaccg tcagcgtcgg cgtcataggc
     5581 ggcggcacgc actcgaacgt cgtccccgcc gaggcgcgcg ccgaggtgga cgtgcgtttc
     5641 agcacgctcg acgaggcgca caggattgac ggtcttatcc gcgcctccga gccgttcgac
     5701 gg
//
LOCUS       DASVES010000114         6311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_476865, whole genome shotgun sequence.
ACCESSION   DASVES010000114 DASVES010000000
VERSION     DASVES010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476865"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..324)
                     /locus_tag="VF240_04205"
     CDS             complement(91..324)
                     /locus_tag="VF240_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174471.1"
                     /translation="MNESRERNVPDGSDEKAGHSGDEAATSKETLSDVEGSEKVSTPR
                     ETGSPTETSSPGPSPDGSAGEGRADGSETGGPM"
     gene            complement(336..950)
                     /locus_tag="VF240_04210"
     CDS             complement(336..950)
                     /locus_tag="VF240_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX7174472.1"
                     /translation="MKVVKESSDELNPTVVVTDAFIERAPRRRTWRESAAVAIATCGV
                     GFLPVAPGTWGSAVGVGLYLLARVVSTQIFAEATARGVSLPPLEAFRTTFVLLLIIGV
                     TLAGTWAATRVESVLKVKDPGVVVIDEVAGQLITFLFVPWDSGAWVIVAGFLAFRVFD
                     IWKPYPIRRLESLESGLGVMADDVLAGAYAATLLSLLTSIRLMI"
     gene            complement(1034..2395)
                     /gene="rimO"
                     /locus_tag="VF240_04215"
     CDS             complement(1034..2395)
                     /gene="rimO"
                     /locus_tag="VF240_04215"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX7174473.1"
                     /translation="MKKVGFISLGCPKNLVDSEVMMGRLREQGYEITPDASEAETVVV
                     NTCGFIDAAKKESIDAILEAARLKSDGNCRRLVVAGCLVERYRDELRAEMPEVDAFIG
                     TNQIDDITNVCDEKTNTRQLPVLQLGNQTATYLYDESTPRVLATPSHYAYLKIAEGCD
                     RPCAFCFIPQMRGHFRSRRFGSVVAEARSLAAQGVKELILVAQDSSRYGEDLGEPDAL
                     AHLLRELSHLDGVEWVRVMYTYPTHISDGFLDVLASEPKAVKYLDMPLQHASQNVLKL
                     MRRGGNRESLERLIKRVRDRVPGIAVRTTFITGFPGETEGDFEELLAFVRNVGFDRVG
                     VFTYSDEEGTPAFDLPDKVDARTAARRRTKLMKEQARISKKKNKARVGQTVRVLFEGP
                     SAETDLLWQGRTEAQAPDIDGCVLINDAPEGCAPSPGDIVNVLITEAQQYDLVGEIVS
                     SEQ"
     gene            complement(2456..4780)
                     /locus_tag="VF240_04220"
     CDS             complement(2456..4780)
                     /locus_tag="VF240_04220"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX7174474.1"
                     /translation="MTFDTWFRASSYATVACGAAALVVVGGVGWATTLVFAALLALAW
                     SLEGKRWQLSERAGLLVVLVSLPLFYLDWQYQEAAAGGVEAGAGLAALKHLTLFLSAV
                     KLLQVKSDRDWLFLYLVAFFEVLLAAGLSVSPLFLVALAAYLFCALLSVVCFELRKAR
                     RAAAPDARVRVTFDSARRRRDATADGVAQALRRLPAVALCLLALVFALALPIFFVTPR
                     LGTRASSLGNVGPTAQVGFSDQVTLGDFGRIQKSDRLVMRVSVGGAQAVGSQPLRWRG
                     VSLDHFDGQTWRRSSGESQEVTGGESDFFPLGTTEHLGRLTTQTFFVEPLDTAVLFAA
                     PRVVAVEGALDFVRSYKDGGALTTRPHPRERISYRAYSDTVEQAAAALRADDRRYPRA
                     RTPNLQAPIENYLRYPAELDAGVVSLAWHVANLAGARSRYDMARAIESHLGRNEYGGA
                     YSYSLETRGGGDDPLADFLFNVRAGHCEYFSTAMAVMLRTQGVAARVVNGFQAGEYNA
                     AAGAFFVRQYDAHSWVEVYFPETDSWVAFDPTPAAGRPGAGASASFGARLMKYAEALE
                     LFWIRYVIAYDSREQQTIASSIRDSFSSLRRAVAATAAGLGTRLSSIWGGRETRDGVG
                     TAGAGFPLVRTLVAVAAVGLLLLVFVRRWLKARGERERVGAQGGGASAVDFYERMATA
                     LDARGLRRPTHQTPLEFASATGVPEALTITKAYHRVRFGAQSLSPAEAAEVERCLRQL
                     EGEEPSIVNRNRQSSIDSTDDASDISNHDAYSRR"
     gene            complement(4914..5783)
                     /locus_tag="VF240_04225"
     CDS             complement(4914..5783)
                     /locus_tag="VF240_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX7174475.1"
                     /translation="MTLTPARILSLLLAPTLLLLCVGCQQQAARDTGASNSGARSGAT
                     SNSMSNDGTTRQLPVYGYEVVNSWPHDREAFTQGLVFHEGALIESTGQYGESSLRRVE
                     LQTGRVLQKTDVPPQFFAEGATLLRGKLYQLTWTTERGFVYDPATLKKTGEFRYSGEG
                     WGLTHDGESLILSDGTNQIRFLNPDTFAVSRTINVFDRGRPVREINELEYVKGEIYAN
                     VWKTDRVARIDPQTGALKGWIDLKGIISTAERSGDTDAVLNGIAYDERGDRLFVTGKL
                     WPKLFEIRLKEGR"
     gene            complement(5905..6021)
                     /gene="rrf"
                     /locus_tag="VF240_04230"
     rRNA            complement(5905..6021)
                     /gene="rrf"
                     /locus_tag="VF240_04230"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(6115..>6311)
                     /locus_tag="VF240_04235"
     rRNA            complement(6115..>6311)
                     /locus_tag="VF240_04235"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1125 a         2051 c         2026 g         1109 t
ORIGIN      
        1 tcaggagtca ggagtcagga gttagaatga ataccgcgtt cgccctcttc attctggctc
       61 ctgactcctg actcctgaat actttctccc ctacatcggg ccgccggtct cgctgccgtc
      121 ggcgcgtcct tctccggcgg agccgtcggg cgagggaccg ggcgaggagg tttcggtcgg
      181 cgagccggtc tcgcgaggcg tcgaaacctt ctcgctgccc tcaacgtcgc tcagggtctc
      241 cttgctggtc gccgcttcgt cgccgctgtg gcccgccttt tcatcggagc cgtcgggcac
      301 attgcgttct ctggattcgt tcataatccc tccctttaaa tcatcagtct gattgaagtc
      361 agaagcgaca acagcgtcgc cgcgtaggct ccggccagca catcgtcggc catgacgccg
      421 aggcccgact ccagcgattc gagccggcgg atcgggtacg gcttccagat gtcgaagacg
      481 cggaacgcga ggaagcccgc gacgatcacc cacgcgcccg agtcccacgg cacgaagagg
      541 aacgtgatca actgcccggc cacctcgtcg ataaccacca cgccggggtc tttcaccttc
      601 agcaccgact cgacgcgcgt cgccgcccac gtccccgcca gcgtcacgcc gatgatgagc
      661 agcagcacga acgtcgtgcg gaaagcttcg agcggcggca gactcacgcc gcgcgccgtc
      721 gcctccgcga agatttgcgt gctcacgacg cgcgcgagca aatacagtcc aacgccgacg
      781 gccgagcccc acgtccccgg cgcgaccggc agaaagccga cgccgcacgt cgcgatggcg
      841 acggccgccg actccctcca cgtgcgcctg cgcggcgcgc gctcgatgaa ggcgtcggtg
      901 acgaccaccg tcgggttcag ctcgtcactc gattctttga caaccttcat aaagtgagca
      961 gtgagcggta agcagtgagc agtatggttg tccgcgatga gcgttgagca ctaggctttt
     1021 aactgcttac tgcttactgc tcactgctca ctatttcgcc gacgaggtcg tactgctgcg
     1081 cctcggtgat gagcacgttg acgatgtcgc cgggcgaagg ggcgcagccc tcgggcgcgt
     1141 cgttgatgag gacgcagccg tcgatgtcgg gagcctgcgc ctccgtgcgg ccctgccaga
     1201 gcaggtcggt ctcagccgat ggcccctcga acaacacgcg cacggtctga cccacgcgcg
     1261 ccttattctt cttcttcgag atgcgcgcct gctccttcat caattttgtg cggcgacggg
     1321 cggcggttct ggcatcgacc ttgtcgggca gatcgaaggc cggagtccct tcctcgtccg
     1381 agtaggtgaa gacgccgacg cggtcgaacc cgacgttgcg gacgaaggcc agcagctcct
     1441 cgaagtcacc ttcggtctcg ccggggaagc ccgtgatgaa cgtcgtgcgg acggcgatgc
     1501 cggggacgcg gtcgcggacg cgcttgatca gacgttcgag actctcgcga ttgccgccgc
     1561 gccgcatcag cttcagcacg ttctgcgagg cgtgctgcaa aggcatgtcg agatatttga
     1621 cggccttcgg ctcggaggcc agcacgtcta agaagccgtc gctgatatgc gtcgggtagg
     1681 tgtacatgac gcgcacccac tcgacgccgt caaggtgcga aagctcacgg agcaagtgcg
     1741 cgagcgcgtc cggctcgccg aggtcttcgc cgtagcgcga agagtcctgc gcgacgagga
     1801 tgagttcctt gactccctgc gcggcgagcg agcgcgcctc ggcgacgacg gagccgaagc
     1861 gacggctgcg gaagtggccg cgcatctgcg ggatgaagca gaaggcgcag gggcggtcgc
     1921 agccctcggc gatcttcaag taggcgtagt gagacggcgt ggcgagcacg cgcggcgtcg
     1981 attcgtcgta gaggtaagtg gcggtctgat taccgagctg gagcacgggg agttgccgcg
     2041 tgttggtctt ctcgtcgcag acgttcgtga tgtcgtcgat ttgattcgtg ccgatgaagg
     2101 cgtccacctc gggcatctcg gcgcgcagct cgtcgcggta gcgttcgacg aggcacccgg
     2161 cgacgacgag gcggcgacag ttgccgtccg acttcaaacg cgcggcttcg aggatggcgt
     2221 cgatagactc cttcttcgcc gcgtcgatga agccgcaggt gttgacgacg accgtctcgg
     2281 cctcgctcgc gtccggcgta atctcatacc cctgctcgcg cagccgcccc atcatcactt
     2341 cgctgtcaac cagattcttc ggacagccca gactgataaa tcctactttt ttcataaagc
     2401 gttaatcgtc aatcgtcaat cgtgaatggt aaatcgtaaa tcgtgtttga gtggcctacc
     2461 gccttgaata tgcgtcgtgg tttgaaatat ctgaagcatc gtcagtcgaa tcgattgacg
     2521 attgacgatt tcgattgacg attgacggct cttccccctc caactgccgc aggcagcgtt
     2581 ccacctccgc ggcctccgcc ggcgataggc tctgcgcgcc gaagcgcacg cggtggtacg
     2641 ccttcgtaat cgtcagggct tcgggcacgc cggtcgcgga ggcgaactcc agtggcgtct
     2701 gatgcgtcgg acggcgcaag ccccgcgcgt cgagcgccgt cgccatccgc tcgtaaaaat
     2761 ccacggccga cgcgccgccg ccttgcgccc ccacgcgctc acgctcgccc ctcgccttca
     2821 accagcgtcg gacaaacacg agcagcagga ggcccacggc ggccacggcg acgagtgtcc
     2881 gcaccagcgg gaagcccgcg ccggccgtgc cgacgccgtc ccgcgtctca cgtccacccc
     2941 atatggagga cagtctggtc cccagtccgg cggccgtcgc cgcgacggcc cggcgtaacg
     3001 agctgaagct gtctcttata gaactcgcga ttgtttgctg ctcgcgacta tcataagcga
     3061 taacgtaccg aatccagaaa agctccagcg cctcggcgta tttcatcagc cgcgcgccga
     3121 aggacgcgga agcgcccgcg cccggtcgcc cggcggccgg cgtcgggtcg aaggccaccc
     3181 acgagtcggt ctccgggaag tagacctcga cccacgagtg cgcgtcgtac tgccggacga
     3241 agaaagcgcc ggccgccgcg ttgtactcgc cggcctggaa accgttgacg acgcgcgccg
     3301 ccacgccctg cgtccgcagc atgacggcca tcgcggtgga gaagtattcg cagtgccccg
     3361 cgcgcacgtt gaagagaaaa tcggcgagcg ggtcgtcgcc gccgccgcgc gtttcaaggc
     3421 tgtaggaata agcgccccca tactcgttgc ggccgagatg tgactcgatg gcgcgcgcca
     3481 tgtcgtagcg gctgcgtgcg ccggccagat tcgcgacgtg ccacgcgaga ctgacgacgc
     3541 cggcgtcgag ttccgccggg taacgcagat agttctcaat cggcgcctgt aggttcggcg
     3601 tccgcgcacg cggatagcgc cggtcgtcgg cgcgtaaggc ggcggccgcc tgctcgaccg
     3661 tgtccgaata ggcgcgatag ctgatgcgct cgcgcgggtg cgggcgggtg gtgagcgcgc
     3721 cgccgtcttt gtagctgcgg acgaagtcga gcgcgccctc gacggcgacg acgcgcggcg
     3781 cggcgaagag gacggccgtg tctaacggct cgacgaagaa ggtctgcgtc gtcagccggc
     3841 cgagatgttc ggtcgtcccc aacgggaaga agtcgctctc gccgcccgtg acttcctgac
     3901 tctcaccgga ggagcgtcgc cacgtctgcc cgtcgaagtg gtcgagtgaa acgccgcgcc
     3961 agcgtagcgg ctggctgcct accgcctgcg cgcccccgac gctgacgcgc atcaccagcc
     4021 ggtcgctctt ctggatgcgg ccgaagtcgc cgagcgtaac ctgatcggag aagcccacct
     4081 gcgcggtcgg gccgacattg ccgagcgagg aggcgcgcgt ccccagccgc ggcgtgacaa
     4141 agaagatcgg cagggcgagc gcgaagacga gcgccagcag gcacaacgct acggccggaa
     4201 gccgccgcag ggcttgagcg acgccgtcgg ccgtcgcgtc gcgtcgccgt cgggcggagt
     4261 cgaacgtcac gcgcacgcgc gcgtccgggg ccgcggcgcg gcgcgccttc cgcagctcga
     4321 agcagaccac gctcagcaac gcgcagaaga ggtaggcggc gagcgccacg aggaagagcg
     4381 ggctgacgct caggcccgcg gccaacagca cctcgaagaa cgcgacgagg tagaggaaca
     4441 gccagtcgcg gtcggacttg acctgaagga gtttgacggc tgaaaggaag agcgtgagat
     4501 gtttgagtgc ggcgaggcca gcgcccgctt caacgccgcc ggccgccgcc tcctgatatt
     4561 gccagtcgag ataaaagagc gggagcgaga ccagcacgac caacaggcca gcgcgctctg
     4621 agagttgcca cctcttcccc tccagtgacc acgccagcgc caacaacgcg gcaaacacca
     4681 gagtcgtcgc ccagcccacg ccgcccacga ccacgagcgc ggccgcgccg cacgctacgg
     4741 tcgcgtagga tgacgcgcgg aaccaggtgt cgaaagtcat gcttctgaga tgcggtcagg
     4801 ctgtccgtcg tcagtggtca gatgtttgtt tgcggcgact cgccgccgac accggaaaag
     4861 ctcaaggcgt gggacgaaaa caactgacaa ctgaccgtgg acaactgaca gttttatcgc
     4921 ccttccttga gccgaatctc gaagagcttg ggccagagct tgccggtgac gaagagacgg
     4981 tcgccgcgtt cgtcgtaggc gatgccgttt aagacggcgt cggtgtcgcc gctgcgctcg
     5041 gccgtggaga tgatcccctt gaggtcgatc cagcccttga gcgcacccgt ctgcgggtcg
     5101 atgcgcgcga cgcggtcggt cttccagacg ttggcgtaga tttcgccctt cacgtattcg
     5161 agttcgttga tctcgcgcac ggggcggccg cggtcgaaga cgttgatggt tcggctgacg
     5221 gcgaacgtgt cggggttgag gaagcgtatc tggttggtgc cgtcgctcag gatgaggctc
     5281 tcgccgtcgt gcgtgaggcc ccacccttcg ccgctgtagc ggaactcgcc ggtcttcttg
     5341 agcgtcgccg ggtcgtagac gaagcccctc tccgtcgtcc acgtcaactg gtagagcttg
     5401 ccgcgcagca gcgtcgcgcc ctcggcgaaa aactgcggcg gcacgtccgt cttctggagc
     5461 acgcggcccg tttgcaactc gacccgtcgc agcgacgact cgccgtactg tcccgtgctc
     5521 tcgatgagcg cgccctcgtg gaagaccaga ccctgtgtga acgcctcgcg gtcgtgcggc
     5581 caggagttga ccacctcgta gccgtagacc ggcaactgtc gtgtcgtgcc gtcgttgctc
     5641 atcgagttag acgtggcgcc cgagcgggcg ccgctgttgg aggctcccgt atcacgcgcc
     5701 gcctgctgct ggcaacccac gcacaaaagc aggagcgtcg gcgcgagtag gagggacagg
     5761 attctggccg gagtgagcgt catctgtttg gccccgcatg gaggtttctt gtgatagcag
     5821 accagtcccg attatcgccg caagacgcac aaagacaaaa gcccgcgcgg gatgtccggc
     5881 gcgggctttc tcgtcaaaaa ttaaatccgg cggcgaccta ctctcccacg cagtcccccg
     5941 cgcagtacca tcggctctga gaggcttaac ttccgtgttc gggatgggaa cgggtgtgac
     6001 cctctcgata taaccaccgg aaaacttgaa agaacatgct cgctaagaga gcttggacaa
     6061 tcgaatagtg aacttagctg tgcaaaccgc agctgttgct tgagagttaa ttatacggtc
     6121 aagccttggg actgattagt actggtcagc tacacgcatt actgcgcttc cacacccagc
     6181 ctatcaacgt ggtggtctac cacgagtctc actggcaggt ctcatcttgg gtctggcttc
     6241 acgcttagat gcattcagcg tttatccatg ctccacgtag ctaccgagcg ctacccctgg
     6301 cgggataact c
//
LOCUS       DASVES010000115         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1397226, whole genome shotgun sequence.
ACCESSION   DASVES010000115 DASVES010000000
VERSION     DASVES010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397226"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VF240_04240"
     CDS             <1..194
                     /locus_tag="VF240_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174476.1"
                     /translation="MHDELEREGVSYDQTDGMKISWPDGWVHVRVSNTESMIRLIAEA
                     EDVARASELLDWARDRVGR"
     gene            232..1197
                     /locus_tag="VF240_04245"
     CDS             232..1197
                     /locus_tag="VF240_04245"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7174477.1"
                     /translation="MKKLLLAPLLMLAASASGGLPPESGVVAVKVAEAPGYTEGVVID
                     ADGVVYISDVYNGTIYQIGADGRATSWSVTGAPNGHKILADGTHLVCDGSQHAVLRLD
                     SAGKVMGKASSECDGKPLRAPNDLTLDQRGGFYFTDPGGSGKQNPIGTVHYVDAQGKT
                     HLAAEGLAFPNGIALRPDGKTLLVGESHYNRILSYEVISPGRLGKMRVFANLPVKEGE
                     QIANEPDGICLDAAGNLYVAHYGMRQVQVLSPRGKLLRRYGAGNLTTSNVAFGGPRMD
                     RLYVTGAIGEEKTSRGGLFRLDLKGVKGLKILPRRQTSPARGAKR"
     gene            1202..>3477
                     /locus_tag="VF240_04250"
     CDS             1202..>3477
                     /locus_tag="VF240_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7174478.1"
                     /translation="MRKLCSAIAALSFFVSGFVPADGQTPASPPSAEVERRVESLLRQ
                     MTIEEKIDLLGGVDGFFVRGVERLKVPRLKMADGPNGVRHYGPATAMPSGIALAATWN
                     EQLARRVGAEIGRDARAKGVHFLLGPGVNIYRGPLNGRNFEYYGEDPFLAARTTVGYV
                     RGVQSQNVAATVKHYMGNNSEFDRHKTDSIIDERTMREIYLPAFEAAVREAKVGAIMD
                     SYNLINGVHASQHAYLNNEVAKREWGFDGIIMSDWGGTYDAVGAANGGLDLEMPSGAF
                     LNREKLLPAIEQGKVSVATIDEKVRRILRTAARFGWLDRDQTDLSVPRYNQQGRRVAL
                     EAAREGMVLLKNEGNVLPLDKAKIKSVAVIGPASYPAVPMGGGSGRVEPFAAVSFLEG
                     LSNALGTSANVYHHRGLPTLGEWAEATKFSTDATGAQPGLLAEYFDNPELKGEPVVKR
                     TESNVNFRKGGRASFPEKTAAERWTGYFVPPQAGTYDIFVEATGEDGGWYRLYVDDKL
                     VIDQWATARALLNYLSLPFDARPHKVVLEHRGRSNWLGTRLGLGIVRQGGAVDPAATA
                     LAARADVVIVAAGFDPESESEAADRTFRLPPGQEELIRELTAANRNTVVVLTSGGGVD
                     MGAWVERVPALVEAWYPGQEGGTALAEILLGDVNPSGRLPVTFERRWEDNPAHASYYP
                     EGDTKRVVYKEGVFVGYRGYEKTGARPLFPFGHGLSYTTFRYGNLSVKPVVGRGAEDS
                     GPRYEVSFDVRNTGAREGA"
BASE COUNT          625 a         1132 c         1169 g          551 t
ORIGIN      
        1 ggatgcacga cgagttggag cgcgagggcg tgagttacga tcagaccgac ggcatgaaaa
       61 tctcgtggcc cgatgggtgg gtacacgtgc gcgtctcgaa cacggaatcg atgatccgtc
      121 tcatcgcgga ggccgaagac gtggcgcgcg cgagcgaact gctcgactgg gcgcgcgacc
      181 gcgtgggccg gtgagcgata acttcaactt cttactttac ggtaaaactc catgaagaaa
      241 cttcttctcg cccccctcct gatgctcgcc gcatccgcgt cgggcggcct cccgcccgag
      301 agcggcgtcg tggccgtcaa agttgcggag gcgcccggct acaccgaggg cgtcgtcatc
      361 gacgcggacg gcgtcgtcta catctccgac gtttacaacg gaaccatcta ccagatcggg
      421 gccgacggcc gggcgacctc gtggtcggtg acgggcgcac ccaacggcca caagattctc
      481 gccgacggca cacacctcgt ctgcgatggc agccagcacg cagtgctgcg tctcgactcc
      541 gcgggcaagg tcatgggcaa ggcgtcctcc gagtgcgacg gcaaacccct gcgcgcgccc
      601 aacgacctga cgctcgacca gcgtggcggc ttctatttca cagaccccgg cggttccggc
      661 aaacagaatc caattggaac agttcactat gttgacgcgc agggcaagac tcatctcgcg
      721 gcggaggggc tggcgtttcc gaacggcatc gccctgcgcc ccgacggcaa gacgttgctc
      781 gtcggcgaga gccactacaa ccgcatcctg agctacgaag ttatttctcc cggccggctc
      841 gggaagatgc gcgtctttgc gaacctcccg gtgaaggagg gcgagcagat agcgaacgag
      901 cctgacggca tctgtctcga cgcggccggc aacctctacg tcgcacacta cggcatgcgt
      961 caggtgcagg tcttaagccc ccgaggcaaa ctcctgcgcc gttacggggc gggcaatctc
     1021 accacgagca acgtggcctt cggcggcccg cggatggatc ggctctacgt cacgggggcc
     1081 atcggcgaag agaagaccag ccgcggcggc ctcttccgcc tcgacctcaa gggcgtgaag
     1141 ggattgaaga ttcttccacg cagacagacc agccccgcgc gcggcgcgaa gaggtgaatc
     1201 gatgagaaaa ctctgttcgg caatcgcggc tctgtccttc tttgtctctg gcttcgtccc
     1261 tgcggacgga caaactcccg cgtcgccgcc gtcggccgag gtcgagcgcc gcgtcgagtc
     1321 tctgctccgg cagatgacga ttgaggagaa gatcgacttg ctcggcggcg tggacggctt
     1381 cttcgtccgc ggcgtcgaac gcctcaaggt tccgcgtctg aagatggccg atgggccgaa
     1441 cggcgtccgc cactacggcc cggccacagc catgccgagc ggtatcgcgc tcgccgccac
     1501 gtggaacgaa caactcgcac ggcgcgtcgg cgccgagatc gggcgcgacg cgcgcgccaa
     1561 gggcgtccac ttcctgctgg ggccgggggt gaacatctat cgcggcccgc tcaacggccg
     1621 caacttcgag tactacggcg aagacccctt cctcgccgcg cgcacgaccg tcggctacgt
     1681 ccgcggcgtg cagtcgcaga atgtcgccgc caccgtcaag cactacatgg ggaacaactc
     1741 cgagttcgac cggcacaaga ccgactccat tatcgacgag cgcacgatgc gcgaaatcta
     1801 cctgccggcg ttcgaggcgg cggtgcgcga ggcaaaggtc ggggccatca tggactcata
     1861 caacctcatc aacggggtgc atgcgtcgca gcacgcctat ctcaacaacg aagtggcgaa
     1921 acgcgagtgg ggcttcgacg gcatcatcat gtccgactgg ggcggcacct acgacgccgt
     1981 cggcgccgcc aacggcggcc tcgatctgga gatgccctcc ggcgctttcc tcaaccgcga
     2041 gaaacttctg ccggccatcg agcagggcaa agtctccgtc gccaccatcg acgagaaggt
     2101 gcgccgcatc ttgcgcacgg ccgcgcgctt cggctggctc gaccgcgacc agaccgacct
     2161 ctccgtcccg cgctacaacc agcagggccg gcgcgtggcg ctcgaagcgg cgcgcgaggg
     2221 gatggtgctc ttgaagaacg agggcaatgt gcttccgctc gacaaggcga agattaaatc
     2281 cgtcgccgtc atcgggccgg cgagttaccc ggcggtgccg atgggcggag gcagcgggcg
     2341 cgtggagccg ttcgccgccg tcagctttct ggagggtttg agcaacgcgc tcggcacgtc
     2401 cgcgaacgtc tatcaccacc gcggcctgcc cacactcggc gagtgggccg aggcgacgaa
     2461 gttctccacc gacgcgacgg gcgctcagcc cggtctgctc gcggaatatt ttgataaccc
     2521 tgagctgaag ggcgagccgg tcgtcaaacg caccgagtcg aacgtcaact tcaggaaggg
     2581 cgggcgcgcg tcgttcccgg agaagaccgc cgccgagcgt tggacgggct acttcgtccc
     2641 gccgcaagcg gggacgtatg acatcttcgt cgaggcgacg ggcgaagacg gcggctggta
     2701 tcgcctgtac gtggatgaca agctcgtcat cgaccagtgg gcgacggcgc gcgcgctcct
     2761 caactacctg agccttccat tcgacgcgcg tccgcacaag gtcgtgctcg aacaccgcgg
     2821 gcgctcgaac tggctgggca cgcggttggg gttgggaatc gtgcgtcagg ggggagcagt
     2881 tgacccggcg gccacagcgc ttgccgcgag agcggacgtg gtgattgtcg ccgccgggtt
     2941 cgaccccgag tcggagagcg aggcggcgga tcgcacgttt cggcttccgc cgggacagga
     3001 ggaattgatt cgagagttga ccgccgcgaa ccggaacaca gtcgtcgttc tcacctcggg
     3061 cggcggggtg gacatgggcg cgtgggttga gcgcgtgccg gcgctcgtcg aggcgtggta
     3121 tccggggcag gagggcggca cggcgctggc cgaaatcctc ctcggcgacg tgaacccttc
     3181 ggggcgtctg cccgttacct tcgagcgaag gtgggaagac aacccggccc acgcgagcta
     3241 ctaccccgaa ggggacacga agcgtgtcgt ctacaaggag ggcgtcttcg tcggctaccg
     3301 cggctacgag aagacgggcg cgcgtccgct cttcccattc gggcacgggc tgtcgtacac
     3361 cacgttccgc tacggcaatc tttccgtgaa gccggtcgtg ggccggggcg cggaagactc
     3421 ggggccgcgc tacgaggtct cgttcgacgt gaggaacacg ggcgcgcgtg aaggcgc
//
LOCUS       DASVES010000116         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1415786, whole genome shotgun sequence.
ACCESSION   DASVES010000116 DASVES010000000
VERSION     DASVES010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415786"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1031
                     /locus_tag="VF240_04255"
     CDS             135..1031
                     /locus_tag="VF240_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7174479.1"
                     /translation="MSDALFGGNSAITNGAGEHTCRRVLLGEVEDVRARLIRALERLD
                     YRLLNEQQPLLARRAARKEIISADMLDVARTLAINLKPSGTAATLAIFDITVVHGQPW
                     GMGKGDRHTLELEIDALVALAAAPDEQSACALCGTENSGGARFCRSCGAPNNFREPAE
                     VELMRLEAGARAAHQEHVFGFLIMLVVAVLALPLIIFSSNKVAPIGWVVFAAGMLLGC
                     LMTLYGVRRLHRTLNPKERKSGQTTPAQPEAIPAPRAAALPPRSARTSVTEGTTELLH
                     TEARERVEVRPRARGGNTDPIE"
     gene            1087..2322
                     /locus_tag="VF240_04260"
     CDS             1087..2322
                     /locus_tag="VF240_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX7174480.1"
                     /translation="MLTAEIIAIGSELLTPERTDTNSLWLTEKLNSVGIEVKLKTIVG
                     DDDARLEESIRDALRRSGVIVTTGGLGPTEDDITRKIAARALGRRIMLDERVLEQIRE
                     KFARWGRKMPEINSRQAMVIEAAEVLDNPQGTAPGMLLEHEGRTVVLLPGPPREMRPM
                     FETHVQPRLAAKAGDVRVVRRVLRVTGMGESAVDERIAPVYTQYKNPQTTILFNKSEI
                     EIHLTAQGKTEQEAELLIDGLSGQLEERLGDSIFAFRGERIEEVVGLRLAVTGFTLAL
                     AESCTGGLIAERLTEVPGSSVYFKEAIVAYSNEAKVRLLGVPSDLIAEHGAVSAPVAE
                     AMAEGARLRADTDFGLSVTGIAGPGGGSEEKPVGLVYIALSDDAHTEHRRLLLPGDRQ
                     LIRWRAAQAALDLLRRRLI"
BASE COUNT          428 a          863 c          815 g          356 t
ORIGIN      
        1 aatgaggggc ccgcggcgcg cacgcgagac ccgcgaggct accgctcgcg ctactgttgc
       61 ccatggcgga aacgcgccgg ccctcccgtt cgtaaataca caggagttgt gaaacgtcag
      121 caggaggtgc ggagttgtca gacgctttgt ttggcgggaa ctcggcaatc accaacggcg
      181 cgggcgagca cacgtgccgg cgcgtgctcc tcggcgaggt cgaggacgtg cgggcgaggc
      241 tcatccgcgc gctcgaaagg ctcgactacc gccttctgaa cgagcagcag ccgctgctcg
      301 cgcgccgcgc cgcccgcaag gaaatcatca gcgccgacat gctcgacgtg gcgcgcaccc
      361 ttgccatcaa cctcaagcca agcgggacgg cggccacgct cgccatcttc gacatcacgg
      421 tcgttcacgg ccaaccctgg ggcatgggca agggcgaccg ccacacgctc gaactggaga
      481 ttgacgccct cgtcgccctt gccgccgcgc ccgacgagca gagcgcctgt gccctctgcg
      541 ggacggaaaa ctccggcggg gcacgcttct gccgctcgtg cggcgcgccc aacaacttcc
      601 gcgagccggc cgaggttgag ttgatgcgtc ttgaagcggg cgcgcgcgcc gcacatcagg
      661 agcacgtctt cggctttctc atcatgctcg tcgtcgccgt gctggcgctg ccgctcatca
      721 tcttcagcag caataaggtc gcgcctatcg gctgggtcgt cttcgccgcg ggcatgctcc
      781 tcggctgcct gatgacgctc tacggcgtgc gccgcctgca ccgcacgctt aacccgaagg
      841 agcgcaagag cggacaaacg acgcccgcgc agcccgaagc catccccgcg ccgcgcgccg
      901 ccgcgctccc gccgcgctca gcccgcacgt ccgtgacgga ggggacgacc gagttgctgc
      961 acaccgaggc gcgcgagcgt gtcgaggtgc ggccacgggc gaggggcggc aatacagacc
     1021 cgattgagtg aacgaaagtc gttcctgacg gcgacgcgaa acgcgcacct gagttaaact
     1081 cccgacatgc tgaccgccga gatcatcgcc atcggctcgg agcttttgac gcccgagcgc
     1141 accgacacca acagcctctg gctcaccgaa aaactcaaca gcgtcggcat cgaggtcaag
     1201 ctcaagacca tcgtcggcga cgacgacgcg cgtctcgaag agtcgatccg tgacgcgctg
     1261 cggcgctccg gcgtcatcgt cacgaccggc ggtctggggc cgaccgagga cgacatcacg
     1321 cgcaagatcg ccgcccgcgc cctcggtcgc cgcatcatgc tcgacgagcg cgtgctcgaa
     1381 cagattcgcg agaagttcgc gcgctgggga cggaagatgc ccgagatcaa ttcgcgtcag
     1441 gcgatggtca tcgaggcggc ggaagttttg gacaacccgc aggggacggc gccggggatg
     1501 ctgctcgaac atgagggacg cacggtcgtg ctgctgccgg ggccgccgcg cgagatgcgg
     1561 ccgatgttcg agacgcacgt ccagccgagg ctcgcggcca aagccggcga cgtgcgcgtc
     1621 gtccggcgcg tgctccgcgt gacggggatg ggcgagtcgg ccgtggacga gcgcatcgcg
     1681 cccgtctaca cgcagtacaa gaacccgcag acgaccatcc tcttcaacaa gagcgagatc
     1741 gaaatccacc tcaccgcgca gggcaagacc gaacaggagg ccgagctttt gatcgacgga
     1801 ctgtcggggc aactcgaaga gcggctcggc gactcgatct tcgccttccg aggcgagcgc
     1861 atcgaggagg tggtcggcct gcgcctcgcc gtcaccggct tcacgctcgc gctcgccgag
     1921 agctgcaccg gcggactcat cgccgagcgt ctgacggaag ttcccggctc ctccgtctac
     1981 ttcaaagagg ccatcgtcgc ctactcgaac gaggcgaagg tgcgcctgct cggcgtgccc
     2041 tctgacctca tcgccgagca cggggcggtg agcgcgcccg tcgccgaggc gatggccgag
     2101 ggcgcgcgcc tgcgcgccga cacggacttc ggtctctccg tcaccggcat cgccgggccg
     2161 ggcggaggga gcgaggagaa gccggtcgga ctcgtctaca tcgctctgtc tgacgacgcg
     2221 cacaccgagc accgtcggct tctgctcccc ggcgaccgcc aactcattcg ctggcgcgcc
     2281 gcacaggccg cactcgacct cctgcgccgg aggctgatat gaaggagtct ccgtcacgcc
     2341 acagtaccgc gagcggtagc gagcgggtgc ggcctgcgac cagagtcccg ttgcgcgccc
     2401 gcgagacccg ctcgctaccg ctcgcggtac tgtggcgtga cggagactcc ttcatatcag
     2461 cc
//
LOCUS       DASVES010000117         7126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_822293, whole genome shotgun sequence.
ACCESSION   DASVES010000117 DASVES010000000
VERSION     DASVES010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822293"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF240_04265"
     CDS             complement(<1..228)
                     /locus_tag="VF240_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174481.1"
                     /translation="MPAPFDAPEAFFPPFFAPPADAFADFFADFFADFFAAFFTDGVA
                     DFFADFFATVLPRAARADFLALAASASRRCSA"
     gene            470..652
                     /locus_tag="VF240_04270"
     CDS             470..652
                     /locus_tag="VF240_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174482.1"
                     /translation="MTDEGREKTAGGADKASTQGAAARDNQEHELEAEQESAAAMDSD
                     VPQPPDNSQTTEGQPS"
     gene            854..2404
                     /locus_tag="VF240_04275"
     CDS             854..2404
                     /locus_tag="VF240_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174483.1"
                     /translation="MALALFVLFALPLAPAFAQKLPSPERIVGDYAKAVGGRKRLAAV
                     RDATYEFSVRDDTGAESRARMLTKAPSSVRAELHTGGVEVISASNGRLAWTRDPVSGV
                     QTLTGVEAHTAKLQAMLGAGRMLDFKKKGVLARTVGVEQVGGEQAFVVEFSRREGGRV
                     RHWFGTTSKLLLKSANPLDAGAYLYDDYRPAAAGGVLEPHRSAHVTNGKTDATYTLQS
                     VRYNTGLADSLFDPPAEAGLDVSALLRELARNQKETDERVNDYTFTRKVTEREVNDRG
                     ELKKETVNVYEVYPVADHGWVLKHVAENGAPLAPERAAKEEKRVAAELAKAEREAPKR
                     AEKRERERARRDAKRRQVAKGDGEIDDAEGDDVGISTFLRVCEFISPRRERFGGRDTI
                     VFDFRPRPGYKPASRGESIVSKLAGAIWIDPAEHQVMRLDGRLIDDIKVGGGLVASVK
                     PGSAFVFEQARTAEGVWLPRFAQVNLSAKVFLFAGLTVNETHEYDDYKLFSTKSGDAA
                     LDAPKGKP"
     gene            2583..3938
                     /gene="trmFO"
                     /locus_tag="VF240_04280"
     CDS             2583..3938
                     /gene="trmFO"
                     /locus_tag="VF240_04280"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX7174484.1"
                     /translation="MKEIVVIGGGLAGVEAAWQAAQAGARVRLYEMRPVQPTPAHRTD
                     KLAEIVCSNSLKSDEPGTAPYLLKEELRRGRSLVMEAAAETRVPAGAALAVDRERFSE
                     FITARIEAHPSITLVREEVTSVSADAISIIAAGPLCSDALAEEIARLTGGDGLYFYDA
                     IAPIVAADSIDMGVAFRAARYDKGGDDYVNCPMDEEQYARFYGALVEAKSVPLQRFEE
                     TRWFESCLPIEEMARRGVDTLRYGPMKPVGLRDPRTGREPYAAVQLRQENLMADAYNL
                     VGFQNHLRFGEQARVLRLIPGLEQAEFLQYGQIHRNTYICAPRVLAPTMQMRERPEVF
                     FAGQISGVEGYVESVATGWLAGLNAARLARGAELLQAPPKTATGALARYVSGADTKNY
                     QPVNITFALLEPLAEDERRRVKKKRDRHVLQVELALREWDDFLRSIDELGQPPPRTAS
                     V"
     gene            3995..5146
                     /locus_tag="VF240_04285"
     CDS             3995..5146
                     /locus_tag="VF240_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174485.1"
                     /translation="MRITLALILLAALPAAAAWPSGAQSGRRKPPAPSPSPSGRTRTV
                     ARSPAPPSQENSPTPTPTPAATTPTTAGSVVVADDTNAPPARPRPTPAPTPAPSTKTD
                     VIDDDDEVVRISSNLVPVPATVVDERGRAVVDLELKDFELKVDGTVRPIGDLSRSETP
                     VALALLFDNSSSIRPAREFEKKAAVAFFRHVMRPIDRAAVFSISTDPEMTLGLTSDVR
                     ALVRAIESFNKPEGATALFDTVALAAEYLRPHRGRKVIVVVSDGTDTISSRSFDETLA
                     RVLAADCQVYSVQTGNSDNANLRDLAGERRLEEFAANTGGSVHAPRATSEFESAFSQI
                     AADLAQQYVLGYYPPEELRDGKFHTLTVSVATRRSLRVRTRRGYYSRKS"
     gene            5305..6231
                     /gene="xerC"
                     /locus_tag="VF240_04290"
     CDS             5305..6231
                     /gene="xerC"
                     /locus_tag="VF240_04290"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX7174486.1"
                     /translation="MIERLFEQFLEHLKYERNVSEHTLRNYAIDLGQFLEHLAPADPA
                     SGARREIDVKQIDHITIREWLSALHAAQKKKSSIARKLAALRTFFQFLIREGAVELNP
                     AKLVSTPRLERKLPNHLSVEDAVRFIETPDTETDLGNRDRAILEFLYGTGVRVSELTK
                     LDLRDIDFRNKTVRVTGKRRKERIVPFGDPALHALMRYLTEVRNKFLDNAPVAERETG
                     AVFLNYQGTRITTRSVGRMVDKYIDTCAGIHNISPHSLRHSFATHLLDSGADLRDIQE
                     LLGHARLSTTQIYTHVSMEKLIEVYDKAHPKA"
     gene            complement(6359..6739)
                     /locus_tag="VF240_04295"
     CDS             complement(6359..6739)
                     /locus_tag="VF240_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX7174487.1"
                     /translation="MDDKKIERFRTTLLEQLRQHSDQVRDNQQAALDMVAADDGVKDS
                     VDMSLHDVNQEIALRLGERESQMVADIDQALMRIDEGSYGTCAHCGKEIPERRLEALP
                     TARFDAECQSAIEANTGEDEHATL"
BASE COUNT         1335 a         2398 c         2260 g         1133 t
ORIGIN      
        1 ggccgagcag cgccgggaag cggaagctgc caaggccaga aagtccgcgc gcgcggcccg
       61 cggcaagacc gttgcgaaga agtctgcgaa gaagtccgca acgccgtcgg taaagaaggc
      121 ggcaaagaaa tctgcgaaga aatctgcgaa gaagtctgca aaggcgtctg cgggtggtgc
      181 gaagaagggc gggaagaagg cttccggggc gtcgaagggc gcgggcaagg gtcgcggcgc
      241 ggcgggggcg aagaaaggcg cgaaggccgc tgctggcgcg aagaccgggc gcgggagcgg
      301 ggggacaaag agcgtcaaga ggcaggccgg taaaaaggta acggccaagt caaacagtgg
      361 cgggccgggt cggccggggg ccggttgagg ctcgcggacg cgcacatccg ttcgcgcccg
      421 aggggcgctt cttccacgcg tgtcggtttg aatggaggtc ataaagttga tgacggacga
      481 aggtagagag aagacggcgg gcggcgccga caaagcgagc acacagggcg ccgcagcgcg
      541 cgacaatcag gagcacgagc tggaggccga gcaggagtcg gccgcggcga tggactcgga
      601 cgtgccgcag ccgcccgaca actcgcagac cacggagggg cagccctcct gaaagaccgg
      661 acaagcgcgc cggtaaactt tccagatcga aagggcgtac aactgccgaa gtttgtacgc
      721 cctttcgctt tttccgtcgc ccgcctccgc agccggcgct cttaactccc gcgcgaagca
      781 cgcagctcat atggaaaacg gaaaacgctc gccgaacacc gggccgagcc gaggcgtcag
      841 gcccggccga accttggcgc tggccctttt cgtcctcttc gcgctgccgc tcgcgcccgc
      901 cttcgcgcag aagctgccgt cgcccgaaag aatcgtcggc gactacgcga aggccgtggg
      961 cggcaggaag cgcctcgccg cggtgcgcga cgcgacatac gagttttccg tgcgcgacga
     1021 cacgggcgcc gagtcgcgtg cgcgcatgtt gacgaaagcg ccgtcctcgg tgcgcgcgga
     1081 gttgcacacg ggtggagtcg aggtcatctc cgcatcaaac gggcgcttgg cgtggacgcg
     1141 cgaccccgtc tcgggcgtgc agacgctgac cggcgtcgag gcgcacacgg ccaagctgca
     1201 agcgatgctc ggcgccggac ggatgctcga cttcaagaag aagggagtgc tggcgcgcac
     1261 ggtcggcgtg gagcaggtcg gcggcgagca ggcattcgtc gtcgagtttt cgagaaggga
     1321 aggcgggcgc gtgcgccact ggttcgggac gacgagcaag ctgctgctga agagcgccaa
     1381 ccccctcgac gcgggcgcct atctctacga cgactatcgc ccggccgccg cgggcggtgt
     1441 ccttgagcct caccgctcgg cgcatgtgac caacgggaag acggacgcga cctacaccct
     1501 ccagagcgtg cgctacaaca ccggcctcgc cgactcgctc ttcgacccgc cggccgaggc
     1561 cggtctcgac gtgagcgcgc tcctgcgcga gctggcgcgc aaccagaagg agacggacga
     1621 gcgcgtgaac gactatacgt tcacgcgcaa ggtgacggag cgcgaggtga acgaccgcgg
     1681 cgagttgaag aaggagaccg tcaacgtcta cgaggtctac cccgtggcgg atcacggctg
     1741 ggtgttgaag cacgtcgcgg agaacggcgc gccgctcgcg cccgagcgcg ccgctaaaga
     1801 agagaagcgc gtcgcggcgg aactggcgaa ggccgagcgc gaggcgccga agcgcgccga
     1861 gaagcgcgag cgcgagcgcg cccgacgcga cgcgaaaagg cggcaggtcg ctaagggcga
     1921 cggcgagatc gacgatgccg aaggcgacga cgtgggcatc tccacgttcc tgcgggtgtg
     1981 cgaatttatc agcccgcgcc gcgaacgctt cggcgggcgc gacaccatcg ttttcgactt
     2041 tcgcccgcgg cccggctaca agccggcgag ccgtggcgag agcatcgtct cgaagctggc
     2101 cggcgccatc tggatcgacc cggcggagca tcaagtgatg cggctcgacg gccgcctcat
     2161 cgacgacatc aaggtcggcg gcggcctcgt cgcctccgtc aaacccggct cggccttcgt
     2221 cttcgagcag gcgcgcacgg ccgaaggcgt ctggctcccg cgcttcgcgc aggtcaacct
     2281 ctcggccaaa gtcttcctct tcgccggcct gaccgtcaac gagacgcacg agtacgacga
     2341 ctacaagctc ttcagcacca aatccggcga cgccgcgctc gacgcaccga aaggaaagcc
     2401 gtgaatcgtg aatcgtgaat cgtcaatcgt caatcgtcaa tcgtggttga gccgccggcc
     2461 ggcttgagct acacgactca agccgaaagc ggcgtcaact ccttcgattg acgattgacg
     2521 attgacgatt cacggtcttt ttcggtttac gatttacgat tcacgattta cgattgacgg
     2581 gtatgaaaga aatcgtcgtc atcggaggcg gactcgccgg ggtggaggcc gcgtggcagg
     2641 ccgcgcaggc cggcgcgcgc gtgcgtctct acgagatgcg gcccgtacaa cccacgcccg
     2701 cgcaccgcac ggacaagctg gccgagatcg tctgctcgaa ctctctgaag tcggacgagc
     2761 cgggcaccgc tccctacctg ctcaaagagg agttgcggcg cggacgctcg ctcgtgatgg
     2821 aggctgccgc tgagacccgt gtcccggcgg gcgcggcgct cgccgttgac cgcgagcgct
     2881 tcagtgagtt catcaccgcg cgcatcgagg cgcacccgtc catcacgctc gtgcgcgagg
     2941 aggtgacaag cgtgagcgcc gacgccatct ccatcatcgc cgccggcccg ctgtgctccg
     3001 acgcgcttgc cgaagagatc gcccgcctga ccgggggcga tggcctctac ttctacgacg
     3061 ccatcgcgcc catcgtcgcg gccgactcca tcgacatggg agtcgccttc cgcgccgcgc
     3121 gttacgacaa gggcggcgac gattacgtca actgcccgat ggacgaagag cagtacgcgc
     3181 gcttctacgg ggcgctcgtg gaagccaaga gcgtgccgct ccagcggttc gaggagacgc
     3241 gctggttcga gtcgtgtctg ccgattgaag agatggcccg gcgtggggtg gacactttgc
     3301 gctacgggcc gatgaagccg gtgggcctgc gcgacccgcg aacgggcagg gagccttacg
     3361 ccgccgtcca gctccggcag gagaatttga tggccgacgc ctacaacctc gtcggcttcc
     3421 agaatcatct acgtttcggc gaacaggcgc gcgtgctacg tttgattccg gggttggagc
     3481 aggccgagtt cttgcagtac ggacagattc atcgcaacac gtacatctgc gcgccgcgcg
     3541 tgctcgcgcc gaccatgcag atgcgtgagc gaccggaagt gttcttcgcc ggacagatca
     3601 gcggcgtcga gggctacgtc gagtcggtgg cgacgggctg gctcgccggc ctcaacgcgg
     3661 cgcgactcgc gcgcggcgcg gagttgcttc aggcgccccc gaagacggcc acgggcgcgc
     3721 tcgcgcgcta cgtctccggc gccgacacga agaactatca gccggtcaac atcaccttcg
     3781 cgctgctgga gccgctggcg gaagacgagc ggcggcgcgt gaaaaagaaa cgcgaccggc
     3841 acgtccttca agtcgagctg gcgctcaggg aatgggacga ctttctccgc tccatcgacg
     3901 aactgggtca accgccgccg cgcacggcct ccgtctgacg cggcgacaga cacgcgcccg
     3961 acgtagaatc tcgcgcgccg ctatcattaa aaacttgcgt atcaccctcg cccttattct
     4021 tctcgccgcg cttccggccg ccgccgcgtg gccctccggc gcgcagtccg gccgcaggaa
     4081 gccgcccgcg ccctccccgt cgccttccgg gcgcacacgc accgtcgccc gctcgcccgc
     4141 gccgccatct caggaaaatt cgccgacgcc gacacccacg ccggccgcga cgacgccgac
     4201 cacagcaggc agcgtcgtcg tcgctgacga cacgaacgcg ccgcccgcac gcccgaggcc
     4261 gacacccgcg ccgacgcccg cgccttcaac caagaccgac gttattgacg acgacgacga
     4321 ggtcgtccgc atcagctcga acctcgtccc cgtccccgcg accgtggtcg atgaacgtgg
     4381 acgcgccgtc gtcgatctgg agttgaaaga cttcgagctg aaggtggacg gaaccgtcag
     4441 gcccatcggc gacctcagcc gctcggagac gcccgtcgcc ctcgcgctcc tcttcgacaa
     4501 cagctccagc atccgcccgg cgcgcgagtt cgagaagaag gcggccgtcg ccttctttcg
     4561 ccacgtgatg cgccccatcg accgcgccgc cgtcttctcc atctccactg acccggagat
     4621 gacgctcggg ctgacctccg acgtgcgcgc gctcgtgcgc gccatcgaaa gcttcaacaa
     4681 gccggagggc gcaacggccc tcttcgacac ggtcgcgctc gcggccgagt atctccggcc
     4741 gcaccgcgga cgcaaggtga tcgtcgtcgt ctcggacggc acggacacga tcagctcccg
     4801 cagcttcgac gagacgctgg cgcgcgtgct cgcggccgac tgtcaggtct actccgtgca
     4861 gaccggcaac agcgacaacg ccaacctgcg cgacctcgcc ggcgagcgcc gcctcgaaga
     4921 gttcgccgcc aacaccggcg gctccgtcca cgccccgcgc gcgacctccg agttcgagtc
     4981 ggccttctcg cagatcgccg ccgacctcgc ccaacagtac gtcctcggct actatccgcc
     5041 cgaggagctg cgcgacggca agttccacac cctcaccgtc agcgtcgcca cccgccgctc
     5101 cctccgcgtc cgcacccgcc gcggatacta ttctcgtaaa tcgtaaatcg tgaatcgtgg
     5161 ttgagatggc gaccggcttg agtagtgcaa ctcaagccga agcggcgtca acctcatcga
     5221 ttcacgattt acgattcacg gcttttgacc attcacggct tttttctatt tacgatttac
     5281 gatttacgat ttacgattca acccatgatc gaacgtctct tcgagcagtt cctcgaacac
     5341 ctgaagtatg agcgcaacgt gagcgagcac acgctgcgca actacgccat cgacctcgga
     5401 cagttcctcg aacacctcgc gcccgccgac ccggcttcgg gcgcgcgccg cgagatcgac
     5461 gttaagcaga tcgaccacat caccatccgc gaatggctct cggccctgca cgccgcgcag
     5521 aagaagaagt cgtccatcgc ccgcaagctc gcggcgctga gaaccttctt ccagttcctc
     5581 atccgcgagg gcgccgtcga actcaacccg gcgaagctcg tctcgacgcc gcgtcttgag
     5641 cgaaagttgc cgaaccacct ctcggtcgaa gacgccgtcc gcttcatcga gacgcccgac
     5701 accgagaccg atctgggaaa tcgcgaccgc gccatcctcg aattcctcta cggcacgggc
     5761 gtgcgcgtct ccgagctgac caagctcgat ttgcgcgaca tcgacttccg caacaagacc
     5821 gtgcgcgtca cgggcaagcg tcgcaaggag cgcatcgtcc ccttcggcga cccggcgctc
     5881 cacgcgctca tgcgctacct caccgaagta cgcaacaagt tcttagacaa cgcccccgtc
     5941 gccgagcgcg aaacgggcgc cgtcttcctc aactatcagg gcacgcgcat caccacccgc
     6001 tccgtcggcc gcatggtgga caagtacatc gacacctgcg ccggcatcca taacatcagt
     6061 ccacactccc tgcgccactc cttcgccaca cacctcctcg acagcggcgc cgacctccgc
     6121 gacattcagg aactcctcgg ccacgcccgc ctctccacga cgcaaatcta cacccacgtc
     6181 tccatggaga aactaatcga ggtctacgat aaggcgcacc cgaaagcata gaggtcagag
     6241 gtcagaggct agaggttagt aaaagcaaga tgaagaggcc gcaggtcgtg ctcggaacgc
     6301 gacctgcggc ctcttgtttt ataactaacc tctagcctct gacctctgac ctctgccttt
     6361 agagcgttgc gtgttcgtct tcgccggtgt tggcttcgat ggcggactgg cactcggcgt
     6421 cgaagcgtgc ggtggggagg gcttcgaggc ggcgctcggg aatctctttg ccgcagtggg
     6481 cgcaggtgcc gtagctgccc tcgtcgatac gcatcagcgc ctgatcgatg tcggcgacca
     6541 tctgcgactc gcgctcgccg aggcggaggg cgatctcctg attaacgtcg tggagcgaca
     6601 tatcgacgct gtccttgacg ccgtcgtcgg ccgcgaccat gtcgagggcg gcctgctggt
     6661 tatcgcgcac ctgatccgag tgctggcgca gttgttcgag gagcgtcgtg cggaagcgtt
     6721 cgattttctt gtcgtccatt agattagtct ttccccctgg gtttattccg tccggtcgtt
     6781 ttcgggagcg cggattataa cccatgccct cggggttagc acaacgtagc cggaaagttt
     6841 ctcgcccgga gcaattaaga gggaacacgg attaaacgga tgagtacgga ttcgtgttaa
     6901 tccgtgctca tccgtttaat ccgtgttccc cgaccgcgcg gcgacgctca accctacttg
     6961 caatcgacat ccactctaaa acctgaactt cagtccgccc tgcaagagcc gcgggctgct
     7021 cggcaggagg ccgcgtgtgc ggtcaacgac gtaggtgcgg tcgaagagat tcttgaccgt
     7081 gacgaagaag gtcgttcgca ggtgctcgac gcgatagttg acggtc
//
LOCUS       DASVES010000118         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1646734, whole genome shotgun sequence.
ACCESSION   DASVES010000118 DASVES010000000
VERSION     DASVES010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646734"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VF240_04300"
     CDS             <1..699
                     /locus_tag="VF240_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7174488.1"
                     /translation="DKREGVYRGIEREVRAGRQVYVVYPLVEESEKMDLKDATRMYEH
                     LRDRVFPNFAVGLLHGKMKTQEKDEVMRRFVAGEVQILVATTVVEVGVDVPNASLMVI
                     EHAERFGLSQLHQLRGRVGRGAEQSYCILLASDKQTSVARERLGIMEETTDGFRIAEK
                     DLEIRGPGEVMGTRQSGVPTFRVGNIVRDIEILEEARREAERYLTERRRTRETSRLIE
                     RVRADARFGLAAIG"
     gene            923..1504
                     /gene="rsmD"
                     /locus_tag="VF240_04305"
     CDS             923..1504
                     /gene="rsmD"
                     /locus_tag="VF240_04305"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX7174489.1"
                     /translation="MRVIAGQFRGRVLKSPPSMQVRPTSDRLRETLFNVIAPRLGGAR
                     FLDLCAGTGAVGIEALSRGASFVTFVDRVHRICGLVEANLDLCGVPEELTEVVKGDAA
                     EFLRRTVKGGGDDWRGGHGPWDIVYFDPPYAGDYLTVLDLFGEHAGALLAEGGSLIVE
                     HHHKNGLKDSVGQLRRWRILKQGDSALSFYEAQ"
     gene            1530..1952
                     /locus_tag="VF240_04310"
     CDS             1530..1952
                     /locus_tag="VF240_04310"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7174490.1"
                     /translation="MSERGSVGGRERRLSPRFNASLGCQISLPDEERSSVVLFPDAKI
                     AGRTRDLSERGLGVVAPTIYVGYDCVVDQGRTLIVSLELPSGAAIELKATAAHYIRQD
                     DSGGESSYLIGLSITGMDDDNRSRYTTLLAELAGPGKH"
     gene            2037..>2594
                     /locus_tag="VF240_04315"
     CDS             2037..>2594
                     /locus_tag="VF240_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174491.1"
                     /translation="MIHTLKCPSCAAPVEFEDETDRRTFRCPFCNNTVVIPDSMRSRE
                     HEEPQIVVSTYAYRPGRTIRVKPGSVIIPILAVIVIFVGIAFAAVYFSVRSFQRGVER
                     TVAGVANVPNIPNIPSFGPGKSTPASKTGSSFATVVLQFGKEGTGPGYFKDARSIAVD
                     GAGRIYVGEYSGGRIQVFDGAGKFIT"
BASE COUNT          447 a          867 c          845 g          435 t
ORIGIN      
        1 gacaagcgcg agggcgtcta ccgcggcatc gaacgcgagg tgagggcggg ccggcaggtc
       61 tacgtcgtct acccgctcgt cgaggagtcg gagaagatgg acttgaagga cgccacgcgg
      121 atgtacgaac acctgcgcga ccgcgtcttt ccaaacttcg cggtcggctt gctacacggc
      181 aagatgaaga cgcaggaaaa ggacgaggtg atgcgccgct tcgtcgcggg agaggtgcag
      241 atactcgtgg cgaccacggt cgtcgaggtc ggcgtggacg tgccgaacgc ctcgctgatg
      301 gtcatcgagc acgccgaacg tttcggtctc tcgcagctcc accagttgcg cgggcgcgtc
      361 gggcgcgggg cggagcagtc atactgcatc ctgctggcgt cggacaaaca gacctcggtc
      421 gcgcgcgagc gcctcggcat catggaggag accaccgacg gcttccgcat cgccgagaag
      481 gatttggaga tacgcggccc tggcgaggtg atgggcacgc gtcagtcggg cgtccccacg
      541 tttcgcgtcg gcaacatcgt ccgcgacatc gagatactcg aagaggcgcg gcgcgaggcc
      601 gaacgatatc tcacggaaag acggcgcacg cgtgagactt caagactcat cgagcgcgtg
      661 cgcgccgacg cgcgtttcgg tctggccgcc atcggctaaa ctctcccaac agacggccgt
      721 ccgccgatag tgtcccaagt tgaatgtttt cctctgcgcc ccctcggcgt cctttgcggc
      781 tcggcggtga atgagcgccg gggttgactc accgccgagt cgccgaggac gctgaggacg
      841 cgctgagaac tgtaatgagg acactattcg tccgccacgg cccggtgttc aaaatctcgc
      901 cgtgtgctaa attcaatttc ttatgcgcgt catcgccgga cagttccgcg ggcgggtgtt
      961 gaagagtccg ccgtcgatgc aggtgcgccc gacctccgac cgtttgcgcg agacactctt
     1021 caacgtcatc gcgccgcgtc tcgggggcgc gcgcttcctc gacctgtgcg ccggcacggg
     1081 ggccgtcggc atcgaggcgc tctcgcgcgg cgcgtcgttc gtcaccttcg ttgaccgcgt
     1141 ccaccgcatc tgcggactgg tcgaagccaa cctcgatctg tgcggcgtgc ctgaggagtt
     1201 gaccgaggtc gtcaaaggcg acgccgcgga attcttgcgg cgcaccgtca aaggcggcgg
     1261 cgacgactgg cgcggcggcc acggcccgtg ggacatcgtc tacttcgacc cgccctacgc
     1321 cggcgattac ctcacagtcc tcgacctctt cggcgagcac gcgggcgcgc tcctcgccga
     1381 gggcggaagt ctcatcgtcg aacaccatca caagaacggt ctgaaagact ctgtcggcca
     1441 gctccgccgt tggcgtatac tgaagcaggg cgattcggct ctgagcttct acgaagcaca
     1501 ataaacccga ccggaggcgg agtcgtcaga tgagcgagcg tgggagtgtg ggtgggcgcg
     1561 agcgacgcct ttcgccgaga ttcaacgcga gcctcggctg tcaaatcagt ctgccggatg
     1621 aggagcgcag ctcggtcgtg ctcttccccg acgcaaagat tgcgggccgc acgcgcgacc
     1681 tgagcgagcg cgggctgggc gttgtcgcgc ccacgatcta cgtgggctac gactgcgtcg
     1741 tcgatcaggg ccgcacgctc atcgtctcgc ttgagcttcc ctccggggcc gccatcgagc
     1801 tgaaggccac ggccgcgcac tacatccggc aggacgacag cggcggcgag tcgagctacc
     1861 tgatcggcct cagcatcacg gggatggacg acgacaaccg ctcccgctat accacgctcc
     1921 tcgctgaact ggccggcccc ggcaagcact gacgcgcggc cgaccacctt cttcatctcc
     1981 actgccgcgc gcgcggccct tgcacggggc ggcccggcag ttgataaaat gcgccaatga
     2041 ttcacaccct caagtgcccc tcctgcgccg cgcccgtcga gttcgaggac gagaccgacc
     2101 ggcgcacgtt ccgctgcccc ttttgcaaca acacggtggt catccccgac tcgatgcgaa
     2161 gccgtgagca cgaggagccg cagatcgtcg tctccaccta cgcctaccgg ccgggccgca
     2221 cgatccgcgt caagccgggg tcagtcatca tccccatcct cgcggtcatc gtcatcttcg
     2281 tcggcatcgc cttcgcggcc gtctacttca gcgtccgcag cttccagcgc ggcgtggaac
     2341 ggacagtggc cggcgtcgcc aacgtcccca acatccccaa catcccgtcg ttcgggccgg
     2401 ggaagagcac gccggcttcc aagaccgggt cgtccttcgc cacggtcgtg ctccagttcg
     2461 gcaaagaggg gacggggccg ggctacttca aggacgcgcg cagcatcgcc gtggacggcg
     2521 cggggcgcat ctacgtcggc gagtattcgg gcgggcgcat tcaggtcttc gacggggccg
     2581 gcaagttcat cacg
//
LOCUS       DASVES010000119        13844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_423013, whole genome shotgun sequence.
ACCESSION   DASVES010000119 DASVES010000000
VERSION     DASVES010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423013"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..441
                     /locus_tag="VF240_04320"
     CDS             271..441
                     /locus_tag="VF240_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174492.1"
                     /translation="MKKESGAEVEMSRGGLGELSVCIDGRKVVETRRFWFASPGSVVS
                     RVRAALDGRDDG"
     gene            491..886
                     /locus_tag="VF240_04325"
     CDS             491..886
                     /locus_tag="VF240_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7174493.1"
                     /translation="MDAKTGKKTIEEGLSLLPGPGGERFVQVFEHGSLTVEVYAPRGR
                     DPQQPHTRDEVYVVARGRGEFVSADGERVSFEPNDFLFAAAGVAHRFENFSDDFAVWV
                     LFYGPEGGEVEHGDDASEDNSGDEAGEKS"
     gene            901..1659
                     /locus_tag="VF240_04330"
     CDS             901..1659
                     /locus_tag="VF240_04330"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEX7174494.1"
                     /translation="MRTTEAKRLAATRRLLPAALLLAAVGCAAPSTATQNAAGAASPK
                     ASATQGAAQAGAQQPQQGASSGSAFLDRLVAATVERTHHEVRYDPTYFRIAYPGGDVP
                     EGVGVCTDEVIRPYRAVGVDLQREVHEDMARNFRLYPKKWGLSRPDTNIDHRRVPNLM
                     VFFSRKGASLPVTDRAADYRPGDVVTWDLGGGLTHIGIVVGTPSAADPARFQIVHNIG
                     AGPKMEDVLFGWKITGHYRYEGPGARPLSRAREE"
     gene            1662..2102
                     /locus_tag="VF240_04335"
     CDS             1662..2102
                     /locus_tag="VF240_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174495.1"
                     /translation="MTITLRDVTRDNWIQCTKLELREDQKGFVAPNVFSIAEWRFEPT
                     FTPQAVYHDEEMVGFIMYGYDTEEGCHWVGRMMIDKSQQGRGYGRAAMVEAIRRMREQ
                     PDCRQIGLCVEPTNAAAQKLYESLGFAKTGEVSHGEDVMRLRFE"
     gene            2295..3362
                     /gene="rsgA"
                     /locus_tag="VF240_04340"
     CDS             2295..3362
                     /gene="rsgA"
                     /locus_tag="VF240_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX7174496.1"
                     /translation="MQLEKYGWNDFFVEGFAPYAAEGYGVGRILLQHNKIYVLYTEDG
                     EMEAEVTGRLRHEAAGPHELPAVGDWVATRERGTDGTLMIHAVLPRSSKFSRKVSGSR
                     TDEQVVAANVDTVFLVAGLDGDYNPRRIERYLIMAWESGARPIVVLNKSDLDPAGAAE
                     RAVEVEAVAPGVAIVLLSAKHDEQLAQLAPFVGPGRTVALMGSSGVGKSTIVNRLAGS
                     DVQRTAEVRVGDSRGRHTTTHRQLILLPGGGLVIDTPGMRELQLLVSERGMRETFEEI
                     ETLAAQCRFTDCRHAEERDCVVRAALADGTLDAERYANYQKMLGEMRHTAVRQDQRAA
                     QEEKSRVKRLTRAFNKTDKRR"
     gene            3428..4429
                     /locus_tag="VF240_04345"
     CDS             3428..4429
                     /locus_tag="VF240_04345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7174497.1"
                     /translation="MKISVVIPVRNEEDSIRTLLDCVLGQTRMPDEVVVTDGGSTDKT
                     PSIIEEYVRRGAPVVLIREAAALPGRGRNVAAARASFDWLAFADAGTRPAPGWLEALA
                     ERVEHDPTVEVVYGNWESITDTFFKECAAIAYVHVTPIEVEGATIPPPWIASALIRRA
                     VWSAVGGFADDLRSAEDLVFQEKIKEGEFRVAYAPRAVVGWTMQPTLWRTFKRFVTYS
                     RHNLRAGLGGQWQSAIITRYALLTLCALPALFFGLRWLVVPAALWLLMLAARAVVALH
                     RNRVAYPASVRRNLLRLLLLVPLLATIDAATILGALHWLAADKLRPGAAPAGAGGGA"
     gene            complement(4444..5217)
                     /locus_tag="VF240_04350"
     CDS             complement(4444..5217)
                     /locus_tag="VF240_04350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174498.1"
                     /translation="MENQKVLDAVNKKTYARGDVVKHYENLDMLHKPERMLLERLTPE
                     IKDKKILDIAIGGGRTTKYLLQISKDYTGIDYTPEFVEVVKRKYKDATILCCDARDLK
                     EFADETFDFVLFAFNSIDYIPHQDRIKALSESYRVLKKGGYFMFSTHNRDHESFNKLP
                     WQEKFQFKLSYLKYCLYCLYHLPKHREMKKHEVHTDEFAVINDNAHGFSLLAYYISIP
                     EQLKQLAAVGFSDVEVYDGEGERVTSDTRFPWTHYLAKR"
     gene            complement(5291..6061)
                     /locus_tag="VF240_04355"
     CDS             complement(5291..6061)
                     /locus_tag="VF240_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174499.1"
                     /translation="MIVFKKKFIRIAEAWGGEEPAAEDVDLLRCFQRTEPAADFLCRE
                     FYTIIIDLTQEPDALFAALRKGCQYKVRRAEARDNFVYECWNGAGQSDRERDALARFS
                     ELYDWFAARKRLPKIDRAWLSLMAETGRLDVSCARERTGEALVWHIHYRSGGKATFLH
                     SASLLPDLEGEERSRLGRASRFHHWRDMLRFKSEGMSVYDFGGWYQGSEDTARLGINK
                     FKEEFGGTVVKNYICERPLTLRGKLFLQLRQLLLGDAI"
     gene            complement(6080..6982)
                     /locus_tag="VF240_04360"
     CDS             complement(6080..6982)
                     /locus_tag="VF240_04360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7174500.1"
                     /translation="MKNFVFALLHAAGVTRFAAWWNRKRVIFLCYHGVTERATRGPHD
                     PHGIHVRHHLFEAQLDYLRRHYHVTTLREYLAARHDGRSLPDYSVVLTFDDGFRNFLT
                     AAAPRLAARGIPASLFIITDNAAEGDATRIERRWQPSDDVTYLSWAEAQTLEREQGIE
                     IGSHTCSHPRLLTLSPEETERELRESYEAIVAHMGHTAPAFSYPKGEYSQMLGVQARA
                     MGYACAVTVDGGANDADADLFALGRTLIGDDDDKASFAVRVSGLRNWIAELRTILSGG
                     SVASKRRELRKRVPLSVSEPSKNP"
     gene            complement(7401..10424)
                     /locus_tag="VF240_04365"
     CDS             complement(7401..10424)
                     /locus_tag="VF240_04365"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7174501.1"
                     /translation="MRLQRLLAAVVAVVLLTAGLPALAQKSKRPKPAAAVAPGASRLD
                     LKVNEFTLPNGLRVVLHEDHSAPIVAVNLWYHVGSKNEVPGKTGFAHLFEHMMFQGSK
                     NFDYDYFFPLQEAGGNINGSTNPDRTNYYEVVPSNFLETALYLEADRMANLLDAMTEE
                     KLANQRDVVKNEKRQNYDNRPYGLVGAKIAAATYPPEHPYHWLTIGSLDDLTAASMDD
                     VKDFFRRFYVPNNVSLTIAGDFQPAEARRLVEKHFGSVARGPEVKRVAARQPVMEREM
                     RMQEEDSVALPRVYMVWHTVPAFSPDDAALDMLATVLSSGKGSRLYKRLAYDQQIAQE
                     VFASHPSREIAGQFNVVATAKPGKALADIEKMIDEEVAKLKAAPPTREEVERAYNEYE
                     ASSVYGIQTVLGKADQLNQYATYRNRPVYFQQHLDAYRKVTAADIRRVANKYLTDKRL
                     VLTVVPRGRQNKTGEPVAGAPKGEAPAPATQTAAGAAPRTTAAVTGERPAGAQTQTKT
                     EAAQTRPAGSAPPTAAAPATPPQKKESTRPDTSRLPKPGPEPSLALPRLERRTLSNGL
                     EVLVVQHHELPVVNMNLVLKSGGEADPADRAGLASMTAGLLDEGTRTRSALDISNALG
                     SIGAALNTSAGWDSSGANLLTLTRHLDRALDIYSDVILNPSFPDDEMKRLRDQRLTNL
                     KQRRDDANAIAGVVYPALIYGRSHPYGHSLLGDETSVAAVTTADVRKFYETYYRPNNA
                     ALVVVGDVTPEQIVPKLERAFAKWERGHVPAVDVTAAAVRRERNTIYVVDRPGAAQSV
                     VQIGHIGVPRSTPDYFALALLNRLLGGAFISRVNLNLRENKGYTYGARTSFDYRRGAG
                     PFMASAGVQTAVTKESVAEFMKELRGVRGDMPITPEELQYARQSFARGFVGGFETPAQ
                     IAARLEDVVIHGLPDDYFNNFTARVGAVTLEDVTRVAQQYLNPERFAILVVGDRRVIE
                     KGLRELEDVSNDIVFIDADGRPVAPMGGAPSGTQR"
     gene            complement(10785..10982)
                     /locus_tag="VF240_04370"
     CDS             complement(10785..10982)
                     /locus_tag="VF240_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174502.1"
                     /translation="MDDRQRAYIVIALTAGAGKKHEDCKVIPHGDVYPAIYTQVFGPA
                     SQRDCEKWMNANCGKSDKATR"
     gene            11182..12798
                     /gene="nadB"
                     /locus_tag="VF240_04375"
     CDS             11182..12798
                     /gene="nadB"
                     /locus_tag="VF240_04375"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX7174503.1"
                     /translation="MLETDYIIVGSGIAGLRAAIELARAGARVTVLTKDRPEESNTEY
                     AQGGIAVALAEDDEASLHEDDTAAAGAGLCDERAVSVLVEDGRAGVRELIGWGAEFDR
                     EGGRLMFTQEAAHSRRRILHAGGDATGHEIGRALIARAGHERNLSFVPHAATESLIVE
                     DGRVGGVRYLDPLVRRAREMRARGVVLASGGAGQLFLHTTNPEVATGDGMAMAYLAGA
                     ELADMEFVQFHPTALNVAGAPRFLLSEALRGEGGVLRNAEGRRFMPRYHERAELAPRD
                     VVSRSIVAEMERTATRTVYLDMTGLEARFVRSRFPVIYSTCLRFGLDLTREPVPVSPA
                     AHYIMGGVRTDLDGRTTLPGLYAAGEVACTGVHGANRLASNSLLEGLVFGARAGRAAA
                     REFADKPAPRRNLTARRDAEETATAEHHSEEQGRAGAAALAVRKRVRRLMWERVGILR
                     SRNSLERALREFEEIGRAPLRPGPRNFLTVATLVARSALWREESRGAHHRVDFPERDD
                     ARWRRHSIIQRDGGVSSSETVEFAATVEKL"
     gene            13010..13759
                     /gene="nfi"
                     /locus_tag="VF240_04380"
     CDS             13010..13759
                     /gene="nfi"
                     /locus_tag="VF240_04380"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEX7174504.1"
                     /translation="MAQYEKLHDWDLKPREAIELQKRLRERVEVVPLKRKVETIAGAD
                     ISFNKFEPTVYAGLVVLRLPTLEVVEEVGVVSETKFPYVPGLLSFRETPSLLEAWEKL
                     KVEPDAVMFDGQGLAHPRRVGIASHVGLMLKRPTLGCAKSVLVGKFEEPGEERGSWSP
                     MTDKGETVGAAVRTKTRVQPVYVSPGHLIDLEGAIDLVLRTDGGYRQPEPTRRAHLLV
                     NALRRGERAPTSEEDAGRKDAGDASQGAFQF"
BASE COUNT         2307 a         4352 c         4763 g         2422 t
ORIGIN      
        1 gaagcctcgc gggtgtgcct acgatgacag aaccgccccg cgcaagcgag acccgcgagg
       61 cttccgctcg cggtactgtt tgatgtgctc acgctggagg cgcaaagccg gcaggctcag
      121 gggggagttg taatctttta caaacaaccg cgcgcggagt tcgtattatc gtctcgacga
      181 gggagacgta acgccatgcc gcggataacg attcgctact gcccgctttg actggagaaa
      241 tggtttgtcg ctgcccgtgt ggcggcgcgt ttgaagaagg agtcgggcgc ggaggtcgag
      301 atgagccggg gcggactcgg cgaactgagc gtttgcatcg acggtcggaa ggtcgtcgag
      361 acgcggcgct tctggttcgc gtcgcccggc tcggtcgtct ctcgcgtgcg cgcggcgctc
      421 gacgggcgcg acgacggttg agtcggcccc ggccgtttac ctcgggcgca acacggagga
      481 taatttctcg atggacgcga agacggggaa gaagacaatc gaggaagggc tgtcgttgct
      541 gccggggccg gggggcgaac gcttcgtgca ggtcttcgag catgggagcc tgacggtcga
      601 ggtctacgcg ccgcgcgggc gtgacccaca gcagccgcac acgcgcgacg aggtgtacgt
      661 ggtggcgcgt gggcgcggcg agttcgtgag cgccgacggc gagcgcgtct ccttcgagcc
      721 gaacgatttt ctattcgccg ccgcgggcgt cgcgcaccgc ttcgagaatt tctctgacga
      781 cttcgccgtg tgggttcttt tctacggccc cgagggcggg gaggttgagc acggcgacga
      841 cgcgagcgaa gacaactccg gcgacgaggc cggcgagaaa tcttgagtga ttatttgaag
      901 atgaggacga cggaagctaa acgcctcgcc gcgacccgcc gcctgttacc ggcggcgctg
      961 ctgctggctg ccgtcggttg cgccgcgccg tctacggcga cgcagaacgc cgcgggcgca
     1021 gcgtcgccga aggcgagcgc gacgcagggc gcggcgcagg cgggcgcgca gcagccacag
     1081 cagggagctt cgagcggctc ggcgtttctc gaccgcctcg tcgccgcgac tgtcgagcgc
     1141 acgcatcacg aggtgcgcta cgacccgacg tatttccgca tcgcctatcc gggcggcgac
     1201 gtgcccgagg gcgtgggcgt ctgcacggac gaggtgattc gcccctaccg cgccgtcggc
     1261 gtcgatctcc agagagaagt ccacgaggac atggcgcgca acttccgtct ctacccgaag
     1321 aagtggggac tctctcggcc cgacaccaac atcgatcacc ggcgcgtgcc gaatctgatg
     1381 gtcttctttt cgcgcaaggg cgcttcgctc cccgtcaccg accgcgcggc cgactaccgc
     1441 cccggcgacg tggtgacgtg ggatttgggc ggcgggctga cgcacatcgg catcgtcgtg
     1501 ggcacgccct cggcggccga ccccgcgcgc ttccagatcg tccacaacat cggcgcgggg
     1561 ccgaagatgg aggacgtgct cttcggctgg aagatcacgg gccactatcg ctacgaaggg
     1621 ccgggcgcgc ggccgctcag ccgggcgcgt gaagagtagc catgaccatc acgctgcgcg
     1681 acgtgacgcg cgacaattgg attcaatgca ccaagctcga attgcgcgaa gaccagaagg
     1741 gcttcgtcgc gccgaacgtc ttctcgatag cggagtggcg cttcgagccg accttcacgc
     1801 cgcaggccgt ctaccacgac gaggagatgg tcggcttcat catgtacggc tacgacaccg
     1861 aagagggctg ccactgggtc gggcgcatga tgatcgacaa gagccagcag ggccgtggct
     1921 acgggcgcgc ggcgatggtc gaggccatcc gccggatgcg cgagcagccc gactgccgcc
     1981 agatcggcct ctgcgtcgag ccgacgaacg ccgccgcgca gaagctctac gagagcctcg
     2041 gcttcgcgaa gacgggcgag gtctcacacg gcgaggacgt gatgcggctg cgcttcgagt
     2101 agaggcttct ttcttgagta ttgggaaagt cgtactcacc gcagaggcgc agaggacgca
     2161 gagggggcgc cgcaggcttg acgcttacac tcggcgttgg ttgtgctgct taaccctctg
     2221 cgtagtctct gcgtcctctg cgcctctgcg gtgaactgcc gttcccatca cgggcgcatg
     2281 gagttaaaac tgacttgcag ttagaaaaat acggctggaa tgatttcttc gtcgagggct
     2341 tcgcgcccta cgcggccgaa ggctatggcg tcgggcgcat cctcctccag cacaacaaaa
     2401 tctacgtgct ctacacggag gacggcgaga tggaggccga ggtgacgggc cgcctgcgcc
     2461 acgaggcggc ggggccgcac gagctgccgg ccgtgggcga ctgggtggcg acgcgcgagc
     2521 gcggcaccga cgggacactg atgatccacg ccgtgcttcc gcgcagcagc aagttctcgc
     2581 gcaaggtgtc ggggagccgg acggacgagc aggtcgtcgc cgccaacgtg gacacggtct
     2641 ttctcgtcgc gggcctcgac ggcgactaca acccccgccg catcgagcgt tacctcatca
     2701 tggcgtggga gagcggtgcg cgccccatcg tcgtcctcaa caagtcagac ctcgacccgg
     2761 cgggcgcggc cgagcgtgcg gtcgaggtcg aagcggtcgc gccgggcgtc gccatcgtgc
     2821 tgctgagcgc caagcacgac gagcagctcg cgcagctcgc gccgttcgtc ggccccggtc
     2881 ggacggtcgc gctgatggga tcgtcgggcg tgggcaaatc gaccatcgtc aaccgtctgg
     2941 ccggctcgga cgtgcagcgc accgccgagg tgcgcgtggg cgacagccgc ggccggcaca
     3001 cgacgacgca ccggcagctc atcctcctac ccggcggcgg cctcgtcatc gacacgccgg
     3061 ggatgcgcga gctgcaactc ctcgtgagcg aacgcgggat gcgcgagacc ttcgaggaaa
     3121 tcgaaacgct cgccgcccag tgtcgcttta ccgactgccg gcatgcggag gagcgcgact
     3181 gcgtcgtccg cgcggcgctt gccgacggca ctctcgacgc cgagcgctac gcgaactacc
     3241 agaagatgct cggcgagatg cggcacacgg ccgtcagaca ggatcagcgc gcggcgcagg
     3301 aagagaagtc gagggtcaag agactgacgc gcgccttcaa caagaccgac aagcgccgct
     3361 gagcggtcgc cgggaaacag caacgcggac gacgcggcgg gccacctttc ggggcacaat
     3421 caataggatg aaaatcagcg ttgtcattcc ggtgcggaac gaagaagact cgatccgcac
     3481 gctcctcgat tgcgtgctcg gccagacgcg aatgcccgat gaagtggtcg tgaccgacgg
     3541 cggctcgacg gacaagacgc cgagcatcat cgaagagtac gtgcggcgcg gcgcgcccgt
     3601 ggtgctcatc cgcgaggcgg cggccctgcc cggtcgcggg cgcaacgtcg cggcggcgcg
     3661 cgcctcgttc gactggctgg ccttcgccga cgccggcacg cggcccgcgc cggggtggct
     3721 cgaagcactc gccgagcggg tcgaacacga cccgacggtg gaggtcgtct acggcaactg
     3781 ggagtcgatc acggacacct ttttcaagga gtgcgcggcg attgcttacg tccacgtcac
     3841 gcccatcgaa gtcgaagggg cgaccatccc tccgccatgg atcgcctcgg cgctgatccg
     3901 ccgcgcggtg tggagcgccg tcggcggttt cgccgacgac ctgcgctcgg ctgaagacct
     3961 tgtgtttcag gaaaaaataa aagaggggga gttccgcgtg gcctacgcgc cacgcgccgt
     4021 cgtcggctgg accatgcagc ccacgctctg gcggacgttc aagcggttcg tcacgtactc
     4081 aagacacaac ctgcgcgcgg ggctgggcgg gcagtggcag tccgcgatca tcacgcgcta
     4141 cgcgctgctc acgctgtgcg ccctgccggc gctcttcttc ggactccgct ggctcgtcgt
     4201 cccggcggcc ctgtggctgc tcatgctggc cgcgcgcgcc gtcgtcgcgc tccaccgcaa
     4261 ccgagtggcc tatccggcca gcgtccggcg caacctgctg cgattactac tgctcgtgcc
     4321 gctcctggcg acgattgacg cggcgacgat cctcggcgcc ctccactggc tcgcggcgga
     4381 taagctccgg cccggcgccg ctccggcggg cgcgggcggc ggggcatgag ggcgcgggcg
     4441 cggctacctc ttcgccagat agtgagtcca cgggaaccgg gtgtcgctcg tcacccgctc
     4501 gccttccccg tcataaactt cgacatcgga aaatccgacg gcggcgagtt gtttcaactg
     4561 ctccgggatg ctgatgtagt aggcgagcag cgagaagccg tgcgcgttgt cgttgatgac
     4621 ggcgaactcg tcggtgtgga cttcgtgctt tttcatttcg cggtgtttgg gcaggtggta
     4681 gaggcagtac aggcaatatt tgaggtagct aagtttgaat tggaatttct cctgccatgg
     4741 gagcttgttg aagctttcgt gatcgcggtt gtgcgtggag aacatgaaat agccgccttt
     4801 tttgagcacc cggtacgact cacttaaggc tttaatcctg tcctgatgcg ggatgtagtc
     4861 gatgctgttg aaggcgaaca ggacgaaatc gaaagtctcg tcggcaaact ctttcaggtc
     4921 tctcgcgtcg cagcacagga tggttgcgtc cttgtacttc cgcttcacca cctcgacgaa
     4981 ttccggcgtg taatctatcc ccgtgtagtc cttgctgatt tgcagcaggt atttcgtcgt
     5041 ccggccgccg ccgatggcga tgtccagaat ttttttatct ttaatctccg gggtcagcct
     5101 ctcgaggagc atcctctccg gcttgtggag catgtcaaga ttctcatagt gcttgacgac
     5161 atcgcctctc gcgtatgttt ttttattaac ggcgtctaaa actttttgat tctccatctc
     5221 attcctgctc ctcaaattta actcgtggcg gggacgcggt aagtagtcgc ggcgcgtgaa
     5281 tgcttcacac tcaaatggcg tcgcccagta gcaactggcg gagttgtaaa aacagcttgc
     5341 ctctcaacgt gagcgggcgt tcgcagatat agtttttcac aaccgtcccg ccaaactctt
     5401 ccttgaattt attgatgccg agccgggctg tgtcttcgct tccctgatac cagccgccga
     5461 agtcgtaaac gctcatccct tcactcttga accgcagcat gtcacgccag tgatgaaaac
     5521 ggctggcgcg tcccaaacgg ctccgctcct cgccttcaag gtcgggcaga agcgaagcgg
     5581 aatgcagaaa tgtcgccttg ccgccgctgc gataatggat gtgccagacg agcgcctcgc
     5641 ccgttcgctc gcgcgcacag gaaacgtcga gcctcccggt ttcagccatc agcgacaacc
     5701 atgcgcggtc aattttgggc agacgttttc gcgccgcgaa ccagtcgtaa agctcactga
     5761 agcgagcgag ggcatcccgt tcgcggtcac tttggccggc gccgttccaa cactcgtaga
     5821 caaaattatc tctggcctcg gcgcggcgga ctttgtattg acagcctttc ctcaaggccg
     5881 cgaacagcgc gtccggctcc tgtgtgaggt cgattatgat tgtataaaac tcgcggcaca
     5941 agaagtctgc cgccggctct gtgcgctgga agcagcgcaa caggtcaacg tcctccgcgg
     6001 ccggctcttc gccgccccac gcctcggcga ttcggataaa cttctttttg aaaacgatca
     6061 tggagggccg ggcgcggcct cacgggtttt tggaaggttc actgaccgag agaggcacgc
     6121 gtttgcgtag ttccctgcgc ttactggcga cgctgccgcc ggagaggatg gtgcgcagtt
     6181 cggcgatcca gttccgcagc ccggagacgc ggacggcgaa cgacgccttg tcgtcgtcgt
     6241 cgccgatgag cgtgcgcccc agcgcgaaca ggtcggcgtc ggcgtcgttg gcgcccccgt
     6301 ccaccgtcac ggcgcaggcg tagcccatgg cgcgcgcctg cacgccgagc atctgcgaat
     6361 actcgccctt cgggtaagag aacgcgggcg ccgtgtggcc catgtgcgcg actatcgctt
     6421 cgtaggactc ccgcaattcg cgctcggtct cttcgggcga gagcgtgagc agccgcgggt
     6481 gcgagcaggt gtgggagccg atctcgatgc cctgttcacg ctccagcgtc tgcgcctccg
     6541 cccacgacag gtacgtcacg tcgtcgctcg gttgccagcg acgctcgatg cgcgtcgcgt
     6601 cgccttcggc ggcgttgtcg gtgatgatga agagtgaagc ggggatgccg cgcgcggcca
     6661 gacgcggggc ggcggccgtc aggaagttgc gaaagccgtc gtcgaaggtc aggacgaccg
     6721 agtagtcggg aagagatcgc ccatcatgcc gggccgccag atattcgcgc agggtggtga
     6781 cgtgatagtg ccgccgcagg taatcaagct gcgcctcgaa gagatgatga cggacgtgga
     6841 tgccgtgcgg gtcgtgtggg ccacgcgtgg cgcgttcggt gacgccgtgg tagcagagga
     6901 agatgacgcg cttgcgattc caccaggcgg cgaagcgagt gacgccggcg gcatgcaaga
     6961 gggcaaagac gaaatttttc atcagacccg ctcaagctca gacgagtaac cgaagaggct
     7021 gaaactttcc acctcaacaa ctcaggcaaa gaatgtctgg aagggcactg atactaagac
     7081 cctggcaagg agtctacgac ggcgcctcat aaacatcgga tggcggagag catactacag
     7141 ttgagcgggc gggcgcaaaa cctttcgccc tgacaatttt aggggcgccg cgcccttgcc
     7201 aagcgcaacg ccccggcccg gtaaggaggc cccgaccggg tgaccatcag cagccctctt
     7261 tgcccagtgg cgtgccaatg tctctcatcg tttggacgcc ggaggcgctc gcgcggtctg
     7321 gaaaaacagt accgcgagcg gtagcgagcg ggtgtggtta cgcgagacac aacccgctcg
     7381 ctaccgctcg cggtagtgtt ttaccgttgc gtgccgctgg gggcgccgcc catgggcgcg
     7441 acggggcggc catcggcatc gatgaagacg atgtcgttcg acacgtcttc aagctcgcgc
     7501 aaccccttct cgatgacgcg gcggtcgccg acgacgagga tggcgaagcg ctccgggttg
     7561 aggtactgct gcgccacgcg cgtcacgtct tcgagcgtga ctgctccgac gcgcgccgtg
     7621 aagttgttga agtagtcgtc gggcaggccg tggatgacca cgtcttcgag acgggcggcg
     7681 atttgcgccg gcgtctcgaa cccgccgacg aacccgcgcg cgaagctctg ccgggcgtac
     7741 tgcaactcct cgggcgtgat gggcatgtcg ccgcgcacgc cgcgcagctc cttcatgaac
     7801 tccgcgaccg actctttcgt caccgccgtc tgcacgccgg ccgacgccat gaacggcccg
     7861 gccccgcgcc ggtagtcgaa cgaggtgcgc gcgccgtagg tgtagccctt gttctcgcgc
     7921 agattcaggt tgacgcgcga gatgaaggcc ccgccgagca ggcggttgag gagggcgagg
     7981 gcgaagtagt cgggcgtcga gcgcgggacg ccgatgtggc cgatctggac gacggactgc
     8041 gccgcgccgg ggcggtcaac gacgtagatg gtgttgcgct cgcgccgcac ggccgcggcc
     8101 gtcacgtcaa cggccggcac gtggccccgc tcccacttcg cgaaggcccg ctccagcttc
     8161 ggcacgatct gctcaggcgt cacgtcgccg acgacgacga gcgccgcgtt gttcggacga
     8221 tagtaagtct cgtagaactt ccgcacgtcg gccgtcgtca cggcggctac ggaggtctcg
     8281 tcgccgagga gcgaatggcc gtaggggtgg ctgcggccgt agatgagggc cgggtagacg
     8341 acgccggcga tggcgttcgc gtcgtcgcgc cgctgcttga ggttcgtcag gcgctggtcg
     8401 cgcaggcgct tcatctcgtc gtcggggaag gaggggttga ggatcacgtc cgagtagatg
     8461 tcgagggcgc ggtcgaggtg gcgcgtcagg gtgagcaggt tggcgccgga ggagtcccag
     8521 ccggcgctcg tgttgagcgc ggcgccgatg gagccgagcg cgttggagat gtcgagcgcc
     8581 gagcgcgtgc gcgtcccctc gtcgagcaga ccggcggtca tgctggcgag tcccgcacgg
     8641 tcggccgggt cggcctcgcc gcccgacttg agcaccagat tcatgttgac gaccggcaac
     8701 tcgtggtgct ggacgaccaa aacttcgagg ccgtttgaga gcgtgcggcg ctcaagacgc
     8761 ggcagcgcaa gcgacggctc ggggccgggc ttcggcaggc gcgaggtgtc gggccgcgtg
     8821 ctttccttct tctgcggcgg cgtggcgggc gcggccgcgg ttggcggcgc ggagcctgcg
     8881 gggcgcgtct gcgcggcctc ggtcttggtc tgcgtttgcg cgccggcggg acgctcaccg
     8941 gtgacggccg cagtcgtgcg cggcgcggcg ccggcggccg tctgcgtcgc gggcgcgggc
     9001 gcttccccct tcggagcgcc ggccaccggc tcgcccgtct tgttctgccg tccgcgcgga
     9061 acgacggtga gcaccagacg cttgtcggtc agatacttgt tcgccacgcg ccggatgtcg
     9121 gcggccgtga ccttgcgata ggcgtctaag tgctgctgga aatagacggg gcgattgcgg
     9181 taggtcgcat actggttgag ctggtcggcc ttgccgagca cggtctggat gccgtagacg
     9241 gacgacgcct cgtactcgtt gtaagcgcgc tcgacctcct cgcgcgtcgg cggggcggct
     9301 ttcagtttgg cgacctcttc gtcgatcatc ttctcgatgt cggcgagggc cttgccgggc
     9361 ttggcggtgg cgaccacgtt gaactggccg gcaatctcgc gcgaagggtg ggaagcgaaa
     9421 acctcctgcg cgatctgctg atcgtaggcg agccgcttgt agagacgcga ccccttgccg
     9481 ctggagagca ccgtcgccag catgtcgagc gcggcgtcgt ccggcgagaa ggccgggacg
     9541 gtgtgccaga ccatgtagac acgcggcagg gcgacgctgt cctcctcctg catccgcatc
     9601 tcgcgctcca tgacgggctg acgggccgcg acccgtttga cctcggggcc gcgcgcgacg
     9661 gagccgaagt gcttctcgac gaggcggcgc gcctcggcgg gctggaagtc gccggcgatg
     9721 gtgaggctca cgttgttcgg cacgtagaag cggcggaaga agtctttcac gtcgtccatc
     9781 gaggcggctg tgagatcgtc gagtgagccg atggtgagcc agtggtacgg gtgctcgggc
     9841 gggtaagtgg cggcggcgat tttggcgccg acgaggccgt aagggcggtt gtcgtagttc
     9901 tgccgcttct cgttcttcac cacgtcgcgc tggttggcga gcttctcctc ggtcatggcg
     9961 tcgaggaggt tcgccatgcg gtcggcttcg aggtagaggg cggtctccaa aaagttcgag
    10021 gggacgacct cgtagtagtt ggtgcggtcg gggttggtcg agccgttgat attgccgccg
    10081 gcctcctgaa gcgggaagaa gtagtcgtag tcgaagttct tcgacccctg aaacatcatg
    10141 tgctcgaaga ggtgcgcgaa gccggtcttg ccgggcacct cgttcttcga gccgacgtga
    10201 taccagagat tgacggccac gatgggcgcc gaatgatcct cgtgcagcac gacgcgcagg
    10261 ccgttgggca gcgtgaactc attgaccttc aggtcgagcc gcgaggctcc cggcgcaacg
    10321 gccgcggccg gcttcggtct cttggacttc tgcgcgaggg ccgggagacc ggccgtcaac
    10381 agcacgacgg cgacgacggc ggccaacaat ctttgcagac gcatatcaac acactcctta
    10441 ccgcccgcgg gcggttagca agggcgaccc cgtgcgtgcc cgagacgagg gcgggcacgc
    10501 cttgcggggg ccgcgataag cttgtgaaat tcttagggga acgaaacgaa gttgatgagc
    10561 gcattctaac ctgcgagtga acccggtgca agattgcccg ccgccggcag ggttgttaga
    10621 gccgcgctgc ctcgcaaaca aaacaattaa cctgtctgcg ggataagggg acggcgcaat
    10681 cggcagacgt gaggggccgg gtgtgtgtgt cggtcagaga ggtttgaatg tgtgtggctc
    10741 cggccgcgtc gcccaagccg gcgcggccgg agcgggcgac gccgttaacg ggtcgccttg
    10801 tctgacttgc cgcagttggc gttcatccac ttctcgcagt cgcgttgact cgccggcccg
    10861 aagacctgtg tgtagatggc ggggtaaacg tcgccgtgcg ggatcacctt gcagtcttcg
    10921 tgcttcttcc cggcgcccgc ggtcagggcg atgacgatgt aggctctttg tctgtcgtcc
    10981 acgattgctc tcctttcgtt cggcggtcgt tgtggtgagg cccgcgctcg tcaggctcac
    11041 gcgagcagaa aagagatatg gtagcgcgcg ggcacgatat ctccgccgcc gagccggtgt
    11101 caacgccggc ccgctctcgt tgcttccgcc gacagccgct tgttaaaatc cgcagcgatc
    11161 attcacacaa ccgaacacgt gatgctggag acagattaca tcatcgtcgg cagcggcatc
    11221 gccggcctgc gcgcggccat cgagctggcg cgcgcgggcg cgcgcgtgac cgtgctgacg
    11281 aaggatcggc ccgaggagtc gaacacggag tacgctcagg gcggcatcgc cgtggcgctc
    11341 gccgaggacg atgaggcgtc gctgcacgag gacgacacgg ccgccgcggg cgcggggctg
    11401 tgcgacgagc gggcggtgag cgtgctcgtc gaggacggcc gcgccggggt gcgcgagttg
    11461 atcgggtggg gcgcggagtt cgaccgcgag ggcggccggc tcatgttcac gcaggaggcc
    11521 gcacactcgc gccggcgcat cctccacgcc ggcggcgacg ccacgggcca cgagatcgga
    11581 cgcgccctca tcgcccgcgc cggacacgag cgcaacctct cgttcgtgcc gcacgcggcg
    11641 accgagagtc tgatcgtgga agacggccga gtgggcggcg tgcgctacct cgacccgctc
    11701 gtgcgccgcg cgcgcgagat gcgcgcgcgc ggcgtcgtac tcgcctcggg cggcgctggc
    11761 cagctctttt tgcacacgac caaccctgaa gtcgcgaccg gcgacggcat ggcgatggcc
    11821 tacctcgccg gggcggaact ggccgacatg gagttcgtac agtttcaccc gacggcctta
    11881 aacgtcgccg gcgcgccgcg cttcctgctc tccgaggcgc tgcgcggcga gggcggggtg
    11941 ctccgaaacg ccgagggccg acgcttcatg ccgcgctacc acgagcgggc cgagcttgcc
    12001 ccgcgcgacg tggtcagccg ctccatcgtc gccgagatgg agcgcaccgc gacgcgcacc
    12061 gtctatctcg acatgacggg actcgaagcg cgcttcgttc gctcgcgctt ccccgtcatc
    12121 tactccacct gcctgcgctt cggtctcgac ctcacacgcg agccggtgcc cgtcagcccg
    12181 gcggcgcact acatcatggg cggcgtccgc acagacctcg acggccgcac cacgctcccc
    12241 ggcctctacg ccgccggcga ggtcgcctgc acgggcgtcc acggcgcgaa ccgtctggcc
    12301 tcgaactctc tgctcgaagg tctggtgttc ggcgcacgcg cgggccgtgc ggcggcgcga
    12361 gaattcgcgg acaagccggc gccgcgccgt aatcttacgg cgcggcgcga cgcggaagag
    12421 accgctaccg cggagcatca ttcggaagag caggggcgcg cgggcgcggc ggcgctggcc
    12481 gtgcgtaagc gcgtgcgtcg cctgatgtgg gagcgcgtcg gcatcctgcg ctcgcgcaac
    12541 tctctggaac gcgccctgcg cgagttcgag gagatcggcc gcgcaccctt gcgaccgggg
    12601 ccgcgcaact tcctcacggt ggccacgctc gtcgcgcgtt cggcattgtg gcgcgaggag
    12661 tcgcgcggcg cgcaccaccg cgtcgatttc ccagagcgcg acgatgcgcg ctggcgccgg
    12721 cactcgatca tccagaggga cggcggggtt tcgtcgagcg agacggtcga attcgccgcg
    12781 acggtggaga aattgtaggg gcaggcttgt ccctgccctt gcttacagaa caattaacat
    12841 ggatgggtag gatgatcagg ataagagatt taaaatttga aatctcaaat cttgaatctc
    12901 aaatcagcac ttgcttcatc ctgttaatcc tgtccatcca tgttaattgc ttcacagcac
    12961 gggccgcgga cttgcgctgc ccctacaaag aatcatcgtt gattaactca tggcccagta
    13021 cgaaaagctg cacgattggg acttgaagcc gcgcgaggcg attgagcttc agaaacgttt
    13081 acgcgagcgt gtggaggtcg ttccgctcaa gcgcaaggtc gagaccatcg cgggcgcgga
    13141 catctctttc aacaagttcg agccgacggt ctacgcgggc ctcgtcgtgc tgcgtctgcc
    13201 gacgctcgaa gtggtcgagg aggtcggggt cgtgagcgag acgaagtttc catacgtgcc
    13261 ggggctgctg tcgtttcgcg agacgccgtc gctgctggag gcgtgggaga aactgaaggt
    13321 cgagccggac gccgtgatgt tcgacgggca gggcctcgcg cacccgcgcc gcgtcggtat
    13381 cgcctcgcac gtcgggctga tgttgaagcg gccgacgctc ggctgcgcga agtcggtgct
    13441 ggtcgggaag ttcgaggagc cgggcgagga gcgcggcagt tggtcgccga tgacggacaa
    13501 gggcgagacg gttggggcgg ccgtgcggac gaagacgcgc gtgcagcccg tctacgtctc
    13561 gcccggccac ctcatcgacc tcgaaggcgc aatcgatctc gtgctccgca ctgacggcgg
    13621 ctatcgccag cccgagccga cgcgccgcgc acacctcctc gtcaacgcgc tgcgccgcgg
    13681 cgaacgcgcg cccacgtccg aagaggacgc cgggcgcaaa gacgccggcg acgcttcgca
    13741 gggcgcgttc cagttctaat cctccacatc ctttccgcct cctgcgacga gcgatttcaa
    13801 cttagaaaac ttcgggctgt acccgcgcca cgcgtaatcg ctca
//
LOCUS       DASVES010000120         7024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1032575, whole genome shotgun sequence.
ACCESSION   DASVES010000120 DASVES010000000
VERSION     DASVES010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032575"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1029)
                     /locus_tag="VF240_04385"
     CDS             complement(205..1029)
                     /locus_tag="VF240_04385"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7174505.1"
                     /translation="MSAAAANEEILVIETPERVPLHFALASTGNRFLACAFDHALQFL
                     LMAVVALGLLWAGSWLGFLYDRLVEAPKWMIAVLILAVFLVWSGYFALFEWAWNGQTP
                     GKRWLRLRVIREDGRPVTFWEAAARNVLRIFDMMPFPFYSVGLIAVFCSRRDQRVGDM
                     VAGTVVVREREAQAPSFEEVFNSPTSDLALRRSFKPFPFAADVSALAEPEVEVVESFL
                     RRRWDLPDATRAWMAWRVATPILHKLRPTFDPAAFTYEGFLEELLHRYRAAHRFRD"
     gene            complement(1035..2417)
                     /locus_tag="VF240_04390"
     CDS             complement(1035..2417)
                     /locus_tag="VF240_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX7174506.1"
                     /translation="MSEKKITRREFVAGATAGAALARTSSARVQSSGQSRRPNVLFIL
                     ADDLGWGDLSCYGRPDYRTPHLDALAREGTRFTEAYSASPVCTPTRCAFMTGQYPARH
                     PVGLEEPLHERSVVGTRVGLDPALPTIASLLRAANYETALVGKWHLGYLPKYGPTKSG
                     FDEFFGIMSGAADFFTHDDMTGERDFFENEQSVPPTRVGYMTELLTERAVEYVRRRRA
                     RPFYLSLHYTAPHWPWEGPNDAEAAKKRKRGYREFRGGGSLKTYAEMMRSLDDGIGRV
                     LRALRDAGLERSTLVVFTSDNGGEHFSYNWPFTGQKGSLREGGIRVPAIVRWPGVVAA
                     GRTTEQVAVTMDWTATILAATGTQADPQHRLDGIDLSHFIRTGGARAPVARTLFWCFG
                     GQSAARRGRLKYLNDGRAEYLFDLASDVRENGDLKNVRVREFEQLKAEFENWDRQVLP
                     RPPARPPLLP"
     gene            complement(2414..2872)
                     /locus_tag="VF240_04395"
     CDS             complement(2414..2872)
                     /locus_tag="VF240_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX7174507.1"
                     /translation="MSEDNNTLIRRWFEEVWNKGRSEAIDELFAADGVAHGLADAAGG
                     ELRGPADFKPFFQRFRESFPDIEIVVEDTISEGDRVAARCTVRARHQGDSLGFAATGQ
                     TVEFTGIAIVRVSQGQIVEAWNNFDFMSMYQQLGALRLHAPGTEGFIGDM"
     gene            complement(2956..4188)
                     /gene="tyrS"
                     /locus_tag="VF240_04400"
     CDS             complement(2956..4188)
                     /gene="tyrS"
                     /locus_tag="VF240_04400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7174508.1"
                     /translation="MNYRIPPMTIDEQLSFLRKGTVDVIREEDLRAKLERSARDGRPL
                     NVKLGADPTAPDIHLGHTVVIRKLRAFQELGHTVTFLIGDFTGLIGDPSGKSATRPQL
                     TREEINANAETYKKQIFKLLDPEKTVIDFNSRWMDALGSDGFVRLASHVTVKQILERD
                     DFAKRISEERPVALHELLYPLAQAYDSVALKADVELGGTDQKFNLLMARNLQREYGQE
                     PQAVLTMPLLEGTDGVQKMSKSLGNYIGIDEPPAEMFGKIMSISDDLMWRYYELLTSL
                     TIEQIQERRERASNGDVNPRDLKVDLAKRIITDFHSSDAGREAEEEFNRIFKRKEVPE
                     EIETRKIEAGSWPLPRLLVRTELAPSVAEARRLIEQGGVRIGGVRQTEVGVVVAVITG
                     EDVLLQVGKRRFLRVRGE"
     gene            complement(4260..4889)
                     /gene="purN"
                     /locus_tag="VF240_04405"
     CDS             complement(4260..4889)
                     /gene="purN"
                     /locus_tag="VF240_04405"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7174509.1"
                     /translation="MTDLEHEARPKRIAVLISGRGSNMTALADAVGAGRCGRAEIVLV
                     LSDRAGAAGLAVAAERALETHVVERRGRTREEHEREIIAALRARGVELVCLAGYMRLL
                     SPCFVEEFRASVLNIHPSLLPAFPGLDAQRQALAHGVRVSGCTVHFVDETLDGGPIIT
                     QRTVPVEDSDTEATLSARILAEEHRAYPEALALVVGGRYEIVGRRVVEK"
     gene            complement(4950..6278)
                     /locus_tag="VF240_04410"
     CDS             complement(4950..6278)
                     /locus_tag="VF240_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174510.1"
                     /translation="MMNRLTALACALAFAICAGTDARADVRIKQKVTVGGHTMESDVA
                     IKGQRQRTESEMAPGMKTVQIMQCDLRRMLQVSDATRKYTVTPLGGGDPTEASRPATP
                     APATSGVARRGGVVTYTTTMTDTGERKQMFGFTARRIKSSMRMEASPDACQKDGMNIE
                     TDGWYIDFDFQFSCPMDGSQAMTQIPARPDGCQDQVRFRSTGTAKLGYPVQTTTRMLD
                     MNGRELSSTSTEVTSLTRATLDAALFDVPAGYVETKNAQELYDTAAMMRAAQSARSND
                     DDNAAGRRDGPMNVPATSSPGAKRAGVIRVGVVALNNRTDKTVETEQLRRQLIAELTG
                     ATVEAVALDTSSPEEARQKECDFILSTDISALKQSAANKIGGMFGRAAGVGGAAERFE
                     AKVEYTLVPASGGAALVQSNASAKEEGGADASLSAALRKEAQAVLAKVRK"
     gene            complement(6530..>7024)
                     /locus_tag="VF240_04415"
     CDS             complement(6530..>7024)
                     /locus_tag="VF240_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7174511.1"
                     /translation="ALPSAGLHTNGYSLARKLFFERAGLQPDSHVERLGSTIGEALLA
                     PHLSYLRPLDGLLDTGAVKGLAHITGGGLLENIPRILPEDRGVEIRRGSWPVLPVFEL
                     MRELGNVPEREMLRTFNMGVGMVVVCAPADARAVRSHVESAGAACHEIGRVVEGERAV
                     TLTD"
BASE COUNT         1128 a         2212 c         2433 g         1251 t
ORIGIN      
        1 aagtcccttc tgtcgaaaat ctcgtcacac gctgaacccg tcattttagc cgacgccgaa
       61 ataggggtca atttaaggtg cggccggcag cgttgtcaga cagtaccgcg agcgggagcc
      121 gcgcgggtct cgcgtgcgcg cggcgggcgt cgcatcacag gcgtacccgc gaggcttccg
      181 ctcgcggtac tgttgtaaac cgccctaatc cctgaagcga tgcgcggcgc ggtagcggtg
      241 gaggagttct tcgaggaagc cttcgtaggt gaaggcggcg gggtcgaagg tgggacgcag
      301 tttgtggagg atgggcgtgg cgacgcgcca ggccatccac gcgcgcgtcg cgtcgggcaa
      361 atcccagcgg cggcggagga aggattcgac gacctccacc tccggctcgg cgagcgccga
      421 cacgtcggcc gcgaagggga aaggtttgaa agagcgtcgc agcgccagat cgctcgtcgg
      481 cgagttgaag acctcctcga acgagggggc ttgcgcttcg cgctcgcgca cgaccaccgt
      541 gcccgccacc atgtcgccca cgcgctggtc acgccggcta cagaagaccg cgatgaggcc
      601 gaccgagtag aaagggaacg gcatcatgtc gaagatgcgc aggacgttgc gggcggcggc
      661 ctcccagaag gtgacggggc ggccgtcctc gcggataaca cgcagacgca gccaacgctt
      721 gcccggcgtc tgcccgttcc aagcccactc gaagagggcg aagtagcccg accagacgag
      781 gaagacggcg aggatgagga cggcgatcat ccacttcggc gcttcgacca gacggtcgta
      841 gaggaagccg agccaactgc cggcccagag cagaccgagg gcgacgacgg ccatgagcag
      901 gaactggagc gcgtgatcga aggcgcaggc gaggaagcga ttgcccgtcg aggccagcgc
      961 gaagtgcagc ggcacgcgct caggcgtctc aatgaccaga atctcttcgt tggcggcggc
     1021 tgctgacatt tctctcaggg taagagcgga gggcgagcag gcggtctggg gagcacttgt
     1081 ctgtcccagt tctcgaactc cgccttcaac tgctcgaact cgcgcacgcg cacattcttc
     1141 aagtcgccgt tctccctcac gtcagacgcg aggtcgaaaa gatactccgc gcggccgtcg
     1201 ttgaggtact tcagtcggcc acggcgcgcg gcgctctgcc cgccgaagca ccagaagagt
     1261 gtacgcgcga ccggcgcgcg cgcgccgccg gtgcggatga agtgggagag gtctattccg
     1321 tcgagccgat gctgcgggtc tgcttgcgtc cccgtagcgg cgaggatcgt cgccgtccag
     1381 tccatcgtga cggcgacctg ctcggtggtg cgccccgcgg cgacgacgcc gggccagcgc
     1441 acgatggccg ggacgcgtat gccgccctcg cgcaggctcc ccttctgacc cgtgaagggc
     1501 cagttgtagg agaagtgctc gccgccgttg tcgctcgtga agacgacgag cgtcgagcgc
     1561 tcaagccccg cgtcgcgtag cgcgcggagc acgcgaccga tgccgtcgtc gaggctacgc
     1621 atcatctcgg cgtaggtttt gagtgagccg ccgccgcgaa actcgcggta gccgcgcttc
     1681 cgcttcttcg ccgcctccgc gtcattaggc ccctcccagg gccagtgcgg cgcggtgtag
     1741 tggaggctca gatagaacgg gcgggcgcgc ctgcgcctga cgtactcgac cgcgcgctcg
     1801 gtcagcagct ccgtcatgta gccgacgcgc gtgggcggga cgctttgttc gttctcgaag
     1861 aagtcgcgct cgcccgtcat gtcgtcgtgc gtgaagaagt cggccgcgcc gctcatgatg
     1921 ccgaagaact cgtcgaagcc gctctttgtg gggccgtact tcgggagata gccgaggtgc
     1981 cacttgccga cgagcgccgt ctcgtaattc gcggcgcgca ggagcgaggc gatagtcggc
     2041 agcgccgggt caagaccgac gcgtgtgccc acgaccgaac gctcgtgcag cggctcctcc
     2101 agcccgacgg ggtggcgcgc ggggtactgc cccgtcatga aggcgcagcg cgtcggcgtg
     2161 cagacgggcg aggccgagta ggcttccgta aagcgcgtgc cctcgcgggc gagcgcgtcg
     2221 aggtgcggtg tgcggtagtc ggggcgtccg tagcaactga ggtcgcccca tccgaggtcg
     2281 tcggcgagga tgaagaggac gtttggccga cgcgactggc cggacgactg gacgcgcgcc
     2341 gaagacgtgc gcgcgagcgc cgcgccggcc gtggccccgg cgacgaactc ccggcgggtg
     2401 atcttttttt cgctcacatg tctcctatga agccttcggt tccgggcgcg tggagccgga
     2461 gcgcgccgag ttgctggtac atcgacatga agtcgaagtt gttccacgcc tcgacgatct
     2521 ggccttgcga gacgcggacg atggcgatgc cggtgaactc gaccgtctgc cccgtcgccg
     2581 cgaacccgag cgagtcgccc tgatgacggg cgcgcaccgt gcagcgcgcc gcgacacggt
     2641 cgccctcgga gatcgtgtcc tcgaccacga tctcgatgtc ggggaacgac tcgcggaacc
     2701 tttgaaagaa cggcttgaag tcggcggggc cgcgcagctc gccgccggcc gcgtccgcca
     2761 gcccgtgcgc gacgccgtcc gcggcgaaca gctcgtcgat ggcctcggag cgccccttgt
     2821 tccagacctc ctcgaaccag cggcggatga gtgtattgtt atcctctgac atctcggtcg
     2881 ctcctttcgt gagggacttc gttccacgcg cgggatgaaa gtggcagccc ggcgcgtcct
     2941 tcgttgcgtg cgcctctact cgccgcgcac gcgcaggaag cggcgcttgc cgacttggag
     3001 gagcacgtct tcgcccgtga tgactgccac caccactccc acctcggttt gacgcacgcc
     3061 cccgattcgc accccgccct gttctattag tcgtcgcgcc tcggcgactg acggggccaa
     3121 ctccgtgcgg acgagcaggc gcggcagcgg ccatgagcct gcttctattt tgcgtgtttc
     3181 aatctcctca ggcacttcct tacgcttgaa gatgcgattg aactcctcct cggcctctcg
     3241 cccagcatcc gatgagtgga agtcagtgat gatgcgcttg gcgagatcaa ccttaaggtc
     3301 gcgaggatta acgtctccat ttgaagcacg ttcgcgacgc tcttggattt gttcaatagt
     3361 caaacttgtc agcagttcat agtagcgcca catgaggtcg tcgctgatgg acatgatctt
     3421 gccgaacatc tctgcgggcg gctcgtcgat gccgatgtag ttgcccagcg acttcgacat
     3481 cttctgcacg ccgtcggtgc cttccagaag cggcatggtg aggacggcct gcggctcctg
     3541 accgtactcg cgctggaggt tgcgggccat cagcaggttg aacttctgat cggtgccgcc
     3601 gagttccacg tccgccttca gcgcgaccga gtcgtaggct tgcgcgagcg ggtagaggag
     3661 ttcgtggagg gcgacggggc gctcctcact gatgcgcttg gcgaagtcgt cgcgctcaag
     3721 gatttgcttg acggtgacgt gcgaggcgag acggacgaag ccgtcggagc cgagcgcgtc
     3781 catccagcgg gagttgaaat cgatgacggt cttttccggg tcgagcagct tgaagatttg
     3841 ttttttgtag gtctcggcgt tcgcattaat ttcctcgcgc gtgagctgcg ggcgcgtggc
     3901 cgatttgccc gaggggtcgc cgatgaggcc ggtgaagtcg ccgatgagga acgtgacggt
     3961 gtggccgagc tcctgaaagg cgcgcagctt gcggatgacg accgtgtgac cgaggtggat
     4021 gtcgggcgcg gtcgggtcgg ccccgagctt gacgttgagc ggtcttccgt cgcgcgccga
     4081 cctttccagc ttcgcgcgca agtcctcctc gcggataaca tccaccgtgc ctttacgcag
     4141 gaaggaaagc tgctcgtcaa tcgtcatggg cggaattcta tagttcaaag ttcaaagttc
     4201 aaagttccag attcaaagct tcagagaaac catttacgat ttcgacttgg aactttgaac
     4261 tacttctcga cgacgcggcg gccgacgatt tcgtagcggc cgccgacgac gagggcgagg
     4321 gcctcggggt aggcgcggtg ttcctcggcg aggatgcggg ccgagagcgt ggcctcggtg
     4381 tcggagtctt cgacggggac agtgcgctgc gtgatgatgg ggccgccgtc gagcgtctcg
     4441 tcaacgaagt ggacggtgca gccggagacg cggacgccgt gggcgagggc ctgccgctgc
     4501 gcgtcgagac cggggaaggc cgggagcagg gaggggtgga tgttgagaac ggaagcgcga
     4561 aactcttcga cgaagcaggg cgacagcagc ctcatgtagc cggcgaggca gacgagttcg
     4621 acgccgcgcg cccgaagggc cgcgatgatc tcgcgctcgt gctcctcgcg cgtgcggccc
     4681 cggcgctcga cgacgtgcgt ctccagagcg cgctcggccg cgacggcgag gcccgccgcc
     4741 ccggccctgt cgcttaagac gagcacgatc tcggcgcgcc cgcaacgccc ggcgccgacg
     4801 gcgtcggcga gcgcggtcat gttcgagccg cgcccggaga tgaggacggc gatgcgcttc
     4861 gggcgcgcct cgtgttcaag gtcggtcatc gacttatgca gcggcgacgg agacggcgcc
     4921 gctgctgtta aagtgttgat gcggcgtcct cacttgcgaa ctttcgcgag gacggcctgc
     4981 gcctctttcc tgagcgcggc cgaaagggac gcgtcagcgc cgccttcttc cttggccgag
     5041 gcgttcgact gcacgagggc cgcgccgccc gaagcgggca cgagcgtgta ttcgaccttc
     5101 gcctcgaacc gctccgccgc tccgccgacg ccggccgcgc gcccgaacat gccgccgatc
     5161 ttgttcgccg ccgactgctt gagggcggag atgtcggtcg agaggatgaa gtcgcactcc
     5221 ttctgccgcg cctcctccgg cgagctggtg tcgagcgcga cggcttcgac ggtggcgccg
     5281 gtcaactccg cgatgagttg ccggcgtagt tgttcggtct cgaccgtttt gtccgtccgg
     5341 ttgttcagcg cgacgacgcc cacgcgaatc acgcccgcgc gcttcgcccc cggcgacgaa
     5401 gtcgctggca cgttcatcgg cccgtcgcga cgacctgcgg cgttgtcgtc gtcgttgctg
     5461 cgcgccgact gcgcggcccg catcatcgcc gccgtgtcgt aaagctcctg cgcgtttttg
     5521 gtctcgacgt agccggcggg cacgtcgaag agcgccgcgt cgagcgtggc gcgcgtgagg
     5581 ctcgtgacct cggtgctcgt cgaggacaat tcgcggccgt tcatgtcgag catccgcgtg
     5641 gtggtctgta cggggtagcc gagtttggcc gtgcccgtgc tgcggaagcg cacctgatcc
     5701 tggcagccgt cggggcgcgc aggtatctgc gtcatcgcct gcgagccgtc catcgggcaa
     5761 ctgaactgga agtcgaagtc gatgtaccag ccatcggtct cgatgttcat gccgtccttc
     5821 tggcaggcgt cgggtgaagc ctccatccgc atggacgact tgatgcggcg cgcggtgaag
     5881 ccgaacatct gtttgcgctc gccggtgtcg gtcatcgtcg tcgtgtaggt gacgacgccg
     5941 ccgcggcggg cgacgcctga agtggcggga gccggcgtcg cggggcggga cgcctcggtc
     6001 gggtcgccgc cgccgagcgg tgtgacggtg tacttccgcg tcgcgtcgct cacctgaagc
     6061 atgcgcctga ggtcgcactg catgatctgg acggtcttca tgccgggcgc catctcggac
     6121 tcggtgcgct ggcgctggcc cttgatcgcc acgtcgctct ccatcgtgtg cccgccgacg
     6181 gtcaccttct gcttgatacg cacgtcggcg cgcgcatccg tcccggcgca gatcgcgaag
     6241 gcgagggcgc acgcgagcgc ggtcagtcgg ttcatcatgt cgttactcct tcgtgttcgt
     6301 ggcagaaatt ttatggaagc ggcctcataa atgtggggtc gcaggataat gagccacgaa
     6361 gtcacactaa ctgacgcgag aactgacgcg aaaaaggctc aactcattac gggtgtaact
     6421 cctgttcgtg tcgctttgtg tgacttcgtg gctcatcgtc ccttttaata tttatgagac
     6481 gggttgtcat taagacaggc aagattagcg acggcgcgcg gcgcgacttt caatccgtca
     6541 gcgtgacggc gcgctcgccc tcgacgacgc gcccgatctc gtggcacgcc gcgccggccg
     6601 actcgacgtg cgagcggacg gcccgcgcgt ccgcaggcgc gcagacgacg accatgccga
     6661 cgcccatgtt gaaggtgcgt agcatctcgc gctcgggcac gttgcccaac tcgcgcatca
     6721 gctcgaagac gggcagcacc ggccacgagc cgcggcggat ttccacgccg cggtcttcgg
     6781 gcaggatgcg cgggatgttc tcaaggagtc cgccgcccgt gatgtgcgcc agtcccttga
     6841 cggcgcccgt gtcgagcagg ccatcgagcg ggcgcaggta actgaggtgc ggcgcgagca
     6901 gcgcctcgcc gatggtcgag ccgaggcgtt cgacgtggga gtcgggttgc aggcccgccc
     6961 gttcgaagaa gagtttgcgg gcgagcgagt agccgttggt gtggagaccg gccgagggga
     7021 gcgc
//
LOCUS       DASVES010000121         8973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_810269, whole genome shotgun sequence.
ACCESSION   DASVES010000121 DASVES010000000
VERSION     DASVES010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810269"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..675)
                     /locus_tag="VF240_04420"
     CDS             complement(265..675)
                     /locus_tag="VF240_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7174512.1"
                     /translation="MSVTGLELLFLEVNSLEESLAFYAGALGFELLAHAPEAEPPLAT
                     LRAGRLRLTLAQVPITMVRRGRGVHLFLTVSDVDEFHERLRALSFDLAPPTDEGWGGR
                     FITLEDPDRYRIFLVAWNDRPNEKAGDGPMASLN"
     gene            complement(680..1249)
                     /locus_tag="VF240_04425"
     CDS             complement(680..1249)
                     /locus_tag="VF240_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7174513.1"
                     /translation="MTRDEDADDYYTTLGADEAASHDEIERLYKGLAREHHPDRGGDE
                     ERMKSINEAYRVLGNEASREVYDAERGHTRSPDTGYYYTPVYSSPPSQADAIGGRILG
                     AWLCMLAGLVLLFLVRFHYVRFLWPLALLAVAIVLMGVVMAHGALVFARKHGEPRALF
                     GRFAWAQELAFWSFMCAGAYGVYLVLTAV"
     gene            1432..1788
                     /locus_tag="VF240_04430"
     CDS             1432..1788
                     /locus_tag="VF240_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174514.1"
                     /translation="MSQSFAPGDDLIFQLESGFGLMRVLGVEGEGPATVWHVLVYEDF
                     YPDVEAAEAALAGAEPPGVGWPHLPLTDRALERTPAARLGNRPVADAELAPINAWRER
                     GGEAFDRSVLQMIGVR"
     gene            complement(1799..5608)
                     /locus_tag="VF240_04435"
     CDS             complement(1799..5608)
                     /locus_tag="VF240_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7174515.1"
                     /translation="MSGLNRLKLLVLILFVLTGAALFTNNAVNRRVHAFSAGPPAGYT
                     RAPGEEPEACAECHLADGLGTGAGTISIDAPQTYVPGQVYQITVTHTNSDLTRVRWGF
                     QLTALDTSEERAGSLEPLDGYTQVLSGQGPFPDRQYIEHTQPGTFFGQTGGASWAFRW
                     TAPATDVGPVTFYAAGNHANGDQNSSGDNIYFTFVSATPAAPSPDFSISVTPSTRIVT
                     PGESVTYDVTVTPFAGFTGTVNLSLTTPPPNTTSSFNPPSLNISDASAQTSVLTLTAL
                     GGVTSPVGSSRFNVNATSGALQHSAQATLINAAPESTELAVTMTDSPDPVPLRANLSY
                     RITVTNNGPGAATGVTLTDTLPASASFVSATPTQGSCSGTTTVTCALGDLAAGASAVV
                     NIVVQPQAIGHINNTASVSGAQGDPYFANNTVSISTTVEQQGSTPLMLVPNLSVRTIV
                     SGLDQPTGLAFLAANDFLVLEKATGRVRRVVNGGVQATVLDLSVNSASERGLLGVALH
                     PNFAANKFVYLYWTESPTGDDTTDTAAVFLLGNRVDRYVWNGSTLIFDRNLIRLRAHQ
                     EDEGQPLRGNHNGGRLAFGPDGKLYIMIGDVGRRGFLQNVTTAVGPAGKDDPWGGPEP
                     DDAHLTGVVLRLNDDGTTPTDNPFFNVATGLTTPTAADNVKKVFAYGVRNGFGMAFDP
                     LGGKLWTQEHGDDAFDEINRVEAGSNGGWIQLMGPLSRVSEYKSIELARAGGLQQLRW
                     PPSLIADTPQQALQRLYQLPGSTYTDPQLSWKYAVAPSPLGFVAGQGLGPQYAGDMFL
                     GASRTTLLGGYLFRLKLSADRLRLATTDARLADGVADNADKFDLTESESLVAGRDFGI
                     TTDIQTGPDGNLYVVSLSNGAVYEIFARSELFVANLTGAQEVPPNNSAATGTATLTLA
                     TDGATARITLDFDGLSSQQTDAHVHGPAAPGASAGILFDLPDGDFSDFQITLTPQQVA
                     ELRAGRLYVNVHSQNFPGGEIRGQFGGPTSAPTSANPIDDPAFFVRQHYYDFLNRAPD
                     ASGLAFWKNQITECETRPEAERQGCREVRRINVSAAFFLSIEFQETGYYVLRTQRAAF
                     GRRSDTAPLRLAYAEFVRDARQVGEGVVVGQAGVEQLLEQNKQAYAEAVVGAAGFAAR
                     FPLTLGAAEYVDALFASAGVAPTPAERQEAIDAFGAGASAGRVSALRKAADSGSLRRA
                     ELNPAFVLLEYYGYLRRNPTDAPDTDDSGYQFWLAKLNSFGGNYIAAEMVKAFITSSE
                     YRARFGN"
     gene            complement(5703..7106)
                     /locus_tag="VF240_04440"
     CDS             complement(5703..7106)
                     /locus_tag="VF240_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7174516.1"
                     /translation="MPTSRRDFLRRTGCLLGGAAIASTLDGFKLLSAYAQDSAADDYR
                     ALVCVFLNGGNDGNNMVVPFDDARYNQYNAARGAAGLAIPKASLLAVNPASGNQYGLH
                     PNMPELQTLFGQGKLAVVCNTGPLVEPLTRATYQSGAGKRPLQLFSHSDQVNLWQTSI
                     ANNASQTGWGGRTADRTAPLNGAATFPQVVSIAGISTFVIGANARPLAISDSNTALSS
                     VLPLNNAPNATTAETNARRAAFDRLRALEGGVVLVKASSDTTSSALQTRDALISAPAR
                     EFPAFIQTDSLARQLEQVARLIALRDTFGVKRQIFFCSLGGFDTHANQRNAGVPNNQD
                     SLLQQVSRALKAFYDATVELGVESSVTTFTLSDFGRTLQPAGAGAGVGSDHGWGNHQF
                     VMGGAVRGNDFYGSFPTLALGGPDDTDTRGRWIPTTSVEQYAATLAAWYGLSASDLPA
                     VFPLIGRFSTPTLGFML"
     gene            complement(7112..>8973)
                     /locus_tag="VF240_04445"
     CDS             complement(7112..>8973)
                     /locus_tag="VF240_04445"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX7174517.1"
                     /translation="RDVGQGWLFAIVLRLNDEMADDLGDVLVRINLHGVSSNRVRMAL
                     GQAGGGPPVDAVAEVISPAPAATPTPMPALQPKAYGPNETADADAVRLLEQASWGPTE
                     GEVARVKSIGFRQYVNEQLGAPATNYVDMLFPNDQSAQQCPDSLGPALQGDCFRDNYS
                     MYPLQKNFFSNAMYGQSQLRHRVGFALHQVLVVSGRDIPRPSWMTPYLQAIDRNATGS
                     YRQMLRDVTLTPAMGDYLDMNRSTRTSPNENFAREVLQLFSTGVDELNLDGTPRLDAQ
                     GARIPVYTQNTVNEFTRVFTGWTFGPAIAAGVTNYRDPMVPRANQAANHDQGAKTLLG
                     GAMLPAGQTAQADLDAALDNIFNHPNVAPFIGKQLIQHLVTSNPSPAYVERVARVFNN
                     DCDALYPDGCTGARGQMGAVVRSVLLDPEARGDVKTDPAYGRLREPVQYMSNVLRALD
                     AKSFDRTQQSDGVLTARASGGNDYAALMDQPLFLPPTVFSYYPPDYEVPGTGLVGPTF
                     NILSTSTALRRANFINQIVYTGYAATTGANTNSPRGTSLDLAPLEAIAADPQALASRL
                     DALMLHGTMSASMRAEIVAAVAVVPADSAANLRRRAQMAVYLVATSSQYQVQR"
BASE COUNT         1431 a         2678 c         3315 g         1549 t
ORIGIN      
        1 ggccgggccg cgtgccgaac tgggtttgtc ggatcgggag cttgcggaag ttattttaca
       61 tcattagcaa cttgagcgta atgcacgcgg gattcaccgc agaggcgcag aggacgctga
      121 aggagcgcag agggttgagc ctttcactcg tcgttgagtg tatgcgtcaa gcctctgcgt
      181 agtctctgcg tcctctgcgc ctctgcggtg agtgtgctta ctcgaacact caactgtgaa
      241 gctacaacac ttctgaggca gactttagtt caaacttgcc atcggcccat cgcccgcctt
      301 ctcgttcggt ctgtcattcc acgcgacgag gaagatgcga tagcggtcgg ggtcttccag
      361 cgtgatgaag cgtccgcccc agccctcgtc ggtcggcggc gcgaggtcga agctcagggc
      421 gcgcaggcgt tcgtggaatt cgtcaacgtc agagaccgtg aggaagaggt ggacgccgcg
      481 cccgcggcgc accatcgtga tgggcacctg cgcgagcgtc aggcgcaggc ggcccgcgcg
      541 cagcgtcgcc agcggcggct cggcctcggg cgcgtgcgcc agcagctcga acccgagcgc
      601 ccccgcgtag aacgccagcg attcttcgag gctgttgacc tccagaaaga gcagttcgag
      661 tcccgtcacg ctcatcgctt cacaccgccg tcaaaacgag atagacgccg tatgccccgg
      721 cgcacatgaa cgaccagaag gccaactcct gcgcccacgc gaagcggccg aagagcgctc
      781 gcggctcgcc atgtttacgt gcgaagacga gtgcgccgtg cgccatgacc actcccatca
      841 ggactatcgc aaccgcgagg agcgcgagcg gccacaggaa cctgacgtaa tgaaatctca
      901 ccagaaagag gagcaccaat ccggccagca tgcagagcca cgcgccgagg atgcgtccgc
      961 cgatggcgtc ggcctgcgag ggcggcgagg agtatacggg ggtgtaataa tatcctgtgt
     1021 cgggcgagcg cgtgtgaccg cgctcagcgt cgtagacctc gcgggaggct tcgttgccga
     1081 ggacgcgata cgcctcgttg atcgacttca tgcgctcctc gtcgccgccg cggtcgggat
     1141 gatgctcgcg cgcgaggccc ttgtagagac gctcgatctc gtcgtgcgag gccgcctcgt
     1201 cggctcccag cgtcgtgtag tagtcgtcag cgtcttcgtc tcgcgtcatt acagttttcg
     1261 ctggcccgct gtttcgagtg aagacaatac taaacgcaga cggagaaccg cgtccacacg
     1321 gccgccgccc cgagtgaacg cgcgacggac gcgcgcgtcc gcgtgacgcc ggcctcttag
     1381 attttttatg atagggctga acgaggatga aggtggcggg gtgccgaact tatgtcgcag
     1441 agctttgcgc cgggtgacga tttaatattt caactggaga gcggcttcgg cctgatgcgt
     1501 gtgctcgggg ttgagggcga ggggccggcg accgtgtggc acgtcctcgt ctacgaagac
     1561 ttctacccgg acgtggaagc ggctgaggcg gcgctcgcgg gcgcggagcc tccgggcgtc
     1621 ggctggcccc acctgccgct caccgaccgc gcgcttgaaa ggacgccggc cgcgcgcctc
     1681 ggcaaccggc ccgtggcgga tgccgagcta gcgcccatca acgcatggcg tgagcgcggc
     1741 ggcgaagcgt tcgaccgttc cgtgttgcag atgatcgggg ttcgctgaag cgcccggttc
     1801 aattcccgaa gcgcgcgcga tattcgctgc tcgtgatgaa ggctttgacc atctcggccg
     1861 cgatgtagtt gccgccgaag ctgttgagct tcgcgagcca gaactggtag ccggagtcgt
     1921 ccgtgtcggg cgcgtcggtc gggttccgac gcaggtagcc gtagtattcg agcaggacga
     1981 aggccgggtt gagttcggct cgccttaagg agccggagtc ggccgccttg cgaagcgccg
     2041 acacgcgtcc cgcggaagcg cccgcgccga aggcgtcgat ggcctcctgt cgctcggccg
     2101 gcgtgggcgc gacgcccgcc gaggcaaaga gcgcgtccac gtactcggcc gcgccgagcg
     2161 tcaaggggaa gcgcgccgcg aaacccgcgg ctccgaccac ggcttcggcg taagcctgtt
     2221 tgttctgctc cagcagttgc tcgacgcctg cctgcccgac gacgacgccc tcacccacct
     2281 gccgcgcgtc gcggacgaac tccgcgtaag ccaagcgcaa cggggccgtg tccgacctcc
     2341 ggccgaaggc cgcgcgctgc gtgcggagga cgtagtagcc ggtctcctga aactcaatcg
     2401 acaggaagaa cgccgccgag acgttgatac ggcgcacctc tcgacacccc tgccgctcgg
     2461 cctcggggcg cgtctcgcat tccgtgatct ggttcttcca gaacgcgagg cccgacgcgt
     2521 cgggcgctcg gttgaggaag tcgtagtagt gctgtcggac gaagaacgcg gggtcgtcaa
     2581 tagggttcgc gctcgtgggg gccgatgtgg ggccgccgaa ctgcccgcgt atctcgccgc
     2641 cggggaagtt ctggctgtgg acgttgacgt agagtcggcc cgcgcgcagc tccgcgacct
     2701 gctgcggcgt gagtgtgatt tgaaaatcgg aaaagtcgcc gtcgggcaga tcgaataaga
     2761 tgccggcgct cgcgccgggc gcggcggggc cgtggacgtg cgcgtccgtc tgctggctcg
     2821 atagcccatc gaagtcgagt gtgatgcggg cggtcgcgcc gtccgtggcg agcgtgagcg
     2881 tggccgtgcc cgtcgcggcg gagttgttgg gcgggacttc ctgcgcgccc gtgaggttgg
     2941 cgacgaacaa ttcggagcgc gcgaagattt cgtagaccgc gccgttcgac aggctgacga
     3001 cgtagaggtt tccgtcaggg ccggtctgga tgtccgtggt gatgccgaag tcgcggcccg
     3061 cgacgaggct ttcgctctca gtgaggtcga acttgtcggc gttgtcggcc acgccgtcgg
     3121 caagacgcgc gtcggtcgtg gcgagcctca gccggtcggc gctgagcttg agccggaaga
     3181 ggtagccgcc gagcagggtc gtgcgcgaag cgccgaggaa catgtcgccg gcgtactgcg
     3241 gcccgagtcc ctgccccgcg acgaagccga gaggcgaggg cgcgacggcg tacttccaac
     3301 tgagctgggg gtcggtgtac gtggagccgg ggagttgata gagacgctgg agcgcctgct
     3361 ggggcgtgtc ggcgatgagc gagggcggcc agcgaagttg ttgcagaccg ccggcgcgcg
     3421 caagctcgat tgacttgtac tcactcacac gcgagagcgg ccccatgagc tgaatccaac
     3481 cgccgttcga gccggcctcg acgcggttga tctcgtcgaa ggcgtcgtcg ccatgctcct
     3541 gtgtccagag cttgccgccg agtgggtcga aggccatgcc gaagccgttg cggacgccgt
     3601 aggcgaagac ctttttgaca ttgtcagcgg cagtgggagt ggtgaggccc gtggcgacgt
     3661 taaagaacgg attgtccgtg ggcgtcgtgc cgtcgtcgtt gagacgaagg acgacgccgg
     3721 tcaggtgtgc gtcgtcgggt tcggggccgc cccacgggtc gtccttgccg gcggggccga
     3781 cggccgtcgt cacgttttgc aggaagccgc gacggcccac gtcgccgatc atgatgtaga
     3841 gcttgccgtc ggggccgaag gcgaggcgtc cgccgttgtg gttgccgcgc aggggctggc
     3901 cttcgtcctc ctgatgcgcg cgcaggcgga tgaggttacg gtcgaagata agcgtcgagc
     3961 cgttccagac gtagcggtca acgcggttgc cgagcaggaa gacggcggcc gtgtcggtcg
     4021 tgtcgtcgcc agtcgggctt tccgtccagt agaggtagac aaatttgttc gcagcgaaat
     4081 tcgggtggag ggcgacgccg agcaaaccgc gctcggaggc gctgttgacg ctcagatcga
     4141 gcaccgtggc ttgaacgccg ccgttcacga cacgccgcac gcggcccgtg gccttctcca
     4201 gaacgagaaa atcattcgcg gcgaggaagg ccagccccgt cggctggtcg agaccggaga
     4261 caattgtccg caccgagaga ttagggacga gcatcagcgg ggtgctgccc tgctgctcga
     4321 ccgtggtcga aatgctgacc gtgttattcg cgaagtaagg atcgccctgc gcgccgctca
     4381 cgcttgccgt gttgttgatg tggccgatgg cctgcggctg cacgacgatg ttgacgacgg
     4441 cgctcgcacc ggcggcgagg tcgccgagcg cgcacgtgac ggtcgtcgtg cccgagcacg
     4501 agccttgcgt cggcgtcgcg gagacgaagc tcgcgcttgc gggaagcgtg tccgtcagcg
     4561 taacgcccgt tgccgcgccg gggccgttat tcgtgacggt gatgcggtaa ctaagattgg
     4621 cgcgcaacgg gacggggtcg ggcgagtccg tcatcgtgac ggcgagttcg gtcgattcgg
     4681 gcgcggcatt gatgagcgtc gcctgcgcgg agtgttgaag cgcgccgctc gtcgcgttga
     4741 cgttgaaccg gctcgacccg acaggagacg tgacgccgcc cagcgccgtc aatgtcagca
     4801 cggacgtttg cgcggacgca tcgctaatgt tgagtgaagg cggattgaag ctcgatgtcg
     4861 tattcggggg cggcgtggtg agactcaggt tgactgtgcc cgtgaagccc gcgaaaggcg
     4921 tgacggtgac atcgtaggtc acgctctcgc ccggcgtgac gatgcgcgtg gacggggtga
     4981 cggagattga gaagtcgggc gacggggcgg cgggggtggc cgagacgaag gtgaagtaga
     5041 tgttatcgcc ggaggagttc tggtcgccgt tggcgtggtt gccggcggcg tagaaggtca
     5101 cggggccaac gtcggtcgcg ggggccgtcc agcggaacgc ccagctcgcg ccgccagtct
     5161 gtccgaagaa ggtgccgggc tgcgtgtgct cgatgtactg gcggtcgggg aacggcccct
     5221 gaccgctcag gacttgcgtg tatccgtcga gcggctcaag gctcccggcc cgctcctcgc
     5281 tcgtgtcgag cgcggtgagc tggaagcccc agcgcacgcg cgtcaggtcg ctgttcgtgt
     5341 gcgtgacggt aatttgatag acctgtccgg gcacgtatgt ctgcggcgcg tcgatgctga
     5401 ttgtgcccgc gcccgtgccg aggccatccg ccagatggca ctcggcgcaa gcctcgggtt
     5461 cttcacccgg cgcgcgcgtg tagccggcgg gcggccccgc ggagaaggca tgcacacggc
     5521 ggttcaccgc gttgtttgtg aagagggccg cgcccgtcag gacgaaaagg atgaggacga
     5581 gaagcttcag ccggttcaga ccagacatgc tttggaaacc ctttaggagt caggagtcag
     5641 gagtcagaat gaagaaggca gtgcttctgt ttttattctg actcctgact cctgactcct
     5701 tatcacagca tgaatccgag ggtcggcgtc gagaagcggc cgattaaggg gaagactgcc
     5761 gggaggtcgg aggccgacag accgtaccac gcggcgagcg tggccgcgta ctgttcgacg
     5821 gaggtcgtcg gtatccagcg gcctcgggtg tcggtgtcgt cggggccgcc gagggcgagc
     5881 gtggggaagg agccatagaa gtcgttgccg cgcaccgcgc cgcccatgac gaactggtga
     5941 ttgccccaac cgtggtcgga gccgacgccc gcgcccgcgc ccgccggctg gagcgtgcgc
     6001 ccgaaatcag acagggtgaa ggtggtgacg ctcgactcca cgccgagttc caccgtcgcg
     6061 tcgtagaagg ctttcagggc gcggctgacc tgctggagca gcgaatcctg attgttgggg
     6121 acgcccgcgt tgcgctggtt ggcgtgcgtg tcgaagccgc cgagcgagca gaagaaaatc
     6181 tgtcgcttga cgccgaaggt gtcgcgcagg gcgatgagcc gggcgacctg ctcaagctgc
     6241 cgcgccagcg agtcggtctg gatgaaggcc ggaaactcgc gcgccggggc gctgatgagc
     6301 gcgtcgcgcg tctgcaaggc gcttgaggtc gtgtcgctcg aagccttgac gagcacgacg
     6361 ccgccttcga gcgcgcgcag gcggtcgaag gcggcgcgac gcgcgttcgt ctcggcggtg
     6421 gtggcgttcg gcgcgttgtt gagcgggagc acagagctga gggccgtgtt cgagtccgag
     6481 atggcgaggg ggcgcgcgtt cgcgccgatg acgaaggtgc tgatgccggc gatggagacg
     6541 acctgcggga acgtggccgc gccgttgagt ggtgcggtgc ggtcggcggt gcgcccgccc
     6601 cagccggtct ggctggcgtt gttagcgatt gaggtctgcc agaggttcac ctgatcggag
     6661 tgggagaaga gttgcagcgg gcgcttcccc gccccgctct ggtaggtcgc gcgcgtcagc
     6721 ggctcgacga gcggccccgt gttgcagacg acggcgagct tgccctgacc gaacaaggtc
     6781 tgtagctccg gcatgttcgg gtggagtccg tactggttgc cgctcgcggg gttgaccgcg
     6841 agcaggctag ccttcgggat ggcgaggccg gcggcgccgc gcgcggcgtt gtactgattg
     6901 tagcgcgcgt cgtcgaaggg gacgaccatg ttgttgccgt cgttgccgcc gttgaggaag
     6961 acgcagacga gcgcgcggta atcgtcggcc gcggaatcct gcgcgtaggc gctgaggagc
     7021 ttgaagccgt cgagggtcga ggcgatggcg gccccgccga ggaggcagcc cgtgcggcgc
     7081 aggaagtctc tgcgtgaagt cggcatgaag tttacctttg cacctgatat tgcgacgagg
     7141 tggcgaccag atagacggcc atctgcgcgc gccggcgcag gttcgcggcc gagtcggccg
     7201 ggacgaccgc gacggccgcg acgatttcgg cgcgcatcga cgccgacatc gtgccgtgaa
     7261 gcatgagcgc gtcgagacgg ctggcgagcg cctgcgggtc agcggctatc gcttcgagcg
     7321 gcgcgaggtc gagcgacgtg ccgcgcgggg aattggtgtt ggcgccggtt gtggccgcgt
     7381 agccggtata gacgatctgg ttgatgaagt tcgcgcggcg gagcgccgtc gaggtcgaga
     7441 ggatgttgaa ggtggggccg acgaggccgg tgcccggcac ctcgtaatcc ggcgggtagt
     7501 agctgaagac ggtcggcggg aggaagagcg gctgatccat cagggccgcg tagtcgttgc
     7561 cgccggaggc gcgggccgtc agcacgccgt cgctctgctg tgtccggtcg aacgacttcg
     7621 catcgagtgc gcgcaggacg ttcgacatgt attggacagg ctcgcgcaga cgcccatagg
     7681 ccgggtcggt cttcacgtcg ccgcgcgctt ccgggtcgag gaggacggag cgcacgacgg
     7741 cccccatctg gccgcgcgcg cccgtgcagc cgtctgggta gagcgcgtcg cagtcgttgt
     7801 tgaagacgcg ggccacgcgc tcgacgtagg ccggcgaggg gttgctggtg acgaggtgct
     7861 gaatgagctg cttgccgatg aagggggcga cgttcgggtg gttgaagatg ttgtcgagcg
     7921 ccgcgtcgag gtcggcctgc gcggtctgac cggcgggcag catcgccccg ccgagaagcg
     7981 tcttcgcgcc ctgatcgtga ttggcggcct gattggcgcg cggaaccatc gggtcgcggt
     8041 agttggtgac gccggcggcg atggccgggc cgaacgtcca gccggtgaag acgcgtgtga
     8101 actcgttgac cgtgttctgc gtgtagacgg ggatgcgcgc gccctgcgcg tcgaggcggg
     8161 gcgtgccgtc gagattcagc tcgtccacgc cggtggagaa gagttgcagc acctcgcgcg
     8221 cgaaattctc gttggggctg gtgcgcgtgc tgcggttcat gtcgaggtag tcgcccatcg
     8281 cgggggtgag cgtcacgtcg cggagcatct ggcggtagct gcccgtggcg ttgcggtcga
     8341 tggcctgaag atacggcgtc atccagctcg ggcgggggat gtcgcggccc gagacgacga
     8401 gcacctgatg gagggcgaag ccgacgcggt gtcggagttg actctggccg tacatcgcgt
     8461 tcgagaagaa gttcttctga agcgggtaca tcgaatagtt gtcgcggaag cagtcgcctt
     8521 gaagcgccgg cccgagcgag tcggggcact gctgcgccga ctgatcgttg gggaacagca
     8581 tgtctacgta attagtcgcg ggcgcgccga gctgctcgtt gacgtactgc cggaagccga
     8641 ttgatttgac gcgcgccacc tctccttcgg tcggccccca cgacgcctgc tcaagcagac
     8701 gaacggcgtc agcgtcggcc gtctcattcg gcccgtaagc cttcggctgg agcgcgggca
     8761 tcggcgtcgg cgtcgccgcc ggggcgggcg aaatcacttc ggccactgcg tcaacgggcg
     8821 gcccgccgcc cgcctgcccg agggccatgc ggacgcggtt cgacgagacg ccgtgaaggt
     8881 tgatgcggac gagcacgtcg ccgaggtcgt cggccatctc gtcgttgagg cgcaggacga
     8941 tggcgaagag ccatccctgc cccacgtcgc gat
//
LOCUS       DASVES010000122         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1197412, whole genome shotgun sequence.
ACCESSION   DASVES010000122 DASVES010000000
VERSION     DASVES010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197412"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1513
                     /locus_tag="VF240_04450"
     CDS             47..1513
                     /locus_tag="VF240_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7174518.1"
                     /translation="MITASSVGTMIEWYDFYIFGSLATVISPLFYPKGNETLALIAYL
                     STFAVGFVVRPFGALFFGRIGDLTGRKYAFLVTLLIMGGATALIGFLPTYATIGIAAP
                     IILLLIRVLQGLALGGEYGGAAVYVAEHVPDERRGFYTSFIQITATLGLFVSLAVILF
                     VQGAMSREAFAAWGWRIPFLISIFLVAVSLYIRLKMKESPIFTHVKSAGMTSAQPLKE
                     AFTKWPNLKRVLISLFGATAGQGVVWYTGQFYALFYLQTILKVHATSANYVVAVALLL
                     GMPFFVFFGWLSDRVGRKWIIMLGCLLAAISYLPLYRAMQSAAGSEVVSAHSTTDPVT
                     GSYKLTPQTIDAAGALQPAKEVLTFTDFATLIGDPVAWKLILLIFVQVIFVTMVYGPI
                     AAYLVEAFPAKIRYTSLSLPYHIGNGVFGGLLPLIGLSVVAQTGNIYAGLYYPIAVAL
                     VTFVVGSLLLRETRHVLIWDEVETATGKPLVSDIDGPV"
     gene            1682..>2266
                     /locus_tag="VF240_04455"
     CDS             1682..>2266
                     /locus_tag="VF240_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7174519.1"
                     /translation="MSDAAPDTQQTDIESTLHEERVFPPPAEFSERAHIGSMEEYERL
                     REEAARDPEAFWARMAEELHWFRRWDKVLSWEPPHAQWFVGGKTNVSYNCLDRHLSTH
                     RRDKVAFIWEGEPGDQRTLTYAELHREVCRFANVLKKLGIVSGDRVALYMPLVPELPI
                     AMLACARIGATHSVIFGGFSATAIIDRVNDAGCRL"
BASE COUNT          370 a          761 c          682 g          453 t
ORIGIN      
        1 ctggcgcccc gcacattccc gggaccatgc gaaggggatc tggaaagtga tcaccgcttc
       61 ctctgtcggg acgatgatcg agtggtacga cttctacatc ttcggcagcc tcgcgacggt
      121 catctcgccg ctcttttacc cgaaggggaa cgagacgctg gcgctcatcg cctacctctc
      181 gacgttcgcg gtgggcttcg tcgtgcggcc cttcggcgcg ctcttcttcg ggcgcatcgg
      241 cgacctgacg gggcgcaagt atgcctttct cgtgacgctg ctcatcatgg gcggcgcgac
      301 cgcgctcatc ggcttcctgc cgacctacgc gaccatcggg attgccgcgc ccatcatcct
      361 gttgctcatc cgcgtgctac agggactcgc tctgggcggc gagtacggcg gggcggccgt
      421 ctacgtcgcc gagcacgtgc cggacgagcg gcggggcttc tacacgtcgt tcattcagat
      481 cacggcgacg ctggggctct ttgtctcgct ggccgtcatc ctctttgtgc agggcgcgat
      541 gagccgggag gcgttcgcgg cgtggggctg gcgcatccct tttctcatct cgatcttcct
      601 cgtggccgtc tcgctctaca tccgtctgaa gatgaaagag tcgccgatct tcacgcacgt
      661 caagagtgcg gggatgactt cggcgcagcc cttgaaagaa gccttcacga agtggccgaa
      721 cctgaagcgc gtgctcatct cgctcttcgg ggcgacggcc gggcagggcg tggtgtggta
      781 cacggggcag ttctacgcgc tcttctacct ccagaccatc ctcaaggttc acgccacgtc
      841 ggccaactac gtcgtcgccg tcgcgctcct gttggggatg cccttcttcg tcttcttcgg
      901 ctggctctcc gaccgtgtcg gacgcaagtg gatcatcatg ctggggtgcc tgctcgccgc
      961 catctcctac cttccgctct acagggcgat gcagagcgcc gccggctcgg aggtcgtcag
     1021 cgcgcactcg acgaccgacc ccgtcaccgg ctcttacaaa ctcacaccgc aaaccatcga
     1081 cgccgcgggc gcgctgcaac ctgctaaaga agtcctgacc ttcacggact tcgcgacact
     1141 catcggcgac cccgtcgcgt ggaagctgat tctgctcatc ttcgtgcagg tcatcttcgt
     1201 gacgatggtc tacgggccga ttgcggccta tctggtcgaa gccttcccgg cgaagatcag
     1261 gtacacgtcg ctgtcacttc cctatcacat cggcaacggc gtcttcggcg gactcctgcc
     1321 gctcatcggc ctgtcggtcg tggcgcagac cggcaacatc tacgccggcc tctactaccc
     1381 catcgccgtc gccctcgtca cgttcgtcgt cggctcgctc ctgctcaggg agacacggca
     1441 tgtgctcatc tgggacgaag tggagacggc gacgggcaaa cccctcgtgt cggatatcga
     1501 cgggccggtt tagagtgatg ttcacttgtg tgaaagggga atagattcac cgcgctgagc
     1561 cgactgacca tacactcaag ttataaacga catagtgccg cgggcgcgct gatgtccctc
     1621 accggttgcc tcgtcgtata atgccgggca cgactttgag ccaagaggaa gaaaagtttt
     1681 tatgtctgac gccgcgcctg acacgcaaca gaccgacatc gaatccacgc tgcacgagga
     1741 gcgcgtgttt ccgccgcccg cggagttttc cgaacgagcg cacatcgggt cgatggagga
     1801 gtacgagcgt ttgcgcgagg aggccgcgcg cgaccctgaa gcgttctggg cgcgcatggc
     1861 cgaggagctg cactggttca ggcgttggga caaggtgctt agttgggagc cgccgcacgc
     1921 ccagtggttt gtcggcggca agacgaacgt ctcctacaac tgtctcgacc gacacctctc
     1981 gacgcaccgg cgcgacaagg tcgccttcat ctgggaaggc gagccgggcg atcagcgcac
     2041 gctcacctac gccgagctgc accgagaggt ctgccgcttt gcgaacgtcc tcaagaagct
     2101 cggcatcgtg agcggcgacc gcgtcgccct ctacatgccg ctcgtgccgg aactccccat
     2161 cgccatgctc gcctgcgcgc gcatcggcgc cacccactcc gtcatcttcg gcggcttctc
     2221 cgccacggcc atcatcgacc gcgtcaacga cgccggctgc cgcctc
//
LOCUS       DASVES010000123         4882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_151303, whole genome shotgun sequence.
ACCESSION   DASVES010000123 DASVES010000000
VERSION     DASVES010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151303"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VF240_04460"
     CDS             complement(<1..282)
                     /locus_tag="VF240_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174520.1"
                     /translation="MRSLETSEEFQLMSDATQTKPDGAAKGAGRRSTLADYAPTYGAF
                     AALLLLLVIDSIFIPNFFHPNNFRNILLQIAPTMLVATGMTFVIATGGID"
     gene            complement(463..2019)
                     /locus_tag="VF240_04465"
     CDS             complement(463..2019)
                     /locus_tag="VF240_04465"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7174521.1"
                     /translation="MNQESLLRMEGITKVFSGHVALDGVDFRLGRGEVHALVGENGAG
                     KSTLIKIMTGAYRRDGGRVWLEGREVSFDSPEDAQSEGVVAVYQEVNLLMFRTVAENI
                     FLGREPRRFRLVDWKRMNADADAILKRLGLEIDPRATLGSLNIALRQMVAIARGVSFG
                     AKVVVLDEPTSSLTEREVSILYDVIRRLKAEGVGVVYISHRFDELYTVCDTVTVLRDG
                     KFVATRALEGLEKIDLVCLMLGKQREELQKKGATAFAGQHEAAPEGSVPLLRAENLTR
                     GRKLNRVTLDAGRGEIVGMAGLLGSGRTETARAIFGADAVDEGTVELEGKRLRLGSPG
                     EAIDAGLAFLSEDRKAEGIIPELSVRENMTLAALPSLSSAGVVSRSKQNEVVERFMRR
                     LGIKASSAEQKIRELSGGNQQKVLLARWLCKNPKFLILDEPTRGIDIGAKGEIQSLIN
                     ELASEGLGVLMISSELEELVEGSSRVVVMRDGRAVAELRGGEISQDSIIHAMAEGGEA
                     KDEEERAEEL"
     gene            complement(2103..3131)
                     /locus_tag="VF240_04470"
     CDS             complement(2103..3131)
                     /locus_tag="VF240_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7174522.1"
                     /translation="MKSFHIGRRITFRALLLVALLALAFAGCRKEGSGGSVGGGNTAA
                     GGGAKKEGKLRVGFSQMEHSGPWRIAETQSMKDEAAKRADRYELIVTDAQGQTAKQVS
                     DVEDLVAQRVDAIFLAPREAVGFELALQAAKDAGIPVFLIDREVRGEAGKDFVAFIGS
                     NFVEEGRRAGEWLAKQTNGKAGIVELLGSAGSSVADDRHDGFAEAIKAHPDMRIIASQ
                     PGNFTRAEGQKVMENLIQAHGPNITVVYAHNDEMALGAIQALKGAGKQPGKDVLVVSV
                     DGQKSALEAIARGEMNATVECNPRFGPIAFDMLEKYLRGEKIDPKIIVPDRFFDASNA
                     AQFVGEAY"
     gene            complement(3172..>4882)
                     /locus_tag="VF240_04475"
     CDS             complement(3172..>4882)
                     /locus_tag="VF240_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX7174523.1"
                     /translation="LNEEEGKQMRKMEFRRLTFVGKPVRLMRRVRRVGRGRAGSAALA
                     LALLLALSVGAAGQRRAVYTNPVAAGDYPDPSIIRVGTDYWATATTSEWAPEFPILHS
                     RDLVNWEVVGAVFDRRPEWSVGSYWAPEISEHRGRFYVYYVARKKNGPLCVAVATARR
                     PAGPYRDHGPLVCQEVGSIDGFPVTDERGRRYLLWKEDGNSVQKPTPIWAQPLSADGT
                     RLVGERQEILRNTEPWEKHEKLPYGDLVEGPAVVRRGGWFYMFYSGNFCCARECNYMM
                     GVARARRLLGPWEKHPGPILGGNADWKCPGHGTVVQDARGRDWMLYHAMHAKDSVYVG
                     RQAVLDEVKWGADGWPTINEGRGPSTRADAPPGARERNAEYSFFDDFNSPRLRQGWQW
                     PVANEPIVRIDAARQGWLTLSPNPSAQPAAAASVAILARPTTVGDYEATTVVDTARLT
                     GAAAAGLSAYGDAENAVGLIVGGGKLTLWRREKNEQRVVETVDAPAGAASLHLRMRAR
                     EGRRYRFAYSRDGRDWKDVGGEVDGEHLPPWDRGVRVALVAGGAPNSGAARFGFLRVE
                     PSR"
BASE COUNT          773 a         1663 c         1572 g          874 t
ORIGIN      
        1 gtcgatgccg cccgtggcga tgacgaaggt catgccggtg gcgacgagca tggtcggcgc
       61 gatctggagg aggatgttgc ggaagttgtt cgggtggaag aaattcggga tgaagatcga
      121 gtcgatgacg agcagcagca gcagcgcggc gaacgccccg taggtcggcg cgtaatcggc
      181 cagagtcgag cgccggccgg cgcccttcgc cgccccgtcc ggtttggttt gcgtcgcgtc
      241 cgacatcaac tggaactcct ccgaagtttc cagacttctc aatgaatgat ttctgtaggg
      301 gcagggcttg tccctgcatg tacttacaga agaattaaca tggatgggca ggatagatag
      361 gataagaaga tttgaaatct gaaatctgaa atctcaattc agctctttct tcatcctgac
      421 tatcttgtcc atccatgtta atttctttcg cgacctgccc ccctaaagtt cttccgcgcg
      481 ctcttcttcg tccttcgcct cgccgccctc ggccatcgcg tggatgatgc tgtcctgcga
      541 aatctcgccg ccgcgcagct cggccacggc ccggccgtcg cgcatgacga cgacgcgcga
      601 gctgccctcg accaactcct ccagctccga ggagatcatc aacacgccca gaccctccga
      661 ggccagctcg ttgatgagcg attgaatctc gcccttcgcg ccgatgtcga tgccgcgcgt
      721 cggctcgtcg aggatgagga acttcgggtt cttacagagc cagcgcgcga gcagaacctt
      781 ctgctggttg ccgcccgaaa gctccctgat cttctgctcg gcgctcgaag ccttgatgcc
      841 gagccgccgc atgaatcgct cgacgacctc gttctgcttc gagcgcgaga cgacgcccgc
      901 gcttgagagc gacggcagcg ccgccagcgt catgttctcg cgaacagata gctcggggat
      961 gatgccctcg gccttgcggt cttcggagag gaaggctaac ccagcgtcaa tggcctcgcc
     1021 gggcgagccg agccgaagcc gcttgccttc gagttcgacc gtgccctcgt ccaccgcgtc
     1081 agccccgaag atggcgcggg ccgtctcggt gcgccccgag ccgagcaacc ccgccatgcc
     1141 gacgatctcg ccgcggccgg cgtcaagcgt tacacggttg agcttgcggc ctcgcgtcaa
     1201 attctcggcg cgcagtagag gcacggagcc ttccggcgcg gcctcgtgtt ggccggcgaa
     1261 ggccgtcgcc cctttcttct gcaactcctc gcgctgcttg ccaagcatga ggcagacgag
     1321 gtcgatcttc tccaaccctt cgagcgcgcg cgtggcgacg aacttgccat ctcggagcac
     1381 ggtcactgtg tcgcagaccg tgtagagttc gtcgaagcgg tggctgatgt agacgacgcc
     1441 gacgccctcc gccttgaggc gtcgaatcac gtcgtagagg atggagacct cgcgctcggt
     1501 gagcgaggag gtcggctcat ccaggacgac gaccttcgcg ccgaaggaga cgccgcgcgc
     1561 aatcgccacc atctgccgga gcgcgatgtt gaggctcccc agagtggcgc gcggatcgat
     1621 ctccagcccg aggcgtttga ggatggcgtc ggcgtcggcg ttcatccgct tccagtcaac
     1681 cagacggaaa cggcgcggct cgcgcccgag gaagatgttc tcggccacgg tgcggaacat
     1741 cagcaggttg acctcctgat agacggcgac gacgccctcg ctttgcgcgt cctccggcga
     1801 gtcgaagctc acctcgcgcc cttcgagcca cacgcgcccg ccgtcgcggc ggtaagcgcc
     1861 cgtcataatc ttaatcagcg tggacttgcc cgcgccgttc tcgcccacga gcgcgtgcac
     1921 ctcgccgcgt ccgagacgaa agtccacgcc gtcaagcgcg acgtggcccg agaagacttt
     1981 cgtgatgccc tccatgcgga ggagagactc ttgattcata gttcaaagtt aaaagttcaa
     2041 agttcaaagt caacagccgc cccgtgcgct ttgaactttg aactttgaac cttgaactcc
     2101 tttcagtatg cctcgcccac gaactgcgcc gcgttcgacg cgtcgaagaa gcggtcgggg
     2161 acgatgatct tggggtcgat cttctcgccg cgcaggtatt tttcgagcat gtcgaaggcg
     2221 atggggccga agcgcgggtt gcactcgacg gtggcgttca tctcgccgcg cgcgatggct
     2281 tcgagcgccg acttctgccc gtccaccgag acgacgagca cgtccttgcc cggctgctta
     2341 cccgcgccct tgagggcttg aatggcgccg agggccatct cgtcgttgtg cgcgtagacg
     2401 accgtgatgt tggggccgtg cgcctgtatg agattttcca tcaccttctg cccctcggcg
     2461 cgcgtgaagt taccgggttg cgaggcgatg atacgcatgt cggggtgcgc cttgatggcc
     2521 tcggcgaagc cgtcgtggcg gtcgtcggcg acagacgagc cagcgctgcc gagtagttcg
     2581 acgatgccgg ccttcccatt cgtctgcttg gcgagccact cgccggcgcg tcggccctcc
     2641 tcgacgaagt tcgagccgat gaaggcgacg aagtctttgc cggcctcgcc cctgacctcg
     2701 cggtcgatga ggaagacggg gatgcccgcg tccttcgccg cctgaagggc aagctcgaag
     2761 ccgacggcct cgcgcggggc gaggaagatg gcgtctacgc gctgcgcgac caagtcttcc
     2821 acgtccgaga cctgcttggc cgtctgcccc tgcgcgtcgg tgacgatcaa ctcgtagcgg
     2881 tcggcacgct tcgccgcctc atctttcatg ctctgcgtct cggcgatgcg ccacggcccg
     2941 ctgtgctcca tctgcgagaa gccgacgcgt agcttccctt cctttttggc gccgccgccc
     3001 gcggccgtat tgccgccgcc caccgacccg ccgctgccct ccttgcggca gcccgcgaag
     3061 gcgagcgcga ggagcgcgac gagcagcagg gcgcggaagg tgatgcgccg tccgatgtgg
     3121 aacgatttca tgttgtcctc tccggtgtgt tgattagtcc gtgaataaaa cttaacgcga
     3181 aggctcgacg cgcaggaagc cgaagcgggc cgcgccgcta ttgggtgcgc cgcccgcgac
     3241 gagcgcgacg cgcacgcccc tgtcccacgg cggcaggtgc tctccgtcaa cctcgccgcc
     3301 cacgtccttc cagtcgcgcc cgtcgcggct gtaggcgaag cgatagcggc ggccttcgcg
     3361 cgcgcgcatc cgcaggtgga gcgacgcggc cccggcgggc gcgtcaaccg tctcgacgac
     3421 gcgctgctca ttcttctcgc gccgccacaa ggtcaacttg ccgccgccca cgatcaggcc
     3481 cacggcgttc tccgcgtcgc cgtaggccga cagcccggcc gcggcggcgc ccgttagtcg
     3541 cgccgtatcg acgacggtgg tcgcctcgta gtcgccgacg gtggtcgggc gcgcgaggat
     3601 ggcgacggag gccgccgccg cgggctgggc ggacgggttc ggcgacagcg tgagccaccc
     3661 ttgtcgcgcc gcgtcgatgc ggacgatagg ctcgttggcc accggccact gccagccctg
     3721 gcgcaggcgc ggggagttga agtcgtcgaa gaaactgtac tcggcgtttc tttcgcgcgc
     3781 gcccggcggc gcatcggcgc gcgtgctggg gcctcgccct tcgttgattg tgggccagcc
     3841 gtcggcgccc cacttcacct cgtcgagcac ggcctgccgg ccgacgtaga cggagtcctt
     3901 cgcgtgcatg gcgtgataca gcatccagtc gcgccctcgc gcgtcctgta cgacggtgcc
     3961 gtggccgggg cacttccagt cggcgttgcc cccgaggatc ggccccggat gcttctccca
     4021 cgggccgagg agccggcgcg cgcgggcgac gcccatcatg tagttgcact cgcgcgcgca
     4081 gcagaagttg cccgagtaga acatgtagaa ccaaccaccc ctgcggacga cggccgggcc
     4141 ttcgacgagg tcgccgtagg gcagcttctc gtgcttctcc cacggctcgg tgttgcgcag
     4201 aatctcttgc cgctctccga cgaggcgcgt gccgtcggcc gagagcggct gcgcccagat
     4261 gggcgtcggc ttctggacgg aatttccgtc ctccttccac agcaggtagc gacggccccg
     4321 ctcgtcggtg acggggaagc cgtcgataga gccgacctcc tggcagacga gcgggccatg
     4381 atctctgtac ggccccgccg gccggcgggc ggtggcgacg gcgacgcaga gcggcccatt
     4441 cttcttgcgc gcgacgtagt agacgtagaa gcgcccgcgg tgctcggaaa tctccggcgc
     4501 ccaatagctg cccacagacc attcggggcg gcggtcgaag acggcgccga ccacctccca
     4561 gttaacgagg tcgcgcgagt ggaggatggg aaactccggc gcccactccg aggtcgtcgc
     4621 cgtcgcccag tagtccgtcc cgacgcggat gatcgacggg tcggggtagt ctccggccgc
     4681 gaccgggttc gtgtagacgg cgcgccgctg ccccgccgcg ccgacactga gggcgagcag
     4741 cagcgccagc gccagcgccg cactgccggc ccgtccccta cccacccgcc gcacacgccg
     4801 catcaggcga acgggtttcc caacgaacgt gagccgtctg aactccatct tccgcatctg
     4861 cttcccctcc tcctcgttca at
//
LOCUS       DASVES010000124         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_858392, whole genome shotgun sequence.
ACCESSION   DASVES010000124 DASVES010000000
VERSION     DASVES010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858392"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /gene="ggt"
                     /locus_tag="VF240_04480"
     CDS             complement(<1..1751)
                     /gene="ggt"
                     /locus_tag="VF240_04480"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7174524.1"
                     /translation="MKTARTLIILLLAGTFLHAPHKMSVLPGHEAVARAAARQPVRAP
                     RGMVASTTRISSQVGVDVLKRGGNAVDAAIAVALALAVTYPAAGNLGGGGFMLIRMKD
                     GRTTAIDYREMAPAAAQRNVYLDDKGELIKGEGSSTVGYRAAGVPGTVAGMELALRKY
                     GSGRLTWAQLVEPSRRLAAEGFPVGYRLARNLRASRELLEGYADSKKIYLNGGKFYDE
                     GDILRQPELAATFARLQRRGPREFYEGQTARLIADDMKRNNGLITLEDMSNYVAKERT
                     PLSATYRGHEVLSMPPPSSGGAVLIEMLNILEAYDLRALGWSSSERYHLTTEAMRRAF
                     ADRAEYMGDADFVDVPVAGLVDKTYAERQRASIRLDRASTSAEVCAGNPPGVAANTKK
                     EVCPTRPTGRESEETTHFTVVDAEGNAVSNTYTLNLGYGSGVTARGTGVLLNNEMDDF
                     AAKPGTPNAFGLIQSEKNAVGPRKRPLSAMTPTFVMRKDGTLWFTIGSPGGPTIINTV
                     LQVISNVVDFDMNIQQAIDAPRIHHQWLPDEVVYEPYGLSADTYRALLGRGHKVTEKP
                     RYMGDAQGVMIEEGTNVR"
     gene            complement(1870..2340)
                     /locus_tag="VF240_04485"
     CDS             complement(1870..2340)
                     /locus_tag="VF240_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174525.1"
                     /translation="MAAIQITAALTEQDILLARELFVEYADATGLDLCFQGFERELAE
                     LPGDYAPPAGRLFLARVDGREAGCVALRRFEEGVCEMKRLYVRPGFRGFRLGRRLTEL
                     LIEQAHGAGYERMRLDTLPSMQEAIRLYRSLGFVEIAPYRFNPVGGTLYFELCL"
     gene            complement(2475..>2823)
                     /locus_tag="VF240_04490"
     CDS             complement(2475..>2823)
                     /locus_tag="VF240_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174526.1"
                     /translation="DEAHRIDGLIRASEPFDGRAKLRVDGGINRPPLERTERVAALYE
                     AARCSASLFDFELGEASVGGASDGNFAAAVGAAVLDGLGVEGDGAHAAHEHIIADALP
                     LRGALLTALVASL"
BASE COUNT          486 a          932 c          910 g          495 t
ORIGIN      
        1 cgcacgttcg tgccctcctc gatcatcacg ccctgcgcgt cgcccatgta gcggggcttc
       61 tccgtcacct tgtggccgcg ccccagaagc gcgcggtaag tgtcggccga aagaccatac
      121 ggctcgtaga cgacctcgtc cgggagccac tggtgatgga tacgcggcgc gtcaatcgcc
      181 tgttggatgt tcatgtcgaa gtccacgacg ttcgagatca cctgaagcac ggtgttgatg
      241 atcgtggggc cgccggggga gccgatggtg aaccagagcg tgccgtcctt ccgcatgacg
      301 aaggtgggcg tcatcgccga gagcgggcgc ttgcgcgggc cgacggcgtt cttctcgctc
      361 tggatgaggc cgaaggcgtt cggcgtgccg ggcttagcgg cgaagtcgtc catctcgttg
      421 ttgaggagga cgcctgtccc gcgcgccgtc acgccggagc cgtagccgag gttgagcgtg
      481 taggtgttgg agacggcgtt gccttcggcg tcaaccaccg tgaagtgtgt ggtctcctcc
      541 gactcgcggc ccgtcggacg ggtcgggcac acttccttct tcgtgttcgc cgcgacgccg
      601 ggaggattgc cggcgcacac ttcggcgctc gtcgaggcgc ggtcgagacg gatggaggcg
      661 cgctggcgct ccgcgtaagt cttatcgaca agaccggcga cgggcacgtc cacgaagtcc
      721 gcgtcgccca tgtactcggc gcggtcggcg aaggcgcgtc gcatcgcctc ggtcgtcaga
      781 tggtaacgct cagaagaaga ccagccgagc gcgcgcaggt cgtaggcttc gaggatgttg
      841 agcatctcga tgaggacggc gccgcccgac gaagggggcg gcatcgagag cacctcgtgg
      901 ccgcggtagg tggcgctgag cggcgtgcgc tccttcgcca cgtagttgct catgtcttcg
      961 agagtgatga ggccgttgtt gcgcttcatg tcgtcggcga tgaggcgcgc ggtctgtccc
     1021 tcataaaact cgcgcggccc gcgccgttgc agccgcgcga aggtggcggc cagctcgggc
     1081 tgccgcagga tgtcgccctc gtcgtaaaac ttcccgccgt tcaggtagat ttttttcgag
     1141 tcggcatagc cctcaagcag ttccctgctc gcgcggaggt tgcgagcgag tctgtatccg
     1201 acgggaaaac cttcggcggc cagacggcgc gaaggctcaa caagctgcgc ccacgtgaga
     1261 cggcccgagc cgtacttcct gagcgccaac tccatgccgg cgaccgtgcc ggggacgccc
     1321 gccgcgcggt agcccaccgt cgatgagcct tcgcctttaa tcaactcgcc cttgtcatcg
     1381 agatagacgt tgcgctgggc cgcggccggg gccatctcgc ggtagtcgat ggccgtcgtg
     1441 cgcccgtcct tcatgcgaat caacatgaag ccgccgccgc cgagattgcc ggccgccggg
     1501 taggtgaccg cgagcgcgag cgccacggcg atggctgcgt ccacggcgtt gccgccgcgc
     1561 ttcaaaacat cgacgccgac ctgcgaagag attctggtgg tggaggcgac catcccgcgc
     1621 ggcgctcgaa ccggctggcg tgcggcggcg cgggcgacag cctcgtgccc cggcagcacc
     1681 gacattttgt gaggcgcgtg aaggaaagtt ccggcgagca ggaggatgat taaagtgcgt
     1741 gcggttttca tgtgcctctt tttcccgtga gaacaattcc gaactgcctg acttgaaagt
     1801 cgcggggcct gtggtcgcgc cgtggatttt atcagatgaa cgcctttcga catcaacccc
     1861 gcgctgcccc tacaggcaaa gctcgaaata gagcgtcccg cccaccgggt tgaaacggta
     1921 aggggcaatc tccacgaagc ccagcgaacg atagagccga atggcctctt gcatggacgg
     1981 cagcgtgtcc agacgcatcc tctcgtaccc ggccccatgc gcctgctcga tcaacaactc
     2041 cgtgagcctc cgccccagcc gaaatccccg aaaaccgggc ctcacataca ggcgcttcat
     2101 ctcgcagact ccctcctcaa atcttcgcag cgccacgcac cctgcctcac gaccatccac
     2161 acgcgccaga aaaagacgcc ccgccggcgg cgcataatcg cccggcaact cggctaactc
     2221 gcgctcgaag ccctgaaagc acaaatctaa acccgtggca tcggcgtact caacgaacaa
     2281 ttcacgcgca agtaatatgt cctgttccgt gagtgctgct gtgatttgaa tggctgccat
     2341 gtgcaaacgg ccgacggttg ttgccgagcc ttatcaccac aaacagtacc gcgagcggta
     2401 gcgagcgggt gcgacctgcg atgagaggcc agcctcgctc gcccagaccc gcgaggcttc
     2461 cgctcgcggt actgttatag cgaggcgaca agggcggtga gcagtgcgcc gcgaagcggc
     2521 agggcgtcgg cgatgatgtg ctcgtgcgcg gcgtgtgcgc cgtcgccttc aacgccgagg
     2581 ccgtcgagca cggctgcgcc cacggccgcc gcaaagtttc catccgacgc tccgccgacg
     2641 ctcgcctccc ccagctcgaa atcaaacagc gacgccgagc agcgcgccgc ctcgtagagc
     2701 gcggcaaccc gttccgtcct ctccagtggc ggccggttga ttccgccgtc cactctcaac
     2761 ttggcccgcc cgtcgaacgg ctcggaggcg cggataagac cgtcgatcct gtgcgcctcg
     2821 tcg
//
LOCUS       DASVES010000125        14765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1695559, whole genome shotgun sequence.
ACCESSION   DASVES010000125 DASVES010000000
VERSION     DASVES010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14765
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695559"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF240_04495"
     CDS             complement(<1..317)
                     /locus_tag="VF240_04495"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7174527.1"
                     /translation="MTKEVFLTAALRTPIGRFGGALAGMTAADMGVVVARESLRRAGL
                     ETSGVQEVLWGCARQAGGGPNVARQIAYRAGLPETIPAATINQACGSGLKAIILAAQE
                     IMLG"
     gene            480..2018
                     /locus_tag="VF240_04500"
     CDS             480..2018
                     /locus_tag="VF240_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174528.1"
                     /translation="MNRLRNPSEIETAARRIAALLDARGEWFCAEGRGAERPSALRRG
                     EWEVSAQRGALLLSYWGAGGARVWRVRGWEWRGGKLLLEATRRAGAVRATLELVPRAS
                     AAADAAELAAARRAECERLAALLCESVGPGARIEEARLSAGARRGEPGRYARILLRRG
                     RETHAATGPIVELGSEEAGAFVASALLWFAQLGSRRAGAPRGLLLFGPRKLSAAAAEL
                     VSLLRAELRGALEVYELGGGRKTFERVREPDLEELLDASPRLSGPARADLTETAARIR
                     GLAPEAIDVVRARHGETLRFRGLAFARVRRLPGGERAWFGVEGVRQRRSLDASSWPDL
                     PRLVGELEEHRRAGGSAGRHALYRAAPEAWLESLLRRDVTRLDPGLVVSPVHAQFRTT
                     RGQRARGGPIDLLALRRDGRLVVIELKVSEDAALPLQGAQYWRRISTHHRAGHITRAR
                     LFGNAEISDDPPLVYLVAPLLRFHRSFQTLARAVTPEIEMYRFDLNEDWRAGVRVARR
                     ARIG"
     gene            2210..4399
                     /gene="katE"
                     /locus_tag="VF240_04505"
     CDS             2210..4399
                     /gene="katE"
                     /locus_tag="VF240_04505"
                     /inference="COORDINATES: protein motif:HMM:NF008422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HEX7174529.1"
                     /translation="MGKGKQKNDDKGVNGKVSDLAKNTEDGTDEYLTTNQGLRINDNQ
                     NSLKAGERGATLLEDFILREKITHFDHERIPERIVHARGAAAHGVFQVYESMAEYTKA
                     GFLQNPIVKTPVFVRFSTVAGSRGSTDLARDVRGFAVKFYTEEGVYDLVGNNIPVFFI
                     QDAIKFPDLVHAVKPEPHNEIPQAASAHDTFWDFISLMPESMHMIMWAMSDRAIPRSF
                     RMMEGFGVHTFRFINAKGKARFVKFHWKPLLGVHSVVWDEAKKISGNDPDFHRRDLWE
                     AIENGDYPEWEFGVQIVEEKDEHKFDFDLLDPTKIIPEELVPVRRIGKLTLNRNPDNF
                     FAETEQVAFHPGHIVPGIDFTNDPLLQGRLFSYTDTQLKRLGSPNFHEIPINRSVAPV
                     HNNQRDAHMRQTINVGRVAYEPNTLGGGCPFQAGADAGGFTSYAEKIDARKVRARSES
                     FFDHFSQATLFYNSQSAPEQEHIVNALRFELGKVETPAIRERMVYVLTHIDKTLAGRV
                     AEGLGIKVPVRIDTPLNMSVPADGDPKKFQPKREVKEVGSSPALSMANTVKDTVKTRK
                     VAFLAADGFDDDALAAMKKALAGAGAQVKIVAPRLGFLKGSGGAEVKIDFSFLTCASV
                     LFDAVYIAGGEKSAAALKGEVDAIHFVNEAFKHCKAIAATGAGVEVLRASSIGAGKAD
                     SGGGKVVSDEGVVTGQDTQAGKAAAEFIKAIAQHRHWSREAKAQVPA"
     gene            complement(4561..5301)
                     /locus_tag="VF240_04510"
     CDS             complement(4561..5301)
                     /locus_tag="VF240_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ran-binding zinc finger domain-containing
                     protein"
                     /protein_id="HEX7174530.1"
                     /translation="MNSKCPSCGLVNFSTAHECKRCGALLSAEFAPRAADEFARLGQG
                     ESAAADFAEFEEEARPRRSLMRKVLAGCVTALLLLVVFYVSLLETSTAVTYEQKQVVQ
                     RAVDLIERKGFHQDAFLLRRLANFRTSDNWWNNYLGHADAYAATNFPFEVVTLYPEFF
                     TRPKDDTERAVILLHEARHLAGSGEEAAFRSVWRDKSRLGYTYDKYTDSQVYLNVVEY
                     TMKYAPELFRCGPDGRADCLAQNSRPQQ"
     gene            complement(5479..6633)
                     /locus_tag="VF240_04515"
     CDS             complement(5479..6633)
                     /locus_tag="VF240_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX7174531.1"
                     /translation="MSIKINDLLKTATQSGASDLHVKAGSQPFMRVSGELRALAETPR
                     LTQEDTLDMAFSIMSNRQKQHFKEASEVDIAYGVTGLGRFRVNIFQQRGTVGMVLRVI
                     PDRMRSMTELSLPPVIERIGDEQRGLILVTGTTGSGKSTTLAAMIDHINSTRGGHIVT
                     IEDPIEFLHRDKRSFVTQREVDVDTRSFSEALRGALRQDPDVILVGEMRDYETIETAL
                     SAAETGHLVLSTLHTLDATETIRRIVSTFPPHLQKSVRIQLAGILKAVISMRLIPAAR
                     GGGRVPAVEVMVSTGLIRDYIVNEEKTSLIRDAIAAGTSQYGMQTFDQSLFNLYQSGL
                     IPLEEALRGASNADEFRLRIAGIRSASDQAREDMERVTTPSTYVRDAADD"
     gene            complement(6899..7978)
                     /locus_tag="VF240_04520"
     CDS             complement(6899..7978)
                     /locus_tag="VF240_04520"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX7174532.1"
                     /translation="MFNDDGPKKDVKTARIVPEIWHNGQFIKWDDARVHVMSHVLHYG
                     SSVFEGIRCYPTGRGPAIFRLREHMQRLVNSSKIYRMDHEWSVDELCEAAVGLVARSG
                     FEQCYVRPIIFRSLDEERPAFGVNPFPNPLSCYIGAWDWGKYLGEEAIEEGVDVCVSS
                     WNRLSPNSMPVMAKSGANYMNSQLIKMEAIKNGYAEGIALDDRGFVSEGSGENIFLVS
                     NGKVITPPLSSSILPGITRDSVIQICRELEIPVVESGLQRAALYIADELFFTGTAAEV
                     TPIRSVDKIKIGEGRRGPITAQLQKAYFEVTKGERPAPGPWLTFVNEHKAAAASEAGN
                     GFHPEQPRPAEKAAEPVTFESVMTE"
     gene            8280..9566
                     /locus_tag="VF240_04525"
     CDS             8280..9566
                     /locus_tag="VF240_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX7174533.1"
                     /translation="MRQVLFILHPSSLILVFALAGCRQQATTDNTNMPDPTPQTNATA
                     TAADKAADKESDALSIHRRALSIDMHADTVQFILDEGADISASVPGLHLDAPRMRAGG
                     LDAQFFSIWIEPSYYGAGGQLAVERADEQVAAVRALAEARPDVWALATTADEVRRAAG
                     EGRLAALMGLEGGYALDERLENVEKYYRAGVRYLSPTWSVSLAWAGSSGDAAGQSRGL
                     SDFGRRVIGEMNRLGIMVDVSHVSDKTFWDIIDASSKPVIATHSNARAIAPVARNLSD
                     DMIRAIAGKGGVVCVVFYPQFIEPGWEEKKLRVDAEIAGMVGAASERAVGRGSARRIA
                     RDRVREREYARRLPPVSAARVVDHIDHIVRLVGVAHVGVGSDFDGIQAVPSDLSSVAD
                     LPNLTAELLRRGYKKEDIHKILGGNILRVMAEVEKH"
     gene            9820..10458
                     /locus_tag="VF240_04530"
     CDS             9820..10458
                     /locus_tag="VF240_04530"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7174534.1"
                     /translation="MANGTVVIDPGHGGSVEIGGSSANNAISASGVLEKNITLRMAFL
                     VRDALKELATLGGHTLQIHLTRDTDKNLGLCDRAKVAKTKSANIFLSIHCNGFDKTVR
                     GTETLIRLAASNPKHAADKALAQRVQKAVFNAIKSHDANAKNRGVKDQLLGVLNRNCL
                     GSKPRGCMVEMEFIDVPAVDKLLNIGPNSPQVRSDIAKAIAGAIIDDLIANP"
     gene            10560..11036
                     /locus_tag="VF240_04535"
     CDS             10560..11036
                     /locus_tag="VF240_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174535.1"
                     /translation="MKCPALFARRGTMWLALSVACLSPLALAGCSRSQPAASNSPAAA
                     HQTPAATPSRAYTPVFARREAAAAGQKRGTLGDDFQWAAGADTLEEAAARWEDFLKTH
                     APAGAAGAAGEEYEDAFQANHVSSARYELMRVYYLLGRAGDGDRLLRELDPLSLSN"
     gene            11146..11403
                     /locus_tag="VF240_04540"
     CDS             11146..11403
                     /locus_tag="VF240_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7174536.1"
                     /translation="MNIELKERSEGPYYLQVRTQIETLIREQKVAAGDALPSPAQLAQ
                     QLAIDRGEVQRAYFELEQAGLISKKVGRDFLGKEKVTYAVK"
     gene            11739..14309
                     /gene="leuS"
                     /locus_tag="VF240_04545"
     CDS             11739..14309
                     /gene="leuS"
                     /locus_tag="VF240_04545"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7174537.1"
                     /translation="MDEKYFAEVVEEKWQRHWKESGAFEVERDDARARFYCLEMLPYP
                     SGYLHMGHVRNYSIGDALSWYKRLRGFNVLHPIGWDSFGQPAEQAAIKRGVNPKDWTE
                     ENIAHMRGQLQRLGVSYDWRREIAAHTPEYYKWDQWFFLKMLERELAYKRLSPVNWCP
                     TCQTTLSNEQSSGGVCWRCGNPVERRDIEQWFLRITAYAEALLEDMKEIEGAWPERVL
                     TMQRNWIGKSTGAFVDFQIKDGEDKIRIFTTRVDTIYGATAVVVAAEHPVVERMLAEG
                     GPSALKDEVRAFAERVRATHGTSVKVAGEEQEEKEGINTGLIAINPFSGEQAPVWVAN
                     YVLMEYGTGAVMSVPAHDERDYEFAQKYSLPIRRVIERISHEQPVIEHDPDHEDQSVA
                     MHQAFTEYGILVNSADWSGKLSEDATREMAEFARESGFGEPATTFRLRDWGISRQRFW
                     GAPIPIIYCERCGAVPVPYEDLPVRLPERVEFGATGQSPLAGVEEFVNVSCPKCKGAA
                     RRETDTMDTFVDSSWYFFRYCDPRNTEAPFDSETARYWTPVDQYIGGIDHAVMHLLYT
                     RFWTKAMRDMGLVNFGEPVRRLMTQGMVTNLIGETGEWKRMSKSRGNGVDPDDMIQAY
                     GSDAVRLFILFAAPPESELRWQETGIDGAVRFLRRVYSMVYRWRDALRDAPVEAPSAD
                     DFTTPARKLRHETHRTIARVTDDFERLHLNTNVAALMELSNALGDFQTKPEGATAAEL
                     FAAREALEALVLMLAPFAPHAAEEMWEGLGHEGGLLGGGRALWPSADPALARKEELEI
                     PVQVNGKLRGRLTVGADASEDELRSAALADERVRAWVEGKEIVKVVVVPKRLVNIVVR
                     "
BASE COUNT         2646 a         4630 c         4955 g         2534 t
ORIGIN      
        1 cccagcatga tctcctgcgc ggcgaggatg atcgccttca gacccgagcc gcaagcctga
       61 ttgatggtcg cggcagggat agtctcgggg agtcccgcgc ggtaggcgat ctgccgcgcg
      121 acgttcggcc cgccgcccgc ctgtcgcgcg cagccccaca acacctcctg cacaccgctc
      181 gtctcaagac ccgcgcgccg cagactctca cgcgccacga ccacgcccat gtccgcggcc
      241 gtcattcccg cgagcgcgcc gccgaaccta ccgatgggcg tgcgtagcgc ggccgtcaga
      301 aaaacctctt tcgtcatgct tcccctcagt cgtgtttgat ggtcgtttcg gactttcgtc
      361 aacgatgtta acatacgcgc cgacggcccg gcgacgacgc ctcacgcgcg ttcgcgcgtc
      421 gcttcggacg gtcggccgtt tgcgttttca acctcacgcc gcggacgcag ctcgcacaca
      481 tgaatcgcct caggaaccct tctgaaattg agacggccgc gcgccgcatc gccgctctgc
      541 tcgacgcccg cggcgagtgg ttctgcgcgg aggggcgcgg ggcggagcgg ccctccgcgt
      601 tgcgtcgggg cgagtgggag gtgagcgcgc agaggggcgc gttgctgctg tcgtactggg
      661 gcgcgggcgg agcgcgcgtg tggcgcgtgc ggggttggga gtggcgcggg ggaaagcttc
      721 tgctggaggc gacgcggcgg gccggcgccg tgcgcgcgac gttggagctt gtgccgcgcg
      781 cgtcggccgc ggccgacgcg gcggagttgg ccgcggcgag gcgcgcggag tgtgagcggc
      841 tggccgcgct cctgtgtgag agcgtggggc cgggggcgcg catcgaggag gcgcggctga
      901 gcgcgggcgc gcggcgcggg gagccgggac gctatgcgcg catcctgttg cggcgcgggc
      961 gcgagacgca cgcggccacg gggccaattg tcgagttggg gtcggaggag gcgggcgcct
     1021 tcgtcgcgtc ggcgctgttg tggttcgcgc aacttgggtc tcggcgcgcg ggcgccccgc
     1081 gaggtctgct tctgttcggg ccgaggaagc tgtcggcggc ggccgctgaa ttagtctcgc
     1141 tgcttcgcgc ggagttgcgc ggcgcgttgg aagtttacga gctgggggga ggaagaaaga
     1201 cgtttgagcg cgtgcgcgag cctgacctcg aagagttgtt ggacgcgtcg ccgcgactgt
     1261 cggggccggc gcgtgcggat ttgactgaga cagcggcgcg catccgcggg ctggcgccgg
     1321 aggcgattga cgtggtgcgc gcgcgtcacg gcgagacgct gcgcttccga ggtctcgcat
     1381 tcgcgcgcgt gcgccgactg cccggcgggg agagagcgtg gttcggcgtc gagggcgtgc
     1441 ggcagagacg ctcgctcgac gcgtcgagct ggcccgatct gccgcgcctc gtcggcgaac
     1501 tcgaagagca ccggcgagcc ggggggagcg ccggccgcca cgcactctac cgcgccgcgc
     1561 ccgaagcctg gctcgaatcg ctcctgcgcc gcgacgtgac ccggctcgac cccggcctcg
     1621 tcgtctcacc cgtccacgcg cagttccgca ccacgcgcgg ccagagggca cggggcggcc
     1681 ccatcgacct gctcgcactc cgccgtgacg gccgcctcgt cgtcatcgag ctgaaagtct
     1741 ccgaggacgc cgcgctgccc ttgcagggcg cgcagtactg gcggcgaatc tctacccacc
     1801 accgcgccgg ccacatcacg cgagcacgtc tgttcggcaa cgccgagatt tccgacgacc
     1861 cgccgctcgt ctatctcgtc gcgccgctcc tgcgcttcca ccgctcgttc cagacccttg
     1921 cccgcgccgt cacgccggag atcgagatgt accgcttcga cctcaatgaa gattggcgcg
     1981 ctggcgtgcg cgtggctcgg cgcgcccgta tcggttgagc agggttagag aatgaatggc
     2041 ggcctctact gcgaaacttt tgccggagaa ctaatcgcga tgagatgttg acccggcggc
     2101 tttgttgtat gctgatgcct gcaacacttc atggccgcaa aatattccga cgaggcgtgg
     2161 gcaacttcgt ttgctcgcgg atcgacgtaa caaactgagg ggggactcaa tgggtaaagg
     2221 caaacagaaa aatgacgaca agggtgtcaa cggcaaggtc agtgatctgg ctaaaaatac
     2281 tgaagacggc acggacgaat acctgacgac caatcaggga ttacgcatca acgataatca
     2341 gaactcgctc aaggcgggcg agcgcggcgc gaccctgctg gaagatttca tcctccgtga
     2401 gaagatcacc cacttcgatc acgagcgcat ccccgagcgc atcgttcacg cacgcggggc
     2461 ggcggcgcat ggtgtctttc aagtgtacga atcgatggcc gagtacacga aagccggatt
     2521 tctccagaac ccgatagtta agacgccggt cttcgtgcgc ttctcgaccg tcgccggctc
     2581 gcgcggctcg accgatctgg cgcgcgacgt gcgcggcttc gccgtcaagt tctacacgga
     2641 agagggagtc tacgatctcg tcggcaacaa tatccccgtc ttcttcattc aggacgcgat
     2701 caagtttccc gacctcgtcc acgccgtcaa gccggagccg cacaacgaaa ttccgcaggc
     2761 ggcctcggcg cacgacacct tctgggattt catttcattg atgccggaat ccatgcacat
     2821 gattatgtgg gcgatgtcgg atcgcgccat ccctcgcagc tttcgcatga tggagggctt
     2881 cggcgtccac accttccgtt tcatcaacgc caaaggcaaa gcgcgtttcg tcaagtttca
     2941 ctggaagcct ttgctcggcg tccactcggt cgtctgggac gaggcgaaga agatttccgg
     3001 taacgacccc gatttccacc ggcgcgatct ctgggaagcg attgagaacg gcgactaccc
     3061 cgagtgggaa ttcggcgtgc aaatcgtcga ggagaaagac gagcacaagt tcgacttcga
     3121 tctgctcgac ccgacgaaga tcatccccga agaactcgtg cccgtccggc gtatcggcaa
     3181 gttgaccctg aaccgcaacc ccgacaattt tttcgccgag actgaacagg tcgcgtttca
     3241 tccgggccac atcgtgcccg gcatcgattt caccaacgac ccgctcttgc agggccgcct
     3301 gttttcttac acggacacgc agctcaagcg tctgggcagc ccgaactttc acgagatacc
     3361 gatcaaccgc tccgtcgcgc ccgtccacaa caatcaacgc gacgcgcaca tgcgccagac
     3421 gattaacgtg gggcgggtcg cttacgagcc gaacacgctc ggcggcggtt gcccgtttca
     3481 ggcgggggcc gacgcgggcg gctttacgag ctacgccgaa aagatcgacg cccgaaaggt
     3541 gcgcgcccgc agcgagagct tcttcgatca cttcagtcag gcgacgctct tttacaacag
     3601 ccagtccgca ccggagcagg agcacatcgt caatgctttg cgcttcgagc tgggcaaagt
     3661 ggagacgccc gcgattagag agcggatggt ttacgtgctc acgcacatcg ataaaactct
     3721 ggcgggccgc gtcgcggaag ggctgggcat taaagtccct gtgaggattg acacgccttt
     3781 gaacatgagc gttccggcgg acggcgaccc gaagaagttt cagccgaagc gcgaggtcaa
     3841 agaggtcggc agctcgcccg cgctgagcat ggcgaacacc gtgaaggaca ctgtcaagac
     3901 acgtaaagta gccttcctcg ccgccgacgg gttcgacgac gacgccctcg ccgcgatgaa
     3961 gaaagctctg gcgggcgcgg gagcgcaggt gaagatcgtc gcgccgcggc tcggattttt
     4021 gaaagggtct ggcggcgctg aagtcaaaat tgatttcagt tttctcacct gcgcctcagt
     4081 cctgttcgac gcggtttata ttgccggagg cgaaaaaagc gcggcggccc tcaagggaga
     4141 agtcgacgcc atccacttcg tgaacgaggc tttcaaacat tgcaaggcga ttgccgcgac
     4201 cggcgcgggc gtcgaggtgt tgcgcgcctc atctatcggg gcgggcaaag cggattccgg
     4261 cggcggcaag gtcgtctcgg atgagggggt cgtcacggga caggatacgc aggcggggaa
     4321 agccgcggcg gagtttatca aagccatcgc ccaacatcgg cactggagtc gggaagcgaa
     4381 agctcaagta ccggcttaga cagcaatggt tgtctaagaa tatgacttgc tgatggagtt
     4441 gaacgccgcc gcgccgggga agcactgtcc cggcgcggcg gcgtttcgtt tatcaaaact
     4501 ttgagcggct gggaagggaa gtgctctcgc ggggcgactc ggtttgcgcc ggctgattcg
     4561 tcattgttgg gggcgggagt tttgcgcgag acagtcggcg cggccgtcgg ggccgcagcg
     4621 gaagagttcg ggcgcgtact tcatcgtgta ttcgacgacg ttcaagtaga cctgcgagtc
     4681 cgtgtacttg tcgtaggtgt agccgagccg cgacttgtcg cgccagacgc tacggaaggc
     4741 ggcctcttcg ccggagccgg cgagatgacg cgcttcgtgc aggaggatga cggcgcgctc
     4801 ggtgtcgtcc ttgggcctgg tgaagaactc ggggtagagc gtgacgacct cgaaggggaa
     4861 gttggtggcg gcgtaggcgt cggcgtggcc gaggtagttg ttccaccagt tgtcgctcgt
     4921 gcggaagttg gcgaggcggc gcaggaggaa ggcgtcttga tggaagccct tgcgctcgat
     4981 gagatcgacg gcgcgctgga cgacctgttt ctgttcgtag gtgacggcgg tcgaggtctc
     5041 cagcagcgag acgtagaaga ccacgagcaa caggagggcc gtcacacagc cggccagcac
     5101 tttccgcatg aggctgcgac gcgggcgggc ttcctcctcg aactccgcga agtcggccgc
     5161 cgccgactcg ccctgcccaa ggcgggcgaa ctcgtccgcg gcgcgtggcg cgaactccgc
     5221 cgagagaagc gcgccgcagc gtttgcattc gtgcgccgtc gaaaagttga cgaggccgca
     5281 ggaggggcat ttggagttca tcgtaaatcc tcgcgagatg aattgcggca aaggagaggc
     5341 ttgattcatc ggctcaagca aaacagtacc gcgagcggaa gcttcgcggg tgtgctatcg
     5401 atcagaggcc cgcccggcgc atccagaccc gcgaggcttc cgctcgcggt actgtgcgtg
     5461 gcggcgaccg gccttctatt agtcgtccgc cgcgtcgcgg acgtaggtgc tgggggtcgt
     5521 gacgcgctcc atgtcttcgc gggcctgatc ggaggccgag cgaatgccgg cgatgcggag
     5581 gcggaactcg tcggcgttcg acgccccgcg cagggcttct tccaagggaa tgaggcccga
     5641 ctggtagagg ttgaagagcg actggtcgaa ggtctgcatg ccgtactgcg aggtgccggc
     5701 ggcgatggcg tcgcggatga gggaggtctt ctcctcgttg acgatgtagt cgcgaatcag
     5761 ccccgtcgag accatcactt cgaccgccgg gacgcgcccc ccgccgcgcg ccgcggggat
     5821 gaggcgcatg gagatgaccg ccttcaggat gccggcgagc tggatgcgga cgctcttctg
     5881 gaggtgtggc gggaaggtgg agacgatgcg gcggatggtc tcggtggcgt cgagcgtgtg
     5941 gagggtggaa agcacaaggt ggccggtctc ggcggccgac agggccgtct cgatggtctc
     6001 gtagtcgcgc atctcgccga cgaggatcac gtccgggtct tgtcggagcg cgccgcgcag
     6061 ggcctcggag aaggagcgcg tgtccacgtc cacctcgcgc tgggtgacga aggaacgctt
     6121 gtcgcggtga aggaactcaa tcgggtcttc gatggtgacg atgtggccgc cgcgcgtgga
     6181 attgatgtgg tcgatcatgg cggcgagcgt ggtggacttg cccgaccccg tagtgcccgt
     6241 cacgaggatg aggccgcgct gctcgtcgcc gatgcgctcg atgacgggcg gcaggctcag
     6301 ctccgtcatc gaacgcatgc ggtcgggaat cacccggagc accatgccga cggtgccgcg
     6361 ctgctggaag atgttgacgc ggaagcggcc gaggcccgtg acaccgtagg cgatgtccac
     6421 ctcggacgcc tctttgaagt gctgcttctg ccggttcgac atgatggaga aggccatgtc
     6481 gagcgtgtct tcctgcgtga ggcgcggcgt ctcggcgagg gcccggagtt cgccgctgac
     6541 gcgcatgaaa ggctgcgagc cggccttgac gtggaggtcg gaggcgccgc tttgcgtggc
     6601 ggtcttgagc agatcgttga ttttgatgct catagcttaa ggatttgtgc cgggacgaaa
     6661 aggagtcggg cggatgaagg atgagaactt ggagaggaag cgcggacggg gctaagtggg
     6721 gccggcccaa aagttacgac cggacggacg ggccggcgta gaatcattct agcccgtgcc
     6781 cggcgaggcc gtcaacgata ttacttgagg agaggccaaa gcgtcggcgc gcaacccttg
     6841 cccgccggac gcctcccgcc tctaaacgcc ttcgtccgtt gtgcggctcg gcccggtgct
     6901 actcggtcat gaccgactcg aaggtgacgg gttcagccgc cttctcagcc gggcgcggct
     6961 gttcggggtg gaagccgttg ccggcctcgg acgccgccgc cgccttgtgc tcgttgacga
     7021 aagtgagcca cggccccggc gcggggcgct cgcccttcgt cacctcgaag taggcctttt
     7081 gcaactgcgc cgtgatgggg ccgcgccggc cctcgccgat cttgatctta tcgacggagc
     7141 ggatgggcgt gacctcggcg gccgtgcccg tgaagaacaa ctcgtcggcg atgtagagcg
     7201 cggcgcgctg gaggcccgac tcgacgacgg ggatttccag ctcgcggcaa atctggatga
     7261 ccgagtcgcg cgtgatgccg ggcaggatgg aagacgagag cggcggcgta atcaccttgc
     7321 cgttggagac gaggaagatg ttttcacccg acccttcgga gacgaagccg cggtcgtcga
     7381 gcgcgatgcc ctcggcgtag ccgttcttga tggcctccat cttgatgagt tgcgagttca
     7441 tgtagttggc gcccgacttc gccatcacgg gcatcgagtt cggtgacagc cggttccacg
     7501 aggagacaca cacgtccacg ccctcctcga tggcctcctc accgagatac ttgccccagt
     7561 cccacgcgcc gatgtagcag gagagcgggt tggggaaggg gttgacgccg aaggccgggc
     7621 gctcctcgtc gagactgcgg aagatgatcg ggcgcacgta gcactgctcg aagccgctgc
     7681 gcgcgacgag tccgacggcc gcctcgcaca gctcgtccac cgaccactcg tgatccatcc
     7741 ggtaaatctt cgacgagttg acgagccgct gcatgtgctc gcgcaggcgg aagatggcgg
     7801 ggccgcgccc cgtcgggtaa cagcggatgc cctcgaagac gctcgaaccg tagtggagga
     7861 cgtgcgacat gacgtggacg cgcgcgtcgt cccacttgat gaactgtccg ttgtgccaga
     7921 tttccgggac aatgcgcgcc gtcttcacgt ccttcttcgg cccgtcgtca ttaaacatgg
     7981 cttggttaca ctccttcccg gattaaaggt ttaatcagcg gggaattgcc tgagataatt
     8041 gcggcgagga cggcttactt cgtcgaggca gaacaacatc actcgcctga attttcaccc
     8101 ggggttgtat gaccctgaac gataccgtat agccgcgccg cttggcaagg cactcaaaag
     8161 cagggatgaa ggatgaggga tgaaggatga agaagaattg attcatcggc tcattgactc
     8221 attgattcat tggccttaag tttttaattg caccgacgaa cgaatgagtc aatgaatcaa
     8281 tgcgccaagt cttattcatc cttcatccct catccctcat ccttgtcttc gccctcgccg
     8341 gctgccggca acaggcgacg acggacaaca cgaacatgcc cgacccgaca ccacaaacga
     8401 atgcgaccgc caccgccgcc gacaaggccg ctgacaagga aagcgacgcc ctctccatcc
     8461 accgccgcgc cctctccatc gacatgcacg ccgacaccgt gcagttcatc ctcgacgagg
     8521 gcgccgacat cagcgcaagc gtgcccggcc tccacctcga cgccccgcgg atgcgcgccg
     8581 gcgggctcga cgcgcagttc ttctccatct ggatcgagcc gtcgtactac ggcgcgggcg
     8641 gccaactcgc ggtcgagcgc gccgacgagc aggtcgcggc cgtccgcgcg ctcgccgaag
     8701 cgcgccccga cgtgtgggcg ctggcgacga ccgccgacga agttcgacgc gccgcaggcg
     8761 agggccgact cgccgcgctc atggggcttg aaggcggcta cgccctcgac gagcgtctgg
     8821 agaacgtcga gaaatattac cgcgcgggcg tgcgctacct gtcgccgacg tggagcgtaa
     8881 gcctcgcttg ggccggctcg tcgggcgacg ccgcggggca gtcgcgcggc ctgtcggact
     8941 tcggccgccg ggtcatcggc gagatgaacc gcctcggcat catggtcgat gtctcgcacg
     9001 tctccgacaa gaccttctgg gacatcatcg acgcttcgtc caagccggtc atcgccacgc
     9061 actcgaacgc gcgggccatc gcgcccgtgg cgcgcaatct ctctgacgac atgatccgcg
     9121 ccatcgccgg aaagggcggc gtcgtgtgcg tcgtcttcta cccgcagttc atcgagccgg
     9181 gttgggagga gaagaaactg cgagttgacg ccgagatcgc cgggatggtc ggcgcggcga
     9241 gcgaaagggc cgtgggccgt ggttcagccc ggcgcatcgc gcgcgaccgc gtgcgcgaac
     9301 gcgagtacgc gcgccggctc ccgcccgttt cggcggcgcg cgtcgtcgat cacatcgacc
     9361 acatcgtccg cctcgtcggc gtcgcacacg tcggcgtcgg ctctgacttc gacggcattc
     9421 aggccgtccc ctccgacctc tcttcggtcg ccgacctgcc gaacctcacg gccgaactcc
     9481 tccggcgcgg ctacaagaaa gaagacattc acaagattct cggcggcaac atcctgcgcg
     9541 tgatggcgga ggtcgaaaag cattgagcgg gcggcgtcgt gaaatgtttc ggctcagact
     9601 cgcataacag atgcaggcgc cgggagagtt gtccgattct gacccgtcgt tcaaaggcga
     9661 acacgacctg ccactcatcg tcaggttcgc ctaaccgaac ggccatcctc ttgctggcct
     9721 gccgccacgc accccgtgag gagaagaacc cgcgcggggc gcccgcacga aaccacctcg
     9781 tcaaccttac tcgggaataa ctgaacagga gcgattcaca tggctaatgg aactgtggtc
     9841 atagaccccg gacacggagg cagcgtcgag atcggaggct cgtcagccaa caacgccatc
     9901 agcgccagcg gcgtgctcga aaagaacatc actctgcgca tggccttcct ggtgcgcgac
     9961 gccttgaagg aactggcgac actcggaggc cacaccctcc agattcacct cacgcgtgat
    10021 accgacaaga acctgggtct ctgcgaccgg gccaaagtcg cgaagacgaa aagcgcgaac
    10081 atcttcctga gcatccactg taacggcttc gacaaaacgg ttcgcggcac ggagacgctg
    10141 atccggctcg cggccagcaa cccgaagcac gccgccgaca aggccctcgc gcagagggtg
    10201 cagaaggctg tcttcaacgc gatcaaatcc cacgacgcga acgccaaaaa ccgtggggtc
    10261 aaagatcagt tgctcggcgt cttgaaccgg aactgcctcg gctccaagcc ccgcgggtgc
    10321 atggtcgaga tggagttcat cgacgtgccg gccgttgaca agctcctgaa catcgggccg
    10381 aactccccgc aggtgcgcag cgacatcgcc aaagccatcg ccggcgcgat catcgacgac
    10441 ctgattgcca atccgtagag gcgccgaccg gcacgccggc aaaaacgtgg gccgcgacgg
    10501 gcgtcgtctt cgggcacgcc cggttgattt tagaagcgcg ccgggtgatg attcgccaca
    10561 tgaaatgccc cgccctcttc gcgcgccggg ggacgatgtg gctggccttg tccgtcgcct
    10621 gcctgtcgcc cctcgcgctg gccggttgct cgcggagtca gccggcggcg tcgaactctc
    10681 cggccgccgc acatcagacc ccggcggcca cgccgtcgcg cgcctacact ccggtcttcg
    10741 cgaggcgcga ggcggcggcc gccgggcaga agcgcgggac gctcggggac gacttccagt
    10801 gggccgccgg cgcggacact ctggaggagg ccgcggcgcg ctgggaagac tttctgaaga
    10861 ctcacgcgcc cgcgggcgcg gcgggcgcgg cgggcgaaga gtacgaagac gcctttcagg
    10921 cgaaccacgt ctcctcggcc cggtatgagt tgatgcgcgt ctattatctg ctcggccggg
    10981 ccggggacgg cgaccgcctt ctgcgcgagc tagacccgct gtctttgtcg aactgaaacg
    11041 ccgccggcgc ggccgcgccc ctcacccccc atcccgaccc cggcatgacc ccacgcccac
    11101 gcccatcttt gtgaaaagca acggtgctgt tggataatgt cggccatgaa cattgagctg
    11161 aaggaacgct ccgaggggcc ttactatctg caagtgcgga cgcagatcga gacgctcatc
    11221 cgcgagcaga aggtcgcggc cggcgacgca ctcccctcgc cggcgcagct cgcgcagcag
    11281 ctcgccatcg accgcggcga ggtgcaacgc gcttacttcg agctggagca ggccggcctc
    11341 atctccaaaa aggtcggccg cgattttctc ggcaaggaga aggtgacata cgcggtgaag
    11401 tgaaggggcc acgcgcggtt tcggtcgcgt cgcttacgag cgaaagttcg acaagcccgc
    11461 gcgacttgcg ttaacatctt cagcatcttt tgaagtgaag gacgttttgg tcagcacatg
    11521 aaacgctttc agcatcccgt caagctcctc cttacggtgc cgccccgcgg cgaccgcgcg
    11581 tgagtccact cgcgcgcaca ggtcgcggcg gcgcggccga ctccaacgcg acagcaaaaa
    11641 tcccgaaaat caaaactctc gcgcgccgcg tcgcccgccg gacttcagcg cgtcgtcgtg
    11701 ttcggccgtg aggtgaagga aaggcttcgg tgacaattat ggacgagaaa tatttcgcgg
    11761 aagtggtcga ggagaagtgg caacgccact ggaaagagtc gggcgcgttc gaggtcgagc
    11821 gcgacgacgc gcgtgcgcgt ttctactgtc tggagatgct gccctacccg tcgggctatt
    11881 tgcacatggg gcacgtccgt aactactcca tcggcgacgc gctctcttgg tacaagcgtc
    11941 tgcgcggctt taacgtcctg caccccatcg gctgggacag cttcggccag ccggccgagc
    12001 aggcggccat caagcgcggc gtcaacccga aggactggac ggaggaaaac atcgcgcaca
    12061 tgcgcgggca gttgcagcgt ctcggcgtca gctacgactg gcggcgtgag atcgccgcgc
    12121 acacgcccga gtactacaag tgggatcagt ggttcttcct caagatgctg gagcgggagc
    12181 tagcctacaa gcgcctgtcg cccgtcaact ggtgtccgac ttgtcagacg acgctctcga
    12241 acgagcaatc gtcgggcggc gtctgctggc gctgcggcaa ccccgtggag cggcgcgaca
    12301 tcgagcagtg gttcttgcgt atcacggcct acgccgaggc gcttttggaa gacatgaagg
    12361 agatcgaggg cgcgtggccc gagcgcgtgc tgacgatgca gcgcaactgg atcggcaaga
    12421 gcaccggcgc gttcgtcgat ttccagatta aggacggcga ggacaagatt cgcatcttca
    12481 cgacgcgcgt cgatacgatc tacggcgcga cggcggtcgt cgtggcggcc gagcaccccg
    12541 tagtcgagcg gatgctcgcg gaaggcgggc cgtcggcgtt gaaagacgag gtgcgcgctt
    12601 tcgccgagcg cgtcagggcg acgcacggca cgtccgtgaa ggtcgcgggc gaggagcagg
    12661 aggagaagga gggcatcaat acggggttga tcgccatcaa cccgttcagc ggcgagcagg
    12721 cgcccgtgtg ggtggccaac tacgtgctga tggagtacgg gacgggcgcg gtgatgtctg
    12781 tgccggcgca cgacgagcgc gactacgagt tcgcgcagaa gtatagcttg cccatccgac
    12841 gcgtcatcga gcgcatctcg cacgagcagc ccgtcatcga gcacgacccc gaccacgaag
    12901 atcagtcggt ggcgatgcat caggcgttta cggaatacgg catcctcgtc aactccgccg
    12961 actggagcgg caagctctcg gaagacgcca cccgcgagat ggccgagttc gcgcgtgaga
    13021 gcggcttcgg cgagccggcc acgaccttcc gcctgcgcga ctggggcatc tcgcgccaac
    13081 gcttctgggg cgcgcccatc cccatcatct actgcgagcg gtgtggcgcc gtgcccgtgc
    13141 cctacgaaga cctgcccgtg cgtctgccgg agcgtgtgga gttcggggcc acggggcagt
    13201 cgccgctggc gggcgtcgag gagttcgtta atgtcagttg tccgaagtgc aaaggtgcgg
    13261 cacggcgcga gacagacacg atggacacgt tcgtcgattc ctcgtggtac ttctttcgct
    13321 actgcgaccc gcgcaacacg gaagcgccgt tcgactctga gacggcccgc tactggacgc
    13381 cggtcgatca atacatcggc ggcatcgacc acgcggtgat gcatctgctc tacacacgct
    13441 tctggacgaa ggcgatgcgc gacatggggc tggtgaattt cggcgagccg gtgcggcggc
    13501 tgatgacgca gggcatggtg acgaatctca tcggcgagac gggcgagtgg aagcgcatgt
    13561 cgaagtcgcg cggcaacggc gttgaccccg acgacatgat tcaggcatac ggttccgacg
    13621 ccgtccgcct gttcatcctc ttcgccgcgc cgcccgagag cgagctgcgc tggcaggaga
    13681 ccggcatcga cggcgcggtc cgctttctgc ggcgcgtcta ctcgatggtc taccgctggc
    13741 gcgacgcttt gagggacgcg cccgtcgagg ctccgtccgc tgacgacttc acaacgccag
    13801 ctcggaagtt gcgccacgag acacaccgca caatcgcgcg cgtcaccgac gacttcgagc
    13861 gactgcatct gaacaccaac gtggccgcgt tgatggagct gtcgaacgcg ctcggcgatt
    13921 ttcagacgaa gccggagggg gcgacggcgg ccgagttgtt cgccgcgcgt gaggcgttgg
    13981 aggcgctggt gctgatgctt gcgccgtttg cgccgcacgc cgccgaggag atgtgggagg
    14041 gactcgggca cgagggcggc ctgctgggcg gaggccgcgc gctctggccg tcggccgacc
    14101 cggcgctcgc gcgcaaggag gagctggaga ttcccgtgca ggtcaacggg aagttgcgcg
    14161 ggcgtctgac cgtgggcgcc gacgcctcgg aggatgagct gcgctccgcg gcgctcgccg
    14221 acgagcgcgt gcgcgcgtgg gtggaaggga aggagatcgt taaagtggtc gtcgtcccga
    14281 agcgtctggt caacatcgtc gtgagataat acggcggggc gggaaccgtg agcgcccgcc
    14341 cctcgttcta cattgcgacc cctgattttt cgcgccgcgc gcgttataga tcatttcaac
    14401 ttgagtgtta ggtatggcgg ctcaccgcgg aggcacagag aacgcagaga ctacgccgca
    14461 ggcttgacgc ttccgctcga cgttgaatct gctcaaccct ctgcgcttcc tctgcgtctt
    14521 ctgcgcctcc gcggtgagtc caacgccccc aagactcgac tgaaacaagg gtaaaatctt
    14581 ttcgaggtcg cccgcatgaa gttcaaagac tctcgcatca tcgccgtcat cgtcgccctc
    14641 attgcgctcc tccacttcgc gctctccccg cgcgaagtca agcaagtctc gcccgcccgg
    14701 actcaaacca cggagtcgtc gagggccgcg cgccgcgcca tcgtcatctc gcttgacggg
    14761 ctgga
//
LOCUS       DASVES010000126        16081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1000199, whole genome shotgun sequence.
ACCESSION   DASVES010000126 DASVES010000000
VERSION     DASVES010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16081
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000199"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1276
                     /locus_tag="VF240_04550"
     CDS             332..1276
                     /locus_tag="VF240_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7174538.1"
                     /translation="MKTAIIHHPVYEKHDTGDGHPETAARYAAVMRALRDDQQLWPRL
                     LELEAPAAPRGDVQACHTPQHFKRVERAVSEGLGYLDADTIVSMRSLEAALRASGAGL
                     RAVDAVMKGEARNAFLPVRPPGHHATSERAMGFCLFNTVAVAARYAQNRYAEVERVAV
                     VDWDVHHGNGTQGIFYDDPSVFFFSAHQYPWYPGTGARGETGVGRGRGTSLNVPLKAG
                     TPAAEHRRAFDAALSEIGAKFKPDLVIISAGFDAHTSDPLGQLTLEDDDFVAMTRTVK
                     QWAADACGGRVVSCLEGGYNLQTLGATALAHVRALAEE"
     gene            1509..2906
                     /locus_tag="VF240_04555"
     CDS             1509..2906
                     /locus_tag="VF240_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7174539.1"
                     /translation="MASLTYKQLISGNRSFRRLLAGQVVSELGNWFNFIAGLGLVRAV
                     TGAAPEPLAILVVLRLAPFALFAPLAGAVADRLSRRTVMIVTDVLRALLALGFLLVRG
                     PEDLWIAYVCTFGSTVLAAFFEGAKNAALANVVGGRGLLAGNALMFSSRFLLMSVGSA
                     LGGEASAAFGYDVAFVINALSFLVSAYSIWLIPGREMHAAEAVSESGDEDARGTNEIG
                     TPAGSDVRAGAGKRAGYLADVLEGWRYVVRHPLVAAIFGINIMWALGGGGLNLVYERL
                     GAVVFAEAGGLQPDRAVSMLYTAAGAGLFVGMFLARRVGAKIEVRGATARFMGWTLIA
                     HGILFALSGLMPSIWWCMLMMFLSRAVIGVEFAIQETLLLRLLPDRLRGRVTSSDRAA
                     EILMMSLTTAAAGWLLGFALTPRSLTVVSGLLCGSPGIVWLLLFASGKLRMPERPAGA
                     DEDDEEGETALASAN"
     gene            3160..5511
                     /locus_tag="VF240_04560"
     CDS             3160..5511
                     /locus_tag="VF240_04560"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7174540.1"
                     /translation="MKRFDGATAKTLEFAARVRAALRRTMPRVALVAAGLLVLWVILG
                     NTSISETRVDNFFDRLTFLAVASVALYYCVLALHWTKRKLLWRVRRRLLITYLFVGLT
                     PLVLLGVLGILAWLVLSGQAMSRIVNVQINATEQVAQANADALAAEFLRRPIGASASE
                     NQRWLDERMAVLQAGLPGARAAVWRGGAGQRANTEGEELGVVGEGRAADFMSAAHGGE
                     EARGIGVERVSLDAPLPAWLRGQERWSGFAFVPPPDESADPFGTPSVRALVRRGEGER
                     GVAVLLVVPVSRALVRQFRESTGIALRPYFLGARKLDVNVATKSDGISINSRREPDGE
                     RQKGLELNPDAQLAAIDNAPGDRDQFGDQLPNGPPYIVALPATDWLNGEVDTHLSFLF
                     EYSLAEARRQLLDSSAVGEVIQKALVALAIVFLALELVALLAAAWMTRAVTGTVHKLH
                     LATQFIKRGDFSHRIRTRSHDQLGELAAAFNDMSANIEVLLEERVERERLEREVEIAA
                     EVQAQLFPRSLPALTTAEVTGECRAARGVAGDYYDYVEVEPGLVAFVLGDVSGKGISA
                     ALVMSNLQASLRAQVTIISERRRLVGLAAATTTAASAAAASDGGEGLGACGVTGADSA
                     CAVENMVSSINQQLCRSTDSNRFATLFLALYDDRARTLRYTNAGHNTPILVRANGRVE
                     RLDVGGTIVGVFDFVTYEEAQARLDLDDLLVVFSDGISEAQNAAGEEYGEERIVQLAA
                     ANRHLAADDLRHKIFDEVDKWSGDEERGDDQTLVILKAVISDE"
     gene            complement(5573..6505)
                     /locus_tag="VF240_04565"
     CDS             complement(5573..6505)
                     /locus_tag="VF240_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174541.1"
                     /translation="MNATHHFEQTPDGWLGFELSVLRRLKIRSLANPFAGEADLDFAL
                     KRWGVRVSTNDVWRWSWSKASARVENNSERLSVEDVALVLEDAYVPHYRLANPALRKW
                     FGETDAWWFDNVRRNAEQLASPTRRHLALHLGVMVGDYVLSFDDESRELRQPLSRVFQ
                     RLWESEPRPVDNRESNKAANKEPRDFLSTEQADCLFLRLPRPARRGARHTAWAWREEW
                     IRGGDGFWDEFEGARAGRLGARAETRQQYLRHVEELLAAAAHIPAWAVAFTEDGLINT
                     DEMVETIRRARKVGTVYTKDFAELVGARAVMVTA"
     gene            complement(6543..7349)
                     /locus_tag="VF240_04570"
     CDS             complement(6543..7349)
                     /locus_tag="VF240_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7174542.1"
                     /translation="MSTELVENDRTTGEAFRRLAASVDAFEGYEHPHASRVARLSDEL
                     ARHFGLSRADRTSLVHAALLHDLGEMAMKRDYISRAGSLKEEERLDLARHPVIGEQES
                     GRAGAERAVQLLVRWHHEWWNGAGYPDCLRAEQIPLKARILRVADSYAALTDSRPWRS
                     AGTEAEARKHLTLWAGLEFDPAVVRAFLSLRDIPELRSYARAAEPQSATPPPAPEPQT
                     PPATDAQSVEESAVTDEADQREEVAGEAPPEPVEASFGQAPPELEKSDSR"
     gene            complement(7512..9062)
                     /locus_tag="VF240_04575"
     CDS             complement(7512..9062)
                     /locus_tag="VF240_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7174543.1"
                     /translation="MKGQLREHPLVELVREINAAGLSGVLRLARERVKAVVYFNAGEA
                     VFAATNLRAHRLTFLLRQWGAVAPERLLEAGVDALSDELAGAALVSSGAVSAEELAAL
                     RLRQSGEALRPPLLWTEGEWAFDPRARLQGVAHAPLPAGQMLLEAARRLPAEFVAARF
                     PDRGESVSRPGQLSADLPLQPEEGFVISRLDAPLRVSELAAMTGLPEEQTLRAVYTLF
                     LYGLLRRERAPVALDAAQVAQALAAQAVEPKESAPPVSVEQPREEPAAEEETDPLAAL
                     DTLFARAAGHTHYEVLGVGRSVPPAEIKRAYYALARRFHPDRFSREADAELRARIESA
                     FARIAKAYDVLSDPKARAAYDLRLGRTPDASGGQRQQQREQRPDASAGGGDGVSGKTA
                     GAGDVPPGAEEKFRQGQEALRQNDHEAAARLLGEAAMLAPQQARYRAYHGRALAHNRR
                     MRRLAETELQAAVALDPRAADYRVMLAELYRDIGLRRRAEEELERALTLNPTHAEARR
                     LLRSLRES"
     gene            9344..10180
                     /locus_tag="VF240_04580"
     CDS             9344..10180
                     /locus_tag="VF240_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7174544.1"
                     /translation="MASVEGAGGARGGELESVIERLVEELRERTAELESANGELHRIG
                     RYRSLFLARMSHELRTPLTSILGFAEILLDQEDLDERQRRFCQKIQDSGMRLHASLNQ
                     LVDLSRLEAGQTELFLHEFSLRETLRESCAAVARLAEKQEVNLDCHTPPDLSNVVSDE
                     GKLRQVLYNFLAHAIGRSPAGESVRVEVTVADAGGLAIAIRDEGAPLEDPEHLFEPVD
                     VDTPNERGTNMNELGLVIAHRLIDALGGTVSLSHPGGHGLEVRLSLPESPRERAELAR
                     PA"
     gene            10282..11082
                     /locus_tag="VF240_04585"
     CDS             10282..11082
                     /locus_tag="VF240_04585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174545.1"
                     /translation="MTSLPETTERSASGVEADALTIRECVSVEELDACVRLQREAFGL
                     PDLELSPRRHLVVARAAGGWVLGAFDGDALVGFVLHLAAARGAEVIGYSHMMAVAPGQ
                     QNRGVGARLKWAQRARSLAEGRRFIRWTWEPMRARNAHFNLNRLGVVVRAYAADFYGA
                     DYAATPEEQGARRGLASDRLSAEWELRSPRVEALARGQQPAPPGAPAAAVEIPPDWNA
                     LVRQDPAAAQRALLRTRDEFQRAFAAGLVCAGFERHDAQPRYLFYSDK"
     gene            11155..12570
                     /locus_tag="VF240_04590"
     CDS             11155..12570
                     /locus_tag="VF240_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7174546.1"
                     /translation="MPFTRYDRYVDRHARSFVERLQALCRMPSVAARGTGMRAVAETV
                     EQMLQRAGAGTRTFKIGSGFPIIYGECGAGPRCFIVYGHYDVQPIGQLSEWSSGPFAA
                     SVVGDKLYARGASNSKGDLVARLAAVEAYQKTYGKLPVCLRFLIEGEDGLASPSLYRF
                     VEDHAEMLRADGCVWDDGAKDTKERLVINLGFKGISFLELRAYGARTDLHSKWGGIVP
                     NPAWRIVQALATVVSPKGVITIDGFSHHVAPLTAEDEKTLKTVELDEPGLKREFRIGG
                     WVRGLTGKHLARELIFGPTATICGIHTGHTEAGAKTVLPGTAVARLDFRLVPDLTPDL
                     VVKLLRKHLDARGFQDVEIIELGAAPTAKSSPRSFVARAAVESVAEIYGAPPVIFPLD
                     PASGPVGAVCGVQQPATPIVSFGISYAGSNPHAPDENIRLDDFLRGIKYFGRIIHRLA
                     HDEDNDEDMNAQREAPVQISK"
     gene            12928..14946
                     /gene="ligA"
                     /locus_tag="VF240_04595"
     CDS             12928..14946
                     /gene="ligA"
                     /locus_tag="VF240_04595"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7174547.1"
                     /translation="MSASAKSIEREVQRLRGEILRHEELYYVKDSPEISDAEYDALLE
                     SLRRLEEEHPELQAADSPTLRVGGRPAEGFEEYVHRRPMLSLENSYNIDELRAFDERC
                     RKLADGREFDYVAELKIDGLSLSVHYADGVLARGVTRGDGRRGEDVTQNVRTIRTIPL
                     RLKDGGGAGDVEVRGEAYLSRREFERINAEREEASEARFANPRNAAAGTIRQLDPKVV
                     SRRRLDLFAYDCIRGERKAFATHNESLEWLVRAGFHVNPDHRVCGTIEEVIAFCNEME
                     AKRDSLEYEIDGVVVKVNSTALQEEFGTTAKAPRWAIAYKYPARQATTEILDIKVQVG
                     RTGALTPVAILAPVQLAGTVVSRATLHNEDEIKRLDVRVGDLVLIEKSGEIIPKVLKV
                     LKTQGKRRKKAFELPDKCPVCGGVVSRPEGEVVSRCVAADCAAQLKARLLHFASRRAM
                     RIEGLGDALAEQLVQKRMMCDVGDLYKLPLEEVVALERMAEKSASNLMAQIEASKGRD
                     LPHLVYGLGIRHVGERTAAILARHFGSLERLSQATLEELDDIHEIGLTVAQSVRDWFD
                     DEGNRALCERLRQAGVRTEMEQGITGVAARDGAFAGKQFVLTGRLETLARDEARALIE
                     SAGGRVTSSVSKKTDYVVAGEEAGSKLDKARELGVNVIDEAALREILG"
     gene            15154..15597
                     /locus_tag="VF240_04600"
     CDS             15154..15597
                     /locus_tag="VF240_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174548.1"
                     /translation="MNKEEQSQNIELVYTLFCDDVRLEVGNKLSLMGVFHQIAVQQFP
                     VSLMKFAVVSQWRGEGTHLSEVRILTDDRRQPVVVAEPSRFEVMPGGVANNISFFFNV
                     VFHTPGRYWVQTLIDSNLTDEQPLTLLALDAQGMPRLDDASEAVN"
     gene            complement(15693..15776)
                     /locus_tag="VF240_04605"
     tRNA            complement(15693..15776)
                     /locus_tag="VF240_04605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15740..15742),aa:Leu,seq:cag)
BASE COUNT         2705 a         5274 c         5380 g         2722 t
ORIGIN      
        1 tgcccgaaca cgtcgggcac gagcgccgtc agcagaaaca ccgcgcagat gatgattgac
       61 aaaaactttt tcataacctt ctccctcgtc ctctagcctc gataaatgtt aatggagaga
      121 tgactcacgc ggtttgtcgg agcaaatacc attccgcgtg ggagtcggcc gtcattgggg
      181 tcaaaaggca aacaagtcag cgacttaacg ccggcggaag gtcgtctgtg gttgctcttt
      241 tgcgacacag cgagcgcgac ggcgtatgaa tttgaagcat cggggcggcc ctgctttgac
      301 ctgtctccgg cgcttccgca taatcggcac tatgaagacc gcaatcatac atcaccccgt
      361 ctacgagaag cacgacacgg gcgacggaca cccggaaacg gccgcgcgct acgcggccgt
      421 gatgcgggcg ctacgcgacg accagcagct ttggccgcgc ctgctcgaac tcgaagcgcc
      481 ggccgcgccg cgcggcgacg tacaggcgtg ccacacgccg cagcacttca agagggtcga
      541 gcgcgccgtg agcgaggggc tcggctacct cgacgccgac accatcgtgt cgatgcgttc
      601 gctggaggcg gcgctgcgcg cttcgggagc gggcctgcgc gccgtggacg ccgtgatgaa
      661 gggggaggcc agaaacgcct tcctgcccgt gcgcccgccc ggccaccacg cgacgagcga
      721 gcgcgcgatg ggcttctgtc ttttcaacac cgtggccgtc gccgcgcgct acgcgcagaa
      781 tcgctacgcg gaggtcgagc gcgtggccgt cgtcgattgg gacgtgcatc acggcaacgg
      841 cacgcagggc atcttctacg acgacccgtc ggtctttttc ttctcggcgc accagtaccc
      901 gtggtatccg ggcacgggcg cgcgcggcga aaccggcgtc ggccgcggcc gcggcacctc
      961 gctcaacgtc cctctgaaag cgggcacgcc ggccgccgag caccgacgcg ccttcgacgc
     1021 ggcgctctcc gagatcggcg cgaagttcaa gcccgacctc gtgatcatct ccgccggctt
     1081 cgacgcgcac acgtcagacc cactcgggca actcacgctc gaagacgacg acttcgtcgc
     1141 gatgacgcgg acggtcaagc agtgggcggc cgatgcctgc ggcgggcgcg tcgtctcctg
     1201 tctcgaaggc ggctacaacc tgcaaacgct cggcgcgacc gccctcgccc acgtccgcgc
     1261 gctcgccgaa gagtaagtcc gccgtctatc tcggcggaag aagaattaac atggatggac
     1321 aggacgcaca ggatttgcag gacggacagg acgagacgat ttgaaattct caaatctcac
     1381 atctgaaatt tcaaatcagc tctttcttta tcctgcccat cctgtcaatc ctgtctaaac
     1441 ctcctgcccc ttgagtagat ggcggacttg ccccgcccta caggctcgcc gctttttcat
     1501 ttcggctcat ggcgtcgctg acctacaagc aactcatctc gggcaaccgc tcgttcaggc
     1561 ggctgctggc ggggcaggtg gtttccgaac tcggcaactg gttcaacttc atcgccgggt
     1621 tgggactggt gcgcgccgtg acgggggcgg cgccggagcc actggcgata ctcgtcgtgt
     1681 tgcggctcgc gccgttcgcg ttgttcgcgc cgctggcggg agctgtcgcc gaccgcctgt
     1741 cgcggcggac ggtgatgatc gtcacagacg ttctgcgcgc cctgctcgcc ctcggcttcc
     1801 tgctggtgcg cggccccgaa gatttgtgga tcgcctacgt ctgcacgttc ggctcgacgg
     1861 tgctggcggc cttcttcgag ggcgcgaaga atgcggcgct ggccaacgtc gtcggcggac
     1921 gcggactgct ggcgggaaac gctttgatgt tctcgtcgcg gttcctcttg atgtcggtcg
     1981 gctcggcgct cggcggggag gcgtcggccg cgttcggcta cgacgtggcg ttcgtcatca
     2041 acgcgctgtc gttcctcgtc tcggcctact cgatctggct catccccggc cgcgagatgc
     2101 acgctgccga agcggtgagt gaatcaggcg acgaggacgc gcgcggcacg aatgaaatag
     2161 gcacgccggc ggggagcgac gtgcgcgccg gcgcgggcaa gcgcgcgggt tatctggcgg
     2221 acgtgctgga ggggtggcgc tatgtcgtcc ggcacccgct cgtggcggcc atcttcggca
     2281 tcaacatcat gtgggcgctc ggcggcggcg gcctcaatct cgtctacgag cgcctcggcg
     2341 cggtggtctt cgccgaagcg ggtgggttgc agccggacag ggccgtctcg atgctctaca
     2401 cggcggccgg tgcgggcctc ttcgtgggga tgtttctggc gcgccgggtc ggcgcgaaga
     2461 tcgaggtgcg tggggcgacg gcccgcttta tggggtggac gctgatcgcg cacggaattt
     2521 tgttcgcgct ctcggggctg atgccgtcga tttggtggtg catgttgatg atgtttctgt
     2581 cgcgcgcggt catcggcgtg gagttcgcca tacaggagac gctgttgctg cgtctgctgc
     2641 ccgaccgcct gcgcgggcgc gtcacttcgt ccgaccgcgc ggccgaaatt ctcatgatga
     2701 gtctgacgac ggcggccgcc ggctggctgc tgggtttcgc gctgacgccg cgctcgctca
     2761 ccgtcgtctc tgggctgctc tgcggtagcc ccggcatcgt ctggctcctg ctgttcgcgt
     2821 cgggtaagct gcggatgcct gagcgcccgg ccggcgcgga cgaagacgat gaggaagggg
     2881 agacggcgct cgcgagtgcg aactgaaaca cgcgccacgc gggtgtcgta aaatgtgaga
     2941 cgcccgcgcc tgacggcgac gagcgcgcaa gctgccgcca actcgtagta gtgtcctaag
     3001 ttgaatgaat tgctctgcgc cccctcggcg tcctcggcgg ctcggcggtg agtcaactcc
     3061 ggccgtcatt caccgccgag ccgcaaagga cgccgaggga gcgccgagaa ttgaaatcag
     3121 gacactacct actcgtgagc gttcggaaga gtcaatcaca tgaagcgttt cgacggggcc
     3181 accgcaaaga ctttggagtt cgccgcgcgc gtgcgcgccg cgttgcgtcg cacgatgccg
     3241 cgcgtcgcac tggtggcggc gggcctcctg gtgttgtggg tgattctcgg caatacctcg
     3301 attagcgaaa cgcgcgtcga taattttttc gaccgcctga ccttcctcgc cgtcgcctcg
     3361 gtcgcgctct actactgtgt gctcgcgctc cactggacga agcgcaagct cctgtggcgc
     3421 gtgcgccgtc gcctgctcat cacgtacctg ttcgtcgggc tgactccgct cgtgctgctc
     3481 ggcgtgctcg gcatactcgc gtggctcgtc ctgtcggggc aggccatgtc gcgcatcgtc
     3541 aacgtgcaga ttaacgccac ggagcaggtg gcgcaggcga acgccgacgc gctcgccgcg
     3601 gagttcctgc gacggcccat aggcgcgagc gcctctgaga atcaacgctg gctcgacgag
     3661 cggatggcgg tgttgcaggc cggtctgccg ggcgcgcgcg ctgccgtgtg gcgcggcggg
     3721 gccgggcaga gggcgaacac cgaaggcgaa gagttgggcg tcgtcggcga ggggcgcgcg
     3781 gccgacttca tgagcgcggc gcacgggggc gaggaggcgc gcggcatcgg cgtcgagcgc
     3841 gtgtcgttgg acgcgccgct gcccgcgtgg ctcagagggc aggagcgttg gagcggcttc
     3901 gccttcgtcc cgccgccgga cgaatccgcc gacccgttcg gcacgccttc cgtgcgcgca
     3961 ctcgtgcgcc ggggcgaggg cgagcgcggc gtcgcggtgt tgctcgtcgt ccccgtcagc
     4021 cgcgcgctcg tccggcagtt ccgcgagagc acggggattg ccctgcgacc ctacttcctc
     4081 ggcgcgcgca aactcgacgt gaacgtcgcg acgaaaagcg acggcatctc gatcaactcg
     4141 cgccgagaac ctgacgggga gcggcagaag ggcttagagt tgaaccccga cgcgcagctg
     4201 gccgccatcg acaacgcccc cggcgaccgc gaccagttcg gcgaccagct gccgaacggc
     4261 cccccctaca tcgtcgcgct gccggcgact gactggctca acggtgaggt ggacacgcac
     4321 ctctccttcc tcttcgagta ctctctcgcc gaagcgcgcc ggcagctact cgacagcagc
     4381 gccgtcggcg aggtcatcca gaaggcgctc gtggcgctgg ccatcgtctt cctcgcgctg
     4441 gagctggtgg cgctcctcgc cgccgcgtgg atgacgcgcg ccgtgacggg caccgtccac
     4501 aagctccacc tcgccaccca gttcatcaag cgcggcgact tctcacaccg catccgcacg
     4561 cgctcgcacg accaactggg cgagctggcc gccgccttca acgacatgtc ggccaacatc
     4621 gaagtgcttt tggaagagcg cgtcgagcgc gagcgtttgg agcgcgaggt ggagatcgcc
     4681 gccgaggtgc aggcgcaact gttcccgcgc agcctgcccg cgctgacgac cgccgaggtg
     4741 acgggcgagt gccgcgcggc gcgcggcgtg gccggcgact attacgacta cgtggaggtc
     4801 gagcccggtc tggtggcgtt cgtgctcggc gacgtttcgg gcaagggcat ctcggcggcg
     4861 ctcgtcatgt cgaacctaca ggcctcgctg cgggcgcagg tgacgatcat ctcggagcgg
     4921 cgccggctcg tcgggctggc ggcagcgacg accacggccg ccagcgccgc ggcggcgtcg
     4981 gacgggggcg aggggcttgg cgcgtgcggc gtaacggggg cggactctgc ttgcgccgtc
     5041 gagaacatgg tctccagcat caaccagcag ctctgccgca gcaccgactc caaccgcttc
     5101 gccacactct tcctcgcgct ctacgacgac cgcgcccgga cgcttcgcta caccaacgcc
     5161 ggccacaaca cgcccatcct cgtgcgcgcc aacggccggg tcgagcgcct tgacgtgggc
     5221 ggcaccatcg tcggcgtctt cgacttcgtc acctacgagg aggcgcaggc ccggctcgac
     5281 ctcgacgacc tgctcgtcgt cttctccgac ggcatcagcg aggcccagaa cgccgccggt
     5341 gaagagtacg gcgaggagcg catcgtccaa ctcgccgccg ccaaccgcca cctcgcggcc
     5401 gacgacctcc gacataagat tttcgacgag gtcgataagt ggtcaggcga cgaggagcgc
     5461 ggcgacgacc agacgcttgt gattttaaag gccgtgataa gtgatgagtg ataagtgata
     5521 agaaagaaat ctgtttttct tatcacttat cactcatcac ttatcacggc ctttaagccg
     5581 tgaccatcac ggcgcgggcg ccgacgagtt cggcgaagtc tttggtgtag acagttccta
     5641 ccttgcgggc gcggcggatc gtctcgacca tttcgtcggt gttgatgagg ccgtcttcgg
     5701 tgaaggcgac ggcccaggcc gggatgtgcg cggcggcggc gagcagttcc tcgacgtggc
     5761 gcagatactg ctggcgcgtc tcggcgcgcg cgccgagacg accggcgcgc gcgccctcga
     5821 actcgtccca gaaaccatcg ccaccgcgta tccattcctc gcgccaggcc cacgccgtgt
     5881 ggcgcgcgcc gcgccgcgcg ggtcgcggca ggcgcaggaa gaggcagtcg gcctgctcgg
     5941 tggagaggaa gtcgcgcggc tctttgttcg ccgccttgtt cgactctctg ttgtccacgg
     6001 ggcgcggctc cgattcccac aatcgctgga agacgcgcga gagcggctgg cgcagctcgc
     6061 gcgattcgtc gtcgaaggag agcacgtagt cgccgaccat cacgccgagg tggagtgcga
     6121 ggtggcgacg cgtcggcgag gcgagctgtt cggcgttgcg tcggacgttg tcgaaccacc
     6181 atgcgtcggt ctcgccgaac cacttacgca gggccgggtt ggcgaggcgg tagtggggga
     6241 cgtaagcgtc ttcgaggacg agcgccacgt cttcgaccga caggcgctcc gagttgttct
     6301 cgacgcgcgc cgaagccttc gaccacgacc agcgccacac gtcgttcgtc gagacgcgca
     6361 cgccccaacg cttgagcgcg aaatcgaggt cggcctcgcc cgcgaagggg ttggcgagcg
     6421 agcggatttt cagacggcgc agcacgctca gctcgaagcc gagccagccg tcgggcgtct
     6481 gttcaaaatg gtgtgtcgcg ttcatcggtc tcgtccgagg ggcgcctttc tcatcggaag
     6541 tctcacctgc tgtctgactt ttcgagttcg ggcggcgcct gtccgaagct cgcttcaacc
     6601 ggctccggcg gcgcttcgcc ggcgacctct tcacgttgat ccgcctcgtc cgtcacagca
     6661 gactcctcaa cggattgcgc gtccgtcgcg ggcggcgttt gcggctcggg cgctggcggc
     6721 ggcgttgcgc tttgcggctc ggcggcgcgc gcgtaagagc gaagttctgg aatgtctctg
     6781 agcgagagga aggcgcggac gaccgcgggg tcgaactcca gtccggccca gagcgtgagg
     6841 tgcttgcgcg cctcggcctc ggtgccggcg cttcgccatg ggcgggagtc ggtgagcgcg
     6901 gcgtaggagt cggcgacgcg gaggatacgc gccttgagcg gaatctgctc ggcgcgcagg
     6961 cagtcggggt agcccgcgcc gttccaccat tcgtggtgcc agcgaacgag gagttgcacg
     7021 gcgcgctccg cccccgcgcg ccccgactcc tgctcgccga tgacggggtg acgcgcgagg
     7081 tcgaggcgct cctcctcttt gagagagccg gcgcgcgaga tgtagtcgcg cttcatggcc
     7141 atctcgccga ggtcgtgaag cagtgcggcg tggacgagcg acgtgcggtc ggcgcgcgac
     7201 aggccgaagt gccgcgccag ctcgtccgac agacgcgcga cgcgcgacgc gtgcgggtgc
     7261 tcatagccct cgaaggcatc gacggacgcg gccagccgtc tgaacgcctc gccggtcgtc
     7321 cggtcgttct caacaagctc ggttgacatt ggattcagag tttaaagttc aacgcccaaa
     7381 ggtacaagcc gacgggagag ttcaaagttc aaagttcaaa gttcaaagtt cgcggcccga
     7441 ccttgaacct gaaactgcgt ggagaacgga accggctctt ccctttgacc tttgaacttt
     7501 gaactttgaa cttaactttc gcgcagggag cgcagcaggc ggcgggcttc ggcgtgtgtg
     7561 gggttgagcg tgagggcgcg ttcgagttct tcctcggcgc gtcggcgcag tccgatgtcg
     7621 cggtagagtt cggcgagcat cacgcggtag tcggcggcgc gcgggtcgag cgcgacggcg
     7681 gcctggagtt ccgtctcggc gagccggcgc atccggcggt tgtgggcgag cgcgcggccg
     7741 tggtaggcgc ggtagcgcgc ctgctgcggc gcgagcatcg cggcctcgcc gagcagacgc
     7801 gccgccgcct catggtcgtt ctgccgaagc gcctcctgtc cctgtctgaa cttctcctcc
     7861 gcgcccggcg gcacgtcgcc ggcccccgcc gtcttgccgg agacgccgtc gcccccgccg
     7921 gcgctcgcgt ccggcctctg ctcgcgctgt tgctgccgtt gaccgcctga ggcgtcgggt
     7981 gttcgtccca acctgaggtc gtaggccgcg cgcgccttag ggtcgctcaa aacgtcgtag
     8041 gctttggcga tgcgtgcgaa ggccgattcg atgcgcgcgc ggagttcggc gtcggcctcg
     8101 cggctgaagc ggtcggggtg gaagcgacgt gcgagggcgt agtaggcgcg cttgatctcg
     8161 gcgggcggga cggagcggcc cacgccgagc acttcgtagt gcgtgtgccc ggccgcgcgc
     8221 gcaaagagcg tgtcgagtgc ggcgagcggg tcggtttctt cctctgcggc cggctcctcg
     8281 cgaggctgct ccacgctcac aggcggcgct gactctttcg gctcgacagc ctgcgccgcc
     8341 agcgcctgcg cgacttgcgc ggcgtcgagc gccaccggcg cgcgctctcg tcggaggagg
     8401 ccgtagagga agagtgtgta gacggcgcgc agcgtctgct cctcggggag tccggtcatg
     8461 gcggctagtt cggagacgcg caggggcgcg tcaagacgcg agatgacgaa cccctcctcg
     8521 ggctggagcg gcagatcggc cgagagttga ccgggacggc tgacggactc gccgcggtcg
     8581 gggaacctcg cggcgacgaa ctcggcggga agccttcgcg cggcctccaa gagcatctgt
     8641 cccgcgggca gcggcgcgtg tgcgacaccc tgaaggcgtg cgcgcgggtc gaacgcccac
     8701 tcgccctccg tccacagcaa cggcggacgc agggcctcac cgctctgacg cagacgcagc
     8761 gccgccaact cttccgccga gaccgcgccc gaagagacga gcgccgcccc ggccagctcg
     8821 tccgagagcg cgtccacgcc ggcctccaac agacgctcgg gggcgaccgc gccccactgt
     8881 cgcagaagga aagtcaggcg gtgcgcgcgc agattcgtcg cggcgaagac cgcctcgccc
     8941 gcgttgaagt agacgacggc cttgacgcgc tcgcgcgcca aacggaggac gcccgacagc
     9001 ccggcggcgt tgatttcgcg gacgagttcg acgagcgggt gctcgcgcag ttgacccttc
     9061 atgtagcaaa ccacgccggg cgggaaagga gacccggccg cgaaaggata gcacagggaa
     9121 gggagtcagg agtcaggagt cagaataaag agagcgccgc tgcttttatt ctgactcctg
     9181 actcctgact cctgactccc ttcctgattt gacctcgccg ggccaacatc ttatagttcg
     9241 cgacaagaaa acaacacacg attatggctt aatttgcagg cgcggtcgga aaagaccggg
     9301 gccgggtttg ttgtattttt tgagcatgtg gggttgtatg aagatggctt cggttgaggg
     9361 cgcaggcggg gcacggggcg gggagttgga gtcggtcatc gagcgtctcg tggaggagtt
     9421 gcgcgagcgc acggccgaac ttgaaagcgc caacggcgag ttgcaccgca tcggccgcta
     9481 ccgctcgctc tttctcgcgc gcatgtcgca cgagctacgc acgccgctca cctccatcct
     9541 cggcttcgcc gaaatcctcc tcgatcagga agacctcgac gagcgccagc gacgcttctg
     9601 tcagaagatt caggactccg ggatgcgtct ccacgcgagc ctcaaccagc tcgtcgatct
     9661 ctcccgcctc gaagccgggc agacggaact gttcctccac gagttttccc tgcgcgagac
     9721 gctgcgcgag tcgtgcgcgg ccgtggcgcg gctcgccgag aagcaggaag tcaatctcga
     9781 ctgccacacg ccgcccgacc tcagcaacgt cgtctccgac gagggcaagc tccggcaggt
     9841 gctctacaac ttcctcgcgc acgccatcgg ccgcagtccc gccggcgaga gcgtccgcgt
     9901 cgaggtcacg gtcgccgacg ccgggggcct tgccatcgcc atccgcgacg agggcgcgcc
     9961 cctcgaagac ccggagcatc tgtttgaacc cgtggacgtg gacacgccga acgagcgcgg
    10021 cacgaacatg aacgagctgg gcctcgtcat cgcccaccgt ctcatcgacg cgctcggcgg
    10081 cacggtcagc ctgagccacc ccggcggtca cggcctcgaa gtccgcctct ccctgccgga
    10141 gtcgccgcgc gaacgggccg agcttgcgcg ccccgcctga aacatcaaaa gcaacggcga
    10201 gtgacaaccg gcgggtgacg agataaaatc cgcgcaccgg cggcgaagcc tccggcggag
    10261 aaggacccgg aagagcgcga gatgacgagc ctgcccgaga ctaccgagcg aagcgcgagc
    10321 ggcgtcgagg ccgacgcgct gacgatccgc gagtgcgtga gcgtggagga gttggacgcg
    10381 tgcgtccggt tgcagcgcga ggcgttcggg ctgcccgacc ttgagctttc gccgcggcgt
    10441 catctcgtcg tcgcgcgcgc cgcgggcggc tgggtgctcg gcgcttttga cggcgacgcg
    10501 ctcgtcggct tcgtcctcca cctcgcggcg gcgcgcggcg ccgaggtcat cggctactcg
    10561 cacatgatgg cggtcgcgcc cggccagcag aaccgcggcg tcggcgcgcg tctcaagtgg
    10621 gcgcagcgcg cgcgctcgct cgccgagggg cgacgcttca tccgctggac gtgggagccg
    10681 atgcgcgcgc gcaacgcaca cttcaacctc aaccgtctcg gcgtcgtggt gcgcgcctac
    10741 gccgccgact tctatggcgc cgactacgcc gccacgcccg aggagcaggg cgcgcgccgc
    10801 ggcctcgcca gtgaccgcct ctccgccgag tgggaactgc gctcgccccg cgtcgaagca
    10861 ctcgcccgcg gccagcaacc ggcgccgccc ggcgcaccgg ccgctgccgt cgaaattccc
    10921 cccgactgga acgccctcgt ccgccaagac ccggccgccg cgcagcgcgc actgctccgc
    10981 acccgcgacg aattccaacg cgccttcgcc gccggcttag tctgcgccgg cttcgagcgc
    11041 cacgacgcac aacctcgata ccttttttat agtgataagt gatgagtgat aagtgataag
    11101 aaagaaatct gtttttctta tcacttatca ctcatcactt atcacctgtt aaagatgcct
    11161 ttcacacgct acgaccgcta cgttgaccgc cacgcgcgct cgttcgtcga gcggcttcag
    11221 gcgttgtgtc gaatgccttc ggtggcggcg cgcggcacgg ggatgcgcgc cgtggccgag
    11281 acggtcgagc agatgttgca gcgcgccggg gcggggacgc gcacgttcaa gatcggcagc
    11341 ggcttcccca tcatctacgg cgagtgcggg gccgggccgc gctgctttat cgtctacggc
    11401 cattacgacg tgcagcccat cggacaactt tccgagtggt cttcggggcc gttcgcggcg
    11461 tctgtggtcg gcgacaagct ttacgcgcgt ggggcgtcga acagcaaggg cgacttagtc
    11521 gcccgtctcg cggccgtcga ggcttatcaa aagacttacg ggaagctccc cgtctgcctg
    11581 cgcttcctca tcgaaggcga ggacgggctg gccagtccga gtctctaccg cttcgtcgag
    11641 gatcacgccg agatgctgcg ggccgacggc tgcgtctggg acgacggcgc aaaggacacg
    11701 aaggagcgcc tcgtcatcaa cctcggcttc aagggcatct cgttccttga actgcgggcc
    11761 tacggggcgc gcaccgacct ccactcgaag tggggcggca tcgtgcccaa tcccgcgtgg
    11821 cgcatcgtgc aggcgctcgc caccgtcgtc tcgcccaaag gcgtcatcac catcgacggc
    11881 ttctcgcatc acgtcgcgcc gctgaccgcc gaggacgaga agactctgaa gacggtggaa
    11941 ctcgacgagc cgggcctcaa gcgcgagttt cgcatcggcg gctgggtgcg cgggctgacg
    12001 ggcaagcacc tcgcgcgcga actcatcttc ggccccacgg ccaccatctg cggcatccac
    12061 accggccaca cggaggccgg cgccaagacc gtgctgccgg gcacggccgt cgcccgcctc
    12121 gacttccggc tcgtccccga cctcacgcct gacttggtcg tcaaactcct tcgcaagcac
    12181 ctcgacgcgc gcggctttca ggacgtggag atcatcgagc tgggcgcggc gccgacggcg
    12241 aagtcttcgc cccggagctt cgtggcgcgc gccgccgtgg agagcgtggc cgaaatctac
    12301 ggcgcgccgc ccgtcatctt cccactcgat ccggcgagcg gccccgtcgg tgccgtctgt
    12361 ggcgtccagc agccggccac gcccatcgtc tccttcggca tctcctacgc cggctccaac
    12421 ccccacgccc ccgacgagaa catccgcctc gacgacttcc tccgcggcat caagtacttc
    12481 ggccgcatca tccaccgcct cgcgcacgat gaagacaacg acgaggacat gaacgcgcag
    12541 cgcgaagcgc ccgtccagat cagcaagtga cgcttgaagg ggagcgttgt tctggtatca
    12601 ttctattgat tacagcaaga tcgtccccat cgttcacttg agtgtaaggg gaacagattc
    12661 accgcagagg cgcagagaac gcagaggggg cgcggaggct tgaaccggct cgacgcggac
    12721 tgagtaattc aaccctgcgg cgcgaactct gcgtcctctg cgcctctgcg gtgagcctcc
    12781 ttactaccca ttcaactgat cactgctata agtcgcacct gatgattcct taattcatct
    12841 caggccgaat ccgttgcgcc agtcgatagt aaaaactatg atggaaagca aagtttcaat
    12901 aaacacaggt cgggtggtgt aagcgaattg tccgccagcg cgaagagcat tgagagggaa
    12961 gtccaaaggc tgcgtgggga gatactccgc cacgaggagc tgtactacgt caaagacagc
    13021 ccggagattt ccgacgccga gtacgacgcg ctgctcgaga gcctgcgccg gctcgaagag
    13081 gagcacccgg agttgcaggc agcggacagt ccgacgctga gggtgggggg gcgtccggcc
    13141 gagggcttcg aggagtacgt ccaccgccgt ccgatgctgt cgctggagaa cagctacaac
    13201 atcgacgagc tgcgcgcctt cgacgagcgt tgccgcaaac tcgccgacgg ccgcgaattt
    13261 gattatgtcg ccgagctgaa aatcgacggc ctctcgctct cggttcacta cgcggacggc
    13321 gtgctcgcgc gcggcgtgac gcgcggcgat ggccggcgcg gcgaagacgt gacgcagaac
    13381 gtccgcacca tccgcaccat cccgctgcgg ctcaaggacg ggggcggcgc gggcgacgtg
    13441 gaggtgcgcg gcgaggcgta tctgtcgcgc agggagttcg agcgcatcaa cgccgagcgc
    13501 gaggaggcca gcgaggcgcg cttcgccaac ccgcgcaacg ccgccgccgg caccatccgc
    13561 cagctcgacc cgaaggtcgt ctcccgccgc cgcctcgacc tcttcgccta cgactgcatc
    13621 cgcggcgagc gcaaggcgtt cgcgactcac aacgaatcgc tcgaatggct agtgcgcgcc
    13681 ggcttccacg tcaaccctga tcatcgcgtg tgcggaacta ttgaagaggt aatcgccttc
    13741 tgcaacgaaa tggaggccaa gcgcgactcc ttggagtacg agatcgacgg cgttgtcgtg
    13801 aaggtcaact cgacggcgct tcaggaggag ttcggcacga cggcgaaggc gccgcgctgg
    13861 gccatcgcct acaagtaccc ggcccggcag gcgacgacgg agattttaga catcaaggtg
    13921 caagtcgggc gaacgggtgc gctcacgccc gtcgccattc tcgctcccgt tcaactcgcc
    13981 ggcacggtcg tctcccgcgc gacgctgcat aacgaagacg aaatcaagcg gctcgacgtg
    14041 cgtgtcggcg acctcgtgct gatcgaaaag agcggcgaga taattccaaa agttctgaag
    14101 gttctgaaga cgcagggcaa aagaagaaag aaagcttttg agttgccgga taagtgcccg
    14161 gtttgcggcg gcgtcgtctc acggcccgag ggtgaggtcg tctcgcgctg cgtcgcggcc
    14221 gactgcgcgg cgcaactcaa ggcgcggctg cttcatttcg cgtcgcgccg cgcgatgcgt
    14281 atcgaagggc tgggcgacgc gctcgccgaa caactggtgc agaagcggat gatgtgcgac
    14341 gtgggcgacc tctacaagtt gccgttggaa gaggtcgtgg cgctcgaacg catggcggag
    14401 aagtcggcgt cgaacctgat ggcgcaaatc gaggcgagca aggggcgcga cctaccgcac
    14461 ctcgtctacg gcctcggcat ccgccacgtc ggcgagcgca cggccgccat cctcgcgcgg
    14521 cacttcggct cgctcgaacg actctcacag gcgacgctcg aagagttgga cgacatccac
    14581 gagatcgggc tgacggtggc gcagtcggtg cgcgactggt tcgacgacga gggcaaccgc
    14641 gccttgtgcg agcggctgcg acaggcgggc gtccgcaccg aaatggagca gggcattacg
    14701 ggcgtcgcgg cgcgcgacgg cgcgttcgcc ggcaagcagt tcgtcctcac gggccggctg
    14761 gagacgctcg cgcgcgacga ggcgcgcgcg ctcatcgagt cggccggcgg gcgcgtcacg
    14821 tcctccgtca gcaagaagac cgactacgtc gtcgccggcg aggaggccgg ctcgaagctc
    14881 gacaaggcga gagaactcgg cgtcaacgtc atcgacgagg cggcgctcag ggaaatactt
    14941 ggatagctgt tagcggttag ctgttagcga ttagctaaag gccgtccgtt gtcagtagtc
    15001 cgttgtcagt tgtttccgtt ccctggcctg agccttatag tgtgtgcgac tagtcgcccc
    15061 gacaaacaac tgaccactga caactgacca ctgacagtct ttagctgaaa gctaacggct
    15121 aaccgctaaa agctaatagc tattggagct tgaatgaaca aagaagaaca gtcacaaaac
    15181 atcgaactcg tctacacact tttctgcgac gacgtgcggc tggaggtggg caacaagctg
    15241 tcgctgatgg gcgtcttcca tcagatcgcg gtgcagcagt tcccggtctc gttgatgaag
    15301 ttcgccgtcg tcagccagtg gcgcggtgag ggcacgcatc tctccgaggt cagaatcctg
    15361 acggacgacc gccggcagcc cgtcgtggtc gccgagcctt cccgcttcga ggtcatgccg
    15421 ggcggcgtcg ccaacaacat cagcttcttc ttcaacgtcg tcttccacac gcccggccgc
    15481 tactgggtgc agacgctcat cgactccaac ctcaccgacg agcagccgct cacgctcctc
    15541 gccctcgacg cgcaggggat gccccgcctc gacgacgcct ccgaagcggt caactgaaaa
    15601 agccggtgat gagtgataag tgatgagtga taagagaaga acctgtcttc atctcatcac
    15661 tcatcactta tcactcatca ccggcttctt gatgcggatg aatggaatcg aaccatcacg
    15721 gtgtcaccac cacaggcttc tgagacctgc gcgtctacca gttccgccac atccgcgtgg
    15781 gacagcgaaa tcaatataag gacgcgcgcg cgggggtgtc aagcgggacg aaaaggaagg
    15841 caaagtcaaa aaggcggagg gcaaaagtga gggggcgaga ctgcccttcc acttttgccc
    15901 tccgcctttt taagtttggc tttcccgttc tttacggggt gccgaggata tcgccaagga
    15961 tgtcgcggac accagtgcgt cgattggaag agcggccctc gtagccgtcg cggtagccgc
    16021 gcgcgtagcc ctcgcggaag tagccgcggt aggtgtcaac gctgccgtac tcgttgcgat
    16081 a
//
LOCUS       DASVES010000127         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_488415, whole genome shotgun sequence.
ACCESSION   DASVES010000127 DASVES010000000
VERSION     DASVES010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488415"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1252
                     /locus_tag="VF240_04610"
     CDS             242..1252
                     /locus_tag="VF240_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX7174549.1"
                     /translation="MTTETMRAVRIVSHEGAGSLEVGEVERPVAVADRVRVRVRAAGL
                     NRADLLQKRGRYPAPPGAPVDIPGLEFAGEVESVGGEVRAFAVGARVFGITAGGAQAE
                     YVVVPESHLAEAPPGLSWAEAAAVPEVFITAHDAMMTQARLRTGESLLVHAAGSGVGT
                     AAIQLARAAGAGGVYGTARTDAKIERAREFGLEEGVGVGDDAAVFADAVRAWTRGRGV
                     DVILDLVGASYLAANLDALAPRGRLLLVGTLGGADGPLDFRKMMGKRLRVIGTVLRGR
                     SAEEKARAVRRFAAEVVPLLARERLRPVVDSVHAFDDAEAAYARLESNETFGKVVLEM
                     QG"
     gene            1578..2261
                     /locus_tag="VF240_04615"
     CDS             1578..2261
                     /locus_tag="VF240_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX7174550.1"
                     /translation="MEAIPQRRAEIESEFDQRAFEFKAVEARRDEAREKRAALERDLA
                     ETRTRAEKAERDLMSSTNSKAYEAAIREADAAKKQISQLETQTLEQMEVIETAEREMS
                     EREPEVARLRAETDEKLKTFEERSRAQAEELESRRSERERTLGTMPKNISSQYNRIST
                     RIPGGVAVAAARNNSCSACSMKLRPQVMVEVRRGDEIIICENCNRILYYSPPEQQAQT
                     QKAPSVSAS"
     gene            2516..3601
                     /locus_tag="VF240_04620"
     CDS             2516..3601
                     /locus_tag="VF240_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174551.1"
                     /translation="MATTQAAILQNINLNAPPESVTRGGRARWWRRTGTRRGRGFRYE
                     WPDGRPVTDEAALERIRTLAVPPAWREVRVSPSPRGALQAIGLDAAGRVQRVYHASFI
                     ARQQLRKYEKVVRFGELLPALRRRTNEDIESEGLTKQKVLAVVVRLINDLYFRIGSEH
                     SVRRYRTYGVTTLRNRHLDIEDGGRLVFRFTGKHQVRQRRVLVDEELATLMRDLKAVG
                     GARLFEYTDEEGRVRAVRPRDVNEYIKAATSDEFSAKDFRTWGGSLLAAVKLAEARPS
                     EDERESRRNVVRAVRQVAEHLGNTPAVCRSSYVHPAVFDSYRRGVTIEEFRPRRERRR
                     VLGRQPEYQPEELALLKLLRAKPNGQS"
     gene            complement(3898..>5185)
                     /locus_tag="VF240_04625"
     CDS             complement(3898..>5185)
                     /locus_tag="VF240_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX7174552.1"
                     /translation="GFNHKVVSVERLNERADVYCLNVPEYGNFALDAGVFVHNCGMMS
                     ARSGVDVGEATPDKRLKFNRAVMERVAFGAGGKSRRLGMLSGPEFQNLVRGGAEYYVE
                     KYGATFDRSRAERHRIPVDDGWQIPWGGKGKPERGQEQLGSLGSGNHFIELQRAEETG
                     TLFVQVHTGSRGFGHGLATNYFELAREERPTEIRDIDLGYFTPDSKHYRDYLNAVAAG
                     GNYAILNRLVIFEQVSEAFREVFGEELELVYEISHNLVQREWHPEFGEVWVHRKGATR
                     AFPAGHPGLKGTHWEDKGHPVLIPGSNKDWSYILRPLAGAAASGYSVNHGAGRRMSRG
                     EASRTLSQRAVDDEYAEAGIVVNTDGRVPLDEAAPAYKSSQEVIQAVVGAGLAEIVHR
                     LWPLASLKGVDERKGGRRKGKGRPDKGAGKKTSQHY"
BASE COUNT          918 a         1677 c         1701 g          889 t
ORIGIN      
        1 tggttgacga cgacgagcag gcttgcccct tcgctcatgc gcggccactc taccacagcg
       61 gacgccgcgt gccgcgcggt ggtatcttca aggaattgaa gctgcgaaaa gtcaattaac
      121 atagatggac aggacggaca agatgaagaa aggatgattt gaaatttcag atttcagatt
      181 tcaattcttc tgatcctgct catcctgtcc atccatgtaa ttttttgatg gagtgtgagc
      241 gatgacgacg gagacgatgc gggccgtgcg gattgtgtcg cacgaggggg cggggtcgct
      301 ggaggttggc gaggtggaga ggccggtagc ggttgctgac cgtgtacgcg tgcgggtgcg
      361 cgcggcgggc ctcaatcgcg ctgacctgtt acagaagcgc gggcggtatc cggcgccgcc
      421 gggcgcgcca gttgacattc cgggccttga gttcgccggc gaggtcgaga gcgtgggcgg
      481 agaggtgcgc gcgttcgcgg tgggcgcgcg cgtgttcggc atcacggccg gcggggcgca
      541 ggccgagtat gtggtcgtgc ccgagagcca tctggcggag gcgccgcccg gtttgagttg
      601 ggccgaggcg gcggccgtcc ccgaggtctt catcacggcg cacgacgcga tgatgacgca
      661 ggcgcgcctg cgtaccggcg agtcgttgct cgtccacgcc gccggctcgg gcgtcggcac
      721 ggccgcgatt caactcgcgc gcgcggccgg cgcgggcggc gtctacggga cggcgcgcac
      781 cgacgcgaag atcgagcggg cgcgcgagtt cggccttgaa gagggcgtgg gtgttggcga
      841 cgacgcggca gtgttcgccg acgcggtgcg cgcctggact cgcgggcgcg gcgtggacgt
      901 gattctcgac ctggtcggcg cttcctacct cgccgccaac ctcgacgcgc tcgccccgcg
      961 cggacgcctg ctgctcgtcg gcacgctcgg cggggccgac ggcccactcg actttcggaa
     1021 gatgatgggg aagcgtctgc gcgtcatcgg cactgtcttg cgcggacgct ccgcggagga
     1081 gaaggcgcgc gccgtgcggc gcttcgccgc ggaggtcgtg ccgcttctgg cgcgcgaacg
     1141 tttgcgcccc gtcgtcgata gtgtccacgc gttcgacgac gcggaggcgg cctacgcgcg
     1201 tctcgaatcg aacgagactt tcgggaaggt cgtgctggag atgcagggat gagggcgggg
     1261 ggatgagtaa aaagccgtca atcgtgaatc gtcaatcgtg aatcgaagaa acgtctttta
     1321 cgattgccga ttcacgattg acgatttacg gctcttattc atccctcatc cctgccttcc
     1381 tacgccgccg cttgcatgcg ggggaggtgt aaagggataa tgctgctgaa ctaattctga
     1441 cgggcgcgcc ggagacggcg gagcgagagc gcccacggcc gatattttca aggagatgaa
     1501 atatagtgag agcggaattg cagcagttag tcgctttaca aaacgcggag accgcaatac
     1561 gtaaattaca agccgagttg gaggcaatcc cacaaaggcg cgccgagatc gaaagcgagt
     1621 tcgatcagcg cgcctttgaa tttaaggccg tcgaggcccg ccgcgacgag gcccgagaaa
     1681 agcgcgcggc gcttgagcgc gatctggccg agacccgcac ccgcgccgag aaggctgagc
     1741 gcgatttgat gtcctccacc aactccaaag cctacgaagc cgccatccgc gaagccgacg
     1801 ccgccaagaa gcaaatctct caactcgaaa cacagaccct cgaacagatg gaggtcatcg
     1861 aaacggccga gcgcgagatg agtgagcgcg agccggaggt ggcccgcctg cgcgccgaga
     1921 cggacgagaa attgaagacg ttcgaggagc gctcacgcgc gcaggccgaa gagctggagt
     1981 cgcgtcgctc ggaacgcgag cgcacattgg ggacgatgcc caagaacatc agcagccagt
     2041 acaaccgcat cagcacgcgc atccccggcg gcgtggccgt ggccgccgcg cgcaacaact
     2101 cctgctcggc ctgctccatg aaactccgcc cgcaggtgat ggtcgaggtg cgccgcggcg
     2161 acgagatcat catctgcgaa aactgtaacc gcatcctcta ctactccccg cccgagcagc
     2221 aggcgcagac gcagaaggcg ccgtccgtgt cggcatcgta ggcgaacgcc cccactaaag
     2281 aaggcaaaag taaaaaggca aaaggcaaaa gtgataaggc gtgtgcctct tcacttttgc
     2341 cttttgcctt tttacttttg ccttcctttt cccagtggcc cggttcttgt ctccatgcac
     2401 gtcgcacatc ggggtcgcag ggcacgcccc tcggccccgg cggggttgat tactcccacg
     2461 ctgtcacaac ttaaagactg agggttgagg cgggcgaaca gggggagaga gacggatggc
     2521 gacgacgcag gctgcaattc tacaaaacat caacttgaac gcgccgcccg aaagcgttac
     2581 acgcgggggc cgcgccagat ggtggcggcg gacgggcacg cgccggggcc ggggcttccg
     2641 atacgagtgg cccgacggcc gccccgtcac cgacgaggcc gcgcttgagc gcattcgcac
     2701 gctcgccgtc ccgcccgcgt ggcgcgaggt gcgcgtctcg cccagcccgc gcggcgcgct
     2761 gcaagccatc ggcctcgacg ccgccggtcg cgtgcagcgc gtctaccacg cgtcgttcat
     2821 tgcgcggcag cagttgcgaa agtatgaaaa ggtcgtgcgc ttcggcgagc tgttgccggc
     2881 gctgcgtcgc cgcaccaacg aggacatcga gagcgagggg ctgacgaagc aaaaggttct
     2941 ggccgtcgtc gtgcgcctca tcaacgatct ctacttccgc atcggctccg agcacagcgt
     3001 gcgacgctac cgcacctacg gcgtgacgac gctgcgtaac cgccacctcg acatcgagga
     3061 cggcggccgg ctcgtcttcc gcttcacggg caagcatcag gtgcgccagc gccgcgtgct
     3121 cgtcgatgaa gagttggcca cgctcatgcg cgacctcaag gccgtgggcg gcgcgcgtct
     3181 cttcgagtac acggacgaag aggggcgcgt gcgcgccgtg cgtccccgcg acgtgaacga
     3241 gtacatcaag gcggccactt ccgatgagtt ctccgccaag gactttcgca cgtggggcgg
     3301 gtcgttgctg gcggccgtga agctggcgga ggcgcggccc tcggaagacg agcgcgagtc
     3361 gcgccgcaac gtcgtgcggg cggtcagaca ggtggccgaa catctcggca acacgccggc
     3421 cgtctgccgc tcctcttacg tccacccggc cgtcttcgac agctaccggc gcggcgtcac
     3481 catcgaggag ttccgcccgc gccgcgagcg ccgccgcgtc ctcggccgcc agcccgagta
     3541 ccagcccgaa gaactcgccc tgctcaagct gctgcgcgcc aagcccaacg gtcaaagtta
     3601 aaggcaaaag tgaggaagcc gaactgcgag cgtcaaggag ccgcggagcg gcggcagaat
     3661 gtagccccgg ccgtgagacc ggggttaacg cgggacataa ttaacgaagc cccggagggg
     3721 cggaagaacc tcaacgagcc tgatgctcaa gattgaaagt tctttcgccc ctccggggct
     3781 tcgatttctt tccttgcctg tccccacggc tcacgccgtg ggctacattc tgccgccgcc
     3841 tccggcggct cgttgacgcc tgcgacttgg ctttataact tttacctttt gccttcttca
     3901 gtaatgctgg ctcgtctttt tgcccgcgcc cttgtccggc cggcccttgc ccttgcgccg
     3961 gccgcccttg cgctcgtcca cgcccttcaa agaggcgagc ggccataggc ggtggacgat
     4021 ctccgccagc cccgcgccga cgaccgcctg aatgacctcc tgcgaagact tgtaggctgg
     4081 cgcggcctcg tccagaggca cgcgcccgtc cgtgttgacg acgatgccgg cctccgcgta
     4141 ctcgtcgtca acggcgcgct gcgagagcgt gcgcgacgcc tccccgcgcg acatccgacg
     4201 ccccgcgccg tggttgacag agtagccgct cgcggcggcc ccagcgagcg gccgcaggat
     4261 gtagctccag tccttgttgc tgccggggat gaggacgggg tggcccttat cctcccagtg
     4321 cgtccccttg aggccggggt gtccggccgg gaaggcgcgc gtcgcgccct tgcgatgcac
     4381 ccacacctcg ccgaactccg ggtgccactc gcgctgcacg aggttgtggc tgatctcgta
     4441 gaccagttcc aactcctcgc cgaacacctc gcggaaagcc tccgacacct gctcgaagat
     4501 gacgaggcgg ttgaggatgg cgtagttgcc cccggcggcg acggcgttca aataatcacg
     4561 gtagtgtttg gagtcgggcg tgaagtagcc gaggtcgatg tccctgattt ccgtgggccg
     4621 ctcctcgcgc gccagctcga aatagttcgt cgcgaggccg tggccgaagc cgcggcttcc
     4681 ggtgtgtacc tgcacgaaga gcgtccccgt ctcctcggcg cgctgcaact cgatgaagtg
     4741 gttgcccgac ccgagactcc ccaactgctc ctggccgcgc tcgggtttac ctttgccgcc
     4801 ccacggaatt tgccagccgt catccacggg gatgcggtgc cgctcggcgc ggctccggtc
     4861 aaacgtcgcg ccgtattttt cgacgtagta ctcggccccg ccgcgcacca gattttgaaa
     4921 ctccggcccc gacaacatgc cgagccggcg actcttaccg cccgccccaa aagccacacg
     4981 ctccatcacg gcccgattga atttcagtct cttgtccggc gtggcctccc cgacatcaac
     5041 gcctgagcgc gcgctcatca tcccgcagtt gtggacgaac acgcccgcgt cgagcgcgaa
     5101 gttgccgtat tcggggacgt tcagacagta aacgtccgcc ctttcgttta gcctttcgac
     5161 ggaaacgact ttgtgattga acccg
//
LOCUS       DASVES010000128         7624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1583386, whole genome shotgun sequence.
ACCESSION   DASVES010000128 DASVES010000000
VERSION     DASVES010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583386"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..3678
                     /locus_tag="VF240_04630"
     CDS             496..3678
                     /locus_tag="VF240_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HEX7174553.1"
                     /translation="MKLIRQTVLIYREGSSDKVYEVDLCEAGAGSYVVNFRYGRRGAR
                     FKEGVKTVAPVPLLDAEKIFLDLVNSKSKKGYREINAQAAEPPVRNVTSGPSDRDAQA
                     VAIIARLRDRAEATKRGWPLERVIWRAGELKLREAAPQLVALVGSGSVLRDYCIAWAL
                     GWCGGPESVSTLGRLYDDPSAADFVRRIAGEALLKLSDEGTRAEFTSRALAALPGQLR
                     GPAVGGPSGVFAAALRDYLDAGDHRRYATLDALYLIDNEHTRPALLELLRTAPLRPNY
                     FQRIRHIFKAAEYRHDAEVYGLIAYRFEKEKAMFGKTYSPLAGEPRWSYVTLPGGSYF
                     SDAHKVIKKEDAPIAYGGRTRAYLRQRVWRTLRRLGELGDAEGYVRVAAGVILPFTDA
                     DADEPREIGVFQWRTGRTTTACWDAYASYWAFNHILYANSPRYFRREGTSAWRCRAPY
                     KPGDPEPDAREEAFPELWEREPRGLLHLIAESNCRPVQLFAVKALRACVQFTAELDRE
                     ALLMILGRPSEVIAHFGFELARARYRPAEPDHALVLAVANSASPEARAEAHLWIDEGR
                     ERFLRDGDFTFALVTSPRPDTREFASRLLRSTALPAEEARALVVRLIAHLVGLDATRG
                     EEARDVAATLLECFGQQLRTLGLGPVLDMLKHPLAAVQEFGGQILLGHEVRPAELPEE
                     IVLSLINSPHESLRGIGVRLLGELPDESLLEREGLLVALARHPLADLRNAVRPVIRRL
                     SRSAAGSPFTRRLAQLLLTTLLAPETSEGVHRSVALLLQDDLGDAWAGKVEKDLALRL
                     ALASSPAARELGGYLVDTRARADAAWAQSFTTDEIVEMAAAEVFAVRQAARTLFDLLI
                     DRYRQATNPDGHLDEMARAVRLLDTGWEDARAFYFEAFRTRLGAQDFTPGILVSVCDS
                     VRAEVQQFGRELVTKYFSEEAGQEYMLKLSEHPSAELQLFVTNYLERYCAGDSARLRE
                     MSHYFTSVLSRVNKARVAKARVIVFLTSEAQKSEAAARAVAEILARQSATVAVGTRAA
                     AIEAMLAIRRAYPNVPLPLEIKPAEVRRAV"
     gene            3668..5323
                     /locus_tag="VF240_04635"
     CDS             3668..5323
                     /locus_tag="VF240_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174554.1"
                     /translation="MLFEYTYRGSSAVASTADRTRMSFAPDVTREPTFFTGRLRQRIE
                     FREAISALHDVVVSDLRFKPPDKTAYKEWAARQEQIDWQAIAAERTQVIGRVRELQAE
                     LSDLHARSSYRMAPFNKARRAYYEYILKTEKDFWRVLFDPVITVHPDEVFFECFSQDE
                     SSYGRLGAGYEVFEEIGEFACGTTNIDYSAALYDEFQKIRTYKETRFEIDPSGFDVRT
                     TGEAEYREVKIDLPDSWVRGFLQVSSAMSLPARTFDLHPMDIHNICFVLRRRREQKGP
                     RAMRYHLTPGEPVKVVFEPWETEIVCARSVYAGADAEVIRVWGRRRLHILERLIPVAR
                     KFTVHLLGTGLPSFYVADLGDMSFTLGLSGWTANDWSRAGNFDLLAPRAEVDELTKRV
                     VFDELRREWSATPDELASRLHLDRPTVLGALGAYTQAGRAIFDLNKRIYRARELSREP
                     LPVERLRFSSEREESAARFLAERAVELAATQSNTEGQLSLRGVVKDKEKEKTFHPSLA
                     LDADGRMVRAECTCNWHQQHKLFRGPCEHILALRMEAARRRQI"
     gene            5628..7160
                     /locus_tag="VF240_04640"
     CDS             5628..7160
                     /locus_tag="VF240_04640"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEX7174555.1"
                     /translation="MSTERPKTREELYERIRQGGREEFILEDMIRLGFWPARGEMPQD
                     PAEEIHRRSELQRELAELRAEHRRLYNEEALIKELRLRRFAESRRKRQETKERRERER
                     RERAEAWRQRQEREIVYLGEGVSGGLNRTECDAPRLESYGLQPLGSAAEVAAALGVTV
                     NELRFLAFDRKTSTVSHYVSFQIPKKTGGLRHISAPMPRLKAAQHWVLHNILEKVEAH
                     DCAHGFRGGRSIVTNARPHVGAEAIINLDLQDFFPSISYRRVKGVFRAFGYSEAVATI
                     LGLLCTQPTTEEVEFDGRTYFVATGARHLPQGAPTSPAITNILCRRLDRRLLKAAEDL
                     GFTYTRYADDMTFSASGESLRHLGGVLDRVKAIVQHEGFTVHPDKTRVLRRSRQQEVT
                     GVVVNSKLNVSRRELKRFRATLYQIEKDGPVGKQWGRSPDVIASLQGYANFVYMVNPA
                     KGAELQQRVRAVAEKYGWEPAKVVRPSPSKRSGPPRPGTASPQSETSQPTPARKKWWK
                     LW"
     gene            7305..7565
                     /locus_tag="VF240_04645"
     CDS             7305..7565
                     /locus_tag="VF240_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174556.1"
                     /translation="MKRIGSALLGLSVFVMIALANGSAAPDSKYRVFCANNKIEVEQR
                     TLEQQKNARGSNVCMLAEFDYASDADKYAEGQGGKGADCKCP"
BASE COUNT         1388 a         2505 c         2423 g         1308 t
ORIGIN      
        1 gcgattggca gaactgctga acgagtggaa ggcgcgccgg tgatcaaaga cgcgccacct
       61 aacaatgaat tcatgcttct tcatccttca ggatattcct atagaaaaag ctgttcctaa
      121 gttgtatatt aattgaggga ccttttgtcg ccattgtgaa gtagaggttc gctcgttttc
      181 attccggcgt ctttgtccca gctatcgccc ctaccacttc acctgacgtt tacgaagctt
      241 cggatgcagg ctcacttagc agtgcctgga ttaacccccg caaattgttg gaagggtttc
      301 cggattagca atgttgtatc acacatgaaa caaagtattg cactcaacac aaccttgtgt
      361 tatatcattg acggttgccg cggttctcgt attttgagta agtgttgcag cagttgccgc
      421 cagcataagg gtaaacatag taaagcgtta aaggtctcgg gttaatactt cacccgtttc
      481 aggtcgtggc tcgccgtgaa gctgatcagg caaacagttc taatctaccg ggagggaagt
      541 tccgacaagg tctacgaagt cgatctctgc gaggccggcg ccggcagcta cgtcgtcaat
      601 ttccgttacg gccgccgggg ggctcgcttc aaagagggcg taaagactgt tgcgcccgta
      661 ccgctgcttg acgccgagaa aatattcctg gacctggtca attccaagtc gaagaagggt
      721 taccgcgaaa tcaacgccca agctgcggag ccgccggtga gaaacgtcac ttccggcccg
      781 tcggaccgcg acgcacaggc cgtcgctatc atcgcccggc tgagagatcg cgcggaggct
      841 acgaagcgcg gctggccgct cgaacgcgtc atctggcgcg cgggcgagtt gaagctgcgc
      901 gaggcggccc cgcagctcgt cgcgctggtg ggcagcggta gcgtgctgcg cgactactgt
      961 attgcctggg cgctgggctg gtgtggaggc cccgaatccg tttccactct cgggcggctt
     1021 tatgacgacc cttccgccgc cgacttcgtt cgacgcatcg ctggcgaagc cctcctgaaa
     1081 ctctcggacg agggtacgcg ggcggagttc acaagtcgcg ctctagctgc cctgcccgga
     1141 caactccggg ggccggcggt gggcgggccg tccggcgtct tcgccgcagc gctccgggac
     1201 tacctcgacg cgggcgacca ccgccgctac gcaacactcg acgcgctcta cctgatcgac
     1261 aacgagcaca cgcgcccggc cctgctcgaa ctgctgcgca ccgcgcccct caggccgaac
     1321 tacttccagc gcatccgaca catcttcaag gccgcggagt accgacacga cgcagaggtc
     1381 tacggcctca tcgcctaccg cttcgagaaa gagaaggcga tgttcggcaa gacttactcc
     1441 ccgctggcgg gagagccgcg gtggtcttac gtcacgctgc cgggcgggtc ttacttctcc
     1501 gacgcacaca aggtgatcaa gaaagaggac gcgcccatcg cctacggcgg tcgcacgcgc
     1561 gcctacctgc gccagcgggt gtggcgcacg ctccggcgtc tgggcgagtt gggcgacgcc
     1621 gaggggtacg tgcgcgtggc ggcgggcgtc atcctcccct tcaccgatgc ggacgcggac
     1681 gagccgcgcg agatcggcgt tttccagtgg cggactggcc gcaccacgac ggcgtgctgg
     1741 gacgcctatg cttcctactg ggccttcaac cacatcctct acgccaacag cccgcgctac
     1801 ttccgccgcg agggcacttc cgcatggcgc tgccgcgcgc cttacaagcc cggcgacccg
     1861 gagccggacg cgcgcgaaga ggcgttcccc gaactgtggg agcgcgagcc gcgcgggctg
     1921 ctacacctca tcgccgagag caactgccgc cccgtccagc ttttcgcggt caaggcgctc
     1981 cgcgcatgtg tgcagttcac cgccgaactc gaccgcgagg cgctgctgat gatcctcggc
     2041 cgcccctccg aggtcatcgc acacttcggc ttcgagctag cgagggcgcg ctaccgcccg
     2101 gcggagccgg accacgcgct cgttctcgcg gtcgccaaca gcgcctcgcc ggaggcgcgc
     2161 gccgaggcgc acctctggat cgacgagggg cgcgaacgct tcctgcgcga cggcgatttc
     2221 acattcgcgc tcgtcaccag cccacggccg gacacgcgcg agttcgcgag ccgtctgctg
     2281 cgctcgaccg cgctccccgc cgaggaggcg cgcgcgctcg tcgtccgcct catcgcccac
     2341 ctcgtggggc tggacgccac tcgcggcgaa gaggcccgcg acgttgccgc gacgctgctc
     2401 gaatgtttcg ggcagcagct ccgcacgctc ggcctcggcc ccgtcctcga catgctcaag
     2461 cacccgctcg ctgcggtgca ggagtttggc ggccaaattc ttctggggca cgaggtgagg
     2521 cccgccgagt tgcccgaaga gatcgtcctc tccctcatca actccccgca cgagtctctg
     2581 cgcggcatcg gcgtcaggct gctcggcgag cttcccgacg agtctctgct cgaacgcgag
     2641 ggcctgctcg ttgcgctcgc ccgccacccg ctagccgatc tgcgcaacgc cgtgcgcccg
     2701 gtcatccgcc gcctgagccg tagcgcggcg ggctcgccgt tcacgcgcag gctcgcccaa
     2761 ctgctcctga ccactctcct cgcgcccgaa acttccgagg gcgtccaccg aagcgtcgcg
     2821 ctactgctgc aagatgactt gggcgacgcg tgggctggca aggtcgagaa agatttagca
     2881 ttgcggctgg cgctcgcctc ttcccccgcc gcgcgtgagt tgggaggcta tctcgtcgat
     2941 acgagggcgc gcgcggacgc cgcctgggcg cagtcgttta cgacggacga gatcgtcgag
     3001 atggccgccg cggaagtgtt cgccgtgcgc caggccgcgc gcaccctctt cgacctgctg
     3061 attgaccgct accggcaggc caccaatccc gacggacatc ttgacgagat ggcgcgcgcg
     3121 gttcgcctgc tcgacaccgg atgggaagac gcgcgcgcct tctacttcga ggcgttccgc
     3181 acgcgcctgg gcgcacagga cttcacgccg ggcattctgg tgagcgtctg cgacagcgtg
     3241 cgcgcggagg tgcagcagtt cgggcgggaa ctggtgacga aatatttcag cgaggaggcg
     3301 ggccaggagt acatgctcaa gttgagcgaa cacccgtcgg ccgagttgca actgttcgtc
     3361 accaactacc tcgaacgcta ctgcgccggc gactcggccc gtctgcgcga gatgtcgcac
     3421 tacttcacga gcgtgctctc gcgcgtgaac aaggcgcgcg tggcgaaggc ccgcgtgatt
     3481 gtgttcctca cttccgaggc gcagaagagc gaggcggcgg cgcgcgccgt cgccgagatt
     3541 ctcgcgcgtc agtctgcgac cgtggcggtc gggacgaggg cagcggcgat tgaggccatg
     3601 ctggccatac gccgcgcgta tccgaacgtc ccgctaccgc tcgaaatcaa gcccgcggag
     3661 gtgcgccgtg ctgtttgagt atacctaccg cggcagcagt gccgtcgcca gcacggccga
     3721 ccggacgcgc atgtcgttcg cgcccgacgt gacgcgcgag ccgacgttct ttacgggccg
     3781 cctgcgccag cgcattgaat tccgcgaggc gatcagcgcg ctgcacgacg tggtcgtctc
     3841 cgacctccgg ttcaagccgc cggacaagac cgcctacaag gagtgggccg cgcggcagga
     3901 gcagatcgat tggcaggcca tcgctgccga gcgcacgcag gtcatcgggc gggtcagaga
     3961 gttgcaggcg gagctgagcg accttcacgc gcgcagctca taccgcatgg cgccattcaa
     4021 taaggcgcgg agggcgtatt acgaatacat ccttaaaacg gagaaggatt tctggcgcgt
     4081 gctctttgac cccgtcatca ccgtccaccc cgacgaggtg ttcttcgagt gcttcagcca
     4141 ggacgagtcg agttacgggc ggctgggcgc gggctatgag gtcttcgagg agatcggcga
     4201 gttcgcctgc gggacgacga acatcgacta ctcggccgcg ctctacgacg agttccagaa
     4261 gatacgcacc tacaaagaga cacgcttcga gatcgacccc tcgggcttcg acgtgcggac
     4321 gacgggcgag gcggagtaca gggaggtgaa gatcgatctg cccgacagtt gggtgcgcgg
     4381 cttcctccag gtcagctcgg cgatgtctct gccggcgcgc acgttcgacc tgcacccgat
     4441 ggacatccac aacatctgct tcgtgctgcg ccgccgtcgc gagcagaagg ggccgcgcgc
     4501 gatgcgttac cacttgacgc cgggcgaacc cgtcaaagtg gtcttcgagc cgtgggagac
     4561 tgagatcgtc tgcgcgcgct cggtctacgc gggcgcggac gccgaagtga tccgcgtctg
     4621 gggccgccgg cgcctccaca tccttgagcg tctgatcccg gtcgctcgca agttcacagt
     4681 tcatctgctt ggcacgggcc taccctcgtt ctacgtggcc gatttgggcg acatgtcgtt
     4741 cacgctgggc ctttcgggct ggacggcgaa cgactggtca cgcgcgggca acttcgatct
     4801 gctggctccg cgcgccgagg tcgatgaact gaccaaacgg gtcgtcttcg acgagttgcg
     4861 ccgggaatgg agcgcgacgc ccgacgagtt ggcctcgcgg ctccacctcg accgcccgac
     4921 cgtgctcggc gcgctcggcg cgtacacgca ggcggggcgc gcgatcttcg acctgaacaa
     4981 acgcatctac cgcgcgcgcg agttgagccg cgaaccgttg cccgtggagc ggctgcgctt
     5041 ctccagcgag cgcgaggaga gcgcggcgcg cttcctcgcc gagcgggccg tcgagcttgc
     5101 cgcgacgcag tcgaacacgg aaggacagct ttccttgcgg ggcgtcgtga aggataagga
     5161 gaaggagaag actttccatc cgtcgctcgc gttggacgcg gacggacgta tggtgcgcgc
     5221 cgagtgcacc tgcaactggc accagcagca caagctcttc cgggggccgt gcgagcacat
     5281 cctggcgctg cggatggagg ccgcgcgccg gcggcagatt tgacaatcag gttcgattgc
     5341 caggcgtaac ggccagggcg cttcccgtgg gccttgcaac ccggacggtg gatcgccgtc
     5401 catgcgtaaa gctcagggca tgcatcactg gtccgctctt aactgtgcgg gtcaggccac
     5461 ctgtgcgttt cccggtgtgg ctttcacgaa gcggaagacg cacaggtggc ccgccccgct
     5521 cgagttgagc gacccagtcc tcaggagttt tcgaagggga cgcgggcaag ccctggccgt
     5581 tacgcgtggc aatcgagccc ccaacacccg aacgcacgga ctatccgatg tcaaccgaac
     5641 ggccgaaaac ccgcgaagag ctgtacgagc gcatccgtca gggggggcgc gaggagttca
     5701 tcctcgaaga catgatccgg ctcggcttct ggcccgcccg cggcgagatg ccgcaggacc
     5761 cggccgagga gattcaccgg cgctccgagt tgcagcggga gctcgccgag ctgcgtgccg
     5821 agcaccgccg cctctacaac gaagaggcgc tcatcaagga gctgcgcttg cggcggttcg
     5881 ccgagtcgcg ccgcaagcgg caggagacaa aggagaggcg cgagcgcgag cggcgggagc
     5941 gcgccgaggc gtggcggcag aggcaggagc gggagatcgt ctacctcggc gaaggggtct
     6001 cgggcgggct caaccgcacc gagtgcgacg cgccacgcct cgagagctac ggcctccagc
     6061 cgctcggctc cgcggccgag gtcgcggcgg cgctcggcgt caccgtcaac gagctccgct
     6121 tcctcgcctt cgaccgcaaa acctcgaccg tctcgcacta cgtcagcttc caaattccga
     6181 agaagaccgg cggtctgcgc catatctccg cgcccatgcc gcgcctgaag gcggcgcagc
     6241 actgggtgtt gcacaacatc cttgagaagg tcgaggcgca cgactgcgcc cacggcttcc
     6301 gcgggggaag atccatcgtg acgaacgcgc gcccgcacgt cggggcggag gcgatcatca
     6361 acctcgatct gcaagacttc ttcccctcga tcagctaccg gcgcgtcaag ggggtcttcc
     6421 gcgctttcgg ctactcggag gcagtggcca ccatcttggg gctgctctgc acgcagccca
     6481 cgaccgagga ggtggaattc gacgggcgca catatttcgt cgccaccggc gcacgccacc
     6541 tcccgcaggg cgcgccgacc agcccggcga tcaccaacat tctctgccgc cgcctcgacc
     6601 gccgactgct caaggccgcc gaggatttgg gtttcactta tacgcgctac gccgacgaca
     6661 tgacgttctc ggcttccggc gagagcctgc gccacctggg gggcgtcctc gaccgcgtga
     6721 aggcaatcgt ccagcacgaa gggtttaccg tccacccgga taagacgagg gttctgcgac
     6781 gctcccgaca gcaggaggtg accggcgtcg tcgtcaacag caagctgaac gtctcgaggc
     6841 gagaactcaa gcggttccgt gccaccctct accagatcga aaaggacggt cccgtcggga
     6901 aacaatgggg acgctcgccg gatgtaatcg cctcgctcca gggatacgcc aacttcgttt
     6961 acatggtgaa ccccgccaag ggtgcggaac ttcaacagcg tgtccgtgcc gtggcagaga
     7021 aatatggctg ggagcctgcc aaggtcgtgc ggccttcacc ctctaagaga tcagggccac
     7081 cgcggcccgg cacggcctca ccgcaatcag agacttccca acctaccccc gcccggaaaa
     7141 agtggtggaa actttggtga agaccgtgac ggctaacggg tgagtttaca taaccactct
     7201 tgactgtttc caatacatca aaaacattga caccaccatc gcgttcggtg tagaagcaat
     7261 ccaccttcgc tacacagcga cttccataaa gaatgaggtc taacatgaag agaataggca
     7321 gtgccctatt agggctgagt gttttcgtga tgatcgcgtt ggctaacggc tcggccgcac
     7381 ctgactcgaa ataccgtgtc ttttgcgcca acaacaagat tgaagtcgag cagcgcaccc
     7441 ttgagcagca aaagaacgcc cgtggatcaa acgtctgtat gctggctgag ttcgactatg
     7501 cctcagacgc cgacaaatac gccgagggac aaggcggcaa aggcgctgac tgcaagtgcc
     7561 cgtaaggctt gtgaataacc ccatcaaaag ctcatcagac ccaaccatga ccgtcctctt
     7621 taga
//
LOCUS       DASVES010000129        14074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1669422, whole genome shotgun sequence.
ACCESSION   DASVES010000129 DASVES010000000
VERSION     DASVES010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669422"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VF240_04650"
     CDS             <1..405
                     /locus_tag="VF240_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX7174557.1"
                     /translation="ELGKSDDVRLTARLMPQVGWVMRHAAGTGAATPLGLKLVDYFRE
                     RGYEVTYVGGEQGAMATEKYAVERAMYELRWQGANVVALAPGRVVAFEHNVHTNEALR
                     RAGVDVVTFPGELLSIRNGGPHCLVMPLVRGD"
     gene            complement(724..2118)
                     /locus_tag="VF240_04655"
     CDS             complement(724..2118)
                     /locus_tag="VF240_04655"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX7174558.1"
                     /translation="MKKTKIVRVTSVGVEKTYNMTMRGPHHNYFVNGILTANSHSVAY
                     AHVAYQTAYLKANYPEHFYAAVMTYCTDDAAKIFKYASELRAQGIKMLPPDVNESGAG
                     FTPVEGAIRYGLSAIKGLGQSAVGAISAGRAERPYTSIFDFEERVGEKGSGKRVLESL
                     VCAGAFDSMKPAAQTLHHWRACLFASVDRALEGGSRTRRERDSGQVAMFGFVAGGAAE
                     ESNAPTHMFAAAEAWAPKDLLTREKAAVGFYVSGHPMQDFAERIEQLGCASVADLSGA
                     APDARLRLAGVVSDFTVRNTKKGDRYAFFRLEDMTGVSVKCVLWPEALKMKGGDAADD
                     RVVLAVGRIDGSGEGSVTLVCDEVMLLEKARVPAHVPAAGFRNGRNAGKALVIEMPAA
                     SADLATLCDSVTQALVSNPGDCEVFIEMNLPDGLLVRARTNRLIKVFPSQKLQDALAS
                     TGARIRWADASSMN"
     gene            complement(2121..2900)
                     /locus_tag="VF240_04660"
     CDS             complement(2121..2900)
                     /locus_tag="VF240_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX7174559.1"
                     /translation="MTPEVEHFIVEKYKGGLSAGEILKSIPFKTRKTVYDVLEKHGVP
                     KRCGIADYKTCDEAAFAVIDSPAKAYWLGLLISDGYVIDGGPGRTPQVGLQMTDREAV
                     EGFRNFLGLKNPVLRIAPRGERHRPMYRVICHSRRMASDLARHGVIPRKSLCTYLPIL
                     AEDLMPHLIRGIFDGDGTVSRRTDGEVIVGFCGSERLVAEVRMWLICRLGVSDNRVHE
                     NGAIRYVQWSHKSDVRQIARCLYREAQQYLERKLVLLRECL"
     gene            complement(2905..5535)
                     /gene="dnaE"
                     /locus_tag="VF240_04665"
     CDS             complement(2905..5535)
                     /gene="dnaE"
                     /locus_tag="VF240_04665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7174560.1"
                     /translation="MSANDFAHLHLHTDYSLLDGAIQIKPLAERVRELGMKACAMTDH
                     GNMYGAVSFYKEMKAHDIKPLIGCEIYLAPGSRFDKADARKGEKPYNHMILLAKDREG
                     YHNLVRLTSRAFKEGFYRKPRIDRELLAQFSGGIIGLSACLSGVPQARLKEGKFDEAA
                     RAAQEFQDILGKGNYFLEIQDHQLPDQQKINKDLIELSKRTAIPLVATNDAHYLYRDD
                     YEAHDVMLCIGTGRTVNDTDRMRFGGPQWYVRSAQEMREIFTYCPDAVSRTMEVAEMC
                     DLKLPLGENRLPVFPIPPEEGDITIDEYFERVIRLGYEKRRQTVWDAQAGAGKLKHSL
                     GEYQERVSREIAMIKQMGFPSYFLIVWDFIKFAKDRGIPVGPGRGSAAGSLIAYCLEI
                     TDVDPLQYDLLFERFLNPERVSMPDIDIDFCVHGRAEVINHVTQLYGRESVCQIITFG
                     TMASKAAIKDVGRALDMPYADVEKIAKLIPPPIRGRNVSIDQALETVPELKQAFETEP
                     QTKKVIDIARRLEGCARHSSVHAAGVVISPQPLEELIPVAVNQKQELTTQFEMSDLEK
                     TGMLKMDFLGLTTLSIISQCCKTIRDTTGRDICWSEVALDDLKTYDIFARGHTEAIFQ
                     FESTGMQEICRKLRPKSIEDLSALNALYRPGPLDGGMVDDYIDRYHGRKPIEYITPEM
                     EETLRNTYGILVYQEQIMQLAQRLGGYSLGEADLMRRAMGKKKREEMAEHEEKFVTGC
                     VKNKIAKKKAKEIFRLMAQFADYGFNRCLAGNSEVVDADTGALVSIKEIAEGHADIAR
                     TFSFDGERVFVNEIVEAFETGEQEVVEFETEDGQKVRCTLDHKFYTDEGYLPLREIIE
                     KGLELNFSPEVEEYATCRVE"
     gene            complement(5932..6099)
                     /locus_tag="VF240_04670"
     CDS             complement(5932..6099)
                     /locus_tag="VF240_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174561.1"
                     /translation="MKRHATLALALALPQTACIVGGYSSEGGFFLWPGSLILTAIIVA
                     VLFLLMRRRRR"
     gene            6465..9944
                     /locus_tag="VF240_04675"
     CDS             6465..9944
                     /locus_tag="VF240_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7174562.1"
                     /translation="MKRTVLLVALLMFCGVVSASAQTESPLLPRNPSISREQIAFVYA
                     GDLWVVGREGGDARRLTAGVGTETNPMFSPDGRLIAFTADYEGNTDVYVVEAAGGVPR
                     RLTYHPGVDAVAGWTPDGASVLFRTQRDSYSFAPRLFTMPLAGSNPVEVPLPQAAEGS
                     FSPDGRRMAYVPLVRAFQQWKGYRGGRTTPVLVADLSDSSVEKLPRENSNDFNPMWVG
                     ERVYFLSDRDGAVTIYSYDTRTKRVAQAVRNTGLDIKSAAAGDGVIVYEQFGSLHTLD
                     LSSGKTRQIPVRVAGDVSAVRPKFVRVAAGIRNIGLSPSGARAVFEARGEILTVPAEK
                     GDVRNLTNNSAAAERDPAWSPDGKWIAYFSDESGEYALHLRAQSGMGEVRKINLGSPA
                     SYFYSPVWSPDSKKIAYTDKRLNLWYVNVEQSGVAPVKVDTNTYDNPFRVMDPAWSPD
                     SRFITYTKQLKNRLGTVFIYSLEEQKTRQVTDNMSDARFAQFDRDGKHLYFTASTNSG
                     PTSGWLDMSSFGRQVTRSVYVVVLGKDSPSPLAPESDEEKILEEQKTNNPEEKGDPRP
                     GPSPSPTPVMGAISTDDAPAQADDEANISAVEQQTQEPAGDKPAAGRGGAAKKPEPVR
                     IDFEGIDQRVLALPIPPRDYAGMRAGKAGTLFLLENAPGPTDTFGLVLQRFDLSKRKV
                     EKVTEGLTSFDLSANGEKMLFQQGGGQGAPPAAAAPGQQQGGRWFIVSATQPLKPGEG
                     LINTAAMEVRVDPRAEWRQMYREAWRIQRDFFYDPTYHGLDIAATEKKYEPFLSGLAH
                     RADLNYLLQESLGNMVVGHHNTGGGDTPQPTPVPGGLLGADYKVEQGRYRFARIFNGE
                     NWNPSLRAPLTQPGVNVRVGEYLLAVNGRELRAADNLYSFFEGTANKQTVIRVGPNAD
                     GSGARETTVVPVASESGLRNLAWIEGNRRRVSEMTGGRVAYVYLPNTSTAGYTNFNRY
                     YFSQIDREAAVIDERFNGGGTAADYMIDYMRRPLMNYWATREGADFTTPVGSIYGPKV
                     MLIDEYAGSGGDALPWYFRRAKIGPLVGKRTWGGLVGIYDYPQLIDGGSVTAPRVAFY
                     SPEGEWEVENAGVAPDIEVELDPKAWRQGRDLQLEKAIEVVMEALRKNPPPAPRKPVY
                     PNYHNGRPTVSRQ"
     gene            complement(10066..10138)
                     /locus_tag="VF240_04680"
     tRNA            complement(10066..10138)
                     /locus_tag="VF240_04680"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10103..10105),aa:Ala,seq:ggc)
     gene            complement(10242..11024)
                     /locus_tag="VF240_04685"
     CDS             complement(10242..11024)
                     /locus_tag="VF240_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7174563.1"
                     /translation="MASYETILLERRGGVALLTINRPEKRNALNIQTRQEGAAALDEL
                     REDERVRVVVVTGAGDKAFIAGADISEFEGRTANTQRDVMLGRSLFTAVDTFTKPLIA
                     MVNGYCLGGGCELAMACDIRIASDKASFGQPEINLGIIPGGGGTQRLTRLVGEGKAME
                     LILTGDIIDAQTAYNIGLVQMVVPAPDLEAKTMELAGRIAEKSPVALRLAKEAVRIAS
                     RSNLDEGLRREVDLFALCFSSEDKDEGVRAFLEKRKPQFKGR"
     gene            complement(11128..11520)
                     /locus_tag="VF240_04690"
     CDS             complement(11128..11520)
                     /locus_tag="VF240_04690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7174564.1"
                     /translation="MDKATPNIEKREERRFALRMALRLPIVVSGRSEDGAAWSEPTET
                     DDISTMGTLFHLNQRVGRGDQLYIRAHKPDGSPIEVTAKVIRIAPAIYGTARIGVQIV
                     EPADNWLRLFVSWVADEQGTAAEGEQSQ"
     gene            complement(11619..12518)
                     /locus_tag="VF240_04695"
     CDS             complement(11619..12518)
                     /locus_tag="VF240_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7174565.1"
                     /translation="MVTPENEKSDETSPPLAVERISVLGAGTMGHGIAQVAAMSGYRV
                     TLRDISDDVTLRALRAIDANLSKGVERGKVTEEERETALANIRVTTDMGETALADLFV
                     EAVPERMELKREALREVERVAAGDFVFATNTSSLSITEIARGARRPERVVGMHFFNPV
                     HIMRLLEVVVGRETSAETCETARAVGRRMRKEPITVRDVPGFASSRLGVALGLEAMRM
                     VEEGVASAKDIDTAMELGYNHPMGPLKLTDLVGLDVRLQIAEYLYGEFGAEGFRPPDI
                     LRRMVAAGKLGKKSGEGFYEWKE"
     gene            12652..13812
                     /gene="trxB"
                     /locus_tag="VF240_04700"
     CDS             12652..13812
                     /gene="trxB"
                     /locus_tag="VF240_04700"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7174566.1"
                     /translation="MGTRRTSALDCVRRAPPRRVSNIMEQETIHKEVVIIGSGPAGLT
                     AAIYAARADLKPLVVHGPQPGGQLTITTDVENYPGFAEGIQGPALMEEFEKQARRFGT
                     EFLQTFISKVDLSERPFKLYTDDHLIEAETLIIASGASAKWLGIPGEAPYPEGLGGFG
                     VSACATCDGFFFKGRTIVVAGGGDTAMEEATFLTRYASRVTVIHRRDKLRASQIMQQK
                     ALANDKIDFLWDTEIREVLGSKDTGVTGLRIFNKKTGEERVFPCEGFFVAIGHKPNTD
                     IFRGWLELDEVGYIKTAGRSMATNIPGVFACGDAQDSFYRQAVTAAGTGCMAAIDAER
                     YLDNLPVVTPTGEEINMDGDRVMPSHDIVIMADGKVMSNKPDDVPGEATAGD"
BASE COUNT         2505 a         4586 c         4456 g         2527 t
ORIGIN      
        1 gagttgggga agtcggacga cgtgcgcctg acggcgcgtc tgatgccgca ggtgggttgg
       61 gtgatgcgcc acgcggcggg cacgggcgcg gcgacgccgc tcggcctcaa actcgtggac
      121 tacttccgcg agcgcggcta cgaggtgact tacgtgggcg gcgagcaagg cgctatggcg
      181 acggagaagt acgcggtcga gcgggccatg tacgagctgc gctggcaggg ggcgaacgtc
      241 gtggctctcg cgcccggccg agtagtcgcc ttcgagcaca acgtccacac caacgaggcg
      301 ctgcgccgcg cgggcgtgga cgttgtaacc ttccccggcg aattgctctc gatccgcaat
      361 ggcgggccgc actgtctcgt gatgccgctc gttcgcggcg actgacggcg acccgccgtc
      421 gcattcacag cgtgttgatt taaagcaaca cccagccgcg gagcggcggc agaatgtagc
      481 ccacggcgtg agccgtggga acacagacac gataaagatt tgaaagcccc ggaggggcgg
      541 aagaacttca agcctgagca taaagctcgt cgaggttctt tcgcccctcc ggggctttcg
      601 atttttattt gcgtctattc cacgggctga cgcccgtggc tacattcttc cgccgctaac
      661 agcggctcgt tgtcgcgttc ctctatcggg cttgcttgtg cgacgtgctg cggttcgtcg
      721 aaatcagttc atcgagctgg cgtcggccca gcggatgcgc gcgccggtgg aggcgagggc
      781 gtcctggagc ttctggctgg ggaagacttt gatgagacgg ttggtgcggg cgcggacgag
      841 gaggccgtcg ggcaggttca tctcgatgaa gacttcgcag tcgccgggat tggagacgag
      901 cgcctgcgtg acgctgtcgc acagcgtcgc gaggtcggcc gacgccgccg gcatctcgat
      961 gaccagcgcc ttgcccgcgt tgcggccgtt gcgaaaaccc gcggccggga cgtgcgccgg
     1021 cacgcgcgcc ttctctaaaa gcatcacctc gtcgcacacc agcgtcacgg agccttcgcc
     1081 gctgccgtcg atacgcccga cggcgaggac gacgcggtcg tcggccgcat cgccgccctt
     1141 catcttcaaa gcctccggcc agaggacgca cttgacggag acgcccgtca tgtcttcgag
     1201 acggaagaag gcgtagcggt cgcccttctt ggtgttgcgg acggtgaagt cggagacgac
     1261 gccggccagg cgcaggcgcg cgtccggcgc agcgcccgag aggtcggcga ccgacgcgca
     1321 gccgagttgt tcgatgcgct cggcaaaatc ctgcatcggg tggcccgaga cgtagaagcc
     1381 gacggccgcc ttctcacgcg tcagcaagtc cttcggcgcc cacgcctcgg ccgccgcgaa
     1441 catgtgcgtc ggcgcgttcg actcttcagc cgcgccgccc gcgacgaagc cgaacatggc
     1501 gacctgcccg ctgtcgcgct cgcgccgcgt gcgcgagccg ccctccagcg ctcgatccac
     1561 tgacgcgaac agacacgccc gccagtggtg caaagtctgc gccgccggct tcatcgagtc
     1621 gaacgccccg gcacacacca gactctccag cacgcgcttg cccgaaccct tctccccgac
     1681 gcgctcctcg aagtcgaaga tcgacgtgta aggccgttcc gcgcgccccg cgctgatggc
     1741 gccgaccgcc gactgaccca agcccttgat ggccgacagc ccgtaacgaa tcgcgccctc
     1801 gacgggcgtg aaaccagcgc ccgactcgtt cacgtcgggc ggcagcatct ttatcccttg
     1861 cgcgcgcagc tccgaggcgt acttgaaaat cttcgccgcg tcgtccgtac agtaagtcat
     1921 cacggccgcg tagaaatgct ctggatagtt ggccttcaga tacgcggtct ggtaagcgac
     1981 gtgtgcgtag gcgaccgagt ggctgttcgc cgtcaggatg ccgttgacga aatagttgtg
     2041 gtgcggcccc cgcatggtca tgttgtaggt cttttcaacc ccaaccgatg tcacgcgcac
     2101 gatttttgtc ttcttcactc tcataaacac tctcgaagca gcactaactt cctctccaaa
     2161 tactgctggg cctcgcggta aaggcaccgg gctatctgcc gcacgtccga tttgtgcgac
     2221 cactggacgt agcgaatcgc gccgttctca tggacacgat tgtccgagac accgagacgg
     2281 cagataagcc acatccgcac ctcggcaacc agccgctcgc tcccgcaaaa gccaacaatg
     2341 acctcgccgt cagttcgcct gctcacagtc ccatccccgt cgaaaattcc gcgtatcagg
     2401 tgaggcatca agtcctcggc cagaatcggc agatatgtgc agaggctctt tctcggtatg
     2461 acgccgtgcc gtgctaaatc acttgccatg cgtcgcgagt ggcagatcac gcgatacatc
     2521 ggtcggtgac gctcgcccct cggcgcgata cgaagcacgg ggttcttaag gccgaggaaa
     2581 ttcctaaaac cttcaaccgc ctcgcggtct gtcatttgca gtcctacctg cggagttcgt
     2641 cccggtcctc cgtctatcac gtagccgtcg ctgattagca ggccgagcca gtaagctttg
     2701 gccggagaat caatcacggc gaaggccgcc tcgtcgcagg ttttgtagtc tgcgatcccg
     2761 caccgcttcg ggacgccgtg cttttcgaga acgtcgtaga cagtcttcct cgtcttaaac
     2821 gggatagatt tcaaaatctc gcccgcgctc aagccgccct tatacttctc gacaatgaaa
     2881 tgctcgacct ccggggtcaa ccttttattc cacgcggcat gtggcatact cttcaacctc
     2941 tggcgagaaa ttaagctcaa gccccttctc gatgatctcc ctgaggggca gataaccttc
     3001 gtccgtatag aatttatgat cgagagtgca gcgcactttt tgcccgtctt ccgtttcgaa
     3061 ctcgactact tcctgttccc ccgtctcgaa cgcttcgact atctcgttga cgaacacccg
     3121 ctcgccatcg aagctaaatg ttcgtgcgat gtcagcatgc ccttcggcta tctctttgat
     3181 tgaaaccagc gcgcccgtgt ctgcatcaac cacttccgaa ttgcccgcca aacatcgatt
     3241 gaaaccatag tcggcgaact gcgccatgag gcggaagatt tctttggcct tcttcttggc
     3301 gatcttgttc ttcacgcagc cggtgacgaa cttctcctcg tgctcggcca tctcctcgcg
     3361 cttcttcttg cccatcgcgc ggcgcatgag gtcggcctca cccaagctat agccgccgag
     3421 acgctgcgcg agctgcatga tctgttcctg atagacgagg atgccgtagg tgttgcgaag
     3481 agtctcctcc atctccggcg tgatgtactc gatgggcttg cggccgtggt agcggtcgat
     3541 gtagtcgtcc accatgccgc cgtccaaagg ccccggccgg tagagcgcgt tcaaagccga
     3601 caggtcttca atcgacttcg gccgcagctt ccggcatatc tcctgcattc ccgttgactc
     3661 aaattgaaat attgcttctg tatgaccacg cgcgaaaatg tcgtaagtct tgaggtcatc
     3721 gagcgcgact tccgaccagc agatgtcgcg ccccgtggtg tcgcggatgg tcttgcagca
     3781 ctggctgatg atggagaggg tggtcaggcc gaggaagtcc atcttgagca tgcccgtctt
     3841 ctcaaggtcg gacatctcga actgggtcgt caactcctgc ttctggttga cggcgacggg
     3901 gattagttct tcgagcggct gcgggctgat gacgacgccg gcggcgtgca ccgaagagtg
     3961 gcgggcgcag ccttcgaggc gtcgggcgat gtcgatgacc tttttagtct gcggctcggt
     4021 ctcgaaggcc tgtttgagtt cgggcacggt ttcgagcgcc tgatcgatgg agacgttgcg
     4081 gccgcggatg ggcggcggga tgagcttggc gatcttctcc acgtcggcgt agggcatgtc
     4141 gagcgcccgg cccacgtcct tgatggcggc cttcgaggcc atcgtgccga aggtgatgat
     4201 ctggcagacc gactctcggc cgtagagctg tgtgacgtgg ttgatgactt cggcgcggcc
     4261 gtggacgcag aagtcgatgt cgatgtcggg catcgacacg cgctcgggat tcaggaagcg
     4321 ctcgaagagc aggtcatact ggagcgggtc aacgtccgta atttcgagac agtaggcgat
     4381 gagcgaacct gcggccgatc cacggcctgg gccgacgggg atgccgcggt ctttggcgaa
     4441 cttgatgaag tcccagacga tgaggaagta ggaggggaag cccatctgct tgatcatcgc
     4501 gatctcgcgc gagacccgct cctgatactc gccgagggag tgcttgagct tgccggctcc
     4561 ggcctgagcg tcccagacgg tctggcgtcg cttctcgtag cccagccgga tgactcgctc
     4621 gaaatactcg tcaatcgtga tgtcgccttc ctcgggcggg atgggaaaga cgggcaggcg
     4681 gttctcaccc agcgggagct tgaggtcgca catctcggcg acttccatcg tgcgcgagac
     4741 ggcgtcgggg cagtaggtga aaatctcgcg catctcctga gcggagcgga cgtaccactg
     4801 cgggccgccg aagcgcatgc ggtcggtgtc gttgacggtg cggcccgtgc cgatgcagag
     4861 catcacgtcg tgggcctcgt agtcgtcgcg gtagaggtag tgtgcgtcgt tggtggcgac
     4921 gagcgggatg gcggtgcgct tcgacagctc gatcaggtct ttgttgatct tctgctggtc
     4981 ggggagctgg tggtcctgaa tttcgaggaa gtagtttccc ttaccgagta tgtcctgaaa
     5041 ctcctgggcg gcgcgggcag cctcgtcgaa cttgccctct ttcagacgcg cctgcgggac
     5101 gccggacaga cacgcggaga gaccgatgat gccgccggag aactgtgcga gcagctcgcg
     5161 gtcgatgcgc ggcttgcggt agaagccctc cttgaaggcg cgcgaggtga ggcgcacgag
     5221 gttgtggtag ccctctcgat ccttcgccag caggatcatg tggttgtagg gcttttcgcc
     5281 cttgcgcgcg tcggccttgt cgaagcggct gccgggggcc aaatagattt cgcagccaat
     5341 cagcggcttg atgtcgtgcg ccttcatctc cttgtagaag gagacggcgc cgtacatgtt
     5401 gccgtgatcc gtcatggcgc acgccttcat gccgagttcg cgcacgcgtt cggcaagggg
     5461 cttgatctgg atggcgccgt cgaggaggct ataatctgtg tgtaagtgca ggtgggcgaa
     5521 gtcgtttgct gacatgctga aaaatcgtac ccgtgcggct cggttttatt tatcgcgggg
     5581 cgtgcggcgc gccccacgcg tggaatggcg atgtgttaat aggccatttc gcgcgacgtg
     5641 tcaacgggcg tcttctaagc gccggaaaag ccgggaatta agtgggccgg gtggggcttc
     5701 ggggcgggag ggttagacag gacttacagg attttcagga ttgacaggat gatgaaagag
     5761 ctgatttgaa atttcagatg tcagatttga gatttcaaat cgccccgtcc tgtcagtcct
     5821 gcaaatcctg taagtcctgt ccatccatgt taattgctct ttcggaaacg cgggtcgcgg
     5881 actcgccccg cccctacaga ggtgaccggc gtcgaagtct gaaaccgact cttaacgccg
     5941 ccgccggcgc atgagcagga agaggacggc gacgatgatg gccgtcagta tcaggctgcc
     6001 gggccagaga aagaagccgc cctcgctgct gtagccgccg acgatgcagg ccgtctgcgg
     6061 cagagcgagc gcgagcgcga gtgttgcatg tcgtttcatg agctaccccc ttaaattgct
     6121 gtgccgcagc gcccgactcc ggccgccgcc gacagtttaa cgaacaagcg gcgtcgaggc
     6181 taacggacgg acacgcccac cgcccaccgc acggagttgc ggattgacac gccgcggagc
     6241 gttggcatag attacccgtc cgttcattaa ttacgggctg acccgacgag tcttgcgccg
     6301 cctcgcgtct cctcagcttc agccttcgag actgtcttaa cgcccactcg acgctcgccc
     6361 acgcgccgtg cgacgtgtgg ggaaccgccg ccgtcacggc ttccgccgaa gcttccgccc
     6421 gaacgagaaa atcgagtccg acaaccaaca ccggagtagc cgaaatgaag agaaccgtcc
     6481 tccttgtcgc cctgttgatg ttttgtggag tggtcagcgc aagcgcgcag acggagtcgc
     6541 cgctgctccc gcgcaacccc tcgatcagcc gcgagcagat cgcgttcgtc tacgccggcg
     6601 acctgtgggt cgtcgggcgc gagggcggcg acgcgcggcg tctgacggcc ggcgtcggca
     6661 ccgagacgaa ccccatgttc tcgcccgacg gacggctcat cgccttcacg gccgattacg
     6721 aggggaacac ggatgtctac gtcgtcgagg cggcgggcgg cgtgccgcgc cgcctgactt
     6781 atcaccccgg agtggacgcc gttgccggct ggacgcccga cggcgcgagc gttctcttcc
     6841 gcacgcagcg cgacagctac tcgtttgccc cgcgtctgtt caccatgccg ctcgccggca
     6901 gtaaccccgt cgaagtgcct ctgccgcagg ccgccgaggg cagcttctcg cccgatggac
     6961 ggcgcatggc ttacgtcccg ctcgtgcgcg ccttccagca gtggaagggc tatcgcggcg
     7021 ggcgcaccac cccggttctc gtcgccgacc tctcggattc gagcgtcgag aaactgccgc
     7081 gcgagaactc gaacgacttt aacccgatgt gggtcgggga gcgcgtctac ttcctctccg
     7141 accgcgacgg cgcggtgacg atctattcct acgacacgcg cacgaagcgc gtcgcgcagg
     7201 ccgtcaggaa cacgggactc gacatcaagt cggccgcggc cggcgacggc gtgatcgtct
     7261 acgagcagtt cggctcgctc cacacgctcg acctttcgtc gggaaagacg cggcagattc
     7321 cggtgcgcgt cgcgggcgac gtgtcggccg tgcgccctaa gttcgtgcgg gtggccgcgg
     7381 ggatacgcaa catcggtctg tcgccctcgg gcgcgcgggc cgtcttcgag gcgcgcggcg
     7441 aaatcctgac cgtgccggcc gagaaaggcg acgtgcgaaa tctgacgaac aactccgcgg
     7501 ccgccgagcg cgaccccgca tggtcgcccg acggcaagtg gatcgcctac ttctccgacg
     7561 agtcgggcga gtacgccctg cacctgcgcg cgcagagcgg catgggcgag gtgcggaaaa
     7621 tcaacctcgg cagtccggcc tcgtacttct actcgcccgt ctggtcgccc gactcgaaga
     7681 agatcgccta cacggacaag cgtctgaacc tgtggtatgt gaacgtcgag cagtcgggcg
     7741 tcgcgcccgt caaggtggac acgaacacct acgacaaccc gttccgcgtg atggacccgg
     7801 catggtcgcc cgactcgcgc ttcatcacct acaccaaaca gctcaagaac cgcctcggca
     7861 ccgtctttat ctattcgctc gaagagcaga agacccgaca ggtgaccgac aacatgtcgg
     7921 acgcgcgctt cgcgcagttc gacagagacg gcaagcacct ttacttcacc gcgagcacga
     7981 actcggggcc gacctcgggc tggctcgaca tgtcgagctt cggccggcag gtgacgcgct
     8041 cggtctacgt cgtcgtcctc ggcaaagact cgcccagccc gctcgcgccc gagagcgacg
     8101 aggagaagat tctcgaagag cagaagacca acaaccccga ggagaagggc gacccgcgcc
     8161 ccggcccctc gcccagcccc acgcccgtca tgggcgccat ctccactgac gacgcgcccg
     8221 cccaggcgga cgacgaggca aacatctcgg ccgtcgagca gcagacgcag gagccggccg
     8281 gcgataaacc cgccgccggg cgcgggggcg cggccaaaaa gcccgaaccc gtccgcatcg
     8341 acttcgaggg aatagaccag cgcgtgctgg cgctccccat cccgccgcgc gactacgccg
     8401 gcatgcgcgc cggcaaggcc ggcacactct tcctgctcga gaacgcgccc ggccccaccg
     8461 acacgttcgg cctcgtcctc caacgcttcg acctctcgaa gcgcaaggtg gagaaggtta
     8521 ccgaagggct gacctccttc gacctctcgg ccaacggcga gaagatgctc ttccagcagg
     8581 gcggcggtca gggcgcgccg ccggctgccg ccgcgccggg tcagcagcag ggcgggcgct
     8641 ggttcatcgt ctcagccacg cagccgctca agccgggcga aggcttaatc aacacggccg
     8701 cgatggaggt gcgcgtcgat ccgcgcgccg agtggcggca gatgtaccgc gaggcgtggc
     8761 gcatccagcg cgacttcttc tacgacccga cctaccacgg cctcgacatc gcggccaccg
     8821 agaagaagta cgagccgttc ctgtcgggcc tcgcgcaccg cgccgacctc aactatctcc
     8881 tgcaagagtc gctcggcaac atggtcgtcg gccaccacaa cacgggcggc ggcgacacgc
     8941 ctcagccgac acccgtcccc ggagggctgc tcggcgccga ctacaaagtc gagcaggggc
     9001 gctaccgctt cgcgcgcatc ttcaacggcg agaactggaa cccctccttg cgcgcgccgc
     9061 tcacacagcc cggcgtcaac gtccgcgtcg gcgagtacct tttggccgtc aatggtcgcg
     9121 agctgcgcgc cgccgacaac ctctacagct tcttcgaggg cacggccaac aagcagaccg
     9181 tcatccgcgt cggccccaac gcggacggct ccggcgcgcg cgagacgacg gtcgtccccg
     9241 tcgcgagcga gtcgggcctg cgcaacctcg cgtggatcga gggcaaccgc cggcgggtct
     9301 cggagatgac tgggggccgg gtcgcctacg tctacctgcc gaacacctcg acggccggct
     9361 acaccaactt caaccgctac tacttctcgc agatcgaccg cgaggcggcc gtcatcgacg
     9421 agcgcttcaa cggcggcggc accgcggccg actacatgat cgactacatg cgccggccgc
     9481 tcatgaacta ctgggcgacg cgcgaggggg ccgacttcac cacgcccgtc ggctccatct
     9541 acggcccgaa ggtgatgctc atcgacgagt acgccggctc gggcggcgac gccctaccgt
     9601 ggtatttccg ccgtgccaaa atcggccccc tcgtcggcaa gcgcacatgg ggcggactgg
     9661 tcggcatcta cgactacccg cagctcatcg acggcggctc cgtcaccgcg ccccgcgtcg
     9721 ccttctacag ccccgagggc gaatgggaag tagagaacgc cggcgtcgcc cccgacatcg
     9781 aagtcgaact cgacccgaag gcgtggcgtc agggtcgcga cctgcaactc gaaaaggcca
     9841 tcgaggtcgt catggaggcg cttcgcaaga acccgccgcc cgcgccccgc aaacccgtct
     9901 acccgaacta tcacaacggc cggcccacag tgtcgcggca gtaaaaaaaa cagtaccgcg
     9961 agcggtagcg agcgggtgtc gctcccgaga gcagagccgc tcgcggtact gtcattaccc
    10021 aaaaactaag ggcagcccgt gtggctgccc tttcacttct ctaaatggag ccgaggagga
    10081 tcgaactcct gacctcttga atgccattca agcgctctcc cagctgagct acggcccctc
    10141 ggaacaagtc gcccttgcgg ggcggtaaaa agagtagcgc gaggcgagaa aggcagtcaa
    10201 gcgcattgag cggcggcgcg tgcgagcttc atggcttgcg gctacctgcc tttgaactgc
    10261 ggcttgcgct tctctaagaa ggcgcggacg ccctcgtcct tatcttcgct ggagaagcag
    10321 agggcgaaga gatcgacctc gcggcgcagg ccctcgtcga ggttcgagcg cgaggcgatg
    10381 cggacggcct ctttggcgag gcgcagggcg acgggactct tctcggcgat gcggccggcc
    10441 agttccatcg ttttggcttc gaggtccggg gcgggcacga ccatctggac gaggccgatg
    10501 ttgtaggccg tctgcgcgtc gatgatgtcg cccgtgagga tcagctccat cgccttgcct
    10561 tcgccgacga gacgcgtgag ccgctgtgtg ccgccgccgc cggggatgat gccgaggttg
    10621 atctcgggct ggccgaaaga agccttgtcg ctggcgatgc ggatgtcgca ggccatggcg
    10681 agttcacacc cccccccgag gcagtagccg ttgaccatgg cgatgagtgg tttggtgaac
    10741 gtgtccacgg cggtgaagag cgagcggccg agcatcacgt cgcgctgcgt gttggccgtg
    10801 cgcccctcga actcgctgat gtcggccccg gcgatgaacg ccttgtcgcc cgcgcccgtg
    10861 acgacgacga cgcgcacccg ttcgtcctcg cgcagctcat cgagcgccgc cgcgccctcc
    10921 tgacgggttt ggatgttgag ggcgttccgc ttctcgggcc ggttgatggt caaaagcgcg
    10981 acgccgccgc gccgctcaag caggatggtc tcgtaggatg ccattggatt ttttgctccg
    11041 caaaaaaagt gataagtgat gagtgataag tgataagacg aagagcaacc gctctgtctt
    11101 ttcttatcac ttatcactca tcacttatca ctgactctgt tcgccctcgg ccgcggtgcc
    11161 ctgttcgtcg gcgacccagg agacgaagag acgcagccag ttgtcggccg gctcgacgat
    11221 ctggacgccg atgcgcgccg tgccatagat ggccggggcg atgcggatga ccttggccgt
    11281 gacctcgatg ggcgagccgt cgggcttgtg cgcgcggatg tagagctggt cgccgcggcc
    11341 cacgcgctgg ttgaggtgaa agagcgtgcc catcgtcgag atgtcgtcgg tctcggtcgg
    11401 ctcgctccac gccgcgccgt cttccgagcg gcccgagacc acgatgggca gccgcagcgc
    11461 catgcggagc gcaaaccgcc gctcttcgcg cttctcgatg ttgggggtcg ccttgtccat
    11521 aagtcaggag tcaggagtca ggagtctcag gagtcagaat gagaagcaat caggcaggga
    11581 gcgaatcaat tctgacttct ggctcctgac tcctgacttc attctttcca ctcgtagaag
    11641 ccttcgccgc tcttcttgcc cagtttgccc gccgcgacca tgcgccgcag gatgtcgggc
    11701 gggcggaagc cttcggcgcc gaactcgccg tagaggtact cggcgatttg aagacgcacg
    11761 tcgaggccga ccagatcggt taatttcagc ggccccatgg ggtgattgta gcctagttcc
    11821 atcgccgtat ctatatcttt cgcactggct acgccctcct cgaccatccg catcgcctcc
    11881 agccccagcg ccacgcccag ccggctcgaa gcgaagccgg gcacgtctct gaccgtgatc
    11941 ggctccttcc gcatccgccg ccccacggcg cgcgccgtct cgcatgtctc ggcggaagtc
    12001 tcgcggccga cgacgacttc gagcaggcgc atgatgtgga cggggttgaa aaagtgcatc
    12061 ccgacgacac gctccgggcg acgtgcgccg cgcgcgatct ccgtgatcga cagagaggaa
    12121 gtgttggtgg cgaagacgaa gtcgcccgcg gccacgcgct ccacctccct gagcgcctcg
    12181 cgcttcagct ccatccgctc gggcacggcc tcgacgaaga ggtcggccag ggccgtctcc
    12241 cccatgtcgg tcgtcacccg gatgttcgcc agcgccgtct cgcgctcctc ctcagtcacc
    12301 ttgccgcgct cgacgccctt cgagaggttc gcgtcgatgg cgcgcaaggc gcggagcgtc
    12361 acgtcgtccg agatgtcgcg cagagtcacc cggtagccgc tcatcgcggc gacctgcgcg
    12421 atgccgtgtc ccatcgtccc ggccccgagc acgctgatgc gctcgacggc gaggggcggc
    12481 gaggtctcgt cagatttttc gttttccggt gtgaccaaaa cggtgaaagt ttgcaggggc
    12541 ctcgaccgag ccggggccga ctctctgaag ccaccccttc gggcgcggga ggcatattcg
    12601 actccgactc gcgcgagacg ttatactaca cgcgcaaggc aaaaggctac tgtgggcacc
    12661 agacgcacga gcgccctcga ctgcgtgcga cgcgcgcccc cgaggagagt gtcgaacatc
    12721 atggagcagg aaacgattca caaggaagtc gtcatcatcg ggtcggggcc ggcggggctg
    12781 acggccgcca tctacgcggc gcgcgcagac ctcaagccgc tcgtcgtcca cggcccgcag
    12841 cccggcggcc agctcaccat cacgaccgac gtggagaact accccggctt cgccgagggc
    12901 attcaggggc cggcgctgat ggaggagttc gagaagcagg cccggcgctt cggcaccgag
    12961 tttctgcaaa cgttcatctc caaagtcgat ctctccgagc gcccgttcaa gctctacacg
    13021 gacgatcatc tgatcgaggc cgagacgctg attatcgcct cgggcgcctc cgccaagtgg
    13081 ctcggcattc cgggcgaagc gccgtacccc gagggcttgg gcggcttcgg cgtctcggcc
    13141 tgcgcgacgt gtgacggctt cttcttcaag ggccgcacaa tcgtcgtcgc cggcggcggc
    13201 gacacggcga tggaggaggc cacattcctc acgcgctacg cctcgcgcgt caccgtcatc
    13261 caccgccgcg acaagttgcg cgcctcgcag atcatgcagc agaaggcgct cgccaacgac
    13321 aagattgatt tcctgtggga cacggaaatc cgcgaggtgc tcggctcgaa ggacacaggg
    13381 gtgacgggcc tgcgaatctt caacaagaag acgggcgagg agcgggtctt cccctgcgag
    13441 ggcttcttcg tcgccatcgg ccacaagccg aacacagaca tcttccgcgg ctggctggaa
    13501 ctcgacgagg tcggctacat caagacggcc gggcgctcga tggccacgaa catccccggc
    13561 gtcttcgcct gcggcgacgc gcaggactcg ttctaccggc aggccgtcac cgccgccgga
    13621 acggggtgca tggccgccat cgacgccgaa cgctacctcg acaacctgcc cgtcgtgacg
    13681 cccaccggcg aggagatcaa catggacggc gaccgcgtca tgcccagcca cgatatcgtc
    13741 atcatggccg acggcaaggt catgtcgaac aagccggacg acgtgcccgg cgaggcgacg
    13801 gccggagact aagcgggacg gcgtcgatca aaaccgcggc ccgaagtttt ttctcggacg
    13861 agcacagcgg cgaagccccg gcaggggcgg aagaatgtag cccacggcgt cgggccgtgg
    13921 gtcagcgtcg cttaaaaaca cggaagcccc gtcaggggcg aaagaaccgc gacgagattc
    13981 gtgctcaacg tcgcggttct ttcgcccctg acggggcttt cagactttgt tatcacgtcc
    14041 gttcccacgg ctcacgccgt gggctacatt cttc
//
LOCUS       DASVES010000130        11256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_223302, whole genome shotgun sequence.
ACCESSION   DASVES010000130 DASVES010000000
VERSION     DASVES010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223302"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..1169)
                     /locus_tag="VF240_04705"
     CDS             complement(351..1169)
                     /locus_tag="VF240_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174567.1"
                     /translation="MLAAALSSVASGAHAQTAAPVEKKKDEKKAEKTSDAGLTAEGIA
                     EGVLLVFGGRAQLEQVRRTGVERGRISRTATDGTPVEIEYERSFKRGETSDKDKIRVD
                     QRRTNLEYSLIFNDGRLWGIIKGTSFAPRQEDVSDFDSARQHGIENLLRYKENGATLN
                     YVGKDKQKNIDMWVLDLTEKAGGRTRYYISSQTGKVLWLEYETAQQPGGTPVKYRRTF
                     HDYRIVQGTRVPYRSVLYANDKMAEEAQILTVTYGVKMEDSRFKSEEASNTNGN"
     gene            1533..2768
                     /locus_tag="VF240_04710"
     CDS             1533..2768
                     /locus_tag="VF240_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174568.1"
                     /translation="MKRVGLLVGREKSFPQALIEAINERGAGEVTAEFITVGGIRHDY
                     EKQYDLIVDRISHEVPFYRAFLKRAALEGTVIVNNPFWWSADDKFFNYSLAEKLGVAV
                     PRTVLLPQKDYIEGIVSDSLRNLEFPLDWQGITDYVGFPAFIKPFDGGGWKNVSKVNS
                     LEELWRVYDTTGTLCMTLQQGIEYDQFVRCYCIGQEDVMIMPYDPSKPYLSGEQYLHN
                     PTYLSTELAQRVTDDVRKLCRALGYDINTCEFAIKDGVPYAIDFMNPAPDADLHSVGE
                     FYHNWVTKAVTDLVFKRLAEPQPAPRYRWDALLNPVPEPAGAREALSAIATAAEQASR
                     TIIPQPVRDFTADVAAGVTDLVEQAAGAVSSLVGSDEVAEEPAAKDNKKPRSSAVKGK
                     TPAKKSAAPRGRKTPSTEV"
     gene            3146..3218
                     /locus_tag="VF240_04715"
     tRNA            3146..3218
                     /locus_tag="VF240_04715"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3179..3181,aa:Phe,seq:gaa)
     gene            complement(3308..5674)
                     /locus_tag="VF240_04720"
     CDS             complement(3308..5674)
                     /locus_tag="VF240_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174569.1"
                     /translation="MHHRYIKTRVGLLLSCALLFSSLLPPALSQTATQNPPAAQQDEV
                     LRISTNLVQTDLVVLDKQGRTVRGLTKDQFELSVEGQPQEISFFENVETGSAREAAQL
                     AAARESSGTPNNTPSQPRAAALGTSVGRTFIFFVDDMHLAPEGIKRARDLLNNFVDAM
                     GEDDQALLISPSGQIGFLQQLTGNKTALRLGISRLNYQSRSAPTQGRRAMTVYEALAI
                     DKGHRNVIDYKAKEYMDDMGFERQGGGASEQPQPTPPPPQQSGQPGGGGGGSGGGGGG
                     GGGGISSSVLRVPGTDMNMRRMTAETAVKNEARAVLFQSNAVGEAVLGSLEYVARGSA
                     TLPGRKLFFFISDGFILDTRSAQGTERLQRVIDTAARSGVAIYTVDSKGLTVSNVTAS
                     QDVFSDIGIGSIQGGGRERIDASTDASSIAMSADSATREILRTLAADTGGRAILNRND
                     LESGVRQVLTETESYYVLAWKPQVIESGKPKFSSLKVSIKGRSDLKVLSRKGFYHTPP
                     PPLASDNKTTAKDSKTPALKPSEADLRAAISSAFPRRQLGVSVYTTYANESSDSYKII
                     TFADLSNFQLAPGAGGKAAGEVDFAVAVLNDSGKSVTGVGQNVKISESSTQPFRIVAT
                     MPNALPAGLYQVRVAARDAQTGRVGSSFLWLEIPKFKAGELALSNILLAEVTGKAGEA
                     PALDVERRFARASSMLIQMFVYNAARSANGQADVAVTLQVLQNGKQLIASPPQPILTA
                     GVADQTRLQYGAQIPLSGFPPGRYTLKVTVNDRAAKTEASQQINITVE"
     gene            complement(6010..7335)
                     /locus_tag="VF240_04725"
     CDS             complement(6010..7335)
                     /locus_tag="VF240_04725"
                     /inference="COORDINATES: protein motif:HMM:NF005557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HEX7174570.1"
                     /translation="MLERSILIKGGTLVTVDAADSVVAADLLVRGSRIVAIGDDLGPA
                     DETIDARGCAVLPGFVQTHIHLCQTLFRGAADDLSLIDWLRKRVWPMEAAHSKQSVRA
                     SARLGIAEMIRGGTTCALTMETVRHTEEVFRVVEETGFRATVGKCMMDKGGDVPAPLR
                     EETEDSIGESVALLDAWHGRGGGRVRHCFAPRFAVSCTDALLRSVAALARERGVLIHT
                     HASENREEVEMVLAETGMRNVSYLSSVGLTGDDVVLAHCVHVDRDEQKLLAETGTHVA
                     HCPSSNLKLSSGIAPIIEMLARGVSVSLGADGAPCNNRLDMFTEMRTASLLQKVVGGA
                     EALPARRVLRMATADGARALGLAGEIGSLEAGKRADLILVDLERTHTTPRPDVVSTLV
                     YAAEAGDVRATLIDGEVVMKDGELKTLVERDVLSEARAHAEELFNHAKL"
     gene            complement(7530..8585)
                     /gene="recA"
                     /locus_tag="VF240_04730"
     CDS             complement(7530..8585)
                     /gene="recA"
                     /locus_tag="VF240_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX7174571.1"
                     /translation="MADSKINAERGKAVESALLNIEKKFGKGSIMRLGDREAADVLSI
                     STTSISLDAAIGIGGVPRGRVIEIYGPESSGKTTLALHIVAEAQKVGGVAAYIDAEHA
                     MDAEYAGKLGVKIDELLISQPDSGEQALEITEALIRSNGVDVIVIDSVAALVPRAELD
                     GEMGDSLPGLQARLMSQALRKLTAVVSSSNTSLIFINQIREKIGVMFGSPETTTGGRA
                     LKFYSSLRLDIRRIGAIKDGDRAVGNRTKVKVVKNKLAPPFRECEFDIMYGEGISREG
                     DVIDLGVANKVVEKSGAWFSYSGERLGQGRENSKQTLKDNPELLRRIETEVRKALGIA
                     GPDGEEPEAEAAATGKK"
     gene            complement(8939..9730)
                     /gene="trpA"
                     /locus_tag="VF240_04735"
     CDS             complement(8939..9730)
                     /gene="trpA"
                     /locus_tag="VF240_04735"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7174572.1"
                     /translation="MSRIAETFDRLRREGRRAFIPFVTAGDPDLQTTRALIPELARAG
                     ADIIELGIPFSDPVADGPVIQRASERALRGGVTVADVLAVVSEARRESDVPVVLFSYF
                     NPLMQYGVERLGRAAGDAGIDGVLVTDLVPEEAGEFAAVLDASGVNQIFLVAPTTTDA
                     RLSLVAGKASGFIYAVSRAGVTGARTEMSAEAEKLVGRVRRVSELPVAVGFGISSREQ
                     VAEVWRYADAAVIGSAIVAEMERLAGAPDLVSRVGAFVRTLVPEG"
     gene            complement(9727..10974)
                     /gene="trpB"
                     /locus_tag="VF240_04740"
     CDS             complement(9727..10974)
                     /gene="trpB"
                     /locus_tag="VF240_04740"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7174573.1"
                     /translation="MSETSTALPDSRGYWGKYGGRFVPETLMAPLEELTARYLEVRDD
                     EEFRARFESLLRHYSGRPTPLFYAARLTEHAGGARLFLKREDLSHTGSHKINNALGQA
                     LLAIRMGKRRVIAETGAGQHGVATATVCALFGLSCVVYMGAEDMRRQELNVFRMRLLG
                     AEVREVTTGSRTLKDAINEALRDWVSTPADTYYLLGSALGPHPYPLMVREFQSVIGRE
                     ARAQILETVGRLPDALVACVGGGSNSIGLFHPFLSDASVRMVGVEAGGRGGGLGEHAA
                     RFMPEGGGRPGVLQGTLSYVLQDDGGQIAATHSVSAGLDYPSIGPEHAHLRDTARVEY
                     VSASDSEALEAFRLLSRLEGIIPALESAHAIAHALRLAREMRSDQIVLVNLSGRGDKD
                     VQTVAALAEETAAVEVTSGEKSA"
BASE COUNT         2047 a         3523 c         3568 g         2118 t
ORIGIN      
        1 gttgtcgtac cagacggact ggccgagccg catgatttca cgcagtgggt tgttcatcga
       61 ctccgctcca aactcccgag gccagaaaga agtaggcaga gagacgcccg gcttcagtcg
      121 ccaacggtca acagaaacaa tttatttcgg cggatactaa cacagtgaaa aactctattc
      181 cagaacgctc acttgatatt aactcaaacg acgatacgag gaagtgtttt cgactccgac
      241 atcagtttga acaactcgga tgtcctccaa aagcaaaccg gctcaggcat gagcctgagc
      301 cggtttactc gtcctcctga tgtggcgcgc tcgcgggccg gggccgcgca ttaattgccg
      361 ttcgtgttcg acgcttcctc gctcttgaat ctcgaatcct ccatcttgac gccgtatgtg
      421 accgtgagga tttgggcctc ctccgccatc ttgtcgttcg cgtagagcac ggagcggtac
      481 gggacgcgcg tgccctgcac gatgcggtag tcgtggaacg tgcgcctgta tttgaccggc
      541 gtgccgcccg gctgctgcgc ggtctcgtat tcgagccaca gcaccttccc cgtctgcgag
      601 ctgatgtagt agcgcgtccg cccgcccgct ttctccgtca ggtcgagcac ccacatgtcg
      661 atgttcttct gcttgtcctt gcccacgtag ttgagggtcg cgccgttctc tttgtagcgc
      721 agaaggttct cgatgccgtg ctggcgggcc gagtcgaagt cggaaacgtc ctcctgtcgc
      781 ggggcgaacg aggtgccttt gatgatgccc cacaggcggc cgtcgttgaa gatgagcgag
      841 tactcgagat tggtgcgtct ctgatcgacg cgaatcttgt ccttgtcgct cgtctcgccc
      901 cgcttgaagg agcgctcgta ctcgatctcg accggggtgc cgtccgtagc ggtgcgcgag
      961 atgcgcccgc gctcgacgcc cgtgcgtctc acctgttcga gttgggcgcg gccgccgaaa
     1021 accaacaaca cgccctcggc gatgccttcg gccgtcagcc ccgcgtcact tgtcttctcg
     1081 gccttcttct cgtccttctt cttctcgacc ggggccgccg tttgcgcgtg cgcccccgaa
     1141 gcgacagaag acagggccgc cgcgagcata agtaaatgaa agcgtcgtag cactgttgta
     1201 agcatctccc tattaaaatt ttccctgaga ctcaacaccg ccggcgcccg tgtgggcatc
     1261 gctctgttgg agagcacggc cgagatttga gacgattgta acacaactgc cccgtctggc
     1321 ttcaggcccg gcggcgcgtc agaatctatt gatgccaaag ggttagcggg cgatgcccgg
     1381 cctctgacgt tgacaagctt tcggactaaa atgtagagtg caaagttttc ataacaacgt
     1441 gtgagaggcg cctgctatgt ttggtgcgtg ccgggcgacg cttcagagag cgccgcggcc
     1501 gcagtggccc tttccgaaag gaagtgaaca tcgtgaagag agttggatta ctggtggggc
     1561 gcgagaagtc attcccgcag gccctgatcg aggccatcaa cgagcgcggc gcgggggagg
     1621 tgacggccga gttcatcacc gtcgggggca tccgccacga ctacgaaaaa cagtacgacc
     1681 tgatcgttga ccgcatctcg catgaagtgc ccttctaccg agccttcctc aagcgcgcgg
     1741 cgctcgaagg aacggtcatc gtcaacaacc cgttctggtg gtcggccgac gacaaattct
     1801 tcaactattc gctggccgaa aagttgggcg tcgccgtccc gcgcaccgtc ctgctcccgc
     1861 agaaggacta catcgagggc atcgtcagcg attccctgcg caatctggag ttcccgctcg
     1921 actggcaggg catcacggat tacgtcggct tccccgcctt catcaagccc ttcgacggcg
     1981 gcggctggaa gaacgtctcc aaagttaact cgctcgaaga actctggcgc gtctacgaca
     2041 ccacgggcac gctctgcatg acgctccagc agggcatcga gtacgaccag ttcgtgcgct
     2101 gctactgcat cgggcaggag gacgtgatga tcatgcccta cgacccgtcg aagccctatc
     2161 tttcggggga gcagtacctg cacaatccca cgtatctctc caccgaactc gcgcagcgcg
     2221 tcacagacga cgtgcgcaag ctctgccgcg cgctcggtta cgacatcaac acctgcgagt
     2281 tcgccatcaa ggacggcgtg ccttacgcca tcgacttcat gaacccggcg cccgacgccg
     2341 acctccactc cgtcggcgag ttctatcaca actgggtgac gaaggctgtc accgacctcg
     2401 ttttcaaacg ccttgcggag ccacaacccg ctccgcgcta ccgttgggac gccctgctca
     2461 accccgtgcc tgagcctgcc ggcgcaaggg aggcgctctc ggcaatcgcc acggccgcgg
     2521 aacaggcttc acgcaccatc atcccgcagc ccgtccgtga ttttaccgcc gacgtggccg
     2581 cgggggtcac tgacttggtc gaacaggcgg ccggcgccgt ctcgtcgctc gtcggctcgg
     2641 acgaagtagc cgaggagccg gcggcgaagg ataataaaaa gccgcgctct tccgccgtca
     2701 agggtaagac acccgccaaa aaaagcgcgg ccccgcgcgg ccggaagacg ccttcgacgg
     2761 aagtctagcc ttccccaagc aaaaatcgcc cggcgctgct tacgaaaagt tcaagcagcg
     2821 ccgggcggta acattttgaa gctcggaatt aaatgtgtgt ctaaaaagag ctgtgccagc
     2881 tcgagtgttg cgtttgtggg gggcacaatc ggcgttcgag ctggcactgg cgggcactta
     2941 acttttattc aaggcggtta tccggtggat tgcgctggcg cttggggcgc caacccgtct
     3001 tgaacgagcg gcattataca tcacttcaca aaactgtcaa cgcttttttg tgactaaata
     3061 gacaaataat cgaccgccct gctgatttga caggcccccg ctctaaacct atactttgcc
     3121 actctttcga cgcttttcag cgtgcggcca ggtagctcag ttggtagagc acgcgactga
     3181 aaatcgcggt gtcggcggtt caattccgtc cctggccatc aacaaaatga aaaaacgggc
     3241 agcccgcaag ctgcccgttt ttgctttgtg tgccgaagtg tgccgattag gcccaggctc
     3301 cgcagcatta ttcgacggtg atgttgatct gctgtgacgc ctcggtcttt gccgcgcgat
     3361 cattgacggt gactttcaga gtgtagcgtc ccggaggaaa acctgaaagt ggtatctggg
     3421 cgccgtactg caagcgtgtc tgatccgcga ccccggcggt caagatcggc tgcgggggcg
     3481 aggcgatgag ctgcttgccg ttctgcagca cttgcagtgt gacggccaca tcggcctgcc
     3541 cgttggctga ccgggccgcg ttgtagacga acatctggat gagcatgctg gaagcgcgcg
     3601 caaacctgcg ctccacatcc agcgccgggg cttccccggc cttgcccgta acttcggcca
     3661 gcaggatatt gctcagtgcc agctctccag ccttaaactt gggtatctca agccagagga
     3721 atgagcttcc gacccgcccc gtctgcgcgt cgcgcgccgc gacgcgcacc tgatacaggc
     3781 ccgccggcag agcgttcggc atcgtcgcga caattctaaa aggctgcgtg cttgactcgc
     3841 tgattttgac attctggccc acgcctgtga cggacttgcc tgagtcattc agcaccgcta
     3901 cagcgaagtc aacttcaccc gccgccttac cgcccgcgcc gggcgcgagc tggaagttag
     3961 agaggtctgc gaaagtgatg atcttgtagc tgtcgctgct ctcgttggcg taggtcgtgt
     4021 aaaccgacac acccagttgg cggcgcggga aggccgatga gatggcggcg cgcagatccg
     4081 cctctgaagg tttaagcgcg ggagtcttac tgtctttagc cgtggttttg ttgtccgacg
     4141 cgagcggcgg gggcggtgtg tggtagaagc ccttgcgcga gagcactttc aaatcggagc
     4201 ggcccttgat gctgactttc agactgctga acttgggttt gcccgattcg attacctggg
     4261 gcttccaggc cagcacgtaa taggactctg tttctgtcag cacctgtctg acgccggatt
     4321 ccagatcgtt gcggttgagg atggcgcgcc cgcccgtgtc ggcggcgagc gtgcgcagaa
     4381 tttctctcgt ggcggagtcg gccgacatcg cgatgctgga ggcatcggtc gaagcgtcga
     4441 tgcgctcgcg gccgcccccc tggatactgc caatgccaat atcggagaac acgtcctgac
     4501 tggcggtaac attgctgacg gtgaggccct tggagtccac cgtgtatatg gccacgccgg
     4561 agcgcgcggc cgtatcgatt acgcgttgca atcgttcggt gccttgcgca ctcctcgtgt
     4621 ccaggatgaa gccgtcggag atgaagaaga acagcttgcg tccggggagt gtggccgagc
     4681 cgcgagccac atactcgagt gaaccgagca cggcctcacc gacggcgttg gactggaaga
     4741 gtacggcgcg ggcttcgttc ttgacggcag tttccgccgt catccgacgc atgttcatgt
     4801 ccgtgcctgg gaccctcagg acactggagg atatcccgcc gccaccaccg ccgccaccgc
     4861 cgccgctgcc accgccgcca ccgccgggct gaccggattg ctgcgggggc ggcggcgtcg
     4921 gctgcggctg ctcactcgcg ccccccccct ggcgctcaaa acccatgtcg tccatgtatt
     4981 ctttggcctt gtagtcgatc acgttgcgat gacccttgtc gatggccagg gcttcgtaaa
     5041 cagtcatggc gcggcgtcct tgtgtgggcg ctgagcggga ctggtagtta aggcgggaga
     5101 tgccaagcct gagtgcggtc ttgttgccgg tcaactgttg tagaaaacca atctggccgc
     5161 tgggcgagat gagcagagcc tggtcgtctt cgcccatggc gtccacgaaa ttattgagca
     5221 ggtcgcgcgc acgcttgatt ccctcggggg ccagatgcat gtcatcgaca aagaaaatga
     5281 aggtgcggcc caccgacgtg cccaacgcgg ccgcacgtgg ttgggacggg gtgttgtttg
     5341 gcgttccact cgactcacgc gcggccgcca gttgggcggc ctcacgcgcg cttccggtct
     5401 ccacattttc aaagaaagag atttcctgcg gctggccctc caccgacagc tcgaactgat
     5461 ccttggtcag gcccctgacg gtccggcctt gcttgtccag aacgacaagg tctgtttgaa
     5521 ccagatttgt gctaatccgc agcacttcgt cctgctgcgc cgccggcgga ttctgcgtcg
     5581 cagtctgact aagggcgggc ggcaacaggg aggaaaacaa cagagcacat gacaggagga
     5641 gaccgacgcg agttttaatg taacgatggt gcatgagtta ccttctattt tagacggagg
     5701 ttgaacacag ggcgaataac gcagtgtcgg cgctctcgtt gaaagccggg gagacgtctg
     5761 tgagcgcggc tgaattagaa gaagcgattc ccggcgtccg actaaggtga agcggtctgg
     5821 gccgttcctg cttcggcctg cgttgcgccg agaacctaaa cgaagcagtg ggaatttcgc
     5881 ataaccagtg gggaaaatca agtttttcga cgcggcagat ggttcaactc cgaacctctg
     5941 cgacaaagag tttgtgtgcg tagcggcatg aaccggggat tagaagtata cggggccatt
     6001 ttctgagggt cacaatttag catgattgaa tagctcttcg gcgtgtgccc gtgcctcgga
     6061 gagcacatcg cgctcgacca gcgtctttaa ttcaccatcc ttcatcacca cctcgccgtc
     6121 gataagcgtt gcgcgcacgt ctcccgcttc cgctgcgtag accagtgtcg aaacgacatc
     6181 ggggcgtggc gtcgtgtgcg tgcgctccag atcgacgagg atgaggtcgg cgcgcttccc
     6241 cgcttccaga ctcccgatct cgccggcgag gccgagggcg cgcgcaccgt cggcggtcgc
     6301 catacgcagg acccggcggg ctgggagggc ttccgcgccg cccacgactt tctgaagcag
     6361 cgaggcggta cgcatctccg tgaacatgtc gaggcggttg ttgcacggtg cgccgtcggc
     6421 cccgagcgag acggagacgc cgcgcgcgag catttcgata atgggggcta tccccgatga
     6481 aagcttgagg ttcgacgacg ggcagtgggc aacgtgggtg cccgtctcag ccaacagttt
     6541 ctgctcatcc cggtcaacgt ggacgcagtg cgcgagtacg acatcatccc ccgtcagccc
     6601 gaccgacgag aggtaagaga cgtttcgcat cccggtctcc gccaggacca tctccacctc
     6661 ttcgcggttc tcggacgcgt gcgtgtgaat caacacgccg cgctcgcgcg ccagggctgc
     6721 gacactcctg agcaacgcgt ccgtacagct cacggcgaag cgcggggcga agcagtggcg
     6781 gacgcgcccg ccgccccggc catgccacgc atcgaggagc gcgaccgact ctccgattga
     6841 gtcttcggtc tcctcccgaa gcggcgcggg cacatcgccg cccttgtcca tcatgcactt
     6901 gccgaccgtc gcccgaaaac ccgtctcctc gacgacgcga aagacctcct ccgtgtggcg
     6961 gacggtctcc atcgtgagcg cgcaggtcgt gccgccccga atcatctccg cgatgcctaa
     7021 acgcgccgac gccctgacgg actgcttcga gtgtgcggcc tccatcggcc acacgcgctt
     7081 acgcagccag tcgatgagcg agaggtcgtc ggcggcgccg cggaagagcg tctggcacag
     7141 gtgtatgtgc gtctgcacaa agccggggag gacggcgcag ccgcgtgcgt caatcgtctc
     7201 gtcagcgggg ccgaggtcgt cgccgattgc gacaatgcga ctgccgcgga cgaggaggtc
     7261 ggcggccacg acagagtcgg cggcgtccac cgtgacgagc gtcccgcctt tgatgaggat
     7321 gctgcgttcc aacatgtgac acggcctttg acgcggcctt ccggggcaaa agccgcagga
     7381 ttatgcgccg gggcggcgaa gtctgtaaag aacgccgccc cgcgcacggg cacgaaaaaa
     7441 gtcccggccg ccacagcacg cctccaacgc acgggcgcgg cggcgtgtgt ggcggccggg
     7501 attattctcg gcctctcggc atgcttcgac tacttcttgc cggtcgcagc cgcctcggcc
     7561 tccggctcct cgccgtcggg gccggcgatg ccgagcgcct tgcgaacctc ggtctcgatc
     7621 cggcgcagaa gctcgggatt gtccttgagc gtctgcttgg agttctcgcg gccctgaccc
     7681 aaacgctcgc ccgagtaaga gaaccacgcg cccgacttct cgaccacctt gttggccacg
     7741 ccgagatcga tcacgtcgcc ctcgcgcgag atgccctccc catacatgat gtcgaactca
     7801 cactcacgga agggcggcgc cagcttgttc ttgaccacct tgaccttcgt ccggttgccc
     7861 accgcccggt cgccgtcctt gatggcgccg atgcggcgga tgtcgaggcg aagactggag
     7921 tagaacttca gggcgcgccc gccggtcgtc gtctcggggc tgccgaacat gacgccgatc
     7981 ttctcgcgaa tctgattgat gaagatgagg ctcgtgttcg acgaggagac gacggccgtc
     8041 agtttcctga gcgcctgcga catcaggcgg gcctgaaggc ccggcaggct gtcgcccatc
     8101 tcgccgtcaa gctcagcgcg cgggacgagc gcggccaccg agtcgatgac gatcacgtcc
     8161 acgccgttcg agcggatgag cgcctcggtg atctccagcg cctgctcgcc cgagtcgggc
     8221 tgcgagatca gcaactcgtc gattttgacg cccagcttgc cggcgtactc ggcatccatc
     8281 gcgtgctcgg cgtcgatgta agcggcgaca ccgcccacct tctgcgcctc ggcgacgatg
     8341 tggagcgcga gcgtcgtctt gcccgaagac tccgggccgt aaatctcgat gacccggccg
     8401 cggggcacgc cgccgatgcc gatggcggca tcaaggctta tggaagtggt cgagatcgac
     8461 aacacgtcgg cggcctcgcg gtcgccgaga cgcatgatcg accccttgcc gaatttcttt
     8521 tcgatgttga ggagcgccga ctcgacggcc ttgccgcgct ccgcgttaat tttggaatca
     8581 gccattgtcg ctatcacctt ttctcagtcc cctctcacgc gtttttaaag tcagtgtccg
     8641 gaaaagaccg ggacagtcta ccacaaacgg cgcgagaatg gaacaacgtt tcacacgtgc
     8701 aacagacttt tttctcccgc ccgcacgtga ggaaaggggc cggaggagat tggcccgcgc
     8761 ctcgccgctt gacggtgcaa gcaggagggg ttgagccgtt tgggaaaata tcgcggggca
     8821 ttatagccgc ttcgccggat gccgacaaac agtagcgcga gcggtagtga gcgggtatgc
     8881 ctacgatggc gggccgcatc gcgcaaccac acccgctcac taccgctcgc ggtactgttc
     8941 atccttcggg gacgagtgtg cggacgaaag cgccgacgcg actcacgagg tcgggcgcgc
     9001 ccgcgagccg ttccatctcg gcgacgatgg ccgagccgat gacggccgcg tccgcgtagc
     9061 gccagacttc ggcgacctgc tcgcggctcg aaatgccgaa gccgacggcg accggcaatt
     9121 ccgacacgcg gcgcacgcgg ccgaccaact tttcggcctc cgcgctcatc tccgtgcgcg
     9181 cgcccgtcac gccggcgcgc gagacggcgt agatgaagcc gctcgcctta ccggccacga
     9241 gcgaaaggcg cgcgtcggtc gtcgtcgggg ccacgaggaa gatttgattt acaccgcttg
     9301 cgtccaacac ggcggcgaac tcgcccgcct cctccggcac aaggtcggtg acgagtacgc
     9361 cgtcgatgcc ggcatcacca gccgcacgcc ccagacgttc gacgccatac tgcatgagcg
     9421 ggttgaagta gctgaagagc acgacgggca cgtcggactc gcgccgcgcc tccgacacga
     9481 cggcaagcac gtccgccacg gtcacgcctc cgcgcagggc gcgctccgag gcgcgctgga
     9541 tgacggggcc gtcggcgacg gggtcgctga aggggatgcc gagttcgata atgtcggccc
     9601 cggcgcgcgc gagttcgggg atgagggcgc gggtggtctg gaggtcgggg tcgccggcgg
     9661 tgacgaaggg gatgaacgcg cgccggcctt ctcgtcgcag gcggtcgaaa gtttccgcga
     9721 tgcggctcaa gcggacttct cccctgatgt cacttcgacg gccgccgttt cttccgcgag
     9781 cgcggccacc gtctgcacgt ccttgtcgcc gcgtccagag aggttgacga gaactatctg
     9841 gtcggagcgc atttcgcgcg cgaggcgcag ggcgtgggcg atggcgtgag ccgattcgag
     9901 ggcggggatg atgccttcga gccgtgagag gaggcggaag gcttcgagcg cctctgagtc
     9961 cgaagccgag acgtattcga cgcgggcggt gtcgcgcagg tgcgcgtgct cggggccgat
    10021 tgaggggtag tcgagaccgg cggagacgga gtgtgtcgcg gcgatctgcc cgccgtcgtc
    10081 ctggaggacg tagctcagcg tcccctgaag cactccgggc cgtccgccgc cttccggcat
    10141 gaagcgcgcc gcgtgttcgc ccagaccgcc gccacgccct ccggcctcga cgccgaccat
    10201 gcggacggat gcgtccgaga ggaacgggtg gaagagaccg atggagtttg agccgccgcc
    10261 gacgcaggct acgagcgcgt cgggcaagcg tcccacagtt tcgagaatct gcgcacgcgc
    10321 ctcgcgcccg atgacgctct gaaactcgcg caccatcagc gggtacgggt gcgggccgag
    10381 ggcgctgccg agcaggtagt aggtgtcggc cggcgtagac acccagtcgc ggagcgcctc
    10441 gttgatggcg tccttgagcg tgcgcgagcc ggtcgtgact tcgcgcacct ctgcgccgag
    10501 caggcgcatg cggaagacgt tcaactcctg ccggcgcatg tcctccgcgc ccatgtagac
    10561 gacgcatgag agaccgaaga gggcgcagac cgtggcggtg gccacgccgt gttggcccgc
    10621 gcccgtttcg gcgatgacgc gccgcttgcc catgcggatg gcgagcagcg cctgaccgag
    10681 tgcgttgttg atcttgtggg agccggtgtg ggagaggtct tcgcgtttga ggaagaggcg
    10741 tgcgccgccg gcgtgttcgg tcaggcgggc ggcgtaaaag agaggggtcg gacggccgga
    10801 gtagtgccgg aggagcgatt caaaccgggc gcgaaattct tcgtcatctc tgacttcgag
    10861 gtagcgggcc gtcaactctt cgagcggggc catcaaggtc tcggggacga agcggccgcc
    10921 gtatttgccc cagtagccgc gcgagtccgg caacgcggtg gatgtctctg acatgcggca
    10981 tagcttaaca tccgagactt gatgaaggaa aaaggcaaaa gtgggaaagc caactcgttg
    11041 gcagacatca acgagccgcg gagcggcggc agaatgtagc ccacggcgta agccgtgggg
    11101 acaagccagt ttaaaaaagt gaagccccgg aggggcgaaa gaacctcagc gagcctgatg
    11161 ctcaagttga aagctctttc gcccctccgg ggcttcgttg cacattacgc gaccgttcca
    11221 cgggcttacg cccgtggcta caatctgccg ccgctc
//
LOCUS       DASVES010000131         5476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_793052, whole genome shotgun sequence.
ACCESSION   DASVES010000131 DASVES010000000
VERSION     DASVES010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793052"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="VF240_04745"
     CDS             <1..1487
                     /locus_tag="VF240_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX7174574.1"
                     /translation="SGWVDTKDWLIPITKFLWDDPTVAPRDPAKTTEAELSRQHHFRG
                     VGHLVMRDGFGPDSTWIEFVAGPYLAKHQHLDQLHFAVYHKGYLATESGADYTDTESP
                     HYLNYYRRTIAHNSVLVYDQRERFFWAENLWPAANDGGQRMDSSRYWNTARSREDFQN
                     TRELWALARMEAVGHRPGAYHYARGDATHAYSPVKVELFTRELVYTPSDNVLFVFDRV
                     RATDPTFKKTWLLHGVGEPRIKAAGAGSDAGHGGTSYAGATEFTYEDGSGALRAHTLL
                     PRERELTKRGGKGFEFWTPGDERGGAWGTGQNWPLDPPEGGPLPADPYLRKMWKTFWG
                     EEFEKLLPSNTRAVVPAAWRVEVSPAKPSREDAFLNVMEIGDRGRMPVRRVELADGSN
                     LTGAAVEGGVLALFAAADARVTEGEVTVPDVETTALLVTGLVPNGKYELQLTGGRANW
                     RGGLYNGVPKWQQVATADGSGIIYLPSFKGQRDGRLRLRLLSKT"
     gene            1498..2811
                     /locus_tag="VF240_04750"
     CDS             1498..2811
                     /locus_tag="VF240_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7174575.1"
                     /translation="MMRTAARLYSTALALMLLCGVAAAQRRASDIKEVNQVKGADPGR
                     TVAIVGATLIDGRGGAPIADSMVVVRGERIVAAGPRASVRVPAGAEVFEARGLTLLPG
                     LIDAHFHIDGDDGLPALFLSHGITSLRDPGQWIEAYDAARAAGPPVPRLFLTGPHLDS
                     PPAAYPADSHVVRDAEETRLAVKRFAEQGASAFKVYFRLPVGLIRVLSEEAHRLGLPV
                     TSHLEIVDAADAIRAGVDGVEHVTSFGTALLPARDAEKYRQAVLADNAARREGRYEVW
                     SKADLDGPRARAIISLLAERATYLSPTLAIFERRAGDKDAKDVHVGGFKKMLEFTGRA
                     RRAGVRVVVGSHSAVPKAERGWAYQHELELLVEAGLTPMQAIVAGTSENARFFRVEER
                     LGGVEPGKLADLLLVEGDPLKDIKVMRNVRRVMLGGAWVGATQGK"
     gene            2823..4376
                     /locus_tag="VF240_04755"
     CDS             2823..4376
                     /locus_tag="VF240_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7174576.1"
                     /translation="MKRNILFILHAAALTLALCGAAQAAGKTVHVHLLDGDDARGDGS
                     YAKPYKSWRKALHYAGSGDTVVTKNGDYRKAGQAARWGGIHLVLTMNDKLEPADPRQP
                     VPDPARPETVGIYRYDPARPLTIRAETKHGVRIDSVRLHLARGIVIDGFDINPNPYYT
                     DAAGRKVNRRRNGVHGDSVYEPDDKFNRVKTNDPPGGHTAAWYDRSLWTSYVTVRNCK
                     VHYECPPSGCTPEYDPLQDDDRLYLIKFNQSHHITIEDNELFDGRNAQRKPAVDLPCV
                     DDVVVRRNLIRNSHRGVVSKGGGRRVLIEANVFVDNSGAAFSGGSTDPDLFIDGRVGD
                     PCSFARFESYEMTARDNLVVSTRPGERPVEPISLWAAKDATVVNNTFVGVGERGVLLV
                     RPGNEVDSPARGCGRSVRLTLTEGLTLRHNVFVLSGVVDETMLYQTSGEGVRVAGFVH
                     DQNTFYNGGRDVPVGGLADPNREPGFNKEDALMAGGQGRDYASWMATVKLGKSSPSRG
                     RGVREVSER"
     gene            4458..>5476
                     /locus_tag="VF240_04760"
     CDS             4458..>5476
                     /locus_tag="VF240_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174577.1"
                     /translation="MKPSRGRSLRRSMPFALACVLFLLAHSGEASVQGARGARRVRPQ
                     APNALRLSNQFVSVDWLVTGGRFRTADVVDRVTGKVIRVAEDVFTLVLKDGAEIRSSE
                     MRVVDDLRSGDAPRNPRASRLAERLRGGQAQVTLEDERGRVRATWRAVLRDGSNYVQQ
                     EVTLEALGADLPVSEIRLVDAVLEGARVEGTVSGSPLVAGSVFLGFEHPLSVCKAKPA
                     RATCILPRELPLRAGTNVTYSSVLGVTRPGQLRRDFLAYLERERAHPYRTFLHYNSWY
                     DIGYTNKFDEADSLDRINHFGRELVRKRGVRMNSFLFDDGWDDTSSLWNFHTGFPRGF
                     TPLKEA"
BASE COUNT          890 a         1907 c         1864 g          815 t
ORIGIN      
        1 agtcgggttg ggtggacacg aaagactggc tgatccccat caccaaattc ctgtgggacg
       61 acccgacggt cgcgccgcgc gacccggcga agacgaccga ggcggagctg tcgcggcagc
      121 accacttccg cggcgtcggc cacctcgtca tgcgcgacgg cttcggcccc gactccacct
      181 ggatcgagtt cgtcgccggc ccctacctgg cgaagcacca gcacctcgac cagctccact
      241 tcgccgtcta ccacaagggc tacctcgcca ccgagagcgg cgccgactac accgacaccg
      301 agagcccgca ctacctcaac tactaccgcc gcaccatcgc gcacaactcg gtgctcgtct
      361 acgaccagcg cgagcgtttc ttctgggcgg agaacctctg gccggcggcg aacgacggcg
      421 ggcagcgcat ggactcgtcg cgctactgga acacggcgcg cagccgcgag gacttccaga
      481 acacgcgcga gctgtgggcg ctcgccagga tggaggccgt cggccaccgc cccggcgcgt
      541 accactacgc gcgcggcgac gccacgcacg cctacagccc cgttaaggtc gagctgttca
      601 cgcgcgaact ggtctacacg ccttcggaca acgtcctctt cgtcttcgac cgcgtgcgcg
      661 cgaccgaccc gactttcaag aagacatggc tgctgcacgg cgtcggcgag ccgcgcatca
      721 aggcggccgg ggcggggagt gacgcagggc atggcggcac ctcttatgcc ggcgcaactg
      781 agttcacata cgaggacggc agcggcgcac tgcgcgccca cacactgctc ccgcgcgagc
      841 gagagttgac gaagcgcggc gggaagggct tcgagttctg gacgccgggc gacgagcgcg
      901 gcggcgcgtg gggcacgggg cagaactggc cgctcgaccc gccggagggc gggccgctcc
      961 ccgccgaccc gtacctgcgc aagatgtgga agaccttctg gggtgaggag ttcgagaagc
     1021 tcctgccgtc gaacacgcgg gcggtagtgc ccgccgcgtg gcgcgtcgag gtctcgccgg
     1081 cgaagccttc gcgggaggac gcgttcctca acgtcatgga gatcggcgac cgcggccgga
     1141 tgcctgtgag aagggtggag ctggccgacg ggtcaaacct gacgggcgcg gccgtcgagg
     1201 gcggcgtgct ggccctcttc gcggcggctg acgcgcgcgt cacggagggc gaggtgaccg
     1261 tgccagacgt ggagacgacg gcgctcctag tcacggggct cgtccccaac gggaagtacg
     1321 agttgcagtt gacggggggc cgggccaact ggcgcggcgg cctctataac ggcgtgccga
     1381 agtggcagca ggttgcgacc gccgacggct cgggcattat ctacctgccc tcgttcaagg
     1441 ggcagcggga cggccgcctg cgcctgcgcc tgctctcgaa gacttaggcc tttataaatg
     1501 atgagaacag cagcaagact atattcgacg gcgcttgccc tgatgctcct atgcggcgtc
     1561 gccgcggccc agcgtcgggc gtcggacatc aaagaggtca atcaggttaa gggtgcagac
     1621 ccgggccgca cggtcgccat cgtcggcgcg acgctgattg acgggcgcgg cggggcgccc
     1681 atagccgact ccatggtcgt cgtgcgcggc gaacgcatcg tcgcggccgg gccgcgcgcc
     1741 tccgtgcgcg tgcccgcggg cgccgaggtc ttcgaggcgc gcgggctaac gctgctgccc
     1801 ggcctcatcg acgcgcactt ccacatcgac ggcgacgacg ggctgcccgc gctcttcctc
     1861 tcgcacggca tcacgagctt gcgcgacccc gggcaatgga tcgaggctta cgacgccgcg
     1921 cgcgccgccg gcccgcccgt cccgcgcctc ttcctgacag ggccgcacct cgactcgccg
     1981 cccgcggcct acccggccga ctcccacgtc gtgcgcgacg ccgaggagac acgtctcgcc
     2041 gtcaagcgtt tcgccgaaca gggcgcttcc gctttcaagg tctacttccg actccccgtc
     2101 ggtctcatcc gcgtcctctc ggaggaggcg caccggcttg gactgcccgt cacctcacac
     2161 ttagagatcg tggacgcggc cgacgccatc cgcgcgggcg tagacggcgt tgagcacgtc
     2221 acctccttcg gcacggcctt gctcccggcg cgcgacgcgg agaagtacag gcaggccgtg
     2281 ctcgccgaca acgccgcacg ccgcgagggg cgttacgagg tctggagcaa ggcagacctc
     2341 gacggcccgc gcgcgcgcgc catcatctcg ctgctggccg agcgcgccac gtacctgtcg
     2401 ccgacgctcg ccattttcga gcggcgcgcg ggcgacaagg acgctaagga cgttcacgtc
     2461 ggcggcttca agaagatgct cgaattcacc ggccgcgcgc ggcgcgcggg cgtccgcgtc
     2521 gtcgtcggtt ctcactcggc cgtgccgaag gccgagcgcg gctgggcgta ccagcacgag
     2581 cttgagttgc tggtcgaggc gggactgacc ccgatgcagg ccatcgttgc cggcacctcg
     2641 gagaacgcgc gcttcttccg cgtcgaggag cgcctggggg gcgtcgagcc ggggaaactc
     2701 gccgacctcc tgctcgtcga aggcgacccg ctcaaagaca tcaaggtcat gcgcaacgtc
     2761 aggcgcgtca tgcttggcgg cgcctgggtc ggcgcgacgc agggcaagtg aggatggcag
     2821 agttgaagag aaatatcctt ttcattctac acgcggccgc cttgacgctc gcgctgtgcg
     2881 gggccgcgca ggccgcgggc aagaccgtcc acgtccactt gctcgacggc gacgacgctc
     2941 gcggcgacgg cagctacgca aagccttaca agtcctggcg caaagcgctg cattatgcgg
     3001 ggagcggcga caccgtcgtc accaagaacg gcgactaccg caaggcgggt caggccgcgc
     3061 gctggggcgg catacacctc gtcctgacga tgaacgacaa gctggaaccc gccgaccccc
     3121 gccagccggt gcccgacccc gcgcgccccg agaccgtcgg catctaccgc tacgaccctg
     3181 cgcgcccgct caccattcgc gccgagacta agcacggcgt ccgtattgac tcggtgcgcc
     3241 tccacctcgc ccgcgggatt gtcatcgacg gcttcgacat caacccgaac ccttactaca
     3301 cggacgcggc ggggcggaag gtcaaccgcc gccgcaacgg cgtccacggc gacagcgtct
     3361 acgagcccga cgacaagttc aacagggtga agacgaacga cccgccgggc ggccacaccg
     3421 ccgcgtggta cgaccgctcg ctgtggacga gttacgtgac ggtgcgcaac tgcaaggttc
     3481 actacgagtg cccgccgtcg ggctgcacgc ccgagtacga ccccttgcag gacgacgacc
     3541 ggctctacct catcaagttc aaccagtcgc accacatcac gattgaagac aacgagctct
     3601 tcgacggccg caacgcgcag cgcaagccgg ccgtggacct gccctgcgtg gacgacgtcg
     3661 tcgtgcggcg caacctgata cgcaacagcc accgcggcgt ggtctcgaag ggcggcggga
     3721 gacgtgtgct catcgaggcc aacgtcttcg tggacaactc gggcgcggcc ttctcgggcg
     3781 gctcgaccga ccccgacctc ttcatcgacg ggcgggtggg cgacccctgc tcgttcgcgc
     3841 gcttcgagtc gtacgagatg acggcgcgcg acaacctcgt cgtctcgacc cggccgggcg
     3901 agcgccccgt cgagcctatc tcgctctggg cggcgaagga cgcgacggtc gtcaacaaca
     3961 cgttcgtcgg cgtcggcgag cgcggcgtgc tgctcgtccg ccccggcaac gaggtggaca
     4021 gccccgcgcg cgggtgcggc cgaagcgtgc gcctcactct caccgaaggt ctcacgctcc
     4081 gccacaatgt cttcgtcctc tcgggcgtcg tggacgagac gatgctctac cagacatcgg
     4141 gcgagggcgt gcgggtcgcg ggcttcgtcc acgaccagaa cactttttac aacggcgggc
     4201 gcgacgtgcc cgtcggcggc ctcgccgacc cgaaccgcga gccgggcttc aacaaggaag
     4261 acgcgctcat ggccggcggg cagggccgcg actacgcctc gtggatggcg acggttaagc
     4321 tcgggaagag ttcgccttcg cggggtcggg gcgtgcgcga ggtttccgaa aggtagctgc
     4381 cccgcgtggg tcggccggag tggaacgtcg gaggggagat acttgtgcgc agtgtcgaga
     4441 gagacgggga ttcattcgtg aagccctcac gcggccgaag cctgcgccgc tccatgccct
     4501 tcgcgctggc ctgcgtgttg ttcctgctcg cgcactcggg cgaagcctcc gttcaggggg
     4561 cgcgcggcgc gcggcgcgtg cggccacagg cgccgaacgc cctgagactt tcaaaccagt
     4621 tcgtcagcgt cgattggctc gtcacgggtg gaaggttccg cacagccgac gttgtggaca
     4681 gggtgacggg caaggtcatc cgcgttgccg aagacgtttt tacgctcgtg ctcaaagacg
     4741 gcgccgagat caggtcgtcc gagatgcgcg tcgtggacga tctgcgctca ggggacgcgc
     4801 ctcggaaccc gcgtgcctcg cgcctcgcgg agcggctgcg cggagggcaa gcccaagtca
     4861 cgctcgaaga cgaacgcggg cgcgtgcgcg cgacgtggcg cgccgtgctg cgcgacggct
     4921 cgaactacgt gcagcaggag gtgacgctgg aggctttggg cgcagacctg cccgtctccg
     4981 agattcgtct cgtggacgcg gtgctcgaag gcgcgcgcgt cgaggggacg gtcagtggct
     5041 cgccgttggt ggccgggagc gtcttcctcg ggttcgaaca cccgctctca gtctgcaagg
     5101 ccaagccggc gcgcgcgacc tgtatcttgc cgcgcgagct gccgctgcgc gccgggacga
     5161 atgtcactta ctcgtccgtc ctcggcgtca cgcgcccggg gcagttgcgc cgcgacttcc
     5221 tcgcctacct ggagcgcgag cgcgcgcacc cctaccgcac gttcctccac tacaactcct
     5281 ggtacgacat cgggtacacg aacaagttcg acgaggcaga ctcgctcgac cgtatcaacc
     5341 acttcggccg cgagttggtg cgcaagcgag gcgtgcggat gaactccttc ctcttcgacg
     5401 acggctggga cgatacctcc tcgctctgga acttccacac gggcttcccg cgcgggttca
     5461 cgccgttgaa ggaggc
//
LOCUS       DASVES010000132         8981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1019386, whole genome shotgun sequence.
ACCESSION   DASVES010000132 DASVES010000000
VERSION     DASVES010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019386"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            660..6458
                     /locus_tag="VF240_04765"
     CDS             660..6458
                     /locus_tag="VF240_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7174578.1"
                     /translation="MNNQRPVAHTPTSLLCCVLSYVLLLTALAPFVPRRAAAASRAAK
                     KAAPAARRGQGEGAPRREGEVVVRFRAGVAEDAKGALVSSRGARRKAKLRGESELEKV
                     ALAPGQDASALAEALRLDPAVEFAEPNFLITRSQVTPDDARFGEQWALRNTGQAGGNA
                     GSDVRASTAWQESTGSPTTVVAVIDSGIDFTHPDLTNQQWRNRAEQAGNNADDDGNGY
                     VDDALGWDWVTDSGAVRDEQGHGTAVAGVIAAEGNNSFGTAGVMWRASLMSLRVLDSM
                     GMGDVAAAVEAIDYAAAHGASVVNCSWGTDADSRALRDAVERAGRRGVVVVVSAGNSA
                     RDIDATPYYPASFDLTNQIAVASTDQFDNLAQFSNRGAERVGVAAPGVGVLTTRMGGH
                     HGAVTGTSISAPLVAGIAGLLKTMRPSMPAALVRDAIISGSRRVAVLEGRVASGGVAD
                     AAGAIGALYGNPNGGGGNGNGNGNGNGNGKNGGGNGQGHGRPYVPPGRDDDGSRGRDK
                     KGLIVTPPKEVRGVPAANLPNLDESRKRRTSPVATAPSPAIQSNLACADCDPFGGGGA
                     GGSDPYFGTGRTRPQNDTGDPGVTLGSRNFNWGLPLVGLPGRAGLDLSLTLYYNSLVW
                     TKQNTSVQYNADHGTPAPGFQLGLPRLQNPYYDSDEGANSYVMITPSGGRVQMKQIGA
                     SPPVYESSDGAFTRLTIEGTTPVVRTTDGTQYRFATQAGGERRCTQIKDRNGNFISAT
                     YNATNGHLLTVKDTTERVVTFVYYADGNLQKITQTWGAATHTYATFYYGSVPMSPNFT
                     GVTAVYGLPSGGLQTVLAYVELSNHETYHFDYNAYGQVNQIRHNAPDGYELEHTAYTF
                     DLTGPQTDCPRFTHRRDYARYWNNDAEAVTAYSAAADGSWSRAIPPDGSHHYEYYATS
                     GWQKGLPVRTEVHAEGGLKKWTTIAWAQDDESLPYQKNPRVIETNVHDGTNRRRTTIH
                     YDYAPYTAYGLPHFVAEYAADGVTVVRHTVMEYNLGAAYVDRRIIGLVTWRFVFDGAW
                     NLQAKTYFTYDEQWEGFMVATPGAATQHDSANYGASSVAGRGNLYYEARFDVGDQHNW
                     DKTVRTHWGYNQTGSVTFTSDTSWHMTSFAYGDSFSDGNNARGTYAYPTLVTDPEGYY
                     SIAQYNYDHGGTTLTYTPTSGTGAGTTHVGEAAQYDAHGRIERVTNQTSGAYKRWVYE
                     DNGYYVHTYETIKDTTQANEFHSWSMLDGAGRVRAAASDHPGSAGGFSGVSIVYNNMG
                     RLQQQSKPTEMTGAWAPAGADSSWSYTYQSYDWKGRVTQVLNPDGSTRAFSYGGCGCA
                     GGEVVLTQDEHGRQRRLTNDVLGRLKKVEELDWGGSVYSTSNYTYNTRDQITEVNQQG
                     QIRSFVYDGLGRLLSRTTPEQGATTYTYNLDDTVNTVTDARGASKTFGYNARHLPTSI
                     TYGAPAGVASTPTVTFTYDAAGNRTSMTDGHGAASYHYDSLGRMDWEGRTITEIAPNA
                     TYYLYYGYNVAGQLTSLTNHWGAQVSYDYDRAGQVTSVGGAGYAGVSNYASSLKYRAF
                     GALKEMNFSNGRSLSASYDNRMRPTQWNVAGVLGSNYYYDHFNERTGRVTYAQNLYDA
                     TLDRSYEYDHVGRLVYTHSGAEARAHAYSGQWGVMDGPFSQGYEYDVWGNQTKRYGWG
                     GVLGGAPGSDNQYTTAFTGNRRQGFSYDASGNLTNDLGQTFAYDAEGRQTSAAYGGYS
                     LAQTYDGDRLRVKKVENGGTTYYLRSTVLGGAVVAEMGYVMYYGWAWMRGYVYLGSQL
                     LATQQGGVQFVHEDPVTKSKRVTDMAGTVQSAVELDPFGADTSRSSNASFQPKRFTSY
                     ERDGNQSDEAMHRRFNRFHSRFDQPYVPKIVFVSRMMVLAPRSSALCTMSLVPPSPSP
                     VLPSQSAISVTLSPPAASALCSIQRLLRCQML"
     gene            complement(7277..8248)
                     /locus_tag="VF240_04770"
                     /pseudo
     CDS             complement(7277..8248)
                     /locus_tag="VF240_04770"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            complement(8258..>8981)
                     /locus_tag="VF240_04775"
     CDS             complement(8258..>8981)
                     /locus_tag="VF240_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174579.1"
                     /translation="KYNAADRPDRNTKIPLWQLVRASTAAPVFFPPEVVQWDSEDPSK
                     AFIFEDGGLTPYNNPAFLLMRMATHPAYRLGWQTGERNLLVVSVGTGAAPKVDAEVHG
                     GGKNAFSNLINFPAALMYGAQVDQDINCRVIGRCVHAGAFDRELGRCDEIDSELGDMI
                     PRDEAGNMIPLSQDLGRQFLYARYNAELSKPWLSARGLGDIDPSSVAQLDSVAHMDDL
                     SRIGRRLAQEVSIDHFGPFVGK"
BASE COUNT         1550 a         3074 c         2996 g         1361 t
ORIGIN      
        1 ctcgggcttg cgcccggcgg actgcccgcg ctgctgcgcg actcccgcct cttctcgggc
       61 cgcaagaccg aaatgcacct cttcaacgac gagttccgtc tgcgcctcca cggcgtctga
      121 agggcagtga gcagtaagcg gtaagcagtg agcagtcaag acaagtaagg ccgcccggag
      181 catcatgcac cgggcggcct tcttggttat cgggcggcat tttagtcttc tgaaaatgga
      241 taaagtcgag ttgcgcggtc gaaataattt gcgttgacag tcgccggact cctgcattag
      301 tctccccctt gcttcccggc gcgtttctca cgagagctac cgaacgctcc ccttcgttag
      361 cctcactaac acagtcccga ctggcctgct gatgcactac ccccgcgacg cgcggccgct
      421 gacatgactc actccgacgc tccctgtcgg cagaccacgc cgagcaccgc tcttcgactc
      481 cacgagtcat ggatgagcga gaagcgtcgc cacgtgcgct cagacccggc ccttgaacaa
      541 caccaaagcc tccgacgagc gtcgggcccg aaaggtaagt tgcccggagt ggccgctctc
      601 acgtccttaa cgcgaaactg acggcctcga catcagcgtt cagcgaggaa cctcacgcga
      661 tgaacaacca acgtcccgtc gcccacaccc cgacgagcct gctctgctgc gtcctgtcct
      721 acgtgctgct gctcaccgcc ctcgcgccct tcgtcccgcg tcgggccgcg gcggcttccc
      781 gcgcggcgaa gaaggccgcc cccgccgcgc ggcgggggca gggcgaaggt gcgccgcggc
      841 gtgagggcga ggtcgtcgtg cgcttccgcg cgggagtggc cgaggacgcg aagggcgcgc
      901 tcgtctcgtc gaggggcgcg cggcgcaagg caaagctgcg gggcgaaagc gagctggaga
      961 aggtcgctct ggcgccgggg caggacgcgt cggcgctggc cgaggccctc cgactcgacc
     1021 cggcggtcga gttcgccgag cctaacttcc tcatcacgcg ctcgcaggtc acccccgacg
     1081 acgcgcgctt cggcgagcag tgggcgctca gaaacacggg gcaggcgggc ggcaatgccg
     1141 gctccgacgt gcgcgcctcc acggcctggc aggaaagcac cggctcgccc acgacggtcg
     1201 tcgcggtcat cgacagcggc atcgacttca ctcaccccga cctcacgaat cagcagtggc
     1261 gcaaccgggc cgagcaggcc ggcaacaacg cggacgacga cggcaacggc tacgtcgatg
     1321 acgcgctcgg ctgggactgg gtgacggatt cgggcgcggt gcgcgacgag caggggcacg
     1381 gcacggccgt cgcgggcgtc atcgccgccg agggaaacaa ctccttcggc acggcgggcg
     1441 tgatgtggcg cgcgagcttg atgagtctgc gggtgctcga ctcgatgggg atgggcgacg
     1501 tggccgcggc cgtcgaggcg atagattacg ccgccgcgca cggcgcctcg gtcgtcaact
     1561 gctcgtgggg cacggacgcc gactcacggg cgctgcgcga cgccgtcgag cgtgccggcc
     1621 gtcgcggcgt ggtggtcgtc gtctcggccg gcaactccgc ccgagacatc gacgcgaccc
     1681 cctactaccc ggcctccttc gacctgacga accagatcgc cgtggcctcc accgaccagt
     1741 tcgacaatct ggcgcagttc tccaaccggg gcgcggagcg cgtcggcgtc gccgcgccgg
     1801 gcgtgggcgt gctcacgacc cggatgggcg gccaccacgg ggccgtgacc ggcacgtcca
     1861 tctcggcgcc tctggtcgcg ggcatcgcgg gtctgttgaa gacaatgcgt cccagcatgc
     1921 cggccgcgct cgtgcgcgac gcgatcatca gcggctcccg gcgtgtggcg gtactcgagg
     1981 gcagggtcgc gtcgggcggg gtggccgacg ccgccggagc aatcggcgca ctctacggca
     2041 accccaacgg cgggggcggg aatggaaacg gcaacgggaa tggcaatggc aacggcaaga
     2101 acggcggcgg caacgggcag ggccacggtc ggccatatgt cccgccgggc cgcgacgacg
     2161 acggctcccg tggccgggac aagaagggac tgatcgtcac cccgccgaaa gaggtgcggg
     2221 gcgtgccggc agccaacctg cccaacctcg acgagtcgcg caagcgccgc acgtctcccg
     2281 tcgccacggc cccttcgccg gccatccagt cgaacctggc ctgcgccgac tgcgacccct
     2341 ttggcggcgg cggggccggc ggcagcgacc cctacttcgg caccggccgc acccggcccc
     2401 agaacgacac aggcgacccc ggcgtcaccc tcggctcgcg caacttcaac tgggggctgc
     2461 cgctcgtcgg gctgccgggc cgcgcggggc tagacctctc gctcacgctc tactacaact
     2521 cgctggtgtg gactaagcag aacacttccg tccagtacaa cgccgaccac ggcacgcccg
     2581 cgcccggctt ccaactgggc ctgccgcgtc tccagaaccc ctactacgac tccgacgagg
     2641 gggccaactc ctacgtcatg atcaccccgt cgggcgggcg cgtccagatg aagcagatcg
     2701 gcgcgtcccc cccggtctac gagtcgagcg acggcgcctt cacgcggctc accatcgagg
     2761 gcacgacgcc cgtcgtccgc accacggacg ggacgcagta ccgcttcgcg acgcaggccg
     2821 ggggcgagcg gcggtgcacg cagatcaagg accgcaacgg caacttcatc tcggcgacct
     2881 acaacgcgac gaacggccac ctgctcaccg tcaaggacac cacggagcgt gtcgtcacct
     2941 tcgtctacta cgccgacggc aacctccaga agatcacgca gacgtggggc gcggcgacgc
     3001 acacctacgc caccttctac tacggctcag ttccgatgtc gccgaacttc accggggtga
     3061 cggccgtcta cggcctaccg agcggcgggc tccagaccgt gctcgcttac gtggagttgt
     3121 ccaaccacga gacatatcac ttcgactaca acgcttacgg gcaggtcaac cagatcaggc
     3181 acaatgcgcc ggacggttat gagcttgagc acaccgccta caccttcgac ctgacggggc
     3241 cgcagacgga ctgcccccgt ttcacccacc ggcgcgacta cgcgcgatac tggaacaacg
     3301 acgcggaggc cgtcaccgct tacagcgcgg ccgccgacgg ctcttggagt cgcgccatcc
     3361 cgcccgacgg gtctcaccat tacgagtact acgcgacctc gggctggcag aagggcttgc
     3421 ccgtgcgaac ggaggttcac gccgagggcg gtctgaagaa gtggacgacc atcgcctggg
     3481 cgcaggacga cgagagcctg ccctatcaga agaacccgcg ggtgatcgag acgaacgtcc
     3541 acgacgggac gaaccgccgg cgcacgacca tccattatga ctacgccccc tacaccgcct
     3601 acgggctgcc acatttcgtg gccgagtacg ccgccgacgg cgtgacggtc gtgcgccaca
     3661 ccgtcatgga atacaacctc ggcgccgcct acgtagaccg ccgcatcatc gggctggtca
     3721 cctggcgctt cgtcttcgac ggggcgtgga atcttcaagc gaagacttac ttcacctatg
     3781 acgaacagtg ggaaggcttc atggtggcca cgcccggcgc ggccacgcag cacgactccg
     3841 ccaactacgg cgcaagctcc gtggcggggc gcgggaacct ttactacgag gcgcgcttcg
     3901 acgtcgggga ccagcacaac tgggacaaga cggtacggac gcactggggc tacaatcaga
     3961 cggggtcggt caccttcaca tcagacacct cctggcacat gacctcgttc gcctacgggg
     4021 attctttctc cgacggcaac aacgcgcggg gcacctacgc ctacccgacg ctggtcactg
     4081 accccgaagg ctattactcg attgcgcaat acaactacga ccacgggggc acgacgctca
     4141 cctacactcc gacgagcggc acgggggcgg gcacaacgca tgtcggcgag gcggcgcagt
     4201 acgacgccca cgggcgcatc gagcgcgtca ccaaccagac gagcggcgcc tacaagcgtt
     4261 gggtctacga ggacaacggc tactacgtcc acacctacga gacgatcaag gacacgacgc
     4321 aggccaacga gttccactcg tggagcatgc tggacggcgc ggggcgagtg cgcgccgcgg
     4381 cctccgacca ccccggcagc gccggcggct tcagcggggt atcgattgtc tataacaaca
     4441 tggggcggct tcagcagcag tcgaagccca cggagatgac cggcgcgtgg gcgccggcgg
     4501 gcgcggactc atcgtggtcc tacacgtatc agagctacga ctggaagggc cgcgtcactc
     4561 aggtccttaa cccggacggc tcgacccggg ccttctccta cggcgggtgc gggtgcgcgg
     4621 gcggcgaggt ggtcttgact caggacgagc acgggcgcca gcgccgcctg acgaatgacg
     4681 tgctcggcag gctcaagaag gtcgaggagc tggactgggg cggctccgtc tactcgacga
     4741 gtaactacac ctacaacacg cgcgaccaga tcaccgaggt caaccagcag ggtcagatca
     4801 ggagcttcgt ctacgacggg ctggggcgct tgctgtcgcg caccacgccc gagcaggggg
     4861 cgaccacata cacttacaac ctcgacgaca ccgtcaacac ggtgacggac gcccgcggcg
     4921 cgagcaagac cttcggctac aacgcccgcc acctcccgac ctcgatcacc tacggggcgc
     4981 cggcgggcgt cgcctcgact cccacggtga cgttcaccta cgacgcggcc ggcaaccgca
     5041 cgtcgatgac ggacggccac ggcgcggcca gctaccacta cgactctctg gggcggatgg
     5101 actgggaggg gcgcaccatc acggagatcg cgccgaacgc cacctactat ctctactacg
     5161 gctacaacgt ggccgggcag ctcacgtcgc tcaccaacca ctggggcgcg caggtctcct
     5221 acgattacga ccgggccggg caggtgacga gcgtcggcgg ggcgggctac gccggggtgt
     5281 cgaattacgc cagctcgctc aagtaccgcg ccttcggggc gctcaaggag atgaacttct
     5341 ccaacggccg ctcgctgtcg gcctcctacg acaaccggat gcggcccacc cagtggaacg
     5401 tcgcgggcgt gctcggctcc aactactatt acgaccactt caacgagcgc accggaaggg
     5461 tgacctacgc gcaaaacctc tacgacgcga cgctcgaccg ctcctacgag tacgaccacg
     5521 tggggcggct ggtgtacacg cactcgggag cggaggcgcg ggcccacgcc tactccgggc
     5581 agtggggggt gatggacggc cccttctcgc agggctacga gtacgacgtg tggggcaacc
     5641 agaccaagcg ttacgggtgg ggcggggtgc tgggcggcgc gccgggttct gacaaccagt
     5701 acacgacggc cttcaccggc aaccgccggc agggcttcag ctacgacgct tcgggcaacc
     5761 tcaccaacga cctcggccag accttcgcct acgacgccga aggcagacag acctcggccg
     5821 cctacggggg ctactcgctc gcgcagactt acgacggcga ccggctgcgg gtgaagaagg
     5881 tggagaacgg cgggacaact tactacctgc gctcgacggt gctgggcggg gcggtcgtcg
     5941 ccgagatggg ttacgtcatg tactacggct gggcgtggat gcgcggctac gtctacctcg
     6001 gctcacagct cttagccacg cagcagggcg gggtgcagtt cgtccacgaa gacccggtga
     6061 cgaagtcgaa gcgggtgacg gacatggcgg gcaccgtcca gagcgcggtg gaactcgacc
     6121 cgttcggggc cgacacctcg cgcagctcca acgcctcgtt ccagccgaag aggtttacct
     6181 cctacgagcg cgacggcaac cagagtgacg aggccatgca ccgccgcttc aaccgcttcc
     6241 actcgcgctt cgaccagccc tatgttccga agattgtgtt cgtgtcgagg atgatggtct
     6301 tggccccgcg ctcgtcggcg ctctgcacca tgtccctcgt gccgcccagc ccgtcgcccg
     6361 tcttgccgtc ccagagcgcg atcagcgtca cgttgtcgcc gccggcggcc agcgcgttgt
     6421 gcagcatcca gagattgttg cgctgccaga tgttgtagtc gggcttctcc tgaagccagc
     6481 gcggcaactc cttcgactcg ccgagcacgc gcgtgggcag actccgttgg agccgttgga
     6541 agcgctcgac ccactgcggc ccggcggact gaaccgactc cctgacgtac tgctcgcgcg
     6601 ggagcgcgag gaagaggcgg gtcgggatgt ctaacttctc gcagacctcg tggaagatga
     6661 tgtcgccgcc gctcgcgccg ccgctgaggc cgtaggcgac gccgcccgcc aactcctgct
     6721 cgcgcagcac cgtctccatg attttttgtc gcgccacctc ttcctggtcc ggcgggaagc
     6781 gcggctgcgc gcggtctggc gtgtcgatca tgtggccggt gaaaaggagc acccgcgtcg
     6841 ggccggcggg cgcgggtggt gacgcgctgt cgccgccggc gggcgcgggg gcgagcaccg
     6901 ccagagctgc cgcgactttc tcctgaagca ggccgagttc ctgataaagc ccgagctggc
     6961 cgcgtatggc ggccacgacc agggccggcg cgccggcgag cgcccgctgg taggcgttcg
     7021 tgaccctgct cggtttccgc gaggtgagga agcacaggtc ggcctcgctg atctgcgccc
     7081 agatgtcggt cgtctgctcc ctcttgagac gttcggccgc ggccttcaga gagagttcga
     7141 ccgccgccga gagcttttcg gcccgcttct tccgcgccga cagctcgcgc tcggactcgt
     7201 cgtcctcttc aaacctctcc gcccacacct cgggcaacga ttttgccaac tcggtctgga
     7261 cggtcaacat cgccagcgca ttgagtcccg aatagaaatg attgaggtct tcatcgaagg
     7321 ccccttcgta gccttcaagc gactcgtcca gataggccga ctgcaacgcc agccgctgcc
     7381 gctccggcaa cgacgacgcc tgttcccaat cgttcgtcca gagggccttg gcgttacggc
     7441 ccagcagcga atgcgcctcg gcccggtcgt tactgtcgag acctctgcgg ctgaggacgc
     7501 gcttcaacgc ctgatcggag cgcaccagat cgccgaggcg ttgatagatg gtgccgagca
     7561 gcgtgttggc ctccagatcg gacaggtcga gctggcggac ggcctcccac gtcgcgcgcg
     7621 cgccctcgaa cgccttcagg ctgtactgcg cgcgcccgac gaggcgcagc ccctcgctct
     7681 cccactcgaa ccctcgtgct tcgactccga gcagttcgag gtcgccgagt tgccggtcgg
     7741 ccgcggcgcg ctcgacctcc tcgcggaagt ccggcgggac ggcgaggaag tgcgagcggt
     7801 cttgcgcctc aaggctcggc agagacctga agacggggct gtcctgctcc tcggagttct
     7861 tagtctgccg cagcgccgcg acgagcagcg ggaggctggc ggcggggttc gcccggtcgt
     7921 aggtgaaata gcggtcggtc tgcaagtcga aggggaagcg gtcggtgtcg ctgcgcagca
     7981 ggaaggtgcg cttgtcgcgc agggcgtggc ggatgccgag ttcgtagaag acgttggcgt
     8041 tgtgaatcga gaggtcggcg acgacgaggt cggcggtcag caggcgctgg aacatgtcga
     8101 tgcggatgtt gcccgcccgc aggatgtcga tggtggtgcc gccggtgaag ccggcctcgc
     8161 gcagcgccgg ggcgatcaac tcccgctcga cggcgtcgaa gtcgatgtcg tttttgacgc
     8221 cgaaggggcg gacgatgaag gcgcgcatga gttgtcctca ctttccgacg aacgggccga
     8281 agtggtcgat gctgacctcc tgcgcgagac gccggccgat gcggctgagg tcgtccatgt
     8341 gagcgaccga gtcgagctgc gcgacgctcg aagggtcgat gtcgccgagg ccgcgcgccg
     8401 agagccacgg cttggacagc tccgcgttgt agcgcgcgta gaggaactgc cgccccaaat
     8461 cttgtgagag cggaatcatg ttccccgcct cgtcgcgcgg gatcatgtcg cccagctcgc
     8521 tgtcgatctc gtcgcagcgg cccagctcgc ggtcgaacgc gccggcgtgg acgcaacggc
     8581 cgatgacacg gcagttgatg tcctgatcga cctgcgcgcc gtacatcagc gcggcgggga
     8641 agttgatgag gttggagaag gcgttcttgc ccccgccgtg aacttcggcg tccaccttcg
     8701 gcgcggcgcc ggtgccgacg gagacgacga gcaggttccg ctcgccggtc tgccagccca
     8761 gacggtaggc gggatgcgtc gccatgcgca tcagcaggaa ggccgggttg ttgtagggcg
     8821 tcaagccccc gtcctcgaag atgaacgcct tcgaggggtc ttcggagtcc cactgcacga
     8881 cttcgggcgg gaagaagacg ggcgcggcgg tcgaggcgcg cacgagctgc cagagcggaa
     8941 ttttcgtatt gcggtcgggg cggtcggcgg cgttgtactt g
//
LOCUS       DASVES010000133        11725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_726301, whole genome shotgun sequence.
ACCESSION   DASVES010000133 DASVES010000000
VERSION     DASVES010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726301"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..645
                     /locus_tag="VF240_04780"
     CDS             133..645
                     /locus_tag="VF240_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174580.1"
                     /translation="MQNSFRFDVKTRWLLLATSALVIYTVVPIRRGALYDLNAVSTWL
                     QFLLFVLTFPLGSFFVLFVHAFAGGFGEFGIVEQFQMWIVAVALGFLQWFFLIPAMLR
                     HKSSEVTALHLSSGGDDPAVASAQTGGALPEVDTNVATNASPIPQFDERGRTPFERIL
                     NSDDESNKTH"
     gene            932..1843
                     /locus_tag="VF240_04785"
     CDS             932..1843
                     /locus_tag="VF240_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174581.1"
                     /translation="MKKYPLTLSAACALLLFAAQAVAQTLPPPPNVLVFVREDIKPGK
                     MPAHNEEAANFVRVQTKANTVSPKELRDYRLAMVPIAGNENEVTYVWGYNSFEEMENK
                     RREADRLANGPMKADFDALVDRDLHAAQHDIITTFRPDLSHGLGRVDIAQARYTVMTT
                     LRIKPGHEEEYWNAVKKHVYPARDKTAMKNASWAVFAVRAGMPGSSYIILRPVKSLAE
                     FDAASPATVRGVMSGDEKEEMDKVSDRSILLTNTNYYAFSPRLSLVTPEWAARDTAST
                     PFWNPKPQAADPTTSAGSRPAGSRRRP"
     gene            complement(2021..2839)
                     /locus_tag="VF240_04790"
     CDS             complement(2021..2839)
                     /locus_tag="VF240_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX7174582.1"
                     /translation="MSAAGDPPPNEKITRALTRVLRDGALAVLVTIVEAPRGVGAKIL
                     AEESGALVGTTGDASLDAALRAQARVFLDSRAEARTLPVEEFAPALEEWRGARVLFER
                     LEPEPRVVVCGAGHVGAALARLAHAVGYRVTLIDDREDFVARSRFPEEGIELVAASDW
                     TRPVREAIGTGRGVYAAVVTRGHSEDEECMRAVLAARPRYVGMIGSRRRTNIVLERLR
                     EAGLPEELLREVRAPVGLDIGAVSPEEVALSILAEIVAHRRGGTGESLSAWRRK"
     gene            complement(2845..3159)
                     /locus_tag="VF240_04795"
     CDS             complement(2845..3159)
                     /locus_tag="VF240_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEX7174583.1"
                     /translation="MSTSFVEEYKAKHQHPLNKLAHSIGIPMIVVSLPLFFWNWRWAL
                     ALFVVGWILQFVGHFIEGNSPAFFRNPIYLLVGPLWLARRALAAVGLARPARKTLDKR
                     AH"
     gene            complement(3244..3579)
                     /locus_tag="VF240_04800"
     CDS             complement(3244..3579)
                     /locus_tag="VF240_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX7174584.1"
                     /translation="MLSQLELYRLVEEANARGERVVVATVAHTRGSTPQRRGAKMLFF
                     EDGRVAGTVGGGCIEAEVWAEARETMRTGAAALHKFSLTAEAASEEGMVCGGTMEIFI
                     DAWNGKSDE"
     gene            complement(3668..4510)
                     /locus_tag="VF240_04805"
     CDS             complement(3668..4510)
                     /locus_tag="VF240_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174585.1"
                     /translation="MKFKTSLTSGAVALILLLTATMFVAEALPQQRGRRPSRRATAPA
                     RARGDVLPAPTPLPAEPSVISTAEDVAAQDDDTPPRRRNSRTTRRAAAQEADEQQRRE
                     EEMRNSVNRLTQTVTRLSDELTAMKGEQRVLVDLERLTRAEQRAESLRSQLRDVTDKE
                     FVLQDRLAQITAELDPGAIERRSLLIGSLNPANVREQIRLSLEREKERVSRQLELLNT
                     SRVRLESAVAAAEQDAERIKARIDAIEQQQTDANAAGNQTTITQPAPTPTPTSNPPAE
                     PPQE"
     gene            complement(4566..5420)
                     /gene="nadC"
                     /locus_tag="VF240_04810"
     CDS             complement(4566..5420)
                     /gene="nadC"
                     /locus_tag="VF240_04810"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7174586.1"
                     /translation="MKWLDEGALFSQISAFLSEDLGRGDITTQATVMRQQRARGKFLA
                     KEPMVVAGLEAAEAVFSTLDSGQQLEAFASDGDEVEAGKTIARTAGFADVLLGAERVA
                     LNLLQRLSGIATLTREFVKAVEGTEAQIVDTRKTAPGLRMLEKYAVLAGGGRNHRFGL
                     DDGVLIKDNHIALAGGVATAVERARDAVGHLHKVEVEVASERDLREAIRAGADILLLD
                     NQTPEETTRLVLIARELEPAILLESSGGITLANVRAYAEAGVDLISVGALTHSARAVD
                     ISFKIQPG"
     gene            complement(5417..8128)
                     /locus_tag="VF240_04815"
     CDS             complement(5417..8128)
                     /locus_tag="VF240_04815"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX7174587.1"
                     /translation="MEIPKTYDPRQAEEQHYARWERAGCFAPEANRDESAPVYSIVIP
                     PPNVTGSLHMGHALQHTIMDVLARYHRMRGKRTLWVIGTDHAGISTQIVVERELKKEG
                     LSRHDLGREKFIERVWEWKQRSGGRITEQMRREGASVDWSRERFTMDDDLSRAVREFF
                     VRCHDEGLIYRGERLINWCPKDQTALSDLEVDKETQKGQLWHLQYPVKGSDERRVTVA
                     TTRPETMLGDTAVAVNPDDERYQDLIGATLMLPLTGREIPIVADAAVDPAFGTGAVKV
                     TPAHDPTDYEIGERHNLPQIVVIDKHARMTEAAGAEFAGLDRYEAREKVVQKFEELGL
                     LERVADYEGAVSRCDRCRTVIEPLISLQWFCKQRELAEKGLELIRERHEPHFVPEVPW
                     EKVYTDWLEGLHDWTLSRQLWWGHRIPAWYDEEGNVYVARSEEDARVAAGADKLTQDP
                     DVLDTWFSSALWPISTLGWPEETSADLKTFYPTDVLVTAREIIFLWVSRMVMTGTKFI
                     GPLPFRDVVITGLVLDKHGKKMSKSKPSDAVDPMDVFEKYGVDATRITLASSPASTGT
                     DSRWRDEQVESYRNFANKIWNAARFCLMNSEGAQVGKGFEGDPSGWALHDRWIMSELN
                     RTAREVSRAVEGYQFHEAVYSLYHFFWDDFCDWYIELTKQDVTASEPTPARDAARSRV
                     VTVLETALRLLHPFMPFITEELWQRLPGVGADSLHPAYRGMEPTVMLTEYPRADESLI
                     DERAESEMRAVIELVSRVRNIRTELNVKPSEEVRLIISAGGDGLGRVFDASLPQIKRL
                     VRASEVSFKESMADAPRASARAALAGGAELAVPFEGLIDFGQETARLGKEIEKLSKEL
                     ERLEAQLANPQFVERAPAEKVEELRQRSEDIRQRIVALEQMLEALKG"
     gene            complement(8231..9721)
                     /locus_tag="VF240_04820"
     CDS             complement(8231..9721)
                     /locus_tag="VF240_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7174588.1"
                     /translation="MQTRRPKLLLADDSLTIQKVVTLTFTDEGMEVVSAGTGEEAIRI
                     MEESGPPDVLLADVHMPGMSGYEVCERVKQDALWHHVPVVLLKGAFEPFNEAEARRVG
                     ADEVLSKPFQSIKDLIGKVGSLLGGKDAAQASRDDERRVDDERRHEHATDAYAAGVRE
                     ATTSAVVAQDADAKRAPQAGAFATDFAMDDEMIEATPAESFGRRDEAAHTELADVGAA
                     FAGDAVAEARPVERREESAYAAAAGREVAAESQTGSSPFASRASSVATADESLLDLGV
                     ADEPSSAPAEMDDFVLDLDFDEPPPASAGSAAAPAAYGGGQFVPQVDTAGAFAEAAHG
                     SGETYGDDAPAAAHAGYDFSAESAPREDMYATQDVSAMRDFVEPQVVASDEPVRMDED
                     LSVEGDVARPPVAAAPSSWDFSDAAVAHEEESAPGEVRDESLESVDTAAAASTLTPGQ
                     LSPEMIDAIARRVVELMSERAVQEIAWEVVPALAERIIRQRLDEEK"
     gene            complement(10081..10302)
                     /locus_tag="VF240_04825"
     CDS             complement(10081..10302)
                     /locus_tag="VF240_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174589.1"
                     /translation="MLLFRSEEHVERWCAQWRMPRGESFPVERCWRLALAWFGADRRE
                     PLWRRRTAEECEAVFASLDFTSPFWRMTR"
     gene            complement(10309..10776)
                     /gene="merB"
                     /locus_tag="VF240_04830"
     CDS             complement(10309..10776)
                     /gene="merB"
                     /locus_tag="VF240_04830"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HEX7174590.1"
                     /translation="MTETDEQAAFDAEVRRYVYEHAMTEGVPPQSAVAAAALSCSTDE
                     VRAAFRRLADARVLVLQEGGGEILMAAPFSAVPTPFVVRSGGRAYFAPCAWDALGVPV
                     VLGRDAEVEASCGCCGTRMSLRVEGGVLVESEPGSVVHFALPAARWWENVVFT"
     gene            complement(10822..>11725)
                     /locus_tag="VF240_04835"
     CDS             complement(10822..>11725)
                     /locus_tag="VF240_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HEX7174591.1"
                     /translation="KAIKDSDARKVIAATPGFDLRTGRVRVKDVSLAGATPVVVNAEV
                     EIAFGFAKTEAGGAGARWRAVEFRTGDRAWEEFDYLAGPLGVERVEAARLELEALAAE
                     FETEQRERKAAEEAAGKGEKSKDNKLEKEKSEEGKTKDEKAEEEKKLEVRRGPLVMKE
                     FAPMYKSARAVVVVDASFRLEKDTRGKWRVVGFSVGEASFGDFDALIAGVNARKAVRA
                     RADLEAVRAALEAYRRERGFYVVAEDETVLIDHLSPRYLKTVIRIDPWHRPYRYAGTT
                     ERFTLGSDGPDGLPATADDVTISG"
BASE COUNT         1869 a         4007 c         3800 g         2049 t
ORIGIN      
        1 ctcgcggtac tgttcgcgtc tcgtccggcc gtccgatgga acaatgccgc cggccccggt
       61 gtctaaccgg cgaaggactc cgaatcatga cgggcgatgc cgggccgggc agcccgcgtt
      121 ggaggggcgc acatgcagaa ctctttccga ttcgacgtga aaacccgttg gctcctgctc
      181 gccacgagcg cgctggtcat ctacactgtc gtgcccatcc gccgcggcgc gctgtacgac
      241 ctcaacgccg tcagcacatg gctccagttc cttttgttcg ttctcacttt ccctctcggc
      301 tcattcttcg tgctgttcgt ccacgcgttc gccggcggct tcggcgaatt cggcatcgtc
      361 gagcagttcc aaatgtggat cgtcgccgtc gcgctcggct tcctccagtg gttctttctc
      421 atcccggcca tgttgaggca taaaagctct gaggtgacgg cccttcatct ttcctccggc
      481 ggggacgacc cggccgtcgc ctccgcgcag accggcggcg ccctgcctga agttgacacg
      541 aacgtagcga cgaacgcctc acccatcccg cagttcgacg agcgcgggcg cacgccgttc
      601 gagagaatcc tcaacagcga cgacgagagc aacaagacgc attgaagcgc gcctccgctc
      661 ccgcccgtcg gcttccggcc attgacttgc caactcattt gaaatatcgt cccgccacgc
      721 ccagggtcta aagtcttttc cggcgggagc cgattcgcgt cccgcacccc gttttcaacc
      781 actgacggcc gccgccgcgc ggacagccca cgccgtgggc ctcgcctccc gcacacgaac
      841 acgctccacc atccagcgat ccatttgtcg ggtgacgggg cgccgaagtc caaccttcct
      901 taatcaatgc ccatggaggt gggcaccacc catgaaaaaa tatcccctga cactttctgc
      961 cgcgtgcgcg ctacttctgt tcgccgctca agcggtcgcc cagacgctgc ccccgccccc
     1021 caacgtcctc gtgtttgtgc gcgaggacat caagcccggg aagatgcccg cccataacga
     1081 agaggcggcg aatttcgtgc gggtgcagac aaaggccaac acggtgtcgc ccaaagagct
     1141 gcgcgattac cggctcgcca tggttcccat cgccggcaac gagaatgagg tcacctacgt
     1201 ctggggttac aactcgttcg aggagatgga gaacaagcgg cgggaggccg accggctcgc
     1261 gaacggcccg atgaaggccg acttcgacgc cctcgtcgac cgcgacctgc acgccgcgca
     1321 gcacgacatc atcaccacct tccgccccga cctcagccac ggtctgggca gggttgatat
     1381 agcgcaggcg cgctacacgg tgatgacgac gctgcgcatc aagcccggcc acgaggagga
     1441 gtactggaac gccgtcaaga agcacgtcta tccggcgcgc gacaagacgg cgatgaagaa
     1501 cgcttcgtgg gccgtgttcg cggtacgcgc cgggatgccc ggcagcagtt acatcatcct
     1561 gcggccggtg aagtcactgg ccgagtttga cgccgccagc cccgcaaccg tgcgcggcgt
     1621 gatgagcggc gacgagaagg aggagatgga caaggtctcg gatcgctcga ttctcctcac
     1681 caacactaac tactacgcgt tcagcccgcg gctcagcctc gtcacgccgg agtgggcggc
     1741 gcgcgacacg gcctccacgc cgttctggaa cccgaagccg caggcggccg atccgacaac
     1801 gtccgccggt tccagacctg cgggcagcag gcgccggccc tgacgccaac tgatccttcg
     1861 gtcgcgtaag ccgcgccctc aaagggccgc aaccgcagcc ccgaccgggc ggttgcggcc
     1921 ctttctcttt caatgtcatt gtctggctga cgaagattac gggcgggcgg gcttgatttc
     1981 aggttcggac gtgagcgtgc gccgcggctc ggcgtgaaat ttacttccgc cgccatgccg
     2041 agagcgattc gccagtaccg ccgcggcggt gcgcgacgat ctcggcgagg atcgagagcg
     2101 ccacctcctc gggcgagacc gcgccgatgt cgaggccgac gggcgcgcgc acctcacgca
     2161 acaactcttc gggcagtccc gcctcgcgca gacgttcgag cacgatgttc gtccgccgcc
     2221 ggctgccgat catgccgacg taacggggcc gcgccgcgag caccgccctc atgcattcct
     2281 cgtcctcgct gtgcccgcgc gtgacgaccg ccgcgtacac gccccgcccc gtgccaatcg
     2341 cctctctgac cggccgcgtc cagtccgacg cggctaccag ctcgatgccc tcttcgggaa
     2401 agcgcgagcg cgcgacgaag tcctcgcggt cgtcgatgag cgtcacgcgg tagccgacgg
     2461 cgtgcgcgag gcgcgccagc gccgccccga cgtgacccgc cccgcacacc acgacgcgcg
     2521 gctccggctc caaccgttcg aagagcacgc gcgccccgcg ccactcttcg agcgccggcg
     2581 cgaactcctc taccgggagg gtgcgcgcct ccgcgcgcga gtcgaggaag acgcgcgctt
     2641 gcgctcggag cgccgcgtcg agcgacgcat cgccggtcgt ccccacgagc gcgcccgact
     2701 cctcggcgag tattttcgcg ccgacgccgc ggggcgcttc cacgattgtg acgaggacgg
     2761 ccagcgcgcc gtcgcggagg acgcgcgtga gcgcccgcgt gattttttcg ttgggtggtg
     2821 ggtcgccggc ggcgctcatg ccggtcagtg cgcgcgcttg tcgagcgttt tgcgggcggg
     2881 gcgagcgagg ccgaccgccg ccagcgcgcg gcgcgcgagc cacagtgggc cgacgaggag
     2941 atagatgggg ttgcggaaga aggcggggct gttcccctcg atgaagtgac cgacgaactg
     3001 caaaatccag ccaacgacga agagcgcgag cgcccaccgc cagttccaga agaagagcgg
     3061 caggctgacg acgatcatcg gaatgccgat ggagtgcgcc agcttgttca aggggtgctg
     3121 gtgcttggcc ttgtactctt caacgaagga cgtggacatc ttttcacctc ctatcagggt
     3181 gacaagtgac aagtgataag tgataagaag accctgccgc cacatcttat cacttatcac
     3241 tcatcactca tcactcttcc cgttccacgc atcgataaaa atctccatcg tgccgccgca
     3301 gaccatgccc tcctcgctgg ccgcctcggc cgtgaggctg aatttgtgga gcgcggccgc
     3361 gcccgtccgc atcgtctcgc gggcctcggc ccagacctcg gcctcgatgc agccgccgcc
     3421 cacggtgccg gccacccgcc cgtcctcgaa gaagagcatc tttgcccccc gccgctgcgg
     3481 cgtcgagccg cgcgtgtgcg ccaccgtggc gacaacgacg cgctcgcccc gcgcattggc
     3541 ctcttctacc aaacggtaaa gctcaagctg ggaaagcata aaaaagcaga ggtcagaggt
     3601 tagaggttag aggtcagtga aagacggagt gattttcact gacctctaac ctctaacctc
     3661 tgacctctta ttcctgtggc ggctcggccg gggggttgct ggttggggtc ggcgtgggcg
     3721 cgggctgtgt gatggtggtc tggttgccgg ccgcattggc gtccgtttgc tgttgctcga
     3781 tggcgtcgat gcgggccttg atgcgctcgg cgtcctgttc ggcggcggcg acggccgact
     3841 ccagacgcac gcggctcgtg ttgaggagtt cgagctggcg cgagacgcgt tctttctcgc
     3901 gttcgaggct gaggcgaatc tgctcgcgga cattcgcggg gttgagcgag ccgataagga
     3961 gggagcggcg ctcgatggct ccggggtcaa gctcggcggt gatctgcgcg agtcgatcct
     4021 gaaggacgaa ctccttgtcc gtcacgtcgc gcaactgtga gcgcaaactt tcggcgcgct
     4081 gctcggcgcg cgtgaggcgt tcgagatcga cgaggacgcg ctgctcgccc ttcatggcgg
     4141 ttaattcgtc ggagaggcgc gtcacggtct gcgtgagccg gttgacgctg ttgcgcatct
     4201 cctcttcacg ccgttgctgc tcgtcggcct cctgagcggc ggcgcgtcgc gtcgtgcgac
     4261 tgttgcgccg gcgcggcggc gtgtcatcgt cctgcgcggc cacgtcctcg gcggtgctga
     4321 tgaccgacgg ctccgcgggg agcggcgtcg gggcgggtag cacgtcacca cgcgcgcgcg
     4381 ccggcgcggt ggcgcggcgc gagggccggc ggccacgctg ctgcggcagc gcctccgcca
     4441 cgaacattgt cgcggtcagg agcaagatga gcgcgaccgc gcccgacgtg agagacgttt
     4501 tgaacttcat cacaaaaacc tccactgccg ctgcacttac acgccgacgg cgcggcgtcg
     4561 ttccatcaac cgggctgaat cttgaagctg atgtccacgg cgcgcgccga gtgtgtgagc
     4621 gcgccgacgc tgatgagatc gacgccggcc tcggcgtagg cgcgcacgtt cgcgagcgtg
     4681 atgccgcccg acgactccag aaggatggca ggctcaagct cgcgcgcgat gaggacgagg
     4741 cgggtcgtct cctccggcgt ctggttgtcg agcagaagga tgtcggcccc ggcgcggatg
     4801 gcctcgcgca agtcgcgctc gctcgccacc tccacctcga ccttgtgcag atgcccgacc
     4861 gcgtcgcgcg cgcgctcgac ggccgtggcc actccgccgg cgagcgcgat gtggttatcc
     4921 ttgatgagca cgccgtcgtc gagtccgaag cggtggttgc gcccgccgcc ggcgagcacg
     4981 gcgtatttct caagcatccg caggccgggc gcggtcttgc gcgtgtccac gatctgcgcc
     5041 tcggtgcctt cgacggcctt gacgaactcc cgcgtcagcg tcgcgatccc tgagaggcgt
     5101 tgcaggaggt tgagggcgac gcgctcggcg ccgaggagca cgtcggcgaa gcccgcggtg
     5161 cgcgcgatgg ttttgccggc ctcgacctcg tcgccgtcgg aggcgaaggc ttcgagctgt
     5221 tgcccggagt cgagggtgga gaagacggcc tcggcggctt cgaggccggc gacgaccatc
     5281 ggctccttgg cgagaaactt cccgcgcgcg cgctgctgcc gcatcacggt cgcctgcgtc
     5341 gtgatgtcgc cccggcccaa atcttcactg aggaaagcgc tgatctgcga gaagagcgcg
     5401 ccctcgtcaa gccacttcat cctttaagtg cctccagcat ctgttcgagc gcgacgattc
     5461 gttgcctgat gtcctccgag cgttgccgaa gttcttcgac cttctcggcc ggcgcgcgct
     5521 ccacgaactg agggttggcg agctgagctt cgagccgctc caactccttc gacagctttt
     5581 cgatctcctt gccgagccgc gccgtctcct gcccgaagtc gatcaagcct tcgaacggca
     5641 cggccaactc ggccccgccc gccagcgccg cccgcgccga cgcacgcggc gcatcggcca
     5701 tcgattcttt aaacgacacc tcgctcgcgc gcacgagccg cttgatctgt gggaggcttg
     5761 cgtcaaagac tcgccccagt ccgtcgccac cggcgctgat gatgagccgc acctcttcgg
     5821 acggcttgac gttcaactcc gtgcgaatgt tgcgcacgcg cgagaccagc tcgatgacgg
     5881 cgcgcatctc gctttcggcg cgctcgtcga tgagcgactc gtcggcgcgc ggatattccg
     5941 tgagcatgac ggtcggctcc atgccgcgat aggccggatg cagcgagtcg gcgccgacgc
     6001 cgggaaggcg ctgccacagc tcctccgtaa taaacggcat gaaaggatgc aggagcctga
     6061 gcgccgtctc cagcacggtg acgacgcggc tgcgcgcggc gtcgcgcgcc ggtgtcggtt
     6121 cggacgccgt cacgtcctgc ttcgtgagtt cgatgtacca gtcgcagaag tcgtcccaga
     6181 agaaatgata gagactgtag acggcctcgt ggaactgata gccctcgacg gcccgtgaga
     6241 cttcgcgcgc cgtccggttc aactccgaca tgatccagcg gtcgtgcagc gcccatccgc
     6301 tcgggtcgcc ctcaaaacct ttgcccacct gcgcgccctc ggaattcatc aggcagaagc
     6361 gcgcggcgtt ccagattttg ttggcgaagt tgcggtagct ctcgacctgc tcgtcgcgcc
     6421 agcggctgtc ggtgccggtc gaggcgggcg aggaggccag cgtgatgcgc gtggcatcga
     6481 cgccgtactt ctcgaacacg tccatcgggt ctacggcgtc gctcggcttg ctcttcgaca
     6541 ttttcttgcc gtgcttgtcg agcacgaggc cggtgatgac cacgtcgcgg aacggcaagg
     6601 ggccgatgaa cttcgtgccc gtcatcacca tgcgcgacac ccacaggaag atgatctcgc
     6661 gcgcggtcac gagcacgtcg gtcgggtaga aggtcttgag gtcggcggaa gtctcctcgg
     6721 gccagccgag cgtcgagatg ggccagagcg cggaggagaa ccacgtgtcg agcacgtccg
     6781 ggtcctgcgt cagcttgtcg gcgccggccg cgacgcgcgc gtcttcctcg gagcgcgcga
     6841 cgtagacgtt gccctcctcg tcgtaccaag cggggatgcg atggccccac cagagctgac
     6901 gcgagagcgt ccagtcgtgc aagccttcga gccagtcggt gtagaccttc tcccacggca
     6961 cctctgggac aaagtgcggc tcgtgtctct ctctgattaa ttcaagcccc ttctcggcaa
     7021 gctcccgctg tttgcagaac cactggaggg agatcagagg ctcgatgacg gtgcgacagc
     7081 ggtcgcagcg cgagacggcg ccctcgtagt cggcgactcg ttccagcaga cccagctcct
     7141 cgaacttctg gacaactttc tcgcgcgcct cgtagcggtc gagtccggcg aactccgcgc
     7201 cggccgcctc ggtcatgcgc gcgtgcttgt cgatgacgac gatctgcggc aggttgtggc
     7261 gctcgccgat ctcgtagtca gtggggtcgt gcgcgggcgt gaccttgacg gcgccggtgc
     7321 cgaaggccgg gtcaacagcc gcgtcggcga cgatggggat ttcgcggccc gttagcggca
     7381 gcatgagcgt cgcgccgatg aggtcttggt aacgctcgtc gtcggggttg acggccacgg
     7441 ccgtgtcgcc gagcatcgtc tcggggcgcg tggtggcgac cgtgacgcgg cgctcgtcgc
     7501 tgcccttcac cggatactgc aagtgccaga gctggccctt ctgcgtctcc ttgtccacct
     7561 caaggtcgga gagcgccgtc tgatccttcg ggcaccagtt gatgagccgc tcgccacggt
     7621 agatgagtcc ctcgtcgtga cagcggacga agaactcgcg cacggcgcgc gacaaatcat
     7681 cgtccatcgt gaaacgctcg cgcgaccaat cgacgctcgc gccctcgcgc cgcatctgct
     7741 ccgtgatgcg cccgcccgac ctctgtttcc actcccacac gcgctcgatg aatttctccc
     7801 ggccgaggtc gtgtcggctg agtccctcct tcttcagctc gcgctcgacg acgatctgcg
     7861 tcgagatgcc ggcgtggtcg gtgccgatca cccagagcgt gcgcttgccc cgcatgcggt
     7921 ggtagcgcgc gagcacgtcc atgatggtgt gctggagggc gtggcccatg tggagcgacc
     7981 ccgtcacgtt cggcggcggg atgacgattg aatagacggg ggccgactcg tctcggttcg
     8041 cctcgggcgc gaagcagccg gcccgctccc agcgcgcgta gtgttgctcc tcggcctgcc
     8101 gggggtcgta agtcttcggt atctccatag gttgacaagt atagtaacaa ggcaaaagta
     8161 aaaaggaaaa aggcaaaagg gagacagccg tcgccatctc ccttttgcct ttttcctttt
     8221 tacttttgcc ttacttttct tcgtcgagcc gctgccggat gattcgctcg gccagcgcgg
     8281 ggacgacttc ccaggcaatc tcctgcacgg ccctctccga catcagctcg acgacgcgac
     8341 gcgcaatcgc gtcgatcatc tcgggcgaca actgccccgg cgtcagcgtc gaagcagcgg
     8401 cggcggtgtc caccgactcc agcgactcgt cacgcacctc gccgggcgca ctttcttctt
     8461 catgtgcgac ggccgcgtcg gagaaatccc acgaggatgg ggccgccgcc acgggcggac
     8521 gggcaacgtc gccttcgacg ctcaagtctt catccatccg caccggctcg tcggaggcga
     8581 cgacctgcgg ctcgacgaag tcgcgcatgg cggacacgtc ctgcgtcgca tacatgtctt
     8641 cacgcggagc cgactcggcc gaaaaatcgt agcccgcgtg ggcggcggcc ggcgcgtcgt
     8701 cgccgtaagt ttcgcccgag ccgtgcgcgg cctcggcgaa ggcgccggcc gtgtcaacct
     8761 gtggcacgaa ttggccgccg ccgtaagccg cgggcgcggc ggcggaacct gccgacgctg
     8821 gcggcggctc gtcaaagtcg aggtcgagga cgaagtcgtc catttcggcg ggcgcggatg
     8881 acggctcgtc ggccacgccg aggtcgagga gggattcgtc ggccgtggcg acagacgaag
     8941 cgcgcgacgc gaacggggaa ctcccggtct gagactcggc ggcaacctcg cgccccgcgg
     9001 cggcggcgta ggccgactcc tcgcggcgct cgaccgggcg cgcttccgcc acggcgtcgc
     9061 ccgcgaacgc ggcgccgaca tctgcaagtt cggtgtgcgc ggcctcgtca cgccggccga
     9121 agctctcggc tggcgtggcc tcgatcatct catcgtccat agcgaagtct gtcgcgaagg
     9181 cgccggcctg cggcgcgcgc ttcgcgtcag cgtcctgcgc gacgacggcg ctcgtcgtcg
     9241 cttcgcgcac gccggccgcg taagcgtcgg tcgcgtgctc gtgacggcgc tcgtcgtcca
     9301 cgcggcgctc gtcgtctcgc gacgcttggg cggcatcctt gccgccgagc agcgagccga
     9361 ctttgccgat caagtctttg atggattgga atggtttgct caagacttcg tccgcgccga
     9421 cgcggcgcgc ctccgcttcg ttgaatggtt caaatgcgcc cttgaggagg acgaccggga
     9481 catgatgcca gagcgcgtcc tgcttgaccc gctcgcacac ctcatagccg ctcatgcccg
     9541 gcatgtgcac gtccgcgagc aggacatcgg gcggccccga ctcctccatg atgcggatcg
     9601 cctcttcgcc cgtgccggcc gacacgactt ccataccctc gtcagtgaac gtgagagtca
     9661 cgaccttctg gatggtgagt gagtcgtctg cgagaagcag tttggggcgt cgtgtctgca
     9721 aaaaagtctt ctccaatggg gacgttccgc cggccggagg gggttttgat ggggggactt
     9781 ctctggagtc cgctcccgag gtggcggcgg ctgggggcgt gtggcgcggg cgcgggacga
     9841 caattccctg ctcccgtggg ccaaaattaa cctaacaccc gcgccgaagg cgagtcaaga
     9901 ctttttaact gcgggcctca tagcagtttg cacttgcgtg cgaggcaaac ggggcgagga
     9961 aagggaacac ggattaaacg gataaagacg gatgagcacg gatgcgatcc gtcctcatcc
    10021 gtcctatccg tgtccccctc gcgcggcgca tggaccgacg cacctatcta cacagtgcta
    10081 tcagcgcgtc atgcgccaga acggcgaagt gaaatccagc gacgcaaaga cagcctcaca
    10141 ctcttcagcc gtccgcctcc gccacagcgg ctccctgcgg tcggctccga accaggcgag
    10201 ggcgagacgc cagcacctct cgaccgggaa ggattcgccg cgcggcatgc gccactgcgc
    10261 gcaccagcgt tcgacgtgct cctccgaccg gaagagcagc atcgtctttc aggtgaagac
    10321 gacgttctcc caccagcgcg ccgccggcag cgcgaagtgg acgacgctgc ccggctcact
    10381 ctcgacgagg acgccgccct cgacgcgcag actcatccgt gtgccgcagc aaccgcacga
    10441 ggcttccacc tccgcgtcgc gcccgagcac cacggggaca ccgagggcat cccacgcgca
    10501 gggggcaaag tacgcgcgcc cgcccgatct caccacgaag ggcgtcggca cggccgagaa
    10561 gggcgcggcc atcaaaatct ctccgccccc ttcctgaagg acgagcacac gcgcgtcggc
    10621 gaggcgacgg aaggcggctc gcacttcatc ggtcgaacac gaaagcgcgg ccgccgccac
    10681 ggccgactga ggcggcacgc cctccgtcat agcgtgctcg tagacgtagc ggcgcacctc
    10741 cgcgtcgaag gcggcctgtt catctgtttc cgtcatctta tgcggctccg cggaaatgag
    10801 ttcggtgttg gcatgggcga gtcagccgct gatggttacg tcgtcggccg ttgcgggcag
    10861 gccgtcgggg ccgtcggagc cgagcgtgaa gcgttcggtg gtgcccgcgt agcggtaggg
    10921 gcggtgccag gggtcgatgc ggatgaccgt cttgaggtag cgcgggctga ggtggtcgat
    10981 gaggacggtc tcgtcttcgg cgacgacata gaagccccgc tcgcgacggt aggcttcaag
    11041 ggccgcgcgc accgcttcga ggtcggcgcg cgcccgcacc gccttccgcg cgttgacgcc
    11101 cgcgatcaaa gcgtcgaagt caccgaacga tgcttcgccg acgctaaacc cgacgacgcg
    11161 ccacttgccg cgcgtgtcct tctccagacg gaacgaggcg tccaccacga cgacggcccg
    11221 cgccgacttg tacatcggcg cgaactcctt catcacgagc ggcccccggc gcacttcgag
    11281 cttcttctct tcttcggcct tctcgtcctt cgtctttccc tcctccgact tctctttctc
    11341 cagcttgtta tctttcgact tctcgccctt tcccgccgct tcttccgcgg ccttgcgctc
    11401 gcgctgttcg gtctcgaact cggcggcgag ggcttcgagt tccaagcgcg ctgcctcgac
    11461 ccgttcgacg cccagcgggc cggcgagata gtcgaactct tcccacgcgc ggtcgcccgt
    11521 gcggaactcg acggcgcgcc agcgcgcgcc ggccccgccg gcttccgtct tcgcgaagcc
    11581 gaacgcgatc tcgacctcgg cgttgacgac gacgggcgtc gcgcccgcga gcgacacgtc
    11641 tttcacacgc acccggcccg tgcgaaggtc gaagccgggc gtggcggcga tcaccttccg
    11701 cgcgtcgcta tccttgatgg ccttc
//
LOCUS       DASVES010000134         8290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1386990, whole genome shotgun sequence.
ACCESSION   DASVES010000134 DASVES010000000
VERSION     DASVES010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386990"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VF240_04840"
     CDS             <1..472
                     /locus_tag="VF240_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7174592.1"
                     /translation="RELLARTRPRSIGWMSVENPYGMSNTEFVVRPDAAPRAEMGCPH
                     FAGIFAIGAAAEYFQRLGLERIERRVLALNRYLTERLADAGWRVLSPTRREGARSAET
                     LVAMAEPRRTVAQLARRGVAVTLKPEGIRVATHFFNDETDIDRLIDALGEMKRP"
     gene            1043..1114
                     /locus_tag="VF240_04845"
     tRNA            1043..1114
                     /locus_tag="VF240_04845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1075..1077,aa:Gly,seq:ccc)
     gene            1286..1945
                     /locus_tag="VF240_04850"
     CDS             1286..1945
                     /locus_tag="VF240_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174593.1"
                     /translation="MRKYLLTGICALALLAGTASQTAAQQDTVKKTADKTVEKTKDVG
                     QEVGDKAEDAKDQTVKGAKTVGRKTKDAGKEAADKTEDAAGKVKDGAAEVGDKAEDVG
                     DQTVKGAKAVGSKAADKAEDVGDKAVDVTKKGVNEVGDKAEDVGDKAAKGARVAGRGA
                     KAVGAEAADKAEDAGDATVKGAKAVGHEVADKAEDAGDQSAKGAKKTGNWFKRAFRKI
                     F"
     gene            2258..2554
                     /locus_tag="VF240_04855"
     CDS             2258..2554
                     /locus_tag="VF240_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174594.1"
                     /translation="MTETKSWTVICPCCEATLVVDAQTGALISHEEKAKPLASFEEMA
                     KQMERQKQLREQVFSQELGSQKDRERILEEKFREAFKRAEGEKDKPYINPLDMD"
     gene            complement(2673..2891)
                     /locus_tag="VF240_04860"
     CDS             complement(2673..2891)
                     /locus_tag="VF240_04860"
                     /inference="COORDINATES: protein motif:HMM:NF040111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HEX7174595.1"
                     /translation="MGSDKDVEKSYSNKEVVAKLRRLADALEEEKTFEIQIAGERVYV
                     PPYATVEFEYAKEGDEEEVEIELRWKKQ"
     gene            complement(3120..5189)
                     /locus_tag="VF240_04865"
     CDS             complement(3120..5189)
                     /locus_tag="VF240_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX7174596.1"
                     /translation="MESPAGDSRSDTPKTTHPPARSRRERDEDTQVERKGRRRLDAHP
                     DRIDIRDMFYNPSLAPLPDQIVNIDNVPAILDQGQEGACCGFALAAVINFLLHSRGLG
                     RLVSPRMLYEMARRYDQWPRENYSGSSARGAMKGWLAHGVCESETWPPSRHGARHLTP
                     DIAREGQNTPGGAYFRILHRQIRDMHAALGEVGIIYCTIQVHDGWSNFGDDYVKLDGV
                     KNPLVAKQGIPVIKRVDGPTSGHAIALVGYTSEGFIVQNSWGDTWGAGGFALLPYEDY
                     MLHATDVWVAQVGVPVRLSIWKHPELQPEAQTEGLQGSASVVPLSDIRPYTVDVGPDG
                     QLSTSGRYWTTEQDIERLFTDSIPEAAKDWKKRRLMLYLHSGFQNEDRLAQRIIAFRD
                     VMLKNEVYPVNIMWESGVMDSWQKLLKETLVGERDEGKTADWLRRVRGGSLDVRDDTF
                     ELTVASRGSQLWREVKENARLASDSPDGRGAMQLLVEHAKTALGKVSANRRESWELHV
                     VAHSIGSIFAAHAVRHLAGLGVGFKSLHLMAPAMTVNLFREEVLPFVEEGACPHPSLY
                     IQSEVGERDDDVGPYGKSLLFLISNAFEGDRNTPLLGMQRFVSTGGDTRSEHTDMEMN
                     RLFRKSVDGEPSLVIAGRDGGPRSTSRAESHTGFDTDVDTLNSILRRIVGSNKPKRAF
                     KIRDLQY"
     gene            complement(5375..6676)
                     /gene="hemL"
                     /locus_tag="VF240_04870"
     CDS             complement(5375..6676)
                     /gene="hemL"
                     /locus_tag="VF240_04870"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX7174597.1"
                     /translation="MDATVEKKAGRSEELFERAVRLMPGGVNSPVRAFRGVGGVPRFI
                     ASARGALMTDADGREYIDYVGSWGPMILGHADEEVVESLREALGRGTSYGAPTELEVL
                     MAEEIIDAVPSVEMVRMVNSGTEATMSALRLARGVTGREKIVKFEGCYHGHADSLLVR
                     AGSGVATLGLPDSPGVTPAVAANTITAPFNDADAIAKIMSEHGDVAAVIVEPVAGNMG
                     CVPPRAGYLQHLRDLTKKHGALLIFDEVMTGFRVARGGAQAFYNVTPDITALGKIIGG
                     GLPVGAYGGRRDIMEHVAPAGAVYQAGTLSGNPLAMTAGLVTLRRLRDSAVYDQLERA
                     TRRLCDGLTAAAREAGVETQTNRVGSMFTTFFTGRPVFDFASASESSRTRYAAFFHAM
                     LREGVYLAPSQFEAAFVGLAHTDELIDRTVEAAGRAFRAFA"
     gene            complement(6734..7918)
                     /locus_tag="VF240_04875"
     CDS             complement(6734..7918)
                     /locus_tag="VF240_04875"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX7174598.1"
                     /translation="MGSTLFESVEQTTAGLLDAVADAVVGEASTLRDGTEHAGVLLPR
                     DLYAHNEAQTEWWYYTGHLRTESGRQLGFELVFFKRRTDLDRFGVVPLRLIANPLYLA
                     HFAMTDETGRRFRYDHRKSANGPFDHPALTSARRFYLKLGDWTVREAHGLHLLHATLG
                     DDLIFDAALSPTKPAALNGHERAGVSFKDHGEASRYFSYTRMAAEGDITFGGRTEHFT
                     GTAWMDREFGTWTTTDDQKGWDWFSIQLDTGSELMVYHIRDREGRPSSFSSGTYVDAS
                     GDWTHLARADFQLEVLDHWRSPHTGALYPSRWRLRVPRFRVDVTVSPVLNDQELDTRG
                     TTMIVYWEGACAVVGQHEDRDTRGRAYVELVGYDRSHLQPSLTTFLFGAALDRRWRSI
                     FG"
     gene            complement(8097..>8290)
                     /locus_tag="VF240_04880"
     CDS             complement(8097..>8290)
                     /locus_tag="VF240_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174599.1"
                     /translation="HRTVIEAAQLAAHFSRAGKDAKVAVNYTERKYVSKIKGGAPGLV
                     RLSSFRTLLVEPGEELERI"
BASE COUNT         1476 a         2510 c         2827 g         1477 t
ORIGIN      
        1 ccgcgaattg ctcgcgcgca cgcggccgcg ctccatcggc tggatgagcg tcgagaatcc
       61 ttacgggatg agcaacacgg agttcgtcgt gcgccctgac gccgccccgc gcgccgagat
      121 gggctgcccg cacttcgccg gtatcttcgc catcggcgcg gccgcggaat actttcagcg
      181 tctcggcctc gaacgaatcg agcgccgcgt cctcgccctc aacagatatc tgaccgaacg
      241 tctcgccgac gccggctggc gcgtgctctc gcccacgcgc cgcgagggcg cgcgctcagc
      301 cgaaaccctc gtcgcgatgg ctgagccgcg ccggacggtc gcccaactcg cgcgccgcgg
      361 cgtcgccgtc acgctcaagc ccgaaggcat ccgcgtcgcc acacacttct tcaacgacga
      421 gacggacatc gaccggctga tcgacgcgct cggcgaaatg aagcgcccgt gacttgacct
      481 gtcgataagc aggtaacgca ggcagttcga atgcttgatg aagtgttaat tgacaccgtt
      541 ccagacgctc aggtatcttt cccgatcaaa cgcatcggcg gggtatttat cgcacgtgcc
      601 cgcgtgccag caactcaact gtagagcatc aacttcatgc ggaccgggga gaagggaaat
      661 ctgagcgagg ctagaatcct ggctgccttt gtggctgccg gatacttcgt gtccattccc
      721 tttggctcag ggcacaagta tgatttcgta gtcgatgatt cgactcgttt gttccgggta
      781 caatgcaaga cgggtcgagt caaaaacgga tcgcttattt ttaacgctta cagtcagtcg
      841 ggcaacggag cggttaagat gagttatcga ggactggcgg acttgtttgc cgttctcaaa
      901 cctgaagatg ataaggtata cttaattcca gttgatgaag ttggggtgac ggatgtttca
      961 ttgcggctcg ctccgacctt gaacaagcag gcacagggga tacggtgggc agagatttat
     1021 ctgctgaagc cgttagttct aagcgggagt ggcgcaatgg tagcgcatta gcttcccaag
     1081 ctaagggttg cgggttcgag tcccgtctcc cgcttttaat ttctgacaga gcaaagctct
     1141 cacggccgcc tccacgggcg gccgttcttg cgttcggatg tgtgcatagt ttgcctccgt
     1201 cccgcctctg gcatcgcgct tgccactctt ctccacaggc gttggggact gcttcatttc
     1261 attacaccta ggaggtggca tccgaatgag aaagtatttg ttaacaggca tttgcgcgct
     1321 tgctcttctg gccggtacag cgtcacaaac tgcggcacag caggacaccg tcaagaagac
     1381 ggccgacaag accgtcgaga agacgaagga cgtgggacaa gaggtcgggg acaaggcgga
     1441 ggacgcgaag gatcagacgg tgaagggcgc caagaccgtc gggcgcaaga cgaaagacgc
     1501 gggcaaggaa gcggctgaca agaccgagga cgccgccggc aaggttaagg acggcgccgc
     1561 ggaagtcggc gacaaggccg aggacgttgg cgatcagacc gtgaagggcg cgaaggctgt
     1621 cggcagcaag gcggctgata aggccgaaga cgttggcgac aaggccgtcg atgtgaccaa
     1681 aaagggcgtc aacgaggttg gcgacaaggc cgaggacgtg ggagacaaag cggccaaagg
     1741 cgcgagggta gcgggccgcg gggcgaaggc cgtgggcgcg gaagctgctg ataaggccga
     1801 ggacgccggt gacgcgaccg tcaagggcgc aaaggccgtc ggccacgaag tcgcggacaa
     1861 ggccgaggac gcgggcgacc agagcgcgaa gggcgccaag aagaccggca actggttcaa
     1921 gcgggcgttc agaaaaatct tctaacgaag ttaacaactg aaatccgttc gcgaccgcgg
     1981 cgccggtgac ggccgaaccc gcgcgtcgcg agcggattgc gttttgtcgg ggatgtgaaa
     2041 agtaaatccg gggttggacg ccgtcacggc ctgacttctt ttccaaacgc ttcgacgcaa
     2101 aaggcggccc attgctgggt cgccttattt tttgcccggc gaaatttatc gagcttcggc
     2161 ccgccgccgg gcaaggtgct atactcgtcg cgcaagcgcg ccaacgggtg tctttcaagc
     2221 gttgagaatc ctttagacac ttgcggagga gagacaggtg accgagacaa agagttggac
     2281 tgtgatctgc ccctgctgtg aggcgacgtt agtggtcgat gcgcagaccg gggcgctcat
     2341 ctcgcacgag gagaaggcca agcccctcgc gtcgttcgag gagatggcca agcagatgga
     2401 gcgccagaag cagctccgcg agcaggtctt ctctcaggag cttggctcgc agaaggatcg
     2461 cgagcgcatc ctcgaagaga agttccgcga ggcgttcaag cgcgccgagg gcgagaagga
     2521 caagccctac atcaacccgc tcgacatgga ctagaggcca cgccccgtgc ggcgcatctc
     2581 taccgacgaa accgaaacgg taactacaag cgccgcccgc gtagcctctt gtcatcgttc
     2641 acttcgcgcc gcatcgagcg cggccccgct cttcactgct tcttccagcg cagctcgatc
     2701 tcgacctctt cctcgtctcc ctctttcgca tactcgaact cgacggtcgc gtaaggcgga
     2761 acatagacgc gctcgcccgc gatttgaatt tcaaaagtct tctcttcttc cagtgcgtcg
     2821 gccaaccgcc gcagcttggc gaccacttcc ttgttcgagt aagacttctc cacatcctta
     2881 tcactgccca tcgttcacct cccgagaatt aaagctgtat cgaactaatc gaactagaac
     2941 gacttttact tgagtgaaag ggaaatagat tcaccgcaag aggcgcggag ggcgcggagt
     3001 tcgcgccgag ggttgaatca ctctctgcgt gaactctgcg ccccctgcgc ctccgcggtg
     3061 aatctacttg ggctatactc aagtgaaatt agatagcgtg cgtgcgccgc ccgccccttt
     3121 cagtattgca ggtcgcgaat cttaaaggcg cgtttcggct tgttgctgcc gacgatgcgc
     3181 cggaggatgg agttgagcgt gtccacgtcg gtgtcgaaac cggtgtgcga ctcggcgcgg
     3241 gaggtgctgc gtgggccgcc gtcacggcct gcgatgacga ggctcggctc gccgtccacg
     3301 ctcttgcgga agagccggtt catttccatg tccgtgtgct cgctcctggt gtcgccgccg
     3361 gtgctgacga agcgctgcat gccgagcagc ggcgtgttgc ggtcgccctc gaaggcgttg
     3421 ctgatgagga agaggagcga tttgccgtag gggccgacat cgtcgtcgcg ctcgccgacc
     3481 tcgctctgga tgtagagcga cgggtgaggg caggcgcctt cctcgacgaa cgggaggact
     3541 tcttcgcgga agagattgac cgtcatggcc ggggccatca ggtgcaggct cttgaaaccg
     3601 acgccgaggc cggcgagatg ccggacggcg tgggccgcga agatcgagcc gatgctgtgg
     3661 gcgacgacgt gcagctccca cgactcgcgc cggttcgccg agaccttgcc gagcgcggtc
     3721 ttcgcgtgct cgacgaggag ctgcatggcg ccgcggccgt ccggcgagtc cgaggcgagc
     3781 cgcgcgttct ccttcacctc gcgccagagc tggctgccgc gggaggccac cgtcagctcg
     3841 aaggtgtcgt cgcgcacgtc gagcgagccg ccgcgcacgc gccgcagcca gtcggccgtc
     3901 ttgccctcgt cgcgctcgcc gacgagggtc tctttcagga gcttctgcca cgagtccatc
     3961 acgcccgact cccacatgat gttgacgggg tagacctcgt tcttcagcat cacgtcgcgg
     4021 aaggcgatga tgcgttgggc gaggcggtct tcgttctgga agccgctgtg gaggtagagc
     4081 atcaagcggc gcttcttcca gtccttggcc gcctcgggga tggagtcggt gaagaggcgt
     4141 tcgatatcct gctcggtcgt ccagtagcgg ccggaggtgg agagctggcc gtcggggccg
     4201 acatccacgg tgtagggtcg gatgtcgctt aaggggacga cgctggccga cccctgaagt
     4261 ccctcggtct gggcctcggg ttgtagctcc gggtgcttcc agatgctcag gcggacgggg
     4321 acgccaacct gtgcgaccca cacgtcggtg gcgtggagca tgtagtcttc gtaggggagg
     4381 agcgcgaagc cgcccgcgcc ccacgtgtcg ccccaggagt tttggacgat gaagccctcg
     4441 ctggtatagc cgacgagggc gatggcgtgt ccagacgtcg ggccgtcaac gcgcttgatg
     4501 acggggatgc cctgcttggc gacgagcggg tttttgacgc cgtcgagttt cacgtaatcg
     4561 tcgccgaagt tggaccagcc atcatgcacc tggatggtgc agtagatgat gccgacctcg
     4621 cccagagctg cgtgcatgtc gcgaatctgg cggtgcagga tgcggaagta agcgccgccg
     4681 ggcgtgtttt gcccctcgcg cgcgatgtcg ggggtgaggt ggcgcgcgcc gtggcgcgac
     4741 ggtggccacg tctctgactc gcagacgccg tgcgccaacc agcctttcat cgcgccgcgc
     4801 gccgaggagc cggaataatt ctcgcgcggc cactggtcgt agcggcgggc catctcgtag
     4861 agcatgcgcg ggctgacgag ccggccgagt ccgcgcgagt ggaggaggaa gttgatgacg
     4921 gcggcgagcg cgaacccgca gcaggcgccc tcctgcccct ggtcgaggat ggccggcacg
     4981 ttgtcgatgt tgacgatctg atctgggagt ggtgcaagcg acgggttgta gaacatgtcg
     5041 cggatgtcga tgcggtcggg gtgcgcgtcg aggcgacgcc gacctttgcg ctcgacctgc
     5101 gtatcttcgt ccctctcgcg ccgtgaacgc gcgggcgggt gtgtggtctt cggcgtgtcg
     5161 ctgcgggagt cgcccgcggg ggattccaat acggtgcgct gactgcgctg actcttcttg
     5221 ctggacggca tgagaattcc tttcggcgcg cgggggcggt tggactgagg aaggcggctc
     5281 gcactgcccg cgcgcgcggg cggtgcgaca aatggccctt cagaaagcgt ctgcgcttat
     5341 atcttcgccg gctcggggct gtcaacagga aagttcaggc aaaggcgcgg aaggcgcggc
     5401 cggcggcctc gaccgtgcgg tcgatcaact cgtccgtgtg cgccagaccg acgaaggcgg
     5461 cctcgaactg cgacggcgcg agatagacgc cctcgcgcag catggcgtgg aagaaggcgg
     5521 catagcgcgt gcggctcgac tcggaggccg aagcgaagtc gaagacgggc cgacccgtga
     5581 agaaggtcgt gaacatcgac ccgacgcggt tggtctgtgt ctcgacgccg gcctcgcgcg
     5641 cggccgctgt cagtccgtcg cagagacggc gcgtggcccg ctccagttga tcataaaccg
     5701 cagagtcgcg cagtcggcgt agggtgacga ggccggcggt catcgccagc gggttgccgg
     5761 aaagcgtgcc ggcctgatag accgcgcccg ccggtgcgac gtgctccatg atgtcgcgcc
     5821 gcccgccgta agcgccgacg ggcaggccgc cgccgatgat cttgccgagc gcggtgatgt
     5881 cgggcgtaac attgtagaat gcttgcgcgc cgccgcgcgc cacgcgaaag ccggtcatca
     5941 cctcgtcgaa gatgagcagg gcgccgtgct tcttcgtcag gtcgcgcagg tgttggaggt
     6001 agcccgcgcg cggggggacg cagcccatgt tgccggcgac cggctcgacg atgacggcgg
     6061 ccacgtcgcc gtgctccgac atgatcttcg cgatggcgtc ggcgtcgttg aagggggcgg
     6121 tgatggtgtt tgcggcgacg gccggcgtga cgccggggct gtcgggcagg ccgagcgtgg
     6181 cgacgcccga gccggcgcgc accagcaggc tatcggcgtg gccgtgatag cagccctcga
     6241 acttgacgat cttttcgcgg cccgtgacgc cgcgcgcgag ccgcagcgcc gacatcgtcg
     6301 cctccgtccc gctgttgacc atcctgacca tctcgaccga cgggacggcg tcgatgatct
     6361 cctcggccat caacacctca agctcggtcg gcgcgccgta gcttgtcccg cgccccaaag
     6421 cctcgcgcaa cgactcgacg acttcctcgt ccgcgtggcc gagaatcatc ggcccccacg
     6481 agccgacgta gtcgatgtac tcgcgcccgt ccgcgtccgt catcaacgcg ccgcgcgccg
     6541 aagcgatgaa gcgcggcacg ccgcccacac cgcgaaacgc gcgcacgggc gaattcaccc
     6601 cgcccggcat caaccggacg gcgcgctcga agagttcttc cgagcggccg gctttctttt
     6661 caacagtagc gtccatgtct caatgctaga cgaaaaacga gaaaggcgta aatcgaccat
     6721 cgtgaatcgg ccgtcaaccg aagatgctgc gccagcgtcg gtcaagggct gcgccgaaga
     6781 ggaaggtggt gagcgagggc tgcaagtggg agcggtcgta gccgacgagt tcgacgtagg
     6841 cgcggccccg cgtgtcgcgg tcttcgtgtt ggccaacgac ggcgcaggcg ccctcccagt
     6901 agacgatcat cgtcgtgccg cgcgtgtcca actcctgatc gttgaggacg gggctgaccg
     6961 tcacgtcaac acggaagcgg ggcacgcgca gacgccagcg gctcgggtag agggcgccgg
     7021 tgtgtgggct gcgccagtgg tcgagcactt cgagttggaa gtccgcgcga gcaagatgcg
     7081 tccagtcgcc ggaggcgtca acgtaggtgc cgctggagaa agatgacggg cggccttcgc
     7141 ggtcgcggat gtggtagacc atcagctcgg agccggtgtc gagctggatg ctgaaccagt
     7201 cccagccctt ctggtcgtcg gtggtcgtcc acgtcccgaa ctctcgatcc atccacgccg
     7261 tgcccgtgaa gtgctcggtt cgaccgccaa aggtgatgtc gccctcggcc gccatgcgcg
     7321 tgtaggagaa gtagcgcgaa gcctcgccgt gatccttgaa gctcacgccg gcgcgctcgt
     7381 ggccgttgag cgcggcgggt ttggtggggc tgagcgcggc gtcgaagatg aggtcgtcgc
     7441 cgagcgtggc gtggagcagg tgcaggccgt gggcctcgcg caccgtccag tcgccgagct
     7501 tgagatagaa gcgccgggcg ctggtgagcg cggggtggtc gaaggggccg ttagcgctct
     7561 tgcgatggtc gtagcggaag cgtcggccgg tctcgtccgt catggcgaag tgggcgaggt
     7621 agagcgggtt ggcgatgaga cgcaggggga cgacgccgaa gcggtcgagg tcggtgcgac
     7681 gcttgaagaa gaccagctcg aagccgagct gtcggccgct ttcggttcgc aggtgcccgg
     7741 tgtagtacca ccactcggtc tgcgcctcgt tgtgcgcgta gaggtcgcgc ggcaggagca
     7801 cgccggcgtg ctccgtcccg tcgcgcaaag tggacgcctc gcccacgacg gcgtcggcga
     7861 ccgcgtcgag taagcccgcg gtcgtctgct caaccgactc gaaaagagtg ctgcccatca
     7921 atcgttaacc cccctgatag ttgtcgcaaa gttgtctgat gaatatacgc gacttggcgg
     7981 cgccaaaaca gtaccgcgag cagtagcgag cgggtgtgac tcacgcgagg cgggtctctc
     8041 gttacggtca cacccgctcg ctaccgctcg cagtactgtg ctgctccgag gcgcacttaa
     8101 atcctttcca gctcttcccc cggctcgacg agcagcgtgc ggaagctcga caggcgaacg
     8161 aggcccggcg ctccgccctt gatcttcgag acgtacttgc gctcggtgta gttgacggcc
     8221 accttcgcgt ccttgcccgc gcggctgaag tgtgcggcta actgcgcggc ctcgatgacg
     8281 gtgcggtggg
//
LOCUS       DASVES010000135         7398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_687439, whole genome shotgun sequence.
ACCESSION   DASVES010000135 DASVES010000000
VERSION     DASVES010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687439"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VF240_04885"
     CDS             <1..820
                     /locus_tag="VF240_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7174600.1"
                     /translation="GPFALGASLGPLARRVEDLALLFGVLTGEDLARQSEDTGDALLK
                     DEAALLRGRRVAWYADDGATPVIPEIARAVEAAARALEDAGMFAVERRPPHVERATEL
                     WLGLFSYPTQEFLRRTYAGREEDAGSVARLLLERAERASAPTVNAYFELWDERDRLRG
                     ELLEWMEETPLVVAPVGAVAAPAHGARRVSAGGAEFGMFRAFAYAQAFNVFDLPAACV
                     PAGLTEEGLPVGVQIVGRPHAEREVLAAARIVEEALGGWQGVPGKHSQAGANPL"
     gene            857..1873
                     /locus_tag="VF240_04890"
     CDS             857..1873
                     /locus_tag="VF240_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7174601.1"
                     /translation="MFLILLAFLAIAALVVAAKTIKIVPQSTALLIERLGRFNRLAAS
                     GLNVIIPFLDKPRGVYWTNVRPGLASIDLREQYIDLPPQPVITRDNVTIHVDSVVYWQ
                     ITDPIKAVYEVTDLIGGLVQLTITGMRAVMGDMDLDHTLSSRDQINAKLRIILDEATD
                     KWGVKVTRVDVKNITPPEDVRLTMEKQMTAERNRRALVLQAEGDKQANITRAEGEKQA
                     AVTRAEGEKQSAILQAEGAAQARLRSAEAEAQAIRQVAGSIEGMGNPAQYLITARYIE
                     SLKEMTRGQNAKVIFMPMETAGVLSSVGAIKEMFAETGERRGKQDEEGGERGGRMPPR
                     QLPT"
     gene            complement(1878..2468)
                     /gene="thpR"
                     /locus_tag="VF240_04895"
     CDS             complement(1878..2468)
                     /gene="thpR"
                     /locus_tag="VF240_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX7174602.1"
                     /translation="MTSASDKNLRVFCAVELSAEVRELVGDYIAKLREMFPNVRASWE
                     RPEKLHLTLKFYGDIKPARVDQLTGAAERAAARISTFPIKLSGAGAFPPKGPARVLWL
                     GVVDESGGLASLQRHIEDECSTEGFPREARPYSPHLTLARMRTPAGVRPLAEFHNASS
                     FESEPFTVSEFVVMRSELLPTGSRHTPLSHHPLSQP"
     gene            complement(2501..4954)
                     /locus_tag="VF240_04900"
     CDS             complement(2501..4954)
                     /locus_tag="VF240_04900"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX7174603.1"
                     /translation="MKRFSRGARATLALLFTLSIFTLLPSDSLRPRANAQAQSQTVRL
                     AQLKRPVTVRRDERGIPYIEAENDEDLFFVQGYVTASDRLWQMDLLRRTARGELSEIF
                     GRATLEEDKRRRTLGFARVAEQSADAATPQARAAVEAYARGVNAYIDSLDAKSLPPEF
                     QILGYRPRHWTPADSNVVGKNFAEALSTTWRIDLMRAMLADLPPEKLRELLPVASPLD
                     VLVVGTDAKAAPRRTGAGRTRSAVPVDSIDKDAWLRALAVEDSIFRRTQERAGLFAED
                     LAASNNWVISGRHTATGKPLLANDPHLAPSAPPIWYMVHLSAPGLRVAGVTAAGAPGV
                     IIGHNERIAWGLTNLGPDVQDLYLEKFDPANPRRYLTPAGWSEAEVRREEIKVRKSPA
                     DASTETVTHEVTVTRHGPVIFERDGARYSLRWTALDPRSNEFEGFRLLNRARNWNDFR
                     AALSKYEGPVQNFVYADVDGHIGYYGAGRIPLRKTGDGSVPYDGSTDEGEWTGVIPFN
                     ELPHVYDPPSGIIVTANSRIVGTSYPHHLSHVWAPPTRARRIQQMLTAKKKLTVEDFR
                     AVQGDTFSISGDNFAREVVRIARGAALDRRDEKWRSTVQLLADWDGRLEPDSRAALLV
                     YEMRAQFARKIVEGAVGAERAKEFRWSNSATLIDRLIATRPQGWLPTSFKTYPELIDE
                     IHRSARASLSGRLGADEAAWTWGRDSQMRFPHPLAGVPFVGQQFAVQPLPQRGNPGGM
                     ASVNVGAAVSMRLIADTADWDRTQHAVTLGQSGLPSSPHFKDQLADWQNVTPRAFPFT
                     PRAVSAAARETRQFTPARP"
     gene            complement(5149..6573)
                     /locus_tag="VF240_04905"
     CDS             complement(5149..6573)
                     /locus_tag="VF240_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7174604.1"
                     /translation="MEAKPFLIGGKWRTSSRTQEVRSPYNGELVANFSVASAEELEEA
                     ISSATVAAREMRALARHVVAESLRHIADRLRERREEFARTIALEAGKPIKAARAETER
                     AVATFTYASEEARRFKGETVALDGQPGGVGRTGWTERVPRGVIYGITPFNFPLNLVAH
                     KVAPALASRNAIIVKPSPRTPLTALLLGEVFLESGLPAAALQVVPVDVPDIDVALKDE
                     RVAMISFTGSVEVGWRLREHAGRKAVALELGGNAPVIVDETADVEFAAERTAAAAFAY
                     AGQVCISAQRVFVHEQVAERFNELFVGRARAMRAGDPLDEATEISNMIDEAAARRVEG
                     WINEAVGAGARLLCGGRRDGSLLEATVLADVHAEMRVCSEEVFAPVATVQTFADFGEA
                     LDEANNTRYGLQAGVFTRDMARALRAFRELEFGGVLVNDAPNFRVDNMPYGGVKMSGA
                     GREGVRYAMEEMTEPRLIVIDPRL"
     gene            complement(6874..>7398)
                     /locus_tag="VF240_04910"
     CDS             complement(6874..>7398)
                     /locus_tag="VF240_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX7174605.1"
                     /translation="RRPLAGEEGVGVLRKVLRMLAVAVALVLCGIQFIRPERETPARA
                     SAQPIEQHVIVPPEVNQVLTRACKDCHSNETNWPWYSHVSPVSWFVAEHVNDGRSELN
                     FSDWGDYTPHDSSEILEMVCREVKRETMPLKSYLILHPEARLTPADVKTLCDWSDTEG
                     RRLAATSGAASSRQ"
BASE COUNT         1237 a         2331 c         2587 g         1243 t
ORIGIN      
        1 cggcccgttc gccctcggcg cgagcctcgg ccctctcgca cgtcgagttg aagacctcgc
       61 actgttgttc ggcgtgctga cgggggaaga cttggcgcgc cagagcgaag acacagggga
      121 cgctctattg aaagacgagg cggcgctttt gcgtggccgg cgcgtggcgt ggtatgcgga
      181 cgacggcgcg acgcccgtca tccccgagat tgcgcgtgcg gtcgaagcgg cggcgcgtgc
      241 gcttgaggac gcgggaatgt ttgcggtgga acgccgacct ccgcacgtcg agcgcgcaac
      301 cgaattgtgg ctcgggcttt tttcgtaccc cacgcaggag ttcctgcgcc ggacttatgc
      361 ggggcgcgaa gaagatgccg gctccgtcgc gcgtctgttg ttggaacgtg cagagcgggc
      421 gagtgcgccg acggtgaacg catacttcga gttgtgggat gagcgcgacc gtctgcgcgg
      481 cgaactgctc gaatggatgg aagagacgcc gctcgtcgtc gcccccgtcg gggcggtggc
      541 cgcgccagcg cacggcgcgc ggcgtgtctc ggcgggcggc gcggagttcg gaatgtttcg
      601 cgccttcgct tacgcgcaag ccttcaacgt cttcgacctg ccggccgcgt gcgtacccgc
      661 cgggctgacg gaggaggggc tgccggtggg cgtgcagatt gtcggtcgtc cgcacgcgga
      721 gagggaagtg ctcgccgcgg cgcgcatcgt cgaggaggcg ctgggcggat ggcagggcgt
      781 gcccggcaaa cattcacagg cgggcgcgaa tcctttatag tctcggacga acgcataact
      841 tcggaggacg ctcaacatgt ttctcatcct tctggccttc ctcgctatcg ccgcgcttgt
      901 cgtcgccgcc aagaccatca agatcgtccc gcaatcgacc gccctgctca tcgagcgcct
      961 cggccgcttc aaccgcctcg cggccagcgg gctaaacgtc atcatccctt tcctcgacaa
     1021 gccgcgcggc gtctactgga cgaacgtccg gcccggtctg gcctccatcg acctgcgcga
     1081 gcagtacatc gacctcccgc cgcaacccgt catcacacgc gacaacgtga ccatccacgt
     1141 cgattcggtc gtctactggc agatcaccga cccgataaag gccgtctacg aagtgaccga
     1201 cctgatcggc ggcctcgtgc agttgacgat cacgggcatg cgcgccgtga tgggcgacat
     1261 ggatttggat cacacgctct cctcgcgcga ccagatcaac gccaagctgc ggattattct
     1321 cgacgaggcg acggacaagt ggggcgtgaa ggtgacgcgc gtggacgtga agaacatcac
     1381 gccgcccgag gacgtgcgtc tgacgatgga gaagcagatg acggccgagc gcaaccgccg
     1441 cgcgctggtg ctgcaagccg agggcgacaa gcaggcaaac atcacgcgcg ccgagggcga
     1501 gaagcaggcc gccgtgacgc gcgccgaggg tgagaagcag tcggccatcc ttcaggccga
     1561 gggcgcggcg caggcccgcc tgcgctcggc ggaggccgag gcccaagcca tccgacaggt
     1621 cgccggctcc atcgagggca tgggcaaccc cgcccagtat ctcatcacgg cgcgctacat
     1681 cgagagtctc aaggaaatga cgcgaggcca gaacgccaag gtcatcttca tgccgatgga
     1741 gacggcgggc gtgctctcca gcgtcggcgc catcaaggag atgttcgcgg agacgggcga
     1801 gcgccgcggc aagcaggatg aagaaggcgg cgagagaggc ggccgcatgc ctccgcgtca
     1861 acttccgacg tagattttca aggctggctt aacgggtgat gcgacagtgg ggtgtgacgt
     1921 gagccggtcg gcaaaagctc gctacgcatg acgacgaact cggagacggt aaaaggctcc
     1981 gactcgaacg acgacgcatt gtgaaactcg gcgagggggc gcacgccggc gggcgtccgc
     2041 atgcgcgcga gcgtgaggtg cgggctgtag ggacgagcct cgcgtggaaa tccttcggtg
     2101 gagcattcgt cttcgatgtg gcgttgaagt gaggcgaggc cgcccgactc atccacgacg
     2161 ccgagccaga gcacacgcgc cggccccttc ggcggaaacg cgcccgcccc ggagagcttg
     2221 atggggaaag tgctgatgcg cgccgccgcc ctctcggctg cgcctgtaag ctggtcaacg
     2281 cgcgccggtt tgatgtcccc ataaaatttc agcgtcagat gaagtttctc gggccgctcc
     2341 caactcgcgc ggacgtttgg aaacatctcg cgcagtttcg cgatgtaatc gccgacaagc
     2401 tcccgcacct ccgcgctcag ctcgaccgcg cagaagaccc gcaggttttt gtcagacgcc
     2461 gaagtcatcg caagccggcg acgttaaccc ctcccatcgc tcaagggcgc gcgggagtga
     2521 actgtcgcgt ctctctcgcc gcggcgctga ccgcgcgcgg tgtgaagggg aaggcgcggg
     2581 gcgtaacgtt ttgccagtcc gcgagctggt ctttgaagtg agggctggag gggaggccgg
     2641 actggccgag cgtgacggcg tgttgcgtcc tgtcccagtc ggcggtgtcg gcgatgagcc
     2701 gcatcgagac ggccgcgccg acgttgacgg acgccatgcc gccggggttg ccacgctgcg
     2761 gcaacggttg gacggcgaac tgctgtccga cgaaggggac gcccgcgagt ggatggggaa
     2821 agcgcatctg agaatcgcgc ccccacgtcc aagcggcctc gtcggccccg agtcggccgg
     2881 agagggacgc gcgggcgctg cggtgaattt cgtcgatcaa ttccgggtac gtcttgaagc
     2941 tcgttgggag ccagccttgc gggcgcgtgg cgatgaggcg gtcgatgagg gtcgcgctgt
     3001 tggaccagcg aaactccttc gcccgctcgg cgccgaccgc gccctccaca atcttacggg
     3061 cgaactgtgc gcgcatctcg tagacgagca gagcggcgcg cgagtcgggt tcgagacggc
     3121 cgtcccagtc agcgagcagt tgaacggttg agcgccactt ctcgtcgcgg cggtcgagag
     3181 ctgcgccgcg cgcgatgcgg acgacctcgc gcgcgaagtt gtcgccggag atcgagaagg
     3241 tatcgccctg cacggcgcgg aagtcttcga ccgtgagttt ctttttggca gtgagcattt
     3301 gttggatgcg gcgcgcgcgc gtgggcggag cccaaacgtg cgacaggtga tgcgggtagc
     3361 tcgtgccaac gatgcggctg ttggccgtga cgatgatgcc cgaaggcggg tcgtagacgt
     3421 gcggcagctc gttgaagggg atgacgcccg tccactcgcc ttcgtcggtc gagccgtcgt
     3481 aagggacgct gccgtcgccg gtcttacgca gggggatgcg ccccgcgccg tagtagccga
     3541 tgtgaccgtc cacgtccgcg tagacgaaat tctgcaccgg cccctcgtac ttcgacagcg
     3601 ccgcgcggaa gtcgttccag ttgcgcgcgc ggttgaggag acggaagccc tcgaactcgt
     3661 tggagcgcgg gtcgagcgcc gtccagcgca gagagtatcg cgcgccgtcg cgctcaaaga
     3721 tgacggggcc gtggcgcgtg acggtgactt cgtgtgtgac cgtctcggtg gacgcgtcgg
     3781 ccggactctt cctgaccttg atttcctcgc ggcgtacttc ggcctcgctc cagccggcgg
     3841 gcgtcaggta gcgccgcggg ttcgcggggt cgaacttttc gagatagagg tcttgcacgt
     3901 cggggccgag gttggtgaga ccccaggcga tgcgctcgtt gtggccgatg atgacgccgg
     3961 gggcgccggc cgccgtgacg ccggccaccc gcagccccgg cgcgctcaga tgcaccatgt
     4021 accagatggg cggagccgac ggggcgaggt gcgggtcgtt ggcgagcagg ggcttgcccg
     4081 tcgcggtatg ccggccgctg atcacccagt tgttgctcgc cgcgaggtct tcggcgaaga
     4141 gaccggcgcg ctcctgcgtg cgccgaaaaa tcgaatcctc aacggcgagc gcgcgcagcc
     4201 acgcgtcttt gtcaatcgaa tcgacaggca cggcggagcg agtacgcccg gcccccgtgc
     4261 gccgcggcgc ggccttcgca tcagtgccga cgacgagcac gtcgagcggc gaggcgacgg
     4321 gcaggagttc cctgagcttc tcgggcggga ggtctgcgag catggcgcgc atcaggtcga
     4381 tgcgccaggt ggtcgagagg gcctcggcga agtttttgcc gacaacgttg gagtcggccg
     4441 gcgtccagtg gcgtgggcgg tatccgagga tttgaaactc gggcgggagc gacttggcgt
     4501 cgagcgagtc gatgtaggcg ttgacgccgc gcgcgtaggc ttcgacggcg gcgcgggcct
     4561 gcggcgttgc ggcgtcggcc gactgttcgg cgacgcgtgc gaagccgagc gtgcgccgcc
     4621 gcttgtcttc ttcgagcgtg gcgcgtccga agatttccga cagctcgccg cgcgccgtcc
     4681 ggcgcagcaa gtccatctgc cagagccggt cggaggccgt cacgtagccc tgcacgaaga
     4741 agaggtcttc gtcgttctcc gcctcgatgt aggggatgcc gcgctcgtcc cggcgcacgg
     4801 tgacggggcg cttgagctgc gcgagtctga ccgtctggct ctgcgcttgc gcgttggctc
     4861 gcggacgcaa gctgtccgag ggcagaagcg tgaatatgga gagtgtgaag aggagggcca
     4921 gagtggcgcg tgcgccgcgt gaaaatcttt tcatcgaaac ctcgtcggga aggtgttggg
     4981 taaacggaga gaaataataa gggagcggtc ggggaagcgc cacttgaaag taataccgga
     5041 ggggaacacg gatatgacgg ataagacgga ttaacactga tccgtttcta tccgtatcat
     5101 ccgtcctatc cgtgttcccc tccgccacct cactttgagc gacccgcttt aaagacgcgg
     5161 gtcgatgacg atcagacgcg gctccgtcat ctcctccatc gcatagcgga cgccctcgcg
     5221 ccccgcgccg gacatcttga cgccgccgta gggcatgtta tccacgcgga agttcggcgc
     5281 gtcgttaacc aacacgccgc cgaactccag ctcgcggaag gcgcgcaggg cgcgggccat
     5341 gtcgcgtgtg aagacgccgg cctggagtcc gtagcgcgtg ttgttggcct cgtccagagc
     5401 ctcgccgaag tccgcaaacg tctggacggt ggccaccggc gcgaagacct cctcggaaca
     5461 gacgcgcatc tccgcgtgaa cgtccgcgag caccgtcgcc tcaagcagcg aaccgtcacg
     5521 ccggccgccg cacagaagac gcgcgcccgc gccgactgcc tcgttaatcc acccctccac
     5581 gcgccgcgcg gccgcctcgt cgatcatgtt ggaaatctcc gtcgcctcgt cgagcgggtc
     5641 gccggcgcgc atcgcgcgag cgcgcccgac gaacagctcg ttgaaacgct cggcgacttg
     5701 ttcgtgaacg aacacgcgct gggcggagat gcagacctga cccgcgtagg cgaaggcggc
     5761 ggcggccgtg cgctcggcgg cgaactccac gtcggccgtc tcgtccacga tgacgggcgc
     5821 gttgccgccg agttcgagcg ccaccgcctt gcgccccgcg tgctcgcgca gtcgccagcc
     5881 gacctcgacg ctgcccgtaa acgagatcat cgcgactcgc tcgtctttga gtgccacgtc
     5941 gatgtcgggc acatccacgg ggacgacttg cagcgcggct gcggggaggc cggattcgag
     6001 gaagacttcg ccgaggagga gtgcggtcag gggcgtgcgc gggctgggct tgacgatgat
     6061 cgcgttgcgc gaggccagcg ccggggcgac cttgtgcgcg acgagattga gcgggaagtt
     6121 gaagggcgtg atgccgtaga tgacgccgcg gggcacgcgc tcagtccatc cggtgcggcc
     6181 cacgccgccg ggctggccgt cgagcgcaac ggtctcaccc ttgaagcggc gcgcttcctc
     6241 ggaggcgtag gtgaaggtgg cgacggcgcg ctcggtctcg gcacgcgcgg ccttaatcgg
     6301 cttgccggct tcgagcgcga tggtgcgggc gaactcctcg cgccgctcgc ggaggcggtc
     6361 ggcgatgtgt cgcagcgact cggcgaccac gtggcgcgcg agcgcgcgca tctcgcgggc
     6421 cgcaaccgtg gccgatgaaa ttgcctcctc caattcttcg gcggaggcga cggagaagtt
     6481 cgcgaccaac tcgccgttgt aaggcgagcg cacttcctgc gtgcgcgaag atgtacgcca
     6541 cttgccgccg atgagaaaag gcttcgcttc cattgtcggc taataccctt ccgctttctt
     6601 cgtgttgagt tgaatgttag cacaggcggg aggcggggaa tcagacgccc ttttcaaacg
     6661 agccgcggag cggcgaaaga ttgtagccca cggcgtcagc cgtggggaca cgttggaaaa
     6721 gaaagaaagc cccggagggg cgaaagaact tcgacgttgg cgtaggacgc gcgtcaatgc
     6781 tcttccgccc ctccggggct ttctcgtttt tctaaatcgc tgacccacgg cttgcgccgt
     6841 gggctacaat ctttcgccgc ctccggcggc tcgttattga cggctcgacg cggcccccga
     6901 agtagccgcg aggcgtcggc cctcggtgtc cgaccagtcg cagagagtct tcacgtccgc
     6961 gggggtgagc cgcgcttccg ggtggaggat cagataagac ttgagcggca tcgtctcccg
     7021 tttcacctcg cggcagacca tctccaatat ctcgctgcta tcatgtgggg tgtagtcgcc
     7081 ccagtcggaa aagttcagtt cactgcgccc gtcgttgacg tgctcggcca cgaaccagga
     7141 gacgggcgag acgtggctgt accacggcca gttcgtctcg ttggagtggc agtccttgca
     7201 ggcgcgtgtg agcacctgat tgacctccgg cggaacgatg acgtgctgct caattggctg
     7261 ggccgatgcg cgcgccgggg tctccctctc cggccggatg aactggatac cgcacaagac
     7321 gagggccaca gcgaccgcca gcattcgtag aactttcctc agcaccccaa ctccttcctc
     7381 tcctgccaac gggcgacg
//
LOCUS       DASVES010000136        42658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_804618, whole genome shotgun sequence.
ACCESSION   DASVES010000136 DASVES010000000
VERSION     DASVES010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 42658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42658
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804618"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VF240_04915"
     CDS             <1..750
                     /locus_tag="VF240_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX7174606.1"
                     /translation="LGLKPDASEQELKNAYRDLAKVWHPDRFAHDPRLQQKAQEKLKE
                     INEAYEAINSPASGRRHARRPTPPPPSSSTSTSAPRPPRGGPADFTPADFTTVVARRS
                     DAGAGASRLTPVALAVAAVVITFFVSYRFLNSNTAAPAGVSSAAEAELSEEQRSEVVS
                     DAGERVERKPAGKQPRVGEGAGTAAGGEGDSDAGARQSAPTYKTLPTVNRTIDPATGL
                     LATRACPLKAIRTFASGEEPQKFCDADHTRR"
     gene            879..1640
                     /locus_tag="VF240_04920"
     CDS             879..1640
                     /locus_tag="VF240_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174607.1"
                     /translation="MSLEFAVEYPCELRARYEEKHLRAWGRLSSLHARLTTGEHSAPT
                     AEKLRFVERAGDELERMVGEIGVCASCPARLPSEAAGEGELVGCQGRITYPIEAQFEK
                     FLADRAQLALDTIDEQDLPRLMRVLIDRDSPFDGEGTKSLRAVTTPDGLRFFELRLPI
                     RLARQASHLTTDHLFDALAGFTSPDSGQTTYTRELPLAATPDYYDFLDLVLRNDISSS
                     ERERVRARSRNYQQFLRLLAALELAEALNARVLMD"
     gene            complement(1926..2663)
                     /locus_tag="VF240_04925"
     CDS             complement(1926..2663)
                     /locus_tag="VF240_04925"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX7174608.1"
                     /translation="MGDSGSQQNFDEDVETAILDAELFIKYKAPQKALERLQQAVGRN
                     PRSTRLREKLREVALACRQPDEAARQCLALASLYIAREDFESAHDRLLQSKQIDPRIS
                     ITTGLEAIRRARRPDLQPSAPPAEVADSTQTAKPPRPATLAGDLSAISIFDLVQVVEN
                     ARLTGALIIESAGASGRVLFNDGQIVGAEADGETEAPAAFRRLLEITTGAFDFERTAQ
                     SFPVQLNASSNTSLILDSLREIDEGKQ"
     gene            3128..3814
                     /locus_tag="VF240_04930"
     CDS             3128..3814
                     /locus_tag="VF240_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7174609.1"
                     /translation="MHEELLLGTRGAATIRLVDASWANYLQRACASLKAIGARRHGDE
                     GIHSLVMRLKHTRPSTYFHCIRVARVARTTGRALGFDDRQLRQLGHTSMLHDIGKILV
                     PESILSRPRRPTRMERFVLNLHPSFGALLASYFNLSAELRVRTEHHHERWDGRGYPHR
                     LGGEDIPLMARVVQVADSYDAMVSRRSYNAPRSHAEAVAELRAEAGTQFDPRVVEAFL
                     DTYPERSLPR"
     gene            complement(4100..7486)
                     /locus_tag="VF240_04935"
     CDS             complement(4100..7486)
                     /locus_tag="VF240_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7174610.1"
                     /translation="MRHLTSLRTPAPLRVFCYCACLLLLLTTFNLARAQDLDDVTITG
                     RVTDEHGDVLPGAVVTAALAETRSSRTTIADAEGRYRLIELRPGAYTLRAESAGFAAE
                     ERRGVTTIAGQSVRLDFTLRPAALAAETVVVSETDAPAVDTSRTVVGGAITRAEIESL
                     PLHTRSPLDLIFTLPGVTEEPLSTRDAAEDRDPAGRATASSSARTPEEAGVFALSGGP
                     AYSNNLTIDGLDNNDDRAARERFQPSLEAIEEVQVITNQFSAEYGRASGGRVNLRTRS
                     GASRMRGRLFYFFEDEALDANTWNNNRRGLGRLPFQRHTPGFTLGGPVATPQKLFSAR
                     GQDNRARSHFFVAYEFDTTLDSALIDTLVPVEQSAVFPLPAPTAPEGRRREPSATPPN
                     MPAELAPFVESVSTPARNHTLTARLDQHFTDAHNGALLLQLGRLRNLRQFGGGLRLAD
                     TLQGRTRDTESVSYTHNSVFTPALVNQLRAQISSLRPAFEPRGGARGPVVLIEINDPL
                     DDAEAAERSGTLVAGSSTSGATDRRETRLQLQNALTIVRAAHSIKLGADLQRVSSTFI
                     DLADATGTYSFTSAGDFLAAAPSRFRQRFHTESTQKNLYAGLFAQDEWRPLPNLSLTL
                     GLRYEHESIIDDRNNFAPRAAVAYDPLGTGRTVVRLGAGVFFNRALLRTIDDFTLGRQ
                     TVEFDTNDLPTAARRPFIAANLNFPDALAADSAIVRDFGRTLTDFSRRLDPHLKIPES
                     YQANVGFERELGARFVVEANYTFNRGARLWREFNANAPRLPAGFADFADYLLSRDFVN
                     FRNASGLRPVYNAQTAGDLVRFTNRSPDPANPDASARVLEFGVPVTVFNLNSVGSTTA
                     LEAALAALQPLRPDPSRAQVEQLVSAGNSFYHGLTVETRRRHTRHGVTGLGFSFRAAY
                     TLSRLIDDGVVNTSSALRAGDFHGERARSLLDRRHRFVLSGTLDTPRRLGALRLASVL
                     RVASGAPFNVSLGGADRNLDDVGNDRPDFHGDPDLIRWRPPGEPLDARLLEAFQLPTL
                     GRTGNLPRNSGRGPGLFTFDLSITREFRLGEHARLRPVVEINNVLNATVYTFAAEFIN
                     FTGLRETATPEQRRAFIDGFLVPTRTLRQRSVRVGVRFDF"
     gene            complement(7771..8175)
                     /locus_tag="VF240_04940"
     CDS             complement(7771..8175)
                     /locus_tag="VF240_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7174611.1"
                     /translation="MKRSRSKTYTISAVAAQYEIHPQTLRLYEREGLLKPSRSDGNTR
                     LYIDADLERLEVILSLTRDLGVNLAGVEIILNMREKMDAMRSEFVRFFAYLQEHADEF
                     THFTEPPPPEAGALVPSTRAAKLQMRRATKQH"
     gene            complement(8353..9537)
                     /gene="dnaJ"
                     /locus_tag="VF240_04945"
     CDS             complement(8353..9537)
                     /gene="dnaJ"
                     /locus_tag="VF240_04945"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7174612.1"
                     /translation="MAKEDFYKVLGVKRDAKPEEIKKAYRRLARKHHPDVNPGDKSAE
                     ERFKRITEAHDVLSDPKKRTVYDRFGQYSDNLADAAARGATGPGFGAGGGAGRAGAPP
                     FDFTGFDFGTASSSGSSGGGSSFRDIFADLFGGGKEEHEPPRPMPQRGADIEMPLALS
                     FEESVTGMTTNITVNRSEQCSRCNGAGDAGGPLTTCTTCGGAGQVQRAGGRLRFAQEC
                     PDCGGAGKRRTPCAVCHGKGTAPKSEQVKVRIPAGVDTGSRVRVPGKGEGGRLGAPAG
                     DLFIITNVGKHRHFTRKGDNIYVTVPVTVPEAALGAKIEVPTVEGKAQLRIPPGTQSG
                     QKFRLRERGVPSLRNPQARGDQFVEVQVTLPKVVSEETKELLRKFAQMNSENPRTGMG
                     LE"
     gene            complement(9619..11562)
                     /gene="dnaK"
                     /locus_tag="VF240_04950"
     CDS             complement(9619..11562)
                     /gene="dnaK"
                     /locus_tag="VF240_04950"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7174613.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPTVITNSEGGRTTPSVVAFAK
                     DGNRLVGQVAKRQAVTNPENTVYSIKRFMGRRFNEVTEEMKQVPYKVKSAGNDVRVEA
                     GGKEWSPPELSAMILQKLKQAAEDYLGQKVDKAVITVPAYFNDSQRQATKDAGKVAGL
                     EVMRIVNEPTAAALAYGLDKKKEETIAVFDFGGGTFDISILEVGEGVVEVKSTNGDTH
                     LGGDDIDEALIRWIIEEFKRDQGIDLAQDKMSLQRLKEAAEKAKIELSSTMESEINLP
                     FITADASGPKHLVLKLTRARFEQLIDPILKRLMPPVEQALKDAGVDSKKIDEVVLVGG
                     STRIPKVQEMVKGFFGKEPNKSVNPDEVVAIGAAVQAGVLSGEKTDILLLDVTPLSLG
                     IETLGGVSTKLIERNTTIPTRKSEIFSTASDNQTSVEVHVLQGERPMAGDNRTLGKFH
                     LIGIPPAPRGVPQVEVTFDIDANGIVNVQAKDLGTGREQKITITASSGLSKDEIDKMM
                     REAEAHAGEDVKKREEIESKNRLDGLVYQVEKTFSENKEKLDGASVTQLEEAIADSKK
                     ALEEGGVDRMNNAFSALQTASHKLAEALYQNVGQSAGAGDASGEGEQANAAGASGGAG
                     AQGSAGGDDNVIDAEYVDVDDKK"
     gene            complement(11896..12495)
                     /locus_tag="VF240_04955"
     CDS             complement(11896..12495)
                     /locus_tag="VF240_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7174614.1"
                     /translation="MSSRIVTLPNVLTVSRMVMIPLFVTALYYQRFSLALTIFVAAGV
                     SDGIDGFLARRFNQGSQLGQILDPIADKLLLVTSFITLSMPGIIAKTNHLPVPFWVTA
                     AVVGRDVFIVVGAAAINIVTGFRGFRPSLLGKVNTVVQIAAITIILVAARFPALRGYV
                     LPTTYTIVFLFALVSGIHYAFWVSRLMNDERGARGPNQN"
     gene            complement(12579..13430)
                     /locus_tag="VF240_04960"
     CDS             complement(12579..13430)
                     /locus_tag="VF240_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7174615.1"
                     /translation="MATNTSWKRRRVAPTRPTPLRSLTAGLVHLARAAFAEPYALAVE
                     HVSVDLRRLPRPLDGLKIVHLSDIHHSPFTGREQVEHAVEVANSLQPDIIALTGDYIS
                     HEREYIQPCAELLGRLRARRGVYAVLGNHDNWVDAPLITDLFRAEGIQVLNNEGMRFE
                     DAGASFWLAGVNDTMVGLEDLPLALAGSSADEMKLLLAHNPVILRRAARARVDLVLAG
                     HTHGGQVTWRNERDASGRVRRRILRGLGRRGETQIYVTRGVGTVVLPVRYGCRPEVSL
                     LQLRCAE"
     gene            complement(13659..15530)
                     /locus_tag="VF240_04965"
     CDS             complement(13659..15530)
                     /locus_tag="VF240_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX7174616.1"
                     /translation="MTPAIAIVLLLLLVALVLFSSERIPIDIVTILLVIALVLTGTLD
                     VKEAFAGFGNDIVITIAGLFILTGGLVKTGVVDVVGRRLHRIAGGNPFRLAALVMFTA
                     AACASVMKNTTTTAMFVPVVLGIAGRARVQPSKLLMPLAFGAILGGTCTLIGTSTNLA
                     VSGALARLGDSPEFIARLQESPQFVARFTDIESTFKPFSMFELTPVGIAIVAAGMLYM
                     LLLGIYLLPSRGGGDDSLAEQYHIREFMSEVLVLPDSPLVGQTLAEANLSDALDLTVV
                     GILRGKQGRIAPHAEERVQSGDLLLVQGRVEDILRVKTEAGIEIRPDFELREDQHLES
                     GGVELFEVMVLRGSDLNGRTLRGLNFRDRFDLTVLAINRHGVALLSKLSSVTLRFGDV
                     LLVQGTRERVERLSTEGNLLLLEDVSARRGRAGKQVWALAAFGLFLALSVVKYFSGVE
                     VPLSVAVLLGALVLLVTRSVHTAEIYDLIDWRLIVLIAGMISFGTAMEKTHADQYLAD
                     LITNNFAAYGSTAVLAGFFLLTVALTQPMSNQAAALVVLPIAVKAAVTLGLNPRTFAI
                     TVTYAASCSFLTPLEPACVLIYTPGRYRFFDFVKVGSILTVAVFAIVMFLVPIIWPL"
     gene            15682..16182
                     /locus_tag="VF240_04970"
     CDS             15682..16182
                     /locus_tag="VF240_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7174617.1"
                     /translation="MGQQHCIIIIEDQEDLAALYETTLTKAGYSVIKAYTGEEGVAAF
                     QDEGADAVLLDMTLPEMNGVQVLQQIRALNPNVPVVVATGESSEETRRQCERLGVQAY
                     LPKPAGHDELLKAIGRALSDERTLTEEYEVVTLRLPTRVVRCLTGIDENLERAVTSLC
                     EEQASR"
     gene            complement(16292..16795)
                     /locus_tag="VF240_04975"
     CDS             complement(16292..16795)
                     /locus_tag="VF240_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HEX7174618.1"
                     /translation="MSRIHYDVPGINQGPHNTCWLACFRMLIMFRLQVGRPVNARAAA
                     LLEPRLIARFEGLNRGLNPAAFEAVAAEFGLSALHIAGLTRARPAGELEDPFLAYGVL
                     QQRGPFTLGGILPSGSGHAIVVCGASDEPGFDIEFIDPRYGVIRHLGFTTLQRGFPPD
                     GGALFVF"
     gene            16968..17513
                     /locus_tag="VF240_04980"
     CDS             16968..17513
                     /locus_tag="VF240_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174619.1"
                     /translation="MNQGSNCGIVQNSTSGAASGRASVAVNQIANTRERGRTLRHLFF
                     MAVFALVISSGFSAMAQVGSTDRDSPLRITTRTVTAEVSRPLGEEQEVFYSIAARRGY
                     MTIDFSALARDGMNIVLGVEGNGVFESIGPLISGGDDRMEGTVRFRVPSRQALLVNVR
                     YSGNARYTINFSGSAIPSPRR"
     gene            complement(17616..18671)
                     /locus_tag="VF240_04985"
     CDS             complement(17616..18671)
                     /locus_tag="VF240_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174620.1"
                     /translation="MIDAPHTRQQLAAPFLPGVALLLAAVAALAACGDAATGARGKPP
                     SVEQVKRQNKSRPAATPEQEARAPYGAPARLATLEDEAVRESSGLAASRRNPGLFWTH
                     NDSGAAPLLHAFDRDGRRRGVWRVAGAASIDWEDMDAGPGPQPGVTYLYVGDIGDNKR
                     RREEIVVYRVAEPEITPADAQTSARAPRETEPAAILRLRYPDGAHDAEALLVHPSSGD
                     LYVVTKEIVGGAGVYKLAAPPSVAPSVNTLKRVGEVRLPVLFGGLVTGGDISPDGRRV
                     VLCDYTSGYEFVLPGGGAATAGFDGVWNQSPLAIDFGERQQGEAVCYGADGESIFATS
                     EKRPVPFIEVRRAALKK"
     gene            complement(18695..19714)
                     /locus_tag="VF240_04990"
     CDS             complement(18695..19714)
                     /locus_tag="VF240_04990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7174621.1"
                     /translation="MLRVEYREGLYLPDLDLWLDADGPRQSCVISHAHSDHIAEHQRI
                     VATPETARIFRHRRGEAEVETLNYGERRDFGRYALTMVPAGHCLGSAMILVEADGERL
                     LYTGDFKLRPNVAAETAQIVPCDTLVMESTFGDPLYRFPPDVATFDRLYAAVDRALSD
                     DRVPVVLAYALGKGQEALELLLRRGYRVTLHGSVWNVSEIYRELGVEFSGPYERYDRE
                     RLRGRVLITPPGCRKQPMITNLERRYVIMLTGWALSKSAPYMYRGVDLVLPLSDHADF
                     DELVRLVRETGAARVITMHGAKKFAAILRELGFNAEHLAEHPGAGKNTKVKKASTVVE
                     QTLFD"
     gene            19908..20675
                     /locus_tag="VF240_04995"
     CDS             19908..20675
                     /locus_tag="VF240_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7174622.1"
                     /translation="MSQEEKAETGGAHAGGEKPPVDVAYGETLPKEAPVRRRLAARRL
                     LRAEVYYAAALAAFAALTLFVYRNPQIDWDVRAAAAWQGVPGLFEFMRLASVPGDRWI
                     PHAVTTVTVIAFLLLRRFSEASALTLSAGGGALLSRSFKILIARPRPTPELVEVFRPI
                     DTMSFPSGHVTFYVCYFGFLFFVAYAILPRGSTRRRLALALTALPVLLVGPSRVYLGE
                     HWPSDTLGAYLMSGVWVALCLDLYRRWKKPKKVMSDE"
     gene            complement(20747..21199)
                     /locus_tag="VF240_05000"
                     /pseudo
     CDS             complement(20747..21199)
                     /locus_tag="VF240_05000"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(21391..21891)
                     /locus_tag="VF240_05005"
     CDS             complement(21391..21891)
                     /locus_tag="VF240_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174623.1"
                     /translation="MTPCFVCAGTGGSPELSATTAVAAARPAPQPKWRARLPWVVAYT
                     SSESGQWQVYVRSFPDAGGKWMVSTEGGAQPRWRGDGEELYYVAPGRSLMAVEVKWDS
                     ATFEAGVPRPLFEMRNVGGFPGGGGYDVTRDGKRFLVNTAAQEENPRPVTVVLNWTAG
                     LKRGQD"
     gene            22416..23222
                     /locus_tag="VF240_05010"
     CDS             22416..23222
                     /locus_tag="VF240_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174624.1"
                     /translation="MRNRITVVISLLLCCAAGGGAIWANSGPAARGRSWQDERGGGQT
                     SNPLVSVKEKARAAKSNDEAAIRALADEIFSMFNFDQAPAGMDDAIKERLVRAEIKYR
                     QGRAKGSQEFRVAHMVNLLAHKLGAPDYARTNVLEVRRLRMSLLPYTSGLRAQSPADE
                     TRKGEKRSASMSPLEAFVVAVSLLQQKRFNVEYQLTNDEWVALHGGKRSAGSGAKFLE
                     EMKARRRDSRRTDEIDEAIGRGFAAMSPSQILGLPEELLNTLGVERQEGR"
     gene            23228..24013
                     /locus_tag="VF240_05015"
     CDS             23228..24013
                     /locus_tag="VF240_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174625.1"
                     /translation="MKRAKTYLLCATLLCATVFAGCGGGGSPQPGFKAQGEKYVSIAG
                     GGFMFVSATSIRGNWQFDNGSAVGNTTSFGPILSFGPFPVDGGRVPASWNIVAGIPQA
                     ECIGYLEPTQRNVTSGSTQKSRCVTFGIIIPFASAPASVDLQAPPPTMDMTGSDLNTA
                     YGMPYIEYVDQFSGNVLGSATATAVSADGTWLQAPVPDLSSVYSGTYNILISNYLADG
                     SREYVGTSTIDCYGRDIVYDPPPDPDPCGCWPDQPCMPCEIQY"
     gene            24228..24875
                     /locus_tag="VF240_05020"
     CDS             24228..24875
                     /locus_tag="VF240_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174626.1"
                     /translation="MSRKQLFGVLAAVLMSCFDGRGAHAQSDTPRLEVGAQFSTLEID
                     DARGLGERREAGFGGRLTYNLGRHFALEAEINYFPRDYRLVTTDFTGGRVIQGLFGPK
                     AGIRGEKFGLFGKVRPGFQSSGRAARARFPNGNGPDPGDPFGFEFFRATQLTLDVGGV
                     FEYYPSRRYVVRFDLGDTITRYPNVEFVLLPAGTPVRETVYSHKVQFSAGFGFRF"
     gene            24912..25637
                     /locus_tag="VF240_05025"
     CDS             24912..25637
                     /locus_tag="VF240_05025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7174627.1"
                     /translation="MRLKTRRLLASALAAALTCAAGAHDGCLRPALAQKRDGRGTSHG
                     VVYLALGDSTGVGVGAERGGYVERLFARVERARPGSRLINLCARAAATTDVLREQVGR
                     VPAARPTLITVGVGANDLIRGVPAERFAHNYEEIIARLKEENDAPILVMNIPDLALLP
                     AVPAYMRDDARRHILLFNERIGEVAKRYELPVVDLYSRSREFSSHPELFSRDGVHPSD
                     AGYEFWAESVWPAMKKMLHEMPK"
     gene            complement(25748..27436)
                     /locus_tag="VF240_05030"
     CDS             complement(25748..27436)
                     /locus_tag="VF240_05030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7174628.1"
                     /translation="MAKFVGIASERQDALALDVRAGGLRLVAAMERYAVVLLVLLAVG
                     GFYLRAAGLGRVGFAEDEINKLDAVRAYARGDITPNAEHPMVMKTLIFVSMQAAGAWN
                     ARAARGQQISEEAALRLPNVVFGALTVFPLFLLAAFFFGRQTGLLAAAMWAGGVTAVT
                     FNRIAKEDTLLVFFMLWAFYFYIRAKATSGREPRMKRRHYVASAVSFALMLASKYFPH
                     YFGLNMLYHHRARLRQREPGEPSGRTPAVFYWVLLAAFVVFNPALFMPQTWQYLAAYS
                     SEGLQTHSGYLMGETLYRNVMSATPFWGTPVYFYPLFLLNKVPIPVTVALVAGLVRCA
                     RRWREPGPMFLLLMFVLWIVPYSLVGAKWLRYTLSLMPFVYMIAAVGVVAMVRRAAEW
                     LSRTRAPRGNSAWWLVGALAITVFVALPAWAAWNSKPHYALYTNALAPARAGYFFPHD
                     EFYDDGLREAIRYVAEHAPRGATLAHETPGVVRHYLERFGRADLDSRVISDPAFDARD
                     AAAPAYVIVQRGRIYFENREELDALRSSPRFERVYDGVIRGARAVEVYARVGGS"
     gene            complement(27528..28685)
                     /locus_tag="VF240_05035"
     CDS             complement(27528..28685)
                     /locus_tag="VF240_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7174629.1"
                     /translation="MAYLPEDVEIRTTRTEEGAGASGARGVMSQEIKGSDRARRLAPA
                     GLIFAAAGLLLFGYVVWQAGVEKIWAELQKLGVGFAAILALSGLRFVVRSYAWTLCFE
                     TPHRLRLRHALRAFMTGDAIGNVIPLGLVVSEPTKVLLVRERVPVVAAMSAITVENIF
                     YSLSVVLFVFGGAAAMLLSFPLTRALRWTSVGIIVGAAVVFTVGFYVVRTEWRILSGL
                     AERLASRGVGRRALGSRRETLRALEDRVYGFYGRNRRMFLPLLLLEASFHALGVAEAY
                     VTLWFISAEPPTVLAAFVLESVNRVINVVFKFVPLRVGIDEAGTGLFTKVLRFGVSTG
                     VTLAIVRKARMLVWTAVGLAFLVGRGLSVRGVVRDAEQTLAETRLRMKAEG"
     gene            28914..30089
                     /locus_tag="VF240_05040"
     CDS             28914..30089
                     /locus_tag="VF240_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7174630.1"
                     /translation="MSRAAQEMLSGSPLTVYSEDEEMFRASVREFAEGEIRPRVEEMD
                     EHAKMAPEIIKQLFGLGLMSIETPEEYGGTGATFFTAIIGVEELSRVDPSVGVLMDVQ
                     NTLVNNALIRWGSPEQKKKYLPQLAAEKLGAYCLSEAGSGSDAFALATRAVDEGDHFR
                     LTGQKLWITNGNEAEIFIVFANVRPEEGYRGISAFVVEKGFDGISVGKKENKLGIRAS
                     STTEIILDNCRVPKENVLGELGKGYKVSIETLNEGRIGIGAQMIGLARGALEHALRYT
                     GERAQFGKQINQFQGVQFQLAEMATELEAARLMVYNAARLKDAGLPFVKEAAMAKLYS
                     SRAAEHIASKAIELYGGYGFVKDYPVEKLWRDSKIGAIYEGTSNMQLQTIAKLLIGK"
     gene            30187..30504
                     /locus_tag="VF240_05045"
     CDS             30187..30504
                     /locus_tag="VF240_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174631.1"
                     /translation="MATSRIDIFRMMLQSDPDNTVVWFGLAKECEKEGRHDEVVEALT
                     EYLGRADDEGNAYGMLARAYTSLGNREAARRSYQRGAEVALAHGHPSMAEEYRATLET
                     EFD"
     gene            30649..32769
                     /locus_tag="VF240_05050"
     CDS             30649..32769
                     /locus_tag="VF240_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEX7174632.1"
                     /translation="MATELKAREYRGLSREEMVGIYRTMYLSRRVDDKEIQLKGQNKI
                     FFQISGAGHEAVLVAAGRAMKPAYDWFYPYYRDRALCLQLGMTPLEQLLSAVGSADDP
                     NSHGRQMPSHWGHKKLNIVSQSSPTGTQFLQAVGCAEASFRAALIDDIKDKVAGFKGD
                     EVVYCSAGDGTTSEGEFWEAMNTACNLKLPVVFLIEDNGYAISVPVEVQTAGGDISKL
                     VSGFPDLLIQKCDGTDPVESLETMRRAVEYCRQRKGPALVHAKVIRPYSHSLSDDEKL
                     YRPDEERTSDAERDPVKRFGALLVDEGLVTQEELQQLKDEVDREVSEATDLALASPQP
                     APETATQFVYSPDVDPTSAEFDTEAGAEVSGNAGTMVDLINRCLHEEMARDPRVLVFG
                     EDVADCSREENLDKVKGKGGVFKVTANLQRKFGGARVFNSPLAEANIVGRAIGMATRG
                     LKPVVEIQFFDYIWPAYHQIRNELALMRWRSGGDWECPIVIRCPVGGYLKGGAVYHSQ
                     SGDTIFTHVPGLRVVYPSNALDANGLLRTAIRCDDPVLFLEHKHLYRQAYNKSQYPGD
                     NFLIPFGKARTVREGTDLSIITYGALVQRSLVAAKQAEQQQGASVEVIDLRTLAPYDW
                     EAVARTVRKTSKVIVAHEDTRAHGFGAEVAARICDELFDYLDAPVRRVAALDTFVAYA
                     PTLEDHILPQVDDVYKAITELYSY"
     gene            33104..34099
                     /locus_tag="VF240_05055"
     CDS             33104..34099
                     /locus_tag="VF240_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174633.1"
                     /translation="MCKRLLNTACAVALVFAAAAAVNAQDTPKPTTTDTQKPTTTTTV
                     TTQTTQAVQNADGTWTVIEYPANKEVVVDLTPGLNMTAGATGRAKVHRMADHTMLHLD
                     LSGVTGVEALNLYAVDPAGRPTLLGPLTIHNGTVSQDFRTPLDKFMLVLSPEASLTTM
                     ADNTPVLFRSAVPQGFAVVPTASRDREDGAVVGEKVSAVSTSGGTSAYSVPMLNVPGM
                     KRGDDSEIKVNLTGALTGSRVNFKIEPRKDGPVSVTARFHELKESPAGQVFVLWAVSP
                     DNKFTKLGQVVNTGRRNEAEIKSETTLTDFGLLITLEAPEATTPAGAVVGTIIRN"
     gene            34403..35557
                     /locus_tag="VF240_05060"
     CDS             34403..35557
                     /locus_tag="VF240_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HEX7174634.1"
                     /translation="MLKRLLPPWLALALVFLCADAAGAQQDALPELVRRVKPSVVSVI
                     TYNSKGEVHVTGSGFFVRPDRVVTNLHVVESAHRVEIRTFDGKGKTYAVAGLLDADEE
                     GDLALLSVPGSAERARALETSAATPEEGEHVFVIGNPLRLEGSVSDGIVSAVREVPSL
                     GRIIQITAPISHGNSGSPVFNMRGQVVGVVTIKVTNGQNINLAISAARLASLQEGAQT
                     LAFSEFGARKRTGAREPDAVSEWWYKNGLNSLWLGNYDSALNYFESAVSKNPERADAW
                     IQVGFCKVKQGKNSDAIRAFERALRLRPDSVEAHNKLGDAYYYAGNFHQAIESYKRAV
                     ELRPDQSEGYYNLGVAYLEVGDRPAAVAQARTLKSLDSELHRKLLNEIER"
     gene            complement(35725..36399)
                     /locus_tag="VF240_05065"
     CDS             complement(35725..36399)
                     /locus_tag="VF240_05065"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX7174635.1"
                     /translation="MYPAGNLFIPAPHGRLEAILKEPRAAATARGVALVLHPHPLHGG
                     TMHNKVVFRAARALGEAGLVTLRVNFRGVGHSTGAHDHARGGEQEDARAALDHLTGAY
                     PGLPVFLAGFSFGARVGLEVGTRDGRVGALIGIGTPIGMDERDYDFSFLRECRKALLL
                     VHGERDEFGAAADVRSLAAQTPPEARTRVEVIPGAGHFFEGQLDEMSRIIREWAEEEL
                     TRIPGP"
     gene            36706..40329
                     /locus_tag="VF240_05070"
     CDS             36706..40329
                     /locus_tag="VF240_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7174636.1"
                     /translation="MTDIADSGRQQAAPQMRARPTPASALRRYGLAAGSVAAATLLMF
                     ALYYVVGLARGSVPFIFYFGAVILSARYAGRGPTLLTILLSALAADYFFIPPFNSFSP
                     NAESVIQVGVFALVSLFIAHVTSRSVRSEAAAQKSGEELATTLRSIGDAVITTDDAGR
                     VTFMNPVAERVTGWTGAEARGRDLMEVFRIVGEGTREPVENPATRVLREGAVVGLANH
                     TVVVARDGRETPIDDSAAPIRDRAGKVVGVVLVFHDVTERRQAEANIRKSEERLRAVA
                     ETAADAIITIDEQSRILYVNPAAGRIFGHAPADILGHNLTMLMPDYLRRAHEAGLGRY
                     VGTGQRHISWEGVELPGLHRDGHEISLEVSFAEFVEDGRRFFTGIARDITERRRAEEA
                     ITKLAAIVESSQDAVIGKTLEGTITSWNAAAERLYGYTSAEAVGRHISMAAPPERRDE
                     IDEILSRIRNGAKVEHLETVRVRKDGTRFDVSVTVSPIRNSAGVVTGASTIARDITER
                     TRAEESVRASEESYRALADAMPQIVWTARPDGYYDYYNRRWFEYTGMTMEQTEGWGWQ
                     PVLHPDDVELCLRTWSNAVVSGHGYEIEYRFRRASDAQYRWHLGRAEPMRDAEGRILK
                     WFGTATDIHDQKQSADRSRFLAEAGAILASSLDYEATLERLARLTVPALADYCLIDIV
                     DEDNVVARVATSHADPSKEKLIERLRAYPPDLSNDTGVPGVLRTGRPSFVADVTEEVL
                     RSVTRDDAHRELLRQMGLKSYMTVPLVARERIVGALTFASTTDRLRYGPEDLPFAGEV
                     ASSAALAIDNARLYRASRDANRSKDEFLATLSHELRTPLTPIIGWTHMMRAGRLPEAE
                     ATHGLEVVEKNARSLTRLINDLLDMSSIMSGKMSIERAPVDLRQALAEAIETVRPLAE
                     QRGVRLELAATDDADAPAIISGDRTRLVQIFWNLLSNAVKFSGAGARVSVGCRADNGE
                     AWVEVADEGEGIAPDFLPHVFERFRQADGTTTRAHGGLGIGLALVKSLTEAHGGQITA
                     ESRGAGRGSRFVVRLPLLRAPDADDEAPTEGAAGARATASPQPSVPKARVLVIEDSRD
                     TLEMLRVVLSTRGYEVVACESAAEALRLAPAMCFDVIVSDIGLPRVDGYELIGRLRQL
                     AHLSATPAVALTGYASTKDAQAALGAGFDMHVAKPVDPATLVGAVERLLKTRPATVSN
                     SES"
     gene            40483..40992
                     /locus_tag="VF240_05075"
     CDS             40483..40992
                     /locus_tag="VF240_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7174637.1"
                     /translation="MEGLTPTATSQDFFTHQAGSPNPYMSGAPADPAAIPRPAGDDHQ
                     TAKAKPRALVVDDAPDVTEMLAMLLRYAGYDVVTVFCAPDAYETALSEQFDVVVSDIG
                     MPGMNGYELAERLRSLPDYEAVPLIAVTGFAMYDDRERATHAGFDAFLTKPINPSDLI
                     NLVKRLSDG"
     gene            complement(41084..41707)
                     /locus_tag="VF240_05080"
     CDS             complement(41084..41707)
                     /locus_tag="VF240_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174638.1"
                     /translation="MLIEEFGASVASVWQGLRPATRSLVVRSLGATQVAGGGAKVQRT
                     GYDACSEWELSRLLAALDARAADGGDAGLSGEQACALGQVAETCARVLHREARSAEVF
                     AQLLERALRARDYTRVDALADALSGRLAPSETCELARHDNQAVRAIAHEALLQLPVGV
                     LVQLLADPVDAEVARDALEHQADEFESEEARWVVCALDAADSSQDDL"
     gene            41901..42263
                     /locus_tag="VF240_05085"
     CDS             41901..42263
                     /locus_tag="VF240_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7174639.1"
                     /translation="MRPHENLDAWVKAIDFVVTVYKITEPFPKEEKFGLTSQIRRAAV
                     SVPANIAEGAARRSPKEFLHFLSNAQGSASEVETELLIAHRLGYLESTEYLKLRSSLS
                     DIGRMLTGLSQHLKRKDS"
     gene            complement(42352..>42658)
                     /locus_tag="VF240_05090"
     CDS             complement(42352..>42658)
                     /locus_tag="VF240_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7174640.1"
                     /translation="PTRHRGIHLRVLRRDERSGDATVLIRMEPGCSYPAHRHRGLEEV
                     LILQGGYTDDRGPHRAGDYVLNEPGSAHHPTALEDTGEDCIMLAITHGGIELVNRES"
BASE COUNT         7491 a        14000 c        14027 g         7140 t
ORIGIN      
        1 ctcggcctca agcccgacgc ctccgagcag gaattgaaaa acgcctaccg cgatctggcg
       61 aaggtctggc accccgaccg cttcgcgcac gacccccgcc tccaacagaa ggcgcaggag
      121 aagctcaaag agatcaacga ggcttacgag gcgatcaact ccccggcttc cggccgccga
      181 cacgcgcgcc gccccacgcc tcctcctccg tcatcgtcaa cgtcaacctc cgccccgcgc
      241 ccgccgcgcg gcggccccgc cgacttcaca cctgccgact tcacaaccgt cgtcgcacgc
      301 cgcagcgacg ctggtgccgg cgcgtcacgc ctgacgccgg tcgcgttagc agtggccgcg
      361 gtcgttatca cattttttgt gtcttaccga tttctgaact cgaacacggc agctcccgcc
      421 ggcgtcagtt cagcggccga ggctgagctg tctgaggagc aacggtcgga agtggtttcg
      481 gacgcgggcg agcgcgttga gaggaagccg gcaggcaagc agcctcgcgt gggcgagggc
      541 gcggggacgg ccgccggcgg cgagggcgac tcagacgcgg gcgcgcgaca atccgccccc
      601 acatacaaaa cactcccgac cgtcaaccgc acgattgacc cggcgacggg cctactcgcc
      661 acgcgcgcct gccccctcaa ggcgatacgg actttcgcga gcggggagga gccgcaaaag
      721 ttctgcgacg cggatcacac gcgcaggtga gcgtcgagtt tcggcagaat cgcccccgtt
      781 agtggttccg gcaatctctg ttattatgta gccgctggac gtggcaggcg cgcatcgtgc
      841 ggcattgtgt tgggtgttta tggacggagg cgagtgagtt gtctttagag ttcgcggtcg
      901 agtacccctg cgagttgcgc gcgcgctacg aggagaagca cctgcgcgcg tgggggcgtc
      961 tgagcagtct gcacgcgcgt ctgacgacgg gcgagcactc ggccccgacg gccgagaagc
     1021 tgcgcttcgt cgagcgcgcg ggcgacgaat tagagcgcat ggtgggcgag atcggcgtct
     1081 gcgcgtcgtg cccggcgcgt ctgccgagcg aggcggcggg cgaaggcgaa ctcgtcggct
     1141 gtcagggccg catcacctac cccatcgagg cgcagttcga gaagtttctg gccgaccgcg
     1201 cgcagctcgc tttagacacg attgacgaac aagacctgcc gcgtctgatg cgcgtgctca
     1261 tcgaccgcga ctcgcccttc gacggcgagg gcaccaagtc tttgcgggcc gtcaccacgc
     1321 ccgacggcct ccgcttcttc gagctgcgcc tccccatccg cctcgcccgc caagcctcgc
     1381 acctgacgac cgaccacctc ttcgacgccc tcgccggctt cacctccccg gactccggcc
     1441 agacgaccta cacccgcgag ctacccctcg ccgccacccc cgactactac gacttcctcg
     1501 acctcgtcct gcgcaacgac atctcctcct cagagcgcga gcgcgtccgc gcgcgcagcc
     1561 gcaactacca gcagttcctc cgcctcctcg ccgccctcga actcgccgaa gccctcaacg
     1621 cgcgcgtgct gatggattga aagagcggtt ggctattagc ttcaaatcat taacaaggca
     1681 ccgcgccggc gtcagccatc aacgagccgc ggagcggcgg cagaatgtag cccacggcgt
     1741 gagccgtggg gacaagcacc cggacaaaga ttgaagcccc ggaggggcga aagaattgcg
     1801 acgttgtgca caaggcccgt cgtgattctt tcgcccctcc ggggctttcc gatttaattg
     1861 acgactgttc cacgggctaa cgcccgtggc tacattctgc cgccgctccg cggctcgttg
     1921 acggctcact gcttcccttc gtctatttcg cgcaagctat cgaggatgag actggtgttg
     1981 cttgaggcgt ttagctggac ggggaagctc tgggcggtgc gttcgaagtc gaaggcgccg
     2041 gtggtgattt cgaggaggcg acggaaggcg gcgggcgctt cggtttcgcc gtcggcctcg
     2101 gcgccgacga tctgcccgtc gttgaaaagc acgcggccgc tcgcgcccgc gctttctatg
     2161 atgagggcgc cggtcagtcg cgcgttctcg acgacctgca cgaggtcgaa gatgctgatg
     2221 gccgagaggt cgccggcgag cgtggcgggg cgcggcggct tcgcagtctg ggtggaatcg
     2281 gcgacctcgg cggggggggc gctcggttgc aggtcggggc ggcgcgcgcg gcggatggct
     2341 tcgagacccg tggtgatgga aatgcgcggg tcgatctgct tcgactggag caggcggtcg
     2401 tgcgccgact cgaagtcttc gcgcgcgatg tagaggctgg cgagcgccag acactgccgc
     2461 gccgcctcgt ccggctgccg gcaggcgagc gccacctcgc gcagcttctc gcgcaaccgc
     2521 gtcgagcgcg ggttgcgccc gaccgcctgc tggagccgct cgagggcctt ctgcggcgcc
     2581 ttgtacttga tgaacaactc ggcgtcgaga atcgccgtct ccacgtcctc gtcgaagttc
     2641 tgctgtgaac ccgagtcgcc caagcgtccc gcctctccga aagcgcactc tcaactttca
     2701 agatcgacgc gagtttagca caacggccgc ccgccgcgac gaacaccaat cgacgcacat
     2761 cagccccgaa ccgagcacga aatcgtgtgc gatgagaagc cgtcaatttg cccaacggcg
     2821 tacacaatgt gcgacttgtc acggaacctc acgcggtctc ttaacccgcg cgtcgcaaac
     2881 tgcaatttcc tccgctcacg agcggtcaaa accatcgttt cgacgcccgc acatctaacg
     2941 gggccgacta cgttgcacgg aagaaacgtg gcatgactac tgcttaatga acgtgtgcaa
     3001 gccccccttg ccagggttcc aacatccgat gttgtgttga catcagaagg cgcgacactt
     3061 gtcggcgtcc gaggtcaccg cgagatgtgg tttgatcgcc ccgatccagc cgcgaggagt
     3121 tttgacgatg catgaggaat tgctgctggg cacgagaggc gctgccacca tcaggctcgt
     3181 tgacgcgagc tgggccaact atctccagcg cgcctgcgcc agcctcaagg ccatcggcgc
     3241 gcgccgccac ggcgacgaag gcatccactc cctcgtcatg cgcctcaagc acacgcgtcc
     3301 ctcgacctac ttccactgca tccgcgtcgc gcgcgtcgcg cgcaccacgg gccgtgcgct
     3361 cggcttcgac gacaggcaac tccgacagct cggccacacc tcgatgctcc acgacatcgg
     3421 caaaatcctc gtccctgaga gcatcctcag ccgcccgcgg cggccgacgc ggatggagcg
     3481 tttcgtgctc aacctgcacc ccagtttcgg cgcgctgctg gcgtcctact tcaacctctc
     3541 ggccgagctg cgcgtccgca ccgagcatca ccacgagcgc tgggacggcc gcggctatcc
     3601 gcaccggctc ggcggcgaag atatccccct gatggcgcgc gtcgtgcagg tcgccgactc
     3661 ctacgatgcg atggtctcgc gccgctccta caacgccccg cgctcgcacg cggaggccgt
     3721 cgccgagctg cgcgccgagg ccggcacaca gttcgacccg cgcgtcgttg aagccttcct
     3781 cgacacctac ccggaaagga gtttaccccg atgattaact ggatgaagcg ccgcatcgaa
     3841 cgctcgcgcg cccgctgtca cagggcggcg cgcgcgcttc ggcgtccaca ttatgctggc
     3901 atacgcgata aagtgagcct caaagctgag tctgacaact gtgttgggtt cagcgcgaag
     3961 gcgcagagcg caaagagagt gcatcacagg gatgatgcat ttgcgcgagc ttgaatgaac
     4021 actctaactg tccacttagt ttttcgcacc ctccccttct ctgcgattag ttaaccctcc
     4081 ccgtgctcaa gccacgagcc taaaaatcaa aacgcacccc cacccgcacg ctccgctgtc
     4141 gcagcgtccg cgtcggcacg aggaagccgt cgatgaatgc ccggcgctgc tccggcgtgg
     4201 ccgtctcccg cagccccgtg aagttgatga actcggccgc gaaggtgtag acggtcgcgt
     4261 tgagcacgtt gttgatctcg acgacggggc ggaggcgcgc gtgctcgccg aggcggaact
     4321 cgcgcgtgat gctgaggtcg aaggtgaaga ggcccgggcc gcgcccggag ttgcgaggaa
     4381 ggttcccagt gcggccgagg gtcgggagtt ggaaagcttc aaggaggcgc gcgtccaatg
     4441 gttcgccggg aggtcgccag cggatgaggt cggggtcgcc gtggaagtcg gggcggtcgt
     4501 tgccgacatc gtcgaggttg cgatccgcgc cgccgagcga gacgttgaag ggcgcgccgg
     4561 aggcgactct cagcacggag gcgagacgca aggccccgag ccggcgcggc gtgtcgagcg
     4621 tgccggagag gacgaagcgg tgccggcggt cgaggagact gcgggcgcgc tcgccgtgga
     4681 agtcgccggc gcgcagggcg ctcgaagtgt tgacgacgcc gtcgtcgatg aggcgcgaga
     4741 gcgtgtaggc ggcgcggaag gagaagccga gaccggtgac gccgtggcgc gtgtggcggc
     4801 ggcgcgtctc gacggtgagg ccgtggtaga agctgttgcc ggccgagacg agctgctcga
     4861 cctgagcgcg cgatgggtcg ggacgtaagg gttggagcgc ggcgagcgcc gcttcgagcg
     4921 cggtcgtgga gccgacgctg ttcaaattaa agaccgtgac ggggacgccg aactccaaga
     4981 cgcgcgcgga ggcgtcgggg ttcgcggggt cgggcgagcg gttggtgaag cggacgaggt
     5041 cgccggcagt ttgtgcgttg taaacggggc gcaggcccga ggcgttacgg aagttgacga
     5101 agtcgcgcga aagcaggtag tcggcgaagt ctgcgaagcc cgcgggcagg cgcggggcgt
     5161 ttgcgttgaa ctcgcgccac agacgcgcgc cgcggttgaa ggtgtagttc gcctcgacga
     5221 cgaagcgagc gccaagctcg cgctcgaagc cgacgttggc ctgatagctt tcggggattt
     5281 tcagatgggg gtcgaggcgg cgcgagaaat cggtgagggt gcggccgaag tctcggacga
     5341 tggcggagtc ggcggcgagc gcgtcgggga agttgaggtt ggcggcgatg aaggggcggc
     5401 gcgcggccgt cggcagatcg ttcgtgtcga actcgacggt ctgccggccg agcgtgaaat
     5461 cgtcgatggt gcggagcaga gcgcggttga agaagacgcc cgcgccgagg cggacgaccg
     5521 tccggcccgt gccgagtggg tcgtaggcga cggccgcgcg cggggcgaag ttgttgcggt
     5581 cgtcgatgat gctctcgtgt tcgtagcgga ggccgagggt cagagagaga ttggggaggg
     5641 ggcgccactc gtcctgcgcg aagaggcccg cgtagagatt cttctgcgtc gattcggtat
     5701 ggaagcgttg gcggaagcgg gagggtgcgg cggcgaggaa atcgccggcg ctggtgaagc
     5761 tgtaggtgcc ggtcgcgtcg gcgagatcga tgaaggtgga gctgacgcgc tggaggtctg
     5821 cgccgagctt gatggagtgc gcggcgcgga cgatggtgag cgcgttctgg agttggaggc
     5881 gcgtctcgcg gcggtcggtg gcgccggagg tggaggagcc ggcgacgagg gtgccggagc
     5941 gctccgccgc ttcggcgtcg tcgagcgggt cgttgatctc gatgaggacg acggggccgc
     6001 gtgcgccgcc gcgcggctcg aaggcgggcc gcaggctgga gatttgcgcg cggagttgat
     6061 tgactagtgc cggcgtgaag acggagttgt gcgtgtagga cactgactcg gtgtcgcgcg
     6121 tgcggccctg gagtgtgtcg gcgagtcgga ggccgccgcc gaactggcgg aggttgcgga
     6181 gccggccgag ttggaggagg agtgcgccgt tgtgcgcgtc cgtgaagtgc tggtcgagac
     6241 gcgcggtgag cgtgtggttg cgcgcgggcg tgctcacgct ctcgacgaag ggggcgagtt
     6301 cggccggcat gttcggcggc gtggccgaag gctcgcggcg acggccctcc ggcgcggtcg
     6361 gcgcgggcag agggaacaca gcgctttgct cgacggggac gagcgtgtcg atgagcgccg
     6421 agtcgagcgt ggtgtcgaac tcgtaagcga cgaagaagtg agaccgggcg cggttgtctt
     6481 ggccccgcgc gctgaagagt ttttgcggag tggcgacggg gccgccgagg gtgaagccgg
     6541 gcgtgtgtcg ttgaaagggc aggcgcccca gaccgcggcg gttgttgttc caggtgttgg
     6601 cgtcgagcgc ctcgtcctcg aagaagtaga agaggcggcc gcgcatccgg ctcgcgccgg
     6661 agcgggtgcg gaggttgacg cgcccgccgg aggcgcggcc gtactcggcg gagaactggt
     6721 tcgtgatgac ctgaacttct tcgatggctt cgagcgaggg ctggaagcgt tcgcgggcgg
     6781 cgcggtcgtc gttgttgtcg aggccgtcga tggtgaggtt gttggagtag gcggggccgc
     6841 cggagagtgc gaagacgcca gcctcctcgg gcgtgcgggc gcttgaggag gccgttgcgc
     6901 ggcccgcggg gtcgcggtct tcggccgcgt cgcgcgtcga gagcggctcc tcggtgacgc
     6961 cggggagcgt gaagatgagg tcgagcggcg agcgcgtgtg gagcggaagc gactcgatct
     7021 ccgcgcgcgt gatggcgccg cccacgaccg tgcgcgacgt gtccacggcc ggcgcgtcag
     7081 tctcgctgac gacgaccgtc tcggctgcga gtgcagcggg gcgcagggtg aagtcgagcc
     7141 gcacgctctg gccggcgatg gtcgtgactc cccttctctc ttcggcggcg aagcccgcgc
     7201 tctcggcgcg cagcgtgtac gcgcccggac gaagctcgat gagccggtag cgcccttcgg
     7261 cgtccgcgat tgtcgtgcgg gaggagcgcg tctcggcgag tgccgccgtg acgacggcac
     7321 cgggtagtac gtcgccgtgt tcgtccgtga cgcgccccgt gatcgtcacg tcgtccaaat
     7381 cctgtgcgcg cgcaaggttg aaagttgtca gcagaaggag gaggcaggcg cagtagcaga
     7441 agacgcgcag gggcgcgggc gtgcgaagag aggtgaggtg tctcatcgtg tgtcgctccg
     7501 ggttgtgagg cgtcggcggc ccgcggcggt gggcggctga cggacgcgtg attggtttgt
     7561 gcgaggcttc ccgtcggcgt ttgattacgg ctggtcgctt cgtcccttag cgcggacacg
     7621 tgtgccgaac tcgtttgtac gcgcggtgtg aggcggggcg gaagcgcccg gttttaagcc
     7681 gaccgaggcg cgcactttat accttccgag ggacgaagac aagagggttt tttacgaggc
     7741 ggggggcaag ggaggcgcgc gctgacgggg tcagtgctgc ttggtggcgc ggcgcatctg
     7801 gagcttggcg gcgcgcgtgc tggggacgag cgcgccggcc tcgggaggag gaggctcggt
     7861 gaaatgcgtg aactcgtcgg cgtgctcctg aaggtaggcg aagaagcgga cgaactcgct
     7921 ccgcatggcg tccatcttct cgcgcatgtt gaggatgatc tcgacgccgg cgaggttgac
     7981 gccgaggtcg cgcgtcagag agaggatcac ttcgagtcgt tcgaggtcgg cgtcgatgta
     8041 gagccgcgtg ttgccatcgg agcgagaggg cttgagcagc ccctcgcgct cgtagagccg
     8101 aagggtctgc gggtgtatct catactgcgc ggccaccgcg ctgatcgtgt aggttttgct
     8161 cctgctacgt ttcataagtc gagagccgaa aaagcatccg tgactttacg acttgtcctt
     8221 cccgccccgt ctgcggtgga gcaacaggcc cacggcagcc ggtatcacca aaaagaccaa
     8281 agccagcacc gccagcacga acagcgccac ccacacggtc ggttgcgagt gcaacgcgag
     8341 tggcacgcgc gtctactcca gccccatccc cgtgcgcggg ttttccgagt tcatctgcgc
     8401 gaacttgcgg agcagctcct tcgtctcctc ggagacgacc ttcgggagcg tgacctgcac
     8461 ctcgacgaac tggtcgccgc gcgcctgcgg gttccgaaga ctcggcacgc cgcgctcgcg
     8521 caggcggaac ttctggcccg actgtgtgcc gggagggata cgcaactggg ccttgccctc
     8581 gacggtcggc acctcgatct tcgcgccgag cgcggcctcg ggcacggtga cggggacggt
     8641 gacgtagatg ttgtcgccct tgcgtgtgaa gtggcggtgc ttgccgacgt tcgtgatgat
     8701 gaagaggtcg cccgcgggag cgcccagacg cccgccctcg cccttgccgg gcacgcgcac
     8761 gcgcgagcct gtgtccacgc ccgcagggat gcgtaccttc acctgctcgg atttcggcgc
     8821 cgtgcctttg ccgtggcaga cggcgcaggg cgtgcggcgc ttgcccgcgc cgccgcagtc
     8881 gggacactcc tgcgcgaacc gcagacgccc gcccgcgcgc tgcacctgcc ccgcgccgcc
     8941 gcaggtcgtg caagtcgtca gcgggccgcc ggcgtcgccc gcgccgttgc agcgcgagca
     9001 ctgctcggag cggttgacgg tgatgttcgt cgtcatgcct gtgaccgact cctcgaagga
     9061 gagggcgagc ggcatctcga tgtcggcccc gcgctgcggc atcggccggg gaggctcgtg
     9121 ctcctcttta ccgccgccga agaggtcggc gaagatgtcg cggaacgacg acccgccgcc
     9181 cgaggaaccg gacgaggagg ccgtgccaaa gtcgaagccg gtgaagtcga agggcggcgc
     9241 accagcgcga ccagccccgc cgcccgcgcc gaagcccggc cccgtggcgc cgcgcgcggc
     9301 ggcgtcggcg agattgtcgg agtactggcc gaagcggtcg tagacggtgc gcttcttcgg
     9361 atcggagagc acgtcgtgcg cctcagtgat gcgcttgaaa cgctcctcgg ccgacttgtc
     9421 accggggttc acgtccgggt ggtgtttgcg cgccagacgc cggtaggctt tcttgatctc
     9481 ctccggcttc gcgtcgcgct tgacgcccag aactttgtag aaatcttcct tagccatgaa
     9541 aagtgagcag tgagcagtga gcggtgagca gcagaaaact tatctgctta ctgctcaccg
     9601 ctcactgctt actttctgtt acttcttgtc gtccacgtca acgtactcgg cgtcgatgac
     9661 gttgtcgtcg ccgcccgccg agccttgcgc gcccgcgccg ccggaggcgc cggccgcgtt
     9721 ggcctgctcg ccttcgcccg acgcgtcgcc cgcgcccgcc gactgcccga cgttctgata
     9781 gagggcttcg gcgagtttgt gcgaggccgt ctgcaaggcg gagaaggcgt tgttcatgcg
     9841 gtcaacgccg ccctcttcga gcgccttctt cgagtcggcg atggcctctt cgagctgcgt
     9901 gacgctcgcg ccgtcgagct tctccttgtt ctcgctgaag gtcttctcga cctgatagac
     9961 gaggccgtcg aggcggttct tcgactcgat ctcctcgcgc ttcttcacgt cctcgccggc
    10021 gtgcgcctcg gcctcgcgca tcatcttgtc gatctcgtcc ttcgacagac ccgacgaagc
    10081 cgtgatcgta atcttctgct cgcggcccgt ccccaggtct ttggcctgaa cgttgacgat
    10141 gccgttggcg tcgatgtcga aggtgacttc gacctgcggc acgccgcgcg gcgcgggcgg
    10201 gatgccgatg aggtggaact tgcccaacgt gcggttgtcg ccggccatcg ggcgctcgcc
    10261 ctgtaggaca tgtacctcca cgctcgtctg gttgtcggac gccgtggaga agatttccga
    10321 cttgcgggtc gggatcgtcg tgttgcgctc gatgagcttg gtggagacgc cgcccaaggt
    10381 ttcaatgccg agggagaggg gcgtcacgtc gagcaggagg atgtccgtct tctcgcccga
    10441 gagcacgccg gcctgcacgg ccgcgccgat ggcgacgacc tcgtcagggt tcacagactt
    10501 gttcggctcc ttgccgaaga aacccttgac catctcctgc accttcggga tgcgcgtgga
    10561 gccgccgact aagacgacct cgtcgatctt cttcgagtcc acgcccgcgt ccttgagggc
    10621 ctgctcgacg ggcggcatca gacgcttgag aatcgggtcg atgagctgct cgaagcgggc
    10681 gcgcgtgagc ttcaagacaa ggtgcttcgg ccccgaggcg tcggccgtga tgaaggggag
    10741 gttgatctcc gactccatcg tcgaggacaa ctcgatcttg gccttctcgg cggcctcctt
    10801 gagacgctgg agcgacatct tgtcctgcgc gaggtcgatc ccctggtcgc gcttgaactc
    10861 ctcgatgatc cagcggatca aagcctcgtc gatgtcgtcg ccgccgaggt gcgtgtcgcc
    10921 gttcgtggat ttgacttcga cgacgccctc gccaacttcg aggatggaga tgtcgaaggt
    10981 gccgccgccg aagtcgaaca cggcgatggt ctcttccttc ttcttgtcga ggccgtaagc
    11041 gagcgcggcc gccgtcggct cattgacgat acgcatcact tcgaggcccg ccactttgcc
    11101 ggcgtctttc gtcgcctgcc gttgcgagtc gttgaagtag gccggaaccg tgatgaccgc
    11161 cttatcgacc ttctggccga ggtagtcttc cgccgcctgc ttgagcttct ggagaatcat
    11221 cgccgaaagc tcgggcggcg accactcctt gccgcccgcc tcgacgcgca cgtcgttgcc
    11281 ggccgacttg accttgtagg gcacctgctt catctcctcc gtcacctcgt tgaagcggcg
    11341 gcccatgaag cgtttgatcg aatagactgt gttttcgggg ttggtgacgg cctggcgctt
    11401 ggcgacctga ccgacgaggc ggttgccgtc cttggcgaag gcgacgaccg agggggttgt
    11461 gcgcccgccc tccgaattgg taatcaccgt cggctcgccg ccttccatca cggccacgac
    11521 cgaattggtc gtgcccagat cgataccgat aatcttgctc atttctccct gtctccaatc
    11581 agtttagccc ggcttcggcg ggccgaaatc cgtcgcccgt ggccgcacac gcgcccgtct
    11641 actccaaagt tctgacggga atgtgcggcg acttaaatac gccagtaata taatatctga
    11701 gtccgtcgtt gtcaacttta tcttagtgct cggcagcaga cagagtgggg cgggaggaaa
    11761 gatttaagtg cgttcggccg aagttctagc aaatatacgg tttagagtga tattcacttg
    11821 agcatcacgg aagaagattc accgcagagg cgcagaggac gcagaggggg cgcggaggct
    11881 tgagcagagt gcggctcagt tctgatttgg cccacgggcg ccgcgctcgt cgttcatgag
    11941 gcgggagacc cagaaggcgt agtggatgcc ggagacgagg gcgaagagaa agacgatggt
    12001 gtaggtggtc ggcaggacgt aaccgcgcag ggccgggaag cgcgcggcga cgaggatgat
    12061 ggtgatggcg gcgatctgga cgaccgtgtt gaccttcccc agcagagagg gccggaagcc
    12121 ccggaagccg gtgacgatgt tgatggccgc cgcgccgacg acgataaaga cgtcgcggcc
    12181 gacgacggcc gccgtcaccc agaagggcac gggcaggtgg tttgtcttgg cgatgatgcc
    12241 gggcatcgag agggtgatga aggaggtgac gaggaggagc ttatcggcga tggggtcgag
    12301 aatctggccg agctgtgatc cctgattgaa gcgccgcgcg aggaagccgt cgatgccgtc
    12361 gctgacgccg gcggcgacga agatcgtgag cgccagcgag aaccgctgat agtagagggc
    12421 cgtcacgaag agcgggatca tgaccatgcg ggagacggtc aggacgttcg gcagcgtgac
    12481 gatgcgggaa gacattggaa aagtgatgag tgatgagtga taagtgataa gtaagaaagg
    12541 tttttcttat cacttatcac tcatcacttt tcactttatt actccgcaca gcggagttgc
    12601 agcagcgaga cttcgggtcg gcagccgtag cggacgggca ggacgacggt gcccacgccg
    12661 cgcgtaacgt aaatctgcgt ctccccgcgg cgccccaagc cccgcaggat gcgccggcgg
    12721 acgcggcccg aggcgtcgcg ctcgttgcgc caggtgacct gtccgccgtg cgtgtggccg
    12781 gcgaggacta aatcgacgcg ggcgcgagcc gcccggcgca gaatcacggg gttgtgcgcg
    12841 agcagcagtt tcatctcgtc ggcactcgag ccggcgagcg cgagcggcaa atcttcgagt
    12901 ccgaccatcg tgtcgttgac gccggcgagc cagaaggagg cgcccgcgtc ctcgaagcgc
    12961 atcccctcgt tgttgagcac ctgaatgccc tcggcgcgga agaggtcggt gatgagcggg
    13021 gcgtccaccc agttgtcgtg gttgccgagg acggcgtaga cgccgcggcg ggcgcgcagg
    13081 cggcccagaa gctcggcgca gggctgaatg tactcgcgct cgtgcgagat gtaatcgccg
    13141 gtcagggcga tgatgtcggg ctggaggctg ttggcgacct cgacggcgtg ctcgacctgc
    13201 tcgcggcccg taaaggggct gtggtggatg tcggagaggt ggacgatctt caagccgtcg
    13261 agcgggcgcg ggagccggcg caagtccaca ctgacgtgct cgaccgcgag cgcatacggc
    13321 tcggcgaagg cggcgcgggc cagatgcacg aggccggccg tcagggagcg caagggggtg
    13381 ggccgcgtcg gcgcgacacg gcggcgcttc cagctagtgt tggtagccat ccgttttttt
    13441 ccgttttatc gagggaaagg tttgaaagct gctccgatta ccgccctgtt cccacgtcgt
    13501 cggctcgttc ccgaagcccg cgcaatatat caaggcaaaa gtaaaaaggc aaaaggcaaa
    13561 agcgtctgag acgggccgca actaaaatat aacttatccc ccaaacatcc cctgctttct
    13621 taaacttttg ccttttgcct ttttactttt gccttctcct acagcggcca gatgattggg
    13681 acgaggaaca taacgatggc gaagacggct acggtgagga tcgagccgac cttgacgaaa
    13741 tcgaagaagc ggtagcggcc cggcgtgtag atgagcacgc aggccggttc cagcggcgtc
    13801 aaaaacgagc aggaggcggc gtaggtaaca gtgatggcga aggtgcgcgg gttgaggccg
    13861 agcgtgacgg cggccttgac ggcgatgggg aggacgacca gcgccgccgc ctgattcgac
    13921 atcggctgcg tcagcgccac ggtgaggagg aagaagccgg ccaagacggc cgtcgagccg
    13981 taggcggcga agttgttggt gatgaggtcg gcgaggtact ggtcggcgtg cgtcttctcc
    14041 atcgccgtgc cgaaggagat catgccggcg atgaggacga tcagacgcca gtcgatgagg
    14101 tcgtatatct ccgcggtgtg gacgctgcgg gtgactaaaa ggacgagcgc gcccaaaagc
    14161 accgccaccg agagcggcac ctcgacgccg ctgaaatact tcaccaccga gagcgccaga
    14221 aagagaccga aggcggcgag cgcccagacc tgtttgccgg cccgcccgcg ccgcgccgac
    14281 acgtcttcga ggagcagcag attcccttcg gtcgagagcc gctcgacgcg ctcgcgtgtt
    14341 ccctgcacca gcagcacgtc gccgaagcgg agcgtgacgg agctgagctt gctcaatagc
    14401 gcgacgccgt ggcgattgat ggccagcacc gtgaggtcga agcggtcgcg gaagttcaga
    14461 ccccgcaacg tccggccgtt caagtcagac ccgcgcagaa ccatcacctc gaacagctcc
    14521 actccgcccg actccagatg ctgatcctcg cgcagctcga agtcgggccg aatctcgata
    14581 ccggcctccg tcttgacgcg caggatgtcc tcgaccctgc cctgcacgag caaaagatcg
    14641 ccggactgca cccgctcctc ggcgtgcggg gcgatgcgcc cctgcttgcc gcgcaggatg
    14701 ccgaccacgg tgaggtcgag cgcatccgag aggttggcct cggccaaagt ctgcccgacg
    14761 agcggcgaat cggggaggac taacacttcg gacatgaact cgcggatgtg gtactgctcg
    14821 gcgagcgagt cgtcgccgcc gccgcggctg ggcagcaggt agatgccgag caggagcatg
    14881 tagagcatcc cggcggcgac gatggcgatg ccgaccgggg tgagttcaaa catcgagaag
    14941 ggcttgaagg ttgactcgat gtcggtgaag cgcgcgacaa actgcggcga ctcctgaagg
    15001 cgcgcgatga attcggggct gtcgccgagg cgcgcgagcg cccccgagac ggcgaggttc
    15061 gtcgaggtgc cgatgagcgt acacgtcccc ccgaggatgg cgccgaaggc aagcggcatt
    15121 aaaagtttcg acggctggac gcgcgcccgg ccggcgatgc ctaagacgac ggggacgaac
    15181 atcgccgtcg tcgtcgtgtt cttcatcacg gaggcgcagg cggcggccgt gaacatgacg
    15241 agcgcggcga gtcgaaacgg gttcccgccg gcgatgcggt ggaggcggcg gccgaccacg
    15301 tccaccacgc ccgtcttgac gagtccgccg gtgaggatga agaggccggc gatggtgatg
    15361 acgatgtcgt tgccgaagcc ggcgaaggct tccttcacgt cgagcgtgcc cgtgagcacg
    15421 agcgcgatca cgagcaggat ggtgacgatg tcaatcggga tgcgctcgga cgaaaagagg
    15481 acgagcgcga cgaggaggag gaggaggacg atggcgatgg cgggagtcat gggaggcctc
    15541 tgctatactg cgtcctctac cgttcgacaa tttccgattc agggttttcc ggttgtgggg
    15601 aggagtctac aacgaagcgc ggaaatccga aatcgaaaac cgccgaccgg ggccgtatac
    15661 aatcggggga gacacgcgcg catgggccag cagcactgca tcatcatcat cgaggatcag
    15721 gaagacctcg ccgcgctcta cgagacgacg ctcacgaagg ccggctattc cgtcatcaag
    15781 gcctacacgg gcgaggaggg cgtggccgcc tttcaggacg agggcgccga cgccgtcctc
    15841 ttagacatga cgctgcccga gatgaacggc gtccaggttc ttcagcagat acgcgctctc
    15901 aacccgaacg tccccgtcgt cgtcgccacg ggcgagtcga gcgaggagac gcgccggcag
    15961 tgtgagcgcc tcggcgtcca ggcgtacctg ccaaagcccg cgggccacga cgaactcctg
    16021 aaagccatcg gccgcgcgct ctccgacgag cggacgctga cagaggagta cgaggtggtc
    16081 acgctgcgcc tccccacgcg cgtcgtccgc tgcctcaccg gcatcgacga gaatctggag
    16141 cgcgccgtca cctcgctgtg cgaagagcag gccagtcggt agggtttgag ttttgcgccg
    16201 gatggcgata tatagcggct cgcgttcggg tgtgagtcat ccgggccgct tcgtgtgact
    16261 tcgtggttca cacctttcct tgtccccgca ctcagaagac aaacagtgcg ccgccgtcgg
    16321 gtggaaaccc tcgctgcaaa gttgtaaacc ccaagtgccg tatgacccca tatcgaggat
    16381 cgataaactc aatatcgaat cccggctcat ccgaagcacc acaaacaacg atggcatgcc
    16441 ccgatccgct cggcaggatg ccgccaagcg tgaacggccc gcgctgctga agcacgccgt
    16501 aggcgaggaa agggtcttcc aactcgccgg cgggtcgggc tcgcgtcagt cccgcgatgt
    16561 gcagggccga aagaccaaac tcagcggcca cggcctcaaa cgccgccgga ttaagcccgc
    16621 ggttcagccc ttcaaatctg gcgatcaatc gcggttcgag gagcgccgcg gcccgcgcat
    16681 tgaccggccg gcccacctgc aatcgaaaca tgatgagcat gcgaaaacag gcaagccagc
    16741 acgtattgtg ggggccttga ttgataccgg gcacatcgta gtgaatgcga ctcatgtttg
    16801 attctgcctc cggtgttgcg gcgggcgctt gtggctcaga cgtgctgagc caatcccatt
    16861 attcagcaaa ataaattctt tgccgtctat tgatgataca gtgggcggag gaagtaaatc
    16921 cgattttcgc ctcgccttga aaaattaaat tgtttggagg aaacaggatg aatcaaggtt
    16981 caaattgcgg aatcgttcaa aattcgacat cgggtgcggc gagcggccgg gcgtcggtgg
    17041 cggtcaatca aatcgcaaac acacgggaga gaggccgcac tttgcgtcat ctatttttca
    17101 tggccgtttt cgcgttggtg atttcgagcg gcttttccgc gatggcgcaa gttggttcaa
    17161 ccgacagaga cagccccctg cgaattacga cccgaacagt caccgctgaa gtcagccgcc
    17221 cgctcggcga agaacaagag gtattttatt cgattgcggc aagaagagga tacatgacga
    17281 tagatttttc ggcccttgcc cgcgatggaa tgaacatcgt tctcggcgtt gaaggcaacg
    17341 gggtttttga atcaatcggg ccgctcatca gcggcggcga cgataggatg gaagggactg
    17401 ttagattcag agtcccgagc cggcaagcac tgctggtcaa cgtccgttac agcggaaacg
    17461 cgcggtatac gattaatttt agcggctccg ctatacccag cccacgcaga taaaatttta
    17521 gcagcggcct tgctctgttg ccatcctcgg tttctcagtc gcgaacgatt actgctgagt
    17581 aatcgttcgc gactgatcac gcccgcgcgc ccgcttcact tctttaacgc cgcgcgcctg
    17641 acctcgatga aaggcactgg ccgcttctcg ctcgtggcga agatggactc gccgtcggcc
    17701 ccgtagcaga cggcctcccc ctgctggcgc tcgccgaagt cgatggcgag cggcgactga
    17761 ttccacacgc cgtcgaaccc cgccgtagca gccccgccgc ccggcagaac gaactcatag
    17821 ccgctcgtgt agtcgcacag cacgactcgc cgcccgtccg gcgagatgtc gccacccgtg
    17881 acgaggccgc cgaacagcac gggcaggcgc acttcgccga cgcgcttgag agtgttgacg
    17941 gaaggcgcga ccgacggcgg cgcggcgagt ttgtaaacgc cggccccgcc gacgatctct
    18001 ttggtgacga cgtagaggtc gccgctcgac ggatgcacga gcagcgcctc cgcgtcgtgc
    18061 gcgccgtcgg ggtagcgcag ccggaggatt gcggccggct cggtctcgcg cggggcgcgc
    18121 gcgctcgtct gcgcgtcggc cggagtgatt tctggttccg ccacgcggta gacgacgatc
    18181 tcctcgcgtc gccgcttgtt atcgccgatg tcgccgacgt agaggtacgt cacgccgggt
    18241 tgcggccccg gcccggcgtc catgtcttcc cagtcgatgg acgccgcgcc ggcgacgcgc
    18301 cacacgccgc gccggcgacc gtcgcggtcg aaggcgtgga ggagcggcgc cgcgcccgag
    18361 tcgttgtgag tccagaagag accggggttg cggcgcgagg cggcgaggcc gctgctctca
    18421 cgcaccgcct cgtcttcgag cgtcgcgaga cgcgccggcg ctccgtaagg ggcgcgggcc
    18481 tcttgctctg gcgtggcggc gggtcgcgac ttgttctgcc gcttgacctg ttcgacggac
    18541 gggggctttc cccgcgcgcc ggtggcggcg tcgccacagg ccgcgagcgc cgcgaccgcg
    18601 gcgaggagga gcgcaacgcc cggaaggaac ggggcggcga gttgttggcg cgtgtgcggc
    18661 gcgtcgatca taacgttcgc ggtaggcggc cctttcagtc gaacaaagtc tgctcgacga
    18721 cggtcgaagc ctttttgact ttcgtgttct tgcccgcgcc ggggtgctcg gcgaggtgct
    18781 cggcgttgaa gcccaactcg cgcaggatgg cggcgaactt cttcgcgccg tgcatggtga
    18841 tgacgcgcgc ggcgccggtc tcgcgcacca agcggacgag ttcgtcgaag tcggcgtggt
    18901 cggagagcgg cagcacgaga tcgacgcccc tgtacatgta aggcgcgctc ttcgacaagg
    18961 cccagcccgt gagcatgatg acgtagcggc gttcgaggtt cgtaatcatc ggctgcttgc
    19021 ggcagcccgg cggggtgatg agcacgcggc cccgcagacg ctcgcggtcg taacgctcgt
    19081 aaggcccgga gaactccacg cccagctcgc ggtagatctc cgacacgttc cacaccgagc
    19141 cgtgcaacgt cacgcgatag ccgcgccgga gcaaaagctc cagcgcctcc tgccccttcc
    19201 ccagcgcgta ggccaagacg acgggcacgc ggtcgtcaga gagcgcgcgg tcaacggccg
    19261 cgtagagtcg gtcgaaggtg gccacgtcgg gcgggaagcg atagagcggg tcgccgaagg
    19321 tggattccat gacgagcgtg tcgcagggaa cgatctgcgc cgtctcggcc gcgacgttgg
    19381 gccgcagctt gaagtcgccc gtgtagagca gtcgctcgcc gtccgcttcg actaagatca
    19441 tcgccgagcc gagacagtgg ccggcgggca ccatcgtcag cgcgtagcga ccgaagtcgc
    19501 gccgctcgcc gtagttgagt gtctcgacct cggcctcgcc gcggcggtgg cggaagatgc
    19561 gcgccgtctc gggcgtggcg acgatgcgtt ggtgctcggc gatgtgatcg gagtgcgcgt
    19621 gcgagatgac gcacgactgt cgcggcccgt cggcgtcgag ccacaggtcg aggtcgggca
    19681 ggtacagccc ctctctgtat tcgactctca gcaaacccct tgttcccttt ccccgcgtcg
    19741 gccctgctcg acggcctggt cgtcgcgacc cttcgcgcgc gtcgcttcgg tgcttgccgc
    19801 gagcacgcgt gttcgtttac tttcgggaga aattcgctgt aattataaca cgcggccgcg
    19861 cgcgcgggcg ggacgcgccg ggcgtccact tcaggcgtcg aaaaggcatg agtcaagagg
    19921 aaaaagccga aacgggcggc gcgcacgccg gtggtgagaa gccgcccgtg gatgtcgcct
    19981 acggggagac gcttccgaag gaggcgcccg tgcgaaggcg tctggcggcg cgacgcctgc
    20041 tgcgcgcgga ggtttactat gcggcggcgc ttgcggcctt cgccgcgctg acgctgttcg
    20101 tctaccgcaa cccgcagatc gactgggacg tgcgggccgc cgccgcgtgg cagggcgtgc
    20161 cgggcctctt cgagttcatg cgcctcgcct ccgtgcccgg cgaccgttgg attccgcacg
    20221 ccgtgacgac ggtgaccgtc attgccttcc tactgttgcg acgcttcagc gaggcgtcgg
    20281 cgctcacgct ctcggcgggc ggcggcgcgc tcctcagccg ctcgttcaag atactcatcg
    20341 cgcggccccg ccccaccccc gaactcgtcg aggtcttccg ccccatcgac acgatgagct
    20401 tcccgtcggg ccacgtcacc ttctacgtct gctacttcgg cttcctcttc ttcgtcgcct
    20461 acgccatcct gccgcgcggc tcgacgcgac gccgcctcgc gctcgcgctc accgcgctgc
    20521 ccgtcctgct cgtcggcccc tcgcgcgtct acctcggcga acactggccg agcgacaccc
    20581 tcggcgccta cctcatgagc ggcgtctggg tcgccctctg cctcgacctg tatcgtcggt
    20641 ggaagaaacc aaagaaagtg atgagtgatg agtgatgagt gataagagaa agcgtctgtc
    20701 tttcgccttt tcttatcact tatgcactca tcactcatca ccagtctcaa gccttcttct
    20761 tcgtcaggaa gttgcacgcc tggtaagcgc ccgtggggtc gatggcggtg cggccctctt
    20821 cgatgtggcg gatacggccg tctttgtcca cgacgaaggt ggcgcggcgc aggtagcccg
    20881 acttcttgtc gaggatgccg tagtcttcga cgactttgcg gtgccagtcg gagaggagcg
    20941 ggaaggtgac tttgatgtct tcggcgaagc gcttgttggc gaagaagctg tccatgctga
    21001 tgccgaggac ttgggtctcg gtcgcgtcga atttggcgat atcagactga tacgcctgta
    21061 gctccttcgt tcaaccgccg gtgaaggcga ggacgtagaa ggcgagcacg accgacttct
    21121 tgttgcggaa gtcggagagc ttgacctctt tgccctcggt gtcgcggagc gtgaagtcgg
    21181 gggccaagtc gccgaccttg aggtgcgtct gcggcggggc gggcgtctgc gcgggctgcg
    21241 ccgtctgcgc gggttgttgg gccggcttgc cggcgggcgt cggctgcggc tgttgcgcgt
    21301 gcgcgcccgc ggcgagcgcg agcaacagcg ccggccaggt gatttttcta aacatcgatt
    21361 ttcttatcct ccttacggcc tatccttacc ctaatcttgg cctcgcttca agccggccgt
    21421 ccagttgagg acgaccgtga cggggcgggg gttctcttcc tgcgcggccg tgttgacgag
    21481 aaagcgcttg ccgtcgcgcg tcacgtcgta gccgccgccg ccggggaagc cgccgacgtt
    21541 acgcatctcg aagagcggcc ggggcacccc ggcctcgaac gtcgcggagt cccatttcac
    21601 ttccacggcc atcaggctcc tgccgggggc gacgtagtac aactcttcgc cgtcgccccg
    21661 ccagcgcggc tgcgccccgc cctcggtcga gaccatccac ttgccgcccg cgtcggggaa
    21721 gctgcggacg tagacctgcc actgccccga ctcggacgac gtgtaggcga cgacccacgg
    21781 gaggcgtgcg cgccacttcg gctgcggcgc cggccgcgcc gccgcgactg ctgtcgtcgc
    21841 gctcaactcg ggactcccgc ccgtgccggc gcagacgaaa cacggtgtca attccgcctt
    21901 caatcgcggc gaaacttttt ttattgactt ttttattgac aagagcagat tccttgcatt
    21961 agtctctgcc cgtttacata gtccagccgt ttaccgcgct gtccttacgt tttctaacag
    22021 ctgagattcc ccgctcgttg agcctcacaa tagttgattc aagagagtag tcaggtggtg
    22081 tattttcccc acgctctgga gcttccgcta aaccctaatc agcagtcgat taggccggcg
    22141 acgagcgcac agcggggggc atccgtgaca tccgattcgc ggagaaggaa aagcctcgct
    22201 atcactggcc ggtgatagcg aggctttcta acaacacagc ctctgccgca ttcctgcgcc
    22261 aagggtgcat cgctaggcaa cccgcatctt agcgcaagtc gtagcagtgg ctcaaccccg
    22321 aggagccagg gactcgacgt gcgcgccgac tctccacacg gaagcgacct accccgccgc
    22381 gtcatggctc ctcacagcaa taggaggcat gtcaaatgag aaaccgaatt acggtggtca
    22441 tctccttgct cctctgctgc gctgcaggag gcggagcaat atgggccaac tcaggcccgg
    22501 ccgcccgcgg tcgatcctgg caggacgagc gcggcggagg tcaaacttca aatccgctgg
    22561 tgagtgtgaa ggagaaggct cgggcggcca agagcaacga cgaagccgct atccgcgccc
    22621 tggcggacga aatcttcagc atgttcaact ttgaccaggc cccggccggg atggacgacg
    22681 ccatcaagga gcgcctggtg cgcgccgaga tcaaataccg tcagggccgc gctaaagggt
    22741 ctcaggagtt cagggtcgcc cacatggtca acctgctcgc gcacaagctc ggcgcgcccg
    22801 actacgcgcg cacgaacgtt ctcgaggtgc ggagactccg catgagcctt ctgccttaca
    22861 cgtccggtct gcgagcgcag tcgcccgccg acgaaaccag gaagggcgaa aaacgcagcg
    22921 cctcgatgtc tccgctcgaa gcgttcgttg tcgcggtctc gctcttgcag cagaagcggt
    22981 tcaacgtcga gtaccaactg accaacgacg agtgggtcgc gctccacggc gggaagcggt
    23041 ctgccgggtc gggcgcaaag ttcctcgaag agatgaaggc gcgccggcgc gattcgcggc
    23101 gcaccgacga gatcgacgag gcgatagggc ggggcttcgc cgcgatgtcc ccgtcgcaga
    23161 tactcggcct gcctgaagaa ctgctgaaca ctctcggcgt ggaacgccag gaggggaggt
    23221 gaagacaatg aagagagcga aaacttatct actgtgtgcg accctcttgt gcgcgaccgt
    23281 gttcgccggg tgcggcggcg gcggcagccc gcaacccggc ttcaaggcgc agggcgagaa
    23341 atacgtttcg attgccggcg ggggattcat gttcgtctcc gccaccagca tccgcgggaa
    23401 ctggcagttc gacaacggca gcgccgtcgg caataccacg tccttcgggc cgatcttgag
    23461 tttcgggcct ttcccggttg acggcggcag ggttccggcg agctggaaca tcgtcgcggg
    23521 catcccgcag gcggaatgca tcggttacct ggagccgacc cagagaaacg tcacctcggg
    23581 cagcacccag aaaagcaggt gtgtgacgtt cgggattatc atcccattcg cctcagcacc
    23641 cgcgtccgtc gatctgcaag ccccgccccc gaccatggac atgaccggga gtgacctcaa
    23701 caccgcctac gggatgccgt acatcgagta cgttgaccag ttcagcggga acgtcttagg
    23761 cagcgcgacg gcgacggcgg tgagcgccga cgggacgtgg ctgcaagccc cggtgccgga
    23821 tctctcgtcc gtgtactccg gcacctacaa catcctcatc agcaactacc tggccgacgg
    23881 cagccgggag tacgtcggca cttcgacgat tgactgctac gggagggaca tcgtgtacga
    23941 cccgccgccc gaccccgacc cgtgcggctg ctggccggat cagccgtgca tgccgtgcga
    24001 aattcaatac taggtgcgat gcctgagggc ttgacgaagc cgtcaagccc tcaggcatcc
    24061 acagttcagg cgcccgtccg atgtcgggcg cctcaggcag aggggcacgt ccccgagcgt
    24121 ctcccgaacc gatacgctcg ggggcgcggc cgcccgccgc cgcgtgggcg cggcgggtcg
    24181 ccagtcgcgc accggatttc attccaactc aaaggagtcg ctcaccgatg tcccgaaagc
    24241 aacttttcgg cgtcctcgcg gccgtgctga tgtcctgctt cgacggccgt ggcgcgcacg
    24301 cacagtcgga cacgccgcgc cttgaggtgg gggcgcaatt ctccactctc gaaatagacg
    24361 acgcgcgcgg tctgggggag aggagggagg caggcttcgg aggcaggctc acttacaacc
    24421 tcggcaggca cttcgcgctt gaggcggaga tcaattactt ccccagggac taccggctgg
    24481 tgacgacgga ctttaccggc gggcgcgtca ttcaggggct gttcggcccg aaggcaggga
    24541 tacgcgggga gaagttcggc ctcttcggca aggtgcgacc cggttttcaa agctccggcc
    24601 gggccgcccg cgcccggttc cccaatggca acggccccga cccgggcgac ccgttcggct
    24661 ttgaattttt tcgcgccacg caactcacgc tggacgtggg gggagtgttc gaatattacc
    24721 cgtcccgccg ctacgtcgtg cggttcgatc tgggtgacac gatcacgcgc tacccgaacg
    24781 tcgagtttgt ccttctcccg gcaggcactc ccgtcaggga aaccgtctac tcccacaagg
    24841 tacagttcag cgcgggcttc ggcttccgct tctgacaagc ccgaacccgc cgcggacgcg
    24901 gtaaaaaccc catgcgcctc aaaaccagga ggctactggc atccgcactc gccgccgcgc
    24961 tcacctgcgc tgcgggcgca cacgacggct gtctgcgccc cgccctggcg cagaagaggg
    25021 acgggcgcgg cacgtctcac ggcgtcgtgt acctcgcgct cggggattcg acgggggtgg
    25081 gggtgggcgc cgaacgcggc ggctacgtcg agcggctttt cgcccgggtc gaacgcgcgc
    25141 gccccggctc tcggctgata aatctctgcg ccagggcggc ggcgacgacc gacgtcctgc
    25201 gcgaacaggt ggggcgtgtc cccgccgcac gaccgacact gatcacggtc ggcgtcgggg
    25261 ccaacgatct gatccggggc gtcccggcgg agcgattcgc gcacaactac gaagagatca
    25321 tcgcccggct caaggaggaa aatgacgccc ccatactcgt catgaacatc cccgacctcg
    25381 cgctgctgcc cgccgtgccg gcttacatgc gcgacgacgc gcgccggcac atcctcctct
    25441 tcaacgagcg aatcggggag gtcgcgaagc gatacgagct gcccgtggtt gacctctaca
    25501 gccggagccg ggagttctcc tcacacccgg agttattttc ccgagacggg gttcacccgt
    25561 cagacgcagg gtacgagttc tgggcggagt cagtgtggcc ggctatgaaa aagatgcttc
    25621 acgaaatgcc gaaataatca tccacctcgg tctgctctcg ctcccgccca tccgactaac
    25681 gcgcgtcgtc gaggctgaag cggcggcggc ctctcgctgg agacattccg gggcgccggg
    25741 cgcgcgctca actgccgccg acgcgcgcgt agacctcgac ggcgcgcgct cccctaatca
    25801 ccccgtcgta gacccgctcg aacctggggc ttgagcgcag ggcgtccaac tcctctcggt
    25861 tctcgaaata gatgcggccg cgctggacga tgacgtaggc gggcgccgcg gcgtcgcggg
    25921 cgtcgaaggc cgggtcggag atgacgcgcg agtcgaggtc ggcgcggccg aagcgctcga
    25981 ggtagtggcg gacgacgccc ggcgtctcgt gcgcgagggt cgcgccccgc ggcgcgtgct
    26041 cggcgacgta ccggatggcc tcgcgcagtc cgtcgtcgta gaactcgtcg tgcgggaaga
    26101 agtagccggc gcgcgccggg gcgagcgcgt tagtgtagag ggcgtagtgc ggcttggagt
    26161 tccaggccgc ccacgcgggg agcgcgacga agaccgtgat ggcgagcgcg ccgactaacc
    26221 accacgcgga gttgccgcgc ggcgcgcgcg tccggctcag ccactcggcc gcgcgccgca
    26281 ccatcgccac cacgcccacc gccgcgatca tgtagacgaa tggcatcagc gagagcgtgt
    26341 agcgcagcca cttcgcgccg acgagcgaat agggcacgat ccacagcacg aacatcagca
    26401 gcagaaacat cggccccggc tcgcgccacc gccgcgcaca tcgcaccagc ccggcgacga
    26461 gcgccaccgt caccgggata ggcaccttgt tgagcagaaa gagggggtag aaatagacgg
    26521 gcgtgcccca gaacggcgtg gccgacatga cgttgcggta gagcgtctcg cccatcaggt
    26581 agcccgagtg cgtctgaagc ccctcgctcg aataggcggc gaggtattgc cacgtctgcg
    26641 gcatgaagag cgccgggttg aagacgacga aggcggctaa gagcacccag tagaagacgg
    26701 ccggcgtgcg tcccgaaggc tcgcccggct cgcgctgccg gagacgcgcg cggtgatggt
    26761 agagcatgtt cagcccgaag tagtgtggga agtacttcga ggccagcatc agcgcgaagg
    26821 agacggccga tgcgacgtag tggcgtcgct tcattcgtgg ctcgcgcccg ctcgtcgcct
    26881 tcgcgcggat gtagaaatag aaggcccaga gcatgaagaa gacgagcagc gtgtcttcct
    26941 tggcgatgcg gttgaaggtg acggccgtca cgccgcccgc ccacatcgcc gcggcgagga
    27001 gacccgtctg ccgcccgaag aagaaggccg cgagcaggaa gagagggaag accgtgagcg
    27061 cgccgaacac gacgttcggc agacgcagcg ccgcctcttc ggaaatctgc tgcccccgcg
    27121 cggcgcgcgc gttccacgcc ccggccgcct gcatcgagac gaagatcagc gtcttcatca
    27181 ccatcgggtg ctcggcgttc ggcgtgatgt cgccgcgggc gtaagcgcgc accgcgtcga
    27241 gcttgttgat ctcgtcctcg gcgaagccca cccgccccag ccccgccgcg cgcagataga
    27301 atccgcccac ggcgagcagc accagcaaca cgaccgcgta ccgctccatc gccgcgacga
    27361 gcctcaagcc ccccgcgcgc acgtccaacg ccagcgcgtc ctgtcgctca gaagctatgc
    27421 cgacgaactt agccataaca gtgagcggtg agcagtgagc agtgagcagt caagacgggt
    27481 tgcgctttct gactgctcac tgctcactgc tcactgctta ctcccactca tccttcagcc
    27541 ttcatcctta gcctcgtctc cgcgagcgtc tgttcggcgt cgcgcaccac gccgcgcacc
    27601 gagaggccgc gcccgacgag gaaggccagc ccgacggccg tccagacgag catccgcgcc
    27661 ttacgcacga tggccagcgt cacgcccgtg gagacgccga accgcaggac cttcgtgaag
    27721 agtcccgtcc cggcctcgtc gattcctacc ctgagcggca cgaatttgaa cactacgttg
    27781 atgacgcggt tgacggattc caggacgaag gccgcgagca cggtcggcgg ctcggcgctg
    27841 atgaaccaaa gcgttacgta ggcttcggcc acgccgagcg cgtggaagga ggcttcgagc
    27901 agcaggagcg ggaggaacat ccggcggttg cgcccgtaga aaccgtagac gcggtcttcg
    27961 agcgcgcgca gggtctcgcg ccgcgacccc agcgcccgcc gccccacccc gcgcgacgcc
    28021 agccgctcgg ccagcccgct caggatgcgc cactcggtgc gcaccacgta gaagccgacc
    28081 gtgaagacca ccgcggcgcc cacgatgatc ccgacgctcg tccagcgcag agcgcgcgtc
    28141 agcgggaagc tcaaaagcat cgccgccgcg ccgccgaaga cgaagagcac gaccgagaga
    28201 ctgtagaaga tgttctcgac cgtgatggcc gacatcgccg cgactaccgg cacccgttcc
    28261 cgcacgagca acaccttcgt cggctcgctc accaccagcc cgagcgggat gacgttgccg
    28321 atggcgtcgc ccgtcatgaa ggcccgcagc gcgtggcgca gtcgcaaacg gtgcggcgtc
    28381 tcgaagcaga gcgtccaggc gtaggagcgc accacgaagc gcaggcccga gagcgcgagg
    28441 atggcggcga agcccacccc gagtttttgc agctcggccc aaatcttctc gaccccggcc
    28501 tgccacacca cgtagccgaa gagcagcagc cccgccgcgg cgaagatcag gccggccggc
    28561 gcgagccgcc gcgccctgtc gctgccctta atctcttgag acatcacgcc acgcgcgccg
    28621 ctggcacccg ccccttcttc tgtccgcgtc gtgcggattt caacgtcttc tggaagatac
    28681 gccatcaatt aactttgctc aaagccccgg cgcacccggc agtcggccgc gggcgacaag
    28741 acccctcgcc cgctctctcg aagctgaaag ctgaccgccg agcgccgacc gcgcccacca
    28801 cgatttacga ttcacgattg acgatttacg gcttttgaga gttgacccga agcgccctga
    28861 taggcgatgc ttgggcgtcc ccgaaaattc taaccacagc gagacagcca actatgagca
    28921 gagcagcaca ggagatgttg agcgggagtc cgctgaccgt ctattccgag gacgaagaga
    28981 tgttccgcgc ctcggtgcgc gagttcgccg agggtgaaat ccgtccccgc gtcgaggaga
    29041 tggacgagca cgcgaagatg gcccccgaga tcatcaaaca gctcttcggt ctcggcctga
    29101 tgagcatcga gacgcccgag gagtacggcg gcacgggcgc caccttcttc acggccatca
    29161 tcggcgtcga ggaactctcg cgcgtcgatc cgtcggtggg cgttttgatg gacgtgcaga
    29221 acacgctcgt caacaacgcg ctcatccgct ggggcagccc cgagcagaag aagaagtatc
    29281 tgccgcagct cgccgccgaa aagttgggcg cctactgtct gagcgaagcc ggctccggct
    29341 cggacgcttt cgctctcgcg acgcgcgccg tcgatgaggg cgaccacttc cgcctgacgg
    29401 gccagaagct ctggatcacc aacggcaacg aggccgaaat cttcatcgtc ttcgccaacg
    29461 tccgtcccga agagggctac cgcggcatct ccgccttcgt cgtcgagaag ggcttcgacg
    29521 gcatctccgt cggcaagaag gaaaacaagc tcggcatccg cgcctcctcc accaccgaga
    29581 tcatcctcga caactgccgc gtgccgaagg agaacgtgct cggcgaactc ggcaagggct
    29641 acaaggtctc catcgagacc ttgaacgagg gacgcatcgg catcggcgcg cagatgatcg
    29701 gtctggcccg cggcgcactc gaacacgcgc tcaggtatac gggcgagcgc gcgcagttcg
    29761 gcaaacagat caaccagttt cagggcgtgc agttccaact cgccgagatg gccaccgaac
    29821 tcgaagccgc gcgtctcatg gtctataacg ccgcgcgcct caaggacgcc ggcctgccct
    29881 tcgtcaaaga ggcggcgatg gccaagctct actcgtcgcg cgccgccgaa cacatcgcct
    29941 ccaaggccat cgaactctac ggcggctacg gcttcgtcaa agactacccc gtcgaaaaac
    30001 tctggcgcga ctccaagatc ggcgccatct acgagggcac gtccaatatg cagttgcaga
    30061 cgattgcgaa gctgctcatc gggaagtgac ggcagcccgg tgataagtga tgagtgatga
    30121 gtgataagaa agactcttat ctcatcactt ctcatcactc atcacttatc actcatcact
    30181 ttgcttatgg ctacttcgcg catcgacatt ttccgcatga tgctccagag cgaccccgac
    30241 aacacggtcg tgtggttcgg gctggcgaag gagtgtgaga aggaggggcg tcacgacgag
    30301 gtggtcgagg cgctcaccga gtacctcgga cgcgccgatg acgagggcaa cgcctacggc
    30361 atgctggccc gcgcttacac cagcttgggc aaccgcgagg ccgcccgccg ctcctaccag
    30421 cgcggcgccg aggtcgccct cgcgcacggc cacccgtcga tggccgaaga gtaccgggcg
    30481 actttggaga cggaatttga ttaaaagccg tgaatcgtca atcgtgaatc gtcaatcgat
    30541 ttagttgacc gtcgtccgcc ttgagcaaca accgtcaagg cgagttgtta ttcaaccacg
    30601 attcacgatt gacgattcac gattgacgtt tttatcagga gagcagtcat ggcgaccgaa
    30661 ctgaaggcgc gtgagtatcg cggtttgagc cgtgaggaga tggtcggcat ctaccggacg
    30721 atgtacctgt cgcggcgcgt ggacgacaag gagattcagc tcaaggggca gaacaaaatc
    30781 ttcttccaga tttccggcgc gggccacgag gccgtgctcg tcgccgccgg ccgcgcgatg
    30841 aagccggcct acgactggtt ctacccctat taccgcgacc gcgcgctctg cctccagctt
    30901 ggcatgacgc cgctcgaaca actcctctcg gccgtcggct cggccgacga tcccaactcc
    30961 cacggccggc agatgccctc gcactggggc cacaaaaaac tcaacatcgt ctcgcagtcg
    31021 tcgcccacgg ggacgcagtt tttgcaggcc gtcgggtgcg ccgaggcgag cttccgcgcc
    31081 gcgctcatcg acgacatcaa ggacaaggtc gccggcttca aaggcgacga ggtcgtctac
    31141 tgttcggccg gcgacggcac gacgagcgag ggcgaattct gggaggcgat gaacacggcc
    31201 tgtaacctga agctccccgt cgtcttcctg atcgaagata acggctatgc catcagcgtc
    31261 cccgtcgagg tgcagacggc gggcggcgac atctccaaac tcgtctccgg cttccccgac
    31321 ctgctcatcc agaagtgcga cggcaccgac cccgtcgagt cgctggagac catgcgccgc
    31381 gccgtcgaat actgccgcca gcggaaaggc cccgcgctcg tccacgccaa agtcatccgc
    31441 ccttattcac actcgctctc cgacgatgaa aagctctacc gccccgacga agagcgcacc
    31501 tccgacgccg agcgcgaccc cgtcaaacgc ttcggcgcgc tcttggtcga tgaggggctg
    31561 gtcacacagg aagagcttca gcagctcaag gacgaggttg accgcgaggt gagcgaggcg
    31621 accgacctcg cgctcgccag cccgcagccc gcgcccgaga ccgccacaca gttcgtctac
    31681 tcgcccgacg ttgacccgac ctccgctgag ttcgacaccg aggcgggcgc cgaagtgtcg
    31741 ggcaacgccg gcacgatggt cgatctcatc aaccgctgtc tgcacgaaga gatggcccgc
    31801 gacccgcgcg ttctcgtctt cggtgaagac gtggccgact gctcgcgcga ggagaatctc
    31861 gacaaggtca agggcaaggg cggcgtcttc aaggtgacgg ccaacctcca gcgcaagttc
    31921 ggcggcgcgc gcgtcttcaa ctcgccgctc gccgaggcca acatcgtcgg ccgcgctatc
    31981 gggatggcca cgcggggctt gaagcccgtc gtcgagatac agttcttcga ctacatctgg
    32041 cccgcctatc accagatcag aaacgaactc gccctgatgc gctggcgctc gggcggcgac
    32101 tgggagtgcc ccatcgtcat ccgctgcccc gtcggcggct acctcaaggg cggcgcggtc
    32161 tatcactcgc agtccggcga cacgatcttc acgcacgtcc ccggcctccg cgtcgtctac
    32221 ccgtcgaacg ccctcgacgc caacggcctg ctacgcacgg ccatccgctg cgacgacccc
    32281 gtgctcttcc tcgaacataa gcacctctac cggcaggctt acaacaagtc gcagtacccc
    32341 ggcgacaatt ttctcatccc gttcggcaag gcccggacgg tgcgcgaggg cacagacctc
    32401 tccatcatca cctacggagc gctcgtccag cgctcgctcg tcgccgccaa acaggccgag
    32461 cagcaacagg gcgcgagcgt cgaggtcatc gacctgagga cccttgcgcc ctacgactgg
    32521 gaagcggtcg cgcgcaccgt ccgcaagacc agcaaggtca tcgtcgcgca cgaggacacg
    32581 cgcgcgcacg gcttcggcgc cgaggtggcc gcgcgcatct gcgacgagct gttcgactac
    32641 ctcgacgcgc ccgtccgccg cgtcgccgcc ctcgacacct tcgtcgccta cgcgcccacg
    32701 ctcgaagacc acatcctccc gcaggttgat gacgtgtata aagcgatcac ggagctttac
    32761 agctactaaa gcagattccg cgcacgcttc ggggcggaag cccgaccgtg agggagggcg
    32821 tcttgcacat tcagcacaga cgccctccct cacggtaggg cttccgcctt ttgccatcgc
    32881 gcgatacacg cctgtatcgt tccccgtcgc gctctctatg agcacccgct ccccttccct
    32941 cctaactgtt gttaggccac agcttcagcc cacaccctcc gcgtcgtagc ggcggcatga
    33001 aggttgccct attcatcgtg ttctgcacgt gtcagaacca cgcatatcca aaccaacaag
    33061 ggacaactta atcattcatc actcaatgga gggaaaaaga gagatgtgta aacgtttgct
    33121 gaatacagcg tgcgccgtcg cgctcgtttt cgccgcggcc gcggccgtca acgcacagga
    33181 cacaccgaag cccacgacca cggacacaca gaagccaacg actaccacta ccgtcaccac
    33241 gcagaccacg caggccgtcc agaacgcgga tgggacgtgg acggtcatcg aatacccggc
    33301 caacaaggag gtcgtcgtcg atctcactcc gggcctgaac atgacggcgg gcgccacggg
    33361 ccgcgccaaa gtccaccgca tggccgacca cacgatgctc cacctcgatc tctcgggggt
    33421 gaccggcgtc gaggcgctta acctctacgc ggttgaccca gcgggtcggc ccacgctcct
    33481 cggcccattg acgattcaca acggaacagt ctcgcaggac ttccgcacgc cgctcgacaa
    33541 gttcatgctc gtgctctcgc ccgaagcgag cctgacgact atggccgaca acacacccgt
    33601 cctcttccgc agcgccgtcc cgcagggctt cgccgtcgtg ccgaccgcga gccgcgaccg
    33661 cgaggacggg gccgtcgtgg gcgagaaggt ttcggccgtt tcgacctcgg gcggcacttc
    33721 ggcctactcg gtgccgatgc tcaatgtccc cggcatgaag cgcggcgacg actcggagat
    33781 caaggtcaac ctgacgggcg cgctcaccgg ctcgcgcgtc aacttcaaga tcgagccgcg
    33841 caaggacggc cccgtttcgg tcacggcccg cttccacgaa ctcaaggagt cgcctgcggg
    33901 gcaggtcttc gtcctctggg ccgtctcgcc ggacaacaag ttcaccaagc tcggacaggt
    33961 cgtcaacacc ggccgccgca acgaggcgga gattaaatcc gagaccactc tgacggactt
    34021 cggcctgctc atcacgctcg aagcaccgga ggccacgacg ccggccggcg ccgtcgtcgg
    34081 cacgatcatc cgtaactgac gagcgccgtc cgctcggacg ctcccgacgc cccggaggat
    34141 gcgacgcgcc cggcccgctc tatcggccac ggccgccgcg ccctccgggg cattttcctg
    34201 cccaaatgtg cgcccgcccg acttttcaca aaattgtcac agaaactctt gacttccctc
    34261 cccggaagcg cctaagctcg tctccagcag ggtcggcacc ctcgacgccg gcccgaaacg
    34321 ccacagggtt tcgcgccgaa atcccttccg catgtggccg tcctcgttcg cggccccaaa
    34381 ctcccagaag agttaaacgg aaatgttgaa acgcctgctt cccccctggc tcgcgctcgc
    34441 gctcgtgttt ctgtgcgcgg acgcggccgg cgcgcaacaa gacgctctcc ccgagctggt
    34501 ccggcgggtc aagccgtcgg tcgtctccgt catcacctac aactcgaagg gcgaggtgca
    34561 tgtcaccggc agcggcttct tcgtccgccc cgaccgcgtc gtcaccaacc tccacgtcgt
    34621 cgagagcgcg caccgcgtcg agatcaggac gttcgacggc aagggcaaga cctacgccgt
    34681 cgccgggctg ctcgacgccg acgaggaggg cgacctcgcg ctcctgagcg tgccggggtc
    34741 ggccgagcgg gcgcgggcgc tggaaacaag cgccgcgacg ccggaggagg gcgagcacgt
    34801 cttcgtcatc ggcaatcctt tgcgtctcga aggctcggtc tctgacggca tcgtctcggc
    34861 cgtgcgcgag gtgccttcgc tcgggcgcat catccagatc acggccccca tctcgcacgg
    34921 caactcgggc agccccgtct tcaacatgcg cggtcaggtc gtcggcgtcg tcaccatcaa
    34981 ggtgacgaac gggcagaaca tcaacctcgc catctcggcc gcgcgcctcg cctcgctgca
    35041 agagggcgcg cagacgctcg ccttcagtga gttcggcgcg cgcaagcgca cgggcgcgcg
    35101 cgagcctgac gccgtctccg agtggtggta caaaaacggg ctgaactcgc tctggctcgg
    35161 caactacgac agcgcgctca actacttcga gagcgccgtc tccaagaacc ccgagcgcgc
    35221 cgacgcgtgg atacaggtcg gcttctgcaa ggtcaaacag gggaagaact ccgacgccat
    35281 ccgcgccttc gagcgcgccc tgcgtctgcg gcccgactcc gtcgaagccc acaacaagct
    35341 cggcgacgcc tactactacg ccggcaactt ccaccaggcc atcgagtcct acaagcgcgc
    35401 cgtcgaactc cggcccgacc agtcagaggg ctattacaac ctcggcgtcg cctacctcga
    35461 agtcggcgac cgcccggcgg ccgtcgcgca ggcgcgcacg ctcaagagcc tcgactccga
    35521 actccaccgc aagctgctca acgagatcga acgctaactg agcgcggctc acgagtacca
    35581 atcaaaattc atcaagccct gaaggggcgg gataatcaaa tcccacccct tcagggcttg
    35641 atggttcttg caaacgtcgc tgatccaagg gcgttgttct cggctttcgc atgacgcccc
    35701 ttaggggctt ttctatgacg ccccttaggg gccggggatg cgcgtcagtt cctcctcggc
    35761 ccactcgcgg atgatgcggc tcatctcgtc gagttgtccc tcgaagaagt ggcccgcgcc
    35821 gggaatgacc tcgacgcgtg tgcgcgcttc cggcggagtc tgcgcggcga gcgagcgcac
    35881 gtcggcggcg gcgccgaact cgtcgcgctc gccgtggacg agcaggaggg ccttacggca
    35941 ctcgcgcagg aacgagaagt catagtcgcg ctcgtccatc ccgattggcg taccgatgcc
    36001 gatgagcgcg ccgacgcgcc cgtcgcgcgt gccgacttca aggccgacgc gcgcgccgaa
    36061 ggagaagccg gcgaggaaga cggggaggcc ggggtaagcg cccgtgaggt ggtcgagcgc
    36121 cgcgcgggcg tcctcctgtt cgccgccgcg ggcgtggtcg tgcgcgccgg tcgagtgtcc
    36181 gacgccgcgg aagttgacgc ggagcgtgac gagtccggcc tcgccgaggg cgcgcgccgc
    36241 gcggaagacc accttgttgt gcatcgtccc gccgtgcagc gggtgcgggt gcagcaccag
    36301 cgcgacgccg cgcgccgtgg ccgccgcgcg cggctccttc aagatcgctt cgaggcgccc
    36361 gtgcggggcg gggatgaaca ggtttccggc agggtacatg gtgcgtagtg agcagtgagc
    36421 agtgagcagt tgctcctgaa tcgccttgct gcctgctcca cagcgccctt gtttgtatca
    36481 aaatcgcggc gcgttggcga actgcgggaa gcgcccggcg gctcgctaat ttcaactgct
    36541 cactgctcac cgctcactgc tgacctcttt cttgacttgc atttttgaaa gcagtagctt
    36601 gaatgaaggc gctctctagt gccgcagtcc acttgccgtg gcgactccgc acagtcaatt
    36661 tgtcgccgcg gcgcgagaat ccgcgaaaga gcaaagggtc aatcggtgac agacatcgca
    36721 gacagcggcc ggcaacaggc agcgccccag atgcgcgctc gcccgacgcc cgcgtccgcc
    36781 ctccggcgct acggcctcgc ggccggctcg gtcgcggcgg ccacgctgct gatgttcgcg
    36841 ctctactacg tggtcggact cgcgcgcggc agcgtcccgt tcatcttcta cttcggcgcc
    36901 gtcatcctga gcgcgcgtta cgccggccgc ggccccacgc tgctcaccat cctcctctcg
    36961 gcgctcgccg ccgactactt cttcatcccg cccttcaact ccttttctcc caacgccgag
    37021 agcgtcattc aggtcggcgt cttcgccctc gtctcactct tcatcgcgca cgtcaccagc
    37081 cgcagcgtcc gctccgaggc cgccgcgcag aagagcggcg aggagttggc gacgacgctc
    37141 cgcagcatcg gcgacgccgt catcacgacc gacgacgcgg ggcgcgtgac gtttatgaac
    37201 ccggtggccg agcgcgtcac gggctggacg ggtgcggagg cgcgcggccg cgacctgatg
    37261 gaagtcttcc gcatcgtcgg cgaagggacg cgcgagccgg tcgagaatcc ggccacacgc
    37321 gtgttgcgcg aaggggcggt cgtcggcctc gccaaccaca ccgtcgtcgt cgcccgcgac
    37381 ggccgcgaga cgccgattga cgacagcgcc gctcccatcc gagaccgcgc cggcaaagtc
    37441 gtcggcgtcg tcctcgtctt ccacgatgtc accgagcgcc ggcaggccga ggcgaacatc
    37501 cgcaagagcg aggagcgcct gcgcgccgtc gccgagaccg cggccgacgc catcatcacc
    37561 atcgacgagc agagccgcat cctctacgtc aacccggcgg ccgggagaat cttcggccac
    37621 gcgcccgccg acattctggg ccacaacctg accatgctca tgcccgacta cctgcgacgc
    37681 gcccacgagg cgggcctcgg caggtacgtg gggacggggc agcgccacat ttcgtgggag
    37741 ggcgtcgagc tgcccggtct gcaccgcgac ggccacgaga tttctctgga agtctccttc
    37801 gccgagttcg tcgaggacgg ccggcgcttc ttcacgggca tcgcgcgcga catcacggag
    37861 cgccggcgcg ccgaggaggc gatcacgaaa ctggcggcca tcgtcgagtc gtctcaggac
    37921 gccgtcatcg ggaagacgct cgaaggcacg atcacgagct ggaacgcggc cgccgagcgg
    37981 ctctacggct acacttccgc ggaggccgtc ggccgacaca tctcgatggc cgccccgccc
    38041 gagcgccggg acgagatcga cgagattctg agccgcatca ggaacggcgc gaaggtcgag
    38101 catctggaaa ccgtccgcgt gcgcaaagac gggacgcgct tcgacgtttc cgtcacagtc
    38161 tcgcccatca gaaattccgc gggcgtcgtc accggcgctt cgaccatcgc gcgcgacatc
    38221 accgagcgca cgcgcgccga ggagtcggtg cgcgcttctg aagaaagtta tcgcgcgctg
    38281 gccgacgcca tgccgcagat cgtctggacg gcgcgccccg acggctacta cgactactac
    38341 aaccgccgct ggttcgaata caccgggatg acgatggagc agaccgaggg gtggggctgg
    38401 cagcccgtgc tccacccgga cgacgtggag ctgtgcctgc gaacatggtc gaacgccgtc
    38461 gtctccggcc acggctacga gatcgagtac cgcttccgcc gcgcctccga cgcgcagtac
    38521 cgctggcacc tcggccgcgc cgagccgatg cgcgacgccg agggacgcat cctcaagtgg
    38581 ttcggcacgg ccaccgacat ccacgaccag aagcagtcgg ccgaccgctc ccgcttcctc
    38641 gccgaggccg gcgccatcct cgcctcctcg ctcgactacg aggccacgct cgaacgcctc
    38701 gcgcgcctca ccgtgccggc gctcgccgac tactgcctga tcgacatcgt ggacgaggac
    38761 aacgtggtgg cgcgcgtggc gacctcgcac gccgacccct cgaaggagaa gttgatcgag
    38821 cgcctgcgcg cctacccgcc cgatttgtcg aacgacacgg gcgtgcccgg cgtgctacgc
    38881 acgggccggc cctcgttcgt cgccgacgtg acggaggagg tgctgcgttc cgtgacccgc
    38941 gacgacgctc accgcgaact ccttcgccag atgggcctca aatcctacat gaccgtcccg
    39001 ctcgtcgcgc gcgagcgcat cgtcggggcg ctcaccttcg cctcgaccac cgaccgtctc
    39061 cggtacggcc ccgaagacct ccccttcgcc ggggaggtgg cgagcagcgc ggcgctcgcc
    39121 atcgacaacg cgcgcctcta tcgcgcctcg cgcgacgcca accgctccaa ggacgagttt
    39181 ctcgccacgc tctcacacga gttgcgcacg cccctgacgc ccatcatcgg ctggacgcac
    39241 atgatgcgcg ccggccgtct gcccgaggcc gaggccacac acggactcga agtcgtcgag
    39301 aaaaacgcgc gctccttaac gcgcctcatc aacgacctgc tcgacatgtc gtcgatcatg
    39361 agcggcaaga tgagcatcga gcgcgcgccc gtcgatctcc gacaggcgct cgccgaggcc
    39421 atcgaaactg tgcggccgct ggccgagcag cgcggcgtcc gactggaact cgccgccacc
    39481 gacgacgcgg acgcgcccgc aatcatctcc ggcgaccgca cgcggctcgt gcagatcttc
    39541 tggaacctcc tcagcaacgc ggtgaagttt tccggcgcgg gcgcgcgcgt gagcgtcggc
    39601 tgccgcgccg acaacggcga ggcgtgggtc gaggtggcgg acgagggcga aggcattgcg
    39661 cccgacttcc tgccgcacgt cttcgagcga ttccggcagg ccgacggcac gacgacgcgc
    39721 gcgcacggcg gcctcggcat cggcctcgcc ctcgtcaaga gcctgacgga agcgcacggc
    39781 ggccagatca cggccgagag ccggggcgcg gggcgcggca gccgcttcgt cgtccgcctc
    39841 ccgctcctgc gtgcgccgga cgcggacgac gaagcgccga cggaaggcgc ggccggagcg
    39901 cgcgcgacgg ccagcccgca gccttccgtc ccgaaggccc gcgtcctcgt catcgaagat
    39961 tcgcgcgaca cgctggagat gctgcgcgtg gtactctcga cacgcggcta cgaggtcgtc
    40021 gcctgcgagt cggccgccga ggcgttgcgt ctcgccccgg cgatgtgttt tgatgtcatc
    40081 gtctctgaca tcggactccc tcgcgtggac ggctacgaac tgatcgggcg cctgcgccag
    40141 ctcgcgcacc tgagcgcgac gccggccgtg gcgctgacgg gctacgcttc cacgaaggac
    40201 gcgcaggccg ccctcggggc cggcttcgat atgcacgtcg cgaagcccgt cgatccggcg
    40261 accctcgtcg gcgccgtcga gcgtctcttg aagacacgcc cggcgacggt ctcgaattcc
    40321 gaatcctgac ctcgcgttgg ttgtaatctg aaatccaaag gtcggcggcg acgcctgtcg
    40381 ggtctgagct ttttgtctgc ccctgctgca agaatctcag ccccgctcgt cacgcgagtc
    40441 agggcgtcgg cccggcggcc tttttatccg agggagaaag tgatggaagg attgaccccc
    40501 acggcgacga gtcaggactt tttcacacac caagcgggct cgcccaaccc ttacatgtcc
    40561 ggcgccccgg ccgacccggc cgccattccg cgccccgccg gcgacgacca ccagaccgcg
    40621 aaggccaaac cgcgcgccct cgtcgtggac gacgcgcccg acgtaaccga gatgctcgcg
    40681 atgcttctgc gctacgccgg ctacgacgtg gtgacggtct tctgtgcgcc cgacgcctac
    40741 gagaccgcgc tctccgagca gttcgacgtg gtggtctccg acatcggcat gcccggcatg
    40801 aacggctacg agctggccga gcgcctgcgt agtctccccg actacgaggc cgtgccgctc
    40861 atcgccgtca ccggcttcgc catgtatgac gaccgcgagc gcgccacgca cgccggcttc
    40921 gacgccttcc tcaccaagcc gatcaacccg agcgacctca tcaacctcgt caagcgtctg
    40981 agcgacggct aagtaaggca aaagtaaaaa ggcaaaaggc aaaagtgaga aggcataacc
    41041 gccgtcttca cttttgcctt ttgccttttt acttttgcct tacttacagg tcgtcctgcg
    41101 aggagtcggc ggcgtcgagc gcgcagacga cccagcgcgc ctcctccgac tcgaactcgt
    41161 cggcctgatg ttcgagcgcg tcgcgcgcca cctccgcgtc cacggggtcg gcgagcagtt
    41221 ggacgaggac gccgacgggc agttgcagca gcgcctcgtg ggcgatagcg cgcacggcct
    41281 gattgtcgtg gcgggcgagt tcgcacgtct cgctcggcgc gaggcgcccc gaaagcgcgt
    41341 cggcgagggc atcgacgcgc gtgtagtcgc gcgcccgcag cgcccgctcc agcagttgag
    41401 cgaagacctc ggccgagcgc gcctcgcgat gcagcacgcg cgcacacgtc tcggccacct
    41461 gcccgagcgc acacgcctgc tcgccgctca ggcccgcgtc gccgccgtcg gccgcgcgcg
    41521 cgtcgagcgc ggccagcagc cggctcaact cccactcgga gcaggcgtcg tagccggtgc
    41581 gctgaacctt agcgcccccg ccggcgacct gtgtggcgcc gagcgagcgc accacgagac
    41641 tgcgcgtcgc cggcctcaag ccctgccaca cgctcgccac gctcgccccg aactcttcaa
    41701 tcaacattca aatgttctcc agcctgacac acgcggcggg gcgtcgtcgc cccaagcaac
    41761 gcgcatacta taaggtggcg gattgcgtga gacaagcaca aaatatggcg gcgtgaaagg
    41821 ccgtgaatcg taaatcgtaa atcgtaaatc gtgataacct gcttccactc agccttcctc
    41881 acttctgaaa ggagtcttcc atgcgacctc acgaaaatct tgacgcttgg gtcaaggcca
    41941 tcgattttgt cgtaactgtc tacaaaatca ccgagccatt cccgaaggaa gagaaattcg
    42001 gcctcacctc acaaatcagg cgcgccgccg tttcagtacc ggctaacatc gccgagggag
    42061 cggcgcgtag gtctccaaag gagtttctgc acttcctttc caatgcacag ggttcagcaa
    42121 gcgaagttga gacagagctt ctaatcgccc accgtttggg ctacctggag agtacggagt
    42181 atctcaaact ccgttcgtcc ctcagcgaca tcggtcgaat gctcaccggc ctgtcgcagc
    42241 acctcaaacg gaaagactcg tgaatcgtca atcgtgaatc gtggttgaat cacatcctgc
    42301 ctgagtgtca gcactcaagc ccgaaggagg tcaactgaat cgatttacga tttacgattc
    42361 acgatttacg agttctattc cgccgtgggt gatggcgagc atgatgcaat cttcgccggt
    42421 gtcttcgagg gcggtggggt gatgtgcgga gccgggttcg ttgaggacgt agtcgccggc
    42481 gcgatgcggg ccgcggtcgt ccgtgtagcc gccctggagg atgagcactt cttccagccc
    42541 ccggtggcgg tgggccgggt aggagcagcc cggctccatg cggatgagga cggtggcgtc
    42601 gccgcttcgc tcgtcgcgcc gcagcacgcg gaggtggatg ccgcggtggc gcgtcggc
//
LOCUS       DASVES010000137         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1694464, whole genome shotgun sequence.
ACCESSION   DASVES010000137 DASVES010000000
VERSION     DASVES010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694464"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /gene="rplA"
                     /locus_tag="VF240_05095"
     CDS             complement(<1..575)
                     /gene="rplA"
                     /locus_tag="VF240_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7174641.1"
                     /translation="MAAHGKKYRAALEKIEFGRKYTLEEGLSKVREVSAVTARKFDET
                     VELTMWLGVDPRKADQLVRGTVVLPHGIGRVTRVLVITQGDKLREAEAAGADFVGGED
                     VVARIKNENWLDYDTVIATPDMMRFVGQLGKILGPRGLMPNPKTGTVTPDVTTAVRET
                     KAGKVEYRVDKTGVIHVGIGKVSFEPDKLRE"
     gene            complement(720..1148)
                     /gene="rplK"
                     /locus_tag="VF240_05100"
     CDS             complement(720..1148)
                     /gene="rplK"
                     /locus_tag="VF240_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7174642.1"
                     /translation="MAKKITGYIKLQVPAGKANPAPPIGPALGQHGVNIMEFCKAFNA
                     KTAQMDPDLKIPVVITVFADRSFTFETKTPPAADLLKKAAGVPKGSGNPKKDKVGSIS
                     RDKIEEIARLKLQDLNTTNLESAVKTIEGTARNMGLTIEG"
     gene            complement(1233..1793)
                     /gene="nusG"
                     /locus_tag="VF240_05105"
     CDS             complement(1233..1793)
                     /gene="nusG"
                     /locus_tag="VF240_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX7174643.1"
                     /translation="MKQWFILHTYSGHEKKVRDSLKARVRDTDLQSEITEVLVPTETV
                     VEMRGNKRVESSRMFYPGYVLVQIECDERGHIPDDVCHLVKSTPKVTGFVGGQHPTPL
                     TPDEVNQIVHNVTEAAEKPKPKFTYTPGETVRIKQGPFQSFTGKVEDVNADKSLLKVV
                     VTIFGRSTPVELNFLDVEKVTFTEEE"
     gene            complement(1821..2168)
                     /gene="secE"
                     /locus_tag="VF240_05110"
     CDS             complement(1821..2168)
                     /gene="secE"
                     /locus_tag="VF240_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX7174644.1"
                     /translation="MTDERDDSEKPESAGSSVDVSPRATPFGAGPEGRPARRERAEST
                     PPRSNPFERAGKFIRDTRGELRRVSWPTRLEVRNTTIITIIAVIFFAVYLWVVDKGFT
                     LLISQLQKLLGAA"
     gene            complement(2229..2301)
                     /locus_tag="VF240_05115"
     tRNA            complement(2229..2301)
                     /locus_tag="VF240_05115"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2265..2267),aa:Trp,seq:cca)
     gene            complement(2381..2533)
                     /gene="rpmG"
                     /locus_tag="VF240_05120"
     CDS             complement(2381..2533)
                     /gene="rpmG"
                     /locus_tag="VF240_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7174645.1"
                     /translation="MPRDNIVLQCTECKSRNYVTTKNKKTTTGRLEFRKFCRRCRLHR
                     AHKETK"
     gene            complement(2581..>2925)
                     /gene="tuf"
                     /locus_tag="VF240_05125"
     CDS             complement(2581..>2925)
                     /gene="tuf"
                     /locus_tag="VF240_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7174646.1"
                     /translation="GVERKDIERGQVIAKPGSITPHTVFKAEAYVLTKEEGGRHTPFF
                     TGYRPQFYFRTTDVTGVATLPEGVEMVMPGDNVAMEITLITPIAMEKGLRFAIREGGR
                     TVGAGTISDIVE"
BASE COUNT          471 a          944 c          863 g          647 t
ORIGIN      
        1 ttctcgcgca gcttgtccgg ctcgaacgac accttgccga tgccgacgtg gatgactccc
       61 gtcttgtcca cgcgatattc gaccttaccg gccttcgtct cgcgcacggc ggtcgtcacg
      121 tcgggcgtca ccgtccccgt cttcgggttc ggcatcaggc cgcgcgggcc gaggattttg
      181 ccgagttgtc cgacgaagcg catcatgtcg ggcgtcgcga tcaccgtgtc gtagtcgagc
      241 cagttctcgt tcttgatgcg cgcgaccacg tcctcgccgc cgacgaagtc cgcgccggcc
      301 gcctcggcct cgcgcagctt gtcgccctgc gtgatgacga gcacgcgcgt cacccgcccg
      361 atgccgtgcg gcagcacgac cgtgccgcgc acgagttgat cggccttgcg cggatcgacg
      421 ccgagccaca tcgtcagctc gaccgtctcg tcgaacttcc gcgccgtgac ggcggagact
      481 tccctgacct tcgacagacc ctcttcgagt gtgtacttcc gcccgaactc gatcttctcg
      541 agcgccgcgc ggtacttctt gccgtgtgct gccatttaac ttcctccgag gtgcaagcgg
      601 agcggacgac ggccgcgcct ccctcttcaa gggggatgag ggcggaggga tgagggatga
      661 acggttgagt gtaaagcgtc ggttaagccg gtcactcagt cttgttcatc cctcatccct
      721 catccctcga tggtgagtcc catgttccga gccgtgccct cgatggtctt gacggccgat
      781 tcgaggttcg tggtgttgag gtcctgaagc ttgagccggg cgatctcctc gattttgtcg
      841 cgcgagatcg agccgacctt gtccttcttc gggttgcccg agcctttggg cacgccggcg
      901 gccttcttga gtaggtcggc cgcgggcggc gtcttcgtct cgaaggtgaa ggagcggtcg
      961 gcgaagacgg tgatgacgac cgggatttta aggtccgggt ccatctgcgc ggtcttcgcg
     1021 ttgaacgcct tgcagaactc catgatgttg acgccgtgct ggcccagcgc cggcccgatg
     1081 ggcggcgccg ggttggcctt ccccgccggc acctgcaact tgatgtaacc tgtaatcttc
     1141 tttgccatat gtcttagtga tgagtgatca gtgataagtg ataagataag cgccttgtct
     1201 tatcacctat cacttatcac tcatcactgc tcttattctt cctctgtaaa cgtcaccttc
     1261 tcaacgtcga ggaagttgag ttcgaccggc gtcgagcgcc cgaagatggt gacgaccacc
     1321 ttgaggagcg acttgtcggc gttcacgtcc tcgaccttcc ccgtgaagct ctggaacggc
     1381 ccctgcttga tacgcaccgt ctcgcccggc gtgtaggtga acttcggctt gggcttctcg
     1441 gccgcctccg tcacgttgtg gacgatctgg ttgacctcgt ccggggtgag gggcgtcggg
     1501 tgctgcccgc cgacgaagcc cgtcaccttc ggcgttgact tgacgaggtg gcatacgtcg
     1561 tcggggatgt gcccgcgctc gtcacactcg atctgcacca gcacgtagcc ggggtagaac
     1621 atgcgggagg attcgacgcg cttgttgccg cgcatctcga cgacggtctc ggtcgggacg
     1681 agcacttccg tgatctcgct ctggaggtcg gtgtcgcgca cgcgcgcctt gaggctgtcg
     1741 cgcaccttct tctcgtgccc ggagtacgtg tgtaggatga accactgctt catacggaaa
     1801 cttccccgcc gccctcggct tcaggcggcc ccgagcagct tctgcaactg cgaaatcagg
     1861 agcgtgaaac ccttgtccac gacccacagg tagacggcga agaagatgac ggcgatgatg
     1921 gtgatgatcg tcgtattcct gacttcgagg cgggtcggcc aggagacgcg ccgcagttcc
     1981 ccgcgcgtgt cgcgtatgaa cttgcccgcg cgctcgaacg ggttgctgcg cggcggcgtg
     2041 gactcagccc gctcgcgccg cgccgggcgt ccctccggcc ccgcgccgaa aggcgtcgcg
     2101 cgcggcgaga cgtcaactga cgaacccgcg ctctccggct tttccgagtc gtccctctca
     2161 tcggtcatcc gctcttcctc ttttctcgac accccgactc taccttcccg gactccaaac
     2221 ggtgacgaca ggggtaacag gatttgaacc cgtactttcg gttttggaga ccgacgtgct
     2281 aaccgttgac actatacccc taaggaaggt gatgagtgac aagtgacgag tgataagaaa
     2341 aactgtcttt ctcatcactt atcactcatc acttatcacc ctacttggtc tccttgtgcg
     2401 ccctgtgcag acggcaacgg cggcagaatt tcctgaattc gagacgcccg gtggtcgtct
     2461 tcttgttctt cgtcgtcacg tagttgcggc tcttgcactc ggtgcattgc aacacgatgt
     2521 tatcgcgcgg cataacttaa cctctctgat taaagcctgt gattcaaaaa caaagggcgc
     2581 ttactcgacg atatcggaga tggtgccggc gccgaccgtg cggccgccct cgcggatggc
     2641 gaagcgcaac cccttctcca tcgctatcgg cgtgatcagc gtgatctcca tcgccacgtt
     2701 gtcgccaggc atcaccatct ccaccccctc gggcagagtc gccacccccg tcacgtccgt
     2761 cgtccggaag tagaactgcg gccgataccc cgtaaaaaac ggcgtgtgtc tgccgccctc
     2821 ttctttggtg aggacatacg cctcggcctt gaagaccgtg tgcggcgtga tgctgcccgg
     2881 cttggcgatc acctgcccgc gctcgatgtc cttgcgctcc acgcc
//
LOCUS       DASVES010000138         6878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1214961, whole genome shotgun sequence.
ACCESSION   DASVES010000138 DASVES010000000
VERSION     DASVES010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214961"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF240_05130"
     CDS             complement(<1..387)
                     /locus_tag="VF240_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7174647.1"
                     /translation="MRFPFTLRADCEFDAVGFGLNAVDHLIVVPAYPEFDTKTRLVTH
                     QQSAGGQTASAMVALRRLGMRTAYAGRFGSDDEGAFGLRALEDEGVNVEYAQVIDGAR
                     NQIAFIVIDARTGERTIIWDRDERLAY"
     gene            complement(562..3948)
                     /locus_tag="VF240_05135"
     CDS             complement(562..3948)
                     /locus_tag="VF240_05135"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7174648.1"
                     /translation="MIRTRRLLALALALTLFPASAARPAFAQDSGNAVVVAAAPYFTE
                     PALSPDRSEIAFVSGGDIWTAPADGGAARLLVAHPADERRPVYSPDGRQLAFTSTRTG
                     NGDLYVLSLDAGELRRLTFDDGQDLLDAWSADGRWLYFSSTSRDIAGMNDIFRVSPSG
                     GTPMQVSADRYTNEFFSAPSPDGAALAFSARGISSGQWWRKGSSHIDESEIWVARGLD
                     GAGAAPTYEQVAERGARQSWPMWGSDGRRLFYVSDRGGAQNIWSRVPGQQARQVTSFR
                     DGRVLWPSISRDGRLIVFERDFRLWKLDTESGRAAEIQITRRGATSGPAVEHVRLSDN
                     FNEFALSPDGKKVAFIVRGEVFAASSTDGGDAARVSQTPQEESQVTWSPDSRRLAYVS
                     DRDGIAHLYSYDFATNKETQLTSGAQPDDTPRYSPDGKLLAFQRDGKELRVLTLASKQ
                     ERLVASGTFDRPPVNPDRPFAWSPDSKWIAYAPIADKLFRNLYVVAAEGGAAPKPVSF
                     LANTGGAGTVSWSPDGTYLLFNTGQRTEAGQIARVDLIPRTPKFREDQFRELFREETP
                     RNLTPRPAEQERPAPTQTEGPPAPTPTPAATPQAAPSPSASPAASPATEGQAARPGPT
                     PAPKPVEVVFEGIRERLSLLPVGIDVNSQAISPDGKWVVLVASVANQSNLYVYSLDEL
                     AREPAVARQLTSTTGFKAQAQFSPDSKEVFYLEAPGRIGVVNLEGRTRPLALTAEMDV
                     DFSTQKAQVFTQVWTYLRDHFYDPGYHGADWTRLRADYEPRVAGARTPEEMRRLLNLM
                     IGELNASHLGVSGPGAAVVTSTGRLGLRFDRAEYEQRGRLRVSEVIPLSPAALGGSIR
                     PGDYLLAVDGRTLDARTNLDELLNFKINRRVTLTVASSADGANRREVAVRPSNSATEK
                     ALLYRKWVEDNRAYVARISNGRLGYVHMFDMSSGSLAQLHVDLDAENHAREGVVVDVR
                     NNNGGFVNVYAIDVLARRGYLTMTPRGQGSASGRTVLGQRSLERPTVLVTNQHSLSDA
                     EDFAEGYRTLRLGKIVGEPTAGWIIYTWNTGLIDGGTVRLPRMKVTDNTGADMELAPR
                     PVDVPATRPVGESYAGKDSQLDAAVRELLGQLGERTSRRE"
     gene            complement(3977..4918)
                     /locus_tag="VF240_05140"
     CDS             complement(3977..4918)
                     /locus_tag="VF240_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174649.1"
                     /translation="MKPDFPVWARGRRRRRRGAALAAAVVVSLLRAHAVCAQEGAHAR
                     GAVPDKLWQGLQGPVKTVRTEEFRVEMKDGREVSRRVNTKTDNYDAKGFMTESFDGDE
                     PEAARFVYRQDVRGRPVETTLYLGGLRFGRDTHSYEASGRRVESHSYDSGGRLRKREV
                     TTLDERGNDVRREIEHFDEDGKLTGEKSVLVYAYTYDVGGRLTESTSGDERGRLSDKY
                     VSVYDASGRRTASTTYSYDQQSRALLTKTVFAYDARGDTVTVHAYDPRGALIYREAHA
                     YEYDARGSWVTERRTTWHTEHAPASVTLIRRRTISYY"
     gene            5039..>6878
                     /gene="uvrC"
                     /locus_tag="VF240_05145"
     CDS             5039..>6878
                     /gene="uvrC"
                     /locus_tag="VF240_05145"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7174650.1"
                     /translation="MTLEEKLKNLPVSPGVYLHKDEAGKIIYVGKAKNLRSRVRSYFQ
                     SGRGHDRKTRELVRRVRDLEFIVTDNEVEALVLESNLIKQHKPRYNVLLKDDKQYPHL
                     KLTINEPFPRVMITRRIQRDGALYFGPFLPASLARRTIDLINRTFQLRTCDIEIDGRL
                     PRPCLEYHIKRCLGPCVKGLCTPEEYKESVRDVRMFLEGRNRELADEYEGRMAQASDE
                     MRFEMAAKYRDLRKVVLAVSEQQKMATTSERDVDIFGYYREGPRLALQLFTMREGRIV
                     GRREFFWEDLSEDEFDPAKFLGEVLTQYYSTDYVPREVHVPSDFDDRELLERALTDRK
                     GRRVRILDPRRGEKRDMIELVEKNAKIAFEQRFRVLKPDMERVLEELQETLELPRFPS
                     RIESFDVSNISGAENVASMVVCENGKMNRAEYRKFKIKTVEGANDPASMREAVLRRYR
                     RQLDERNALPDLVMIDGGITQLNASASAMRELDLEAIPVVGVVKPPRRHNEVSHLLVK
                     GREHEPVFIDSHSPVLRLIQMIRDETHRTAVAYHRKRRELRDFTSELTAIPGVGDKRK
                     NRLLRNLGSIQRIAQAGVAELTPFVGAKTAAEIVEHFTRQRALAESG"
BASE COUNT         1124 a         2261 c         2376 g         1117 t
ORIGIN      
        1 gtaggcgagg cgttcgtccc tgtcccagat gatcgtgcgc tcgccggtgc gcgcgtcgat
       61 gacgatgaag gcgatctgat tgcgcgcccc atcaatcacc tgcgcatact cgacgttcac
      121 gccctcgtct tcgagcgcgc gcaagccgaa agcgccctcg tcgtcggagc cgaagcggcc
      181 ggcataggcc gttcgcatgc cgagtcgtcg gagcgcgacc atcgcggagg ccgtctgccc
      241 gccggccgat tgctggtgcg tgacgagccg cgtcttggtg tcgaactcgg ggtaggcggg
      301 gacgacgatg aggtgatcga cggcgttgag gccaaaaccc acggcgtcga actcacagtc
      361 cgcgcgaagc gtgaatggga agcgcaagcg tcgtgtactc ctctcgtggc ggccgcttca
      421 gcgccgacgc ggcgtgacga cttcgttgaa gcttcctcgg ttcttgagag cagggcgacg
      481 ggcgttctct gcgcctctgc ggtgagtgtt gttgcgtcac gctcaagcaa aaagcaaagc
      541 gtcgcgcgct ttacggtctg tctattctct cctgctggtg cgctccccga gctggccgag
      601 cagttcgcgc acggccgcgt cgagctgcga atctttgccg gcgtaggact cgccgacggg
      661 gcgtgtggcg ggcacgtcca cggggcgcgg ggcgagttcc atgtcggcgc ccgtgttgtc
      721 agtcaccttc atgcgcggca ggcgcaccgt gccgccgtcg atgaggcccg tgttccaggt
      781 gtagatgatc cagccggcgg tcggctcgcc gacgatcttg ccgagacgca gggtgcgata
      841 gccctcggcg aagtcttcgg cgtcggaaag cgagtgctgg ttggtgacga ggacggtggg
      901 gcgctcaagc gagcgctggc cgaggacggt gcggcccgag gccgacccct gaccacgggg
      961 cgtcatggtc aggtagccgc ggcgggcgag cacgtcgatg gcgtagacgt tgacgaagcc
     1021 gccgttgttg ttacgcacgt caacgaccac tccctcgcgc gcgtggttct cggcgtcgag
     1081 gtcaacgtgg agctgcgcga gcgaccccga agacatgtcg aacatgtgga catagccgag
     1141 ccgaccattg cttatgcgcg cgacgtaagc gcggttgtcc tcgacccact tacgatagag
     1201 cagcgccttc tccgtcgcgg agttggaggg gcgcacggcc acctcgcgcc ggttcgcgcc
     1261 gtcggccgac gacgccaccg tcagagtcac gcgccggttg atcttgaagt tcaaaagctc
     1321 gtcgaggttc gtgcgcgcgt cgagcgtgcg gccgtccacg gcgagcaggt agtcgccggg
     1381 gcggatgctg ccgccgagcg cggcggggct taaggggatg acttcgctga cgcgcaggcg
     1441 gccgcgctgc tcatactcgg cgcggtcgaa gcgcaggccg agccggcccg tcgaggtgac
     1501 gacggcggcg ccggggccgg agacgccgag gtgcgaggcg ttgagttcgc caatcatcag
     1561 gttgagcagg cggcgcatct cttcgggcgt gcgcgcgccc gccactcgcg gctcgtagtc
     1621 ggcgcgaagg cgcgtccagt cggccccgtg gtagccgggg tcgtagaagt ggtcgcgcag
     1681 gtaagtccag acctgtgtga agacctgcgc cttctgcgtc gagaagtcca catccatctc
     1741 ggcggtgagc gcgagagggc gcgtgcggcc ttcgaggttg acgacgccga tgcgccccgg
     1801 cgcttcgaga tagaagacct ccttgctgtc gggcgagaac tgcgcctgcg ccttgaagcc
     1861 ggtcgtggag gtgagctgac gggctacggc cggctcgcgc gccagctcgt cgagcgagta
     1921 gacgtagagg ttcgactggt tcgccaccga cgcgaccaac acgacccact tgccgtccgg
     1981 gctgatggcc tgggagttca cgtcgatgcc gacgggcagc aaactcagcc gctcgcggat
     2041 gccctcgaag acgacctcga cgggcttcgg cgcgggggtc gggccgggcc gcgcggcctg
     2101 tccttcggtt gcgggtgaag cggcgggcga ggcggaaggt gaaggcgcgg cttgcggggt
     2161 tgcggccggc gtcggagtcg gcgcgggcgg cccctcggtc tgcgtgggcg cggggcgctc
     2221 ctgctcggcg gggcgcggcg tcaggttgcg cggcgtctcc tcgcggaaga gttcgcggaa
     2281 ctgatcctcg cgaaacttgg gcgtgcgcgg gatgagatcg acgcgcgcaa tctgcccggc
     2341 ttcggtgcgc tgccccgtgt tgaagagcag gtacgtgccg tccgggctcc acgagacggt
     2401 gcccgcgccg ccggtgttcg ccagaaaact gacgggcttc ggcgcggccc cgccctcggc
     2461 cgcaacgacg tagaggttgc ggaagagctt gtcggcgatg ggcgcgtagg cgatccactt
     2521 cgagtcgggc gaccacgcga acgggcggtc ggggttgacg ggcgggcggt cgaaggtgcc
     2581 ggaggcgacg aggcgctcct gcttcgacgc gagcgtgagc acgcgcagct ctttgccgtc
     2641 gcgctgaaag gcgaggagct tgccgtcggg cgagtagcgc ggcgtgtcgt caggctgtgc
     2701 gcccgaggtc agttgcgtct ccttgttggt ggcgaagtcg taggagtaga ggtgtgcgat
     2761 gccgtcgcgg tcggagacgt aggcgaggcg tcggctgtcg ggcgaccaag tgacctgcga
     2821 ctcctcctgc ggcgtctgtg agacacgcgc ggcgtcgccg ccgtcggtgg acgatgcggc
     2881 gaagacctcg ccgcggacga tgaaggcgac cttcttgccg tcgggcgaca gcgcgaactc
     2941 gttgaagttg tctgagagcc gcacgtgctc gaccgcgggg ccggaggtcg cgccgcgccg
     3001 cgtgatttga atttcggcgg cgcgccccga ctcggtgtcg agcttccaca gccggaagtc
     3061 gcgctcgaag acgatgaggc ggccgtcgcg cgagatgctc ggccagagca cgcgcccgtc
     3121 gcggaagctc gtcacctgcc gggcctgttg gccgggcacg cgcgaccaga tgttttgcgc
     3181 gcccccacgg tcggagacgt agaagaggcg tcggccgtcc gatccccaca tcggccacga
     3241 ctgccgcgcg ccgcgctcgg ccacctgctc ataggtcgga gcggcgccag cgccgtcgag
     3301 accgcgcgcc acccagattt ccgactcgtc gatgtggctg ctgcccttgc gccaccactg
     3361 gcccgaactg atgccgcgcg ccgagaaggc taaggccgcg ccgtcaggcg agggggcgga
     3421 gaagaactcg ttggtgtagc ggtcggcgct cacctgcatc ggcgtgccgc ccgagggcga
     3481 gacgcggaag atgtcgttca tcccggcgat gtcgcgcgag gtcgaagaga aataaagcca
     3541 gcggccgtcg gcgctccacg cgtcgaggag gtcttgtccg tcgtcgaagg tgaggcgtcg
     3601 gagttcgccg gcgtcgaggc tgagcacgta gaggtcgccg ttgccggtgc gcgtggaggt
     3661 gaaggctagt tgacggccgt cgggcgagta cactgggcgg cgctcgtccg ccgggtgggc
     3721 gacgagcagg cgggccgcgc cgccgtcggc gggcgccgtc cagatgtcgc cgcccgagac
     3781 gaaggcgatc tccgagcggt cgggcgagag cgccggctcg gtgaagtaag gcgccgccgc
     3841 cacgacgacg gcgttacccg aatcttgtgc gaacgcgggc cgagccgcgg aggccgggaa
     3901 tagcgtcaag gcgagcgcga gcgccaacag gcgacgagtg cgaatcatgt gtcctccgaa
     3961 gatgagattt gccttctcag tagtatgaaa tcgtcctgcg tctgatgagc gtcaccgacg
     4021 cgggcgcgtg ctccgtgtgc cacgtcgtcc gccgctccgt cacccaactg ccccgcgcgt
     4081 cgtattcgta agcgtgagct tccctgtaaa tcagcgcgcc gcgcgggtcg taagcgtgta
     4141 cggtgacggt gtcgccgcgc gcgtcgtaag cgaagaccgt ctttgtgagc agcgcccggc
     4201 tctgctggtc gtaagagtag gtcgtgctcg ccgtgcgtcg ccccgaagcg tcgtacacgc
     4261 tcacgtactt gtcactcagc cgaccgcgct cgtcgccgct cgtggattcg gtcaggcgtc
     4321 cccccacgtc gtaggtgtag gcatagacta gaactgactt ctcgccggtg agtttaccgt
     4381 cctcgtcgaa gtgctcgatc tcccggcgca cgtcgttgcc gcgctcgtcg agcgtggtga
     4441 cttcgcgctt gcggagcctt ccgccgctgt cgtagctgtg cgactcgaca cgccgccccg
     4501 acgcttcgta gctatgcgta tcgcgtccaa agcggaggcc gcccaggtag agcgtcgtct
     4561 cgacgggccg gccccgcacg tcctgcctgt agacgaagcg cgccgcctcc ggctcatcgc
     4621 cgtcgaagct ctccgtcata aaccccttcg cgtcgtagtt gtcagtcttc gtgttgacgc
     4681 ggcgggagac ttcgcggccg tccttcatct cgacccgaaa ctcctccgtg cgaactgtct
     4741 tgacaggccc ctgaagcccc tgccacagct tgtcaggcac ggcgccccgc gcgtgcgccc
     4801 cctcctgcgc gcacaccgcg tgcgctctca gcagcgacac aacgacggca gcggcgagcg
     4861 ccgcgccgcg ccgacgccga cgacgcccgc gcgcccatac gggaaagtct ggtttcaact
     4921 cacacacttg atgggctaaa cttaagagcg agcctgcaaa caaaactgac ggaagaagcc
     4981 gccgaagtca acgggaatta tcgaccggcg gggcgcgtgt taacaagggg acggacgcgt
     5041 gacgctcgaa gagaagttga agaacctgcc cgtctcgccc ggcgtctatc tccacaagga
     5101 cgaggcgggc aagatcatct acgtcggcaa ggcgaagaac ctgcgcagcc gcgtccgctc
     5161 ctacttccag tcggggcgcg gtcacgaccg caagacgcgc gaactcgtcc gccgcgtccg
     5221 cgacctcgaa ttcatcgtca ccgacaacga ggtcgaagcg ttagtcctcg aatcgaatct
     5281 catcaagcag cacaagccgc gttacaacgt gctcttgaag gacgacaagc agtacccgca
     5341 cctcaagctc acgatcaacg agccgttccc ccgcgtgatg atcacgcgcc gcatccagcg
     5401 cgacggcgcg ctctacttcg gcccgttcct cccggcctct ttagcccgcc gcaccatcga
     5461 cctcatcaac cgcacgttcc agctccgcac ctgcgacatc gagattgacg gccgtctgcc
     5521 gcgcccctgt ctcgaatacc acatcaagcg ttgcctcggc ccgtgcgtga agggactgtg
     5581 tacgcccgag gagtacaagg agtcggtgcg cgacgtgcgg atgttcctcg aaggccgtaa
     5641 ccgcgagctg gccgacgagt atgaggggcg gatggcgcag gcgtcggacg agatgcgctt
     5701 cgagatggcg gccaagtatc gcgacctgcg gaaagtcgtc ctcgccgtct ccgagcagca
     5761 gaagatggcg acgacctccg agcgcgacgt ggacatcttc ggctactacc gcgaagggcc
     5821 gcgcctcgcc ctccaactct tcaccatgcg cgagggccgc atcgtcggcc gccgcgagtt
     5881 cttctgggaa gacctctcgg aagacgagtt cgacccggcg aagtttttgg gcgaagtgct
     5941 cacgcagtac tactcgaccg actacgtgcc gcgtgaggta cacgtcccct cagacttcga
     6001 cgaccgtgag ctgctggagc gcgcgctcac cgaccgaaaa ggtaggcgcg tccgcatcct
     6061 cgacccgcgc cgcggcgaga agagggacat gatcgagctg gtggagaaga acgccaagat
     6121 cgccttcgag cagcggttcc gggtgctgaa gcccgacatg gagcgcgtgc tcgaagagtt
     6181 gcaggagact ctggagctgc cgcgttttcc gtcgcgcatc gagtcgttcg acgtgtcgaa
     6241 catctcgggc gcggagaacg tggcctcgat ggtcgtgtgc gaaaacggca agatgaaccg
     6301 cgccgagtat cgcaagttca agatcaagac ggtcgaaggc gcaaacgacc cggcctcgat
     6361 gcgcgaggcc gtgctgcgcc gctaccgccg ccagctcgac gaaagaaacg cgctgcccga
     6421 cctcgtcatg atcgacggcg gcatcaccca gctcaacgcc tccgcctccg ccatgcgcga
     6481 gcttgacctc gaagccatcc ccgtcgtcgg cgtcgtcaag ccgccgcgcc gacacaacga
     6541 ggtctcccac ctgctcgtca agggccgcga gcacgaaccc gtttttatcg actcccactc
     6601 gcccgtcctg cgcctcatcc agatgatccg cgacgagacg caccgcacgg ccgtcgccta
     6661 ccaccgcaag cgccgcgagc tgcgcgactt cacctcggag ctgacggcca tccccggcgt
     6721 cggcgacaag cgcaagaacc gtctgctccg caacctcggc tccatccagc gcatcgcgca
     6781 ggccggcgtc gcggagctga cacccttcgt cggcgccaag acggccgccg agatcgtcga
     6841 acacttcaca cgccagcgcg cgcttgccga aagcggcg
//
LOCUS       DASVES010000139         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1212112, whole genome shotgun sequence.
ACCESSION   DASVES010000139 DASVES010000000
VERSION     DASVES010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212112"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /gene="feoB"
                     /locus_tag="VF240_05150"
     CDS             <1..1775
                     /gene="feoB"
                     /locus_tag="VF240_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX7174651.1"
                     /translation="PVEIDSARLAAALGVAVVPVTARQHRGLEELALAVLGAAAKRAP
                     GAGWRLSAHAEAEIELLGAGERAARAEAFADLYGDELPEDETRRAQVLAARARLAHEN
                     PDWWQEPLTARYEWIERVAASAVKKSSRPTARPRATERIDRVVTHRLFGPVVLLGVML
                     VVFQAIFSWASLPMELIDKGFGTLGESVRGALPEGLFTDLLVNGVISGVGGVLVFLPQ
                     ILLLFFFIALLEDSGYMARAAFILDRLMRGVGLHGKAFMPLLSSFACAIPGIMAARTI
                     ENPKDRLATILIAPFMSCSARLPVYTLMIAAFFSEDKVFGFVSVGALIILSMYLLGLA
                     VAVGVAWVLKHTILKSPPPPLVLELPPYRVPGLLNVAQATGTRGWLFVKRAGTVILAI
                     SILLWALVAFPRPDKAQTSETARAAVEQATRETGPESRGEAAPGVETRPADAAAEGEQ
                     VRNSYAGRLGRAIEPAIAPLGFDWKMGVGLISSFAARETLVSTLSIVYNVGKDAEADS
                     PSLVEAVRAAKRPNGRPAWTPLVAVSMMVFFVLACQCMSTVAVVRRETNSWRWPLFMV
                     GYMLVLAYGASFVTYQGGRLLGFD"
     gene            1784..2026
                     /locus_tag="VF240_05155"
     CDS             1784..2026
                     /locus_tag="VF240_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEX7174652.1"
                     /translation="MLDWQTIAVALIVLAALLYTARRALARLRSFSTSGRGGSASSCA
                     TGCGKCGDDEDAPAARPANTFVQISSERSRAPTKRA"
BASE COUNT          304 a          728 c          730 g          371 t
ORIGIN      
        1 ggccggtcga gatcgactcc gcgcgcctcg ccgcggcgct cggcgtggcg gtcgtcccgg
       61 tcacggcccg ccagcaccgc ggcctcgaag agcttgcact cgccgtgtta ggcgccgccg
      121 cgaagcgcgc ccccggcgcg ggctggcgcc tgtcagcgca tgccgaggcc gaaatcgaac
      181 ttctcggcgc gggcgaacgg gccgcgcgcg ccgaggcttt cgcagacctc tatggcgacg
      241 agttgcccga ggacgaaacg cgccgcgcgc aggttctcgc ggcgcgcgct cgtctggcgc
      301 acgagaaccc cgattggtgg caggagccgc tcacggcgcg ttacgaatgg atcgagcgcg
      361 tggcggcgag cgccgtcaaa aaaagttcgc gcccgacggc gcgtccgcgc gccaccgagc
      421 gcatcgaccg cgtcgtgacg cacaggctct tcggccccgt cgtcctgctg ggcgtgatgc
      481 tcgtcgtctt tcaggcgatc ttttcgtggg cgagcctgcc gatggagttg attgacaagg
      541 gcttcggcac gctcggcgag tcggtgcggg gcgcgctgcc ggaggggctg ttcacagacc
      601 tgctcgtcaa cggcgtgatc agcggggtgg gcggcgtgct cgtcttcctg ccgcagatac
      661 tgctgctctt cttcttcatc gcccttctgg aagacagcgg ctacatggcg cgcgccgcct
      721 tcattctgga caggctgatg cgcggcgtcg ggctgcacgg caaggcgttc atgccgctgc
      781 tttcgagctt cgcctgcgcc atccccggca tcatggcggc gcgcaccatc gagaatccca
      841 aagaccggct cgcgacgatc ctcatcgccc ccttcatgag ctgctcggcg cgtctgcccg
      901 tctacacgct gatgatcgcg gccttcttct cggaagacaa agttttcggc ttcgtctccg
      961 tcggcgcgct tatcatcctg tcgatgtacc tgttggggct ggccgtggcg gtcggcgtgg
     1021 cgtgggtgct caagcacacg atcctcaagt cgccgccgcc gccgctcgtc cttgagcttc
     1081 cgccctaccg cgtgcccggc ctcctcaacg tcgcgcaggc tacggggacg cgcgggtggc
     1141 tgttcgtgaa gcgcgccggc acggtcatcc tcgccatctc gattctgctg tgggcgctcg
     1201 tcgccttccc gcgccccgac aaggcgcaga cgagcgagac ggcgcgcgcg gccgtcgagc
     1261 aggcgacgcg cgagaccggc ccggagtcgc gcggggaggc agcgccgggc gtcgagacgc
     1321 ggccggccga cgcggcggcc gagggcgaac aggttcgcaa cagttacgcg gggcggttgg
     1381 gccgcgccat cgagccggct atcgcgccgc tcgggttcga ctggaagatg ggcgtcggac
     1441 tgatctcctc gttcgccgcg cgcgagacgc tggtctccac gctctcaatc gtctacaacg
     1501 tcggcaagga cgccgaggcc gactcgccct cactcgtcga ggccgtgcgc gcggcgaagc
     1561 ggccaaacgg gcgtccggcg tggacgcctc tggtcgccgt gtcgatgatg gtcttcttcg
     1621 tgctcgcctg tcagtgcatg tccacggtgg cggtggtgcg gcgcgagacg aattcctggc
     1681 gctggccgct cttcatggtc ggctacatgc tcgtgctcgc ctatggagcc tcgttcgtca
     1741 cttatcaggg cgggcggctt ctcgggttcg attgaggttg cggatgcttg actggcagac
     1801 catcgccgtc gcgctcatcg tgctcgccgc actcctctac acggcgcgcc gcgcactcgc
     1861 gcgcctgcgc tcattctcga cctcggggcg cggcggctcc gcatcgtcct gcgcgacagg
     1921 ttgcggcaag tgcggcgacg acgaagatgc gccggccgcg cggccggcga acacgttcgt
     1981 gcagatcagc tcagaacgta gtcgggctcc gaccaagcgc gcttagcatt caattcgaca
     2041 ccccaaagaa agtgtcaaca agccgcggag cggcgaaata atgtagccca cggcgtgagc
     2101 cgtgggtaaa gattgccaaa gaacaataaa gcc
//
LOCUS       DASVES010000140         8245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1581996, whole genome shotgun sequence.
ACCESSION   DASVES010000140 DASVES010000000
VERSION     DASVES010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581996"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VF240_05160"
     CDS             complement(<1..282)
                     /locus_tag="VF240_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174653.1"
                     /translation="MRTHRLLLPSLFLFALLSCLAPNVLANGGLNVSARDAVSEDAAL
                     RASALKALREAGPAGLEALFAEFADEIALRAGGQPAAGGAEWARIAEALD"
     gene            complement(463..3342)
                     /locus_tag="VF240_05165"
     CDS             complement(463..3342)
                     /locus_tag="VF240_05165"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7174654.1"
                     /translation="MRRERGSGGAEFVAEGDGIREYGLGENGMKVLLVENRVAPVVTF
                     CVLYRVGSRNEAVGYTGATHLLEHMMFKGTPDFNKERNTQIAATLQKIGADFNATTWY
                     DRTNYFETVPSDQLELAVRLEADRMRNSFIADADRQSEMTVVRNELERGQNEPMLVLD
                     EAVYAAAFREHPYHHPTIGWRADVENVPTARLKEFYDTFYHPNNATAIIVGDFDADAA
                     LDLVAKYFGAHPSAPGVIPEVYTEEPPQQGERRVVVRRAGELAFVQAAFHTPAVLGQT
                     PVLSNAELARRAASPPEANDIYPLVVLSAALSGGVTSRLYQALVETELAVNVTTNCDQ
                     FRDPGLFNVYATVRPGVESARVEDAIHAELARVAREGLSAEETQRARRQIVAQVAYNR
                     DGTSNVAIQISEAEAVADWRFYKDYAANIERVTPDDVRRVAEEYLTEDNRTVGHFIPK
                     TTNGNGNSAGDASAGGARSSRLKRLGGVRAARGPAHHHEPEAGESVTPQAAAQEAVAQ
                     RGEKSSDAARPNRPDADAGRTAGLAARVARTELETGATLLVLENRATPTVSVRGSLRA
                     GSFFEPRDRPGLARVTADMLERGTRRRTKLQLVGDLESAGADIEFSADPFAVNLSARS
                     LAQDLPLLIDTLAEQLREPSFPADELEKLKEQTVAAIQEQQSSTRHRAYERFSQLVYD
                     AENPFHLPPGERLVESIRSITVEDVRRFYEARYGGRSLVLSVVGDADAARVAEQFREA
                     FGAFAGPESVEVTVSDPQPREGSMREAVLLKDKANVDVLIGSATSLRRDVSDYYAALI
                     ANSALGESTLSSRLGLQVRDREGLTYGIGSRFRAPSLAAGPWYISVSVNPANVERAIE
                     SALTVLREYVREGLRPEELADEKSAAIGSFKVSLSTNTGLAEALWNAEFYRLGPDFLD
                     RYPELIQAVTLEEANTALRKYFRPDHLTVVVAGDTESAAPASVTT"
     gene            3603..4154
                     /locus_tag="VF240_05170"
     CDS             3603..4154
                     /locus_tag="VF240_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174655.1"
                     /translation="MDNLRFIRETMERATAFTAVPGWGQLIIGVTALFAALVASQQQT
                     GEAWLAVWFAEACLSLVIAGWAMSRKARAASTPLLSQPGRKLVINFAPPMFVGALLTI
                     VFWRAELAGLLPAVWLLLYGTGVVTGGAFSVRPVPVMGLCFMFLGAGALFCPAAWGDA
                     FMAVGFGGLHILFGLHIARYYGG"
     gene            4147..4554
                     /locus_tag="VF240_05175"
     CDS             4147..4554
                     /locus_tag="VF240_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7174656.1"
                     /translation="MARQSSLPKDSAPARTTRAARAGSIQPQRAAGGAGEMTPAELDR
                     VIHERMRLAIVSALAVNASLTFNDLKQMLNTTDGNLSVHARKLEEANYVVCNKFFDGR
                     MPKTEYKLTTSGRRALERYLNHMEAIIRKTRAG"
     gene            4669..5010
                     /locus_tag="VF240_05180"
     CDS             4669..5010
                     /locus_tag="VF240_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174657.1"
                     /translation="MKRIRIAPRRGRAPVALRLALLALPVVFWSAVFAVLAGVRPVQR
                     IVAATPDSAQVIVALACPLVAVLLGAAALRCEGRAGAGRGGGEARLGRLTVAAGMVLF
                     AFAVLASLGPA"
     gene            5372..7849
                     /locus_tag="VF240_05185"
     CDS             5372..7849
                     /locus_tag="VF240_05185"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEX7174658.1"
                     /translation="MTVGNGGAAATTMSDRTRLGLGVLEAALLLGLLGDALLRATPWG
                     LNVFLWVGALVAGAHALSRLGGARKLLTGEGGWLWPVALAFASFFVWRDSLTLRVLDA
                     LVILGALSVVALGARGGRVRLAGVTDYVAAAFVAGASAVFGVFPLLFADVNWKEIPRA
                     GWSRHALAATRGLLLALPLLLLFGALLMAADAVYEGLVRDTFAFDAELAFSHVFLLIF
                     FSWLAAGYLRALLLAKPPLTSAAAHAAGAALGISTAPNTTTTSGATKTSGASTNDNKA
                     ATGAPRTSVTDDAPAGGAGTTATAMPRGGYRFSSGDEEGADARAELKGEQPAAKEMAG
                     GAENKSDKSKTEASKPDDGKADANKADASKADVGQGGESNVPPRPAGGSPYASAPKSA
                     GVGVNPARSMRDVVSLGIVEVGVVLGLLDLLFFSFVAVQLGHFFGDAQHVVTSAGLTF
                     SDYARRGFFELVWVALLALPLLLAAHWLLRKENRAHERVFRVLAGAMVALLFVIMASG
                     LWRMRLYQQAYGQTELRLYTTAFMFWLGAVFVWFALTVLRGRRERFACGALVAWLVCL
                     GALHFVNPDDMIVRTNVAQAHALQRVERFDSEYAVSISADAVPALVELLPEMTARDRA
                     HVAARLTTWLNPAHTGDWRSWNYSRWRARQLVMENEVALREWEARWKAEAKLAEEEKM
                     RAKAQARAVTALGTIPGTRLSIRVERVPLVANQVRWFRRTAVLFSGDAVVSALSLAND
                     DGGPLARLEVFKVSDSLILLRDASGLYAADAERRTFIRSEAGALLGKATYVGAFDFDE
                     LKAWRFIPASEVARPPATGAAGGGGRM"
BASE COUNT         1211 a         2712 c         2911 g         1411 t
ORIGIN      
        1 gtctaacgcc tcggcgattc tcgcccactc cgcgccgccc gctgcgggtt gtccgcccgc
       61 acggagcgca atctcgtcag cgaactccgc aaacagtgct tcaagccctg cggggccggc
      121 ctcgcgcaac gccttgaggg ccgaggcgcg cagggccgcg tcctccgaga cggcgtcgcg
      181 cgccgagacg ttcagccccc cgttcgccag cacgtttggc gcgaggcagg acagcagcgc
      241 gaagagaaaa agggacggca atagcaggcg gtgggttcgc ataagaaaac ctctcttgag
      301 atgggaagtg acgcgtccgc gcgagagacg cccggagggc gtcttcacac gaatcattgt
      361 ggcgaggcgg tcggaagtgg gatgcgccgc cagcgcccgc gcgttggcgg cgcgcactcg
      421 cgtcgagtgg aaatattttc gggcgcgtcg tctgtgcctg tgtcaggtcg tgacggacgc
      481 aggggcggct gactccgtgt cgcccgcgac gacgacggtg aggtgatcgg ggcggaagta
      541 tttacgcagc gccgtgttgg cctcttcgag ggtgacggcc tggatgagtt cggggtagcg
      601 gtcgaggaag tcagggccga ggcggtagaa ctcggcgttc caaagggcct cggccagtcc
      661 cgtgttcgtc gagagcgaga ccttgaagga gccgatggcc gcggacttct cgtcggccaa
      721 ctcctcgggt cggagcccct cgcggacgta ctcgcgcagc accgtgaggg ccgactcgat
      781 ggcgcgctca acgttcgccg ggttgaccga gacggagatg taccacgggc cggcggccag
      841 agagggcgcg cggaagcgcg agccgatgcc gtaggtcagc ccctcgcggt cgcgcacctg
      901 tagcccgagg cgcgaggaga gcgtcgattc gccgagggcg ctgttggcga tcagggcggc
      961 gtaatagtcc gacacgtcgc ggcgcagcga cgtggccgag ccgattaaga catcgacgtt
     1021 ggccttgtcc ttcaggagca ctgcctcgcg catgctgccc tcgcgcggct gcgggtcgga
     1081 caccgtgacc tcgacgctct ccggccccgc gaacgcgccg aacgcctcgc ggaactgttc
     1141 ggcgacgcgg gcggcgtccg cgtcgccgac gaccgagaga acgagcgagc gcccgccgta
     1201 gcgcgcctca tagaagcggc gcacgtcctc gaccgtgatg gagcggatgc tctcgacaag
     1261 gcgctcgccc ggcggcaggt ggaaggggtt ctcggcatcg tagacgagtt gcgagaaacg
     1321 ctcgtaggcg cgatggcgcg tgctcgattg ctgctcctga atggcggcga ccgtctgctc
     1381 cttgagcttc tccaattcgt cggccgggaa cgatggctcg cgcaactgct cggcgagcgt
     1441 gtcgatgagc agcgggaggt cttgggcgag cgaacgggcg ctcaggttga cggcgaaggg
     1501 gtcggccgaa aattcgatgt cggcgccggc gctctccagg tcgccgacga gctgaagctt
     1561 ggtgcgccga cgtgtgccgc gctccaacat gtcggccgtg acgcgcgcaa ggcccggccg
     1621 gtcgcgcggc tcgaagaagg agccggcgcg cagggagccg cggacggaga cggtgggcgt
     1681 ggcgcggttt tcgagcacga ggagcgtggc gccggtctcc agctccgtgc gcgcgacgcg
     1741 cgcggcgagg cccgccgtgc ggccggcgtc ggcgtcgggc cggttcggcc gcgccgcgtc
     1801 ggaagacttc tcgccgcgct gcgccacggc ctcctgcgcc gcggcctgcg gcgtcacgga
     1861 ctcaccggcc tcgggttcgt gatggtgtgc cgggccgcgc gcggcgcgca cgcccccgag
     1921 tctcttgagt cgagaacttc tcgcgccacc cgccgacgcg tcgcccgcgc tgttaccgtt
     1981 gccgttcgtc gtcttcggga tgaagtggcc gaccgttcga ttgtcctcgg tcagatactc
     2041 ttcggccacg cggcgcacgt cgtcgggcgt gacgcgttca atgttcgccg cgtagtcctt
     2101 gtagaagcgc cagtcggcga cggcctcggc ctcggagatt tgaatggcga cgttcgacgt
     2161 gccgtcgcga ttgtaggcga cctgcgcgac gatctgccgg cgcgcccttt gcgtctcctc
     2221 cgcgctcagc ccctcacggg ccacgcgtgc aagctccgcg tgaatcgcgt cctcgacccg
     2281 cgcggattcg acaccgggcc tgacggtcgc gtagacgtta aagagaccgg ggtcgcggaa
     2341 ctggtcgcag ttggtcgtca cattcacggc cagctccgtt tcgacgagcg cctgatagag
     2401 ccgacttgtc acgccgccgg agagcgccgc cgagaggacg acgagcgggt agatgtcgtt
     2461 cgcctcgggc gggctggcgg cgcggcgcgc gagttccgcg ttcgagagaa caggggtttg
     2521 cccgagcacg gccggcgtgt ggaaggccgc ctgcacgaag gccagctcgc ccgcgcgtcg
     2581 caccacgacg cgccgctcac cctgctgcgg cggctcctct gtatagactt cggggataac
     2641 ccccggcgcc gaagggtgcg cgccgaagta cttcgcgacg aggtcgagcg ctgcgtccgc
     2701 gtcgaagtcg ccgacgatga tggcggtcgc gttgttcgga tgatagaacg tgtcgtagaa
     2761 ctccttgagg cgcgcggtgg gtacgttctc cacgtcggcg cgccagccga tggtcgggtg
     2821 atgatacggg tgctcgcgga aggcggcggc gtagacggcc tcgtctaaaa ccagcatcgg
     2881 ctcgttctgc ccgcgctcca gctcgttgcg caccacggtc atctccgact gccggtcggc
     2941 gtcggcgatg aaggagttac gcatgcggtc ggcttcgaga cgcacggcca gctccaactg
     3001 gtcggagggg acggtctcga agtagttggt gcggtcgtac caggtggtgg cgttgaagtc
     3061 ggcgccgatc ttctggagcg tcgcggcgat ctgcgtgttg cgctccttgt tgaagtcggg
     3121 cgtgcctttg aacatcatgt gttcgagcag gtgcgtggcg cccgtgtagc cgacggcctc
     3181 gttgcgcgac cccacccggt agaggacgca gaaggtgacg accggcgcca cccggttctc
     3241 gacgagcagg accttcatcc cgttctcacc cagcccgtac tcgcgtatcc cgtcgccctc
     3301 cgcgacgaac tcggccccac cgcttccgcg ctctctccgc acgctcaaat tgttccacct
     3361 ctggcgaatg aattgtaaag acgtgaggat ttgatgctaa caaatcgcgc cggggttgcg
     3421 aaagctgccg ggccaaaagc gggggcgccc ggcagacgcg cccgcgattg ctttgcattg
     3481 taaagtactt gacagaggtc ggccggcggg tgtattttgc gcgtatggct gtcattcgca
     3541 tgacgcgtga gcgagagccc tcggcggccg agggagaggc gccggccctt cacgaccggg
     3601 cgatggacaa tctgcgcttc atccgcgaga cgatggagcg cgccacggcc tttacggccg
     3661 tccccggctg gggacagctc atcatcggcg tcacagccct ctttgccgcg ctcgtcgcct
     3721 cacaacagca gacgggcgag gcgtggctcg ccgtctggtt cgccgaggcg tgtctgtcgc
     3781 tcgtcatcgc cggctgggcg atgagccgca aggcgcgcgc cgcgtccacc ccgctcctct
     3841 cacagcccgg tagaaagctc gtcatcaact tcgcgccgcc gatgttcgtc ggggcgctgc
     3901 tcaccatcgt cttctggcgc gcggagctgg cgggtctgct cccggccgta tggctcctgc
     3961 tctacgggac gggcgttgtc acgggcggag ccttctccgt cagacccgtc ccggtgatgg
     4021 ggctgtgctt catgttcctc ggcgccggcg cgctcttctg ccccgcagcc tggggcgacg
     4081 ccttcatggc cgtcggcttt ggcggcctcc acatcctgtt cggcttacac atcgcgaggt
     4141 attacggtgg ctagacaaag ttccttaccg aaagatagcg cgccggcgcg gacgactcgc
     4201 gccgcgcgcg ccggcagcat tcagccgcaa cgggcggcgg gcggcgcggg cgagatgacg
     4261 cccgccgaac tcgaccgcgt catccacgag cgcatgcgcc tcgccatcgt cagcgccctc
     4321 gccgtcaacg cctccctcac cttcaacgac ctcaagcaga tgctcaacac gacggacggc
     4381 aatctgagcg tccacgcgcg caagctcgaa gaggccaact acgtcgtctg caacaaattc
     4441 ttcgacggcc ggatgccgaa gaccgagtac aagctgacga cctcggggcg gcgggcgctc
     4501 gaacgctacc tcaatcacat ggaggcgatc attagaaaaa cgcgtgcggg gtgaggcccc
     4561 gaagcgcgaa tttttttgcc catctacttt gaatggcaaa gggctttgca tcaagtcgcc
     4621 gtggggcgcg ggggctggcg gcacgggtta tgaaaggagt gacggaagat gaaacgcata
     4681 cgaatcgcac cgaggcgcgg gcgcgcgccc gtcgcgctga ggctcgcgct actcgcgctg
     4741 ccggtcgtct tctggtcggc cgtcttcgcg gtgctggcgg gcgtgcggcc cgtccagagg
     4801 atcgtcgcgg ccacgcctga ctcggcacag gtcatcgtcg cgctcgcctg cccgctcgtc
     4861 gccgtgctcc tgggcgcggc ggcgctcagg tgtgagggcc gcgcgggcgc tggaaggggc
     4921 ggtggcgaag cgcgtctggg ccgcctgacg gtcgcggccg gcatggtgct cttcgccttc
     4981 gccgtgctgg cgtcgctcgg cccggcgtga cgggcgaacg ggcgatgaac cactgaccat
     5041 tagctgttag ctgttagctg ttagccattg accacggaca aaggacaact gagctgtcct
     5101 ctcgcatgcg aaaggggaac cggacgtgaa tacgaacgtg aatctggaga cagcattggg
     5161 cgtactggcg ctcatggacg gctgattggg cgcgcggcgc gagacgcacg cgcccggccg
     5221 cgtgccccgg ccatgtggat gcccacaacg ggggcacgga gttccacgca ttgccgctaa
     5281 ggtcatgtgc gtccatcctc ggcaccactg gtgtgaaaga aaaacttcgc gcggcgcgcg
     5341 ggcgcgttga ctttggaaag gggcgtttga gatgactgtg gggaatggcg gtgcagcggc
     5401 gacgacgatg agcgaccgga cgaggctcgg gctgggcgtg ctggaggcgg cgctgttgct
     5461 ggggctgctc ggcgacgcgc tgctgcgcgc gacgccctgg gggctgaacg tttttctgtg
     5521 ggtgggcgcg ctcgtggccg gggcgcacgc gctctcgcgc cttgggggcg cgcgcaagtt
     5581 gctcacgggc gagggcggct ggctgtggcc cgtggcgctc gcgttcgcgt cctttttcgt
     5641 ctggcgcgac tcgctgacgc tgcgcgtgct cgacgcactc gtcatcctcg gcgcgctctc
     5701 ggtagtcgcg ctcggggcgc gcggcgggcg ggtgcggctc gcgggcgtga cggactacgt
     5761 ggcggccgcc ttcgtcgcgg gcgcgagcgc cgtgttcggc gtcttcccgc tcctgttcgc
     5821 ggacgtgaac tggaaggaga ttccgcgcgc ggggtggtcg cgccacgcgc tggcggcgac
     5881 gcgcgggctg cttttggcgc tgccgcttct gctcctcttc ggcgcgcttc tcatggcggc
     5941 cgacgccgtc tacgagggcc tcgtccgcga caccttcgcc ttcgacgcgg agttagcctt
     6001 ctcgcacgtc ttcctcctca tcttcttctc gtggctcgcg gccggctacc tgcgcgccct
     6061 gctgctcgcc aaacctccgc tcacctccgc ggcggcccac gcggcaggcg cggcgctcgg
     6121 catctcaacc gcgccgaaca cgacgacgac gagcggcgcg acgaagacca gcggcgccag
     6181 cacaaacgac aacaaagccg cgacgggcgc gccccgcacg tccgtgaccg acgacgcgcc
     6241 ggcgggaggc gcgggaacga cggcgacggc gatgccgcgc ggcggctacc gcttctcttc
     6301 gggtgacgag gaaggcgccg acgcgcgcgc cgagttgaag ggcgagcagc cggcggcgaa
     6361 agagatggcc ggcggcgcgg agaacaagtc ggacaaaagt aagaccgaag cgagtaagcc
     6421 tgacgatggt aaggccgatg cgaacaaggc ggacgcgagt aaggccgacg ttggtcaggg
     6481 tggcgaaagc aatgtgccgc cgcggcccgc gggcgggagt ccttatgcgt ctgccccgaa
     6541 gtccgcaggc gtcggcgtca atccggcacg ttccatgcgt gatgtcgttt cgctcgggat
     6601 cgtcgaggtc ggcgtcgtgc tcggtttgtt agacctgctc ttcttcagct tcgtggccgt
     6661 gcagctcggc cacttcttcg gcgacgcgca gcacgtcgtc acctcggcgg gtttgacctt
     6721 ctccgactat gcgcgacgcg gcttcttcga gctggtctgg gtggcgctgc tggcgctccc
     6781 gctgctcctc gccgcgcact ggcttctgcg caaagagaac cgcgcgcacg agcgcgtctt
     6841 ccgcgtgctg gcgggggcga tggtcgctct gctgttcgtc atcatggcct cgggcctgtg
     6901 gcgcatgcgc ctctaccagc aggcttacgg gcagacggag ctgcgtctct acacgacggc
     6961 cttcatgttc tggctcggcg cggtcttcgt ctggttcgcg ctcaccgtgc tgcgcggccg
     7021 gcgcgagcgt ttcgcctgcg gcgcactcgt cgcgtggctc gtctgtctcg gcgcgctcca
     7081 cttcgtcaac cccgacgaca tgatcgtccg cacgaacgtc gcgcaggccc acgccctcca
     7141 gcgcgtcgaa cgcttcgact ccgagtatgc cgtctcgatc agcgccgacg ccgtgcccgc
     7201 gctcgtcgaa ctcctgcccg agatgaccgc gcgcgaccgc gctcacgtcg ccgcgcgcct
     7261 caccacgtgg ctcaacccgg cgcacaccgg cgactggcgc tcctggaact attcgcgctg
     7321 gcgggcgcgg cagttagtca tggagaacga ggtggccttg cgcgaatggg aggcgcgctg
     7381 gaaggccgag gcgaagttgg cggaggagga gaagatgcgg gcgaaggcgc aggcgcgggc
     7441 ggtgacggcg ctggggacga ttcccggcac gcgcctctcg attcgcgtcg agcgcgtgcc
     7501 cctcgtcgcc aaccaagtcc gctggttccg gcggacggcc gtactcttct ccggcgacgc
     7561 ggtcgtctcc gccctcagtc tggcgaacga cgacggcggc ccgctcgcgc gcctcgaagt
     7621 gttcaaggtc tccgactcgc tcatcctctt gcgggacgcg agcggcctct atgcggccga
     7681 cgccgagagg cgcacattca tcagaagtga agcgggcgcg ctgctcggca aagcgaccta
     7741 cgtcggcgcg ttcgacttcg acgagctgaa ggcgtggcgc ttcatcccgg cgtcggaggt
     7801 ggcccggcct ccggcgaccg gagccgccgg aggcggcggc agaatgtagc ccacggcgtc
     7861 agccgtgggt tcagtgtcga ttgcaaacat ggaagccccg gaggggcgaa agaatctcag
     7921 ggtgggacag cgtgcccgcc gattttcttt cgcccctccg gggctgcctt acttttatcc
     7981 cgtgtgttca cacggctcac gccgtgggct acattctgcc gccgctccgc ggctcattga
     8041 tgcttgcgat tgctctagct acggactcct tgtttacctg cgccgcgttg ccggccgcga
     8101 ctcactgccg gaaccttccc tgatattcgc ccgagactaa gaacgacttg accatctcgg
     8161 ccgcgacgaa gttgccgccg tggttgttga gcttcgcgag ccagaagttg aagcccgcga
     8221 ggtcgagcgt cgcctcgggc gggtc
//
LOCUS       DASVES010000141        15473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_788417, whole genome shotgun sequence.
ACCESSION   DASVES010000141 DASVES010000000
VERSION     DASVES010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15473
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788417"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VF240_05190"
     CDS             complement(<1..814)
                     /locus_tag="VF240_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174659.1"
                     /translation="MSDYLLSHLRARLARSLYVCVAALWACAGAFAQSASTAAPVLLT
                     EGTGATTRAVAYESVSRSGEPFRVTAPVAWEPDSRTRVMLFAMNLSLLPGEGASALTA
                     DAEDAAGRRYPLKVEYVGKPPYTEFDVPNKAYRDVGQGWLFAIVLRLNDEMADDLGDV
                     LVRINLHGVSSNRVRIRIGQTGGGPPTDAVAEVISPAPAATPTPMPALQPKTYGPNET
                     ADADAVRLLEQASWGPTEAEVARVKSIGFRQYVNEQLGAPATNYVDMLFPNDQ"
     gene            complement(1137..1313)
                     /locus_tag="VF240_05195"
     CDS             complement(1137..1313)
                     /locus_tag="VF240_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174660.1"
                     /translation="MTDNRDENQQQDRQKAATAGDGSESESKAQTGDPGRTPGKAEGD
                     EETIDEALRRKENG"
     gene            complement(1508..2296)
                     /locus_tag="VF240_05200"
     CDS             complement(1508..2296)
                     /locus_tag="VF240_05200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7174661.1"
                     /translation="MAAREEALEGAGALGTPARSEGQRSFVNVGQAERWASAIGGGAL
                     ALYGLTRGSMGGVALALIGGSLIYRGASGHCTVYSAAGIDTSGAEGDNPNVSVRGGHG
                     TKVEKSVTINRPPAELYSFWRNFENLPRFMNHLESVKVSGNDRSHWVAKGPAGTSVEW
                     DAEVYNEKENELIAWRSLEGSEVANAGSVHFEKAAGGGRGTVVRVVLKYDPPAGKLGA
                     AVAWLFGENPSQQIDEDLRRFKQLMETGETATTEGQTSGRAAKA"
     gene            2830..3807
                     /locus_tag="VF240_05205"
     CDS             2830..3807
                     /locus_tag="VF240_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEX7174662.1"
                     /translation="MSNFKPDLLTILISCGLSVAGVPTCAEAAKAATQSNNAANNQAA
                     QQSGSAEIVRESPRGRQGGMKVLAEGSYGQVSDPFIAVTRDAQVYAALRQWVKELPPL
                     ADSFFKSHAVVAVFTGLHNTGGYGIEMTRSGRGQLLVSESVPPPDMMTTQALTKPFKV
                     VAVTAKEDETVSVVLQGALAAAVLRPYRVASGEFTPDAGGAARAKTSGLEGELKVARY
                     EGLATVFFDLRGVGAKGSNFLQTSATAVVGYDGLFSLTSLEANTLAGRASRRLRVTGR
                     FTGVDEDKLDLDFALPSSKVAEGLSRVGKLAAVATAPAPPKRQPGASMY"
     gene            complement(3936..9158)
                     /locus_tag="VF240_05210"
     CDS             complement(3936..9158)
                     /locus_tag="VF240_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7174663.1"
                     /translation="MDEPTQVQEASSAEIAAATNSDGQVAVLVELTDAPATTVYADKL
                     ESSPLPRDAAMTEATNAARAQLTKITRAQETLAATLLGGKYDATEIYRVQRVLNGIAV
                     SVPPGQLDALRQLPGVKAVHLLVLEYPTNSTSVPFVGAPELWNNSAGAPGGPVMGAGI
                     KVGIIDTGIDYQHSNFGGRGLLADYTANNRTQNVDGFFPTQKVAGGFDFAGDAYNGSA
                     ATAAPDPDPMDCNGHGSHVAGTAAGLGVNADGTTFTGPYGTNTPFNSLRIGPGVAPRA
                     SLYALRVFGCNGGTNLTVAAIEWAIDPNGDGDFEDRLDVINMSLGSNFGVPSNPSALA
                     SDNAVRAGVIVVASAGNAGDTFFISGAPGVSGRAISVASVLDPGVGVNRSRINSPPAI
                     VRDLTVGNALFGPQLFDVTGDVVVGIDVAEPADPGLGLAAGTTTDGCSPLTNAAAVVG
                     KIVLVDRGRCAFEQKAKNAQDAGAIGTMIANNVAGVSNLSDDPTITSSNTIPTVLIQQ
                     ADANAIKASITGGNTVNVSLRRIPGSDTISSFSSRGPRGGGTSAIFALKPDISAPGQD
                     ITSVQTGVTCLTAQAAGCTGVASASGFQPNNQTLTISGTSMAAPHVAGAMALLRELNP
                     TWTVEELKAQVMNGAIRDVFTDVGGAGTRRGPGQIGAGRLDVPSAARTQVVAYNALDH
                     GLVSVSFDNEVVQGATRIKPVRVVNKGTTQITYTLEIDTLSDAPGVSYSLPQGTTITV
                     EAGQTFVFPVRMDAVEDQMDHTRDPSIPATQTGASPFNVVFANFPRHYLTEEGAYINF
                     KIAGATRIRLPVYSPARPTSTMTATDSIPAGASSIALNGTDVCTGTRAAGPTCTGSFP
                     VDVVSLVSPFELQVVSPRDTDISGEQDIRNVGVAYDATTNRIRFGVASYEEWSTPNDV
                     AYSIFIDADENGTYERVVVNTSPGAIARRNFGNANASAQDIFITLVFTPPGSVAFNSG
                     WLLNAASAAQFDTVVFNNNVMFLSVDPAAIGLSAGNTNFRYKVETCPGFAPLCSSLEG
                     FSPSDRAAGPYTWDYANPGLDFGNQVLVQDLNGKTIPVTANLTNLTNNGSLGALLLHH
                     HNARGTRAEVVAVDGLATAAVADLGLSLTVNNAAPNAGSTITFTLSVTNNGPAAATNV
                     RVTSTELPIGLTYLSDNSGGTFDPSTRTWTVGNLASGATATLNVVTAVSSGESPGAIT
                     ARIASFEQVDVNPANDQATVVFNAANAADLRLTLESDVLATVSGGEVAYTLVLTNNGE
                     DTALEIVVTGVFTPSSIAFSDASVTQGVFYPATGVWRIASLGKGVSARLTYTATTPSN
                     VCNQFSSSATVTAETFDPVPSNNSVTATTRINDTPCVALSAATYTVSEGAGFVDVTVN
                     RTGATTGPATVNYATTDGSANQKGDYIIALGTLEFAAGEQSKTIRIFITDDTYVEGDE
                     NFTLTLTDPSTGSLTTSPSSAVITITDNDTNAATNPIDNVEFFVRQHYRDFLNRDPDA
                     AGFAFWVDNLNSRLAACGGLVGVQLSRCRTGAYARVSEAFFLSVEFQQTGFLVYRFYK
                     ASFPETASRPRALPRYTEFMRDTQEIGEGVVVGSPGWEAALAANQEAFALEFVQRAEF
                     TTRYPTTQTPAEFVDALYATAGVTPSAAERQAAIDEFAAAPTSADAAARARVLRRVAD
                     NSQFSQREFNPAFVLMEYFGYLRRNPDDAPDNNFNGYDFWLSKLNSFTDFRQAEMVEA
                     FISSGEYRQRFGPN"
     gene            complement(10044..10958)
                     /locus_tag="VF240_05215"
     CDS             complement(10044..10958)
                     /locus_tag="VF240_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7174664.1"
                     /translation="MRVAVVGLGDIARKAYLPVLAARGDVELIFCTRDRETLGRLSRA
                     YRVSEVTTDYRELPGRGVEAAFVHTSTESHAEVASELLRGGLHVHLDKPISYTYEESV
                     AVVEAAERAGRVLAVGFNRRFAPMYAGLKAKPGRRLVLMQKNRAYLPEHPRRFVFDDF
                     IHVADTLRFLCPAEALAERVTFFTQEGLLRHLTLHLEGDGFAAFGVMNRDSGADEEVL
                     EVMSPGNKWDVRGLDVTTHYTGGEERVRRFRNWDTVLHRRGFPQMVEHFLELAGGRGD
                     APSALRDALQTHALCERIVARIEGGQRL"
     gene            11364..12191
                     /locus_tag="VF240_05220"
     CDS             11364..12191
                     /locus_tag="VF240_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7174665.1"
                     /translation="MNDDYDETSGPELTADEEALLKEMPTLTDVGQARRNFLGQTIAG
                     GLGAFALMLLEKEQALAALATAPDAAFAPAASLENPLRVVLKVNGAAKTLEVDSRMVL
                     LDALRERLELTGTKKGCDQGQCGACTVLVDGRRVLSCLTLAATVEGREVTTVEGLARG
                     DELHPVQTAFIKYDGFQCGYCTPGQICSAVGLLEEARRGEVSHVTEDVTVTTTRLTDD
                     EIRERMSGNICRCGAYPGIVAAIREAHSGRETARTWRFVEEEELARVRKEWEADETV"
     gene            12178..13167
                     /locus_tag="VF240_05225"
     CDS             12178..13167
                     /locus_tag="VF240_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX7174666.1"
                     /translation="MRPFKYTRASNPAAAARAVALNPRAKFLAGGTNILDLMKEDVER
                     PNELVDLTRLGLTEIKAARDGGISIGALAKNTDTANHPLIRQNYPLLTQAIVAGASGQ
                     LRNMATNGGNLMQRTRCQYFYDTAMPCNKREPGTGCGALEGLNRIHAILGWSDKCVAT
                     YPGDMANALSALDAVVRVRGAGGRERTIPIGEFHRLPGDTPERDNNLQHGELIVSIDL
                     PKSNFAKNSYYLKVRDRASYAFALVAVAAALEMNGGRISQARVVLGGVAHKPWRSPEA
                     EAALSGRPASEESFRQAAEAALKDAKPLAHNAYKVELGKRAIVRSLMRASRLA"
     gene            13209..15425
                     /locus_tag="VF240_05230"
     CDS             13209..15425
                     /locus_tag="VF240_05230"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX7174667.1"
                     /translation="MPRYIGKEMSRVDGVAKVTGRAKYAAEFRAPNLAYGFIVLGSVA
                     KGTIKSIDTREAESATGVVRVFTHLNAPKLGPKASTEQAPPRATEEQDKSFRALQSDR
                     IFFNAQPVALVVAETYEQARYAARLVKVSYNAEKHETDTEAVRGRARVPSQGPPPKPR
                     GNPEEAMKRAPVKVEAEYRIPIEHHNPMEPHAAVAVWQGDRLTVFDKTQGVYGVREHL
                     ASSFGVPEKNISVISPYVGGAFGSSLRPNYYPALTAMAAREIKRPVKVVYTRTQMFTG
                     HGYRPYTIQKIALGAEASGKLSAMIHEATHNTSALEEFSDDTTRFTRQVYACPNLYAP
                     LKITDTDLPTPTWMRAPGAVSGMFALECAMDELAYALKIDPLELRLINYAEVDPESGK
                     PFSSKALRECYRLGAEKFGWKERKFEPRSMRDGRLLVGWGMATGVWGAFQMPATARIT
                     FKADGTAHVASATSDIGPGTYTVVTMIAAEYLGLKPEQVKFELGDSNLPRAPSQGGSW
                     TTASVGSAVHGAALAVGARLLALANGEANSPLKGVAVADVEMLDGRLRLKSDPSRFVS
                     VSEVMKRNGLAEIIETFEARPSKEREKYATLAHGAQFVEVKIDPDLGNVRVTRVIEVT
                     ACGKIINPKASHSQEIGGVVWGIGMALQEATEIDHRYGRIMNPNLQHYHVPVNADIHM
                     IETMFVEEDDKIVNPLGVKGMGELGMVGIPAAIANAVFHATGRRIRDLPITPDKLL"
BASE COUNT         2740 a         4837 c         5157 g         2739 t
ORIGIN      
        1 actgatcgtt ggggaacagc atgtctacgt aattagtcgc gggcgcgccg agctgctcgt
       61 tgacgtactg ccggaagccg attgatttga cgcgcgccac ctccgcctcg gtcggccccc
      121 acgacgcctg ttcaagtaga cgaacggcgt cagcgtcggc cgtctcattc ggcccgtaag
      181 tcttcggctg gagcgcgggc atcggcgtcg gcgtcgccgc cggggcgggc gaaatcactt
      241 cggccactgc gtcagtcggc ggcccgccgc ctgtctgacc gatccggatg cggacgcggt
      301 tcgacgagac gccgtgaagg ttgatgcgga cgagcacgtc gccgaggtcg tcggccatct
      361 cgtcgttgag gcgcaggacg atggcgaaga gccatccctg ccccacgtcg cgatacgcct
      421 tgttcggcac gtcgaactcg gtgtaaggcg gcttgccgac gtactcgacc ttgagcgggt
      481 agcgacgacc ggccgcgtcc tccgcgtcag ccgtcagcgc gctcgcgccc tcgcccggca
      541 gcaggctgag gttcatggcg aagagcatca cgcgtgttcg agaatccggc tcccaggcga
      601 cgggcgcggt cacacgaaat ggctcgccgc tgcgcgagac ggactcgtag gccacggcgc
      661 gcgtcgttgc gcccgtcccc tcggtgagca gcacgggagc ggccgtcgag gctgattggg
      721 cgaaggctcc ggcgcaagcc cagagtgcgg cgacgcacac gtagagtgag cgtgcgagcc
      781 gcgcgcggag atgggagagc agataatcag acatatgaca ccagactcag gagtcttcca
      841 tcaaatgagg cggggggcga aactgttcgg gtgaaaactg aggtcgaaag gacttccctt
      901 tcgcggtcca tgcacacaaa atataccaca tccgcagacc gtggaaaacg acttcctttt
      961 cgccgccgcg cggccatgca agggcttctc ctacaaacgg gaagccttcc ggtgctgcgc
     1021 aggtttagac ggggcggggc ggcgaattgt cggaattttt tcttgaggcg ggtatgcgga
     1081 gacggccggc atgtctacac acgccggccg tctttatgaa gcctgaagat tcggcctcag
     1141 ccgttctctt tccggcgcag tgcttcgtcg attgtctcct cgtcgccctc ggccttgccc
     1201 ggcgtgcggc cggggtcgcc cgtctgcgct ttggactcgc tctcggagcc gtcgccggcc
     1261 gtggccgcct tttgccggtc ttgctgttgg ttctcgtctc ggttgtctgt catgattttc
     1321 ctctcttgaa ttgcttacgg cttcatcacg accttgatgc agtttagaca gtggccagtt
     1381 gtttgtgacg gtctgctcgc cgctgacact gaaaggttca agccgtgaga cgaaaacaac
     1441 tggccactga ccacggacaa agacaactct tagctaattg ctaaccgcta atagctaacc
     1501 gcttccctca cgccttcgcc gcgcgccccg aggtctggcc ttcggtggtc gctgtctcgc
     1561 cggtctccat gagctgcttg aagcggcgca ggtcttcgtc gatctgctgc gagggattct
     1621 cgccaaagag ccacgcgacg gccgcgccga gcttgccggc cggcgggtcg tacttgagca
     1681 cgacgcggac gaccgtcccc cgccctccgc ccgccgcctt ctcgaagtgg acggagccgg
     1741 cgttcgcgac ctccgagcct tcgagcgagc gccacgcgat cagctcgttc tccttctcgt
     1801 tgtagacttc ggcgtcccac tcgacgctcg tccccgccgg ccccttcgcc acccagtgtg
     1861 agcgatcatt acccgacacc ttcacggatt cgaggtggtt cataaagcgc ggcaggttct
     1921 cgaagttgcg ccagaagctg tacagctccg cgggcggacg gttgatggtt acgctcttct
     1981 caaccttagt gccgtgcccg ccccgcacgc tcacgttcgg gttgtcgccc tccgcccccg
     2041 aagtgtcgat gccggccgca ctgtagacgg tgcagtgacc cgacgcgcct ctatagatca
     2101 gcgacccgcc gatgagcgcg agcgccacgc cgcccatcga cccacgcgtc agcccgtaga
     2161 gcgcgagcgc gcccccgccg atagccgacg cccagcgctc cgcctgaccg acgttcacga
     2221 aagacctctg cccctctgac ctcgccggcg tccccaacgc gcccgcgcct tccaatgctt
     2281 cttccctcgc tgccatcttc gtatcctccc tttaaaatga tgatttcaga acacgtctcc
     2341 gtgacagaca cacgtccgcc tgtccgaaaa cggctcgcca taagtgtgtt gagaacatga
     2401 gtaacaaaac tcgtgccgtt tcgaccccgt cggggagcgg cgggaatgtc cgcatacgca
     2461 cactgacatt agacacacac gccaatccac atcgcgcccg tacacccgcg cgaaaaaaaa
     2521 gaaggcgggc gacactcttc ggtgcgcccg cctccctctc atcccctggc tcgttccgtg
     2581 aagcgtcggc ctgagcttta atgcttcact aatgatagcc ggtgtaccga ctgccagaga
     2641 ttgtaacgaa ggcgggccgc ggcgtctgta gttaaaccga cacgtaacct tgataaatgt
     2701 ttgtctcccg cgacggccct ctcctccggc gtgcaactcc gcgacctgaa cgttattcta
     2761 acgatgcgcg cgtaatcgcg cgcggcgtga ccgacgcatc ttagtcgtga tgttggagag
     2821 tgggccggag tgtcaaactt taaacctgac ctgttgacga ttcttatctc ctgcggcctg
     2881 agcgtcgcgg gcgtgccgac ctgcgcggag gcggcgaagg ccgcgacgca gagcaacaac
     2941 gcggcgaaca atcaagcggc tcagcaatcc ggctcggcgg agatcgttcg cgagtccccg
     3001 cgggggaggc agggcggaat gaaagttctc gccgaaggca gttacggtca ggtcagcgac
     3061 cctttcatcg cggtcacgcg cgacgcgcag gtttacgcgg cgttgcgcca gtgggtcaag
     3121 gagctgcccc cgttagccga tagcttcttc aaatcgcacg cggtggtcgc cgtcttcacc
     3181 ggattacaca acacgggcgg ctatggcatc gagatgacgc gctccggccg ggggcaactg
     3241 ctcgtgtccg aaagcgtgcc gccgcccgac atgatgacga cgcaagcctt gacgaaacct
     3301 ttcaaggtcg tggccgtgac agcgaaagag gacgagactg tctccgtggt gttgcagggc
     3361 gctttggcgg cggccgtcct gcgtccatac cgcgtcgcct cgggcgagtt cacaccggac
     3421 gccggcggcg ccgcgcgcgc gaaaacgtcg ggactcgaag gcgaactgaa ggtggcgcgt
     3481 tacgagggat tggcgacagt gtttttcgac ctcagaggtg tcggcgcgaa gggatcaaac
     3541 tttttgcaga cctccgcgac ggccgtcgtg ggatacgacg gcctgttctc gctgacgagc
     3601 cttgaggcca acacgttagc cgggcgggct tcgcgccgcc tacgcgtcac gggtcggttc
     3661 acgggcgttg atgaagacaa gctcgatctc gacttcgcat tgccgtcgtc gaaggtagct
     3721 gaggggttga gcagagtggg gaaactcgcc gcggtagcta cggcccccgc gccccccaaa
     3781 agacaaccgg gcgcgagcat gtactgagca gtaattgaaa tctgtaaggc gaaagccgcc
     3841 aggtgtcaga ggacatctgg cggctttcgc gttgggtagc agtctggtgg tcgtcggcgc
     3901 gtcggctgct gttcgacgcg cccacatttc gttctctagt tcgggccgaa gcgctggcgg
     3961 tactcgccgg agctgatgaa ggcttcgacc atttcagctt gccggaagtc cgtgaaggag
     4021 ttgagcttgc tcagccagaa gtcgtacccg ttgaagttgt tgtcgggagc atcatcgggg
     4081 ttgcgacgca agtagccgaa gtactccatc aacacgaacg cggggttgaa ctccctctga
     4141 ctgaactgcg agttgtcggc cacgcggcgc aggacgcgcg cgcgggctgc cgcgtcggcg
     4201 ctggtcggcg ccgcggcaaa ttcgtcaatc gccgcctgac gttccgccgc cgacggagtg
     4261 accccggcgg tggcgtagag cgcatcgacg aactcggcag gcgtctgcgt cgtcgggtaa
     4321 cgggtggtga attccgcacg ttggacgaac tccagtgcga aagcctcctg attggccgcg
     4381 agcgcggcct cccaacccgg cgagccgacg acgacgccct cgcctatctc ctgcgtgtcg
     4441 cgcatgaatt ccgtataacg cggcaacgcg cgcggccgcg acgccgtttc agggaacgac
     4501 gccttgtaga accggtagac gaggaagccc gtctgctgga actcgaccga gaggaagaag
     4561 gcttcggaca ctctggcgta ggcgccggtt cggcacctcg acaactgcac gccgaccagc
     4621 ccgccgcagg cggcgagacg gctgttgagg ttgtccaccc agaaggcgaa gcccgccgcg
     4681 tcagggtcac gattgaggaa gtcgcgatag tgctgacgga cgaagaactc gacgttgtca
     4741 atcgggttcg tcgccgcatt ggtatcgtta tccgtgatcg tgatgacggc cgagctgggg
     4801 ctggtggtca ggcttcccgt tgacgggtcg gtaagcgtca gggtgaaatt ctcatcgcct
     4861 tcgacgtaag tgtcgtcggt gatgaaaatc ctgatggtct tgctctgctc gccggcggcg
     4921 aattcgagcg tgccgagggc gatgatgtag tcgccctttt gattggccga gccgtcggtg
     4981 gtcgcgtagt tgacggtggc cggccccgtg gtggcgccgg tacgattcac ggtgacgtca
     5041 acgaagccgg cgccctcgct gaccgtgtag gtggccgcgc tcagtgcgac gcacggcgtg
     5101 tcgttgatcc tcgtcgtcgc cgtcaccgag ttgttgctcg gcacgggatc gaaggtctcg
     5161 gccgtcaccg tcgcgctgct gctgaactgg ttgcagacgt tgctcggcgt cgtcgccgtg
     5221 taggtgagtc gcgccgagac gcccttgccg aggctggcaa tcctccagac gcccgtggcc
     5281 gggtagaaga cgccctgcgt caccgaggcg tcactgaagg ctatcgacga aggcgtgaag
     5341 actccggtga ccacgatttc gagggcggtg tcctcaccgt tgttggtcag gacgagcgtg
     5401 taagcaactt cacctccgct gaccgtggcc agcacgtccg actccagggt gagacgaagg
     5461 tcggccgcgt tcgccgcgtt gaacacgacg gtcgcctggt cgttcgccgg gttcacgtca
     5521 acctgctcga acgacgcgat gcgcgcggtg atcgcccccg gagactcgcc cgaactgacg
     5581 gccgtgacga cattcaaagt ggccgtcgcg ccgctggcga gattgcccac cgtccacgtt
     5641 ctcgtggacg gatcgaacgt cccgcccgaa ttgtccgaga ggtacgtcag cccgatgggc
     5701 aattccgtgg aagtgaccct cacgttggtc gcggccgccg ggccgttatt tgtcacgctc
     5761 agcgtgaagg tgatggtcga gcccgcgttc ggggcggcgt tgttcaccgt cagactgagg
     5821 ccgaggtcgg ccaccgccgc cgtcgctagt ccatcgaccg ccacgacttc ggcgcgggtg
     5881 ccgcgcgcgt tgtggtggtg aaggagcagc gcgccgagcg agccgttgtt ggtcaggttg
     5941 gtcaggttgg ccgtgaccgg gatggtcttg ccgttgaggt cctgaacgag cacttgattg
     6001 ccgaagtcca gaccggggtt ggcgtagtcc cacgtgtaag gcccggccgc gcggtcgctc
     6061 ggcgagaagc cttccagaga gctacagagc ggggcgaagc ccggacaggt ctccaccttg
     6121 taacggaagt tcgtgttgcc ggccgacagg ccgatggctg ccggatcgac cgacaggaac
     6181 atgacgttgt tgttgaagac aacggtgtcg aactgcgccg cgctcgcggc gttcagcagc
     6241 cagcccgagt taaaagccac ggaacccggc ggagtgaaga ccagggtgat gaagatgtcc
     6301 tgggcgctgg cgttggcgtt gccgaagttg cgccgggcga tggcgccggg gctggtgttg
     6361 acgacgacgc gctcgtaggt tccgttctcg tcagcgtcga tgaagatgct gtaggccacg
     6421 tcgttcggcg ttgaccactc ttcgtagctg gcgacgccga agcgaatccg gttggtcgtc
     6481 gcgtcgtagg ccacgccgac gttgcggatg tcttgctcac ccgaaatgtc ggtgtccctc
     6541 gggctgacca cctgaagctc gaacgggctg accagcgaga cgacatccac ggggaatgaa
     6601 cccgtgcacg tcggcccggc ggcgcgtgtg ccggtgcaca cgtccgtccc gttcaaagcg
     6661 atggacgacg cgcccgccgg gattgagtcg gtggcggtca tggtcgaggt cggccgggcg
     6721 ggcgagtaga cggggagtcg tatcctcgta gcgcccgcga tcttgaagtt gatgtaggcc
     6781 ccttcttccg tcaggtagtg acgcgggaaa ttggcgaaca cgacgttgaa cgggctggct
     6841 cccgtctggg tcgccgggat gctcgggtcg cgcgtgtgat ccatctggtc ttcgaccgcg
     6901 tccatccgca ccgggaagac gaaggtttga ccggcctcca ccgtgatggt ggtcccctgc
     6961 gggagcgagt agctgacccc cggcgcatcg ctcagcgtgt cgatttcgag ggtgtaggtg
     7021 atctgggtgg tgcccttgtt gacgacgcga accggcttga ttctggtcgc accctggacc
     7081 acttcattgt cgaaactgac gctcactagg ccgtgatcga gggcgttgta ggcgacgacc
     7141 tgagtcctgg ccgcgctcgg cacgtcgagg cgtcccgcac cgatctggcc ggggccgcgc
     7201 cgggtgcccg cgccgccgac gtcggtgaag acgtcgcgga tggcgccgtt catcacctgc
     7261 gccttcaact cctccaccgt ccatgtcgga ttgagttcgc ggagcagagc catcgcgccc
     7321 gcgacgtgcg gggcggccat cgaggtgccg ctgatggtca gggtttggtt attcggttgg
     7381 aaaccggagg cgctggccac acctgtgcaa ccggccgcct gagcagtcaa gcaggtgacg
     7441 ccggtctgca ccgaggtgat gtcctgtccc ggcgcggaga tgtccggctt gagcgcgaag
     7501 atggccgacg tgccgccgcc gcgcgggccg cgggacgaga aggacgagat ggtgtccgac
     7561 cccggaatcc tgcgcaggct gacgttgacg gtgttgccgc cggtgatcga cgccttgatg
     7621 gcgttggcgt cggcctgttg aatgaggacc gtgggaatcg tgttggaact cgtgatggtc
     7681 gggtcgtccg agagattaga gacaccggcg acgttgttgg caatcatggt gccaatcgcg
     7741 cccgcatcct gtgcgttctt cgccttttgt tcaaaggcgc accggccgcg gtcaaccagc
     7801 acgatcttgc cgacgacggc ggcggcgttt gtcagcgggc tgcacccgtc cgtggtcgtc
     7861 ccggcggcca gccctaaacc ggggtcggcg ggttcggcaa cgtcgatgcc gaccaccacg
     7921 tcgcccgtca cgtcgaacaa ctgcggcccg aaaagcgcat tgcccactgt caaatcgcgg
     7981 acgatggcgg gcggggagtt aatgcgcgag cggttcacgc cgacgcccgg atcgagaaca
     8041 ctggcgacgc tgatggcccg cccggagacg ccgggggcgc cgctgatgaa gaaggtgtcg
     8101 ccggcgttac ccgccgaggc gaccacgatg acgccggcgc ggacggcgtt gtcgctcgcc
     8161 agcgccgagg ggttcgaggg cacgccgaag ttggagccga gcgacatgtt gatcacgtcg
     8221 aggcggtctt caaagtcgcc gtcgccgttc gggtcaatcg cccactcgat ggccgccacg
     8281 gtcaggttgg tgccgccgtt gcagccgaag acgcgaagcg cgtagaggct cgcgcggggc
     8341 gcgacgccgg ggccgatgcg gagcgagttg aaaggcgtgt tcgtcccgta ggggccggtg
     8401 aaggtggtgc cgtcggcgtt gacgccgaga ccggcggcgg tgccggcgac gtgcgagccg
     8461 tggccgttac agtccatcgg gtcggggtcg ggggcggcgg tggcggcgct gccgttataa
     8521 gcgtcacccg cgaagtcgaa gccgccggcg accttctgcg tcgggaagaa gccgtccacg
     8581 ttctgcgtgc ggttgttcgc cgtgtaatcg gcgagcagcc cacggccgcc gaagtttgaa
     8641 tgctggtagt caataccggt gtcgatgatg ccgaccttga ttccggcccc catcacaggc
     8701 cccccaggag cgccggcgga gttgttccac aattccgggg cgccgacgaa gggcacgctc
     8761 gtcgagttcg tcgggtattc gagcacgagc aggtgcacgg ctttgacgcc gggcagttgc
     8821 cgcagtgcgt cgagctggcc gggcggtacg gagacggcga tgccgttcag gacgcgctgc
     8881 acgcgataga tttccgtggc gtcgtatttg ccgcccaaca gggttgcggc gagcgtctcc
     8941 tgcgcacggg ttattttagt cagttgcgcg cgcgccgcgt tggtcgcctc ggtcatcgcc
     9001 gcgtcgcgcg gcagcggcga gctttccaac ttgtctgcgt agaccgtcgt cgccggggcg
     9061 tcggtcaact cgacgagcac ggcgacctgt ccgtcgctat tcgtcgccgc ggctatctcc
     9121 gcggaggaag cctcctggac ttgtgtcggt tcgtccacag tggcactacc ggaaacttgt
     9181 tcctggggcg tattgcccat tgactcaact gtctttgtct tttgcgcaaa ggcgtttgat
     9241 ccggtcgtga aagaagacca gagcaagctg ccagtgagtg ttagggcaaa gcacaggtaa
     9301 aggaaggtgc gagtgtttag gcggggtgct ttcatttctt ccctttcgtt ttgtggtcaa
     9361 gcttctgcgt ggcagtgctt gaggtgtgtt gcagactatc cttgccgggg ctggcgactg
     9421 cgttctaacg aactcggctg cctctgattc gagctacgca cggactgaga ggaaacgcag
     9481 gtcgttccgt agacatctcg accaacagcc gcgaggcgat ttgttattga cgacgcttca
     9541 ctagccctgc gtggagggtc aacgccctcc atcagacaat tctcgctcac gcgagagccg
     9601 cacgcagact cgtagataac cgtcggtttt agacgttgta tctaaatcaa tgaaacactt
     9661 agccgggggg gaactagcct tattatccac tgctcaaaac taatgcctcg gcatggcgtc
     9721 gtgtatatgg atgtttccga aggttgtctg gggagaccat gggaaatagc agcccacgaa
     9781 tgccaactca ttgactgatt aggcgaagac tctacaactg attcgttttc aagtcaacgc
     9841 gtatgcgggc agtgtcttca atccttttag ctgtacgcga ggtatcgaga gagtctcagc
     9901 ggcgagggcg agggagtacg tgcggcaaca caggtcgtgc tgccgcggtg agccgggggc
     9961 ggcggcgcca ctacgaccgg acggctaccg ggcagtgtca catgagagcg cgggatgcag
    10021 gagcggccga cgcgtcgccg accttagagc cgctgcccgc cctctatccg ggcgacgatc
    10081 cgctcgcaca gggcgtgggt ttgaagcgcg tctcgcaggg cggacggggc atcgccgcgc
    10141 ccgccagcca gttcgaggaa gtgttcgacc atctgcggga agccgcgccg gtggagcacc
    10201 gtgtcccagt tcctgaagcg gcggacgcgc tcttcccctc ccgtgtagtg cgtggtcacg
    10261 tccaaccccc gcacgtccca cttgttaccg gggctcatca cctctaagac ttcttcgtcg
    10321 gccccgctat cacggttcat gacgccgaag gcggcgaagc cgtcgccctc cagatgaagt
    10381 gtcaggtgtc gtagcagtcc ctcctgcgtg aagaaggtca cgcgctctgc cagagcttca
    10441 gccgggcaga ggaagcgcag tgtatcggcc acgtggatga aatcgtcgaa gacgaaacgg
    10501 cgggggtgtt ccggcaggta cgcgcggttc ttctgcatga ggacgagccg ccgtccgggc
    10561 ttagccttga ggccggcgta catcggggcg aagcggcggt tgaagccgac cgcgaggacg
    10621 cgacccgccc gctctgccgc ctcaaccacc gcgacggact cctcgtaagt gtaggagatc
    10681 ggcttgtcga ggtggacgtg gaggccgccc ctcagcagtt cactggccac ttcggcgtgt
    10741 gactcggtcg aggtgtggac gaaggccgcc tcgacgcccc ggcccggcaa ctcccgataa
    10801 tccgtagtga cctcggagac gcggtacgct ctcgatagac ggccaagtgt ctcccggtcg
    10861 cgtgtgcaga agatcaactc cacgtcgccg cgcgccgcga ggacgggcag gtaagccttc
    10921 cgcgcgatgt cacccagccc gaccacagcc acccgcatct gaatctccct tccccgtgac
    10981 gcgctccggc ccgcgatcac tgccgcactc ggaatttttt tgccgatgac gggcgcgctg
    11041 gggagcgccc cgaactcttc aggttacacc aagggttttc ccttatgcgt gctatcacac
    11101 atacaaaaag aaattttttg acattggatt caaaaaggca gaagcgcaaa gtaggaaact
    11161 tccgtccaca tttctcgagg agaagggttt ggattgaccg ttcctccgtt acttgaacaa
    11221 gcgtaccgct tcaagcggtt cgcgccaact tcagggaaag ttccaaccct tgaggtcgcg
    11281 gggtagatgt ccgctcccgg ccgcatggca accatattcc tattgcgggc ggccggcttc
    11341 ggcacaatca gggggataca taaatgaacg acgactacga cgagacctcg gggcctgagt
    11401 tgacggccga cgaggaggcg ctgttaaagg agatgccgac gctgaccgat gtcgggcagg
    11461 cgcggcgcaa cttcctggga cagacgattg ccggcggcct cggcgctttc gccctgatgc
    11521 tgttggagaa ggagcaggcg ttagccgcgc tcgccactgc gcccgacgcg gcgttcgccc
    11581 cggcggcgtc gctggagaat cccctcaggg tcgtcctgaa ggtcaacggc gcggcgaaga
    11641 cgctcgaagt tgactcgcgc atggtgctgc tcgacgcact gcgcgagcgg ctggagctga
    11701 ccggcacgaa gaagggctgc gaccaggggc agtgcggggc ctgcacagtg ctcgtcgatg
    11761 gtcggcgcgt gctgtcgtgc ctgacgctcg ccgccacggt cgaggggcgc gaggtcacga
    11821 ccgtcgaggg actggccagg ggcgatgagt tgcacccggt gcagacggcc ttcatcaagt
    11881 acgacggctt ccaatgcggc tactgcacgc cggggcaaat ctgctcggcc gtcgggctgc
    11941 tcgaagaggc gcgccgcggc gaggtgagcc acgtcaccga ggacgtgacc gtgacgacga
    12001 cccgtctcac ggacgacgag atacgcgagc ggatgagcgg caacatctgc cgctgcggcg
    12061 cttatccggg gatcgtcgcg gccatccggg aggcgcactc cgggagggag acggcgcgga
    12121 cgtggcgctt cgtcgaggag gaggagttgg cgcgggtcag aaaggagtgg gaagccgatg
    12181 agaccgttta agtacacgcg cgcgagcaac ccggcagcgg ccgcccgcgc ggtagcattg
    12241 aacccgcggg cgaagtttct ggcgggcggc accaacatcc tcgatctgat gaaggaggac
    12301 gtggagcgcc cgaacgagct tgtagacctc acgcgtctcg ggctgacgga gatcaaggct
    12361 gcccgcgacg gcggcatctc catcggcgcg ctggcgaaga acacggacac cgccaatcac
    12421 ccgctcatcc gtcagaacta cccgctgctc acgcaggcca tcgtcgcggg cgcctcgggg
    12481 caactccgca acatggccac gaacggcggg aacctgatgc agcgcacgcg ctgccagtat
    12541 ttctacgaca cggcgatgcc ctgcaacaag cgtgagccgg gcacgggctg cggcgcgctc
    12601 gaagggctca accggattca cgccatcctc ggctggagcg acaaatgcgt cgccacctat
    12661 ccgggcgaca tggcgaacgc gctctccgcg ctcgacgcgg tcgtgagggt gaggggcgcg
    12721 ggcgggcgcg agcgcacgat accaatcggc gagttccacc gcctgcccgg cgacacgccc
    12781 gagcgggaca acaacctcca gcacggcgaa ctcatcgtgt cgattgatct gcccaagtcc
    12841 aacttcgcga agaactccta ctatctgaaa gtccgcgacc gcgcaagcta cgccttcgcg
    12901 ctcgtcgccg tcgcggccgc gcttgaaatg aacggtggcc gaatcagcca ggcgcgcgtc
    12961 gtgctcgggg gcgtggccca caagccctgg cgaagcccgg aggcggaagc cgcgctctct
    13021 ggaagaccag cgtcggagga gagcttccgg caggccgccg aggcggcgct caaagacgcg
    13081 aagccgctcg cgcataacgc ttacaaagta gagctgggga agcgcgccat cgttcgctcc
    13141 ctgatgcgcg cgagcaggct ggcctgacag accaaggggt tgccggtctt taacctggag
    13201 gggacacgat gccaaggtac atcgggaaag agatgagccg cgtggacggc gtggcgaagg
    13261 tgacgggcag ggccaagtac gcggcggagt tccgcgcgcc gaacctcgcc tatggcttca
    13321 tcgtgctggg gagcgtcgcc aagggcacga tcaagtcgat agacacacgg gaggccgaaa
    13381 gcgcgacggg cgtcgtccgc gtcttcacgc acctgaacgc gccgaagctc gggccgaagg
    13441 cttccacgga gcaggcgccc ccgcgcgcca ctgaggagca ggacaagtcg ttccgggcgc
    13501 tccagtcgga cagaatcttc ttcaacgcgc agcccgtagc gctggtcgtc gccgagacct
    13561 acgagcaggc gcgctacgcg gcgcgactcg tcaaagtctc ctacaacgcg gagaagcacg
    13621 agactgacac cgaggccgtg cgcgggcgcg cgcgcgtccc atcacagggg ccgcccccga
    13681 agccgcgcgg caaccccgag gaggcgatga agcgagcgcc cgtgaaggtc gaggccgagt
    13741 atcgcatccc catcgagcac cataacccga tggagccgca cgctgccgtc gccgtctggc
    13801 agggcgacag gctcacggtc ttcgacaaga cgcagggggt ctacggcgtg cgtgagcacc
    13861 tggcgtcgag cttcggcgtc cccgagaaaa acatctcggt catctcgcca tacgtcggcg
    13921 gcgccttcgg atcgtccttg cggcccaact actacccggc gctcaccgcg atggccgcgc
    13981 gcgagatcaa gcgcccggtc aaggtcgtct acacgcgcac gcagatgttc accggccacg
    14041 gctatcgccc ctatacgatt cagaagatcg cgctcggggc cgaagcgtca gggaagctct
    14101 ccgcgatgat ccacgaggcg acacacaaca cctccgccct cgaagagttc tcggacgaca
    14161 ccacacgttt cacgcgacag gtctacgcgt gcccgaacct ctacgcgccg ctgaagatta
    14221 ccgacacgga cctccccacc ccgacatgga tgcgcgcgcc cggagcggtg agcggcatgt
    14281 tcgcgctcga atgcgcgatg gacgagctgg cctacgcgct caagatcgac ccgctggagt
    14341 tgcggctcat caactacgcc gaggtggacc cggagagcgg caagccgttt tcgagcaagg
    14401 cgctcaggga gtgctaccgg ctcggcgccg agaagttcgg atggaaggag cgcaagttcg
    14461 agccgcgctc gatgcgcgac gggcggctcc tcgtcggctg ggggatggcg accggagtct
    14521 ggggcgcgtt tcagatgccc gccacggccc gcatcacttt taaggcggac gggacggcgc
    14581 acgtcgccag cgcgacgagc gacatcggcc ccggaaccta cacggttgtc accatgattg
    14641 ccgccgagta cctggggctc aagcccgagc aggtgaagtt tgaactcggc gactcgaatc
    14701 ttcctcgcgc cccttcgcag ggcggctcct ggacgacggc gagcgtcggc tcggccgtcc
    14761 acggcgcggc cctcgccgtc ggcgcgcggc tgctggcgct ggccaacggg gaggcgaact
    14821 cgccgctcaa gggcgttgcg gtcgcggacg tggagatgct cgacggccgg ctgcgactca
    14881 aaagcgaccc gtcgcgtttc gtcagcgtct ccgaagtcat gaaacgcaac ggcctcgccg
    14941 agatcatcga gactttcgag gcgcgcccct ctaaagagcg cgagaagtac gcgacgctgg
    15001 cgcatggggc gcagtttgtc gaggttaaga tcgaccccga tctcgggaac gtccgcgtca
    15061 cgcgcgtcat cgaggtgacg gcctgcggca agatcatcaa ccccaaggcg tcgcacagcc
    15121 aggagatcgg cggcgtggtc tggggcatcg ggatggcgct gcaggaggcc accgaaatcg
    15181 accaccgtta cggacggatc atgaatccga acctccagca ctaccacgtc cccgtcaacg
    15241 ccgacatcca catgatcgaa acgatgttcg tcgaggagga cgacaagatt gtcaacccgc
    15301 tcggggtcaa gggcatgggc gagctcggga tggtcggcat ccctgccgcc attgccaacg
    15361 cggtctttca cgcgacaggc cgtcgcatca gggatctgcc gatcacgccg gacaagttgc
    15421 tgtagagggg gctgagctta tgaacgaagt gcgggcattg gtcgaagcgt tcg
//
LOCUS       DASVES010000142        34340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_220775, whole genome shotgun sequence.
ACCESSION   DASVES010000142 DASVES010000000
VERSION     DASVES010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 34340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34340
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220775"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VF240_05235"
     CDS             complement(<1..445)
                     /locus_tag="VF240_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174668.1"
                     /translation="MNALRDVSTFRPTDRFSDRVENYIKYRPGYPPGVLALMREEMAL
                     TPSSVVADIGSGTGLLARMFLQNGNAVYGVEPNREMREAGERLLEEFPSFKSVEGTAE
                     TTNLDGASVDFVTAGQAFHWFDVPRARREFQRILRPGGRVVLVWNN"
     gene            complement(452..1564)
                     /gene="mqnE"
                     /locus_tag="VF240_05240"
     CDS             complement(452..1564)
                     /gene="mqnE"
                     /locus_tag="VF240_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEX7174669.1"
                     /translation="MNFSHDTKLNELAAKVEAGERLTFADGVALYATDDLNALGRLAD
                     HVRRRLHGRTTYFNVNRHFNPTNVCYADCKFCGFFRTPRQPDAYTHNIDDSLRIAGEA
                     VAEGATELHIVGGLNTKLPFSYFTDLLSSLKRQYPRLHLKAWTMVELDHFAGFYKMSD
                     EDVIERLKMAGMDSCPGGGAEIFAEPTRSKICAHKTDGARWLELAGKVHAAGLKTNAT
                     MLYGHIESVEDRVDHLVRLREQQDASGGFQCFIPLAFYPPGTQLAHLPGPSGVDSLKT
                     MAVSRLMLDNFPHVKSYWVMLGKRLAQAALHYGANDLDGTITEGGELSESYSVESGGE
                     VKMSKQEIVRLIEDAGFEAVERDTLYNRVERETVVA"
     gene            complement(1608..2222)
                     /locus_tag="VF240_05245"
     CDS             complement(1608..2222)
                     /locus_tag="VF240_05245"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7174670.1"
                     /translation="MTATLIPTEQQADALVIHFSPPLRRLSDDEFFDFCMANKDLRIE
                     LTSAGDLIIMPPTGGKTGIRNFELIGYFWAWTNKDGTGRGFDSSTVFSLPNGAKRSPD
                     LSWVKKERWESLTEKEREKFSPICPDFVVELRSPSDSLRHLQQKMEEYIANGAQLGWL
                     IDPFERKVHVYRPQANVEVLDDPETVPGEPLLPGFTLDVRALFE"
     gene            complement(2264..3133)
                     /locus_tag="VF240_05250"
     CDS             complement(2264..3133)
                     /locus_tag="VF240_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEX7174671.1"
                     /translation="MWTAARQLSTTLEMIKIEHTLFALPFALLGAVLAAGGLPGAWQL
                     LWIALAMVGARSCAMAFNRIADRHIDAANPRTRTRALPAGQLSLPFAWAFTILSAALF
                     LFAAAMLNRLALALAPVALASVVLYSYTKRFTSLSHVVLGWCLAIAPTGAWVAVRGSL
                     DSAVPLLLSLVVLLWTAGFDVLYACQDYDFDRRTGLRSIPARVGVERALWASRLLHAA
                     AFAALVGLYFLTSLGWPALAGVLATGALLVYQHSLVRADDLSRLNAAFFTTNAFVSVI
                     LFATFGGAVLLRT"
     gene            complement(3226..3663)
                     /locus_tag="VF240_05255"
     CDS             complement(3226..3663)
                     /locus_tag="VF240_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174672.1"
                     /translation="MSQHEWRRGEYEISAERARLDLRFVHEYLSTRSYWARGRDTETV
                     RRSVEHSLPFGLYHGERQIGFARVVTDRATFAWLADVFVLDEFRGEGLGKWLVETILA
                     YPELQGLRRWTLGTRDAHELYRRFGFAETPAGFFMEKLDTSAK"
     gene            complement(3703..4350)
                     /locus_tag="VF240_05260"
     CDS             complement(3703..4350)
                     /locus_tag="VF240_05260"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEX7174673.1"
                     /translation="MELTVAITGASGTVYAHRTLLHLAASGVVERINLITSGTALVVA
                     RVEMGVSLREGDARSAGEWVGLPAESKLIRWHRLDNLAATPASGSHPQSGMIIVPCSM
                     GTLGAVASGAGTNLIHRAADVTLKEGRPLVVVPRETPYNTIHLENMLRLARAGARLLP
                     ASPGFYHRPQTIDELVDHLVFRILDQFGVPHPTGTRWKGDPTAAPREATVVVEED"
     gene            complement(4409..4894)
                     /locus_tag="VF240_05265"
     CDS             complement(4409..4894)
                     /locus_tag="VF240_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174674.1"
                     /translation="MELSGAAQTGGAKPERKAPSCSKSGPQKKKGLFGMNFAVPGEMK
                     MKKVRDVDYVLFTIHPKGNKEERLELWSGPHVGSGYPEQELRSSSEGIVERKWSCPEN
                     GGTDISGRTRDGKYWRTTTMFEGFARYEKVSEETSRKFDQIIDGMCCDAEFFRKLTGG
                     R"
     gene            5134..5889
                     /gene="larB"
                     /locus_tag="VF240_05270"
     CDS             5134..5889
                     /gene="larB"
                     /locus_tag="VF240_05270"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX7174675.1"
                     /translation="MDLQEIERLLRAFKQGELDEAEAARRLGNLHFEDIGHTRIDHDR
                     ATRQGFPEVVFGAGKTRRQIVEVVERLAARSPNVLITRTDADTYGEVRNVCNEAEWHE
                     SARMIRVWRDREARGAGLVVVVTAGTSDIPVAEEAALTAEALGSRVERVWDAGVAGIH
                     RIVSERERLRRARVVVVAAGMEGALPSVVGGLVAVPVVAVPTSVGYGASFGGVAALLG
                     MLNSCSSNVTVVNIDNGFGAGFVAGLISRNSDK"
     gene            5972..7465
                     /locus_tag="VF240_05275"
     CDS             5972..7465
                     /locus_tag="VF240_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174676.1"
                     /translation="MLIALAMGNAGCGARRTPDLERIFAGARERTGKRPVIVVPGILG
                     SQMVNRRTGEVVWPSILRTSGDGLSLPVSPELAANRDELVSSKILDTLKLGRLAPDVY
                     IYYELLKALERYGGYREGDWENPPADGDRDTFYVFAYDWRRDNVETAREFVRRVESLK
                     ERLGRPDLKFNVVAHSMGGLVARYAAMYGDSDLPAEGEIVRPDWAGASFVNKIFMFGT
                     PNEGSAEAFTTLLEGYSITEGLRRRVRLLNNLSREDAFTIPSIYQLLPHGEAARFLDA
                     ELRPFELDLYDPVVWRRYGWGALGDAAFRERVARAASRQGSNSNGSDGQTGPAGANTS
                     DDAAGAAGVAALARHEAYLAAALRRARRFHEALDALDTSAPPPVALFAFGGDCEETLS
                     APIVLRDEERARWLTLTEPRALRTPSGRKLKRREVTLAMYEPGDGRVTRRSLMGEGLG
                     SRRGSILFNTPLPVAYAVFACDLHSELHNNKTLQNNALTLLINELTH"
     gene            7503..9110
                     /locus_tag="VF240_05280"
     CDS             7503..9110
                     /locus_tag="VF240_05280"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX7174677.1"
                     /translation="MLTKRTARHALALLVLCLCPAPALARDNYPRAADLDALHYRIRL
                     EIKEGSDEIAADTEILFAAAADTPRVVALDFGRLTVDAVTEEGGPAEFTRAGEKLNVA
                     LKGTYRRGERFRIGVRYHGRPADGLLIKRNKFGDFAVFADNWPNRAHHWFPSIDHPYD
                     KATSEFFVTAPARFDVVSNGTLVETTSRQDGTRLTHWSERVPIPVYCMVIGVADFSIL
                     HRGEWERVPLSYYLFPKDRDKGVAAFGRAGEMIEFYTRLIGAFPYEKLALVQSSTRFG
                     GMENSSSIFFSEKGIDREGGVEGTVAHEIAHQWFGDSVTEADWNHLWLSEGFASYFGA
                     LFFEHFDGRDKFLSVMLAARERYFERSTPDFRPIHDPSITDLFKLLNGNNYQKGSWVL
                     HMLRRRVGDRAFFDGVRDYYRTYRDRNALTEDFQRIMEFHSGQRLEWFFRQWIYQSGH
                     PVYEASWRWDEPSKQLTLRVRQTQPGPLFRVPLDVEIQTGATAKLEVVEVDEREETFT
                     FKLDAKPTSVRVDPDEWVLKVLTLREQ"
     gene            complement(9395..11149)
                     /locus_tag="VF240_05285"
     CDS             complement(9395..11149)
                     /locus_tag="VF240_05285"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX7174678.1"
                     /translation="MRWSQFFAPTLRDDPADAEVVSHRLLLRAGYIRQLGAGIYSILP
                     LGWRVAQRAMRVIREEMDAIGGQEFFLPAMHPSALWEESGRWTAMGETMFRLKDRKGA
                     DLCLGMTHEEVFTSVARHEIRSYKQLPQVWYQIQTKFRDEERPKSGLMRLRQFIMKDA
                     YSFDVDQAGLDKSFNDQREAYKKIFDRCGIKYAIVEASSGSMGGSESHEFMARTNAGE
                     DFIATCEACGYAANLEKATSRLALVEDDAGPDAPEEFPTPGVRTIEDLTTFEGGATAD
                     RQIKSLVYVATDAATNEQYPALALLRGDHPLHETKLADALGAAAVRPAQPEEIRELLG
                     ASAGSLGGVGARARTQAAGRKFYIVADEALRGRRNMTTGANKDDHHLRGVNIERDIRP
                     DSWADLRAVAAGETCPRCERGTLEVFKAMEIGHIFKLGTKYSESMGARVLTQEGKEAP
                     IVMGSYGIGVERILSAAVEQSHDADGIVWPKTIAPFDVVVTVTNIKQAEILEAGERLY
                     AELRAAGLEVLLDDRDERAGVKFKDADLIGVPFRVTVGKKVGEGKVELFERASKQSSD
                     VSLDEVARKVQELALSGV"
     gene            complement(11317..12420)
                     /locus_tag="VF240_05290"
     CDS             complement(11317..12420)
                     /locus_tag="VF240_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174679.1"
                     /translation="MRFLSIFAPRLLAACALVAFTISAGAVEAPARWPSPQDKDKKEA
                     KAPPKAEQELADQIDKAPDAAAKFVVAEQFVKKFPKSQIRPKVVEHLAVQAFSPPDVP
                     QKIALAERLISLFPEAGESALVAPFLVDAYIAAQRFDDAFRAGGAWLGKSPNDVRVLT
                     VLSFHSVNLAQRGNSKFVAQGQPYGPKAIELLEAGTKPADMTAELFKEFKDSWLPQLY
                     QTQGLLSLVSGKTDEAFAKLQKAVGLNPSDPITHYLLGTARNEEYTKLATQIKTMSAA
                     AQKTEMTKVNALLDEIIDHYAHTVALAEGVKEHEQLRAQVLQDMTSYYKFRHNGSTDG
                     MQQLIDKYKKPAAAPATPAPAATPAATPAAPPR"
     gene            complement(12632..13768)
                     /locus_tag="VF240_05295"
     CDS             complement(12632..13768)
                     /locus_tag="VF240_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7174680.1"
                     /translation="MKRLKSTLPLAAFAFVCLLFPLDCLGQPQGRTQPDLPIDAAARK
                     QVIDGILKRLNEAYVFPEVAKKMETSVRERVAKGEYDSVASSQAFAEKLTADLQSVSK
                     DKHLRVRYSHDILPVRPERGEPTAEQREERRRELGWMNHGFDKVERLPGNIGYIEFRG
                     FFDEEMGAETVAAAMNFVGGTDSLIIDLRRNGGGNPSMVALISSYLFGPEPVHLNSLY
                     WREGDRTDEFWTRKEVAGKRYLGKDVYVLTSNRTFSGAEEFAYNLKNLKRATIVGETT
                     GGGAHPGGGFRASEHFMAFVPTGRAINPITKTNWEGTGVEPDVKVAADHALKTAHVAA
                     LRKGLNKVRDERLKRDMQDLIDNLQKELTDAQSKSAGDSQAKKN"
     gene            complement(13877..14539)
                     /locus_tag="VF240_05300"
     CDS             complement(13877..14539)
                     /locus_tag="VF240_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7174681.1"
                     /translation="MPATATREEIKDSILDATDRLLARYGYRKMTIEDIAREVGIGKG
                     SIYLHFESKEQVVLSHIDRIVERLKERLWAVARSEATAGVRLRLMLMARVLFRFDSVQ
                     HYTQSLDDLLAALRPNVLARRARYFDDEAQIFAQVLNDGRDSGEFIFDDALSAAYALL
                     HATNSLLPYSLSTSELGERDEVKEKTTQIADLILHGLLLRDPSRTVNRQSSIVNRKSK
                     EG"
     gene            14893..17436
                     /locus_tag="VF240_05305"
     CDS             14893..17436
                     /locus_tag="VF240_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174682.1"
                     /translation="MKNLLRACALSASLPLLLVSSSCDRKETARNQNDPAATPDRADA
                     TPTPPGEQTQATRTETPYRVKPVFLPELQRPPLTPNREALPRGVGGDLPTYDGEPFQL
                     NFGADQKIEPGRSGELVNQVLSALGWQRGAEEVRESRRAAAPAARGEAVEEAIKRAVA
                     EDERRLSGEMGRLSEATKKEIRERAERLRAEAARTQDVLVYDQRVDGVRVEHTGFVLA
                     FRPDRGLTSLSGRVFNRVDIANQRRLTEEDAARLAAEHVSKTTKLAPPPDAGAQQQPP
                     PRPELVLLPYADAMRYAWRVDVAAEEGVYRLWLDAESGEVLQLEPLFKPDSGNGLVFD
                     PDPNVGTRELHFEVNGPSASNYSLVNSRIDVGNSGADGQSSGDLTIADASTGGANFNV
                     PPINGTVVRRTSQTDYNSRFQEVNAFAWVAHTMKYVDDWGGTALPDMTVTVNHNNPCG
                     FGTNNACGGGGSITMGIGRATTSASTSANDFFNSAIDATVVTHELGHTLTPTQMTGGG
                     TLIGSADEGLADYWAATIHNNPTFGGFWGANRATHSQTGFPPRLAETLDVFPEHRNMG
                     GAGSNNQVHADGEIIRWALWSARTGMLSLSSLGSADLNAKLFKALNTHGVGLTNNLTD
                     RSVHDSYRDLLAKLAPQYTTGNANKLMAGFARAGIHLSERDAVIDISDDWLERGSATG
                     PTFTVWGGRDYQFNMGSNTATAATNFNTRYQIEVANDAAFTSNLISSGFLTNIAVGAD
                     GVPSALWALPAANWTTLKAADKIYYRVTTTDGAGANSRSSLQPGNNFLGNAVDPPYAN
                     INESGQCECECASAAVVPTAKSFLSWATLLPPLAALVWRRRSRRKGDAG"
     gene            17361..18323
                     /locus_tag="VF240_05310"
     CDS             17361..18323
                     /locus_tag="VF240_05310"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7174683.1"
                     /translation="MGDASAAAGRARLAPSLAPEGGRGVRPRLVEYLNALFGTTAFEW
                     LVPEPPVVYALAMLAVLCVIVRRAGRSGLTRYHVAGAALWAMLGGLVGARVFFLVQHL
                     GETLANPWQALDPAGGIASWGAYLGGLAGLALYCLVYKQDLPRHADLVASALGLGVAV
                     GRLSCFLNGDDFGTLSSAPWAVSFPHASYPFVAQARAALISPLADLSLPVHPVQLYLS
                     LNGLVLFLLASRFYKSFAPWPGATFCFYWLCYGTARFALEFFRGDQSAHLLFGALTVP
                     QVLCLLSLPAAAFGLRRSLRRADEPGSSVSVVAAPTKAAAQVST"
     gene            18470..19771
                     /gene="glyA"
                     /locus_tag="VF240_05315"
     CDS             18470..19771
                     /gene="glyA"
                     /locus_tag="VF240_05315"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7174684.1"
                     /translation="MNDLFNAPLSEVDPVISDAIDREVARQAEGLELIASENFVSEAV
                     LEAMGSVFTNKYAEGYPKKRYYGGCEWTDVVEQAAIDRAKQLFSAEHANVQPHSGAQA
                     NMAVLLAALQYGDQILGMNLSHGGHLTHGHPLNFSGISYKVADYGVSTETEQIDYDEL
                     QKIAEEHKPKLIICGASAYPRTIDFARIGEIARAIGARVMADIAHIAGPVVAGLHPSP
                     VPHADYVTTTTHKTLRGPRGGLILCREEHAKEVDRKVFPGVQGGPLVHIIAAKAVAFG
                     EALTEEFKDYQRRILANARALAAEMQEQGFRLVSGGTDNHLILVDVFMGGKGITGKVA
                     EKALEAAGITVNKNTIPFDTNTPFTASGVRIGTPALTTRGMGEDEMRQVARLIAAILH
                     EPESEEVRAQVRQSVHELSARFPLYPKRLKQRPETEAMGAD"
     gene            19911..20225
                     /locus_tag="VF240_05320"
     CDS             19911..20225
                     /locus_tag="VF240_05320"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX7174685.1"
                     /translation="MLKSVEEQIRKAMAEGEFDNLPGRGQPLSLEAYFSTPEHLRMAY
                     SVLKSGDFVPQEVQMFRDIDDLKTRLNSSTDAAERERLQKQIREKKLNLTVLLEGRRP
                     GR"
     gene            20320..22293
                     /locus_tag="VF240_05325"
     CDS             20320..22293
                     /locus_tag="VF240_05325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7174686.1"
                     /translation="MEEIFGPQGLIARAHPDYEYRPGQVQMADAVLRAFEERRHLVVE
                     AGTGVGKTLAYLVPAVAAALERRCRVVISTGTKNLQEQLMEKDIPFLQSVLPRKFTAA
                     YMKGRNNYSCLQRIKRAESTPILDGLDDLDHFDTVRRWARDSETGDRAELTELPENLP
                     FWRHIDARSETCLGQKCPDFDPCFITRMRQRASDADIIVVNHHLFFADLALRGGDYGQ
                     VIPDYGAVIFDEAHQIEDVAAEYFGAQVSNYQIEDLLRDLSNLPITDASTNRELTRTA
                     NRVARFSEGFWMGFTRDERGEEGRFPILPGTFARKGRSGEIEATPLGDSYLSLDGALG
                     RMEATLDALRDAPPEVENLLRRIREARFQLEFVVAGDDRRYVYWSERRGRGVFLRASP
                     IDVSTLLQEKLFERTETVVLTSATLASGGRFSFIRERLGLPEETDELIAQSGYDYDRQ
                     AVLYLPPRMPDPRSPQWAEAAADEVVKLLDLSEGHAFVLSTSLSGMQALYERVSPRVS
                     YPCFVQGSASKAGLLEKFRKTPNAVLFATSSFWQGVDVRGRQLSCVIVDKLPFAVPTD
                     PVVAARQRFIDEQGGSSFYEYSVPQACISLKQGLGRLIRGTTDRGLLAVLDPRLRTQG
                     YGRVFLASLPPCRITSDLEEAARVFETDAATVR"
     gene            22344..22565
                     /locus_tag="VF240_05330"
     CDS             22344..22565
                     /locus_tag="VF240_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HEX7174687.1"
                     /translation="MNRDEREDTTIYKVVVNHEEQYSIWPAERENALGWNDAGKSGTK
                     AECLAYIQEVWTDMRPLSLRKKMEETGKQ"
     gene            22659..23042
                     /gene="queD"
                     /locus_tag="VF240_05335"
     CDS             22659..23042
                     /gene="queD"
                     /locus_tag="VF240_05335"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX7174688.1"
                     /translation="MAAVFEVMIERNFSSAHQLRGYRGKCENLHGHNYRVEIYARGSE
                     LNNIGLLVDFVELKEAADEIVAYLDHKNINELPPFDEELNPSAENLARFILERVAARV
                     GDDRVQIHQVRCFETPTSVATYRVD"
     gene            complement(23150..24877)
                     /locus_tag="VF240_05340"
     CDS             complement(23150..24877)
                     /locus_tag="VF240_05340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7174689.1"
                     /translation="MLTLYDALLAQHPRQLTPVRCAEQTIAQLHRYFEDVVLENNLAA
                     LVVEGLPFAAERSLRDWERVREVGRAARHAFFFVSPDDALHSRPLKWDAIGRDPSLIK
                     LAGETSLEERFVVVADARFSALLATVRNRAGDDADAGGAEVIWTFEPDIVYSALEYLM
                     ARVTAECPAQAPLFSRAVRTSVPKATSLQLTVAVTTKLARLLQEQALREIAVNRIATA
                     IRSSLELSSVLQTTVDEVGRALGAQHSALCVEDEPGEPPLMHCYFRDGESDESEREGM
                     VSDLHAYQVRLGARMKAYVQDGCAGADADPTTRPVAAVPLIYRERAVGVLMVRSDDPS
                     RVWDENQILLMRTVADQVAVAVNHARLFEEMQQQALTDGLTGCPNRRSFEMQLKRDML
                     MATRTHHSLSLIMLDIDHFKRVNDTHGHAAGDVALRMIADGLRQELRGVDTAARYGGE
                     EFAVILPQAGLEGALKVAERLRARIEELDIPGVGGVTASFGVATFPAHAGARDLLVTT
                     ADRALYEAKHTGRNRVCIPPESELEADDSPAKLDDTDESEDAPRAEANAAATVDDGSI
                     LIALEDVSM"
     gene            complement(25089..25529)
                     /locus_tag="VF240_05345"
     CDS             complement(25089..25529)
                     /locus_tag="VF240_05345"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7174690.1"
                     /translation="MEQQLDETQLARLREIFGRIPYTRLLGMEFVGATRGAARIALEL
                     REELTRNAGLMHGGAVASLMDTASAFAVMSLLEPGQSTVTIDLTIHFLRPVLRGRIEA
                     RARVLRAGRRIVTLSIDTTDETGKLTATATTTYLIQTETPAQTP"
     gene            complement(25529..25939)
                     /locus_tag="VF240_05350"
     CDS             complement(25529..25939)
                     /locus_tag="VF240_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEX7174691.1"
                     /translation="MNNELQKRVPYEISSTKFSVSPQMVTTAFELPGYRVRQNLGVVR
                     GIIVRSRNLFVNIGAFFNTLAGGNINAWTRLCEETRAVAFEIMIQHATELGANAIIGA
                     RYDATEVIQGATEVLAYGTAVVVEPLEGTDLYNS"
     gene            complement(25975..26370)
                     /locus_tag="VF240_05355"
     CDS             complement(25975..26370)
                     /locus_tag="VF240_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX7174692.1"
                     /translation="MSPEKSENLDPDSPRYRERVYEIVRRIPAGRVMTYGQIADILGE
                     GYTARTVGFVMHAADEERDPWQRVINAQGACSTGRVILPPDRQQRMLEAEGIAFDARG
                     RCDLGRYRWTPEEFADGRTPDEQGSLFAD"
     gene            complement(26424..26786)
                     /locus_tag="VF240_05360"
     CDS             complement(26424..26786)
                     /locus_tag="VF240_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7174693.1"
                     /translation="MFDKVTLSEKFKLFDDRWSPKIVGELNDSYVKLVKIKGEFVWHQ
                     HEAEDEMFLVVKGRLTIRLRDRDIHLEEGEFVIVPRGVEHLPVADEEAHVLLLEPKTT
                     LNTGNVTSERTVAELQRV"
     gene            complement(26821..27498)
                     /locus_tag="VF240_05365"
     CDS             complement(26821..27498)
                     /locus_tag="VF240_05365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7174694.1"
                     /translation="MPLYDISVPIAPGVTPVYSGDPGIEIESWAALRRGDPANVSMLH
                     FGAHTGTHVDAPAHFIEGAPGITEVALDALVGRARVVELPADVRAIDAGHVAADVPEG
                     TPRVLFKTRNSAFWADARGRFREDFTYIAPDAARALARRGVRLVGIDYLSVEQFGSKD
                     FETHLTLLSKGVVILEGLDLSAVAAGAYELLCLPLKIAAGDGDGAPARALLRTLDDNG
                     NSPRPSA"
     gene            27693..28481
                     /locus_tag="VF240_05370"
     CDS             27693..28481
                     /locus_tag="VF240_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEX7174695.1"
                     /translation="MAASYYLNSAPLIWSFARGPRREQVELLTDAAPARCARMLARGA
                     AEAALVPVIECQSIEGAAVVPGVCVGAREEVQSVVLVTRGAGLEAVRSVALDTTSRTS
                     AALVRIIFREFLRREPEYVPAEPDVEAMLERSDAALVIGDPAMTFARAGLRVYDLARL
                     WREHTGLGFVFAVWMARDDGASRIRQVDFAAARDEGLARAEEIAAFYEGRLGRPRAEL
                     LKYLRENICYELNEEMRRGLELFYELAHKHKLIEGLKRLGFVDS"
     gene            28507..29487
                     /locus_tag="VF240_05375"
     CDS             28507..29487
                     /locus_tag="VF240_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174696.1"
                     /translation="MKYLSAVKVLMVAFLLFTFQTIKSQEIEYKGWDNGIGHSSWDIG
                     LGKEEKEKMLQLWDSIGEDLKTEQNELAGTYLKGGYSAGYFFRWSINKGYVLIPYFDQ
                     NLITDFSYGKVTFVDSSEVVFTPERDLKGGRSIGKMPRKWTAIGGYFVPVEMVKDFGD
                     YMAGFGVYNEFNGQCCAFAPNFLAQRIDRQEEKIDHPVSPKYARFIKPPIKADITFVG
                     IKKTVKDWGYDGELYSQWMERAVLIPVKISAGRAHGVKRNMLFRLIGEPDFYQYLQIM
                     RVHQRTASGYIVRDISFRGKETYTDSDTDQEKPLPPIKVGTKVTTSLVRN"
     gene            29536..30687
                     /gene="mqnC"
                     /locus_tag="VF240_05380"
     CDS             29536..30687
                     /gene="mqnC"
                     /locus_tag="VF240_05380"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEX7174697.1"
                     /translation="MKETNIQPILDKALSNIRLTAEDCTALLESHDIARIGVAADEVR
                     RRRHPDGVVTYIIDRNINYTNVCNVVCTFCAFYRRPGKPDTYVLEIDEVCRKIDETIA
                     LGGTGVLMQGGLHPDFRLEWYEDLLRTLHAKYPDFQLHCFSPPEIHNIHLISKLPYEE
                     IMGRLKAAGLYSLPGGGGEILDDEVRKRVSTKCTTDEWLDVMRAVHRVGLRSTATMMF
                     GIGDRTEHRVRHLQRVRDLQDETGGFTAFIPWTFQRENTALGRRIKDEPTGMDYLKML
                     SVSRLFLDNVEHIQSSWLTQGLRLGQVALRFGADDMGSIMIEENVVSAAGASNHADEQ
                     MLRYLIREAGFRPQQRDILYRHVNRNDMPNDSDAGGSAKLSELTVAFAD"
     gene            complement(30977..31387)
                     /locus_tag="VF240_05385"
     CDS             complement(30977..31387)
                     /locus_tag="VF240_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174698.1"
                     /translation="MRHKCVPLVLVLAMLTLAGCKKDAEIESVIAELHTFSEQLVSKV
                     QSAPNPSAGVDEAQKLMDSRKADLQAKMGSLKNVRGYQVSKETQQKMTDTLTKDAMNV
                     AGLQVKYVSVSMRDPAFKAKIEKLVKDYTEIFKM"
     gene            31752..32981
                     /locus_tag="VF240_05390"
     CDS             31752..32981
                     /locus_tag="VF240_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174699.1"
                     /translation="MRSIRFTSVLGAAVIMCAAVAASAQTGGEWPQWRGANRDGVSKE
                     TGLLKQWPADGPALAWKGVGAGMGYSSVVVSKGRLYTLGLRGDKEYVIAFDAATGKEV
                     WATAHGAAFRNDRGDGPRGTPTVDGERLYSLGGAGDLSAIDAKTGKIVWQMNVLQKFG
                     GSNITWGISESPLVIGDKVLVNPGGPGASVVALNKKDGTLIWKSQSDRAGYSSAIPVQ
                     VGGKTQVVFFTHTRALGLDAETGKLLWDYPKAANNVANSATPVVRGNRVWISSDYGTG
                     GGVVEIKADGTAAEVYFTKEMRNHHSTSILMGDHLYGFSGGILTAMKFDTGQVAWKDR
                     SVGKGSLVYADGMLYLFSENGVVGLAEATPTGYTEKGRFRIPQDNLPTWAHPVVAGGR
                     LYLRDQDTIYAYDVKAK"
     gene            complement(33289..34188)
                     /locus_tag="VF240_05395"
     CDS             complement(33289..34188)
                     /locus_tag="VF240_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174700.1"
                     /translation="MINLRRTALLICALALAVVAPAAQPRPQTAAGQNPARERVLLGL
                     KLRPEVAAWLEEVERKLGREVYAEFAQLGPEDGGEGFVGGENYVTERGAAVIRINFNF
                     QTAANRKRLEATLTHEILHLRQRARGFPAFMFDEAGSSPRVPLGELNEMELTGALMEA
                     VEHHAILPDLHRLGLDTVADMIGGLAWTRGKGVSPDHPLHAIYYLRAMLEYNDPARLR
                     ELEQIYRRNRWARSLQRGARIAQIIRSASLSTPAEMTTVYLRCLTELFGGLYRFTARP
                     APSQFPRDRIFPTIPVAVTFTRR"
BASE COUNT         6060 a        11123 c        11163 g         5994 t
ORIGIN      
        1 ggttgttcca gacgaggacg acacggccgc cgggccggag gatgcgttgg aactcgcgcc
       61 gcgcgcgtgg cacgtcgaac cagtggaacg cctgacccgc cgtgacgaaa tcgacgctcg
      121 cgccgtcgag gttcgtcgtc tcggccgtgc cttcgacact cttgaagctc ggaaattctt
      181 ccagcaggcg ctcgcccgcc tcgcgcatct cacggttcgg ctcgacgccg tagaccgcgt
      241 tcccgttctg caaaaacata cgcgcgagca gccccgtgcc ggagccgatg tcggcgacga
      301 ccgacgacgg cgtgagcgcc atctcttcgc gcatgagcgc gagcacaccg ggcgggtagc
      361 cggggcggta cttgatgtaa ttctcgaccc ggtcggaaaa cctgtcggtg ggccggaaag
      421 tggaaacgtc tcgcaatgcg ttcatcaaac tttaagcaac taccgtctcg cgctcgacgc
      481 ggttgtagag cgtgtcgcgc tccacggcct cgaagccggc gtcctcgata agccggacga
      541 tttcctgctt cgacatcttg acctcgccgc cggactcgac cgagtagctt tcggaaagct
      601 cgccgccctc ggtgatcgtg ccgtcgagat cgttggcccc gtagtggagc gcggcctgcg
      661 cgagccgctt gcccagcatc acccagtagg acttgacgtg cgggaagttg tcgagcatga
      721 gacgcgagac ggccatcgtc ttcaacgaat cgacgcccga ggggccgggg aggtgcgcga
      781 gctgcgtgcc cggtgggtaa aacgcgagcg ggatgaagca ctggaagccg cctgaagcgt
      841 cctgctgctc gcgcaggcgg acgaggtgat cgacgcggtc ttcgacggat tcgatgtggc
      901 cgtagagcat cgtcgcgttg gtcttgaggc cggcggcatg caccttgccg gcaagctcca
      961 gccagcgcgc gccgtcggtc ttgtgggcgc agattttcga gcgtgtcggc tcggcgaaaa
     1021 tctccgcgcc gccgccgggg caggagtcca tcccggccat cttcaaacgc tcgatcacgt
     1081 cttcatcaga catcttgtag aagcccgcga agtggtcaag ctcgaccatc gtccacgcct
     1141 tgaggtggag acgcgggtat tgccgcttga gcgaggacag aaggtctgtg aagtagctaa
     1201 acggcaactt ggtgttgagg ccgccgacga tgtgcagctc ggtcgcgccc tcggcgacgg
     1261 cctcgccggc gatgcgtaga gagtcgtcga tgttatgcgt gtaggcgtcg ggctgccggg
     1321 gcgtgcggaa gaagccgcag aacttgcagt cggcgtaaca gacgttcgtc gggttgaagt
     1381 ggcggttgac gttgaagtag gtcgtccgcc cgtgcagccg gcgccggacg tggtcggcca
     1441 aacggccgag cgcgttcaaa tcgtccgtcg cgtagagcgc cacgccgtcc gcgaaagtga
     1501 gccgttcgcc ggcctctact ttggcggcga gttcgttgag tttcgtatcg tgcgaaaagt
     1561 tcatcgggtt aagttcaaag tttcaggttc aaaggtcgaa gttcatttca ctcgaagagg
     1621 gcgcgcacgt cgagcgtgaa gccggggagc agtggctcgc cggggacggt ctcaggatcg
     1681 tcgagcactt cgacgttcgc ttgcgggcgg tagacgtgaa ccttccgctc aaacgggtcg
     1741 atgagccaac cgagctgcgc gccgttggcg atgtactcct ccatcttctg ttgcagatga
     1801 cggagcgaat cgctgggcga gcgtaactct acgacgaaat cggggcagat cggagaaaac
     1861 ttctcccgct ccttttccgt cagagattcc catctttctt ttttgaccca cgagagatcg
     1921 ggcgacctct tagcgccatt cggcaacgaa aaaacggttg aagagtcgaa gccccgtccc
     1981 gtcccatctt tattggtcca agcccaaaag tagccgataa gctcaaagtt tctaatgcct
     2041 gtctttcctc cagtgggcgg catgataatg aggtctcctg cgcttgtgag ttcgatgcgg
     2101 aggtctttat tggccatgca gaagtcgaag aactcgtcgt cgctcaacct tcgcaggggc
     2161 gggctgaagt ggatgacaag cgcgtcggcc tgctgctccg tcggtataag tgttgcggtc
     2221 ataactgcac gttctttctt cgagagaaac acgtctctcc gcttcaagtc ctcaagagca
     2281 ccgccccgcc gaacgtcgcg aacaggatga cgctgacgaa ggcgttggtc gtaaagaacg
     2341 cggcgttgag ccgtgaaaga tcgtcggcgc gcacgagcga gtgctggtag acgaggagcg
     2401 cgcccgtggc gagcaccccg gcgagcgcgg gccagccgag cgaagttaga aagtataacc
     2461 caacgagggc ggcgaacgcc gccgcgtgga gcaggcgcga agcccacagg gcgcgctcga
     2521 cgccgacgcg cgccgggatc gagcgcagcc ccgtgcggcg gtcgaagtcg tagtcctgac
     2581 aggcgtagag cacgtcgaag ccggccgtcc acagcagcac gacgagcgag agcaggagcg
     2641 gcacggccga gtcgagcgag ccgcgcacgg cgacccacgc ccccgtcggg gcgatggcca
     2701 gacaccagcc gaggacgacg tgcgagagcg acgtgaagcg cttcgtgtag gagtagagca
     2761 cgacgctggc gagcgcgacg ggggcgagcg cgagcgccag ccgattgagc atcgcggcgg
     2821 cgaagagaaa gagtgcggct gaaagaatcg tgaacgccca ggcgaaggga agcgagagct
     2881 ggccggcggg cagggcgcgc gtgcgtgtgc gtgggttggc ggcgtcgatg tgccggtcgg
     2941 cgatgcggtt gaaggccatc gcacaggagc gcgcgccgac catcgccagc gcgatccaca
     3001 acagttgcca cgctccgggg agcccgcccg ccgccagcac cgccccgagc agcgcgaagg
     3061 gcagcgcgaa gagcgtgtgc tcgatcttaa tcatttcgag cgtcgtgctt aactgtcggg
     3121 cggccgtcca catcgtttgc aaatcgcctc tgaatcaact ctccggttcg cactccgcca
     3181 ggacgcgcgc gcgacacttg ccgcgccgcg tgtggcattg agccgtcatt tcgcggaagt
     3241 gtcgagcttt tccatgaaga agccggcggg cgtctccgcg aagccgaacc gccggtagag
     3301 ttcatgtgca tcccgcgtgc cgagagtcca gcggcgcagg ccctgcaatt cggggtacgc
     3361 cagaatcgtc tcgacgagcc acttgcccaa gccttcgccg cgaaactcgt cgaggacgaa
     3421 cacgtcggcc agccacgcga aggtggcgcg gtcggtgacg acgcgcgcga aaccgatctg
     3481 ccgctccccg tggtagaggc cgaacgggag cgagtgctcg accgagcgcc gcacggtctc
     3541 cgtgtcgcgc ccgcgcgccc agtagctccg cgtgctcagg tattcgtgga cgaagcgcag
     3601 gtcgaggcgc gcgcgttcgg cgctgatctc atactcgcct cgccgccatt cgtgttgaga
     3661 catttccgaa gacaccatcg acagaactcg ccccacgcgt cttcaatcct cttcgacgac
     3721 gaccgtcgcc tcgcgcggcg cggccgtggg gtcgcccttc cagcgcgtcc ccgtcgggtg
     3781 cgggacgccg aactgatcga ggatgcggaa gacgaggtga tcgacgagtt cgtcaatcgt
     3841 ctgcggccgg tggtaaaagc cggggctggc cggaagcagg cgcgcgccgg cgcgtgcgag
     3901 ccggagcatg ttctccagat ggatcgtgtt gtaaggcgtc tcgcgcggga cgacgacgag
     3961 cgggcggcct tccttgagcg tcacgtcggc cgcgcggtgg atgaggttcg tgcccgcgcc
     4021 cgaggcgacg gcgccgagcg tccccatcga gcaggggacg atgatcatgc ccgactgcgg
     4081 gtgcgaccca gaggcgggcg tcgcggccag gttgtcgagc cggtgccagc ggatcagctt
     4141 cgactccgcc ggcaggccca cccactcgcc cgccgagcgc gcgtcgccct cgcgcaagga
     4201 gacacccatc tcgacgcgcg ccaccaccag cgccgtcccc gacgtgatga ggttgatacg
     4261 ctcgaccacg ccgctcgccg ccagatgcaa aagcgtgcgg tgtgcgtaga cagtgcccga
     4321 ggccccggtg atggctacgg ttaattccat aaggagctgt cagcgttcag cgttcagcgt
     4381 tcagcttcgg gccgcttcca cggccggact aacgcccgcc cgtgagcttt ctgaaaaact
     4441 ccgcgtcgca acacatgccg tcgatgatct ggtcgaactt ccgcgacgtt tcttcagaaa
     4501 ctttctcgta cctcgcaaac ccctcgaaca tcgtcgtcgt gcgccagtat tttccgtccc
     4561 gcgtcctccc gctgatgtcg gttccgccat tctcgggaca actccacttg cgctccacga
     4621 tgccttccga cgaactccgc aactcttgtt ccgggtagcc gctgccgaca tgcggcccgc
     4681 tccacagctc aaggcgctct tccttgttcc ccttcgggtg gatggtgaag agtacgtaat
     4741 caacatcgcg caccttcttc attttcatct cgccgggcac ggcgaaattc atcccgaaca
     4801 gacccttctt cttttgcggg ccggactttg agcaggaagg cgctttgcgc tcgggcttcg
     4861 caccgccggt ctgtgcggcc ccgctcagtt ccacggcgaa acagttcaca cagcagcaga
     4921 gtaggacgct cagaaggagg caaaactgct gacctgaaat gagcgagcgt tgccgcggga
     4981 gggtcataac acgtcctgtc agaaacaagt agctcgacga catgatacgg gccgccggcc
     5041 gccgagggca agaacaccaa atttaatgaa cgcgcccgca gatgataaga tgattcccca
     5101 atcctttgtg gagacgaacg ctaccgagac gcgatggact tgcaggaaat agagcggctg
     5161 ctccgagcct tcaaacaggg cgagttggac gaggccgagg cggcgcgacg tctcggcaac
     5221 ctgcactttg aagacatcgg ccacacacgc atcgaccacg accgcgccac gcggcagggc
     5281 ttccccgagg tcgtcttcgg agcggggaag acgcggcggc agatcgtcga ggtggtcgag
     5341 cgcctcgcgg cgcgctcgcc gaacgtgctt atcacgcgca cggacgccga cacctacggc
     5401 gaagtccgca acgtctgcaa cgaggccgag tggcacgagt cggcgcggat gattcgcgtc
     5461 tggcgcgacc gcgaggcgcg cggggcgggg ctggtggtgg tcgtcacggc cgggacgagc
     5521 gacatccccg tggccgagga ggcggcgttg acggccgagg ctttgggcag ccgcgtcgag
     5581 cgcgtgtggg acgcgggcgt ggcgggcatc caccgcatcg tgtcggagcg cgagcggctg
     5641 cggcgggcgc gagtggtcgt cgtcgcggcg gggatggagg gcgcgctgcc ctcggtcgtc
     5701 ggcggcctcg tcgccgtccc cgtggtggcc gtgccgacga gtgtcggcta cggcgcttcc
     5761 tttggcggcg tggccgcgct gctcgggatg ctcaactcct gctcctccaa cgtcaccgtc
     5821 gtcaacatcg acaacggctt cggcgccggc ttcgtcgccg gactcattag tcggaacagt
     5881 gataagtgat gagtgataag tgatgagaaa gagaatgcag acaagggcca cgcgccctct
     5941 tcttatcact catcacttat cactcatcac tttgctcatc gcgctcgcga tgggcaacgc
     6001 cgggtgcggc gcgcggcgca cgcccgattt ggagcgcatc ttcgcggggg cgcgcgagcg
     6061 aacgggcaag cgccccgtga tcgtcgtccc cggcatcctc ggctcgcaga tggtcaaccg
     6121 gcgcaccggc gaggtggtgt ggccctcgat cctccgcacc tccggcgacg gcctgagcct
     6181 gcccgtctcg cctgagctcg ccgcgaaccg cgacgaactc gtctcctcga aaattctgga
     6241 cacgctaaaa ctcggccgtc tcgccccaga cgtttacatc tactacgagc tgctcaaggc
     6301 cctggagcga tacggcggct accgcgaggg cgattgggag aacccgcccg ccgacggcga
     6361 ccgcgacacc ttctacgtct tcgcctacga ctggcggcgc gacaacgtcg agacggcgcg
     6421 cgaattcgtc cggcgcgtcg agtcgctgaa ggagaggctg ggccggcccg acctgaagtt
     6481 caacgtcgtg gcccactcga tgggcggact cgtggcgcgc tacgcggcca tgtacggcga
     6541 ctcggacttg ccggccgagg gcgagatcgt ccgccccgac tgggccggcg cgtccttcgt
     6601 caacaagatt ttcatgttcg gcacgcccaa cgaaggctca gccgaagcct tcacgacgct
     6661 gctcgaaggc tactccatca ccgagggcct gcgccgccgc gtgcgcctcc tgaacaatct
     6721 ttcgcgcgaa gacgccttca ccatcccgtc catctaccag ttgctcccgc acggcgaggc
     6781 ggcgcgcttc ctcgacgcgg aactccggcc cttcgagcta gacctctacg accccgtggt
     6841 ctggcgtcgc tacggctggg gcgctctggg cgacgcggct tttcgcgagc gtgtcgcgcg
     6901 cgccgcgtcg cggcagggca gtaactccaa cggctcggat ggccagacgg ggccggcagg
     6961 cgcaaacact tcagacgacg cggcgggcgc ggcaggcgtc gcggcgctcg cgcgccacga
     7021 ggcgtattta gccgccgccc tgcgccgcgc gcgccgcttc cacgaagcgc tcgacgcgct
     7081 cgacacgagc gcgcccccgc ccgtcgcgct cttcgccttc ggcggcgact gtgaggagac
     7141 gctctcggcc cccatcgtgc tgcgcgacga ggagcgcgcg cgctggctga cgctgacgga
     7201 gccgcgcgcg ctccgcacgc cttcgggccg caagctcaag cgccgggagg tcaccctcgc
     7261 gatgtatgag ccgggcgacg gccgcgtcac gcgccgctcg ctcatgggcg agggcctcgg
     7321 ctcgcgccgc ggcagcatcc tctttaacac gcccctgccc gtggcttacg ccgtcttcgc
     7381 ctgcgacctc cacagcgagc tgcacaacaa caagaccctg caaaacaacg cgctcaccct
     7441 gctcatcaac gagttgacac attgacgggg ccgcgcggcc ccaccggaga ggcgaataga
     7501 aaatgttgac gaaacgcacc gcccgccacg ccctcgccct gctggtgctc tgcctgtgtc
     7561 cggcgcccgc gctcgcgcgc gacaactacc cgcgcgcggc cgacctcgac gccctccact
     7621 accgcatccg tctcgaaatc aaagagggga gcgacgaaat cgcggccgac accgaaatcc
     7681 tcttcgcggc cgccgccgac acgccgcgcg tcgtcgcgct cgacttcggc cgcctcaccg
     7741 tggacgccgt cacggaagaa ggcggccccg ccgagttcac gcgcgccggt gaaaagctca
     7801 acgtcgcgct caaaggaacc tatcgccgcg gcgaacgctt ccgcatcggc gtcaggtatc
     7861 acgggcgacc cgccgacggt ctcctcatca agcgcaacaa gttcggcgac ttcgcggtct
     7921 tcgccgacaa ctggcccaac cgcgcgcacc actggttccc ctcaatcgac cacccttacg
     7981 acaaggcgac ctccgagttc ttcgtcacag cgcccgcgcg cttcgatgtc gtctccaacg
     8041 gcacgctcgt cgagaccacc agccgtcagg acgggacgcg cctcacgcac tggagcgagc
     8101 gcgtccccat ccccgtctac tgcatggtca tcggcgtcgc cgacttctcc atcctccatc
     8161 ggggcgagtg ggaacgtgtg ccgctctcct actatctctt cccgaaagat cgtgataagg
     8221 gcgtcgccgc cttcgggcgc gccggcgaga tgatcgagtt ctacacgcgg ctcatcggag
     8281 cgttccccta cgagaagctc gcgctcgtcc agtcttcgac gcgcttcggc ggcatggaaa
     8341 actcgtcgag cattttcttt tcggagaaag gcatcgaccg cgagggcggc gtcgagggca
     8401 cggtggcgca cgagatcgcg catcagtggt tcggcgactc cgtgaccgag gccgactgga
     8461 accacctctg gctgagcgag ggcttcgcca gctacttcgg cgcgctcttc ttcgagcact
     8521 tcgacggccg cgacaagttc ctctccgtca tgctcgccgc gcgcgaaaga tacttcgagc
     8581 gcagcacccc cgacttccgc cccatccacg acccctcgat caccgacctc ttcaagctgc
     8641 tcaacgggaa caactaccag aaaggctcat gggtgctgca catgctccgc cgacgcgtcg
     8701 gcgaccgagc cttcttcgac ggcgtgcgcg actactaccg cacctatcgc gaccgcaacg
     8761 ccctcaccga agacttccag cgcatcatgg aattccactc cggccagcgg ctcgaatggt
     8821 tcttccgcca gtggatttac cagagcggcc atcccgtcta cgaagcctct tggcgctggg
     8881 acgagccgtc gaagcaactc accctgcgcg tccgccagac tcagcccggc ccactcttcc
     8941 gcgtcccgct cgacgtggag attcagaccg gcgcaaccgc caaactcgaa gtggtggagg
     9001 tggacgaaag ggaagagact ttcaccttca agctcgacgc caagccgaca agcgtgcgcg
     9061 ttgacccgga cgagtgggtc ttgaaggttc tgacgttaag agagcagtga gagccggcat
     9121 caacaagcga cacaagccga gttgcgaagc gcgcgaggag agcaagggcg atagcgagcg
     9181 ccggaggcgc ggaagaatgt agcccgtggc gtaagccacg ggaaccggca atggaaatga
     9241 gataagcccc ggaggggcga aagaatccta accggcgtga gacgcaaggt agattttctt
     9301 tcgcccctcc ggggcttcga ttttttatga gccgtgtacc cacggctcgc gccgtgggct
     9361 acattctttc gcccctgccg gggctgttca tcgattacac ccctgacagc gccagctcct
     9421 gcaccttccg cgcgacctcg tccagactca cgtcgctcga ctgcttcgag gcgcgctcga
     9481 acagctcgac cttgccctcg ccgaccttct tgccgacggt gacgcggaag ggaacgccga
     9541 tcaggtcggc gtccttgaac ttgacgccgg cgcgctcgtc gcggtcgtcg aggaggactt
     9601 cgaggccggc ggcgcgcagc tcggcgtaga gtcgttcgcc ggcttccagt atctcagcct
     9661 gcttgatgtt cgtgacggtg acgaccacgt cgaagggcgc gattgtcttg ggccagacga
     9721 tgccgtccgc atcgtggctc tgctcgacgg ccgccgacag gatgcgctcg acgccgatgc
     9781 cgtagctgcc catcactatc ggcgcttcct tcccttcctg cgtgagcacc cgcgcgccca
     9841 tcgactcgga gtacttcgtc ccgagcttga agatgtggcc gatctccatc gccttgaaga
     9901 cttcgagcgt gccccgttcg caacgcgggc acgtctcgcc ggccgcgacg gcgcgcaggt
     9961 cggcccaact gtctggacgg atgtcgcgtt caatattcac accacgcaga tgatggtcgt
    10021 ccttgttcgc gccggtcgtc atgttgcgtc ggccgcgcaa cgcctcgtcg gcgacgatgt
    10081 aaaactttct gccggccgcc tgcgtcctcg cgcgcgcgcc gacgccgccg aggctcccgg
    10141 cgctcgcgcc caaaagttcg cgtatctctt caggttgcgc gggcctgacg gccgcggcgc
    10201 cgagcgcgtc ggcgagcttc gtctcgtgca gcgggtgatc gccgcgcagg agcgcgagcg
    10261 ccgggtactg ttcgttcgtc gccgcgtcgg tcgccacgta gacgagcgac ttgatctgcc
    10321 ggtcggccgt ggcgccgccc tcgaacgtcg tcaggtcttc gatggtgcgg acgcccggcg
    10381 tgggaaactc ttcgggcgcg tcggggccgg cgtcgtcctc aacgagcgcg aggcgggaag
    10441 tggccttctc aagattggcc gcgtagccgc acgcttcaca ggtggcgatg aagtcttcgc
    10501 cggcattcgt gcgggccatg aactcgtggg actcggagcc gcccatcgag ccggaagagg
    10561 cttcgacgat tgcgtacttg atgccgcagc ggtcgaagat tttcttgtac gcctcgcgct
    10621 ggtcgttgaa agacttgtcg agtccggcct gatcaacgtc gaaggagtag gcgtccttca
    10681 taatgaactg ccgcaggcgc atgaggcccg acttggggcg ctcctcgtcg cggaacttgg
    10741 tctgaatctg ataccagacc tgcggcagtt gcttgtagga gcgtatctcg tggcgcgcga
    10801 cggaggtgaa gacttcttcg tgcgtcatgc cgaggcagag gtctgcgccc ttgcggtctt
    10861 tgagccggaa catcgtctcg cccatcgccg tccagcgccc cgactcctcc cacagagcag
    10921 acgggtgcat ggccgggagg aagaactcct gtccgccgat ggcgtccatc tcctcgcgga
    10981 tgactcgcat cgcccgctgc gcgacgcgcc agcctaaggg gaggatggag tagatgccgg
    11041 cgccgagctg gcggatgtag ccggcccgca gcagcaggcg gtgcgagacg acttcagcgt
    11101 cagccgggtc gtcgcggagc gtgggggcga aaaactgtga ccagcgcata gtgttttaaa
    11161 ttcaactttc cttcgggcgt ctgtctgtcc gagataactg aaattaccca tccgcggcgg
    11221 agaacgcaaa gacaaaacgc ggaggacgca aagggtgaag ccgtttccct tgcgtcctcc
    11281 gcgtttgctt cgcacccttc gtgcggaaat ttttgtctac cgcggcgggg ccgcgggcgt
    11341 cgccgcgggc gtggccgcgg gggccggcgt ggccggcgcg gcggccggct tcttgtactt
    11401 gtcgatgagc tgctgcatgc cgtcggtcga gccgttgtgg cggaacttgt agtaggaggt
    11461 catgtcctgg agcacctgcg cgcggagctg ctcatgctcc ttgacgcctt cggcgagggc
    11521 tacggtgtgc gcatagtggt cgatgatctc gtcgaggagt gcgttgacct tggtcatctc
    11581 agtcttctgg gccgccgccg acatcgtttt gatctgcgtg gccaacttcg tgtactcctc
    11641 gttgcgcgcg gtgcccaaca ggtaatgggt gatggggtcg gacgggttga gtccgaccgc
    11701 cttctgaagc ttggcgaagg cttcgtcggt cttgccggag acgagcgaca aaaggccctg
    11761 cgtctggtag agctgcggga gccagctatc cttgaactcc ttgaaaagct cggcggtcat
    11821 gtcggccggc ttcgtcccgg cctccagaag ctcgatggcc ttcgggccgt agggttgtcc
    11881 ctgggcgacg aacttcgagt tgccgcgctg ggcgaggttg acggagtgga aggagagcac
    11941 cgtgagcacg cgcacgtcat tgggactctt gccgagccac gcgccgccgg cgcggaaggc
    12001 gtcgtcgaaa cgctgcgcgg cgatataggc gtcaacgagg aaaggcgcga cgagggcgct
    12061 ctcgcccgcc tcggggaaga gcgagatcag acgctcggcg agcgcaattt tctgcgggac
    12121 gtccggcggc gagaacgcct gcacggcgag gtgctcgacg accttcgggc gaatctggct
    12181 cttgggaaac ttcttgacga actgctcggc gaccacaaac ttggcggcgg cgtcgggcgc
    12241 cttgtcgatc tggtcggcga gttcttgctc agccttcggg ggcgccttcg cctccttctt
    12301 gtccttgtcc tgcggcgacg gccaccgggc tggagcctcg accgcgccgg cggaaatcgt
    12361 gaacgcgaca agggcgcacg ccgcgagcag acgcggcgcg aagatggaca ggaatctcat
    12421 cggtgacctc actcagaaag aatggttttg atggggaaag tttgccgcgc cttcggcggc
    12481 gcgacggtcg cattgtgctg gatgcgcgcg gcagttgcaa gctttagaga aggcgcggcc
    12541 cgttcggccg gcgcggcaaa aaaaccgagg gcgcgcacct tgaaaagtgc gcgccctcgg
    12601 ctgctcgcgg gtcgtgtcgc gggcgggcta actagttctt cttagcctgc gagtcgccgg
    12661 ccgattttga ctgcgcgtcc gtcagctcct tctgcaagtt gtcgatgagg tcctgcatgt
    12721 cgcgcttcag gcgctcgtca cggactttgt tgagaccctt acgcagggcc gcgacgtgcg
    12781 cggtcttgag cgcgtggtcg gccgccactt taacgtccgg ctcgacgccc gtgccctccc
    12841 agttggtctt ggtgatgggg ttgatggcgc ggcccgtggg gacgaaggcc atgaagtgct
    12901 cgctcgcgcg gaagccgccg ccggggtgcg cgccgccgcc cgtggtctcg ccgacgatgg
    12961 tcgcgcgctt caggttcttg aggttgtagg cgaactcctc ggccccggag aaagtgcggt
    13021 tgctcgtcag gacatacacg tctttgccga gataacgctt gccggcgact tccttgcgcg
    13081 tccagaactc gtcggtgcgg tcgccctcgc gccagtagag gctgttgagg tgaaccggct
    13141 ccgggccgaa gaggtaggag ctgatgaggg cgaccatcga ggggttgccg ccgccgttgc
    13201 ggcgcaggtc gatgatgagg gagtcggtgc cgcccacgaa gttcatcgcc gcggccacgg
    13261 tctcggcgcc catctcttcg tcgaagaagc cgcggaactc gatgtaaccg atgttgccgg
    13321 gcaggcgctc gaccttgtcg aagccgtgat tcatccagcc gagttcgcgc cggcgctcct
    13381 cgcgctgctc ggccgtcggc tcgccgcgct cggggcggac gggcagaatg tcgtggctgt
    13441 agcgcacgcg caggtgcttg tccttactca cggactggag gtcggccgtg agcttttcgg
    13501 cgaacgcctg cgagctggct acggagtcgt attcgccctt cgcgacgcgc tcgcgcacgg
    13561 atgtctccat cttcttagcg acctcgggga agacgtaggc ttcgttgaga cgcttgagga
    13621 tgccgtcgat gacctgtttg cgagcggcgg cgtcgatggg caggtcaggc tgcgtgcgcc
    13681 cctgcggctg gccgaggcag tcgagtggga agaggaggca cacgaaggcg aatgccgcga
    13741 gcgggagtgt ggacttcagt cgtttcatgt ttcgagtctc cttgatgttt taaaagccgt
    13801 gaatcgtcaa tcgtcaatcg tcaatcgcgt tttagtgtcg ctcgacttga gtagtgaatc
    13861 gggagcaaat gaaacgtcaa ccctctttcg atttgcgatt gacgattgac gattgacgat
    13921 tgacggttct tgatgggtcg cgcagcagga gtccgtgcag gatcaggtcg gcgatctgcg
    13981 tggtcttctc cttcacttcg tcgcgctcgc cgagttcgga cgtgctgagg ctgtagggga
    14041 gtagggaatt agtggcgtgc agcagcgcgt aggccgcgga gagcgcgtcg tcgaagatga
    14101 actcgccgga gtcgcgcccg tcgttgagaa cctgtgcgaa aatctgcgcc tcgtcgtcga
    14161 agtagcgcgc gcggcgcgcg agcacgttgg gccggagcgc ggccagcagg tcgtcgaggc
    14221 tctgggtgta gtgctgcacg ctgtcgaagc ggaagaggac gcgcgccatg agcatcaggc
    14281 gcagccgcac gccggcggtc gcctccgagc gggccaccgc ccacagacgc tccttcagac
    14341 gctcgacgat cctgtcgatg tgcgagagga cgacctgctc cttgctctcg aagtggagat
    14401 agatgctgcc cttgccgatg ccgacctcgc gggcgatgtc ctcgatggtc atcttccggt
    14461 agccgtagcg cgccagcagg cggtcggtgg cgtccagaat cgagtctttg atttcttcac
    14521 gtgtggcggt cgccggcatc agtttgactc ctgaccaaaa tcgctttcca gtcattatta
    14581 cgccgggacg gtgtcagagt tccaaaagtt ttcgcgtcgg gcggggcgct gtggcctttg
    14641 aggacgactt acgttccgcg cccgacgcgc ccacgcgcgg cggagaaaaa ccttgacaac
    14701 ccctgcgctc aggcataaat tcgcgccgca cttcaagggc aacgacttcg aggcacgccc
    14761 gccctcgccc cgcttccccg cgcgcgcgtc cttcgcgtct actggacttc aatcatcctt
    14821 gccgtagctt cggccggaca attcttcacc ctgcacacgc accggcggcc cgtttggagg
    14881 tgacccatga agatgaaaaa tttactgcgg gcctgcgccc tgtcagcgtc gcttccgctg
    14941 ctcctcgtct cgtcctcttg cgacaggaag gagacggcac gcaaccagaa cgacccggcc
    15001 gcaactcccg accgcgccga cgcgacgccg acgccgcccg gcgagcagac gcaggcgacg
    15061 cggacggaga cgccgtaccg ggtcaagccc gtcttcctgc cagagctaca acgcccgccc
    15121 ctcaccccta atcgcgaagc gctgccgcgg ggcgtgggcg gcgacctgcc gacctacgac
    15181 ggcgagccgt tccagctcaa cttcggcgcc gaccagaaga tcgagccggg acgctctggc
    15241 gagttagtca atcaggtgct cagtgcgctc ggctggcagc gcggcgccga ggaggtcagg
    15301 gagtcgcgcc gcgccgccgc gccggccgcg cggggtgagg ccgttgaaga ggccatcaag
    15361 cgcgctgtgg ccgaagacga acgacgcctg tcgggcgaga tgggtcgtct gagcgaggcg
    15421 acgaagaagg agattcggga gcgggccgag cgcctgcgcg ccgaggccgc gcgcacgcag
    15481 gacgtgttgg tctacgacca gcgcgtcgat ggcgtgcgcg tcgagcacac gggcttcgtc
    15541 ctcgccttcc gacccgaccg cgggctgacc tcgctcagcg gccgcgtctt caaccgtgtg
    15601 gacatcgcca accagcggcg gctgaccgag gaggacgccg cgcgcctcgc cgccgaacac
    15661 gtgagcaaga cgacgaagct cgccccgccc ccggacgcgg gcgctcagca acagccgccc
    15721 ccccggcccg agcttgtgct tctgccctac gccgacgcga tgcgctacgc gtggcgcgtg
    15781 gacgtggcgg ccgaggaagg cgtctatcgc ctctggctcg acgccgagag cggcgaggtg
    15841 ctgcagctcg aaccgctttt caagcctgac tccggcaacg gcctcgtctt cgaccccgac
    15901 ccgaacgtcg gcacgcgcga gctgcacttc gaggtcaacg gcccttcggc cagcaactac
    15961 agcctcgtca actcgcgcat cgacgtgggc aactccggcg ccgacggcca gtcgagcggc
    16021 gacctaacca tcgccgacgc cagcacgggc ggcgccaact tcaacgtccc cccgatcaac
    16081 gggacggtcg tgcggcgaac ttctcagacc gactacaaca gtcgctttca ggaggtcaac
    16141 gccttcgcgt gggtcgcgca cacgatgaag tacgtggatg actggggcgg cacggcgctg
    16201 cccgatatga cggtcacggt caaccacaac aacccctgcg gcttcggcac caacaacgcg
    16261 tgcggcggcg gcggcagcat cacgatgggc atcggcaggg cgaccacctc ggccagtacc
    16321 tccgccaacg acttcttcaa ctcggccatc gacgcgacgg tcgtcaccca cgaactcggc
    16381 cacacgctga cgccgacgca gatgaccggc ggcggcacgc tcatcggctc ggccgacgag
    16441 gggctggccg actactgggc cgcaaccatc cacaacaacc cgaccttcgg cggcttctgg
    16501 ggcgcgaacc gggccacgca ctcgcagacc ggcttccccc cgcgcctggc cgagacgctc
    16561 gacgtgttcc ccgagcaccg caacatgggc ggcgcgggca gcaacaacca ggttcacgcc
    16621 gacggcgaga tcatcaggtg ggcgctgtgg agcgcgcgca cggggatgct gagcctcagc
    16681 tcgcttggct ccgccgatct caacgccaag ctcttcaagg cgctcaacac gcacggcgtc
    16741 gggctgacca acaacctcac cgaccgcagc gtccacgact cctaccgcga cctcctcgcc
    16801 aagctcgcgc cgcagtacac caccggcaac gccaacaagc tgatggccgg cttcgcccgc
    16861 gccggcatcc acttgagcga gcgcgacgcc gtcatcgaca tcagcgacga ctggctggag
    16921 cgcggcagcg ccacggggcc gaccttcacc gtctggggcg ggcgcgacta tcaattcaac
    16981 atgggcagca acaccgccac ggccgccacc aatttcaaca cccgctacca gatcgaagtc
    17041 gccaacgacg cggccttcac ctccaacctc atttcgagcg gcttcctgac gaacatcgcc
    17101 gtcggcgcgg atggcgtccc ctcggctttg tgggcgctgc ccgccgccaa ctggacgacg
    17161 ctcaaggccg ccgacaagat ttattaccgc gtgacgacca ccgacggcgc gggcgctaac
    17221 tcccgctcct cgctccagcc cggcaataac ttcctcggca acgccgtcga tccgccctac
    17281 gccaacatca acgagagcgg acagtgtgag tgtgagtgcg cgtcggccgc ggtcgtcccc
    17341 acggcgaaga gtttcctgtc gtgggcgacg cttctgccgc cgctggccgc gctcgtctgg
    17401 cgccgtcgct cgcgccggaa gggggacgcg gggtgaggcc gcgcctcgtc gaatatctca
    17461 acgcgctctt cggcacgacc gcctttgaat ggctcgtgcc ggagccgccc gtagtctacg
    17521 cgctggcgat gctcgcggtg ctgtgtgtga tcgtgcggcg cgcggggcga tccggtctca
    17581 cgcgctacca cgtggcgggc gcggcgctgt gggcgatgct gggcggactc gtcggcgcgc
    17641 gcgtcttctt cctcgtccag catctcggcg agacgctggc gaacccttgg caggcgctcg
    17701 accccgcggg cggcatcgcc tcgtggggcg cttatctcgg cggccttgcg ggtctcgcac
    17761 tctactgcct cgtctacaaa caggatttgc cgcgccacgc cgacctcgtc gcctccgcgc
    17821 tcggactcgg cgtggcggtg gggcgcttga gttgcttcct caacggcgac gacttcggga
    17881 cgctttcgtc cgcgccgtgg gccgtcagct tcccgcacgc gagctacccc ttcgtggcgc
    17941 aggcgcgcgc cgccctcatc tcgccgctcg ccgacctctc gctccccgtc caccccgtcc
    18001 aactctatct gtcgctcaac ggcctcgtcc tcttcctgct cgccagccgt ttttacaaaa
    18061 gcttcgcccc gtggcccggc gcgaccttct gcttctactg gctctgctac ggcacggcgc
    18121 gcttcgccct cgaattcttc cgcggcgacc agtcggccca tctcctcttc ggcgcgctca
    18181 ccgtgccgca ggtgttgtgc ctcctgtcgc tgccggcggc ggccttcggc cttcgtcgga
    18241 gtctgcgccg cgccgacgag ccgggcagca gcgtctccgt tgtggccgcg ccgaccaaag
    18301 cggcggctca ggtctcgacc tgacacgccc acgctcgcgg tactgtgttg tgcccgcggc
    18361 gcgctccctc ccaagcgccg gcgcgatgcg ttgcacgctt ccggggcgtg aggtaacatc
    18421 caagccaaat caaagttcat gttaagttgt cgccgccggg aggtttgaga tgaacgacct
    18481 ttttaacgcg cccctgtcgg aagttgaccc cgtcatcagc gacgcgattg accgcgaggt
    18541 ggctcgccag gccgaggggc tggaactcat cgcctcagag aacttcgtca gcgaagccgt
    18601 gctcgaagcg atggggtcgg tcttcacgaa caagtacgcg gaaggctatc cgaagaaacg
    18661 ttattacggc ggctgcgagt ggacggacgt ggtcgagcag gcggcgattg accgggccaa
    18721 acaactcttc tcggccgagc acgccaacgt ccagccgcac tcgggcgcgc aggccaacat
    18781 ggccgtcctc ctcgccgcgc tccagtacgg cgaccagata ctcggcatga acctctcgca
    18841 cggcgggcac ctcacgcacg gccaccccct aaacttctcc ggcatcagct acaaggtggc
    18901 cgactacggc gtctccacag agaccgagca gatcgactac gacgagctac aaaaaatcgc
    18961 cgaagagcac aagccgaaac tgatcatctg cggcgcttca gcctacccgc gcaccatcga
    19021 cttcgcacgc atcggcgaga tcgcgcgcgc catcggcgct cgcgtcatgg ccgacatcgc
    19081 acacatcgcc ggccccgtcg tcgccggcct ccacccgtcg cccgtcccac acgccgacta
    19141 cgtcaccacg acgacgcaca agacgctgcg cggcccgcgc ggcggcctca tcctctgccg
    19201 cgaggagcac gcgaaggaag tcgataggaa agtcttcccc ggagtgcagg gcggcccgct
    19261 cgtccacatc atcgcggcga aggccgtggc tttcggcgag gctctgaccg aggagttcaa
    19321 ggattatcag cggcgcattc tcgccaacgc gcgcgctctc gccgccgaga tgcaggagca
    19381 gggatttcgc ctcgtctcgg gcgggacgga caatcacctc atcctcgtgg acgtctttat
    19441 gggcgggaag ggaatcacgg gcaaggtcgc cgagaaggcg ctcgaagccg caggcatcac
    19501 cgtcaacaag aacaccatcc ccttcgacac caacacgccc ttcaccgcct ccggcgtccg
    19561 catcggcacg cccgccctca ccacgcgcgg catgggcgag gacgagatgc gacaggtcgc
    19621 ccggctcatc gccgccatcc tccacgagcc ggagtccgag gaggtgcgcg cgcaagtccg
    19681 gcagtccgtc catgagcttt cggcgcgctt cccgctctac ccgaagcgtc tgaagcagag
    19741 gccggagacg gaggcgatgg gcgcggacta agtaaggaaa aagtaaaaag gcaaaaggca
    19801 aaagtgagga agcatcgcct cttcactttt gccttttgcc tttttacttt ttccttccat
    19861 cggtggtatg ccgtacaata cgcgcatcac gcgcgtggag gtgcggggcc gtgctcaagt
    19921 ccgtcgagga gcagattcgg aaggcgatgg ccgagggcga gttcgacaac ctgcccggca
    19981 ggggccagcc cctcagtctt gaggcttact tcagcacgcc cgagcacctc cgcatggcct
    20041 actcggtttt gaagagcggc gacttcgtcc cccaagaggt gcagatgttc cgagacatcg
    20101 acgacttgaa gacgcgtctc aactcctcga ccgacgcggc cgagcgtgaa cgactccaga
    20161 agcagatcag ggagaaaaaa ctgaatctca ccgtcctgct ggagggacgc cggcccggcc
    20221 gttgaaatca cgcggcccga acgacgacgc cgacgcccgg cccgcacccc tccccgttgc
    20281 gggccgatca gggaaacagc cctcactact gaataacaca tggaagaaat cttcggccca
    20341 cagggactga tcgcgcgcgc gcaccccgac tacgagtacc ggcccggtca ggtgcagatg
    20401 gccgacgccg tgctgcgcgc cttcgaggag cggcgtcacc tcgtcgtcga ggcgggcacg
    20461 ggcgtgggca agactctggc ctacctcgtc ccggccgtcg cggcggcgct ggagcgtcgc
    20521 tgccgcgtcg tcatctccac ggggacgaaa aacttgcagg agcagttgat ggagaaggac
    20581 attccttttc tccagagcgt gctcccgcgc aagttcacgg ccgcttacat gaagggccgc
    20641 aacaactact cctgcctcca gcgcatcaag cgcgccgagt ccacgcccat cctcgacggc
    20701 ttagacgacc tcgaccactt cgacaccgtg cgccgctggg cgcgcgactc cgaaacgggc
    20761 gaccgcgccg agctgaccga gctgcccgag aacctcccct tctggcgtca catcgacgcg
    20821 cgctccgaga cctgcctcgg acagaagtgc cccgacttcg acccctgctt catcacacgc
    20881 atgcgccagc gcgcctccga cgccgacatc atcgtcgtca accaccacct cttcttcgcc
    20941 gacctcgcgc ttcgcggcgg cgactacgga caggtcatcc ccgactacgg cgccgtcatc
    21001 ttcgacgagg cgcaccagat cgaagacgtg gccgccgagt acttcggcgc gcaggtctcc
    21061 aactaccaga tcgaagacct cctgcgcgat ttgagtaacc tgccgattac ggacgcgagc
    21121 acgaaccgcg agctgacgcg cacggccaac cgcgtcgcgc gcttcagtga gggcttttgg
    21181 atgggcttca cgcgcgacga gcgcggcgag gagggacgct tccccatcct gccgggcacg
    21241 ttcgcgcgca agggccgctc cggcgagatc gaggccaccc cgctcggcga ttcgtacctc
    21301 agtctcgacg gcgcactggg ccgcatggag gccacactcg acgcgctgcg cgacgcgccg
    21361 cccgaagtcg aaaacctcct gcgtcgtatc cgcgaggcgc gctttcagtt ggagttcgtc
    21421 gtcgcgggcg acgaccgccg ctacgtctac tggagcgagc gccgcggccg cggcgtcttc
    21481 ctgcgcgcct cgcccatcga cgtttccacg ctccttcagg aaaaactctt cgagcgcacc
    21541 gagaccgtcg tgctcacctc ggcgacgctc gccagcggcg gccgcttcag cttcatacgc
    21601 gaacgcctcg gccttcccga agagaccgac gagctgatcg cgcagtcggg ctacgactac
    21661 gaccggcagg ccgtgctcta cctgccgccg cggatgcccg acccgcgctc gccccagtgg
    21721 gccgaggcgg cggccgacga agtcgtcaag ctcctcgacc tttcagaggg ccacgccttc
    21781 gtgctctcga cttcgctctc cggcatgcag gcgctctacg agcgcgtctc gccgcgcgtc
    21841 agctacccgt gcttcgtgca ggggagcgcg tcgaaggcgg ggctgctgga gaagtttcgg
    21901 aagacgccga acgccgtgct cttcgccacg tcgagcttct ggcagggcgt ggatgtgcgc
    21961 ggccgacaac tgtcgtgcgt catcgttgac aagctgccct tcgccgtccc gaccgacccg
    22021 gtggtggccg cgcgccaacg cttcatcgac gagcagggcg ggtcgagttt ctacgagtac
    22081 agcgtcccgc aggcgtgcat ctcgctcaag cagggactcg gccgactgat tcgcgggacg
    22141 accgaccgcg gcctgctcgc cgtcctcgac ccgcgcctgc gcacgcaagg gtacggtcgc
    22201 gtcttcctcg cgagcctccc gccgtgccgc atcacctccg atctcgaaga ggccgcgcgg
    22261 gtgtttgaaa cggacgcggc aacagttaga taatgacggg gccgttctat aaacccgaat
    22321 aacctgtccc accggaggtg cgaatgaatc gagacgagcg cgaagacacg acgatctaca
    22381 aggtggtcgt caaccacgag gagcagtact cgatctggcc agccgagcgc gagaacgcgc
    22441 tcggctggaa cgacgccggc aagtcgggca ccaaggcgga atgtctcgcc tacattcagg
    22501 aggtgtggac ggacatgcgc ccgctcagcc tgcggaagaa gatggaagag acggggaagc
    22561 agtgaggtgc agccgccttt agcattcgag cgcgcgcggc gtgagcacac gtatctcgtg
    22621 ccgcgcgtgg cccgaagatc gaggggaagc aaaacgagat ggccgcagtc tttgaagtga
    22681 tgatcgagcg caacttttcg agcgcgcacc agttgcgcgg ctaccgtggg aagtgcgaga
    22741 acctgcacgg ccacaactac cgcgtggaga tttacgcgcg cggcagcgag ctgaacaaca
    22801 tcgggctgct ggtggatttc gtcgagttga aggaggcggc cgacgagatc gtcgcctacc
    22861 tcgaccacaa gaacatcaac gagctgccgc ccttcgacga agagctgaac ccctcggccg
    22921 aaaacctcgc ccgcttcatc ctcgaacgcg tcgccgcgcg cgtcggcgac gaccgcgtgc
    22981 aaatccatca ggtgcgctgc ttcgagacgc ccacgagcgt cgccacctat cgggtcgatt
    23041 gagacgcggc cccggccact cacctaaacg ttaagaggcg ggcacgtgcc cgcctcttaa
    23101 cgtttctaaa atggttcgct caccgctctc ggcgcggccc aaaactaaat cacatcgaca
    23161 cgtcttcgag cgcgatgagg attgacccgt cgtcaaccgt cgccgccgcg ttcgcctcgg
    23221 cccgcggcgc gtcctccgac tcgtccgtat catcgagctt cgccggcgag tcgtcggctt
    23281 ccagttcgga ttccggcggt atgcagacgc ggttgcggcc cgtgtgcttg gcttcgtaga
    23341 gcgcgcggtc ggccgtcgtc acgagcaggt cgcgcgcgcc ggcatgggcc gggaaggtgg
    23401 cgacgccgaa agaggccgtc acgccgccga cgccggggat gtccagttct tcgatgcgcg
    23461 cgcgcagtcg ctcggcgacc ttcagcgcgc cttcgaggcc cgcctgcggc agaatcacgg
    23521 cgaactcctc gccgccgtag cgcgccgccg tgtccacgcc gcgcagctcc tgtctcaaac
    23581 cgtcggcgat catcctgagc gccacgtcgc cggcggcgtg gccgtgcgtg tcgttgacgc
    23641 gcttgaagtg gtcgatgtcg agcatgatga gagagagcga gtggtgcgtg cgcgtggcca
    23701 tgagcatgtc tcgcttgagc tgcatctcga aggagcggcg gttggggcac cccgtcagtc
    23761 cgtcggtcag cgcttgctgc tgcatctcct cgaagagacg cgcgtggttg acggccacgg
    23821 ccacctgatc ggccaccgtc cgcatcagca gtatctgatt ctcatcccac acgcgcgaag
    23881 ggtcgtccga gcgcaccatc agcacgccca cggcgcgctc gcggtagatg agcggcacgg
    23941 cggcgacggg gcgcgtcgtc gggtcggcgt cggcgccggc gcatccgtcc tgcacgtaag
    24001 ccttcatgcg cgcgcccagt cgaacctgat aggcgtggag gtcgctgacc atcccttcgc
    24061 gctccgactc gtcgctctca ccgtcgcgga agtagcagtg catgagcggc ggctcgcccg
    24121 gctcgtcctc gacgcacagg gcgctgtgct gcgcgccgag cgcgcggccg acctcgtcaa
    24181 ctgtggtctg aagcacgctc gacaattcca aagagctgcg aatggccgtg gcgatgcggt
    24241 tgaccgcgat ctcgcgcagg gcctgctcct gtagcagacg cgcgagcttg gtcgtaacgg
    24301 cgacggtcag ttgcagcgag gtcgccttcg gcacgctcgt ccgcaccgcc cgcgagaaga
    24361 gcggcgcctg cgcggggcac tcggccgtca cgcgcgccat gaggtattcg agcgccgagt
    24421 agacgatgtc cggctcgaac gtccagatga cctcggcgcc gccggcgtcc gcgtcgtcgc
    24481 ccgcgcggtt gcgcacggtc gcgagcaggg cggagaagcg cgcgtcggcg acaactacga
    24541 accgctcttc gagtgaagtc tctccggcca gtttgatgag gctggggtcg cgcccgatgg
    24601 cgtcccactt cagtgggcgg ctgtgcagcg cgtcgtcggg cgagacgaag aagaaggcgt
    24661 ggcgggccgc gcggccgacc tcgcggacgc gctcccaatc gcgcaacgaa cgctcggcgg
    24721 cgaacggcag cccctcgacg acgagcgccg cgagattgtt ctccagcacc acgtcctcga
    24781 aataacgatg taactgcgcg atggtctgct cggcgcaacg cacgggcgtg agctggcgcg
    24841 gatgttgggc gagcagtgcg tcgtagaggg tgagcatggg ccgaagcgcg gaaagtgaag
    24901 gactgaaggc gcgaggttca aaggaagccc ggcggcttgg gaggccgtcc gcaagtcgga
    24961 cggcaggcaa acacattttt acatatgcca acaccgcttg tctataagat aaatgagtgt
    25021 gaaaaatgac ggggaaaagc caaatttctg acgcatgcag ttgatttggg acgcaaagaa
    25081 gggtggtgtt agggagtttg ggccggagtc tcggtctgga taaggtaagt ggttgtggcc
    25141 gttgcggtaa gcttgccggt ctcgtccgtc gtgtcgattg agagggtgac gatgcggcgg
    25201 ccggcgcgca ggacgcgtgc gcgggcctcg attcggccgc gcaggacggg gcggaggaag
    25261 tggatcgtga ggtcgatggt gacggtgctc tggccgggtt cgagcagcga catgacggcg
    25321 aaggccgagg ccgtgtccat gagcgaggcg acggccccgc cgtgcatcag gcccgcgttg
    25381 cgtgtcaact cctcgcgcag ttcgagcgcg atgcgggccg cgccgcgcgt tgctcccacg
    25441 aactccatcc cgagcaggcg cgtgtagggg atgcggccga agatttcgcg caggcgcgcg
    25501 agctgcgtct catcgagttg ctgctccatc aggagttgta gaggtcggtc ccttccaacg
    25561 gctcgacgac gacggctgtg ccgtaggcga gaacttcggt cgcgccctgt atgacctcgg
    25621 tggcgtcgta gcgtgcgccg atgatggcgt tggcgccgag ttcggtcgcg tgttgaatca
    25681 tgatctcgaa ggcaacggcg cgcgtctcct cgcacagacg cgtccaggcg ttgatgttgc
    25741 cgccggcgag cgtgttgaag aaggcgccga tgttgacgaa gaggttgcgc gagcggacga
    25801 tgatgccgcg cacgacgccg aggttctgcc ggacgcggta gcccggaagc tcgaaggcgg
    25861 tcgtgaccat ctgcggcgag acggagaact tcgtcgagct gatctcgtag gggacgcgtt
    25921 tttgcagttc gttgttcatc actaaagaaa gctccttttg tgagctgcgg ccgttcagtc
    25981 ggcgaagagg ctgccctgtt cgtcaggtgt gcgtccgtcg gcgaactctt cgggcgtcca
    26041 gcggtagcgc ccgaggtcgc agcggccccg cgcgtcaaag gcgatgccct cggcttcgag
    26101 catgcgctgc tgtctgtcgg gcggcaggat gacgcggccc gtcgagcacg cgccctgcgc
    26161 gttgatgacg cgctgccagg ggtcgcgctc ctcgtcggcc gcgtgcatga cgaagccgac
    26221 ggtgcgggcc gtgtacccct cgccgaggat gtcggcgatc tgcccgtagg tcatcacgcg
    26281 ccccgcgggg atgcggcgca cgatctcgta gacgcgctcg cggtagcgcg gcgaatcggg
    26341 gtctaagttc tcactctttt caggggacat cgtgggtctc actttcggcc gtatcggttg
    26401 tccgcgccgg actccttaca gactcaaacg cgctgaagct cggcgacggt gcgctcgctc
    26461 gtgacgttgc cggtgttgag cgtcgtcttc ggctccagca ggaggacgtg tgcctcctcg
    26521 tcggccacgg gcaggtgctc gacgccgcgg gggacgatga cgaactcgcc ctcttcaagg
    26581 tgaatgtcac ggtctcgcag gcgaatcgtc aggcggcctt tgacgacgag gaacatctcg
    26641 tcctccgcct cgtgttggtg ccacacgaac tcgcccttga tctttacgag cttgacgtag
    26701 gagtcgttca actcgccgac gatcttcgga ctccagcggt cgtcgaagag tttgaacttc
    26761 tcggagagcg tgaccttatc gaacaaagtt tgtctcctta gattctcacg gcccgaagtc
    26821 tcacgccgag ggccgaggcg agttgccgtt gtcgtccagt gtccgcagaa gggcacgagc
    26881 gggcgcgccg tcgccgtcgc cggccgctat cttcagcggc aggcagagca gttcgtaagc
    26941 gccagcggcg acggcgctga ggtcgagacc ttcgaggatc acgacgccct tcgagagaag
    27001 cgtcaggtgc gtctcgaaat ctttcgaccc gaactgctcg acggacaggt agtcgatgcc
    27061 gacgagccgg acgccgcgcc gggcgagggc gcgcgcggcg tcgggcgcga tgtaggtgaa
    27121 gtcctcgcgg aaacggccgc gcgcgtctgc ccagaaggcc gagttccgcg tcttgaagag
    27181 gacgcggggc gtgccctcgg gcacgtctgc tgcgacgtgg ccggcgtcga tggcgcgcac
    27241 gtccgcaggc aactccacga cgcgcgcgcg tccgacgagc gcgtcgagcg cgacctcagt
    27301 gatgccgggg gcgccttcga tgaagtgtgc gggggcgtcc acgtgcgtcc ccgtgtgcgc
    27361 gccgaagtgg agcatcgaga cgttggccgg atcgccccgg cgcagcgccg cccacgactc
    27421 gatttcgatg ccggggtcgc ccgagtagac cggcgtcacg cccggcgcga tggggacgct
    27481 gatgtcgtac aggggcatag gcagtgacgg ataacgaatg actggtgacg agttcagttc
    27541 gggattttaa cttgtcaccg gtcactcgtc cacctgtctt gcggcgcgcg gcgggcgtgg
    27601 gttagcatcg gggccggcta cagcaagcac gccacgaaag gacacggcca cccgccatcg
    27661 acacgcgcga cgagaaagaa gcacggccgc gaatcgccgc ctcctactac ctcaactcgg
    27721 ccccgctcat ctggagcttc gcgcgcggcc cgcgccgcga gcaggtggag ttgctgaccg
    27781 acgcggcgcc ggcgcggtgc gcgcggatgc tcgcgcgcgg cgcggcggaa gcggcgctcg
    27841 tgcccgtcat cgagtgccag agtatcgagg gtgcggccgt tgtgcccggc gtctgcgtgg
    27901 gggcgcggga ggaagtgcag agcgtcgtgc tggtcacgcg cggggccggg cttgaggcgg
    27961 tgcgcagcgt cgcgctcgac accacgtcgc gcacttcggc cgcgctcgtg cggatcatct
    28021 tccgcgagtt cctgcgacgc gagccggagt acgtgccggc ggagccggac gttgaagcga
    28081 tgctcgaacg ctcggacgcc gcactcgtca tcggcgaccc ggccatgacg ttcgcgcgcg
    28141 cgggtctgcg cgtctacgac ctcgctcgac tgtggcgcga acacaccggc ctcggcttcg
    28201 tcttcgccgt ctggatggcg cgcgacgatg gggcgtcgcg tattcgtcag gtagacttcg
    28261 ccgccgcgcg cgacgagggc ctggcccgcg ccgaagagat cgccgccttc tacgagggcc
    28321 ggctcggacg gccacgcgcc gagctgctca agtacctgcg cgaaaacatc tgctacgagt
    28381 tgaacgaaga gatgcgccgg gggcttgagc ttttttacga actggcccat aagcataagt
    28441 tgattgaagg gttgaaacga ttgggtttcg ttgattcgta gttcgggaca gacgccggga
    28501 aaagcgatga aatatctctc agcagtaaaa gtactgatgg tggccttctt actctttact
    28561 ttccaaacga ttaagtcaca agaaatcgaa tacaaaggat gggataacgg aatcggtcac
    28621 tcctcttggg atataggttt aggaaaagag gagaaagaaa agatgcttca actttgggat
    28681 tccatcgggg aagatttaaa aacggagcaa aatgaactgg ccgggactta cctgaaggga
    28741 ggttatagcg ccggatattt ctttcgctgg tcaatcaaca agggttatgt tctgatccct
    28801 tatttcgacc agaacttaat aaccgatttc agctacggga aggtcacttt cgttgacagt
    28861 tcagaagttg tcttcacccc tgaaagagac ctgaaaggtg gtagaagcat tgggaagatg
    28921 cctcgtaaat ggacggccat tggtggatac ttcgttccgg tcgagatggt aaaagatttc
    28981 ggcgactaca tggctggatt cggggtatac aatgagttta acgggcagtg ctgtgccttc
    29041 gccccgaatt ttttagctca gagaattgat aggcaagaag agaaaatcga tcatcctgtc
    29101 tcgccgaaat atgcacggtt tattaagccc ccaatcaaag ctgacatcac gtttgtcgga
    29161 ataaaaaaga cggtcaagga ttggggttat gacggggagc tatacagtca atggatggaa
    29221 cgagcggttt taattcccgt taaaataagt gccggcagag cacacggcgt gaagcgcaat
    29281 atgcttttcc gtttaatcgg agaaccggat ttttatcagt atcttcagat aatgcgagtt
    29341 catcaacgaa cggcttcggg ctatatagtt cgtgatattt ctttcagagg gaaagaaacc
    29401 tatacggatt ctgatacaga ccaggaaaag ccgcttccgc ctattaaagt tggaacgaag
    29461 gtaacgacaa gtctcgttcg caactaagtc taacgtcaag ccttctgcta aaatcctttg
    29521 tagggattac ttgccatgaa agaaactaac attcaaccga ttttagataa ggcgttatcg
    29581 aacatccgcc tgacggcgga agactgcacg gcgcttctgg agtcgcacga catcgcgcgc
    29641 atcggcgtcg cggccgacga ggtgcgccgc cgtcgccacc ccgacggcgt ggtcacttac
    29701 atcatcgacc gcaacatcaa ctacacgaac gtctgcaacg tcgtctgcac cttctgcgcc
    29761 ttctaccgcc gccccggcaa gcccgacacc tacgtcctcg aaatcgacga ggtctgccgc
    29821 aagattgacg agaccatcgc gctcggcggc acgggcgtgc tgatgcaggg cggactccac
    29881 cccgacttcc gtctagagtg gtatgaagac ctcctgcgga cgctccacgc caaatacccc
    29941 gacttccagc tccactgttt ctcgccgcct gagattcaca acatccacct catctcgaag
    30001 ctcccttacg aggagatcat ggggcggctc aaggcagcgg gcctctactc cctgcccggc
    30061 ggcggcggag agattctgga cgacgaggtc cgcaagcgcg tctcgaccaa gtgtacgacc
    30121 gacgagtggc tcgacgtgat gcgcgccgtc caccgcgtgg gcctgcgctc gacggcgacg
    30181 atgatgttcg gcatcggcga ccgcacggag caccgcgtcc gacacctcca gcgcgtgcgc
    30241 gaccttcagg acgagaccgg cggcttcacg gccttcatcc catggacgtt ccagcgcgag
    30301 aacacggcgc tcggtcgtcg catcaaggat gagccgaccg ggatggacta cctcaagatg
    30361 ctctcggtct cgcgtctctt tctcgacaac gtcgagcaca tccagtcgtc ttggctgacg
    30421 caggggttgc gtctcgggca ggtggccctg cgcttcggcg ccgacgacat gggttcgatc
    30481 atgatcgagg agaacgtcgt ctcggcggcc ggcgcctcga atcacgccga cgaacagatg
    30541 ctccgctacc tgatccgcga ggcgggcttc cgcccgcaac agcgcgacat cctctatcgc
    30601 cacgtcaacc gcaacgacat gcccaacgat tcggacgcgg gcggctcggc gaagctgagc
    30661 gagctgacgg tcgcgttcgc ggattaagag cgcgtggcaa aactgtaggg gcagagcttg
    30721 tccgcggccc gctgcctcga aatcaaattt acatggatgg gcaggatgat caggataaga
    30781 gatttgaaat ttgaaatctc aaatctgtaa tcttaaatca gctctttctt catcctgcct
    30841 atcctgtcca tccatgtaaa tttctttcat cgttgagcgg gccgcggaca agccctgccc
    30901 ctacagtcat tatcgtcgtt gaggttttga aaccgacttt aagaaggcgt cgcgcgcgag
    30961 gggcgcgccc ttacggttac atcttgaaga tttccgtgta gtccttcacc agcttttcga
    31021 tcttcgcctt gaacgcggga tcgcgcatgg agacgctgac gtacttcacc tgtagcccgg
    31081 cgacgttcat ggcgtctttg gtgagcgtgt cggtcatctt ctgttgagtc tctttactca
    31141 cctgatagcc ccgcacgttt ttgagcgaac ccatcttcgc ctgaaggtcg gccttgcgtg
    31201 agtccatcag cttctgtgcc tcgtccacgc cggccgaggg gttcggggcc gactgcacct
    31261 tggacaccaa ctgttcggag aacgtgtgaa gctccgcgat gacggattcg atctccgcgt
    31321 ctttcttgca gccggcgagc gtaagcattg ccaaaacaag tacgaggggg acgcatttgt
    31381 ggcgcataaa ctttttctcc attcttaaga agcgctcagt gtgccgcgtt gtcgagcggc
    31441 gggcaggata ttacggacga ggcaggcatg ggccgcctcc gggcgaggtc gaatgcgaga
    31501 atgctgaacc gttgaacttc taccgcgcca gcgtttcctg agacttcgcg aggtcacggc
    31561 gcacggggct tgccggggcg gctcggaacg tttacaacat tttacatcaa cttcggtcgg
    31621 tcgccgttat aatttaatcc taacgcgata gagaatgagg tggttgaaac ggcgccccag
    31681 accttcggcc acgacccaca aacatttcaa accctcggtg gacacccgga atgaagaagg
    31741 agatgtcaga gatgagaagc attcgattca cgtcggtgtt gggagcggcc gtcatcatgt
    31801 gcgcggcagt cgccgcgtcg gcgcagacgg gcggcgagtg gccgcagtgg cgcggggcca
    31861 accgcgacgg cgtctcgaaa gagacggggt tgctcaagca gtggccggct gatgggccgg
    31921 cgctcgcgtg gaagggcgtt ggcgccggca tgggctactc gtcggtcgtc gtctcgaagg
    31981 gccgcctcta cacgctgggc ctgcgcggcg acaaggagta tgtcatcgcc ttcgacgccg
    32041 cgacgggcaa agaggtctgg gccacggcgc acggcgcggc cttccgcaac gaccgcggcg
    32101 acggcccgcg cggcacgccg accgtggacg gggagcgcct ctactcgctc ggcggcgccg
    32161 gcgacctctc ggccatcgac gccaagacgg gcaagatcgt ctggcagatg aacgtgctcc
    32221 agaagttcgg cggctcgaac atcacctggg gcatcagcga gtcgccgctc gtcatcggcg
    32281 acaaggtgct cgtcaacccc ggcggccccg gcgcgtcggt cgtcgcgctc aacaaaaaag
    32341 acggcacgct catctggaag agccagagcg accgcgccgg ctactcctca gccatccccg
    32401 tgcaggtggg cggcaagacg caggtcgtct tcttcacgca cacgcgcgcg ctcggtctcg
    32461 acgccgagac cgggaagctg ctgtgggact acccgaaggc ggccaacaac gtcgccaact
    32521 ccgcgacgcc ggtcgtgcgc ggcaaccgcg tctggatttc gtcggactac ggcacgggcg
    32581 gcggtgtcgt cgagatcaag gccgacggca cggccgccga ggtttatttc acgaaggaga
    32641 tgcgtaacca ccacagcacc tccattctca tgggcgacca cctctacggc ttctcggggg
    32701 gcatcctgac cgccatgaag ttcgacaccg gacaggtcgc ctggaaggat cgcagcgtcg
    32761 gcaaaggctc gctcgtctac gccgacggga tgctctacct gttcagcgag aacggcgtgg
    32821 tcggcctcgc cgaagccacc cccaccggct acaccgagaa gggccgcttc cgcatcccac
    32881 aagacaatct cccgacctgg gcgcacccgg ttgtcgccgg cgggcgtctc tacctgcgcg
    32941 accaggacac catctacgct tacgacgtga aggcgaagta gtagcgccgc gtgggcattc
    33001 gccgcaagtg taggtgaagt tcatgcgggg tcgcgcgcaa cgccagcgcc ggaggcgcgg
    33061 cagaatgtaa cccacggcgt gagccgtggg gataagtccg tttaatgaac cggaagcccc
    33121 ggaggggcga gagaacccca aggttgaccg ctatcaacct tggggttctt tcgcccctcc
    33181 ggggcttgtt gatttttatc ttgcccgtga cccacggctc acgccgtggg ctacattctt
    33241 tcgccgctcg cgcggctcgt tgatgcctgc gactgaatcg agttcaactc aacgcctcgt
    33301 gaacgttacg gcgaccggaa tggtcggaaa aattctgtca cgcgggaact gcgagggcgc
    33361 ggggcgggcg gtgaagcggt agaggccgcc gaagagttcg gtgaggcaac ggaggtagac
    33421 agtcgtcatt tcggcgggcg tgctcaagga cgctgagcgg atgatctggg cgatgcgcgc
    33481 gccgcgctgg agcgagcgcg cccagcggtt gcggcggtaa atctgctcca gctctcggag
    33541 acgcgcgggg tcgttgtatt cgagcatggc gcgcaggtag tagatggcgt ggagcgggtg
    33601 gtcgggcgag acgcccttgc cgcgcgtcca ggcgaggccg ccgatcatgt cggcgaccgt
    33661 gtcgaggccg agacggtgca ggtcgggcag gatggcgtgg tgctcgacgg cctccatcaa
    33721 agcgcccgtg agttccatct cgttcagctc gccgaggggc acgcggggcg acgagccggc
    33781 ctcgtcgaac atgaaggcgg ggaagccccg cgcgcgctgg cgcaggtgga gtatctcgtg
    33841 cgtgagggtc gcttcgagcc gcttcctgtt cgcggcagtc tggaagttga agttgatgcg
    33901 gatgaccgcc gcgccgcgct cggtgacgta gttctcgccg ccgacgaagc cctcgccccc
    33961 gtcctccggg ccgagctgcg cgaactcggc gtagacctcg cggcccagct ttcgctcaac
    34021 ctcttcaagc cacgcggcca cctccgggcg caacttgaga ccgagcagca cgcgctcgcg
    34081 cgcggggttc tgcccggcgg cagtttgcgg acggggctgt gcggcgggcg cgactacggc
    34141 gagagccagc gcgcagatga gcaaggccgt gcggcgaagg ttaatcatcc aacaccatca
    34201 cttttgatag acgtagtaga cgacgcccgc gtggtcgtcc gtcatcagga agccgttcgc
    34261 gccgaagcgc atgatgtcgc acgggcggcc gttgacgcgg cccgcgctct tgaagccgtc
    34321 gataaaagtc cgtgcggggt
//
LOCUS       DASVES010000143         5820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_25319, whole genome shotgun sequence.
ACCESSION   DASVES010000143 DASVES010000000
VERSION     DASVES010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25319"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..762
                     /locus_tag="VF240_05400"
     CDS             481..762
                     /locus_tag="VF240_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174701.1"
                     /translation="MSANKNDSTTGNTGTRDITYDLISTVYHLLQGAETVGMYIEDAS
                     QESDQEIVQFFRETKEEYTRRADEAKQLLTRHLGKDQGRAASGGGGGNQ"
     gene            complement(945..1265)
                     /locus_tag="VF240_05405"
     CDS             complement(945..1265)
                     /locus_tag="VF240_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7174702.1"
                     /translation="MEEITATELKQRIDSGDDLQIIDVREPNEYQTARIPGTTLIPLA
                     EVVGRRGEIDESRETVVHCKGGVRSAKAIEALRGAGFAGKLLNLKGGITAWSNDVDPS
                     VPKY"
     gene            complement(1368..2573)
                     /locus_tag="VF240_05410"
     CDS             complement(1368..2573)
                     /locus_tag="VF240_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX7174703.1"
                     /translation="MSEREEELEINTRVVHAGERLSAPVGSPVAPPIYATTTFTYDTM
                     AEMDEVFAGTREGYVYTRHGNPTVHALEEAVRALEGGAGACAYASGMAAMHAALLACN
                     LSPGSIVLASQDLYGATASLLKHVFGPFGVKTEQADFTNPDELAARVRQLRPRVLIAE
                     TISNPLLKVCDIEACAHAAREAGARLVIDNTFASPFLCRPVSMGADFVVHSATKYLGG
                     HGDATGGVVVTRDAGDTPALVSAMKLAGGILGVWDAHEILRGIKTLALRLERQCANAF
                     DLAERLTAHPRIARVRYPLLGDGAQRELAGRVLRRPHGGALVTVELRDATREAAFRFM
                     DALSLCVRSTSLGDVFTGVLHPATAAHRDLATERRLALGITEGTVRISVGVEDVGDIH
                     RDIVRALET"
     gene            complement(2662..3339)
                     /locus_tag="VF240_05415"
     CDS             complement(2662..3339)
                     /locus_tag="VF240_05415"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7174704.1"
                     /translation="MSTPAPTQEATSNAIPRINDTAPEFEAKSTQGPLKLSDYKGKWV
                     VLFSHPADFTPVCTTEFVEFARRSAEFKERDAQLVGLSVDSVTAHIAWIRNMEQHFGV
                     KVDFPVIADLDTKVAQKYGLLHPGASETATVRAVFVIDPNQKVRALVYYPMSLGRNID
                     EVLRVLDGLQTADQNACATPANWQKGDKVIVPPPQTLADAEQRMTTPGYEVTDWYFSK
                     RDLNEGK"
     gene            3875..4600
                     /locus_tag="VF240_05420"
     CDS             3875..4600
                     /locus_tag="VF240_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174705.1"
                     /translation="MIRNNRLAQRAFSIFALVALVAATMIPAFGATQRRQRRPVRRAV
                     TRRATPPRVVLHTVPADTVIRVRMNSQLKSDSARVGDRFSANVTELVYGGNGVEVIPT
                     GSKVWGSVTSVRRAGRRTPGSISVSFNQVELPNGARYAINGSLSSLQADDVNSDNEGT
                     VSGRSNRKRDAVFIGGGAAAGAIIGAIAGGGRGAGIGAILGGGAGTAGRVYEREQDAE
                     VKSGTEFGVILNRSISLPRFGDR"
     gene            complement(5085..>5820)
                     /locus_tag="VF240_05425"
     CDS             complement(5085..>5820)
                     /locus_tag="VF240_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7174706.1"
                     /translation="EGDWSLRLGLRGMPALAEESDVARDCALAELTLARLHIAHVSTR
                     GAIEAVRRAKARGLSVTCEVAPHHWTLTDEAAAEFDTNSKMSPPLRSPEHVEAILEAL
                     RDGTIDAVATDHAPHHADEKALEFDHAPFGITGLETAVGLALERLVHRGVVDLERLVA
                     LCSANPARILNLEGRGTLRPGSWGDVTILDPDLRWTFDAARSKSKSRNTPFDGWTFQG
                     AAVATIVAGRVVYRREQSAVGSQQSA"
BASE COUNT         1005 a         1812 c         1943 g         1060 t
ORIGIN      
        1 tggtgtccat atgaggagag gatcaagcgc cttcaagaac ttggtgcaag aattcaagcg
       61 ggttgtgaaa agcatcctct ggatggttat ctgcgccaat tgttggaaga cattaagcga
      121 gcagaactat atacgactac atcctggtaa actgaattcc acaaaataac tcttttgtgg
      181 aattcagaag gacatagcac gggcagttat cttgaccatg ctcctctgaa gtttaaagaa
      241 agcctggcat ttctgtaatt cggaagtgcc gggcgagtta gttttagtat agtcggcgac
      301 aggtgactcc cgctgtaatg ttgaatttgc ggaaattcct ccgctggctg tgcgggtaaa
      361 tgcccgcgcg gcgcgacgcg gccttgaccc gcggcatgcg ccgttcctac aatccgtccg
      421 aaccaagttc ccaaacggcc cccgtcgctt cgcaacttac attctccaaa ggggtgaact
      481 atgtctgcta ataaaaacga cagcacgacc ggcaacaccg gaacccgcga catcacttac
      541 gatctcatca gcaccgtcta ccacctgctt cagggcgccg agaccgtcgg catgtacatc
      601 gaggacgcca gccaggagag cgatcaggag atcgtccagt tcttccgcga gaccaaagag
      661 gagtacacgc gccgggccga cgaggccaag caactgctca cgcgtcacct cggcaaggat
      721 cagggccgcg cggcgagcgg tggcggcggc gggaatcagt aaacactttc gggacgccgt
      781 cgctcgcggc ggcgctctcg tcggagaact tcaaaatcag aaacgcctgc cgaaacaacg
      841 gcaggcgttt ctgatttaaa gccgcgacgg ccgcagactc agcggcgcgc ctcgcgagac
      901 ggccgacgcg cgtcactgtg gagccgcgtt gagtgtcggc gtgctcagta cttggggacg
      961 ctcggatcga catcgttcga ccaggcggtg atgcccccct tcaggttcag cagttttccc
     1021 gcgaagcccg cgcccctgag cgcctcgatg gctttggcgc tgcggacgcc gcccttgcag
     1081 tggacgaccg tctcgcgcga ctcgtcgatc tcccccctgc ggccgacgac ctccgcaagc
     1141 ggaatcaagg tcgtgccggg gatgcgcgcg gtctggtact cgttcggctc gcgcacgtcg
     1201 atgatttgca ggtcgtcccc gctgtcgatg cgctgcttta actccgtggc ggtgatctct
     1261 tccaacttac gttcgcctcc ttcatttggt tttcagtcgg tagtgcgtga ttctcggcgg
     1321 tcttcatcag ccggtctcac atccctgccc ttcggcaaac ttactcttca cgtctcaagc
     1381 gcccgcacga tgtctcggtg gatgtctcca acgtcctcga cgccgacgga gatacgcacc
     1441 gtgccctcgg tgatgccgag cgcgaggcgg cgctctgtcg cgaggtcgcg gtgcgcggcc
     1501 gtcgccgggt ggagcacgcc ggtgaacacg tcgccgaggc tcgtcgaacg gacgcagaga
     1561 gagagcgcgt ccataaagcg gaaggcggcc tcgcgcgtcg cgtcgcgcag ctcgacggtg
     1621 acgagggcgc cgccgtgcgg gcggcggagc acgcgcccgg cgagttccct ttgcgcgccg
     1681 tcgccgagca gcgggtaacg gacgcgcgcg atgcgcgggt gcgcggtgag gcgttcggcc
     1741 agatcgaagg cgttcgcgca ctggcgttcg agacgcagcg cgagggtctt tatcccgcgc
     1801 agaatttcgt gcgcgtccca cacgccgagg atgccgcccg cgagcttcat cgcactgacg
     1861 agcgccggcg tgtcgccggc gtcgcgggtc acgaccacac cgcccgtcgc gtcgccgtgc
     1921 ccgcccagat atttcgttgc gctatgcacg acgaagtcgg cgcccattga aacgggtcgg
     1981 cagaggaagg gcgaggcgaa ggtgttgtcg atgacgagtc tcgccccggc ctcgcgcgcc
     2041 gcgtgcgcgc acgcctcgat gtcgcagacc ttgaggagcg ggttcgagat cgtctcggcg
     2101 atgagcacgc ggggccggag ctgcctcacc cgcgccgcga gttcgtcggg gttggtgaag
     2161 tccgcctgct cagtcttcac gccgaagggg ccgaagacgt gtttgagcag gcttgccgtg
     2221 gctccgtaga ggtcttgcga ggcgaggaca atcgagccgg gggagaggtt gcaggcgagg
     2281 agcgccgcgt gcatcgcggc catgccggag gcgtaggcgc aggcgcccgc gccgccttcg
     2341 agcgcgcgca ccgcctcttc gagcgcgtgc acggtcgggt tgccgtggcg tgtgtagacg
     2401 tagccctcgc gcgtgccggc gaagacctcg tccatctcgg ccatcgtgtc gtaggtgaag
     2461 gtcgtcgtgg cgtagatggg cggggcgacg gggctgccga cgggcgcgct cagacgctcg
     2521 cccgcgtgaa cgacgcgcgt gttgatctcc aactcctctt cgcgctcaga catccgttcg
     2581 tgtcccgccg ccgcttcaga ggccgcgccc gaattcatac cggggcgcgg ccgtcgatta
     2641 acgaccgcta cgccgcccgg cctacttgcc ctcgttcaaa tcccgcttgg agaagtacca
     2701 gtcggtgact tcgtagccgg gcgtggtcat gcgttgctcg gcgtcggcga gcgtctgcgg
     2761 cgggggcacg atcaccttgt cgcccttctg ccagttggcg ggcgtggcgc aggcgttctg
     2821 gtcggcggtc tgaagaccgt ccaagacgcg cagcacctcg tcgatgtttc tgccgagcga
     2881 catcgggtaa tagacgagcg cgcgcacttt ctggttgggg tcgatgacga agacggcgcg
     2941 caccgtggcc gtctccgacg cgccggggtg aaggagtccg tacttctgcg ccaccttcgt
     3001 gtcgaggtcg gcgatgacgg ggaagtccac cttcacgccg aagtgttgct ccatgtttct
     3061 aatccaggcg atgtgcgcgg tgacggaatc gacggagagg ccgacgagct gcgcgtcgcg
     3121 ttctttgaac tccgcggagc gccgggcgaa ctcgacgaac tccgtcgtgc agaccggcgt
     3181 gaagtcggcc ggatgcgaga acaggacgac ccacttgccc ttgtagtccg agagcttgag
     3241 cggcccctgt gtggacttgg cctcgaactc gggcgccgtg tcgttgatgc gtgggattgc
     3301 gttcgaggtg gcttcctgcg tcggtgctgg tgtgctcatc agaagaactc ctgttcgttt
     3361 ggggtggaaa gtttctttga gacctatggc catttgcaga tgggtgcgtc cgtccgtgcg
     3421 ccgggcggag ggaacacgga tgagacggat gggacggatt aagaaggatc gaatccgtgc
     3481 ccatccgtcc ttttccgttt aatctgtgtt cctctggttt gaggcccaag cttacaacaa
     3541 aaccgcttcg atagcccaac gcgaaatgag catcgcgcga aggcgcgcgg gtctgctccg
     3601 tgtcatccga cggcccctct aatcaccctc gcaccatata gtgcatgtgc ttgcacaacc
     3661 tgcttcgtat agtcgccgga tcgccggccg cgcgccgtcg ccgcttgcgc gcccggctct
     3721 aaatcaagta atagaatgtc ttagcaaatt tcacctcgac atgccccgcg gtggcacact
     3781 cgttgccaaa gcataatcgg cgacggttct gttgcgccgg gccggctggc cgcgaggcgt
     3841 cagcaagaac gtcaacaacg gagggagaca gttaatgatt agaaacaata gattggcaca
     3901 acgcgccttc agcatctttg cactcgtggc gctcgtcgcc gcgaccatga tcccggcctt
     3961 cggcgccacg cagcgccgcc agcggcgtcc cgttcgccgg gcggtaacac gcagggcgac
     4021 gccgccacgc gtggttcttc acacggttcc ggccgacaca gtcatccgcg tgcggatgaa
     4081 ctcgcaattg aagtctgact cggcgcgcgt gggcgaccgc ttctcggcga acgtcaccga
     4141 gttggtctac ggcggcaacg gcgtcgaagt tattcctacc ggaagcaagg tctggggcag
     4201 cgtgacttcc gtccgtcgcg ccgggcgacg cacgccgggc agcatctccg tgagcttcaa
     4261 tcaggttgag ttgcccaacg gcgcccgcta cgcgatcaac ggctcactct ctagccttca
     4321 ggccgacgac gtgaactccg acaacgaggg cacggtctca ggcaggagca accgcaagcg
     4381 cgacgcggtc ttcatcggcg gcggcgcggc ggccggcgcc atcatcggcg cgattgccgg
     4441 cggcggcagg ggcgcaggta tcggggccat cctcggcggc ggggccggca ccgcgggccg
     4501 cgtctacgag agggagcagg atgccgaggt gaagtcgggc acggagttcg gcgtcatcct
     4561 caaccgcagc atctcgctgc cgcgcttcgg cgaccgatag atatttcaag gatcggcggc
     4621 cgcgaggtcg ccgtgcaacg ggggcctgca agcgaagggg agagcgcggg caggcccccg
     4681 ataaaaaatc gataaagggt tcggtcgccc ctgtcaggac gtaagtgagc gaccgcaacg
     4741 agggaggccc gccgcgaagg ggagagcgcg gcgggcctct ctgcgtttgg cttttagctt
     4801 tcagctttta gctttcagct tttcggattc gacaaagcgc gcgcaggatg gggcgacgac
     4861 gaccagcccc gtcaggggcg gaagaatgtt gccacgggcg caagcccgtg gaacagcggt
     4921 gagaaaaatc gggaagcccc ggaggggcga aagaatcggg acaggcgcca cgcccaacat
     4981 taagattctt tcgcccctcc ggggcttcgc attgtttcgc cgcctgtccc caaggctcac
     5041 gccgtgggca acactgctgc cgccgctccg cggctcgttg agctttatgc tgactgctga
     5101 ctgccgactg ctgactgctc tcttctgtaa acgacgcggc cggcgacaat cgtcgccacg
     5161 gccgcgcctt gaaacgtcca gccgtcgaag ggcgtgttgc ggcttttgga tttggaacgc
     5221 gcggcgtcga acgtccagcg caggtcgggg tcgaggatag tcacgtcgcc ccacgagccg
     5281 gggcgcaggg ttccgcggcc ttcgaggttg aggatgcgtg cggggttggc ggagcagagc
     5341 gcgacgagac gttcgagatc gacgacgccg cggtggacga ggcgttcgag ggcgaggccg
     5401 acggccgtct ccagccccgt gatgccgaag ggagcatggt cgaactccaa cgccttttcg
     5461 tcggcgtggt gcggcgcatg gtcggtggcg acggcgtcga tagtgccgtc gcgcagggct
     5521 tcgaggatgg cctccacgtg ctcggggctg cgaagcggcg gactcatctt gctgttggtg
     5581 tcgaattcgg ccgcggcctc atccgtgagc gtccagtgat ggggcgcgac ctcgcaggtc
     5641 acgctcagtc ccctcgcctt cgcgcgcctg acggcctcaa tggccccgcg cgtcgagacg
     5701 tgtgcgatgt ggagtcgcgc gagcgtcaac tcggcgagcg cacagtcgcg cgccacgtcc
     5761 gactcttcgg cgagcgccgg catcccgcgc aggccgagac gcagagacca gtcgccctcg
//
LOCUS       DASVES010000144        32917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1388060, whole genome shotgun sequence.
ACCESSION   DASVES010000144 DASVES010000000
VERSION     DASVES010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 32917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32917
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388060"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2835..2939
                     /locus_tag="VF240_05430"
                     /pseudo
     CDS             2835..2939
                     /locus_tag="VF240_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            2952..3500
                     /locus_tag="VF240_05435"
                     /pseudo
     CDS             2952..3500
                     /locus_tag="VF240_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            3501..3818
                     /locus_tag="VF240_05440"
     CDS             3501..3818
                     /locus_tag="VF240_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX7174707.1"
                     /translation="MPDTADNCDFTANPDQADADGDGIGNACESDDDNDGVPDTADNC
                     PLTPNPNQADFDGDGIGDTCDPQTGPPVNKNQCKNGGWMRFDVPRTFKDQSDCIQYVN
                     TGK"
     gene            4251..7412
                     /locus_tag="VF240_05445"
     CDS             4251..7412
                     /locus_tag="VF240_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7174708.1"
                     /translation="MFKFFRLPLAALFVIATTCGAAFAQSTTDGAIGGVVRDPNGAVV
                     VNAPVTVRNEETNKETAATTDDEGRFRVVQLQPGSYTVTVNATGFGPFTAQKVVVEVG
                     RVTPLEATLSVTGTSETVEVTSEAPVINTERQDLSTNINQTSINNLPINGRRWSNFAL
                     LTPGAVPDGSFGLVSFRGVSGLLNNNTVDGGDNNQAFFSEERGRTRLSYSISQDSVRE
                     FQVNTSNYSAEYGRAAGGVVNAVTKSGTNEFHGNAFFYVRDDKFNARNPQLATKTEDN
                     RKQFGGTIGGPIVKDRAFFFFSYDQQKRIFPGISTYSTNTFSTANFNNANLLLRGLTQ
                     AQIDATRSFLDSLTGPFDRKGDQTIFLPKLDLRINDSNALAVTYNRLRWKSPNGVQTT
                     SSLTRATTSIGDDLVDVDWITLRLTSTISSNLINEARFQYGRDFERQTYPGPLPGEPT
                     TAPPGGVPPQIGLPGYTIGKPNFLDRRSFPDEKRWQYADTVTALVGNHTFKFGVDINH
                     VNDINDNLFTESGSYVYSTTSDFFVDYANFLSNNALRALSATTATTNPLGRCSGSTRR
                     AGQCYNGTFNQGFGTPKFQFETDDYNFFIQDDWRITPRLSINLGLRYEYQKLPAPQIP
                     NATFDSDPRFTAKTGVFPADKNNFGPRFGFAYNITGDSRNVVRGGYGIYYGRIQNSVV
                     SNAITNTGNPAGQTQLTLTTAIFPQAFQTAADASGGTAPIPNIVAFASNMANPQIHQA
                     DLIFEHELTRNTVVSASYIMSAGRRLPTFVDVNLAFPTSTTAIPFSGGPFDGQTLRVP
                     RFTTRINNAFNAITEIRSTIKSEYNALVLQFNRRYTNGLQFQTNYTFSKAKDNGQATG
                     TFTNTNAPLSPYDYSLEVGPSNFDIRHRFVASAVWTPDFFGEDQKVGRAIFNGFNIAP
                     VFVLQSGFPYSMGISGTPTAAAFGPTIVRGGSNLTGTTATGRVPFLGRNSLRFPRFAN
                     FDLRVSRRFRITETMNFEVLAEAFNIFNRTQVIGNLDTTAYTINNTTNNLIFQPTFQS
                     IQPPAGETLYKARQMQFALRFEF"
     gene            7722..7934
                     /locus_tag="VF240_05450"
     CDS             7722..7934
                     /locus_tag="VF240_05450"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX7174709.1"
                     /translation="MGFPNKDEVEGKFDQAKGAIKENVGDAIDDEKMQSEGAAERAGG
                     HAQEGFGTARRKVGETIEDIGNKVGG"
     gene            8253..9812
                     /locus_tag="VF240_05455"
     CDS             8253..9812
                     /locus_tag="VF240_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7174710.1"
                     /translation="MKIESRRLALPLLLVAIPLVAFVAFVTWRVVTREAPPDAQEPVD
                     EIIRSTDVPMVEPVARDLAAIKEAGTLTVLAPYNSTTYFLYRGEPMGYEYELLRAFAQ
                     EHGLALKMVVVTDRKSLYSLLNSGEGDIAAARIVPTETDRAHVAFTRELYRTEPALVQ
                     QEQPPAEANLSHAAERALQPAPDAPLPEDLEIQARLITRPAQLAGETVTLPEKSTYKR
                     TLVELADEISGEIRVVEMGGKMEDEALARQVASGEVEFTVMQRNVAELKEAEFTNLKV
                     RPVVGRVHSVSWAVRPNSPALLEELNRWIAEKKSQALLATLYKKYFIDVKKHRERAAS
                     EYLTSSTGKLCEFDTLLKRYAGELSWDWRLLASQAFQESRFKPDARSWAGATGLLQLM
                     PRTAREFGVQDMNNPEQNVQGAVRFLRWLTDYWTKRIPDEQERLKFILASYNTGAGHV
                     EDAQRLTEKYGGDKLKWDDVSYWLLQKSTQQYAADPVVKFGYCRGLEPVTYVERILER
                     FEHYRQFVVDA"
     gene            complement(9900..10751)
                     /locus_tag="VF240_05460"
     CDS             complement(9900..10751)
                     /locus_tag="VF240_05460"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7174711.1"
                     /translation="MERAERFVEIDAPVEVVFDRLSDFESFPRWMRNIREVRRLGRRV
                     TRWVSETAVPDVYAEWEAELTVYEPDHRLVWRSVRGDIHADGEAVISETPEGTTLLHV
                     VLGFGTPLGRSGAQAARFFGVQPGRQLEEDLERFRRLAEREGRGRQGYAGEGRRSSFA
                     PVMSRREEPRRGAREERRPLRTREEGRVRDDAPPPRRDERMTRRRDYEDAPRRDEERI
                     WRAPEREGGDKGSRPRYALTPRERERERDDYERRYDPRVSEMFGRRGVDRLLDEPPDG
                     RRRGGRE"
     gene            complement(10850..11350)
                     /locus_tag="VF240_05465"
     CDS             complement(10850..11350)
                     /locus_tag="VF240_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX7174712.1"
                     /translation="MTKLVTGVFKTRVAAEAAVDAIIKRGYTREDISVLMSDATKTKE
                     FAIETRTHAADGAGIGGAVGGAVGAVLAALAAVGTTMVIPGIGIVVAGPLAAALAGMG
                     AGGATGGLIGALVGAGIPEHRAKVYDASLRAGGILLGVESKSDEDAGALEKLLEDLGA
                     EYIRQE"
     gene            11748..12425
                     /locus_tag="VF240_05470"
     CDS             11748..12425
                     /locus_tag="VF240_05470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7174713.1"
                     /translation="MIKAVIFDIDGTLVDSVDLHAKAWREAFRHFGREFPFERVRYQI
                     GKGGDQLMPVFLSPDELERFGDELEKHRGELFKKEYMPRVRPFPRVRELFERIRADGR
                     RIALASSAKEDEVKVYRKIADIGDLIEEQTSADDAERSKPYPDIFEAALERLDDVGPK
                     ESLVVGDTPYDAQAAGKIGLRTIGLLCGGFPEEDLRAAGCVEIYKDPADLLARYEESL
                     IAVNRQS"
     gene            12544..13662
                     /locus_tag="VF240_05475"
     CDS             12544..13662
                     /locus_tag="VF240_05475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7174714.1"
                     /translation="MRILQISSARTLGGGERHFADLANGLARRGHEVYAALAPGSPLR
                     DELRDLPADNVLTLSLRNALDLKSASGLARLARERRVELIHAHVARDYTLAAFAARRA
                     RGARFVVTRHVLFPLGHAHRIVLARAARVIAVSEAVARTLRARGVFPEHKIRVVQNGI
                     DIERLERAASKLDCGTYRRELGVRAPQMVGSVGELSEVKGQADLVRAAVAVLREMGNA
                     VEFVIVGEDASRGGGNRAKLEASISELGVGGHVRLAGRRGDVAEFMSCLDVFVNTSRS
                     ESFGLATVEAMACGAAVVATATDGSRELIEDGVTGLLTPVGDAGALAAAVLRLLRDES
                     LRASLGDRARRLARERWSVERMIDETERVYLEALSQDA"
     gene            complement(13746..14069)
                     /locus_tag="VF240_05480"
     CDS             complement(13746..14069)
                     /locus_tag="VF240_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174715.1"
                     /translation="MKTKKQTLKSQTFTPAEQRAARSERVVVTDAEPYGWMAHSESHA
                     EQNYHLFRNPESKRLVCTCADFIFRGDAEPTYECKHVSAVLKFIARRYLRQEYDPRQQ
                     YRNAA"
     gene            14357..15169
                     /locus_tag="VF240_05485"
     CDS             14357..15169
                     /locus_tag="VF240_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7174716.1"
                     /translation="MSATTRAVRGLEMTYNVRGGGAPVVLLHGFPFNHTMWRGQVEAL
                     SEGHRVITPDLRGHGGTGLVEGTTSSSMEEMATDVAALLDELNVSERVVLGGLSMGGY
                     VTLAFFRLFADRVRALVLADTRPQPDTEEARRAREETAQRALREGMQTVADAMLPKLL
                     APSTHEEKPEVIERVREMILATRPEGAAAALRGMAARRDQTDLLQEIVVPTLIIVGSE
                     DSLTPPKDSEAMHHAIRGSRLVIIEGAGHVSNLERPTEFNRALEDFLNGIES"
     gene            15187..15630
                     /locus_tag="VF240_05490"
     CDS             15187..15630
                     /locus_tag="VF240_05490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7174717.1"
                     /translation="MNETWKYRIEERVRWGDVDAARIIFYGAYIRFFEFAETELFRAV
                     GLPYGVMFDELDVWLPRAHLECDFRRAAQLDDLLEVGVYVGRIGNKSLRLNFEVRRKG
                     EGEMLAEAHFVLVAVRRDTFETVTVPDELRTRLAPYTQPLKRALS"
     gene            complement(15670..16899)
                     /locus_tag="VF240_05495"
     CDS             complement(15670..16899)
                     /locus_tag="VF240_05495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7174718.1"
                     /translation="MANHTKYDMLIVGAGPAGSFAAERLARGGARVALFDGRPHTEAK
                     ACGGGVTSKALKAWPFLLEAAARTVDEVDLHSPAGRRVRLKLQDPFAIYSRRVFDSHL
                     LERARIAGAEVLPVRVSATPCEETEGGWVARARDGRAWSGRVIVAADGANSPIARRLA
                     GPLPNAEMEVAFGYRTPLDEGADAPTVIAFLPGWAGYAWAFPRLDHISFGIATTQVSF
                     DHRALDRLLWDFMVGYYRTRDDETARVWTAGEGVARGGERDRSIEAELRRTAERYAAR
                     IPGLTPETLERRRASGKDWALLGDAAGFADPVTGEGIYYALRSAELFAEAWLEGRAEA
                     YDELWRGDFGRELRRASQMRGRFYGTFMGATFTDRMVEFARAHRGVRRTLCELIAGEQ
                     GYVNLKRKLVRRAFRPV"
     gene            17138..17959
                     /gene="amrB"
                     /locus_tag="VF240_05500"
     CDS             17138..17959
                     /gene="amrB"
                     /locus_tag="VF240_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX7174719.1"
                     /translation="MATGSMVFVGIAPHPPIMIPEVGGEIVSEVRGSVEAMEELTRRI
                     SESGAESVVIISPHAPLEARAFVAYGGPTLRGDFAQFRAPQAIIDMPLDGDLLTAIAR
                     EAADEGLHVMPLEGYELDHGTAVPLYFLRRFGWDGRVVALGYSFLTDEDHIRFGACVR
                     RAVDAEGRAVAFVASGDLSHRLKPGAPAGYNPDARLFDEQVVAAISSSDPLGIARVDQ
                     GLRRLAGECGYRSMLVALGTLAGAPINCEVLHYEAPFGVGYLVAQLLRGGDMGEK"
     gene            17956..18588
                     /gene="amrA"
                     /locus_tag="VF240_05505"
     CDS             17956..18588
                     /gene="amrA"
                     /locus_tag="VF240_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX7174720.1"
                     /translation="MSGDDKRVDETYVPPPSSGDAELTSPELSDETKIPALARLAVET
                     FIRERRLLDSDCAPELPVLRQPAACFVSIKTGRNDLRGCIGTIEPSRRTLAEELIINA
                     VHAATRDPRFMPVDEHELPSLRYSVDVLSEPEPTEFESLDPSLYGVIVEDQTGTRRGL
                     LLPDIEGVETSTQQVQIAARKAGIDPDEPLRLFRFQVTRFRESGKHTSSG"
     gene            complement(18758..21205)
                     /gene="gyrB"
                     /locus_tag="VF240_05510"
     CDS             complement(18758..21205)
                     /gene="gyrB"
                     /locus_tag="VF240_05510"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX7174721.1"
                     /translation="MSTVTIPKKKSTNYDSTSITVLEGREAVRKRPAMYIGSTGELGL
                     HHLVYEVVDNSIDEALAGYCDTVEVTIHLDNSITVIDNGRGIPVDYHKQERRSAAEVV
                     MTKLHAGGKFDSNAYKVSGGLHGVGVSCVNFLSELLSLEIWRDGQTHEQEYKRGIPVA
                     ALKVTGKTRKRGTKITFKPDSEIFDVSEFSFDKLSERLREKAFLNKGIRITIRDEREE
                     PERSHEFYYRGGIAEFVKHLNKNKSILHDKPLYFERIGDALSVEVAIQYNDAYDEKVY
                     SFANNINTVDGGSHLSGFRSAITRTINAYAQSSGLAKNFKSSLSGDDVREGLVAVISV
                     KLPQPQFEGQTKGKLNSDVKGPVESFLNEKLTEYFEQNPAVAKKIVGKSIDAARAREA
                     ARKAREIVRKGAIGSSMLPGKLADCQERDPALCELYIVEGDSAGGSAKQGRDRRNQAV
                     LPLKGKILNVEKARFDKMLSHSEIKTLITALGTGIGKDDFDIGKLRYHKVVFMTDADV
                     DGSHIRTLLLTFFYRQMPELLESGHIYIAQPPLFKVKRGKREEYIKDERSMVRYLMRQ
                     ATSDLKVTSSSAKTVTEGRELARSLEQLVELNRYCERAARRLGNDARLLHALLEALGG
                     RQGVLRNEGNPTLRSVFQDGEGEQMATVEGALAEAGYRTDLTMDEEHGLWAIETTSAG
                     GFKVLIDWDLASHVEFQKAVDLYKTLEDRLAAPFVLGENGSSEQIESREVLLERVLAT
                     AKKDLTIQRYKGLGEMNPEQLWETTMNPEKRTLLQVKIDDAVETDQIFTVLMGDAVEP
                     RRRFIEDNALDVKNLDV"
     gene            complement(21287..22438)
                     /gene="recF"
                     /locus_tag="VF240_05515"
     CDS             complement(21287..22438)
                     /gene="recF"
                     /locus_tag="VF240_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX7174722.1"
                     /translation="MLLEFLEVHNFRNLSGKVNWGPGLNIIYGENGQGKTNWLEAIYL
                     LSTSKSFRTQRLQDAINFGEDLAIVRGRVTRTQDVQRDLQVTLQGNSKTLSVNAKREA
                     VASYLSQLHTVAFTAEEMEVVRGGPEARRRFLDRGVISLHHAYVQTLSDYQRVIKQKN
                     RLLQDASDAELAFDEARKLIEPWNEQLVKLSAIIHRSRNEYVERLNGVLESRFFGREE
                     VSIRYLSSLEGKGDLSDYEALIAERLKLRLPAEIASGYALIGTHRDDLQILFDGRDMR
                     AFGSSGQQRSALILLDLAAISVYHSWHSEYPIFIMDDVDAELDRMRLDRLLESLEGRT
                     QTFVTTSKDDFVKRFAARSNVYEVSAGAAGERMSEAELISSIATDVNGI"
     gene            complement(22514..23626)
                     /gene="dnaN"
                     /locus_tag="VF240_05520"
     CDS             complement(22514..23626)
                     /gene="dnaN"
                     /locus_tag="VF240_05520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7174723.1"
                     /translation="MEFTISKNALQKELGFVQGIIERKNTIPVLSNILIESVGENAIR
                     FLGTDLDVTLRCETEADIKSPGSMCVSARKLFEIARVLPDAPVNFRKEENNWAAVTCE
                     RSNFRVAGVERESFPEVPSFVIPPVKLSGSIFKTFIDRTIFAITLEESRYTLSGAKFI
                     LNQSGAKMITTDGHRLAYIERKDLGETAPKENLDVLIPRKTLAELTKLMSGNEGDVGI
                     GADENHIYFEVGPRLLISRTLAGQFPNYDMVMPKGNDRKAEFDSTLLGQAIRRVALMA
                     DDRSHAIRLHLMPGTLQISSQTAEEGEARESIATEYAGEETEIGFNAAYLQDFLSVVN
                     GGSVVFEFKDGNSQAQLRLAPDDGYDYRYVVMPMRL"
     gene            complement(24007..25464)
                     /gene="dnaA"
                     /locus_tag="VF240_05525"
     CDS             complement(24007..25464)
                     /gene="dnaA"
                     /locus_tag="VF240_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7174724.1"
                     /translation="MSTMFESQVWNNILHAVEKRLNRQSFETWIRPIHFEGYDETEHK
                     LHLRAPNQVVKDWVSTNYSDVFSASLEELNLSSYEVDWTVQESETSKSFGGDEVKDNF
                     GLNNNPDQGAQKDRSSNLFSLLEAAETDGLTPGATTFVDIEPLENSLNPKCSFESFVV
                     GSCNQFAHAAALAVAEAPGKTYNPLYVYGGVGLGKTHLMHAIGHKIKTNNRYLRLSYI
                     SAEKFMNELINAIRYDKTQTFREKYRSIDVLLIDDIQFMAGKERTQEEFFHTFNALYD
                     AQKQIVITSDCPPREIPTLEERLHSRFEWGLIADIEPPDLETKVAILKRKADLDGVSL
                     PDDVAFFIASKVKSNIRELEGSLVRLIAISSLRGLPISKMLAQDAIRNIADDNQPAGI
                     TIEQIQRVVSAHYRLRADDIKTKNNSRQIAVPRQVAMYLCKRLTKHSFPEIGREFGGK
                     HHTTVIHSVDKIEALITRDQNFHRVVSDLMDNLCK"
     gene            26024..26170
                     /gene="rpmH"
                     /locus_tag="VF240_05530"
     CDS             26024..26170
                     /gene="rpmH"
                     /locus_tag="VF240_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7174725.1"
                     /translation="MPKRTFQPNNRRRAKTHGFRARMATKNGRLVIKRRRAKGRKRLT
                     PSHY"
     gene            26209..26541
                     /gene="rnpA"
                     /locus_tag="VF240_05535"
                     /pseudo
     CDS             26209..26541
                     /gene="rnpA"
                     /locus_tag="VF240_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
     gene            26559..26768
                     /gene="yidD"
                     /locus_tag="VF240_05540"
     CDS             26559..26768
                     /gene="yidD"
                     /locus_tag="VF240_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7174726.1"
                     /translation="MKAIILGLLRFYKAAVSPLLPPSCRFIPTCSEYAREAVERHGAL
                     RGTALGARRLLRCHPFHPGGYDPVE"
     gene            26830..28752
                     /gene="yidC"
                     /locus_tag="VF240_05545"
     CDS             26830..28752
                     /gene="yidC"
                     /locus_tag="VF240_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX7174727.1"
                     /translation="MEQKRLFLALIISTVILLGWSYFFQPSTPQRNSNASQAPPAESP
                     QASNQQPDTAPRPAEPSLTPGGDVKVSEDTVRPQVIVVSTPLYKVEIDNRGAVVKSWI
                     IRQNKDTGRPLYSIAGDKEKRIDLELVSPEGLSRGQAPLSLEIARLGLNDGSTPQLLP
                     ALNDALKNRYFEVTGRNFALGTASAEGGPINLNIESGEPKKYLDFKMVEPDTGLEVVK
                     SLAFDPQSYTTDVEVKVNRRGQTVPDVRMLIGPSTGDQGVPLYTFYSVAPQGVAVAKG
                     DAVRIYATDVHKQKSAQDPVKGYKPIEGPVDWAAVGDTYFAMALVLPRPAEKVEFLTE
                     EYQHTSGDTKEQRFLISGLVPVPTDGSAMSLYVGPKDHFALDDTSRTLSQVAGREVDL
                     EQLIDYGWLGFTRPIAVQLLKAIKFLNDVTGNYGVAIILFTIIIYMLFFPLKWRSTKS
                     MKKAQKHAPRMKEIQQKIKELKPNDPRLKELQMEQLRLMKEANPLGGCLPLLIQMPFL
                     IALYSAITISIDFRQASFLWMPDLSASDPIRLLPILMAGSMVILQFITPAPSADPLQR
                     KMMAVVLPVMMLYMLWSAPSGLLVYWLVGNIVGFGQQFVINRMVKSEDDEEPPGKKKE
                     VNPKKPKLGATSVSQA"
     gene            28791..29255
                     /locus_tag="VF240_05550"
     CDS             28791..29255
                     /locus_tag="VF240_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX7174728.1"
                     /translation="MKEIFNQSQDFLNQVFTSVRLDLQASAAETEGGCVLNIDGADAA
                     LLRSEGGELLDALEHLVNQSFARGLEPGERLVCDVEDFRLVRETELRAMARHAADRVR
                     ASGVPFMFGPMNANERRIIHLELANEEQLHTESVGEGSDRRLKVSLKSPSRQ"
     gene            29309..30676
                     /gene="mnmE"
                     /locus_tag="VF240_05555"
     CDS             29309..30676
                     /gene="mnmE"
                     /locus_tag="VF240_05555"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX7174729.1"
                     /translation="MQATDTIVALSTPPGRSGIGVIRLSGASSLDLLKILVRDQHFAP
                     EPNRVTLKGIYVPDRGELLDRALITYFKSPHSFTGEDVVELSCHGSPPLLLHVIDALL
                     SSGARTADPGEFTLRALSNGRMNLCQAEAVRDLIDAQTLAAVQQAARQLNGELSSRLQ
                     PFKDTLLEIIVPLESALEFVEDDLPEDITSHLVQSLETLITRLRAVVDTFQSGRLLKE
                     GLKITLTGCPNVGKSSLFNSLLMQERAIVTSIPGTTRDSLSELININGIPVLLTDTAG
                     LRSSADVIEQLGIERTEQAVVDADFVVVVIDGSQPLTDEDKDVLAKATKRNYLVALNK
                     SDLKSFNVSNLQDTNNHAPVISVSAKTRDGLDELINAIMEPFTAHGIPETDFLITNAR
                     HYDLLKRTISSLQSSQELLKRGSSEEITLTGLHNALRFLGEITGETTPEDILTQIFST
                     FCIGK"
     gene            30679..32547
                     /gene="mnmG"
                     /locus_tag="VF240_05560"
     CDS             30679..32547
                     /gene="mnmG"
                     /locus_tag="VF240_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX7174730.1"
                     /translation="MGYDEVFDVIVIGAGHAGCEAASAAARMGSQTALVTLNLDMIGQ
                     MSCNPAVGGIAKGHLVREIDALGGIMGHTIDRTGIQFRLLNRSRGPAVRAPRAQADRS
                     FYRTEMRRTLENTPNLHLIQGLVIDFIVDKDHVRGVELQDTRRLRADAVIVATGTFLN
                     GLIHTGRRTYTAGRAGEPASIELAESLKKLGFPVGRLKTGTPPRLDGRTIEWDVFEPQ
                     PADERPVPFSFSTESIEQPQVNCFIGYTNETVHKTISDNLSESPLYSGQIKGVGPRYC
                     PSIEDKIVKFPSKSRHQLFLEPEGYDTHEVYLNGFSTSLPSDLQHELVHQIRGLEEAR
                     IIRPGYAIEYDFVDPRELTPFLQTSRFKGLFFAGQINGTTGYEEAACQGLIAGINASL
                     FVQGREGFRLGREESYIGVLIDDLITHGVDEPYRMFTSRAEYRLQLRYDTADARLTSY
                     GYELGLMGDDGWQKFLRRRNRISQLRLSLESTKFNRTHPEYSYISTLLDKDLGDSFSL
                     SSFVHNPGIAPEVICQFLPANLQTGTSISELESALADVLYAGYISFQQNNIERLYHSD
                     SVPIPNNFKFGLVSGLSNEMVERLERTAPQTFGQARHIPGLTPGALTTLLYHLKLN"
     gene            32699..>32917
                     /locus_tag="VF240_05565"
     CDS             32699..>32917
                     /locus_tag="VF240_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7174731.1"
                     /translation="MAKIIAVANQKGGVGKTTTAVNLAAALATSELRVLLVDADPQAN
                     ATSGVGIQRGSLRRSLYHILVLDERVSNV"
BASE COUNT         6665 a        10504 c         9211 g         6537 t
ORIGIN      
        1 ccggtgcgtc ttacaccacc cccgcagcca ccactaccct tccggcaacg atcccgtcac
       61 agcagggcgg ctacggaacc gtctccgtgt cttacgcgct agacggcctc cagaacggcg
      121 cgccggacgg catcgccctc atcgggccgg gcaacgtcgt catccagttc ctctgctacg
      181 agggcacctt cgcggcgacg actgccccgg cctcggggca gacttgcacc gacatcggag
      241 tgacggaggg gacttcgacg cctgtcggtt tctcgcttca gctcactggc agcggcacga
      301 cctacggtga cttcgcgtgg gcggcagagc agcctaacac gcagaacaat gtcaacacgg
      361 gccagtcctt cggcgccccg acccccacgc ccacgcccac gccgactccg actcccgaac
      421 ccacaccgac cccgactccg acccctgaac ctactccgac gcccacgcca acgcccgtcg
      481 cgaccaacgt catcatcaac gagcttgact cggacacgcc gggatcggac acggccgagt
      541 tcatcgagct ttacgacggc ggcgtcggca acacttcgct gaacgggctg gtcgtcgtct
      601 tcctcaacgg gggcgtagcc aacgacccgt cttacgctgc cttcgacctc gacggctttt
      661 ctaccaacgc ccagggctac ttcacgctcg gcaaccccgg agtgagtggg gttgatttga
      721 ccttcaaccc cggcgcgagc ggtcttctac agaacggcgc ggacgccgtc gcgctctacg
      781 cggccagcgc gtccgacttc ccgaacggga cgccgttgac gacctccaac ctgattgacg
      841 ccgtcgtcta cgacacggac gacgcggacg acaccggcct gctggctctg cttaactcgg
      901 gccagccgca ggtcaacgag aacggcgaca gcgacgtcgc gaacgattcc atcgggcgct
      961 gccccaacgc caccggcggc gcgcgcaaca ccaacaccta cgcagcgggc actccgtcgc
     1021 ccgacggcgc gaacacctgc acgcccccgc cgcccccggc gaccaccaga acgatccccg
     1081 agattcaggg caacggcacg gcttcccccc acgccgggac ggcggtcatc acgacgggca
     1141 tcgttacggg ccgcaggagc ctcggcacga ataacaacgg cttctacatt caggacccac
     1201 tcggcgacgc taacacgaac acctccgacg ccgtcctggt cttcaccggg agcaccgtcc
     1261 cggcggtcgc cgtcggcgac tcggttcaag tgacggggac gatcacggag ttcgacgcat
     1321 cgacggacga gccggacggc gtcgcgcccc ccgaccccaa gaccaccacg gagatcaccg
     1381 cccccacgac cgtcgtcctt tccaccggca accccttgcc ggcggcgctc tcttccgcca
     1441 tcttcgaccc ggccgccatc agccgcggcg cggagttgga gaagtatgag cacatgcgcg
     1501 tgagcgtcgc tgcgctcacc gtctcgcagc cgacgaacaa cttcggcgag ttctggggcg
     1561 tggagccgac gcgcccgcgg ccgttccgcg agccgggcat cgaggccggc gaccccgtcc
     1621 cggccgccga cgatggcccc tacgcgggct tcccgccgcc cgccgtgccg atcttcgacg
     1681 gcaacttcga gcgcatcatg gttgacaccg gcgcggccct cctgcccggc ggctccaccc
     1741 gccgcccgca ggtgcaggtg accacgggca cgctcgtcac cgatctcgtc gggccgctcg
     1801 acttcgcctt caaccagtac cgcatcgtac tcgactacaa cgcggcgccc ggcgcctccg
     1861 gcggcctcac ggcggccgtc cccgtgcccg cgcgcaccaa ctcggagttc accgtcggcc
     1921 acaccaacct ggagaacttc accgcctcgg acacgatcaa gctggagaag gcgtcgctcg
     1981 ccatccgcaa catcctgcgc acgcccgaca tcctcggcct catcgagatc gccgacctca
     2041 gctcggcgca acagctggcg accaagatca acaccgacgc cgctgacccc ggcatcaatt
     2101 acgtcgcctt cctcggcgag accagcttca cgcaagacat cggctacctc gtcaacgccg
     2161 cgcgcgtgac ggtggtcggc acgccgcagc agtatcacgc gggcgacacc ttcacctact
     2221 gcggcgtgac cgacgctctc cacgaccgcc cgtcattcat cctggaggcc accgttcagc
     2281 aactcggcgg cgggacgctg cccgtgacgg tgatcctcaa ccacactaag tctctcatcg
     2341 ccgttgacag ccccagcccc ttcggcacct gcggcaccgg cacggagggc gcgcgcaacc
     2401 gcgagaagcg gcgcctccag gccgaggaca tcgccgacct catccagtcg cggcagaccg
     2461 agaacctcgt cttcttaggc gacctcaacg ccttcgatgt caacgacggc ttgacggacg
     2521 tgaccaacac gctccggggc gtgcccccgc cggccgagca ggtggtcgag ccgagcgtgc
     2581 ccgaccgctg gacttacgag ctgacgaatt tgctcaccac gctcacgccc gaccagcgtt
     2641 actcctacgt cttcgagggc aacgcgcaga cgattgacca cgtcctcgtc aaccagcaga
     2701 tgttggcgct caacaactcc ttcgcctacg cgcgcgtcaa cgccgacttc tccgaggctt
     2761 acgccatcga caacacgcgg cccgagcgcg tctccgacca cgacgcgccc gtcgcctact
     2821 tcacgctcct cgccgactcc gatgacgacg gcgtgaatga cgacgtggac aactgcccga
     2881 acgtctccaa cgccgatcag acggacacgg acaacgacgg gcagggcgat gcctgcgacg
     2941 cagacgacga cggtgacggc gtgaccgaca ccctcgacaa ctgcccgctc acggccaaca
     3001 cgaaccaagc cgacaacgac gccgacggct tcggagacgt ctgcgacgcc gacgacgata
     3061 acgacacagt cgtggatacg gccgacaact gcccgctcac tgcaaatacc gaccaagccg
     3121 acaacgactc tgacggtcag ggcgacgcct gcgaccctga cgacgacaac gacggggttg
     3181 ccgacacggg agacaactgc ccgctcgcgg ccaacgccga tcaggccaat aacgacggcg
     3241 acgcacaggg cgatgtctgt gacgccgacg acgacaacga cggtgttgcg gacggggccg
     3301 acaactgccc gttcaccgcc aacgcgggcc aggccgacaa cgacgttgac ggcatcggcg
     3361 atgcctgcga cgccgatgat gacaacgaca cggtcgcgga tacggctgac aactgccagt
     3421 tcacggccaa caccaatcag gcgaacaacg acggcgacgc gcagggtgac gtgtgcgacg
     3481 cggacgacga caacgacggc gtgccggaca ccgcggataa ctgtgacttc acagccaacc
     3541 cggatcaggc ggacgcggac ggcgatggga tcggcaacgc ctgcgagtcg gacgacgata
     3601 acgacggcgt gcccgacacg gcggacaact gcccgctcac gcccaacccc aaccaggccg
     3661 acttcgacgg cgacggcatc ggcgacacct gcgacccgca gaccggcccg ccggtgaaca
     3721 agaatcagtg caagaacggc ggctggatgc ggttcgacgt gccgcgcacg ttcaaggatc
     3781 agagcgactg catccagtac gtcaacaccg gcaagtgatt cccggcgcgc gcctctggtg
     3841 gtgaagattt agaggacgcg ctcggttccc gaagcggaag cgccgcccga cgccgaaggg
     3901 ctgcgggcgg cgcttccttc tcgctcttgc gtccgacccc gaattgttgt atttaacatc
     3961 gcgtttactc taacgtaagg gtcgcgcaag ccactccccg tatggtataa gtttgatttc
     4021 aaacctggcc gtcattcact tttaagcagg acaaccagac gaaccgggac acgagcgaaa
     4081 gtctttctgt gtcccgcctc agacaactgt agtgcatcat ccgcgataca tcgcccaggc
     4141 tctcgcgcgg caggcaatgg cgcccgcccg ccgtgcggcg cgctccgcca gtccccggac
     4201 gctgcgccga aacaccccaa cgcaccaatt tgatagaagg agtttcttcg atgtttaagt
     4261 tttttcgcct tcccctcgcc gcgctcttcg tgatcgccac gacctgcggc gcggccttcg
     4321 cccagtccac cacggacggc gccatcggcg gagtggtccg cgacccgaac ggcgcggtcg
     4381 tggtgaacgc ccccgtcacc gtccgcaatg aggagacgaa caaggagacc gcggccacca
     4441 ccgacgacga gggccgcttc cgcgtcgtcc aactccagcc gggcagctac accgtcacgg
     4501 tcaacgccac cggcttcggc cccttcacgg cccagaaggt cgtcgtcgag gtcggccgcg
     4561 tcaccccgct ggaggcgacg ctctccgtga ccggcaccag tgagacggtc gaggtgacga
     4621 gcgaagcgcc cgtcatcaac accgagcggc aggacttgtc caccaatatt aaccagactt
     4681 cgatcaacaa cctgccgatc aacggccgcc gctggtcgaa cttcgcgctc ctgacccccg
     4741 gcgcggtgcc cgatggctcg ttcggtctgg tctcgttccg cggcgtctcc ggcctcctca
     4801 acaacaacac ggtggacggc ggcgacaaca atcaggcgtt cttctccgag gagcgcggcc
     4861 gcacccgtct cagctactcg atcagtcagg actcggtgcg cgagtttcag gtcaacacct
     4921 cgaactactc ggccgagtac ggccgcgccg ccggcggcgt cgtcaacgcc gtcaccaaga
     4981 gcggcaccaa cgagtttcac ggcaacgcct tcttctacgt ccgcgacgac aaattcaacg
     5041 cgcgcaaccc gcagctcgcc accaagaccg aggacaaccg caagcagttc ggcggcacca
     5101 tcggcggccc catcgtcaag gaccgcgcct tcttcttctt ctcttacgac cagcagaagc
     5161 gcatcttccc cggcatctcc acctacagca ccaacacctt ctcgacggcc aacttcaaca
     5221 acgcgaacct gctcctccgc gggctgacgc aggcgcagat cgatgcgacg cgctccttcc
     5281 tcgacagcct caccggcccg ttcgaccgca agggcgatca gacgatcttc ctgccgaagc
     5341 tcgacctgcg tatcaacgac agtaacgccc tcgccgtcac ctacaatcgc ctgcgctgga
     5401 aatcgcccaa cggcgtgcag accacttcca gcctgacgcg cgccaccacc agcatcggcg
     5461 acgatctggt ggacgttgac tggatcaccc tgcgcctgac ctcgaccatc tcgtcgaacc
     5521 tgatcaacga ggcgcggttc cagtacgggc gcgacttcga gcgccagacg taccccggcc
     5581 cgcttccggg cgagccgacg acggcccccc ccggcggcgt gccgccccag atcgggctgc
     5641 ccggctacac catcggcaag ccgaacttcc tcgaccggcg ctccttcccc gacgagaagc
     5701 gctggcagta cgccgacacc gtcacggccc tcgtcggcaa ccacaccttc aagttcggcg
     5761 ttgacatcaa ccacgtcaac gacatcaacg acaacctgtt caccgagtca ggctcctacg
     5821 tctactcgac gaccagcgac ttcttcgttg actacgccaa cttcctctcg aacaacgcgc
     5881 tccgcgccct gagcgcgacg accgcgacca ccaacccgct cggccgctgc tccggctcga
     5941 cgcgccgcgc cggacagtgc tacaacggca ccttcaatca gggattcggc acgcccaagt
     6001 tccagttcga gaccgacgac tacaacttct tcattcagga cgactggcgc atcaccccgc
     6061 gcctgagcat caacctcggc ctgcgctacg agtatcagaa gctccccgcg ccgcagattc
     6121 cgaacgcgac cttcgactcc gacccgcgct tcaccgcgaa gaccggcgtg ttcccggccg
     6181 acaagaacaa cttcgggccg cgcttcggct tcgcctacaa catcacgggc gattcccgca
     6241 acgtcgtccg cggcggctac ggcatctact acggccgcat ccagaactcg gtggtctcaa
     6301 acgccatcac caacacgggt aacccggcgg ggcagacgca gctcaccctg accacggcca
     6361 tcttcccgca ggcgttccag acggcggccg acgccagcgg cggcacggcg ccgattccga
     6421 acatcgtcgc cttcgcgtcg aacatggcca acccgcagat tcatcaggcc gacctgatct
     6481 tcgagcacga gctgacgcgc aacaccgtcg tctcggcgtc ctacatcatg agcgccggcc
     6541 gccgcctgcc gaccttcgtc gatgtcaacc tcgccttccc gacctcgacc acggcgattc
     6601 ctttctccgg cgggccgttc gacgggcaga cgctcagggt gccgcgtttc accacccgca
     6661 tcaacaacgc cttcaacgcc atcaccgaga ttcgcagcac gatcaagtcg gagtacaacg
     6721 cgctggtgtt gcagttcaac cgccgctaca cgaacgggct gcaattccag accaactaca
     6781 ccttctcgaa ggccaaagac aacgggcagg cgacgggcac gttcacgaac acgaacgccc
     6841 cgctcagccc ctacgactac agtctggagg tggggccgtc gaacttcgac atccgccacc
     6901 gcttcgtggc gagcgccgtc tggactcccg acttcttcgg cgaggaccag aaggtcgggc
     6961 gggccatctt caacggcttc aacatcgccc cggtcttcgt cctccagtcg ggcttcccct
     7021 actccatggg catctcgggc acgcccaccg ctgcggcctt cggcccgacc atcgtccgcg
     7081 gcggcagtaa tctcaccggg acgacggcca cgggccgcgt ccccttcctc gggcgcaaca
     7141 gcttacgctt cccccgcttc gccaacttcg acctgagggt ctcgcgccgc ttccgcatca
     7201 cggagacaat gaactttgaa gtgctggccg aggcgttcaa catcttcaat cgcacacagg
     7261 tcatcggcaa cctcgacacg accgcctaca ccatcaacaa caccaccaac aacctcatct
     7321 tccagccgac cttccagagc atccagccgc ccgccggtga gacgctctac aaggcgcggc
     7381 agatgcagtt cgccctccgc ttcgagtttt agcacaagcg ttcggccgag acgaccacaa
     7441 cagagggcgc gacactccca cgggggcgtc gcgccctctt tgtgtccgca cacgcggcgc
     7501 ggctgtacga aaatggacac accacgcggc cgcgcctctc acagcacggg aagcgcacgc
     7561 ggaggcataa cgccgtttga aagaatgact tccgccccac cggcgccgag acgagccgcg
     7621 cggcacgtcc gttgccatcc tcacaggctg aggaacactg tagatttctc ttgctcatca
     7681 atcgttgaag ggatagctaa tcacacgagg aggtagaact catgggtttt ccgaataagg
     7741 atgaagtcga aggcaaattc gaccaggcga agggcgccat caaggagaac gtcggtgacg
     7801 ccatcgacga cgagaagatg cagtcggagg gcgctgccga gcgcgccggc ggacatgctc
     7861 aggagggctt cggcacggcc cgccgcaagg tcggcgagac catcgaagac atcggcaaca
     7921 aggtcggcgg gtagcatccg ctctcgtcag tagacacttt tcacggcgcg gaggccgcgg
     7981 ttttgcccga gtatggacag gcccgcggcc tctcgctggt tttccctcga caaactgttc
     8041 caacgcgcct cggcctctcg caactccggc ctgaggagcg ccccgcgcgg tcacgtttga
     8101 ccgagcgagc cggctttgtc ataaaaccca cgctcgcacg gctccccctg caagcccccg
     8161 ctggcagttc ccctttgggc agacatcttt cacgccgcct tcgagacgcg ccactcccca
     8221 acggaaagcg ttcggcgcgg ggaaacccag ccatgaaaat cgaatcccga cgcctcgccc
     8281 tgccgctgct gcttgtcgcc atcccgctcg tcgccttcgt cgccttcgtc acgtggcgcg
     8341 tcgtcacacg cgaagcgccg cccgacgcgc aggagccggt ggacgagatc atccgcagca
     8401 cggacgtgcc gatggtcgag ccggtcgcgc gcgacctcgc ggccatcaag gaggcgggca
     8461 cgctcaccgt gctcgcgccc tacaactcga ctacctactt cctctaccgc ggcgagccga
     8521 tgggctacga gtacgagctt ttgcgcgcct tcgcgcagga gcacggcctc gcgctcaaga
     8581 tggtcgtcgt caccgaccgc aagagcctct actcgctcct caactcgggc gagggcgaca
     8641 tcgccgccgc ccgaatcgtc cccaccgaga cggatcgcgc ccacgtcgcc ttcacgcgcg
     8701 agctttaccg gacggagccg gcgctcgtcc agcaggagca gccgcccgcg gaggccaacc
     8761 tctcacacgc cgccgaacgt gcgctccaac ccgcgcccga cgcgccgctc ccggaagatt
     8821 tggagattca ggcccgcctc atcacgcgcc ccgcgcagct cgccggcgaa accgtcacgc
     8881 tgcccgagaa gtcaacttac aagcgcacgc tcgtcgagct ggccgacgag atttcgggcg
     8941 agatacgcgt cgtcgagatg ggcggtaaga tggaggacga ggcgctggcg cgtcaggtgg
     9001 cgagcggcga ggtggagttc acggtgatgc agcgcaacgt cgccgaactc aaggaggcgg
     9061 agttcaccaa cctcaaggtg cggcccgtcg tcgggcgcgt gcacagcgtc tcgtgggccg
     9121 tgcgcccgaa ctcgcccgcg ctgctcgaag agttgaaccg ctggatcgcc gagaagaaaa
     9181 gccaggcgct tctggccacg ctctacaaga aatatttcat cgacgtgaag aagcaccgcg
     9241 agcgcgcggc gagcgagtac ctgacctcct ccaccggcaa gctctgcgag ttcgacacgc
     9301 tcttgaagcg ttacgccggc gagctgagtt gggactggcg gctgctcgcg tcacaggcct
     9361 ttcaggagtc gcgcttcaag ccggacgcgc gctcctgggc cggcgccacg ggcctgctgc
     9421 aactgatgcc gcgcacggcg cgcgagttcg gcgtgcagga catgaacaac cccgagcaga
     9481 acgtgcaggg cgccgtccgt ttcctgaggt ggctcactga ctactggacc aagcgcatcc
     9541 ccgacgagca ggagcgcctc aagttcatct tggcctctta caacactggc gccgggcacg
     9601 tcgaggacgc gcaacggctg acggagaagt atggcgggga taagttgaag tgggacgacg
     9661 tttcctactg gctcttgcag aagtcaacgc agcagtacgc cgccgacccc gtcgtgaagt
     9721 tcggctactg ccgcgggctt gagccggtca cttacgtcga gcgcatcctt gagcggtttg
     9781 aacactaccg gcagttcgtc gtcgatgcct aagttggcat aaacagtacc gcgagcggta
     9841 gcgagcgggt gtggttacgc aatacgcaac ccgctcgcta cccctcgcgg tactgttttt
     9901 tattcgcgac cgccgcggcg gcgtccgtcc ggcggctcat cgagcaggcg gtcaacgccg
     9961 cgacgcccga acatctcgga gacccgtggg tcgtacctgc gctcgtaatc gtcgcgctcg
    10021 cgctccctct cgcgcggcgt cagcgcataa cgcggcctcg aacccttatc cccgccttca
    10081 cgctcaggcg cacgccaaat tctctcttca tcacgccgcg gcgcgtcttc gtagtcgcgc
    10141 cttcgcgtca tcctctcgtc gcgtcgcggc ggaggcgcgt cgtcgcgcac acgcccttct
    10201 tcgcgcgtcc tcagcggacg ccgctcttcc cgtgcgcctc ggcgtggctc ctcgcgcctc
    10261 gacatgacag gggcgaaaga gctgcgacgg ccctcgccgg cgtaaccctg acggccacgg
    10321 ccttcgcgct cggcgaggcg gcggaagcgt tcgaggtctt cttcgagctg ccggcccggt
    10381 tgcacgccga agaagcgcgc ggcctgcgcg cccgagcgcc cgagcggcgt gccgaagccg
    10441 aggacgacgt gcagcagcgt cgtcccctcc ggcgtctcgc tgatgacggc ctcgccgtcc
    10501 gcgtggatgt cgccgcgcac cgaacgccag acgaggcgat ggtctggctc atagacggtc
    10561 aactccgcct cccactccgc gtacacgtcc ggcacggccg tctcgctcac ccaacgcgtc
    10621 acgcgccgac ccaagcgccg cacctcgcgg atgttccgca tccaccgcgg gaaactctca
    10681 aagtcgctca aacggtcgaa gaccacctcg accggcgcgt cgatctcgac gaacctctcg
    10741 gccctctcca taaatcctcc agagaagagg tcagaggtta gaggtcagag gtcagtgaag
    10801 agcgtcttgt ctttcactga cctctaacct ctgacctctg acctctgttt tattcctgcc
    10861 ggatgtactc ggcgcccagg tcttcgagca gcttctccag cgcgcccgcg tcctcgtctg
    10921 acttcgactc gacaccgagg aggatgccgc cggcgcgcaa agacgcgtcg tagaccttcg
    10981 cacggtgctc ggggatgccg gcgccgacga gcgcgccgat gagaccgccg gtcgcgccgc
    11041 ccgcgcccat cccggcgagc gccgccgcga gcgggccggc aacgacgatg ccgatgccgg
    11101 gaatcaccat cgtcgtgccg accgcggcga gcgccgcgag caccgcgccc accgcgccgc
    11161 ccactgcgcc gccgatgccc gcgccatcgg ccgcgtgcgt ccgcgtctcg atggcgaact
    11221 ctttagtctt cgtcgcgtcg gacatcaaca cgctgatgtc ttcgcgcgtg tacccccgct
    11281 tgatgatggc gtccacggcg gcctccgcgg ccacgcgcgt cttaaaaact cccgtcacca
    11341 gcttggtcat ttcgttcgct ccttcggctt cttcagcccg cctgagttga aaaaaaacag
    11401 agtcgtccga tccttcagga tggcaacact tgtgccggcc gcgcccgcgc gcgggcttgc
    11461 gcgaacaggt ctattcgaca ccgacagata cggccccggc ggcaatattc cgcgcccggc
    11521 gcggcgacgt accaaaccaa aacaaaacgg gcgcgtgtgt tctgttcagc atcagccact
    11581 tgctgaccct ccgcgtgaac tctgcgccct ctgcgcctcg gcggtgaatg cgacgctccc
    11641 caagctcaac tcacagacga aagcactaac ccccgccatt agtaactgcc gcgtccgctg
    11701 gtgtaactta ggccgccgca agccgaaacg aatgggggag agcagcattg atcaaagccg
    11761 tcatcttcga catcgacggg acgctcgtcg attccgttga cctgcacgca aaggcgtggc
    11821 gggaagcgtt ccggcacttc gggcgcgagt tccctttcga gcgcgtccgc taccagatcg
    11881 gcaaaggcgg cgaccaactg atgcccgtct ttctctcgcc cgatgaactc gaacgcttcg
    11941 gcgacgagtt ggagaagcac cgcggcgagc tgttcaagaa agagtacatg ccccgcgtgc
    12001 gccccttccc ccgcgtgcgc gagttgttcg agcgcatccg cgccgacggc cgacgcatcg
    12061 ccctcgcttc ctcggcgaaa gaagacgagg taaaggtcta caggaagatc gccgacatcg
    12121 gagacttgat tgaagaacaa acttcggccg acgacgccga gcgttcaaaa ccgtacccgg
    12181 acatcttcga ggccgcgctc gaacgcctcg acgacgtggg gccgaaagaa tcactcgtcg
    12241 tcggcgacac gccttacgac gcacaagccg ccggcaagat cggcctccgc accatcggcc
    12301 tcctctgcgg cggcttcccc gaggaagacc tgcgcgccgc cggctgcgtc gaaatctaca
    12361 aagacccggc cgacctcctc gcgcgctacg aagaatcgtt aatagccgtc aatcgtcaat
    12421 cgtgaatcgt caatcgaatg ggttgaattt catccgactt ccgattgggc gttcaggccg
    12481 agtcagattc aacctctatt cacgattgac gatttaccat ttacgattga cggcctttaa
    12541 gccgtgagaa ttcttcagat cagttcagcg cgcacgcttg gcggcggcga gagacacttc
    12601 gccgatctgg cgaacggact cgcccgccgc gggcacgagg tctacgccgc gctcgcgccc
    12661 ggctcgccgc tgcgcgacga actccgcgac ctccccgccg ataacgtcct gacgctttcg
    12721 ctgcgcaacg ccctcgacct caagagcgcg tcgggcctcg cacgcctcgc gcgcgagcgg
    12781 cgcgtcgaac tcattcacgc acatgtcgcc cgcgactaca cgctggccgc tttcgccgcc
    12841 cggcgcgcgc gcggcgcgcg gttcgtcgtc acgcgccacg tcctgttccc gctcggccac
    12901 gcgcaccgca tagtgctcgc ccgcgccgcg cgtgtcatcg ccgtctcgga ggcggtcgca
    12961 cgcacgctcc gcgcgcgcgg cgtcttcccc gaacacaaaa tccgcgtcgt ccagaatggc
    13021 atcgacatcg agcgactgga gcgggccgcg tcaaagctcg attgcgggac ttaccgccgc
    13081 gaactcggcg tgcgcgcgcc gcaaatggtc ggctccgtcg gcgagctgag cgaggtgaaa
    13141 ggacaggccg acctcgtccg cgccgccgtc gccgtcctgc gtgagatggg caacgcggtc
    13201 gagttcgtca tcgtcggcga ggacgcttcg cgcggcggcg gcaaccgcgc gaaactcgaa
    13261 gcgtcgatct ctgaactcgg cgtcggagga cacgtgcggc tggcggggcg gcggggcgac
    13321 gtggcggagt tcatgtcgtg cctcgacgtg ttcgtcaaca cttcgcgctc ggagtcgttc
    13381 ggcctggcga cggtcgaggc gatggcgtgc ggcgcggcgg tcgtcgccac agccaccgac
    13441 ggctcgcgtg agttaatcga agacggagta acgggtctgc tcactccggt cggcgacgcc
    13501 ggagcgctcg ccgctgccgt cctccgcttg ctgcgggacg aaagtctgcg cgcgtctctc
    13561 ggcgacaggg cgcgacggct ggcgcgggag cgttggagcg tcgaacggat gatcgacgag
    13621 acggagcgcg tgtacctcga agccttatca caggacgctt gaggaagggc ggggcaagtc
    13681 gcggctaaaa aaaatcgggg gcgaagccag cgcggctcgc ccccatattt tttgtcagcc
    13741 ccgtgctagg cggcgttcct gtactgctgt cgcggatcgt actcttgccg gaggtagcgc
    13801 cgcgcaatga atttgagaac cgccgagacg tgcttgcact cgtatgtcgg ctcggcgtcg
    13861 ccgcgaaaga tgaagtcggc gcaggtgcag acgagtctct tcgactcggg gttgcggaag
    13921 aggtgatagt tctgctccgc gtgcgactcc gaatgagcca tccacccgta aggctcggcg
    13981 tccgtgacga cgaccctctc cgagcgggcg gcgcgctgct ccgcgggcgt aaaagtttgt
    14041 gatttgagcg tctgtttttt tgtcttcata aaagtctcgt tccttccctg attcgaccgc
    14101 cgcgcgcccg ccgggcacgc tgctcaactg tctgattcat taaggtttag atgctcggcg
    14161 agcgccggga gttgattcgc tcgcgcgtca atattttacc agatgccaga ccttttcgcg
    14221 ccgtcttgac gctcgaaaaa tacgattcgc tgtggcgacg ctaacgcgcg tcacgcttag
    14281 attatttgtc tgattgcgtt tatcatttga atgaaacttc agcgggacag aacggacgca
    14341 agcgaggtga gcgacgatga gcgcgacgac gcgtgcggtg cgcggactgg agatgacata
    14401 caacgtgcgg ggcggcggcg ctcccgtcgt ccttctccac ggcttcccct tcaaccacac
    14461 gatgtggcgc ggacaggtcg aggcgttaag cgagggccac cgcgtcatca cgcctgacct
    14521 gcgcggtcac ggcggaacag gtctggtcga aggcactacg tcgtcgtcga tggaggagat
    14581 ggcgacggac gtggcggcgc tgctcgatga gttgaacgtc tcagagcgcg tcgtcctcgg
    14641 cggcctctcg atgggcggct acgtgacgct cgccttcttc agactcttcg ccgaccgcgt
    14701 gcgcgcgctc gtcctcgccg atacgcgccc gcaacctgac accgaagagg cacgtcgcgc
    14761 ccgcgaggag accgcgcagc gcgcactgcg cgagggaatg cagacggtcg ccgacgcgat
    14821 gctgcctaaa cttctcgcgc cctcaacaca cgaagagaag ccggaggtga tcgagcgcgt
    14881 gcgggagatg attctggcga cgaggcccga gggggccgcc gccgcgctcc gcggcatggc
    14941 cgcgcgcagg gatcagactg acctcctgca agagatcgtc gtgccgaccc tcatcatcgt
    15001 cggaagcgag gactcgctca ccccgccgaa ggattcggag gccatgcacc atgccatccg
    15061 cggctcgcgt ctggtgatca tcgaaggcgc cgggcacgtc tccaacctcg aacgtcccac
    15121 cgagttcaac cgcgcgctcg aagatttttt gaacgggatc gagtcttaag ccgacgaaca
    15181 aagcacatga acgaaacgtg gaaatatcgc attgaggagc gcgtccgttg gggcgacgtg
    15241 gacgccgcgc gtatcatctt ctacggcgca tacattcgct tcttcgagtt cgccgagacg
    15301 gagttgttcc gcgccgtcgg tctcccctac ggcgtgatgt tcgacgagct ggacgtgtgg
    15361 ctcccgcgtg ctcatcttga atgcgacttc agacgcgccg cgcagctcga cgacctgctc
    15421 gaagtcggcg tctacgtcgg gcgcatcggg aacaagtcgc tgcgcctgaa cttcgaggtg
    15481 cggcgcaagg gcgagggcga gatgctcgcc gaggcccatt tcgtcctcgt cgccgtgcgc
    15541 cgcgacacgt tcgagaccgt caccgtcccc gacgagctac gcacccgcct cgctccctat
    15601 acgcagccgc taaagcgcgc gttatcttag acggcctcga caaccccgcc ctgcattgag
    15661 cggcgcgact cacaccggcc ggaatgcgcg ccgcaccagt ttccttttga gattcacgta
    15721 gccctgctcc ccggcgatga gttcacacag tgtgcgtcgg acgccgcggt gcgcccgcgc
    15781 gaactccacc atgcggtcgg tgaaggtcgc ccccatgaac gttccgtaga agcgcccgcg
    15841 catctgcgac gcccgccgca gctcgcgtcc aaaatccccg cgccacaatt cgtcgtaagc
    15901 ttcagcccgg ccctcaagcc acgcctccgc aaacaactcc gccgagcgca acgcgtagta
    15961 gatgccctcg cccgtcaccg ggtcagcgaa gccggccgcg tcgcccagaa gcgcccagtc
    16021 cttcccggaa gcgcgtcgcc tctccagagt ctccggtgtg agaccgggga tgcgcgccgc
    16081 ataacgctcg gccgtccgac gcagctccgc ctcgatgctg cggtcgcgct cgccgccccg
    16141 tgcgacgcct tcgccggcag tccagacgcg cgcggtctcg tcgtcgcgcg tgcggtagta
    16201 gccgaccata aagtcccaga ggagacggtc aagcgcgcgg tggtcgaagg acacctgcgt
    16261 ggtggcgatg ccgaaagaga tgtgatcgag acgcgggaag gcccacgcgt agccggccca
    16321 gccgggcagg aaggcgatga cggtcggcgc gtccgcgccc tcgtcaagcg gcgtgcgata
    16381 gccgaaggcg acctccatct cggcattggg cagtgggccg gcaaggcgtc gggcgatggg
    16441 actgtttgcg ccgtcggctg cgactatgac gcggccgctc cacgcgcgcc cgtcgcgcgc
    16501 gcgcgctacc cagccgcctt ccgtctcctc acacggcgtg gcggagacgc gcacgggcag
    16561 cacttccgcc ccggcaatac gtgcgcgttc gagcaggtgc gagtcgaaga cgcggcggga
    16621 gtagatggcg aaggggtctt gaagtttcag gcgcacccgc ctgcccgctg gcgaatgcag
    16681 atccacttca tcgaccgtac gtgccgctgc ttcgagtaag aagggccacg ccttaagcgc
    16741 cttcgaggtg acgccgccgc cacacgcctt cgcctccgtg tgcgggcgcc cgtcgaagag
    16801 cgccacccgc gccccgcccc gcgcgagccg ctcggcggcg aaggaacccg ccggccccgc
    16861 cccgacaatc agcatgtcgt acttcgtatg attagccatc cgactccatt ctaaacatcc
    16921 cgactccgcg ccgggcgcga cccttatctt acacgatagc gcctttgcgt ctctctccgc
    16981 ttccctctgt tagcacgacc gacggtatgg tagaattttg gcatctcctt atagaacgtg
    17041 ttcagggtta gccgaagttt taatcagagc gtccccgctt cccctttaca gccctccgcg
    17101 ccgtcgcgtc tgcactgtca actcgggcac ctgtcatatg gcaaccggct cgatggtttt
    17161 cgtaggcatc gccccgcacc cgccgatcat gatcccggag gtcggcggcg agattgtctc
    17221 cgaggtacgc ggctccgtcg aagcgatgga agaactgacg cgccgcattt cggagagtgg
    17281 cgccgagagc gtcgtcatca tctcgccgca cgcgccgctc gaagcacgcg ccttcgtcgc
    17341 ctatggcggg ccgactttgc gtggcgactt cgcacagttc cgcgccccac aagccatcat
    17401 cgacatgcca ctcgacggcg atctcctcac cgccatcgcg cgcgaggccg ccgatgaggg
    17461 gcttcacgtc atgccccttg aaggttacga actggatcac ggcacggcgg tgccactcta
    17521 tttcctgcgg cgtttcggct gggacggccg cgtcgtggca ctcggctaca gcttcctcac
    17581 ggacgaggac cacatcagat tcggcgcctg tgtccgtcgc gccgtggacg ccgagggccg
    17641 cgccgtcgcc tttgtcgcca gcggcgattt gagccacagg ctcaaacccg gcgctcccgc
    17701 aggctacaac ccggacgccc gacttttcga cgagcaagtc gtcgcggcga taagttcttc
    17761 agacccactt ggaatcgcgc gcgtggatca gggcctgcgc cggttggcgg gcgagtgcgg
    17821 ctatcgctcg atgcttgtgg ccctcggcac gcttgcgggg gcgcccatca attgtgaggt
    17881 cttacactac gaggctccct tcggagtcgg ctacctcgtc gcacaactgt tgcgcggcgg
    17941 cgacatgggg gaaaaatgag cggcgacgat aaacgcgtag acgagacgta tgtcccgccg
    18001 ccgtcgtcgg gcgatgcgga actcacttcc cccgaactga gtgatgagac caaaatacct
    18061 gcgctcgcac gcctcgcggt ggagactttt attcgtgaac gacgcctgct cgatagcgac
    18121 tgtgcgcccg aactgcccgt cctccgtcaa ccggcggcct gcttcgtttc aattaaaacc
    18181 ggccgcaacg acttgcgcgg ttgcatcggc acgattgagc cttcccgtcg aacgctcgcc
    18241 gaggaactta ttattaacgc cgtccatgcc gccacgcgcg acccgcgctt catgcccgtc
    18301 gatgagcatg aacttccttc cttacgctac tccgtagacg tgctctcgga acccgagccg
    18361 accgagtttg aatctttaga cccttcgctc tacggcgtga ttgtcgaaga ccagacgggg
    18421 acgcgccgcg ggttgttgct cccagacatc gagggcgtcg agacatcgac ccagcaggtt
    18481 caaattgccg cccgcaaggc tggtatcgac cccgacgagc cgttaaggct tttccgtttt
    18541 caggtaactc gctttcgcga atctggaaag cacacttctt caggttaagg atgacaggca
    18601 gggttcttta cttgtcccag aggttttata taaaaaaacc gctgcatacg cgagagtatg
    18661 cagcgggcaa tatccacagg aggaaaggaa ttacatctta tacgagcggg tgcggtgtgt
    18721 caaatagatt catcggacat ccgccgcagg caatttctta aacgtctaag ttcttcacgt
    18781 cgagggcgtt atcctcaatg aaccggcgtc gaggctcgac ggcgtcgccc atcaaaaccg
    18841 tgaagatttg gtctgtctca accgcgtcgt cgattttgac ctggaggagc gtgcgtttct
    18901 cggggttcat cgtggtctcc cagagttgct ccgggttcat ctccccgaga cccttgtaac
    18961 gctgaattgt caggtctttt ttggcggtgg ccagtacgcg ctcgagaagg acctcacgcg
    19021 attctatctg ttcactggag ccgttttcac ccagaacgaa cggggccgcc agccgatcct
    19081 ccaaagtctt gtagagatcg acggcctttt gaaactccac gtgactggca aggtcccagt
    19141 cgatgaggac cttaaaacct ccggcactgg tcgtctctat cgcccagaga ccgtgctcct
    19201 cgtccatagt caggtcggta cggtagccgg cctcggccag ggcgccctcc acggtggcca
    19261 tctgctcccc ttcgccatcc tggaaaacac tgcgaagcgt ggggttacct tcgtttcgta
    19321 gcaccccctg gcgaccgccg agagcttcca aaagcgcgtg gaggaggcgc gcgtcatttc
    19381 ccagccgtct ggcggcccgc tcacaatatc tgttcaactc aaccagttgc tccagagacc
    19441 gcgccagctc gcgaccctct gtgaccgttt tagctgacga ggaggtgact ttcaagtcgc
    19501 tcgtcgcctg ccgcatgagg tagcgcacca tagatctttc atctttgatg tactcttcac
    19561 gcttaccgcg ttttactttg aaaagcggcg gctgggcgat gtaaatgtgt ccggactcca
    19621 gcaactccgg catttgacga tagaagaacg tcagaagcag cgtgcggatg tgggagccgt
    19681 ccacgtcggc gtccgtcatg aagacgacct tgtgataacg aagcttgccg atgtcgaaat
    19741 catccttgcc gatgccggtg cccagcgccg tgatgagcgt tttgatctcg ctgtggctga
    19801 gcatcttgtc aaacctggcc ttctcgacgt tcaagatttt accttttaac gggagaacgg
    19861 cctgattccg acgatcccga ccctgcttgg cgctgcctcc tgccgaatcg ccctcgacga
    19921 tgtaaagctc gcagagggcg gggtcgcgct cctgacagtc tgcgagcttg ccgggaagca
    19981 tgctcgagcc gatggcgccc tttcgcacaa tttcgcgcgc cttgcgtgcc gcctcgcgcg
    20041 cgcgcgccgc gtcgatggac ttgccgacga tctttttagc caccgccggg ttctgttcaa
    20101 aatactcggt cagtttttcg ttcaggaacg actccacagg ccctttaacg tcggagttga
    20161 gcttaccctt cgtctgccct tcaaactgcg gctgcggcag ctttacggag ataacagcca
    20221 ccaaaccctc acgcacgtcg tcacctgaga gcgaactctt gaaattcttg gcgagtccgg
    20281 aggactgggc gtaggcgtta atcgtgcgcg tgatggcgga gcggaagccg gagaggtgcg
    20341 acccgccgtc caccgtgttg atgttattgg cgaaggagta gaccttctcg tcataggcgt
    20401 cgttgtactg gatggccact tctaccgaga gtgcgtcgcc aatgcgctcg aaatagagcg
    20461 gcttgtcgtg gaggatgctc ttattcttgt tcaaatgctt gacgaactcc gcgatgccgc
    20521 cccggtagta gaactcgtgt gagcgctccg gctcctcgcg ctcgtccctg atcgtgatgc
    20581 gtatgccctt gttgagaaat gccttctcgc gcaggcgctc ggagagcttg tcgaagctga
    20641 attccgagac atcgaatatt tcggagtcgg gcttgaacgt gattttggtg ccgcgcttgc
    20701 gcgtcttccc ggtcaccttg agagcggcga cgggtatccc gcgcttgtac tcctgctcgt
    20761 gggtctggcc gtctctccaa atctcaagac taagcaactc tgacagaaag ttcacgcagg
    20821 agacacccac gccgtgcagt ccgccggaca ctttgtaagc gttggagtcg aactttccgc
    20881 ccgcgtgaag tttggtcatc acgacttcgg cggccgacct cctctcctgc ttgtggtagt
    20941 ccacggggat gccgcgcccg ttgtcgatga ccgtgatcga attgtccaga tgaatcgtga
    21001 cctcgacggt gtcgcaatag ccggcgagcg cttcgtcgat ggagttatcc accacctcgt
    21061 agacgaggtg atgcagcccc agttcaccgg tcgagccgat gtacattgcc gggcgcttac
    21121 gcacggcctc gcggccttcc agaaccgtga tcgaagtcga atcgtaattg gtgcttttct
    21181 tttttggtat ggttactgtt gacaaaccca tctccttttt aaacgcaact cacgtatttc
    21241 taactcgatg ttttcgtatg ccagtgaggc ttgataccag cgtgttttag atgccattca
    21301 cgtcagtcgc gattgacgaa atcaattccg cctctgacat cctttcaccc gccgcgcccg
    21361 cggaaacctc gtatacgttt gagcgtgcgg cgaacctttt aacgaagtca tccttggagg
    21421 tcgtgacgaa cgtttgcgtg cgcccttcca gagattccaa cagccggtca agacgcatcc
    21481 gatcaagctc cgcatccacg tcatccatga tgaaaatcgg atactcgctg tgccatgaat
    21541 gatacactga aatggccgct aaatcaagta aaatcaacgc actacgctgc tggccggagc
    21601 tgccgaaagc gcgcatgtcc cgcccgtcaa acagtatttg cagatcgtcg cggtgcgtgc
    21661 cgatgagcgc gtatccagac gcgatttcgg ccggcaatct caacttcaag cgctcggcga
    21721 tgagcgcctc ataatcgctt aaatctccct taccttcgag cgaagacaga tacctgatgg
    21781 aaacctcttc ccttccgaag aaacggctct ccagcacccc gttcaggcgc tcgacgtatt
    21841 cgttgcgcga gcgatgaatg atagcgctca gtttcacgag ttgttcgttc caaggctcga
    21901 tcaactttcg cgcctcgtcg aaagccaact ccgcgtcgga ggcatcctga agcaggcgat
    21961 tcttctgctt gatcactcgc tgatagtccg acagtgtctg gacgtaagcg tgatgcagcg
    22021 agattacgcc acggtcgagg aatctgcgcc gcgcctccgg gccgccgcgc acgacctcca
    22081 tctcttccgc cgtgaaagcg accgtgtgga gctggctgag gtagctggcg accgcttcac
    22141 gtttggcgtt gacggatagt gttttggagt tgccctggag cgtaacctgt aggtctcgct
    22201 gcacgtcttg cgtgcgggtc acgcggccgc ggacgatggc gaggtcttcc ccgaagttga
    22261 tcgcgtcctg gagtctttgc gtgcggaagg atttcgacgt ggagagaaga tagattgctt
    22321 cgagccagtt cgtcttgccc tgcccgttct cgccgtagat gatgttcagc cccggccccc
    22381 agttaacttt gccgctcaag tttctgaaat tgtgtacctc aagaaattcg agcagcatgt
    22441 gtcgaatttt aacacatgct gctcgaatga gagagtgggg ccgatacaga gcctacgcgt
    22501 caatttcagg caatcagagg cgcatcggca tcacgacgta cctgtaatcg tatccgtcgt
    22561 ccggcgccag acggagctgc gcttgagagt ttccatcctt gaactcgaac acgaccgagc
    22621 cgccgttgac cacgctgagg aagtcctgca agtaggcggc gttgaaacct atttctgttt
    22681 cctcgccggc gtactcggtg gcgatgcttt ctctggcctc gccctcctcg gccgtctggg
    22741 agctgatctg caaggtgccg ggcatcaggt ggaggcggat ggcgtgggag cggtcgtcgg
    22801 ccatgagcgc cactctgcgt atcgcctgcc cgagcagcgt ggaatcaaac tcggccttgc
    22861 ggtcattgcc cttcggcatg accatgtcgt agttcgggaa ctggcccgcc aaagtgcgtg
    22921 aaatcaggag tcgtggcccc acctcgaaat aaatgtgatt ttcatccgcc ccgatgccga
    22981 catccccttc gttaccggac atcagcttgg tcaactcggc gagtgttttg cgggggatca
    23041 ggacgtctaa attttctttg ggcgcagtct ccccgaggtc cttacgctcg atgtaagcca
    23101 gacggtgccc gtcagttgtg atcatcttcg cgcccgactg attgagaata aacttcgccc
    23161 ctgagagcgt gtagcgcgat tcttccagcg tgatggcgaa gatggtgcgg tcaatgaacg
    23221 tcttgaagat gctgccggaa agtttgacgg ggggaatcac gaaactgggc acctcgggaa
    23281 aactctccct ttccacaccg gcaactcgaa agttcgagcg ttcgcacgtc accgcggccc
    23341 aattattctc ttccttacgg aaattcaccg gggcgtcagg aagcacgcgc gcgatttcaa
    23401 atagctttcg agcgcttaca cacatcgagc ctggcgattt gatgtccgcc tctgtctcac
    23461 agcggagcgt cacgtctaaa tccgtcccga gaaagcggat cgcattctcc ccaactgatt
    23521 cgatcagaat attcgagaga acagggattg tgttctttct ctcgatgatg ccctgaacaa
    23581 aacccaattc cttttggagg gcatttttgc tgattgtgaa ttccatttaa agctccttct
    23641 gatttcgact aatcgaatta aaaaatgctg tgtgtgtgaa ttgtagcgcc acgccttttc
    23701 ctacttatac taaaataaat atcttatatt tatacatagt agtagtagta gggcctgtgg
    23761 aaatgtggaa aagtgctttt aatggtgtga acaagaaact tagaatccac agcccctgtg
    23821 gaaatcgctg tggaaatagt tcctcggctg tgggtattat tatgtaatca agagttttcc
    23881 acatttccac aggccccaat ccggtatcac ctgtggaaat gtggaaaact tatagtcaaa
    23941 ttatagatat taaacagctt agatgaaatc gcccatgtgg aaaactttca aaggaaaatc
    24001 ttgcaactac ttgcaaagat tgtccataag gtcgctcacc accctgtgga aattctgatc
    24061 cctcgtaatg agagcctcga tcttgtctac agagtggata acggtcgtgt gatgcttacc
    24121 gccaaactca cgtccgattt cgggaaaact gtgtttggtg agccgtttgc acaggtacat
    24181 tgctacctga cgcggcacgg ctatctgtct cgaattgttc ttcgtcttga tgtcgtccgc
    24241 gcgtagacgg tagtgagcag aaacaacgcg ttgaatctgt tcgatggtga tgccggcggg
    24301 ttgattgtca tccgcgatgt ttcgtatcgc atcctgcgcg agcattttgg agatgggcag
    24361 gccgcgtagg gatgagatgg caatcagtcg caccagagac ccttcgagtt cgcgaatgtt
    24421 actcttcacc ttactcgcta taaaaaacgc gacatcgtcc ggaagcgaca ctccatcgag
    24481 atcggctttc cgcttaagga tggcaacttt agtctccaga tcaggaggtt cgatgtcagc
    24541 gattaagccc cactcgaagc gagaatgtag cctttcttcc agcgtgggta tctcacgcgg
    24601 cggacagtcc gacgtgatga cgatctgctt ctgagcatca tacaaggcat taaacgtgtg
    24661 gaaaaactcc tcctgagtgc gctccttccc ggccatgaac tggatatcgt ctatcagaag
    24721 cacatctatc gaacgatact tctcccgaaa cgtctgcgtc ttatcgtaac ggatggcgtt
    24781 gataagttca ttcatgaact tttccgccga gatgtaggaa aggcgcaggt aacgattatt
    24841 agttttaatt ttatggccga tggcgtgcat caggtgcgtc ttgcccagac ccaccccccc
    24901 gtatacatac aacgggttat aagtcttacc gggagcctca gcgacggcca gtgcggcggc
    24961 atgggcgaat tgattacaag agccgacgac gaaactttca aaggaacatt taggattaag
    25021 ggagttctcc agcggttcga tatcgacaaa ggtagtcgcc ccgggggtta acccgtcagt
    25081 ctcagccgcc tccaacaggg agaaaagatt cgaagacctg tccttctgag ccccctgatc
    25141 gggattattg ttcagaccga aattatcctt aacttcgtca cctccgaatg atttggaagt
    25201 ttccgactct tgaaccgtcc agtcaacttc ataagaagac aaattcagct cttccagtga
    25261 ggcgctaaaa acgtctgaat agtttgtgct tacccaatcc ttaaccacct ggttgggagc
    25321 gcgcaggtga agtttgtgtt ccgtctcatc gtacccctcg aaatgtatgg ggcggatcca
    25381 cgtttcaaag ctttggcggt tcagacgctt ctccacggcg tggagaatgt tgttccaaac
    25441 ctgactctca aacattgtac tcatgggttc tctccgctcc gaccaattcg agttcaagat
    25501 gaccgactca aaaacacaca ccacgcttcc ggctcagtta ccgttatcct ttgagccgtg
    25561 ccccggaaaa tattgtaagg acactctttc aatgccgctt tcacttacac tccagaaggt
    25621 tttccacaga cttttccaca agcttgtgga taatctcgga aacttaaagt gaagctttaa
    25681 gtgtattact ttcaattgtt tacagacggc aaagagcaac gaaacggccg ggagactaac
    25741 atatggtgta gacagatgca agagaattcc acaggcttgg attaattatt tgaatgtcta
    25801 tcaggattga tttaaaagtc cctgtctttt gagaaatacc gaatcaaaac attacacgtg
    25861 cctacaggtt ttgatagtga tggacagcta acaataacta tctatcagag ggtttattcc
    25921 ctctgaaatc gataaacaga tatttgctcc ctgcctattc ttgacaggaa agctgctcaa
    25981 agttagtctt atggttttta atcagaggag agaatgaata actatgccga agcgaacatt
    26041 tcagccgaac aatcgtcgcc gcgcgaagac acacgggttc agggcacgga tggcgaccaa
    26101 gaacggtcgt ctggttataa agaggcgtcg ggccaaaggg cgcaagcgcc tgactccgtc
    26161 ccactattaa ctagacgccg gcgcaatggg aacacgtatc tacgaagttc ttcagaattt
    26221 cgccccgtct acgaccgcgg cgcgcgcttt aatgggacgt tgatgacggt cttcgtactt
    26281 cgcagcggcc acggctctca ccgattcggc atcacggcct cgcgaaaaat gtcacgcagc
    26341 gccgttcaac gtaaccgcgc caagcgcctt ctccgtgaaa cgtttcgttt gtgcggcgac
    26401 gatctgagca agctgcaaaa taagtatgac tgggtggtta atgccagacg gtcgcttctg
    26461 agcgttaaat tgccgcagcc attgatggag tttcgggaaa ttatcgcccg tgtcggcaga
    26521 gatgaacgcg gggcggcttg agccgggttt ttaaggcggt gaaagcaata atcttgggac
    26581 tgttgagatt ttataaagcc gcggtctcgc cactgctgcc accttcctgt cgcttcatcc
    26641 cgacctgttc tgagtatgca cgagaagccg tcgaaagaca tggcgcgctc agaggcactg
    26701 ctctgggcgc acggcgactt ttacggtgtc atcccttcca cccgggtggc tatgatcccg
    26761 tcgagtagtt gtttccagct ttcttaaata ttattgcggc cgagcattcc cctttgagtt
    26821 gttgactaca tggaacagaa acgcctcttt cttgccctga ttatttcgac agtcatcctt
    26881 ctcggctgga gctatttttt tcaacccagc accccccagc gcaattcaaa cgcttctcaa
    26941 gcgccgcccg ccgaatctcc acaggcttcc aatcagcaac ccgacactgc tccgcgaccg
    27001 gccgagcctt cactcactcc gggcggagac gtgaaggtct ctgaagacac agtccgaccg
    27061 caggtgattg ttgtctcgac gcctctctac aaggtggaga ttgataatcg cggcgccgtg
    27121 gtcaagagtt ggataatcag acaaaacaag gacacgggtc gcccgcttta ctcgattgcg
    27181 ggtgataagg agaagcggat tgatcttgaa ctcgtttccc ccgaaggact cagtcgcggc
    27241 caggcaccct tgagtcttga aatagcaagg cttggcctga atgacggctc aaccccacaa
    27301 ctccttcccg ctttaaacga cgcactgaag aacagatatt tcgaggtgac gggaagaaac
    27361 ttcgcgctgg ggacagcgag tgcggaaggt ggcccgatca atttgaacat cgagtcggga
    27421 gagccgaaaa agtatcttga tttcaaaatg gtcgagccgg acactggtct tgaagtggtt
    27481 aagagtctgg ccttcgatcc gcaaagttac acgactgatg tcgaggtaaa agtaaatcgg
    27541 cgcggacaaa ctgttcctga cgtgaggatg ctcatcggcc cgagcacggg cgaccaaggg
    27601 gtgccgcttt atacgttcta ctcggtcgcg ccgcagggcg tcgctgtggc caaaggcgac
    27661 gcagttagga tttacgcgac ggacgtacac aagcagaagt cggcgcaaga ccctgttaaa
    27721 ggttataagc ccattgaggg gccggttgac tgggccgccg tcggcgacac ttattttgca
    27781 atggcgctcg ttttgcccag acccgccgag aaggttgagt tccttactga agaatatcag
    27841 cacacgtccg gcgatacgaa ggaacaaaga tttctcatct ccggtttagt ccccgtcccc
    27901 accgatggct ccgcaatgag tctctacgtc gggccgaagg atcacttcgc gctggacgac
    27961 acgagtcgga ctttgagtca ggtcgccgga cgtgaggtcg atctcgagca gttgattgat
    28021 tacgggtggc tcggtttcac gcggcccatc gccgtccaac tcctgaaagc catcaagttt
    28081 ctcaacgacg tgacgggcaa ttacggcgtg gcgatcattc tgttcacgat catcatctac
    28141 atgctcttct tcccgctcaa gtggcgctcg accaagtcga tgaagaaggc acagaagcac
    28201 gcgccacgaa tgaaagagat tcagcagaag atcaaggaac tgaagcctaa cgatcctcgc
    28261 ttgaaggaat tgcagatgga acagctgcgt ctgatgaagg aggcgaaccc gctcggcggg
    28321 tgcctgccgc tcctgattca gatgccgttt ctcatcgcgc tctacagcgc catcacgatc
    28381 tcaattgatt ttcgtcaggc gtcttttctc tggatgcccg acctttccgc ctccgacccg
    28441 attagacttc tgccgatcct gatggccggg tcgatggtga ttttgcaatt catcacaccg
    28501 gccccgtcgg ccgatccgct gcaacgaaaa atgatggcgg tagtgctgcc ggtgatgatg
    28561 ctctacatgc tctggagcgc gccgtcgggt cttctggttt actggctcgt cggtaacatc
    28621 gtcgggttcg gacagcagtt cgtcatcaat cgaatggtga agtcggaaga cgacgaagag
    28681 ccgccgggga agaagaaaga agtaaaccct aagaagccca aactcggcgc cacgagcgtg
    28741 tcgcaggcgt agacgcgcgg cggcttgaac gcgcggcgca cgatgaaacg atgaaagaaa
    28801 tcttcaatca atcacaggat tttctgaatc aagttttcac cagcgtgaga ctggatttgc
    28861 aagcgtctgc cgcggagact gagggcgggt gcgtgttgaa tatcgacggg gccgacgccg
    28921 ctctccttcg cagcgaaggc ggagaactcc tggacgccct cgagcatctg gttaatcaat
    28981 ctttcgcgcg tggtcttgaa ccgggtgaac gcctcgtctg tgacgtggaa gattttcgcc
    29041 tcgtgcgcga aacagagttg cgtgcgatgg cgcgtcatgc cgccgacagg gttcgcgcgt
    29101 caggcgttcc attcatgttc ggtcccatga atgcgaacga gcgacgcatc attcacctag
    29161 aactcgctaa tgaagagcaa ctccacacgg aatcagtcgg cgagggtagc gaccgccgcc
    29221 tcaaagtaag tcttaagtct ccctccaggc agtaagcgtt tcatcgtctt cgaagcgact
    29281 cccggcgcgc ggggtgaact ctccaatagt gcaagcaaca gacaccatcg ttgctctctc
    29341 cacgccgccc ggccgaagtg gcatcggagt catccgcctg agcggcgcat cctcactcga
    29401 cctcctcaaa attctcgtca gagatcaaca tttcgcgccc gaacctaatc gcgttactct
    29461 aaaaggaatt tacgttcctg acaggggcga attattagac cgtgctctca tcacttactt
    29521 caaatctccg cattccttca cgggcgaaga tgtggtcgag ttgagttgcc acggctctcc
    29581 gccactcctt ctgcatgtta tcgacgcgct cctcagctcc ggcgcacgca ccgccgaccc
    29641 gggcgagttc accttacgcg cgctctcgaa tgggcgcatg aacctgtgtc aggctgaagc
    29701 cgtgcgtgat ttaatcgacg cgcaaacatt ggccgccgtg caacaggccg cgcgacagct
    29761 caacggcgaa ctctcttctc gtcttcaacc attcaaagac actctgctgg aaattatcgt
    29821 ccctctggag tcagctttag aatttgttga ggatgatcta cccgaagaca taacttctca
    29881 tctcgtccaa agcctcgaaa ccctcatcac ccgcttgaga gccgtggttg atacttttca
    29941 gtccgggcgc ttgttaaagg aaggtcttaa aatcacgctg accgggtgcc caaatgtcgg
    30001 taagtccagc cttttcaata gtttgctgat gcaagagcgc gctatcgtaa ccagcattcc
    30061 gggcaccaca cgtgatagtc taagtgaact catcaacatt aacggaattc ctgttttgtt
    30121 aacagatacg gccggtctga ggtcttccgc tgacgtgatc gaacagcttg gcatcgagcg
    30181 aactgaacaa gctgttgtgg atgctgattt tgtggttgtc gtaattgatg gttctcagcc
    30241 gctaactgat gaagataaag acgttcttgc aaaagccacg aaaaggaatt atcttgtggc
    30301 tttaaataaa agtgacctca aaagctttaa tgtcagcaat cttcaggata caaataatca
    30361 cgcgcccgtt atttctgttt cagccaaaac gcgagatggt cttgacgagc taattaatgc
    30421 gataatggag cctttcacag cacacggaat ccctgaaact gatttcctta tcacgaatgc
    30481 gcgccattat gatttgctaa aacgcactat cagttcactt caatcctcgc aggaattgct
    30541 gaaacggggg tcaagtgaag aaatcactct caccggactc cataatgcac tgcgattttt
    30601 gggagaaatc actggtgaaa ccacccccga agatatcctt actcaaattt tctccacctt
    30661 ctgcatagga aaataatcat gggttacgac gaagtttttg atgtcatagt gattggcgcg
    30721 gggcacgcag gttgtgaagc cgcttcggcc gccgcacgga tgggttccca gacagctctc
    30781 gttaccctaa atcttgatat gattggacaa atgtcctgta acccggcagt gggcgggatt
    30841 gctaaggggc atctggtacg tgagatcgac gctctcggcg gcattatggg ccacacgatt
    30901 gatcgtaccg gcattcaatt tcgcctctta aatcgttctc gagggccggc agtgcgtgcc
    30961 ccgcgcgccc aggctgatcg gtctttttat cgtaccgaga tgcgtcggac tcttgagaat
    31021 acgcccaacc ttcatctgat tcagggtctg gttattgatt ttattgttga taaggatcac
    31081 gtccgcgggg ttgagcttca agacacacgc agacttcgtg ctgatgccgt catcgttgct
    31141 acaggcacgt tccttaacgg actaatccac acaggtcgcc gtacttacac cgccggtcgt
    31201 gcgggagaac ctgcctctat tgaactcgcc gagagcctca agaaacttgg atttcctgtc
    31261 ggccgcttga agaccggtac tcccccacgc cttgacgggc gtactattga atgggatgtg
    31321 tttgagccac aacccgcgga cgaacgtcca gttcccttct cgttttcaac cgaaagtatt
    31381 gagcagccgc aggttaactg ttttattggt tatacgaatg aaacagttca taaaacgatc
    31441 tctgataatt taagcgaatc ccccctttat tccgggcaaa taaaaggagt tggaccacgc
    31501 tactgtccat ccatcgaaga taaaattgtc aaatttccca gtaaatcgcg gcatcaatta
    31561 tttctggagc ctgaaggtta tgatacccat gaggtgtatc ttaacggctt ttcaacttca
    31621 cttccttctg accttcaaca tgaattagtt caccagattc gcggccttga agaagcacgg
    31681 attattcggc ctgggtatgc tatcgagtat gattttgttg accctcgtga attgaccccg
    31741 tttttacaaa cctctcgctt taaagggctt ttcttcgcgg ggcaaataaa cggcaccacg
    31801 ggctatgagg aggctgcatg tcagggtttg attgcaggta ttaatgcctc attatttgtt
    31861 caggggcggg aaggttttag acttgggcgt gaagagtcct atattggcgt tttaatcgac
    31921 gatttgatca ctcatggcgt tgacgaaccc taccgaatgt ttacttcacg cgccgaatac
    31981 cgtctgcaat tacgctacga cacggcagat gcccgactca cgtcttatgg ctatgaacta
    32041 ggactcatgg gtgatgatgg atggcagaaa tttttacgcc gcagaaaccg catttcccaa
    32101 ctccgtctaa gccttgaatc cactaaattt aatcgtacgc acccagaata ttcttatatt
    32161 tccacccttc tagataaaga tttaggtgat tcattctccc tttcttcctt tgtacataat
    32221 cctggtattg ctccggaagt tatctgccag tttttgcctg cgaatctaca aaccggcact
    32281 tcaatttcag aacttgagtc ggctttagcc gatgttcttt atgccgggta tatttcattt
    32341 caacagaata atatcgagcg cctataccat agcgattcag ttcccatccc gaataatttc
    32401 aaattcggct tagtaagcgg cttgtctaat gaaatggtcg agcgtctaga gcgcaccgcc
    32461 ccgcaaacct tcgggcaagc ccggcatatc ccaggactta ccccgggcgc cttgacgact
    32521 ctgctctatc acctcaaact taactagatc gaacatcctc aatgtttcac gtgccacata
    32581 tcgcaaattg tgacacgtga aacataaaac tgttcgctgt aggtgcaaac tctctggcag
    32641 agttgtaact tttgtgatag tttgtaacca cgttacacag gcgctgccga ggtcgcgaat
    32701 ggcaaaaatt attgcggtgg cgaatcagaa aggcggggta ggtaaaacta caacggccgt
    32761 taatttagcg gctgctcttg ccacttctga gttgcgtgtc cttctggttg atgcagaccc
    32821 gcaggcgaat gcgacttcgg gcgtgggcat tcaaagaggg agtcttcgcc ggagtctgta
    32881 tcatatactc gtccttgatg aacgggtctc gaatgta
//
LOCUS       DASVES010000145         5912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_911554, whole genome shotgun sequence.
ACCESSION   DASVES010000145 DASVES010000000
VERSION     DASVES010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911554"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..751)
                     /locus_tag="VF240_05570"
     CDS             complement(23..751)
                     /locus_tag="VF240_05570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174732.1"
                     /translation="MTTLTQNAQAAYEAWHERLDVDVESDAPWHRLVKQHLEDGRDLS
                     GRRVLEIGCGRGGFACWLASRTDGPIEVVAADFAESAVTKGAAFARSRGLGKIKWEVH
                     DIQAIDHPDASFDTVVSCETVEHVPDPRRAIQELARVLRPGGRLFLTTPNYMGTLGLY
                     RGYMRLTGRRYTEEGQPINNFMLLPRVRSWVAQTGLRVAAVDAVGHYLPWPGRPPIEV
                     PALNDPRPVMRWLGLHSLVVGEKK"
     gene            complement(748..1947)
                     /locus_tag="VF240_05575"
     CDS             complement(748..1947)
                     /locus_tag="VF240_05575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7174733.1"
                     /translation="MRVLLASYVDDNRWSGMGKWSHCMAEGLARLGHQPTLWFADEFT
                     FARRFGRFAVLLFPAALAIRLWRRRKHFDAVVIHEPSGFWYGMLRSVVRSLPPMVSMC
                     HNVESKNYELLLDADRRALAVVPRGTRVKAPLIRLWQSDGAIRLADHVVCLSDVDRNY
                     VINRLGRAPDKVTVMHNGVDAKFFHARAEKSFGHSVLFVGGWWDVKGRRVLPLLWSQV
                     RERLPEARLTIVGSGRAGNEVVADFAERDRLSVNVIPRIGDESEMISQYAAHDVFLMP
                     SLSEGSPLALLEAKAAGLPIAAARVGGVPDVIAHEEHGLLFDALDPSDGAKQVCRLLG
                     DKITAQRLGRAAQEHVRQFTWAAATQTLATAIESVLEKADLRRGLSVPGKDDYESEAC
                     AGRVLSK"
     gene            complement(1919..3262)
                     /locus_tag="VF240_05580"
     CDS             complement(1919..3262)
                     /locus_tag="VF240_05580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7174734.1"
                     /translation="MRIAVVNWSRRRAGGVETYLGGVIRELLRSGHEIAFWHECDKPA
                     DRERIALPEGIPVWSVETLGARGALDALKEWRPDLLYSHGMLDPALESATLELAPAVF
                     FAHSYYGTCISGSKTFRRPIVRPCGRSFGWQCLVNYYPRGCGGRSPVTMFREYWRQSA
                     RLDLLHRYDAIVTHSGHIQEELIKHGLSVRSAYKFPYYVNGAEQALMAGDLAATAYAR
                     NGEANANGDALGGRDGKLADERGRHLLFVGRMDALKGGSVLVDALPRVRKGLGVPLRV
                     TFAGDGPARRAWERRAARVEGRNEGLHFEFPGWLGGERINALLGACDLLVVPSLWPEP
                     FGLVGPEAGLRGVPVAAFGVGGIPDWLIDGVNGRVAPGDPPTVAGLSEAIVDCLRDPV
                     RHAEMRREAVRLAQKYNMKNHLAALLDVFEGVVRREREKYSISGGQDARPVSVLR"
     gene            complement(3263..4522)
                     /locus_tag="VF240_05585"
     CDS             complement(3263..4522)
                     /locus_tag="VF240_05585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7174735.1"
                     /translation="MRSIAFENSSALGEWHVVTPEYPPQTGGVSDYARLIADGLAADG
                     CVVQVWCPSLRDGDGDRSVVEPSPPRVHVRRELGRFSPGDLRRAGLLLDARPGPRRLL
                     VQYVPHGYGYRSANVAFCAWLLKRARVAGDRVEVVIHEPFLEFARWSWKRNAAAAAHR
                     VMAAMLLLAARRVWVTTPAWGELLRPYALGRPVDFRWLPAPSTVPVLEDAALTSDVRG
                     RYSVAAAGASLVVGHFGTYSAHVFELLEQTLPALLARRPEVSALLMGRGSAEARARLI
                     ELHPELGARLHATGQLPPRELSAHLAACDLMLQPFPDGVSCRRTSVMAALAHELPVAT
                     TRGRFTEPLWAESRAVALAPAGDTAALTDTAARLLADGRARASLARAGRSLYRAKFDV
                     SNIVAALRGGDSPGRVGASLLTEEVKA"
     gene            complement(4519..5361)
                     /locus_tag="VF240_05590"
     CDS             complement(4519..5361)
                     /locus_tag="VF240_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7174736.1"
                     /translation="MSLIQRPARMVSGVLGHESWLVRRLRPVYESVLDLSAGGRGIPW
                     EINGVTYRVDPRQRHRLGQNYDAPVADFIRARVRPGSVCVDVGANVGVYVLQFAHWAG
                     PSGRVVAFEPNPGAREVLARHIEMNDLGARVEVAPAAVGASSGSATMYASGADGMSRL
                     GAPNVLISERVSELTVPVVTLDDFCRERALEPDWLFLDIEGFEIAALEGARNLIARRG
                     AALNVVVEMHPNVWDSAGTTRADAEALLEGLGLRAVALTGQTDPLGEHGIVYLERNGE
                     TRDF"
     gene            complement(5358..>5912)
                     /locus_tag="VF240_05595"
     CDS             complement(5358..>5912)
                     /locus_tag="VF240_05595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7174737.1"
                     /translation="REARAVFVGRLLPHKGIDDLIRALPDGVALDIIGQPYSEEYLRE
                     LKRLAEGRPVRFRHDCDDAALVRAYRGALCVVLPSVYRSAYGQGHESKAPELLGQTLI
                     EGMACAAPAVCTDVASMPEVVEDSVCGFVVAPNDPASLGAAIAWLRDHPREAGEMGRA
                     ARLRVLEKFNWPAVVGRCLEIYAR"
BASE COUNT         1030 a         2136 c         1834 g          912 t
ORIGIN      
        1 aacagcacac gcaaacggcg actcatttct tctcacctac aacgagcgaa tgtagaccca
       61 gccagcgcat gacgggcctc gggtcgttga gcgccggcac ctcgatgggc gggcggcccg
      121 gccacggaag gtagtggccg acggcgtcca cggccgcgac tcttagaccc gtttgtgcca
      181 cccaactcct cacgcgtggg agcagcatga agttgttgat gggctgaccc tcctccgtgt
      241 agcggcgccc cgtgaggcgc atgtagccgc gatagagtcc gagcgtgccc atatagttcg
      301 gcgtcgtcag gaagagacgg ccgccgggcc ggagaacgcg cgctaactct tgaatcgcgc
      361 gtcgcgggtc gggcacgtgc tcgaccgtct cacatgagac tacggtgtcg aagctcgcgt
      421 cggggtggtc gatggcctgt atgtcatgta cctcccattt gatcttgccc aaccctcgcg
      481 agcgggcgaa cgccgcgccc ttcgtgacgg cgctctccgc gaaatccgcg gcgaccactt
      541 caatcgggcc gtcagtcctg ctcgcaagcc agcacgcgaa gccgccgcgc ccgcacccga
      601 tctccagcac gcgccggcca gagaggtcgc gcccgtcttc gaggtgctgc ttgacgaggc
      661 ggtgccacgg cgcgtccgac tcgacatcca cgtccagccg ctcgtgccac gcttcgtagg
      721 cggcctgcgc gttttgagtg agagtggtca tttgctcaac acccggccgg cgcaagcctc
      781 actttcgtaa tcatctttgc ccggcacgga gaggccgcgc cggaggtcgg ccttctccaa
      841 cacactttca atcgccgttg ccagagtttg cgtggcggcc gcccaggtga attgccggac
      901 atgttcctgc gctgcccggc cgagacgttg ggcagtgatc ttgtcgccca gcaaccgaca
      961 aacctgcttt gccccgtctg acgggtctaa cgcgtcaaac aaaagcccgt gctcctcatg
     1021 tgcaattaca tcggggacgc cgcccacgcg tgcggccgcg ataggcaggc cggccgcctt
     1081 cgcttccaac aacgcaagtg gcgacccttc cgacagcgag ggcatgagga atacgtcgtg
     1141 tgccgcgtac tgtgaaatca tctccgactc gtccccgatc cgcgggatga cattcacgga
     1201 cagacggtca cgctccgcga aatccgcaac cacttcattc cccgccctcc cactcccgac
     1261 aatcgtcagt cttgcctccg gcagtcgctc gcgaacctgc gaccagagca aagggagcac
     1321 tcgacggcct ttcacgtccc accacccgcc gacgaagagc acgctatgac cgaacgactt
     1381 ttcggcccgc gcgtggaaga acttcgcgtc aactccattg tgcatgactg tgactttgtc
     1441 aggtgcgcga ccgagacggt tgattacata attgcggtca acatctgaca gacagacgac
     1501 gtggtcggca agtctgatgg ccccgtctga ctgccacagc cggatgagtg gtgctttaac
     1561 acgcgtcccg cgcggcacaa cggctaacgc gcggcggtct gcgtccagca ggagttcata
     1621 atttttactc tcgacgttgt gacacatcga aaccatcggc gggagggagc gcacgacgct
     1681 cctcaacatg ccgtaccaga agccgctcgg ctcatggatg acgacggcgt cgaagtgttt
     1741 tcttcgtcgc cagagtcgga tggccagcgc ggcagggaat aacagaaccg cgaatcttcc
     1801 gaatcgacgc gcaaaggtaa attcatccgc gaaccagagg gtcggctgat ggcccagccg
     1861 tgccagccct tcggccatgc agtgagacca cttgcccatc ccgctccagc ggttgtcatc
     1921 aacgtaggac gctaacagga cgcgcatctt gacctccgct gatggaatat ttttcgcgtt
     1981 cgcgccgcac cactccctca aacacgtcga ggagggcggc gagatggttc ttcatgttgt
     2041 acttctgcgc cagccggacg gcctcgcgtc tcatctccgc atggcggacg gggtcgcgca
     2101 ggcagtccac gatggcctcg gagagtcccg ccacggtggg cgggtcgccc ggtgcgacgc
     2161 gcccgttgac gccgtcgatc agccagtcgg ggatgccgcc gacgccgaag gccgccaccg
     2221 gcaccccgcg caggcccgcc tcggggccga ccagcccgaa cggttccggc cacaggctcg
     2281 gcaccacgag caggtcgcac gcgccgagta gcgcgttgat gcgctcgccg ccaagccagc
     2341 cgggaaactc gaagtgcaga ccctcgtttc tgccttcgac acgcgccgcg cgccgctccc
     2401 acgcacgccg ggcggggccg tcgcccgcga acgtcacacg cagcggcacg cctaaccctt
     2461 tcctcacacg cggcaaggca tcgacgagca cgctcccgcc cttgagtgcg tccatgcggc
     2521 cgacgaagag caggtgtcgg cccctttcgt ccgcaagctt gccgtcgcgc ccgcccaacg
     2581 cgtcgccgtt cgcgttcgcc tccccgttac gtgcgtatgc cgttgccgcg aggtcgccgg
     2641 ccatgagcgc ctgctcggcg ccgttgacgt agtaaggaaa cttgtaggcg ctcctgaccg
     2701 agagtccgtg tttgattaac tcttcctgaa tgtggcccga gtgggtgacg atggcgtcgt
     2761 agcggtggag caagtcgagg cgcgcggact gtcgccagta ctccctgaac atcgtcacgg
     2821 gactccgccc gccgcagccg cgcggataat agttaacgag acactgccag ccgaagctgc
     2881 gtccgcacgg gcggacaatg ggccgcctga aagtcttcga cccgctgatg catgttccgt
     2941 aatacgagtg tgcgaagaag acggccgggg ccagctccag cgtcgcggac tccaacgcag
     3001 ggtcaagcat cccgtgcgag tagagcagat cgggtcgcca ctccttgagc gcgtccaacg
     3061 cgccgcgcgc accgagcgtc tcgaccgacc acacggggat gccttccggg agcgcaatcc
     3121 gttcgcgatc cgcgggcttg tcgcactcgt gccagaaggc tatctcgtgg ccggagcgca
     3181 gcaactcgcg gatgaccccg ccgagatatg tctcgacgcc gcccgccctt cgccggctcc
     3241 agttgaccac cgcgatacgc atctacgcct ttacctcttc ggtcagaagc gacgcgccga
     3301 cgcgccccgg cgagtcgccg cctcgcaacg cggcgacgat attggacaca tcgaacttcg
     3361 cgcgatagag actgcgcccg gcacgcgcga ggctggcgcg cgcgcgccca tcggcgagca
     3421 aacgcgcggc cgtgtcggtg agcgcggccg tgtcgccggc cggggcgagc gcgacggccc
     3481 ggctctcggc ccacagcggc tcggtgaagc gcccgcgcgt ggtcgcaacg ggcaactcgt
     3541 gcgcgagcgc ggccatgaca ctcgtccggc ggcaactcac gccgtcgggg aacggctgca
     3601 acatgaggtc gcacgccgcg aggtgcgccg acagctcgcg tggtgggagc tgtcccgtcg
     3661 cgtgcagacg cgctccgagt tcgggatgaa gctcaatcag gcgcgcgcgc gcctcggcgc
     3721 tcccgcggcc catgagcagc gcgctgactt cgggtcgtcg cgcgagaagc gccgggagcg
     3781 tctgctcaag aagctcgaag acgtgcgccg aataagtccc gaagtggccg acgacgagtg
     3841 acgcgcccgc cgcggcgacg ctgtagcggc ctcgcacatc acttgtcagc gccgcatctt
     3901 ccaacacggg aaccgtactc ggcgccggca gccagcggaa atctaccggg cggcccagcg
     3961 cgtatggccg tagcagctcg ccccacgccg gcgtcgtcac ccagacgcgc cgcgccgcaa
     4021 gcaaaagcat cgcggccatc acgcgatgcg ccgcggctgc cgcgttgcgc ttccaagacc
     4081 agcgcgcgaa ctccagaaac ggctcgtgga tgaccacctc cacgcggtcg cccgcgacgc
     4141 gcgcgcgctt gagaagccac gcgcagaagg cgacgttcgc cgagcgatag ccgtaaccgt
     4201 ggggcacata ttgcacgagc agccggcgcg ggccgggccg cgcgtcgagc agaagccctg
     4261 cgcgtcgcaa atctcccggc gagaaacggc cgagttccct tctgacgtga acgcgcggcg
     4321 gagaaggctc gacgacgctc ctgtctccat cgccgtctcg cagggacggg caccacacct
     4381 gcaccacaca gccgtcggcc gcgagaccgt cagcgatgag gcgcgcgtag tcgctgacgc
     4441 cgcccgtctg cggcggatac tccggtgtga cgacgtgcca ctcgccgagc gcggaactgt
     4501 tctcaaaggc gatgcttctc aaaagtccct cgtctccccg ttgcgttcaa ggtacacgat
     4561 gccgtgctcg ccgagcgggt cggtctgccc ggtgagcgcc acggcgcgca ggcccaaacc
     4621 ctccagcagc gcctccgcat ccgcgcgcgt cgtccccgcc gagtcccaca cgttcggatg
     4681 catctcgacg acgacgttca aggccgcgcc gcgccgcgcg atgagattgc gcgccccttc
     4741 gagcgccgcg atctcgaatc cctcgatgtc gaggaacagc cagtcgggtt cgagcgcgcg
     4801 ctcccggcag aagtcgtcga gcgtgacgac ggggacggtt agctccgaca cccgctccga
     4861 aattaaaacg ttcggcgcgc ccagccggct catcccatct gcgcccgacg cgtacatcgt
     4921 cgcgctccca ctactcgcgc cgaccgccgc cggcgccacc tccacacgcg cgcccagatc
     4981 gttcatctcg atgtgtctcg ccagcacctc gcgcgcgccg gggttcggct cgaacgccac
     5041 gacgcgaccg ctcggccccg cccagtgcgc gaattgcaaa acgtagacgc cgacgttggc
     5101 ccccacgtca acgcagaccg agccggggcg gacacgcgcg cggatgaagt cggccaccgg
     5161 cgcatcgtaa ttctgcccga gccggtgacg ctggcgcgga tcgacgcggt aggtcacgcc
     5221 gttgatctcc cacgggatgc cgcgcccgcc ggccgagagg tcgagcaccg actcgtagac
     5281 cggacggagc cggcgcacca gccacgactc gtgccccaac acgcccgaca ccatccgcgc
     5341 cggtctttga atcaaactca tcgcgcgtaa atctccagac agcggccgac gaccgccggc
     5401 cagttgaact tctccagcac cctcaaacgc gccgcgcggc ccatctcccc ggcctcgcgt
     5461 ggatggtcgc gcagccacgc tatcgccgcg ccgagcgacg ccgggtcgtt cggcgcgacg
     5521 acgaacccgc acacactgtc ttcgaccacc tccggcatac tcgccacatc cgtacacacg
     5581 gccggcgcag cgcacgccat cccctcgatg agcgtctgcc ccagcagctc cggcgccttc
     5641 gactcgtgtc cttgcccgta ggcgctccgg tagacgctcg gcagcaccac gcacaacgcc
     5701 cctcgatagg cgcgcaccaa cgccgcgtcg tcgcagtcgt gccggaacct gaccggcctg
     5761 ccctcggcca gccgcttcag ctctcgcagg tactcctccg agtagggctg cccgatgatg
     5821 tcgagcgcca cgccgtccgg caaagcgcgg atgaggtcgt cgatgccctt gtgcggcagc
     5881 agccgtccga cgaagaccgc ccgcgcctcg cg
//
LOCUS       DASVES010000146        10317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_993814, whole genome shotgun sequence.
ACCESSION   DASVES010000146 DASVES010000000
VERSION     DASVES010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993814"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..811
                     /locus_tag="VF240_05600"
     CDS             95..811
                     /locus_tag="VF240_05600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174738.1"
                     /translation="MTEGRSVRPVEEGIYTVLAEGARAAHLYDRRAGVYDALVGTRLY
                     NRVMWGASPEDYAAFARRAVDARAAGWMLDAACGSMLFTARAYVETGRRLIAFDQSLR
                     MLRRARERLRGFGGGRPCANVLLLQADLSDPPFRPASFQTVLCMNVLHQFADADGLIP
                     KLAGLLADGGQLHLTSLVSNRRFIGDRYLDALHRAGEFVRPRRSSEFEKLLRDALSQG
                     VSYTTRGNMAFATWERPTPA"
     gene            818..1636
                     /locus_tag="VF240_05605"
     CDS             818..1636
                     /locus_tag="VF240_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174739.1"
                     /translation="MTTNHMDAARHPGHGAEVERLYRDIAVETRYGHYVRIPERICRC
                     LDYFGVRHERAAVAERLHSYYLFIGVVDDAIDASRAGAGESVLKRLEASAPSFDEETR
                     RTRTGLLTEVLKTHIGPEVYTPARRKLEELYGAVVGERGAGTLGAYIERRKTVGRLTT
                     EVSYLLIAPLLEGGRDGLLGFLRRVGEVGCLVDSVVDLRADERAGLLGFRPTARDRLR
                     LAGLTVYEGLRMWSEHPRLTRLFLEAVGDVLLDRLRARRPQPAADGRAQLEVEY"
     gene            complement(1927..2841)
                     /locus_tag="VF240_05610"
     CDS             complement(1927..2841)
                     /locus_tag="VF240_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7174740.1"
                     /translation="MMSTNLSTRRAIFCALAVVVCAAFSHAPRAEGAGEGARVGRSRF
                     ARFENMRVHYADAGKGSEALVFVHGWTCNLDFWRMQTPVFAAKSRVLAVDLPGHGQSD
                     KPQQVAYTMDLFARAVEAVMRDAGVKRAVLVGHSMGMPVVRQFYRRYPEKTLALVNVD
                     GGLRPFAPRERMEQFIAPLRGSDYKENAARMVDGMLQPVKSEALRAEIRAAMLATPQH
                     VAVSAMDGMMEQSVWKEDQIKAPMLAVLARSPFWPADNEQFFRRIAPELDYRMWDGVS
                     HFLMMDKPDEFNRALGEFLASRKLLAKK"
     gene            2910..3275
                     /locus_tag="VF240_05615"
     CDS             2910..3275
                     /locus_tag="VF240_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174741.1"
                     /translation="MEHAPGSLPEWEESDLTFPFTLKAAFGKEKYVFNGLGDGGVLTN
                     FKDSEHRQSCGDALIDILLHRRYIMCEQNAAFRRGPFEHFRIPFAQQSGILNTHDVQI
                     TSAAQQPTQDTIIEVFVSR"
     gene            complement(3515..3832)
                     /locus_tag="VF240_05620"
     CDS             complement(3515..3832)
                     /locus_tag="VF240_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX7174742.1"
                     /translation="MSQGTTTVPLSKDQGGVLRVAGTRVSLDSVIYAFDEGSTPEEIA
                     QAFPTLDLAAIYSVLGYYLQNRAEIEQYLEQRKVERQELKKELEARFNPHGLRERLLA
                     RKQ"
     gene            complement(3884..4027)
                     /locus_tag="VF240_05625"
     CDS             complement(3884..4027)
                     /locus_tag="VF240_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174743.1"
                     /translation="MTSFCITIDRDSETELRDLYGEVGYTIIDDVLSLPERMPAIYRR
                     LTT"
     gene            complement(4103..4435)
                     /locus_tag="VF240_05630"
     CDS             complement(4103..4435)
                     /locus_tag="VF240_05630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX7174744.1"
                     /translation="MTVGELRWVAMPARGGHAQTGRRPAIIVQQASALPTVLLIPLTS
                     QQDALRFAATVLVEPDEHNGLRQPSVALVFQLTAVDQKFVGARLGRVSGAALKEIWLA
                     FDELTGRT"
     gene            complement(4432..4677)
                     /locus_tag="VF240_05635"
     CDS             complement(4432..4677)
                     /locus_tag="VF240_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7174745.1"
                     /translation="MSVIEKLIVEGGGRITLPSDVLDRYGLAEETIVRIIETQKGILL
                     IPLTDEPMGEALASELREWQAIGNESWEMFPFEETEP"
     gene            complement(4749..7976)
                     /locus_tag="VF240_05640"
     CDS             complement(4749..7976)
                     /locus_tag="VF240_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174746.1"
                     /translation="MKTQDHGLNTKARDERPEGAEPLDKALRERLRSVRSFGERRTIL
                     GLVREIGNLPVESARAALEVGAGLAGLSLRVAVEFLRAAPDAAQVLADEELRAWGEMG
                     RRLAMADLDTGAEFFAAGVSDLEGVPREALPVLFLVCARQSTLSTSIALETFRGAAGL
                     ARAVGDAGLLRSVYEVAVEIARRSAKHSAEFLEATPRAVEALGAVGGGAGGAAATKRA
                     GVRLAEVFATRAGGIASDMWAALPDSVGGLGERDALRLFRCAEDFLDRGGAAALHVFV
                     AGGGVLRALPETFADWTDLLREVAEQSNAALVALARASPAFFRAVVERTNRERASELA
                     GRVVRASREVARVDAEAAICCFRSGPRALRSTSIEQFEHWAAEGLTDERATSARARRS
                     YYALETRRSNERLTGGGAGLALEEVAQTLRLYVEGLTGRSVEVAPLSAVPEESRIGDG
                     RTIHLPSSVAEFDDEARDFRLYKVLAAHAAGQIEFGTHARDSASLRAAYSSLADLYAP
                     EHADALDAFALDGYINDIPAGERALPPEEEARLARERRRELPAAADYRAALALFPQKG
                     LAARIFGTLENGRIDRRLRHAYRGLARDLDLVRAHLRENRPRVAGLPIALVPFELLFQ
                     ITLCGGASDDARASYGQIVSELESIVIDYLTSPDAEVGDTLMATSRVYTLFQAVVPDM
                     SERQQDAPEQSGDAESSGAQDRLPQETEEGEEERERRRDEARRRDARELFNAWAASEV
                     SDADGEEAFTGTERWTSAESSEQELEPDEVAYSYDEWDRELSDTRVGWCRVIEKKVRR
                     GDRAFVEQTRTRYRGVISSIRHQFQLMKPENLRRIPNELDGDDYDLSAVIDYVMDRRA
                     NDGQQSERIYTKRLRRERDVAVSFLLDQSSSTARTIGRHPLQPYTHPGRRIIEVEKEG
                     LVLMSEALEAVGDSYSIYGFTSEGRRNVKFYVVKDFDETYTEETERRIGGINYQHNTR
                     LGAAIRHAAARLQQRDARTRLLIVLSDGRPYDHDYGDARYAREDTREALRHARASGIT
                     PFCITIDRDSETELRDLYGEVGYTIIDDVLSLPERMPAIYRRLTT"
     gene            complement(8350..8565)
                     /locus_tag="VF240_05645"
     CDS             complement(8350..8565)
                     /locus_tag="VF240_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174747.1"
                     /translation="MTELLEKAFTAASKLSEEDQNALATMLLKDLAAEELWDGALANS
                     QDKLAALADEALAEFGRGETKPLDEDL"
     gene            complement(8663..9469)
                     /locus_tag="VF240_05650"
     CDS             complement(8663..9469)
                     /locus_tag="VF240_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX7174748.1"
                     /translation="MPERAIEEYTVTDEPFYLAVGGEVELFEAAHAARLPVMLKGPTG
                     CGKTRFVEHMAWRLRRPLITVACHEDLSATDLVGRFLLEGDETVWHDGPLTSAVRHGA
                     ICYLDEVVEARKDTVVIIHPLTDDRRRLPIEKRGTILDAPPDFMLVVSYNPGYQSILK
                     DLKQSTRQRFVALEFDYPPPEPEAEIVAREGGVDLQTARDLVLIGQKVRNLRGHGLEE
                     GVSTRLLIYAAQLTARGIPAVTAAEVALCSPITDDRDLQRSIREIVTTVI"
     gene            complement(9517..9906)
                     /locus_tag="VF240_05655"
     CDS             complement(9517..9906)
                     /locus_tag="VF240_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174749.1"
                     /translation="MSSEERILRLENAMATLAELSADHENTMATLSQLSADNQRLLAD
                     NQRRTARLEESLVTLVELTRSHNEGMDELRAAQVESDRKISALADAQLHTDEALKQLA
                     EAQAHTDRRLDALIDIVRDGRNGRSEG"
     gene            10073..>10317
                     /locus_tag="VF240_05660"
     CDS             10073..>10317
                     /locus_tag="VF240_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX7174750.1"
                     /translation="MKIAVAMSGGVDSSAAAAILKAQGHELVGFTMQLWNQRRGLSVD
                     ADGEPLPSRCCSLDDVYDARRVAAELGFPFYVLNLER"
BASE COUNT         1714 a         3315 c         3363 g         1925 t
ORIGIN      
        1 ctgtagaatc cgaaacaggg caaacgcttt tacgcgcccg tcgtcgtcac gcctctacgt
       61 ccccatgaat catctggatg aactgaaaga atttgtgacg gaaggccgca gcgtccggcc
      121 ggtcgaggag ggcatctaca cggtgctcgc cgaaggagcg cgcgccgcgc acctctacga
      181 ccggcgcgcc ggcgtctacg acgcgctcgt gggcacgcgg ctctacaacc gcgtgatgtg
      241 gggggcttcg ccggaagact acgccgcgtt cgcgcgacgc gcggtggacg cccgcgcggc
      301 cgggtggatg ctcgacgccg cgtgcggctc gatgctcttc acggcgcgcg cctacgtcga
      361 gacggggcgg cgcctgatcg cgttcgacca gtcgctgcgg atgctgcggc gtgcgcgtga
      421 gcgcctgcgc ggcttcggcg gcggtcgccc gtgcgcgaat gtccttctgc ttcaggcgga
      481 cttgagcgac ccgccgtttc gcccggcgag tttccagacc gtcctctgca tgaatgttct
      541 gcaccagttc gccgacgccg acggtctgat accgaagctc gccgggctgc ttgccgacgg
      601 cggccaacta catctgacga gcctcgtctc caaccggcgt ttcatcggcg accgctacct
      661 cgacgcactg caccgcgcgg gcgagttcgt ccggccccgc cgaagctccg agtttgagaa
      721 acttttgcgc gacgctctga gtcagggcgt cagctacacc acgcgcggca acatggcgtt
      781 cgccacgtgg gagcgcccga cgcccgcctg agattagatg acgacgaatc acatggacgc
      841 cgcgcgccac cccggccacg gcgcggaggt cgagcggctc taccgggaca tcgccgtgga
      901 gacgcgctac ggccactacg tccgcatccc cgaacgcatc tgccgctgtc tcgactactt
      961 cggagtccgc cacgagcgcg cggccgtcgc cgagcgtctg cactcctact atctcttcat
     1021 cggcgtggtg gacgacgcca tcgacgcgag ccgcgcgggc gccggcgagt cagtcctcaa
     1081 gcgtctggag gcgtcggcgc cgtcgttcga cgaggagacg cggcgcacgc gcacggggtt
     1141 gctgaccgag gtcttaaaga ctcacatcgg ccccgaagtt tacacgccgg ctcgacggaa
     1201 gctcgaagag ttgtacggcg cggtcgtcgg ggagcgcggg gccgggacgc tgggggcata
     1261 catcgagcgg cggaaaacgg tgggccggct caccacggag gtgtcctacc tgctcatcgc
     1321 cccgctgctc gaaggcggcc gcgacggtct gctcggcttc ctgcgacgcg tcggcgaggt
     1381 cggctgtctc gttgacagcg tcgtcgatct gcgcgccgac gagcgggcgg gcctcctcgg
     1441 cttccggccc acggcgcgcg accgcctccg gctcgccggc ctgaccgttt acgaagggct
     1501 gagaatgtgg tcggagcacc cgcgcctcac ccgcctgttt cttgaagcgg tcggcgacgt
     1561 gctgctcgac cgcctgcgcg cgcgccggcc gcagccggcg gcggacgggc gcgctcagtt
     1621 ggaagtcgag tattagaacg cgcttcaaaa ctgtaggggc agggcaagtc cgcggcccgc
     1681 actgcgaaaa gaattaacat ggatgggcag gatgggcagg atgggcagga taatagattt
     1741 gaaatctcaa atctgaaatc ttaaatcagc tctttcttca tcctcttaat cctgcccatc
     1801 catgttaatt ctttcggccg agcgggccgc ggacttgccc tgcccctacg gacatgatgg
     1861 gcgttgaggt cttggagcgg actcttagcc ggcgccagtt tgatagatag acgcgcggcg
     1921 gccccgtcac ttcttcgcga gcagcttgcg cgaggccaga aactcgccga gcgcgcggtt
     1981 gaactcatcg ggcttgtcca tcatcagaaa atggctcacg ccgtcccaca tgcggtagtc
     2041 gagctcgggt gcgatgcgtc ggaagaactg ctcattgtct gccggccaga agggcgagcg
     2101 cgcgagcacg gccagcatcg gcgccttgat ctggtcttcc ttccagactg actgctccat
     2161 catcccgtcc atcgcgctga cggcgacgtg ctgcggcgtc gccagcatcg ccgccctgat
     2221 ctccgcacgc agcgcctctg acttcaccgg ctgcaacatc ccgtccacca tgcgcgccgc
     2281 attctccttg tagtcggagc cgcgcagggg ggcgatgaac tgctccatcc gctcgcgcgg
     2341 ggcgaagggc cgaagcccgc cgtccacgtt gacgagcgcg agggtctttt cggggtagcg
     2401 ccggtagaac tgtcggacga cgggcatgcc catgctatgc ccgacgagca cggcgcgctt
     2461 cacccccgcg tcgcgcatca cggcctcgac ggcgcgcgcg aacaggtcca tcgtgtaggc
     2521 cacctgctgc ggcttgtcgc tctggccgtg gcccggcaga tcgacggcga ggacgcgcga
     2581 cttcgcggcg aacaccggcg tctgcatccg ccagaagtcc agattgcacg tccacccgtg
     2641 tacgaacacc agcgcctcgc tccccttccc cgcgtccgcg taatgcacgc gcatgttctc
     2701 gaatctggcg aagcgcgagc gtcccacgcg cgccccctcg cccgcgccct ccgcgcgcgg
     2761 cgcgtgcgaa aaggcggcgc acacgacgac agcgagggcg cagaagatgg cgcgtcgggt
     2821 tgaaagattt gtagacatca tgtcgctcct ctcttgggtg ttgtgtggag atagtacgtc
     2881 gtcaatgatc gtgtaactga cttcgccgta tggagcatgc acctggctct ctaccggaat
     2941 gggaggaatc tgatctgacc ttcccattca ccctcaaggc tgcattcggc aaggagaagt
     3001 atgtcttcaa tggcctgggc gatgggggtg tgctgactaa tttcaaagac tccgagcata
     3061 ggcaatcctg cggcgatgcg ctcatcgaca tattgcttca tcgtcgttac atcatgtgtg
     3121 agcaaaacgc ggctttccgt cgcggcccat tcgagcactt ccgcatcccc tttgcccagc
     3181 agtccggcat cctgaacacg cacgatgtcc agatcacttc tgcggcgcag caacccacgc
     3241 aagatacgat tattgaagtc ttcgtcagtc gctaggcgca gcattaactt ctatccttcg
     3301 tcggctcgaa cgagtgacct cacccttcgt caaaggggcg ctctttccaa ctgagcatat
     3361 cctatcggtc aagcgacttt tttcagaagt tctcataata aaataggttc tggaacaagt
     3421 atttaaaaca aaggcccgcc atttccagcg ggcgagtaaa gggtctcaca ctataatttg
     3481 tagttgtaag gatggaatgc tatcaccgca cagtttattg cttgcgggcc aaaaggcgct
     3541 cgcgcaatcc gtgagggttg aagcgcgctt caagctcttt tttcagttcc tgacgttcta
     3601 cttttctttg ctccaggtac tgttctattt cggcgcgatt ctgtaggtag tagccgagaa
     3661 cggaatagat ggcagctaga tcaagcgtcg gaaaggcttg ggcgatttct tccggcgttg
     3721 acccttcgtc aaaggcgtaa atgactgagt caagcgacac acgcgtgccg gcgacgcgta
     3781 aaacgcctcc ttgatcctta gataacggca ctgtagttgt tccctgactc atcgctgaaa
     3841 gtatagctca agtagcccaa gcctaacatc gaaatccgta cttttatgtc gtcaaacgtc
     3901 tgtaaatcgc cggcatccgt tccggcagcg agagcacgtc gtcgatgatc gtgtaaccca
     3961 cttcgccgta gaggtcgcgg agttcggttt cggagtcgcg gtcgatggtg atgcagaagg
     4021 aggtcacgac gcggcgtcgg gcttgggtgt ttgacaggcg ccttctcgga caacagctca
     4081 ggccgcctca aaagaccggc tctcaggtgc gtccggttaa ctcgtcgaag gccagccaga
     4141 tttccttcag cgccgcgccg gagaccctgc cgaggcgggc gccgacgaat ttctgatcaa
     4201 ctgctgtgag ctgaaagacg agcgcgacgg agggctggcg cagcccgttg tgttcatccg
     4261 gctcgactag caccgttgcg gcaaaacgca gagcgtcctg ctgggaagtg agcggtatca
     4321 gcaacacggt cgggagcgcg gacgcttgct ggacgatgat ggcggggcgt cttccggtct
     4381 gagcatgtcc gccgcgagcc ggcattgcaa cccaacgcaa ctcgccgacg gtcatggctc
     4441 cgtctcctcg aaaggaaaca tctcccagct ttcgttgccg attgcctgcc actcccgcaa
     4501 ctcggaggcc agcgcctcac ccatcggctc atcggtcaga ggaatgagca ggatgccttt
     4561 ctgcgtttcg atgatgcgga cgatggtctc ctccgccaga ccgtaacggt cgagcacgtc
     4621 gcttgggagc gtgattcttc cgccgccctc aacgatcagt ttttcaatga cactcatggc
     4681 ttcctctcgc ttcaggaaaa ttcaaggcac agtcagtata acaacagttc gttcatggcg
     4741 cgcgccgctc acgtcgtcag ccggcggtaa atggcgggca tgcgctcggg cagcgagagc
     4801 acgtcgtcga tgatggtgta gccgacttcg ccgtagaggt cgcggagttc ggtttcggag
     4861 tcgcggtcga tggtgatgca gaagggggtg atgccgctcg cgcgggcatg gcggagggct
     4921 tcgcgggtgt cttcgcgggc gtagcgggcg tcgccgtagt cgtggtcgta ggggcgtccg
     4981 tcggagagga cgatgaggag gcgggtgcgg gcgtcgcgtt gttggaggcg cgcggcggcg
     5041 tggcggatgg cggcgccgag gcgggtgttg tgctggtagt tgatgccgcc gatgcggcgc
     5101 tcggtctcct ccgtgtaggt ctcgtcgaaa tccttgacga cgtaaaattt cacgttgcgg
     5161 cgtccctccg aggtgaagcc gtagatggag taagagtcgc cgacggcttc gagcgcctcg
     5221 ctcatcagga cgagtccctc cttctcgacc tcgatgatgc ggcggcccgg atgcgtgtag
     5281 ggctgcaagg ggtggcggcc gatggtgcgc gccgtcgagg aggattgatc gaggaggaag
     5341 gagacggcca cgtcgcgctc gcgccggagt cgtttggtgt agatgcgctc ggactgttgg
     5401 ccgtcgttgg cgcggcgatc catgacgtag tcgatgacgg cggagaggtc gtagtcgtcg
     5461 ccgtcaagct cgttcgggat gcgccgcagg ttctccggct tcatcaactg gaactggtgg
     5521 cggatcgagg agatgacgcc gcgatagcgc gtgcgcgtct gctcgacgaa ggcgcggtcg
     5581 ccgcggcgca ctttcttctc gatgacgcgg caccagccga cgcgcgtgtc ggagagttcc
     5641 ctgtcccact cgtcgtagct gtaggcgacc tcatccggct caagctcctg ttcggacgac
     5701 tcggccgatg tccaacgctc cgtgcccgtg aacgcctcct cgccgtcggc gtccgagacc
     5761 tcggaggcgg cccacgcgtt gaacagctcg cgcgcgtcgc ggcgacgggc ctcgtcgcgc
     5821 cgccgctcgc gctcctcctc gccctcttcc gtctcctgcg ggagacgatc ctgcgcgccc
     5881 gagctttcgg cgtcgcccga ctgctccggc gcgtcctgct gccgctccga catgtcgggg
     5941 acgaccgcct gaaagagcgt gtagacgcgc gaggtggcca tcaaggtgtc tccgacctcc
     6001 gcgtcgggac tcgtgaggta gtcgatgacg attgactcca gctcggagac gatctgtccg
     6061 taggaggcgc gcgcgtcgtc ggacgcgccg ccgcagagcg tgatctggaa gagcaactcg
     6121 aaggggacga gcgcgatggg aagaccggcg acacgcgggc ggttctcgcg caggtgcgcg
     6181 cggactaggt cgaggtcgcg cgccagaccg cggtaggcgt ggcgcagtcg gcggtcgatg
     6241 cgcccgttct ccagcgtgcc gaagatgcgc gcggccagcc ccttttgtgg gaagagggcg
     6301 agcgcggcgc ggtaatcggc ggcggcggga agctcgcgcc gtcgctcgcg ggcgaggcgc
     6361 gcctcctcct cgggcgggag tgcgcgctcg cccgccggga tgtcgttgat gtagccgtcg
     6421 agggcgaagg cgtcgagggc gtcggcgtgc tcgggcgcat agaggtcggc cagagaactg
     6481 taggcggcgc gcagggaggc ggagtcgcgc gcgtgcgtgc caaactcgat ctgcccggcg
     6541 gcgtgcgcgg cgagcacctt gtagagtcgg aagtcgcgcg cctcgtcgtc gaactccgcg
     6601 acgctcgacg gcaggtgaat cgtccggccg tcgccgatgc gcgactcctc cggcacggct
     6661 gagagcggcg cgacctcgac ggaacggccc gtcagcccct cgacgtagag ccggagcgtc
     6721 tgcgccacct cttcgagcgc gaggcccgcc ccaccgcccg tcagacgctc gttgctccgg
     6781 cgcgtctcca gcgcgtagta actgcgacgc gctcgcgcgc tcgtcgcccg ctcatccgtg
     6841 agaccttcgg ccgcccagtg ctcgaactgt tcgatggagg tcgagcgcag cgcccgcggc
     6901 cccgaacgga agcagcagat ggcggcctcg gcgtccacgc gcgccacctc gcgcgaagcg
     6961 cggacgacgc gccccgccag ctccgaggcg cgctctcgat tagtccgctc gacgacggcg
     7021 cggaagaacg cgggcgacgc gcgggccagc gcgacgagcg cggcgttcga ctgctcggcc
     7081 acctcgcgca acaggtcagt ccagtcggca aacgtctcgg gcagcgcgcg cagcacgccg
     7141 ccgccggcga cgaacacgtg cagggccgcc gcgccgccgc ggtcgaggaa gtcttcggcg
     7201 cagcggaaga gccggagcgc gtcccgttcg ccgagtccgc cgaccgagtc gggcaacgcc
     7261 gcccacatgt ccgacgcgat accgcccgcg cgcgtcgcga agacttcggc cagcctcacg
     7321 cccgcgcgct tcgtcgcggc tgccccgcct gcgcccccgc ccacggcgcc cagcgcctcg
     7381 acggcgcgcg gcgtggcttc gaggaattcc gcgctgtgct tcgccgagcg gcgcgcgatc
     7441 tcgaccgcca cctcgtagac cgagcgcaag aggcccgcgt cgccgaccgc gcgggccagc
     7501 cctgccgcgc cgcgaaaagt ttcgagcgcg attgaggtcg agagcgtgga ctgtcgcgcg
     7561 cacaccagaa acagtacggg cagcgcctcg cgcggcacgc cctcaaggtc tgagacgccg
     7621 gccgcgaaga actccgcgcc cgtgtcgagg tcggccatcg ctaaacgccg gcccatctcg
     7681 ccccacgcgc gcagctcctc gtcggcgagc acctgcgccg catcaggcgc ggcccgcaaa
     7741 aattccacgg cgacgcgcag gctcaacccc gccagccccg cgcccacctc cagcgccgcg
     7801 cgcgcgctct ccactggcag attgccgatc tcccgcacca gccccagaat cgtccgccgc
     7861 tccccgaaac tccgcaccga gcgcagacgc tcgcgcaacg ccttatcaag cggctccgcg
     7921 ccctccggcc tttcgtcccg cgctttcgtg ttgagtccgt ggtcttgcgt cttcattcaa
     7981 tttctctgac gaacgatctc ggaaacatta gtccgcgcgt caacgtggat atcgctttgc
     8041 gcgaattgta cagcaagtgg tcggggggca tccaaacagg ccgttacagt tgagccagga
     8101 gcttatcgta gtcagcgtgt gagccgatcc agtaccagac catcgtgtcg ccttctttaa
     8161 ctccgagcgc acgccagccg atgccgatac gcactgaata tattggctcg cgggtgtgca
     8221 cctgcttgaa ttgaagactt ccgtggtgtg ggtctttttt ccagaggcgg tatgcttttc
     8281 cggcctgacg ctgaatgtgc ggcgggagtt ggcggagggc tttgcggaat ctttcggtcg
     8341 tgtgtgaggt cacaggtctt cgtcgagggg cttggtttcg ccccgcccga actctgccag
     8401 cgcctcatcg gcgagtgccg ccagcttgtc ctgcgagtta gcaagggcgc cgtcccaaag
     8461 ctcctccgcg gcgaggtctt tgagcagcat cgtagccagc gcgttctgat cttcctcaga
     8521 aagcttggac gccgcagtga atgctttttc gagtaactct gtcatctctt caacgcgccc
     8581 cggtgatggt tcaaagtcaa atgtagcgta gttcagttta ccgcgccaac ccggcaatca
     8641 cagggccgtt ttgccctcga ctctaaatca ccgtcgtcac gatctcgcgg atgctgcgct
     8701 ggaggtcgcg gtcgtcggtg atgggcgagc agagggcgac ttcggcggcc gtcacggcgg
     8761 ggatgccgcg ggcggtgagc tgcgcggcgt agatgaggag gcgcgtggag acgccctctt
     8821 cgaggccgtg gccgcgcagg tttctgacct tctgaccgat gaggacgagg tcgcgcgccg
     8881 tctgcaaatc caccccgccc tctcgcgcga cgatctcggc ctccggctcg ggcggcgggt
     8941 agtcgaactc cagcgcgacg aagcgttggc gggtggactg cttgaggtct ttgaggatgg
     9001 actgatagcc ggggttgtag ctgacgacga gcatgaagtc agggggcgcg tcgaggatgg
     9061 tgccgcgctt ctcgatgggc aggcggcggc ggtcgtccgt gagcgggtgg atgatgacga
     9121 cggtgtcctt gcgtgcctcg accacctcat cgagataaca gatggcgccg tggcggacgg
     9181 ccgaggtgag cggcccgtcg tgccagaccg tttcgtcgcc ttcgaggaga aagcgcccga
     9241 cgaggtcggt ggccgagagg tcttcgtggc aggcgaccgt gatgagcggc cggcgcagac
     9301 gccacgccat gtgctcgacg aagcgcgtct tgccgcagcc cgtcgggcct ttgagcatca
     9361 cgggcagacg cgcggcgtgc gccgcctcaa atagctcgac ctcgccgccg accgcgaggt
     9421 aaaacggctc gtccgtaact gtgtactctt cgattgctct ttcgggcatt ttactattat
     9481 cgaaattcag tcgtttcgag tttgccgacc gccggcttag ccttccgacc gtccgttgcg
     9541 tccatctctc acaatatcaa tcaacgcatc cagccgccga tccgtatggg cctgggcttc
     9601 cgctaattgc ttcagcgcct catcagtgtg aagttgagcg tcggccaagg cagaaatttt
     9661 tctatccgac tctacctgcg ccgcgcgcaa ttcgtccatc ccttcgttgt gactccgggt
     9721 taactcgacg agcgtcacca atgactcttc caagcgtgcg gtgcgccgct ggttgtcggc
     9781 caacagccgc tggttgtcgg ccgacagttg tgacagcgtg gccattgtgt tttcgtgatc
     9841 tgccgacaat tcagccagcg tggccattgc gttttcgagg cgaagtattc tctcttcgga
     9901 cgacatttcg cacctccggc agcatttcgc ggcctccgct gtgctggcgt ttcctcgcgc
     9961 atggggtatc ctgtgcgtgg gtagaaaagg cagccggagc cgtaaggcag tcatcttaac
    10021 atcgcctcaa acacgcggca aatttgggct tttggagtga agttaatctg agatgaagat
    10081 tgcggtggcg atgagcggcg gcgtcgattc gtcggcggcg gcggcgattt tgaaggcgca
    10141 ggggcacgag ctcgtcggct tcacgatgca gctctggaac cagcgccggg gcctcagtgt
    10201 ggacgccgac ggcgagccgc tgccctcgcg ctgctgctcg ctcgacgacg tgtacgacgc
    10261 gcgccgcgtc gccgccgaac tcggcttccc cttctacgtc ctcaacctcg aacgcga
//
LOCUS       DASVES010000147         8630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_376805, whole genome shotgun sequence.
ACCESSION   DASVES010000147 DASVES010000000
VERSION     DASVES010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376805"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF240_05665"
     CDS             <1..193
                     /locus_tag="VF240_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7174751.1"
                     /translation="VYDVTGAGDTVIATLAAALSAGATLVEAAMLANHAAGIVVGKLG
                     TATATADELLSSIKSDGQV"
     gene            241..891
                     /locus_tag="VF240_05670"
     CDS             241..891
                     /locus_tag="VF240_05670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7174752.1"
                     /translation="MSFALCVALAAVLGACQACGGEPAQATPVVTPTSQAGAVAVNGE
                     RGATQADEALNSKPLKLPLSRPKVVVEKSARRLSLYSGGEVVRSFRIGLGEKPEGDKV
                     REGDRRTPEGTYYVCVKNAQSAFYLSLGINYPNAEDAERGLRDGLITRAQYRKIVRAA
                     KRNATPPWNTTLGGEIFIHGGGSQSDWTWGCVALENAHVKELFDSLPLGTPVHIEP"
     gene            918..2681
                     /gene="dacB"
                     /locus_tag="VF240_05675"
     CDS             918..2681
                     /gene="dacB"
                     /locus_tag="VF240_05675"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX7174753.1"
                     /translation="MPLQKLLSTRLSTLLLTFALLSGASLPAASAQATQRARRAVTAP
                     PATPTPSPTPSRNAPAPASVTPTAEQKPTPLPEASPTPTPTTAPASVRAAPRTLEELR
                     ARIRELSQQPALASSRLAVKVASLETGRVLYEEEANKWMQPASNMKLYTVAAALDRLG
                     PDYRFATSVYAPARPDLAGAVRGDLTIYGRGDPTYATRFNTPAAPAGTTGAPVAAVQP
                     ADYYKAIDELAARVVASGVRRVEGDLVGDESYFTGGALPAGWEWDDLQWYYGAEVSAL
                     TINDNAVDLNIRPGAREGDPLRISVGPATQLVTVIDRTRTAARGTRRDLTVRRALDED
                     VIEVSGTMPLDDRPYTGAVAVTRPALLFASMLRESLERQGVQVTGRTRTMDARARAAS
                     PLQVSSLVELAVRQSPPLSLIAAQTMKPSQNLYTELTLRALGKATATEAKLAEDEAGI
                     KAVKDFIERAGVGRDKLVMLDGSGLSRANLVTADSTLQLLIHMSRHRHAAVFREALPV
                     AGIDGTLRNRMKTTLAVGNVRAKTGTLGTATTLSGYVLSAGGEQLAFSIMVNNPPREA
                     DARSLFTEPVAVLLASFTGHS"
     gene            2805..3722
                     /gene="dapF"
                     /locus_tag="VF240_05680"
     CDS             2805..3722
                     /gene="dapF"
                     /locus_tag="VF240_05680"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7174754.1"
                     /translation="MRFAKFHGYGNDYIVFEARELSAAAGWSNDFVRIGEVGPFGDFV
                     RRVCHRHYGAGADGVAVVEPADSGSVADFRLRIFNADGTEAAMSGNGSRCAAAYLHHL
                     KLWTADELRLTTRAGVKRYTLRGRPGPGRYLFEAEIGRPLFDPASIPFAAPAAAGPLP
                     EVRDFPLSLPTGETVAVTALQMCNPNCCVFVDDFDAADWRRTGSLIEPHPQFPEKTNV
                     EFVRVLGPGEIEVRVWERGVGETLSSGTGASAAAVASAHTGRTGRRVRVHMPGGALEV
                     FWRDDGEVLLTGTAEAVYSGEWLKGRDEG"
     gene            4105..4710
                     /locus_tag="VF240_05685"
     CDS             4105..4710
                     /locus_tag="VF240_05685"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX7174755.1"
                     /translation="MSKSLLGGGAATAVALLSASMLFYTQTSIAEPLASTIQGQQTQQ
                     KTAAHSLREESTPAADPAEAPNAVASEMVSGIAGPAAKTPERLTGTTAAVASPAQAYT
                     ATAYSLRGRTASGRGVSRGLIAADRRVLPLGTRVRLDAGSYSGEYLVADTGGLVRGRK
                     IDIWMPSTSEAMRFGRRTVKLTVLTYGGRRSTPQPRSLIRK"
     gene            4868..5905
                     /locus_tag="VF240_05690"
     CDS             4868..5905
                     /locus_tag="VF240_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX7174756.1"
                     /translation="MPRAAPLVEVWRGSLVESRHRGHAAAVDGEGRLVASLGEPETVT
                     YMRSSAKMHQAIPLIVSGAAERFEFTDREIAIVCGSHSGQDIHAETVAGMLRKTGLDE
                     SALRCGTHEPFDQQTARELRERHETPGVLRNNCSGKHAGMLALATHLGAPVESYERPE
                     HPVQRAMLETVSRFSGVAAGEIPLGTDGCGVPVFGLSVRSMAHMFARLVAPPRDFDAK
                     TGDACARIVRAATTHPEMVGGTRERFDTGVMEAARGRLVSKVGAEGVYAAGVLPCDEW
                     PRGLGLAFKIDDGEDRRARPTVAVEILRQLGVLVGEALDAAAPYASFPIRNHRGDTVG
                     IVRAVLTLERT"
     gene            complement(6440..7606)
                     /locus_tag="VF240_05695"
     CDS             complement(6440..7606)
                     /locus_tag="VF240_05695"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEX7174757.1"
                     /translation="MFRTLIRFVLIASSLAVLFIPTFAQAVSVKEAAPLLPGRVGDLR
                     AQGPVEEDDFRLSGNPEDFRILARARRLYVSPDGETFKVTLIKTSTDSAAFSLLTHLA
                     PNHPTSPIKSGDVGTASISSSGGVFFFKGPAAVEVSPATESANNDALLLNVARLIAEG
                     LDAGEGGVPVLALHLPEWEKVQGQVDYAVSLPVLKAQTGNEPVLDAVSFEGGAEAATA
                     PYGDGRLVVVEFTTPQYATDNDARVTQRIDELRASAERVPAAYRRVGNYAVFVFGVPD
                     AQRAATLIDQVKYEKDVRWLGRNPHEQTMRERYLTTTMGGVVVTTLKATGVAILVCLG
                     VGGLFGGVIFLRRRARSAASEIFTDAGGMLRLDLEDVNSRRVRPGGLLDSGVKK"
     gene            complement(7737..8438)
                     /locus_tag="VF240_05700"
     CDS             complement(7737..8438)
                     /locus_tag="VF240_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7174758.1"
                     /translation="MAIRQFCMNCGEQLAPAARFCPTCGATADSEVTRLASEETRVSS
                     PARTPLGPPTRPPQPVVPIPRPHEPSAAAPRTQTGRQEDDAERVVFTVRPTMLFIKIG
                     YLLAALGGILLVALFAYFAPDPRLTYVSFILALALLLIPAYHHVRRQMVRYTLTDSKI
                     EIDEGFISQTTRNLPLRNIQDVTVKTTVFQRLCGFGDLVIDNANETGGPTVLDNIPEP
                     RRHMDLLLRELRRWR"
BASE COUNT         1505 a         2880 c         2836 g         1409 t
ORIGIN      
        1 ggtctacgac gtgacgggcg cgggcgacac cgtcatcgcc acgctcgccg ccgccttgtc
       61 ggccggggcc acactggtcg aggcggcgat gctcgccaac cacgccgccg gcatcgtcgt
      121 cggcaagctc ggcacggcca cggcgacggc cgacgagctg ctgtcttcga ttaaatccga
      181 cgggcaggtt tgagcatcaa acgctttaag ctgtcgatga agagaccgtg gagatcaagt
      241 ttgtcgttcg ccctgtgcgt cgccctcgcg gcggtgcttg gtgcttgtca ggcgtgcggg
      301 ggtgagccgg cacaagcgac gcccgttgta acgccgacat cccaggccgg cgcggtggcg
      361 gtcaacggcg agcgcggcgc gacgcaggcg gacgaagcct taaacagtaa gccgctcaag
      421 ctcccgctgt cgcggccgaa ggtcgtggtc gagaagtccg cgcgccgtct gtcgctctac
      481 tcgggcggcg aggtcgtgcg ctcgttccgc atcgggctgg gcgaaaagcc cgagggcgac
      541 aaggtgcgcg agggcgaccg gcgcacgccg gagggcactt actacgtctg cgtgaagaac
      601 gcgcagagcg ccttctatct ctcgctcggc atcaactacc cgaacgcgga ggacgccgag
      661 cgcggcctgc gcgatgggct aatcacccgc gcgcagtatc gaaagatcgt gcgcgccgcg
      721 aagaggaacg cgacgccgcc ctggaacacg accctcggcg gcgagatttt catccacggc
      781 ggcggctcgc agagcgactg gacgtggggc tgcgtcgccc tcgaaaacgc acacgtcaaa
      841 gagttgttcg actcgctgcc gctcggcact cccgtccaca tcgaaccatg acaagcaagg
      901 gaaacgtcat ccgccatatg ccacttcaaa agcttctctc gacacggctc tcaactctcc
      961 tgctgacctt cgcgctcctc tcgggcgcgt ccctgcccgc cgcctcggca caggccactc
     1021 agcgcgcccg ccgcgccgtc accgcgccgc ccgcgacgcc gacgccctcg ccgacgcctt
     1081 cgcgcaacgc gcccgcgccc gcctccgtca ccccgacggc ggagcaaaag ccgacgccgc
     1141 tgcccgaagc gtcgccgacg cccaccccga cgactgcgcc cgcgagcgtc cgcgccgcgc
     1201 cgcgcaccct cgaagagctg cgcgcgcgca tccgggagct ttcgcagcag cccgcgctgg
     1261 cctcttcgcg cctcgccgtc aaggtcgcct cgctggagac ggggcgcgtg ctctatgagg
     1321 aggaggcgaa caagtggatg cagccggcct cgaacatgaa gctctacacg gtcgccgccg
     1381 cgctcgaccg cctcggcccc gactaccgct tcgcaacttc cgtctacgcc cccgcgcgcc
     1441 ccgacctcgc gggcgccgtc cgcggcgacc tcaccatcta cggccgcggc gacccgacct
     1501 acgccacgcg cttcaacacg cccgccgcgc ccgccggcac gacgggtgcg cccgtcgccg
     1561 ccgtgcaacc ggccgactac tacaaagcga ttgacgagct ggccgcgcgc gtcgtcgcct
     1621 cgggcgtccg tcgcgtcgag ggcgatctgg tcggcgacga gagttatttc acgggcggcg
     1681 cgctcccggc cgggtgggag tgggacgacc ttcagtggta ctacggggcc gaggtgagcg
     1741 cgctcactat taacgacaac gcggtcgatc tgaacatccg accgggcgcg cgcgagggcg
     1801 acccgctccg catctccgtc ggccccgcca cacagctcgt caccgtcatc gaccgcacgc
     1861 gcactgccgc gcgcggcacg cgccgcgacc tcaccgtgcg ccgcgcgctc gacgaagacg
     1921 tcatcgaggt ctcgggcacg atgcctctgg acgaccgccc gtacacgggg gccgtcgccg
     1981 tcacgcgccc ggcgctgctg ttcgcgtcga tgctgcgcga gtcgctggag cggcagggcg
     2041 tgcaggtcac ggggcgcacg cggacgatgg acgcgcgcgc gcgcgcggct tctccgcttc
     2101 aggtctcttc gctcgtcgag ctggccgtca ggcagtcgcc gccgctctcc ctcatcgccg
     2161 cgcagacgat gaagcccagc cagaacctct acaccgagtt gaccctgcgc gcgctcggca
     2221 aggccacggc caccgaagcg aagctggcgg aggacgaggc cggcatcaag gccgtcaaag
     2281 acttcatcga gcgcgcgggc gtaggccgcg acaagctcgt gatgcttgac ggctccggcc
     2341 tctctcgcgc caatctcgtc acggccgact cgacgctcca actgctcatt cacatgagcc
     2401 ggcaccgtca cgccgccgtc ttccgcgagg cgctgcccgt cgccggcatc gacggcacgc
     2461 tccgcaaccg catgaaaacg acgttggccg ttggcaacgt gcgcgctaag acgggcacgc
     2521 tggggacggc caccacgctg tcgggctacg tcctgagcgc gggcggcgag cagctcgcgt
     2581 tttccatcat ggtcaacaac cccccgcgcg aggcggacgc gcgctctctc ttcaccgagc
     2641 cggtcgccgt cctgctcgcc tccttcaccg gccactcatg agagttcaaa gttcaaagtt
     2701 caaagttcaa agcgcgcggg ggcggccgtt gactttcaac tctgaacctt gaactatgat
     2761 ttaaactttg aactttgaac tttgaacttt gaactcctga gttgatgcgc ttcgccaaat
     2821 tccacggcta cggcaacgac tacatcgtct tcgaggcgag ggaactatca gcggcggccg
     2881 gctggtcgaa cgacttcgtg cggatcggcg aggtcggccc cttcggcgac ttcgtgcgcc
     2941 gcgtctgcca ccggcactac ggggcggggg ccgacggcgt cgctgtcgtc gaaccggccg
     3001 acagtggcag cgtggccgac ttccgcctgc gaatcttcaa cgccgacggc acagaggccg
     3061 ccatgtccgg caacggctcc cgctgcgccg ccgcctacct ccatcacctg aagctctgga
     3121 cggccgacga gctgcgcctg accacccgcg ccggcgtcaa acgctacacc ttgcgcggac
     3181 gccccggtcc cggccgctac ctcttcgagg ccgaaatcgg ccgccccctc ttcgaccctg
     3241 cctccatccc cttcgccgcc cccgccgccg cagggccgct ccccgaggtg cgcgatttcc
     3301 cgctctccct acccaccggc gagacggtcg ccgtcacggc cctccagatg tgcaatccca
     3361 actgctgcgt cttcgtcgat gacttcgacg cggcggactg gcggcgcacg gggagcctca
     3421 tcgagccgca tccgcaattc cccgagaaga ccaacgtgga gttcgtccgc gtcctcggcc
     3481 ccggcgagat cgaggtgcgc gtctgggagc gcggcgtcgg cgagacactt tcctcgggga
     3541 cgggcgcctc ggccgcggcc gtcgcctcgg cccacacggg ccgcacgggc cggcgcgtcc
     3601 gcgtccacat gcccggcggc gccctcgaag tcttctggcg cgacgacggc gaggtgctcc
     3661 tcacgggcac cgccgaggcc gtctacagcg gggagtggtt gaagggaagg gatgagggat
     3721 gagggcggag ggatgaatga agtaagcagt gagcagtgag cagtaagcag tcaaggccgg
     3781 tcgagcgttc tgactgctca ctgctcactg cttactgctt actccctttc atccctcatc
     3841 cttccgccct catcccttcc tcatcggctg gactcctgtc acgaatttgt gttagccttt
     3901 tcacgaacaa gcgaacctcg caagggtgtt cgcttgtttc tcttttgtag accctccttt
     3961 cagcgcaacg catggggaga aggtttccca ctccggctga cgcctcgcac tccttgagcc
     4021 ggagaccgaa gggcgagacc cgcgaagggc cgcgcccccg acggggcact gaaagtttga
     4081 ctatcggaca ggagaaccga aacgatgagt aaatcccttc tgggtggagg cgccgccacg
     4141 gcggtcgcgc tcctctcagc ttccatgctc ttttacacgc agacatcaat cgcggaaccc
     4201 ctcgcttcga cgatccaagg gcagcagacg cagcaaaaaa ccgccgccca cagcttacgg
     4261 gaggaatcga ccccggcagc cgacccggcc gaggctccga acgcagtcgc ctcggaaatg
     4321 gtgtcaggga tagccggccc ggccgccaaa acgccggaga ggttgaccgg cacgaccgcc
     4381 gccgtcgcca gtcccgcgca agcctacacc gccacggcct acagcctccg cggccgtacc
     4441 gcgagcgggc ggggcgtctc ccgcggcctc atcgccgccg accgcagggt cttgccgctc
     4501 ggcacgcgag tccgtctcga cgccggctcc tacagcggcg agtatctggt ggcggacacg
     4561 ggcgggctgg tgcgcgggcg caagatcgac atctggatgc cgagcacgag tgaggccatg
     4621 cgcttcggcc gacggacggt gaagttgacc gtcctcacct acggcggaag acgctcaacg
     4681 ccgcagcccc gctctctgat tcgcaagtaa gacacgtttg acggggcacg tccgcgtcgc
     4741 ccggttcctg aggaggccgc tgaattcagc ggcctcttcg cttttccggg ggaggggatt
     4801 gatgttcgga cgccgacgcg gttaagatgc ctgccacttt cacatgagca caaacggaga
     4861 aacgcgtgtg ccccgtgcgg cgccgctcgt cgaggtctgg aggggttcgc tcgtggagtc
     4921 gcgtcaccgc ggccacgcgg cggccgtgga cggcgagggg cgtctggtgg cctctctcgg
     4981 agagccggag acggtcacgt acatgcgctc ctccgcgaag atgcatcagg cgattccgct
     5041 catcgtctcg ggcgcggccg aacgctttga gttcaccgac cgggagatcg ccatcgtctg
     5101 cggctcgcac agcggccagg acatccacgc cgaaacggtc gccgggatgc tccgcaagac
     5161 gggtctcgac gagtcggccc tccgttgcgg cacgcatgag cctttcgacc agcagaccgc
     5221 gcgcgagctg cgcgagcggc atgagacgcc cggcgtcctg cggaacaact gttcgggcaa
     5281 gcacgccggc atgctcgcgc tcgccacaca cctcggcgcc cccgtcgaat cctacgagcg
     5341 ccccgaacac cctgtacagc gggccatgct cgaaacggtc tcgcgtttct cgggcgtcgc
     5401 ggccggggag attccgctcg gcacggacgg ctgcggcgtc cccgtcttcg gcctgagcgt
     5461 gcgctcgatg gcgcacatgt tcgcgcgcct cgtcgccccg ccccgcgact tcgacgcgaa
     5521 gacgggcgac gcctgcgccc gaatcgtccg cgccgcgacg acccacccgg agatggtcgg
     5581 cgggacgcgc gagcgcttcg acacgggcgt catggaggcc gcgcggggcc ggctcgtctc
     5641 gaaggtgggg gccgagggcg tctacgcggc gggcgtcctg ccgtgcgacg agtggccgcg
     5701 tgggctggga ctggccttca agatcgatga cggcgaagac cggcgcgcac gccccacggt
     5761 cgcggtggaa atcctgcgac aactcggcgt cctcgtcggc gaggcgctcg acgcggccgc
     5821 gccctacgcg agcttcccca tccgcaacca ccgaggcgac accgtcggca tagtccgagc
     5881 ggttttgacg ttggagcgga catgagtttt taattcttac gggtaaatga attccggcgt
     5941 accgcaataa ttcaagcgta cgattggatt agtaccagag tcgtacaatt tgaattaact
     6001 gacgaggatg agcgaataaa gtcgcgttct gtgttgagag cggaaaattg ccggttttcc
     6061 cgcgaaatta aacgggtctg ggctggaatc gaagccatgc gggtctggtg gcacgctcgt
     6121 tgcaaaacga gtaatcgtta aatgtaaggg ggataacaat agagttatcg ggtcaggctc
     6181 ggagacggca gcgagtgaaa actttggggg aggttttctc tcactttaag gcaggcagcc
     6241 actctttcgg gggaacggag tagttgttaa gcctttagcg gtcaaagggc aaaagtcggg
     6301 ttgctggtcg acgttctgtg aaggctttgg gggaacctca cagataaggc cagcaacccg
     6361 caattttttt cgggtataca aaaagggcgc gctgactgcg gatagtccgc gcgccctttt
     6421 ttctacttcg tcggagcttt cacttcttca cgccgctgtc gagcagcccg ccgggtctga
     6481 cacgacgtga gttcacgtct tcaaggtcga ggcgcagcat cccgccggcg tccgtgaaga
     6541 tttccgaggc ggccgagcga gcgcggcgtc ggaggaagat gacgccgccg aaaagcccgc
     6601 ccacaccgag acagacgagg atggccaccc ccgtggcctt gagcgtcgtg acgacgacgc
     6661 cgcccatcgt cgtcgtgaga tacctctccc gcatcgtctg ctcgtgcgga ttgcgcccga
     6721 gccagcgcac gtccttctca tacttcacct gatctattaa agtcgccgcc cgctgcgcgt
     6781 caggcacgcc gaagacgaaa acggcgtagt tgccgacgcg ccggtaggcc gcagggacgc
     6841 gctccgctga cgcgcggagt tcgtcgatgc gctgcgtgac gcgcgcgtcg ttgtcggtcg
     6901 cgtactgcgg cgtggtgaac tcgacgacga cgagccgtcc gtcgccgtaa ggcgcggtcg
     6961 cggcctcggc cccaccctcg aaggagacgg cgtccagaac aggctcgttg cctgtctggg
     7021 ccttcagaac ggggagcgat acggcgtagt caacctgccc ctgaaccttc tcccattcgg
     7081 gaaggtggag agcgaggacg ggcacgccgc cttcacccgc gtcaagcccc tcggcgataa
     7141 gacgcgcgac attcagcagc agggcatcat tattcgccga ctccgtcgcg ggtgagactt
     7201 cgacggccgc cgggcctttg aagaaaaaga ctccgcctga acttgagata ctagccgtcc
     7261 cgacatcacc tgacttgatg ggtgatgtcg gatggttggg cgctaagtga gtcagcagcg
     7321 aaaaggcggc ggagtccgtg ctcgtcttga tgagagtgac tttgaacgtc tcaccgtcgg
     7381 gcgaaacgta aagccgccgg gcgcgggcga gaattcggaa gtcttcagga tttcctgaga
     7441 gtctgaagtc gtcttcctcg accggcccct gcgcgcgtag atcaccgacc cggccgggca
     7501 gcaacggggc ggcctcttta accgagaccg cttgggcgaa cgtcgggatg aaaaggacgg
     7561 cgagcgacga ggcgataaga acgaatctga tgagagttcg gaacacgacg aaacctttcc
     7621 cgtttaagaa ggcggcaatc agcacgacta gcttagacgc gcgccgggcc tgcggggttc
     7681 ccggaagagt cggattggcc gcttccttac tgattgattt tagggtttcg tcaatctcag
     7741 cgccagcggc ggagttcgcg caggagcaaa tccatgtggc ggcgcggctc ggggatgtta
     7801 tcgaggacgg tcgggccgcc ggtctcgttc gcgttgtcga tgacgaggtc gccgaagccg
     7861 cagaggcgct ggaagacggt cgtcttgacg gtcacgtcct gaatgttgcg cagcggcagg
     7921 ttgcgcgtcg tctgggagat gaacccctcg tcgatctcga tcttcgagtc ggtgagcgtg
     7981 tagcgcacca tctggcggcg gacgtggtgg taggcgggga taaggagcag ggcgagcgcc
     8041 aaaatgaagg agacgtaggt gagtcggggg tcaggggcga agtaggcgaa cagggcgacg
     8101 agaaggatgc cgccgagcgc ggccagcagg tagccgattt tgatgaagag catcgtcggg
     8161 cggacggtga agacgacgcg ctcggcgtcg tcctcctgcc ggcccgtctg cgtgcgcggg
     8221 gccgcggccg aaggctcgtg cggccggggt atggggacga caggctgcgg cggccgagtg
     8281 ggcgggccga gtggcgtcct cgcgggagag ctgacgcgcg tctcttcgga agcgagacgc
     8341 gtcacttcgg agtcggcggt ggcgccgcag gtcggacaga agcgcgcggc cggcgccaac
     8401 tgttcgccgc agttcatgca gaattgtcgt atcgccatga gccaagccgc gacatgtcgc
     8461 gggaagtcta actataacgc ggttgcagcc atcatacagg aagcaagcgg cgacaagaag
     8521 aggcaaggtg agggaaagaa tcgttcccga atcgtttcag cgattgtatc gaggtcacca
     8581 tcatcacccg atagatggct gaccggcgtg agcgggcagc catcaggctg
//
LOCUS       DASVES010000148         8433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1580068, whole genome shotgun sequence.
ACCESSION   DASVES010000148 DASVES010000000
VERSION     DASVES010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580068"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF240_05705"
     CDS             complement(<1..215)
                     /locus_tag="VF240_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174759.1"
                     /translation="MKLALILLCGVAALLARAPEAAAQKAATAAAPAVIKHGGKIEMK
                     YDGFNLETVVALRKMQVTCGSGKGFKD"
     gene            complement(614..1837)
                     /locus_tag="VF240_05710"
     CDS             complement(614..1837)
                     /locus_tag="VF240_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174760.1"
                     /translation="MAASTSDWPQWRGPARDGTSRESGLLKQWPKEGPKLLWQVNDIG
                     DGYSTPAVVGQRIYLMSNRGMENEFVQALSTQDGKPIWTTRVGNVGNPDQSPPYAKAR
                     STPTVDGSLIYALGSDGDLACLETKSGRVRWQKNIRTEFGGQPGTWAYAESPLVDGDV
                     VVVTPGGAQATMAALNKKTGAVIWKSAIPGGDPAGYASAIAVQGGGRKQYVQLLSKGL
                     VGVDAKTGQFLWRYAEAAKGPAQYFTPVGRNEYVYGGALGVGGGLLRLKPDGSGVVAE
                     QVYFTRGLPNGIGGAVLVGDTLYGTEAGQKLVAAEFTTGKVKWQADSFGWASAASADG
                     LLFLHLVTGEMVLVEATPEGFREKGRFTPPAQPKKKQVGQFPEMAFAYPVVANGRLYI
                     RDLGTLWAYDIKAKR"
     gene            complement(2384..3790)
                     /locus_tag="VF240_05715"
     CDS             complement(2384..3790)
                     /locus_tag="VF240_05715"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7174761.1"
                     /translation="MAKKGSILVVDDEEIMRDVLETLLSAEGYRVDLAKTGEEGLEVY
                     GRRPYDVVLLDVSMPGMGGLRTLEEILKTDSEGVVVMITAYATFDTAIGAWERGAFGC
                     IRKPFQNEQILSTVAEGIKRRRKEEERQTLRRAMSRSVERGSIIGRSDRMHAIFQLVD
                     QVAPARSTVLITGESGTGKELIAKAIHEQSPRIGRAFVTVNSSNIPSELLESELFGHT
                     RGAFTGAVAAKKGLFEVADGGSIFLDEIGDLPPETQARLLRVLQEREFTPLGDTTPRR
                     VDVRIIAATNVDLKEAVRQGMFREDLYYRLAVVPIELPPLRDRREDILLLAQHFIRKY
                     NEENARRVSDQLAPDVLALLEGYTWPGNVRELENVIERVVVIAPGDEVTRQCLPAEIA
                     DPQKSPGGAPLSGASDRIDLGRGVNFYDEVRRFEIDLIRRALEQTGGHQSRAARLLGM
                     NATTLNSKIKTYNIQLRS"
     gene            complement(4069..5115)
                     /locus_tag="VF240_05720"
     CDS             complement(4069..5115)
                     /locus_tag="VF240_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174762.1"
                     /translation="MTNVCGRRTTRARAPFGLLLVSVCFAASSSGGLSVGAAHSALGR
                     RPPHARPAPAQEGRVDIRLRSFTPTASGQLTIEPSAEGGRGRLTALGLPEAQTQSRDA
                     RAYVVWANSEGRIVRLGELGRDRRGNGGLAFNHPAGFERYTVIVTAEQSPDAERPLGA
                     PVLSTRAGEATAVFPVPAPPTVAASGPDPRPGGRNNVESLDPVGGSSRLRRTTGDFYA
                     EVDGALDARGGGRLIELEGASSAPRARGAARAAESAGRAYVVVRFRDVPLPQEAGAGV
                     YIMWAIQPSGRLVYMGSLPADEALNRADTYIRVAGFQSDDFDLFVTAEQRRPAPEPSD
                     RRVLSTRNARNTIR"
     gene            5276..6706
                     /locus_tag="VF240_05725"
     CDS             5276..6706
                     /locus_tag="VF240_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7174763.1"
                     /translation="MRRQGRALAVLLLCAMLFAQSSCRGDEGTSVSGRPAPHFKPGFN
                     LFSPEQDIELGEQSARQIAGEMPLLNDATTVNYVRALGGKLASHAPGHDFPYQFQVIG
                     AREINAFALPGGFIFVNAGAIAAARGEGEIAGVIAHEIAHVALRHGTNQASKAYIAQA
                     GLGVLGAIAGGGDSAEAGEIINAIGGAGANMLFLKFGRTAETQADLEGARIMAAAGYD
                     PRDMARFFETLAAEGGGGQRVPEMRSDHPDPGNRVAAINDILPQLRLGPTPTRDTQEF
                     QQVKARLTGGQLPRAAAPARTGPGDPNDIEPGARPPAPSANFRDYRSPDGYVRLTYPD
                     NWEALRLKGDEENLIFAPRGAYGEMDDAVYVTHGIFVGLVQPPARELEEANRVFVEGQ
                     IRANPDFRVRKGPQPVSFSNGSPGYATVVAGPSTVTGVVEIDVIYTTATRDGRLFYLI
                     TMAPEDEYERYHPAFEQIIRSVQLGQ"
     gene            complement(6825..7613)
                     /locus_tag="VF240_05730"
     CDS             complement(6825..7613)
                     /locus_tag="VF240_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7174764.1"
                     /translation="MKIPLYVIDAFTSRVFGGNPAAVCPLDAWLPDDVMQKIGQENNL
                     SETAFFVRESEDTYGLRWFTPTIEVDLCGHATLASAFAIFNFTDASADAVTFNSRSGP
                     LRVWRGDGERLTLDLPARRAKACDAPAALLDGLGARPREVLRSSVDYLAVYDTEDEVR
                     ALRPDMTLLAALDSLGVIVTAPGSASDCASRYFAPGAGIPEDPVTGAAHCTLTPYWAE
                     KFSRREIHALQVSARGGELFCEDAGERVKLSGHAVRYSEGFLYL"
     gene            complement(7702..8097)
                     /locus_tag="VF240_05735"
     CDS             complement(7702..8097)
                     /locus_tag="VF240_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174765.1"
                     /translation="MDTNENKSKWEGAGGTPGGMGEFLLGLGMAVAGAYLLASSVTVS
                     SGSWHVWGYNAFGLSLVPLIFGIGLLFYNGRSKIGWLLLFTGVIIIFAGIIMNLHIYF
                     QPTSLFGTIVMLVLLAGGVGLVARALRAH"
     gene            complement(8179..>8433)
                     /locus_tag="VF240_05740"
     CDS             complement(8179..>8433)
                     /locus_tag="VF240_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174766.1"
                     /translation="MIRQASYSYGMHGISLWILATDVKRFRPLVVFNAVAFLLAAPVF
                     FIIDYTSGMPWWWTVEDSLGCGLFGAALLWLSRGARKDEG"
BASE COUNT         1475 a         2709 c         2782 g         1467 t
ORIGIN      
        1 atgtccttga agcctttccc cgagccgcag gtgacctgca tcttcctgag cgcgacgacc
       61 gtctccaggt tgaagccgtc gtacttcatc tcgatcttgc cgccgtgctt gatgacggcg
      121 ggggctgctg cggtcgcggc cttctgggcc gcggcttcgg gagcgcgtgc gagcagcgcg
      181 gcgacgccgc acaggagtat gagcgcgagt ttcataaaac gacctcttgg taatcctggg
      241 attgccggcg gcgccggtcg gggtgaggcg aaaatttcgg agggcgaaag aggcggccgg
      301 tcaaacgcac tggctatcgc gcaccggccg ggccgcctga aaataaatat agggatgccc
      361 cggccggcgt gtcaacggta atgtcgcggg gcgttgtgag tccgcgccaa atcttatagc
      421 cgctttcagt tgagcgggta tgaagacaga ttcaccgcag agtcgcagag ggcacggagt
      481 tcacgcaaag aaggagtctc tgcgtgaact ccgtgccctc tgcgactctg cggtgagtct
      541 actccctgtt cactcaagtg aacgtcgctc taaactccca gcatctggga atcaacttaa
      601 tgtgcatcag tgcctaacgc ttcgccttga tgtcgtaggc ccacaatgtg ccgagatcgc
      661 ggatgtaaag cctcccgtta gcaactaccg gatacgcaaa cgccatctcg ggaaactgcc
      721 ccacctgttt cttcttcggc tgagccggcg gagtgaagcg gcccttttcg cgaaagcctt
      781 caggggtcgc ctccaccagc accatctctc cagtcacgag gtgaagaaat aaaagcccgt
      841 ccgccgaggc tgccgacgcc cagccgaaac tatcggcctg ccatttcacc tttcccgtcg
      901 tgaactcagc cgcgaccagc ttctgcccgg cttccgttcc gtaaagagtg tcaccgacca
      961 gcaccgctcc gccgatgccg ttcggaagtc cgcgcgtgaa gtaaacctgc tccgcgacga
     1021 ctccgctgcc gtccggcttc aagcggagga gtccgcctcc gacgccaagt gctccgccgt
     1081 acacatactc gttacgcccg accggtgtga aatactgcgc cggccccttg gccgcctccg
     1141 cgtagcgcca gaggaactgg cccgtcttgg catcgactcc gaccagcccc ttgctcaaaa
     1201 gctgcacgta ttgtttgcgc ccgccccctt gcacggcaat cgccgacgcg taacccgcgg
     1261 gatcgccacc cgggatcgcc gacttccaga tgaccgcgcc cgtctttttg ttgagcgccg
     1321 ccatcgtggc ctgcgcgccg ccgggggtga cgacaaccac gtcgccatca accagcggag
     1381 actccgcata ggcccatgtg cccggctggc ccccgaactc cgttcggatg ttcttctgcc
     1441 aacgaacacg gccgctcttg gtttcgagac aggcgaggtc gccgtcggag ccgagtgcgt
     1501 agatgagact gccgtcaacg gtcggagtgg accgggcctt cgcgtaaggc gggctttgat
     1561 ccgggttgcc gacgttgccg acgcgcgtcg tccagatagg ttttccgtcc tgagttgaga
     1621 gtgcctgaac gaactcattc tccatgccgc ggttgctcat caggtagatg cgctgcccga
     1681 cgaccgccgg tgttgaatag ccgtctccga tgtcgttcac ctgccagagc agtttcggtc
     1741 cttccttcgg ccattgttta agcaggccgc tctcgcgcga tgtcccgtcg cgcgccggcc
     1801 ctcgccactg aggccagtcg gaagtcgagg cagccatact ggcagacctc ggaacggaca
     1861 cggctaagag gcacaaacag agtgacgcaa aggtagtgag taatcgagac ttcattatct
     1921 tctccttaca gctatcgtaa atgcttttgg ggcttgctcc ccttcgcggg tacgcttcgg
     1981 tcgcccgcga cgcacgcttt aagcaggaag gtgttcttgc tccaaggcac gcagttctcc
     2041 ccgaagcgtc gcgctgcaaa cggttgttag aggccgctcg ccgagaacgc gacacctggc
     2101 gcttttcacc accttagtat tcgacgagcc gccttcgagg caatatcgtg cttccccctg
     2161 ccccgtaagc cgagagtgac gccgatggcc gagcgtccgt cttcggtccg acgaatggaa
     2221 ggcaacggct gtaaccttac ccagaccagt ctgtgctttg tctttcagct caggtactgc
     2281 tcgtgtcccg ccagttctac gagcgatgga gggaagatgt ttcttcgacg aagccgggca
     2341 agcccggatg cgtgatcacg gccgcaaccc tcgccggccg tgtttacgag cgcagttgga
     2401 tgttgtaggt cttgatcttg gagttgaggg tggtggcgtt catgccgagg aggcgtgcgg
     2461 cgcgcgactg gtgaccgccg gtttgttcga gggcgcggcg gatgaggtcg atctcgaagc
     2521 ggcgaacttc gtcgtagaag ttgacgccgc ggccgaggtc gatgcggtcg gaagcgccgg
     2581 agagcggggc gccgccgggc gacttctgcg ggtcggcgat ctcggccggc aagcactggc
     2641 gcgtcacctc gtcgcccggc gcgatgacga cgacgcgctc gatgacgttc tcaagctcgc
     2701 gcacgttgcc gggccacgtg tagccttcga ggagcgcgag cacgtcgggc gcgagctggt
     2761 cggagacgcg gcgcgcgttc tcctcgttgt acttgcgtat gaagtgctgc gcgagcagga
     2821 ggatgtcttc gcggcggtcg cgaaggggcg gcagctcgat ggggacgacg gcgagtcggt
     2881 agtagaggtc ttcgcggaac atgccctgcc gcacggcctc cttgagatcg acgttggtgg
     2941 cggcgatgat acgcacgtcc actcgtcgcg gcgtggtgtc gcctaagggc gtgaactcgc
     3001 gttcctggag cacgcgcagc aagcgcgcct gcgtctcggg cgggaggtcg ccgatctcgt
     3061 cgaggaagat ggagccgccg tcggcgactt caaacaaacc cttcttggcg gcgacggcgc
     3121 cggtgaacgc gccgcgcgtg tggccgaaca gctctgactc caaaagctcc gaagggatgt
     3181 tcgacgagtt gaccgtcacg aacgcgcggc cgatgcgcgg gctttgctcg tggatggctt
     3241 tggcgatgag ttccttgccc gtgcctgatt cgccggtgat gagaacggtg gagcgcgccg
     3301 gggccacctg atcgacgagc tggaagatgg cgtgcatccg gtcggagcgg ccgatgatcg
     3361 agccgcgctc gacgctgcgg ctcatcgcgc gccgcagggt ctgtcgctcc tcctccttgc
     3421 ggcgtcgctt gatgccctcg gcaacggtcg agagaatctg ctcgttctgg aagggcttgc
     3481 ggatgcaccc gaaagcgccg cgctcccatg cgccgatggc cgtgtcgaac gtggcgtagg
     3541 ccgtgatcat cacgaccacg ccctccgagt ccgtcttcaa aatctcttcg agcgtgcgca
     3601 ggccgcccat gccgggcatc gacacgtcga gcaggacgac atcgtaggga cggcggccat
     3661 agacctccag cccctcctcg cccgtcttcg ccagatcgac gcgatacccc tcggccgaga
     3721 gcagcgtctc cagcacgtcg cgcatgatct cctcgtcgtc aacgacgaga atggaaccct
     3781 tcttagccat ttctttatgg gtgataagtg atgagtgata agtgataaga aaatcagctt
     3841 ccttcttatc acttatcact catcacttat cacctctcca ttgtactcga ttcgagtcgg
     3901 cgggccgaac gggggttgtc tgagtgggac agggatttct gtccgctagt catcagtcgt
     3961 ctgtcgtcag tcctcagttg tttgcgttgg gcaaatcgcc gctgacaccg aaaaggctca
     4021 ggctgggtga ctcgaaacaa ctgacgactg aggactgacg actgatgact acctgatggt
     4081 gttgcgggcg ttgcgggtcg agaggacgcg gcggtcggaa ggctcgggtg cggggcggcg
     4141 ttgctcggcc gtgacgaaga ggtcgaagtc gtccgactgg aagccggcga cgcggatgta
     4201 ggtgtcggcg cggttgaggg cttcgtccgc cggcaggctg cccatgtaga cgagtcgtcc
     4261 gctcggctga atcgcccaca tgatgtagac gccggcgccc gcctcctgcg gcagcggcac
     4321 gtcgcggaag cgcacgacga cgtaggcgcg gccggcgctc tcggcggcgc gcgccgcccc
     4381 acgcgcacgc ggcgcgctcg acgcgccttc gagttcgatg aggcgaccgc ccccgcgcgc
     4441 gtcgagcgcg ccgtcaacct cggcgtagaa gtctcccgtg gtgcggcgca aacggctact
     4501 gccgccgacc gggtctaacg actcgacgtt gttcctgccg cccggccgtg ggtcagggcc
     4561 gctggcggcg acggtcggcg gggccggcac ggggaagacg gccgtggcct cgccggcgcg
     4621 ggtcgagagg acgggcgcgc cgaggggccg ctcggcgtcg gggctctgct cggcggtgac
     4681 gatgacggtg taacgctcga agcccgccgg gtgattgaag gcgaggccgc cgttgccgcg
     4741 tcggtcgcgg ccgagttcgc cgaggcggac gatgcgcccc tcgctgttgg cccagacgac
     4801 gtaagcccgc gcgtcgcgcg actgcgtctg cgcttcgggc aaccccaatg cggtcaggcg
     4861 gccgcgcccg ccctcggcgc tcggctcgat ggtgagctgg cccgacgccg tcggcgtgaa
     4921 cgagcgcagg cggatgtcca cgcgtccttc ctgcgccggg gcgggccgcg cgtgaggcgg
     4981 tcggcggccg agtgccgaat gcgccgcgcc gacactcaga ccgcccgaag acgacgcggc
     5041 gaaacaaacg gacacgagca gcaggccgaa gggggcacgg gcgcgagtcg tccgccgtcc
     5101 gcaaacattt gtcatgggtg aaagaaccgc cagaagtttt tccgaagtca gagcggaggc
     5161 gagattaaca ccggaagaga gaaatgcgaa acagtttctt tcgtgtgccc gcgctcattt
     5221 tcggttgtgc ggaagggcgg gtatgatgtt tgaagagatg aggagtggcg agtcgatgag
     5281 aagacaggga cgtgcgctcg cggtgctgct gttgtgcgcg atgttgttcg cgcagtcgag
     5341 ctgtcgcggc gacgagggca cgtcggtgtc ggggcgtccg gcgccgcact tcaagccggg
     5401 cttcaacctc ttctcgcccg agcaggacat cgaactcggg gagcagtcgg cgcgacagat
     5461 cgccggcgag atgcctctac tcaacgacgc gaccaccgtc aactacgtcc gcgcgctcgg
     5521 cggcaagctc gcctcgcacg cgccgggcca cgacttcccg tatcagtttc aggtgatcgg
     5581 cgcgcgcgag atcaacgcct tcgcgctgcc gggcggcttc atcttcgtca acgccggggc
     5641 gattgccgcc gcgagaggcg agggcgagat cgccggcgtc atcgcgcacg agatcgccca
     5701 cgtcgcgctc cggcacggaa ccaatcaggc gtcgaaagcc tacatcgcgc aggcgggcct
     5761 cggcgtcctc ggagccatcg ccggcggcgg cgacagtgcg gaggccggtg agatcatcaa
     5821 cgccatcggc ggcgccggcg ccaacatgct cttcctcaag ttcgggcgca cggccgagac
     5881 gcaggccgac ttagaaggcg cgcgcatcat ggcggcggcc ggctacgacc cgcgcgacat
     5941 ggcccgcttc ttcgagacgc tcgccgcgga gggcggcggc ggccagcgcg tccccgagat
     6001 gcgcagcgac caccccgacc ccggcaaccg cgtcgccgct atcaacgaca tcctcccgca
     6061 gctccgcctc ggccccacgc ccacgcgcga cacgcaggag ttccagcagg tcaaggcccg
     6121 actcacgggc gggcaactgc cgcgcgcggc ggccccagcg cgcaccggcc ccggcgaccc
     6181 caacgacatc gagccgggcg cgcgcccccc agcgccttcg gcgaacttcc gcgactatcg
     6241 ttcgcctgac ggctacgtcc gcttaactta tccggataac tgggaggcac tgcgtctgaa
     6301 gggcgacgag gagaatttga tcttcgcgcc gcgcggcgca tacggcgaga tggacgacgc
     6361 ggtctacgtc acgcacggca tcttcgtcgg cttagtgcag ccgcccgcgc gcgaactcga
     6421 agaggccaac cgcgtcttcg tcgagggaca gattcgcgcc aaccccgact tccgcgttcg
     6481 gaaggggccg cagccggtca gcttctctaa cggcagtccc ggctacgcca ccgtcgtcgc
     6541 cggcccctcg accgtgacgg gcgtcgtcga gatcgatgtc atctacacca cggccacgcg
     6601 cgacggccgc ctcttctacc tcatcaccat ggcccccgaa gacgaatacg aacgctacca
     6661 ccccgccttc gagcagatca ttcgcagcgt ccagctcgga cagtaggaag agggcaaggg
     6721 gtaaagggga aggggtaagg ggaagacagg ttcttctccc ttaccccttc ccctttaccc
     6781 cttgcccctg ctcctttatc ccttcccctt taccctttac cccttcagag gtagaggaat
     6841 ccttcgctgt atctgacggc gtggccggag agcttcacgc gttcaccggc gtcttcgcag
     6901 aacagttcgc cgccgcgcgc ggagacctgg agggcgtgaa tctcgcgccg cgagaatttt
     6961 tccgcccagt agggcgtgag cgtgcagtgg gccgcgcccg tcacggggtc ttcggggatg
     7021 ccggcgccgg gggcgaagta gcgcgaggcg cagtcggagg cgctaccggg cgccgtgacg
     7081 atgacgccga gcgagtcgag cgcggcgagc agagtcatgt cggggcgcag cgcgcgcacc
     7141 tcgtcctccg tgtcgtagac ggcgaggtaa tcgaccgagg agcggagcac ttcgcgcgga
     7201 cgtgcgccca atccgtcgag gagcgccgcg ggcgcgtcgc acgctttggc ccgtcgcgcc
     7261 ggcaggtcga gcgtgaggcg ttcgccgtcg ccgcgccaga cgcgcagggg gccgctgcgc
     7321 gagttgaagg tgacggcgtc ggccgacgcg tccgtgaagt tgaagatggc gaaagcggag
     7381 gcgagcgtgg cgtggccgca gaggtcaact tcaatcgtgg gcgtgaacca gcgcaggccg
     7441 taggtgtcct ccgactcgcg cacgaagaag gccgtctcgg agaggttgtt ctcctgcccg
     7501 atcttctgca tcacgtcgtc ggggagccac gcgtcgagcg ggcacacggc ggccgggttg
     7561 ccgccgaaga cacggctggt gaaagcgtcg atgacgtaca ggggtatttt cataaagtca
     7621 ttttaagtgg gaagagacga cgttggaagg gccattatcg caacggcgag ggcagccatt
     7681 gctgatcgcg cggctcggca gtcaatgtgc ccgcagggcg cgcgccacga gacccacgcc
     7741 cccggcgagg agcacgagca tgacaatcgt gccgaacagg ctcgtcggct gaaaatagat
     7801 gtggaggttc atgatgatgc cggcgaagat gatgatgacg ccggtgaaca gcaggagcca
     7861 gccgatcttc gagcggccgt tgtagaacag gaggccgatg ccgaagatga gcggcacgag
     7921 cgacaggccg aaggcgttgt agccccagac gtgccacgac ccgcttgaga cggtgacgct
     7981 gctcgccagc agatacgccc cggcgacggc catcccgagg ccgagcagaa actcccccat
     8041 gccaccgggc gtgccgcccg cgccttccca cttcgatttg ttctcgttgg tatccatcac
     8101 aaaactaagg atgagggatg agggatgagg gatgaagtga aggcaagctg tcttctttca
     8161 tccctcaccc ctcatccctc atccttcatc ctttctcgcc ccgcggctca gccacagtag
     8221 cgccgccccg aagagtccgc accccagaga gtcctccacc gtccaccacc acggcatccc
     8281 cgacgtgtaa tcgatgatga agaacacggg cgcggccagc aggaacgcga cggcgttgaa
     8341 tacgacgagc gggcggaagc gttttacgtc cgtggccaga atccacagag atattccgtg
     8401 catcccgtag gagtaggacg cctgtcggat cat
//
LOCUS       DASVES010000149        18713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_746601, whole genome shotgun sequence.
ACCESSION   DASVES010000149 DASVES010000000
VERSION     DASVES010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746601"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..608)
                     /locus_tag="VF240_05745"
     CDS             complement(216..608)
                     /locus_tag="VF240_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7174767.1"
                     /translation="MSINIKTSGAHHIALRSTNLERSKRFYTETLGFSIIKETPELFL
                     FSAGNTAFGVRGPEADTASGDKFNPHRVGLDHIALACEDETELERVAAALTEAGVENT
                     GVKLDETLGKKYVAFKDPDRIAWEFYMA"
     gene            complement(1290..1526)
                     /locus_tag="VF240_05750"
     CDS             complement(1290..1526)
                     /locus_tag="VF240_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174768.1"
                     /translation="MADKKRWVITASDDRSLKDIKKRVTESGFDIDQVLDEIGCITGV
                     ASDDVAESLRSVPGVADVSPEPSVDIGPPDAPLS"
     gene            complement(1602..2885)
                     /locus_tag="VF240_05755"
     CDS             complement(1602..2885)
                     /locus_tag="VF240_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7174769.1"
                     /translation="MLLAALERGDDSFETGRFLVTFKEGAAEEGLQSLGAQGMRVADA
                     RDFDGQAADLEAVGDADALVFPEIGVAVVGGAAAQERSMSVSAEIAADSPVESIDPEH
                     FVFVAGTPGEYLRGFAQEAEDAPNEYLRGFLRAAETIIKDLREARQPQLQVEEEAAVL
                     GATWGLNACRVPQSARSGAGIKVAVLDTGMDLGHPDFVGRLPAGQTQSFVGQPVQDLH
                     SHGTHCIGTACGPRAPAGSTPRYGIGFRSSIFVGKVLTNSGSSVDGSVLGGMNWAIAN
                     RCEVISMSLSGRQSPVPAYTSAGQKALNNGLLMIAAAGNEALPTGAPANSPTIMSVAS
                     LDSNLRPSSFSNFGKIEIAGPGRDVFSSVPRPTRYGFKSGTSMACPHVAGCAALWAES
                     ASGLRGLALWNRLRATARPLPFSAARVGAGLVQAP"
     gene            complement(3464..3871)
                     /locus_tag="VF240_05760"
     CDS             complement(3464..3871)
                     /locus_tag="VF240_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HEX7174770.1"
                     /translation="MKIQGIVQEGTGDASFWLNKYSDVYQLWTGMTLIPGSLNVRLAE
                     KFDWNDTSIEPFKKIYSLLPYGGNRDICLIPCEIYKEEKDKIYGFAWATTQAANDFDY
                     RVLEIITSVRLREVLQLNNGSVVTIDIPVSWRG"
     gene            complement(4023..5063)
                     /locus_tag="VF240_05765"
     CDS             complement(4023..5063)
                     /locus_tag="VF240_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174771.1"
                     /translation="MLEKIADELLSHQAEDNRLNAAAAGLYSLCSSVTGIVEYSDHAD
                     DSDGTRLPSGEAISPRDAARCVLDHSRTSKFLGGLHAAILEARKRFPDAAIEILYAGC
                     GPFAPLAIPLTTRFSSAEIKFTLLDVHKRSLDAARRIFQALGKSAFVRDYIQCDAASY
                     KHDAPHAIHVVVVEAMQAALEREPQVAITMNLAPQLCPGGMFLPERITVDCYLCDLTK
                     EFPTLPAEADTADSLSGGGRGSVRVNLGRVLELTAGSCRNLLASSNSDEHGGTSLTPK
                     LLNVPEDVDGEFYLMLLTAITVFDSIALDDYESGLTCPRILYDMGKMHGGKVIEFEYQ
                     LGDKPGFKYRFL"
     gene            complement(5266..5715)
                     /locus_tag="VF240_05770"
     CDS             complement(5266..5715)
                     /locus_tag="VF240_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174772.1"
                     /translation="MSMTGFRIGRDDSEYLSVQITSHSHPGSQDYWDGNWVNAEIEID
                     VGSFSGRYDAYLRVEELKDFRDALARLYSFDSNEAKFETMEEQLSISIKGNGLGHFMA
                     ECEALDQAGIGNRLLFSLEFDQTEIPEMLKGLDTVVQEFPVKGKPEI"
     gene            complement(5829..6455)
                     /locus_tag="VF240_05775"
     CDS             complement(5829..6455)
                     /locus_tag="VF240_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174773.1"
                     /translation="MAEANINKMSFQKRLRHLYSLRAEVAALCVVKCRMSHERIIRAG
                     EFMLPVICRLPEGSYQRIGLCSPEVRAKRLGCSHTSARIKLEVAINELNTDDGEGGGQ
                     GTSLGRELNWLNDQIHLLDHRKGEAEKTLAKIEEEIYRAGDRARRNEELIEAQRSVRE
                     MERLHDEYVEQIGALRDRLVKEMDKLIERAGMNQSADAEIGENSDGVK"
     gene            complement(6708..8207)
                     /locus_tag="VF240_05780"
     CDS             complement(6708..8207)
                     /locus_tag="VF240_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7174774.1"
                     /translation="MSTDKFPFLTLNDKTGSAPLYQQIYESIRRAILSGEFTRGGRLP
                     ATRQLAAQLGVARMTVVNAYEQLLIEGYLEGKTGAGTYVAATLPEEMLQIARDGSPRR
                     KTSPASQSHILSRRGKWLAAKTLTELRVQGSGDKLAFQIGLPAVDEFPLHVWSRLAAR
                     RLRRPPRELLSYGDPAGYRPLRASIAAHLQSTRAVQCDVEQVIIVAGSQQALDLTARV
                     LLNPGDAVWVEDPCYPGARNALLAAGAQVVPVPVDGEGFDLAAGLKRRRKARLVYVTP
                     SHQYPLGVTMSLSRRLALLDWARQSGALIIEDDYNSEFRYSGRPLASLQGLDKNGGVI
                     YVGTFSKTTFPSLRLGCMVVPGDLTDAFMAARALIDRHSPSIDQAILTDFIEEGHFAR
                     HIRRMRSLYAERQAQLIAAAASELAGLLEVKPAAAGMHLIGWLPKGVSDNDASEQAAR
                     HRVEAAPLSAYSSKSLARGGLILGYTAVNPRQIKDGARRLARALRRRAG"
     gene            8340..8816
                     /locus_tag="VF240_05785"
     CDS             8340..8816
                     /locus_tag="VF240_05785"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7174775.1"
                     /translation="MRRAIIFAVLMAVTPIIAVGQTSHAPALALKDLRGRALRLSDYK
                     GKVVVLNFWATWCPPCRAEMPDLIKMQREYRSRGLQVVGITYPPEEIAEVRRFVRKLG
                     VNYPIALGTRETKSLFDDTETLPLTVVIDREGKVRERIEGILLPQEFEQKIRPLLK"
     gene            8936..9199
                     /locus_tag="VF240_05790"
     CDS             8936..9199
                     /locus_tag="VF240_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7174776.1"
                     /translation="MLALGASSVLAASKSVTIRVEGMKCNHCSSSVAKALKATAGVEK
                     VEISHEKGEAVIQYDDQKVTEAKLREVINGTGFKAVEEKSATN"
     gene            complement(9613..10017)
                     /locus_tag="VF240_05795"
     CDS             complement(9613..10017)
                     /locus_tag="VF240_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX7174777.1"
                     /translation="MNLTAEKERLLRRDAEWAKAASEGREVESILSYWTEDAVVVPPG
                     LPPVVGKAALRQYVEGSLRIPGFRIYWESKDVTLSPDAQLAYMFSRNEVTMNAPDGTP
                     MTTEGRAVTIWRREPDGEWRCAVDVWNAGPAA"
     gene            10389..10886
                     /locus_tag="VF240_05800"
     CDS             10389..10886
                     /locus_tag="VF240_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174778.1"
                     /translation="MKKDRTSVQFTYERTYTLRHPKFNLFKNVAYLDTAKLSRGPHTI
                     ELGKFAGCTCDCVVTAKISNGMIKGINFPKCESATTVSPKLAKKFEAARKALRKNGQQ
                     KWEDIPVQELTRSSAARARIVVVITTSGDCFEVCIGSGPGTQVCWICCPGWCIGPSDP
                     QVAIF"
     gene            complement(11065..12294)
                     /locus_tag="VF240_05805"
     CDS             complement(11065..12294)
                     /locus_tag="VF240_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7174779.1"
                     /translation="MFRDNFLKSTSGFAVLLFCLSAVWQPMAIVAQSGESIQDLKQKA
                     ADLTKQQKYTEALPILQKLAIAEPNDPETHFYLGFALIAQANVTKDDAARKALRVRAR
                     NAFIRSKELGIQEPVIEALIQNTPADGSDGKTFSQHAEANKLMAEAEALFSQGKLDDA
                     LGNYQKALQLDPKIYEAALFSGDVHNHRGDFQQAETWYQKAIAIDPTRETAYRYSATP
                     LMKQRKYDQARDRYVEAYISEPYNKYSVAGLTQWAQATNTKLAHPAIDIPTNVTFDEK
                     GDAKINLDVSALFGGKDDGSFAWISYGATRSTWRKETFAKTFPQEKAYRHSLAEEADA
                     LRSVVAVATGDKSVKKLSPSMAKLKKLNDEGLLEAYILLARPDEGIAGDHPAYLKRDR
                     DKLRRYVVEYVLTGGGK"
     gene            13114..13629
                     /locus_tag="VF240_05810"
     CDS             13114..13629
                     /locus_tag="VF240_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEX7174780.1"
                     /translation="MSQSPLLDQVRTAVRLRHYSIRTEEAYVRVIRRFILYHKKRHPR
                     EMGVDEIRQYLSHLQPLAEPLRRHLAKIRLQHEEDVRLGCGGVYLPYALGRKYPNAAT
                     DWVWQYVFPAAKLSVDPRTGGRRRRHASEDRLRRAVKRAIRDAENRQDCCTASPNTNA
                     SNESRPHVKKV"
     gene            13793..14497
                     /locus_tag="VF240_05815"
     CDS             13793..14497
                     /locus_tag="VF240_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7174781.1"
                     /translation="MMSLEASSMRKVLIVEDDQAMAVALRDGFTYEGYAVNVARDGQT
                     GLRMASEKDHDLLILDVMLPRLSGLDVCRQLRGAGNTTPIIMLTARGQEIDKVLGLKT
                     GADDYVTKPFSFLELMARVEAVLRRASKPAEAVESFEFGDVALNFKTLEATKDGRALE
                     LSPREFKIMRYFVEHRGEVVSRDQLLDHVWGYEGLPLTRTVDMHIAKLRQKIEDTPGD
                     PRYIITVHRVGYKFTG"
     gene            14603..15823
                     /locus_tag="VF240_05820"
     CDS             14603..15823
                     /locus_tag="VF240_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7174782.1"
                     /translation="MSTSLKAARAAVAVCLVCLAPALASAQGPDPTRQRVVYKHPDAD
                     KVSVRQDVTYKTVGTAALKMDVYTPPAAKAGERLPVVIFVNAFGDPPGRAKVKTWGQY
                     TDWPRLVAASGMVGVTYEARPDPAASNHDLAALIDYVRASAADLHVDAERVGLWSCSG
                     NVLMGLPVALDETRKYIRAAVFYYGVMNEHPTRNDVPFFVARAGDDDPQLNGSIDVFM
                     KAAIEEEVPITFVNYVGAQHAFDLVDDTERTREIIRQTIDFLKFNLTRNYDAEEAARR
                     VLSPPKFMAMIQRQGIQKAVQEFEAQRKSNPNAAIYQENTLNTLGYRLLQQGKVKDAV
                     EVFKLGVASYPNSANTYDSLGDGYEADGQKELAIQMSEKTLQVLAAQTGLSEDQKNNI
                     RTSAEAKLKRLKGN"
     gene            16153..17304
                     /locus_tag="VF240_05825"
     CDS             16153..17304
                     /locus_tag="VF240_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7174783.1"
                     /translation="MKFDEPEPETEFTPDVEEFFRRRDEAMREYVEALPVMSPAAISD
                     ELEQLGYKGQDEQRRAVSLMAYRHVRRLRRLHVDGEPQRALPPKQNVLMVGPTGCGKT
                     FMVELLFRHVLKLPTVVVDVTSFTESGYVGDDVRTILTRLISAAGDNSDLAECGIVCI
                     DEFDKLASSSSNARFAGQGTTKDVSGYGVQRELLAMLEGATVPVPMDYGFSAYGQRVE
                     LSTRNIPFIACGAFSGLDEMLKARRGNIGFRGGGDGEDPTTLTLDEVESFQKYGFLPE
                     LIGRFSRIVVFQPLPAETLRRILSENVLPQFEGEFRAEGLRLTVTEDALAHIVARSQR
                     RGTGARGLNTELIAAVERAAFETFARTTNAEVIITLADGRLESEVRKLA"
     gene            17428..18150
                     /locus_tag="VF240_05830"
     CDS             17428..18150
                     /locus_tag="VF240_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174784.1"
                     /translation="MNSLSLHSSSVKEAVDLESEVARLEAALAGRRAELTALQDGLRE
                     FKTRYAQVVGSRLAELAEVEKAIREAEGRVLGVEREPEEEEAETDFYGGQDASAAPVG
                     KSLRRLFWSVAKMFHPDHAADEGEARRRHTVMAEASRAYREGDVESLHTLLGDEGLQS
                     FCTGAQPDEDPEDLGARALRLELELRTIEFGIKRIRLDALYRLKLEIEAEAARGRDAL
                     TLSAERIDRQIVKARRRLENLS"
     gene            18413..>18713
                     /locus_tag="VF240_05835"
     CDS             18413..>18713
                     /locus_tag="VF240_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174785.1"
                     /translation="MYTSDTPPARKRLIKYLVFKSVIDLLFVGGLAVGFYLDAFNPSL
                     RGSLDEAGARGVAGWVVDKSNPAAHVEVQLYVNGRFVESRQADFGRADLVAAGVAE"
BASE COUNT         3897 a         5617 c         5355 g         3844 t
ORIGIN      
        1 tcttgcgcct gggccgtatg tccgctcagg tacgaggaca gcgcgaggag tatgcctgcg
       61 acaattgttc taatcctcat tccccctccg ccaaaagaag cagccgaacg cctgagctaa
      121 gctgcccgcc gccaaattaa ccttgaataa atgacaagat tgttggcggc gggtcagctt
      181 gagcgagttg ttaggcacgg cgtgaggggc caactctatg ccatgtagaa ctcccacgct
      241 attcggtcag ggtctttgaa ggcgacatac ttcttgccca gtgtctcgtc gagcttgacg
      301 cctgtattct ccacacccgc ctccgtgagc gcggcagcca ccctctctag ctcagtctcg
      361 tcttcgcagg ctaaggcgat gtgatccaaa ccgacccggt gcgggttaaa cttatccccg
      421 ctcgccgtgt cggcttcagg gccgcggaca ccgaatgcgg tgttccctgc actgaagagg
      481 aacaattcag gggtttcctt gatgattgag aagccgagcg tctcagtgta gaagcgcttt
      541 gagcgttcta agttggtgga gcgcaaggca atgtggtgcg ctcctgaggt cttgatgttg
      601 atgctcataa cccatctcgc tttctgttgc agatcgcatg cacttctttg tcggaacacg
      661 atacagcagg caaggaactt cgccaaccgg atgacgatta gagaggttag cgccgaacgc
      721 cagaattaac ccgccggacg acatctgcgg cacgagcaag ttctcgatga gagccgagct
      781 gattccggtc gggctgaatg agtcgttggg cagccgtcgc gacgtaacta ttcaacttcc
      841 gatgaggatt tgattcgctc cgtgagattc aattcgactc tcttgtttcg cgcagcggcc
      901 caactactat tgtgccgaat gtgtcggcct gaccacgtaa tagagcggca aaagccgaat
      961 cattccgtcg aactacgtaa cggggaggca aaagccgaat ggcccggcat aacacccgcc
     1021 gcaattaggg gaagatattt gagagggggc ggggtgtcaa cggtttgttt tgtggagggg
     1081 agagtttgcg aggggagggg tgtcaaacgg ccagactgaa acgggcggcg aggcgctcgc
     1141 cgctctacac gatgaggctg agcgacgtgc ccgttaccgc gttggttgct atcgaggcgg
     1201 aacttactca cagaacaccg ccagccctac cgaaagaatc aaggctccga ggcggccgtg
     1261 atcgcagtct taaaagttca taacgggctc taggacagag gcgcgtctgg aggtccgatg
     1321 tcaacggacg gctccggcga aacgtcggcg acgccgggaa ccgaacgcag gctctcggcg
     1381 acatcgtcgc tggcgacccc tgtgatgcac ccgatctcgt cgagcacttg gtcaatgtcg
     1441 aagccggact cggtcactct tttcttgatg tctttgagcg accgatcatc cgacgcggtg
     1501 atgacccatc ttttcttgtc ggccatagaa tgcctccgtc agctgaaatg tacccgccgg
     1561 gaagattctc ccggcgggta ctattctagc gcacaagggc tttatggcgc ctgtactagc
     1621 cccgcgccga cacgcgccgc cgagaagggc agcggccggg ccgtcgcccg cagcctgttc
     1681 cacagtgcca agccccgcag accggatgct gactcggccc acaacgcggc gcagccggca
     1741 acgtgcggac aggccatgct cgtgccggat ttgaagccgt accgggtcgg tcgtgggacg
     1801 gacgagaaga catctcggcc cggcccggca atctcgatct taccgaagtt ggagaacgac
     1861 gatggtcgga ggttcgagtc gagcgaagcc acggacatga tagtcggtga gttggcgggc
     1921 gcgcccgtgg ggagcgcttc gttgcccgcc gcggcgatca tcaggaggcc gttgttcagc
     1981 gccttctgtc ccgccgatgt gtaggcgggc accggcgatt gtcgcccgct cagcgacatc
     2041 gaaatcacct cgcagcggtt ggcaatcgcc cagttcatgc cgcccaggac gcttccgtca
     2101 acgctcgacc cggagttagt cagcaccttg ccgacgaaga tggaggagcg gaagccgatg
     2161 ccgtagcgcg gtgtgctgcc cgccggtgct ctcggcccgc acgccgtgcc gatgcagtgc
     2221 gtgccgtggc tgtggaggtc ttgtacgggc tggccgacga agctctgtgt ttggccggcg
     2281 gggaggcggc cgacgaagtc cgggtgcccc aggtccatgc ccgtgtcgag cactgccacc
     2341 ttgatgcctg cgccgctgcg cgcgctttgt ggaactctgc aggcgttaag cccccacgtc
     2401 gcgccgagca ccgccgcttc ttcttcgacc tgcaactgtg gctgtctggc ctcgcgcaag
     2461 tctttaataa tcgtctcggc ggccctgagg aaaccacgta ggtattcgtt gggggcgtct
     2521 tcggcctctt gcgcgaagcc gcgcaggtac tcgccgggcg tgcccgccac gaaaacgaag
     2581 tgctccgggt caattgattc gaccgggctg tcggcggcaa tctccgcgct gacgctcatg
     2641 ctgcgttcct gggccgccgc gccgccgacg accgccacgc cgatctccgg aaagactaag
     2701 gcgtcggcgt ctccaaccgc ttccagatcg gcggcttggc cgtcgaaatc tcgcgcatcg
     2761 gcgacgcgca ttccctgtgc ccccaacgac tgcaacccct cttcggccgc gccttccttg
     2821 aaggtgacga ggaaacggcc cgtctcgaag ctgtcgtcgc cacgttcgag cgcagcgagg
     2881 agcatttcat cgatggggga cgacgtacga gtggcggcgg ccccggctgg ggatgccgcg
     2941 cgacggatag actttctacc cgctggccta ctacgacctt tctttgttgc catcactgtt
     3001 cctccaagag tttggtgact tcccaaaagc gcggactatc agcctgaggt ttagagaaga
     3061 gttcttgccc accacagggc gaaatcgaaa aggaaagtag cgggtcactt ccaaatctga
     3121 cgaggactgc tctcccgtta acccatccag agataattgt ccggcctact gttctctcaa
     3181 tatgaatgcc atcttggggc aaaacatttc atcgggtcgc ccgcgcacgg aggaagcagc
     3241 aaggagcgca ctcgcttttg aggttgattc tgcttttgtg caaaggttcg catattctat
     3301 ctcctgttca gctgcagcgg agctgcctca cgcccggcat cacccgccgc gcacacaacg
     3361 ccgaatcgcg ggcgtcttgc ggatgagagc cgcgctattc gcagtcgggt gcatgccatt
     3421 gtcaggcgcg gctaagtaag aacgactcat catttaataa atgtcagcct ctccatgata
     3481 ccgggatgtc gattgttacc accgacccat tattcaactg caagacctca cgcaatctaa
     3541 ctgacgtgat aatttcaagc actctataat cgaagtcatt tgccgcctgt gtggtcgccc
     3601 acgcaaaccc ataaatttta tctttctcct ctttgtaaat ctcacagggg atcaaacaaa
     3661 tatctcgatt gccgccgtag ggaagtagcg aatatatttt tttgaatggc tcgatgctcg
     3721 tatcattcca gtcaaacttt tcagccaaac ggacattgag tgagcctgga ataagtgtca
     3781 tccctgtcca taattggtaa acatcagaat atttgttgag ccaaaatgaa gcatcccctg
     3841 tcccctcttg cacaattccc tgtatcttca ttgttttaat ttctatagac gcctaatgcc
     3901 cggcatcacc cgccgcgcat tcaacgtgag aacgggcaag ttctcgatga catgctcgct
     3961 gaatcgcggt cgggttgcat gccgttgtca gacggatcgc tgccggcatc tgctcaccac
     4021 ccttataaaa acctgtattt gaatcccggc ttatcgccaa gttggtactc aaactctata
     4081 acctttcccc cgtgcatctt gcccatgtca tataaaattc tcgggcaggt caatccagac
     4141 tcgtaatcat ccagcgcgat agagtcgaag acggtgatgg cggtcaagag cattaagtag
     4201 aactccccgt ccacatcctc cgggacgttg aggagttttg gggtaaggga tgtcccacca
     4261 tgctcatcgc tgttgctgga tgctaacagg ttacggcaac tcccggcagt caactcaagc
     4321 acacggccaa gattaaccct gacgctaccc cggccgcccc ccgacaaaga atccgccgta
     4381 tcagcctcag cgggaagagt cggaaactct tttgttaaat cgcagagata gcaatcgaca
     4441 gttattcttt ctggaaggaa catccctccc gggcaaagct ggggggcaag gttcatcgtg
     4501 atagccacct gcggctctct ttccagagcc gcctgcatag cctccacgac tacgacatgt
     4561 atggcgtgag gcgcatcgtg cttataggat gcggcgtcac attggatgta atcacgcacg
     4621 aaagcgcttt tcccaagggc ttgaaaaata cgccgcgccg catccagaga tcgtttgtga
     4681 acatcgagca gggtgaactt tatctcggca gagctgaacc gggtggtcag tgggatggcc
     4741 aggggcgcga aagggccgca ccccgcgtaa aggatttcta tcgcggcatc agggaacctc
     4801 ttccgtgctt cgagtatcgc agcatgtaag ccgcccagga atttagaggt tcgactgtgg
     4861 tcaagtacac atctggcggc gtccctgggg gagatggctt ccccgctcgg aagcctggtc
     4921 ccatccgagt cgtccgcgtg atccgaatat tcaactattc cagtgacgga tgagcatagt
     4981 gaatacagcc ccgccgccgc cgcatttaat ctgttgtcct cagcctgatg agatagcaat
     5041 tcatcggcaa tcttctccaa caagagtgaa taaccatctg ccgaagttgg ccgttgaaaa
     5101 atgttcatta gcttttatct taattgtctt caaaagactg tagttcatgc gtgcatgcgt
     5161 ggttgccgtc taacgcccgg catcacccgc cgcgcacaca acgcatcaag cacggcgtct
     5221 cgcggatgga agccgcgcta ttcacggtcg ggtgcatgcc gggcgttaga tttcgggttt
     5281 acccttaacc gggaactcct gcactacagt atctagtccc ttcagcatct caggaatctc
     5341 tgtctgatcg aactccagag aaaagagaag gcgattgccg atgcccgcct gatctaatgc
     5401 ctcgcattca gccataaagt ggcccaagcc gttccccttg atgctgatgc tcaactgctc
     5461 ctccatcgtt tcaaatttgg cctcgttaga gtcaaatgaa tagaggcgag cgagcgcgtc
     5521 acggaaatct tttaattcct cgacacgaag ataagcatca taccgaccgc taaaactccc
     5581 cacatcaatc tcaatctcgg cattcaccca attaccatcc caataatcct ggctgccggg
     5641 atgagagtga cttgtgattt gcacgctcag gtattcagaa tcatccctgc cgatacgaaa
     5701 gccagtcata ctcataccga acacgaagaa atctaacgcg cgaatttaac cggcgagcaa
     5761 tcaacttgaa gataaaagag ccgccctatt gcgagtccgg ttgaatgagt tgttagaccg
     5821 ctgccccgct acttcactcc atcgctattc tcgccaatct ctgcatctgc gctctgattc
     5881 atacccgctc gctcgatcaa cttatccatc tctttcacaa gtctatctct caacgcacct
     5941 atctgttcca cgtactcatc atgtaatcgc tccatctcgc gaacagacct ttgggcctca
     6001 atcagttcct cgtttctgcg cgctcgatca ccagcgcgat atatctcctc ctctattttg
     6061 gcaagggtct tctccgcctc accctttcgg tgatctaata ggtgaatctg atcgttcagc
     6121 caatttaact ccctgcctaa tgatgtgccc tgcccgccgc cctctccatc atctgtattc
     6181 aattcattga tagcgacctc tagtttaatt ctggcagaag tatgactaca gccgagcctt
     6241 ttggcacgta cctcgggact acataggcca atcctctgat agctgccttc cggtaagcga
     6301 caaattacag ggagcatgaa ctcacccgct ctgataatcc gctcgtggga catgcggcat
     6361 ttcacgacgc ataaagcggc gacctccgca cgcaaggaat aaagatggcg aaggcgtttc
     6421 tggaagctca tcttatttat gttcgcttct gccactctcc gcggtctaac gcccgcatta
     6481 accggcgcgc aatcaatgtg aaagatataa ggcttgctga ttgcgagtcc ggttgaatga
     6541 gttgtcagac agcggggatc gcagccgcgg tgtatgaagc aaacctccgg gctagttgcg
     6601 cggttgtagc ttcaacatgc gcgatcactt tgcctttctg aaggtactgc cccttgcctt
     6661 taggccatcc ttgccttccc atcggtaatt ctcatcacct ggactcatca ccctgccctc
     6721 cggcgcaatg ctcgcgccag tcgtcgtgcg ccgtccttga tctgcctggg gttgaccgct
     6781 gtgtagccga gaatcaatcc gccgcgcgcc aatgatttgg aactataggc tgagagcggc
     6841 gcggcctcca cgcggtggcg cgccgcctgc tccgacgcgt cattgtcgct cacacctttc
     6901 ggtagccagc cgatcaaatg catcccggcg gcagcgggct tcacctccag aagccccgct
     6961 aactcgctgg cggcggcggc gatgagctgc gcctgacgct ccgcgtacag agagcgcatc
     7021 cggcggatgt gacgtgcaaa gtgtccctcc tcgatgaagt ctgtcagtat cgcctgatca
     7081 atcgagggag aatgcctgtc gatgagcgcg cgggcggcca taaaggcatc cgtcaaatct
     7141 ccggggacga ccatgcagcc cagacgcagg gagggaaagg ttgtcttgct aaacgtgccg
     7201 acgtagatta ccccgccgtt tttgtccaac ccttgcagcg atgcgagcgg gcgtcctgaa
     7261 tacctgaatt cactgttgta gtcgtcttcg ataatcaatg cgccggattg cctcgcccag
     7321 tcgagcagcg ccaagcggcg agataagctc atcgtcacgc cgagtggata ctgatgcgag
     7381 ggcgtgacgt aaaccagccg tgccttccgg cgccgcttca acccggcagc taaatcaaag
     7441 ccttcgccat ctaccggcac ggggacgacc tgcgctcctg cggcgagtag cgcattgcgc
     7501 gcgcctgggt aacacgggtc ttccacccag acggcatcac cgggattgag aaggacgcgc
     7561 gccgtcagat cgagtgcctg ctgcgagcct gcgacaatga ttacttgctc gacgtcgcat
     7621 tgcaccgccc gcgtcgattg taagtgcgcg gctatgctcg cgcgcaacgg gcggtagcca
     7681 gccggatcac catagctcaa cagctcgcgc ggcggacgcc gcagccgtcg ggccgccagg
     7741 cgcgaccaca cgtggagggg aaattcatca acggcaggca agccgatctg aaaagctaat
     7801 ttatctccgc tcccttgcac gcgcaactcc gtcaaagttt tcgctgccag ccactttccg
     7861 cgtcgggaga gaatgtgact ttgcgacgca ggtgaagttt tgcgacgggg cgagccatca
     7921 cgagcaatct gtagcatctc ttccgggagc gttgccgcga cgtatgttcc agcgcccgtt
     7981 tttccttcga ggtagccttc gatcagcagt tgttcgtagg cgttgacgac cgtcatccgc
     8041 gccacgccta attgtgccgc cagctgccgg gtcgccggca gccgccctcc gcgggtgaat
     8101 tcaccgctta gaatcgctcg gcgaatcgac tcgtaaattt gctggtacag cggcgcggag
     8161 ccggttttat cattgagcgt aaggaagggg aatttgtcgg tgctcataat tttgtaatga
     8221 taaattggtc tatatatttt gtcgaatagt ggctcttgcg cagagaccaa atatgcctta
     8281 aattcgtttg ggaaacagcc ctgccgggac ttgaagtatc tatttattga ggtgaagggg
     8341 tgaggagagc aatcattttc gccgtgctca tggccgtgac gccgattatc gcggtagggc
     8401 aaacgagcca cgccccagcc ctggcactga aagacctgcg cgggcgcgcc ctgcggctga
     8461 gtgactacaa aggcaaggtc gtggtgctga acttctgggc gacgtggtgc ccgccctgcc
     8521 gcgccgagat gcctgatttg atcaagatgc agcgcgaata tcgaagccgg ggattgcagg
     8581 tcgtcggcat tacttaccca ccggaagaga tagcggaagt tagacggttt gtcaggaaac
     8641 tcggggtcaa ttatccgatt gccctgggca cgagagagac caagtctctc ttcgatgaca
     8701 ccgagacgct gcctttgacg gtcgtcatag atcgggaggg caaggtccgt gagcgcatcg
     8761 aggggattct tttaccacag gagttcgagc agaagatcag acctttgctc aagtaagccc
     8821 ggatatagaa tgcacatcgg agtattgctg atccggtttt catcaacccc acagattcaa
     8881 ggagatgtag agaatgcgta aaagtagagt tgccaccctg ttaggaatag ttctcatgct
     8941 ggcactgggc gccagcagcg tgttggctgc cagcaagtcc gtcaccattc gcgtcgaggg
     9001 catgaaatgc aatcactgct cgagttccgt tgccaaagct ttgaaagcaa ctgctggtgt
     9061 cgagaaggtc gagatcagtc atgaaaaagg tgaggcagtt atccagtatg acgatcagaa
     9121 ggtgacggaa gccaaactgc gggaggtcat caacggcacg ggcttcaagg ctgtcgagga
     9181 aaagtccgcc acgaattgaa aagagcgaac cctctcattt acagagttta ataaataatt
     9241 aaagggagtt catttgctcc ctttaattat tagatggcca tctcggaaat cgctgaggca
     9301 tgattcaacg gcttcatgcg ccttctggtc gtggcgggct tggcgcaaac gtgtctcggt
     9361 tcgctctgac agttgtttga ggtcaagacc acgatgctcg actgcccatt cgagattcgc
     9421 ataatggtca gaaatctctc gcttcaactg agcgaaatcc tccagccagg cattatttgc
     9481 attctccgag acatggtttc ctctaaagag aagctgtcta acgctcggca tcacccgccc
     9541 gcgcgagccg tgtgaagcat ccaaccttgc gaatggcacc cgcgctgcat cgggtcgggt
     9601 gcatgccgag cgttaggccg caggcccggc attccagaca tccacggcac accgccattc
     9661 cccatcaggc tctcgacgcc agatcgtgac cgcacgcccc tcggtcgtca tcggcgtacc
     9721 gtcgggcgcg ttcatcgtca cctcattgcg gctgaacata taggcgagtt gtgcatcggg
     9781 tgaaagcgtg acatccttcg actcccaata aatcctgaat ccgggaatcc gcaaactgcc
     9841 ctcgacatac tgacgcaacg cggccttgcc gacgaccggc gggaggccgg gaggcacgac
     9901 gaccgcatcc tcagtccagt acgagaggat gctctcaacc tctcgcccct cggaggccgc
     9961 cttcgcccat tcggcatcac gccggagaag gcgctctttt tccgcggtta agttcactca
    10021 aacctccctt ctgattacac agacccctgg gcggcctaac gccaggcatc acccgcgccc
    10081 aatccaagat gcatgaaagg cagcgtggag gggcgtcggg tgcatgccgt cgttagatgg
    10141 tgcgtcatcg gagccattca actatcgcct atgcctacgc ggcgcgcgcg gccccgaaca
    10201 cataacgccc ctggtgcgac accaagcttc cgctccacag tgcagatcgc aaactctcag
    10261 agtcgctcct agatgaactg tattcactct atacgaggag actgaatgat ggtatattcg
    10321 caggcgcttg tgaagtcaaa aaggatattc ccggatgccg cccggagttt gttttggagg
    10381 aatgagagat gaagaaagac cgaacttctg ttcagtttac atacgaacgc acatacaccc
    10441 ttcgccatcc aaaattcaat ctgttcaaga atgtcgcata tcttgatacg gctaagctga
    10501 gcagggggcc gcatactatt gaacttggaa aattcgcggg atgcacctgc gattgcgtgg
    10561 tgacggccaa aattagcaac gggatgataa aggggataaa ctttcccaaa tgcgagagcg
    10621 cgacgacggt ctcgcctaaa ctcgccaaaa aatttgaggc ggcacgcaag gcattgagaa
    10681 aaaatggtca acagaaatgg gaagatattc ctgttcagga actaaccaga agttcagccg
    10741 cgcgcgcgcg aattgttgtt gttatcacaa caagcgggga ctgttttgag gtgtgtatcg
    10801 gttccggccc agggacacag gtctgttgga tctgttgtcc cggctggtgt ataggcccat
    10861 cagacccaca ggttgccatc ttctaacctt tttcttctaa gccaggtact cttatccgta
    10921 agtacgatga catctaacgc ccggcatcag cggcgcgcac agaacttgat cttgagaggg
    10981 ccgctgtggt gcgcgtccgc cgcatgccgt tgttagatgc tgcctctatt aggccaagcc
    11041 atcgaccatt tgctctccaa ggctctattt cccgccgccg gtcaaaacat attcgacgac
    11101 ataccggcgc agcttgtcgc ggtctcgctt gagataggcc ggatgatccc cagcgatgcc
    11161 ctcgtcaggt cgagccaata agatgtaggc ttcgagcaaa ccttcgtcgt tgagcttttt
    11221 caatttcgcc atcgaggggc taagcttctt cactgacttg tcgccggtgg cgaccgcgac
    11281 gacgcttcgc aacgcgtcag cttcctccgc caggctgtgg cggtatgctt tctcctgcgg
    11341 aaaggttttt gcgaaagttt ccttgcgcca agtcgagcga gtcgcgccgt aggagatcca
    11401 tgcgaaagag ccgtcgtctt tgccgccgaa cagagcactc acgtctaaat tgatcttcgc
    11461 gtcgcccttc tcgtcaaaag tgacgttggt cgggatgtct attgccgggt gtgcgagctt
    11521 ggtgttcgtc gcctgcgccc attgcgtcaa gccggcgacg gaatatttgt tgtagggttc
    11581 actgatgtat gcctcaacgt aacgatcacg tgcctggtcg tacttgcgtt gcttcatcaa
    11641 aggcgtcgcc gaatatcggt atgccgtctc cctggtcggg tctatggcaa tggctttctg
    11701 ataccacgtt tcggcctgct ggaagtcgcc gcgatggtta tgcacgtcgc cggagaaaag
    11761 cgcggcttca tatatcttcg gatcgagctg tagtgccttt tggtaattcc cgagcgcgtc
    11821 gtcgagcttg ccctgcgaga aaagggcttc ggcctccgcc atcagtttgt tcgcctcggc
    11881 gtgttgcgag aacgtcttgc cgtccgagcc gtctgccggg gtgttctgaa taagagcttc
    11941 tatcaccggt tcctgaatgc ccaactcctt ggacctgata aaggcgttgc gggcgcgaac
    12001 gcgcaatgct ttacgcgccg cgtcatcctt cgtgacattc gcctgcgcga ttagagcaaa
    12061 tccgagataa aaatgtgttt cggggtcatt gggttcggca atcgcgagct tttgcaaaat
    12121 cggcaaagct tctgtgtatt tttgctgttt ggttaagtcg gcagcctttt gcttgaggtc
    12181 ctgaatgctc tcgcccgact gggctactat cgccatgggc tgccaaaccg ccgacagaca
    12241 aaataacaaa accgcgaagc cagatgtgct tttgagaaag ttatctcgaa acatattaac
    12301 tccttagcaa agatatcacc gttgaagacg gaagcgtcta acgcccggca tcagcggcgc
    12361 gcacgaacca ccgatgataa gaggtacgct gggcgcgcgt ccgctgcatg ccgttgttag
    12421 atttcgccac aaccactccc ccgcatcggc atttgctttc tttctgccgc aggaataaac
    12481 cgccacccct ttgactcatc aacgtcaaat gcgccgacaa aaacactgcc cttcgggtta
    12541 acgcattctg agcttacctc tctgatgata tgccaggcgg ggtctacctc atacctgccc
    12601 attctgtctt gcacgatgag catggaatca gcccttcgga agaagacgtt cgcgcggctc
    12661 aacccgccgg tatcggtaga gatttgcacc cgattgactg gcctacctcc gacattcagc
    12721 acgagaagac ggtcatactc ggctaggaaa tcgtgaatgc agacgcgctc aagctgtaag
    12781 gaagcgccct cccctgcgat acgaagggac gcagatgagc gattgccttt cgcgctacaa
    12841 gcagcgcaga ttagcaggct caagctgagg agcaataatt ctttcttccg catcattacg
    12901 actctccacc gagaaatcta acgctcatta gaccgccact ggcggtactt tacaactatt
    12961 gccgaccttt accgccactg gcggtctaat accaggaaag ccgacaaaca tcgcgcgtgg
    13021 cggcctatta cgtctccggg ctgacttgta tcgccgcctg gcggatcatc cccgcgctct
    13081 tcccactccg cacctcacgt ggaggttctt cacatgtctc agtcgcccct gctcgaccag
    13141 gtgcgtaccg ccgtccgcct acgccattac agtatccgca ccgaggaggc ttacgtgcgg
    13201 gtcatccgcc gcttcatcct atatcacaag aagcgccacc ccagagagat gggcgtggac
    13261 gaaatccggc aatatttatc acacctacag ccgctcgccg aaccgctccg gcgccacctt
    13321 gccaaaatcc ggctacagca tgaagaggac gtgcggctag gctgcggcgg ggtctacttg
    13381 ccttacgcgc tggggcgtaa atatccgaac gccgcgaccg attgggtgtg gcagtacgtc
    13441 ttccccgccg cgaagctgtc ggtagacccg cgcacgggcg ggcggcggcg gcgccacgcc
    13501 tccgaagaca ggttgcggag agccgtcaag cgggcgattc gggatgcgga aaaccggcaa
    13561 gattgttgta ccgcttcacc caatacaaat gcctctaatg aaagccggcc ccatgtaaaa
    13621 aaagtgtaaa agggccggtt gagatataaa atatctgtaa aaaaggttcc gggtcctgtt
    13681 gatttctgta tggtatgtgc ttaggttaca ttggcgctct cagcaagagt cagcgtgtag
    13741 tttctgcaaa caacttgaac aagacgttcc acgccgagcc gaaggcaaaa aaatgatgtc
    13801 gctcgaagcc agcagcatgc ggaaagtcct gatcgtcgaa gacgatcagg cgatggccgt
    13861 cgccctgcgc gacggcttca cctacgaggg ctacgccgtc aacgtcgcgc gcgacgggca
    13921 gacgggcctg cggatggcct cggagaagga tcacgacctc ctcatcctcg acgtgatgct
    13981 gccgcgcctg agcggcctcg acgtgtgccg gcagttgcgc ggggccggca acaccacccc
    14041 gatcatcatg ctcacggcgc gcggtcagga gatcgacaaa gtcctgggcc tcaagaccgg
    14101 cgccgacgac tacgtcacca agcccttcag cttccttgag ctgatggcgc gcgtcgaggc
    14161 cgtcctgcgc cgcgcgtcga aaccggccga ggcggtcgag agtttcgagt tcggagacgt
    14221 tgcgctgaac ttcaagacgc tcgaagccac gaaggacggc cgggcgctgg aactctcccc
    14281 gcgcgagttc aagatcatgc gctacttcgt cgagcaccgc ggcgaggtcg tcagccgcga
    14341 ccaactgctc gaccacgtct ggggctacga gggcctgccg ctgacgcgca ccgtggacat
    14401 gcacatcgct aagctccgcc agaagatcga ggacacgccg ggcgacccgc gctacatcat
    14461 cacggtgcat cgcgtcggct acaagttcac gggataaaaa cagaggggaa ggggtaaagg
    14521 gtaaggggga agggtgggct tcgtctcttc ccctttaccc cttccccttt gccctttccc
    14581 cttgcttcag gaggtgtctc ccatgtcaac aagtctgaaa gccgcgcgcg cggccgtggc
    14641 cgtctgtctg gtctgtctcg cgccggcgct tgcctccgcg caggggcctg acccgacgcg
    14701 ccagagggtc gtctacaaac atccagacgc cgacaaggtc tctgtgcggc aggacgtgac
    14761 gtacaagacg gtcggcacgg ccgccctcaa gatggacgtg tacacgccgc ccgccgcgaa
    14821 ggctggggag cgcctgcccg tcgtcatctt cgtcaacgcc ttcggcgacc cgcccggtcg
    14881 ggcgaaggtc aagacgtggg ggcagtacac cgactggccc cggctcgtcg ccgcctccgg
    14941 catggtcggc gtcacctacg aggcccgccc agacccggct gccagcaacc acgacctcgc
    15001 ggccctgatc gactacgtgc gcgcaagcgc cgccgacctc cacgtcgatg cggagcgcgt
    15061 cggcctctgg tcgtgctcgg gcaacgtcct gatgggcctg cccgtggcgt tggacgagac
    15121 gcgcaagtac atccgcgccg ccgtcttcta ctacggcgtg atgaacgagc acccgaccag
    15181 gaacgacgtg cccttcttcg tcgcgcgcgc cggcgacgac gacccgcagc tcaacggctc
    15241 catcgacgtt ttcatgaagg ccgccatcga agaagaagtg cccatcacct tcgtcaacta
    15301 cgtgggcgcg cagcacgcct tcgacctcgt ggacgacacc gagcgcacgc gcgagatcat
    15361 ccgccagacc atcgacttcc tgaagttcaa cctgacgcgc aactacgacg ccgaagaagc
    15421 cgcccggcgc gtgctctcgc cgccgaagtt catggcgatg attcagcggc agggcatcca
    15481 gaaggccgtg caggagttcg aggcgcagcg caagtcgaac ccgaacgccg ccatctatca
    15541 ggagaacacg ctcaacacgc tcggctatcg cctgctccag caggggaagg tgaaagacgc
    15601 cgtcgaggtg ttcaagctgg gcgtcgcctc ctacccgaac tcggccaaca cctacgacag
    15661 cctcggcgac ggctacgaag ccgacggcca gaaggagctg gccatccaga tgtccgagaa
    15721 gacgctgcaa gtgctcgcgg cgcaaaccgg gctgagcgaa gatcagaaga acaacatccg
    15781 cacgagcgcc gaggcgaagc tgaagcgcct gaaagggaat taacccgcgc cgccagcgga
    15841 cgcgtcaaag agcgccgaag gcgcggcagt tattagcccc gcccgtgagg gcggggtaca
    15901 caggccgaat agaaattcga gccccgaagg ggcggcacgt ccgaccgacg cggaaatgtg
    15961 ccgccccttc ggggctcaat tttcattgcc gactcgttaa ccccgccctc acgggcgggg
    16021 ctattaactg ccgccgcttc gcggctcgtt gaactattgc cgcttcgcag ctcgtgcggc
    16081 tccaaacact tcgcggctcg ttcacgcgcc gcgcgttgac gcactgcgcg accttcaagt
    16141 taaattgtcc gcatgaagtt cgacgagcct gaacccgaga cggagttcac cccggacgtt
    16201 gaggaattct tccggcgacg cgacgaggcg atgcgcgagt acgtcgaggc gctgcccgtg
    16261 atgtcgcccg cggccatctc ggacgagctg gagcagctcg gctacaaggg gcaggacgag
    16321 cagcggcgcg ccgtctcgtt gatggcctac cgccacgtgc gccggctgcg tcggctccac
    16381 gtcgatggcg agccgcagag ggcgctcccg cccaagcaga acgtgctcat ggtggggccg
    16441 acgggctgcg gcaagacctt catggtcgag ctgctgttcc ggcacgtcct gaagctcccg
    16501 accgtggtcg tggacgtgac gagcttcacc gagagcggct acgtcggcga cgacgtgcgg
    16561 accatcctca cgcgtctcat ctcggcggcc ggcgacaact ccgacctcgc cgagtgcggc
    16621 atcgtctgca tcgacgagtt cgacaagctg gcctcctctt catcaaacgc gcgcttcgcc
    16681 gggcagggaa cgaccaaaga cgtttcgggc tacggcgtgc aacgcgagtt gctcgccatg
    16741 ctcgaaggcg ccacggtgcc cgtgccgatg gactacggct tctcggccta cgggcagcgc
    16801 gtggaactct cgacgcgcaa catcccgttt atcgcctgcg gggccttctc cggcctcgac
    16861 gagatgctca aggcgcggcg gggcaacatc ggcttccgtg gcgggggcga cggggaagac
    16921 ccgacgacgt tgacgctcga cgaggtcgag tcgttccaga agtacggctt cctgcccgaa
    16981 ctcatcgggc gcttctcgcg catcgtcgtc tttcagccgc tcccggccga gacgctgcgg
    17041 cgcatcctct cggagaacgt gttgccgcag ttcgagggcg agttccgcgc cgagggcttg
    17101 cggctcaccg tgacggagga cgcgctcgcg cacatcgtcg cgcgcagcca gcgacgcggc
    17161 acgggcgcgc gcggcctcaa cacggagctg atcgcggcgg tcgagcgcgc cgccttcgag
    17221 accttcgcgc gcaccaccaa cgccgaggtc atcatcacgc tcgcggacgg ccggctggaa
    17281 agcgaagttc gcaagcttgc gtaaaggccg tcaatcgtca atcgttaatc gaagttgagc
    17341 atcgctcgac ttccgaggtt tatcttaagc ggcaagagcg tcaatcacga tttacgattt
    17401 acgattcacg attcacggcc tttctttatg aacagtctca gccttcactc ctcgtccgtc
    17461 aaagaagccg tcgatttaga atcggaggtc gcgcgccttg aagccgcgct cgccgggcgg
    17521 cgcgccgaac tcacggcctt gcaggatggc ctgcgtgagt tcaagacgcg ttacgcgcag
    17581 gtcgtcggca gtcgtctggc ggagctggcg gaggtggaga aggcgatcag ggaggccgag
    17641 gggcgtgtgc tcggcgtcga gcgtgagccg gaagaggaag aggccgagac agacttctac
    17701 ggcggacaag atgcgagcgc ggcgccggtg ggcaagtctc tgcggaggct gttctggtcg
    17761 gtggcgaaga tgtttcaccc cgaccacgcg gccgacgagg gcgaggcccg acgccgccac
    17821 acggtgatgg ccgaggcgag ccgcgcctac cgcgagggcg acgtggagag tttgcacaca
    17881 ctgctcggcg acgagggcct gcaatccttc tgcacgggcg cgcagccgga cgaagacccg
    17941 gaagatttgg gcgcgcgcgc cctccggctt gagctggagc tgcgaacaat cgagtttggc
    18001 atcaagcgca ttcggctcga cgcgctgtat cgtctgaagc tggagatcga agcggaggcc
    18061 gcgcgcgggc gcgacgcgct cacgctctcg gccgagcgca tcgaccgcca gatcgtcaag
    18121 gcgcgccgcc ggctcgaaaa cctctcgtaa agatcaggaa ggcgttaaca gcgtgtctaa
    18181 aaagtcccgt ctgcgccgcg gagcggcagc cagaaggtag ccagacgtga aacgtctggg
    18241 acacgcaacc acgatgtccc cgcgctgaag gcgcggcaga gcgtctctgc gcgccccttc
    18301 agggcgcgtg cgtgattcgg gcgccggccc agacgtttca cgtctggcta ccttctcggc
    18361 acgcctccgg cgtgtcacag actttttaag atacactcta accacgacga gtatgtacac
    18421 cagcgacacg cccccggccc gaaagcgtct gatcaagtat ctcgtcttca agtcagtgat
    18481 cgacctgctg ttcgtcggcg gcctcgccgt cggcttctat ctcgacgcct tcaacccatc
    18541 cctgcgcggc tcgctcgacg aggcgggggc gcgaggggtc gccggatggg tggtcgataa
    18601 gtcgaacccc gcggcgcacg tcgaggtgca gctctacgtg aacggccgct ttgtcgagag
    18661 ccggcaggcc gacttcgggc gggccgacct cgtggcggcg ggtgtggctg aag
//
LOCUS       DASVES010000150         7462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1128678, whole genome shotgun sequence.
ACCESSION   DASVES010000150 DASVES010000000
VERSION     DASVES010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128678"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VF240_05840"
     CDS             complement(<1..380)
                     /locus_tag="VF240_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7174786.1"
                     /translation="MSKQPGAGRRAPIKHLRWWIGGLLFASTVINYIDRQTLSNLAPF
                     LKKDYGWTNTDYAYLVISFRIAYSIGQTVCGRLVDKLGTRRGLTLSVVWYSVVSMLTS
                     LAGGFYSFAAFRFLLGVGESANWPA"
     gene            complement(420..2960)
                     /locus_tag="VF240_05845"
     CDS             complement(420..2960)
                     /locus_tag="VF240_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEX7174787.1"
                     /translation="MLVSNLHKLVSGIAARPRAESGAYEPHGGRQSLLASLFEGGARG
                     LSTRAAGLLSLLRQTYGDDAGAAEKNLNRLGALCASFLEAYGEGPVRLLRAPARINIL
                     GEHVDYVSYLPTYSLPFGSREHDMLMMYGPSATGRVRGASRRGEFARFDFSLDEGPAP
                     GGGVGYEAEWLSYVFKGETPAPHWGNYVKGSCFFARTKFGGRVARGFDFVVDSSIPPK
                     GGASSSSALAVLAGAAIREVNGVAYEPGELAVDSSKAEWFVGTRGGSMDHLTICLARR
                     GRAVRISYREGTTRALQLPSMRLRWVTFFSHPADKGREIMLEYNERAAVSRLLIPAII
                     EGWEDTDPRRHAAWGRAVEALLSGGEGAADEAESLLRELPETITLEEVERDYARAYGE
                     CAEAFPALVEGRRGRPLRVRAYALHHLGEVRRVLAAERLLDSPSESDGRQALSRLGEL
                     LNETHASLRGFYGVSTPEVERLVGIILSDPGVRGARLMGGGFGGNVLALTTEENVSVL
                     IRRVQAEFYASAGRDGEREGSVMVSTPGDGLSGLGLESVWRRAVEEFNALGRAGAVYR
                     RGIASMLDDVEADTVRDEVWPIIVAAGKGSRARESGLDVPKPVASVAGVPAILRVLRN
                     VMEGCGGLAHAPIVIVSPETERAVREALAGEEVSYVMQRVSLGTGDAVLQARELMKDF
                     DGRALVIWGTQPAVRPRTVARALRLAALFDVYDMVLPTALRERPYAPVERDEQGHVLA
                     SRETHLEGVPRPEFGETNNGLFLLKSRVMFDTLVELRRRHWHESEGRYDRTGELGLPN
                     EMINFLAARAGGVLACPFTDAREGQGIKTLEDVALCERFISELDGAEA"
     gene            3438..4298
                     /locus_tag="VF240_05850"
     CDS             3438..4298
                     /locus_tag="VF240_05850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174788.1"
                     /translation="MNPNAEKFGIRLLEESDVAEAMRLKAARWNQTEEDWLRLLRLCP
                     RGCFAATAGVRLVGTTTITVYGRGLAWVGMVLVDPDFRRRGIATALVREALKGLDAAA
                     VTTVKLDATPEGAPVYEALGFEAELHIERWVGVVQAKASPAHTAEGAPQPSQILELDR
                     RAFGADRSELLRALLEESCVTPAVSMGADGRLRGYALARRGAMADYVGPIVAEEAETA
                     AALLDDVLNRLDAGRVCVDLNTTYAGGARVLEAQGFVKQRELIRMRRGGRSGAGTSDS
                     IFAIAGPEVG"
     gene            4329..5135
                     /locus_tag="VF240_05855"
     CDS             4329..5135
                     /locus_tag="VF240_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX7174789.1"
                     /translation="MESRVQRGAAEDGLVERFQAGRLNVYVYESRRMMGAVAAAAVSA
                     EIRRLQGERGRAVVIFASAPSQNEFLAELAGVPGVDWPKVKGFHLDEYLGMEDSAPQS
                     FRRFLLDRLVNKVDFGEFHGLRGEAEDAAAECRRYAALLAENPPDFAVLGIGENGHLA
                     FIDPPFCDFDDPEPVKVAELDEVCRVQQVHDGAFAALEDVPRDALSLTIPTIMSRPKL
                     FAIVPGPAKAQAIKGVIEGPIETACPASILRNHPDAHLFIDRDSASLLSR"
     gene            5188..6552
                     /locus_tag="VF240_05860"
     CDS             5188..6552
                     /locus_tag="VF240_05860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7174790.1"
                     /translation="MRLAKSGRAFLGLTLACFVTFLLAPPAYAEGGAGGDGRRGEASR
                     FEQLVEANVIATHAFQILLAKKSGKGDAACYAQGGLSDVELETLVKHQSSLLKSKAGD
                     VLAWARGGRSGFDSSKDLEPILKSGLAVPPGSPANVFTDYLRAHTRSGADVARLRSVA
                     SLYQTILEVERDGDLLQDQFAFYIALGLPVYVGQLSLPGGDDAMLAVGRKLERRACAS
                     PYAADAAAWQIAGRKVWDWGEKHLHIRDDKVLAAELLRERDVRAVVPRVRAVPAQKIA
                     VVGHSFTMGQHWSSPSSFVPVVTEIFRRVNPKVEFKQFERGGLTASRARQRFYQGVLD
                     WKPDKVLLVVMTRTDEDYAALKEMGAGFKAAGVEAFMFDDIHDPESVSKPGTSARAAR
                     VAREVGIKVIEVGALLAAAPDRGRFLSLDRVHMTEPYHRLMAKEWLKFLAGVRGERLA
                     GRAE"
     gene            6573..>7462
                     /locus_tag="VF240_05865"
     CDS             6573..>7462
                     /locus_tag="VF240_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174791.1"
                     /translation="MKVNREQKNSKGGVLRGALVALTLSSSFLFLGEAASSGGARAQM
                     SPGQTRQASAVVRPRMLVGERDPLTGFQTLRARYDAGARPPDDVAGWALTYLLKGDDA
                     FARRAVEEMRRARTPERVGSRTYPEFVKWSLAFDWLYNYPGFDVALKDRVAGELVKAA
                     AQMLQDQSLRDPQLSMYHNYTVRYLTLAAFALTAVEGHPSVEAEAAPLRERTRRVWEH
                     ILDLTDFITPDGGYHESMDYQRITYAPMALLAELDRTALNADPALRYTVFRHYAATHL
                     YKALPDGTTSRLGDNEFPYMR"
BASE COUNT         1262 a         2528 c         2510 g         1162 t
ORIGIN      
        1 gccggccagt tggccgactc gcccacgccg agcaggaagc ggaaggccgc gaagctgtag
       61 aagccgccgg ccagcgacgt gagcatcgag acgacggagt accagacgac cgagagcgtc
      121 agcccccgcc gcgtgcccag cttatcaacg aggcggccgc agaccgtctg cccgatggag
      181 taggcgatgc ggaaggagat gacgaggtag gcgtagtcgg tgttcgtcca gccgtagtcc
      241 ttcttgagga agggggcgag gttcgagagc gtctggcggt caatgtagtt gatgacggtc
      301 gaggcgaaga gcaggccgcc gatccaccac cgcaggtgtt tgatcggcgc gcggcgtccc
      361 gcgccaggtt gtttagacac tgccattcgt tcggctcatc ctttagttcg ggttcgtagc
      421 taagcctcgg caccatccag ctcggagatg aagcgctcgc acagcgccac gtcctccaga
      481 gtcttaatgc cttgcccctc acgggcgtcg gtgaaggggc aggccagcac tccgccggcg
      541 cgcgccgcca ggaagttaat catctcgttc ggtaagccaa gttcgcccgt ccggtcgtaa
      601 cgcccttcgg actcgtgcca gtgccggcgc cgcaactcca ccagcgtgtc gaacatcacg
      661 cggctcttga gcaggaagag gccgttgttc gtctcgccga actcggggcg cggcacgccc
      721 tccaggtgcg tctcgcgcga cgcaagcacg tgcccctgct cgtcgcgctc gaccggcgcg
      781 taaggacgct ccctgagcgc cgtcggcagc accatgtcgt acacgtcgaa gagcgcggcg
      841 agccggagag cgcgcgcgac cgtccgcggc ctgacggcgg gctgcgtgcc ccagatgacc
      901 agtgcgcgcc cgtcgaaatc cttcattaac tcgcgcgctt gcaggacggc gtcgcccgtg
      961 ccgagcgaca cgcgctgcat gacgtagctc acctcctcgc cggccagcgc ctcgcgcacc
     1021 gcccgctcgg tctcgggcga gacgatcacg atgggcgcat gcgccaagcc gccgcagccc
     1081 tccatgacgt tacgcaacac gcggagtatg gccggcacgc cggctaccga cgccactggc
     1141 ttcggcacat ccagccccga ctcgcgcgcc ctcgacccct tcccggccgc gacgattatg
     1201 ggccacactt cgtcccgcac ggtgtccgcc tctacgtcgt caagcatcga ggcgatgccc
     1261 cgcctgtaga cggcccccgc gcgccctagc gcgttgaact cttcgaccgc ccggcgccag
     1321 accgattcga gccccagccc cgagagcccg tcgcccggcg tggagaccat caccgagcct
     1381 tcgcgctcgc cgtcccggcc ggcggaggcg taaaactccg cctgcaccct gcggatgagc
     1441 acggacacat tctcctccgt cgtcagcgcg aggacattgc ctccgaaccc gccgcccatc
     1501 agccgcgcgc cgcggacgcc agggtcggag aggatgatgc cgaccagcct ctccacctcc
     1561 ggcgtactca cgccgtagaa gccccgcagg ctcgcgtgtg tctcgttgag cagttcgccc
     1621 agacgtgaca gcgcctgacg cccgtccgat tctgaaggag agtcgaggag gcgctcggcc
     1681 gccaagacgc ggcggacttc gccgaggtgg tgcagggcgt aagcgcgcac gcgcagcggc
     1741 cgcccgcgcc gcccttcgac gagcgccggg aaggcttcag cgcactcgcc gtacgcacgt
     1801 gcgtaatccc gctcgacctc ctccagcgtg atcgtctcag ggagttcgcg gagcagcgac
     1861 tccgcctcgt cggccgcgcc ctccccgccc gagagcaacg cctcgacggc gcgaccccac
     1921 gccgcgtgac gccgcgggtc tgtgtcctcc cagccttcta tgatcgcggg tatcagcagg
     1981 cgcgagacgg ccgcgcgctc gttgtactcc agcatgatct cccgcccctt gtccgccggg
     2041 tgggagaaga aggtgaccca gcgcagccgc atcgacggga gctgtagggc gcgcgtcgtc
     2101 ccctccctgt aagagatgcg cacggcgcgc ccgcgcctcg cgaggcagat cgtcaggtga
     2161 tccatcgacc cgccgcgcgt gccgacaaac cactcggcct tcgacgaatc cacggccaac
     2221 tcgccgggct cgtaagccac gccgttgacc tcgcgtatcg ccgcgccggc gagcactgcc
     2281 agcgccgaag aggacgaagc cccgccctta ggcgggatgc tcgaatcgac gacgaagtcg
     2341 aagccccgcg ccacccgccc gccgaacttc gtgcgtgcga agaaacacga acccttaacg
     2401 tagttgcccc agtggggcgc cggcgtctcg cccttgaaga cgtaagagag ccactccgcc
     2461 tcgtaaccca ctccgcctcc gggcgcgggg ccttcgtcga gcgagaagtc gaagcgcgcg
     2521 aactccccgc gacgtgaggc gccccggacg cgccccgtcg ccgagggccc gtacatcatg
     2581 agcatgtcgt gttcgcggct gccgaagggg agtgagtacg tcggcaggta agacacgtag
     2641 tcgacgtgct cgccgaggat gttgatgcgc gccggggcgc ggagcaggcg gactggtcct
     2701 tcgccgtagg cctccagaaa gcttgcgcac agcgcaccca gcctgttgag gtttttctcg
     2761 gcggcccccg cgtcgtcccc gtaagtctgc cggagaagtg agagcagccc cgccgcccgc
     2821 gtcgacagtc cccgggcgcc gccttcaaag agtgacgcga gcagcgactg ccgaccgcca
     2881 tgcggttcgt acgcccccga ctcggcacgg gggcgggcgg cgatgccgga tacgagcttg
     2941 tgtaggttag agactagcac cgttgtgact aaagagggct gaacggccct atcgactcta
     3001 aagcgttttc gataaagaga acggcggttg ctgcgtcggg gtttgttcga gactctgaca
     3061 aatgcggtca ccgtagaccg gcggagcgcc gtttaagaca tctctcggcg ccccggagac
     3121 gtgcttatac catcacacgc cctctaagcc aaaggcccac ggtgcctcgg gacggcgcac
     3181 acggtgaaga cgtgcggagg cgagaggcgg ggcagcaaag tttcataaaa gcaattctcc
     3241 caaggtatag atattcggcc cggcggccta atccttgcgg ccgcgctcgg gttagtgcta
     3301 taaaatcgtt cccgacaacg cgtcgtgttc tgaaattctg tagagcgatc cgcccacaat
     3361 tcgtgtcacc ccgaagtctt tcggaaactg atgggcgaaa gtattcattg aggtttgtca
     3421 ggaaagtaaa caaactcttg aacccgaacg ccgagaagtt tggaattcgt ctgctcgaag
     3481 agagcgatgt cgctgaggcg atgagactca aggcggccag atggaatcag acagaagagg
     3541 actggctcag actcctcagg ctctgcccgc gcggctgttt cgctgccacc gccggcgtga
     3601 gactcgtcgg gacgacgacc atcaccgtgt acgggcgggg actcgcctgg gtaggcatgg
     3661 tactcgtcga ccccgatttc cgccggaggg gcatcgcgac ggcgctcgtc cgtgaggcgc
     3721 tcaaaggcct tgacgcggcc gcggtgacga cggtgaaact ggacgcgacg cccgagggcg
     3781 cccccgtcta cgaagcgctg ggcttcgagg ccgagcttca catcgagcgc tgggtgggcg
     3841 tagtccaggc caaagcctcg ccggcacata cggcggaagg cgcgccccaa ccgtcgcaaa
     3901 tcctcgaact cgaccggcgc gcgttcggcg ccgaccgctc ggaactgctg agggcgcttt
     3961 tggaagagtc gtgcgtaacg ccggcggtga gcatgggggc ggacgggcgg ctgcgcggct
     4021 acgccctggc gaggcgcggg gcgatggctg actacgtcgg gcctatagtg gcggaggagg
     4081 cagagacggc cgccgccctc ctcgacgacg tgctcaatcg tctggatgcg gggcgggtct
     4141 gcgtggactt aaacacgact tacgccggcg gcgcgcgcgt gctggaggcg cagggcttcg
     4201 tcaaacagcg cgagctgatc cgcatgcgcc gcggtgggag aagcggcgca ggcacttccg
     4261 acagcatctt cgccatcgcc gggccggaag tgggttgagg cgcgaagtct tccatctgag
     4321 gagtgaacat ggaatcacgg gtgcagaggg gcgcggccga ggatggtctc gtcgagcggt
     4381 ttcaggcggg gcgcctgaac gtgtacgtct acgagtcgcg gcggatgatg ggggcggtcg
     4441 cggccgccgc cgtgtcggcg gagattcgtc gcctgcaagg tgagcggggc cgcgctgtcg
     4501 tcatcttcgc ctccgcccct tcgcagaacg agttcctggc ggaactcgcc ggagtcccgg
     4561 gcgtcgactg gcctaaagtc aagggctttc acctcgacga gtacctgggg atggaggaca
     4621 gcgcgccgca gtccttccgc cgcttcctgc tcgaccgcct ggtgaacaag gtggacttcg
     4681 gcgagttcca cgggctgcgc ggcgaggccg aggacgccgc ggctgagtgc cgcaggtacg
     4741 cggcgctgct cgcggagaac ccgcccgact tcgccgtgct cggcatcggc gagaacgggc
     4801 atctggcctt catcgacccg cccttctgcg acttcgacga cccggagccg gtcaaggtgg
     4861 ccgagctgga cgaggtctgc cgcgtgcagc aggtgcatga cggcgccttc gcggcgctcg
     4921 aagatgtccc gcgcgacgcg ctctccctga ccatcccgac catcatgtcg aggccgaagc
     4981 tgttcgccat cgtgcctggg ccggcgaagg cgcaggccat caagggcgtc atcgaggggc
     5041 cgattgagac ggcgtgcccg gcctccattc tcaggaacca cccggacgcg cacctcttca
     5101 ttgaccgcga ctcggcctcg ctcctctcgc gctgaaggcg cgggcagtcc ggccgaagcc
     5161 gcaaagcttt aaaggttgag agcggagatg agacttgcaa agagcggacg cgcgttcctg
     5221 gggttgacac tagcctgctt cgttaccttc cttttggcgc cgcctgccta cgccgagggc
     5281 ggagctggcg gtgacggtcg gcggggcgag gcgtcgaggt tcgagcaact cgtcgaggcg
     5341 aacgtcatcg ccacgcacgc tttccaaatt ctgctcgcca aaaagtccgg caagggggac
     5401 gcggcgtgtt atgcgcaggg cggcctgtcg gacgtcgagt tggagacgct cgtcaagcac
     5461 cagagcagcc tcctgaagag taaggccggg gatgtcctgg cctgggcgcg cggcggccgg
     5521 tcgggcttcg actcgtcgaa agacctcgaa ccgatactga agtcggggct ggccgttccc
     5581 cccggctcac cggccaacgt cttcaccgac tacctgcgcg cacacacccg ttccggggcg
     5641 gacgtggctc ggctgcgctc ggtcgccagc ctctaccaga ccatcctcga agtggagcgg
     5701 gacggcgacc tccttcagga tcagttcgcc ttctacatag cgctcggcct ccccgtctac
     5761 gtcggccagt taagtctgcc gggtggcgac gacgcgatgc tcgccgtggg gcgcaagctg
     5821 gagcggcgcg cctgcgcctc gccctacgcg gcggacgccg ccgcgtggca gatcgcgggg
     5881 cgcaaggtct gggactgggg cgagaagcac ctgcacatac gcgacgacaa ggtactcgcc
     5941 gcggagcttc tacgggagcg cgacgtgcgc gcggtcgtgc cgcgcgtccg tgccgtgccc
     6001 gcgcagaaga tagccgtggt cgggcactcg ttcacgatgg ggcagcactg gtcttcgcct
     6061 tcgagcttcg tccccgtcgt gaccgaaatc ttccggcgcg tgaacccgaa ggtcgagttc
     6121 aaacagttcg agcggggcgg cctgacggcc tcgcgcgcca gacagcgctt ttatcagggc
     6181 gtgctcgact ggaagccgga caaagttctg ctcgtcgtca tgacgcgcac cgacgaggac
     6241 tacgcggcgc tcaaagagat gggcgcgggc ttcaaggctg cgggcgtcga ggctttcatg
     6301 ttcgacgaca tacacgaccc ggagagcgtc tcgaagccgg ggacgagcgc gcgcgccgcc
     6361 cgcgtcgcgc gcgaggtggg catcaaggtc atcgaggtcg gcgcgctgct cgcggccgcg
     6421 ccggaccgcg gacgcttctt gagcctcgac cgcgtacaca tgaccgaacc ctaccaccgc
     6481 ctgatggcga aggagtggct caagttcctg gccggcgtgc gcggcgagcg gctcgcgggc
     6541 cgcgccgaat gaccgggttg agaaggcaga agatgaaggt taacagggaa cagaaaaact
     6601 cgaagggagg tgtgttgcgc ggggcactcg tcgcgctcac gttgtcctcc tccttcctct
     6661 ttttggggga ggcggccagc tcagggggcg cgcgtgcgca gatgtcgccg ggacagacgc
     6721 ggcaggcgtc cgcggtcgtc aggccgcgca tgctcgtcgg agagcgcgat ccgctgacgg
     6781 gcttccagac cctgcgcgct aggtacgacg cgggcgcgcg accgccggac gacgtggccg
     6841 gctgggcact cacatacctg ctcaagggcg acgacgcgtt cgcgcgccgc gccgtcgagg
     6901 agatgcggcg cgcgcgcacg ccagagcgcg tcggctcgcg cacctacccc gagttcgtca
     6961 agtggtcgct cgccttcgac tggctctaca actacccggg cttcgacgtg gcgctcaagg
     7021 accgcgtggc cggcgagctg gtcaaggccg ccgcgcagat gctccaggat cagtcgctcc
     7081 gcgacccgca gctctcgatg taccacaact acaccgtcag gtatctcacg ctcgcggcct
     7141 tcgcgctgac ggcggtcgag gggcacccgt cggtcgaggc cgaggccgcg ccgctgcgcg
     7201 agcgcacgcg acgcgtgtgg gagcacatcc tcgacctgac cgacttcatc acgcccgacg
     7261 gcggctacca cgagtcgatg gactaccagc gcatcaccta cgccccgatg gcgctgctgg
     7321 cagagctcga ccgcaccgcc ttgaatgcag accccgcgct gcgctacacg gtcttccgcc
     7381 actacgcggc cacgcacctc tacaaggctc tgcccgacgg cacgacctcg cgcctcggcg
     7441 acaacgagtt cccgtacatg cg
//
LOCUS       DASVES010000151         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_230384, whole genome shotgun sequence.
ACCESSION   DASVES010000151 DASVES010000000
VERSION     DASVES010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230384"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VF240_05870"
     CDS             complement(<1..928)
                     /locus_tag="VF240_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174792.1"
                     /translation="MSANPLQTNAVGMGRRTSQPPTRHDFSKIRTAVRIPNLIEIQRE
                     SYNRFLQMDMLPEEREPTGLQAVFRSVFPISDFRATADLDFVEYHIGNWQCKCGRLEG
                     LRYLRANCKECGALVKVDPLTPGETLCHSCGTFNAVRPTLCDNCGEPVGLKHKHDQQE
                     CQERGMTYSVPLKVKIRLTVYDKDAETGTKSIRDIKEEEVFFGEIPLMTDTGTFIING
                     TERVIVSQLHRSPGVFFEKQPVYLAKVIPYRGSWVEFEYDQKNLLYVRIDRKRKFLAS
                     IFLRALGIWLNPNFHPKGFASDSLLEEAVKNAS"
     gene            complement(1310..1693)
                     /gene="rplL"
                     /locus_tag="VF240_05875"
     CDS             complement(1310..1693)
                     /gene="rplL"
                     /locus_tag="VF240_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7174793.1"
                     /translation="MAATREDALEYIKSMTLLEASEFVKLLEETFGVSAAAATVAAAP
                     AAAGGDAAPAAEEQTEFDVILTAAGGQKINVIKVVREITALGLKEAKDLVDGAPKAVK
                     EGVSKEEAAQIEDKLKAAGASVEVK"
     gene            complement(1951..2490)
                     /gene="rplJ"
                     /locus_tag="VF240_05880"
     CDS             complement(1951..2490)
                     /gene="rplJ"
                     /locus_tag="VF240_05880"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7174794.1"
                     /translation="MKTREQKQKDLDALSRQFQEATAGMFVGFKKMSVAKDQELRRRL
                     AEAGARYSVVKNTLARKAAAGTPFEQAAEHFKGVTAMAMSTSDPVVLSKAVSKFAKEN
                     PDVFTFKVGVVEGRAVALKDVESIASLPSKEELISKVMFLINCQAQRLVTVLSAVPRN
                     LAIVIKQIGEQKGEGGETA"
     gene            complement(2509..3213)
                     /gene="rplA"
                     /locus_tag="VF240_05885"
     CDS             complement(2509..3213)
                     /gene="rplA"
                     /locus_tag="VF240_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7174795.1"
                     /translation="MAAHGKKYRAALEKIEFGRKYTLEEGLSKVREVSAVTARKFDET
                     VELTMWLGVDPRKADQLVRGTVVLPHGLGKSKRVLVIAQGEKMKEAEEAGADFIGGED
                     MVEKIKGGWLDFDAVIATPDMMRLVGALGKVLGPRGLMPNPKTGTVTFDVRNAVSETK
                     AGKVEYRVDKTGVIHVGIGKVSFEPDKLRENAQTLLDAVVKAKPSTAKGKYVKKVNLA
                     PTMGPGVLLDETVYAK"
BASE COUNT          575 a         1055 c          976 g          686 t
ORIGIN      
        1 acgacgcgtt cttgacggcc tcttccagaa gcgagtcgga ggcgaagccc ttcgggtgga
       61 aattcgggtt gagccagatg cccagcgcgc gcaggaagat cgacgcgagg aacttccgct
      121 tgcggtcgat gcggacgtac aggaggttct tctggtcgta ctcgaactcg acccacgagc
      181 cgcggtacgg gatgaccttc gccagataca cgggctgctt ctcgaagaag acgccgggcg
      241 agcggtggag ctgcgagacg atgacgcgct ccgtgccgtt gatgatgaag gtgccggtgt
      301 cggtcatcag cgggatttcc ccgaagaaga cctcctcctc cttgatgtcg cggatcgact
      361 tcgtgccggt ctcggcgtcc ttgtcgtaga ccgtgaggcg aatcttcacc ttgagcggca
      421 cgctgtaggt catgccgcgc tcctgacact cctgctggtc gtgcttgtgc ttgaggccga
      481 cgggttcacc gcagttgtcg cagagcgtgg gccgcacggc gttgaaggtg ccgcaactgt
      541 ggcacaaggt ctctcccggc gtcagcggat cgaccttgac gagcgcgccg cactccttgc
      601 agttggcgcg caggtagcgc aagccttcaa gccggccgca cttacactgc cagttgccga
      661 tgtgatactc gacgaagtcg aggtcggccg tcgcgcggaa gtccgagatc gggaagaccg
      721 agcggaagac ggcctgaagg cccgtcggct cgcgctcctc gggcagcatg tccatctgga
      781 ggaagcggtt gtaggactcg cgctgaatct cgatcaggtt cgggatacgc acggccgtcc
      841 tgatcttcga gaagtcgtgg cgcgtgggcg gctgactggt gcgtcggccc atcccgacgg
      901 cgttcgtttg gagcggattt gcagacatct ttcaagtacc ccaagtgata agtgatgagt
      961 gataagtgat aagaaaatca gtcttcctct tatcacttat cactcatcac ttatcacggt
     1021 tttcaaataa tgacgcgaaa gcgcggccgg agcgcacgat gccccgaccg ctttgagcga
     1081 ctcaaaacag ttagttgtaa tgcccaaatt gtgggagtaa cgccagcgcc tcaatactcc
     1141 ccaccttagc cgcccggcgg ccccgctgtc aagacgataa gacggtgatg agtgacaagt
     1201 gatgagtgat aagaacagaa gctgttagca cttagctgtt ggctgttagc taatcgctaa
     1261 gagctaacag cttcttatca cttatcactc atcactcatc acgacattgc tacttgactt
     1321 cgacggatgc tccggcggcc ttgagcttat cctcgatctg agcggcctct tccttggaga
     1381 cgccctcctt gacggccttc ggagcgccgt caacgaggtc tttggcctcc ttcaggccga
     1441 gggccgtgat ctcgcggacg accttgatga cgttgatctt ctgaccgccg gcggccgtca
     1501 ggatcacgtc aaactcggtc tgctcctcgg ccgcaggggc cgcgtcgccg cccgccgccg
     1561 ccggggccgc cgccacggtt gccgccgccg ccgagacgcc gaaggtctct tcgagaagct
     1621 tgacgaactc cgaagcctcc agcagggtca tgctcttgat atattccagt gcgtcttcac
     1681 gtgttgctgc catgattacc tctcatcctt ccccgaaggg ttctgttgtt gctgtggttg
     1741 acgtttcgca gcacgagaac ccacgggacg cttccgttca cccgctgcat cgcgagcctc
     1801 aaaaagcgtc cgagccaaat cgggccgacc tgacaatcgc cccgctcgtt ttccgcgtcc
     1861 ggctgacttt caatcaaatc ccaaagtcga gatttgaaat ttcaaacccg aagggggcaa
     1921 agcgcgcgcc gccccgaaac tttttctcta ttaagcggtc tcgccgcctt cgcccttctg
     1981 ctcgccgatc tgcttgatga cgatggcgag gttgcgcggc acggccgaga gcacggtgac
     2041 gagccgctgc gcctgacagt tgatgaggaa catgaccttc gagatcaact cctccttcga
     2101 cggcaggctg gcaatcgact ccacgtcctt aagcgccacg gcccggccct caacgacgcc
     2161 caccttgaag gtgaacacgt cggggttctc cttggcgaac ttcgagacgg ccttcgagag
     2221 gacgaccggg tcgctcgtgc tcatggccat cgccgtgacg cccttgaagt gctcggcggc
     2281 ctgctcgaac ggcgtgccgg cggcggcctt gcgggccagc gtgttcttga cgaccgagta
     2341 gcgcgcgccc gcctcggcga ggcggcggcg cagctcctga tccttggcca cgctcatctt
     2401 cttaaacccg acgaacatcc cggcggtcgc ttcctgaaac tgccgcgaga gggcgtcgag
     2461 gtctttctgc ttctgctccc tggtcttcat aactttctcc cgtcctccct acttcgcgta
     2521 cacagtctca tcgagcagca cgcccggccc catcgtcgga gcgaggttga ccttcttgac
     2581 gtacttgccc ttggcggtcg agggcttggc cttgacgacc gcgtcgagca gggtctgcgc
     2641 gttctcgcgc agcttgtccg gctcgaacga caccttgccg atgccgacgt ggatgactcc
     2701 cgtcttgtcc acgcgatact cgaccttgcc ggccttcgtc tcgctcacgg cgttacgcac
     2761 gtcgaacgtc accgtccccg tcttcgggtt gggcatcagg ccgcgcgggc caagcacctt
     2821 accgagcgcg ccgacgaggc gcatcatgtc gggcgtggcg atgacggcgt cgaagtcgag
     2881 ccagccgccc ttgatctttt cgaccatgtc ctcgccgccg atgaagtcgg ccccggcctc
     2941 ttcggcctcc ttcatcttct cgccctgcgc gatgacgagc acgcgcttcg acttgccgag
     3001 gccgtgcggc agtacgaccg tgccacgcac caactggtcg gccttgcgcg gatcgacgcc
     3061 gagccacatg gtgagttcga ccgtctcgtc gaacttccgc gccgtgacgg cggagacttc
     3121 cctgaccttc gacagaccct cttcgagtgt gtacttccgc ccgaactcga tcttctcaag
     3181 cgccgcgcgg tacttcttgc cgtgtgctgc catttaactt cctccgaggt gcaagcggag
     3241 cggacgacgg ccgcgcctcc ctcttcaaga gggatgaggg cggagggatg ag
//
LOCUS       DASVES010000152         7432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_609743, whole genome shotgun sequence.
ACCESSION   DASVES010000152 DASVES010000000
VERSION     DASVES010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609743"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="VF240_05890"
     CDS             <1..1884
                     /locus_tag="VF240_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7174796.1"
                     /translation="PGHGHDDFVIGKSYGLEIYCPVDSAGRFTSEVEHFAGMSVFEAN
                     PRIVEFLRESGALLFSEDYRHRYPHCWRCKNPVIFRATPQWFVSLDAPSSEAQSVETD
                     EDGRDLTNYTDNETDVPQSLRQGALREIATVDWVPAWGEGRMRNMLTNRPDWCVSRQR
                     VWGVPIPAFYCAGCDAAVADAEVIRHVADIFERESADAWYARDPRDLLPEGFKCRKCE
                     GAEFTKETDILDVWFDSGSSSVAVLESYEGLRWPADVYLEGGDQYRGWFNSSLIVGLA
                     AHDHAPFRTVITHGWVVDVDLQKMSKSQGNGISPHEVIKESGADILRLWVASSDYQED
                     VRCSDEIMLRAVDTYRKMRNTARFALGNLAGFDPARDSVAEDELHETDRWALAELDAV
                     TARAREGYETYAFHAAYQALYHFCTVTLSARYFDIIKDRLYTAAPRSPARRSAQTALY
                     RIADALARLLAPILVYTADEIWENLPADESRPVSVHLAEFPEKCETRDDELMERWTRL
                     FEVRDMVLPKLEEARTAKLIGSSLEARVVLSAGESPELRDLLERYSDELRYLFIVSEV
                     ELEEPTAGELLSVRVERARGEKCERCWNYSVRVGESERYPTACERCVEALREIEREEA
                     AGS"
     gene            1881..2417
                     /gene="lspA"
                     /locus_tag="VF240_05895"
     CDS             1881..2417
                     /gene="lspA"
                     /locus_tag="VF240_05895"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7174797.1"
                     /translation="MSEARKIGWRGAYLLAAAGVYMADQASKAWAARRLRFGDTVTVV
                     ERLLYFDYATNDGIAFGQLQEGGEFSRWMLVTLAALAAVAVLVYFFRTPRAEDRVLGA
                     CALLLAGIAGNLTDRVRLGHVIDFVEVHLGSFQWPTFNVADAAICVGAGLLALDIILE
                     GKGVRGKGERKEAEGTAS"
     gene            2469..3371
                     /gene="lgt"
                     /locus_tag="VF240_05900"
     CDS             2469..3371
                     /gene="lgt"
                     /locus_tag="VF240_05900"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7174798.1"
                     /translation="MYPELFRIGGFPVNTYGVLLALAFLVALYVASRLGERDGLRRER
                     IYDLGLWMLLAGLVGSKLLMMLVEPEYAENPARLLSLDFLRSGGVWYGGFIGGVTAGY
                     FLMRRWKLPFWKTADSFVPGIALAQAIGRQGCFAAGCCWGEPTLRAWGVHFSEHGHSV
                     TGVPTTVAQLGASERAVWAERLGGFDAPVYLHPTQLYESFAMLAAFVFLLWLHRRKRF
                     SGQVLLAYAALYGAVRFTIEFFRADPRGDIAGLTSLTGLSTSQIISLLIGLTGVILLV
                     VRRRRAPSAPESDGEAAVTNTASA"
     gene            3599..4591
                     /locus_tag="VF240_05905"
     CDS             3599..4591
                     /locus_tag="VF240_05905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7174799.1"
                     /translation="MSEEQLIGPEAETLAFHASAEEAGERLDAFLAARIATQSRSAIK
                     RLVDDGDVLVDGRVVKPSYKLRGGEEIEVETPPPPPLELAGEDIPLDIVHEDDDIVVV
                     NKPAGMVVHPAAGVPSGTLANALVYHFQQLPTRGGEARPGIVHRLDRDTSGLIVVAKT
                     PQAHEHLSEQFRVREVHKSYVALVHGRVEGERGRIEESLARDPRHRTRMAVRRGGRAA
                     LTLWRVRRRYGRFTLLDVEIKTGRTHQIRVHAAWMKHPVVGDETYNDGRDRSMSDPAL
                     RARIRAIGRQFLHAERLAFAHPSTGERPLFTAPLPPELSTFLDTLEAAEATTRE"
     gene            4972..6000
                     /locus_tag="VF240_05910"
     CDS             4972..6000
                     /locus_tag="VF240_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174800.1"
                     /translation="MRTAIFSFLLLTLAAGLLGGGALSLAYEPQGAPQTPAPRATPTP
                     VDDDDVVGIRIPLVRLPISVVDKKGQPVAGLSAGDFQIFEDKKAQQFELLSRAEELER
                     LPVYVGVLMDTSSSTAGKLKFEKEAALNFVHTVARLRKDRVAFLTFDHEVTLRQDFTD
                     KLDLLDKAINAVKAPGRQTSLYDAIWIFCNEKMRGVGSARRALVVITDGDDTYSRATL
                     KDAIDIAQRTETVIFAISTKGGFSGSAVPGVEAQTVKDSGDKDLVKLCEETGGRAFFT
                     GDMLALERSFTTIAKEIRSQYIATYRPSNDRYDGSYRRIEVKLASERDGLKVRAKRGY
                     LAVSDTVK"
     gene            6137..7354
                     /locus_tag="VF240_05915"
     CDS             6137..7354
                     /locus_tag="VF240_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174801.1"
                     /translation="MFRRSQIARALALLISFASAAVAGSGLLSSPSSARASQSHAQSQ
                     EAETSRSRRAGLAQEGDAAAAPSDQQQQTTQPAPRPTPTRPDEEIDDDEVLRVETDLT
                     NVLFTAVDKNKRFVINLRQEDIEVYEDGARQEIFTFQRETDRPLSLAILIDTSASQER
                     TLPEEKSAAQRFVDAVIRKAKDEVAVLSFTGETTLEQSLTGDPARVRRAIDRVEFVPP
                     SGYIGGGVIVPGGTPPISGTNQRLAGSTAIWDSIWVTSKEVLSESSDKTRRAIILLSD
                     GIDTSSQLKMAEAIDGAIRADAVIYAVGIGDNFFDGVDQGSMRKIAERTGGRAYFPRN
                     EEDLRVAFAQIQEELRSQYLVAYSPTRKAKDGTFRNVKIEVVNPELRRQNLRLTYRQG
                     YFAQTAAAPSRKP"
BASE COUNT         1252 a         2520 c         2387 g         1273 t
ORIGIN      
        1 ccggggcacg gccacgacga cttcgtcatc ggcaagtcat acggtctgga aatctattgc
       61 cccgtcgact cggccggccg cttcacttcc gaagtcgagc acttcgccgg catgagcgtg
      121 ttcgaggcca acccgcgcat cgtcgagttt ttgcgcgagt cgggcgcgtt gctcttcagc
      181 gaggattacc ggcaccgcta tccgcactgc tggcgctgca agaaccccgt catcttccgc
      241 gccacgccgc agtggttcgt ctcgctcgac gcgccctcct cggaggcgca gtccgtggag
      301 accgacgagg acgggcgcga cctgacgaac tacacggaca acgagacgga cgtgccgcag
      361 tctttacgtc agggcgcgct gcgcgagatc gccaccgtcg attgggtgcc ggcgtggggc
      421 gaggggcgga tgcggaacat gctgacgaac cgccccgact ggtgcgtctc gcgccagagg
      481 gtgtggggcg tgcccatccc cgccttctac tgcgcgggct gcgacgcggc cgtcgccgac
      541 gccgaagtca tccgccacgt cgccgacatc ttcgagcgcg agtcggccga tgcgtggtac
      601 gcgcgcgacc cgcgcgatct cctgcccgaa ggcttcaagt gccggaagtg cgagggcgcg
      661 gagttcacca aggagacgga catcctcgac gtgtggttcg acagcggctc gtcgtccgtc
      721 gcggtgctcg aaagttatga gggcttgcgc tggccggccg acgtttatct cgaaggcggc
      781 gaccagtacc gcggctggtt caactcgtcc ctgatagtgg gcctcgccgc gcacgaccac
      841 gcgcccttcc gcacggtcat cacgcacggc tgggtggtcg atgtcgattt gcagaagatg
      901 tcgaagtcgc agggcaacgg catctcgccg cacgaggtca tcaaggagtc gggcgcggac
      961 atcctgcggc tgtgggtcgc gtcgagtgat tatcaggagg acgtgcgctg ctcggacgag
     1021 atcatgctgc gcgccgtgga cacctaccgc aagatgcgta acacggcccg cttcgccctc
     1081 ggcaacctcg cgggcttcga ccccgcgcgc gactcggtgg ccgaagacga gctgcacgag
     1141 accgaccgtt gggcgctcgc cgaattggac gccgtgaccg cgcgcgcgcg tgagggctac
     1201 gagacttacg cgttccacgc ggcctatcag gcgctctacc acttctgcac cgtcacgctc
     1261 tcggcgcgct acttcgacat catcaaggat cgtctctaca cggccgcccc ccgctccccg
     1321 gcgcggcgct cggcgcagac ggcgctctac cggatagccg acgcgctcgc gcgtttgctt
     1381 gcgcccatcc tcgtctacac ggcggacgaa atctgggaga acctgccggc agacgaaagt
     1441 cggcctgtct cggttcacct cgcggagttt cctgagaagt gcgagacgcg cgacgacgaa
     1501 ttgatggagc gttggacgcg tctgttcgag gtgcgcgaca tggtgctccc gaagctcgaa
     1561 gaggcgcgca cggcgaagtt gatcgggagt tcactggagg cgcgcgtcgt tctgagcgcc
     1621 ggcgagtcgc cggagctgcg cgacctgctc gaacgctaca gtgacgagtt gcgctacctg
     1681 ttcatcgtgt cggaggtcga gcttgaagag ccgacagcgg gcgaactgtt gtcagttcgc
     1741 gtcgagcgtg cgcgcggcga gaagtgcgag cggtgctgga actactccgt gcgcgtcggc
     1801 gagtcggagc gttacccgac ggcgtgcgag cgttgcgtcg aagcactacg agaaatcgaa
     1861 cgtgaggagg cggcggggtc atgagcgagg cgcgcaagat cggctggcgc ggggcgtacc
     1921 tgctggcggc cgcgggcgtt tacatggccg accaggcgtc gaaggcgtgg gcggcgcggc
     1981 gtctgcgctt cggcgacacg gtcacggtcg tcgagcgcct gctctacttc gactacgcga
     2041 ccaacgacgg catcgccttc gggcagttgc aggagggcgg cgagttctca cgctggatgc
     2101 tcgtgacact ggccgcgctg gccgcggtcg ccgtgctcgt ctatttcttc cgcacgccgc
     2161 gcgcggagga tcgcgtgctc ggcgcgtgcg cgctgctgct ggccggcatc gccggcaacc
     2221 tcaccgaccg cgtgcgcctc ggccacgtca tcgacttcgt cgaagtgcat ctcggctctt
     2281 tccagtggcc gaccttcaac gtcgccgacg ccgccatctg cgtcggcgcg gggctgctcg
     2341 cgctcgacat catcctcgaa ggaaaagggg tcagggggaa aggggaaagg aaggaggcgg
     2401 agggcaccgc ctcgtgagag tgtgcctcct ttaccccttc cccttttccc tttaccctct
     2461 gacctgagat gtacccagag ctttttcgca tcggcggctt ccccgtcaac acctacggcg
     2521 tgcttctggc gctggccttc ctcgtcgcgc tttacgtcgc ctcgcgcctc ggcgagcgcg
     2581 acgggctgcg gcgcgagcgc atctacgatt tgggtttgtg gatgcttctg gcgggtctcg
     2641 tggggtcgaa gctgctgatg atgttggtcg agccggagta tgccgagaac cccgcgcggc
     2701 tgctgtcgct cgacttcctg cgttcgggcg gcgtgtggta tggcggcttc atcggcggcg
     2761 tcacggccgg ctactttctg atgcgccgct ggaaactgcc tttctggaag acggccgact
     2821 ccttcgtgcc cggcatcgcg ctcgcgcagg ccatcggccg gcagggctgc ttcgccgccg
     2881 gctgctgctg gggcgagccg acgctccgag cgtggggcgt acacttctcg gagcacggcc
     2941 actcggtgac gggcgtgccg acgactgtcg cgcaactggg cgcttccgag cgcgccgtct
     3001 gggccgagcg actgggcggg tttgacgcgc ccgtctatct acatccgacg cagctctacg
     3061 agtcgttcgc gatgctcgcg gccttcgtct tcctgctgtg gctccaccgt cgaaagcgtt
     3121 tcagcgggca ggtcttgctg gcctacgccg cgctctacgg cgccgtgcgc ttcacgattg
     3181 agttcttccg cgccgacccg cgcggcgaca tcgccggcct gacatcgctc accggcctct
     3241 ccacctcgca aatcatcagc ctcctcatcg gactcacggg agtaatcctc ctcgtcgtcc
     3301 gccggcggcg tgcgccttcc gcgcccgagt cggacggcga ggccgcggtc actaacacgg
     3361 cgagcgcgta aaagaaaact gtagccgggg tcggtcgagt caacggcccg ctcaagaggc
     3421 aagagattca gacaggatta acagggcgga caggatgaag aagagtagat ttgagatttg
     3481 agatttcaaa tgatctcatc ccgttagtcc tgcatatcct gtccatcctg ttaattcctt
     3541 cttggttggg gcagcggaca agtctgctgc ccagacaatc gacgcgggga cgtggtgcat
     3601 gagtgaggaa caacttatcg ggccggaggc ggagacgctc gcgtttcacg cctcggcgga
     3661 agaagcgggc gagcgcctcg acgcgttcct tgcggcgcgc atcgcgaccc agagccgcag
     3721 cgcgatcaag cggctcgtcg atgacggcga cgtgctcgtt gacggacgcg tcgtcaagcc
     3781 ttcctacaag ctgcgcggcg gcgaggagat cgaggtcgag acacccccgc ctccgccgct
     3841 cgaactcgcc ggcgaagaca tcccgctcga catcgtccac gaggacgacg acatcgtggt
     3901 cgtcaacaag ccggccggca tggtcgtcca cccggcggcc ggcgtcccct cgggcacgct
     3961 cgccaacgcg ctcgtttacc acttccaaca actcccgacg cgcggcggcg aggcccgccc
     4021 cggcatcgtc caccggctgg atcgcgacac gtcggggctg atcgtcgtcg ccaagacgcc
     4081 gcaggcgcac gaacacctct ccgagcaatt ccgcgtgcgc gaggtacaca agtcttacgt
     4141 cgcgctcgtc cacgggcgcg tcgagggcga gcgtgggcgc atcgaggagt cgctggcgcg
     4201 cgacccccgt caccgcacgc gcatggcggt gcgccgcggc gggcgcgcgg ccctcacgct
     4261 gtggcgcgtg cgccgacgct acggccgctt cacgctgctc gacgtggaga tcaagaccgg
     4321 acgcacgcac cagatacgcg tccacgcggc gtggatgaag caccccgtag tcggcgacga
     4381 gacttacaac gacgggcgcg accggagcat gtccgaccca gccttgcgcg cgcgcatccg
     4441 cgccatcggc cggcaattcc tccacgccga gcggctcgcc ttcgcccacc cgagcacggg
     4501 cgagcgcccg ctgtttacgg ccccgctccc gcctgagctt tccacctttc tcgacacgct
     4561 ggaggccgcc gaagcgacga cgcgcgaatg agcgtcaacg ccggtcatgg ctgtaggagc
     4621 ggggcacgtc cgcgaccctt cccggcgaaa gaattattaa catggatggg caggatgaac
     4681 aggatggaga aagagctgat ttaagatttg agatttcaga ttttaaatct tcatatcctg
     4741 cccatcctgc ccatccatgt taattttttc attggcaacg ggccgtggac gtgcccggcc
     4801 ccaagtatgc gcccgcgcgc tttcgttgtt atactgtgcc cctcccggcg cgaaccccaa
     4861 cgctgtaagc aacgactctc ctcgcggcgg gcatccaaaa cgcggatcac gagcgaaccc
     4921 aaaacgggcg cccctgaggc ggttccctcc ggctcgcagt ttcagttaat tatgagaaca
     4981 gccatctttt cgttcctcct cctgacgctc gccgcaggtc ttctgggcgg cggggccttg
     5041 tcgctcgcat atgagccgca gggagcgccg cagacgcccg cgccgcgcgc gacgcccacg
     5101 cccgttgacg acgacgatgt cgtgggcatc agaatcccgc tcgtgcgtct gccgatctcg
     5161 gtcgtggaca agaagggcca gcccgtggcg ggtctttcgg ccggcgactt tcagattttc
     5221 gaggacaaga aggcgcagca gttcgagttg ctctcccgtg ccgaggagtt ggagcggctg
     5281 ccggtctacg tcggcgtgct gatggacacc tcctcgtcca cggccggcaa gctcaagttc
     5341 gagaaggagg cggcgctcaa cttcgtccac acggtcgcgc gtctgcgtaa agaccgcgtc
     5401 gccttcctca ccttcgacca cgaggtcact ctgcggcagg actttacaga caagctcgac
     5461 ctgctcgaca aggccatcaa cgccgtcaag gcgccgggca ggcaaacctc tctctacgat
     5521 gccatctgga ttttctgcaa cgagaagatg cgcggcgtcg gctcggcgcg gcgcgcgctt
     5581 gtcgtcatca cggacggcga cgacacctac agccgcgcca cgctcaaaga cgccatcgac
     5641 atcgcgcaac gcaccgagac ggtcatcttc gccatctcga cgaagggcgg gttctcggga
     5701 agcgccgtcc ccggcgtcga ggcccagacg gtgaaggact cgggcgacaa agacctcgtt
     5761 aagctctgtg aggagacggg cggccgcgcc ttcttcacgg gcgacatgct cgctctggag
     5821 cgctccttca cgaccatcgc gaaggagata cgctcgcagt acatcgccac ctatcgcccg
     5881 tcgaacgacc gctacgacgg cagctaccga cgcatcgagg tcaagctcgc ctccgagcgc
     5941 gacggcctca aggtgcgcgc caagcgcggc tatctcgcgg tgtccgacac ggtgaagtga
     6001 gaaagaccgt gaatcgtcaa tcgtgaatcg tcaatcgacg ggttgctgcc cgaccacttg
     6061 cgattcattg ctcaggtcga gtcatcactc aaccacgatt cacggttgac gattgacgat
     6121 ttacggggtt ttacagatgt tccgtcgcag tcaaatcgct cgcgccctgg cgctcctgat
     6181 ttcgttcgcg tccgcggccg tcgccggcag cggcttgctg tcgtcgccgt cgagcgcgcg
     6241 ggcctcgcag tcgcacgccc agtcgcagga ggcggagacg agccggtcgc gccgcgccgg
     6301 attggcgcag gagggtgacg ctgccgccgc gccgtccgac cagcaacagc agacgacgca
     6361 gcccgccccg cgcccgacgc ccacacgccc cgacgaggag atcgacgacg acgaggtgct
     6421 gcgcgtcgag accgacctga cgaacgtgtt gttcacggcc gttgacaaga acaagcgctt
     6481 cgtcatcaac ctccggcagg aggacatcga ggtctacgag gacggcgcgc gccaggagat
     6541 tttcaccttc caacgcgaga ccgaccggcc gctctcgctc gccatcctca tcgacaccag
     6601 cgcctcgcag gagcgcacgc tccccgagga gaaatcggcg gcgcagcgct tcgtcgatgc
     6661 cgtcatccgc aaggccaagg atgaagtggc cgtgctctcc ttcaccggcg agacgacgct
     6721 ggagcaatct ctgacgggcg accccgcgcg cgtccgccgg gccatcgacc gggtggagtt
     6781 cgtcccgccc tcgggctaca tcggcggcgg cgtcatcgtc cccggcggca cgccccccat
     6841 ctccggcacc aaccagcgcc tcgccggctc gaccgccatc tgggactcca tctgggtcac
     6901 gtccaaagaa gttctctcag agtcgtccga caagacgcgc cgcgccatca tcctgctctc
     6961 tgacggcatc gacaccagca gccagctcaa gatggccgaa gccatcgacg gcgccatccg
     7021 cgccgacgcc gtcatctacg ccgtcggcat cggcgacaac ttcttcgacg gcgtcgatca
     7081 aggctcgatg cgtaagatcg ccgagcgcac gggcgggcgc gcctacttcc cccgcaacga
     7141 ggaagacctg cgcgtggcct tcgcgcagat tcaggaggag ctgcgctcgc agtatctcgt
     7201 cgcctactcg ccgacccgga aggcgaagga cggcaccttc cgcaacgtca agatcgaggt
     7261 cgtcaacccc gagctgcgcc ggcagaattt gcgcctgacc taccgtcagg gctacttcgc
     7321 acaaacggcc gccgccccct cccgcaagcc ttgagcgacg ctcggccgat tcgtattacc
     7381 gaccgtttag aggcgggcca tgcccgccgg ctcaagcaac gagtcttgag cc
//
LOCUS       DASVES010000153        43053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1168761, whole genome shotgun sequence.
ACCESSION   DASVES010000153 DASVES010000000
VERSION     DASVES010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 43053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43053
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168761"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1534
                     /locus_tag="VF240_05920"
     CDS             290..1534
                     /locus_tag="VF240_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7174802.1"
                     /translation="MSQGLRSSVQNTSARRTPARRFSYFIYTLLLVEFLDEFVYGARE
                     AAWPLVRDDLRLSYAQIGLALAAPGIVGIIIETFLGVLGDVWNRRALVLGGGLFFAAG
                     TLLVASSDSFTLLLVALCVLSPASGAFVGLSQAALMDAEPARREQNMARWAFSGSLGN
                     VAGPLALGAAVALGWGWRGLFLCLAALTLVVVVAAWRLPFPVPTAGVDDDDAVRHGLR
                     AGLRDALRALRRREVVRWLLLLEFGDFTWDVLRGFLALYFVDVVGVSLSEAAFAVFVW
                     TLVGLPGDFLIIPLLERVRGLSYLRVSTACVIVLFPAFMLAPGMTAKLFALGLLGLAN
                     AGWYSILKAQLYKEMEGRSGAVMTLQNVSGIISSTAPLALGAFAQRYGLEAMMWLLLV
                     GPLVLLAGLLTTRDDAGPAPSS"
     gene            1749..2945
                     /locus_tag="VF240_05925"
     CDS             1749..2945
                     /locus_tag="VF240_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7174803.1"
                     /translation="MSDELLEWRREFPILESCAYLISHSLGAMPRGAFGRLQDYAELW
                     ATRGVRAWAEGWWEMPLTVGDELGRIIGAEQGSVAMHQNVSVCQSLILSCFDTTPETR
                     RNKVVYSELEFPSVMYVYEAHARDGRYRIERVKSDDGVTASLERMLEAIDEETLLVPI
                     SHVLFKSAFLQDARAITERAHEVGALVVLDTYQSAGTVPFSVKDLDVDFATGGSVKWL
                     CGGPGAGYLYVAPRLRGALEPKVTGWMAHAAPFAFEDAPIRYADGAARFLHGSPAVPA
                     LYAAESGYKIINDIGVERIRAKSMRQTQRLIELCEEAGFQVTSPRDPERRGGTITVAV
                     PHAAAVTRELIRREIIVDYRPGAGVRLSPHFYTKDEELEGAVSEMRRIIDTRAYAEHE
                     AAGAAF"
     gene            complement(3154..4842)
                     /locus_tag="VF240_05930"
     CDS             complement(3154..4842)
                     /locus_tag="VF240_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX7174804.1"
                     /translation="MANNTKASVPSPSAPIWQNWSGNLVHNPSSDGTKYYFVPTNLAE
                     LKQVLAEVSKIKGATIRVSGQRHSQPPLVISDNRNAAPQMTKEYLVDMSCYADLGDAH
                     DQRLVLGPGENQVTLNAGVREDELDAFLTRHNLMMKTVTAGGFFSLGGMTAVDVHGGT
                     MDAPIFAETVAAFNLLLADGTVTTIDAQSPKVAGWSPLQFARVSLGGLGIVTSITLDA
                     MSRPYATTLQGGTERLGLADKTAFVKKFKTLIGQHTRLETFFTPYATSNVGFPLYTKN
                     FLALHWDVKDNPSPKTPNQPPSPEPPNACVLAGKQPPSYGAPYLGGFAKYGADAAQRA
                     QYVKSPGNTPLGVIEDGFDNPAVISAITFEVIEPDVATANKSHSEMWLTGAVRVIFMS
                     YYVPLPNLDDAGLGKVWDGLDVVSQIVLKDGNFHIAAPMEFRFVKGGDTAMSGTFTEA
                     PANTWFINLDLIGFVEYDQTARPRYTAKLLQFFADVERKWVAMGGFPHHGKMYGFYDP
                     TDESGKHTEPFNENFLADLRRRRGARQEAFDAYRKQLDPNGLFCNDFLCQLLEP"
     gene            4869..5072
                     /locus_tag="VF240_05935"
     CDS             4869..5072
                     /locus_tag="VF240_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174805.1"
                     /translation="MFVINHSVGESLWGVLNAALIVGLSKDAPLGLVPGDFVLGPWLG
                     LLGFVGVIVLLYGWMLRRSAAAR"
     gene            complement(5156..8029)
                     /locus_tag="VF240_05940"
     CDS             complement(5156..8029)
                     /locus_tag="VF240_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7174806.1"
                     /translation="MDEELWKQVDALLDAALDLPERERERFVAEATVGDPALRGEVLS
                     LLRAQSQASGFMERSAMKIAAQAMAQDSQLTSHQSLVGKDIGNYTIESLLGSGGMGEV
                     YLAREMKLNRRVALKILPRHFVADVERATRFKREALALSALNHPNLVTIYEVGEAGGL
                     NFIAMEFVEGQTLRPMMRNGLKLKDGLSIAGQVAEALAAAHASGIIHRDIKPDNIMVR
                     PDGYVKLLDFGLAKLTEAREADAAHTQAGAALGTLAYMSPEQAAGETTDHRTDIWSLG
                     VVLYEMVTGRKPFEGRERRVTVNAILSSEPDSASSIDPTLPSELDLILDKALEKDRDL
                     RYQTAADFRADLRRLLRAIESGTGSARGRAIEKSAGPTAPRARLVLPVAAVLVLAAVG
                     VAVWWFSQSPAATPLDWGRATHVQLTDQPGTEHFPTLAPDGKSFVYASRVKGNWDLFL
                     QRVGGKNATLLTPDTASDESQPAFSPGGDRIAFRSTREPAGIYVMEATGENARLVVAG
                     GHHPSWSPDGKEIAYSTAGRDVPSTRTTITSAIRVISLGTGATRLLTEADAMQPSWSP
                     NGHRIAYWFMPPNVGRSDIATVANGGGGEPVAVTKGASTNWNPVWSPDGRFLYFVSDR
                     NGNMSFWRVAVEEETGRVLGEPEAVVTPSAFNWHLSFSRDGQRLLYVQTSEQSNIRAV
                     EFNPESEKIVGQPFWVTRGDRRVVRPELSPNGKQFVMRVPRRSQDDIAVVNRDGRDWR
                     DVTNDKFFDRYPRWSPDGKRIAFASDRSGNYEIWSLDLEGTNLRQHTFSSESSASFPL
                     WSPDGKRLLFQSNFLNAIIDADKDWTHQTRDPLPAPEGETHFLAWDWSPDGGKLAGTF
                     DGAESAVGYYSFARRKYEKVAQVEGYPMWLSDPMWLSDSRRFVFSAGSKAYIADIVTK
                     RMLEVFDTGQDEIRGIGISNDDRLIYFSLYESESDIWLLDLRQ"
     gene            complement(8034..8603)
                     /locus_tag="VF240_05945"
     CDS             complement(8034..8603)
                     /locus_tag="VF240_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX7174807.1"
                     /translation="MDALESRDLTELLVAWSDGDEAALDRLAPLVHAELHRLASHYMR
                     RERGDHLMQTSALINEAYLRLIDWKAVRWQNRAHFFGAAAQIMRRILVDFARRRPRVA
                     QDVEARHISLEQAMTVTVEKDSDLLALDEALKELARIDERKSRIVELRFFGGLSVEET
                     AEVMSIAPVTVMREWNKAKAWLYRELSNA"
     gene            8883..9434
                     /locus_tag="VF240_05950"
     CDS             8883..9434
                     /locus_tag="VF240_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174808.1"
                     /translation="MKTKLTVMLLTLTGLMILAGQIGGGSLAATVDDQVNPHYKGGST
                     FNLVNAGPNVARCGAFPENIELSFTGSGIDTEGGYNTAVFSACTNTTTNLVFDLKATD
                     TYVQTGDQVFIEGDPFVLAPNPAICAAANAHGVPFRVAGGTGKYAGATGHGRFHIASN
                     LTPCNGQTPPSQVWFEGVIKWQQ"
     gene            complement(9672..10892)
                     /locus_tag="VF240_05955"
     CDS             complement(9672..10892)
                     /locus_tag="VF240_05955"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX7174809.1"
                     /translation="MRFSIKTIKLAFFFLFVPGSLYAFLVGGPTRAANSTRVHAYQPA
                     IPRGISPKLWRKLIPADNPMTAEKVALGESLYFDKRLSADGTVSCATCHDPATAFADN
                     KALAVGVSDKVGTRNAPTILNAMFNEQLFWDGRAPSLEEQAKQPLINPLEMGMPGYDV
                     LIARLSAIPEYRQGFRQVFGEEEITLDRIVKAIAAFERTQLTGNSPFDRFIEGDSPAL
                     TPAQKRGWELFRGKAKCSECHTFNSSAPFFTDAGFHNTGVATKNTNFDRLILAARKFQ
                     TSKPERRRAANTLAHTQAVSELGRYLVTGQAKDIGAFKTPTLRDIELTAPYMHNASEK
                     TLLDVVRFYNRGGEPNAHLDQRMRPLNLTDEEMNHLVEFMRALTSDEVMRRAQTARPQ
                     TRAAVFLNTPTARR"
     gene            complement(10951..11577)
                     /locus_tag="VF240_05960"
     CDS             complement(10951..11577)
                     /locus_tag="VF240_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174810.1"
                     /translation="MENLALIAEGLSVRTSGLADDDQPELRVRVRDAALLGECEAFLK
                     FVAAYIERGARLRPDETLAYGYWLTKFSLADDGLLEVWEHEAGATAFVIGAQLTLTYW
                     RDQHEVCRKFGGDFTPPRPDQLVVVSDGVFEGDTVQGVRYPSPEHMSGWWITTDRYDG
                     NTDSLKREHLYHLTAARPDLARLIALPFGFRFDLAHHEDVWFDRQTLG"
     gene            complement(11583..17369)
                     /locus_tag="VF240_05965"
     CDS             complement(11583..17369)
                     /locus_tag="VF240_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX7174811.1"
                     /translation="MKKSISLRVVLSVGLSLYMVVGPCLLPLQASGAGAQKAVAQTPP
                     AGLKRASANAANASIIRFLRVPAPQTETLQPGQSATLMPDGRWLLLGGEGADGPSRDA
                     QVKDARDGSVLPLGNGLQQARASHSATLLPDGTVLIFGGVGAGEEVIDSAELFHPDTG
                     QFELLPLKGLTARAHHTATLLTNGQVLIAGGTSAKGEMQARVELWEPLTKTVKPVPGQ
                     LQSLRRRHSATLQPDGAVLLWGGVGRDGANLDDGVLYEPKSEQLTWISGFNRFEDENA
                     PYLRASLPEDGTTDVPVETQIALRFSKPLSVTTVNAQTAALKGPQGTVEAKITPAEGG
                     MLAFIVPREKLLEETTYTITLADALDQNNRALTYTMLSFTTERGASSDARLADGEDWI
                     PDTRNMRGDWRSHRPESPWQKMPPLEAAPGVTALAGQVLLLNGHPLANVTLQIGARSA
                     RTDNTGRFLLTDLPAGHHVMRLDGATASKPQKVYGVFKIGVDVEAGKTNVLPFTSWMP
                     RLDTEHVTTLPVPTSREVAVTSPYIPGLEVRVPQGAVVRDIDGQPVSQLTITPIPVDR
                     TPFPLPGGVNVPVFFTVQPGASRVIPPRAQVVYPNYGNDRPGTRMNFWNYDPEDKGWY
                     IYGHGTVTSDGRQVMPDPGVVIYEFTGFMINRNGMPPPPGSGPNPGSDGTDGDPVDLA
                     TGLFVYDKTDLILPDTMPLKLNRTYRPLDPNSRPFGIGTSHPYELYLYSAQEYVEADL
                     ILPNGGRVHFVRISPGSGFTDAEFEHTTTSSPFYRSRLKWNGNGWDLKLKNGLTYVFG
                     DEAPLNTIKDRHGNKIIISRASTNGFGSPTGRVTKLTSSNGRWIEFTYDASNRITQAK
                     DNIGRTVNYTYDASGRLWKVTDPKAGVTEYTYDTSNRMLTIKDARAIVFLTNQYDANG
                     RVIKQTQADSTTYLFAYTLDANGRVTQTNVTNPRGEIRRTTFNADGHTLTDITALGKP
                     EQQSVTYERQAGTNLPLSTTDTLNRKTTYVYDTLGNATEVTRLAGTAQAATLRFTYDP
                     RFNQMASATDALNHTSSFEYDAQGNLTGIVNPLTQRTSMTYNTAGQPITLTDPLQNTV
                     QFEYEGGLLSSVTDQSGRTFRRFLDDAGRLLSVTAPLGQVTRYDFDALNQLVKSTDPL
                     GATTSYTYDANGNVLTVKDGRNNTTTFVYNNMDRLTSRKDPLLKTQTYQYDAAGKLTK
                     HTDRRGKVTTYTYDGIDRIVFIGYGTVTSQQATTYESTITNTYDAGSRLTKVVDTKAG
                     TINFTYDGLDRILSEVNPQGAITNVYDVAGRRTGMTVAGQTAVGYAYDDSNRVTGITQ
                     GASSVGLAYDAAGRRTTLTLPNGVSVEYGYNQVSQLTSLKYKKGAELLGDLAYEYDAA
                     GRRTKVGGSYSRTGLPQPLTSATYNAANQVTQRGATNLTYDANGNLTNDGANTYTWDA
                     RNKLVSISGATTASFVYDSFGRRVSKTVGAQATSYLYDGINTVQEKVGAAPSANMLVG
                     SLDEVFTRTTSAGTQTLIGDGMGTTLALLDATGAEQTRYTYDPFGAVTQTGGANDNPN
                     KFTGREDDGAGLYYYRARYYSPQQQRFISGDPIGFAGGDANLYAYVGNSPTNYADPSG
                     LILDTLLDIGFIAYDLYKLATGGRKNFWDNMGNLGLDVVGAAVPFLTGLGAGRRALQH
                     GDDVIDAARQAPNCFVAGTLVHTADGARPIEEIKAGDRVLSWNEESRQAEYQKVAQTF
                     VRRAEALILVTVEGAENAPLGTTDEHPFYVHRARDCLGDGDADEDDEGEWVGAAMLRV
                     GDHVRRPAGGWVRVLKVERRAESATVYNFEVENTHTYFVSALGVLVHNSCKTLQPGPH
                     AGESIPARGPQRDFTPQERSDINRIGQETGCHTCGSKDPGTKSGNHIPDHQPPNQLNP
                     NGGPQELYPHCLSCSRTQGGEVRQATRP"
     gene            complement(17565..18107)
                     /locus_tag="VF240_05970"
     CDS             complement(17565..18107)
                     /locus_tag="VF240_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX7174812.1"
                     /translation="MLRKSLINARPAALMLAALLLSVAASGGAGATAATQQGAQAPPP
                     QNIYFTAQNAGNFKTFIEAVKAAGLVDTLKGGGPFTVFAPTDEAFAKLSANTLEELLR
                     PENKEKLKDLLTAHIFPVKATASELNKLKDAKTLKRNSLQFDTSNGLKVGKAKITQAD
                     INTSNGVIHAIDTVLMPEGN"
     gene            complement(18219..26078)
                     /locus_tag="VF240_05975"
     CDS             complement(18219..26078)
                     /locus_tag="VF240_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX7174813.1"
                     /translation="MARTGSKLLSTFILLLVLALSAQAQTATYHLHKEASATTGLFQL
                     KTAGPDGTSLAVQSANLKGVAAGEYLVKAFDTPSGVPNSSGVISAGSNISFTLWMRKT
                     TTGGTIYPRAKLRLNGVAGTLLGTATSATALTSTLTKYTFTTTTSTNISVTAADRFYV
                     WVGVNLTAAPTTNTTAELDIEGTLNGNYNSLVTVPLPIRPTVDTISPSAGPTGTSVTV
                     SGSNFGATQGTSTITFNGVAAAPGSWSASTIVAPVPSGATTGPVVVTVNGAASNSSTF
                     SVGEISGISGSVARAIDGAPIVGAQVKAIQTGAVKGTATSGGDGSYSITNLLVGTYDV
                     EASAAGYETQSQSGQQVSAPTPATVSFALNSTQITYIYDELGRLVGVVDPNGERVTYA
                     YDAVGNLTSISRQSASQTSIIEFTPNSGAVGKQVIIYGSGFSSTPTQNTVKFNGVVAA
                     VASSTATQIVTNVPAGATTGTINVTTPSGTATSSATFIVGDPGLPTITSFTPSVGSPG
                     AAVTITGTNFQTVPSSNRVAFNGVRASVTSAAATSIAATVPTGASSGRITVTTPDGKA
                     TSSNDFYIPPSPYTGTDVESTGRIALGEVKTVSVNTASKVAMLLFDATAGQRISLDIQ
                     SSNIPGYTYVYIYKPDNTQLTNTYVYQNGAGFIDALTVPATGTYTLIVDPEGSNTGSL
                     TLRVNDATNVNGGTITPGGAAVTTTTTVPGQNAQVTFSGTAGQKISLDISAVTIPGTY
                     VYVQKPDGTTLAYVYSGTGGAFMDRQTLPASGTYTILVDPSNTYTGSATLRLYDVPAD
                     GNVPITPGGAPVTVTTTVPGQNATLTFTGVYGQRVSISISSSTIPSYAYIYLLRPDGS
                     QVTNTYVYQNGAGLIDTQVLPMSGTYTILVDPQAANVGSMTLQLNAVNDVTATITPGG
                     APATVTTTVAGQNARVTFDGTEGQRVSLSLTGVTIPGSYVSILNPDGSALVAAAHFGA
                     GGTFLDPRTLPVTGTYTILIDPTGGYTGNATLTLYEVPADGVGTITAGTPVTVTAAAP
                     GHNPRLTFTGTYGQRVSISISSSTIPSYAYIYLLRPDGSQVTNTYVYQNGAGLIDVQV
                     LSTPGTYTIMVDPQAANTGSMTLQLNDLSDVTATITPAGAPVTVTTTSAGQNAKVTFN
                     GTEGQRVSLSVTGVTIPSSYVSIISPDGSALVANTHFGTGGTFLDARTLPVTGTYTIL
                     VDPQSTHTGSATLTLYEVPADAVGTIASGAPVTVTAAAPGHNPRLTFTGTYGQRVSIS
                     ISSSTIPSYAYIYLLKPDGSQVTNTYVNQNGAGFIDVQTLPVSGTYTIMVDPQGANTG
                     SMTLQLNDASDIAAGTITPGGSAVTVSPNVAGQNARLTFSGTEGQRVSLNLTGVTIPG
                     SYVSILNPDGSTLVAATYSGTGGVFVEPRTLPTSGTYSIIVDPTGGNTGNATLTLYDV
                     PADVSGTLTPGAAPTTVTMGVPGQNSKLTFSGAYGQRVSISLSGVTIPSYSYVYLLKP
                     DGSQVTNTYINQNGVGLIDTQSLPLTGTYTVLVDPQGVNTGSMTLQLNDATDANAGTI
                     TPDGASVTASTTAAGQSARVTFSGTTGQRVSLSVTGVTIPGTYVYLLNPDGTSVANVY
                     TGTGGTFMDTQTLQSNGTYTILVDPQGTNTGSATLTLYNVPPDNAGTITPGGSTVTVT
                     AAAPGHNPRLTFTAVAGQRVSVNLSSVTIPSYSYVYLLRPDGSQITNNYVYQNGTASI
                     ATQTLQSAGTYTILVDPQGTNTGSMTVGLTDGGIVTVTPPVSDVTGTITPGGSAVTVT
                     TTTAGQNARLTFSGVYGQRVSVSISSSSITNYAYIYLLRPDGSQVTNTYVYLNGTGFI
                     DTQMLNATGTFTLLVDPEGSNTGSMTLQLNDTTEVNAGTITPGGAAVTTSVATPGKNS
                     VATFSGTAGQRVSLNVSGVTIPGSYVYLKAPDGATLAYVYTGTGGAFLDVQTLPGNGS
                     YTILVDPQGTYTGSITLTLHNVPADPSGTITPGGAAVTTTTTAAGQNARLTFNGVYGQ
                     RVSLSINSSTIPNYAYIYLLRPDGSQITNTYVYLNGTGFIDTQTLPVSGTYTILVNPE
                     GAGAGAMSLQLHDAAEVSTGTITPGGSAVTATTTAAGQKARLTFSGTAGQRVSLNISG
                     VSISGSYVYLLAPDGVSLANVYTGTGGAFLDTQALPANGTYTLLVDPQGTATGSATLQ
                     LYSVPADASGTITPGTPVTVTAAAPGHNPQLTFNGVYGQRLSISVSGATIPNYSYVYL
                     LKPDGSQVANTYVYQNGSALIDAQSLPISGTYMIRVDPNGAGTGNLTVQLNDVSDVGG
                     AITTGGAPVTVTTTSAGQNASLTFNGTEGQRVSLRISAVTIPGTYVYLKNPDGATLAY
                     VYSSTGGAFLDTQTLPTTGTYTIFVDPSSTNTGSATLTLFDVPADASGGITPGTPVTV
                     TAAAPGHNPRLTFSGVYGQRLSLSISGSTIPNYAYVYLLRPDGSQVTNTYVYLNGAGF
                     IDAQTLNANGTFTLLVDPEGANAGSMTLQLNDASDVNAGTITPGGAAATVTTTAPGQN
                     AKVTFDGVEGQAITVHLTSNTMSTVVVSLLTPDGMLITSATSYGGNLDLAPQTLPTTG
                     AFIIRIDPSGANTGGVSVNVTSP"
     gene            26068..26556
                     /locus_tag="VF240_05980"
     CDS             26068..26556
                     /locus_tag="VF240_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174814.1"
                     /translation="MRAMNWPSTVAESKLPLLVPRVSTVIALWSVKKPPRSMRWQTLA
                     CVEEIISYLLSSVKSRNYQQRNDRKLSANNSLTIGRAPPRSLDVCEVELSALSGELLK
                     LVSADCFESYVLQAVINKPVSVTLSSLCDANGRRVIEKRSHRPAQPKQIARNICAIDS
                     LR"
     gene            complement(26807..27799)
                     /locus_tag="VF240_05985"
     CDS             complement(26807..27799)
                     /locus_tag="VF240_05985"
                     /inference="COORDINATES: protein motif:HMM:NF044697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174815.1"
                     /translation="MSILIITRSDDNECVALVAEAVARRGGHAIRFDTDRYPTEVRLS
                     AYYGAAGDERLTLTNEAGEFDLREVTAVWHRRLNFGARLPATLDGQLRHASLGEASAA
                     AHGMLASLKAFRVDEVRHIRHAENKQLQLQAARDLGLDIPRTLTTNDPAAVRAFAKSC
                     EGGMVTKMLSSFAVYDEGRELVVFTNPVKPEDLADLSGLSLCPATFQELLPKSLELRV
                     TVVGRRVMSASLDSQSSARAAHDWRRDGLRMLQDWRPYQLPLEVEQKILRLMDYFSLN
                     YGAIDIILTPDARHVFLELNPSGEFFWLERTPGLPISEALADLLLGQAVLQKSS"
     gene            complement(27891..28916)
                     /locus_tag="VF240_05990"
     CDS             complement(27891..28916)
                     /locus_tag="VF240_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvdC family ATP-grasp ribosomal peptide
                     maturase"
                     /protein_id="HEX7174816.1"
                     /translation="MIPTTPRDAVLLLTHSGDFYTVDLVAQALARRGARPVRFNTDLF
                     PSSVKLSARAGDARAAHLYTDAGEQVSAAEVRAVWARKLWSPRMADDLDERYRLMCVR
                     ESVAALEGFLDALHDARWVNDLQRERDAENKQRQLRLAARAGLRVPRTLVTNDAAAAR
                     QFFAETEGRTVAKLLRPLTVGMDAAQPFVYTSRVSEEDLACAETLRHSPMVFQELIPK
                     ARELRVVCVAGRSLAGALDASGTSRAQTDWRRAAPGECRWQKAQLPAEVSSGLRALMS
                     ELGLVFGAVDLIHTPSGGHVFLEVNPSGEWGMLERDLGLPISEAIAESLLEVRGQRSE
                     VRRRTVS"
     gene            complement(29107..29301)
                     /locus_tag="VF240_05995"
     CDS             complement(29107..29301)
                     /locus_tag="VF240_05995"
                     /inference="COORDINATES: protein motif:HMM:NF033738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microviridin/marinostatin family tricyclic
                     proteinase inhibitor"
                     /protein_id="HEX7174817.1"
                     /translation="MRDQSEARAPEGSERSLPFFARFLESQDTPEVCAMTLKYPSDRD
                     EIDQRLEYASGNNETVAPHK"
     gene            29667..32186
                     /locus_tag="VF240_06000"
     CDS             29667..32186
                     /locus_tag="VF240_06000"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX7174818.1"
                     /translation="MKNLSRRLSPLAAFVFLLSAVASARAQSPELMDAAETQLALRRL
                     GVVGSALYVGAHPDDENTALLAYLANERGVRAAYLSVTRGDGGQNLIGTEQGAALGLV
                     RTQELLAARRIDGAEQFFTRAIDFGFSKSPEETLRVWGREAALADVVWVIRRFRPDVV
                     ITRFPTTGEGGHGHHTASAILAREAFDAAGDPARFPEQLDFVKPWKPKRLLWNAFVRQ
                     GERPANADKLLSVDVGKYNPLLGKSYTEIAARSRSMHKSQGMGAAERRGTALNYFAHV
                     AGEPASKDIFDGVDLTWRRVAGGEAVGQLLEQAEREFQASNPQGLLPHLFRARAEMRK
                     LPTGDALVNAKSNQLFKVILNSLGLWAEAVAAEPYATPGADVRVAVTLVNRSDVPARF
                     ESLALFSGQTHAVNKELKNNQPVTTEVLVPAPAEYSQPYWLRKEPGKGLFTVAEQESI
                     GGAEPTPHIPLVVRTTTGDGEKINFALPVLFRWTDRVQGEMYRPVVVVPEVAVRVEEK
                     VLVFPDRRPKQVRVRLKSNTAAAVSGTLRLRLPAGWTAAPAEVTVALKSKGEEFAAEF
                     AVTPPAAAATATLAAEFETNGKKFTRALVEIDYPHIPRQTLLPAAETRLVRVDLARRG
                     ARVGYVMGAGDEVPEALRQVGYDVVLLSDEDLEGADLSKFDAVVTGVRAYNTRAALRR
                     QQRRLLEYVERGGTLVVQYNTAERALEGFALGPYPVKLSQERVAVEDAPVTLVAPRDE
                     MLDAPNKITAADFDGWVQERGLNFPSEWDARYRTPFETHDPGETVKPGGTLIARHGRG
                     VYVYTSYSWFRQLPAGVPGAYRVFVNLISAGKQHSKAEAQP"
     gene            32281..32427
                     /locus_tag="VF240_06005"
     CDS             32281..32427
                     /locus_tag="VF240_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174819.1"
                     /translation="MPDTTNQHRDSGDERPPFGGSWAVLYAAVLLNLALLVVLFYLFT
                     RAFR"
     gene            32424..34106
                     /locus_tag="VF240_06010"
     CDS             32424..34106
                     /locus_tag="VF240_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX7174820.1"
                     /translation="MRPLDWLVLAAALVGIVLYGLWKGRRTRDLDDFLVADRRMRWHH
                     VALSIMATQASAITFLSTPGQAYTDGMRFVQFYLGLPVAMVILSVTAVPIYHRLKVYT
                     AYEYLEGRFDLKTRTLAALLFLALRGLSTGVSVYAPSLILSVIMGWDIRLVIFLIGGM
                     VLLYAARGGARAVDNTNFQQFLIIMGGMIVAFFTLVSLLPADVSFVEAVHVAGRLGRL
                     NAIDFSFDPSNQYNFWSGLLGGMFVALAYFGTDQSQVQRYLTGESVTQSRLGLLFNGL
                     VKVPMQFFILFLGAMVFAFYQFTEPPIFFNPVEVERARASVRGAQFSALEAEYSAAFA
                     AKETEARGLIGAMRVGDEAAEAAAAERLRAAQGRAGEVRAAAVEILKEQNPKPNDTNY
                     VFLSFVIHYMPAGVVGLVFAVVFFASMTSTASEWSALASTTVVDIQRRLVRRGASDRY
                     YLLSSRLATLFWGLFAVAFAQYAGHLGSLIEAVNRLGSLFYGTLLGIFLLAFYFKRTN
                     GTAAFTGALVGEGVVLYCFFFTKIAFLWYNVIGAFTVIATALVVNRAVGSKQ"
     gene            complement(34378..35331)
                     /locus_tag="VF240_06015"
     CDS             complement(34378..35331)
                     /locus_tag="VF240_06015"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX7174821.1"
                     /translation="MKPAQKFFRRAAALSLLLLTAAPLVSAQSPLRLPRPSPKASVMQ
                     TVGVTDVSITYSRPGVKSRKIWGDAPAGSAAGEATLDNQQTRPKDAVIVPYGHVWRAG
                     ANEATLFKVTDDVLINGQPLAAGSYSLHTIPGPAEWTIIFNKDDGQWGSFTYDAAKDA
                     LRVKTKPETAAVNQEWLAYSIEPEGETSARVNIRWEKLTVPFTVEVKDVKALTLVKAR
                     AAIAAAKPDDWQTPLQAANYALQNKLDLDEAMRWAEQSVKTKENFNNLNLRARILAAQ
                     GKTADAIAAGEKALAVGKAANANAQAMANFEKVVAEWKTKK"
     gene            complement(35465..37870)
                     /locus_tag="VF240_06020"
     CDS             complement(35465..37870)
                     /locus_tag="VF240_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7174822.1"
                     /translation="MSDGRVNPEQSSDELRALEELRRRERELSDFIENASVGLHWVGA
                     DGRILWANQAELDLLGYTREEYVGRHIAEFHADAAVINDILTRLVNKETLHNYEARLK
                     CKDGSIRDVLINSNVMWEEDKFIHTRCFTRDVTEAKQAEVELRESRSLLEDLLSREQS
                     ARAEAERQRDFTRLVLEHAPLAIGVMEGPAHRFLLVNRETCELTGLSEEQFTGRAHAE
                     VVPEADKVVAPILDRVYTTGLSETQEIDVVLPKGRRQLLVTWTAMPGRDGREGSVLYL
                     SLDISDRKQAEEALRESESQLRTLADAVPQLVWMADADGYIFWYNQRWYDYTGTTPEQ
                     MEGWGWQSVHDPEVLPKVLERWRASIETGELFEMEFPLRGADGVFRWFLTRVNPLRNS
                     QGRIVRWFGTNTDVDEQRRAAEELREANRLKDEFLATVSHELRTPLTAIMGWAHMLRT
                     GHFAGASADKALETIERNARTQAQLIDDLLDVSRIITGKLRLDVRSIDPNSFIEPAIE
                     ALMPAAEAKGVRVQKIMDTGVDSVSGDPVRLQQIVWNLLANAIKFTPKGGRVQVRLER
                     VNSHVEIAVSDNGAGIAPEFLPYVFDRFRQADQRTSRQHGGLGLGLAIVRHLVELHGG
                     AVRAESPGEGQGATFTVLLPVAPVYRNTGVDERVHPASRDTLPSYDCPERLDGLKVLV
                     VDDELDARELLRVGIGHCGAEVRTASSVQEALEAIENERPDLLISDIGMPGEDGYELI
                     RRVRELPAENGGRVPAIALTAYARTEDRLQALRAGYQMHVPKPVEMAELMAVAASLVG
                     RAG"
     gene            complement(38058..38426)
                     /locus_tag="VF240_06025"
     CDS             complement(38058..38426)
                     /locus_tag="VF240_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX7174823.1"
                     /translation="MRRTSTGLGLFALEPIPAGKRIIEYTGPLVSNEEVERRNWGKYF
                     FAVNTKWSIDGTPRSNTARYINHSCRPNAEAIVTGRRVWIWSKRRIKAGEEINYDYGK
                     EYFDDHIKPVGCKCEKCAAR"
     gene            38771..39616
                     /locus_tag="VF240_06030"
     CDS             38771..39616
                     /locus_tag="VF240_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX7174824.1"
                     /translation="MTEALSYNKYLRVQELIALQDCLSDPAHHDELLFITVHQAYELW
                     FKQILHETDAAVAAMDDDRTFAATRALARVVEIEKLLITQIHILETMTPISFLAFRDE
                     LNPASGFQSMQFREIEFSSGLRDKAILRAFRDDDFASPRLQQRHDAPTLAEAFYALLR
                     RRGLDAPADDLSAPEDERRKSYGRRTRAVLEILTHFEERPEEFQLSEALMAHDEYFSL
                     WRSHHVKMVERMVGSKPGTGGSEGVGYLRTTLDKKFFPEMWEARTHLDSKHGGAAGCP
                     FAGKS"
     gene            39665..41176
                     /locus_tag="VF240_06035"
                     /pseudo
     CDS             39665..41176
                     /locus_tag="VF240_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
     gene            41262..41675
                     /locus_tag="VF240_06040"
     CDS             41262..41675
                     /locus_tag="VF240_06040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7174825.1"
                     /translation="MPFSTSIAVRFADVDAAGLVYYPVLFHYCHVAMEEFFAARCGVA
                     YHELVAGEHIGFPTVSARAEFFAPLVYGDVAEVEVFVSNVGRSSATFEYRLRRASDRA
                     LCASATLVQVAMHLDARRAVPVPEPLRRAFAESVD"
     gene            41861..>43053
                     /locus_tag="VF240_06045"
     CDS             41861..>43053
                     /locus_tag="VF240_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7174826.1"
                     /translation="MHQRIYLSLLVALFLAVGPIARVGECVELSFAGAPAVVVSAEAA
                     DDTPSAKPKGNGFVRAITAPFRALGRLFSGGKKRDEAKKQRPVEETGARAVAAVTVVT
                     GPREEVKPEKRKEPAAARHTTPTPVAARGEVKKTAAAPDRQIAPGGGVAAEATAEVGP
                     SQGARVVRPAEGQPAPARLTPPKWIPVIEGIGRDPLTQGRALLQHGYPNEAVSELSIA
                     AVNGPNLGEANNLLGLAYDRLGLHAQAIDAYERSLSAAPDDAQVLNNLGYSLFLVGRH
                     AEALGRLKRAERLAPGSPYVLQNIAFVYARLGKYGDAFKTLARAFGDYDARLKTAELL
                     EQAGRNDDALKHYDAALKLRPVSPALLERIIAIYERTGRTREAEAARRTLGQPPNKQK
                     TATGGG"
BASE COUNT         7155 a        13338 c        14113 g         8447 t
ORIGIN      
        1 agcgggttgt agctacggga gcgtcaaccc gctcgctacc gcccgcggta ctgtgccggc
       61 atcacgctca cggattcaaa cgaaaggcac gcagatgaaa taccctccct ggtaagcctc
      121 agccgaagcc actgctgaac gcgtcgcaaa tcccgcggca cacgtgccgc gaaggctccg
      181 ctacgcgctc gcccgctcgg tgattccacc tccacggtca gacgttttca cctcacccgc
      241 aaccgaaaac gtcctcgaag aaaccttcgc cgtgaaagga gagtcaccct tgtcgcaagg
      301 actccgctcg tctgttcaaa acacttccgc ccggcgcacg ccggcgcggc ggttttctta
      361 ctttatctac acgctgctcc tcgtcgaatt cctcgacgag ttcgtctacg gcgcacgcga
      421 agcggcctgg ccgctcgtgc gcgacgacct gcgtctgtct tacgcgcaga tcggcctggc
      481 gctcgccgcg ccggggatcg tcggcatcat catcgagacc ttcctcggcg tcctcggcga
      541 cgtgtggaac cgccgcgcac tcgtcctcgg cggcggactc ttcttcgcgg ctggcacgtt
      601 gctcgtcgcg tctagcgaca gcttcacgtt gctgctcgtc gcgctgtgcg tgttgtcgcc
      661 ggcgtcgggc gcgttcgtcg gtctctcgca ggcggcgctg atggacgcgg agccggcgcg
      721 gcgcgagcag aacatggcgc gctgggcctt ctccggctcg ctgggcaacg tggccgggcc
      781 gctcgcgctc ggggccgccg tcgcgctcgg ctggggttgg cgcggcctgt tcctttgcct
      841 ggcggcgctc acgcttgtcg tggtcgtggc cgcgtggcgt ctgcccttcc ccgtccccac
      901 agcgggcgtt gacgatgacg atgcagtgcg gcacggactg cgcgccggac tccgcgacgc
      961 tttgcgcgcg ctccggcggc gcgaggtcgt gcgctggctt ttgcttttgg agttcggcga
     1021 cttcacgtgg gacgtgctgc gcggcttcct cgcgctctac ttcgtggacg tggtcggcgt
     1081 gtcgctctcc gaggccgcct tcgccgtctt cgtgtggacg ctcgtgggtc tgcccggcga
     1141 cttcctgatc atcccgttgc tggaacgcgt ccgcggcctg agctatctgc gcgtgagcac
     1201 ggcctgcgtc atcgtcctct tccccgcttt catgctggcg ccggggatga cggcgaaact
     1261 cttcgcgctc ggactgctcg gcctcgccaa cgccggctgg tactccatcc tcaaggcgca
     1321 gctctacaaa gagatggagg ggcgcagcgg cgccgtgatg acgctccaga acgtctccgg
     1381 catcatcagc tcgacggcgc cgctcgcgct cggcgccttc gcccagaggt acggcctcga
     1441 agcgatgatg tggctgctcc tcgtcggccc cctcgtgctg ctcgcggggc tgctcacgac
     1501 gagagacgac gcggggccag cgccgtcttc ataaacaatt aacggggcca cgcgcacgcg
     1561 tcggccttcc cttttccgat ggtagccaag cgcgtcgccc gccgatatac ttgaggccct
     1621 tagaaacagt accgcgagcg gtagcgagcg ggtacgcccg tgatgagagg cccgcctcgc
     1681 gcatcgacac cctcgaggct cccgctcgcg gtactgtccc ccgtaatcaa cacgcgagga
     1741 ggcgcttctt gtcggacgag cttctggaat ggcggcgcga gttcccaatt ttggagagct
     1801 gcgcttatct catctcgcac agtctgggcg cgatgccgcg cggcgctttc gggcgtctgc
     1861 aagactacgc cgagttgtgg gcgacgcgcg gcgtgcgggc gtgggccgag gggtggtggg
     1921 agatgccgct cacggtgggc gacgagttgg ggcgcatcat cggggccgaa caaggctccg
     1981 tcgccatgca ccagaacgtc tcggtctgcc agtcgctcat cctctcctgc ttcgacacga
     2041 cgcccgagac ccggcgcaac aaggtcgtct actctgagct tgaattccct tcggtgatgt
     2101 acgtctatga ggcgcacgcg cgcgacgggc gctaccgcat cgagcgtgtc aagtccgacg
     2161 acggcgtcac ggcgtcgctc gaacggatgc ttgaagcgat tgacgaggag acgctgctcg
     2221 tccccatctc gcacgtcctc ttcaagagcg cgttccttca ggacgcgcgc gctatcacgg
     2281 agcgcgcgca cgaggtcggc gccctcgtcg tcctcgacac ctaccagtcg gccggcaccg
     2341 tccccttcag cgtcaaagac ctggacgttg atttcgccac gggcggctcg gtgaagtggc
     2401 tctgcggcgg ccccggcgcg ggctacctct acgtcgcgcc gcgcctgcgc ggggcgctgg
     2461 agccgaaggt cacgggctgg atggcgcacg ccgcgccctt cgccttcgag gacgcgccca
     2521 tccgctacgc cgacggcgcc gcgcgcttcc tccacggcag cccggccgtc cccgcgctct
     2581 acgcggccga gtccggctac aaaatcatca acgacatcgg ggtcgagcgc attcgcgcca
     2641 agagcatgcg gcagacgcag cgactcatcg aactctgcga ggaggcgggt tttcaagtca
     2701 cctccccgcg cgaccccgaa cggcgcggcg gcaccatcac cgtcgccgtc ccgcacgcgg
     2761 cggccgtcac gcgcgaactc atccgccgtg agattatcgt cgattaccgc cccggcgccg
     2821 gcgtccgcct ctcgccccac ttctacacga aggatgagga acttgaaggg gccgtcagcg
     2881 agatgcgccg aatcatcgat acccgcgcct acgccgagca cgaggccgcg ggcgcggcat
     2941 tctaaaagca gtgacgagtg acgagtaaga cgagctttga caactcgtca ctcgtcactc
     3001 gtcactttct gtatggccga agatacggcg acggcggcct gctctcgcga atccgccgac
     3061 aggctgctca gttgtctacg agcgcgggcg agttatcaac cgggaccttc cggcgcgggt
     3121 cggggatgtg aatctactgc cctccagttc tccctacggt tcaagcagtt gacacagaaa
     3181 gtcattgcag aagaggccgt tcggatcgag ctgcttgcgg taagcgtcga aggcttcctg
     3241 gcgtgcgccg cggcgtcgcc ggaggtcggc cagaaagttc tcgttgaaag gttcggtatg
     3301 cttgcctgat tcgtcggtcg ggtcgtagaa gccgtacatc ttgccgtgat gcgggaagcc
     3361 acccattgcg acccatttcc tctcgacatc ggcgaaaaac tgtagaagct tggcggtata
     3421 ccgggggcgc gccgtctggt catactccac gaagccgatc aggtcgagat tgatgaacca
     3481 ggtgttggcg ggggcttcgg tgaacgtccc cgacatggcc gtgtcgccgc ccttgacgaa
     3541 gcgaaactcc atcggcgccg cgatgtggaa gttgccgtct ttcaagacga tctgggaaac
     3601 gacatccagt ccgtcccaaa ctttgccgag gcccgcatcg tcgaggttgg gcagcggtac
     3661 gtagtaagac atgaaaatga ctcgcaccgc gcccgtcagc cacatctccg aatgcgactt
     3721 gttggccgtg gcaacgtccg gctcgatcac ctcgaaggtg atggccgaga taacagcggg
     3781 attgtcgaag ccatcttcaa taacaccaag tggggtattg ccagggcttt ttacatactg
     3841 tgcccgctgg gccgcgtcgg cgccgtattt agcaaatccc cccagatatg gtgcgccgta
     3901 tgagggtggt tgtttgccgg caaggacaca cgcattgggt ggctcagggc ttggcggctg
     3961 gttcggagtt ttcggagacg ggttgtcttt cacgtcccaa tgcagcgcga ggaagttctt
     4021 ggtgtacaaa gggaacccga cattacttgt ggcgtaaggg gtgaagaacg tttccagccg
     4081 cgtgtgctgt ccgatgagcg tcttgaattt cttcacgaag gccgttttgt cggcgagacc
     4141 taagcgctcc gtgccgccct gaagcgtcgt cgcatacggg cggctcatgg catcgagggt
     4201 gatcgacgtg actatcccca gaccgcccaa agacacgcgg gcgaactgca aaggcgacca
     4261 gccggcgacc ttcggcgact gcgcatcaat cgttgtcacc gtcccatcgg caagcaggag
     4321 gttgaacgcc gccacggttt ccgcgaagat cggcgcgtcc attgtgccgc cgtgcacgtc
     4381 aaccgcggtc atcccgccga ggctgaaaaa gccgccggcc gtaaccgttt tcatcatgag
     4441 gttgtgacgg gtcaggaagg cgtccagctc atcctcgcga acgcccgcgt tcaacgtgac
     4501 ctgattctct cccggcccca gcacgaggcg ctggtcatgt gcgtctccca aatccgcata
     4561 acacgacata tccacaagat actccttagt catctgcggc gccgcgttac ggttgtcact
     4621 gatcaccagc ggcggctgcg aatgccgctg gccggagact cggatggtcg cgccttttat
     4681 ctttgagacc tcggcgagca cctgcttcaa ttcggcgagg ttggtcggca cgaagtagta
     4741 tttcgtgcca tcagacgagg gattgtggac cagatttccg ctccagttct gccagattgg
     4801 agccgaaggg gaggggacgc ttgcttttgt attgttcgcc attcttacct tccttcctgg
     4861 ggtcttcggt gttcgtaata aaccattccg tcggcgagag cctgtggggc gtgctcaacg
     4921 ccgccctcat cgtcggcctc tcgaaagatg cgccactcgg cctcgtgccc ggggactttg
     4981 tgctcggccc ctggctcgga ctgctcggat tcgtcggcgt gatcgtcttg ctgtacgggt
     5041 ggatgctgcg tcggtcggca gcggcccggt agcttgggga gacagcttcg attaccagag
     5101 ggcgtctgaa gagcaggctt ttccgcacgc cctctggtcg ttcactctgt ggcccttact
     5161 gccgcaagtc caagagccag atgtcgcttt ccgattcgta aagcgaaaag taaatcagcc
     5221 ggtcgtcgtt cgaaatcccg attccgcgaa tctcgtcctg cccagtgtcg aagacttcca
     5281 gcatgcgttt tgtcacgatg tccgcgatgt aggccttact tccggcggag aagacaaagc
     5341 gccggctgtc tgacagccac atcgggtccg acagccacat cggatagcct tcgacttgcg
     5401 ccactttttc gtacttgcgg cgcgcgaatg aataatagcc gaccgccgac tcagcgccgt
     5461 cgaatgttcc cgccagcttt cccccgtccg gcgaccagtc ccacgccagg aagtgtgtct
     5521 cgccctcggg cgccgggaga gggtcgcgag tctgatgcgt ccagtcctta tcggcgtcga
     5581 tgatcgcgtt caggaagttg ctctgaaaga gtaggcgttt gccgtcgggc gaccagagcg
     5641 gaaacgacgc gctgctctca ctgctgaagg tgtgttggcg caggttcgtg ccttccaggt
     5701 cgagcgacca gatttcgtag ttcccgctgc ggtcggacgc gaacgctatc cgcttgccgt
     5761 cgggcgacca acgcgggtag cggtcgaaga acttgtcgtt cgtcacgtcg cgccagtcgc
     5821 ggccgtcgcg gttgacgacg gcgatgtcgt cctgagaccg gcgggggacg cgcatgacaa
     5881 actgtttgcc gttgggcgaa agctcggggc gcacgacccg ccggtcgccg cgagtcaccc
     5941 agaagggttg accgacaatt ttctcgctct ccgggttaaa ctcgacggcc cggatgttcg
     6001 attgctcgct tgtctgaacg tagagcagac gctggccgtc gcgcgagaaa ctcaaatgcc
     6061 agttgaaagc cgaaggcgtc accacggctt ccggctcgcc caggaccctg ccggtctctt
     6121 cctcgacggc gacccgccag aaactcatat tgccgttccg gtcgctcacg aagtaaagaa
     6181 acctgccgtc gggcgaccag accggattcc agttcgtgga ggcgcctttc gtcaccgcca
     6241 ccggctcgcc gccgccgccg ttcgcaacgg tcgcgatgtc gctacgcccg acgttcggcg
     6301 gcatgaacca gtacgcgatt cggtggccgt tcggcgacca ggacggctgc atggcgtcgg
     6361 cctcggtgag cagtcgtgtg gcgcccgtcc ccaaactgat aacccggatc gcgctggtaa
     6421 tggtcgtccg cgtgctgggg acatcgcgcc cggcggtgct gtaagcgatc tctttcccgt
     6481 ccggggacca tgaagggtga tgaccgccgg cgacgacgag gcgggcgttc tcgcccgtgg
     6541 cctccatgac gtagatgccc gccggctctc tggtcgagcg aaacgcgatg cggtcgccgc
     6601 ccggagaaaa cgccggctga ctctcgtcgg aagccgtgtc cggcgtgagc aatgttgcgt
     6661 tcttgccgcc gacgcgttgc agaaagaggt cccagttccc cttgacccgg ctcgcgtaaa
     6721 cgaaactctt tccgtcaggc gcgagagtcg ggaagtgttc tgtccccggc tggtctgtga
     6781 gttgaacatg agtggcgcgc ccccagtcga gcggcgtcgc cgccggcgat tgcgagaacc
     6841 accaaacggc gacacccacc gccgcgagca cgagaaccgc cgcgaccggg agaaccagcc
     6901 gggcgcgggg cgccgtcggg ccggcagatt tttcaattgc ccttccgcgc gcgctccctg
     6961 tgccggattc gattgcgcgc agcagccggc ggaggtcggc gcgaaagtcg gccgcggtct
     7021 ggtaacgaag atcgcggtct ttctcaagcg ccttatcaag tatcaaatcc aattccgagg
     7081 gaagcgtggg atcgatggaa ctggcagaat ccggctcgct cgacaggatg gcgttaactg
     7141 tcacacgcct ctccctcccc tcgaaaggtt tgcgtcccgt gaccatctcg tagaggacga
     7201 cgccgaggct ccagatgtcg gtgcggtggt cagtcgtttc gcccgccgcc tgctcgggcg
     7261 acatgtaggc gagcgttccc agcgccgcgc ccgcctgcgt atgtgctgca tcagcctccc
     7321 gggcttcggt cagcttcgcc agaccgaagt cgaggagttt cacatagccg tcgggccgca
     7381 ccatgatgtt atccggtttg atgtcgcggt gaatgatgcc cgaagcgtgg gcggcggcga
     7441 gggcctccgc gacctgcccc gcgattgaca aaccgtcttt gagcttcagc ccgtttctca
     7501 tcatcgggcg gagcgtctga ccctcgacga actccatcgc gatgaaattg agtccgccgg
     7561 cttcgcccac ttcgtaaatg gtgacgagat tcgggtggtt caacgccgac agcgccagag
     7621 cttcgcgctt gaagcgtgtg gcccgttcca cgtcggcgac gaaatggcgc gggagaattt
     7681 tgagcgccac cctgcggttg agcttcatct ctcgcgcgag gtaaacctca cccatcccgc
     7741 ccgaacctaa caggctctcg atggtgtaat tcccgatgtc tttgccgacg agtgattggt
     7801 gggaggttag ttgagagtcc tgcgccatgg cttgcgccgc gatcttcatc gcggagcgtt
     7861 ccatgaaccc agacgcctgt gattgcgccc ggagcaggct caacacttca ccgcgcaggg
     7921 cggggtcccc gacggtcgcc tccgctacaa atcgttcgcg ctcacgctcc ggcagatcga
     7981 gagccgcgtc aagcagggcg tctacttgtt tccacagttc ttcgtccatc ggctcaagcg
     8041 ttactcagtt ctcgatacaa ccatgcctta gctttgttcc attcgcgcat cacggtgacg
     8101 ggcgcgatgc tcatcacctc ggccgtctct tcgaccgaca gcccgccgaa gaaacgcagt
     8161 tccacgatcc ggctcttacg ctcatcaatc cgcgccaact ccttcagcgc ctcatcgagc
     8221 gcgagcagat cagaatcttt ttccacggtt acggtcatcg cctgctcaag cgagatgtga
     8281 cgcgcctcca cgtcctgggc gacgcgcgga cgccggcgcg cgaagtccac aaggatgcgg
     8341 cgcatgatct gggccgcggc gccgaagaag tgcgcgcggt tctgccagcg gacggccttc
     8401 cagtcaatca aacgcaaata ggcttcgttt atcagcgcgg aggtttgcat gaggtggtcg
     8461 ccgcgctccc gcctcatgta gtggctcgcg aggcggtgca attccgcgtg aacgagcggg
     8521 gccagccggt cgagggcagc ctcatcgccg tcactccagg cgactagaag ttcagtcagg
     8581 tcgcgggact cgagcgcgtc catacgtggc tacttgtaaa aaaagaggct atctaaaatt
     8641 agcgaggcgg gtgcggggaa cgtatccaat ttaccatacc gagccatgtg cttcgcaccc
     8701 tgagaagggt tcacgatctc acacgtgaaa attcatttcc ctcgatgata gttttcgcgc
     8761 gagatttacg ccttaggggt tggaagcgca ctgtcggctt cgaatggttt tctcaagcgc
     8821 gtgggcaacg actgagaata aacaaaatca caagttacaa ccccaaaagg agacctaaac
     8881 gaatgaaaac caaactcaca gtaatgcttt tgacgctcac cgggctaatg atactcgccg
     8941 gccagattgg cggaggaagt ttggcagcca cggtggacga tcaagtcaat ccccactaca
     9001 aaggcggctc aaccttcaat ctggtcaatg ccggaccgaa tgtcgctcgg tgcggggcat
     9061 ttccagaaaa catcgagctg agtttcacag gctcgggaat cgacacggaa ggcggctaca
     9121 acaccgccgt tttctctgcg tgtaccaaca ccacaaccaa tctcgtcttc gacctgaaag
     9181 cgaccgatac gtatgtgcag accggcgatc aggtcttcat cgaaggagac cccttcgtgc
     9241 tggcgccgaa ccccgccatc tgcgccgccg cgaatgctca cggcgtacct ttccgtgtcg
     9301 ccggcggcac cggaaagtac gcgggggcga cgggccacgg acgtttccac atcgccagca
     9361 acctgacccc gtgtaacggg caaacgccgc cgtcgcaagt ttggtttgag ggagtgatta
     9421 agtggcagca gtaagcacgc ccgttgtgtc ctcaagtttc cacggcgcgc gcaaggtcga
     9481 gggttggagg tggcgctcgg ccgccacctc cgcctcacgt tcgcgcccgc gcccgcgccg
     9541 cctcggccgc cgccaactcg tccccggcct ccatgcagtc actcaatgac aatatgcacg
     9601 ccggcgctac ttcgggcgaa actcatgtct tccgtgaccg gggcaggcga ggttcgtcgc
     9661 ttctctgacg ttcagcgccg ggcggtgggc gtgttgagga agacggcggc tcgcgtctga
     9721 gggcgggcag tttgagcgcg tcgcatcact tcatcactcg tcagcgctct cataaactcg
     9781 acgaggtggt tcatctcttc atccgtcaaa ttaagcgggc gcattctttg gtcaagatgg
     9841 gcattcggct cgccgccccg attataaaac ctgaccacgt cgaggagggt cttctccgag
     9901 gcgttgtgca tgtagggcgc cgtcaattct atatctcgca aggtgggggt tttgaaagcg
     9961 ccgatgtctt tcgcctgccc ggtgacgagg tagcggccga gttcggagac agcctgtgtg
    10021 tgggccagcg tgttggccgc gcggcggcgt tcaggtttcg acgtttgaaa ctttcgggca
    10081 gcgaggatca gacggtcgaa gtttgtgttc tttgtcgcca cgccggtgtt gtggaagccg
    10141 gcgtcggtga agaacggcgc ggaagaattg aacgtgtgac actcgctgca tttcgccttc
    10201 cctctgaaca actcccatcc gcgcttttgc gccggcgtga gggcaggcga gtcgccttcg
    10261 ataaatctgt cgaaaggtga gttgcccgtg agttgcgtgc gctcgaaggc ggcgatggct
    10321 ttcacgattc tgtcgagcgt aatctcttcc tcaccgaaga cttgtcggaa gccctgccgg
    10381 tattcgggaa tcgcgctcaa acgcgcgatc agcacgtcat aaccgggcat ccccatctcc
    10441 agcggattga tgagcggctg cttcgcctgc tcttcgagtg aaggcgcgcg gccatcccag
    10501 aacaactgct cattaaacat cgcgttgagg atggtcgggg cattacgcgt gccaactttg
    10561 tcgctgacgc cgacggcgag cgctttgttg tcggcgaagg ccgtggccgg gtcatggcag
    10621 gtcgcacaac tgacggttcc gtccgccgat agacgcttgt cgaaatagag actctcgccg
    10681 agcgcgacct tctccgccgt catcggattg tcggcgggga tgagcttccg ccagagttta
    10741 ggagagatgc cgcgcgggat ggcgggctga taagcatgga cgcgggtcga gtttgcggcg
    10801 cgggtcgggc cgccgacgag gaatgcgtag agactccccg gaacgaagag gaagaagaag
    10861 gccagcttga ttgttttaat cgaaaaccgc atggacttaa ttcttcggtg cggcagtaac
    10921 ttcaaggtca ctgcctcaga cagtcctcta ctatcccagc gtttgccggt cgaaccagac
    10981 gtcttcgtga tgcgcgaggt cgaagcgaaa gccgaacggc agcgcaatca gacgggcgag
    11041 atcgggccgt gcggcggtga ggtgatacag gtgctcccgc ttgagggaat cggtattgcc
    11101 gtcgtacctg tcggttgtta tccaccaccc ggacatgtgc tccggcgacg gatagcggac
    11161 gccctgcact gtgtcgccct cgaacacgcc atccgagacg acgacgagtt gatcgggtct
    11221 gggcggagta aaatcgcccc cgaacttgcg gcacacctca tgttggtcgc gccagtaggt
    11281 gagggtgagc tgagcgccga tcacaaaagc ggtcgcgccg gcttcgtgct cccacacctc
    11341 aagtaaaccg tcgtcggcca ggctgaattt ggttaaccaa tacccgtagg cgagagtctc
    11401 atcggggcga aggcgcgcgc cccgttcgat gtaggccgcc acgaatttga ggaacgcctc
    11461 gcactccccg agcagcgccg cgtcccggac gcgcacacgc agttccggct ggtcgtcgtc
    11521 ggcgaggccg ctcgttcgca cgctcagtcc ctccgcgatg agagcaaggt tctccattcg
    11581 tctcaaggtc gtgtcgcttg ccgtacctca cctccctgcg tccgactaca tgagaggcaa
    11641 tgcgggtaca actcctgcgg gccgccgttc ggattaagtt gattgggcgg ctggtgatca
    11701 gggatgtggt tgccgctctt ggtgccgggg tctttgctcc cgcaggtgtg gcaacccgtc
    11761 tcctgaccaa tccggtttat atcagagcgc tcctgcggcg tgaagtcgcg ctgcggcccg
    11821 cgcgccggga tggattctcc ggcgtgtggg ccgggctgaa gagtcttgca ggaattatgg
    11881 acgaggactc cgagggcgga gacgaagtaa gtgtgtgtgt tctcgacctc gaagttgtag
    11941 acggtcgcgc tctccgcgcg ccgctccacc ttgagcacgc gcacccaacc gcccgcgggg
    12001 cggcgcacgt ggtcgccgac gcgcaacatg gccgcaccga cccactcgcc ctcatcgtct
    12061 tcgtcagcat caccgtcgcc gagacagtcg cgggcgcgat gaacgtagaa cgggtgctcg
    12121 tccgtcgtgc cgagcggcgc gttctccgcg ccctcgaccg tgactaagat caacgcctcc
    12181 gcacgccgca cgaacgtctg cgccactttt tgatactctg cctgccggct ctcttcattc
    12241 caggagagca cgcggtcgcc cgctttgatt tcctcaatcg ggcgggcgcc gtccgcggta
    12301 tgcacgagcg tccctgccac gaagcaattg ggggcttggc gggcggcgtc aatcacgtcg
    12361 tcgccgtgct ggagcgcgcg acggccagcc ccgaggccgg tgaggaaagg cactgccgcc
    12421 ccgaccacgt cgaggccgag attgcccatg ttgtcccaga aattcttgcg cccgcccgtc
    12481 gccagtttgt agaggtcgta ggcgataaaa ccgatgtcga ggagcgtgtc gaggatgagg
    12541 ccgctggggt cggcgtagtt ggtcggcgag ttgccgacgt aggcgtagag gttggcgtcg
    12601 ccgccggcga agcctatcgg gtcgccggag atgaagcgtt gctgttgcgg cgagtagtag
    12661 cgcgcgcggt agtaatagag tcccgcgccg tcgtcttcgc ggccggtgaa tttgttcgga
    12721 ttgtcgttgg cgccgccggt ctgcgtgacc gcgccgaagg ggtcataggt gtagcgggtt
    12781 tgctccgcgc ccgtcgcgtc gagcagggcc agcgtcgtgc ccatgccgtc gccgatgagc
    12841 gtctgcgtgc ccgcgctcgt cgtgcgggtg aacacttcgt cgagactgcc gaccagcatg
    12901 ttggccgacg gcgcggcgcc gaccttctcc tgcacggtgt taatcccgtc gtagaggtat
    12961 gaggtcgcct gagcgccgac ggtcttgctg acgcgcctgc cgaaagagtc gtagacgaag
    13021 ctggccgtcg tcgcgccact gatgctgacg agtttgttgc gcgcatccca cgtgtaggta
    13081 ttcgcgccgt cgttcgtcag gttgccgttg gcgtcataag tcaggttggt ggcgccgcgt
    13141 tgggtcacct gattggccgc gttgtaagtg gccgaggtga gcggctgggg cagccccgtg
    13201 cgcgaataac tgccgccgac cttggtgcgc cggcccgccg cgtcgtattc gtaggccagg
    13261 tctccgagga gttccgcgcc cttcttgtac ttgaggctgg ttaactgtga gacctgattg
    13321 tagccgtatt cgaccgagac gccgttcggg agcgtgaggg tggtgcggcg ccccgcggcg
    13381 tcgtaagcga ggccgacgct gctcgcgccc tgcgtgatgc cggtcactcg attggaatcg
    13441 tcgtaggcgt agcccacggc cgtctgcccc gcgacggtca tgccggtgcg ccggcccgcc
    13501 acgtcgtaga cgttcgtgat ggcgccttgc ggattcacct cggagagaat gcggtcgagg
    13561 ccgtcgtagg taaagttgat cgtgccggcc ttcgtatcaa ccaccttcgt caggcgactg
    13621 cccgcgtcgt aggtgttggt gatggtgctc tcgtaggtgg tcgcttgctg gctggtgacc
    13681 gtgccatagc cgatgaagac aatccggtcg atcccgtcgt aggtgtaggt cgtcaccttc
    13741 ccgcggcgat ccgtgtgctt cgtcagcttc ccggcggcgt cgtactggta ggtctgtgtt
    13801 ttcaacaacg ggtctttgcg gctggtcagc cgatccatgt tgttgtagac gaaggtcgtc
    13861 gtgttgttgc gcccgtcctt cacggtcagg acgttgccgt tcgcgtcgta agtgtaggag
    13921 gtcgtcgcgc cgagcgggtc ggtggatttg actagctggt tcaacgcgtc gaagtcgtag
    13981 cgtgtgacct gcccgagcgg ggcggtgacg ctgaggaggc gtccggcgtc gtctaagaag
    14041 cgccggaagg tgcgccccga ctgatcggtg acgctggaga gcaggccgcc ctcatactcg
    14101 aactgcaccg tgttctgcaa cgggtcggtg agcgtgatgg gttggcccgc cgtgttgtaa
    14161 gtcatcgacg tgcgctgggt gagcgggttg acgatgccgg tcagattgcc ctgcgcgtca
    14221 tactcgaagc tcgacgtgtg gttgagcgcg tccgtcgcgc tcgccatctg gttgaacctc
    14281 ggatcgtagg tgaagcgcag ggtggcggcc tgggccgtgc cggcgaggcg cgtgacctcg
    14341 gtggcgttgc cgagggtgtc gtagacgtag gtggtcttgc ggttgagcgt gtcggtcgtg
    14401 ctcaacggca gattggtgcc ggcttgccgt tcgtatgtga cgctctgttg ctcgggcttg
    14461 ccgagggccg tgatgtcggt cagcgtgtga ccgtcggcgt tgaaggtcgt gcgacggatt
    14521 tcgccgcgcg ggttggtgac attggtctgc gtgacccgac cgttcgcgtc gagcgtgtag
    14581 gcgaacaggt acgtggtgga gtcggcctgc gtctgcttga tgacccggcc gttggcgtcg
    14641 tactgattgg tgaggaagac gatggcgcgc gcgtccttga tcgtcaacat ccggttggag
    14701 gtgtcgtaag tgtactccgt cacccccgct ttggggtcag tcaccttcca gaggcgaccg
    14761 ctcgcgtcgt aggtgtagtt gacggtgcgg ccgatgttgt ctttggcctg cgtgatgcgg
    14821 ttgctggcgt cgtaggtgaa ctcgatccag cgcccgttgg aggaagtgag tttggtgacg
    14881 cgcccggtcg gggagccgaa gccgttggtg ctggcgcgcg agatgatgat cttgttgccg
    14941 tggcggtctt tgatggtgtt gagcggggcc tcgtcgccga agacgtaggt gaggccgttc
    15001 ttgagcttta agtcccaccc gttgccgttc cacttcaggc gcgaccggta gaagggagac
    15061 gaggtggtcg tgtgctcgaa ctcggcgtcg gtgaagcccg aacccggcga gatgcggacg
    15121 aagtggacgc gcccgccgtt gggcaaaatc aggtcggcct cgacgtactc ctgcgccgag
    15181 tagagataca actcgtaagg gtgcgaggtg ccaatcccga aggggcggga gttggggtct
    15241 agcgggcgat aggtgcggtt gagtttgagg ggcatcgtgt ccggcagaat gagatctgtc
    15301 ttgtcgtaca cgaagagccc tgtggcgagg tcaaccgggt cgccgtccgt gccgtccgaa
    15361 cccggattcg ggccagaacc cggcggcggc ggcatgccgt tgcggttgat catgaagccc
    15421 gtgaactcgt agatgacgac gccggggtcg ggcatcactt gccggccgtc gctcgtcacg
    15481 gttccgtgcc cgtagatgta ccagcccttg tcttcggggt cgtagttcca gaagttcatc
    15541 cgcgtgccgg ggcggtcgtt gccgtagttg ggatagacga cctgtgcgcg cggcggaatc
    15601 acccgcgacg cgcccggctg gacggtgaag aagaccggga cgttgacgcc gccggggagc
    15661 gggaacggcg tgcggtcaac cggaatcggc gtgatggtca actgcgacac cggctgcccg
    15721 tcaatgtcgc gcacgaccgc cccctgcggc acgcgcactt cgagaccggg gatgtagggc
    15781 gacgtgacgg cgacttcgcg ggaggtcggg actggcagcg tggtgacgtg ctccgtatca
    15841 aggcgcggca tccagctcgt gaacggcagc acgttcgttt tgcccgcctc cacgtccacc
    15901 ccgatcttga acacgccata aaccttctgc ggcttgctgg cggtcgcccc gtcgaggcgc
    15961 atcacgtgat gaccggcggg gaggtcggtc aggaggaagc gtcccgtgtt gtcggtgcgc
    16021 gccgagcgcg cgccgatttg gagcgtgacg ttcgcgagcg ggtggccgtt taagagcaag
    16081 acctgaccgg ctaacgccgt gacgccgggc gcggcttcga ggggcggcat cttctgccat
    16141 ggcgactcgg ggcggtggct gcgccaatcg ccgcgcatgt ttcgggtgtc gggtatccag
    16201 tcctcaccat cggcgagccg agcgtcggac gacgcgccgc gctccgtcgt gaagctcaac
    16261 atggtgtagg tcagcgcgcg gttgttctgg tcgagcgcgt cggccagcgt gatggtgtag
    16321 gtcgtctctt cgagcagctt ctcccgcggg acgatgaagg ccaacatgcc gccttccgcc
    16381 ggcgttatct tcgcctcgac tgtgccctgc ggccccttga gtgcggcggt ctgagcgttg
    16441 acggtcgtga cgctcaaggg tttcgagaaa cgcaacgcga tctgcgtctc caccggcacg
    16501 tcagtcgtcc cgtcctccgg cagcgacgcg cggaggtaag gcgcgttttc atcttcaaat
    16561 ctgttgaagc cgctaatcca cgttagctgc tcggacttgg gttcatagag cacgccgtcg
    16621 tcgaggtttg cgccgtcacg acccacgccg ccccacagca acacggcgcc gtcgggctga
    16681 agcgttgcgc tgtgcctgcg gcgcaggctt tgcagttggc cggggacggg cttgacggtt
    16741 ttcgtcagcg gctcccacaa ctccacgcgc gcctgcatct cacccttcgc cgacgtgccg
    16801 cccgcgatca ggacttgacc attggtgagc aaggtggcgg tgtgatgcgc gcgcgccgtc
    16861 agacccttga ggggcagaag ctcgaactgt ccggtgtcgg gatggaagag ttcggcgctg
    16921 tcgatcacct cttcgcctgc gcccacgccg ccgaagatca acaccgtccc gtccgggagc
    16981 agtgtcgccg agtgcgacgc acgcgcctgc tgcaagccat taccgagcgg cagcacggag
    17041 ccgtcccgag catccttcac ctgcgcgtcg cgcgaggggc cgtcggcccc ctccccgccg
    17101 agcaacagcc atctcccgtc aggcatcagc gtcgcgctct gaccgggctg aagcgtctcg
    17161 gtctgtggcg cggggactct gagaaacctg atgatgctgg cgttggcggc attggcgctg
    17221 gcacgcttca agccggcggg cggtgtttgg gcgacagctt tctgcgcgcc ggcgccggac
    17281 gcttgtaagg gcaggaggca aggccccact accatgtaca gactcagtcc gacgctcaag
    17341 acgactctca aagaaatgct ctttttcatc ccagctctcc gagtcataaa cctaatcccc
    17401 gaaagtgatc tctgaatttt cattcgcttg aagctcgtga gcgcgtgact ccggctcacc
    17461 gtgagaaact cgaactcgat ctcgaagtcg gcgcctcaag acgcgcgcgc gtgcgcgtcc
    17521 cggcgagagc cgtgcgccgg caggggtttg aagaccgcgg ccgcctagtt gccttccggc
    17581 atgagcaccg tgtcgatggc atgaatgacg ccgttcgagg tgttgatgtc agcctgcgtg
    17641 attttcgcct tgccgacttt caggccgttg ctcgtgtcga attgaagcga gttgcgcttg
    17701 agcgttttgg cgtctttcaa cttgttcagc tcggaggccg tcgccttgac cgggaagatg
    17761 tgtgcggtga gcaagtcttt caacttctct ttattctccg gccggagcaa ttcctcaagc
    17821 gtgttcgcgc tcagtttggc gaaggcttcg tccgtcggcg cgaagaccgt gaacggcccc
    17881 ccgcctttga gcgtgtccac caacccggcg gctttgacgg cctcgataaa ggtcttgaag
    17941 ttccccgcgt tttgcgccgt gaagtagatg ttctgcggcg gcggcgcctg tgccccctgc
    18001 tgcgtcgccg ccgtcgcgcc cgccccgccg ctcgcggcga ccgacagtaa aagcgccgcc
    18061 aacatcaacg ccgccgggcg cgcattaatc aaactctttc tcaacatcac taaaatctcc
    18121 ttttctcttc tgcttctctg ccgagaagca atgattgcct cttctgaagt gtcgtcgccg
    18181 tcccgccgca gggacaactc aggacgcatg ggttcggctc aggggctggt cacgttcacg
    18241 ctgacgccgc ccgtattcgc cccggaagga tcaatgcgaa tgatgaaggc gcctgtcgtc
    18301 ggcagcgtct gcggggcgag gtcgaggttg ccgccatacg acgtggctga cgtgataagc
    18361 attccgtccg gggtcagcag actgacgacg acggtgctca tcgtgttgct cgtgaggtga
    18421 accgttatgg cttgcccctc gacgccgtcg aaagtgactt tcgcgttctg gcccggcgcg
    18481 gtcgtcgtca ccgtggccgc cgcgccgccc ggcgtaatcg tgcccgcgtt gacatcgctg
    18541 gcgtcgttaa gctgcaacgt catgctgccc gcgttggcgc cttccgggtc aacgaggagg
    18601 gtgaatgtgc cgttggcgtt gagcgtctgc gcgtcgatga agccggcgcc gttgaggtag
    18661 acgtaggtgt tcgtgacctg tgagccgtcg ggccgcagca ggtagacgta ggcgtaattc
    18721 gggatggtgc tgccgctgat gctcagactc aggcgttgcc cgtagacgcc gctgaaggtc
    18781 agtctcgggt tgtggccggg ggcggcggcc gtgacggtga cgggcgtgcc cggcgtgatc
    18841 ccaccgctcg cgtcggcggg cacgtcgaag agcgtcagcg ttgcgctgcc ggtgttggtg
    18901 ctcgaagggt ctacgaagat cgtgtatgtg ccggttgttg gcagcgtctg cgtgtcgagg
    18961 aatgcgccgc ccgtgcttga gtagacgtat gcgagcgtcg ccccgtcggg gttcttcagg
    19021 tagacgtatg tgccggggat ggtgacggcg gagatgcgta gactgacgcg ctgcccttcc
    19081 gtgccgttga aggtcaagct ggcattctgc ccggcgctgg tggtggtgac ggtgacgggc
    19141 gcgcccccgg tcgtgatggc gccgccgaca tcgctcacgt cgttgagctg cacggtcagg
    19201 ttgccggtgc ccgcgccgtt cgggtcaacg cggatcatgt acgttcctga gatcgggagc
    19261 gattgggcgt cgatgagcgc ggagccgttc tgatagacgt aggtgttggc gacctgcgag
    19321 ccgtcgggct tgagcaggta gacgtaactg tagttgggaa tcgtcgcgcc gctcacgctg
    19381 atgctgaggc gctgcccgta gacgccgttg aaggtcagtt gcggattgtg gccgggggcg
    19441 gcggccgtga ccgtgacggg cgtgcccggc gtaatcgtgc cgctcgcgtc ggcgggcacg
    19501 ctgtagagtt ggagcgtcgc gctgccagtg gccgtgccct gcgggtcaac cagcagggtg
    19561 taagttccgt ttgcgggcag cgcttgcgtg tcgaggaacg cgccgcccgt acccgtgtag
    19621 acgttggcga gcgacacgcc gtcgggggca agcaggtaaa cgtagctgcc ggagatagac
    19681 acgccgctga tgttcaggct cacgcgctgt ccggccgtgc cgctgaaggt gagtctggcc
    19741 ttctgcccgg cggcggtcgt cgtcgccgtc acggccgagc cgcccggtgt aatcgtgccc
    19801 gtggaaacct ccgcggcgtc atgcaactgc aaactcatcg cgcccgcgcc cgcgccctcc
    19861 gggttaacga ggatggtgta cgtccccgac acaggcagcg tttgcgtgtc gatgaagccg
    19921 gtgccgttca ggtagacgta ggtgttcgtg atctgcgacc cgtcgggtcg cagcaggtag
    19981 atgtaagcgt agttcgggat ggtgctgctg ttgatgctca gactcacgcg ctgcccgtag
    20041 acgccgttga aggtgagacg cgcgttctgc ccggcggcgg tggtggtcgt cgtgacggcc
    20101 gcgccgcccg gcgtgatggt tccgctggga tcggcaggca cgttatggag cgtcagggtg
    20161 atgctgcccg tgtatgtgcc ctgcggatcg acgagaatcg tgtacgagcc gttaccgggc
    20221 aaagtctgca cgtcgaggaa tgcgccgccc gtgcccgtgt agacgtaagc gagcgtggct
    20281 ccatcggggg ctttcagata aacatagctg ccgggaatcg tcacgcccga cacattgagg
    20341 ctcacgcgtt gtccggccgt gccgctgaag gtcgccacgg agttcttgcc cggtgtggcg
    20401 acggaggtcg tcacagccgc gccgcccggc gtaatcgtgc ccgcattaac ctctgtggtg
    20461 tcgttaagct gcaaggtcat gctgcccgtg ttggagcctt cggggtcaac gaggagcgtg
    20521 aacgtgccgg tggcattgag catctgcgtg tcgatgaagc cggtgccgtt gaggtagacg
    20581 taggtgttcg tgacctgtga gccgtcgggc cggagcaggt agatgtaggc gtagttcgtg
    20641 atgctgctgc tgctgatgct gacgctcacg cgctgcccgt agacgccgct gaacgtcaac
    20701 cgcgcgttct gtccggcggt ggtcgtcgtc accgtcacgg ccgaaccgcc cggcgtgatc
    20761 gtgccggtca catcgctgac cggcggggtg acggttacta ttccgccgtc agttaaaccg
    20821 accgtcatgc tgccggtgtt ggttccctgc gggtcaacca gaatcgtgta cgtccccgcc
    20881 gactgaagcg tctgcgtagc gatggacgcg gtgccgttct ggtagacgta attgttagta
    20941 atctgcgagc cgtcgggccg gagcaggtag acgtagctgt agctcgggat ggtgacgctg
    21001 ctcagattaa cgctcacgcg ctgcccggcg acggcggtga atgtcagccg gggattatga
    21061 ccgggcgcgg cggccgtgac cgtgaccgtg gagccgccgg gcgtaatcgt tcccgcgttg
    21121 tcgggcggca cgttgtagag ggtcagggtg gcgctgccgg tgttggtgcc ctgcggatca
    21181 acgagaatcg tgtacgttcc attggactgg agcgtttgcg tatccatgaa cgtcccgccc
    21241 gtgcccgtgt acacgttggc cacactcgtc ccgtcgggat tcaggagata cacataagtg
    21301 ccgggtatcg tcacgcccgt gacgctcaag ctgactctct gtccggtcgt gccgctgaag
    21361 gtgacgcgcg cgctttgtcc ggcggcggtc gtgctggccg tcacggacgc gccgtcaggc
    21421 gtaatcgtgc cggcgttggc atcggtcgca tcattgagct ggagggtcat gctgcccgtg
    21481 ttcaccccct gcggatcgac gagcaccgtg taggttcccg tcaacggaag cgactgcgta
    21541 tcgatgaggc cgacgccgtt ttgattgatg taggtgttgg tgacctgcga gccgtcgggc
    21601 ttgagcagat agacgtagct gtaactcggg atcgtcacgc cgctcaagct gatgctcacg
    21661 cgctgcccgt aggcgccgct gaacgtcaac ttggagttct gtcccggcac gcccatcgtc
    21721 accgttgtgg gcgcggcgcc cggcgtgagt gtgccgctca cgtcggcggg cacgtcgtag
    21781 agcgtcaggg tcgcgttgcc tgtattaccg ccggtcgggt caacgatgat gctgtaagtt
    21841 ccagaggtcg gtagcgtgcg cggttcaacg aacacgccgc cggtgccgga gtaggtggcg
    21901 gcgacgagcg tgctgccgtc ggggttgagg atggagacgt agctgccggg tatggtcacg
    21961 cccgtcagat tcaagctcac ccgctgtcct tccgtgccgc tgaaggtcag tctggcgttt
    22021 tgtccggcga cattgggcga caccgtcacg gccgaaccgc ccggcgtaat tgtgcccgcg
    22081 gcaatgtcgg aggcatcgtt gagctgaagg gtcatgctgc cggtgttcgc accttgcggg
    22141 tcaaccatga tggtgtacgt ccccgacacg ggcagcgtct gcacgtcgat gaagccggcg
    22201 ccgttctggt tgacataggt gttggtaact tgcgacccgt cgggcttgag caaatagatg
    22261 taggcgtaac tcgggatggt gctgctgctg atgctgatgc tgacgcgctg cccgtaagtg
    22321 ccggtgaacg tcagtcgcgg gttgtgaccg ggcgcggcgg ccgtgaccgt cacgggcgcg
    22381 ccggacgcga tggttccgac cgcgtcggcg ggcacttcgt agagcgtcag cgttgcgctg
    22441 cccgtatgtg tgctctgcgg gtcaacgagg atggtgtagg ttccggtgac gggcaaggtg
    22501 cgggcgtcga ggaaagtgcc gcccgtgccg aagtgcgtat tggcgaccag agccgagccg
    22561 tccgggctga taatcgagac gtaactgctg gggatggtga cgcctgtaac actcagactg
    22621 actctctgcc cttccgtgcc gttgaaagtt actttggcat tctgcccggc gctggtggtc
    22681 gtgaccgtca cgggcgcgcc ggccggggta atcgtcgccg tcacgtcgct cagatcgttg
    22741 agctggaggg tcatgctgcc ggtgttcgcc gcctgcgggt caaccatgat ggtgtacgtc
    22801 cccggcgttg acagcacctg cacgtctatc aggccggcgc cgttttgata gacgtaggtg
    22861 ttcgtcacct gcgacccgtc gggccggagc aggtagatgt aggcgtagct cgggatggtg
    22921 ctgctgctga tgctgatgct cacgcgctgc ccgtaagtgc cggtgaacgt cagtcgcggg
    22981 ttgtggccgg gggcggcggc cgtgaccgtc acgggtgttc ccgccgtgat tgttcccacg
    23041 ccgtcggcgg gcacttcata gagtgtcagg gtcgcgttgc ccgtgtaacc gccggtcggg
    23101 tcaatcagaa tcgtgtaggt tccggtgacg ggcagcgtgc gcgggtcgag gaacgtgccg
    23161 ccggcgccga agtgagcggc ggcgacgagc gcactcccat cagggttgag gatggagacg
    23221 tagctgccgg gtatggtcac gcccgtcagg cttaagctca cgcgctgtcc ttccgtgccg
    23281 tcgaaggtga ctctggcgtt ttgtccggcg acggtggtgg tcacggtggc gggcgcgccg
    23341 ccgggcgtaa tggtcgccgt cacgtcgttc actgcattta attgcagagt catgctgccg
    23401 acgttggccg cctgcgggtc aacgaggatg gtatacgtgc cggacatcgg cagcacttgc
    23461 gtatcgatga ggccggcgcc gttttgatag acgtaggtgt tcgtcacctg tgagccgtcg
    23521 ggccggagca ggtagatgta ggcgtaactc gggatggtgc tgctgctgat gctgatgctc
    23581 acgcgctgcc cgtagacgcc ggtgaaggtg agcgtggcat tctgtccggg gacggtggtc
    23641 gtcaccgtga cgggcgcgcc gccgggcgta atcggcacat tgccgtcggc cggtacgtcg
    23701 tagagtctca gggttgcgct gcccgtgtag gtgttcgacg gatcgacgag gatggtgtaa
    23761 gtgccgctgg cggggagcgt ctgcctgtcc atgaacgctc cgcccgtgcc cgaatagaca
    23821 taggcgagcg tggttccgtc cggtttctga acgtagacgt aagttccggg gatagtgacc
    23881 gcgctgatgt cgagactgat tttttgcccc gccgtgccgc tgaaagtcac ctgggcgttc
    23941 tgaccgggaa cagtggtggt cgtcgtcacg gccgcgccgc cgggtgtaat cgtgccgccg
    24001 ttaacattcg tcgcgtcgtt cacccgcagc gtcagactcc cggtgttgga gccttccggg
    24061 tcaacaatga gggtgtaggt tccggtcgcg ggcactgtca atgcgtcgat gaagccggcg
    24121 ccgttctggt agacatacgt gttggtgagc tgcgtgttgt ccggcttata aatatagacg
    24181 tatgtataac cggggatgtt ggaactctgg atgtcgagac tgatcctctg cccggcagtt
    24241 gcgtcgaaca gaagcatcgc cactttgctc gcggtgttga cggagacggt ttttacttct
    24301 cctaacgcga tgcgccccgt ggactcgaca tccgttcccg tatacggaga tggaggaata
    24361 taaaagtcgt tactactggt ggccttcccg tcaggggtcg tcaccgtgat gcgccccgac
    24421 gacgctcccg tcggcacagt cgccgcgatg ctcgtcgccg ccgccgaagt gacggacgcg
    24481 cgaacaccgt tgaaggccac tctgttgctc gaagggacgg tctggaaatt ggtgcccgtg
    24541 atcgtcacgg cggcgccggg agagccgacg ctcggagtga agctggtaat cgtcggcagg
    24601 ccgggatcgc cgacgatgaa agtggcgctg ctggtggccg taccggaggg cgtggtgacg
    24661 ttgatcgtcc ccgtcgtggc tccggcaggc acgttggtca cgatttgtgt cgcggttgag
    24721 gaggcgacgg ccgccacgac gccgttgaat ttaaccgtgt tctgagtggg cgtgctgctg
    24781 aagcctgagc cgtagatgat gacctgcttg ccgaccgcgc cgctgttcgg tgtgaactcg
    24841 ataatggatg tttgggacgc gctctggcgt gagatggagg tgagattgcc gaccgcatcg
    24901 taagcatagg tcacccgctc gccgttcggg tcaaccacgc cgacgagcct ccccagctca
    24961 tcatagatat atgtgatttg agtggaattg agcgcgaagc tgaccgtcgc gggtgttggg
    25021 gcggagacct gttgcccgct ttggctctga gtctcgtaac ccgccgccga cgcctccacg
    25081 tcatacgtcc cgactaaaag atttgtaatc gaatagctgc cgtcgccgcc gctcgtcgcg
    25141 gttcccttca ccgcgcccgt ttgaatggct ttgacctgtg ccccgacaat cggcgcgccg
    25201 tcgattgcgc gcgcgacact cccggagatt ccactgatct cgcccacgct gaaggtgctg
    25261 ctgttgctcg ccgcgccgtt gaccgtcacg acgaccgggc cggtcgtcgc ccccgaggga
    25321 accggggcca caatcgtgct ggcgctccaa ctgccgggag ccgcggcgac accgttgaag
    25381 gtgatggtgc tcgtgccttg cgtagcgccg aagttggagc cgctgacggt gacgctcgtg
    25441 ccggtggggc cggcggatgg tgaaattgtg tctaccgtcg ggcgaatggg tagagggaca
    25501 gtgaccaaag aattgtagtt accgttgagc gttccctcga tgtcgagttc cgcggttgta
    25561 ttggtggtgg gcgcggcggt gagattgacc ccgacccaga cataaaacct gtcggcggcg
    25621 gtcaccgaaa tattagtgct cgtggtagta gtgaaagtgt atttggtgag cgtcgaggtg
    25681 agtgctgtcg cactggtggc ggttccgagt agcgttccgg ccacgccgtt caaacgcaat
    25741 ttggcgcgcg gataaatcgt tccgccggtg gtggtcttcc tcatccagag ggtgaaactg
    25801 atgtttgagc cggcgcttat cacaccggaa ctattcggca cgccgctcgg ggtgtcgaag
    25861 gctttgacga gatactcgcc cgccgccacg cccttcaggt tggcggactg aacggcgaga
    25921 ctcgtgccgt caggccccgc ggttttgagc tggaaaagac cggtcgtcgc cgacgcctct
    25981 ttatgaaggt gataggtggc cgtctgagct tgcgctgaca gcgccagcac cagcagcaaa
    26041 ataaaagttg aaagaagctt actcccggtg cgcgccatga actggccctc cacagtcgcc
    26101 gagtcgaaac tgccgttgct tgtgcctcgt gtctctacag tcatcgctct ttggtcggta
    26161 aagaaaccgc ctcgctcgat gcgctggcag acgctggcgt gtgttgaaga gatcatcagt
    26221 tatttactga gttcggtgaa gagccgaaat tatcaacaaa gaaacgaccg gaaactttca
    26281 gcgaacaact ctttaacgat aggcagagca ccgccccgct cgctcgatgt gtgcgaagtt
    26341 gaactttcgg cacttagcgg cgagttactt aaattagtgt cggctgattg ttttgagtct
    26401 tacgtactac aggcggttat caacaaacct gtgagtgtta ccttgtcgtc cctctgcgat
    26461 gcaaacgggc ggcgcgtcat cgaaaagcgt agccaccgtc ctgcacagcc caagcaaata
    26521 gcgcgaaata tctgcgccat tgattcatta aggtgaagtt tgaaggctaa tgagatcgag
    26581 gtgagaggag gctctcgccg gggacaagtt gccaatgcgt gcgcatagtg attgaacaga
    26641 atatttttgt caagaactat ttgcaaaagt tttgctgaaa ttttttttcg gcgtcgatta
    26701 actatttttt aaagtttctg gctgtgttgc aaacagtgat gagaggcgag aagagtctcg
    26761 gtggggactt ccaccaagct gagacatcgc gaagtcttcg tgtgcctcaa ctcgattttt
    26821 gtaacacagc ctgtccgagg agaaggtctg cgagggcttc ggagatgggg agaccggggg
    26881 tgcgttcgag ccagaagaat tcgccggagg ggttgagttc gaggaagacg tgccgagcgt
    26941 cgggcgtgag gatgatgtcg atagcgccgt agttcaggga aaagtagtcc atgaggcgca
    27001 ggattttttg ttcgacttca agggggagtt ggtagggtcg ccagtcctga agcatgcgga
    27061 ggccgtcgcg tcgccagtcg tgagcggcgc gggctgaaga ctgtgagtcg agggaggcgg
    27121 acatgacgcg tcggccgacg accgtgacac gaagctcaag cgacttgggg agtaactcct
    27181 gaaaggttgc ggggcagagg ctcagaccgg agaggtctgc gaggtcttcg ggcttcacgg
    27241 ggttggtgaa aacgacgagt tctcttccct cgtcgtagac cgcgaaggag gagagcatct
    27301 tcgtcaccat cccaccctcg cagctcttgg cgaaggcgcg gacggccgcc gggtcgttcg
    27361 tcgtcagcgt gcgcgggatg tcgagtccga gatcgcgcgc cgcctgtagc tggagctgct
    27421 tgttctcggc gtgacggatg tggcgcacct cgtccacgcg gaaggctttg aggctggcga
    27481 gcatgccgtg ggcggcggca ctcgcttcgc ccaaggacgc gtggcgcagt tgcccgtcga
    27541 gcgtcgccgg gagacgcgcg ccgaagttga ggcggcggtg ccagacggcc gtgacctcgc
    27601 gcagatcgaa ctcacccgct tcgttcgtca gcgtcagacg ctcgtcgccc gcggcgccgt
    27661 aataggcgga gaggcgcacc tcggtcgggt agcggtcggt gtcgaagcgg atcgcgtgcc
    27721 cgcccctgcg cgcgacggcc tcggccacca acgcgacgca ctcgttgtcg tcgctgcgtg
    27781 tgatgatgag aatggacata agcgtcagag gtcagaggtt agaggttaga ggtcagaggt
    27841 cagcagcgct ccgcgtcgat gatattgacg ccgacacacg gacgatcaaa ttaagagacg
    27901 gtccttcttc tgacctctga cctctgacct ctgacctcta gtagtgattc ggcgatggcc
    27961 tccgagatgg gaaggccgag gtcgcgttcg agcatgcccc attcgccgga gggattgact
    28021 tcgaggaaga cgtgtccgcc cgaaggggtg tggattaaat cgacggcgcc gaagacgagg
    28081 ccgagttcag acatcaatgc ccgcaggccg cttgagactt cggcggggag ttgtgccttc
    28141 tgccagcgac attcgccggg tgcggcgcgc cgccagtctg tctgagcgcg cgacgtgccg
    28201 ctcgcgtcaa gcgcgcccgc caggctacgg cccgccacac agacgacgcg caactcgcgc
    28261 gctttcggaa tcaactcttg aaagaccatc gggctatggc gaagtgtctc ggcgcaggcc
    28321 aggtcttctt cgctgacgcg gctcgtgtag acgaagggct gcgcagcgtc catgccgacg
    28381 gtgagcgggc ggaggagttt cgccaccgtc cggccttcgg tctcggcgaa gaactgtcgg
    28441 gccgcggccg cgtcgttggt gacgagggtg cgcgggacgc gcaggccggc gcgcgcggcc
    28501 agtcggagtt ggcgctgctt gttctcggcg tcgcgctcgc gctgaaggtc gttgacccag
    28561 cgtgcgtcgt ggagcgcgtc gagaaagcct tcgagcgcgg cgaccgactc cctgacgcac
    28621 attaagcgat aacgctcgtc gaggtcgtcg gccatgcgcg gcgaccagag cttgcgcgcc
    28681 cagacggcgc gcacctccgc ggcggacacc tgctcgcccg cgtccgtgta gagatgtgcg
    28741 gcgcgtgcgt cgcccgcgcg ggccgagagc ttgacggatg acgggaagag gtctgtgttg
    28801 aagcgaacgg ggcgggcgcc cctgcgcgcg agagcctgcg cgacgagatc gacggtgtag
    28861 aaatcgccgc tgtgcgtcag cagcaggacg gcgtcgcgcg gtgttgtcgg aatcatgcgt
    28921 cgaaggcgag tagttcaagg gtgcggcccg cgtccggcga gcaccgcgcg gcggcaggat
    28981 agctctcgcc gcgaaggctt cggacgaggg ccggcgccct cttgcgtttg gagggccggg
    29041 cggacgagga gacgaaaggc gctcgacgcc cgaccggctt ttcagcgacc gactgaaacg
    29101 gtcttcttat ttatgcggcg ctacggtctc gttgttgcca gacgcgtact caagtcgttg
    29161 gtcgatctcg tccctgtcgg acgggtactt gagcgtcatg gcgcaaacct ccggggtgtc
    29221 ctggctttcg aggaaacgcg cgaagaatgg caggctccgc tcggagcctt cgggggcgcg
    29281 tgcttctgac tggtctcgca ttgagtgatc tccttcaggg gttaaagtga gctgctggcg
    29341 aggtacgaaa aatctgacga cggcagctat gatgggttgc cagcgctttg gcgtttactc
    29401 ggggttcgat taattctcga agaccggagc ggcgcttgta tataattcgg taattatccc
    29461 gatgattgtg ttgcgaaaat tggtttgaag aaaagaacac cgcgagaact ttttttttac
    29521 aattccgctg ttgacccgcc gtccgactct tcggaagata tagacactca tgatttgaga
    29581 aggcgatagc aattatcaat tgagtgtatg gcaagcataa ctcaccgccg agagtgaaat
    29641 tagattcgac caatgagccg tttcgcatga agaatctttc gcgccgcctt tcgccactcg
    29701 ccgccttcgt gttcctgctg tcggccgtcg cgtcggcgcg cgcgcagtca cccgagctga
    29761 tggacgcggc cgagacgcag ctcgcgctgc ggaggctggg cgtcgtgggg agtgcgctct
    29821 acgtcggcgc gcacccggac gacgagaaca cggcgctctt ggcctacctg gcgaacgagc
    29881 gcggcgtgcg cgcggcctac ctgtcggtga cgcgcggcga cggcgggcag aacctcatcg
    29941 ggacggagca gggggcggcg ctcggcctcg tccgcacgca ggagcttctg gcggcgcggc
    30001 gcatcgacgg cgcggagcag ttcttcacgc gcgccatcga cttcggcttc tcgaagtcgc
    30061 ccgaggagac gctccgagtg tggggccgcg aggcggcgct tgcggacgtg gtctgggtca
    30121 tccggcgttt ccgccccgac gtggtcatca cgcgcttccc cacgacgggc gagggcgggc
    30181 acggccatca caccgcctcg gccatcctcg cgcgcgaggc gttcgacgcc gcgggcgacc
    30241 ccgcgcgttt ccccgaacaa ctcgacttcg tcaagccctg gaagccgaag cgcctgctct
    30301 ggaacgcctt cgtgcggcag ggcgagcggc ccgctaacgc cgacaaactt ttgagcgtgg
    30361 acgttgggaa gtacaatccg ttgctcggca agtcctacac cgagatcgcc gcccgtagcc
    30421 gctccatgca caagagtcag ggcatgggcg cggccgaacg gcgcggcacg gccctcaact
    30481 acttcgcgca cgtcgcgggc gagccggcgt cgaaagacat cttcgacggc gtcgatttaa
    30541 cctggcggcg cgtcgcgggc ggcgaggccg tgggacagtt gctcgaacag gcggagcgcg
    30601 agtttcaagc gtcgaacccg cagggcttac tgccccacct cttccgcgcg cgcgccgaga
    30661 tgcgaaagct gccgacgggc gatgcgctcg tcaatgcgaa gagcaatcaa ctcttcaaag
    30721 taattctcaa cagtctggga ctgtgggccg aggccgtcgc cgccgagcct tacgcgactc
    30781 cgggcgctga cgtgcgcgtc gccgtcacgc tcgtcaatcg ttctgacgtg cccgcccgtt
    30841 tcgagagcct cgcgctcttc agcggccaga cgcatgcggt gaacaaagag ttgaagaaca
    30901 accagccggt gacgacggaa gtgctcgtgc cggcgcccgc cgaatacagc cagccctact
    30961 ggcttcggaa agagccgggc aaggggctgt tcacggttgc cgagcaggag agcatcggcg
    31021 gcgccgagcc gacgccgcac atcccgctgg tggtgaggac cacgaccggc gatggcgaga
    31081 agatcaactt cgcgctgccc gtcctctttc gctggactga ccgcgtgcag ggggagatgt
    31141 accggcccgt agtcgtcgtc ccggaggtgg ccgtgcgtgt ggaggagaag gtgctcgtct
    31201 tccccgaccg aaggccgaag caggtgcgcg tgcgtctgaa gagcaacacg gcggcggccg
    31261 tctccggcac gttgcgtctg cgtctgccgg ccgggtggac ggccgcgccg gccgaggtta
    31321 cagtcgcgct caagtcgaag ggcgaggagt tcgcggccga gttcgccgtg acgccgcccg
    31381 ccgccgccgc gacggccacg ctcgcggccg agttcgagac gaatggtaag aagttcacgc
    31441 gcgctctggt cgagatcgac tacccgcaca tcccacggca gacgcttttg cccgcggccg
    31501 agacgcgcct cgtgcgcgtt gacctcgcgc ggcgtggcgc gcgcgtcggc tacgtgatgg
    31561 gggcgggcga cgaggtgccg gaggcgttgc ggcaggtggg ctacgatgtc gtgctcctct
    31621 cggacgaaga cctcgaaggc gcagacctct cgaagttcga cgcggtcgtc acgggcgtgc
    31681 gcgcctacaa cacgcgcgcg gcgctgcgcc ggcagcagcg ccgcctgctc gaatacgtcg
    31741 agcgcggcgg cacgctcgtc gtgcagtaca acacggcgga gcgcgcgctc gaaggcttcg
    31801 cgctcggccc ctaccccgtg aagctttcgc aggagcgcgt cgcggtcgag gacgcgccgg
    31861 tcacgctcgt cgcgccgcgc gacgagatgc tcgacgcccc gaacaaaatc actgcggccg
    31921 atttcgacgg ctgggtgcag gagcgcggcc tcaacttccc ctcggagtgg gacgcgcgtt
    31981 accgcacgcc cttcgagacg cacgaccccg gcgaaacagt gaagcccggc ggcacgctca
    32041 tcgcgcgtca cggccgaggc gtctacgtct acacttctta ctcgtggttc cgacaactgc
    32101 ccgccggggt gccgggcgcg taccgcgtgt tcgtcaattt aatctcggcg ggcaaacaac
    32161 acagtaaggc ggaagcccaa ccgtgaggga gggcgtttct aaggtgggcg gtgctgacgc
    32221 tgacgtaact ggcacgccct ctctcacggt cgggcttccc cctcgaatgt cctgctgaaa
    32281 atgcctgaca caacgaacca acacagagac tccggcgacg agcggccccc gttcggcggg
    32341 agttgggccg tgctctatgc ggccgtgctg ctgaacctcg ccctgctcgt cgtgctcttt
    32401 tatctcttca cgcgggcctt ccgatgagac cactcgactg gctggtgctc gccgcggcgc
    32461 tcgtcggcat cgtcctctac gggctgtgga agggacgccg gacgcgcgac ctcgacgact
    32521 tcctcgtcgc cgaccggcgc atgcgctggc accacgtcgc cctctccatc atggccacgc
    32581 aggcgtcggc catcaccttc ctctccacgc cgggtcaggc ttacacggac ggcatgcgct
    32641 tcgtgcagtt ctatctgggg ctgcccgtgg cgatggtcat cctctccgtc acggccgtcc
    32701 ccatctatca ccggctgaag gtctacacgg cttacgaata cctcgaaggc cgcttcgacc
    32761 tgaagacgcg cacgctcgcc gccctcctgt tcctcgccct gcgcggcctc tcgacgggcg
    32821 tctccgtcta cgcgccctcg ctgatcctct ccgtcatcat gggctgggac atccgtctgg
    32881 tgatctttct catcggcggg atggtcttgc tctacgcggc gcggggcggg gcgcgcgccg
    32941 tggacaacac gaactttcag cagttcctca tcatcatggg cgggatgatc gtcgccttct
    33001 tcacgctcgt ctcgctgctc cccgcggacg tttctttcgt cgaggccgta cacgtcgccg
    33061 gccgattggg acgcctcaac gcgattgact tctcgttcga cccgtcgaac cagtacaact
    33121 tctggtcggg actcctcggc gggatgttcg tcgcgctcgc ctacttcggc accgaccaat
    33181 cgcaggtgca gagatacctc acaggcgagt ccgtcacgca gagccgtctg ggcctgctct
    33241 tcaacgggct ggtcaaggtg ccgatgcagt tcttcatcct tttcctcggc gcgatggtct
    33301 tcgccttcta tcagttcacc gagccgccga ttttcttcaa cccggtcgag gtggagcggg
    33361 cgcgcgcgag tgtgcgcgga gcgcagttca gcgcgctcga agcggagtac tcggcggcct
    33421 tcgcggcgaa ggagacggag gcgcgcggcc tcatcggggc gatgcgggtg ggcgacgagg
    33481 cagcggaagc cgcggcggcc gagcgtctgc gcgcggcgca gggacgcgcg ggcgaggtgc
    33541 gcgccgcggc cgtcgagata ttgaaggaac agaacccgaa gccgaacgat acgaactatg
    33601 tctttctgag tttcgtcatc cactacatgc cggccggggt cgtcggtctc gtcttcgccg
    33661 tggtcttctt cgcctcgatg acttcgaccg cctccgaatg gagcgcgctc gcctcgacga
    33721 cggtcgtgga catccagcgg cgactcgtca ggcgcggcgc gagcgaccgc tattatctcc
    33781 tgtcgtcaag gctggcgacc ctcttctggg gactcttcgc cgtggccttc gcgcagtacg
    33841 ccggacacct cggctcgctc atcgaggccg tcaaccgcct cggctcgctc ttctacggca
    33901 cgctgctcgg catcttcctg ctcgccttct acttcaaacg cacgaatggc acggcggcct
    33961 tcaccggcgc actcgtcggc gagggcgtcg tgctctattg cttcttcttc acgaagatcg
    34021 ccttcctctg gtacaacgtc atcggcgcct tcaccgtcat cgccacggcg ctcgtcgtca
    34081 acagagcagt aggcagtaag cagtaggttg tcggcggccg acgagccgcg gagcggcgga
    34141 agattgtagc cacgggcgca agcccgtgga acagtcgcca gaagaatttg aaaagccccg
    34201 taggggcgaa agaacatcaa cgtgcatcac gctcaacgtc gatgttcttt cgcccctacg
    34261 gggcttttgt ttctttacct gcgctgaccc cacggcttac gccgtgggct acaatctgcc
    34321 gccgcctccg gcggctggtt gtcgtcttac agcttactgc tcactgccta cttcctatta
    34381 cttcttcgtc ttccactcgg cgacgacctt ctcgaaatta gccatcgcct gcgcgttggc
    34441 gttggcggcc ttgccgacgg cgagcgcctt ctcgccggcg gcgatggcgt cggcggtctt
    34501 gccctgcgcg gcgaggatgc gcgcccggag attcaggttg ttgaagttct ctttggtctt
    34561 gacggactgc tcggcccagc gcatcgcctc gtcgaggtcg agcttgttct ggagggcgta
    34621 gttcgcggcc tgaaggggcg tctgccagtc gtcgggcttg gccgcggcaa tcgcggcccg
    34681 cgctttcacc agagtgagcg cctttacgtc cttgacctcg accgtgaagg gcaccgtcag
    34741 cttctcccag cggatgttca cacgcgcgga ggtctccccc tcgggttcga tgctgtaggc
    34801 gagccactcc tgatttacgg ccgccgtctc cggcttcgtc ttgacgcgga gcgcgtcctt
    34861 cgccgcgtcg taggtgaagc tcccccactg gccgtcgtcc ttgttgaaga tgatggtcca
    34921 ctcagcgggg ccggggatgg tgtgcagact gtagctgccg gcggcgagcg gctgcccgtt
    34981 aatcagcacg tcgtcggtga ctttgaagag cgtggcctcg ttggcgcccg cgcgccagac
    35041 gtggccgtag gggacgatga cggcgtcctt cgggcgcgtc tgctggttgt cgagcgtcgc
    35101 ctcgccggcg gcgctcccgg ccggcgcgtc gccccagatt ttgcggctct tcacgccggg
    35161 ccggctgtag gtgatgctca cgtcggtgac gccgaccgtc tgcatcacgc tcgccttcgg
    35221 gctggggcgc ggcaggcgca ggggcgactg cgcggagacg agcggagcgg cggtcaaaag
    35281 caaaagcgac agggcggcgg cgcggcggaa aaacttctga gcgggtttca tcttaattct
    35341 cctcgtgcgt caaggggtga gtcgtgctgt tcggaaatgt cacggcttgc ggcggatggt
    35401 aacaaaaaac cgcgcgtgaa atactcacac agttgtaaaa agtggcggct ttgtccgctc
    35461 ggcattaccc ggcgcgcccg acgaggctgg cggccacggc catgagttcg gccatctcca
    35521 cgggcttggg cacgtgcatc tggtagcctg ctctgagcgc ctgaagtcga tcctccgttc
    35581 gggcatacgc cgtcagggcg atggccggca cccgccctcc attctcggcg ggaagctcgc
    35641 gcacgcgccg gatcagttcg tagccgtcct cgcccggcat tccgatgtcg ctgatcaaca
    35701 gatcgggcct ttcattttcg atggcttcga gcgcctcttg aactgagctg gccgtcctca
    35761 cttccgcgcc acaatggccg atgcccactc tgagtaattc ccgcgcgtcc agctcgtcat
    35821 ccaccacgag caccttcaac ccgtccaacc tctcagggca atcgtatgaa ggaagcgtat
    35881 ctctgctcgc cggatgcacg cgctcatcca caccagtatt ccggtagacg ggggcgaccg
    35941 gcagcagcac cgtgaaggtc gcgccctgcc cctcgccggg actctcggca cgcacggcgc
    36001 cgccgtggag ttcgaccaga tggcgcacga tggcgaggcc gaggcccagc ccgccgtgtt
    36061 gacgactggt gcgctggtcg gcctgccgga agcggtcgaa gacgtagggc agaaactccg
    36121 gcgcgatgcc cgccccgttg tcgctgacgg cgatctcgac gtgcgagttg acgcgctcga
    36181 gcctgacctg cacccgcccg cctttcggtg tgaacttgat ggcgttcgcg agcaggttcc
    36241 acactatctg ctggagcctg acgggatcgc ccgagaccga gtccacaccc gtgtccatga
    36301 tcttctgcac gcgcacgccc ttggcctccg ccgcgggcat caacgcctcg atggccggct
    36361 cgatgaagga gttcgggtcg atggagcgca cgtccagacg gagcttgccc gtgatgatgc
    36421 gcgaaacgtc gaggaggtcg tcgatgagtt gcgcctgcgt ccgcgcgttc cgctcgatgg
    36481 tctccaaagc cttgtccgcg ctcgcgcccg cgaagtgtcc cgtccgcagc atgtgcgccc
    36541 accccatgat ggcggtcaaa ggcgtccgca gctcgtgcga gacggtggcg aggaactcgt
    36601 ccttcagacg gttcgcttcc cgcagctctt cggccgcgcg ccgctgctcg tcaacgtccg
    36661 tgttcgtccc gaaccagcgc acgatgcggc cctgagaatt gcggagcggg ttaacgcgcg
    36721 tcaggaacca gcggaacacg ccgtccgcgc ctctcagcgg gaactccatc tcgaacagct
    36781 cgcccgtctc gattgacgcg cgccaacgct ccaacacttt gggcagcact tcggggtcgt
    36841 gtacggactg ccagccccag ccctccatct gctcgggcgt cgttccggtg tagtcgtacc
    36901 agcgctggtt gtaccagaaa atataaccgt cggcatcggc catccacacc agttgcggca
    36961 cggcgtcggc gagcgtgcgt aactgactct cactctcgcg cagcgcctct tcggcctgct
    37021 tgcggtcaga gatgtcgagc gacaggtaca gcactgatcc ttcccggccg tctcggcccg
    37081 gcattgccgt ccacgtcacc agaagttgcc gccgcccctt cgggagaacg acgtctatct
    37141 cctgtgtctc gctgaggccg gtggtgtaca cgcggtcgag aatcggggcc actactttgt
    37201 cggcttcagg cacgacttcg gcgtgcgccc gccccgtgaa ctgctcttcc gagagtcccg
    37261 tgagttcgca cgtctcccga ttgaccagca ggaagcggtg ggccgggcct tccatgacgc
    37321 cgatggcgag cggggcgtgc tccaacacga ggcgggtgaa gtctcgctgt cgctcggcct
    37381 cggcacgggc cgactgttcc cgcgacagca gatcctcaag caggcttctc gactcccgca
    37441 gctccacttc ggcctgcttg gcttccgtaa cgtcgcgcgt aaagcagcgc gtgtggatga
    37501 acttgtcttc ctcccacatc acgttggagt tgatgagcac gtcccgtatc gagccatcct
    37561 tacacttgag gcgcgcctcg tagttatgaa gcgtctcctt attaaccagt ctggtcagga
    37621 tgtcattgat cacggccgca tcggcatgga actcggcgat gtggcgaccg acgtactctt
    37681 cgcgcgtgta gccgagcagg tctaactcag cctgattggc ccacaggatg cggccgtcgg
    37741 ccccgaccca gtgcaggccg acggaagcgt tctcgatgaa gtctgacaac tcacgctcgc
    37801 gcctgcgcaa ttcttcaagc gcacgtaact cgtcggaaga ctgttcggga ttcaccctgc
    37861 cgtcagacat cgactcttac tcacctgcaa tccgggaata gggagcggcc cagggaaatg
    37921 acgttaaata tttctcgttg agctgggggg ttttgattat agacggtgaa actaaagatt
    37981 gacatccaag gcggggagcc gcgcggccgg gagcgcggac gccttacgtg tcacgggcca
    38041 gagggcttcg agtagtttca ccgggccgcg catttttcgc acttacagcc gacgggcttg
    38101 atgtggtcgt cgaaatactc cttgccatag tcgtagttga tctcctcacc cgccttgatg
    38161 cgcctctttg accatatcca cacgcggcgg ccggtgacga ttgcttcggc gttcggccgg
    38221 cacgagtggt tgatatagcg ggcggtgttg ctgcgcggag tgccgtcgat agaccacttc
    38281 gtattgacgg cgaagaagta tttcccccag tttctcctct cgacctcttc attggagacg
    38341 agcgggccgg tgtactcgat gattcgtttg ccggccggga tgggttccaa cgcgaagaga
    38401 cccagccccg tggaggtgcg tctcacggcg tacttttgat tacccttggt catgttcgag
    38461 atgataccaa cttcttatct acggtataat cattcttatg aataaacgtg ctaaaaaacg
    38521 tgtgaaggaa gccatcgaga agcaaaatct ggaacgctcg caggccagca aaggactgca
    38581 acccacttcc aaaggagtgc cgcagcggcg cggttttacg ccacggccgg ataagaagcg
    38641 aggttgagcc ggcccgcatg acttgccgcc cggtctctaa aggctcgtgc gacagtgttg
    38701 aggctttccc acgcccgcga acccgctgcc ggcgacagcg acgcccattc gcccacccat
    38761 cgagttacat atgactgaag ctttatctta caacaaatat ctgcgcgtgc aggagctgat
    38821 cgctttgcag gattgtctct ccgaccccgc gcatcacgac gagctgctgt tcatcaccgt
    38881 ccaccaggct tacgagctat ggttcaagca gatactccac gaaacggacg cggccgtcgc
    38941 ggcgatggac gacgaccgca cgttcgcggc gacgcgcgca ctggcccgcg tcgtcgagat
    39001 cgagaaactg ctcatcacgc agatacacat tctggagacg atgacgccca tcagcttcct
    39061 cgccttccgc gacgagctga acccggcgag cggcttccag tcgatgcagt tccgcgagat
    39121 tgaattctcc tcgggtctcc gcgacaaagc gattctgcgc gccttccgcg acgacgactt
    39181 cgcctccccg cgtctccagc agcgacacga tgcgcccacc ctcgccgaag ccttctacgc
    39241 gctgctgcga cgccgcggcc tcgacgctcc cgcggatgat ttaagcgccc cggaagacga
    39301 acgccgcaag tcttacggcc ggcgcacgcg cgccgtcctc gaaatcctca cacacttcga
    39361 ggagcgcccc gaggagtttc aactctccga ggcgctgatg gcgcacgacg aatacttctc
    39421 gctctggcgc tcacaccacg tcaagatggt cgagcgcatg gtcggctcca agcccggcac
    39481 cggcggaagc gagggcgtcg gctacctccg caccacgctc gacaagaaat tcttccccga
    39541 gatgtgggag gcccgcacgc acctcgacag caagcacggc ggcgcggccg gctgcccgtt
    39601 cgccggtaag tcgtaaacac acgcagcgac gactatgagt atcgaattcc ccgaacgctt
    39661 caacatggcc gactacttcc tctaccacaa cgtggaggag ggccgcgaag gtaagacgtg
    39721 tctgtactac cgcgacgaga cgtacactta cggcgagacg gtgcgccggt cgaaccgcgc
    39781 gggcaacgct ctgcgcgagg cgggggcgga ggtggaagac cgcgtgctga tcgtgttgcc
    39841 ggactgaccc gagttcgtgt ggacgtggtt cggggcggcg cgcacggggg ccgtcatcac
    39901 gatggtcaac ccgctgctgc cggcggagga ttacaggtat tacctcgaat acacgcgcgc
    39961 gcgcgtggcc gtcattcacg agtcgctgct tgataatttc actgaagccg cgcggggcgc
    40021 acgctacttg aagaacgtgc tggtcgtcgg tgaggagcgt ggggattttc tgtcgttcga
    40081 tgaggcaacg ggcgcgcagg ccgatgagtg tgagccggcc gacacctcgc gcgacgacat
    40141 cgccatctgg ctcttcacct ccggctcgac ggggcacccg aagggcgccg tacacctcca
    40201 gcacgatttg cctttcaaca ccgagtgcta tgcgaagcgc gtgctcggcg tgaatgaatc
    40261 agacctcacc gtctccgtcc ccaagctctt cttcggctac gccacgggca cgaacctgct
    40321 gttccccttc gccgtcggcg gcgcgaccgc gctcttctcc gaacgctcga cgcccgagaa
    40381 gttgttcgag atgatcgaac gcttccggcc gtcgatcctg acgagcgtgc cgacgatgat
    40441 cggcgggatg ctcaacgtcg agcgggccgc cggccgtgac ctctcgtctt tgcggatgtg
    40501 cctgtcggcg ggcgaggcgc tgcccgtcga gttgtataac cgctggatgg agaccttcgg
    40561 cgtggagatt ctcgacggca tcggctcggc tgagatgttt catatctaca tcacgaaccg
    40621 gccgggcgac gtgaagcccg gcagcctcgg ccgcatcgtc gagggctacg aggcgcagat
    40681 cgtcggcggc gacggccggg cggtgaagac cggcgagatg gggacgctgc gcgtgcgcgg
    40741 cgattcggcc gcgctctgct actggcagtc gcacgacaag tcgaaggcga cctttgcggg
    40801 cgactggtgt acgacgggcg accagttcca cgccgacgaa gaggggtact tctggtatca
    40861 cggccgcacc gacgacatgc tcaaggtctc cggcatcttc gtcgcgccgg ccgagatcga
    40921 gaactgcctg ctccagcaca acgccgtgct ggagtgcgcc gtcgtcggcc acgacgacgg
    40981 ctccggcctc gtcaagccgc gcgcctacgt cgttttgcgc gagggacgcc agccgggccg
    41041 cgagatggag gaagacctca agggcttcgt caagtcgaag ctggcgccct acaagtaccc
    41101 gcgctggttc gagttcgtcg agtcgatccc gaagaacgac cgcgggaaga tcgaccggaa
    41161 gcagctaaaa cagtgatgag tgatgagtga tgagtgagta agagagacaa acccattttc
    41221 tctttcttat aactcatcac tcatcactca tcacggccct tatgcctttc tctacaagca
    41281 tcgccgtccg cttcgccgac gtggacgccg ccggcctcgt ctactacccc gtgctcttcc
    41341 actactgcca tgtggcgatg gaggagttct tcgcggcgcg ttgcggcgtc gcctatcacg
    41401 agttggtggc cggggagcac atcggcttcc ccacggtcag cgcgcgggcc gaattcttcg
    41461 cgccgctcgt ctacggcgac gtggcggagg tcgaagtctt cgtctcgaac gtcggccgca
    41521 gctccgcgac cttcgagtac cgtctgcgcc gcgcctccga ccgcgcgctc tgcgcctccg
    41581 cgacgctcgt gcaagtcgcc atgcacctcg acgcgcgccg cgccgtcccc gtccccgaac
    41641 ccctccggcg cgcgttcgcc gagagtgttg attgaatgtc cgtggacacg gccgccgcgc
    41701 aaagaggaag cgtccgcggc ggggaacctc gtcccttcag cttttttaca tcgccgcgga
    41761 tttggcctat aatccgtcgt gctcgccggg ctttgaggcc gcgccccgcc ccttcttaag
    41821 ctgccagact atacggcccg agtctctgga ggtcagtcac atgcatcagc gaatttacct
    41881 ctcactgctg gtcgcgctgt ttctcgcggt cggccccatc gcccgcgtcg gcgagtgcgt
    41941 cgagctgagt ttcgcgggcg cgccggcggt cgtcgtcagc gccgaggcgg ccgacgacac
    42001 gccgtccgcg aagccgaagg gcaacgggtt cgtgcgcgct atcaccgcgc cgttccgcgc
    42061 gctcgggcgc ctgttcagcg gcgggaagaa gagggacgag gcgaagaagc aaaggcccgt
    42121 ggaggagacg ggcgcgcgcg ccgtcgcggc cgtcacagtc gtgaccggcc cgcgcgagga
    42181 ggtcaagccg gagaagcgta aggagccggc cgccgcgcgg cacacgaccc cgacgccggt
    42241 cgccgcccgc ggcgaagtga agaagacggc cgctgcaccc gacagacaga tcgcgccggg
    42301 cggcggtgtc gccgccgagg cgaccgcgga ggttgggccg tcgcagggcg cgcgcgtcgt
    42361 caggccggcc gaggggcagc ccgccccggc gcggctcacg ccgccgaagt ggattcccgt
    42421 cattgaaggc atcggccgtg acccgctcac acaggggcgc gctctgttgc aacacggcta
    42481 cccgaacgag gccgtttccg agctgtccat tgccgccgtc aacggcccca atctcggcga
    42541 ggccaacaac ctcctcggcc tcgcctacga ccgcctcggc ctgcacgctc aggccatcga
    42601 cgcctacgag cgctctctct cggccgcgcc cgacgacgcg caggtgctca acaacctcgg
    42661 ctattcgctc ttcctcgtag gccgtcacgc cgaggcgctc ggtcgtctga agcgtgcgga
    42721 gcgtctcgcg cccggctcgc cctacgtcct ccagaacatc gccttcgtct acgcgcgcct
    42781 cggcaagtac ggcgacgctt tcaagacgct cgcacgcgcc ttcggcgact acgacgcgcg
    42841 tctcaagacg gccgaactgc tcgaacaggc cggacgcaac gacgacgcgc tcaagcacta
    42901 cgatgccgcc ctcaaacttc ggcccgtctc ccccgccctc ctcgaacgca tcatcgccat
    42961 ctacgagcgc acggggcgca cccgcgaggc cgaggccgcc cgccgcaccc tcggccagcc
    43021 gcccaacaag cagaagaccg ccaccggcgg cgg
//
LOCUS       DASVES010000154        68523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_995400, whole genome shotgun sequence.
ACCESSION   DASVES010000154 DASVES010000000
VERSION     DASVES010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 68523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995400"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1419
                     /locus_tag="VF240_06050"
     CDS             67..1419
                     /locus_tag="VF240_06050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7174827.1"
                     /translation="MKLLYITAGAADMYCGSCLRDNALATELMARGHDVALLPMYTPT
                     LTDEPNVSGGRVFFGGVSVYLEQYVPFFRKSPAWLDKLWDSKLLLKLAARRSLKTNPK
                     MLGEMTVSMLRGEDGNQRKELEKLIEWLRHESPPDVISLPYTLVIALARPLKEALGRP
                     VCCTLQGEDLYLEGLQEPYRTESKRLIRENIQHVDAFIAVSEYYARFMPGYLGIPREK
                     IRVVPLGINLQGYGPREEDGGAVATNHDRRPFTVGYFARVAPEKGLHVLAEAYKLLRE
                     SGRLKDARLEAAGYLGADHREYLAGVERKMREWGLAEEFHYRGVLDRVEKIDFLRSLD
                     VLSVPATYDEPKGIFLLEAMACGVPVVQPRRGGFTEIVERTGGGLLVAPDDPASLADG
                     LQKIHDDPAFAADLGRRGLQGVREHYAVERMAERALEVYESLADTNARSVETVQAGAA
                     "
     gene            1537..2205
                     /locus_tag="VF240_06055"
     CDS             1537..2205
                     /locus_tag="VF240_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7174828.1"
                     /translation="MLEVSNLYKEYPTPQGPLAILSDVSLALRRGDAVSIMGPSGSGK
                     STLLYIVGALERPTGGRVTLDGQDPFSLREKELAAFRNERVGFVFQDHCLLPQCNVIE
                     NVLAPTLVSKNGDDAAGRARELLEQVGLSGRTSHRPAELSGGEKQRVSLARALIRRPS
                     LLLCDEPTGNLDARAAESVASLLFELHRRQETILIVVTHSAELAARFPLRYELVGQQL
                     RPRQ"
     gene            2202..5795
                     /locus_tag="VF240_06060"
     CDS             2202..5795
                     /locus_tag="VF240_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7174829.1"
                     /translation="MTTATLLRRNLTFFWRTNLAVVLGVAAATAVLAGALLVGDSVRA
                     SLRNIFLGRLGRADHVVSSAGFFREALAEDLRGDARFASGGFDGACPLVILEGAVTHE
                     ETGRRAAGVAVYGVDERFWEFHGVDGAGEIAPRRRDVLASPSLAGELELKAGDVLLLR
                     VEKPSSIPAESLHGRKDDLGRTIRLTARDTLDAARMGDFSARAQQSAVRAVFVPLRLL
                     QQDLEQDGRVNSLLVSTAGGDDGADEREDAARTATLEAIIKERASLEDLGIRLRALPE
                     RGSISLETESAVVTDALYETAREAARASGLRAAPVLSYLANTIRVGAREVPYSLVTAI
                     DDETFEGLRRTATQTQGDGVTGQQGDGATGVDSSASQTSSAASPSPPTDSQPPTPILL
                     NEWAALDLGARTGDELQLEYYVWKEDNRLAAESARFRVAGVVPIAGLAADRDLVPVYP
                     GITESESLAEWDPPFPVELKRVRRQDEDYWKQHRTTPKAFVPLEAGQRLWQTRFGRLT
                     SVRIDTPGAAATTVDAPGGAQFQASFNAYRDELKKLIDPARVGLGAYGARAQGLAASR
                     GATNFGEYFLYFSFFIVVSALMLTALFFKLGVEQRLREIGVLRAVGFPPARVRNIFLA
                     EGFVLALLGSLLGLAGAVLYGNLMVHGLRTWWVDAVGTTSIALRVSPWSLAAGAAGAV
                     VAALLCVVWTLRRLVPASPRSLLAGKPEGGRSKGEGVKAGGAARRKLLPFTLYPLPLA
                     LLLAAVGVALLVAAWLKAVGQVAGFFGGGTLLLVALLLAQAGWLRGGRRRGASIQGHG
                     FWPVSRLGFRGATYRPARSVLCIALIASAAFIVVAVDAFRRDDRAASTERKSGSGGYA
                     LLGTALLPVIPDPNTAEGRDALGLAADDETRAALAGVEAARFRLRPGDDASCLNLYQP
                     RNPRILAPSADFVASGRFDFQDSLAESAEEKSNPWLLLGRDTSDATVPVIADANSLNY
                     VLHLGLGDVFELAGAGGEPVRLRVVGALADSVFQGEMLMSESNFRRLFPEQEGYRFFL
                     LDVPPERAARVAGILEERLADYGFDAEATAERLASFHRVENTFLSTFQMLGGLGLVLG
                     TLGLAAVLLRNVLERRRELALLRAVGYNRSHFALMALAENALLLFCGLLTGTLCALLA
                     VAPVSLARGGRLPLASVGLLLAAVVAAGLLSSLLATVVALRSPLIPTLRAE"
     gene            6041..7300
                     /locus_tag="VF240_06065"
     CDS             6041..7300
                     /locus_tag="VF240_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174830.1"
                     /translation="MCKSPVRMFRLAALALALSSLSATGALAQGDWTQWGGPSRNFIA
                     KSKGLASAWPEKGPKQLWTRALGEGYAAIVADGPVLYTMYSQGTQEVAVALDAATGKT
                     LWEHKYDAAAAGMNYEFGAGPHSTPLVAGERVFTVGSTGKFHALDKKTGKVVWSKDLW
                     KDLGGARMDRGYSCSPVAYKNLVIVTLGGPGQSLVALDQKDGRVVWKKQDLPVSPSSH
                     LVVNVDGQDQLIAFMGGAVAGLDPATGELLWSQPHATDFGLNITSPVWGDDNLLYVSS
                     AYGGGSRVIKLTRDKATGKTAASEVWFHRRMRVHQTTAVRVGDIVYGSSGDFGPSPLV
                     AVDVKTGQVLWQDRSFAKANMVYADGKFILLDEDGQLGLATASAAGLKVISKVSLLQD
                     RAWTVPTLVGTRLYVRDRKNIMALDLS"
     gene            7694..8977
                     /locus_tag="VF240_06070"
     CDS             7694..8977
                     /locus_tag="VF240_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174831.1"
                     /translation="MSSRLALAFAAALSFAAALAFAAPVFADNWPQWRGPALNGTSVE
                     RNLPVKWGPEENVSWKLPMPDGSGSTPIVWDERVFLNVAEGDALALWCVDRSKGSVLW
                     KRPLGGGNERMRKHNMSSPSPVTDGRSVYVMTGTGLLKAFDFAGKELWSRDIQKDYGQ
                     FGLQWGYASSPLLHDDALFVQVLHGMKTDEPSYVLRVDKKTGKTLWRVERPTAAVRES
                     PDAYTTPALLRHGKTFEIVVTGGDVVTGHDPATGRELWRAGGLNPDNNPFYRIVASPI
                     AFDGIIYAPTRVRPLLAIRAGGRGDVSKSHLLWSTENGPDVPTPVTDGKYFYVVNDRG
                     IVWCLDAKTGKEVYGAQRIKSGTYSASPVLADGKIYATSEDGVTTVVRAGPQFEVLAE
                     NDLKEHTLSSPAVSDGQLFLRTSQNLYCIGKRQKQ"
     gene            complement(9256..9705)
                     /locus_tag="VF240_06075"
     CDS             complement(9256..9705)
                     /locus_tag="VF240_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7174832.1"
                     /translation="MLWVIFALGAALSWGLYGPILHQGQVELKNPMRALLCVGVAYFL
                     VGVLVPVISLWSQGQLGGFNLKGSAAATAGGALGAVGAVCIIYAFRNGGLPTYVMPLV
                     FAGAPVVNVIVSMILHPPKQAPNPLVYVGFLLAALGAGMALYFKPQS"
     gene            complement(9852..13058)
                     /locus_tag="VF240_06080"
     CDS             complement(9852..13058)
                     /locus_tag="VF240_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7174833.1"
                     /translation="MHHTQRFFPAPFLRLPFFFNTTAAALLLLLCLAPAASAQTTSTL
                     TGDVRDAQGSVVAGARVRAKSLETGLERETVSDDEGLFTFPALTVGAYEVSAEKEGFK
                     NYVADRVRLTVGETATLNIPLEAAGVSDSITVTDETALVNTQTPELSYLVGERAIREL
                     PLNGRNYTDLALLQPGVVAFPHRDGGSVVAHGLGTSINGQDPRSNVYLLDGTPQNDFT
                     NGPAGSAAGTSLGIESIREFRIETNAYSAEFGRNFGGQINAVTKSGTNEFHGSLYEFH
                     RNDNLDARNFFDGAEKPEFRRNQFGGSIGGPVRTDKLFFFGAYESLREDLGRTVRTIV
                     PDLDAREGFISTSQAVAPAGQPCPNRQFVGVSAAVRPYLDEFPLPNTGASRVCGNLAD
                     YVFGFNQQVRQHFFQGRIDYNRSEREQFFARYTFDDADQLLPTDFPQFPRTFLSRNQF
                     FTGEYRRTHSANTINTFRFNFGRTRVGQNVESNTTQALTPFVPGQDLVGGIDIGGIPG
                     RFGPQTSANLRLTQNVFGFEHGLVHSRGRHLIKVGGLAERYQDNMVNPTFSLGVFTFN
                     SLRDFLRNTPARFLGISPGGALDRYWRFTLFGLYAQDTFRVTDRLTLNAGLRYEFSTM
                     PLDTGGRDVSLPNLTDRTPTAGQLYQNPTHKNLSPRVGFAWDIFGDGKTSLRGGFGVY
                     FNTNNQQNLIVTVTNPPATPRPIIANPTFPVPSFAVGSQSIRPIQYDLKNPYLQVWNL
                     NLQRELWFDTVVTLGYAGSRGVHLLRSSDVNTPVPVTQADGSLFFPTGQLRPNTAFTT
                     IELKSSDGNSWYNSAVFEARKRFSRGFSAQASYTFSRNIDTTQASTFFSDATNGTTTA
                     FPEFPGLNYNKGLADYHAKHNFVLNFIWELPFARGMRGAAGKILDGWQLAGIGQMRSG
                     NPLTVFVQRNRSRSQWSPSIGPGQGFDRPSLAPGFTHESAVLGRPEQWFNPDAFVLQS
                     AGRLGTLGRGTFIGPNLRIFDLSAVKNTRLPALGENTVLQLRFEAFNLFNRANFAPPN
                     LLAFTGTTDGELPLSTFGRVRSTVTSSRQIQLGLRLVF"
     gene            13495..13899
                     /locus_tag="VF240_06085"
     CDS             13495..13899
                     /locus_tag="VF240_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX7174834.1"
                     /translation="MTFISVTRLRLRSVRYLPPFLWHTLRSLLQAKRAPGNASAKTKR
                     AADGAYWTLTAWDTAAAMRAYRNSGAHKRAMPKLLEWCDEASVGHWEQASDELPDWRV
                     AHRRMIETGRLSKVNHPSERQRAGEMPAPENI"
     gene            13926..17108
                     /locus_tag="VF240_06090"
     CDS             13926..17108
                     /locus_tag="VF240_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7174835.1"
                     /translation="MKIKAPVAAILLLAFAASAFAQTHRATVRGAVTDPNGAVVPGAV
                     VKIINRETRESRETAAGESGEYAVSSLAPGLYELTVETAGFPKFTQKLSLYVNQELRV
                     DVIMATPTLDAAYVDVETQEELKKDSAAISTVIENRQVENLPLDGRNFFELALLVPGA
                     VPAAPGSAGSVRGDFSFSVNGAREDANNFLLDGVYNLDPKLNTFGVRPPVDAIREFEM
                     ATSTYDASFGRNPGAQVNVVTKSGTNDFHGSLYEFHRNAQFDARNYFAPAGEGKPKYI
                     RNQFGGSLGGPLRRDRTFFFADYEGTRAREGVTRVTNVPTAQERAGDFSQSIFGIPTN
                     PFTGQPFEGGRLPDFFIHPVGRRIAALYPLPNRNVAFQNFVSSPTQRDDQNSFDVRLD
                     HAFNDASHFVARYSFGERDLFEPFTGPTLALVPGFGDDVRRRSQNLMLGETHVFTPRF
                     VNEARVAFSRVASSVTQEASVLNTDVGLPFVSTRARDAGLSFITVTGFSPLGDEGNNP
                     QQSVTNVYQVLDTATYARGRHLVKFGADLRFTQQNAFRDVESRGRLQFSPFFPFTGNA
                     LADLLLGFPLLTSVARVDNPQQLRTQSYNFFIDDSWRVTPRLTINAGLRYEYNTPPVD
                     AEDRSNVYDFSTGTLARVGTNGIPRGGYEADKNNFAPRVGLAWTVGEDGETVVRAGYG
                     VYYDQSPLAPGEALYFNSPYFDNNIFFSLPGLPLTLSDPFPAFFPFPLPDSALAIQRD
                     LRTGYMQHWNLNVQRQLGRSRVVELAYVGSKGTKLLTARDINQPLPSVLPPGLPFVPR
                     PNPRFDDISTLESRASSNYHSLQARFQQRLSQGLSMLASYTFAKSLDDASNFFSSAGD
                     ANFPQNSNNPRAERGRSNFDVRHRLSVSYAYDLPFGRGRRFAADSGLLTTLLSGWQTF
                     GIVTLQSGRPFTVAILPELDVSGTGRSILGFGAGDRPQVVGDPRLSTPTPERWFNTSA
                     FSFPAPGTFGNAGRNVLDGPGYQNVNASLVKNTALGERLRLQLRAEVFNLFNHPNFNL
                     PDNFVGSPTFGRITSARDPRHIQFGAKLLF"
     gene            17128..17799
                     /locus_tag="VF240_06095"
     CDS             17128..17799
                     /locus_tag="VF240_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174836.1"
                     /translation="MSNKIVVPRREFLLAGAAAVPLLGVAASRMKQGVVVEPLAANDA
                     SAAAETCRLTEESQYGPFYREGAPWRAKFCGLEEPGERLTIAGRVTAADNCQPLKDAV
                     LDVWHASAAGFYDNNDPARPFDPARYHLRGRVRTDSDGRYQFETVLPGNYGQGRFMRA
                     KHIHLLVTCAGRAPLVTEIYFAGDKHNDTDGLVRRSLITQLSGAAREKDAAPQRGTFD
                     ITLGK"
     gene            17886..18311
                     /locus_tag="VF240_06100"
     CDS             17886..18311
                     /locus_tag="VF240_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7174837.1"
                     /translation="MVNGFFDFFRRRAEYGPVFIRLVVGWMLIWGTQDNVLSWERMLE
                     FAKFLGERGTPFPLFAAHLSVYTQLICGVLILLGAAVRAAAVPLIINFIAAILIAHLG
                     DTFAGAFPALVMLASALFFLFHGAGKLSVDEALERRGPA"
     gene            complement(18503..19153)
                     /locus_tag="VF240_06105"
     CDS             complement(18503..19153)
                     /locus_tag="VF240_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX7174838.1"
                     /translation="MKTARERLSIEELANCVTHGVGLGLSLVGLAALILLAATRGGTL
                     HLVSGGVYGASLVALYTASTLYHAARHPRTKDALRIFDKCCIYLLIAGTYTPFTLVTL
                     RGGWGWTLFALVWALALSGITVTVMFGERFKAATLGSYLLMGWLAVVAVKPIYELVPA
                     GAILLIIAGGLAYTTGVFFFACDRIRHNHAIWHLFVLAGSVCHYLAVILYVMPSNS"
     gene            complement(19310..19702)
                     /locus_tag="VF240_06110"
     CDS             complement(19310..19702)
                     /locus_tag="VF240_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174839.1"
                     /translation="MLRTVAALCLFFSLSVVALPQNRNGAWLGGRWEGTGYQMDTDST
                     WTMVLTARGRKFLIEYPSLDCGGEWRLLSINSRRATFRERITRNREACADRGRVVVEK
                     LSRRQVAFRYSHRGASDVSASAILNRKK"
     gene            complement(19816..20739)
                     /locus_tag="VF240_06115"
     CDS             complement(19816..20739)
                     /locus_tag="VF240_06115"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX7174840.1"
                     /translation="MSESILHNPRRLDRENLDYSVCIQFFLMHAACLLAVWTGVSAVA
                     VAVCVALYVVRMFAITAGFHRLLAHRSYKTGRVFQFLMAFAGTAAYQKGPLWWAAHHR
                     RHHLYADTDDDFHSPVSRSVWRSHVGWFMSRESRTTDAPLVSNLMKFRDLRLLNRFYA
                     LPPIILAGATFLLGAALERYYPSLGTSGWQMLVWGFFISTVLLYHGTFTVNSLAHLFG
                     SRRFETKDDSRNNLFVALITLGEGWHNNHHHYPSSERQGFYWWEIDMAHYTLRMLSWV
                     GVVWDLRTPPAHVTSPLGSPAPNKSLEPTAS"
     gene            complement(20857..21624)
                     /locus_tag="VF240_06120"
     CDS             complement(20857..21624)
                     /locus_tag="VF240_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX7174841.1"
                     /translation="MNSARIEAYLDRINHQYSIAPTAETLRALQLAHLSAVPFENLSI
                     HAGQSIVLDDEALFTKIVRRRRGGFCYEANGLFCVLLRALGFNVSMLSAGVANAEGGF
                     GPDFDHMTLLVDLEERWLVDVGFGDSFREPLLLDARGEQVQGSRAYRIVPDGDHFRLM
                     QRDGDGEWNPQYRFSLRRHEFADFEEMCRYHQTSPQSHFTKGRICTRSSGEGRVTLSE
                     MRLITTSLDGERREQTLSSKEEYDAALREHFGIVMDG"
     gene            complement(21645..22178)
                     /locus_tag="VF240_06125"
     CDS             complement(21645..22178)
                     /locus_tag="VF240_06125"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7174842.1"
                     /translation="MAELEAGLSEIRLSPSDEGVLELIVRRPREDAREILEEGELHTE
                     VGLVGDSWSARGSSRTPDGSPHPDTQLNVMNSRAVALVARDKGRWPLAGDQLYIDLDL
                     SDENLPAGTRLALGSAVIEVTAQPHTGCKKFVARFGLDAMKFVNSTVGKQLHLRGINA
                     RVVQPGVIRVGDVVRKI"
     gene            22340..23284
                     /locus_tag="VF240_06130"
     CDS             22340..23284
                     /locus_tag="VF240_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174843.1"
                     /translation="MNADFESELAAPAGSYRRPASFETLNRRLAEHLLWLARRGDALL
                     QTEPWSENLRAQSRRRGVELVSPAEAGDQSTRVFTPWGWSPSAVAAGEEAGAVLRSIP
                     FAVAARVNSKLWSHALEVELGIAQAGAALAATFEELQEAVARACPEPDDKWVVKSPYG
                     FAARDRVLGRGPSLEEPQAKWSRRRLGRGETLIFQPWLKVVREYGVVLEINPDGSFEV
                     QGISDLQTNGAGTGTGYILGRAPEPRRAAELTRVASLVAARLFAEGYTGPVGIDALEH
                     AGGLHPLLEINARYTMGFVALAVERSLKPAAPVFWSTK"
     gene            complement(23459..23965)
                     /locus_tag="VF240_06135"
     CDS             complement(23459..23965)
                     /locus_tag="VF240_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174844.1"
                     /translation="MLRHFLAALAYRTQKALRDAPAEFGAFRAGDKVRTPAELVRHMT
                     SVLGYARTFFVGGQYRPAPLPSLNEEILRFHEMLEELARHIEAGTPFLEGMTAERLLQ
                     GPFSDAMTHAGQLALLRRLAGHPVPPENFIVADIDAARLGPDQAAPISPDDVWPEATK
                     GGPSLPQT"
     gene            complement(23992..25053)
                     /locus_tag="VF240_06140"
     CDS             complement(23992..25053)
                     /locus_tag="VF240_06140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174845.1"
                     /translation="MSINEEKLNQLLGKFVADFGAALHAGLVVIGESLGLYKALAQAE
                     QPLTSAELAERTNTTERYVREWLCSQAAGGYVEYDAQTQRYFLTEEQAFALADEQSPA
                     YLPGAFLLAVSSLRAVPKITEAFRTGEGFGWHQHDHGLFRGTELFFRPGYAANLVNAW
                     IPAFDGVEAKLKSGAKVADVGCGLGASTILMAQAFPNSTFVGFDYHDKSIEMARQKAA
                     EAGVAERVEFAVAKAKDYPGDNYDFVTFFDCLHDMGDPVGAAAHVKDSLAPDGTWMIV
                     EPFAGDRVEDNLNPVGRIYYSASTLLCTPASLSQEVGLALGAQAGEARMREVVTGGGF
                     KHFRRATETPFNLIFEAKP"
     gene            complement(25390..26913)
                     /locus_tag="VF240_06145"
     CDS             complement(25390..26913)
                     /locus_tag="VF240_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174846.1"
                     /translation="MLSEESFNKPAPWYRRPPVIIALVSVVALAAVAAYFFATRGGAP
                     GSQAHYDELERHRAAQQQQAATDGTQAAPAEQAAAAAPVEGQPAVEGAAAQPGAPAGE
                     QPATAAGAATAPAAGAAGGGRRYWTNFRGPSRDGRYEERQVKTSWPAEGLQLLWKQPV
                     GGGYASFVVADGTAYTIEQRRGQEVVAAYNASTGRELWTNAWNATFSPDDTGDGPRST
                     PTFDDGRLYALGATGEFRCLDAKTGRAIWSKNILSDNGAQNIQWGQAAAPLIVDDKVI
                     VMPGGTSGKTVVAYNKMTGARVWNSMSDQASYTSPVLTTLAGRRQIVVVTARRLAGLA
                     PEDGAVLWEHPWSNGYGINVSQPIVVAANRVFISSGYGKGSALVEVAGSGNTYTARAV
                     WENTSLKAKFNSPVYHDGHVYGFDESIMACIDVNTGERKWKGGRYGYGQVIFASGHLI
                     VTTEEGDVVLVRATPAAHTEVARFKAVEGKTWNVPVIADGRLLVRNANEMAAFKLSD"
     gene            complement(27056..28402)
                     /locus_tag="VF240_06150"
     CDS             complement(27056..28402)
                     /locus_tag="VF240_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174847.1"
                     /translation="MKLAIRTSIALVICLFSLLALSATGAAADPANWPQWRGPASLGI
                     STERNLPEEWTPERNIKWKAAIPGKGHSSPIIWGKRVFLTTAVEGAVVPGAKAVPHLD
                     EGGKESKHPDSVGADRQHAFKVIALDRDTGKILWERTAWEGTPYDDRHRKSSYAASTP
                     VTDGQRVYAFFGAEGLYAYDFDGKLAWKADLGKLATAGMGTGISPVLHENLVIIQADE
                     ENGEKSFIAGLDKKTGKEVWRVPRKVQVTWATPILVKTAKRTELITSGNEAIIAYDPA
                     TGKELWRSKGLESNAIPSPVATSDMVFITAGFPAKIAYAIRLGATGDLTGTPNIAWKH
                     EKGVAYVPSPILYGEHLYMLTDKGIMTALDAKTGKVVYEGGRVPIPSSFTASPVAYDG
                     KILLTSEDGDTFVIKAGPVHSVVRTNSVGEPVYASPAIADGKIFIRGEKNLYAIGK"
     gene            complement(28668..30056)
                     /locus_tag="VF240_06155"
     CDS             complement(28668..30056)
                     /locus_tag="VF240_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174848.1"
                     /translation="MKRFALSLLCLFSALSAGARAQTQNQNWPQFRGVSASGVTDGKA
                     TPAAWDAAKSQNVAWKTPIPGLSHASPVVWGDKVFVITAISGAAKDETRFGLYGDVDP
                     VKDDSKHTYKVYALDKRTGKVLWERAAWEGVPKVKRHPKSTHAAATPATDGKYVVALF
                     GSEGLYAYDLNGKLLWKQDLGTLDAGWFYDPDYQWEYGSSPIIYKNMVIVQADIQKDS
                     FIAAYDIKTGKRLWMTPREEIPSWSTPTVVEGKTRAELVTNGTKAIRGYDPATGKELW
                     RLTPNSEVITPTPIFGHDLIYVTSGYRPIQPIYAIKPGAMGDITLKEGKESSDSIAWS
                     KQRGGPYMPTPIVYGDHLYTISNQGVVTAYNAKTGERLYQERVGGKGGAFTASPVAAD
                     GKLYFSSEDGEVFVVKAGPKYEFLGINPVGEVIMATPAISDGLVIVRALKHVYAFGEA
                     GAAKDKVSKSGN"
     gene            complement(30164..31069)
                     /locus_tag="VF240_06160"
     CDS             complement(30164..31069)
                     /locus_tag="VF240_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7174849.1"
                     /translation="MKLRRLLPLLLLLLLAPPAAIAQTATPTPTPSPAPTATPTPVPA
                     ATPSPEKQKLNDELYEAVRKGDVAAVKATLDKGADVNAKFRYGATALFKAAERGNADI
                     VKLLIERGADVTVRDTFYGATAMTWALDKGHVDAVRAILEKSADDAGDVLSTGARGGN
                     AALVRAALDKGNLKPESMTGAFVAATEAGDKGVEIVEMLKKAGAQPPAEVDAATLQTY
                     VGRFKGTQGPELVFSVKDGKLIAAATGQPPLTMYALDKMTFKPTAFDGITVIFTPEAG
                     KVNGFTLKQGPATIPYTRVEETKQQ"
     gene            31521..33728
                     /locus_tag="VF240_06165"
     CDS             31521..33728
                     /locus_tag="VF240_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174850.1"
                     /translation="MLFSRRFVLLALSTLLLAPAALAQKLTGPVDWAEWRGPLRDGRS
                     FEKNLPSKWSPAGDGLLWKAPYGGRSTPVVFGDRLYLQNTAEKGEREQERVIAFNADT
                     GKLVWEHRFNVYLSDVPAHRVGWASPAVDPATGNVYVLGGGGTLLALSGRDGKVLWER
                     TLGEDFGLLTTHGGRTMSPIVDGDLVIASGVMFVWGQHGQGRHRLVAFDKRTGEAVWV
                     SAPGGRPYDTTYSPPLIANVAGTRLLLQGGSDGQVYAVKPQTGEFVWKYEISKRGLNT
                     GVVVSGTTAYLTHSEENLNSSEMGMLTAVDATAKGDIKPEHVKWTVTGWQGGFSSPIL
                     DGERLYQIDNGANLGAFDTNTGKQLWLQNLGTIQKASPVLADGKLYVGTENGKFFIIE
                     PSATGAKVLDEDQLGTAALPESIIASVAVSRGRIFLVTDAALYAFGKKSNAAPPPLPG
                     DPVSGNSNPTVAHVQVVPTELLLRPGDKASFRARLFDERGNFMREEKATWTLEGLKGA
                     VTETGQFTAAGDAAQAGLVKATVGGVAGAARVRVIPPLPWAENFDALEPNASPAHWTN
                     ATMKFAVREMEGNKVLVKTTEGSSLLSRSRAYMGQPDLHDYTVEADVRGAERRRTMGD
                     AGVIAQRYALTLYGNGQRLSIEPWQPEVERTASVPFEWKKDTWYRMKLRVENLPDGRV
                     RARGKVWLTGQPEPEAWTIEKVDALPNRVGAPGIFGNALAEIYFDNLKVYANN"
     gene            33730..34782
                     /locus_tag="VF240_06170"
     CDS             33730..34782
                     /locus_tag="VF240_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174851.1"
                     /translation="MNEDAQHATQATTPQQQQQGPTPERIFLTLTGYQQAAALKAAIE
                     LDIFSAIAEGDDTSEKLAARLPAAERGARILCDYLTALQFLAKEDGRYQLTPESKLFL
                     DRRSPAYVGGVSGFLSRPYMIEAFQNVAECVRKGGTMLEDQGTVTHENPIWEDFARSM
                     AAMMQPSAQAIARITGAAEMEECKVLDIAAGHGIFGITIAEQNPRAEVYAVDWHNVLQ
                     IARENAASAGVASRYHMMPGSAFEVLFGDDYDLVLFTNFFHHFDPPTCEALMRKAHAA
                     LKPGGRAVTLEFVPNEDRVTPPAEAAFAMTMLASTPSGDAYTFSEFDQMFRNAGFARS
                     ELHPSPPEHIIVSTKQ"
     gene            34816..36354
                     /locus_tag="VF240_06175"
     CDS             34816..36354
                     /locus_tag="VF240_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174852.1"
                     /translation="MKNIRRSRVIILLTAVFAAVAAGGLLLTAHGSLPNPSDPGTGDW
                     PMWGGTPDRNMVTSMKGLPVSWDVKTKKNVKWMAQLGSQTYGNITVAGGIVLVGTNNE
                     GARDPKITGDKGVLMAFKESDGTFLWQAVHDKLVAGRVNDWPYQGIASSPLVEGERVY
                     YVSNRGELMCVDAQGFRDKENDGPVTDEKLTGETNPDIIWRLDMMGDEVGSFPHNLCN
                     SSPVSYGDLVYVSTSNGQDESHVNIPAPKAPAIIAVDKKTGKVVWEDASPGDKILHGQ
                     WSTPTVGKIGDTVQVIMGQGDGWARGYDALKGTKLWEFDLNPKDSVWPKTRNEIIATP
                     VIYEGKIYLANGQDPEHGEGVGHMYCIDGTKRGDITKTGMVWHYDKIRRTISTPAIVD
                     GIIYQPDFSGFLHAIDAKTGQAYWVHDMFAAMWGSPLASVAGGVLYLGDEDGDVVVMQ
                     LGKTKKVLAEINMGSSVYSTPVPANGALYIANRNQLFALAEGAGGAPATPKPATASAT
                     PANP"
     gene            36550..37695
                     /locus_tag="VF240_06180"
     CDS             36550..37695
                     /locus_tag="VF240_06180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7174853.1"
                     /translation="MQSFDFQPRTRVIFGEGAVARLGEAARELNFRRTLLVADHGLVA
                     AGHVGHASEILSVAGVEVVAFHEFESNPDTAMVERGRAFAADAGVDSIVGLGGGSSLD
                     CAKGVNFLLSNGGQMRDYLGYGRAARPLLPMIGVPTTAGTGSEAQSYALISDAETHAK
                     MACGDPGASFRVAILDPALTISQPRHVTATAGLDAVAHAVESFVSTRRTPVSEVFSRE
                     AWRLLESSYERVLAVATDVEARGRMQLGAHFAGAAIENSMLGATHACANPLTARYGTE
                     HGAAIAALLPTVVRWNASAAGERYAELLHAARRGGDNESNAAEALARRLEELIAAGGL
                     ASHVRAVVASREDLAALAEDAAGQWTGRFNPRPFDRAGALEVYERAY"
     gene            37795..38961
                     /locus_tag="VF240_06185"
     CDS             37795..38961
                     /locus_tag="VF240_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174854.1"
                     /translation="MREVESEEGQETLARSISRRGFLAVPVGLYLAGHAARAQEFTGG
                     DSWPQFRGNPALTGFTRSGLSDTLRVLWTYEAGESVESSAAIVGGTVYVGSQKGELVA
                     LDLGSGRERWKYGAGSPIGESSPAVVGGTVYVGDLGGTVHAVSARDGKRVWVFKTGSE
                     VKSSPVVSAGKVLVGSYDQHLYALAARGGNVLWKMKTDGAVHCTASVVGGVAYISGCD
                     ERLRAVRVADGRQVFQLNSGAYTGASPAIMAGNAFYGTFNNDVLGINLVAKRVGWRYT
                     NPERQFPFYSSAAAVDARVVVGGRDKAVHCLDARNGARLWSFNTQARVDSSPALAAGR
                     VFVGSNDGRLYALDFFSGDKAQEFNLGGPVSASPAVAAGRVVVGTQDGRVYCLG"
     gene            complement(39222..39734)
                     /locus_tag="VF240_06190"
     CDS             complement(39222..39734)
                     /locus_tag="VF240_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174855.1"
                     /translation="MRLRTPLVTLACLLAAVVAAKAFGPRPNFSGTWELDQKQSHSIP
                     PDMKQTMTVVHNGDKVSLEVKIVNAQGERVQKDEFVLDGKEIDFTPPPPRPDAPPAKG
                     KRKGNWLPNGKGFFLEEEIQTKNPQGQDVTLIIARKWTAWPDGTISIETIQETPRGTF
                     NNKRVFVKKA"
     gene            complement(39887..40441)
                     /locus_tag="VF240_06195"
     CDS             complement(39887..40441)
                     /locus_tag="VF240_06195"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7174856.1"
                     /translation="MKLLTTRILTLLAIATVTLTLSASAIATAATDPPAVGAAAPDFQ
                     LTTNENKQAALKDFRGQWVVLYFYPKDFTSGCTMQARNFQRDLQKYEQAKAVILGVSV
                     DTADSHKEFCAKEGLNFKLLADIDAKVSNAYGSVMEYNGANYSARNTFIIDPEGKIAR
                     VFLKVKPSVHSEEVLAALAELQKK"
     gene            complement(40470..42104)
                     /locus_tag="VF240_06200"
     CDS             complement(40470..42104)
                     /locus_tag="VF240_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX7174857.1"
                     /translation="MIPAIVVFIYLGVVVYIGVFAFRRSRGHHGAEDYFLANRSLGTF
                     VFLMSLFGTNMTAFAILGSSGHAFSNGIVTYGLMASSSGLVIPLTIFLIGTRIWALGK
                     KYGFMTPVQMMRDRWECGHIGTVIFAVQAVLLVPYIIIGIMGGGTTLSAISGGLVPFW
                     FGGAIVAMVVMSYVFFGGMRGTAWVNTFQTILFLVFGAIAVSVIGSQMGGFRAAVEAM
                     EQAPALKPLLTRERISPYYFFSYTFIPLSAIAFPHIAIFCLTAKKMVQFKKTVILYPL
                     CILAIWLPCVFLGVMANRVSDVPAIRAKQEARRVLATQGKTMEPEARDELREQAAGDD
                     VILLLLERFAPLWLAGLLGAGIMAAVMASDSQILALSTMFTEDVFAFYGGKRRFGEAV
                     QVQTGRIFVILLTIFAYAVALRAPESIFELAIQYAFSGFASLSPLLVAALFWRGSTKW
                     GALAVAVWTISAVIAVAIFQSVVPAPAPGGNTVAYAIGATEVLARTPGGTTVYGFMPV
                     VPMVFGSALLMFFVSLVTRKPAAATIAKYFPEKQTA"
     gene            complement(42174..42404)
                     /locus_tag="VF240_06205"
     CDS             complement(42174..42404)
                     /locus_tag="VF240_06205"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX7174858.1"
                     /translation="MRKLLLVIAVAVLYVLHQDFWNWREARPFVFGFMPIGLFYQACF
                     SVACAGLMWLLVTFAWPGHLEEEVARKVISDK"
     gene            42603..43853
                     /locus_tag="VF240_06210"
     CDS             42603..43853
                     /locus_tag="VF240_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174859.1"
                     /translation="MRLIHRIFLLLAAFVMSAVSASAQKTTPPSQTAPPASSSAAATT
                     PSDDDDEVERVSVHVVSVPVSVTDRDGRFVPDLKESDFRVSENGRDQSISYFASVEQP
                     FSVMLLLDTSGSTSIRLRDIQTAAVEFINQLRPQDRVLPIAFDNDAVALLPGWASDRA
                     VLTQAIRSARTGMIMEDKDREVKVGSDGKKVVFQHINTRLYDAFYKSAETLRHIKGRK
                     ALILFTDGYDNSSRLATRASTLTLAEELDALVYVVQFTPYGNYAPKEQPVAGNLGGPV
                     ARPSTAPSVSEIRQSTTQMTAAGVSPASPTRASPIGGGSASVLGARTTRPSEQRHPKA
                     LEVERYLEGLAEKTGGRFYVADDMNKIKLAFAAVADELRRMYSIGYHPTPLAKPGERR
                     EVKINVRRERVAVRARRAYVFRPR"
     gene            43899..46169
                     /locus_tag="VF240_06215"
     CDS             43899..46169
                     /locus_tag="VF240_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174860.1"
                     /translation="MMKTKASLAPTLLCLSLLAPAFAQQPVANPASAPRQQPTQPRPQ
                     QDDDDIVRITSNLVQLDVIVTDKDGRPVTDLRPEDFQIFEDGKEREITNFSFVSTEGP
                     SPGQPTEAGAPQPAPRRTEKALPPMPSAPLHRREVRRTIALVVDNLRMSHLSATSARE
                     GLRKFVNEQMRPGDLVGIYTTRGGSGSLQQFTSDKTQLHRAIDRVRWYPPPLGGVGVD
                     VFEPARDDSTFKPTTPGSGGTRLDGLAVGKEEFGDAETRQRRERMDERNRDLVAYGAL
                     GTLRYIIRGMQEAPGRKSVVLFSDGIPLFSRNDGAASRSFDAMDRVIESATRAAVVIY
                     TVDARGVHIPGMITAADDVRPNATQSLVESRLEKGARAEDGLTYLPAKTGGLFVRGTN
                     NMDRALERVMRDQGGFYLLGYRPTEQTFKGGSKAFREIDVKVRRSGVSVRSRGGFMAI
                     TDEDARRETRGSDKELYALLASPVNAGGVYVRLTARVVGDPRGGGSAARMLLHVDGKD
                     LTFTDAPEGGGYKKLVLDVAAVTLGKEGQIIDEFNRTHTVRTGGEHLDNIVRHGLTYT
                     ADVPVKKPGTYQLRIVVRDQASQRVGSASQLIDVPDLSKQRLTLSGLTLREIVAGGAQ
                     QQSPPDSARPTAETAIAAVTSLSDPAVRRFRPGAILAYGSQVYHTTAGGGPGGTPRLT
                     AQVSLFHNGQPVVKGAPTAVEFDPTRPGWLEGALRLVPQAKPGEYVLQVVVTDESVKD
                     KDRRQTAQWIDFEVIN"
     gene            46187..46927
                     /locus_tag="VF240_06220"
     CDS             46187..46927
                     /locus_tag="VF240_06220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174861.1"
                     /translation="MDDYTSDTYGERHADIYDERLPDYDEAAIEMLVGLAGGGRALEL
                     GVGTGRVALPLAARGVEVNGIDASPRMVERMRAKPGGEAVRVTFGDFAGVEAEGEFAL
                     VYVVFNTIFALLTQDEQVRCFRNVARRLAPGGVFLIEAFVPDPTFHPGGQSVKVGQMT
                     NDLVELHVTQLDAATQMLVGQQIRFDGAGARLYPVQLRYAWPSELDLMARLAGLSLRQ
                     RHAGWRREPFDARSTKHVSVYAREGDAG"
     gene            46957..47970
                     /locus_tag="VF240_06225"
     CDS             46957..47970
                     /locus_tag="VF240_06225"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX7174862.1"
                     /translation="MNSKWHRLSDDELLDMRLCDLPVSIRKTPLEGRIVRLYSELAER
                     GLRFRPHIWLSEDWFSPDGVPGFAIPFYLAHPRLVKLERAQMLEVEGGAEKECLAIMR
                     HEAGHAVSNAFNLHRRRRWREAFGRFTAPYPEHYMPDPTSRGFVLHLGAWYAQAHPAE
                     DFAETFAVWLAPGSRWRKRYVSWPAMKKLRYVDELMREIAGARPPNNSRQKVEPLAEI
                     KKTLREHYRRKRGHYSFKWPAYYDRDLKRIFSDDEAHRASATAASLLRRHRRELGRLV
                     ADVTGVHNYAVDQVLKNMIDRCKELRLRVPLHEENAKERALIMLTAHTMNIVHAGYHR
                     VPL"
     gene            47967..49010
                     /locus_tag="VF240_06230"
     CDS             47967..49010
                     /locus_tag="VF240_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174863.1"
                     /translation="MTKKKKPTVKKLRILVLCQHDLVPPDALPEGVAKETLRWRTEYD
                     VVSTLRSMGHDVYPVGLYDETDVIRTAMQDHEPHVAFNLIEEFDRQPMFDQHVVSYLE
                     LKRLPYTGCNPRGLTIAKDKALTKKILAYHRINVPGFGVFPQGRKVRRPARLRFPLLV
                     KSVTVEGSVGISQASIVNTDEKLAERVDFIHRQTNSPAIAEEFIEGREIYVGVIGNRL
                     LKAYTPWELEMKTLPEGTVNIATGKVKWNPKYQEKVGLVTHAAEVTPELRRRLEHLSK
                     RIYRLLFLSGYGRLDYRITEDGRIYLLEANPNPQIAHNEDFADSAEDCGVKYGPLLQK
                     ILTLGMSYEPGVV"
     gene            49224..50354
                     /locus_tag="VF240_06235"
     CDS             49224..50354
                     /locus_tag="VF240_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7174864.1"
                     /translation="MDLPTETEKKPVWQRRWFLWALIFGVWTFLAFFFTGQVYFSRRI
                     MERPIPWSEVAREQFIYPYMWALATPLILWLAGRYPVERPRLKQHLLIHLLFSTALTV
                     LIMSAFQFVYYVLYLMPQGKTFSPVSMLRATVFNASENYGLYGLIVLLHHVYRYYNRF
                     RQGELRASQLEARLSQAQLQALKMQLHPHFLFNTLHSISSLVHKDPEAADKMLARLGD
                     FLRMTLENSGAQEVKLKQELEFLRCYLEIERIRFRDRLTTEMNIDPKSLDTRVPNLIL
                     QPIVENAIRHGVAPRSAPGRIEIESKQHNGTLRIEVRDNGPGLLPNQRADGLLHKGLG
                     LANTKARLEQLYGAAHRLELAENPGGGLLVALEIPTRREPAL"
     gene            50531..51343
                     /locus_tag="VF240_06240"
     CDS             50531..51343
                     /locus_tag="VF240_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7174865.1"
                     /translation="MASDAPSRIRVLVVDDEPLAREKIREMVKRDAEIEIVGECSSGA
                     EAVSAVRELRPDLLLLDVQMPELGGFEVLQALDGEHPPAVIFVTAYDQYAVRAFEVHA
                     LDYLLKPFDRERFEAAMGHAKAHLRGQGRGGGDSNGAGGPFDRRILDLLEELKAEKRY
                     AERLVVKDAGRVFFLETAEIEWVEAEGNYINVHTQKKSHLLRETISGLEAQLDPRKFV
                     RIHRSAIVNIGSVRELQPWTHGEYHVILRNGTSLTLSRNYRENLRNVLGNGL"
     gene            51685..52437
                     /locus_tag="VF240_06245"
     CDS             51685..52437
                     /locus_tag="VF240_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174866.1"
                     /translation="MRFPLAPNAFALMLCAPIWVAAFETPPCVPPDACATFAESMAIG
                     ATAGGRDDLAGPPMPTEAAPVKIMLEDAQRAVLLEEAYKDARDVLARDDECSDFFGGR
                     DGAQHVLGRLVGQLRDDPALEPNIGIVMKGDYTNIINLQSGLSYRLFTEAIVNSRGPF
                     FRKNDGRRVFPDCGSFAPNTRGVRAAMLLHELGHLIQGANGRWLLPNDGNNPMLAERN
                     SNRIEKKCGRQIKALDAKPAPSDLGTAKRARS"
     gene            52517..52906
                     /locus_tag="VF240_06250"
     CDS             52517..52906
                     /locus_tag="VF240_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7174867.1"
                     /translation="MINVALLDHEVIRAREGVRVEGSAGEIDLARKVESLREVALSLL
                     KQVEQLEESLSARGTARSTDLHAEVQRFETEIIRDALKKTGGHQRRAARMLGVKVSTL
                     NAKIRRYGIRPEDLNDAGGLRLVGEGA"
     gene            53288..53527
                     /locus_tag="VF240_06255"
     CDS             53288..53527
                     /locus_tag="VF240_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174868.1"
                     /translation="MKDGNWQTEVEVIDGEVLRLLERRSQIVALAACAGDYARAARQV
                     DTSAGGEARPRRSRAASLYASQPHPEAHVAAGDLP"
     gene            53715..54686
                     /locus_tag="VF240_06260"
     CDS             53715..54686
                     /locus_tag="VF240_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX7174869.1"
                     /translation="MRLSRFSVFVWGVVAFNLGVIVWGAYVRASLSGDGCGSSWPLCN
                     GEVVPLGAGAKTLVEFTHRATSGVALLLVVALVAWALRALPKRHPARAGAVMSLVFIV
                     VEALIGALLVRYQFVAGNPSASRAVWMAVHLVNTFLLLASLALTAWWASGGTAVRLRR
                     QGTLGLVVYSALAGTLVLAVSGAVAALGDTLFPAGSLAEGLRQDLSPAAHALVRLRLL
                     HPLLALVVGGLVLFAASYANRVRAGLWVGRLSGAAAALVLLQLLVGAFNVALLAPVGL
                     QLTHLLLADLMWIALVLLGAAALSHEAHAAAAPGFEPSGVPSAAGSS"
     gene            54780..55700
                     /gene="uvsE"
                     /locus_tag="VF240_06265"
     CDS             54780..55700
                     /gene="uvsE"
                     /locus_tag="VF240_06265"
                     /inference="COORDINATES: protein motif:HMM:NF002640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX7174870.1"
                     /translation="MGIQDDARTTPNLGLVCITTTDAVRYRTTTRKRLLQFETAEQKR
                     RLRELYAANLSRLDGALDFCLSQDLRLYRMTSGLFPFADDEAGADVLEEFRSEAARVG
                     RRATQRGIRLVLHPDQFVVLSSDSPHVVANSIKILETHALVMDLLEQPRSAWALVEIH
                     GGKSGRAAQLVSVVRDLPDAVRSRLAFENDEYSYSAAEILEVCHAAGVPMVFDAHHHV
                     VSERLESYDHPSVGEMLRSARATWAVPEWQLVHISNGRESFNDRHHSDLIHDMPEAYR
                     DAPWIEVEAKHKELAIEKLRAGWLAQGAAA"
     gene            55729..56361
                     /locus_tag="VF240_06270"
     CDS             55729..56361
                     /locus_tag="VF240_06270"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX7174871.1"
                     /translation="MTMLRESAKKGRKLSRSFYARDDTLLVARELLGKRLVVPAADGA
                     RVSGLIVETEAYMGPEDKASHAFGGRRTARTEMMYARGGAVYVYLIYGLHHQFNVVTG
                     TEGIPHAILVRALEPVEGVDVMRARRGLEDVRRLASGPGKLCQALALDRGYNGEDLTR
                     TRVWLEDAGRPVSPREIARGPRVGIDYAQEYIAKPWRFWVKDNPFVSKKA"
     gene            56632..57570
                     /gene="rnz"
                     /locus_tag="VF240_06275"
     CDS             56632..57570
                     /gene="rnz"
                     /locus_tag="VF240_06275"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX7174872.1"
                     /translation="MQITFLGTSAGVPTRSRNVSCVALRLEQRGEVWLFDCGEGTQHQ
                     FLRCNELKISQITRVFITHMHGDHVFGLMGLLATCGMAGHARRIDVYGPPGLAEYVRE
                     STRRSQTRFSYPAEIHTIEGAGRIYEDDEYSVVCDALVHRAPTFGYRVTEKDRPGRFD
                     VEHARALGIPEGPLYGRLKRGESVELPDGRIVNGRDLTGPTEPGRSVAYCTDTVYCQA
                     SVELARDADVLIHEATFARDEIELARQSMHSTTETAARVALEAQARRLVITHISPRYA
                     EVNPVTPQDLLEEARAVFPDTYLARDFLTLEVPRRV"
     gene            complement(57592..60348)
                     /locus_tag="VF240_06280"
     CDS             complement(57592..60348)
                     /locus_tag="VF240_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7174873.1"
                     /translation="MKSFVRRARGLWRADEIGREIDEEMRFHIDMRAEENVRRGMSPE
                     EARREALRRFGNRTRVRERGYEVRGGGWLETVWHDLRYGARVLRKNPGFAAAAVFTLA
                     LGIGVNTTVFTLCNAFAWRPLPVRDPGALVRVERVNPDGTRAETTSYPDHAYFDEHNQ
                     VFAGMLVYAGPAPLTMSESPSEAGAADAGARAEVAQIAFVSGNYFSLLGGATQLGRTF
                     TSDEDTTQGTHPVAVLSHKYWQRRFGADTRVLGKTFAINGRQLNVVGVAAADFYGVTP
                     LVPDVWVPVAMQNSVLPIFDSRDRGATFFRSVGRLKPGVTTEQAQAEIDVLAGQLARS
                     FPETNERVGAAVEPASSFARLDGFLRTMFFLVLAADLLVLLVACANVANLLLARASAR
                     RREIAVRLALGATRGRLVRQLVTESLMLSFAGGALGLLLAVWTLDVVLKVVLAGLPPE
                     MGVVNTAFDLSPDVRVFAYTLLLTLVAGVTFGLAPALQASRPDLVSALKQADGGAARV
                     RRRGIFRLGLRDVLVGVQVAVCLVLLVGAGLLIRGVRKGQSLDAGYETARFLVVEPNL
                     LSLGHDAARTAEMHRQLSERLQALPGVESVTLSTRTAGARKTAVAAVAEPGAQGGGRQ
                     LRAYYTMVAPNYFETTGIAVLRGRGFTEQETRETAPRVAILSEATARRLFVPGEEPLG
                     RRVRLGVAPARDAAGRQFEPEPYSPSCEVIGVAKDVRSVRISEVDDALVYLPLRPTNE
                     VQVWLLARTRGEPRSVLGAVRDEVERFDRGIYPNVTLLEETVAQQLLPARVLTLACSV
                     LGAMGLVLASIGLYGVISYSVAQRTHEIGVRMALGARRRDVLRLVIGGGMRVVLAGVV
                     AGLIGAAALSKVLVGFLYGLSPLDPLAFGGVSLLLATVALFAMYLPARRAARVDPVIA
                     LRHE"
     gene            complement(60372..60716)
                     /locus_tag="VF240_06285"
     CDS             complement(60372..60716)
                     /locus_tag="VF240_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7174874.1"
                     /translation="MTKRREDKQADLLQGTLDLLILKALSHGAMHGYGVGQRIAQMAE
                     EMLRVEEGSLYPALYRLEEREWITSEWGVSENNRRARFYKLTAAGRRQLHVEEESWRR
                     FALAVGKVLQTA"
     gene            60989..61453
                     /locus_tag="VF240_06290"
     CDS             60989..61453
                     /locus_tag="VF240_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7174875.1"
                     /translation="MSFNKVIVVGNLGKDPEMRFTPQGTPVCTFTVATNERRKDKAGE
                     QQDVTTWFRVTVWGKQAETVSKYLTKGRSVYVEGRMHVEEWTDKDGKLRFTLELNASD
                     VRFIDGAQGAPSEGIPVRQTGQVRESAARAGVGGGGRAPSSAADIDDDSIPF"
     gene            61523..63007
                     /locus_tag="VF240_06295"
     CDS             61523..63007
                     /locus_tag="VF240_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7174876.1"
                     /translation="MRRGVETRAHAFVSPARLLYLRRVKDSTTLVLSFEAGTLLLEGV
                     GPTEKAPTAFRWDERVGRWRAPASSYRQVVSELTRGRVAFHDRARGYHQFDFPTKFLV
                     EPRPYQHEAIREWKAALGCGVVILPTGAGKSLVAQMAIEQVKRSTLVVVPTLDLMNQW
                     YDLLLSCFEAEVGLIGGGFYEVGALTVTTYASAFRFMERLGNAFGLVIFDECHHLPSG
                     SHRYAADLCIAPYRLGLTATPERADGGDQLLEQLIGPTVYRREAREFAGEYLSDYTVE
                     HVSVRLTTEERARYESEREIFRRFLSEKGIGLGSLRGWQMFVAASARTSEGRRAMMAY
                     RESKRIALGTDAKLRVLGELLLRHRREKVLVFTAENEMVYRISRRFLIPAITHETNVK
                     ERRAWLEAFNHGDVLALVTSKVLNEGVNIPDASVAVVLSGSGSSREHVQRLGRILRKL
                     PGKEATLYEVVTEDTTEEQISRRRGGEQFDAKRQRAPTTAEIPF"
     gene            63019..64227
                     /locus_tag="VF240_06300"
     CDS             63019..64227
                     /locus_tag="VF240_06300"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEX7174877.1"
                     /translation="MLTADLAMSWQRGEQVRPRQLATKDPRHLQTAADLVAIFEAHAG
                     RERHELDEALGEYVGTGTDYRVLRGLIKLLTDRCRFETDVSVEPSEIRRALFLKARER
                     HPVAGADGAARLDVAAEVASSLGCDAETVLRSMYADLPAEQRLLEFDPTGPGELIDLY
                     NLAQAQALLYRSVEMRLWVEPQGPDGYRELFGAIKAYRLIHTVRGDAATGYEITLSGP
                     VSMFHRSQKYGVQMAVFLPALLLCRGWRMRAEINTRHGHAYFELDSRQTSLRSHYLEV
                     TPYQNPAAEKFAESWERAGCASLLEPNREVIGHGDTAFIPDFSITHPSGRQFYLEFLG
                     FWTPEHLRARLAEFERHRLRNYLLAVWEDLRGTREPLTNVPPNVVVFKKNLDPSIVEL
                     AISKLVAAEA"
     gene            64492..>68523
                     /locus_tag="VF240_06305"
     CDS             64492..>68523
                     /locus_tag="VF240_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HEX7174878.1"
                     /translation="MIDGPSTRQQSEAAPEQASVTAPESLRGPCITKLVSDKPVEQVG
                     SDDEGAHKRLASALADLMRGEEKGGVSVGLEGDWGSGKTSVLMLFKQELRDAGELIEP
                     SPEDEGQEGEKAAGAALPAPGSGAKDFAVITFDAWAHEKDPLRRTFLETIIPHLLSIG
                     WVRKSDSWRWKRRLQIIAQRLESKTTDSSPRLEPAGVAAVLSLFLVPIGHDLFSEALK
                     TTQFVWDPDRPIAWRLLVGLFLMVLPLLVVASAYVRWWLSGSGERPSLWALLFNKGIT
                     SERTRTFKTANPTSIEFEHEFTQLMKETLEGKVVLDEDEEKEQEEAKRKRDNTKGAVV
                     EADVDYKRRRLVLVFDNLDRVDTADALTIWSTLQTFLGSPNAAQSAWFDRLWILVLYD
                     PRGLRLLWDKIEEKRLGQPGERAAPADPTSAAGVGGQAAKPAPGGSAEASKAGPGGAP
                     GGQDAKPATPAAAVAGAPAGQAPGAVEGWAAPVASAITRAASTSFMDKSFQIRFEVPP
                     PVLSDWRQRLRGFLDDAIPRHAGEFDSILGVYRLYLSETEQYPTYRELKLFANQIGSL
                     HRQWAKYCGPAPTGQEKNWEERFPLSNVAYYVLLRRRGVDVVKGLLGGRLPEDSYKGL
                     LGPVEQARDTLAALAFNVRRRTARQIVLGKPIGDALERGAEGAAKLAELKESHDGFWG
                     ALKDVLDARGGESVATPEDADRLNRAIQCIHRSGMFDSPEPDNDDLGGAVKVLCMRAA
                     AVAEWPSFDSPTAEGLAALCRWARHPKVWHDGWHDRVEFESNLLKNVSRSLQPDEKVR
                     PVEKAGQWLENVSVVAAEIFTEVDDAPEKQQQHAAILDQIAAQLLPEDVEVFTDEGRM
                     LKAKYQEHPPARLSLLLEALMILRNLNPGGSEMQQVINQLVNIDYSLLHYLEDAYSAP
                     EGRERTQALARSMFLHLNEDPSASQRWGISTELEGSARLRELLSASEEDEASASPNAP
                     GVNLAEEFVYVLNDHGRLPLLFELLDKAAERKVVEPFVARCLRVVADDRNFGKILLTR
                     ETLVERWPDIYRLVNGDERPLAGALREHIRSLPADEMALAVRIFDDTHPMKKLYHDGP
                     REYVWLYAEVVGSSAESMKALDQSRKRWTNTIYGLDAERWRREVLEWGALYELTFVLQ
                     QKGQFTVVPKHYVEGMLEAAKAVIEGEPTATRPPERAPTVLLGTSADASVSDALYQSA
                     ADAGWVIPEEFFDQYGRSLAESDQLREGSLARILEPMLKKRNAPGLRWLHGVLAENSE
                     LQERIDFPEFISLAQKDIEEHRNDLAAAIMFEIGKIVGVVDPSAVDHPRFEIVLFDVG
                     PQPASVLDLITLNTSVGSRAKEII"
BASE COUNT        11926 a        22723 c        22428 g        11446 t
ORIGIN      
        1 gacgccggac gttgaataga ctttctttta ctgctcactg ctcaccgctc actgcttact
       61 tgagttatga agcttctcta catcactgcc ggggccgccg acatgtactg cggcagttgt
      121 ctgcgcgaca acgcgctggc gaccgagttg atggcgcgcg gacacgacgt ggcgctgctg
      181 ccgatgtaca cgccgacgct gaccgacgag ccgaacgtga gcggcgggcg cgtcttcttc
      241 ggcggcgtca gcgtctacct cgaacagtac gtgcccttct tccgcaagtc gccggcgtgg
      301 ctcgataagc tttgggactc gaagctgctt ttgaagctcg ccgcgcgccg ctcgctcaag
      361 accaatccga agatgctcgg cgagatgacc gtctcgatgc tccgaggcga ggacggcaac
      421 cagcgcaagg agcttgaaaa actcatcgaa tggctccggc acgaatcgcc gccggatgtc
      481 atcagcctcc cgtacacgct cgtcatcgcg ctcgctcgcc cgctcaaaga agcgctcggc
      541 cgccccgtgt gctgcacgct tcagggcgag gacttgtatc tcgaagggtt gcaggagcct
      601 taccgcacgg agtcgaagcg gctgatacgg gagaacatcc aacacgtcga tgccttcatc
      661 gcggtgagcg aatactacgc gcgtttcatg cccggctatc tcggcatccc gcgcgagaag
      721 attcgcgtcg tccccttggg catcaacctg caaggctacg ggccgcgcga agaagacggc
      781 ggcgccgtcg caacgaacca cgacagaagg cccttcacgg tcggctactt cgcgcgcgtc
      841 gcgcccgaga aggggctgca cgttttggcc gaagcctaca agctcctgcg cgagagcggc
      901 cggctcaaag atgcgcggct ggaggcggcc ggctatctcg gcgcggatca ccgggaatat
      961 ctcgccggcg tcgagcgcaa gatgcgcgag tgggggctgg cggaagagtt tcactaccgc
     1021 ggcgtgctcg accgcgtgga gaagatcgac ttcctgcgct cgctcgacgt gctctccgtg
     1081 ccggccacct acgacgagcc gaagggcatc ttcctgctcg aagcgatggc ctgcggcgtc
     1141 cccgtcgtgc aaccgcgccg cggcggcttc accgagatcg tcgagcgcac cggcggcggc
     1201 ctgctcgtcg cacccgacga cccggcgagc ctcgccgacg gcctgcaaaa aattcacgac
     1261 gacccggcgt tcgccgccga cctcggccgc cgggggttgc aaggcgtccg cgagcactac
     1321 gccgtcgagc gcatggcgga gcgcgcgctc gaagtctacg agagccttgc ggacacgaac
     1381 gcgcgaagtg tcgagacggt gcaggccggc gcggcgtaac agtaccgcga gcgggtctac
     1441 ctacgatgag aggcccgcgt cgcgcgtcca cacccgctcg ctaccgctcg cggtactgtt
     1501 tgtgccggca atcaatcatt ataacgaggg ctaggtttgc tcgaagtctc gaatctctac
     1561 aaagaatacc cgacgccgca ggggccgctc gcgatcctct cggacgtgtc gctggcgctc
     1621 cggcgcggcg acgcggtctc gatcatgggg ccttcgggca gcggcaagtc aacgctgctc
     1681 tacatcgtcg gcgcgctgga gcggccaacc ggcgggcgcg tcacgctcga cgggcaagac
     1741 ccgttctccc tgcgcgaaaa agaactggcc gccttccgca acgagcgcgt cggcttcgtc
     1801 tttcaggatc actgtctgct gccgcagtgt aacgttatcg agaacgtgct cgcgccgacg
     1861 ctcgtctcta aaaacggcga cgacgcggcc gggcgcgcgc gcgagctgtt ggaacaggtc
     1921 ggactcagcg ggcgcacgtc gcaccgcccc gcagagcttt cgggcggcga gaagcagcgc
     1981 gtctcgctcg cccgcgcgct catccgccgc ccgtcgctgc ttctgtgcga cgagccgacg
     2041 ggcaacctcg acgcgcgcgc ggccgagtcg gtcgcctcgc tcctcttcga gctgcaccgg
     2101 cggcaggaga ccatcctcat cgtcgtcacc cacagcgcgg agctggccgc gcgcttcccc
     2161 ctgcgctacg aactcgtcgg acagcagctc cgcccccgcc aatgaccacc gccacactcc
     2221 tccgccgcaa cctcaccttc ttctggcgca cgaacctcgc ggtcgtcctg ggcgtggccg
     2281 ccgccacggc cgtcctcgcc ggcgcgctcc tcgtcggcga ctccgtgcgc gcctccctgc
     2341 gtaacatttt cctcggccgt ttgggccgcg ccgaccacgt cgtctcgtcc gccggcttct
     2401 tccgcgaggc gctggccgaa gacttgagag gcgacgcgcg gttcgcgtcg ggcgggttcg
     2461 acggagcctg cccgctcgtc atcctcgaag gcgccgtcac gcacgaggag acgggccgac
     2521 gcgcggccgg cgtcgcggtc tacggcgtgg acgaacgctt ctgggagttt cacggcgtgg
     2581 acggcgccgg tgagattgcc ccgcgacggc gcgacgtgct cgccagcccg agcctcgccg
     2641 gcgagcttga gctgaaggcg ggcgacgtgt tgctgctgag agtggagaag ccctcgtcga
     2701 tcccggccga gtcgctgcac gggcgcaagg acgacctcgg ccgcaccatc cgtctcacgg
     2761 cgcgcgacac gctcgacgcc gcgcgcatgg gcgacttcag cgcgcgagcg caacagtcag
     2821 ccgtccgcgc cgtcttcgtc ccgctcagat tgctgcaaca agacttagag caggacggcc
     2881 gcgtcaactc cctcctcgtc tcgaccgcgg gcggcgacga tggcgcggac gagcgcgaag
     2941 acgctgcgcg caccgcgacg cttgaggcga tcatcaaaga gcgcgcttcg ctcgaagacc
     3001 tcggcatccg tctgcgcgcg ctgcccgagc gcggaagcat ctcgctcgaa accgagagcg
     3061 ccgtcgtcac ggatgcgctt tacgagacgg cccgcgaggc ggcacgcgct tcgggcctgc
     3121 gcgccgcgcc cgtcctctcc tatctcgcca acacgatccg cgtcggcgcg cgcgaagtgc
     3181 cttactcgct cgtgaccgcc atcgatgacg aaaccttcga gggcttgcgg cgaacggcga
     3241 cgcagacaca gggcgacgga gtgacagggc aacagggcga cggggcgact ggcgtcgatt
     3301 cgtctgcgag tcaaacgtca tcggctgcct cgccgtcgcc gcccaccgac tcccaacccc
     3361 caaccccgat ccttttgaac gagtgggccg cgctcgacct cggcgcgcgc acgggcgacg
     3421 aactccagct cgaatactac gtgtggaagg aggacaaccg gctcgcggcc gagagcgcgc
     3481 ggttccgcgt cgcgggcgtc gtccccatcg ccggcctcgc ggcggacaga gacctcgtcc
     3541 ccgtctaccc cggcatcacc gagtcggaga gccttgccga gtgggacccg ccgttccccg
     3601 tcgagctaaa gcgtgtgcgc cggcaggacg aggactactg gaagcagcat cgcacgacgc
     3661 cgaaagcgtt cgtcccgctc gaagccgggc aaagactctg gcagacgcgc ttcggccgcc
     3721 tcacctccgt ccgcatcgac acgccgggcg cagcggcgac aaccgttgac gcgccgggcg
     3781 gcgcacagtt tcaggcgtcg ttcaatgcgt accgcgacga gttgaaaaag ttgatcgacc
     3841 cggcgcgcgt gggactcggg gcctacggcg cgcgcgcgca ggggctggcg gcgtcgcgcg
     3901 gggcgacgaa cttcggcgag tacttcctct acttcagctt tttcatcgtc gtctcggcgc
     3961 tgatgctcac cgcgctcttc ttcaagctcg gcgtcgagca gcgtctgcgc gagatcggcg
     4021 tgctgcgcgc cgtgggcttt ccgcccgcgc gcgtccgcaa catctttctc gccgaagggt
     4081 tcgtgctcgc gctcctgggc agcctgctcg gactcgccgg agccgtcctc tacggaaacc
     4141 tgatggtgca tggcctgcgc acgtggtggg ttgacgccgt cggcacgacg agtatcgcct
     4201 tgcgcgtctc gccgtggtcg ctcgcggcgg gcgcggccgg cgccgtcgtc gccgcgctcc
     4261 tgtgcgtcgt ctggacgttg cgccggctcg tgcccgcgtc gccgcgcagc ctgctcgccg
     4321 gtaagccgga agggggaagg agtaaagggg aaggggtaaa ggccggcggc gcggcgcggc
     4381 gcaaactttt gccctttacc ctttaccctt taccccttgc cctcctgctc gcggccgtcg
     4441 gcgtcgccct gcttgtcgcg gcgtggctga aggcagtggg acaggtggcg gggttcttcg
     4501 gcggtgggac gcttctgctc gtggcgctgc tgctggcgca ggccggctgg ctgagaggcg
     4561 gacgcaggag aggcgcgtcg attcaggggc atggcttctg gccggtgtcg cgtctgggtt
     4621 ttcgcggcgc gacgtatcgg ccggcgcgaa gcgtcttgtg catcgcgctc atcgcgtcgg
     4681 ccgccttcat cgtcgtcgcc gtcgatgcct tccggcgcga cgaccgcgcg gcctcgacgg
     4741 agcgcaagtc gggaagcggc ggctacgcgc tcctcggcac ggcgctgctg ccagtcatcc
     4801 ccgacccgaa caccgccgaa gggcgcgacg cgctgggcct cgcggccgac gatgagacgc
     4861 gcgcggcgct cgccggcgtc gaggcggcgc gcttccgcct gcgccccggc gacgacgcga
     4921 gttgtctgaa tctctaccag ccgcgcaacc cgcgcatcct cgcgccgtcg gccgatttcg
     4981 tcgccagcgg ccgcttcgac tttcaggact cgctggccga gtccgcggaa gagaagtcga
     5041 acccgtggct gctgctgggc cgcgacacgt cggacgctac ggtgcccgtc atcgccgacg
     5101 ccaactcgct caactacgtc ctccacctcg gcctcggcga cgtgttcgag cttgccgggg
     5161 cgggcggcga gcctgtccgt ctgcgcgtcg tcggcgcgct cgccgacagc gtctttcagg
     5221 gcgagatgct gatgtcggag tcaaacttcc gacgcctctt ccccgaacag gagggctaca
     5281 gattcttcct gctcgacgtg ccgcccgagc gcgccgcccg cgtggccggc atactcgaag
     5341 aacgactcgc cgattacggc ttcgacgcgg aggccacggc cgagcgcctc gcgagctttc
     5401 atcgcgtcga gaacacattc ctctcgacct tccagatgct cggcggcctg ggtctcgtgc
     5461 tcggcacttt gggcctcgcg gccgtgctcc tgcgcaacgt cctcgaacgc cggagggaac
     5521 tggcgctgct gcgcgccgtc ggctacaacc gctcacactt cgcgctgatg gcgctggccg
     5581 agaacgcgct gctcctgttc tgcggcctgc taacgggcac gctctgcgcg ctcctcgccg
     5641 tcgcgcccgt ctcgctcgcg cgcggcggac ggctcccgct cgcctcggtc ggcctgctcc
     5701 tcgcggccgt cgtcgccgcc ggcctgctgt cgtcgctcct cgccaccgtc gtcgccctgc
     5761 gctcgccgct catcccgaca ttgcgcgcgg agtagtaatt aacatggatg gacaggatga
     5821 ataggatgag aaatttgaga tttgaaatct tcaatctcaa atctgctttg tcttcatcct
     5881 agccatcctg tccatccatg ttaattctcc tgctgccgta ttttttacag actttttaca
     5941 cccgccgagc cggggcgtaa tatgattgca gcccggccgc taaacgaagg tcacacgcga
     6001 cgcgtgaagg ctttccgcgc cctcgaagga gattcggaag atgtgtaaat cccccgtcag
     6061 aatgtttcgc ttggccgcgc tcgcgctcgc gctgtcatct ttgtccgcca cgggcgcact
     6121 cgcgcagggc gactggacgc agtggggcgg cccgtcgcgc aacttcatcg cgaagtcgaa
     6181 ggggctggcc tccgcgtggc ccgagaaggg gccgaagcag ctctggacgc gagcgctcgg
     6241 cgagggctac gcggccatcg tcgccgacgg ccccgtgctc tacacgatgt acagtcaggg
     6301 cacgcaggag gtggccgtcg cgctcgacgc cgccacgggc aaaacgcttt gggagcacaa
     6361 gtacgacgcc gccgccgcgg ggatgaacta cgaattcggc gccgggccgc actccacccc
     6421 gctcgtcgcg ggcgagcgcg tcttcaccgt cggctcgacg ggcaagttcc acgcgctcga
     6481 caagaagacg ggcaaggtcg tctggtcgaa agacctctgg aaagatttgg gcggcgcgcg
     6541 catggatcgc ggctactcgt gcagtcccgt ggcttacaag aacctcgtca tcgtcaccct
     6601 cggcggcccc ggacagtcgc tcgtcgcgct cgaccagaag gacggccgcg tggtctggaa
     6661 gaaacaagac ctccccgtgt cgccctcctc tcacctcgtc gtcaacgtgg acggtcagga
     6721 ccaactgatc gccttcatgg gcggcgccgt cgccgggctt gaccccgcga cgggcgagct
     6781 gctgtggtcg cagccgcacg cgaccgactt cggcctcaac atcacgtcgc ccgtgtgggg
     6841 cgacgacaac ctgctctacg tctcgtcggc ctacggcggc gggagccgcg tcatcaagct
     6901 cacgcgcgac aaggcgacgg gcaagaccgc ggcgagcgaa gtctggttcc accggcgcat
     6961 gcgcgtccac cagacgacgg ccgtgcgcgt cggcgacatc gtctacggtt cgagcggcga
     7021 cttcggcccc tcgccgctcg tcgccgtgga cgtgaagacg gggcaggtgc tctggcagga
     7081 tcgcagcttc gccaaggcca acatggtcta cgccgacggc aagttcatcc tcctcgacga
     7141 ggacggccaa ctcggcctcg ccaccgcctc ggccgcgggc ctcaaggtga tctcgaaggt
     7201 gtcgctcctg caagaccgcg cctggacggt gccgacgctc gtcggcacac gcctctacgt
     7261 ccgcgaccgc aagaacatca tggctctgga tttgagctga cgccgggccg cgaacgcgca
     7321 gggaagttag tgacgggccg cggagacgtt gaagcaagcg acgacgagcc gcggagcggc
     7381 ggcagaatgt agccccggtc gtgagaccgg ggtggcagca ggatgaaaag attttagccc
     7441 cggaggggcg aaagaacatt aaccttgacg gtggccgatg ttgatgttct ttcgcccctc
     7501 cggggctttc gtctctttcg cacctgttcc cacggctcgc gccgtgggct acattctgcc
     7561 gccgccttcg gcggctggca tcgcgttcct cccacgcggt gcgtaacgct tctgtcgtca
     7621 tcgtcgttgg aaacttgaat cgcgccgccc gatgtggaat aaaatggcga caaccctgaa
     7681 aggagaatca cacgtgagtt cacgtctcgc cctcgccttc gccgccgcct tgtctttcgc
     7741 cgccgccctc gccttcgctg cgccggtctt cgccgacaac tggccgcagt ggcgcggccc
     7801 ggcgctcaac ggcacgagcg tcgagcggaa cctgcctgtc aagtgggggc cggaggagaa
     7861 cgtctcgtgg aaactcccga tgccggacgg cagcggctcg acgccgattg tctgggacga
     7921 gcgtgtcttc ctcaacgtcg ccgagggcga cgcgctcgcg ctatggtgcg ttgatagatc
     7981 gaagggcagc gtcctttgga agcgcccgct cggcggcggc aacgagcgga tgcgtaagca
     8041 caacatgtct tcgccgtcgc ccgtgaccga cggccgcagc gtctacgtga tgaccggcac
     8101 gggcctcctc aaagcattcg acttcgcggg caaggaactc tggtcgcgcg acatccagaa
     8161 ggactacgga cagttcggac tccagtgggg ctacgcctcg tcgccgctcc tccacgacga
     8221 cgcgctcttc gtgcaggtgc tccacggcat gaagaccgac gagccgtctt atgtcttgcg
     8281 cgtcgataag aagacgggta agaccctgtg gcgcgtcgag cgcccgacgg cggccgtgcg
     8341 cgagtcgcct gacgcgtaca cgacgcccgc gcttctgcgc cacggtaaga cgtttgagat
     8401 cgtcgtcacc ggcggcgacg tggtgacggg ccacgacccg gcgacgggca gggaactgtg
     8461 gcgcgccggc ggtcttaacc ccgacaacaa ccctttctac cgcatcgtcg cctcgcccat
     8521 cgccttcgac ggaatcatct acgccccgac gcgcgtccgc cccctgctcg ccatccgcgc
     8581 gggcgggcgc ggcgacgtgt cgaagtcgca cctgctatgg tcaacggaga acgggccgga
     8641 cgtgccgacg cctgtcaccg acggcaaata tttctacgtc gttaacgacc gcggcatcgt
     8701 ctggtgcctc gacgcgaaga ccggcaagga ggtctacggc gcgcagcgca tcaagtcggg
     8761 aacctacagc gcgtcgccgg tgttggccga cggcaaaatc tacgcgacga gcgaggacgg
     8821 cgtgacgacc gtcgtcaggg ccgggccgca gttcgaggtg ctggccgaga acgacctcaa
     8881 ggagcacacg ctcagctcgc cggccgtctc cgacggacag ctcttcctgc gtacctcgca
     8941 gaacctctac tgcatcggca agcggcagaa gcagtgagca gtgagcagta atgagcgccg
     9001 gaggcgcgga agaaagtagc cacgggcgac cgtcccgtgg aatagccgta aataaaaatt
     9061 gaaagccccg gaggggcgaa agaatctgaa gcctgcctga aacgtggctt gaggttcttt
     9121 cgcccctccg gggctttgat cctttaatcg aactcgtacc cacggctcac gccgtgggct
     9181 acattctttc gcgcctccgg cgctcgttga cgcccgtagc ttaccgcgca ccgctcactg
     9241 cttactgctc actcttcaag actgcggctt gaagtagagg gccatgccgg cgccgagggc
     9301 ggcgaggagg aagccgacgt agacgagcgg gttgggcgct tgcttcggcg ggtggaggat
     9361 catcgagacg atgacgttga cgacgggcgc gccggcgaag acgagcggca tcacgtaggt
     9421 cggcagtccg ccgttgcgga aggcgtagat gatgcagacc gcgccgacgg cgccgagggc
     9481 cccgccggcc gtcgccgccg ccgacccctt caggttgaag ccgccgagct gcccctgcga
     9541 ccacaggctg atgacgggca cgagcacccc gaccagaaag tacgccacgc ccacacacag
     9601 cagcgcccgc atcgggttct tcaactccac ctgcccctga tgcagtatcg gcccgtacaa
     9661 accccacgac agcgccgcgc cgagcgcgaa gattacccag agcatagaag acctctcgga
     9721 agaagggagt caggagtcag gagtcaggag tcagaataaa aacctccgac cgcgccggtc
     9781 tgtggtaaca cggcggccgt ccgtcgtcaa tcttctgact cctgtcttct gactcctgac
     9841 tccctttttt atcagaacac cagccgcagc cccaactgaa tctgccgcga cgaggtgacg
     9901 gtcgagcgga cgcgcccgaa ggtggagagc ggaagctcgc cgtcggtcgt gcccgtgaag
     9961 gcgaggaggt tcggcggtgc gaagtttgcc cggttgaaga ggttgaacgc ctcgaagcga
    10021 agctggagga cggtgttctc gccgagcgcc ggcaggcgcg tgttcttgac ggccgagagg
    10081 tcgaagattc tgaggttggg gccgatgaag gttccgcgcc cgagcgtgcc gagccggccg
    10141 gcgctctgga gcacgaaggc gtcggggttg aaccactgtt cgggccgacc gagcacggcc
    10201 gactcgtgcg tgaagccggg cgcgaggctc gggcggtcga acccctggcc ggggccgatg
    10261 gagggcgacc actgcgagcg cgagcggttg cgctggacga agacggtcag cgggttgccc
    10321 gagcgcatct gcccgatgcc ggcgagttgc cagccgtcca gaatcttgcc ggccgcgccg
    10381 cgcatcccgc gcgcgaaggg aagctcccag atgaagttga ggacgaagtt gtgcttggcg
    10441 tggtagtcgg cgaggccctt gttgtagttg aggccgggga actctgggaa ggccgtggtg
    10501 gtgccgttgg tggcgtcgga gaagaaggtc gatgcctgcg tggtgtcgat gttgcgcgag
    10561 aaggtgtagg acgcttgcgc gctgaagccg cggctgaagc gcttgcgcgc ctcgaagacg
    10621 gccgagttgt accacgagtt gccgtccgag gacttcagct cgatggtcgt aaaagccgtg
    10681 ttcggccgga gctgccccgt ggggaagaag agcgagccgt cggcttgcgt gacgggcaca
    10741 ggcgtgttca cgtcggagct acgcaggagg tggacgccgc gcgagccggc gtagccgagc
    10801 gtgacgacgg tgtcgaacca cagctcgcgc tggaggttca ggttccacac ttgcaggtac
    10861 ggattcttca ggtcgtactg gatgggccga atcgactgcg accccacggc gaacgaggga
    10921 acggggaacg tcgggttggc gatgatgggc cggggcgtgg ccggcgggtt cgtcaccgtg
    10981 acgatgaggt tctgctggtt gttcgtgttg aagtagacgc cgaagccgcc gcgcagagag
    11041 gtcttcccgt cgccgaagat gtcccacgcg aagccgacgc gcggggagag gttcttgtgg
    11101 gtcgggttct ggtagagctg cccggcggtg ggcgtgcgat ccgtcaggtt cggcaaagaa
    11161 acgtcgcgcc cgccggtgtc gagcggcatg gtcgagaact cgtagcgcag gccggcgttc
    11221 aaggtcaggc ggtcggtgac gcggaaggtg tcctgcgcgt agaggccgaa gagggtgaag
    11281 cgccagtagc ggtcgagcgc gccgcccgga gagatgccga ggaagcgcgc cggcgtgtta
    11341 cgcaggaagt cgcgcaaaga gttgaaggtg aagaccccga gactaaacgt cgggttgacc
    11401 atgttgtcct ggtaacgctc ggcgaggccg ccgactttaa tcaagtggcg gccgcggctg
    11461 tggacgaggc cgtgctcgaa gccgaagacg ttctgggtga gccgcaggtt ggcggaggtc
    11521 tgcgggccga agcgaccggg gatgccgccg atgtcgatgc cgccgacgag gtcctgaccc
    11581 ggcacgaagg gcgtcaaggc ttgcgtcgtg ttcgactcga cgttctgacc gacgcgcgtg
    11641 cggccgaagt tgaagcggaa ggtgttgatg gtgttggccg agtgcgtgcg tcggtactcg
    11701 cccgtgaaga actggttgcg cgaaaggaag gtgcgcggga attgcgggaa gtcggtcggc
    11761 aagagttggt cggcgtcgtc gaaggtgtag cgggcgaaga actgctcgcg ctcgctgcgg
    11821 ttgtagtcga tgcgcccctg aaagaagtgc tggcgcacct gctggttgaa gccgaagacg
    11881 tagtcggcga ggttgccgca gacgcgcgac gcgcctgtgt tcggcagcgg gaactcgtcg
    11941 aggtacggcc tcacggcggc gctcacgccg acgaactggc ggttcgggca cggctgccct
    12001 gcgggcgcga cggcctgcga ggtggagata aagccttcgc gcgcgtcgag gtcggggacg
    12061 atggtgcgaa cggtgcggcc gaggtcttcg cgcagcgact cgtatgcgcc gaagaagaag
    12121 agtttgtcgg tgcggacggg gccgccgatg gagccgccga actggttgcg tcgaaactca
    12181 ggcttctccg cgccgtcgaa gaagttacgc gcgtcgaggt tgtcgttgcg gtggaactcg
    12241 tagagcgagc cgtgaaactc gttcgtcccc gacttggtga cggcgttgat ctggccgccg
    12301 aagttgcggc cgaactcggc gctataggcg ttggtctcga tgcgaaactc gcggatgctc
    12361 tcgatgccga gcgaggtgcc ggcggcgctg ccggcggggc cgttggtgaa gtcgttctgc
    12421 ggcgtgccgt cgaggagata gacgttcgag cgcgggtctt gtccgttgat gctggtgccg
    12481 aggccgtggg cgacgacgga gccgccgtcg cggtggggga aggcgacgac gccgggctgc
    12541 aggagcgcga ggtcggtgta gttgcgaccg tttaagggca gctcgcggat ggcgcgctcg
    12601 ccgacgaggt aggagagttc gggcgtctgc gtgttgacga gcgccgtctc gtccgtgacg
    12661 gtgatcgagt cggagacgcc ggccgcctcc agagggatgt tgagcgtggc cgtctcgccc
    12721 acggtgaggc gcacgcggtc ggcgacgtag ttcttaaagc cttccttctc ggcgctcacc
    12781 tcgtaggcgc cgacggtgag cgcggggaag gtgaacagcc cctcatcgtc gctcacggtc
    12841 tcgcgctcaa gacccgtctc cagactcttg gcgcgcacgc gcgcgccggc gacgaccgag
    12901 ccttgcgcgt cgcgcacgtc gcccgtcagc gtactcgtcg tctgcgcgct cgccgccggg
    12961 gcgagacaca gcagcaggag cagcgcggcg gcggtcgtgt tgaaaaagaa ggggagacga
    13021 agaaagggcg cgggaaaaaa ccgttgcgtg tggtgcatga ctgccttatg actccaggtt
    13081 gaacggcgga cgaaactgcc tgcccgttga ctgacgaaca ttctaacagg cgggttctga
    13141 catacataaa cggccccgcc ccttttacaa tcttttgaca gttgccggct gacttttaca
    13201 cgtcgtttac aacaatcccg cccgattccg cgataatgcc tgagctattt gaaaagccac
    13261 ggttgtctgg ccgcgacagc cgcgagcgtc gtcacgcaca gtaccgcgag cggaagcctc
    13321 gcgggtgcgg cctgcgctta aggctcgcct tgcgtgcggc agacccgctc gctaccgctc
    13381 gcggtactgt ggttgtgtgg ttgccctcgc gtgggcggcg cgttaaggtt tgaatcttcg
    13441 tagtacgttt cgccgtacat cctgacgggc gaagattcgg agccttcaac acccatgacc
    13501 ttcatctccg tgacgcgttt gcgattgcgc tcggtgcgct atctgccgcc gtttctgtgg
    13561 cacacgctca ggagtctcct tcaggcgaag cgcgcgcccg gaaacgccag tgcgaagacg
    13621 aagcgcgccg ccgacggcgc ctactggacg ctgacggcgt gggacacggc agccgcgatg
    13681 cgcgcctacc gaaattctgg cgcgcacaag cgggcgatgc cgaaactcct cgaatggtgc
    13741 gacgaggctt cagtcggcca ttgggagcag gcgtctgatg agctgcccga ctggcgcgtg
    13801 gcgcaccggc gcatgatcga gacgggcagg ctctcgaagg tcaatcatcc gtccgagaga
    13861 cagagggcgg gcgagatgcc cgcgccggaa aacatttaag ccgagattaa atcgggagat
    13921 cactcgtgaa gataaaagcc ccagtcgccg ccatcctgct gctcgcattc gctgcctccg
    13981 ccttcgcgca gacgcaccgc gccaccgtcc gcggcgccgt caccgaccca aacggcgccg
    14041 tcgtccccgg cgcggtagtg aaaatcatta acagggagac ccgcgagtct cgcgagaccg
    14101 cggctggtga gagcggggag tatgcggttt cctcgctcgc gccgggcttg tacgaactga
    14161 ctgtggaaac cgcgggcttc cccaaattca cgcagaaact ctccctttat gtgaatcagg
    14221 agttgcgtgt cgatgtcatc atggcgaccc cgacgcttga tgcggcctat gtcgatgtcg
    14281 agacgcagga ggagctgaaa aaagattcgg cggccatcag cacggtcatc gaaaatcggc
    14341 aggtcgaaaa tctgccgctc gacggccgca acttcttcga gctggctctg ctcgtccccg
    14401 gcgccgtgcc ggccgcgccc ggctccgccg gctcggtgcg cggcgacttc tccttcagcg
    14461 tcaacggcgc gcgcgaggac gccaacaact tcctcctcga cggcgtctac aacctcgacc
    14521 ccaagctcaa caccttcggc gtgcgcccgc ccgtcgatgc catccgcgag ttcgagatgg
    14581 ccacctcgac ctacgacgcc tcgttcggac gcaaccccgg cgcgcaggtc aacgtcgtca
    14641 ccaagagcgg cacgaatgac tttcacggct cgctctacga gttccaccgc aacgcacagt
    14701 tcgacgcgcg caactacttc gcgcccgccg gcgaaggtaa gcccaagtac attcgcaacc
    14761 agttcggcgg ctctctcggc ggccccctgc gccgcgaccg caccttcttc ttcgccgact
    14821 acgagggcac gcgcgcgcgc gaaggcgtca cgcgcgtcac caacgtgccc acggcgcaag
    14881 agcgcgcggg cgatttctcg cagagcatct tcggcatccc gacgaatccg ttcacgggcc
    14941 agccgttcga gggcgggcgc ctccccgact tcttcatcca ccccgtaggc cgccggatcg
    15001 ccgctttgta tccgctgccg aaccgcaacg tagcgttcca gaacttcgtc tcttcgccca
    15061 ctcagcgcga cgatcagaac agcttcgacg tgcgcctcga ccacgccttc aacgacgcgt
    15121 cgcactttgt cgcccgctac agcttcggcg agcgcgacct cttcgagccg ttcacggggc
    15181 cgaccctcgc gctcgtcccc ggcttcggcg acgacgttcg ccgccgcagc cagaacctga
    15241 tgctcggcga gacgcacgtc ttcaccccgc gcttcgtcaa cgaggcgcgc gtcgccttca
    15301 gccgcgtcgc ctcctcggtc acgcaggagg cgagcgtcct caacacagac gtgggcctgc
    15361 ccttcgtctc gacgcgcgcg cgcgatgcgg gcttgagctt catcaccgtc accggcttct
    15421 cgccgctcgg cgacgagggc aacaacccgc agcagagcgt gacgaacgtc tatcaggtgc
    15481 tcgacaccgc gacttatgcg cgcggccggc acctcgtgaa gttcggcgct gatctgcgct
    15541 tcacacagca gaacgccttc cgcgacgtgg agtcgcgagg ccgcctccag ttctcgccct
    15601 tcttcccgtt caccggaaac gcgctggccg atctcctcct cggcttcccg cttttgacga
    15661 gcgtcgcccg cgtcgataac ccgcagcaac tccgcacgca gagctacaac tttttcattg
    15721 acgatagctg gcgcgtcacc ccgcgcctga ccatcaacgc cggtctgcgt tacgaataca
    15781 acacgccgcc cgtggacgcc gaggatcgct ccaacgtcta cgacttctcg acgggcacgc
    15841 tcgcccgcgt cggcacgaac ggcatcccgc gcggcggcta tgaggccgac aaaaacaatt
    15901 tcgcgccgcg tgtcgggctg gcgtggacgg tcggagaaga cggcgagacg gtcgtgcgcg
    15961 ccggctacgg cgtctattac gatcagtcgc cgctcgcgcc cggcgaggcg ctctacttca
    16021 actcgcccta cttcgacaac aacatcttct tctccctgcc ggggctgccg ctcaccctga
    16081 gcgacccgtt ccccgccttc ttccccttcc cgcttcccga ctcggcgctc gccatccagc
    16141 gcgacctccg caccggctac atgcagcact ggaacctgaa cgtccagcgg caactcggac
    16201 gcagccgcgt cgtcgagctg gcctacgtcg gctcgaaggg cacgaaactt ctgacggcgc
    16261 gcgacatcaa ccagcctctt ccgtcggtgc ttccgccggg cctgcccttc gtcccgcgcc
    16321 ccaacccgcg cttcgacgac atcagcactc tggagtcgcg cgcctcgtcc aactaccaca
    16381 gccttcaggc gcgcttccag cagcgcctct cgcaagggtt gtcgatgctc gcctcctaca
    16441 ccttcgcgaa gtctctggac gacgcttcta actttttctc cagcgccggc gacgcaaact
    16501 tcccgcagaa ctccaacaat ccgcgcgccg aacgcgggcg ctcgaacttc gacgtgcgcc
    16561 accgtctctc cgtctcctac gcctacgacc tgcccttcgg acgcggccgc cgcttcgccg
    16621 ctgactccgg cctgctcacc acgctgctct ccggctggca gaccttcggc atcgtcaccc
    16681 tgcaatcagg acggcccttc acggtcgcca tcctgcccga gctggacgtg agcggcacgg
    16741 gacgctccat cctcggcttc ggcgcgggcg accggcccca agtcgtcggc gacccgcgct
    16801 tgtctacccc gacgcccgag cgttggttca acacctcggc cttctccttc ccggcgcccg
    16861 gcacgttcgg caacgcgggc cgcaacgtcc tcgacgggcc gggctaccag aacgtcaacg
    16921 cctcgctcgt caagaacacc gcgctcggcg aacgtctcag actgcaactg cgcgcggagg
    16981 tcttcaacct cttcaaccac ccgaacttca acctgcccga caacttcgtc ggctcgccca
    17041 cctttgggcg catcacgtcg gcgcgcgacc cgcgccacat ccagttcggg gcgaagctgc
    17101 tgttctgaga gagcgtcaag gagacatatg agcaacaaga tcgtcgttcc gcgcagggag
    17161 tttttgctgg cgggcgcggc ggccgtccca ctcctcggcg tcgcggcctc tcgcatgaag
    17221 cagggcgtcg ttgtcgagcc gctggcggcc aacgacgctt ccgccgcggc cgagacgtgt
    17281 cgtctcacgg aggagagcca gtacggcccc ttctatcgcg agggcgcacc gtggcgcgcg
    17341 aaattctgcg gcttggagga gccgggcgag cggctcacaa tcgcgggccg cgtgacggcc
    17401 gccgacaact gccagccgct caaagacgcc gtcctcgacg tgtggcacgc gagcgccgcc
    17461 ggcttctacg acaacaacga cccggcgcgc cctttcgacc ccgcgcgcta tcatctgcgc
    17521 ggccgcgtcc gcacggactc ggacggccgc taccagttcg agaccgtcct gcccggcaac
    17581 tacggacagg gccgcttcat gcgcgccaag cacatccacc tgctcgtcac ctgcgccggc
    17641 cgcgcgccgc tcgtcaccga aatctacttc gctggcgaca agcacaacga caccgacggc
    17701 ctcgtccgcc gctcactcat cacgcagttg tcgggcgccg cccgcgagaa ggacgccgct
    17761 ccacagcgcg gcacgttcga catcactctg gggaagtgat aagtgatgag tgataagtga
    17821 taagaaagaa ctgcctctct ttcttatcac ttatcactca tcacttatcc ctcccagagg
    17881 tgttcatggt caacggtttc ttcgacttct tcaggcggcg cgccgagtac ggccccgtct
    17941 ttatccgcct cgtcgtgggc tggatgctca tctggggcac gcaggacaac gtcctcagtt
    18001 gggagcggat gctggagttc gcgaagttct taggcgagcg cggcacgccc ttcccgctgt
    18061 tcgcggcgca cctctcggtc tacacgcagc tcatctgcgg ggttctcata ctgctcggcg
    18121 cggccgtgcg cgcggcggcc gtcccgctca tcatcaactt catcgccgcc atcctcatcg
    18181 cgcacctcgg cgacaccttt gcgggcgcct tccccgcgct cgtcatgctg gcgtccgccc
    18241 tcttcttcct cttccacggg gcgggcaagc tctcggtcga tgaagcattg gagcggcgcg
    18301 ggccggcgtg attgatgaac agtaccgcga gcggtagcga gcgggtgtgg acacgcgacg
    18361 cgagccttca gcgcaggccg cacccgctcg ctaccgctcg cggtactgtc cgctacgagc
    18421 ggcgcgacga agcacttcgc cacgcgggaa atcgtccggc agcgagttga aagcatgtgc
    18481 gtggatttca tcggccgcac gcttaggagt tcgagggcat cacgtagaga atcacggcga
    18541 ggtagtggca gacgctgccg gcgaggacga agaggtgcca gatggcgtga ttgtggcgga
    18601 tacggtcgca ggcgaagaag aagacgcccg tggtgtaggc gaggccgccc gcgatgatga
    18661 gcaggattgc cccggccggc accagctcgt agatcggctt gacggcgacg acggccagcc
    18721 agcccatcaa cagataggag ccgagggtcg ccgccttgaa gcgttcgccg aacatcaccg
    18781 tgacggtgat gccggacagg gcgagcgccc acacgagtgc gaagagcgtc cagccccagc
    18841 cgccgcgcag cgtgacgagt gtgaagggcg tgtaggtgcc cgcgatgagc aggtagatgc
    18901 agcacttgtc gaagattcgc agggcgtcct tggtgcgcgg gtgtcgcgcc gcgtgataga
    18961 gcgtcgaggc cgtgtagagg gctaccagcg acgcgccgta gacgccgccg ctgacgaggt
    19021 gtagcgtccc gccgcgcgtg gccgccagaa ggatcagcgc cgccaagccg accagactca
    19081 gccccagccc cacgccgtgc gtgacgcagt tcgccaactc ctcgatgctc agcctttctc
    19141 tcgccgtctt catctcgcaa ctctttcgtc ctgacgcgca aagactcgcg ccgccgccgc
    19201 cttcaacact tcggcccatg ctaacgccgt cgagactcgg aagacaatac cgagatgcgc
    19261 gcgggcgcgt tggaggacgc gctacggcct ccgcggccga ccgatgatcc tattttttcc
    19321 ggttgaggat ggcggaagcc gacacgtccg acgccccgcg atgtgagtat ctgaaggcga
    19381 cttgccggcg gcttaatttt tcgacgacca cgcggcctct gtccgcgcaa gcctcccggt
    19441 tgcgcgtgat gcgctcccga aacgtggccc gccgggagtt gatgctcaga agcctccact
    19501 ccccgccgca gtcgagcgaa gggtattcga tcaaaaattt ccggccccgc gccgtgagca
    19561 ccatcgtcca cgtgctgtcg gtgtccatct gatagcctgt gccctcccac ctgccgccga
    19621 gccacgcacc attcctgttt tgcggcagag cgacgacgct cagagagaag aacaggcaga
    19681 gggccgccac agtcctgagc attattcaat gcctccgaag ggttgtgatt gatggtcgag
    19741 cttaattata gccctgtgcg aaggggggcg ttcgtccgcg cgccgggctg gagagaacac
    19801 ggatgagacg gccgtctaac tcgcggtcgg ctccaaagac ttgttcggcg ccggactgcc
    19861 cagaggggaa gtgacatgcg ccggcggcgt ccgcaagtcc cacacgactc ccacccacga
    19921 aagcatgcgg agcgtgtagt gggccatgtc gatctcccac cagtagaagc cctgtcgctc
    19981 cgaggacggg tagtgatggt gattgttgtg ccagccctcg ccgagcgtga tgagggcgac
    20041 gaagagattg ttccggctgt cgtctttggt ctcgaagcgg cggctgccga agaggtgggc
    20101 cagagagttg accgtgaacg tgccgtggta gagcagcacc gtgctgatga agaatcccca
    20161 gacgagcatc tgccagcccg aagtgcccaa actcggatag tagcgttcga gcgccgcccc
    20221 cagtagaaag gtggccccgg ccagtatgat gggcggcagc gcgtagaaac gattgagcag
    20281 gcgcaggtcg cggaacttca tcaggttgga gacgagcggc gcatcagtcg tccggctctc
    20341 gcgcgacatg aaccagccga cgtgtgagcg ccacacgctg cgggagaccg gcgagtggaa
    20401 atcgtcatcg gtgtcggcgt agagatgatg gcggcggtga tgtgcggccc accacagcgg
    20461 ccccttctgg taggcggccg tccccgcgaa ggccatcagg aattgaaaga cgcggccggt
    20521 cttgtacgag cggtgcgcga ggagccgatg gaacccggcc gtgatcgcaa acatccgcac
    20581 gacgtagagc gccacgcaga cggcgacggc caccgcgctc acgcccgtcc agacggcgag
    20641 caggcaggcc gcgtgcatca ggaaaaactg gatgcagacg gagtagtcaa gattttcccg
    20701 gtcgagacgg cgcggattgt gaagaatgga ttcggacatt aaccaacctt gttccctgta
    20761 agatgtcatc cactataaag tgtattttcc ctattgcaac tcccggccgg cgcagaccgc
    20821 acagttggca ttcacatttt gcggtatcga tgaaagctac ccgtccatga cgatgccaaa
    20881 atgctcgcgc agcgccgcgt catactcttc cttactcgaa agcgtttgct cccgtcgctc
    20941 gccgtcgaga gaggtcgtga tgagacgcat ctcgctcagt gtgacgcgcc cttcgccgga
    21001 cgagcgcgtg cagatgcgcc ccttggtgaa gtgcgactga ggggaagtct ggtgatagcg
    21061 gcacatctct tcaaagtcgg caaattcgtg gcgacgcagg ctgaagcgat actgcgggtt
    21121 ccattcgccg tcgccgtcgc gttgcatcag acggaagtgg tcgccgtcgg ggacgatgcg
    21181 gtaggcccgg ctcccctgta cctgctcgcc gcgcgcgtcg agcagcaacg gctcacggaa
    21241 cgaatcgccg aagcccacgt ccacgagcca acgctcttcc agatcaacca gcagcgtcat
    21301 gtggtcgaag tcggggccga agccgccttc cgcattcgcg accccggccg agagcatcga
    21361 aacgttgaag cccagggcac gcagtagcac gcagaacagg ccgttggctt cgtagcagaa
    21421 gccgccgcgc cgacgtcgga cgattttcgt gaacagggcc tcgtcatcaa gaacgatgga
    21481 ctgcccggcg tgaatactca gattctcaaa aggcaccgcc gacaggtgcg ccaactgcaa
    21541 cgcgcgcagg gtctcggcgg taggcgctat cgagtattga tgattgatgc ggtcgagata
    21601 agcctcgatg cgagcgctgt tcaaggaaag cctgcccttc aatcttagat tttcctgacc
    21661 acgtcgccga cccggatgac gccgggttga acgacacggg cgttaatgcc gcgcaggtga
    21721 agctgcttgc ccacagttga gttgacgaac ttcatcgcgt ccagcccgaa gcgcgccacg
    21781 aattttttgc agccggtgtg tggctgggcg gtgacttcaa tcacggccga gccgagcgcg
    21841 aggcgcgtcc cggcgggcaa gttttcgtcg ctcaggtcga ggtcgatgta gagctggtcg
    21901 ccggccagcg gccagcgccc tttgtcccgc gccacgaggg caaccgcgcg ggagttcatg
    21961 acgttgagct gcgtgtcggg gtgcggcgac ccgtccggtg tgcgagagct gccgcgcgcg
    22021 ctccaactgt cgcccacgag acccacttcc gtgtgcagct cgccctcttc caaaatttcc
    22081 cgcgcgtcct cccgcggccg tcgcacgatc aactccagca cgccttcgtc gctgggcgac
    22141 aggcgaatct cgctcaggcc ggcttcaagt tccgccatcg ttaaatgtct tacctcgtcc
    22201 accgccaaac ctccttacgc cgcgcgtcct cccggcgtcg tcatggtcga ttataaacct
    22261 atcgcaaccc ggttcctgat gtacgattgc cgtcgggatt atacaatctg catcgcccat
    22321 ggtttcaagg ctctggcaca tgaacgccga tttcgagtct gagctggccg cccccgccgg
    22381 ctcataccgc cgtcccgctt cctttgaaac tctcaaccgc cgcctcgccg aacacctgct
    22441 gtggctggcc cgacggggcg acgcgctgtt acaaacggag ccgtggtctg agaaccttcg
    22501 cgcgcagtcg cgccggcgcg gcgtggaact cgtctctccg gccgaggcag gtgaccaaag
    22561 cacgcgcgtc tttacgcctt ggggctggtc gccgagcgcc gtggcggccg gcgaagaggc
    22621 cggggcggtg ctgcgctcca ttcctttcgc cgtcgcggcg cgcgtcaact caaagctctg
    22681 gagtcacgcg ctcgaagtcg aactgggaat agcacaggcg ggggcggcgc tcgccgccac
    22741 gttcgaggaa ctgcaagagg cggtcgcgcg cgcctgcccc gagccggatg acaagtgggt
    22801 cgtcaaaagt ccatacggtt tcgccgcccg tgaccgcgtg ctgggccgag gcccgtcgct
    22861 ggaggagcca caggcgaagt ggtctcgccg tcgtctgggc cggggcgaga cgttgatctt
    22921 tcagccgtgg ctcaaggtcg ttcgcgagta cggcgtcgtg ttggagatta acccggacgg
    22981 ttcattcgag gttcaaggca tcagcgacct ccagaccaac ggcgcgggca caggcacggg
    23041 ctacatcctc ggccgcgcgc ccgaaccacg acgcgcggcc gaactcacgc gcgtcgcgtc
    23101 cctcgttgcc gcacgccttt tcgcagaggg ctataccggg ccggtcggaa tagacgcgct
    23161 tgagcacgcg ggcggtctcc accccctgct ggaaataaat gcacgctaca cgatgggctt
    23221 cgtcgcgctc gccgtcgagc gcagcctcaa gcccgccgcg cccgtcttct ggagtacgaa
    23281 ataacctgag ggagttttca aacagtaccg cgagcggtag cgagcgggtc tcgcgtgtgc
    23341 ggtgcaggcc gctcatcatg gccgcacccg ctcgctaccg ctcgcggtac tgttgccgat
    23401 gacggcgacg cgtcgggcgg cgggcgttgt agcgtcgcca tcagacgcac gacgtttctc
    23461 atgtttgcgg caacgacggc ccgcccttcg tcgcttcggg ccagacatcg tcggggctga
    23521 tcggcgcggc ctgatccggg ccgaggcgcg cggcgtcgat gtcggccacg atgaaattct
    23581 ccgggggcac gggatgaccc gcgagacgcc tcagcagggc gagttgtccg gcgtgggtca
    23641 tcgcatcgga gaacggcccc tgtagtaagc gttccgcggt catcccttcg agaaaaggtg
    23701 tgccggcctc gatgtgccgg gcaagctcct caagcatctc gtgaaagcga aggatttctt
    23761 cattcagcga aggcaggggc gcgggcctgt actgaccgcc gacgaagaag gtgcgggcgt
    23821 agccgagcac gctcgtcatg tgacgaacca actccgctgg agtacgcacc ttgtcgcccg
    23881 cgcggaaggc gccgaactcg gcgggagcgt cccgcagcgc cttctgcgtg cggtaggcga
    23941 gcgcggccag aaagtgccgc agcaatcctc gtttctcatc agatagaact gtcagggctt
    24001 ggcctcgaaa atgagattga atggcgtttc ggtggcgcgg cgaaagtgtt tgaatccccc
    24061 gccggtgaca acctcgcgca ttctggcttc acccgcttgc gccccgagcg ccagccctac
    24121 ttcctgtgac agagacgccg gagtgcagag caatgtcgaa gcggaatagt agatgcgtcc
    24181 gaccgggttc agattatctt ccactcgatc cccggcaaac ggctcaacga tcatccacgt
    24241 gccgtcaggc gcaagcgagt ctttgacatg agcggccgcg ccgacggggt ctcccatgtc
    24301 atgcaaacaa tcgaagaacg tgacgaagtc gtagttatct cccggataat ctttagcctt
    24361 ggcgactgcg aactccacgc gttcagccac ccccgcctcg gccgccttct gccgcgccat
    24421 ttcgatggac ttgtcgtgat aatcgaaacc cacgaatgta gaattgggga aagcctgcgc
    24481 catcaggatt gtcgaagcgc caagcccgca ccccacgtct gccaccttcg cccctgactt
    24541 tagtttagcc tcgactccgt caaaagcagg gatccatgcg ttgaccagat tggcggcata
    24601 accggggcgg aaaaatagct cagtcccacg aaacaatccg tgatcgtgct gatgccagcc
    24661 gaagccctcc cccgtgcgaa atgcttcggt aatcttcggc actgctctca gcgaagagac
    24721 ggccagcaaa aaagctcccg gtaaatatgc cggactctgc tcatcagcca gagcgaaggc
    24781 ttgctcctcg gtgagaaagt aacgctgcgt ctgggcgtca tattcaacgt aacccccggc
    24841 cgcctgtgag cagagccact cgcgcacgta acgctcagtc gtgttggttc gctcggccag
    24901 ctcggcggaa gtgagcggtt gctccgcctg agccagcgcc ttgtaaagac cgaggctttc
    24961 gccgatcacc accagaccag cgtggagtgc cgcgccgaag tcggcgacga actttcctaa
    25021 caattgattc aatttttctt cgttgatgga cattcaattc tctcctttgg cgggcaggta
    25081 atcaggcgga atgcttttta taacaaagcg gcccgagcgt caacggccgc gcgcggcaaa
    25141 tagtgtccta atttcagttc tctgcgcgtc ctcggcgtcc tccgcggctc ggcggtgaat
    25201 cacggctgaa ggccactcac cgcagagtcg ccgaggacgc cgaggacgcg cagagaagaa
    25261 acattcaatt caggacacaa cctacctgcg agtcgcgcgc atgaagacat aggaaaggcg
    25321 ggcggaagcc gttcaagctt ccgcccgctg gtgttcgtcc gtcgtcgcgg ggcgtgtgtg
    25381 gtggccgctt cagtcggaga gcttgaaggc ggccatctcg ttggcgttgc ggacgaggag
    25441 gcggccgtcg gcaatgacgg ggacgttcca cgtcttgccc tcgacggcct tgaagcgcgc
    25501 gacctccgtg tgcgccgcgg gagtggcgcg cacgaggacg acatcgccct cctcggtcgt
    25561 gacgatgagg tgtccgctgg cgaagatcac ctgcccgtag ccgtagcgcc cgcccttcca
    25621 cttgcgctcg cccgtgttca cgtcgatgca ggccatgatg ctctcgtcga acccgtagac
    25681 gtggccgtcg tgatagacgg ggctgttgaa cttggccttg agcgacgtgt tctcccagac
    25741 ggcgcgcgcc gtgtaggtgt tgccggagcc ggccacctcg acgagcgccg aacccttgcc
    25801 gtagccggag gagatgaaga cgcggttggc cgcgacgacg atgggctgcg agacgttgat
    25861 gccgtagccg ttcgaccacg ggtgctccca caacaccgcg ccgtcctcgg gcgcgaggcc
    25921 cgcgaggcgg cgcgccgtga cgacgacgat ctgccgccgg ccggcgagcg tcgtgagcac
    25981 cggcgacgtg tacgacgcct gatcgctcat cgagttccag acgcgcgcgc ccgtcatctt
    26041 gttgtaggcg acgaccgtct tgccggaggt tccgcccggc atcacgatca ccttgtcgtc
    26101 aacgatcaag ggcgcggccg cctgccccca ctggatgttc tgcgcgccgt tgtcggagag
    26161 gatgttcttc gaccagatgg cgcggcccgt cttcgcgtcg agacagcgaa actcgccggt
    26221 ggccccgagg gcgtagaggc gtccgtcgtc gaaggtgggc gtggagcgcg ggccgtcgcc
    26281 ggtgtcgtcg gggctgaagg tggcgttcca ggcgttcgtc cacagctcgc ggcccgtcga
    26341 ggcgttgtag gcggcgacga cttcctgacc gcgccgctgc tcgatggtgt aggccgtgcc
    26401 gtcggcgacg acgaaggagg cgtagccgcc gcccacgggc tgcttccaaa ggagctgtaa
    26461 cccttcggcg ggccagctcg tcttcacctg ccgctcttca tagcggccgt cgcgcgaggg
    26521 gccgcggaag ttagtccagt agcgacgacc gccgccggcg gctccggccg cgggcgccgt
    26581 ggccgcgccg gccgccgtgg cgggctgctc gcccgcgggc gcgcccggtt gcgccgccgc
    26641 gccttccacc gcgggctggc cctcgacggg cgcagccgcc gctgcctgtt cggccggggc
    26701 tgcctgcgtg ccgtcggtcg cggcttgctg ttgctgcgcg gcgcggtggc gttcgagttc
    26761 gtcgtagtgc gcctgcgagc cgggcgcgcc gccccgcgtg gcaaagaagt aagcggcgac
    26821 ggccgcgaga gcgacgacgg agacgagcgc gatgatgacg ggcgggcgtc gataccaggg
    26881 cgcgggcttg ttgaaggact cttcgctcag cattttaaat tccctccgat tacgagcaag
    26941 gactaacagt accgcgagcg gtagcgagcg ggtgcgcctg cggcgaaagt ctcgccccgc
    27001 gcacgctgac ccgctcgcta ccgctcgcgg tactgttttt catttcatcc ttgccttact
    27061 tgccgatggc gtacaggttc ttctcgccgc ggatgaaaat cttgccgtcg gcgatggccg
    27121 gcgaggcgta gaccggctcg cccaccgagt tagtgcggac gaccgagtgg acggggccgg
    27181 ctttgatgac gaaggtgtcg ccgtcctcgc tcgtcagcag aatcttcccg tcgtaggcga
    27241 cgggcgaggc ggtaaacgat gaggggatgg gcacgcgccc gccctcgtag acaactttgc
    27301 cggtcttggc gtcgagcgcc gtcatgatgc ccttgtcagt gagcatgtag aggtgctcgc
    27361 cgtagagtat cggcgagggc acgtaggcca cgcccttctc atgcttccac gcgatgttgg
    27421 gcgtgcccgt caggtcgccc gtggcgccga ggcggatggc gtaggcgatc ttggcgggga
    27481 agccggccgt gatgaagacc atgtcggagg tggcgacggg cgaggggatg gcgttgctct
    27541 ccagtccctt cgagcgccac aactccttcc ccgtcgccgg gtcgtaggcg atgatcgcct
    27601 cgttgccgct cgtaatcaat tccgtgcgct tggccgtctt cacaagtatc ggcgtggccc
    27661 acgtcacctg cactttgcgc gggactctcc agacttcctt gcccgtcttt ttgtccagcc
    27721 cggcgatgaa cgacttctcg ccgttctcct cgtcggcctg aatgatgacg aggttctcgt
    27781 ggaggacggg cgagatgccc gtgcccatgc cggccgtggc cagcttgccg aggtcggcct
    27841 tccacgcgag cttgccgtcg aagtcgtagg cgtagagacc ttcggcgccg aagaaggcgt
    27901 agacgcgttg cccgtcggtg acgggggtgg aggcggcgta ggaactcttg cggtggcggt
    27961 cgtcgtaggg cgtgccctcc cacgccgtgc gctcccagag tatcttgccg gtgtcgcggt
    28021 cgagcgcgat gaccttgaag gcgtgctggc ggtcggcgcc gaccgagtcg gggtgcttag
    28081 actccttgcc gccctcgtcc agatgcggga cggccttcgc gccggggacg accgcgcctt
    28141 cgacggccgt cgtcaggaag acgcgcttgc cccagatgat cgggctggag tggcccttgc
    28201 cggggatggc cgccttccac ttgatgttgc gctcaggcgt ccactcctcg ggcagatttc
    28261 tctcggtcga gatgccgagg ctggccgggc cgcgccactg cggccagttc gcggggtcgg
    28321 ccgccgcgcc ggtcgccgag agcgcgagca gggaaaatag acagatgacg agagcgatgc
    28381 tggtacggat tgcgagtttc acggtgcgat ttcctcgggc tgtaagttta gacggcacgg
    28441 ggcggagttt tgttccgttt ccgcgtgatg tttggctcgt tacttgagcg ttgggcgaac
    28501 tggactcacc gcagaggcgc agaggacgcg gggaagcaag gatgaaggat gaaggatgaa
    28561 ggatgagggg aagacgacct gtcttttttc atccttcatc cttcatcctt catccttgcc
    28621 tctgcgcctc tgcggtgaat attgcccgcg tcaaactcaa gtgaaagtta attacctgac
    28681 ttcgagacct tgtccttcgc cgctccggcc tcgccgaagg cgtagacgtg tttgagggcg
    28741 cggacgatga cgaggccgtc ggagatggcc ggcgtcgcca tgatgacctc gccgaccggg
    28801 ttgatgccta agaactcata cttcggcccg gccttgacga caaagacctc tccgtcctcg
    28861 ctcgaaaagt agagcttgcc gtcggccgcc accggcgagg ccgtgaacgc gccgcccttg
    28921 ccgccgaccc gctcctgata gagccgctcg ccggtcttcg cgttgtaggc cgtcaccacg
    28981 ccctgattcg agatcgtgta gagatgatcg ccgtagacga tgggcgtcgg catgtacggg
    29041 ccgccgcgct gcttgctcca cgcgatggaa tcgctcgact ccttgccttc cttgagcgtg
    29101 atgtcgccca tggcgccggg tttgatggcg tagatgggct ggatggggcg gtagccgctg
    29161 gtgacgtaga tgaggtcgtg gccgaagatg ggcgtcgggg tgatgacctc ggagttgggc
    29221 gtcagacgcc acagctcttt gccggtcgcg gggtcgtagc cgcggatggc cttcgtgccg
    29281 ttggtgacga gttccgcgcg cgtcttgccc tcgacgacgg tcggcgtgct ccaggaggga
    29341 atctcctcgc gcggcgtcat ccacaggcgc ttgcccgtct tgatgtcgta ggccgcgatg
    29401 aaggaatctt tctggatgtc ggcctgcacg atgaccatgt tcttgtagat gatgggcgag
    29461 gagccgtact cccactggta atcggggtcg tagaaccagc cggcgtcgag cgtgccgagg
    29521 tcttgcttcc acaaaagctt gccgttcagg tcgtaggcgt acaggccctc ggagccgaac
    29581 agcgcgacga cgtatttgcc gtcggtggcg ggcgtcgccg cggcgtgtgt tgacttggga
    29641 tggcgcttca ccttgggcac gccctcccat gccgcgcgct cccacagcac cttgccggtg
    29701 cgcttgtcga gcgcgtagac cttgtaggtg tgcttcgagt cgtccttgac cgggtccacg
    29761 tcgccgtaga gtccgaagcg cgtctcgtct ttagcggcgc cgctgatggc cgtgatgacg
    29821 aaaactttgt cgccccacac gacggggctg gcgtgcgaga gtccggggat cggggtcttc
    29881 cacgcgacgt tctgcgactt ggcggcgtcc cacgcggccg gcgtcgcctt gccgtcggtc
    29941 acgcccgacg cgctcacgcc gcgaaactgc ggccagttct ggttctgagt ctgcgcgcgg
    30001 gcgccggcag agagggcgct aaagaggcag aggagggaaa gtgcgaatct tttcatggga
    30061 aggaacctct cggaaagtcg ggagtcagga gtcaggagtc aggagccaga aggaagaaag
    30121 caaccatgct tttattctga ctcctgactc ctgactcctg acttcactgt tgtttcgttt
    30181 cttcgaccct cgtgtaaggg atggtggccg gcccctgctt gagcgtgaag ccgttgacct
    30241 tgccggcctc gggcgtgaag ataacggtga tgccgtcgaa ggcggtcggc ttgaaggtca
    30301 tcttgtcgag ggcgtacatg gtgagcggcg gctggcccgt ggccgcggcg atgagcttgc
    30361 cgtccttgac ggagaagacc agctcggggc cttgcgtgcc cttgaagcgg ccgacgtatg
    30421 tctggagcgt cgcggcatcg acctcggcgg gcggctgcgc gccggccttc ttcaacatct
    30481 cgacgatctc gactccctta tcgccggcct ccgtcgccgc gacgaacgcg cccgtcatcg
    30541 actccggctt gagattgccc ttgtcgagcg cggcgcggac gagcgcggcg tttccgcctc
    30601 gcgcccccgt ggaaagcacg tcgcccgcgt cgtcggcgct cttttcaagg atggcgcgga
    30661 cggcatcgac gtgccccttg tcgagcgccc acgtcatcgc cgtcgcgccg tagaaagtat
    30721 cgcgcaccgt cacatcagcg ccgcgctcga tgagcagttt gacgatgtcg gcgttgccgc
    30781 gctcggcggc cttgaacaga gccgtcgcgc cgtagcggaa tttggcgttc acgtcagcgc
    30841 ccttgtcgag cgtggccttg acggcggcca cgtcgccctt gcgaacggcc tcgtagagtt
    30901 catcgttgag cttttgtttc tccggcgacg gtgtcgcggc cggcacgggc gtcggcgtcg
    30961 cggtcggtgc tggcgacggt gtcggcgtcg gcgtcgcggt ctgcgcgatg gcggccggcg
    31021 gggcgagcag gagcaggagg aggaggggca gaagtcttct gagtttcata aatcgtaact
    31081 ccccggtttt tcggagtcgt ccggtaggtg gtaaattaaa ggagcaacca gtaaggctcg
    31141 cgcccgtcgc gggccgcaaa acgcggagcc gacgtaagcg ccgcttgtcc cggccgaaag
    31201 cccggcgtat ttaaactgtg ttcgggcctg aaagcaaggc cgccgaaagt cgcctcgggc
    31261 gcgggccgaa ttctacacgc gtagaaacgc aggcgagcgg ctaaaggttt ctttttacag
    31321 cctttttaca acgaacacgg ttcgatgcgg cataatactt tggtcactga aacaaaccgc
    31381 cggcgtgcgg cgtctaaact tcgggcgagc ctgaatgagt agcggccccg gcgggcgcgg
    31441 aaggcaagcc cgcgagccgt ctcccgcaag ctctcccacg aaccgaaatc aaatacccga
    31501 gaacttagaa gatgcgtgca atgttgttct ccagacgttt cgtcctcctc gccctttcaa
    31561 ctcttctgct cgcgccggcc gcactcgcgc agaaactcac cggccccgtc gattgggcgg
    31621 agtggcgcgg ccccctgcgc gacgggcgct cgttcgagaa gaatctgccg tcgaagtggt
    31681 cgccggcggg cgacggcctg ttgtggaagg ccccctacgg cgggcgctcg acgccggtcg
    31741 tcttcggcga ccgtctctac ttgcagaaca cggccgagaa gggtgagcgc gagcaggagc
    31801 gcgtgatcgc gtttaacgcc gacacgggca agctcgtgtg ggagcatcgc ttcaacgtgt
    31861 acctgtcgga cgtgccggcg catcgtgtcg gatgggcctc gcccgcggtt gacccggcga
    31921 cgggcaacgt ctacgtgctc ggcggcgggg gcacactgct cgcgctctcg gggcgtgacg
    31981 gaaaagtttt atgggagcgc acgctcggcg aagacttcgg cctgctgacg acgcacggcg
    32041 ggcgcaccat gtcgccgatt gttgacggcg acttagtcat cgccagcggc gtgatgttcg
    32101 tctggggcca gcacgggcaa ggccgacacc gcttagtcgc tttcgacaag cggacgggcg
    32161 aggccgtctg ggtgagcgcg ccgggcgggc ggccctacga cacgacctac tcgccgccgc
    32221 tcatcgccaa cgtggcgggg acgcgcctgc tgcttcaggg cggctcggac gggcaggtct
    32281 acgccgtcaa gccgcagacg ggcgagttcg tctggaagta cgaaatctcg aagcgcggcc
    32341 tgaacacggg cgtcgtcgtg tcggggacga ccgcatatct cacgcacagc gaggagaacc
    32401 tgaattcgag cgagatgggg atgctcacgg ccgtggacgc cacggccaag ggcgacatca
    32461 agccggagca cgtgaagtgg acggtgacgg gctggcaggg cggcttctcc tcgcccatcc
    32521 tcgacggcga acgcctctac cagatcgaca acggcgccaa cctcggcgcc ttcgacacca
    32581 acaccggcaa gcagctctgg ctccagaacc tcggcaccat ccagaaggct tcgcccgtct
    32641 tggccgacgg caagctctac gtcggcaccg agaacggcaa gttcttcatc atcgagccgt
    32701 cggcgacggg cgcgaaagtt ctcgacgagg atcagctcgg caccgcggcc ctgcccgagt
    32761 ccatcatcgc ctcggtcgcc gtctcccgag gccgcatctt cctcgtcacc gacgcggcgc
    32821 tctacgcctt cggcaagaag tcgaacgccg cgccgccgcc gctccccggc gaccccgtca
    32881 gcggcaactc caacccgacg gtcgcgcacg tgcaggtcgt cccgaccgag ctgctgctgc
    32941 ggcccggcga caaggcgagc ttccgcgcgc gtctcttcga cgagcgcggc aacttcatgc
    33001 gcgaagagaa agcgacgtgg acgctcgaag gactcaaggg cgcggtcacc gaaacggggc
    33061 agttcacggc cgcgggcgac gcggcgcagg ccggtctcgt caaagccacg gtcggcggcg
    33121 tcgcgggcgc ggcccgcgtg cgcgtcatcc cgccgctgcc gtgggccgag aacttcgacg
    33181 cgctggagcc gaacgccagt ccggcgcact ggacgaacgc gacgatgaag ttcgccgtgc
    33241 gcgagatgga gggcaacaag gttctggtga agacgaccga ggggtcgtcg ctcctgagcc
    33301 gctcgcgcgc ctacatgggc cagcccgact tgcacgacta cacggtcgag gccgacgtgc
    33361 gcggcgccga gcgccgtcgc acgatgggcg acgccggcgt catcgcgcag cgctacgccc
    33421 tcacgctcta cggcaacggg cagaggctca gcatcgagcc gtggcagccc gaagtcgaac
    33481 gcactgcctc cgtccccttc gagtggaaga aagacacgtg gtatcgcatg aagctgcggg
    33541 tggagaacct gcccgacggc cgtgtgcgcg cgcgcggcaa ggtctggctc acgggccagc
    33601 cggagccgga ggcgtggacg attgagaagg tcgatgcgct cccgaaccgc gtgggcgccc
    33661 ccggcatctt cggcaacgcg cttgccgaaa tctacttcga taacctgaag gtgtacgcca
    33721 acaactagaa tgaacgaaga cgcacagcac gcgacgcagg cgacgacgcc acagcaacaa
    33781 cagcaggggc cgacgcccga gcgcatcttt ctaacgctca ccggctatca gcaggccgcc
    33841 gccctgaaag ccgccatcga gctggatatc ttcagcgcca tcgccgaggg cgacgacacc
    33901 agtgagaagc tggccgcgcg cctcccggcg gccgagcgcg gcgcgcgcat tctctgcgat
    33961 tacctgaccg ctttgcagtt tctcgccaaa gaggacgggc gctaccagct cacgccggag
    34021 tcgaagttgt ttctcgaccg ccgctcgccg gcctacgtcg gcggcgtctc cggcttcctc
    34081 tcgcggcctt acatgatcga ggcgtttcag aacgtggccg aatgcgtccg caagggcggc
    34141 acgatgctcg aagatcaggg cacggtgacg catgagaacc cgatctggga agacttcgcg
    34201 cgcagcatgg cggcgatgat gcagccctcg gcacaggcca tcgcgcgcat cacgggcgcg
    34261 gccgagatgg aagagtgtaa ggtgctcgac atcgccgccg gccacggcat cttcggtatc
    34321 accatcgccg aacagaaccc gagggccgag gtctacgccg tcgattggca caacgtcttg
    34381 cagattgcgc gcgagaacgc cgccagcgcg ggcgtcgcct cgcgctatca catgatgccg
    34441 ggcagcgcct tcgaggtgtt gttcggcgac gactacgacc tcgtgctctt taccaacttc
    34501 tttcaccact tcgacccgcc gacctgcgag gcgctgatgc gaaaagcgca cgccgccctc
    34561 aagccggggg gccgcgccgt gacgctcgaa ttcgtgccca acgaagaccg cgtcacgccg
    34621 cccgcggaag ccgccttcgc catgaccatg ctcgcctcga ccccttcggg cgacgcctat
    34681 acgttctccg aattcgacca gatgttccgc aacgccgggt tcgcccgcag cgagctgcac
    34741 ccgtcgccgc cggagcacat catcgtgagc acaaagcagt gagcagtgag cagtaacaca
    34801 gaggaattga agtcaatgaa aaatattcga cgcagtagag tcatcattct tttaacggcg
    34861 gtgttcgcgg cggtagcggc aggcggttta ctgcttactg ctcacggctc actgccgaac
    34921 ccttcagacc ccggcaccgg cgactggccg atgtggggcg gcacgcccga ccgcaacatg
    34981 gtcacgagca tgaaggggct gcccgtctcg tgggacgtga agacgaagaa gaacgtcaag
    35041 tggatggcgc aactgggttc gcagacctac ggcaatatca cggtggcggg cggcatcgtc
    35101 ttagtcggca ccaacaacga gggcgcgcgc gatcccaaga tcaccggcga caagggcgtg
    35161 ctcatggcct tcaaggagtc cgacggcacg tttctctggc aggccgtcca cgacaagctc
    35221 gtcgcgggcc gcgtcaacga ctggccgtat cagggcatcg cctcctcgcc cctcgtcgaa
    35281 ggggagcgcg tctactacgt ctctaatcgg ggcgagttga tgtgcgtcga tgctcagggg
    35341 ttccgcgaca aggagaacga cggccccgtg accgacgaga agctgacggg cgagaccaac
    35401 ccggacatca tctggcggct cgacatgatg ggcgacgagg tcgggagctt cccgcacaac
    35461 ctctgcaact cctcgcccgt ctcctacggc gacctcgtct acgtcagcac ctcgaatggg
    35521 caggacgaga gccacgtcaa catccccgcg ccgaaggccc cggccatcat cgccgtcgat
    35581 aagaagaccg ggaaagtcgt ctgggaggac gccagccccg gcgacaagat tctccacggg
    35641 cagtggtcaa cacccacggt cggaaaaatc ggcgacaccg tacaggtcat catgggtcag
    35701 ggcgacggct gggcgcgcgg ctacgacgct ttgaagggca cgaagctctg ggagttcgac
    35761 ctcaacccga aggactccgt ctggcccaag acgcgcaacg agatcatcgc caccccggtc
    35821 atctacgagg gcaagattta tctcgccaac ggacaagacc cggagcacgg cgaaggcgtc
    35881 ggccacatgt actgcatcga cggcaccaag cgcggcgaca tcacgaagac cggcatggtc
    35941 tggcactacg acaagattcg ccgcaccatc tcaacgccgg ccatcgttga cggcatcatc
    36001 tatcaacccg acttcagcgg cttcctccac gccatcgacg ccaagaccgg acaggcttac
    36061 tgggtacacg acatgttcgc ggccatgtgg gggtcgcccc tggcgtcggt cgcgggcggc
    36121 gtgctgtatc tgggcgacga ggacggcgac gtggtcgtca tgcagttggg gaagacgaag
    36181 aaggtgctgg ccgagatcaa catgggaagc tctgtctaca gcacgcccgt gccggccaac
    36241 ggcgcgctct acatcgccaa ccgcaaccag ctcttcgcgc tggccgaggg ggccggcggc
    36301 gcgcccgcca cgcccaagcc ggcgacggcc tcggccacgc cggccaaccc gtagcttgaa
    36361 tgtggatcca tagcttgaat gtatagagtg gggcgttcac cgcagagtcg caaagcacgc
    36421 agaggggcgc agagacttga atcaactctc tgcgccctct gcgcctctgt gtactctgcg
    36481 ttaaatgatt ttcctgaaca cacacgccga cgaactctgt aacgagttcc acgtctgaac
    36541 gtgaccttca tgcaatcttt cgacttccag ccgcgcacgc gcgtgatttt cggcgagggc
    36601 gctgtcgcgc gtctcggtga ggcggcgcgc gagctgaact ttcggcgcac gctgctcgtc
    36661 gccgatcatg gtctcgtcgc ggccggacac gtcgggcatg cgtcggagat tttgtcggtg
    36721 gcgggcgttg aagtagtcgc gttccacgag ttcgagtcga acccggacac ggcgatggtc
    36781 gagcgcgggc gcgcgttcgc ggccgacgcc ggtgtggatt ccatcgtcgg cctcggcggc
    36841 ggaagctcgc tcgactgcgc gaagggagtc aacttcctcc tgtccaacgg cgggcagatg
    36901 cgcgactacc tcggctacgg ccgcgccgcg cgacccttgc ttccgatgat cggcgtgccg
    36961 acgacggccg ggacggggag cgaggcgcaa agttacgcgc tcatctccga cgccgagaca
    37021 catgccaaga tggcctgcgg cgaccccggc gcttccttcc gcgtggccat cctcgacccc
    37081 gcgctcacta tctcgcagcc gcgccacgtc acggccaccg ccggcctcga cgccgtcgcg
    37141 cacgccgtcg agtcgttcgt ctcgacgcgc cgcacgcctg tctccgaagt cttctcacgc
    37201 gaggcgtggc ggctgctcga atcatcctac gagcgcgtgc tcgccgtagc cacggacgtg
    37261 gaggcgcgcg gccggatgca gctcggcgcg cacttcgcgg gcgcggccat cgagaactcg
    37321 atgctcggcg ccacgcacgc ctgcgccaac ccactgacgg cccgctacgg caccgaacat
    37381 ggcgccgcta tcgccgccct gctcccgacg gtcgtgcgct ggaacgcatc ggccgcgggc
    37441 gagcgttacg cggagttgct ccacgcggcg cggcggggcg gggataatga atcgaacgcg
    37501 gcggaggcgc tcgcgcgccg gctcgaagag ttgatcgcgg cgggcggact cgcgtcgcac
    37561 gtccgcgccg tcgtcgcgag ccgcgaagac ctcgcggccc tcgccgaaga cgcggcggga
    37621 cagtggacgg gacgcttcaa cccgcggccg ttcgaccggg cgggcgcttt ggaagtttac
    37681 gagcgggcgt actagattag ttttcgcttg agtgtaagga aggttgtact caccgcagag
    37741 gcgcagagga cgcagagggg gcgcagagag ttgaagcgtt tagagggagg gaagatgcgg
    37801 gaagttgaaa gtgaagaagg gcaggagacg ttggcgcgtt caatctcgcg gcgcgggttc
    37861 ctcgcggtgc ccgtgggtct ctacctcgcg ggccatgcgg cgcgcgcgca ggagttcacg
    37921 ggcggcgaca gttggccgca gtttcggggc aacccggcgc tgaccggctt tacgcgctcc
    37981 ggtctctcgg acacgttgcg cgtgctgtgg acttacgagg cgggcgagtc ggtcgagtca
    38041 tcggcggcga ttgtgggcgg cacggtctac gtcggctcgc agaaggggga gctggtggcg
    38101 ctcgatttgg gaagcgggcg cgagcgttgg aagtacgggg ccgggtcgcc gataggcgag
    38161 tcgtcgccgg cggtcgtcgg cggcacggtc tacgtcggcg acctcggcgg cacagtccac
    38221 gccgtctcgg cgcgcgacgg gaagcgcgtg tgggttttca agacggggag cgaggtgaag
    38281 tcgtcgccgg tcgtaagcgc cggcaaggtg ctcgtcggct cttacgatca gcacttgtac
    38341 gccctcgcgg cgcgcggcgg aaacgtcctc tggaagatga agacggacgg cgccgtccat
    38401 tgcacggcgt cggtcgtggg cggcgtcgct tacatctcgg gttgcgatga gcgtctgagg
    38461 gccgtgcgcg tcgccgacgg ccgacaggtc ttccagctca actcgggcgc atacaccggc
    38521 gcctcgcccg ccatcatggc cggcaacgcc ttctacggca ccttcaacaa cgacgtgctc
    38581 ggcatcaacc tcgtcgccaa gcgcgtcggc tggcgctaca cgaacccgga gcggcagttc
    38641 ccgttctatt cctcggcggc ggccgtggac gcgcgcgtcg tcgtcggcgg gcgcgacaag
    38701 gccgtccatt gtctcgacgc gcgcaacggc gctcgactct ggagcttcaa cacgcaggcg
    38761 cgcgtcgatt cgtcgcccgc gctcgccgcc gggcgcgtct tcgtcggctc gaacgacgga
    38821 cgcctctacg cgctcgactt cttctccggc gacaaggcgc aggagttcaa cctcggcggc
    38881 cccgtctcgg cctcgccggc cgtcgccgcg ggccgcgtcg tcgtcggcac gcaggacggg
    38941 cgcgtttact gtttgggatg agtcagtaag cattaagcct caacgagcgc cggaggcgcg
    39001 gaagaatgta gcccacggcg tcagccgtgg gttttaagcg gcaaccaaaa tcaaagcccc
    39061 ggaggggcga aagaagtgca gcgcgcgtct aacggcggtc gcaatttctt tcgcccctcc
    39121 ggggcttcat ttcctttgcc gggcctgtcc ccacggctcg cgccgtgggc tacactcttc
    39181 cgccgctttc agcggctcgt cgtcttctta ctgcacatca ctcacgcctt cttaacgaag
    39241 acgcgcttgt tgttgaaggt gccgcgcggg gtctcctgga tggtctcgat ggagatggtg
    39301 ccgtcgggcc acgccgtcca cttgcgggcg atgatgaggg tcacgtcctg cccctgcggg
    39361 ttcttggtct gaatctcctc ttcgaggaag aagcccttgc cgttggggag ccagttgcct
    39421 ttgcgcttgc ccttggcggg cggcgcgtcg gggcgcggcg gcggcggggt gaaatcgatc
    39481 tccttgccgt cgagcacgaa ctcgtccttc tgtacgcgct cgccctgcgc gttgacgatc
    39541 ttgacctcta aagagacctt gtcgccgttg tggacgaccg tcatcgtctg cttcatgtcg
    39601 ggcgggatac tgtggctttg cttctggtcg agttcccatg tgccgctgaa gttgggccgg
    39661 gggccgaagg ctttcgccgc gaccaccgcg gcgagcaggc aggcgagggt aacgaggggg
    39721 gttcttagtc tcatcgcttt aatctcctga atgacacctg attgtgggag caacagtacc
    39781 gcgagcggta gcgagcgggt ctgacgcacg cgtggcgagc ctctcagtac agccgcaccc
    39841 gctcgctacc gctcgcggta ctgtggaaaa cggccgcggc gggcagctac ttcttctgca
    39901 actcggcgag cgccgccagc acctcttcgc tgtggacgct cggcttgacc ttgaggaaca
    39961 cgcgcgcgat cttgccctcg gggtcgatga tgaaggtgtt gcgcgccgag tagttggccc
    40021 cgttgtattc catcaccgag ccgtaggcgt tcgacacctt cgcgtcgatg tcggcgagca
    40081 gcttgaagtt gaggccctcc ttggcgcaga actccttgtg cgagtcggcg gtgtccacgc
    40141 tgacgccgag gatgacggcc ttcgcctgct catacttctg taggtcgcgc tggaaattgc
    40201 gcgcctgcat ggtgcagccg gaggtgaagt ccttcgggta gaagtagagc acgacccact
    40261 ggccgcggaa atctttgagg gccgcctgct tgttctcgtt cgtggtgagc tggaagtcgg
    40321 gagccgccgc gccgaccgcg ggcggatccg tggctgccgt ggcgatggcc gacgcggaga
    40381 gcgtcagcgt gacggtggcg atggcgagca gtgtaagaat gcgcgttgtc agcagtttca
    40441 ttgggaatcc cttttgtctt gtttgttgtt caggcggttt gtttttcggg gaagtatttg
    40501 gcgatggtcg cggcagccgg cttgcgcgtg acgagcgaga cgaagaacat caggagcgcc
    40561 gagccgaaga ccatcggcac cacgggcatg aagccgtaga cggtcgtgcc gcccggcgtg
    40621 cgcgccagca cctcggtcgc gccgatggca taggcgaccg tgttgcctcc cggcgcgggt
    40681 gcgggcacga ccgactggaa gatggcgacg gcgatgacgg ccgagatcgt ccagacggcg
    40741 acggcgagcg ccccccactt cgtgctgccg cgccagaaga gcgcggcaac gaggagcggc
    40801 gagagcgagg cgaagccgga gaaggcgtac tggatggcca gctcgaagat cgactcgggc
    40861 gcgcgcagcg ccacggcgta ggcgaagatg gtcaggagga tgacgaagat gcggcccgtc
    40921 tgcacctgca cggcctcgcc gaagcgacgc ttgccgccgt agaaggcgaa cacgtcctcc
    40981 gtgaacatgg tggagagcgc gagtatctgc gagtcggagg ccatgacggc ggccatgatg
    41041 ccggcgccga gcaggccggc gagccacagc ggcgcgaagc gttcgagcag cagcaggatc
    41101 acgtcgtcgc cggccgcctg ctcgcgcagc tcgtcgcgcg cctccggctc catcgtcttg
    41161 ccctgagtcg ccagcacccg gcgcgcctcc tgcttggcgc gaatcgcggg cacgtccgag
    41221 acgcggttgg ccatcacgcc caaaaagacg cacggcagcc agatggcgag gatgcagagc
    41281 gggtagagga tgaccgtttt cttaaactgc accatctttt tggccgtcag gcagaagatg
    41341 gcgatgtgcg ggaaggcgat ggccgagagc gggatgaagg tgtagctgaa gaagtagtag
    41401 ggcgagatgc gctcgcgcgt gagcaggggc tttaaggccg gcgcttgctc catcgcctcg
    41461 acggccgcgc ggaagccgcc catctgcgag ccgatgacgg agacggcgat ggcgccgaag
    41521 acgaggaaga gaatcgtctg gaacgtgttg acccacgccg tcccgcgcat cccgccgaag
    41581 aagacgtaag acatcacgac catcgcgacg atggcgccgc cgaaccagaa ggggactaac
    41641 ccgccgctga tggccgacag cgtcgtgccg ccgcccatga tgccgatgat gatgtagggg
    41701 acgagcagca cggcctgcac ggcgaagatg acggtgccga tgtgtccaca ctcccagcgg
    41761 tcgcgcatca tctgcaccgg cgtcataaag ccgtacttct tgcccagcgc ccagatgcgc
    41821 gtgccgatga ggaagatggt gagcgggatg acgagtcccg acgacgaggc catcaacccg
    41881 taggtgacga tgccgttcga gaaggcgtgg cccgaggagc cgaggatggc gaaggccgtc
    41941 atgttcgtcc cgaacagcga catcaggaag acgaacgtcc ccaacgagcg gttggcgagg
    42001 aagtagtcct cggccccgtg gtggccgcgc gagcgtcgga aggcgaagac gccgatgtag
    42061 acgacgacgc cgaggtatat gaagacgacg attgccggga tcaactagaa gctcctatga
    42121 ataggtgata agtgataagt gataagtgat aagaaagaat ttgtcttctc ttatcactta
    42181 tcacttatca ccttccgtgc cacttcttct tcgaggtggc cgggccaggc gaaggtcacg
    42241 agcagccaca tcagcccggc gcacgccacc gagaagcacg cctgatagaa gaggccgatg
    42301 ggcatgaagc cgaagacgaa ggggcgcgcc tctctccagt tccagaagtc ctggtgcagc
    42361 acgtagagca cggcgacggc gatgacgagc agcagttttc tcatcgttct ttacaatggc
    42421 ggcgggcgcc gcggcgttgg acgcgctatt atcatcgcag acgcgaaagc cggcaacttt
    42481 tttattcgcc gtttcgccgg atatgatgta aaaaggtcgt aaaaatagtg tcctcgccgg
    42541 aacgacttcc ccggttaacc acgaggccgc cgccccgacg aacgaaaggg agtgtgaccg
    42601 ccgtgcgcct gatacacaga atctttctcc tgctggccgc gttcgtcatg agcgccgtga
    42661 gtgcgtcggc gcagaagacc acgccgccgt ctcaaaccgc gccgcctgcg tcgtcgtcgg
    42721 cggccgcgac aaccccgtcg gacgacgacg acgaagtcga gcgcgtgagc gtccacgtcg
    42781 tctcggtgcc ggtgtccgtg accgaccgcg acggccgctt cgtccccgac ctcaaggagt
    42841 ccgacttccg cgtctccgag aacggccgcg accagagcat ctcttacttc gcctccgtcg
    42901 agcaaccctt cagcgtcatg ttgctcctcg acaccagcgg ctcgaccagc atccgccttc
    42961 gggacatcca aacggccgcc gtcgaattca tcaaccagct ccgcccgcaa gaccgcgtgt
    43021 tgcccatcgc cttcgacaac gacgccgtgg cgctcctgcc cggctgggcc agcgaccgcg
    43081 ccgtgctcac acaggccatc cgctccgcgc gcacggggat gatcatggag gacaaggatc
    43141 gcgaggtgaa ggtcggctcg gacggcaaga aggtcgtctt ccagcacatc aacacgcggc
    43201 tctacgacgc cttctacaag tcggccgaaa cgctccgaca tatcaagggc cgcaaggctc
    43261 tcattctctt taccgacggc tacgacaatt cgagtcgcct cgcgacacgc gcgagcacgc
    43321 tgacgctggc cgaggaattg gacgcgctcg tctacgtcgt ccagttcacg ccctacggca
    43381 actacgcgcc caaagagcag ccggtggcgg gtaacctcgg ggggccggtc gcgcggccaa
    43441 gcacggcgcc ttcagtgagc gagattcggc agtcgaccac gcagatgacc gcggcgggag
    43501 tgtcgccggc ctcgccgacg cgggcctcgc ccatcggcgg cggcagcgcc agcgtgctgg
    43561 gcgcaaggac gacgagaccg tccgagcagc gtcacccgaa ggcgctggag gtcgaacgct
    43621 atctcgaagg gctggccgag aaaacgggcg gacgcttcta cgtcgccgac gacatgaaca
    43681 agatcaagct ggcgttcgcg gccgtcgccg acgagctgcg acgcatgtac agcatcggct
    43741 atcacccgac gccgctcgcc aaacccggcg agcgtcgcga agtgaagatc aatgtccggc
    43801 gcgagcgcgt cgccgtccgc gcgcggcgcg cctacgtctt ccgtccacgc tgagcgcgcc
    43861 gtctcaaaaa cctcgcagtg aatcattcat ggagttcgat gatgaaaact aaagcctcgc
    43921 ttgcgccgac cctcctctgt ctcagcctgc tcgcgccggc cttcgcgcaa cagcccgtcg
    43981 ctaatccggc gagcgcgccg cgacaacagc cgacccagcc gcggccgcag caggacgatg
    44041 acgacatcgt gcggatcacg tcgaacctcg tgcagctcga cgtgatcgtc acggacaaag
    44101 acggccgccc cgtcaccgac ctgcgccccg aagatttcca gattttcgag gacggcaagg
    44161 aacgcgagat tacgaacttc tcctttgtct cgaccgaagg cccgtcgccc ggccagccca
    44221 cggaggcggg cgctccacag ccggccccac gccggacgga aaaggcactg ccgcccatgc
    44281 cgagcgcccc gctgcatagg agagaggtgc ggcgcaccat cgcgctcgtc gtcgataatc
    44341 tgaggatgag ccacctgagc gccacgtcgg cgcgcgaggg gctgcggaag ttcgtcaacg
    44401 agcagatgcg gccgggcgac ctcgtcggca tctacacgac gcgcggcggc agcggctcgc
    44461 tccagcagtt cacctccgac aagacccagc ttcaccgggc catcgaccgc gtgcgctggt
    44521 atccgccccc cctcggcggc gtgggcgtgg acgtgttcga gcctgcgcgg gacgactcca
    44581 ccttcaagcc gaccacgccg ggctccggcg gcacgcgcct cgacggcttg gccgttggca
    44641 aggaggagtt cggggacgcg gagacgcggc agaggcgaga gcgcatggac gagcgcaacc
    44701 gcgacctcgt cgcctacggc gcgctcggca cgctgcgcta catcatccgc ggaatgcagg
    44761 aagcgccggg gcggaagtcg gtcgtgctgt tctccgacgg gataccgctc ttctcacgca
    44821 atgacggcgc ggcgtcgcgc tcgttcgacg ccatggatcg ggtcatcgaa tcggccaccc
    44881 gcgccgcggt cgtcatctac acggtggacg cgcgcggcgt tcacatcccc ggcatgatca
    44941 ccgcggccga cgacgtgcgc ccgaacgcca cccagagtct ggttgaatcg cgtctcgaaa
    45001 agggagcccg cgccgaggac ggtttgacct acctcccggc caagacgggc ggcctcttcg
    45061 tccgcggcac gaacaacatg gaccgcgcgc tcgaacgcgt gatgcgcgac caaggcggtt
    45121 tctacctgct cggctaccgc cccacggagc agaccttcaa gggcggaagc aaagcctttc
    45181 gtgagatcga cgtgaaggtc agacgctcgg gcgtgagcgt ccgctcgcgc ggtgggttca
    45241 tggccatcac ggacgaggat gcgcgccgcg agacgcgcgg cagcgacaag gagctgtacg
    45301 ccctgcttgc gtcgcccgtc aacgccggcg gcgtttacgt ccgcctgacg gcgcgggtcg
    45361 tcggcgaccc gcgcggaggt gggtcggcgg cccgcatgct cctgcacgtg gacggcaaag
    45421 acctgacctt caccgacgcg cccgagggcg gcggctataa aaaactcgtc ctcgacgtgg
    45481 cggccgtcac gctcggcaag gagggacaga tcatcgacga gttcaaccgc acgcacaccg
    45541 tccgcacggg cggcgagcat ctcgacaaca tcgtccgcca cggcctgacc tacacggccg
    45601 acgtgcccgt caaaaagccc ggcacctacc aactccgcat cgtcgtgcgc gatcaggcgt
    45661 cccagcgcgt cggctcggcg agccagctca tcgacgtgcc cgacctcagt aagcagcgcc
    45721 tcacgctctc aggactcacg ctgcgcgaga tcgtcgccgg gggcgcacag cagcaatcgc
    45781 cgcccgattc ggcgcggccc acggctgaga cggcgattgc ggccgtcacc agcctctccg
    45841 accctgccgt ccgacgcttc cgtcccggcg ccatcctcgc ctacggctca caggtttatc
    45901 acacgacggc cggcggcggg ccgggcggga cgccgcgtct gacggctcag gtgagcctgt
    45961 ttcacaacgg ccagccggtc gtgaagggcg cgcccaccgc ggtcgagttc gacccgacgc
    46021 ggcccggctg gctcgagggc gcgctacggc tcgtcccgca ggcgaagccg ggcgagtacg
    46081 tccttcaggt cgttgtcacc gacgagtcgg tcaaggacaa ggatcggcga cagaccgcgc
    46141 agtggatcga cttcgaggta atcaactgaa ggccagaggc cggtggatgg acgattacac
    46201 gagcgacaca tacggcgagc gccacgccga catctacgac gagcgcctgc ctgactacga
    46261 cgaggccgcc atcgagatgc tcgtcgggct ggcgggcggc gggcgtgcgc tggagcttgg
    46321 cgtcggcacg ggccgcgtcg ccctgccgct cgcggcccga ggcgtggaag tcaacggcat
    46381 cgacgcttca ccccgaatgg tcgagaggat gcgcgccaag cccggcggcg aggccgtgcg
    46441 cgtgaccttc ggcgatttcg ccggggtcga agccgaaggc gagttcgcgc tcgtctacgt
    46501 cgtcttcaac accatcttcg cgctgctcac gcaggacgag caggtgcgct gcttccgcaa
    46561 cgtcgcgcgc cggctcgcgc ccggcggcgt cttcctcatc gaagccttcg tgcccgaccc
    46621 gacgtttcac cccggcggcc agagcgtcaa ggtcggacag atgaccaacg acttggtcga
    46681 actccacgtc acgcaactcg acgcggcgac gcagatgctc gtgggtcagc aaatccgttt
    46741 cgacggcgcg ggcgcgcgac tctaccccgt acaactccgc tacgcgtggc cctcggagct
    46801 ggacttgatg gcccggctcg ccggcctctc cctgcgccaa cgccatgcgg gctggcgccg
    46861 cgagccgttc gacgcgcgga gcacgaaaca cgtctcggtc tacgcgcgcg agggtgacgc
    46921 cggatgagtc ccacgcgcgc gaaggcgtcc agaggtgtga actcgaaatg gcacaggctg
    46981 agcgacgatg agttgctcga catgcgtctg tgcgatctgc ccgtgagcat ccgcaagacg
    47041 ccgctcgaag ggcgtatcgt gcggctctac tcggaattgg ccgagcgcgg cctgcgcttc
    47101 cgcccgcaca tctggctctc ggaggattgg ttctcgcccg acggcgtgcc cggcttcgcc
    47161 atcccgttct acctcgcgca cccgcgcctc gtgaagcttg agcgcgcgca gatgctcgaa
    47221 gtcgagggcg gcgccgagaa ggaatgtctc gccatcatgc gccacgaggc gggccacgcc
    47281 gtctcgaacg ccttcaacct ccaccgccgc cgccgctggc gcgaggcgtt cggacgcttc
    47341 accgcgccct accccgagca ctacatgccc gaccctacca gccgcggctt cgtcctccac
    47401 ctcggcgcat ggtacgcaca ggcccacccg gccgaggact tcgccgagac cttcgccgtc
    47461 tggctcgcgc ccggctcgcg ctggcgcaag cgttacgtga gttggccggc catgaagaaa
    47521 ctgcgttacg tggacgagct gatgcgcgag atcgcgggcg ctcgtccgcc caacaacagc
    47581 cggcagaagg tcgagcctct ggccgagatc aaaaagacgc tgcgcgagca ctaccggcgc
    47641 aagcgcggcc actactcctt caagtggccc gcctactacg accgcgacct caaacgaatc
    47701 ttttcggacg acgaggccca ccgcgcttcg gccacggccg ccagcctcct gcgccgtcac
    47761 cgtcgcgagc tggggcgcct cgtcgccgac gtgaccggcg tccacaacta cgccgtcgat
    47821 caggtcctga agaacatgat cgaccgctgc aaggagctgc gcctgcgcgt ccccctacac
    47881 gaggagaatg ccaaagagcg cgctctcatc atgctcaccg cgcacacgat gaatatcgtg
    47941 cacgccggct atcaccgcgt cccgctatga cgaagaaaaa aaagccgacc gtcaagaagc
    48001 tccgcattct cgtgctctgc cagcacgacc tcgtcccgcc cgacgcgctc cccgagggcg
    48061 tggcgaagga gacgctgcgc tggcgcaccg agtacgacgt ggtctccacc ttacgctcga
    48121 tgggccacga cgtttacccc gtcggcctct acgacgagac ggacgtgatt cgcacggcga
    48181 tgcaggatca cgagccgcac gtcgccttca atctcatcga agagttcgac cgccagccca
    48241 tgttcgacca gcacgtcgtc agctatctgg agctgaagcg tctgccctac acgggctgca
    48301 acccgcgcgg cctgaccatc gccaaagata aggcgctcac caagaagatt ctggcctacc
    48361 accgtatcaa cgtccccggc ttcggcgtct tcccgcaagg acgcaaggtg cgccggcccg
    48421 cgcgcctgcg ctttccgctg ctcgtcaagt ccgtgacggt cgaaggctcg gtcggcatct
    48481 cgcaggcttc catcgtcaac actgacgaaa aactcgccga gcgcgtcgat ttcatccacc
    48541 ggcagacgaa ctcgccggcc atcgccgagg agttcatcga ggggagagaa atctacgtcg
    48601 gcgtcatcgg caaccgtctg ctgaaggctt acacgccgtg ggagctggag atgaagacgc
    48661 tgcccgaggg gactgtgaac atcgccacgg ggaaggtgaa gtggaacccg aagtatcagg
    48721 agaaagtcgg cctcgtcacg cacgcggccg aggtcacgcc cgagctgcgc cgccgcctcg
    48781 aacatctctc gaagcgcatc taccgcctgc tgtttctctc cggctacggc cgtctcgact
    48841 accgcatcac cgaggacggc cgcatctatc tcctcgaagc caaccccaac ccgcaaatcg
    48901 cccacaacga ggacttcgcc gactcggccg aagactgcgg cgtcaaatac ggcccgctgc
    48961 tccagaaaat cctcaccctc ggcatgagct acgagccggg cgtggtgtaa aaaagcagtc
    49021 aggagtcagg agtcaggagt cagaataaaa acggaggcgc tgtcttcatt ctgactcctg
    49081 actcctgact cctggctcct gttttatccc tttcgtcctg aagcgcggcc ctttcgtccc
    49141 acgccgcttc acagggcggc gcgggggcgt ttatactcac gcgtttcgac ggggtgggcg
    49201 gcgcgcgact gtgggtgctt tctatggatt tgccgactga gaccgagaag aagccggtgt
    49261 ggcagaggcg ctggttcctg tgggcgctga ttttcggcgt ctggacgttc ctcgccttct
    49321 tcttcacggg gcaggtctat ttctcccggc gcatcatgga gcgcccgata ccgtggtcgg
    49381 aggtcgctcg ggaacagttc atctacccgt acatgtgggc gctggcgacg ccgctcatcc
    49441 tctggctcgc cggccgctat cccgtcgagc ggccccgtct caagcagcac ctgctcatcc
    49501 acctcctctt tagtacggcg ctcaccgtcc tcatcatgag cgccttccag ttcgtctatt
    49561 atgtgctcta cctcatgccc cagggcaaga ccttctcccc ggtgagcatg ctgcgcgcca
    49621 ccgtcttcaa cgccagcgaa aactacggcc tctacggcct catcgtcttg ctgcaccacg
    49681 tttatcgcta ctacaaccgc ttccggcagg gtgagctgcg cgcttcgcaa ctcgaagcgc
    49741 gtctgtcgca ggcgcagctt caggccctca agatgcagct ccacccacac ttcctcttca
    49801 acacgctcca ctccatctcc tcgctcgtcc acaaggaccc ggaggcggcc gacaagatgc
    49861 tcgcgcgcct cggcgacttc ctgcggatga cgctggagaa ctccggcgcg caggaggtga
    49921 agctcaaaca ggagcttgag ttcctccgct gctatctgga gatcgagcgc atacgcttcc
    49981 gcgaccgcct caccaccgag atgaacatcg acccgaagtc gttagacaca cgcgtcccca
    50041 acctcatcct ccagcccatc gtcgaaaacg ccatccgcca cggcgtagcg ccccgctccg
    50101 caccgggccg catcgagatt gagtcgaaac agcacaacgg caccctgcgt atagaggtca
    50161 gggacaacgg gccggggctg ttaccgaatc aaagggccga cggcctgtta cacaaaggtc
    50221 tcgggctcgc caacacgaag gcacgcctcg aacagctcta cggcgcggcg caccgtctcg
    50281 agttggccga aaatcccggt ggcggcctgc tggtggcact ggaaattccg acccgccgcg
    50341 agccggccct ctaaagtcct aaacgcctga taagaaaagc attcggcccc gcgcgagcgg
    50401 ctacttgccg acccggcccg ccacaaaacg ggcgtgggcg gtgttctatt tttaagaacg
    50461 ggtgcgagaa gtttcggcaa cctgcgggcg gtcacgcgcg tgtaaggggt ctagagaggg
    50521 caggtaaagg atggcttcgg acgccccctc gcgaatccgg gtgctcgtcg ttgacgacga
    50581 gcctctggcg cgagaaaaaa ttcgcgagat ggtcaagcgc gacgcggaga tcgagatcgt
    50641 cggcgagtgt tcgagcggcg ccgaggctgt ctcggccgtg cgcgagctgc gccccgacct
    50701 gttgctgctc gacgtgcaga tgcccgaact cggcggcttt gaagttcttc aggcgctcga
    50761 cggcgagcac ccgcccgccg tcatcttcgt cacggcctac gaccagtacg ccgtgcgcgc
    50821 cttcgaggta cacgcgctcg attaccttct gaagcccttc gaccgtgagc gtttcgaggc
    50881 cgcgatgggg cacgccaagg cgcacctgcg cggacagggc cgcggcggcg gcgactcaaa
    50941 cggcgccggc ggcccgttcg accggaggat tctcgacctg ctcgaagagt tgaaggccga
    51001 gaagcgttac gccgagcgac tcgtcgtcaa ggacgcgggc cgcgtcttct ttctggagac
    51061 ggccgagatc gaatgggtcg aggccgaggg caactacatc aacgtccaca cgcagaagaa
    51121 gtcgcacctg ctgcgcgaga ccatcagcgg actcgaagcg cagctcgacc ctcggaagtt
    51181 cgtccgcatc catcgctcgg ccatcgtcaa catcggcagc gtccgcgagc ttcagccgtg
    51241 gacgcacggc gagtaccacg tcatcctgcg taacggcacg agcctgacct tgagccgcaa
    51301 ctaccgggag aaccttcgca acgtcctcgg caacgggctg tgacgtgcgc cgaacggcgc
    51361 gcggcggggc gcgtcggagt cgaactgtaa attgattgta aagcgcgccg cggccgcaca
    51421 cggcttcgag tccgctccgg cacgcgcctt tgccctttca tcacaaaata cttccgcgca
    51481 tgcgtcctga tcaattaact tcgacgcgtg ccagcccgcc gccgttcccg gcgaagacca
    51541 tgtgtgacga gcatgcccgc cgcgtcgcac gctcagcccc gtggtcgcgc cagaagggtc
    51601 tcggctgtca tcgaatgtta agcgtccgaa tcgggaactc aattagtcga aggagggaat
    51661 caattgtgcc agggaggtgt gccaatgaga tttcctcttg ctccgaatgc gttcgcgctg
    51721 atgctctgcg cccccatctg ggtggcggcc ttcgagacgc cgccctgcgt gccccccgac
    51781 gcctgcgcca cgttcgccga gagcatggcg ataggtgcga ccgccggagg gcgagacgac
    51841 ttggcggggc cgccgatgcc gactgaagca gccccggtga aaatcatgct cgaagacgcg
    51901 cagcgggctg tactccttga agaagcctac aaggatgccc gcgacgtgct ggcccgagat
    51961 gatgagtgca gcgacttctt cggcggacgt gacggcgcac aacacgtcct tggccggctc
    52021 gtcggtcagt tgcgagacga cccggcgctc gaaccaaaca taggcatcgt gatgaagggt
    52081 gactatacga acatcatcaa cttacaaagc ggcttgagct atcgcctgtt caccgaggcg
    52141 attgtcaaca gcagaggccc cttcttccgt aagaacgacg gccggcgcgt gttcccggac
    52201 tgcggcagct tcgcgccgaa cacgcggggc gtgcgcgccg cgatgctctt gcacgaactg
    52261 ggacacctga ttcaaggcgc caatggccgc tggctgctgc cgaacgacgg gaacaacccg
    52321 atgttagccg agcgcaacag caaccgcatc gagaagaagt gtggccggca gatcaaagcg
    52381 ctcgacgcca aaccggcccc gagcgatctg ggaacggcga aacgcgctcg cagttgaagg
    52441 gaaagttaaa cgaggcttca acgccgcgcg tttaaagctg cgggcgggcc tgggaaacaa
    52501 tagggaggtt tacgagatga ttaacgtcgc actactggat cacgaagtca tccgcgcgcg
    52561 cgagggcgtg agagtggaag gctccgcggg cgagatcgac ctcgcgcgca aggtcgagtc
    52621 gctacgcgag gtggccctgt cgctcctcaa gcaggtggag cagctcgaag agtcgctctc
    52681 ggccagaggc acggcccgca gcaccgacct ccacgccgag gtgcaacgct ttgagaccga
    52741 aatcatccgc gacgcgctga agaagacggg cggccaccag cgccgcgccg cgcgcatgct
    52801 cggcgtcaag gtttcgaccc tcaacgccaa gatcaggcgc tacggcatcc gcccggagga
    52861 cttgaacgac gcgggcggcc tccgcctcgt cggcgaagga gcctgacgcc gccacagagg
    52921 acggccgccg cgcgctcttt ccgcggccga agttcttgac acggtttcgg caggctgcct
    52981 atattgatgc ccgcggcggt cgtcggttcg acccgaagag tagttcacaa gtttacagac
    53041 gcgatgcacg tcacacgttt ttattacttc gcctttacgt tccgcttctc cacaggggag
    53101 gggcggctcg ggcgaagcac gcgcgcgcgt cgaaggtttg agggctaagg cccggacgcc
    53161 gcgaaagagt ttccgagagc cggccgcgac ctccccgaga gaggtcgcgg ccggctcttt
    53221 ttgttttcaa gttgagtgcg tcgccgggac accggcggcg cggccgagtc ggaaggagac
    53281 acacgagatg aaagatggga attggcagac ggaggtcgag gtcatcgacg gcgaggtgct
    53341 gcggctgttg gagcgccggt cgcagatcgt cgcgctggcc gcgtgcgccg gcgactacgc
    53401 gcgggcggcg cggcaggtcg atacgagcgc cggcggcgaa gcgcgcccgc gacgctcacg
    53461 ggcggcgtcg ctctacgcct cacagccgca cccggaggcg cacgtcgccg ccggtgattt
    53521 gccgtgagga gctattagcc atttgctgtt agctgttagc taaatgctgt ccgttgtccg
    53581 tggtcagtca tccgttgctc gcggcccgcg gcttgagccg tccgacgcga gcgggaagaa
    53641 gcgtcaacgg aaagcgttta gctaacagct aatggctaac agctaacagc ttcttgcgtt
    53701 agaatctgtg tgtgatgagg ctcagtcgtt tctcggtttt cgtgtggggc gtggtggcct
    53761 tcaacctcgg cgtgatcgtg tggggtgctt acgtgcgcgc gtcgctgtcg ggcgacgggt
    53821 gcgggagcag ttggccactg tgcaacggcg aggtggtgcc gctcggcgcg ggcgcgaaga
    53881 cgctcgtgga gttcacgcac cgcgcgacga gcggcgtggc gctcctgctc gtcgtcgcgc
    53941 tcgtcgcgtg ggcgctgcgc gccctgccga agcgacaccc ggcgcgagcc ggcgccgtca
    54001 tgtcgctcgt cttcatcgtc gtcgaggcat tgatcggggc gctgctggta cgctatcagt
    54061 tcgtggccgg caacccctcg gcctcgcgcg ccgtctggat ggccgtccat ctcgtgaaca
    54121 cctttctact gctggcgtcg ctcgcgctga cggcgtggtg ggcttccggc ggcacggccg
    54181 tgcgtctcag gcggcagggg acgctcgggc tggtcgtcta cagcgcgctc gccgggacac
    54241 tggtgctggc ggtgagcggg gcggtggccg cactcggcga cacgctcttt cccgccggct
    54301 cgctggccga agggttgaga caagacctgt cgccggcggc gcacgcgctc gtccgcctgc
    54361 gactgttgca cccgctgctg gcattggtcg tcggcggcct cgtcctcttc gccgccagct
    54421 acgccaaccg cgtgcgcgcc ggcttgtggg tgggccgact ctcaggcgcg gcggccgcgc
    54481 tcgtgctgtt gcaactcctc gtcggcgcct tcaacgtggc gctgctcgcg cccgtcggcc
    54541 ttcagctcac acacctcctg ctcgccgacc tcatgtggat cgcgctcgtg ctgctcgggg
    54601 ctgcggccct gtcgcacgag gcgcacgcgg ccgccgcccc cggcttcgag ccgtcgggcg
    54661 tgccctcggc ggccgggtct tcctgaatca tccgcgccgc gacaaaatcg agagacaagc
    54721 gtgattcctt ccgcgccgcg cccgcgcatc ataagggcac gatgcacggg gataatcact
    54781 tggggataca agacgacgcg cggacgacgc cgaacctcgg cctcgtctgc atcaccacca
    54841 cggacgccgt gcgttaccgc acgacgacgc gcaagcggct cttgcagttt gagaccgccg
    54901 agcagaagcg acgcttgcgc gaactctacg ccgccaacct ctcgcgcctc gacggcgcgc
    54961 tcgacttctg cctgtcgcaa gacttgcgcc tctatcgcat gacctcgggg ctgttcccgt
    55021 tcgccgacga cgaggcgggc gccgacgtgc tcgaagagtt tcgcagtgag gcggcgcgcg
    55081 tcgggcgtcg cgccacgcaa cggggcatcc gcctcgtgct ccaccccgac cagttcgtcg
    55141 tcctcagcag cgactcgccg cacgtcgtcg ccaacagcat caagattctg gagacgcacg
    55201 ccctcgtcat ggacctcctc gaacagccgc gctccgcgtg ggcgctcgtt gagattcacg
    55261 gcgggaagtc gggccgcgcc gcgcagctcg tgagcgtggt gcgcgacctg cccgacgccg
    55321 tccgctcgcg cctcgccttc gagaacgacg agtactcgta cagcgccgcg gagattctcg
    55381 aagtctgcca cgccgccggc gtgccgatgg tcttcgacgc gcaccaccac gtcgtcagcg
    55441 agcggctgga atcttacgac cacccgagcg tgggcgagat gctgcgctcc gcgcgcgcga
    55501 cgtgggccgt gcccgagtgg cagctcgtcc acatctccaa cgggcgcgag tccttcaacg
    55561 accggcacca cagcgacctc atccacgaca tgcccgaggc gtaccgcgat gcgccgtgga
    55621 tcgaggtcga ggcgaaacac aaggagttgg cgatagaaaa actgcgcgcc gggtggctgg
    55681 cgcagggcgc agcggcataa ttcccaaatc aactttcagt tgaacgaagt gacgatgctc
    55741 cgcgagtcgg ctaaaaaagg gcgcaagctc agtcgctcct tctacgctcg cgacgacacg
    55801 ctgctcgttg cgcgcgagct tctcggcaag cgtctggtcg tgccggcggc ggacggcgcg
    55861 cgcgtctcgg gcctcatcgt cgagaccgaa gcctacatgg ggccggagga caaagcctcg
    55921 cacgccttcg ggggccgccg caccgcgcgc accgagatga tgtacgcgcg gggcggcgcc
    55981 gtctacgtct atctcatcta tggcctgcac caccagttca acgtcgtgac cggaacggaa
    56041 ggcattccgc acgccatact cgtgcgcgcg ctcgaacccg tcgagggcgt cgatgtgatg
    56101 cgcgcgcggc gcggcctcga agacgtgcgg aggctcgcga gcgggccggg caagctctgt
    56161 caggcgcttg ccctcgaccg cggctataac ggcgaagacc tgacgcgaac gcgcgtgtgg
    56221 cttgaagacg cgggacgccc cgttagccct cgcgaaatcg cgcgcggccc gcgcgtcggc
    56281 atcgactacg cgcaggagta catcgccaaa ccgtggcgct tctgggtcaa agacaacccc
    56341 ttcgtcagca aaaaggctta acgtcgtcat cgcgtccttt gcgccttcat tgagaaacag
    56401 taccgcgagc ggcagcctcg cgggtgtgtc tacgatgagc ggcccgctta gcgcatgcga
    56461 gacccgcgag gcttccgctc gcggtactgt gcttctggca tatgaagact cacgcgaaag
    56521 gcgcaaagat aaggcgcggt tgaaacgtgg ggagggggtt ggtcgcttgt gtgccggcgg
    56581 cggattgctg ttacattaga cagcgacttc acacggacgg gaaaaccgaa cttgcagata
    56641 actttcctcg gaaccagcgc cggcgtgccg acgcgctcgc gcaacgtctc ctgcgtcgcg
    56701 cttcgccttg agcagcgcgg cgaggtgtgg ctcttcgact gcggcgaggg cacgcagcac
    56761 cagttcctgc gctgcaacga actcaagatc agccagatca cgcgcgtctt catcacgcac
    56821 atgcacggcg accacgtctt cggcctcatg ggtcttctgg ccacctgcgg catggccggg
    56881 cacgcccgac gcatcgatgt ctacggcccg ccgggactcg ccgagtacgt ccgcgagtca
    56941 acacgccgct cgcagacgcg cttctcctac ccggcggaga tacacacaat cgagggggcc
    57001 ggccgtatct atgaagacga cgagtactcg gtcgtctgcg acgcgctcgt ccaccgcgcg
    57061 ccgaccttcg gttaccgcgt gacggagaaa gaccgaccgg gccgcttcga cgtggagcac
    57121 gcccgcgcgc tcggcatccc cgaggggccg ctctacggcc gcctcaagcg cggcgaaagc
    57181 gtggagctgc cggacgggcg catcgtcaac ggccgcgatc tcaccggccc cacggagccg
    57241 gggcgcagcg tcgcctattg caccgacacg gtctactgtc aggcgtcggt cgaactcgcg
    57301 cgcgacgccg acgtgctcat ccacgaggcg accttcgcgc gcgacgagat agagcttgcg
    57361 cgccagagca tgcactcgac gaccgagacg gcggcccgcg tcgcgcttga agcgcaggcg
    57421 cgtcgcctcg tcatcacgca catcagcccg cgctacgccg aggtcaaccc cgtcaccccg
    57481 caagacctgc tcgaagaggc ccgcgccgtt ttccctgata cctatctcgc ccgcgacttc
    57541 ctcaccctcg aagtcccgag acgtgtgtga ggccgcggcc cgcaccgcac ttcactcgtg
    57601 gcgcagcgcg ataacaggat cgacgcgcgc ggccctgcgc gccggcaggt acatggcgaa
    57661 gagcgccacc gtcgccagca gcagcgagac gccgccgaac gccaaagggt cgagtggact
    57721 taacccgtag aggaagccga cgagcacctt cgagagcgcg gcggccccga tgagtcccgc
    57781 gacgacgccc gccagaacga cgcgcatccc cccgccgatg acgagccgca acacgtcgcg
    57841 ccgtcgcgcg cccagcgcca tgcggacgcc tatctcgtgc gtgcgttgcg cgaccgagta
    57901 agagatcacg ccgtacagtc cgatggacgc aagcaccagc cccatcgccc cgagcacgct
    57961 gcacgccaac gtgagcacgc gcgccggcag caactgctgc gcgaccgttt cctccagaag
    58021 cgtcacgttg ggatagattc cccggtcgaa ccgctcgacc tcgtcgcgca ccgcgcccag
    58081 cacggagcgc ggctcgcccc gcgtgcgcgc cagcagccac acctgcacct cgttcgtcgg
    58141 ccgcagcggc aggtagacca gagcatcatc gacttcgctg atgcgaacgc tgcgcacgtc
    58201 cttcgccacg ccgattacct cgcacgaggg cgagtaaggc tccggctcga actgccgccc
    58261 cgcagcgtcg cgcgccggcg cgacccccag acgcacgcgc cggcccagcg gctcctcgcc
    58321 gggcacgaac agacgccgcg ccgtcgcctc gctgagaatg gcgacgcgcg gcgccgtctc
    58381 gcgcgtctcc tgctccgtga agcctcggcc gcggaggacg gcgatgcccg tcgtctcgaa
    58441 gtagttcggc gcgaccatcg tgtagtaggc ccggagttgc cggccgccgc cttgcgcccc
    58501 cggctcagcg acggccgcga ccgccgtctt gcgcgcgccg gccgtccgcg tgctcagtgt
    58561 gacggactcc acacccggca acgcttgcag acgctccgag agttggcgat gcatctccgc
    58621 ggtgcgcgcg gcgtcgtggc cgagcgagag caggttgggt tcgacgacga ggaagcgcgc
    58681 ggtctcgtag ccggcgtcga gcgactggcc cttgcgcacg cccctgatga gcagcccggc
    58741 cccgacgagg agcacgagac agaccgccac ctgcacgccg acgagcacat cgcgcaggcc
    58801 gagccggaag attccgcgcc ggcggacgcg cgccgcgccg ccgtccgcct gtttgagcgc
    58861 cgagacgagg tcaggccgcg acgcctgaag ggccggggcg aggccgaacg tcacaccggc
    58921 cacgagcgtc agtagcagcg tgtaagcgaa gacgcgcacg tccgggctta agtcaaacgc
    58981 ggtgttgacc acgcccatct ccggcggcag cccggcgagc acgaccttga gcaccacgtc
    59041 gagcgtccac acggcgagca gtagccccaa cgctccgccc gcgaacgaaa gcatcaggct
    59101 ctccgtcacc aactgccgca ccagacggcc gcgcgtcgcc cccagcgcga ggcggacggc
    59161 aatctccctg cgtctcgcgg acgcgcgcgc gagcaggagg ttggcgacgt tggcgcaggc
    59221 gacgagcagc accaatagat cagccgcgag gacgaggaag aacatcgtgc gcaggaagcc
    59281 gtcgagccgc gcgaacgacg acgccggctc gaccgccgca ccgacgcgct cattcgtctc
    59341 cgggaacgaa cgcgcgagtt gcccggcgag cacatcgatc tcggcttgcg cctgctccgt
    59401 cgtcacgccg ggtttgagtc gcccgaccga gcggaagaag gtcgcgccgc gatcccggct
    59461 gtcgaagatg gggagcacgc tgttctgcat cgcgacgggc acccacacat cggggacgag
    59521 cggcgtcacg ccatagaagt cggctgcggc cacgccgacg acgttcaact gccggccgtt
    59581 gatcgcaaac gtcttcccca gaacgcgggt gtcggcgccg aagcgtcgct gccagtattt
    59641 gtggctgagg acggcgacgg ggtgcgtccc ctgtgtcgtg tcctcgtcgg acgtgaaggt
    59701 gcgacccagt tgcgtcgcgc cgcctagtag ggagaaatag ttgcccgaga cgaaggcgat
    59761 ctgcgccacc tcggcccgcg cgccggcgtc ggccgccccc gcttcggaag gcgactcgct
    59821 catcgtcagc ggggcggggc cggcgtagac gagcatcccg gcgaagacct gattgtgttc
    59881 gtcgaagtat gcgtggtcgg ggtaggaagt ggtctcggcg cgcgtgccgt cggggttgac
    59941 gcgctcgacg cggacgagcg cgccggggtc gcggacggga aggggacgcc acgcgaaggc
    60001 gttgcagagc gtgaagacgg tggtgttgac gccgatgccg agggcgaggg tgaagacggc
    60061 cgcggccgcg aagccggggt tcttccgcaa cacgcgcgcg ccgtagcgca ggtcgtgcca
    60121 gacggtctcc agccagccgc cgccgcgcac ctcgtagccg cgctcacgca cacgcgtgcg
    60181 gttgccgaag cgacgcaaag cctctcggcg cgcctcctcc ggcgacatcc cacgccgcac
    60241 gttctcctcc gcgcgcatgt cgatgtggaa gcgcatctcc tcgtcgatct cgcgcccgat
    60301 ctcgtcggct cgccagaggc cgcgcgccct ccgcacaaaa gacttcatcc agcccatccc
    60361 ttcgacctcc ctcaagccgt ctgcaagacc ttgccgaccg ccagcgcgaa ccggcgccaa
    60421 ctctcctcct cgacgtgtag ctgccggcgc ccggcggccg tgagcttgta gaagcgcgcc
    60481 ctccggttgt tctccgacac accccactcc gaggtgatcc actcgcgctc ttcgagccgg
    60541 tagagggccg ggtagagcga cccctcctcg acgcgcaaca tctcctcggc catctgcgcg
    60601 atgcgctgcc cgacgccgta gccgtgcatc gccccgtgcg agagcgcctt caggatgagc
    60661 agatcgagcg tgccctggag caaatccgcc tgtttatctt cacgccgctt ggtcatcgat
    60721 atctccttcc ctagacaagg taggggagaa gtctaaatca tctttcccta ccttgtctag
    60781 gggagaggcc gtctgcgccc atgaagaagt ggaagacgtg tcgcgccggg aacatctcgc
    60841 cacgaaactg cgggcggcgc gtgatgactg tgccaattgc cctgtgtaca acctctgccg
    60901 gctgtgatac gatgtgccac gattgcaaca accggcgcgc ggtgtgtcgg cggcacttca
    60961 agagcttacg agagaagagg acaagatcgt gtcattcaat aaagtgatcg tggtaggaaa
    61021 cctgggcaaa gacccggaga tgcgcttcac gccgcagggc acgccggttt gcaccttcac
    61081 cgtcgccacg aacgagcggc gcaaggacaa ggccggcgag cagcaggacg tgaccacgtg
    61141 gttccgcgtc acggtctggg gcaagcaggc cgagaccgtg tcgaagtacc tgacgaaggg
    61201 ccgcagcgtc tacgtcgagg gccggatgca cgtcgaggaa tggacggaca aggacgggaa
    61261 gctgcgtttc actctggagc tgaacgcgag cgacgtgcgc ttcatcgacg gcgcgcaggg
    61321 cgcaccgtcc gaaggcatcc ccgtccggca gacggggcag gtgcgtgagt cggccgcgcg
    61381 cgcgggcgtc ggcggcggcg gtcgcgcgcc ctcgtccgcc gcggacatcg acgacgactc
    61441 gatcccgttc taacgccgtt gcggcgggcg gcgcagacta aaaacctaac gacgcggaca
    61501 aacgcgaggt gattgaattc ccgtgcggcg gggcgttgag acgcgcgctc atgcgttcgt
    61561 ctcgccggcc cggctgttgt atcttcgacg cgtgaaggat tcgacgacgc tcgtcttaag
    61621 cttcgaggcc gggacgctcc tgctcgaagg ggttggcccc acagagaaag cgcccacggc
    61681 cttccgctgg gacgagcgcg tcgggcgctg gcgcgcgccg gccagctcct accgtcaggt
    61741 cgtcagcgag ttgacgcgcg gccgcgtcgc cttccacgac cgcgcgcgcg gctatcatca
    61801 gttcgacttt cccaccaagt tcctcgtcga gccacggccc taccagcacg aggcgatccg
    61861 cgagtggaag gccgcgctag ggtgcggcgt cgtcatcctg ccgacgggcg cgggcaagag
    61921 tctcgtcgcg cagatggcca tcgagcaggt gaagcgttcg acgctcgtcg tcgtgcccac
    61981 gctcgatttg atgaaccagt ggtacgacct cctgctctcc tgcttcgagg ccgaggtggg
    62041 actcatcggc ggcggcttct acgaagtggg cgcgctcacc gtgacgacct acgcctccgc
    62101 cttccgcttc atggagcgac tcgggaacgc gttcggcctc gtcatcttcg acgagtgcca
    62161 ccacctcccc tccggctccc accgctacgc cgccgacctg tgcatcgcgc cctaccgtct
    62221 gggcctcacg gccacgcccg agcgcgccga cggcggcgac caacttctcg aacaactcat
    62281 cggccccacc gtctatcgcc gcgaggcgcg cgagttcgcc ggcgaatatc tttcggacta
    62341 cacggtcgag cacgtcagcg tgcgcctcac gacggaagag cgcgcccgtt acgagagcga
    62401 gcgcgaaatc ttccgtcgct tcctgagcga aaagggaatc ggcctcggct ctttgcgcgg
    62461 gtggcagatg ttcgtcgcgg cgagcgcgcg cacgagtgag gggcggcgcg caatgatggc
    62521 ctaccgcgag tcgaagcgca tcgcgctcgg cacagacgcg aagctacgcg tgctgggcga
    62581 gttgctgctg cggcaccggc gcgagaaggt gctcgtcttc accgccgaga acgagatggt
    62641 ttatcgcatc tcgcgccgtt tcctgatacc cgccatcaca cacgagacca acgtcaagga
    62701 gcggcgcgca tggctcgaag ccttcaacca cggcgacgtg ctcgcgctcg tcacctcgaa
    62761 ggtcttgaac gaaggcgtca acatccccga cgcgtccgtg gccgtcgtgc tctccggctc
    62821 cggctcctcg cgcgagcacg tccagcgcct cggccgcatc ctccgcaagc tgcccggcaa
    62881 ggaggccacg ctctacgagg tcgtcaccga agacacgacc gaggagcaga tcagccgccg
    62941 gcggggcggc gaacagttcg acgcgaagcg acaacgcgcg ccgaccaccg ccgagatacc
    63001 cttctaaaga ggactggaat gctgacggcc gacctcgcga tgagctggca acgtggcgaa
    63061 caggttcggc cgcgtcagct cgccacgaaa gacccgcgcc acctgcaaac tgccgccgac
    63121 ctcgtcgcca tcttcgaggc gcacgcgggg cgcgagcggc acgagctgga cgaagctctg
    63181 ggcgagtacg tcggcacggg gacggactac cgcgtcctgc gcggcctgat aaaacttctc
    63241 actgaccgtt gtcgcttcga gacggacgtg tccgtcgagc cttccgagat tcgccgcgcg
    63301 ctcttcctca aggcgcgcga gcgccacccc gtcgcgggcg cggacggcgc ggcgcgcttg
    63361 gatgtcgccg ccgaagtcgc gtcctccctc gggtgcgacg ccgagaccgt gctcaggagt
    63421 atgtacgccg acctaccggc cgagcagcgc ctgctggagt tcgaccccac ggggccgggc
    63481 gaactcatcg acctctacaa cctcgcgcag gcacaggcgc tcctctaccg ctcggtcgag
    63541 atgcgcctgt gggtcgagcc gcaggggcct gacggctacc gcgagttgtt cggcgccatc
    63601 aaggcgtacc ggctgattca caccgtccga ggcgacgcgg ccacgggcta cgagataact
    63661 ttgagcgggc cggtctcgat gttccaccgc tcgcagaagt acggcgtgca gatggccgtc
    63721 ttcctgcccg cgctgctact ctgccgcggc tggcgcatgc gcgccgagat caacactaga
    63781 cacggccacg cctacttcga actcgacagc cggcagacct ctctgcgctc acactatctg
    63841 gaagtgaccc cttaccagaa ccccgcggcg gagaagttcg cggagagctg ggagcgcgcc
    63901 ggctgcgcct cgctgctgga gccgaaccga gaggtcatcg gccacggcga taccgccttc
    63961 atccccgact tctctatcac gcacccctcg ggccgccagt tctatcttga attcctcggc
    64021 ttctggacgc ccgagcacct gcgcgcgcgc ctcgcggagt ttgagcgaca ccgcctgcgt
    64081 aattatctcc tcgccgtgtg ggaagacctg cgcgggacgc gcgagccgct cacgaatgtc
    64141 ccgccgaacg tcgtcgtctt caaaaagaat ctcgacccct caatcgtcga actcgccatc
    64201 agtaaactcg tcgcggccga ggcgtaatcc gtcacacttc ggccggcgtg cggcgccggt
    64261 cccgtggtcg cgaaatctgt tgcaacgcga ccgggagttg cgatatagat gccgccgcgc
    64321 ggggcgcggg gactgatccg ccgggacacg ttacccgccc cctccggttg aaaacttcgg
    64381 acggccagac actcgccacc ctgtctgtcg ccactcacgg gcgcgcgcgt cacaccgcac
    64441 atcccatcct gcctacgcag gggccgcaaa ggatcggctg ctatggccgt aatgatcgac
    64501 ggaccatcca cacgacaaca gtccgaggcc gcgccggagc aggcgtcggt gaccgcaccg
    64561 gagtctctgc gaggcccctg catcacgaag ctggtcagtg acaagcccgt cgagcaggtc
    64621 gggtcggacg atgaaggcgc gcacaagcgt ctggcgtctg cgcttgccga cctcatgcgc
    64681 ggcgaggaga agggtggcgt ctccgtcgga ctcgaaggcg actggggctc cgggaagacg
    64741 agcgtcctca tgctctttaa gcaggagttg cgagacgccg gggaactcat cgaaccgtcc
    64801 cccgaggacg agggacaaga gggggagaag gcggcggggg cggccttgcc tgcccccggg
    64861 agcggcgcca aagatttcgc cgtcatcacc ttcgacgcgt gggcgcacga gaaagaccct
    64921 ctgcggcgca ccttcctcga aaccatcatc ccgcacctcc tgagcattgg ctgggtgcgt
    64981 aagagcgaca gttggcgctg gaagaggcgt ctacagatca tcgcccaacg acttgaaagc
    65041 aagactactg acagcagccc gcggcttgag ccggccggcg tcgctgctgt gttgtcgctg
    65101 ttcctcgtcc ctattggcca cgacctgttc tccgaggccc tcaagaccac gcagttcgtc
    65161 tgggaccctg accggcccat cgcgtggagg ctcctcgtcg gcctcttctt gatggtgctg
    65221 ccgctcctgg tggtggcctc cgcttacgtc cgctggtggc tctcgggaag cggggagcgc
    65281 ccgtcgcttt gggcgctgct cttcaacaag ggcatcacct ccgagcgcac gcgaaccttt
    65341 aagacggcca acccgacctc catcgagttc gagcacgagt tcacgcagtt gatgaaggag
    65401 acgctcgaag ggaaagtggt gttggacgag gacgaggaaa aggagcagga ggaggcgaag
    65461 agaaaaaggg ataacacgaa gggcgctgtg gtcgaagccg atgtcgatta caagcgccgc
    65521 cggctcgtcc tcgtcttcga taatctcgac cgcgtggaca ccgccgacgc gctcaccatc
    65581 tggtcaacgc tccagacctt tctgggcagc ccgaacgcag cgcagagcgc ctggttcgac
    65641 cggctctgga ttctcgttct ctacgacccc cgcggactgc gtctgctctg ggacaagata
    65701 gaggagaagc ggctggggca accgggtgaa cgggccgcgc ctgctgaccc gacatccgcg
    65761 gccggcgtcg ggggccaagc cgccaagccg gcgccgggcg gttccgctga ggcgtccaaa
    65821 gccgggccgg gtggcgcgcc cggagggcag gacgcgaagc cagcgacccc ggcggccgcc
    65881 gtcgctggcg cgcccgcagg acaggcgccg ggggccgtcg aaggctgggc agcgccggtg
    65941 gcttccgcca tcacccgcgc ggcttcaacc tcgttcatgg acaagagctt tcagattcga
    66001 ttcgaggtgc cgccccccgt gctctcggat tggcggcagc gcctccgcgg gttcctcgat
    66061 gacgccatcc cgcgtcacgc cggggagttt gattcgattc tcggcgtcta caggctctac
    66121 ctgagcgaaa cggagcagta cccgacctac agggaattga agctcttcgc caaccagatc
    66181 ggctcgctcc accgccagtg ggctaaatac tgcggcccgg cccccacagg gcaggagaag
    66241 aattgggagg aaagatttcc actgagcaac gtcgcctact acgtcctgct ccggcgtcgt
    66301 ggggttgatg tcgtcaaggg gttgctgggg gggcgtctcc ccgaagacag ttacaagggt
    66361 ctgctcggcc ctgtcgagca ggcgcgcgac acgctggcgg cgctggcctt caacgtcagg
    66421 cgccggacgg cgcggcagat cgtcctcggc aagccaatcg gcgacgcgct cgaaaggggc
    66481 gcggaggggg ccgcgaaact ggcggagctc aaggagagcc atgacggctt ctggggggcg
    66541 ctcaaggacg tgctcgacgc ccgtgggggc gaaagcgtcg cgaccccgga ggacgccgac
    66601 cgtctcaacc gggcgattca gtgcatccac aggagcggga tgttcgacag ccccgagccg
    66661 gacaacgacg acctcggcgg cgccgtgaaa gtgttgtgca tgagggccgc ggccgtcgcg
    66721 gagtggccct cgttcgacag cccgacggcg gagggactgg ccgccctctg ccgctgggcg
    66781 cggcacccga aggtctggca cgacggctgg cacgacaggg tggaattcga gtcgaacctc
    66841 ctcaagaacg tatcgcggag ccttcagccg gacgaaaaag tgaggcccgt cgagaaggcc
    66901 ggccagtggc tggagaacgt ctccgtggtg gccgcggaaa tcttcacgga ggtcgatgac
    66961 gcgccggaga agcagcagca gcacgcggcg atcctcgacc agattgccgc gcaacttctt
    67021 cccgaagacg ttgaggtgtt caccgatgaa gggaggatgc tcaaagcaaa gtatcaggaa
    67081 cacccgccgg cgcgactaag tctgctgctg gaagcgttga tgatactgcg taacttaaac
    67141 cctggagggt cggagatgca gcaagtcatc aaccaactgg tgaacattga ctatagcctg
    67201 cttcactacc tggaagacgc ttactccgcg cccgaaggtc gcgagcgcac acaggcgctc
    67261 gcacgaagca tgttcctcca cctcaacgaa gacccttcag ccagccagcg gtggggcatc
    67321 tccaccgagt tagaaggctc cgcgaggctc cgcgaactcc tgagcgcctc cgaagaagac
    67381 gaagcctcgg ccagccccaa cgcgccgggc gtgaatctcg ccgaggaatt cgtctacgtg
    67441 ttgaacgacc acggccgcct gccgctcctt tttgaactgc tggataaagc agcggagcga
    67501 aaagtggtcg agccgttcgt cgcgcgttgc ctgagggtgg tggcggacga ccggaacttc
    67561 ggcaagatac ttctcacgcg ggagacgctg gtcgagaggt ggcccgacat ctaccgcctc
    67621 gtcaacggcg acgagcgccc gctggcgggt gcgctgcgcg agcacatcag gagcctgccc
    67681 gccgacgaaa tggcgctcgc ggtcaggatt ttcgatgaca cgcacccgat gaaaaagctc
    67741 taccatgacg ggccgcgtga gtacgtctgg ctgtacgcgg aggtcgtcgg cagcagcgcg
    67801 gaaagcatga aggcgctcga ccagagcaga aagcggtgga ctaatactat ttacggcctc
    67861 gacgccgaac gctggcgccg ggaagtgctg gagtggggcg ccctctacga actcaccttc
    67921 gtccttcagc agaaggggca attcaccgtc gtcccgaagc attacgtcga ggggatgctt
    67981 gaggccgcga aggcggttat cgaaggcgag ccgacggcga cgcgcccgcc ggagcgtgcc
    68041 ccgaccgtcc tgctcggcac gtcggccgac gcctcagtgt ccgacgcact ctaccagtcg
    68101 gccgccgatg caggctgggt tatccccgag gaattcttcg accagtacgg gcgctcgctc
    68161 gccgaatcgg atcagctcag ggaaggtagt ctcgccagaa tcctggagcc gatgctgaag
    68221 aagcgcaacg cccccggact gcgttggttg cacggcgtgt tggccgagaa cagcgagctt
    68281 caggaacgca tcgacttccc agagtttatt agtctcgccc agaaggacat agaagagcac
    68341 aggaacgatc tggcggccgc aatcatgttt gaaatcggca aaatagtggg cgtggttgac
    68401 ccgtccgccg ttgaccaccc gcggttcgaa atagtgctat ttgacgtagg gccacaaccc
    68461 gcgtctgttc tggacctcat caccctcaat acgtctgttg gcagccgggc gaaagaaata
    68521 att
//
LOCUS       DASVES010000155         7652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1370332, whole genome shotgun sequence.
ACCESSION   DASVES010000155 DASVES010000000
VERSION     DASVES010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370332"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..707)
                     /locus_tag="VF240_06310"
     CDS             complement(48..707)
                     /locus_tag="VF240_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX7174879.1"
                     /translation="MKKAITAVVFAAATSLSAFAQQPAAGRTPAAGPATRPAAPATSA
                     APQGGGAAVAANIAIIDITAFGDEKAGIGRLMNAYATLRREFKPRQDELQTLKTRYET
                     LGKEVEQISKVSDDKALNVKVEQMQTLEKEIKRKQEDAQTAVQKREDELTGPVWDDIN
                     NALRAFAGQRGISIILEKSKLAGNGLIFVVNDSVDITRAFIADYNQRNPASAAAAPST
                     R"
     gene            complement(801..3905)
                     /gene="bamA"
                     /locus_tag="VF240_06315"
     CDS             complement(801..3905)
                     /gene="bamA"
                     /locus_tag="VF240_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX7174880.1"
                     /translation="MRTRAPRLLLTAAALVGCLGTYLLGVPPPRTFAQQTTPPQAQPQ
                     KLVEDVQVEGNRRMSDEGVLYYVQTRSGDPYNPEQVQRDLQALLALPFFDKTKTRVSV
                     NEQGPRGGVVIIFTVEELPVIRDLTFPGMKAVSEADVLKGFRENRVGVSKEATFDPVK
                     AKAAERAIKELLAARGYPNATVATEVEEVSRQSVAVAFRVVEGGRVRVVDIDFEGNKV
                     FSDGELRGAMQLVKEAGLISRFKGQDILDTRKLEYDLGYNVTNYMRSKGYLQARTLEP
                     KIEDVGRRRTGFPVLPLPILSSTDDALRIVVPVNEGKVFRVGEVKVEGNSIIDENVIR
                     AILGLKTGDIANGQRLFKALQEDLKRVYGRAGFIQYDYDVEPSFRDNAQSQTEGIADF
                     QITITEGKQFTLRRLEFLGNTFTRDNVLRREVLLSEGDIFDQSLMEFSVLKLNQTGYF
                     DPIDDKKDIDVRTDEERGDVDANIKVVERGRQQISFNGGLAGAAGSFFGLEYSTNNLL
                     GRGESLAFQVAFGNRQRSFLFSFTEPYVKDRPISVGFSVFTESRKFFGEGTFLSSNTG
                     AIDDILGSQITGEIDEENLFTQKSTGASLFASAPLSEFYRRRSPFSRASRIGLSYTIS
                     QSSIADPAVNEGGDPNSFIPQIFDQPNILTSRITPTFVYDTRNGSIDPTRGRQLAIQI
                     PFAGLGGDVRTYQPSVTYTQFIPIRRKNSENPEVFGFRIVAGHVGSFAKTAQVLAAEQ
                     TSLSFINGVPVFERFFLGDEFTIRGYSTRSISPLVPLDFFLSSQGVRLSAVPSGTVED
                     VANLPDTLRQQLIALGTFTGASGTNSLRLSQRDARFLGGDTQLLGNFEYRIPIYSRIL
                     SAALFADVGTSFNLRGNADQTFSSEFLADDPFLLTLGVSSLSSLVGRNNPQLALGPAT
                     GGLILRDNRLVTKEEFSNALRAGPFDPLSGLPFGFQEVFLRGDVQTNTVARLSESLFS
                     KFSDIRSSVGGELRIQLPVVNVPFRLIYAFNPNARDQEVPGIPGLRIRDKKRLFRFSI
                     GRTF"
     gene            complement(4242..6650)
                     /locus_tag="VF240_06320"
     CDS             complement(4242..6650)
                     /locus_tag="VF240_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX7174881.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGAPAIEPEHLLLGLMREDKTL
                     TSRFLARAQASLEAIRKEIEGRAPLREKISTSVELPLAPETKRVLHYAHEESDRLQHR
                     HIGTEHLLLGLLREERSMAAEILYERGLKLHAVREEVARATGVDPRAAQKKETPHLAE
                     FSRDLTDDAANDKLDPLVGREGEIERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIVRGEVPTFLENKRILSLDLSLIVAGTKYRGQFEERLKQIMRELVENPQYIVFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEFRKSIEKDRSLERRFQAVKV
                     PPPTEEEAVQILDGVRERYEAFHQVRYTDDAIEAAVYQSSRYIPDRFLPDKAIDVIDE
                     AGARVKLRARRDPVGGGDAGGVVFGDVFGERGSSSASSGYPSKGVPSSLRLPDDEEEM
                     LLTVEVTRDDIEEVIARWTGIPVVSLKEEETQKLLRIEDELHRRVIAQRPAISALARS
                     IRRSRAGLKNPARPVGSFLFLGPTGVGKTEVARSLAEFLFGSERSMIRFDMSEFMEKH
                     SVSKLIGSPPGYVGHEEGGQLTERIKRSPYSVLLFDEVEKAHPDIFNVLLQVFEDGIL
                     TDALGNTIDFKNAIIIMTSNIGARFIQKKGTMGFQATADASREKLEEMVMSSVRQTFN
                     PEFINRLDEIIVFDQLTDEDLLEVVGLQVELLNRTLSRRGLEVRLTEEARRWVVRKTC
                     ADRSYGARPLRRALQKYVEDPLSEALIQGTLGAPVIEVYLDGEELNHRPAVVEEPGDA
                     LLIH"
     gene            complement(7033..>7652)
                     /locus_tag="VF240_06325"
     CDS             complement(7033..>7652)
                     /locus_tag="VF240_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174882.1"
                     /translation="PGRPRALRPAAGLTPGFHTPKLPKELRPMDKRFLRAALAFACLC
                     GAVSAVSAQQSQGRRTGGGGLDNMSLPSTLERAAGERWPTFAPEGEGFSVAMPGKPVD
                     VAAEKRAGGLADARSRRYVVKADGIVYEVTRTGQIPAALFEVRGFEQDFLASLAGSLP
                     ASARNEWPHMKLELSEQQPTALDGVAGREFDLSSADHRARARFRR"
BASE COUNT         1298 a         2317 c         2678 g         1359 t
ORIGIN      
        1 aaccgcgatt cacgattgac gattgacgat tgacgacttt gcgcgcttta gcgagtggag
       61 ggcgccgcgg cggccgatgc cgggttgcgc tggttatagt cggcgatgaa ggcgcgcgtg
      121 atgtccaccg agtcgttgac gacgaagatg aggccgttgc ccgccagctt gctcttctcc
      181 aggatgatgg agatgccgcg ctgaccggcg aaggcgcgca gggcgttgtt gatgtcgtcc
      241 cagacggggc cggtcagctc gtcctcgcgc ttctggacgg cggtctgcgc gtcctcctgc
      301 ttgcgcttga tctccttttc gagcgtctgc atctgctcga ccttgacgtt gagcgccttg
      361 tcgtcggaga ccttggaaat ctgctcgacc tccttgccga gcgtctcgta gcgcgtcttg
      421 agcgtctgca actcgtcctg acgcggcttg aactcgcgcc ggagggtggc gtaggcgttc
      481 atcaggcgcc cgatgccggc cttctcgtcg ccaaaggccg tgatgtcgat gatggcgatg
      541 ttagcggcga cggcggcgcc cccaccctgc ggcgcggcgc tcgtggcggg tgcggccggg
      601 cgggtcgcgg ggccggcggc cggggttcgc ccggcggcgg gctgctgcgc gaaggccgag
      661 aggctggtcg cggcggcgaa gacgactgcg gtgattgctt tcttcattac tcgtactgct
      721 ccttagcggt tagaagggat ggccggcgct cggtctggga atcgaagtgg cgtccggccc
      781 ggtagtttaa tacgaaacgg ctagaaagtc cgcccgatgc tgaagcggaa gagccgcttc
      841 ttgtcccgaa tacgcaggcc cggaatgccg ggcacctcct gatcgcgcgc gttcgggttg
      901 aaggcgtaga tcagacggaa aggcacgttg acgacaggca gttggatgcg tagctcgcct
      961 ccgacgctgg atcggatgtc gctgaatttt gaaaaaagac tctcggagag ccgggccacc
     1021 gtgttggtct gcacgtcgcc gcgcaggaag acctcctgaa agccgaaggg caggcccgaa
     1081 agcggatcga aggggccggc gcgcagggcg ttggagaact cctccttcgt caccaggcgg
     1141 ttgtcgcgca ggatcaggcc gccggtggcg gggccgaggg cgagctgcgg gttgttgcgc
     1201 ccaaccagac tgctcaggct cgacacgccc agagtcagca ggaacgggtc gtcggccaga
     1261 aactccgaag agaaggtctg gtcggcgttg ccgcgcaggt taaacgaggt gcccacgtcg
     1321 gcgaacaggg ccgccgagag gatgcgggag tagatgggga tgcggtactc gaagttgccg
     1381 agcagttgcg tgtcgccgcc gaggaagcgc gcgtcgcgtt gcgacaggcg caggctgttg
     1441 gtcccggagg cgccggtgaa ggtgccgagc gcgatgagct gctggcgcag cgtgtcgggc
     1501 aggttagcca cgtcctcgac ggtgcccgaa gggacggcgg agagacggac gccctgcgag
     1561 gagaggaaga agtcgagcgg gacgagcggg ctgatcgagc gcgtcgagta gccgcggatg
     1621 gtgaactcgt cgccgaggaa gaaacgctcg aagacgggga cgccgttgat gaaagagagc
     1681 gaagtctgct cggccgcgag cacctgggcc gtcttggcga agctgccgac gtggccggcg
     1741 acgatacgga agccgaacac ctcggggttc tccgagttct tgcggcggat ggggatgaac
     1801 tgcgtgtagg tgaccgacgg ctggtaggtg cgcacgtcgc cgcccaaacc cgcgaacgga
     1861 atctggatgg cgagctggcg gccgcgggtc gggtcgatgg agccgttgcg agtgtcgtag
     1921 acgaaggtcg gcgtgatgcg gctcgtcagg atgttcggct ggtcgaaaat ctgcgggatg
     1981 aaggagttcg gatcgccccc ctcgttcacc gccgggtctg cgatggacga ctgcgagatg
     2041 gtgtaagaca gaccgatgcg cgaggcccgc gagaaggggc tgcgccggcg gtagaactcc
     2101 gagagcgggg ccgaggcgaa caggctcgcg cccgtagact tctgcgtgaa gagattctcc
     2161 tcgtcgatct cgccggtgat ctggctgccg aggatgtcgt cgatggcgcc cgtgttcgac
     2221 gacaggaacg tgccctcgcc gaagaacttc cgcgactcgg tgaagaccga gaagccgacc
     2281 gagatcggac ggtctttcac gtaaggctcg gtgaacgaga agaggaagga acgctggcgg
     2341 ttgccgaagg cgacctgaaa ggccaagctc tcgccgcgcc ccagaaggtt gtttgtcgag
     2401 tattccagac cgaagaagga gccggccgcg ccggccagtc cgccgttaaa ggagatctgc
     2461 tggcgcccgc gctcaaccac cttgatgttg gcgtccacat cgccgcgctc ctcgtcggtg
     2521 cggacatcga tgtccttctt gtcgtcgata gggtcgaagt agcccgtctg gttgagcttg
     2581 agcacggaga actccatcaa gctctggtcg aagatgtcgc cttcgctgag gagcacctcg
     2641 cgccgcagca cgttgtcacg ggtgaaggtg ttgcccaaaa actccagccg gcgcagcgtg
     2701 aactgcttgc cctcggtgat ggtgatctgg aagtcggcga tgccctcggt ctggctctgc
     2761 gcgttgtcgc ggaatgaagg ctccacgtcg tagtcgtact ggatgaagcc ggcgcgtccg
     2821 tagacgcgct tgaggtcttc ctgaagcgcc ttgaagagac gctgtccgtt ggcgatgtcg
     2881 ccggtcttga ggccgaggat ggcgcggatg acgttctcgt cgataatcga gttgccctcg
     2941 accttgacct cgccgacgcg gaagactttg ccctcgttga cggggacgac gatgcgcagg
     3001 gcgtcgtcgg tcgaggagag gatggggagc gggaggacgg ggaagccggt gcggcggcgg
     3061 cccacgtctt cgatcttagg ctcaagcgtg cgggcctgaa gatagccctt cgagcgcatg
     3121 tagttggtga cgttgtagcc gaggtcgtat tcgagcttcc gggtgtcgag gatgtcctgg
     3181 cccttgaagc gcgagatgag gcccgcctcc ttgacgagct gcatcgcccc gcgcagctcg
     3241 ccgtcggaga agaccttgtt gccctcgaag tcgatatcga cgacgcggac gcggccgccc
     3301 tcgacgacgc ggaaggcgac ggccaccgac tgccgcgaga cctcctcgac ctcggtggcg
     3361 acggtcgcgt tcgggtagcc cctggcggcc agcagctcct tgatggcgcg ctcggccgcc
     3421 ttggccttga cggggtcgaa ggtggcctcc ttcgagacgc ccacgcggtt ctcacggaaa
     3481 cccttgagca cgtcggcctc cgagacggcc ttcatgccgg ggaaggtgag gtcgcggatg
     3541 accgggagtt cttcgacggt gaagatgatg acgacgccgc cgcgcggccc ctgctcgttg
     3601 acggagacgc gcgtcttcgt cttgtcgaag aagggcaggg cgagcagggc ctgaaggtcg
     3661 cgctgcacct gctcggggtt gtaggggtcg ccgctgcgcg tctggacgta gtagagcacg
     3721 ccctcgtcgc tcatgcggcg gttgccctcg acctgcacgt cctcgacgag cttctgcggc
     3781 tgggcctgcg ggggcgtggt ctgctgcgcg aaggttcgcg ggggcggcac gccgaggagg
     3841 tatgtgccga ggcagcccac gagggcggcg gccgtcagga ggagccgggg cgcgcgagtt
     3901 cgcataaaat tagctttgtg cctcaagaaa attgccgggt gaattttcag ggagacctac
     3961 agtgtaagaa taactacccg tgtcgagaat aactgccgga cttgggccgc gcgggccggc
     4021 gcttgcgagg gcgacgcctt tccttccgtg actttcgaga cctttgattc ccggccccgc
     4081 ccgccccgtt gccagccttc ccctccagca aagtggcgca ttatacaagg ccgaagttca
     4141 aagttcaaag ttcaaggttc aaagttaagg cttttggcct caatctttga accttgaatc
     4201 cgggcctttg aactttgaac tttgaacttt gaactcgttt ttcagtgaat gagtagcgcg
     4261 tcgcccggct cctcgacgac ggccggccgg tgatttagtt cttcgccgtc cagatagacc
     4321 tcgatgacgg gggcgcccag cgtgccctgg atgagcgcct cggagagcgg gtcttcgaca
     4381 tacttctgga gggcgcgcct gagcggccgg gcgccgtagg agcgatccgc gcaggtctta
     4441 cgcaccaccc agcgccgggc ctcttcggtg agacgcactt cgaggccgcg ccggctgagc
     4501 gtccggttga gcaattcgac ctgaaggccg acgacttcga ggaggtcttc gtccgtgagc
     4561 tggtcgaaga cgatgatctc gtcgaggcgg ttgatgaact cggggttgaa ggtctggcga
     4621 acggacgaca tgaccatctc ttcgagcttt tcgcgcgagg cgtcggccgt ggcctgaaag
     4681 cccatcgtgc ccttcttctg gatgaagcgg gcgccgatgt tggatgtcat gatgatgatg
     4741 gcgttcttga aatcgatggt gttgccgagc gcgtcagtaa ggatgccgtc ctcgaagacc
     4801 tgcaagagca cgttgaagat gtcggggtgc gccttctcga cctcgtcgaa gagcaggacg
     4861 gagtagggcg agcgtttgat gcgctcggtc agttgtccgc cctcctcgtg gccgacgtaa
     4921 cccggagggc tgccgatgag cttcgagacc gagtgtttct ccatgaactc cgacatgtcg
     4981 aagcggatca tcgagcgttc cgacccgaag aggaactcgg ccaaggagcg ggcgacctcg
     5041 gtcttgccga cgcccgtcgg ccctaagaaa aggaacgagc cgaccgggcg cgccgggttc
     5101 ttgaggcccg cgcgcgagcg gcggatggag cgggcgaggg ccgagatggc gggccgctgc
     5161 gcgatgacgc ggcggtgcag ctcgtcctcg atacgcaaaa gcttctgcgt ctcctcctcc
     5221 ttgagcgaga cgacggggat gcccgtccag cgcgcgatga cctcctcgat gtcgtctctg
     5281 gtgacctcga cggtcagaag catctcctct tcgtcgtcgg gcagacgcaa ggaggagggc
     5341 acgcccttcg acgggtagcc ggacgacgcg gaggaggagc cgcgctcgcc gaacacgtcg
     5401 ccgaagacga cgccgcccgc gtcaccgccg cccacggggt cgcggcgcgc acgcagcttg
     5461 acgcgcgcgc cggcctcgtc gatcacgtcg atggccttgt cggggaggaa gcggtcgggg
     5521 atgtatcggc tcgactggta gacggccgcc tcgatggcgt cgtccgtgta gcgcacctga
     5581 tggaaggcct cgtaacgctc gcgcacgccg tcgagaatct ggacggcctc ttcctccgtg
     5641 ggcggcggca ccttgacggc ctggaagcgt cgctcaagcg acctgtcctt ctcaatcgac
     5701 ttgcggaact cggccggcgt cgtcgcgccg atgcactgta tctcgccgcg cgaaagcgcc
     5761 ggcttgagga tgttcgcggc gtcgagcgac ccttcggccg agccggcgcc gacgagcgtg
     5821 tgcagctcgt cgatgaagac gatgtactgc gggttctcga ccagctcgcg catgatctgc
     5881 ttgaggcgct cctcgaactg cccgcgatac ttcgtccccg ccacgatcag cgagaggtcg
     5941 agcgacagta tccgcttgtt ctccagaaac gtcggcacct cgccgcgcac gatgcgctgc
     6001 gccagtccct cgacgatggc cgtcttgccg acgcccggct cgccgatgag gacggggtta
     6061 tttttggtgc ggcggcaaag tatctggacg acgcgctcga tctcgccctc gcggccgacg
     6121 agcgggtcga gcttatcgtt ggcggcgtcg tcggtgaggt cgcgcgagaa ctcggcgagg
     6181 tgcggcgtct ctttcttctg cgcggcgcgc gggtcaacgc ccgtcgcgcg ggcgacctcc
     6241 tcgcgcacgg cgtggagctt gagtccgcgc tcgtagagaa tctcggcggc catcgagcgc
     6301 tcttcgcgca ggaggccgag caggaggtgc tcggtgccga tgtggcggtg ctggaggcgg
     6361 tcagactcct cgtgggcgta gtggagcacg cgcttggtct cgggcgcgag cggcagctcg
     6421 accgaggtgg aaatcttctc gcgcaggggc gcccgcccct cgatctcctt gcggatggct
     6481 tccagagacg cctgcgcgcg cgccaaaaag cgcgacgtga gcgttttgtc ttcgcgcatc
     6541 agtccgagca gcaagtgctc cggctcgatg gccggggcgc cgaactgact ggcctcgtag
     6601 cgcgcaaaga agatgacccg acgcgccttc tccgtgtagc gttcaaacat aaggcttcga
     6661 ctgtacttga ttaagagccg gttgcgacaa aaaaatcggc gagccggagg ttccggaaat
     6721 cagaccctac aggacgccgc ccgccggcat tataacccat gcccggcgaa gtcagaagag
     6781 ttgttcacaa ctcggctcaa aatccgcgcc ctccgcgcct ctgcgtcttc tgcgttaaat
     6841 cttcctcctg taataatgaa gtctgacgca gaagacgcag aagacgcgga gggcgcggag
     6901 gaagatgcgg aggcgagaag caccgtcgcc gctcagcggc ccgtgagacg cagcgacccg
     6961 aagtacttat cgaatttcgc gtcggtgaag gccgagcgcg gccccgtcat ggcgacgatg
     7021 aggagtgagc ggtcaacgac gaaagcgcgc gcgcgcgcgg tggtcggccg agctgaggtc
     7081 gaactcgcgc ccggcgactc cgtcgagcgc ggtcggctgc tgctccgaga gttcgagctt
     7141 catgtgcggc cattcgttgc gcgccgacgc ggggaggctc cccgcgagcg aagcgaggaa
     7201 gtcctgctcg aagcccctta cctcgaagag cgccgcggga atctgccccg tgcgcgtaac
     7261 ctcatacacg atgccgtcgg ccttcaccac gtatctgcgc gagcgcgcgt cggccagccc
     7321 acccgcgcgc ttctccgccg ccacatccac gggcttgccc ggcatcgcca cggagaaccc
     7381 ttcgccctcg ggcgcaaacg tcggccagcg ctcacctgcc gcgcgctcca gcgtcgaagg
     7441 cagagacata ttgtcgagac cgccgccgcc ggtgcgtcgc ccctgcgact gctgcgccga
     7501 cacggccgac accgccccgc acagacacgc aaacgccagc gcggcgcgca aaaatctctt
     7561 gtccatcggt cttaactcct tcggaagctt cggtgtgtgg aacccaggcg tgaggccggc
     7621 ggcggggcga agcgcgcgcg gccgacccgg cg
//
LOCUS       DASVES010000156         4719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_495077, whole genome shotgun sequence.
ACCESSION   DASVES010000156 DASVES010000000
VERSION     DASVES010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495077"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..921)
                     /locus_tag="VF240_06330"
     CDS             complement(154..921)
                     /locus_tag="VF240_06330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7174883.1"
                     /translation="MEGVSRPFYGEFAWAYDLLIAPPVASQCGVIGELFARRGVGAEA
                     LVLDAGCGTGRYAWELARRGYRVAGIDLSAALLAEARRRMVEGGVRVTLARGSVLALP
                     FAPRLDAVLCRGVLNDLLDDGSRGEAFASFAGVLRRGGVLVCDVREWGETVRRKTREP
                     VSEKVLETPRGRLTFRSETRLDEAGRRLLVSERHALAGDAGEVVAEYEFVMRCWTREE
                     LGRRLTEAGFGDVEYFGGYDLTPAGASDRLVCVSSRM"
     gene            1083..2300
                     /locus_tag="VF240_06335"
     CDS             1083..2300
                     /locus_tag="VF240_06335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7174884.1"
                     /translation="MYDAIIVGARCAGSPLAMLLARKGHRVLLLDKATFPSDTISTHH
                     IHQPGVARLRRWGVLEKVRASNCPPTTEMRFDVGPFALVGTPPPAEGNAEAYAPRRRV
                     LDEILADAAVEVGAELREGFSVEELTTGEGVVTGVRGRDRRGSAVTEKARIVVGADGA
                     RSLVARSVQAPIYLDRGMHTCNYYSYWSGVQLDAVELYSREGRMIVADKTNDGLTMVT
                     VVWPKEEFNRVRSNIEVEFMRSLDEHAPTLAERLRAGRREERFAGSGFLPNFFRKPYG
                     EGWALVGDAGYVKDPITAQGITNSFSHAEMLAEALDESLSGRREMGEALAGYERKRNR
                     EVLAMFEHTCQLARLAPPPPEMIQLLEALRGDQTETGRFLGTVTGTAPIQEFFSPENV
                     GRIINASALMPAA"
     gene            complement(2450..3532)
                     /locus_tag="VF240_06340"
     CDS             complement(2450..3532)
                     /locus_tag="VF240_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174885.1"
                     /translation="MSTTQSDPSGAELLLTPPDIYRAAAEGVLPRADAERLVQWGYEQ
                     RFNRSLVPEPKSPAPEQRRGLNLVTVAYYFGAMLMISACAWFLGDKWEALGAPGVFIT
                     VLIYMLLAARLGWWLRGKGYLVGGGLLITVAVCLVPLLVYTIEDMLGLWPGSDPGGYK
                     NYYPLIHGSWIVMELATIAAAAVALRFVRFGFLTAPMAFSFWFLSMDLAALILGDGNY
                     GWETRKWVSVIVGLCTLLIGYALDKKLHASDRPDSEDYAFWCYLFGMLAFWGGLTAMD
                     SGSEVKRALYALVNVGLIGLAVKLRRTTFLIFGALGVHLYLGHLAYRVFKDSFLFPFA
                     LALLGLSLILLTVWMQRRMLPRGGDA"
     gene            complement(3609..3827)
                     /locus_tag="VF240_06345"
     CDS             complement(3609..3827)
                     /locus_tag="VF240_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HEX7174886.1"
                     /translation="MSAKETMVERKVTYTLEKDGKFIIIENVPARVCVETGEQFFSPK
                     TVERLQQMIWEGEKPKKVIETPVYEFAA"
     gene            complement(3943..4152)
                     /locus_tag="VF240_06350"
     CDS             complement(3943..4152)
                     /locus_tag="VF240_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174887.1"
                     /translation="MSEEQNKEDAEMLDEYDFSQGVVGKYARQYAEGTNIVLLDPDVA
                     KVFPNSEAVNQALRAIAEIIQQRSR"
     gene            complement(4133..4417)
                     /locus_tag="VF240_06355"
     CDS             complement(4133..4417)
                     /locus_tag="VF240_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX7174888.1"
                     /translation="MSLSFEWDEGKASANLAKHEVSFSEASTVFADPLSRTIPDPLHS
                     DDEGRFIIVGESAAGRALVVVHTHRGEAVRIISARKATPRERKDYERGTE"
BASE COUNT          987 a         1552 c         1311 g          869 t
ORIGIN      
        1 gcgaaagaaa atcgacgttg attatcctca acgtcgattt tctttcgccc ctccggggct
       61 tcaattgttt gctcgtcgca taccacggga cggtcgcccg tggctacaat ctgccgccgc
      121 tccgcggctc gctttcgcgc aggacgatct tactcacatg cgcgaggata cacacaccag
      181 tcgatcactc gcgccggccg gtgtcaggtc ataaccgccg aagtattcaa cgtccccaaa
      241 ccccgcctcg gtgagacgcc gccccagctc ttctcgcgtc caacaacgca tgacgaactc
      301 gtactcggcg accacctcgc ccgcgtcgcc tgcgagtgcg tgccgctccg acaccaacag
      361 ccgccgcccc gcctcatcca gccgcgtctc gctgcggaaa gtcagccgcc ctcgcggcgt
      421 ctccaacacc ttctccgaca ccggctcccg cgtcttgcgc ctcaccgtct caccccactc
      481 gcgcacgtcg cagacgagca ccccgccccg ccgcaacact cccgcgaacg acgcgaaagc
      541 ctccccgcga ctcccatcat ccaacagatc attaagcacc cctcggcaca acacggcgtc
      601 gagccgcggc gcgaaaggca acgccagaac actcccgcgc gccagcgtca cgcgcacgcc
      661 gccctcaacc atccgccgac gcgcctccgc gagcagcgcc gccgacaaat caatcccagc
      721 gacccggtag ccacggcgcg caagttccca cgcatatctg cccgtcccgc accccgcgtc
      781 gagcaccaac gcctctgccc caacgccgcg acgcgcgaac agttcaccaa tcaccccgca
      841 ttgactggcg accggcggcg cgatgagcag gtcatacgcc cacgcaaact ctccgtagaa
      901 tggcctggat acaccttcca atgcttcctg cccagtaaaa gccgctatac gctttgtgaa
      961 caccagcctg caaaaatttt tgattctaat tttgctggag ttgattctct ccgacgcaag
     1021 tttacgcgtt aattgatgtg ggaaggtaat ccttccagct ctggttgcag aggaggttcg
     1081 acatgtacga cgcgattatc gtcggcgcgc gctgcgcggg ctctccgctg gcgatgctgc
     1141 tcgcccgcaa aggccaccgg gtattactgc tggataaggc cactttcccc agcgacacga
     1201 tctccacaca ccacatacac cagcccggcg tggcgcggct caggcgctgg ggggtgctcg
     1261 aaaaggttcg agcttcgaac tgccccccga cgaccgagat gaggttcgat gtcggcccct
     1321 tcgcgctcgt gggcacgccg cccccggccg agggcaacgc ggaagcctac gccccgcgcc
     1381 gcagggtgct cgacgagatt ttagccgacg cggctgtgga ggtcggcgcc gagctgcgcg
     1441 aaggtttttc cgtcgaagaa ctcactaccg gcgagggggt ggtgacgggc gtccgcggtc
     1501 gggacagaag gggttcggcc gtgacggaga aggcgcgcat cgtcgtcggc gcagacggcg
     1561 cacgctcgct tgtcgcgcgc agcgttcagg cccccatcta cctcgaccgg gggatgcata
     1621 cctgcaacta ctactcctac tggagcggcg tgcagctcga cgcggtcgag ctttactcgc
     1681 gcgaaggccg gatgatagtg gccgacaaga ccaacgacgg gctgaccatg gtcaccgtcg
     1741 tgtggccgaa agaggagttc aaccgcgtgc ggtcgaacat cgaagtcgag ttcatgcgct
     1801 ccttggacga gcacgcgccc acgctggccg agcgcctgcg cgccggccgc cgtgaagagc
     1861 ggttcgccgg gtcgggcttc ctgcccaact tcttccgcaa gccgtatggt gagggttggg
     1921 cgctcgtcgg ggacgcgggt tatgtcaaag acccgatcac cgcgcagggt attacaaaca
     1981 gtttcagcca cgccgagatg ctcgccgaag ccctcgacga aagcctctcg gggaggcggg
     2041 agatgggcga ggcgctcgcc ggttacgagc ggaagcgcaa ccgcgaggtg ttggcgatgt
     2101 tcgagcacac ctgccaactc gcccggctcg cgccgccgcc gcccgaaatg attcagctcc
     2161 tcgaagccct ccggggcgat cagaccgaga cgggacgctt cctcggcacg gtcacgggca
     2221 cggcgccgat acaggagttc ttctcgccgg agaacgtcgg ccgcatcatc aacgcgtcgg
     2281 cactcatgcc cgcggcataa aggagcgcgc gcagaggtag aatcggccta aagcgtaaca
     2341 aatgcgacgc aacataacgc ccgaataaac cggcgcgcac gtgcctctga aaccacccga
     2401 gctatcgatg agcggctggc taattgcgcg tccggccgaa atgattttgt caggcgtcgc
     2461 cgccgcgcgg caacatgcgg cgctgcatcc acactgttaa gaggatgaga ctcaacccca
     2521 gcagcgcgag cgcgaacggg aagagaaacg agtccttgaa gacccgatag gccagatgtc
     2581 cgagatacag atgcacgccg agcgcgccga aaatcagaaa ggtcgtccgc cgcaacttca
     2641 ctgccagccc aatcaacccg acgttcacca acgcgtaaag cgcacgcttc acctccgacc
     2701 cgctatccat cgcggtcagc ccgccccaga aagccaacat cccgaacagg tagcaccaga
     2761 acgcataatc ttccgagtcg ggtctgtccg acgcgtgtaa tttcttatcg agggcgtagc
     2821 caatcagcag tgtgcacagg cccacgatca cgctgaccca tttgcgcgtc tcccaaccat
     2881 aattcccatc ccccagaatc aacgccgcca agtccatcga aagaaaccag aacgaaaacg
     2941 ccatcggcgc ggtaagaaaa ccaaatcgta caaagcgcag ggcgacggcc gcggccgcga
     3001 ttgtggccaa ctccatgaca atccacgagc cgtggatcag cgggtagtaa ttcttgtagc
     3061 cccccggatc actgccgggc cagaggccga gcatgtcctc aatcgtgtag acgagcaacg
     3121 gcacgagaca cacggccaca gtaatgagca ggccgccccc cactaaatag cccttgccgc
     3181 ggagccacca tcccagtcgc gccgccagca acatgtagat cagaacagtg atgaaaactc
     3241 cgggcgcgcc cagcgcctcc cacttatcgc cgaggaacca ggcgcacgct gaaatcatca
     3301 gcatcgcgcc gaagtagtag gcgaccgtca ccagattgag acccctgcgc tgctccggcg
     3361 ccggcgactt cggctcgggt acgagcgagc gattaaagcg ctgttcataa ccccactgca
     3421 caaggcgctc ggcgtccgct cgcggcagta cgccttccgc cgcggcgcgg taaatatctg
     3481 gtggcgtgag caggagttcc gcaccgcttg ggtcagactg agtcgtggac ataacgctca
     3541 cttacaacga tggaagagat gctgtcgagg cgtcgggttg aaggagttgt tagcctgcgc
     3601 gtgaggattt atgcggcaaa ttcataaact ggggtctcga tgactttctt gggcttttcc
     3661 ccttcccaaa tcatctgctg taaccgttcg actgttttcg gcgaaaagaa ttgctcgcca
     3721 gtttcgacac acactctggc aggtacgttc tcgatgataa tgaatttccc atctttttca
     3781 agcgtatatg tgacttttcg ctcgaccatt gtttctttgg cactcatctc agctacttcc
     3841 tcaccttaaa atcaatcaca agccatagta caggacgcaa ggtcgatgag agccaagctg
     3901 agggcggccg ggtgcatgcc gagcgttaga tgctggggca cattagcgag accgctgctg
     3961 gatgatttca gctatcgcgc gtagggcttg attaaccgcc tcagagttcg ggaatacttt
     4021 agccacatca gggtcgagta acacgatatt cgtcccctcg gcatactgcc tggcgtattt
     4081 cccgaccacg ccctggctga agtcatactc gtctaacatc tcggcgtctt ctttattctg
     4141 ttcctcgctc ataatctttc ctctctcggg gcgtcgcctt ccgcgcgctg atgattctga
     4201 cagcctctcc gcggtgggtg tgtaccacca ctaaggcgcg ccccgccgcc gactctccta
     4261 cgatgatgaa tctcccctca tcatccgagt gtagcgggtc ggggatcgtt ctcgacagcg
     4321 ggtcggcgaa cacggttgac gcctcggaga aagagacctc gtgcttcgcg agattcgccg
     4381 aggctttccc ctcgtcccac tcgaacgaca ggctcatggc tctgaatata cgatgaatcg
     4441 ggtgatggag gaaggtatct aacgcccggc atcacccgcc gcgcacgcaa ctgcagtacg
     4501 gccaagctaa cgatgagagg caagctaact cgcggtcggg ttgcatgccg ttgttaggct
     4561 tcggggaatg atttgtcagg ttaattattt ccggttaagg gggcggaggt attgccgtca
     4621 atgggtctaa atttttccaa tggatatatc tcgctgacat cgctctacca tgcctttgaa
     4681 ttggatgatt cttgtcttcc gaattagccc agccaatca
//
LOCUS       DASVES010000157         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1329897, whole genome shotgun sequence.
ACCESSION   DASVES010000157 DASVES010000000
VERSION     DASVES010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329897"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VF240_06360"
     CDS             complement(<1..1461)
                     /locus_tag="VF240_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174889.1"
                     /translation="MSSMKCPQCGLVNWVSAEACKRCKLPFVTSAPAAPAVTPPDADE
                     QAPSAADEAHPPAASAEAPAETAPVQAGPQQQPYIYDARTTHGHAHAPWRSPYDVSGR
                     DETDEAVNTLDIDFEVAPFIDTTMTIRDTYALSRSQFRLIARIVLLAIAPHALLILAV
                     GTSFATESPTFPAGMPFLFGAAPPSQTMGHPDFTGVLSFFMLTLIGYYFVRWAVMPSA
                     LIYGLVTSLNTGESPSLLECYRWGFRRSISSGLSLLLCVVLTFIGFVLLIVPGIYLAV
                     CFTLVMPIAAIEGCGAIEVMRRSWKLSRGRRGAIFLSSFGWGVLIFLFTLLATLFLTL
                     VAGVLRSSVVGSVATLLVTEMLGATGIVLSLVIYLGIAHYGRAESGGDAAYGQPPPTG
                     DMGGSFSGGFVPQPGGWGTPQPAFAGPGGYASAGAGSGAQKTTIVALVVGGVLVMLAF
                     VGIIAAIAVPNLLASRRAANEASAIANLRQLASAEMT"
     gene            complement(1530..2543)
                     /locus_tag="VF240_06365"
     CDS             complement(1530..2543)
                     /locus_tag="VF240_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174890.1"
                     /translation="MRFPKAYRASGVRLAAVAASLCAFGLAGVAHAQTPADRMAQSPA
                     AVTATQQAAPARETEMQCSGFIEHRPPAGRLEIVGAEEEQEQHIFGEGDYVYINGGAQ
                     AGVRAGQEFSVIRPRGRFKTRFSSKDGSLGMYTQELGRLRVVNVKPNVSVALVTQSCD
                     NMLFGDLLREVPVRSAPIVRAELPLERFADPSGKQQGRIVLSRDNRELMSKDSVVYID
                     LGVEDNVKPGDRLTIYRPLGVGAISRFKDKEVTPGGSRSFESPIFRSGKHSNKAQRVK
                     DPVDGHYKSTTDTPAIMSRRPAMPRKIVGEVVIISVEQRTAAAIITRYAQEIHTGDFV
                     EVQ"
     gene            2884..2956
                     /locus_tag="VF240_06370"
     tRNA            2884..2956
                     /locus_tag="VF240_06370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2917..2919,aa:Thr,seq:tgt)
     gene            2980..3067
                     /locus_tag="VF240_06375"
     tRNA            2980..3067
                     /locus_tag="VF240_06375"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3014..3016,aa:Tyr,seq:gta)
     gene            3165..3240
                     /locus_tag="VF240_06380"
     tRNA            3165..3240
                     /locus_tag="VF240_06380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3198..3200,aa:Gly,seq:tcc)
     gene            3280..3351
                     /locus_tag="VF240_06385"
     tRNA            3280..3351
                     /locus_tag="VF240_06385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3312..3314,aa:Thr,seq:ggt)
     gene            3397..>3876
                     /locus_tag="VF240_06390"
     CDS             3397..>3876
                     /locus_tag="VF240_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7174891.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAKHNPKIT
                     VRAFDSIDNAPEEKARGITIATAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPAMVVFMNKVDAVDDAELLDLVELEVRELLS
                     "
BASE COUNT          706 a         1174 c         1329 g          667 t
ORIGIN      
        1 agtcatctcg gccgaggcca actgtcgcag gttcgcgatg gccgaagcct cgttggcggc
       61 gcggcgcgac gcgagcaggt tggggacggc gatggctgcg atgataccca cgaacgccaa
      121 catgacgagc acgccgccca cgacgagcgc cacgatggtc gtcttttgcg ccccgctgcc
      181 cgcgcccgcg gaggcgtagc cgcccggccc cgcgaaagcc ggttgcggcg tcccccagcc
      241 gccgggctgc ggaacgaacc cacctgagaa agagcctccc atgtcgcccg tcggcggcgg
      301 ctggccgtac gcggcgtcgc cgccgctctc ggcccgtcca tagtgggcga tgccgaggta
      361 gatgacgagg gagaggacga ttccggtggc acccagcatt tcggtgacga ggagcgtggc
      421 gacggagccg acgacgctgc tgcggagcac gcccgcgacg agggtcagga agagtgtggc
      481 gaggagcgtg aagaggaaga tgagcacgcc ccacccgaag ctcgaaagga agatcgcccc
      541 gcgccgcccg cgcgagagct tccaactccg ccgcatgacc tcgatggctc cgcacccctc
      601 gatggccgcg atgggcatga ccaacgtgaa gcagaccgcg aggtagatgc cggggacgat
      661 caggagcacg aatccgatga acgtcaggac gacgcacaac agcagcgaca gccccgacga
      721 gatcgaccgg cgaaagcccc agcgataaca ctctaacagg ctcggcgact cgcccgtgtt
      781 gagcgaagtc accagcccgt agatcagggc cgagggcatc acggcccagc gcacgaagta
      841 gtagccgatg agcgtcagca tgaagaagct gaggacgccg gtgaagtccg ggtggcccat
      901 cgtctgggag ggcggcgcgg cgccgaagag gaacggcatc cctgcgggga atgtcgggga
      961 ctcggtggcg aagctcgtgc cgacggcgag aatcagtagc gcgtgcggcg cgatggccag
     1021 cagcacgatg cgcgcgatga gacggaactg cgagcgcgac agcgcgtagg tgtcgcggat
     1081 ggtcatggtc gtgtcgatga acggcgcgac ctcgaagtcg atgtcgagag tgttgacggc
     1141 ctcgtccgtc tcgtcgcgtc cgctcacgtc gtagggcgag cgccaagggg cgtgcgcgtg
     1201 tccgtgcgtt gtccgcgcgt cgtaaatgta cggctgctgc tgcggcccgg cttgaaccgg
     1261 cgccgtctcc gcgggcgctt cggccgaggc tgcgggagga tgagcttcgt cagccgcgga
     1321 cggggcctgt tcgtccgcgt ccggcggcgt gacggccgga gccgccggag cggaggtgac
     1381 gaagggcagc ttgcagcgtt tacacgcctc ggccgacacc cagttgacca gcccgcactg
     1441 cggacatttc atgctgctca tagtctcctc cgcgcagggc atggtgagag acccgcgcgt
     1501 cacggacgcg cccggtgcgg ggcgagtcgc tactgaactt ccacgaagtc gcccgtgtgt
     1561 atctcctgtg cgtaacgcgt gatgatggcc gcggccgtgc gctgctcgac gctgatgatg
     1621 acgacttcgc cgacgatctt gcggggcatc gccgggcggc gcgacatgat ggcgggcgtg
     1681 tcggtggttg acttgtagtg gccgtccacc gggtctttca cgcgctgcgc cttgttcgag
     1741 tgcttgccgc tgcggaagat ggggctctcg aagctgcggc tgccgcccgg tgtgacttct
     1801 ttatctttga agcgcgagat ggcgcccacg ccgagggggc ggtagatcgt cagacggtcg
     1861 ccgggcttga cgttgtcctc cacgcccaaa tcgatgtaga cgaccgagtc cttcgacatc
     1921 aactcacggt tgtcgcgcga caggacgatg cggccctgct gcttgccgct cggatcggcg
     1981 aagcgttcga gcggcaactc ggcgcggacg attggcgcgc tccgcaccgg cacttcacgc
     2041 aggaggtcgc cgaacagcat gttgtcgcac gactgcgtga cgagcgcgac cgacacgttc
     2101 ggcttcacgt tgacgacgcg cagccgcccc agttcctgcg tgtacatccc gagcgagccg
     2161 tccttgctac tgaagcgggt cttgaagcgg ccccgcgggc ggatgaccga aaattcctgc
     2221 ccggcccgga cgccggcctg cgcgccgccg ttgatgtaga cgtagtcgcc ttcgccgaag
     2281 atatgctgct cctgctcttc ctccgcgccg acgatctcca gacgaccggc cggcggccgg
     2341 tgctcgatga accccgagca ttgcatctcg gtctcgcgcg ccggcgcggc ctgctgtgtc
     2401 gccgtgacgg ctgcggggct ttgggccatc ctgtcagcgg gcgtctgcgc gtgggccaca
     2461 ccggcgaggc cgaaggcgca gagggaggcg gccacagccg cgaggcgcac gccggaggcg
     2521 cggtaagctt tagggaagcg cacgtgtgaa ttctccttgt gaattacgtg atttcagagg
     2581 gccatcgagg tcttgaaggg tgtgggaagg ccgtttgatt cggacttcga cgaatgacgg
     2641 ggacggggca aacttctgtg aggggccggc aggcaggcac tgaaagtggc cgaggcaccg
     2701 cccggcggcc taaaatcatc ctactgtctg ccacgccgga gcgtcaatat ttttcttcaa
     2761 tttagccgcg attgaccccg aaaatgcccg cacggggcgg gtgcggcctg cggcttgcgt
     2821 tttcgcggat gtgttggtag tattcgagtt ttgtcggtag cggcaaatct tttactgtag
     2881 cgggccggcg tagctcagtt ggtagagcat ctgatttgta atcagagggt cgggggttcg
     2941 agtcccttcg ccggctcctt caaatctttg aaagttccgg caggggtggt cgagtggtta
     3001 atggcaccgg gctgtaaacc cggccggcga aagccgtacg taggttcgaa tcctaccccc
     3061 tgcaccagta agccaagagg ctagaggtca gaggttagag gtcagtaaaa acggattgtc
     3121 ttttactgac ctctaacctc tgacctctga cctctcgcat tcgggcggga gtagctcagt
     3181 tggtagagca tcagccttcc aagctgaggg tcgcgagttc gagcctcgtc tcccgctcca
     3241 ttttctgaca gttaggatac gagtcgagcg cctgcgagcg cccgagtagc tcaggggtag
     3301 agcgcctcct tggtaaggag gaggtcatgg gttcaaatcc catctcgggc tttcggatag
     3361 acacaggaat ctttaaacga agaggctatt taaggaatgt ctaaggagaa atttgatcgg
     3421 tcgaagccgc acgtgaacat cgggaccatc gggcacgtcg atcacgggaa gacgaccttg
     3481 acggcggcga tcacgaaggt tttggccaag cacaacccga agatcacggt gcgggccttt
     3541 gactccatcg acaacgcgcc cgaggagaag gcccgaggga tcaccatcgc cacggcccac
     3601 gtcgagtacg agacggccaa ccgccactac gcgcacgtcg attgtccggg gcacgccgac
     3661 tacgtcaaga acatgatcac gggggcggcc cagatggacg gggcgatcct cgtcgtcgcg
     3721 gccaccgacg gccccatgcc gcagaccaga gagcacatcc ttttggcccg gcaggtgggc
     3781 gtgccggcga tggtcgtctt tatgaacaag gtcgacgccg tcgatgacgc cgaactcctc
     3841 gacctggtgg agttggaggt cagagaactg ctttcg
//
LOCUS       DASVES010000158         7267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_22695, whole genome shotgun sequence.
ACCESSION   DASVES010000158 DASVES010000000
VERSION     DASVES010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22695"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VF240_06395"
     CDS             complement(<1..333)
                     /locus_tag="VF240_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7174892.1"
                     /translation="MRDELRVDFRVEGRLVQTRLNRVVSADGGKVVQVQPKIMDVLVR
                     LAERPGEVVTREELFSTVWAGTHVTEHVLARAISELRKVFDDSAQKPRVIETIPKVGY
                     RLIASVTAA"
     gene            699..2018
                     /locus_tag="VF240_06400"
     CDS             699..2018
                     /locus_tag="VF240_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7174893.1"
                     /translation="MKRLKRTAPAILVCLLSASTLMRPAPEAAASGRIAAAAPTAHPS
                     VQRFVGGLWFDGRTFAPRTVYSVNGVFRSSHAGAVDATHDLSGKFVVPPFAEAHNHHF
                     MEGMDYRSQIAAHLAQGIFYAKNSNTMQRLTDPVRPHVNTPESVDVLFAGGGLTATGG
                     HPVQIYDHIADRKMFPGWGREEMKNQAYFIIDTERDLEDKWPLIKAGRPDFIKTYLEY
                     SEEYERRKSDPKFYGQRGLDPKLLPKIVAKAHRERLRVAVHVNTAADFRNALSAGADE
                     IAHLPLAPLTEEDARRAARQGTVVVTTTLSHRRTDHVTDLDGVHRHNLRLLHAAGVRL
                     AVGTDDTNRTALDEAMNLARLGVFDNATLLRLWTEVTPQAIFPARKLGLLKDGYEASF
                     LALNEDPLENFANVKRIAVRFKQGHNISPAAHPERPGGDAGKPANHP"
     gene            2322..4139
                     /locus_tag="VF240_06405"
     CDS             2322..4139
                     /locus_tag="VF240_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7174894.1"
                     /translation="MSFSSLFRKKPIELIQRDAELGLGDHGELEREHPHLRRTLGVTD
                     LTAMGIAAIIGAGIFSTIGNAAFNGGPAVAFLFVFTAIACAFSALCYAEFASKIPVAG
                     SAYTYAYASFGELIAWIIGWDLLMEYAIGNIAVAISWSDYFTSLLSSIRIPSLGIDGI
                     HLPAWMTMDFLTASRGYEKVVETLGSAATPAAIAASTDIARYGDAFAAWTTAPALGGL
                     RLVADIPALVIVFLVTVLVYVGIRESKRASNIMVVFKLSIILIVIVVGAFYINTDNWN
                     PFAPNGIGGVLKGVSGVFFAYIGFDAITTTAEECKNPQRDLPLGMIYSLVICTVLYIL
                     IALVLTGMVNYTLLQVGDPLAFVFGPEGANLPWLRGIVAVSAVVAMTTVLLVFQLGQP
                     RIWMAMSRDGLLPKLFSAIHPKYKTPWFATIVTGLLVAIPALFMNLTEVTDLTSIGTL
                     FAFVLVCGGVLLINRGGRQPGKYVPYIDSRFVVPAMYVGAWLIFFVFYRETLVNYFSF
                     TDTVEGQLVSGWEVFKHEIPMLIYIVLAGALAVLCFVKRLSLIPVMGLLTCGYLMTEL
                     GLTNWIRFIVWLAVGLVLYFLYGYRHSRLAERIDPERVD"
     gene            complement(4378..5496)
                     /locus_tag="VF240_06410"
     CDS             complement(4378..5496)
                     /locus_tag="VF240_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX7174895.1"
                     /translation="MQHSLKEIVESYDARAPLEEAWTIPAPWYTDARVYELERRTVFA
                     RTWQLVGRAEQVGEPGQYVTCEIAGEPVVVVRGGDGALRAFFNVCRHHAAAVLTEPEG
                     RAASLRCPYHGWTYTLEGALRGTPDFAGVCQFERGENGLVPVECGVWENWVFVKVESG
                     GPALEEFLGAELIEQARPLKLSSLRWMERRHYSFDCNWKVFVDNYLDGGYHVPYLHKG
                     LDSVLDYSNYTIENGERFCLQSSPVVSAGAEAQTGAVRRGERALYYWIYPNFMVNWYE
                     GVMDTNLVLPRGVDQTEVIFDFYFPDVSEAARERNRASIDVGQRIQDEDTAICKSVQR
                     GLASRAYGAGRLSVRREAGEHLFHRLLYADLKAGLEDE"
     gene            complement(5631..6833)
                     /locus_tag="VF240_06415"
     CDS             complement(5631..6833)
                     /locus_tag="VF240_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX7174896.1"
                     /translation="MPIGTAFHERTFALCESLSYREWSGYYAVSSYEPHHEHEYNAIR
                     NAAALIDISPLYKYRLTGRDATRLVDRIITRDMRKVSVGQVVYTPWCDERGKVIDDGT
                     VSRLGENLYRWTAADPSLRWFTMNAAGMDVEIEDISEETAALALQGPTSGRLLKSLVR
                     GCDIESLKYFRVESGEIADVPVDISRTGYTGDLGYEIWMPMELAVHVWDALMEGGRAF
                     DIHPAGMLALDVARIEAGLLLIDVDFHGSKKALIESQKYTPYEMGLGRLVQLDKARFV
                     GQAALAEEHRRGHAREIVGLEVDWTGVERLYEAAGLPPSISPIASRVAVPVYREGAQT
                     GRATSTTWSPTLKQFIALATIRREHARPGTRLEVEVTVEAVRHRVGATVVKTPFFNPK
                     RKTATPVV"
     gene            6914..>7267
                     /locus_tag="VF240_06420"
     CDS             6914..>7267
                     /locus_tag="VF240_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7174897.1"
                     /translation="MAQKYDVIVIGGGHNGLVCAAYLARTGKKVLVLERRHVLGGAAV
                     TEEVFPGFKFSVASYVVSLLRPEIIRELDLPRHGLEILPLDGTFTPMLNGDYLWRTND
                     HAKTRREIARHSKLDA"
BASE COUNT         1265 a         2560 c         2147 g         1295 t
ORIGIN      
        1 ggcggcggtg acgctcgcga tgagacggta gccgaccttc gggatggtct cgatgacgcg
       61 gggcttttgc gcgctgtcgt cgaagacctt acgcagctcg gagatggcgc gcgccagcac
      121 gtgctccgtg acgtgcgtgc cggcccagac ggtgctgaac aactcctccc tcgtgacgac
      181 ctcgcccggc cgctcagcca gacgcacgag cacgtccatg atcttcggct gcacctgcac
      241 gaccttgcca ccgtcggccg agacgacccg gttcagacgc gtctgaacca gacgaccttc
      301 gacccggaaa tctacacgca gttcatcccg catctttccc tccctcttct tccctttaac
      361 aatcgacccc ggctcattgg gcgtgacttg gacgccggag tttccagact tgccgggcta
      421 tacgaccgcc gccgcgcggc agttcggacg gcaaatgccg aacagtaccg cgagcggtag
      481 cgagcgggta tgcctgtgat gagaggcccg tatcgcgtat ccagacccgc gaggcttccg
      541 ctcgcggtac tgccccggac acgcagtcgt ccccacggac ggccgcgcgc ggcaaagcct
      601 cagataaaaa tcagagaaag atcaagggtc gccgccggcc gcgtggcgta gaacgtggcg
      661 agtcgtcaaa cccgtcccgc ccgaaaagga gatcaacagt gaaacgtctg aagcgaacgg
      721 cccccgccat ccttgtctgc ctgctgagcg cgtccactct gatgcggccc gcgcccgaag
      781 ccgccgcctc ggggcgcatc gccgccgccg ctccgaccgc gcacccgtcc gtccaacgct
      841 tcgtcggcgg cctgtggttc gacggccgca cgtttgcgcc gcgcaccgtc tactcggtca
      901 acggcgtctt ccgctcaagc cacgccggag cagttgacgc cacgcacgac ctgagcggca
      961 agttcgtcgt cccgcccttc gccgaagcgc acaaccatca cttcatggaa ggcatggact
     1021 accgctcgca aatcgccgca cacctcgcgc agggcatctt ctacgcgaag aactcgaaca
     1081 ccatgcagcg gctcaccgac cccgtccgcc cacacgtgaa cacgcccgag agcgtcgatg
     1141 tccttttcgc gggcggcgga ctcaccgcga cgggcggcca ccccgtgcag atttacgacc
     1201 atatcgccga ccggaaaatg ttccccggct ggggccgcga ggagatgaag aatcaggctt
     1261 acttcatcat cgatacggag cgcgacctcg aagacaagtg gcccttaatc aaggcgggcc
     1321 gccccgactt catcaaaact tatctggaat actccgaaga gtatgagcgg cgcaaatcag
     1381 acccgaaatt ttacggccag cggggactcg acccgaagct tctgccgaag atagttgcga
     1441 aggcgcaccg cgagcgtctg cgcgtcgccg tccacgtcaa caccgcggcc gacttccgca
     1501 acgcgctctc ggccggcgcc gacgagatcg cccacctgcc gctcgcgccc ctcaccgaag
     1561 aggacgcgcg gcgcgcggcg cggcagggca cggtcgtcgt cacgaccact ctcagccacc
     1621 gccgcacaga ccacgtcaca gacctcgacg gcgtccaccg acacaacctg cgtctgctcc
     1681 acgcggccgg cgtgcgtctg gccgtcggca ccgacgacac caaccgcacc gcgctcgacg
     1741 aggccatgaa cctcgcgcgc ctcggcgtct tcgacaacgc caccctgctc cgtctctgga
     1801 cggaagtcac cccgcaggct atcttccccg ctcgcaaact cggcctgctc aaagacggct
     1861 acgaggcgag tttcctcgcg ctcaacgaag accccctcga aaacttcgcg aacgtcaaac
     1921 gcatcgccgt ccgcttcaag cagggccaca acatctcgcc ggccgcgcac cccgagcgtc
     1981 cgggcggaga cgcgggcaag ccggcgaatc atccgtgata cgcctctggc ccgatgctct
     2041 cacttgggtg taggtagagt tggactcacc gcagaggcgc ggaggacgca gagggggcgc
     2101 agagagttga ttctctccgc gtgaactctg cgtcctctgt gcctctgtgg tgaactgcct
     2161 cacccctacg ctcaagtttg agtcactctt aagggtgatg gccgaactta tctgttgcca
     2221 tcctcccacc ccctgtgaca cactccgcgc gtccacagtt ggctttcccc aaaacgctct
     2281 accaacaagt cttccgaaac gcgcgggcga ataaaaccga gatgagcttc tcctccctct
     2341 tccgcaaaaa acccatcgag ctgattcaac gcgacgccga actgggtctc ggcgaccacg
     2401 gcgaactcga acgcgagcac ccgcacctgc ggcgcacgct cggcgtcacc gacctcaccg
     2461 ccatgggcat cgccgccatc atcggcgccg gcatcttctc gaccatcggc aacgccgcct
     2521 tcaacggcgg cccggccgtc gccttcctct tcgtctttac ggccatcgcc tgcgccttct
     2581 ccgcgctctg ctacgccgag tttgcctcga agattcccgt cgccggctcc gcctacacct
     2641 acgcctacgc ctccttcggc gaactcatcg cgtggatcat cggctgggac ttgttaatgg
     2701 agtacgccat cggcaacatc gccgtcgcga tttcgtggag cgactacttc acgagtctcc
     2761 tgtcgagcat ccgcatcccg tcgctcggca tcgacggcat ccacctgccc gcgtggatga
     2821 cgatggactt cctgacggcc tcgcgcggct acgaaaaggt ggtcgaaacg ctcggctcag
     2881 ccgcgacgcc cgccgccatc gccgccagca cagacatcgc cagatatggc gacgccttcg
     2941 cggcgtggac gaccgcgccc gccctcggcg gactgcggct cgtcgccgac atcccggcgc
     3001 tcgtcatcgt cttcctcgtc accgtcttag tctacgtcgg catccgcgag tcgaagcgcg
     3061 cgtcgaacat aatggtggtc ttcaagctct cgatcatcct catcgtcatc gtcgtcggcg
     3121 ccttctacat caacaccgac aactggaatc cgttcgcgcc caacggcatc ggcggcgtac
     3181 tcaaaggcgt ctccggcgtc ttcttcgcct acatcggctt cgacgccatc acgaccacgg
     3241 ccgaggagtg caagaacccg caacgcgacc tgcccttggg catgatctac tcgctcgtca
     3301 tctgcacggt gctctacatc ctcatcgcgc tcgtcctgac ggggatggtc aactacacgc
     3361 tcctgcaagt cggcgacccg ctcgccttcg tcttcggccc cgagggcgcg aacctgccgt
     3421 ggctgcgcgg catcgtggcc gtctcggcgg tggtggcgat gacgacggtg ctcctcgtct
     3481 tccaactcgg acagccacgc atctggatgg cgatgagccg cgacgggctt ttgccgaagc
     3541 tcttctcggc catccacccg aagtacaaga cgccgtggtt tgcgaccatc gtcacgggcc
     3601 tgctcgtagc catccccgcg ctgttcatga acctcacgga agtcaccgac ctgacgagca
     3661 tcggcacgct cttcgccttc gtgctggtct gcggcggcgt gctgctgatt aaccgcggcg
     3721 gccggcagcc gggcaagtac gtcccctaca tcgactcgcg cttcgtggtg ccggccatgt
     3781 acgtcggcgc gtggctcatc ttcttcgtct tctaccgcga gacgctggtg aactatttca
     3841 gcttcacgga cacggtcgaa gggcagttgg tctcgggctg ggaggtcttc aagcacgaga
     3901 taccgatgct catctacatc gtcctcgccg gcgcgctcgc cgtgctctgc ttcgtcaagc
     3961 gtctgtcgct catccccgtg atgggccttc tgacctgcgg ctacctgatg accgaactgg
     4021 ggctgaccaa ctggatacgc ttcatcgtct ggctcgccgt cggcctcgtc ctctacttcc
     4081 tctacggcta ccgccacagc cgtctggccg agcgcatcga ccccgagagg gtggattaaa
     4141 caagtcgcca gcccccgcaa gggggcggaa gaatgtagcc cacggcgcga gccgtgggta
     4201 acggacagcc aggacaatga aagccccgga ggggcgaaag aacctcaagc ttgagcacga
     4261 tgctcgttga ggttctttcg cccctccggg gcttcgcttt tttattctcg tacattccca
     4321 cggctcgcgc cgtgggctac attcttccgc cgctccgcgg ctggattctg ccgccgctta
     4381 ttcgtcttcc agtcccgctt tcaggtctgc gtaaagtagt cgatggaaga ggtgctcgcc
     4441 ggcctcgcgg cggacggaga gacgcccggc cccgtaggcc cgcgacgcga ggccgcgctg
     4501 gactgacttg cagatggccg tgtcctcatc ctgaatcctc tgccccacgt cgatgctcgc
     4561 gcggttgcgc tcgcgcgccg cctcggacac gtcggggaag tagaaatcga agatgacctc
     4621 cgtctgatcg acgccgcgcg gcagcacgag attcgtgtcc atcacgccct cgtaccagtt
     4681 gaccatgaag ttcggataaa tccagtagta gagcgcgcgc tcgcccctcc tgaccgcgcc
     4741 ggtctgcgct tcggcccccg cgctcacgac gggactcgat tgcaaacaga accgctcgcc
     4801 attctcaatc gtgtagttcg agtagtcgag gacgctgtcg aggcccttgt ggaggtacgg
     4861 gacgtgatag ccgccgtcga ggtagttatc gacaaagacc ttccagttgc agtcgaacga
     4921 gtagtgacgc cgctccatcc agcgcagcga tgacagcttc agcgggcgcg cttgttcgat
     4981 caactccgcg ccaagaaact cttcgagcgc ggggccgccg ctctccacct tgacgaagac
     5041 ccagttctcc cagacgccgc actcgacggg gacgaggcca ttctctccgc gctcgaactg
     5101 gcagacgccg gcgaagtcgg gcgtgccgcg caaagcgcct tcgagcgtgt acgtccagcc
     5161 gtgatacggg cagcgcaggc tcgcggcgcg cccctccggc tcggtcaaca cggcggccgc
     5221 gtggtgccgg cagacgttga agaaagcgcg caatgccccg tcgcccccgc ggacgacgac
     5281 cacgggttcg ccggcaatct cacaggtgac gtattgcccc ggctccccga cctgctcggc
     5341 gcgcccgacg agctgccacg tccgcgcaaa caccgtccgc cgctccagct cgtagacgcg
     5401 cgcgtccgtg taccacggtg ccggtatcgt ccacgcctct tcaagcggcg cgcgcgcgtc
     5461 gtagctttcg acgatttcct taagcgaatg ctgcatggct gcacgatgta ggacaggcat
     5521 tcctgcctgt cttgagcgcg gagcgcgaaa aacttcgcgc gcacattcaa cttgagattc
     5581 ggcgaatccc tttcgcgctg acgcgctcac gacaggcagg aatgcctgtc ctacacgacc
     5641 ggcgtcgccg tcttgcgctt cgggttgaag aagggcgtct tcactaccgt cgcgccgacg
     5701 cggtggcgca cggcctcgac cgtcacctcc acttcaaggc gcgtccccgg ccgggcgtgc
     5761 tcgcgccgga tggtggcgag cgcgatgaac tgcttgagcg tgggcgacca cgtggtcgaa
     5821 gtcgcccgcc ccgtctgcgc gccctcgcgg tagacgggca cggccacgcg cgacgcgatg
     5881 ggcgagatcg acggcggcag ccccgccgct tcgtacagac gctcgacgcc cgtccaatcc
     5941 acttccagcc cgacgatctc gcgcgcgtgc ccgcgccggt gttcttcggc gagcgccgcc
     6001 tgcccgacga agcgcgcctt gtccaactgg acgagccggc ccaagcccat ctcgtagggc
     6061 gtgtacttct gcgactcgat gagcgccttc ttgctgccgt ggaagtccac gtcgatgagc
     6121 agcaggcccg cctcgatgcg cgccacgtcg agcgcgagca tcccggccgg gtggatgtcg
     6181 aaggcgcggc ccccctccat cagcgcgtcc cagacgtgga cggccagctc catgggcatc
     6241 cagatttcgt aaccgaggtc gcccgtgtag cccgtgcgcg agatatcgac gggcacgtcg
     6301 gcaatctctc ccgactcgac gcggaagtat ttcaagctct cgatgtcgca gccgcgcacg
     6361 aggctcttga gcaagcggcc cgacgtgggg ccttgcagcg cgagcgccgc cgtctcctcg
     6421 gagatgtcct cgatctccac gtccatcccc gcggcgttca tcgtgaacca gcgcagggaa
     6481 gggtcggcgg ccgtccagcg gtagagattc tcgccgaggc gcgagaccgt gccgtcgtcg
     6541 atcaccttcc cgcgctcgtc gcaccacggc gtatacacga cctgcccgac cgagacctta
     6601 cgcatgtcgc gcgtgatgat tctatcgacg agccgggtag cgtcgcggcc cgtgagccgg
     6661 tacttgtaga gcggcgagat gtcgatgagc gcggcggcgt tccgtatcgc gttgtactcg
     6721 tgctcgtggt gcggctcgta gctgctcacc gcgtaatagc ccgaccactc gcggtagctc
     6781 aggctctcgc agagcgcgaa agttctttcg tgaaaagcgg ttccgattgg catgtaagat
     6841 tctcagtgtg cgattgtcac ggcgagcgat tataagtatc atcgcaagtc ttccgtcaag
     6901 aactaactaa cacatggcac aaaagtacga cgttatcgtc atcggcggcg ggcacaacgg
     6961 gctggtgtgt gccgcgtatc tggcgcgcac gggcaagaag gttctggtgc tggagcggcg
     7021 gcacgttctg ggcggcgcgg ccgtcacgga agaggtgttt ccgggcttca agttctcggt
     7081 cgcctcctac gtggtgtcgc tgctgcgccc cgaaatcatc cgcgagcttg atttgccgcg
     7141 gcacggtctg gaaatcctcc cgctcgacgg gacgtttacg ccgatgctga acggcgacta
     7201 cctgtggcgc acgaacgacc acgcgaagac acggcgcgag atcgcgcgcc actcgaagct
     7261 cgacgcc
//
LOCUS       DASVES010000159         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_605432, whole genome shotgun sequence.
ACCESSION   DASVES010000159 DASVES010000000
VERSION     DASVES010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605432"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VF240_06425"
     CDS             <1..1353
                     /locus_tag="VF240_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7174898.1"
                     /translation="AVLQPNFRGSTGYGKKFLLAANNEWGQKMQDDITWGVKHLVAEG
                     VADPKRVGIMGGSYGGYATLAGLAFTPDVYAAGVSIVGPSNLITLLNSIPPYWESIRK
                     LFHERMGDPSTPAGKAMLERQSPLGSADKIKSPLLVVQGANDPRVKKAESDQIVVALR
                     ERGFPVEYINAPDEGHGFARPVNNMAMFAAAEKFLAKHLGGRFQESMTPETATRLKEI
                     TVDVKTVEMPKRIEATAVGLPKPAFDLQPGTHTYKGTLAAGGQSMPLPVTVEVKEEGG
                     SWIVSETAVLQGTTISDITTLEKGTLVVKHRSIKQGPVAIELDFKDNKATGTMSMGGG
                     PPKPISVDLGGALFADGAGSHDVLAALPLAPGYVTTFRNLDVQRQQVQLKQLKVVGSE
                     SVTVPAGAFDAHKLELTDADGSGGATHIWVAKDTRKVVKVSATLPQMGGAVITTELEK
                     "
     gene            1433..1633
                     /locus_tag="VF240_06430"
     CDS             1433..1633
                     /locus_tag="VF240_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174899.1"
                     /translation="MSVTTFEGLVEQGQIRLASDVRLPEHTKVYIIVPDIELKEKSLT
                     LQSPRLAQPERAAEFEMEVVEL"
     gene            1638..2024
                     /locus_tag="VF240_06435"
     CDS             1638..2024
                     /locus_tag="VF240_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174900.1"
                     /translation="MPTYDSHRFNPSAPVALVSLRNPESGATKADVPMLLDTGADVTL
                     LPGSIITGLGVALLPNKSYELLGFEGQESMAHAVRAEMLFLGLTFRGQFLIVEQEWGI
                     IGRNVLNALSLTLDGPNLRWDRTPAA"
     gene            2152..2604
                     /locus_tag="VF240_06440"
     CDS             2152..2604
                     /locus_tag="VF240_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174901.1"
                     /translation="MDEIAKKMAEVEWVYADKDPDALSALEERIAETGRKLGLITYSE
                     LVKGVDFHLPNIRKGEGYRIQIYDWSGLDRAILGEFLGYISTRSYREAGFMASALVVN
                     STGLEPSEQFFEWMERLGVLPDTNKDTVLAFWAEQVHKAHNWFRAGRR"
     gene            2616..>3902
                     /gene="hydA"
                     /locus_tag="VF240_06445"
     CDS             2616..>3902
                     /gene="hydA"
                     /locus_tag="VF240_06445"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX7174902.1"
                     /translation="MNTLIRNGRVVTAVDDYKADILIEGETVSVIGAKLDMEADRVID
                     AAGKLVIPGGIDPHTHMELPFGGTQASDDFRTGTVAAAHGGTTTIIDFAVQFKGQSLI
                     EAVDNWHAKAEGKCAIDYSFHLITTELEDNRIEELHTVMDEGVTSFKMFMAYPGVFLV
                     DDATIFRAMSAAGERGGLICMHAENGIVINEIIKRALAQGRTAPKYHALTRPTRAEAE
                     GVHRAIAIAEMAESPVYIVHLSCADALNQVREARDRGLPAFAETCPQYLFLSIDDYGE
                     GFEGAKYVMTPPLREKWNQDELWKGLKMDDLQVISTDHCPFCMKEQKELGRDDFTKIP
                     NGAPGVEHRVPLIYDGGVAQGRISLNRFVELTSTAAAKMFGLFPRKGTIAVGSDADIL
                     IFDPEREQTISARTHHMNVDYSAYEGRTIKGVVETVL"
BASE COUNT          814 a         1211 c         1192 g          685 t
ORIGIN      
        1 gccgtgctcc agccaaactt ccgcggctcc accggctacg gcaaaaagtt tctgctcgcc
       61 gccaacaacg agtggggcca gaagatgcag gacgacatca cgtggggcgt caagcacctc
      121 gtcgctgaag gcgtcgctga cccgaagcgc gtcggcatca tgggcggctc ctacggcggc
      181 tacgccacgc tcgccggtct cgccttcacg cccgacgtgt acgccgccgg cgtctccatc
      241 gtcggcccgt cgaacctcat cacgctcctc aactcgatac cgccctactg ggagtcgatt
      301 cgcaaactct tccacgagcg catgggcgat ccctcgacgc ccgccggcaa ggcgatgctc
      361 gaacgacagt cgccgctcgg ctcggccgac aagatcaagt cgccgctctt ggtcgttcag
      421 ggggcgaacg accctcgcgt gaagaaggcc gagtcggatc agatcgtcgt cgcgctgcgc
      481 gagcgcggct tccccgtcga gtacatcaac gcgcctgacg agggccacgg cttcgcgcgc
      541 cccgtcaaca acatggcgat gttcgcggcg gccgagaagt ttctcgccaa gcatctcggc
      601 gggcgctttc aggagtcgat gacgccggag acggccacgc gtctcaagga gatcacggtt
      661 gacgtgaaga cggtcgagat gccgaagcgc atcgaggcga ctgccgtcgg actcccgaag
      721 ccagcctttg acctccaacc cggcacgcac acttacaagg gcacgcttgc agccggcggc
      781 cagtcgatgc cgctgcccgt cacagtcgaa gtgaaggagg agggcggctc gtggatcgtc
      841 agcgagacgg ccgtcttgca gggcacgacg atcagcgaca tcacgacgct tgagaagggc
      901 acgctcgtcg tcaagcatcg ctcgatcaaa caggggccgg tcgccatcga actcgacttc
      961 aaggacaaca aggccaccgg cacgatgagc atgggcggcg gcccgcccaa gcccatctcg
     1021 gttgacctcg gcggcgccct cttcgccgac ggcgccggct cgcacgacgt gctcgccgcc
     1081 ctgccgctcg cgcccggcta cgtcaccacc ttccgcaacc tcgacgtgca gcgccagcag
     1141 gtgcagctca agcagttgaa ggtcgtcggc tcggaatccg tcaccgtccc ggccggcgcc
     1201 ttcgacgcac acaagctcga actcaccgac gccgacggca gcggcggcgc cacacacatc
     1261 tgggtggcga aagacacacg caaagtcgtc aaggtcagcg ccactctccc gcagatgggc
     1321 ggcgcggtca tcacgacgga gcttgagaag taacctcccg cgctgagtgg gccggcaacg
     1381 ctcccttgca tcgccggccc acttattaat tttttagcgg cggtgcaaaa ccatgagcgt
     1441 gaccaccttt gaaggactcg tggaacaggg gcagatcagg ctggcctcgg acgttcgcct
     1501 acccgaacac acgaaggtct acatcatcgt ccccgacatt gagttaaaag aaaaaagcct
     1561 gaccctccaa agcccgcgtc tggctcaacc tgagcgggcg gcggaattcg agatggaggt
     1621 cgtcgagctg tagcgggatg ccgacatacg attcgcacag gttcaacccg tccgccccgg
     1681 tcgcactcgt ttccctccgt aacccggaga gcggcgcgac gaaagcggac gtgccgatgc
     1741 tccttgatac cggcgcggac gtgactctgt tgccgggaag tattatcaca ggactaggcg
     1801 tggctcttct cccaaacaag agttacgagc tgctcggctt tgaaggccag gagagtatgg
     1861 cccacgcggt tcgagccgag atgcttttcc tcggcctgac ttttcgcggc cagtttttaa
     1921 tcgtcgaaca agagtggggc atcatcggac gcaacgtcct gaacgcactt tcattaacgc
     1981 tcgacgggcc aaatctaagg tgggacagga cgcctgccgc ctgaacaggg gtcgcgagta
     2041 gggagacaga tgaagacatt gattcgcaac ggccgcgtcg tcacggcggc agatgactac
     2101 gaggccaacc gcttcatgtc tgtttagctt gcggcaatta tggagaaacc catggatgag
     2161 attgctaaga agatggctga ggtggagtgg gtttacgccg ataaagaccc agacgcatta
     2221 tctgcccttg aagagcggat tgccgaaaca ggtcgaaaat taggacttat cacttactct
     2281 gagctggtga aaggtgtgga cttccacttg cctaatatcc gcaaaggcga gggatacaga
     2341 attcaaattt atgactggtc aggactagat agggccatac ttggtgaatt cttgggctac
     2401 atttccactc gcagttatcg cgaagcaggg tttatggcta gtgctcttgt cgttaatagt
     2461 actggtttag agcccagtga gcagtttttt gaatggatgg aaagactcgg agtattgcct
     2521 gatacaaata aggatactgt cctagctttc tgggctgagc aggttcataa agctcataac
     2581 tggtttagag caggtcgccg ttaaaggaag gtctaatgaa cacgctcatt cgcaacggcc
     2641 gcgtcgtcac ggcggtcgat gattataagg ccgacattct catcgaaggc gagactgtct
     2701 ccgtcatcgg cgcgaagctg gacatggagg ccgaccgggt gattgatgcg gcggggaagc
     2761 tcgtcatacc gggcggcatc gacccgcaca cgcacatgga gctgccgttc ggcgggacgc
     2821 aggcgtcaga cgactttcgc accggcactg tggcggcggc gcacggcggg acgacgacga
     2881 ttatcgactt cgccgtgcag ttcaaagggc agtcgctcat cgaggccgtg gacaactggc
     2941 acgcgaaggc cgagggcaag tgcgcgattg attacagctt ccacctcatc acgaccgaac
     3001 tggaagacaa ccgcatcgaa gagctgcaca cggtgatgga cgagggcgtg acgagcttca
     3061 agatgttcat ggcctacccc ggcgtcttcc tcgttgacga cgcgaccatc ttccgcgcga
     3121 tgtcggccgc gggcgagcgc ggcggcctca tctgcatgca cgccgagaac ggcatcgtca
     3181 tcaacgagat catcaagcgc gctctggcgc aggggcgcac cgcgccgaag tatcacgcgc
     3241 tgacgcggcc gacgcgcgcc gaggccgagg gcgtccaccg cgcgattgcg attgccgaga
     3301 tggccgagtc gcccgtctac atcgtccacc tcagttgcgc cgacgccttg aatcaggtgc
     3361 gcgaggcgcg cgaccgcgga ctgccggcct tcgccgagac gtgcccgcag tatctcttcc
     3421 tctcgattga cgattacggc gagggcttcg agggcgcgaa gtatgtgatg acgcccccct
     3481 tgcgtgagaa gtggaatcag gatgagcttt ggaagggtct gaagatggac gacctgcaag
     3541 tgatctcgac cgaccactgc ccgttctgca tgaaggagca gaaggagctg gggcgcgacg
     3601 acttcacgaa gattcccaac ggcgcgcccg gcgtcgaaca ccgcgtgccg ctcatctacg
     3661 acggcggcgt cgcgcagggc cgcatctcgc tcaaccgctt cgtcgagctg acctcaacgg
     3721 ccgccgcgaa gatgttcggc ctcttcccgc gcaaaggcac catcgccgtc ggctccgacg
     3781 ccgacatcct catcttcgac cccgagcgcg agcagaccat cagcgcccgc acgcaccaca
     3841 tgaacgtcga ttacagcgcc tacgagggcc ggacaatcaa gggcgtggtc gagacggtgc
     3901 tc
//
LOCUS       DASVES010000160        12694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1381528, whole genome shotgun sequence.
ACCESSION   DASVES010000160 DASVES010000000
VERSION     DASVES010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12694
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381528"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..684)
                     /locus_tag="VF240_06450"
     CDS             complement(76..684)
                     /locus_tag="VF240_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174903.1"
                     /translation="MRKLYNSALAAAFILSLAGGAAAQRTATVTEPQPAAAPTTPGQS
                     VTTPKPPPAPASVKTKYEGGVVGYGKAEGTLNFDDVNRRLVFRDKKQGKEVFAVPYDV
                     VHAAWTDSKSQRSTAGTVVSHIPYGGIPGMFMKSKTRYLILQYRDPETAASGLASFKF
                     DNKELLASVLHTFGQKAGLTQRGEAFIRRTVPAAATNTTNNP"
     gene            complement(737..1351)
                     /locus_tag="VF240_06455"
     CDS             complement(737..1351)
                     /locus_tag="VF240_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174904.1"
                     /translation="MTRIARIVKSNSHVDYVGRVIDPLDAAEPPTADDYGFAQFVSMP
                     LEGAGGEVVGVVYDTQLVNPDYGSLGPRLSPREEIAVLSPDYLNEQGVLVGILLLGWR
                     EAGGAEGRGEYRHGVPRRVIPVGQDVYAMADEGVREFHRGEDGSVGLRYFSQVVAHAG
                     MFALPVVEAVISQLERDCTAQDRQRLCVLKRSLSWQRTLGQMRL"
     gene            complement(1482..2006)
                     /locus_tag="VF240_06460"
     CDS             complement(1482..2006)
                     /locus_tag="VF240_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174905.1"
                     /translation="MNTGAEREPDAGREAEAMCPACGASQRRTEARFCATCGRELRGG
                     ADYFPLDSLRASYHLQDRRGGGRVSQLVARASRPVVASSPHPRAMPLDRNSNGAATTA
                     LAFVTYSLVPYLGILFCPGALVMGGVGLLRAQRAPHVGGRRAAATSIVLGVVVFGAQI
                     FLWWILYKIPEWTR"
     gene            complement(2227..3450)
                     /locus_tag="VF240_06465"
     CDS             complement(2227..3450)
                     /locus_tag="VF240_06465"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX7174906.1"
                     /translation="MLYRTRLITELHSRREEFVSFGQTLRGEVREASARLRALAAEGS
                     VAVRERVEAAGAERVSYPSAEMDVANDVAVAFGPRWRSHEEARAWAAATLRGRTTFAA
                     DGSQIYPGREVSLPVAAVQIASFENPHTPDGAYTKEAHFKIITPGELQESGRVYDSPE
                     QVVNSRRFELEAAALRDFVERRRGWQERGERAPVAFLDGTLLFSPFRKGSEDTFSKRQ
                     AAAAAGLVAASREARVPVVGYVDHSYAPDLCRALEAFHPDAAQTGAYDAQILRAPARD
                     GGVAPLLAAWGDRTVFWHCRRPNLSADFYDERGNPLVGFVYLQTTAGGNPARVEIPAW
                     VYEAGLLEDVVDAVRAECVVGNGYPYAVETADAAAAMTTRDREQFLRVMQDFADEHRF
                     DFRISRKPMSKSRRR"
     gene            complement(3541..4059)
                     /locus_tag="VF240_06470"
     CDS             complement(3541..4059)
                     /locus_tag="VF240_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174907.1"
                     /translation="MSNHKSLYASARGGKAALVALTFAALFVANVHVAGAASWKGIEP
                     LKSRRADVERVLGMAARETMADGALHFNVAGGRVTIFFLSAKTVAVKKLSPALEGTVL
                     QIVLQHDTASDTPESLGLDKGKRFEREAKGDVLFYNNQRDGVAYIFIGGKLRTTRYSA
                     PAEEFAKTLKRK"
     gene            complement(4418..5650)
                     /locus_tag="VF240_06475"
     CDS             complement(4418..5650)
                     /locus_tag="VF240_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7174908.1"
                     /translation="MSKSKISTAAVIAALVCCATAADAGAQSGATRPRRVNPSQPVAT
                     SPAPRASDEPAASAPATTRPATPAARTAAPASDTARAFALFQQKQFDAALAEARKATS
                     ADPKNSEGWKIAGFAEMELKRYADAATDLLRALELQRAEGAEDANTSDALATAYVRTE
                     KYAEALPLLVAATTRKGTRPDAITFYYRGLSEFRTNKLAESERSFNEAVKLNPKDAAS
                     LYYLGRLAFDRKDNDAAVNLLNRATAANAQFAEAWTYLAHAYLRRGAASGEGPKADAD
                     YQGAVRASEALMRLRSDEPAVLLHGQALINSKQYARAATVLERAATGDAAKGETLYLL
                     GFAYTQAKNHPKAVAALERAAAKTPEDANVYRFLGYNYQLLKQYAKALAAYERGMALA
                     PDDAYFKEAADQVRPFAK"
     gene            complement(6028..6318)
                     /locus_tag="VF240_06480"
     CDS             complement(6028..6318)
                     /locus_tag="VF240_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174909.1"
                     /translation="MMYAFWALVGWCGTPPGKGPFKPDPDPEPWWMSRVAALVGGILG
                     GILVRRMVNGADTDAAVIIATAIGAYAVGRVLGDIVSNVVGGNRGNRVNVGP"
     gene            complement(6546..6752)
                     /locus_tag="VF240_06485"
     CDS             complement(6546..6752)
                     /locus_tag="VF240_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174910.1"
                     /translation="MRTAGLIVLASTLFLVSLLLLVWFSGFITGIAGSLVHLLLVIAL
                     LIAPVGTVVGVALLVMASRQSPPR"
     gene            complement(6775..7473)
                     /gene="purQ"
                     /locus_tag="VF240_06490"
     CDS             complement(6775..7473)
                     /gene="purQ"
                     /locus_tag="VF240_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX7174911.1"
                     /translation="MKFGVIVFPGSNCDHDAYHVISKHVGQPVDFVWHRDTDLSPYDA
                     VIIPGGFSYGDYLRAGALARFSPVMASVKEFATRGGLVLGICNGFQILCEAGLLPGAL
                     IRNKELHFICQHVQVRVETTETPFTHMLEAGGVLRLPIAHAEGNYVCDDETFDELVGE
                     DRVIFRYCDAAGELKGEANLNGSRDHIAGICSPGRNVLGMMPHPERACEDLLGSSDGR
                     DLFRSLAATLAAHA"
     gene            complement(7470..7715)
                     /gene="purS"
                     /locus_tag="VF240_06495"
     CDS             complement(7470..7715)
                     /gene="purS"
                     /locus_tag="VF240_06495"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX7174912.1"
                     /translation="MRAKVYVTLKPGVLDPQGKAIQHSVGLLGFDRIRDVRQGKYFEI
                     ALDDGVDEAAARAEAERMARDVLSNPVIEDFRVEIER"
     gene            complement(7865..9550)
                     /locus_tag="VF240_06500"
     CDS             complement(7865..9550)
                     /locus_tag="VF240_06500"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX7174913.1"
                     /translation="MKRLITLVAVVLLLSSPLRAQGARGEFDVIVRGGTVYDGTGRAP
                     RRADVGIRGDRVAVVGNLSRERAKTIVDARGAAVAPGFINMLSWSTDSLIADARSQGE
                     IRQGVTTQIFGEGWSMGPLSDAMKRQMVADQGDIKFAVEWTTLAEYLRHLEARGVSQN
                     VASFVGATTIRIHVLGERDVRPTPAQLEEMKELVRREMEAGALGVGTSLIYPPAFYAS
                     TEELIELCKVAARYRGKYISHMRSEGARLIEAVEELIRISREAGIPAEIYHLKAAGRD
                     NWPKMARVLRMVNDARRQGLKITADMYTYPAGATGLTAALPPWSQDGGFEELVRRIKD
                     AETRRRILEEMRTPTDKWENLYLAAGSAERVLLSGFKTEALKQLTGKTLASVAKERGT
                     SAEDTILDLIVEDGTRVEAVYFLMSEENIRAQLRQPWVSFGSDASSMAPEGVFLKSST
                     HPRAYGNFARLLGKYVREERVITLTEAVRRLTSLPATNLGLTGRGLLRPGMFADVVVF
                     DPAKVADRATFDRPHQYSVGVRHVLVNGVPVLRDGEHTGATPGRALWGPGKVK"
     gene            complement(9791..11128)
                     /gene="purB"
                     /locus_tag="VF240_06505"
     CDS             complement(9791..11128)
                     /gene="purB"
                     /locus_tag="VF240_06505"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7174914.1"
                     /translation="MIPRYTLPEMGALWSEQNKFQKWLDVELAVCEVHAEMGTIPREA
                     VGEIKKKATFTVERINEIEKTTDHDVIAFTTALAESIGEAARFVHYGLTSSDVVDTAN
                     ALLLRDACDLLLGRTDALMEVLKRRAYEFRRTPQVGRTHGVHAEPTSFGLTWALWYDE
                     MRRNHARLVRAREAVAVGKISGAVGAFAHLDPAVEEGVCARLGLRAAPVSTQIIQRDV
                     YAEYLTTAAIVASSLEKFALQVRHWQRTEVREAEERFRAGQKGSSAMPHKRNPILSER
                     ICGMARVMRGYAVAGLESNALWHERDISHSSAERVVLPDASIALDYLLAKTTSLLDTL
                     VVHPERMLENLDATRGLVFSGQLLLSLTRAGVSREDAYLWAQRNAMRAWDEGEDFRAL
                     VLEDADINSKLPRERIESVFSPDTYLRNVDAIFARVFGEDHSAADASTEVGIL"
     gene            complement(11214..11612)
                     /locus_tag="VF240_06510"
     CDS             complement(11214..11612)
                     /locus_tag="VF240_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HEX7174915.1"
                     /translation="MPFAFLACEVDERRQVTPEQQAAIDRVTEEIAAARDRELYDGAA
                     DEWRAVVTAEESRDNLERVRTRLGRVQSRALHTGTEKETAAGPLTGHSLVVTYQTTFE
                     RGTGMETFTLLERDGRWLLAGYTVTSDALK"
     gene            complement(11693..12520)
                     /locus_tag="VF240_06515"
     CDS             complement(11693..12520)
                     /locus_tag="VF240_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7174916.1"
                     /translation="MRFTVLGSGSTGNAVLICAGRTRVLVDAGLSAKELARRLALVGE
                     DVQALDGVIVTHEHGDHAGGLRVLLKSLSCPVYISGETRDAYIREHAGSSAKDEPRKR
                     AAAVRDRTEEISSSKDFRIGEIDFHPFTIPHDAADNFGFVASSGGVRVATLMDFGHVT
                     TLVAERLKACAAIVIESNHSRDMLKACEVYPWELKQRILSRTGHLSNEDVAVWLRDDF
                     DGAAEHVVLAHLSQRANNPYVAKLSAEIALQERGRLFQTTTKITLSHPKEPTPWIEI"
BASE COUNT         2074 a         3990 c         4431 g         2199 t
ORIGIN      
        1 gtaagacgaa gcggcgcgac gcggttcgag ttgaaccgcg ccgcgccgct ttggtgttcc
       61 gggtcggatc ggtgtttagg gattgttggt ggtgttcgtc gcggcggccg gcacggtgcg
      121 gcggatgaag gcttcgccgc gctgggtgag tccggccttc tggccgaagg tgtggaggac
      181 ggaggcgagc aactccttgt tgtcgaactt gaagctggcg aggccggagg cggcagtttc
      241 cgggtcgcgg tactggagga tgaggtagcg cgtcttgctc ttcatgaaca tccccggaat
      301 cccgccgtag gggatgtggg agacgaccgt gccggccgtc gagcgctgcg acttcgagtc
      361 ggtccaggcg gcgtggacga catcgtaggg gacggcgaag acctctttgc cctgcttctt
      421 gtcgcggaag acgaggcggc ggttcacgtc gtcgaagttg agcgtgccct cggccttgcc
      481 gtagccgacg accccgccct cgtacttcgt cttgaccgag gcgggcgccg ggggcggctt
      541 cggcgtcgtc actgactggc ccggcgtcgt gggcgccgcg gcgggctggg gctccgtgac
      601 ggtggccgtg cgctgtgcgg cggccccgcc ggcgagcgag aggatgaagg ccgcggccag
      661 cgcggagttg tagagctttc tcatttctaa ttgcctttct gtattgctga tagaggttgt
      721 tcggaattgt ccgacattaa agacgcattt ggccgagcgt tcgttgccac gagagtgagc
      781 gtttgagcac gcacaggcgt tgccgatcct gcgctgtgca gtcgcgctcc agttgagaga
      841 tgacggcctc gaccacgggc agggcgaaca tgccggcgtg cgccacgacc tgcgagaagt
      901 agcgcaggcc caccgagccg tcctccccgc ggtgaaattc gcgcaccccc tcgtccgcca
      961 tcgcgtacac gtcctgcccg acggggatga cgcggcgcgg cacgccgtgt cgatactcgc
     1021 ccctcccctc cgcgccgccc gcctcgcgcc agccgagcag aaggatgccg acgagcacgc
     1081 cctgctcgtt caggtagtcg gggcttaaga cggcgatctc ttcgcgcggc gaaagacgcg
     1141 ggccgaggct cccgtaatcc gggttgacga gctgcgtgtc gtagacgacg ccgaccacct
     1201 cgccgcccgc cccttcgagc ggcatcgaga cgaactgcgc gaacccgtaa tcgtcggccg
     1261 tcggcggctc cgcggcgtcc agcgggtcga tcacccgtcc gacgtaatcg acgtgcgagt
     1321 tcgacttgac gatcctggcg atgcgagtca tgggaagtcg taaatcgtga atcgtgaatc
     1381 gtgaatcgtg aatgagcggc catcctgccg gtgtagtcaa tcgcgagtcg tatcagcgtc
     1441 aatcccatcg atttacgatt cacgatttac gtctttattc cctatcgcgt ccactccggg
     1501 attttgtaga gaatccacca cagaaaaatc tgcgcgccga agacgaccac gccgagcacg
     1561 atgctcgtgg ccgcggcgcg gcggcccccg acgtgcggcg cgcgctgggc gcgcaggagt
     1621 ccgacgccgc ccatcacgag cgcgccgggg cagaagagga tgccgaggta ggggacgagt
     1681 gagtaggtga cgaaggcgag cgcggtggtc gccgcgccgt tcgagtttct gtctaagggc
     1741 atggctctcg ggtgcggcga cgacgcgacg acggggcgcg acgcgcgcgc gacgagttgg
     1801 ctgacgcggc ccccgccgcg tcgatcctgc aagtgatagg aggcgcgcag ggagtcgagc
     1861 gggaagtagt cggcgccgcc gcgcagctcg cgcccgcacg tcgcgcagaa gcgcgcctcg
     1921 gtgcggcgtt ggctggctcc gcacgcgggg cacatggcct cggcctcacg ccccgcatcc
     1981 ggctctctct cggcgccagt gttcataaaa cgtaacgcat cccacgccct cgcggcagac
     2041 actttcgcat gcgctcccct cgcgcttcaa aaataccccc tcggcacgaa ggacgcaaag
     2101 agtgttgagc gtgcgcctca gtaccgcgag cggaagcctc gcgggtcagc cgcgcgcgtt
     2161 gcggtctata ggtttaacgc atacccgctc gctaccgctc gcggtactgt ttaagcggcg
     2221 cggcactcag cgacggcgcg acttgctcat gggcttacgc gagatgcgga agtcgaagcg
     2281 gtgctcgtcg gcgaagtcct gcatgacgcg caggaactgc tcgcggtcgc gcgtcgtcat
     2341 ggcggcggcc gcgtcggccg tctcgaccgc gtaggggtag ccgttgccga ccacacactc
     2401 ggcgcgcacc gcgtccacga catcttcgag caggcccgcc tcgtagaccc acgccggaat
     2461 ctcgacgcgg gccgggttgc cgccggccgt cgtctgtagg tagacgaaac cgacaagcgg
     2521 gttgccccgc tcgtcgtaga agtccgcgga gaggttcggg cggcggcagt gccagaagac
     2581 ggtgcggtcg ccccaagcgg caagtagtgg cgcgacgccg ccatcgcgcg ccggggcgcg
     2641 gagaatttgc gcgtcgtagg cgccggtctg cgccgcgtcg gggtggaagg cttcgagcgc
     2701 gcggcagagg tcgggcgcgt aggaatgatc gacgtagccg acgaccggga cacgggcctc
     2761 gcgcgaggcg gcgacgagac cggccgcggc cgcggcctgc cgcttcgaga aggtgtcctc
     2821 ggagcctttg cggaaggggg agaacaaaag cgtgccgtcg aggaaggcga cgggcgcacg
     2881 ctcgccgcgc tcctgccagc cgcgccggcg ctcgacgaag tcgcgcagcg cggcggcctc
     2941 cagctcgaag cgccgcgagt tgacgacctg ctcggggctg tcgtagacgc gcccgctctc
     3001 ctgcaattcg ccgggcgtga tgatcttgaa gtgcgcttcc ttcgtgtaag cgccgtcggg
     3061 cgtgtgtggg ttctcgaagg aggcgatctg gacggcggcc accggcagag agacctcgcg
     3121 gccgggataa atctggctcc cgtcggccgc aaacgtcgtg cggccccgga gcgtcgcggc
     3181 ggcccacgcg cgcgcctcct cgtgcgagcg ccagcgcggg ccgaacgcga cggccacatc
     3241 attcgccacg tccatctccg ccgacgggta actcacgcgc tcagcgcccg ccgcctccac
     3301 gcgctcgcgc accgccacag acccttcggc cgccagagcg cgcagacgcg ccgacgcctc
     3361 gcgcacttcc ccgcgcagcg tctgcccgaa gctcacgaac tcctcgcgcc gcgagtgcag
     3421 ctcagtgatt aatcgtgtgc ggtagagcat gagggcaaaa aggcaagagg tcagaggtta
     3481 gaggttagag gtcagtaaag acagcttttc actgacctct aacctctgac ctcttgtctt
     3541 tcattttcgc ttgagagtct tggcaaactc ctcggccggt gcggagtagc gcgtggtgcg
     3601 gagcttgccg ccgatgaaga tgtaggcgac gccgtcgcgc tggttgttgt agaagagcac
     3661 gtcgcccttc gcctcgcgct cgaaccgctt gcccttgtcg agaccgagcg actcgggcgt
     3721 gtcggaggcg gtgtcgtgtt ggaggacgat ctgaaggacg gtgccttcga gtgcgggcga
     3781 gagctttttg acggcgacgg tcttggcgga gaggaagaag atcgtcacgc gcccgccggc
     3841 cacgttgaag tggagcgccc cgtccgccat cgtctcgcgc gcggccatgc cgagcacgcg
     3901 ctccacgtcg gcgcgccgcg acttcagcgg ctcgatgccc ttccacgagg cggccccggc
     3961 gacgtggacg ttggcgacga agagcgcagc gaaggtcaac gcgacgagcg cggctttccc
     4021 gccgcgcgca gatgcgtaga gagatttgtg gttactcatg gcgtgatgct acagcccaga
     4081 attttaaagt tcaagaccca aagctcaacg gacgctcgac ggcgaaatac tacgagcttt
     4141 cccgaacgac aggactggac gagacgcgcg ccgctagccg ctgaaagcgg cggaagaatg
     4201 tagcccacgg cgtaagccgt ggggacaagg ccgggaaagc aatcgaagcc ccggaggggc
     4261 gaaagaatct cgacgggcaa cacgctcaac gttgagattc ttccgcccct ccggggcttc
     4321 tcaatctttg gtgcgaacgt cccacggctt acgccgtggg ctacattctg ccgccgcctc
     4381 cggcggctca ttgtctctta gttgacgaac ttgcgtctta cttcgcgaag gggcggactt
     4441 gatcggccgc ctccttgaag taggcgtcgt cgggcgcgag cgccatgccg cgctcgtagg
     4501 cggcgagggc cttggcgtat tgcttgagca gttgatagtt gtagccgagg aagcggtaga
     4561 cgttggcgtc ttcgggcgtc ttcgcggcgg cgcgctctaa ggcggcgacg gccttcgggt
     4621 ggttcttggc ctgcgtgtag gcgaagccga ggaggtagag cgtctcgccc ttggcggcgt
     4681 cgcccgtcgc cgcgcgttcg agcaccgtcg cggcgcgcgc gtactgcttc gagttgatga
     4741 gcgcctgacc gtggagcagg acggccggct cgtccgagcg caggcgcatc agggcctcgc
     4801 tggcgcgcac ggccccctga tagtcggcgt ccgccttcgg cccttcgccg gaggccgcgc
     4861 cgcggcgcag gtaagcgtgg gccagatacg tccacgcctc ggcgaactgg gcgttggcgg
     4921 ccgtggcgcg gttgaggaga ttgacggccg cgtcgttgtc cttgcggtcg aaggcgaggc
     4981 ggccgaggta atagagcgag gccgcgtcct tcggattgag tttgacggcc tcgttgaagg
     5041 agcgctcgga ctcggcgagc ttgttcgtgc ggaactcgga gaggccgcgg tagtagaagg
     5101 tgatcgcgtc ggggcgcgtg cccttgcgcg tcgtcgcggc gacgaggagc gggagcgcct
     5161 ccgcatattt ctccgtgcgg acgtaggcgg tggcgagcgc gtccgacgtg ttcgcgtcct
     5221 cggccccctc ggcgcgctgg agttcgagtg cgcgcaggag gtctgtggcg gcgtcggcgt
     5281 agcgcttcag ctccatctcg gcgaagccgg cgatcttcca gccttcggag ttcttcgggt
     5341 cggcggaggt cgccttgcgc gcttcggcga gggccgcgtc gaactgcttc tgctggaaga
     5401 gcgcgaaggc gcgcgcggtg tcggaggcgg gcgcggcggt tctggcggcg ggggtcgcgg
     5461 gccgcgtcgt ggccggggcc gacgcggcag gctcgtccga cgcgcgcggc gcgggcgagg
     5521 tggcgaccgg ctgcgacggg ttgacccggc gcggacgcgt cgcgcccgac tgcgctccgg
     5581 cgtcggcggc ggtggcgcag cagacgagtg cggcgataac ggcggcggtg ctgatcttcg
     5641 atttcgacat ggcttgaaag tcttccaagt gcccggcggg ggtttcgcaa cccggcggcg
     5701 tcctttcggt tcgggtaagg gagagtggcg tgtgtccgat gcgacaaagg gggcgcgcgg
     5761 ttcgccgcga aagattgtaa ctcaagggga gggcggtgga acacggatga aacggattgc
     5821 ggacggatga gcacggattt aatccgtgtg aatctgtctc aatccgtctg attcgtgttc
     5881 cccactaccc gcctcgcaag ccgcacacaa atgcagactg ctaacacgac gaaaggcgga
     5941 gggcggaagc gatgggcgca cacgccgcgc gcccatcgct tccgccctcc gcctccggcc
     6001 ttctcgtcgg acgcggggtg tcagacccta cgggccgacg ttgaccctgt tgcccctgtt
     6061 gccgcccacg acgttggaga cgatgtcgcc gaggacgcgg ccgacggcgt aagcgccgat
     6121 ggccgtggcg atgatgacag ccgcgtccgt gtcagcgccg ttcaccatgc gccggacgag
     6181 gatgccgccg aggatgccgc ccacgagggc ggcgacccta ctcatccacc acggctcggg
     6241 gtcggggtcg ggtttgaaag gccccttccc cggcggggtg ccacaccagc ctaccagtgc
     6301 ccagaatgca tacatcatca gcgtctacct ccttagtggg gttaaaaagt cggttggcag
     6361 ttggagcgcc gattccaagc tctcgaatct atgccccgtt tgttgatgcg tgtgtgagac
     6421 cggcggcagg agcgggcccg cgacggcgta gaaccccagg taggaacgcc ggcacgatat
     6481 gcccgcacgg gtgaaatgtc aacgattttc ggccccacgg gcgtgtgtgc gcgcgcggcc
     6541 ccgcgctagc gcggaggcga ctgtctggag gccatgacga ggagcgccac gccgacgacc
     6601 gtgccgacgg gcgcgatgag gagcgcgatg acgagcagca ggtggacgag gctgccggcg
     6661 attccggtga tgaacccgct gaaccagacg agcagcagga gactgaccag aaagagcgtc
     6721 gaggcgagga cgatgagacc ggctgtacgc acgtcgatga aaactcctct cgtttcaggc
     6781 gtgcgcggcg agcgtggcgg cgagcgagcg gaagaggtcg cggccgtcgg acgagccgag
     6841 caggtcttcg caggcgcgct cggggtgcgg catcatcccg agcacgttgc gccccggcga
     6901 gcagatgccg gcgatgtggt cgcgcgagcc gttcaggttg gcctcgccct tgagttcacc
     6961 cgcggcgtcg cagtagcgga agatgacgcg gtcttcgccg acgagttcgt cgaacgtctc
     7021 gtcgtcgcag acgtagttgc cctcggcgtg ggcgatgggc aggcgcaaga cgcctcccgc
     7081 ttcgagcatg tgcgtgaagg gcgtctcggt cgtctcgacg cgaacctgca cgtgctggca
     7141 gatgaagtgc agctccttgt tgcggatgag cgcgcccggc agcaggcccg cctcgcagag
     7201 aatctggaag ccgttgcaga tgcccaggac gaggccgccc cgcgtggcga actccttgac
     7261 ggaggccatg acgggcgaga agcgggcgag cgcgccggcc cgcaggtagt cgccgtagga
     7321 gaagccgccg gggatgatga cggcgtcgta aggcgagagg tcggtgtcgc ggtgccagac
     7381 gaaatcgacg ggctggccga cgtgcttcga gatgacgtga taagcgtcgt ggtcgcagtt
     7441 cgagccgggg aagacgatca caccgaattt catcgctcaa tctccacgcg gaagtcttcg
     7501 atgacggggt tcgagagcac gtcgcgcgcc atgcgctcgg cctcggcgcg cgcggccgct
     7561 tcgtccacgc cgtcgtccag agcaatctcg aaatacttgc cctgacgcac gtcgcggatg
     7621 cggtcgaagc ccaaaagccc gaccgagtgt tgaatggcct tgccctgcgg gtcgagcacg
     7681 cccggcttga gtgtgacgta gactttggct ctcacctgta cgctccgttc gataaagatt
     7741 gactcatcgg caaacagtac cgcgagcggt agcgagcggg tatcgctctc attagggccg
     7801 agcctcgcgc gcgtcagacc cgctcgctac cgctcgcggt actgtgcgtg ccggcaaatc
     7861 gattctactt gaccttgccc ggcccccaaa gcgcgcggcc cggtgtggcg ccggtgtgct
     7921 cgccgtcgcg caagaccggc acgccgttga cgaggacgtg gcggacgccg accgagtatt
     7981 gatgaggacg gtcgaacgtc gcgcggtcgg cgacctttgc ggggtcgaag acgaccacgt
     8041 cggcgaacat gcccggccgc aggaggccgc ggccagtcag tccgagattc gtcgcgggca
     8101 gactcgtcag ccgccgcacc gcctcagtga gcgtgatgac gcgctcctcg cggacgtact
     8161 tgcccaaaag ccgcgcgaag ttgccgtagg cgcgcgggtg cgtggacgac ttcaggaaga
     8221 cgccctcggg cgccatcgac gaagcgtccg agccgaacga gacccacggc tgacgcagtt
     8281 gcgcgcggat gttttcctcc gacatcagaa agtagacggc ctcgacgcgc gtgccgtcct
     8341 cgacgatcaa atcgagaatc gtgtcctctg cactcgtgcc gcgctctttc gccacggagg
     8401 cgagcgtctt gcccgtcagc tgcttgagcg cctcggtctt gaagcccgag agcagcacgc
     8461 gctccgccga gccggcggcg aggtagaggt tctcccactt gtcggtcggc gtccgcatct
     8521 cttcgagaat ccgccggcgt gtctccgcat ccttgatgcg acggacaagt tcctcgaagc
     8581 ctccgtcctg tgaccacggc ggcagcgcgg ccgtcaggcc cgtcgcgccg gccgggtagg
     8641 tgtacatgtc ggcggtgatc ttgagaccct gccgccgcgc gtcgttgacc atccgcagga
     8701 cgcgcgccat cttcggccag ttgtcccggc ccgccgcctt gaggtgataa atctcggccg
     8761 ggattcccgc ctcgcggctg atgcgaatca actcctcgac ggcctcgatg agtcgcgcgc
     8821 cctcgctacg catgtgcgag atgtacttgc ctcggtagcg cgcggcgacc ttgcacagct
     8881 cgatcaactc ttcggtcgaa gcgtagaacg cgggcgggta gatgagcgac gtgcccacgc
     8941 cgagagcgcc cgcctccatc tcgcggcgca ccagctcctt catctcttcg agctgcgccg
     9001 gtgtcggccg cacgtcgcgc tcgccgagga cgtggatgcg gatggtggtg gcgccgacga
     9061 acgaggcgac gttctgcgag acgccgcgcg cttcgaggtg tcggagatac tcggcgagcg
     9121 tcgtccactc gaccgcgaac ttgatgtcgc cctgatcggc gaccatctgc cgcttcatcg
     9181 cgtcgctcaa aggccccatc gaccagccct cgccgaagat ttgcgtcgtc acgccctgcc
     9241 ggatttcgcc ctgcgagcga gcgtcggcga tgagcgagtc ggttgaccac gagagcatgt
     9301 tgatgaagcc gggggcgacc gccgcgccgc gcgcgtcaac gatggttttg gcgcgctcgc
     9361 gcgagaggtt tccgacaacg gcgacgcggt ctccgcgtat tccgacatcc gcgcggcgcg
     9421 gcgcgcgccc cgtgccgtcg tagaccgtcc cgccgcggac gatgacatcg aactcgccgc
     9481 gcgcgccttg cgcgcggagc ggcgacgaga ggagcaggac gacggcgacg agcgtgatga
     9541 gtcgtttcat agactgattc atatgggccg cttcttcgga gtggattgcg ttcgtggaaa
     9601 ctgtaggggc agggcaagtc ggcggcccgt cctggcgaaa gaataattaa catggatggg
     9661 caggatgggc aggattaaga aagactgatt tcagattgga gatttaagat ttcaaatcat
     9721 ctcatcctgc atctcctgtc catccatgtt aattgcttcc cacgatgcgg gccgcggact
     9781 tgccccgccc ctacagtatt cccacttcgg tgcttgcatc cgcggcgctg tggtcttcgc
     9841 cgaacacgcg cgcgaagatc gcgtccacgt tacgcaggta ggtgtcgggc gagaagacgc
     9901 tttcgattcg ctcgcgcggg agcttagagt tgatgtccgc gtcttcgagc acgagggcgc
     9961 ggaagtcttc gccctcgtcc cacgcccgca tggcgttgcg ctgcgcccac aggtaggcgt
    10021 cctcgcgcga gacgccggcg cgcgtgagcg agagcagcag ttggccgctg aagacgaggc
    10081 cgcgcgtggc gtcgaggttc tcaagcatgc gttcggggtg gacgacgagg gtgtcgagga
    10141 gcgaagtggt cttggccagc aggtagtcga gcgcgatgga ggcgtcgggc agcacgacgc
    10201 gctcggccga cgagtgagag atgtcgcgct cgtgccagag ggcgttcgac tccagccccg
    10261 cgacggcgta gccgcgcatc acgcgggcca tgccgcagat gcgctccgag aggatcgggt
    10321 tgcgcttgtg cggcatcgcc gacgatccct tctgccccgc gcggaaacgc tcctcggcct
    10381 cccgcacctc ggtgcgttgc cagtgacgca cttgcagggc gaacttctcc agcgacgagg
    10441 cgacgatggc cgccgtcgtc agatactcgg cgtacacgtc gcgctgaatg atctgcgtcg
    10501 agaccggcgc ggcccgcagg cccagacgcg cgcacacacc ctcctcgacc gccgggtcga
    10561 ggtgcgcgaa cgcgccgacc gcaccgctga tcttgccgac ggccacggcc tcgcgcgcgc
    10621 gcacgaggcg cgcatggttg cggcgcatct cgtcgtacca gagcgcccac gtgagaccga
    10681 acgaagtcgg ctcggcgtgg acgccgtgcg tgcggcccac ctgcggcgtg cggcggaact
    10741 cgtaggcgcg gcgcttgagg acttccatga gcgcgtcggt gcgtccgagc agtaggtcgc
    10801 aggcgtcgcg cagcagcagg gcgttggccg tgtccaccac gtccgaggag gtcagcccgt
    10861 agtggacgaa gcgcgcggcc tcgccgattg attcggccag cgccgtggtg aaggcgatca
    10921 cgtcgtggtc ggtcgtcttc tcgatctcgt tgatacgctc gaccgtgaac gtagccttct
    10981 tctttatttc cccgacggcc tcccgcggaa tcgtccccat ctcggcgtgg acttcgcaga
    11041 cggccaactc cacgtcgagc cacttctgaa atttattctg ctccgaccag agcgcgccca
    11101 tctcgggcag tgtgtagcgt ggaatcatag ttcaaagttc aaagttcaaa gttcaaagtt
    11161 caaagtcaac agccgccaga cgcgctttga actttgaact ttgaactttg aactcacttg
    11221 agtgcgtctg acgtgacggt gtagccggcg aggagccagc ggccgtcgcg ctcaaggagc
    11281 gtgaaggtct ccatgccggt gccgcgctcg aaggtcgtct ggtaggtgac gacgagcgag
    11341 tggccggtga gggggccggc ggctgtttct ttttcggtgc cggtgtggag cgcgcggctc
    11401 tgcacccgtc cgagacgcgt gcggacgcgc tccaaattgt cgcggctctc ttcggccgtg
    11461 acgacggcgc gccactcgtc ggccgccccg tcgtagagtt cccggtcgcg cgcagcggcg
    11521 atctcttcgg tcacgcggtc gatggccgcc tgctgctcgg gcgtcacttg ccggcgctcg
    11581 tcaacctcgc aggcgaggaa ggcaaaaggc aaaagtaaaa aggcaaaagt gaagagaact
    11641 tgacgcgtgc tcggtgtcct cacttttgcc ttttgccttt ttacttttgc cttcatatct
    11701 cgatccaggg ggtcggctcc ttcgggtgcg agagcgtgat cttagtggtg gtctggaaga
    11761 gacgcccgcg ctcctggagg gcgatttcgg cggagagctt ggcgacgtag gggttgttgg
    11821 cgcgctgcga gaggtgggct aagacgacgt gttcggccgc gccgtcgaag tcgtcgcgca
    11881 gccacacggc cacgtcctcg ttcgagagat ggccggtgcg cgacagtatc cgctgcttga
    11941 gttcccacgg gtagacctcg cacgccttga gcatgtcgcg gctgtggttc gactcgatga
    12001 cgatggcggc gcaagccttc agacgctcgg cgacgagcgt ggtgacgtgg ccgaagtcca
    12061 tcagcgtggc gacgcgcacg ccgcccgagg aggcgacaaa gccgaagttg tcggcggcgt
    12121 cgtgcgggat ggtgaagggg tggaagtcga tctcgccgat gcggaaatct ttgctcgaag
    12181 atatttcctc ggtgcggtcg cgcacggcgg cggcgcgctt gcgcggctcg tccttggcgg
    12241 acgagccggc gtgttcgcgg atgtaggcgt cgcgcgtctc gccggagatg tagacggggc
    12301 aactcaggga tttgagcagc acgcgcaggc cgccggcgtg gtcgccgtgc tcatgcgtga
    12361 cgatgacgcc gtcgagggcc tgaacgtcct cgccgacgag cgcgaggcga cgcgccagct
    12421 ctttcgcgct caggcccgcg tccacgagca cgcgggtgcg gcccgcgcag atgagcacgg
    12481 cgtttcccgt ggagccgcta cctagaacag tgaagcgcat aaaagccgtg ataagtgaca
    12541 agtgataagt gataagttta agacatgctc ttcaacttgt cactaaacac tcaccacccg
    12601 tcacttatca ctcatcactt atcactcatc acttatcacc gttttacctc gtgagcacaa
    12661 gcttcgagac cttgtggccg ccggcgatgt agag
//
LOCUS       DASVES010000161         8245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1694860, whole genome shotgun sequence.
ACCESSION   DASVES010000161 DASVES010000000
VERSION     DASVES010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694860"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VF240_06520"
     CDS             complement(<1..728)
                     /locus_tag="VF240_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX7174917.1"
                     /translation="MEEEYGLQHGGPLPPAAQRLVAACNDLGLGLLRELLKAGEGRNL
                     LVSPFALSLALAVLYNGAGGDTRRVLRGVLGAHDIGDEEFNAAFMSLRRLLTGGADVT
                     LSLASALWVREGLRFEAPFVQAVGHYYGAEAAALDFDSPTALDAVNQWVREQTAGKIG
                     GVIKSDDISPATDLVLASIAYFKGQWANPFDRASTREAPFHLADGRSRSVPMMHREGS
                     FKFYQTPLFQAVRLPYGDGRLSMD"
     gene            complement(759..1646)
                     /locus_tag="VF240_06525"
     CDS             complement(759..1646)
                     /locus_tag="VF240_06525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX7174918.1"
                     /translation="MDEDNQTTQMFDELQDRLNHLESEVGELRRKSGGGGQRSMPNRR
                     DEVVSPDPFRRSEVFPPSTQGEPESIVGGKMTTDFPDCCAVGDDTEFYCTGTLIAPRL
                     VVTAQHCPDLRRVFLKGNDVSRLDTGEVIRVVDNKEHPSNDLRVLVLERDSAVKPRHV
                     AQGAEVGNPKKARLVGFGTVNLGGTIGYGVKRMVDVPITSLGCESANDQTKFGCSGGK
                     EMVAGHKGLLKDSCRGDSGGPLYIKSSTGAYYLLGATSRGLGNTNNVCGDGGIYVRVD
                     KFLDWIRQETGIDVEGPLD"
     gene            complement(1708..3153)
                     /locus_tag="VF240_06530"
     CDS             complement(1708..3153)
                     /locus_tag="VF240_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174919.1"
                     /translation="MANLQQLLATELRRRTELLPREIDAWNDQSDANKYGMGLHQSQV
                     KVVTKLFSTMLAEQDALKEKLDPKEVATMPQDTFTSKRMELEQMLTGSHSIMSIFRYI
                     FSQRDATQPYGGILDAADLVAAACYLPCVKLHNRWKVLPEENYREPPLTYLNAKLSPA
                     AITRRHYFGSIGLELQGEEELQLPLSVISLAFHDTTGFWELSSIYHEVGHLLTQDLKL
                     RDDLRKAMETKLAGRAKLDDWKRWLNEMIADAFGVLLGGAGFARGLFAMLYKTKAEVL
                     AASGVGEHPNSYVRVFLLGALLRSTGVAELQTLADTIEGDWRNFYGPADATLKPYVDD
                     CGAVADVLMSEPLPVLEKDRCLLDFARDKTSGVNSAPDLEKDNRYAAALARFIRLGKM
                     SNDYNEAMLQPADLIRLVPAAAQLAVQGVDAEHEQKFEDIHKAALELTLRLQKEAKIE
                     FLAGEDTGARDAYLDGLIKQVNFKAIRIDQP"
     gene            complement(3169..5061)
                     /locus_tag="VF240_06535"
     CDS             complement(3169..5061)
                     /locus_tag="VF240_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX7174920.1"
                     /translation="MTTDFEVDILPDNSAGTNLLIRLTASDPELSVNRKEFLREVAID
                     IDANTIRDLRGGNPTSAVVNAVAQKLSDWLISSDIRGYVNGPILGTKPVRFIFRFHPR
                     LLEKFADVPVELLKFEDEWVVLRHAVSAIVHELPGTQLSKPFYHSLPLKILVVRSSPS
                     GLTVRVPPAGPICNHIVKLANELGQNIVHIDLISGEARSLEPQLSWDEFEAKHLPNLV
                     IADGDSEEERQRKESLKWREFRDYLLNRGVTELQPATWEKFTDHLKNSSTNYHILVYL
                     GHGNYLNLDGSGKKSGVLQFEKPGGVNVDNISSNDLQRQLQDRQVAIPVVMLAGCLTA
                     AENEPPLDAEELKDIAEWTLGSQGVAQRLVGTSSGVQCAVGMRYQIKIGAAFDFLATF
                     FESLLRETPGNVEAAVRAGRVKISRPEYPPSWSAPVIFRTRGAEPMFNFDIPRTYKLD
                     RDNIRDQDVREAAWTALSEDPTSDFAYETLAEIEKLIKAKATKKQTSLLMPEMKKAAP
                     DEVVEVAVNLVGALQADKLDCKLSVSSDHGNARIESLHSTQPFKDSKFSLGNMSESGG
                     SDITFTITRGAGAFPLPEGPILVVRLRLAPVAPAKHTVRLDVVEVVPPNVKVRPIDNV
                     VLVSGK"
     gene            complement(5061..6674)
                     /locus_tag="VF240_06540"
     CDS             complement(5061..6674)
                     /locus_tag="VF240_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174921.1"
                     /translation="MGKRQSRYEEVVVWVAPPGGRGAAQAPAPPVKAPDSDADGFWER
                     LGLWFRTRVSESKLSEHRHFDAWVYTDIELPAHDTAESCDQENAPAFTTFKFWRRGRI
                     TLDQRKYNDYLRTFMSGESAAYKKLWSFPFRNESHAAAIKAARDSIFSVPDQSLSDLL
                     NGKVALPERPVRIWWNCTPPELAELPWELLACELREKRQGLFSFVRGLPAAPLHKIPV
                     KEKLRLAFIHDPERTPDALKAAVEGLDKSHIEVVGFTQPPREALKRVSEEGFELVHLV
                     ADGAVSFGGEGLLYFPKWDAARTAERLGPATRRLFRIALNYSTKLRYIVSDERLLKWN
                     DSLSRRLDIEPCSAGEMCSILRGSRVTLLSLSTTKTDDDDIDRLDGPLLPSVYSAYAA
                     IGSSTITLPNIIAPLGPCGGDLLGKFWRYFYEHLASPDGYSIEDGMAAGTTGAPTVLM
                     SLFLRQRLGREFTARADTRTPATQDPTRVNARLEVARSLLEQLRAIDQSYKDIGSDVS
                     DTPLVKDESARQTMIEQELGSLSELEEGE"
     gene            complement(6765..7112)
                     /locus_tag="VF240_06545"
     CDS             complement(6765..7112)
                     /locus_tag="VF240_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174922.1"
                     /translation="MKAGSDEAALGAGDDRSHRPRRKLPTLSAGGAGATPSHRIWLYK
                     LWRLIFAWLFVIVSAAIAIYTGFSQLYIGQAFGTWQDYVKIFLWGFGAQATLTAVLAG
                     LNLLWHSRVLRFS"
     gene            complement(7213..>8245)
                     /locus_tag="VF240_06550"
     CDS             complement(7213..>8245)
                     /locus_tag="VF240_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7174923.1"
                     /translation="KKLKLRAQFANGDGLRSGSEVRLAGVRIGKVDKVELLPPAGDRG
                     AKVEATMSIDSEIHGKPAADLIRTDSFATLGSPSLLGSDKLVNVAPGSPVGEPVPDTR
                     EGYLLPLGKDSAGFGALTESGNELMQQLNKLSVEFTAMAEGLNRGEGTIGRFLNDEAF
                     YNNLNATIRDTQTLIREIESGKGSAGKFVNDPALYDNINGISVQLQSIATDLRAGRGT
                     AGKLLTDDALYNDVRSTVGRFNSSVDEINLIVGDLRAGRGTAGKLLTDEAIYNDARAA
                     IARVNTAAERLDNVVSGVQRGEGTVGKLFTDDQLYSNFNQLSSESVKLIYDFRQNPRK
                     YLTIKFELF"
BASE COUNT         1290 a         2564 c         2794 g         1597 t
ORIGIN      
        1 aagtccatgc tcaggcggcc gtcgccgtaa ggcagacgga cggcttgaaa caaaggcgtt
       61 tgataaaact taaaactgcc ctcccgatgc atcatcggca cacttcgact tcgcccatcc
      121 gcgaggtgaa aaggcgcttc acgtgtcgaa gctctgtcaa acgggttggc ccactggcct
      181 ttgaaataag cgattgatgc cagaacgagg tcggtcgcgg gcgagatgtc gtcgctcttg
      241 atgacgccgc caattttgcc cgcggtctgc tctcgcaccc attggttgac ggcgtcgagc
      301 gcggtcgggc tgtcgaagtc cagcgcggcc gcctccgccc cgtagtaatg gccgacggct
      361 tggacgaaag gggcttcgaa acgaagcccc tcccgaaccc acaatgcact ggctaagctc
      421 aacgtaacgt ctgcgccgcc cgtcaggagg cggcgcagcg acatgaaggc ggcgttaaat
      481 tcctcgtcgc cgatgtcgtg cgcccccagc acgccgcgca acacacgccg cgtgtcgccg
      541 ccggctccgt tgtacagcac ggctaaagcg agggagaggg cgaagggaga caccagaagg
      601 tttcggccct cccccgcctt cagcagttcg cgcagaagcc cgaggccgag gtcattacag
      661 gcggcgacga gcctctgggc ggcgggaggt aaaggcccgc catgctgcaa tccgtactct
      721 tcttccatcg cttcccggtg cctctgcggc gaggagactc aatcgagcgg gccttcgacg
      781 tcgatcccgg tctcctggcg aatccaatcg aggaacttgt caacgcgcac gtagatgccg
      841 ccgtcgccgc acacgttgtt ggtgttgccg agtccgcgcg aagtggcgcc gagcaggtag
      901 taagcgccgg tcgagctttt gatgtagagc ggcccgccgc tgtcgccgcg gcaactgtcc
      961 ttgagcagcc ctttatgacc tgcgaccatc tccttcccgc ccgagcagcc gaacttcgtc
     1021 tgatcgttcg ccgactcgca tccgagcgag gtgatgggca cgtccaccat gcgcttgacg
     1081 ccgtagccga tggtgcctcc gagattgacg gtgccgaacc ccacaaggcg cgctttcttc
     1141 gggttgccta cctcggcccc ctgcgccacg tgccggggct tgaccgccga atcacgctcc
     1201 agtaccagga cgcgcaggtc gttactcggg tgctccttat tgtccacgac gcggatgacc
     1261 tcgccggtgt cgagccggct cacgtcattc cccttaagga agacgcgcct gaggtcaggg
     1321 cagtgttggg ccgtgacgac gaggcgcggc gcgatgagcg tgccggtgca gtagaactcg
     1381 gtgtcgtcgc cgacggcgca gcagtcgggg aagtccgtgg tcatcttgcc gccgacgatt
     1441 gactcgggct cgccctgcgt actcggcggg aaaacctcgg agcgtcggaa cgggtcgggg
     1501 gagaccacct cgtccctccg gttgggcatg ctccgctgcc cgcccccgcc ggacttgcgg
     1561 cgcagctccc ccacctcgga ttcgaggtgg ttgaggcgat cctgcaactc gtcgaacatc
     1621 tgtgtcgtct ggttgtcttc gtccatcggt tctctccttc gtgcgtgggc cgggccccgc
     1681 ttgattgttg aaagctaccc tcatctatca gggctggtca attcttatcg ccttgaagtt
     1741 gacctgtttg ataaggccgt cgaggtatgc gtcgcgcgcg ccggtgtcct caccggcgag
     1801 gaactcgatc ttcgcctctt tctggaggcg gagcgtcagt tcgagcgccg ccttgtggat
     1861 gtcctcgaat ttctgctcgt gctcggcgtc cacgccctgt acggcgagct gcgcggccgc
     1921 ggggaccagc cggatgaggt cggcgggctg gagcatcgcc tcgttgtagt cgttgctcat
     1981 cttcccgagc cggatgaatc tggcgagcgc cgccgcgtac cgattatcct tttcgaggtc
     2041 gggggcgctg ttcaccccgc tcgtcttatc gcgcgcgaag tcgagcagac agcggtcttt
     2101 ttcgaggacg gggagcggct cgctcatcaa cacgtcggcg accgccccgc agtcgtccac
     2161 gtagggcttg agggtcgcgt cggcggggcc gtagaaattc cgccagtcgc cctcgatcgt
     2221 gtcggccagg gtttgcaact cggcgacccc cgtgctccgc agcagcgcgc cgagcaggaa
     2281 gacgcgcacg tagctgttgg ggtgctcgcc gacgccgctc gcggcgagca cctccgcctt
     2341 cgtcttgtag agcatggcga agagtccgcg ggcgaagccc gcgccgccga gcagcacgcc
     2401 gaaagcgtcg gcgatcatct cgttgagcca cctcttccag tcatccagct ttgcgcgccc
     2461 ggcgagcttt gtctccatcg ccttcctcag gtcgtcgcgc agcttcaggt cttgcgtgag
     2521 gaggtgaccg acctcgtggt agatgctcga cagctcccag aagccggtcg tgtcgtggaa
     2581 ggcgagcgag atgaccgaga gggggagctg caactcctcc tcgccctgaa gctccaggcc
     2641 gatgctgccg aagtagtggc ggcgcgtgat ggcggccggc gagagcttgg cgttcaggta
     2701 ggtgagcggg ggctcacggt agttctcctc ggggagcacc ttccacctgt tgtggagctt
     2761 gacgcagggc aggtagcagg cggcggcgac gaggtcggcg gcgtcgagga tgccgccgta
     2821 gggttgcgtg gcgtcgcgct gcgagaagat gtagcggaag atcgacatga tgctgtgcga
     2881 gccggtcagc atctgctcca gctccatccg cttgctcgtg aaggtgtcct gaggcatcgt
     2941 ggccacctcc ttcgggtcca gcttctcctt cagggcgtcc tgctccgcga gcatcgtgga
     3001 gaaaagcttg gtcaccacct tgacctgact ctggtgcagg cccattccgt acttgttcgc
     3061 gtcgctctgg tcgttccagg cgtcgatctc gcgcggcaga agttcggtgc ggcggcgcag
     3121 ttccgtcgcc agcaactgct gtaggttcgc cataaaattc ctccggggtc acttgccgga
     3181 cacgagcacg acgttgtcga tggggcggac tttcacgttc ggtggcacga cctccaccac
     3241 gtcgaggcgg acggtatgtt tcgccggggc gaccggggcg agcctcagcc tgacgacgag
     3301 gatggggcct tcgggcagcg ggaacgcgcc tgcgccgcgc gtgatggtga aggtgatgtc
     3361 gctgccgccg gactcgctca tgttgcccag tgagaacttc gagtccttga agggctgtgt
     3421 gctgtgcagc gactcgatgc gcgcgttccc gtggtcgcta ctcacgctga gcttgcagtc
     3481 gagcttatcg gcctgaagcg cgccgacgag gttgacggcg acttcaacca cctcgtccgg
     3541 cgcggccttc ttcatctcgg gcatcaggag cgaggtctgc ttcttcgtcg ccttcgcctt
     3601 gatcagcttc tcgatttccg ccagcgtctc gtaggcgaag tccgaagtgg ggtcttcgct
     3661 gagggccgtc cacgcggcct cgcgcacatc ctgatcgcgt atgttatcgc ggtcgagctt
     3721 gtaggtcctc gggatgtcga aattgaacat cggctcggcc ccgcgcgtgc ggaagatgac
     3781 gggcgccgac cagctcggcg ggtactcggg gcgcgaaatc ttcacgcgcc ccgcgcgcac
     3841 ggcggcctcg acgttgcccg gcgtctccct cagcagcgac tcgaagaagg tggccaggaa
     3901 gtcgaacgcg gcgccgatct tgatctggta gcgcatcccg acggcgcact ggacgccgga
     3961 cgaggtgccc acgagccgct gcgccacgcc ctgactgccc agcgtccact cagcgatgtc
     4021 cttaagctcc tcggcgtcga gcggcggctc gttctcggcc gccgtcaggc acccggcgag
     4081 catgaccacg gggatggcca cctgcctgtc ctgcaactgc cgctggaggt cgtttgacga
     4141 gatgttatcc acgttgacgc cgcccggctt ctcgaattgc aggacgccgc tcttcttccc
     4201 cgagccgtcg aggttgaggt aattgccgtg acccaggtag acgaggatgt ggtagttcgt
     4261 gctgctattc ttgagatggt ccgtgaactt ctcccacgtc gcgggttgca gttccgtgac
     4321 gccccggttc aggaggtagt ctcggaactc tctccacttg agcgactcct tccgctgcct
     4381 ctcttcctcg gagtcgccgt ccgcgatcac caggttgggc aggtgcttgg cctcgaactc
     4441 gtcccaagag agctgcggtt cgagcgagcg ggcctcgccg ctgatgaggt ctatgtgcac
     4501 gatgttctgc cccagttcgt tcgcgagctt gacgatgtgg ttgcagatcg ggccggccgg
     4561 cgggaccctg acggtcaggc ccgaggggct cgaccgcacg actagtatct tgagcgggag
     4621 actatggtag aaaggcttgg acagctgcgt gcccggcaac tcgtggacga tggccgagac
     4681 cgcgtggcgc agcaccaccc attcgtcctc gaatttgagc agctcgacgg gcacgtccgc
     4741 aaacttttcc agcaggcgcg ggtggaacct gaagatgaag cgcaccggct tggtgccgag
     4801 gatcggcccg ttcacgtagc cccgtatgtc gctggagatg agccagtcgc tcagcttctg
     4861 ggcgacggcg tttacgacgg cgctcgtcgg gttgccgccg cgcaggtcgc ggatggtgtt
     4921 ggcgtcaatg tctatggcga cctcacggag gaactccttg cggttgacgc tcagttcggg
     4981 gtcgctcgcc gtgaggcgga tcagcaggtt cgtgccggcg ctgttgtccg gcaatatatc
     5041 gacctcgaaa tcggtcgtca tcactcgccc tcctccaact cgctgagcga gccgagctct
     5101 tgctcaatca tcgtctgcct tgcgctctcg tccttgacga gcggcgtgtc ggagacgtca
     5161 ctgccgatgt ccttgtagct ctggtctatg gcgcggagct gttcgagcag gctgcgcgcg
     5221 acttcgagcc gcgcgttgac tcgggtcggg tcttgggtgg cgggggtgcg cgtgtcggcg
     5281 cgcgcggtga actcgcgccc cagccgttgg cgcaggaaga gggacatcag cacggtcggc
     5341 gcgcctgtcg tccctgcggc catgccgtct tcgatgctgt agccgtcagg cgacgccaga
     5401 tgctcgtaga agtatcgcca gaacttcccg agcaggtcgc cgccgcaggg cccgagcggg
     5461 gcgatgatgt tgggcagtgt aatcgtcgag ctgccgatgg cggcgtaggc gctgtagacg
     5521 gagggcagca gcgggccgtc gaggcggtcg atgtcgtcgt cgtcagtctt ggtcgtactc
     5581 aggctcagga gtgtgacgcg gctgccgcgc aggatcgagc acatctcgcc cgccgagcag
     5641 ggttcgatgt cgagccgccg cgagaggctg tcgttccatt tgagcagccg ctcgtcgctg
     5701 acaatgtagc gcagcttcgt cgagtagttg agggcgatgc ggaagaggcg gcgcgtggcg
     5761 gggcccagcc gctcggccgt gcgggcggcg tcccacttgg ggaagtagag aagcccctcg
     5821 ccgccgaacg agacagcccc gtcggccacg aggtggacca gctcgaagcc ctcctccgag
     5881 acgcgcttaa gggcttcacg cggcggctgc gtgaagccga cgacctcgat gtgtgacttg
     5941 tcgagtccct ccaccgcggc cttcagcgcg tcgggcgtcc tctccgggtc gtggatgaag
     6001 gccagccgga gcttctcctt gacgggaatc ttatgcagcg gggcggccgg gaggccgcgg
     6061 acgaaggaga aaagtccctg cctcttttcg cgcagctcgc atgccagcag ctcccacggc
     6121 agctcggcca gctccggcgg ggtgcagttc caccagatgc gcacggggcg ttcgggcagc
     6181 gcgactttcc cgttgagcag gtcgctgagg ctctggtcgg ggacgctgaa gatgctgtcg
     6241 cgcgcggcct tgatggccgc ggcgtgcgac tcgttccgga aggggaagct ccagagtttt
     6301 ttgtaggcgg cactctcgcc gctcatgaaa gtcctcagat agtcattgta cttccgctgg
     6361 tcgagtgtga ttcgcccgcg ccgccagaac ttgaacgtcg tgaacgcggg cgcgttctcc
     6421 tggtcgcagg actcggcggt gtcgtgggca ggcagttcga tgtccgtgta gacccacgcg
     6481 tcgaagtggc ggtgctcgga cagtttgctt tccgagaccc gtgtgcggaa ccacagccct
     6541 aagcgctccc agaagccatc cgcgtcggag tccggagctt tgacgggggg cgcgggcgcc
     6601 tgcgccgccc cgcgtcctcc ggggggcgcg acccaaacga ccacctcttc ataccgggat
     6661 tgacgtttgc ccatgctgtt cctcccggcg tgcacacgca tgccgcctga cggattaaat
     6721 cgctctcttg cgggcgccgc gtgcccgcta cttcgcggcc cggctcaact gaagcgcagg
     6781 acgcgcgagt gccaaaggag gttgagcccc gcgaggacgg ctgtcagtgt ggcttgggcg
     6841 ccgaaccccc acagaaagat tttgacgtaa tcctgccacg tgccaaaagc ctgcccgatg
     6901 tatagctgac tgaaccccgt gtagatggct atcgccgccg aaacgatcac gaacagccag
     6961 gcaaagatga gacgccagag cttgtagagc cagattctgt ggctcggggt cgcccctgcg
     7021 ccacccgcgg agagcgtcgg cagcttgcgc ctcggcctgt gagagcggtc gtccccagcg
     7081 cccaacgcag cttcgtcgga gccggccttc acgaaatgcg ggctgagtag ctgagtctcc
     7141 ctttccgcac ctgtcaggcc ggaaagtata tcttcaatgc gttaatcggc gcgcgcccac
     7201 aaccggctcc gcctaaaata actcaaactt aatggtcagg tacttccggg ggttctggcg
     7261 gaagtcgtag atgagtttga cgctttcgga ggagagttgg ttgaagttgg agtagagctg
     7321 gtcgtcggtg aagagcttgc cgacggtgcc ttcgccacgt tggacgccgg agacgacgtt
     7381 gtcgaggcgt tcggcggcgg tgttgacgcg ggcgatagcg gcgcgggcgt cgttgtagat
     7441 ggcttcgtcg gtgaggagct tgccggcggt tccgcggccg gcgcggaggt cgccgacgat
     7501 gaggttgatc tcgtcaacgg agctgttgaa gcggccgacg gtggagcgca cgtcgttgta
     7561 gagcgcgtcg tcggtgagga gcttgccggc ggtgccgcgc cccgcccgga ggtcggtggc
     7621 gattgactgg agttggacgc tgatgccgtt gatgttgtcg tagagcgcgg ggtcgttgac
     7681 gaacttgccg gccgagcctt tgcccgactc gatctcacgg atgagcgttt gggtgtcgcg
     7741 gatcgtcgcg ttgaggttgt tgtagaacgc ctcgtcgttg aggaagcggc cgatggtgcc
     7801 ctcgccgcgg ttaagcccct cggccatggc ggtgaactcg acggagagct tgttgagctg
     7861 ctgcatcagc tcgttgccgg actcggtgag cgcgccgaag ccggcggagt ccttgccgag
     7921 cggaagcagg tagccctcgc gtgtgtcagg cacgggttcg ccgacggggg agccgggcgc
     7981 gacgttgacc agcttgtcgg agccgagcag tgagggactg ccgagggtgg cgaaggagtc
     8041 ggtgcggatg agatcggcgg cgggcttgcc gtgaatctca ctgtcgatgg acatggtggc
     8101 ctcaaccttc gcgccgcggt cgcccgcggg cggcagcagt tccaccttgt caaccttgcc
     8161 gatgcggacg ccggcgagac gcacctcgga gccggagcgc aggccgtcgc cgttggcgaa
     8221 ctgggcgcgc agcttcagct tcttc
//
LOCUS       DASVES010000162        10412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1001968, whole genome shotgun sequence.
ACCESSION   DASVES010000162 DASVES010000000
VERSION     DASVES010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001968"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VF240_06555"
     CDS             <1..284
                     /locus_tag="VF240_06555"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX7174924.1"
                     /translation="VITRKRLNEFAEKYPETKTALAHWYSLTKHNDFSNFAEVKAMFP
                     FADRVGKLTVFNVGGNKVRLIVAIHYNRKRVYIRGVLTHAEYDEGKWKE"
     gene            288..518
                     /locus_tag="VF240_06560"
     CDS             288..518
                     /locus_tag="VF240_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174925.1"
                     /translation="MQNFDVNKTADALSPLAGTVFVPHTEEEYRKLVALLDGLIDEVG
                     EDESHPLASLTEIVGVLIEKYEDEHVPELTVE"
     gene            716..1078
                     /locus_tag="VF240_06565"
     CDS             716..1078
                     /locus_tag="VF240_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174926.1"
                     /translation="MRSVAHNEEPTKSISINPKSAALISFVLVLPFAILESLNNTINR
                     QNVPALIVLFGLLWLLPMAFILILAPIVRSARAGNGVMANPFNLLFRVAMLALVAMMW
                     GVLLIDQLPCFMGAPNCD"
     gene            1257..1895
                     /locus_tag="VF240_06570"
     CDS             1257..1895
                     /locus_tag="VF240_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174927.1"
                     /translation="MEIIEEPIISAAELARVPIAFEVERVFDVWPRDGGLGGLTLSER
                     RLDVPYVKDYDTLEGEGPAQWAERFDVSRWGLIGARVNGARVGGAVVAFNTVGVQMLE
                     GRRDLAVLWDIRVAPEARGRGVGSALFRAVEAWAAARGCRQLKIETQNINVPACRFYA
                     RQGCVLGAINRFAYPELPDEVQLLWYKTLSHRPPTGLATACTRIQALTCHQI"
     gene            1965..2294
                     /locus_tag="VF240_06575"
     CDS             1965..2294
                     /locus_tag="VF240_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX7174928.1"
                     /translation="MAGAKLVVLYPFPQDVDVFERAYTEEHVPMVNENTMKGMAKFVT
                     TKVLGTADGSAPPFYRMAELHFPSLATLQECASSGSAQAAVAHAISISSGGTPLFLVA
                     EEETTTF"
     gene            2353..2808
                     /locus_tag="VF240_06580"
     CDS             2353..2808
                     /locus_tag="VF240_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174929.1"
                     /translation="MKAKGYRGILWGGLAAGVLDLAAALVTSSLRGVTPTRVLQAIAS
                     GVLGADSYRGGAQSATLGVALHFVIAFGAAAVYYAASRRLKCLVRRPVVSGLLYGVAV
                     YLFMNLVVLPLSAITFKVSYAPAVLATGLVVHMLCVGLPIALAVRRFSK"
     gene            2827..3390
                     /locus_tag="VF240_06585"
     CDS             2827..3390
                     /locus_tag="VF240_06585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174930.1"
                     /translation="MSRSSILADMDYLIRRATLADRAAIERLIAESARGLSRGDYSER
                     QIEAAVAAVFGVDTSLIIDGTYFVAESGRELIGCGGWSRRKTLFGGDQIPGRDAGELD
                     PESEPAKIRAFFVHPAHARRGVGRAILAACETEARSFGFRSLELLATLPGVRLYEACG
                     YEAGEHVWHEAQGGVPVEFVPMRKDLP"
     gene            3519..4559
                     /locus_tag="VF240_06590"
     CDS             3519..4559
                     /locus_tag="VF240_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174931.1"
                     /translation="MKRTLHVAMLALAVASAALASDALAQNKQTRSQNNNTPAGAQPA
                     LPTLPASDAVVLVELRRLISEELPRALASDAPRLAAVNADIDAFKTRTGIDPRAFDTL
                     AAGSRFMSLAGGKTKLTHTVAVARGTFDMAAIVAALRASSKGNHREQKHAGKSVHVFT
                     LNEEVKLFGLLGVRVRDLAVAELDRTTLAVGHPAAVLAAIDAARGRGALRASDLTVLT
                     QPRAASTLVAFGGKVSATAFKDIDVGTPEISRSVASIREFYGALSTTAGGYGMQTTLR
                     TTGAADAKSLGDTIGALKQLAPLFIGRLSGDRQRLAQSAVESLQVSAQGNEVRLSLEI
                     AQGDITTLVRTF"
     gene            complement(4689..6728)
                     /locus_tag="VF240_06595"
     CDS             complement(4689..6728)
                     /locus_tag="VF240_06595"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX7174932.1"
                     /translation="MLKLFSRMERTRSYVIIAFALLMGLSLIFFYAPSQNSAVSTSGM
                     TREVLARVRGDSITVGELEDLKSGYRKMFGAQFNPAQFGGDRRMLDDLIRGRIVAQEA
                     ERLGLAPSDGEVRDAVIKRYTNPQTNQFVGAERYKQLALSQYGSVKRAEQQIRDALAE
                     EKLRAFVTAGASVSEEEVQREWERQNTKFELLHVPVLPSDLARTLTPTDAELAAYYDA
                     HKDEFKIDVPQKNIRYVFVSQSKAGERAQIPDEELRREFDALKPENKVAGVRVQQIVL
                     KVAAKELDTEVLNKATALVASIRGDDLKASEEKFADLARGRSEDPATAKAGGWLPRPV
                     KKAADKTGDIYQNALSLEAGQVSDPFFDKKASAYYIFRRGEQIDKTFEEAKQELLVSL
                     RNRRGYAAAAQVAQRAAERLKETKNHEQVAQELAAEANMSAAEMVRETGFVKPGDEVK
                     DIGSSPQFEDAIAPLNNPQDVGERVSIRDGFAIPMLLEKRDPRVPELAEVREKVAEKV
                     KAEKATAQVEAAARELAAANTPDELKAIAERLGLKPQESKEYTLGTPLGAAGTSGAAD
                     EAIYALRPGNVTKTPIKIEQTYVVAAAKGRTDADLAKFGAERDKLIASALDERRMQVF
                     DDYMTSVRARLDREGDVKVYDDVLAKVAALEAPNIAAPRSPLGGGASAPFPVIPE"
     gene            7044..8159
                     /locus_tag="VF240_06600"
     CDS             7044..8159
                     /locus_tag="VF240_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7174933.1"
                     /translation="MTWSGYPIWAWMAFAVFILLMLALDLGVLNRKAHAVTYREAATW
                     SAVWVSLALVFAGLVFWQNGRLTGAEFLTGYIIELSLSVDNLFVFLLIFGYFKVPAKY
                     QHRVLFWGVMGALIMRMTMIALGAALIERFHWIIYVFGVFLVYTGIKMMRQEETAANP
                     EDNPIVRFVSRLVPMTRHYDGEKFFTEVNGKRMGTLMLLVLITVEVTDLVFAVDSIPA
                     IFGITQNTFIVYTSNVFAILGLRSLYFLLAGVIEKFHYLKIGLSVVLAFVGLKMLTEG
                     FIIGTTIGGYTVQKEAVIVVSLAVVALVLMSSVAASYIWPKSAETHVEVELPPGFGSP
                     YDDDQGQLGGEDRPLPAAEVKSQKEIGKSAIAGPETE"
     gene            8431..9669
                     /locus_tag="VF240_06605"
     CDS             8431..9669
                     /locus_tag="VF240_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX7174934.1"
                     /translation="MATRISRFDLEWWIVSKRHIYLVILLVVLSLAAGGGSVYLWFYG
                     NPFRGAVETDAKAEGARFDSFEGDVRVTRASTRETFPARSETSLYPGDIVQTQEDGRA
                     RITLADGSTLLIKPNSVITIAENTRSDSDGLANVRVAVDRGHVNVRTDQQAENASNIV
                     KTPLTENELAAQTDASFGVRDDKQEEIRVATGSVETSTRNGERATFSNGEFALVSQSA
                     DIVQKERLIDAPAPTAPRNLERIAARRGAATSVALRWQKPANGNPTHYRVEVATSPFF
                     VDPGKVIERDQLPTLTFTVGDLQQGNYFWRVRAVAQSGQASEWSEPQKFVVVPEGGGG
                     EAATVSNVTVEYVAGNVYIIRGQTQPGTNVYCAGKQTLAGSGGNFELQIAAPHGARDV
                     TLEAAGTQGARGSYRVALQQ"
     gene            9796..>10412
                     /locus_tag="VF240_06610"
     CDS             9796..>10412
                     /locus_tag="VF240_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174935.1"
                     /translation="MKHVILIGDEQGPPAGALVESLRAAGFLAAAQALPAAGAGSSGN
                     GSDESSCPTAVLVEVASGADLSHVHDAAQRASAAWPGSPLVAFRSDKAPQNGHAPHAH
                     LDTSTLERLGFHAVADEPAQLPALLRALESRGLTGELRAPEPDERTAARAAETPPASL
                     LLPERLGVEQLRVAFETVASLHFSNDQRSAASSALVGLAALVAAER"
BASE COUNT         1726 a         3437 c         3291 g         1958 t
ORIGIN      
        1 atgtaattac tcgcaagcgg ctcaatgagt ttgccgagaa ataccccgaa acgaagacag
       61 ctctggcaca ttggtatagc ctgacgaagc ataacgactt ctcaaatttt gctgaagtga
      121 aggcaatgtt tccgtttgcc gaccgagttg gcaagctgac cgtcttcaat gtcggcggga
      181 acaaggttcg cctaatcgtg gcaatccact ataatcggaa gagagtctat attcgcggcg
      241 tgcttactca tgccgaatat gatgagggga agtggaagga ataggtcatg cagaattttg
      301 atgtgaacaa gacagcagat gcgttgtctc cgctggcggg gacggtcttc gtgccgcaca
      361 ccgaggagga ataccggaag cttgtcgcat tgctcgacgg cctgattgat gaagtcgggg
      421 aagatgagtc ccacccgctc gcatcactga cggagatcgt tggtgtcctg attgagaagt
      481 atgaagatga gcatgtgcca gagttgacag ttgagtagtt ccctcaatcc gcagcccaac
      541 aagtcattcg agccggccgc gagttagcgc gcctctcatc gttagcttgg atgatttcga
      601 ggttgtgtgc gcggcagctc aattccggcg ttggggggcc atcgttagaa ttcgatgcgg
      661 cgatagacaa atcgcttcaa gtagaacatc attttcaaaa gaaggagatt tgccaatgcg
      721 aagtgttgcg cacaatgaag agccgacaaa aagcatctct ataaacccaa aatcggcggc
      781 gctcatcagc tttgtcctcg tgctgccgtt cgcgatcctc gaatctctga ataacacaat
      841 aaacaggcag aatgtccccg ccctgatcgt cttgtttggc ctcttgtggc tcttaccgat
      901 ggccttcatc ctcatccttg cgccgatagt gcgaagcgcg cgcgcgggga acggcgtgat
      961 ggcgaatccc tttaatcttt tgttcagagt cgctatgttg gccctcgtcg caatgatgtg
     1021 gggggtcttg cttattgacc agctcccctg ttttatgggc gctccgaact gcgattagat
     1081 agttttaccc ggcgagccaa aagataggcg agtgtgtgct atccacgcgc cgcccaacaa
     1141 gtcgttcgag ccggccgcga gtttgcgttg ccctcattgt tatcttgggt ggtttcggcg
     1201 ttgtgcgcgc ggtggctcaa ttccagcgtt agccctccgc aggcgagtag ttgaatatgg
     1261 agatcatcga ggagccgatc atttcggccg cggagctggc gcgcgtgccc atcgcgttcg
     1321 aggtcgagcg cgtttttgac gtgtggcccc gcgacggcgg tctcggcggg ttaacgctgt
     1381 cggagcgccg gctcgatgtc ccctacgtga aggactacga cacgttggag ggtgaaggcc
     1441 cggcacaatg ggctgagcgc ttcgacgtgt cgaggtgggg attgatcggg gctcgcgtga
     1501 acggcgcgcg tgtcggcggg gcggtcgtcg ccttcaacac cgtgggcgtc caaatgctgg
     1561 agggccgtcg agacctcgcc gtcctgtggg acatccgggt cgcgccggag gcgcgcgggc
     1621 ggggtgtcgg ctccgcgctc ttccgggccg tggaggcttg ggccgcggcg aggggctgtc
     1681 ggcaactgaa gatcgagacc cagaacatca acgtgccggc gtgcaggttt tatgcgcgtc
     1741 agggatgtgt cctcggggcc attaaccgct tcgcgtaccc tgagctaccg gacgaagtgc
     1801 agttgctctg gtacaagact ctctctcacc gcccgccgac cgggttagca acggcatgca
     1861 cccgcattca agcgttgact tgtcatcaga tttaacgtag aagcgccatg cactttcatc
     1921 tctcggacgc gccgtgcatc cgagcacatc gatgcggagg acatatggcc ggtgcgaaat
     1981 tagtggttct ctacccgttc cctcaagacg tggacgtttt cgagcgggct tacacggaag
     2041 agcatgtgcc gatggtgaac gaaaacacca tgaaaggcat ggcgaagttt gtcacgacga
     2101 aggtgttggg aacggccgac ggcagcgcgc ctcctttcta caggatggcc gagcttcatt
     2161 tcccgtccct tgccacgctc caggaatgcg cgtcttccgg gagcgcgcag gcggcggtgg
     2221 cgcatgcaat ctcgatttcc agcggaggga caccgctctt cctcgtcgcc gaggaggaga
     2281 ccacaacctt ctgaagttca gcctgtggcc agcaactaca gatgctaatg gacggccagc
     2341 ggcgtttacg aaatgaaggc gaagggctac cgaggcattc tgtggggcgg gctggcggcc
     2401 ggggtgctgg atttggcggc ggctctcgtc accagcagcc tgcgcggggt gacgccgact
     2461 cgcgtcctgc aagcgatagc cagcggggtg ctcggcgcgg actcttacag gggcggcgct
     2521 caaagcgcta cgctcggggt cgcgctgcac ttcgtgatcg cgttcggggc ggcggccgtc
     2581 tactacgcgg cgagccgcag gctcaagtgt ctggtgcggc ggcctgtcgt cagcggcttg
     2641 ctctacggcg tcgccgtcta cctctttatg aatctggtcg tgctgcccct gtcggcaatc
     2701 accttcaaag tctcttacgc acccgccgtg cttgcgacgg gccttgtcgt ccacatgctc
     2761 tgcgtcggtc tgcccatcgc gctcgccgtc cgccggttct cgaagtgacg aaatcctctg
     2821 tgtgagttgt cgcgttcaag cattctcgcc gacatggatt acctgataag gagagcgact
     2881 ctggccgacc gggccgcgat tgaacgactc atcgcggagt cggcgcgggg gctgagtcgg
     2941 ggcgactatt ccgagcggca gatcgaggcg gccgtggcgg ccgttttcgg cgttgacacc
     3001 agcctcatca tcgacgggac gtacttcgtc gccgagagcg gccgggagtt gatcggctgc
     3061 ggcgggtgga gcaggcgtaa aactcttttc ggcggcgacc agataccggg ccgcgacgcg
     3121 ggtgagcttg acccggagtc ggagccggcg aagattcgcg cgttcttcgt ccatccggcg
     3181 cacgcgcgaa gaggtgtggg gcgggcgatt ctcgcggcgt gcgagacgga ggcgcgctcc
     3241 ttcggctttc gttcgctgga gctgttggcg acgctccccg gcgtgcggct ttatgaagct
     3301 tgcgggtatg aggcgggcga gcacgtctgg catgaggctc aagggggcgt cccggtagag
     3361 tttgtgccga tgagaaagga cttgccgtag agcgagggcg accgggccgc aataccgggc
     3421 cgctaaaaat tgctgacaaa cccccgcccc cggcggcgca tccgactgca aggccaactt
     3481 ccccgtacaa cgtaccaagt gaaaggttga aacgtttgat gaaaaggact cttcacgtcg
     3541 ccatgctggc gctcgccgtc gcgtcggccg cgctcgcgtc cgacgcgctc gcgcagaaca
     3601 aacagacgcg ctctcagaac aacaacaccc cggccggcgc gcagccggcg ctcccgacgc
     3661 tcccggcctc cgacgccgtc gtgcttgtcg agttgcgccg actgatctcg gaggagctgc
     3721 cgcgcgcgct cgcctccgac gcgccccgtc tcgccgcggt caacgccgac atcgacgcct
     3781 tcaagacgag gaccggcatc gacccgcgcg cctttgatac gctggcggcc ggctcgcgct
     3841 tcatgtcgct cgcgggtggc aagacgaagc ttacgcacac ggtcgccgtc gcccgcggca
     3901 cgttcgacat ggcggcaatc gtggccgcgc tccgtgcgtc ctcgaagggc aatcaccgcg
     3961 agcagaagca cgcgggcaag agcgtccacg tcttcaccct caacgaggag gtcaagctct
     4021 tcgggctgtt gggcgtgcgc gtgagggatt tggccgtggc cgaactcgac cggacgacgc
     4081 tggccgtcgg ccacccggcc gccgtgctcg ccgccatcga cgccgcacgc ggacggggcg
     4141 cgctccgcgc ctccgacctc accgtgctca cgcagccgcg cgccgcctcg acgctcgtcg
     4201 ccttcggcgg caaagtctcg gcaacggcgt tcaaggacat cgatgtcggc acgcccgaga
     4261 tttcgcgcag cgtcgcctcc atccgtgagt tctacggcgc gctctcgacg accgccggcg
     4321 gctacgggat gcagacgacg ctccgcacga cgggcgccgc cgacgccaaa agcctcggcg
     4381 acaccatcgg cgccctcaag cagctcgccc cgctcttcat cgggcgtctg tcgggcgacc
     4441 ggcagcgcct cgcgcaatcg gccgtcgaga gcctacaagt gagcgcgcag ggcaacgagg
     4501 ttcgcctcag cctggagatc gcgcagggtg acatcacgac gctcgtgcgg acgttctagg
     4561 gcagactgaa aatatgtagg acagcattcc tgcctgtcct acatcaatcg ccgcacaaaa
     4621 aatgagaagg ccgcccgcga atgcgacggg cggccttctc atttttcgtc cgtgcggccg
     4681 gacggcttct actcgggaat gacggggaag ggcgcgctcg cgccgccgcc gagcggggag
     4741 cgcggcgcgg cgatgttcgg cgcttcgagg gccgccacct tcgcgagcac gtcgtcgtag
     4801 accttcacgt cgccctcgcg gtcgaggcgc gcgcgcaccg aggtcatgta gtcgtcgaag
     4861 acctgcatcc gtcgctcgtc gagggcgctc gcgatgagct tgtcgcgctc ggccccgaac
     4921 ttcgcgaggt cggcgtccgt gcgccccttg gcggcggcca ccacgtaggt ctgctcgatc
     4981 ttgatgggcg tcttggtgac gttgccggga cgcaaagcgt agatcgcctc gtcggccgcg
     5041 ccgctcgtgc cggccgcgcc gagcggcgtg cccagcgtgt actccttcga ctcctgcggc
     5101 ttcagcccca aacgttcggc gatggccttc agctcgtcgg gggtgttggc ggccgcaagc
     5161 tcacgcgccg ccgcctcaac ctgcgccgtg gccttctcgg ccttcacctt ctcggccacc
     5221 ttctccctga cctcggcgag ttccgggacg cgcgggtcgc gcttctccag aagcatgggg
     5281 atggcgaagc cgtcgcggat gctgacgcgc tcccccacgt cctgtgggtt gttgagcggg
     5341 gcgatggcgt cctcgaactg cggactgctg ccgatatcct tgacctcgtc gcccggcttg
     5401 acgaagcccg tctccctgac catctcggcc gcactcatgt tggcctcggc cgcgagttcc
     5461 tgcgccacct gctcgtggtt cttcgtctcc ttcagccgct cggccgcgcg ctgcgccacc
     5521 tgagcggcgg ccgcgtagcc gcgccggttg cgcaggctga cgagaagctc ctgcttcgcc
     5581 tcctcgaacg tcttatcgat ctgctcgccg cggcggaaga tgtaataggc gctggccttc
     5641 ttgtcgaaga aggggtcgct cacctgcccc gcctccaggc tgagggcgtt ctggtagatg
     5701 tcgccggtct tgtcggccgc cttcttgacg ggccggggaa gccagccgcc ggccttcgcc
     5761 gtcgccgggt cttcgctgcg gccgcgggcg agatcggcga acttctcttc ggacgccttg
     5821 aggtcgtcgc cgcggatgct ggccacaagc gccgtcgcct tgttgagcac ttcggtgtcc
     5881 agctccttcg ccgcgacctt caggacgatc tgctgcacgc gcacgcccgc caccttgttc
     5941 tcgggcttga gcgcgtcgaa ctcccggcgc agctcctcgt cgggaatctg cgcccgctcg
     6001 cccgccttcg attggctgac gaagacgtag cggatgttct tctgcggcac gtcgatcttg
     6061 aactcgtctt tgtgcgcgtc gtagtaggcg gcgagttccg cgtccgtggg cgtcagcgtc
     6121 cgggcgaggt cgctcgggag gacgggcacg tggagcagct cgaacttcgt gttctggcgc
     6181 tcccactccc gctgcacctc ctcctcggag acgctcgcgc cggccgtgac gaaggcgcgc
     6241 agcttctcct cggccagcgc gtcgcgaatc tgctgctcgg cgcgcttgac gctgccgtac
     6301 tgcgacagcg ccaactgctt gtaacgctcg gcgccgacga actgattcgt ctgcgggttg
     6361 gtgtagcgtt tgatgacggc gtcgcgcacc tcgccgtcgg agggggcgag gccgaggcgc
     6421 tcggcttcct gcgcgacgat gcgcccgcgg atcaggtcgt cgagcatccg gcggtcgccg
     6481 ccgaactgcg ccgggttgaa ctgagcgccg aacatcttcc ggtaacctga cttgaggtct
     6541 tcgagttccc cgaccgtgat cgagtcgccg cgcacgcggg ccaacacctc gcgggtcatg
     6601 ccgctggtgg agacggcgct gttttgcgac ggcgcgtaga agaagatgag gctgaggccc
     6661 atcaggagcg cgaaggcgat gatgacgtag ctgcgcgtcc tctccatccg gctgaacagc
     6721 ttgagcatgt ttacagtacc ttcctgaacg aacttcccgg cgtaggtttt ccgcggcgtg
     6781 tgggccttct cgaacgtcgc gggcgtcgta tagacgccct ccacgcggcc cctgcaaaac
     6841 gcggattgta gcggacgacg acgcgccgcc gcaaccgccc aatacagtag tcagtcgtta
     6901 gtcgtcagtt gtcagttgct tgtgtcgggc aagtcgttgc cgacaccgga aggctcaagg
     6961 ttgggaacgg aaacaactga ctaccgacaa ctgacgactg actaccattt gtgctaaatt
     7021 ttacgctcat ggatttccta tatatgactt ggtcgggtta cccaatctgg gcctggatgg
     7081 cgttcgccgt cttcatcctg ctgatgctgg ccctcgacct cggcgtcttg aaccgcaagg
     7141 cccacgccgt cacctaccgc gaggccgcga cgtggagcgc cgtctgggtg tcgctggcgc
     7201 tcgtcttcgc gggactggtc ttctggcaga acgggcggct cacgggggcc gagttcctga
     7261 ccggctacat catcgagctg tcgctctcgg tcgataatct cttcgtcttc ctgctcatct
     7321 tcggctactt caaggtgccg gccaagtacc agcaccgcgt cctcttctgg ggcgtgatgg
     7381 gcgcgctcat catgcgtatg acgatgatcg cgctcggcgc cgcgctcatc gagcgtttcc
     7441 actggatcat ctacgtcttc ggcgtcttcc tcgtctacac cggcatcaag atgatgcggc
     7501 aggaagagac ggccgccaac ccggaagata accccatcgt ccgcttcgtg tcgcggctcg
     7561 tcccgatgac gcgccactac gacggcgaga agttcttcac cgaagtcaac ggcaagcgga
     7621 tggggacgtt gatgctcctc gtcctcatca cggtcgaggt caccgacctc gtcttcgccg
     7681 tcgattccat cccggccatc ttcggcatca cgcagaacac cttcatcgtc tacacctcga
     7741 acgtcttcgc catcctcggc ctgcgctcgc tctacttcct cctcgccggc gtcatcgaga
     7801 agttccacta cctgaagatc ggcctctcgg tcgtgctcgc cttcgtcggc ctcaagatgc
     7861 tcaccgaggg cttcatcatc ggcaccacca tcggcggcta cacggtgcag aaggaggcgg
     7921 tcatcgtcgt ctcgctcgcc gtggtcgcgc tggtgctcat gagttcggtc gcggcctcct
     7981 acatctggcc caagagcgcc gagacgcacg ttgaagtgga gctgccgccc ggcttcggct
     8041 cgccttacga cgacgaccag ggtcagctcg gcggcgaaga ccgcccactg cctgcggcgg
     8101 aagtaaaaag tcaaaaggaa ataggcaaaa gcgccatcgc ggggccggag actgaatgac
     8161 cggcggccgt cccccgccgc cctgtgcctc attcactttt gccttttgcc ttttaccttt
     8221 ttacttcttg aacagtcgct ccgccgtgtg ttaagttcat ttatgcggtg gggccgagcg
     8281 gcttccgccg tgctttgact gcccctcccc gccccacgcg cgcgtcacgt cttctgaccg
     8341 aaggacgcgc ccggcggcgt gccccgaccg aagaatcctg agaacctgct tcatcccaat
     8401 tcggccgccg gtcccgaccc ttcatcccat atggcaacgc gcattagccg cttcgacctc
     8461 gagtggtgga tcgtctcgaa gcgacacatc tatctcgtca tcctcctcgt cgtcctttcg
     8521 ctcgcggcgg gcggggggag cgtctatctg tggttctacg gcaacccgtt caggggcgcc
     8581 gtcgagacgg acgccaaggc cgagggcgcg cgcttcgact ccttcgaggg cgacgtgcgc
     8641 gtgacgcgcg cctccacccg cgagaccttc ccggcccgct ccgagacgag cctctacccc
     8701 ggcgacatcg tccagacgca ggaggacggc cgcgcccgta tcacgctcgc cgacggctcg
     8761 acgctcctca tcaaacccaa cagcgtgatc accatcgccg agaacacgcg cagcgattcc
     8821 gacgggctgg ccaacgtccg cgtcgccgtt gatcgcggcc acgtcaacgt ccgcaccgac
     8881 cagcaggccg agaacgcctc gaacatcgtc aagacgccgc tcaccgagaa cgagctggcc
     8941 gcgcagaccg acgccagctt cggcgtgcgc gacgacaagc aggaggagat tcgcgtcgcc
     9001 accggctccg tcgagaccag cacgcgcaac ggcgagcggg ccacgttctc gaacggcgag
     9061 ttcgccctcg tcagccagtc ggccgacatc gtccagaagg aaaggctcat cgacgccccc
     9121 gcccccacgg ccccgcgcaa tctggagcgc atcgccgcgc gccgcggcgc cgcgacgagc
     9181 gtcgccctgc gctggcagaa gcccgcgaac ggcaacccca cccactaccg cgtcgaagtc
     9241 gccacctcgc ccttcttcgt cgatcccggc aaggtcatcg agcgcgacca actcccgacg
     9301 ctcaccttca ccgtcggcga ccttcagcag ggcaactact tctggcgggt gcgcgccgtc
     9361 gcgcagtcgg gtcaggcgag cgagtggagc gagccgcaga agttcgtcgt cgtccccgag
     9421 ggcggcggcg gcgaggcggc cacggtctcg aacgtgacgg tcgaatacgt cgccggcaac
     9481 gtctatatca tccgtgggca gacgcagccc ggcacgaacg tctactgcgc cggcaagcag
     9541 acgctcgccg gcagcggcgg caacttcgag ttgcagatcg ccgcccccca cggcgcccgc
     9601 gatgtcaccc tcgaagccgc cggcacgcag ggcgcccgcg gcagctaccg cgttgctttg
     9661 caacagtgat gagtgatgag tgataagtga tcagaaagaa aagaagctct aagctgttat
     9721 cgattagctc tcagccaaac agctaacagc taagagctaa cagcttctta tcacttatca
     9781 ctcatcaccc ctcgtatgaa acacgtcatc ctcatcggcg atgagcaggg gccgccggcc
     9841 ggcgcgctgg tcgagtcgtt gcgcgcggcc gggttcctgg ccgccgcgca ggcgttgcca
     9901 gccgcgggcg cagggtcttc cggcaacggc agcgacgagt cgtcatgccc gacggcggtg
     9961 ctcgttgagg tcgcctcggg cgccgacctg tcgcacgttc acgacgccgc gcagcgcgcc
    10021 tcggccgcct ggcccggctc gccactggtc gccttccgca gcgacaaggc gccgcagaac
    10081 ggccacgccc cgcacgcgca tctcgacacc tcgacgctcg aacgcctcgg cttccacgcc
    10141 gtggccgacg agccggcgca actcccggcc ctgctgcgcg cgctcgaatc gcgcggcctg
    10201 acgggcgagc tgcgcgcgcc cgagccggac gaacgtaccg cggcgcgcgc cgccgagacg
    10261 ccccctgcga gcctgctgct gcccgagcgc ctcggcgtcg agcaactgcg cgtcgccttc
    10321 gagacggtcg cctcgctcca cttctcaaat gaccagcgtt cggcggcgtc gtccgcgctc
    10381 gtcggcctcg ccgcgctcgt cgcggccgaa cg
//
LOCUS       DASVES010000163         8017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_992671, whole genome shotgun sequence.
ACCESSION   DASVES010000163 DASVES010000000
VERSION     DASVES010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992671"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1554)
                     /locus_tag="VF240_06615"
     CDS             complement(124..1554)
                     /locus_tag="VF240_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7174936.1"
                     /translation="MLHIPLLRKGEPYRSLEVTRVADSRKREPFVEVSQANVGLIRRD
                     LLDQSSPRRALQQFTTAELLDICTRAAGHFTEDALSVGDEEQTPEDYVRQLSATTGLP
                     HALARRNMERVRGVMTRMENILGGLTRGLDLSILDRGAGHSAGHALSFFARGDSLGVV
                     LPSNSPGVHALWVPAVPLKVPLVLKPGGSEPWTPYRIIQSFIRAGAPPAAFSYYPTDH
                     VGAAEILRRTGRGMVFGDSSTTSVWKADPRIEIHGPGHSKIVIGDDLVDRWEDFLDVM
                     VASIADNGGRSCVNASGVWVTRHADKIAEALAERLSQIVPRAVDDPAATLAPFADPRV
                     AARVSQMIDAALDSEPGARDVTAARRGPERLAELDGGTYLLPTVVHCESAAHPLANRE
                     YMFPFASVVQVRPEEIPDALGFSLVVTAITEDERLIERLLASPLVDRLNVGPVSTMQI
                     AWDQPHEGNLFEHLYARRAFQRAAAL"
     gene            complement(1661..2908)
                     /locus_tag="VF240_06620"
     CDS             complement(1661..2908)
                     /locus_tag="VF240_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7174937.1"
                     /translation="MMRVKLRPTTLFLFSLVVCGAQSAGAQEWAQWRGAARDGVVKTF
                     AAPAAWPKELKRNWKVPTGGGYSSPLVSGGRVFLHTRQEEQEVVSAFDLATGKTIWSK
                     PYPAPFSKNQYAIKMGKGPNSTPVLHAGNLYTLGVGAILSAFDAKTGELKWRKDFSAQ
                     IDTSKMFCGTAMSPVVEKGLLIVHVGDDRGGQVVAFDAATGSEKWKWQGDGPGYSSPI
                     VVDLAGARQVVTLTDKSVVGLAADTGKLLWKTAFTDEWNENIVTPILYGKTLIVSGIR
                     QGTKSFEIVKTAAGHEAKQLWHNPQVAMYMSSPVLDGDYLYGLSSQRKGQYFCLDAKT
                     GQIVWATEGRDGNQAAVLSAGGVLLFLTADADLIVAAKSRKGFEPLARYRVADAATWA
                     HPAVFGKQILVKDEAALSLWSVE"
     gene            complement(3120..4145)
                     /locus_tag="VF240_06625"
     CDS             complement(3120..4145)
                     /locus_tag="VF240_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174938.1"
                     /translation="MREVVAWHFDPETGSPFWLEYAKGLDFDPRREIQTYDDLARLGP
                     FQDEWLRGGPVRRWVPRGLADRPICVFETGGSTGVPKSRINIDDFRIDYSLFSDTLPD
                     EYFPKGADWLSLGPTGPRRLRLAVEHLAQHRGGICFMVDLDPRWVIKLLKMGESRMAE
                     AYKQHVVEQGLTLLRAHPDIHCLFTTPKLLEALCEKVNLKRMGVTGVFCGGTEMTPQF
                     HRFAVEELLGPDVYFAPTYGNTLMGLAVHKPRAVGDPFDIIYYPPAPRAMIEVVDPDD
                     PDRLVGYGETGRVRLTTLTKEFFMPRFLERDEAEREPPCGAYPWDGVRNVRPFSRLQT
                     SVVEGVY"
     gene            4702..5886
                     /locus_tag="VF240_06630"
     CDS             4702..5886
                     /locus_tag="VF240_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7174939.1"
                     /translation="METTGHRESPPPAWASERLQLSLAYFLYFVTLGIVFPYFPAYLA
                     ARGLDAVAIGWVMALGPLARIVVPPLLGLAADRRRGPRFWSVVAAWGALSGMLLMWAP
                     GGLPLLYAGAVVYSLFNAPSMPLLDAMTLRRATARFGHVRMWGSVGYVLTSFALGTLF
                     PSLPPEVIITSCVGSLLLFSIFLSASRIEDAPTPRTDWGELPRVLRGRAVWLLLGALL
                     LSRMAAGPLSGFYIIFVNEQGLGGDVVALTWGIAIATEVCVMLAVDRLINRFGAGRVL
                     AAGVLLDALRWFFYSVIATKAALLAAAPAHGLAFAMLYVAGVRALTDIIPREFRALGQ
                     GLGAAAQALGLAAGLILAGYLYKTSGSRVMFAAAGIVGLASTVCALAFGQLHGRSTRL
                     QN"
     gene            complement(5973..7505)
                     /locus_tag="VF240_06635"
     CDS             complement(5973..7505)
                     /locus_tag="VF240_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEX7174940.1"
                     /translation="MRTRTPVILSLLLLLATAPAASAQTEQWVPYTLRSDQTAVRVWT
                     SGERTHARVNIVFNDGGYRVTDIGPVVRQGNNVSVDFIIDRWTGGATQALVYKEYFFD
                     LGALPAEPGTFTFTVKSRGADVRGVTFDPRQIVEHWEEASLERNAVGFTVWTTGGVTF
                     TGIGLGFPDDGYRVVAWQPPARSGNDFVSQIRLERWTGRAETRQFQGDRRVFVLGQLP
                     PTETFTVSAQFSDGVRHTSAPFTPAGTSRPAGSNAIDDPAFFVRQHYLDFFGREPDPA
                     GLNFWYNELTNCVPFTTECVERRRESVSAAFYLSVEFQQTGFVVHRLYKAAFNRIPRF
                     AEFLADTRRIGEGVVVNGEGWQERLEANTRQFVEEFAARAAFRERYPEAMTPEQYVDI
                     LNLNIRHDFPELDPGPLTPAEVAQLVEGLRSGAETRGAVLRKVANHPHLVQQEFNRAF
                     VLSEYFGYLRRNPDDAPDNNWEGYFFWLAKLNSHRGDFQSAQMVRAFLVSGEYRNRFS
                     PN"
     gene            complement(7658..>8017)
                     /locus_tag="VF240_06640"
     CDS             complement(7658..>8017)
                     /locus_tag="VF240_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174941.1"
                     /translation="EAPGGRRLNRAAFVQVFGRQGTLGRNAVRGFGFSQIDLALRREF
                     RLSERARLQLRAEVFNLFNRPSFGDPVADLANPRFGLSTQTLARSLGAGGANGGLSPL
                     YQVGGPRSIQLAVKVLF"
BASE COUNT         1290 a         2603 c         2732 g         1392 t
ORIGIN      
        1 atgtagagga gcttcatctc gaactcaagg cgacccgcga cgccctccct gacggtcggg
       61 ctactgccat agatgacgcg cgggggcagt agcccgaccg tcagggaggg cgtcgcgcac
      121 gggctacaaa gcggcggcgc gctggaaggc gcggcgggcg taaaggtgct cgaagaggtt
      181 gccctcgtgc ggctgatccc aggcgatctg catcgtggag acggggccga cgttgagtcg
      241 atccacgagc ggggaggcca gcagccgttc gatcagtcgc tcgtcctccg tgatcgccgt
      301 gacgacgagg ctgaagccta aagcgtcggg tatctcctcg ggtcgcacct ggacgacgct
      361 ggcgaacggg aacatgtact cgcgatttgc cagcgggtgc gcggccgatt cgcagtggac
      421 gaccgtcggc agcagatagg ttccgccgtc gagttcggcc agtcgctcgg ggccgcgtcg
      481 ggccgccgtc acgtcgcgcg cgcccggctc ggagtcgagc gccgcgtcga tcatctgcga
      541 gacgcgcgcg gccacgcgcg ggtcggcgaa gggcgcgagc gtcgccgccg ggtcgtccac
      601 cgcgcgcggc acaatctgtg agagtctttc ggccagcgcc tcggcgattt tgtcggcgtg
      661 gcgcgtcacc cagacgccgg aggcgttgac gcaggagcgc ccgccgttgt cggcgatgga
      721 ggcgaccatc acgtcgagga agtcttccca gcggtcaacg aggtcgtcgc cgatgacgat
      781 cttgctgtgg ccggggccgt gaatctcgat gcgcgggtct gccttccaga ccgaggtcgt
      841 cgaagagtcg ccgaagacca tgccgcgccc cgtgcggcgg aggatttccg cggcgccgac
      901 gtggtcggtc gggtaatagc tgaaggccgc gggcggcgcg ccggcgcgga tgaacgactg
      961 gatgatgcga tagggcgtcc acggctctga gccgccgggc ttgagcacga gcggaacttt
     1021 gagcggcacg gccggcaccc acagggcgtg gacgccgggc gagttgctcg gcagcacgac
     1081 gccgagcgag tcgccgcgcg cgaagaagct cagcgcgtgc ccggccgagt ggcccgcgcc
     1141 gcggtcgagg atggagaggt cgaggccgcg cgttaagccg ccgaggatgt tctccatccg
     1201 cgtcatcacg ccgcgcacgc gctccatgtt gcggcgcgcg agcgcgtgcg gcagccccgt
     1261 ggtcgccgac aactgccgga cgtaatcctc cggcgtctgc tcctcgtcgc ccacgctcag
     1321 cgcgtcctcc gtgaagtgcc cggcggcgcg cgtgcagatg tccaacaact cggcggtggt
     1381 gaactgctgg agcgcgcggc gcggggacga ctgatcgagc aggtcgcggc ggatgaggcc
     1441 gacgttagcc tgactgacct cgacgaacgg ctcacgcttg cggctgtccg cgacgcgcgt
     1501 cacttccaga ctccggtacg gctcgccctt acgcagcagt ggtatgtgta gcattacgat
     1561 aacagggagt caggagtcag gagtcagaag acagaataaa agcgctgccg cccactgtct
     1621 tcttccttct gactcctgac tcctgactcc tgactccttc tcattccacg ctccacaaag
     1681 agagcgccgc ctcgtccttc acgagaatct gtttaccgaa gacggccggg tgcgcccacg
     1741 tcgcggcgtc ggccacgcgg tagcgcgcca gcggctcgaa gcccttgcgg cttttggcag
     1801 cgacgatgag gtcagcatcc gccgtgagga acaacagcac gccgccggcg ctcaggaccg
     1861 ccgcctgatt gccgtcgcgc ccttcagtcg cccagacgat ctggcccgtc ttcgcgtcga
     1921 ggcaaaagta ttgccccttg cgctgcgaag acaggccgta gagataatcg ccgtcgagca
     1981 cgggcgaact catgtacatc gccacctgcg gattgtgcca gagctgtttg gcctcgtgcc
     2041 cggcggccgt cttcacgatc tcgaacgact tcgtcccctg cctgatgccg gagacgatga
     2101 gcgtcttgcc gtagagaatc ggcgtcacga tgttctcgtt ccactcgtcg gtgaaggccg
     2161 tcttccacag gagcttcccc gtgtcggcgg cgaggccgac gaccgacttg tccgtcagcg
     2221 tgacgacctg acgcgcgccc gccagatcga cgacgatggg cgaggagtag ccggggccgt
     2281 cgccctgcca cttccacttc tcactgcccg tcgcggcgtc gaaggcgacg acctgcccgc
     2341 cgcggtcgtc gccgacgtga actatcaaaa gtcccttctc gacgaccggc gacatcgccg
     2401 tgccgcagaa catcttcgag gtgtcgatct gcgcggaaaa atctttgcgc cacttcagct
     2461 cgccggtctt cgcgtcgaag gcggagagaa tggcgccgac gccgagcgtg tagaggttgc
     2521 cggcgtgcaa aacgggcgtc gagttcggcc ccttgcccat cttgatggcg tactggttct
     2581 tgctaaacgg cgccgggtag ggcttcgacc agatggtctt gccggtggcg aggtcgaagg
     2641 ccgagacgac ctcctgctcc tcctgtcgcg tgtgcaggaa gacgcgcccg cccgagacga
     2701 gcggcgacga gtagccgccg cccgtcggca ccttccaatt ccgtttcaac tccttcggcc
     2761 acgcggccgg agcggcgaaa gtcttcacca cgccatcgcg cgccgcgccg cgccactgcg
     2821 cccactcctg cgcccccgcg ctctgcgcgc cgcacacgac gagcgagaac aggaagaggg
     2881 tcgtgggccg gagtttcact ctcatcatgg ctggtaacac ctttcgcaat ggagaagacc
     2941 tagccccgtg cttcgcttac gggatacttg aatggcagca actcatgaac tcaccgcaga
     3001 ggcgcggagg gcgcagagga cgcccagagg gttgagactg ttcaatcctc cgcgccccct
     3061 ccgcgtcctc tgcgcctctg cggtgaataa actatatccc atgctcaagt gaaatttctt
     3121 tagtacacgc cttcgaccac tgacgtttgc aggcgcgaga acgggcggac gttacgcacg
     3181 ccgtcccacg ggtacgctcc gcagggcggc tcgcgctcgg cctcgtcgcg ctccagaaag
     3241 cgcggcatga agaactcttt ggtcaaagtc gtcagacgca cgcgccccgt ctcgccgtag
     3301 cccaccaagc ggtcggggtc gtccgggtca acgacctcga tcatcgcgcg cggcgcgggc
     3361 gggtagtaga tgatgtcgaa ggggtcgccc accgcgcgcg gcttgtggac ggcgagtccc
     3421 atcagggtgt tgccgtaggt gggcgcgaag taaacgtcgg ggccgagcag ctcctcgacg
     3481 gcgaagcggt ggaactgcgg cgtcatctcg gtgccgccgc agaagacgcc ggtgacgccc
     3541 atgcgcttga ggttgacctt ctcgcagagg gcttcgagga gcttcggcgt cgtgaacagg
     3601 caatggatgt cggggtgcgc gcgcagaagc gtcaggccct gctcgacgac gtgctgcttg
     3661 taggcttcgg ccatccgcga ctcgcccatc ttgagcagct tgatgaccca gcgcgggtcg
     3721 agatcgacca tgaagcagat gccgccgcga tgttgcgcga ggtgctcgac ggcgagacgc
     3781 aaacggcgcg gccccgtggg gcctaaggaa agccagtcgg cgcctttggg aaaatactcg
     3841 tcggggagcg tgtcgctaaa gagcgagtag tcgatgcgga agtcgtcgat gttaatgcgc
     3901 gacttgggaa cgcccgttga gccgccggtc tcgaagacgc agatggggcg gtcggcgaga
     3961 ccgcgcggca cccagcgacg cacggggccg ccgcgcagcc actcgtcctg aaaggggccg
     4021 aggcgcgcga ggtcgtcgta ggtctgaatc tcgcggcgcg ggtcgaagtc gagaccctta
     4081 gcgtattcga gccagaaggg ggagccggtc tcggggtcga agtgccacgc aactacttcg
     4141 cgcacccacg cgtcgagcgt ctcacgcgcc gcgcgcgcgt cctgttggat tgacgtttcg
     4201 gtttgaatga ctcggcgcct ccttttagcg tggagattac ttaactgcga accgttagca
     4261 caaccgcgcg acgcggaacc gcgcggcgac gatgtaaaat tatatttaca aacgccggga
     4321 aagactattc tctgcgggtc gcgcgcggcg agaggtaaaa gttaggaagc caagtcgcag
     4381 gcatcaagga gccgcggagc ggcggaagat tgtagccccg gtcgtgagac cggggtcagc
     4441 gatttagaag aaacaaggaa gccccggagg ggcgaaagaa ccgcaacgtt gagcacgatt
     4501 ctcgtcgcgg ttctttcgcc cctccggggc tttcaaatca ttatccggcg tgtccccacg
     4561 gctggcgccg tgggctacat tcttccgccg catcctgcgg ctccttgatg cctgcgatga
     4621 cgcccgctat tcgtcctcac cacttttgcc ttttgccttt ttacttttgc cttacctgtg
     4681 cccgcgtgat agcttcgcgc gttggaaacg accgggcacc gcgaatcccc tccccccgcg
     4741 tgggcctccg aacgcctgca actgtcgctc gcctacttcc tctacttcgt cacgctcggc
     4801 atcgtcttcc cttacttccc cgcctacttg gcggcgcgcg gcctcgacgc ggtcgctatc
     4861 ggctgggtga tggcgttggg gcctctggcg cgcatcgtcg tgccgccgct gcttggactc
     4921 gcggccgacc gccgccgggg gccgcgcttc tggagtgtgg tcgcggcgtg gggtgcgctc
     4981 tccgggatgc ttctgatgtg ggcgccgggc ggattgccgc tcctctacgc gggcgccgtc
     5041 gtctactcgc tcttcaacgc gccgtcgatg ccgctcctcg acgcgatgac tctgcgccgc
     5101 gccacggccc ggttcggaca cgtccgcatg tggggcagcg tcggctacgt cctgacgagc
     5161 ttcgcgctgg ggacgctctt cccgtcgctg ccgcccgaag tcatcatcac gagctgtgtc
     5221 ggctcgctgc tgctgttctc gatcttcctc agtgcgtcgc gcatcgagga cgcgccgacg
     5281 ccgcgcacgg actggggcga gttgccgcgc gtgctgcggg gccgcgcggt gtggctgctg
     5341 ctgggggcgc tgctcctgag ccgcatggcg gcggggcctc tgtccggctt ctacatcatc
     5401 ttcgtcaacg agcagggtct gggcggcgac gtggtcgcgc tcacgtgggg catcgccatc
     5461 gcgaccgagg tctgcgtgat gctcgccgtt gaccgcctca tcaatcgctt cggggcgggc
     5521 cgcgtcctcg ccgcgggcgt gctgctcgat gccctgcgct ggttcttcta ctcggtcatc
     5581 gccacgaagg cggcgctgct cgccgccgcg cccgcgcacg gcctcgcctt cgccatgctc
     5641 tacgtggcgg gcgtgcgcgc actcacggac atcatcccgc gagagttccg cgcgctcggg
     5701 caggggctag gcgcggcggc gcaggcgctc ggtctggccg ccggactcat cctcgccggc
     5761 tacctctata agacttccgg cagccgcgtg atgttcgcgg ctgccggaat cgtgggcctc
     5821 gcctcgacgg tgtgcgccct cgccttcggc caactccacg gccgctcgac gcgcttacaa
     5881 aactaaggga acatggataa gacggatgaa gcggatgagc acggatttga tccgtcctta
     5941 tccgtctcat ccgtgttccc tgcccggcgt cgttaattcg gcgagaagcg gttgcggtac
     6001 tcgcccgaga cgaggaacgc cctgaccatc tgtgcggatt ggaagtcccc gcggtggctg
     6061 ttgagcttcg cgagccagaa gaagtagccc tcccagttgt tgtcgggcgc gtcgtcgggg
     6121 ttgcgtcgca ggtagccgaa gtactccgac aggacgaagg cgcggttgaa ctcctgctgc
     6181 acgaggtgcg ggtggttggc gaccttacgc aggacggcgc cgcgcgtctc ggcgccggag
     6241 cgcaggccct cgacgagctg cgcgacctcg gccggcgtga gcggccccgg atcaagctcg
     6301 gggaagtcgt gccggatgtt cagattcaaa atgtccacgt actgctcggg cgtcatggct
     6361 tcggggtatc gctcgcggaa cgcggcgcgc gccgcgaact cttcgacgaa ctgccgcgtg
     6421 ttcgcctcca gtcgctcctg ccagccctcc ccgttgacga cgacaccctc gccgatccgg
     6481 cgcgtgtcgg ccaggaactc ggcgaagcgc gggatgcggt tgaaggcggc cttgtagagc
     6541 cggtggacga cgaagcccgt ctgctggaac tcgaccgaga ggtagaaggc cgccgagacg
     6601 ctctcgcgcc ggcgctcgac gcactcggtc gtgaagggca cgcagttcgt cagctcgttg
     6661 taccagaagt tcagccccgc cgggtcaggc tcgcggccga agaagtcgag atagtgctgg
     6721 cggacgaaga aggccgggtc gtcgattgcg ttgctgccgg cgggtcggct cgtccccgcg
     6781 ggcgtgaacg gcgcgctcgt gtgcctgacg ccgtcggaga actgcgcgct gacggtgaag
     6841 gtctcagtgg gcggcaactg tccgagcacg aagacgcgcc tgtcgccctg gaactgtcgc
     6901 gtctcggcgc ggcccgtcca gcgttcgagc ctgatctgcg agacgaagtc gttgcccgag
     6961 cgcgcgggcg gctgccaagc gacgacgcgg tagccgtcgt cggggaagcc gagtccgatg
     7021 ccggtgaagg tcacgccgcc cgtcgtccag acggtgaagc cgacggcgtt tctttcgagc
     7081 gacgcttctt cccagtgctc gacgatctgg cgcgggtcga aggtgacgcc gcgcacgtcc
     7141 gcgccgcgcg acttgaccgt gaaggtgaac gtgcccggct cggcgggcag cgcgccgagg
     7201 tcgaagaagt attccttgta gacgagcgcc tgagtcgcgc cgcccgtcca acggtcgatg
     7261 atgaaatcga ccgagacgtt gttgccctgc cgcacgacgg ggccgatatc cgtgacgcgg
     7321 tagccgccgt cgttgaagac gatgttgacg cgcgcgtgcg tccgctcgcc ggaagtccag
     7381 acgcgcacgg ccgtctggtc ggagcgcagc gtgtaaggca cccactgctc ggtctgcgcc
     7441 gaggccgcgg gcgcggtcgc gagtagtagc agaagggaga ggatgacagg cgtgcgagta
     7501 cgcatgagta acactccttt aatcgtcaat cgtaaatcgt aaatcgtgaa tcgtggttgc
     7561 cgcacatccg gcccgagtgg ttaatcgaaa gctgagcgat gctcaagcca catcgattga
     7621 cgatttacga ttcacgattt acggcctttc ttctcgttca gaaaagaacc ttcaccgcga
     7681 gttgaatcga gcgcgggccg ccgacctgat agagcgggga gagtccgccg ttggcgccgc
     7741 ccgcgccgag gctacgggcg agcgtctgcg tgctcaggcc gaagcgcggg ttggcgaggt
     7801 cggcgacggg gtcgccgaag ctcgggcggt tgaacaggtt gaagacctcg gcgcgcagtt
     7861 ggagacgcgc gcgctccgag agccggaact cgcgccggag cgccagatcg atttgcgaga
     7921 agccaaagcc gcgcacggcg ttgcggccga gcgtcccctg ccgcccaaag acctgcacga
     7981 aggccgcgcg gttcagacgc cgcccgccgg gcgcttc
//
LOCUS       DASVES010000164         9408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1370159, whole genome shotgun sequence.
ACCESSION   DASVES010000164 DASVES010000000
VERSION     DASVES010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370159"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..168)
                     /locus_tag="VF240_06645"
     tRNA            complement(96..168)
                     /locus_tag="VF240_06645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Lys,seq:ttt)
     gene            complement(261..563)
                     /locus_tag="VF240_06650"
     CDS             complement(261..563)
                     /locus_tag="VF240_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174942.1"
                     /translation="MPEVFVLPYHLVDHFTNPGLDADAGIIPMNQYMSKTRKNGGNDN
                     CHDLFKNLRWMKDAAIVLEAVLLRSIFIEVAGRFDRSTSGATFCEFAHLNVPVPSQ"
     gene            complement(708..2027)
                     /locus_tag="VF240_06655"
     CDS             complement(708..2027)
                     /locus_tag="VF240_06655"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX7174943.1"
                     /translation="MSRIIVVTGAQWGDEGKGKIVDLLAGRFDVVARFQGGHNAGHSV
                     QVGERSFALHLVPSGIVHDGVMCVLGNGMVIDPKAFFEEADRLASQGVEVTPERVMVS
                     SRAHLILPYHRALDHTSEERLGNERVGTTLRGIGPAYEDKAGRRGIRVADAANPEVLR
                     KRIERNLEDANHIIRAYGGQPLDPDTVFDEFYPHAERLAPYIADTAHYLNRAAREGRS
                     ILVEGAQAALLDVDHGTYPFVTSSSTTSGGVSVGLGIPPHRITGVLGIVRTYTTRVGE
                     GPFPTEMLEGEAELGQLIRERGREYGVSTGRPRRCGWFDAFATNYAAEINGFDSVALT
                     KLDVLDALDHIKVCVGYRLDGRDLETFPPVSHDLRRVEPVYETLPGWNTSTVGATDFD
                     ALPPRAQDYVRFLSERIGVEIGLISTGPERDQTVVCQDSALAGWLKE"
     gene            complement(2113..2520)
                     /locus_tag="VF240_06660"
     CDS             complement(2113..2520)
                     /locus_tag="VF240_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7174944.1"
                     /translation="MKPSHSVRIRAAQRRTFEERLAAKRTRAVEEGKFRARQVQGKLR
                     RFVNIRFRKEEVDEALELRRGDCNRCGACCEILFKCPFLKKHDDFTTTCGIYEDRPDQ
                     CRLYPIERRDLDEVRGQCSFYFIERPIKLEKAS"
     gene            complement(2680..3027)
                     /gene="trxA"
                     /locus_tag="VF240_06665"
     CDS             complement(2680..3027)
                     /gene="trxA"
                     /locus_tag="VF240_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7174945.1"
                     /translation="MSEHVNDVSDTNFESDVLKSDKPVLVDFWAAWCAPCRMLAPTVE
                     AVAEKFAQGARVVKLNVDDNPATSQRYGIKGIPTLILFKNGKEEERVVGATSKEAISR
                     MIEKHIGGAVGAG"
     gene            complement(3154..4587)
                     /locus_tag="VF240_06670"
     CDS             complement(3154..4587)
                     /locus_tag="VF240_06670"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX7174946.1"
                     /translation="MRVAFLSSEAVPYSKTGGLGDVSGALPKALIETGEADVTLVTPL
                     YGETKRDLLSAQPIFDNLDVEWLGRIRRTWVWYSEAAGAPTFLIDAPEYFMRATIYGH
                     RDDHERFAFFSRAALTLLGRLGEAPDIVHMNDWQCGFASAWIRAGRPWGGYLSETRTL
                     LSIHNLAFQGMFDPTELWRLGFRPGEETNVFLSNNVASAMKAGLMTADALSTVSRRYA
                     QEIQTREHGYGLEWLLRQRRDRLIGITNGVDYEVWNPGTDPHLAAHYSAADLSGKREC
                     KRDLLRRFGLPEEELHRPIVATVSRLTPQKGFDLIKQAAGRLLETGAFLVFLGSGSRE
                     YEDFLQALHEHAPRRVGVYRGFNEPLAHQIEAGADIFLMPSQYEPCGLNQMYSMRYGT
                     VPVVRATGGLDDTVEHFDRSRGAGAGFKFGPYTADAMMGALHEALHCYGQPALWRHVQ
                     LNGMREDNSWSAAARRYLEIYRRVASL"
     gene            complement(4758..6263)
                     /gene="rho"
                     /locus_tag="VF240_06675"
     CDS             complement(4758..6263)
                     /gene="rho"
                     /locus_tag="VF240_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX7174947.1"
                     /translation="MKSRRCPLGRGGAHKRRTEMSTRRTRTIKSSDNANGADADAAVK
                     ATAAIEPPPPPPPDVDIGTDREGEGGEGDGDDGIGFVFDGSTFNLATLKEMSISKLTQ
                     IAKDLDVPGATGMRKQELIFKVLAAQTEKSGLIFSEGVLETLPDGFGFLRAPEYNYLP
                     GPDDIYVSPSQIRKFDLRTGDTVSGQIRPPKEGERYFALIKVEAINFEAPGETRDKVF
                     FDNLTPLYPESQLRMETNADHIVGRVIDLVTPLGKGQRALIVAPPRTGKTMMLQAIAN
                     SVSQNHPEVSLIVLLIDERPEEVTDMQRSVQGEVISSTFDEPPTRHVQVADMVIEKAK
                     RLVEHKRDVVILLDSLTRLARAHNAVVPPSGKILSGGIDANALQRPKRFFGAARNIEE
                     GGSLTIIATALIDTGSRMDDVIFEEFKGTGNSEIHLDRRLSDKRLFPAIDLQRSGTRK
                     EELLISKEDLNRIWVMRRVLNPLSPVEQMEVVLERLGKTKTNSEFLASMQT"
     gene            complement(6596..7207)
                     /gene="yihA"
                     /locus_tag="VF240_06680"
     CDS             complement(6596..7207)
                     /gene="yihA"
                     /locus_tag="VF240_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX7174948.1"
                     /translation="MKVASADFVKSAFEESQWPRDSLPEVAFLGRSNVGKSSLMNSLL
                     GVKGLARTSSTPGRTQSLNYFLINRRFYFVDVPGYGYARVPQEVRREWGHLVTEYLAK
                     RTSLVLSIHIVDSRHDPTKLDLQLREWLEFHGRASVTVATKSDKLSANELRKSLARAH
                     RILGVGDDGASEIVAYSAVTGRGRESVWRAIEEALAASKIPTS"
     gene            complement(7428..>9408)
                     /gene="lon"
                     /locus_tag="VF240_06685"
     CDS             complement(7428..>9408)
                     /gene="lon"
                     /locus_tag="VF240_06685"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7174949.1"
                     /translation="QTDALQTALRNQDSSHLADALASQIKISVEDKQGLLEIFSTTGR
                     LQRLVELLEVEVDKRQLDRTIQARVKRQMERAQKEYYLNEQIKAIHKELGRKDEKAEL
                     EDLRKRIDEAGMTEEAKEKALQELHRLEAMPPMSAEGTVSRTYIDWLLSVPWKQKTKD
                     LKDLSRAEDILNEDHFGLEKVKERILEYLAVRQLVKNPKGSILCFVGPPGVGKTSLGK
                     SIARATGRKFVRLSLGGVRDEAEVRGHRRTYIGALPGQIIQMMKKAGTVNPLILLDEV
                     DKLGADFRGDPSAALLEVLDPEQNNTFQDHYLDVEYDLSKVFFIATANVLHTIPPALQ
                     DRLEIIRLSGYTEREKLQIAKRHLVVKQAEGSGIKPEQVDFTDEGILEIIRHYTREAG
                     VRNLEREVGSCMRKTARKLVGESHGAEFREVLTAERVREMLGPIKFRQLTAHATSEIG
                     LAVGLAWTEVGGEVLQIEATLTNGRGGVRLTGKLGEVMQESAQAALTCIKARAERLGV
                     NLDFIRKRDLHIHIPEGAIPKDGPSAGVTIATAMASALMRVPVRNDVTMTGEITLRGK
                     VLPVGGIKEKLLAAHRFGITSVLIPKDNEKDLLEVPEEVRSELRVQLVETIDEVIALA
                     LEEACPTSAGEGSGAEHPPVWTTDTPTQGTPTMAD"
BASE COUNT         1584 a         2887 c         3149 g         1788 t
ORIGIN      
        1 ctaaaatcag gcacgttcgc gggaggtcct actgacgggc gcaccagccg tgccagggag
       61 gagttaaacc ctggttcgcg ccgggtgaaa tgagatgagc cgagccggac tcgaaccggc
      121 gacccgctgg ttaaaagcca gctgctctac cagctgagct atcggcccgt gaattaaaag
      181 tgattatagt ggcagtcaac ttattattca acaacgtgtc ataggcggct tataatgcgg
      241 gcgctgtttt cgttctaacc tcactgtgaa ggaacgggga cgttgagatg cgcgaactcg
      301 caaaacgttg cgccagatgt ggatcggtca aagcggccgg cgacttcaat aaaaatagat
      361 cgaagaagga cggcctccag cactattgcc gcgtccttca tccagcggag gttcttgaag
      421 aggtcgtgac agttgtcatt gccgccgttc ttcctggtct tggacatgta ctgattcatg
      481 ggaatgattc ccgcgtcggc gtcaagtccg gggttcgtga agtggtcaac gaggtgatag
      541 ggcaggacga agacttccgg catggcacaa cctcctcaag ctgggtgacg atatcatcgc
      601 cgacaagcaa ggggcggcga tctctcatgt tgcgcgaggg atgagccggg cgggattcga
      661 acctgcggcc cgccggttaa aagccggccg cttcaaccgt cgaaagatta ttctttaagc
      721 caaccggcga gcgccgagtc ctggcagacg accgtctggt cgcgctcggg gccggtggag
      781 atgaggccga tctcgacgcc gatgcgctcg gagaggaagc ggacgtaatc ctgtgcgcgg
      841 ggcgggagcg cgtcgaagtc ggtggcgccg acggtcgagg tgttccagcc cggtagggtt
      901 tcgtagaccg gctcgacgcg gcgcaggtcg tgcgagacgg gggggaaggt ttcgaggtcg
      961 cggccgtcga gacggtagcc gacgcagacc ttgatgtggt cgagggcgtc gagcacgtcg
     1021 agcttggtca gcgccacgga gtcgaagccg ttgatctcgg cggcgtagtt ggtggcgaag
     1081 gcgtcgaacc agccgcagcg gcggggccgt cccgtggaga cgccgtactc gcgcccgcgc
     1141 tcgcggatga gctggcccaa ctcggcctcg ccttccaaca tttcggtcgg gaaggggcct
     1201 tcgccgacgc gcgtcgtgta ggtgcggacg atgccgagca cgcccgtgat gcggtgcggc
     1261 ggaatgccga gaccgacgga gacgccgccc gaggtcgtcg aagaagaagt gacgaacggg
     1321 taggtgccgt ggtcaacgtc taagagcgcg gcctgcgcgc cctcgacgag gatcgagcgc
     1381 ccttcgcgcg cggcgcggtt gaggtagtgc gccgtgtcgg cgatgtaggg cgcgaggcgt
     1441 tcggcgtgtg ggtagaactc gtcgaagacc gtgtcggggt cgagcggctg gccgccgtag
     1501 gcgcggatga tgtggttggc gtcttcgagg ttgcgctcga tgcgcttgcg caggacttcc
     1561 gggttggcgg cgtcggccac gcggatgccg cgccggcccg ccttatcctc gtaggccggg
     1621 ccgatgccgc gcagggtcgt ccccacgcgc tcgttgccga ggcgctcctc gctcgtgtgg
     1681 tcgagcgcgc ggtgataggg gaggatgagg tgcgcgcgcg agctgaccat gacgcgctcg
     1741 ggtgtgactt ccacgccctg tgaggcgaga cggtcggcct cctcgaagaa ggccttcggg
     1801 tcgatgacca tcccgttgcc gagcacgcac atcaccccgt cgtggacgat gcccgacggg
     1861 acgaggtgga gcgcgaaaga gcgttcgccc acctgcaccg agtgccccgc gttgtgcccg
     1921 ccctggaagc gggccaccac gtcgaagcgc ccggccagca gatcgacgat cttgcccttg
     1981 ccctcgtcgc cccactgcgc gcccgtgaca acaataatcc gagacatggt gataagtgat
     2041 aagtgatgag tgataagaga gagaagacag gttttgtctt atcactcatc actcatcact
     2101 catcacgtcc tttcagcttg ccttctccag cttgatcggc cgctcgataa agtagaagct
     2161 gcactggccg cggacttcgt cgaggtcgcg gcgctcgatg ggatagagac ggcactgatc
     2221 ggggcggtct tcgtagatgc cgcaggtggt cgtaaagtcg tcgtgcttct tgaggaaggg
     2281 gcacttgaag agaatttcgc agcaggcgcc gcagcggttg cagtcgcccc ggcgcagctc
     2341 gagcgcctca tcgacctcct ccttgcggaa gcggatgttg acgaagcgtc ggagcttgcc
     2401 ctgcacctgg cgcgcgcgga acttgccctc ctcgacggcg cgcgtgcgct tggccgcgag
     2461 ccgctcctcg aaggtgcgcc gttgtgccgc gcgaatgcga acgctgtgtg atggtttcat
     2521 cgcgtctata gcccgccggg cgcgcccgct cgtcctgtct cacttcggac gcgccgccgg
     2581 cgcgcgggta taaccgctcc ggccctcaaa cacacgacgg cgcgagcgcg cgtccatata
     2641 aaaaacaccg cccgcgccgt cagtctcgcc cgcggcgcgt cagcccgcgc cgaccgcccc
     2701 gccgatgtgc ttctcgatca tgcgcgagat ggcctccttc gaggtcgcgc ccaccactcg
     2761 ctcctcctcc ttgccgttct tgaagagaat cagcgtcggg atgcccttga tgccgtagcg
     2821 ctgcgaggtc gccgggttgt cgtccacgtt cagtttgacg acgcgcgcgc cctgcgcgaa
     2881 cttctcggcc acggcctcga ccgtcggcgc gagcatccgg cacggcgcgc accacgcggc
     2941 ccagaagtct acgagcacgg gcttgtccga cttgagaacg tccgactcga agttggtgtc
     3001 gctaacgtcg ttaacatgtt cgctcatgta atatctcctt ctcgttccaa atctccgacg
     3061 gccatttcgg gacatcaaac gcgcgccgtc gtgtcacctc tgtccgcgat aaccggccca
     3121 ctactttaga tgcaacacgg gtgcaccgcc attctacaac gaggcgaccc gccgataaat
     3181 ctccagatac ctgcgcgccg cggccgacca tgaattgtcc tcgcgcatcc cgttcaactg
     3241 gacgtgacgc cacagcgccg gctgcccgta gcagtgaagc gcctcgtgga gtgcgcccat
     3301 catcgcgtcg gccgtgtagg ggccgaactt gaagccggcg ccggcgccgc gcgagcggtc
     3361 gaagtgctcc accgtgtcgt cgaggccgcc cgtggcgcgc accacgggga cggtgccgta
     3421 gcgcatgctg tacatctggt tcaacccgca cggctcgtac tgcgagggca tcaggaaaat
     3481 gtccgccccg gcctcgatct gatgcgcgag cggctcgttg aagccgcggt agacgccgac
     3541 gcggcgcggc gcgtgctcgt gcaaagcctg caaaaaatct tcgtactcgc gcgaacccga
     3601 acccagaaag accagaaaag cgcccgtctc caaaagtcgc ccggccgcct gcttgatgag
     3661 atcgaagccc ttctgcggcg tcaggcgcga gacagtggcg acgatgggcc gatgcaactc
     3721 ctcctcgggc agcccgaagc gccgcagcag gtcgcgcttg cactcgcgct tgcccgagag
     3781 gtctgccgcc gagtagtggg ccgcgaggtg cgggtccgtt ccggggttcc agacctcgta
     3841 atcgacgccg ttggtgatgc cgatgagccg gtcgcggcgc tggcgcagga gccattcgag
     3901 gccgtagccg tgctcgcgcg tctgtatctc ctgcgcgtag cggcggctga cggtcgagag
     3961 cgcgtcggcc gtcatcagtc cggccttcat ggccgaggcc acgttgttcg acagaaagac
     4021 gttcgtctct tcgccgggcc ggaagccgag ccgccacaac tccgtggggt cgaacatgcc
     4081 ctgaaaggcc agattgtgaa tcgacaaaag cgtgcgcgtc tccgagaggt aaccgcccca
     4141 cggccggccc gcgcgaatcc aggcggaggc gaagccgcac tgccagtcgt tcatgtggac
     4201 gatgtcgggg gcctcgccga gccggccgag gagcgtcagc gccgcgcggc taaagaaggc
     4261 gaaacgctcg tggtcgtctc gatggccgta aatcgtcgcg cgcatgaagt actcgggcgc
     4321 gtcgatgaga aacgtcgggg cgcccgccgc ctcgctgtac cagacccatg tgcggcgaat
     4381 ccggccgagc cattccacgt ccagattgtc aaagatcggc tgcgcgctca ggaggtcgcg
     4441 cttcgtctca ccgtagagcg gcgtgacgag tgttacgtcc gcctcgcccg tctcgatcag
     4501 ggctttcgga agcgcgcccg aaacgtcccc cagacctccc gttttcgagt acggaaccgc
     4561 ctcggacgat aaaaacgcga cgcgcaactt tttcagacac ttcgccccga gaggagacgc
     4621 cttcctgact catcgaattg gtaaagcttg gagggaatac tatgccaaaa gacgtgataa
     4681 gtgacaagtg atgagtgata agaaagagaa gacggttgtt gtctgatcac ttatcactca
     4741 tcacttgtca tcggctctta cgtctgcatc gaggcgagga actcggagtt tgtcttggtc
     4801 ttgccgaggc gttcgaggac gacctccatc tgctcgacgg gcgagagcgg gttgagcacc
     4861 ctccgcatca cccagatgcg attgaggtct tccttcgaga tcagcaactc ctccttgcgc
     4921 gtgccggagc gctggaggtc gatggccggg aagaggcgct tgtcggagag gcggcggtcg
     4981 aggtgtatct cggagttgcc cgtgcccttg aactcctcga aaatcacgtc gtccatgcgc
     5041 gagccggtgt cgatgagcgc ggtggcgatg atggtcaagg agccgccctc ctcgatgttg
     5101 cgcgccgcgc cgaagaaacg cttggggcgc tggagggcgt tggcgtcgat accgcccgaa
     5161 aggattttgc cggagggcgg gacgacggcg ttgtgtgcgc gggcgaggcg ggtgagtgag
     5221 tcgagcagga tgaccacgtc acgtttgtgt tcgacgaggc gcttggcctt ctcgatcacc
     5281 atgtcggcga cctgcacgtg gcgggtcggc ggctcgtcga aggtcgagga gatgacctcg
     5341 ccctgcaccg agcgctgcat gtccgtgacc tcctcggggc gctcgtcgat gagcagcacg
     5401 atgagcgaga cctcggggtg gttctgcgac accgagttgg cgatggcctg aagcatcatc
     5461 gtcttgcccg tgcggggggg cgcgacgatg agcgcgcgct ggcccttgcc gagcggggtg
     5521 acgaggtcga tgacgcgccc gacgatgtgg tcggcgttgg tctccatccg cagttgcgac
     5581 tccgggtaga gcggcgtcag gttgtcgaag aagaccttgt cgcgcgtctc gccgggcgct
     5641 tcaaagttga tggcctcgac cttgatgagc gcgaagtaac gctcgccctc cttgggcggg
     5701 cgaatctgcc ccgagacggt gtcgcctgtc cgcaggtcga acttgcgtat ctgcgagggg
     5761 ctgacgtaga tgtcgtcggg gccgggcaga tagttgtact cgggcgcgcg caggaagccg
     5821 aacccgtcgg gcagggtttc caaaacgccc tcggagaaga tgaggccgct cttctcggtt
     5881 tgcgcggcga ggaccttgaa gatcagctcc tgcttacgca tgcctgtggc gccgggcacg
     5941 tcgaggtctt tggcgatctg cgtcagcttg gagatggaca tctccttgag cgtcgccagg
     6001 ttgaaggtcg agccgtcgaa gacgaagccg atgccgtcat cgccatcgcc ctcaccgccc
     6061 tcgccttcgc ggtccgtccc gatatccacg tccggcggcg gaggaggcgg cggctcgatg
     6121 gcggcggtcg ccttgacggc cgcatcggcg tcggcgccgt tcgcgttgtc ggatgactta
     6181 atagtgcgtg ttctgcgtgt tgacatttcc gttcttcttt tgtgggcgcc gcctcgaccg
     6241 agcgggcagc gccgggattt cattcaagag tgcgtggaag cgcctgatcg atgggcgctc
     6301 ccgtttgtct tcgcgtcgat attggcgcgt gcctgttggc tggcgcgccg cggccgtttc
     6361 gttgaaatga ttgccgatgc cgtccggggg tgatgcctct ggggcgcgcc gttttttcgg
     6421 ggcgcgtcgg gtagggccgc gagccctgtg gctcggcgcg ggcggcctgc cgtgagaccc
     6481 cgcgcgcttc ggtggcgccc cactcaccgg ggcgccggtt aatcttcaaa tcctctcggg
     6541 tgtcgtcgct ggcatttttg atgcgcggca agcccgcccg tgttcgcttc gcgctctacg
     6601 aagtggggat ttttgaggcg gccagggcct cttcgatagc gcgccagacg gattcgcgtc
     6661 cgcgtcccgt cacagcggag taggcgacga tctcactcgc cccatcatcc cccaccccaa
     6721 gtatgcggtg agcgcgcgcg agggatttgc gcaactcgtt tgcggaaagc ttgtcagact
     6781 tcgtggccac cgtcacagac gcacgtccat gaaactcaag ccactccctg agctgtaagt
     6841 ccaattttgt gggatcgtga cgcgaatcta caatgtgaat tgatagcaca agcgacgtgc
     6901 gcttggcaag gtattcggtg acgaggtggc cccactcgcg gcgcacttcc tgcgggacgc
     6961 gagcgtatcc atagcccggc acatcgacga aataaaagcg ccggttgatg aggaaatagt
     7021 tgagcgactg ggtgcgcccc ggcgtcgagg aggtgcgcgc cagtcccttc acgccgagga
     7081 gcgaattcat caaactcgac ttccccacgt tcgagcgccc gaggaaggca acctccggca
     7141 gagagtcgcg cggccactgc gactcctcga aggcactctt gacgaaatcc gcgctcgcga
     7201 ctttcataaa aacaaactta aagcgtgaat taactcaact cacgaaacct cttcgcaccc
     7261 cggcggcgag ccatgaacga cgtgacgcgg gcgcgcgctt caaactagag ttggacggtg
     7321 ctgtttgttg aaggaagcaa aaggggaact tgggcgaggc tcagataaaa ataaaacgcc
     7381 gtgcggacga ctccggccag agtcgtccgc acgggcattg tggcgtgtca gtcggccatc
     7441 gtcggcgtac cctgcgtggg cgtgtcggtc gtccagacgg gcgggtgctc ggcgccgctg
     7501 ccttcgccgg cgctcgtggg gcacgcctct tcgagggcga gcgcgatgac ctcgtcaatc
     7561 gtctcgacga gctggacgcg caactcggag cgcacctcct cgggcacctc caacaagtcc
     7621 ttctcgttgt ccttcgggat gagcacgctc gtgatgccga agcggtgcgc ggccagcaac
     7681 ttctccttga tgccgccgac gggcagcacc ttgccgcgca gggtgatctc gccggtcatc
     7741 gtcacgtcgt tgcggacggg gacgcgcatc agcgccgagg ccatcgccgt ggcgatggtc
     7801 acgcccgccg acgggccgtc cttcgggatc gcgccctcgg ggatgtggat gtggaggtcg
     7861 cgcttgcgga tgaaatcgag gttgacgccc agacgctcgg cgcgcgcctt gatgcaggtg
     7921 agcgcggcct gcgccgactc ctgcatcacc tcgccgagct tgccggtgag tctgacgccg
     7981 ccgcggccgt ttgtcagcgt cgcctcgatc tggaggactt cgccgccgac ctccgtccac
     8041 gccagcccca cggccagacc gatctcgctg gtggcgtgcg ccgtgagttg ccggaacttg
     8101 atggggccga gcatttcgcg cacgcgctcg gccgtcaaca cctcgcggaa ctccgcgccg
     8161 tggctctcgc cgacgagctt gcgggccgtc ttccgcatgc acgagccgac ctcgcgttcg
     8221 aggttgcgca ccccggcctc gcgcgtgtag tggcggatga tttcgaggat gccctcgtcg
     8281 gtgaagtcta cctgctcggg cttgatgccg ctcccctcgg cctgcttgac gacgaggtgg
     8341 cgcttggcga tctgcaactt ctcgcgctcg gtgtagcccg acaggcggat aatttccaga
     8401 cggtcttgca gcgcgggcgg gatcgtgtgc agcacgttgg ccgtggcgat gaagaagacc
     8461 ttcgagaggt cgtactccac gtcgaggtag tgatcctgaa acgtgttgtt ctgttctggg
     8521 tcgaggactt ccaagagggc ggccgacggg tcgccgcgga agtctgcgcc gagcttgtcc
     8581 acttcgtcga ggaggatgag cgggttgacg gtgccggcct tcttcatcat ctggatgatc
     8641 tggccgggga gcgccccgat gtaagtgcgc cggtgcccgc gcacctcggc ttcgtcgcgc
     8701 acgccgccca gagagagacg cacgaacttg cgtcccgtgg cgcgggcgat ggacttgccc
     8761 agagaagtct tgccgacgcc cggcgggccg acgaagcaga ggatcgagcc tttgggattt
     8821 ttgacgagct gacgcaccgc gagatattcg aggatgcgct ccttgacctt ctcaagaccg
     8881 aagtggtctt cgtttaagat gtcttcggcg cgcgacaggt ctttgaggtc tttcgtcttc
     8941 tgcttccagg gcacgctcag gagccagtcg atgtaggtgc gcgagaccgt gccctcggcc
     9001 gacatcggcg gcatggcttc gaggcggtgg agttcctgga gggctttctc cttcgcctcc
     9061 tcggtcatgc cggcctcgtc gatgcgcttg cggaggtctt cgagttcggc cttctcgtcc
     9121 ttgcggccaa gctccttgtg gatggccttg atctgctcgt tgaggtagta ctctttctgc
     9181 gcgcgctcca tctgccgctt gacgcgggcc tgaatggtgc ggtcgagctg ccgcttatcc
     9241 acctcgactt cgagcagctc gacgagacgc tgaaggcggc ccgtcgtgga gaagatttcc
     9301 agaagaccct gcttgtcctc gacggagatt ttgatctggg aagccaaggc gtcggcgagg
     9361 tgcgaggaat cctgattgcg gagcgcggtc tggagcgcgt ccgtttgc
//
LOCUS       DASVES010000165         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1658286, whole genome shotgun sequence.
ACCESSION   DASVES010000165 DASVES010000000
VERSION     DASVES010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658286"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VF240_06690"
     CDS             complement(<1..324)
                     /locus_tag="VF240_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174950.1"
                     /translation="MNALIILLTLGLAAAVVYLTGSDGAAALLLCMALAVPCGALINR
                     AGGRFLLRVFVLGVLVRVAVGTLIFHAGMQEFFGGDALTYDFQGTEVYRYWTGQIQYR
                     EIRFDL"
     gene            complement(485..1849)
                     /locus_tag="VF240_06695"
     CDS             complement(485..1849)
                     /locus_tag="VF240_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174951.1"
                     /translation="MFIKQGRQRLIAGAVLIHLGLALAVFLIGRYAVLAALFDPHGTG
                     LRFASDGIAYMSEVRALVDMLTREGPSVWLAANYPLHVKIYSVSFAVLSPIFGFSTLS
                     AEPLNLFYYVAIVYLVFKLGEEAFGERAGIVAAWIVALWPSFLLHTTQLLKDPLYIAA
                     MLALLTVNLRWLTKDHPARGALLWGLAGGALCTLLWVVRSENGELTLAVALIGTVLVL
                     IRQFAHGRAARWNLAGAALMLLLAVGAPRVIYARRSVQAKPAVVVAAAPSSSVVAPSS
                     SVNAAKAQQGNSPADRVARLRVAFARSYPNAGSNVDADVSFDDAAGLVRHLPRALMVG
                     LLAPLPDMWFAPGAHTGRAGRMLSGAETLLMYVLQLFTLYGLWSQRRSLSVWLLLIVA
                     LVGAAALGLVVVNVGALYRLRYSFWMPLIVLGAGGAVQLYHALAERRREQSGRDLLPT
                     TQEA"
     gene            2158..3879
                     /gene="murJ"
                     /locus_tag="VF240_06700"
     CDS             2158..3879
                     /gene="murJ"
                     /locus_tag="VF240_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7174952.1"
                     /translation="MSTFREPDSPAAAAAQTAEGERRQVIRSAGVVSVAVLASRVLGL
                     VREQVFAAYFGAGFLNDAFQVGLRILGPLRDLFAEGALSVAFVKTFTDYCVRRGEEEA
                     WRLASMVLNALLVVLSVVVLAGIFFAPQIVALIAGDFPAEKAALAVTLTRIMFPFLLL
                     IALAAVAMGVLNTKGRFAVPASASLMFNVGSIAGGLLFAYWLSGGGWTSVRDPGAVPE
                     SAAQWAIIGMAIGTLIGGGLQFLVQLPSLLGVGFHYRPLLSFTDPGVRQVMRLMGPAV
                     VGTAAVQVNVLVNTYFAASIDGGPSWLGYAFRLMQFPIGLFGIAVGTAVYPTITRYAV
                     EDNQHGFRSMLSSSLGLVFLLSVPSACGLIVLARPIAALIYERGAFTSSDTRMVAAAL
                     AAYAVGLAGYAAIKVLLPAFYALGDARTPMLTSLLSIVVNVALGSMLMDWLSGYGVTA
                     ETPRGYAHAGPALATSCVVLLNFAALSLLMRRKLRRLEGRRILSSFARIALASTALSL
                     ASHLTYRALAAALGEASLGARLAVVAGSIAVGGAVFFASARLLSVRELSMAVGAITER
                     LRKGGKD"
     gene            complement(3905..>4342)
                     /locus_tag="VF240_06705"
     CDS             complement(3905..>4342)
                     /locus_tag="VF240_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174953.1"
                     /translation="GMIYLLFAPFPWQLVNLRQSLTLPEMLVWWGAFPFLVVGLWFTV
                     RYRMRQALPILVFTTMLTLAYSVFQGNVGTAYRQRSQLLVFYFIFASVGLVIARESRD
                     DKRLEARAANLRNQRATALAMARRKQAQEWESIADHLSEKIGF"
BASE COUNT          695 a         1508 c         1434 g          705 t
ORIGIN      
        1 caggtcgaac ctgatctccc tgtattggat ctgccccgtc cagtagcgat agacctccgt
       61 gccctgaaag tcgtaagtca gcgcgtcgcc gccgaagaac tcctgcatcc cggcgtggaa
      121 gatgagcgtc ccgacggcga ctcggacgag gacgccgagg acgaagacgc gcagcaggaa
      181 gcgcccgccc gcgcggttga tgagcgcccc gcagggaacg gcgagggcca tgcagaggag
      241 cagggcggcg gccccgtcac tccccgtgag gtagacgacg gcggccgcca gcccgagcgt
      301 cagcagtatg atcagcgcgt tcatggcttc ggttgcgcgg ggcactgcgg cgcgcccttt
      361 tgcgttatct cacaagaccc gcgcgggcct cacgccgcag gcacgccaga tgttacttca
      421 aacatttacg aaagctaagc ggcgcgggcc gtggctcccg gcgacggccc gcggggccga
      481 ggcgtcaggc ttcctgtgtg gtgggaagca ggtctctccc cgactgttcc cgacgcctct
      541 ccgccagcgc gtggtagagc tgcacggccc cgcccgcgcc gaggacgatg aggggcatcc
      601 agaaggagta gcgcaggcga tagagggcgc cgacgttgac gaccaccagc ccgagcgccg
      661 ccgcgccgac gagcgccact atgagcagca gccacacgga caggcttcgg cgctgactcc
      721 agaggccgta gagtgtgaag agctgcaaca cgtacatgag cagcgtctcc gccccgctca
      781 gcatccgccc ggcccggccc gtgtgggccc cgggcgcgaa ccacatgtcg ggcagcggcg
      841 ccagcagccc gaccatcagg gcgcgcggca ggtggcgcac gagaccggcc gcgtcgtcga
      901 aactcacgtc ggcatcgacg ttcgagccgg cgttcgggta tgagcgggcg aacgcgacgc
      961 gcagacgggc cacgcggtcg gcgggcgaat tgccctgctg agccttcgcg gcgttcacgg
     1021 aagacgacgg cgcgacaaca gaggatgacg gcgcggcggc gacgacgacg gcgggtttcg
     1081 cctgcacact cctgcgcgcg tagatcacgc gcggcgcgcc cacggccagc aacagcatca
     1141 gcgccgcccc ggccagattc caacgggcgg cccgcccgtg agcgaactgc cggattaaga
     1201 ccagcaccgt gccgatcaag gcgacggcca gcgtcagctc cccgttttcg cttctcacga
     1261 cccacagcag cgtgcagagc gccccgcccg ccagtcccca gaggagtgcg ccgcgcgccg
     1321 ggtggtcttt cgtcagccag cgcaggttga ctgtcagcag ggccagcatg gccgcgatgt
     1381 agagcgggtc tttgagcagt tgggtcgtgt gcagcagaaa cgacggccag agcgcgacga
     1441 tccaggcggc gactattcct gcgcgctcgc cgaacgcctc ctcgcccagc ttgaagacca
     1501 ggtacacgat ggcgacgtag tagaacaggt tcagcggctc ggcgctcagc gtgctgaagc
     1561 cgaagatggg gcttaagacg gcgaaggaga cggagtagat tttgacgtgg agcgggtagt
     1621 tggcggcgag ccagacggag gggccttcgc gcgtgagcat gtccacgagc gccctgacct
     1681 ccgacatgta ggcgatgccg tcggaggcga agcgcagacc cgtcccgtgg gggtcgaaca
     1741 aggcggcgag gactgcgtag cggccaatca ggaaaacggc cagtgccaac ccgaggtgta
     1801 tcaacaccgc ccccgcgatc aaacgctggc ggccctgttt aatgaacaaa atattatctc
     1861 cggcgctgct ttatgagttt ttgtaccata agtggtgtgg cgacaccaga agggtgtggg
     1921 aagggtcgcc gtgccggcgc cgcgcgcgcg ggcgcttttc acgtggggcg gctgatgtta
     1981 gttttgtgcg cgctctggcg aaacaactat agctctttgc ggatgggcgc gtccgtccgc
     2041 cacaggactg aagagaacac ggactaaacg gatgaagacg gatgaagacg gattgaatcc
     2101 gtgctcatcc gccttatccg tttaatccgt gttgcctcct cgcccgcccc agaccttatg
     2161 agcaccttcc gcgagccaga ctcgccggcc gccgccgccg cgcagaccgc cgagggcgag
     2221 cggcggcagg tgatccgctc ggcgggcgtc gtctccgtcg ccgtgctggc ctcgcgcgtg
     2281 ctggggctgg tgcgcgagca ggtgttcgcc gcctacttcg gcgccggctt cctcaacgac
     2341 gccttccagg tgggcctgcg catactcggc cccctgcgcg acctcttcgc cgagggcgcg
     2401 ctctccgtcg ccttcgtcaa gaccttcacc gactactgcg tccgccgcgg cgaagaggaa
     2461 gcgtggcgtc tcgcgagcat ggtcttaaac gcgctgctgg tcgtcttaag cgtcgtcgtc
     2521 ctcgccggaa tcttcttcgc cccgcagatc gtcgccctca tcgccggcga cttccccgcg
     2581 gagaaggccg cgctcgctgt cacgctcacc cgcatcatgt tccccttcct gttgctcatc
     2641 gcactcgccg ccgtggcgat gggcgtgctc aacacgaagg gccgcttcgc cgtcccggca
     2701 tcggcctcgt tgatgttcaa cgtcggctcc atcgccggcg gactcctctt cgcctactgg
     2761 ctgagcggcg gcggctggac gagcgtgcgc gaccccggag ccgtgcccga atcggccgcg
     2821 cagtgggcca tcattggcat ggccatcggc acgctcatcg gcggcggcct ccagttcctc
     2881 gtccagttgc cctcgctgct cggagtcggg tttcactacc gccccttgct gagcttcacc
     2941 gacccgggcg tgcgacaggt gatgcgcctg atggggccgg ccgtcgtcgg cacggccgcc
     3001 gtgcaggtca acgtgctcgt caacacctac ttcgcggcga gcatcgatgg cggcccgtcg
     3061 tggctcggct atgcgttccg gctgatgcag tttcccatcg gactcttcgg catcgcggtg
     3121 ggcacggccg tctacccgac catcacgcgc tacgccgtcg aggacaacca gcacggtttc
     3181 cgctcgatgc tctcctcctc gctcggactc gtcttcctcc tgagcgtccc ctcggcctgc
     3241 ggcctcatcg tcctcgcccg gcccatcgcc gcgctcatct acgagcgcgg cgctttcacg
     3301 tcgagcgaca cgcgaatggt cgcggccgcg ctcgccgcct acgccgtggg gctggccggc
     3361 tacgccgcca tcaaggttct gctccccgcc ttctacgcgc tcggcgacgc gcgcacgccg
     3421 atgctcacga gcctgctctc gattgtcgtc aacgtcgcgc tgggcagtat gctgatggac
     3481 tggctctccg gctacggcgt gacggcggag acgccgcgcg gctacgccca cgccggcccg
     3541 gcgctggcga cctcctgcgt cgtcctcctg aacttcgcgg cgctctctct gctcatgcgg
     3601 cgcaagctca ggcgtctgga ggggcggcgc atcctctcgt cgtttgcgcg catcgcgctg
     3661 gcctcgacgg cgctctctct ggccagccac ctcacctacc gcgcgctcgc ggcggcgctc
     3721 ggcgaagcca gcctcggcgc gcggctcgcg gtcgtggccg gctcaatcgc cgtcggcggc
     3781 gcggtcttct tcgcgtcggc gcggctgctc tcggtgcggg agttgtcgat ggccgtgggg
     3841 gcgattaccg aacggctcag gaagggcggg aaggactagc gccggggcgg cgcgcgcact
     3901 cttttcagaa acctatcttc tccgacaaat ggtcggcaat cgactcccac tcctgcgcct
     3961 gcttgcgccg ggccatggcc agcgccgtcg cgcgctgatt ccgcaagttg gccgctcggg
     4021 cctccagccg cttgtcgtcc ctgctctccc tggcgatgac gaggccgacg gaggcgaaga
     4081 tgaagtagaa gacgagcagt tgcgagcgct gccgataggc cgtcccgacg ttgccctgaa
     4141 agaccgaata cgccagcgtc agcatcgtcg tgaacaccaa tatcggcaac gcctgccgca
     4201 tccggtagcg cacggtgaac cacagcccga cgacgaggaa ggggaaagcc ccccaccaca
     4261 ccagcatctc gggcagcgtc aggctctgcc gcaggttgac caactgccac gggaacggcg
     4321 cgaacaacag gtagatcatc cc
//
LOCUS       DASVES010000166         4835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_536994, whole genome shotgun sequence.
ACCESSION   DASVES010000166 DASVES010000000
VERSION     DASVES010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536994"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2075)
                     /locus_tag="VF240_06710"
     CDS             complement(<1..2075)
                     /locus_tag="VF240_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7174954.1"
                     /translation="MDTLVEQLRAATAVGRVLAEPHELLVYECDALTHYKARPRAVVF
                     PDSTEEVAGVVRVLAREGVPFAPRGAGTGLSGGALALRGGVVIELARMRKILRLDAAN
                     RLAVVQTGVVNAHLTRAAAPHGLHYAPDPSSQATCTIGGNLAENAGGIHCLKYGTTVD
                     HVAAARVVLSDGEVIDLGGAASADAPGYDLLGVFVGSEGTFGIATEVSVRLTPLPQAV
                     RTVLADFRVIDDASRAVSAIIAEGLIPAALEMVDGATIRAVEASVFAAGLPTDAEAAL
                     LVELDGLEAGLDAETARVENICRRHGARTCRRARDEGERQKLWAARKGAFGAMGRITP
                     DLMLQDAVVPRSRLPEVLADTYRIGAKYRLRVANVFHAGDGNLHPYICFDARNADEVA
                     RVKEAGREIMETCVRAGGTITGEHGTGLDKSDYLPLVFSSDTLRAMLEVRSAFDPLGL
                     CNPGKVIPAPRGCGEARAVASRGAVRSKEGGGHDGDEARTAGEARDVGKGLSQVGASA
                     NANARAATHIEAHAATRADADASSTSTHVSSTHVTVATRARGARGRFEPAKALDALAE
                     VVGPQNVSLRGESVVAAPCTTAEVREVLRLTGGTGWPVVPAGGETWTDAGNTLACAGR
                     VVVNTTRLTGVIEHEPADLVATARAGTTLVHFNGALALARQWLPLDPPDDGRATLGGV
                     AATGTGGAQS"
     gene            2322..2717
                     /locus_tag="VF240_06715"
     CDS             2322..2717
                     /locus_tag="VF240_06715"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7174955.1"
                     /translation="MWEFNDKRVAPRFTTRLRCRLLFPATESAEGGRRHAPAAPLVGY
                     TRDVSSSGLAVIVSNISSAGAHLTDLDATVLVELDLPSGQIEVQTAPARIERLPGGDA
                     DSGYLVGLRITKIHESLRARFEQFIYTLG"
     gene            2747..4429
                     /locus_tag="VF240_06720"
     CDS             2747..4429
                     /locus_tag="VF240_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7174956.1"
                     /translation="MRKSTSVRLAACAALASIVFSAPARCQEMKASLIITNANVRTMD
                     AKRPEAQAVAVFGNRVAAVGSTAEVERLKGPATRVIDAGGALVLPGFNDSHTHFLGGG
                     FQLSSVDLRDAATPQEFAERIRAFAAKTPKGRWVTGGDWDHERWPGAPLPTRELIDSF
                     TPDTPVFVSRLDGHMALANTHALRLAGVTKETKDPPGGLIVRDPKTGEPTGILKDNAM
                     SFVFSKVPDASFEEKLTAARAATEHAARLGVTSVQDMSAGSDVSVYQTLLDRGELKTR
                     VYAVAPLGAWKRLADVGVRANFGGDMLRIGGLKAFSDGSLGSTTALFFEPYNDAPETR
                     GLGSGLAEGEMLGRVQSADAAGLQVMIHAIGDRANDQILSIYEQVAREHGERDRRFRI
                     EHAQHLRREEIREFARQKVVASMQPYHAIDDGRWAEKRIGPERIKGTYAFRALLDAGA
                     VLAFGSDWTVAPLDPILGVYAAVTRRTLDGKNPKGWVPEQKITVEEAVRAYTFGSAYA
                     EFAEKVKGAIMPGMLADIVILDRDIFRIDANDIEKARVTMTIVDGRVVYEKK"
BASE COUNT          815 a         1673 c         1595 g          752 t
ORIGIN      
        1 gactgtgcgc cgcccgtgcc ggtcgcggcg acgccgccga gcgtcgcgcg cccgtcgtcg
       61 ggcgggtcga gtgggagcca ttgtcgcgcg agcgcgagtg cgccgttaaa gtggacgagc
      121 gtcgtgccgg cgcgggcagt ggcgacgagg tctgccggct cgtgctcgat gacgccggtg
      181 aggcgcgttg tgttgacaac gacgcggccg gcgcacgcga gcgtgttacc ggcgtcagtc
      241 caagtctcgc cgccagcggg cacgaccggc cagcccgtgc ccccggtcag cctcaaaact
      301 tcgcgcactt ccgccgtcgt acacggcgcg gccacgaccg actcacccct gagcgagacg
      361 ttctgcggcc caacgacttc ggccagcgcg tcgagcgcct tcgccggctc gaaccggcca
      421 cgcgcaccgc gcgcgcgcgt ggcaacagtg acgtgcgttg aagagacgtg agttgaagtt
      481 gaagaagcgt cggcgtccgc gcgcgtcgcg gcgtgggcct ctatgtgcgt cgcggcgcgg
      541 gcgttcgcgt tcgccgacgc gcccacctgc gacagacctt taccaacatc tcgcgcttca
      601 ccggccgtcc tcgcttcatc cccgtcgtgt cctcctcctt ccttgcttcg caccgcgccg
      661 cgagacgcga cggcgcgggc ttcgccgcag ccgcgcgggg cggggatgac tttgccgggg
      721 ttgcacaagc ccaacgggtc gaaggcggat cgcacttcga gcatcgcgcg gagcgtatct
      781 gatgagaaga cgagcggcag gtaatcgctc ttgtcgaggc ccgtgccgtg ctcgcccgtg
      841 atggtgccgc cggcgcgcac gcaggtctcc ataatctcgc ggccggcttc cttgacgcgc
      901 gcgacctcgt cggcgttgcg cgcgtcgaag cagatgtacg ggtggaggtt gccgtcgccg
      961 gcgtggaaga cgttggcgac gcgcaagcga tatttagctc cgatgcgata agtgtcggca
     1021 agcacttcgg gcagacgcga gcgcgggacg acggcgtcct gcaacatgag gtcgggcgtg
     1081 atgcggccca tcgcgccgaa ggcgccctta cgcgccgccc acagtttctg tcgctctccc
     1141 tcgtcgcgcg cgcgccgaca agtgcgcgcg ccgtgacgcc ggcagatgtt ctccacgcga
     1201 gccgtctcag cgtcgaggcc cgcctccagt ccgtcgagtt cgacgagcag cgcggcctcc
     1261 gcgtccgttg gcaaccccgc cgcgaagacg ctggcttcga cggcgcggat ggtcgcgccg
     1321 tcaaccatct ccagcgccgc cgggattagc ccctcggcga tgatggccga gacggcgcgg
     1381 cttgcatcgt cgatgacgcg gaagtcggcg agcaccgtgc ggacggcctg cggcagcggc
     1441 gtcagacgaa cactcacttc ggtcgcgatt ccgaacgtcc cttcggagcc gacgaagaca
     1501 ccgagcagat cgtagccggg cgcgtccgcc gaagcggcgc cgccgaggtc gatgacctca
     1561 ccgtccgaaa ggacgacgcg cgcggccgcg acgtgatcga ccgtcgtgcc gtacttgagg
     1621 caatggatgc cgccggcgtt ctcggcgagg ttgccgccga tggtgcatgt ggcctgactc
     1681 gaagggtcag gcgcgtagtg caggccgtgg ggtgcggcgg cgcgcgtcag atgagcgttg
     1741 accacgccgg tctggacgac ggccagacgg ttcgccgcgt cgaggcgaag tatctttcgc
     1801 atccgcgcaa gctcgatgac gactccgccg cgcagcgcga gcgcgccgcc cgagaggccc
     1861 gttccggctc cgcgcggggc gaagggcacg ccttcgcgcg cgagcacgcg cacgacgccc
     1921 gcgacctcct cggtcgagtc ggggaagacg acggcgcgcg gtcgcgcctt gtaatgcgtc
     1981 agggcgtcgc actcgtagac gagaagttcg tgcggctccg cgaggacgcg cccgacggcg
     2041 gttgcggcgc gcagttgttc gaccagtgtg tccacgagat tcggtttgcg ggagaccttt
     2101 tttctaccgg aaggacaggg ctaatgatac actacgcgcg ctgcctcgcg tagcgcatcc
     2161 tcatacgcgg ttgacgtgtt cacacggctc ttttcccatc aaaacaggcg cgccgcgcgg
     2221 ccggggccgg agatggggcg gagtcaccga agtaagtgcg ggccgcgcaa ccgtcgagcg
     2281 gtagacctca ccgccacggc cgcttttgca cgggagcaca gatgtgggaa tttaacgaca
     2341 agcgcgttgc gccgcgattc acgacgcgcc tgcggtgccg acttctgttc cccgccacgg
     2401 agtcggccga gggcggcagg cgtcacgccc ccgcggcgcc gctcgtcggc tacacgcgcg
     2461 atgtcagctc ctcggggctg gccgtcatcg tctcgaacat cagcagcgcc ggcgcccatc
     2521 tcaccgactt ggacgccacc gtgctcgtcg aactcgacct cccctcgggg cagatcgagg
     2581 tgcagaccgc tcccgcccgt atcgagcggc tgcccggcgg cgacgccgac agcggttatc
     2641 tcgtcggcct gcggatcaca aagattcacg agagcctgcg ggcgcgcttc gagcagttca
     2701 tctacacact gggttagggc cgggacgaag gggaatcatt tctgacatga gaaagagcac
     2761 gagcgtgcgt ctcgcggcct gcgccgcgct ggcgtcgatt gttttcagcg cccccgcacg
     2821 ctgccaggag atgaaggcca gcctgatcat cacgaacgcc aacgtccgca cgatggacgc
     2881 gaagcgcccg gaggcgcagg ccgtcgccgt cttcggcaac cgcgtcgcgg cggtcggctc
     2941 gacggccgag gtcgaacgcc tgaaggggcc ggcgacacgc gtcatagacg ccggcggcgc
     3001 gctcgtcctc cccggcttca acgactcgca cacacacttc ctcggcggcg gcttccagct
     3061 ctccagcgtt gacctgcgcg atgccgccac cccgcaagag ttcgccgagc gcatccgcgc
     3121 cttcgccgcg aagacgccca agggacgctg ggtcacgggc ggcgactggg atcacgaacg
     3181 ctggcccggc gcaccgctcc cgacaagaga actcatcgac tcgttcacgc ctgacacgcc
     3241 cgtcttcgtc agccgactcg acggccacat ggccctcgcc aacacacacg cgctccgcct
     3301 cgcgggcgtg accaaagaga ctaaagaccc gcccggcggt ctcatcgtcc gcgaccccaa
     3361 gaccggcgag ccgaccggca tcctcaaaga caacgcgatg agctttgtct tctcgaaagt
     3421 ccccgacgcg tccttcgagg agaaactgac ggccgcgcgc gccgccaccg agcacgccgc
     3481 gcgcctcggc gtcaccagcg tgcaggacat gtcggccggc tcggacgttt ccgtctatca
     3541 gacgttgctc gaccgcggcg agttgaagac gcgcgtctac gccgttgccc cgctcggcgc
     3601 gtggaagcgg ctcgccgacg tgggcgtgcg cgcaaacttc ggcggcgaca tgctccgcat
     3661 cggcggcctc aaagccttct ccgacggcag cctcggctcg accaccgcgc tcttcttcga
     3721 gccttacaac gacgcgcccg agacgcgcgg tctggggagc ggcctcgccg aaggcgagat
     3781 gctggggcgc gtccagtcgg ccgacgcggc cggacttcag gtgatgatcc acgccatcgg
     3841 cgaccgcgcc aacgaccaga ttctctcgat ctacgaacag gtcgcgcgcg aacacggcga
     3901 gcgcgaccgc cgcttccgca tcgagcacgc acagcacctc cggcgcgagg agattcgtga
     3961 gttcgcccgc cagaaggtcg tcgcctcgat gcagccctac cacgccatcg acgacggccg
     4021 ctgggccgag aagcgcatcg gccccgagcg catcaagggc acctacgcct tccgcgcgct
     4081 cctcgacgcc ggcgccgtgc tcgccttcgg ctccgactgg acggttgccc cactcgaccc
     4141 catcttaggc gtctacgccg ccgtcacccg ccgcacgctc gacggcaaga acccgaaagg
     4201 gtgggtgccc gagcagaaga tcaccgtcga agaggccgtg cgcgcctaca ccttcggctc
     4261 cgcctacgcc gagttcgccg aaaaagttaa aggcgcaatc atgccgggga tgctcgccga
     4321 catcgtcatc ctcgaccgcg atattttcag gatcgacgcg aacgacatcg agaaggcgcg
     4381 cgtcacgatg acgattgtcg atggccgcgt cgtctacgag aagaagtgag cggtgagcgg
     4441 tgagcagtga gcagtaaaag aaagaaactg cttagagcat gtttagaaat tgtgttcagc
     4501 cgcgaagcgg caataagaag gtagccagac gtgaaacgtc tggtatcagc cgaataggtt
     4561 atcgcgctct gaaggagcgc gaggtgtttc ttcccgcgtc ttcaacgcgg gggaattatt
     4621 gggccgctgt cccagacgtt tcacgtctgg ctaccttctt tatgcgcctc cggcgcaaaa
     4681 cccggaatgt tcaaacatcc tcctactgct cactgctcac tcttcttaac ttgaaaactg
     4741 cataaacgac gaggccaacg gcaacggcga tgacggcgtc gcgcgcctcg cgtcccgtgc
     4801 tctgcgagag cagccagacg cagagcagta gcgcg
//
LOCUS       DASVES010000167         6783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1174642, whole genome shotgun sequence.
ACCESSION   DASVES010000167 DASVES010000000
VERSION     DASVES010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174642"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            549..983
                     /locus_tag="VF240_06725"
     CDS             549..983
                     /locus_tag="VF240_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7174957.1"
                     /translation="MSAAEFHPPHAVTPPHAAPARGPATVLLVEDDRALRRYLQVTLE
                     RAGFRVVVASDGLEAMKAALSAPVDAVVTDAIMPHLDGHALCRFLRRHPKLKSLPVVL
                     LSGSEQSAPSASPAERADAYLIKPVRPKELAGCLTALLERDA"
     gene            complement(1101..2255)
                     /gene="ald"
                     /locus_tag="VF240_06730"
     CDS             complement(1101..2255)
                     /gene="ald"
                     /locus_tag="VF240_06730"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX7174958.1"
                     /translation="MIVGLPKEIKDNEYRVGLTPAGVRALTDAGHKVVVERGAGEGSG
                     FESGLYEKAGARLIDAADDVWAEAEMVVKVKEPIAPEYPRMKEGLLLFTYLHLAPDPK
                     QTEALLQNKVTGVAYETITDRRGTLPLLTPMSEVAGRMAVQVGAQYLEKMNGGRGVLL
                     GGVPGVPAARVVIIGGGVVGTNAAKMAVGLGAQVTIVDRNLDRLRELDDIFLSKISTL
                     ASSAYAIHGAISEADLIIGAVLVPGAAAPRLITREMLKDVPNGAVIVDVAVDQGGCIE
                     TTRPTTHSNPTYYEEGVLHYCVANMPGAVPRTSTFALTNATLPYVLRLANRGLLEAVR
                     TDPGLKAGVNTYAGKLTYEAVAADQGHEYTSLDEMLGLPGETSTTAGSAG"
     gene            complement(2424..2897)
                     /locus_tag="VF240_06735"
     CDS             complement(2424..2897)
                     /locus_tag="VF240_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7174959.1"
                     /translation="MKVCVREVTSENREEALALSVWPEQREFVPTVARSLAKCHLKPD
                     GKTYEPFAVYSADELVGFYTLSYNPADATKCYLGGFLIDRAHQRRGLGSAALAQYLDE
                     VVRRFPSCEEVYLTVHSDNHAAEALYRGAGFEHLGFQVDGENAMRLRLTRARAGV"
     gene            complement(3078..4646)
                     /locus_tag="VF240_06740"
     CDS             complement(3078..4646)
                     /locus_tag="VF240_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7174960.1"
                     /translation="MSQAKKALSKRSARSASGTQSRPVAKSYKNYIGGEWVASQSGEW
                     FENTNPADTREVVGRFPVSTREDIYAAVAAAREAAERWRRLPAPRRAEILFRAGEILL
                     RDKDAYSRDMTREMGKVLKETGGDVQEAIDCTYYTAGEGRRLHGFTTPAEMPDKFAMC
                     VRQPVGLCGLITPWNFPMAIPSWKLMPALICGNTVVIKPAEDTPLSTYNLIRACEEAG
                     VPRGVINMVAGYGETVGAALTEHPDVRLISFTGSTEVGRIVASACAERNAICSLEMGG
                     KNVIMVMEDADLDLAVEGAVWGAFGTSGQRCTASSRLVVHKKVYKKFTQKLVERASAL
                     RVGNGLDAKTEMGPVINSDALHRILGYVETGQREDGARLACGGNRLDRGEHANGYFIE
                     PTVFTDVAPQMRIAQEEIFGPVTCVIPTNSLEEAVEIGNNVRYGLSAAIYTQDVNRAF
                     HAMNDMYTGIFYVNSSTIGAEVHLPFGGTKGTGNGHREAGTQVLDIFTEWKSVYVDYS
                     GKLQRAQIDEVQFE"
     gene            complement(4729..6318)
                     /locus_tag="VF240_06745"
     CDS             complement(4729..6318)
                     /locus_tag="VF240_06745"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX7174961.1"
                     /translation="MTEASKGQTNRAEQAMLRRLRVWIAALAALCLLVGLGLGAMLAS
                     KQAVAQNEAPIAQIARAPEALSASFAEIARRVEPAVVNIDTVSAPQIAAKDDDEEEDE
                     AEKEAPGGNSNPLLDMLRRRMRRPSRGVGSGFVIDPKGLILTNYHVVQGASKINVKLQ
                     SGATLRGEVAGFDEETDLAIVRVRPPQDLPAVQFGNSDDVQVGDWVLAIGSPFGLEQT
                     VTAGIISTRERQTDPSAAFQRFLQTDAAINRGNSGGPLVNMRGEVIGVNSQIATSTGD
                     YNGIGFALPANEAAFVYKQIVGGGKVRRGYLGIYLQPVRAEFAQVYGLAEAKGAIVRD
                     VADQNGPAAKAGILTNDIIVEFDGRPVTDAQDLINKVAATEVGRAVQVAYLRELNNKL
                     ERRTAAVTVGERPPSRTLQGDRKEEPDAKKKKSSAAPTDRPTLGLKVSELTDQLAEEK
                     NLRGLRGVIITDVEDGSLADDSGLDEGVVILRVNRVAVNSLADFQGFVNSLKPGDAVV
                     LHVANYQNDRILRRIVQFTFQ"
     gene            complement(6582..>6783)
                     /locus_tag="VF240_06750"
     CDS             complement(6582..>6783)
                     /locus_tag="VF240_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7174962.1"
                     /translation="GRMSATHAAIIFSLEPVFATIFAVWMRGAGEWTGGRANLGAALI
                     LAGIIVSELRWGERRAAGRTET"
BASE COUNT         1085 a         2229 c         2228 g         1241 t
ORIGIN      
        1 atggcgggtg tgacggcgaa ctcgtacatc ttgaaacttt agtcccggac agatggcgac
       61 tacaaggtcg gggccggcgg cccccgaaga aactacaagt atcggctaag acccacccgt
      121 ctatcaagcg cgcgggcacg ctcggccgtt tgtccgcccg tcttcggcag cggcttgcct
      181 gctcaactgc ggcgacttgc ccgctcaact gaggactcgt tttgatgctt tcacgttgtc
      241 ctttttgacc ccggcggcgc gccattctga ccggggccgg gccttcaaat tgaattgttg
      301 acaggcggcg cggtgaatcg ttaaggtgaa gttaccgggc cgcatggctc gcttctcccg
      361 aaagtctttg gcaatcttct aaaatcgagt cttttctcgg aaacagacgc gccctcacgc
      421 ttttctcgcg cggccacgtt tcgccccaaa gctgacccag ccgcgaggca cggggattgc
      481 catttttcgg tgcgcggagt ttcctcgcgc cccgccggat tgtcttcaga agctcacgcc
      541 ggagaccaat gagcgccgcc gaattccatc ccccccacgc tgtcaccccg ccacacgccg
      601 cgcccgcgcg cggccccgcc acagtcctgc tcgtcgaaga cgaccgcgcc ctccggcgct
      661 acctgcaagt cacactggag cgcgcgggct tccgcgtcgt cgtcgcctcg gacggcctcg
      721 aagcgatgaa ggccgcgctc tccgcgcccg tggacgcggt cgtcaccgac gccatcatgc
      781 cgcacctcga cggccacgcg ctctgccgct tcctgcgacg ccacccgaaa ctcaagagcc
      841 tacccgtcgt gctcctgagc ggcagcgaac agtccgcgcc cagcgcctcg ccggccgagc
      901 gcgccgacgc gtacctgatc aagcccgtgc gcccaaagga gctggccggc tgtctcacgg
      961 ccctgctcga acgcgacgca taaccatcca tcacgtccgc acgccctcgc gcgcttcaac
     1021 aggaaagggc atccttccgc gcggaaggat gcccttttcg gattctcgcc gttgcgacgg
     1081 tctgcgccgt cgctctcgtc ttaacctgcg ctgccggccg tcgtgctcgt ctcgccgggc
     1141 aggccgagca tctcgtcgag ggacgtgtat tcatggccct ggtcggcggc cacggcctca
     1201 taagtcagct tgccggcgta ggtgttgacg cccgccttga ggccggggtc ggtgcggacg
     1261 gcttcgagca ggccgcggtt ggcgaggcgc agcacgtagg gcagcgtcgc gttggtgagc
     1321 gcgaaggtgg aggtgcgcgg cacggcgccg ggcatgttgg cgacgcagta gtggagcacg
     1381 ccctcttcgt agtaggtcgg gttcgagtgc gtggtggggc gcgtcgtctc gatgcagccg
     1441 ccctgatcga ccgccacgtc cacgatgacc gcgccgttgg gcacgtcctt cagcatctcg
     1501 cgcgtgatga gacgcggggc cgccgcgccg ggcacaagca ccgcgccgat gatcaggtcg
     1561 gcctccgaga tggcgccgtg gatggcgtag gccgacgacg ccagagtgga aatcttcgac
     1621 agaaagatgt cgtcaagctc gcgcaggcgg tcgaggttgc ggtcaacaat cgtgacctgc
     1681 gcgcccaaac ccacggccat cttcgccgcg ttcgtcccca ccacaccgcc gccgatgatc
     1741 acgacgcgcg ccgccggcac gcccggcacg ccgcccaaaa gcacgccgcg cccgccgttc
     1801 atcttctcca gatactgcgc gccgacctgc acggccatcc ggcccgccac ctccgacatc
     1861 ggcgtcaaaa gcgggagcgt gccgcggcgg tcggtgatgg tctcgtaggc gacgcccgtc
     1921 accttattct ggagaagcgc ctcggtctgt ttggggtcgg gcgcgaggtg gaggtaggtg
     1981 aagagcagca gtccctcctt catgcgcggg tactcgggcg caatcggctc cttcaccttg
     2041 acgaccatct cggcctcggc ccacacgtcg tcggccgcgt cgatgagacg agcgccggcc
     2101 ttctcgtaga gtccgctctc gaagccggag ccttcgcccg cgccgcgctc gacgaccact
     2161 ttgtgtccgg cgtcggtgag ggcgcgcacg ccggccggtg tcagaccgac gcggtactcg
     2221 ttgtccttaa tctctttcgg gagtccgacg atcactgttc gtgttctcct gttctatatg
     2281 atccgggcct cggcgcgccg gggcagcagg cgcacgcact tcgtgtccgg gtttcagtcg
     2341 ctcacttgtg gaaactcatt ttgaccctgc gcgttgcgac acgcctgtga tacttcaaac
     2401 tcgtgcgaga gtcaaacaaa tacttaaacg ccggcgcgcg ctctggtcag tcgcaggcgc
     2461 atggcattct cgccgtccac ctgaaagccc agatgctcga atcccgcgcc gcggtacagc
     2521 gcctcggccg cgtgattgtc tgaatggacg gtcaggtaga cctcctcgca cgaggggaat
     2581 cggcgcacga cctcgtcgag gtactgggcg agcgcggcgc tgccgagtcc tcgccgctgg
     2641 tgcgcgcggt cgatgaggaa gccgccgagg tagcacttcg tcgcgtcggc cgggttgtaa
     2701 gagagcgtgt agaagccgac caactcgtcg gccgagtaga cggcgaacgg ctcgtaagtc
     2761 ttcccgtcgg gcttgaggtg acacttggcg agcgagcggg cgaccgtggg cacgaactcg
     2821 cgctgctcgg gccagacgga gagggccagc gcttcctccc gattctcgct cgtcacttcg
     2881 cggacgcaga ctttcatggg gagccgttag cgattagctg ttagcggtta gctaagagct
     2941 gtccgtagtc agtggtcagt tgttcgcagc gcccggcctt gagcctcact taatgggcgg
     3001 cgcgtctccc gaaacaaaca atcgacaact gaccacggac aaaaaagcta agagctaaca
     3061 gctaatcgct aatagctcta ttcaaattgg acttcgtcga tctgcgcgcg ctggagcttg
     3121 ccgctgtagt ccacgtagac gctcttccat tcggtgaaga tgtcgaggac ttgcgtgccg
     3181 gcctcgcggt ggccgttgcc tgtccccttg gtgccgccga aggggaggtg aacctcggcg
     3241 ccgatggtcg atgagttaac gtagaagatg cccgtgtaca tgtcgttcat tgcgtggaag
     3301 gcgcggttca cgtcctgcgt gtagatggcc gccgagaggc cgtagcgcac gttgttgccg
     3361 atctcgacgg cctcttccag cgagttcgtc gggatgacgc aggtgacggg gccgaagatt
     3421 tcctcctgcg cgatacgcat ctgcggggcc acgtcggtga agacggtcgg ctcgataaag
     3481 tagccgttgg cgtgctcacc ccggtcgagt cggttgccgc cgcaagcgag gcgcgcgccg
     3541 tcctctctct gccctgtttc gacgtagccg agaattcggt gcagggcgtc ggagttgatg
     3601 accggcccca tctcggtctt cgcgtcgagg ccgttgccga cgcgcaaggc gctggcgcgc
     3661 tcgacgagtt tctgcgtgaa cttcttgtaa accttcttgt ggacgacgag acgcgacgaa
     3721 gccgtgcagc gttggcccga ggtgccgaac gctccccaga ccgcgccctc caccgcgagg
     3781 tcgaggtcgg cgtcctccat taccatgatg acgttcttgc cgcccatctc cagagagcag
     3841 atggcgttgc gttcagcgca ggccgaggcg acgatgcggc cgacctccgt cgagcctgta
     3901 aacgagatga gacgcacgtc cgggtgctcg gtcagggccg cgccgacggt ctcgccgtag
     3961 ccggcgacca tgttgatgac gccccggggg acgcccgcct cctcgcacgc ccggatgaga
     4021 ttgtaggtcg agagcggcgt gtcctcggcc ggcttgatga cgaccgtgtt gccgcagatg
     4081 agcgccggca tcagcttcca cgacggaatc gccatcggga agttccacgg cgtaatcaac
     4141 ccgcacaggc cgacgggctg gcgcacgcac atggcgaact tgtcgggcat ctcggccggg
     4201 gtggtgaagc cgtgcagacg ccggccctcg ccggccgtgt agtaggtgca gtcgatggcc
     4261 tcctgcacgt cgccgccggt ctccttcaag accttgccca tctcgcgcgt catgtcgcgc
     4321 gagtaagcat ccttgtcgcg cagcaggatt tcgccggcgc ggaagagtat ctcggcgcgg
     4381 cgcggcgcgg gcaggcgacg ccagcgctcc gccgcctcgc gcgcggccgc caccgcggcg
     4441 tagatgtcct cgcgcgtcga gacggggaag cgcccgacga cctcacgcgt gtcggccggg
     4501 ttcgtgttct cgaaccactc gccggactgt gaggcgaccc actcgccgcc gatgtaattt
     4561 ttgtaagact tcgcgacggg gcgcgactgt gtgcccgacg ccgagcgcgc gctgcgtttg
     4621 ctgagtgctt tttttgcttg agacatagag gagtgatgag tgatgagtga tgagtgataa
     4681 gagtaagaaa gccgtctttc ttatcactca tcactcatca ctcatcactt attgaaatgt
     4741 gaactgcacg atgcgccgca ggatgcggtc gttctggtag ttggccacgt ggaggacgac
     4801 ggcgtcgccg ggcttgagcg agttgacgaa gccctgaaag tcggcgagag agttgacggc
     4861 cacgcggtta accctgagga tgaccacccc ttcatcgagg cccgagtcgt cggccagact
     4921 gccgtcctca acgtccgtga taatcacgcc gcgcaggccg cgcaggttct tctcctcggc
     4981 gagctggtcg gtcagctcgc tgaccttcag gccgagcgtg ggccggtcgg tcggcgcggc
     5041 cgacgacttc ttcttcttcg cgtccggctc ctctttgcgg tcgccctgaa gggtgcggct
     5101 gggcgggcgc tcgccgacgg tgacggcggc cgtgcgtcgc tccaacttgt tgtttagctc
     5161 gcgcaggtag gccacctgaa cggcgcggcc cacctcggtg gccgcaactt tgttgatgag
     5221 gtcttgcgcg tccgtgacgg gtcggccgtc gaactcgacg atgatgtcgt tggtcagaat
     5281 gccggccttg gcggccgggc cgttctggtc ggccacgtcg cggacgatgg cgcccttcgc
     5341 ctcggcgagt ccgtagacct gggcgaactc ggcgcgcacg ggctggagat agataccgag
     5401 gtagccgcgc ctcaccttgc cgccgccgac gatttgtttg tagacgaagg cggcctcgtt
     5461 ggcggggagc gcgaaaccga tgccgttgta gtcgccggtc gaggtggcga tctgcgagtt
     5521 gacgccgatg acctcgccgc gcatgttgac gagggggccg ccggagttgc cgcggttgat
     5581 ggcggcgtcg gtctggagga aacgctggaa ggcggccgag gggtcggtct ggcgctcgcg
     5641 ggtcgagatg atgccggccg tgacggtctg ctcaaggccg aagggcgagc cgatggccag
     5701 cacccagtcg ccgacctgca cgtcatcgga gttgccgaac tgcacggccg gcaagtcctg
     5761 cggcggcctg acgcggacga tggcgaggtc ggtctcctcg tcgaagccgg ccacctcgcc
     5821 gcgcaaggtc gcgcccgact ggagcttgac gttaattttg ctggcgccct gaacgacgtg
     5881 gtagttggtg agaatcagcc ccttggggtc gatgacgaag ccggagccga cgccgcgcga
     5941 gggccggcgc atccgccgcc ggagcatgtc gagcagcgga ttgctgttgc cgcccggcgc
     6001 ctccttctcg gcctcgtcct cctcttcgtc gtcgtccttc gcggcgatct gcggcgcgga
     6061 gacggtgtcg atgttgacga cggccggctc gacgcggcgg gcaatctcgg cgaaggaggc
     6121 cgacagagcc tcgggcgcgc gcgcaatctg ggcaatcggc gcctcgttct gcgcgacggc
     6181 ctgcttcgac gcgagcatcg cgcccaagcc cagaccgacg agcagacaga gcgcggcgag
     6241 cgcggcgatc cacacgcgga gccggcgcag catggcctgc tccgccctgt tcgtctgacc
     6301 ctttgaggct tccgtcataa agcttgtacc accttcggga aagagccttg ctgtcttacg
     6361 cggcacacgc gcctgaaaga cgctaccgcg cccccgtctc ctgccgccgg tctggggatt
     6421 actcaaagaa gaagcggggc tgtgagtata acgaaacccc gccgacgccg caaagcaagt
     6481 ttgtaaaagg gctgtaaaag cttcaacgcc gcagcgccca cggccggggc ttttattctt
     6541 gagggctttc acattgagcc gtaggtgtta gctgccgctc ctcacgtttc cgtccgcccc
     6601 gccgcccgcc gctcgcccca gcgcaactcc gagacgatga taccggcgag gatgagcgcc
     6661 gcgccgaggt tggcccgccc gcccgtccac tcgcccgcgc cgcgcatcca gacggcgaag
     6721 atggtggcga agaccggctc cagactgaag atgatggccg cgtgcgtggc cgacatccgc
     6781 ccc
//
LOCUS       DASVES010000168         6953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_693887, whole genome shotgun sequence.
ACCESSION   DASVES010000168 DASVES010000000
VERSION     DASVES010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693887"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF240_06755"
     CDS             complement(<1..491)
                     /locus_tag="VF240_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174963.1"
                     /translation="MADTPETDKLVKKEPEAPAPEAPARDPITSRTTSSILLISALLL
                     TGTLAWALWDEVYGQRPWKDTQASFVKRYNRYLKRLQRSGFQSEQQVRESAEYQDLDQ
                     GARAAEELIKPELQVIDRKVALIDDQLTVISDEFQDRRGRITVKNYQIESADAGDKAE
                     MRQ"
     gene            complement(518..1639)
                     /locus_tag="VF240_06760"
     CDS             complement(518..1639)
                     /locus_tag="VF240_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174964.1"
                     /translation="MAAVMTDESRPRRGRGALVALAVFIVLIALSALFVEDWGQLWTI
                     ISTPDNVPIAGILVLVPFFTWLGFKQASDNDRLVEKLEADPALAKTHHRKALPWRPGW
                     ARELHVWPFLIRIEFLAAVIVTVILFIWSITLNAPLEEPANPNLTMNPSKAPWYFLGL
                     QEMLVYFDPWIAGVVMPGLLVVGLMVFPYVDSNPLGNGYYSWKQRRFAIGMFTWGFVM
                     WLLLIMIGTFIRGPGWIWFWPGQRWDHNAVVFDRNRDLHDIVAGWGLPFLSQAPFREI
                     FGALVVGLLLLLGGLFFHWLMLRGPLRGEHFKSFRRFKEWATVSDPFEKKLLQRTSLL
                     QYVTFQFFAISVLWLFPVKLLLRLLFTIKYIWVTPWFNV"
     gene            complement(1800..2750)
                     /locus_tag="VF240_06765"
     CDS             complement(1800..2750)
                     /locus_tag="VF240_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX7174965.1"
                     /translation="MDPERPESKNGDEKTPAPAPAATGLVERARGQGLALKEQGVALS
                     ERAKKEMEALKDPQQTQLWKSIFRVTHDRGDPRNRSLAVLSNVFLHLHPAKINRDAVR
                     YNFTWGMGGITFYLFIVLTFTGVLLMFYYHPTKVQAFRDILYLEHDVPFGKLLRNMHR
                     WAAHLMVITTWLHMFRVVLTGSYKRPREFNWCVGVVLLVLTLLLSFTGYLLPDDQLGF
                     WAVTVGTNMARATPLLGSEGPFGPQLGITAFNDVRFGLLGGSIVDANALLRSYIWHCI
                     GIPLVASIFMAVHFWRIRKDGGISGPAPVMLEAEMKAVKK"
     gene            complement(2832..3791)
                     /locus_tag="VF240_06770"
     CDS             complement(2832..3791)
                     /locus_tag="VF240_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7174966.1"
                     /translation="MDSEKNPTPPAGSASDGEKATDASPAPVSPATQQNVDELLDAAR
                     GEVKPRPASASARTPVTPSAKEASANTRAEAAPTGDAPGKETPATTPAAARAAPDTGA
                     SKTTSTPTPSINKGVSEGVAAKVPATTAKPAAATAKSAVAKPPAGAVVTAPVADGDEA
                     IFKMRRRIIWASVAGFLAASFLMFLRFFLPRSITEPSNVVRLGFPGDYNFGVDTKWQQ
                     QYRIWVVKNSERLFVISAICTHLGCTPDWKESENKFKCPCHGSGYDSEGINFEGPAPR
                     PMDRAFVELDAEGQIVTDKGRLFQWPKGGKNGFNDDGSFIRIA"
     gene            4087..4338
                     /locus_tag="VF240_06775"
     CDS             4087..4338
                     /locus_tag="VF240_06775"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX7174967.1"
                     /translation="MTTRPHVIFYTRPGCHLCEDAKLAIAAADCAGSYTFEEVNIEAD
                     PDLRRRYGWDIPVVVINGRVVFKHRLSASDFKRALSGKL"
     gene            complement(4459..6840)
                     /locus_tag="VF240_06780"
     CDS             complement(4459..6840)
                     /locus_tag="VF240_06780"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7174968.1"
                     /translation="MSRRSGVLLSVLLAALALTGAQFSSGAQTEDAALTSAAELPTLA
                     ITSPISGFNQPVVITHAGDASNRLFVVEQVGRIRIVKNGALLPASFLDISTRISSGGE
                     RGLLGLAFPPGYAAKGYFYINYTNPAGDTVVSRFRRSAANADAADPASEQVVLTIAQP
                     FPNHNGGQLAFGPNDGMLYIGMGDGGSGGDPGNRAQNPNELLGKVLRIDTETGAPLTY
                     TVPPGNPFVNRTGFRPEIWALGLRNPWRFSFDRLSGDLYVADVGQQNWEEVNFQPASS
                     AGGENYGWRIMEGTHCFDPNIICSTFGLTMPVAEYDHTQGCSVTGGYVYRATTFPRMH
                     GLYFYGDYCSGRIWGLRREAGQWQNTLLLDTSINISTFGEDEAGNLYVANHGAGQVFR
                     LVDTAPPPTPTPTPTPTTGPPTLQFSQASLSVTENCTGAAFTVTRTGTTSAAVSVEYV
                     SSDGTAKQKSDYTYAAGRLNFAAGETSKTFNVSISEDALDETDETATLSLLNPSSGAV
                     LGAQSTATLVIEDEDVGDTASNPIEDPETFVCQHYHDFLNREPDAEGLNFWTSQIVSA
                     VCGSDRACLIDRMENVSAAFFLSIEFQNTGYLVYRYEKASFNRPPRYTDFLRDTRRIS
                     EGVVVNQGDWAERLEQNKQTFTDDWVARPEFRALHDGKTNAEYVNALFANSEVAPTAA
                     ERDALVSGLNAGTETRATVLRKVAQNNAVFNKQYNSAFVLIQYFGYMRRNPDDAPDTD
                     LTGYNFWLRKLDEFTLPGEDAGVESVALGRIRRAQMIRAFIVSGEYRERFGRP"
BASE COUNT         1241 a         2112 c         2294 g         1306 t
ORIGIN      
        1 tcctgacgca tctccgcttt gtcgcccgcg tcggccgact cgatttgata gttcttgacg
       61 gtgatgcgcc cgcgcctgtc ctgaaactcg tcggagatga ccgtcagttg gtcgtcgatg
      121 agcgcaacct tgcggtcgat gacctgcaac tccggcttaa tcaactcctc ggcggcgcgc
      181 gcgccctgat ccaaatcctg atactcggcg ctttccctga cctgttgctc ggactggaag
      241 ccgcttcgct ggagacgctt cagatagcgg ttgtaccgct tgacgaagct cgcctgcgtg
      301 tccttccagg ggcgctgtcc gtagacctcg tcccagagcg cccaggcgag agtgcccgtg
      361 agcaggagcg cgctgatgag gaggatactg ctcgtcgtgc gcgacgtaat ggggtcgcgc
      421 gccggggcct cgggcgcggg cgcctccggc tccttcttga cgagtttgtc ggtctcaggg
      481 gtgtcagcca cgtcgcattc ctccgctcga ttcagcctca gacgttgaac cagggcgtga
      541 cccagatgta cttaatcgtg aagagcagcc gcaacaggag cttgaccggg aacagccaca
      601 gcaccgagat ggcgaagaac tgaaacgtca cgtattgcag caggctggtg cgctggagaa
      661 gcttcttctc gaatgggtcg gacactgtcg cccactcttt gaatcgccgg aacgacttga
      721 aatgctcgcc ccgcagcggc ccccgcagca tcagccagtg aaagaacagc ccgcccaaca
      781 ggagcagcag gccgacgacg agcgcgccga agatttcgcg gaagggtgcc tgcgacagga
      841 acggcagccc ccagccggcg acgatatcgt ggaggtcgcg gttacggtcg aagacgacgg
      901 cgttgtgatc ccaacgctgc cccggccaga accaaatcca gcccggcccc cggatgaagg
      961 tgccgatcat gatgaggagg agccacatca cgaaccccca tgtgaacatc ccgatagcga
     1021 agcgcctctg cttccaactg tagtagccgt tgccgagcgg gttcgagtca acgtagggga
     1081 agaccatcag cccgacgacc aaaagccccg gcatgacgac gccggcgatc cacgggtcga
     1141 agtagacgag catctcctga aggccgagga agtaccacgg cgccttcgag ggattcatcg
     1201 tcaggttcgg gttggccggc tcttcgagcg gcgcgttcag cgtgatcgac cagatgaaca
     1261 ggatgacggt gacgatgacg gcggccagaa attcgatgcg aataagaaac ggccaaacgt
     1321 gcagctcgcg cgcccagccg ggccgccacg gcagcgcctt gcggtggtga gtcttggcga
     1381 gcgccgggtc ggcttcgagt ttctcgacga ggcggtcgtt atcgctcgcc tgcttgaatc
     1441 caagccacgt aaagaacggg acgagcacca gaatcccggc gatgggcacg ttgtccggcg
     1501 tcgagataat cgtccagagc tgtccccaat cctcgacgaa gagggcgctc agcgcgatca
     1561 ggacgataaa cacggcgagc gcgacaagtg cgccgcgtcc ccgccggggc ctgctttcat
     1621 cggtcatcac cgcggccatg acttaagtcc tctcggagtg gctgttagct gttagcgatt
     1681 agctgttagc cagcagcagc tcatgcttga agccgtcagg agcgtcgtct tcaaatggcg
     1741 aacgccctcg tccgccatct tttagctaaa agctaacagc ttatagctaa ttgctttctt
     1801 cacttcttca ccgccttcat ctctgcttct aacatcacag gggcagggcc ggagatgccg
     1861 ccgtccttgc ggatgcgcca gaagtggacg gccatgaaga tggaggcgac aagcggaatg
     1921 ccgatgcagt gccagatgta ggagcgcaga agggcgttgg cgtcaacgat ggagccgccc
     1981 aagaggccga agcgcacgtc gttgaaggcc gtgatgccaa gctgcgggcc gaacgggcct
     2041 tcgctgccga gcagcggggt ggcgcgcgcc atgttcgtcc ccaccgtcac cgcccagaat
     2101 ccgagctggt cgtccggcag tagatagccc gtgaacgaga gcaacagagt cagcaccagc
     2161 agcacgacgc cgacgcacca gttgaactcg cgcggccgct tgtaggagcc ggtgaggacg
     2221 acgcggaaca tgtgcagcca cgtcgtaatc accatcaggt gcgcggccca gcggtgcatg
     2281 ttccgcagga gcttgccaaa aggcacgtcg tgttcgagat agaggatgtc gcggaacgcc
     2341 tgcaccttcg tcgggtggta gtagaacatc aacagcaccc cggtgaaggt cagcacgatg
     2401 aagaggtaga aggtgatgcc gcccatgccc cacgtgaagt tgtagcggac ggcgtcgcga
     2461 ttgattttcg ccgggtggag gtggagaaag acgttcgaga ggacggccag agagcggttg
     2521 cgcgggtcgc cgcggtcgtg cgtgacgcgg aagatggatt tccagagctg tgtctgctgc
     2581 gggtccttga gggcctccat ctccttcttg gcgcgctcgc tcaatgcgac gccctgctcc
     2641 ttgagcgcga ggccctgccc gcgcgccctc tcgaccagac ccgtcgcggc cggggccggg
     2701 gccggagtct tctcgtcgcc gtttttggat tcagggcgtt cgggatccac tcgattgcct
     2761 ccccttcgtg ctaaacgtca aatcgacgcg agcgacgctt ccctcttgaa aacctccagc
     2821 cggaaaaact gttacgcgat gcggatgaat gagccgtcgt cgttgaaccc attcttcccg
     2881 cccttgggcc actggaagag ccgccccttg tcggtgacga tctggccctc ggcgtcgagt
     2941 tccacgaaag ctctgtccat cggtcgcggc gcaggcccct cgaagttgat tccctcgctg
     3001 tcgtagccgc tgccgtggca ggggcacttg aacttgttct cgctttcctt ccaatcggga
     3061 gtgcagccga ggtgcgtgca gatggcgctg atgacgaaca gccgctcgga gtttttgacc
     3121 acccagatgc ggtactgctg ctgccacttg gtgtccacgc cgaagttgta gtcgccgggg
     3181 aagccgaggc ggacgacgtt cgacggctcg gtgatcgagc gcgggaggaa gaagcggagg
     3241 aacatcagaa agctcgcggc caggaatccg gccacgctcg cccagatgat ccggcggcgc
     3301 atcttgaaga tcgcctcgtc gccatcggcc acgggcgctg tcacgacggc gccggcgggc
     3361 ggtttggcca ccgcggattt ggctgtcgcc gcggccggtt tggctgtggt cgcgggcact
     3421 ttcgccgcga cgccctccga gacgcctttg ttgatcgaag gcgtgggcgt ggaggtcgtc
     3481 ttcgacgcgc cggtgtcggg tgcggcgcgc gccgcggccg gtgtggtggc gggagtctcc
     3541 ttgccggggg cgtcgcccgt gggcgcggcc tccgcgcggg tgttggcgga tgcttctttg
     3601 gccgacgggg tgacgggcgt ccgcgcggac gcggaggcgg ggcggggctt cacttcgccg
     3661 cgggcggcgt cgaggagttc atctacgttt tgctgtgtgg cgggcgagac tggcgcgggc
     3721 gacgcgtccg tcgccttctc gccgtcggat gcacttccgg cgggcggtgt tggatttttt
     3781 tcgctatcca cgccgcgacc tccctctcag tgttttggct tgcgagtggc ccaaagtgtc
     3841 gtcggatcag tcatcgaacc gcctgggccg gggacgtcac gcgcaggcga tacaagtcac
     3901 cgaaaaagag gcgaggcctc tttaacagcc cttcttattc aattgatacg cgtgacatga
     3961 agtgcgccgt gcccgaaaaa gataatagaa tgactcggct tgcggcattt atactatttt
     4021 cgggcggccc gtggcaagat gttatttcaa aaacgggccg cacaccagca ccaacctatt
     4081 ggggccatga ccaccagacc acacgtcatc ttctacaccc ggcccggctg ccacctctgc
     4141 gaagacgcga agctcgccat agccgcggcc gactgcgccg gctcctacac ctttgaagag
     4201 gtcaacatcg aggccgaccc cgacctccgg cgacgctacg gctgggacat tcccgttgtc
     4261 gtcattaacg gccgcgtcgt cttcaagcac cgtctgtccg ccagcgactt caagcgcgcc
     4321 ctgtcaggca aactttaaga ggcggtatcg gcaacagtac cgcgagcggg tgcgcctgcg
     4381 atgagaggcc cgcctcgtgc atccagaccc gctcgctacc gctcgcggta ctgtgttagc
     4441 ccgctgtctt cccgccggtc acggtctccc aaatctctcc ctgtactcgc ctgagacgat
     4501 gaacgccctg atcatctgtg cgcgcctgat cctgccgagc gccacgctct cgacgcccgc
     4561 gtcctcaccc ggcaacgtga actcatctaa ctttcgcagc cagaagttgt agccggtcaa
     4621 atccgtgtcg ggcgcatcgt cgggattcct cctcatgtag ccgaagtact ggatgaggac
     4681 gaaggccgag ttgtactgct tgttgaagac agcattattc tgcgccacct ttcgcagcac
     4741 cgtggcgcgc gtctccgtcc ccgcgttgag gccggacact aacgcgtcgc gctcggccgc
     4801 cgtgggggcg acctccgaat tggcgaagag cgcattaacg tactcggcgt tggtcttccc
     4861 gtcgtggagc gcccggaact cgggccgcgc gacccagtcg tccgtaaacg tctgcttgtt
     4921 ctgctccaga cgctcagccc agtcgccctg attgacgacg acgccctcgc tgatgcggcg
     4981 tgtgtcgcgc aggaaatcgg tgtagcgcgg cgggcggttg aacgacgcct tctcgtaacg
     5041 gtagacgagg tagcccgtgt tctgaaattc gatggagagg aagaaggcgg ccgagacgtt
     5101 ctccatgcgg tcgatgaggc aggcgcggtc ggagccgcag acggccgaga cgatctgcga
     5161 agtccagaag ttgagacctt cggcgtccgg ctcgcggttg aggaagtcgt gatagtgctg
     5221 gcagacgaag gtctcggggt cttcgatggg gttggacgcc gtgtcgccaa cgtcctcgtc
     5281 ttcgatgacg agcgtcgccg tgctctgcgc gccgaggacg gccccgctcg aagggttcaa
     5341 tagcgacagg gtcgccgtct cgtccgtctc gtcgagcgcg tcttcggaga tcgagacgtt
     5401 gaaagtcttg ctcgtctcgc cggcggcgaa gttgagacgc ccggccgcgt aggtgtagtc
     5461 gctcttctgc ttcgccgtgc cgtcgctcga cacgtattcg acgctcacgg ccgctgacgt
     5521 tgtccccgtt cgcgtgacgg tgaaggcggc gcccgtacaa ttttctgtga ctgagaggct
     5581 ggcctggctg aactgtaaag tcggcgggcc ggttgtcggc gtgggcgtgg gcgtcggcgt
     5641 gggcggtggg gcggtgtcaa cgagtcggaa gacctgaccc gcgccgtggt tggcgacgta
     5701 gaggttgccg gcttcgtctt ctccgaaggt ggagatgttg atggaggtgt cgagcagaag
     5761 cgtgttctgc cactgcccgg cctcacgccg gaggccccag atgcggcccg agcagtagtc
     5821 gccgtagaaa tagaggccgt gcatgcgcgg gaaggtcgtg gcgcggtaaa cgtagccgcc
     5881 ggtcaccgag cacccctgcg tgtggtcgta ctcggcgacg ggcatcgtca gaccaaacgt
     5941 cgaacaaatg atattcgggt cgaaacagtg tgtgccttcc attattcgcc atccgtaatt
     6001 ctcaccgccg gcgctcgatg cgggctggaa gttcacctcc tcccaattct gctgccccac
     6061 gtcggccacg tagaggtcgc cggagagccg gtcgaacgaa aagcgccagg gattacgcaa
     6121 accgagcgcc cagatttcgg ggcggaagcc cgtgcggttg acgaaggggt tgccgggcgg
     6181 gacggtgtaa gtgaggggcg cgccggtctc ggtgtcgata cgaagcacct tgccgagtag
     6241 ctcattgggg ttctgcgcgc ggttgccggg gtcgccgccc gagccgccgt cgcccatgcc
     6301 gatgtagagc atcccgtcgt tgggaccgaa ggcgagctgg ccgccgttgt gattcgggaa
     6361 gggctgcgcg atggtaagga cgacctgctc gctcgcgggg tcagccgcgt ctgcattggc
     6421 ggcgctgcgg cggaagcggg agacgacggt gtcgccggcg gggttcgtgt agttgatgta
     6481 gaagtagccc ttcgcggcgt accccggcgg gaaggcgagg ccgaggaggc cgcgctcgcc
     6541 gcccgaactg atgcgcgtcg agatgtcgag gaaggacgcg ggcaggagcg cgccgttttt
     6601 gacgatgcgg atacgcccca cctgttcgac cacgaagagg cggttgctcg cgtcgcccgc
     6661 gtgcgtgatg acgacgggct ggttgaagcc ggagatggga gaagtgatgg cgagcgtcgg
     6721 aagctcggct gcgctcgtca gcgcggcgtc ctcggtttgt gcgcccgacg aaaactgcgc
     6781 ccccgtcaaa gccaacgccg ccagaagcac tgacagcaac actcccgacc ttctcgacat
     6841 agacagactc ctcccttaaa cgtttttgcg ccgggtgtga tattgggaaa actggggaga
     6901 cgagacctgt ggagtataga acttttgcgg tctttaagaa actgcctttt gtc
//
LOCUS       DASVES010000169        18241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_184528, whole genome shotgun sequence.
ACCESSION   DASVES010000169 DASVES010000000
VERSION     DASVES010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18241
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184528"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..282
                     /locus_tag="VF240_06785"
     CDS             25..282
                     /locus_tag="VF240_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174969.1"
                     /translation="MTISSHPHFLNRFGYIPRKIAAGTLDIARELKSGLASVSVGRRV
                     RFQLAANDKFLRNGSQRFSKTMPAAHRMTVSSNNAGDGVEK"
     gene            572..766
                     /locus_tag="VF240_06790"
     CDS             572..766
                     /locus_tag="VF240_06790"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174970.1"
                     /translation="MPTGTCPECDADVHVDIDSDKGDAVTCDECGTHLEVVGLDPVEL
                     DIAEDDDDSGYDEDDDEDEY"
     gene            complement(854..1813)
                     /locus_tag="VF240_06795"
     CDS             complement(854..1813)
                     /locus_tag="VF240_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7174971.1"
                     /translation="MPNAQQKPPARDAGMPPPAGQEIDADEVLRIDTDLVLVDVLVSD
                     AEGRPVRGLRPEDFKIYEDGVERPVAFFNVERRGGAERPVAVVFLIDVSGSMTAGEME
                     RVRMALDEFVRGMSARPASFALMGFGMSAKVYQNFTADRAKLTRSFTRLAREMNGLST
                     HTYDAVDDAVRLLVRNAPRTRDRQVVKRAVVVITDGFPVGDTVRPETVIERANAAGVS
                     VYSVTLPSYSRAMASVGGPLPTPLDVSGLAERTGGASVYANREDYGPLFRELAEEVTS
                     TYVLAFYPPEEKRRDGRHHTVRVEAPRGLSVRQSRGGYKSSDK"
     gene            2129..2773
                     /locus_tag="VF240_06800"
     CDS             2129..2773
                     /locus_tag="VF240_06800"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7174972.1"
                     /translation="MNIKSITRLFLSLLLLSAATAVAAPAQQQDKTTGGVKGKVRVET
                     GSSAGVTVVVRRGDDEVRSATTDGRGEFEVRGLKPGSYGLTFRKTGLKVGKVEGVEVR
                     AGKVRSLGGGLYMSMDEGALAIIQGSVFNQDGKSLTGAKVELALLSPDGAAKKLESRL
                     SSIETGRFVFKLTPEAARYRLTATAPGRQPATQEVTVDGAAIYRVALSLAPAAK"
     gene            2797..3063
                     /locus_tag="VF240_06805"
     CDS             2797..3063
                     /locus_tag="VF240_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7174973.1"
                     /translation="MKDEAHKDGETRTEFLTARQLGAVLQVSESTVRRLAHSGRIPSI
                     RITPHIIRFRLAAVVEALDGARRSRRSTRAEDNGADDSQLSFFE"
     gene            complement(3169..3753)
                     /locus_tag="VF240_06810"
     CDS             complement(3169..3753)
                     /locus_tag="VF240_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174974.1"
                     /translation="MIQRKRLAVIKRAGRVALAVLTIQLVGASAAQARGAAQGGVEGE
                     ARAALQQAFELLRAGDYGGVYDALPSASQRRVTRARFVEALGKARGHFELNGMEIERA
                     HVAGNLAVMDTVVYATVRKPMEAEGKIVSRQYMVREGGRWRVTTGDGSTVRPLLAANP
                     AFARQYPATEPRVYLKRDGRWINLSALQGSRRRK"
     gene            complement(3722..3994)
                     /locus_tag="VF240_06815"
     CDS             complement(3722..3994)
                     /locus_tag="VF240_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174975.1"
                     /translation="MEGFTEKAGADVTARQRWSVAAMLCLVAGGALFLLGRQDWAFAA
                     AVLGVVAWFLNVRAQLHRANVGRDEERARVEDEDADDDTEEEAGGY"
     gene            complement(4015..4515)
                     /locus_tag="VF240_06820"
     CDS             complement(4015..4515)
                     /locus_tag="VF240_06820"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD N-terminal
                     domain-containing protein"
                     /protein_id="HEX7174976.1"
                     /translation="MTFLASCGGAGEGNRNATPANAARTAEQASAPGADAVRASVSEV
                     KLAAGGSGEAVVRLNIADGFHVNSNAPGDKFVIPTRLEATPAAGINAGTAVYPEGVKK
                     KFPFSEQPLSVYEGEVAIRLPLSADAGASKGRHTVSADLTVQPCDDRECFPPRKINAA
                     VPVVVE"
     gene            complement(4917..5876)
                     /locus_tag="VF240_06825"
     CDS             complement(4917..5876)
                     /locus_tag="VF240_06825"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX7174977.1"
                     /translation="MRLKTALSSVLILLALAGCSRSEGGANSASRPAAPDSGALTTSG
                     GKAERSSAGATAEAEIRDVSLSQADASQAASVPTERKIIRNAALTIESDAPAEGQRRI
                     ASIAEQRGGFVVTSESRHQDGDGERGYEVVTMEVRIPFAQFDAVLGEIRGVGSAVVDE
                     KITGQDVTEEYIDLEARARTQRAFEAQLLELLKRAGGVTEALEVQRALATVRTEIERL
                     EGRRRFLENQSSLSTIKVTLRPAGVLAGASTRGFFPGLKRAFANGIDVALSITLGLIQ
                     VIIALIPVFLLIVLPLMLLFRFSMRRRRRNAPPPPTAAAPPVG"
     gene            complement(6022..6642)
                     /locus_tag="VF240_06830"
     CDS             complement(6022..6642)
                     /locus_tag="VF240_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX7174978.1"
                     /translation="MNVIAILGTALGAAWTSGINLYATVAVLGLLQHYKFAQLPGGLE
                     VLDNWWIIGVALGLYVVEFVADKVPYVDSVWDVVHTFIRVPAGAALALAATTELAPAV
                     QVVALLLGGTLALSTHGTKATVRAAANTSPEPVTNWALSLAEDVVAVGAIVLAVLHPL
                     VILAVIFVFLLLLVWLLPKVLRRLRKMLASARGLFGGRARAESARR"
     gene            complement(6665..6934)
                     /locus_tag="VF240_06835"
     CDS             complement(6665..6934)
                     /locus_tag="VF240_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174979.1"
                     /translation="MTDEEINKKFDVVAEHLATLAVGQQRLQETQAQTEQIVNRLAAA
                     TLEGFRDVNAKINALVDAQMRTEENLKNLTAVVDRYFSEGRNGRS"
     gene            complement(6975..8336)
                     /locus_tag="VF240_06840"
     CDS             complement(6975..8336)
                     /locus_tag="VF240_06840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7174980.1"
                     /translation="MARKSILVVDDEKSQREILEMILSEAGYDVTTASSGEAALRIAR
                     ERRFDLALTDLKMTGMDGIELLSKLLAYDSSIIVILLTAHGSVDSAKEALRRGAFDYL
                     EKPYDRETLLETIQRALARLDALDTEIMSVSGEMEAVKKMILKVARSNSTVLIRGESG
                     TGKELISRAIHTQSLRADQMFQAVNCAAINENLLESELFGHERGSFTGAHAEKKGLFE
                     VADKGTLFLDEIGELDVSMQAKLLRALQEREIRRVGGTRAIKIDVRVVAATNRDLRAM
                     VSDGRFRDDLYYRINVLSIDVPPLRERREDIDLLIDYFIKKHTRNTSRLVRGITPDAR
                     LLMNDYSWPGNVRQLESAIERAILLAESDLITIEDLPLEVRQEARPSPEGGFKLPAEG
                     LVFEDMERQLILQAMERTDYNITKAAKLLGLTFRTLQYRLEKFGIKRADKHGGDEEEE
                     SAE"
     gene            complement(8394..9956)
                     /locus_tag="VF240_06845"
     CDS             complement(8394..9956)
                     /locus_tag="VF240_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7174981.1"
                     /translation="MKLPNLFYSFRMRLLLVLALLLFATLGVQYYLFYREEMRRAAII
                     SEQEQALAASTALALESITTPTRQYLWEMDETRPVSFLQEQAGRVQNVLVINEQGRVD
                     DSLDPQFAPRTVNGEARYYHVSELNLPRLVVAGPVTEDLMRLINAPEGSAATTTPIAG
                     EPRAFTMQVRLIDEEGGPTVNYIIVVLGATKTPQNDSWEDILRPLLPTLAVLLLATTA
                     AAILVWRFTRPIKALSRATQRVASGHFDFRVPGGHRRDETGVLVSTFNEMIAGLSHTR
                     ELEVKVKQAEQSAVVGRLASAIAHEVRNPLNYINLTLDHLRTSLAPEDPQKRELFGRL
                     ANQLKAEVARINTRITEFLKYSRPAALDLKPIDLGALLDDALRMVEVQAEETGVRTAV
                     EREGGALEVLGDRESLRSVFTNLIINGMQAMGSEGGSLTIRLSSDDGVVRVGVSDTGR
                     GIPPDNLPKIFEPYFSTKETGTGLGLAIVKKAVDDHGGHITVESKQDEGTTFTVTLPI
                     GERGQRSEVRGQ"
     gene            10392..12170
                     /locus_tag="VF240_06850"
     CDS             10392..12170
                     /locus_tag="VF240_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7174982.1"
                     /translation="MSPASALAILFIFISLAVVPVAASAKKTPPKKTPAPKAAARKSA
                     RDDRRGREKASSKREVAQKNSRQRDARASKNERAGRGRDRASKNDRASKNERASKNER
                     ASKNDRRLSRAERREQARESRKDSKRMSRREREAAAREQARRRREAEERARQAAIARA
                     IYLARIRAQDQAWRDETQANIRRDETTGEDPEVRRAAIEALGNNAGSVVVMEPKSGRV
                     LAVVNQDWGLRRGFKPCSTVKLVTGLAGLSDNVIDPVQTVNTSAGTLSIDLTDSLAYS
                     NNHYFQRVGGQVGFDRMMHYARELGLGQPTGINHPGESAGRLPLIKEGFSVNRMSSHG
                     DDIEVTPIQLANMASAIANGGERLVPHLPRTPAETTRFERVVRSKVNVPQEHLRRMLP
                     GMIGAVNYGSGRTAYDPAQTIAGKTGTCISQDAARTWLGLFTSFAPVHDPRLAVAVVT
                     RGSSQRGKIAAGIAGRVYRSLSHRYGPRAGDRPMLAEETLIPRPKVDPSKAAVLNDEE
                     AEAEAESNAVNSDAYVVKEAGNVGVRQVFEGQQNNVKRTAKIYEKPAVTSAPVTVAPA
                     PAQTNAPAGATRPRRVGAEGQPQQQQ"
     gene            12559..13767
                     /locus_tag="VF240_06855"
     CDS             12559..13767
                     /locus_tag="VF240_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174983.1"
                     /translation="MKNFLRFMTLNAIVAACALSASAQVQPAQPNATPDADAAAKAAC
                     TDLYTKWRDNYKGDAAKQKIAYEVGKQFVTQCPSDEYLSYVQKWIPKYEAAMQGDQLQ
                     KQYDDCVKASDWKCILNSGKQLAAKDPENLTLYLNLTAAGSSSKDKTLFAESLNAAGR
                     TIQLVEAGKADAFTPEMWKGIPFASNKAELLGFLNNLSAFYSLDTKPAEAAKYALKAV
                     QSEGRYKKDPAGYYYLSLAYQAAEYAPQAQAYKAACEGKELNDECKMKLDTLYLVVDR
                     IIDAHARLVSLTTDATAKAARLKEREGFYRFRNEDKIDGMNELVAGIQAKPLLLPSMQ
                     TMPTPAPAPSTTTTTPSATTSNPGTATTTPAATTTTTPAPANAAKPATTSTTTPTPKP
                     TNGTAAKPKP"
     gene            13927..14715
                     /gene="rnc"
                     /locus_tag="VF240_06860"
     CDS             13927..14715
                     /gene="rnc"
                     /locus_tag="VF240_06860"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX7174984.1"
                     /translation="MTDDTPTDTATDFDALEQALDYDFENRALLERALTHRSWAHEHV
                     GPGEEARARQLHNEAMEFVGDSVLGLVIADALFHSHPEVTEGELSRMKHRLVSTQTLA
                     RVARRMGMGEHLRVGRGEEKTGGRRKRALLADVFEAVLAAVYLDRGLEAARAFVLRAL
                     EEEIEQASPERAAALDTKTLLQERLQAERHVAPVYNLIETEGPPHHRVFHVEVCWDER
                     AVRGEGGTIKAAEAEAARRAIEQMGAAPTGEAAAPEAVRDEAAD"
     gene            14794..15753
                     /gene="lepB"
                     /locus_tag="VF240_06865"
     CDS             14794..15753
                     /gene="lepB"
                     /locus_tag="VF240_06865"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7174985.1"
                     /translation="MDNEQTGTGVAEPPPASAGDPYANSAGASEERRPLASAPEETRP
                     PQGPPRGVLREYFESLVVTAIMALFGMTFVVQAVKVPTGSMQNTILIGDHLLVNKFIF
                     APGPPVFFLPQREIRRGDIVVFKYPGKSKDPQFERHFIDDTRPDNIPFKINYVKRVVG
                     LPGDVLELRGSQVLINGQPLDERRVPATNPPDDDDERTSGDESGAPLGVAADIPAVGE
                     GPYEVYWRPDRFRASRAATTTAGEKFAVPEEHYFVMGDNRENSQDSRYWGYVHRDLVI
                     GRAMFVYWSFDESLPRSEAPFPLNALSDFVSNSRWGRTGTLVK"
     gene            15827..17056
                     /locus_tag="VF240_06870"
     CDS             15827..17056
                     /locus_tag="VF240_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX7174986.1"
                     /translation="MIKALSLGGLSWAELGKRVWREIEEDEVFGRAAQLSYYFLLALF
                     PLLLFLTSILGLIADENGELRKSLFEYLGSVMPGEASKLVSDVIDGSGGGKLSFGILA
                     TLWAASNGMGAILEALNVAYEVKETRPWWKARIVAIGLTFALALLILSALVLVLYGHN
                     IAETVAGSFGLGRAFELGWKVLQWPIVLAFVLLAFALLYYFAPDLHDQDWKWVSPGAV
                     LGVALWLIVSFAFRTYLQYFNTYSATYGSLGAVIIMMLWFYFTGLAILVGGEINSEIE
                     NEMAKQGAPDAKEKGEKAPDDGKPEAAASAEGTRGKASADATGGTAVARKTDAVAVKK
                     PQKQTEKAGRSAQSSVPARRGRDSGAGLTSNASQGRSRLNSEEMRTAKRTARSFSLGK
                     AVVVLGAWVVSKVRGKR"
     gene            complement(17070..>18241)
                     /locus_tag="VF240_06875"
     CDS             complement(17070..>18241)
                     /locus_tag="VF240_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7174987.1"
                     /translation="TIEQAKDLLDRPAVKAAFAHVDRERAETLREWVAITEINAPSGK
                     ERERALYVEKLLRASASLEVRSDSAGNLVATRKGTGGGPLVVIDAHLDTVFQEGLKIK
                     AEVRGGRVYAPGVGDDTRNIEAMLATIRALDAANVKTKGDLVFVFTVEEETTLRGAEE
                     YVKDNKGRIDFYIALDGGYEGFTYGGIGINWYRHHLIGPGGHTRSRNPPYSAALPLAR
                     AVRRIYELELPENPPTHVNIGMLGGAEVVNAKAADAWFTVDLRSTSNEVIGELEKKIA
                     DVVREEAEREKMTVKTEVISKTPAAQIPGLRESPFVKLMEAVHRELGFEGAITMTASN
                     NSIAALLAGIPSISTGAGPCGDSHALTEWCEIEPLYRGIKKLLVMEVAAAQLSTR"
BASE COUNT         3234 a         6077 c         5915 g         3015 t
ORIGIN      
        1 tgagggagcg gttgacacag ttgtttgacg atctcatctc atcctcactt tttgaatcgc
       61 ttcgggtata ttccccgcaa gattgccgcg gggactttag atattgcgcg tgagttaaag
      121 agcggtcttg cttcggtgag cgtcgggcga cgcgtgcgtt ttcaactggc ggcgaatgat
      181 aagttccttc gcaacggtag tcaacgtttt tcgaaaacaa tgccggccgc gcaccggatg
      241 accgtttcat ccaataacgc gggagacggc gtcgaaaaat gacacggccg ggaaaagttt
      301 tttattgcct ctttcactcg ataacgaagg tttcttcacc ccgcacgccg gccgaacgcc
      361 gccccgctcg tcccttcaga gaaggagacg ccgaaccgcc tgcccccgca cacaagtttt
      421 ttagcaagtt gagtgttgaa aaaaaacgcg cgcgcgcgta gattgtcggc tgttcgtata
      481 aactttttcg aggcggggcc gcggcggggc ggggcagggc gttagcccgc gccggccgag
      541 gcaatgtaag accactggag gagcgtgcgt agtgccgacc ggaacttgcc cagagtgtga
      601 tgctgacgta cacgtggaca tcgactccga taagggagac gccgtcacgt gtgacgagtg
      661 cgggacgcat ttggaggtcg tcggactaga ccccgtcgaa ctcgacatag cggaggacga
      721 tgacgacagc gggtacgacg aagacgacga tgaggacgag tactgaaaac agtgataagt
      781 gataagtgat aagtgataag tgctcttagc tgttagctaa cagcaaggct tccttcttat
      841 cacttatcac tcatcactta tcactactct tgtacccgcc gcggctctgg cggacgctga
      901 ggccgcgcgg ggcttcgacg cgaacggtgt gatggcggcc gtcgcgtcgt ttttcttcgg
      961 gcgggtagaa ggccagcacg taggtcgaag tgacttcctc ggccaactcc ctgaagagcg
     1021 ggccgtagtc ctcgcggttg gcgtagacgc ttgcaccccc cgtgcgctcg gcgaggccgc
     1081 tcacgtcgag cggcgtgggc agcgggccgc ccaccgaggc catcgcgcgc gagtaggagg
     1141 ggagcgtcac ggagtagacg ctgacgccgg cggcgttggc gcgctcgatg acggtctcgg
     1201 ggcggacggt gtcgccgacg gggaagccgt cggtgatgac gacgacggcg cgcttgacga
     1261 cctgacggtc gcgcgtgcgc ggcgcgttgc ggacgagcag ccgcacggcg tcgtccacgg
     1321 cgtcgtatgt gtgcgtggag agtccgttca tctcgcgcgc cagacgcgtg aaggagcgcg
     1381 tgagcttggc ccggtcggcg gtgaaattct ggtagacctt ggcgctcatc ccgaagccca
     1441 tcaacgcgaa cgaggcggga cgcgccgaca ttccccgcac gaactcgtcg agcgccatgc
     1501 ggacgcgctc catctccccg gccgtcatgc tccccgacac gtcgatcagg aagacgaccg
     1561 cgacggggcg ttccgcgccg ccgcgccgct cgacgttgaa gaaagcaacg ggccgctcga
     1621 cgccgtcctc gtagattttg aagtcttccg ggcgcaggcc gcgcacgggc cgcccttccg
     1681 cgtcggagac gagcacgtct acgaggacga ggtcggtgtc gatacgcagc acctcgtccg
     1741 cgtcgatctc ctgaccggcg ggcggcggca tgcctgcgtc gcgcgcgggc ggcttctgct
     1801 gcgcgttcgg cataagcggc agcagcaggg cgagtaagaa cagacacggc gcgagtgcaa
     1861 ctcgggagag gtttttcttt ggaggggaag acgacataaa actccgggac gcgcgtgaaa
     1921 accacggaga gatgcggagc attatacgga cacgcgcaac gatgcgccaa cgtccctgtg
     1981 ggcgaggctt ggcaacacgt tcgcccggca cggcatcaac actgctccgg gccgagagct
     2041 tcacggccca atccgccgga tgtataatgc cggccaaccg cacggggagc tttcgccgat
     2101 tcccggctaa gggttgcgag ttcggagcgt gaacatcaaa agcataacga gacttttctt
     2161 atcactgctg ctgctgtcgg cggcgacggc cgtcgccgcg cccgctcagc agcaggacaa
     2221 gacgacgggc ggcgtcaagg gcaaggtgcg cgtcgagacc gggtcgtcag cgggggtgac
     2281 ggtcgtcgtc cgccgcggtg acgacgaggt caggagcgcc acgacggacg ggcgcggcga
     2341 gtttgaagtg cgcggcctca agcccggctc ctacggcctg acgttccgca agacggggct
     2401 gaaggtcggg aaggtggagg gcgtcgaagt gcgcgccggc aaagtgcgct cgctcggcgg
     2461 cggtctctac atgtcgatgg acgagggcgc gctggccatc attcagggga gcgtcttcaa
     2521 tcaagatggg aaaagcttga ccggcgcgaa ggtcgagctt gccttgttgt cgcccgacgg
     2581 cgccgcgaag aagttggaga gccgcctgtc gtccatcgag accgggcggt ttgtgttcaa
     2641 gctgacgccc gaggcggcgc gctaccgcct gacggcgacg gcccccggcc gccagccggc
     2701 gacgcaggag gtgacggtag acggcgcggc catttaccgc gtcgctttgt cactcgcgcc
     2761 ggcggcgaag tagcgagcgt gtgagtccca cttgccgtga aagacgaagc ccacaaagac
     2821 ggggagacgc gaaccgaatt tctgacggcg cgccagctcg gcgccgtcct tcaggtgagc
     2881 gaatcgaccg tgcgccgcct cgcgcactcg gggcgcatcc cctcgatccg catcacgccc
     2941 cacatcatcc gcttccgcct cgcggccgtc gtcgaagccc tcgacggcgc ccgtcgctcc
     3001 cgccgctcca ctcgcgctga agacaacggc gcggacgact cacaactgtc tttcttcgag
     3061 tagcacaaac ccgcctgagc acatccgggg cgggaaaagg gaacacggat gagacggatg
     3121 agcacggatc aaatccgtct tcatccgttc tcatccgtgt tctattaact acttgcggcg
     3181 gcgcgaaccc tgaagggcac tgaggttgat ccagcgcccg tcgcgcttga ggtagacgcg
     3241 cggctcggtc gccgggtatt gccgcgcaaa cgccgggttg gcggcgagca gcgggcgcac
     3301 ggtcgagccg tcgcccgtcg tgacgcgcca gcgcccgccc tcgcgcacca tatactgccg
     3361 cgagacgatc ttaccctcgg cctccatggg tttgcgtacc gtcgcgtaga cgaccgtgtc
     3421 catcaccgcc agattgccgg cgacgtgcgc gcgctcgatc tccatcccgt tcaactcgaa
     3481 gtgcccacgc gctttcccca gcgcctcgac gaaacgtgcg cgcgtgacgc gccgctgcga
     3541 ggccgaaggc aacgcgtcgt agacgccgcc gtagtcgccc gcccgcagaa gctcgaaggc
     3601 ttgctgaagc gccgcccgcg cctcgccctc gacgccgccc tgcgccgcgc cgcgcgcctg
     3661 agccgccgac gcgccgacca actggatcgt caacacggcg agcgcgacgc gccccgccct
     3721 cttaataacc gccagcctct tcctctgtat catcgtctgc atcctcgtcc tcaacccttg
     3781 cgcgctcctc gtcccgcccg acgtttgcgc ggtgcaactg ggcgcgcacg ttcagaaacc
     3841 acgcgacgac gccgagcacc gccgcggcga aagcccaatc ctgacgcccg agcaaaaaga
     3901 gagcgccgcc cgcgaccaga cacagcatcg ccgcgacgct ccacctctgc cgcgccgtca
     3961 cgtcagcgcc ggccttctct gtaaatccct ccatcgaccc tcaagcgcca gccgtcattc
     4021 gacgaccacg gggacggcgg cgttgatctt gcgcggcggg aaacactcgc ggtcgtcgca
     4081 cggctgaacg gtgaggtcgg cgctcaccgt gtgtctcccc ttcgacgcgc cggcatcggc
     4141 cgaaagcggc agcctgatgg cgacctcgcc ctcgtagacc gagagcggct gctcgctgaa
     4201 ggggaacttc tttttcacgc cttcggggta gacggccgtg cccgcattaa tcccggcggc
     4261 cggcgtcgct tcgagccgcg tcgggatgac gaacttgtca cccggcgcgt tgctgtttac
     4321 gtggaaaccg tccgcgatgt tcagacgcac gaccgcctcg cccgagccgc cggccgccag
     4381 cttcacctcc gacacgctcg cgcgcaccgc gtcggcgccc ggagccgacg cctgctccgc
     4441 cgtccgagcc gcgttcgccg gggtcgcatt ccggttgccc tcgcccgccc cgccgcacga
     4501 cgccagaaac gtcatcgcac aaaaaatgaa aagaaaagtc gctctgtgaa acatgtttcg
     4561 gagtctagct cagattgcag acgagcgcac cacggaggga aggcaaaagt aaaaaggtaa
     4621 aatgcaaagg tgagaagcgg gagcatgggc tacaaggagc cgcggagcgg cgaaagaatg
     4681 tagccacgtg cgccagcccg tggtaacagc aacaaaaaat gagaagcccc ggaggggcga
     4741 aagaacctcg gcatgcgtaa tacgccagca ttaggattct atcgcccctc cggggcttac
     4801 ttaatgttgt cccgcgtgtc cccacggctc acgccgtggg ttacattcta tcgctgctcc
     4861 gcggctcgct gtcgcccgaa gctcggcctc ttaactattg ccttttggct tccatctcat
     4921 ccaaccggcg gcgctgcggc agtcggcggc ggcggcgcgt tccggcggcg gcgacgcatt
     4981 gagaaacgga acagcagcat gagcggcagg acgatgagca ggaagacggg gatgagcgcg
     5041 atgatgacct ggatgagacc gagcgtgatg gagagcgcca cgtcgatgcc gttggcgaag
     5101 gctcgcttca ggccggggaa gaagccgcgc gtgctcgccc cggccagcac gcccgcgggg
     5161 cggagcgtca ccttgatggt cgaaagactc gactggttct ccagaaagcg tcggcgccct
     5221 tcgagccgct cgatctccgt ccgcacggtc gccagcgcgc gctgcacttc gagcgcttcc
     5281 gtcaccccgc cggcgcgctt cagcagttcg agcagttgcg cttcgaacgc cctctgcgtg
     5341 cgcgcgcgcg cctcgaggtc gatgtattcc tcggtcacgt cctgccccgt gatcttctcg
     5401 tcaacgaccg cgctgccgac gccgcgaatc tcgccgagca cggcgtcgaa ctgcgcgaac
     5461 gggatacgca cttccatcgt gacgacctcg tagccgcgct caccgtcgcc gtcctgatgc
     5521 ctcgactcgg aggtgacgac gaagccgccg cgctgctcgg caatcgaggc gatgcggcgc
     5581 tggccctcgg cgggcgcgtc gctctcgatg gtgagcgccg cgttgcggat gatcttccgc
     5641 tccgtcggca cgctcgccgc ctgcgacgcg tccgcctgac taagcgacac gtctctgatt
     5701 tccgcctccg ccgtcgcgcc cgcgctcgac cgctcggctt tgccgccgct ggtcgtcaag
     5761 gcgccggaat caggtgcggc cggccgagag gccgaattgg ccccgccctc actgcggctg
     5821 caacccgcaa gggcgagcag gatcagcacc gacgacaatg cagtcttcaa acgcatgtgt
     5881 aacctccgcg gcttccgaaa atccgttcgc cacaggaacg ccccggcgcg ctcccgcttg
     5941 cgtcctcgtt cgccgacgcg atcatgtctc gggacgtggg gcgcgcgcca tgtgggcggc
     6001 gcggtctggt gggtgtggcc gtcacctgcg cgcggactct gcgcgcgcgc ggccgccgaa
     6061 gaggccgcgc gcggaggcga gcatcttccg caggcgtcgc agaaccttcg gcagtagcca
     6121 cacgagcagc aggaggaaga cgaagatgac ggcgagaatc acaagcgggt ggaggacggc
     6181 gagcacaatc gccccgacgg cgaccacgtc ctcggcgagc gagagcgccc agttcgtcac
     6241 cggctccggc gaggtgttgg cggcggcgcg cacggtcgcc ttcgtcccgt gggtcgagag
     6301 agcgagcgtg ccgcccaaaa ggagcgcgac gacctgcacg gccggcgcga gttcggtcgt
     6361 cgcagcgagc gccagcgccg cgcccgccgg cacgcggatg aaggtgtgga caacatccca
     6421 caccgaatcg acgtagggca ccttatcggc gacgaactcg acgacgtaga ggccgagcgc
     6481 cacgccgatg atccaccagt tgtcgagcac ttccagcccg cccggcagtt gcgcgaactt
     6541 gtagtgctga agcaggccga gcacggccac cgtggcgtac aggttgatgc ccgatgtcca
     6601 cgccgcgccg agcgccgtgc cgagaatggc gatgacgttc atcgaattga aatgaggaac
     6661 aacgctatga ccttccgttc cgaccttcgc tgaaatagcg gtcaacgaca gccgtcaggt
     6721 ttttcaaatt ctcctcagtc ctcatctggg catcgaccag cgcgttgatc ttcgcgttaa
     6781 catctctgaa gccttcaagc gtggccgccg ccagccggtt cacgatctgc tcagtctgtg
     6841 cctgagtctc ttgcagcctt tgctgtccta ccgccagagt cgcgaggtgt tctgcgacca
     6901 cgtcaaactt cttgttgatt tcctcgtcgg tcatttcttg ccacctttca catcgtcaac
     6961 tgacgcccgc cgcatcattc agcactttcc tcctcttcgt cgccgccgtg tttgtccgcg
     7021 cgcttgatgc cgaacttctc caggcggtat tggagggtgc ggaaggtgag gccgaggagc
     7081 ttggcggcct tagtgatgtt gtagtcggtg cgctccatcg cctgaaggat gagctggcgc
     7141 tccatgtctt caaagactaa gccctcggcc gggagtttga agccgccctc gggcgagggc
     7201 cgcgcctcct gccggacttc gagcggcaag tcctcaatcg tgatgaggtc gctctcggcc
     7261 aaaagaatcg cgcgctcgat ggccgactcc aactggcgga cgttgcccgg ccacgagtag
     7321 tcgttcatca ggaggcgcgc gtccggggtg atgccgcgga cgagacgcga ggtgttgcgc
     7381 gtgtgcttct tgatgaagta gtcgatgagg agatcgatgt cctcgcgtcg ctcgcgaagc
     7441 ggcggcacgt cgatggagag gacgttgatg cggtagtaga gatcgtcgcg gaagcgcccg
     7501 tcggagacca tcgcgcgcag gtcgcggttg gtggcggcga cgacccgcac gtcgatcttg
     7561 atggcccgcg tgccgccgac gcggcgaatc tcgcgctcct gcaaggcgcg caaaagtttc
     7621 gcctgcatgg acacgtcgag ttcgccgatc tcgtctaaaa agagcgtgcc cttatcggcc
     7681 acttcgaaga ggcccttctt ctcggcgtgc gcgccggtga acgacccgcg ctcgtgtccg
     7741 aacagctccg actccaaaag gttttcgttg atggccgcgc agttgacggc ctgaaacatc
     7801 tggtcggcgc gcaggctctg cgtgtgaatg gcgcgcgaga tcagctcctt gcccgtgccg
     7861 gactcaccgc ggatgagcac ggtcgagttt gagcgcgcga cctttaagat cattttcttg
     7921 acggcctcca tctcgcccga gaccgacatg atctcggtgt cgagggcgtc gagacgggcg
     7981 agcgcgcgct ggatggtttc gagcagggtc tcgcggtcgt agggcttctc cagatagtcg
     8041 aaggcgccgc gccgcagcgc ctccttggcc gaatcgaccg agccgtgcgc ggtaagcagg
     8101 atgacgatga tcgaagagtc gtaggcgagg agcttggaga gcagctcgat gccgtccatg
     8161 ccggtcatct tgaggtcggt gagcgcgaga tcgaaccgcc gctcacgcgc gatgcgcagc
     8221 gccgcctcgc ccgagctggc cgtcgtcacg tcgtatccgg cctccgagag aatcatttcc
     8281 agaatctccc gctgcgactt ctcgtcgtca accacgagta tgctcttgcg tgccatagtc
     8341 aatcagtggt tagaggttag aggtcagagg ttagtgaaag accgaaatgc tttctactga
     8401 cctctgacct ccgacctctg acctctttct cctatcggta aagtgaccgt aaatgtcgtg
     8461 ccctcgtcct gcttgctctc gacggtgatg tggccgccgt ggtcgtcaac ggccttcttg
     8521 acgatggcga ggccgaggcc ggtgcccgtc tccttggtcg agaagtaagg ctcgaaaatc
     8581 ttggggaggt tgtcgggggg gatgccgcgg ccggtgtcgg acacgccgac gcgcaccacc
     8641 ccgtcgtccg atgagagcct gatcgtcagg cttccccctt cgctccccat cgcctgcatg
     8701 ccgttaatga taaggtttgt gaagacggaa cgtaaagact cccggtcgcc gagcacttcg
     8761 agcgcgcccc cctcgcgctc gacggcggta cgcacgcccg tctcctcggc ctgcacctcc
     8821 accatgcgga gggcgtcgtc gaggagcgcg ccgaggtcga tgggcttgag gtcgagggcg
     8881 gccgggcgcg agtatttgag gaactcggtg atgcgcgtgt tgatgcgggc gacctcggcc
     8941 ttgagttggt tggctaagcg gccgaagagt tcgcgcttct gcgggtcttc gggggcgaga
     9001 ctcgtgcgca ggtgatcgag cgtgagattg atgtagttga gcgggttgcg cacctcatgg
     9061 gcgatggccg aggcgagacg gccgacgacg gccgactgct cggcctgttt gactttcacc
     9121 tccagctcgc gcgtgtgcga tagacccgcg atcatctcgt tgaaggtcga gacgaggacg
     9181 ccggtctcgt cgcggcggtg gccgccgggg acgcggaagt cgaagtggcc ggaggcgacg
     9241 cgctgcgtgg cgcgcgacag cgccttgatg ggacgcgtga agcgccagac gaggatggcg
     9301 gcggccgtcg tcgccagcag gaggacagcg agggtgggca ggagcgggcg caggatgtcc
     9361 tcccatgagt cgttctgcgg cgtcttcgtc gccccgagca cgacgatgat gtagttcacg
     9421 gtcgggcccc cctcctcgtc gatgagtctg acctgcatcg tgaaggcgcg cggctcgccg
     9481 gcgatggggg tcgtcgtggc ggcgctgccc tccggggcgt tgatgagccg catcaagtct
     9541 tccgtcacgg ggccggcgac gacgaggcgc ggcaggttga gttcggagac gtgataatag
     9601 cgggcctcgc cgttgacggt gcgcggggcg aactgcgggt cgaggctgtc gtcaacccgc
     9661 ccctgctcgt tgatgacgag gacgttctgg acacgccccg cctgctcctg gaggaaggag
     9721 acggggcgcg tctcgtccat ctcccacaga tattgtctcg tcggggtggt gatgctttcg
     9781 agcgccagcg ccgtgctcgc ggccagcgcc tgctcctgct cggagatgat ggcggcgcgc
     9841 cgcatctctt cgcggtagaa gaggtaatac tggacgccga gcgtggcgaa aagcaggagc
     9901 gccagcacca gcagcaggcg cattcggaag ctgtagaaga ggttgggcag tttcatccgc
     9961 ggtctttttg tcccactttc cccggcgtgt cggcacgtcg cgagcgccgg cgcgggggga
    10021 gcgcgcggcg gggcgacgat attttttatt tgccgtttcc gccacccgga tgttatattc
    10081 cgagctgtct ccagcgcaaa cccctcccgg agacagtttc tctccagccc tgtcggcgac
    10141 ggattctaaa gtcggagcag tatcgagtga tccaaggcga aagagctttg tcgtcaacgt
    10201 ctttcaaaac ttcgaccccg gcgcgccccc gtggcgacgc cgtccgtcct tgcacgcaaa
    10261 cgaaaacctg ttcagcgcat cataagcgtt aacccggcgg catcgaccgg ttcgcccctc
    10321 cctgaagaac cgcgtcgagg ccgtcaagtt tccattcgcc caggagtgta gacagtccta
    10381 tgactcgtag agtgtccccc gcttccgcgc tcgccatcct tttcatcttc atcagtctcg
    10441 ccgtcgtccc cgtcgccgcc tccgcgaaaa agacgccgcc gaagaagacg cccgccccga
    10501 aggccgcggc ccggaagtcg gcccgcgacg accgtcgggg gcgcgagaag gcttcgtcga
    10561 agagggaagt cgcccaaaag aactcgcgcc agagggacgc ccgcgcctcg aagaacgagc
    10621 gcgccggccg gggccgcgac cgcgcctcga agaacgaccg cgcgtcgaag aatgagcgtg
    10681 cctcgaagaa tgagcgcgcg tcgaagaatg accgccgctt gagtcgcgcc gagcgacgcg
    10741 agcaggcgcg cgaaagtcgc aaggactcca agcggatgtc gcgccgcgag cgcgaggccg
    10801 ccgcgcgcga gcaggcacgc cgccgccgtg aggccgaaga gcgcgcccgt caggccgcca
    10861 tcgcccgcgc catctatctc gcacgcatcc gcgcgcagga tcaggcgtgg cgcgacgaga
    10921 cgcaggccaa catccggcgc gacgagacga cgggcgaaga cccggaggtg cgccgcgcgg
    10981 ccatcgaggc gctcggcaac aacgccggct cggtcgtcgt catggagccg aagtcggggc
    11041 gcgtcctcgc cgtggtcaat caggactggg gcctgcgccg cggcttcaag ccttgctcga
    11101 ccgtcaagct cgtgacgggc ctcgccggac tttccgataa cgtcatcgac cccgtccaga
    11161 ccgtcaacac ctccgccggc acgctcagca tcgacctcac cgactcgctc gcctactcga
    11221 acaatcatta cttccagcgc gtcggcggtc aggtcggctt cgaccgcatg atgcactacg
    11281 cgcgtgagct gggcctcggc cagccgaccg gcatcaacca ccccggcgag tcggcgggcc
    11341 gcctgccgct catcaaggag ggcttctcgg tgaaccgcat gtcgtcgcac ggcgacgaca
    11401 tcgaggtcac gcccatccag ctcgccaaca tggcctcggc catcgccaac ggcggcgagc
    11461 gcctcgtccc gcacctgccg cgcacgcccg ccgagaccac gcgcttcgag cgcgtcgtgc
    11521 gctccaaagt taacgtcccg caggagcacc tgcgccggat gctccccggc atgatcggcg
    11581 ccgtcaacta cggctcgggc cgcacggcct acgaccccgc gcagaccatc gccggcaaga
    11641 ccggcacctg catcagtcag gacgccgccc gcacctggct cggtctcttc acctcgttcg
    11701 cgcccgtcca cgacccgcgt ctcgccgtgg ccgtcgtcac gcgcggctcc agccagcgcg
    11761 gcaagatcgc cgccggcatc gccgggcgcg tctaccgcag cctcagccac cgctacggcc
    11821 cgcgcgccgg cgaccggccg atgttggccg aggagacgct catcccgcgt ccgaaggttg
    11881 atccgagcaa ggccgccgtc ttaaacgatg aagaggccga agccgaagcc gagtcgaacg
    11941 ccgtgaactc ggacgcctac gtcgtcaagg aggccggcaa cgtcggcgtg cgacaggtgt
    12001 tcgagggaca gcagaacaac gtcaagcgga cggcgaagat ttacgagaag ccggccgtga
    12061 cttccgcgcc cgtgaccgtc gcccccgcgc ccgcgcagac caacgcgccc gccggcgcca
    12121 cccgcccgcg ccgcgtcggc gccgagggac agccgcagca gcagcagtag taatcgccga
    12181 cgcggctccc tccgtcaggc tccgcgctcc caggcccgtc gcccccgaga aatcagggtg
    12241 acgcgcctgg gagtattatt tttgtgtgaa caattgatgt agctttggcg gtgggcggcc
    12301 cgccgtttcc ataaggcttg acactctccg acaggttgta gcagtatcac gaacgaaatc
    12361 tcctgaacga ccgaaaatcc cctgagccgg cggctctcgt atctccctgg tggcgcggcc
    12421 tggagcgccg cccgacacga aagcggcaac tgcccgcaaa gccggcgcat cttaaaagtt
    12481 aagcgacgca ggcgactgaa agccctcgcg cccccgtcag aaaatttgca gcgacatcgc
    12541 ttggaggaca ttagaccgat gaagaacttt ttacggttca tgacgctaaa cgcgattgtg
    12601 gcggcctgcg ccctctcggc gtcggcgcag gtgcagccgg cgcaacccaa cgcgacgccc
    12661 gacgccgacg cggcggccaa ggcggcctgt actgacctct atacgaagtg gcgcgacaac
    12721 tacaagggcg acgccgcgaa gcagaagatc gcctacgagg tgggcaaaca gttcgtcact
    12781 cagtgcccga gcgacgagta cctctcctac gtccagaagt ggattccgaa gtacgaggcc
    12841 gccatgcagg gcgatcaact gcaaaagcag tacgacgact gcgtgaaggc cagcgattgg
    12901 aagtgcattc tcaactccgg caagcaactc gcggcgaaag accccgagaa cctgacgctc
    12961 tatctcaacc tgacggccgc cggttcttcg agtaaggaca agacgctctt cgcggagtcg
    13021 ttgaacgccg cgggcagaac cattcaactc gtcgaggcgg gaaaggccga cgccttcacg
    13081 ccggagatgt ggaagggcat tcccttcgcc agcaacaagg ccgagctgct cggcttcctc
    13141 aacaacctgt cggctttcta cagcctcgac acgaagccgg ccgaggcggc caagtacgcc
    13201 ttgaaggccg tgcagagtga gggccgttac aagaaagacc cggccggcta ttactacctc
    13261 tcactggcct atcaggccgc ggagtacgcg ccgcaggcgc aggcctacaa ggccgcctgc
    13321 gagggtaagg agctgaacga cgagtgcaag atgaagctcg acacgctgta cctcgtcgtt
    13381 gaccgcatca tcgacgcgca cgcgcgcctc gtctccctga ccacggacgc gacggcgaag
    13441 gccgcgcgcc tcaaggagcg tgagggcttc tacaggttcc gaaacgagga caagatcgac
    13501 ggcatgaacg agctggtcgc cggcattcag gcgaagccgc tgctcctgcc gagcatgcag
    13561 acgatgccga cgccggcccc ggcgccttcg accacgacga cgacgccgtc tgccaccacc
    13621 agcaaccccg gcacggccac gacgacgccg gccgccacga ccacgacgac gccggcgccg
    13681 gccaacgccg ccaagccggc gacgacgagc accacgacgc cgacgcccaa gcccactaac
    13741 ggcacggccg ccaagccgaa gccctaagta gggccgcgcc gggtcttcgg ttcccgacgc
    13801 gctgccaact ctccaagcag cctgactcgc aagcgcgggt caggcagtcg ctaacgcgcc
    13861 gggggcgccc atccgagttt ttcggacggc gtccccggcg cgtttttgtt acaatcccgc
    13921 cccagcatga ccgacgacac gcccaccgac accgcaaccg acttcgacgc gctcgaacag
    13981 gcgctcgatt acgacttcga gaaccgcgcg ctgctggagc gcgccctcac gcaccgctcc
    14041 tgggcgcacg agcacgtggg gccgggcgag gaggcgcgcg cccgtcagtt gcacaacgag
    14101 gcgatggagt tcgtcggcga ctccgtgctc ggcctcgtca tcgccgacgc gctcttccac
    14161 tcgcaccccg aagtgaccga gggcgaactc tcccgcatga agcaccgtct ggtgagcacg
    14221 cagacgctcg cccgcgtcgc gcgccggatg gggatgggcg agcacctgag agtggggcgc
    14281 ggcgaagaga agaccggcgg ccggcgcaag cgcgcgctcc ttgccgacgt tttcgaggcg
    14341 gtgctcgcgg ccgtctatct cgaccgcggg ctggaggcgg cgcgcgcctt cgtcctccgt
    14401 gcgctcgaag aggagatcga gcaggccagc cccgagcggg ccgccgccct cgacaccaag
    14461 acgttgcttc aggagcgact tcaggccgag cgccacgtcg cacccgtcta caacctcatc
    14521 gagaccgagg ggccgccgca ccatcgcgtc ttccacgtcg aagtctgctg ggacgaacgg
    14581 gccgtgcgcg gcgagggcgg caccatcaag gcggccgagg ccgaggccgc ccgccgcgcc
    14641 atcgagcaga tgggcgccgc ccccaccggc gaagccgccg cgcccgaagc cgttcgagac
    14701 gaagccgcgg actgaagcta cgcgcgccgc cgcgctgccc cgcgtcactg cgtcacttga
    14761 ccgcccccga tttcgttctt acattcatct gtaatggaca acgagcagac gggaacgggc
    14821 gtcgccgagc cgccgccggc cagtgcgggc gacccttacg caaactctgc gggcgcatcg
    14881 gaagagagaa ggccgctcgc gagcgcgccg gaagagacgc ggccgccgca ggggccgccg
    14941 cgcggggttc tgcgcgaata cttcgagtcg ctcgtcgtga cggcgatcat ggcgctcttc
    15001 gggatgacgt tcgtcgtgca ggcggtcaag gtgcccacgg ggtcgatgca gaacacgatc
    15061 ctcatcggcg accacctcct cgtcaacaag ttcatcttcg cgccggggcc gcccgtcttc
    15121 ttcctcccgc aacgcgaaat ccgacgcggc gacatcgtcg tcttcaagta ccccggcaag
    15181 tcgaaagacc cgcagttcga gcgccacttc atcgacgaca cgcgccccga caacattccc
    15241 ttcaagatca actacgtcaa aagggtcgtc ggcctgcccg gcgacgtgct ggaacttcgc
    15301 ggctcgcagg tgctcatcaa cggccagccg ctcgacgagc ggcgcgtccc cgccacgaac
    15361 ccgcccgacg acgacgacga gcgcacgagc ggcgacgaga gcggcgcccc gctcggcgtc
    15421 gccgccgaca tcccagcggt cggcgaaggc ccctacgaag tttactggag gcccgacagg
    15481 ttccgcgcgt cgcgcgccgc cacgaccacg gcgggcgaga agttcgccgt gcccgaggag
    15541 cactacttcg tgatgggcga caatcgcgag aacagtcagg acagccgcta ctggggctac
    15601 gtccaccgcg acctcgtcat cggccgcgcg atgttcgtct actggtcttt cgacgagagc
    15661 ctgccccgca gcgaagcccc tttcccctta aacgccctca gtgacttcgt ctccaactcg
    15721 cgctggggcc gcacgggcac gctcgtcaaa taaacaaaaa gaatcgtctc gtgacgcttt
    15781 ctccttcccc cggagtaaga atgtctcgcc gtaaggaggg gagaaaatga ttaaagcact
    15841 cagcctcggt ggcctgagct gggccgagtt gggcaagcgc gtctggagag agatagagga
    15901 ggacgaagtc ttcggacgcg ccgctcagct ctcttattat ttcctgctcg cgctcttccc
    15961 gctcctgctc tttctcacct cgatcctcgg cctcatcgcc gacgagaacg gcgagctgcg
    16021 taagagcctc ttcgagtacc tggggtcggt gatgccgggt gaggcgtcga agctcgtcag
    16081 cgacgtgatc gatggcagcg gcggcggcaa gctctcgttc ggcattctgg cgacgctctg
    16141 ggccgcctcc aacggcatgg gcgctatcct cgaagccctc aacgtcgcct acgaggtgaa
    16201 ggagacgcgg ccgtggtgga aggcgcgcat cgtcgccatc ggtttgacgt tcgcgttggc
    16261 gctgctcatc ctctcggcgc tcgtcctcgt gctctacggc cataacatcg ccgagaccgt
    16321 ggcgggcagc ttcggtctgg gccgcgcctt cgagcttggc tggaaggtct tgcagtggcc
    16381 gattgtgctg gccttcgtgc tcctcgcctt cgcgctcctc tactacttcg cccccgacct
    16441 ccacgatcag gactggaagt gggtctcgcc cggcgccgtc ctcggcgtcg cgctctggct
    16501 catcgtctcc ttcgccttcc gcacctacct gcaatacttc aacacttaca gcgccaccta
    16561 cggctcgctc ggcgctgtca tcatcatgat gctctggttc tacttcacgg gcctcgccat
    16621 cctcgtcggc ggcgagatca attccgagat cgagaacgag atggccaagc agggagcgcc
    16681 cgacgcgaag gagaagggcg agaaggcgcc ggacgacggc aagcccgagg ccgccgcgtc
    16741 ggcagagggg acgagaggga aggcgtcagc cgatgcgacg ggagggacgg ccgtcgctag
    16801 gaaaaccgac gcggtcgcag tgaagaagcc acagaagcag acggagaagg cgggaaggtc
    16861 tgcacaaagt tctgtacctg cccgccgggg ccgcgactcc ggcgcgggcc tgacaagcaa
    16921 cgcctctcag ggacgctcgc gcctcaattc tgaagagatg agaaccgcga aaagaaccgc
    16981 gcgctccttc agcctcggca aggcggtcgt cgtcctcggt gcgtgggtcg tctcgaaagt
    17041 gcgcggcaag cgttaggcgg ggtcggcgct tagcgtgtgc tcaactgcgc cgccgccact
    17101 tccatcacga ggagcttctt gatgccgcga tagagcggct cgatttcgca ccactcggtg
    17161 agggcgtgcg agtcgccgca ggggccggcg ccggtcgaga tcgaggggat gccggcgagg
    17221 agcgcggcga tggaattgtt cgaggccgtc atcgtgatgg cgccctcaaa gcccaactcg
    17281 cggtgaacgg cctccatcag cttgacgaaa ggcgactcgc gcaggcccgg tatctgcgcc
    17341 gccggcgtct tcgagatgac ttcggtcttc accgtcatct tctcgcgctc ggcctcctcc
    17401 cgcaccacgt cggcaatctt cttctccaac tccccgatca cttcgttact cgtcgagcgc
    17461 agatcgacgg tgaaccacgc gtcggccgcc ttggcgttga cgacctcggc gccgccgagc
    17521 atgccgatgt tgacgtgcgt cggcgggttc tcgggcaatt cgagttcgta gatgcggcgg
    17581 acggcgcgcg ccaaggggag cgcggccgag tagggcgggt tgcgcgagcg cgtgtgaccg
    17641 ccggggccga tgaggtggtg cctgtaccag ttgatgccga tgccgccgta ggtgaagccc
    17701 tcgtagccgc cgtcgagcgc gatgtaaaag tcgatgcgcc ccttgttgtc tttgacgtac
    17761 tcctcggcgc cgcgaagcgt ggtctcctcc tcgaccgtga agacgaagac gaggtcgcct
    17821 ttcgtcttca cgttggcggc gtcgagcgcg cgaatggtcg ccagcatcgc ctcgatgttg
    17881 cgcgtatcgt cgccgacgcc gggggcgtag acgcgcccgc cgcgcacctc ggccttgatc
    17941 ttcaagccct cctgaaagac cgtgtcgagg tgcgcgtcga tgacgaccaa ggggccgccg
    18001 cccgtcccct tgcgcgtggc gacgaggttg cccgccgagt cggagcgcac ttcgagcgac
    18061 gcggaggcgc gcaggagctt ctcgacgtag agcgcgcgct cgcgctcctt gcccgacggc
    18121 gcgttgatct ccgtgatcgc cacccactcg cgcaacgtct ccgcgcgctc gcgatctacg
    18181 tgggcgaagg ccgctttgac ggcgggacgg tccaacaagt ctttggcctg ttcgattgtc
    18241 a
//
LOCUS       DASVES010000170         9458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1685126, whole genome shotgun sequence.
ACCESSION   DASVES010000170 DASVES010000000
VERSION     DASVES010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685126"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1010)
                     /locus_tag="VF240_06880"
     CDS             complement(126..1010)
                     /locus_tag="VF240_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7174988.1"
                     /translation="MKTPLPVVDGVGPSCQWLPAGPWKTVLDFLKERYPGVEVATWLA
                     RMRKGQVVDETGLRLDRGSPYRVGACIFYYREVESETIVPFDEHVLHRDEHILLADKP
                     HFLPVVPAGRFLHETLLVRLKKKWKLEWLVPVHRLDRETAGVVMFSLNPATRGAYVSL
                     FRERKVKKMYEALARIRPGLSLPLTRRSRVVAGEPFFRMREVEGEPNSETHIDVLRRL
                     NGVGLYQLRPVTGRKHQLRLHLSALGIPIVNDKLYPEMCDAEDDDFSNPLKLLAKSIY
                     FLDPLTGRERYFESGREL"
     gene            complement(1021..2607)
                     /locus_tag="VF240_06885"
     CDS             complement(1021..2607)
                     /locus_tag="VF240_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7174989.1"
                     /translation="MKRTVAFCLSALLCVSPLCAQAQQPAASAAEVENVELRRRVREL
                     EERLQRAEALLKPGRDAGALDAGATSVQRAREPEDEPAASSGGPRQEQPQENELLRFF
                     KSTTVNGYIDTYYAYNFNRPHDALNSLRAFDTRHNQFSLAAVGLAFEKTPDAASRVGY
                     RLDLLFGPAADYLASAEPGGIEVYKHIHQAYGSYLAPVGSGLQLDFGKLISWSGAESD
                     QVLENWNYSRGLLYSFASPTYHMGLRATYSFGPKVTVMGAVVNGWNNVEDNNGGKTVA
                     LALTLNPTNKFSLTQNYIFGAEQPGDRRHKRHFFDTIITYDFNDRLAFLGNYDYGFDR
                     LADGSRVRWQGVAAALRYAPAGRFAISPRVEWYADYDGFTTGAAQRLREATLTGEYRV
                     GHGLTARLEYRRDWSDKHVFETAAPSILKRTRATLASGLIWSFDSAERKPPEPEQSPP
                     AAPPATTTSRATASVARIENPAPPPVPQNQDDAALRVIARPAPPPHRTRERRRGAVTD
                     APAVFTGSLYGFQSPTNRQD"
     gene            complement(2612..2767)
                     /locus_tag="VF240_06890"
     CDS             complement(2612..2767)
                     /locus_tag="VF240_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174990.1"
                     /translation="MDGQDEQDQKIGNLKSEISPFFILHILSIHVNYSSDKTGRGLAC
                     PYIHHPR"
     gene            complement(2885..4024)
                     /locus_tag="VF240_06895"
     CDS             complement(2885..4024)
                     /locus_tag="VF240_06895"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174991.1"
                     /translation="MIGPSYPAARAVAAMVQAHFARHLAALRQYGPEEVSQRPDAEII
                     EAIIDAAFWASLRREEGYSPKISLAFLPPGRAGQSLMFQQPLSLTPAALARLAPAVER
                     PGIHLGVWLNGEGLHVWGTTRTIPRFCFVLEVIEPGLLVVKYRRGQESGKFVNVVVLK
                     GDQIKEVDEAGTSLPDCPALVTSLLGFGSSASGEDSLNVLVQLAASMRLHGRGGSLLV
                     VPQDTEAWRESIVWPVPYSVAPPYSELAELIRQDPAERNKRLWQEALIDAVEAVAGLT
                     AVDGATVISDQYEILAFGAKIGRRKGYPQVEQVVLTEPVIDGVSSVVPPIQVGGTRHM
                     SAAQFVQDQHDSVALVASQDGRFTVFAWSPCEEMVHAHRVETLLL"
     gene            4278..4802
                     /locus_tag="VF240_06900"
     CDS             4278..4802
                     /locus_tag="VF240_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174992.1"
                     /translation="MVFLLAAVLLPTAWQYILMARRRGLLYVVKSIAAGAALGLLAGV
                     FIGVGARTGMSAITVANGDAPSLTLSGSFTVVYTFASFGIVLGVVYDGLFRRLLRRRG
                     LVYGVLLMLCSWYPLAQAAGQQLTGRPALITQIMVSGVIVALMWLPFGVALEALLSRW
                     HNRRAASAAASAAV"
     gene            complement(4827..7466)
                     /locus_tag="VF240_06905"
     CDS             complement(4827..7466)
                     /locus_tag="VF240_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7174993.1"
                     /translation="MEWFNVISARLRALFGREAVISDIEEELRLHVEMETEANIGRGM
                     TPEEARLAARRSFGNPGRMAERAYEVRGGGIVETFWQDIRYGVRSLLKQPGFAAVAVL
                     TVALGVGANSTIFSFVNGVLLRPLPYREPGRLVLLDETAPKRGVNAMGVSFPNFEDWR
                     EQNRVFEDIAAYNAGSYTLVGDGEPEQMRGARVSSGLFEILGVAPTMGRTIRAEEDRP
                     DNDTVVILGHGLWQSRFGANPEIVGRTISLNNRQHTVIGVMPPDFRFPEVADLWIPMA
                     LDEQRWTRNDHGLDAVARLKPGVTLEQAQAEMAAIAARIEEQNPVTNEGLSVSVSGLH
                     KGLVGDYRQALLILLGVVGFVMLIACVNVANLLLARASARQKEISIRAALGAGRWRIV
                     RQLITESLVLGLAGGALGLVLAVWGLELLLAAIPIEFPFWMKFNLDWRVLTFTAGVSL
                     LTGVVFGIAPALQASKVDLNETLKEGGRSAAAGAGHGRVRSLLVVAEVALSLVLLIGA
                     GLMMRSFLRLQHVNPGLNPENVLTMTVSLPRAKYKEQEKRPAFFGQLLERTRALPGVE
                     AAGAASNLPLSGSLWGRSLTVEGRPVLSVGEAPMINYCVITPDYFRSMGIPIMTGRDI
                     SETDAKDALKVTVIDERLAREYWPEESAVGKRVRFGPPESNEPWFTVVGVAGEVRHER
                     LDRQTRKTVYVSYQQIPEPQMTLTARTSSDPAALATAVRARVKELDPDQPVTNVRTMK
                     EVVARSVWQPRLYAILFGVFAAVALVLASVGIYGVMSYAVSQRTHEIGIRMALGAQAR
                     DVLKMVLGQGLLLALIGIVIGLGAALALTRLMATLLYSVSASDPTTFAAIALLLMGVA
                     LLACYIPARRAMKVDPMVALRYE"
     gene            complement(7482..7814)
                     /locus_tag="VF240_06910"
     CDS             complement(7482..7814)
                     /locus_tag="VF240_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7174994.1"
                     /translation="MSQVKADLLQGTLDMLILRAVSLGPLHGYGIIQRIRQMSEEMLN
                     VEQGSLYPALYRIEQRDWLTSEWDTNETGRRAKFYSLTRAGRRQLKIEEESWDRLALA
                     IAKVRQAT"
     gene            complement(8040..8918)
                     /locus_tag="VF240_06915"
     CDS             complement(8040..8918)
                     /locus_tag="VF240_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7174995.1"
                     /translation="MDSDSVAALNDAGASGAGEHLLSRILLGDVESVRSRLADGLEQL
                     GYYVLKEEPLVARRAARGWASWIGSNNVLNYSVALTIAVKPLSAGVARITFCYALKHR
                     MLTNSGRQTLTREAEAMIALAAMRSASAGCVACGATAAVGSRFCRQCGTPTVSSEPAE
                     VEVLKVTAGLCAGYTRTVFGAIILALTFIALLLGMTTAALPRTNVVVGVSLLLGALGW
                     ALLLTGLRRLHYTLNPKGETGVVLPVGNPQTGALSDMEASHSMRVPSEKTTALLSGAR
                     DGQLPPAPKRDREDHV"
BASE COUNT         1701 a         3043 c         2977 g         1737 t
ORIGIN      
        1 ggtagcgagc gggttgcgtc ttgcgtaacc acacccgctc gctaccgctc gcggtactgt
       61 gtatccttgc ccttacgcct ctctgcggtg aacgcggctc gccggacact caagcgaaaa
      121 gtgctctaaa gttctctacc actttcaaaa tagcgctccc gccccgtcaa cgggtcgaga
      181 aagtaaatcg acttcgccaa cagtttgagg ggattggaaa aatcatcgtc ctccgcgtca
      241 cacatctcgg ggtagagttt gtcgttgaca atcggaatcc ccagcgccga gaggtgcagg
      301 cggagctggt gcttcctgcc ggtgacggga cgcagttgat atagacccac gccgttcaat
      361 ctcctgagca cgtcgatgtg agtctcggaa ttcggctccc cctcaacctc acgcatccga
      421 aaaaaaggct cgcccgccac cacccggctc cgccgtgtca gcggaagact caggcccgga
      481 cggatgcgcg ccagcgcctc gtacattttc ttaaccttcc gctcgcgaaa gagcgacacg
      541 taagcgcctc tggtcgccgg gttgagagag aacatgacga ccccggccgt ttctctgtcg
      601 agacgatgaa cggggacgag ccattcgagt ttccactttt tcttcaggcg gacgagaagc
      661 gtctcatgga gaaatcgccc ggcgggaacg acgggcagga agtgtggctt gtccgccaag
      721 aggatgtgtt cgtccctgtg aagaacgtgc tcgtcgaagg gaacgattgt ttccgactcg
      781 acctcgcggt agtaaaaaat gcaggccccg acgcggtaag ggcttccccg atcaaggcga
      841 agcccggttt catccaccac ctgccccttc cgcatccgag cgagccaggt cgccacttcc
      901 acaccggggt atcgctcctt cagaaaatcc agaacggttt tccacggccc cgccggaagc
      961 cactggcaac tcgggccgac cccatcgact accggaagcg gcgtcttcaa agcaacctca
     1021 tcaatcctgc ctgttcgtcg gcgattgaaa gccgtagagg ctccccgtga agacggcggg
     1081 agcatccgtc accgcgccgc gccgtctctc tcgtgtccgg tgcggcgggg gcgcgggacg
     1141 agcgataact ctcagggccg cgtcatcttg attctgagga acgggcggtg gggcgggatt
     1201 ctcaattcga gcgactgagg cagttgctct ggaagtggtc gtcgcgggcg gcgctgcggg
     1261 cggtgactgc tccggctccg gcggcttgcg ctcggctgag tcgaacgacc agatgagacc
     1321 gctcgccagc gtcgctcgcg tccgcttgag aattgacggg gccgcggtct cgaagacgtg
     1381 cttgtccgac cagtctctcc ggtattcgag tcgcgccgtc aggccgtggc cgaccctgta
     1441 ctcgcccgtg agggtcgcct cccgaagacg ctgcgcggcg ccggtcgtga agccgtcata
     1501 gtctgcgtac cactcgacgc gggggctgat ggcgaagcgc ccggccggcg cgtaacgcag
     1561 ggcggcggcc acgccctgcc agcgcacacg cgagccgtcg gccagcctat cgaagccgta
     1621 gtcgtagttg ccgaggaagg cgaggcggtc gttgaagtcg taagtgatta tcgtgtcgaa
     1681 gaagtggcgt ttgtggcggc ggtcgccggg ctgctcggcc ccgaagatgt agttctgcgt
     1741 cagggagaac ttgtttgtcg ggttgagtgt gagggcgagt gcgacggtct tgccgccgtt
     1801 gttgtcttcg acgttattcc aaccgttgac gacggcgccc ataacggtga ccttcgggcc
     1861 gaacgagtat gtggcgcgca gtcccatgtg gtaggtgggc gaggcgaacg agtagagcag
     1921 gccgcgtgaa taattccagt tctccagaac ctgatcggat tcggcgccgc tccacgagat
     1981 gagctttccg aagtcgagct ggagaccgga accgacgggc gcgagatagc tgccgtaggc
     2041 ttgatggatg tgtttataga cttcgatccc gcccggctcc gccgacgcga ggtagtccgc
     2101 cgccggcccg aagaggaggt cgagtcgata gcccacacgc gaggccgcgt ccggcgtctt
     2161 ctcgaaggcc agcccgacgg cggcgagcga gaactgattg tggcgagtgt cgaaagcccg
     2221 cagcgagttg agcgcgtcat gcggccggtt aaagttgtag gcgtaatagg tgtcgatgta
     2281 gccgttgacc gtggtcgatt tgaaaaaccg gagcagttcg ttctcttgag gctgttcctg
     2341 acgcggccct ccgcttgagg cagccggctc gtcctccggc tcgcgcgctc tttgcaccga
     2401 cgttgcgccc gcatctaacg ctcccgcgtc acgccccggc ttcagcaagg cttcggccct
     2461 ttgcaaacgc tcttcgagtt cgcggacgcg ccggcgcaac tcgacattct ccacctccgc
     2521 cgcgcttgcg gccggctgct gagcctgcgc acataacggc gagacgcaca gcagcgcgct
     2581 caggcagaag gcgacagtcc gtttcaacaa ctcaacgcgg gtgatgaatg taggggcagg
     2641 caagtccgcg gcccgtcttg tcggaggaat aattaacatg gatggacagg atgtgaagga
     2701 tgaagaaagg gctgatttca gatttgagat ttccaatctt ctgatcctgc tcatcttgtc
     2761 catccatgtt aattgcttcc gcgactcgcg ggcagggata agccctgccc ctacagtttt
     2821 gcaacggaca ctaatgatca ttctccgcct ccatcatgcc cgaacccact cgcccggcgg
     2881 gcggctagag tagcagcgtc tcgacgcgat gcgcgtgtac catctcttca cacggcgacc
     2941 acgcgaagac cgtgaagcgt ccgtcctgcg acgcgacgag cgcgaccgag tcatgctgat
     3001 cctgtacgaa ctgcgccgcc gacatgtggc gcgtcccgcc gacctgtatg ggggggacga
     3061 ccgaggacac gccgtcgatc accggctccg tcaaaaccac ctgctcgacc tgtgggtaac
     3121 ccttgcggcg cccgatcttc gcgccgaacg cgagtatctc gtattgatcg ctgatgacgg
     3181 tcgcgccgtc caccgccgtc aggcccgcga cggcctcgac cgcatcgatg agcgcctctt
     3241 gccacagtcg cttgtttctc tccgccgggt cttgccggat gagttcggcc agctcggagt
     3301 aagggggcgc cacggagtag gggacgggcc agacgattga ctctcgccat gcttcggtgt
     3361 cttgcggcac gacgagcagc gacccgccgc gcccgtgcaa gcgcatggag gcggccagtt
     3421 ggacgagcac gttcaaggag tcctcaccgg aggcggacga gccgaagccg agcagagaag
     3481 tgacgagtgc cgggcagtcc ggcaggctgg tgcccgcctc gtccacttcc ttgatctggt
     3541 cgcccttgag caccacgacg ttgacgaact tgcctgactc ctgaccgcgt cggtacttga
     3601 ccacgagcag ccccggctcg atgacttcca gcacgaagca gaacctcgga atcgtgcggg
     3661 tggtgcccca cacgtgcagc ccttcgccgt taagccagac gccgaggtga atgccgggac
     3721 gctccaccgc cggcgcaagc cgcgccagag ccgccggggt gagcgaaagc ggttgctgga
     3781 acatgagcga ctgtccggcg cgtccgggcg gaaggaaggc gagagatatt ttcggcgaat
     3841 aaccttcctc gcgccggagg ctcgcccaga acgcggcgtc gatgatcgct tcgatgatct
     3901 cggcgtcggg tcgctgcgac acctcctcgg ggccgtactg tcgcaacgcc gcgaggtggc
     3961 gggcgaagtg cgcctgcacc atggcggcga ccgcccgcgc ggccgggtat gaagggccta
     4021 tcatagattg atgacctcga acatctcctt gatgaattct gtactcgttc cgtctgtgac
     4081 ttaagtataa ctccgatgtg cctctgtggt gagtctgtct tgccacctgt acaactgaat
     4141 tgctacaggg aaggtgcttg acctttcatc tgacaatgag gtgtagaagg gcttgtgcga
     4201 aggtcggggg cgggtgttat gcggcgtgaa cagcgaccgt gaatttgacg taatgcgagg
     4261 aggagggctt ccacgtcatg gtgtttctgt tagccgccgt cctgctcccg accgcgtggc
     4321 aatacattct catggcccgt cggcgagggc tgctctacgt cgtcaaaagc atcgcggcgg
     4381 gggcggcgct cggcctgctt gcgggagtct tcatcggcgt cggggcgcgc acggggatga
     4441 gcgccattac cgtcgccaat ggcgacgcgc cgagcctcac gctgtccggt tcatttaccg
     4501 tcgtctacac gttcgcaagc tttggcatcg tgctgggcgt cgtgtacgac ggactgttcc
     4561 gacgcctgct gagacggcgc ggtttggtct acggggttct attgatgctg tgctcgtggt
     4621 atccgctcgc gcaggccgcc ggccaacaac tcacgggacg cccggccctc atcacacaaa
     4681 tcatggtgtc cggcgttatc gtcgcgctga tgtggctacc gttcggagta gctctggaag
     4741 cactcttaag caggtggcac aatcgtcgcg ccgcgtcggc ggcagcaagc gccgctgtct
     4801 gacccactca ctcgtcgggg ttcaactcac tcgtatctca acgccaccat cgggtcaacc
     4861 ttcatggcgc gtcgcgcggg gatgtagcag gcgaggagcg ccacgcccat caggagcagc
     4921 gcgatggccg cgaatgtcgt ggggtcgctg gcgcttacgc tgtagagcaa ggtcgccatc
     4981 agtcgcgtca gcgcgagggc ggcgcccaga ccgatgacga tcccgatgag cgccagcagc
     5041 aggccctgcc ccagaaccat cttgagcacg tcgcgcgcct gcgcgcccag cgccatgcgg
     5101 atgccgatct cgtgcgtgcg ctgtgagacg gcgtaggaca tcacgccgta gatgccgacc
     5161 gaggccagca cgagcgccac cgccgcgaag acgccgaaca gaatggcgta gagacgcggc
     5221 tgccagaccg accttgcgac gacctccttc atcgtcctca cgttggtcac aggctgatcc
     5281 gggtcaagct ctttgacacg ggcgcgcacg gccgtcgcca gcgcggcggg gtcggacgaa
     5341 gtacgcgccg tgagcgtcat ctgtggctcg ggtatctgct ggtaggagac gtagacggtc
     5401 ttccgtgtct gccggtcgag gcgttcgtgg cgcacctccc cggccacccc gacgaccgtg
     5461 aaccacggct cgttgctctc tggcggccca aaccggacgc gcttgcccac ggcgctttcc
     5521 tccggccagt actcgcgggc cagtcgctcg tcgatgaccg tgaccttgag cgcgtctttc
     5581 gcgtccgtct ccgagatgtc gcggcccgtc atgatgggaa tgcccatcga gcgaaagtag
     5641 tcgggggtga tgacgcagta gttgatcatc ggcgcctccc cgacggagag caccgggcga
     5701 ccctcgaccg tgaggcttcg gccccagagc gacccgctca gcggcaggtt cgaggccgcg
     5761 cccgccgcct ctacgcccgg cagcgccctc gtgcgttcga gcaactgccc gaagaatgcc
     5821 gggcgcttct cctgctcttt gtacttggcc cgcggcagcg agacggtcat cgtcagcacg
     5881 ttctccgggt tcaaccccgg attgacgtgt tgcagacgca ggaagctacg catcatcagc
     5941 cccgcgccga tgagcagcac gagcgagagc gccacctccg cgaccaccag cagcgagcgc
     6001 acgcgcccgt gccccgcccc cgccgccgcg ctgcgaccgc cctccttcaa cgtctcgttt
     6061 agatcgacct tcgatgcctg caacgccggc gcgatgccga agacgacgcc tgtcaggagc
     6121 gacacgccgg ccgtgaaggt gagcacgcgc cagtccagat tgaacttcat ccagaagggg
     6181 aactcgatag ggatagccgc cagcaaaagc tccagccccc acaccgccag caccagtccg
     6241 agtgcgccgc ccgcaagacc cagcacaagg ctctcggtga ttaactgtcg cacgatgcgc
     6301 cagcggccgg cgccgagcgc ggcgcggatg gaaatctcct tctgccgcgc cgaggcccgc
     6361 gccagaagca ggttggcgac gttcacgcag gcgatgagca tcacgaagcc gaccacgccg
     6421 agcaggatca gaagcgcctg tcggtagtcg ccgaccaacc ctttgtgcag gccggagacg
     6481 ctgacgctca agccctcgtt agtgaccggg ttctgctcct cgatgcgcgc ggcgatagcg
     6541 gccatctcgg cctgcgcctg ttcgagcgtg acgccgggct tgagccgcgc caccgcgtcg
     6601 aggccgtgat cgttgcgcgt ccacctctgc tcgtcgagcg ccatgggaat ccataggtcg
     6661 gcgacttcag ggaatcggaa gtcaggcggc atcacgccga taactgtgtg ctggcggttg
     6721 ttgaggctaa tcgtccggcc gacgatttcg gggttcgcgc cgaagcggct ctgccacagg
     6781 ccgtggccga ggatgaccac cgtgtcgtta tcggggcgat cttcttcggc gcgaatcgtc
     6841 cggcccatcg tcggggccac gcccagtatc tcgaagaggc cgctggaaac tctcgccccc
     6901 ctcatctgct ccggctcccc gtcacccacc agtgtgtagc tgcccgcgtt gtaggcggcg
     6961 atgtcctcga agacgcggtt ctgctcgcgc cagtcctcga agttcgggaa ggacacgccc
     7021 atcgcgttga cgccgcgctt cggggccgtc tcgtcgagca gaaccagtcg ccccggctcc
     7081 ctgtagggaa gcggacgcag cagcacgccg ttgacgaagc tgaagatggt gctgttcgcg
     7141 ccgacgccga gggccaccgt cagcaccgcg acggccgcga agccgggctg cttcaacagc
     7201 gagcgcacgc cgtatcggat gtcctgccag aaagtttcca caatgcctcc tccacgcacc
     7261 tcgtaggccc gctccgccat gcggccgggg ttgccgaaac ttctccgcgc ggcgaggcgc
     7321 gcctcctccg gcgtcatccc ccggccgatg ttcgcctccg tctccatctc gacgtggagg
     7381 cgcaactctt cctcgatgtc gctgatgacc gcctcgcggc cgaagagggc acgcagtcgc
     7441 gccgagatga cgttgaacca ctccatccgc cacacactcc cttacgtggc ctgtctcacc
     7501 ttcgcaatcg ccaacgccag ccggtcccaa ctctcctcct cgatcttgag ctgccgccgg
     7561 ccggccctcg tgagcgagta gaatttggcc cgccggccgg tctcattcgt gtcccactcc
     7621 gaagtcagcc agtcgcgctg ctcgatgcgg tagagggccg ggtagagcga cccctgctcg
     7681 acgttgagca tctcctcgga catctgccga atgcgctgga tgatgccgta gccgtgcaac
     7741 ggcccgaggg agacggccct caggatcagc atgtcgagcg tgccttgcaa cagatcggct
     7801 ttaacttgac tcacttgcag ccctccgggt gaattcgcgt aaaacggctt gatgcctaga
     7861 acgacgaggc atcgtagttc cgcacgccta ggatgtcaag gctttcccgc gcgatgagca
     7921 ttcaggcgag tcggaggagg tgaaagtgaa gtggatgctt gtgaggcgtt agcccggagg
     7981 acgtgtattt cgctgagtgc gggttggaga ttgaatcggt aaagtttttc agttcatggt
     8041 tagacgtggt cttcccggtc tcgtttcggg gcgggcggca gttggccatc tcgcgctccc
     8101 gaaagtaatg cagtggtctt ctccgaaggc acacgcattg aatgtgacgc ttccatatct
     8161 gacagcgcgc cggtttgcgg attacccacc ggcaacacga cacccgtttc acctttggga
     8221 ttcagtgtgt aatgtaaacg ccgcagcccc gtcaagagta acgcccaacc caatgctccc
     8281 agcaggagcg acactccaac aacgacattt gtgcgcggca atgctgctgt ggtcatgccc
     8341 agcaacaacg caatgaaagt gagtgcgagg atgattgcgc caaacacggt gcgggtgtag
     8401 cccgcgcaca gtccggcggt cactttcagc acttcgactt ccgccggttc tgatgacacc
     8461 gtcggcgtcc cgcactggcg gcagaaacgt gaacccaccg cggccgtcgc gccacaggcg
     8521 acacacccgg ccgacgccga gcgcatcgcg gcaagggcga tcatcgcttc cgcctcgcgc
     8581 gtcagcgtct gccgaccgct attcgtcaac atccgatgct tgagcgcata gcagaaggtg
     8641 atgcgtgcaa ctccggcgct caaaggtttg acggcgatgg tcagagcgac cgagtaattg
     8701 aggacgttgt tggaaccgat ccacgaagcc cagccacgcg ccgcccgtcg agctactaac
     8761 ggctcctctt tcagcacgta gtaaccgagc tgctcaagcc cgtcggcaag gcgcgaacgc
     8821 acactttcca cgtctccaag caagatacgc gagaggaggt gctcgccggc gccggacgcg
     8881 ccggcatcat tcaacgccgc gaccgaatca ctatccatca gcgttctcct cgcctgctcc
     8941 actctctcct gccctgtgtc ctcttacact tggtgggaag acggcccgcc gcgctcagtc
     9001 tccgaccact tctcaaccgg ctgggccgcg tgcgccgtca tgtccgcgtt gaggcattat
     9061 agcatcggcg ctgagggggc ttcgcgtcga tgccggaggg cgactgtcag gtctttcgca
     9121 acagttgggg cacaggcttc atggagcacg gctgcggcta cataactgtg ggtagagtgt
     9181 gatgacgcgg ccggggcttg tcagcgcacg gcgcgcgacc gtatgctgtg gttggccgca
     9241 tggacgccga gaggctggac gggctgaagg tcaatggccg ccggcggcga ataattcggc
     9301 cgacacactt tccgacttac acacgcagtt gaaatgttcc cggacgaaac gaactttggc
     9361 gcgcgcgcta aatttaggga agggaagggg caacgacgcg cccgcggcgc cgcatcacag
     9421 ccccgggatg gaactcggtg acacgtcacg acttccgg
//
LOCUS       DASVES010000171         6338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1600614, whole genome shotgun sequence.
ACCESSION   DASVES010000171 DASVES010000000
VERSION     DASVES010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600614"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..614)
                     /locus_tag="VF240_06920"
     CDS             complement(120..614)
                     /locus_tag="VF240_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7174996.1"
                     /translation="MKTLFVLRHAKSSWDNPGQKDFERPLNERGRRDAPRMGRLMRER
                     APRPDVILCSPAERTRQTVALFATAAGLDAEPRFDERIYEASAGRLIDVVSEVERGVG
                     SVLLVGHNPGVEELIEALTGSRERMTTAALARVALDIDAWDEVGPECGRLEWVVRPKE
                     LGDG"
     gene            844..1512
                     /locus_tag="VF240_06925"
     CDS             844..1512
                     /locus_tag="VF240_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7174997.1"
                     /translation="MTKSERTIVVGDVHGCYDELTELLERAAFGAGDRLVCVGDLVTK
                     GEKSREVLDLFIRDARLSSVLGNHDRAVLRALRGEAVALKPAQEKCLQELQPEGARYA
                     QFLSTLPLSIDLGSHVVVHAGLRPGVALAEQSADDLTELRTLGPDRTNREGTPWYDVY
                     DGERTALFGHWPAPVPRRGLRAIGLDTGCVYGYELTAYVIEEDKFLSVPARRAYSPPG
                     GRVG"
     gene            1629..2765
                     /locus_tag="VF240_06930"
     CDS             1629..2765
                     /locus_tag="VF240_06930"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX7174998.1"
                     /translation="MAKAREIEGLDCGADAASGIRLVLSSRIDETLAYRDAALVAEDP
                     EGVHDMRVASRRLRSALRDFAPHMNDKRVAGVRVELKKLADALGRVRDIDVEAAALEE
                     AAREAPETVRAGVEELARARLAGSAEAREELARELGEDGLARLREEFADALANALDAA
                     RERRKGKGGGGAGRRGGSFGEVGRQVIVARWEELRERAGSLYRPFKTKRLHKTRISAK
                     RLRYALELFTPCMGDELKDFAREVAHLQKSLGEVHDCDEWIASLGVGLAKRERDAADD
                     AKSPDAGARAGRAETSAWLFDHFVERRNLHYREAFARWRAWGREDFESRLRACVSGRG
                     SEGVRAAAEGEAAEGVDGEEPGKRRPRRSAKASRGGRESSKVAN"
     gene            3013..4386
                     /locus_tag="VF240_06935"
     CDS             3013..4386
                     /locus_tag="VF240_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7174999.1"
                     /translation="MRYKENILGLVGNTPLVRLNRMAGGLKAQVFAKMENLNPGYSVK
                     DRIGIAMIEAAEADGALKPGGTIVEATSGNTGIGLALAAAVKGYKCIFVMTDKASTEK
                     VRYLKALGADVVITPVAAKPGTPDHYVSTAQRIHRETPNSFYPNQYAHPANPEAHYRT
                     TGPEIWEATEGKITHFVSGMGTCGTISGAGRFLKEKNPAIRIIGADPYGSVFKTYKET
                     GKTHEVTPYLVEGIGQEVIPENCHIKYVDEVINITDRESFEMSRQLGRQEGIFCGGST
                     GTICAAALKLARTLDETGVVVFIVCDTGEHYLTKHHSDEWMKEKRLLETQKMTAGLIS
                     TTKGERGPGKLVAAAPDELVSAALAKMGEHGLTQLPVVDDGRSVGSLRENRLLAKILK
                     NRELMEAPVREVMEKGFPVVDEDAAWAEVLTHLHSSPAVLVGEYGRITGIITRHDVLD
                     APAGSRG"
     gene            4558..5760
                     /locus_tag="VF240_06940"
     CDS             4558..5760
                     /locus_tag="VF240_06940"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7175000.1"
                     /translation="MTYIVLIAWAIAFAWLLGAALTLAALARLKFLKPTDDSRLKTED
                     APHVSILLPARNEERRVLADCVRSLLAQDYGSFEVVAVDDRSTDATLPLLRSLAEEDA
                     RLRVIAGGELPDGWLGKPNALRQALETARGSWVLTTDADVIFHPSALRTALTYARSHD
                     CDAVSLFPHFETRTFWERVFTPTWVWGLLVFFPLDLVNRRRGVRLAIGMGAFVLMRRE
                     ALAAIGDFASVRADVIEDVRLAEKLKRSGAYLRVEFAPDLIRTRMYSNLRELWEGSTK
                     NLFAALKFSYALTLAYLVWTFSVAVGPTILLGLSATLITAGVEGPWRELLVPSSLALA
                     LHVALLALVCVRFRVPPAYALAGPLGFALACAVMLTSAVAVTTGRGVTWKGRKIYERA
                     GGLRPPRA"
     gene            complement(5777..6256)
                     /locus_tag="VF240_06945"
     CDS             complement(5777..6256)
                     /locus_tag="VF240_06945"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX7175001.1"
                     /translation="MSKIIGIVAVDRNGAIGRGGALPWHYPADMKFFREQTTGNVCVM
                     GRRTWASLKKPLKDRLNVVLTTRGEVEPHPTVVVLRDRASVLSLKGYLACDIYVIGGA
                     RVYESFLADIDAWVVTEVPIAVEGADTFMPVDFLSGFTPSDTRQLDDDLKVIFYERR"
BASE COUNT         1099 a         2106 c         2108 g         1025 t
ORIGIN      
        1 ctcacggaga tcgacgcgtg gggacgcgcc aacaacatcg ccctctatcg cctccggccg
       61 gaagtgttgg acgggcgcaa gacggccgaa tagtcgctcg cgggtggcgc ggccgtgtct
      121 cagccgtcgc ccagctcctt cggcctgacg acccattcga ggcgaccaca ctccgggcca
      181 acctcatccc acgcgtcgat gtcgagggcg acgcgggcca gcgcggcggt cgtcatgcgc
      241 tcgcgcgagc cggtgagcgc ttctatcaac tcttccacgc cggggttgtg gccgacgagc
      301 aacacactcc ccaccccccg ctcgacttcc gacaccacat cgatcaggcg accggcgctc
      361 gcctcgtaga tgcgctcgtc gaagcgcggt tcggcgtcga ggcccgcggc cgtggcgaag
      421 agcgcgacgg tctgccgcgt tctctcggcg ggtgagcaga ggatcacgtc gggccgcgga
      481 gcgcgctcgc gcatgagtcg gcccatccgc ggcgcgtccc tgcggccgcg ttcgtttagc
      541 gggcgctcga agtctttctg ccccggatta tcccaactcg atttggcgtg tcgcagaacg
      601 aaaagcgttt tcatcgctgg cggaattcca ccttcctcgt agcggtcata taactccaaa
      661 cttgattata gggcggctcg ggctcaccgc agaggcgcag agggggcgca gaggcgttga
      721 atcaactcaa gcctctgcgg tgaactcatg cacgctacac acacgcatcg ggtctcctga
      781 aaataccacg atgtgtttgt taccatcgcg gccggtatgt tgtaatccac tcgcggaggg
      841 gaagtgacga agagcgagcg cacaatcgtc gtcggggacg tacacggctg ttacgacgag
      901 ctgacggagc tgctggagcg cgcggcgttc ggcgcgggcg accgtctcgt ctgtgtgggc
      961 gatctcgtca caaagggcga gaagagccgc gaagtactcg acctgttcat acgggacgcg
     1021 cgcctctcct ccgtcctcgg caatcacgac cgcgccgtgc tacgcgccct gcgcggcgaa
     1081 gctgtcgcgc tcaagcccgc gcaggagaag tgcctgcaag agcttcaacc cgaaggcgcg
     1141 cgctacgcac agtttctctc gacgctgccg ctctccatcg acctcggctc acacgtcgtt
     1201 gtacacgccg gcctgcgtcc cggcgtcgcg ctcgcggagc agtcggcgga cgatttgacg
     1261 gagctgcgca cgctcggccc cgaccggacg aaccgcgagg ggacgccgtg gtacgatgtt
     1321 tacgacggcg agcgcaccgc gctcttcggc cactggcccg cgcccgtgcc acgacggggc
     1381 ctgcgcgcca tcggtctcga caccggctgc gtctacggct acgagctgac ggcctacgtc
     1441 atcgaagaag acaaattcct gagcgtcccg gcccgccgcg cctacagtcc tccgggcggg
     1501 cgcgtcggct gaatgagttg tcagtagtca gttgctttcc gtttccaatc ttgagcatta
     1561 acctttagcg cggtgtttac caaacaccta taactgatta ctgactactg acgactgact
     1621 gctaaccaat ggccaaagcc agagagatcg aagggcttga ttgcggcgcg gacgctgcca
     1681 gcggcatccg gctcgtactc tcctcgcgca tcgacgaaac gctcgcctat cgcgacgccg
     1741 cgctcgtggc ggaagacccc gaaggcgtcc acgacatgcg cgtggcctca cgccgcctgc
     1801 ggagcgcgct ccgcgacttc gcgccccaca tgaacgacaa acgcgtggcc ggcgtccgcg
     1861 tcgagttgaa gaagctggcc gacgctctgg ggcgcgtgcg cgacatcgac gtggaagcgg
     1921 cggcgctcga agaggccgcg cgggaagcgc ccgagacggt gcgcgcgggc gtcgaggagt
     1981 tggcgcgcgc gcgtctggcc gggagcgcgg aggcgcgcga ggagttggcc agggagcttg
     2041 gcgaggatgg tctcgcgcgt ctgcgcgagg agtttgccga cgcgctcgca aacgcgctcg
     2101 acgcggcgcg tgagcgtcgg aaggggaaag gcggcggcgg cgcgggccga cgtggaggga
     2161 gcttcggcga agtcggccgg caggtgatcg tcgcgcgctg ggaggagttg cgggagcgcg
     2221 ccggctcgct ctaccgcccg ttcaagacga agcgccttca caagacgcgc atctcggcca
     2281 agcgcctgcg ctacgcgttg gagctgttca cgccgtgcat gggcgatgaa ctcaaggact
     2341 tcgccaggga ggtcgcgcat ctgcaaaagt cgctcgggga ggttcacgac tgcgacgagt
     2401 ggatcgcgtc gctcggcgtc gggttggcga agcgcgagcg cgacgccgcc gacgacgcaa
     2461 agtcgcccga cgcgggcgcg cgcgcgggac gtgcggagac ttccgcgtgg ctgttcgacc
     2521 acttcgtcga gcggcgcaat ctgcactacc gcgaagcgtt cgcacgctgg cgcgcgtggg
     2581 ggcgtgaaga cttcgagtcg cggctgcggg cgtgcgtcag tgggcggggg tcggagggcg
     2641 tgcgcgccgc cgcggagggc gaggcggccg aaggcgtgga cggagaagaa ccggggaagc
     2701 ggcgcccgag acggagcgcg aaagcgtcgc gcggcggtcg cgagtcctcc aaagtcgcga
     2761 actgaatcgt cgctcaaagc cgcgaattga gtcatcgctc tctccgtcgc tcaaacgacc
     2821 ggaggcgtcc ttccgctcaa tgcgtcttcc cacagcccgc gacgtggaac acaaccttcc
     2881 ggcgcgcgcc tcggccgtgt gagactccgt cgcgcgtggg tcgtctcgcc tgaaagtggc
     2941 agggcggcga gattccgttg tataaatgtt gccgcacaca tccacacgaa caaaggcgcg
     3001 ccggagattt cgatgaggta taaagagaac atcctcgggc tggtcggcaa cacgccgctc
     3061 gtccgtctga accgcatggc cggcgggctg aaggcgcagg tcttcgccaa gatggaaaac
     3121 ctcaaccccg gctactcggt caaagaccgc atcggcatcg ccatgatcga agcggccgag
     3181 gccgacgggg cgctcaagcc gggcggcacc atcgtcgagg ccacctcggg caacaccggc
     3241 atcggcctcg cgctggccgc cgccgttaag ggctacaagt gcatcttcgt gatgaccgac
     3301 aaggcatcga ccgagaaggt gcgctacctg aaggcgctcg gggccgacgt ggtcatcacg
     3361 cccgtcgccg ccaagccggg cacgcccgac cactacgtct cgaccgcgca gcgcatccac
     3421 cgcgagacgc ccaacagctt ctacccgaac cagtacgcgc accccgccaa ccccgaggcc
     3481 cactaccgca ccaccggccc cgaaatctgg gaggccaccg aaggcaagat tacgcacttc
     3541 gtctcgggga tgggcacctg cggcacgatc tccggcgccg gcaggttcct caaggagaag
     3601 aacccggcaa tccgcatcat cggcgcagac ccctacggct cggtcttcaa gacctacaag
     3661 gagacaggca agacgcacga ggtcacgccc tatctcgtcg aaggcatcgg acaggaagtc
     3721 atccccgaga actgccacat caagtacgtc gatgaggtca tcaacatcac cgaccgcgag
     3781 tcgttcgaga tgtcgcggca gctcggccga caggagggca tcttctgcgg cggctcgacg
     3841 gggacgatct gcgcggcggc cctcaagctc gcgcgcacgc tcgacgagac gggcgtggtc
     3901 gtcttcatcg tctgcgacac cggcgagcac tacctgacga agcatcactc ggacgagtgg
     3961 atgaaggaga agcgtctgct ggagacgcag aagatgaccg ccggcctcat cagcacgacg
     4021 aagggcgagc gcgggccggg caagctcgtc gcggccgcgc cggacgaact cgtctcggcg
     4081 gcgctggcga agatgggcga gcacggtctg acgcagcttc ccgtggtgga cgacggccgc
     4141 tcggtcggga gtctgcggga aaaccgtctg ctcgcgaaga ttctcaagaa ccgcgagctg
     4201 atggaagcgc ccgtgcgcga ggtgatggag aagggcttcc ccgtcgttga cgaggacgcc
     4261 gcctgggccg aggtgctcac acaccttcat tcaagccccg ccgtcttagt cggtgagtac
     4321 ggccgcatca ccggcatcat cacgcgccac gacgtgctcg acgcgcccgc cggctcgcgc
     4381 ggctaacgtc gcgggcggtg agtagtgtcc taagtcgatg atgttctctg cgcggcctct
     4441 gcgccctctg cgcctcggcg gtgaataact gcccggccac actcaccgca gagccgcgga
     4501 gggcgcaaag gccgcgcaga gagttgattt taggacacga ccgggcagtg acgccgattg
     4561 acttacatcg tactgatcgc ctgggccatc gccttcgcct ggctgctcgg cgcggccctc
     4621 acgctcgccg cccttgcccg cctcaagttc ctcaaaccga ccgacgactc ccgactaaaa
     4681 actgaagacg cgccgcacgt ctcgatcctg ctgcccgcgc gcaacgagga gcggcgggtg
     4741 ctcgccgact gcgtgcgttc gctgctcgcg caggactacg ggagcttcga ggttgtagcc
     4801 gtggacgacc gctcgacgga cgcgacgctc cccctgctgc gctcgctcgc ggaagaggac
     4861 gcgcgcctgc gcgtcatcgc gggcggcgaa ttgcccgacg ggtggctcgg caagcccaac
     4921 gcgctccggc aggcgctcga aacggcgcgc ggcagttggg tgctcaccac ggacgcggac
     4981 gtgatctttc acccgtcggc gttgcggacg gccctgacat acgcgcgctc gcacgactgc
     5041 gacgccgtct cgctcttccc gcatttcgag acgcgcacgt tctgggagcg cgtcttcacg
     5101 ccgacgtggg tctggggtct gctcgtcttc ttcccgctcg acctcgtcaa ccgccgccgc
     5161 ggcgtgcggc tcgccatcgg catgggcgct ttcgtcctga tgcggcgtga ggcgctggcg
     5221 gcaatcggcg attttgcgtc cgtccgcgcg gacgtgatcg aggacgtgag gctggcggag
     5281 aagctgaagc gttcgggcgc atacctgcgt gtggagttcg cccccgacct catacggacg
     5341 cggatgtact cgaaccttcg ggagctgtgg gagggcagca cgaagaacct cttcgcggcg
     5401 ctgaagttct cttacgcgct cacactcgcg tacctcgtct ggacgttctc ggtcgccgtc
     5461 gggccgacga ttcttctggg attgagtgcg acgcttataa cggccggcgt cgaaggcccg
     5521 tggcgcgagt tgctcgtgcc gtcgtcgttg gcgctggcgc tgcacgtcgc gctgctcgcg
     5581 ctcgtctgcg tgcgcttccg cgttccaccg gcctatgcgc tggccgggcc gctcggcttc
     5641 gcgctcgcgt gcgccgtcat gctcacttcg gcggtggccg tcacgacggg gcgcggggtg
     5701 acgtggaaag gacgcaaaat ttacgagcgc gcggggggcc tgcgtccgcc gcgcgcgtga
     5761 gggcgcgaaa cgctcttcag cgacgctcgt aaaaaataac cttcaaatcg tcgtcgagct
     5821 gccgcgtgtc ggagggagtg aacccgctca gaaaatccac gggcatgaac gtgtccgcgc
     5881 cctcgaccgc gatggggact tcggtgacga cccaggcgtc gatgtccgcg aggaacgact
     5941 cgtagacccg cgcgccgccg atgacgtaga tgtcgcacgc cagataaccc ttcagcgaga
     6001 gcacggaggc acggtcgcgc aggacgacaa ctgtgggatg cggctcgacc tcgccccgcg
     6061 tcgtcagcac cacgttcaac ctgtccttca gcggcttctt caacgacgcc cacgtccgcc
     6121 gccccatcac gcacacgttg cccgtcgtct gctcccgaaa gaacttcatg tccgccgggt
     6181 agtgccacgg cagcgcgccc ccgcgcccga tggccccgtt cctgtcaacc gcgacgatgc
     6241 cgatgatctt agacatagtg atgagtgatg agtgatgagt gataagaagg agaagacagg
     6301 ttttgtctta tcacttatca ctcatcactc atcacttg
//
LOCUS       DASVES010000172        11739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_284411, whole genome shotgun sequence.
ACCESSION   DASVES010000172 DASVES010000000
VERSION     DASVES010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284411"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF240_06950"
     CDS             complement(<1..347)
                     /locus_tag="VF240_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175002.1"
                     /translation="MERESIEMDVVFVGAGPANLSGALHLARLIKEHDEAVGAGKREG
                     KPLGEIQIAVIEKGATIGAHILSGAVMDPKALAELMPDFVEQGAPLESPVTEDYFLYL
                     TKNRAIKSPITPPP"
     gene            855..1799
                     /locus_tag="VF240_06955"
     CDS             855..1799
                     /locus_tag="VF240_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175003.1"
                     /translation="MFWSGLTSLLRVIIYGWVIALWQFLKLLCQFCRKRCARPPKTPL
                     ECLQIDHPAFVRPDPLLYSQRYLKAHGFAVTYNNPDISLSKGGVLVSSHDLEPATTYE
                     VTARIWNNSLEAPVVGMPVHLSFRNFGVGNQPIPIASGAVDVGVKGSVTHPGFVIIPW
                     TTPATPGHYCLQVLLDPVDDIERLNNLGQENTDVRKAHSPALFEFTLRNDTPKSRRYH
                     FEVDAYEIPALPKCGDTRPDDAATLRRHRPEAHPVPAGFVVKIEPSTPTLDPGVSATI
                     NVSVEPPAGFVGRQALNINAFHEQGFAGGVTLTTVKEP"
     gene            1803..2858
                     /locus_tag="VF240_06960"
     CDS             1803..2858
                     /locus_tag="VF240_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175004.1"
                     /translation="MAVQQFTKCIEPERFEPRSRIKMIAMATAFAGVFAAYAVLTGHP
                     WCALFAVEIGWCMLWILYCENWLYERLICIGGDVDAIGLVVQVDPPKFTANLFDVDND
                     YCFNLLLQCTEFGVQEGDAALQNSPYGVLIEKKPVILALGSKVPGYKNGGYMVPGRPP
                     NSATLHCEMEGSVIHDYYLFMQAMLGITVLAFGVCVLLPHWISLLSWILAILAIVIVG
                     GMYESQVTHPGSPSDVNPDIATIHKDQDILYVHGTWVYDPLHDGYNEIHPVKICLKIG
                     CWNGDWTNTNCHPENGPTEYDKHCLPGGGAPDVILRLRNALEEARADVTIANQKLLEH
                     QWRFHPLIDACDRDVIL"
     gene            3431..5512
                     /locus_tag="VF240_06965"
     CDS             3431..5512
                     /locus_tag="VF240_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7175005.1"
                     /translation="MSAHKHTNRLADETSPYLLQHAHNPVDWYPWGDEAIEKARSENK
                     PILLSIGYSACHWCHVMERESFEDEKIAGLMNEHFVNIKVDREERPDLDQIYMNAVQL
                     MQRHGGWPLTVFLSPDLVPFFGGTYFPPEDRHGMPGFPRILSHLADAYRTRQSEIAEG
                     AAELLDELRRMSMTRESDEPLTVSILDDAARAVAKTFDPRHGGFGGAPKFPAPMTLDF
                     LLRTHRRTRDPHLLEIVTHTCRKMAFGGIYDQLGGGFHRYSTDAHWLVPHFEKMLYDN
                     ALLSRLYLHAYQATGDELFKRIVEETLDYVVREMTDPSGSFYSTQDADSEGIEGKFFV
                     WDEAELKDLLGEDDARLFSTFYNVTDEGNFEEENILNVTRPAEAVAADAGVSVEKLDE
                     VLRRGREVLFAARERRVKPGRDEKVLTAWNALMLEGFAEASATLGREDYRAVAERNAQ
                     FILENLKDGEHLLHVYKDGRAKHKGFLDDYAYFVSALVTLYETTGTLRWLQEATALCE
                     KMIDEFWDEEEGGFFYAGRSGEQLIVRTKDFFDNATPAGNSVAADALVRLSLLTANED
                     YRRRATTIFRVTRDALTRHPSAFGQLLGALHFHISAPKEIAIISGSDTEAGESLLREV
                     WGRYIPDKVVAQAREGDEESARIIPLLQERPALGGRATAYVCQNYSCRQPVTDARELA
                     LQLEEGAGADS"
     gene            complement(5552..6391)
                     /locus_tag="VF240_06970"
     CDS             complement(5552..6391)
                     /locus_tag="VF240_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX7175006.1"
                     /translation="MHDDESALRKALDSFPSGGVVGLDTETFWDRSSNNRSSVSLVQL
                     AAAEGEILVVDALATGIEPLRPALESPELFMVAHNARFDRMVLSGAGVQTAGLIDTLQ
                     MARMTLRLVSYSLASVVEHLFGLPLDKTYQTSNWRRRPLSKAQVSYAALDALIVLRVY
                     EELKSRLTGEGRFEEALKASTLSNRPSQGARQKRPKIDLSPPLTAEEKRVVLKLKAWR
                     LERAQSENLPAYMVCQDRTLEHLVRVRPETLEALGGVYGLGSAKITRFGDDLLKALRD
                     ACS"
     gene            6755..8692
                     /locus_tag="VF240_06975"
     CDS             6755..8692
                     /locus_tag="VF240_06975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7175007.1"
                     /translation="MRNCTLVGSVATAAAVLYHAWASGGIAYFFLGSLIVLHAFWLYR
                     LSEQRLEEVRRAQAEKLQHLQEIAALHLNTIESLAIAIDAKDQTTHGHVRRTQVYAME
                     LGKLLEVTPTELEALRAGALLHDIGKLAVPEYILNKPGKLTAAEFEKMKIHTVVGGDI
                     IRRVNFPYPVEDIVRYHHEKWDGGGYPRGLKGDQIPLVARVISVVDFYDATRCDRPYR
                     MGMKREESIGLLQRMSGKSFDPRVVETFVENIDYLDSLIARQDIQEQVPSAPPAGMNT
                     SSPDAGLAPDHFAASHGDAGFRSIAEAQREVFALHEIAQTIGGSLNLQDTSTLVASKL
                     RSIVPFDTCVIFLIDEKTGKARAVHAAGEHAEAFASRSVSIGDGITGWVIANSRSMCN
                     TAPELDLAGVAAEVAAKIRGVLVAPLTREDGSFGAITLYSDKLSSYTAEHVRLLESVA
                     HHASNALNNALIYERTKESALNDALTGLPNSRALHLLLGQRMAECRRQKDTPLSVLTI
                     NIDDFKEVNNTYGHGAGDRVLAGAAEIIKRQVRQMDVLTRFAGDEFVAVMPTAPGDVA
                     VRVAERIRAAVEAHKFYVRTGRSIGIGVSIGVGNFPTDGETSDDLLMAADRNMRRDKS
                     ARKPNPELSLAAGLAPADALR"
     gene            8904..10034
                     /gene="pdhA"
                     /locus_tag="VF240_06980"
     CDS             8904..10034
                     /gene="pdhA"
                     /locus_tag="VF240_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX7175008.1"
                     /translation="MSELKVIPMKPRTSSAEAGAQGAQVREAAGTDAPVVPIEVHERR
                     RQEQQHRVEEIDRETLLRMLYQMILVRRFEEKSAEAYTLGKIGGFCHLYIGQEAVAVG
                     VISALRKDDYVLTSYRDHGHALILGTSPDEVMAELYGKAGGCSAGKGGSMHLFDADTN
                     FLGGHAIVGGQIPLATGVAFASKYRGTDQLAVCFFGEAAVNQGAFHESLNMAQLWKLP
                     CIYICENNQYGMGTAVKRAMSLHEVVEKAPAYDMASEFVDGMDVLAVREATQRAVERA
                     RADSLPTLLEVGTYRFMGHSMSDPGNYRTRAEIEKHQERDPIKLFADSLKKKQVIGDA
                     DLSEIEKRVKEEVERALKFADESPEPAPEELFTNIYANPLGK"
     gene            10172..11155
                     /locus_tag="VF240_06985"
     CDS             10172..11155
                     /locus_tag="VF240_06985"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX7175009.1"
                     /translation="MAIITMREALNQALAEEMRRDEGVFLMGEEVAAYQGAYKVSKGL
                     LEEFGEKRVIDSPITELGFAGLGVGAAMVGLRPVIEFMTFNFSILATDQIINSAAKML
                     YMSGGQFKIPIVFRGPNGSAYQVSSQHSQSLESWFAHFPGLKVVMPSTPADAKGLLKS
                     AIRDDDPVIFMEQERMYGLKGEVPDGDDFTIPLGVADVKREGKDCTIVARSLTVPIAL
                     KAAEEMEKQGVSCEVIDPRTIRPLDTETIIRSVKKTNRVVIVEESHPFCGVGAELSAQ
                     IMENAFDHLDAPVKRLSGVDAPMPYAKNLERLALPDVPRLVAAVREVCYMD"
     gene            11257..>11739
                     /locus_tag="VF240_06990"
     CDS             11257..>11739
                     /locus_tag="VF240_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7175010.1"
                     /translation="MATQVVMPKLSPTMEEGQLARWLKKEGDKVSVGEPIAEIDTDKA
                     TMEAQALTTGVLRKILVPAGETVPLGAIIAVIAEADEDISDILSKAGSAQATTARKED
                     TQEPVKTGGDGSETESSGVVPGGERTPATVEGQPIAGDVGGAPAAKDSDDSDDTLEKS
                     Q"
BASE COUNT         2475 a         3644 c         3360 g         2260 t
ORIGIN      
        1 ggcggcggcg tgatcggcga cttgatggcc cggttcttcg tgagatagag aaagtagtcc
       61 tccgtcacag gcgactccag cggagcgccc tgctcgacga agtcaggcat caactcggcc
      121 aaagctttcg ggtccatgac cgcgcccgag aggatgtgcg cgccgatggt cgcccccttt
      181 tcgatgaccg cgatctgaat ttcccccagc ggcttcccct cacgcttccc ggccccgacc
      241 gcctcgtcgt gctctttaat gagccgagcg aggtgcaggg caccgctcag gttcgccggc
      301 cccgcgccca cgaacaccac gtccatctcg atgctttcac gttccatttc ttccacgctc
      361 cggtatcaat gctttaacgc ccgcacgcct gccgccgaat catccggcga ctattcaact
      421 gccgcgcacg ggtcaaaaat gaatcgccat tcagtttatc ttcgcccccg agaaatagca
      481 aaaggccggc aatgtgggtt cggttataaa gtgaaattcg taggtcataa agtgaaatcc
      541 ttgtagggtg gactccatca ggaatattta caagtgccat agcagttttg cgacccacgc
      601 actgaggctt gatttcaact tgatgtattg acttcttcta atgaggggga tattgtccgc
      661 gcgttatcat caatagctga gtccggagcc tctgcctccc aacccggaat cttatcgcca
      721 ctcatgtcga gcctgcggga caggatatct gaaaccacga tgtcgtaaag ctcgcgtcca
      781 gactatctat cgcgagacga caggcaactc ttacgtttca ttagttcaat tctggggccc
      841 caaaggagac catcatgttc tggtcaggat taacttcact cttgcgcgtg attatttacg
      901 gctgggtgat cgcgctgtgg cagttcttga aattgttatg tcagttctgc cggaagagat
      961 gcgcacgtcc gccgaagacc ccgctcgaat gtctccaaat cgatcatccg gcgtttgttc
     1021 ggcctgatcc attgctgtac tcacagcgct acctcaaggc acatggcttt gcggtgactt
     1081 acaacaaccc cgatatcagt ttgtcgaagg gtggtgtact cgtgagttcg catgatctgg
     1141 aaccggcaac tacatatgaa gtgaccgcgc gcatctggaa taactctctc gaagcgccgg
     1201 ttgttggcat gcccgtgcac ctgtccttcc ggaacttcgg tgtgggcaac cagcccatcc
     1261 cgattgccag cggcgcagtg gacgtcggcg tcaagggcag cgtcacccat ccgggttttg
     1321 tcattatccc gtggacgacc ccggcgacgc ccggtcacta ctgtctccag gtcttactgg
     1381 atcctgtcga tgacattgaa cgtttgaata atctcggtca ggagaatacg gatgtcagga
     1441 aggcgcactc gcctgcgctg tttgagttca ctcttcgtaa cgacaccccg aaatcgcgcc
     1501 gttaccattt tgaagttgac gcgtacgaga tcccggcgct gccaaagtgc ggcgacacaa
     1561 ggcccgatga tgccgccaca ctcagacgtc accggcccga agcgcatccc gtccccgccg
     1621 gcttcgtcgt gaagatcgag ccgtccacgc caacgctgga tccgggagtc agcgcgacga
     1681 ttaacgtgag cgttgaacct ccggccggct ttgtcggtag gcaagctctc aacatcaatg
     1741 cgtttcacga acagggattc gccgggggcg tgaccctcac cactgtaaag gagccgtgaa
     1801 ctatggcagt ccaacaattc acgaagtgca tcgagccgga aagattcgaa ccgcgctcac
     1861 gcatcaaaat gatagcgatg gcaaccgctt tcgcaggcgt ctttgcagca tatgccgtgc
     1921 tcaccggaca cccgtggtgc gcacttttcg cggtcgagat tggatggtgc atgttgtgga
     1981 tactctactg cgagaactgg ctttacgaac ggctgatttg cattggcggc gatgttgatg
     2041 ccatcggcct ggtcgtccag gtagacccac cgaaattcac tgcgaatctc tttgacgtgg
     2101 acaacgacta ttgcttcaac ctcctgctcc agtgcactga attcggggtt caggagggcg
     2161 atgcggcatt gcagaattcg ccgtacggtg tgctcataga aaaaaaacct gtgatactcg
     2221 ccctggggtc aaaggtgccc ggctataaaa acgggggcta catggtccct gggcgccccc
     2281 ccaactccgc gacgctgcac tgtgagatgg aaggttccgt catccacgac tattatctgt
     2341 ttatgcaggc catgttgggg atcaccgtcc tcgcgttcgg agtgtgcgtg ctccttcccc
     2401 attggatctc tttactcagc tggattctgg ccatcctcgc tatcgtgatc gtgggcggca
     2461 tgtacgagtc tcaggtgact catcccggct cgccgtctga cgtcaaccct gacatcgcca
     2521 ccattcacaa agatcaagac atactctatg ttcacggaac atgggtctac gatccactgc
     2581 atgacggcta caacgagatt cacccggtca aaatctgcct caaaattggc tgctggaacg
     2641 gcgactggac caataccaac tgccacccag aaaacgggcc cactgagtac gataagcact
     2701 gcctgccggg cggcggggca cctgatgtga tcctgcgcct gcgaaacgcg cttgaggagg
     2761 cgagagcgga tgtgacgatt gcgaatcaga aactactgga acatcagtgg cgttttcatc
     2821 cgctcatcga tgcgtgtgac cgggacgtga tcctctgacg ctgtcgcggg ctcaatgctc
     2881 caggacgttt ttgcggcagt ctcacgcatc gaagtcggtg atcttaattc cgcgttttaa
     2941 ggagaaagaa aacaaaacgc gtgtccgaag gaagggaatg tagccctcgc gagtcccacc
     3001 tctctcattg caatgcgcga atccctaatc cgtggtgagg aactgaaact aagatattcc
     3061 gagtacgaag agctttgatc aagggcggcg agtaaatttc aagtaatgac cgaatactgt
     3121 aggaaacttt catgttatgc cagaaaagat tttcacttaa ggaccgaatc tgccgcgtaa
     3181 gttattgatt ccagtaatgg gccggtttca ctttatgacc gaacccacag gcactttcaa
     3241 ttcggccaga tgggaacacg gattaaacgg atgagaggga taagcacgga tcaaatccgt
     3301 gcttatccct ctcatccgtt taatccgtgt tccccgcagt cccgtcgtga atgccgtttg
     3361 ccccgcgccg gcaaagggga cataattcct ccaacaattc caacaacaag cgacgagggg
     3421 aagcaatcat atgtcagctc acaagcacac caaccggctc gccgacgaga cgagtccata
     3481 tctgttgcag cacgcgcaca accctgtcga ttggtatccg tggggcgatg aggccatcga
     3541 aaaggcgcgc agcgagaaca agccgattct tctgagcatc ggttattccg cctgccattg
     3601 gtgtcacgtg atggagcgcg agtcgttcga ggatgaaaag atcgcggggt tgatgaacga
     3661 gcatttcgtc aacatcaagg ttgaccgcga ggagcgtccc gacctcgacc agatttatat
     3721 gaacgccgtc cagttgatgc agagacacgg gggctggccc ctcaccgtct tcctctcccc
     3781 cgacctcgtg cccttcttcg gcggcaccta cttccccccg gaagaccgcc acggcatgcc
     3841 cggcttcccg cgcatcctct cgcacctcgc cgacgcctac cgcacccggc aaagcgaaat
     3901 tgccgagggc gccgcagagt tgttagatga actccggcgg atgtccatga cgcgtgagtc
     3961 ggacgaaccc cttaccgttt ccatactcga cgacgccgcc cgcgccgtcg cgaaaacatt
     4021 cgacccacgg cacggcggtt tcggaggcgc ccccaagttc cctgccccga tgactctcga
     4081 tttcctgctg cgaacccacc ggcgcacccg tgacccacac ctccttgaga tagtcacgca
     4141 cacctgccgg aaaatggcgt tcggcggaat ctacgatcaa ctcggagggg gctttcaccg
     4201 ctattcgacc gacgcccact ggctcgtccc gcacttcgag aagatgctct acgataacgc
     4261 actcctctca cgcctctacc ttcacgccta tcaggccacg ggcgacgaac tcttcaaaag
     4321 aattgtcgag gagacgctcg actacgtcgt ccgcgagatg accgacccct cgggcagctt
     4381 ctactccacg caggacgccg actccgaagg catcgagggt aagttcttcg tctgggacga
     4441 ggccgagttg aaagacttgc tcggcgagga tgatgctcgt ctgttcagca ctttctataa
     4501 cgttacggat gaaggcaact tcgaggaaga aaacatcctg aacgtcacgc gtccggccga
     4561 agccgtcgcg gctgatgcgg gcgtgagtgt cgaaaaactc gatgaagttt taaggcgcgg
     4621 gcgtgaggtc ttattcgcgg cccgagaacg acgcgtcaaa cccggccgcg acgaaaaggt
     4681 gctgacggca tggaacgccc tgatgctcga aggcttcgct gaggcgagcg ccacactcgg
     4741 acgcgaggat tatcgtgccg tcgccgaacg caacgcacag ttcatcctgg aaaacttaaa
     4801 ggacggcgaa caccttctcc acgtctacaa ggacggccgc gctaagcaca aaggttttct
     4861 cgacgactac gcttatttcg tctccgccct cgtcaccctc tacgagacga ccggcacgct
     4921 gcgctggctc caggaggcga cggccctctg tgaaaagatg atcgacgagt tctgggacga
     4981 ggaggaaggc ggcttctttt acgccggccg gagcggggag caattgatcg tccgcaccaa
     5041 agattttttc gacaacgcaa cgcccgccgg caattccgtc gcggccgacg cgctagtcag
     5101 gctttcgctt ctcacggcca acgaggacta cagacgaagg gccacaacca tcttccgggt
     5161 gacgcgcgac gccctcacgc gacacccctc cgccttcggc caactcctcg gggctttaca
     5221 cttccacatc tccgccccga aggagatagc catcatcagc ggctccgaca cggaggccgg
     5281 tgagagcctt ctccgagaag tgtggggcag gtacatccct gacaaggtcg tcgctcaggc
     5341 gcgcgagggc gatgaagaat cggcgcggat aattccgctg ctacaggagc gccccgcatt
     5401 gggcggcagg gcgacggcct acgtgtgtca gaactacagt tgtcgccagc ccgttaccga
     5461 cgcgcgggag cttgccctcc aactcgaaga aggtgctggc gccgacagtt aaagaagctg
     5521 gtgagaaggt cgcgggggcg gggtgatatt tttacgaaca agcgtcgcga agggctttga
     5581 gcaagtcgtc accgaaacga gtaattttcg cggagccgag gccgtacacc ccgcccagcg
     5641 cctccaacgt ctcgggcctc accctgacga gatgttcgag cgttcgatcc tgacagacca
     5701 tgtaggccgg cagattctca gactgcgccc gctccaaccg ccacgccttg agtttcaaca
     5761 cgactcgctt ttcctcggcg gttagcggcg gcgacaaatc gatcttcggg cgtttttgtc
     5821 ttgcgccctg cgacggccgg ttgctcaagg tcgaagcctt tagcgcctct tcaaatctcc
     5881 cctcacccgt cagacgcgac ttcagttcct catacacccg taggactatc aacgcgtcga
     5941 gagcagcata tgacacctgc gccttcgaga gtgggcgccg tctccagttc gatgtctggt
     6001 aagtcttatc aagaggcagg ccgaagaggt gttcgacaac ggaggcgaga ctgtaggaga
     6061 cgagccgcaa cgtcatacgc gccatctgga gcgtgtcgat caggcccgcg gtttggactc
     6121 ccgcgcctga gagaaccatc cggtcgaagc gcgcgttgtg cgccaccata aataattcgg
     6181 gtgactcaag cgcgggccgg agtggttcga ttccggtcgc cagagcatcg acaacgagaa
     6241 tctctccttc ggcggcggcg agctggacga gcgagacgct tgacctgttg ttggaggagc
     6301 gatcccagaa agtctccgtg tctaaaccga cgacccctcc tgacggaaaa gagtcgagag
     6361 ccttccgcag agcactctcg tcgtcgtgca agcaatagcg aactccgtgt acctccccgc
     6421 cctcgcccat cttcgcagtc cttcctcgcc aatcggcgat tcttccgaca ccattgccgc
     6481 accccacgct cgcccgaaga ggatattatt tttcccccta agccgcacac atctttggtc
     6541 atgatgattg gtaagtattg catacaaatc ggaatctttc ccgacccgaa tcgcaaggat
     6601 aaatgcttga cctttaagga gaaaagcgtt atttttacat ggccaacgtt gttggcacat
     6661 ctgcttgctc cgcgaattct ctcctccccg gtcgaatcta ttcctggcct cttctccacc
     6721 agcgaaacct atcagccgca tgattcagaa gcgaatgaga aattgcacgc tcgtggggag
     6781 cgttgccacc gcggcggcag ttttgtatca cgcctgggcc agcggtggaa tcgcctattt
     6841 cttcctcggc tcactcatag tcctgcacgc cttctggctt taccggctca gtgagcagcg
     6901 tctcgaagaa gtgcgtcggg cgcaggccga gaagcttcaa caccttcagg agatagcggc
     6961 cttacacctg aacaccatcg agtcgctggc catcgccatc gacgccaagg atcagactac
     7021 acacggccac gtccggcgca cgcaggtcta cgcgatggag ttgggcaagc tgctcgaagt
     7081 tacgccgacc gagttggagg cgctgcgggc cggggcgctc ctgcacgaca tcggcaagct
     7141 ggccgtcccc gaatacattc tcaacaagcc gggcaagttg acggccgccg agttcgagaa
     7201 gatgaagatt cacaccgtcg tcggcggcga catcatcagg cgcgtcaatt tcccttaccc
     7261 cgtcgaggac atcgtgcgct atcaccacga gaagtgggac gggggcggct acccgcgagg
     7321 actcaaggga gatcagattc ccctcgtcgc gcgcgtcatc tcggtcgttg atttctatga
     7381 cgccacacgt tgcgaccgcc cgtaccgaat ggggatgaag cgcgaagaat caatcggcct
     7441 cctccagcga atgtccggga agtctttcga cccgcgcgtg gtcgagacgt tcgtcgagaa
     7501 catcgactat ctcgacagcc tcatcgcccg gcaggacata caggagcagg tgccctcggc
     7561 gccgcccgcg ggcatgaaca catcaagccc tgacgcgggg cttgcgcccg accacttcgc
     7621 ggcctcgcac ggcgacgccg gcttccgctc catcgccgag gcgcagcgcg aagtcttcgc
     7681 cctccacgaa atcgcgcaaa ccatcggcgg ctcactcaat ctccaggaca cctccacgct
     7741 ggtcgcgagc aagctgcgct ccatcgtccc gttcgacacc tgcgtcatct tcctgattga
     7801 cgagaagacg ggcaaggcgc gggcggtaca cgcggccggc gagcacgccg aagccttcgc
     7861 cagccgcagc gtctccatcg gcgacggcat cacgggatgg gtcatcgcca actcgcgctc
     7921 gatgtgtaac accgcgccgg agcttgacct cgcgggcgtc gcggcggaag tggccgcgaa
     7981 gattcgtggc gtcctcgtcg ccccgctcac gcgcgaggac ggctccttcg gggcgatcac
     8041 gctctactcc gacaaactct cttcctacac ggccgagcac gtccgcctgc tcgaatcggt
     8101 cgcgcaccac gcgtcgaacg cgctcaacaa cgcgctgatc tacgagcgga caaaggagag
     8161 cgcgcttaac gacgccctca cggggctgcc caactcccgc gcgctccacc tgctgctggg
     8221 gcagcgcatg gccgagtgcc ggcggcagaa ggacacaccg ctgtcagtgt tgaccatcaa
     8281 catcgacgac ttcaaagagg tgaacaacac ctacggccac ggggccggag accgcgtgct
     8341 ggcgggggcc gccgagatca tcaagcgaca ggtgcggcag atggacgtgc tgactcgttt
     8401 cgcgggcgac gagttcgtgg ccgtgatgcc gacggcgccg ggcgacgtgg cggtcagggt
     8461 cgccgagcgc atccgcgccg cggtcgaggc acataagttt tatgtccgaa cggggcgctc
     8521 aatcggcatc ggcgtaagca tcggcgtcgg caacttcccc accgacggcg agacctccga
     8581 cgatctgctc atggcggccg accggaacat gcgtcgcgac aaaagcgcac gcaaacccaa
     8641 ccctgagctg tccctcgcgg ccgggcttgc gcccgctgac gcattgcgtt aattccccac
     8701 caacagcgaa aacagtgtcg cccgccggca agtgtccggc gggcttttcc ttgtcttttt
     8761 gcgcccgaac ccgcaactcg cgtataatcc ggcctcgaag gcaaatcatc cagacagctt
     8821 tattccgaac tcgcgccatc gagtcgaggc gcagcagcgt ctgcgcggcg gccggggacg
     8881 cgctttccac gggtgagaag aaaatgagtg agctgaaagt aattccgatg aagccgcgca
     8941 ctagctctgc cgaagccggc gcgcagggcg cacaggtgcg tgaggcggct ggaactgacg
     9001 ccccggtcgt gcccatcgaa gtgcatgagc gccgccggca ggagcagcaa caccgtgtcg
     9061 aggagatcga ccgcgagact ttattgcgga tgctctatca gatgatcctc gtcaggcgtt
     9121 tcgaggagaa gtcggcggaa gcctatacgc tcggcaagat tgggggcttc tgccatctct
     9181 acatcgggca ggaagccgta gccgtcggcg tcatctcggc cctgcgtaaa gacgactacg
     9241 tcctgacgag ctaccgcgac cacggccacg ccctgattct cggcacctca cccgacgagg
     9301 tgatggccga actctacggc aaggccggcg gctgctcggc cggcaagggc ggctcgatgc
     9361 atctcttcga cgccgacacc aactttctcg gcggccacgc catcgtcggc ggacaaatcc
     9421 cgctcgccac cggcgtcgcc ttcgcctcca agtatcgcgg caccgaccag ctcgcggtct
     9481 gcttcttcgg cgaggccgcc gtcaatcagg gcgccttcca cgagtcgctg aacatggcgc
     9541 agctttggaa gttaccctgc atctacatct gcgagaacaa ccagtacggc atgggcacgg
     9601 ccgtcaagcg cgcgatgtca ctgcacgaag tggtggagaa ggcccccgcc tacgacatgg
     9661 cctcggagtt cgttgacggc atggacgtgc tcgcggttcg ggaggcgaca caacgcgccg
     9721 tcgagcgtgc gcgcgccgac tcgctgccga cgctgctcga agtaggcacc tatcgcttca
     9781 tgggacactc gatgtccgac cccggcaact accgcacgcg cgctgagatc gaaaagcatc
     9841 aggagcgaga ccccatcaag cttttcgccg acagcctgaa gaagaagcaa gtcatcggcg
     9901 acgctgacct aagcgaaatc gagaagcgcg tcaaggaaga ggtcgagcgc gccctcaagt
     9961 tcgccgacga aagccccgag ccggcgcccg aggagctttt tacaaacatc tacgccaatc
    10021 cgttaggaaa gtgatgagtg atgagtgatg agtgataaga aagaaaagaa gctgttagct
    10081 tttagctgtt ggctgttagc taatcgctaa aagctaacag cttcttatca ctcatcactc
    10141 atcacttatc actgcttttg gaggtttccg aatggccatc atcacgatgc gcgaggcgct
    10201 caatcaggcg ttggcggagg agatgcgccg cgacgagggc gtctttctga tgggcgaaga
    10261 ggtcgcggcc tatcagggcg cgtacaaggt ctcgaagggg ttgctcgaag agttcggcga
    10321 gaagcgcgtg atcgactcgc cgattaccga gctgggcttc gccggtctcg gtgtcggcgc
    10381 cgcgatggtc ggcctgcgcc ccgtcatcga gttcatgacc ttcaacttct ccatcctcgc
    10441 caccgaccag atcatcaact ccgccgccaa gatgctctac atgtcgggcg gccagttcaa
    10501 aatccccatc gtcttccgcg gccccaatgg ctccgcctat caagtctcct cgcagcactc
    10561 gcagtccctt gagtcgtggt tcgcgcactt ccccggcctc aaggtcgtga tgccctcgac
    10621 gcccgccgac gcgaagggct tgctcaagag cgccatccgc gacgatgacc ccgtcatctt
    10681 catggagcag gagcgcatgt acggcctcaa gggcgaggtg cctgacggtg acgactttac
    10741 aatcccgctc ggcgtggccg acgtgaagcg cgagggcaaa gactgcacca tcgtcgcgcg
    10801 ctcgctcacg gtgcccatcg ccctgaaggc ggcggaggag atggagaagc agggcgtgtc
    10861 ctgcgaggtc atcgacccgc gcacgatccg gccgctcgac acggagacca tcatccgttc
    10921 ggtgaagaag acgaaccgcg tcgtcatcgt cgaagagtcg cacccttttt gcggcgtagg
    10981 cgcagagttg agcgcgcaga tcatggagaa cgccttcgac catctcgacg cccccgtcaa
    11041 gcgtctctcc ggcgttgacg caccgatgcc ttacgcgaag aaccttgaac gtctcgcgct
    11101 gccggacgtg ccacgcctcg tcgccgccgt gcgcgaggtc tgttacatgg attaaaggag
    11161 aagaagtcag gagtcaggag tcaggagtca gaatatgaag agcaaaactt gtcttcattc
    11221 tgactcctga ctcctgactc ctgactccat gatgttatgg ctacacaggt tgtaatgccc
    11281 aagctctcgc cgacgatgga agaggggcag ctcgcgcgtt ggctcaagaa ggagggcgac
    11341 aaggtctccg tcggcgagcc aatcgcggag atcgacacgg acaaggccac gatggaggcg
    11401 caggcgctga cgaccggcgt gctgcgaaaa atcctcgtgc ccgccggcga gaccgttccc
    11461 ctcggcgcga tcatcgcggt catcgccgag gccgacgaag atatctccga catcctctct
    11521 aaagccggct cggcccaagc gacgaccgcg cggaaggaag acacacagga gccggtgaag
    11581 acgggcggcg acggttcaga gacggagtcg tccggcgtcg tgcccggcgg cgagcgcacg
    11641 ccggcgaccg ttgaggggca gccaatcgcg ggtgatgtcg gtggtgcgcc cgccgctaaa
    11701 gattcggacg actcagacga cacgctggaa aagtcacag
//
LOCUS       DASVES010000173         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_579004, whole genome shotgun sequence.
ACCESSION   DASVES010000173 DASVES010000000
VERSION     DASVES010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579004"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF240_06995"
     CDS             <1..268
                     /locus_tag="VF240_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175011.1"
                     /translation="QRYNFQFGGQTFWEIKKGKITGMLRDVAYQARTTDFWNSLDALG
                     GPATYQLGGSFNDGKGEPGQSNAVSHGCPIARFRNINVLNTRRS"
     gene            409..1773
                     /locus_tag="VF240_07000"
     CDS             409..1773
                     /locus_tag="VF240_07000"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7175012.1"
                     /translation="MLLTEKEARAATQKMLSFVTADDAVVAVGSEIYSHLRFAANSFQ
                     TSGRRENVSATVAVWKDAGAGAGRKRGAASANALDEASLREAVAEAERVAALSPPDRE
                     YLPSLGPQTYRPTQGFAEATANISPAARAKFIGDIITEAERAKVIAAGFHQARGAAGA
                     TATKNGNFAYERTSNVSLSMTTRTPEGDSSGYFLRSHFDVARLDTLRIARESIRRALE
                     GRNARALEPGAYTVILEPQAVADLLNFGFAFDARNAEEGRSAYSAPDGKTRRGQKIFD
                     ERLNIYSDPWNPELPGSQAAQAGLPAEKIFLVRGGVLESLVYSRFWAQKRNEKPTPGP
                     VNIIMESAAAAASVEEMISSTERGLLVSRFWYIRGTDPRRAASTGLTRDGVWLIEGGK
                     IQHAVKNFRFNQSMTEMLAPGNVEMIGKPERVGSSEGGGGGASLMPALKIKKFNFTSQ
                     SEAV"
     gene            1984..2517
                     /locus_tag="VF240_07005"
     CDS             1984..2517
                     /locus_tag="VF240_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEX7175013.1"
                     /translation="MSKQSTPEVSPMPINIGIGEADRREIADGLSRLLADTYTLYLKT
                     HNFHWNVTGPMFQTLHLMFEQQYNELWTAGDLIAERIRALGFFSPGTYKEFARLASVK
                     EDEGVPAATGMVRQLVEGHETTARTARSVIEVAEHAKDAPTADLLTERLQVHEKTAWM
                     LRSLLADEGTTANAQSA"
     gene            complement(2873..>4159)
                     /locus_tag="VF240_07010"
     CDS             complement(2873..>4159)
                     /locus_tag="VF240_07010"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX7175014.1"
                     /translation="FVELLSGTRGTKSKKALVVEALRNATPLEAKYLVKLLTGDLRIG
                     LKEGLVEDSVARAFERPLAEVAHANMLTGDIGEAAVLARRGEHAGLTMRLFHPIKFML
                     ATAAEDLADVARQMPEEFYVEDKFDGIRAQAHAERGRVAIYTRTLDEVTHRFPELVAP
                     LAALPPGVLIDGEIVPVRGPEILPFQELQKRLGRKTVGEELLASVPVAFIAYDLLYAA
                     GRVLIGEPYSERRRALESLVGGDGAGALRLSDAKLFGDVVLLDDEFDAARSRGNEGLM
                     IKDPRSSYKPGKRGREWLKLKRALATLDVVVTSVEVGHGKRRHMLSDYTFAVRRSEED
                     AELLGVGKAYSGLTDAEIVELTEWFRAHTVQEFAHGRVRVVEPKIVLEVTFDRVQESK
                     RHKSGYALRFPRIVRLRDDKTPDEIDTLETVRKLAG"
BASE COUNT          768 a         1431 c         1283 g          677 t
ORIGIN      
        1 gcagcgatac aacttccagt tcggcgggca gactttctgg gaaatcaaaa agggcaagat
       61 cacggggatg ctgcgcgacg tggcctatca ggcgcgcacc accgacttct ggaactcgct
      121 cgacgccctc ggcggcccgg ctacctatca gctcggcggc agcttcaacg acggcaaggg
      181 cgagccgggt cagtccaacg ccgtctcgca cggctgcccc atcgcgcgct tccgcaacat
      241 caacgtgctg aacacgcgac ggtcgtagga gagctaatag cggttagctg ttagctgtta
      301 gctcacggca gacgccgccg ggcgcgccgc ggtcgagaca caacatactg gttcgctctc
      361 ttaaagctaa tagctaaccg ctaatagcta accgctgagg tgctaaacat gctactcacc
      421 gagaaggaag cccgcgccgc cacccaaaag atgctctcgt tcgtcacggc cgacgacgcc
      481 gtcgtcgccg tcgggagcga aatctattcg cacctgcgct tcgccgccaa ctctttccag
      541 acgagcggcc ggcgcgagaa cgtgagcgcc acggtcgccg tctggaagga cgccggcgcc
      601 ggggcgggcc gcaagcgcgg cgcggcctcc gccaacgcat tggacgaggc atccttgcgc
      661 gaagccgtcg ccgaagccga gcgcgtggcc gctctctcgc cgcccgaccg cgagtatctg
      721 ccgagtctgg ggccgcaaac ctaccgaccc acgcaaggct tcgccgaggc gaccgccaac
      781 atctcgccgg ccgcgcgcgc gaagttcatc ggcgacatca tcaccgaggc cgagcgcgcg
      841 aaggtcatcg ccgccggctt ccatcaggcg cgcggcgcgg ccggcgccac ggcgacgaag
      901 aacggcaact tcgcctacga gcgcacgagc aacgtcagcc tctcgatgac cacccgcacg
      961 cccgagggcg acagctccgg ctacttcctc cgcagccact tcgacgtggc ccgtctcgac
     1021 accttacgca tcgcccgcga gtcgatccgc cgcgccctcg aaggccgcaa cgcgcgcgcc
     1081 ctcgaacccg gcgcttacac cgtcatcctc gaaccgcagg ccgtggccga cctcctcaac
     1141 ttcggcttcg ccttcgacgc ccgcaacgcc gaggagggcc gcagcgccta ctccgcgccc
     1201 gacggcaaga cgcgccgcgg ccagaagatt ttcgacgagc gcctgaacat ctacagcgat
     1261 ccctggaacc ccgaactccc cggctcgcag gccgcgcagg ccggactccc cgccgagaaa
     1321 atcttcctcg tcaggggcgg cgttctggag agtctggtct actcgcgctt ctgggcgcag
     1381 aagcgaaatg agaagccgac gcccggcccc gtcaacatca tcatggagtc ggccgccgcg
     1441 gcggcgagcg tggaggagat gatcagctcg accgagcgcg gcctgctcgt cagccgcttc
     1501 tggtacatcc gcggcacaga cccgcggcgg gcggcctcca cggggctgac gcgcgacggc
     1561 gtctggctca tcgaaggcgg gaagattcaa cacgccgtca aaaacttccg tttcaaccag
     1621 agcatgaccg agatgctcgc gcccggcaac gtcgagatga tcggcaagcc cgagcgcgtc
     1681 ggctccagcg agggcggcgg cggcggcgct tcgctcatgc ccgcgctcaa aatcaaaaag
     1741 ttcaacttca cctcgcagtc cgaagccgtc tgaaaagtgt caggtttaaa gtggaagggg
     1801 taagggaaag atgaacttct tttcccttac cccttccctt ttcatccctc gccacaaatc
     1861 gaacaactca tgtgacgaaa tggcgaaact atgcccacat ttttagtgct taatctatct
     1921 tcggcgtttt gtcggcccgc gggcctggcg gggcgccgga agaaagagag aagcagcatt
     1981 catatgtcaa aacagtccac gcccgaagta tctccgatgc ccatcaacat cggcatcggg
     2041 gaggcagacc ggcgcgagat cgccgacggc ctctcgcgcc ttttggccga cacctacacg
     2101 ctctacctca agacacacaa cttccactgg aacgtcacgg ggccgatgtt ccagacgctc
     2161 cacctgatgt tcgagcagca gtacaacgag ctgtggacgg ccggcgacct catcgccgag
     2221 cgcatccgcg cgctcgggtt cttctcgccc ggcacgtata aagagttcgc gcgtctcgcc
     2281 tccgtcaagg aagacgaggg agtgccggcg gcaaccggga tggtgcgcca gctcgtcgag
     2341 ggccacgaga cgacggcgcg caccgcgcgc tccgtcatcg aggtggccga gcacgccaag
     2401 gacgcgccaa cggccgacct cctcaccgag cgcctgcaag tccacgagaa gaccgcgtgg
     2461 atgctccgca gcctcctcgc cgacgagggc acaaccgcca acgcgcagag cgcgtaaggc
     2521 aaaagtaaaa aggcaatggg caaaagttga agaagccgag tcgcaggcgc cgacgagccg
     2581 ccggaggcgg cggcagcagt gttgcccacg gcgtgagccg tgggaacagt ccgcgataaa
     2641 ataaggaagc cccggagggg cggaagaacc tcaacgctgc acgtcgcggc gcgtgaaagt
     2701 tctttcgccc ctccggggct tccgattttt tattcgcact ctaccccccg tctcacgact
     2761 ggggctacac tgctgtcgcc gctccgcggc tcatggacgc cttcggcggc gacgcctgcg
     2821 gcttggcctt ttaacttttg ccttttgcct ttttactttt gccttcttta gtttatcccg
     2881 cgagcttgcg aacggtctcc agcgtgtcga tctcgtcggg cgtcttgtcg tcgcgcagtc
     2941 gcacgatgcg cgggaagcgc agggcgtagc cgctcttgtg tcgctttgat tcttgcacgc
     3001 ggtcgaaggt gacttccaga actatcttcg gctcgacgac cctcacgcgc ccgtgcgcga
     3061 actcttgaac ggtgtgcgcg cggaaccact cggtcagctc cacgatctcc gcatccgtca
     3121 gccctgaata agccttgcca acgcccagca gctccgcgtc ttcttcagag cggcggacgg
     3181 cgaaggtgta gtccgagagc atgtgccggc gcttgccgtg gccgacttcg acggaggtca
     3241 cgaccacgtc gagcgtggcg agagcgcgct tgagcttcag ccactcgcgc ccgcgcttgc
     3301 ccggcttgta ggacgagcgc gggtctttaa tcatcaaccc ctcgttgccg cgagagcgcg
     3361 ccgcgtcgaa ctcgtcgtcg aggagcacca catcgccgaa cagcttcgcg tcggacaggc
     3421 gcaacgcgcc cgcgccgtct cccccgacga gactttcgag cgcgcgccgt ctctcactat
     3481 aaggctcgcc gatgaggacg cgccccgccg cgtacaggag atcgtaggcg atgaaggcga
     3541 cgggcacgga ggcgagcagt tcctcgccca cggtcttgcg gcccagacgc ttctgcaatt
     3601 cctgaaaggg cagaatctca ggcccgcgga cggggacgat ctcgccgtcg atgaggacgc
     3661 ccggcgggag cgcggcgagc ggcgcgacga gttcggggaa gcggtgcgtc acctcgtcga
     3721 gcgtgcgcgt gtagatggcg acgcgcccac gttcggcgtg cgcctgcgcg cggatgccgt
     3781 cgaacttgtc ttcgacgtag aactcttcgg gcatctgccg ggccacgtcc gcgaggtctt
     3841 cggccgccgt cgcgagcata aacttgatgg ggtggaacag acgcatcgtc agccccgcgt
     3901 gctcgccccg gcgcgcgagc acggccgcct cgccgatgtc gcccgtgagc atgttggcgt
     3961 gagcgacctc cgcgagcggg cgctcgaagg cgcgcgccac ggagtcttcc accagcccct
     4021 ccttcagccc gatgcggagg tcgccggtga gcagcttgac gagatacttg gcttcgagcg
     4081 gcgtcgcgtt tcgcagcgcc tcgacgacca acgctttctt gctcttcgtg ccgcgcgttc
     4141 cgctcagtag ctcgacgaa
//
LOCUS       DASVES010000174         9384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1368387, whole genome shotgun sequence.
ACCESSION   DASVES010000174 DASVES010000000
VERSION     DASVES010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368387"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="VF240_07015"
     CDS             complement(<1..1532)
                     /locus_tag="VF240_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7175015.1"
                     /translation="MKDEKAAAKVEQFMSGLKSSSLIPHPSSLAAWLERALTLSIFLL
                     VVCAPLTIAGTQGAWLLAGLLWLIRLGVRPKTGPRRTPLDYWLLGFFVLTFFSALLSY
                     DPDVSIGKLRAASLFTIVYVVAENVTSPRVLRALVIALVASCAASNVYTFGVYVIGRG
                     VRIDALAADSPLHTLGLRPGDTVLEVNGQRLRGPEPLARALDSRAAPDGGALTVRLLV
                     HRLEAYPLLDYPRLPLPEGRSAAERLGLVSWSRGRDVRAAGTYGHYTTYAEVLQLVAS
                     LVAGLLVALRRKASWRGVLLAAALAGMCGALILTVTRASWLGLLLSALVIASAGAGRR
                     TLLIAAGLALPLILGALFVLQQKRQVGFIDPQEGSTAWRLTVWSEAVGVLVSEPRHLL
                     VGVGRDTLKRHWREWGMFDGGRLPWGHLHSTPLQIAFERGLPALLVWLGLLAAYARML
                     WRLARRGGGGGEESLGWVERGLALGALGGTVGFFTSGLVHYNLGDSEVVMVFYLVMGL
                     ALA"
     gene            complement(1544..3109)
                     /locus_tag="VF240_07020"
     CDS             complement(1544..3109)
                     /locus_tag="VF240_07020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7175016.1"
                     /translation="MRAERLNKVRVEVSEVRAAARAPGWVMPAVKAALVAADAVLAVA
                     CFVAAYVMREGGAVLLGFSLREGFAWAPEFEPYGSLLWLVLVIRLIALGYYDLYRLRG
                     EFSYVDEAARVFKALSVASLLVVAAAFLYRGGMEFRAFSYSRAVFVLDYALALAAYGS
                     LRLVVRGVQSAVRRREVNLIPTLVVGRGREAALCVREMRERRELGYRVIGVVENGEAS
                     APGVPTEFEGVPVVGGVGQLHELILETGANEVIITDPSVSGDLLFDVMMRVGRRRGVE
                     FRIAPSLFNCLPRKTEIDQIGALPVITLFREPLGGAARLVKRASDVLIASLALFVLAP
                     FWLLIAVLIKLDSRGPVVYRQERVGMDGRIFLFLKFRTMLTDADDREHREFQRRYIEG
                     HPETNLGDAERPVFKLYDDPRVTRTGRVLRRLSLDEMPQLLNVLRGDMSVVGPRPPIP
                     YEVEAYELWHRKRLDMKPGLTGLWQVSGRNRLSFDEMVRLDLFYIENWSLWLDLRIIL
                     KTLPVVLRGDDAY"
     gene            complement(3236..4258)
                     /locus_tag="VF240_07025"
     CDS             complement(3236..4258)
                     /locus_tag="VF240_07025"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX7175017.1"
                     /translation="MLIWIDLANSPHVPFFRALAVEFRRAGHEVETTARDFAQTVELA
                     ERAGFAPAIVGGHGGRELAGKAGNLLGRAWGLARWARGRSIDLAVSHNSYSQIVAARL
                     TALRSVTLMDYEHQPANHLAFRLASRVIVPAAFPESALRRFGARPRKVRRYDGTKEDV
                     YLADFAPDPTFAETLRALGIAEGDTLVVARPPARDALYHRFENELFDELIEHCAAARP
                     GVKVLMLARTNGQREELRARFQSESFVWPREAVDGANLVASADLVVSAGGTMNREAAA
                     LGVPAATVYAGRWAAIDEQLVREGRLRKISTREDVCALRVERKPAANPRRATHVRAQV
                     AERILE"
     gene            complement(4312..5538)
                     /locus_tag="VF240_07030"
     CDS             complement(4312..5538)
                     /locus_tag="VF240_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175018.1"
                     /translation="MQEEFERVYEKLSAWCRERDYAGHDPFDALNSRLFRSTPLLGRS
                     RLARLAWTQALKRSPVNLRRPALVPAERNSKGTALFALAALARHRANRDDAAAEREAR
                     ALLDDLLEARVRAPSGAAWGYNFDWQGRAFYAPRGTPTVVPTAFAIRALVEGSQLFDE
                     RADGGAQGDAEGARDKYLEAARGACEFILRDLHRGDGGGDDEVCFSYTPLDRTRVFNA
                     SLLAGEALAAVGARTGERALLETALAAARYVVRRQRADGSWAYGEADFQSWADNFHTA
                     FILTSLARICRAQPAARAELGDAVRRGYRFWRENFFLADGWPKYYHRSAYPADAHSAG
                     AAVVALTELGEDDPDALEFAAVVALWAVRHLFDERGFFYYQRRRRYTVRTPYMRWSQA
                     WMAYALARLLEVSSKQ"
     gene            complement(5610..6656)
                     /locus_tag="VF240_07035"
     CDS             complement(5610..6656)
                     /locus_tag="VF240_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7175019.1"
                     /translation="MKIYDEEETVLGGRVGAAEEPTLGANGGGGDAGAPEISVFLPVL
                     NEEPNLRPLHIKLDESLARLGRTAEIIYVDDGSTDGSLEVLRELAQRDPRVRVIALRR
                     NYGQTAAMAAGIDAARGAVLIPMDADLQNDPADIIRLLDKLDEGFDVVSGWRKERQDK
                     MVSRKIPSMIANRIISFIGGVKLHDYGCSLKAYRREFLKEVKLYGEMHRFIPIYASWV
                     GARVTEIPVQHHARTMGQSKYGLSRTFKVMLDLMTIKFMASYHTKPIYVFGLFGFSSF
                     LISIVAGVWAVMRKLGGESFIRNPLLVVAIVMFAVGVQFILMGLLAEMLVRTYHESQA
                     KTVYVVRERVGFTD"
     gene            complement(6907..7872)
                     /locus_tag="VF240_07040"
     CDS             complement(6907..7872)
                     /locus_tag="VF240_07040"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7175020.1"
                     /translation="MRKILVTGGAGFIGSHLVERLLGEGGWRVTVVDDFNDFYDPAVK
                     RANVRAHVGREDFRLAEADIRDHAALERVFAEEGFDCVVHLAARAGVRPSLAEPVLYA
                     ETNISGTLNLLELARASGVRQFVFGSSSSVYGENEKTPFAEDDPIFKPISPYAATKAA
                     GELLCHTYAHLWDLRCVCLRFFTVYGARQRPDLAIHKFARLISEGRPIPVFGDGTTRR
                     DYTYIDDIINGVRAGIDYDASRYEVVNLGESRTVELRELITLLEKELGQSAVIDRQPL
                     QPGDVPQTFADITKARRLLGYDPRTQIEEGVRRFVEWFKGQRAEG"
     gene            complement(7954..8847)
                     /locus_tag="VF240_07045"
     CDS             complement(7954..8847)
                     /locus_tag="VF240_07045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7175021.1"
                     /translation="MADATHNLKERVRAFWQANPCGTKFADAEVGTREFFRRVEEHRY
                     RTEWHIPGAAGFESARSLRVLEVGCGLGTDGARFARAGAVYTGVDLTEAAVSLARRRF
                     ELEGLAGEFRVSDAENLDFADESFDLVYSHGVLHHTPDTARAVREIRRVLRPGGRAVV
                     MLYHRDSYNYRINIRLLRRLGARLLRSEAGVGLAHRLTGEPVESLREHVAGMKTDAES
                     YRAPGEFLSRNTDGAGNPLSRVYSRREARELFRDFAGVRLAAHFLNKRWLPLVGPALP
                     RSLEARLAARWGWHLWIYGEK"
     gene            complement(8996..>9384)
                     /locus_tag="VF240_07050"
     CDS             complement(8996..>9384)
                     /locus_tag="VF240_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175022.1"
                     /translation="GRPWADKTFDLRVDDHAEPLRELRRLIGLQRAYNHMNAGDAAVE
                     RKDNAGALREYSAAQKLVPDNLEMAYWHAVALVGMNRTDEALPIFRRVFAADPNWRTM
                     TPRLVKSGILPNDPKLIERITREGQK"
BASE COUNT         1654 a         3110 c         3186 g         1434 t
ORIGIN      
        1 gccagcgcga ggcccatgac gaggtagaag accatgacca cctcggagtc gccgaggttg
       61 tagtgaacca gcccgctcgt gaagaagccg accgtgccgc cgagcgcccc caacgcgagt
      121 ccgcgttcaa cccaaccgag cgactcttcg ccaccgccgc cgccgcgtcg cgccagacgc
      181 cagagcatgc gcgcgtaggc ggcgagtagg ccgagccaga cgagcagcgc cggcagcccg
      241 cgctcgaaag ctatctggag cggcgtcgaa tgcaggtggc cccagggcag gcgcccgccg
      301 tcgaacatcc cccactctct ccagtgccgc ttgagcgtgt ccctgccgac gccgacgagc
      361 aggtgacgcg gctcgctcac gagcacgccc accgcctcgc tccacaccgt cagacgccac
      421 gcggtcgatc cctcctgcgg gtcgatgaag ccgacctgcc gcttctgttg caggacaaag
      481 agcgcgccca agatcagggg gagcgcgagg ccggcggcga tgagcaacgt gcgccggccc
      541 gcgccggccg aggcgatgac gagggccgac aacagcagcc ccagccacga ggcgcgcgtc
      601 accgtcagga tcagcgcgcc gcacatcccg gcgagcgcgg cggccaacaa cacgccccgc
      661 cagctcgcct tgcggcgcag tgccaccagc agtcccgcca ccagcgacgc gacgagttgg
      721 agaacttcgg cgtaggtcgt gtaatgaccg tatgtgcccg cggcccgcac gtcgcgtccg
      781 cgcgaccagc tcacgaggcc gaggcgctcg gctgccgaac ggccttcggg cagcggcagg
      841 cgcgggtagt cgagcagcgg gtacgcctcc agtcggtgga cgagcagccg cacggtgagc
      901 gcgcccccgt ccggcgccgc acgcgagtcg agcgcgcgcg ccaggggctc ggggccgcgc
      961 agacgctgac cgttgacttc gagcaccgtg tcgccggggc gcaggccgag cgtgtgaagc
     1021 gggctgtcgg ccgccagcgc gtcgatacgc accccgcgcc cgatgacgta gacgccgaac
     1081 gtgtagacgt tgctcgcggc gcacgacgcg acgagcgcga tgacgagcgc gcgcagcacg
     1141 cgcggcgacg tgacgttctc ggccacgacg tagacgatgg tgaagagact cgcggcgcga
     1201 agtttaccga tggacacgtc ggggtcgtag gagaggagcg cggagaagaa tgtgaggacg
     1261 aagaagccga gcagccagta gtcgagcggc gtgcggcgcg gccccgtttt ggggcgcacg
     1321 cccagtctga tgagccacag cagtcccgcc agcagccacg ccccctgcgt ccccgcgatg
     1381 gtgagcggcg cgcacaccac cagcaggaag atgctgagcg tgagggcgcg ttcgagccac
     1441 gcggcgaggg atgagggatg agggatgagg gatgaagatt tcaggccaga catgaactgt
     1501 tcgactttcg ccgccgcctt ttcatccttc atccctcatc ccttcagtag gcgtcgtcgc
     1561 cgcgcagcac gacggggagg gtcttgagga tgatacgcag gtcgagccag agcgaccagt
     1621 tctcgatgta gaagaggtcg aggcgaacca tctcgtcgaa ggagagacgg ttgcggcccg
     1681 agacctgcca gagtcccgtc agccccggct tcatgtcgag ccgcttgcgg tgccagagtt
     1741 cataagcctc cacctcgtaa ggaatcggcg ggcgcgggcc gacgacgctc atgtcgccgc
     1801 gcaggacgtt taagagctgc ggcatctcgt ccagactcag gcggcgcagg acgcgccccg
     1861 tgcgcgtcac gcgcgggtcg tcgtagagct tgaagacggg gcgctcggcg tcgccgaggt
     1921 tggtctcggg gtggccctcg atgtagcggc gttggaactc gcggtgctcg cggtcgtcag
     1981 cgtccgtgag catcgtgcgg aacttgagga agaggaagat gcggccgtcc atcccgacgc
     2041 gctcctgccg gtagacgacg gggccgcgcg agtcgagctt gatgagcacg gcgatgagca
     2101 gccagaacgg cgcgaggacg aacagcgcaa gcgacgcgat taaaacatcc gaggcgcgct
     2161 tgacgagacg cgcggcgccg ccgagcggct cgcggaagag tgtgataacg gggagcgcac
     2221 cgatctggtc gatctcggtc ttgcgcggca gacagttgaa gaggctcggc gcgatgcgga
     2281 actcgacgcc gcgccgccgc cccacgcgca tcatcacgtc gaagagcagg tcgcccgaca
     2341 ccgacgggtc ggtgatgatg acttcgttag cgcccgtctc cagaatcaac tcgtgcagtt
     2401 ggcctacgcc gccgactacg gggacgccct cgaactccgt gggcacgccc ggcgcggacg
     2461 cctccccgtt ctccaccacg ccgatgacgc ggtagcccag ctcccgacgc tcgcgcatct
     2521 cgcgcacgca cagcgcggct tcgcgcccgc gccccaccac cagcgtcggg atcaggttga
     2581 cctcgcgccg gcgcaccgcg ctttgcacgc cgcgcacgac gaggcgcagc gagccgtaag
     2641 cggcgagcgc gagcgcgtag tctaagacga agacggcgcg cgagtaggag aaggcgcgaa
     2701 actccattcc gccgcgatag aggaaggccg ccgccacgac gaggagcgag gcgaccgaca
     2761 gagccttgaa gacgcgcgcc gcctcgtcca cgtaggagaa ctcgccgcgc agacggtaga
     2821 ggtcgtagta accaagcgcg atgagtcgga tgaccagcac gagccacagc agcgagccgt
     2881 atggctcgaa ctccggcgcc cacgcgaacc cctcgcgcag gctgaagccc aacagcaccg
     2941 ccccgccctc gcgcatgacg taagccgcga cgaagcaggc gacggccagc accgcgtccg
     3001 ccgcgacgag cgccgccttc accgcgggca tcacccaccc cggcgcccgc gctgccgccc
     3061 gcacctcgct cacctccaca cgcactttgt tcagacgctc agctctcatc tcaagaaggt
     3121 gataagtgat gagtgataag tgataagaaa gaaaggaagc tgttagctgt tagctgttgg
     3181 ctgttagcta atcgcttaga gctaacagct cttatcactt atcactcatc acttatcact
     3241 ccaatatcct ttccgcgacc tgcgcgcgca cgtgggtggc gcggcggggg ttggcggccg
     3301 gcttgcgctc gacgcgcagc gcgcacacgt cttcgcgcgt ggagatttta cggagccggc
     3361 cctcgcgcac gagttgttcg tcgatggccg cccagcgccc ggcgtagacg gtcgcggcgg
     3421 gcacgcccag cgccgcggcc tcccggttca tcgtcccgcc cgcgctcacc acgaggtcgg
     3481 ccgatgcgac gaggttcgcc ccgtccacgg cctcgcgcgg ccagacgaag ctctcggatt
     3541 ggaagcgcgc gcgcaactcc tcgcgctgcc cgttggtgcg cgcaagcatc agcaccttga
     3601 cgccgggccg cgccgccgcg cagtgctcga tcagttcgtc gaacaactcg ttctcgaaac
     3661 ggtgatagag ggcgtcgcgc gccggcggtc gggcgacgac gagcgtgtcg ccttccgcga
     3721 taccgagcgc gcgcagagtc tccgcgaaag tcgggtcggg cgcgaagtcc gcgagataca
     3781 cgtcctcttt cgtgccgtcg tagcgccgaa ctttccgggg ccgcgcgccg aagcgccgaa
     3841 gcgcactttc ggggaacgcc gcggggacga tgacgcgcga ggcgagcctg aaggcaagat
     3901 gattggccgg ctggtgctcg taatccatca gcgtcacgga gcgaagcgcc gtcagccgcg
     3961 ccgccactat ctgcgagtaa gagttgtggc tgacggccag atcgattgag cgcccacgcg
     4021 cccagcgcgc gagaccccac gcgcgcccca gaaggttccc cgccttcccc gccaactctc
     4081 gcccgccgtg gcccccgaca atagcgggcg cgaaacccgc gcgctcggcc agctcgacgg
     4141 tctgcgcgaa gtcgcgcgcc gtcgtctcga cctcatgccc cgcccgccga aattcaacgg
     4201 cgagcgcgcg gaagaaaggc acgtgcggcg agttcgccag atcgatccaa ataagcatgt
     4261 gagcagtgac agtgagcagt gagcagaaga aaagttttta ctgcttactg cttactgctt
     4321 actgctcact tccaaaagtc tcgccagcgc ataagccatc caggcttgcg accagcgcat
     4381 gtagggcgtc cgcacggtgt agcgtcggcg acgctggtag tagaagaagc cgcgctcgtc
     4441 gaagaggtgg cggacggccc agagcgcgac gacggcggcg aactctaagg cgtcggggtc
     4501 gtcctcgccg agttcggtga gcgcgacgac ggccgcgccc gcggagtgtg catcagcggg
     4561 gtacgccgag cgatggtagt atttcggcca accgtccgca aggaagaagt tttcgcgcca
     4621 gaatcggtaa ccgcgccgga cggcgtcgcc gagttcggcg cgcgccgccg gctgagcgcg
     4681 gcagatgcgc gcgagcgagg tgaggatgaa ggccgtgtgg aagttgtcgg cccacgactg
     4741 gaagtcggcc tcgccgtagg cccacgagcc gtcggcgcgc tgtcgccgga cgacgtagcg
     4801 cgccgccgcg agggccgtct ccaaaagcgc ccgctcgcct gtgcgcgccc cgaccgcggc
     4861 caacgcctcg ccggccagca gcgaggcgtt gaagacgcgc gtccggtcga gcggcgtgta
     4921 gctgaagcag acctcgtcgt cgccgccccc gtcgccgcga tgcagatcgc gcaggatgaa
     4981 ctcacacgcg ccgcgcgcgg cctccaaata cttgtctcgt gcgccctcgg catcgccttg
     5041 cgcgcctccg tccgcgcgct cgtcgaagag ttgcgacccc tcgacgagcg cgcggatggc
     5101 gaaggcggtt ggcacaaccg tgggcgtgcc acgcggcgcg tagaaggcgc ggccctgcca
     5161 gtcgaagttg tagccccacg ccgcgccgga cggggcgcgc acgcgggctt cgagtaagtc
     5221 gtcgagcagt gcgcgcgctt ctcgctccgc tgccgcgtcg tcgcggttcg cgcggtggcg
     5281 ggcgagcgcg gcgagggcga agagggccgt gcccttcgag tttctttcgg cggggacgag
     5341 cgcggggcgg cgcaagttga cgggcgagcg tttgagggct tgcgtccacg cgaggcgggc
     5401 gagacgcgag cggccgagca gcggcgtgga acggaagagc cggctgttga gcgcgtcgaa
     5461 cgggtcgtgc ccggcgtagt cgcgctcgcg gcaccacgcc gaaagttttt cgtagacgcg
     5521 ctcgaactcc tcctgcatcg cccgccaaga tacaacagac gggggagcgc gaacgaggcg
     5581 cgaagcggcg gcagtcaata acccgtccgt cagtccgtga acccgacgcg ctcgcgcacg
     5641 acgtagacgg tcttggcctg cgactcgtga taggtgcgaa cgagcatctc ggcgagcagc
     5701 cccatgagga tgaactgcac gccgacggcg aacatcacga tggcgacgac caaaagcggg
     5761 ttgcggatga acgactcgcc gcccagctta cgcatcacgg cccagacgcc ggccactatc
     5821 gagatgagga acgacgagaa gccgaagagg ccgaagacgt agatcggctt cgtgtggtag
     5881 gaggccatga acttgatggt catcaggtcg agcatcacct tgaaggtgcg cgacagacca
     5941 tacttcgact ggcccatcgt ccgcgcgtgg tgttggacgg gaatctccgt gacgcgcgcg
     6001 ccgacccaac tggcgtagat cgggatgaag cggtgcatct cgccgtagag ttttacttcc
     6061 ttcagaaact cgcggcggta agccttcagc gagcagccgt agtcgtggag cttgacgccg
     6121 ccgatgaagg agatgatgcg gttggcgatc atcgacggaa tcttgcgcga gaccatcttg
     6181 tcctgccgct ccttgcgcca gcccgagacc acgtcgaagc cctcgtcgag cttgtcgagc
     6241 aggcggatga tgtcggcggg gtcgttctgg agatcggcgt ccatggggat gagcacggcc
     6301 ccgcgcgcgg cgtcgatgcc ggcggccatc gcggccgtct gcccgtagtt gcgccgcagg
     6361 gcgatgacgc gcacgcgcgg gtcgcgctgc gccagttcgc gcagcacttc gaggctgccg
     6421 tcggtcgagc cgtcatcgac gtagataatc tcggcggtgc ggccgaggcg cgcgagcgac
     6481 tcgtcgagct tgatgtgcag aggccgcagg ttcggctcct cgttcaagac cggcaggaag
     6541 acggaaatct ccggcgctcc ggcgtcgccg ccgcccccgt ttgcgccgag cgtcggctcc
     6601 tcggccgcgc cgacgcgccc gccgagcacg gtctcttcct cgtcgtaaat cttcattctc
     6661 actacctctt ccgcaccatc ataaacgatg gagacgggcg gttaaacctg taggggcagg
     6721 gcaagtccgc ggcccgctca actatcaggg caattcaaac atggatggac aggatgggca
     6781 gggtaagaga ttccagaggt caaatctgaa atctccaatc agctctttcc tcatcctgac
     6841 tatcggtgtc catccatgtt gattttttct ttagcaacgg gccgcggact tgccctgccc
     6901 ctacaatcat ccttctgccc gttgtccctt gaaccactcg acgaagcggc ggacgccctc
     6961 ttcgatttgc gtgcgcgggt cgtagccgag caggcgacgc gccttcgtga tgtcggcgaa
     7021 tgtctggggc acgtcgccgg gctggagcgg ctggcggtcg atgacggcac tttgtcccaa
     7081 ctctttctca aggagtgtga tcaactcgcg aagctcaacc gtgcgcgact cgccgaggtt
     7141 gacgacctcg tagcggctcg cgtcgtagtc tatccctgcg cgcacgccgt tgatgatgtc
     7201 gtcgatgtag gtgtagtcgc gccgcgtcgt gccgtcgccg aagacgggga tgggccttcc
     7261 ctcactgatg agccgcgcga acttgtggat ggcgaggtcg gggcgctggc gcgcgccgta
     7321 gacggtgaag aagcgaagac agacgcagcg caagtcccac aggtgcgcgt aggtgtggca
     7381 gagcagctcg cccgccgcct tcgtcgccgc gtaggggctg atgggtttga agatggggtc
     7441 gtcctcggca aacggcgtct tctcgttctc gccgtagacg gaggacgatg agccgaagac
     7501 gaactgtcgg acgcctgagg cgcgcgccag ctccagcagg ttgagcgtcc ccgagatgtt
     7561 cgtctcggcg tagagcaccg gctcggcgag cgatgggcgg acgccggcgc gcgcggccag
     7621 atgaacgaca cagtcgaagc cctcttccgc gaagacgcgt tcgagcgcgg cgtggtcgcg
     7681 gatgtcggcc tcggccagcc ggaagtcctc acgcccaacg tgcgcccgga cgttcgcgcg
     7741 cttcacggcc gggtcgtaga agtcgttaaa atcatcaacg acggtgacgc gccagcctcc
     7801 ctcccccagc aggcgctcga cgaggtgtga accgatgaaa cccgctccac ctgtcaccag
     7861 aatctttcgc atacctttag tagtgataag tgatgagtga taagtgataa gaaagagatt
     7921 gtcttcttct tatcacttat cactcatcac ttatcacttt tccccgtata tccacaggtg
     7981 ccagccccag cgggcggcga gccgcgcttc gagagagcgc gggagcgcgg ggccgacgag
     8041 gggcagccaa cgcttattca aaaagtgggc cgcgaggcgg acgccggcga agtcgcggaa
     8101 aagctcgcgc gcctcgcgcc ttgagtagac gcgcgacaga gggttgccgg ccccgtccgt
     8161 gttccggctc aggaactcgc cgggcgcgcg gtagctctcc gcatcagtct tcatccccgc
     8221 gacgtgctcc cgaagcgact cgaccggctc gcccgtcaga cggtgcgcca gcccgacgcc
     8281 ggcctcgcta cggaggaggc gcgcgccgag gcggcgcagg agtcggatgt tgatacggta
     8341 gttgtaggag tcgcggtggt agagcatcac gacggcgcgc ccgccgggcc gcagcacgcg
     8401 tcgaatctcc cggacggcgc gcgccgtgtc cggcgtgtga tgcaggacgc cgtgcgagta
     8461 gacgagatcg aagctctcgt cggcgaagtc cagattctcc gcgtccgaga cgcgaaactc
     8521 gccggccaat ccctccaact caaagcgacg ccgcgcgagc gagacggccg cctccgtcag
     8581 atcgacgccc gtgtagacgg cgcccgcgcg ggcgaaacgc gccccgtccg tgccgagacc
     8641 gcagccgact tcgagcacgc gcagactccg cgcgctctcg aacccggccg cgccggggat
     8701 gtgccactcc gtgcggtagc ggtgctcctc gacgcgccga aagaactcgc gcgtgcccac
     8761 ctcggcgtcg gcgaacttcg tcccacacgg gttcgcctgc cagaaggccc gcaccctctc
     8821 cttcaaatta tgcgtggcgt ctgccatcac ttacgcgggg gacgcgcatg ctctgtatag
     8881 ctcaaaaact cagcgtcacc ctaaacgcag aaaagtaaca tgcccgccga ggcgggacaa
     8941 ggaacgccgc cggccggggc gcagcccttt ctcacccacc tttcctcttc cctttttact
     9001 tttgcccttc tctcgtgatg cgctcgatga gtttcgggtc gttggggagg atgccggatt
     9061 tgacgaggcg gggcgtcatc gtgcgccagt tgggatcggc ggcgaagacg cgtcggaaga
     9121 tggggagggc ttcgtcggtg cggttcatgc cgacgagggc gacggcgtgc cagtaggcca
     9181 tctccaggtt gtcggggacg agtttttgcg ccgccgagta ttcgcgcagc gcgccggcgt
     9241 tgtccttgcg ctcgacggcg gcgtcgcccg cgttcatgtg gttgtaggcg cgctgcaagc
     9301 cgatgaggcg gcgcagctcg cgcaggggtt ccgcgtggtc gtccacgcgc aggtcgaagg
     9361 tcttgtcggc ccacgggcgg cccg
//
LOCUS       DASVES010000175         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_209913, whole genome shotgun sequence.
ACCESSION   DASVES010000175 DASVES010000000
VERSION     DASVES010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209913"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VF240_07055"
     CDS             <1..1193
                     /locus_tag="VF240_07055"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX7175023.1"
                     /translation="VWKYSGHEDTFSDPLVDCRNCNSRFRADKLVEEVGAADFAAVKC
                     PKCGGANWTEPRPFNLMFRTTIGAVAEEDDPNALAYLRPETAQGIFINFLNVQTTMRR
                     KIPFGIAQIGKSFRNEVTPGNFIFRTREFEQMEMEYFVRPGEDERVHQEWIDYCFEWF
                     KTLGISPDNLRFYEQPKEELAHYAKRTVDIHYRFFPEREEEERQWDELMGIANRTDFD
                     LKAHSKKPEDAEGKRVNPDSTEDLSYFDEATKERFYPYVIEPAAGVNRTVLAVLMDAY
                     TEKVNDKGEKRVILGLHPTLAPIKVAVLPLAKNKSEIVSLAKQIKRTLQPSMRAVYDD
                     TGGIGKLYARQDEVGTPFCVTVDHQSLEDEAVTVRDRDTWEQERVKVAELEGHLKRRL
                     GIKC"
     gene            1326..1793
                     /locus_tag="VF240_07060"
     CDS             1326..1793
                     /locus_tag="VF240_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175024.1"
                     /translation="MAIVKKRNEQTETRLLLESDEPVELPPAEVSEGAVVLQFETFRQ
                     YIPELLEALRAGAVAEPVPVMLDGSTFVVHGATRSFWVKLWHAARTELARVERVQVIS
                     CLPSTRGLQVIVDDPDDFLEDDDDEDDYDDEEGELEAADGDLEDGDEGKEELR"
     gene            1911..3320
                     /locus_tag="VF240_07065"
     CDS             1911..3320
                     /locus_tag="VF240_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX7175025.1"
                     /translation="MPPPIPEKILKLARAVREAGGRALMVGGCVRDRLMGREAKDWDV
                     EVYGVEPSLLRAILDRAGRVNVVGEAFTVYKIGQNLDVSLPRRERKTGRGHRSYFIEG
                     DPAMSFEDAARRRDFTVNAILEDPLTEEIIDPFDGRADLGRKLLRAVSRDTFAEDSLR
                     VLRAAQFAARFEFRIDPETIALCRTIDLTDLPPERVWGELEKLLLGARRPSVGFEWMV
                     KLGVVEKIFPEIQALIGVPQEEAWHPEGSCEIHTWLVCDRARELIDDLDYSRRVTVML
                     AALCHDFGKPATTAFIEGRIRSRGHEEAGVAPTESFLDRLKIYTLDGYDVRAQVVALV
                     RDHLKPGELYRRRAEIGDGAFRRLARRCELDLLYRVARADSLGRNAPWVPPEKWYTAE
                     AQEWFIARARELQVQQRPPAPVLMGRHLIEMGLAPSPLFKRILDAVYEMQLDGRVRDV
                     EEARAAASRLVAEAGEETS"
     gene            3543..4898
                     /locus_tag="VF240_07070"
     CDS             3543..4898
                     /locus_tag="VF240_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175026.1"
                     /translation="MLKKLVLVAFACLAAWPLLVSESKTPPSPAPEPPPAATAFAFPD
                     ARDNPPASWTGPVFRLSQNYPTTMPSPETYPWKAIDFKTKPMEYLRAVLAYCLEGNIT
                     SSGDFDINQNTTRKWYHAPWMHPNREFIHGMTNERHSRPGELHPLQTSPWLNWAVGMY
                     NAPGGYVIGRVWANPTSPDPSLARFPDGTVGFKLLFTQAPATQVPYLANSFEWLTDIN
                     QVSKTGKPPVQMRLLQIDVAVRDTRANSTTGWVFGTFTYDAQEPGSTPWQRLVPIGLM
                     WGNDPTRLFNGQPLTQTKINPDLRMQQHLGYGVGGHKRLNGPVDNPVSSCLSCHSTAA
                     VSLNPPRPTISGVTPPNPTNQQIRTYFRNISSGTPFSPGYTSLDYSLQLQVGIALFVR
                     SGGFQPPGPTPPGPMTATTMASSARRSAAAGAMIKPMERDGTPPPTTTRARRPRRRRA
                     R"
BASE COUNT          935 a         1759 c         1511 g          792 t
ORIGIN      
        1 cggtctggaa gtactccggc cacgaggaca ctttcagcga ccctcttgtg gactgccgca
       61 actgcaacag ccgttttcgc gccgacaagt tggtggaaga ggttggcgcg gccgacttcg
      121 ctgccgtcaa gtgcccgaag tgcggtgggg cgaactggac ggagccgcgg ccgttcaacc
      181 tcatgttccg tacgaccatc ggcgcggtgg ctgaggagga cgacccgaac gctttagctt
      241 acctgcggcc cgagacagca cagggcatct tcatcaactt cctgaacgtg cagacgacga
      301 tgcggcgcaa gattccgttc ggcatcgcgc agatcggcaa gtcgttccgc aacgaggtga
      361 cgcccggcaa cttcatcttc cgcacacgcg agttcgagca gatggagatg gaatacttcg
      421 tccgccccgg cgaggacgaa cgcgtccatc aggagtggat tgattactgc ttcgagtggt
      481 tcaagacgct cggcattagt cccgacaacc tgcgcttcta cgagcagccc aaagaggaac
      541 tggcgcacta cgccaagcgc accgttgaca tccactatcg cttcttcccc gagcgtgagg
      601 aagaggagcg acagtgggac gagctgatgg gcatcgccaa ccgcacggac ttcgacctca
      661 aggcgcactc caaaaaaccc gaggacgccg aagggaagcg cgttaacccg gactcgaccg
      721 aagacctctc atacttcgac gaggccacga aggagcgatt ctatccttac gttatcgagc
      781 cggcggcggg agtcaaccgc acggtgctcg ccgtcctgat ggacgcctac acggagaagg
      841 tgaacgacaa aggggagaag cgcgtcatcc tcggcctgca cccgacgctc gcgccgatca
      901 aagtggcggt gctgccgctc gccaagaaca agtcggaaat cgtctcgctg gcgaagcaga
      961 tcaagcgcac cttgcagccc tcgatgcgcg ccgtctacga cgacacgggc ggcatcggca
     1021 agctctacgc ccgccaggac gaggtcggca cgcccttctg cgtcaccgtc gatcaccagt
     1081 cgctcgaaga cgaggccgtc accgtgcgcg accgcgacac gtgggagcag gagcgcgtga
     1141 aggtggcgga actggagggg cacctcaagc gacgcctcgg catcaagtgc tgaaagccgt
     1201 gaatcgtgaa tcgtcaatcg tcaatcggta aggctaagtt tccccgcgtt gcgattaagc
     1261 attggcgttg agtatcgaat ccatcacgat tcacgatttt cgattgacga ttcacgaacc
     1321 aacatatggc gatagtcaaa aagagaaacg aacagaccga gacgcgcctg ctgctcgaat
     1381 cggacgaacc cgtggaattg ccgcctgcgg aagtgagcga gggcgcggtc gtcctccagt
     1441 tcgagacgtt ccgacagtac atcccggagc tgctcgaagc cctgcgcgcc ggcgccgtgg
     1501 ccgagccggt tccggtgatg ctcgacggct cgaccttcgt cgtccacggc gcgacgcgta
     1561 gcttctgggt caagctctgg cacgcggccc ggacggagct tgcgcgcgtc gagcgggttc
     1621 aggtcatcag ttgtctgccc tcgacgcgcg ggctacaggt catcgttgac gacccggatg
     1681 attttctgga ggacgatgac gacgaagacg attacgacga cgaggagggc gaacttgagg
     1741 cggccgatgg cgacctcgaa gacggcgatg aaggcaagga agagttgcgc tgaacgcttc
     1801 ctcgccgctc atgcgtgaac tctgcgttct ctgcgcctct gcggtgagtc cgagtcgccc
     1861 cacactcaag ccacagacca tcccccgagc ggaggcccac aaacttaccc gtgccccctc
     1921 ccatccccga aaaaatcctg aagctcgccc gcgccgtgcg tgaggccggc gggcgcgcgc
     1981 tcatggtggg cggctgcgtg cgcgaccgcc tgatggggcg cgaggcgaaa gactgggacg
     2041 ttgaagtcta cggcgtcgag ccttcgcttt tgcgcgccat cctcgaccgc gcgggtcgcg
     2101 tcaacgtcgt cggcgaggcg ttcaccgtct acaaaatcgg gcagaacctc gacgtaagcc
     2161 tcccccgccg cgagcgcaag accggacgcg ggcatcgctc ctacttcatc gagggcgacc
     2221 ccgcgatgtc ttttgaagac gccgcccgcc gccgcgactt caccgtcaac gccatcctcg
     2281 aagaccccct gaccgaagag atcatcgacc ccttcgacgg ccgcgccgac ttggggcgca
     2341 aactcctgcg cgccgtctcg cgcgacacct tcgccgaaga ctccctgcgc gtgctgcgcg
     2401 ccgcgcagtt cgccgcccgc ttcgagttcc gtatcgaccc cgagacaatc gcgctctgcc
     2461 gcacaatcga cctcaccgac cttccgcccg agcgcgtctg gggcgagttg gagaagctgc
     2521 tcctcggcgc gcgccgcccc tcggtcggct tcgagtggat ggtcaaactc ggcgtcgtcg
     2581 aaaaaatctt ccccgagata caggcgctca tcggcgtgcc gcaggaagag gcgtggcatc
     2641 ccgagggaag ttgtgaaatc catacctggc tcgtgtgcga ccgcgcgcgc gaactcatcg
     2701 acgacctcga ctactcgcgc cgggtgacgg tgatgctcgc cgcgctctgc cacgacttcg
     2761 gcaagccggc gacgacggcg ttcatcgagg ggcgcatccg ctcgcgcggg cacgaggagg
     2821 ccggcgtcgc gccgactgag agcttcctcg accgcctgaa gatttacacg ctcgacggct
     2881 acgacgtgcg cgcgcaagtg gtcgccctcg tgcgcgacca tctcaagccc ggcgagctgt
     2941 accgccggcg cgccgagatc ggcgacggcg ccttccgccg cctcgcccgc cgttgcgaac
     3001 tcgacctcct ataccgcgtc gcgcgggccg actccctcgg ccgcaacgcc ccgtgggtgc
     3061 cgccggagaa gtggtacacg gcggaagcgc aggagtggtt catcgcgcgc gcgcgcgagc
     3121 tacaggtgca gcaaaggccg cccgcgcccg tcctcatggg ccgccacctc atcgagatgg
     3181 gacttgcgcc ctcgccgctc ttcaagcgga ttctcgacgc cgtctacgag atgcagctcg
     3241 acgggcgcgt gcgcgacgtg gaagaggcgc gcgcggccgc cagccgtctc gtcgccgagg
     3301 cgggcgaaga aacatcttga cttcgcaccc gaaaggaatt attcagggcg tgcgacacga
     3361 accctctcgc cggccgcgcc gccccgccgc gacgacgcgc ccgaccactc gcgggacgat
     3421 ttgaaaccgc cctcgcactc aaccgccgca taatccccgc ccgccagcgg ggctgcaccc
     3481 cgtcgccagc agacgccttc aacgctcttt ccaactcgac ctcaacctct cggaggaccc
     3541 acatgctgaa gaaattagtc ctcgtcgcct tcgcctgcct cgccgcctgg cccctgctcg
     3601 tctccgaatc gaagacgccc ccgtcgcccg cgcctgagcc gccgcccgcc gccacagcgt
     3661 tcgccttccc ggacgcgcgc gacaaccctc cggcaagttg gacggggccg gtcttcagac
     3721 tcagtcagaa ctacccgacc acgatgccgt cgcctgagac gtacccctgg aaggcaatcg
     3781 atttcaagac caagccgatg gagtacctcc gcgccgtgct cgcctactgc ctcgaaggca
     3841 acatcacgtc cagcggcgac ttcgacatca accagaacac cacgcgcaag tggtatcacg
     3901 ccccgtggat gcacccgaac cgcgagttca tccacggcat gaccaatgag cgccattcgc
     3961 gcccgggcga gctgcatccg ttgcagacat caccgtggct gaactgggcc gtcgggatgt
     4021 acaacgcgcc gggcggctac gtcatcggcc gcgtgtgggc caaccctact tctcccgacc
     4081 catcgctcgc ccgcttcccc gacggcacgg tcggcttcaa gctgctcttc acgcaggcgc
     4141 cggccaccca ggtgccctat ctcgcgaact ccttcgagtg gctgacagac atcaaccagg
     4201 tctccaagac cggcaagccg cccgtccaga tgcgcctgct ccagatcgac gtggccgtgc
     4261 gcgacacgcg cgccaacagc acgaccgggt gggtcttcgg caccttcacc tacgacgcgc
     4321 aggagcccgg ctcgacgccg tggcagcgcc tcgtccccat tggcctgatg tggggcaacg
     4381 acccgacgcg tctcttcaac gggcagccgc tcacgcagac caaaatcaac cccgacctgc
     4441 ggatgcaaca gcacctcggt tacggggtcg ggggacacaa gcgtctgaat ggccccgtgg
     4501 acaacccggt ctcgtcgtgt ctgtcgtgcc attcgaccgc cgcggtctcg ctcaacccgc
     4561 cgcgcccgac catctccggc gtcacgccgc cgaacccgac gaaccagcag atcagaacct
     4621 acttccgcaa catcagttcc ggcacaccct tctcccccgg ttacacgtcg ctcgactact
     4681 cgctgcaact acaggtcggc atcgccctct ttgtccgctc gggcggtttc cagccgccgg
     4741 ggccgacgcc ccccggcccg atgacggcca cgacgatggc gtcgtccgcg cgacgcagcg
     4801 cggcggcggg cgccatgatc aagcccatgg agcgtgacgg aacaccgccg ccgaccacga
     4861 cgcgcgcccg caggccgcga cgcagaaggg caagatagac gagagtgacg cgggcatcgc
     4921 gctttcggtg cgccggccgg ggttttcgcc tgttcgcgtc gttgatgtaa gatacatttt
     4981 tagcctcgcc cgatccc
//
LOCUS       DASVES010000176        27256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_585740, whole genome shotgun sequence.
ACCESSION   DASVES010000176 DASVES010000000
VERSION     DASVES010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 27256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585740"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VF240_07075"
     CDS             <1..664
                     /locus_tag="VF240_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175027.1"
                     /translation="DNAPQTNAPASVVVPLSSHRRGASPWRLPRWAQGAAVAAASVLL
                     VVGLYGVFRKQSPPPAQVAQTDVQKSNAVVEQKQEVRSAQIADEGDTTDAAGDRSADV
                     VEPERREPEVFTPRRNNRAGGARPNFQMAKFGNGGARRPAATGAGADATEEITTNFFP
                     LMNGGQLTPGDAGHVIRVEVPRTALASFGLPVNADRAGGRVKADVLMGEDGMARAIRF
                     VQ"
     gene            764..1900
                     /locus_tag="VF240_07080"
     CDS             764..1900
                     /locus_tag="VF240_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175028.1"
                     /translation="MKKSRLTTLALAGLMATSSGAGVVAQQQQVKEKQRIQEVIVGQD
                     GVVITPAPGGFGTGVGMGQGAGVSVGGGDQNFVFVSSEMAIDGKVVKGAPYSADAVTE
                     TTQTLADGNRIVRKNTAQVYRDSEGRTRREHTISVVGPFATEGDPPRTIFIHDPVAST
                     NYILDPRSRTARKVNAVRTFERMIDSGTRFKTEVLAQAEAKAAGVGETSPAANGQEPK
                     QRVRSSGYVYTTPSGVGHHPGGPEVMFYKHDGAEPKIEKLASQTVEGVLAEGQRITHT
                     IPAGSIGNEQPINIVDERWYSPELQTVVMTRHSDPRSGETVFRLTNINRSEPARTLFE
                     VPSDYTVREVPSGVNTFRRKRAEGGAQKENNFQFHYNRNEQQQQ"
     gene            complement(2024..2146)
                     /locus_tag="VF240_07085"
     CDS             complement(2024..2146)
                     /locus_tag="VF240_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175029.1"
                     /translation="MQGPMRGILAGIAAWKLGGGCISTILVFIVVFYLLGYVNC"
     gene            2290..4113
                     /locus_tag="VF240_07090"
     CDS             2290..4113
                     /locus_tag="VF240_07090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7175030.1"
                     /translation="MPPLDFDPLPDPMDNSPTNHPAPPPLLRRRWAEIVCAAALFVFA
                     ANSFLVISRKNFTTDEAFMIPAGYYHLTARDFRPVNEHPPLVKLLSAVPLLFLDPAAA
                     PHERLEARFYSDPNNLYWTFWRDNRERIDELSFWARVPAVLLTVLLGALVFCYTRRLF
                     GARAAAFAVLLFTLEPTVLAHGRVVQTDIPASLGLLLFTFALYDYLRGPSARRAAWVG
                     AAAGLAVVTKFSMVVLAPVIVVAFVALIWRAPRRATGRGAVVAQFAVCALAALLTINA
                     AYLFQHRPPDEGDTAIILSRIVSDPSSEGLRQWLAGAYAVVVKIIPADFLEGVGWQLT
                     HASEGHDAGLLGRYSKHGWWYYFPVAFALKTTLPFLLLSIVGIVWAVRGFMKEGGGRW
                     LVVLVPFVLYTALVMSSSINIGVRYYLPAFPFLCIAAGVFLDRLLRRRRPESEPRPRR
                     ALLATVVVVAAFAWMGVEAARAFPDYMTYTNQLAAGRPHWWYLSDSNVEWGDDVAELA
                     RYLKARGETQVRASLLNYIVLEDYGVGYRWAYLPPGAPTPADVRYVAIGASYLNGSIN
                     PGSLEGRLLSDEERLNLFDDYRRRTPEKIFGRSIYLYRVRD"
     gene            4530..5279
                     /locus_tag="VF240_07095"
     CDS             4530..5279
                     /locus_tag="VF240_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX7175031.1"
                     /translation="MNHSSTLVILPTYNERENVGDLVAQILAYAPDADVLLVDDNSPD
                     GTAPYAEELFAGEPRFSVLRREGPRGLGRSYVDGYRTALERGYARLVQMDADFSHDPS
                     RLPDILTAARTADVVVGSRYCPGGGIGNWPLRRRLLSRFANSYVSAITGVKVGDATSG
                     YRCYNRPALEHVLGNRIVAEGYAFLVESIYRISEAGLKISEVPIVFVDRREGKSKISR
                     AVIFESVLMPWKLRLRPRLPAPADPALARDN"
     gene            5480..6415
                     /locus_tag="VF240_07100"
     CDS             5480..6415
                     /locus_tag="VF240_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7175032.1"
                     /translation="MKRILLAAALLAAASAPACTPTTGPANNSNAPANSNAAATPTPA
                     PTVAAADHIAREKQVMDAIKRKDWDAFAAHLADDQLYVSTDGVHDKAATLASIKKLDI
                     TEYTSADARVVQIDRDLVVVTHTTTAKGTFDGKPIPAGSQRESTAWANRGGRWLAVYH
                     QDSEVMTPPTPTPGAPTPAATAVATPVAAASATPAATPANSTELEQQIWDTLKRKDWD
                     AFAAHLAADAVEVGATGVYDKAGTVNAVKQVDFTGATLSDFKEVKIDADATLLIYTAK
                     GPRAVFGPNGERSATIHANRSGRWLAVFHQATSIK"
     gene            complement(6512..8914)
                     /locus_tag="VF240_07105"
     CDS             complement(6512..8914)
                     /locus_tag="VF240_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7175033.1"
                     /translation="MSERSASLAEVLDEMTVEGAPELTEHLAGGALRCYACGHRCLIG
                     EGRRGICKVRFNEQGRLLVPANYVAALQCDPTEKKPFFHVLPGSDTLTFGMLGCDLHC
                     GYCFTGDTVVVTDRGPMTFTEAFESAVRVERRPDADIAYPKDLRAVAVSGTPRRVRGV
                     FRHRYRGKLAAVRPYYLPELRCTPDHRLYATDDVTRAPELTRAEQLTTRHYLAIPRRY
                     SFSTPQTINVAAELGGHRISYRVPWGLTAEVRRSITVATERGETSRAIGARLGKDASY
                     IRHVRSKLARGRAQDVRTSGPRIEGDTLRFPNEHRPGIPLSVPLDENMARLLGLYCAE
                     GSVTSSKTRPNSHALNFSFSHGEGRLVEEVAGLLEKCLGVRAARVRRTTTLAATCNKS
                     SAALLFQSLAGSRAAGKRVPAAVFNAHRHIARAFLDAYVEGDGHRYANGKVSITTVSR
                     SLAYGVAWLALKLGHLPSVYDAEQSERGFVQGRAVRRSPHQYTVVWYEGANVGRKAVE
                     AGNFYLVPLRGVSSVDYEGDVYNMEVEEEHNYLAGFFAASNCQNWITSQALRDDAAGA
                     APKMVTAERLVAMAHAYGARVVGSSYNEPLITAEWAVEVFRRAKPEGFRTAFISNGNA
                     TPQVLDYLRPWTDCYKIDLKSMSDRNYRQLGGVVENILDTVRMVRERGFWEEIVTLVI
                     PGFNDSDDEIRRAADFIASVSPDIPWHVTAFHQDYRMTENANTTADQLVRACETGRAA
                     GLRYVYAGNLPGRVGRWENTHCHNCDEPLVERYGYVIRKMKIGPDGKCPACGTTIPGV
                     WA"
     gene            9032..9703
                     /locus_tag="VF240_07110"
     CDS             9032..9703
                     /locus_tag="VF240_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7175034.1"
                     /translation="MIIEQITVTSFQQHARIVGCDETRRCICIDPGDDTEEIVAAIDR
                     HGFELQAIALTHAHLDHVGGVAALKRRRPRAEVCIHAGDEPLYLGLREQPLWLGIPRS
                     EWARYGFDYEAPPPVERRWEDGEEYPVGRLRFRVIHCPGHTPGHVVLFEPARRVVFVG
                     DCLFAGSIGRTDLPGGSTEQLMDSLLNKIIPLGDDVTVYSGHGPETTIGRERQTNPFL
                     TGRYF"
     gene            complement(9802..10086)
                     /locus_tag="VF240_07115"
     CDS             complement(9802..10086)
                     /locus_tag="VF240_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175035.1"
                     /translation="MADENIQQEAVKSQPDVEKSQPDLEKSQLDAAHATQLSNEIRQG
                     TGEFDARFLLWRTFCAENNVPVETMPGDLKGEVKDIWDKLKNEKLKVSDK"
     gene            complement(10189..11133)
                     /locus_tag="VF240_07120"
     CDS             complement(10189..11133)
                     /locus_tag="VF240_07120"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX7175036.1"
                     /translation="MTRHAGELLMGNDEHDKEALALDALDALDGEELRELQAHLASCT
                     ECRRERDALRDAASMLAYATPHVAPGGHVRARLLERVRELGPANAEAVAPASPAAQTR
                     AEVSDAAQGDARVLEFAARPPSHSPVIPKWFALAASLVIAVMAVALAVLWRQNNDLRG
                     ELARSGAELNAERSRDAQQQEALERGREELQWARELAELIASPEARMASLAGTKDAPP
                     AAHATVAYDRATSRAVLISSGLPPAPAGKAYQVWYIDGGKPPVPGGTFKPNVDSRGFL
                     SDRIPADGLAAQTFAVTLEPEGGVPAPTGKMYLLSAAS"
     gene            complement(11179..11763)
                     /locus_tag="VF240_07125"
     CDS             complement(11179..11763)
                     /locus_tag="VF240_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7175037.1"
                     /translation="MIALPEQDKVDAELIHAVARGDERALASLYDHYSHILFGLLLRI
                     LKDRPEAEDVLQEVFLQIWQRASSFDAERGRPFTWLVTLARSRAIDRLRSVRSRERAA
                     LASTAEAPEPVADASDEAVRSEQGEAVRGALASIPEEQRRALLLAYFEGLSQTEIAAR
                     LDQPLGTVKTRMRSGMLKMRELLRGQVEGAGAAM"
     gene            complement(12012..13106)
                     /locus_tag="VF240_07130"
     CDS             complement(12012..13106)
                     /locus_tag="VF240_07130"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX7175038.1"
                     /translation="MATQLKRPAVPGSIEELLGDGARALLDFNAPKISKDLLQVPGPD
                     FVDRVWAQSDRNPSVLRSLQSLFDAGRLGGTGYLSILPVDQGIEHSAGASFAPNPAYF
                     DPDNIVKLAIEGGCNAVASTFGVLGAVARKYAHKIPFIVKVNHNELLTYPNKFDQVMF
                     GAVEQARDMGAAAVGATIYFGSEESTRQLSEIAESFAIAHELGMATILWCYLRNPKFK
                     GKEGDMHTAADLTGQANHLGVTIQADIIKQKLPERNGGYNMLNVESSYGKTNPKIYTE
                     LTSDNPIDLVRYQVANCYMGRCGLINSGGESKGASDMAEAVRTAVINKRGGGMGLISG
                     RKAFQRPMNEGVQLLNAIQDVYLDAEITVA"
     gene            complement(13362..14561)
                     /locus_tag="VF240_07135"
     CDS             complement(13362..14561)
                     /locus_tag="VF240_07135"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX7175039.1"
                     /translation="MSQSLHPSSLIPPPSSSPVLPGATVGVLGSGQLGRMFALAARRM
                     GYRVHTFSPHANTPTGQVADCEYRNSYDDLDAVREFARGVACITFEFENVPAAAVEAA
                     AQLAPVRPSARVLHTTQNRLREKTFLSTSGFPVPTFRHVTSLEDLRAALSEVGCPAVL
                     KTAGFGYDGKGQTRLARPEDAADAFAGLDGQEGILEAFVDFECELSVVAARSGSGEFR
                     HYGAVENTHRRHILDLSVAPALCVPAAASRAAVEMARGVLDALDVVGVMCVEFFLTRD
                     HTLLINELAPRPHNSGHFTVDACLTNQFEQQLRAVCQLPLGSTTQLRPAAMVNLLGDL
                     WAAGEPDWTAALAHPDIKLHLYGKRDARPGRKMGHLTALADTPAEAARLALASRAALT
                     RGVMSDK"
     gene            complement(14676..15230)
                     /gene="purE"
                     /locus_tag="VF240_07140"
     CDS             complement(14676..15230)
                     /gene="purE"
                     /locus_tag="VF240_07140"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7175040.1"
                     /translation="MGEVNTISDGETQGSNPSEAEVSPLVAVIMGSTSDWETMRHAAD
                     VLRQFDVPHECRVVSAHRTPQLLAEFAARAEERALEVIIAGAGGAAHLPGMVAAQTTV
                     PVLGVPVESRCLRGVDSLLSIVQMPAGVPVGTLAIGQAGATNAALLAVAILANTRPPL
                     RERLRQFRREQTEKVLAATLEEQG"
     gene            complement(15282..15806)
                     /locus_tag="VF240_07145"
     CDS             complement(15282..15806)
                     /locus_tag="VF240_07145"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7175041.1"
                     /translation="MTAYTAAPPGADEYPADFEGYVSLVPEGDVLQTLARQIDDTLDI
                     LRAVSEERAASAYAPGKWTMKQLAGHLVDSERVFAYRALCIARGDTASLPGMDQNVYV
                     EHGNFDARTLASLADELARVRATTLDLLSHLDEPAWLRRGTANDNEMSVRALAHIIAG
                     HEVHHVKVLRERYL"
     gene            complement(15851..16024)
                     /locus_tag="VF240_07150"
     CDS             complement(15851..16024)
                     /locus_tag="VF240_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175042.1"
                     /translation="MAENEGQAKAPDASEEESAVELGPIEKLEEQTPTGSIVPGAQKP
                     SSEVHNEVNLNTE"
     gene            complement(16175..17644)
                     /locus_tag="VF240_07155"
     CDS             complement(16175..17644)
                     /locus_tag="VF240_07155"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX7175043.1"
                     /translation="MAEKTPITVARGDGIGPEIMEATLHILKEAGAALDIEEIEIGEK
                     VYLAGNPAGVAPDAFDSLRRTRVFLKAPITTPQGGGYKSLNVTVRKTFGLYANVRPCV
                     SYHPFIETKHPVMDVVIVRENEEDVYGGIEYRQTRQVTQCLKLISRPGTEKIARYAFE
                     YARRNNRKKVTCFTKDNIMKITDGLFHKVFEEVAAQYPDIENEHWIVDIGAAKLADTP
                     ENFDVVVLPNLYGDVLSDVAAQIAGSVGLAGSANIGESIAMFEAIHGSAPRRAGQNLA
                     NPSGLFLGAVMMLVHINQTEAAERAHNAWLRTIEEGIHTYDIYKEGVSREKVGTREFA
                     EAVAKRMGQKPETLKAVSYRAADAKDLAGGPQSSTVGETVKNENKDLVGVDVYFEWTN
                     GSPDELGEQMQNLSGEGLRVSSIANRGVKVWPGGAAETFCSDHWRCRFVSDAERGATV
                     THAQISALLRRAADAGLDFIQTENLYNFDGSAGYSHAQE"
     gene            18109..19284
                     /locus_tag="VF240_07160"
     CDS             18109..19284
                     /locus_tag="VF240_07160"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7175044.1"
                     /translation="MNPPTEFELLLCLARVSLRASDEARARAILGRGGFDWELFHSLA
                     WRHGLMPLAFRHLLERFAAHVPEAHLARAREDFRHNSARNLLLASELRGVLDALAAEG
                     VAAVAYKGPALAVQVYGDLKLRSFVDLDVLVRRADAERAGRVLASVGYRPHLSLTPAQ
                     EAMLARSECDRVYFKEGRGVMLELHWAVAPPYFSLALETEDVLDGATRVELCGREVVT
                     PSAEMLLLLLCVNGAKDLWRALEPLCAVAELSRARGGVDWPRVVRLARRAGALRMLFV
                     GLLLARDLLDAPLPAEVARLADSDRAARRLAGEASRRLAERETRAPGLAEETRFRVAA
                     RERLRERARYCALRLLTPTYRDCSFELPPALRFVYYVLRPLRLLGGVLKRPASKPVL"
     gene            19363..20646
                     /locus_tag="VF240_07165"
     CDS             19363..20646
                     /locus_tag="VF240_07165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175045.1"
                     /translation="MIQGRDIIYISSIEWSFLWQGHQEIALRLAAAGNRVLYVENTGV
                     RSPGLRDAGRVVARARRWARAAPSQGARELAPNIHVSSPVVLPPFGGAARRALNRRFF
                     LPSLLAAARRLGMRNPLVWTYLPNDTTLDLIHMLGAERAGLVYYCVADFSQLTPHPRR
                     LEAVEREIVETADVVFTNCSELKMKFAPWNPHTHVYPFGVNLDAFPFEAQAPSGLNGS
                     NGNGAAPPERGSAPTSPAFVASLPRPVIGYVGGMHRHVDFGLIREMALRRPEWSWVCV
                     GALQAATGDLAGLPNVHLTGQRPHAELADYVRGFDVCVVPYVDSRYTATVVPTKINEY
                     LAVGKPVVSTALPPVREFNEEHRGVLLTAEAEAESFLAAVERALELPRDEETRRRRRR
                     VAEQADWGARFTQMCDLVEARLDARGAWQSPAASL"
     gene            20727..21650
                     /locus_tag="VF240_07170"
     CDS             20727..21650
                     /locus_tag="VF240_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175046.1"
                     /translation="MERCDTKVKGSRAARALRLMMAALVLGVAFGAAGARAQQPSPTP
                     QQTTTPPTPAPADDAKQPTAAADPREPAAAAKPGFTLRVTKQGPLRVYKLVAKNAKVS
                     DIAADLSRQIGAPVQLSPLMQRQRVTVEFSNLDLEAALRFLAPVPLVDYVAGGADAAS
                     GQPKPLAVYLYAVNEAPPALNETVKSSSEAFLIEGHTEEGTEEYEKQKKEEPLEITFA
                     NNRLSVRAEKQPLSIVLIKVAGALGIPFELNHNVTEVVNLNFQQYPVDQAMRSISTSV
                     HFFYRADLSTSEKYPIRLSLSASAARSGDRN"
     gene            21653..21940
                     /locus_tag="VF240_07175"
     CDS             21653..21940
                     /locus_tag="VF240_07175"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX7175047.1"
                     /translation="MQGQTMSDTPAPHDHVVATEFDGGEGVLVDLNSKRYYQLNETAM
                     LVWRCLERGLSSAEIVGAMTARYDVSADHAAGSLSNVLRSLEAHKLVGLRP"
     gene            22253..22426
                     /locus_tag="VF240_07180"
     CDS             22253..22426
                     /locus_tag="VF240_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175048.1"
                     /translation="MTELSSAQPNDGETVSAKKPFVEPVVSVPQDVLEATAFFQGSAG
                     VGGGGADGTDGLD"
     gene            22470..23501
                     /locus_tag="VF240_07185"
     CDS             22470..23501
                     /locus_tag="VF240_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175049.1"
                     /translation="MSIPEVGPAPTHGSEPASERFYEIAGRRLSIEAEAGSAGNLASA
                     YVRGFHLTQRPRPDADQLDLRFRVVADQPPLPAGLRQFEIPEGVCHADQRRHYLDVGG
                     SRVVVEERAARRVTVWFGEAARAPGSFALITTMAYAMPAALRRVGLYDLHATGMVEPE
                     SGHGFLFPGSSGSGKTSLAVRLGAAGWQYMSDDLMLLCGERPVVEMWPLRRHFQADAL
                     SLANCPLPRLEEAIGMVIATDPNKRRLNPTILFPGRFVATATPRVLCFPVVTGEGESR
                     VEALGQAEAMMRLVSMCPWSSYDLAAARDHLRLLGRLVRQCRLFTLRAGRDIFDSPER
                     AGRLLASLA"
     gene            23515..24615
                     /locus_tag="VF240_07190"
     CDS             23515..24615
                     /locus_tag="VF240_07190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7175050.1"
                     /translation="MFFAMTQAPTASAEGPRSRQPHMTVELTNVCNLHCSYCVRDEDD
                     LYHSPANFFPADLLRRIVARAKETWGLRRITFTGGEVTIHPQFGEIVEAVADLGMQFS
                     IVTNGWHFDRVHPLLTKHREALRVVAFSLDGPTREEHDHWRGAGSFTRVVRAMTRCRM
                     TGLRFHLKANVRRDTLPRLQEFALFASRLGAAGLHFAHLLPTSGGVEDESALTQEERR
                     EAEHEIAILSNIFKMPVTILAGYHDIDPAPPCESLDGTACNVDFRGRLTLCCNLSGYR
                     GAAEDSDVVADLTREDFAEGYERLRRVAAEQNERRRRSLASFADSGREADLYTGSPCL
                     FCVQSFGKIPWRGDYTGDIRARSLPVIQNAAR"
     gene            complement(24786..26057)
                     /locus_tag="VF240_07195"
     CDS             complement(24786..26057)
                     /locus_tag="VF240_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7175051.1"
                     /translation="MPTQKLNFDALISPVRAEDFFSDYWERAPLHVRRGLPEAYAPLL
                     DLADLDYLTSVALRMPETLVESLGDPAAGPYVVRPVTAPSELYDACARGASFRLRGLH
                     RYWKPLRDLSRSLEQKFACAVGVNLYSSPASAQGLDRHYDNHDVLVLQIAGRKNWRLH
                     DPIVRLPLKHAPPTSFERRVEELEYFRGGPMRSRGRLTDEETGRPTHELTMEQGDLLY
                     LPRGFVHEARAEGAASVHLTIGLHVTTWLDLLAVALGQLAQRDERFRRSLPVGVLHDA
                     GAASRADFDALVEAFASGASLRDALTEVAEGFVRTRDDAGDGALLGGAGNVDDASKVG
                     LETLLERRPGLVCRVVVEADSVGLASARGALWMPKPFEAALVFVARAEEFRPRDIPGG
                     VSEAGRLALARRLIQDGFVRVAAAREQSREE"
     gene            complement(26161..>27256)
                     /locus_tag="VF240_07200"
     CDS             complement(26161..>27256)
                     /locus_tag="VF240_07200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7175052.1"
                     /translation="VPAGVERVLPDGGDESTAAEQWQSLLTEELLVVGGTPGGLYRLV
                     KLGASKGRAVDWAAEHFDLVVVDEASQMGLAEALTAAAFLREDGQFIAVGDHRQMPPI
                     LAHAWDRESRRDIRHARPHLSIFEYLMSLGFTRAALDESFRIPPEIADFLRRHVYSSD
                     GIDFRSSNTRRLGAAHFEEAWLAAALSPEHALVVIEHDEAGSQQSNEFEARLVERIVG
                     AGCGALGLDAARGGIGIVVPHRAQKALLASRLPHLAESIDTVERFQGGERSLIVVSAT
                     VSDREFAASESSFLLEPRRLTVAVSRPTHKLVVVASRAVFDLIPADLDEYERGALWKH
                     LRHESDARLLWEGDIDGQRVSVRAVGGALL"
BASE COUNT         4307 a         9313 c         9032 g         4604 t
ORIGIN      
        1 cgacaacgct ccgcaaacga acgcgccggc gtcggtggtc gtgccgctct cttcccatag
       61 gcggggcgct tccccctggc ggctcccgcg ctgggcgcag ggcgcggccg tcgcggccgc
      121 ctcggtgctc ctcgtcgtcg gcctctacgg agtcttccgc aagcagtcgc cgcccccggc
      181 gcaggtcgcg cagacggacg tgcaaaagtc gaacgcggtc gtcgagcaga aacaggaagt
      241 gaggtccgca caaatcgcgg acgagggcga cacgacggac gcggcgggag acaggagcgc
      301 cgacgttgtt gagccggagc gacgcgagcc ggaagtgttc acgccgcgca ggaacaatcg
      361 cgcaggcggc gcgcggccca acttccagat ggcgaagttt ggcaacggcg gcgcgcgccg
      421 gccggcagcc acgggcgcgg gcgccgacgc gaccgaggag atcaccacta acttcttccc
      481 tttgatgaac ggcggccagc tcacgcccgg cgacgccggc cacgtcatcc gcgtcgaggt
      541 gccgcgcacg gcgctggcct ccttcggcct gcccgtcaac gccgaccgcg cgggcgggcg
      601 cgtcaaggcc gacgtgctca tgggcgagga cggcatggcc cgcgcgatac gcttcgtgca
      661 gtgacatctt cagagcgagg ggacgtgaat ttttaatagc gggcgtgccc gcggggcaat
      721 gataccgaaa acgattctga gaggagctac agagatgatg ttgatgaaaa aatcgagact
      781 gacgacactc gcgctggccg gcctgatggc caccagcagc ggtgcgggcg tcgtggcgca
      841 gcaacagcag gttaaggaga agcagagaat tcaggaagtc atcgtcggtc aggacggcgt
      901 cgtcatcacg cccgcgccgg gcgggttcgg cacaggcgtc ggcatgggac agggcgccgg
      961 cgtgtcggtc ggcggcggcg accagaactt cgtcttcgtc tcttccgaga tggcgattga
     1021 cggcaaagtg gtcaagggcg cgccatactc ggctgacgcc gtgacggaga cgacgcagac
     1081 gcttgcggac ggcaaccgca tcgtccgcaa gaacaccgcg caggtctacc gcgacagcga
     1141 ggggcgcacg cgccgcgagc acaccatctc ggtcgtcggc ccgttcgcca ccgagggcga
     1201 tccgccgcgc actatcttca tccacgaccc cgtggcctcc accaactata tcctcgaccc
     1261 gcgctcgcgc accgcgcgca aggttaacgc cgttcgcacc ttcgagcgca tgatcgacag
     1321 cggcacgagg ttcaagaccg aagtgttagc gcaggcagag gcgaaggccg ccggcgtcgg
     1381 cgagaccagc ccggcggcga acggtcagga gccgaagcaa agggtgcgct cctccggcta
     1441 cgtctacacc acgccgagcg gcgtcggcca ccatcccggc ggccccgaag tgatgttcta
     1501 caagcacgac ggcgcggagc cgaagatcga gaagctggcg agccagacgg tcgagggcgt
     1561 gttggccgag ggccagagga tcacgcacac catcccggcc ggctccatcg gcaacgagca
     1621 gcccatcaac atcgtcgatg agcgttggta ctcgcccgag ttgcagacgg tcgtgatgac
     1681 ccgccacagc gacccgcgct ccggcgagac cgtcttccgc ctgacgaaca tcaaccgcag
     1741 tgagccggcc cgcacgctct tcgaggtgcc gtccgactac accgtcaggg aagtcccttc
     1801 gggcgtgaac accttccgcc ggaagcgggc cgagggcggc gcgcagaagg agaacaactt
     1861 ccagttccac tacaaccgca acgagcagca gcagcagtag ccgcgtccgc cgaaaggtcg
     1921 gtcatgcacc ggccggggcc gactcggaaa gagaaagggc gaagcgttcg cgtgaacgct
     1981 tcgccctttc tctttgtctc tttgaaacag attcgcggcc cggtcagcag ttcacgtagc
     2041 cgagcaggta gaacacgacg atgaagacga ggatggtcga aatacatccg ccgccgagct
     2101 tccacgccgc gatgccggcc aaaattccgc gcataggtcc ctgcataaat gctctccttc
     2161 cacgaggtcg ttgatggcta ccgggggttt tctgaaaatc tttctaaggc aaaggggccg
     2221 ggatgtcaat gaccttgcgg cgcggtaagt atccacgcgc gctggcgccg gggccgccct
     2281 tgactcataa tgccccctct cgatttcgac cctttaccag acccgatgga taactccccc
     2341 acgaatcatc ccgcgccgcc gccgctgctg cgccgccgct gggccgagat agtctgcgcc
     2401 gccgcgctct tcgtcttcgc cgcgaactcc ttcctcgtca tctcccggaa gaacttcacg
     2461 accgacgagg cgtttatgat tccggccggc tattaccacc tgacggcgcg cgacttccgc
     2521 cccgtcaacg agcacccgcc cctcgtcaag ctgctctcgg ccgtgccgct gcttttcctc
     2581 gaccccgccg cggcgcccca cgagagactg gaggcgcgct tctacagcga ccccaacaac
     2641 ctctactgga ctttctggcg cgacaaccgt gagcgcatcg acgagctttc gttctgggcg
     2701 cgtgtgccgg ccgtcctcct cacggtgctg ctcggcgcgc tcgtcttctg ctacacgcgt
     2761 cgtctcttcg gggcgcgcgc cgccgcgttc gccgtcctcc tcttcacgct ggagccgacc
     2821 gtgctcgctc acggccgcgt cgtgcagacc gacatccccg cctcgctcgg cctcctcctc
     2881 ttcaccttcg cgctctacga ctacctgcgc ggcccttcgg cgcgacgcgc cgcgtgggtc
     2941 ggggcggcgg cggggctggc cgtcgtcaca aagttttcga tggtcgttct cgcgcccgtc
     3001 atcgtcgtcg ccttcgtcgc gctcatctgg cgggcgccgc ggcgcgcgac cgggcggggc
     3061 gcggtcgtcg cgcagttcgc cgtctgcgcg ctcgccgcgc tcctcacgat caacgccgcg
     3121 tatctcttcc aacaccgccc gcccgacgag ggcgacacgg ccatcatcct ctcccgcatc
     3181 gtctccgacc cgtcgagcga gggcctgcgg caatggctcg cgggcgcgta tgcggtggtc
     3241 gtcaaaatca tcccggccga ctttctcgaa ggcgtcggct ggcagctcac gcacgccagc
     3301 gaggggcacg acgcggggct gctcgggcgc tacagcaagc acggctggtg gtactacttc
     3361 cccgtcgcct tcgcgctcaa gacgacgctg cctttcctcc tgctgtccat cgtgggaatc
     3421 gtgtgggcgg tgcgcggctt catgaaagaa ggcggcgggc gctggctcgt tgtgctcgtc
     3481 cccttcgtgc tctacacggc gctcgtcatg tcaagctcga tcaacatcgg cgtgcgttac
     3541 tacctgccgg ccttcccctt cctttgcatc gcggccggcg tctttctcga ccggctcctc
     3601 cggcgacggc ggccggagtc ggagccgcgc ccgcgtcgcg ctctgctcgc gaccgtggtc
     3661 gtcgtcgcgg cgttcgcgtg gatgggcgtg gaggccgcgc gggcgtttcc cgattacatg
     3721 acctacacga accagctcgc cgcggggcgt ccgcactggt ggtatctgtc ggactcgaac
     3781 gtcgagtggg gcgacgacgt ggcggagctt gcccgatatc tgaaggcgcg cggcgagacg
     3841 caggtgcgcg cctccctcct caactacatc gtgctcgaag actacggcgt cggctatcgc
     3901 tgggcctatc tgccgccggg cgcgcccacg cccgcggacg tgcgctacgt cgccatcggc
     3961 gccagctacc tcaacggctc gatcaacccc ggctcactcg aaggccgcct cctctccgac
     4021 gaggagcgcc tcaacctctt cgacgactac cgccgccgca cgcccgagaa aatcttcggc
     4081 cgttcgattt acctctaccg cgtgcgcgac tgaacaaggc acaaggcata agtgtgccag
     4141 ccgggccgga ggcgtcaacg agccgccgaa ggcggcggaa gattgtagcc cacggcgtga
     4201 gccgtgggtc agcggtttag aaaaacaggg aagccccgga ggggcgaaag aaccgcaaca
     4261 ttggcgtggg acgcacgtcg aggctctttc gcccctccgg ggctttaata cttttatcgc
     4321 gtggtcccca cggctcgcgc cgtgggctac attctgccgc ccctggcggg gctgattgac
     4381 gccgtgcgat tcggccctgt cacttttgcc ttttgccttt ttacttttgc cttctcgtcc
     4441 cgagttgact cgcgcccccg cgccgtggaa aatcttccgc gccccggccc atcgtggcag
     4501 cggacaactg attactgacc actgacttca tgaaccactc ttccacactc gttatcctcc
     4561 cgacctacaa cgagcgcgag aacgtcggcg acctcgtcgc gcaaattctc gcttacgccc
     4621 ccgacgcgga cgtgctcctc gtagacgaca actcgcccga cggcaccgcc ccctatgccg
     4681 aggagctatt tgccggcgag ccgcgcttct ccgtgctccg gcgcgagggg ccgcgcggcc
     4741 tgggccgcag ctacgtcgat ggctaccgca cggcgctgga gcgcggctac gcgcggctcg
     4801 tccagatgga cgccgacttc tcgcacgacc cctcgcgcct gcccgacatc ctcactgccg
     4861 cgcgcacggc ggacgtggtc gtcggctcgc gctactgccc cggaggcggc atcggcaact
     4921 ggccactgcg ccgtcgcctg ctcagtcgct tcgccaactc ctacgtctcg gccatcaccg
     4981 gcgtgaaggt cggcgacgcc acgagcggct accgttgcta caaccgcccc gcgctcgaac
     5041 acgtcctcgg caaccgcatc gtcgccgaag gctacgcctt tctcgtcgag tcgatctacc
     5101 gcatcagcga ggcgggcctg aagatcagtg aggtgcccat cgtcttcgtt gaccgacgcg
     5161 agggcaagtc gaagatttcg cgcgcggtga tcttcgagtc ggtgctcatg ccctggaagc
     5221 tgcgcctgcg gccgcgcctg cctgccccgg ccgaccccgc gctggcgcgg gacaactgag
     5281 aagccgtcaa tcgtcaatcg ttaatcgtga ttggttgagc agcttcgctc gaccgcgtga
     5341 cggctccacg tccggggccg ctcaatcacg attgacgatt gacgattcac gattgacggc
     5401 ttttagcatt gactctcggc cgcgtttggc atagaaccct cacgttctac cgaacccttc
     5461 gaccccggag tcttacccca tgaagcgaat acttctcgcg gccgcgttgc tcgccgccgc
     5521 ctccgcgccg gcctgcacgc cgaccaccgg ccccgccaac aactcgaacg ccccggccaa
     5581 ctcgaacgcc gccgccacgc cgacaccggc cccgaccgtc gccgccgccg accacatcgc
     5641 ccgcgagaaa caggtcatgg acgccatcaa gcgcaaggac tgggacgcct tcgccgcgca
     5701 cctcgccgac gaccaactct acgtcagcac cgacggcgtt cacgacaagg ccgccacgct
     5761 cgccagcatc aagaagctcg acatcacgga gtacacgagc gccgacgcgc gtgtcgtcca
     5821 gatcgaccgc gacctcgtcg tcgtcaccca cacgacgacc gcgaagggta cgttcgacgg
     5881 gaagcccatc ccggccggct cgcagcgcga gagcaccgcg tgggccaacc gcggcggtcg
     5941 ctggctcgcc gtctatcatc aggacagcga ggtgatgacg ccgcccacgc cgacgcccgg
     6001 cgcacccacg cccgcggcga ctgccgtcgc cacgccggtc gccgcagcgt cggccactcc
     6061 ggcggccacg cccgccaact cgaccgagct ggagcagcaa atctgggaca cgctcaagcg
     6121 taaggactgg gacgccttcg ccgcgcacct cgccgccgac gcggtcgagg tcggggctac
     6181 cggggtctac gacaaggccg ggacagtcaa cgcggtcaag caggttgact tcaccggcgc
     6241 cacgctgagc gacttcaagg aggtgaagat cgacgcggac gccaccctcc tcatctacac
     6301 ggccaagggg ccacgcgccg tgttcggccc caacggcgaa cgcagcgcca ccatccacgc
     6361 caaccgctcc ggccgctggc tcgccgtctt ccatcaggcg acgtcgatca agtaagcagt
     6421 aagcagtaag cagtaagcag tgagcggtga gcagtcaaaa cattcaagac gtcttgactg
     6481 ctcaccgctc actgcttact gctcacttcc ttcacgccca cacgcccggt atcgtcgtgc
     6541 cgcacgcggg gcacttgccg tcggggccga tcttcatctt gcgaataacg tagccgtagc
     6601 gttcgacgag cggctcgtcg cagttgtggc agtgtgtgtt ctcccagcgg ccgacacggc
     6661 ccgggaggtt gccggcgtag acatagcgca ggccggcggc gcgccccgtc tcgcaggcgc
     6721 ggacgagttg gtcggccgtg gtgttggcgt tctcggtcat ccggtaatcc tgatggaacg
     6781 ccgtcacgtg ccaggggatg tcgggcgaaa cgctcgcgat gaagtcggcg gcgcggcgga
     6841 tttcatcatc cgagtcgttg aagccgggga tgacgagcgt cacgatttcc tcccagaagc
     6901 cgcgctcgcg caccatcctg accgtgtcga ggatgttttc aacgacgccg cccaactgcc
     6961 ggtagttgcg atccgacatc gacttcaaat ctatcttgta gcagtccgtc cacggcctta
     7021 agtagtcgag cacctgcggc gtggcgttcc cgttcgagat gaacgccgtc cgaaacccct
     7081 ccggcttcgc tctcctgaaa acctccaccg cccactccgc cgtaatcaaa ggctcgttgt
     7141 aactcgaccc caccacccgc gccccgtagg cgtgcgccat cgccacgagc cgctcggccg
     7201 tcaccatctt cggcgccgcg cccgccgcgt cgtcgcgcag ggcttggctt gtaatccaat
     7261 tttgacagtt cgacgccgcg aagaagccgg ccaagtagtt gtgctcctct tcgacctcca
     7321 tgttgtaaac gtcgccttcg taatcgacgg aagagacgcc gcggagcggg acgagataaa
     7381 agttgccggc ctcgacggcc ttgcgcccga cgttcgcgcc ttcgtaccac acgaccgtgt
     7441 actggtgagg cgaacgcctc acggcgcggc cctgcacgaa ccctctctcc gactgctccg
     7501 cgtcatacac ggacggcagg tgcccgagtt tcagcgccag ccacgcgacg ccgtaggcca
     7561 gcgagcggga gaccgtggtg atgctgactt tcccgttggc gtagcggtgc ccgtcgcctt
     7621 cgacgtaggc gtcgaggaac gcgcgcgcga tgtggcggtg cgcgttgaag acggccgccg
     7681 gcacccgctt cccggcggcg cggctaccgg ccagagactg aaacaggagc gccgccgagg
     7741 atttgttaca cgtcgcggcc agcgtcgtcg tgcgacgcac gcgcgccgcg cggacgccga
     7801 ggcatttttc gagtagaccc gcgacctctt cgaccagacg gccctcgccg tgagagaagc
     7861 tgaagttgag cgcgtggctg tttgggcgcg tcttgctgct cgtgactgac ccttcggcgc
     7921 agtagagacc gagcagccgg gccatattct catcgagcgg gacggagagc gggatgccgg
     7981 gccggtgctc gttcgggaag cgcaacgtgt cgccttcgat gcgcgggccg ctcgtccgca
     8041 cgtcctgcgc ccggcctcgg gccagcttgc tgcggacgtg acggatgtat gaggcgtctt
     8101 taccgaggcg cgcgccgatg gcgcgtgagg tctcgccgcg ctccgtcgcc acggtaatcg
     8161 agcgccgcac ttcggccgtc aaaccccacg gcacgcggta ggaaattcta tgtccgccca
     8221 actctgccgc cacgttaatc gtctgcggtg ttgagaaaga gtagcggcgc gggattgcga
     8281 ggtagtgccg cgtcgtcagt tgttcggcgc gcgtgagttc gggcgcacgc gtcacgtcgt
     8341 ccgtcgcgta caggcggtgg tcgggcgtgc agcgcagttc cggcaggtaa tagggtcgga
     8401 cggcggcgag tttcccccga tagcggtgtc tgaagacgcc gcgcacgcgc cgaggcgttc
     8461 ctgagacggc cacggcgcgc aggtctttgg gatatgcgat gtcggcgtcc ggccggcgct
     8521 cgacgcgcac ggcagactca aacgcttccg tgaacgtcat cggcccgcgg tcggtgacga
     8581 cgaccgtatc gccggtgaag cagtagccgc agtgaaggtc gcagccgagc atgccgaagg
     8641 tgagggtgtc ggagccgggg aggacgtgga agaagggctt cttctcggtg gggtcgcact
     8701 ggagggcggc gacgtagttg gcgggcacga ggagccggcc ctgttcgttg aagcgcacct
     8761 tgcagatgcc gcggcggccc tcgccgatga gacagcggtg gccgcaggcg tagcagcgaa
     8821 gggcgccgcc cgcgagatgt tcggtcagct cgggcgcgcc ttcgaccgtc atctcgtcca
     8881 ggacttcggc gaggctcgcc gagcgttcgc tcatcggcat acgtgtcgcc ctctcggttg
     8941 ttggcggaag ctcgcggggg ctgtctatac tgacgcgggg gaagactcct tcgcgttatt
     9001 ataactcttc ggggagaggg tttacacggg catgatcatc gagcagataa ccgtcacgtc
     9061 ttttcagcag cacgcgcgca tcgtcgggtg cgacgagacg cgccggtgca tctgcatcga
     9121 ccccggcgac gacaccgagg agatcgtcgc ggcgattgac cgccacggct tcgagctaca
     9181 agccatcgcg ctcacgcacg cgcacctgga tcacgtgggc ggcgtcgccg cgctcaagcg
     9241 ccgccggccc cgtgccgaag tctgtatcca cgccggggac gagccgctct atctgggtct
     9301 gcgcgagcag cccctctggc tcggcatccc gcgctcggag tgggcgcgct acggttttga
     9361 ttatgaggcg ccgcccccgg tcgagcgccg ctgggaggac ggcgaagagt acccggtggg
     9421 ccggctgcgc ttccgcgtca tccactgccc cggccacacg cccggccacg tcgtcctctt
     9481 cgagccggcc cggagggtcg tcttcgtcgg cgactgcctg ttcgccggct ccatcgggag
     9541 gactgacttg ccgggcggct ccaccgagca gttgatggac tcgctgctga acaaaataat
     9601 accgctcggc gacgacgtga cggtctattc cggccacggg ccggagacca ccatcggccg
     9661 cgagcggcag acgaacccgt tcctcacggg cagatatttt tagagtgata agtgatgagt
     9721 gataagtgat aagaaagaac agaagctgtt agctgttggc tgttagctaa gagctaacag
     9781 cttcttatca ctcatcactc atcacttatc actgactttc agcttctcgt tcttcagctt
     9841 gtcccagatg tctttgacct cgcccttgag gtcgcccggc atggtctcga cggggacgtt
     9901 gttctcggcg cagaaggtgc gccagagcag gaagcgcgcg tcgaactccc ccgtcccctg
     9961 gcggatttcg ttcgacagtt gggtcgcgtg cgccgcgtcc agttggctct tttccaggtc
    10021 cggttggctc ttctccacgt ccggttggct cttcacagct tcctgctgga tattctcgtc
    10081 ggccacgttg ttcggttcct cgcttgttcg taatgtcggg ccgcttgggg ttcggacggc
    10141 gcgcgccaca gtttagcagt ttttccgtcc gcgtcggagc gcggccgatt atgaggccgc
    10201 gctgaggaga tacatcttgc cggtcggcgc ggggacgccg ccttcgggtt cgagcgtgac
    10261 ggcgaaggtc tgggcggcga gtccatcggc ggggatgcgg tcgctcagga agccgcggct
    10321 gtccacgttg ggcttgaagg tgccgccggg cacgggcggc tttccgccgt cgatgtacca
    10381 gacctgataa gccttgccgg cgggcgcggg cgggagtccc gaagagatga ggacggcgcg
    10441 gctcgtggcg cggtcatagg cgacggtcgc gtgcgccgcc ggcggcgcgt ccttcgtgcc
    10501 cgcgagcgag gccatgcgcg cttcgggcga ggcgatcaac tcggccaact cgcgcgccca
    10561 ctgaagctcc tcgcgcccgc gttcgagagc ctcctgctgc tgcgcgtcac gactgcgttc
    10621 cgcgttcaac tccgcgccgg agcgcgccag ctcgccgcgc aagtcgttgt tctgtcgcca
    10681 gagcacggcg agcgccaccg ccatcacggc gatgacgagg gaggcggcca gcgcgaacca
    10741 cttcgggatg acaggactgt gcgagggagg gcgggcggcg aactcgagca cgcgcgcgtc
    10801 cccttgcgcc gcgtcggaaa cctctgcgcg cgtctgcgcg gcgggcgacg cgggggcgac
    10861 cgcctcagcg tttgccggcc cgagttcgcg cacgcgctca agcaggcgcg cacgcacgtg
    10921 cccgcccggc gcgacgtgcg gcgtggcgta ggcgagcatc gaggcggcgt cgcgcagagc
    10981 gtcgcgctcg cgcctgcact ccgtgcagga cgcgaggtgc gcctgaagct cgcgcagctc
    11041 ctcgccgtcg agcgcgtcga gcgcgtcgag ggcgagtgcc tctttgtcgt gttcgtcgtt
    11101 gcccatcaaa agttcgcccg cgtgcctcgt caaaaaccgt gtccgagtgt gcgcggtgcg
    11161 cgcgcgctca ctctcgtctc acatcgccgc gcccgcgccc tcgacctgac cccgcaaaag
    11221 ctcgcgcatc ttgagcatgc ctgagcgcat ccgcgtcttc accgttccga gcggctggtc
    11281 gagccgggcg gcgatctccg tctgcgacag cccctcgaag taggcgagca gcagcgcgcg
    11341 ccgctgctcc tcggggatgg aggcgagcgc tccgcgcacg gcctcgccct gctcggagcg
    11401 cacggcttcg tcggaggcgt cggccaccgg ctcgggggcc tcggcggtcg aggccagcgc
    11461 ggcccgctcg cgcgagcgca cagagcgcag gcggtcgatg gcgcggctgc gcgccagagt
    11521 caccagccat gtgaaggggc ggccgcgctc ggcgtcgaag ctcgacgcgc gctgccagat
    11581 ttgtagaaag acctcctgca acacgtcttc cgcctcgggg cggtctttca atatacgcag
    11641 caggagaccg aacaggatgt gactgtaatg gtcataaagc gaggctaacg cccgctcgtc
    11701 gccgcgcgcg acggcgtgga tgagttcggc gtcaactttg tcttgctcgg gtagggcgat
    11761 caaacttttc cgcctcggtg agttttcggc cgccgcgagg ttctcggcct tcgacttcac
    11821 atgtgtccat gccggccacg cgctccgggc cttacgcgtg acttacgacg tgaaatgcct
    11881 cagtggattt cttttagcag aaaaagggtg ctttgtcagc aactccgcac cgcctcgccc
    11941 gcctaaacta aagcgccggc attccgaaga aatgccgacg ctttggtttt tttgttgtgc
    12001 ccggccgccc gtcaggcgac cgtaatctct gcgtcgaggt acacgtcctg gatggcgttg
    12061 aggagttgga cgccctcgtt catcgggcgc tggaacgcct tgcggcccga gatgaggccc
    12121 atgccgccgc cgcgcttgtt gatgacggcg gtgcggacgg cctcggccat gtcgctcgcg
    12181 cccttcgact cgccgcccga gttgatgaga ccgcagcggc ccatgtagca gttggccacc
    12241 tgatagcgga ctaagtcgat ggggttgtcg ctcgtcagct ccgtgtaaat cttcgggttg
    12301 gtcttgccgt aggacgactc cacgttgagc atgttgtagc cgccgttccg ctcgggcagt
    12361 ttctgcttga tgatgtcggc ctgaatggtc acgccgaggt gattggcctg ccccgtcagg
    12421 tcggccgccg tgtgcatgtc gccctccttg cccttgaact tcgggttgcg caggtagcac
    12481 cacaggatgg tcgccatccc gagttcgtgc gcgatggcga acgactcggc gatctcgctc
    12541 aactggcgag ttgattcctc cgagccgaag tagatggtcg cgccgacggc cgccgcgccc
    12601 atgtcgcgcg cctgctcgac ggcgccgaac atgacctgat cgaacttgtt cgggtaggtc
    12661 agaagctcgt tgtggttgac cttgacgatg aagggaatct tgtgggcgta cttgcgcgcg
    12721 accgcgccga gcaccccgaa ggtggaggcg acggcgttgc agccgccctc aatcgccagc
    12781 ttgacgatgt tgtcggggtc gaagtaggcc gggttcgggg cgaacgacgc gccggccgag
    12841 tgctcgatgc cctgatcgac ggggaggatg gacaggtagc cggtgccgcc gaggcggccc
    12901 gcgtcgaaga gcgactggag cgagcgcagc accgaggggt tgcggtcgga ttgcgcccac
    12961 acgcggtcaa cgaagtcggg gccgggcact tgcagcaagt ccttcgagat tttcggcgcg
    13021 ttgaaatcga gcagggcgcg cgcgccatcg ccgagcagtt cttcgatgct gcccggcacg
    13081 gcagggcgtt ttagttgggt agccataagc ttatcgaatc ctccggtgtc gaatctttga
    13141 gcgagcggat tgtcaggggc ggttgcgtgt acgccaacat gatacggaaa agccgcgcgg
    13201 atttccagaa ggcgggaaga cggtgataag tgataagtga taagtgataa gtgataagtg
    13261 ataagtgata agtgataagt gataagtgat aagaaagaaa agaagctctt agctgttggc
    13321 tgttagctaa tcgctaagag ctaactgctt cttatcactc atcacttatc actcatcact
    13381 cctcgggtga gcgcggcgcg cgaggcgagg gcgaggcggg cggcctcggc gggcgtgtcg
    13441 gcgagggcgg tgaggtggcc catctttcgg ccgggtcggg cgtcgcgttt gccgtagagg
    13501 tggagcttta tgtcggggtg agcgagggcg gccgtccagt ccggctcgcc ggccgcccag
    13561 aggtcgccga ggaggttgac catcgcggcg ggccgaagct gcgtggtcga gccgaggggc
    13621 aactggcaga cggcgcgtag ttgctgctcg aactggttgg tcagacaggc gtccacggtg
    13681 aagtggccgg agttgtgcgg gcgcggcgca agctcgttga tgagcagcgt gtggtcgcgc
    13741 gtgaggaaga actcgacgca cattacgccg accacgtcga gcgcatcgag gacgccgcgg
    13801 gccatctcga cggccgcgcg cgacgccgct gcggggacgc agagcgcggg cgcgaccgac
    13861 aggtcgagga tgtgccggcg gtgcgtgttc tcgacggcgc cgtagtgtcg gaactcgccg
    13921 ctccctgagc gcgcggcgac gaccgacagc tcgcactcga aatcgacgaa cgcttcaagt
    13981 atgccctcct gcccgtcgag gccagcgaag gcgtcggccg catcctcggg gcgcgcaaga
    14041 cgcgtctgcc ccttgccgtc gtagccgaag ccggccgtct tcaacacggc cgggcagccg
    14101 acttcgctga gggccgcgcg caggtcttcg agcgaggtga cgtggcggaa ggtggggacg
    14161 gggaagccgc tcgtggagag gaaagttttt tcgcgcaggc gattctgcgt cgtgtggagc
    14221 acacgggctg aggggcggac gggcgcgagt tgcgcggccg cctccacggc cgccgccggc
    14281 acgttctcga actcgaaggt gatacaggcg acgccgcggg cgaactcgcg cacggcgtcg
    14341 aggtcgtcgt aggagtttcg atattcacag tcggcgacct gccccgtggg cgtgttggcg
    14401 tgtggtgaga aggtgtggac gcggtagccc atgcggcgcg cggcgagggc gaacatgcgg
    14461 ccgagctgtc cgctgccgag cacgccgacg gtcgcgccgg ggaggacggg cgaggacgag
    14521 ggcggaggga tgagggatga gggatgaagt gattgactca tcggctcgtt cactcaggca
    14581 cagtaccgcg agcggtagcg agcgggtgcg gctgtgatgc gacgcccgcc ccgcgcacgc
    14641 gagacccgcg aggcttccgc tcgcggtact gtgcttcatc cttgttcttc gagtgtggcc
    14701 gcgagcacct tctccgtctg ctcgcggcgg aactggcgca ggcgctcgcg caagggtggg
    14761 cgggtgttag cgaggatggc gacggcgaga agcgcggcgt tggtcgcgcc ggcctgcccg
    14821 atggcgagtg tgccgacggg cactcccgcc ggcatctgca cgattgacag gagcgaatcg
    14881 acgccgcgca agcaccggct ctcgacgggc acgcccagca ccggcacggt cgtctgcgcc
    14941 gcgaccatcc ccggcaggtg cgccgcgcct cccgcgcccg cgatgattac ttccagagcg
    15001 cgctcctccg cacgggccgc gaactcggcc aacaactgcg gcgtccggtg cgccgacacc
    15061 acgcggcact cgtgcggcac gtcgaattgt cgcaacacgt cggccgcgtg tcgcatcgtc
    15121 tcccagtcgg acgtgctgcc catgatgacg gcgacgagcg gcgagacttc cgcttccgac
    15181 ggattgctgc cctgcgtctc gccgtcagat atggtgttca cttcgcccat gcgcttctca
    15241 gacgaaatgt cacgcgtccc cgctcgcggc agaccgtggc atcacaggta tcgctcgcgc
    15301 agcactttca catggtgcac ctcgtggccg gcgatgatgt gggcgagggc gcgcacgctc
    15361 atctcgttgt cgttggccgt gccgcgacga agccacgccg gctcatcgag gtggctgagc
    15421 aggtcgaggg tggtggcgcg cacgcgggcc agctcgtcgg cgagcgaggc gagcgtgcgc
    15481 gcgtcgaagt ttccgtgctc gacgtagacg ttctgatcca tgccgggcag cgaggccgtg
    15541 tcgccgcggg cgatgcacag cgcgcgatag gcgaagacgc gctcgctgtc aacgaggtga
    15601 ccggcgagtt gcttcatcgt ccacttgccc ggcgcgtagg cgctggcggc gcgctcctcg
    15661 ctgacggccc gcaggatgtc gagcgtgtcg tcgatctgtc gggcgagcgt ctggagcacg
    15721 tcgccctcgg ggacgagtga gacgtagccc tcgaagtccg cggggtattc gtccgcgccg
    15781 ggcggcgcgg cggtgtaggc ggtcattggc gttcgagcct cctcggggca gcgcagcgca
    15841 cgtgcggcgc tcactcggtg ttgagattaa cctcgttgtg tacttcgctt gacggcttct
    15901 gtgcgccggg cacaatcgag ccggtgggcg tctgttcttc gagcttctca atcgggccga
    15961 gttccaccgc cgactcttcc tcgctcgcgt ccggcgcctt cgcctgtcct tcgttttctg
    16021 ccatcgttcg ttctcctgtt gaaagctatt agctgttggc tgttagctgt tagttcacgg
    16081 catacgccga ggggcgcgcc gccgctgagt actcaaaaca ctccgattcg ctgactttga
    16141 gctgatagct aacagctaag agctaacagc ttcctcattc ctgcgcgtgc gagtagcccg
    16201 cgctgccgtc gaaattgtag aggttttcgg tctggatgaa gtcgagaccg gcgtcggccg
    16261 cgcggcggag cagcgccgat atttgcgcgt gcgtcacggt ggcgccgcgc tcggcgtcgg
    16321 agacgaagcg gcagcgccag tggtcggagc agaaggtctc ggccgcgccg ccgggccaga
    16381 ccttgacgcc gcggttggca atcgaggaga cgcgcaaccc ctcgccactc aaattctgca
    16441 tctgctcgcc caactcgtcg ggcgagccgt tcgtccactc gaagtacaca tcgactccga
    16501 ccaaatcctt gttctcgttc ttcaccgtct cgccgacggt ggacgactgt gggccgcccg
    16561 cgaggtcttt cgcgtcggcc gcgcgatagc tcacggcctt gagcgtttcg ggcttctgcc
    16621 ccatgcgctt cgccacggcc tcggcgaact cgcgcgtgcc cactttctcg cggctcacac
    16681 cctccttgta gatgtcgtag gtgtggatgc cctcttcgat ggtgcggagc cacgcgttgt
    16741 gggcgcgttc ggccgcctcc gtctggttga tgtggacgag catcatcacg gcgccgagga
    16801 acaggcccga cgggttggcg aggttctggc cggcgcggcg cggggccgag ccgtggatgg
    16861 cctcgaacat ggcgattgac tcgccgatgt tggcggagcc ggcgaggccg acggagccgg
    16921 cgatctgcgc ggccacgtcc gagagcacgt cgccgtagag attaggcagc acgaccacgt
    16981 cgaagttctc gggcgtgtcg gcgagcttgg ccgcgccgat gtccacgatc cagtgctcgt
    17041 tctcgatgtc gggatactgt gcggcgacct cctcgaagac cttgtggaag aggccgtccg
    17101 tgatcttcat gatgttgtct ttggtgaagc acgtgacctt cttgcggttg ttgcggcgcg
    17161 cgtactcgaa cgcgtagcgc gcgatcttct cggtgccggg ccgcgagatg agcttgaggc
    17221 actgcgtcac ctgccgggtc tgccggtact cgatgccgcc gtacacgtcc tcctcgttct
    17281 cgcgcacaat caccacatcc atcaccgggt gcttcgtctc gataaacggg tgataggaga
    17341 cgcacggccg cacgttcgca taaagtccga aggtcttcct aacggtgacg ttcagactct
    17401 tgtagccgcc cccctgcggc gtcgtaatcg gcgccttgag gaaaacgcgt gtgcgccgga
    17461 gcgagtcgaa cgcgtcgggc gccacacccg ccgggttgcc ggccaagtag accttctcgc
    17521 cgatctcgat ctcctcgatg tctaacgccg cgccggcctc cttcagaatg tggagcgtcg
    17581 cctccataat ctcggggccg atgccgtcgc cgcgggcgac cgtgatggga gtcttctcag
    17641 ccatgatgac cgatgcacct ttcgcattct atggaattaa aagagagacc gccgccgcgc
    17701 ctgactcgcc gcacgtgagc agcaaaaata ttccaattag attgaagtgg agatataaca
    17761 ttgcccggcc gcaaatgcca acgcgccgcc gcccacgagg cggccccgac gggtcagtac
    17821 ccggagcggt agcgacgggg tgcggcgtgc gtgagcgctc aagccggtta tcggcagagg
    17881 cccacccggt cgctaccgct cccggtactg acccgttgtc gcattaagat gtggcgcgtc
    17941 gcctccataa tctcggggcc gatggcgtcg ccgcgcgtcc gtgccggggt cgttcggact
    18001 cacgttgacg ggggcggggg cgcgtgttag ttttcgcgcg ttgtgcggcg gcgtgaattt
    18061 ctggagtcgc cgaacgcgct cgcgccgccg tggcgcgctt gtcttgtagt gaatcccccc
    18121 acagaattcg agttgctgct gtgcctcgcg cgcgtctcgc tgcgtgcgtc ggacgaggcg
    18181 cgcgcgcggg cgattcttgg gcgcggcggc ttcgactggg aactgttcca ctcgctcgcc
    18241 tggcgtcatg ggctgatgcc gctggctttc aggcacctcc tcgaacgctt cgccgcgcac
    18301 gtccctgaag cccatctcgc gcgggcgcgt gaagacttcc gtcacaactc cgcgcgcaac
    18361 ctgctgctcg cctccgagct gcggggcgtg ctcgatgcgc tcgcggccga gggcgtggcg
    18421 gccgtcgcct acaaggggcc ggcgctggcg gtgcaagtct acggagactt gaagctgcgg
    18481 agcttcgttg acctcgacgt gctcgtgcgt cgcgccgacg ccgagcgcgc gggccgcgtg
    18541 ctggcctcgg tcgggtatcg tccgcacctg agtctgacgc cggcgcagga ggcgatgctc
    18601 gcgcgctccg agtgtgaccg tgtgtacttc aaggaggggc ggggcgtgat gctcgaactt
    18661 cattgggccg tcgccccgcc atacttctcc ctcgccctcg aaaccgaaga cgtgctcgac
    18721 ggcgcgacgc gcgtcgagtt gtgcggccgg gaggtcgtca ccccatcggc cgagatgttg
    18781 ctgctgctgc tgtgtgtcaa cggggcgaag gacttgtggc gggcgctgga gccgctgtgc
    18841 gccgtcgccg agctgtcgcg cgcgcgcggc ggcgttgact ggccgcgcgt cgtccgcctc
    18901 gcccgccgcg ccggcgcgct gcggatgctc ttcgtcggcc tcctgctcgc gcgcgacctc
    18961 ctcgacgcgc cgctccccgc cgaggtcgcc cgcctcgccg actccgaccg cgccgcccga
    19021 cgcctcgccg gggaggcgag ccgacgcctt gccgagcggg agacgcgcgc gccggggctg
    19081 gccgaggaga cgcgctttcg ggtcgcggcg cgcgagcgcc tgcgggagcg cgcacgctac
    19141 tgcgcgctcc gtctgctgac gccgacctac agggactgtt cgttcgagtt gccgccggcg
    19201 ctccggttcg tctactacgt cctgaggccg ctacgcctgc tcggcggcgt gctgaagcga
    19261 cccgcgtcga agcctgtgtt atgatgcccg gcgcccgaat ggacttcggg agtccgaaag
    19321 ccgccgccgc agtaaagccc gcgaccaccc gcgaccaacc cgatgatcca gggacgagac
    19381 atcatctaca tctccagcat cgaatggagc ttcctgtggc aggggcatca ggagatcgcg
    19441 ctccggctgg cggcggccgg caaccgcgtg ctctacgtcg agaatacggg cgtgcgctcg
    19501 ccgggcctgc gcgacgcggg ccgcgtcgtc gcgcgcgccc ggcgctgggc gcgcgcggcc
    19561 ccctcgcagg gcgcgcgcga gctggcgccg aacatccacg tctcttcgcc cgtcgtgctg
    19621 ccccccttcg ggggcgccgc gcgccgcgcg ctcaaccgcc gcttcttcct gccgtcgctg
    19681 ctcgccgccg cccgccgcct cgggatgcgc aacccgctcg tctggactta cctgcccaac
    19741 gacacgacgc tcgacctcat ccacatgctc ggcgcggagc gcgccggcct cgtctactac
    19801 tgcgtcgccg acttctcgca gctcaccccg cacccgcggc ggcttgaggc agtcgagcgc
    19861 gagatcgtcg agacggccga cgtggtcttc acgaactgct cggagctgaa gatgaagttc
    19921 gcgccgtgga acccgcacac acacgtctac cccttcggtg ttaacctcga cgccttcccc
    19981 ttcgaggcgc aagcgccctc ggggctgaac ggctccaacg ggaacggcgc cgcgccgccg
    20041 gagcgaggga gcgcgcccac gtcgccagcc ttcgtcgcct cgctgccgcg ccccgtcatc
    20101 ggctacgtcg gcggcatgca ccggcatgtc gatttcggac tcatccggga gatggcgcta
    20161 cgccgccccg agtggtcgtg ggtctgcgtc ggggccttgc aggcggccac gggagatttg
    20221 gccgggctgc cgaacgtcca tctgacgggg cagcgcccgc acgccgagct ggccgactac
    20281 gtccgcggct tcgacgtgtg cgtcgtgccc tacgtcgata gccgctacac ggcgaccgtg
    20341 gtgccgacga agatcaacga gtatctggcc gtcggcaagc cggtggtctc gacggcgctg
    20401 ccccccgtgc gcgagttcaa cgaggagcac cgcggcgtcc tgctcacggc cgaggctgaa
    20461 gccgagagct tcctcgccgc cgtcgagcgg gcgctcgaac taccgcgcga cgaggagacg
    20521 cgccgtcgcc gtcgccgtgt ggccgagcag gctgactggg gcgcgcgctt cacacagatg
    20581 tgcgacctcg tcgaagcgcg gctcgacgcg cggggcgctt ggcagtctcc cgccgcttcc
    20641 ctataatcat ccacctgcgg ttcctttgtc tccccgcaag cgttcctcca cgggcgttac
    20701 accacactga aaggcagaca ctaatcttgg aaagatgtga cacgaaggtg aaggggtcga
    20761 gggcggcgcg cgcgctccgc ctgatgatgg cggcgctcgt gctcggcgtc gccttcggcg
    20821 cggccggcgc gcgcgcacaa caaccctcgc cgacgccgca acagaccacg acgccgccga
    20881 cgcccgcgcc ggcagacgac gcgaagcagc cgactgccgc cgccgaccct cgggagccgg
    20941 ccgccgccgc caaacccggc ttcacactgc gcgtcacgaa gcaggggccg ctgcgcgtct
    21001 acaagctcgt ggcgaagaac gcgaaggtct cagacatcgc ggccgacctc tcgcgccaga
    21061 ttggagcgcc ggtgcagctc agcccgctga tgcagcggca gcgggtcacc gtcgagttca
    21121 gcaacctcga tctggaggcc gcgttgcgct tcctcgcgcc cgtgccgctc gtcgattacg
    21181 tcgcgggcgg ggccgacgcc gcgtcgggac agcccaagcc actggccgtc tacctctacg
    21241 cggtcaacga agcgccgccc gctctgaacg agacggtcaa gagcagctcc gaggcgttcc
    21301 tcatcgaggg ccacacggag gaggggacgg aggagtatga gaagcagaag aaggaggagc
    21361 cgctcgaaat cactttcgcg aacaaccggc tgagcgtgcg cgccgagaag cagccgctga
    21421 gcatcgtgct catcaaggtc gccggcgccc tcggcatccc cttcgagctg aaccacaacg
    21481 tgaccgaggt cgtgaacttg aacttccagc agtaccccgt ggatcaggcg atgcgctcga
    21541 tctcgacctc cgtccacttc ttctaccgcg ccgacctctc gacctccgag aagtatccga
    21601 tccgcctctc cctctcggcc tcggcggcgc gctccggcga caggaactga cgatgcaggg
    21661 acagacgatg agcgacacgc ccgcgccgca cgaccacgtc gtcgccaccg agttcgacgg
    21721 cggcgagggc gtgctcgtcg atctcaactc caagcgttat taccagctca acgagacggc
    21781 gatgctcgtc tggcgctgtc tggagcgtgg gctttcttcc gccgagatcg tcggcgcgat
    21841 gaccgctcgc tacgacgtgt cggccgacca cgccgccggc agcctctcga acgtcctccg
    21901 cagccttgag gcgcacaagc tggtcggcct gcggccctaa acgactcggc cgtgctactc
    21961 cttccggttt cctgcctgcc cggccgcgcc cgtcacctta actgtcgttt acccggacgg
    22021 cgcggccggg cagaggaaat gcattttcag gttgaagtca acgcgacagt tgtatatcct
    22081 ttgcgagcca taattgaagg ggtcgaaaaa atagctgtcg gtcgcgcccg cctcgcacac
    22141 caaaaatgtc cgcgcggaag aaagttcttt tcgaccaacc ttaaccgacc atactccatt
    22201 ccccgaggat ggcgaggaga ataaagactt gagcagacaa gacaacaccc caatgacgga
    22261 actttcttcc gcgcagccga acgacggcga gaccgtatcg gcaaagaagc ctttcgtcga
    22321 gccggtcgtc tccgtaccgc aggatgtcct cgaagccacc gccttcttcc aggggtcggc
    22381 gggcgtcggc ggcggcggcg ccgacggcac tgacggcctg gactaactcc accatttttc
    22441 cgtcccacaa tcttcagaca gacagttccg tgtcgatacc agaagtcggg ccggctccaa
    22501 cccacgggtc ggagccggct tcggaaagat tttatgagat tgcgggccgg cgtctgtcca
    22561 tcgaggccga ggcggggtca gcgggcaatc tcgcctcggc ttatgtccgc ggtttccacc
    22621 tcacccagcg cccacgtcct gacgccgacc agctagacct tcgcttccga gtcgtcgccg
    22681 accagccgcc gctgcccgcc ggcctccggc aattcgagat tcccgagggc gtctgccacg
    22741 ccgaccagcg tcgtcattac ttggacgtgg gcggctcacg cgtcgtcgtc gaggagaggg
    22801 cggcgcgtcg agtcacggtc tggttcggcg aggcggcgcg cgcgcccggc agcttcgccc
    22861 tcatcacgac gatggcctac gccatgcccg cggccctgcg ccgcgtcggc ctctacgacc
    22921 tgcacgcgac cggcatggtc gagcctgaga gcggccacgg cttcctcttc cccggctcgt
    22981 cgggcagcgg caagacgagt ctggccgtca ggctcggggc ggccggctgg cagtacatgt
    23041 cggacgacct gatgctgctc tgcggcgagc ggcccgtcgt cgagatgtgg cccctgcgcc
    23101 ggcactttca ggccgacgcc ctctcgctcg ccaactgccc tctgccacgg cttgaagagg
    23161 ccatcgggat ggtcatcgcg accgacccga acaagcgccg cctcaacccg acgattctgt
    23221 tccccggccg ctttgtggcg acggctaccc cgcgcgtgct ctgcttcccg gtcgtcacgg
    23281 gcgagggcga gagccgcgtg gaggcgttgg ggcaggccga ggcgatgatg cgtctcgtca
    23341 gcatgtgtcc ctggtcgagt tacgacctcg cggcggcccg cgaccacctg cggctcctcg
    23401 gccggctcgt gcggcagtgc aggctgttta cgctacgcgc cggccgcgac attttcgaca
    23461 gccccgagcg cgccggccgt ctgctcgcct cgctcgcctg aatttgttcg tctcgtgttt
    23521 ttcgccatga cgcaagcgcc gaccgcctcc gccgaaggcc cacgctcccg ccagccgcac
    23581 atgacggtcg agctgacgaa cgtctgcaac ctccattgca gctactgcgt ccgcgacgag
    23641 gacgacctct accacagccc cgccaacttc ttccccgccg acctgctgcg ccgcatcgtc
    23701 gcgcgcgcca aagagacctg gggcctgcgc cgcatcacgt tcaccggcgg cgaggtgacg
    23761 atccacccgc agttcggcga gatcgtcgag gccgtggccg atctcggcat gcagttcagc
    23821 atcgtcacca acggctggca cttcgaccgc gtccacccgc tgctcacgaa gcaccgcgag
    23881 gcgttgcgtg tcgtcgcctt cagcctcgac ggcccgaccc gcgaggagca cgaccactgg
    23941 cgcggcgccg gctccttcac gcgcgtcgtg cgcgccatga cgcgctgccg gatgacgggc
    24001 ctccgcttcc acctcaaggc gaacgtccgc cgcgacacgc tgccgcgcct gcaggagttc
    24061 gcactcttcg cgtcccggct cggcgccgcc ggactccact tcgcgcacct gctgccgacc
    24121 tccgggggcg tcgaagacga gtcggcgctg acccaagagg agcggcgcga ggccgagcac
    24181 gagatcgcca tcctctcgaa catcttcaag atgccggtga cgattctggc cggctatcac
    24241 gacatcgacc cggccccgcc ctgcgagtcg ctcgacggca cggcctgcaa cgtggacttc
    24301 cgcggccgcc tcacgctctg ctgcaacctc tccggctacc gcggcgcggc cgaagactcg
    24361 gacgtggtgg ccgacctgac gcgcgaagac tttgccgaag gctacgagag gctgcgccgc
    24421 gtcgccgcgg agcagaacga gagacgccgg cgctccctcg cctccttcgc tgactcgggc
    24481 cgcgaggccg acctctacac cggctcgccc tgtctcttct gcgtccagtc gttcgggaaa
    24541 atcccctggc gcggcgacta cacgggcgac atccgcgcgc gctccctccc cgtcatccag
    24601 aacgccgccc ggtagatttt gccccggcgg ggcgcgaaga ttttcgcccc ggcagggccg
    24661 ggaatgtttc accgtggcgg gggcgcggca cgttttcttc tctcttccca agggccgcgc
    24721 gctggcgcgc ttgcccctgg ctactttcct gacgcccctg ccggggcgat gccgcgagac
    24781 gcgctttatt cttcgcgtga ctgttcgcgg gcggcggcga cgcgaacgaa gccgtcctgt
    24841 atgaggcggc gggcgagggc gaggcggccg gcttcgctga cgccgcccgg tatgtctcgg
    24901 gggcggaact cttcggcgcg cgcgacgaag acgagcgccg cctcgaaggg cttcggcatc
    24961 cacagcgcgc cgcgcgccga cgcgaggccg accgagtcgg cctccacgac gacccggcac
    25021 accagccccg gccggcgctc aaggagtgtt tcaaggccga ccttcgaggc atcatccaca
    25081 ttgcccgcgc cgccgaggag cgcgccgtcg cccgcgtcgt cccgcgtccg cacaaagccc
    25141 tcggccactt ccgtcagcgc gtcgcgcaag gaagcgcccg acgcgaaagc ctcgacgagc
    25201 gcgtcaaagt ctgcgcgcga cgcggcgcct gcgtcgtgga gcacgccgac gggcagcgat
    25261 ctgcggaagc gctcgtcgcg ctgggccaac tgcccgagcg cgaccgccaa caggtcgagc
    25321 cacgtcgtga catgcaaccc gatggtcaaa tgcaccgacg ccgcgccctc cgcgcgcgcc
    25381 tcgtggacga agccgcgcgg caggtagagc aggtcgccct gctccattgt tagctcgtgc
    25441 gtcggccggc cggtctcctc atcggtgaga cggccgcgcg agcgcatcgg cccgccgcgg
    25501 aagtactcca actcctcgac gcgccgctcg aacgacgtgg gcggcgcgtg cttgagcggc
    25561 aggcgcacga tggggtcatg caagcgccag ttcttgcggc ccgctatctg gagcacgagc
    25621 acgtcgtggt tgtcgtagtg gcggtcgagt ccctgcgcgc tcgcgggaga cgagtagaga
    25681 ttgacgccga ccgcgcaggc gaacttttgt tcgaggctcc gcgacaaatc gcgcaacggc
    25741 ttccagtagc gatgcaagcc ccgcaggcgg aacgacgcgc cgcgcgcgca cgcgtcgtag
    25801 agttccgacg gggccgtgac gggacggacg acgtaggggc cggccgcggg gtcgccgagg
    25861 gattccacga gcgtctccgg catacgcagt gcgacgctcg tcaggtagtc gaggtcggcg
    25921 aggtcgagca ggggcgcata agcttccggg agaccacgcc ggacgtgaag cggcgcgcgc
    25981 tcccagtagt ccgagaagaa atcctcggcc ctgacgggcg agatgagcgc gtcgaagttg
    26041 agcttttgag tcggcatgtg actatttgcg cctgacgcgc gaacggcgca ttctacgttc
    26101 ccggcgcggt cgcgtccaaa cgcaatgcgg caagcgcgcg aggcgacggg tgacggctac
    26161 tcaaaggagc gcgccgccga cggcgcggac gctgacgcgt tggccgtcga tgtcgccctc
    26221 ccagagcagg cgcgcgtcgg attcgtggcg gaggtgcttc cagagcgcgc cgcgctcgta
    26281 ctcgtcgagg tctgcgggga tgaggtcgaa gacggcgcgc gaggcgacga cgacgagctt
    26341 gtgcgtgggc cgcgagacgg caacggtgag ccggcgcggc tccagcagga aggagctttc
    26401 cgaggcggcg aactcgcgat cagacacggt cgccgagacg acgatgagcg agcgctcccc
    26461 gccctgaaat cgctcgaccg tgtcgattga ctcggccaga tgcggcaaac gcgacgcgag
    26521 cagcgccttc tgcgcgcggt gcggcacgac gatgccgatg cctccgcgcg ccgcgtcgag
    26581 tcccaacgcc ccgcagcccg cgccgacgat gcgctcgacg agtcgcgcct cgaactcgtt
    26641 cgactgctgc gaacccgcct catcgtgctc gatgacgacg agcgcgtgct cgggagagag
    26701 cgccgctgcc agccacgcct cctcgaagtg ggccgcgccg agacgcctcg tgttcgacga
    26761 gcggaagtcg atgccgtcgg aggagtagac gtgccggcgc aaaaagtcgg cgatctccgg
    26821 ggggatgcga aacgactcgt cgagcgcggc gcgcgtgaag ccgagagaca tcaaatactc
    26881 gaagatggag aggtgcgggc gcgcgtggcg gatgtcgcgc cggctctccc tgtcccatgc
    26941 gtgcgccagt atcggcggca tctgccggtg gtcgccgacg gcgatgaatt gcccgtcctc
    27001 tctcaggaac gcggcggccg tcaaagcctc ggccagcccc atctgcgacg cctcgtccac
    27061 gacgaccaga tcaaaatgct ccgccgccca atccaccgcg cgccccttcg acgcccccag
    27121 cttcaccaga cgatagagtc cgcccggcgt cccgccgacg acgagcaact cctccgtgag
    27181 caacgactgc cactgctcgg cggccgttga ctcgtcgccc ccgtcgggca gcacgcgttc
    27241 gacgcccgcg ggcacc
//
LOCUS       DASVES010000177         4993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_777241, whole genome shotgun sequence.
ACCESSION   DASVES010000177 DASVES010000000
VERSION     DASVES010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777241"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VF240_07205"
     CDS             complement(<1..792)
                     /locus_tag="VF240_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7175053.1"
                     /translation="METTIKEFPYRRVVVTGGAGFLGRYVVERLKALEGVEVVVPRSR
                     EYNLVEGADVSRLLEEARPDMVVHLAAVVGGIGHNQKNPGRFLYENLMMGTQLIEQSR
                     LRGVKKFLALGTVCAYPKFTPVPFREDDLWDGYPEETNAPYGLAKKMMLAQLQAYREQ
                     YGYNGIYLLPANLFGPGDNFDLETSHVIPALIRKCVTARREGAEFIEAWGTGDVSREF
                     LYVEDCADGILRAAALYDDSPPVNIGNGREVFISELVETVARMTGF"
     gene            1069..2118
                     /gene="gmd"
                     /locus_tag="VF240_07210"
     CDS             1069..2118
                     /gene="gmd"
                     /locus_tag="VF240_07210"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7175054.1"
                     /translation="MAKRALITGITGQDGSYLAELLLEKGYEVYGMVRRSSLFNTDRI
                     DHLYQDPHEPGTRLRLIYGDLNDSSSLNQILRQTAPDEIYNLGAQSHVRVSFDVPEYT
                     GEVTGLGAVRILEAIRDLGLRPRFYQASSSELYGKVHETPQSETTPFYPRSPYGCAKA
                     YAYYITVNYRESYGLFACNGILFNHESERRGETFVTRKITRAATRIKLGLQQKLYLGN
                     LDARRDWGHARDYVEAMYLMMQAEEADDYVIATGETHSIREFLDETFGHLQMDWKPYV
                     EIDPRYYRPAEVDLLLGDASKAREKLGWTPKVSFRELARLMVDADMKIAERERAAAER
                     EGTPGGAASARWGAV"
     gene            2206..3234
                     /gene="gmd"
                     /locus_tag="VF240_07215"
     CDS             2206..3234
                     /gene="gmd"
                     /locus_tag="VF240_07215"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7175055.1"
                     /translation="MKRALITGIAGQDGSYLAEMLLEKGYEVFGLVRRSSNVARTRID
                     HLTQGQAAARAGDDPAARIHLIYGDMNDSASLHRAVRESRPQEVYNLASQSHVRVSFE
                     MPEYTVEINAMGTLRLLDAIRDAALETRFYQASSSELFGKAATAPQDEHTPFHPRSPY
                     AVSKLCAYWTVVNYREAYGMYAVNGILFNHESPRRAENFVTRKITLGAARIKLGLQQK
                     LSLGNLDARRDWGFARDYMEAAWMILQQPQPDDYVVATGELHSVREFLDEAFGYLGLD
                     WREHVETDPRQLRPAEVDAVVGDSSKARRVLGWRPRVAFRELVRMMVDADLELAKGEA
                     DKVRATDL"
     gene            complement(3515..4666)
                     /locus_tag="VF240_07220"
     CDS             complement(3515..4666)
                     /locus_tag="VF240_07220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7175056.1"
                     /translation="MQTKNATEEAGVPLLDLKAQYAPLREELLAAVGRVMESQAFIMG
                     EEVRALEEEFALYSQTKYAIGCASGSDALLLALMALGVGAGDEVVTTPYSFFATASSI
                     TRLGASPRFVDIDARTYNIDASQVEDAINERTRALLPVHLYGQCAQMDELTKLGARRA
                     LPLVEDAAQAVGAEERAGRRAGSMSAVGCFSFYPTKNLGGAGDGGMMTTNDDELARRL
                     RILRAHGGETKYHHSEVGINSRLDALQAAVLRVKFPHLDAWSDARAERADHYRRLFTD
                     AGLAEEIALPFVREGVRHIYNQFVIRVVGEGQRDALIEHLKRARVGTEVYYPVPLHLQ
                     ECFRYLGHREGDFPRAERAARETLALPVYPELTPRQQEYVVASIGSFFR"
     gene            complement(4693..>4993)
                     /locus_tag="VF240_07225"
     CDS             complement(4693..>4993)
                     /locus_tag="VF240_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175057.1"
                     /translation="VRVYDSGVTASRAGGGGDAVAAEEEREAAYRTLVSYRTGDVWAP
                     KLDTTEALAHVCREFLDAVRERRAPLTDGHSGLRVVRLLEAAQASIKRGGQPVAV"
BASE COUNT          864 a         1707 c         1600 g          822 t
ORIGIN      
        1 gaaacccgtc atgcgcgcca ccgtctcgac cagctcgcta atgaacacct cgcgcccgtt
       61 gccgatgttg acgggcggag agtcgtcata gagagccgcc gcgcgcagga tgccgtcggc
      121 gcagtcctcg acgtagagga actcgcgcga cacgtcgccc gtgccccatg cttcgataaa
      181 ctcggcgccc tcgcgccgcg ccgtgacgca cttgcggatg agggccggga tgacgtgcga
      241 agtttcgagg tcgaaattat cgccgggccc gaacaaattt gcgggaagca ggtagatgcc
      301 gttgtagccg tactgctctc ggtaggcttg aagctgcgcg agcatcatct tcttggccag
      361 cccgtagggc gcgttcgtct cctcggggta gccgtcccag aggtcgtcct ccctgaaggg
      421 cacgggcgta aatttcggat acgcgcaaac cgtgccaagc gccagaaact ttttgacgcc
      481 gcgcagacgc gattgctcga tgagctgcgt gcccatcatc aggttctcat agaggaagcg
      541 acccggattc ttctggttgt ggccgatgcc gccgacgacg gccgcgaggt ggacgaccat
      601 gtcgggccgc gcctcttcga gcaggcggga aacgtcggcc ccctcgacca gattgtattc
      661 gcggctgcgc gggacgacga cctcgacgcc ctctaacgcc ttcagccgct cgaccacgta
      721 ccggcccaaa aaccctgcgc cgcccgtgac gacgacgcgc cggtagggaa attctttaat
      781 cgttgtctcc atctacagta aacctgaact ctttcgtgtg tgtcggtgtg cggaagtgag
      841 tttacgcgaa gtcggcccgg ctcgaaagtg cgcgctccgc gcccgcgcga gtggcggcgg
      901 aagcgcgcgc gcggcagttg tgcttgacag ccgctccgcc gcttgtccaa actctcctct
      961 ttggctatgg tagcaaactc ttaaccgtac agaaacgcgc cgcgttgcgg gccgcccggc
     1021 tcgcccggcc cgccattcaa acgagagcag taaggacacg accgacgcat ggcaaaacgc
     1081 gcactgataa ccgggatcac gggccaggac ggctcttacc tggccgagtt gctgctggag
     1141 aagggctacg aggtctacgg catggtgcgc cgctcctcgc tcttcaacac cgaccgcatc
     1201 gaccatctct accaggaccc gcacgagccg ggcacccgcc tgcgtctcat ctacggcgac
     1261 ttgaacgact ccagctcgct caaccaaatc ctgcgccaga ccgcgcccga cgagatttac
     1321 aacctcggcg cgcagtcgca cgtccgcgtc agcttcgacg tgcccgagta caccggcgag
     1381 gtcacgggcc tcggcgcggt tcgcatcctc gaagccatcc gcgacctcgg cctccggccg
     1441 aggttctatc aggcgtcctc ctcggaactc tacggcaagg tacacgagac gccgcagagc
     1501 gagaccacgc ccttctaccc ccgctcgcct tacggctgcg cgaaggctta cgcctattac
     1561 atcaccgtca actaccgcga aagctacggc ctcttcgcct gcaacggcat cctcttcaac
     1621 cacgaatcgg agcgccgcgg cgagaccttc gtcacgcgca agatcacgcg cgcggccacg
     1681 cgtatcaagc tcggcctcca gcagaagctc tacctcggca acctcgacgc ccgaagggat
     1741 tggggccacg cccgcgacta cgtcgaggcg atgtatctga tgatgcaggc cgaagaggcg
     1801 gacgactacg tcatcgcgac gggcgagacc cattcgatcc gcgaattcct cgacgagacc
     1861 ttcggccatc ttcagatgga ctggaagccc tacgtcgaaa tcgacccgcg ctactaccgc
     1921 cccgccgaag tcgatctact cctcggcgac gcctcgaagg cgcgcgagaa gctcggctgg
     1981 acgcccaaag tctccttccg cgaactcgcc cgcttgatgg tcgatgcgga catgaagatc
     2041 gctgagcgcg agcgcgccgc cgccgaacgc gagggcacgc ccggcggtgc ggcgtcggcc
     2101 cgctgggggg cggtctgaaa ggagtgataa gtgatgagtg ataagtgata agaaaaaccg
     2161 tccgctcttt cttatcactt atcactcatc acttatcacc gtcatatgaa acgcgccctc
     2221 atcacaggca tcgcggggca ggacggctcc tatctcgccg agatgttgtt ggagaaaggt
     2281 tacgaggtct tcggcctcgt gcgccgctcc agcaacgtcg cgcgcacgcg tatcgaccat
     2341 ctcacgcagg gacaagcggc ggcccgcgcc ggcgacgacc ccgcggcgcg catccacctc
     2401 atctatggcg acatgaacga cagcgcctcg ctgcaccgcg ccgtgcgcga gtcgcgaccc
     2461 caggaggtct acaacctcgc cagccagagc cacgtccgcg tcagcttcga gatgcccgag
     2521 tacaccgtgg agatcaacgc catggggacg ctccgtctgc tcgacgccat ccgcgacgcc
     2581 gctctggaga cgcgcttcta tcaggcgtct tcctccgagc tgtttggcaa ggccgcgacc
     2641 gccccgcagg acgagcacac gcccttccac ccgcgctcgc cttacgccgt ctcgaaactg
     2701 tgcgcctact ggacggtcgt caactaccgc gaggcttacg gcatgtacgc cgtcaacggc
     2761 atcctcttca accacgaaag cccgcgccgc gccgagaact tcgtcacgcg caagatcacg
     2821 ctcggcgccg cgcgcatcaa gctcggcctc cagcagaagc tctcgctcgg caatctcgac
     2881 gcccggcgcg actggggttt tgcgcgcgac tacatggagg cggcctggat gatcctgcaa
     2941 cagccccagc cggacgacta cgtcgtcgcc accggcgagc tgcactcggt cagggagttt
     3001 ctcgacgagg cgttcggcta tctcggcctc gactggcgcg agcacgtcga gaccgacccg
     3061 cgacagcttc gcccggccga ggtggacgcc gtcgtcggcg actcctcgaa ggcgcggcgc
     3121 gtcctcggct ggcggccccg cgtggcgttc agggagttgg tgcggatgat ggtggacgcc
     3181 gacctcgaac tggcgaaggg cgaagccgac aaagtgcgcg cgacggattt ataagccgct
     3241 atagacaccg cccgcccggt gcggcgaaaa ccagccgccg gaggcggcgg cggaatgtag
     3301 cccacggcgt aagccgtggg gacagatgcc agaagatatt gaaagccccg gaggggcgaa
     3361 agaatcgcaa cgagcgcgtg acgccggttg cgattctttc gcccctccgg ggcttcggta
     3421 tttttattcg cacattaacc ccggtctcac gaccggggct acattcttcc gcccctgacg
     3481 gggctgaact accgttcgtc ttcgtcgtct gtcctcaacg gaaaaaactc ccaatcgacg
     3541 cgaccacgta ctcctgttgc cgcggcgtca gctccgggta gaccgggagc gccagcgtct
     3601 cacgggcggc gcgctcggcg cgggggaagt cgccctcgcg gtggccgagg tagcggaagc
     3661 actcctgcaa gtgcagcggc acggggtaat agacctccgt gcccacgcgc gcccgcttca
     3721 gatgctcgat gagcgcgtcg cgctggccct cgccgacgac gcggatgacg aactgattgt
     3781 agatgtggcg gacgccctcg cgcacgaagg gcagcgcgat ctcttcggcg aggccggcgt
     3841 cggtgaagag ccggcggtag tggtcggcgc gctcggcgcg ggcgtcagac cacgcgtcga
     3901 ggtgcgggaa cttgacgcgc aggacggcgg cctggagtgc gtcgagacgg ctgttgatgc
     3961 cgacctcgct gtggtggtac ttcgtctcgc cgccgtgtgc gcgaaggatt ctcaggcggc
     4021 gcgcaagctc gtcgtcgttc gtcgtcatca tcccgccgtc gcccgcgccg cccaggttct
     4081 tcgtcgggta gaaactgaag cagccgaccg cgctcatcga gccggcgcgg cgacccgcgc
     4141 gctcctctgc gccgacggcc tgcgccgcgt cctcgacgag cggtaacgcg cgacgcgcgc
     4201 cgagcttcgt gagttcgtcc atctgcgcgc actgcccgta gaggtgaacg ggcagaagcg
     4261 cgcgcgtgcg ttcgttgatg gcgtcttcga cctgagaggc gtcgatgttg taggtgcgcg
     4321 cgtcgatgtc cacgaagcgc gggctggcgc cgaggcgggt gatggagctg gccgtggcga
     4381 agaagctgta gggcgtggtg acgacctcgt cacccgcgcc gacgccgagc gccatcaggg
     4441 cgagcagcag cgcgtcggag ccggaggcgc agccgatggc atacttcgtc tgcgagtaga
     4501 gggcgaactc ctcttcgagc gcgcgcacct cctcgcccat gatgaacgcc tgcgactcca
     4561 tgacgcgccc gaccgccgcg agcagctcct cgcgcagcgg cgcgtactgc gccttcaaat
     4621 cgagcagcgg gacgcccgcc tcttccgtcg cgttcttcgt ttgcatcaga tgattcggtc
     4681 ttcggggctg cttcagacgg cgacgggctg gccgccccgc ttgatggaag cctgcgcggc
     4741 ttcgagcagg cgcacgacgc gcaggcccga gtggccgtcg gtgagcggcg cgcggcgctc
     4801 gcgcacggcg tcgaggaatt cgcggcagac gtgggcgagc gcctcggtcg tgtcgagctt
     4861 gggggcccac acgtcgccgg tgcggtagct gacgagcgtg cggtaggcgg cctcgcgctc
     4921 ctcctcggcg gccacggcgt cgccgccgcc gcccgcgcgg ctggccgtca ccccgctgtc
     4981 gtagacgcgc act
//
LOCUS       DASVES010000178         9663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_317392, whole genome shotgun sequence.
ACCESSION   DASVES010000178 DASVES010000000
VERSION     DASVES010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317392"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF240_07230"
     CDS             complement(<1..321)
                     /locus_tag="VF240_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX7175058.1"
                     /translation="MSEHIVSRKSYFVVFAALMVLTVVTVLAAQVDFGNEVVNTVIAL
                     SIAVTKALLVVLIFMHVRHSQRIIWVLVAGGFFWLAIMMALTGSDYLSREWIKHVEQQ
                     NPPVD"
     gene            complement(450..1187)
                     /locus_tag="VF240_07235"
     CDS             complement(450..1187)
                     /locus_tag="VF240_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEX7175059.1"
                     /translation="MATHADTLEHGGEAGHHTGHAALQHHFDNMEQQKTATTLGMWLF
                     LIQEVMFFGGLFLCYIVYRTLYPQAFQAASSHLNWKLGAFNTVVLICSSLTMALAVWA
                     AQAGRTRRTQVIFLVLTAVLGFVFLGVKAVEYTDKFNDGLIPMAGWFAPTDPHTLQQW
                     AGINIRHIQMFYWIYFAMTGLHALHMIVGIGIIIPIIWAAWRGRYSPEYYAPVELFGL
                     YWHFVDIIWIFLFPLLYLLGAHFGGGH"
     gene            complement(1279..3015)
                     /locus_tag="VF240_07240"
     CDS             complement(1279..3015)
                     /locus_tag="VF240_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7175060.1"
                     /translation="MSTTSTSNPDQRPFGPASGAEWRAKHPHVNYLNADYGIKSWLLT
                     LDHKRIAILYLVSVTFFFALGGAYAIAIRLELMTPAGDLVQSHTYNKLFTQHGILMVF
                     FFLIPSIPAVLGNFLIPLMIGAKDLAFPRLNLLSWYIYMAGGALTFWALLLGGIDTGW
                     TFYTPYSTSFSNTYVVLAGLGIFVNGFSSILTGLNFIVTIHTMRAPGMTWFRLPLFIW
                     AHYATSLIFVLGTPVIAITILLVALERLAHIGIFDPALGGDPVLFQHLFWFYSHPAVY
                     IMLLPSMGVISELISTHSRKNIFGYSFVAFSSIAIAVFSFLVWGHHMFTSTQSVYAGL
                     IFSVLSYAVAIPSAVKVFNWTATLYKGSISWDAPMLYALFFIGLFVIGGMTGLFLAAL
                     GLDVHVHDTYFVVAHFHYIMVGGALMGFFGGMHNWWPKITGRMYPEGWARFAALVIFV
                     GFNLTFFPQFLLGYMGMPRRYHAYPMDEPLWQVLHVMSTAGASILGVGVMIPMFYFIW
                     SMRYGKFAPANPWPATGLEWRTASPPPTENFKVTPVVTWEAYEFRPIEEMKVVGGDTP
                     DAPVAGENPLTT"
     gene            complement(3076..4245)
                     /gene="coxB"
                     /locus_tag="VF240_07245"
     CDS             complement(3076..4245)
                     /gene="coxB"
                     /locus_tag="VF240_07245"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7175061.1"
                     /translation="MTGLLQQVWVPFAPEQASSFAPEVDALYLYLTLLTLFFSVGISL
                     AIVYFAIKYRRRAPTEVPRPRAGAIALELTWTIIPLMISMTIFVWGASLYFKLYRAPE
                     EAMEIYVVGKQWMWKFQHREGQREINELHVPVGARVRLVMTTEDVIHSFYVPAFRIKM
                     DVVPGRYSYAWFEATKTGKYHLFCAEYCGTNHSGMGGYVYVMEPNEYQAWLSGGATDS
                     PAAQGKKLMQDRGCATCHQEDTVLRAPALRGLFGSDIPLADGSQQRVDEDYVRRSIEE
                     PQAQIHQGFEPIMPTYKGQLSAEQLLQIIAYLRSIGSDTRGGGATMTTTEGRAGGTTT
                     GAGPVTGGIPPDTQRSNPVGPTTPAAGRVGQQPSSTTTGTGQGRRAGGGGANSNR"
     gene            complement(4246..5013)
                     /locus_tag="VF240_07250"
     CDS             complement(4246..5013)
                     /locus_tag="VF240_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7175062.1"
                     /translation="MSIEQKLNEQVPLDAVFRDEAGREVRLGDYFRKGKPVVLALVYY
                     ECPMMCNQILNALSGSLVAVSFTAGTEFEVVTISFDAREGPELAARKKATYMKRYGRG
                     EGGDAGWHFLTGGQESIDRITKAVGFNYVWDEQSKQFAHSSAIMVATPEGKLSHYFYG
                     IDYAPKELRLALVEASAGKIGSPVDQLILFCYHYDPITGKFAPVMNVVRAGGVLTVLG
                     LVALIVVLRRKTRGRGDGDGSSGGERWDAEIDTGGAA"
     gene            complement(5249..5866)
                     /locus_tag="VF240_07255"
     CDS             complement(5249..5866)
                     /locus_tag="VF240_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175063.1"
                     /translation="MASGKRRKYDDIHMTPDVSYITNPDVAHEESDVSVGPIAKFVLG
                     LFIFSVVICALMYVMFLFFERRAVQGERRPSPLARRGEAALPPNPRLQASKGFVLDDS
                     QDPSDVLTPQEQDFQLREPQAEYRALLKIWEQDLHGYGWMNQAEGTARVPIERAIQLY
                     AEQQRQKRQQQGQPPQQPGAPTPSAPVEQTPAASSSGRTGEAKNQ"
     gene            complement(6007..7266)
                     /locus_tag="VF240_07260"
     CDS             complement(6007..7266)
                     /locus_tag="VF240_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175064.1"
                     /translation="MDAKANYAAPRIVADWRSRALIIGGVALLLSALVSLLLPPEGTA
                     GLVQFFRSYLVGFFFCTGLAVGSLAWLLLGHMTGGAWALVIRRILEAATRTLPLLLVL
                     FIPVVIALFVHPEGQSLYEWTHHESVERDPALRHKSAYLNAPFFIVRGLIYFGIWILF
                     ARLMNRWSLEQDGTGDPRVRRKMQDLAGPGLLLFGLTATFAAVDWGMSLEPHWFSTMY
                     GLIAMAGWGLSALAFAITVAVALREHEPMAHAYLPHHSHDHGKLLLAMVMLYAYFSFS
                     QFLIIWAGNLPEETQFYLKRLRGGWQYVALALILLHFALPFALLLSRGLKRRGSVLVR
                     VALLMLLMRVVDLVFLFAPATQHGAVVQGHQAAGIHWQDFVTMLLAVVGVGGIWVWHF
                     LGELRKRPLLPVGAPDLAAALEAPAHH"
     gene            complement(7268..8173)
                     /locus_tag="VF240_07265"
     CDS             complement(7268..8173)
                     /locus_tag="VF240_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7175065.1"
                     /translation="MWNTKKRGSQETEVRSQKKDGARRRVLFCLLTSVSCLLSFGCRM
                     DMQDQPRYEAYERGDAKTFPDGQSSRQLVEGTVPRMPAGQPYVDKQSEYFYTGRMSGA
                     QQQQQGGGAPPTGVGAQFGMAGAGMQSTGQGTTTTTTTAAGGGATPGGGAPAATQASA
                     RLGGADIFPTQVTIDEAALKRGQDRYNNFCSMCHGLTGDGDGMIVRRGFQRPPSLHDA
                     GLQEPQASAAHVFQTITNGLGAMPSYATMIPPEDRWKIVAYLRAMQLSRRVNAGELSA
                     DERGKIGAPKQQPGGTQPARGGEQH"
     gene            complement(8160..8711)
                     /locus_tag="VF240_07270"
     CDS             complement(8160..8711)
                     /locus_tag="VF240_07270"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX7175066.1"
                     /translation="MALKKRVRPDTYGLMAEFDNPTDAVAAARRVYDEGYRRIDAFSP
                     YPVEELSEAIGVHSSKMPLIVLIGGIVGMLGGFALQYWTHVIEYPLNVGGKPFFSWPA
                     FVPITFETTVLGAALAAVFGMLALNGLPEPYHPVFNTPNFALASRDRFFLLVESTDPK
                     FDRDETRGFLKGLGALEVSDVEH"
     gene            complement(8839..>9663)
                     /gene="nrfD"
                     /locus_tag="VF240_07275"
     CDS             complement(8839..>9663)
                     /gene="nrfD"
                     /locus_tag="VF240_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7175067.1"
                     /translation="IPDLATLRDRARSKFGRVVYGMAALGWRGSARHWARYEMTYLLL
                     AGLSTPLVLSVHTIVSFDFAIGIIPGWHATIFPPYFVAGAIYAGFAMVLTLVIPLRKF
                     YGLEDFITDRHLRNAAKVMLATGLIVAYGYMMETFMAWYSANTYEEFMIRNRFVGPYA
                     FYYWLLIFCNVVVPQLLWIRSLRHSNVMLFVVAMVVNVGMWLERFVIIPISLHRDFLP
                     SSWGMYQPTIFDFSMFFGTIGFFFFLMFLFLRFLPVISIFEMRTLVPEAKIEEEKG"
BASE COUNT         1883 a         3031 c         3141 g         1608 t
ORIGIN      
        1 atcgacgggc gggttctgct gctcgacgtg cttgatccat tcgcggctga ggtagtcgct
       61 gccggtgagc gccatcatga tggcgagcca gaagaagccg ccggcgacga gcacccagat
      121 gatgcgctgg ctgtggcgga cgtgcatgaa gatgagcacc acgagcagcg ccttcgtcac
      181 ggcgatggag agcgcgatga cggtgttgac gacctcgttg ccgaagtcca cctgcgccgc
      241 cagcaccgtc accaccgtca gcaccatcag cgcggcgaag accacgaaat aggacttgcg
      301 agagacgatg tgctcagaca tattttttag tgataagtga tgagtgataa gtgataagaa
      361 tctgttagct cttagctgtt ggctgttagc taatcgctaa gagctaacag ctcccgttct
      421 ttctcatcac ttatcactta tcacctcctt cagtgccccc cgccgaagtg cgcgccgagc
      481 aggtagagca gggggaagag gaaaatccag atgatgtcaa cgaagtgcca gtagaggccg
      541 aatagctcga cgggggcgta gtactcgggc gagtagcggc cgcgccacgc cgcccagatg
      601 atggggatga tgatgccgat gccgacgatc atgtgcaggg cgtggaggcc cgtcatggcg
      661 aagtaaatcc agtagaacat ctggatgtgg cggatgttga tgccggccca ctgctggagc
      721 gtgtgggggt cggtgggcgc aaaccagccg gccatgggga tcagcccgtc gttgaactta
      781 tcagtgtatt cgacggcctt gacgccgagg aacacgaagc ccagcacggc cgtcagcacg
      841 aggaagatca cctgcgtccg gcgcgtgcgc cccgcctgcg cggcccacac ggcgagcgcc
      901 atcgtcagcg agctacagat aaggacgacc gtattgaagg cgccgagctt ccagttgagg
      961 tgggagctgg cggcctggaa cgcctgcggg tagagcgtgc gatagacgat gtagcagagg
     1021 aagaggccgc cgaagaacat cacctcctgg atcaggaaga gccacatccc cagcgtggtg
     1081 gccgtcttct gctgctccat gttgtcgaag tggtgctgga gcgccgcgtg cccggtgtgg
     1141 tgaccggctt cgccgccgtg ctccaacgtg tccgcgtgtg tggccaaaac aacctccgaa
     1201 accagtgata agtgatgagt gataagtgat aagaaagaga aagacgggtt tctcttatca
     1261 cttatcactc atcacttatc acgtcgttaa aggattctcc ccggcgacgg gcgcgtcggg
     1321 cgtgtcgccg ccgaccacct tcatctcctc gatgggccgg aactcgtatg cctcccacgt
     1381 caccacgggc gtcaccttga agttctccgt gggcggcggg gaagccgtgc gccactcaag
     1441 gcccgtcgcg ggccacgggt ttgcgggtgc gaacttgccg tagcgcatcg accagatgaa
     1501 gtagaacatc gggatcatca cgccgacgcc gaggatggaa gcgccggccg tgctcatcac
     1561 gtgcaagacc tgccagagcg gctcgtccat cgggtaggcg tgatagcggc gcggcatccc
     1621 catgtagccc aaaagaaact gcgggaagaa cgtcaggttg aagccgacga agatgacgag
     1681 cgccgcaaaa cgcgcccagc cttcggggta catccgcccc gtgatcttcg gccaccagtt
     1741 gtgcatcccg ccgaagaagc ccatcaaggc gccgccgacc atgatgtaat ggaagtgcgc
     1801 gacgacgaag taagtgtcgt ggacgtgaac gtcgaggccg agcgcggcga ggaacagtcc
     1861 cgtcatgccg ccgatgacga agaggccaat gaagaagagc gcgtagagca tcggcgcgtc
     1921 ccacgagatc gaccccttgt agagcgtcgc cgtccagttg aagaccttga cggccgaggg
     1981 aatcgccacc gcataggaga gcacggagaa gatgaggccg gcgtagaccg actgcgtgct
     2041 cgtgaacatg tggtggcccc acacgaggaa gctgaacacg gcgatggcaa tcgacgagaa
     2101 cgcgacgaac gagtagccga agatgttctt gcgcgagtgg gtggaaatta gctccgagat
     2161 gacgcccatc gagggcagca gcatgatgta gacggccggg tgcgagtaga accagaagag
     2221 gtgctggaac aggacggggt cgccgccgag cgccgggtcg aagatgccga tgtgcgcgag
     2281 tcgttcgagc gcgacgagaa gaatcgtaat ggcgatgacg ggcgtgccga ggacgaagat
     2341 gagactcgtg gcgtagtgcg cccagatgaa aagcggcagg cggaaccacg tcatgccggg
     2401 cgcgcgcatc gtgtggatgg tgacgatgaa attcagcccc gtcagaatcg acgagaagcc
     2461 gttgacgaag atgcccaagc ccgcgaggac gacgtaagtg ttcgagaacg aggtcgagta
     2521 gggcgtgtag aacgtccagc cggtgtcgat gccgcccagc agcaaggccc agaaggtcag
     2581 cgcgccgccg gccatgtaga tgtaccagct caggaggttg agacgcggga aggccaaatc
     2641 cttcgcgccg atcatgagcg ggataaggaa gtttcccaac accgccggaa tcgacggaat
     2701 caggaagaag aagaccatca ggatgccgtg ctgcgtgaac agcttgttgt aggtgtgcga
     2761 ctggacgagg tcgccggccg gcgtcatcaa ttccagacgg atggcgatgg cgtatgcgcc
     2821 gccgagggcg aagaagaagg tgacgctgac gagatagagg atggcgatcc tcttgtggtc
     2881 gagcgtcagc agccacgact tgatgccgta gtcggcgttc aggtagttga cgtgcgggtg
     2941 cttcgcgcgc cactcggcgc cggacgccgg cccgaacggt ctctgatccg ggttgctggt
     3001 gctcgtcgtg ctcataatcc tctcgacctg ccctactgac cccttgccct tgaaaaactt
     3061 ttcgcgccgc cttcgttagc gattggagtt cgcgccgccg ccgcccgcgc gccggccctg
     3121 ccccgtgccc gttgtcgtgg aagaaggctg ttggcccacg cgcccggcgg cgggcgtcgt
     3181 cggccccacg gggttcgagc gctgcgtgtc gggcgggatg ccgcccgtca ccggccccgc
     3241 gcctgtagtc gtgcccccgg cgcggccttc agtcgtcgtc atcgtcgctc cgccgccccg
     3301 tgtgtcgctg ccgatggagc gcaggtaggc gatgatctgg agtagctgtt cggccgagag
     3361 ctgccccttg taggtcggca tgatcggctc gaagccctga tgaatctgcg cctgcggctc
     3421 ctcgatggag cggcggacgt agtcttcatc gacgcgctgc tgcgagccgt cggcgagcgg
     3481 gatatcgctg ccgaagagac cgcgcagggc cggcgcgcgc aacaccgtgt cctcctgatg
     3541 acacgtcgcg cagccgcgat cctgcatgag cttcttacct tgcgcggcgg gcgaatcggt
     3601 cgccccgccc gagagccacg cctgatattc gttcggctcc atgacgtaga cgtagccgcc
     3661 catccccgag tgattcgtgc cgcaatactc ggcgcagaac aggtgatact tgcccgtctt
     3721 ggtcgcctcg aaccacgcgt aggagtagcg gcccggcacc acgtccatct tgatgcggaa
     3781 ggccgggacg tagaagctgt ggatgacatc ctcggtcgtc atcacgaggc ggacgcgcgc
     3841 gccgacgggg acgtgcaatt cgttgatctc gcgctggccc tcgcggtgct gaaacttcca
     3901 catccactgc ttgccgacga cgtagatttc catcgcctcc tcgggcgcgc ggtagagctt
     3961 gaagtagaga ctcgcgcccc agacgaagat cgtcatcgag atcatcaacg ggataatcgt
     4021 ccacgtcagc tcaagcgcga tggccccggc gcgcgggcgc ggcacctcgg tgggcgcgcg
     4081 tcgtcggtac ttgatggcga agtagacgat ggccagagag atgcccaccg agaagaagag
     4141 tgtgagcagc gtcaggtaga ggtagagggc gtccacctcc ggcgcgaagc tggaagcctg
     4201 ctcgggcgcg aacggcaccc acacctgttg caacagtccc gtcacctaag ccgctcctcc
     4261 ggtgtcaatc tccgcgtccc aacgctcgcc gccgctgctc ccgtcgccat ctccgcggcc
     4321 gcgcgtcttc cgccgcagca cgacgatgag cgcgacgagt cccaaaacgg tcaacacccc
     4381 gccggcccgc accacgttca tcacgggcgc aaacttcccc gtgatggggt cgtagtggta
     4441 gcagaagaga atcaactgat cgacgggcga gccgatcttc cccgccgacg cttcgaccaa
     4501 agcgagccgc agctccttcg gcgcgtagtc gatgccgtag aagtagtgcg aaagttttcc
     4561 ttcaggcgtc gccaccatga tggccgacga gtgcgcgaac tgtttcgact gctcgtccca
     4621 cacgtagttg aagcccaccg ccttcgtgat ccgatcaata gactcctgcc cacccgtcag
     4681 gaagtgccac ccggcgtcgc ccccctcgcc gcggccatag cgtttcatgt aggtggcctt
     4741 cttgcgcgcc gccagctcag gcccttcgcg cgcgtcgaag ctaatggtga cgacctcgaa
     4801 ctccgtcccg gccgtaaacg acacggccac gagcgagccg gagagcgcgt tcagaatctg
     4861 gttgcacatc atcgggcact cgtagtagac gagcgcgaga acgacgggct tgcccttcct
     4921 gaaataatcg ccgagccgga cttccctccc cgcctcatcg cgaaagacgg cgtctaacgg
     4981 cacctgctca ttcaacttct gctcgatgct caccgacttc aaaacttccg gcgtcccgcc
     5041 ccgcggcagg ctgctctcgg gcgggcgacc gtactgtgcg tgcgccggga cggtgcagca
     5101 cagaagtaaa aaggcaaaag taaaaaggca aaaggagtgc gccgcgcggt gcgttttgag
     5161 tccgcgacct gacgcatgcg ttcctgtttt ctcgtaactg ctcattactg ctttcacttt
     5221 tgccttttgc ctttttactt tttccttctc actgattctt cgcttctccc gtgcgccccg
     5281 agcttgaggc cgcgggcgtc tgctcgacgg gagcggaagg cgtgggcgcg ccgggctgtt
     5341 gcggcggctg gccctgctgc tgtcgcttct gccgctgttg ctcggcgtag agctgtatgg
     5401 cgcgctcgat ggggacgcgg gcggtgccct cggcctgatt catccagccg tagccgtgga
     5461 ggtcttgctc ccaaatcttc agcagggcgc gatactcggc ctgcggctcc cgcagttgaa
     5521 agtcctgctc ctgcggcgtg agcacgtccg aagggtcctg gctgtcatcg agcacgaagc
     5581 ccttcgacgc ctgcaggcgc gggttgggcg ggagcgccgc ctcgcccctg cgggccagcg
     5641 ggctgggccg ccgctcgcct tgcaccgccc gccgctcgaa gaagaggaac atcacgtaca
     5701 tcagcgcgca gatgacgacg gagaagatga acaaccccag gacgaacttc gcgatggggc
     5761 cgaccgacac gtccgactcc tcgtgcgcca cgtccgggtt cgtgatgtac gacacgtccg
     5821 gcgtcatgtg gatgtcgtcg tacttcctgc gcttgccgct cgccataacc cctccgaagt
     5881 gagcagtgag cagtaagcag tgagcagtta aaaactgccg cttccgccac gctcaccccg
     5941 tcacttaatc tccacacgtc ataaaaaaac tactgctcac tgctcactgc tcactgctca
     6001 ctttcctcag tggtgcgccg gcgcttctaa ggccgccgcg aggtcgggcg cgccgacggg
     6061 gagcagcggg cgctttcgca gctcgccgag gaagtgccag acccagatgc cgccgacgcc
     6121 gacgacggca agcagcatcg tcacgaagtc ctgccagtga atccccgcgg cctgatgccc
     6181 ctgcacgacc gcgccgtgct gcgtggcggg cgcgaagagg aagaccagat cgacgacgcg
     6241 catcaagagc atcagcagcg ccacgcgcac gagcacgctc ccgcgccgct tcagcccgcg
     6301 cgacagaagc aacgcgaagg gcagcgcgaa gtgcaggaga atgagtgcga gtgcgacgta
     6361 ctgccagccg ccgcgcagcc gcttgagata aaactgcgtc tcctcgggca ggttccccgc
     6421 ccagataatc aggaactgcg agaaggagaa gtaggcgtag agcatgacca tcgccagcaa
     6481 aagcttgccg tggtcgtgcg agtgatgcgg caggtaggcg tgcgccatcg gctcgtgctc
     6541 gcggagcgcc acggcgaccg tgatggcgaa ggcgagcgcg gagagtcccc agccggccat
     6601 cgcgatgagg ccgtacatgg tcgagaacca gtgcggctca agcgacatcc cccaatcgac
     6661 ggcggcgaag gtcgccgtga gtccgaagag gaggaggccc ggccccgcga ggtcttgcat
     6721 cttgcgccgg acgcgcgggt cgcccgtccc gtcctgctcc agcgaccaac ggttcatcag
     6781 ccgcgcgaag agaatccaga taccgaagta gatcaggccg cggacgatga agaacggggc
     6841 gttcaggtac gcgctcttgt gccgcaacgc cgggtcgcgc tcgaccgact cgtgatgcgt
     6901 ccactcgtag agcgactgtc cctcggggtg gacgaagagg gcaatgacga ccgggataaa
     6961 gagcacgagc agcagcggca gcgtccgcgt cgcggcctca aggatgcggc ggatgacgag
     7021 cgcccacgcg ccgcccgtca tgtgcccgag caaaagccac gcgaggctgc ccaccgcaag
     7081 gcccgtgcag aagaagaagc cgacgaggta ggagcggaaa aactggacga ggccggccgt
     7141 gccctcgggc ggcaggagca gcgagacgag ggccgagagc aggagcgcga cgccgccaat
     7201 gatgagcgcg cgggagcgcc agtcggcgac gattcgcgga gccgcgtagt ttgccttcgc
     7261 gtccatccta gtgctgctca cctccgcggg cgggctgtgt gccgcccggc tgctgcttcg
     7321 gcgcgccgat tttcccgcgc tcgtccgccg aaagctcacc ggcgttgacg cgccgcgaca
     7381 gttgcatggc gcgcaggtag gcgacgatct tccagcggtc ttcgggcgga atcatcgtgg
     7441 cgtaggaagg catcgcgccg aggccgttgg tgatggtctg gaagacgtgc gcggccgacg
     7501 cctgcggctc ctgtaggccg gcgtcgtgca aagagggcgg gcgctgaaag ccgcgtcgca
     7561 cgatcatgcc gtcgccgtca cccgtcaggc cgtgacacat cgaacagaaa ttgttgtagc
     7621 gatcctgccc gcgtttgagc gccgcctcgt caatcgtcac ctgcgtcggg aagatgtccg
     7681 cgccgcccag acgcgccgac gcctgcgtgg ccgctggagc accgccgccc ggcgtcgcgc
     7741 ctccgccagc agcggttgtt gttgtcgtcg tggttccctg ccccgtgctt tgcatcccag
     7801 cgccggccat cccgaactgc gcccccactc ccgtcggcgg cgcgccgccg ccctgttgct
     7861 gttgttgcgc cccgctcatg cgccccgtgt aaaaatactc gctctgcttg tcaacataag
     7921 gctgccccgc cggcatccgc ggcaccgtcc cctcgacgag ttgccgcgac gactgaccat
     7981 ccgggaaagt cttcgcgtcg ccccgctcgt aagcctcgta gcgcggctga tcctgcatat
     8041 ccatccggca accaaaagac aggagacagg agacagaagt cagaagacag aagaggacgc
     8101 gacgccgcgc gccgtccttc ttctgactcc tgacttctgt ctcctgactt cctctcttct
     8161 tagtgttcca catcgctcac ctcaagcgcg ccgaggccct tcaggaagcc gcgcgtctcg
     8221 tcgcggtcga acttcgggtc ggtcgattcc acgagcagga agaagcggtc gcgcgacgcc
     8281 agcgcaaagt tcggggtgtt gaagaccggg tggtacggct ccggcaaacc gtttaaggcg
     8341 agcatgccga agacggccgc gagcgccgcg cccagcacgg tcgtctcgaa cgtgatcggg
     8401 acgaacgccg gccaactgaa gaagggcttg ccgccgacgt tcaaggggta ctcgatgacg
     8461 tgcgtccagt actggagcgc gaagccgccg agcatgccga cgatgccccc gatgagcacg
     8521 atcagcggca tcttcgacga atgcacgccg atggcctccg acagctcctc gacggggtag
     8581 ggcgagaagg cgtcgatgcg ccggtagccc tcgtcgtaga cgcgccgggc cgccgccacc
     8641 gcgtccgtag gattgtcgaa ctcggccatc agcccgtagg tgtcgggacg cactcgcttc
     8701 tttaacgcca cgagccacct cctcgaaagg gatgagggat gagggcggag ggatgaacaa
     8761 aaagcacgcc cccgcccaca cgcccgccct tcatccctcc gcccttcatc cctccgccct
     8821 catccctcat ccctttcctc atcccttctc ctcctcgatc ttggcctcgg gcacgagcgt
     8881 gcgcatctcg aagatggaga tgacggggag gaagcggagg aagaggaaca tcaggaagaa
     8941 gaagaacccg atggtgccga agaacatcga gaagtcgaag atcgtcggct ggtacatgcc
     9001 ccacgacgac ggcaggaagt cgcggtggag gctgatcggg atgatcacga aacgctccag
     9061 ccacatgccg acgttgacga ccatcgccac gacgaagagc atcacgttgc tgtggcgcag
     9121 actcctgatc cacagcagtt gcgggacgac gacgttgcag aagatgagca gccagtagta
     9181 gaacgcgtag gggccgacga agcggttgcg gatcatgaac tcttcgtagg tgttggcgct
     9241 gtaccacgcc atgaaggtct ccatcatgta gccgtaggcg acgatgagac ccgtggccag
     9301 catgaccttc gcggcgtttc gcaggtggcg gtcggtgatg aagtcttcga ggccgtagaa
     9361 cttccgcagg ggaatgacca gcgtcagcac catcgcgaag ccggcgtaga tggccccggc
     9421 cacgaagtag ggcgggaaga tggtcgcgtg ccagccggga atgatgccga tggcgaagtc
     9481 gaagctgacg atggtgtgga cggagagcac gagcggcgtc gagagaccag cgaggagcag
     9541 gtaggtcatc tcgtaacgcg cccagtggcg cgcgctcccg cgccagccca gagcggccat
     9601 gccgtagacg acgcgcccga acttcgagcg cgcgcggtct ctcaaggtcg cgagatcggg
     9661 gat
//
LOCUS       DASVES010000179         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_5216, whole genome shotgun sequence.
ACCESSION   DASVES010000179 DASVES010000000
VERSION     DASVES010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5216"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VF240_07280"
     CDS             <1..264
                     /locus_tag="VF240_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7175068.1"
                     /translation="SELSGGEQQRVAIARALANRPRVLLADEPTGNLDSTRAHELLAL
                     LRGMVASDRLTVLMVTHDRELAARFADRTISLKDGKVETMSSQ"
     gene            414..1268
                     /locus_tag="VF240_07285"
     CDS             414..1268
                     /locus_tag="VF240_07285"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX7175069.1"
                     /translation="MTLEALRSVPLFATLDDERAGDLRELLDVRHASAGERLFRAGSM
                     GDAMYFIEDGRVRIHLRDEDGDDVTLAELARGDFFGEMSILDGKPRSADATAASAARL
                     AVLTRDDFLHFVRANPDVALGMLSAIAERLRQTDELLRRRVTRNVNEEYEERRTAADR
                     MADAVSSFGGSWTFVGATALFVAGWVFVNSWVQAERGIDPFPYSFLDVINGIIAALVA
                     PFILISQNRQQEKDRLRSDLDFQVNLKNEMALNELLRRLDVLESERLPLLFREQNERL
                     EKTVSSKQ"
     gene            1597..2298
                     /locus_tag="VF240_07290"
     CDS             1597..2298
                     /locus_tag="VF240_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7175070.1"
                     /translation="MIQAVLFDFNGVVIDDEPLQMKAFQEVLRGEGMSLTEEEYYGSL
                     GMDDATFVRAAFERAGREADAAKLEEFIDRKTEIHRALMKDELPIFPGVVNFIKALRH
                     RYSLGLVSMARRSEIDYALERAGLQNAFEVMVSAEDVQVCKPDPACYNRALTLLNETR
                     RSRAHVLPLRPAECLAIEDSPPGVSAARAAGMRTLAVTNTVAERLLREAGAEIVTHSL
                     ADWTTDAVHHVYDKR"
     gene            2434..>3278
                     /locus_tag="VF240_07295"
     CDS             2434..>3278
                     /locus_tag="VF240_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX7175071.1"
                     /translation="MTPADKVVAASARSRFALEVLRRGEEINLARAALLVAAEEDGSC
                     DVEASLETLARMGREARERVEGERGGAPIVALNRYVFEELGFAGNREKYYDPRNSLLN
                     RVVERRTGIPITLSIVYMELGRLAGLEVEGVGMPGHFIVRARGVEGAPVLVDPFNREI
                     VDEEDCQHRLDEVYGGQAPLTAEHLRSVSKREILIRLLTNLKSVYAQGQLYRRALATV
                     ERILLLAPHALSERRDRGLLLAQLERLPEAVADVQAYLRLNENAPDVESLREQLKKIQ
                     ARQAML"
BASE COUNT          583 a         1121 c         1012 g          562 t
ORIGIN      
        1 tcggagctat cggggggcga gcagcagcgg gtcgctatcg cccgcgccct cgccaaccgc
       61 ccgcgcgttc tcctcgccga cgagccgacg ggcaatctcg actcgacgcg cgcgcacgaa
      121 ctcctcgctc tcctgcgcgg catggtcgcc agcgaccgcc tgaccgtcct catggtcacg
      181 cacgaccgcg agctggccgc gcgcttcgcc gaccgcacca tctctctcaa ggacgggaaa
      241 gtggaaacga tgagtagtca gtaatcagta gtcagtagtc agtagttttc gtttcacggc
      301 ttgaaccttt cattgtcagg gtcaattggt gctacacaaa caactaacaa ctgacaactg
      361 actactttcc ccacgaggtg caacccgccg tgtccgatga tgcaacttca agaatgacgc
      421 tcgaagccct gcgctcggtg ccgctcttcg ccacgctcga cgatgaacgg gccggcgact
      481 tgcgcgagct gctcgacgtg cgccacgcct cggccggcga gcgcctgttc cgcgccggaa
      541 gcatgggcga cgcgatgtac ttcatcgagg acggccgcgt ccgcatccat ctgcgcgacg
      601 aggacggcga cgacgtgacc ttagccgagc tggcccgcgg cgacttcttc ggcgagatgt
      661 ccatcctcga cggcaagccg cgctcggccg acgccaccgc cgcgtcggcc gcccgcctcg
      721 ccgtcctcac ccgcgacgac tttctacact tcgtgcgcgc caaccctgac gtggccctcg
      781 ggatgctctc cgccattgcc gagcgcctgc gccagacgga cgaactgctg cgccgccgcg
      841 tcacgcgcaa cgtcaacgag gagtacgagg agcggcgcac ggccgccgac cgcatggccg
      901 acgccgtctc ctccttcggc ggcagttgga ctttcgtcgg ggccacggcg ctcttcgtcg
      961 ccggctgggt cttcgtcaac tcgtgggtgc aggcggagcg cggcatcgac ccgttcccct
     1021 actccttcct cgacgtaatc aacggcatca tcgccgccct cgtcgccccc ttcatcctca
     1081 tctcgcagaa ccgccagcag gagaaagatc gcctccgctc cgacctcgac tttcaggtca
     1141 acctcaagaa cgagatggcc ctcaacgaac tcctgcgccg cctcgacgta ctggagagcg
     1201 aacgcctccc gctcctcttc cgcgagcaga acgaacgact ggagaagaca gtgagcagta
     1261 agcagtgagc agtgagcggt aagcagtaag gcgacaacca gccgcggagc ggcggcagaa
     1321 tgtagccacg ggcgccagcc cgtggaatag tcgtcaataa aaatctaaag ccccggaggg
     1381 gcgaaagaaa tgcgacgggc cttgtgctca acgtcctgat tctttcgccc ctccggggct
     1441 ttcggtttct ttgcctacgt tgacccacgg cttgcgccgt gggctacaat ctgccgccgc
     1501 tccgcggctg gatgactgct cactgcttac cgctactgct cactgctgct acaatgctcg
     1561 cgctccaccc gaaaatattt acgacgtgaa ggctacatga ttcaggccgt tctttttgat
     1621 tttaacggcg tcgtcatcga cgacgagccg ttgcagatga aagcatttca ggaagtcctg
     1681 cggggcgagg gaatgagcct cacggaggag gagtattacg gctcgctcgg catggacgac
     1741 gcgaccttcg tgcgcgccgc cttcgagcgc gcgggccgcg aggcggacgc ggcgaagctt
     1801 gaagagttca tcgaccgcaa gacggagatt caccgcgcgc tgatgaagga cgagctgccg
     1861 atcttccccg gcgtcgtcaa ctttatcaag gcgctgcggc accgctactc gctcggcctc
     1921 gtcagcatgg cgcgccgctc cgagatcgac tacgcgctcg aacgcgccgg cctccagaac
     1981 gccttcgagg tgatggtgag cgccgaggac gtacaggtct gcaagcccga ccccgcctgc
     2041 tacaaccgcg cgctcacact cttaaatgag acgcgccgaa gccgcgccca cgtgctgccg
     2101 ctccggcccg ccgaatgcct cgccatcgag gactcgccgc ccggcgtcag cgccgcccgc
     2161 gccgccggca tgcgtaccct cgccgtcacc aacaccgtcg ccgagcgact cctgcgcgag
     2221 gccggcgccg aaatcgtcac ccacagtctc gccgactgga cgaccgacgc cgtccaccac
     2281 gtctatgaca agcgatgaga aggcaggagt caggagtcag aagtcagagt aaaagcgttt
     2341 gccagcgtcg ctgcttcccg tcatggcttc gagcttttgc acacgccttc tgattcctgc
     2401 ttctgacttc tgacttctga cttcctacgt cttatgactc cagccgacaa agtagtcgcg
     2461 gcgagcgcgc ggagccggtt cgcgctggag gtgttgcggc gcggcgagga gattaatctg
     2521 gcgcgcgcgg cgctgctcgt cgcggccgag gaggacggga gttgcgacgt ggaggcgtcg
     2581 ttggaaacac ttgcgcggat ggggcgcgag gcgcgcgagc gcgtggaggg cgagcgcggc
     2641 ggcgcgccca tcgttgcgct caaccgctac gtgttcgagg agttgggatt tgcgggcaac
     2701 cgcgagaagt attacgaccc gcgcaacagt ctgctcaacc gcgtcgtcga gcgccggacg
     2761 ggcatcccca tcacactctc gattgtctac atggagctgg gtcggctcgc cgggctggag
     2821 gtcgagggcg tcgggatgcc gggccacttc atcgtgcgcg cgcgcggcgt tgagggcgcg
     2881 cccgttctcg tcgatccgtt caaccgggag atcgttgacg aggaggactg ccagcaccgc
     2941 ctcgacgaag tctatggcgg gcaggcgccg ctcaccgcgg agcacctgcg ctccgtctcc
     3001 aagcgcgaga ttctcatccg cctgctaacg aacctgaagt cggtctacgc gcaggggcag
     3061 ctctaccggc gggcgctggc cacggtcgag cgcatccttt tgctcgcgcc ccacgcgctc
     3121 tcggagcggc gcgaccgcgg cctgctgctc gcgcaactcg aacgcttgcc cgaagccgtc
     3181 gccgacgtgc aggcatatct ccggctcaac gagaacgccc ccgacgtgga gtcactgcgc
     3241 gagcagttga aaaagattca ggcccggcag gcgatgct
//
LOCUS       DASVES010000180         5984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_652514, whole genome shotgun sequence.
ACCESSION   DASVES010000180 DASVES010000000
VERSION     DASVES010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652514"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1172
                     /locus_tag="VF240_07300"
     CDS             222..1172
                     /locus_tag="VF240_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7175072.1"
                     /translation="MQFPFTLPTDREFDAVGFGLNAVDHLIVVPAYPEFDTKTRLVTH
                     QQSAGGQTASAMVALRRLGMQTAYAGRFGSDDEGAFGLRALEDEGVNVEYAQVIDGAR
                     NQIAFIVIDARTGERTIIWDRDERLAYSDEDAPAGFGPRGRVLHLDAHDPAACARVAR
                     EARAAGAVVSADIDNIYDGLPELLPLIDILITSKEFPRRLTGLTDGREALREVKARYG
                     CAVVGMTLGEGGAVLYHEGRFVESAAFPVPGGCRDTTGAGDAFHAGFIYGMLQGEEIE
                     ACMTLGCAVAALKCRALGARTSLPSVHELNGLLTSEGETS"
     gene            1242..1673
                     /locus_tag="VF240_07305"
     CDS             1242..1673
                     /locus_tag="VF240_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175073.1"
                     /translation="MSEQPEDNNLLNFIAATVETMRDQMATKSDLEPLRDQVEYLGGQ
                     VESMRDRMATKEDLAGVATKADLTSLEARLTDKLEVEIIAVRGDIEQIHLRLDSIDRA
                     LSARLGQIETEMSRLRSVVYLLAKDRPELLRLLGQTPPPGV"
     gene            complement(1731..3104)
                     /locus_tag="VF240_07310"
     CDS             complement(1731..3104)
                     /locus_tag="VF240_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7175074.1"
                     /translation="MMRPIRFSCVVVLLFLSAVTVSAQRRGGGGAGVNNWPSFRGEQA
                     AGVAEGQNLPERWDAAKGLGLKWKTRIPGLSHASPVVWGEHVYVTTAVSSRGLDRFRH
                     GLYGDGDAAEDRSPHQWKLYALERGTGKILWERTAYEGAPREKRHIKATYANSTPATD
                     GKHVVAFFGSQGLYAYDTKGTLLWKKDLGVLDAGAYDAPDYEWGTASSPIIYKNLVIL
                     QVDTQKEDFLVAYDVKTGREVWKATRDELPSWGTPNVYTGRGRAELVTNASNFIRGYD
                     PATGKELWRLGGSSKITAPTPVFSDGLIVVASGRRPEAPIFVLRPGASGDITLGADQS
                     ANAHVVWSKQARGSYMPTPLVYGPHLYVLNNSGLFDCYDLKTGAEVYRERIPHQGGGF
                     SASPVAADGKIYLTAEDGDIFVVRAGPKFELLATNPVGELLMATPAISSGTMFVRAHR
                     HLYAIGR"
     gene            complement(3113..3658)
                     /locus_tag="VF240_07315"
     CDS             complement(3113..3658)
                     /locus_tag="VF240_07315"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7175075.1"
                     /translation="MHLTIGELLDYTDEERAKWRRWFDEHGGEPLKTPLAGETHTSVG
                     ALILHVFWAEMFYARWMRGETLTEDSAVVTENKDLPNDDVGKLFDFGLRARDEMRAFA
                     DAASREEWEQMCEVESDGFHLRGSARKLVVHILVHEVRHWAQVAVVVRQDGLAPPGDH
                     DLCFSESFGPLLKRVGGGGEE"
     gene            complement(3756..4439)
                     /locus_tag="VF240_07320"
     CDS             complement(3756..4439)
                     /locus_tag="VF240_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX7175076.1"
                     /translation="MTILDEAEVRVLGALIEKQIATPEYYPLTLNALTAACNQKNNRH
                     PVVSYDEATVELAVESLRQKNLAYVFYGAGSRVPKYKHVAAENLQLKAAELALACVLM
                     LRGPQTLGELRDRSARLYEFPGLEEVEAALGSLATREEPVVTRLARRPGQKEARYAHL
                     LSGEVFEQHEEESSETAAAAASPSHARTTTTTTSAERVLKLEQEVETLRAELDGFRRE
                     FETFKKQFE"
     gene            complement(4550..5536)
                     /gene="galE"
                     /locus_tag="VF240_07325"
     CDS             complement(4550..5536)
                     /gene="galE"
                     /locus_tag="VF240_07325"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX7175077.1"
                     /translation="MRIVVTGGAGYIGSVVTEELIKDGHEVAVYDNLSKGHRAAVHPE
                     ARFVHADLFDAEVLRRTFREQQTEAVVHMAADALVGESVSDPAKYYRNNFVGGLQLLD
                     AMRDCGVARIVFSSTCATYGEPERLPMDETLRQQPTNPYGQSKLAFEGALKWYGAAYG
                     ISYASLRYFNAAGATERFGEQHDPETHLIPIVLQAAAGKRPHVEIYGADYPTRDGTCV
                     RDYIHIVDLARAHILALSAPAGGQSGAAYNLGCGGDGYTVKELIDVAREVTGRDIPVR
                     VGVRRAGDPAVLVATSEKIKRELGWEPQFQDLRKIIESAWVWAQAHPNGYES"
     gene            5677..>5984
                     /locus_tag="VF240_07330"
     CDS             5677..>5984
                     /locus_tag="VF240_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175078.1"
                     /translation="MPSVSAQADTTETGAPEERPAVSLWRQPDFLKFWLGMNINLLGS
                     HFGGLAYSLTAVVLLNASPMQMGLLSATRTAASFLVGPFAGVLLDRTRRRPVILGSDL
                     A"
BASE COUNT          971 a         1999 c         1965 g         1049 t
ORIGIN      
        1 ggaattgcaa gcggggctga ttcctctcgt ggcggccgct tcagcgccga cgcggcgtga
       61 cgacttcgtt gaagcttcct cggttcttga gagcagggcg accggcattc accgcagagg
      121 cgcagagaac gcccgtcgcc ctgccctcaa gaaccgagaa tgaagacagg cagccggccc
      181 gccgccgaag gagccgccag cccaaaagga atcaagtccg cttgcaattc cctttcacac
      241 ttccaaccga cagagagttc gacgccgtcg gcttcggcct caacgccgtc gatcacctca
      301 tcgtcgtccc cgcctacccc gagttcgaca ccaagacgcg gctcgtcaca caccagcaat
      361 cggccggcgg gcagacggcc tccgcgatgg tcgcgctccg acgactcggc atgcaaacgg
      421 cctatgccgg ccgcttcggc tctgacgacg agggcgcttt cggcttgcgc gcgctcgaag
      481 acgaaggcgt caacgtcgag tatgcacagg tgattgacgg ggcgcgcaat cagatcgcct
      541 tcatcgtcat cgacgcgcgc accggcgagc gcacgatcat ctgggacagg gacgaacgcc
      601 tcgcctacag cgacgaagac gcgcccgctg gctttggccc ccgaggtcgc gtcctccacc
      661 tcgacgccca cgaccccgcg gcctgcgccc gcgtcgcccg cgaggcgcgc gcggccggcg
      721 ccgtcgtctc cgccgacatc gacaacatct acgacggcct gccggagctg ctgccgctca
      781 tcgacattct catcacgtcg aaagagtttc cccgccgtct gacggggcta acggacgggc
      841 gcgaggcttt gcgggaagtg aaggcgcgct acggttgcgc ggttgtcggg atgactctcg
      901 gcgagggggg cgcggtgctt tatcacgagg gccgcttcgt cgagtcggcg gccttccccg
      961 tccccggcgg ctgccgcgac acgacgggcg cgggcgacgc cttccacgcc ggcttcatct
     1021 acgggatgct ccagggcgaa gagatcgagg cgtgcatgac gctcggctgc gccgtcgccg
     1081 cgctcaagtg ccgcgctctc ggcgcgcgca cgtcgctccc gtcggtgcat gagttgaacg
     1141 gcttgctaac ttcggagggc gagacttcct gaagggccgc gcggccggtt ctattccggc
     1201 taataatccc gtcgcgcgtg tgctacgata agaccaacgc catgagcgaa cagcccgaag
     1261 acaacaacct gctcaacttc atcgccgcca ccgtcgagac gatgcgcgac cagatggcga
     1321 ccaagagcga ccttgagcct ctacgtgatc aggtcgaata cttgggtggc caagttgagt
     1381 cgatgcgcga ccggatggcg acgaaggaag acctcgccgg cgtggcgacg aaggcagacc
     1441 tcaccagcct ggaggcccgc ttaacggata agctggaagt tgaaatcatc gccgtccgcg
     1501 gcgacatcga gcagatacac ttgcgtctgg attccatcga ccgcgccctc tccgcgcgtc
     1561 tcgggcagat cgagacggag atgagccggc tgcgtagcgt cgtctacctg ctcgccaagg
     1621 acaggcccga actgctgcgg ctcctcggtc agacaccccc tccgggcgtc tagcccgccc
     1681 ttaagaaact cgctactcca acccgcgcac ttgaatgtgt ttgatttccc tcagcgcccg
     1741 atggcgtaca ggtgtcggtg cgcgcggacg aacatcgtgc ctgaggagat ggcgggggtg
     1801 gccatgagca gttcgccgac ggggttggtc gccagcagct cgaacttggg gccggcgcgg
     1861 acgacgaaga tgtcgccgtc ctcggccgtc agataaatct tgccgtcggc ggcgacgggc
     1921 gaggcgctga agccgccgcc ctgatgcggg atgcgctcgc ggtagacttc cgcgccggtc
     1981 ttcaaatcat aacagtcgaa gaggccggag ttgttgagca cgtagaggtg tgggccatag
     2041 acgagcggcg tcggcatgta ggagccgcgc gcctgcttgc tccagacgac gtgcgcgttg
     2101 gcgctctggt cggcgccgag cgtgatgtcg ccggaggcgc cggggcgcag cacgaagatg
     2161 ggggcttcgg ggcgacggcc cgaggcgacg acgatgaggc cgtccgagaa gacgggcgtc
     2221 ggcgcggtga tcttggagct gccgcccaag cgccacagtt ccttgcccgt ggcggggtcg
     2281 tagccgcgga tgaagttcga ggcgttggtg acgagttccg cgcggccgcg tcccgtgtag
     2341 acgttcggcg tgccccacga gggcagctcg tcgcgcgtcg ccttccagac ctcgcggccg
     2401 gtcttcacgt catacgcgac gaggaagtct tccttctgcg tgtcaacttg gaggatgacg
     2461 aggttcttat agatgatggg cgagctggcc gtgccccact cgtaatcggg cgcgtcgtag
     2521 gcgccggcgt ctaagacgcc gaggtctttc ttccagagga gcgtgccctt cgtgtcatag
     2581 gcgtagaggc cctgcgagcc gaagaaggcg acgacgtgtt tgccgtcggt cgcgggcgtg
     2641 gagttggcgt aggtggcctt gatgtggcgc ttctcgcgcg gtgcgccctc gtaggccgtg
     2701 cgctcccaga gaattttgcc ggtgccgcgc tccaaggcgt agagcttcca ctggtgcggg
     2761 gagcgatcct cggcggcgtc gccgtcgccg tagagaccgt gccggaagcg gtcgaggccg
     2821 cggctgctga cggccgtcgt cacgtagacg tgctcgcccc agacgacggg gctggcgtgc
     2881 gacagtcccg gaatcctcgt cttccatttt agccctaagc ccttcgccgc gtcccaacgc
     2941 tccggcaaat tctgcccctc ggcgacgccc gcagcttgct caccccgaaa cgacggccag
     3001 ttgttcacgc ccgcgccgcc gccgccgcgc ctctgcgccg agacggtgac ggccgacaag
     3061 aagagcagca caacgacgca cgaaaaccgg atcggtctca tcattcgtct catcattctt
     3121 cacctccgcc cccgactctt ttaagcagcg ggccgaacga ctcgctgaag cacaggtcgt
     3181 gatcgcccgg cggggcgagt ccgtcctgcc ggacgacgac ggccacctgc gcccagtgcc
     3241 tgacctcgtg cacgaggatg tgcacgacga gcttgcgcgc cgacccgcgc agatgaaagc
     3301 cgtccgactc gacctcgcac atctgttccc actcctcacg gctcgccgcg tcggcgaagg
     3361 cgcgcatctc gtcgcgcgcg cgcagtccga agtcgaacag cttcccaacg tcgtcgttcg
     3421 gcaaatcctt gttctctgtg acgaccgcgc tgtcttccgt cagggtctcg ccgcgcatcc
     3481 agcgggcgta gaacatctcg gcccagaaga cgtggaggat gagcgcgccg acgctcgtgt
     3541 gcgtctcgcc ggccagcggc gtcttcagcg gctcgcctcc gtgctcgtcg aaccagcgcc
     3601 gccacttcgc gcgctcctcg tccgtgtagt cgagcagctc tccgatagtc aggtgcatcg
     3661 cgtcccgtcc tctcaaaccc tgcggcgtga actgctgcgt cctgcggcga ctccgcggtg
     3721 agttgattgt cgtaacactc aaccgaagag tcatcctact caaactgttt cttaaacgtc
     3781 tcgaactcgc gccggaaacc atccagctcc gcgcgcaacg tctcgacctc ctgctccagc
     3841 ttcaggacgc gctcggctga tgtcgtcgtc gtcgtcgtgc gcgcgtgcga cggcgacgcc
     3901 gcggcggccg ccgtctccga cgactcctct tcgtgctgct cgaacacctc gccggagagc
     3961 aggtgtgcgt agcgcgcctc cttctgtccg ggccggcgcg cgagacgagt gacgaccggc
     4021 tcctcgcgcg tggccagcga acccagcgcc gcctcgacct cttcgagtcc ggggaactcg
     4081 tagaggcgtg cggagcggtc gcgcagctcg ccgagcgtct gcggcccgcg cagcatgagg
     4141 acgcaggcta aggccagctc cgccgctttg agttgcagat tctccgccgc gacgtgcttg
     4201 tacttcggca cgcggctgcc cgcgccgtag aagacgtagg cgaggttctt ctgacgcaaa
     4261 gattcgacgg ccagctcgac cgtcgcctcg tcgtaggaga cgacggggtg acggttgttc
     4321 ttctgattgc aggcggccgt caacgcgttc agggtcagcg ggtagtactc gggcgtcgcg
     4381 atctgcttct cgatcagcgc gccgagaacc ctgacttccg cctcgtcaag tatggtcatt
     4441 ggttcaaaag cggtgatgag tgatgagtga taagtgataa gagaagacaa agcttgcctt
     4501 ttctttctta tcacttatca ctcatcactc atcaccctct cgtcaccttc taagactcgt
     4561 acccattcgg gtgcgcctgc gcccagaccc aggccgactc gatgatcttc ctcaagtcct
     4621 gaaactgcgg ctcccaccca agctcgcgct tgatcttctc ggaggtggcg acgaggacgg
     4681 ccgggtcgcc cgcgcgtcgc acgcccacgc gcacggggat gtcgcggccc gtcacctcgc
     4741 gcgccacgtc gatgagttct ttaacggtgt agccgtcgcc gccacagccg aggttgtagg
     4801 cagcgcctga ttgtccgccc gccggcgcgg agagcgcgag gatgtgcgcg cgcgccagat
     4861 cgacgatgtg gatgtaatcg cgcacgcagg tgccgtcgcg cgtcgggtag tcggcgccgt
     4921 agatttcgac gtgcgggcgc ttgccggcgg cggcctgaag cacaatcggg atgaggtgtg
     4981 tttcggggtc gtgctgctcg ccgaagcgtt cggtggcgcc ggccgcgttg aagtagcgaa
     5041 ggctcgcgta gcttatcccg taggccgcgc cgtaccactt gagcgcgccc tcgaaggcga
     5101 gcttcgactg gccgtagggg ttggtcggct gctggcggag cgtctcgtcc atcggcaggc
     5161 gctccggctc gccgtaggtg gcgcaggtcg aggagaagac gatgcgcgcg acgccgcagt
     5221 cgcgcatcgc gtcgaggagt tggaggccgc cgacgaagtt gttgcggtag tacttcgcgg
     5281 ggtctgagac ggactcgccg acgagcgcgt cggccgccat gtggacgacc gcctcggtct
     5341 gctgctcgcg gaaggttcgg cgcagcactt ccgcgtcgaa gaggtcggcg tggacgaagc
     5401 gcgcctcggg gtggacggcc gcgcggtggc ccttcgagag gttgtcgtag accgcgacct
     5461 cgtggccgtc tttgatcaac tcttcggtga cgacgctgcc gatgtagccg gcgccgcccg
     5521 tgacgactat ccgcatctgt tgtttccatt tcgccggcgc ggcgcgcggc gtccaacatg
     5581 aagttactga ccgggcaagc cgggccattc tagcacgcac gccgaagacg gtctaaagag
     5641 tgccgcgccg ggaacacgag ggagcgagag cgcgtagtgc cttccgtgag cgcacaggcc
     5701 gacacgacgg agacgggagc gccggaggaa cgtccggcgg tgagcctgtg gcgccagccc
     5761 gacttcctca aattctggct ggggatgaac atcaatctcc tcggcagtca cttcggaggg
     5821 ctggcctact cgctcacggc cgtcgtcctc ctcaacgcga gccccatgca gatgggcctg
     5881 ctcagcgcga cgcgcacggc cgcctccttc ctcgtcggcc ccttcgccgg cgtgctgctc
     5941 gaccgcacgc gccgacgccc cgtgattctc ggctccgacc tcgc
//
LOCUS       DASVES010000181         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_184154, whole genome shotgun sequence.
ACCESSION   DASVES010000181 DASVES010000000
VERSION     DASVES010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184154"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1123
                     /locus_tag="VF240_07335"
     CDS             182..1123
                     /locus_tag="VF240_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7175079.1"
                     /translation="MMNRRHFLATLSAGLAASGVGLADTGKDSKPQVAITIDDIAASA
                     SFDVSAAERHRRLREALAGRSKLKAAIFVRGKLVDSDEGRQLLRAWDDDGHLIGNHTY
                     SHQYYHSQKLTFEEFSADVLRGEAVIKDLKNFRRLFRFPYLKEGDTLPKRDRMRALLK
                     RHGYRMGHVTIDASDWYIAQRMTERLKQNPKADLTGYRDFYLAHMWERSAFYDDLARR
                     VALRPVRHTVLLHDNLLNALFLADLLRMYEEKGWRLIDAGKAFTDPLFRAEPNILPAG
                     ESIVWALAKESKKFEHLLRYPAEDGPYEKEKMDRLGL"
     gene            1295..4333
                     /gene="treY"
                     /locus_tag="VF240_07340"
     CDS             1295..4333
                     /gene="treY"
                     /locus_tag="VF240_07340"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX7175080.1"
                     /translation="MDDQKTVPARIPVSTYRLQFNSRFTFEQARAAVAYLDELGVGCC
                     YSSPVLKARPGSLHGYDVVDHSRLNPEVGAEEQFAEFAGELRRRGMGLLMDAVPNHMC
                     IASAENRWWMDLLEDGPGSPYARFFDVDWNPPNPSLKNKVLLPILGDQFGRVLENQEI
                     KITRRDGAFFANYYETTLPIAPRTATHILEAALRSLAARLTEAHADVLELESVITALG
                     HLPPRTETDPARVRERRREREVIRRRLSSLLKQGGEARRAVDEAIDEINGVRGRPETF
                     DRLERLLAEQSYRLSYWRVAADEINYRRFFDINELAAVRVEERPVFTAVHDRLLRLMA
                     RGLVTGLRIDHVDGLFNPEKYLHDLRRECAALAARGVATGDAPAESADSVSRGDSVPA
                     EHGEAERREGERSSVEQPESGRQGPACYVVVEKILGHDELLRREWPVHGTTGYDFLNL
                     LGGLFVDGRGAQAFRELYAEFTGARTNFADLVYACKKLVLRVAMSSELHVLARRLARI
                     SERLRSSRDFTFNSLHDALGEVVACFPVYRTYIRRRQDLASADDRLHINSAVASAKLR
                     NPSLSGSIFDFIASILMLDDPKGTTPAQRAERRDFVMRFQQLTSPIMAKGLEDTAFYR
                     YYPLASLCEVGGEPAIFGTPLARFHRRNLYRQEQWPHALSATSTHDTKRGEDVRARIH
                     ALSEMPEEWNRAVHRWREMNRARRTQLDGVAAPDANEEYLFYQTLVGAWPFAPMNREE
                     HAAFVERISEYMRKALREAKLHTSWINQNEEYERAVDEFVRASLDGAEGNSFLADFVE
                     FQQATARAGVYNSLSQTLLKIVSPGVPDFYQGTELWQLDLVDPDNRRPVDYAHRRALL
                     ASLDAGEAGDDRAAFVESLLREPEDGRVKLYVTSRALRFRRAHPDLFAHGEYVPLAAS
                     GEAADSVVAFARKLGDREVLAVAGRFFTRLANRQTPRLPLGVEAWGDTSLALGEKLSA
                     VRFRDVFTGVEVNPTEGQGAGSLDVPAIFSRLPVALLERV"
     gene            4350..>4643
                     /locus_tag="VF240_07345"
     CDS             4350..>4643
                     /locus_tag="VF240_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175081.1"
                     /translation="MTNERPVKVWPGQPYPLGATWDGAGVNFALFSENATAVELCLFD
                     AADAAEESARVRLTEYTDQVWHAYLPEVRPRQLYGYRVHGPYDPRRGQRFNPHK"
BASE COUNT          808 a         1678 c         1431 g          726 t
ORIGIN      
        1 accgcagagg cgcagaggac gcagagttca cgcagagggt tgacgcttac actcaacgct
       61 gagtgaatcg ctcaagcctc tgcgcttcct ctgcgtcctc tgcgcctctg cggtgagtta
      121 tgattgcttt aactcaagta aggagccacg tagtcggccg ctcgacggtt acaatctaaa
      181 gatgatgaat cgaagacact tcctcgcaac cctcagcgct ggcctcgccg cgtccggcgt
      241 cggtcttgca gacacaggta aggactccaa gccgcaggtc gccatcacga ttgacgacat
      301 cgccgcgagc gcctcgttcg acgtgtccgc cgcggagcga caccgacgcc tgcgcgaggc
      361 gctggccggg cgctcgaagc tcaaggcggc catcttcgtc cgcggcaagc tcgtggacag
      421 cgacgagggc cggcaactgc tccgcgcctg ggacgacgac ggacacctca tcggcaacca
      481 cacctactcg caccagtact atcactcgca gaagctcacc ttcgaggagt tctcggcgga
      541 cgtgctgcgc ggcgaggccg tcatcaaaga cctcaaaaac ttccgtcgcc tcttccgctt
      601 tccctatctc aaggagggcg acactctgcc caagcgcgac cggatgcgcg ccctcctcaa
      661 gcgccacggc taccgcatgg gccacgtcac catcgacgcc tccgactggt acatcgcgca
      721 gcgcatgacc gagcgcctca agcagaaccc gaaggccgac ctcacgggct acagggattt
      781 ctacctcgcg cacatgtggg agcgttcggc cttctacgac gacctcgccc gacgcgtcgc
      841 gctccgcccc gtccgccaca ccgtgctcct ccacgacaac ctgctcaacg cgctcttcct
      901 cgccgacctt ctccgcatgt acgaggagaa aggctggcgc ttgatcgacg ccgggaaggc
      961 gttcaccgac cccctctttc gcgccgagcc gaacatcctg ccggccggcg agagcatcgt
     1021 ctgggcgctc gccaaagagt cgaagaagtt cgagcacctg ctgcgctacc ccgccgagga
     1081 cggcccctac gagaaagaga agatggatcg gctcggtctg tgacggccgg ttcgagtttc
     1141 ctcgtcggtt gagccaacag gtataccgct tctaaaagtt tagtaaacag atgtgctgag
     1201 cgtctcgcct cagcggcgcg cacaaaaaga aaagaaatct tcggcccctc ggataaagcg
     1261 cgaacgcggg cggttggttt aagatgtggt cggaatggac gaccagaaga ccgtgccggc
     1321 gcgcatcccc gtctccacct accggctaca gttcaactcc cgattcacct tcgagcaggc
     1381 gagggcggcc gtcgcctacc tcgacgaact cggcgtcggc tgctgctact cttcgccggt
     1441 cttgaaggcg cggcccggca gcctgcacgg ctacgacgtg gtcgatcact cccggctcaa
     1501 cccggaggtc ggcgccgagg agcagtttgc ggagttcgcc ggagagctgc gaaggcgcgg
     1561 gatgggcctg ctgatggacg cggtgcccaa ccacatgtgc atcgcctcag cggagaaccg
     1621 ctggtggatg gacttgctcg aagacggccc cggctcgccc tacgcgcgct tcttcgacgt
     1681 ggactggaac ccgccgaacc cgtcgctcaa aaacaaagtg ctcctgccca tcctcggcga
     1741 ccagttcggg cgcgtcctcg aaaatcagga aattaaaatc acccgccgcg acggcgcctt
     1801 cttcgccaac tactacgaga ccacgctccc catcgccccg cgcaccgcca cgcacatcct
     1861 cgaagcggcc ctgcgctcgc tcgccgcccg actcaccgaa gcgcacgccg acgtgctcga
     1921 actcgaaagc gtcatcacgg ccctcggcca cctgccgccg cgcacggaga ccgacccggc
     1981 ccgcgtgcgc gagcgacggc gcgagcggga ggtcattcgc cggcgtctgt cgagtttgtt
     2041 gaagcagggc ggcgaagcgc gccgcgccgt ggacgaagcc attgatgaga tcaacggcgt
     2101 gcgcgggcgg cccgagacct tcgaccggct ggagcgcctg ctcgcggagc agtcgtaccg
     2161 cctgagctac tggcgcgtcg ccgccgacga gatcaactac cgccgcttct tcgacatcaa
     2221 cgagctggcc gccgtccgcg tcgaggagcg gcccgtcttc accgccgtcc acgaccgcct
     2281 gctgcgcctg atggcgcgcg gcctcgtcac cggattacgc atcgaccacg ttgacggcct
     2341 cttcaacccg gaaaaatatc tgcacgacct gcgccgcgag tgcgccgcgc tcgccgcgcg
     2401 cggagtggcg accggcgacg cgcccgcaga gagcgccgat tccgtcagtc gcggcgactc
     2461 cgtgccggcc gagcacgggg aagctgagcg gcgcgagggt gagcggtcga gcgtcgagca
     2521 accggagagc gggcggcagg ggccggcgtg ctacgtcgtc gtcgagaaga ttctcggaca
     2581 cgacgagctg ctgcggcgcg agtggcccgt ccacgggacg accggctacg acttcctgaa
     2641 tcttctgggc ggcctcttcg tcgatgggcg cggcgcgcag gcgttccgcg agctgtacgc
     2701 cgagttcacc ggcgcacgca cgaacttcgc cgacctcgtc tacgcctgca agaagctcgt
     2761 cctgcgcgtg gccatgtcga gcgagctgca cgtccttgct cgacgcctcg cgcgcatcag
     2821 cgagcgcctg cgttcctcgc gcgacttcac cttcaacagc ctccacgacg ccctcggcga
     2881 agtggtcgcc tgcttccccg tctaccgcac ctacatccgc cgccggcagg acttggcgag
     2941 cgccgacgac cgactccaca tcaactcggc cgtcgcctcc gccaagctcc gcaacccatc
     3001 gctcagcggc tccatcttcg acttcatcgc ctccatcctc atgctcgacg acccgaaggg
     3061 gacgacgccc gcgcagcgcg ccgagcggcg cgacttcgtg atgcgcttcc agcagttgac
     3121 cagccccatc atggcgaagg gcctcgaaga cacggccttc taccgctact atccgctcgc
     3181 ctcgctgtgc gaagtcggcg gcgagccggc catcttcggc actccgctcg cgcgcttcca
     3241 ccgacgcaac ctttaccggc aggagcagtg gccgcacgcg ctctcggcca cctcgacgca
     3301 cgacaccaag cgcggcgagg acgtgcgcgc gcgcatccac gcgctctccg agatgcccga
     3361 ggagtggaac cgcgccgtcc accgctggcg cgagatgaac cgcgcccgca ggacgcagct
     3421 cgacggcgtg gccgcccccg acgccaacga ggaatatctc ttctaccaga cgctcgtcgg
     3481 cgcgtggccc ttcgcgccga tgaacagaga ggagcacgcc gcgttcgtcg agcgtatcag
     3541 cgagtacatg cgtaaggccc tgcgcgaggc gaagctccac acgagctgga tcaaccagaa
     3601 cgaggagtat gagcgggccg tggacgagtt cgtgcgcgcg tcgctcgacg gggccgaggg
     3661 caacagcttc ctcgcggact tcgtcgagtt tcaacaggcg acggcgcgcg ccggcgtcta
     3721 caactcgctg tcgcagacgc tcctcaagat cgtctcgccg ggcgtgcccg acttctatca
     3781 gggcaccgaa ctctggcagc tcgatctggt tgacccggac aaccgccgcc ccgtcgatta
     3841 cgcgcaccgc cgcgcgctcc tcgcctcgct cgacgcgggc gaagcgggcg acgaccgcgc
     3901 cgccttcgtc gaaagtctcc tgcgcgagcc ggaggacggc cgcgtcaagc tctacgtgac
     3961 gagccgcgcg ctccgcttcc gccgcgcaca tcccgacctc ttcgcgcacg gcgagtacgt
     4021 gccgctcgcc gcgtcgggcg aggcggccga tagcgtcgtc gccttcgcgc gcaaactcgg
     4081 cgacagggaa gtgctcgccg tcgcgggccg tttcttcacg cgcctcgcga accggcaaac
     4141 gccccgtctg ccgctcggcg tcgaggcgtg gggcgacacg tcgcttgcgc tgggcgagaa
     4201 actgtcggcc gtgcgcttcc gcgacgtgtt cacgggtgtt gaagtaaatc cgactgaggg
     4261 acagggggcc ggctcgctcg atgtgccggc gatttttagc cggctcccgg tcgcgcttct
     4321 ggaacgagtt tgagactgtc tcgacgatta tgacgaacga aagacctgtg aaagtctggc
     4381 ccggccagcc ctacccgctc ggcgccacgt gggacggcgc cggcgtcaac ttcgccctct
     4441 tctcggagaa cgccacggcc gtcgagctgt gtctcttcga cgcggcggac gccgccgaag
     4501 aatccgcgcg cgtccggctc accgagtaca ccgatcaggt gtggcacgcc tacctgcccg
     4561 aggtgcggcc ccgacagctc tacggctacc gcgtccacgg cccctacgac ccgcgccggg
     4621 gccaacgctt caacccgcac aag
//
LOCUS       DASVES010000182         6094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_200340, whole genome shotgun sequence.
ACCESSION   DASVES010000182 DASVES010000000
VERSION     DASVES010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200340"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VF240_07350"
     CDS             <1..903
                     /locus_tag="VF240_07350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7175082.1"
                     /translation="PPLLFAGAAMLLIGFGFKIATAPFHVWTPDVYEGAPTPVTAFMA
                     AGPKAAGFASFMRVFLFAFPFVAGGLAESVHDAWLGALWLLAILTMTVGNVVALVQTN
                     VKRMLAYSSIAHAGYALVGFVAAGAARGDAERQSAVAAVIFYLLAYAVMNLGAFAVVT
                     LIARSGDRRTDVDDYRGIGFSTPWLAFALTVFLLSLLGIPLTAGFMGKIMVFREAVDQ
                     GYIGLVVIGVLNTAVSVYYYLRLIVLMFFRDRGVEWEAPRVPASIAVTLVLTLAGVFY
                     LGLFPGRVLERLRAPRPAAVSRAK"
     gene            1223..1798
                     /locus_tag="VF240_07355"
     CDS             1223..1798
                     /locus_tag="VF240_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175083.1"
                     /translation="MPTAGASTLKTKSVAEDSGRGRAARPSADSITARVREELGEAWV
                     PRLYRERILQTRTRSHTVFPAAKGAVVEVQHTLLGIELKVGRRRQSCPDLSTARYLAV
                     FARAGCSEVAVPYDITRISHLADELESAWQRMLLLAAHHTEGRARSFASHVRSMLAAE
                     VRREVEEAGPGAAVPEFKQSTRQRRTPVQEG"
     gene            1825..2856
                     /locus_tag="VF240_07360"
     CDS             1825..2856
                     /locus_tag="VF240_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175084.1"
                     /translation="MQEFNFNEPPRPDHQEPLPFPFNQRPFCRYENIEKRVAAVRVLV
                     VNSLLRWETDVSDVARAELGAKLARQLERERYISELAANNILGNIRRLVERPETEVVH
                     LSEVAEAAERFRPDAVVLSGTLSDFDYYNPSVIDDFGRYIRTTRTPVLGICGGHQLVG
                     MCFGARVVTLSDGREPAERRAGRAFEYQYRFVKITDPDDPIFRGVKDPESGVWQDYTV
                     EGRTLRVWQNHGLQLDRVPEGFRMLATSYLCRNQMMVRRAEGQLIYAVQFHLEKSFQD
                     LSRGYERRLNPNLAREVRKRLLREEAESRTRWEHQNESRDGRAIFENFLREALQHVAA
                     SGAEANAQT"
     gene            complement(2956..4098)
                     /locus_tag="VF240_07365"
     CDS             complement(2956..4098)
                     /locus_tag="VF240_07365"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX7175085.1"
                     /translation="MFEKYTLGIEEEFQIVDPQTRLLVSHVSEILEEGKMILGEQVKP
                     EMMQAQVEVGTGICRNIEEARADITRLRAIVSHLARKKGLAIIAASTHPISQWTDQKV
                     FDDPHYALLIEELQMVARSLLIFGLHVHVGIEDRERQIHIMNASRYFLPHMLALSTSS
                     PFWLGINTGLKSYRSEVFKKFPRTDIPDHFDSYQSYERYVELLKKTNCISNPKKIWWD
                     IRPHPTFPTLEFRACDIPTRVDDTLALAALFQAIVCKLDRLIERNLGFRLYRRMLIQE
                     NKWRAVRYGIGGKMIDFGKQTEVPVRDLIMELLEFVDDVVDDLGSRKEITHVHTILER
                     GTSAEEQLRVFKETGDLNAVVDRLIELTMENVPTDLSASQMAPLMG"
     gene            complement(4208..4531)
                     /locus_tag="VF240_07370"
     CDS             complement(4208..4531)
                     /locus_tag="VF240_07370"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7175086.1"
                     /translation="MDYIDSRSPQDERREFERSRLIIDVFFEGVDLTGVASSKDISPG
                     GLYMNTQADIPEGALLTVRVPFPEGDVVVNAEAVYSNPGRGVGLRFHGLTAENRTTIE
                     RALGD"
     gene            complement(4538..5890)
                     /locus_tag="VF240_07375"
     CDS             complement(4538..5890)
                     /locus_tag="VF240_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175087.1"
                     /translation="MLQEAVDYYHRLLQDEETAEGSRRMLDEGLERAKLIFGGRRLSP
                     YLRPHFVTETDFARVRRICETVWGAIQKVKDAAVEDASLLDELGITEIERDLVSIDPG
                     YRAVSPTARLDSFLIEDDYHFVELNGESPAGIAYADAAYEIFSELPVMRRFSERYNLR
                     RFHGRPLMLETLLEAHREFSGRAPEHKPTIAIVDLKGLPTQKEFELFREYFESQGYPS
                     VICSPDELEFTGGRLRGGGREIDIVYKRLLVNEYLPIMSEHPALLDAYRARAFCMVNS
                     FRSKLIHKKALFAVLTDERHAPLFNADEREVIARHVPWTRTVREGRTEYKGEGIDLVE
                     FIAENRGRLVLKPNDDYGGHGIYIGWSGETDWDEALALALSNGDYIVQERVRTARETF
                     PALHDDGSVVYAEQLVDLDPMLFNGRVGSAFTRLSSTELANVSSGGGMVPTFIIGEKG
                     "
BASE COUNT         1002 a         1999 c         1988 g         1105 t
ORIGIN      
        1 ccgccgctcc tcttcgccgg ggcggcgatg ctcctcatcg gcttcggctt caagatcgcc
       61 accgccccgt tccacgtctg gacgccggac gtgtatgagg gcgcgcccac gcccgtcacg
      121 gccttcatgg ccgcagggcc gaaggccgcc ggcttcgcct ccttcatgcg cgtctttctg
      181 ttcgccttcc ccttcgtcgc gggcggtctc gccgagagcg tccacgatgc atggctcggc
      241 gcgctctggc tcctcgcgat tttgacgatg acggtgggca acgtcgtcgc gctcgtccag
      301 acgaacgtca agcggatgct cgcctactcg tccatcgcgc acgccggtta cgcgctcgtc
      361 ggcttcgtcg cggccggcgc ggcgcgcggc gacgccgagc ggcagtcggc cgtcgccgcc
      421 gtcatcttct acctgctcgc ttatgccgtg atgaacctcg gcgccttcgc cgtcgtcacg
      481 ctcatcgccc gcagcggcga ccgtcgcaca gacgttgacg actaccgcgg catcggtttc
      541 tcgacgccat ggctggcctt cgcgctcacg gtcttcctgt taagcttact tggcatcccg
      601 ctcaccgccg gcttcatggg caagatcatg gtcttccgcg aggccgtcga tcagggctac
      661 atcggcctcg tcgtcatcgg cgtgctcaac acggccgtct ccgtttatta ctacctgcga
      721 ctcatcgtcc tgatgttctt ccgcgaccgc ggcgtggagt gggaggcgcc gcgtgtgccg
      781 gcgagcatcg cggtgacgct cgtgctcacg ctcgccggtg tcttttacct cggtctcttt
      841 ccgggacgcg tcctcgaacg gctgcgtgcg ccgcggccag cggccgtcag ccgcgcgaag
      901 tgaatcgacc gcgcgccggc ctccgcgtct aagcgtgccc cggcaggggc gcgaggaaag
      961 tagccagggg ccagcgccga agcgttagcg caggcgcgcc gcccctgagt cattcgcaca
     1021 tccaacgccg cgccctgaag gggcgcgagg aatgcgacgc gccgcgtcct cgcgcccctt
     1081 cagggcgcga acatctttta tgcgtgacca ggggctgcgc tcactcacgt tcgctggccc
     1141 ctggctactt tcccgctgcg cctccggcgc agccgcacgc ccctcgcgcc gactgacgtt
     1201 aattattgct ataatgccgc cgatgccgac agcgggcgct tcaaccctta agacgaaatc
     1261 cgtcgccgaa gattcagggc gcggccgggc ggcgcggccg tcggccgact cgatcacggc
     1321 gcgcgtccgc gaggagttgg gcgaggcgtg ggtgccgcgt ctctaccgcg agcgcatcct
     1381 ccagacgcgc acgcgctcgc acacggtttt tccggcggcg aagggggcag tggtcgaggt
     1441 gcagcacacg ctgctcggca tcgagctgaa ggtcgggcgc aggcggcagt cgtgccccga
     1501 cctctccacg gcgcgctacc tcgccgtctt cgcccgcgcc ggctgctccg aggtggccgt
     1561 cccctacgac atcacgcgca tctcgcatct cgccgacgag ctggagtccg cctggcaacg
     1621 gatgctgctt ctggccgcgc atcacaccga ggggcgcgct cgctccttcg cctcgcacgt
     1681 ccgctcgatg ctcgcggccg aagtgcggcg cgaagtggag gaggcggggc cgggcgcggc
     1741 cgtccccgag ttcaagcaga gcaccagaca gcgccgcacg cctgttcaag aagggtgagc
     1801 gcgggttaaa atatttcccg gatcatgcag gaattcaact tcaacgaacc accacggccg
     1861 gaccaccaag agccgctccc ctttcccttc aaccagcgcc ccttctgtcg ctacgagaac
     1921 atcgagaagc gcgtggcggc cgtgcgcgtg ctcgtcgtca acagtttgtt gcgctgggag
     1981 acggacgtgt cggacgtggc gcgcgccgaa ctcggcgcga agctcgcccg tcaactggaa
     2041 cgcgaaaggt acatctcgga gcttgccgca aacaacatcc tcggcaacat ccggcgcctc
     2101 gtcgagcggc ccgagacgga ggtcgttcac ctctcggaag ttgctgaggc ggccgaacgc
     2161 ttccgccccg acgccgtcgt gttgagcggg acgctcagcg acttcgacta ctacaacccc
     2221 agcgtcatcg acgacttcgg ccgctacatc cgcacgacgc gcacgcccgt gctcggcatc
     2281 tgcggcggcc accagttagt cggcatgtgc ttcggcgcgc gcgtcgtcac gctctctgac
     2341 gggcgcgagc cggcggagcg gcgcgccggc cgcgccttcg agtaccaata tcgcttcgtc
     2401 aagattaccg accctgacga ccccatcttc cgcggcgtga aagaccccga gtcgggcgtg
     2461 tggcaggact acaccgtcga ggggcgcacc ctgcgcgtct ggcaaaacca cggactgcaa
     2521 ctcgaccgcg tgcctgaagg cttccgcatg ctcgccacct cctacctgtg ccgcaaccag
     2581 atgatggtgc gacgggccga ggggcagctc atctacgccg tgcagttcca ccttgagaag
     2641 tcttttcagg acctgagccg cggttacgag cgccgcctca acccgaacct cgcgcgcgaa
     2701 gtccgcaagc gcctgctgcg cgaggaggcc gagagccgca cgcgctggga acaccagaac
     2761 gagtcgcgcg acggccgcgc catcttcgag aacttcctgc gcgaagccct ccagcacgtc
     2821 gccgcgtccg gcgccgaggc aaacgctcaa acttagcccg ctcctggaag gcaaaagtaa
     2881 aaaggcaaaa ggcaaaagtg aagaggcggc tcgcttcccc acttttgcct tttgccattt
     2941 tacttttgcc ctctcttatc ccatcagggg cgccatctgg ctggcgctca agtcggtggg
     3001 gacgttctcc atcgtcagct cgatgaggcg atcaaccacg gcattgaggt cgccggtctc
     3061 cttgaagacc cggagctgct cctcggccga ggtgccacgc tcaaggatgg tgtggacgtg
     3121 cgtgatctct ttgcgcgagc cgaggtcgtc caccacgtca tcgacgaact ccagcagctc
     3181 catgatgagg tcgcgcacgg gcacctccgt ctgcttgccg aagtcgatca tcttgccgcc
     3241 tatgccgtag cgcaccgcgc gccacttgtt ctcctgaatg agcatccggc ggtagaggcg
     3301 gaagccgagg ttgcgctcga tgagccggtc gagcttgcag acgatggctt ggaagagggc
     3361 ggcgagggcg agggtgtcgt ctacgcgcgt cgggatgtcg caggcgcgga actccagcgt
     3421 cgggaaggtg gggtgggggc ggatgtccca ccagattttc ttcgggttgc tgatgcagtt
     3481 cgtcttcttc agcagctcga cgtatcgctc gtaagattgg taagagtcga aatggtcggg
     3541 gatgtccgtg cgcggaaact tcttgaagac ctcggagcga tacgacttca gacccgtgtt
     3601 gatgccgagc cagaagggcg aggaggtcga aagcgcgagc atgtgcggga ggaagtagcg
     3661 cgaggcgttc atgatgtgta tctgccgctc gcggtcttcg atgccgacgt ggacgtgaag
     3721 gccgaagatg agcagggagc gcgcgaccat ctgcaactcc tcgatgagca gggcgtagtg
     3781 cgggtcgtcg aacaccttct gatccgtcca ctgcgagatc gggtgagtgg aggcggcgat
     3841 gatcgccaga cccttcttcc gggcgaggtg cgagacgatg gcgcgcaggc gcgtgatgtc
     3901 ggcgcgggcc tcctcaatgt tccgacagat gccggtgccg acctcgacct gcgcctgcat
     3961 catctcgggc ttgacctgct cgccgaggat catcttgccc tcttcgagaa tctccgagac
     4021 gtgcgagacg agcagccgcg tctgcggatc gacgatctga aactcctcct cgatgccgag
     4081 ggtgtacttt tcgaacatcg ttgccctcct tcggaggggt gatgagtgat gagtgatgag
     4141 tgataagaaa gagaaagcgg attttgtcct tcttatcact catcactcat cactcatcac
     4201 ccatttctca gtcccccaac gcccgctcga tggtcgtccg gttctccgcc gtcagcccgt
     4261 ggaatcggag gcccacgccg cggccggggt tgctgtagac cgcctcggcg ttgacgacca
     4321 cgtccccttc ggggaacggg acgcgcaccg tcaggagcgc gccctcgggg atgtcggcct
     4381 gcgtgttcat gtagaggccg ccggggctga tgtccttcga gctggcgacg cccgtcaagt
     4441 ccaccccctc gaagaagacg tcgatgatga gccgcgagcg ctcgaactcg cgccgctcgt
     4501 cctgcgggct gcggctgtcg atgtagtcca tacccttcta tcccttctcg ccgatgatga
     4561 aggtcggcac catgccgccg cccgacgaga cgttggccaa ctccgtggac gacaggcgcg
     4621 tgaaggccga ccccacccgc ccgttgaaaa gcatcgggtc gagatcgacc aactgctcgg
     4681 cgtagacgac cgagccgtcg tcatgcaggg ccgggaaggt ctcgcgggcc gtgcggacgc
     4741 gctcctgcac gatgtagtcg ccgttcgaga gcgcgagggc gagcgcctcg tcccaatccg
     4801 tctcaccgct ccagccgatg tagatgccgt gcccgccgta atcgtcgttg ggcttcaaga
     4861 ccagccggcc gcggttttcg gcgatgaact cgacgaggtc gattccctca cccttgtact
     4921 ccgtgcggcc ctcgcgcacg gttcgcgtcc aggggacgtg gcgcgcgatc acctcgcgct
     4981 catccgcgtt gaagagcggc gcgtggcgct cgtccgtgag gacggcgaag agggccttct
     5041 tgtggatgag cttcgagcgg aagctgttga ccatgcagaa ggcgcgggcg cggtaggcgt
     5101 ccaaaagcgc cgggtgctcc gacatgatcg gcagatactc gttgacgagc aggcgcttgt
     5161 agacgatgtc gatctctcgc ccgccgccgc gcaggcggcc ccccgtgaac tccagctcgt
     5221 cgggcgagca gatgaccgag gggtagccct gcgactcgaa gtactcgcgg aacagctcga
     5281 actccttctg tgtgggcagc ccctttaaat cgacgatggc gatggtcggc ttgtgctcgg
     5341 gcgcgcggcc cgagaattcg cggtgcgctt cgagcagggt ttcgagcatg agcgggcggc
     5401 cgtggaagcg ccgcaggttg tagcgctcac tgaagcggcg catgacgggc agctcggaga
     5461 agatttcgta ggccgcgtcg gcgtaggcga tgccggcggg gctttcgccg ttgagttcga
     5521 cgaaatgata atcgtcttcg atgaggaacg agtcgaggcg ggcagtcggg gagacggcgc
     5581 ggtagccggg gtcgattgag acgaggtcgc gctcgatctc ggtgatgccg agttcgtcga
     5641 ggagcgacgc gtcttcgacg gccgcgtcct tcaccttctg gatagccccc cagaccgtct
     5701 cgcagatgcg gcggacgcgg gcgaagtcgg tctcggtgac gaagtgcggg cgcaggtagg
     5761 gcgacaggcg acggccgccg aagatcagct tggcgcgctc cagaccctcg tcgagcattc
     5821 ggcgcgagcc ttcggccgtc tcttcgtcct ggagcaggcg atgatagtag tcaacggctt
     5881 cttgcaacat gcgcttgaaa cgtggccgaa ctacgggcga attttattca cgagctggtg
     5941 gaggcataga ttaacacaga agggcgtccg ccggtaaaac cccgcggcgt gcggcccggc
     6001 gcggattacg agtcagtgta ctgaatgttt agaggcgggc agtgcccgcc gtctcaacta
     6061 tcggagcttg agacggcggg cactgcccgc ctct
//
LOCUS       DASVES010000183         7623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_781998, whole genome shotgun sequence.
ACCESSION   DASVES010000183 DASVES010000000
VERSION     DASVES010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781998"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1951
                     /locus_tag="VF240_07380"
     CDS             332..1951
                     /locus_tag="VF240_07380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175088.1"
                     /translation="MTYRLAYARLFDEKSAQNYYDVLTRTAENISPLYLLDAFDLAVL
                     GAYFALLALLSVYGAYRVKQVIDFWRYRRVEPRPKSLYAETALPRVTVQLPLFNEMYV
                     VERLLASVAALDYPRERLEIQVLDDSTDETRDVARAAVARLRGHGLDAAYVHRDDRAG
                     FKAGALEAGLRTAKGELVAIFDADFVPRPQTLRKLVHFFNDPLVACAQMRWSHINGRS
                     NLLTRLQTVLLDGHFVVEQTVRNRTGGFFNFNGTAGMWRRRAIELSGGWQHDTLTEDT
                     DLSFRAQLMGWKFVYLLDEDAPSELPVEAGAFKAQQRRWAKGVTEVGLKLYPRILRAE
                     LPRRVKLEMFFRLTGNISYPLMILVSLLQFPLLLVRYNQGLHHLVLLDAPLLVFSTLS
                     VVFFYGTAIWHLDGRDHRRFVYLPLVMALGTGLALSNARAVLEALCGVKTEFVRTPKY
                     KVEGGGDESWKRQKKYGRKLGLLPFVELSFALYFLLAILYAARMGMWATIPFLSLFLF
                     GYGYIGIASLAPGASFRRLVDALRAPAHGRR"
     gene            2009..2533
                     /locus_tag="VF240_07385"
     CDS             2009..2533
                     /locus_tag="VF240_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7175089.1"
                     /translation="MSAPDYLTGGDRVPLAYLITFRCYGTWLHGDERGSTDRHHNRYG
                     TPFIPPDEHWRRYNTRTLRYAPVELDTTQRAAVEASIRETCEARGWALRAVNVRTNHV
                     HAVVSAGCKPERVIVAFKANATHRMRAEECWPHEHSPWSEGGSRRYLWVERNVERAVE
                     YVMYGQGDRPPDFD"
     gene            2715..5699
                     /locus_tag="VF240_07390"
     CDS             2715..5699
                     /locus_tag="VF240_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7175090.1"
                     /translation="MLNRMPPTLARLAAVCALLALVSLSALAQSQATTGNIEGRVLDP
                     QGAVVPGATVTATNKETGLERTATTDDEGNYRVILLPPGVYNVRAAATNFSGAQAADV
                     IVSVGGLTTLDVTLGVSGASEQITVSAEAPVVETTRTSVATTVNQVAIENLPVNGRNY
                     LDFATLTPGVIRDQTRDGDLSVGGQKGTLNNLQVDGTDNNNTFFGQAFGRTGVRPPYQ
                     FSEESVQEFQVNQNGFSAEFGRAGGAVINVVTRSGTNEFHGGLFEYFRDEALNASTPT
                     LKAQQSQRGQPNVRPPLQINQFGGRIGGPIVKNRAFFFFTYDGQRQKLPNPVEPPNLF
                     TAAGATARPLLEPLIGTYQINRDQDVFMLKSDISLNRSNQLTLRFNQQNFTGVNNEFT
                     GPLAVEQHSGDSLAKTTTFSGTLASTFSSSVVNELRFQFARDKEPGTANSDAPEAQIQ
                     TGAGFLLIGRNNFSPRETTIKRAQFVDNLSYVRGAHNFKTGVDFNFDRIFNFFPGFFT
                     GQYTFTSYANFALNRPSSYTQRFALPNTSGATTFPDSTDYAFFLQDDWRVTPRLTVNA
                     GLRWDYQALARPPIQNPDPILLAAGLDTSRQPKDTNNFAPRLGFSYAFDDKSVVRGGY
                     GLFYGRTTAIMLGTAHSGNGVQTTGVTLTCTTTPANPCPTYPNNLTTLPAAGTGRPDL
                     FIFQEGYTQPYVQQARLGYEREVFDNTSFSATIMLYRGVHLSRTRDINLGTPVPTNFT
                     ASDGTLFSVERFPAARPFASYNRISLFESNGNSFYRGLALQLARRLTRGFQYIASYTF
                     SKAEDDKPDSTAVVPGGGDDAKVVQNSLNIRGDYARNDVDLPHRFVFSPIYTFGRYNA
                     DSALVRGLLSDWTVSGILQMQSGYPYSAQIGADVNRDGNSRSDRVPGATRNSFRTPNT
                     YQVDLRLQRAFRFGEDMQLRLIGEAFNVFNRANILTTNTNFFNANTTLLTLTPPTGGA
                     AFGLPRTFQTQRQIQLAIKFEF"
     gene            complement(5796..6536)
                     /locus_tag="VF240_07395"
     CDS             complement(5796..6536)
                     /locus_tag="VF240_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX7175091.1"
                     /translation="MKEFGNRNEQFNQRAWNPEPDVWAAHRARTAAEATTVERMSFVR
                     KVYALFFAATLFAVGGVALGFVYPPLMMTVAAHPWLSLFALLGGVMGAQAVRLIPGVN
                     LVALFAFTTLTGAIISPAIYLYSQINPASLVQAGVLTVGIFGGLTAYVFITKRDFSFM
                     RGMLMTGLIVMVLAVVLNIFVASSAFSFAIAAASLLLFSGYVLYDTSNIIRRYPTNEY
                     VAGALSLYLDAFNIFLALLRLLNGGNRN"
     gene            6815..>7623
                     /locus_tag="VF240_07400"
     CDS             6815..>7623
                     /locus_tag="VF240_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7175092.1"
                     /translation="MIAQTHSSGAGASSDAGQTPPILFVLAAALLWSTGGLFIKATEL
                     SAFELSFGRSLLAALTVAYFTRRRGFSLNTLTTIAAVLYAALLLLFVLATKQTTAANA
                     IFLQYTAPVYILVLEPLIFKEKYRRRDIAVVAACVAGIALFFVGRVRPEEMQGNLLAL
                     VSGVCFAAFILIFRHPRAGRVNRASSIIYGNLLLAAVCAPSFFSALDRVTLSDLGIIT
                     FLGVFQIGVAYTLFTRGIERGVRSLDAGLVGYVEPVSNPVWVYLFIGERPSP"
BASE COUNT         1329 a         2805 c         2195 g         1294 t
ORIGIN      
        1 gagacgcgga cgcgacgacg actgcccatc cggggaatgg aactgccgcc ccgcataatt
       61 ctcccgcgcc ggcgcttgcg ccggtgacaa tccccgcatc taagcgggcg gccgccctcc
      121 cacgcggggg gcggccgccc gcttcgcgcg ttcggggcgg caatgaacag taccgcgagc
      181 ggtagcgagc gggtatgcct gcgaccagag tcgcgcatcc cataaccaca cccgcgaggc
      241 ttccgctcgc ggtactgatg tcgatgagtc gagtcgcggc gcaggcttca gacatgcccc
      301 cgcgcggctt cttagtcgga ggtggacact gatgacttac cggctcgcct acgcccggct
      361 cttcgacgag aagtcggcgc agaattatta cgacgtgctc acccgcacgg ccgagaatat
      421 cagcccgctc tatctcctcg acgccttcga cctcgcagtc ctcggcgcat acttcgcgct
      481 cctcgcgctc ctctccgtct acggcgctta ccgcgtcaaa caggtcatcg acttctggcg
      541 ctaccgacgc gtcgagccgc gccccaaatc tctctacgcc gaaacggcgc tgccgcgcgt
      601 caccgtccag ctcccgctct tcaacgagat gtacgtcgtc gagcgcctgc ttgcgtcggt
      661 cgcggcgctc gactacccgc gcgaacgtct ggagattcaa gtcctcgacg actcgaccga
      721 cgagacgcgc gacgtggccc gcgccgccgt cgcgcgcctg cgcggccacg gactcgacgc
      781 cgcctacgtt caccgcgacg accgcgcggg gtttaaggcg ggcgcgctcg aagccgggct
      841 gcggacggcg aagggcgaac tcgtcgccat cttcgacgcc gacttcgtcc cccggcctca
      901 gacgcttcgc aagctcgtcc acttcttcaa cgacccgctc gtcgcctgcg cgcagatgcg
      961 ctggtcgcac atcaacggcc gctcgaatct gctcacgcgc ctccagaccg ttctgctcga
     1021 cggccacttc gtcgtcgagc agacggttcg caatcgcacg ggcggcttct tcaacttcaa
     1081 cgggacggcc ggcatgtggc gtcgccgcgc catcgagcta tccggcggct ggcagcacga
     1141 cacgctcacg gaggacaccg atctgtcctt ccgcgcgcag ttgatgggct ggaagttcgt
     1201 ctacctgctc gacgaagacg cgccatcgga gttgcccgtc gaagcggggg ccttcaaggc
     1261 gcagcagcgc cgctgggcga agggcgtcac cgaggtcgga ctcaaactct acccgcgcat
     1321 cctgcgcgcg gagctgcccc ggcgcgtgaa gctggagatg ttcttccgcc tcaccggcaa
     1381 catcagctac ccgctgatga ttctggtgag cctcctgcag ttcccgctcc tgctcgtgcg
     1441 ctacaatcag ggcttacacc acctcgtcct actcgacgcg ccgctcctcg tcttctccac
     1501 cctctcggtc gttttcttct acggcacggc catctggcat ctggacgggc gcgaccaccg
     1561 ccgcttcgtc tacctgccgc tggtcatggc gctcggcacg ggccttgcgc tctcgaacgc
     1621 gcgcgccgtc ctcgaagccc tctgcggggt gaagaccgag ttcgtccgca cgcccaagta
     1681 caaggtcgag ggcggcggcg acgagtcgtg gaagcgacag aagaaatacg gacgcaaact
     1741 cggcctcctg cccttcgtcg aactctcctt cgccctctac ttcctgctcg ccatcctcta
     1801 cgccgcgcgc atggggatgt gggccacgat ccctttcctc tccctcttcc tcttcggcta
     1861 cggctacatc ggaatcgcga gcctcgctcc gggcgcgagc ttcagacgtc tcgttgacgc
     1921 gttgcgcgcc cccgcgcacg gccgccggtg agtcgcccaa gcgacacaac agtaccgcga
     1981 gcggtagcga gcgggtatac tccgcggcat gagcgcgcct gactacctca caggcggcga
     2041 ccgcgtcccg ctcgcgtacc tcatcacatt ccgctgttac ggcacctggc tgcacggcga
     2101 tgagcgcggc tcgaccgacc gccaccacaa ccgctacggc acgccgttca tcccgcccga
     2161 cgaacattgg aggcgataca acacgcgcac gctcagatac gcgccggtcg agctagacac
     2221 tacgcagcgg gccgcggtcg aggcgtccat ccgggagacg tgtgaggccc gcggttgggc
     2281 gttgcgtgcg gttaacgtca gaaccaatca cgtccacgcc gtcgtctcgg caggttgcaa
     2341 gccggaacgg gtgatagtcg ccttcaaggc taacgcgaca cacagaatga gagcagagga
     2401 atgctggccg cacgagcaca gcccgtggtc ggaaggaggt agccggcgtt acctgtgggt
     2461 ggagcgtaac gttgagcggg cagttgaata tgtgatgtat gggcaaggtg atcgaccgcc
     2521 ggattttgat tgagagtaga gcttttgacc aagccacccg ctcgctaccg ctcgcggtac
     2581 tgtgattgca gccacttcaa ccttgaccct tgggcgcgcg cgccttagta tctccgccga
     2641 gcttttcgcc ttgttgctgg ggcgcgctcc ccggccttcc attctgacca catcgcttca
     2701 tcgaagagga atctatgctc aacagaatgc cccccacgct cgcgcgcctc gccgccgtgt
     2761 gcgcgctgct cgcgctcgta tctctgtcgg ccctcgcaca gtcgcaggcg accaccggca
     2821 acatcgaggg ccgcgtgctc gacccgcagg gcgcggtcgt ccccggcgcc accgtcaccg
     2881 ccacgaacaa ggagaccggc cttgagcgga cggcgacgac ggacgacgaa ggcaactacc
     2941 gcgtcatcct gctgccgccc ggcgtctaca acgtgcgcgc cgcggcgacg aacttctccg
     3001 gcgcgcaggc cgcggacgtg atcgtctcgg tcggcggcct gaccacactc gacgtgacgc
     3061 tcggcgtcag cggcgcctcc gagcagatta cggtctcggc cgaggcgccc gtcgtcgaga
     3121 ccacgcgcac gtccgtggcg actacggtca atcaggtcgc catcgagaac ctgcccgtca
     3181 acggccgcaa ctacctcgac ttcgccacgc tcacgcccgg cgtcatccgc gaccagacca
     3241 gagacggcga cctctccgtc ggcgggcaga agggaactct caacaatctt caggtggatg
     3301 gcaccgacaa caacaacacc ttcttcggac aagccttcgg ccgcacgggc gttcggcccc
     3361 cgtatcaatt ctccgaggag tcggtgcagg agtttcaggt caaccagaac ggcttctcgg
     3421 ccgagttcgg ccgcgcgggc ggcgccgtca tcaacgtcgt cacgcgcagt gggacgaacg
     3481 agtttcacgg cggactcttc gagtacttcc gcgacgaggc tctgaacgcc agcacgccga
     3541 cgctcaaggc gcagcagtcg caacgcggcc agcccaacgt ccggcccccg ctccagatca
     3601 accagttcgg cgggcgcatc ggcggcccca tcgtcaagaa ccgcgccttc ttcttcttca
     3661 cctacgacgg ccagcggcag aagctgccga acccggtgga gccgccgaac ctcttcaccg
     3721 ccgccggcgc aaccgcgcgc ccgctgctgg aacctctgat cggcacctat cagatcaacc
     3781 gcgaccagga tgtcttcatg ctcaagtccg acatctccct gaaccgcagc aaccagttga
     3841 cgctccgctt caaccagcag aacttcaccg gcgtcaacaa cgagttcacc ggcccgctcg
     3901 ccgtcgagca gcactccggc gacagtctcg ccaagacgac gactttctca ggcacgctcg
     3961 cctcgacgtt tagcagctcc gtcgtcaacg agctgcgctt tcagttcgcg cgcgacaagg
     4021 agccgggcac ggccaactcc gacgcccccg aggcgcagat tcaaaccggc gccggcttcc
     4081 tcctcatcgg ccgcaacaac ttcagccccc gcgaaaccac catcaagcgc gcacagttcg
     4141 ttgataacct ctcctacgtg cgcggcgcac ataacttcaa gaccggcgtc gatttcaact
     4201 tcgaccgcat cttcaacttc ttccccggct tctttaccgg gcagtacacc tttacctcct
     4261 acgcgaactt cgccctcaac cgcccgtcga gctacacgca acgcttcgcc cttcccaaca
     4321 cctcgggcgc gacgaccttc cccgacagca ccgactacgc tttcttccta caggacgact
     4381 ggcgcgtcac cccgcgcctg accgtcaacg ccggcctgcg ctgggactat caggcgctcg
     4441 cccgtccgcc catccagaac cccgacccca tcctcctcgc cgccggcctc gacaccagcc
     4501 gccagccgaa ggacaccaac aacttcgccc cgcgcctcgg cttctcttac gccttcgacg
     4561 acaagtcggt cgtgcgcggc ggctacggcc tcttctacgg ccgcaccacg gccatcatgc
     4621 tgggcacggc ccattcgggc aacggcgtgc agaccaccgg cgtcacgctc acctgcacga
     4681 cgacgccggc caacccgtgc cccacctacc cgaacaacct gacaactctg cccgcggccg
     4741 ggacgggccg ccccgacctc ttcatctttc aggagggcta cacgcagccc tacgtccagc
     4801 aggcgcgtct cggctacgag cgcgaggtct tcgataacac cagcttctcg gcgaccatca
     4861 tgctctaccg cggcgtccac ctctcgcgca cgcgcgacat caacctcggc acgcccgtcc
     4921 ccaccaactt cacggcctcc gacggcacac tcttctccgt cgaacgcttc cccgcggcgc
     4981 ggcccttcgc cagctacaac cgcatcagtc tcttcgagag caacggcaac tcgttctatc
     5041 gcggcctcgc cctgcaactg gcgcggcgtc tgacgcgcgg cttccagtac atcgcctcct
     5101 acaccttctc gaaggccgag gacgacaagc ccgactcgac ggccgtcgtc cccggcggcg
     5161 gcgacgacgc caaggtcgtc cagaactcgc tcaacatccg cggcgactac gcgcgcaacg
     5221 acgttgacct cccgcaccgc ttcgtcttca gccccatcta caccttcggc cgctacaacg
     5281 ccgacagcgc gctcgtgcgc ggcctgctga gcgactggac ggtctccggc atcctccaga
     5341 tgcaatccgg ctacccctac tcggcgcaga tcggcgcgga cgtgaaccgc gacggcaact
     5401 cgcgcagcga ccgcgtcccc ggcgccaccc gcaacagctt ccgcactccc aacacctatc
     5461 aggtggactt gcgcctccag cgcgccttcc gcttcggcga ggacatgcaa ctccggctca
     5521 tcggcgaggc tttcaacgtc ttcaaccgcg ccaacatcct caccacgaac acgaacttct
     5581 ttaacgccaa caccaccctg ctcacgctga cgccccccac cggcggcgcc gccttcggcc
     5641 tgccccgcac cttccagacg cagcggcaga ttcagttggc catcaagttc gagttctgaa
     5701 gacagaagac aatcgccgtt caaacaaaaa aggcggggcg ttcacgaatg aacgccccgc
     5761 cttttagttt tcgcgcgcgc gtgcgccggg ttcggttagt tcctgttgcc gccgttgagt
     5821 agtcggagca gcgcgaggaa gatgttgaag gcgtcgaggt agagcgagag cgcgccggcc
     5881 acgtactcgt tcgtcgggta gcgccggatg atgttcgacg tgtcgtagag cacgtagccc
     5941 gagaagagca gcagcgacgc cgccgcgatg gcgaacgaaa acgcgctcga ggcgacgaag
     6001 atgttgagga cgacggccag caccatgacg atcagccccg tcatcaacat cccgcgcatg
     6061 aagctgaagt cgcgcttggt gatgaagacg taggccgtca aacccccgaa gatgccgacc
     6121 gtcagcacgc ccgcctggac gagcgaggcc gggttgatct ggctgtagag atagatggcc
     6181 ggcgagatga tggcgcccgt gagcgtcgtg aaggcgaaga gcgcgacgag gttgacgccg
     6241 gggatgaggc gcacggcctg cgctcccatc acgcccccga gcagcgcgaa cagggagagc
     6301 cacgggtgag cagccaccgt catcatcagc ggcgggtaga cgaacccgag cgccacgccg
     6361 ccgacggcga acagcgtcgc ggcgaagaag agcgcgtaga ccttgcggac gaacgacatg
     6421 cgctcgaccg tcgtcgcctc ggcggcggtg cgcgcccggt gcgcggccca cacgtccggc
     6481 tccgggttcc acgcccgctg gttgaattgt tcgtttcggt tgccgaattc tttcatcgaa
     6541 atcctctctt gagctgaatc gccgcacctc gcggcggtcg tttcattaga tgcgtcggcg
     6601 cggccggccg ggagaccggc aaaacaaaaa attacttcaa agagaacacc tattcaattg
     6661 tcgcctcacc agccgctcga ctgaaattat acacgatagc ggcggccgtg tgcctcacca
     6721 catcgggcgc gagcgtctta ccccgacgct ggaattcgcc agcgttctat ggtttccccg
     6781 agggatgctt tatcatcccc gctcgacctg accgatgata gcgcagacac acagcagtgg
     6841 cgcgggcgca agttccgacg cgggccaaac cccgccgata ctcttcgtgc tcgccgcggc
     6901 gctcctgtgg agcacgggcg gtctgttcat caaggcgacg gagctttcgg ccttcgagct
     6961 gtcgttcggc cgctcgctgc tggcggccct gacggtcgcc tacttcacgc gccgccgggg
     7021 gtttagcctc aacaccttga cgacaatcgc cgccgtgctc tacgcggcgc tcctcctgct
     7081 tttcgtgctg gcgaccaagc agacgacggc cgccaacgcc atcttccttc agtacaccgc
     7141 gcccgtctac atcctcgtcc tcgagccgct catcttcaaa gaaaaatacc ggcggcgcga
     7201 catcgccgtc gtggcggcct gcgtggccgg catcgctctc ttcttcgtcg gacgggtgcg
     7261 gcccgaggag atgcaaggca acctgctggc gcttgtgtcg ggcgtctgct tcgccgcctt
     7321 catcctcatc ttcagacacc cgcgcgccgg ccgggtcaat cgcgcctcgt cgatcatcta
     7381 cggcaacctg ttgctggcgg ccgtttgcgc gccgtcgttc ttctccgccc tcgacagggt
     7441 gacgctctcc gacctcggca tcatcacctt cctcggcgtc ttccagatcg gcgtggccta
     7501 caccttgttc acgcgcggca tcgagcgcgg cgtgcgctcg ctcgacgcgg gtctggtcgg
     7561 ctacgtcgag ccggtgtcga accccgtgtg ggtctacctc ttcatcggcg agcgcccctc
     7621 gcc
//
LOCUS       DASVES010000184         5730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_92682, whole genome shotgun sequence.
ACCESSION   DASVES010000184 DASVES010000000
VERSION     DASVES010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92682"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..730
                     /locus_tag="VF240_07405"
     CDS             221..730
                     /locus_tag="VF240_07405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7175093.1"
                     /translation="MPVLHDRPVGMQDEKQSVRFNIRPAAPGEAASLTELAVRSKSHW
                     GYDAEFIDDSRADLTVTPNAIDAGRVFVAESAGVVAGFYSLSGEGEEAEVELLFVEPR
                     QIGTGAGRALWRHAAATARRLGFRTLVVASDPFAEPFYRAMGAERVGERESIVRAGRM
                     LPLLRYPLD"
     gene            929..2557
                     /locus_tag="VF240_07410"
     CDS             929..2557
                     /locus_tag="VF240_07410"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX7175094.1"
                     /translation="MTTSTETRTRLRQLTGELRALEARLRLGGGEDKIERQHQQGKLT
                     ARERTARLFDRDSYTQEIGLLVAYDEYKGAAPAAGVVTSVGRVSGRECVVVANDATVK
                     AGSWWPETIKKILRAQEIAMRSRVPIIYLVDSAGVNLPYQGGVFPGQYGAARIFYYNS
                     IMRRYLHVPQVSAVMGPCVAGGAYLPALSDIIIMVEGTSFMGLGGANLVKGATGQTID
                     NETLGGARTHTEVSGVAHYRAKTDEECIEKIREVVSELPAAPATRVSTSEPSDAARPL
                     EDLYEIIPEDHRHPYDAREVLRCLLDGGHLDEFQSDYASEMVTGHARLRGLQVGVIAN
                     NRGLIRRPGGGAPKFGGIIYTESAEKVAYFIETCNRHRTPLVFVQDVSGFMVGAEAEH
                     SGIIRAGARFVEAMATASVPKIVLTINHASGAGYYAMAGQGFDPDFIFSWPTGRMGVM
                     EGDSAVMAIYGTQLEKLKREGREPDERMREEMERVRADYDQQLDARYAAARGFVDAVL
                     APEETRDALELALRVSLNFDGPHLGAFVLPPTLA"
     gene            2647..3297
                     /locus_tag="VF240_07415"
     CDS             2647..3297
                     /locus_tag="VF240_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175095.1"
                     /translation="MNRKSIVKSVFGLFAAVCAALAFVVADVGALTQNTNSSTTEQNT
                     NSDTTMMEGNANTGRRARRGRRGRRARPAAAANADASGAMTDNTNTSDAMPAQDAGMA
                     DTQSTGGGTNEDVSGTYAGRVRMSGGHEMNGEGTLTITGNTFTIESEGMTHNGRIYAV
                     NTRKYIGAALIFEDLTDSTTNTPLACSVRVRMGRTFSATPVPGSRNKCWFAGRKTG"
     gene            3544..4911
                     /locus_tag="VF240_07420"
     CDS             3544..4911
                     /locus_tag="VF240_07420"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX7175096.1"
                     /translation="MREKIRIACGQGFWGDMLDAPVRQVEDGPIDYLMLDYLAEVTMS
                     IMQKQRARDPEAGYARDFVPLMRDILPACVERDIRVTANAGGVNPQGCAAAVREAARS
                     VGLGGKLKIGIVSGDDIMGRLDEFLARGVELRNMETGEPLSAVRASIQSANVYLGAWP
                     MVEALARDARVVITGRATDTGLTLAPLIHEFGWGETDWDRLAAGTVAGHIIECGAQCS
                     GGNCQYEWESIPDMANVGYPIVEAQADGSFIVTKHDGTGGRVSVPSVKEQLVYEMGDP
                     GEYITPDCVADFTSIRLEEAGTDRVRVHSVRGKPATEFYKVSISYSAGYKAVGTLVYS
                     WPDAHRKARAADQILRARLERLGLKFDEILTEFVGASATHGPLAGEPSPDAPEVQLRV
                     GVRGPERAAVERFTKELAPLILTGPPAVTGFAGGRPKVEEVVAYWPALIPKTEITPRV
                     EVEEV"
     gene            4908..5519
                     /locus_tag="VF240_07425"
     CDS             4908..5519
                     /locus_tag="VF240_07425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7175097.1"
                     /translation="MSEGRNSRRVDEEDAGEASLAWTRESSEPLADCRVFSVRRDMSR
                     SPRDGGAHDFYVIEAPDWINVIPLTADERVVMIEQYRHGIEGVTLEIPGGMVDPGETP
                     RETAAREMLEETGYAARELVALGSLHPNPAIQNNRIHTFLARDAEFQGAPVFDGTEQT
                     VVRLVPLARVPALIADGTITHSLVVAGFYWLWLSQNGFVEGAA"
BASE COUNT         1010 a         1888 c         1940 g          892 t
ORIGIN      
        1 ctttctcgcc tccgagcgcg cctcctggat caccggcgcc acccactcca tcgacggcgg
       61 acgcgcgcag acctgcgccc gctaagaaca gtatgtacac acgtgggaca ggcattcctg
      121 cctgtcatga gcgcagagcg cgaacaggag attcgccggg tctcaacgtc gaataggcgc
      181 gaacagttta ttcgcgctga agcgctcaag acaggcagga atgcctgtcc tacatgaccg
      241 ccccgtaggc atgcaggacg aaaaacaatc cgtgcgtttc aacatccgac cggccgcgcc
      301 gggcgaggcc gcgtcgctca cggaactcgc cgtccgctcc aagtctcact ggggctacga
      361 cgctgagttt attgatgaca gccgtgctga tttgaccgtc actcccaacg caatcgacgc
      421 cggacgcgtc ttcgtcgccg aatcggcggg cgtcgtcgcc ggcttctaca gcctgagcgg
      481 cgagggcgaa gaggcggagg tggaattgct cttcgtcgag ccgcgtcaga tcggcacggg
      541 agcgggccgc gccctctggc gtcacgccgc cgcgacggcc cgccggctcg gctttcgcac
      601 cctcgtcgtc gcgagcgacc ccttcgccga acccttctac cgcgcgatgg gcgccgagcg
      661 cgtcggcgag cgggagtcga ttgtccgcgc cgggcgaatg ctgccgttgc ttcgatatcc
      721 gctcgactaa acccgtggcg cggcctcgac tgaacggccc gcggtcgagc gcgttgtccg
      781 accccgcaca gaagcgcatt gtcttgcctc gcgctcatgt gctatttttc agccatcttc
      841 aagctctgaa atcatgtacg aggcgggcgg cgggccactt tttccgaagg ccggtctccg
      901 tcgtgatgct gtgacgaagt ccgaagcgat gacgacatcc acagagacac gcacacgtct
      961 gcgccagttg accggcgaac tccgcgcgct cgaagcgcgc ctgcgtctgg gcggcggtga
     1021 agacaagatc gagcgccagc accagcaggg caagctcacc gcgcgcgagc gcaccgcgcg
     1081 gctcttcgac cgcgactcct acacgcagga gatcggcctg ctcgtcgcct acgacgagta
     1141 caagggcgcg gccccggccg ccggcgtcgt cacatcggtc gggcgcgtga gcgggcgcga
     1201 gtgcgtggtc gtcgccaacg acgcgaccgt caaggccggc tcgtggtggc ccgagacgat
     1261 caagaaaatc ctgcgggcgc aggagatcgc gatgcgctcg cgggtgccga tcatctacct
     1321 cgtcgattcg gcgggcgtga acctgcccta tcagggcgga gtctttcccg gtcagtacgg
     1381 cgcggcgcgc atcttttatt acaactcgat catgcgccgc tatctgcacg tcccgcaggt
     1441 gtcggccgtg atggggccgt gcgtggcggg cggcgcatac ctgcccgcgc tctccgacat
     1501 catcatcatg gtcgagggca cttcgttcat gggcctcggc ggcgcgaacc tcgtcaaggg
     1561 cgcgacgggg cagaccatcg acaacgagac gctgggcggg gcgcgcacgc acacggaagt
     1621 ttccggcgtc gcccactacc gcgcgaaaac ggacgaggag tgcatcgaga agattcgcga
     1681 ggtcgtctcg gagttgccgg ccgcgccggc gacacgcgtc tcgacgagcg agccgagcga
     1741 tgcggcccgg cccttggaag acctctacga gatcatcccc gaagaccacc ggcatcctta
     1801 cgacgcgcgc gaagtcctgc gctgcctgct cgacggcggg catctcgatg agtttcagtc
     1861 cgactacgcc tccgagatgg tcacgggcca tgcgcgcctg cgagggcttc aggtcggcgt
     1921 catcgccaac aaccgcggcc tcatccgtcg ccccggaggc ggagcgccga aattcggcgg
     1981 catcatctac acggagtcgg ccgagaaggt cgcttacttc atcgagacgt gcaaccgaca
     2041 tcgcacgccg ctcgtgttcg tgcaggacgt gtcgggcttc atggtcgggg ccgaggccga
     2101 gcacagtggg atcatccgcg ccggggcgcg cttcgtcgag gcgatggcga cggcctccgt
     2161 gccgaagatc gtgctcacga tcaaccacgc gtcgggcgcc ggctactacg cgatggccgg
     2221 gcagggcttc gaccccgact tcatcttcag ttggcccacc ggaaggatgg gcgtgatgga
     2281 gggcgactcg gcggtgatgg ccatctacgg cacgcagctc gaaaagctca aacgcgaagg
     2341 gcgtgagccg gacgaacgaa tgcgcgagga gatggagcgc gtccgcgccg actacgacca
     2401 gcaactcgac gcccggtacg ccgccgcgcg cggcttcgtg gacgccgtgc tggcccccga
     2461 ggagacgcgc gacgcgctgg agctggccct gcgcgtgagc ctcaacttcg acgggccgca
     2521 cctcggcgcc ttcgtcctgc ccccaacgct tgcgtgagcg caccgtaagg ggcggcaaag
     2581 tttcaggggg gaatagtttg gaataaagtc gttccaacgc agctttggac aggaggagaa
     2641 accaccatga acaggaagag catcgtcaag tcggtcttcg gtctcttcgc ggccgtgtgc
     2701 gccgcactcg ccttcgtcgt cgccgatgtc ggcgcactca cgcagaacac caacagcagt
     2761 acgacggagc agaacaccaa cagcgacacg accatgatgg agggcaacgc gaacacgggc
     2821 cggcgcgctc ggcgtggccg gcgcggccgc cgcgcgcgcc ccgcggccgc cgccaacgcg
     2881 gacgcgtccg gcgcgatgac cgataacacg aacacgtccg acgccatgcc ggcgcaggac
     2941 gccggcatgg ccgacacgca gagcaccggc ggcgggacga acgaagacgt gtcgggcacc
     3001 tacgcgggcc gcgtcagaat gtccggcggc cacgagatga acggggaggg gactctcacc
     3061 atcaccggca acaccttcac catcgagtcc gaggggatga cgcacaacgg gcgcatctac
     3121 gccgtgaaca cgcgcaagta catcggcgcg gcgctcatct tcgaggactt gaccgactcg
     3181 acgaccaaca cgccgctggc gtgctcggtg cgcgtccgca tgggccgcac attctcagcg
     3241 acgcccgtgc ccggctcgcg caataagtgc tggttcgcgg gccgcaagac cgggtagggg
     3301 aggcggacgt tgcggcactt cgccggcgga cgcgggcgtc gctgatcaca gcgtcgccgg
     3361 aggggatggc ccgccgtcgg ggtgtcagat gcaccgccac ggccgaaggc ttcgggcgga
     3421 cggcgcaccc tcgttcgggg gtgtgtcgcc cgcccgcttt gcttgggcgc gccgcgggtg
     3481 gtctaaaccg agtttcttta ttaaacttcc ccatatccta atcgccaacg agtcgggagt
     3541 tgaatgagag agaagattcg catcgcctgc gggcagggct tctggggcga catgctcgac
     3601 gcgcccgtgc gtcaggtcga ggacggcccc atcgattacc tgatgctcga ctatctggcc
     3661 gaggtcacga tgtcgatcat gcagaagcag cgagcgcgcg accccgaggc cggctacgcg
     3721 cgcgacttcg tgccgcttat gcgcgacatc ctgccggcgt gcgtcgagcg cgacatccgc
     3781 gtgacggcca acgcgggagg cgtcaatccg caggggtgcg ccgcggcagt gcgtgaggcg
     3841 gcgcgtagcg tcggcctcgg cgggaagctc aagatcggca tcgtcagcgg cgacgacatc
     3901 atgggccgtc tcgacgaatt cctcgcccgc ggggtcgagc tgcgtaacat ggagacgggc
     3961 gagccgctct cggcagtgcg cgcctcgatc cagagcgcca acgtctatct cggcgcgtgg
     4021 ccgatggtcg aagcccttgc gcgcgacgcg cgcgtcgtca tcacgggacg cgccacggac
     4081 acagggttaa cgctcgcgcc gctcattcac gaattcggct ggggcgagac ggactgggat
     4141 cggctcgccg ccggcaccgt cgccggtcac atcatcgagt gcggcgcaca gtgttcgggc
     4201 ggcaactgcc agtatgagtg ggagtccatc cccgacatgg cgaacgtcgg ctaccccatc
     4261 gttgaggcgc aagctgacgg ctctttcatc gtcaccaagc acgatgggac gggcgggcgc
     4321 gtctcggtgc cttcggtaaa ggaacaactc gtttacgaaa tgggcgaccc cggcgaatac
     4381 atcacgcccg attgcgtcgc cgacttcacc tccatccgtc tcgaagaggc ggggacagac
     4441 cgcgtgcgtg tccactcggt caggggcaaa cccgcgacgg agttttacaa ggtctcaatc
     4501 agctactcgg ccggctacaa ggccgtcggc acgctcgtct attcgtggcc cgatgcacac
     4561 aggaaggcgc gcgccgccga ccaaatcctg cgcgcgcgcc tcgaacggct cggcctcaag
     4621 ttcgacgaaa tactcacgga gttcgtcggc gcgagcgcga cgcacgggcc gctggccggc
     4681 gagccttcgc cggacgcgcc cgaagtgcaa ctgcgtgtcg gcgtgcgtgg gccggagcgc
     4741 gcggccgtcg agcgtttcac gaaggagttg gcgccgctca tcctgacggg gccgccggcc
     4801 gtcacgggct ttgcgggcgg tcgcccgaag gtggaggagg tcgtggccta ctggccggcg
     4861 ctcatcccga agacggagat cacgccgcgc gtggaggtcg aggaagtttg agcgaaggcc
     4921 gcaacagcag gcgcgtggat gaagaggacg ctggcgaggc gtcgctcgca tggacgcgcg
     4981 agagttctga gccgctggcc gactgtcgcg tcttcagcgt ccggcgcgac atgagtcgca
     5041 gcccgcgcga cgggggcgcg cacgacttct acgtcatcga agcgcccgac tggatcaacg
     5101 tcatcccgct gacggcggac gagcgcgtgg tgatgatcga gcagtacagg cacggcattg
     5161 aaggtgtgac gctggagata ccgggcggca tggttgaccc cggcgagacg ccgcgcgaga
     5221 cggccgcgcg cgaaatgctc gaagagaccg gctacgccgc gcgcgagctg gtcgcgctcg
     5281 ggagcctgca ccccaacccg gcgatccaga acaaccggat tcacaccttc ctcgcgcgtg
     5341 acgccgagtt ccaaggcgcg cccgtcttcg acggcacaga gcagaccgtc gtccggctcg
     5401 tcccgctcgc gcgcgtcccc gcgctcatcg ccgacgggac gatcacacac tcgctcgtcg
     5461 tggcggggtt ctactggctg tggctatcgc aaaacggctt cgtcgaaggg gcggcatgat
     5521 cgtgagtgag ggatactcag ttctcatcat tgacatgacc tacgaccgcg aaaaggattt
     5581 tgtgtttagc ggctgtccga ccgtcgagct ggcgaacgaa ttcgcccggc gctgggttcg
     5641 cgactcggtc gaagagttgc gggccgctaa ccagacgagg gaggagttgc gccgattgtg
     5701 gttcattttc ggtgaagacg cgaccgtgct
//
LOCUS       DASVES010000185         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1404150, whole genome shotgun sequence.
ACCESSION   DASVES010000185 DASVES010000000
VERSION     DASVES010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404150"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /locus_tag="VF240_07430"
     CDS             complement(<1..2011)
                     /locus_tag="VF240_07430"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX7175098.1"
                     /translation="MSDQRERLAELTSDDPELKEAPLRRDVRSLGHLLGRMLKEQAGD
                     ELFEKVESLRRLTSEQRESSASLGPATARRATGESDLMAKVGELIGRLTAAEVYQITK
                     AFAVYFELINLAETNHRKRRRRARTLKAELPALPGTFRGTLARLRDSGLGWEAVLEML
                     RQVEVTPVFTAHPTEVARRTVLFKRRRIAAQLERLDRLPLSDEEALVADEGIAREVTA
                     LWQTDETRRRQPTVTDEIKMGLDYYPAVLIDTLPDVYAEMAGALNETYELNLTPRQLP
                     SVVSMGSWIGGDRDGNPHVTPETTRAALLMARHTILDHYIKATDRLQERISLSVHQVK
                     VSARLRSNLEAYRVSLTPALAEVQEHPEAEIYRRFLAFVLYRLRASRDLPAAGDYPYA
                     DAASYVTDLRVVRESLAENNGAALAEGLLDPLLRQAETFGFHLHTLDIRQHARVHRKA
                     LRELARGRQFDPDAHGELSTDDDNACDSPTTSAETAALLDSLRAVAELKRSFPPQAIR
                     AYVISGAQTEQDVFALVWLMGVCGVNVAANRELGDPGLMPVPLFESIEDLRRAPEVCQ
                     TLWTSPRYSRLLDSWNREQEIMLGYSDSNKDGGMLTSTWEIYKAHQALHRVAGECGVR
                     LRLFHGRGGTVGRGGGPTQRAINAQPPGAFMGKLRITEQGEVLNFK"
BASE COUNT          355 a          679 c          638 g          418 t
ORIGIN      
        1 acttgaaatt caagacctct ccctgctccg tgattctcaa cttgcccatg aaggctccgg
       61 gcggttgtgc gttgatcgcg cgctgcgtcg gcccgccccc tctgccaacg gttcctcccc
      121 gcccgtgaaa gagtcgcagc cgcacgccgc actcgcccgc aacgcggtgg agcgcctgat
      181 gcgccttgta aatttcccac gtactggtga gcatcccgcc gtccttgttc gagtccgagt
      241 agccgagcat gatctcctgc tcccggttcc acgagtcgag cagacgggag tagcgcggcg
      301 aagtccacaa cgtttggcag acttcgggcg ctcggcgcaa gtcctcgatt gactcgaaga
      361 gcggcacggg catcaaccct ggatccccca gttctctatt ggccgcgacg ttcactccgc
      421 aaacccccat cagccacacg agcgcgaaga cgtcttgttc cgtctgcgcg ccgctgatga
      481 cgtaggcccg gatcgcctgc ggcggaaagc tgcgcttcag ttccgccacc gcgcgcaggc
      541 tgtcgagaag cgcggcggtc tcggcagaag tcgtaggtga gtcgcacgcg ttgtcgtcgt
      601 cggtgctcag ttcgccgtga gcgtcgggat caaactgccg cccgcgtgcg agttcacgca
      661 aggcctttct gtggactcgc gcgtgctgcc tgatgtcaag cgtgtgcagg tgaaagccga
      721 acgtctcggc ctgccgcagg agggggtcga gcagcccttc ggccaacgcc gcgccgttgt
      781 tctccgcgag gctttcacgg acgacgcgca aatcggtgac gtacgaggct gcgtccgcat
      841 aaggatagtc gcccgcggcg ggcaagtcgc gcgacgcccg cagccggtaa agcacgaacg
      901 ccagaaagcg ccggtagatt tcggcctccg ggtgctcttg gacttcggca agcgccgggg
      961 tcaaactcac acggtacgct tcgaggtttg agcgcagccg cgcggaaact ttcacttgat
     1021 gaaccgatag gctgattcgc tcctgcaagc ggtctgtggc cttaatgtag tggtcgagaa
     1081 tcgtgtggcg cgccatcaag agcgccgcac gcgtcgtctc cggggtgaca tgaggattgc
     1141 cgtcgcggtc gccgccgatc caagacccca tgctgaccac ggagggtagc tgtcgcggcg
     1201 tcagattgag ttcgtatgtt tcattgagcg cgccggccat ctccgcataa acgtccggca
     1261 gcgtgtctat caaaaccgcg gggtagtagt cgagacccat cttaatctcg tccgtcacgg
     1321 tgggctgacg gcgacgcgtc tcatccgtct gccacagcgc cgtaacctct cgcgcgatcc
     1381 cttcatcggc gaccagcgcc tcttcgtccg agagcggcag acggtcaagg cgttcgagtt
     1441 gtgccgcgat tctccgacgc ttgaagagca ccgtgcgtcg cgcgacttcg gtcgggtgcg
     1501 ccgtgaagac gggtgtgact tcgacctgtc gcaacatttc gagaaccgcc tcccaaccga
     1561 gaccactgtc gcgcaggcgc gcgagtgttc cgcggaatgt tcccggcagg gcgggcagtt
     1621 cggctttgag tgtgcgagcg cggcggcggc gcttgcggtg gttggtctcg gcgagattta
     1681 tgagttcaaa gtagacggca aaggctttcg tgatttgata aacctcggca gccgtcaggc
     1741 ggccaatcaa ctctccgacc ttcgccatca aatccgattc gccggtcgcc ctccgcgccg
     1801 tcgcagggcc gagcgaggcg cttgactcac gctgctcgct cgtcaggcgg cgcaggctct
     1861 cgaccttttc aaatagctcg tctccggcct gttccttgag cattctgccg agcaagtgtc
     1921 caagtgagcg cacgtcgcgg cggagcggcg cttccttcaa ctccgggtca tcgctcgtta
     1981 attcggcaag gcgctccctc tgatcgctca ctttccataa agactctctc gacacttccg
     2041 cacctgcctt cccctaccgt gacccgtttc ggaatgcggt tgacctaaca
//
LOCUS       DASVES010000186         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1384850, whole genome shotgun sequence.
ACCESSION   DASVES010000186 DASVES010000000
VERSION     DASVES010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384850"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VF240_07435"
     CDS             complement(<1..926)
                     /locus_tag="VF240_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7175099.1"
                     /translation="MFRFQQEKTQLAPNFEAIRISLASPDKIRQWSHGEVTKPETINY
                     RTFKPERDGLFCARIFGPVTDWECLCGKYKRMKHRGVICDKCGVEVTQAKVRRERLGH
                     IELASPCSHVWFFKGLPSRIGHLLDIPLRELEKVLYFESYIVIDPGDAPVKEKELLTD
                     ERYRELMRDHPGAFVAKMGAEAIKELLAAVKIEELSGELRVRMKDETSQQKKLKFSKR
                     LKVVTSFLKSGNQPNWMILDVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNR
                     LKKLMELRAPEVIVRNEKRMLQEAVDALFDNGR"
     gene            complement(1115..4543)
                     /gene="rpoB"
                     /locus_tag="VF240_07440"
                     /pseudo
     CDS             complement(1115..4543)
                     /gene="rpoB"
                     /locus_tag="VF240_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          749 a         1487 c         1557 g          935 t
ORIGIN      
        1 cggccgttgt cgaagagtgc gtccaccgct tcctgaagca tgcgcttctc gttgcgcacg
       61 atgacctcgg gcgcgcgcag ctccatgagc ttcttgagac ggttgttgcg gttgatgacg
      121 cgccggtaga ggtcgttaag gtcagacgtg gcgaagcgtc cgccgtccag aggcacgagc
      181 gggcgaagct cgggcgggat gacgggaatc acgtcgagaa tcatccagtt gggctggttg
      241 cccgacttga ggaacgacgt gacgaccttc aggcgcttgg agaacttcag cttcttctgc
      301 tgcgaggtct cgtccttcat ccgcacgcgc agctcgcccg acagctcctc gatcttgacg
      361 gccgcgagta gttccttgat ggcctcggcc cccatcttgg cgacgaaggc gccggggtgg
      421 tcgcgcatca gctcgcggta acgctcgtcg gtgaggagtt ccttctcctt gacgggcgcg
      481 tcgccgggat cgatgacgat gtaagactcg aagtagagca ccttctcaag ctcccgcagg
      541 gggatgtcga ggaggtggcc gatgcgcgaa ggcaggccct tgaagaacca gacgtgcgag
      601 caggggctgg ccagctcgat gtggcccaga cgctcgcggc gcaccttggc ctgcgtgacc
      661 tcgacgccgc acttgtcgca gatcaccccg cggtgcttca tgcgcttgta cttgccgcac
      721 aggcactccc agtcagtgac cgggccgaag atgcgcgcgc agaacaggcc gtcgcgctcg
      781 ggcttgaagg tgcggtagtt gatcgtctcc ggcttggtga cctcgccgtg cgaccactgc
      841 ctgatcttgt cgggcgaggc gaggctgatg cggatggcct cgaagttcgg cgcgagctgc
      901 gtcttctctt gctggaatcg aaacatctta ttctccgaat gaaagtcagg agtcaggagt
      961 caggagtcag aatttaagag ccaccggcac agaagttttt cttctgactt ctggctcctg
     1021 actcctgtct ccctttgctt tattattccg cgctgctgac gacgacgggg cggcccacgc
     1081 cgtcttcttc gccctcggcc tccggcgctt tcttgtcgat cagctcaacg tcgaggcaaa
     1141 gcgactgaag ctctcgcacc aagacgttga acgactcggg gacgccgggg ttgaagtcgg
     1201 cctcgccctt gacgatggcc tcgtaaatct tcgagcggcc cgccacgtcg tccgacttgg
     1261 cggtcagaag ctcctgaagg atgtgcgccg cgccgtaggc ttcgagcgcc cacacctcca
     1321 tctcgccgaa acgctggccg ccgaactgcg ccttgccgcc gaggggctgc tgcgtgatga
     1381 gcgagtacgg cccgatggaa cgcgcgtgaa tcttgtcgtc caccagatgc gagagcttga
     1441 gcatgtagat gtagccgatg gtcacccgct gctcgaacgg ctcgcccgag agtccgtcgt
     1501 agaggatggt cttgcccgac tgatcgacga tctggggcaa gccctgctcg ctcaggcgct
     1561 cggccgcccg gccgatcatc tgcttgatct ccggctcggt cgcgccgtcg aagacgggcg
     1621 tggcgaagtg catcccgagc acgcgcgccg cccaccccag gtgcgtctcc agaatctgcc
     1681 cgacgttcat gcgcgagggc acgcccagcg ggttgaggac gatgtccacg ggtgtgccgt
     1741 caggcaggta gggcatgtcc tcctctggga ggatgcgggc gatgacgccc ttgttgccgt
     1801 ggcgaccggc catcttgtcg ccgacggaga gcttgcgctt catcgcgatg aagaccttga
     1861 ccatcttgat gacgccgggg gcgagttcgt cgccctgccg gagcttggcg atcttctcct
     1921 cgtagatgtc ggtgaggatg cgaatctgcc gctcggtgcg atcctcgaag tccttgacct
     1981 cggcggccac gtcggagccg gcccgcgccg aggcgagctg cgccttgcgg agctgctttg
     2041 gctcaagacc ggcgagcatc tcgcgcgtga tgacgatgcc cttctcgatg cgtacttcgc
     2101 gatccacgct caggtcggcc gagagcttgc ggccctcgaa cagctcgaag atgcgcgcgt
     2161 cgcgctgctc gcgcaggata cgcatttcgt cttcgaggtc gcgccggagg cggttctcct
     2221 cgtcctcctc gatggagagc gaacgctggt cgcgctcctg ccccttgcgc gtgaagacct
     2281 gcacctcgac gaccgtgccg tcgatgcccg gcgggcagac cagagaggcg tccttcacgt
     2341 cgccggcctt ctcgccgaag atggcgcgaa ggagtttctc ctcggccgtc agttgcgtct
     2401 cgcccttcgg cgtcaccttg ccgacgagga tcgagccggg cttgacctgc gcgccgatgc
     2461 ggatgatccc actgtcgtcg aggtcgcgga gcatgttctc gccgacgttc gggatgtcgc
     2521 gcgtgatctc ctcggggccg agcttcgtgt cgcgcgcctc gatctccagc tcctcgatgt
     2581 ggatcgacgt gtaatagtcg tccttcacca gtcgctcgga gacgaggatg gcgtcctcga
     2641 agttgtagcc gcgccacggc atgaaggcga ctaagacgtt gcgccccagc gccagctcgc
     2701 cgcggtcggt gcaggggccg tcggcgatga cctgcccttt gccgacgcgc tcgccgatgt
     2761 tgacgatggg gcgctggttg atgcaggtgt tctggttcga gcggcggaac ttcgtcagcg
     2821 tgtagatgtc ggccgtcacc tcgcgcgaga tggtgccgtc aacgttgtgg tcggcgcgga
     2881 cgatgacgcg ctccgaatcg acgtagtcca ccacgccgtc gcggcgggca gcgacgaccg
     2941 cgcccgagtc cttcgccgtc acctgctcca tccccgtgcc gatgtaggga gcctcggcgc
     3001 ggagcagggg cacggactgc cgctgcatgt tcgaccccat cagcgcgcgg ttcgcgtcgt
     3061 cgttctcaag gaacgggatg agcgaggcgg cgacggagac gagctgcttt ggcgatacgt
     3121 ccacgtactc gatgtcggcg cgcagggccg tcaggaactc gccggcctta cgcgcggcgt
     3181 tgcgctcgtt gacgatgtgg ccgtcctctt cgagttcgat gttggcctgt ccgatgacgt
     3241 gcttgtcctc gatccacgcc gtcaggtaga acggataagg ctcgtagtcg gccttcttcc
     3301 cgtcggccgc gacgcgcttg ttggccttga ggatgtcgtc ctcggtgatg tgctcgcccg
     3361 gcttgaactt cgtgtcgccg ccgttgacga tgcggacgta ctcgacgacc cgtccgtcgt
     3421 gtaccttgcg gtacggcgac tcgatgaagc cgaactcgtt gatgcgcgca aagcacgaca
     3481 gcgaggagat aagaccgatg ttcggccctt caggcgtctc aatcgggcag atgcggccgt
     3541 agtgcgtcgg gtgtacgtcg cgcacctcga agccggcgcg ctcgcgcgac aagccgcccg
     3601 gcccgagggc cgacagcctg cgcttgtgcg tgatctcgga gagcgggttc gtctgatcca
     3661 tgaactgcga gagctgcgag gagccgaaga actcacgaac ggcagccgtc acgggcttgg
     3721 cgttgatgag gtcgcgcggc atcgtcgtct gcatctcctg ctggatcgac atcttctcct
     3781 tgatggcgcg ctccatgcgg acgaggccga tgcggaactg gttctcaagg agttcgccca
     3841 ccgcccggac gcggcggttg ccgaggtggt cgatatcgtc ggccacgtac tcggcagggt
     3901 tgcgccgcat cttcaggaga tacgagatga cctgtacgaa gtcctgcgcc gagagcagcg
     3961 ggttgtcgat acgctcgcgc tccgggtgcc ccatcttgat gttgaacttc aaacggccga
     4021 cgcgcgagag gtcgaacttg cgctcgtcga agaacatgcc ctcgaagaga cggtaggccg
     4081 tctgcacggt cggcgggtcg ccgggccgca tcttgcggta gatttcgagg agcgcgtcca
     4141 cgggcttcga gatcacgtcc ttcttcaggg tctgcgagag cacgccgccc agatcgtcgc
     4201 gctcggggaa gaagacggtc agctcggtga cgcccgagtc gatgacctcc tgaagcttgg
     4261 cgggcgaaat ctcggagttg gcctcgacga cgacctcacc cgtctccatg ttgaccacgt
     4321 cgcccagagc gtatgcgcct tcgagctgct gcgaatcgac ctcgacctct ttgatgtgcg
     4381 ccttgcggat gtccttgatg gacgtttgcg tgatcttctt gccgccacgc accacaacgt
     4441 ctcccgagcg cgacttgatg tcgtgctcgg ccttcatccc ggccagattc gtcgctccgt
     4501 cttccttcac gcggacgaac agccgcccgc cctcggcgcg caccgtgtcg gggatgtaga
     4561 aggatgtaag gatgtcctcg gccgagaacg acgcgttctt gacggcctct tccagaagcg
     4621 agtcggaggc gaagcccttc gggtggaaat tcgggttgag ccagatgccc agcgcgcgca
     4681 ggaagatcga cgcgaggaac ttccgcttgc ggtcgatgcg gacgtaca
//
LOCUS       DASVES010000187         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_585179, whole genome shotgun sequence.
ACCESSION   DASVES010000187 DASVES010000000
VERSION     DASVES010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585179"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VF240_07445"
     CDS             <1..696
                     /locus_tag="VF240_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175100.1"
                     /translation="LKLVPPADGSEAVPSAPFADDEPPFPSDGAVRASAKSRAAAAHT
                     TEPPPFEARAPQTSGDDFAGDRQLPHADDGEWPRHEARGEADGGGASSGSSSGDPLER
                     IKRGLDARRKPFLAVAIEEATTARVNGEELFVAFTPDKKHLRDTLAKPDNVRLLQDVC
                     REALGHNIGVRIVIREADEGEEAPPTRDEEERRERQRLRELAEQQPAVQEVLRAFRAE
                     IVEVRRVDGEAPK"
     gene            872..1081
                     /locus_tag="VF240_07450"
     CDS             872..1081
                     /locus_tag="VF240_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175101.1"
                     /translation="MREPLLAGFLSLLIPGVGQIYNGQIISGIIWLVLTGVSWIGSAG
                     MLGWLVHLIAAYFAYSYAKEHPVRI"
     gene            1181..3157
                     /locus_tag="VF240_07455"
     CDS             1181..3157
                     /locus_tag="VF240_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX7175102.1"
                     /translation="MLRNSLPDTSGLAPSQTPSKKKPVQRLVPRIVATSLCLLLPILA
                     AAASPRVRAGEDTGVWTRVTSKNFTLVGDAEEAEMRRMARRLEEFRQALARLFPDFSV
                     DSHAPTTVVVFKSYEPYEGFRPLGAASGYFKAGEDSNYITLTANRRDEGDALTTLRHE
                     YVHLVVNDNLPHAPLWLSEGLAEVYAALEVTGDRRLTVGRALDQHARVLQRAQNLLPL
                     ATLLGVEHSSPHYGESSKREVFYAQSWALVHYLLFRRDGRQGVAELRRFTESLGRGQT
                     AAESLREAFGATPGEIEGGLSFYVGRAAFPSQTFVFGERLKFDDETRAEKLSAAETSA
                     RLGDLLLHLDRQTEAETHLRRALALDARLASARASLGMLRLKQNRLEEARAEFAAAVA
                     LGAADHLAHYHYALSIIRARAGTNASGGYLPETESLLRAQLLRSIELAPRYADAYDLL
                     AHVELSAGDLERAAALLKRAAELAPARADRYSLTLAQVHAHRGDLYSARRLLGAVARE
                     SSEAALRTRAAELLKAVDRQEESESLGAGADGREREDIVPEPPSVYSVRAKAEGEEQA
                     RGDLVTIECAAEEVFLHLLVEGRTLRFHARNFERLPFVSYDPAMARGRDRKLTCGTRA
                     AANHVLLTYRPASDAGLKAAGEVVAVDFVPREWN"
     gene            complement(3257..3790)
                     /locus_tag="VF240_07460"
     CDS             complement(3257..3790)
                     /locus_tag="VF240_07460"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7175103.1"
                     /translation="MASYCEFAVTDSLHKPYHDYEYGFPISDDSALFERLILEINQAG
                     LSWSTILRKRPAFRQAYGGFDIDVVAAYDEAERTRLLGDAGIIRNRLKVNAAIENARR
                     LIEVRRRYGSFAKWLDQHHPLTKEEWVKLFKQHFLFTGKEIVNEFLMSTGYLPGAHER
                     DCPVYERVVASNPHWLR"
BASE COUNT          679 a         1349 c         1179 g          624 t
ORIGIN      
        1 ttgaagctcg tcccgcccgc cgacggctcc gaggctgttc cttccgcccc tttcgccgac
       61 gacgagccgc cgttcccctc ggacggcgcg gtgcgcgcct ccgcgaagtc gcgcgccgcc
      121 gccgcgcaca ccaccgagcc gcctcccttt gaggcgcgcg ctccgcagac gagcggcgac
      181 gacttcgcgg gcgaccgtca actcccacat gccgacgacg gcgagtggcc ccgccacgag
      241 gcgcgcggcg aggcggacgg tggcggcgcc agcagcggca gtagtagcgg cgacccgctc
      301 gaacgcatca agcgcggtct ggacgcgcga cgcaagccgt tcctcgccgt ggcgattgag
      361 gaggcgacga cggcgcgcgt caacggcgaa gagttgttcg tcgccttcac gcccgacaag
      421 aagcacctgc gcgacacgct cgccaagccg gacaacgtgc gtctccttca ggatgtctgc
      481 cgtgaggcgc tgggacacaa catcggcgtc cgcatcgtca tcagggaagc ggacgagggc
      541 gaagaggcgc cgccgacaag agacgaggag gagcgacgcg agcgccagcg cctgcgcgag
      601 ctggccgagc aacagccggc cgtgcaggag gtgctgcgtg ccttccgcgc cgagatcgtc
      661 gaagtgcggc gtgtagacgg cgaagcgccg aagtaggagt caggagtcag gagtcaggag
      721 tcagaatgtt tcttcctgac ttgcgcgcga ggtccaatgt atcgccttcc cctgatttta
      781 ttctgactcc tgactcctga ctcctgacca cttcgctttc gtccgaaccg cccacggcct
      841 ccgcgcgtat agacccacgg aggaaatgaa catgcgcgaa ccgttactgg caggatttct
      901 cagtctctta attcccggcg ttggccagat ttataacggc cagatcatct cgggcatcat
      961 ctggctggtg ctgaccggcg tctcctggat cggctcggcc ggaatgctcg gctggctcgt
     1021 tcacctcatc gcggcctact tcgcctacag ctacgccaaa gagcaccccg tccgcatcta
     1081 gcacaaaact ttcagcggcg acgacaagcc gacgaacgaa ggggcgcagg cgattaacgc
     1141 ttgcgcccct ttctgttgac accggagcgc ggccccgcct atgctccgca acagtctccc
     1201 cgacacgtcc ggtctggcgc cctcgcaaac gccgtcgaag aagaagcctg tgcaacgtct
     1261 cgtcccccga atcgtcgcca cgtcgctctg cctcctgctg ccgatactcg ccgccgccgc
     1321 gtcgccgcgc gttcgcgccg gtgaagacac gggcgtctgg acgcgcgtca cctcgaagaa
     1381 cttcacactc gtgggcgacg ccgaagaggc cgagatgcgg cgcatggccc ggcgactcga
     1441 agagttccga caggcgctcg cgcgtctgtt cccggacttc agcgttgact cccacgcccc
     1501 gacgaccgtc gtcgtcttca agagctacga accctacgag ggcttccgcc cactcggcgc
     1561 cgcctccggc tacttcaagg ccggcgagga cagcaactac atcacgctga ccgcgaaccg
     1621 ccgcgacgaa ggcgacgcgc tcacgaccct tcgacacgag tacgtccacc tcgtcgtcaa
     1681 cgacaacctg ccgcacgcgc cgctctggct gagcgagggg ctggcggagg tctacgccgc
     1741 gctcgaagtc accggcgaca gacgcctgac ggtgggccgc gccctcgacc aacacgcgcg
     1801 cgtcctccag cgcgcgcaaa accttttgcc acttgcgacg ctgctcggcg tcgaacactc
     1861 ttcgccgcac tacggcgagt cgtcgaagcg cgaagtcttc tacgcgcagt cgtgggcgct
     1921 cgtccactat cttcttttcc ggcgcgatgg ccggcagggc gtggcggagc tgcggcgctt
     1981 tacggagtcg ctcgggcgcg ggcagaccgc cgccgagagc ctgcgtgagg cgttcggggc
     2041 cacgcccgga gagatcgagg gcggacttag tttctacgtt ggacgcgccg ccttcccgtc
     2101 gcagactttc gtcttcggcg agcgcctgaa gttcgacgac gagacgcgcg ccgaaaaact
     2161 ttccgcggcg gaaaccagcg cgcgcctcgg agacctgctc ctccatctcg accgacagac
     2221 ggaggccgag acgcacctcc ggcgcgcgct cgcgctcgac gcgcgccttg cgtccgcacg
     2281 cgcctcgctc gggatgctgc gtctcaaaca gaaccgcctc gaagaggcgc gcgccgagtt
     2341 cgccgcggcc gtcgcgctcg gcgcggccga ccacctcgcg cactatcact acgcgctctc
     2401 gatcatccgc gcccgcgccg gcacgaacgc gagcggcggc tacctgccgg agacggaaag
     2461 tctgctccgc gcgcaactcc tgcgctccat cgaactcgcg ccccgttacg ccgacgccta
     2521 cgacctcctc gcgcacgtcg aactctcagc gggcgacctc gaacgggccg ccgcgctcct
     2581 caagcgcgcc gccgaactcg cgcccgcgcg cgccgaccgc tactcactca cgctcgcgca
     2641 agtccacgcg caccgcggcg acctctacag cgcgcgccgt ctgctgggcg ccgtcgcgcg
     2701 cgaaagctcc gaggctgcgc ttcgcacgcg ggccgccgag ttgctgaagg ccgttgacag
     2761 gcaggaggag tcggagagcc ttggcgcggg cgcggacggc cgtgaaaggg aagacattgt
     2821 gccggagccg ccttcggtct attcggtcag ggcgaaggcg gagggtgagg agcaggcgcg
     2881 cggcgacttg gtcaccatcg agtgcgcggc ggaagaagtg ttcctgcacc tcctcgtgga
     2941 ggggcgcacg ctgcggttcc acgcgcgcaa cttcgagcgc ctgcccttcg tcagctacga
     3001 cccggcgatg gcccgcggcc gcgaccgcaa gctcacctgc ggcacgcgcg ccgcggccaa
     3061 ccacgtcctg ctcacctacc gcccggcgtc ggacgcgggc ctcaaggcgg ccggcgaagt
     3121 ggtcgccgtc gatttcgttc cgagagagtg gaattaaaat taaggcgtgc taactggcgg
     3181 ccggctgcgc ggcgtcgtca gacgcggccg tcttcttcaa ctcatacgac tggtcgcgca
     3241 cgaccaccgt ctccggtcat cgcagccagt gcggattgga cgccacgact ctttcataaa
     3301 cgggacaatc tcgctcgtgc gcccccggca gataccctgt gctcatcagg aactcattaa
     3361 cgatctcttt gcccgtaaat aaaaagtgct gcttgaacag cttcacccac tcttctttag
     3421 ttaacgggtg atgttgatcc aaccacttcg cgaacgagcc gtaccggcga cgcacttcaa
     3481 tcagccggcg ggcgttctcg attgcggcat tcaccttcag acgattgcgg atgatgcccg
     3541 cgtcacccag cagacgagtt ctctcggcct catcgtaggc agccaccaca tcaatgtcga
     3601 atccgccata cgcctgacgg aaggccggcc gcttcctcag aatggttgac caggaaagac
     3661 cggcttgatt gatttccaga atcaaccgct cgaacagtgc ggagtcgtcg ctgatgggga
     3721 acccatactc gtaatcgtga tacggtttgt gcaaagaatc cgtgacggcg aattcgcaat
     3781 aagaagccat agtttattga gtttagtcca gccccgattc aacaacctaa c
//
LOCUS       DASVES010000188        18571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1171515, whole genome shotgun sequence.
ACCESSION   DASVES010000188 DASVES010000000
VERSION     DASVES010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18571
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171515"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VF240_07465"
     CDS             <1..334
                     /locus_tag="VF240_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7175104.1"
                     /translation="TFSDTDIAVYRAALRQPGALTAAINYYRANVFSLFSGGGEERRW
                     AETRVSVPTLFIYGERDPFVIPETVRGISDFIDAPYREVRLARAGHWVQQEYPQEVNA
                     ALASFLEE"
     gene            485..1249
                     /locus_tag="VF240_07470"
     CDS             485..1249
                     /locus_tag="VF240_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7175105.1"
                     /translation="MDESSPILYAVEGAVALITLNRPDKRNALNDALVGGLKEALGEA
                     DADEAVRAVVITGAGADFCSGADLASLQKIAESSIVENLDDARSLMELFALIRRVRVP
                     VVAAVRGRALAGGCGLATACDIVLAARSARFGYPEVRIGFVPAMVMAILRRNVSEKRA
                     FELATRGAEIGAEEAERVGLVNRIFDDATFEEEVESYVAGFERVSRSAVMLTKRLLYQ
                     MDGMSFDAALQSGVEVNAIARMTEDCQRGIARFLKK"
     gene            complement(1287..1901)
                     /locus_tag="VF240_07475"
     CDS             complement(1287..1901)
                     /locus_tag="VF240_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7175106.1"
                     /translation="MSDETTNSAPPVIVEERDSIAIVRLNRPAERNSLTTPLLERLTH
                     IVSSLRVRSEISAIVFTGTADVFASGADIRELSGLTPEGARVFALRGQRLMRMIAEAG
                     QMTLAAIDGYCMGGGLDLALACDVRFASPGSIFAHPGARLGIITGWGGTQRLHRLIGT
                     TRAVELFLTTERVGGREALEMGLVDYLSDSPLDRALNVARDARR"
     gene            complement(1905..2309)
                     /locus_tag="VF240_07480"
     CDS             complement(1905..2309)
                     /locus_tag="VF240_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX7175107.1"
                     /translation="MSERKIRVLVAKPGLDGHDRGAKIIARALRDAGMEVVYTGLRQT
                     PEMIAAAALQEDVDAVGISILSGAHNTLCPRIVNLLRGQGMDDCLVLVGGIVPQEDIP
                     RLKEQGVAEIFLPGTSTEDIVKFLREKVQPRT"
     gene            complement(2393..4075)
                     /locus_tag="VF240_07485"
     CDS             complement(2393..4075)
                     /locus_tag="VF240_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7175108.1"
                     /translation="MPETKEIATPEQASDERWERETLEPVLRKRPERKKRFETVSLDE
                     VERLHTPADIADVDFERDISYPGEFPYTRGIHPTGYRGKLWTMRQFAGFGTPEETNAR
                     FKYLLEHGQTGLSVAYDLPALMGYDADSPLSEGEVGKCGVAVSSLADMEVLFDGIPLE
                     EVTVSQTINAPASVLLAMYLVVAEKQGADFRKISGTLQNDILKEYIAQKEWIYPIRPA
                     MKLVIDTFEFCTQHVPKYNPISVSGYHIREAGSTALQELAFTLRDGIEYVDWGVRAGL
                     DVDVFVPRISFFFNAHNDFFEEIAKYRAARRIWARVMRERFGSRSERTLQLRFHTQTA
                     GVSLTVQQPLNNIVRVAIQALAGVLGGTQSLHTDAYDEALALPTDRAALIALRTQQII
                     AEETGVVHTVDPLGGSYFIESLTKKMEDGALDYFEKIDAMGGMVEAIEKGFPQREIQE
                     SAYLYQKAVERGEQTIVGVNKYQMESEPGEVPTLVINEAVREHQLQRLEQTRAHRDEG
                     AVANSLEKVRRAAADGDNTMPATIEAVRAYATLGEICSALRDVYGVYEEPAF"
     gene            complement(4384..4809)
                     /locus_tag="VF240_07490"
     CDS             complement(4384..4809)
                     /locus_tag="VF240_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7175109.1"
                     /translation="MNKAANTYWTDARLAAQRPNARALTLPPPARPASDDAPALAPAW
                     VASRRNTFSVPSWVFFCMVVFATFAVCVTVTMRTHAEMRNAEQKFQSVGADVERLQGT
                     NDALRREVERLRSDPRAIEAAARTRLNMVRANEVVMPVN"
     gene            complement(4778..6187)
                     /locus_tag="VF240_07495"
     CDS             complement(4778..6187)
                     /locus_tag="VF240_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEX7175110.1"
                     /translation="MAIRFGTSGWRAIIADEFTFANVRLVTKSICEYLSSAGNAAGSP
                     LIVGFDTRFLGEQFAEQSVKVINSCGFNALLCDQPTPTPTISHAIKDRRAAGAINFTA
                     SHNPPEYNGMKFSTADGAPALPEVTKEIEERIGQLDAAQVDAAAGKKTTSEFEVYDPR
                     PTYFADLEAKVRFDTIAKAGGRYAFDPLWGTGRGYLDTILRAHDVEVETIHDWRDVLF
                     GGRSPEPGEEHVDELREVVKRNKCALGLATDGDADRFGVIDSDGAFIAPNNLIALLFD
                     YLAESRGWTGGVARSVATSHLVDRVAESRGLPVYETPVGFKFIGELINQDKIILGGEE
                     SAGLSMRGHYPEKDGILACLLAAEAVAARGASLGEQLEEIYGRVGRLEGKRVGVRLTP
                     ELLDSLPGRLAREPEEIGGRRVERVNRMDGVKFIFADGSWLLMRPSGTEPLVRIYAES
                     ESESDLEVLLEQGRKYLLD"
     gene            complement(6669..6956)
                     /locus_tag="VF240_07500"
     CDS             complement(6669..6956)
                     /locus_tag="VF240_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7175111.1"
                     /translation="MLAILGWIVLGLIAGLIAKAIMPGKDPGGVIVTILLGIVGAVIG
                     GWIGSALNIGGRSDNATDPGFFVSLIFAVIGALVVLAVYRLVTGRSLRAQN"
     gene            7468..8946
                     /locus_tag="VF240_07505"
     CDS             7468..8946
                     /locus_tag="VF240_07505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7175112.1"
                     /translation="MPLASMVRALCKASATGSDVLYDVMLKVALMVDATAQTYGLATF
                     AQKSGERPRLKWVEGLTQEEIVEAESRVIGALADEGLVEVKSGDREICLALSVATTQR
                     EGAAIYGRYVRPLSEKQACELRIISDVARLAHTHAQLREDLHVPARVPEQAVVTTAAT
                     LPGMVFVSRSMAELAHAVERVKDSESTVLITGESGTGKELIARAVHRLSRRSQAPFIP
                     FNCTAAPSDLIESMLFGHRKGAFTGAHADSEGLVRAAEGGTLFLDEIGDLPLGLQPKL
                     LRFLQEGEVHVLGERAPRQVNVRVVAATHKELLRGVSENTFREDLYYRLASLSLHVLP
                     LRERPEDIAALISHFLTHYARRNDRPIAGITAEAISVLQSYAWPGNARELAAEIERLV
                     LYTDEGAYIGVEHISPRIRPAGSGASEGVAPAAADLERLMEDFERRVITETLKRNDYN
                     VARAATALGLGSRQTLYKKLKRLAINVGDFLQDEQHPGLQLR"
     gene            9148..9642
                     /locus_tag="VF240_07510"
     CDS             9148..9642
                     /locus_tag="VF240_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7175113.1"
                     /translation="MILVYVLCFAVAMLIHELGHLVAARACMVPAGELGLGWGPKVFG
                     FRAGGVEYRLCAVPLGAYVRLDMAELQRRPLVQQVVVLLAGVIVNLVAAALAEGTAFG
                     TMNLLLAATNLLPLYQQDGWKCGMVMLRALLERRSPVVEWTFTIAGGLLSCVVFIAHA
                     TSRL"
     gene            10069..10344
                     /locus_tag="VF240_07515"
     CDS             10069..10344
                     /locus_tag="VF240_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175114.1"
                     /translation="MMKVVNNKTFVPVAGIALVVPFVFAAAYVGARAVGGAQADSGQG
                     LLLLCLALVGVLLGAVAGAGRQKAGEARESNALHITERERGMRKAFM"
     gene            10453..10623
                     /locus_tag="VF240_07520"
     CDS             10453..10623
                     /locus_tag="VF240_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175115.1"
                     /translation="MLKRFFCDETGLELSEYAVAAALVAIATVVAFETLGSNIGSKIT
                     ELGDVILNGSGQ"
     gene            10793..11320
                     /locus_tag="VF240_07525"
     CDS             10793..11320
                     /locus_tag="VF240_07525"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX7175116.1"
                     /translation="MQEHLTTAVAAILIPLAFIIAYYDVRYRRIPNAFVLAGLAGGLF
                     VNALAGGFQGLLMSVAGCLAAFAMMLVLHIFGAMGAGDVKLFAAIGSVVGLKLVAPTF
                     FVVVLTGGVLAVVSMLRAGNVGATMRRVLHIFAGLLPGWPMPRFAVPADKRHTIPYGV
                     AITLGSLISVVLFRA"
     gene            11373..12293
                     /gene="cpaB"
                     /locus_tag="VF240_07530"
     CDS             11373..12293
                     /gene="cpaB"
                     /locus_tag="VF240_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX7175117.1"
                     /translation="MRNKRLIVVLAGAVVFGVIAAVSVSRYLSNAQGFTRGMNKVVVA
                     KVEIPLGESIVAEQLTTASFPADGTPDGVFDSPEKLIGRVAVVQIAAREPVTDFKLAA
                     EGAQGGLGATIPEGYRAMSVKVDDVVGVAGFIKPGAMVDVLTVIEPSREQGARKDAVS
                     KIVLQNVKVLSNGQNIDKPKNEREAESVRVVTLLVTPEQAEKLALAAVEGKLRLVMRN
                     TIDQGDEKTPGADKNSLLTGEAAKPAPEPGALKSEQRSTPAAPRPVFHQVKRASAPAA
                     PKVEAAPAPAPPRPSAEMIQGSKRSIVEFP"
     gene            12449..14065
                     /locus_tag="VF240_07535"
     CDS             12449..14065
                     /locus_tag="VF240_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX7175118.1"
                     /translation="MHKRSPIARIAGLFGAALLMCLQAQAALGQQSSSVSASFGQSQA
                     EPVQINVLVGQSRVINFDRQIGRFSVSNPEVAEAVLVAPDQVLVNGKAFGQVNFIAWE
                     KSGGQFVVFDVYVRANLSLIDSQMRALFPGEDVRLSQANGSVVMSGKVKDMKTSTQAE
                     AVVAAAGFKTVNMMETPTQDMTQIQLQVRVAEVSRNKMKELAPSYGFTHGTTASYINS
                     GNSPTQLSNYSLSNRGSAIETLLAPSLNLFLGNGIAGVSGLLRALNSVGAVRSLAEPN
                     LIAMNGQQASFLAGGEFPVPMVQSSGDKIAVAVVWKEYGVRLNFKPTIIDEDHIRLEL
                     EPEVSTIDFANGVKLDGFLIPALKTRRAKTGVELRDGQSFALAGLLDNNEMQTFSKVP
                     GLGDIPILGHLFRSKSFTKQETELMFIVTAHMVKPMNRDELPEMRGIDGLKKGSPLGI
                     EQKGEGIKGDHGFETGTGATSSATPAAPKSDAPAAVPAAAPQKEEPKKDAVKTEDTSS
                     AAATQCELPTASALPVALSKLSFTAPRSQR"
     gene            14211..15356
                     /locus_tag="VF240_07540"
     CDS             14211..15356
                     /locus_tag="VF240_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX7175119.1"
                     /translation="MNGSHASDLQRRSERGSVLAISAFGMLAFLLAVGLALDVSHFYV
                     VKAELANAADAASLAGASALNSSTSGITMAADRAVTVMNNFEFDNTGVTINRSDVKFA
                     VNFAGPYLSESDAMAQAESIRFVHVDVPPKNVSVAFAQLTTGKDEVQMSHRAVAGMSM
                     SPNVFCEWVPLSVIDVPMVPKSTYTIRSPPGGFVSPGNYQILAVAGRGGKDVKYGLAG
                     GVNECAEPGTWYDVDTKPGVSSGPVRDGLNARFDDYAGGLDPNDYPPDVNVKENITWE
                     DYKLSLKTETRNADNFDAPNPDHPGVPYRRVILIPIIKLDEFDQGRDRVKFDRFAAFF
                     MQTKVQGGNGGEIKAEYIGERFMFGKGGYKPGGGPVTPELTQPVLYK"
     gene            15374..15871
                     /locus_tag="VF240_07545"
     CDS             15374..15871
                     /locus_tag="VF240_07545"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX7175120.1"
                     /translation="MRFFKSLLKTTPIRFARCERGTQLVELAIVLPVLLTLLATTAEF
                     ARFFHTYSTLSKATRSAARYLTTAPVDGSDDEKARNLVVYGSTVAEGDPLVHGMTTLQ
                     VNISREGGVASAGMYERVTVEIDGYNYQPIFDPGAFIGNDNASLNVEVSPKTTMRFMT
                     TTAGS"
     gene            15921..16574
                     /locus_tag="VF240_07550"
     CDS             15921..16574
                     /locus_tag="VF240_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX7175121.1"
                     /translation="MRVETSQRRSERGAALVEFTLGAVVFLTATFGVIEVSRLLWVHN
                     ALSDATRRAARFAVINPTTDVASVKNVAVYGNPEGEGKKLVGELEAADIDVVYKVSPI
                     TEVFGYPGGEVTVSVVDFEFTLNVPFIGMEIDLPDYRTTLTAESAGQIPEDITEPPDP
                     TPTPTPEPTPEPTPAPTPTPAPTPAPTPDPTPAPTPEPTPPSCPCGKKGNGDCKPCH"
     gene            16637..17893
                     /locus_tag="VF240_07555"
     CDS             16637..17893
                     /locus_tag="VF240_07555"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7175122.1"
                     /translation="MTQQLTFVILSTGLETFKELRGALMAEPDARILAGGDDAEQLLG
                     EIVRLKPSAAIVSLGPSPDAGLKFVERAAAECPRTVIICASRDASPDLILRSLRAGAR
                     EFLRLPVIAEEFKTVIARTAEFHAGQAEPPKKRGRTVAVFSNKGGCGTTFIAANLAAS
                     LQAPTVLVDLNLQSGDLGLFLGVEPKFSISDLVENRERVDDSLLRSYLAPHSANLSLL
                     SAPREADSADDIEPEHVFEVIEILRERFDYVVIDPQHTFDAITLAALDQADDVLLVLT
                     LDIPAIRCAQRALAIFDRLGYPRRKVQVVVNRWSKQIDLDLREVERYLGQKVTAYVQS
                     DYRMAVNSINLGQPLVEADPSSKIAMELRQIATSVCGPSAAPVPVAPQKRRFLGSLLR
                     RPQPQDEPQLIVKEKETFSPQQSAVS"
     gene            18068..>18571
                     /locus_tag="VF240_07560"
     CDS             18068..>18571
                     /locus_tag="VF240_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7175123.1"
                     /translation="MALRERLVRETPGAAAFFGRGGGDEAAGREGANLFQEMKSRLHR
                     AIINRMDLTKLGQLEPEQLHAEVSRLAEDIITSENAPLSTIDRERLVGEVRHELFGLG
                     PLEPLLADSAVCDILVNSPKNIYIERGGKLERTNVEFKDDEHLMRVIERIVSMVGRRI
                     DESSPMVD"
BASE COUNT         3451 a         5934 c         5924 g         3262 t
ORIGIN      
        1 caccttcagc gacaccgaca tcgccgtcta tcgcgcggcg ctccgccagc cgggcgcgct
       61 cacggcggcc atcaactact accgcgccaa cgtcttctcc ctcttctccg gcggcggcga
      121 ggagaggcgt tgggccgaga cgcgggtcag cgtccccacg ctcttcatct acggggagcg
      181 cgacccgttc gtcattcccg agacggtgcg cggcatctcc gacttcatcg acgcgcccta
      241 ccgcgaggtg cgcctcgcgc gcgcgggcca ctgggtgcag caggagtacc cgcaagaggt
      301 caacgcggcc ctcgcgagct ttctggaaga ataaaagtgg ttgacccgtc ggcttaagta
      361 cagtaccgcg agcggtagcg agcgggtgcg gctgtgatga gagattcgcc tcgcgcacgc
      421 gtgacccgct cgctaccgct cgcggtactg tttaaaacaa cggtcgatct cctcagtttc
      481 gcacatggat gaatcaagcc cgattctgta cgcggtcgag ggcgcggtcg cactcatcac
      541 gctcaaccgt cccgacaagc gcaacgcgct gaacgatgcg ctcgtcggcg gtctgaaaga
      601 ggcgctgggc gaggcggacg ccgacgaggc ggtgcgcgcc gtcgtcatca cgggcgccgg
      661 ggcggacttc tgctcgggcg ccgacctcgc ctcgctccag aaaatcgccg agagttcgat
      721 tgtcgaaaac ctcgacgacg cgcgcagcct gatggaattg ttcgctctca tccggcgcgt
      781 gcgtgtcccc gtcgtggcgg ccgtgcgtgg tcgtgcgctg gccggcggtt gcgggttggc
      841 cacggcctgc gacatcgtcc tcgccgcgcg ctccgcacgc ttcggctatc ccgaagtcag
      901 aatcggcttc gtgcccgcga tggtgatggc catcctgcgc cgcaacgtct cggagaagcg
      961 cgccttcgag ttggccacgc gcggcgcgga gatcggggcg gaggaggccg aacgtgtggg
     1021 actcgtcaac cgaattttcg acgacgcgac gttcgaggag gaagtcgaga gttacgtggc
     1081 gggcttcgag cgtgtcagcc gctcggccgt catgctcacc aagcgcctgc tctaccagat
     1141 ggacggaatg agcttcgacg cggcgctcca atcgggcgtc gaggtcaacg cgattgcgcg
     1201 catgaccgaa gactgtcaga ggggcatcgc ccgttttctg aagaagtgac aggactgttc
     1261 agtcgcgacc cacacgtcta cgactcttaa cgtcgcgcgt cgcgagcaac gttaagcgcc
     1321 cggtcgagcg gagaatccga caggtaatca accagcccca tctccagcgc ctctcgcccc
     1381 ccgacccgct cggtagtcag aaacaattcg acagcacgcg tcgtcccgat cagccggtga
     1441 agccgctgtg tgccgcccca cccggtgatg atgccgaggc gcgcgccggg gtgcgcgaag
     1501 atggagccgg gagaggcgaa gcgcacgtcg cacgcgagcg cgaggtcaag cccgccgccc
     1561 atgcagtagc cgtcgatggc cgccagcgtc atctgcccgg cctcggcaat cattcgcatc
     1621 agacgctggc cgcgcagggc gaacacgcgc gccccttcgg gcgtgagtcc gctaagttcg
     1681 cggatgtcag cgccggaggc aaacacgtcg gctgtgccgg tgaagacgat tgcggagatt
     1741 tccgagcgca cgcggaggga ggagacgatg tgggtcagac gttcgagcag gggagtcgtg
     1801 agcgagttac gctcggctgg acgattgagg cgaacgatgg cgattgagtc ccgttcttcg
     1861 acgatgacgg gcggggcgga atttgtggtc tcgtcgctca tcgctcaggt gcgcggctgc
     1921 accttctcgc gcaggaactt gacgatgtct tcggtcgaag tgccgggcaa aaagatttcc
     1981 gcgacgccct gctccttcag acgcgggatg tcttcctgcg ggacgatgcc gccgacgagg
     2041 acgagacagt cgtccatccc ctgtccgcgc aggagattca cgatgcgcgg gcatagtgta
     2101 ttgtgcgcgc cactcaagat ggagatgccg accgcgtcca cgtcttcctg caaggccgcc
     2161 gcggcgatca tctccggcgt ctgcctcagg cccgtgtaga cgacctccat cccggcgtcg
     2221 cgcaacgcgc gggcgatgat cttggccccg cggtcgtgac cgtcgaggcc gggcttggcg
     2281 acaagaacgc gaatctttct ttcagacata gaggtcagag gttagaggtt agaggtcagt
     2341 aaaagctgga ggccattcac tgacctctaa cctctaacct ctgacctctg ttttaaaacg
     2401 ctggctcctc gtagacgccg tacacgtcgc gcagggccga gcagatttcg ccgagcgtgg
     2461 cgtaggcgcg cacggcctcg atggtcgcgg gcatagtgtt gtcgccgtcc gcggctgcgc
     2521 ggcgaacttt ttcgagcgag ttggcgacgg cgccctcgtc gcggtgggcg cgtgtctgct
     2581 caaggcgctg gagctggtgc tcgcgcacgg cctcgttgat gacgagggtg ggcacctcgc
     2641 ccggctcgct ctccatctgg tacttgttga cgccgacgat ggtctgctcg ccgcgctcga
     2701 ccgctttctg gtaaagatag gccgactcct gaatctcgcg ctgcgggaag cccttctcga
     2761 tggcctcgac catgccgccc atcgcgtcga tcttctcgaa gtagtcgagc gcgccgtctt
     2821 ccatcttctt cgtcagcgat tcgatgaagt aggagccgcc gagcggatcg accgtgtgga
     2881 cgacacctgt ctcctcggcg atgatctgct gcgtgcgtag ggcgataagc gctgcgcggt
     2941 cggtcgggag cgcgagcgcc tcgtcatagg cgtcggtgtg caaactctgc gtgccgccga
     3001 ggacgccggc gagcgcctgg atggcgacgc ggacgatgtt gttaaggggc tgctggacgg
     3061 tgagggagac gcccgccgtc tgtgtgtgga agcggagctg caaggtgcgc tcgctacggg
     3121 agccgaagcg ttcgcgcatc acgcgcgccc agatacgacg cgcggcgcgg tacttggcga
     3181 tctcctcaaa gaagtcgttg tgggcgttga agaagaagct gatgcgcggc acgaacacgt
     3241 caacgtcgag gccggcgcgc actccccagt caacgtactc gatgccgtcg cgcagggtga
     3301 aggcgagttc ctgaagcgcc gtcgagccgg cctcgcggat gtgatagccg ctcaccgaaa
     3361 tcgggttgta cttcggcacg tgctgcgtgc agaactcgaa ggtgtcgatg acgagtttca
     3421 tcgccgggcg gatcgggtaa atccattcct tctgcgcgat gtactccttg aggatgtcgt
     3481 tctggagcgt gccggaaatc ttgcggaagt cggcgccctg cttctcggcg acgaccaagt
     3541 acatggcgag caaaacggac gcgggcgcgt tgatcgtctg cgagaccgtc acctcttcga
     3601 gcgggatgcc gtcgaagagc acttccatgt cggcgaggga agagacggcg acgccgcact
     3661 tgccgacctc gccttcggag agcggcgaat cggcgtcgta gcccatcaat gccggcagat
     3721 cataggcgac ggagagtccg gtctgcccat gttcgagcag atacttgaag cgcgcgttgg
     3781 tttcctcggg cgtgccgaag ccggcgaact ggcgcatcgt ccagagcttg ccgcgatagc
     3841 cggtggggtg tatgccgcgc gtgtagggga actcgccggg gtaggagatg tcgcgctcga
     3901 aatcgacatc ggcgatgtcg gcgggcgtgt ggaggcgttc gacctcatcg agcgagacgg
     3961 tctcgaaacg cttcttcctc tccgggcgtt tcctgagcac aggctccaga gtctcccgct
     4021 cccaacgctc gtcggacgcc tgctcgggcg tggcgatctc tttagtctca ggcatagttc
     4081 tctcgtcgct ccctgagcgc aaacgttgcg cggccgactt ctgagttctc tgtttatgct
     4141 acgagaaatc gatattagtt gaggcccgcg ggggtgtcaa ccgcttggtt tttcggcgtt
     4201 ccgggataaa gagcgcgagg agaaaaaggg cggcgcggcc cccggttttg ccggcggccg
     4261 cgcgcgtagg gcagggtcaa acttcggcgg cggatgatgc ggatgtggag cggcaggaga
     4321 ccggcagaaa ctgcaaatct tcggaggccc ttcctcgtcc gtcgtgggcg taagccatga
     4381 actttaattg acaggcatca cgacctcgtt ggcacgaacc atgttgagtc gcgtgcgtgc
     4441 ggcggcctcg atggcgcggg ggtcggagcg caggcgttcg acctcgcggc ggagcgcgtc
     4501 gttcgtgcct tgcagtcgct ccacgtccgc acccacgctc tgaaacttct gctcggcgtt
     4561 gcgcatctcg gcgtgagtac gcatcgtcac cgtgacgcag acggcgaagg tcgcgaacac
     4621 gaccatgcag aagaagaccc aggagggcac ggagaaggtg ttcctcctcg acgcgaccca
     4681 cgcgggggcg agcgccggcg cgtcgtccga ggcggggcgg gcgggcgggg gaagcgtgag
     4741 agcacgcgcg ttggggcgtt gcgcggcgag gcgcgcgtca gtccagtaag tatttgcggc
     4801 cttgttcaag gagcacctcc aggtcagact cggattcgga ttcagcataa atgcggacga
     4861 gcggctcggt gccggagggg cgcatcagca gccacgagcc gtccgcgaaa ataaatttca
     4921 caccatccat gcggttcacg cgctcgacgc gacgaccgcc gatctcttca ggttcgcgcg
     4981 ccagtctacc cggcagcgag tccaacaact cgggtgttag acgcacgccg acgcgcttgc
     5041 cttcaagccg cccgacgcgc ccgtaaatct cttcgagttg ttcacccaga ctcgcgccgc
     5101 gggccgcgac ggcctcggcc gcgagcaggc aggcgagtat gccgtctttc tccgggtagt
     5161 ggccccgcat cgagagtccg gccgactcct cgccgcccag gatgatcttg tcctgattga
     5221 tcaactcgcc gataaacttg aagcccaccg gggtctcgta aacgggaagc ccgcgcgact
     5281 cggccacacg gtcaaccaag tgcgaggtgg ccaccgagcg tgcgactccg cccgtccagc
     5341 cccgtgactc cgccagataa tcgaagagga gcgcgatcag gttgttgggg gcgatgaagg
     5401 cgccgtcgga gtcgatgacg ccaaagcggt cggcgtcgcc gtcggtcgca agccccagcg
     5461 cacacttgtt ccttttgacg acttcccgca gttcatccac gtgctcctcg cccggctccg
     5521 gcgagcgtcc gccgaaaagc acgtcgcgcc aatcgtggat cgtctcgacc tccacgtcgt
     5581 gtgcgcgcag gattgtgtca aggtagccgc ggcccgttcc ccatagcggg tcgaaggcgt
     5641 aacggccgcc ggctttggct atggtgtcga aacggacttt tgcctcaaga tcggcaaagt
     5701 atgtggggcg cggatcgtaa acttcaaatt ccgaggtggt tttcttgccg gcggccgcgt
     5761 ccacttgggc ggcatccagt tggccgatgc gctcctcgat ttctttcgtg acttcgggga
     5821 gcgccggtgc cccgtccgcc gttgaaaact tcatcccatt gtattcaggc ggattatggg
     5881 aagcagtgaa gttgattgcg cccgccgctc gacgatcttt aatggcatgc gaaatggtcg
     5941 gcgtaggcgt tggctggtca caaagtaggg cattgaatcc acatgagttg atgaccttca
     6001 cgctttgctc ggcaaactgt tcgcccagaa atcttgtgtc aaagcccaca atcaggggac
     6061 tacccgcagc atttccagcc gaactcagat attcgcagat cgacttcgtc accaaacgga
     6121 cgttggcaaa agtgaattcg tcggcgatga tagctcgcca gccggatgtt ccgaatcgaa
     6181 tcgccattgt tggttaaaaa aatcgctgat ttgagcttag aaacccaaca tacagtggcg
     6241 ttgaaaagaa gtgccacaac acaatgtgtg cttgaccacc tgcaatcctt tgtcgctcat
     6301 agacttagcg acaaagtggc gggaatgtaa catgggagcc ttggggtgta aagagttttc
     6361 ttttcaacgc tttatgcggc taagttcaac atatacatga ggtaagggtt tctaaattgt
     6421 ctcgatggcg aacatggggc gaacggaatt ccgccgcttg ggcattgatt taaaagggct
     6481 tgccgtgaca gatagatagg ggcttgatgt catcatcaca ggtgtggaaa ataatgaagc
     6541 cgccgtcgcg gtcaattcga gaggagtttt aaggggacat ggctttgaag tcagaggcta
     6601 aaaagtaacg cggggcctca aaaacatttg aagccccgcg ctaagttcgg taagtctaac
     6661 taaagaagtt agttctgagc cctcagactc ctgccggtga ccagtctgta gacggcgagc
     6721 acgaccaacg cccctattac agcgaaaatg aggctcacaa agaaaccggg atcggtcgcg
     6781 ttgtcgctcc tgcccccgat attgagagcg ctaccaatcc agccgccgat gacggcgccg
     6841 acaatcccca acaggatagt cacgatgacg ccaccggggt ctttgccggg cataattgcc
     6901 ttagcgatga gacctgctat caagccaaga acgatccaac caagtatagc taacatgcct
     6961 taaccctcct ctggtattag gcgcctgaaa gtattagaac ttgcagacgc cgcatcatgc
     7021 aaaaaaaccg aatgtaccgt ttcagtattc aaaatactga ttcgtcgcaa tgggtgcaat
     7081 actcaagcca cagctgactt gagtccattt ccagaaccgg acgcatgagt aattgaaggc
     7141 aaatatggca aatgaatcag aaggaagagg acgagcggtt ggaatttagg atagccggac
     7201 ggtcgatttt ataattcagt tgacaggtta aacggctcct gtatagagtg agaatgtccg
     7261 cgtcgaagac accgctagtg gctttcggct ggctgacgcg atacggcccc gcccactgcc
     7321 gtcgttattc ttgccctgac atttacctct cacttttcgc atccggtttt tcccgctcag
     7381 gtctcatttc aaggggtttt ttgttttatg gacaacctgc tgcttccgtc tggagaagca
     7441 tgtgtcggag cagacggtgc atttcagatg ccgctcgctt cgatggtgcg cgccctgtgc
     7501 aaggcgtccg cgacgggttc cgacgttctc tacgacgtga tgctgaaggt cgcgctgatg
     7561 gttgacgcca cggcgcagac ctatgggctg gcgacgttcg cgcagaagtc tggggagcgg
     7621 ccgcgtctca agtgggtcga aggactcacg caggaggaga tagtcgaggc cgagtcgaga
     7681 gtcatcggcg cgttggcgga tgaaggcctg gtcgaggtca aatcgggaga ccgggaaatc
     7741 tgtctggccc tctcagtcgc cacgacgcag cgcgaaggcg cggcgatcta cggacgttac
     7801 gtccggccac tttctgaaaa gcaggcgtgc gagttgcgga tcatctccga tgtggcgcgg
     7861 ctcgcgcaca cgcacgcgca gttgcgcgaa gacctgcacg tcccggcccg tgtgccggag
     7921 caggcggtgg tgacgacggc ggcgacgctg ccgggcatgg tcttcgtgag tcgctcgatg
     7981 gcagagctgg cgcacgcggt cgagcgcgta aaggattccg aatcgaccgt gttgattacg
     8041 ggcgagtcgg gtacgggcaa ggagctgatc gcgcgggccg tccaccgtct gagccggcgc
     8101 agtcaggctc cgttcatccc cttcaactgc acggcggccc cgtccgacct gatcgagagc
     8161 atgttgttcg gccaccgcaa gggggccttc accggcgcgc acgccgacag cgaaggactg
     8221 gtgcgcgccg ccgagggcgg gacactcttc ctcgacgaga tcggcgacct gccgctcggg
     8281 ttgcagccga agctcctgcg cttccttcag gagggagagg ttcacgtgct cggtgagcgc
     8341 gcgccgcgtc aggtcaacgt gcgcgtcgtc gccgcgacgc acaaggaatt gttgaggggg
     8401 gttagtgaaa acactttccg cgaagacctg tactaccgcc tcgcctcgct ctctttgcac
     8461 gtgctgccgt tgcgcgagcg gcccgaggac atcgccgctc tcatctctca cttcctgacg
     8521 cactacgcgc gtcgcaacga ccgacccatc gccggcatca cggccgaggc catctcggtc
     8581 ttgcagagct acgcgtggcc cggcaacgcg agggaactcg cggccgagat cgagcgcctc
     8641 gtgctctaca cggacgaggg agcgtatatc ggcgtcgagc acatcagccc gcgcatccgc
     8701 ccggcgggca gcggggcgtc ggagggcgtc gcgccggccg cggccgacct cgagcgtctg
     8761 atggaggact tcgagcggcg cgtgattacg gagacgctca agcgcaacga ctacaacgtg
     8821 gcgcgggccg ccacggcgct cggcctcggc tcgcgccaga cgctctacaa gaagctcaag
     8881 cggctggcga taaacgtcgg cgacttcttg caggacgaac agcatcccgg cttgcaactt
     8941 cgctgaacgg gccggacgtg gatgttcgac gtttgaaagt gcgcttgacg cggcacgggc
     9001 cggacagctt ccgcgatgat ttctgattcg gcttcctgac gagcaaccga taatcatttc
     9061 ttcgcccggt tccgacgcaa aaaatccttc ttcgctcgcg tccgacacgc ctcggtctaa
     9121 cgatcccaat caaaaccagt gccccttatg atcctcgttt acgtactctg tttcgcggtg
     9181 gccatgttga tacatgagct gggccatctc gtggcggcgc gcgcgtgcat ggtgccggcg
     9241 ggcgagcttg gactgggatg ggggccgaag gtttttggct tccgggcggg cggggtcgag
     9301 tacaggctgt gcgcggtgcc gctgggcgca tacgtgcgtc tggacatggc ggagttgcag
     9361 cgccgcccgc tcgtgcagca ggtggtcgtg ttgctcgcgg gcgtcatcgt caacctcgtc
     9421 gccgcggcgc tcgcggaagg gacggcgttc gggacgatga acctgctgct cgcggcgacg
     9481 aatctgctgc cgctctacca gcaggacggc tggaagtgcg gcatggtcat gctgcgggcg
     9541 ctgttggaac gacgcagccc cgtcgtcgaa tggacgttca ccatcgcggg cggcctcttg
     9601 agctgcgtcg tgttcatcgc ccacgcgacg agccgtctgt gaagtcgtcg gcatgagatt
     9661 cagtttacgc aggtagcatt caaccggaca tgatgccggc cgtccaaacg gggcggcccc
     9721 gcccgagaca gtttgtcggg agtggggccg ccccgaagtg tttgtagtcg cggcgtcacc
     9781 gccggttgat gtggcttagt tttctgtcgc ccgcggatga cagacggcta agcctttgaa
     9841 gtttcagggt tcacggcgcg gcgacgccga atgtggaaaa gggaagcgcc cccgcgttgt
     9901 aaaccgcggg cgacagacgc gaacggggcg cggggcgttg aaccgcgtgt ttgacgggcg
     9961 tttgtcgatt cccaccttgt ggcacgggcc ttgagataga aaggcgcgag ccgaggcgag
    10021 aacctgtcaa gtgacactgc ggggaatcga ccgaggccgc gagggaagat gatgaaagtc
    10081 gtgaacaaca aaacgttcgt acccgtagcc ggcatcgcgc tcgtcgtgcc tttcgtcttc
    10141 gccgccgcat acgtcggggc gagggcggtg ggtggagcgc aggcggacag cgggcaggga
    10201 ttactgctgt tgtgcctggc gctcgtcggt gtgctcctcg gggccgtcgc cggcgcgggc
    10261 cgtcagaagg cgggtgaggc gcgcgagtca aacgcgctcc acatcacgga gcgcgagcgc
    10321 gggatgcgca aggctttcat gtagacgccc ggtcacttcg aaccacccgg tcccgccgcc
    10381 gcctcgtcaa gggtcgaagc gcgaaggggc ttaagaccca aaaccccaac cacaacaaag
    10441 gagcaaacta caatgttgaa gagattcttc tgtgacgaaa ccggtctgga gctttccgag
    10501 tacgcagtcg ccgccgccct cgtcgccatc gcgacggtcg tggccttcga gaccctcggc
    10561 tcgaacatcg gttccaagat caccgaactc ggcgacgtga tcctgaacgg cagcggacag
    10621 taagacgagg ccgccaagaa atccgggtgg cgtgccgcgc catcgactcg accctcggtt
    10681 gagcaagtgc gcggcacgcc accgatttcc atcagcgggc gagcaactgc aacttcccgc
    10741 ctccttctga acgaagcact tcccgcgagc cggcacgaag ccggcaatct cgatgcaaga
    10801 acatctcaca actgcggtgg cggcgatact catcccgctg gcattcatca tcgcctatta
    10861 cgacgtgcgc taccgccgca tccccaacgc cttcgtgctc gccggcctcg ccggcggcct
    10921 gttcgtcaat gccctcgccg gtggatttca aggcttgctc atgagcgtcg cgggttgtct
    10981 cgcggctttc gcgatgatgc tcgtgctgca catcttcggc gcaatgggcg cgggcgacgt
    11041 gaagctgttc gcggccatcg ggtcggtcgt cggcctcaag ctggtggcgc cgaccttctt
    11101 cgtcgtcgtg ctgacgggcg gagtgctggc cgtcgtctcg atgctgcggg ccggaaacgt
    11161 gggcgcgacg atgcggcgcg tgctgcacat cttcgcggga ctgctgcccg gctggccgat
    11221 gccgcgcttc gccgtgccgg ccgacaagcg gcacacgatc ccctacggcg tggccatcac
    11281 gctcggcagt ttgatctcgg tagtgctgtt ccgcgcgtga gctgttgcgc gcgggtaatg
    11341 tttcgacccc catcctggag aattgagaag ccatgcggaa taaacgtctc atcgtggtgc
    11401 tggccggcgc ggtcgtcttc ggagtgatcg ccgccgtctc cgtcagccgc tacctgtcga
    11461 acgcgcaggg gttcacgcgc ggcatgaaca aggtggtcgt cgcgaaggtg gaaatccccc
    11521 tcggtgaatc catcgtcgcc gagcagttga cgacggcgag tttccccgcc gacggcacgc
    11581 ccgacggggt gttcgactcc cccgagaagc tcatcggccg cgtggccgtc gtccagatcg
    11641 ccgcgcgcga gccggtcacc gatttcaagc tcgcggctga gggcgcgcag ggcggactcg
    11701 gggccaccat ccccgaaggc taccgcgcga tgtccgtcaa ggtggacgac gtggtcggcg
    11761 tcgccggctt catcaagccg ggcgcgatgg ttgacgtgct gacggtcatc gagccgtcgc
    11821 gcgagcaggg cgcacgcaag gacgccgtct cgaagatcgt ccttcagaac gtcaaggtgc
    11881 tctccaacgg tcagaacatc gacaagccga agaacgagcg cgaggccgag tcggtgcgcg
    11941 tcgtgacgct tttggtgacg cccgagcagg cggagaagct ggcgctggcg gccgtcgagg
    12001 gcaagctccg gctcgtgatg cgcaacacca tcgatcaggg cgacgagaag acgccgggcg
    12061 ccgacaaaaa cagcctgctg acgggcgagg cggccaaacc cgcccccgag ccgggcgcgc
    12121 tcaagagcga gcaacgctcg acgcccgccg cgccgcgtcc cgtcttccat caggtgaagc
    12181 gcgcgagcgc gcccgccgcg ccgaaggtcg aggcggctcc ggcgcccgcg ccgccgcgcc
    12241 cctccgccga gatgattcaa ggctccaagc ggagcatcgt cgagtttcca tgagcgagct
    12301 tttagctatc agctattagc agtcagcccg acggcaaaag ccgttcatga cgcagccgct
    12361 gatgccgaag ccacacatta cccgctccct gttggctaac agctaaaagc taaccgctaa
    12421 cagctttttc aaaggagtca aaagagtcat gcacaagcgt tcccctatcg cacgcatcgc
    12481 gggattgttc ggcgcggcgc tcctgatgtg ccttcaggca caggcggcgc tcggtcaaca
    12541 gtcgtcttcg gtcagcgcct ccttcggaca gtcgcaggcc gagccggtgc agatcaacgt
    12601 gctggtcgga cagtcacgcg tcatcaactt cgaccgccag atcggccgct tctcggtgtc
    12661 gaaccccgag gtggccgagg ccgtcctcgt cgcccccgat caggtgctcg tcaacggcaa
    12721 ggcgttcggg caggtcaact tcatcgcgtg ggagaagtcg ggcgggcagt tcgtcgtctt
    12781 cgacgtgtat gtgcgcgcca acctctcgct catcgactcg cagatgcgcg ccctcttccc
    12841 cggcgaggac gtgcggctga gccaggccaa cggctcggtc gtgatgtcgg gcaaggtcaa
    12901 ggacatgaag acctcgacgc aggccgaggc ggtcgtggcc gccgccggct tcaagaccgt
    12961 caacatgatg gagacgccga cgcaggacat gacgcagatt cagcttcagg tgcgcgtggc
    13021 cgaggtgagc cgcaacaaga tgaaggagct ggcgccctct tacggcttca cgcacggcac
    13081 gacggccagc tacatcaaca gcggcaactc gccgacgcag ctcagcaact actcgctctc
    13141 gaaccgcggc agcgccattg agacgctgct cgcgccgtcg ctcaacctct tcctcggcaa
    13201 cggcatcgcc ggtgtctcgg ggttgctgcg cgccctcaac tcggtcggcg ccgttcgcag
    13261 tcttgccgag ccgaacctga tcgcgatgaa cgggcagcag gcgagcttcc tcgccggcgg
    13321 cgagttcccc gtgccgatgg tgcagagcag cggcgacaag atcgccgtcg ccgtcgtgtg
    13381 gaaggaatac ggcgtccgtc tcaacttcaa gccgacgatc atcgacgagg atcacatccg
    13441 tctggagctg gagccggaag tctcgaccat cgacttcgcc aacggcgtca agctcgacgg
    13501 cttcctcatc cccgccctca agacgcgccg cgccaagacg ggcgtcgagt tgcgcgacgg
    13561 ccagtcgttc gcgctcgccg gcctgctcga caacaacgag atgcagacct tctcgaaggt
    13621 gccgggcctc ggcgacatcc ccatcctcgg tcacctcttc cgctcgaagt cgttcacgaa
    13681 gcaggagacg gagctgatgt tcatcgtcac ggcgcacatg gtcaagccca tgaaccgcga
    13741 cgagctgccc gagatgcgcg gcatcgacgg actcaagaaa ggctcgccgc tcgggatcga
    13801 acagaagggc gagggcatca agggcgacca cggcttcgag accggcacgg gcgcgacctc
    13861 gtcggcgacg cccgccgcgc cgaagtcaga cgctccggcg gccgtgcccg ccgcggcccc
    13921 gcagaaagag gagccgaaga aggacgccgt gaagaccgaa gacaccagct cggcggccgc
    13981 cacacagtgt gaactcccca cggcctccgc gttacccgtg gcgctctcga agctcagctt
    14041 cacggcgccc agatcgcaga ggtaagggga cgcgggggcg cgccccaccg cacacggagc
    14101 gcgccccccg tcataacgga ggtgataagt gatgagtgat aagtgataag aaaaacctct
    14161 tatctcatca cttatcactt atcactcatc actcagagga gggtgaagag atgaacggat
    14221 cgcacgcttc agatttacag agacggtctg agcgcggctc agtcctcgcc atcagcgcgt
    14281 tcgggatgct cgcctttctc ctcgccgtcg gtctcgcgct cgacgtgagc cacttctacg
    14341 tcgtcaaggc ggaactcgcc aacgccgccg acgccgcttc acttgccggc gccagcgccc
    14401 tcaactcctc cacctccggc atcacgatgg ccgccgaccg cgccgtcacc gtgatgaaca
    14461 atttcgagtt cgacaacacg ggcgtcacca tcaaccgctc cgacgtcaag ttcgccgtca
    14521 acttcgccgg cccctacctg agcgagtcgg acgcgatggc gcaggcggag agcatccgct
    14581 tcgtccatgt ggacgtgccg ccgaagaacg tgagcgtcgc tttcgcgcag ctcacgacgg
    14641 gcaaggacga ggtgcagatg tcgcaccggg cggtggccgg catgagcatg tcgccgaacg
    14701 tcttctgcga atgggtgccg ctctccgtga tcgacgtgcc gatggtgccc aagagcacct
    14761 acaccatccg ctccccgccg ggtggcttcg tctcgccggg caactaccag attctcgccg
    14821 tggcgggacg cggcggcaaa gacgtgaagt acggcctcgc cggcggcgtc aacgagtgcg
    14881 ccgagccggg cacctggtac gacgtggaca cgaagccggg cgtctcctcc ggccccgtcc
    14941 gcgacggcct caacgcgcgc ttcgacgact acgcgggcgg cctcgacccc aacgactacc
    15001 cgccggacgt gaacgtcaaa gagaacatca cctgggaaga ttacaagctg agcctcaaga
    15061 ccgagacacg caacgccgac aacttcgacg cgccgaaccc cgatcacccc ggcgtgccct
    15121 accgccgcgt catcctcatc ccgatcatca agctcgatga gttcgatcag gggcgcgacc
    15181 gcgtgaagtt cgaccgcttc gccgccttct tcatgcagac gaaggtgcag ggcggcaacg
    15241 gcggtgagat caaggccgag tacatcggcg agcgcttcat gttcggcaag ggcggctaca
    15301 agcccggcgg cggccccgtc acgccggaac tcacccagcc cgtgctctac aagtgaacgc
    15361 ggagggagca gcgatgaggt tcttcaaaag tttactgaag acgacgccca tccgcttcgc
    15421 cagatgcgag cggggcacgc agctcgtcga gctggccatc gtgttgcccg tcctcctcac
    15481 gctgctggcg acgacggccg agttcgcccg cttcttccac acctactcga cgctgtcgaa
    15541 ggcgacgcgc tcggccgcgc gctatctgac cacggcgccc gtggacggca gcgacgacga
    15601 gaaggcccga aacctcgtcg tctacggcag caccgtggcc gagggcgacc cgctcgtcca
    15661 cgggatgacg accttgcagg tgaacatctc gcgcgagggc ggggttgcct cggccgggat
    15721 gtacgagcgc gtgaccgtcg agattgatgg ctacaactac cagcccatct tcgaccccgg
    15781 cgcgttcatc ggcaacgaca acgcctcgct caacgtcgag gtcagcccga agacgacgat
    15841 gcgttttatg acgacgaccg ccgggagttg attcgacgaa gcgggacgtg tcagcgatgg
    15901 agaccgcgga ggtgtgagcg atgagagttg agacgagcca gaggagaagt gagcgcggcg
    15961 cggccctcgt ggagttcacg ctgggcgccg tcgtcttcct cacggcgacc ttcggcgtca
    16021 tcgaggtctc gcgcctgctg tgggtccaca acgctctctc cgacgccacg cgccgcgccg
    16081 cccgcttcgc cgtcatcaac ccgaccacgg acgtggcgag cgtcaaaaac gtggccgtct
    16141 acggcaaccc cgagggcgag gggaagaagc tggtcggcga gcttgaggcg gccgacatcg
    16201 acgtggtgta caaggtgtcg ccgatcacgg aagtgttcgg ctaccccggc ggcgaggtga
    16261 cggtcagcgt cgtcgatttc gagttcacct tgaacgtccc gttcatcggt atggagattg
    16321 acctgcccga ctaccgcacg acgctcacgg ccgagagcgc cggccaaatc cccgaggaca
    16381 tcacagagcc tcccgacccg acgccgaccc cgacgccgga gccgacacct gagccgacgc
    16441 ctgcgccaac accaacaccg gccccgacgc ccgcgccgac gcctgatccg acgcctgcgc
    16501 ccacgcccga gccgaccccg ccgtcctgcc cgtgcggaaa gaaggggaac ggcgactgta
    16561 agccctgcca ttgagaaagg gcgcgcgcgg agcgagcaga gatagtttcc tacaactcga
    16621 cacgaacgaa gcgaccatga cccaacagct cacattcgtc atcctcagca ccggccttga
    16681 gaccttcaag gagctgcgcg gcgcgctgat ggccgagccg gacgcgcgca tcctcgccgg
    16741 cggcgacgat gccgagcaac tcctcggaga gatcgtgcgc ctcaagccct cggcggccat
    16801 cgtctccctc ggcccgtcgc ccgatgccgg cctcaagttc gtcgagcgcg ccgccgccga
    16861 gtgcccgcgc acagtaatca tctgcgcctc gcgcgacgcc tcgcccgatc tcatcctgcg
    16921 atctttgcgc gccggcgcgc gcgagttcct gcgcctcccc gtgatcgccg aggagttcaa
    16981 gacggtcatc gcgcgcacgg ccgagtttca tgccgggcag gccgagccgc cgaagaagcg
    17041 cgggcgcacc gtcgccgttt tctccaataa gggcggctgc ggcaccacct tcatcgccgc
    17101 caacctcgcc gcctcgctgc aagcgccgac cgtcctcgtc gatctcaacc tccagtcggg
    17161 cgacctcggc ctcttcctcg gcgtggagcc gaagttctcc atctccgacc tcgtcgagaa
    17221 ccgcgagcgc gttgacgact cacttctccg cagctacctt gcgccccact ccgcgaacct
    17281 ctcgctgctc tccgcgccgc gcgaggccga ctcggccgac gacatcgagc cggagcacgt
    17341 cttcgaggtc atcgaaattt tgcgcgagcg tttcgactac gtcgtcatcg acccgcagca
    17401 caccttcgac gccatcacgc tcgccgcgct cgatcaggcc gacgacgtgc tgctcgtcct
    17461 gacgctcgac atccccgcca tccgctgcgc ccagcgcgct ctcgccatct tcgaccgcct
    17521 cggctacccg cggcgcaagg tgcaggtcgt cgtcaatcgc tggtcgaagc agatcgacct
    17581 cgacctgcgc gaggtcgagc gatacctcgg gcagaaggtc acggcctacg tccagagcga
    17641 ctaccgcatg gccgtcaact ccatcaacct cgggcagccg ctcgtcgagg cagacccgtc
    17701 gtcgaagatc gcgatggagc tacggcaaat cgccacgagc gtctgcggcc ccagcgccgc
    17761 ccccgtcccc gtggccccgc agaagcgacg cttcctcggc tcactcctgc gccgcccgca
    17821 gccgcaggat gagccgcagt tgatcgtgaa agagaaagaa accttcagcc ctcagcagtc
    17881 ggcggtcagc taaaaagctt ttagcgttta gctgttagcg ttcagcaaaa aaaagcagcg
    17941 acagaaaaca acgaacaggg aagggagtcg gccggcggtt acagaaatag ttcaggcggc
    18001 acaggcgacg aagctaactg ctaaccgcta accgctaacg aaagctaacg gctgacagct
    18061 aacaaccatg gcacttcgcg agagactggt cagggagact ccgggcgcgg cggcgttctt
    18121 cgggcgcggc ggcggcgacg aggcggcggg gcgcgagggc gcgaacctct tccaggagat
    18181 gaagtcgcgc ctgcaccgcg ccatcatcaa ccgcatggac ttgaccaagc tcgggcagct
    18241 cgaacccgag cagctccacg ccgaggtctc ccgtctcgcc gaagacatca tcacgtccga
    18301 gaacgcgccg ctctccacca tcgaccgcga gcgactcgtc ggcgaggtgc gtcacgagct
    18361 gttcggcctc gggccgctgg agcctttgct tgccgactcg gccgtctgcg acatcctcgt
    18421 caactcgccg aagaacatct atatcgagcg cggcggcaaa ctcgaacgca ccaacgtcga
    18481 gttcaaagac gacgagcacc tgatgcgcgt catcgagcgc atcgtctcga tggtcggccg
    18541 ccgcatcgac gaatcctccc cgatggtgga c
//
LOCUS       DASVES010000189         7834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_234302, whole genome shotgun sequence.
ACCESSION   DASVES010000189 DASVES010000000
VERSION     DASVES010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234302"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VF240_07565"
     CDS             complement(<1..531)
                     /locus_tag="VF240_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175124.1"
                     /translation="MPPKNRQQRQIEKSHKATAAEHQPWFDRRIVLFVVALKLFMLVF
                     GVAAYAILTNQPAGSFQGWLELWNRWDVPHYIDIARDGYVATDLVSKDQRLWIVFYPL
                     YPWLLRLFGIVLRDYVLTFHVVSLIGAVAAGLLFYRLARLDLEDTSARAAVFFMFIFP
                     TAYFFHTGYTESVFMPL"
     gene            complement(641..2584)
                     /gene="asnB"
                     /locus_tag="VF240_07570"
     CDS             complement(641..2584)
                     /gene="asnB"
                     /locus_tag="VF240_07570"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7175125.1"
                     /translation="MCGIAGLISSEPERRIGAMLKAIEHRGRDDEGVWTSVAADGEGR
                     RACLGHRRLAIIDTSEGGHEPMLSADGRYVLTFNGEIYNFREVRAELVGRGRQFHTDS
                     DAEVLLAAFAEWGEECLPRLNGMFAFAVWDERERVLTVARDRLGIKPLFYATPQGDNG
                     AGTSFVFASEAKAILASGLVERALNVEGLNQQLTFLWTPDPHTLFKGVKKLPPGHVLT
                     WRDGRVSVREWWDVSFDELETGRGVEWWREQVLETFERVVRLEMVSDVPLGSFLSGGV
                     DSSMIVAMMKRHSEGRRVSTYTIGFEPEDLRYDIIPDDARWARRVGQLFDTDYHEIIL
                     QPDVAALLPKLVYHMESPLIDSVISSYLVARAARETLTVLLSGMGGDEVFAGYPRHLA
                     MALAGALDPIPSSLRRPIMRALAETLPGGMPGLLTAPLRNAKKFARSAALDFEHRYLG
                     FGTYFTDEAKQRLYTDDLRAATRDMDAYVEHRRHFARCSEAAPLNRLLYVDMKTFLPC
                     LNLDYTDKTAMATTLEVRVPFLNVEMVELSARMPPRLKLRGLKRKYILKRAAERVLPR
                     DVVWRKKAGFGAPVRAWLRGPLRPLVDEMLSEEAVRRRGLFRPEEVRRVVDANLSGRE
                     DFSLHVMQLLTLEMWHRTFLDAN"
     gene            complement(2659..4842)
                     /locus_tag="VF240_07575"
     CDS             complement(2659..4842)
                     /locus_tag="VF240_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEX7175126.1"
                     /translation="MKQILQNYKTGEVTVADVPPPVAQAGRVLVRAAASLISAGTERM
                     AVELGRKSLLGKARERPDLVRQVIRKAQAEGVSSTLAAVRAKLDASNALGYSAAGTVF
                     AVGEGVTEFRAGQRVACAGAGFASHAELLSVPKNLCVALPDAVDFEAAAFGTLGAIAL
                     QGVRLAEPTIGEAVVVLGLGLLGQLTVQLLKANGCRVFGIDTDPTRVALARTLGADDG
                     ALAGDAARAAVMHWSRGRGADAVLITAATSSDQPVEFAGEVSRLKGRVVAVGLVGMNV
                     PRKIYYERELTLKVSMSYGPGRYDPEYEERGHDYPFPYVRWTEGRNIEAFLDLVASGS
                     VDVRPLITHRFPIEEGERAYQLISGETSEAYLGVLLTYDTERELAGRVELRAPTKAGA
                     ASVREGAARVRVGLIGAGSYAQKFLLPNFKETGAEFVSVATATGVTAREVGRKYDFAR
                     SVSGADAVLEDGDVNLVVIATRHDSHAELARRALERGRHVFVEKPLALDDAQLDAVLS
                     AARASNASLMVGFNRRFSPHARAAHAFLAARRAPLSILYRVNAGRIPRGHWIQDAREG
                     GGRIIGEVCHFIDLIHFLTNSTTTRVYAEPVASRDHETVDDDSVFITLRLADGSNASI
                     AYLAEGDRALPKERVEIYGEGRTFVIDDFRAATAYASGRERRTRLRGQDKGQREEVRA
                     ACAVVTSGAPAPIALADLAATTRATFRILESLRTAEPRDVISDEL"
     gene            complement(5023..6138)
                     /locus_tag="VF240_07580"
     CDS             complement(5023..6138)
                     /locus_tag="VF240_07580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7175127.1"
                     /translation="MRVLIGMPDRESLGGPAACEPPFVEELRRLGVEVGEETYVYGDK
                     LSGTGLAQRVRRVVSTARRLRRRLKADGFDVVHLNTSFDTKALLRDAVTLPRLRASGA
                     RIFLKFHGSDGALLETKNALLRRMGRMLLASADGIGVLSSEERDNFVRAGADASKVFV
                     VKNVVERDFPERADERLAARLNLKEDVPRLLFIARFIPAKGLTDVLRACRIVGDGGRE
                     FALLCVGDGPARAEAEAEAARLGLAERVRFCGLMPESETGEFYAGSTALVFPTYHYEG
                     FPMTVFYAAAAALPIITTRIRAAADYLREPDNCLWVEPRNPKMLADKITRLLDEPHTR
                     AAMGQNNRVLARSFSAACVAPEYAEIYNRLLSKGQKK"
     gene            complement(6148..7266)
                     /locus_tag="VF240_07585"
     CDS             complement(6148..7266)
                     /locus_tag="VF240_07585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175128.1"
                     /translation="MRVLHILDSLNRGGAETLALDLCRNARAGGLELTFVATGGGDLE
                     DDFRRSGADFIRLRRELPVDLNLAASLREIVRERNVEVVHCHQAVEALHAYLATRGTG
                     VKRVLSFHLCSADAKNRLALKLLAPRMDANVAVSRDLLACLGREAGFDTGRNFHVVYN
                     GVDARRLTATAGAHDLRGEPGLGAEDMLFGMVGNFYADDRKDQLTVCRALPRLFDAVP
                     RSRFVFVGATPAGSPTRAYDECVDFCRRAGLEGRVRFLGKRDDIPAVLAALDVFVLSS
                     RADTFGVAAVEAMMAGVPAVLSDIGPLREVSNEGEYAALFRTGDAEDLARVLIELANH
                     AEGRTRLASRAREWATQQFSIESHIAGLIQLYGRLGGV"
     gene            7454..>7834
                     /locus_tag="VF240_07590"
     CDS             7454..>7834
                     /locus_tag="VF240_07590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7175129.1"
                     /translation="MSVVVPSYNHAPFVERTLRSVFQQTHAPRELLVIDDGSRDGSAR
                     VVERVLQDCPFPCELVARENRGLCATLNEGFARTTGRYFAYLGSDDVWLSEFLRARVA
                     ALEARPRAVLAYGGAYTIDEHDRII"
BASE COUNT         1319 a         2571 c         2672 g         1272 t
ORIGIN      
        1 gagcggcatg aagacgctct cggtgtagcc ggtgtggaaa aagtaggcgg tcgggaagat
       61 gaacatgaag aagacggcgg cgcgcgcgct cgtgtcttcg aggtcgaggc gggcgagccg
      121 gtagaacagg agaccggcgg ccacggcgcc tatgagcgag acgacgtgga aggtcaggac
      181 gtagtcgcgc aggactatgc cgaagaggcg cagcagccac gggtagagcg ggtagaagac
      241 gatccagagg cgctgatctt tgctgacgag gtcggtcgcc acgtagccgt cgcgcgcgat
      301 gtcgatgtag tgaggcacgt cccagcggtt ccaaagctcc agccagccct gaaaggagcc
      361 ggccggctgg ttcgtgagga tggcgtaggc ggccacgccg aagaccagca tgaacagctt
      421 gagcgcgacg acgaagagga cgatgcgccg gtcgaaccac ggctgatgtt ccgcggctgt
      481 cgccttgtgt gatttttcga tctgtctttg ttgtctgttc ttcgggggca tcgggaaatc
      541 accatgcaat gtgggtctgg ttcactcaag tctccgcgtc ccctccgcgt cctctgcgac
      601 tcggcggtga atctatttcc ctctcactca agttattgct ctagttcgcg tcgaggaacg
      661 tgcggtgcca catctccagc gtcaggagtt gcatgacgtg gagactgaaa tcttcgcggc
      721 ccgagaggtt cgcgtccacg acgcggcgca cctcctcggg gcggaagagg ccgcgacggc
      781 gcaccgcctc ctccgacaac atctcatcga cgagcgggcg caacggccct cgcagccacg
      841 cgcgcaccgg cgcgccgaag cccgccttct tgcgccacac cacgtcgcgc ggcagcaccc
      901 gctcggcggc gcgcttcaga atatatttcc gcttcagacc gcgcagcttc aggcgaggcg
      961 gcatccgcgc cgacagctcc accatctcga cattgaggaa cggcacgcgc acttccagag
     1021 tcgtcgccat cgccgtcttg tccgtgtaat cgaggttcag acacggcagg aaggtcttca
     1081 tgtcaacgta gaggagacgg ttgagcggcg cggcctccga gcagcgcgcg aagtgccggc
     1141 ggtgctcgac gtaggcgtcc atgtcgcggg tcgccgcgcg caggtcgtcg gtgtagagac
     1201 gctgtttcgc ctcgtcggtg aagtaggtgc cgaagccgag gtagcgatgc tcgaaatcga
     1261 gcgcggcgct gcgggcgaac ttcttggcgt tgcgcaaagg cgcggtcaaa aggccgggca
     1321 tgccgccggg cagcgtctcg gcgagcgcgc gcatgatggg gcgacgcagc gaagaaggga
     1381 tggggtcgag cgcgccggcc agcgccatcg cgaggtggcg cgggtagccg gcgaagacct
     1441 cgtcgccgcc catccccgag agcagcacgg tcaaagtctc acgcgccgcg cgcgcgacga
     1501 ggtagctgct gatgacagaa tcgatcagcg gcgattccat gtgatagacg agcttcggca
     1561 gcaacgcggc cacgtccggc tggaggatga tctcgtggta gtcggtgtcg aagagttggc
     1621 cgacgcggcg cgcccagcgc gcgtcgtcgg ggatgatgtc gtagcgcaag tcctccggct
     1681 cgaagccgat ggtgtaggtc gagacgcgcc ggccctcgga gtgtcgcttc atcatcgcca
     1741 cgatcatcga tgaatccact ccgcccgaga ggaacgaacc cagcggcacg tccgacacca
     1801 tctccagacg cacgacgcgc tcgaaggtct ccaacacctg ctcgcgccac cactccacgc
     1861 cgcgccccgt ctccaactcg tcgaacgaaa cgtcccacca ctcgcgcacg ctcacgcgcc
     1921 cgtcgcgcca cgtgaggacg tggccgggcg gaagtttctt cacgccctta aaaagagtgt
     1981 gcgggtcggg cgtccagagg aaagtgagct gctggttgag gccctcgacg ttcagcgcac
     2041 gctcgaccag cccgctcgcc agaatggcct tcgcctccga cgcgaagacg aaagacgtgc
     2101 ccgcgccgtt gtcaccctgc ggcgtcgcat agaagagcgg cttgatgccg aggcggtcgc
     2161 gcgcaaccgt caacacgcgc tctcgctcgt cccacacggc gaaggcgaac atcccgttca
     2221 gacgcggcag gcactcttcg ccccactcgg cgaacgccgc cagcagcacc tccgcgtccg
     2281 agtccgtatg aaactgccgt ccccgcccaa cgagttcggc gcgcacctcg cggaagttgt
     2341 aaatctcgcc gttgaaggtg aggacgtagc gcccgtccgc cgacagcatc ggctcgtgtc
     2401 cgccctcgct cgtgtcgatg atcgccagcc ttcggtgccc cagacacgcg cgccgcccct
     2461 cgccgtcagc cgctacggaa gtccacacac cctcgtcgtc gcgcccgcgg tgctcgatgg
     2521 ccttgagcat cgcgccgatg cgccgctccg gttcactgct gatgagtccg gcgattccgc
     2581 acatggcttc aggagtgata agtgatgagt gataagtgat gagaaagaat agggtcgtct
     2641 tcttctcttt cttatcactt ataactcatc acttatcacg tctcgtggtt ctgccgtccg
     2701 cagcgactca aggatgcgga aggtggcgcg ggtggtggcc gcgaggtctg cgagggcgat
     2761 gggcgcgggg gcgccgctcg tgactacggc gcaggcggcg cgcacctctt cgcgctggcc
     2821 cttgtcttgt ccgcgcaggc gcgtgcgccg ctcgcgcccg ctcgcgtagg ccgtggccgc
     2881 gcggaagtcg tcgatgacga acgtccggcc ctcgccgtag atttcgacgc gctccttggg
     2941 gagtgcgcgg tcgccctcgg ccaagtaggc gattgatgcg ttggagccat cggcgagacg
     3001 cagggtgata aagacggaat cgtcgtccac cgtctcgtga tcgcgcgagg cgaccggctc
     3061 ggcgtagacg cgcgtcgttg tggagttggt caggaagtgt atgaggtcga tgaagtggca
     3121 gacctcgccg atgatgcggc cgccgccctc gcgcgcgtct tgtatccagt ggccgcgcgg
     3181 gatgcggccg gcgttgacgc ggtagagaat cgagagcggc gcgcgccgcg cggctagaaa
     3241 cgcgtgggcc gcgcgggcgt ggggcgagaa gcggcggttg aagccgacca tcaacgatgc
     3301 gttggaggcg cgcgccgcgg agagtacggc gtcgagctgt gcgtcatcga gcgcgagggg
     3361 cttttcgacg aagacgtggc ggccgcgttc gagcgcgcgg cgggcgagtt cggcgtgcga
     3421 gtcgtggcgc gtggcgatga cgacgaggtt cacgtctccg tcttcaagaa ccgcgtccgc
     3481 gccggagacg gagcgcgcga agtcgtactt gcggcccact tcgcgcgccg tcacgcccgt
     3541 ggccgtggcg accgagacga actccgcgcc cgtttctttg aagttcggca gcaggaactt
     3601 ctgcgcgtag ctccccgcgc cgatgagtcc gacgcgcacg cgcgccgccc cttcgcggac
     3661 ggaagccgcg cccgccttcg tgggcgcgcg cagttcgacg cggcccgcaa gctcgcgctc
     3721 ggtgtcgtag gtgagcagca cgccgaggta agcttcgctc gtctcgccgg agatgagctg
     3781 gtacgcgcgc tcgccctcct caatcgggaa gcgatgcgtg atgagcgggc gcacgtccac
     3841 gctgcccgac gcgacgaggt cgaggaaggc ttcgatgttg cgcccctccg tccagcggac
     3901 gtaagggaag gggtaatcgt gcccgcgctc ctcgtactcc gggtcgtagc gccccggccc
     3961 gtaagacatc gagaccttca gcgtcagctc gcgctcgtaa taaattttgc gcgggacgtt
     4021 catgccgact aagccgacgg ccacgacgcg ccctttgaga cgcgacacct cgccggcgaa
     4081 ttcgacgggc tgatcggaag aggtggcggc cgtgatgagg acggcgtcgg ccccgcgtcc
     4141 gcgcgaccag tgcatgacgg cggcgcgcgc cgcgtcgccc gccagcgcgc cgtcgtccgc
     4201 gccgagcgtg cgcgcgagtg cgacgcgcgt cgggtcggtg tcgatgccga agacgcggca
     4261 gccgttcgcc ttcaggagtt gcacggtgag ctggccgagg aggcccagac ctagaacgac
     4321 gaccgcttcg ccgatggtcg gctccgcgag tcggacgcct tgcagggcga tggcgccgag
     4381 cgtgccgaag gccgccgcct cgaaatcgac cgcgtcgggc agcgccacgc agaggttctt
     4441 cggcaccgag agcagttccg cgtgcgacgc gaagcccgcg ccggcgcacg ccacgcgctg
     4501 gcccgcgcga aactccgtca cgccctcacc caccgcgaag actgtgccgg ccgcgctgta
     4561 gccgagcgcg ttcgaggcgt cgagcttggc gcgcacggcc gccagcgtgc tgctcacgcc
     4621 ttcggcctgc gccttgcgga tgacctgtcg gacgaggtcg gggcgctcgc gcgctttccc
     4681 caacagactc ttgcggccca gctccaccgc catgcgctcc gtgcccgcgc tgatgagcga
     4741 cgcggcggcg cgcacgagca ccctgcctgc ttgcgcgacg ggcggcggca cgtcggccac
     4801 cgtcacctcg cccgtcttgt agttctgcaa aatctgcttc atgcgcgaag actatcgaat
     4861 gtctgaagaa gaattacaag aagtgataag tgataagtga tgagtgatga gagagcggtc
     4921 agcaatcagc cttcagcaat cagccagaac ggccgccgct tcttgtcggg ctgaaagctg
     4981 acggctgacc gctgaaagct ccttctcatc actcatcact catcacttct tctgcccctt
     5041 cgagagcagc cgattgtata tctccgcgta ctccggcgcc acacacgcgg cggagaagct
     5101 gcgggcaagc acgcggttgt tttgccccat cgcggcgcgc gtgtgcggct cgtcgaggag
     5161 ccgggtgatt ttgtcggcga gcatcttggg gttgcgcggc tcgacccaga gacagttgtc
     5221 cggctcacgc aggtagtcgg cggcggcgcg gatgcgtgtc gtgatgatgg gcagagcggc
     5281 cgccgccgcg tagaagacgg tcatcgggaa gccctcgtag tgataggtcg ggaagacgag
     5341 cgccgtgctc ccggcgtaga actcgcccgt ctccgactcg ggcatcaggc cgcagaagcg
     5401 cacgcgctcc gcgagcccca aacgcgccgc ctctgcttcg gcctccgcgc gcgccggccc
     5461 gtcgcccacg cacaggagcg cgaactcgcg gccgccgtcg ccgacgatgc ggcaggcgcg
     5521 gagcacgtcc gtgagtccct tcgcggggat gaaccgcgcg atgaagagca gccgcggcac
     5581 gtcctccttc aaattcaggc gcgcggcgag gcgttcgtcc gcgcgctcgg ggaagtcgcg
     5641 ctcgacgacg ttcttgacga cgaacacttt cgacgcgtcc gcgccggcgc gcacgaagtt
     5701 gtcgcgctcc tccgacgaga ggacgccaat gccgtcggcg ctcgcgagca gcatgcggcc
     5761 catgcggcgc aggagcgcat ttttagtctc cagcagcgcg ccgtccgagc cgtggaactt
     5821 gaggaagatt ctggcgcccg aggcgcgcaa gcgtgggagg gtgacggcgt cgcgcaggag
     5881 cgccttcgtg tcgaaggagg tgttgaggtg aaccacgtcg aagccgtctg ctttgagacg
     5941 gcggcgcagc cgtcgggccg tcgagacgac gcggcggacg cgttgggcga gacccgtgcc
     6001 cgagagtttg tcgccgtaga cgtatgtctc ctcgccgacc tcgacgccga gccggcgcag
     6061 ctcttcgacg aacggcggct cgcaggccgc cggcccgccg agcgattccc tgtccggcat
     6121 cccgatgagc acgcgcacgg ttttctttta gactcccccg agccggccgt agagttgaat
     6181 caaccctgcg atatgcgact cgatgctgaa ctgctgcgtc gcccactcgc gcgcccgcga
     6241 cgcaagtcgc gtccgccctt cggcatgatt cgccaactcg atgaggacgc gcgcgaggtc
     6301 ttcagcatcg cccgtgcgaa agagcgccgc gtattcccct tcattcgaca cctctcgcaa
     6361 agggccgatg tcggacaaca ccgccggcac gcccgccatc atcgcctcga ccgccgccac
     6421 gccgaacgtg tccgcgcgcg aggagagtac gaacacgtcg agcgcggcga gcactgccgg
     6481 gatgtcgtcg cgcttgccga ggaagcgcac gcggccttcg aggcccgcgc ggcggcagaa
     6541 gtccacgcac tcgtcgtaag cgcgcgtcgg cgaccccgcg ggagttgcgc cgacgaagac
     6601 gaagcgcgag cgcggcacgg cgtcgaacag acgcggcagc gcgcgacaaa ctgtcaattg
     6661 atccttgcgg tcgtcggcgt aaaaattgcc gaccatgcca aagagcatgt cttcagcccc
     6721 gaggccaggc tcgccgcgca ggtcgtgcgc gccggccgtc gccgtgagcc tgcgggcgtc
     6781 cacgccgttg tagacgacgt gaaaattcct gccggtgtcg aagcccgctt cgcgcccgag
     6841 acatgcgagc aggtcgcggc tgacggcgac gttggcgtcc atgcgcggcg cgagcagctt
     6901 cagggccaga cggttcttcg cgtcggccga gcacaggtga aaactgagca cgcgcttgac
     6961 gcctgtgccg cgcgtggcga ggtaggcgtg cagcgcctcg acggcctgat ggcaatggac
     7021 gacctccacg ttgcgctcgc ggacgatctc gcgcagactc gcggccaagt tcaaatcgac
     7081 gggcagctca cggcgcaggc ggatgaagtc ggcgcccgag cgccggaagt cgtcttcgag
     7141 gtcgccgccg ccggtggcga cgaaggtcag ttcgagtccg ccggcgcgtg cgttgcggca
     7201 aaggtcgagc gccagcgttt cagcgccccc gcggttgagc gagtcgagta tgtgcagcac
     7261 tctcatcggt tcgggcgaag tgcattaata taggggcggg gcggggaaag ctcaacgcag
     7321 gagtgatgag tgataagtga tgagtgatga gttaaagaca agttcagggg cggtcgcggg
     7381 cggagctggt ggcgacgctc ctgaagaggc ggccacttat cacttatcac ttatcactca
     7441 tcactcgcct gccgtgtccg tcgtcgtccc atcctacaat cacgcgccgt tcgttgaaag
     7501 aacactgcgc tcggtcttcc agcagacgca cgcgccgcgc gagttgctgg tgatcgacga
     7561 cggctcgcgc gacggctcgg cgcgcgtcgt cgagcgcgtg cttcaggact gtccttttcc
     7621 gtgcgagctt gtcgcccgcg agaaccgtgg gctgtgcgcg acgctcaacg aaggcttcgc
     7681 gcgcaccacg gggcgctact tcgcctacct cggctcggac gacgtgtggc tgtccgaatt
     7741 tttacgcgcg cgtgtcgccg cgctcgaagc gaggccgcgc gcggtgctcg cctacggcgg
     7801 cgcctacacc atcgacgagc acgaccgcat catc
//
LOCUS       DASVES010000190         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_196022, whole genome shotgun sequence.
ACCESSION   DASVES010000190 DASVES010000000
VERSION     DASVES010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196022"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..965)
                     /gene="ccsA"
                     /locus_tag="VF240_07595"
     CDS             complement(162..965)
                     /gene="ccsA"
                     /locus_tag="VF240_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7175130.1"
                     /translation="MYAALAAYVVAAIHSVLAFINKRRAADRVTTWSLGVGFVAHTLA
                     IIADWVNDGHFPLLHLPETILFLAWTLVVVYAAAIYRYGTRAMGVFTIPLVCVLVLAS
                     NFLRGRDDLTAGHVTEGSAAWLFPIHTTLLIFAYAALFVAFTASVMYLLMERELRLKT
                     FGAVFHRLPSLATVDDVGSGAAGAGFTLLTLGLVTGVVWSSARTGRVWHNDPVEMFAL
                     LTWVLYLALLHYRTQWRGRKAAWLGVAGGALVLCTFAGAVLLGGYHAFR"
     gene            1223..1876
                     /locus_tag="VF240_07600"
     CDS             1223..1876
                     /locus_tag="VF240_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7175131.1"
                     /translation="MPTRQEAWELLCEYTKGKSLRQHALAVETAMRACAPHYGGPTED
                     ADEWGLVGLLHDFDYEMFPTEEEHPFKGANILCGRGYPDRIIRAILGHATYTGVPRDT
                     RMAQALFATDELCGFLVACALVRPTRSLDDLEVSSVKKKLKDKGFARSVNRDDIRQGV
                     EELGVGLDEHVRFVIDALRPVQHIIGLKGSDEHELGAKSQGPEETLPAQQNAPATER"
     gene            complement(1953..2267)
                     /locus_tag="VF240_07605"
     CDS             complement(1953..2267)
                     /locus_tag="VF240_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175132.1"
                     /translation="MTSSLAALVGAGASLGLAYVLPGVVERVRSTPDTAALAVLALPL
                     GIIIAASIFVYRHTARLRPLQAALAAMLSVALTLGLIIAGTFLLPALTPRPQPHANRN
                     SD"
     gene            2418..3338
                     /locus_tag="VF240_07610"
     CDS             2418..3338
                     /locus_tag="VF240_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX7175133.1"
                     /translation="MVARSTLLYMARHEGLKDFATRFAPFKKMTARFVAGETLEEAAR
                     AIRHLNSIGCTASFDHLNESVGTVEETDGEVREYLRILRAIDETGIRSNVSIKLTQFG
                     LEIDPELTYRNARTVVEDAARRGNFVRVDMEASAVTQVTFDIFRRLRSEFGLNDVGIV
                     VQSYLRRTHDDVLDLLKIPARIRLCKGAYNEPPEVAFPDKKDVDDNYVRLMQLLLSSD
                     TYHGIATHDERMIAATVEYAERQRIGKDAFEFQMLYGIRRDLQEKLAREGYNVRVYVP
                     YGKHWYPYFMRRLAERPANVWFIVKNLFKG"
     gene            3369..3887
                     /locus_tag="VF240_07615"
     CDS             3369..3887
                     /locus_tag="VF240_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX7175134.1"
                     /translation="MSDNLGWIIWTVLGVLLIIAEVFTPGFVLLWFGVGALAAAFGAM
                     LGVGLPLQFLIFLIVSITLTAASRTIFVRYFTRDDEEGAMKFGAEALPGKVGTVVTSS
                     RGAMSEGAVKVFGSIWTAYPAEGEPPLEAGDRVVVESLRGASIYVRRLDAIPDWRGQG
                     ALKDGSDDTAGG"
BASE COUNT          773 a         1226 c         1285 g          625 t
ORIGIN      
        1 tagcggtcag ccagcaaatc aacttttaaa gggcgcgtca atgcgagcgt cgtcacggct
       61 tacatacaaa tccgattcga gaatcagcat catgcgagaa ctgctataag ggcagctgac
      121 ttcaaccgct tggctgaaag ctaatagcta atagctaaca gctatctgaa cgcatgatac
      181 ccgccgagca gcacggcccc ggcgaaggtg cagaggacga gcgcgccgcc ggcgacgccg
      241 agccaggcgg ccttgcggcc gcgccactgc gtccggtagt ggagcagcgc cagatagagc
      301 acccacgtca gcagcgcgaa catctcgacg gggtcgttgt gccagacgcg gcccgtgcgc
      361 gccgaagacc agacgacgcc tgtcacgagg ccgagcgtca gaagtgtgaa gcccgcgccc
      421 gccgcgcccg agccaacgtc gtccacggtg gcgagactgg gcagccggtg gaagacggcg
      481 ccgaacgtct tgagccgcag ctcgcgttcc atcaacaggt acatcacgct cgccgtgaag
      541 gcgacgaaga gtgccgcgta ggcgaagatc aggagcgtgg tgtggatggg gaagagccac
      601 gccgcgctgc cctcggtgac gtggcccgcc gtcaggtcgt cgcgcccgcg caaaaagttc
      661 gaggccagca cgagcacgca cacgagcggg atcgtgaaga cgcccatcgc ccgcgtcccg
      721 tagcggtaga tcgccgcggc atagacgacg acgagcgtcc aggcgaggaa cagaatcgtc
      781 tcggggaggt ggagcagcgg gaagtgtccg tcgttcaccc agtcggcgat gatggcgagg
      841 gtgtgcgcga cgaagcccac gccgagcgac cacgtcgtca cgcggtcggc cgcgcggcgc
      901 ttgttgatga acgccaggac ggagtgaatc gccgcgacga cgtaggcggc cagtgctgca
      961 tacaataacg gcttcatcgg gcgaaaaaaa gaggtgcgga cggaacgcgg caattgtagt
     1021 ttatggttgt ggtgtcggca agcagggcca ctttcggcgc gcgatgttgt actgggccgg
     1081 ggccgcgtga gaaaatttgt cgtattgagt gatcttcagt tgcatgagcg cataatagat
     1141 tcaccgcaag agtcgcggag tacgcggagt tcacgcagag agttgattca aggctctgcg
     1201 tgaacaaggg aggccgaatc gtatgccgac acgtcaggaa gcctgggaac tcctttgcga
     1261 gtacacgaag gggaagagct tgcgccagca cgcgctcgcg gtcgagacgg ccatgcgcgc
     1321 ctgcgccccg cactacggcg gccccacgga ggacgcggac gagtggggcc tcgtcggact
     1381 cctccacgac ttcgattacg agatgttccc gacggaagaa gaacatccgt tcaagggcgc
     1441 gaacatcctc tgcgggcgcg gctaccccga ccgcatcatc cgcgccatcc tcggacacgc
     1501 cacctacacc ggcgtcccgc gcgacacacg catggcgcag gcgctcttcg ccaccgacga
     1561 gctgtgcggc ttcctcgtcg cctgcgcgct cgtccggccc acacgcagcc tcgacgacct
     1621 cgaagtctcc tccgtcaaga agaaactcaa ggacaaaggg ttcgcccgct ccgtcaaccg
     1681 cgacgacatc cggcagggcg tcgaagagtt gggcgtcggc ctcgacgaac acgtccgctt
     1741 cgtcatcgac gcactgcggc ccgttcagca catcatcggc ctgaaaggaa gcgacgagca
     1801 tgagctgggg gccaaaagtc aggggccgga ggaaacgcta cccgctcagc agaacgcccc
     1861 ggccactgag cgttgactcg ccgccgccga cccttgggct tgcgctccgg cgcggcgtga
     1921 aactattctg ttgaggcttt attgaaaaaa aactaatcag agtttctgtt cgcgtgaggc
     1981 tgcggccgcg gcgtcagcgc tgggagcagg aatgtgccgg cgatgatgag gcccagcgtg
     2041 agggcgaccg agagcatggc cgccagcgcc gcctgtagcg ggcgcaggcg cgccgtgtgg
     2101 cggtagacaa agatgctggc cgcgatgatt atcccgaggg gcagggcgag gacggcgagg
     2161 gcggcggtgt caggtgtgga gcgcacgcgc tcgaccacgc cggggagtac atacgccaga
     2221 cccagactcg cgcccgcccc gacgagcgcg gcgagcgacg aagtcataat tagaagcttc
     2281 caacgcatgt gggtttgaca ggttcggcgc gagtccgggc gaggtgtcct agctggcgcc
     2341 gccaagcata gcacaggccg caccgggcgg gcgcggcctc tcgccgctac gttaacttca
     2401 gacaagtcac aaaaccaatg gtcgcacgct caacactcct ctacatggcg cgccacgagg
     2461 ggctgaaaga ctttgccacc cgcttcgccc cgttcaaaaa gatgaccgcg cgcttcgtcg
     2521 cgggcgagac gctggaggag gcggcccgtg ccatccgcca cctcaactcc atcggctgca
     2581 cggcctcctt tgaccacctc aacgagtcag tcggcacggt cgaggagacc gacggcgaag
     2641 tgcgcgagta cctccgcatc ctccgggcca tcgacgagac cggcatccgc tcgaacgtct
     2701 cgatcaagct cacgcagttc ggactcgaaa tcgacccgga gctgacctat cgcaatgcgc
     2761 gcacggtcgt cgaagatgcg gcgcggcgcg gcaacttcgt ccgcgtggac atggaggcgt
     2821 ccgccgtcac gcaggtcacg ttcgacatct tccgtcgcct gcgctcggag tttggcctca
     2881 acgacgttgg catcgtcgtc cagtcgtacc tgcgccggac gcacgacgac gtgctcgatc
     2941 tgctcaaaat ccccgcgcgc atacgcctct gcaagggcgc ctacaacgag ccgcccgagg
     3001 tggccttccc tgacaagaag gacgtggacg acaactacgt ccgcctcatg caactacttc
     3061 tttccagcga cacgtaccac ggcatcgcca cccacgatga gcgtatgatc gcggctacgg
     3121 tcgagtacgc cgagagacag agaatcggca aagacgcctt cgagttccag atgctttacg
     3181 gcattcggcg cgaccttcag gaaaagctcg cgcgcgaggg ctataacgtg cgcgtctacg
     3241 tgccctacgg caagcactgg tatccctact tcatgcgccg cctcgccgag cggccggcga
     3301 acgtctggtt catcgtgaag aatttgttca aggggtaact cttcggggag gtggtgggtg
     3361 gggacgagat gagcgacaac ctcggatgga tcatctggac ggtgctcggc gtgctcctca
     3421 tcatcgccga ggtctttacg cccggcttcg tcctgctgtg gttcggagtc ggcgcgctgg
     3481 ccgccgcgtt cggtgcgatg ctgggcgtcg gcctgccact acagtttcta atcttcctga
     3541 tcgtctccat cacactcacg gccgcctcgc ggacgatctt cgtccgctac ttcacgcgcg
     3601 acgacgagga gggcgcgatg aagttcggag cggaggcgct gccgggcaag gtcggtactg
     3661 tggtcacgtc gagcaggggc gcgatgagcg agggggcggt gaaggtcttc ggctcgatat
     3721 ggacggcgta cccggccgaa ggcgagccgc ctctggaggc cggcgaccgc gttgtcgtcg
     3781 aaagcctgcg cggggcctcg atctacgtgc gccgcctcga cgccattccc gactggcgcg
     3841 gtcagggggc attgaaagac ggctcggacg atacagcggg aggttgacgc tgcgccatgc
     3901 tcggaagtt
//
LOCUS       DASVES010000191         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_13573, whole genome shotgun sequence.
ACCESSION   DASVES010000191 DASVES010000000
VERSION     DASVES010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13573"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VF240_07620"
     CDS             <1..924
                     /locus_tag="VF240_07620"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX7175135.1"
                     /translation="SLGAGVGLAFLLDYLDDTLKSVDDVDRYLHLPSLALIPATRGEN
                     RSRLKGGGHAPVEGEVTALALTTDVRSPVAEAYRHLRTSLLLSSAGQPPKTILVTSSQ
                     PSEGKTTTAVNTAIMLAQTGAQVLIVDCDLRRPRIHANFGVSNARGLTNYLSGDRDLD
                     SLVQPYDKLPNLSLLTSGPIPPNPAELLGSSEMRRLLTVLSDRFTHVVIDSPPAISFT
                     DASILSTMVDGVMLVVHGGRSSRAIVRRAKQQLVDVGANIFGVVLNNVKLDSTEYYYS
                     GYYSGYYAYEDDDESAEGAEEPAATTTGRAS"
     gene            921..1415
                     /locus_tag="VF240_07625"
     CDS             921..1415
                     /locus_tag="VF240_07625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7175136.1"
                     /translation="MSTTERGEFCVIAPDVRLGRDVRVYNFVNLYGCEIGDGTRIGAF
                     VEIQKGARVGRNCKVSSHTFICEGVEIDDEVFIGHNVNFINDKYPRATVPDGSLQTEA
                     DWTLTRTRVERGASIGTGAVILCGVRIGAGSTVGAGSVVTRDVPPGATVAGNPAREIQ
                     GGDK"
     gene            1488..2624
                     /locus_tag="VF240_07630"
     CDS             1488..2624
                     /locus_tag="VF240_07630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7175137.1"
                     /translation="MIPFVDLQAQYRAIKAEIDEAVARVLDTSAFILGREVEAFERAF
                     ADYLGARFCVGVSNGTAAVQLALSACGVGPGDEVIVPANTFFATAEAVSTAGAHPRFV
                     DCDPVSYNIDASLIEAAITERTRAIIPVHLYGQPADLDAIFRVAARHNLTVIEDAAQA
                     HGAQYRGRRVGARGRANCFSFYPGKNLGAYGEAGAVVTDDAEVARRVRLLRDHGSERK
                     YRHEIIGYNFRMEGIQGAVLGVKLPHLDGWNELRRRHAARYDELLRDSGLVLPRASSD
                     ARHVYHLYVVQTEDRDALQKHLADAGVQTGIHYPIPIHLQPAYAFLGHKPGDFPEAER
                     QAACVLSLPMFAELTDEQIERVAEAVHSFSGSGVRQAAAVEASL"
     gene            2832..>3727
                     /locus_tag="VF240_07635"
     CDS             2832..>3727
                     /locus_tag="VF240_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7175138.1"
                     /translation="MTKEATTVGIIGCGYWGPNLLRNFSENESAELRWVCDLDPKRLE
                     VMGRRYPSAETTPDCEQLFADPRLDAVVISTPVGTHYDYARRALEAGKHVLVEKPLTS
                     TARKAEELIALAERGGLTLMVDHTFIYTGAVRKIKEIVEGGELGDLLYFDSTRINLGL
                     FQSDINVVWDLAPHDLSIMDYILDRRPTSVSATGSCHIERGIENIAYVMLRFPDEFIA
                     HFHFNWLAPVKIRRTLIAGMRKMIVYDDIEPVEKVRVYDSGVTASRAGGGGDAVAAEE
                     EREAAYRTLVSYRTGDVWAPKL"
BASE COUNT          663 a         1330 c         1117 g          617 t
ORIGIN      
        1 tctctcggcg ccggcgtcgg cctcgccttc ctgctcgact acctcgacga cacgctcaag
       61 agcgtcgatg acgttgaccg ctatctgcac ctgccgtcgc tcgcgctcat cccggccacc
      121 cgcggcgaga accgctcgcg cctcaagggc ggcggacacg cgccggtcga gggcgaggtt
      181 acggcgctgg cgctcaccac ggacgtgcgc tcgcccgtcg ccgaggctta ccgccatctg
      241 cgcacttcgc tcctgctctc ctcggccgga cagccgccga agaccatcct cgtcacgtcg
      301 agccagccgt cagagggcaa gacgacgacg gccgtcaaca cggccatcat gctggcgcag
      361 accggcgcgc aggtgctcat cgtcgattgc gacctgcgcc gcccgcgcat ccacgccaac
      421 ttcggcgtct cgaacgcgcg cggcctgacc aactacctct ccggcgaccg cgacctcgac
      481 tcgctcgtcc agccctacga caagctgccc aatctcagtc tgctgacctc ggggccgatt
      541 ccgcccaacc cggccgaact cctcgggtcg agcgagatgc gccggctcct gaccgttctc
      601 agcgaccgct tcacgcacgt cgtcatcgac tcgccgccgg ccatctcctt taccgacgcc
      661 tcgatcctct cgacgatggt tgacggcgtg atgctcgtcg tccacggcgg ccgctcctca
      721 cgcgccatcg tccgccgcgc caagcagcag ctcgtcgatg tcggcgccaa catcttcggc
      781 gtcgtcctca acaacgtgaa gctcgactcg accgagtact actactcggg ctactactcg
      841 ggctactacg cctacgaaga cgacgacgag tcggccgagg gggccgaaga gccggccgcg
      901 accacgacgg ggcgcgccag ttgagcacta cggagcgggg cgagttctgc gtcatcgcgc
      961 ccgacgtgag gctcggccgc gacgtgcgcg tctacaactt cgtcaacctc tacggctgcg
     1021 agatcggcga cggcacacgc atcggcgcct tcgtcgaaat ccagaagggc gcgcgcgtgg
     1081 gccgcaactg caaggtgtcg agccacacct tcatctgcga gggcgtcgag atcgacgacg
     1141 aagtcttcat cggccacaac gtcaacttca tcaacgacaa gtacccgcgc gccaccgtcc
     1201 ccgacggctc gctccagacc gaggcggact ggacgctcac acgaacgcgc gtcgagcgcg
     1261 gcgcttccat cggcaccggc gccgtcatcc tctgcggcgt ccgcatcggc gccggctcga
     1321 cggtcggcgc cggctccgtc gtcaccagag atgtgccccc cggcgcgacc gtcgccggca
     1381 acccagcaag agaaattcaa ggaggtgata agtgatgagt gataagtgat aagaaagagc
     1441 aggcggtttt tcttatcact tatcactcat cactcatcac ttttagaatg attcctttcg
     1501 tagacctaca ggcccagtac cgggccatca aggcggagat cgacgaggcc gtcgcgcgcg
     1561 tgctcgacac gtcggccttc atcctcggcc gtgaggtgga ggcgttcgag cgcgccttcg
     1621 ccgattatct gggcgcgcgc ttctgcgtcg gcgtgagcaa cgggacggcg gccgtccagc
     1681 tcgcgctttc ggcgtgcggc gtcgggccgg gcgacgaagt aatcgtcccc gccaacacct
     1741 tcttcgccac ggccgaggcc gtctccaccg ccggcgctca tccgcgcttc gtcgattgcg
     1801 accccgtctc ttacaacatc gatgcgtcgc tgattgaagc cgcgattaca gagcgcacgc
     1861 gcgccatcat ccccgtccac ctctacggcc agccggccga cctcgacgcc atcttccgcg
     1921 tcgccgcgcg tcacaacctg acggtgatcg aagacgcggc tcaggctcac ggcgcgcagt
     1981 acagggggcg gcgcgtgggc gcgcgcggcc gcgccaactg cttcagcttc taccccggca
     2041 aaaatctcgg cgcttacggc gaagccggcg cggtcgtcac ggacgacgcg gaggtggccc
     2101 gccgcgtgcg gctcctgcgc gaccacgggt cggagcgcaa gtaccggcac gaaatcatcg
     2161 gctacaactt ccgcatggaa ggcattcagg gcgcggtgct cggtgtgaag ctcccgcacc
     2221 tcgacggctg gaacgaactg cgccgccgac acgccgcgcg ctacgacgag ctgctgcgcg
     2281 acagcggcct tgtcttgccg cgcgcgtcgt cggacgcgcg ccacgtctat cacctctacg
     2341 tcgtgcaaac cgaagaccgc gacgcgctgc aaaagcactt ggcggacgcc ggcgtccaga
     2401 ccggcatcca ctaccccatc cccatccacc tccagcccgc ctacgccttc ctcggacaca
     2461 agcccggcga tttccccgag gccgagcgtc aggccgcgtg cgtgctctcg ctcccgatgt
     2521 tcgccgagtt gacggacgaa cagatcgagc gcgtcgcgga ggcggttcac agtttcagcg
     2581 gcagcggtgt gcgacaggca gcggccgtcg aagcatcttt gtaggggcag ggcttgtccc
     2641 tattcgtact tacagaataa ttaacatgga tggacaggat ggataagata tgaagatttg
     2701 agatttcaga tttcagatgt gagatttcaa atgttctgat cctgagcatc ctgcccatcc
     2761 atgttaatta ttctttcgga aagacgggcc gcgaacttgt cctgccacac gattttgacg
     2821 acgcttgatt gatgacgaaa gaagcgacaa cggtcgggat catcggctgc ggctactggg
     2881 ggccgaacct gctgcgtaac ttctcggaga acgagtcggc ggagctgcgc tgggtgtgcg
     2941 acctcgaccc gaagcgcctc gaagtgatgg gccgccgcta cccctcggcc gagacgacgc
     3001 ccgactgtga acaactcttc gccgacccgc gcctcgacgc cgtcgtcatc tccacgcccg
     3061 tcggcaccca ctacgattac gcgcgccggg cgctcgaagc cggcaagcac gtcctcgtcg
     3121 agaagccgct gacatcgacc gcccgcaagg ccgaggagtt gatcgctctg gccgaacggg
     3181 gcggcctcac cctgatggtc gatcacacgt tcatctacac gggcgcggtg cgcaagatca
     3241 aggagatcgt cgagggcggg gaactcggcg acctgctcta cttcgactcg acgcgcatca
     3301 acctcggcct cttccagagc gacatcaacg tcgtctggga cctcgccccg cacgacctct
     3361 cgatcatgga ctacatcctc gaccgccgtc ccacctcggt ctcggccacc gggagttgcc
     3421 acatcgagcg cggcatcgaa aacatcgcct acgtcatgct ccgcttcccc gacgagttca
     3481 tcgcccactt ccacttcaac tggctggcgc ccgtcaagat tcgccgcacg ctcatcgccg
     3541 gcatgcgcaa gatgatcgtc tacgacgaca tcgagccggt cgagaaagtg cgcgtctacg
     3601 acagcggggt gacggccagc cgcgcgggcg gcggcggcga cgccgtggcc gccgaggagg
     3661 agcgcgaggc cgcctaccgc acgctcgtca gctaccgcac cggcgacgtg tgggccccca
     3721 agctcga
//
LOCUS       DASVES010000192         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_992249, whole genome shotgun sequence.
ACCESSION   DASVES010000192 DASVES010000000
VERSION     DASVES010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992249"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VF240_07640"
     CDS             complement(<1..449)
                     /locus_tag="VF240_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX7175139.1"
                     /translation="MSKFLSGRRIDPRPITGDEALADLVDETFLAYNAGRLREACQLF
                     ARKMLEPDVTVGLSLTGALTPAGLGISALIPLMRAGFVDWIITTGANLYHDTHFGIGL
                     SLHQGNTSTSDVVLREQEVVRIYDIFFDYTVLLDTDAFFRRLIEGEE"
     gene            complement(498..953)
                     /locus_tag="VF240_07645"
     CDS             complement(498..953)
                     /locus_tag="VF240_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175140.1"
                     /translation="MRVEAATNARQQPRPRLRGPVGAACLVLCAAAVAMLAAGCASRS
                     CKERLVESKESPDRRHNAAVLVRNCAGEPNGVTHVNLHAGYGTAPPADGDGSVTRGEV
                     FSIDGQRQINVVWKGAQSLQVECVGCGANQVFKKIPAWEDVQISYAERE"
     gene            complement(950..2284)
                     /locus_tag="VF240_07650"
     CDS             complement(950..2284)
                     /locus_tag="VF240_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7175141.1"
                     /translation="MQIEIAIACVLLVALTFLSLIDVAFGELSDVGLRRMIGDAEGES
                     PTAANRFLGEILENRTGFRHTLTVAILVLVVAVVVLITSVTFGWVYGASPVESVPHKQ
                     ALLIAFVVALLLTAAARQMIPRLLGSNRPEATLLRLLPLLRPFYRPLSMVAFTWRAPE
                     ARTRRGEQPAEADADADDDDDGDDLQALIDVGEEEGILEGEEGELIQSIIEFGDTRAS
                     EVMTPRMEIAALPVTATVREARDLVIDSKYSRLPVYRGQIDNVEGLIYVRDLLACWAE
                     GREDESIEPLLRPAYFVPATKPVDELLKEMQKTHAQLAMVIDEYGGVAGLVTVEDILE
                     EIVGEIEDEDTTEEEIVEIVEAEDGYYEMPGSTEVGKIERLFDFEIEDDDFTTIAGLV
                     ISEEGRVPPSGTRLTFRGLDVEVLEADERRIGRLRLRRSVGGNGEQPPEKNA"
BASE COUNT          437 a          853 c          817 g          447 t
ORIGIN      
        1 aactcctcgc cctcgatgag ccggcgaaag aaggcgtcgg tgtcgaggag caccgtgtag
       61 tcgaagaaga tgtcgtagat gcggacgact tcctgctcgc gaagcaccac gtcggaggtg
      121 gaagtgttgc cctgatggag cgacagtccg atgccgaagt gtgtgtcgtg gtagaggttc
      181 gcgccggtgg tgatgatcca atcgacgaag ccggcgcgca tgagcgggat gagcgcggag
      241 atgccgaggc ccgccggcgt gagcgcgccc gtgagcgaga ggccgacggt cacgtccggc
      301 tccagcatct tccgcgcgaa gagttggcac gcctccctga gacggccggc gttgtaagcc
      361 agaaacgtct catcgacgag atcggccagc gcctcatcgc ccgtgatcgg gcgcgggtcg
      421 atgcgccgcc ccgacaagaa tttgctcacc ttcctcttcg ccatccctcc cccttcgctc
      481 tcaactttcg tgatcactca ctcccgctcg gcatatgaaa tctgcacgtc ttcccaagcc
      541 gggattttct taaagacctg attcgccccg cagccgacgc actcgacctg caaactctgc
      601 gcgcccttcc agacgacgtt gatctgcctc tgtccgtcaa tcgagaagac ttcgccgcgc
      661 gtcaccgagc cgtcgccgtc ggccggcggg gccgtgccgt agccggcgtg caggttgacg
      721 tgcgtgacgc cgttcggctc gcccgcacag ttgcgcacga gcacggcggc gttgtgccgg
      781 cggtcgggcg actctttgct ctcgaccaga cgctccttgc aggagcgcga cgcgcaaccg
      841 gccgcgagca tcgcgacggc cgccgcgcag aggacgagac acgccgcccc cacaggcccg
      901 cgcaagcgtg gacgaggctg ctgccgggcg ttcgtcgcgg cttccaccct caagcgtttt
      961 tctccggcgg ctgctcgccg ttgccgccga cggagcgacg caggcgcagg cgcccgatcc
     1021 tgcgctcgtc cgcttcgaga acttccacgt cgagaccgcg aaacgtgagc cgggtgcccg
     1081 aaggcggcac gcgcccctcc tcgctgatga cgaggccggc gatggtcgtg aagtcgtcgt
     1141 cctcgatctc gaagtcgaac aggcgctcga tcttgccgac ctcggtcgag ccgggcatct
     1201 cgtagtagcc gtcttcggcc tcgacgatct cgacgatctc ctcctcggtc gtgtcctcgt
     1261 cctcgatctc gccgacgatc tcttcgagga tgtcctcgac ggtgacgagg ccggccacac
     1321 cgccgtactc atcgatgacc atggcgagct gcgcgtgcgt cttctgcatc tccttgagca
     1381 gttcgtccac gggcttcgtc gccgggacga aataagcggg ccggagcagc ggctcgatgg
     1441 actcgtcctc gcgcccctcg gcccagcagg cgagcaggtc gcgcacgtaa atcagaccct
     1501 cgacattgtc gatctgtccc cggtagacgg gcaggcgcga atacttcgag tcgatgacga
     1561 ggtcgcgcgc ctcacgcacg gtggccgtca cggggagcgc ggcgatctcc atacgcggcg
     1621 tcatcacctc gctcgcgcgc gtgtcgccga actcgatgat cgactgaatg agttcgccct
     1681 cctcgccttc gaggatgccc tcctcttcgc ccacgtcgat gagcgcctgt aggtcgtcgc
     1741 cgtcatcgtc atcgtcggcg tcggcgtccg cctcggcggg ttgttcgccg cgccgcgtgc
     1801 gcgcctcggg cgcgcgccac gtaaaggcga ccattgatag cgggcgatag aaggggcgca
     1861 ggagcggcag cagccggagc agcgtcgcct ccggcctgtt cgaccccaga aggcgcggga
     1921 tcatctggcg cgcggcggcc gtcagcagga gggcgacgac gaacgcgatg aggagcgcct
     1981 gcttgtgcgg cacgctctca actggagacg cgccgtaaac ccagccgaat gtgacggagg
     2041 tgatgaggac gacgactgcg acgaccagaa cgaggatggc gaccgtcagc gtgtgccgaa
     2101 agcccgtgcg gttctccaga atctcgccga ggaaacggtt ggcggccgtc ggcgactccc
     2161 cctcggcgtc gccaatcatg cgccgcagcc ccacgtcgct caactccccg aaggccacat
     2221 cgatcagcga caggaacgtc agcgcgacga gcagcacaca cgcgatggct atctcgattt
     2281 gcataaagaa gctgttagct attagctgtt agctttcagc tttcggcaga agccgtccgg
     2341 ggcgtcgtcg ccaacagtga tgtaattcgc ccttccggct gacagctaac cgctaatagc
     2401 taacagcttg ataattatat tccaagacgg cggcgaagtc ggagttcgag cgcgttcatc
     2461 tggccccggt cggtctcgtg gtcgtagccg caaaggtgga gcaagccgtg gaggatgagt
     2521 tgcgccacct cgccgtcgaa actcaggccg tgct
//
LOCUS       DASVES010000193         6893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_529102, whole genome shotgun sequence.
ACCESSION   DASVES010000193 DASVES010000000
VERSION     DASVES010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529102"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VF240_07655"
     CDS             <1..625
                     /locus_tag="VF240_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175142.1"
                     /translation="QRMQIIAVRQRDTRMVGCTGDAQIIISNSDLEDLIVSVLEACDS
                     PADVRTLRSLVMSRLPVMDIYLVPLGGDDAEDGHRGFDPVDGRENPEQTLLRREAEGE
                     VGSFVDRFLQGLHEAVRGKTKQYDRIIGVLWHCYLSPVHITQLEAAARLNVSDSLVSD
                     YRRRIEQQLRALAFSEIEEARRFETALRERVSMLISVEVNVNAGASA"
     gene            944..2065
                     /gene="wecB"
                     /locus_tag="VF240_07660"
     CDS             944..2065
                     /gene="wecB"
                     /locus_tag="VF240_07660"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7175143.1"
                     /translation="MSRTLKVINVVGARPNFMKVAPIVEAMGRRATEFAPLVVHTGQH
                     YDERMSDAFFRDLGMPRPDVHLGVGSGSHARQTAAVMERFEPVVLEEQPDWVLVVGDV
                     NSTLACALVCAKLLVRVAHVEAGLRSRDRTMPEEINRLLTDQISDLLLTPSADADRNL
                     LAEGIPAGRIRMVGNVMIDSLFKQLERARSSTAREDLGVGGRDYAVVTLHRPSNVDDA
                     TTLAGIISALEELGARLPIVFPVHPRTRQNLAEFGLLDAVERRGRLLLVEPLGYLDFL
                     RLYSGARLVLTDSGGIQEETTALGIPCLTLRENTERPVTVELGTNRVVGADPGRIVAA
                     AEDALAKDRQNEPPRVPPLWDGKTSDRILDALTESGGKA"
     gene            2461..3996
                     /locus_tag="VF240_07665"
     CDS             2461..3996
                     /locus_tag="VF240_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX7175144.1"
                     /translation="MSTGIQKESVSLTVRATWMMLAKTLAFIFSFALPLLLVRRLDQA
                     EFGLYKQVFLIVGSAVLIFPFGFHMSAYYYLPREQERKGQIVFNILLFYVLIIGAVCL
                     AILLYPSMLEAILGSRELTEYAPLISLIVFTWGCSSFLETVAVANQEIRLATIFIVTA
                     NSTKTLFLVGAVVIFGSFRMLLYAAILQGVLQLVVLLSYLRVRFGRFWREFDWQLLCA
                     QLSYALPLGVSATILRFQIDLDSFFVSKQLGAAALAVYAIGCLNIPLFFLISDAVGSV
                     MIPRVSYLEKTGAKREIVELLARMVRKLSAIALPVYALLLVVGPEFITVLFTTQYSAS
                     WPIFAINTAQIPLSVITSAYDPVLRAYPEQRYFLLRVRMFLLALLAVMLWQGTRRYGM
                     MGAISMVVLISAAERVIISIRVGRLLGLRSRDWLLLKDVAKMLVVVALASCVAVLVRL
                     SMGQLPALAVLCVCGAVFSFCYALGLLLLGVITPEERTFIYQSAALMRRRIFGAPRQD
                     FVA"
     gene            4220..6088
                     /locus_tag="VF240_07670"
     CDS             4220..6088
                     /locus_tag="VF240_07670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7175145.1"
                     /translation="MGISIEAQNPTEFEPAAAAVTAVEGCDRRRYKTQQITELLEIEE
                     SRRTGLLAAWEQLLASDPVGTLFQGPVWCLEWYRAYHERYQPCVLAVTYGDTLVGLVP
                     LAVERATGELVFASGIMSDYRDVLALPQHKQTVIAGLLQFYCAGGFSQPLRLGPTLPE
                     SNTATLVFAASREVPGAYAIPRSHPGWRWWPGGDTQSDFLKKKTIRQAIKHYERQGPV
                     RLDRINTVETWHEFKHEFFDQHSLRQLYAGRRPSFNSGMKQSFYDSLMLNSPGAVHVI
                     ALRVADRIIAAHYGPVHDKVLYWGAPAFDVREAQRSPGLVLLALIIQDAERLGLRGID
                     LTIGVEEYKRRFSSSSVTLPTVEIHCGRRRYYAQGLRDRVVGGAKLLLEKTKRPDAWE
                     QAKEHLDRVGERAQRVRELGIKESCIRVARRAVAAVGERARWLTLTVTPAALQPVGPV
                     LAAGEDCSFHTDELRDMLKWDGPSAETARAIVVVVRRAPVDLRKGRTLHTLLVAGRLA
                     CWGWSYRPQGPERIDVTQTVLEYAPNSVSLYDFHTVPEFRGRRLYQALLTHILHTRFA
                     EGAELAYIMVRDTNVASRKAIERVGFRVVAVDEISRLLGRSRIRHAPVGAADGPSA"
     gene            6218..>6893
                     /locus_tag="VF240_07675"
     CDS             6218..>6893
                     /locus_tag="VF240_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175146.1"
                     /translation="MRTIVPAKRSGTALNETWGAGRGHLVSYIGLLLFTAFVFFRPYE
                     LFSIPALNEGAEWLAIFTLAVFLPSQFAAEGNITARPREVNLALLLLAAALLSVPMAI
                     SPGEAWDNFVEFFKVICIFVVIVNVVRTERRFRGMLILAMAVSVLVSFNAFSDYRSGL
                     LAMRGERVLGVLGGLFANPNDLALHLVTMVPLAAGLALARRGVLKKICYGVCAALFVA
                     GIVVSFS"
BASE COUNT         1219 a         2108 c         2172 g         1394 t
ORIGIN      
        1 acagcgcatg cagatcatcg ccgtgcgcca gcgcgacacg cgcatggtcg gctgcacggg
       61 cgacgcgcag atcatcatca gcaactccga cctcgaagac ttgatcgtct ccgtcctcga
      121 agcctgcgac tcgccggccg atgtgcgcac gcttcgtagc ctcgtcatgt cgcgcctgcc
      181 cgtgatggac atctatctcg tcccgctggg cggcgacgac gcggaggacg gccaccgcgg
      241 cttcgacccc gtggacgggc gcgagaaccc cgagcagacg cttttgcggc gcgaggccga
      301 gggcgaggtc ggctccttcg ttgaccgctt cctgcaaggt ctgcacgagg ccgtgcgggg
      361 caagaccaag caatacgacc gcatcatcgg tgtgctctgg cactgctact taagccccgt
      421 gcacatcacg caactggagg ctgccgcccg cctgaacgtc tccgactcgc tggtctccga
      481 ctatcgccgc cgcatcgagc agcagttgcg cgcgctcgcc ttctccgaga tcgaagaggc
      541 ccggcggttc gagacggcgc tgcgcgagcg cgtcagcatg ctcatcagcg tggaggtcaa
      601 cgtcaacgcc ggcgccagcg cctgacgcgg gcgtggccgc acgtagcgaa agcgaggcgc
      661 gggggggttg aagcctgggc aaggtttccg ccggacggcg gagggggccg caaggccttt
      721 ttttctggtc acgagacgcg cagcccttag cgtcgccgac caaagccaag ccgattacaa
      781 gcgcctccgc gaactgtcga aatctatccc gacgcgtgca aacgtcacca tgcgagagcc
      841 gcgccgggat tgttagactg taacgggcag gactcatcag ccgtcaatgg gcgaggccgt
      901 gactgtaacg ctgggctgcc acccacaccc ttaactgatc gccatgagcc gaacgctcaa
      961 agttatcaac gtcgtgggtg cgcgtcccaa cttcatgaag gtcgcgccca tcgtggaggc
     1021 catggggcgc cgcgccacgg agttcgcgcc actcgtcgtg cacaccgggc agcactacga
     1081 cgagcgcatg tcggacgcat tcttccgcga cctcgggatg cccaggccgg acgtgcacct
     1141 cggcgtcggc tccggctcgc acgcgcggca gaccgcggcc gtgatggagc gcttcgagcc
     1201 ggtcgtgttg gaagagcagc ccgactgggt cctggtcgtc ggcgacgtga actcgacgct
     1261 cgcctgcgcg ctcgtctgcg caaaactgct cgtccgcgtg gcacacgtcg aggccggcct
     1321 gcgcagccgc gaccgcacga tgcccgagga gatcaaccgc ctgctcaccg accagatcag
     1381 cgacctgctg ctcacgccct ccgcggacgc cgaccgcaac ctcctcgccg agggcatccc
     1441 cgcggggcgc atccgcatgg tcggcaacgt gatgatcgac tcgctcttca agcagctcga
     1501 gcgagcgcgc tcgtccacgg cccgcgaaga cttaggcgtt gggggccgtg actacgccgt
     1561 cgtcacgctg caccggccct cgaacgttga cgacgcgacg acgctcgccg gcatcatctc
     1621 ggcactcgaa gagctaggcg cgcgtctgcc cattgtcttc cccgtccacc cgcgcacgcg
     1681 tcagaacctc gccgagttcg gcctgcttga cgcggtcgaa cggcgcggcc gcctgctcct
     1741 cgtcgagccg ctcggctacc tggacttcct gcgtctctac agcggagcac ggctcgtact
     1801 caccgactcg ggcggcatcc aggaggagac gacggcgctc ggcatcccct gcctgacgct
     1861 gcgggagaac acggagcgtc ccgtcaccgt cgagctgggc acgaaccgcg tcgtcggcgc
     1921 cgaccccggc cgcatcgtcg cggcggcgga ggatgcgctc gcgaaagata ggcagaatga
     1981 gccgccgcgt gtgcccccgc tctgggacgg taaaacctct gaccgcatac tcgatgcctt
     2041 aacagaatct ggcgggaaag cgtgagatac atgcatctcg ccctggacgg agaacccagg
     2101 gatttgtgga ttattatgat ctgttctttg tccgacgtaa cttccgaggc gtgacaagcg
     2161 gcgtccgttt agaggttcca gagggtattg cgcgcgaggc gctttataca ggtaaccgcc
     2221 ggtttcatgt tagagcgggt gggccagcat agccgccttc cggctatccc gcggtatttt
     2281 gtaatagcta gcctggctga gctgaacgct ttccgtattt gtctgcggtt atacccgcgg
     2341 agagcgggtg tgcgagccgt ccagttgccg ccggctagca tcgactccga tgagaatcgc
     2401 aattaaacta gcgctactcc ccgacgaagc cctcggcgtt aaacaaatag gtgggctggc
     2461 atgagcaccg gcatccagaa agaaagtgtc tccctcacag tgcgggcgac ctggatgatg
     2521 ttagccaaga cgcttgcttt catcttcagc tttgccctgc ctctgctgct ggttcgtcgc
     2581 ttggatcagg ctgaattcgg gttatacaag caggtctttc tgattgttgg gagcgctgtt
     2641 ttaatcttcc ccttcggctt ccacatgagt gcttactatt acttgccgcg ggagcaagag
     2701 cgtaaaggtc agattgtctt taacatccta ctcttctacg tactcatcat cggtgctgta
     2761 tgtctggcaa tcctactata tccgtctatg cttgaggcca tccttggcag tcgggagttg
     2821 accgaatacg cgccgctgat ctcactgatc gtgtttacgt ggggctgttc atcattttta
     2881 gaaacagtcg cggtagccaa tcaggagatc agactggcga cgatattcat cgtcactgct
     2941 aacagcacta agacactctt cctggtgggc gccgtcgtca tctttgggtc gttcaggatg
     3001 ctgctctatg cggcgatctt acaaggtgtg ttgcagttag tcgtgctcct gagttattta
     3061 cgcgtgcgct tcggccgctt ctggcgcgag ttcgactggc agctcttatg tgcgcagttg
     3121 tcctacgcgc tgccgctagg cgtttccgcc acgatcctgc gcttccagat tgacctggat
     3181 agcttcttcg tttccaaaca actgggcgcg gcggcgctcg ccgtctatgc tatcgggtgt
     3241 ctgaacatcc ctctgttctt tctgatcagc gacgccgtcg gatcagttat gatcccgcgt
     3301 gtgagttatc tggaaaagac tggggcgaag cgtgagatcg tggagttgtt agcccggatg
     3361 gtgcgcaagt tgtcggccat cgcgctcccg gtgtacgccc tgttgctggt ggtcgggcct
     3421 gaattcatta ccgtcctgtt caccacgcag tacagcgcca gttggccgat cttcgccatc
     3481 aacacggcac agatcccgct gagtgtaata actagtgctt atgatccggt gctgcgcgcc
     3541 taccccgagc agcgatattt cctgctgcgg gtgcggatgt ttctgctggc attgctcgca
     3601 gttatgcttt ggcaaggcac aagacgctac ggcatgatgg gtgcgatctc tatggtagtc
     3661 ctaatcagcg ccgccgagcg ggttataatt tcgatcaggg tcgggcgtct gctcgggttg
     3721 aggagccgtg attggttgtt actgaaggac gtggccaaga tgctggtcgt ggtcgcactg
     3781 gcaagctgcg tagcggtgct cgtccgcctg tcgatggggc aactgcctgc gctcgccgtg
     3841 ctgtgcgttt gcggcgcggt attctcgttc tgctacgcgc tcgggctgct actgttaggc
     3901 gtcattacgc cggaagagcg cacgtttata taccagagtg ccgctctgat gcgccgccga
     3961 atcttcggcg cgccgaggca ggatttcgta gcatgaccgc ggcgcgcccc cgcagtaagg
     4021 gcgtgcgcga gatgcaaatt ctaacctttt gacgctttca atcagcatcg ttaaaggggg
     4081 cgcctgaaag ctacacaggg acgaaatggt acaagcgagc ttaaagtgtt gatattctaa
     4141 ttgagtctac ttgcgccgcc ggcacgcgct gtgtgctaat gagtgcggtt ggtttgtgta
     4201 agttgttgag aataagttga tgggaataag tatcgaagcc cagaacccga cggagtttga
     4261 gccggcagcg gccgccgtaa ccgcagtcga gggctgcgac cggcggcgct ataagacgca
     4321 gcagataacc gaactgctgg agatcgaaga gtcgcggcgg acgggccttt tggcggcatg
     4381 ggagcagctg ttggcgtctg atcccgtggg gacgctgttt caagggccgg tctggtgtct
     4441 ggagtggtat cgcgcctacc acgagcgata ccagccttgc gtgctggcgg tgacgtatgg
     4501 cgacaccctc gtagggctgg tgccactggc cgtcgagcgg gcaacgggcg aactcgtgtt
     4561 cgccagcggc atcatgtccg actaccgtga cgtgctcgcc ctgccacagc ataaacaaac
     4621 cgttatcgcc ggcctgctac agttttactg tgcgggcggc ttcagccagc cattgcgact
     4681 ggggccgacg ttgccggaat cgaacacggc gactcttgtg ttcgccgcca gcagggaagt
     4741 gcccggcgct tacgctattc cccgctcgca tccgggctgg cgctggtggc cgggcggcga
     4801 cacgcagtca gactttttaa agaaaaagac gatccgtcag gccattaaac actatgagcg
     4861 gcaggggccg gtgcggctcg accgcatcaa tacggtagag acctggcacg aattcaagca
     4921 tgaattcttc gaccaacact cgctccgcca gctctacgcc ggccgccgcc cctcgttcaa
     4981 cagcggcatg aagcagtcct tttatgactc gctcatgcta aacagtcccg gcgccgtaca
     5041 cgtcatcgct cttcgcgttg cggaccgcat catcgcagcg cactacgggc ctgtgcatga
     5101 caaagtgctc tactggggcg cgccagcctt cgacgtccgc gaggcgcagc gctcgcccgg
     5161 gctggtgctg ctggcgctga tcattcagga cgcggagcga ttgggcctgc gcggtatcga
     5221 cttaacgatt ggcgtcgaag agtataaaag aagattttcc agtagcagcg tgacactgcc
     5281 gaccgtggaa atccactgcg gccgccggcg ctattacgcc caaggcctac gtgaccgagt
     5341 cgtcggcggg gctaagctgc tgctggagaa aacaaaaagg ccggacgcat gggagcaggc
     5401 caaggagcac ttagaccgtg tgggtgagcg agcgcaaagg gtccgcgaac tcggaataaa
     5461 agagagctgc atacgcgtgg cccggcgcgc cgtcgccgcg gtcggtgagc gcgcccggtg
     5521 gctcacgctg accgtcaccc cggccgcctt gcaaccggta gggcccgtcc tggctgccgg
     5581 agaggactgc tcctttcaca cggacgaact gcgcgacatg ttgaagtggg atggcccctc
     5641 ggcggagacg gcgcgcgcga ttgtagtggt ggtgcggcgc gcgccggtgg acctgcgaaa
     5701 gggacggacg cttcacaccc tgctggtggc cggtcgtctc gcatgctggg gttggtctta
     5761 ccggccgcag ggtccggagc ggatcgacgt gacccagacg gtactcgaat acgccccgaa
     5821 ctctgtctcg ctttacgact tccacaccgt ccctgaattt cgcgggcggc ggctctatca
     5881 ggcacttctg acgcacatcc tgcacacacg cttcgccgaa ggggctgagc tggcctacat
     5941 catggtgaga gatacaaacg tggcgtcgcg taaggcaatc gagcgggtag ggttccgcgt
     6001 ggtcgcggtg gatgagataa gccggctact gggccgcagt cgtatcaggc acgcgccggt
     6061 gggcgcggcg gatggcccct cggcgtaagg ggactaattg agcgacgacc gcctactgac
     6121 agattacaag accgtgaggc ccccgggttg gcacgatcag cacgagggtg aacggcccgc
     6181 gccagacgcc gacgaagagt tagcccgtga ggggcatgtg cgtacaatcg tgcccgccaa
     6241 gcgttcgggc accgcgctga acgaaacctg gggcgcgggg cggggccacc ttgtcagtta
     6301 tatcggcctc ctcctcttca cggccttcgt cttcttccgc ccgtatgagc tcttctcaat
     6361 tcccgccctg aacgaggggg cggagtggtt ggcgattttc acgctcgccg tcttcctccc
     6421 gagccagttc gccgccgagg gtaatatcac cgcgcggccg cgtgaagtta acctcgccct
     6481 tctcctgctg gcggcggcgc tgctctctgt ccccatggct atcagccccg gcgaagcctg
     6541 ggacaacttt gtcgagttct ttaaagtcat ctgcatattc gtcgtcatcg ttaacgtggt
     6601 gcgcacggag cggcgcttcc gcgggatgct cattctggcg atggcggtga gcgtgctggt
     6661 gagtttcaac gcctttagtg actaccggag cgggctttta gcaatgcgtg gtgaacgcgt
     6721 gctgggcgtg ctgggcggcc tcttcgccaa tccgaatgac ttggccctcc acctcgtcac
     6781 gatggtgccg ctcgcggcgg gactcgcgct cgcccggcgg ggggttctga agaaaatatg
     6841 ttacggcgtg tgcgcggcgc tcttcgtcgc gggcatcgtc gtctccttct cgc
//
LOCUS       DASVES010000194        14149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1053238, whole genome shotgun sequence.
ACCESSION   DASVES010000194 DASVES010000000
VERSION     DASVES010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053238"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VF240_07680"
     CDS             <1..1083
                     /locus_tag="VF240_07680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX7175147.1"
                     /translation="DASGAAGGSVVSVRDERGRFVAQALYSDRSEIALRLLTASDEAI
                     DREWWRRRLREAISRRAGLDAEADAFRLVYSEGDLMPSLIVDKYSDVLVMQTLSQGTE
                     ALKPMLVELLVEELSPRAVVERNDVRVRALEGLELESGVLYGDAPDLMEIVQHGVRFR
                     VSPLGGQKTGAFLDQRENHLAARTHARGRALDCFTFDGGFALNLASACESVTGLDVSA
                     EATAAARRNAELNGAANVEFREANVFDALREMESSGARFDTVVLDPPAFAKNRASIPA
                     AARGYKEINLRALKLLARGGVLVTCTCSYHMSEGMFLEVIAEAAADAHRRLQIVERRT
                     QSADHPVLVGVPETHYLKCIIARVIE"
     gene            complement(1169..2653)
                     /locus_tag="VF240_07685"
     CDS             complement(1169..2653)
                     /locus_tag="VF240_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7175148.1"
                     /translation="MFSLTTLALLLASLFTWPAPASRVVMQDAAQAARPSVTLADCDV
                     PDVKGKARCGIYEVFENRVTRQGRKIGLKIVVLPATGTERAPDAFFYLEGGPGVSAID
                     SASYIAQEFASLRERRDLVFVDQRGTGGSNPLVCPMYDPADPQSALREFFPPESVRRC
                     RTALEAKADLTLYTTPVAADDLDEVRAALGYERINLFGASYGTRAALVYLKRHPRSVR
                     SLLLQGVAPTHRFMPLDFPVSAQRALDGVIGECERDEACRAAFPRVRAEAREVFEKLK
                     KGPVEVEIKLRQTGATARVKLSRNLAAEAVRYMLYNSGASPRVPLFIHRAAGGDFKPL
                     AESALNYRRNLVDADSNGLYLSITCAEDLPFIKPGEGERTEHTFIGHYRLTEQRAACA
                     LWPRSPVPADYSQPTRSDAPVLILTGQWDPVTPPSQGDEVAKSLSNSTHLVVPQGGHG
                     FGGLQNRDCIRRLMIEFVERGTAKGLDASCVSGIKRQAFATTMP"
     gene            complement(2707..4170)
                     /locus_tag="VF240_07690"
     CDS             complement(2707..4170)
                     /locus_tag="VF240_07690"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7175149.1"
                     /translation="MTDTSRAHGLSTAALRRTSAALLALALLAAGAVPVRAQAVQLTP
                     HQQLLRDIYKELVEINTTDTPAGDNTRAARAMAARLRAAGFAEGDVRVVVPTANERKG
                     NLVARLRGTGAKKPLLLLAHIDVVEALKEDWSDGLDPFKLTEREGYFYGRGTGDDKAM
                     AAVFIANLIRYKQENFKPERDLIVALTADEEGGDFNGVEFLLKEHRALVDAEFGINEG
                     AGGRSRQGTPLFNGVQASEKVFQSFTLEVKNKGGHSSLPDRDNAIYRLAAGLDRLSKF
                     DFPVNLGEVTRAYFARMSKLEKGPAVADMAAVANERPDPSAAERLASKSPYYNALMRT
                     TCVPTMLTGGHAENALPQTARATVNCRILPQESAADVERTLTRILADDQIKVAAIAQS
                     KPSSPSPLRAEVMQPIESLTAEMWPGVPVIPVMGTGATDSLYFRQAGVPMYGVSGLFS
                     DIDDNRAHGRDERVGVKELYDSQEFLYRLVKALSSAK"
     gene            complement(4167..4628)
                     /locus_tag="VF240_07695"
     CDS             complement(4167..4628)
                     /locus_tag="VF240_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7175150.1"
                     /translation="MNKWKHLFVDGEFAARERILSGLTLEQVTRRLSPQSHTIYEELW
                     HTTRWQNIVVNRDEEDEAWQRGEVYPPDAPASEREWSALVEEFLAGLDRALEWADSPE
                     KLAAEIDPDFTMADALRSLAVHNAHHLGKILALRQALGAWSPGEQKEPTTV"
     gene            complement(4688..5227)
                     /locus_tag="VF240_07700"
     CDS             complement(4688..5227)
                     /locus_tag="VF240_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175151.1"
                     /translation="MRHTHTSSVTLLVLLATVLLYAAAAATIAPPSAIAQKRGRAVVC
                     GDPTVRCRTGQIEFPPYNIPFQIPARAIIWETEPFYAIILRSMRVKDEFDECERFIPE
                     EDRLEAQAIFPRNKVFASRCMEPGDLYYTNTDPKQRFMAVYAGRTRAAAAAMLEKVKA
                     TGKYPGANLRQMRAGFNGT"
     gene            complement(5249..6958)
                     /locus_tag="VF240_07705"
     CDS             complement(5249..6958)
                     /locus_tag="VF240_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7175152.1"
                     /translation="MSSRTFWRIAAVFVVALGSLLGGIIGGFASNPLLAGRNATGPAA
                     AVEGSYTEALGVVAGEYAEEIDYEKASHAAIQGMLLALDPHSNYFAPAEYTRLMQDQE
                     ARYVGIGVSILRHRDGVYVQSPLPDTPAARAGLRFGDLIAEVDGQDAREWTTAQTAKA
                     VRGEPGKPVSLKIMRAGSEVPEHFQIVRASVPQSSVRDAYIVRPGTGYVALGSFNHKT
                     TDELREALERLRTEGMRQLVLDLRNNPGGLLDQSISVASLFIERGKTIVSVRGREGER
                     ERVYRSVGTDPVDYPLVVLINRNSASAAEIVAGAVQDHGRGLVVGETSFGKGLVQKVY
                     PLPYGAGLTLTTAKYYTPYGRLIQRNYTSGSYYDYVTRRDEEDEQQSALPLSPAQAAP
                     PGPEAAPAPQPTPTGTAVTAAGGRVFYGGGGIAPDHGIKPRNVNTPLRGRVFDATFHF
                     ARLLAAGQAPGFENFRAPAEPQFGRFPRPTDFTINDRVVEAFREFVRRDPAHALTTAQ
                     VDAEVEYVRLRLREDLVTASFGGDAGARVILEGDPQLLRALELFTEAKQLAENIGRAA
                     ATN"
     gene            7224..8087
                     /locus_tag="VF240_07710"
     CDS             7224..8087
                     /locus_tag="VF240_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX7175153.1"
                     /translation="MSSLLLSVVIPAYNEAPRLGRTLDQVLAYLNEQQYESELIVVDD
                     GSRDETVAVAEGRFEGAARGRVATRVIRVEPNRGKGNAVRTGLLAARGRVALFSDADL
                     STPITEAPKLTGPIRSGQYDIVFGSRALDRSLIGVHQPWQREQSGRVFNLLMRLVTGL
                     PFWDTQCGFKAFRMDVCRAVVEGAVIDRFGFDVELLYIAHRSGVRLLERAVRWDDAAG
                     SKVSFRSGLQGFDELRQLRRNSRRGVYDEAMARARERAERVRDAGPLLLPGEAPPETD
                     ASPELAAARRI"
     gene            8270..9028
                     /locus_tag="VF240_07715"
     CDS             8270..9028
                     /locus_tag="VF240_07715"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX7175154.1"
                     /translation="MVARLNVNIDHVATVRQARRAPEPSLIAAAALCESAGADGITVH
                     LRGDRRHIQDEDLRTLRRTLTTYLNAEMAATDEMLKIALDVRPDCVTLVAERADELTT
                     EGGMDVRAGVETVRAAVGVLREGGILTSLFIDPEAAQIEAARASGAEQVELCTAAYAA
                     ATLGGLAVHTGGRAAEELQRLREAARLAAQHGLQVAAGHGLTYRNVGAVAAIREIVEF
                     NIGHNVVARAVLVGMDGAVREMRERIREGAMGTQ"
     gene            9294..10667
                     /locus_tag="VF240_07720"
     CDS             9294..10667
                     /locus_tag="VF240_07720"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX7175155.1"
                     /translation="MTSRVAGAAGPRFLFATAFACLALCLAAPDAFAQQPSASPTPAA
                     TPDVPALAKIEFIGLSRVKQEEATEATGLQAGQPIDVDLVDAAAARLLDSGLFQKLSY
                     SYRAAKGQATVVFTVEEMGARAPVVFDNFVWFSDREMDEYLRKSISGYDGTAADAGTM
                     TEKIAKALGEMLLLKGIKGRVEYRPSADPSGRNPTHLFAVRDATGLRVCSISFPGAAA
                     VREQVLVQNSGRLTSEEFSQQSALAFAETNLLPLYRERGNLRAAFRAPQARPATEDDC
                     LGVAVSFPVDEGIAYVWEKAEWWGLESLPAAALDAALGMRPRELVNLSKIEKGLEAAR
                     KLYARKGHLAPKLQPTFVFDDAARSVTYRVVVVEGPQYRMGELFITGLSEKDTNNVRT
                     RWSLPPREPYDAEYLDEFLKKAVAEFLRDPSTDARALGKLKVDTNVRPDKEKLIVDVS
                     IDFKQQK"
     gene            complement(10930..11985)
                     /gene="ruvB"
                     /locus_tag="VF240_07725"
     CDS             complement(10930..11985)
                     /gene="ruvB"
                     /locus_tag="VF240_07725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7175156.1"
                     /translation="MSQAISEAPMADAPREIAGAATPEDQAVELSLRPRSLDEYIGQP
                     KITTNLKIFLRAAKARRECLDHALFFGPPGLGKTTLSQIIAHEMDAPLKCVSAPAVER
                     GGDLAAVLTNLQEGEVLFIDEIHRLKPQIEEVLYPAMEDYQLDLMIGQGAAARSIKLD
                     LPKFTLVGATTRAGLITAPLRGRFGIVFHLDFYSRKDLEIIVHRSAEILNVEIEASGA
                     GEIARRARGTPRIANRLLRRVRDYAEVLADGRITQALARRALDEMEVDRFGLDEIDRK
                     VLTTIIEKFDGGPVGVGTISASISEERESIEEIIEPYLIQIGFLNRTPRGRMVTRAAY
                     KHLGFPVPKSERGAMAS"
     gene            complement(12127..12534)
                     /locus_tag="VF240_07730"
     CDS             complement(12127..12534)
                     /locus_tag="VF240_07730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX7175157.1"
                     /translation="MPLQLDVITPERLLVSESVDSVNLPGVGGELGILPGHTPLISQL
                     QTGVLSYAQSGTTRRLLVSGGFVEVNADRVSVLADMAEFPEEVDTQNARREREEAERR
                     LGAFAGTPEEMAALRTDLERATARLQLAADNPR"
     gene            complement(12632..>14149)
                     /gene="atpD"
                     /locus_tag="VF240_07735"
     CDS             complement(12632..>14149)
                     /gene="atpD"
                     /locus_tag="VF240_07735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7175158.1"
                     /translation="NTAGSSGQTGRVVQIIGPVMDVEFTDGYLPPIYQALRITSEGFD
                     VPTPIDIIAEVEQHLGEGRVRTVSMQPTEGLVRGMTAIDTGGPITMPVGESTLGRILN
                     VIGQPVDNLGPVHSEQRYPIHRHAPLLAEQSTELQMFETGIKVVDLVQPFLRGGKIGL
                     FGGAGVGKTVLIMELINNVAKAHSGFSVFAGVGERTREGNDLLREMVESKVINFGDSF
                     YESYEETGVFDMTKVDMRSVPESKVALVYGQMTEPPGARLRVALSGLTVAEYFRDQGL
                     DVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPNLATEMGELQERITSTRTGAITSI
                     QAVYVPADDYTDPAPATTFAHLDAVTALSRQIVELGIYPAVDPLTSSSRILDPRIVGE
                     EHYNTAQRVKQILQRYKDLQDIIAILGLDELSEEDRLVVSRARKIQKFLSQPFFVAEQ
                     FTGLKGAFVKTADTIRGFQEIADGKHDDLPEQAFYMVGTIEEARQKAERMAAELG"
BASE COUNT         2281 a         4484 c         4952 g         2432 t
ORIGIN      
        1 gacgcttcgg gcgcggccgg cggctcggtg gtcagcgtgc gcgacgagcg cggtcggttc
       61 gtcgcgcagg cgctctacag cgaccgctcc gagatcgcgc tccggctcct caccgcttcc
      121 gacgaggcga ttgaccgcga gtggtggcgg cgccgcctgc gcgaagccat cagtcgccgc
      181 gcgggcctcg acgccgaagc ggacgccttc cggctcgtct actcggaagg cgacctcatg
      241 ccgtcgctca tcgtggacaa atattcggac gtgctcgtca tgcagacgct ctcgcagggg
      301 acggaagcgt tgaagccgat gctcgtcgag ttgttggtcg aagagttgtc cccgcgcgcg
      361 gtcgtcgagc gcaacgacgt gcgcgtgcgc gcgctcgaag ggttggagct tgagtcgggc
      421 gtgctctacg gcgacgcgcc tgacttgatg gagattgtgc agcacggcgt ccgcttccgc
      481 gtgtcgccgc tcggcgggca gaagaccggc gcgtttctcg accagcgcga aaatcacctc
      541 gccgcccgca cacacgcacg cgggcgtgcg ctcgactgct tcaccttcga cggaggcttc
      601 gcgctgaact tggcgagcgc ctgcgagtcg gtcacggggc tggacgtctc ggcggaggcg
      661 acggccgccg cgcggcgcaa cgccgaactc aacggcgccg ccaacgtcga gttccgcgag
      721 gcgaacgtct tcgacgcgct gcgtgagatg gagtcttcgg gtgcgcgctt cgacaccgtc
      781 gtcctcgacc cgcccgcctt cgccaagaac cgcgcgagca tccccgcggc cgcgcgcggc
      841 tacaaggaga tcaacctgcg cgcgctcaag ctgctcgcgc gcggcggcgt gctcgtcacc
      901 tgcacgtgct cctaccacat gagcgagggg atgtttctgg aggtgattgc ggaagcagcg
      961 gccgacgccc atcggcgctt gcagatcgtc gagcgacgca cgcagtcagc cgaccacccc
     1021 gtcctcgtcg gcgtgcccga gacgcattat ctgaagtgca tcatcgcccg agtcatagaa
     1081 tgagggttaa ggggtaaagg ggaaggggta aggggaaaga caagttcttc tcccttaccc
     1141 cttccccttt accccttaac ccttttcctc acggcatcgt cgtcgcgaac gcctgccgct
     1201 tgattccgct cacgcaggaa gcatccagcc ccttcgccgt cccgcgctcg acaaactcga
     1261 tcatcagacg gcggatgcag tcccgattct gtagaccgcc gaagccgtgg ccgccctgcg
     1321 gcacgacgag gtgcgtgctg tttgaaagac tcttcgccac ctcgtcgccc tgcgagggcg
     1381 gcgtcacggg gtcccactgc cctgtcagga tgagcacggg cgcgtccgag cgggtcggct
     1441 gcgagtagtc ggcgggcacc ggcgagcggg gccagagggc gcaggcggcg cgctgttcgg
     1501 tgagccggta gtggccgatg aaggtgtgtt cggtgcgctc gccctcgccg ggcttgatga
     1561 aggggaggtc ttcggcgcag gtgatcgaga ggtagaggcc gttgctgtcg gcgtccacga
     1621 ggttgcgtcg gtagttgagg gctgactcgg cgagtggttt gaagtcgccg cccgccgcgc
     1681 gatggatgaa gagggggaca cgcggggaag cgcccgagtt gtagagcatg tagcggacgg
     1741 cctcggcggc gaggttgcgc gagagtttga cgcgcgccgt cgcgcccgtc tgacggagtt
     1801 tgatttcgac ttcgacgggg cccttcttta gtttctcgaa cacttcgcgc gcctctgcgc
     1861 ggacgcgagg gaaggccgcg cggcacgcct cgtcgcgctc gcactcgccg atgacgccgt
     1921 cgagggcgcg ctgcgcggac acggggaagt cgagcggcat gaagcggtgc gtcggggcga
     1981 cgccttgcaa cagcaggctg cggacgctgc gcgggtggcg cttgagatag acgagcgcgg
     2041 cgcgcgtgcc gtaggacgcc ccgaagagat ttatccgttc gtagccgagc gcggcgcgca
     2101 cctcgtcgag gtcgtcggcc gcgacgggcg tggtgtagag cgtgaggtcg gccttcgctt
     2161 cgagcgcggt gcggcagcgg cgcacggact cgggcgggaa gaactcgcgc agggcgctct
     2221 gcggatcggc cgggtcatac atcgggcaga cgagcgggtt cgagccgccg gtgccgcgct
     2281 ggtcaacaaa gacgaggtcg cgacgctcgc gcaaggaggc gaactcctgc gcgatgtagc
     2341 tggccgagtc gattgccgaa acgccggggc cgccttcgag gtagaagaag gcgtcgggcg
     2401 cgcgctccgt ccccgtggcc gggagcacga cgatcttcag accgatcttg cggccctgtc
     2461 gtgtcacgcg gttctcgaag acctcgtaga tgccgcagcg cgccttgccc ttcacgtcgg
     2521 gcacgtcgca gtcggcgagc gtgaccgacg ggcgcgcggc ctgcgccgcg tcctgcatga
     2581 cgacgcggga cgccggcgca ggccacgtga acagagaggc gaggagcagg gcgagtgtgg
     2641 tgagagaaaa catgactctc cttcaacgat gccttcatct tccgttgcgg ccggcgcggg
     2701 actcgttcac ttcgccgacg agagcgcctt cacgagtcgg tagaggaatt cctggctgtc
     2761 gtagagttcc ttgacgccga cacgctcgtc gcggccgtgc gcgcggttgt cgtcgatgtc
     2821 ggagaagagg ccggagacgc cgtacatggg gacgccggcc tgccggaagt agaggctgtc
     2881 ggtggcgccc gtgcccatga cggggatgac ggggacaccg ggccacatct cggcggtgag
     2941 actctcgatg ggctgcatga cctcggcgcg caggggcgac ggggaactcg gcttcgactg
     3001 cgcgattgcc gcgaccttga tctggtcgtc ggcgagaatg cgcgtgagcg tgcgctccac
     3061 gtcggcggcc gactcctgcg ggaggatgcg gcagttgacg gtggcgcgcg ccgtctgcgg
     3121 cagggcgttc tcggcgtggc cgcccgtgag catcgtcggg acgcaggtgg tccgcatcag
     3181 cgcgttgtag tagggggatt ttgaagcgag ccgctcggcg gccgacgggt cggggcgctc
     3241 gttcgcgacc gccgccatgt cggccaccgc cggccccttc tcaagcttcg acatgcgcgc
     3301 gaagtaggcg cgtgtcacct cacccagatt gacggggaag tcgaacttcg agaggcggtc
     3361 gagtccggcg gcgaggcggt agatggcgtt gtcgcggtcg ggcagcgaac tgtggccgcc
     3421 cttgttttta acttcgagcg tgaagctctg gaagaccttc tcgctggcct gcacgccgtt
     3481 gaagaggggc gtgccctgac gcgagcgccc gccggcgccc tcgttgatgc caaactcggc
     3541 gtcaaccaaa gcgcggtgct ccttgagcag aaactcgacg ccgttgaagt cgccgccttc
     3601 ctcgtcggcc gtcagcgcga cgatgaggtc gcgctcgggt ttgaagttct cctgcttgta
     3661 gcggatgagg ttggcgatga agacggcggc catcgctttg tcgtcgccgg tgccgcggcc
     3721 gtagaagtag ccttcgcgct cggtgagctt gaaggggtcg agaccatcag accagtcctc
     3781 cttgagggcc tcgaccacgt cgatgtgcgc gagcagaagg agaggcttct tcgcgcccgt
     3841 gccgcgcagg cgcgcgacca gatttccttt gcgctcgttg gccgtgggga cgacgacgcg
     3901 cacgtcccct tccgcgaagc cggcggcgcg caggcgcgcg gccatcgcgc gtgcggcgcg
     3961 cgtgttgtcg ccggccggcg tgtcggtggt gttgatctcg accagctcct tgtaaatgtc
     4021 tcggaggagt tgctggtgcg gcgtgagctg cacggcttgc gcgcgcacgg ggacggcccc
     4081 tgcggcgagg agcgcgagcg caaggagggc ggcgctcgtc cgccggagtg cggccgtcga
     4141 gaggccgtgg gcgcgtgatg tgtcggtcat acggtcgtcg gttccttttg ttcgcccggc
     4201 gaccatgcgc ccaatgcttg tcggagcgcg aggattttgc cgaggtggtg ggcgttgtgt
     4261 acggccagac tgcggagcgc gtcggccatt gtgaagtcgg ggtcgatttc tgcggccagc
     4321 ttctcgggcg agtcggccca ttcgagcgcc ctgtcgagtc cggctaaaaa ttcttcgacg
     4381 agggctgacc actcgcgctc ggacgcgggc gcgtccggcg ggtagacctc gccccgctgc
     4441 cacgcctcgt cctcctcgtc gcggttaacg acgatgttct gccacctcgt cgtgtgccac
     4501 agttcttcgt agatggtgtg cgactgaggc gacagccggc gcgtaacctg ctccagcgtc
     4561 agccccgaaa gaatccgttc gcgtgcggcg aactcgccgt caacgaaaag atgtttccac
     4621 ttgttcaatc gatctctccc actcggccgc ccggtctcgc tcgaggggcg cgccgctcgg
     4681 ccgcgcgtca ggttccgtta aagcccgcgc gcatctggcg gaggttggcg ccggggtact
     4741 tgccggtggc cttaaccttc tcaagcatcg cggcggcggc agcgcgggtg cggccggcgt
     4801 agacggccat gaagcgttgc ttcgggtcgg tgttggtgta gtagagatcg cccggctcca
     4861 tgcaacgcga ggcgaagacc ttgttgcgcg ggaagatcgc ttgcgcttcg agccggtctt
     4921 cttccgggat gaatcgctca cactcgtcga actcgtcctt gacgcgcatg ctccgcagga
     4981 tgatcgcgta gaaaggctcg gtttcccaga tgatggcgcg ggccgggatt tggaaaggga
     5041 tgttgtaggg cggaaattct atctggccgg tgcggcagcg cacggtcggg tcgccgcaga
     5101 ccacggccct gccgcgcttc tgcgcgatgg ccgagggcgg cgcgatggtg gcggcggcgg
     5161 cggcgtagag gaggacggtg gcgaggagaa cgaggagcgt gacggacgac gtgtgtgtgt
     5221 gtctcatctt tggtggcgct cgggggtttc agtttgtcgc ggcggcgcgg ccgatgtttt
     5281 cggccaactg ttttgcctcg gtgaagagtt cgagggcgcg caggagttgc gggtcgcctt
     5341 cgaggatgac gcgcgcgccg gcgtcgccgc cgaagctggc ggtgacgagg tcttcacgca
     5401 gacgcaggcg gacgtattca acttcggcgt caacctgcgc cgtcgtcagg gcgtgcgccg
     5461 ggtcgcgccg gacgaactcg cggaaggctt cgacgacgcg gtcgttgata gtgaagtcgg
     5521 tcgggcgcgg gaagcggccg aactgcggct cggcgggcgc gcggaaattc tcgaagccgg
     5581 gagcctgtcc ggcggcgagc agacgcgcga aatgaaacgt cgcgtcgaag acgcggccgc
     5641 gcaggggcgt gttgacgtta cggggcttga tgccgtggtc gggcgcgatg ccgccgccgc
     5701 cgtagaagac gcgaccgccc gcggccgtga cggccgtccc cgtgggcgtg ggttgcggcg
     5761 cgggtgccgc ctcggggccg ggcggcgcgg cctgcgctgg tgaaagggga agggcgcttt
     5821 gctgttcgtc ctcttcgtcg cggcgcgtga cgtagtcgta gtaggagccg ctggtgtagt
     5881 tgcgttggat gagacggccg tagggcgtgt agtacttcgc cgtcgtgagc gtgaggcccg
     5941 cgccgtaggg gagcgggtag accttctgca cgagtccctt gccgaaggag gtttcgccga
     6001 cgacgaggcc gcgcccgtga tcctgcaccg ccccggcgac gatctcggcg gccgaggccg
     6061 agttgcggtt gatgagcacg acgagcgggt agtcaacggg gtcagtgccc acgcttcggt
     6121 agacgcgctc gcgctcgccc tcgcggccgc gcaccgagac gatggtcttg ccgcgctcga
     6181 tgaagaggct ggcgacgctg atggactggt cgagcagacc gccggggttg ttgcgaaggt
     6241 ctaagacgag ctggcgcatg ccctcagtgc ggagtcgttc gagcgcctcg cgcagctcgt
     6301 ccgtcgtctt gtgattgaag ctcccgaggg cgacgtagcc ggtgccgggg cggacgatgt
     6361 aggcgtcgcg gacggacgac tgcgggacgg aggcgcgcac gatctggaag tgctccggca
     6421 cttcggagcc ggcgcgcatg atcttgagcg agaccggctt gcccggctcg ccgcgcacgg
     6481 ccttcgccgt ctgcgccgtc gtccactcgc gcgcgtcctg accgtcaact tcggcgatga
     6541 ggtcgccgaa gcgcaggccg gcgcgtgcgg ccggcgtgtc gggcagcggc gactgcacgt
     6601 agacgccgtc gcggtggcgc aaaatcgaga cgccgatgcc gacatagcgc gcctcctgat
     6661 cctgcatcag acgcgtgtac tcggccggcg cgaagtagtt cgagtgcggg tcgagggcga
     6721 ggagcatgcc ctgaatggcc gcgtggctcg ccttctcgta gtcgatctcc tcggcgtact
     6781 cgccggcgac gacgccgagc gcctccgtgt acgacccctc gaccgccgcg gccgggccgg
     6841 tagcgttccg ccccgcgagg agcgggttcg aggcgaagcc gccgatgatg ccgcccagaa
     6901 ggctccccag ggcgacgacg aagacggccg cgatgcgcca gaaagttcta ctgctcatag
     6961 cttgaaaacg gtgatgagtg ataagtgata agtgatgaga cgtacaagag gctcacacgt
     7021 catcagtctt tcgcccgcca cgcgcggaaa gattttaaca cagccccgcg cccgcccgcc
     7081 cgcggcgagc gccgcacgcc gcgatgtgct ataaaaacgg tgataagtga aaagtgatga
     7141 gtgataagaa agagggagcg cgtccgcctc ttccttctta tcactcatca ctcatcactc
     7201 atcacttttt tttgaggtta acgatgtcgt cgctacttct ctccgtagtc atccccgcct
     7261 acaacgaggc gccgagactg ggccggacgc tcgatcaggt gctggcgtat ctgaacgagc
     7321 agcagtacga gagcgagctg atcgtggtgg acgatggctc gcgcgatgag accgtggccg
     7381 tggccgaggg ccgcttcgag ggcgcggcgc ggggccgcgt cgccacgcgt gtcatccgcg
     7441 tcgagccaaa ccgtgggaag ggcaacgccg tgcggacggg gcttttggcg gcgcgcggcc
     7501 gcgtcgccct cttctccgac gccgacctct ccacgcccat caccgaagcg ccgaagctga
     7561 cggggccgat ccgctcgggg cagtacgata tcgtcttcgg ctcgcgcgcg ctcgaccgca
     7621 gcctcatcgg cgtccaccag ccttggcagc gcgagcagag cgggcgcgtc ttcaatctct
     7681 tgatgcggct ggtgacgggg ctgcccttct gggacacgca gtgcggcttc aaggcgttcc
     7741 gcatggacgt gtgccgcgcg gtcgtcgagg gcgccgtgat cgaccgcttc gggttcgacg
     7801 tggagctgct ctacatcgcg caccgctcgg gggtgcgact gcttgagcgc gccgtgcgct
     7861 gggacgacgc ggccggcagc aaggtcagct tccgttcggg cctgcaaggc ttcgacgaac
     7921 tgcgccagct gcgccgcaac agccgccgcg gagtctacga cgaagccatg gcgcgcgccc
     7981 gcgagcgggc agagcgcgtc cgcgacgccg gcccgctcct cctgcccggc gaagcccctc
     8041 ccgaaacaga cgcctcccct gaactcgccg ccgcgcgcag aatctaaacg ccagcccgtc
     8101 gcgccagccc gccgcgtcac acgccgcgcc cgcgccttcc tctgcacgca cgcagtccgc
     8161 aaggattggc ccgcgcggcg gcggcctaaa tggcacttgc gcgtccgccg tcaagggtta
     8221 cgattccaat cgtctttgtc tccgaagttt tcatccgtga ggtgtgtcaa tggtcgcacg
     8281 tctgaacgtc aacatcgatc acgtcgccac cgtgcggcag gcgcgaaggg cgccggagcc
     8341 gagcctgatc gcggcggccg cgctgtgcga gagcgcgggg gccgacggca tcaccgtcca
     8401 cctgcggggc gaccgccggc acattcagga cgaagacctc cgcacgctgc ggcgcacgct
     8461 cacgacctac ctcaacgccg agatggccgc caccgacgag atgttgaaga tcgcgctcga
     8521 cgtgcggccc gactgcgtga cgctcgtcgc cgaacgcgct gacgagttga cgaccgaggg
     8581 cggcatggac gtgcgcgctg gcgtggagac ggtgcgcgcg gccgtcggag tcttgcgcga
     8641 gggcggcatc ctgacgagcc tttttatcga ccccgaggcc gcgcagatcg aggccgcgcg
     8701 cgcgtccggc gccgagcagg tggaactctg cacggccgcc tacgcggcgg cgactctggg
     8761 cggtctcgcc gtccacacgg gggggcgtgc cgcggaggag ctgcaacgac tgcgcgaggc
     8821 cgcccgcctc gcggcgcagc acggcctaca ggtcgccgcc ggccatggcc tcacctatcg
     8881 caacgtcggc gccgtcgccg ccatccgcga gatcgtcgag ttcaacatcg gccacaacgt
     8941 cgtcgcgcgc gccgtcctcg tcggcatgga cggggccgtg cgagagatgc gcgaacgcat
     9001 cagggaaggg gccatgggaa cacaatgaac atggacagga ttgacaggac gaacaggatg
     9061 agaagatttc aaatcacacg acacacatcg cacatctgaa atttcgactc cgacatttgt
     9121 catctgccat ctgctttttc ttcatcctgt tcgtcctgtc aatcctgtcc attcctcccg
     9181 aagccggcgc gcgtttgaca tcgccgcgcc ggttctttac aagttgtccg aagtcttccc
     9241 cacgacgttc aaccaagccg aaaagtattt cgagaccgaa caggagcgcc cacgtgacct
     9301 ctcgcgtcgc cggagcagcc ggcccacgtt ttctcttcgc cacagccttc gcctgcctcg
     9361 cgctctgcct cgccgcgcct gacgccttcg cgcaacagcc gtctgcgtca cccacgcccg
     9421 ccgccacgcc cgatgtgccg gccctcgcga aaatcgagtt catcggtctg tcgcgtgtga
     9481 aacaggagga ggcgacggag gcgacggggt tgcaggcggg ccagcccata gacgttgacc
     9541 tcgtggacgc ggcggccgcg cgcctgctcg actcgggact cttccaaaaa ctcagctaca
     9601 gctaccgcgc cgcgaagggg caggccacgg tcgtcttcac ggtcgaggag atgggcgcgc
     9661 gcgcgcccgt cgtcttcgac aacttcgtct ggttcagcga ccgggagatg gacgagtacc
     9721 tgcgtaagag catctcgggc tacgacggca cggccgccga cgcgggcacg atgaccgaga
     9781 agatcgccaa ggcgctcggc gagatgctgc tgctcaaggg catcaagggg cgggtcgagt
     9841 atcgcccttc ggccgaccct tcggggcgca accccacgca cctcttcgcc gtccgcgacg
     9901 cgacggggct gcgtgtctgc tcaatcagct tccccggcgc ggcggccgtc agggagcagg
     9961 tgctcgtgca gaactcgggc aggctcacgt cggaggaatt ctcgcaacag tccgcactcg
    10021 ccttcgccga gacgaatctg ctgccgctct accgcgagcg cggcaacctg cgcgccgcct
    10081 tccgcgctcc gcaggcgcgg cccgcgaccg aagacgactg cctcggagtc gccgtgagtt
    10141 ttccggtcga tgagggcatc gcctacgtct gggagaaggc ggagtggtgg ggcctcgaat
    10201 cgctgccggc cgcggcgctc gacgccgcgc tcggcatgag gccgcgcgaa ctcgtcaacc
    10261 tctcgaagat cgagaagggg ctggaggcgg cgcgcaagct ctatgcgcgc aagggccatt
    10321 tggcgccgaa actccagccc actttcgtct tcgacgacgc ggcgcgcagc gtcacctacc
    10381 gcgtcgtcgt cgtggagggg ccgcagtatc gcatgggcga actcttcatc acgggcctct
    10441 ccgagaagga caccaacaac gtccgcaccc gctggagcct gcccccgcgc gagccttacg
    10501 acgccgagta ccttgacgag tttctgaaga aggccgtcgc ggagttcctg cgcgacccct
    10561 cgaccgacgc gcgcgccctc ggcaagctca aggtcgatac caacgtcagg cccgacaagg
    10621 agaagttgat cgttgacgta agcatcgact tcaaacagca gaagtaatct ccacgcgttt
    10681 tcagcaaccc ttccacaaaa acacgtcggg cgggcaagcg aggaagactc ccgatccgtc
    10741 cccgccaacc cgcccggcgc ttttttcggc ctcacgtcac gcgaaaggac cgaaaagttg
    10801 aaagcggtta gctcatagct gttagctcac ggcatacgcc gaaggacgcg ccgctgctta
    10861 atactcaaac actccgcttc ttcgcttctt catagctaac agctaacagc taatagctaa
    10921 ccgcttcttt taactcgcca tcgccccgcg ctcggacttc ggcacgggga agccgaggtg
    10981 cttgtaggcg gcgcgcgtga ccatgcggcc gcggggcgtg cggttgagga agccgatctg
    11041 aatcaggtac ggctcgatga tctcctcgat gctttcgcgc tcctcgctga tggaggccga
    11101 gatggtgccg acgccgacgg ggccgccgtc gaacttctcg atgatcgtcg tcagaacctt
    11161 gcggtcgatc tcgtcgaggc cgaagcggtc aacctccatc tcgtcgagcg cgcgacgggc
    11221 gagcgcctgc gtgatgcgcc cgtcggccag cacctcggcg tagtcgcgca cccgcctgag
    11281 cagacggttg gcgatacgcg gggtgccccg cgcccgacgc gcgatctcgc ccgcgcccga
    11341 cgcctcgatc tccacgttca aaatctccgc cgagcggtgg acgatgatct ccaaatcctt
    11401 ccgcgaataa aaatcgaggt ggaagacgat gccgaagcgt ccgcgcaagg gcgcggtgat
    11461 gagtccggcg cgggtcgtcg cgccgactaa ggtgaacttc ggcaggtcta acttgatgga
    11521 gcgggcggcc gcgccctgtc cgatcatcag gtcgagctga tagtcctcca tcgccgggta
    11581 gagcacttcc tcgatctgcg gcttgaggcg atgaatctcg tcgatgaaga ggacttcgcc
    11641 ctcttgaaga ttagtgagca ccgccgccaa atcgccgccg cgctcgaccg ccggcgcgct
    11701 cacgcacttg agcggcgcgt ccatctcgtg cgcgatgatt tgcgacaggg tggtcttgcc
    11761 cagacccggc gggccgaaga agagggcgtg gtcgagacat tcgcggcggg ccttagcggc
    11821 gcgcagaaaa atcttgagat tggtcgtgat cttcggctgg ccgatgtact cgtcgagact
    11881 ccggggccgt agcgatagct cgacggcctg atcttcgggc gtcgccgcac ccgcaatctc
    11941 gcgcggcgcg tccgccatcg gcgcttcaga aatggcttgt gacatgaaag aaaaccccct
    12001 cgtaaacgcg agcctgacga ctctccgatt gagcggccat tctaccatgc gcgggccggc
    12061 gtttcaagtg tgaaacacga agaagagagg gatttttatc ggagtgagca agcgagcgcg
    12121 gtgtgtttac cgcgggttgt cggcggcgag ttggaggcgg gcggtggcgc gttcgaggtc
    12181 ggtgcgaagg gcggccatct cctcgggcgt gccggcgaag gcgccgagac ggcgctcggc
    12241 ctcctcgcgc tcgcggcggg cgttctgcgt atcgacttct tcggggaact cggccatgtc
    12301 ggcgagcacg gagacgcggt cggcgttgac ctcgacgaag ccgccggaga cgagcaggcg
    12361 gcgcgtcgtc cccgattgcg cgtaggagag cacgccggtc tggagctgcg agatgagcgg
    12421 cgtgtggccg ggcaggatgc ccagctcacc gcccacgccg ggcaggttta cggaatcgac
    12481 cgactcggag acgagcagcc gctcgggcgt gatgacatcg agttggaggg gcatagtcag
    12541 tgataagtga taagtgatga gtgataagtg ataagaaaga gggaagcgga ttttcttatc
    12601 acttatcact catcacttat cactactttt cctatccgag ttccgcggcc atgcgttcgg
    12661 ctttctggcg ggcctcttcg atggtgccga ccatgtagaa ggcctgctcg gggaggtcgt
    12721 cgtgtttgcc gtcggcgatc tcctggaagc cgcggatggt gtcggccgtc ttgacgaagg
    12781 cgcccttgag gccggtgaac tgttcggcga cgaagaaggg ctgcgagagg aacttctgaa
    12841 tcttgcgcgc gcgcgagacc accagcctgt cttcctcgga gagttcgtct aagccgagga
    12901 tggcgatgat gtcctgaagg tctttgtagc gctgcaaaat ctgcttcacg cgctgcgccg
    12961 tgttgtagtg ctcctcgccg acgatgcgcg ggtcgaggat gcgcgaactc gacgtgagcg
    13021 gatcgacggc cgggtagatg cccagctcga cgatctgacg cgaaagcgcc gtgacggcgt
    13081 cgaggtgcgc gaaggtcgtg gccggcgcgg ggtcggtgta gtcgtcggcc gggacgtaga
    13141 cggcttgaat cgaggtgatc gcgccggtgc gcgtcgaggt gatgcgctcc tgcaactcgc
    13201 ccatctcggt ggcgaggttg ggctggtagc cgacggccga gggcatgcgg ccgagcaggg
    13261 ccgacacttc ggagccggcc tgcgtgaagc ggaagatgtt atcgacgaag aggagcacgt
    13321 cgagcccctg atcgcggaag tactcggcga cggtgaggcc ggagagggcc acgcgcaggc
    13381 gcgctcccgg cggctcggtc atctgcccgt agacgagcgc caccttcgac tcggggacgg
    13441 agcgcatgtc caccttcgtc atgtcgaaga cgcccgtctc ctcgtagctt tcgtagaagc
    13501 tgtcgccgaa gttgatgacc ttcgactcga ccatctcgcg cagcaggtcg ttgccctcgc
    13561 gcgtgcgctc gccgacgccg gcgaagaccg agaagccgga gtgggccttg gcgacgttat
    13621 tgatcagctc catgatgagc acggtcttgc cgacgcccgc gccgccgaac aaaccgatct
    13681 tgccgccgcg caggaagggc tggacgagat cgacgacctt gatgccggtc tcgaacatct
    13741 ggagttcggt tgactgctcg gcgagcaggg gcgcgtggcg gtgaatcggg taacgctgct
    13801 cggagtggac ggggccgagg ttatcgacgg gctgaccgat gacgttcagg atacgcccga
    13861 gcgtggactc gccgacgggc atcgtaatcg gcccgccggt gtcgatggcc gtcatgccgc
    13921 gcaccaaccc ttcggtgggc tgcatcgaga cggtgcggac gcgtccctcg ccgagatgct
    13981 gttcgacctc ggcgatgatg tcgatgggcg tgggcacgtc gaagccctcg gaggtgatcc
    14041 gcagagcttg atagatgggc ggcaggtagc catcggtgaa ctccacgtcc atgacggggc
    14101 cgatgatctg cacgacccgg cccgtctgcc cgctgctgcc tgccgtgtt
//
LOCUS       DASVES010000195         5036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_407226, whole genome shotgun sequence.
ACCESSION   DASVES010000195 DASVES010000000
VERSION     DASVES010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407226"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VF240_07740"
     CDS             complement(<1..662)
                     /locus_tag="VF240_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175159.1"
                     /translation="MLTSTHTHPARVGDWCEPLRRFDISRLWAELQRLVSNHPLVRAS
                     HSAGLLVEEGDRGSAYIDLTQELFVTLLAKSRFQHYLDTAMTDSEIECEIGQIELTNL
                     LTAELRKRHPESYRLARRISTIIQSSANFRRCDTAGGGEATGDEPHRRLVDRVYGLRE
                     WRGDKQRRGQHELEQRMQIIAVRQRDTRMVGCTGDAQIIISNSDLEDLIVSVLEACDS
                     PA"
     gene            complement(1066..1458)
                     /locus_tag="VF240_07745"
     CDS             complement(1066..1458)
                     /locus_tag="VF240_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175160.1"
                     /translation="MYPQKKRILCVDDDGDTRYLLKILLDRSDLETVCVGNVAAALRL
                     MGTEQFRLYVIDGQLPGVSGLTMCEEIRRRDRQTPIVIFSGQAYADDREAGLRAGANV
                     YIVKPDTGQLVPAVRRLLEEAEAAAMSR"
     gene            complement(1878..2039)
                     /locus_tag="VF240_07750"
     CDS             complement(1878..2039)
                     /locus_tag="VF240_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7175161.1"
                     /translation="MTDREAEVLRLIALGYINKEVAARLSLSVTTVEAHKARGMEKLG
                     MKSRVDLVR"
     gene            complement(2032..2268)
                     /locus_tag="VF240_07755"
     CDS             complement(2032..2268)
                     /locus_tag="VF240_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175162.1"
                     /translation="MRVCSPRLPVAPIVHALNDEQARARARGAVAAGRPERGDEAPMV
                     CKHLFAAAVVHGALSRMDSRAMGRSTRICRNEVD"
     gene            2502..3032
                     /locus_tag="VF240_07760"
     CDS             2502..3032
                     /locus_tag="VF240_07760"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7175163.1"
                     /translation="MRRFLITAALCLCAAPAAAQQPTTPAQTPAQPATQKPAGEKVGM
                     AGAEQAVAGRVSAFLAALRKGDEATLAPFYADDYTITMDTGAVQTKAERLAWVKANTA
                     RLSTLDFQDLKTRVYGDTAVVTGHAIGQGEGDAKVNSRMIQVWVKQGGEWRLVGGQTT
                     AIAPAQPATAPAEKKP"
     gene            3677..4066
                     /locus_tag="VF240_07765"
     CDS             3677..4066
                     /locus_tag="VF240_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175164.1"
                     /translation="MAYRVLYVEDHDDTRVIVTILLERAGFEVAAVSCGSEALELARA
                     GRFDLYLLDHTFSDASGITVCRGIREFDAGTPILFYSARAMEQEIEEALGAGAQGYLV
                     KPLDIFNVAEHASRLIAEARRGREGGQ"
     gene            4148..4585
                     /locus_tag="VF240_07770"
     CDS             4148..4585
                     /locus_tag="VF240_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175165.1"
                     /translation="MDGKEGADRTILVVEDHDDTRALLRRLLEHHGYGVAEASDGRRA
                     VEAVRLRCPDLILMDLHLPLLDGEGATRLIRELSGVCPVPIIAVTALDAEEHRRRAVL
                     AGCNEYVEKPPDPELLLAKIATLLEAGRAARLAERMAAAMHAG"
BASE COUNT          916 a         1616 c         1630 g          874 t
ORIGIN      
        1 tcggccggcg agtcgcaggc ttcgaggacg gagacgatca agtcttcgag gtcggagttg
       61 ctgatgatga tctgcgcgtc gcccgtgcag ccgaccatgc gcgtgtcgcg ctggcgcacg
      121 gcgatgatct gcatgcgctg ttcgagttcg tgctggccgc gccgctgctt gtcgccgcgc
      181 cactcgcgca gcccgtagac gcggtcaacc aggcgccggt gcggctcgtc gcccgtggcc
      241 tcgccgccgc cggccgtgtc gcagcggcgg aagttggccg acgactggat gatggtcgag
      301 atgcgccggg cgagccggta gctttcgggg tggcgcttgc gcagctcggc cgtcaacagg
      361 ttcgtcagct cgatctgtcc gatctcgcac tcgatctccg agtcggtcat cgccgtgtcg
      421 aggtagtgct ggaagcgcga cttggcgaga agggtgacga acaactcctg cgtcagatcg
      481 atgtaggcgc tgccgcggtc gccctcctcg acgagcagcc cggcgctgtg cgaggcgcgc
      541 accagcgggt ggttgctcac gaggcgctgc aactccgccc agaggcggga gatatcaaag
      601 cggcgcaacg gctcgcacca gtcgcctacc cgcgccggat gagtgtgcgt gctagttaac
      661 atgcgccccg ggttcccgaa gtgaaggtca aacgccctct ctcacgatct gcgaaatcaa
      721 agctgccttt tcatcagtga ggtctgcgaa ccgcaggccg aatcccagcg gccactcgcg
      781 gtgagctacc gaacattcca gatggagatg tgcctgcgac atcaggatcg cgtccaccct
      841 gatgtgttca tcaacactga ccctcccgac gctattgatg taacacccgc cccgactaac
      901 atcggtcatc agtgcatagt tgttgattga taacccctcc cacaggacga gtagctttgt
      961 aggtacacgc tgatgggacc ttttctcgtg gggcatgttc gtcacagccg ccgccggtcg
     1021 tcattccgcc ctccgccggc ggacgacaag tcgccgcgtc aagcattagc ggctcatcgc
     1081 ggcggcctcg gcctcctcca atagccgcct gacagctggc acgagctggc cggtgtccgg
     1141 cttgacgatg tagacgttgg cgcccgcacg cagccccgcc tcgcggtcat ccgcgtaggc
     1201 ttggccggag aagataacga tgggcgtctg cctgtcgcgc ctcctgatct cctcgcacat
     1261 cgtcagcccg gagacgccgg gcagctggcc gtcgatgacg tagagacgga attgctccgt
     1321 ccccatgagc cgcagggcgg cggccacgtt ccccacgcac accgtctcca agtccgaacg
     1381 gtccaggagg attttgagca ggtagcgggt gtccccgtca tcatcgacgc agaggatacg
     1441 ttttttctgt gggtacattg tcgcctcccg accggcatcg acacgcctgc aactcggccc
     1501 cggcacccgg tttgactaga taggcattaa cgccccctct tttctcggtt gatgaaaagc
     1561 taatcgggct tcttgatatg cacgagcaat gattttgtgc ggtcgcagtt tgtacgcctg
     1621 ctgacacgcg tcaaaaggac tctcaccgga actgctccgt cttcgtaagc gcagttaaag
     1681 acaaagctgt tcgttccgcc gtctcgtccc ggcactacgc agacgaaaca aacaaccccc
     1741 aaacgcttct ccggtattgc caaagaaggt ggcggttatg ccttgcgggt taatgattgt
     1801 agttttaact tacgtgatga gttttggata tcaggagggt gggtgaaagt ggcaagggtt
     1861 ttaccctgca gcatcgccta gcgcacgagg tcaacgcgac ttttcatgcc cagcttctcc
     1921 atccctcgcg ccttgtgagc ttcgacggtt gtgacgctca gggagaggcg tgcggccacc
     1981 tctttgttga tgtagcccaa cgcgatgagg cgcaggactt ccgcctcccg gtcagtcaac
     2041 ttcgtttcgg cagatgcgcg tcgagcggcc catcgcgcga gagtccatcc gcgagagcgc
     2101 cccgtggacg acggccgcgg cgaataagtg cttgcagacc atcggcgcct cgtcgccgcg
     2161 ctcgggcctg cccgcggcga ctgccccgcg tgcgcgcgcc cgcgcctgct cgtcattgag
     2221 cgcgtggaca attggggcga ccgggaggcg tggtgaacat acgcgcacgg ggccgccgaa
     2281 aggcgaagtg ttacggcggc cgggtggcga caagagcgcc gcgtcttata gcacgggctg
     2341 gtgaagacac aaaatattcc ctcttagata acacttcaac aactctcaaa actttgcgca
     2401 gaatcgccct ctgttgaatc tggggtacag ccggccattg actccgcgcg cgcgttggga
     2461 tagaagcacc tcatcaatcc agctaccgaa aggaaacgac catgagaaga tttctgatca
     2521 ctgccgcgct gtgcctgtgc gcagccccgg ccgccgcgca gcagccgacc accccggcgc
     2581 aaaccccggc gcagcccgcc acgcagaagc ccgccggcga aaaggtcggc atggccggcg
     2641 ccgagcaggc cgtggcgggc cgggtgagcg cgttcctggc cgccctgcgc aagggcgacg
     2701 aggcgacgct cgccccgttc tacgccgacg actacaccat caccatggac accggcgccg
     2761 tgcagacgaa ggcggagcgc ctagcgtggg tcaaggcgaa cacggcgcgg ctgagcacgc
     2821 tcgacttcca ggacctgaag acgcgcgtct acggcgacac ggcggtcgtc accggccacg
     2881 ccatagggca aggcgagggc gacgcgaagg tcaacagccg aatgatccag gtgtgggtca
     2941 agcagggcgg cgagtggcgg ttggttggcg ggcagaccac ggccatcgcc ccggcgcagc
     3001 cggccacggc gccggccgag aagaagcctt aagggcggca taccatcccc ttttattgca
     3061 tcaccaaaag tgcgcgagag ccttacgtca tttccgccct cacgcgagag tgtaagaaaa
     3121 gtgggcggct gaacttgtcg cttcatcaca cgcgctaacg acgtggccga ctcgtggagg
     3181 gaaggggaac acccccgccc tctttcgagt cggccttttt catataaagc cgtgagtgag
     3241 ggcggggcgc ggggtgctct ccccaacgcc ccgcaacccc gcccctgacg cgacaacgcc
     3301 ccgcggccat tggacccgat gcggcgggcc gcaggcagag actgggcgct tagcgtcaac
     3361 ctgggtttac aagcggcgtg ccgcggagga cttcgtgggc gcccatgctt acgactccgg
     3421 ccatggtgaa gacttcgacg gggatggacg catgggccgc gcggcgctaa ttactcaccc
     3481 cagacggcaa atggcgggcg tggcatgagt aaaggggacg ctgacgtaca tacggccgcc
     3541 ccatgttatg ttatacgggt gctcacacag gagggccgac gggccgcctc ccggcgcttc
     3601 gcttgaagta aacccgaccg ctacggccgc ggcccgtcgc ccacttaaaa cattccccgg
     3661 cagaaagctg accgtcatgg cctaccgcgt cctctacgtc gaggatcatg acgacacgcg
     3721 cgtcatcgtc acgatcctgc tagagcgggc cggcttcgag gtggccgcag tgagctgcgg
     3781 tagtgaggcc ctagagttgg cccgcgccgg acgcttcgac ctctacctgc tcgaccacac
     3841 cttctctgat gcctcgggca tcaccgtttg ccggggcatc cgcgagttcg acgcggggac
     3901 acccatcctc ttctactcgg ccagggcgat ggagcaggag atcgaggagg cgctcggggc
     3961 cggggcgcag ggctacctcg tcaagccgct cgacatcttc aacgtggccg agcacgcctc
     4021 gcgcctcatt gcggaagctc gccgggggcg ggagggcggg cagtaggggc acgcgccttg
     4081 ctccccgcac ccgattaaaa ccccggccgt cacgtttcag gacggccggt gcgcgagagg
     4141 gaaagacatg gatggaaagg aaggtgcgga ccggaccatt ctcgtcgtcg aggatcacga
     4201 cgacacgcgg gcgctgctgc ggcggctgct cgaacatcac ggctacggcg tggcggaggc
     4261 gtccgacggc cggcgggcgg tcgaggcggt gcggctccgg tgccccgacc tgatcctgat
     4321 ggacctgcac ctgccgctct tagacggcga gggtgccacg cggctgatcc gcgagttgag
     4381 tggggtgtgc ccggtgccga tcatcgccgt cacagccctg gacgcggagg aacaccgccg
     4441 gcgggcggtg ctggccgggt gcaacgagta cgtagagaag ccgccggacc cggagctact
     4501 actggcaaag atcgcgacgc tgttggaggc cgggcgggcc gcgcgcctag ccgaaaggat
     4561 ggcggcggcg atgcacgcgg ggtgagcgga cgagccgaaa gccaggcaag tagggcgatt
     4621 gggtgcaaag cagaggggcg cccgcctcat taatcgtgga agaaaagggc ttggggcagc
     4681 cttgcccgca gccccgcgcc cttccctcac tggcgagggt tgaccgggtt gcgaatggtg
     4741 cctgtctctc cttaccaagc gccgcccacg accccaccga ccctctgggc ctttgagaaa
     4801 gggcgaacgg cgccggcctt ccaacccggg tgaagacttc gcatttaatc gacctctgtt
     4861 gaatcgggac gtgtcgcggg tcggactcgc ccccgaaatc gggggataaa tgcggcctca
     4921 cttttttctg gaatcactgc cggattacga gttttcctcg attgaactat tcattctctc
     4981 cccgagatgc agaaacggcc acagacaggt gcgcccgccc cgcgcgcctg tctggt
//
LOCUS       DASVES010000196         4416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1069692, whole genome shotgun sequence.
ACCESSION   DASVES010000196 DASVES010000000
VERSION     DASVES010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069692"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..538
                     /locus_tag="VF240_07775"
     CDS             74..538
                     /locus_tag="VF240_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175166.1"
                     /translation="MRLKMVDRLMPGFGRTQTLLALLVGATMVAGALVVGARSVFFPP
                     RFRGFGEVTAGGQVAGWVVDGQEETTARPVEVQLYIDGVFAAAATADLPRPDVLAAGR
                     ARDERCGYSFALPTLAAGEHEARVYAAHAVGGGAYRTLQQTGAALRFRVGQR"
     gene            630..1628
                     /gene="moaA"
                     /locus_tag="VF240_07780"
     CDS             630..1628
                     /gene="moaA"
                     /locus_tag="VF240_07780"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX7175167.1"
                     /translation="MTEAALTDSYGRAIRDLRVSVTDRCNFRCFYCLPHGEPPAAPKE
                     SMLTYEEIERAVRLFASLGVEKVRLTGGEPMLRRDIETLVGKLARVPGLSDLALTTNG
                     FNLAERAAGLKEAGLDRVTISLDSLKPERFREMTGVDVLERVLEGVRAAKRVGLVPVK
                     INAVIVRGHNEGEVADMAAFAREHGAVMRFIEYMPLDSGHGWSREQVYTGAEIRRAVA
                     ERFPLVGVGGARGSDTSTRYRFADGAPGEVGIIAPVTEPFCGACSRIRLTADGQIRTC
                     LFSKAEHSLRDVLRSGASDEETIEFIRAVVLRKEPRHYINETYFEQPARTMSLIGG"
     gene            1661..2206
                     /locus_tag="VF240_07785"
     CDS             1661..2206
                     /locus_tag="VF240_07785"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7175168.1"
                     /translation="MSTKTEATLDDLYKVEGKAELVNGEIVHMSPTGRRPSYAARAIF
                     LSLYDYEKRTGSGYAVTDGAGFAVSLPHRKSFSPDAAFYVGDMDSMKFFAGAPVFAVE
                     VRSENDYGPKAEEKIAAKRADYFAAGTRAVWDVDLLSDDVVRVYRASDPDAPMVYRRG
                     ERAEAEPAVSGWLMPVDDLFA"
     regulatory      2267..2386
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2466..4394
                     /gene="thiC"
                     /locus_tag="VF240_07790"
     CDS             2466..4394
                     /gene="thiC"
                     /locus_tag="VF240_07790"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX7175169.1"
                     /translation="MTTTKDKGEERQSDEVQFPASRRIYVAGETDGVRVPFREVSLQP
                     TRLPGGAVEENEPVRIYETAGPWADPDARCDVREGLGALRREWILARGDVEEYEGREI
                     VPQDDGYLTENAVEFARHKDRGRLEDFPGLRRPPLRARAGSAVTQMHYARRGRITPEM
                     EFVALRENMGRERALHAMREHTRDDRSSLFHEHGGERFGARIPEFVTPEFVRDEVARG
                     RAIIPANVNHPESEPMIIGRNFLVKINANIGNSAVASSIDEEVEKMRWATRWGADTVM
                     DLSTGKNIHTTREWIIRNSPVPIGTVPIYQALEKVGGRAEELTWEVFRDTLVEQAEQG
                     VDYFTIHAGVRLPFVPLTARRTTGIVSRGGAIMAKWCLARHEESFLYTRFRDICEIMR
                     AYDVSFSLGDGLRPGSIADANDEAQFAELDTLGELTQVAWELDCQVMIEGPGHVPMHL
                     VKENMDRQLAVCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKE
                     HLGLPDRQDVKEGVIAYKIAAHAADLAKGHPGAQLRDNALSKARFEFRWEDQFNLSLD
                     PVTAREFHDETLPQEGAKTAHFCSMCGPHFCSMKITEDVRKYAAEQGIAEEEALKKGM
                     EEKSKEFAAKGSELYAKA"
BASE COUNT          801 a         1443 c         1466 g          706 t
ORIGIN      
        1 ccaagctttc cggtcttcag cgcaacgcac acgcgctact cgaacagaca agcgccgatg
       61 cgaccgaaga ttgatgcgac tgaagatggt tgatcgactg atgcccggct tcggcaggac
      121 acaaacgctg ctcgcgctcc tcgtcggggc gacgatggtg gccggcgcgc tcgtggtcgg
      181 cgcgcgctcg gtcttttttc cgccgcgctt ccggggcttc ggggaggtga cggccggcgg
      241 gcaggtcgcc gggtgggtgg tggatgggca ggaggagacg acggcgcgcc cggttgaagt
      301 gcagttgtat atcgacggcg tgttcgcggc cgccgcgacg gccgacttgc cacggcccga
      361 cgtgctggcc gcgggacgcg cgcgcgacga gcgttgcggc tacagcttcg cgctcccgac
      421 gctcgccgcc ggagagcacg aggcgcgcgt ctacgcggcg cacgcggttg ggggcggcgc
      481 ctaccggacg ctccagcaga cgggcgccgc gctgcgcttc cgagttgggc agcgctgaag
      541 gttggagcgt gcgcgccgcg cggcgtatga ttgtgtttac cgaacacatc cacgcgacca
      601 tccggggcat ttgacgaaag acaagggaag tgactgaagc cgcactcaca gattcatacg
      661 ggcgagcgat acgcgatctg cgcgtgtcgg tgaccgaccg ctgcaacttc cgctgcttct
      721 actgtctgcc gcacggcgag ccgccggccg cgcccaaaga gtcgatgctc acctacgagg
      781 agatcgagcg ggcggtgcgc ctcttcgcct ccctcggcgt cgagaaggtc aggcttacgg
      841 gcggcgagcc gatgctgcgg cgcgacatcg agacgctcgt cggcaagctc gcgcgcgtcc
      901 ccggcctgag cgacctcgcg ctcacgacca acggcttcaa cctggctgag cgcgccgccg
      961 gactcaagga ggctggtctc gaccgtgtca ccatcagcct cgacagtctg aagccagaac
     1021 gcttccgcga gatgacgggt gtggacgtgc tcgaacgcgt gctcgaaggc gtccgcgccg
     1081 cgaagcgtgt cggtttagtg cccgtcaaga tcaacgccgt catcgtgcgc gggcacaacg
     1141 aaggggaggt cgccgacatg gccgccttcg cccgcgagca cggtgccgtc atgcgcttta
     1201 tcgaatacat gcccctcgac tcgggccacg gctggtcgcg cgagcaggtc tacacgggcg
     1261 cggagattcg ccgggccgtc gcggagagat tcccgctcgt cggcgtgggc ggcgcgcgcg
     1321 gctcggacac ctccacacgt tatcgctttg ccgacggcgc gcccggcgag gtcggcatca
     1381 tcgcccccgt caccgaaccc ttctgcggcg cttgctcgcg cattcgcctg acggccgacg
     1441 ggcagattcg cacctgtctt ttctcgaagg ccgaacactc tctgcgcgac gtgctgcgct
     1501 ccggcgcgtc tgacgaagag accatcgagt tcatccgcgc cgtcgtcctc cgcaaagagc
     1561 cgcgccacta catcaacgag acctacttcg agcagcccgc acgcacgatg agcctcatcg
     1621 gcggctaaag tccttttgta caaacgtggg gatttaagtt atgagcacga aaaccgaagc
     1681 tacccttgac gacctgtaca aggtcgaagg caaggccgaa ctcgtcaacg gagagatagt
     1741 ccacatgtca cccaccggac gccgcccgag ctatgcagcg agggcgattt tcctgtctct
     1801 gtacgactat gagaagcgaa ccgggagtgg ctacgccgtc accgacgggg ccgggttcgc
     1861 cgtcagcctt cctcaccgca agtctttcag cccggacgcc gccttctatg tcggggacat
     1921 ggacagtatg aagtttttcg cgggcgcgcc cgtcttcgcc gtcgaagttc ggagtgagaa
     1981 tgattacggg ccgaaggctg aagagaagat tgccgcgaag cgagccgact atttcgcggc
     2041 cggaacgcga gcggtctggg acgtggattt actgagtgat gatgttgtgc gagtttaccg
     2101 cgcgagcgac cctgacgcgc cgatggtcta ccggcgcggc gagcgggccg aggccgagcc
     2161 ggccgtgtcg ggatggctca tgccggttga tgacctgttc gcgtgagaag tcgttgcgtg
     2221 cgcgtctggc ctcgccggca gttgagggag tgctatactg cgtttgaaaa tttaacgggg
     2281 gagccgcgct accgtccggg ttaagcggct gagagtcacc ttcgggtgag accctttgaa
     2341 cctgatgcgg ttagtaccgc cgaagggagg ccgaaagaga aaccgctccg ccttcgtgcg
     2401 cgtggcggtt tttcgtttgc gggccggact attggaagcg ccggccgaaa gaaagtgtga
     2461 agcagatgac aacgacgaaa gacaaaggcg aggagcgcca aagcgacgag gtgcagttcc
     2521 ccgcctcgcg cagaatttat gtcgcgggcg agacggacgg cgtgcgcgtc cccttccgcg
     2581 aggtgagtct ccagccgacg cgtctgcccg gcggcgccgt tgaagagaac gagccggtgc
     2641 gcatctacga gacggcgggg ccgtgggccg accccgacgc gcgctgcgac gtgcgcgagg
     2701 gcttgggcgc gttgcgccgc gagtggattc tggcgcgcgg cgacgtggag gaatacgagg
     2761 ggcgcgagat cgtcccgcag gacgacgggt atctgacgga gaacgccgtc gagttcgcgc
     2821 gccacaaaga ccgcggccgt ctcgaagact tccccggcct gcgccgcccg ccgctgcgcg
     2881 cgcgcgccgg gagcgccgtg acgcagatgc actacgcgcg ccgcggccgt atcactcccg
     2941 agatggagtt cgtcgcgctg cgcgagaaca tgggccgcga gcgggcgctc cacgccatgc
     3001 gcgaacacac gcgcgacgac cgcagctcac tcttccacga gcacggcggc gagcgattcg
     3061 gcgcgcggat tcccgagttc gtcacgcccg agttcgtgcg cgatgaggtc gcgcgcgggc
     3121 gggccatcat ccccgccaac gtcaaccacc ccgagtccga gccgatgatc atcggccgca
     3181 acttcctcgt gaagatcaac gccaacatcg gcaactcggc cgtcgcctcc tccatcgacg
     3241 aggaggtcga aaagatgcgc tgggcgacgc gctggggcgc tgacaccgtg atggacctct
     3301 cgaccggcaa aaacattcac accacgcgcg agtggatcat ccgcaactcg cccgtcccca
     3361 tcggcaccgt gcccatctat caggcgctcg aaaaagtcgg cggccgcgcc gaagagctga
     3421 cgtgggaggt cttccgcgac acgctcgtcg agcaggccga gcagggcgtc gattacttca
     3481 ccatccacgc cggcgtgcgt ctgcccttcg ttccgctcac cgcgcgccgc acgacgggca
     3541 tcgtctcgcg cggcggggcg atcatggcca agtggtgtct ggcgcgccac gaggagagct
     3601 tcctctacac gcgcttccga gacatctgcg agatcatgcg cgcctacgac gtgtcgttct
     3661 ccctcggcga cggcctgcgc cccggctcta tcgccgacgc caacgacgag gcgcagttcg
     3721 ccgaactcga cacgctcggc gagctgacgc aggtggcttg ggagttagac tgtcaggtga
     3781 tgatcgaagg gccgggccac gtgccgatgc acctcgtcaa ggagaacatg gaccggcagc
     3841 tcgccgtctg cggcgaggcg cccttctaca cgctcgggcc tttgacgacc gatatcgcgc
     3901 ccggctacga ccacatcacc tcggccatcg gcgcggctat gatagggtgg ttcggcaccg
     3961 ccatgctctg ctacgtcacg cccaaagagc acctggggct gcccgaccgg caggacgtga
     4021 aagagggcgt catcgcctac aagatcgccg cccacgccgc cgacctcgcc aagggccacc
     4081 ccggcgcgca actgcgcgac aacgcgctct cgaaggcgcg cttcgagttc cgctgggaag
     4141 atcaattcaa cctcagcctc gaccccgtga ccgcgcgcga gttccatgac gaaacgctcc
     4201 cgcaagaagg cgccaagacc gcgcacttct gctccatgtg cggcccgcac ttctgctcga
     4261 tgaagatcac ggaagacgtc cgcaaatacg ccgccgaaca aggcatcgcc gaggaagagg
     4321 cgttgaagaa ggggatggaa gaaaagagca aagaattcgc cgccaaaggt tccgaacttt
     4381 acgccaaagc ataatcaatg ctggtagggc gcctac
//
LOCUS       DASVES010000197         5204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_974463, whole genome shotgun sequence.
ACCESSION   DASVES010000197 DASVES010000000
VERSION     DASVES010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974463"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VF240_07795"
     CDS             complement(<1..1001)
                     /locus_tag="VF240_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7175170.1"
                     /translation="MDLLSTLNEQQREAVTNTEGPLLILAGAGSGKTRVITVRIAHLI
                     AERDVPPANILAVTFTNKAAQEMRDRVTRLLQGERLQSAPLISTFHSLCVRILRREIE
                     KLEEGYTRSFTIYDQDDMIRVVKACIKDLGYNDEDKKLGPRVVHSAISHAKNSGLDPE
                     AYAARVEHTDERRAAIARVFRLYEERLVANNALDFDDLLIKAVRLLRKRQDVRDHYND
                     RFRYILVDEYQDTNALQFALINFLTERQQNICVVGDPDQSIYRWRGADISNILNFEEH
                     YRGARIIKLEQNYRSTQNILSTADAVIKRNTTRKEKTLWTASPAGSPIRYYQAFDAES
                     "
     gene            1162..1368
                     /locus_tag="VF240_07800"
     CDS             1162..1368
                     /locus_tag="VF240_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175171.1"
                     /translation="MTDASRRTESFGPDPVIEAYKRDVDRTLIRENLRLTVEQRFDAL
                     VRLQQFAAELRRAGEAASTTDEAR"
     gene            1365..1853
                     /locus_tag="VF240_07805"
     CDS             1365..1853
                     /locus_tag="VF240_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175172.1"
                     /translation="MTVDFRALISLLAGAGVEFIIVGGAAATAHGSARLTLDLDIVYR
                     RTPANISRLVRALAPHQPYPRGAPPGLPFTWDERTVSNGLNFTLTTALGALDLLAEIT
                     GGGGYDELLPHSDRLELYGVECLCLGLQRLVHVKRAAGRPKDFEAIAELEAIVEERRK
                     LG"
     gene            complement(1870..2427)
                     /locus_tag="VF240_07810"
     CDS             complement(1870..2427)
                     /locus_tag="VF240_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX7175173.1"
                     /translation="MQHANTLDAARSALVVIDMQESFRRPIPDFAEVAARIATVARAA
                     QLLRVSVIVTEQYPKGLGRTAEEIRAALPEDFEPIEKTAFSSCGAGEFVARLERTGAQ
                     QVIVCGIEAHICVNQTTHDLLERGFQVHLLTDCITARAPHNRQAGLDKMRHSGALPSS
                     SEMALFELLRDARHEQFKAIQKLIR"
     gene            3015..5153
                     /locus_tag="VF240_07815"
     CDS             3015..5153
                     /locus_tag="VF240_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7175174.1"
                     /translation="MKKYSRLLALPLALTMGGTPLFVAAQNNASVANTPTAPTAKRTP
                     KETKIHGETLVDDYFWMREKSNPEVISYLEAENAYTAALMKPTEAFQEALYKEMLGRI
                     KQTDQQVPFRENGYFYYTRTVEGKQYPIFARKKGATTAPEEITLDLNELSVGHKYTGL
                     GSYSVSDDGNLLAYSMDHIGHRDFILHLKDLRTGKELADSLGQVTFTFWAPDNKTLFY
                     GREDAAKRPYQVWRHTVGDPKEKDVLVAEEKDELFRLYAGRTRSRGYIFLQSSSATTN
                     ETSFIPADKPTESPRLILARQEGHEYEVDHAGDLFYILTNDKGRNFRLVTAPTSKPGK
                     DGWKEVIAHRPDVMLEYTDMFRDFYVLGERENGLQKLKLTDYKTGKSHYLEFPEPVYS
                     AGVSTNREFDTKKLRYAYNSFTTPASTYDYDVAARKSELLKRQEVLGDFDPARYQSER
                     IYATASDGTKIPISIVYKKGTKRDASSPLWLYAYGSYGSSQNVGFNSNRLSMLDRGVT
                     YAIAHIRGGGDLGKGWHDAGKMMVKKNTFTDFIAAADHLVGEKYTAKDRLVISGASAG
                     GLLMGAVLNMRPDLFKVAIVGVPFVDVINTMLDKSLPLTVQEFLEWGNPEKKEEYAYM
                     KSYSPYDNIAAKDYPAMLVRVSLNDSQVPYWEGTKYAAKLRAMKTDKNTLLLKANMGA
                     GHGGASGRYDALRDTAFDYAFILTQLGINQ"
BASE COUNT          987 a         1651 c         1652 g          914 t
ORIGIN      
        1 gactcggcgt cgaacgcctg atagtagcgg atgggcgagc cggcggggct ggccgtccag
       61 agggtcttct ccttgcgcgt ggtgttgcgt ttgatgacgg catcggcggt gctgaggatg
      121 ttctgtgtgg agcggtagtt ctgttcgagc ttgatgatgc gcgcgccgcg gtagtgctcc
      181 tcgaagttga ggatgttcga gatgtcggcc ccgcgccagc ggtagatgct ctggtcgggg
      241 tcgccgacga cgcagatgtt ctgctggcgc tcggtgagga agttgatgag ggcgaactgg
      301 agcgcgttcg tatcctgata ctcatcgacg aggatgtagc ggaagcggtc gttgtagtgg
      361 tcgcgcacgt cctgtcgctt gcggagcaga cgcacggcct tgatgagcag gtcgtcgaag
      421 tcgagcgcgt tgttggcgac gaggcgctcc tcgtagagac ggaagacgcg ggcgatggcg
      481 gcgcggcgct cgtcggtgtg ctcgacgcgg gcggcgtagg cttcggggtc gaggccgctg
      541 ttcttggcgt ggctgatggc ggaatggacg acgcgcggcc cgagcttttt gtcctcgtcg
      601 ttgtacccca aatcttttat gcacgccttg acgacacgta tcatgtcgtc ctgatcgtag
      661 atggtgaagg agcgcgtgta gccttcttcg agcttttcga tctcgcgccg caggatgcgg
      721 acgcagaggg aatggaaggt cgaaatcagc ggcgcgctct ggaggcgttc gccttggagc
      781 aggcgcgtga cgcggtcgcg catctcctgc gccgccttgt tcgtgaaggt gacggcgagg
      841 atgttggcgg gcggcacatc gcgctccgcg atgaggtgag cgatgcggac ggtgatgacg
      901 cgcgtcttgc ccgaccccgc gccggccaga atgaggagcg gcccctccgt gttcgtcacc
      961 gcttcccgtt gttgttcgtt gagtgttgag agcaagtcca tatgaaaatg aatggccgcc
     1021 gctgacgatt gtttagcacg ggctggtgac tggcgtgtgg ccgcagtgcg ttagttgttc
     1081 ggctgccgtc agagtttaat acatctgcgg ccggttagga catcggcggt gtataatgtc
     1141 ggcaaccgaa gggcgaagcc tatgactgat gcgagccggc ggactgagag cttcgggcct
     1201 gacccggtca tcgaggcata caagagggac gttgaccgca cgctgatccg cgagaacctg
     1261 cggctcacgg tcgagcagcg tttcgacgcg ctcgtgaggc tccagcagtt cgccgcggag
     1321 ctgcgacgcg ccggtgaggc cgcatccacc acggacgagg cgcgatgact gttgacttcc
     1381 gcgccctcat ctccctgctc gccggcgcgg gcgtcgagtt catcatcgtc ggcggcgccg
     1441 cggccacggc ccacggctcc gcgcgtctga ctctcgatct cgacatcgtc taccggcgaa
     1501 cgcccgcaaa catctcacgc ctcgtccgag cgctcgcccc acatcaacct taccctcgcg
     1561 gcgcgccgcc gggcctgccc ttcacatggg acgagcgcac cgtctcgaac ggcttgaact
     1621 tcacactcac aaccgcgctc ggcgcgctcg acctgctggc cgagatcacg ggcggcggcg
     1681 gttacgacga actgctccca cactccgaca ggctggagct ttacggcgtc gagtgtctct
     1741 gcctcggtct gcaacggctc gtccacgtca agcgcgcggc cggccgcccc aaagacttcg
     1801 aggccattgc cgagttggaa gcgatagttg aggagcggag aaagctcggc tgaagagacg
     1861 agttacggct cacctgataa gtttctgtat cgccttgaac tgttcgtgcc gcgcgtcgcg
     1921 cagcagctcg aagagggcca tctctgacga agaggggagc gcgccggagt gtcgcatctt
     1981 gtcgagaccg gcctgccggt tgtgcggggc gcgggcggtg atgcagtccg tgagcaggtg
     2041 tacctgaaat ccacgctcca gaaggtcgtg cgtggtctgg ttgacgcaga tgtgcgcctc
     2101 gatgccgcag acgatgacct gctgcgcgcc ggtgcgctca agccgggcga cgaactcgcc
     2161 cgcgccgcag gagctgaagg cggtcttctc gattggctcg aagtcttcgg gaagtgcggc
     2221 gcgaatctct tcggcggtgc ggccgagtcc cttcgggtat tgctcggtga cgatgacgct
     2281 cacacgcaaa agctgcgcgg cgcgggcgac cgtggcgatg cgcgcggcca cttccgcgaa
     2341 gtcggggatg ggccggcgga aagactcctg catgtcgatg acgacgagcg ccgagcgcgc
     2401 ggcgtcgagc gtgttggcgt gttgcatcgg gcctccggcg tgtgagactt atttaagcac
     2461 acggggtggg gaaacagtaa gcagtgagca gtaagcagtg agcagtaagc agtgagcggt
     2521 gagcatcaat gagcgccgga ggcgcggaag agtgtagcca cgggcgcaag cccgtggaat
     2581 agccgtaaat aaaaattgaa agccccggag gggcgaaaga atccgaagcc tgcctgaaac
     2641 gtggcttgaa gttctttcgc ccctccgggg ctttaacact ttaatcgaac tcgtacccac
     2701 ggctcacgcc gtgggctaca ttctgccgcg cctccggcgc tcgttgacgc ccgcggccta
     2761 ctgcttactg ctcactgctt actgctcact gctttccgcg gcttgccgcg cgcccccgcg
     2821 cgcatctaat atctgaaact ccgcgccggc ccgtgcgtaa aactttgcgc tcgcccgcaa
     2881 acgcttgggc cgcgacggcc ggcttcggaa attcgtccca gaatctgtat tcgatcacgc
     2941 gaaacccgac gggcgccctc gccggcgcct gccgaatttt cagaaaaact gttcaacttt
     3001 aacggaggtt cccgatgaag aaatattccc gcctgctcgc gctgccgctg gcgttgacga
     3061 tgggcggcac gccgctcttc gtcgcggcgc agaacaacgc gagcgtcgcc aacacgccga
     3121 ccgcgcccac cgccaaaagg acgccgaagg aaaccaaaat ccacggcgag acgctggtgg
     3181 acgactactt ctggatgcgc gagaagtcga acccggaagt gatctcctat ctggaggccg
     3241 agaacgctta cacggcggcg ctgatgaagc cgaccgaggc gttccaggag gcgctctaca
     3301 aggagatgct cggccgcatc aagcagaccg accagcaggt gcccttccgc gagaacggct
     3361 acttctatta cacgcgcacc gtcgagggca agcagtaccc gatcttcgcg cgcaagaagg
     3421 gggcgacgac cgcgcccgag gagatcacgc tcgacttaaa cgaactctcc gtcggccaca
     3481 agtacacggg cctcggcagc tattcggtct ccgacgacgg caacctcctc gcctactcga
     3541 tggatcacat cggccaccgc gacttcatcc tccacctcaa agacctccgc accggcaagg
     3601 aactcgccga ctcgctcgga caagtgacct tcaccttctg ggcgcccgac aacaagacgc
     3661 ttttctacgg ccgcgaggac gccgccaagc gcccctatca ggtctggcgt cacacggtcg
     3721 gcgaccccaa ggagaaggac gtgctcgtcg ccgaggagaa ggacgaactc ttccgcctct
     3781 acgccggccg cacgcgcagc cgcggctaca tcttcctcca gtcgtcgagc gcgaccacca
     3841 acgagaccag cttcatcccg gccgacaagc ccacggagtc gccccgcctc atcctcgcgc
     3901 gtcaggaggg gcacgagtac gaagtggatc acgcgggcga cctcttctac atcctgacga
     3961 acgacaaggg ccgcaacttc cgtctcgtca ccgcgcccac gtcgaagccg ggcaaggatg
     4021 gctggaagga agtcatcgcg catcgccccg acgtgatgct cgaatacaca gacatgttcc
     4081 gcgacttcta cgtcctcggc gagcgcgaga acggcctcca gaagctcaag ctcaccgact
     4141 acaagacggg caagtcgcac tacctcgaat tccccgagcc ggtctactcg gcgggcgtct
     4201 cgacgaaccg cgagttcgac acgaagaaac tgcgctacgc ctacaactct ttcaccactc
     4261 ccgcctccac ctacgactac gacgtggcgg cgcgcaagag cgagcttttg aagcggcagg
     4321 aagttctggg cgacttcgac ccggcccgct accagtccga gcgcatttac gcgacggcgt
     4381 ctgacggcac gaagattccc atctccatcg tctacaagaa gggaacgaag cgcgacgctt
     4441 cgtctccgct ctggctctac gcctacggct cctacggcag ctcgcagaac gtcggtttca
     4501 actccaaccg cctctcgatg ctcgaccgcg gcgtgaccta cgccatcgcc cacatccgcg
     4561 gcggcggcga cctcggcaag ggctggcacg acgccggcaa gatgatggtc aagaagaaca
     4621 ccttcaccga cttcatcgcg gcggccgacc acctcgtcgg cgagaagtac accgcgaagg
     4681 acaggctcgt catttcgggc gcgagcgcgg gcggcctcct gatgggcgcg gttttgaaca
     4741 tgcgccccga cctcttcaag gtggccatcg tcggcgtgcc cttcgtcgat gtcatcaaca
     4801 cgatgctcga caagagcctg ccgctcacgg tgcaggagtt tctggagtgg ggcaacccgg
     4861 agaagaagga agagtatgcc tacatgaagt cgtactcgcc ttatgacaac atcgccgcga
     4921 aagactatcc ggcgatgctc gtccgcgtct ccctcaacga cagtcaggtg ccctactggg
     4981 agggcacgaa gtacgcggcc aaactgcgcg cgatgaagac ggacaaaaac accctgctcc
     5041 tcaaggccaa catgggcgcc ggccacggcg gcgcgtcagg ccgctacgac gccctgcgcg
     5101 acacggcctt cgactacgcc ttcattctca cgcaactcgg catcaaccag taagccgcgc
     5161 gcgaggcgac aaggtaggcg gcaaagactg aaagcgcccc acga
//
LOCUS       DASVES010000198         6611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_537969, whole genome shotgun sequence.
ACCESSION   DASVES010000198 DASVES010000000
VERSION     DASVES010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537969"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF240_07820"
     CDS             <1..502
                     /locus_tag="VF240_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX7175175.1"
                     /translation="EMFVHRNVANLVVHTDLNCLSVMHYAVDVLGVKHIIVCGHYGCG
                     GVEAAYSNRQLGLIDNWLRHVQDVQHKHSALLAQFDDDGRRLEKLCELNVIEQVASAC
                     QTSVVQQAWGRAQELSVHGWVYSLKDGLLRDLNVCVTNRDEAQTVYETAISACCTPDP
                     KLDGTR"
     gene            551..1936
                     /locus_tag="VF240_07825"
     CDS             551..1936
                     /locus_tag="VF240_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7175176.1"
                     /translation="MFESLNIVIVFLLVFANGFFVASEFALVGVRRSRIATLANAGDK
                     RAKRLLGLVDNLNAYISATQLGITLASLALGWIGEPAIAHLLEVPLKGRVSEAVLHTI
                     ALTIAFSVITFLHIVLGELAPKTIALERAEKTALAIALPMQLFYKTFSYPIRLLDWAG
                     TRTVRLFGLHPSSEHASIYTEEELRQLIDISRRSGHLQEEEQKLINSVFDFSDAELRE
                     AMIPRTAVDAIPVTATLEETKKAFRTLGYSRLPVYRERLDDIVGVVFRKDLEPYLEQP
                     QPDGFKLEGLLHPPQFIPATARLGAVLKRMQSTRTHLAIVVDEHGGMEGIVTLEDVLE
                     EIVGEINDEYDEEVRSQILKEANGTYLLSGMLTVRDANRQLSLRLPEDGRYTTLAGFL
                     LAQAGYLLQEGETVEYEGMRFKVERVDQRRIRRIRFTPAPEDKQISPVALLSFVSTSL
                     MEPLQLAPILL"
     gene            2068..2358
                     /locus_tag="VF240_07830"
     CDS             2068..2358
                     /locus_tag="VF240_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175177.1"
                     /translation="MNHLKARLLAVVIILIFGGMMYYNWHQLGSEGRYSMKLAAFGPV
                     GVVGGLFLLLFPEKGGKPETGKDKVIALLVFGCGLIAGLYNWYLMDPGFFGR"
     gene            2500..3246
                     /locus_tag="VF240_07835"
     CDS             2500..3246
                     /locus_tag="VF240_07835"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX7175178.1"
                     /translation="MNDERSSREIVLRRALWSGVGVLAALGVVSAVGRAVSVASGGLS
                     YEQVVQLMPAQLEREAFEFDRWFVAHPALTFMHVIPGGIFLALAPFQFSTRIRSRYVR
                     FHRWSGRVLLLAALPVGVSGLMLGAVFPYGGPAAAAAVFVAGAVFLFALVRAFDAIRR
                     GDVARHREWMIRMFSLGLAIGTIRVVGLMLFPLNRIGFQDTHVGFHEAAGTVFWIGWA
                     STFAVAELWIRYTRPRPAAARVAPVRAAGA"
     gene            3313..3780
                     /locus_tag="VF240_07840"
     CDS             3313..3780
                     /locus_tag="VF240_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7175179.1"
                     /translation="MNRLSSTVAGGFINSFRDFAARVRELSGNLSEEQFWTKPYPYGN
                     SFGHLTLHLIGNLNYFIGAQIAGTGYVRDREREFTEGAPPKKEEVLRRLDEAVTMVSA
                     TLEAQADDDWSKDYEATAAADFIEDRFSIFLRCATHFHHHVGQMVYLEKEFSK"
     gene            complement(3809..4744)
                     /locus_tag="VF240_07845"
     CDS             complement(3809..4744)
                     /locus_tag="VF240_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175180.1"
                     /translation="MRTLNNSVLAILFAFALNASAHAQATPTPTPTPPPNTDIFVADL
                     SSAAGVPKLGAARRITTWEGYDNQPSFTPEGTDILYTSIRADNQADIYRYRLADNSTM
                     RLTETEEAEYSPTVTPDGKSFSVIRVERDREATQRLWKFPLAGAQTATLVLERFKPVG
                     YHVWADARTLGLFILGQPATLQVVDARTGVYGIVATNIGRSLQRVPGTQKITYVHKQG
                     TDDWHIKEYDTRTHAKRTLAKTLALSEDFAWTPDGTLLMAKGAKLYALRPGAAGQDWR
                     EVADFTSDGLSEITRLSVSPRGDRLALVARPRDTR"
     gene            complement(4820..5488)
                     /locus_tag="VF240_07850"
     CDS             complement(4820..5488)
                     /locus_tag="VF240_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175181.1"
                     /translation="MRLRHTLAAAPLLTACLLAGASSAHAQSAAQPEHALKVAAEIVE
                     QKYCVGAGGVDFLHTRLLLRYANAGSRRLIVYRGGNLFYQVVVYPAEEGPPRYELRTT
                     SARYATREPEPLDTPRPNVDFVSLAPGGEFRTEVSVTLPVTPAGVARVPNTVGVGKHS
                     LWLRVSTWYESKPLGDKLRERWARTGHLWTTPVSTAALRFDTERLRSTRACPGASATD
                     VKRD"
     gene            complement(5621..>6611)
                     /locus_tag="VF240_07855"
     CDS             complement(5621..>6611)
                     /locus_tag="VF240_07855"
                     /inference="COORDINATES: protein motif:HMM:NF028180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175182.1"
                     /translation="HAILYDTQTGKAWSPLSDMTARGGDGRSLVEVFDSSLSEQGVMG
                     FEYGYSVVARDALVAWEAQFGDFSNGAQVIIDQFIAPGVDKWQQPSRLVLLLPHGYEG
                     QGPEHSSARLERYLQLCAENNMQVCYPTTPAQYFHLLRRQVKQETARPLIVMTPKSLL
                     RLPAAVSSVAELTRGGFRPVIDDAEVQNREAVGRVVLCSGKVFYDLAAARKKSGDERV
                     AIVRLEQFYPFPEAALREIFGSYPAATQVVWAQEEAKNMGAWAFVEPRLAQMLPEGVR
                     PFYVGRAASASPATGSYAIHELEQRRLVDDALTTDAPFISGASTAQYAGQADS"
BASE COUNT         1175 a         1950 c         2160 g         1326 t
ORIGIN      
        1 cgagatgttc gtccaccgca acgtcgccaa cctcgtcgtc cacaccgacc tcaactgcct
       61 ctccgtgatg cactacgccg tggacgtgct cggcgtcaaa cacatcatcg tctgcggcca
      121 ctacggctgc ggcggagtag aagccgccta cagcaaccgc cagctcggcc tcatcgacaa
      181 ctggctgcgc cacgtgcagg acgtgcagca caagcactcc gcgctgctcg cgcagttcga
      241 cgacgacggg cgacgcctcg aaaagctgtg tgagctaaac gtcatcgagc aggtggccag
      301 cgcgtgccag acctcggtcg tccagcaggc gtgggggcgc gctcaggagc tttccgtaca
      361 cggctgggtc tacagcctca aggatgggct gctgcgcgac ctgaacgtct gcgtcaccaa
      421 ccgcgacgaa gcgcagacgg tctacgagac ggcaatcagt gcctgttgca cgcctgatcc
      481 gaagttagac ggcactcgct gataaatccg cttccttgcc tcgctactac ccaagcactt
      541 atcatcaagt atgtttgaaa gtctgaatat tgttatcgtt ttcctgctcg ttttcgctaa
      601 cggattcttt gtcgcctcag aatttgcgct cgtcggtgta cgccgctcac gcattgcgac
      661 ccttgccaat gccggtgata agcgggcgaa gagattgttg gggttggtgg acaatctaaa
      721 cgcctacatc tccgccaccc agcttggcat cactctggcc tcacttgcgc ttggctggat
      781 tggcgaaccg gcaatcgcgc atctgcttga agtcccgctt aaagggcgcg tctccgaagc
      841 cgtgctgcat accatcgctc tcaccattgc gttcagcgtc atcacttttc ttcatatcgt
      901 gctcggcgag cttgcgccta agaccatcgc tcttgaacgc gcggaaaaga ccgctctggc
      961 tatcgccttg ccgatgcagc ttttctacaa aacattttct tatccgatac ggcttctcga
     1021 ttgggcaggc acacgcactg tgcgattgtt cggccttcac ccttcgagcg aacacgcatc
     1081 catttatacg gaagaggaat tgcgtcagtt gatcgacatc tcgcgccgga gcggacacct
     1141 tcaagaagaa gaacaaaagc tcatcaatag cgtttttgat ttctccgacg ccgaacttcg
     1201 cgaagcgatg attccgcgca cggcggttga cgccatcccc gtgaccgcca cgttggaaga
     1261 aaccaaaaag gcttttcgca ctttgggcta ttcaaggctg cccgtttacc gtgagaggtt
     1321 agacgatatc gtcggcgtgg tattcagaaa ggatttagaa ccttacctcg aacagccgca
     1381 gccggacgga tttaaactgg agggcttgct tcacccgccg caatttattc cggcgacggc
     1441 gcggcttggc gcggtactga aacggatgca atcgacgcgc acacatttag ccattgtggt
     1501 agatgaacac ggcggcatgg aaggcatcgt cacgctcgaa gatgttctgg aagaaattgt
     1561 cggcgagatt aacgatgaat atgatgaaga agtgcgttcg caaatactga aagaagcaaa
     1621 cggcacatat ttattgagcg ggatgctgac tgtgcgcgat gccaaccgac agcttagtct
     1681 gcgtctgccg gaggatggcc gctacacaac gctcgccgga tttcttctcg ctcaagcagg
     1741 gtatttattg caggaagggg aaacggtcga atatgaaggg atgcgcttca aggtcgagcg
     1801 cgtagaccaa cgccgcatac gccggatacg ctttactcct gccccggaag acaagcaaat
     1861 atcacccgtc gcgttattat cattcgtcag tacctcgttg atggagccgc tacaacttgc
     1921 gccaatcctt ctttgatagg ggatgaatca tgagtttcag gcagttcgcg cggggcgggt
     1981 taatgtcggg cgtcaggttg ttccagtatt aaaatatgga ctgtatcggg gccattgttg
     2041 ggtgggcctc accgggagaa attgttgatg aatcatttga aggctcgtct cttggcggta
     2101 gtcatcatcc tgatctttgg cggcatgatg tattacaact ggcatcagtt gggcagtgag
     2161 ggccgctact cgatgaagct ggctgctttc gggcctgtcg gagtggtagg cggactcttc
     2221 cttctgttgt tcccggagaa gggcggaaag ccggagacgg ggaaggataa agtcattgcg
     2281 ctgctggtgt tcgggtgcgg ccttatcgca gggttatata actggtatct gatggacccg
     2341 ggtttcttcg gaaggtgaag ccggagtcgc cgtaacctga caacgccatg catccgaccg
     2401 catgccagcg ttcgtggtca tcgttaactt tagtagtaga gaagtcgagt gcgcggcggg
     2461 agatgccggg cgttagcccg cgcgaacgtg agtgcgaaga tgaatgacga gaggagcagt
     2521 agggaaattg tcttacggcg tgcgctgtgg tcgggcgtcg gcgtgctggc ggcgttgggc
     2581 gttgtctccg cggtcggcag ggccgtctcc gtggcgagcg gcggattgag ttacgagcag
     2641 gtcgtccaac tgatgccggc gcagttggag cgggaagcct ttgagtttga tcgctggttc
     2701 gtggctcacc cggcgctcac ttttatgcac gtcataccgg gcggcatctt tctcgccctc
     2761 gcacccttcc agttctcgac gcgcatcagg agtcgttacg tccggtttca tcgttggtcg
     2821 ggtcgcgtgc tgctgcttgc cgccctgccc gtcggagtgt cgggcctgat gctcggcgcg
     2881 gtgttcccgt atggaggccc ggcggcggcg gcggccgtct tcgtcgccgg cgctgttttc
     2941 ctcttcgcac tcgtccgggc ctttgacgcc attcgccgtg gcgacgtggc gcggcaccgg
     3001 gaatggatga tccgcatgtt ttcgctcggc ctcgccattg ggaccatcag ggtcgtcggt
     3061 ctcatgttgt tcccactcaa ccgaatcgga tttcaggaca ctcatgtcgg atttcacgaa
     3121 gcggcgggga cggtcttctg gatcggttgg gcgtccactt tcgccgtggc tgaattgtgg
     3181 atcaggtaca cgcgcccccg gcccgccgcc gcgcgggtcg cgcccgtgag ggccgcgggc
     3241 gcttagacgt gtcggcggtc aacgacggcc gtcaggtcgc gattgctgat ttgtgtcgga
     3301 ggtaaaaacg aagtgaacag gctatcaagc acggtcgccg gcgggtttat taattccttt
     3361 cgggactttg ccgcgcgtgt ccgcgagctg tctgggaatc tctcggagga gcagttctgg
     3421 acgaagccct acccttacgg aaacagcttc gggcatttga cgctccacct catcggcaat
     3481 ctcaactact tcatcggcgc gcagatcgcc ggcacggggt acgtgcgcga ccgggagcgc
     3541 gagttcacgg agggagcgcc gccgaagaag gaggaggtgc tgaggcgact cgatgaggcg
     3601 gtgacgatgg tgtcggccac gcttgaggcg caggccgacg acgactggtc gaaagactac
     3661 gaggcgacgg ccgccgccga tttcatcgaa gaccgcttca gcatttttct caggtgtgcg
     3721 acccacttcc atcaccacgt cgggcagatg gtctatctgg agaaagagtt ttcaaagtga
     3781 gccgatgcca ctctgacgcc gacgctgttt agcgcgtgtc gcgtgggcgg gcgacgaggg
     3841 cgaggcggtc gccgcgcggg ctgacgctga ggcgcgtaat ctccgagagg ccgtccgaag
     3901 tgaagtcggc cacctcgcgc cagtcctgtc cggcggcgcc gggccgcagg gcataaagct
     3961 tcgcgccctt cgccatgagg agcgtgccgt cgggcgtcca cgcaaagtct tcgctgagcg
     4021 cgagcgtctt cgcgagcgtc cgcttggcgt gcgtgcgcgt gtcgtactcc ttgatgtgcc
     4081 agtcgtccgt gccctgcttg tggacgtagg tgatcttctg cgtccccggc acgcgctgga
     4141 gcgagcggcc gatgttggtg gcgacgatgc cgtaaacgcc ggtgcgcgcg tccacgacct
     4201 gaagcgtggc gggctggccg aggataaaga ggccgagcgt gcgtgcgtcg gcccagacgt
     4261 gatagccgac cggcttgaaa cgctcaagca cgagcgtggc cgtctgagcg cccgcgagcg
     4321 ggaacttcca gagccgctgc gtggcttccc ggtcgcgctc gacgcggatg acggagaacg
     4381 acttgccgtc gggggtgacg gtcggcgagt actcggcctc ttccgtttcg gtgaggcgca
     4441 tggtcgagtt gtcggcaagg cggtaacgat agatgtctgc ctgattgtcg gcgcggatgg
     4501 aggtgtagag gatgtctgtg ccttcggggg tgaacgacgg ctggttgtcg tagccctccc
     4561 acgtcgtgat gcgccgcgcc gcgccgagtt ttggcacgcc ggcggccgat gacaggtcgg
     4621 cgacgaagat gtctgtgttc ggcggcggtg tcggcgtcgg ggtgggcgtc gcctgtgcgt
     4681 gcgccgaagc gttgagggca aacgcgaata agatcgccag gacagagtta tttagtgttc
     4741 gcatgatgac gttcggtggc ttgctctagc ttgaatgtgg ggcgcggtga atacgacgta
     4801 ccctaaattc aaaagcgatc taatcgcgct tcacatcggt cgccgacgcg cccggacaag
     4861 cgcgcgtcga gcgcagacgt tcggtgtcga acctgagcgc ggcggtggag accggcgtcg
     4921 tccagagatg ccccgtgcgg gcccagcgtt cgcgcagctt atcgccgagc ggcttcgact
     4981 cgtaccacgt cgagacgcgc agccacagcg agtgcttgcc gacgcccacg gtgttcggaa
     5041 ctctggcgac tccggccggc gtcaccggca gcgtcacgga gacctccgtc ctgaactccc
     5101 cgcccggcgc gagcgagacg aaatccacgt tcggccgcgg cgtgtccagc ggctccggct
     5161 cgcgcgtggc gtagcgcgcg ctcgtcgtcc tcagctcgta gcgcggcggc ccctcttccg
     5221 cggggtagac cacgacctga tagaaaaggt tgccgcccct gtagacgatc agccggcggc
     5281 tgccggcgtt cgcgtagcgc agcagcaggc gcgtgtgcag gaagtccacg ccgcccgcgc
     5341 ccacgcagta tttttgttcg acgatctcgg ccgcgacctt gagcgcgtgc tccggctgcg
     5401 cggccgactg tgcgtgcgcc gaagacgcgc ccgcgagtaa gcaggcggtg agtagcggcg
     5461 cggcggcgag cgtgtgtcgg agtcgcatga gcaactcaag gatgaaggcg gaaacagtac
     5521 cgcgagcgga agcctcgcgg gtctggacgg ccgatgccgg cctctcagga taggcgtact
     5581 cgctcgctac cgctcgcggt actgtttttc atccttgctc ttatgagtcg gcctgcccgg
     5641 cgtactgcgc cgtgctcgcg ccggagatga agggcgcatc ggtggtgagc gcgtcatcga
     5701 ctaagcggcg ctgttcgagt tcgtggatgg cgtaggagcc ggtggcgggg ctggccgagg
     5761 cggcgcggcc gacgtagaag gggcgcacgc cttcgggcaa catctgagcc agacgcggct
     5821 cgacgaacgc ccaagcgccc atgttcttcg cctcctcctg cgcccagacg acctgcgtgg
     5881 ccgccgggta tgagccaaag atttcgcgca gcgccgcctc ggggaacggg tagaactgtt
     5941 cgagtcgcac aatcgccacg cgctcgtcgc ctgatttttt gcgcgccgcc gcgaggtcgt
     6001 agaagacctt gcccgagcac aggacgacgc ggccgaccgc ctcgcggttt tgcacctccg
     6061 cgtcgtcgat gacggggcgg aagccgcccc gcgtcaactc tgcgaccgaa gagacggccg
     6121 ccggcagacg cagcaggctc ttcggcgtca tcacgatgag gggccgggcc gtctcctgtt
     6181 tcacctgccg gcgcaggagg tggaagtact gcgccggggt cgtcgggtag cagacctgca
     6241 tgttgttctc ggcgcagagc tggaggtaac gttcgagccg cgccgatgaa tgctccggcc
     6301 cctgcccctc gtagccgtgc ggcaggagca ggacgagacg cgagggctgc tgccacttgt
     6361 ccacgccggg cgcgatgaac tggtcgatga tcacctgcgc gccgttcgag aagtcgccga
     6421 actgcgcctc ccacgcgacg agcgcgtcgc gcgccacgac cgagtagccg tactcgaagc
     6481 ccatcacgcc ctgctcggag agcgagctgt cgaagacctc gacgagcgag cgcccgtcgc
     6541 caccacgcgc cgtcatgtcg gagagcggcg accacgcctt gccggtctgc gtgtcgtaga
     6601 ggatggcgtg a
//
LOCUS       DASVES010000199        19043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_806524, whole genome shotgun sequence.
ACCESSION   DASVES010000199 DASVES010000000
VERSION     DASVES010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19043
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806524"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..966)
                     /locus_tag="VF240_07860"
     CDS             complement(184..966)
                     /locus_tag="VF240_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175183.1"
                     /translation="MRSAYVALALACLVLALGASCAAGDAGNTNSQSNAAAPAPTTSA
                     DASPTPASEPALPPGSDARAYLERGIDAYKKNRDQEAVAAFEEAVRMEPDFAEAHYRL
                     GLAYSAVGRNDEAEKSFETAVKAYKKMSDEESENAEAQFYLGLTHVKLGKYEDAVKAF
                     KLAVKQAPEDSDKYYELGLAHSKLAQYDEAVRALEKAIDIDPDFFRAAEALTRAREGL
                     DRRKAFLKDLEKMRQKPKTKSNTNANTNASSAPAPTPRPSAP"
     gene            1219..2037
                     /locus_tag="VF240_07865"
     CDS             1219..2037
                     /locus_tag="VF240_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175184.1"
                     /translation="MEQDEGATSVNRSERTLISGAERASRSPRFDEGSIRRAQPAVPL
                     GEAGATRRGLRRPSPLVMLAALAAVVGLVAGAVGASVYMSRRASPREASAPAVETAQA
                     AAPPSATAASEEELFKPAASDLPDVSPPAETTPAAETGDTASADDEQATLREALGGWL
                     SATNARDIGRQMSFYDTRLDAFYLSRNTSARSVRAEKVRVLGSADLIDIQASGPAAIR
                     LGPDGRTAVMRFRKRYRVAGGGQDRRGEVVQELRWRKTGEGWKIVSERDVRVIN"
     gene            2204..3451
                     /locus_tag="VF240_07870"
     CDS             2204..3451
                     /locus_tag="VF240_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7175185.1"
                     /translation="MDEGRPTVPEAVDATAPGAVDSRPPDERARASNFFGLGRNVAAA
                     SAAVCLLGFGEELWKKFLPKYLEALGAGAGVVGLFGTARDFFDAVYQYPGGWIADRVG
                     RRRAFLTFIALASVGYVFYLLSPAWPYVFVGLAFSMAWASMASPAVFAVIGDALPRER
                     RAMGFTLQSILKRVPMTVAPLIGGALIARSGVVSGVRAGLLVTLGLAVVAVLVVLTIN
                     IPVSVGAPTNIRGVWRSFHAALKRLLVSDIIIRTCEGMADIFVVLYVTNVTGVGIAQY
                     GVLVAVQMATSILVYLPAAKMADRTGRKPFVIATFACFALFPLAVIAATDFSTLVVAF
                     VIGGLREIGEPSRKAMIVDFAEPHLRARSVGLYYLVRSLSITPAAAVGGLLWMIAPQT
                     PFIVAGIIGLAGVVVFALTVEER"
     gene            4044..6680
                     /locus_tag="VF240_07875"
     CDS             4044..6680
                     /locus_tag="VF240_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7175186.1"
                     /translation="MSLTPRRSELFASLALVMLLALPAFTSGASDGAPFRTATSLTAG
                     KDPVAIASADFNGDRAADLAVANSGSGDVSVFLGDGRGRFSAPTNFPAGRSPRAVVAA
                     DFDGDRTADLAVANYLSDSVSLLLGDGRGGFALAGQLSAGAGPHALASADFNGDGRAD
                     LAAANGKSNDVSLFLAGERGRFGAAVSVSAGVEPFSVTAADFNGDTRADLAVAATGSH
                     AVVILYGDGRGRFAPAVSYAAGTMPIAIASADFDGDKRFDLAAVNYGSNTLTLALGAR
                     VGGWLTAARAYAPVTPSTAGVTADFNGDRRPDLALVGNGSLSILLAGADGGYTPAPAV
                     PPLPDPEARATFVASADFNADGRADLAVTHTIVAGVAVLLGDGRGAFTKPVVYAVGDD
                     PEGVVVADFNADGRADLAVSNAASKNLSVLLGDGRGAFGKAQHFPLDAQPIALASGDF
                     NGDGHADLATLNTRQLNFSVLLGDGRGAFGAASNFPAARSARFLIVADLNGDGRLDLA
                     TPSDQLNVVSVYPGDGRGQFTTRTDYAAGARPFWLVARDFDGDGHADLATVNHRSGTL
                     SVLIADGRGGFRAPRVYAVGPTPTLVFAEDFDGDGPLDLLSMVYGSGSLSTVFGDGRG
                     NFSGGRNFAAPVKPYAAAVADFNADGRADVAVTGSEAGEVAVFLGGRGGDFSALQRIT
                     AGQNLRGVAAADFDGDGRADLAAVDSSTGKVSILLNAMNSAGVGTPLATPMATPAGGA
                     AAATGASDAGAGGGMSLLEGSWGGQGVSLTFDGAGVSIEFDCAHGRITQRLAPDERGR
                     FDVSGTHDEERGGASKAISGRDEDGKVSGGGAKAAERPARYAGRVEGETMTLTVTLAA
                     GRQIIGTFKLVRGAPARLRKCR"
     gene            7057..10149
                     /locus_tag="VF240_07880"
     CDS             7057..10149
                     /locus_tag="VF240_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7175187.1"
                     /translation="MNKRTPYQSPFKGSTRGIVSRVFTFALSAMMLVQGFALATPVSG
                     QSRARAVPPPAQQAMRQHTPQQRDITVGQPVNMRNLAASRTLAPSTAGRVELKPIHPP
                     MGNLQAGRRGVAINTSGLSAPQTASQAPVPSVTGQSPPVTTSFKSDDLRLGSIPPDTM
                     GAVGDTQIMTTTNEKIIVHDRKGVVLSNVTLDDFWATVLLGGNPPNTFDPKVLYDRFN
                     DRWIIVTSANAQTATSATLVAVSQTNDALGTWNRYAIDADAAAVAAGGKWADYPSIGF
                     NNNWISVQVNLFGFGTVSGYQGPAVYVINKAAAYANTLSTVSTFEGSFATCLASATQN
                     VELGCGFTMTPAITEDNTTSTLYLAEDWDSTAAQLRLSKLTGTPAAPVLTVGTQFPQS
                     ANSWRFDAPIIGTSGGYLLQRQQNIYALSGNRVTANDSRIQNAVLRNGTLWATHHVML
                     PTVPTAAGAPASTVANPDNHTAVQWWEIDPTIETGASQAPLQRGRIEDPTATNCHNGS
                     GGTVAACTPQGQFFAYANIAVNAAEDALIGYSRFSHLTLPKTAYSMRKGTDAVNTMRD
                     SQTFREGVGNYNIGAGSPFNIRFGDYSATMVDPLNDTDFWTIQEYADFQREIFGPGQF
                     AGVWSTWWALIKPTTTTTTAGNLIISEFRLRGPAGPRDEYVELYNPASTPLTVTTTDG
                     SDGWAVAFSANGTTITALAAIPNGTTIPARGHYLVENNVAAAGIAPYSLEGYPNVLGA
                     GIPAGSPVRPSDGDAMYTPDLADNGGVAIFRTGNAANMNAGARMDSVGFAGIAAGLFK
                     EGTGIPNITGTPTGQITFARRQQDGAPNPTPGRPQDTNDNAADFQFLNPVNETLGVQP
                     TLGAPGPENLDSPVYTGNVFIDRFDVAFGDTVAPNRVRKGCVVPPLAEECNPARSNLG
                     TMSFRRKVTNFTGAPVSRLRFRIKDITTLPVPPASGLADLRAITSVDIVVNGQTVKGT
                     TLEESTAPTQALGGGYNASWSVALPTPLAAGATINVQFLLGVQTNGNYRVFINAESLP
                     "
     gene            10374..11912
                     /locus_tag="VF240_07885"
     CDS             10374..11912
                     /locus_tag="VF240_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX7175188.1"
                     /translation="MNKQKSSDKRNAGSRAGRLLASFALVAALLACQQDAPQAVASPA
                     SDPAATTPAVQVSGIAAASGTQNSYADTVARVTPAVVTIRASRRSRDARQFPFMNDPS
                     FREFFGDRQRPQQQQPQERVERGLGSGVIVTADGFILTNHHVVDGAEDIRVELGDGRT
                     LQAKVIGSDQPSDLAVLKVEAAGLSVLPLGDSDKTRVGDVVLAVGNPLGVGQTVTSGI
                     ISAKGRQSGLSDGSFEDFIQTDAAINRGNSGGALVNTAGELIGINSQILSPSGGNIGI
                     GFSIPSNMARSVMEQLRASGKVRRGQLGVVAQKIDTDLASGLGISEARGLIVSFVRPG
                     SAAERAGIRRNDIVLALNGQSVNDPNTFRNRVASTAPGAEVTLTVLRDNREQEVRVSL
                     GELVAEGARGDREDGGEEREDTSAGAGKLGLTLRTLTPEVAQRLRLPAGTQGLLVAGV
                     DGAGPAAVAGITEGDVILEASRQSVRTLEDLQAALARAGARPLILLVGRGENQFFVTV
                     KPRQ"
     gene            complement(12025..14523)
                     /locus_tag="VF240_07890"
     CDS             complement(12025..14523)
                     /locus_tag="VF240_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7175189.1"
                     /translation="MDLVGSQRFPQWLIRASSAGGAALVLYSAYSLPFARFGLPLLLL
                     ALSSAVLSLKFSGHPPHDRRYIPVNEFFIILALLLFGNEAAVLAGALTVACTSLASSR
                     STGSVLLRFAITVGSTGLFLLVLHLGFGPVSALQQEGFSLSFVNAACVALLAQAVMHA
                     SVVAVGSPLPAGRSAVSNWFTCFFWRTLSVSAACVVACLATPVIFWAGVGNSLAVCAF
                     AALAGVAYSAYATNPSLCAARAKEQEKLERAAGGDMDRFRSAFDHAAIGMALVSSEGR
                     WLQVNRSLCEILGHSERELLSTDFLSITHPEDLGGALANIGHVLKGKSVTSQMEKRYI
                     HKNGHEVWVHWSVSRARDAYTKSVHLIFQIQDITDRKLAEQRLHYDAFHDVLTGLPNR
                     ALFMDHLKLAIARAQRHREQIFAVLYLDLDRFKIVNDSLGHTIGDQLLVGIAERLQKN
                     LRPGDTVARLGGDEFTILVEDIADEAEAIQIAERIQRELSLPFNLNGREVFTTISMGI
                     APSSTGYASAEDILRDADTAMYRAKTLGKSRYEIFDTAMHARALNLLQMETDMRRAVE
                     REEFFLHYQPIVALDTFALRGFEALVRWQHPERGFISPMDFIPVAEETGLIQQIGEWV
                     LREACREMQRWQTIFPNEHPLFISVNLSSKQFANNDLIERVASIMRETKIDPRTLKLE
                     ITESVVMENIDTATEMLKELRALGLQLSIDDFGTGYSSLSYLHRFPIDTLKIDRSFVT
                     RMAANNENTEIVRTIVVLAQNLGMDVVAEGVETNEQLTILRALGCEHGQGYFFSKPVN
                     KSGAEKIISETYEANFVSAASDIVKTPSKTVLVA"
     gene            complement(14996..16090)
                     /locus_tag="VF240_07895"
     CDS             complement(14996..16090)
                     /locus_tag="VF240_07895"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX7175190.1"
                     /translation="MTHTNTGKEGKGALRNDQDRLVFEEGFSFSGYERDPLYLNTGAK
                     KFTDISGVSGLDSITDGRAGVFADFDNDGDLDVFMTTIQGEAHLLFRNNVGQEGRHLR
                     VILEGDRATGRDAYGAVVRVKTSQGTLTKVKSGGSGFVSQHDPRLLFGLGGDERAQEV
                     EVTWPGGVAEKFAGETGAGSTVVLRKGAGRVDSVEVTRARLPDPLTRDEVFARGLRIA
                     VGQPLPDLVLKTAAGAPSSMRKHLRAGRRTLVNVWATWCVPCRREMPELERLRPGLAS
                     RGIDLLGLNVDTDPKADVRGFLRENRVGYPVLLGGVPAVEGLYATDELSVPMSILLDE
                     KGVVLEVIPGWSAKTQKQFEALAGEGERHK"
     gene            complement(16112..17881)
                     /locus_tag="VF240_07900"
     CDS             complement(16112..17881)
                     /locus_tag="VF240_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7175191.1"
                     /translation="MLAATSFDSLPALAQQAAPPPPQPQGQKVDRARLEVIEDLSESL
                     ANDLLELSVAVRDRDVGRTAEFFPARLTAAPFPSRPQAVKQSVKWIGTRGWEAAPAPP
                     VNVTNKTGALSFAVGADTKAERPTPRAEFLRAFSEFLSNFEEIEDVRFKVKDASFDAG
                     ASSVAGAKVPTAVVGSRGRARIAFYVIGRNREGRREWARGTFNTNVRYAENRRWQFET
                     FSLLGLDSMVAAVDLFSEVAAPAGLSRRLPAYGSPGNAGFVWHGAAAADFDRDGWLDL
                     FVTSKDRNLLYLNDRAGRFRDASAETGAAAVATGTAPLVLDYDNDGDADVFVSAVGQQ
                     ILLENRLVPDGKLAFVDSSLEAGVAVPAVGFSAVAGDVNADGRQDIYVTSYNRYGEIT
                     PDSWFRATNGTPNLLFINQGDGRFKEEAGKWGVDDRRWSYAAEFVDIDEDGKVDLYVA
                     NDFGEKAMYINRGDRFVDEARERGALDPGNGMGVSFGDYNNDGRLDLHATNMSSTAGN
                     RILGRLFPGEGPKDNVLKKLAAGNNLYENTGAGKFRDVTSEVGGFSGGWAYGGGFIDF
                     DNDGWEDLFTPNGFISGKSMKDT"
     gene            18180..18734
                     /locus_tag="VF240_07905"
     CDS             18180..18734
                     /locus_tag="VF240_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7175192.1"
                     /translation="MGRESRFVVQEHHASHLHWDFRLEMGGVLKSWAVPKGPSLDPSA
                     KRLAVEVPDHAVSYLTYTGQISEGHYGAGQVYRWDIGAYETDAGDALDGWRAGALKFR
                     LSGARLRGAWRLFRMKGRAERGRPLWLLQKLDDEFAEPGHAAELVGEPKARPAAAKKS
                     AAKKSGGGAKKSAKSGRSATSKNR"
BASE COUNT         3156 a         6420 c         6436 g         3031 t
ORIGIN      
        1 cttagagcga gtttagcctg agtgtaagga acgtagattc accgcggagg cgcggaagac
       61 gcggagttca cgccgcagag attcaaccct ctgcgtagtc tctgcgtcct gcggcgcctc
      121 cgcggtgaac taccacacca aacatccatg aatcgagttc gtgattcccc ggcgaggaag
      181 tgttcagggc gctgaggggc gcggggtcgg ggcgggcgca gaagaggcgt tcgtgttggc
      241 gttcgtgttg gacttcgtct tgggcttctg gcgcatcttc tcaaggtctt tgaggaatgc
      301 cttgcggcgg tcgagtcctt cgcgggcgcg tgtgagcgcc tcggcggcgc ggaagaagtc
      361 ggggtcgatg tcgatggctt tttcgagcgc gcgcacggcc tcatcatact gcgcgagctt
      421 gctgtgggcg aggccgagtt cgtaatactt gtcgctgtcc tccggcgcct gcttcaccgc
      481 cagcttgaac gccttgacgg catcctcata cttgcccagc ttgacgtgcg tcagccccag
      541 atagaactgc gcctccgcgt tctccgactc ttcgtcagac atcttcttgt acgccttgac
      601 ggccgtctcg aaactcttct cggcctcgtc gttgcggccg acggcggagt aggcgaggcc
      661 gagtcggtag tgggcctcgg cgaagtccgg ctccattcgg acggcctcct cgaaggcggc
      721 gaccgcttcc tgatcgcggt tcttcttgta ggcgtcgatg ccgcgctcca agtaagcgcg
      781 cgcgtcggag ccggggggaa gcgccggctc ggacgcgggt gtcggcgacg cgtcggcgga
      841 cgtggtgggc gcgggggcgg ccgcgttgct ttgagagttc gtgttaccgg cgtcgccggc
      901 ggcgcaggaa gcacccagcg ccagcacgag acacgccaga gcgagggcga cgtaggccga
      961 tctcatagtc ttatctgtca tggagaatta ttaggtcggg cgcaaaattt ctacgacaat
     1021 tcttccgccc agtcaacaac cggggagcgc gcgcggcagc gactcactca cgccgactgt
     1081 gaagagcgcg cggccgcgaa ggttgtatgt aagcgcgact tcggagtcgg ccgtgcgcgc
     1141 cgcgcgcgga atggctgttg cctgaaaaca tccgatgggc gaactgtgta cgggttaaat
     1201 tcattcggag agggtgagat ggagcaggac gagggcgcca cttcggtaaa tcgctcggag
     1261 cgaacactta tcagcggagc ggagcgggct tcgcgctcgc cgcgcttcga cgagggttcg
     1321 atacggcgcg cgcagccggc cgtcccgctg ggcgaggcgg gcgcgacgcg tcgcggcttg
     1381 cgccggccga gtccgctggt gatgctggcc gcgctcgccg ccgtcgtcgg gctagtcgcc
     1441 ggcgcggtcg gagcgtccgt gtacatgagt cggcgcgcgt cgccgcgcga ggcaagcgcg
     1501 ccggccgtcg agacggcgca ggccgccgcg ccgccgtccg ccacggccgc atccgaagag
     1561 gagttgttca agccggccgc gagtgacctg ccggacgtgt ctcctccggc ggagacgacc
     1621 ccggccgcgg agacgggcga cactgcctcg gccgacgacg agcaggccac gctgcgcgag
     1681 gcgctcggcg gatggctcag cgcgacgaac gcgcgcgaca tcggccgcca gatgagcttc
     1741 tacgacacgc ggctcgacgc cttctacctc tcgcgcaaca caagcgcgcg ctccgtgcgc
     1801 gccgagaagg tgcgcgtcct gggcagcgcc gacctcatcg acattcaggc cagcggcccc
     1861 gccgccatcc gtctcggccc cgatgggcgc acggccgtca tgcgctttcg caaacgctat
     1921 cgcgtcgccg gcggcggcca ggatcgtcgc ggcgaggtcg tgcaggagtt gcgctggcgc
     1981 aagaccggcg agggctggaa gatcgtcagc gagcgcgacg tgcgcgtgat caactgaggg
     2041 agctgttagc tgttagctat tagctgttaa cagtgtctcc cggccgcgaa gaaatacacc
     2101 tctggggcag ttttttcgcg ccccggcgtc gcggctaaag cttcggaaaa agaggggaaa
     2161 atgggcggcg cgggcgagcc ggcgcgggtt aagatgggtc gtgatggacg aaggcaggcc
     2221 cactgtgccg gaggcagttg atgcgaccgc gccgggggca gttgattccc ggccgccaga
     2281 cgagcgcgcc cgcgcgtcga actttttcgg tttgggccgc aacgtggcgg cggcctcggc
     2341 cgcggtctgt ctgctcggct tcggcgagga gctgtggaag aagtttctgc cgaagtacct
     2401 cgaagcactc ggcgctgggg cgggcgtcgt cggcctgttc ggcacggcga gagatttctt
     2461 cgacgccgtc taccagtatc ccggcgggtg gatcgccgac cgcgttggga ggcggcgcgc
     2521 cttcctcacc ttcatcgcgc tggcttcggt cggctacgtc ttctacctgc tcagccccgc
     2581 gtggccctac gtcttcgtcg ggctggcctt ctcgatggcg tgggccagca tggcgagtcc
     2641 ggccgtcttc gccgtcatcg gcgacgcgct gccgcgcgag cgccgcgcga tggggttcac
     2701 gctccagtcg attttgaagc gcgtgccgat gaccgtggcc cccttgatcg gcggcgcgct
     2761 gatcgcgcgc tccggggtcg tctcgggcgt gcgcgcgggg ctgctcgtga cgctcgggct
     2821 ggcggtcgtg gcggtgctcg tcgtgctgac gatcaacatc ccggtgagcg tcggcgcgcc
     2881 gacgaacatc cgcggcgtct ggcgctcctt ccacgcggcc ctcaagcgcc tgctcgtctc
     2941 cgacatcatc atcaggacgt gcgaggggat ggccgacatc ttcgtcgtcc tctacgtgac
     3001 gaacgtgacg ggcgtcggca tcgcgcaata cggcgtgctc gtcgcggtgc agatggcgac
     3061 ttcgattctg gtctacctgc cggcggcgaa gatggccgac cgcacggggc gcaagccctt
     3121 cgtcatcgcc accttcgcct gcttcgcgct cttcccgctc gcggtcatcg cggcgacgga
     3181 cttctcgacg ctcgtcgtcg ccttcgtcat cggcggcctg cgcgaaatcg gcgagccttc
     3241 gcgcaaggcg atgatcgtcg atttcgccga gccgcacctg cgggcgcgca gcgtgggcct
     3301 ctactatctc gtccgcagcc tctcgatcac gccggcggcg gccgtcggcg gactcctgtg
     3361 gatgatcgcg ccgcagacgc ctttcatcgt cgccggaata atcgggctgg ccggcgtcgt
     3421 cgtcttcgcg ctgacggtcg aagaacggta gtcaagccaa aagtaaaaag gctaaaggca
     3481 aaagttgaag agccacgtcg cgcgccgtca acgagccgct gtaagcggcg gaagaatgta
     3541 gcccacggcg tgagccgtgg gtcagcagtt aagaaaaaat aaggaagccc cggaggggcg
     3601 aaagaacctc gacgctggcg taggacgcac gggaagcttc tttcgcccct ccggggcttc
     3661 ttaacggtta tgcctgcgcg tccccacagc tcacgccgtg ggcaacactg ctgccgccgc
     3721 ttacagcggc tcattgacgc ccacgatcag cttctcaaat caagttcggg aatctgaacc
     3781 tccgcgcccg gcagacgcag caggaggttg tagctgccct cggggatggg cggcaggctg
     3841 aactcacata actcgttcaa cgcggcctca aagcgagccg cgtccgcacc gacgccctcg
     3901 atctccacac tgccgcccgc cttgcgctgg cgcgtcgcgc ggtgtgatga ttgaagccga
     3961 ttagtgcaac gcgcccccag accgtgccgt ttaattcatt cattcaacag gccgacacgt
     4021 gtggcctgaa aggtcttccc tcagtgtctc tgaccccgcg ccgaagcgag ttgtttgcgt
     4081 cgctcgcgct cgtgatgttg ttggctctgc ccgccttcac ctcgggcgcg tccgacggag
     4141 cgcccttccg aaccgccact agcctgacgg ctggcaaaga ccccgtcgcc atcgcctccg
     4201 ccgacttcaa cggcgaccgc gcggccgacc tcgccgtcgc caacagcggc tcgggcgacg
     4261 tgtcggtctt cctcggcgac ggccgcggcc ggttctccgc gcccacgaac ttccccgccg
     4321 gccgcagccc gcgcgccgtc gtcgccgccg acttcgacgg cgaccgcacg gccgacctcg
     4381 ccgtcgccaa ctatctctcc gacagcgtct cgctcctcct cggcgacggc cgcggcggct
     4441 tcgcgctcgc gggccagttg agcgccggcg cggggccgca cgcgctcgcc tccgccgact
     4501 tcaacggcga cgggcgcgcc gacctcgccg cggccaacgg caagtcgaac gacgtatccc
     4561 tctttctcgc cggcgagcgc ggccggttcg gcgcggccgt ctcggtgagc gccggcgtcg
     4621 agccgttctc cgtcacagcc gccgacttca acggcgatac gcgcgccgac ctcgccgtgg
     4681 ctgccacggg gtcgcacgcc gtcgtcatac tctatggcga cgggcgcggc cgtttcgcgc
     4741 cggccgtgag ctatgcggcc gggacgatgc ccatcgccat cgcctccgcc gacttcgatg
     4801 gcgacaagcg ctttgacctt gccgccgtca actacggctc caacaccctg acgctcgccc
     4861 tcggcgcgcg cgtcgggggc tggctcacgg cggcccgagc ttacgcgccc gtcacgccct
     4921 cgaccgccgg ggtgaccgct gacttcaacg gcgaccgccg gcccgacctc gccctcgtcg
     4981 gaaacggctc cctcagcatt ctgctcgccg gcgccgacgg cggctacacg cccgcccccg
     5041 cagtgccgcc cctgcccgac cccgaagccc gggcgacctt cgtcgcttcg gccgacttca
     5101 acgccgatgg ccgcgccgac ctcgccgtga cacacaccat cgtggccggc gtcgccgtcc
     5161 tgctcggcga cgggcgcggc gcgtttacga agccggtcgt ctacgcggtg ggcgacgacc
     5221 cggaaggcgt cgtcgtcgcc gacttcaacg ccgacggccg cgctgacctc gccgtctcga
     5281 acgccgcgtc gaaaaacctc tccgtcctgc tcggcgacgg gcgcggcgcg ttcggcaagg
     5341 cgcagcactt ccccctcgac gctcaaccca tcgcgctcgc gtccggcgac ttcaacggcg
     5401 acggccacgc cgacctcgcc accctcaaca cgcgccagct caacttctcc gttttgctcg
     5461 gcgacggccg tggcgcgttc ggggccgcct ccaacttccc ggccgcgcgc tcggcccgct
     5521 ttctcatcgt cgccgacctc aacggcgacg gccggcttga tctcgccacc ccgagcgacc
     5581 agctcaacgt cgtcagcgtc taccccggcg acggccgcgg ccaattcacc acgcgcacgg
     5641 actacgccgc cggcgcgcgg ccgttctggc tcgtcgcgcg cgacttcgac ggcgacggcc
     5701 acgccgacct cgcgaccgtc aaccacaggt cgggcaccct ctccgtcctc atcgctgacg
     5761 gccgcggagg cttccgcgcg ccgcgcgtct acgccgtggg gccgacgccc acgctcgtct
     5821 tcgccgaaga cttcgacggc gacggccccc tcgatctgct ctcgatggtt tatggctcgg
     5881 gcagcctctc gaccgtcttc ggcgacgggc gcggcaactt ctcgggcggg agaaatttcg
     5941 cggcccccgt caagccttac gccgcggccg tcgcagactt caacgccgac ggccgcgccg
     6001 acgtggccgt caccggctcc gaggccggcg aggtcgcagt ctttctcggc gggcgcggcg
     6061 gcgacttctc ggcgcttcag cgcatcacgg ccggccagaa cctgcgcggc gtcgcggcgg
     6121 ccgacttcga cggcgacggc cgcgccgacc tcgcggccgt cgattcttcc accggcaaag
     6181 tgtcgatcct gctcaacgcc atgaactcgg cgggcgtggg gacgccgctg gcgactccga
     6241 tggcgacgcc ggcgggcgga gcagcggcgg cgacgggcgc gagcgacgcc ggtgcgggag
     6301 gtggcatgtc tctgctggag ggttcctggg gcggtcaggg cgtcagcctg acgttcgacg
     6361 gcgcgggcgt ctcaatcgag tttgactgcg cgcacggccg catcacacag aggctcgcgc
     6421 cggacgagcg cggacggttc gacgtgagcg gcacacacga tgaagagcgg ggcggcgcga
     6481 gtaaagccat cagcggaagg gacgaggacg ggaaggtgag cgggggcggg gcgaaggccg
     6541 ccgagcgtcc ggcccgctac gccgggcgcg tcgagggcga gaccatgacg ctaacggtga
     6601 cgctcgccgc cggccgccag atcatcggca cgttcaaact cgtgcgcggc gcgccggcgc
     6661 gtctgcgaaa gtgccgttag cgcgtgcgcc cgcgcgccgg cgataggttt acaggcgcgc
     6721 tcaggtaagt cttgcggggc gtgccacatc tgacgtaata tgcaacctgc aaccgtccag
     6781 agcggacgcc gcggataaac gctcgcaagt atccacctcg cacttaactt gacagcgccg
     6841 agcgtagaag ggcagcgcaa gccgtaacgt ctgccgagca ctccgaccac gcaccttttg
     6901 cacccagatt tccagcactg aaatgagacc ccagcgcgtc gtctctgacg gacgagtccg
     6961 cgcctcgttg tgctgtttcc acccctcgcc aggcactcct cgacgctcga cgaactgccg
     7021 aagcgatcac cccatccatt taagggaaag aatccgatga acaaaagaac tccctaccag
     7081 tcgcccttca agggaagcac ccgcggcatt gtgtcgaggg tcttcacctt tgctctgagt
     7141 gcgatgatgc tcgtgcaggg cttcgccctc gccacgcccg tctccgggca gagcagggcg
     7201 cgggccgtgc cgccgcccgc gcagcaggcg atgaggcagc acacgccaca gcagcgcgac
     7261 atcaccgtcg gccaacccgt caacatgagg aatctggccg cgtcgcgcac gctcgccccc
     7321 tccaccgcgg gccgggtgga gttgaagccg atccatccgc cgatgggcaa cctgcaagcg
     7381 ggccgccgcg gcgtcgccat caacaccagc ggactgagcg cgccgcagac cgcgtcacag
     7441 gcccccgtcc cgtcggtgac cggacagtcg cccccggtga ccacgagctt caagtcggac
     7501 gacctgcgac tcggctccat cccgcccgac acgatgggcg cggtcggcga cacgcagatc
     7561 atgacgacga cgaacgaaaa gattatcgtc catgacagaa aaggcgtggt gctgagcaac
     7621 gtgacgctcg acgacttctg ggcgacggtg ctgctcggcg ggaatccgcc caacaccttc
     7681 gacccgaagg tgctctacga ccgcttcaac gaccgctgga tcatcgtcac gtcggcgaac
     7741 gcgcagacag caacctccgc cacgctcgtc gccgtctcgc agaccaacga cgcgctcggc
     7801 acgtggaatc gctacgccat cgacgccgac gcggcagccg tcgccgcggg cggcaagtgg
     7861 gccgactacc cgagcatcgg gttcaacaac aactggatca gcgttcaggt caacctgttc
     7921 ggcttcggca ccgtgagcgg ctatcaaggc ccggccgtct acgtcatcaa taaggccgcc
     7981 gcctacgcca acacgctctc gaccgtctcg accttcgagg gctccttcgc gacgtgcctt
     8041 gccagcgcga cccagaacgt cgagctgggc tgcggcttca cgatgacgcc ggccatcacc
     8101 gaggacaaca cgacctcgac cctttacctc gcggaagact gggattcgac cgccgcccag
     8161 ctccgcctga gcaagctcac ggggacgccc gccgcgcccg tgctcaccgt cggcacgcag
     8221 ttcccgcaat cggcgaactc gtggcgcttc gacgctccca tcatcggcac cagcggcggc
     8281 tatctgctcc aacgtcagca gaacatctac gcgctctccg gcaaccgcgt caccgccaac
     8341 gactcgcgca tccagaacgc ggtcttgcgc aacggcacgc tctgggcgac gcaccacgtg
     8401 atgcttccga ccgtgccgac ggcggcgggc gcgcccgctt cgaccgtggc gaaccccgac
     8461 aaccacaccg ccgtccagtg gtgggagatt gacccgacca tcgagacggg cgcgtcgcag
     8521 gcgccgcttc agcgcgggcg catcgaagac ccgaccgcca ccaactgtca caacggctcg
     8581 ggcggcacgg tcgcggcctg caccccgcag gggcagttct tcgcctacgc caatatcgcc
     8641 gtcaacgcgg ccgaagacgc gctcatcggc tactcgcgct tctcgcacct gacgctgccg
     8701 aagaccgcct actcgatgcg taagggcacg gacgcggtca acaccatgcg cgactcgcag
     8761 accttccgcg aaggcgtcgg caactacaac atcggcgccg gcagcccgtt caacatcagg
     8821 tttggcgact actccgcgac gatggttgac ccgctcaatg acaccgactt ctggacgatt
     8881 caggagtacg ccgacttcca gcgcgaaatc ttcgggccgg gtcagttcgc cggcgtctgg
     8941 tcaacgtggt gggcgctgat taagccgacg acgacgacga cgaccgcggg caacctcatc
     9001 atcagtgagt tccgcctgcg cgggcctgcg gggccgcgcg acgagtacgt cgaactctac
     9061 aacccggcca gcacgccttt gaccgtcacg accaccgacg gctcggacgg ctgggccgtc
     9121 gccttcagcg ccaacgggac gaccatcacg gcgctcgcgg ccatccccaa cggcacgacg
     9181 attccggccc gcggccacta cctcgttgag aacaacgtcg ccgccgccgg catcgcgccc
     9241 tacagcctcg aaggctaccc gaacgtcctc ggcgcaggga ttcctgccgg ctccccggtg
     9301 cggccttccg acggcgacgc catgtacacg cctgacctcg cggacaacgg cggcgtcgcc
     9361 atcttccgca cgggcaacgc ggcgaacatg aacgccggcg cccgcatgga ctcggtcggc
     9421 ttcgccggca tcgccgccgg tctcttcaag gaaggcacgg gcatcccgaa catcacgggc
     9481 acgccgacgg ggcagatcac ctttgcccgc cgtcagcagg acggagcacc caacccgacg
     9541 cccggaaggc cgcaggacac gaacgacaac gcggccgact tccagttcct caacccggtc
     9601 aacgagacgc tcggcgtgca gcccacgctc ggcgcgccgg ggcctgagaa cctcgacagc
     9661 cccgtctaca ccggcaacgt cttcatcgac cgcttcgacg tggcgttcgg tgacaccgtc
     9721 gcgcccaacc gggtccgcaa gggctgtgtt gtcccgccgc tggcggagga gtgcaacccg
     9781 gcgcgctcga atctcgggac gatgtcgttc cgtcggaagg tcacgaactt cacgggggcg
     9841 cccgtttcgc gcctgcggtt ccgcatcaag gacatcacga ccttgcccgt cccgccggcg
     9901 tctggcttgg ccgacctgcg ggcgatcacg tcggtggaca tcgtcgtgaa cggtcagacc
     9961 gtcaagggca ccacccttga ggagtcaacg gcgcccacgc aggcgctcgg cggcggctac
    10021 aacgcgagct ggtcggtggc actgcccacg ccgcttgcgg ccggcgccac catcaacgtg
    10081 cagttcctcc tcggcgtgca gacgaacggc aactaccgcg tcttcatcaa cgccgaatcg
    10141 ctgccgtaag ccgggcagaa cgcggccaac cccggcgcgc gccggcccga cttgaggccg
    10201 gcgcgcgccg cacaaactct gaaggcgcac gcggaacatt tcgcgtgcgc ctttttttcc
    10261 gccccgtcag agcgcgcacg ggtcgagcgc cacgcgccgg gcaaacttgc gccccccctc
    10321 gggaatctaa aaggggttac aaatttaccc gaagctttca ggagttttcc gagatgaaca
    10381 agcaaaaatc ttcagataag cgcaacgccg gctcccgcgc gggccgtctg ctcgcctcct
    10441 tcgccctcgt cgcggcgctc ctggcctgcc agcaggacgc cccgcaggcc gtcgcctcgc
    10501 ccgcctccga cccggcggcg acgacgcccg ccgtacaggt ctccggcatc gccgccgcct
    10561 cgggaacgca gaactcttac gccgacacgg tggcgcgcgt cacgccggcc gtcgtcacca
    10621 tccgcgcctc gcgccgctcg cgcgacgccc ggcagttccc gtttatgaac gacccgtcct
    10681 tccgcgagtt cttcggcgac cgccagcgcc cgcagcagca acagccgcag gagcgcgtcg
    10741 aacgcggcct cggctccggc gttatcgtca ccgccgacgg cttcatcctg accaaccacc
    10801 acgtcgttga cggcgccgaa gacatccgcg tcgaactcgg cgacggccgc acgcttcagg
    10861 ccaaagtcat cgggtcggat cagccgagcg acctcgcggt gctgaaggtc gaggccgcgg
    10921 gcctgagcgt gcttcccctt ggcgactcgg acaagacgcg cgtcggcgac gtggtcttag
    10981 ccgtcggcaa ccccttgggc gtcgggcaga ccgtgacctc cggcatcatc agtgcgaagg
    11041 gccggcagtc gggtctctcc gacggcagct ttgaagattt cattcaaacc gacgccgcca
    11101 tcaaccgcgg caactccggc ggcgcgctcg tcaacacggc gggcgaactc atcggcatca
    11161 actcgcagat cctctcgccc tcgggcggca acatcggcat cggcttctcc atcccctcga
    11221 acatggcccg cagcgtcatg gagcaactgc gcgcttccgg caaagtgcgg cgcgggcagc
    11281 tcggcgtcgt cgcgcagaag atcgacaccg acctcgcttc cggtctcgga atctccgagg
    11341 cgcggggcct catcgtctcc ttcgtccggc cgggcagcgc cgccgagcgc gccggcatcc
    11401 gtcgcaacga catcgtgctc gccttgaacg gccagagcgt caacgacccc aacaccttcc
    11461 gcaaccgcgt ggcctcgacc gcgccgggcg cggaggtgac gctcaccgtg ctccgcgaca
    11521 accgcgagca ggaggtgcgc gtctcgctcg gcgagctggt cgcggagggc gcgcggggcg
    11581 accgcgagga cgggggcgag gagcgcgaag acaccagcgc cggggccggc aagctcggcc
    11641 tcacactccg aacgctcacg cccgaggtgg cccagcgtct gcgtctgccc gcgggcacgc
    11701 aagggctgct cgtcgccggc gttgacggcg cggggccggc ggccgtcgcc ggcatcaccg
    11761 agggcgacgt gatcctcgaa gccagccgcc agagcgtccg cacgctcgaa gaccttcagg
    11821 cggccttggc ccgcgccggc gcgcgcccgc tgatcctgct cgtcggccgc ggcgagaatc
    11881 agttcttcgt caccgtcaag ccgcgccagt aagacaaaca gggccgaacg tcgaaaggcg
    11941 aaggggaagg ttacagacgg ttggtctgta accttcccct tcgccttccg gcgcgaagaa
    12001 tatcccgacg gccgagcggg cgcgtcaggc gacgagcacc gtcttcgacg gcgtcttgac
    12061 gatgtcggag gccgcggaga cgaagttcgc ctcgtaggtc tcggagatga tcttctcggc
    12121 gccgctcttg ttgacgggct ttgagaagaa gtaaccctgg ccgtgttcgc atcccagtgc
    12181 gcgcaggatg gtgagctgct cgttggtctc gacgccctcg gccaccacgt ccatgccgag
    12241 gttctgcgcg agcacgacga tggtgcggac gatctccgtg ttctcgttgt tggccgccat
    12301 gcgggtgacg aaggagcggt cgatcttgag ggtgtcgatg gggaagcggt ggaggtacga
    12361 gagactggag tagccggtgc cgaagtcgtc gatggagagc tgaaggccga gggcgcgcaa
    12421 ctccttgagc atctcggtgg ccgtgtcgat gttctccatg acgaccgact cggtgatctc
    12481 aagcttcaac gtgcgcgggt cgatcttcgt ctcgcgcatg atggaggcga cccgctcgat
    12541 gaggtcgttg ttggcgaact gcttgctgga gaggttgacg ctgatgaaga gcgggtgctc
    12601 gttggggaag atggtctgcc agcgttgcat ctcgcggcac gcctcgcgca agacccactc
    12661 gccgatctgc tggatgaggc ccgtctcctc ggcgacgggg atgaagtcca tcggcgagat
    12721 gaagccgcgc tcggggtgct gccagcggac taaagcctcg aagccgcgta gcgcgaacgt
    12781 gtcgagcgcg acgatgggct ggtagtggag gaagaactcc tcgcgctcga cggcgcggcg
    12841 catgtcggtc tccatctgga ggaggttgag cgcccgcgcg tgcatggccg tgtcgaatat
    12901 ctcgtagcgc gacttgccga gggtcttggc gcggtacatg gccgtgtcgg cgtcgcgcag
    12961 gatgtcctcg gcgctggcgt agccggtgga gctgggcgca atccccatgc tgatggtggt
    13021 gaagacctca cggccgttga ggttgaaggg cagagagagt tcgcgctgga tgcgctcggc
    13081 gatctggatg gcctcggcct cgtcggcgat gtcttcgacg aggatggtga actcgtcgcc
    13141 gccgaggcgg gcgaccgtgt cgccgggccg gaggttcttc tggaggcgct cggcgatgcc
    13201 gacgaggagc tggtcgccga tggtgtggcc cagagagtcg ttgacgattt tgaagcggtc
    13261 gaggtcgagg tagaggacgg cgaagatctg ctcgcggtgg cgctgggcgc gcgcaatcgc
    13321 gagcttcagg tgatccatga agagggcgcg gttcgggagg cccgtgagca cgtcgtggaa
    13381 ggcgtcgtag tggagacgct gctcggcgag cttgcggtcg gtgatgtcct gaatctggaa
    13441 gatgaggtgg acagacttgg tgtaagcgtc gcgcgcgcgc gagaccgacc agtggaccca
    13501 cacctcgtgg ccgttcttgt ggatgtagcg cttctccatc tgcgaggtga cggacttgcc
    13561 cttgagcacg tggccgatgt tggccagagc gccgccgagg tcttccgggt gcgtgatgga
    13621 gagaaagtcc gtggacagaa gttcgcgctc ggagtggccg aggatttcgc aaaggctgcg
    13681 gttgacctgc aaccagcgcc cttcggagga gacgagcgcc atgccgatgg cggcgtggtc
    13741 aaacgcgctg cggaagcgat ccatgtcgcc gcccgcggcg cgctccagtt tctcctgctc
    13801 tttagcgcgc gccgcgcaga gcgaggggtt cgtggcgtag gccgagtagg ccacgccggc
    13861 cagcgcggcg aaggcgcaga cggccagtga gttgccgacg cccgcccaga agatcacggg
    13921 cgtcgcgagg caggccacca cacaggcggc cgacaccgag agcgtgcgcc agaagaaaca
    13981 ggtgaaccag ttggagacgg ccgagcgccc ggccggcagg ggcgagccga cagcgacgac
    14041 cgacgcgtgc atcaccgcct gcgccaaaag cgccacgcac gcggcgttga cgaaggagag
    14101 cgagaagcct tcctgttgga gcgcggacac ggggccgaag cccaggtgga gtacgaggag
    14161 gaagaggccc gtcgagccga cggtgatggc gaacctgagc aacacggagc cggtgctccg
    14221 gctcgaagcg agcgaggtgc aggcgaccgt gagcgcgccg gcgagcacgg cggcctcgtt
    14281 gccgaagagc agcagcgcga ggatgatgaa aaactcgttg acggggatgt agcggcggtc
    14341 gtgcggcggg tggccggaga atttgaggct gaggacggcc gaggagagcg cgagcagcag
    14401 gaggggtaag ccgaagcgcg cgaagggcag gctgtaagcc gagtagagga cgagggcggc
    14461 gcctccggcc gaagacgcgc ggatgagcca ctgtgggaaa cgctggctgc ctactaagtc
    14521 catggggtta tggtttgtgc aaagaatgag ttggggaaac ctgctcgcga ggaagggggg
    14581 cagcctctcg gcgtccgttt tattaacaac tttggggcca ccagcgcgct agacgcgaaa
    14641 ccacgaaaaa tcaggaaaat acgcgctcgg cgaccctcag gggcgtgact cactcggtca
    14701 aaaagaggag gtcggtcacg gcaaaatgac atatgccgtc tacttcgccc cttaaccggc
    14761 tcgcgcgccg tcaatcagcc ccgtcagggg cggcagaaat gtagcccacg gcgcgagccg
    14821 tggggacaag acacgattaa gatttgaaag ccccggaggg gcgaaagaat tgcgacgttg
    14881 gcataggacg cacgtcgaag ttctttcgcc cctccggggc ttcattaatt ttgcccggcg
    14941 tgtccccacg gctcgcgccg tgggctacaa tcttcctccg cctccggcgg cttgctcact
    15001 tgtgcctttc tccctcgccc gccagcgcct cgaactgctt ctgcgtcttc gcggaccagc
    15061 cggggataac ttccagcacc actcccttct cgtccaacag tatcgacatc ggcaccgaca
    15121 actcgtcggt cgcgtacagc ccctcgaccg cggggacgcc gccgagcagg acggggtagc
    15181 cgacgcggtt ctcgcgcagg aagccgcgca cgtccgcctt ggggtctgta tccacgttga
    15241 ggccgaggag gtcgatgccc ctcgacgcaa gccccggccg caggcgttcg agttccggca
    15301 tctcgcggcg acagggcaca caccacgtcg cccacacgtt gacgagcgtc cgccggccgg
    15361 cgcgcagatg ctttcgcatg gatgacggcg cgccggccgc ggtcttcaac acgaggtcgg
    15421 ggagcggctg gccgaccgcg atccgcaagc cccgcgcaaa cacctcgtcg cgcgtgagcg
    15481 ggtcgggcag acgcgcgcgc gtgacctcga ccgaatcgac gcgccccgcg cccttgcgaa
    15541 gtacgacggt cgagccggcg cccgtctcgc ccgcgaactt ctccgccacg ccgccgggcc
    15601 acgtgacctc cacctcctga gcgcgctcgt cgccgccgag gccgaagagc aggcgcgggt
    15661 cgtgctgcga gacgaagccg gagccgccgc tcttgacctt cgtcagcgtg ccctgcgaag
    15721 tcttcactcg gacgaccgcg ccgtatgcgt cgcgccccgt cgcgcggtcg ccttcgagga
    15781 tgacgcgcag gtgccggccc tcctgcccga cgttgttgcg gaagagcagg tgcgcctcgc
    15841 cctgaatggt cgtcatgaaa acgtcgaggt cgccgtcgtt gtcgaagtcg gcgaagactc
    15901 cggcgcggcc gtcggtgatt gagtctagcc ctgagacgcc ggagatgtct gtgaacttct
    15961 tcgcgcccgt gttgagatag agcgggtcgc gctcgtagcc gctaaaggag aagccctcct
    16021 cgaagacgag tctgtcctga tcgttgcgga gcgcgccctt gccttctttg ccggtgttcg
    16081 tgtgcgtcac gacctgacgc cagaactggc ttcaagtgtc cttcatcgac ttgccggaga
    16141 tgaagccgtt gggtgtgaag aggtcttccc agccgtcgtt gtcgaagtcg atgaagccgc
    16201 cgccgtaggc ccagccgccg gagaagccgc cgacctccga ggtcacgtcg cggaacttgc
    16261 ccgcgccggt gttctcgtag aggttgttgc cggcggcgag ttttttgagc acgttgtctt
    16321 tcggcccctc gccggggaag agccggccga ggatgcggtt gccggccgtc gaagacatgt
    16381 tggtggcgtg gaggtcgagg cgtccgtcgt tgttgtagtc gccgaaggag acgcccatgc
    16441 cgttgccggg gtcgagcgcg ccgcgctcgc gcgcctcgtc aacgaagcgg tcgccgcggt
    16501 tgatgtacat ggccttctcg ccgaagtcgt tggcgacgta gaggtcaacc ttcccgtctt
    16561 catcgatgtc cacgaactcg gcggcgtaag accagcggcg gtcgtccacg ccccacttcc
    16621 cggcctcctc tttgaaacgg ccgtcgccct gattgatgaa gagcaggttg ggcgtgccgt
    16681 tggtggcgcg gaaccacgag tcgggcgtga tctcgccgta acggttgtag gaggtgacgt
    16741 agatgtcctg acggccgtcc gcgttcacgt cgccggcgac ggccgagaag ccgacggccg
    16801 ggacggcgac gcccgcttcc agagacgaat cgacgaaggc gagcttgccg tcggggacga
    16861 ggcgattctc cagaaggatt tgctggccga ccgcggagac gaacacgtcc gcgtctccat
    16921 cgttgtcgta gtcgaggacg agcggggcgg tgccggtcgc gacggcggcg gctcccgtct
    16981 cggcgctcgc gtcacggaag cggccagcgc ggtcgttgag gtaaagaaga ttgcggtctt
    17041 tggaagtgac gaagaggtcg agccagccgt cgcggtcgaa gtcggcggcg gccgcgccgt
    17101 gccagacgaa gccggcgttg ccgggtgagc cgtaagcggg caggcgacgc gagaggccgg
    17161 cgggcgcggc gacctcggag aagagatcga cggcggcgac catcgaatcg agaccgagga
    17221 gcgagaaggt ctcgaactgc cagcggcgat tctcggcgta gcgcacgttc gtgttgaagg
    17281 ttccgcgcgc ccactcgcgg cggccctcgc ggttgcggcc gatgacgtag aaggcgatac
    17341 gggcgcggcc gcgcgagccg acgaccgccg tcggcacctt cgcgccggcc acggagctag
    17401 cccccgcgtc gaagctcgcg tccttcacct tgaagcgcac gtcttcgatc tcctcgaagt
    17461 tcgaaaggaa ctccgagaag gcgcgcagga actccgcgcg cggcgtgggc cgctcggcct
    17521 ttgtgtcggc cccgacggcg aaggagaggg cgccggtctt gttggtgacg ttgaccggcg
    17581 gcgcgggggc ggcctcccag ccgcgcgtgc cgatccactt gacggactgc ttcacggctt
    17641 gcgggcgcga ggggaagggc gcggccgtca gacgcgcggg gaagaactcg gccgtgcgcc
    17701 ccacgtcgcg gtcgcgcacg gcgacggata gctcaagcaa atcgttggcg agcgactccg
    17761 agaggtcttc gatcacttcg agacgcgcgc ggtcaacctt ctgcccctgc ggctgcggcg
    17821 gaggcggcgc agcctgttgc gcgagcgcgg gcagcgagtc gaaggacgtt gccgcaagca
    17881 aaagcagagc gcatgcgtgg cgcagtcgtc taccgaaaaa cgcgcggcgg gcggcgtggg
    17941 agttgaacat gcctgttggt tctgctctct ttcgtctgtc gtgttgtaat cgtgcggccg
    18001 tgcgcttcgt ggagcgaggc gacgacgcag ggaattggag taacaaacta ccaccggggc
    18061 cgcgcgattt gcaaagagcc gtctccctgt tgcactttat ccatgacccc cgctgaattt
    18121 taaagacgcg cacggacgtt ctccgtggcg cggccgcttc cgggccgggc cgaggtgtga
    18181 tgggacgcga aagcagattc gtcgtgcagg agcaccacgc gagccacctc cactgggact
    18241 tccgtctgga gatgggcggc gtcttgaagt cgtgggccgt gccgaagggg ccgtcgctcg
    18301 acccttcggc gaagcgcctc gccgtcgagg tgcccgacca cgccgtctct tacctgacct
    18361 atacgggcca gatcagcgag ggccactacg gcgccgggca ggtctaccgc tgggacatcg
    18421 gcgcctacga aacggacgcc ggcgacgcgc tcgacggctg gcgcgcgggt gcgctgaagt
    18481 tccgcctgag cggcgctcgc ctgcgcggcg cttggcgact cttcaggatg aagggccgtg
    18541 ccgagcgcgg ccgcccgctg tggctcctgc aaaagctcga cgacgagttc gccgagccgg
    18601 gccacgcggc cgagttggtc ggagagccga aggcgcggcc ggcggcggcg aagaagtcag
    18661 ccgcgaagaa gtcgggcggc ggcgcgaaga agagtgcgaa gagtggcagg agcgcaacga
    18721 gtaagaatcg ttgaaggggg gacggccgaa gtccgcggcc cgcttctttg aaaacaatca
    18781 acatggatgg acaggaggga gaaaagttga ttggagattt cagatttcaa atcttcctat
    18841 cccgaccatc ctgtctattc atgttaataa ttctgtaagc gtgggcaggc acaagccctg
    18901 cccctacaaa tcaaacagga caagcgaggg taaatggcga caacaagcaa gggtgcgaag
    18961 aagaagtccg gcaagacgaa tcagtcgggc cggagtgtgg gcaaaaaggg cggcgcggtg
    19021 aaggccgcgg cgaagaagtc ggg
//
LOCUS       DASVES010000200         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_213325, whole genome shotgun sequence.
ACCESSION   DASVES010000200 DASVES010000000
VERSION     DASVES010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213325"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VF240_07910"
     CDS             complement(<1..593)
                     /locus_tag="VF240_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175193.1"
                     /translation="MSRVEKYTGALAKIFREAPDKHEANRRSRAVLEEMSGDAVFFSE
                     ALERHLRKPGSFNTRHYPVVSLDIELNPYYGLVANCWIPLPGRESHVSTKAIHHHGEM
                     LLSTATAFGPGYEHWTFTRPAEVEPASERYEMSLIERAAHPLHHVAFVDAYVAHLPFY
                     PPETTVTLALWSSRLPVTWKDRVKRVPLVQKNSAALKR"
     gene            complement(731..>2006)
                     /locus_tag="VF240_07915"
     CDS             complement(731..>2006)
                     /locus_tag="VF240_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175194.1"
                     /translation="AGREAVASLKSVSTLYLCYFGLREPLVQTQVLPYLRQLVAAGAV
                     VHLLTFEPLMRERWTARELEEERARLTGEGIRWHARAYHKRPSLPATLYDVQAGARFS
                     ARLLRRGEVNTLHARGHVAALMAALAKRAARGGGRMVFDIRGFMPEEYTDAGVWPEGG
                     ALYRGVKRVERFLFASSDAFIVLTEKAREILFPGRRDTDARGRPVEVIPCCVDFRRFA
                     AADATPKEALREEFGLTGRRVVVYAGSFGGWYMTDEMARFMAVAHRDDPTTYSMILTQ
                     SDASVVSEKLRALGVAEGDFFVGRVATGEVPRRMKAADIAVSFIRACYSKQSSSPTKI
                     AEYLAAGLPVVCNAGVGDVDAVIEGDGVGRILREFTDDAYRAALRAADEMRRDPATAE
                     HCRASARARFDLDTVGGAGYRRLYERLWGSDK"
BASE COUNT          329 a          656 c          707 g          314 t
ORIGIN      
        1 cgtttgagag cggcgctgtt cttctgtacg aggggcacgc gcttgacgcg gtctttccac
       61 gtcacgggca ggcggctcga ccagagcgcc agcgtgacgg tcgtctccgg cgggtagaac
      121 ggcaggtgcg cgacgtaggc gtcaacaaac gcgacgtggt ggagcgggtg cgcggcgcgc
      181 tcgatgaggc tcatctcgta gcgttcggag gccggctcga cctcggcggg gcgcgtgaac
      241 gtccagtgct cgtagccggg gccgaaggcc gtcgccgtcg agagcagcat ctcgccgtga
      301 tggtggatgg ccttcgtcga gacgtgcgac tcgcggcccg gcagcggaat ccagcagttg
      361 gcgacgaggc cgtagtaggg gttgagttcg atgtcgaggc tgacgacggg gtagtgccgc
      421 gtgttgaagg agccgggctt acgcaggtgc cgctccagcg cctcggagaa gaacacggca
      481 tcgcccgaca tctcttcgag cacggcgcgg ctgcgacggt tggcctcgtg cttgtcgggc
      541 gcctcccgaa aaatcttcgc gagcgccccg gtgtacttct ctactcgact cataaaatcc
      601 ccctgacgca agcgaacaga ttgccacagc cgccgcggca atatcaattg gggaagtgat
      661 aagtgatgag tgataagtga taagaaagag aagaagtcgc cccatcttcc ttcttatcac
      721 ttatcactca tcacttatca cttccccaaa ggcgctcgta gaggcggcgg tagccggcgc
      781 cgccgactgt gtcgaggtcg aagcgggcgc gggcgctcgc gcggcagtgt tcggccgtgg
      841 cggggtcgcg ccgcatctcg tcggccgcgc gcagggccgc gcggtaggcg tcgtccgtga
      901 actcgcgcag gatacggccg acgccgtcgc cttcgattac cgcgtccacg tcgcccacgc
      961 cggcgttgca gacgacgggc agccccgccg ccagatactc ggcgatcttc gtcggcgagg
     1021 acgactgttt cgagtagcag gcgcggatga aggagacggc gatgtcggcg gccttcatgc
     1081 gtcggggcac ttcgcccgtc gcgacgcggc cgacgaagaa gtcgccctcg gcgacgccga
     1141 gcgcgcgcag tttttcggag accacactcg cgtcgctctg cgtgaggatc atcgagtaag
     1201 tcgtcgggtc gtcgcggtgg gcgacggcca tgaagcgggc catctcgtcc gtcatgtacc
     1261 acccgccgaa cgagccggcg taaacgacga cgcgccggcc cgtgagaccg aactcttcgc
     1321 gcaacgcctc cttcggcgtc gcgtcggccg cggcgaagcg tcggaagtcc acgcagcacg
     1381 ggatgacctc gacggggcgt ccgcgcgcgt ccgtgtcgcg tcgccccgga aagagaatct
     1441 cgcgcgcctt ctccgtgagc acgatgaagg cgtcggatga tgcgaagagg aatcgctcga
     1501 cgcgcttgac gccgcggtag agcgcgccgc cctcgggcca cacgcccgcg tcggtgtact
     1561 cctcgggcat gaagccgcgg atgtcgaaga ccatgcggcc gccgccccgc gcggcgcgct
     1621 tggcgagcgc cgccatcagg gccgcgacgt ggccgcgcgc gtgcagcgtg ttgacctcgc
     1681 cacggcgcag cagacgcgca ctgaagcgcg cgcccgcctg cacgtcgtag agcgtggccg
     1741 gcagcgacgg ccgtttgtga taggcgcgcg cgtgccagcg gattccctca cccgtgagcc
     1801 gcgcgcgttc ttcttccagc tcacgcgccg tccaacgctc gcgcatcagc ggctcgaagg
     1861 tgagcaggtg aacgacggcg ccggccgcga cgagctgtcg caggtagggc agcacctgcg
     1921 tctgcacgag cggctcgcgc agaccgaagt aacagaggta gagcgtgctg acggacttca
     1981 gcgacgcgac ggcctcgcgg cccgcc
//
LOCUS       DASVES010000201        11528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_326890, whole genome shotgun sequence.
ACCESSION   DASVES010000201 DASVES010000000
VERSION     DASVES010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326890"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF240_07920"
     CDS             complement(<1..251)
                     /locus_tag="VF240_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX7175195.1"
                     /translation="MAKTQPARGMRDFLPADVRRREYVIRIIKEVYERYGFEPLETPA
                     VENIETLTGKYGEEGSQLIYKILKRGEHARTGEADLALR"
     gene            complement(328..840)
                     /locus_tag="VF240_07925"
     CDS             complement(328..840)
                     /locus_tag="VF240_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX7175196.1"
                     /translation="MIRPFRGKHPQIHPTAFIEESAQIIGDVVIGEGSSVWFNAVVRG
                     DVFYIRIGARTNVQDGSVIHVSNGTHATILEDEVTVGHNVTLHGCHVERGCLVGMGSI
                     VMDGVRVGERSLVAAGALLSPGTVVPPRSLVIGAPARVKRALTDEEVASLDLYWQNYI
                     EYTKQYKSDK"
     gene            complement(860..1387)
                     /locus_tag="VF240_07930"
     CDS             complement(860..1387)
                     /locus_tag="VF240_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175197.1"
                     /translation="MVEDVFHFKGHHLRATLLVLLLTVVLHAAACGGRKTGEGTTPAA
                     SAPTADAPALSDVEFAKDVFRRMAEGDPAVEPMLDWETLRMPGGDVAAEYNEIPGEAN
                     RAEFRKNFLKGFSTSFKASGGSAESAKNWREESKDGDRTQVATDSPTGNTLVFTVVRK
                     DGRQKISELVAREKK"
     gene            1630..1803
                     /locus_tag="VF240_07935"
     CDS             1630..1803
                     /locus_tag="VF240_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175198.1"
                     /translation="MPTSTPPPYFPQNALSPGDDKTRRKMRFTFYGCIVLAIAAIAGL
                     VVGAVYLVRWLWQ"
     gene            complement(2292..3602)
                     /locus_tag="VF240_07940"
     CDS             complement(2292..3602)
                     /locus_tag="VF240_07940"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7175199.1"
                     /translation="MSESGGTGARGRSLLRAFRHRNYRLYFAGQLVSLTGLWMQSVAQ
                     SWLVYRLTGSAVMLGLVGFAGQIPVFVLASVGGAVADRRDRRRILVVTQTCALALALS
                     LAALTLAGRVEVWHVFVFAAINGLVNAFDIPTRQAFVVDLVGRDDLMNAIALNSSMFN
                     GARVVGPAVAGLLVAWVGEGWCFLINGVSYVAVVAGLLSMRVEARAGARPEGSVLSNV
                     VEGFRFVARTAPVRALLMLLGLVSLMGMPYAVLMPIFADRILGGGVGGLGLLMGASGV
                     GALAGALSLAARQGLRGLGRWVALSSAGFGAALILFSQSRTFWLSALLLVPVGFAFMI
                     QMASSNTLVQAMAPDHLRGRVMAVYSMVFMGMAPFGALLAGTLANRVGAPTTLVIGGT
                     VCIAGAVAFGMRLPALRDEARQLIVAMQPAGGDPTEETGGVAVK"
     gene            4178..5956
                     /locus_tag="VF240_07945"
     CDS             4178..5956
                     /locus_tag="VF240_07945"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX7175200.1"
                     /translation="MPFNFDAIVIGTGFGGTVAATRLAAKGKKVLMLERGTWWITPAK
                     IGKAPQSALKKIPDWAKEQTPKHPVQYWTRPNHRQGLLDFFAAVRSGVNKDGLYQYSM
                     FKQADILTASGVGGGSLIYSNVTVRPVPEVLAGIGLGLTDADYAAAYKWMEDHRGRLN
                     HIVTKIPLPGRDVTNLTTDDYLYLDRARVLRDAAAAAGPKLGIQLPWKPLDLSLIEYD
                     PHRADGGDAKKVGTFCQREGRCILGCLPSARHTFSKTLYQKLLADPAKGVTLSPMAEV
                     RDIKQVPGGYEVNYRDHRDDNKKTVSAPMVFLCAGTLGTTEILLRSRDRGGLKITDKL
                     GTHFSTNGDFGAFVVGTTKPVHSSVGPINTCQVSARMGNLHIKVEDSAVPEMFAAITS
                     TTLNVLDNFAQREILKARMKFAFLQMAVPDLRDFFPHLPNTYDPESYQSEREMVSNIF
                     FFNVMGQDDASGKFTLSNDRLDLDWDKKIGETPVFQKIETLLKALAAEMGGRYTPFPL
                     WKGLGNKKLIVTHPLGGCPIAPTSFDGVVDELGRVFDGGKPKGSKDVLPGLFVVDGAA
                     IPGALATNPSLTIAAQALKAVTAALP"
     gene            5982..6944
                     /locus_tag="VF240_07950"
     CDS             5982..6944
                     /locus_tag="VF240_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175201.1"
                     /translation="MSNEGFFQLGLMDVSGRPVNESKVRVAFVRVADNKTILSAANLD
                     FPPSRRFAVPAFPQEKNLFCEVTPPRYRMRRSGVFTLTDGETIVRNLTLFRRPDEWAA
                     AFTAWDQLSSHFSPLKEVLRKSSAVKVRGGKLLGKFTEASYDGVNTKTEIFAKAALLN
                     LYTKLTDLKEPTGGKEPWFSFVREVVEIGKERFIAIVNPRMGDIVRAIKNDLGKFEGY
                     KNTPAQNHFGNIPAVFQVQKSKMFSLKSTEDHGNVQLTLAPGKDASGNDLLILDADID
                     ENGQLMAHLADLFKHKFTGGTHPFDIHEYLALDNPHRPLGYELV"
     gene            complement(7042..7632)
                     /gene="dcd"
                     /locus_tag="VF240_07955"
     CDS             complement(7042..7632)
                     /gene="dcd"
                     /locus_tag="VF240_07955"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX7175202.1"
                     /translation="MTVKSDRWIRRMAEEAGLIQPFNPTLVRETDGRRIISAGASSYG
                     YDMRLADDGFRVFSPITAREIDPKHFNDESLIEPPIRTADDGSRYYLMPPHSYALGVT
                     VETFNMPRNVTGICLGKSTYARAGLIVNTTPLEAGWKGRLVIELGNLADLPLRVYVGE
                     GIGQVLFFESDEDCSTSYEDRGGKYQGQTGLTYSRL"
     gene            complement(7739..8725)
                     /locus_tag="VF240_07960"
     CDS             complement(7739..8725)
                     /locus_tag="VF240_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175203.1"
                     /translation="MKHTQRKRPTRRALLARCAALTVASAATFGASALTHHAAAHSLA
                     AAPPHVQSGKRADSKPSARPTPAPRAAAPLKRTTARQETRRFAYGGRLTVYGAPQGSL
                     IVEAWERAEVEIAAEVELTGDTEEDLARLAAVNTFHLDDDVNHITVLTVGTHDRKYMK
                     RTAKDFPKRLLGQPWKIDYRVRVPVMTDLEIYSGRGALTIKGVEGAVRLIAGESDPTV
                     FTLAGGDVEATLQGGTVSLDVTARSWRGRGASLRLIRGDLNLALPPNFSGYVNAEVLR
                     TGSIENAYAGLVPRERTKPTERTLQGVAGSGGATLSLTVGDGRVRIKSAAAP"
     gene            complement(8831..9637)
                     /locus_tag="VF240_07965"
     CDS             complement(8831..9637)
                     /locus_tag="VF240_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7175204.1"
                     /translation="MGDAPHSLKRRARAGMARAARAFTRVSPALVLFSTLALFSPAEA
                     PAQKGFTKEYPATPNVRLFLINRSGTVEVIAWERAKVKVSARMESSSTRVVPQVSGDD
                     VTIDVERENREDKGDVNFTIYVPAYSTVDIQTKRGNIIVRNVRGSVVRARVSTEGDIE
                     LTGIRAQMVVAENIMGNILFDAELLDGGTYELKSTQGDIQLRINADAGFRLTATAPRT
                     RNIDVNGFAGRGQFEFFNDKRKVIGKVGNGGAKLNTTNTLGSISIAPRTR"
     gene            complement(9733..10473)
                     /locus_tag="VF240_07970"
     CDS             complement(9733..10473)
                     /locus_tag="VF240_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7175205.1"
                     /translation="MDCEKCQQLLSDFLEGTLKGGVRVALGAHLEGCVSCAEVRDELQ
                     MIVSAARESREHLYAPPDSRAMWLRISNTIEGERALRPAADPASSRPAPARASLWARL
                     FRSRWELTLPQMAAAVAAIAVSVSFITAVGVQGLRDVRTAQNENRAAALLPSRAALVE
                     DDSYPHPYLRQQQARISYLQQRVEQRKAAWNPRMRDSFDRSLGVIDQAVVGSLDELKR
                     NPHDEVSEEMLNSALRDKMELLREFSEQ"
     gene            complement(10696..11418)
                     /locus_tag="VF240_07975"
     CDS             complement(10696..11418)
                     /locus_tag="VF240_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7175206.1"
                     /translation="MELGDTSRLPARECALRSGPFAAGAFGAAGPARPEDLRDAPHAP
                     TNPDVAAFEIDKSKASDYELAQAAAAGDMRSFELMYERHNRRVFSLCLRMTQNVAEAE
                     DLAQEVFIQLYRKIGSFRGESQFTTWLHRLTVNQVLMHFRKRGVRLEQTTDEGEMPVQ
                     IVRGTEDPSKMPVVDRIALDNAVAALPPGYRSVFVLHDVEGHEHEEISRLLGCSVGTS
                     KSQLHKARMKLRMLLRQQNESP"
BASE COUNT         2003 a         3821 c         3710 g         1994 t
ORIGIN      
        1 tagcgcaggg cgagatcggc ctcgcccgtg cgcgcgtgct cgccgcgctt gaggattttg
       61 tagatgagct ggctgccctc ctcgccgtac ttccccgtca gcgtctcgat gttctcaacg
      121 gccggcgtct ccagcggctc gaacccgtaa cgctcgtaca cctccttgat gatgcggata
      181 acatactccc gccgccgcac gtctgccggc agaaagtcgc gcatcccccg cgccggttgt
      241 gtcttcgcca taaaaatagt gatgggtgat aagtgatgag tgatgagata agaagaagcg
      301 acgccttctc ttatcactta tcactcatca cttatcactt ttatattgct tcgtgtactc
      361 gatgtagttc tgccagtaga ggtcgagcga ggcgacctcc tcgtccgtca gcgctcgctt
      421 gacccgcgcg ggcgcgccga tgacgagcga gcgcggcggg acgaccgtgc cgggcgaaag
      481 aagcgcgccc gcggcgacga gcgaacgctc gccgactctc acgccgtcca tcacgattga
      541 ccccatgccg acgaggcaac cgcgttcgac gtggcagccg tgcagcgtga cgttgtgccc
      601 gaccgtcacc tcgtcttcga ggatggtggc gtgcgtgccg ttgctgacgt ggatgacgga
      661 gccgtcctga acgttggtgc gcgcgccgat gcggatgtag aacacgtcgc cgcgcaccac
      721 cgcgttgaac cagacggacg acccctcgcc gataaccacg tcgccgatga tctgcgcgct
      781 ctcctcgatg aaggccgtgg gatgtatctg cgggtgcttg ccgcggaaag gtctgatcat
      841 gttttagacg cgcgccccct cacttcttct cgcgggcgac gagttcggag attttctgcc
      901 ggccgtcttt gcggacgacc gtgaagacga gcgtgttccc cgttgggctg tcggtcgcaa
      961 cctgtgtccg gtcgccgtcc ttcgactcct cgcgccagtt cttcgcgctc tcggcgctgc
     1021 cgcccgaagc cttgaacgac gtggagaaac ccttgaggaa attcttgcga aactccgcgc
     1081 gattcgcctc gccgggtatc tcgttgtact cggccgccac gtcgccgccc ggcatcctga
     1141 gagtctccca atcgagcatc ggctcgaccg ccgggtcgcc ctcggccatg cgccgaaaca
     1201 cgtccttcgc aaactccaca tcgctcaagg ccggcgcgtc ggccgtcggg gccgacgccg
     1261 cgggcgttgt tccctcaccc gtcttcctcc ccccgcacgc cgccgcatgg aggacgacag
     1321 tcaacaggag cacgagaagc gtcgcccgca ggtgatgtcc tttgaagtgg aacacgtctt
     1381 cgaccatgtg attcacagcg cggcgccaga tgaaagcgaa aacggtaaca cgcgccgcgc
     1441 tacaggggca agcaacacga tgagcggcgc agatcgcgct cggccgcgtg cgtttccgca
     1501 ggattgaaag gtgcggcctg aggacgcggg gcgcgtgatt tgcgacgcgc acatgcgctg
     1561 agcgggcgcg cggtggtatc gtatcgccgg cccacaagag aaacaccacg accgaaggag
     1621 accgccgcct tgccgacctc aaccccgccg ccctacttcc cccagaacgc actctcgccc
     1681 ggcgacgaca agacgcgacg caagatgcgc ttcaccttct acggctgcat cgtcctcgcc
     1741 atcgccgcca tcgccggcct cgtcgtcggc gccgtctacc tcgtcaggtg gctctggcaa
     1801 tagagctgtt agcagttagc gattagctgt tagctgtcct tatttattaa ggtatgatct
     1861 attattcgag tgcggagtat ttagcttcgg aatgattatg tggcgtgatg ttctccttag
     1921 attctgcggg ttcctccttg cttttgtcgg ggcgtgtcta ttcctttacg ggttgtttat
     1981 ctgctggata ctccgcgatg gcttagggcc ggacagcgtg accagttccg ggtggcttgc
     2041 gctctcacgt ttcgtggagg agttctgggg cttcctgtgc gtatctatcc ttctgtgcgt
     2101 cgtcggcggg tatctctggc ggcgtccatc caacaacggc atgcacccga ccgcgaatca
     2161 gcggccctct catcgttagc ttgaatgttt ccgagtttga gtgcgcgggg ggtaatgccg
     2221 ggcgttagct gttagccaga agctcgcgga tttgcggtct ggctaacagc taacagctaa
     2281 tcgctaacag ctcacttcac ggccacgccg cccgtctctt ccgtcgggtc gccgccggcg
     2341 ggctgcatgg cgacgatgag ttgtcgggcc tcgtccctga gcgccggcaa gcgcattccg
     2401 aaggcgaccg ccccggcgat gcagaccgtg ccgccgatga caagcgtcgt cggcgcgccc
     2461 acgcggttcg ccagcgtccc cgccaggagc gccccgaagg gcgccatccc catgaagacc
     2521 atcgagtaga cggccatcac gcgcccgcgc aggtgatccg gcgccatcgc ctgtacgagc
     2581 gtgttcgacg aggccatctg aatcataaac gcgaagccca ccggcacgag caacaacgcc
     2641 gagagccaga acgtgcgcga ctgcgagaac aggatgagcg ccgcgccgaa gccggcggac
     2701 gacagcgcca cccagcggcc taagcctcgc aagccctgcc gcgccgccag actcagcgcg
     2761 ccggccagcg cgcccacccc cgacgccccc atcaaaagcc cgaggccccc cacgcccccc
     2821 ccgaggatgc ggtcggcgaa gatcggcatc aacaccgcgt aaggcatccc catcagactc
     2881 accagcccca acagcatgag cagggcgcgc acgggcgcgg tgcgcgcgac gaagcggaat
     2941 ccctcgacga cgttcgatag caccgacccc tccggccgcg cgcccgctcg tgcctccacg
     3001 cgcatcgaca gaagccccgc gacgaccgcc acgtagctca ccccgttgat gaggaagcac
     3061 caaccctcgc cgacccacgc caccagaagg cccgccacgg ccggcccgac aacgcgcgcc
     3121 ccgttgaaca tcgaagagtt cagcgcgatg gcgttcatca aatcgtcccg gcccacaagg
     3181 tctacgacga acgcctgccg cgtcgggatg tcgaaggcgt tgaccagccc gttgatggcg
     3241 gcgaagacga agacgtgcca cacctcgacg cggcccgcga gcgtgagtgc ggcgagcgag
     3301 agcgccagcg ccagcgcgca cgtctgcgtg acgacgagga tgcggcgtcg gtcgcgccgg
     3361 tcggccaccg ctcccccgac cgaagccagc acgaagaccg gaatctgccc cgcgaacccc
     3421 accagcccca gcatcacggc cgaccccgtc agccgataca ccagccagct ctgcgccacc
     3481 gactgcatcc acagccccgt caggctgacc aactgccccg cgaaatacag acggtaattc
     3541 cggtgcctga aagcgcgcaa cagcgaccgc ccgcgcgcgc ccgtgccccc cgattcgctc
     3601 atcgcggcaa tttacccgcg gaaagtgtcg cttctttcgc gcgacggtct taacttattg
     3661 ctatttgcgg aaggacgtga accgcagtac gcggaacagt ccgatgatcg tagacgtgga
     3721 ttgccggccc ttcttaaccg aagggccgtg gcggcccatc gtcggaaaaa ctcttgacag
     3781 cgttacgagt ccggcataga gtgcgccaat cgaaatggac gttagccggg gcacggcgcg
     3841 ctgccgcact gccccgactc aaagactctc aagtctgtcg cttttgaaag ccccgcttct
     3901 taacgcgccg cactcgccgc cgacgcgccg ccgctctcga aggcgcattc tcagcaacac
     3961 cttagcccat cgaacccgcg ggcgggacgc tatatcaagc tctggcggtc atgcggactc
     4021 cgtccgccga agcccttcga aaccctttat tgccgcgccg gactccttac ttatccgcgg
     4081 gcggcacaat ccccggcaat ttcagtcaac tcttttcacg accgccaccc gtcctcacgt
     4141 ctcggtcagg tgaggccatt atcccaggga ggtcttcatg ccgttcaact tcgacgccat
     4201 cgtcatcggc acgggcttcg gcggcaccgt cgccgccacc cgcctcgccg ccaagggtaa
     4261 aaaggtgctc atgctggagc gcggcacgtg gtggatcacg cccgccaaga tcggcaaggc
     4321 gccgcagagc gccctcaaga agatccccga ttgggccaag gagcagaccc cgaagcaccc
     4381 cgtccaatac tggacgcgcc cgaaccaccg gcagggcctc ctcgacttct tcgccgccgt
     4441 ccgcagcggc gtcaacaagg acggcctcta ccagtattcg atgttcaagc aggccgacat
     4501 cctcacggcg agcggggtgg gcggcggctc gctcatctac tcgaacgtca cggtgcgccc
     4561 cgttccggag gttttggccg gcatcggcct cggcctcacc gacgccgact acgcggcggc
     4621 ttacaagtgg atggaggatc accgcggccg cctcaatcac atcgtcacga agatcccgct
     4681 accggggcgc gacgtgacga acctgacgac ggacgactac ctctacctcg accgcgcgcg
     4741 cgtgctgcgc gacgcggccg ccgccgccgg gccgaagctc ggcatccagt tgccctggaa
     4801 gccgctcgac ctctccctca tcgagtacga cccgcaccgc gccgacggcg gcgacgccaa
     4861 gaaggtcggc accttctgcc aacgcgaggg ccggtgcatc ctggggtgtc tgccctcggc
     4921 ccgccacacc ttcagcaaga cgctctacca gaagctgctc gccgaccctg cgaagggcgt
     4981 gacgctctcg ccgatggccg aagttcggga catcaaacag gttcccggcg gctacgaggt
     5041 caactaccgc gaccaccgcg acgacaacaa gaagacggtc tccgccccga tggtcttcct
     5101 ctgcgccggc acgctcggca cgacggagat tcttttgcgc tcgcgcgacc gcggcggcct
     5161 caaaatcacc gacaagctcg gcacgcactt ctcgaccaac ggcgactttg gcgccttcgt
     5221 cgtcggcacc acgaagcccg tccacagttc tgtggggccg atcaacacct gtcaggtgag
     5281 cgccaggatg ggaaacctcc acatcaaggt ggaagactcg gccgtccccg aaatgttcgc
     5341 cgctataacc agcaccactc tcaacgtgct cgacaatttc gcgcaacgcg agatactgaa
     5401 ggcgcggatg aagttcgcct tcttgcagat ggccgtgccc gacctgcgcg acttcttccc
     5461 gcacctgccc aacacctacg acccggagag ctaccagagc gagcgggaga tggtctcgaa
     5521 catcttcttc ttcaacgtca tggggcagga cgacgcgagc ggcaagttca ccctgagcaa
     5581 cgaccggctc gatctggatt gggacaagaa gatcggcgag acgcccgtct tccaaaagat
     5641 tgagacactc ctcaaggcgc tcgccgccga gatgggcggc cgctacactc cgttcccgct
     5701 ctggaagggg ttgggcaata agaaactgat cgtgacccac cccctgggcg gctgtcccat
     5761 cgcgccgacg agcttcgacg gcgtggtgga cgaacttggc cgcgtcttcg acgggggcaa
     5821 gcccaaaggc tcgaaggacg tgctgccggg cctgttcgtc gtggacgggg cggccatccc
     5881 cggcgcgctc gccaccaacc cgtcgctgac gattgccgca caggcgttga aagcggtcac
     5941 cgcggctctg ccgtaagaga ccggccgaag gagtagtcga gatgagcaac gaaggattct
     6001 tccaactcgg cctgatggac gtaagcggca ggcccgtcaa cgagtcgaag gtgcgggtcg
     6061 ctttcgtccg cgtcgccgac aacaagacga tcctctcggc cgcgaacctc gacttcccgc
     6121 cttcacgcag gttcgccgtc ccggctttcc cacaggagaa gaacctgttc tgcgaggtaa
     6181 cgccgccgcg ctaccggatg cgccgctccg gcgtcttcac gctcaccgac ggcgagacga
     6241 tagtccgcaa cctgacgctc ttccgccgcc ccgacgagtg ggcagccgcc ttcaccgctt
     6301 gggatcagct ctccagccac ttctcgccgc tcaaagaggt gctcaggaaa tcttcggcgg
     6361 tgaaggtgcg cggcgggaaa ctactcggca agtttaccga ggcgtcctac gacggcgtca
     6421 acactaagac ggaaatcttc gccaaggccg cgctcctgaa tctctacacc aagctcacgg
     6481 acttgaaaga gccgacgggc ggtaaggagc catggttctc gttcgtccgc gaggtcgtcg
     6541 agatcgggaa ggagcggttc atcgccatcg tcaacccgcg catgggcgac atcgtccgcg
     6601 ccatcaagaa cgacctcggc aagttcgagg gctacaagaa cacgcccgcg cagaaccact
     6661 tcggcaacat cccggccgtc tttcaggtgc agaagtcgaa gatgttcagc ctcaaatcga
     6721 ccgaggatca cggcaacgtc caactgacgc tggcgccggg caaggacgcc tccggcaacg
     6781 acctcctcat cctcgacgcc gacatcgacg agaacggcca actgatggcc cacctcgccg
     6841 acctcttcaa gcacaaattc accggcggca cccacccctt cgacatccac gaatacctcg
     6901 ccctcgacaa cccccaccga cccctcggct atgagctagt ctagaggcga gagcggacga
     6961 cgcccagctc acctgaagac atggctgacg cctgtctaac gaccttcagc gcgcccgccc
     7021 cgtcgtcgaa aaatcacaaa atcacagacg ggaatacgtg agtcccgtct ggccctgata
     7081 cttgccgccg cggtcttcgt aggaggtgga gcagtcttcg tcggactcga agaagaggac
     7141 ttggccgatg ccttcgccca cgtagacgcg cagggggagg tcggcgaggt tgccgagttc
     7201 gatgacgagg cggcccttcc agccggcttc gagaggggtg gtgttgacga tgaggccggc
     7261 gcgggcgtag gtggatttcc cgagacagat gccggtgacg ttgcggggca tgttgaaggt
     7321 ctcgacggtg acgccgaggg cgtaggagtg cgggggcatg aggtagtagc gtgagccgtc
     7381 gtcagcggtg cggatgggcg gctcgatgag ggactcgtcg ttgaagtgct tggggtcgat
     7441 ctcgcgcgcc gtgatggggg agaagacgcg gaagccgtcg tcggcgagac gcatgtcgta
     7501 gccgtaagat gacgccccgg cggagatgat gcggcggcca tcggtctcgc ggacgagggt
     7561 ggggttgaag ggttgaatca gcccggcttc ttcggccatg cggcgaatcc agcggtcgga
     7621 tttgacggtc attgtttttg attccggagg aaaatgtttg agagagcggc agcccacgcg
     7681 cgcggcgggc cgacgcgcag catagcgggc cgcgcgtggc gaagtccaga cgggcgcgct
     7741 acggcgcggc ggcagacttg atgcggacgc ggccgtcgcc gacggtgagc gagagcgtcg
     7801 cgccgccgga gccggcgacg ccctgaaggg tgcgctcggt gggcttcgtg cgctcgcgcg
     7861 ggacgagtcc ggcgtaggcg ttctcgatgc tgcccgtgcg caagacctcg gcgttgacgt
     7921 agccgctgaa gttgggcggg agcgcgaggt tgaggtcgcc gcggatgagg cggaggctgg
     7981 cgccgcgtcc gcgccagctt cgggccgtca cgtcgaggct gaccgtgccg ccctgaagcg
     8041 tggcctccac gtcgccgccg gcgagcgtga agacggtcgg gtcgctctcg ccggcgatta
     8101 agcggacggc cccctcgacg cccttgatcg tgagcgcgcc gcgccctgag tagatttcga
     8161 ggtcggtcat cacgggcacg cggacgcggt agtcgatctt ccacggctgg cccaggaggc
     8221 gcttggggaa atctttcgcc gtgcgcttca tgtacttgcg gtcgtgcgtg ccgacggtga
     8281 ggacggtgat gtgattaacg tcgtcgtcga ggtggaaggt gttgacggcg gcgaggcgcg
     8341 cgaggtcttc ctcggtgtcg cctgtcaact cgacctcggc ggcgatctct acttcggcgc
     8401 gctcccacgc ctcgacgatg agcgagcctt gcggcgcgcc gtagacggtg aggcgaccgc
     8461 cgtaggcgaa gcggcgcgtc tcctgccgcg cggtggtgcg cttcaagggg gcggcggcgc
     8521 gaggggcggg cgtgggccgc gccgacggtt tcgagtcagc gcgcttgccg ctctgaacgt
     8581 gcggcggtgc ggcggccaga gaatgtgcgg cggcgtggtg cgtgagcgcg gacgctccga
     8641 aggtcgcggc ggaggccacc gttagggcag cgcagcgagc gaggagggcg cggcgcgtgg
     8701 ggcgctttct ctgtgtgtgt ttcaacggcg gctcctgtgt gaaagatgag gcgggcgccc
     8761 tcctcggtcg ttcggcctga gccgtcggga gagcgcccgc ctctggagat gtgtctcacg
     8821 ggtggtgagt ttagcgcgtt cgcggggcga tggaaatgga gccgagcgtg ttagtcgtgt
     8881 tgagcttggc gccgccgttg ccgaccttgc cgatgacctt gcgcttgtcg ttgaagaatt
     8941 cgaactgacc gcgcccggcg aaaccgttca catcgatgtt gcgcgtgcgc ggggcggtcg
     9001 ccgtcaggcg gaagccagcg tcggcgttga tgcggagttg gatgtcgccc tgcgtggact
     9061 tcagctcgta ggtgccgccg tcgaggagtt ccgcgtcgaa gaggatgttg cccatgatgt
     9121 tctcggcgac gaccatctgc gcgcggatgc cggtcagctc gatgtcgccc tcggtggaga
     9181 cgcgcgcgcg caccacggag ccgcgcacgt tgcggacgat gatgttgccg cgcttggtct
     9241 ggatgtccac ggtcgagtag gcggggacgt agatggtgaa gttcacgtcg cctttgtcct
     9301 cgcggttctc gcgctcgaca tcgatggtca cgtcgtcgcc cgagacctgc ggaacgacgc
     9361 gcgtcgagga cgactccatg cgggccgaga ccttgacctt ggcgcgctcc caggcgatga
     9421 cttccacggt gccggagcgg ttgatgagga agaggcggac gttcggcgtg gccggatact
     9481 ctttggtgaa gcccttctgc gcgggcgctt ccgccggcga gaagagggcg agagtagaga
     9541 acaggacgag ggcgggcgag acgcgtgtga acgcccgcgc ggcgcgcgcc atcccggcgc
     9601 gcgcacgtcg cttcaggctg tgcggtgcgt ctcccatcga actacggtgc gcgtgcgccc
     9661 gacggcgccg acacgcgccc tccctgtaaa cgatttcggc cccggcgggc gtgagaggcg
     9721 agaggcgctg actcactgct cgctaaactc ccgaagcagc tccatcttgt cgcgcagcgc
     9781 ggagttgagc atctcctcgg agacttcgtc gtgcgggttg cgcttcagct cgtcgagcga
     9841 gccgactacg gcctgatcga tcacacccag gctgcggtcg aacgagtcgc gcatgcgcgg
     9901 gttccatgcc gccttgcgct gctcgacgcg ttgctgcaag tagctgatgc gggcctgctg
     9961 ctgtcgcagg taggggtgcg gataagagtc gtcttcgacc agcgcggcgc gcgacggcaa
    10021 cagagccgcg gcccggttct cgttctgcgc cgtcctcacg tcccttaagc cctgcacgcc
    10081 gacggctgtg ataaacgaga cgctcaccgc gatggcggcc acggccgcgg ccatctgcgg
    10141 caacgtcagc tcccagcggc tacggaagag gcgcgcccag agactcgcgc gcgcgggcgc
    10201 cggacgcgat gacgcggggt cggccgcggg acggagcgcc ctctcgccct cgatggtgtt
    10261 gctgatgcgc agccacatgg cgcgcgagtc gggcggggcg tacaggtgct cgcggctctc
    10321 gcgcgcggcc gagacgatca tttgcagctc gtcgcgcact tcggcacaag acacgcaccc
    10381 ttcgaggtgc gcgccgagcg cgacgcggac gccgcccttc agcgtgcctt caagaaaatc
    10441 gctgagcagt tgttggcact tctcgcagtc catcttgctt aacctctgac gcggaactta
    10501 aaacctgcgg ccaatggcgc gtggccgacg cgtgttcttc gggggaggag atttcggcgc
    10561 gcgcgggccg cgtcttcgtt ttccaactac tttcgccggt ctctcgtgga ggccgcgcgc
    10621 gtccgttgtg acgttcgccg ctcgcgggat tgacggtgtc gcgaggcgcg actcccgaag
    10681 caacgttgtt ggttgctatg gcgactcgtt ctgttgcctc aaaagcatcc gcagcttcat
    10741 acgggccttg tgcagttgcg acttcgaggt gccgaccgag cagcccaaaa gccgcgagat
    10801 ttcctcgtgc tcgtgaccct caacgtcgtg gaggacgaag accgagcgat agccgggcgg
    10861 cagagcggcg acggcgttgt cgagcgcgat gcggtcaacg acaggcatct tcgacgggtc
    10921 ttccgtaccg cgcacgatct ggacgggcat ctcgccctcg tccgtggtct gctctaacct
    10981 gacgccgcgc ttgcggaagt gcatcaacac ctgattgacc gtcagccggt gcagccaggt
    11041 ggtgaactgc gactcgccgc ggaaactacc gatcttccgg tagagctgga tgaagacctc
    11101 ctgagcgagg tcttcggcct cggccacgtt ctgcgtcatc cgcagacaca ggctgaagac
    11161 gcggcggttg tgccgctcgt acatcagctc gaacgaacgc atgtcgccgg cggcggcggc
    11221 ctgcgccagc tcatagtccg acgcctttga tttgtcgatt tcgaatgccg ccacgtctgg
    11281 attggtcggg gcgtgcgggg cgtctctcaa gtcttcgggg cgcgccggcc ccgcagcgcc
    11341 gaaggcgccg gccgcgaacg gcccacttcg cagggcgcac tcacgcgccg gaagtcgtga
    11401 cgtgtcaccg agttccatcc cggggctgtg atgcggcgcc gcgggcgcgt cgttgcccct
    11461 tcccttccct aaatttagcg cgcgcgccaa agttcgtttc gtccgggaac atttcaactg
    11521 cgtgtgta
//
LOCUS       DASVES010000202        15770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_79283, whole genome shotgun sequence.
ACCESSION   DASVES010000202 DASVES010000000
VERSION     DASVES010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15770
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79283"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VF240_07980"
     CDS             complement(<1..1125)
                     /locus_tag="VF240_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7175207.1"
                     /translation="MSDYSPAARAYWFALVAAGVGVGAWAVGGLLALPTTGWAQLLVL
                     LSLIVISGARPLRIPGTKASVTAGDAFTFLGVIFLGVPAAVLLGAVDLLGSSVRTTRR
                     ATSWLAAPAVSAVTVFVAGHTFYAALVNHSGVTLNPLGTTAHVGLRALVLPLVLMALV
                     QYFVNGLAVATLIALKKRRPVWAYWSEGYLWTSWTFFAAAVAALVVYEAVMRFGVAYV
                     VLSVPVIWASYGTYRKYFEHVNEAEREAVEMGRIHLSTIEALATAIDAKDQTTHCHVR
                     RVQIYAARTGELLGLSAGEIKALKAGALLHDIGKLAVPDHILNKPGKLTPAEFERMKV
                     HTLVGAQILERVEFPYPVVPIVRHHHEQWNGRGYPDGLSGE"
     gene            complement(1734..2186)
                     /locus_tag="VF240_07985"
     CDS             complement(1734..2186)
                     /locus_tag="VF240_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175208.1"
                     /translation="MSLRLEAGADKFVTAVSHKPYDSLRDLIEALVALLEGRESAGVR
                     WNCEPDEFDLGFETRGGEVFIEVAHYPDHRRLTREVVFTTRQTESDLYRAFWRELRQL
                     RRRTVTDEFEQNWRRAFPEDELRRFTKTFRAHQRRRDADAAVSPRESV"
     gene            2347..3696
                     /locus_tag="VF240_07990"
     CDS             2347..3696
                     /locus_tag="VF240_07990"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7175209.1"
                     /translation="MAALTEGASVGYLELLRRNRDFRQLWLGQVVSQLGDWFNTIAVY
                     TLVLELTGSRRAVGLVLVARFLPTIVLGPLAGVVADRASRRTIMITSDVLRAVVVLGF
                     LFVRRPEHVWMIYALTLAQLALSTFFEPAKSAALPSIVSARELVPANAVSSVTWSVML
                     TVGAVAGGEVAGRFGADAAFLLDSVTYLASAALLLPIRLPKRAPRPKSKLTLAKALGI
                     TDTLEGIRYIGARRRVLALMLVKFAWCLGGGILSLLAVFGDVVFRLGDNAARGIGALY
                     AARGVGTAIGPLTARRVGGRGRHAMQKAIGISFIVGGVFYIAFGLATSFPVALAAVAL
                     AHMGGSALWIHSTVLLQRAVDEKFRGRVFAAEITLLTAALAASNYATGEALESFHVAP
                     RTVAIALGVFFIIPGVIWLATERWWDRGEEEAHAGADMAGEATAVAAGRDAPAAHGD"
     gene            3785..4576
                     /locus_tag="VF240_07995"
     CDS             3785..4576
                     /locus_tag="VF240_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7175210.1"
                     /translation="MDLGLRGRVALVAAASGGIGFAVARELAQEGARVFLCSRDEGRA
                     REAAERIAAETGAEVVGLRADVTSDEDVAAFVAAAQERAGGVDICVTNAGGPPKATFA
                     EADLDLYRLAFELNALSAVRLARLVVPGMRERGWGRLVNITSVSVKQPIEGLLLSNTI
                     RAGLTGWAKTLSNEVAADGVTVNNVAPGWTRTARQEEHEAANAARLGKSIEEMIEARA
                     SQVPARRMARPEEIAAAVAFLASERASYITGVTLQVDGGWVRGLF"
     gene            complement(4618..5160)
                     /locus_tag="VF240_08000"
     CDS             complement(4618..5160)
                     /locus_tag="VF240_08000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7175211.1"
                     /translation="MNKIIYLVRHGKATGQAADAPLTSEGIDQAEALAEFLLGVGVGQ
                     IVSSPFARALKSIKPLSIRLNIKIKLDERLIEAALSTIDYSDWPDKLRGTFSDFELSF
                     EGGESSQAATDRAIAAINDALLLDTNPIVVVTHGRLLTLILKHFDSKYGFEEWQHLTT
                     PDVFRIVIKENESQVDRIWQ"
     gene            5347..5994
                     /locus_tag="VF240_08005"
     CDS             5347..5994
                     /locus_tag="VF240_08005"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEX7175212.1"
                     /translation="MSYQIENIVEARHAGAEVAVADFGHEAWGRARPAHIARYWSGEA
                     APEGRHAEARVVWTDAALVVKFDCRQEEPLVVSSTPNLREKTIGLWDRDVCEIFLAPE
                     PSAPERYFEFEAAPTGEWIDLAIHWRAQGRETDWRYRSHMQTAALVADDSLSVTIRIP
                     FKSLGRSPRAGERWRANLFRCVGPDPVYRYLAWQPTHTEAPNFHVPESFGWLAFK"
     gene            complement(6036..6476)
                     /locus_tag="VF240_08010"
     CDS             complement(6036..6476)
                     /locus_tag="VF240_08010"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7175213.1"
                     /translation="MPERLRTLVNHLRRFVSDRRHAPRYPAHLPATVSHVSSAGNARH
                     HAGRPAPILSGHTRDLSATGISLVLPAIRIGDRYLMGEGRPVQITLELPTGAVQLRAV
                     PMRYERLGDEEGGERGYLVGAHIMEMDPDDMMRFGEFVRELAKG"
     gene            complement(6715..7518)
                     /locus_tag="VF240_08015"
     CDS             complement(6715..7518)
                     /locus_tag="VF240_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175214.1"
                     /translation="MPPPTLALVLCLASWAAPGLGMRTVAQQPAAPAAPPPPQQKAAP
                     GSPSFAVQEFYRLMRERRFREAFALSIFAPALEGLSAAEYEDLKPDFERQAGEVPEQV
                     ALTGEQMSGDDATVFMKLGTDEAPQVVPLPLIRERGAWLFGDREGQKLVKKQGKKYFF
                     EARMAAHQDDAENMLRRISTAQIIYSAQNGGLFGNLQQLVRSALIPADVLSTASTGYH
                     FHVETAPGGKSYTGGAEPARYGRTGRLSFYIDPSGLKKDDKGGKPLKGN"
     gene            complement(7781..9757)
                     /locus_tag="VF240_08020"
     CDS             complement(7781..9757)
                     /locus_tag="VF240_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7175215.1"
                     /translation="MKTKLLRAVSDRLFVALALASLACVCLAAWGIESPPAHAQAGAA
                     QQEGRRDKTPKAAVYGRVVYEGTNRPVRRARIMLLSPDGRGPEQLMGLTDARGEFRIK
                     GVSAGRYIAAVDVAGVISPISFAALESVRSDFLNFDEIVKNFDVFETTGKADKEVTVR
                     ARRGAALGGRVTYSDGEPAVGLTVHLMRKTGGRFAKTLTGFSIGSKTDDRGRFRIAGM
                     PPGEYVVAVSESAQHNEAGEGRSAFFMQGVAEVGGLFGQSLYMTFHPSATTEKAATVI
                     KVGAGDEREDVDITVAERDSRTVAGVVRGRGDRRPVARAMVAIFPRRPDAAQGAFELA
                     SLDEVFENGAQTDEEGRWVLKDIPDGSYTIFVKPPDAYEPTPQLFPPDGTAGISNANV
                     TVMEMNTNITVGNLNTAIVNDPPRRVKKYAPARVEVEVAGGDVSELAIELGEGGRVSG
                     TISFEGSRPLPDYVHVHTTRVGKGLSGAVEATASASASAYKGRFTLDGMGPGKYHLHA
                     SFYDRDGNTFHLKSITWRGKDLMREPLDIAEGAAVEGVSMVYTSDGATLKVNVRAASE
                     KTAGVGVHVLLVSADAARWSAHDAQQFHCSTRAGDSCEIKAAPGDYAVVVVLTPSSLV
                     GGFEEEVRKRAAGATRVSLRAGETKTVEAAAPEP"
     gene            complement(9886..12255)
                     /locus_tag="VF240_08025"
     CDS             complement(9886..12255)
                     /locus_tag="VF240_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX7175216.1"
                     /translation="MATDANTMKSVAVTNEYPDIVGARPENPLAASFRPYIPAHVEMP
                     ELTVRAVVVGTILGMVFGASSLYLVLKVGLTVSASIPVAVIAITLFRVFAGFGARNST
                     ILENNIVQTAGSAGESIAFGVGVTMPAIMILGFDLELTRVMLVAVLGGLLGILMMIPL
                     RRALIVQQHGILKYPEGTACAEVLKAGATEDSRAAADPLYRDAADERGGTNTGAKTIF
                     TGFGIGLLYTTLMKSFYLWKEVPEKIFGAPYRNGSVAVEASPALLGVGYIIGPRIAAI
                     MAAGGVLAYMVLIPMISFFGDFLGSAIIPPGTTPIGEMTPNGIRGAYVLYIGAGAVAA
                     GGIISLVRSLPTIWHGLRAGLADLRGGQAQTARSPRTDQDLSMKFVLAGIVALLAMIM
                     VLPQLNLQFNILGAILIIAFGFLFVTVSSRLTGEIGSSSNPISGMTVATLLLTCLVFL
                     FIGWTGPSYYVTALSIGAIVCIASSNGGTTSQDLKTGFLVGGTPYRQQIAILIGALAS
                     ALILGPILLRLNDASTVYVPAAQASLPATVRAPDPAQLTERETVPAAQGISDTQQYRV
                     WHKIDETGSPAGKYLVNEQGVPVYRVDPGINGNIRTRADGTPVDKYDAPKATLMSYII
                     KGILNRELPWGLVLLGVMIAVVLEMAGIPSLAFAVGVYLPLSSSSPILVGGLVRYLVD
                     KKLRQRLAHRNLTEDELVAEGDKSPGVLMASGYIAGGAIAGIIIAFMAGVLTDTNRRF
                     VEWAEANNPFHAGPWADVLALIPFGILVVLLYLVGREKLLAGRRVGDIS"
     gene            12454..14907
                     /locus_tag="VF240_08030"
     CDS             12454..14907
                     /locus_tag="VF240_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX7175217.1"
                     /translation="MFARALAPALASALALSSTLSLQARTQRSSAGEAARARSTAQGG
                     ARVRAAAAVPAPESVLGFRPGDDRKLAAWAQVVEYFRRLDAASPRVRFEELGKTTLGA
                     PFVVATISAPENLARLSEYKDIQRRLADPRLLGRRPDGRAASLVARGKAVVLITCGVH
                     STEVGSYLSSMLIAHRLASSDDAEVREILLNTIVLLVPSLNPDGVDIVKRWYDRTLGT
                     PHEGTSPPELYHPYVGHDNNRDWYAFTQAETRLTVDKIHNVWRPQIVHDIHQMGSTGA
                     RIFLPPYMQPVEPNVPVPVVEAYTELGRAVAAGLRAKGMKGVVTDAIYDAWTPARAYS
                     HYHGGARILSETASARIATPTTVKPEQLRAGSDDGYDPRKESANFPDPWPGGDWSLRD
                     ITGYMTSAAFELMKHAAQNREHWLRRFYEVGREAVRPRRRGELVGYKILGGPNTNALI
                     EILDRGGLEVRNVPVKTPRRGRGRRSAMDELGPDLFVPLAQPYGGFAKALLEKQNYPN
                     LRDPAGNPIQPYDVTAHTLPLLMGVEVEPVYEPIRTVALPTVRVGSAERDGVSFVMPP
                     AEPARPQAQARVGLYKSHVASMDEGWTRWVLEQEGFKYRSLLDAEARAGSLRAKYDAI
                     VLPDQSARAIFEGHRKGTMPDELTGGLGEEGVRALREFVERGGTLVCLNSASNFAIEH
                     LKLPVRNALAGLRRTDFFVPGSILRIVLDKSHPITEDMPQESVAWFEDSPAFEIAGDP
                     AALPRVKIVARYPQSGNPLLSGWLLGEQHLRGRAALVEVGMGEGRVILFGFRPQYRAQ
                     SLATYPLLFNAIAHGGSRP"
     gene            15007..15720
                     /locus_tag="VF240_08035"
     CDS             15007..15720
                     /locus_tag="VF240_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7175218.1"
                     /translation="MKRRTITLPALAFAALALLPLTVAAQQPAAQRQSVDLGAFTKEI
                     MLLRFDGTQQHLVMWLPYEFFVAAGTADGTMTRESMEKELGFLKSYLTLFVMSSIERP
                     DGASVYASDAEARKRAGLKLADGTEVRPLDVAPPKLTAIVAAMKALMAQQGGADRENM
                     HVLVFPAATSAGKTVVDVTRRDSLTLLLKADARFREATFTWRTPFDALTAVPDCPRCK
                     AGVSAKWTYCPYCGQKLPQ"
BASE COUNT         2579 a         5268 c         5264 g         2659 t
ORIGIN      
        1 ctcgcccgag aggccgtcgg ggtagccgcg gccgttccac tgctcgtggt gatggcggac
       61 gatgggcacc acggggtatg gaaactcgac gcgctccaga atctgcgcgc cgacgagcgt
      121 atgcaccttc atccgctcga actcggcggg cgtgagcttg cccggcttgt tgaggatgtg
      181 gtcggggacg gccagcttgc cgatgtcgtg gaggagcgcg ccggccttga gcgccttgat
      241 ctcgcccgcg gagaggccga ggagttcgcc cgtgcgcgcg gcgtaaatct gcacgcggcg
      301 gacgtggcag tgtgtcgtct ggtctttggc gtcgatggcc gtggcgagcg cctcgatggt
      361 cgagaggtgg atgcggccca tctcgacggc ctcgcgctcg gcctcgttga cgtgctcgaa
      421 atatttcctg taggtgccat agctcgccca gatgacggga acgctcagga cgacgtaggc
      481 cacgccgaag cgcatcaccg cctcgtagac gacgagcgcc gccacggccg cggcgaagaa
      541 cgtccacgaa gtccagaggt agccctcgct ccagtaggcc cagacgggcc ggcgcttctt
      601 gagcgcgatg agcgtcgcca cggccagccc gttgacgaag tactgaacga gcgccatgag
      661 gacgagcggc aggacgagcg cgcgcaggcc gacgtgagcg gtcgtgccca gagggttaag
      721 tgtgacgccc gaatggttaa cgagggcggc gtagaaggtg tggccggcaa cgaagacggt
      781 gacggccgag acggccggcg cggccagcca gctcgtcgcc cgccgcgtcg tccgcacgga
      841 gctgcccagc agatcgacgg cgccgagcag caccgccgcc ggcacgccga ggaagatgac
      901 gccgaggaag gtgaaggcgt cgcccgccgt cacgctcgcc ttcgtgccgg ggatacgcag
      961 ggggcgcgcg cccgagatga cgatgagact gaggagcacg agcagttgcg cccatccggt
     1021 cgtcggcagc gcgagcaggc cgccgactgc ccatgcgccg acgccgacgc ccgccgcgac
     1081 gagcgcgaac cagtaggcgc gcgccgccgg gctgtagtca gacatgtcga tgaatctcaa
     1141 agttctctcc ggccctgagt caattcgcat gattgattca ttgattcatc ggttcattga
     1201 ttcaattaaa gacaggcgct gcctttccat tgaatcaatg aaccgatgaa tcaatgaatc
     1261 aatccctttg aagggccgcc gccgaaagtg agagccggga gcaactcggc tttcgctttc
     1321 ggcggcggat tggggggaca agagcggcgg gccttactgt gacactcgcc cgccgtgctg
     1381 ggtgagaagt gatgcgcgcc gccggccccg ataagggcga ctcgctacgc gccggaagac
     1441 aaacccaatg aggggctgcc cttttcaaaa tggtgcgcgg ctttacggtt cccgtgcggt
     1501 ggccccgtgg agcgtccgaa gacgcccctt caagccgcgc gcagatagag ataacaagcg
     1561 ccgtgccgcg cgtgtcaatt ctcggttatg ttcgtttcgg ctattaaaaa tgcggggcgc
     1621 agcacgcgcg agggcagtgg cgctctagtc ttcaaagcgc gcgcgttcaa gctgacatgg
     1681 tcaggatgaa ggcccgtgtg aatgtgaccg aaggcgagtg gaggagtaga ggctcagacg
     1741 ctttcgcgcg gggagacggc ggcgtcggcg tcgcggcgtc tctggtgcgc cctgaaagtt
     1801 ttggtgaagc ggcgcagctc gtcttcgggg aaggcgcgcc gccagttctg ctcgaactcg
     1861 tcagtcaccg tgcgccttct tagctgccgc agctcgcgcc agaatgccct gtacaagtcc
     1921 gactccgtct gccgggttgt gaagacgact tcgcgcgtga ggcgtcggtg gtcgggatag
     1981 tgcgcgacct cgatgaagac ctcgccgccc cgcgtctcga aacccagatc gaactcatcc
     2041 ggctcgcagt tccacctgac gccggccgac tcgcgccctt cgagcagcgc gacgagcgcc
     2101 tcgatcaaat cgcgcagcga gtcgtatggc ttgtgcgaga cggccgtgac gaacttgtct
     2161 gctcccgcct ccagtcgcaa agacatccac ccgctctgcg ggctttcgaa gctgactcgt
     2221 agtttttcac tcatacgtag tgtcacagaa taaccgacgg cagcggccgg ttgacaagcg
     2281 ccggcgtgcc ggcccactct ctcgtcagca atttttattt tccgttccgg caataaactt
     2341 ttgaccgtgg ccgctctcac tgaaggcgcg agcgtcgggt atctggagct gctgcgccgc
     2401 aaccgcgact tccgacagct ctggctcggg caggtcgtca gccagctcgg cgactggttc
     2461 aacaccatcg ccgtctacac gctcgtcctc gaactgacgg ggtcgcgccg cgccgtcggc
     2521 ctcgtgctcg tcgcgcgctt tctaccgacc atcgtcctcg ggccgctcgc cggcgtcgtc
     2581 gccgaccgcg cgagccgccg cacgattatg attacgagcg acgtgctgcg cgcggtcgtc
     2641 gtgctcggct tcctgttcgt ccgccgcccc gagcacgtct ggatgatcta cgcgctgacg
     2701 ctcgctcagc tcgcgctctc gaccttcttc gagccggcca aatcggccgc gctgccatca
     2761 atcgtctcgg cgcgcgaact cgtgccggcc aacgccgtct cctccgtcac atggtctgtg
     2821 atgctcaccg tcggcgcggt ggcgggcggc gaggtcgccg ggcgcttcgg cgccgacgcg
     2881 gccttcctgc tcgactccgt cacctatctc gcctcggccg ccctcctcct ccccatacgt
     2941 ctgccgaagc gagcgccccg cccgaagtcg aagctcacgc tcgccaaggc gctcgggatt
     3001 accgacacac tcgaaggcat ccgctacatc ggggcgcgcc ggcgcgtgct cgcgttgatg
     3061 ctggtcaagt tcgcgtggtg tctgggcggg ggcattctgt cgctcttggc cgtcttcggc
     3121 gacgtggtgt tccgcctcgg cgacaacgcg gcgcgcggca tcggggcgct ctacgccgca
     3181 cgcggcgtgg gcacggccat cgggccgctc acggcgcgtc gcgtcggcgg ccgcggccgg
     3241 cacgcgatgc agaaggccat cggcatctcg tttatcgtcg gcggcgtctt ctacatcgcc
     3301 ttcggtctgg cgacgagctt ccccgtggcg ctcgcggccg tcgcgctcgc gcacatgggc
     3361 gggagcgcgt tgtggattca ctcgacggtg ctcttgcagc gggcggtgga cgaaaagttc
     3421 cgcggccgtg tcttcgccgc cgagataacg ctgctcaccg cggcgctcgc cgcctccaac
     3481 tacgcgaccg gcgaggctct cgaaagcttc cacgtcgcgc cgcgcacggt cgccatcgcc
     3541 ctcggcgttt tcttcatcat ccccggcgtc atctggctcg cgaccgagcg ttggtgggat
     3601 cgcggcgagg aagaggcaca cgcgggcgca gacatggcgg gcgaagcgac ggcggtcgcc
     3661 gccggacgcg acgcgcccgc cgctcacggc gactgacagg ggagcgtcca atcaattatc
     3721 atcgccactc gttcgtcgaa gcgtgtgggc gcggggcagt cagcggcgag aggagagagt
     3781 tgagatggat ttaggcttga ggggccgcgt ggcactggtc gccgcggcga gcggaggcat
     3841 cggcttcgcc gtcgcgcggg agttggcgca ggaaggcgcg cgcgtcttcc tctgctcgcg
     3901 cgacgagggg agggcgcggg aggcggccga gcggatcgcg gccgagacgg gcgcggaagt
     3961 cgtcggctta cgtgcggacg tgacgagcga cgaggacgtg gccgcgttcg tcgccgccgc
     4021 gcaggagcgt gccggcggcg tggacatctg cgtgacgaac gcgggcgggc cgccgaaagc
     4081 gaccttcgcc gaggccgacc tcgatttgta ccgtctcgcc ttcgagctga acgcgctctc
     4141 ggccgtgcgg ctcgcgcgcc tcgtcgtgcc ggggatgcgc gagcgcggct ggggacgcct
     4201 cgtcaacatc acttcggtct cggtcaagca gccgattgag gggctgctgc tctcgaacac
     4261 catccgcgcg gggctgacgg ggtgggccaa gacgctctcg aacgaggtgg ccgccgacgg
     4321 ggtgacggtc aacaacgtcg cgcccggctg gacacgcacg gcgcgacagg aagagcacga
     4381 ggcggccaac gcggcccggc tcggcaagtc gattgaggag atgatcgaag cgcgcgccag
     4441 tcaggtgccg gcgcggcgca tggcgcggcc cgaagagatc gccgccgctg tcgccttcct
     4501 cgcctccgag cgcgcctcct acatcaccgg cgtcaccttg caggttgacg gcggctgggt
     4561 acgcgggctt ttttaacagt cgtcagtaat cagtacggtg tatatacaaa atatatttta
     4621 ttgccatatc ctatccactt gagactcatt ttcttttatc acaatacgaa atacatctgg
     4681 tgtcgtaaga tgctgccatt cttcaaaacc atattttgaa tcgaagtgct ttaaaattaa
     4741 agtcagtaat cgtccgtgag taaccaccac tatcggattt gtatctaaca aaagagcgtc
     4801 attgatagca gctatggccc gatcagttgc ggcttgactc gactctcctc cttcaaagga
     4861 gagttcaaag tctgaaaaag ttcctcgcaa tttatcaggc cagtcagaat aatcaattgt
     4921 acttaatgct gcttcaatca atcgttcatc taatttaatc ttaatattta agcgaattga
     4981 taagggtttg attgatttaa gggcccgagc aaatggactg gacacaatct gaccaactcc
     5041 aactcctaac aaaaattctg caagagcctc cgcttgatct atgccttcag aggttagcgg
     5101 ggcatcagcg gcttgccccg tcgccttacc gtgtcttacc aggtatatta ttttattcat
     5161 gattactgct tatgattaga agctatttca acacgaaagt gccatccgtg aagtttatca
     5221 aaccattttt gagatggctt ctagtcagtt gtcagttgtg tgtgtgtggc gactcgccgc
     5281 tgacatcgga catctgaagg ttggaagccc gcaccactga caactgacta ctgacgactg
     5341 actactgtga gttatcaaat cgaaaacatc gtcgaggcgc ggcacgccgg cgcggaggtg
     5401 gcggtcgcgg acttcgggca cgaggcgtgg ggacgggcgc ggcccgcgca catcgctcgc
     5461 tactggtcgg gcgaggcggc gccggagggg cggcacgccg aggcgcgcgt cgtgtggacg
     5521 gacgcggcgc tcgtcgtaaa gttcgactgc cggcaggagg agccgctcgt cgtcagcagc
     5581 acgccgaacc tccgggagaa gaccatcggc ctctgggatc gcgacgtgtg cgaaatcttt
     5641 ctcgcgcccg agccgtcggc gcccgaacgt tacttcgagt tcgaggccgc gccgacgggc
     5701 gagtggatcg acctcgcgat tcactggcgc gcacaggggc gcgagactga ctggcgctat
     5761 cgctcacaca tgcagacggc ggcgctcgtc gccgacgact cgctgagcgt caccatccgc
     5821 atccccttca agtcgctcgg ccgaagcccg cgcgcggggg agcgctggcg cgcgaacctc
     5881 ttccgctgcg tcggccccga ccccgtctac cgctacctcg cctggcagcc gacgcacacc
     5941 gaagccccca acttccacgt ccccgagagc ttcggctggc tcgccttcaa gtgatgacgg
     6001 cgctgctttc aaaacaacat ccgcgactgc gcggctcacc ccttcgccag ttccctcacg
     6061 aactccccga accgcatcat gtcatccggg tccatctcca tgatgtgcgc gccgacgaga
     6121 tagccgcgct cgccgccctc ctcgtcgccg agtcgttcgt agcgcatggg gacggcgcgc
     6181 agttggacgg cgccggtggg cagttcgagc gtgatctgga cggggcggcc ctcgcccatc
     6241 aggtagcggt cgccgatgcg gatggcgggc aggacgaggc tgatgccggt ggcgctcagg
     6301 tcgcgcgtgt ggccgctgag tatgggcgcc ggccggccgg cgtggtggcg cgcgttgccg
     6361 gccgaagaga cgtgcgagac ggtggccggc aggtgcgccg ggtagcgcgg tgcgtgccgg
     6421 cggtcgctga cgaaccggcg caggtggttg acgagtgtgc ggagacgttc gggcattgcc
     6481 tcggtcagcc tcgcgtgacg cctcgctggg gagacgccgt cgggagcaag aatggacggg
     6541 gcaaatcttc gcgagcgccc ctgccgcgct cctggctcgc ggcccgacgc gggacgggcg
     6601 cccgcgcgga gcgtgtgggg gttgtcggct aacgtgtcgg cttacgacgg acgcggggac
     6661 ggccttcatt ctacgagcgg gggcgcggcg gcgcaatccc gcggcactga cggctcagtt
     6721 tcccttgagg ggcttgccgc ctttgtcgtc cttcttaagc cccgacgggt cgatgtagaa
     6781 ggagaggcga cccgtccggc cgtagcgcgc cggctcggcc ccgcccgtgt agctcttgcc
     6841 gcccggcgcg gtctcgacgt ggaagtggta gccggtcgat gcggtggaga gcacgtcggc
     6901 ggggataagg gcggagcgca cgagctgttg gaggttgccg aagaggccgc cgttttgcgc
     6961 gctgtagata atctgcgccg tcgagatgcg ccgcagcatg ttctcggcgt cgtcctgatg
     7021 ggccgccatg cgcgcctcga agaaatattt cttgccctgc ttcttgacga gcttctgtcc
     7081 ctcgcggtct ccgaagagcc acgcgccgcg ctcgcggatg aggggcagtg gcacgacctg
     7141 cggggcttcg tccgtgccga gtttcataaa gacggtcgcg tcgtcgccgc tcatctgctc
     7201 gccggtgagg gccacctgct cgggcacctc gcccgcctgt cgctcgaagt ccggcttcaa
     7261 gtcctcgtac tccgccgcgc tcagcccttc gagcgccggt gcgaagatgg agagcgcgaa
     7321 cgcctcgcgg aaccggcgct cgcgcatcag ccggtagaac tcctgcaccg cgaaggacgg
     7381 cgagccggga gccgccttct gctgcggcgg cggcggcgcg gcgggggccg ccggctgctg
     7441 cgcgacagtc ctcattccga gaccgggcgc ggcccacgac gcgagacaaa gcacgagggc
     7501 gagggtcggc ggaggcaaag acggaagtgt gcgagagcgt gggtggcgtg tcttcatctg
     7561 tggttcgttt ccttcggcgc cggctgaagt ggggaaagcg acgcgctttc gatattacat
     7621 gagccgcgcc ccgggggaga aggcaaaagg taaaaggcaa aaggcaaaag ttataaaggc
     7681 tgcaccggag gcgttcatca gccgccgctc cacggcgcgc tgacggctgc gaccctgcct
     7741 cttcactttt gcctttggcc tttttacttt tgccttccca ttacggttcc ggcgcggcgg
     7801 cctcgacggt cttcgtctcg ccggcgcgca gggagacgcg cgtggccccg gccgcacgct
     7861 tcctgacttc ctcctcgaag ccgcccacga gcgacgacgg cgtcagcaca acgacgacgg
     7921 cgtagtcgcc cggcgcggcc ttgatctcgc aggagtcgcc ggcgcgcgtc gagcagtgga
     7981 actgttgtgc gtcgtgggcc gaccagcggg ccgcgtcggc gctcacgagt agcacgtgga
     8041 cgccgacgcc cgccgtcttc tccgaggccg cacggacgtt caccttgagg gtcgccccgt
     8101 cggaagtgta gaccatgctc acgccctcga ccgccgcgcc ctccgcgatg tcgagcggct
     8161 cgcgcatcag gtctttgccg cgccacgtga tcgacttgag gtggaaggtg ttgccgtcgc
     8221 ggtcgtagaa gctcgcgtgg aggtggtact tccccggccc catgccgtcg agcgtgaacc
     8281 ggcccttgta ggcgctcgcg ctcgcgctcg ccgtcgcctc gaccgcgccc gagaggccct
     8341 tcccgacccg cgtcgtgtgg acatgtacgt aatcggggag cggccggctg ccctcgaagc
     8401 tgatcgtccc ggagacgcgc ccgccctcgc ccagttcgat tgccagctcg gacacgtcgc
     8461 cgcccgcgac ctcgacttcg acgcgcgcgg gcgcgtactt cttcacgcgc cgcggcgggt
     8521 cgttgacgat ggccgtgttc aagttcccga ccgttatgtt cgtattcatc tccatgaccg
     8581 tcacgttcgc gttgctgatg cccgctgtcc cgtccggggg aaagagctgc ggtgtcggct
     8641 cgtaggcgtc gggcggcttg acgaagatcg tgtacgaccc gtcggggatg tccttcagaa
     8701 cccagcggcc ttcctcgtcc gtctgcgccc cgttctcgaa gacctcgtcg agcgacgcga
     8761 gttcgaatgc accctgcgcc gcgtctggcc gccgggggaa gatcgccacc atggcccgcg
     8821 ccacggggcg gcggtcgcca cggccgcgga cgacgccggc cacggtgcgc gaatcgcgct
     8881 ccgccaccgt gatgtcaacg tcctcgcgct cgtcgccggc cccgaccttg atgaccgtcg
     8941 cggccttctc cgtcgtcgcc gacgggtgga aggtcatgta gagcgactgg ccgaagaggc
     9001 cgcccacctc ggccaccccc tgcatgaaga aggccgagcg cccctcccct gcttcgttgt
     9061 gctgcgccga ctcggagacg gcgacgacgt attcgccggg cggcataccc gcgatgcgga
     9121 agcggccgcg gtcgtccgtc ttcgagccga tgctgaaacc cgtcagcgtc ttcgcgaagc
     9181 gtcctccggt ctttcgcatg aggtggacgg tgaggccgac ggccggctcg ccgtcggagt
     9241 aggtgacgcg cccgccgagg gccgcgccgc ggcgcgcgcg caccgtcacc tccttgtcgg
     9301 ccttgcccgt cgtctcgaac acgtcgaagt ttttgacgat ctcgtcgaag ttgaggaagt
     9361 cggagcgcac actttcgagc gcggcgaaac tgatggggga gatgacgccg gccacgtcca
     9421 ccgcggcgat gtagcggcca gcgctcaccc ccttgatgcg gaactcgccg cgcgcatccg
     9481 tcagccccat caactgctcc ggcccgcgcc cgtcgggact gagcagcatg atgcgcgcgc
     9541 ggcggacggg gcggttcgtg ccctcgtaga cgacgcggcc gtagacagcc gccttcggcg
     9601 tcttgtcgcg tcgtccctcc tgctgcgccg cgcccgcttg cgcgtgcgcc ggcggcgatt
     9661 cgatgcccca cgccgcgaga cacacgcatg cgagggaggc gagggcgagc gcgacgaaga
     9721 gcctgtcgga gacggcgcgc aaaagtttgg tcttcatgtg ttcgactcgg cggacttctg
     9781 aagggctgtc cgcgaaagcg aaaaggttag ccgtgagtgc tctcgggcgg cgctcacggc
     9841 taaccatcta atcaacactt cggccagcgc cgcgcgtcgg cccctttagg aaatatcccc
     9901 gacgcgcctt ccggcgagca gtttctcgcg cccgacgagg tagagcagga cgacgaggat
     9961 gccgaacggg atgagcgcca gcacgtcggc ccacggcccg gcgtggaacg ggttgttcgc
    10021 ctcggcccat tcgacgaacc ggcggttcgt gtccgtcagc acgccggcca tgaaggcgat
    10081 gatgatgccg gcaatcgcgc cgccggcgat gtagcccgag gccatcaaca cgcccggact
    10141 cttgtcgccc tcggcgacca gctcgtcctc cgtcagattg cggtgcgcga gccgctgccg
    10201 cagcttcttg tccaccagat agcgcacgag cccgcccacc agaatcggac tcgaagacga
    10261 aagcggcaga tagacgccga cggcgaaggc cagcgacggg atgccggcca tctccagcac
    10321 caccgcgatc atcacgccga ggagcaccag cccccacggc agctcgcggt tgaggatgcc
    10381 cttgatgatg tagctcatca gcgtggcctt cggcgcgtcg tacttgtcca cgggcgtgcc
    10441 gtcggcgcgc gtcctgatgt tgccgttgat gccgggatcg acgcggtaga cggggacgcc
    10501 ctgctcgttg acgagatact tgccggcggg gctgcccgtc tcgtcaatct tgtgccagac
    10561 gcggtactgc tgcgtgtccg aaatcccctg cgcggccgga accgtctcgc gctcggtgag
    10621 ctgcgcgggg tcgggggcgc gcaccgtcgc gggcaggctc gcctgcgcgg ccgggacgta
    10681 gacggtcgag gcgtcgttga ggcggagcag aatcgggccg aggatgaggg ccgaggcgag
    10741 cgcgccgatg aggatggcga tttgctgccg gtagggcgtg ccgccgacca gaaagccggt
    10801 cttcaaatcc tgcgaggtgg tgccgccgtt cgaggaggcg atgcagacga tggcgccgat
    10861 ggagagcgcg gtgacgtagt agctcggccc cgtccagccg atgaagagga agacgagaca
    10921 ggtcaaaagc agcgtcgcca ccgtcatccc cgagatggga ttcgacgacg agccgatctc
    10981 gcccgtcagc cgcgacgaga ccgtgacgaa caggaacccg aacgcgatga ttaaaatcgc
    11041 gccgaggatg ttgaactgga ggttgagctg cggcagcacc atgatcatcg cgagcagcgc
    11101 gacgatgccg gccagcacga acttcatcga gaggtcttga tccgtgcgcg gggagcgcgc
    11161 cgtctgcgcc tgcccgccgc gcaggtcggc cagtccggcg cgcaggccgt gccagatggt
    11221 cggcaagctg cggacgaggc tgatgatgcc gccggcggcg accgcccccg ccccgatgta
    11281 gaggacgtat gcgccgcgga tgccgttcgg cgtcatctcg ccgatgggcg tcgtgcccgg
    11341 cgggatgatt gcggagccga ggaagtcgcc gaagaaggag atcatcggga tgagcaccat
    11401 gtaagcgagc acgccgcccg ccgccatgat cgccgcgatg cgcggcccga tgatgtagcc
    11461 gacgccgagg agcgcgggcg aagcctcaac cgccaccgag ccgttgcggt agggcgcgcc
    11521 gaaaatcttc tccggcactt ccttccacaa gtagaacgac ttcatcagcg tcgtgtagag
    11581 caggccgatg ccgaagcccg tgaagatggt cttggcgccg gtgttcgtgc cgccgcgctc
    11641 gtccgcggcg tcgcggtaga gcgggtcggc cgcggcgcgc gagtcctcgg tcgcgcccgc
    11701 cttcagcact tcggcgcacg ccgtcccctc cgggtacttc aggatgccgt gctgctgcac
    11761 gatgagcgcg cgcctcaagg gaatcatcat caggatgccg agcagtccgc cgagcacggc
    11821 gacgagcatc acgcgcgtca gttccagatc gaagcccaga atcatgatcg ccggcatcgt
    11881 cacgccgacg ccgaaggcga tactctcgcc ggccgagccg gccgtctgaa cgatattgtt
    11941 ttcgaggatg gtcgagttgc gcgcgccgaa gccggcgaag acgcggaaca gcgtgatggc
    12001 gatgacggcc acggggatgg acgcgctcac ggtcaggccc accttcagca cgaggtagag
    12061 cgacgaagcg ccgaagacca tgccgaggat ggtgcccacg acgacggcgc gcacggtcaa
    12121 ctccggcatc tcgacgtgcg ccggaatgta cgggcggaag gaggcggcga gcggattttc
    12181 cgggcgcgcc ccgacgatgt ccgggtactc gttcgtcacg gccacggact tcatggtgtt
    12241 agcgtcagtc gccatcaggt cagacctccg actatcgttt attctcgcct gcgttggaag
    12301 agactgttac ggggaaaaac tgccgcctac tttacacgcg ctccgcgcgc ggaatcaagc
    12361 cccttttggg cgcggcggcc ccgcccccgc tcgtgtgata cactgcgcgc tcgccggctc
    12421 ttccatgaat ccactcttct tcggagactg ctgatgttcg cacgcgctct cgcccccgcg
    12481 ctcgcctccg cgctcgccct ctcttcgaca ctctctttac aggcacgtac acaacgctcc
    12541 tccgcgggcg aggcggcccg cgctcgctcc acggcgcagg gcggggcgcg cgtccgcgcg
    12601 gcggcggccg tgcccgcgcc cgagtccgtg ctcggcttcc gccccggcga cgaccgcaag
    12661 ctcgctgctt gggcgcaggt ggtcgaatac ttccgacgcc tcgacgcggc cagcccgcgc
    12721 gtccgcttcg aggagctggg caagacgacg ctcggcgcgc ccttcgtcgt cgccacgatc
    12781 agcgcccccg agaacctcgc gcggctctcg gagtacaaag acatccagcg ccgcctcgcc
    12841 gacccgcgtc tgctcgggcg gcggcccgac ggccgcgccg cgtcgctcgt cgcacgcggc
    12901 aaagcggtcg tcctcatcac ctgcggcgtc cactcgacgg aagtcggctc ctacctctct
    12961 tccatgctca tcgcgcaccg cctcgcctcc tccgacgacg ccgaggtgcg cgagattctg
    13021 ctcaacacca tcgtcctgct cgtgccgtct ttgaaccccg acggcgtgga catcgtcaaa
    13081 cgctggtacg accgcacgct tggcacgccc cacgagggaa cctccccgcc cgagctttat
    13141 cacccttacg tcggccacga caacaaccgc gactggtacg ccttcacgca agcagagacc
    13201 cgtctcaccg tcgataagat tcacaacgtc tggcgcccgc agatcgtcca cgacattcac
    13261 cagatgggtt cgaccggcgc gcgcatcttc ctgccgccct acatgcagcc cgtcgagccg
    13321 aacgtgccgg tgccggtcgt cgaggcttac acggaactcg gccgcgcggt cgccgctggc
    13381 ttacgcgcca aaggcatgaa gggcgtcgtc accgacgcca tctacgacgc ctggactccg
    13441 gcgcgcgcct actcccacta ccacggcggt gcgcgcatcc tctccgagac cgcctccgca
    13501 cgcatcgcca cgccgacgac cgtgaagccc gaacagcttc gcgccggctc cgacgacggc
    13561 tacgacccgc gcaaggagtc agcgaacttc cccgacccgt ggcccggcgg cgactggtct
    13621 ctgcgagaca ttaccggcta catgacgagc gccgccttcg agttgatgaa acacgcggcg
    13681 cagaaccgcg agcattggct gcggcgcttc tacgaagtcg gccgcgaggc cgtgcgcccg
    13741 cgccggcgcg gcgagcttgt cgggtacaag attctgggcg gcccgaacac caacgccctg
    13801 attgaaatcc tggatcgcgg cggcctggag gttcgcaacg tccccgtcaa gacgccgcgg
    13861 aggggccgcg gccgtcgctc ggcgatggac gaattggggc cagacctctt cgtccccctc
    13921 gcccagccct acggcggctt cgccaaggcg ctcctcgaaa aacagaacta ccccaacctg
    13981 cgcgacccgg ccggcaaccc catccaaccc tacgacgtga cggcgcacac gctgccgctt
    14041 ctgatgggcg tcgaagtcga gccggtctat gagccgatac gcaccgtcgc gttgccgacc
    14101 gtccgcgtcg gcagcgccga gcgcgatggc gtctcattcg tcatgcctcc cgccgagccg
    14161 gcccgaccgc aagcgcaggc acgcgtcggg ctttacaaat cgcacgtcgc atcgatggac
    14221 gagggctgga cgcgctgggt gctggagcag gagggcttca agtatcgctc gctgctcgac
    14281 gcggaggcgc gcgccggttc cttgcgcgcg aagtacgacg caatcgtcct ccccgaccag
    14341 tcggcgcgcg ccatcttcga gggacaccgg aagggcacga tgcccgacga gttgacgggc
    14401 ggacttggcg aggagggcgt ccgtgcgctg cgcgagttcg tcgagcgggg tgggacgctc
    14461 gtctgtctga acagcgcgtc gaatttcgcc atcgagcact tgaagctgcc ggtgcgcaac
    14521 gcgctcgcgg gccttcgccg cacggacttc ttcgtgcccg gctcgattct gcgaatcgtg
    14581 ctcgacaagt cgcacccaat cacggaagac atgccgcagg agtcggtcgc gtggttcgag
    14641 gactcgccgg ccttcgagat cgcgggcgac ccggccgcgc tgccccgcgt aaaaatcgtg
    14701 gcgcgctacc cgcagagcgg gaaccctctg ctctcgggct ggctcctcgg cgagcagcat
    14761 ctgcgcgggc gcgcggcgct cgtcgaggtc ggcatgggtg aggggcgcgt gatacttttc
    14821 ggcttccgcc cgcagtaccg cgcgcagtcg ctcgccactt atccgctgct cttcaacgcc
    14881 atcgcgcacg ggggttcacg gccgtagagt ggggcggccc cgcagggcgc gagaaaagga
    14941 cacgcgtttc tcgcgcgcgc agcccgtcgc ggcccccagc attcctgacc gagtgaggag
    15001 aacgagatga aacgtagaac gatcactctc ccggcgctcg ccttcgcggc cctggcgctc
    15061 ctgccgctaa cagtggccgc gcagcagccg gcggcgcaga gacagtccgt tgacctcggg
    15121 gcgttcacca aagagatcat gctcctgcgc ttcgacggca cacagcagca cctcgtgatg
    15181 tggctgcctt acgagttctt cgtcgccgcg ggcacggccg acggcacgat gacgagggag
    15241 tcgatggaga aggagttggg gtttctcaag tcgtacctca cgctcttcgt gatgagcagc
    15301 atcgagcgcc ccgacggcgc cagcgtgtac gcgagcgatg ccgaggcgcg taagcgcgcg
    15361 gggctaaagc tggcggacgg gacggaggtg cgcccgctcg acgtggcgcc gccgaagctc
    15421 acggccatcg tcgccgcgat gaaggcgctc atggcgcagc agggaggcgc cgaccgggag
    15481 aacatgcacg tcctcgtctt ccccgccgcg acgagcgcgg gcaagacggt cgtggacgtg
    15541 acgcgcaggg atagtctcac ccttttgctc aaggccgacg cccggttccg cgaagcgacc
    15601 ttcacctggc gcactccctt cgacgcgctg acggccgtcc ccgactgccc gcgctgcaag
    15661 gccggcgtct ccgccaagtg gacttactgc ccctactgcg gccagaagct cccgcagtga
    15721 ccaccgggca ggcaacggca gtagcccgac cgtgagggag ggcgtcctca
//
LOCUS       DASVES010000203         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_683560, whole genome shotgun sequence.
ACCESSION   DASVES010000203 DASVES010000000
VERSION     DASVES010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683560"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3375)
                     /gene="mfd"
                     /locus_tag="VF240_08040"
     CDS             complement(<1..3375)
                     /gene="mfd"
                     /locus_tag="VF240_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX7175219.1"
                     /translation="MSSSVETVSVLQGVLRRVTEWSEFGRLAAEVAGGARVVSVGGLT
                     SAPARALAVAALRRETGKQLAVVVQANRDLEAWERDLSFWAGALGEDPRGGVLTLPAS
                     EGDPYAGTSPHADTLERRALSLWRLARGDAPFVLLSARALVRRTLPPARLLEAGAVLR
                     RDEDYAPEELVEKLLATGYVREDPVGAVGEFSIRGGILDLWSPGQDSPVRVEFFGDTV
                     DSIREFDAETQLSVAQVRETEVVPMREYAVSSGDFRLWAEAARERWADERRGRALKDR
                     TVHAGEGETFAGWEWLIPLLLTTDASALDYLSAETVLVVDEPAGVEQFLGSTYDTLAA
                     RFEENEAADEIGLRPDELYLTAEELRERLRDARRVELRALGRAAAVTDERFVGEAEQP
                     QVQIGRARAQGAPMFLFPAVERAPEVELRSLPARRYHGRVPDLAADVRRRYEERRAAT
                     LLVMPSAGVAERIGEVLADYNVAARVALAGEDASALGEETGQAVVTSGRLSGGFELPE
                     AALVVHVETDLFDEVSDAAVERRAPGTGERQKEKGKRKKSNAAAFLSDFRDLKVDDYV
                     VHIDHGIARFGGLQTLDLGGGRSGEFMLLFYADDSKLYAPVERLDLVQRYSSAEGHTP
                     TLDRLGGIGWQKTKAKAKRAMRDMADELLRLYAERKLVGGYAFAADSPWQREFEEGFE
                     YQPTPDQETAIEDVKRDMEAATPMDRLLCGDVGYGKTEVAMRAAFKAVMDGKQVAVLA
                     PTTVLAYQHYETLRARFAPFPVTVELLSRFRSAREQKPIVQRAEAGEIDIVIGTHRIL
                     SKDIKFRDLGLVVVDEEQRFGVAHKERLKQLKKKVDVLTLSATPIPRTLNMSLMGLRD
                     MSVIETPPRDRLAIQTQVVQFSESVVKSAIELEIGRGGQVFFIHNRVETIDTVAALVQ
                     RLVPQARITVAHGQMNEKEMEQVMLDFVAYKADVLVATTIIENGIDIPRANTIIINRA
                     DNYGLSQLYQLRGRVGRSNRRAYAYLLIPSETELTPIARRRLAAIREFSDLGAGFRVA
                     ALDLELRGAGNLLGGAQSGHMDALGFDLYTQMLERTMSELRGEDVEDEASVSISLGVD
                     VAIPESYISDMGQRLRTYKRVASARDDETLSQIRRET"
     gene            complement(3511..4017)
                     /gene="rfaE2"
                     /locus_tag="VF240_08045"
     CDS             complement(3511..4017)
                     /gene="rfaE2"
                     /locus_tag="VF240_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX7175220.1"
                     /translation="MDEKILRGRDEVRRVRASLRAAGRRLVFTNGCFDLLHVGHTRYL
                     QQARLLGDALLVAINSDRSVRALKGPGRPVMCEEERAEVLAALAVVDYVTVFDEESPR
                     ALIAEVLPDVLAKGGDYALEEIHGREEVERAGGRVVALPFVDGVSTTDVIERIKSAAE
                     RGPAEKEG"
     gene            4155..>4775
                     /locus_tag="VF240_08050"
     CDS             4155..>4775
                     /locus_tag="VF240_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX7175221.1"
                     /translation="MSLPPSILEDNDQTRARREHLEAARALVGNAYPNKFRRTNVTAS
                     SEGEDTVTALVREFRKHEPEVEAGARPTPEQLEGANAELSKVSVRVSGRLATPPRVMG
                     KAAFVHLSDGRERLQIYVRRQEAKAVSNDTGAPLDDPEGGWKLFQLLDHGDFVGVDGY
                     LFVTKTGELSIHVRELQFLAKALLPMPDKMHGVADPEIRQRQRYVDL"
BASE COUNT          798 a         1710 c         1485 g          782 t
ORIGIN      
        1 agtctcacgg cgtatctgcg acagcgtctc gtcgtcgcgc gccgaggcga cgcgcttgta
       61 ggtgcgaagc cgctgcccca tgtccgagat gtaactctcg gggatggcca cgtccacgcc
      121 gagactgatc gagaccgaag cttcgtcctc cacgtcctcg cccctcagct ccgacatcgt
      181 gcgctcaagc atctgcgtgt aaagatcgaa gccgagggcg tccatgtgcc ccgactgtgc
      241 tccgcccaga aggttgcccg cgccccgcag ctccaaatcc agcgccgcca cccgaaagcc
      301 cgcgcccaaa tccgagaact cgcggatggc ggcgaggcgg cggcgcgcaa tcggcgtcag
      361 ctcggtctcg gacggaatca ggaggtaggc gtaagccctt cggttcgagc gcccgacgcg
      421 gccgcggagc tggtaaagct gcgaaagccc gtaattgtcg gcacggttta tgatgatggt
      481 gttggcgcgg gggatgtcta tgccgttttc gataatcgtt gtcgccacaa gcacatcggc
      541 cttgtaggcg acgaagtcga gcatcacctg ctccatctct ttttcgttca tctgcccgtg
      601 ggcgaccgtg atgcgcgcct gcggcacgag cctttggacg agcgcggcca cggtgtcgat
      661 ggtctccacg cggttgtgga tgaagaagac ctgcccgccg cgtccgatct ccagctcgat
      721 ggccgacttg acgaccgact cggagaactg cacgacctgc gtctggatgg cgaggcggtc
      781 gcgcggcggc gtctcgatga ccgacatgtc gcgcagtccc atcagcgaca tgttgagcgt
      841 gcgcgggatg ggcgtggcgg agagcgtcag cacgtccacc ttcttcttca actgtttcaa
      901 acgctccttg tgcgccaccc cgaagcgttg ctcttcgtcc acgacgacga ggccgaggtc
      961 gcgaaacttg atgtccttcg acagtatccg gtgcgtgccg atgacgatgt cgatctcgcc
     1021 ggcctcggcg cgctgcacaa tcggcttctg ctcgcgcgcc gagcggaatc gcgagagcag
     1081 ctcgaccgtg acggggaacg gcgcgaagcg cgcgcgcaaa gtttcgtagt gctgataggc
     1141 gagcacggtg gtcggcgcga gcacggccac ctgcttgccg tccatcacgg ccttgaaggc
     1201 ggcgcgcatg gcgacctcgg tcttcccata ccccacgtcg ccgcacagca gcctgtccat
     1261 cggcgtcgcg gcctccatgt cgcgcttcac gtcctcgatg gccgtctcct gatcgggcgt
     1321 cggctggtac tcgaagccct cctcgaactc tcgctgccac ggcgagtcgg ccgcgaacgc
     1381 gtagccgccg acgagcttgc gctcggcgta gagtcgcaaa agctcgtcgg ccatgtcgcg
     1441 catggcgcgc ttggccttgg ccttcgtctt ctgccagccg attccgccga ggcggtcgag
     1501 cgtgggcgtg tgcccctcgg cgctcgaata gcgttggacg aggtcgagcc gttcgacggg
     1561 cgcgtagagc ttcgagtcgt cggcgtagaa gagcagcatg aactcgccgg agcggccgcc
     1621 gccgaggtct aaggtctgca agccgccaaa gcgcgcgatg ccgtggtcga tgtgaacgac
     1681 ataatcatcg accttcaaat cacgaaagtc cgacaggaac gccgccgcat tcgacttctt
     1741 ccttttgcct ttttcctttt gcctttctcc cgtgcccggc gcgcggcgct cgaccgccgc
     1801 gtcgctcacc tcgtcgaata aatccgtctc gacgtggacg acgagggccg cctccggcag
     1861 ctcgaacccg cccgacaggc gtcccgaagt gacgacggcc tgccccgtct cctcgccgag
     1921 tgcggatgcg tcctcgcccg cgagtgcgac acgcgccgcg acgttgtagt cggcgagcac
     1981 ttcgccgatg cgctcggcca cgcccgcgct cggcatcacg aggagcgtcg cggcccgcct
     2041 ctcttcatat ctgcgtcgta catccgcggc gaggtcgggg acgcgcccgt ggtagcggcg
     2101 cgcgggcagc gagcgcagct cgacctccgg cgcccgctcg acggccggga agagaaacat
     2161 cggggcgcct tgcgcgcgcg cgcgcccgat ttgaacctgc ggctgttcgg cttcgcccac
     2221 gaacctctcg tcggtgacgg ccgccgcgcg tccgagcgcg cgcagctcca cgcgtcgcgc
     2281 gtcgcgcagg cgctcgcgca gctcttcggc cgtcaggtaa agctcgtcgg ggcgcaggcc
     2341 gatttcgtcg gcggcctcgt tctcctcgaa gcgcgccgcg agcgtgtcgt aggtcgagcc
     2401 gaggaactgc tcgacgccgg ccggctcatc gacgacgagc acggtctcgg ccgacaggta
     2461 gtcgagcgcg ctcgcgtccg tcgtgagcag cagcggtatc agccactccc agcccgcgaa
     2521 cgtctcgccc tcgccggcgt ggacggttcg atctttcaac gcgcgcccgc gcctctcgtc
     2581 cgcccaacgc tcgcgcgccg cctcggccca cagacggaag tcgcctgaag agacggcgta
     2641 ctcgcgcatc ggcacgacct ccgtctcccg aacttgcgcc acggagagct gtgtctccgc
     2701 gtcgaactcg cggatggaat ccaccgtgtc gccgaagaac tccacgcgca ccggcgagtc
     2761 ttgccccggc gaccagaggt cgagaatccc gccgcgtatg gagaactcgc cgaccgcgcc
     2821 cacggggtct tcgcgcacgt agcccgtggc cagaagtttt tcgaccaact cctcgggcgc
     2881 atagtcctcg tcgcgccgca ggacggcccc cgcttcgagc agacgcgcgg gcgggagcgt
     2941 ccgtcgcacg agggcgcgcg ccgagagcag cacgaagggg gcgtcgccac gagcgagacg
     3001 ccacagagag agcgcgcggc gttcgagcgt gtcggcgtgc ggcgacgtgc ccgcgtaagg
     3061 gtcgccctcg gaggccggca gcgtcagcac cccgcccctt gggtcttcgc cgagcgcgcc
     3121 cgcccagaaa gacaaatcgc gctcccacgc ctcgaggtcg cggttggcct gcaccacgac
     3181 ggcgagctgc ttccccgtct cgcgccgcag ggccgcgacc gccagcgcac gcgccggcgc
     3241 agaagtcagc ccgcccaccg acacgacgcg cgcaccgccc gccacctcgg ccgccagccg
     3301 cccgaactca gaccactcgg tcacgcgccg cagcacaccc tgcaacaccg aaactgtctc
     3361 gacagaactg ctcataactt tgaagctgtt agctattagc tgttagctgt tagctttcag
     3421 ccagaagagg acgagccggg gcttcgttca tcagaagcgc cttcggctcc cgtttggctg
     3481 actgctaaca gctaatagct aacagcttca tcacccttcc ttctccgccg gccccctctc
     3541 ggccgccgac ttgatacgct cgatgacatc ggtcgtcgag acgccatcga cgaaggggag
     3601 cgccaccacg cgcccgccgg cgcgctccac ctcctcgcgc ccgtgtatct cttcgagcgc
     3661 gtagtcgccg cccttcgcca aaacgtccgg cagcacctcg gcgatcagtg cgcgcggcga
     3721 ctcctcgtcg aagaccgtca cgtaatccac gaccgcgagc gccgccagca cttcggcgcg
     3781 ctcctcttca cacatcaccg gacgccccgg ccccttgagg gcgcgcaccg agcggtcgct
     3841 attgatcgcc accagcagcg cgtcgcccag cagccgcgcc tgttgcaggt agcgtgtgtg
     3901 cccgacgtgc aggaggtcga agcagccgtt ggtgaacacc agccgccggc ctgccgcgcg
     3961 caaagacgcg cgcacgcggc gcacctcgtc ccgcccccgt aagattttct catccattgc
     4021 cgtttcgcca atcgtaaatc gtaaatcgcg aatcgtaaag cgaagattcc gccgattcac
     4081 gatttacgat ttacgattca cgacttcctt actctataat ccgcgaacac tcgcgcacga
     4141 agccgaaaac aaacatgagc ctgccaccat ccattctcga agacaacgac cagacgcgcg
     4201 cccgccgcga gcaccttgag gccgcgcgcg cgctcgtcgg caacgcctac cccaataagt
     4261 tccgccgcac gaacgtcacg gccagcagcg agggcgagga caccgtcacg gcgctcgtcc
     4321 gcgaattcag gaagcacgag ccggaagtcg aggcgggcgc gcggcccacg ccggagcagc
     4381 ttgagggggc gaacgcggag ctaagcaaag tgtcggtgcg cgtctccggt cgcctcgcca
     4441 cgcccccgcg cgtgatgggc aaggccgcct tcgtccacct ctcggacggc cgcgagcgtt
     4501 tgcagattta cgtccgccga caggaggcga aggccgtcag caacgacacc ggcgctcctc
     4561 tcgacgaccc cgagggcggc tggaaacttt ttcaactgct cgaccacggc gacttcgtcg
     4621 gcgtcgatgg ctacctcttc gtcacgaaga cgggcgagtt gtcgatacat gtgcgcgagc
     4681 tacagtttct cgccaaggcc ctgctgccga tgcccgacaa gatgcacggc gtcgccgacc
     4741 ccgaaatccg ccagcgccag cgctacgtcg atctc
//
LOCUS       DASVES010000204         8801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1184859, whole genome shotgun sequence.
ACCESSION   DASVES010000204 DASVES010000000
VERSION     DASVES010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184859"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..2083)
                     /locus_tag="VF240_08055"
     CDS             complement(332..2083)
                     /locus_tag="VF240_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX7175222.1"
                     /translation="MIRMHALLLRAALLSLAAACAAGCTTEQPAAVNQNTSPSPAAPA
                     AMNVSADLKPAVESLTAADMLRHTTELSSDAYEGRGPGTKGEELTVKYLTEQFQRLGL
                     KPGNPDGTYVQKAPLVGLKAEPTATLSAGGKEMALKFPDDYVAVTRRTQPDVKVTNSD
                     VVFVGYGVVAPEYGWDDYKDVDVRGKTIVMLINDPAVTDPADASKPDEKMFKGKAMTY
                     YGRWTYKYEIASAKGAAAAIIVHETETAGYGFEVVKSSWGRENFDIKSADGGAGRVGV
                     ESWITLDRAKELFTASGKNFDELKKAALTREFRPVALGAKANFQIKNTVREVQSQNVI
                     ARLEGSDPVLKDEYIVYTAHWDHLGRDESKQGDQIFNGAIDNASGTAMLLELAEAFTK
                     LPTPPKRSVLFLAVTAEEKGLLGAKFYAENPLYPLAKTLANVNVDGVNQWGRTRDIVI
                     IGLGNSTLDDLTQEVASLQSRVVKPDPEPEKGLFYRSDHFEFAKQGVPALYTDTGTDY
                     VGKDPAFAMQKRAEYDKNDYHSPTDEVKPDWDLSGAVEDAQLLFVIGHRVAQGDKFPE
                     WKPGAEFKAKREATLKK"
     gene            2664..5372
                     /locus_tag="VF240_08060"
     CDS             2664..5372
                     /locus_tag="VF240_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175223.1"
                     /translation="MKSAKHLKIFSAVALAVLLLLAPLASAQRRRARAPRRAASTRAA
                     QKPAARAQQPPQPQPTPAAAGVRRQPARTAVPPVVREAEDERGATEIDLPLEEVVAAE
                     GYALYIELRALGHLVRTKELTELIELVRLVMVGSEARQVSEYIDFFTANAEQLNDVNA
                     YTLMMPTRAGLPQFVGAYQFPSVEVAKNFEPKARAVMQKYERAYREVFPTPNPAEPTG
                     ATTTVAEAGKQGAGAATPATPDPKIVVKRYGRLILMSGEPFTLKSLKGKDEPPLTHNT
                     RFQTLRSRLSSEPVFLYFDVKLLDKSEAADRERREQAQKEQETLNGSGPGQAAAQEEE
                     AERRMREVMAQAEAEAQIAEAARESGPAEEGEEEGEGDPEMMAPPEVAVESSAVTVET
                     TPSPTLSGRMEGGQAGGDDGAAAGRGGEFMLSMLFGMGMSGASRWPEAVGAALALERD
                     TFVIRALLLNSAGAQLHPVPFLPVFISGPQLTPQAASLAPANTDIYVSASLDLAQMYD
                     RMLASMDESFSREAQAREATGEKMSDERAGNKISDERPDKAEATIAAAEKLLGFKIKG
                     DFLPALGNEVAVSVPLKWVMGESGHNHGYRPEAKDERQGAVVLVSLNNAEAMRRMLPR
                     VLEVMGLKPLTAAEQKVSHGGVDIHVYGSLAVAYIGNFVALSSEVPAMKRLIDSGSSQ
                     GALAADEAFRTATSWSPRQKLAEVYVSRALADSLLTEVTKWVDPKDAEVQQMLARLNI
                     RPAPASYAATDEGGMLLHELRLPTAIVKYFAAGAMLETKTGPVKRGETSALSTLTMIR
                     HMQQAHKDGKGKGSYATLDELAAPPARRASRHGAEWEHPLSKLALEKQEYRFELTAAG
                     DKYAVTATPREFGKNGRRSFYMDETGIIRATERAGEPATANDPPVD"
     gene            5459..5923
                     /locus_tag="VF240_08065"
     CDS             5459..5923
                     /locus_tag="VF240_08065"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7175224.1"
                     /translation="MNEKSLQETHSPASACFGCGPVNERGLRIRSFARGDEVVAEWQP
                     EPHHEAYPGMLNGGIVGTLLDCHSNWAAIHHLMLKGGLDKAPCTVTADYSIKLLRPTP
                     TDAPLRLAARVVSSTEDRATVEAELIGHGKICATCRGTFVAVRPGHPAYHRW"
     gene            6161..8188
                     /locus_tag="VF240_08070"
     CDS             6161..8188
                     /locus_tag="VF240_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7175225.1"
                     /translation="MMMRERVSTDGQSSWGGPSARRGRLGHLTRFACALAAWGALLHA
                     APAVAAATLPSGFTETQVAGGLSSPTAMAFAPDGRLFVCQQGGQLRVIKNGVLLPTPF
                     VTLSVSASGERGLLGVAFDPNFASNQFVYVYYTATTPAIHNRVSRFTAAGDVALGGSE
                     TILLELDNLSAATNHNGGAMHFGEDGKLYIAVGENANGANSQTLSNLLGKMLRINSDG
                     TIPADNPFFTQAVGKNRAIWALGLRNPYTFAFQPVTRRMFINDVGQNTFEEVNEGLVG
                     ANYGWPDTEGPTTNPNFRTPFYYYGHGGGLFGGCAITGGTFYNPSTQQFPVEYGGDYF
                     FADYCGGWINYLTDPSNTNAVANFASGISFPVDLQVGPEGALYYLARGASAGAGVVFR
                     VEYTANQAPAITQHPSNQTASAGQTATFSVAASGTGPLSYQWQRDGVDINGANSSAYT
                     TPPVSAADNGARFRCLVSNAFGSATSNEAVLTVVSNRPPVATITQPAAGALYSGGDTI
                     NYAGTGTDPEDGTRPASAFTWEVVFHHDTHTHPFIPPTSGATGGSFTIPRTGETSANV
                     FYRIHLTVADSGGLTHTTFRDVRPRTVTITLAASPSGPQLTLDGQPVATPHSFVGVVG
                     MTRTIAAPSQSVGNFSYTFRSWSDGGAQTHTITTPSVNTTYTASFRKKKQQ"
     gene            complement(8358..>8801)
                     /locus_tag="VF240_08075"
     CDS             complement(8358..>8801)
                     /locus_tag="VF240_08075"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175226.1"
                     /translation="NAGAREMSDAKIGFQHGEGGGAGRGKGRGVIERTFSPEFRNRLD
                     AWIAFDALSFETTERVVDKFIGELGAQLSAKNVSIELTDGARRWLATHGYDKQFGARP
                     MSRLIQTEIKAPVAEQILFGSLQTGGTVRIEVRGEKLDLQFLNSQ"
BASE COUNT         1554 a         2959 c         2817 g         1471 t
ORIGIN      
        1 cggccgactt gggggagtcg gccggcccgc gcctttgtta aggcaaaagt gaggaagccg
       61 agtcgcaggc atcaacgagc cgcggagcgg cggcagcagt gttgccccgg tcgtgagacc
      121 ggggtcagcg gtttagaaaa cacaaggaag ccccggaggg gcgaaagaat cttcacgtgc
      181 gtcccacgtc aacgtcgagg ttctttcgcc cctccggggc ttcacttttt tcaatcctac
      241 gtgtccccac ggctcgcgcc gtgggctaca ttctgccgcc gcctccggcg gctcgtggac
      301 gcttgcgact cagcttcctc acttttgcct gctacttctt tagcgtcgcc tcgcgcttgg
      361 ccttgaactc ggcgccgggt ttccactcgg gaaacttgtc gccctgcgcg acgcggtggc
      421 cgatgacgaa gaggagttgc gcgtcctcga ccgcgcccga caaatcccag tcgggtttta
      481 cctcgtcggt cggcgagtgg tagtcgttct tatcgtactc cgcgcgcttc tgcatggcga
      541 aggccgggtc tttgcccacg tagtctgttc cagtgtcggt gtagagcgcg ggcacgccct
      601 gcttggcgaa ctcgaaatgg tcggagcgat agaagaggcc cttctccggc tcggggtcgg
      661 gcttgacgac gcgactctgc aaagaagcga cttcctgcgt gaggtcgtcg agcgtggagt
      721 tgccgaggcc gatgatgacg atgtcgcgcg tgcggcccca ctgattgacg ccgtccacgt
      781 tgacgttggc gagcgttttg gcgagcgggt agagcgggtt ctcggcatag aacttcgcgc
      841 cgagcagacc cttctcctcg gccgtgacgg cgaggaagag aacggagcgt ttgggcggcg
      901 tgggaagttt cgtgaacgcc tcggcgagtt ctaagagcat cgccgtgccc gaggcgttgt
      961 cgatggcgcc gttgaaaatc tggtcgccct gcttcgactc gtcgcggccg aggtgatccc
     1021 agtgggcggt gtagacgatg tactcgtcct tgagcaccgg gtcggagcct tcgagacgcg
     1081 cgatgacgtt ctgcgactgc acctcgcgca ccgtgttctt gatttgaaag ttcgccttcg
     1141 cgccgagcgc gacggggcgg aactccctgg tcagcgccgc cttcttcaac tcgtcgaaat
     1201 tcttccccga ggccgtgaac aattccttcg cacggtcgag tgtgatccac gactcgacgc
     1261 cgacgcgccc cgcgcctccg tccgccgact tgatgtcgaa gttctcgcgt ccccagctac
     1321 tcttgacgac ctcgaagccg tagccggccg tctccgtctc gtggacgatg atcgcggccg
     1381 ccgcgccctt cgcgctggcg atctcatact tgtacgtcca gcggccgtag taggtcatgg
     1441 ccttgccctt gaacatcttc tcgtcgggct tcgaggcgtc ggccgggtcg gtgacggccg
     1501 ggtcgttgat gagcatgacg atggtcttgc cgcgcacgtc aacgtccttg tagtcgtccc
     1561 agccgtactc gggcgcgacg acgccgtagc ccacgaagac gacatccgag ttcgtgacct
     1621 tcacgtcggg ctgcgtgcga cgagtgacgg cgacgtagtc gtcggggaat ttcagggcca
     1681 tctccttgcc gcccgccgaa agagtagcgg tcggctcggc cttgaggccg acgagcgggg
     1741 ccttctggac gtatgtgccg tcggggttgc cgggcttgag gccgagccgc tggaactgct
     1801 cggtgagata cttgacggtc agctcctcgc ccttcgttcc ggggccgcgc ccctcgtagg
     1861 cgtccgagga gagttccgtg gtgtggcgca gcatgtcggc ggcggtgagg ctctcgacgg
     1921 cgggtttcaa atcggccgac acgttcatgg cggcgggcgc ggccggcgac ggcgaagtgt
     1981 tctggttaac ggcggcgggc tgctcggtcg tgcagccggc ggcgcaggcg gcggccagtg
     2041 acagcagcgc ggcgcgcagg agaagggcgt gcatacgtat catcgatcaa agcctctgct
     2101 cggacaggat gtgaaagcga gatgctttct tctattcccc tcggggcgtt caagtcaaat
     2161 catcgccgag gacgacgtgt aaaattgtag gggcagggca agtccgcggc ccgccggacg
     2221 cgaaagaaat ctacatagat ggacaggata ggtaggatga agaaagagct gattcgagat
     2281 ttcaacgggg gcgctcatgg cagtagcccg accgtgaggg agggcggcgg ggagtcggca
     2341 gtcggcagga ggcagacggc cgtcagggcg cgtgaccgtc ttgactgccg actgctcact
     2401 gcttactgcc tactcctgac gccctccctc acggtcgggc tactgccctg tcctacgccg
     2461 aggactcatc cagttcatcc tgcccatcca tgttaattgc ttcctacgat gcgggccgcg
     2521 gacttgtccc tgcccctaca acttcccaaa gtcatttcaa aggcattacc cgtgtgttaa
     2581 tctctgacac aggaagccgt ccgttacacg gattcgcgaa cccgcctcgt cgtcgtgaaa
     2641 ttctgatgga gttttcgtcc cccatgaaat cagccaaaca tctgaaaatc ttctcggccg
     2701 tggctctggc cgtgttactg ctcctggcgc ccttagcgtc ggcgcagagg cgtcgggcgc
     2761 gtgcgccgcg acgggcggcg agcacacggg ccgcgcagaa gccggcggca cgcgctcaac
     2821 agccgccgca gccgcagccg actcccgccg ccgccggcgt cagacggcag cccgcgcgga
     2881 cggccgtccc gccggtcgtc agggaggccg aggacgagcg gggcgcgacg gagatcgacc
     2941 ttccgctcga agaagtcgtc gccgcggaag ggtacgcgct ctacatcgag ctgcgcgctc
     3001 tcggacatct cgttcgcacc aaggagttga ccgagctgat cgagcttgtc cggctcgtca
     3061 tggttgggag cgaggcgagg caggtgagtg aatacatcga cttcttcacc gcgaacgccg
     3121 agcagctcaa cgacgtgaac gcctacacgc tgatgatgcc cacgcgcgcg ggcctcccgc
     3181 aattcgtcgg cgcatatcag ttcccctcgg tcgaggtcgc aaagaacttc gagccgaagg
     3241 cgcgcgccgt gatgcagaag tacgagcgcg cctaccggga agtgttcccc acgccgaacc
     3301 ccgccgaacc gacgggggcg acgacgaccg ttgccgaagc cggcaagcag ggcgcgggcg
     3361 cggcgactcc cgcgacgccc gacccgaaaa tcgtcgtgaa gcgttacggc cgtctcatcc
     3421 tcatgagcgg cgagcctttc acgctcaaga gcctcaaggg aaaggacgag ccgccgctca
     3481 ctcacaacac gcgtttccag acactccgca gccgtctctc ctccgagccg gtcttcctct
     3541 acttcgacgt gaagctcctc gacaagagcg aggccgccga ccgggagcgg agggagcagg
     3601 cgcagaaaga gcaggagact ttaaacggga gcgggccggg ccaagcggcc gcgcaagaag
     3661 aagaagctga gaggagaatg cgtgaggtga tggcgcaagc cgaggcggag gcgcaaatag
     3721 ccgaggccgc gagggagagc ggccccgccg aagaggggga agaggagggg gagggcgacc
     3781 ccgaaatgat ggccccgccc gaggtcgcgg ttgagagttc ggccgtcacg gtcgagacaa
     3841 cgccttcgcc gacgttgagc ggcaggatgg aaggagggca ggcgggcggc gatgatggcg
     3901 cggcggcggg acgcggtggc gagttcatgc tgtcgatgct cttcggcatg gggatgtccg
     3961 gggcgtcgcg ctggcccgag gccgtcggcg ccgcgctcgc gctcgaacgc gacacgttcg
     4021 tcatccgcgc gctgcttctc aattcggccg gcgctcagct gcatcctgtg ccgttcctcc
     4081 cggtgttcat ttcggggccg cagttgacgc cgcaggcggc gtcgttggcc ccggccaaca
     4141 cggacatcta cgtcagcgcg tcgctcgacc tcgcgcagat gtacgaccgc atgctcgcgt
     4201 cgatggacga gtcgttcagc cgcgaggcgc aggcgcgcga ggcgacgggc gagaaaatgt
     4261 cggacgagcg cgcgggcaac aaaatctcgg acgagcgccc ggacaaggcc gaggcgacca
     4321 tcgcggcggc ggagaagctt ctggggttca agatcaaggg agactttctg ccggccctcg
     4381 gcaacgaggt cgccgtctcc gtcccgctca agtgggtgat gggcgaatcg ggccacaacc
     4441 acggctaccg gcccgaggcc aaggatgagc ggcagggggc cgtcgtcctc gtctctttga
     4501 acaacgccga ggccatgcgg cggatgctcc cgcgcgtgct cgaagtcatg gggctgaagc
     4561 cgctcacggc cgcggagcag aaagtcagcc acggcggcgt tgacatccac gtctacggct
     4621 cgctcgccgt cgcctacatc ggcaacttcg tggcgctctc ctcggaggtg cccgcgatga
     4681 agcgactcat cgactccggc tcgtcgcagg gcgcgctcgc cgccgacgag gcgttccgca
     4741 cggcgacgag ctggtcgccg cgtcagaagc tcgccgaggt ctacgtctcg cgcgccctcg
     4801 ccgacagtct cctcacggag gtcacgaagt gggttgaccc gaaggacgcc gaagtccaac
     4861 agatgctcgc gcgtctgaac atcaggcccg cgccggcctc ctacgccgca acggacgagg
     4921 gcggcatgct cctgcacgag ctgcgcctgc cgacggccat cgtcaaatac ttcgccgccg
     4981 gggcgatgct ggagacgaag acggggccgg tcaagcgggg cgaaacttcg gccctctcga
     5041 cgctgacgat gatccgccac atgcaacagg cccacaagga cggcaagggc aagggcagct
     5101 acgcgacgct cgatgaactc gccgcgcccc ctgcccggcg cgcttcgagg cacggggcgg
     5161 aatgggagca cccgctctcg aaactcgcgc tcgaaaaaca ggagtaccgc ttcgagttga
     5221 cggccgccgg cgacaagtac gccgtcacgg ccacgcccag agagttcggc aagaacggcc
     5281 gccgctcctt ctacatggac gagaccggca tcatccgcgc caccgagcgc gcgggcgaac
     5341 ccgccacggc caacgatcca ccagtggact agtgataagt gatgagtgat aagtgataag
     5401 aaaagccgtc tgctctttct tatcactcat cactcatcac ttatcacctt cttatcctat
     5461 gaatgagaaa agcctgcaag agacacactc gccggcgagc gcgtgcttcg ggtgcggccc
     5521 cgtaaacgag cgcggtttgc gtatccgcag tttcgcgcgc ggcgacgagg tggtcgccga
     5581 gtggcagccc gagccgcacc acgaggcgta ccccggcatg ctcaacggcg gcatcgtcgg
     5641 cacgctcctc gactgtcatt ccaactgggc cgccatccac cacctcatgc tcaagggcgg
     5701 cctcgacaaa gcgccctgca ccgtcaccgc cgactactcc atcaagctcc tccggcccac
     5761 cccgaccgac gcgccgctgc ggctcgcggc ccgcgtcgtc tcctccacgg aagaccgcgc
     5821 cacggtcgag gccgaactca tcggccacgg caaaatctgc gccacctgtc gcggcacctt
     5881 cgtcgccgtc cgccccggcc acccggccta tcatcgatgg taaaacaatc gtcgctttaa
     5941 cgaggcgatg aattcggact cgcccaacgc cgcacagtgc atggaagctc ttcataagat
     6001 tgagagcctc cgccgatcca tgtcttgcct gtgagctgaa ctcgaagtgt ccttgacatc
     6061 gttccgagcg cgaaacataa tcctgccctc agctcaaacc cccaagcgca atatcaatcg
     6121 ccccttcagg ttgacggcct cacctagccg aaagaggaaa atgatgatga gggaacgtgt
     6181 atctactgac ggtcagagtt cctggggcgg gccgagcgcg cggcgcggcc gcctcggtca
     6241 tttaacgcgc ttcgcctgcg cgctcgccgc gtggggcgcg ctgcttcatg cggcgccggc
     6301 ggtcgccgcc gccacgctgc cgagcggctt caccgagacg caggtcgccg gcgggctttc
     6361 gtcgccgacc gcgatggcct ttgcgcccga cgggcgcctg ttcgtctgcc agcagggcgg
     6421 ccaactgcgc gtcatcaaaa acggcgtgct gctgccgacg ccattcgtca cgctcagcgt
     6481 cagcgcctca ggtgagaggg gactgctcgg ggtggccttc gatccgaact tcgcctccaa
     6541 ccagttcgtc tacgtctact acaccgcgac gacgccggcc atccataacc gcgtcagccg
     6601 cttcacggcc gcgggcgacg tggccctcgg cggcagcgag accatcctcc tcgaacttga
     6661 taacctgtcg gccgccacga accacaacgg cggggcgatg cacttcggcg aggacggcaa
     6721 gctctacatc gccgtcggcg agaacgccaa cggggcgaac tcgcaaaccc tctcgaacct
     6781 cctcggcaag atgctccgca tcaactccga cggcaccatc cccgccgaca accccttctt
     6841 cacacaggcc gtgggcaaaa accgcgcgat ctgggcgctg ggcctgcgca acccctacac
     6901 cttcgcgttc cagccggtga cgcgccgcat gttcatcaac gatgtggggc agaacacctt
     6961 cgaggaggtc aacgaaggtc tcgtcggggc gaactacggc tggcccgaca cggaggggcc
     7021 gacgacgaac cccaacttcc gcacgccttt ctattactac ggccacggcg gcggcctctt
     7081 cgggggctgc gccatcaccg gcggcacgtt ctacaacccg tccacgcaac agttccccgt
     7141 cgagtacggg ggcgattact tcttcgccga ctactgcggc ggctggatca actacctgac
     7201 cgacccatcg aacaccaacg ccgtcgcgaa cttcgcctcg ggcatctcct tccccgtaga
     7261 cttacaggtc ggccccgagg gcgcgctcta ctacctcgcc cgcggcgcgt ccgccggcgc
     7321 cggcgtggtc ttccgtgtcg aatacacggc caaccaggcg cccgccatca cacagcaccc
     7381 gtcgaaccag acggcctccg cggggcagac ggccaccttc agcgtcgcag ccagcggcac
     7441 tggcccgctc tcctaccagt ggcagaggga cggcgttgac atcaacggcg cgaactcgtc
     7501 cgcctacacg accccgcccg tctcggcggc cgacaacggc gcacgcttcc gctgcctcgt
     7561 ctcgaacgcc tttggcagtg ccaccagcaa cgaggccgtg ctcaccgtcg tctccaaccg
     7621 cccgcccgtc gccaccatca cgcagcccgc ggccggcgcg ctctacagcg gcggcgacac
     7681 gatcaactac gcgggcaccg gcaccgaccc ggaggacggc acacgcccgg cgagcgcctt
     7741 cacgtgggaa gtggtcttcc accacgacac ccacacgcac ccgttcatcc cgcccacttc
     7801 cggcgcgacg ggcggctcct tcaccatccc gcgcacgggc gagacttcgg ccaacgtctt
     7861 ctaccgcatt cacctgacgg tcgccgactc cggcgggctg acgcacacga ccttccgcga
     7921 cgtgcgcccc cgcacggtga cgatcacact ggcggcctcg ccctcggggc cacaactcac
     7981 acttgacggc cagccggtgg cgacgccaca ttcattcgtc ggcgtggtcg ggatgacgcg
     8041 caccatcgcc gcgccctcgc agtccgtcgg caacttctcc tacaccttcc gctcgtggtc
     8101 ggacggcggc gcgcagaccc acaccatcac gacgccgtcg gtcaacacca cctacacggc
     8161 gtcgttcagg aagaagaagc agcagtaggg cacgcggcaa cgagttaccg acttcgggag
     8221 gaagatagaa ggaacacgga tgagacggat ggagcggatt tacacggatt tgatccgtgc
     8281 ttatccgtct tatccgtctc atccgtgttc cgtgttctga cgcctcaaag gacgccggcg
     8341 cacgtcgctc ggcagggtca ctgactgttg agaaattgca ggtcgagctt ctcgccgcgc
     8401 acttcaatcc gcaccgtccc gccggtctga agactgccga agagaatctg ctcggcgacg
     8461 ggcgctttga tctcggtctg gatgaggcgg gacatggggc gggcgccgaa ctgtttgtcg
     8521 tagccgtggg tggcgagcca gcggcgcgct ccgtcggtga gttcgatgga gacgttcttg
     8581 gcggagagct gcgcgccaag ctcgccgatg aatttatcga cgacgcgctc ggtggtctcg
     8641 aacgagaggg cgtcgaaggc gatccaggcg tcgaggcggt tgcggaactc ggggctgaag
     8701 gtgcgctcga tgacgccgcg gcccttgccg cgacccgcac cgccgccctc gccgtgctgg
     8761 aagccgatct tcgcgtcgct catctcgcgg gcgccggcgt t
//
LOCUS       DASVES010000205         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_109292, whole genome shotgun sequence.
ACCESSION   DASVES010000205 DASVES010000000
VERSION     DASVES010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109292"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VF240_08080"
     CDS             <1..867
                     /locus_tag="VF240_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7175227.1"
                     /translation="FVRGIYGIQPAELGVEAAFPTLSVAPGRTLLGTMFGKALKRPPK
                     NSNGSNGGGAKKQGRERRRMVAPRFGMGDLTGRLERRLEERLGGRFRRGVRVEEVPEG
                     PNVILATPAYAAARLLEPLAPELSRLLLEVRYTPIVSVTAFVPVDSLTREVKGVGALV
                     PAREGRKSLGILFTSSSFEGRVTDDGRYASFSVLLGGSSQPHWVNATDAEIERAVRDE
                     LEDLLGVRGAPARLVISRWGRAIPQYSVTLPAVWQKARETWCATPGRLLFGNYTGQVS
                     LRGMIERAATAG"
     gene            complement(1196..1957)
                     /locus_tag="VF240_08085"
     CDS             complement(1196..1957)
                     /locus_tag="VF240_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175228.1"
                     /translation="MELAEMGSVRWEDVDDVEGVARLVALQDPEGGRLLEECWPEVMS
                     LEDMGLYGEAAYECRKMLEVSERPQVYRLVALEHVVCAERARAVLLGRECVDDDPSDP
                     DRHRVLGDVHFDLECYGDAARAYQRAVELGAGDAGLLLSLGLCHALLGDYGRAAVWHG
                     RALAADPESEDSLHEISWAYHRLGCSSKAIAVLERSLALEADPGCAECRGRLAATYRA
                     HGRHAEAEAADRQPARPWGRRRSEQKVTAGRGRVP"
     gene            complement(2569..2645)
                     /locus_tag="VF240_08090"
     tRNA            complement(2569..2645)
                     /locus_tag="VF240_08090"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2609..2611),aa:Asp,seq:gtc)
     gene            complement(2706..2780)
                     /locus_tag="VF240_08095"
     tRNA            complement(2706..2780)
                     /locus_tag="VF240_08095"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2746..2748),aa:Val,seq:tac)
     gene            complement(2961..3161)
                     /locus_tag="VF240_08100"
     CDS             complement(2961..3161)
                     /locus_tag="VF240_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175229.1"
                     /translation="MSVIGRLDDQVDAVLIKPLSKKGERVDAHEDAPAQTSTPRPADA
                     APAADDATDRERPPRELPVWLL"
     gene            3244..>3705
                     /locus_tag="VF240_08105"
     CDS             3244..>3705
                     /locus_tag="VF240_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175230.1"
                     /translation="MSGSPARRRRAVASATLAAIVFVGALLALTAAPLSAGAHGARAP
                     RRQQPNSVQRPRRVGGATATPDPTAPSAPPSRATPSPRPDAGEEVGEDDVVRVDTQLV
                     SVPTVVTDRAGRPVAGLRADNFQVFEDGRPQQIVNFATTEAPFEVALLLDTS"
BASE COUNT          685 a         1218 c         1164 g          638 t
ORIGIN      
        1 ttcgtccgcg gcatctacgg tatccagccc gccgaactcg gagtcgaggc cgctttcccc
       61 acgctctccg tcgcgcccgg ccgcacgctc ctcggcacga tgttcggcaa ggcactcaaa
      121 cgacccccga agaacagcaa cggctctaat ggcggcggcg cgaagaaaca agggagggag
      181 cgcaggcgca tggttgcgcc gcgcttcggc atgggagacc tgacggggcg gctcgaaagg
      241 cgtctggagg agcggctggg cgggcgcttc cgccgcggtg tacgcgtcga agaagtcccc
      301 gaggggccga acgtcattct cgcgaccccg gcttacgcgg cggcgaggct gctcgaacct
      361 ttagcgcccg aactcagtcg cctgctcctc gaagtcaggt acaccccaat cgtctcggtc
      421 acggccttcg tccccgtaga ctccctcacg cgcgaagtca agggtgtcgg cgcgctcgtg
      481 cccgcgaggg aggggcgcaa atccttaggc atccttttca cctccagctc gttcgagggg
      541 cgcgtcacag acgacgggcg ttacgcctcc ttctccgtac ttctcggcgg ctcttcgcag
      601 ccccactggg taaatgccac cgacgcggag atcgagcggg ccgtgcgcga cgaactagaa
      661 gacctgctcg gcgtgcgcgg cgcgcccgca cgcctcgtca tcagccgctg ggggcgcgcc
      721 atcccgcagt actctgtcac gctcccggcg gtctggcaga aggcgcgcga gacgtggtgc
      781 gccacgccgg gccgcctcct gttcgggaat tacacgggcc aggtcagcct gcgaggaatg
      841 atcgaacggg cggccaccgc cggctgaatt tcacttcagg cgcatggtcg agtcggcgtg
      901 gttcccaaat cacccgtatt gacgacgcaa agataggctc cagcaccacg cgttccatgc
      961 atcttgtggg cgcgggagga taacggtggg cgtgaggcga ctatatagat tgggtaagtg
     1021 tcaggtgagg actatgaaat gcactcagaa cagactgtaa tgaccgggaa ggagctcgct
     1081 cctagaaaga acatcatcac ggcctcgatg agttcgatcg cgctggctgt ccatcgagtt
     1141 ccttaaccag ccggtggata ggaatagtgc tacgcgtgcc gacgaacggc tcccgtcacg
     1201 gcacgcggcc cctcccggcc gtcaccttct gctcagaccg ccgcctgccc cagggtcgcg
     1261 ccggctgcct gtccgccgcc tcggcctccg cgtgcctgcc gtgcgccctg taggtcgcgg
     1321 cgagcctgcc ccggcactcc gcgcacccag ggtcggcttc gagtgcgagt gagcgctcga
     1381 gtaccgctat cgccttagag ctgcatccga gacggtggta ggcccaactg atctcgtgga
     1441 gagagtcctc cgactccggg tcggcggcga gagcgcgccc gtgccacacg gccgcacgcc
     1501 cgtagtcgcc gagcagggcg tggcacagcc cgagagagag tagcaagccg gcgtcgccgg
     1561 ccccgagttc caccgccctc tggtaggccc tggcagcatc cccgtagcac tccaggtcaa
     1621 agtggacgtc gccgagcacg cggtggcggt cggggtctga cggatcgtcg tcgacgcact
     1681 cgcggccgag gaggacggcc cgcgcacgct cagcgcagac gacgtgctcg agcgcgacga
     1741 gccggtagac ctgcggccgc tcggagactt caagcatctt gcggcactcg taggccgcct
     1801 cgccgtagag gcccatgtcc tcaaggctca tcacctccgg ccaacactct tcaagcagcc
     1861 gcccgccctc cggatcctgt agcgccacca ggcgcgcgac gccctcgacg tcatcgacgt
     1921 cctcccaccg aacgcttccc atctcagcca attccatact ttgtctcctt cagtcatagg
     1981 ctcaaccaag cggcacgaag ggtgcgcgcc acgttattag gagaaccgac gggcgctgac
     2041 gagtcaaggc catcgtccgg ttaactcaca actctgaaac gatgaatagt ttacgaaccg
     2101 ccatggcggt tcacgtttta attgtttcat gaattcgtgc gtgacaaagt taggacagta
     2161 gtgtggtcat gtagcgtgaa aaaggcgtaa gcacttgcac ctctcagcgg gcgcatcccg
     2221 taatagatgt cagcgagcgg taacaagaat gaggtagtgc ggggcaccga actttgcatt
     2281 ccttccccaa ggtctgggag ccgggtagcc agacaatacc tcccacaaga acctaagaaa
     2341 ttttgaattc gacagtgagc ctaccgattt tctaaccgag gacgaagcag acaggaatat
     2401 gcctcccaca cagcggcggg aattttgacg ggactcaagg aagtagagag gacgtaatac
     2461 cttcagtctg gcgcacaagc ccaagacttt tacccctgga gaatggctgc aagatcgcgg
     2521 taaaagtaaa taagcgcgcg cctgacgaat caggcgcgcg cttaattttg gcggagccga
     2581 cgggactcga acccgcgacc tccgacgtga caggccggcg ttctaaccaa ctgaactacg
     2641 gctccgcacg ggaacctgaa attttcaaac gcggcgtgcg ccccttcggt cggccgccgc
     2701 tcatctggta ggcacggagg gattcgaacc cccaacctcc accttgtaag ggtggccgtc
     2761 taccgttgac atacgtgcct tcaaaaaaac tgccgcccgg ctagcgtctc aaacttcgta
     2821 agtgtcgagc gtgatttcaa tttacgaaat ggccgcgcgg cgtgtcaagc gcatgtgccg
     2881 acgagacgcc gatcaattag acgatgatca gttaaaggaa ccgccccagg agttgcgcgt
     2941 gaaggtcggg gcgcgcgggc ctacaacaac cagaccggca actctcgcgg cgggcgttct
     3001 cggtcggtcg cgtcgtccgc agccggcgcg gcgtcggccg gtcggggcgt ggaggtttgc
     3061 gccggggcgt cttcatgggc gtccacacgc tcaccttttt tcgagagcgg cttgatgagc
     3121 acggcgtcca cctgatcgtc gagtcgtccg atgacactca tgtgcgcgtt tgcctccttt
     3181 ccgtcgcggc gcgtttgtgg ctatattgag cgcgtgaact gctttaccgg ggagtgtaag
     3241 acggtgtcgg gtagtccggc aagaaggcgg cgggccgtag cgtcggcgac gctcgcggca
     3301 attgttttcg tcggcgcgct gctcgccctg acggccgcgc cgctctcggc cggcgcgcat
     3361 ggagcgcgcg cgccgaggcg acagcagccg aactccgtgc agcgtccgcg ccgcgtgggc
     3421 ggcgccaccg cgacgcccga cccgaccgca ccctctgctc cgccatcgcg cgcgacgcct
     3481 tcgccccgac ccgacgccgg cgaggaagtt ggcgaagacg acgtggtgcg cgtggacacg
     3541 cagctcgtct cggtgcccac ggtcgtcacc gaccgtgcgg gccgccccgt cgcgggtctg
     3601 cgcgccgaca actttcaggt cttcgaggac ggacgcccgc agcagatcgt caacttcgcg
     3661 accaccgagg cgcccttcga ggtggcgctg ctcttagaca cctcc
//
LOCUS       DASVES010000206         6162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_784150, whole genome shotgun sequence.
ACCESSION   DASVES010000206 DASVES010000000
VERSION     DASVES010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784150"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(732..2702)
                     /locus_tag="VF240_08110"
     CDS             complement(732..2702)
                     /locus_tag="VF240_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7175231.1"
                     /translation="MSAFEMKTFPSYAFGPFVLNGSERLLLRGNEVVALTPKVLETLF
                     VLVENHGRVLTKNVLMEALWPDAFVEESSLSQNISLLRKALGNGQADQVYIETLPKRG
                     YRFVADVREINAEPGSMKSGLDAPLIQSDAANSEGVEAPDEERVKPFGLPTVRDIRPR
                     LKWQTLGVLVCLIAVSAGMFYWWRIRTAKRQVGMALKSVAVLPFKTVGGAAETELLGL
                     GMADSVILKLTRFERLRVLPTSSVFKYLGAEKDALSIGRELGVDAVLDGTVQRWGDQV
                     RVTAQLITLKDGKTLWSGKFDEQYRDIFCLQDSVSELLAGDLRLQVLNLQRKAEARPI
                     TKDPEAYQAYLTGLYFWNQRSSESLPKAIDHLQRAVSEDGNFALAYALLADCHYVSLG
                     MLKGDQQGATTAMQKAEAAVARAIELDETLAEAHTAMGGLLSHKGDGDGASKAYRRAL
                     ALNPNYATARVRYAYFLFTLLDPDGALREARLAQALDPMSSVTNAALGYMLMMRREWE
                     ESIPYFERSLELNPEATGSRIKLGLAHLHCGRFEEAVAEFSTVANRDPISSQRHLALT
                     YARLGRRDEAHKLLDEILRSPSRNQSSNYDLAIIYFAMGQKERAFELLRKSTLSWTER
                     ALLKLDPELDNVRSEPRFVALINKQSYPGQSS"
     gene            complement(2873..3697)
                     /gene="rsmA"
                     /locus_tag="VF240_08115"
     CDS             complement(2873..3697)
                     /gene="rsmA"
                     /locus_tag="VF240_08115"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7175232.1"
                     /translation="MYPKAKKRLGQNFLVDGGVAERIVRALGPGADETVVEIGPGRGA
                     LTERLLERAGRVVALEFDRDLVAYLRGRFGAHDNFTLVQADALNADFCELVAPAARAR
                     VVANLPYNISTAILQRLISHRECVSEMVLMLQREVVERITAGPGDAERGYLSVMVENY
                     CEAEGLFDVPPGAFRPVPKVWSTVARLRMRPSPAVVVRDEKLLWRTVSAGFAQRRKTI
                     NNNLRGAPPDLLARVEAAGGASAVLAAAGVEPGRRAETLALGEWGRLADALEAGSR"
     gene            complement(3813..4934)
                     /gene="ribD"
                     /locus_tag="VF240_08120"
     CDS             complement(3813..4934)
                     /gene="ribD"
                     /locus_tag="VF240_08120"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX7175233.1"
                     /translation="MNFELSEIDERLMRRALELAARGEGRVSPSPLVGCVVANADGEV
                     VGEGFYLYDGLKHAETLALEQAGGRARGATAYVSLEPHAHTGRTPPCTSALIEAGVSR
                     VVASIEDPNPLVSGRGFEHLRRAGVEVVTGVLAAESALLNEQYVHAMTHGRPFVHLKL
                     ACSLDGRIATRTGDARWITGEESRARVHELRHRYDAILVGAGTAVKDDPLLTDRSGLT
                     RRRPLVRVVLDERLQICADSKLARTASTEPLIVFTSAHADERKARELEARGVEVVRDA
                     GGWRAPAAVLEQLYRRSLQGVLIEGGAHVAGVFLEAGLVNKVSFFVAPLIIGGGEAKS
                     AVGGSGAITVDQAVALDAVEVVRRGRDVEITGYPKRRKS"
     gene            complement(5026..6012)
                     /gene="ftsY"
                     /locus_tag="VF240_08125"
     CDS             complement(5026..6012)
                     /gene="ftsY"
                     /locus_tag="VF240_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7175234.1"
                     /translation="MLGLNRSMEELEAEVEALEQAYSARFSRAISATRDSLSEKIDTV
                     FQNRKQIDAELLDELEEALIAADIGVPTTLEILDKVRRAVSRKEIDDIDALKAALKNE
                     LLTILKRSEAQGITDEASVAESVAPYVIMVVGVNGVGKTTTIGKLAQRIKAEGNDVLI
                     CAADTFRAAASEQLAIWAERTGVPLIQQKHGTDPAAVLFDALKAAKARASDVLIVDTA
                     GRLHNKSNLMAELEKMKRVAGREVEGAPHETLLVVDAVTGQNGLEQARQFLKVAGVTG
                     IVLTKLDGTAKGGIAVAISRELNLPIRYAGIGEKVDDLVIFDPEQYVNGLFA"
BASE COUNT         1166 a         1939 c         1805 g         1252 t
ORIGIN      
        1 gtgtgtcgca tcactgaagt agacatccct cagaaatctt gttgtcccac tggcttgccc
       61 cgaccaagtg tttccgccat tggttgtgac cctgatcact ccgcctgtcc cgacggcgta
      121 acccttctga ttatccgtga acgcgacacc gagcaggact gccgatgtgc cgctcgtttg
      181 agagatccat tctggagtcg tctggacgct gacacgactg ctgacgattc ccatcccgtg
      241 aaacggcccg actggaaggg tgacattcct cgcacttccc ccccgtattc cgaccgccat
      301 tagcgtcagc cctacaaggc ccacagttag acagactctc agccgtaaac ttgatttaca
      361 gaaggttgtt agcatggaaa ttccgatcct ttttggtttc ggggcgagaa gtggggagaa
      421 tatgttctta acgcgagata aaagaacccc gtcgttcggc acatcgtaaa cgtgccgagc
      481 gcgaaaagtc ctaaaagact tctgataaaa tcctgataga tttctatgag gtgatttaag
      541 aaggcgaaag aggctttaga gcgactttca cttgagtgaa agcgaacatt gattcaccgc
      601 agaggcgcaa tagaacgcgc cgcggtgaaa agctttcgct tttccggtgt ggttttctta
      661 attcttcttg accggggttg ggtcttagaa aagcataaga caggggagag acaccgtgct
      721 gttccgcaat actatgaact ctggccgggg taggattgct tgttaatgag cgccacgaac
      781 ctcggttctg atctgacgtt gtccagttca gggtcgagtt ttagcaaagc cctttccgtc
      841 cagctcagcg ttgactttcg caacagctcg aacgcccgtt ccttttgacc catggcgaag
      901 tagataatgg cgaggtcgta gttgctcgac tgattgcggc tcggtgatcg caaaatctca
      961 tctagcagct tgtgagcttc atctcgtcgc cccagcctcg cgtatgtcag cgccagatgc
     1021 ctctgagaag agatcgggtc tcgattggct accgtggaaa actccgccac ggcctcctcg
     1081 aaacgcccac agtggaggtg tgctagtcct aatttgatgc ggctcccggt tgcctccggg
     1141 ttgagttcga gtgaacgctc aaagtacggg atggattcct cccactcacg tctcatcatg
     1201 agcatgtacc ccaatgctgc gttagtgacc gacgacattg ggtcgagagc ctgagccagc
     1261 cgtgcctcgc ggagcgcccc atccggatca agaagtgtga ataaaaagta cgcgtacctg
     1321 acacgagcgg tagcataatt gggattgagt gcgagtgctc gtcggtatgc tttcgacgct
     1381 ccatccccgt cgcctttatg agacagcagc ccccccatag cggtgtgagc ctcggcaagt
     1441 gtctcatcaa gctcgatggc cctcgcgacc gcggcttccg ctttttgcat ggccgtcgtg
     1501 gcgccctgct gatcgccttt gagcatccca aggctgacgt aatgacaatc ggccagaagc
     1561 gcataggcca acgcaaaatt cccgtcctca ctgacggctc gctgcaagtg gtcgatagcc
     1621 ttcggcaacg actcacttga tcgttgattc cagaaatata gtccagtcaa gtacgcctga
     1681 taagcctccg ggtctttggt aatcggcctc gcttcggctt tccgttgcag gtttaagact
     1741 tgcagtctta aatcgcccgc taaaagctcg gagaccgaat cttgcagaca gaaaatatct
     1801 cggtattgct cgtcaaactt gccggaccag agcgtcttcc cgtctttgag agtaattaat
     1861 tgagcagtca cccggacctg gtcgccccaa cgttgaaccg tgccgtcgag gacagcgtca
     1921 acacccagtt cccgtcctat cgagagcgcg tctttttccg cgcccagata tttgaaaacc
     1981 gaactcgtcg gcagcactct gagtcgttca aatctggtaa gtttgaggat taccgagtca
     2041 gccatcccca accccagcag ttcggtttca gccgcgcccc ccacggtttt gaatggcaac
     2101 accgcgaccg actttaaggc cattcccact tgtctctttg ctgtgcggat gcgccaccaa
     2161 tagaacatgc cggctgatac ggcgatgaga caaacaagca ctcctaaagt ttgccacttc
     2221 aaacgggggc gaatgtcccg gactgtgggt aatccaaaag gcttcacccg ctcttcatcc
     2281 ggcgcctcga caccctccga attggccgca tcgctctgaa ttagcggcgc atccagacct
     2341 gacttcatcg agccgggttc agcgttaatt tcgcgcacgt cggcgacaaa gcggtagccg
     2401 cgctttggca gcgtttcgat atacacctga tcagcctggc cattgcccag agccttccgc
     2461 aacagcgaga tgttctggga caggctgctc tcctcaacga aagcatccgg ccagagggct
     2521 tccatcagga cgttcttggt gaggacccga ccgtggttct cgaccaggac aaacagggtt
     2581 tccaatactt ttggcgtcag cgcgacgact tcattgccac gcaatagcaa ccgctcggag
     2641 ccattgagaa caaatggccc aaacgcgtag gatggaaacg tcttcatctc gaaagcggac
     2701 ataaaactcc gtttctgcgt atcggcgcac gtccctgttg cagagtcgcc gcagggcgcg
     2761 gaggaggcgc cgcagacttg aatccactct actctctgcg tgaacttcgc gtccacttcg
     2821 actctgtgtt gaatccgacc tgctttaccc tcaactcaat atcgatttgg aattatcgcg
     2881 acccggcttc gagcgcgtcg gccaaacggc cccactctcc gagcgcgagc gtctcggcgc
     2941 ggcggcccgg ctcgaccccg gccgctgcga gcacggccga agccccgccc gccgcctcga
     3001 cgcgcgcgag caagtcgggc ggcgcgccgc gcaggttgtt attgatggtc ttgcgccgct
     3061 gcgcgaagcc cgcgctcacc gtccgccaca gcagcttctc gtctctgacg acaaccgccg
     3121 gactcggcct catgcgcaga cgcgccaccg tgctccaaac cttcggcact ggccggaacg
     3181 cgcccggcgg cacgtcgaac aatccttccg cctcgcaata gttctcgacc atcaccgaca
     3241 ggtatccgcg ctcggcgtcg cccggccccg ccgtaatgcg ttcgaccacc tcgcgctgca
     3301 acatcagcac catctcgctc acgcactcgc ggtgactgat gagacgctgg aggatggccg
     3361 tcgaaatgtt gtacggcaaa ttcgcgacga cgcgcgcccg cgccgccggg gcgaccaact
     3421 cgcagaaatc cgcgttgaga gcgtcggcct gaacgagagt gaagttgtca tgagcgccga
     3481 aacgaccgcg caggtatgcg acgaggtcgc ggtcgaactc cagcgccacg actcttcccg
     3541 cgcgttccag cagccgctcc gtcagcgcgc ccctccccgg cccgatctcc acgacggtct
     3601 cgtccgcgcc cggcccgagt gcgcggacga tgcgctcggc caccccgccg tcaacgagga
     3661 aattctgccc cagcctcttt ttcgccttcg gatacataag agccgtgaat cgtcaatcgt
     3721 caatcgtcaa tcgtggtcgg cttaacgcgg cgtgaaggat gaatcgcaag ttgagtaata
     3781 agcaatcgaa ttttcgattc acgattcacg atttacgatt tacgtctttt ggggtagccg
     3841 gtgatctcta cgtcgcggcc gcggcgcacg acttcgacgg cgtcgagcgc gacggcttga
     3901 tcgacggtga tggcgccgct gccgccgacg gccgacttgg cctcgccccc gccgatgatg
     3961 agcggggcga cgaagaagct caccttgttt accagccccg cttcgaggaa gacgccggcg
     4021 acgtgcgcgc cgccttcgat gaggacgccc tgcaaggagc gccggtaaag ttgttcgagg
     4081 acggccgcgg gcgcgcgcca cccgcccgcg tcgcgcacga cctccacgcc gcgcgcttca
     4141 agctcccgcg ccttacgctc gtcggcgtgc gcggaagtga agacgatgag cggttcagtt
     4201 gaagccgtcc gggcgagctt cgagtcggca cagatttgca ggcgctcgtc gaggacgacg
     4261 cggacgagcg ggcggcggcg cgtcaggccg ctgcggtcgg tgagcagtgg gtcgtccttc
     4321 acggccgtgc ccgcgccgac gagaatggcg tcgtagcggt ggcgcagctc gtgtacgcgc
     4381 gcgcgcgact cctcacccgt gatccagcgc gcgtcgcccg tgcgcgtggc gattctcccg
     4441 tccagagaac acgcgagctt caggtggacg aacgggcggc cgtgtgtcat ggcgtggacg
     4501 tactgctcgt tgaggagcgc cgactccgcg gccagaaccc cggtgacgac ttcgaccccg
     4561 gcgcgtcgca gatgctcgaa gccgcgcccc gacacgagcg ggttcgggtc ttcgattgag
     4621 gcgaccacgc ggctgacgcc ggcctcgatg agcgcgctcg tgcaaggcgg cgtgcgcccc
     4681 gtgtgcgcgt gcggctccaa cgagacgtaa gcagtcgcgc cccgcgcacg gcctcccgcc
     4741 tgttcgagcg caagcgtctc ggcgtgcttc agcccatcgt agaggtagaa gccctcgccg
     4801 accacttcgc cgtcagcgtt tgcgacgacg caccccacga gcggactcgg actcacgcgc
     4861 ccctcgccgc gcgcggcgag ttccagcgcg cggcgcatta ggcgctcgtc aatctctgaa
     4921 agttcaaagt tcaaagttca aagttcaaag ttcaaaggca aagctcaaag ttacaagggc
     4981 ggctccgact tcttcccttt gaactttgaa ctttgaactt tgaacttaag cgaagagtcc
     5041 gttgacgtac tgctccgggt cgaagatgac gaggtcgtcc actttctcgc cgatgccggc
     5101 gtagcggatg ggcaagttca gctcgcggga gatggcgacg gcgatgccgc ctttggccgt
     5161 cccgtcgagc ttcgtcagga cgatgccggt cacgccggcg accttcagga attgccgcgc
     5221 ctgctccagc ccgttctggc ctgtgacggc gtccacgacg agcagcgtct cgtgcggcgc
     5281 gccctccacc tctcgtcccg ccacgcgctt catcttttcg agttcggcca tcaggttcga
     5341 cttgttgtgc aagcgcccgg ccgtatccac gatgagcacg tcggaggcgc gggccttcgc
     5401 cgccttgagc gcgtcgaaga gaacggccgc cgggtcggtg ccgtgcttct gctggatcag
     5461 gggcacgccg gtgcgctcgg cccagatggc gagctgttcg gaggcggcgg cgcggaaggt
     5521 gtcggccgca cagatgagca cgtcgttgcc ctcggccttg atgcgttgcg cgagcttgcc
     5581 gatggtcgtg gtcttcccta ccccgttcac gccgacgacc atgatgacgt aaggcgcgac
     5641 cgactccgcg acgctcgcct cgtccgtgat gccctgcgcc tctgagcgct tgaggatggt
     5701 gagcagttcg tttttaaggg ccgccttgag cgcgtcgatg tcgtcgatct ccttgcggga
     5761 gacggcgcgg cgcaccttgt cgagaatttc gagcgtggtc ggcacgccga tgtcggcggc
     5821 gatgagcgcc tcttcgagtt cgtcgaggag ttcggcgtcg atctgtttgc gattctggaa
     5881 gaccgtgtcg atcttctcgg agagcgaatc gcgcgtggcg gagatggcgc gcgagaaccg
     5941 cgcggagtag gcctgctcaa gcgcctccac ctccgcctcc agctcctcca tcgaacggtt
     6001 gagaccgagc accgaagtcc cgaaagacga gcgcgacggc gcgggacgtt gcgccggggc
     6061 agttgccgct tccgcgcgcg ccgaatcatt cgtccttacc tgtgttggtg gcttcagagt
     6121 tgaggcagat gccgttttaa aagactcgac tgccggcgtc tt
//
LOCUS       DASVES010000207         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_195932, whole genome shotgun sequence.
ACCESSION   DASVES010000207 DASVES010000000
VERSION     DASVES010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195932"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VF240_08130"
     CDS             complement(<1..185)
                     /locus_tag="VF240_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175235.1"
                     /translation="MKHNSSIVVEESHAAAGREAVASLKSVSTLYLCYFGLREPLVQT
                     QVLPYLRQLVAAGAVVHL"
     gene            complement(419..1465)
                     /locus_tag="VF240_08135"
     CDS             complement(419..1465)
                     /locus_tag="VF240_08135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175236.1"
                     /translation="MTRAADGAAVKSQMKISVIIPARDEERSITALLDALLAQTRAPD
                     EIVVCDNGSTDRTAEIVEGYARRGASVQLIRAGAGFPGRGRNVAAAGASHEWLAFIDA
                     GIRPERDWLEHLAARARSEPEADVVYGNWEPVTDSLFTQCAAMAYLPPPAEVEGRWLR
                     ARFIASSLVRRAVWGAVGGFPEHLRSAEDILFMDELERAGYAATYEPRAVVRWDIQPN
                     LWRTLRRFTSYSRHNLRAGLWRQWHAPVLRRYALLAALLLPALLVGAWWLAVVAGLWL
                     LMLTARAGVALRRNRHIYPASAGRQLLRLCVLVAIIATLDAATIAGSIDWLFRDKLRG
                     DAASAAKGTNERSV"
     gene            complement(1462..2718)
                     /locus_tag="VF240_08140"
     CDS             complement(1462..2718)
                     /locus_tag="VF240_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175237.1"
                     /translation="MTANETDPRSARPAPATNAARRRRRLQRVLLLCVSSLVALGLAE
                     AVLRQVEKSSFGDHATTEETFLTDAVLKHRVKPFTLGHDARGFRNDSVPERADVVALG
                     DSQTWGVNVEREQAWPQELGRLTNRRVYNMSLGGYGPVQYWTLTDEALTLRPRTLVVG
                     LYFGNDLYDAYAMAYQYDAHAPLRRPDAPADIADDTVGRRAEEYWNHERAFTYDYGAR
                     GAGGWDDWLRGHTAIGRRLDRAGLWPSPVNLQHKIGRAWAERHPERGAVFEGEGAART
                     VFTIAYRQAAVDLSEPRIVEGLRITKELLTRINVKAEASGARLLVLLIPTKESAYADV
                     LRERGARLHETHEKLIRQEAAARAEIQAMCERERIVCADALPRLSEAVRSGARVYSQS
                     AESHPHASGYALISAVVCDELARRGW"
     gene            complement(2832..>3342)
                     /locus_tag="VF240_08145"
     CDS             complement(2832..>3342)
                     /locus_tag="VF240_08145"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7175238.1"
                     /translation="VMNLKIKYRESFRPFAPSVLRENVSEWFDLDTDSPYMLLVASVR
                     EDRRRRMTEGEERLWGIERLNVARSEIPAVTHVDYSARVQTVRRETNPLYWEIIEAFR
                     QRTGCPVVVNTSFNVRGEPIVCTPADSYRCFMRTEMDALVLENFILDKAGQPAFADRA
                     DWRKEFALD"
BASE COUNT          543 a         1107 c         1147 g          545 t
ORIGIN      
        1 aggtgaacga cggcgccggc cgcgacgagc tgtcgcaggt agggcagcac ctgcgtctgc
       61 acgagcggct cgcgcagacc gaagtaacag aggtagagcg tgctgacgga cttcagcgac
      121 gcgacggcct cgcggcccgc cgccgcgtga gactcttcaa caacgattga agaattatgt
      181 ttcatccgcc gatgactatc agtcgtcgcc accccgccga acaatgttga taaaactgta
      241 ggggcagggc ttgtccgcgg cccgtggcgc caaaggaatt aacatggatg gacaggatgg
      301 gcaggacgaa gaacagattt gagatttgag atttcaaatc ttctcatcct atccatcctg
      361 tccatccatg ttaataattc tgaaaactag ggcagcggac ttggccctgc ccctacaatt
      421 ataccgaacg ctcgtttgtt cccttcgccg ccgacgccgc gtcgccgcgc agcttgtctc
      481 ggaacagcca gtcgatgctg cccgcgatgg tcgccgcgtc gagcgtggcg atgatggcga
      541 cgagcacgca caggcggagc agttgccgcc ccgcgctcgc gggatagatg tgtcgattgc
      601 ggcggagcgc tacgcccgcg cgcgccgtga gcatgagcag ccacagcccc gcgactacgg
      661 cgagccacca cgcgccgacg agcagcgcgg gcagcaggag cgcggcgagg agcgcgtagc
      721 ggcgcagcac gggcgcgtgc cactgccgcc agagtccggc gcgcaggttg tgacgcgagt
      781 aggaggtgaa gcggcgcagc gtgcgccaca ggttgggctg gatgtcccag cgcaccacgg
      841 cgcgcggctc atacgtggcc gcgtagccgg cgcgctccaa ctcgtccatg aacaggatgt
      901 cttcggccga gcgcaggtgc tcggggaagc cgcccaccgc gccccagacc gcgcgccgca
      961 ccagactcga cgcgatgaag cgcgcccgca accatcggcc ttcgacctcg gccggcggag
     1021 gcaggtaggc cattgccgca cactgcgtga ataagctgtc cgtgaccggc tcccagttgc
     1081 cgtagaccac gtcggcctcc ggctcgcttc gggcgcgggc ggcgaggtgt tcgagccagt
     1141 cgcgttcggg gcgaatgccg gcgtcgatga aggcgagcca ctcgtgcgag gcgccggccg
     1201 ccgcgacgtt gcggccgcga ccgggaaagc ccgcgcccgc gcggatgagt tggacgctcg
     1261 cgccccggcg cgcatagccc tcgacgatct cggccgtgcg gtcggtcgag ccgttgtcgc
     1321 agacgacgat ttcgtcgggc gcgcgcgtct gtgcgagcag cgcgtcgagc agcgccgtga
     1381 tgctgcgctc ctcgtcgcgc gcagggatga tgacgctgat cttcatctga ctcttgacgg
     1441 ccgcgccgtc ggccgcgcgc gtcaccagcc gcgcctcgcc agctcgtcac acacgacggc
     1501 cgatatcagt gcgtagccgc tcgcgtgcgg gtggctctcc gcgctctgcg agtagacccg
     1561 cgcgccgctc ctgacggcct cgctcagacg cgggagcgcg tcggcgcaca cgatccgctc
     1621 gcgctcgcac atggcctgga tttccgcgcg cgccgcggcc tcctgccgga tcagcttctc
     1681 gtgcgtctcg tgcagacgcg cgccgcgctc gcgcagcacg tcggcgtagg ccgactcctt
     1741 cgtcgggata agcaggacga gcaggcgcgc gcccgacgct tcggccttca cattgatgcg
     1801 tgtcagcaac tccttcgtga tccgcaagcc ctcgacgatg cgcggctccg agaggtctac
     1861 ggccgcctgc cggtaggcaa tggtaaagac ggtgcgcgcc gccccttcgc cctcgaagac
     1921 ggcgccgcgc tcagggtgac gctcggccca cgcccggccg atcttgtgtt ggaggttgac
     1981 cggggagggc cacagccccg cgcggtcgag gcgtcggcca atggccgtgt gcccgcgcag
     2041 ccagtcgtcc cagccgcccg cgccgcgggc gccgtagtca taggtgaagg cgcgctcatg
     2101 gttccagtac tcctcggcgc ggcggccgac agtgtcgtcg gcgatgtcgg ccggcgcgtc
     2161 ggggcggcgc agcggcgcgt gggcgtcgta ctggtaggcc atcgcgtagg cgtcgtagag
     2221 gtcgttgccg aagtagaggc cgacgacgag cgtgcgcggc cgaagcgtga gcgcctcgtc
     2281 cgtgagcgtc cagtactgga cggggccgta gccgccgagg ctcatgttgt agacgcgccg
     2341 gttcgtgagc cggcccaact cctgcggcca cgcctgctcg cgctcgacgt tgacgcccca
     2401 cgtctgcgaa tcgccgaggg cgaccacgtc ggcgcgctcg gggacggagt cgttgcggaa
     2461 gcccctggcg tcgtggccga gggtgaacgg cttgacccga tgcttgagca cggcgtcagt
     2521 gaggaacgtc tcttccgtcg tcgcgtggtc gccgaacgaa gacttctcga cctgccgcaa
     2581 gaccgcctcg gccagcccca gagcgacgag cgacgagacg cacaggagaa gcacccgttg
     2641 gagacgccgg cgacgcctcg cggcgttagt cgccggggcg ggccgcgcgc ttcgggggtc
     2701 agtttcgttg gccgtcatta tgaaaaggaa atagtaaaaa ggtaaaagga agaagtggaa
     2761 aggcaagtgt gctcgtgctc tcttaacttt tgccttttgc ctttttactt ttgccgttcg
     2821 cctcgccccg gtcagtcgag cgcgaactcc ttgcgccagt cggcgcggtc tgcaaacgcc
     2881 ggctggccgg ctttgtcgag gatgaagttt tcgaggacga gcgcgtccat ctccgtccgc
     2941 atgaagcagc ggtagctgtc ggcgggcgtg cagactatcg gctcgccgcg cacgttgaag
     3001 gaggtgttga cgacgacggg gcagccggtg cgctggcgga acgcctcgat gatctcccag
     3061 tagagcgggt tcgtttcgcg ccgcacggtc tggacgcgcg ccgagtaatc gacgtgcgtc
     3121 acggccggaa tctccgagcg ggcgacgttc aaccgctcga tgccccacag acgctcctcg
     3181 ccttcggtca tccgccgccg acggtcttcg cgcacactcg cgacgagcag catgtatggg
     3241 ctgtcggtgt cgaggtcgaa ccactccgac acgttctcgc gcagcaccga aggagcgaac
     3301 ggccgaaacg attcgcgata tttgatcttg aggttcatca cc
//
LOCUS       DASVES010000208         6304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_966505, whole genome shotgun sequence.
ACCESSION   DASVES010000208 DASVES010000000
VERSION     DASVES010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966505"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VF240_08150"
     CDS             complement(<1..446)
                     /locus_tag="VF240_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175239.1"
                     /translation="MSMNPMGQLGQAAGAAQAQAAQATQAAQAAVAQTTQDALQQAQQ
                     AAGAVAQQAQGAVEQAAAQVNQATQQAAQQLRQTTDQALQQVQQAAQQGAQAAQQAVQ
                     QAQQQVSSAVDEAAQQVQQAAQQAAQQVQQQAQQAVQQAQQAGAMAA"
     gene            complement(443..877)
                     /locus_tag="VF240_08155"
     CDS             complement(443..877)
                     /locus_tag="VF240_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175240.1"
                     /translation="MLKIRSEQFEAFKPVAEDGFVRRLAEHVRARHPRVVVRLPERLT
                     VAGRLDDETLRALVRGGVARARRYGMEAESALAAFVVTMFLTAPNFDEHPLIRRVLAD
                     DKIPPDGKIDQLLQSVTAKNLKVVKDTYNPAAWHEASEGGAA"
     gene            complement(942..1442)
                     /locus_tag="VF240_08160"
     CDS             complement(942..1442)
                     /locus_tag="VF240_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEX7175241.1"
                     /translation="MPTGPAARITDNVVHPLPPVLTPGPGSPNVLIGALPAWRGVNAA
                     AAAGLQAAKQVSDAAIKTLEAATVAAAPTPGAPAAKAAEETGKATAATTMGTAITGAA
                     GGADIHSCMTLLPVPPHGPGVVIDGSKTVLINNLPACRLGDTVLEAVGPPNKIVRGEM
                     TVIIGG"
     gene            complement(1610..2149)
                     /locus_tag="VF240_08165"
     CDS             complement(1610..2149)
                     /locus_tag="VF240_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HEX7175242.1"
                     /translation="METEEITEALDAALFTEDGANAFAVIDGASVAGLLDKLYGLQPE
                     FACLYRGELAPDMAEVAPYLVRLEPATEFADWLVSEGWGKHWCVFALAHTGFREMRQH
                     FRSFLTVYDPEGKPLLFRYYDPRVLRVYLPTCDARELETIFGPVRAYLLEGEDPHEMF
                     RFELSSGALQQRTQPLAPG"
     gene            complement(2189..4276)
                     /gene="tssI"
                     /locus_tag="VF240_08170"
     CDS             complement(2189..4276)
                     /gene="tssI"
                     /locus_tag="VF240_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEX7175243.1"
                     /translation="MPTKFTQENRRIQIFTPLGEDVLLLRGFEGSEGVSSLFRFDLRL
                     LSENRSLAFDALVGKNATVKVSLADGSVRHINGICSRVSQGDTTSYAQEGTTGHLTEY
                     RATLVPWLWLLTRTSDCRIFQNLSAPDIIQKVFQDYGFTDFSNRLHGSYDVREYCVQY
                     RETAFDFVSRLMEYEGIFYFFEHEADKHTLVLADAPNEFKPCPNLPAAFYTSAIGEDA
                     AREAVKEWQKVQEVRPGKYTANDFDFQKPALDLTASVEGRDTRHYEIYDYPGGYVTRD
                     QGERLVGVRVQEQDSPLVVARGASSYAALASGYRVDLKGHYRRDMNGPYVLTSVQHSA
                     DLAAGYGSGAEGASEAIDGYSNTFECIPHPTPYRPPRVTPTPVIQGTQTAVIVGPAGE
                     EIYTDKYGRVKVQFHWDREGKNDENSSCWVRVSQNWAGKRWGAMFVPRIGQEVIVDFL
                     EGDPDRPIITGRLYNGGAMPPHALPDAKMKSTIKTYSTKGGGGFNEMSFDDTKGKEKV
                     FHHAEKDLHGRVKNDRVEWIGRDTHLIVKRDQLEQVEGDKHLKVTGDQNEQVDGGYSL
                     KVGQDAQTKAGTKFNVDAGQEIHLKAGMNVTIEAGTALTIKVGGNFININPGGIFIKG
                     TMVMINSGGSGGSGSGASPTAPTAPLEADTGEPGQAHEMPPARQPGVPVVYSPAALVM
                     RQAAQNGAPFCDI"
     gene            complement(4420..>6304)
                     /gene="tssH"
                     /locus_tag="VF240_08175"
     CDS             complement(4420..>6304)
                     /gene="tssH"
                     /locus_tag="VF240_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX7175244.1"
                     /translation="NRLKGVIEEVKSSPQPIILFIDEAHTMIGAGGAAGQNDAANLLK
                     PALARGELRTIAATTWAEYKKYFEKDPALTRRFQVVKVEEPTEDKAIVMMRAFAGYLE
                     NHHKVRVLEEAIEDSVRLSHRYISDRQLPDKCVSVLDTACAKVAIGQGATPPPVEDAQ
                     RRIDQIGTELRSLERESITGTDHAKRVDELRAALTEQEERLARLREQWDKERGIVERI
                     REVRTQLEQAVGGSNGGKPEVATAAAMAGNMEAATAAALTPAVPVDTEGLRAELGKLN
                     DELSQVQGETPLMQATVNSQAIAEVISGWTGIPVGRMMADEINTVLRLKDILEQRVIG
                     QSHALDSIAQRIRTSRANLTDPRRPVGVFLLVGPSGVGKTETALTLAETLYGGERNLV
                     TINMSEYQESHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVME
                     LFYQVFDKGMLEDGEGREIDFKNTVILLTSNVATDTIMNLCADPDTRPEPEPLVEAIR
                     PELIKVFKPALLGRMFVVPYYPISDEAMREIIKLKLGQVGRRMSENHDAEFDYDERVI
                     EEIANRCKEVESGARNVDHILTRTLLPEMSGEFLSRMAAGHPVARVHIGVGDGGQFVY
                     NIT"
BASE COUNT          897 a         2062 c         2145 g         1200 t
ORIGIN      
        1 gcggccatcg cgccggcctg ctgcgcctgt tggacggcct gttgtgcctg ctgctgcacc
       61 tgttgcgccg cctgctgcgc cgcctgctga acttgctgag cggcctcatc gacggcgctg
      121 ctcacctgtt gctgcgcctg ctgcacggcc tgttgtgccg cctgcgcccc ctgctgcgcc
      181 gcctgttgta cttgctggag agcctgatcg gtggtctgac ggagttgctg cgcggcctgc
      241 tgggtcgcct gattcacttg agcggcggcc tgctcgaccg cgccctgcgc ctgttgggcg
      301 accgcgccgg cggcctgttg cgcctgctgg agtgcgtcct gcgtcgtctg cgcgaccgcg
      361 gcttgcgcgg cctgtgtggc ctgcgccgcc tgcgcctgcg ccgcgccggc cgcctgtccg
      421 agttggccca tggggttcat gctcacgcgg cgccgccctc cgatgcctca tgccaggccg
      481 cgggattgta ggtgtccttg acgaccttca ggtttttggc cgtgaccgac tggagaagtt
      541 gatcgatttt tccgtcgggc ggaattttat cgtccgcgag gacgcggcgg atgagcgggt
      601 gctcgtcgaa gttcggcgcg gtcagaaaca tcgtcacgac gaaggcggcg agcgccgact
      661 cagcctccat cccgtagcga cgcgcgcgcg cgacgccgcc gcgcacgagc gcgcggagcg
      721 tctcgtcgtc gagccggccg gcgaccgtca ggcgctcggg caggcggacg accacgcgcg
      781 ggtgtcgggc gcggacgtgc tccgcgaggc ggcgcacgaa gccgtcttcc gccaccggct
      841 tgaaggcctc gaactgttcc gaacgaatct ttagcatcga atgcgcgcgc gtgggccgac
      901 cttcgtctcg ccgcggcccc gcgccctttc agttacagac ctcaaccgcc gatgatgacc
      961 gtcatctcgc cgcggacgat cttgttgggc gggccgaccg cttcgagcac ggtgtcgccg
     1021 aggcggcagg ccgggaggtt gttgatgagg acggtcttcg agccgtcgat gacgacgccg
     1081 gggccgtgcg gcgggacggg cagcagcgtc atgcacgagt ggatgtcggc gccgccggcc
     1141 gccccggtga tggccgtccc catcgtcgtg gcggcggtcg ccttccccgt ctcctcggcg
     1201 gccttggcgg cgggcgcgcc cggcgtcggc gcggcggcca ccgtcgccgc ctccagcgtt
     1261 ttgatggccg cgtcggagac ctgcttggcc gcctgcaacc ccgcggcggc cgccgcatta
     1321 actccgcgcc acgcgggcag ggcgccgatg agcacgttcg ggcttcccgg ccccggcgtc
     1381 aggacgggcg ggagcgggtg aacgacgttg tcggtgattc tggcggccgg ccctgtcggc
     1441 atttgtcttc ctccaagtaa agcggtgcgc ctctagtgat agacactccg ccaagaaaat
     1501 taacatggat ggacaggata gataggataa gaagatttga aatctcaaat ctgaaatctc
     1561 aaatcatctg ttcttcatcc tgcacatcct gtccatccat gttaactcct cagcccggcg
     1621 cgagcggctg tgttcgctgt tgcagcgcgc cggacgataa ctcgaagcgg aacatctcgt
     1681 gcgggtcttc gccttcgagc aggtaggccc tgacggggcc gaagatcgtc tctagctccc
     1741 gcgcgtcgca cgtcggcaga tagacgcgca gcacgcgcgg gtcgtagtag cgaaagagca
     1801 aaggcttgcc ctcggggtcg tagaccgtca ggaacgagcg gaagtgctga cgcatctcgc
     1861 gaaagccggt gtgcgcgagg gcgaagacgc accagtgttt gccccagcct tcggagacga
     1921 gccagtcggc aaactccgtc gcgggttcga gccggacgag atagggcgcg acttcggcca
     1981 tgtcgggcgc cagctccccg cggtagaggc aggcgaactc cggctggagg ccgtacaact
     2041 tatcgagcag ccccgcaacc gacgccccgt cgatgacggc gaaggcgttt gcgccgtcct
     2101 ccgtaaagag cgccgcgtcc aacgcttccg tgatctcttc tgtttccatc tgttcgggta
     2161 gccgcacggg ctacgagtcc tgctgcggtc atatgtcgca gaacggcgcc ccgttctgcg
     2221 ccgcctgtcg catcacgagc gccgccgggc tgtagacgac gggcacgccc ggctgcctcg
     2281 ccggcggcat ctcatgggcc tgccccggct cgcccgtgtc ggcttcgagc ggcgccgtcg
     2341 gggcggtggg cgaggcgccg gagccggagc cgccggagcc gccgctgttg atcatcacca
     2401 tcgtgccctt gatgaagatg ccgcccggat tgatgttgat gaagttgccg ccgaccttga
     2461 tggtcaacgc cgtcccggcc tcgatggtga cgttcatccc ggccttgagg tgtatctcct
     2521 gtccggcatc gacgttgaac ttcgtccccg ccttcgtctg cgcgtcctgg ccgaccttca
     2581 aggagtagcc gccgtcaacc tgctcgttct ggtcgcccgt caccttgagg tgcttgtcgc
     2641 cctcgacctg ttcgagctgg tcgcgcttga cgatgaggtg agtgtcgcgg ccgatccact
     2701 cgacgcggtc gttcttgacg cggccgtgca agtccttctc ggcgtggtgg aagaccttct
     2761 ccttgccttt ggtgtcgtcg aacgacatct cgttgaagcc gccgccgccc ttcgtcgagt
     2821 aggtcttaat cgtgctcttc atcttcgcgt cgggcagggc gtggggcggc atcgccccgc
     2881 cgttgtagag gcggccagtg atgatggggc ggtcggggtc gccttcgagg aaatcgacga
     2941 tcacctcctg accgatgcgc ggcacgaaca tcgcgcccca gcgcttgccc gcccagttct
     3001 gcgagacgcg cacccagcac gagctgttct cgtcgttctt gccctcgcga tcccagtgga
     3061 actgcacctt cacgcgcccg tacttgtcgg tgtaaatctc ctcgcccgcg gggccgacga
     3121 tgacggccgt ctgcgtgccc tgaatgacgg gcgtgggcgt cacgcgcggc ggccggtatg
     3181 gcgtggggtg cgggatgcac tcgaaggtgt tcgagtagcc gtcgatggcc tcagacgcgc
     3241 cttcggcgcc cgagccgtag ccggcggcga ggtcggccga gtgctggacg gaggtgagga
     3301 cgtaggggcc gttcatgtcg cggcggtagt ggcccttgag atcgacgcgg tagccggagg
     3361 cgagcgcggc gtagctgctc gcgccgcggg cgacgacgag cggtgagtcc tgctcctgca
     3421 cgcggacgcc gacgaggcgc tcgccctgat cgcgcgtcac gtagccgccg gggtagtcgt
     3481 aaatttcgta atgcctcgtg tcacggccct cgacgctggc cgtgagatcg agcgccggct
     3541 tctggaagtc gaagtcgttg gccgtgtact tgccggggcg cacctcctgc accttctgcc
     3601 actccttgac ggcctcgcgc gccgcgtctt cgccgatggc cgacgtgtag aaggccgcgg
     3661 gcagattggg gcagggcttg aactcgttgg gggcgtcggc gagaaccaaa gtgtgcttgt
     3721 cggcctcgtg ctcgaagaag tagaagatgc cctcatactc catgaggcgc gagacgaagt
     3781 cgaaggccgt ctcgcgatac tgcacgcagt actcgcgcac gtcgtagctg ccgtggaggc
     3841 ggttgctgaa gtcggtgaag ccgtaatcct gaaagacctt ctggatgatg tcgggcgcgg
     3901 agaggttctg gaagatgcgg cagtcgctcg tgcgcgtgag cagccagagc caggggacga
     3961 gcgtggcccg gtactcggtg aggtggcccg tcgtgccctc ctgcgcgtag ctggtcgtgt
     4021 cgccctgtga gactctgcta cagatgccgt tgatgtggcg cacggagccg tcggcgagac
     4081 tcaccttgac ggtcgcgttc ttgccaacca gcgcgtcgaa ggcgagggag cggttctcgg
     4141 agagcagacg gaggtcgaag cggaagagac tggagacgcc ttcgctcccc tcgaagcccc
     4201 ggaggaggag cacgtcctcg ccgagcggcg tgaagatttg gatccgtcga ttctcttgcg
     4261 tgaacttcgt tggcaagtct tacgtcctcc gctccccggc cacgccactg ggtcagtacc
     4321 gcgagcggta gcgagcgggt gtagttgcgt cagcgacccg ctcgctaccg ctcgcggtac
     4381 tgacttacct cacacccgca cactcactcg ctcaccaccc tacgtgatgt tatacacgaa
     4441 ctgcccgccg tcgccgaccc cgatgtggac gcgcgcgacc ggatgccccg ccgccatccg
     4501 cgacaggaat tcgcccgaca tctccggcag aagggtgcgc gtcaggatgt gatcgacgtt
     4561 gcgcgcgccc gactccacct ccttgcagcg gttggcgatc tcctcgatga cgcgctcgtc
     4621 gtagtcgaac tcggcgtcgt ggttttccga catgcgccgg ccgacttgcc cgagcttgag
     4681 cttgatgatc tcgcgcatcg cctcatcact gatcgggtaa tagggcacga cgaacatgcg
     4741 gccgaggagc gccggcttga agaccttaat caactccggc cggatggcct cgacgagcgg
     4801 ctccggctcg ggccgcgtgt cggggtcggc gcacaggttc atgatcgtgt cggtggcgac
     4861 gttcgaggtg agcaggatga ccgtgttctt gaagtcgatc tcgcggccct cgccgtcttc
     4921 gagcatgccc ttgtcgaaga cctgatagaa cagctccatc acgtccgggt gcgccttctc
     4981 gacttcatcg agcaggacga ccgagtaggg cttgcggcgc acggcctccg tcaacacgcc
     5041 gccctcgccg tagccgacgt agcccggagg cgatcccttg aggctggaga ccgtgtgcga
     5101 ttcctgatac tcgctcatgt tgatggtgac gaggttgcgc tcgccgccgt agagcgtttc
     5161 ggcgagcgtc agcgccgtct cggtcttgcc gacgccggag gggccgacga ggaggaagac
     5221 gccgacgggc cggcgcgggt cggtgaggtt ggcgcgcgag gtgcggatgc gttgcgcgat
     5281 ggagtcgagc gcgtgcgact gtccgatgac tctctgttcg aggatgtctt tgagacgcag
     5341 cacggtgttg atctcgtcgg ccatcatgcg gccgacgggg atgcccgtcc agccggagat
     5401 gacctcggcg atggcctgcg agttgacggt cgcctgcatg agcggcgtct cgccctgcac
     5461 ctgcgacagc tcgtcgttga gcttgccgag ttcggcgcgc aacccttccg tatcgacggg
     5521 cacggccggc gtcaacgcgg cggccgtggc ggcttccatg ttccccgcca tcgccgcggc
     5581 cgtcgccact tccggcttgc cgccgttgct gccgccgacg gcctgctcca actgcgtgcg
     5641 gacttcgcgg atgcgttcga cgatgccgcg ctccttgtcc cactgctccc tgagccgcgc
     5701 gaggcgttcc tcctgttcag tcagcgccgc ccttagctca tcgacgcgct tggcgtggtc
     5761 ggtgccggta atcgactcgc gctccagcga gcgcagctcc gtcccgatct gatcgatgcg
     5821 ccgctgcgcg tcctcgacgg gcggcggcgt cgccccctgc ccgatggcga ccttggcgca
     5881 ggccgtatcg agcacgctca cgcacttgtc ggggagctgg cggtcggaga tgtagcggtg
     5941 cgacagacgc accgagtctt cgatggcctc ttcgagaacg cgcaccttgt ggtggttctc
     6001 caagtagccg gcgaaggcgc gcatcatcac gatggccttg tcctcggtcg gctcctcgac
     6061 cttgacgacc tgaaagcggc gcgttaaggc cgggtctttc tcgaaatatt ttttatactc
     6121 ggcccacgtc gtcgcggcga tggtgcgtag ctcgccgcgg gcgagcgcgg gcttgaggag
     6181 gttggcggcg tcgttctgtc cggccgcgcc gcccgcgccg atcatcgtgt gcgcctcgtc
     6241 gatgaagagg atgatgggct gcggcgacga tttcacttcc tcgatgacgc ccttcaggcg
     6301 attc
//
LOCUS       DASVES010000209         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_583729, whole genome shotgun sequence.
ACCESSION   DASVES010000209 DASVES010000000
VERSION     DASVES010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583729"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /gene="hflX"
                     /locus_tag="VF240_08180"
     CDS             complement(<1..1604)
                     /gene="hflX"
                     /locus_tag="VF240_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7175245.1"
                     /translation="MFERRFPRLEDFISSVQGNTQGLKPNQLRRIEKLYSRRIAPQQI
                     VTPEFARQLTELSHETGRQLGALVDRKGYVEAVVVGDARRIEWPDLKRTRVGSDRFRG
                     LRALHTHLRGEDLTQDDLNDLALLRLDLVAVVDVDARTGLPGRVRAAHLLPTTAAEVA
                     DGKHDLAYGFLEAPFPSQLDVNFLELIDSLEEEMARNRSAGRRAEVRERAILVGVTTG
                     SLADAEESMAELRELAASAGLFVINDIIQRRQKIDPRTVLGKGKLEELIVRALRLGAD
                     LIIFDRELSPGQVRSINEATDLKVIDRAQLILDIFAQRAQTSEGKIQVELAQLKYLLP
                     RLIVGQDSAFSRLAGGIGGRGPGETKLETDRRRVRDRINRLDKEIDAQRMRRKERRKE
                     RTRRELPVISIVGYTNAGKSTLLNTLTASRVHAEQRMFATLDPTSRRLRLPREQEVII
                     NDTVGFIRDLPPDLLAAFRATLEEIDQSDLLVHLVDASNPRWQQQLESVENILGELGH
                     TQIPRIVAFNKADLLTPDDVDALLRQA"
     gene            2062..3090
                     /locus_tag="VF240_08185"
     CDS             2062..3090
                     /locus_tag="VF240_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175246.1"
                     /translation="MGDSLEIVRVDPSAAIAGGEVVVECRNFERRRIRDGKVFFKEKR
                     AWLVGASPRRLLALLPDFEEGGEVDVVLEDGENVRSAPARIVAGKKLAEDLHLVASPA
                     VDPGDGSVVVTRSGSRGQQVPVSLYRIDRDGELHGLHGEITNPTGIAFDDRGRMYVSS
                     RLDGTVYRVTADQDVTPFASDLGVATGLAFDRLGYLYVGDRQGKIFRVNGAGESEEWA
                     RSEASVSAYHMAFGPDASLYLTGPTVSSYDTVTRIDEHGRRSTFFRGLGRPQGLAFDR
                     DGTLYVAASYRGRRGVVAIDREGREARLVVAGMNVVGLAFNSAGDMLVATNEAVYSLP
                     LGIRGTLV"
     gene            3241..3951
                     /locus_tag="VF240_08190"
     CDS             3241..3951
                     /locus_tag="VF240_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7175247.1"
                     /translation="MEDPASIVANLLIYLVVLLMAISAHEAAHAWMSYKFGDDTAYLL
                     GRVTLNPVAHTDPIGTLLIPIVGFIGASAGVTIPLIGWGKPTPVNPLRWRNKDVANVM
                     VSLAGIMVNTLLAIVAFVILKALLASGTLDRSLFIYVVFDRGGTGLLIEPVAKFLNSM
                     LIMNASLAVFNLMPFPPLDGSKVLETILPASAQPILDTLEQYGYIILMLAVFLGLFGA
                     IFRPVALLIYSLLLGFPG"
     gene            3991..>4782
                     /gene="trpS"
                     /locus_tag="VF240_08195"
     CDS             3991..>4782
                     /gene="trpS"
                     /locus_tag="VF240_08195"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7175248.1"
                     /translation="MAKRIFSGAQPTNNLHLGNYIGALRNWVDLQHEYESFFCIVNLH
                     AVTLPQDPRRLAEKTRELARVYLAAGIDPKVSTVFIQSDVPEHTELAWVLNCVARISE
                     LERMTQFKDKARKAREQVGAGLLTYPTLMASDILLYQTDLVPVGHDQKQHLELTRDIA
                     SRFNRDYGETFRLPDVFIPKVGAKICALDDPSKKMSKSDENDAGRISLTDDADAIRRK
                     FKRAVTDSGAEIRFDESRPGVTNLLSVYQIMTGRAPAEIEAHFEGK"
BASE COUNT          783 a         1530 c         1607 g          862 t
ORIGIN      
        1 gcctgtcgca ggagcgcgtc cacgtcgtcg ggcgtgagca ggtcggcctt gttgaaggcg
       61 acgatgcggg gaatctgtgt gtgaccgagt tcgccgagga tgttttcgac cgattcaagc
      121 tgctgttgcc agcgcgggtt cgaggcgtcc acgagatgca cgaggaggtc gctctggtcg
      181 atctcttcga gcgtggcgcg gaaggcggcg aggagatcgg gcggtaggtc gcggatgaag
      241 ccgaccgtgt cgttgataat cacctcctgc tcgcgcggca gacgcaggcg gcgcgaggtc
      301 gggtcgagcg tggcgaacat gcgctgctcg gcgtggacgc ggctggcggt gagcgtgttg
      361 agcagggtgg atttgccggc gttggtgtag ccgacgatgg agatgacggg cagctcgcgc
      421 cgcgtgcgct ccttgcgacg ctccttgcgg cgcatgcgct gggcgtcgat ctctttgtcg
      481 agccggttga tgcggtcgcg cacgcgccgt cggtcggtct ccagtttcgt ctcgccgggg
      541 ccgcgcccgc cgatgccgcc ggcaagacgc gaaaacgccg agtcctgccc gacgatgagc
      601 cgcggcagga ggtatttcag ttgcgccagc tccacctgaa tcttgccctc actcgtttgc
      661 gcgcgctggg cgaagatgtc gaggatgagc tgggcgcggt cgatgacctt gaggtcggtg
      721 gcctcgttga tggagcgcac ttgtccgggg gacagctcgc ggtcgaagat gatgaggtcg
      781 gcgccgagac gcagggcgcg gacgatcaac tcttcgagct tgcccttgcc gagcacggtg
      841 cgcgggtcga ttttctgtcg ccgctggatg atgtcgttga tgacgaagag tccggcggag
      901 gcggccagct cgcgcagctc ggccattgac tcctccgcgt cggcgagcga ccccgtggtg
      961 acgccgacga ggatagcgcg ctcgcgcacc tcggcgcgtc ggccggcgct gcgattgcgc
     1021 gccatctcct cttcgagcga gtcgatgagt tcgaggaaat taacgtcgag ctgcgagggg
     1081 aagggagctt cgaggaagcc gtaggccaaa tcgtgcttgc cgtccgccac ctcggcggcc
     1141 gtggtcggca ggaggtgtgc ggcgcgcact cttcccggca accccgtgcg cgcgtccaca
     1201 tctacgacgg ccacgaggtc gagacgcaaa agagcgagat cattcaggtc gtcctgcgtg
     1261 aggtcttcgc cgcgcaggtg cgtgtgcagg gcgcgcaagc cccgaaagcg gtcggagccg
     1321 acgcgcgtgc gtttcaggtc gggccactcg atgcggcgcg cgtcgccgac gacgacggcc
     1381 tcgacgtagc ccttgcggtc aacgagtgcg ccgagctgcc ggcccgtctc gtgcgacagc
     1441 tccgtgagct ggcgcgcgaa ctccggcgtg acgatctgtt ggggcgcgat gcggcgcgag
     1501 tagagcttct cgatgcgccg cagttggttg ggcttgaggc cctgcgtgtt gccttgtacg
     1561 ctgctgataa aatcctccag acgcggaaag cgccgctcga acaccttcgc ccgcgccgcg
     1621 gcgacaaacc gctacgcagg cacacttcgc cccttcagcg tttccggccg cgtgtggcta
     1681 gagggagagg ggagcgagcg gtgtgagtcg gacgcttggg tgaaagctcg gacgtttctc
     1741 ttcgcagaga cacagttgcg gttggtcgtc acgcgtcgcg gaaactgccc ggtttgcttg
     1801 ccggcatggc ccgcgaaggt tcctgaccgt gccggattat agcacagcgc gtccgggccg
     1861 cacgcccgcg cggacggcct gcttggacag ttgcgacgcg tagtcgtatg atggcgtgca
     1921 acggcacgag ggcacaaaaa cgaacttgcg acggacagca tttgtgattt gaaattccag
     1981 atttcaaatc aacggcgata gcttgcacga gagccggccc gacggggctt caagggccgg
     2041 cggggcaggg gatgaaaagg gatgggtgat agtttggaga tcgtgcgggt cgatccttcg
     2101 gccgccatcg cgggcgggga ggtggtcgtc gagtgccgca acttcgagcg gcggcgcata
     2161 cgtgacggca aggtcttctt caaggagaag cgcgcctggc tcgtcggggc gtcgccgcgc
     2221 cggctgctcg cgctcctgcc cgatttcgag gagggcggcg aggttgatgt cgtcttggaa
     2281 gacggcgaga acgtgcgcag cgccccggcg cgcatcgtcg ccgggaagaa gctggccgaa
     2341 gacctgcatc tcgtcgccag cccggccgtc gatccggggg acggctcggt cgtcgtcacg
     2401 cgctccggct cgcgcggcca gcaggttccc gtctcgctct atcgcatcga ccgcgacggc
     2461 gaactccacg gcctccacgg cgagattacg aaccctacgg gaatagcctt cgacgaccgc
     2521 gggaggatgt acgtctccag ccggctcgac gggacggtct accgggtgac ggccgatcag
     2581 gatgtgacgc ccttcgcgag cgacctcggc gtggcgacgg ggctggcctt cgaccgcctc
     2641 ggctacctct acgtcggcga ccgtcagggg aaaatcttcc gcgtcaacgg cgcgggcgag
     2701 agtgaggagt gggcgcgctc cgaagcctcg gtctcggcct accacatggc ctttggccca
     2761 gacgcgtcgc tctacctgac ggggccgacc gtgtcgagct acgacaccgt cacgcgcatc
     2821 gacgagcacg gccgccgcag caccttcttc cggggcttgg gccgtccgca gggcctcgcc
     2881 ttcgaccgcg acggcacgct ctacgtcgcc gcctcctacc gcggccggcg cggcgtcgtg
     2941 gcgattgacc gcgaaggccg cgaggcgcgc ctcgtcgtcg ccggcatgaa cgtcgtcggc
     3001 ctcgccttca actccgccgg cgacatgctc gtcgccacca acgaggcggt ctacagtctg
     3061 ccgctcggga ttcgggggac gctggtgtag tgagcagtaa gcagtgagca gtaagcagta
     3121 ggccgcccgt gcgggccacc gttcaacatc gtacttgttt ttaactgctc actgcttact
     3181 gctcactgct cactcttttg acaccctcga aatgggcgtg atacgtttcc tgcatagagt
     3241 atggaagacc ctgcaagcat cgtcgctaac ctgctcatct acctcgtcgt gttgctgatg
     3301 gcgatctcgg cgcacgaggc ggcgcacgcg tggatgtctt acaagttcgg cgacgacacg
     3361 gcctacctgc tcgggcgcgt gacgctcaac cccgtggcgc acaccgaccc catcggcacc
     3421 ctgctcatcc ccatcgtcgg cttcatcggc gcgagcgccg gcgtcaccat cccgctcatc
     3481 gggtggggca agccgacgcc cgtcaatccg ctcaggtggc gcaacaagga cgtggcgaac
     3541 gtgatggtct cgctggccgg catcatggtc aacacgcttc tggccatcgt tgccttcgtc
     3601 atcctcaagg cgctgctcgc ctcggggacg ctcgaccgtt cgctgttcat atacgtggtg
     3661 ttcgaccggg gcgggacggg cctgttgatt gagccggtgg caaagttcct gaactcgatg
     3721 ctgattatga acgcgtcgct ggctgtcttc aacctgatgc cgttcccgcc gctcgatggg
     3781 agcaaggtgc tggaaaccat cctgccggcc agcgcgcagc ccatcctcga tacgctcgaa
     3841 cagtacggct acatcatcct gatgctggcc gtcttcctcg gactgttcgg cgcgatcttc
     3901 cgccccgtgg ccctgttaat ctacagtttg ctgttaggat ttcctggttg aggggccgcc
     3961 ggccgcgccg gacgaggctt accgcgagag atggctaaaa ggattttcag cggcgcacag
     4021 cccaccaaca acctgcatct cggcaactac atcggcgcgc tgcgcaactg ggtcgatttg
     4081 cagcacgagt acgagagctt cttctgcatc gtcaatctcc acgccgtcac gttgccgcaa
     4141 gacccgcgac gcctcgccga gaagacgcgg gagctggcgc gcgtctatct cgccgccggc
     4201 atcgacccga aggtctccac cgtcttcatc cagtcggacg tgcccgagca cacggagctg
     4261 gcgtgggttt tgaactgcgt ggcccgcatc tccgagcttg agcggatgac gcagttcaag
     4321 gacaaggcgc gcaaggcccg cgagcaggtt ggggcgggac tcttgaccta cccgacgctg
     4381 atggcctccg acatcctgct ctaccagacc gacctcgtcc ccgtcggcca cgaccagaag
     4441 caacaccttg agttgacgcg cgacatagcc tcccgattca atcgcgacta cggcgagaca
     4501 tttcgcctgc ccgatgtctt catcccgaag gtcggcgcga aaatctgtgc gctcgacgac
     4561 ccgtcgaaga agatgtcgaa gtcggacgag aacgacgccg ggcgcatctc gctcaccgac
     4621 gacgccgacg ccatccgccg caagttcaag cgcgccgtca ccgactccgg cgccgagatt
     4681 cgcttcgacg agtcgcgccc cggcgtcacg aacctcctca gcgtctacca gattatgacg
     4741 gggcgcgcgc ccgccgaaat cgaagcccac ttcgagggca ag
//
LOCUS       DASVES010000210        16480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1082646, whole genome shotgun sequence.
ACCESSION   DASVES010000210 DASVES010000000
VERSION     DASVES010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082646"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..2302)
                     /locus_tag="VF240_08200"
     CDS             complement(224..2302)
                     /locus_tag="VF240_08200"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX7175249.1"
                     /translation="MSLTEMNFAQNFVENMGWALLHSVWQGALVALLLACALSALRRA
                     SASARYAAGAAALAAMLAAPALTFVHLDARSAAGGASARSSEATWAALEAGQSASDGD
                     DGYRRRLHEAGGATAGSTSASAPARWWTARGVAPAVRWLVVFWLLGVLVHTVKLYGGW
                     RVAGRLKRQGARPLGAVWEESLARLRLQLGVRRAVRLCESALVEVPTLVGWLKPVILV
                     PASALSGLSTQQLEALIAHELAHVRRHDYLVNLLQSAAETLLFYHPAAWWVSRQVRAE
                     REHACDDMAVAATVGGALSYARALATLEHLRAGVGSSSPVSLAVGADGASLVARVRRL
                     IGAPPIAPAPRRLTPLAASGLALLALACVYAGAQHAASGAAILDEQMIEGGDAAASGR
                     TVAVTFVAVPSFRPQLNSIETTRKATKKLLADMDAHQIRAVGFVGEGQLGSEDNRVRE
                     ARVDLLRAWLDAGHELGNQTYRHMNLYDTPLDAWQENVVRGESLLRRLLGERDQKLRW
                     FSYPYLNTGPDAETKRAAAEFLKGRGYSVHPVTIDSLDFLFANAHQEALRRNDAETAR
                     RVADEYVPYMERMMEFYEGLSRDVFGREIPQVLMLTTSPVVADQFDELASMLKRRGYR
                     FVTLDEAIRDEAYTQPETYTGEWGVSWLQRWGYTKNGALRREPYLPPYMRQFSKGEGL
                     PPEERKQTRD"
     gene            complement(2302..2688)
                     /locus_tag="VF240_08205"
     CDS             complement(2302..2688)
                     /locus_tag="VF240_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX7175250.1"
                     /translation="MPKRETPRPTDTELQILRVLWRRGPSTVREVHEALAATHPTVYT
                     TVLKLMQIMAEKGLVSRNEEQRAHVYEARLAREETQAQLLGDLLDRAFEGSATSLVMQ
                     VLSSKRASAEELEQIRAVLDELEGGE"
     gene            complement(2902..3822)
                     /locus_tag="VF240_08210"
     CDS             complement(2902..3822)
                     /locus_tag="VF240_08210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7175251.1"
                     /translation="MHDVIIIGAGPAGLSCAMWCDELGLDTLVLEQEEEVGGQLLWVY
                     NPVKNHLGVEAEDGRELRELFAAQVSDRDFDLWTQVRIEEVDLRAKRVRLGSGEELRS
                     IALVVATGVRRRRLGVPGEEELRGRGVIESGQRDRDVVAGRDVCVVGGGDAAAENALL
                     LAEVCPTVTLVHRGEKLSARPGFVERLRGEHCITVFTGANLLRVIGFEQVEAVEIMRR
                     GAIKPFQMAVGGVLVRIGVDPNTELFRSQLDVDARGYVRVDARQETSVENVFAIGDVS
                     NPLAPTVSGAVGAGATAAKVIASRLSRAAG"
     gene            complement(3915..4511)
                     /locus_tag="VF240_08215"
     CDS             complement(3915..4511)
                     /locus_tag="VF240_08215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7175252.1"
                     /translation="MATVKILGVSGSMREHSRSRRALDLVLEAARERGAETQLLDLHA
                     LDLPMYRPDGAKDAASVAGAAEAVNWAGAFVLASPDYHGSMSGAMKNFLDYFWTEFAG
                     KVFGYLCASHEKGLTVMDQMRTAVRQCYGWSLPYGVAIHGDEDFDAEGNVSRPAVERR
                     LRMLARDTVVYGSLIREQFARDLVALDEPHTFAARYRK"
     gene            complement(4571..5101)
                     /locus_tag="VF240_08220"
     CDS             complement(4571..5101)
                     /locus_tag="VF240_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175253.1"
                     /translation="MTKIMLRVLLPATLLIVFAATQEAAPAHGATASQKSGGAAQAAS
                     VVRTFYNDHFARRQCCFDEAGLKRRRQWFEPSLYNALLREVRKPAPADEAPYFNGDPF
                     TDSQEGPADFSVGHASAGGGRATVSVLLRWADKGKTVERRTLRIELTRTRGVWRIDDI
                     RYPDGQSLRGQLKQSG"
     gene            complement(5134..5766)
                     /locus_tag="VF240_08225"
     CDS             complement(5134..5766)
                     /locus_tag="VF240_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7175254.1"
                     /translation="MSDADRWDERYLRGEHASAEASRLLVRAVDDFALEEDSPAAAAS
                     SLKTTPQRALDVACGAGRHALVLAARGFRVTAVDASRVGIEMTLDRARARGLDLDARV
                     ADLARGEFTIEPDAYQLVCDFYYLQRDLFRAMRAGLRRGGLFVAAIHTVDEHPSARPA
                     NPDFLLRPEELREQFRGWEILHYHETEGRDEDAGEHRRPSAEIIARRPAA"
     gene            complement(5795..7129)
                     /locus_tag="VF240_08230"
     CDS             complement(5795..7129)
                     /locus_tag="VF240_08230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7175255.1"
                     /translation="MQTFETLAARAAARPTTQSRAPRAASTAALLILFIAACLFSAAC
                     DSGVGGGASGSGALRYNADDMALLAETVLPPQQRALLATDAEQRKEFVDDVAELLAVS
                     DEARKNGVADRPEMKQRLELTRAVALAQSYVRQQREAGVSPEQIVAQADVDAFLKEPG
                     TAERFEQDLQTVQSLGSMPPGDLPQEQKDRMKGEWGRLMLAARKAEESGFARERRVAL
                     SVEVQQSGMLAETFLKEKAESFTPTEQEIDAFLAKRQMSGEDARKTAEDVLRRARAGE
                     DFAGLAREYSTEPGAKKSGGSLGWFGRGRMVKAFEDAVFALQADQISDVVETDFGYHV
                     IKNEGRRTQEPTTPGAQPEEQVRARHVLITSTDKQKGGPTGGITREQAKTAVQEEKRQ
                     AFVDDLVKRAGVRLAEDFKVTAPTPPPNQMPSLGAPPASGGAVPVPPANPQR"
     gene            complement(7207..7524)
                     /locus_tag="VF240_08235"
     CDS             complement(7207..7524)
                     /locus_tag="VF240_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175256.1"
                     /translation="MFESDLLDPYERLLRIEVLGRAVEVPESNTLLRCFQFLSVNTIS
                     YGDFCWNGDCTNCQFWYHDAGQTEEQDRTALACRHKVREGLVVTRLSRFIEIEGINKG
                     INK"
     gene            7692..7904
                     /locus_tag="VF240_08240"
     CDS             7692..7904
                     /locus_tag="VF240_08240"
                     /inference="COORDINATES: protein motif:HMM:NF041212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HEX7175257.1"
                     /translation="MIYVKPGCPYCQQARDHYLAQGVKWTEYDAQTDRARRKEMLAYS
                     DGDPTVPCIVENGEYVGSGWGNPPRG"
     gene            8157..11795
                     /locus_tag="VF240_08245"
     CDS             8157..11795
                     /locus_tag="VF240_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175258.1"
                     /translation="MIDTTLLNYRITEKLGAGGQGTVYMAIDERLGRTVVIKVLAPEL
                     TAREVNLRRFEREARLASSLDHPNICTIYGLHEVDNLHFIAMQYIQGKNVRELVAGRP
                     LALPSALSVATQVADALTAAHARGIIHRDIKAGNVMITDAGLAKVLDFGLAKLLDETG
                     EERGDKPENIHLTEMGVPYGTATYAAPEQATGQKVDHRADIFSTGVLLYEMLAGTWPF
                     RGKTSVEVRYAVLHDTPQPISEARRDNPPPRLQAIIDRALAKDPNKRYQKIEQMRDEL
                     RAVVREVAGVGAGGSAAAGAEVSDIPGVVSTVRPRHEQQGGFGRSFRRWLRSLTGGEG
                     QPTSLPPAAARRGAAPAAGSTAPPRGELHDTPHLSVVDGQKSIAILPFRNLSNDPEVG
                     FYEFSLADAVITELARLKSLVVRPSSQIVKFQGKEIDPLEAGRELRVSTVLASSFLRA
                     GDKLRVNVQLLDVASGELLWSDRVDASAEDIIALQDTIAERIVEGLKLEHTSVEREGT
                     AAPATRNSAAYQEYLRARDHFARFLFHTLSADDCDAAIEHFQRAIALDQSFALAYSGL
                     GACYANRVLKGLGNSEDYELAEEAFTQALALDPDVVEARMLMVFVYLARGEKQKAREE
                     VARLRQRAPNEAAVYFVKGALHRLDGEYDRALRAYDRLARLDPAARVVASYNRARVLL
                     YQGRTDEAMTELDAGARKEPDHPLIKTFRAVALQRRGEHETAAAMLREVIATHPQMDG
                     VRPLLAIALIRAGDPDGARAELTDRVREAADADHDIAYWLGAACALLGQPDEAFRWLE
                     RAVTLGNGNRPWFESDPKLESLRSDPRFAELLRRIDEQAAPPDADGSTTELPSTSAAP
                     IAPAPAAPTDKTETGAPRATATASAAAYEEYLRGRDAGGRFIYHTLAREDSDAAVEHF
                     QRAIELDPNFALAHCALGSTYANLVIKGFGSSEDYERAEVSFQRALALDPQLLEARLH
                     MVFIHLARGEREKARAWVEILSEEAPNNVGVQFVKATLHRLSGEYEEALQCYDRMLRL
                     NPAERVVVSYNRARVLMYQGRHDDAFVELDLGASLGPDHPLIKTFRAVALQRRGDPSG
                     AADIMRDVLARHPRMDAVRPIYAICLARAGDAAAARAELTDDVRAAADADHDTAYWLA
                     SAHALLGEHDEAFHWLGRAIELGNENKPWFESDQNWNELRGDVRFGALMRRIDQSKQK
                     ATAVNRQP"
     gene            11907..12716
                     /locus_tag="VF240_08250"
     CDS             11907..12716
                     /locus_tag="VF240_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7175259.1"
                     /translation="MSTFVIGDVHGRRGQLQKLVGMLPFNPARGDTLVLLGDLVDRGE
                     DVPGTVADVIALRRSLPFESMYVLRGNHEQMLLDFVDEGARLWLHPAVGGDCTFEQYT
                     GRDLTDETRGDIDAARAELLRALPEDHLAFFRRRPLFHEDAFAIYVHAGLDTAKHPRE
                     SDSHQLLWARNQEFFKHYHGKPCVFGHTPTQLLPRMGRIGRHGIYICHSAIGIDTGFD
                     DRSPLSCLQLPEFNLYQTFADGHTDFHHISHFLPEPLRAFRVLCNQEGNTD"
     gene            12830..13288
                     /gene="bcp"
                     /locus_tag="VF240_08255"
     CDS             12830..13288
                     /gene="bcp"
                     /locus_tag="VF240_08255"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX7175260.1"
                     /translation="MITEGGQAPDFATRDTDGNEVSLSDFRGQKVVLYFYPKDDTPGC
                     TKQACSIRDSFSEFEERGIKVLGVSLDDQASHRKFADKYELPFTLLADTDHSVADAYG
                     VYGEKEFMGKRYMGVDRKTFLIDEQGRIRKVFNKVNVEEHADEVLEAFDD"
     gene            complement(13570..14520)
                     /locus_tag="VF240_08260"
     CDS             complement(13570..14520)
                     /locus_tag="VF240_08260"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX7175261.1"
                     /translation="MTRASSDTRDEAASAEVRAEIEEARAFLAEARSLVESRLDELVP
                     AASVEPASIHAAVRWSLFAGGKRLRPALVIASGRTFGAPDEKLLDTACAFELVHTYSL
                     VHDDLPSMDDDSLRRGRPTCHVRFGEATAILAGDALQTLAFQTIAEDETLDATARVAL
                     IGELARASGTPAGMVAGQAHDLAAETRADVGAAELELIHRRKTGALIASAARSGALIG
                     GAAPEELDAVTRYAHDLGLLFQITDDLLDVTATAHDLGKTPGKDARSQKATYPALHGL
                     EAARARAAEVYADACAALDTIEQPTGLLRSVARFILERRA"
     gene            complement(14501..14821)
                     /gene="xseB"
                     /locus_tag="VF240_08265"
     CDS             complement(14501..14821)
                     /gene="xseB"
                     /locus_tag="VF240_08265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7175262.1"
                     /translation="MAKRETTTRAGKGAAGQNFEGQLASLERIVRELERGDLPLERSL
                     ELFEQGVRLSRECQERLNEAERRIEVLLRDDAGRPVIAGEFETGVGSRAVEEEEEADD
                     ESVF"
     gene            complement(14874..15620)
                     /locus_tag="VF240_08270"
     CDS             complement(14874..15620)
                     /locus_tag="VF240_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175263.1"
                     /translation="MSAPTKGRGGRSRIVIDVDRARQESQARRRSGAGRGLRALSVVA
                     LVALGVLAVVVLGVFLWWGSYKKSPEYSLALLVDAAQRDDFRTVEGLIDGDAVAQALA
                     PQVAEKLAGAVPAGGDMAATRTRIAAAMPQLLPRVREGVRDEVARGVKAAAEGVGGRL
                     PFFALALGMKRYADVTEEGDSAAVTLNAVEGRPLVLSMRRDGERWKVAGVTDDALAAS
                     MAARIAPAISAPPAATAPTPRPAGRRRPRN"
     gene            complement(15620..>16480)
                     /locus_tag="VF240_08275"
     CDS             complement(15620..>16480)
                     /locus_tag="VF240_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7175264.1"
                     /translation="VRVQGEGAAREVAQAIALLNEFHRRACAEGRAHEGLDAIIVGRG
                     GGSTEDLWAFNEEEVARAIRASLVPVITGVGHETDFTIADFAADVRAATPTAAAELVA
                     AREDEVAARVEGLTRDMAVAFRLRLGDARVRVQDAAMSYGFDDVRSRLRSATARAGDA
                     SSVLERISTQRLVRERRRLDALTCRLSPASVSRRASRTRLRLAALRTGTETTVRARLE
                     EARSRLAVAAAALDALSPLAVLQRGYALAEGEGGRLLRAAPSVAVGERVRVRLAEGAL
                     RCRVEEVEGV"
BASE COUNT         2641 a         5889 c         5388 g         2562 t
ORIGIN      
        1 cacgtcgatc agcagcaggt cgtcgccgcg ccccttgcgc tccgcaaact ccgccggagt
       61 tatcgtcgag tagtccaaac gcctgtcacc tccgcgccca aagctaccaa cccgttcgtg
      121 agaacgcaaa ccgtgtgcgc ctgaaagagg aagggcgacg cttcacggaa gcgtcgccca
      181 cacggcgaag tttttgtccg gtgcggcctc ttctgcggcc tcttcagtcg cgtgtctgct
      241 tgcgctcctc gggcggaagg ccctcgccct tgctgaactg ccgcatgtag ggcggcaggt
      301 aaggctcccg tcggagcgcg ccgttcttcg tgtagcccca acgctggagc cacgagacgc
      361 cccactcgcc cgtgtacgtc tccggctgcg tgtaggcttc gtcccggatg gcctcgtcga
      421 gcgtgacgaa gcggtagccg cgccgcttga gcatcgacgc cagctcgtcg aattggtcgg
      481 cgacgacggg actcgtcgtc agcatcaaca cctgcggtat ctcgcgcccg aacacgtcgc
      541 gcgaaagccc ctcgtagaac tccatcatcc gctccatgta ggggacatac tcgtcggcca
      601 cgcggcgggc cgtctccgcg tcgttgcgcc gcagcgcctc ctgatgggcg ttggcgaaga
      661 ggaagtcgag gctgtcgatg gtcacggggt ggacgctgta gccgcgcccc ttgaggaact
      721 ccgcggcggc gcgtttggtc tcggcgtccg gccccgtgtt gaggtaaggg tagctgaacc
      781 agcgcaactt ttggtcacgc tcgccgagca gtcggcgcag cagcgactcg ccgcgaacga
      841 cgttctcctg ccacgcgtcg agcggcgtgt cgtagagatt catgtggcgg taggtctggt
      901 tgccgagttc gtgcccggcg tcgagccacg cgcgcaacag atcgacgcgc gcctcgcgga
      961 cgcgattgtc ttcgctaccg agttgcccct cgccgacgaa gccgaccgcg cgaatctggt
     1021 gtgcgtccat gtcagccaaa agcttcttcg tcgccttccg cgtcgtctcg atggagttca
     1081 actgtgggcg gaagctcggg acggcgacga acgtgacggc gaccgtgcgg cccgaggcag
     1141 ccgcgtcgcc gccttcaatc atctgttcgt caaggatggc ggcgccgctc gcggcgtgtt
     1201 gcgcgccggc gtagacgcag gcgagggcga ggagcgcgag gcccgaggcg gcgagcggcg
     1261 tgagccgtcg cggcgcggga gcgatgggcg gcgcgccgat gagacggcgg acacgcgcga
     1321 cgagcgatgc gccgtccgcg ccgacggcga gactgaccgg ggacgacgag ccgacgcccg
     1381 cgcgcagatg ttcgagcgtg gcgagggcgc gggcgtaaga gagagcgccg cccacggtag
     1441 ccgccacggc catgtcgtcg cacgcgtgct cgcgctcggc cctcacctgc cgcgagaccc
     1501 accacgccgc cgggtggtag aacagcaaag tctcggccgc cgattggagc aggttgacga
     1561 gatagtcgtg acgacggacg tgcgccagct cgtgcgcgat gagtgcttcg agctgttgcg
     1621 tgctcagacc cgagagcgcg ctcgcgggga cgaggatcac gggcttgagc cagccgacga
     1681 gcgtcggcac ctcgacgagc gccgactcgc acaggcggac ggcgcggcgc acccccaact
     1741 ggaggcggag gcgggcgagc gactcctccc agaccgcccc taacggcctg gcgccctgtc
     1801 gcttgagtcg cccggcgacc cgccagccgc cgtaaagttt tactgtgtgg acgagcacgc
     1861 cgaggagcca gaatacgacg agccagcgca cggcgggcgc gacgccgcgt gccgtccacc
     1921 agcgcgcggg cgcactcgcg gaggtcgagc cggccgtcgc gccgcctgcc tcgtgtaagc
     1981 gtctcctgta tccgtcgtcg ccgtcggacg cggactgccc agcttcaagc gcggcccacg
     2041 tcgcttcaga cgaacgcgcg gaagcgccgc ccgcggccga ccttgcgtcg aggtggacga
     2101 aggtcaacgc gggcgcggcg agcatcgcgg cgagcgcagc ggcgccggcc gcgtaacgcg
     2161 ccgaggccga ggcgcggcgc agtgcggaga gcgcgcacgc gaggagcagc gcgacgagcg
     2221 ccccctgcca gacggagtgg agcagcgccc accccatgtt ctcgacgaag ttctgtgcga
     2281 agttcatttc cgttaaagac atcactcgcc cccctccagt tcgtccagca ccgcgcgaat
     2341 ctgttcgagt tcttcggccg aggcgcgctt cgacgagagc acctgcatga cgagactcgt
     2401 ggccgagcct tcaaaggccc ggtcgagtaa gtcgccgagc agttgcgcct gcgtctcctc
     2461 gcgcgcgagc cgcgcctcgt agacgtgtgc gcgctgctcc tcgttgcgac tgaccagtcc
     2521 cttctcggcc atgatctgca tgagcttgag gacggtcgtg tagacggtcg ggtgcgtggc
     2581 ggccagcgcc tcgtgcacct cgcgcacggt cgaagggccg cgccgccaga gcacgcgcag
     2641 aatctgcaac tcggtgtcgg tcggtctcgg tgtctctcgc ttcggcatgg acaaagaatc
     2701 ctctcccgga cagggtgaac ggcaccatac tacgaagtct ttcgtattgt caacgaaaac
     2761 gttcgtagtt ccgccgaggc ggacgaatag aagttaataa tgatcgtttt tggaaaagct
     2821 cgtcacttga atgtggggag cagccattca ccgcggaggc gccgcaggaa gcggagttcg
     2881 cgccgcaggc ttgagcgacg ttcaaccggc cgcgcggctg aggcgcgagg cgatgacctt
     2941 cgcggccgtg gcgcccgcgc cgaccgctcc actcacggtc ggcgcgagcg ggttcgacac
     3001 gtcgccgatg gcgaagacgt tctccacact cgtctcctgc cgcgcatcga cgcggacgta
     3061 gccgcgcgcg tccacgtcga gctggctacg gaaaagctcc gtgttcggat cgacgccgat
     3121 gcggacgagc acgccgccga cggccatctg gaagggcttg atggcgccgc gccgcatgat
     3181 ttcgacggcc tccacctgct cgaagccgat gacgcgcaga agattcgcgc ccgtaaacac
     3241 cgtgatgcaa tgctcgccgc gcaggcgctc gacgaagccg ggccgggccg agagtttttc
     3301 gccgcggtgg acgagcgtca ccgtggggca gacctcggcg agcagcaggg cgttctcggc
     3361 cgccgcgtcg ccgccgccga cgacgcacac gtcgcgcccc gcgaccacgt cgcggtcgcg
     3421 ctgccccgac tcgatcaccc cgcgcccacg caactcctcc tcgcccggca cgccgagacg
     3481 cctgcgcctg acgcccgtcg caacgacaag ggcgattgaa cgcaactcct caccgctccc
     3541 gagcctgacg cgcttcgccc gcaggtcaac ctcttcaatc cgaacttgcg tccacaaatc
     3601 aaaatccctg tcgctcacct gcgcggcgaa caactcgcgc agctcgcgcc cgtcctccgc
     3661 ctccacgccc agatgattct taacaggatt gtagacccac agcagttgcc cgccgacctc
     3721 ctcctcctgc tccagcacga gcgtgtcgag gcccaactcg tcgcaccaca tcgcacacga
     3781 gagtccggcc gggccggcgc cgatgatgat cacgtcgtgc atgaaagtca ggagtcagga
     3841 gtcaggagtc agaagtcaga atgagaaggg cgaccctggt ttttattctg actcctgact
     3901 cctgactcct ggcttcattt ccggtagcgt gccgcgaagg tgtgcggctc gtcgagtgcg
     3961 acgaggtcgc gggcgaactg ttcgcggatg agggagccgt agacgacggt gtcgcgggcg
     4021 agcatgcgga ggcgtcgctc gacggcgggg cgggagacgt tgccctcggc gtcgaagtcc
     4081 tcgtcgccgt ggatggcgac gccgtagggg aggctccagc cgtagcactg gcgcacggcc
     4141 gtgcgcatct gatccatgac ggtgagaccc ttctcgtgcg aggcgcagag atagccgaag
     4201 accttgccgg cgaactcggt ccagaagtag tcgaggaaat tcttcatcgc gcccgacatc
     4261 gagccgtgat agtcgggcga ggcgaggacg aaggcgccgg cccagttgac ggcctccgcc
     4321 gcgccggcga ctgaggcggc gtctttcgcg ccgtcgggcc gatacatcgg aaggtcgagc
     4381 gcgtgcaggt cgagcaattg tgtctcggcc ccgcgctcgc gcgccgcttc gagcacgagg
     4441 tcgagcgcgc ggcggctgcg cgagtgctcg cgcatgctgc ccgacacgcc aagtattttt
     4501 actgtcgcca ccggatgagt ctccgtccgt ttcttttgta ctgctcgctc ttcgggcgcg
     4561 cacgcgcgcc tcagcccgac tgcttgagtt ggccgcgcag tgactggccg tcggggtagc
     4621 ggatgtcgtc gattctccag acgccgcgcg tccgcgtcag ctcgatgcgc agcgtccgcc
     4681 gctcgacggt cttgcccttg tccgcccagc ggagcagcac gctcaccgtg gcgcggccgc
     4741 cgcccgcgct cgcgtggccc acggagaaat cggccggccc ctcctgcgag tcggtgaacg
     4801 ggtcgccgtt gaagtagggc gcttcatcgg ccggcgccgg cttccgcact tccctcagca
     4861 acgcgttgta gagagagggc tcgaaccatt gccggcggcg cttcaggccc gcttcgtcga
     4921 agcaacactg gcggcgcgcg aagtggtcat tgtagaaagt gcggacgacc gacgcggcct
     4981 gcgccgcgcc gccgcttttt tgcgacgccg tcgcgccgtg ggccggggct gcttcctgcg
     5041 tcgccgcgaa gacgatgagg agcgtcgccg gcaacaggac gcgcaacatg attttcgtca
     5101 tcctcatatc tccggcttca cccgcccggc cgctcaggcc gcggggcggc gcgcgatgat
     5161 ctcggccgac ggccgcctgt gttcgcccgc gtcttcgtcg cgcccttcgg tctcatggta
     5221 gtgcagaatc tcccagccgc gaaactgttc gcgaagctct tcggggcgga gcagaaagtc
     5281 gggattagcg ggccgcgccg atgggtgttc gtccacggtg tgaatcgcgg cgacgaagag
     5341 gccgccacgc cgaaggccgg cgcgcatggc tcggaagagg tcgcgctgga ggtagtaaaa
     5401 gtcgcagacg agctggtagg cgtccggctc gattgtgaac tcgccgcgcg cgaggtcggc
     5461 gacgcgcgcg tccaaatcga gcccacgcgc gcgcgcgcgg tcaagcgtca tctcgatgcc
     5521 gacacgcgaa gcatcgacgg ccgtgacgcg gaagccgcgc gcggcaagga ccagtgcgtg
     5581 tcgccctgcg ccgcacgcca cgtcgagcgc gcgctgtggc gtcgtcttca aagacgaagc
     5641 ggcagcagcc ggcgagtcct cctccagcgc gaaatcatcg acggcgcgca cgagcagccg
     5701 cgaggcttca gcggaggcgt gctcgccgcg caggtaacgc tcgtcccacc tgtcggcgtc
     5761 gctcatcacg cgcgttcggc gccgcccgcg gccctcaccg ctgcgggttg gcgggaggga
     5821 ctggcactgc gccgcctgat gccgggggcg cgccgagcga cggcatctgg ttcgggggcg
     5881 gcgtcggggc cgtgaccttg aagtcttcgg cgaggcggac gcccgcgcgc ttgaccaaat
     5941 catcgacgaa cgcctgccgc ttctcttcct ggaccgcggt cttcgcctgc tcgcgcgtga
     6001 tcccgccggt ggggccgccc ttctgcttat cggttgacgt gatgaggacg tggcgcgcgc
     6061 gcacctgctc ttcaggctgg gcgcccggcg tcgtcggctc ctgcgtgcgc cggccttcgt
     6121 tcttgatgac gtggtagccg aagtcggtct cgaccacgtc gctgatctgg tcggcctgaa
     6181 gcgcgaagac ggcgtcctcg aaagccttga ccatccggcc gcggccgaac cacccgaggc
     6241 tgccgccgct cttcttcgcc cccggctcgg tggagtactc gcgcgcgagg ccggcgaagt
     6301 cttcgccggc gcgggcgcgt cggagcacgt cctcggccgt cttgcgcgcg tcctcgccgc
     6361 tcatctgccg cttggcgagg aaggcgtcga tctcctgttc ggtgggcgtg aagctctccg
     6421 ccttctcctt gaggaaggtc tcggccagca tccccgactg ttgcacttcg accgagagag
     6481 ccacgcggcg ttcgcgcgcg aatcccgact cctcggcctt gcgcgcggcc agcatgagcc
     6541 gcccccactc gcccttcatg cggtccttct gctcctgcgg gagatcgccc ggcggcatgc
     6601 tgccgagcga ctgcactgtc tgcaagtcct gctcgaacct ctcggccgtg cccggctcct
     6661 tgaggaacgc atcgacatcg gcctgcgcga cgatctgttc gggagagacg cccgcctccc
     6721 gctgctggcg cacgtagctc tgcgccagcg cgaccgcgcg cgtcagctcc agccgctgct
     6781 tcatctccgg ccggtcggcg acgccgttct tccgggcctc gtcggagacg gccagaagct
     6841 cggccacgtc gtcaacgaac tccttgcgct gctccgcatc ggtcgccagc agcgcgcgct
     6901 gctgcggcgg cagcaccgtc tcggcgagga gcgccatgtc gtcggcgttg taacgcagtg
     6961 cgccgctgcc cgaagcgccg ccgccgaccc cggagtcgca cgcggcgctg aagaggcagg
     7021 cggcgatgaa gaggatgagg agggcggcgg tggacgcggc gcgcggcgcg cgactctgtg
     7081 tagtgggccg cgcggcggcg cgtgcggcga gcgtttcgaa tgtttgcatg ttcggattaa
     7141 tccctcgcgc gtcggctcgg cgcgcgaacg tcccccggcg gggcgtcggc gcgcgcgccg
     7201 tgcgcgtcat ttgttgatgc ctttgttgat gccttcgatt tcgatgaagc ggctgaggcg
     7261 cgtgacgact aaaccttcgc gcaccttgtg gcggcaggcc agcgccgtgc gatcctgctc
     7321 ctcggtctgc cccgcgtcgt gataccagaa ctggcagttg gtgcagtcgc cgttccagca
     7381 gaagtcgccg taggagatgg tgttcacgga gagaaactgg aagcagcgca gcagtgtgtt
     7441 gctctcgggc acctcgacgg cgcggccgag cacctcgatg cggaggagcc gctcatatgg
     7501 gtctaacagg tcgctctcga acacaggata aaaaagttca aaggtcgaaa ctcggacgaa
     7561 cgggggcgaa agtggcgcgt cgagtatagc ggaaaactca ggccgcccgc gagcggcgtc
     7621 ggccttatcc attcgcgagc gcttttgcta aactgtcgga aacgagaagg aggtgcgaac
     7681 cattgggttt agtgatctac gtcaagccgg ggtgtcctta ctgccagcag gcgcgtgacc
     7741 actacctcgc gcaaggggtg aagtggacgg agtatgacgc gcagaccgac cgcgcgcggc
     7801 gcaaagaaat gctcgcctat tcggacggcg accccacggt gccctgcatc gtcgaaaacg
     7861 gggagtatgt cggctcgggc tggggcaacc cgccgcgcgg ttgaaccgtc cacgactaaa
     7921 ggccggccgt gcgccggccc gacaccgcgg gcgaagaccg aagcttttga atttactttg
     7981 aagaatgacg accggaggaa tacaatcccc ggtcgtcgcc atttcaagct gttagctgtt
     8041 agcgccgcac ggcagacgcc gtcgcctgaa tgccaccgtc gcgcatcgag ggccgctcgc
     8101 ttccggctga aagctaacag ctaacagcta atagctaaca gccaaccgct catcccatga
     8161 tcgacacgac cctcctcaac taccgcatca cggaaaagct cggcgcgggc ggacagggga
     8221 cggtctacat ggccatcgac gagcgtctgg gccgcaccgt cgtcatcaag gtgctcgcgc
     8281 ctgagctgac ggcgcgcgag gtcaacctcc gccgcttcga gcgcgaggcc cggctcgcct
     8341 cctcgctcga ccacccgaac atctgcacca tctatggcct gcacgaagtg gacaacctcc
     8401 acttcatcgc catgcaatac attcagggca agaacgtccg cgagcttgtg gcgggccgcc
     8461 cgctcgccct gccctcggcc ctctccgtcg ccacgcaggt cgccgacgcg ctcacggccg
     8521 cccacgctcg cggcatcatc caccgcgaca tcaaggccgg caacgtcatg atcaccgacg
     8581 cagggttagc caaagtcctc gacttcggac tcgccaagct cctcgacgag accggcgagg
     8641 agcgcggaga caaacccgag aacatccacc tcaccgagat gggcgtcccc tacggcacgg
     8701 ccacctacgc cgcgcccgag caggccacgg ggcagaaggt cgatcaccgc gccgacatct
     8761 tctccaccgg cgtcctgctc tacgaaatgc tcgccggcac gtggcccttc cgcggcaaga
     8821 ccagcgtcga ggtgcgctac gccgtcctcc acgacacgcc ccagcccatc agtgaagccc
     8881 gccgcgacaa cccgccgccg cgcctccagg ccatcatcga ccgcgcgctc gccaaagacc
     8941 cgaacaagcg ttatcagaaa atcgagcaga tgcgcgacga gctgcgcgcc gtggtgcgcg
     9001 aggtcgcggg cgtcggcgcc ggcggctcgg cggcggccgg cgcggaggtg agcgacatcc
     9061 ccggcgtcgt ctccaccgtg cggccccgac acgagcagca gggcggcttc ggccgcagtt
     9121 tccgacgctg gctgcgcagc ctgacgggcg gcgagggcca gcccacatcg ctgccgcccg
     9181 ccgcggcgcg ccggggcgcc gcgcccgccg ccggctcgac cgcgccgccg cgcggagagc
     9241 tgcacgacac tccgcacctg agcgtcgttg acggccagaa gtcaatcgcc atccttccct
     9301 tccgcaatct ctcgaacgac cccgaggtcg gcttctacga gttttctctc gccgacgccg
     9361 tcatcacgga gctggcccgc ctcaagtcgc tcgtcgtgcg gccctcctcg cagatcgtca
     9421 agtttcaggg caaggaaata gacccgctcg aagccgggcg cgagctgcga gtctcgaccg
     9481 tcctcgcctc gagcttcctg cgcgccggcg acaagctgag agtcaacgtg cagctcctcg
     9541 acgtggcctc cggcgaactc ctctggagcg accgcgtgga cgcctcggcc gaagacatca
     9601 tcgcgcttca ggacaccatc gccgagcgca tcgtcgaggg tctcaagctc gaacacacct
     9661 cggtcgagcg cgaggggacg gccgccccgg cgacacgcaa cagcgccgcc tatcaggaat
     9721 acctgcgcgc ccgcgaccac ttcgcgcgct tcctcttcca caccctctcg gccgacgact
     9781 gcgacgcggc aatcgagcac ttccaacgcg ccatcgcgct cgaccagagc ttcgccctcg
     9841 cctacagcgg cctcggcgcc tgctacgcca accgcgtcct caagggcctc ggcaattccg
     9901 aagactacga gctggccgaa gaggctttca cgcaggcgct cgccttagac cccgacgtgg
     9961 tcgaagcgcg catgttgatg gtcttcgtct acctcgcgcg cggcgagaag cagaaggccc
    10021 gcgaggaggt cgcgcgtctc cggcagaggg cgcccaacga ggcggccgtc tacttcgtca
    10081 agggcgcgct ccaccgtctc gacggcgagt acgaccgggc gctgcgcgcc tacgaccgcc
    10141 tcgcccgtct cgacccggcg gcgcgcgtcg tcgccagcta caaccgcgcg cgcgtcctcc
    10201 tctatcaggg ccgcaccgac gaggcgatga ccgaactcga cgccggcgcg cgcaaggagc
    10261 ctgaccaccc gctgattaag actttccgcg ccgtcgccct ccagcgccgc ggcgagcacg
    10321 agacggccgc cgcgatgctg cgcgaggtga tcgcgacgca cccgcagatg gacggcgtgc
    10381 gcccgctcct cgccatcgct cttatccgcg ccggcgatcc cgacggcgcg cgcgccgaac
    10441 tcaccgaccg tgtccgcgaa gcggccgacg ccgaccacga catcgcctac tggctcggcg
    10501 cggcctgcgc ccttcttggc cagccggacg aagcgttccg ctggctggag cgcgccgtca
    10561 cgctcggcaa cggcaaccgc ccgtggttcg agtctgaccc caagctcgaa tcgctgcgct
    10621 ccgacccgcg cttcgctgaa ctcctgcgcc gcatcgacga gcaggccgcc ccgcccgacg
    10681 ccgacggctc caccaccgaa ctcccttcga ccagcgccgc tcccatcgcc cctgctcccg
    10741 ccgccccgac agacaaaacc gagacgggcg cgccgcgcgc caccgccacc gccagcgccg
    10801 ccgcctacga ggagtacctg cggggccgcg acgccggcgg ccgcttcatc taccacacgc
    10861 tcgcgcgcga agactccgac gccgccgtcg aacacttcca acgcgccatc gaactcgacc
    10921 ccaacttcgc cctcgcccac tgcgccctcg gctcgaccta cgccaatctc gtcatcaagg
    10981 gcttcggcag ctccgaagac tatgagcgcg ccgaagtctc atttcagcgc gcgctcgccc
    11041 tcgacccgca actgctcgaa gcccggctcc acatggtctt catccacctc gcgcgcggcg
    11101 agcgggagaa ggcgcgcgcg tgggtcgaga ttctttcgga ggaggcgccc aataacgtcg
    11161 gcgtgcaatt cgtcaaggcc acgctccacc gcctctcggg cgagtacgaa gaggcgctcc
    11221 agtgctacga ccggatgctg cgtctcaacc cggccgagcg cgtcgtcgtc agctacaacc
    11281 gcgcgcgcgt cctcatgtat cagggccgac acgacgacgc ctttgtcgaa ctcgacctcg
    11341 gcgcctcgct cggccccgac cacccgttga ttaaaacttt ccgcgccgtc gccctccagc
    11401 gccgcggcga cccatcgggc gccgccgaca tcatgcgcga cgtgctcgcg cgtcacccgc
    11461 gcatggacgc cgtccggccc atctacgcca tctgcctcgc ccgcgccggc gacgccgccg
    11521 cggcgcgcgc cgaactcacg gacgacgtgc gcgccgcggc cgacgccgac cacgacacgg
    11581 cctactggct cgcctccgcg cacgccctcc tcggcgagca cgacgaggcg tttcactggc
    11641 tcggccgcgc catcgaactc ggcaacgaga acaagccgtg gtttgaatcc gatcagaact
    11701 ggaacgaact caggggggac gtgcgattcg gcgccttgat gcgccgcatc gaccaatcga
    11761 aacaaaaagc gacagccgta aatcgtcaac cgtaaatcgt caatcgtgtt ggagccggtc
    11821 tcacgttgag cgtaacagct caaattgaga ctgcgtcaac ttcgaacgat ttacgattca
    11881 cgatttacga ttcacggttc ttgcccatga gcaccttcgt catcggggac gtacacgggc
    11941 ggcggggaca gctccagaag ctcgtcggga tgctgccgtt caacccggcg cgcggcgaca
    12001 cgctcgtcct gctcggcgac ctcgtggatc gcggcgagga cgtgcccggc accgtcgcgg
    12061 acgtgatcgc cctgcgccgc tcgctgccct tcgagagcat gtacgtgctg cgcggcaacc
    12121 acgagcagat gctcctcgac ttcgttgacg agggcgcgcg tctgtggctc cacccggccg
    12181 tcggcggcga ctgcaccttc gagcagtaca ccggccgcga cctgaccgac gagacgcgcg
    12241 gggacatcga cgccgcccgc gccgagctgc tccgcgccct ccccgaagat catctcgcct
    12301 tcttccgccg ccgccccctc tttcacgaag acgccttcgc catctacgtc cacgccggcc
    12361 tcgacacggc caaacacccg cgcgagagcg actcgcacca actgctctgg gcgcgcaatc
    12421 aggagttttt caaacactat cacggcaagc cctgcgtctt cggccacacg ccgacgcagc
    12481 tcctgccacg catggggcgt atcggccgcc acggcatcta catctgccac agcgccatcg
    12541 gcatcgacac cggcttcgac gaccgctcgc ccctctcctg tctgcaactg cccgagttca
    12601 acctctacca gaccttcgcc gacggccaca ccgacttcca ccacatcagc catttcctcc
    12661 ccgagcccct gcgcgccttt cgcgtcttat gcaatcagga ggggaacacg gattaaacgg
    12721 atgaagacgg attagcacga atccgtcttc atccgtttaa tccgtgttcc ctgcccgcgt
    12781 ttgaatctac gcggaatctc tgtaagactg ccggtggagg tttccaccga tgattacaga
    12841 aggcggacag gcgccggact tcgccacgcg cgatacggac ggcaacgagg taagtctttc
    12901 ggacttccgc ggccagaagg tcgtgctcta tttctacccg aaggacgaca cgcccggctg
    12961 caccaagcag gcgtgctcga ttcgcgactc cttctcggag tttgaggagc gcggcatcaa
    13021 ggtgctcggc gtctcgctcg acgatcaggc gtcgcaccgg aagtttgcgg ataagtatga
    13081 gctgcccttc acgctgcttg cggacaccga ccattcggtg gccgacgcct atggcgtcta
    13141 cggcgagaag gagttcatgg gcaagaggta catgggcgtt gaccgcaaga ccttcctcat
    13201 cgacgagcag ggccgcatca ggaaggtctt caacaaggtc aacgtcgaag agcacgccga
    13261 cgaagtgctc gaagcgtttg acgattaaga gcgcgttaaa ttaaaccgcg agggtgacaa
    13321 agccgagtcg cgcgcgtcaa ccagccgcgg agcggcggca gaatgtagcc acgggcgtta
    13381 gcccgtggaa cagtcccgcg aggcgcagcg aagccccgga ggggcgaaag aactttcaac
    13441 cttgagcgca tggctcgttg aggttcttcc gcccctccgg ggcttcgact tttttatcga
    13501 cctgtcccca cggctcgcgc cgtgggctac attctgccgc cgcttacagc ggctccttga
    13561 atccgtcttt cacgcgcgcc tctccaaaat aaaacgcgcg acggagcgca acaagcctgt
    13621 gggttgctcg attgtgtcga gcgccgcgca cgcgtccgcg tagacttccg cggcgcgcgc
    13681 gcgcgctgct tcgagaccgt ggagggccgg gtaagtggcc ttctgcgagc gcgcgtcctt
    13741 gccgggggtc ttgccgaggt cgtgggcggt ggcggtcacg tcgagcaggt cgtcggtgat
    13801 ctggaagagg agaccgaggt cgtgcgcgta gcgcgtgacg gcgtccagct cttcgggcgc
    13861 ggccccgccg atgagagcgc ccgagcgcgc ggccgacgcg atgagcgcgc ccgtcttgcg
    13921 ccggtggatg agttccagct ccgccgcgcc cacgtcggcg cgcgtctcgg cggcgaggtc
    13981 gtgcgcctga ccggcgacca tccccgcggg cgtgcccgag gcgcgcgcaa gctcgccgat
    14041 gagcgcgacg cgcgccgtcg cgtcgagagt ttcgtcctcg gcgattgttt gaaaggcgag
    14101 cgtttgcagg gcgtcgccgg cgaggatggc ggtcgcctcg ccgaagcgca cgtggcaggt
    14161 cggccgtccc ctgcggagcg agtcgtcgtc catcgacggc agatcgtcgt ggacgagcga
    14221 gtaggtgtgg acaagctcga aggcgcaggc ggtgtcgagg agtttttcgt cgggcgcgcc
    14281 gaaggtgcgt cccgaggcga tgacgagtgc ggggcgcagg cgcttgccgc ccgcgaacag
    14341 gctccagcgg acggccgcgt ggatcgaagc gggttcgacc gacgcggcgg gcacgagttc
    14401 gtcgagacgc gactcgacga gagagcgcgc ctcggccaga aatgcgcgcg cctcttcaat
    14461 ctcggcgcgc acttcggcgg acgcggcttc gtcgcgggtg tcagaagacg ctctcgtcat
    14521 ccgcctcttc ttcctcttcg acggcgcggc tccccacgcc cgtctcgaac tcgccggcga
    14581 tgacggggcg gcccgcgtcg tcgcggagca agacctcgat gcgccgctcg gcctcgttga
    14641 gtcgctcctg acactcgcgc gaaagcctca cgccctgctc gaacagctca aggctgcgct
    14701 ccagcggcag atcgccacgc tcaagctcgc gcacgatgcg ctcaagcgag gcgagctgcc
    14761 cctcgaagtt ctgcccggcg gcgcccttac cggcgcgcgt ggtcgtttct ctcttcgcca
    14821 taattatctc ccgcggccga cgcgctgctg ccgtcgtgtc ggccgcggcc ggatcagttt
    14881 ctcggcctcc ttcttccagc ggggcgcggc gtcggggccg tcgccgccgg cggggcggag
    14941 atggccgggg cgatgcgcgc ggccatcgag gcggccagcg cgtcgtccgt gacccctgcg
    15001 accttccagc gctcgccgtc gcgccgcatc gacaacacga gcggccggcc ttccacggcg
    15061 ttcagcgtga cggccgccga gtcgccctct tccgtcacgt ccgcgtatcg cttcatcccg
    15121 agcgcgagcg cgaagaaggg caggcggccg ccgacacctt ccgccgcggc cttgacgccg
    15181 cgcgccacct cgtcgcgcac gccctcgcgc acgcgcggca aaagctgcgg catcgccgcc
    15241 gcgatccgcg tgcgcgtcgc cgccatgtcg ccgcccgccg gcacggcgcc cgccagcttc
    15301 tcagcaacct gcggcgcgag cgcctgcgcg acggcgtcgc cgtcgatcaa cccctcgacc
    15361 gtgcggaagt cgtcgcgctg cgcggcgtcc acgaggagcg cgagcgaata ttcaggactc
    15421 ttcttgtagc tgccccacca gaggaagacg cccagcacca ctacggcgag gacgcccagc
    15481 gcgacgagcg cgacgacgga cagggcgcga aggccgcggc ccgcgcccga gcgacgccgc
    15541 gcctgcgact cctgccgcgc gcggtcaacg tcgatcacga tgcggcttcg gccgccgcgc
    15601 cccttcgttg gtgccgacat caaactccct ccacttcctc aacccggcaa cgcagcgccc
    15661 cttcggcgag ccgcacgcgc acgcgctcgc ccaccgccac cgacggggcc gcgcgcagca
    15721 gacgccctcc ttcgccttcg gccagcgcgt agccgcgttg caggacggcg agcggcgaga
    15781 gcgcgtcgag cgccgcggcg gcaacggcga ggcgtgagcg cgcttcttcc agacgtgcgc
    15841 gcaccgtcgt ttccgtcccc gtgcggagcg ccgccagacg caggcgcgtg cgcgaggcgc
    15901 gcctcgacac gctcgcgggc gacagtctgc acgtgagcgc gtcgagccgc cggcgctccc
    15961 gcacgaggcg ctgcgtcgaa atccgctcca acacacttga agcatcgccc gcccgcgccg
    16021 tggccgagcg caggcgactc cgcacgtcgt cgaagccgta ggacatggcc gcgtcctgca
    16081 cgcgcacgcg cgcgtcgccg aggcgcaggc ggaaggcgac ggccatgtcg cgcgtcagtc
    16141 cctcgacgcg cgcggccact tcgtcttcgc gcgcggcgac gagttcggcg gcggccgtcg
    16201 gcgtggcggc gcgcacgtcg gccgcgaagt cggcgatggt gaagtcagtc tcgtgcccca
    16261 cgccggtgat gacggggacg agcgaggcgc ggatggcgcg cgccacctcc tcctcgttga
    16321 aggcccagag gtcttcggtg gagccgccgc cgcgcccgac gatgatggcg tcaagtccct
    16381 cgtgcgcgcg cccctcggcg cacgcccgcc ggtgaaactc gtttaagagg gcgatggcct
    16441 gcgccacctc gcgggccgct ccctcgccct gcacgcgcac
//
LOCUS       DASVES010000211        20296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1144421, whole genome shotgun sequence.
ACCESSION   DASVES010000211 DASVES010000000
VERSION     DASVES010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20296
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144421"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..966
                     /locus_tag="VF240_08280"
     CDS             124..966
                     /locus_tag="VF240_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX7175265.1"
                     /translation="MTLTEQHVKKYEEDGFLFFPEHLSAADIEPLREQLPAVYAEDSA
                     RCVLEEGGRVVRSVYASHTTNETFRRLSEDPRLVAKAMKILGGEVYIHQFKINAKAAF
                     GGDRWEWHQDYVFWREEDGMPTARVTNVAVFLDEVNEFNGPLLLIPGSHRQEVIEVEG
                     RSEIPAAYADSPAWITNLTAELKYSLPRDIVAGLVKRHGIVAPKGPAGSVLIFHPNLV
                     HGSVQNISPFERTIVCVTYNSIFNTPPALEKMRPAFLSSRDFTPIRLRDDEHRAAREE
                     KERR"
     gene            963..5585
                     /locus_tag="VF240_08285"
     CDS             963..5585
                     /locus_tag="VF240_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX7175266.1"
                     /translation="MKLALAMNVPYARVYGGANRSNRALAEALAARGHEVHVITPALA
                     VPSTITREDFVAGLAAEGVAVRSEGSVDRFNLGGVEVHALAEQSQLRGYLTQALGRLE
                     PDWVLVSSEDPSQNLLDAALQTCPSRVVYMALTPPMLPFGPVSLYPGEARTALLGRAA
                     GIVSISDYLARYIKEWAGFESFVFVPPHYGAGPFPDFGRFDDGYVLLMNACAMKGLPV
                     FLSLARMLPRVSFAALHGYGTTEADREALKSLPNVSILENSKNLDDIFRQTRVLLMPS
                     LWAEGFPLAVIDAMLRGIPVMAAAHSGLVEAKLGTDYLLPVRPIEHFADSLDDNMLPS
                     PIIPEQDVEPWRDALVELLTDGDVYRRQSEAARTAARAYVARLGVEPLEEFLRGLETR
                     PRPGGMRPETPEAKKDDAFASAAALTPEQKALLMLRLRKKARAAQVVETGKNSIPSVS
                     RDLNTPLSFAQNRLWFLDQLEPGNPFYNCPIAIRLRGRLDAAALAGALGEIVRRHEVL
                     RTTFKLVGDRPIQIISEREQELPVVDLSGRPGAEQEARRLLYEEARRPFRLDEGPLLR
                     TTLIRMSEHEHVLVAVVHHIVADGWSTGILVREIKTLYESFSRGESSPLAELQIQYAD
                     FAHWQHEHLQGERMEKLINFWEQQLGGNLPVLELPADRPRANVQTFAGARHALALPRS
                     LTEELGALCRAEGATLFMLLLSAFKVLLHRHTRQADIIVGAPIANRNRREIEDLIGFF
                     VNTLVLRTDISGDPTFQELLRRVSRVTLEAYDHQDLPFEKLVEVLQPGRDMGHSALFQ
                     VMFVLQNAQLAPLELPGLMLTPVDLHSGTSKFDLTLEMVEAEEGLTALLEYNTDLFDA
                     ATAEGMLEQYQTLLEAVVREPRSKVSALPLLSPEAERRLLSEFNDTAVAFPPPHCIHQ
                     IFEEQVELTPDAVALVFEEEQLTYRELNRRANQLAHHLRSFGVGPDVPVGICMGRSIE
                     TIIAVLGTLKAGGAYLPLDPVYPAERLAFMLEDCQASVLLTQSDLLGALPPGRARVLC
                     LDDAREELNGESDANPRSGVAEENLGYIIYTSGSTGRPKGVSLPQRALVNLLRWHCAT
                     LRPGARTLQFASLSFDASFHEIFAALFTGGTLYLMQEGLRRDMNALAVLMRDQAIEKI
                     ILPVVVLQELAAEARERGHEFPRLKEVMATGEQLQVNKSVIDWFDDMQGGPRGGGCSL
                     HNHYGPSETHVVTSYTLPPAPRNWPTYPPIGRPVANTQIYILDAQLNPTPTGVPGELY
                     IGGANLARGYFNRPDLTAEKFIPNPHGDEAGGRLYKTGDLARYRADGNIEYLCRIDHQ
                     LKVRGFRVEPGEVESVLGSHPAVEKTVVLLREFAPGDRRLVGYVVGRQGPRPSASELR
                     EFLLRKLPEYMIPSAYVFLDALPLTPNGKLDRDALPMPDSARPDVGGGFVAPRTEIEK
                     SLAEIWAGVLGIDLVGVNDNFFELGGHSLLMIQIVSRISEALHVELPLKDFFQLPTVA
                     EQAAAVGRLMAEGSEQRGIEQLLDDLDQLSPEEVRRLLDERALTQN"
     gene            5710..13020
                     /locus_tag="VF240_08290"
     CDS             5710..13020
                     /locus_tag="VF240_08290"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX7175267.1"
                     /translation="MLLEEKGRGPRDERIPARGEVSPSSPLSFSQQRLWFFDQVTPGS
                     PLHNLSSILRARGALNLNAFEQSLNELVRRHESLRTTFPADEGRPLQLVAAPRPLQIT
                     VNDLGDLPETAREAEALRVAAEEGRRPFDLARGPLMRASVLRLGPEDHLLVFTTHHIV
                     SDGWSQGVIVREMSELYRSFLTGAQVRLPELPITYADFANWQRETLRGEALEADLTYW
                     KRALDGVAPLDLPLDRPRPAVPDFRSEEEVFELPEHLSEALRALSRQEGVTLYMTLLA
                     AFEVLLRRYTGQTDISVGSSVANRTRREIENIVGCFVNILVLRTDLSGDPTIGELLGR
                     VREVCLGAYAHAELPFERVVGEVQTDRNLNHNTLFQVAFILMSAPAPDLQLPGLSLSR
                     VEINHGLTEYDLSLMLAEEGQRLVGRLTYRSELFGRDTARRMLGHWRRILEGFVADPH
                     RRLSELELLSNAERQHLLHDLNQTATDYEREMCVHEVFERQAELAPDAVAVVHDGGQL
                     TYGELNRRANQLARHLRSLGVGPEVRVGVCVERSPEMIVGLLAILKAGGAYVPLDPSY
                     PLERLTFMLEDSAAPVLLTQEHLLDVLPATWASVVCLDSDWELVSRQSGEDVRGGAAP
                     ENLCYVMYTSGTTGRPKGVSVTHRGVVRLVRETNYVNQTPHDTFLQFAPISFDASTFE
                     VWGSLLGGARLALAPASTRSPEELAETIRTHRVTILWLTAALFHQMAEHYPESLFGVR
                     QLLAGGDVLQPSQVRKMLRGPAENLLINGYGPTENTTFTCCHVMSAGDDLEGAVPIGR
                     PIANTQVYILDEHMRPLPIGLPGELYIGGDGLARDYLNRPGLTAEKFVPHPFADGPGE
                     RLFRSGDRARYLVDGNIEFLGRADGQVKIRGFRIELEEVESALLRHPSVGQCVVVARE
                     ERPGDKRLVAYVVSGLGQPAEPEQLRGFLKLRLPDYMLPSAFVALEALPLTPSGKVDR
                     RALPAPETQRAQPTQNFVPPGTPAESLLADVWARVLGVERVGAHDNFFDLGGDSIRSI
                     EVCALAKKAGVEVSVQQLFECQTIHELARVAFSPELVTPEPASVPFSLISDDDRLALP
                     EDVEDAYPLTRLQAGMIFHSGYAGDAATYHDIFSYHLSGPCVPELMRRATAELVSRHA
                     VLRTSFNLTRYGAAMQLVHREVELPFEVADLRHLSEAEQETMIGESMETERARRFDWE
                     QAPLLRVQLLRRGEHTYQLTLSFHHAILDGWSLASLLTELYNRYVSLLEGADAAAAPP
                     PETAFRDYVALELDALESAEHRSYWAGKLNDSTVIKLPRRPASMLAGRDRRVRELLVP
                     LTAEECGALRNLARAEGVALKSVLLAAHFRVMSLLAAQPDVLTGVVSNGRPETADGER
                     VLGLFLNTLPLRLKLSRGTWRDLIRQTFEAEGEALPFRRYPLAEMQQVHGSGQPLFET
                     CFNFTHYHVFEGIQESGKVRVLGGASAAETNFTLLTNFNVDPASSDIRLSLDYDAAEL
                     CEEQIEAFGNYYLSALRAMADDPSARYDLHCLLPEDERVRVLYEWNQTHAPDAQTQCL
                     HEPFEAQAARTPDAIALVCEDARLTYDELNRRANRLAHRLLGLGVGPELRVGICLDRS
                     PEMVVAMMSSLKAGGAYLPLDPAYPSERLRFMLEDSRATVVITQRRLLHQLPPHAAHV
                     ICLDEEADESARVSEDNPAVKVWPDNLAYVIYTSGSTGAPKGVAIQHRSAVAFLSWVG
                     KVFAPQDLVGVLFSTSICFDLSVFELFAPLNAGGTVILAENALRLPTLPAAGEVTLIN
                     LVPSALAELLRVDGIPESVRTVNLAGEPLALELVRQTYAKGHVERVFNLYGPSEDTTY
                     STGILLEKDGCEPPPIGRPISNTQVYLLDRQMQPVPVGTPGVLYIGGDGLARGYLNGS
                     QLTAEKFVPHPFSTEPGRRLYNTGDLARYLPDGNLEFLGRIDQQVKVRGFRIELGEVE
                     AVLKQHPAVESAVVLAIDGGPGEKRLVAYVVPEGSETISARELHDSLSEKLPGYMIPS
                     TFITLERLPLTPNGKLNRAALPAPERDGVELRHASMPPRDTLELKLARVWEEVLGVRP
                     VGVLDNFFLDLGGHSILAVRLMAQIEKQFGRSISLPAFLRGATVEQLAAALRREQEAD
                     SPTPLVPINPNGLRPPFFCVHPATGSALCYVHLARHLGPDQPFYGLQAPGLDGDQQPL
                     SDIEEMARHYIDALRTVQPEGPYYLGGHSFGGVVAYEMAQQLGRVGQEIALLAILDTM
                     APSGPAPSPPELDDTMMLCEIAGVIERFLNQNLSVKYEDLKALAFEEQLSILLARLKE
                     IGILPQDAETKHVRGLLQVEKANVTALGRYVPQSYRGKITLFRAAVLHSEDFRSLSLR
                     GFLDPTYGWGGLTTISPEVHIVPGDHITMITEPSAAVLAERLRSPLGVLSVGGWGRPD
                     ASEARSSGVRELRP"
     gene            complement(13075..15132)
                     /locus_tag="VF240_08295"
     CDS             complement(13075..15132)
                     /locus_tag="VF240_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7175268.1"
                     /translation="MRLRSRIRGRERWEVKALRGRPGTAQDLERLLSQRQGVLHVKAN
                     PTSAGVLVTYSTDTPDFDVEALIREGFKQLPPPVASRPSGAKSGSAISGILKSFLPRD
                     QALTPPVLSTVGHSVHLLQGLSFVSIVNTARGSGPGFLRSLGIVSTGAQLLVMTGWSL
                     LLTGVDLYLQYHRKEAWRRLGQATQHDLRDQLITRIEKQDVEFFDRYGTGPLIDLVTK
                     DTAQVGEFVERSGDVIIEKALTIAVAGFFIITSAPSLGLIIFLPLPIIFLSSRFFGGK
                     ITERYARRGETTKQFNQMLENNLAGIADVKSFTAEWEEARRLSECDAALAQHTLGAER
                     AASLQTNFISGLFSTTFVLTAGYAGFITAGGGISLSNFLRLVYWFPQLLRSLTGIEQV
                     TGLYHRADDSAGRLARVLESYPQIQSGPVRLSAEEVRGEVIFEDVSFGYDPSVKVLEN
                     VSFHLRPGETVGIVGPTGSGKSTLLRLLLRFFDVDSGRISLDGNDIRDLDLHNLRSAV
                     SMVSQDVYLFQGTIRENVIFGQRRATEEQILEAMRDAGALDLITSVPGGLDAEVGERG
                     RRLSGGQRQRVAIARALLKLTSGASILALDEATSHLDNETESAFKRSLRKAATGKNVI
                     IIAHRLSTIRSVDRILVLESGRITEAGTHDELLAHEGLYASLWRLQNEDPFGDQLEIR
                     LKR"
     gene            complement(15166..15378)
                     /locus_tag="VF240_08300"
     CDS             complement(15166..15378)
                     /locus_tag="VF240_08300"
                     /inference="COORDINATES: protein motif:HMM:NF028504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5132 domain-containing protein"
                     /protein_id="HEX7175269.1"
                     /translation="MKKTHFFVGLVSGIVLAQHWRFLAKEGIKGGIKAGRAMKEMSQR
                     AMEDMEDIAAEATAEVSEQPPQDPKW"
     gene            complement(15712..15990)
                     /locus_tag="VF240_08305"
     CDS             complement(15712..15990)
                     /locus_tag="VF240_08305"
                     /inference="COORDINATES: protein motif:HMM:NF039340.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6072 family protein"
                     /protein_id="HEX7175270.1"
                     /translation="MPNEPANVNNVVVTGLHVAGEYVFPGGSNLVKGDFQQAALHAGA
                     GILAAALFGPIGLLLVRANSLSVALTDRSLSENLSSAATPSSSSRTAK"
     gene            16304..16897
                     /locus_tag="VF240_08310"
     CDS             16304..16897
                     /locus_tag="VF240_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175271.1"
                     /translation="MRPTILLIGPLCVGKSTVAELLATSLGIPRCSVDDLPMEYFVEL
                     GLDPSRVETLTASQEWLAAYHYLQEFGVRLVERLLPERAGHVIDFGAPYSIYEDDSLL
                     ERVKKAFEPYPNVVLLLPSPDLDESTKVVKARMAAREGHIEIRRLLAGIGGETGVDYE
                     ELYVKHHSNFQLARTVVYTEGKTPEQTRDEIIRSLEL"
     gene            17035..17802
                     /locus_tag="VF240_08315"
     CDS             17035..17802
                     /locus_tag="VF240_08315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX7175272.1"
                     /translation="MQAGNNDVLRFSYGGEEYFAKAYKNKACFRRELYALENFLASEI
                     PVPEVVYKSKSCEGAENGLIVMRKAGGMPLDKIEVRRNEYCYEMGCLLSRIHSLELQT
                     EIEPWIIPAQEIAGQVLAFSEEENIRHRTSGLVEKTFEKFKLSQSVVVSHGDYIERNV
                     LGSDGRITGIVDWECLRAARPEVDLGYCCAFLDIFRNPDEERSFIKGYSLKFDEQLKD
                     EIKLLYKMILARYWQQTGKSQEYQKALSAVKKQLADL"
     gene            complement(17872..19269)
                     /locus_tag="VF240_08320"
     CDS             complement(17872..19269)
                     /locus_tag="VF240_08320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7175273.1"
                     /translation="MLIVDDDPVVVDSLAEMLSTWGFLTDTAADGVAGLERAEEFNPS
                     VVLTDILMPRLDGFGLLRELRRRNPATAVILLTGQGSVETAMRAVQDEGAFYYFEKPI
                     DFGRLRDIVERASVHGVEEGARELSRQSGFGEMVGASAPMQQIYTLVEQVASSSASVL
                     ITGESGTGKELVARTIHKLSPRREQPFVAINCSAIPETLMESELFGHERGAFTGAAGR
                     RQGCFEQADGGTLLLDEIAEMPALLQAKLLRVLEERTLRRLGGSQEILVDVRVLAATN
                     RDPLDAVHSGSFREDLLYRLNVITIEMPPLRRRLDDIALLSEHLIAQLAERHNRPARR
                     LSKAALEALKSHHWPGNVRELRNVIERAVIICSGEAIERHHLAPYPLEQRARARSEDT
                     ITLPVGTPIEEAERQLILRTLQKTDNNKTRAAELLQISLKTLHNKLRLYRERGTMQDF
                     ESKESELTGRATHSD"
     gene            complement(19350..20183)
                     /locus_tag="VF240_08325"
     CDS             complement(19350..20183)
                     /locus_tag="VF240_08325"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX7175274.1"
                     /translation="MTDIEARTTEAGVPLSGRTVMITRARRQAAEFASELERLGARVV
                     ECPTIEIVEPESYAPLDQAVEDLFGYDWIVFTSANGIEHFLRRLELKGMDASALDELR
                     VCAIGGATADRLRDAQIHVDVVPEKFQAEGVYAALESYVGGRAQLDRLNFLIPRAAVA
                     RDHLPRALEAAGARVDVVPAYRTVRAETHDRARVEALLLGGAVDCVTFTSASTVINFA
                     QLFETGDLRNLLDGIAVACIGEITAHTASGYGLHTHVRPGESTVSALALAIAEFFRQA
                     R"
BASE COUNT         3787 a         6341 c         6409 g         3759 t
ORIGIN      
        1 ccgcccgacg aactcctcag gctgctcgaa gcggagggcg aaatgaatgc gtgagcgcgc
       61 gccggcgcga agcgtttgaa gtggcgcgaa acgggtcttc acacccgcgg gaaggaattg
      121 acgatgactc tcaccgaaca acacgtgaag aaatacgagg aggatggctt cctcttcttc
      181 cccgagcact tgtccgccgc ggacatcgaa ccgctgcggg aacaactgcc cgccgtctat
      241 gccgaggact ccgcgcgatg tgtgctggag gagggaggcc gcgtcgtgcg ctcggtttac
      301 gcttcgcaca cgacgaacga gactttcagg cggctgtccg aagacccgcg tctcgtcgcg
      361 aaggcgatga agatactcgg cggcgaagtc tacatccacc agttcaagat caacgccaag
      421 gccgccttcg gcggcgaccg ctgggagtgg catcaggatt acgtcttctg gcgcgaggag
      481 gatgggatgc cgacggcccg tgtcacgaac gtcgccgtct tcctcgacga agtgaacgag
      541 ttcaacggcc cgctgctgct catccccggc tcgcaccggc aggaggtcat cgaagtcgaa
      601 gggcgctcgg aaatccccgc ggcctacgcc gacagcccgg cctggataac gaacctgacg
      661 gcggagctga aatacagtct cccgcgtgac atcgtcgcgg ggctggtcaa acgccacggc
      721 atcgtcgccc cgaaggggcc ggccggctcc gtcctcatct tccatccgaa cctcgtacac
      781 ggctcggttc aaaacatctc accgtttgag cggacaatcg tctgcgtcac ctataacagc
      841 atcttcaaca ctccgccggc actcgaaaag atgcggcccg ctttcctctc gagccgcgac
      901 ttcacgccca tcaggctacg tgatgacgag caccgtgccg cgcgagaaga gaaggagcgt
      961 cgatgaaact ggcgttggcc atgaatgtgc catacgcgcg cgtttacggc ggggcaaatc
     1021 gttcgaaccg cgctttggcc gaagcccttg ccgcgagggg ccacgaggtg catgtcatca
     1081 ccccggccct ggccgtgccg agcaccatca cccgcgagga cttcgtcgcg gggctggcag
     1141 cggagggcgt cgccgtccgc tccgaaggga gcgtggaccg tttcaatctg ggcggggtgg
     1201 aggttcatgc gttggccgaa cagtcacaac tgcgcggcta tctgacgcag gcgctcggcc
     1261 ggttggaacc cgactgggtg ctggtgtcct cggaagaccc ttcgcaaaac ctgctcgacg
     1321 cggcgctgca aacctgtccc tcgcgggtcg tctacatggc cctgacgccg ccaatgctcc
     1381 cgttcgggcc ggtgagcctg tatcccggcg aagcgcgcac cgcactgctc ggccgcgccg
     1441 ctgggatagt ctccatcagc gattatctgg cgcgctacat caaggagtgg gcgggctttg
     1501 agtcgttcgt ctttgtgccc ccgcattacg gcgccggccc gttccccgac ttcgggcgtt
     1561 tcgacgacgg atacgtcctg ctgatgaacg cctgcgccat gaagggcctc cccgttttcc
     1621 tctcgctcgc gcggatgctg ccccgcgtgt ctttcgccgc gctccacggc tacggcacga
     1681 ccgaggcgga ccgtgaggcg ctgaagtcgc tgccgaacgt gtccatcctt gagaacagca
     1741 aaaacctcga cgacattttt cgtcagacgc gcgtgctcct gatgccttcg ctgtgggccg
     1801 aaggctttcc gctcgccgtc atcgacgcga tgttgcgcgg catccccgtc atggccgccg
     1861 cgcacagcgg gctggtcgag gccaagctgg gaaccgacta cctgctcccc gtccggccga
     1921 ttgagcattt cgccgatagc ctcgatgaca acatgctccc gtcgccaatc atccccgaac
     1981 aggatgtcga gccatggcgc gacgcgctgg tcgaactgct cacagacggc gacgtgtacc
     2041 ggcggcagtc ggaggcggcg aggacggccg cgcgcgccta cgtcgcgaga ctcggcgtcg
     2101 agccgctcga agaattcctg cgcggactgg agacgcgccc gcgccccggc ggcatgcggc
     2161 ccgagacacc ggaagcgaaa aaagatgacg ccttcgccag cgccgccgct ctgactcccg
     2221 agcagaaagc cctcctgatg ttgcggctgc gtaaaaaggc gcgggccgcg caggtcgtcg
     2281 agacgggaaa aaactccatc ccgtcggtct cgcgtgacct gaacactccg ctctcattcg
     2341 cgcagaatcg actctggttc cttgatcagt tggagccggg caaccccttc tacaactgcc
     2401 ctatcgccat ccgcctgcga ggccggctcg acgcggcggc gctggccggc gcgctgggcg
     2461 agatcgttcg acgccacgag gtcttgcgga cgacgttcaa gttggtcggc gaccgaccca
     2521 tacaaataat ctcggagcga gagcaggagc tgccggtcgt agacctcagc gggcggcccg
     2581 gtgcggagca agaggcgcgc cgtctcctct acgaagaagc acggcggcct ttcaggctcg
     2641 atgaggggcc gctcctgcgc accacgctga ttcgcatgag tgagcatgag cacgtcctcg
     2701 tcgccgtcgt ccaccacatc gtcgcggacg gatggtctac cggaattttg gtgcgtgaga
     2761 ttaaaacact ctacgagtcg ttctcccgtg gggagtcttc gccgctcgcc gagctacaga
     2821 ttcagtatgc ggatttcgca cactggcaac acgaacatct tcagggcgag cgcatggaga
     2881 agctcatcaa cttctgggag caacaactcg gcggcaacct ccccgtgctc gaactccccg
     2941 ccgaccggcc acgcgcgaac gtccagacct ttgccggcgc gcgccacgca ctcgcgctcc
     3001 cccggtctct caccgaagaa ctcggggcgc tgtgccgggc cgagggcgcg accctgttca
     3061 tgttgctgct gtcggcgttc aaggtgctgc tccaccgcca cacgcggcag gccgacatca
     3121 tcgtcggcgc gcccatcgcc aatcgcaatc gccgggagat cgaagacctc atcgggttct
     3181 ttgtcaacac actggtgctg cgtacagaca tttcaggcga cccaaccttt caggagttgt
     3241 tacgccgcgt ctcacgcgtc acgttggagg catacgacca tcaggacttg cccttcgaga
     3301 aactggtcga ggtcttacaa ccggggcgcg acatgggcca ctcggcgctg tttcaggtga
     3361 tgttcgtgct acagaacgcg cagctcgcgc cgctggagtt gccgggcctc atgctgactc
     3421 ccgtcgatct ccacagcggc acgtcgaagt tcgatttgac attggagatg gtggaggcgg
     3481 aggaagggct gacggcgctg ctggaataca acacggactt attcgacgcc gccaccgccg
     3541 aaggaatgtt ggagcagtat cagacgttgc ttgaggccgt cgtgcgcgag ccgcggtcga
     3601 aagtctcggc cctgcccctg ctctcgccgg aagcggagcg gcggctgcta tcggaattta
     3661 acgatacggc ggttgcgttc ccgccgcctc actgcatcca ccagattttc gaagagcagg
     3721 tcgagctgac tcctgacgcc gtggcgctcg tcttcgagga agaacagttg acctatcgcg
     3781 agctgaaccg gcgcgcgaac caactggcac accacctccg atcttttggc gttgggccgg
     3841 atgtgccggt cggcatctgc atggggcgtt cgattgagac aatcattgcc gtgctcggca
     3901 cgctcaaggc cggcggggct tacctgccgc tcgaccccgt gtacccggcc gagcgtctcg
     3961 ccttcatgct cgaagactgc caggcgagcg tccttctcac tcaaagcgat ttactcgggg
     4021 cgctgccgcc gggccgggcg cgcgtgctgt gcctcgacga cgcgcgcgaa gagttgaatg
     4081 gcgagagcga cgcgaacccg cgtagcggcg tggccgagga gaacctcggt tacatcatct
     4141 acacctccgg ctcgacggga aggccgaaag gcgtctccct cccgcagagg gctttggtaa
     4201 atcttctgcg gtggcattgc gccacgctcc gaccgggcgc gcgcacgctc cagttcgcct
     4261 cgctgagctt cgacgccagc ttccacgaaa tattcgccgc cctgttcacg ggcgggactc
     4321 tctatctgat gcaggaaggt ctgcgccgcg acatgaatgc gctcgccgtg ctcatgcgcg
     4381 atcaggcaat cgagaagatc attctgcccg tcgtcgtctt acaggaattg gccgcggagg
     4441 cgcgcgagcg tgggcacgag ttcccacggc tgaaggaagt gatggcgacc ggcgagcagt
     4501 tgcaagtcaa caaatccgtc atcgattggt tcgacgacat gcagggtggg ccacggggcg
     4561 gcggctgctc gctccacaat cactatgggc cttcggagac acacgtcgtc acgtcctaca
     4621 cgttgccgcc cgcgccgcga aactggccga cgtacccgcc catcggacgc cccgtcgcca
     4681 acacgcagat ttacatcctc gacgcgcaac tcaacccgac gccgacaggc gtgcccggcg
     4741 agctgtacat cggcggggcg aatctggcgc ggggctattt caaccggccc gatttgaccg
     4801 ccgaaaaatt catcccgaac ccgcacggcg acgaagccgg gggccgcctc tacaagaccg
     4861 gcgatctggc ccgctaccgc gcggacggaa acatcgagta cctgtgccgc atcgatcatc
     4921 agttgaaggt gaggggattc cgcgtcgagc cgggtgaggt cgaatcagtg ctcgggtcgc
     4981 acccggcagt ggagaagacg gtcgtgctcc tgcgcgagtt cgcgccgggc gacaggcggc
     5041 tggtcggcta cgtcgtcggc cggcaggggc cgcgaccctc ggccagtgag ctgcgcgaat
     5101 tcctgttgcg gaagttgccg gagtacatga ttccttcggc ctatgtcttt ctggacgcgc
     5161 tcccgctgac cccgaacggg aagctcgacc gcgacgccct gccgatgccc gattcggcgc
     5221 gccccgacgt gggcggcggc ttcgtcgcgc cgcgcacaga gattgagaag tcacttgcgg
     5281 agatttgggc cggagtctta ggcatcgacc tcgtcggcgt caacgacaat ttctttgaac
     5341 tcggcgggca ctctctactg atgattcaga tcgtctcgcg catctcggaa gccctgcacg
     5401 tcgagctgcc gttgaaggac ttctttcaac tcccgaccgt ggcggagcag gccgccgccg
     5461 tcggccgact gatggccgag gggagcgaac agcgcgggat cgaacagttg ttagacgatc
     5521 tggatcagct ttcgccagaa gaggtgcggc gactgctcga tgagagggcc ttgacgcaga
     5581 attgaaccgg agaatgtgag acgtgagcgg tggagaagac cgtccattac ataagggagc
     5641 cagagcttga gtaatacttc tgaacgcatc gcaaatttgt cgcaggaaaa acgtcgcctc
     5701 cttgagcttt tgttactcga agaaaagggg cgcgggcctc gcgacgagcg catccctgcg
     5761 cgcggcgaag tctccccgtc gtcgccgctt tccttttcac agcagcggct ctggttcttc
     5821 gatcaggtga cgccgggcag ccccctccat aatctctcca gcatcctgcg ggccagaggg
     5881 gcgttaaatc tgaacgcatt tgaacagagc cttaacgagt tggttcgcag acatgagtcg
     5941 ttgcggacga cctttccggc ggatgagggc cgcccgcttc agttagtggc cgcgccccgg
     6001 cccctgcaaa tcacagtcaa cgatctcggt gatttaccgg agacagcacg cgaggcggaa
     6061 gctttgcgcg tggctgccga ggagggccgc cgtcccttcg atttggcgcg cggcccgttg
     6121 atgcgcgcct ccgtcctgcg cctcgggccg gaagaccatc tgctcgtttt taccacgcac
     6181 cacatcgtca gcgacgggtg gtcgcagggc gtcatcgtgc gcgagatgtc ggagctttac
     6241 cgttcattcc tcacgggtgc gcaggttcgt ctgccggagt tgccgattac atacgccgac
     6301 ttcgccaact ggcagcggga gacgttgcgg ggcgaggcgc ttgaggccga ccttacctac
     6361 tggaagcggg cactggacgg cgtcgcccca ctcgacctgc cgttggatcg cccgcggcct
     6421 gccgtgccgg acttccgaag cgaggaagaa gtattcgagt tgcccgagca tttatccgag
     6481 gctctcaggg cgctcagccg acaggagggc gtcacgctct acatgacgct cctcgccgcg
     6541 ttcgaggtgt tgctccgacg ctacacggga caaaccgata tcagtgtcgg ctcctctgtc
     6601 gccaaccgca cgcggcgaga gatcgagaac atcgtcggct gcttcgtcaa catcctcgtg
     6661 ttgcgcacag acctctcggg cgacccgaca attggtgaac tgctcggacg cgttcgagag
     6721 gtgtgcctcg gcgcgtatgc ccacgccgag ttgccgttcg agcgcgtggt cggcgaggtt
     6781 cagaccgacc ggaacttaaa tcacaacacg ctgtttcagg tggcgttcat cttgatgagc
     6841 gcgccggccc ccgatctgca actgcccggc ctcagcctga gccgggtgga gataaaccac
     6901 gggctgaccg aatacgatct gagcctgatg ctggccgaag aggggcagag attggtcggg
     6961 cggctcactt acaggtcgga gttgttcggg cgcgacacgg cccggcgcat gctcggccac
     7021 tggcgacgga tactcgaagg cttcgtcgcc gacccgcacc ggcgcctctc cgaattggag
     7081 ctactttcca acgccgagcg ccagcatctc cttcacgatt taaatcaaac ggcaacggac
     7141 tacgaacgtg agatgtgcgt tcacgaagtg ttcgagcgac aggcggagct cgcgccggac
     7201 gcggtcgccg tcgtccacga cggggggcag ttgacctatg gcgagttgaa ccggcgtgcg
     7261 aaccagctcg cgcgacacct gcgctcgctg ggggtcgggc ctgaggtgcg cgtcggcgtc
     7321 tgcgtcgagc ggtcgccgga gatgatcgtg gggctgctgg cgatcctcaa ggccggcggc
     7381 gcctacgtcc cactcgaccc ttcatacccg cttgagcgcc tgaccttcat gctggaagac
     7441 tcggcggcgc ccgtgctctt gacgcaggaa cacctgttgg acgtgctgcc cgccacatgg
     7501 gcctcggtgg tttgcctcga ctcggattgg gaactcgtct cgcggcagag cggcgaagac
     7561 gttcggggcg gggccgcccc tgaaaacctt tgctacgtga tgtatacgtc cggcacgacg
     7621 ggcaggccga aaggcgtcag cgttactcac aggggggtgg tgcgcctcgt cagagaaacg
     7681 aactacgtga atcaaacccc gcatgacacc tttctgcaat tcgcaccaat ctctttcgac
     7741 gcctcgacct tcgaggtctg ggggagtctg ctgggcggcg cgcgtctcgc actggccccc
     7801 gcatccacca gatcgccgga agaactcgcc gagaccatcc gcactcaccg tgtgacgata
     7861 ctgtggctca ccgcggcgct gtttcaccag atggccgagc attaccccga aagcctcttc
     7921 ggcgttcgcc aactgctcgc gggcggcgac gtgttgcagc cctcccaggt gcggaaaatg
     7981 cttcgggggc cggcggagaa tctgctcatc aacggatacg ggccgacgga aaatacgacc
     8041 ttcacctgct gccacgtcat gtccgccggc gacgacctcg aaggcgccgt ccccatcggg
     8101 cggccgatag ccaacacaca ggtttacatc ctcgacgaac acatgaggcc gctgcccatc
     8161 ggcctgcccg gagaactata tatcggcggc gacgggctgg cgcgcgacta cctcaaccgg
     8221 ccgggcttga ccgccgaaaa gttcgtcccg catccgttcg ccgacggccc cggtgagcga
     8281 ctcttccggt cgggtgatcg tgcgcgttac ctagtcgatg gcaacatcga atttctggga
     8341 cgtgcggacg gtcaggtcaa gattcgcggt ttccgcatcg agttggagga agtcgagagc
     8401 gcgctattgc ggcacccttc ggtggggcag tgcgtcgtcg tcgcgcgcga ggagcggccg
     8461 ggcgacaagc gtctcgtcgc ctacgtcgtg tcgggtcttg ggcagcccgc cgagccggaa
     8521 cagttacgcg gctttctgaa actacgcctg cccgattaca tgctgccgtc cgccttcgtc
     8581 gcgctcgaag cactgccgct gacgccgagc ggcaaagtgg atcgtcgcgc cctgccggcc
     8641 cccgagacgc agcgagcgca acccacgcag aacttcgtcc cgccgggcac ccctgccgaa
     8701 agtcttttag ccgacgtgtg ggcgcgtgtg ctcggcgtcg agcgggtggg cgctcacgac
     8761 aactttttcg acctcggcgg ggattcgatc aggagcatcg aagtctgcgc gctggcgaag
     8821 aaggcggggg ttgaagtatc cgtccagcag ttgttcgagt gtcagaccat tcacgaattg
     8881 gcgcgagttg ccttctcgcc cgagcttgtc acgcccgagc cggcgagcgt tcccttttcc
     8941 ctgatctcgg acgatgaccg cctcgccctg ccggaggacg tggaagacgc ttacccgctc
     9001 acgcgccttc aggcgggaat gatttttcac agcggctacg ccggagacgc ggcgacttat
     9061 catgacatct tcagttatca cctgagcggc ccttgcgtgc cggagttgat gcgacgggcg
     9121 acggccgagc tggtcagtcg tcatgccgtg ctgcggactt cctttaacct gacccgttac
     9181 ggcgcggcaa tgcaactcgt ccacagggaa gtggagctgc catttgaagt cgcagacctg
     9241 cgccatctct cggaggcgga gcaggaaacg atgatcggcg agagcatgga gacggaaaga
     9301 gcgaggcgct tcgactggga acaagctccg ctgctgcgcg ttcaactcct tcgccggggc
     9361 gagcatacgt atcaactcac gttgagcttt caccacgcga tcctcgacgg gtggagcctg
     9421 gcctcactcc tgacggaact ttataaccgc tacgtctctc tgctcgaagg agcggacgcc
     9481 gccgccgcgc cgccgcccga aacggccttc cgcgactacg tggcgcttga gttagacgcc
     9541 ctcgaatcgg ccgagcatcg ctcgtactgg gcgggcaagt taaacgacag caccgttatc
     9601 aaacttccgc gacgccccgc gtcgatgctg gcgggccgcg acagacgcgt cagggagttg
     9661 cttgtccctc tgacagccga agagtgcggc gcgctgagga acctcgcgcg ggccgaggga
     9721 gtggcgctca agagcgttct gctggccgcg cacttcagag tgatgagctt actcgccgca
     9781 cagccggacg tgctgaccgg cgtcgtctcg aacgggaggc cggagacggc ggacggcgag
     9841 cgtgtcctcg gcctcttcct gaacacgctg ccgctacgcc tcaaactttc gcggggcaca
     9901 tggcgcgatc tcatccgcca gaccttcgag gccgagggcg aagcactgcc gttcaggcgt
     9961 tacccgctcg ccgagatgca acaggttcac ggtagcggac agcccctttt cgagacctgt
    10021 ttcaacttca cccattacca cgtcttcgag ggcatacagg agtccggtaa ggtgcgtgtt
    10081 ttgggcgggg cgagcgcggc ggaaacgaac ttcaccctgc tgacgaactt caacgtggac
    10141 ccggcctcgt ccgacatccg tctgagtctc gactacgacg cggcggaact ctgcgaggag
    10201 cagatcgagg cgttcggtaa ttactacctc tccgcgctgc gcgcgatggc cgacgaccct
    10261 tcggcacgat acgaccttca ctgtctgctc cccgaagacg agcgcgtgcg cgtgctctac
    10321 gagtggaacc aaacccatgc cccagacgcg caaacacaat gtctccacga accgttcgag
    10381 gcgcaggccg cccggacgcc ggacgccatt gccctcgtct gcgaagacgc gcgactcacc
    10441 tacgacgagt tgaacaggcg agccaaccgg ctggcccacc gcctgctggg cctcggcgtc
    10501 ggcccggagc ttcgagtcgg catctgcctc gaccgaagcc ccgaaatggt cgtggcgatg
    10561 atgagttcac tcaaggccgg gggcgcatac ctgccactcg atcccgcgta cccgagcgag
    10621 cgcctgcgct tcatgctcga agactcacgc gccacagtcg tcatcaccca gcggcgcctc
    10681 ctgcatcaac tacccccaca cgcggcccac gttatttgtt tggacgaaga ggccgacgag
    10741 tccgcgcggg tgagcgaaga caacccggcc gtcaaagtct ggcccgacaa cctcgcatac
    10801 gtcatctaca cttccggctc gacgggcgct ccgaagggcg tggcgataca gcaccgcagc
    10861 gccgtcgcct tcctgtcatg ggtcgggaag gtcttcgcgc cgcaagacct cgtcggggtg
    10921 cttttctcga cctccatctg tttcgatctt tccgtcttcg agttgttcgc gcccttgaac
    10981 gcggggggca cggtcatcct cgccgaaaac gccctgcgat tacccacgct gcccgccgcc
    11041 ggtgaagtga cgctgattaa tctagtccca tcggcgcttg ccgaactgct gcgcgttgac
    11101 ggcatccccg agtcggtgcg gactgtgaat ctggcaggcg aaccgctcgc cctcgaactc
    11161 gtgcggcaga cttacgcgaa gggacacgtc gagcgcgtct tcaacctcta cgggccgtcc
    11221 gaagacacca cctactcgac cggcatcctt ctggagaagg acggctgcga gcctccgccc
    11281 atcggccgac ccatctcaaa cacgcaggtc tacctgcttg atcggcagat gcaacccgtc
    11341 cccgtcggca ctcccggcgt cctgtacatc gggggagacg gcctcgcccg cggctatctg
    11401 aacgggagcc aactgaccgc cgagaagttt gtgcctcatc ccttttcgac cgagccgggc
    11461 cgcaggctct acaacacggg cgaccttgcg cgctacctgc cggacggaaa ccttgagttc
    11521 ctcggacgca tcgaccaaca ggtgaaggtg cgcggattcc gcatcgaact cggcgaggtc
    11581 gaggcggtgt tgaaacagca cccggccgtg gagagcgccg tcgtgctggc catcgacggc
    11641 ggccccggcg agaagcggct ggtggcttat gtcgtccctg aaggctcgga aacgatttcc
    11701 gcccgcgagc ttcatgattc tttgagcgaa aagctccccg gctacatgat tccctcgacc
    11761 ttcatcacgc ttgagagatt gccgctaacg cccaacggca aacttaaccg cgcggcgctc
    11821 ccggcgcccg aacgtgacgg cgtcgaactt cgacacgcat cgatgccgcc ccgcgacaca
    11881 ctcgaactca aactggcgcg cgtctgggaa gaagtgctcg gcgtcaggcc ggtcggggtt
    11941 ctggacaatt tcttcctcga cctcggaggc cactcgattc tggccgtgcg gctcatggcg
    12001 cagatcgaga aacaattcgg ccggagtatt tcactgccgg cgttcctccg cggcgcgacg
    12061 gtcgaacaac tggccgcggc gttgcgccgg gaacaggaag cagattcgcc gacgccgctc
    12121 gtgccgatta acccgaacgg cttaaggccg ccgttcttct gcgtgcaccc ggcgacgggg
    12181 agcgcactgt gttacgtaca cctcgcgcga catctcggcc ccgaccagcc gttctatggg
    12241 cttcaagcgc cgggcctcga cggtgaccag cagccgctct ccgacatcga ggagatggcg
    12301 cgccactaca tcgacgcact gcggacggtg cagcccgaag gcccttatta tctgggtggg
    12361 cattcgttcg gaggggtcgt ggcctacgag atggcgcaac agttgggcag ggtagggcag
    12421 gagatcgcgc tgctggccat cctcgacacg atggcgccgt caggcccggc cccttcgccg
    12481 ccagaactcg acgacacgat gatgttgtgt gagattgccg gggtgatcga gcgcttcctc
    12541 aatcaaaatc tctcggtcaa atatgaagac ctcaaggccc tcgcgttcga ggagcagcta
    12601 tcaatcctac tggcccggct gaaggaaatc ggcatacttc cgcaggacgc cgagacaaaa
    12661 catgtgcgag gtctgctgca agtcgagaag gccaacgtca cggccctcgg cagatacgtc
    12721 ccgcaaagtt accgaggcaa gatcactttg ttccgcgcgg ccgtgctcca cagcgaggat
    12781 ttcagaagtc tatctctgcg cgggttcctc gatcccacgt atgggtgggg cggcctcacc
    12841 acgattagcc ccgaggttca catcgtcccc ggagaccaca ttacgatgat tacagagccg
    12901 agcgccgccg tgttggccga gcgacttcgt tcgcctctcg gcgtgttgag cgtgggcgga
    12961 tggggcaggc cggacgcatc cgaagcacgg tcgagcggcg tgagggaact gcgtccgtga
    13021 cgggccgcga gagccgtgcc cgcggccgtg gacaagcatt tcgtttcccg gccgttagcg
    13081 tttgagtcgg atttccaact gatcgccgaa tgggtcttcg ttctgaagcc gccacaggga
    13141 ggcatagaga ccttcgtggg cgagcagttc gtcgtgggtg cctgcctcgg taatccttcc
    13201 gctttcgagt acgagaatcc tgtcaaccga acgtatcgta gacagccggt gggcgatgat
    13261 gatgacgttc ttcccggtcg cggccttgcg gagagacctt ttaaaggcgg actccgtttc
    13321 gttgtcgagg tgggaggtcg cctcgtcgag ggcgaggatg gacgccccgc tggtgagctt
    13381 gaggagggcg cgggcaatag ccacgcgctg cctctgcccg ccggataatc gccgcccgcg
    13441 ctcgccgact tcggcgtcca aaccgccggg cacggacgtg attaaatcga gtgcgccggc
    13501 gtcgcgcatc gcttcgagaa tttgctcttc cgtcgcacgt ctctggccga agatgacgtt
    13561 ctcccgaatg gttccctgaa acaggtagac gtcctgactg accatgctca cggcagacct
    13621 caggttgtgg aggtccagat cgcggatgtc attgccgtcc agcgatatcc gcccggagtc
    13681 cacgtcgaag aagcgcagga gcaggcgcag cagcgtgctt ttgccggagc cggtcgggcc
    13741 gacgatgccg acggtctcgc cgggccggag gtggaacgag acgttttcaa gcactttgac
    13801 ggaagggtca tagccgaagg aaacgtcttc gaagatcacc tcgccacgca cttcctccgc
    13861 cgacaggcga accggcccac tctggatttg cgggtaagat tccagcaccc gtgccagccg
    13921 cccggccgaa tcgtcggccc tgtgatacag gcccgtcacc tgctcgatgc ccgtgaggga
    13981 gcgcagcagt tgcgggaacc agtagacgag ccggaggaag ttgcttaaag agatgccgcc
    14041 cccggcggtg atgaatccgg cgtagccggc cgtcaagacg aacgtggtgg agaagaggcc
    14101 gctgatgaag ttcgtctgta gggaggcggc ccgctccgcc cccagagtat gttgagcgag
    14161 cgcggcgtcg cactcgctca ggcggcgcgc ttcctcccac tccgccgtga agctcttgac
    14221 atcggcaatg cccgccagat tgttctccaa catctgattg aattgcttgg tcgtttcccc
    14281 tcgtctcgcg tagcgctcgg taatcttccc cccgaagaag cgggacgaca gaaagatgat
    14341 tggcagtggg aggaagatga tcaggccgag gctcggcgcg gaagtgatga tgaagaagcc
    14401 ggccacggcg atggtcagcg ccttctcgat gatcacgtcg ccgctccgct cgacgaactc
    14461 cccaacctgc gcggtgtctt tggtgacgag gtcgatgagt gggcctgtgc cgtagcggtc
    14521 gaagaattcc acgtcctgct tttcgatgcg ggtgatgagt tgatcgcgca ggtcgtgctg
    14581 cgtcgcttgc cccagccgcc gccacgcttc cttccggtga tactgaaggt agagatcgac
    14641 gccggtcagc agcagagacc aacccgtcat caccagcagt tgggcgcccg tgctgacaat
    14701 gcccagagac cgcaggaagc ccggcccgct gccacgggcc gtgttcacta tcgagacgaa
    14761 tgacagacct tgcaggaggt gaacggagtg gccgacggta gataagacgg ggggcgtcag
    14821 cgcctgatcg cgcgggagga atgatttgag tatgccggag atcgccgacc cggacttcgc
    14881 gcctgaaggc cgcgacgcga ccgggggcgg gagttgcttg aagccctccc ggatgagagc
    14941 ctccacatcg aagtcaggcg tatcggtcga gtatgtgacc agcaccccgg cggaagtggg
    15001 gttagcttta acgtggagca cgccctgacg ctgactgagg aggcgctcca ggtcctgcgc
    15061 ggttccgggc ctcccacgta gagccttgac ctcccagcgc tcgcggccgc ggatgcggga
    15121 gcgcaggcgc attcctaatg agtcatgagt gcccatcccg ccggatcacc acttcgggtc
    15181 ctgaggtggc tgctcggaaa cttccgcggt agcctcggcc gcgatgtctt ccatgtcttc
    15241 catggcccgc tgcgacatct ctttcatcgc ccgcccggcc ttaatgccgc ccttgatgcc
    15301 ttccttggcc agaaacctcc agtgctgggc aagcacaatg cccgagacga ggcccacaaa
    15361 aaaatgtgtc tttttcataa ttgttgaaag cgtccaggtg atttcggagc tgactcgaag
    15421 atactaatct ttgttggagg gggcgggtta accctgacga tgaggcgagg tctcccccat
    15481 tctgagggag gggactgacc aaatcggcat cgatgcgccg ccgcgtccgc tatatcgagt
    15541 tgaccctccg agcaagccgc ggggcgaccg gcggcgcctg aacctggcaa ttcaggaatt
    15601 caacgactct tgcgcctcac ctctcgattt tatagacgac tccatattgg cgtacgcccg
    15661 cagtcagcgg cgtacgccaa tgagccgtta ccgacgcgtt cggtcagtct cttactttgc
    15721 tgtccgagac gaggaagacg gggttgccgc cgagctgagg ttctcactca gactgcggtc
    15781 ggtcagggcc acgctcagtg agttggccct gaccagaagc aggccgatgg ggccgaacaa
    15841 agctgcggca aggattccgg ctccggcgtg aagcgcggcc tgctggaagt ctcccttgac
    15901 gagattgctt ccgccgggga atacatattc gccggccacg tgtaaaccag taactacaac
    15961 attattaacg ttcgcgggct cgttaggcat ctccaggtct cctttgtggt tcggtcaacc
    16021 acggtgtatc aacatgaggg ttgagggttc ttcatctccg agaatgtaaa gcaaaattga
    16081 agttcaattt gaaataactt tatccccgtt cattatttta tgcaaggatt tttgtaaccc
    16141 ggcgagcgcc gcgccgaagt cacagcagaa cctttgcagt cggggttatg tgatagactt
    16201 tgtaatagct tgattcatag acgcgcccct ttgcggccgt tcaaattccg cggccgccgc
    16261 gccccactcc ggggctttcg agcacacgta gaagtgagct tccatgcgcc ccaccattct
    16321 gctcatcggc ccgctctgtg tcggaaagag caccgtcgct gaactgctgg caacgagcct
    16381 cggcatccca cggtgctccg tagatgatct gccgatggaa tacttcgtcg aactcgggct
    16441 ggacccttcg cgggtcgaga cgttgacagc gtctcaagag tggctggcgg catatcacta
    16501 cttgcaggag ttcggggtac gtctggtgga gcgtctgctg ccggagcgcg ccggccatgt
    16561 catcgacttc ggcgcgcctt actcgatcta cgaagacgac tcgctgctgg agcgggtgaa
    16621 aaaagccttt gagccttacc ccaacgtcgt cctcctcttg ccatcgcccg acctcgacga
    16681 gtccacgaaa gtcgttaaag cgcgcatggc ggcgcgcgaa ggacatatcg aaattcgccg
    16741 actgctggcc ggtatcggtg gggaaaccgg agttgattac gaagagttgt acgtgaagca
    16801 tcattctaac ttccagttgg cgagaactgt cgtctacacg gaagggaaga ctcctgaaca
    16861 gacacgcgac gagattatcc gaagcctcga actctaaaag cgcgcgcgtc ccggaggggc
    16921 gcgtcctacc cggggttgag agacgcgacc acaagcccat aacgttttcc cggcaggtct
    16981 atgatgacga tacccaaaag cgtctgtcgc agaatactgt cggagaggcg agacgtgcag
    17041 gcgggcaata acgacgttct ccggttctcg tatggcggcg aagaatactt cgccaaggca
    17101 tataagaaca aagcttgctt ccggcgtgag ctgtacgcgc tcgaaaattt cttagcgtcc
    17161 gaaatcccgg tgccggaggt cgtctataaa tcaaagtcgt gtgagggagc ggagaacggg
    17221 ctgattgtaa tgaggaaggc gggggggatg ccgctggata aaatcgaagt acgtcgcaat
    17281 gaatattgtt atgaaatggg ttgtctgctg tccagaatac attcgcttga actacagact
    17341 gagatagaac cgtggatcat cccggcgcag gagatcgccg gacaggtgct cgcgttttct
    17401 gaggaggaaa atatccgaca ccgaacctct gggctggttg aaaaaacgtt tgagaagttc
    17461 aaactctcgc aaagtgtcgt cgtctcccac ggcgattaca ttgaacgaaa cgtacttggt
    17521 tcagatggcc ggatcacggg catcgtggat tgggaatgcc tcagagcggc gcgcccggaa
    17581 gttgacctcg gatactgctg tgccttcctc gacatattca ggaatccaga cgaggagcgc
    17641 agctttatta aaggttacag cctgaagttt gacgagcaat tgaaagatga gatcaagctg
    17701 ctctacaaga tgattttggc gcgttactgg caacagactg ggaagagtca ggagtatcaa
    17761 aaagccttgt ccgccgtcaa aaaacaatta gctgaccttt aaacctcacg tgccttttta
    17821 tctcggagcc gtcgcgaaaa aatgaaggag ctggctcggg tcttacgccg gctagtccga
    17881 atgtgtggcc cgacccgtaa gttctgactc cttcgattca aagtcttgca tggtcccgcg
    17941 ctcccggtag agacggagct tgttgtggag tgtcttgagg ctgatctgga ggagttcggc
    18001 tgcgcgcgtt ttgttgttgt cagttttctg gagcgtgcgc aggatcagtt gccgctcggc
    18061 ctcttcgatg ggagtgccga cggggagcgt gatcgtgtcc tcgctgcgcg cgcgcgcgcg
    18121 ttgttcgaga gggtaaggcg cgaggtggtg acgctcgatt gcctcgccgg agcagatgat
    18181 gacggcgcgc tcgatgacgt tgcgtagctc gcggacgttg ccgggccaat ggtgcgactt
    18241 gagggcttcg agggcggcct tgctgagtcg gcgtgcgggc cggttgtggc gctcggcgag
    18301 ttgggcgatg aggtgttcgg agaggagcgc gatgtcgtcg aggcgccggc gcagcggggg
    18361 catctcgatg gtgatgacgt tgagacggta gagcaggtct tcgcggaacg agccggagtg
    18421 gacggcgtcc aacgggtcgc ggttcgtggc ggcgagcacg cgcacgtcca cgagaatctc
    18481 ctgtgagcct ccgagccggc gcaacgttcg ctcttcaagc acgcgcagga gcttggcctg
    18541 aagcagggcc ggcatctccg cgatctcgtc gagcagcagg gtgccgccgt cggcctgctc
    18601 gaagcagcct tgccggcgtc cggccgcgcc ggtgaacgcc ccgcgctcgt ggccgaacag
    18661 ctccgactcc atcagggtct cggggatggc cgagcagttg atggcgacga agggttgctc
    18721 ccggcgcgga ctgagcttgt gaatagttcg ggcgaccagc tccttgcccg tccccgactc
    18781 acccgtgatg agaacggaag cggaggagga ggcgacctgt tcgacgagcg tgtagatctg
    18841 ctgcatcggc gccgacgcgc cgaccatctc tccgaatcct gactgcctcg ataattcgcg
    18901 cgcgccctcc tcgacgccgt gtacggacgc gcgctccacg atgtcgcgca gcctcccgaa
    18961 gtcaatcggc ttctcgaaat agtaaaaagc gccctcgtcc tgtacggcgc gcatggcggt
    19021 ctcgaccgaa ccctgtccgg tgagcaggat gacggccgtc gcggggtttc gccgccgcag
    19081 ctcacgcagg agtccgaacc cgtcgaggcg gggcatcagg atgtcggtga gcacgacgga
    19141 ggggttgaac tcttcggcgc gttcgaggcc ggcgacgccg tcggccgccg tgtcggtcag
    19201 gaagccccag gtcgaaagca tctcggcgag gctatcgacc accaccgggt catcatcaac
    19261 aatcagcacg cgccccttac tcatagttgg ggagtgtatc acatcgacaa ggggttcatt
    19321 ctcgcggcag gagagagtgc gcggagactt taccgggcct gccggaagaa ctccgcaatg
    19381 gccagcgcga gggcggagac ggtggactcg ccgggtcgga cgtgcgtgtg cagcccgtag
    19441 ccggatgccg tgtgggcggt gatctcgccg atacaggcga cggcgatgcc gtcgagcaga
    19501 ttgcgaaggt ctcccgtttc aaacagctgg gcgaagttga tgacggtgga agcactggtg
    19561 aaggtgacac aatcgacggc gccgccgagc agaagggctt cgacacgcgc gcggtcgtgc
    19621 gtctcggcgc ggacggtgcg gtaggccggg acgacatcga cgcgcgcccc ggccgcttcg
    19681 agcgcacgcg gcaggtgatc gcgcgccact gccgcacggg ggattaggaa gttgagcctg
    19741 tccaactgag cgcgcccccc gacgtagctt tccagcgccg cgtagacccc ttcggcctga
    19801 aacttctcgg ggacgacatc gacgtgaatc tgcgcgtcgc gcagccggtc ggcggtcgcc
    19861 ccgccgatgg cgcacacgcg caactcgtcg agcgcgctcg cgtccatgcc cttcagctca
    19921 agacggcgga gaaaatgttc gatgccgttg gcgctcgtga acacgatcca gtcgtagccg
    19981 aagaggtctt ccacggcctg atccagcggg gcgtatgact ccggctcgac aatctcgatg
    20041 gtgggacatt cgaccacacg cgcgccgagg cgttccagct cactcgcgaa ctcagcggcc
    20101 tgccgccgcg cgcgcgtaat catcaccgtg cgccccgaca gcggaacgcc ggcctcagtt
    20161 gtacgtgctt caatgtccgt catctttgta ggggcaggtc ttgctcgtag taggacaagg
    20221 cagtagcccg accgtgaggg agggcgcggg gaaaggatga aggatgaagg atgaaggatg
    20281 aaataattgc tcccac
//
LOCUS       DASVES010000212         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_290393, whole genome shotgun sequence.
ACCESSION   DASVES010000212 DASVES010000000
VERSION     DASVES010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290393"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..>2444)
                     /gene="uvrA"
                     /locus_tag="VF240_08330"
     CDS             complement(300..>2444)
                     /gene="uvrA"
                     /locus_tag="VF240_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7175275.1"
                     /translation="FETCFIEGHGQAFVQTAEGSPRALKFSERFECKYDGTIYAEPEP
                     RLFSFNNPYGACPTCQGFGNTIGLDLDLVIPNPLLSLSEGAVEPWTKPQYDWARTELR
                     RFARQEDISMDVPFQELTRQQQRAVVEGKGQWGGVRGFFTWLETKKYKLHVRVFLSRY
                     RGYTLCPDCGGGRLRQEARDVRVGGKTLPEICVLSITDAARFFESLELTPEQTAIADK
                     VLFEVRRRLKFLLDVGLEYLTLERLASTLSGGEAQRIQLATNLGSSLVGALYVLDEPS
                     IGLHPRDSERLIRLLHDLRDIGNTVLVVEHDEEMMRAADHILDIGPAAGELGGRVVYE
                     GDFAGLVKSGATLTGKYLRGETEIKEPKQRRAPKKKHLSVRAAAEHNLKGIDVDIPLE
                     MLTVITGVSGSGKSTLVHDVIYAGIKKQRGEWQGKVGAFKELAGTQHVDEVILVDQSP
                     IGRTPRSNPVTYIKVYDAVREVFSQTRDAQGRGFDPSHFSFNVPGGRCDVCEGDGTVT
                     VEMQFLADVELVCEECKGTRFKPQVLDVRYRGKSIADVLNMTVREAMTFFRDTNKIAN
                     RLRVLDDVGLGYLRLGQSATTLSGGEAQRVKLAAHLSKRAGARTLYIFDEPTTGLHFD
                     DINKLLAAFRTLVEAGGSLLVIEHNLDVIKTADWVIDLGPEGGEGGGRVVAAGTPEQM
                     MKTPASHTGRFLKTHLARSNGHRAERHATEAG"
BASE COUNT          397 a          739 c          878 g          430 t
ORIGIN      
        1 gaggataaac aaaagcgtga caatgatcct ttgtgctttg ctcacgggga tatctccttc
       61 tcacttcgct ggagttgtgg atggctgcga aagaaaccga acccgccctg gaatgtctgt
      121 ggcaagttat acgacgggcg cgcggtgggg cggcgtaaaa gtgttgtaaa aagccgaact
      181 gggagagagc gggcgactcg cagacagtac cgcgagcggt agcgagcggg tctcgtgtgc
      241 gcgagacaca acccgctcgc taccgctcgc ggtactgtct ccgacgtggc gcgcgacgct
      301 taaccagctt cggtggcgtg ccgctcggcg cggtggccgt tggagcgggc gaggtgagtc
      361 ttgaggaagc ggccggtgtg agaggcggga gttttcatca tctgctcggg cgtgccggcg
      421 gcgacgacgc ggccgccgcc ctcgccgccc tcggggccga ggtcgatgac ccagtcggcc
      481 gtcttgatca cgtcgaggtt gtgctcgatg acgaggagcg agccgccggc ctcgacgagc
      541 gtgcggaagg cggcgaggag tttgttgatg tcgtcgaagt gcagtcccgt ggtcggctcg
      601 tcgaagatgt agagcgtgcg cgcgccggcg cgtttcgaca ggtgcgcggc cagcttgacg
      661 cgctgcgcct cgccgcccga gagcgtggtc gccgactggc ccagacgcag atagccgagt
      721 ccaacgtcgt ccaagacgcg caggcggttg gcgatcttgt tcgtgtcgcg gaagaaagtc
      781 atcgcctcgc gcaccgtcat gttgagcacg tcggcgattg atttgccgcg gtagcgcacg
      841 tccaggacct gcggcttgaa gcgcgtgccc ttgcactcct cgcagaccag ctccacgtcg
      901 gcgaggaact gcatctcgac ggtgacggtg ccgtcgccct cgcacacgtc gcagcgtccg
      961 ccggggacgt tgaaggagaa gtgcgacggg tcgaagccac gcccttgcgc gtcgcgcgtc
     1021 tgcgaaaaga cctcgcgcac cgcgtcgtag accttgatgt aggtgacggg gttcgagcgc
     1081 ggggtgcgtc cgatgggcga ctgatcgacg aggatgacct catcgacgtg ctgcgtgccc
     1141 gccagctcct tgaaggcgcc gactttcccc tgccactcgc cgcgctgctt cttgatgccg
     1201 gcgtaaatca cgtcgtgcac gagcgtggac ttgcccgagc cggagacgcc cgtgatgacc
     1261 gtgagcatct ccagagggat gtccacgtcg atgcctttaa gattgtgctc ggcggcggcg
     1321 cgcacggaga ggtgcttctt cttcggcgcg cgccgctgct tcggctcctt gatctcggtc
     1381 tcgccgcgca ggtatttgcc ggtgagggtg gcgccggatt tgacgaggcc ggcgaagtcg
     1441 ccctcgtaga ccacgcggcc gccgagttcg ccggcggcgg ggccgatgtc gaggatgtgg
     1501 tcggcggcgc gcatcatctc ctcgtcgtgc tcgacgacga ggacggtgtt gccgatgtcg
     1561 cgcaggtcgt ggagcagacg gatgaggcgc tcactatcgc gcgggtgcag gccgatggaa
     1621 ggctcgtcga ggacgtagag cgcgccgacg agcgaggagc cgagattggt ggcgagctgg
     1681 atgcgctgcg cctcgccgcc cgagagcgtg gaggcgaggc gttcgagcgt caggtactca
     1741 aggcccacgt cgaggagaaa cttcaggcgg cgtcgcacct cgaagagaac cttgtcggcg
     1801 atggccgtct gctcgggcgt caactccagc gactcgaaga agcgggccgc gtcggtaatc
     1861 gagagcacgc agatttccgg cagggtcttc ccgccgacgc gcacgtcgcg cgcctcctgc
     1921 cgcaagcgcc cgccgccgca gtcggggcag agcgtatagc cgcgataccg ggagaggaag
     1981 acgcggacgt ggagtttgta cttcttcgtc tccagccacg tgaagaagcc gcgcacgccg
     2041 ccccactgtc ccttgccctc gacgacggcg cgctgctgct gccgcgttag ctcctggaag
     2101 ggcacgtcca tcgaaatgtc ttcctgtcgg gcgaagcggc gcagctccgt gcgcgcccag
     2161 tcgtactgcg gcttcgtcca cggctccacc gcgccttcgg agagcgaaag cagcgggttg
     2221 gggatgacca agtcgaggtc gaggccgatg gtgttgccga agccctgaca ggtggggcag
     2281 gcgccgtagg ggttgttgaa gctgaacaga cgcggctcgg gttcggcgta gatggtgccg
     2341 tcgtacttgc actcgaagcg ttcggagaat ttcagggcgc gcgggctgcc ctcggccgtc
     2401 tggacgaagg cttgaccgtg cccctcgatg aagcaggtct cgaa
//
LOCUS       DASVES010000213        14270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_980385, whole genome shotgun sequence.
ACCESSION   DASVES010000213 DASVES010000000
VERSION     DASVES010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14270
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980385"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VF240_08335"
     CDS             complement(<1..1526)
                     /locus_tag="VF240_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEX7175276.1"
                     /translation="MRLVITEKPSMGRDVAAALGATRRGDGFIEGQADIVTWCVGHLV
                     ELDDPDSYDPKLERWRLEDLPFIPEKFKYHPSQRTADQFKVIKSLLGREDVTAVVNAA
                     DAGREGELIFDLVYTLAGCRKPVERLWISSLTREAILDGFRRLKGASEYKGLRESAHA
                     RQQADWLVGINATRAQTIRARRAGHEGVYSLGRVQTPTLALIVERDREISEFVPTDYF
                     EVVADFRAPSGAYRGLWFGKDGTRFDKKEDADAIVAKVSGKTGAVAKVEKKKATERPP
                     LLYDLTTLQRAAGVRYGFTATRTLELAQTLYEKKFLTYPRTSSRHLSADVGKELRAHV
                     EAARVGPYVKFIEEILARDKITLTSRHMDDKKVTDHHAVIPTKQRIEPSALSPDEKRI
                     YDLVARRFLAAFYPDAEIERTTVVTEAEGERFITRGAVVLVAGWREVDPPAKDLKKSG
                     DDEEETDAELPPLKAKDSAEVTHAEALAKQTKAPPRYSESALLGAMETAGRKVEDEEL
                     R"
     gene            complement(1821..2492)
                     /locus_tag="VF240_08340"
     CDS             complement(1821..2492)
                     /locus_tag="VF240_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX7175277.1"
                     /translation="MNKVTCKLSAVALALMFSAAVAATAQDAPKYEELPNFHQVNAHL
                     YRGAQPRAGGVRRLPALGVRTVVNLRAADENSHAEEAEARAAGLQYFNVPLPPYARPD
                     DEQIERALALIDAPENRPVFVHCKYGEDRTGTVVAAYRISREGWTYKRAAVEAEQQGM
                     SRMQFEMKDFIADYYEKTVSGGAGGGGHRARHVAGATASVTRRTLEKSYQVAQKGLRR
                     VGRAF"
     gene            2790..4247
                     /gene="gndA"
                     /locus_tag="VF240_08345"
     CDS             2790..4247
                     /gene="gndA"
                     /locus_tag="VF240_08345"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX7175278.1"
                     /translation="MTTPQTNAHRAEFGVIGLAVMGSNLALNIEEHGFPVAVWNLETE
                     RVDEFLGGAGAGRRFTGTKTFEQLVAALERPRRILMMIKAGAPVDMTIERLRPLLDPG
                     DILIDGGNSWFKDTQRREAACREAGLNFVGMGVSGGEEGARRGPSLMPGGTAEAYERL
                     RPVLEAIAAKTDSGACVTHVGPDGSGHFVKMVHNGIEYGDMQLIAEAYDILRKCLGLE
                     AAELADIFAEWNRGPLESFLVEITSQIFAVRDEETGRALVDLVSDKAGQKGTGKWTAQ
                     VALDLGVPVPTIAAAIDARVLSSMKDERVRASALIEGPRAGAPDGVRAEFVAAVHDAL
                     YASKVCAYAQGMSLIRAASAEYKWETNARELARIWKGGCIIRARFLDKVMRAYERAPG
                     LANLLLDEDIGRSLRESQAAWRRTVAAAVRAGVPVPATSASLAYFDSYRAADLPQNLT
                     QAQRDFFGAHTYERVDKPDAGFIHTDWAGLSGPTD"
     gene            complement(4250..4660)
                     /locus_tag="VF240_08350"
     CDS             complement(4250..4660)
                     /locus_tag="VF240_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175279.1"
                     /translation="MALATDSVSTAQSPRPLPNPVLVFIGQEQYEANGKQWLRYRYVV
                     DNFASYPTDFFAAAPELPPCGLNTKASRTWVDVYEQSGKRLNGFCAFGKPDDLNKVWF
                     SLEPGVVPPSWVYIELNDRKTNTKYKSNLADTTL"
     gene            complement(4967..5566)
                     /locus_tag="VF240_08355"
     CDS             complement(4967..5566)
                     /locus_tag="VF240_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX7175280.1"
                     /translation="MNKKKIELGIRGEKLSPGDHVAYFWENEKDFAEGVKFLEIGLRG
                     EDHCVIFGFEEANEKVCQVLIGHGFNVSALEAAGRLTLLGGESDGDLIMANIGASFER
                     ALEGGAKLIRLLGNIGWGRANWPSERDILAFEAKVTGAAKAFPSVIVCMYDVNNLSGN
                     VIVHGAYETHPLTFCRNMLRENPYYVGIGEFMESLPKMI"
     gene            complement(5938..6417)
                     /locus_tag="VF240_08360"
     CDS             complement(5938..6417)
                     /locus_tag="VF240_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7175281.1"
                     /translation="MEGKIHQLNCSQGGVPKTPVPDAVLTETGLTGDKVAYPKIHGGP
                     ERALCLFPLELIQRLQSEGHPIFPGSVGENVTVEGLDWPSLAPGSRLALGDEVVIEIS
                     SYTTPCGTIKKSFAGGDFARISQKTHPGESRLYARVIRTGRLAVGQSVRLLNGGSAE"
     gene            complement(6538..6717)
                     /locus_tag="VF240_08365"
     CDS             complement(6538..6717)
                     /locus_tag="VF240_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175282.1"
                     /translation="MSDKDRKKKDGRNKDKKNQAARQADNTRKEKAIAPQQAKDASNL
                     TDREAGAATSNRKKR"
     gene            complement(6762..7433)
                     /locus_tag="VF240_08370"
     CDS             complement(6762..7433)
                     /locus_tag="VF240_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175283.1"
                     /translation="MTLKKVGVGGFASNTGKTTLVCELLRAFPGWEAIKVTRGHYRSC
                     GKDPHVCCVSHLLSDEPLVLSERARTDTKGKDTGRYWEAGASDVHWVVATDRQVERGI
                     KEALGRVASAGVIIEGNSFSQFVEVDFFVMVAGPAAKIKPTARRALAKADALYLPGLA
                     GACADELTRREIAELATRQGMADAPPLYAPESFADLVDLIRASLETESTLARQACWPA
                     VASQL"
     gene            7548..7889
                     /locus_tag="VF240_08375"
     CDS             7548..7889
                     /locus_tag="VF240_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175284.1"
                     /translation="MRPNEDLIEEIARALERCSDAATRGESSATVAREWAAAGFDSAE
                     DVEDWLGARCFSAAGAKALDDAGLTPEQAAIRTREGATDYEDTIGFKVSNGDLSTDAA
                     RRIATNSFWNT"
     gene            8146..9612
                     /locus_tag="VF240_08380"
     CDS             8146..9612
                     /locus_tag="VF240_08380"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX7175285.1"
                     /translation="MAKLHELRFDNTYARLPPAFYSKLAPTPLAEPHLVSFNPAAAEL
                     IGLDASEAERPEFVEAFGGKSLPRGAEPLAMLYAGHQFGAYVPQLGDGRAILLGEVRG
                     PAGDKWDLHLKGSGPTPYSRGFDGRAVLRSTVREYLCGEALHGLGVPTTRALCIVGSS
                     EPVVRERVESAAALVRLSPSHVRFGTFEVFHRRAQHAHVRQLADYVIGQHFPELSGLP
                     DGHARFFSEVATRTARLIAMWQAHGFAHGVMNTDNMSILGLTIDYGPFGFIDDFDPGY
                     VCNHSDYAGRYAFDQQPAVGLWNLTRLAEALSTLLAPEQIESALQLYPAAFANAYADL
                     MRRKLGLREERPADAQLVARLLELLQRGHVDYPRFFRALGDFQEAPGATNETLRVMFD
                     SPADFDAWAADYRARLSEERSEPAARKALMDSVNPKYVLRNYLAQNAITRAEGRDYSE
                     IQRLLELLSDPFAEHPGMEDYAAPPPAWGRGLVLSCSS"
     gene            10183..10335
                     /locus_tag="VF240_08385"
     CDS             10183..10335
                     /locus_tag="VF240_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175286.1"
                     /translation="MAVKAATDWGRPVRGLSENVAAFTDFFSYALVRRARAVEHQTPK
                     RQGSGL"
     gene            10299..10381
                     /locus_tag="VF240_08390"
     tRNA            10299..10381
                     /locus_tag="VF240_08390"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10332..10334,aa:Tyr,seq:gta)
     gene            10386..10475
                     /locus_tag="VF240_08395"
     tRNA            10386..10475
                     /locus_tag="VF240_08395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10419..10421,aa:Ser,seq:cga)
     gene            10487..10570
                     /locus_tag="VF240_08400"
     tRNA            10487..10570
                     /locus_tag="VF240_08400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10520..10522,aa:Ser,seq:gct)
     gene            10575..10650
                     /locus_tag="VF240_08405"
     tRNA            10575..10650
                     /locus_tag="VF240_08405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10611..10613,aa:Arg,seq:gcg)
     gene            10676..10750
                     /locus_tag="VF240_08410"
     tRNA            10676..10750
                     /locus_tag="VF240_08410"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10709..10711,aa:Gln,seq:ttg)
     gene            10762..10835
                     /locus_tag="VF240_08415"
     tRNA            10762..10835
                     /locus_tag="VF240_08415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10796..10798,aa:Leu,seq:tag)
     gene            10843..10932
                     /locus_tag="VF240_08420"
     tRNA            10843..10932
                     /locus_tag="VF240_08420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10877..10879,aa:Leu,seq:gag)
     gene            10943..11016
                     /locus_tag="VF240_08425"
     tRNA            10943..11016
                     /locus_tag="VF240_08425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10977..10979,aa:Leu,seq:taa)
     gene            11033..11106
                     /locus_tag="VF240_08430"
     tRNA            11033..11106
                     /locus_tag="VF240_08430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11067..11069,aa:Leu,seq:caa)
     gene            11120..11211
                     /locus_tag="VF240_08435"
     tRNA            11120..11211
                     /locus_tag="VF240_08435"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11153..11155,aa:Trp,seq:cca)
     gene            11225..11309
                     /locus_tag="VF240_08440"
     tRNA            11225..11309
                     /locus_tag="VF240_08440"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11259..11261,aa:Glu,seq:ttc)
     gene            11406..11479
                     /locus_tag="VF240_08445"
     tRNA            11406..11479
                     /locus_tag="VF240_08445"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11440..11442,aa:His,seq:gtg)
     gene            11525..11597
                     /locus_tag="VF240_08450"
     tRNA            11525..11597
                     /locus_tag="VF240_08450"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11558..11560,aa:Asp,seq:gtc)
     gene            11604..11680
                     /locus_tag="VF240_08455"
     tRNA            11604..11680
                     /locus_tag="VF240_08455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11638..11640,aa:Lys,seq:ctt)
     gene            11691..11767
                     /locus_tag="VF240_08460"
     tRNA            11691..11767
                     /locus_tag="VF240_08460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11725..11727,aa:Pro,seq:tgg)
     gene            11785..11860
                     /locus_tag="VF240_08465"
     tRNA            11785..11860
                     /locus_tag="VF240_08465"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11822..11824,aa:Gly,seq:gcc)
     gene            11872..11948
                     /locus_tag="VF240_08470"
     tRNA            11872..11948
                     /locus_tag="VF240_08470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11908..11910,aa:Arg,seq:tcg)
     gene            11975..12048
                     /locus_tag="VF240_08475"
     tRNA            11975..12048
                     /locus_tag="VF240_08475"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12009..12011,aa:Cys,seq:gca)
     gene            12516..13079
                     /locus_tag="VF240_08480"
     CDS             12516..13079
                     /locus_tag="VF240_08480"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7175287.1"
                     /translation="MSLNGRVLLLNFSYEPLGTVGVARAMCLVMRGAVFVEEYEPGRV
                     LRSPSREFPVPSVVRLRAYVNVRRRRQTAGMRRLRIFVRDRFRCQYCGRKQEASKLSL
                     DHILPRSRAGRSGPENLVTACVRCNTRKGNRTPEEARMPLLTAERALTVGLDRVMLCH
                     YAESRPEWRKYMFMAEAEADEHELRRA"
     gene            13152..13223
                     /locus_tag="VF240_08485"
     tRNA            13152..13223
                     /locus_tag="VF240_08485"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13185..13187,aa:Phe,seq:gaa)
     gene            13246..13318
                     /locus_tag="VF240_08490"
     tRNA            13246..13318
                     /locus_tag="VF240_08490"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13279..13281,aa:Asn,seq:gtt)
     gene            13330..13403
                     /locus_tag="VF240_08495"
     tRNA            13330..13403
                     /locus_tag="VF240_08495"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13364..13366,aa:Val,seq:tac)
     gene            13590..13663
                     /locus_tag="VF240_08500"
     tRNA            13590..13663
                     /locus_tag="VF240_08500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13624..13626,aa:Thr,seq:cgt)
     gene            13676..13751
                     /locus_tag="VF240_08505"
     tRNA            13676..13751
                     /locus_tag="VF240_08505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13712..13714,aa:Arg,seq:tct)
     gene            13766..13839
                     /locus_tag="VF240_08510"
     tRNA            13766..13839
                     /locus_tag="VF240_08510"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13800..13802,aa:Ile,seq:cat)
     gene            13973..14044
                     /locus_tag="VF240_08515"
     tRNA            13973..14044
                     /locus_tag="VF240_08515"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14006..14008,aa:Ala,seq:tgc)
     gene            14049..14128
                     /locus_tag="VF240_08520"
     tRNA            14049..14128
                     /locus_tag="VF240_08520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14086..14088,aa:Thr,seq:cgt)
     gene            14139..14211
                     /locus_tag="VF240_08525"
     tRNA            14139..14211
                     /locus_tag="VF240_08525"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14170..14172,aa:Met,seq:cat)
BASE COUNT         2566 a         4525 c         4416 g         2763 t
ORIGIN      
        1 cgcagctcct cgtcctcgac cttccgcccg gcggtctcca tcgcgccgag gagcgccgac
       61 tccgagtagc gcggcggcgc cttcgtctgc ttggcgagcg cctcggcgtg cgtcacctcg
      121 gccgagtcct tcgccttgag cggcggcagc tccgcgtccg tctcctcctc gtcgtcgccc
      181 gacttcttca agtccttcgc cggcggatcg acctcgcgcc agccggccac aagcaccacg
      241 gcgccgcgcg tgatgaaacg ttcgccctcg gcttcggtga cgacggtggt gcgctcgatc
      301 tcggcgtcgg ggtagaaggc ggcgaggaag cggcgagcga cgaggtcgta gatgcgcttc
      361 tcgtcgggcg acagcgccga cggctcaatc ctctgcttcg tcgggatgac ggcgtggtgg
      421 tcggtcacct tcttgtcgtc catgtgccga ctggtgaggg taattttatc gcgcgccaga
      481 atctcctcga tgaacttcac gtaggggccg acgcgcgccg cctcgacgtg agcgcgcagc
      541 tccttcccca cgtccgcgga gagatggcgc gaggaggtgc gcgggtaggt gaggaacttc
      601 ttctcgtaga gcgtctgagc gagttcgagc gtgcgcgttg ccgtaaatcc gtagcgcacg
      661 ccggccgcgc gctggagcgt cgtcaggtca tagagcagcg gcggccgctc ggtcgctttt
      721 ttcttctcaa ccttcgcgac tgcgccggtc ttccccgaga ccttcgcgac gattgcgtcg
      781 gcgtcttcct tcttgtcgaa gcgcgtgccg tctttgccga accagaggcc gcggtacgcg
      841 cccgaaggcg cgcggaagtc ggcgacgacc tcgaagtagt cagtggggac gaattcgctg
      901 atctcgcggt cgcgctcgac gatgagggcg agcgtgggcg tctgcacgcg gccgagcgag
      961 tagacgcctt cgtggccggc gcggcgggcg cggatggtct gcgcgcgcgt ggcgttgatg
     1021 cccacgagcc agtcggcctg ctggcgcgcg tgcgccgatt cgcgcaagcc tttgtactcg
     1081 gaggcccctt tcagtcggcg gaagccgtcg aggatggcct cgcgcgtcaa ggacgaaatc
     1141 cacaaacgtt cgaccggctt gcggcaaccg gcgagcgtgt agacgaggtc gaagatcagc
     1201 tcgccctcgc gccccgcgtc ggccgcgttg accactgcgg tcacgtcctc gcgccccagc
     1261 agcgacttga tgaccttgaa ctggtcggcc gtccgttgcg acgggtgata cttgaatttt
     1321 tccgggatga agggcaggtc ttccagacgc cagcgttcga gcttcgggtc gtaggagtcg
     1381 gggtcgtcga gttcgacgag gtggccgacg caccatgtga cgatgtcggc ctgtccctcg
     1441 atgaagccgt cgccccgacg cgtcgcgccg agcgccgcgg ccacgtcgcg ccccatcgaa
     1501 ggcttctcgg tgatgacgag tctcatcgtg ccaagacttt aacgcgaccc tgagcgcacg
     1561 acaaaacggc ggcgcttgat ttttatgtaa gggcagggca agtccgcggc cggcataacg
     1621 atggaagcaa ttaacatgga tggacaggat gagcaggaga aagaaaactg attactgatt
     1681 tcagatttgt gatttcaaat ttcagatttt caaatctgtt tatcctgcct gtcctgtcca
     1741 tccatgttaa ttaattttga ggcggcgcgg ggcggcgtgg gccgccgggc cgagtttgat
     1801 ttgatggctg cttcgtaact tcagaaggcc cggcccacgc ggcgcagacc tttctgcgcg
     1861 acctgataag acttctcaag cgtccgccgg gtgacggagg ccgtcgcgcc ggcgacgtgc
     1921 cgggcgcggt gcccgccgcc gcccgcgccg ccgctgacag tcttctcgta gtagtcggcg
     1981 atgaagtcct tcatctcgaa ctgcattctc gacatccctt gttgctccgc ctctacggcc
     2041 gcgcgcttgt acgtccaccc ttcgcgcgag atgcggtagg cggcgacgac cgtgcccgtg
     2101 cggtcttcac catacttgca gtggacgaag acgggccggt tctcgggcgc gtcgataagg
     2161 gcgagggcgc gctcgatctg ctcgtcgtcc gggcgcgcgt agggcggcag cggcacgttg
     2221 aagtattgca gcccggcggc gcgagcctcc gcttcctcgg cgtgcgagtt ttcgtcggcg
     2281 gcgcggaggt tgacgaccgt cctgacgccg agcgcgggga ggcgacgcac ccccccagcg
     2341 cgcggctgcg cgccgcgata gaggtgcgcg ttcacttgat gaaagttcgg cagttcttca
     2401 tacttcggcg cgtcctgcgc tgtggcggcg acggcggcgg agaacataag cgcgagcgcg
     2461 acggcgctca atttacacgt caccttattc ataaaacaca gtggcccgac cgaagtgtat
     2521 ttcgcctgtg gggagtttcg gtgcggacta tatggtaaaa ggcgctgcgt cacaacccgc
     2581 agccgggcga cagtaccgcg cgcgggggag gcaagggatg agggatgagg gatgaatgag
     2641 gacagtcggc agtcaaagcc ggtcgcgcgt cctgactgcc gactgctgac tgttactgct
     2701 tcctcccctt catccctcat ccctcatcct tcatccttcc gccgcacccg ctcgctaccg
     2761 ctcgcggtac tgttataagg aggagttaat tgaccacccc gcagacgaat gcacaccggg
     2821 ccgagttcgg cgtcatcgga ctcgccgtga tgggatcgaa tctcgcactc aacatcgagg
     2881 agcacggctt ccccgtcgcc gtctggaatc tggagaccga gcgcgttgac gagtttctgg
     2941 gcggcgcggg cgcaggccga cgcttcacgg ggacgaagac gttcgagcag ctcgtcgccg
     3001 ccctcgaacg cccccgccgc atcctgatga tgatcaaagc cggcgccccc gtggacatga
     3061 ccatcgagcg acttcgcccg ctgctcgacc ccggcgacat cctcatcgac ggcggcaact
     3121 cgtggttcaa ggatacgcag cggcgtgagg ccgcgtgccg cgaggcgggt ctgaacttcg
     3181 tcggcatggg ggtgtcgggc ggcgaggagg gcgcgcggcg cgggccgtcg ctcatgccgg
     3241 gcgggacggc cgaagcctac gagcgactgc gtcccgtcct cgaagccatc gccgcgaaga
     3301 ccgactcggg cgcgtgcgtc acgcacgtcg ggccggacgg ctcaggccac ttcgtcaaga
     3361 tggttcacaa cggcatcgag tacggcgaca tgcagctcat cgccgaagcc tacgacattc
     3421 tccgcaaatg cctcggcctc gaagccgcgg agcttgccga catcttcgcc gagtggaacc
     3481 gcggcccgct cgaatccttc ctcgtcgaaa tcacgtcaca gattttcgcg gtgcgcgatg
     3541 aggagaccgg acgagcgctc gtcgatctcg tctcggacaa ggccgggcag aaaggcacgg
     3601 gcaagtggac ggcgcaggtg gcgctcgacc tcggcgtgcc cgttccgacc atcgccgccg
     3661 ccatcgacgc gcgcgtcctc tccagcatga aagatgagcg cgtgcgcgcg tccgccctta
     3721 tcgaaggccc gcgcgccggc gcgccggacg gcgtccgtgc ggagttcgtc gccgcagtcc
     3781 acgacgcgct ctacgcctcg aaggtctgcg cctacgcgca gggcatgagc ctcatccggg
     3841 ccgcctcggc cgagtataaa tgggaaacga acgcgcgcga gctggcgcgc atctggaagg
     3901 gcggctgcat catccgggcg cgcttcctcg acaaagtcat gcgcgcctac gagcgcgcgc
     3961 ccggcctcgc caacctgctc ctcgacgaag atatcggccg gagtctgcgc gagtcgcaag
     4021 cggcgtggcg gcggacggtc gcggcggccg tgcgagcggg cgtcccggtc cccgccacgt
     4081 ccgcttccct ggcctacttc gacagctacc gcgcggccga cctgccccag aatctcacgc
     4141 aggcccagcg cgacttcttc ggcgcacaca cctacgagcg cgtcgataag ccggacgcgg
     4201 gtttcataca caccgactgg gcggggctgt ccgggccgac ggattaactc tacaaggtgg
     4261 tgtcggccag attcgacttg tacttggtgt tcgtcttccg gtcgttcaac tcaatgtaga
     4321 cccagctcgg cgggacaaca cccggctcaa gggagaacca aaccttgttg agatcgtcgg
     4381 gttttccgaa ggcgcaaaat ccgttgagcc gctttccgct ctgttcgtag acatccaccc
     4441 aggtgcgtga ggctttcgtg tttagtccgc agggcggcag ctccggcgcg gcggcgaaaa
     4501 aatcagtcgg ataactcgcg aagttatcga ccacgtaccg atacctgagc cattgcttgc
     4561 cgttggcctc gtattgctcc tgcccgatga acacgagcac ggggttgggc agcggccgcg
     4621 gactctgcgc ggtcgaaacg ctgtccgtcg cgagggccat caaaaccaga ctgacaaaaa
     4681 acaacgcggc tttcttccag acgcttaatc tcatcgtggt tgccttcctt ccgtggcgaa
     4741 gcggagacgg ccactcgcct cgccggtgaa caaagtttcc ctctggatag ttaagcaaaa
     4801 ccggattgag taaccagcgc gggtactgag gccccgcgca ttgtaacgcc gaggccaaac
     4861 ttgaattgca cgatgtaacg cggcctgact cgcggccggc tcgaacgacc tgttagaccg
     4921 ccttcgactc cgtgaacgtg aaggtgtgaa gatcagccgc gccgcgtcaa atcattttcg
     4981 gaaggctctc catgaattcg ccgattccca cgtagtaagg attttcgcgc aacatgttgc
     5041 ggcaaaacgt gagggggtgc gtctcgtaag cgccgtgaac gatgacgttg cccgacaggt
     5101 tgttcacgtc atacatacac acgatgacgc tcgggaaggc tttcgccgcg cccgtgacct
     5161 tggcctcgaa cgcaagtatg tccctctcgc tcggccagtt cgcccgcccc cagccgatgt
     5221 tgcccaggag gcgaatgagt ttcgcgcccc cttccaacgc gcgctcgaac gaagccccga
     5281 tgttcgccat gatcaggtcg ccgtcagatt ctccgccgag gagcgtgagc ctgcccgcgg
     5341 cttccagcgc gctcacgtta aatccgtgcc cgatcaaaac ctggcatact ttttcgttgg
     5401 cctcttcaaa gccgaagatc acgcaatgat cctcgccgcg caggccgatt tcaagaaact
     5461 tcaccccctc ggcgaaatct ttttcgttct cccagaaata cgcgacgtga tcccccggtg
     5521 aaagtttctc gcccctgata cccaattcta tcttcttttt gttcataacc gacagcccct
     5581 ttgatagttg aggagggaag ttactatagc agtgtgtcga atacaacgcg ggcggatgga
     5641 cgcctgattt gagtcgccgc gcacggacag gttctcgttg agagtgacga ttactcgcgt
     5701 tgggctggaa tgacctgtca actgccgggt ggaagataag tcactccgcc ggcggccagc
     5761 aaaacagctt gtccgtggaa gacaccgcct cgatggccgt ctccgcgtcg aacaacttcc
     5821 gccggatgac ctcgtcccac aaagcggcgc cgccgcccgt ctccgtgggg atcagataac
     5881 tctccggcct ttcgtccgtg aaaatgtaga cctcgtcgtg ttcatctcaa gttatcttca
     5941 ctccgcggag ccgccgttca gtagccgcac gctctggccg acggcgaggc ggcccgtgcg
     6001 gatgacgcgt gcgtagaggc gcgactcgcc ggggtgtgtc ttctggctga tgcgggcgaa
     6061 gtcgccgccg gcgaaggatt ttttgatggt gccgcagggg gtggtgtagc tgctgatctc
     6121 gatgaccact tcgtcgccga gggcgaggcg ggagccgggc gcgagactcg gccagtcgag
     6181 accttcgacc gtcacgttct cgccgaccga gccggggaag atggggtgcc cttccgactg
     6241 tagccgctgg atgagttcga gcgggaagag acagagggcg cgctcggggc cgccgtggat
     6301 ttttgggtag gccaccttgt cgcccgtgag gcccgtctcc gtgagcacgg cgtcgggcac
     6361 gggggtcttc ggcacgccgc cctgcgaaca gttgagctga tgaattttgc cttccatgat
     6421 tacacagtcg tcagttggca gttgtcagtt gtttccgtcg ccagacttga accttcatgt
     6481 gtgtgcagct agttgcccga cacgaacaac tgaccactga ccactgacta ttgactacta
     6541 ccgtttctta cgattgctcg tggcggcgcc ggcctcccgg tcggtcaggt tcgaggcgtc
     6601 cttcgcctgt tgcggcgcga tggccttctc cttgcgcgtg ttgtcggcct gcctcgcggc
     6661 ctgattcttc ttgtccttgt tgcgtccatc cttcttcttc cgatccttgt ccgacatcaa
     6721 tactccctcc tgccttctcg tttgattcgt agaacgactg ctcagagctg tgaggccacc
     6781 gctggccagc acgcctgtcg cgcaagtgtg ctttcggttt ccagtgacgc ccggattaaa
     6841 tcgacgaggt cggcgaaact ctcgggcgca tagagtggcg gggcgtcggc cattccctgt
     6901 cgcgtcgcaa gctcggcgat ctcgcggcgc gtgagttcgt cggcgcatgc tccggcgagg
     6961 ccgggcaggt agagcgcgtc ggctttggcg agcgcgcggc gggccgtggg ttttattttg
     7021 gcggcggggc cggcaaccat cacgaagaag tctacctcga cgaactgcga gaagctgttg
     7081 ccctcgatga tgacgcccgc cgaggcgacg cgcccgagtg cttccttgat gccgcgctcg
     7141 acctgccggt cggtggcgac gacccagtga acgtccgacg cgcccgcctc ccagtagcgg
     7201 cccgtatcct tgcccttagt gtcagtccgg gcgcgttccg agagcaccag cggctcgtcg
     7261 ctgaggaggt ggctgacgca gcagacgtgt gggtccttgc cgcaggagcg atagtggccg
     7321 cgcgtcacct tgatcgcctc ccagccgggg aaggcgcgca aaagttcgca cacgagcgtc
     7381 gtcttgcccg tgttcgacgc gaacccgccg acgccgactt ttttcagagt catgccgaga
     7441 actcacccgc cgcgcagaat cagtttacaa tgctactacc cggcgacgat ttgtcaaagc
     7501 agttcaaact gagcgcggag gacgcccgcg ccgcgaccga aaagtttatg cgaccgaacg
     7561 aagacctcat cgaagagatc gcccgcgcgc tcgaacgttg cagcgacgcg gcgacgcgcg
     7621 gggagtcgtc cgccacagtc gcccgcgagt gggcggcggc cggcttcgac tccgccgagg
     7681 atgttgagga ctggctcggc gcgcgctgct tcagtgcggc cggcgctaaa gccctcgacg
     7741 atgcggggct gacgcccgag caggccgcca tccgcacacg cgaaggcgcg accgactacg
     7801 aagacaccat cggcttcaaa gtttccaacg gcgacctgag caccgacgcc gcgcgacgca
     7861 tcgccaccaa cagcttctgg aacacttaga acgcgtcgca agactgtagg ggcaaggcaa
     7921 gtccgcggcc cgcggttgcc aaagcaatta acatggatgg acaagatgga taggagatga
     7981 agatttgaaa tctggaattt gaaatctcaa atcagcgctt tcttcatcct gactatcctg
     8041 cccatccatg ttaattgttc tgaaagttga gtgggcaggg acgagccctg cccctacagt
     8101 cgtgaccaac gttgagaggt tttgcaacgg actcttagca cgaagatggc gaaactccac
     8161 gaactccgct tcgataacac ctacgcgcgc ctgcccccgg ccttctactc gaagctcgcg
     8221 ccgacgccgc tcgctgagcc gcatcttgtc agcttcaacc cggcggcggc cgagttgatc
     8281 ggccttgatg cgtccgaggc cgagcgcccg gagtttgtcg aagctttcgg cggcaaaagt
     8341 ctcccgcgcg gcgcggagcc gctcgcgatg ctctacgcgg gccaccagtt cggcgcctac
     8401 gtcccgcagc tcggcgacgg ccgcgccatt ctcctcggag aggtgcgcgg ccccgccgga
     8461 gacaagtggg acctgcacct gaaaggctcc ggccccacgc cctactctcg cggcttcgac
     8521 ggccgcgccg tgctgcgctc gaccgtccgc gaatacctct gcggcgaggc gttgcacggc
     8581 ttgggcgtcc ctacgacgcg cgccctctgc atcgtcggaa gcagcgagcc ggtggtgcgc
     8641 gagcgcgtcg agagcgccgc ggcgctcgtg cgtctctcac cctcacacgt ccgcttcggc
     8701 accttcgagg tcttccaccg gcgcgctcag cacgcgcacg tccggcagct tgctgattat
     8761 gtcatcggcc aacacttccc ggagttgtcg ggcctgcctg acgggcacgc gcggttcttc
     8821 tccgaggtcg ccacacgcac cgcccgcctc atcgccatgt ggcaggccca cggcttcgcg
     8881 cacggcgtga tgaatacgga caacatgtcc atcctcggcc tcaccatcga ctacggcccc
     8941 ttcggcttca tcgacgactt cgaccccggc tacgtctgta accactccga ctacgccggc
     9001 cgctacgcct tcgaccaaca gcccgccgtc ggcctctgga acctcacgcg actggccgaa
     9061 gccctctcca cgctgctcgc gcccgaacag atcgagtcgg ccctccaact ctacccggcc
     9121 gccttcgcca atgcctacgc cgacttgatg cgacgaaaac tcggcctgcg tgaagagcgc
     9181 cccgccgacg cgcagctcgt tgcccgcctt ctcgaactcc tgcaacgggg ccacgtcgat
     9241 tacccgcgat tcttccgcgc cctcggcgat tttcaagagg ctcccggcgc gacaaacgaa
     9301 acgctacgcg tcatgttcga cagccccgcg gacttcgacg cctgggccgc cgactaccgc
     9361 gctcgactgt cggaagaacg aagcgagcca gcggcgcgca aagccttgat ggattccgtc
     9421 aacccgaagt acgtgctccg caactacctc gcccagaacg ccatcacccg cgccgagggc
     9481 cgcgactatt cggagattca aagactcctc gaactcctca gcgacccctt cgccgagcac
     9541 cccggcatgg aagactacgc cgcgccgcct cccgcctggg gcagagggct ggttcttagc
     9601 tgctcctctt agctgccggc cccgagtcac ctcctgaact cgtcagcacc ctccgtcgcg
     9661 gcgaacgcct tattgtcagc ttcaaatcgc ccccttaaaa aagtacttga caatgcaaag
     9721 tgcattgtcg tatatacact ccacgcgccc ggtgtgaagc cgggcagcgg tggagacgaa
     9781 agacttcgat gttaaagaac gcgacaagaa taaaaggctc cgacgccgcc acgacgaccg
     9841 gccaggtcgc gtggccgttg ctcccgcgcg agggcgcggg gcgcggtagg ggcttcgcct
     9901 gtctgagttc ggcgggcatt aattgccccg gcgttcgact gggcgggcgg caagccggaa
     9961 ggcccaaagg catgcagcct tctgatcgtc acaatttgaa tatttgatta cgcgggagcg
    10021 ggcaacctcc cgctcctgtt acaaccgaaa gttcaggtaa ctgtgagcgg gtaaatgctt
    10081 ctctcgggaa gtgtttaccc gctctctttt tttgcggctg cgggagccgg agccgcgggg
    10141 ggcttgcgag accccacagt tgtttgacaa tcgaaatacc ttgtggctgt gaaggcggcg
    10201 accgactggg gccgccccgt gcgcggactc tccgagaacg tcgccgcctt cacagatttt
    10261 ttctcttatg cgctcgtccg ccgagcgcga gccgtcgagc atcagacgcc caaacggcag
    10321 ggcagcggac tgtaaccccg tggcttaaca aagcgtggag gttcgactcc tccctgatgc
    10381 atgacggaag gctaggctaa ctggtaagcc agcagtctcg aaaactgccg tgcgcccgac
    10441 gagggcgttt gtgagttcga atctcacgcc ttccttccta cttcgtggag acgtggccga
    10501 atggtgatgg cagcggcgtg ctaagccgcc gggcgattgt cccgtgtgag ttcgagtctc
    10561 accgtctccg atttggaccc gtagctcagt cggaatcaga gcgcaggttt gcggagcctg
    10621 aggtcgcacg ttcgagtcgt gccgggttca ttttttcaag tcgcgaaaga gtgtttacgg
    10681 gatggtgaaa gcggcatcac gccgggcttt gaactcggag ttgagggttc gagtccttct
    10741 cccgtagcca gactttttcc cgccgacgta gcccaacggg cagaggcgtc agtcttagga
    10801 actgattagt aagagttcga atctcttcgt cggcacttat acgctggagt ggcggaacag
    10861 gcatacgcgc ggcgccgaga atgccgtgag cacatcggct cagtgtgggt tcaaatccca
    10921 cctccagcac caacttcggc gggcgcgtgt ggcccaattg gcagaggcgg ttctcttaaa
    10981 aagaacttcg tgcgggttcg aatcccgcca cgcgcacttt ggaaacgcac gcgcgcgcgt
    11041 agcccaacgg gtagaggcgg cggactcaaa atccgcccgt tgaaagttcg aatctttccg
    11101 cgcgcatctt tcaacctctg ggggcgtagc ataacggcca atgcagcggg ttccaacctc
    11161 gtgatccttt caatgggaca tatgcctgtt cgaatcgggc cgcccctgcc aacctctttc
    11221 ccacggggat gtatgcaaat ggacaacgcg gcttgacctt caatcaagcg ccctccgagg
    11281 ctttgcgggt tcgagtcccg ccatccctac ctttagtcca aagttcaaag ttcaaagttc
    11341 aaagcgcgtc cggcggctgt tgactttaaa ccttgaactt tgaactttga acttcagttc
    11401 gttgtggtgg cgttggtcta tgaggtaaga cgtgcgcctg tgaagcgcag gaagcgagtt
    11461 cgagtctcgc acgtcaccct ccacacgcgc gcgggtggaa agtttcccgc cccgcgcaaa
    11521 cttcgggacc gtggccgaga ggatcaggca cttgattgtc gatcaagacc acgcgggttc
    11581 gattcccgtc ggttccgttt tcagcctccg tagcttaaac gcgcagagcg gcgatctctt
    11641 aaatcgcaag gttcgagttc aactctcgac gggggcgcca actcgttcat cggagcgtag
    11701 cctagcggct tcaggcactt gacctgggat caagacgacg aaggttcgaa tccttccgct
    11761 ccgaccaatc attccctctc acacgcgaac gtagctcaac cggaaacaag agtgccggcg
    11821 tgccatgccg gagacgcggg ttcgactccc gccgttcgct tccgtttcaa cgggcggtta
    11881 gcttaacgga acaaaaagcg ggccgcttcg aacggctcca gatgcgggtt cgaatcctgc
    11941 accgcccttc cgctacttca cttccaacat tcaggcacgc gtagccaagc tggttaaggc
    12001 gacggtctgc aaaaccgtta atcgccggtt cgattccggc cgcgtgcttt cgccggagtc
    12061 tggggcacac ccaaacgggg aggcgaaacc gccgtcccgt gtgtcggcag gctcaaaccg
    12121 tcaaccgtca aacaaaggag gtgcgcggag attgtgagac gtgccgaaag cttcgcggcg
    12181 tcgagccgac ggccgtcgcg cgctgacgtg cggcggtggc ggcgaggcgc gtgaggtttt
    12241 ttgtgaatga ttggatcacc tgaattgaaa gcacaagacg tgtgcggccg tctacgttcg
    12301 gccgcacacg caacttttgc gagggacgcc gcgcctgcgg ccgtcctcac caccctcgca
    12361 aaaccctttt agcgcctccc gtgcctcccg cgtgaaacta cttgtctgcg cggcgaagaa
    12421 gtttcacgcg ggcggcgcgg gaggcgcaga gtagaagggt gatgtggcgg agtcttaaac
    12481 ctgaacgatc aaaatctatg aaaggaggtg ccgtgatgtc tttgaatggc cgagttttgc
    12541 ttctgaactt ttcctacgag ccgctcggca ccgtgggtgt ggcgcgggcg atgtgcctcg
    12601 tgatgagagg ggccgttttc gtcgaggagt acgagccggg ccgcgtgctc cgctcgcctt
    12661 cgcgtgagtt tccggtgccg agtgtcgtcc gcctgcgcgc ctacgtcaac gtcagacgcc
    12721 gaaggcagac ggccggaatg cgacggctgc gcatcttcgt gcgcgaccgt ttccgctgcc
    12781 agtactgcgg ccgcaagcag gaggcgtcga aactgtcgct cgaccacatc ctgccgcgct
    12841 cgcgtgcggg ccgctccggg ccggagaatc tggtgacggc gtgcgtccgc tgcaacacgc
    12901 gcaagggcaa ccgcacgccg gaagaggcgc gcatgccgct gctcaccgcg gagcgcgcgc
    12961 tcacggtcgg cctcgaccgc gtgatgctct gccactacgc ggagtcgcgc ccggagtggc
    13021 gcaagtacat gttcatggcc gaggcggagg ccgacgagca tgagctgcgc cgggcatgaa
    13081 cggcgcgcgg ggagtttccg gtaaccggct ccccgcgcgc cggcttccgg tttctgatgt
    13141 tttcccaacg cggctgggta gctgagacgg attagcgccg gtttgaaaca ccgaacaggt
    13201 tggttcgatt ccaaccccag ccacttcttc ccccgacttt ctcgatgtgg tctagctcaa
    13261 ctggtagagc gctgctttgt taaagcagtc ggtgtgggtt cgactcccac ggccacagtt
    13321 cattcacacg gccggttagc tcaacccggt agagcggcag tcctacaaac tgcgggttga
    13381 aggttcgact ccttcaccgg ccacttgggg agtgatgagt gataagtgat aagtgataag
    13441 aaagacaaag cccgtctgct ctttcttatc acttatcact tatcactcat cactcatcgg
    13501 ggcgtagctc aaacgcggct gaaacgtgaa cagcgggagc gtttagcgca gaggccgcgg
    13561 acgacctcgc gccccggcca aacttttgcg gcgatgtagc tctaattggc agagcagttt
    13621 tctcgtaaaa aacagcgtgt gggttcgact cccaccatcg cctttcgact attttgggtc
    13681 cgtagctcag tcggaatcag agcgtctgcc ttctaagcag agggtcgcag gttcgagtcc
    13741 tgccgggcct gttggttgaa actgatgcgg ggaagcacag gaggtgtgtg cagcggtctc
    13801 ataaaccgaa ggtcgcgggt tcaagtcccg cccccgcaat tggaataatc gtgaatcgtc
    13861 aatcgtcaat cgtaaatcga taaggttgtc gcccaacgat ctgagcggcg tcactcaagg
    13921 tggcccgact caaccacgat tcacgattga cgattgacga ttcacgattt tggggccggt
    13981 gatgtaacgg gaagcatggg agtcctgcaa actcttcgcg agagttcgaa tctctcccgg
    14041 tccatttttg cggcgtagct caaacggtta acagagcgcg cgtctcgtaa acgcgaaggt
    14101 gcgggttcga gtcccgccgc cgcagccagc gatccacagc ggggaggagc ctcggaggct
    14161 cggcggtctc ataagccgcg acacatagca ggttcgactc ctgcccccgc gcccctcatt
    14221 gacccggagc acgagcggca gtgtggcagc cttttaagct gtgaggtgcg
//
LOCUS       DASVES010000214         6791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1167806, whole genome shotgun sequence.
ACCESSION   DASVES010000214 DASVES010000000
VERSION     DASVES010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167806"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VF240_08530"
     CDS             <1..640
                     /locus_tag="VF240_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX7175288.1"
                     /translation="IISIVGYTNAGKSTLLNTLTSSEVLAEKRMFATLDPTSRRLRLP
                     REQEVIINDTVGFIRDLPPDLLAAFRATLEEIDQSDLLVHLVDAANPRWQQQLESVEN
                     ILGELGHTQIPRIVAFNKADLLTPDDVDALLRQACLAGARECVALSAIDPKSIRPLLE
                     RAGEILARDLTEGAHDESNSEPDDAGDALVESQASAEDAEPEEFETATQSSK"
     gene            637..3087
                     /locus_tag="VF240_08535"
     CDS             637..3087
                     /locus_tag="VF240_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX7175289.1"
                     /translation="MSRRRQGRRPRLSTPLSEARMAVVRTLSRPFNRLPPLARFLVGF
                     LALTLLTTLLLAWTRSQMPADVYQEGDVVRSDVIAPADITAVDEEETKRRRAQAAESA
                     PPVWSYDPTRAESAAQSFRNSWETLKRQYERRRGGDSNSNGAAGNTSDERADLLWPGA
                     GNPAAVARALVAHRFDAGAFEQLTNMIRESGEGYVYDDAEAVHLRKELRVVDSLSGTQ
                     TDVSQNRAFSLSLARGGLRGRASQLSGWTPEEREVVASAVLPLLGASITFDRTATEAA
                     RLAAAEAVAPVVVSLKRNQIVAREHDSATPQTLAQLAAIRSYGRTTRRPHHFVGIFLL
                     VAALYGAAWRFIEYRSSTTTLSLSKAKAFSLVALSLLVQLLLMRVGFLLAQSISGQSM
                     TPPLNDPVVWSFAIPFAAGALLIAMLVDTQLALIAGVLAAIFAGLLAPNGILMACYAL
                     VSSSAAIYGMGRYRERQSVTLAGLIVGLINALIAVAILLTAQQPLTLNTALLAMGCGL
                     LGGVLTTIFTAGGLPINESMFGILTDVKLLELSNADLPVLGQLALRAPGTNQHSHAVG
                     QLAEDACRAIGANPLLARIGALYHDIGKLAAPDHFIENQSGDNPHDRLRPQQSARIIT
                     SHVTYGLKLAREIGLPTQISDFIPQHHGTRTLHFFLRKAQAQAAEGETVEEAEFRYPG
                     PKPQFKEAAVMMLADSCEAAARSLARPDPENIRAIVTKIFDAILSDGQLDECDLTLRE
                     VTSIRETIIASLTAIYHARIDYPGFNPPSLDPTRPPLPPADLDSEERGVGYRRPSEVP
                     ISKGGEVEDEALTGEGKR"
     gene            3268..3738
                     /locus_tag="VF240_08540"
     CDS             3268..3738
                     /locus_tag="VF240_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175290.1"
                     /translation="MALALSGKSVQVAERGGEKVEVKTRQDWQEKHIEGVKGIVSGAI
                     LLAVSLLIGVALALFVPGDAPWILIWMVFFGWLAVWGGIEMASGVGGVIEAKGRLRMM
                     KQAGTEPAIDASPQQLSSSGEPVTIEHSSSASRSTPPLSVTEGTTRHLDEFVEK"
     gene            3809..4762
                     /locus_tag="VF240_08545"
     CDS             3809..4762
                     /locus_tag="VF240_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7175291.1"
                     /translation="MPSWQARCLNGVVRVLIRRRTWGNDERAVARRARRLFGTPAPFH
                     LLRTRGLRLEQVDDAGVRGEWVTCENPERGVILYVHGGGFVSCSAATHRPITAALARL
                     ARQRVFSINYRLAPEHRFPAGLDDAFAAYRWLLGRALSASDISIAGDSAGGNLVLGVL
                     LRARDAGLPLPASAVCFSPWTDLAGTGDSVRLNDGRCAMFRPESIDEFAASYLDGMSS
                     TRDPRASPVYADLGGLPPLLLQVGSTELLLDDARRVHHKILKTGGSSRLEIFDDVFHC
                     WQMLDGFVPEARTALRQAADFIRSHAPAAEVVPSGNGNPNI"
     gene            4801..5187
                     /locus_tag="VF240_08550"
     CDS             4801..5187
                     /locus_tag="VF240_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX7175292.1"
                     /translation="MEIKNILTQNAPAPGGHYSQAVVHNGLVYVAGQLAIDPRTGERK
                     LGSIEEQTEQVLRNVAAILEAAGSDLSRVLKMTVYVADIELWAAVNTVYARVMGEHRP
                     ARAIVPTKELHYGFLVEIDAIAATNP"
     gene            5253..6257
                     /locus_tag="VF240_08555"
     CDS             5253..6257
                     /locus_tag="VF240_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175293.1"
                     /translation="MRLKLALALAFLCCAQSAAAQARPRVREADRVRLAEAFRLGETL
                     GDRIWTGWSKAPFAVLLVTPEGEFLVRHPKPPQDFAPVGHDPLLRGEVFFREPKLNPA
                     LLATFPVGGVPTIVIGQAENTASKTSTPWVLTVLHEHFHQLQYSQPNYYDAVGGLNLS
                     RGDQTGMWMLNFAFPYREAEVQSRFESLSKLLAEALQARGKKDFSTRLARYLEARRVF
                     ASALAPDDYRYFSFQVWQEGVSRYTEYRIAELAAKSYKPSKGFRELKDYTPFAEVADR
                     SHQGVLSELTTLRLESYGRVAFYPLGAGEGLLLDRVNRRWRDRYFADKFYLEKYFGAR
                     "
     gene            complement(6467..>6791)
                     /locus_tag="VF240_08560"
     CDS             complement(6467..>6791)
                     /locus_tag="VF240_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7175294.1"
                     /translation="YPYVSGHLLIVPLEHAPDLDAVSKQATDELMDLSKRALVLLRDA
                     YRPHGFNLGMNLGQAAGAGVADHVHLHVMPRWSGDANFMTTVGETRVLPEDLRTTYER
                     LKGKF"
BASE COUNT         1158 a         2375 c         2108 g         1150 t
ORIGIN      
        1 catcatctcc atcgtcggct acaccaacgc gggcaagtcc actctgctga acacgctcac
       61 ctcttcggaa gttctcgccg agaagcgcat gttcgccacg ctcgacccga cctcgcgccg
      121 cctgcgcctg ccgcgcgagc aggaggtgat tatcaacgac acggtcggct tcatccgcga
      181 cctgccgccc gatctcctcg ccgccttccg cgccacgctc gaagagatcg accagagcga
      241 cctcctcgtc cacctcgttg acgccgcgaa cccgcgctgg caacagcagc ttgaatcggt
      301 ggaaaacatc ctcggcgaac tcggtcacac acagattccc cgcatcgtcg ccttcaacaa
      361 ggccgacctg ctcacgcccg acgacgtgga cgcgctcctg cgacaggcgt gtcttgcggg
      421 cgcgcgcgag tgcgtcgccc tctcggccat cgacccgaag agcattcgtc cgctccttga
      481 gcgcgcgggc gaaatcctcg cgcgcgacct gacggaaggg gcgcacgacg agtctaatag
      541 cgaacctgac gacgcgggag atgcgttagt cgaaagccaa gccagcgcgg aggacgcgga
      601 gccggaagag tttgagacgg cgacgcagtc aagcaaatga gcaggcgacg acagggacgg
      661 cggccgagat tatccacgcc tctctccgag gcgcgcatgg ccgtcgtccg cactctctcc
      721 cggcccttta atcgtctccc gcccctcgcg cgcttcctcg tcggcttcct cgctctcacc
      781 ttactgacca cgctgctcct cgcctggacg cgttcgcaga tgcccgcgga cgtttatcag
      841 gagggcgatg tcgtgcgctc cgacgtaatc gcgcccgccg acatcaccgc cgtggacgag
      901 gaggagacca agcgacgccg cgcacaggcc gcggagtcgg ccccgcccgt ctggagctac
      961 gacccgacgc gcgccgagtc ggccgcgcaa tccttccgca actcatggga gacgctgaag
     1021 cgacagtacg agcgacgccg cggcggcgac tccaactcga acggcgcggc cggcaacacg
     1081 agcgacgagc gcgccgacct tctctggccc ggcgcgggca accccgcggc agtggcccgc
     1141 gccctcgtcg cccatcgctt cgacgccggg gccttcgagc agctcacgaa catgatccgt
     1201 gagtcgggtg agggctacgt ctacgacgat gcggaggccg tccacctgcg taaggagttg
     1261 cgcgtcgtgg actcgctttc gggcacgcag acggacgtgt cgcagaaccg cgcgttctcg
     1321 ctctcgctcg cgcgcggcgg attgcgcggg cgcgcctcgc agctctcggg ttggacgccg
     1381 gaggagcgcg aggtcgtcgc ctcggcggtg ctgccgctgc tcggcgcgtc gatcaccttc
     1441 gaccggacgg cgacggaggc ggcacgactc gcggcggccg aggccgtcgc gccggtcgtc
     1501 gtctcgctca agcgcaacca gatcgtcgcg cgcgagcacg acagcgccac gccgcagacg
     1561 ctcgcgcagc tcgccgccat ccgctcttac ggccgcacga cccgccgccc gcaccacttc
     1621 gtcggcatct tcctgctcgt cgccgcgctc tacggcgccg cctggcgctt catcgaatac
     1681 cgtagctcga ccacgacgct gtcgctctcg aaggcgaagg cgttctcgct cgtcgcgctc
     1741 tcgctgctcg tccagttgtt gctgatgcgc gtcggcttcc tcctcgcgca gagcatctcg
     1801 gggcagtcga tgacgccgcc ccttaacgac cccgtcgtgt ggagcttcgc gatccccttc
     1861 gcggcgggcg cgctcctcat cgcgatgctt gtggacacac agctcgcgct catcgcgggc
     1921 gtgctcgcgg ccatcttcgc cggcctgctc gcgcccaacg gcatcctcat ggcctgctac
     1981 gcgctcgtct cctcgtcggc cgccatctac ggcatgggcc gctaccgcga gcggcagtcc
     2041 gtgacgctcg ccggcctcat cgtcggcctg atcaacgcgc tcatcgccgt cgccatcctg
     2101 ctcacggccc agcagcccct gacgctcaat accgcgctcc tcgcgatggg ctgcggcctc
     2161 ctcggcggcg tgctcaccac catcttcacg gccggcggac tccccatcaa cgaatcgatg
     2221 ttcggcatcc tcaccgacgt gaaactgctg gagctgtcga acgccgatct gcccgtgctc
     2281 ggacaactcg ccctgcgcgc gcccggcacc aaccagcact cgcacgccgt cggacaactt
     2341 gccgaagacg cctgccgcgc catcggcgct aaccccttgc tcgcgcgcat cggcgccctc
     2401 taccacgaca tcggcaagct cgccgccccc gaccacttca tcgagaacca gtcgggcgac
     2461 aacccgcacg accgcctgcg cccgcagcag agcgcgcgca tcatcacgag ccacgtcacc
     2521 tacggcctca agctcgcccg cgagatcggc ctccccacgc aaatctccga cttcatcccc
     2581 cagcaccacg gcacgcgcac gctccacttc ttcctccgca aggcgcaggc gcaggccgcc
     2641 gaaggcgaga ctgtggaaga agccgagttt cgttaccccg gccccaagcc gcagttcaag
     2701 gaggcggccg tgatgatgct cgccgactcc tgcgaggccg ccgcgcgctc gctcgcccgc
     2761 cccgaccccg agaacatccg cgccatcgtc accaaaatct tcgacgccat cctctcggac
     2821 ggccagctcg acgagtgcga cctgacgctt cgcgaagtca cgagcatccg cgagaccatc
     2881 atcgcctcgc tcaccgccat ctaccatgcg cgcatcgact accccggctt caacccgccc
     2941 tcgctcgacc ccacacgccc cccgctccct ccagccgacc tcgacagcga ggagcgcggc
     3001 gtcggctacc gtcgcccctc cgaagtcccc atcagcaagg gcggcgaggt cgaagatgag
     3061 gcgctgacgg gcgagggcaa gaggtagagt tttttctgac gattccagaa cccggcgtcg
     3121 tgctgccccg taagaaaatg taggccgggc ggttgctcgt tatctactat caggaaggag
     3181 gcggcagcaa tgtattgtcc gcaatgcgcg acaccacaca cggacggcgc gaagttctgt
     3241 cgctcctgtg ggacggaaat ggaggccgtg gcgctggcgc tgagcggcaa gtctgtgcaa
     3301 gttgctgagc gcggcggaga gaaggtcgag gttaagaccc ggcaggactg gcaggagaag
     3361 cacatcgaag gcgtcaaagg tatcgtcagc ggggcgatcc tgctggcggt gtcgctgcta
     3421 ataggcgtcg ccctggcgtt gttcgtgccg ggagatgctc cgtggatact catctggatg
     3481 gtcttcttcg gatggctggc cgtctggggc ggcatcgaga tggctagcgg cgtcggcggt
     3541 gtgatcgagg cgaaaggcag attgcgcatg atgaaacagg cgggcactga gcctgccatt
     3601 gacgcgtcac ctcagcagtt gtcgtcttcc ggcgagccgg tgacgattga gcattcttcc
     3661 agcgcttcga ggtcaacgcc tccgctgagt gtcaccgagg ggacgacgcg gcatctcgac
     3721 gagtttgtcg agaagtaggg ttgcgcgcat catgccgtcc atgtcgcgcc aacagtcatt
     3781 cgtgtcgagc catcctaaaa ctttcatcat gcccagttgg caagcgcgct gtctgaacgg
     3841 ggtcgtccgg gtgctcatcc gcagacggac gtggggaaat gacgagcggg cggtcgctcg
     3901 ccgcgcgcgt cgcctgttcg gcacgcccgc gccgtttcac ttgcttcgga ctcgtgggct
     3961 gcgcctcgaa caagttgacg atgcgggcgt gcgcggcgag tgggtgacgt gtgagaaccc
     4021 ggagcggggc gtgattctat acgttcacgg tggcggcttc gtgtcctgct cggccgccac
     4081 acaccgcccc atcacggccg ccctcgcgcg cctcgcccgc caacgcgtct tcagcatcaa
     4141 ctatcgcctt gcgcccgaac atcgcttccc ggcggggctg gacgacgcct tcgccgccta
     4201 ccgctggctg ctcgggcgcg ctctgtccgc gagcgatatc tccatcgccg gagattcagc
     4261 cggcggcaac ctcgtgctcg gcgtcctgct gcgggcgcgc gacgcggggc tgccgctgcc
     4321 agcgtccgcc gtgtgcttct cgccgtggac tgatctggcg ggcacgggcg actccgtgcg
     4381 cctcaacgac gggcgttgcg ccatgtttcg cccggagagc attgacgagt ttgctgccag
     4441 ctacctcgac ggcatgtcgt caacgcgcga cccacgcgcg tcgcccgtct acgccgacct
     4501 cggcggatta ccgccgctgc ttctgcaagt cggctcgacg gagttgctgc tcgatgacgc
     4561 acgccgcgtc catcataaaa ttctgaagac cggcgggagc agccggctgg agattttcga
     4621 cgacgtgttc cactgctggc agatgctcga cggcttcgtc cccgaggcgc gcacggcgct
     4681 gcggcaggcc gccgacttca tccgctcaca cgcgccggcg gcagaagttg tgccgtcagg
     4741 caacggcaat ccaaacatct gacaccgggc gcccgaagcc cgcaaggtat ctcgtcaatc
     4801 atggaaatca aaaacatact cacgcaaaac gcccccgcgc cgggaggcca ttattcacag
     4861 gccgtcgtcc acaacggcct cgtctacgtc gccggccagc tcgccatcga cccgcggacg
     4921 ggcgagcgaa agcttggctc aatcgaggag cagacggagc aggtgctccg caacgtggcc
     4981 gcgattctgg aggcggccgg cagcgacctg agccgagtcc tgaagatgac cgtctacgtc
     5041 gcggacatcg aactgtgggc ggcggtcaac accgtctacg cgcgcgtcat gggcgaacac
     5101 cgcccggctc gcgccatcgt cccgaccaaa gaactacact acggcttcct cgtcgagatc
     5161 gacgccatcg ccgcgacgaa cccctgacga acggccggcc cggcgtgacc ccaaaagtgg
     5221 aaccaatcac cttccgcccc tgaatcaaaa acatgaggct caaactcgcc ctcgcgctcg
     5281 ccttcctgtg ctgcgcgcaa agcgcggcgg cgcaggcgag accgcgcgtc cgtgaggccg
     5341 accgcgtccg gctggctgag gctttcaggc ttggcgaaac tctcggcgac cgtatctgga
     5401 cgggatggag caaggcgccc tttgcggtgc tgctcgtcac gccggagggc gagtttcttg
     5461 tacgccatcc caaaccgccg caggacttcg cgcccgtcgg ccacgatccg ctcctgcgcg
     5521 gcgaagtgtt cttccgcgag ccgaagctca acccggcctt gctcgccacc ttccccgtcg
     5581 gcggcgtgcc caccatcgtc atcggtcagg cggaaaatac cgcgtcgaag acctccacgc
     5641 cctgggtgtt gacggtgctc cacgaacact ttcaccagtt gcaatactca cagccaaact
     5701 attacgacgc cgtgggcggc ctgaacctct cgcgcggcga ccagacgggg atgtggatgc
     5761 tcaacttcgc cttcccctac cgggaagccg aagtgcagag ccggttcgag tcactcagca
     5821 aacttctcgc cgaggcgttg caggcgcgcg ggaagaaaga cttctccacc cggctcgcgc
     5881 gctacctcga agcgcgtcgg gtcttcgctt cggcactcgc ccctgacgat tacaggtact
     5941 tctcttttca ggtgtggcag gagggcgtgt cgcgatacac ggagtaccgg attgccgagc
     6001 tggcggcgaa gagttacaag ccgagcaagg gatttcggga gctaaaggat tacacgccct
     6061 tcgcggaagt cgccgaccgc agccatcaag gcgtgctcag tgaattgacc acgctccgtc
     6121 tggagagtta cgggcgtgtg gctttctatc cgctgggcgc gggcgagggg ctgctgctcg
     6181 acagggtcaa caggcgttgg cgggatcgct acttcgccga caagttttat ctcgaaaagt
     6241 atttcggcgc gcgttgaccg cgacactttg caaacctctc ggcgacgcac ttatcagcgg
     6301 gctttatagt cattaggtga gtgaaggcag aagtgattca ccgcggaggc gcagaggacg
     6361 cggaggggcg cggatgcttg aaccgaactc aactctctgc gcttcctccg cgtcctccgc
     6421 gcctccgcgg tgaatctact ttcattccac tcaagtaaag agcgcgctaa aactttccct
     6481 ttagcctctc gtaagtcgtc cgcaggtctt cggggaggac gcgggtttcg ccgacggtgg
     6541 tcatgaagtt ggcgtcgccc gaccagcggg gcatgacgtg gaggtgtacg tgatcggcca
     6601 cgccggctcc ggccgcctgt ccgaggttca ttccgaggtt gaagccatgc gggcgatagg
     6661 cgtcgcgcag caggacgaga gcgcgcttgg agaggtccat tagctcatcc gtggcctgct
     6721 tcgagacggc gtcgaggtcg ggggcgtgtt cgagtgggac gatgaggagg tggccgctga
     6781 cgtaaggata g
//
LOCUS       DASVES010000215        17068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_236629, whole genome shotgun sequence.
ACCESSION   DASVES010000215 DASVES010000000
VERSION     DASVES010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236629"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2817)
                     /gene="tssM"
                     /locus_tag="VF240_08565"
     CDS             complement(<1..2817)
                     /gene="tssM"
                     /locus_tag="VF240_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEX7175295.1"
                     /translation="MSSTGTQVKTALLIAGKIFLVGLLVLLVLFLVPASTPVKILLVA
                     LVLLAWPAWVLLKHFRRSRAADGGGSDAAQNGGKGAKSLPPPTGTYEELTRGTEEVVQ
                     WLRGSKLAGGASADAAYALPWYVVAGPRGSGKTSLLLSSGLDFHVLPSQRASEQRVVR
                     PTEHCDWKVTDSAVWLDTTGRYQTEGPERDEWAALIETVKRHRRTRPVDGFVLAANAA
                     DLLRMNEAQIEQQAKVLRARLDEAMARAGARFPVYLVFTHMDAVEGFGEFFDSFAPPE
                     RAQVWGSTFPLAQGDGAQAQFDAEFEYLYGRLLRRRMVQLGVSAKPEPQLRVFKFPGR
                     FRRTRARLGAFATALFRPNPFSESPLLRGVYFTSSAGAGPTGAKQLQGGEYFTQSLFR
                     DVLLPDRNVVAAAQAARRSPHLRRNIMFGALAALLLFLVGGMVVSFFANKRLIADADA
                     RGRRLTDIRKATSRGRATEAQVADELRAVEDVREVLAELDEHERNSPPVLMRFGLYAG
                     SKLNSSDPASPSILRHIYFEAIEDRFLKPAVAKMEEDLREFGRSGPTGGPATTATTRP
                     TPSPAATPGSSSQAALASATSASTEEDYLGRHYDLLKAYLMLSKPDKVEPTFLAQVLR
                     EYWEEVAPAGTAEVSIRQLEYFSTQAGREDAPHPEIDNSLIDGAQNKLVAYPVVNRVY
                     KRITSEINGAVKFPVKLASIPNAREGGVINNTYSVPGAFTRDGHTELTERLKSSVADE
                     FRKDDWVMRTSLAAGENIDAKKDELANMYYRDYVAHWQRFLQETKIRDFQSKEDAVRS
                     LRLLSGSTSPLESLLQEVSRQTNLSGADGDGVMAWLKGLFTSKTGIGVTPVDKEFRPL
                     VQFVSGKADASPMAEHRAQLKKVADALNANNKPLSELSKAMQAGDDTIGLRPARQAVA
                     DSLEAKGFSSSPASGDAARVLRLPL"
     gene            complement(3182..3892)
                     /gene="icmH"
                     /locus_tag="VF240_08570"
     CDS             complement(3182..3892)
                     /gene="icmH"
                     /locus_tag="VF240_08570"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEX7175296.1"
                     /translation="MSAVAEMPAAAAARRRQRTTNDLINLSAPVLELVLKIRSGAIEP
                     SNDIRGVMDGLLRQLEEGGVQLRCDPRRIRDVKFALVAFVDETVLSPKHNFPLRQQWE
                     GQPLQLVYFEEHMAGMVFFDRLEEMFKDLEANVEVIELYYLCLLLGFKGKYNVYLLEE
                     QLKETIARVAERLRAAGRLKPNALSGHWQAADHPEPPRDPGWPLWAKIGGPALLVFAL
                     LTYLVLYMLLQRELNIVR"
     gene            complement(3985..5331)
                     /gene="tssK"
                     /locus_tag="VF240_08575"
     CDS             complement(3985..5331)
                     /gene="tssK"
                     /locus_tag="VF240_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX7175297.1"
                     /translation="MYRKIVWNEGMLLTPHHFQQWDNYYEELLNSRLASLSPYEWGVL
                     DLQLSQEAIANGFCEVARCRAVLPDGLVVNIPQMDAAPDPRQVGDHFEAGDESLDVHL
                     AVPAKRAGAANFQSNGSERSQFVRYWQDAATVQDETTGQGEQQLAFARPNLRLMFASE
                     LRDGYNSVKIAEVRRTTTGQLTYNANYVPPALSVSSSPWLGQMLRQIIEILIAKSSSM
                     SDQRRQRTTSLADFTTSEIAVFWLLHTVNTSIPVLAHLFRTRLVHPERLYTEMAELCG
                     KLMTFSTERQPQQIVHYQHTDLYATFSQLAAELRGLLETVIPTRSVQIPLESTRESVY
                     VGRVQDERLLDTAEFYLGVSAKVPEGQLIERVPRIVKIASRDVIDGVIGSGLPGVTLS
                     HASPPPAPIPTRIGFHYFNLGKEGVFWEAIRNSKTVAVYVPQEFVGQKLELYAVKP"
     gene            complement(5767..7329)
                     /locus_tag="VF240_08580"
     CDS             complement(5767..7329)
                     /locus_tag="VF240_08580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7175298.1"
                     /translation="MTTYDEVLYPSHSFAQAHPDRLATQALIFGMSPAPLERCRVLEL
                     GCGDGGHLIPVAYNFPGSHFVGVDLAARPVEKGRAIIADMGLENIRLLQMSIVDFPAD
                     AGVFDYVIAHGVYSWVPPDVRDRVLSICGEHLAPDGVAYVSYNVLPGGHLRRMTREMM
                     LWHAGDAGDPAERVARAQGLLETLAGTLDGSEPFAALLKEEVERVRRFLPAHLFHDDL
                     SEVNAPIYFHEFVEHAARHDLQYLAEADYSDMATHSLSGQTVEALRRLAGDDLLRLEQ
                     YADFIKCRRFRQTLLCRRGVERAEERQPDVVRRLYVSSPLSPKSAEPDVRSRAVEEFW
                     GVGAGALSTDSPLVKAAALIMGETWPRSVSFGELLSAARLRAGTDGDESTLEEDAQRL
                     SVILLDACETGVVKLHAHRPNFVTRVSERPRASGLARAQLRHGSLVTAQSHANVDIKD
                     ALGRALLLLLDGTRDRAALLAELSARIRSGDIDVGDAAAGAPTPEQLLDALPAGLERS
                     LEGLANSTLLVA"
     gene            7633..9066
                     /locus_tag="VF240_08585"
     CDS             7633..9066
                     /locus_tag="VF240_08585"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7175299.1"
                     /translation="MLSAKLIVSQPGQRSHSVAVAGPSVSVGRRPDNTICLDDTNVSK
                     YHAVIEWRDDAYWLSDLGSSNGTSVNGERLGSTRRLRNNDLVSLGGTSTLQLFIDERQ
                     AAEAQHNPTAVGTAPAGLSMPASAGVPAMPQMPHMPHVPQAPHVPHMPQAPAMPVVPQ
                     PPAAVQKILGMPRALAASLAGLLAVGGLLGVLWGTGVIGNSSAKTSKGKAGRANERGE
                     ANAAADSTGKGATGETGATTGRATTTGTAAEAATPPVVSGGGAAAGAGATPALARTLA
                     VQISQKSAYNFDPGFAALINTYVNEYRAAAGYSERAAKHREAIEREFVNVQGIQPPLV
                     AYVLAMSQTKFVERPGQGIWNLPPAVVRAHSTGDAPADMSDPAAGARVAAAYVRSLLD
                     LFERENFMYAVACYGMTLDEAGKVRVALESKDPTGQGRTDFWKMKNAGVVQGAQVERV
                     ARFFAAGIVCENPAQYGLKEKPFSSLY"
     gene            9214..10674
                     /locus_tag="VF240_08590"
     CDS             9214..10674
                     /locus_tag="VF240_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7175300.1"
                     /translation="MPHVTLTINSPGGASEVRLNGDALTFGRTDAAGIRIPEDAGLSR
                     RHATVRRDGARVWIVDEGSTNGSFVNDARVSPEGLSLSDGDQIRIGHSTTIAVHIIKD
                     EPAATRDAPSGAQATPPAPAADESAPTTTPAASLPLKVLVPVLAVVVIVLGAVGFLLY
                     QLTRPTETRSAAESDRASETRRPESAAGATPERTGPASQETPTPAPVVKVREDISILS
                     AAPNATTVAPVVEVAPAPAQRRLYKEMAEAEKTEFIKRRAQHVATMMGRRPYAFTPEA
                     LTHVKFWLDAFARRVGNNRTGLWSGDTVAILERGRTHAPTIVRAFRDNNVPVIVGLYI
                     PFIETEYTNITTNNFAGAAGLFQFLGPTAEHYGVPSAERTDVAKMAPAAARYFRDNIM
                     SFGDDPMSVALSIAGYNRAPESVKRDLRNVLNAPNNEDKERSFWTLIANQGKLDEYFQ
                     RENVNYVPRFFAAAIIGETPWAFGIEMRPLSTYTEQ"
     gene            10799..12166
                     /locus_tag="VF240_08595"
     CDS             10799..12166
                     /locus_tag="VF240_08595"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7175301.1"
                     /translation="MPSQINLIYTDDGGEPRRIALRSPRLSVGRGADNDLCINDTNLS
                     RRHAIVEVIAGRPYISDCGSSNGTTVNGQTVTSAIELYDGDRINLGNSRELIVAIEEA
                     TAATDDAVAASATPDANAQAGSPEAGQVSGPAAPASPHAPPAGSASEKVAATALRAPI
                     LAGAAIGVILLGALVLFVYSLGGDDKKTRGGDGRAGASNTGVERANATGGRTPGGNAG
                     PRETPRAETNASVNAQASEVASGTPSAATAGDNTDEQIGREVRRAMGRISSDNAPYIS
                     ETGAADVARKVREYRGSAALAERLRALARGCADVTTLARTNNLKPSLLSYAALAQAES
                     AGGGDPVAVARQMSPKLLTLRATFGTETANSSLLLVAAYPYPFNPSIGSQERTPHPLA
                     SKLMEVGGRRSVVETSVARSVWFLREKNAVSAEAYDLVVRLLAVGVIAQNPRQYGVEA
                     DPVFC"
     gene            12334..12942
                     /locus_tag="VF240_08600"
     CDS             12334..12942
                     /locus_tag="VF240_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175302.1"
                     /translation="MAVNVHEVFARGGKGLIDLLEFCVISVRMDPVFVFLAGEFRTHP
                     TARGAVALYELFCTRDAPARVSALRALPPYNLRLPAAVRPLAESLERMRAARDAEAVV
                     LAETEEEDGGEAGGVEPPGELPPPLFPARYIFDEVVRQLESGRGQLREVGELYDPTLT
                     PYENLPGRRMTPAQKAFVDRVWAPRVRPRLVAAGFRRIADIA"
     gene            complement(13114..14781)
                     /locus_tag="VF240_08605"
     CDS             complement(13114..14781)
                     /locus_tag="VF240_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175303.1"
                     /translation="MKSAKIFFLLVLFAACPRAQETGARATGPVSAAEAARWREDIRY
                     MAEEMPRYHANLFHTLSREQFEAAVRGLHERVPQLARHQIIVEMMRVVASVGDGHTNI
                     APTRDPKVGFRALPVKLYLFKDGLFVRAATHEHAGLVGARVLKIGGVSADEAIARARE
                     IIGRDNEMGVRFFAPFLLAMPEVLHALGLADDAEGARFVVEAELRQQSVTLRPAGPVE
                     MLPADTDTSWLPKDGWVDMRDGAKAAPPLWLKEAREEFWFEHLKETRTLYVQLNKVGD
                     KKGESLADFTKRLFAFVEANEVERFVLDLRLNRGGNGELLTPLVRSIIKSKIDRPGKF
                     FVITGRGTWSASQFLIDHLEKYTDAVFVGEPSGSKGNHYGDSRRITLPNSGLTVRVSI
                     YYWQHWHPIDTRPWSAPEVTAELSSEDYRAGVDPALRAVLGYVPRRNLTEILDEALTA
                     GGAELAVKRFREFKAEPANKYADTEESLLIAGFRLLQENKPEQALALFKLCAEENPHS
                     FRAHFAVGEAHLRGANKALAAESFEKALRINPKYYEAAERLKQLRRK"
     gene            complement(14980..15873)
                     /locus_tag="VF240_08610"
     CDS             complement(14980..15873)
                     /locus_tag="VF240_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175304.1"
                     /translation="MEKITYLNLPNCYRLSNGDAEVVVTTDVGPRVVRYCLAGGENVL
                     GEVPGASVATPLGEWRPLGGHRLWTAPEAMPRSYVPDNDAVEFEVIDELSVRLRQQPE
                     QATGMQKEITVALDAEGSGVRLLHRVVNRNLWGVELAAWALTIMRGGGQAIIPQEPYG
                     PHPACLLPARALVLWPYTNLADARWSLGPKYIRLATDATASAPQKLGVSNRRGWAAYA
                     VDDTLFVKHFGYEEGASYPDFGCNNEVFTAGSFIEVESLSPLRSLGPGEAVEHAERWR
                     LFGGVRVGREEADVERVVGRW"
     gene            complement(16053..16853)
                     /locus_tag="VF240_08615"
     CDS             complement(16053..16853)
                     /locus_tag="VF240_08615"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX7175305.1"
                     /translation="MTTGRYRSSEIESFVGQVLRDGFCVLPSHFERARLEAWGEAFAP
                     LLERHLEREGHVQNRGPLRYYVTLPFRQPFSDPRVFEDEDVLGVVMGLVGEDAVMCQL
                     ATDTPLLGSDYQDVHRDAPPLFPETGSETPPFQLAVNFPLADVTPENGPFEVARGTHL
                     LSKEEGLRRIEAGETALEPILLRAGDVMIRDVRGLHRGTPNRTARPRPMVVVGYSRRW
                     LFRPEVSIRVPRDALSALSERARFLLRFNPVVESVDDEGTEIYQSFAY"
BASE COUNT         2925 a         5635 c         5666 g         2842 t
ORIGIN      
        1 gagcggcaga cgcaggacgc gcgcggcgtc gccgctcgcc ggcgaggagc tgaagccttt
       61 ggcttcgagc gagtcggcga ccgcctgacg cgcgggccgc aggccgatgg tatcgtcgcc
      121 ggcctgcatc gccttcgaga gttccgagag cggcttgttg ttggcgttta aggcgtcggc
      181 caccttcttg agttgcgcgc ggtgctcggc catcggcgaa gcgtccgcct tgcccgagac
      241 gaattgcacg agcgggcgaa actccttatc gacgggggtg acgccgatgc cggtcttcga
      301 cgtgaagaga cctttgagcc acgccatgac gccgtcgccg tccgcgcccg agaggttcgt
      361 ctgccgcgag acctcttgca gcagactctc cagcggcgac gtgctccccg agagcaggcg
      421 caagcttcgg acggcgtcct ctttggattg gaagtcgcga atcttcgtct cctgaaggaa
      481 gcgttgccag tgcgcgacgt agtcgcggta gtacatgttg gccagctcgt ccttcttggc
      541 gtcgatgttc tcgccggcgg cgaggctggt acgcatcacc caatcatcct tgcggaactc
      601 gtcggcgacg gacgacttca aacgctcggt caactcggtg tggccgtcgc gcgtgaaggc
      661 gccgggcacc gagtaggtgt tgttgatgac gccgccctcg cgcgcgttcg ggatggaggc
      721 gagcttgacg ggaaacttga cggcgccgtt gatctcggag gtgatgcgct tgtagacgcg
      781 gttgacgacc gggtaggcga cgagcttgtt ctgcgccccg tcgatgagcg agttgtcgat
      841 ctcggggtgc ggcgcgtcct cgcggcccgc ctgtgtggag aagtattcga gctgccggat
      901 ggagacctcg gcggtgccag cgggcgcgac ctcctcccag tattcgcgaa ggacttgcgc
      961 gagaaaggtc ggctccacct tgtcgggctt cgagagcatc agatacgcct tgagcaggtc
     1021 gtagtgtcgg ccgagatagt cttcttccgt cgaggccgag gtggcggaag ccagcgccgc
     1081 ctgcgacgac gagccggggg tcgcggcagg agaaggcgtc ggccgcgtcg tggccgtcgt
     1141 cgcggggccg cccgtcgggc cgctcctgcc gaactcgcgc aagtcttctt ccatcttggc
     1201 gaccgccggc ttcaggaagc ggtcttcaat cgcctcgaag tagatgtggc ggaggatgga
     1261 tgggcttgcc gggtctgacg agttgagctt gctgcccgcg tagagtccga agcgcatcag
     1321 gacgggcggc gagttgcgct cgtgctcgtc aagttcggca agcacctctc gcacgtcctc
     1381 gacggcgcgg agttcgtcgg ccacctgcgc ctcggtggcg cgcccgcggc tcgtcgcctt
     1441 cctgatgtcg gtcaggcgtc ggccgcgcgc gtcggcgtcg gcgatcagac gtttgttggc
     1501 gaagaaggag acgaccatcc cgccgacgag aaagaggaga agggccgcca gcgcgccgaa
     1561 catgatattg cgtcgcaggt gcgggctgcg gcgggcggcc tgcgcggcgg cgaccacgtt
     1621 gcggtcgggc agcagcacgt cgcggaagag gctctgcgtg aagtactcgc cgccctgcaa
     1681 ctgcttggcc ccggtggggc cggcgcccgc gctgctcgtg aagtagacgc cgcgcaggag
     1741 cgggctttcg ctgaacgggt tggggcggaa aagcgcggtg gcgaaagcgc cgagacgggc
     1801 gcgcgtgcgg cggaagcgcc ccggaaactt gaagacgcgc aactgcggct cgggcttcgc
     1861 gctaacccca agttgcacca tgcgtcggcg cagcaggcgg ccgtagagat attcaaactc
     1921 cgcgtcgaac tgcgcctgcg cgccgtcgcc ctgcgcgagc gggaaggtcg aaccccacac
     1981 ctgcgcgcgc tccgggggcg cgaacgagtc gaagaactcg ccgaagccct cgacggcgtc
     2041 catgtgcgtg aagacgaggt agacggggaa gcgcgcgccg gcgcgggcca tcgcctcgtc
     2101 gagtcgcgcg cgcagcacct tcgcctgctg ctcgatctgg gcctcgttca tccgcaacag
     2161 gtcggcggcg ttggcggcga ggacgaagcc gtcaacgggc cgcgtgcggc gatggcgctt
     2221 aacggtctcg atgagcgccg cccactcgtc gcgctccggc ccctcggtct ggtagcggcc
     2281 cgtggtgtcg agccagacgg ccgagtctgt caccttccag tcgcagtgct cggtcggtcg
     2341 caccactctc tgctcggagg cgcgctggct gggcaggacg tggaagtcga gtcccgagga
     2401 gagcaggagc gaggtcttgc cgctgccgcg cgggccggcg acgacgtacc acggcagggc
     2461 gtaggcggcg tcggcgctcg cgccgccggc gagcttgctg ccgcgcagcc attgcactac
     2521 ttcctcagtc ccgcgcgtca gctcctcgta agtgcccgtg ggcggcggca gcgacttcgc
     2581 ccccttcccg ccgttctgcg ccgcgtcgga gccgccgccg tctgcggcgc gcgagcggcg
     2641 gaagtgtttg aggagcaccc acgcgggcca cgccagcaag acgagcgcga cgaggaggat
     2701 tttgacgggt gtggaggccg gaaccagaaa cagcacgagc agcaccaaca ggccgacgag
     2761 aaaaatcttc ccggcgatca ggagtgccgt cttcacctgt gtgcccgtgg atgacatcga
     2821 actgacgtta ccgccccgtg ctccgtcgcg ctctagtggc ccgcgccggc ggccggagac
     2881 gtgtccagat tactcgaagg ctcggtgttg aaaaccgcca tcgcgaattc ggtgagcgcc
     2941 ggatgcgctc ccgccgagga acgtgcgcgc gaggttgatg tggaatccaa cccaaccgtt
     3001 tgggcgcggg gcgtgcgtga atcgcttgtc gggtcttaaa gtgcaatcgg gtaaacgcgg
     3061 cttaaggtta aattaagaaa acgttaacac ttctgcgggt tgagccggac attcaagaag
     3121 aagaactatg gtgacgggca tgctacctcc cgccccgcgc actgtcaaga gcgacccgtt
     3181 tttaccttac gatgttcagc tctcgctgga gcaacatgta gagcacgagg taggtgagga
     3241 gggcgaagac gaggagcgcc gggccgccga tcttggccca caggggccag cccggatcgc
     3301 gcggcggctc cgggtggtcg gccgcctgcc agtggccgga gagcgcgttc ggcttcaggc
     3361 gtccggccgc gcgcagccgc tcggccacac gggcaatcgt ctccttcaac tgctcttcca
     3421 gcaggtagac gttgtacttg cccttgaagc cgagaagcag gcagagatag tagagttcga
     3481 tgacctcgac gttggcctcc aaatccttga acatctcttc gagccggtcg aagaacacca
     3541 tgcccgccat gtgctcctcg aagtagacga gttggagggg ctggccttcc cactgctggc
     3601 ggagcgggaa gttgtgtttg gggctgagca cggtctcatc gacgaaggcg acgagggcga
     3661 acttcacgtc gcggatgcgg cgcgggtcgc agcgcagttg cacgccgccc tcttcgagct
     3721 gccgcagcag gccgtccatc accccgcgga tgtcgttcga cggctcgatg gcgcccgagc
     3781 gaatcttcaa caccagctcc agcaccggcg ctgacagatt gatcaggtcg ttcgtcgtcc
     3841 gctggcggcg gcgggccgcg gccgcggcgg gcatctcggc gactgcactc ataagcttcg
     3901 cgtgataagt gatgagtgat gagtgataag aaagagaaga caggttttgt cttatcactt
     3961 atcactcatc acttatcacc gtcctcatgg tttgaccgcg tagagttcga gcttctggcc
     4021 gacgaattcc tggggcacgt agacggcgac ggtcttcgag ttgcggatgg cttcccagaa
     4081 gacgccctcc ttgccgaggt tgaagtagtg gaagccgatg cgtgtgggga tgggcgcggg
     4141 cggcggcgag gcgtgcgaca gcgtcacgcc cggcagccct gagccgatga cgccgtcgat
     4201 cacgtcgcgc gaggcgatct tgacgatgcg cggcacgcgc tcgatgagct gcccctcggg
     4261 caccttggcg ctcaccccga gatagaactc cgccgtgtcg agcagccgct cgtcctgcac
     4321 gcggccgacg taaacgctct cgcgcgtgct ctccagcgga atctgtacgg agcgcgtcgg
     4381 gatgacggtc tccaacaggc cgcgcagctc ggcggccaac tggctgaagg tcgcgtagag
     4441 gtcggtgtgc tggtagtgga cgatctgctg cggctggcgc tcggtcgaga aggtcatcag
     4501 cttgccgcac agctcggcca tctccgtgta gagccgttcg gggtggacga gacgcgtgcg
     4561 gaacaggtgc gcgagcacgg ggatcgaggt gttgaccgtg tgcagcagcc agaagacggc
     4621 gatctcggag gtggtgaagt cggcgagcga agtggtgcgc tggcgccgct gatcgctcat
     4681 cgagctgctc ttggcgatca ggatttcgat gatctgccgg agcatctggc cgagccacgg
     4741 cgaggaggag acggacagcg ccggcgggac gtagtttgcg ttgtaggtca actgccccgt
     4801 cgtggtgcgg cggacttcgg cgatcttgac ggagttgtaa ccgtcgcgca gctccgaggc
     4861 gaacatcagg cgcaggttgg ggcgcgcgaa ggctagttgc tgctcgccct gccccgtcgt
     4921 ttcgtcctgc acggtggccg cgtcctgcca gtagcggacg aactggctgc gctcgctgcc
     4981 gttcgactgg aagttggccg cgccggctct cttggcgggg acggcgaggt gtacgtcgag
     5041 actttcgtcg ccggcctcga aatggtcgcc gacctgacgc gggtcggggg cggcgtccat
     5101 ctgcgggatg ttgacgacga gtccgtcggg gagcacggcc cggcagcgcg cgacctcgca
     5161 gaagccgttg gcgatggcct cctgactgag ctgcaagtcg agcacgcccc actcgtaggg
     5221 cgagagcgag gcgaggcgcg agttcaaaag ctcctcgtag tagttgtccc actgctggaa
     5281 gtgatgaggg gtcagaagca tcccctcgtt ccagacgatt ttacggtaca cgctcatagt
     5341 taaagggcac gaatcccccg cggcgagtct ttgaagtccg atgcaaaggc cgggggcggt
     5401 gtgttcaggc gactgccttt cgcggcggca agacggactc gggacagcgt caattttaaa
     5461 aacggaacgt cgaaaatagt gcgcgcgcgg gcgccaggct gatgtgcccg attgtgttac
     5521 ctcgcgccat gtttgtcaac acacccccac gcgacgagtt cgagttccga ttcaacaatc
     5581 ggcttccgct gcgccatctc cgccgacgac gcccgtctca agacgctgat gcaaaagaag
     5641 tgataagtga tgagtgataa gtgatgagaa gctgttagcc cttagcgatt agctaacagc
     5701 caacagctaa gagctaacag cttcttttct ttcttatcac ttatcactca tcacttatca
     5761 ctaagttcag gcgacgagca gcgtgctgtt cgcgagtcct tccagactcc gctccagccc
     5821 ggccggcaac gcgtcgagaa gctgttccgg tgtgggcgcg cccgcggccg cgtcgcccac
     5881 gtcgatatcg cccgagcgga tgcgcgccga tagctccgcg aggagcgcgg cgcggtcgcg
     5941 cgtgccgtcg aggagcagca ggagtgctcg gcccagcgcg tctttgatgt ccacgttggc
     6001 gtggctctga gcggtgacga gcgagccgtg ccggagctgc gcgcgcgcca gaccgctcgc
     6061 acggggccgc tcgctcacgc gcgttacgaa gttcggccgg tgcgcgtgca gcttgacgac
     6121 gcccgtctcg cacgcgtcga gcaggatgac gctgagccgc tgcgcgtcct cttccaaagt
     6181 actctcgtcg ccgtccgtgc cggcgcgcaa gcgcgccgcc gacagtagct ctccgaagct
     6241 gaccgaccgc ggccacgtct cgcccatgat taaggccgcg gccttgacga gcggcgagtc
     6301 ggtcgagagc gcgccggccc cgacgcccca gaactcctcg accgccctcg acctcacgtc
     6361 cggctccgcc gacttcggac tcagcggcga cgacacgtag agtcggcgca ccacatccgg
     6421 ctgacgctcc tcggcgcgct ccaccccgcg ccggcacaaa agcgtctgtc gaaacctgcg
     6481 gcacttgatg aagtccgcgt actgctcaag ccgcagcagg tcgtcgcccg cgagccggcg
     6541 cagggcctcg accgtctggc ccgagagcga gtgtgtcgcc atgtcggagt agtcggcctc
     6601 ggcgagatat tgcaaatcgt gccgggcggc gtgctcgacg aactcgtgga aatagatggg
     6661 ggcgttaacc tcggacagat cgtcgtgaaa aagatgcgcc gggagaaagc gcctcaccct
     6721 ctcgacttct tccttgagca gagcggcgaa cggctctgag ccgtcgagcg tgccggcgag
     6781 agtttcgagc agcccctgcg cgcgcgcgac gcgctcggcc gggtcgcccg cgtcgccggc
     6841 gtgccagagc atcatctcgc gcgtcatccg ccgcaggtgc ccgcccggca gcacgttgta
     6901 gctcacgtag gcaaccccgt caggcgcgag atgttccccg cagatcgaca gcacgcggtc
     6961 gcgcacgtcg ggcggcaccc aagaatagac gccgtgggcg atgacgtagt cgaacactcc
     7021 cgcgtcggcg ggaaagtcca cgatggacat ttggagcagg cggatgttct caagacccat
     7081 atcggcgatg attgcgcggc ccttctcgac ggggcgcgcg gccagatcga cgccgacgaa
     7141 gtggcttccg gggaagttgt aggcgaccgg gataaggtgg ccgccgtcgc cgcagccgag
     7201 ttcgagcacg cggcagcgct ccagcggggc gggactcatg ccgaagataa gcgcctgcgt
     7261 ggcgaggcgg tcggggtgcg cctgcgcgaa cgagtgcgac gggtagagga cttcatcgta
     7321 ggtggtcatc ttaaaaggcc gtgaatcgtc aatcgtgaat cgtcaatcgt gaatacagtt
     7381 agaacgtcat tcgactctcg tcgtcactcg tcaggctaag cggcatcaac cacgattgac
     7441 gattgacgat ttttcatgag cgcggcgagg gtttccgaaa gctctgcggg gacgacgcgc
     7501 ggaggcgttc gcgttgcgcc gtcttgcctg cctgtgtggt tcgggtaaaa taggcgcacg
     7561 cgacaagtcg gccgcagcag caagtgcgcg acggtcgcgg ccgctgcggc tactcccgca
     7621 atcgcctccg tgatgttatc agcgaaacta atcgtcagcc agccggggca aagatcgcac
     7681 agcgttgcag tcgcggggcc gtcagtctcc gtcggccggc ggccggacaa caccatctgc
     7741 ctcgacgaca cgaacgtctc gaagtaccac gcggtcatcg agtggcgcga cgacgcctac
     7801 tggctgagcg acctcggcag cagcaacggc acgtccgtca acggcgagcg actcggttcg
     7861 actcgccgcc tgcgaaacaa cgacctcgtc agcctcggcg gcaccagcac cctccaactc
     7921 ttcatcgacg agcggcaagc ggcggaagcg caacacaacc cgacggctgt cggcacggcc
     7981 cccgccggcc tctcgatgcc cgcgtcggcc ggcgttccgg ccatgccgca gatgccgcac
     8041 atgccccatg ttccgcaggc gcctcacgtg cctcacatgc cgcaggcgcc cgcgatgccg
     8101 gtcgtaccgc aaccgccggc cgccgtgcaa aagattttag ggatgccgcg ggcgctcgcc
     8161 gcgagccttg ctggtttgct cgccgtcggc gggctgctcg gagtgttgtg gggcacgggt
     8221 gtgatcggca attcgagcgc gaagacctcg aagggtaagg cgggacgcgc aaacgagcgc
     8281 ggcgaggcga acgcggcggc agattctact ggcaaaggcg cgaccggcga gacaggcgcg
     8341 accaccggcc gggcgacgac tacggggacg gccgccgaag cggccactcc acccgtcgtg
     8401 tcgggcggcg gcgcggcggc cggggcgggc gcgactccgg cgctggcgcg cacgctggct
     8461 gtccaaatct cgcagaagag cgcctataac ttcgaccccg gcttcgccgc gctcatcaac
     8521 acctacgtca acgagtatcg cgccgccgcc ggctacagcg agcgcgccgc gaagcaccgc
     8581 gaggccatcg agcgcgagtt cgtcaacgtg cagggcatcc agccgccgct cgtcgcctac
     8641 gtgctcgcga tgagccagac gaagttcgtc gaacgccccg gccaaggcat ctggaacctg
     8701 ccgccggcgg tcgtgcgcgc acactccacg ggcgacgcgc ccgccgacat gagcgacccc
     8761 gcggcagggg cgcgcgtcgc cgccgcctac gtccgctcgc tcctcgacct cttcgagcgc
     8821 gagaacttca tgtacgccgt ggcctgctac gggatgactc tcgacgaggc cggcaaggtg
     8881 agggtggcgc tcgagagcaa agaccccacg gggcagggcc gcaccgactt ctggaagatg
     8941 aagaacgccg gcgtggtgca gggcgcgcag gtcgagcgcg tcgcgcgctt cttcgccgcc
     9001 ggcatcgtct gcgagaaccc cgcgcagtac ggcctcaagg agaagccctt ctcctcgctc
     9061 tactgaaggc gcgtaaaaac aggtgataag tgatgagtga taagtgatga gaaagaaagg
     9121 gagctgatag ctcttagctg tcggctatta gctaatcgct aagagctaat agcttcttat
     9181 cacttatcac tcatcactca tcactcccac gaaatgccac acgtcacgct cacgatcaac
     9241 tcacccggcg gcgcgagcga ggtgcgtctg aacggcgacg cgctgacctt cgggcggacg
     9301 gacgccgcgg gtatccgtat ccccgaggac gcggggctgt cgcgacgcca cgccaccgtg
     9361 cgccgcgacg gggcgcgcgt ctggatcgtg gacgaaggct cgaccaacgg cagcttcgtc
     9421 aacgacgcgc gcgtctcccc ggagggactg tcgctctccg acggcgacca aatccgcatc
     9481 ggccactcca ccaccatcgc cgtccacatt attaaggacg aaccggccgc gacgcgcgac
     9541 gccccttcag gagcgcaagc cacgccgccg gccccagccg cggacgagtc ggccccgacg
     9601 acgacgccgg ccgcgtccct gccgctcaag gtgctcgtcc ccgttctcgc ggtcgtcgtc
     9661 atcgtcctcg gcgcggtggg ctttctcctt tatcagttga cgcgaccgac cgagacgcgc
     9721 tccgccgccg agagtgatcg cgcgagtgag acgcggcggc cggagtcggc cgcgggcgcg
     9781 acgcctgagc gcacggggcc ggcttcgcag gagacgccga cgcccgcgcc cgtcgtcaag
     9841 gtgagagaag acatctcgat tctctccgcc gcgccgaacg ccacaaccgt agcgccagtt
     9901 gtggaagttg cgcccgcgcc cgcgcagcgc cgtctctaca aagagatggc cgaggcggag
     9961 aagacggagt tcatcaagcg gcgcgcgcag cacgtcgcga cgatgatggg ccggcgcccc
    10021 tacgcgttta cgcccgaggc gctcacccac gtcaagttct ggctcgacgc cttcgcccgc
    10081 cgcgtcggca acaatcgcac gggtctgtgg tcgggcgaca ccgtcgccat ccttgagcgc
    10141 ggccgcacgc acgcgcccac aatcgtccgc gccttccgcg acaacaacgt gcccgtcatc
    10201 gtcggcctct acatcccctt catcgaaacc gagtacacca acatcacgac gaacaacttc
    10261 gccggcgcgg ccggtctctt ccagtttctc ggcccgacgg ccgagcacta cggcgtccct
    10321 tcggccgagc gcacggacgt ggcgaagatg gccccggccg ccgcgcgcta cttccgcgac
    10381 aacatcatga gcttcggcga cgacccgatg agcgtcgcgc tctcgattgc cggctacaac
    10441 cgcgcgcccg agtccgtcaa gcgcgatttg cgtaacgtgc tgaacgcgcc gaacaacgag
    10501 gacaaggagc gcagcttctg gacgctcatc gccaatcagg gcaagctcga cgagtacttc
    10561 cagcgcgaga acgtcaacta cgtcccgcgc ttcttcgccg ccgccatcat cggcgagacg
    10621 ccctgggcct tcggcatcga gatgcgcccg ctctcgacct acacggaaca ataaggcggt
    10681 gataagtgat aagtgataag tgatgagtga taagtgataa gtgataagtg ataagtgagt
    10741 gcagacgggc tttgtctttc ttatcactta tcactcatca ctcatcactc caacaaagat
    10801 gccctcgcag ataaatctga tctacacgga cgacggcggt gagccgcgcc gcatcgcgtt
    10861 gcgaagcccg cgcctctcgg tcgggcgggg cgccgacaac gacctctgta tcaacgacac
    10921 gaacctctcg cgccgccacg ccatcgtcga agtcatcgcc ggccgcccct acatctccga
    10981 ctgtggttcg agcaacggca cgaccgtcaa cggccagacg gttacgtcgg ccatcgaact
    11041 ctacgacggc gaccgcatca acctcggcaa ctcgcgcgag ctgatcgtgg ccatcgagga
    11101 agcgacggcg gcgacggatg acgctgtcgc cgcgtcggcc acacccgacg caaacgcaca
    11161 ggccggctcg cccgaggccg ggcaagtgtc cggccccgcc gcaccggctt ccccccacgc
    11221 gccgcccgcc ggctctgcgt cggaaaaggt cgcggccacg gcgctacgcg cacccatcct
    11281 cgccggcgcg gccatcggcg tcatcctcct cggcgcgctc gtgctcttcg tctactctct
    11341 cggcggcgac gacaagaaga cgcgcggcgg agacggccgc gccggcgcat ccaacacggg
    11401 cgtcgagcgc gcgaacgcca cgggcgggcg gacgcccggc ggcaacgcgg ggccgcgtga
    11461 gacgccgcgc gcggagacga acgcctccgt gaacgcgcaa gcaagcgaag tcgcgtcggg
    11521 cacgccttcg gcggcgactg cgggcgacaa cacggacgag caaatcggcc gcgaggtcag
    11581 acgcgcgatg ggccgcatca gctccgacaa cgcgccctac atctcggaga cgggcgccgc
    11641 ggacgtggcg cgcaaggtgc gcgagtatcg cggctccgcg gcgctggccg agcgtctgcg
    11701 cgcgctcgct cgcggctgcg cggacgtaac gacgcttgcg cggacgaaca acctcaagcc
    11761 gtcgcttttg tcctacgccg cgctggcgca agccgaaagc gcgggtggcg gcgaccccgt
    11821 cgcggtcgcg cgccagatgt cgccgaagct gctcacgctg cgcgccacct tcggcaccga
    11881 gacggccaac agctcgctcc tgctcgtcgc cgcctatccg tacccgttca acccctccat
    11941 cggcagtcag gagcgcaccc cgcacccgct cgcctcgaag ctgatggagg tgggcgggcg
    12001 tcgctccgtc gtcgagactt cggtggcccg aagcgtctgg ttcctgcgcg agaagaacgc
    12061 cgtcagcgcg gaggcttacg acctcgtcgt gcgcctgctg gccgtcggcg tcatcgcgca
    12121 aaacccacgg cagtacggcg tggaggccga ccccgtattc tgttgaggcg cgtggcgtgt
    12181 gacgcgtttt agaagtgcta cggcgtcatc cacttcagcc acgcacggac tgctcgacgc
    12241 gcgccagccg cagttcacca gtctggtgct gtcgctcgtc agggaacctg aaggtgagag
    12301 tcactctaaa cgaatagttg agagatttag actgtggcgg tcaacgtcca tgaagtcttc
    12361 gcgcgcggcg ggaagggtct gatcgatctg ctggagttct gcgtcatctc cgtgcggatg
    12421 gacccggtgt tcgtcttcct cgcgggcgag ttccgcacgc acccgaccgc acggggggcc
    12481 gtcgccctct acgaactctt ctgcacccgc gacgcgccgg cccgcgtcag cgccctgcgc
    12541 gcgctccccc cttacaatct ccgtctcccg gcggccgtcc gcccgctcgc ggagagcctg
    12601 gagcggatgc gcgccgcgcg ggacgcggaa gcggtcgtgt tggcggagac ggaggaggag
    12661 gacggcgggg aggcgggagg tgtcgagccg ccgggagagt tgccgccgcc gctctttccc
    12721 gcgagatata ttttcgacga agtcgtccgc caacttgagt ccggccgggg gcagcttcga
    12781 gaggttggcg agttgtacga cccaacgctt acgccatacg aaaatttgcc gggccggagg
    12841 atgacgccgg ctcagaaggc gttcgttgac cgcgtctggg cgccgcgcgt caggccgcgc
    12901 ctcgtcgcgg cgggcttccg gcgcatcgcc gacatcgcct gaagctgtgc gccgcgtcat
    12961 cgtcgtaaaa aaagagaggc cgcggaggtt cgtctctccg cggcctctct tcagttgagt
    13021 atagggtagt tgagattcac cgccggggcg cagtttttga tttcgtgacg ggctcgacgg
    13081 tcgggacacg cctcaacaca cgttgtctgc cctctacttc cgtcgcaact gcttcagcct
    13141 ctcggccgct tcgtaatact tcgggttaat tcgcagagcc ttctcgaagc tctcggccgc
    13201 gagcgccttg ttggcgcccc tgaggtgagc ttcgccgacc gcgaagtgtg cgcggaagga
    13261 gtgcgggttc tcttccgcac aaagtttgaa gagggcgaga gcttgctctg gcttgttctc
    13321 ttgcagcaga cggaagccgg cgatgagcag cgactcctcc gtgtcggcgt acttgttcgc
    13381 tggctcggcc ttgaactcgc ggaacctctt cacggccagc tcggcccccc ctgcggtcaa
    13441 cgcctcgtcc agaatctccg tgagattcct gcgcgggacg tagcccagca ccgcccgcaa
    13501 cgccggatcg acgcccgcgc ggtagtcctc cgacgacagc tcggccgtca cttcgggcgc
    13561 cgaccacggg cgtgtgtcta tggggtgcca gtgctgccag tagtaaatcg agacgcgcac
    13621 ggtcagcccg ctgttcggga gcgtaatccg ccgcgagtcg ccgtaatggt tgcccttcga
    13681 cccggaaggc tcgccgacga aaacggcgtc cgtatacttc tccagatggt cgatgaggaa
    13741 ctgcgaggcc gaccacgtgc cgcgccccgt gatgacgaaa aacttgccgg gtcggtcgat
    13801 cttcgacttg atgattgacc tgacgagcgg cgttagcaac tcgccgttgc cgccacggtt
    13861 gagccgcaga tcgaggacga accgctcaac ctcgttcgcc tcgacgaacg cgaacaggcg
    13921 cttcgtaaag tcggccagcg actcgccctt cttgtcaccg accttgttga gctggacgta
    13981 gagcgtgcgc gtctccttca ggtgctcgaa ccagaactct tcgcgcgctt ctttgagcca
    14041 gagcggcggc gcggccttcg ccccgtcgcg catgtccacc cagccgtcct tcggcagcca
    14101 gctcgtgtcc gtgtccgccg gcaacatctc gacggggcct gccggccgca gcgtgaccga
    14161 ctgctgccgc agctcggcct cgacgacaaa gcgcgcgccc tccgcgtcgt cggcgaggcc
    14221 gagcgcgtgg agcacctcgg gcatcgcgag caggaaggga gcgaagaacc tcacgcccat
    14281 ctcgttatcg cgcccgatga tctcgcgcgc gcgggcgata gcctcgtccg ccgagacgcc
    14341 gccgattttc aagacgcgtg cgccgaccaa gccggcgtgc tcgtgcgtgg cggcgcggac
    14401 aaacaggccg tctttgaaga ggtagagttt gacggggagc gcgcggaagc cgaccttcgg
    14461 gtctcgcgtc ggcgcgatgt tcgtgtggcc gtcgccgacc gacgccacga cgcgcatcat
    14521 ctcgacgata atctggtgcc gcgccagttg cggcacgcgc tcgtgcagcc ccctgacggc
    14581 ggcctcgaat tgctcccgag acagtgtgtg gaagaggttg gcgtgatacc tcggcatctc
    14641 ctcggccatg tagcggatgt cctcgcgcca cctcgccgcc tcggccgcgc tcaccggccc
    14701 cgtcgcccgc gcgcccgtct cctgtgcgcg cgggcaggcg gcgaacaaca cgagcagaaa
    14761 aaatatcttt gcactcttca tataaacgcg ctgcgactcc ctttctcgaa ctcgcgtgca
    14821 gtagcggcgc gtgtgacctc cgccgcgcgc agcttggctt agacataaaa cagttgagcg
    14881 tgcggaaagt tacagcagcg tcttctctca atcgagggca tagggtatag gcagttcacc
    14941 gcgtcctccg cggtgaatcc gacttagcga acactcaact caccaacgcc cgacgacgcg
    15001 ctcaacatcc gcctcctcac gtccgacgcg cacgccgcca aagagacgcc atctctccgc
    15061 gtgctccacc gcctcgcccg gccccagact cctcagcggc gacagcgact ccacctcgat
    15121 gaacgagccg gccgtgaaca cctcgttgtt gcacccgaag tccgggtagc tcgcgccctc
    15181 ctcgtagccg aagtgcttga cgaagagtgt gtcatccaca gcgtaggccg cccacccgcg
    15241 ccggttggaa acgccgagct tctgcggcgc ggaggctgtg gcgtccgtcg ccagccggat
    15301 gtacttcggg ccgagcgacc agcgcgcgtc ggcgaggttc gtgtagggcc agaggacgag
    15361 tgcgcgcgcc ggcagcaggc atgcgggatg cgggccgtaa ggctcctgcg ggatgatggc
    15421 ctgccccccg ccgcgcatga tggtgagcgc ccacgcggcc agctcgacgc cccacaaatt
    15481 ccggttgacg acgcggtgga gcaggcggac gccgctcccc tcggcgtcga gcgcgacggt
    15541 gatctccttc tgcatgcccg tcgcttgctc gggctgctgc cggaggcgga cgctcaactc
    15601 gtcgatgact tcaaattcga ccgcgtcgtt gtcgggcacg tagctgcggg gcatggcctc
    15661 gggcgccgtc cacaggcggt ggccgccgag cggcctccac tcgccgaggg gcgtcgcgac
    15721 ggacgcgccg ggcacttcgc cgagcacgtt ctcgccgccc gccagacagt agcggacgac
    15781 gcgcggcccc acgtcggtcg tgacgacgac ctccgcgtcc ccgttcgaca gccggtagca
    15841 gttcggcagg ttgaggtaag ttattttttc caactcgggt gatgtccgta gcggcggggc
    15901 gagtccgccg tctgctcaag ggatagaatg tttagacagg attgacagga cggacaggat
    15961 gaagaagagt tgatttcaga tttgagattt caaatttcag acttcaaatc tgttcgtcct
    16021 gtccgtcctg tcaatcctgt ccatttcctg ccttaatacg cgaacgactg gtaaatctcc
    16081 gtcccctcgt catcgacgga ttcgacgacc gggttgaagc gcaggagaaa gcgcgcgcgc
    16141 tccgagagcg ccgagagcgc gtcgcgcggg acgcgtatcg agacctcggg gcgaaacagc
    16201 cagcggcggc tgtagccgac caccaccatc gggcggggcc gcgccgtgcg gttcggcgtg
    16261 ccccggtgca ggccccgcac gtcgcgaatc atcacgtcgc cggcgcgcaa aagaatcggc
    16321 tcaagcgccg tctcgcccgc ctcgatgcgc cgcaggccct cctccttcga cagcaggtgc
    16381 gtgccccgcg ccacctcgaa ggggccgttc tcgggcgtca cgtcggcgag cgggaagttg
    16441 acggcgagct ggaagggcgg cgtctcgctg ccggtctcgg ggaaaagcgg cggcgcgtcg
    16501 cggtgcacgt cctgatagtc ggagcctaag agcggcgtgt cggtcgcgag ctggcacatc
    16561 acggcgtcct cgccgaccag tcccatgacc acgccgagca cgtcctcgtc ctcgaagacg
    16621 cgcgggtcac tgaacggctg gcggaagggc agcgtcacgt agtagcggag cggcccgcgg
    16681 ttctgcacgt ggccctcgcg ctccaggtgt ctctccagca acggcgcgaa agcctccccc
    16741 cacgcctcca gccgcgcgcg ctcgaagtgg ctcggcaaca cgcagaagcc gtcgcgcaaa
    16801 acctgcccga cgaacgactc gatctcgcta ctgcggtagc ggcctgtggt catagaaatt
    16861 tcgtcaatcg tcaattgtca atcgaagaga ctgtctctca cgattgacga ttgacgattg
    16921 acgattcacg gccttttcac ttcctcatgc tcagtcggtt ctgcacgatg acgacggcga
    16981 gcaggatgac gccgcgcagg acgagttgcc accacgggct gatctgccct tcgaggttaa
    17041 agacgttgaa gatcatgccg agcaggat
//
LOCUS       DASVES010000216        27875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_671912, whole genome shotgun sequence.
ACCESSION   DASVES010000216 DASVES010000000
VERSION     DASVES010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 27875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27875
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671912"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VF240_08620"
     CDS             <1..948
                     /locus_tag="VF240_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175306.1"
                     /translation="AVLCDALVFNRNDWTTTQAELDFALLDAGRDQLFYQPQYGALSS
                     FSTIANSIYHAGTLSIRERFGEKLYLDFNYTLSKSMDDASGLQTSGSYGAAFITNPLR
                     QRDNYSVSDFDIRHIINVNGVWQLPFGRGQRFLSDSGGVVQNVLGGWQLTGIMRWNSG
                     LPSSVPFDGATWATNWNLQSWGVRTRPLEACVASPNGGAPALFGCNLTEAYQSLRSAK
                     PGETGDRNVLRLPGYFVVDMGLGKSFEMPWSEKHKLQVRFEGFNITNTQRMGALVGGR
                     AGFGIVPDPFTAQPPPNWSAFGAIQGERRVMQFGFRYEF"
     gene            1095..2105
                     /locus_tag="VF240_08625"
     CDS             1095..2105
                     /locus_tag="VF240_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175307.1"
                     /translation="MSNTSARRDARAVLFAAPALLVASLFFCAPTARAQVTGGGHTLY
                     GDFSVDESRAKGMVPISFFIILYTETGLVVERQSVGINSRYRFINLRNGMYDVAVEVE
                     GREVARTRVNINSLYKNDFRQDLALAWSAPTGGPRPGTVDASAVYKRNSTNQDIFERA
                     DLAAGKKEYEEAAALFRRLVEADPADFPAWTELGTMNYAMGKSEEAEEAYARALKEKP
                     EFLRAAINLGRLRIAMKKYEPAIEVLSVAVGKNPQSPDANRLLGEAYLQARKGSKAVP
                     YLNEAARLGRADAHLRLATLYNNAGRKDLAAAEYEQFLTKEPKHADKEKLRRYIEENK
                     KQ"
     gene            2180..3175
                     /locus_tag="VF240_08630"
     CDS             2180..3175
                     /locus_tag="VF240_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX7175308.1"
                     /translation="MFIKSMLATLLWLALFCTGASAKETRFLTGNAADVKPKLHGPAY
                     HLQGGGTDVDAAFQWMIDRVRGCAGDCAVKLDVVVLRSSGGDGYNDYLFKMKGVDSVE
                     SLVIKERADSMSAAVRDTVRSAEIVFFAGGDQCNYVKYFKGTEVERSVERVHARGGGV
                     GGTSAGLAVMGDVAYDACSGGSAKSPESLANPYHADVTFTYDFFDWPVLRAVITDTHF
                     VERDRMGRLLVFVARQLKDGKYKSITGLAVERETSVVTDGRGAARVMGKGPVYLVVGD
                     HPPEVCEPNQPLTYSGYKLWKLPSGAVFDLKRRPRTGYYTVSVREGKILSDPYQK"
     gene            3288..4607
                     /locus_tag="VF240_08635"
     CDS             3288..4607
                     /locus_tag="VF240_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7175309.1"
                     /translation="MSERIYRDPVHNIIRLRTTTDEGRLMVQLVDCYEVQRLRRVKQL
                     GLALYTYQGAEHSRFSHSLGALHLMTRVLDRLGETCAIDEKDRAAARAAALLHDIGHG
                     PFSHVMEKVLGFHHEDLTVAAALSTETEVGQLLRDFSPDLPRRVADIIEGKFQPAYLA
                     QLVSSQLDVDRMDYLLRDSLMTGAKYGIYDLEWIVNALAVDEEADRIYVTARGIHAVE
                     EYLQARYYMFRQVYFHRTLRSAEAVMRSALRRALELTEQGREVYCAPGTPFEKVLHRL
                     PLALSDYMALDDSDVLFHIKQWQASGDPVLSDLCRRFTGRRLFKAIDLDMPEDARPDF
                     LAAARERTRERGFAPEYYFIEDHAGDVPYYSYYDAGGAEPKARIYVEDGYARPVMREI
                     TEVSDAVRGLRSYQLHRVCFPPEVKDEVYALYHGGRSQGSEARSQNV"
     gene            4667..5149
                     /locus_tag="VF240_08640"
     CDS             4667..5149
                     /locus_tag="VF240_08640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7175310.1"
                     /translation="MPDMLVNLLRLPPLEPMLEGARGAGVVVRRAQPWEVAPVRLFIV
                     ENFEPGWADEVAVGYANKPVSIYLALRERRVLGFAAYECTRRAFFGPMGVTVGERGHG
                     LGRALLVAGLHGLRELGYVYGIIGGAGPTEFYERAVGATVIPDSSPGIYEGSLQRADG
                     "
     gene            5182..6177
                     /locus_tag="VF240_08645"
     CDS             5182..6177
                     /locus_tag="VF240_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7175311.1"
                     /translation="MLLRFLLILSLICHAADGTRAAQHVVPRDDAARRPPRVAVLDFG
                     DTETGRRAAADVAAHLSKFAGLRLVDAAQARSAARGLGYTGSLNLTLEEARDLGAAID
                     CDYYLTGDAQTLRRSSSAVPVYHEAYASVFIVSARDGRLIAWQRPHAEAPTPEEAERL
                     LAAQLADHAEVFRISMFRDEEDERARRRRESLSSPADDPPYFEDAPEEGSDASKDFVP
                     PHPYQRVRPAYPDTAAQAEAEATVDAAVEIDERGEVIGVRVVRWGGFGLDHAVVETLR
                     KMHFRPATRSGRAVPVRVLMRYNFRRPARPGQPQQAGEGEMKLGPAFKALLNRTP"
     gene            6234..7667
                     /locus_tag="VF240_08650"
     CDS             6234..7667
                     /locus_tag="VF240_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7175312.1"
                     /translation="MPHRASNILRVVLLLCCALALLSPTPAASAPDDRAAARRPRHVT
                     AETREGRLRIFDEVWETVRALYYDPNLHGLDWDGLRAKYRPEAASAGGPQELYSVLRR
                     MLAHLSDPHTRVYEPGRGADWRETRHIAVGVQLRELSGEPVVARVERGSPAERAGVRA
                     GDILLSIDAAPVHQLLARRSDAEQMADAQPSDTQPADDGRAFARASVKRRARRVAGLF
                     DGPESSTFEAVFREEGGRERTVRLTRELVARTPALRVRRASGAYVVVEFNLFTQEIAA
                     GVARVFRDELKDARGLILDLRDNGGGEADAMTDIASLLLPPGLPLGRFDDRAGRTRLE
                     PRTRAALLSSADAPARFSGPVVVLTGARTASAAEVFAAALRESGRAARVVGEETCGCV
                     LGIRRRHTLPDGGTLDVSEMDYRTAAGQRLEGSGVEPDELVTPTRRSLREGKDPALTR
                     AVEILKEAGRKRPSAQSSMSRGSGGRM"
     gene            complement(7889..8224)
                     /locus_tag="VF240_08655"
     CDS             complement(7889..8224)
                     /locus_tag="VF240_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175313.1"
                     /translation="MPRTCEYCQSTLEDGAKKCADCGAPPTAAQPTDYRICPFCRRRL
                     LALGSPACNYCGRALPEDYIKAREATLRRIGEAQTAEEREELEQDSDDALKRALKSLF
                     DLDETARRK"
     gene            complement(8255..8890)
                     /locus_tag="VF240_08660"
     CDS             complement(8255..8890)
                     /locus_tag="VF240_08660"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX7175314.1"
                     /translation="MLLAFVLAGCDYVARQTDRVAGSGNKKMESREVPEFDEIVVNGA
                     YKVEVTCGQPRSVQLEADDNILPLIKTVVEGGRLTITQERGMSINSAPVVRIGVPDLK
                     LVSIPGASDFRLSGVRNEALKISVEGAARLHAAGETGTLDLSLNGAGLIDARELRARR
                     ATVVSNGAGLTSVHATEALDATVNGIGGIDYYGDPKAVAPQINGLGRINKK"
     gene            complement(9062..10219)
                     /locus_tag="VF240_08665"
     CDS             complement(9062..10219)
                     /locus_tag="VF240_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7175315.1"
                     /translation="MALEILQYFDPTGAAVVHRIPESGATDIKMGAQLIVQENQSAVF
                     YRDGKALDTFGPGRHTLTTMNVPLLTRLLSIPFGGTSPFQAAVVFVARHTFQDMKWGT
                     KEPIPFRDSELFMVRLRAFGKYSFRVADPQLFVGSIVGTRGIVSTNSIADFLRDIVVS
                     RLNDLLGENLKTVLDLPAYYDELAAGVKARVAEDFSKQGLELVDMLISAITPPEEVQK
                     KIDERASMGALGDMDKYMRYKAAESMQDAAKQPGGAAGQGMGLGMGFGFGQMMAGAIG
                     GGQGQQQGGGQQGQGGGGAGMAAAQGSPCPKCGTVNAAGVKFCSNCGEKTQQQQQAAT
                     VACPKCDAQVAAGAKFCSSCGQSMEPKKCQNCEAPLSAGAKFCGGCGTQQT"
     gene            complement(10316..11482)
                     /locus_tag="VF240_08670"
     CDS             complement(10316..11482)
                     /locus_tag="VF240_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7175316.1"
                     /translation="MTERCTNCGTELFTGQQFCRRCGAPTAPLAGEAPTQILRDTAAP
                     PPPSASATTTLPPQRGTGAGLWGPRHTGPQTPFPSFAHTSPLASAPAAAQPSKGRRWL
                     IPALLGLLLIAVLGVSLGVVVFRGAQRRMAAVERGMPPPAPPAPPGLPGLAPGESVLD
                     EAGAIVTADETVLTKTFELGDDAVFTLKNTQGDIVVEGWDEDRVELKVTKRGGSAEDR
                     QAARVRLTQDDELLALSSADNRQVKISYEVKLPRSLERMELSSRSGDVQVSKFTGSLG
                     VTLQNGDIALDDVRGPIEAKLVNGDIEVVYRDAEREGPHEFINVNGDVTLRLIEGMDA
                     DLKATIVNGSIDVDNQLGFQAQKRQPGWHVDTRLGDGGAPLTVKNVNGSIRFKK"
     gene            complement(11579..12145)
                     /locus_tag="VF240_08675"
     CDS             complement(11579..12145)
                     /locus_tag="VF240_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175317.1"
                     /translation="MNDQRNWLERLGEKIPGYGGYVQKERRRDIDKLHREHLADRLRT
                     LKQTLNDVVRELTDGRRLMEVGPVDRAAKKVDMVENRVRYASYGYGGLFDVVKIEEPQ
                     LDRLYQFDLALVQKVEELEQHARALKDGAGTAEGLKGAAANLERALDEFNRAFDERSR
                     SINDTGGGGGSPEDGPQGGRPGPMFGVQ"
     gene            12551..13372
                     /locus_tag="VF240_08680"
     CDS             12551..13372
                     /locus_tag="VF240_08680"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEX7175318.1"
                     /translation="MSEETDQHTSQAPTPEGKYVYCIIETKEPRNFGPIGIGGRGDDI
                     YTVHHDGLAAVVSNTLLQVYDPTRENVFAHEQVNETVMREFTVLPMAFGVLFRTEEDI
                     IELMRGTYDALRDVLAKMEGKVEFGLKVNWDRERVMAELEQESDEIRNLKEQITSRAT
                     GSTYFARMQLGRLIENALNDKSEGYVHEVYAVLRDTAVASRANKPIGDRMIMNAAFLV
                     EREREPEFDAHVKQVAAKYEDKLSFKYTGPWPPYNFVHIRLKLERGEGAADAIQK"
     gene            13671..14006
                     /locus_tag="VF240_08685"
     CDS             13671..14006
                     /locus_tag="VF240_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HEX7175319.1"
                     /translation="MFFIDDILLAPVNGFKFILRQIEELANRELNDDTAIKEQLLELQ
                     MRLELGEVSDEEYVEAERELFARLRVIKERQLAALSQVHTAESSTMTVEAGGDEGGEF
                     WEPGGGSER"
     gene            14003..16141
                     /locus_tag="VF240_08690"
     CDS             14003..16141
                     /locus_tag="VF240_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX7175320.1"
                     /translation="MTRRERAGGGGARAAPRPARKSSQKKSSKSPRRKASAPDASLAP
                     ATDFPAARFLFFGGKGGVGKTTAACAAALLLLERAAAGERLLLFSTDPAHSLSDSLDT
                     SVGDRLIEVTPRALRRTAANAPRLFAREMDAAAALEEFKTRHRAVLKEIADRGTLLDE
                     ADINDLLDLSLPGMDEVMSLFELSELARGGEYARVVVDTAPSGHTSRLLRLPETFARW
                     TLALDRMNEKHRYLLAHFVRGRRFAADDVELFLRELTERVAAVRATLFDAAGASFTLV
                     TIPEAMSVEESARYYRLLKDEGVPVTDLIVNRVEREHDACPYCRARARAQSPWLAEIE
                     REFKDLHIRRVPLLPEEVRGDVRLRRFAALAWGTGGSPTVREGVKATPPSARPAKKRP
                     PSRSGFRLGEKRLVIFGGKGGVGKTTAAASAALALAESDPARRVLVFSTDPAHSLSDS
                     FDEEVGALKEGVAGVGNLDAMEIDPAVRFEELKDRYRRWTDELFDSLTGGTRWEVKFD
                     REAMREMVSLAPPGIDEIAALSAVSDLIDADTYQTIVLDTAPTGHLLRFLELPEVALS
                     WVRTFIKLMLKYKGLVRYGSVAEELVALSKSIKRVSALLGDAGECEFVGVAIPETMSL
                     EETARLAESLARLGVPMRRLLVNNVVSAEAALACDFCAARHAAQAPVLKNFAERFDGA
                     VELFLAPQQPHDIHGPALLHEHFEQWRSAS"
     gene            16178..17032
                     /locus_tag="VF240_08695"
     CDS             16178..17032
                     /locus_tag="VF240_08695"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEX7175321.1"
                     /translation="MSDSATKKNAGGDGPEEAAALYVYCVGERAELEPLLSDEGVPAA
                     IEESGALETVGAGALAAVVSAVPLADYGEEALEARMRDATWTATRAMRHERAVEFFAR
                     RASVVPLRFGTIYLTRARVAAMLDERDAELRSAAGRLRGREEWGVNVYFDRARLKEMV
                     VELSPRLRALAGQASAAAPGQSYLLRKKIEAMRADEARAEVRRVAGEVETKLAATAAS
                     AARLRVLKDEASEHGDVAAKLAFLVERERFPEFRDAAEALAQKYEPAGFRLELTGPWP
                     AYNFAAGE"
     gene            17142..17813
                     /gene="gvpJ"
                     /locus_tag="VF240_08700"
     CDS             17142..17813
                     /gene="gvpJ"
                     /locus_tag="VF240_08700"
                     /inference="COORDINATES: protein motif:HMM:NF016977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HEX7175322.1"
                     /translation="MTEELEFTGFDEDEELSLLETLNHVLDKGVVVAGDITISVANID
                     LIFVNLNALLGSVETVNEVLSRRRDPAPAPALAARAHDEGAKADEKKVDTRRESGVLL
                     TEQFPLPPEPLESEVSDARAGAAVEQLRRETGRLPERINIDPRSVEQGLAKLVLTLIE
                     LIRRLLEKQAVRRMEGGRLNDAQVEELGLALMKLEEKMRELKSQFGLEDEDLNLDLGP
                     IGRLI"
     gene            17915..18328
                     /locus_tag="VF240_08705"
     CDS             17915..18328
                     /locus_tag="VF240_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HEX7175323.1"
                     /translation="MSSERTSLISARPPGLVDVLDRILDKGLVIAGDVKVSLANVELL
                     TLQIRLLICSIDKAEQIGLNWWRYDSNLTTRAERAETENLELRERLAALEREIRRLDA
                     HAPAAPVAQTAAGPLKRAASKPAGPPSRKGRNRSP"
     gene            18468..18785
                     /gene="gvpA"
                     /locus_tag="VF240_08710"
     CDS             18468..18785
                     /gene="gvpA"
                     /locus_tag="VF240_08710"
                     /inference="COORDINATES: protein motif:HMM:NF006874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_component: GO:0033172 - gas vesicle shell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle structural protein GvpA"
                     /protein_id="HEX7175324.1"
                     /translation="MAVERAPGGTSLIDVLDRVLDKGIVIDAWVRISLVGIDLITVEA
                     RVVVASIDTYLKYAEAVGLTPAVSRPAMGPAPAAAIGPAPVAAEPIGEETRRRTTIEV
                     ERQ"
     gene            18882..21086
                     /locus_tag="VF240_08715"
     CDS             18882..21086
                     /locus_tag="VF240_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7175325.1"
                     /translation="MDRLTETSQAEPAAAGVAGGDARARTPETERHYELLFETTTQLL
                     AAPTVEERMLLTLDAVTSGLGYPHAAVALIDKRQVTLKVRMAVGFVDDAAVCDVETPL
                     DANGPHVSVVHDGHPAWVTRRAGEPASVAFLDRLGCRDTDLLALPLFGGQPGVAEKTG
                     GEFARRPYDDERYWMPEAMCMGALYVGALQVEMGEEQYRLLTRLADRVGVILSAAVQM
                     EQLNSSVRKLQRERQWVESVMQSVADPIVLTNLDNEILLQNRRAEQLFSGQKNASPGK
                     RRALEMNDLLFSAYLSSAAVASEGIVARDLTLVDPIDGSDIHFEVVSTPALDTRAERI
                     GLVSVFRDVTDLRHANEELARNFAKLQQAEQEARRERDRLDLILENVGHPVAVSDPAG
                     NFILFNSRAERLFQEQADMAPRAARAARANAVKLTSFISGLASEAGSGRRDEIALIDP
                     ELGESLPMEITAAEVTDARGQVTAVVSILHDLTEIRELERRRVEQQLFESEKLAAVGR
                     LAASIAHEVNNPLEAIKNSLYLLTTSQNQDSNTRFLEVARKETERVSHIIRQMLGFAR
                     RSGEVEPTDVNQIIEETLVLVEKKMKQARVRVARELEAGLPQVRARADQLRQVFLNLI
                     LNAQQSIDGEGEVRIQTGRYDQALQPSISVRISDTGKGISEQDVARIFEPFFSTRAKG
                     TGLGLWVSQDIVRHHGGRIEVTSIEGQGTTFNIILPIDSPTLTETDVSPKKH"
     gene            21064..21945
                     /locus_tag="VF240_08720"
     CDS             21064..21945
                     /locus_tag="VF240_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175326.1"
                     /translation="MSAQRSTNGDGTTGALGGSGRRAPTRKRVLVVDDEENVRVTTAA
                     ILEQDGYYVDTAGDGREALQKVERGGFDLVLTDLRMDDMDGAALLNELHTRHPSVVTV
                     VLTGYASIESSIDALRQGVYDYLVKPCVIEDLKRTVRRALEHRDQKARISELSNPVVE
                     IWDGILLLPLVGTLDDQRSSQMSTTLLNAVRAKSAQAVIIDITGCTVVDTYTVAHLIN
                     TVRSVHLLGATGVITGVGASMASDLVKLGIDLHEINTRRRLADGLRFAFELLNVGIRG
                     QRSEVGGQKKRDLFTDL"
     gene            21973..23613
                     /locus_tag="VF240_08725"
     CDS             21973..23613
                     /locus_tag="VF240_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175327.1"
                     /translation="MSSLDPTQTNGHEATRLLVVDDEESVAITVSEVLRLEGFEVDTA
                     MSGADAAVQLGRAHYDLVLTDLHMEEGDGISVLEEVRRVSPMTISIVLTGFASVESAI
                     AALRKGAYDYLVKPCIIEELKLTVRRGLEHRRLMLAEQSARLGLQDLNRELEQRVLDR
                     TAELQRLNDELLEANRAKDVFLATLSHELRTPLTPVLGWVNLLRATGASGDPNLLAQG
                     LDAIDRNARLQARLIDDLLDISRIISGKLRLEWEVVDLCQVVEAAAETVRGSAEEKGV
                     RLEVQTSAAPLVVRGASVRLQQIVWNLLANAVKFTPPAGVVSLRLAREGREARVVVED
                     TGSGIAPEFLPHVFDRFRQADGSTTRQHGGLGLGLAIVRALADLHGGRVEAESEGPGH
                     GSRFIFALPCAASAAVIDEAMASVDAPTLARPVLVVDDSPETLELLEMFFSRKGYEVL
                     TADSAERALELARAEPPALVISDISMPNVDGYQLLARLRELPGLGDVPAIALSGYAME
                     EDRQHALEAGFAVHIAKPVDPDELLATIQQLTSSEEQG"
     gene            24242..26917
                     /locus_tag="VF240_08730"
     CDS             24242..26917
                     /locus_tag="VF240_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175328.1"
                     /translation="MTTKSNRKAGGGKRAGKSAAGAKKASGKTAARKKAGKRKQAVER
                     EEGPLGWLVPTLEASYTRLVPREEYEAAQAEAARDAAAKKAAGKKAGAKKAGAKKAGA
                     RKAGSKKSGAAGPEAFESTLQPGEGESVLADVADNLWVDRLAEYKRRKAASALGLGGA
                     PAAVASGAARAGAAPLPPGPVVPGQKNWGPLGPSVVMEAQAHGLPAVGGRVSGIAIAP
                     GGQLVYAATANGGVFRSEDAGISWRSLMDAFDVDPTTFASTSLACGAIAIDRNDPKRI
                     YVGTGEGDTHGMFQQRIINALPAYRGIGPIRSDDGGQNWVEEPTASGSPTLAGKAFFA
                     LAVDPANRESVVGATTDGLYQRTLATGGAPSWTRRRAGLFSSVVVATSGSTKRFFAAE
                     WGRGVIRSSNGSSWSTLGAGFPTTNVGRIALAVQPNNPNLVYAFVADANGASRGVFRL
                     NSVTGAWKQVTGVPSVLPSPQGDYDLAIAVDPTDADLIYLGGSYFHDNEFWPASVWRC
                     RVKAVGSGFTMSGVSIGKHAHADVHVLTHSPNDPNALWVGCDGGVFLNRDPRGTGIFA
                     SRNNGLSCLCTNFFAQHPTDPNIIFCGLQDNGTARTGGGPIWRHVNWGDGGYCLINWA
                     DPRQVLVFANGSIYRATDGGLSHNSWSRTEFDWATMTEPIVGPPFNPSKPAEAKLVAL
                     GAGRQVRLSKDFGASWLAADIVSLPGADPSGSIFSLSFASASRFFVGTTAGDVFRVDK
                     SGSSWTATRIDNAGAGPLPLRGLISDIAVDWADPTLSSIYVAFGGMGDFRHVWRFNGT
                     RWQARSGAATGSTNLLDVEHNAITVDKSAPANVYVGADIGVWHSRDGGLNWVTLPNGL
                     PDAPIFDLQTHPTRRILRAATHGRGIYEYPLDPAS"
     gene            26988..27563
                     /locus_tag="VF240_08735"
     CDS             26988..27563
                     /locus_tag="VF240_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX7175329.1"
                     /translation="MLQTLATAYVLVLVAELVGDKLIYTAGSLTTRFRALPVFCGLSL
                     AFMLKMLAAVSVGRFIAELPGAVVAGVSAATFFAAAAVIWFRRGGGEASPKAEAEAGW
                     WRAVGVSFAAAFLTEWGDIGQLTAATLAARHNAPLVVWFGATLALMTKGALAVTLGVG
                     LRRKVPARVLRPLAASVCVVMGLVSAHGVFR"
     gene            complement(27647..>27875)
                     /locus_tag="VF240_08740"
     CDS             complement(27647..>27875)
                     /locus_tag="VF240_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7175330.1"
                     /translation="QIGVAYTLFTRGIERGVRSLDAGLVGYVEPVSNPVWVYLFIGER
                     PSPWAIAGGAVIIAAVVAHTVLSARTRQKRP"
BASE COUNT         4693 a         9778 c         8916 g         4488 t
ORIGIN      
        1 gccgtcctct gcgacgccct cgtcttcaac cgcaacgact ggacgacgac gcaggccgaa
       61 ctcgacttcg ccctgctcga cgcgggccgc gaccagctct tctaccagcc gcagtacggc
      121 gcgctctcgt ccttctccac catcgccaac tccatctacc acgccggcac gctctcgatc
      181 cgcgagcgct tcggcgagaa gctctacctc gacttcaact acacgctgtc gaaatcgatg
      241 gacgacgcct ccggccttca gacctccggc agctacggcg cggccttcat caccaaccct
      301 ctgcggcagc gcgacaacta ctcggtctcc gacttcgaca tccgccacat catcaacgtc
      361 aacggcgtgt ggcaactccc gttcgggcgg ggccagcggt tcctgagcga ctcaggtgga
      421 gtcgtccaga acgtcctcgg cggctggcag ctcaccggca tcatgcgctg gaactcgggg
      481 ctcccgtcct ccgtgccgtt cgacggcgcc acctgggcga ccaactggaa cctccagtcg
      541 tggggcgtga ggacgcgccc gctggaggcg tgcgtcgcca gccccaacgg gggggcgccc
      601 gcgctcttcg gctgcaatct caccgaggcc taccagagcc tgcgcagcgc caagcccgga
      661 gagaccggag accgcaacgt cctcagactc cccggctact tcgtcgtgga catgggtctc
      721 ggcaagtcgt tcgagatgcc ctggagcgag aagcacaaac tccaggtgcg cttcgagggc
      781 ttcaacatca cgaacacaca gcggatgggc gcgctcgtcg gcggacgcgc cggcttcggc
      841 attgtgcccg atccgttcac cgcgcagccg ccgcccaact ggtcggcctt cggcgcgatt
      901 cagggcgagc ggcgcgtcat gcagttcggg ttccgctacg agttctagcg ggcggccccg
      961 gataaaccaa acgcccactc tcaagacgcg ggccggggtc gccagcctcg gcccgctttt
     1021 tttcagtcag cgcggcgcgc gacgaccgac gcacgcgccg gtcgtcgcgc atccgaagac
     1081 ccggaggagc aatcatgtca aacacgtccg cccgccggga cgcccgcgcc gtcctgttcg
     1141 ccgccccggc tctgctcgtg gcctccctgt tcttctgcgc ccccacggcg cgcgcgcagg
     1201 tgaccggcgg cggccacacc ctctacggcg atttcagtgt ggacgagagc cgcgccaaag
     1261 ggatggtgcc tatctccttc ttcatcatcc tctacacgga gaccggactc gtcgtcgagc
     1321 gccagtcggt gggcatcaac agccgatacc gcttcatcaa tctgcgtaac ggcatgtacg
     1381 acgtcgccgt cgaggtcgag ggccgcgagg tggcgcgcac gcgcgtcaac atcaactcgc
     1441 tttacaagaa cgactttcgt caagacctcg cgctcgcctg gagcgccccg accggcggcc
     1501 ctcgccccgg caccgttgac gcctccgccg tctacaagcg caactcgacc aatcaggaca
     1561 tcttcgagcg cgccgacctt gccgccggga agaaggagta cgaagaggcc gccgcgctct
     1621 tccgcaggct cgtcgaggcc gaccccgccg acttccccgc gtggacggag ctggggacga
     1681 tgaactatgc gatgggcaag tccgaggagg ccgaagaagc atacgcgcgc gcgctcaagg
     1741 agaagcccga attcttacgc gccgccatca acctcggacg cctgcgcatc gccatgaaga
     1801 agtacgagcc ggccatcgag gtgctcagcg tggcggtcgg gaagaatccg cagtcgccgg
     1861 acgccaatcg tttgctcggc gaagcctacc tgcaagccag gaaaggctcg aaggccgtcc
     1921 cctatctgaa cgaagccgcg cgtctgggcc gcgccgacgc gcacctgcgg ctcgccacgc
     1981 tctacaacaa cgccggccgg aaagacctcg ccgccgccga gtacgaacag ttcctcacca
     2041 aagagccgaa acacgccgac aaggagaaac tgcggcgcta catcgaagag aataaaaagc
     2101 agtagcctcg accggcggcc gccccgcacc cgcctgacga acattcggga gggattcctt
     2161 gctcggacac gcgtcttcat tgttcatcaa atccatgctc gcgacgcttt tgtggctcgc
     2221 gctcttctgc acgggggctt cggccaaaga gacgcgcttc ctgaccggga atgccgccga
     2281 cgtgaagccg aaacttcacg gccccgcata tcatttgcag ggcggcggca cggacgttga
     2341 cgccgccttt caatggatga tcgaccgggt gcggggctgc gccggcgatt gcgccgtcaa
     2401 gctcgacgtg gtcgtcctgc gctcgtccgg cggcgacggc tacaacgact acctcttcaa
     2461 gatgaagggc gtcgattccg tcgagtcgct cgtcatcaag gagcgcgccg actcgatgag
     2521 cgcggccgtg cgcgacaccg tgcgtagcgc cgagatcgtc tttttcgcgg gcggcgacca
     2581 gtgcaactac gtcaagtatt tcaagggcac ggaggttgag cgaagtgtcg agcgggtaca
     2641 cgcgcgcggc gggggcgtcg gcggcacgag cgccggcctc gccgtcatgg gcgacgtggc
     2701 ctacgacgcc tgctccggcg gcagcgccaa gtccccggag tctctggcga acccgtacca
     2761 cgccgacgtg accttcacct acgacttctt cgactggccg gtgctccgcg cggtcatcac
     2821 cgacacgcat ttcgtcgagc gcgaccgcat ggggcggctg ctcgtcttcg tcgcgcgaca
     2881 gctcaaagac ggcaagtata agagcatcac gggcctcgcc gtcgagcgcg agacctccgt
     2941 ggtgacggac ggccgaggcg cggcgcgcgt catgggcaag gggccggtct acctcgtcgt
     3001 cggcgaccac ccgcccgagg tgtgtgagcc taaccagccg ctgacttact cgggctacaa
     3061 actctggaag ctcccgagcg gcgcggtctt cgacctcaag cgccggcccc gaaccggcta
     3121 ctacacggtc agcgtgcgcg aggggaaaat tctctccgac ccatatcaaa agtaagcagt
     3181 gagcagtaag cagtaagcag tcaagacaag ttatgccttc tggctgctca ctgcttactg
     3241 ctcactgctt actctcttca tccctccgtt gtgatagact ttcgcctgtg agcgagcgca
     3301 tctaccgcga ccccgtacac aacatcatcc gcctgcgcac cacgacggac gagggccggc
     3361 tgatggtgca gctcgtggac tgttacgagg tccagcgcct gcggcgcgtc aagcagttgg
     3421 gcctcgcgct ctacacctat cagggcgccg agcactcccg cttctcccac tccctcggcg
     3481 cgcttcacct gatgacgcgc gtcctcgacc gcctcggcga gacctgcgcg atagatgaga
     3541 aagaccgcgc cgccgcccgc gccgccgccc tcctccacga catcggccac ggccccttct
     3601 cacacgtcat ggagaaggtc ttaggcttcc accacgaaga cctcaccgtc gccgccgcgc
     3661 tgagcacgga gacggaggtc ggccaactcc tccgcgactt ctcgcctgac ttgccgcgcc
     3721 gcgtggccga catcatcgag ggcaagttcc agcccgccta cctcgcgcaa ctcgtctctt
     3781 cgcagctcga cgtagaccgc atggactatc tgctgcgcga ctccctgatg accggcgcca
     3841 agtacggcat ctacgatctt gaatggatcg ttaacgcctt ggccgtggac gaggaagccg
     3901 accgcatcta cgtcacggcg cgcggcatcc acgccgtcga ggagtacttg caggcccgct
     3961 actacatgtt ccggcaggtc tacttccacc gcacactgcg ctcggccgag gccgtcatgc
     4021 gctcggccct gcggcgcgcg ctcgaactca cggagcaggg gcgcgaggtc tactgcgcgc
     4081 cgggcacgcc cttcgagaag gtgctgcacc gtctgccgct ggcgctctcg gactacatgg
     4141 cgctcgacga ctccgacgtg ctcttccaca tcaagcagtg gcaggcgtcc ggcgacccgg
     4201 tcctctccga cctctgccgc cgcttcaccg gccgccggct cttcaaggcc atcgacctcg
     4261 acatgcccga ggatgcgcgc cccgacttcc tcgccgccgc gcgcgaacgc acgcgcgagc
     4321 ggggcttcgc ccccgagtac tatttcatcg aagaccacgc cggcgacgtg ccctactatt
     4381 cctattacga cgcgggcggg gccgagccga aggcgcgcat ctatgtcgag gacggttacg
     4441 cccgccccgt catgcgcgag atcaccgagg tgagcgacgc cgtgcggggg ctgcgtagtt
     4501 atcaactgca ccgcgtctgc ttcccgcccg aggtcaagga cgaggtctac gcgctctacc
     4561 acggaggaag gagtcagggg tcagaagcca gaagtcagaa tgtgtgacag ccggagagtt
     4621 ctttattctg tcttctgact cctgactgct gactcccctg ttttttatgc ccgacatgct
     4681 cgtcaacttg ctccggctgc cgccgttgga gccgatgctc gaaggggcgc ggggcgccgg
     4741 cgtcgtcgtg cggcgcgcac agccgtggga ggtcgcgccc gtgcgcctct ttatcgtgga
     4801 gaacttcgag ccggggtggg ccgacgaggt ggccgtcggc tacgccaaca agcccgtctc
     4861 tatttacctc gcgctgcgcg agcggcgcgt gctggggttt gcggcctacg aatgtacgcg
     4921 ccgcgctttc ttcggcccga tgggcgtgac cgtcggcgag cgcgggcatg gactcgggcg
     4981 cgcgctcctc gtcgccggtc ttcacggcct gcgcgagttg ggctacgtct acggcatcat
     5041 cggcggcgcc gggccgaccg agttttacga gcgcgccgtc ggcgccaccg tcatccccga
     5101 cagctcgccc ggcatctacg aaggctcgct acagcgcgcc gatggttaga tttttgcgac
     5161 cgcgccgcga ccgcgtccct catgcttctg cgcttccttc tcatcctttc gctcatctgc
     5221 cacgcggcgg acggcactcg cgccgcgcag cacgttgtgc cgcgcgacga cgcagcgcgc
     5281 cggcccccgc gcgtcgccgt gctcgacttc ggcgacacgg agacggggcg gcgagcggca
     5341 gccgacgtgg cggcgcatct gtcgaagttt gcgggcttgc gtctcgtcga tgcggcgcag
     5401 gcgcgctccg ccgcccgcgg cctcggctac acggggtcgc tcaatctcac gctcgaagag
     5461 gctcgcgacc tcggcgcggc aatcgactgc gactactacc tcacgggcga cgcgcagacc
     5521 ctccgccgct cgtcttcggc tgtgcccgtc taccacgagg catatgcctc cgtcttcatc
     5581 gtcagcgccc gcgacgggcg tctcatcgcg tggcagcgcc cgcacgccga agcgcccacg
     5641 cccgaggaag ccgagcggct gctggccgca cagttggccg accacgccga agtctttcgc
     5701 atcagtatgt ttcgcgacga ggaagacgag cgggcgcgcc gccgacgcga gtcgttgtcg
     5761 tcgccggcgg acgacccgcc gtattttgag gacgcacccg aagagggcag cgacgcgtcg
     5821 aaagacttcg tgccgccgca cccgtaccag cgcgtgcgtc ccgcctaccc cgacaccgcc
     5881 gcgcaggccg aggccgaggc gaccgtggac gccgccgtcg agatcgacga gcgcggcgag
     5941 gtcatcggcg tccgcgtggt gcgctggggc ggcttcggcc tcgaccacgc ggtcgtcgag
     6001 acacttcgga agatgcactt ccgcccggcc acgcgctcag gccgcgccgt ccccgtccgc
     6061 gtgctcatgc gctacaactt ccgccgcccc gcgcggcccg gccagccgca gcaggcgggc
     6121 gagggcgaga tgaaactcgg ccccgccttc aaggcgcttc tcaaccgaac gccctgagac
     6181 ggcgcgcccg cgcgaatctg ttagagaccg ccctgtcgta aatcctttag caaatgccgc
     6241 atcgagcctc aaacattctc cgcgtcgtct tactgctctg ctgcgcgctc gcgctgctgt
     6301 cgccgacgcc cgccgcgtcc gcgccggacg accgtgcggc ggctcggcgg ccgcgacacg
     6361 tcacggcgga gacgcgcgag ggccggctga gaatcttcga cgaagtttgg gagacggtgc
     6421 gcgccctcta ctacgacccg aacctgcacg ggcttgactg ggacggcctg cgcgcgaagt
     6481 accgccccga ggccgcgagc gccggcggcc cacaggagct ttactccgtg ctccggcgca
     6541 tgctcgcgca cctctccgac ccgcacacgc gcgtctacga gccgggccgc ggcgcggact
     6601 ggcgcgagac gcgacacatc gccgtcggcg tccagctccg cgagctgtcg ggcgaaccag
     6661 tcgtcgcgcg cgtggaacgc ggctcgccgg ccgagcgcgc gggcgtgcgc gcgggcgaca
     6721 tactcctcag catcgacgcc gcgcccgtcc accaactcct cgcgcgccga agcgacgcgg
     6781 aacagatggc cgacgcgcag ccatcagata cgcagccagc cgacgacggg cgcgcgtttg
     6841 cccgcgcgtc tgtgaaaagg cgtgcgcgtc gtgtcgcggg tctcttcgat gggccggaga
     6901 gttcgacctt cgaggccgtc ttccgcgaag agggcgggcg cgagcgcacc gtccgcctga
     6961 cgcgcgaact cgtcgcacgc acgcccgcgc tccgcgtccg gcgcgcgtcc ggcgcgtatg
     7021 tggtagtcga gttcaacctc ttcacacagg agatcgccgc cggggtggcg cgcgtcttca
     7081 gggacgagtt gaaggatgcg cgcgggctga ttctggattt gcgcgacaac ggcggcggcg
     7141 aggccgacgc gatgactgac atcgcctccc tccttctccc gcccggcctt ccgctcgggc
     7201 gcttcgacga ccgcgccggc cgcacgcgtc tcgaaccacg cacgcgcgcc gcgctcctct
     7261 cgtcggccga cgcgcccgcg cgcttctcgg ggccggtcgt cgtcctcacg ggcgcacgca
     7321 cggcgagcgc cgccgaagtc tttgccgccg ccctgcgcga gtcgggtcgc gccgcgcgcg
     7381 tcgtcggcga ggagacgtgc ggctgcgtgc tcggcatccg ccgccgccac acgctccccg
     7441 acggcggcac gctcgacgtg agcgaaatgg actaccgcac ggccgccggc caacgcctcg
     7501 aaggctccgg cgtcgagcct gacgaactcg tcacgcccac acgccgcagc ctccgcgaag
     7561 gaaaagaccc ggccctcaca cgcgccgtcg agattcttaa agaggcggga aggaagcggc
     7621 cgagcgcaca aagttcaatg agccgcggca gcggcggaag aatgtagccc cggtcgcgag
     7681 accggggtat gcgcccggca taaacgaagg aagccccgga ggggcgaaag aatctcgacg
     7741 atgtttattc tcaacgtcga gattctttcg cccctccgag gcttcgactt tcttcggcgt
     7801 cttccccacg gcttacgccg tgggctacat tcttccgccg ctaacgcggc tggttgactc
     7861 gcttccttcg gctcgtgttc tcaagagact atttgcggcg tgcggtttcg tcgaggtcga
     7921 agagtgattt gagggcgcgc ttcagggcgt cgtccgaatc ctgctccagt tcctccctct
     7981 cctccgcggt ctgcgcctcg ccgatgcgcc gcagggtcgc ctcgcgcgcc ttgatgtaat
     8041 cctccgggag cgcgcgcccg cagtagttgc aggcggggct gcccaacgcg agcaggcggc
     8101 gacgacagaa ggggcagatg cggtagtcgg taggctgggc ggcggtgggc ggcgcgccgc
     8161 agtcggcgca cttcttcgcg ccgtcttcga gtgtgctctg gcagtattca cacgtccgtg
     8221 gcatcgtctc gccgagccgg catggcgcgt ccgtctactt tttgttgatt ctcccgaggc
     8281 cgttgatctg cggggcgacg gccttcgggt cgccgtagta gtcgatgccg ccgatgccgt
     8341 tgaccgtggc gtcgagcgcc tctgtggcgt ggacggaggt caggcccgcg ccgttggaga
     8401 cgaccgtggc gcggcgggcg cgcagctcgc gcgcgtcgat gaggcccgcg ccgttcaagc
     8461 tgaggtcgag cgtgccggtc tcgcccgcgg cgtgcaggcg ggccgcgccc tccacgctga
     8521 tcttcagggc ctcgttacgc acgccgctca gacggaaatc gctcgcgccg ggaatcgaca
     8581 ccagtttcaa gtcgggcacg ccgatgcgga cgaccggcgc cgaattgatg ctcatgccgc
     8641 gctcctgcgt gatcgtcaag cgcccgccct cgaccacggt tttgatgagc ggcaggatgt
     8701 tgtcgtcggc ttcgagttga accgagcgcg gctgcccgca tgtgacttcg accttgtacg
     8761 ccccgttgac gacgatctcg tcgaactccg gcacctctcg actctccatc ttcttattgc
     8821 ccgagccggc gaccctgtcg gtctgccgcg ccacgtagtc gcagccggcg agcacgaagg
     8881 cgagcagcac gcaggcgatt gcgaaaaagg gatgaaggcg gagggatgag ggatgaacaa
     8941 aggcaggagg cagtaagcag gaagcaggtg gcagtcgagg acgctcggcg tctgcccact
     9001 gcctcctgcc caccgctgac ggcctactcg ccttcatccc tcatccctcc gccctcatcc
     9061 cttatgtctg ctgcgtgccg caaccgccgc agaacttcgc gccggccgag agcggcgctt
     9121 cgcagttctg gcacttcttc ggctccatcg attgcccgca gctcgaacag aacttcgcgc
     9181 cggccgcgac ctgcgcgtcg cacttcgggc acgcgaccgt cgcggcctgc tgctgctgct
     9241 gcgtcttctc gccgcagttc gagcagaact tcacgcccgc cgcgttcacg gttccgcact
     9301 tcgggcacgg gctgccctgc gccgccgcca tccccgcgcc gccgccgcct tgtccctgct
     9361 gccctccgcc ctgctgctgg ccctgcccgc cgccgatggc gcccgccatc atctgcccga
     9421 agccgaagcc catgccgagg cccatgccct gcccggccgc gccgcccggc tgcttggccg
     9481 cgtcctgcat cgactcggcc gccttgtagc gcatgtactt gtccatgtcg cccagagcgc
     9541 ccatcgacgc ccgctcgtcg atcttcttct gcacttcctc gggcggcgta atcgccgaga
     9601 tgagcatgtc aaccagctca agcccctgct tgctgaagtc ctcggccacg cgcgccttca
     9661 ccccggccgc cagctcatcg tagtaggccg gaaggtctaa gacggtcttc aagttctcgc
     9721 cgagcaaatc gttcaggcgc gagacgacga tgtcgcgcag gaagtcggcg atggagttcg
     9781 tcgagacgat gccgcgcgtg ccgacgatgg agccgacgaa gagctgcggg tcggcgacgc
     9841 ggaaggagta cttgccaaac gcgcgcaggc gcaccatgaa cagctcggag tcgcgaaacg
     9901 ggatcggctc cttcgtcccc cacttcatgt cctgaaaggt gtggcgcgcg acgaagacga
     9961 cggccgcctg aaacggcgac gtgccgccga acgggatcga cagcagccgc gtcagcagcg
    10021 gcacgttcat ggtggtgagc gtgtggcggc cggggccgaa cgtgtcgagc gccttgccgt
    10081 cgcggtagaa gacggccgac tggttctcct ggacgatgag ctgcgcgccc atcttgatgt
    10141 ccgtggcgcc cgactccggg atgcggtgga ctactgccgc gcccgtcggg tcgaagtact
    10201 gcaagatttc gagtgccatt ccgtttgctt ctccctcagt taaagtgagc agtgagccgt
    10261 aagcagtgag cagtggaaga cagtttttaa ctgcttactg cttacggctc accgctcact
    10321 tcttgaatct gatcgagccg ttgacgttct tgaccgtgag cggcgccccg ccatcgccga
    10381 gacgcgtgtc cacgtgccag cccggttgcc gcttctgcgc ctgaaagcct agctggttgt
    10441 caacgtcgat actaccgttg acgatggtcg ccttcaaatc agcgtccatg ccttcgatga
    10501 ggcggagggt cacgtcgccg ttgacgttga tgaattcgtg cggcccctcg cgctccgcgt
    10561 cgcgatagac gacctcgatg tcgccgttaa cgagcttcgc ctcgatgggg ccgcgcacgt
    10621 cgtcgagtgc gatgtcgccg ttctggaggg tgacgccgag cgagcctgta aacttcgaca
    10681 cctgcacgtc gcccgagcgc gaggagagtt ccatgcgttc gagcgagcgc ggcagcttca
    10741 cctcgtagga aattttcacc tgccggttgt cggcggagga gagcgccagc agctcgtcgt
    10801 cctgcgtgag cctcacgcgc gccgcctgcc ggtcttcagc cgagccgccg cgcttcgtca
    10861 ccttcagctc gacgcgatcc tcgtcccagc cctcgaccac gatgtcgccc tgcgtgttct
    10921 tgagcgtgaa gaccgcgtcg tcgcccagtt caaaggtctt ggtcagcacg gtctcgtcgg
    10981 cggtcacgat ggccccggcc tcatcgagca cgctttcacc gggcgcaaga cccggcagtc
    11041 ccggcggcgc gggcggcgcg ggcgggggca tacccctttc cacggcggcc atcctcctct
    11101 gcgccccccg gaacaccacc acgccgagcg agacgcccag cactgcgatg agcagcaggc
    11161 cgagcagcgc agggatgagc cagcgtcgtc ccttcgacgg ctgcgccgcc gccggcgcgg
    11221 acgcgagcgg cgaggtgtgt gcgaagctcg ggaagggcgt ctgcggcccc gtgtgtcgcg
    11281 gcccccacag acccgcgccc gtgccgcgct gcggcggtag ggtcgtcgtg gccgaggccg
    11341 acggcggagg cggcgcagcc gtgtcccgga gaatctgcgt cggcgcttct cccgccagcg
    11401 gcgccgtggg cgcaccgcag cgccggcaga actgctgccc cgtgaacaac tccgtgccgc
    11461 aatttgtaca gcgttcagtc atggcaaaga agaggtcggt agttcagatt tcagaggctt
    11521 caccgaagac cttgacggtt ttccgacctc tgacctctaa acacctccgg ccccactttt
    11581 attgcacccc gaacatgggg ccgggccgcc cgccctgcgg gccgtcttcc ggcgagccgc
    11641 ccccgccgcc ggtgtcgttg atcgagcgcg agcgctcgtc gaaggcgcgg ttgaactcgt
    11701 cgagcgcgcg ctccagattg gcggcggccc ccttgagtcc ttcggcggtg cccgcgccgt
    11761 ccttgagcgc gcgcgcgtgt tgctccagct cctcgacctt ctggacgagc gcgaggtcga
    11821 actgatagag ccggtcgagc tgcggctcct cgatcttgac cacgtcgaag aggccgccat
    11881 agccgtaact ggcgtagcgg acgcgattct cgaccatgtc aactttcttc gcggcgcggt
    11941 caacggggcc gacttccatc aggcgacggc cgtcggtcag ctccctcacc acgtcgttca
    12001 aggtctgctt cagtgtccgc aggcggtcgg ccagatgctc gcggtgcagc ttgtcgatgt
    12061 cgcgccgccg ctccttctgg acgtagccgc cgtagccggg aatcttctcg ccgagcctct
    12121 caagccagtt gcgctggtcg ttcatttgcg tctcctgaaa gagctgttag ctattagctg
    12181 ttagctatca gccattagta aagcgaacaa agggatcgtc ttcagcgtcg ccttcacacg
    12241 cgacggcgtg cccggccgtc tcccgccttg agctgaaagc taacagctaa tagctaacag
    12301 ctctatcaat acgtcctgaa gccggggcgg gttgcgattc taacaccgcc ggggccgaag
    12361 gcgtaaagtt gttgcgtgcg gcgcgcgtcg ggctttatta tagacgcgtc cccgcacatt
    12421 tgcagactct gcagccgcgg cccggccggc gacgcgtctc ccggccgggc tacgggggta
    12481 aaaaaacggg cgggcgcggc cggcacgaag catcccactc gaattcaagt ccatcgggaa
    12541 acgaacgaac atgagcgaag agaccgacca gcacacctcg caggccccca cgcccgaggg
    12601 caaatacgtc tactgcatca tcgagaccaa ggagccgcgc aacttcggcc ccatcggcat
    12661 cggcgggcgc ggcgacgaca tctacaccgt ccaccacgac ggcctcgcgg ccgtggtctc
    12721 caacacactg cttcaggtct acgacccgac gcgcgagaac gtcttcgccc acgagcaggt
    12781 caacgagacc gtgatgcgcg agttcaccgt gctgccgatg gccttcggcg tcctcttccg
    12841 caccgaggaa gacatcatcg agctgatgcg cggcacctac gacgccctgc gcgacgtgct
    12901 cgccaagatg gaaggcaagg tcgagttcgg cctcaaggtc aactgggatc gcgagcgcgt
    12961 catggccgaa ctcgaacagg agagcgacga gatacgcaac ctcaaggagc agatcacctc
    13021 gcgcgccacc ggctccactt acttcgcccg catgcagctc ggccgcctca tcgagaacgc
    13081 gctcaacgac aagtccgagg gctacgtcca cgaagtctat gccgtgctgc gcgacacggc
    13141 cgtcgcctcg cgcgccaaca agcccatcgg cgaccgcatg atcatgaacg ccgccttcct
    13201 cgtcgagcgc gagcgcgagc ctgagttcga cgcccacgtc aaacaggtcg ccgccaagta
    13261 cgaggacaag ctctccttca aatacaccgg cccctggccg ccctacaact tcgtccacat
    13321 ccgcctcaag ctcgaacgcg gcgagggcgc ggcggatgcg atacagaagt agggagtcag
    13381 gagtcagaag tcaggaatca gaatggagcg ctcacccgcc cggactttta ctgacctgat
    13441 cgtgtggcag aaggcgcatc agtttgtgtt gtcggtctat cgattcagcg attgctttcc
    13501 gcaaaaggct cgctcgaaga atgtcgctac tacttgattc tatcgaccga cctgaaatac
    13561 ggcgacggcc gagaactcct gtcgcgactc gaagaagtca gcaggctact cgaaaggtat
    13621 caagcggcta ttctttcttc tgactcctga ctcctgactt ctgactccct atgttcttca
    13681 tcgacgacat cctgctcgcg cccgtcaacg gcttcaagtt catcctgcgt cagatcgagg
    13741 agctggccaa tcgtgagctg aacgacgaca cggcgatcaa ggagcagttg cttgagttgc
    13801 agatgcgtct ggagctgggc gaggtgtcgg acgaggagta cgtcgaggcc gagcgggagt
    13861 tgtttgcgcg tttgcgggtc atcaaggagc ggcagttggc ggcgctgtcg caggtacaca
    13921 cggcggagag ttcgacgatg acggtcgagg cgggcggcga cgagggcggc gagttctggg
    13981 agccgggcgg aggctcggag cgttgacgcg ccgggagcgc gccggcggcg gaggggcgcg
    14041 cgccgcgccc cggcccgcgc gcaagtcgtc tcaaaagaaa tcctccaaat cgccgcgacg
    14101 aaaagcctcc gcgccggacg cgtctctcgc acccgcgacc gattttcccg ccgcgcgttt
    14161 tctcttcttc ggcggcaagg gcggtgtcgg caagaccacg gccgcctgcg ccgccgcgct
    14221 tctcctgctc gaacgagccg ccgccggcga gcgtcttctc ctcttctcca ccgacccggc
    14281 acactccctc tccgacagtc tcgacacaag cgtcggcgac cgcctcatcg aagtcacgcc
    14341 gcgcgcgctc cggcgcaccg ctgctaacgc cccgcgactt ttcgcacgcg agatggacgc
    14401 ggcggccgcg ctcgaagagt tcaagacgcg gcaccgcgcc gtgctcaaag agatcgccga
    14461 ccgcggcacg ctcttagacg aggccgacat caacgacctg ctcgatctct ccctgcccgg
    14521 catggacgag gtgatgtcgc tcttcgagtt gagcgagctg gcgcgcggcg gcgagtacgc
    14581 gcgcgtcgtc gtggacacgg cgccgtcggg ccacacttct cgcctgctgc gcctgcccga
    14641 gacgttcgcg cgctggacgc tcgcgctcga ccgcatgaac gagaagcacc gctacctgct
    14701 cgcccacttc gtccgcggcc gacgcttcgc ggcagacgac gtggagctgt tcctgcgcga
    14761 actcacggag cgcgtcgccg ccgtccgcgc gacgctcttc gatgcggccg gcgcgagctt
    14821 cacgctcgtc accatccccg aagcgatgag cgtcgaggag agcgcacgct actaccgcct
    14881 gctgaaagat gagggcgtgc ccgtcaccga cctcatcgtc aaccgcgtcg agcgcgagca
    14941 cgacgcttgt ccatattgcc gcgcacgcgc acgcgcgcag tcgccgtggc tcgccgagat
    15001 tgaacgtgag ttcaaagacc ttcacatccg ccgcgtgccg ctcctccccg aagaagtgcg
    15061 cggggacgtg cgcctgcgac gcttcgccgc gctcgcgtgg ggcacgggcg gaagcccgac
    15121 cgtgagggag ggcgtaaagg ctacgccgcc gagcgctcgc cctgccaaaa aacgccctcc
    15181 ctcacggtcg ggcttccgcc tcggcgagaa gcgcctcgtc atcttcggcg gcaagggcgg
    15241 cgtgggcaag acgacggccg ccgcctcggc cgcgctcgcg ctcgccgaaa gtgaccccgc
    15301 gcgccgcgtg ctcgtcttct cgaccgaccc ggcgcactcg ctctccgaca gcttcgacga
    15361 agaggtcggc gcgctcaaag agggcgtcgc cggtgtcggg aatctggacg cgatggagat
    15421 cgacccggcg gtgcgcttcg aggagttgaa ggatcgttat cgacgctgga cggatgagct
    15481 gttcgactcg ctcacgggcg gcacgcgctg ggaggtgaag ttcgaccgcg aggccatgcg
    15541 cgagatggtc tcgctcgccc cgcccggcat cgatgagatc gccgcgctga gcgccgtcag
    15601 cgacctcatc gacgcggaca cataccagac catcgtgctc gacacggcgc ccacggggca
    15661 cctcctgcgc ttcctcgaac tgcccgaggt cgcgctctcg tgggtcagga ctttcatcaa
    15721 gctcatgctg aagtacaagg gactggtgcg ctacggcagc gtggcggagg agctggtcgc
    15781 gctctcgaag tcgatcaagc gcgtctcggc gctgctcggc gacgcgggcg agtgcgagtt
    15841 cgtgggcgtc gccattcccg agacgatgag tctggaagag acggcgcgtc tcgccgaaag
    15901 cctcgcgcgg ctcggagttc cgatgcgccg gcttctcgtt aacaacgtcg tgtcggcgga
    15961 ggcggcccta gcttgcgact tctgcgccgc gcgccacgcg gcgcaggcgc cggtgctaaa
    16021 aaacttcgcg gagaggttcg acggcgcggt agaattgttc ctcgcgccgc agcagccgca
    16081 cgacatccac ggccccgcac ttttgcacga acacttcgag cagtggcgaa gcgcaagttg
    16141 aaagtacgcc cgaggcgcgc gccgaagagt aaaccgaatg agcgacagcg cgacaaagaa
    16201 aaacgcgggc ggcgacggcc ccgaagaggc cgcggccctc tacgtctact gcgtcggcga
    16261 gcgcgcggag cttgagccgc tgctctccga cgagggcgtg ccggcggcca tcgaagagtc
    16321 gggcgcactg gagacggtcg gcgcgggcgc gctggcggcg gtcgtgagcg ccgtgccgct
    16381 cgcggactac ggcgaggagg cgctcgaagc gcgcatgagg gatgcgacgt ggacggccac
    16441 gcgggcgatg cggcacgagc gcgccgtcga gttcttcgcg cgccgcgcga gcgtcgtccc
    16501 cctgcgcttc ggcaccatct acctgacgcg cgctcgcgtc gccgccatgc ttgatgagag
    16561 agacgctgag ctaagatcgg ccgccggccg cctgcggggc cgcgaggagt ggggcgtcaa
    16621 cgtctatttc gaccgcgcgc gtttgaagga gatggtcgtc gagttgagtc cgcgtctgcg
    16681 cgcgctggcc ggtcaggcgt cggccgccgc gccgggtcag tcgtacctgc tgcgtaaaaa
    16741 gatcgaggcg atgcgtgccg acgaggcgcg tgccgaagtg cggcgcgtcg ccggcgaagt
    16801 tgaaacaaaa ctcgccgcga cggccgcgag cgccgcgcgt ctgcgcgtcc tcaaggacga
    16861 ggcgagcgag cacggcgacg tggcggcgaa gctcgccttc ctcgtcgagc gcgaacgctt
    16921 ccccgagttt cgcgacgcgg ccgaagccct cgcgcaaaag tacgagccgg ccgggttccg
    16981 tctggagctg accggcccgt ggcccgctta caacttcgcg gcgggcgaat gaaagaaaag
    17041 ggtaaagggt aaaggggaag gggtcagggg taaaggggaa aggtagtgga gcggctttag
    17101 tcccttcccc tttacccctg accccttccc cacagtttgt tatgaccgaa gagctggagt
    17161 tcacagggtt cgacgaggac gaggagctgt cgctgctgga gacgctcaac cacgtgctcg
    17221 acaagggcgt ggtggtcgcg ggcgacatca ccatctcggt ggcgaacatc gacctcatct
    17281 tcgtcaacct caacgccctg ctcggctcgg tcgagaccgt caacgaagtt ctgagccgcc
    17341 ggcgcgaccc cgcgcccgcg ccggcccttg ctgcgcgcgc tcacgatgaa ggcgcgaagg
    17401 ctgatgaaaa gaaggtggac acgcggcgcg agagcggggt actcttgacc gagcagttcc
    17461 ccctgccccc agagccttta gagtcggaag tttcggacgc gcgcgcgggc gccgcggtcg
    17521 aacagctccg ccgcgaaacc ggacgcctgc ccgagcgcat caacatcgac ccgcgctcgg
    17581 tcgagcaggg tctggcgaag ctggtgctga cgctcatcga attgatccgc agattgttgg
    17641 agaagcaagc cgtcagacgg atggaaggcg gcaggctcaa cgacgcgcag gtcgaagagc
    17701 tggggctggc gctgatgaag ctcgaagaga agatgcgcga gctgaagagt cagttcgggc
    17761 tggaagacga agacttgaac ctcgacctcg gccccatcgg acgtttaatc tgaggggccg
    17821 tgttcgggag cggtaaaaac tacaggcagt cgcggtcgcg cgctcttcgg ttttccgtcg
    17881 aggctgcccc ccgagtcgaa gggagagacg agagttgtca agcgagcgga cgagtttgat
    17941 ctcggcgcgc ccaccgggcc tcgtggacgt gctcgaccga attctcgaca aggggctggt
    18001 gatcgcgggc gatgttaaag tctcgctcgc caacgtcgag ctgttgacgc tccagatacg
    18061 cctgctcatc tgctccatcg acaaggccga gcagatcggg ctgaactggt ggcgctacga
    18121 ctcgaacctg acgacgcgcg ccgagcgcgc cgagactgaa aacctcgaac tgcgcgagcg
    18181 gctcgcggcg ctcgaacgcg agatcaggcg actggacgcc catgcccccg ccgccccggt
    18241 cgcccaaacg gccgccggcc ccctgaagcg cgccgcaagc aagccggccg gccccccgtc
    18301 gcgcaagggc cgcaaccgtt ccccgtaaat ctttctcctc tgaaaagccg cggccgtctg
    18361 aacgaaggcg cccaggacga gcgcggcgcc gggagcgcgc gccggaggta atgggcctcg
    18421 ccacccgtta aattttcaat agaccgaaga gaggaaaccg aaaagaaatg gctgttgaga
    18481 gagcgccggg cggaaccagt ctgatcgatg tcctcgaccg cgtgctggac aagggcatcg
    18541 tcatcgacgc gtgggtgcgc atctcgctcg tcggcatcga cctgatcacg gtggaggcgc
    18601 gcgtcgtcgt cgcctccatc gacacctacc tcaagtacgc cgaggccgtc ggcctgacgc
    18661 cggccgtctc gcgccccgcc atggggccgg ccccggcggc ggccataggg cctgccccag
    18721 tggcggccga gccaatcggc gaagagacgc gccgacgcac caccatcgag gtggaacgac
    18781 agtaagacga gaattcagga gtcaggagtc agaagacaga atgaagaagg gtcacgcctt
    18841 tcattctgac tcctgactcc tgactcctga ctcctgcttt tatggatcgt ctcacggaaa
    18901 cgtcacaggc ggagccggcc gcggcggggg tagcgggcgg cgacgcgcgc gcgcgcacac
    18961 cggagacgga acgccactac gagctgctgt ttgagacgac gacgcaactc ctcgccgcgc
    19021 cgaccgtcga ggagcgaatg ctcctgacgc tcgacgccgt cacctccggc ctcggctacc
    19081 cgcacgcggc cgtcgccctc atcgacaagc gtcaggtcac actcaaagtc cgcatggccg
    19141 tcggcttcgt ggacgacgcg gccgtctgcg atgtagagac gccgctcgac gctaacgggc
    19201 cgcacgtctc ggtcgtccac gacggacacc ccgcatgggt cacccgccgc gccggcgagc
    19261 cggcctccgt cgccttcctc gaccgcttgg gatgtcgcga cacagacctg ctcgcgctcc
    19321 cactcttcgg cggacagccc ggcgtggccg agaagaccgg aggcgagttc gcccgccgcc
    19381 cctacgacga cgagcgctac tggatgcccg aggcgatgtg catgggcgcc ctctatgtcg
    19441 gcgcgcttca agtcgagatg ggcgaggagc agtatcgcct gctcacacgc ctcgccgacc
    19501 gcgtcggcgt catcctctcg gccgccgtcc agatggagca gttgaactcg tcggtgcgca
    19561 agctccagcg cgagcggcag tgggtggagt ccgtcatgca gtcggtggcc gaccccatcg
    19621 tcctgaccaa cctcgacaac gaaatccttc tccagaatcg ccgcgccgaa caactcttca
    19681 gcggtcagaa gaacgcctcg cccggcaagc gccgcgcgct ggagatgaac gacctgctgt
    19741 tttcggccta cctgtcgagc gcggccgtcg cctccgaggg gatcgtcgcg cgcgacctca
    19801 ccttagttga ccccatcgac ggctccgaca tccacttcga ggtcgtctcc acacccgcgc
    19861 tcgacacacg cgccgagcgc atcggcctcg tctccgtctt ccgcgacgtg accgacctgc
    19921 gccacgccaa cgaggagctg gcgcgcaact tcgccaagct ccagcaggcc gaacaggagg
    19981 cccggcgcga gcgcgaccgc ctcgacctga ttctggaaaa cgtcggccac cccgtcgccg
    20041 tctccgaccc ggccggcaac ttcatcctct tcaactcgcg cgccgagcgg ctctttcagg
    20101 agcaggccga catggcgccg cgcgccgcac gcgccgcacg cgccaacgcc gtcaagctca
    20161 cctccttcat ctccggtctc gcctccgagg ccggcagcgg ccgccgcgac gagatcgccc
    20221 tcatcgaccc cgagctgggc gaatcactcc cgatggaaat caccgccgcc gaagtcaccg
    20281 acgcgcgcgg gcaggtgacg gccgtcgtct ccatccttca cgacctgacg gagattcgcg
    20341 agcttgaacg ccgccgcgtc gagcagcagc tcttcgagtc ggagaagctg gcggccgtcg
    20401 ggcgtctggc cgcctccatc gcgcacgagg tcaacaaccc gctcgaagcc atcaagaact
    20461 cgctctacct cctgacgacg agccagaatc aggactccaa cacgcgcttc ctcgaagtcg
    20521 cccgcaagga gaccgagcgc gtctcgcaca tcatccggca gatgctggga tttgcgcgcc
    20581 gctcgggcga ggtcgagccg acggacgtta accagatcat cgaggagacg ctggttctgg
    20641 tcgagaagaa gatgaagcag gcgcgtgtgc gcgtcgcgcg cgaactcgaa gcggggctgc
    20701 cgcaggtgcg cgcccgcgcc gaccagctcc ggcaggtctt cctcaacctc atcctcaacg
    20761 cgcagcagtc aatcgacggc gagggcgagg tgcgtatcca gacgggccgc tacgatcagg
    20821 cgctccagcc ctcgatctcg gttcgcatca gcgacaccgg caagggcatc agcgagcagg
    20881 acgtggcgcg catcttcgag ccgttcttct ccacccgcgc caagggcacg ggcctcggac
    20941 tctgggtctc gcaggacatc gtgcgccacc acggcgggcg catcgaggtg acgagcatcg
    21001 aaggccaggg caccaccttc aacatcatcc tccccatcga ctcgccgacg ctcacggaga
    21061 cggatgtcag cccaaagaag cactaacggc gacgggacga caggagcgct cggcggcagc
    21121 ggccggcgcg cgcccacgag gaaacgcgtc ctcgtcgtgg acgacgagga gaacgtgcgc
    21181 gtcaccaccg ccgccatcct cgaacaggat ggctactacg ttgacacggc cggcgacggc
    21241 cgcgaggccc ttcagaaggt ggagcgcggc ggcttcgacc tcgtgctcac cgacctccgc
    21301 atggacgaca tggacggcgc cgcgctcttg aacgagctgc acacacgcca ccccagcgtc
    21361 gtcaccgtgg tcttgacggg ctacgcctcc atcgagtcgt ccatcgacgc gctgcgtcag
    21421 ggcgtctacg actatctcgt caagccgtgc gtcatcgaag acctgaagcg caccgtccgg
    21481 cgcgcgctcg aacaccgcga ccagaaggcg cgcatctcgg agctgtcgaa cccggtggtc
    21541 gaaatctggg acggcatcct gttgcttccg ctcgtcggca cgctcgacga ccagcgctcc
    21601 tcgcagatgt ccaccacgct cctcaacgcc gtccgcgcca agtccgcgca ggccgtcatc
    21661 atcgacatca ccggctgcac ggtggtggac acctacacgg tcgcgcacct catcaacacg
    21721 gtgcgctcgg tgcatctgct cggcgcgacg ggcgtcatca cgggcgtcgg ggcctccatg
    21781 gcctccgacc tcgtcaagct cggcatcgac ctccacgaaa tcaacacgcg ccgccgcctt
    21841 gccgacggcc tgcgcttcgc ctttgaactg cttaacgtgg gaatcagagg tcagaggtca
    21901 gaggtcggag gtcagaaaaa acgcgacctc ttcactgacc tctaacctcc aacctctgac
    21961 ctctgacttt ctatgtcttc cctcgaccct acacaaacga acggccatga ggccacgcgg
    22021 ctgctcgtcg tggacgacga ggagagcgtg gcgatcacgg tgagcgaggt gctccgtctc
    22081 gaaggcttcg aggtggacac ggcgatgagc ggcgccgacg ccgccgtgca actcggccgc
    22141 gcgcactacg acctcgtcct taccgacctc cacatggagg agggcgacgg catctccgtg
    22201 ctcgaagagg tgcgccgtgt gtcgcccatg acgatttcga ttgtgctcac gggcttcgcc
    22261 tccgtcgagt cggccatcgc cgcgctacgc aaaggggcct acgactatct cgtcaagccc
    22321 tgcatcatcg aggagctgaa gctcaccgtc cgcagagggc tggaacaccg ccgcctgatg
    22381 ctcgccgagc agagcgcccg gctcgggctt caggacctta accgcgaact cgaacagcgc
    22441 gtgctggatc gcaccgcgga actccagcgg ctcaacgacg aactcctcga agccaaccgc
    22501 gccaaggacg ttttcctcgc cacgctctcg cacgagctgc gcacgccttt gacgcccgtc
    22561 ctcggctggg tcaacctgct gcgcgcgacc ggcgcctcgg gcgacccgaa cctcctcgcc
    22621 caagggctcg acgccatcga ccgcaacgca cgccttcagg cgcgcctcat cgacgacctg
    22681 ctcgatatct cgcgcatcat ctcgggcaag ctccggctgg agtgggaggt ggtcgatctg
    22741 tgtcaggtcg tcgaggcggc ggccgagacc gtgcgcgggt cggccgagga gaagggcgtc
    22801 cggctcgaag tgcagacgag cgccgcgccg ctcgtcgtca gaggcgcgag cgtccggctc
    22861 cagcagatcg tctggaattt gctggcgaac gccgtcaagt tcacgccgcc ggcgggcgtc
    22921 gtctccttaa ggctggcgcg cgagggacgg gaggcgcgcg tcgtcgtcga agacacgggg
    22981 tcaggcatcg cgcccgagtt tctgccgcac gtcttcgacc gcttccggca ggccgacggc
    23041 tcgaccacgc gccagcacgg cggccttggc ctcggcctcg caatcgtgcg cgcgctcgcc
    23101 gatttgcacg gcgggcgcgt tgaggccgag tcagaggggc cggggcacgg ctcgcgtttc
    23161 atcttcgcac tgccctgcgc cgcctcggcc gccgtcatcg acgaggcgat ggccagcgtg
    23221 gatgcgccca cgctcgcgcg gcccgtcctc gtcgtggacg actcgccgga gacgctcgaa
    23281 ctcctcgaaa tgttcttctc gcgcaagggc tacgaggtgt tgaccgccga ctccgccgag
    23341 cgcgccctcg aactcgcgcg cgccgagccg cccgccctcg tcatctccga catcagcatg
    23401 cccaacgtgg acggctacca gctcctcgcg cggctgcgag agctgcccgg cctcggcgac
    23461 gtgcccgcca tcgccctctc cggctacgcc atggaggagg atcgccagca cgccctcgaa
    23521 gccggcttcg ccgtccacat cgccaagccc gtagaccccg acgaactcct cgcgaccatc
    23581 cagcagctca cctcctctga agaacaagga tgaagaagaa gtaaatcgtg aatcgtaaat
    23641 cgtgaatcgt gaaagacagg ttcttcgatt cacgatttac catttacgat tcacggcctt
    23701 tcattcatcc ctcatccttg ctatttcccg gccccggtaa tatgctgcgc ccgctcatac
    23761 gccggcaaag atgtcttcgc cgatttgaac ccgagtgatg cgcgtctggc tttcccgtgt
    23821 ctcacgtttt cggtggtggg tcgagtacaa atcgagcctc tacacctcat cgtccaccgc
    23881 acaggcgtcg tcgcgaaatg attctactca gagacgcata acgcacagat gcgccgggag
    23941 aaatgttcga ctctgctccg tcgatcagac ctggaaatga ctaaccactc acgtttcagg
    24001 cttgcaaacc cagacgccga cggccccgtc tgctcaggcg accacaggcc cctcccaatg
    24061 agcacaatct cttaaagtcg agcgcgccgg cacggacggt cgcggcggcg ttccggtctg
    24121 cccagccccg gccttcgtcc cgcacgcggc gcgttcggcc cgtccgccgg cccggccggc
    24181 agctttcaac gcgtgcgacg ccagcgtccg acaagttgag aagggaacat aaggagcgtt
    24241 tatgaccacg aaaagtaatc ggaaagccgg cggcggcaag cgagccggca agagcgcggc
    24301 aggtgcgaag aaggcgagcg gcaaaacggc cgcgcgcaag aaggcaggca agaggaagca
    24361 ggcggtggag cgcgaagagg ggccgctcgg ctggctcgtc cccacgctgg aagcttccta
    24421 cacgcggctg gtcccgcgcg aagagtatga ggccgcacag gcggaggccg ccagggatgc
    24481 cgccgctaag aaggctgccg gcaagaaggc cggcgccaag aaagccggtg ctaagaaagc
    24541 tggggccagg aaagccggct ctaagaaatc cggggccgcg ggtcccgaag cgttcgagag
    24601 cacgctccag ccgggcgagg gcgagtcggt tctggcggac gtggcggata atctctgggt
    24661 ggatcggctg gccgagtaca agcgccgcaa ggcggcgagc gcgctcggcc tggggggagc
    24721 accggcggca gtggcaagcg gcgccgctag ggcgggcgcg gcgccgctgc cgcccggccc
    24781 tgtcgtaccg gggcagaaga actgggggcc gctggggccg tccgtcgtca tggaagcgca
    24841 ggcgcatgga ctgcccgccg tcggcggccg cgtctccgga atagccatcg cgccgggcgg
    24901 gcagctcgtc tacgccgcca ccgccaacgg cggcgtcttc cgctccgaag acgccggcat
    24961 ctcctggcgc tcgctcatgg acgccttcga tgtcgatccc accaccttcg ccagcaccag
    25021 tctggcctgc ggcgccatcg ccatcgaccg caacgacccg aaacgcatct acgtcggcac
    25081 gggcgagggc gacacccacg ggatgttcca gcagcgcatc atcaacgccc tgcccgccta
    25141 ccgcggcatc ggccccatcc gcagcgacga cggcggccag aactgggtcg aggagccgac
    25201 cgcttcgggg tcgcccacgc tcgccggcaa agccttcttc gcgctcgccg tcgatcccgc
    25261 caaccgcgaa tcggtcgtcg gcgcaacgac cgacgggctt taccagcgca cgctggccac
    25321 gggcggcgcg cctagctgga cgagacgacg cgccggcctg ttttcgagcg tcgtcgtcgc
    25381 cacttcggga agcacgaagc gtttcttcgc ggccgagtgg ggccggggcg tcatccgctc
    25441 ctcgaacggc tcctcgtggt caacgctcgg cgccgggttc cccaccacga acgtcgggcg
    25501 catcgccctc gcggtgcagc ccaacaaccc gaacctcgtc tacgccttcg tcgccgacgc
    25561 gaacggcgcg agccgcggcg tgttccgtct gaacagcgtc acgggggcgt ggaagcaggt
    25621 caccggcgtg ccctccgtgc tgccctcgcc gcagggggat tacgacctcg ccatcgccgt
    25681 cgatccgacg gacgccgacc tgatttatct cggcggcagc tacttccacg acaacgagtt
    25741 ctggcccgcc tccgtctggc gctgccgcgt caaggccgtc ggctcgggct tcacgatgag
    25801 cggcgtctcc atcggcaaac acgcgcacgc cgacgtacac gtcctcacac attcgccgaa
    25861 tgaccccaac gccctttggg tcgggtgcga cggcggcgtc ttcctcaacc gcgacccgcg
    25921 cggcacgggc atcttcgcca gccgcaacaa cggcctgtcg tgtctctgca cgaacttctt
    25981 cgcgcaacat cccaccgacc ccaacatcat cttctgcggc cttcaggaca acggcacggc
    26041 gcgcaccggc ggcggcccca tctggagaca cgtcaactgg ggcgacggcg gctactgcct
    26101 gatcaactgg gccgacccgc ggcaggtgct cgtcttcgcc aacggcagca tctaccgggc
    26161 cacggacggc ggcctcagtc acaactcctg gtcgcgcacc gagttcgatt gggcgacgat
    26221 gaccgagcct atcgtcgggc cgccgttcaa cccctcgaag cccgcggagg caaagctcgt
    26281 ggcgctcggc gcgggccgtc aggtgcgtct gtcgaaagac ttcggcgcct cgtggttggc
    26341 ggccgacatc gtctcgctgc ccggcgccga cccctcgggg agcatcttct ccctctcctt
    26401 cgcctcggcc tcgcgcttct tcgtcggcac gacggccggc gatgtcttcc gcgtggacaa
    26461 gtccggcagc tcgtggacgg cgacgcgcat cgacaacgcc ggcgcggggc cgctcccgct
    26521 gcgcggcctc atctccgaca tcgccgtcga ttgggccgac cccacgctca gctccatcta
    26581 cgtcgccttc ggcggcatgg gcgatttccg gcacgtctgg cgcttcaacg gcacgcgctg
    26641 gcaggcgcgc agcggcgcgg ccaccggctc caccaacctg ctcgacgttg agcacaacgc
    26701 catcaccgtg gacaagagcg ccccggcgaa cgtctacgtc ggcgccgaca tcggcgtctg
    26761 gcactccagg gacggcggac tgaactgggt gactctgccc aacggcctgc ccgacgcgcc
    26821 gatcttcgac ctccagaccc acccgacgcg gcgaatcctg cgggccgcca cgcacgggcg
    26881 gggcatttac gaatatccgc tcgaccccgc gtcgtaagat gtgagggccg cgcgtcgcgc
    26941 cgttgcgacg cgcggccctc gcgaacaagc tggggaggta atccgtcatg ctccagacat
    27001 tggcgacagc ctatgtcctc gttctcgtcg ccgagctggt cggcgacaag ctcatctata
    27061 cggccggctc actgacgacg cgcttccgcg cgctgccggt cttctgcggt ctgtcgctcg
    27121 cgttcatgtt gaagatgctg gcggcggtga gcgtcggccg tttcatcgcc gagttgccgg
    27181 gcgcggtggt ggccggcgtg agcgccgcca cgttcttcgc cgcggcggcc gtcatctggt
    27241 tccggcgcgg cgggggcgag gcgtcgccga aggcggaggc cgaggcaggg tggtggcgcg
    27301 ccgtcggcgt ctccttcgcc gccgcgttcc tcaccgagtg gggcgacatc ggacagctca
    27361 cggccgccac gctcgccgcg cgccacaacg cgccgctcgt cgtctggttc ggcgcgaccc
    27421 tcgcgctgat gaccaaaggc gcgctcgccg tcacgctcgg cgtgggcctg cgccgtaagg
    27481 ttcccgcccg cgttctgcgc cccctcgccg cctccgtctg cgtcgtgatg ggcttggtct
    27541 ccgcgcacgg agtattcaga taaggcaaaa aaggccgtaa atcgtaaatc gtaaatcgtg
    27601 aatcgaagaa cctgtctttt actatttacg atttaccatt tacgatttac ggcctttttt
    27661 gccttgtccg cgcgctgaga accgtgtggg cgacgaccgc cgcgatgatg acggcgccgc
    27721 ccgcgatggc ccagggcgag gggcgctcgc cgatgaagag gtagacccac acggggttcg
    27781 acaccggctc gacgtagccg accagacccg cgtcgagcga gcgcacgccg cgctcgatgc
    27841 cgcgcgtgaa caaggtgtag gccacgccga tctgg
//
LOCUS       DASVES010000217         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_921363, whole genome shotgun sequence.
ACCESSION   DASVES010000217 DASVES010000000
VERSION     DASVES010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921363"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1905)
                     /locus_tag="VF240_08745"
     CDS             complement(61..1905)
                     /locus_tag="VF240_08745"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEX7175331.1"
                     /translation="MSLHPPFVGRSTTRLFNLRLRKFSWPALALVLLATLMTWTGSAA
                     AACNFLLKFGSTGSGPGQFLNPRGIAVDNAGNVYVPDENNRVQKFDSSGVFVLQFGVP
                     GDVNGGFVTPWKVATDAAGNVYASDSFTNRVQKFNSSGVFQMSLGGGPGSGNGQFIAG
                     TAGIAVDSAGNIYVADPGNHRVQKFSPAGAFITAWGGSGTGNGQFSAPGGMAVDASDN
                     IYVADTGNHRIQKFNSSGTFLTKWGSNGTATGQFNGPQGISVDTTGNVYVADTGNNRI
                     QKFSSTGVFIDSCGSAGTGDGELSGPHDVAADASGNIFVADTGNERIQKFGPAPATPI
                     ADAGPDQTVECAGALTSVTLDGTASTPGSGTINAYDWTEGATTLGSGSTLNVSLPAGS
                     HTITLTVTDTGGGTDSDDVIVNIVDTTDPIISCPANVVVMLPPNSSATSMAVDYPAVT
                     AMDSCSSSVTVESSPASGSVFPVGTTTVNATATDDAGNDSACSFTVTVNYNFAGFFSP
                     VNNLPVVNTVNAGRAIPVKFSLSGDKGLNIFAPNSPSSGPIACNSSDPAVVLEDTVTA
                     GGSSLSYDAASDQYVYIWKTSTAWAGTCRQLVVQLNDGSIYRANFKFK"
     gene            complement(2348..2992)
                     /locus_tag="VF240_08750"
     CDS             complement(2348..2992)
                     /locus_tag="VF240_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7175332.1"
                     /translation="MIAGLDAVGVESAAADDGRELPPVSVSVLIYERRLRGQSLMEFQ
                     LGHAKEILRRTPATLNSLLRHLPEEWVLANEGPESWSPFDVVGHLIHGEEADWIPRAR
                     IILECGESRPFETFDRFAMFEKSRGQSLGDLLDRFEWLRGENLKELEGMNLSPEMLGK
                     RGTHPQLGVVTLGHLLSTWVVHDLGHIGQVVRVMAKQYREAVGAWQAYLPVLSR"
     gene            complement(3284..3670)
                     /locus_tag="VF240_08755"
     CDS             complement(3284..3670)
                     /locus_tag="VF240_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175333.1"
                     /translation="MSALAGACEGGQPPEDLRPEITLENIREDINEKRVSLPAESDAS
                     EPSSWRFYPSEPKEIEILEKQLAGDRATFVIDLRTRTSPRAEQQGITKKLAGRLRLHY
                     ELQSYVVLRKWEIVRIENISFAYQKE"
     gene            complement(4032..4757)
                     /locus_tag="VF240_08760"
     CDS             complement(4032..4757)
                     /locus_tag="VF240_08760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7175334.1"
                     /translation="MRNFATRLVRKLAARAGNSQFIDISDEYVNWVCFANAGMLHRGN
                     LYCFDHAIRNLPSRAPIVEVGSFCGLSTNLLTYYKRKRGVSNQLITSDKWAFEGAQDG
                     RTLGDSPIGHADYRAFVKETYTRNIRMFSRDDLPYTVEMLSDELFGAWRRSEEVSDIL
                     GRSVRLGGPISFCYIDGNHTYEFAKRDFENCDKFLESAGFVLFDDSADGSGWDVCKVV
                     AEVQATGRYELVIKNPNYLFKKR"
     gene            complement(4862..>5061)
                     /locus_tag="VF240_08765"
     CDS             complement(4862..>5061)
                     /locus_tag="VF240_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175335.1"
                     /translation="TSRLLDKQVIRIAGEFSNGDTAFSFTDLGWVVQQRLKTGLRQYL
                     LQVESPPSDKDDGETSGEATA"
BASE COUNT         1056 a         1492 c         1433 g         1080 t
ORIGIN      
        1 caatttgaca ggggcgcccg gccacttacc tgacgcccct tgcgccagag cgccaactcc
       61 tcacttgaac ttaaagttcg cgcggtagat gctcccgtcg ttcaactgga ctaccagttg
      121 ccgacatgtt ccggcccatg ccgtgctcgt cttccagata tagacatact ggtctgacgc
      181 ggcatcgtag ctgaggctgc tgccgcccgc cgtcaccgtg tcttccaaaa caaccgcggg
      241 gtcggaggag ttgcacgcga ttggccctga agagggagag ttgggggcga agatattgag
      301 ccccttgtcc ccgctcaggc tgaacttcac gggaatcgcc ctgccggcat ttaccgtatt
      361 gaccaccggc agattgttga ccggcgagaa gaaccccgcg aagttatagt ttacggtcac
      421 ggtgaagctg caagcggaat cgttgcccgc gtcgtctgtc gccgtggcgt taaccgtcgt
      481 cgtaccgacg gggaagaccg agccggaagc cgggctgctc tcgaccgtca cactcgaaga
      541 gcaactgtcc atagccgtca ccgccggata atccacggcc atcgaggtcg ccgacgaatt
      601 cggcggcagc atgacgacga cgttggccgg acaggagatg atcgggtcgg tggtgtctac
      661 gatgttgacg atcacatcat cggagtctgt gcctccgccc gtgtcggtga cggtcagcgt
      721 gatggtgtgc gagcctgccg gcagtgagac gttcaacgtc gaacccgagc cgagcgtggt
      781 cgcgccctct gtccagtcgt aagcgttgat cgttccgctg cccggtgtgg aggccgtgcc
      841 gtcgagcgtg acggatgtca gcgcgcccgc gcactcgacg gtctgatccg gccctgcgtc
      901 ggcgatggga gtcgccgggg cggggccgaa cttctggatg cgttcgttgc ccgtgtctgc
      961 gacaaagatg ttaccggagg cgtccgccgc cacgtcatgc gggccactga gttcaccgtc
     1021 gccggtgccc gcgctgccgc aggagtctat gaagacgccg gttgaagaaa acttctggat
     1081 gcggttatta cccgtgtcgg caacgtagac gttgcccgtc gtgtcaaccg aaatcccctg
     1141 aggcccgttg aactgcccgg tggccgtgcc gttgctgccc cacttggtga ggaatgtgcc
     1201 tgacgagttg aatttctgga tgcggtgatt gcccgtatcg gccacgtaga tgttgtcgga
     1261 tgcgtcaacc gccattccgc cgggcgcgct gaattgcccg ttccccgtgc cagagccgcc
     1321 ccatgccgtg atgaacgcgc cggcaggcga gaacttctgg acacggtggt tgcccgggtc
     1381 ggcgacgtag atgttgcccg ccgaatcgac ggcgataccg gcagtgccgg cgatgaactg
     1441 gccgttgccg ctgccggggc cgccgcccaa cgacatttga aaaacgccgc tggagttgaa
     1501 tttctgtacc cggttggtga acgagtcgga cgcgtagaca ttgcctgccg catccgtggc
     1561 cactttccac ggggtgacga acccgccgtt cacatctccg gggacgccga actgcaacac
     1621 gaagacgcca ctggagtcaa acttctgtac gcgattattc tcgtccggga catacacatt
     1681 accggcgttg tcaacggcga tgccccgcgg gtttaaaaac tgtccggggc ctgagccggt
     1741 gcttccaaac ttcaggagga aattgcaggc cgccgccgcc gagcctgtcc atgtcatcaa
     1801 cgtggcgagg agcaccagag caagcgcggg ccagctaaat ttccttagac gcaaattgaa
     1861 caggcgtgtg gttgaacgac ctacgaacgg gggatgtaaa ctcatttcga actcctttgt
     1921 cgggggaagg tggataggtg tgcgcgcacc gggtaggtcg gatgcgcgtc gggtcgcatg
     1981 cggttcggga actcaaggac gcactctcgt cctccgttaa agacccatag cagttagaga
     2041 gttcgcgagg gaatcttccg gtgtttctat tttagctcga tacaacagac ccgccagaga
     2101 tagctcagat gactgcgagc ggctaccgag tcgattgtca cgcaggcaaa gaaacaagaa
     2161 cctcagaggg taggcacagt attcattatc gtgatagttg tcaagattta tcagagcagg
     2221 cgaaatggcc ggccgtcttg ccgcctgaga gtgtcggcaa ccccggatga ggcataaaga
     2281 attgtgagaa tctcttgtcg ctcgttgttc acttgaatga cttgttagct gcggcgctgc
     2341 tatccgacta tctactcagg acaggcaaat aggcctgcca tgctcctacc gcttcccgat
     2401 actgcttcgc catcactctg acgacctgcc cgatgtggcc gaggtcatgc actacccatg
     2461 tcgagagcag atggccgagt gtcaccaccc caagttgagg atgcgtcccc cgcttcccca
     2521 gcatctccgg gcttaagttc attccctcca actccttcaa attctctccc cggagccact
     2581 cgaacctgtc cagcagatcg cccaaactct ggcctctcga cttctcgaac atagcgaagc
     2641 ggtcgaatgt ctcgaaaggg cgactctcgc cacactccag gatgatcctg gcccgcggaa
     2701 tccaatcggc ttcctccccg tggatcagat gacccacaac atcaaagggg ctccagctct
     2761 cagggccttc gtttgcaaga acccactctt cagggaggtg gcgcaacagc gaattcagcg
     2821 tagcaggagt acgcctgaga atttctttag catgaccgag ttggaactcc atcagtgatt
     2881 gccctcgcaa acgtctttca taaatcagga cgcttaccga cacaggaggc agctcacgcc
     2941 cgtcatcagc cgccgcgctt tcaacgccga cagcgtcaag tccggcgatc ataagcgtct
     3001 tcggttcgat acagagcaag cttgacgcgc tggcgcacgg caacggtcta cgcttaacaa
     3061 gaggccactc gatactcaaa gcggcttgaa tgagtgcgcg ccgatgtggc cgactttcag
     3121 gttggggatg tagaaccgtg tacctccgcc gggcaaattc ttactgccgc ctactgaggg
     3181 actgcatacc gcccgacctt ctcgcgctcc cgatgctgtg cttcccccag gtcaataatc
     3241 ggaggctaca gattgtcgcc cggtgttgga gatgatgagc gggtcactcc ttctgatacg
     3301 cgaaagagat gttttctatt cgcacgatct cccacttccg cagcacaaca tacgattgca
     3361 actcgtaatg cagacgaagc ctgccggcca gctttttcgt aatcccttgc tgctccgcgc
     3421 gcggcgatgt gcgcgtccgc aggtcgatga cgaatgtcgc ccggtcgccc gcgagttgct
     3481 tctctaaaat ctctatctcc ttcggctcgg aggggtagaa tcgccagctt gagggttccg
     3541 aagcgtcgct ctccgccggc agactgaccc gcttttcgtt gatgtcttcg cgtatatttt
     3601 ccagagtgat ttcaggccgc aggtcttcgg gcggctggcc gccctcgcaa gcgcctgcta
     3661 acgctgacaa gagtaagagg agaagcaaag aaaattctcg gcggctcaca agtaagtcct
     3721 caacacggag ataagcattt gatcggcgcg cacagtacag gtggggctaa attaaatctg
     3781 cacatcgcaa cagttacaac cttgtgtgct ctcgccccga gccacagcta tgctgcaccg
     3841 cccacgaacg gggtcgccag aataatcgca tacaggcagc ccgtttttct agccccttaa
     3901 caagaggatg cctaacgctg gccttagagc cgcggcgcgc gatcagcatt caagctgaag
     3961 gaaaaggaat tatttgagaa acaggcaatc gcgccgtccg catgccgttg ttatgccgcg
     4021 gcgcgggtaa actacctctt cttaaataag tagttggggt tttttattac aagttcatac
     4081 ctgcccgtcg cttggacttc tgcgacgact ttacacacat cccaccccga gccgtcggcc
     4141 gagtcgtcga agaggacgaa gccggcgctc tcaaggaact tgtcacaatt ctcgaagtct
     4201 cgtttggcaa attcgtatgt gtggttgccg tcgatgtagc agaagcttat ggggccgccg
     4261 agccgcaccg atctccccag aatgtcgctt acctcttccg acctgcgcca cgcgccgaag
     4321 agttcatcag agagcatctc caccgtgtac ggcaagtcgt cacggctaaa catcctgatg
     4381 ttgcgcgtgt aagtctcctt aacgaacgcc ctgtagtcgg cgtggccgat gggagagtcg
     4441 ccgagcgtgc gcccgtcctg cgccccttcg aacgcccact tgtcacttgt gatgagctgg
     4501 ttactcacgc cgcgcttccg cttgtagtag gtcagcaggt tagtggaaag cccgcagaac
     4561 gagccgacct cgacgatcgg cgcccggcta ggtaaattcc tgatggcgtg atcgaagcag
     4621 tagagattcc ccctgtgaag catcccggca ttagcgaaac aaacccagtt tacgtactcg
     4681 tcagatatgt caatgaattg actattaccg gctctggctg cgagttttcg cacaagtctg
     4741 gtagcaaaat ttctcatgat gtccttatgt gatcgcgagc gcctaacgcc caaattaacc
     4801 ggcgagctat caacgttaaa gatgagagcg gcgctgaatg cgagtccggt tgaatgagat
     4861 gttaggccgt tgcctcacca gaggtttccc cgtcgtcttt atcgctaggc ggcgattcaa
     4921 cttgtaaaag gtattgccgc aacccggtct tcagtcgttg ctgcactacc caccccaagt
     4981 ccgtgaatga gaacgctgta tctccatttg aaaactcgcc agcaatacgg atgacttgtt
     5041 tgtcaagaag gcgagaggtc g
//
LOCUS       DASVES010000218         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_430947, whole genome shotgun sequence.
ACCESSION   DASVES010000218 DASVES010000000
VERSION     DASVES010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430947"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..2027)
                     /locus_tag="VF240_08770"
     CDS             complement(63..2027)
                     /locus_tag="VF240_08770"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX7175336.1"
                     /translation="MSPLAGALASAQKTKSKTPVAGSGDAAFKEMHDNYVVNFLRRNP
                     TVNTYLGGAGLDPSLSEADGRMRDHSPEALREEDRWLERALQQFERTDARTLSPARRI
                     DREVAIAQLRFLIHQHQVRRYQERALDTYTDEPFRAIDWQLQGMTQTGPGTYGAIDEW
                     SLVNNRLSLIPEFLMRARTQLLAGVRAGNTPDHRMLLRNGVNTSEANAKYFETTLMGL
                     AEQRVATSFPERAQLLRALRATSNSTARYYREFAAFIRETFFDASASGALTPKPQFRA
                     DRFQMGEREYDWAIRNNLRLDTTAAKLYAESWPIVQATRDEMVALARRVGEKNKWQLP
                     AEGGAAVRAVFDELSKDYPKSDAEMIEWYRAASFRLVDYARKTGVFDVPADYKLEVVE
                     TPPPLQASVDGAAYYPAPPFKQSGVGRFYVTPTGGDEAALRANNRAALADLAAHEGFP
                     GHDWHFKVMTQSREQIAPVRWLTPGAVEDSSSMWQDSMASEGWALYSEALMAEPAAGA
                     PEGFYTPEERLYQLQGKLYRDLRVRIDTGIHTNRMTYDEAVDLFSEVVDFLPGKCSDK
                     TTKSDAKRASCESSERAIFRYSKWPTQAITYRLGKDQIFDLRREAARMAGERFSPKTF
                     HLLFMKQGTIPAGYFREELLRELRERPSPE"
BASE COUNT          345 a          682 c          797 g          399 t
ORIGIN      
        1 gttgaatctc tccgcgcgaa ctccgcgccc tttgcgactc cgcggtgaat ctacttccgc
       61 tatcactcag gtgaaggtcg ctcgcgcagc tcgcgcagaa gctcctcgcg gaagtagccg
      121 gcaggtattg tgccctgctt cataaagagg aggtggaagg tcttgggcga gaatcgttcg
      181 cccgccatgc gcgcggcctc gcggcgcagg tcgaatatct gatctttgcc gaggcgatag
      241 gtgatggcct gcgtgggcca cttcgagtag cggaagatgg cgcgctccga agattcgcag
      301 ctcgcgcgct tggcgtctga cttagtcgtc ttgtccgagc acttgccggg aagaaaatcg
      361 acgacctccg agaagagatc gaccgcctcg tcgtaggtca tccggttggt gtggatgccg
      421 gtgtcgatgc ggacgcggag gtcgcgatag agcttgccct gaagctggta gagccgctcc
      481 tcgggcgtgt agaagccctc gggcgcgccg gccgcgggtt cggccatcag cgcctcggag
      541 tagagcgccc agccctcgga ggccatcgag tcctgccaca tcgaagagga gtcttcaacc
      601 gcgccggggg tgagccaacg cacgggggcg atctgttcgc gcgactgcgt catcaccttg
      661 aagtgccagt cgtggccggg aaagccttcg tgcgcggcga ggtcggcgag cgcggcgcgg
      721 ttgttcgcgc ggagcgcggc ctcgtcgccg cccgtcggcg tgacgtagaa gcgaccgacg
      781 ccgctctgct tgaagggcgg cgccgggtag taggccgcgc cgtcaacgga cgcctgaagg
      841 ggcggcggcg tctcgacgac ttcgagcttg tagtcggccg gcacgtcgaa cactccggtc
      901 ttgcgcgcgt aatcgacgag gcggaacgag gccgccctgt accactcgat catctcggcg
      961 tccgacttcg gatagtcctt cgacagctcg tcgaagacgg cgcgcacggc cgccccaccc
     1021 tccgccggca actgccactt gttcttttcg ccaacgcggc gcgcgagcgc caccatctcg
     1081 tcgcgcgtcg cctgcacaat cggccacgac tccgcgtaga gcttcgcggc cgtggtgtcg
     1141 aggcgcaggt tgttgcggat ggcccagtcg tactcgcgct cgcccatctg gaagcggtcg
     1201 gcgcgaaact gcggcttcgg cgtgagcgcg cccgaggccg aggcgtcgaa gaaggtctcg
     1261 cggatgaagg cggcgaactc gcggtaatac cgggccgtcg aattggacgt ggcgcgcagc
     1321 gcgcggagaa gttgcgcgcg ctcggggaag gacgtggcga cgcgctgctc ggcgagaccc
     1381 atgagcgtcg tctcgaaata cttggcgttg gcctcggagg tgttgacgcc gttgcggagg
     1441 agcatgcgat ggtctggcgt gttgccggcg cggacgccgg cgagcagttg cgtgcgcgcc
     1501 ctcatgagga actcaggtat caggctgagg cggttgttga cgagcgacca ctcgtcgatg
     1561 gcgccgtagg tgccggggcc ggtctgcgtc atgccctgta attgccagtc gatggcgcgg
     1621 aaaggctcgt cggtgtaggt gtcgagcgcg cgctcctgat agcgtctgac ctgatgttgg
     1681 tggatgagga agcggagctg cgcgatggcc acctcgcggt cgatgcggcg ggcgggcgag
     1741 agcgtgcgcg catccgtgcg ctcgaactgc tgaagcgcgc gctccagcca gcgatcctct
     1801 tcgcgcagcg cctccggcga gtggtcgcgc atccggccgt cggcctcgct caaagagggg
     1861 tcgaggccgg ctccgccgag atacgtgttg acggtcgggt tgcgccgcag gaaattgacg
     1921 acgtagttgt cgtgcatctc cttgaacgcg gcgtcgccgc tgccggcgac gggcgtcttc
     1981 gacttcgtct tctgcgcgga cgcgagcgcg ccagccagcg gtgacaagca tgaggcggct
     2041 attaagattg cgagtagaag ttttctcata gtgttctcct gcacattgtc cagtatcgcg
     2101 gcaggcgagc gccgggagtg ggcgcgacct cgcgcacagt accgcgagcg gtagcgagcg
     2161 ggtgcgtctc tggtcaaaca ctcgccgggg tgtgcaaccc gctcgctacc gctcgcggta
     2221 ctg
//
LOCUS       DASVES010000219        16476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1653233, whole genome shotgun sequence.
ACCESSION   DASVES010000219 DASVES010000000
VERSION     DASVES010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16476
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653233"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1638
                     /locus_tag="VF240_08775"
     CDS             91..1638
                     /locus_tag="VF240_08775"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7175337.1"
                     /translation="MIVRDWMSRRAVLSPAKTAVVDAATGAALTYKELDERATRLANY
                     LRDQCRVQSGDRISILSQNRNEVLEAFFAAAKLTAILVPLNHRLTLPELQYILEDCEP
                     RVLIYEREFLPVARRLRRQTAIEHYVALDESEEGNESSVVYEAALSSASGDAVEVEHF
                     EAEAPALIIYTSGTTGRPKGALLSHRMLTWNSINTIIGWDITSDDVTTINAPLFHTGG
                     FNVLTLPLLHVGATVVMMRGFDAEEALRCIEQHRCTIFFGVPTMFQMMLESPRFEATD
                     FSSLRFFISGGAPCPVPLIEAYQKKNVAFTQGYGLTEVGPNCFKLSLEDAVRKAGSIG
                     FPTFHSEARLVDEKGRDVRRGRVGELILKGGHVCSGYWRNAEATRAAMSDCWFYTGDL
                     ARQDEDGYYYIVGRAKDIIISGGENIYPAEVEAVLHSHPSVASAALVGLPDAKWGETP
                     VAAVVARRGSSPTADEILEFCSGRLARYKIPKRVFFVSEFPLLASGKVFKRALREELQ
                     KELSDGG"
     gene            1788..2792
                     /locus_tag="VF240_08780"
     CDS             1788..2792
                     /locus_tag="VF240_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX7175338.1"
                     /translation="MKRTILALSIVTLLLSHASARAAQQGRWVSGRVSNSSGARSYKL
                     WIPAGYAGKNPAPLLLMLHGCTQTPNDFAAGTRMNDIADTNKFLVVYPEQPAEANAYK
                     CWNWFLPEHQTRDAGEPALLAAVVAQLRVSYKVDARRIYVAGVSAGAAMAVNVAAAYP
                     EIFAAVGVNLGLQYKGATNLAEALTAQSKGTGADPNRQGLLAFQAINSVKPGARKRGM
                     PVIVFHGSLDAMVNPANADQVVRQWAQTNDYLDDNADNNTVDDQADATVNGAVSGGHT
                     YTKQVYNDAAGKPLMEKWIVQGMKHAWSGGSAAGSYTDAKGPDASAEMWRFFRSYKLN
                     "
     gene            2856..4280
                     /locus_tag="VF240_08785"
     CDS             2856..4280
                     /locus_tag="VF240_08785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7175339.1"
                     /translation="MNTRRGTIYALSVLFAINALNFFDRQILGAVTEPIRKEWGLSDG
                     AIGLLGTAFTLVYAAVGVPLGRLTDNFRRTRLLAGGVFVWSLLTAVSGLVTNYWQMFA
                     TRLGVGVGEASCAPAATSLIGDLYPPEKRSRALSVFMLGLPVGIALSYAVSGSVAHAY
                     GWRAAFYVAGLPGLLCVIAVLFMREPMRGGMELHAVGAQRREGSPYRLVLTTPTMWWI
                     IASGALQTFNMYAIGSFLTPYLMRYHGLDIRQAGFVTMVVFGLSGIPGLLLGGWAADA
                     IARRRKNGRMLLGTLALLVSAPFILLALMQAREQTLAFILLMGAGVAAMNVYYSTVYP
                     SIHDVTEPSLRGTAMSLYFFAMYVLGAALGPVGTGLASDYFTGRAARAAGVLDTAQTA
                     LEPFRAEGLHSAMYIIPALGLLLTFVLFAGSRTITADMEKLGRWMSGAGARGDSDDAR
                     ARQQDEPLFEHPEETLPPVVKQEP"
     gene            4354..5250
                     /locus_tag="VF240_08790"
     CDS             4354..5250
                     /locus_tag="VF240_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7175340.1"
                     /translation="MELTQLEFFLRVVEEGSFSKAADRVFRTQPAVSIAIRRLEEEIG
                     APLFDRAQKTPTLTDTGEVLYDYARRMLALRDQARDAVAELRELRTGRVRVGANESTS
                     LYLLPDLILAYRAQHPEVKVEIFRQTSERLTREVLDRNVDFALMAFEPTDRDIASFPV
                     LKDELILIMSPKHPLAARDAVTVKDLGSEQFLAHNVKSASRVKVIDTFARHHTPLNIT
                     LELATIETIKRFAQKRIGLAFVPRMCVSEELERGTLSTVPVRGLTYTRTLWATHRRGA
                     THSHAAAAFLELLRRHAKSHPV"
     gene            5372..6607
                     /locus_tag="VF240_08795"
     CDS             5372..6607
                     /locus_tag="VF240_08795"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7175341.1"
                     /translation="MRDVNDNGAVNSRVTIFDTTLRDGEQSPGCSMNLEEKVRLARQL
                     ERLGVDVIEAGFPIASEGDFAAVRAVAAECREVKVAALCRTAEADVRRAWEALRGAAR
                     PRIHTFVATSDIHLEYKLKKTRGEVLEMTRAAVRLARSLAADVEFSAEDATRSDVDYL
                     CEVFAVAVAEGASVLNVPDTVGYTTPTEFRELVSAVRERVVRERDDVIISVHCHNDLG
                     LAVANSLAALDAGARQIECTINGVGERAGNAALEEVVMALHVRADRLPYECGVRTEEI
                     YPSSRLLSETIGFGVQPNKAVVGRNAFAHEAGIHQHGVLSNPLCYEIMTPESVGVPAN
                     RIVLGKHSGRHALGARYGELGYALSRTELDSAYTRFTRLADRKKNIYEQDLISLLSQE
                     TQTRGPLVARAVRFELAGA"
     gene            6713..7831
                     /gene="leuB"
                     /locus_tag="VF240_08800"
     CDS             6713..7831
                     /gene="leuB"
                     /locus_tag="VF240_08800"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX7175342.1"
                     /translation="MKLRITTLPGDGVGPEVTAEAVRVLRAVAEGYDHEFTFDEQLVG
                     GAAIRQTGTPLPRATLDACLASDAVLLGAVGSPEYDELPPAERPEAGLLTLRRALGAY
                     ANLRPVVCHDALADCTPLRPERIAGADILVVRELLGGLYFGEPRGFETADGGSVSAAF
                     NTMRYTVAEVERVARVAFDAARARRRKVTSVDKANVLETSRLWRETVSRVGRDYPTVA
                     LEHVYVDACAMHLIADPLRFDVLLTENLFGDILSDEAAVLAGSLGLLASASIGGEVDL
                     YEPVHGSAPDIAGLGTANPLGAISSAALLLRHTARLEREAQDIERSIENVLSAGYRTP
                     DIARGPESIPVVGTSEMGRLVVEALAEVADMRHAYHAV"
     gene            7917..9320
                     /gene="leuC"
                     /locus_tag="VF240_08805"
     CDS             7917..9320
                     /gene="leuC"
                     /locus_tag="VF240_08805"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF004016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7175343.1"
                     /translation="MSTLFDKLWNAHVVCDPSDGPALLYIDLHLVHEVTSPQAFEGLR
                     EAGRRVRRPDLTVATIDHNVPTTNRALPIADPIASQQMETLRRNCREFGVKLYDIDSA
                     EQGIVHVIGPELGLTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQSLPQT
                     RPRTMLIKVRGQLSAGVTAKDLALAVIGQIGTAGATGHCIEYAGEAVRGLSMEGRMTL
                     CNMSIEAGARAGMIAPDETTFEYLAGRPFAPKGEAWDEALRYWRTLQSDEGAEFDSTI
                     ELDASLVEPHVTWGTSPGMVAPVTGRVPLPAEAASASDAVAFERALSYMSLRPGTRIE
                     EIGVDRVFIGSCTNARLEDLRAAAKVVKGYRVSGGVSAMVVPGSQAVKRAAEAEGLDG
                     VFREAGFEWREAGCSMCLGMNPDTLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAA
                     AAAVTGHFTDVREWKYK"
     gene            9411..10070
                     /gene="leuD"
                     /locus_tag="VF240_08810"
     CDS             9411..10070
                     /gene="leuD"
                     /locus_tag="VF240_08810"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7175344.1"
                     /translation="MIPFRTHNGLVAPLDRPNVDTDQIIPKQFLKRVERTGFGQFLFY
                     DWRFTAAGEPDSSFVLNEPRYAGASVLVAGRNFGCGSSREHAPWALLDYGFRAIIAPS
                     FADIFANNCMKNGIVTVELPEELVAELTRRVRDAEGYRVTVDLERCEVRDGDGFLTRF
                     EMDEFRRRCLLEGLDETGLTLLREEDIAAFEARRPRWLDMNARLSAGRSSLTNSGART
                     Q"
     gene            10067..10213
                     /locus_tag="VF240_08815"
     CDS             10067..10213
                     /locus_tag="VF240_08815"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX7175345.1"
                     /translation="MILRVSGERELLENNPALARSIHLRYPDIDPMSFIRVELPRHKR
                     EDEA"
     gene            10234..12090
                     /locus_tag="VF240_08820"
     CDS             10234..12090
                     /locus_tag="VF240_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7175346.1"
                     /translation="MKVSEEKKKRPRASDAWREARDLIWAHRQRLALGMALMLVNRLV
                     GLVLPASSKFLIDEVITKGRAELLTTIALAAGAATLLQALTSFALSQVLGVAAQRAIT
                     EMRKSVQAHIARLPVRFFDSTQTGALISRIMSDAEGIRNLVGTGLVQLVGGVVTAAIS
                     LCVLFYLNWRLTSITILVLAVFGGVMALAFSRLRPLFRERGEINAEVTGRLGESLGGI
                     RIVKAYSAERRETLVFARGAHRLFRNVARSMTGVSGVTAASTLIVGAIGVVMILVGGR
                     SIIAGTMTLGDFFMYIFFTALVAAPMVSIAAIGTQITEAFAGLDRIREIMRVTTEDEE
                     DASRQPLANVAGEIAFDDVSFEYDAQSPVLRNVSFRAPAGTTTALVGSSGSGKSTLIG
                     LVMAFNRPLAGTVTVDGRDLSTVRLRDYRQQLGAVLQDNFLFDGTVADNIGFANPHAT
                     RAEIEEAGRVAHCEEFVRGFPEGYDTIVGERGVKLSGGQRQRVAIARAILSGPRILIL
                     DEATSSLDSESEIMIQDGLEKLRSGRTTFVIAHRLSTIRSADQILVLEAGRVVERGTH
                     EELLAAAGRYRELYDKQYNFERNRFINPGEDFTPEPPKTSPPDSAQVTSNAL"
     gene            12198..12635
                     /locus_tag="VF240_08825"
     CDS             12198..12635
                     /locus_tag="VF240_08825"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HEX7175347.1"
                     /translation="MAGTPQTFANHTRWHAPFHFFVVPVMGLNVVWSIVQLVRHPGWD
                     RAEWALVSLALVVLTALVRLNPLRAQDRLIRLEEQLRYQRLLPAALAARCAGLSPGQI
                     VALRFASDAELEGLVSRVLGGEISKPRDIKKAIREWRPDTFRV"
     gene            complement(12791..13255)
                     /locus_tag="VF240_08830"
     CDS             complement(12791..13255)
                     /locus_tag="VF240_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7175348.1"
                     /translation="MHPNEELIENFYTSFQKLDAEAMTRCYHPDIRFSDPVFPVLSGA
                     EVGAMWRMLCSQAKNFELTFADVEANDRVGKARWEARYDFSATGRRVHNKIEAEFEFR
                     DGKIIKHTDTFDFWKWSRMALGPAGVVLGWTPLLRRKVQKQAGERLAGFMKS"
     gene            complement(13289..14095)
                     /locus_tag="VF240_08835"
     CDS             complement(13289..14095)
                     /locus_tag="VF240_08835"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7175349.1"
                     /translation="MSSVHTQPVGNLLRDWRRRRRLSQLDLACEAEISSRHLSFLETG
                     RSSPSREMVLRLAEQLEIPLRERNRLLVAAGYAPIFKERPLGDPTLEAARKVVELVLA
                     GHEPYPALAVDRHWNLIASNRAVTPLLAGVEPSLLETPVNVLRLSLHPRGLAPRIVNL
                     AEWRSHLLARLRRQIDASADPVLGELLNELSAYPISSENKYNAADGIDYAGVAVPFQF
                     ATEGGVLSFLSTTTVFGTPVDITLSELALESFFPADAATSDALNRSLKRE"
     gene            14252..14653
                     /locus_tag="VF240_08840"
     CDS             14252..14653
                     /locus_tag="VF240_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175350.1"
                     /translation="MQLFRTSTFLRWVLFADAATCIAAGLLLMLGSRVLEQFLGLPAE
                     LSRYAGVSLLPFAALLVYLATRENLSSPVVWAVIALNALWTVDSILLLLSGWIEPTGL
                     GSAFIVFQAFGVAMFAALEYLGLRKSAIAAA"
     gene            14944..15336
                     /locus_tag="VF240_08845"
     CDS             14944..15336
                     /locus_tag="VF240_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175351.1"
                     /translation="MNKARILVVEDQHFMRIGIIALLSGAGYEVAEAQNGAEGLELAT
                     PFRPDAVTIDYRLPDTTGLEFAETLRGQPGFASTPFILVTSEQMPGDCDSTPLPHITA
                     YLRKQDLIEHLLPCLASHLRGEAYSPAS"
     gene            complement(15380..>16476)
                     /locus_tag="VF240_08850"
     CDS             complement(15380..>16476)
                     /locus_tag="VF240_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175352.1"
                     /translation="EAQAEGAPFTFALLELQSLRGGGVELARRIRADAKLDGTRLVLI
                     TSSVLGHDEEIEYERLFDAFLTMPMRRLQLKDHLLALVNRDQRHGVATPPGGDARPSA
                     SPGGGRTILVAEDNSVNQMLMTHLLEELGFGAVVVSNGVSALDACARGEYVMVLMDCQ
                     MPEMDGYAATAELRRREGAGRHTPVIAMTADAMPGTRERCLAAGMDDYLSKPFTREQL
                     EDLIAKWDGRRVVRAAESTSPEEDAPVDEVLDLSLIERLHGFRERGDVGALARLIDTF
                     LQDINARRDAISEAYGSRDREKVRREAHALKGSAGFFGAVRLSKVCAELIDPATGDDL
                     PIAREVLKRLDDELIYVTRKLEAWRCIHPA"
BASE COUNT         2888 a         5506 c         5180 g         2902 t
ORIGIN      
        1 gataagtgat gagtgataag tgataagaga aagcatcttc ttatctcatc actcatcact
       61 catcactcat cactctgccc ggaggttgag atgatcgtca gagactggat gtcgcgccgc
      121 gccgtgctct cgccggcgaa gacggcggtc gtagacgcgg cgacgggcgc ggctctgacc
      181 tacaaagaac tcgacgagcg cgccacgcgc ctcgccaact acctgcgcga ccagtgcagg
      241 gtacagagcg gcgaccgcat cagtatcctt tcgcaaaacc ggaacgaggt gctggaagcc
      301 ttcttcgcgg cggccaagct gacagccatc ctcgtcccgc tcaaccaccg cctcacgctc
      361 cccgaactgc aatacatcct cgaagactgc gagccgcgcg tgctcatcta cgagcgcgag
      421 tttctcccgg tcgcccgccg cctgcgccgg cagacggcca tcgagcatta cgtggcgctc
      481 gacgagtccg aagagggtaa cgagtcttct gtcgtctacg aggcggcgct ctcatcagcc
      541 agcggcgacg cagtggaggt cgagcacttc gaagcggaag cgcccgcgct catcatctac
      601 acttcgggca cgacggggag gccgaagggc gcgctgctct cgcaccgcat gctgacgtgg
      661 aactccatca acacgattat cggctgggac atcacgtccg atgacgtaac gaccatcaac
      721 gcgccgctct tccacacggg cggcttcaac gtgctcacgc tcccgctcct gcatgtcggc
      781 gcgacggtcg tcatgatgcg tggcttcgac gccgaagagg cgcttcgctg catcgagcaa
      841 caccgctgca cgatcttctt cggcgtgccg acgatgttcc agatgatgct tgagtcgccg
      901 cgcttcgagg cgaccgattt ctcgtcgctc cgcttcttca tctcgggcgg cgcgccgtgc
      961 ccggtgcctt tgattgaggc ttaccagaaa aagaacgtgg ccttcaccca aggctacggc
     1021 ctgaccgaag tcggcccgaa ttgcttcaag ctctcgctcg aagacgccgt gcgcaaggcc
     1081 ggctcaatcg gcttcccgac ttttcatagc gaggcgcgtc tcgtcgatga gaaggggcgc
     1141 gacgtgcggc gcggccgagt aggcgagctg atcctcaagg gcggccacgt ctgctccggc
     1201 tactggcgca acgccgaggc cacgcgcgcg gcgatgtccg actgctggtt ctacacgggc
     1261 gatctggcgc gtcaggacga ggacggctac tactacatcg tcggtcgcgc gaaagacatc
     1321 atcatctcgg gcggcgagaa catctatccg gcggaggtcg aggccgtcct gcattcgcac
     1381 ccgtcggtgg cctcggccgc cctcgtcgga ctgcccgatg cgaagtgggg cgaaacgccc
     1441 gtcgcggcgg tcgtcgcacg ccggggcagc tctcctaccg ccgacgagat tcttgagttt
     1501 tgttcgggcc gactcgcgcg ctataagata ccgaagcgcg tcttcttcgt cagcgaattt
     1561 cccctgctcg cctcgggtaa ggtattcaag cgggcgctca gggaagagct tcagaaggaa
     1621 ctctcggacg gcgggtgaga gcatgggcga cgccggacag taccgcgagc ggtagcgagc
     1681 gggtgtgcct gtgctatagg ctcgcctcgc acacacaccc gctcgctacc gctcgcggta
     1741 ctgttcccgc agcactcgca cactgactat aactatctga gatgaccatg aaacgcacaa
     1801 tacttgctct ctccatcgtg acgcttttgc tgtcacacgc gagcgcgcgg gcggcgcagc
     1861 aggggagatg ggtctctggc cgcgtcagca actcctccgg cgcgcgcagc tacaagctct
     1921 ggattccggc gggctacgcc ggaaaaaatc ccgccccgct cctgctgatg ctgcatggct
     1981 gcacgcagac cccgaatgat ttcgccgccg gcacgcgcat gaacgacatc gccgacacaa
     2041 ataaattcct cgtcgtctac cccgaacagc cggcggaagc gaacgcctac aagtgctgga
     2101 actggtttct tcccgagcac cagacgcgcg acgcgggcga gccggcgctg ctcgcggcgg
     2161 ttgtcgcgca gctacgagtc tcctacaagg ttgacgcgcg ccgcatctac gtcgccggcg
     2221 tctcggcggg cgcggcgatg gcggtcaacg tggccgcggc ctaccccgaa atcttcgccg
     2281 ccgtcggcgt caacttgggt ctgcaataca agggcgcgac caatctcgcc gaagccctca
     2341 ccgcgcaatc caagggcaca ggagcagacc ccaaccggca aggtctgctc gcctttcagg
     2401 caatcaacag cgtgaagccc ggcgcacgca agcggggcat gcccgtgata gtcttccacg
     2461 gctcgctcga cgcgatggtc aacccggcca acgccgatca agtcgtcaga cagtgggcgc
     2521 agaccaacga ctatctcgac gacaacgccg ataacaacac cgtggacgat caggcggacg
     2581 cgacggtgaa cggcgccgtc agcggcggcc acacgtacac gaagcaagtc tataacgacg
     2641 ccgcagggaa gcccctcatg gagaagtgga tcgttcaggg tatgaagcac gcgtggtcgg
     2701 gaggctccgc ggccggctcc tacacggacg ccaaaggccc ggacgcgagc gccgagatgt
     2761 ggcgcttctt ccgttcatac aaacttaact aggccgcgca tggagcgcag cggggcgaag
     2821 cagtgaggcc gagccgctct cgtgaaggct tgaagatgaa cactcgcagg ggaacgattt
     2881 acgcgctctc ggttctgttc gcgatcaacg cgctgaactt cttcgaccgg cagatactcg
     2941 gcgcggtgac cgaacccatc cgcaaggagt ggggactgag cgacggcgcc atcggcttgc
     3001 tcggcacggc gttcacgctc gtctacgcgg cggtcggcgt ccccctgggc cggctcacgg
     3061 acaacttccg gcgcacgcgc ctgctcgcgg gcggcgtgtt cgtctggagc ctgctcacgg
     3121 ccgtgtcggg actcgtgacg aactactggc agatgttcgc cacgcgcctc ggcgtcggag
     3181 tgggtgaggc gagctgcgcg cccgccgcga cctctctgat cggcgacctc tacccgccgg
     3241 agaagcgttc gcgcgcgctc tcggttttca tgttgggcct ccccgtcggc atcgccctca
     3301 gctacgccgt cagcggttcg gtggcgcacg cctacgggtg gcgcgcggcc ttctacgtcg
     3361 cgggactgcc cggcctcctc tgcgtcatcg ccgtcctctt catgcgcgag ccgatgcgcg
     3421 gcgggatgga gttgcacgcc gtcggcgcgc aacgccgcga agggtcgcct tatcgtctgg
     3481 tgctgacgac gccgacgatg tggtggatca tcgcatcggg ggctttgcag accttcaaca
     3541 tgtacgccat cggctctttc ctcacgccgt acctgatgcg ctatcacggc ctcgacatcc
     3601 gccaggccgg cttcgtgacg atggtcgtct tcgggctatc gggcatcccc ggtctcctgt
     3661 tgggaggctg ggcggccgac gccatcgcgc ggcgtcgcaa gaacgggcgg atgctcctcg
     3721 gcacgctcgc tctgctcgtt tccgcgccgt tcatcctcct cgcgctcatg caggcgcgcg
     3781 agcaaacgct cgccttcatc ctcctgatgg gcgcgggcgt ggcggcgatg aacgtctatt
     3841 actcgaccgt ttatccgtcc attcacgacg tgaccgagcc gtctttgcgc ggcacggcga
     3901 tgtcgctcta cttcttcgcc atgtacgtcc tcggcgcggc cctcggcccc gtcgggacag
     3961 ggctggcgag cgactacttc acggggcggg cggcgcgggc ggcgggcgtc ctcgacacgg
     4021 cgcagacggc gctcgaaccc ttccgcgccg aggggctgca ctcggccatg tacatcatcc
     4081 ccgcgctcgg actcctgctc acgtttgtcc tcttcgccgg ctcgcgcacg atcacggctg
     4141 acatggagaa gcttggccgt tggatgagcg gcgcgggcgc gcgcggcgat tcggacgacg
     4201 cgcgcgcgcg ccaacaggac gagccgctgt tcgagcaccc ggaagagact ttaccgccgg
     4261 tcgtcaaaca agagccgtga gcgcgcgaga taattccgcc ggggaatcgg tgttatattc
     4321 ctcgccgctt agttcacgac ggacggacgc gcgatggaat taacgcagtt ggaattcttc
     4381 ctgcgggttg tcgaggaagg cagcttttcg aaggccgccg accgcgtctt ccgcactcag
     4441 ccggcggtga gcatcgccat ccgccggctc gaagaggaga tcggcgcgcc gctcttcgac
     4501 cgcgcgcaaa aaaccccgac gctgacggac acgggcgaag tcctctacga ctacgcgcgg
     4561 cgcatgctcg cgctccgcga tcaggcgcgc gacgccgtcg cggaactgcg cgagttgcgg
     4621 acgggccgcg tccgcgtcgg cgcgaacgaa agcacctcgc tctacctcct gcccgatctc
     4681 atcctcgcct accgcgcgca gcacccggag gtgaaagtcg aaatcttccg gcagacctca
     4741 gagcgtctga cgcgcgaagt gctcgaccgc aacgttgact tcgcgctgat ggccttcgag
     4801 ccgaccgacc gcgacatcgc ttctttcccc gtcctcaaag acgagctgat tctcatcatg
     4861 agtccgaagc acccgctcgc ggcccgcgac gcggtgaccg tcaaagacct cggcagtgag
     4921 cagttcctcg cacacaacgt caagtcggcc tcgcgcgtga aggtgatcga cactttcgcc
     4981 cgacatcaca cgccgctcaa catcactctg gagctggcga ccatcgagac catcaagcgc
     5041 ttcgcgcaga agcgcatcgg cctcgccttc gtcccgcgca tgtgcgtgag cgaggagctt
     5101 gagcgcggca cgctctccac cgtccccgtg cgcggcctga cctacacgcg cacgctctgg
     5161 gccacgcacc gacgcggcgc cactcactcg cacgcggcgg ccgccttcct cgaactcctt
     5221 cgccgccacg cgaagtcgca ccccgtctga agtcctgatc cccggccccg gccccgtatc
     5281 actcagattt atccgcctat aaaaactata actttgactt aatgcgcgga tgctctctag
     5341 gatggccgct tgcccgagga cggaggcccc tatgagagac gtaaacgaca acggcgcagt
     5401 caatagtcgc gtcacgattt tcgacacgac gctcagggac ggcgagcagt cgcccggctg
     5461 ctcgatgaat cttgaggaga aggtcaggct cgcgcggcag ctcgaacgcc tcggcgttga
     5521 cgtgatcgag gccggattcc ccatcgcctc cgaaggcgac ttcgcggcag tccgcgccgt
     5581 ggcggcggag tgccgcgaag tgaaagtggc cgccctctgc cgaaccgcgg aggccgacgt
     5641 gcggcgcgcg tgggaggcgc ttcgcggcgc ggcccgcccg cgtattcaca cgttcgtcgc
     5701 gacctccgac atccatctcg aatacaaatt gaagaagacg cgcggcgaag tgttggagat
     5761 gacgcgcgcc gccgtccgcc tcgcccgctc gctcgccgcc gacgtggagt tctccgccga
     5821 ggacgcgacg cgcagtgacg ttgattatct ctgcgaggtc ttcgccgtgg cggtcgcaga
     5881 gggggcgagc gtcttgaacg tccccgacac ggtcggctac acgacgccga cggagttccg
     5941 cgagctggtc agcgccgtgc gcgagcgcgt cgtccgggag cgcgacgacg tgattatcag
     6001 cgtccactgc cacaacgacc tcggactcgc cgtcgccaac agcctcgccg cgctcgacgc
     6061 gggcgcgcgg cagatcgaat gcacgatcaa cggcgtcggc gagcgcgccg gcaacgccgc
     6121 gctcgaagag gtcgtgatgg ccttgcacgt gcgcgccgac cgcctgccct acgagtgcgg
     6181 tgtccgcacc gaggagattt acccttcgag ccgcctgctc tccgagacca tcggcttcgg
     6241 cgtgcagccg aacaaggccg tggtgggccg caacgccttc gcgcacgagg cgggcatcca
     6301 ccagcacggc gtgctctcga acccgctctg ctacgagatc atgacgccgg agtccgtggg
     6361 cgttcccgcg aaccgcatcg tgctcggcaa gcactcgggc cgccacgcgc tcggcgcgcg
     6421 ctacggagag ttgggctacg cgctgtcgcg cacggagctg gattcggcgt atacgcggtt
     6481 cacgcgcctc gccgaccgca agaagaacat ctacgaacag gatttgatct ccctgctttc
     6541 gcaggagacg cagacgcgcg ggccgctcgt cgcgcgggcc gtcaggttcg agttagccgg
     6601 cgcgtagatg gggtgggcta ccgcgcgggc atagccgcgg gccgctcatg cacgttaagg
     6661 taatgagcgg ctcgcggcta tttttgtata agagaatgtg ggtgaccaga ggatgaagtt
     6721 gagaatcacg acgctgcccg gcgacggcgt cggcccggaa gtgacggcgg aggcggtgcg
     6781 cgtgctgcgc gccgtggccg aagggtacga ccacgaattc accttcgacg aacaactcgt
     6841 cggcggcgcg gccatacgcc aaacggggac gccgctcccg cgcgcgacgc tcgatgcgtg
     6901 cctcgcgtct gacgccgtgc tgctcggcgc ggtcggctcg ccggagtatg acgagttgcc
     6961 gccggccgaa cggcccgaag ccgggctgct cacgctgcgg cgcgcgctcg gcgcatacgc
     7021 gaacctgcgc cccgtcgtct gccacgacgc gctcgccgac tgcacgccgc tgcggcccga
     7081 gagaatcgcg ggcgcggaca ttctcgtcgt gcgcgaactt ctcggcggcc tctacttcgg
     7141 cgagccgcga ggctttgaga ccgccgacgg aggcagtgtg agcgcggcct tcaacacgat
     7201 gcgctacacg gtcgccgagg tcgagcgcgt cgcccgcgtc gccttcgacg ccgcccgcgc
     7261 gcgccgccgc aaggtgacct ccgttgacaa ggccaacgtg cttgagacct cgcgactctg
     7321 gcgcgagacc gtctcgcgcg tcgggcgcga ctacccgacg gtcgcgctcg aacacgtcta
     7381 tgttgacgcc tgcgcgatgc acctgattgc cgacccgctg cgcttcgacg tgctgctcac
     7441 cgaaaatctc ttcggcgaca tcctctcgga cgaggccgcg gtgctcgccg gctcgctcgg
     7501 cctcctcgct tcggcgagca tcggcggcga agttgacctc tacgagccgg tgcacggctc
     7561 cgcgcccgac atcgccggac tcggcacggc caacccgctc ggcgcaatct cgtccgccgc
     7621 gctgctgctc cgccacacgg cgcggctcga acgcgaggcg caggacatcg aaaggtcaat
     7681 cgaaaacgtg ctgagcgccg gataccgcac gcccgacatc gcgcgcgggc cggagagcat
     7741 tccggtcgtc ggcacgtccg agatgggccg gctcgtcgtc gaggcgctgg ccgaagtcgc
     7801 cgacatgcgc cacgcgtatc atgcagtttg aaaacagagg tcagaggtca gaggttagag
     7861 gtcagttgaa aactgtcttc actgacctct gacctctgac ctctgacctc tttcttatgt
     7921 caacactgtt cgacaagttg tggaacgctc acgtcgtctg tgacccttcg gacgggcctg
     7981 cgttgcttta cattgatctg cacctcgttc acgaggtcac ttcgccgcag gcgttcgagg
     8041 gcttgcgcga agcgggccgg cgtgtgcgcc gccccgatct gacagtcgcc acgattgatc
     8101 acaacgtccc cacgacaaat cgtgcgctcc ccatcgccga ccccatcgcc tcccagcaga
     8161 tggagacgct caggcgcaac tgccgcgagt tcggcgtgaa gctttacgac attgattcgg
     8221 cggagcaggg catcgtccac gtcatcggcc cggaactggg cctgacgctt cccgggatga
     8281 cgattgtgtg cggcgacagc cacacctcca cgcacggcgc gttcggcgcg ctcgccttcg
     8341 gcatcggaac gtccgaggtc gagcacgtgc tcgccacgca gtcgctcccc cagacgaggc
     8401 cgcgcacgat gcttatcaaa gtgcgcggcc aactgagcgc gggcgtgacg gcgaaagacc
     8461 tcgcgctcgc cgtcatcggc cagataggga cggccggcgc gacggggcac tgcatcgagt
     8521 atgccggaga ggccgtgcgc ggtctttcga tggagggccg catgacgctc tgcaacatga
     8581 gcatcgaggc gggcgcgcgc gccgggatga tcgcgcccga cgagacgacc ttcgagtatc
     8641 tcgccggccg accgttcgcg ccgaagggcg aggcttggga cgaagcgctt cgctactggc
     8701 gcacgcttca aagcgacgag ggcgcggagt tcgacagcac gattgagctt gacgcgtccc
     8761 ttgtcgagcc gcacgtgacg tgggggacga gtcccggcat ggtcgcgccc gtgacggggc
     8821 gcgtgccctt gcccgcggag gcggcgagcg cgtcggacgc cgtcgccttc gagcgcgctc
     8881 tgagttacat gagcctgcgg cccggcacgc gcatcgagga gatcggagtt gaccgtgtct
     8941 tcatcggctc atgcacgaac gcgcgcctcg aagacctgcg cgccgccgcg aaggtggtca
     9001 agggctaccg cgtgagcggc ggcgtgagcg cgatggtcgt gcccggctcg caggcggtca
     9061 agcgcgcggc ggaagctgaa gggttagacg gcgtctttcg cgaggccggc ttcgagtggc
     9121 gcgaggccgg atgctcgatg tgcctcggca tgaatcccga cacgctcaac cccggcgagc
     9181 gctgcgcttc gaccagcaac cgcaacttcg agggccgtca ggggcgcggc ggccgcacgc
     9241 acctcgtcag ccccgcgatg gccgccgccg ccgccgtcac gggacacttc acggacgttc
     9301 gcgagtggaa atacaaatag aaagtgatga gtgatgagtg ataagtgata agaaagagcc
     9361 gctgtctttt cttatcactt atcacttatt actcatcact tatcactctt atgattccct
     9421 tccgcacaca caacgggttg gtcgccccgc tcgacaggcc gaacgttgac accgaccaga
     9481 tcatccccaa gcagttcctc aagcgcgtcg agcgcacggg gttcggacag tttctcttct
     9541 acgactggcg cttcacggcg gcgggtgagc ctgactcgtc cttcgtgctg aacgagccgc
     9601 gctacgcggg cgcgagcgtg ctcgtggcgg gccgcaactt cggctgcggc tcttcgcgcg
     9661 agcacgctcc gtgggcgctc ctcgactacg gcttccgcgc catcatcgcc ccgtcgttcg
     9721 ccgacatctt cgccaacaac tgtatgaaga acggcatcgt gaccgtcgag ctgccggagg
     9781 agttggtggc ggagttgacg cggcgagtgc gcgacgccga aggctaccgc gtgacggttg
     9841 acctcgaacg ctgcgaggtg cgcgacggag atggattcct gacgcgcttc gagatggacg
     9901 agttccggcg tcgttgtctg ctcgaaggtc tcgacgagac ggggctaacg cttctgcgcg
     9961 aagaagacat cgccgccttt gaggcgcgcc gcccgcgctg gctcgacatg aatgcgcgcc
    10021 tcagcgctgg gagatcgtcg ttgacgaatt cgggcgcacg cacgcaatga tcttgcgcgt
    10081 cagcggtgag cgggaattac tggagaacaa tcctgcgctc gcccgctcaa tccatttacg
    10141 ctaccctgat atcgatccga tgagcttcat tcgggtcgag ctgccgcgtc ataagcgcga
    10201 agacgaggcg tgaagcggcg gccagagggg ggcgtgaaag tgtcggagga aaagaagaag
    10261 cgcccgcgcg cgtcggacgc atggcgcgaa gcgagagacc tcatctgggc gcatcgccaa
    10321 cggctcgcgc tggggatggc gctgatgctc gtcaaccggc tcgtcgggct ggtgctgccc
    10381 gccagctcca agttcctcat cgacgaggtc atcacgaagg ggcgcgccga actgctgacg
    10441 acgattgcgc tcgcggcggg cgcggcgacg ctcttgcagg cgttgacttc gtttgcgctc
    10501 tcgcaggtgc tcggtgtcgc ggcgcagcgc gccatcaccg agatgcgtaa gagcgtgcag
    10561 gctcacatcg cgcgcctccc cgtccgcttc ttcgactcca cacagaccgg cgcgctcatc
    10621 tcgcgcatca tgtcggacgc cgagggcatc cgcaatctcg ttggcacggg gctggtgcaa
    10681 ctcgttggcg gggtggtgac ggcggccatc tcgctctgcg tcctcttcta cctcaactgg
    10741 cgtctgacct cgattacgat cctcgtgctc gcggtcttcg gcggcgtcat ggcgctggcc
    10801 ttctcgcgcc tgcggcccct gttccgcgag cgcggcgaga tcaacgccga ggtgacgggc
    10861 cgcctcggcg aatcactggg cggcatccgc atcgtcaagg cttactcggc ggagcggcgc
    10921 gagacgctcg tcttcgcgcg cggcgcgcac cgcctgttcc gcaacgtcgc ccgctcgatg
    10981 acgggcgtct ccggcgtcac ggccgcctcg acgctcatcg tcggggccat cggcgtcgtc
    11041 atgatcttag tcggcggccg ctcgatcatc gcggggacga tgacgctcgg cgacttcttc
    11101 atgtacatct tcttcacggc gctcgtggcc gcgccgatgg tctcgatagc cgccatcggg
    11161 acgcagatta cggaagcctt cgccggcctc gaccgcatcc gcgagatcat gcgcgtcacg
    11221 accgaagacg aggaggacgc gagccgccag ccgctcgcca acgtcgcggg cgagatcgcc
    11281 ttcgacgacg tgagcttcga gtacgacgcg cagtcgcccg tccttagaaa cgtctctttc
    11341 cgcgccccgg ccgggacgac gaccgcgctc gtcggctcca gcggctcggg caagagcacg
    11401 ctcatcggcc tcgtcatggc cttcaaccgc ccgctcgcag gaaccgtcac cgttgacggg
    11461 cgcgacctct cgaccgtgcg gctgcgcgac taccgccagc aactcggcgc cgtcctgcaa
    11521 gacaacttcc tcttcgacgg cacggtcgcc gacaacatcg gcttcgccaa cccgcacgcc
    11581 acgcgcgccg agatcgaaga ggcgggccgc gtcgcccact gcgaggagtt cgtccgcggc
    11641 ttccccgaag gctacgacac catcgtcggc gagcgcggcg taaagctctc cggcgggcag
    11701 cggcagcgcg tggcgattgc gcgggcgatt ctctcggggc cgagaatcct gatcctcgac
    11761 gaagcgactt cgagcctcga cagcgaaagc gaaatcatga ttcaggacgg actggagaaa
    11821 ctccgcagcg gccgcaccac gttcgtcatc gcgcaccgac tttcgaccat ccgcagcgcc
    11881 gatcagattc tcgtgctcga agcgggccgc gtcgtcgagc gcggcacgca cgaagaactg
    11941 ctcgcggccg ccgggcgtta ccgagagctt tacgacaagc agtacaactt cgagcgcaac
    12001 cgcttcatca accccggcga agacttcacg cccgagccac ccaaaacttc gcccccggac
    12061 tccgcgcaag tcacttccaa cgcgctctga cgaaagggcg tgcgcttcgg cggcgcgcgt
    12121 cggacgcgtt cgacgcggtt cgagacgaag agtgattcat ccgaatccgt gacggatatt
    12181 tcagaggaga aaaaaagatg gcgggaactc cccaaacttt cgcgaaccac acgcgctggc
    12241 acgccccttt tcattttttc gtggtgccgg tgatgggctt aaacgtcgtc tggtcaatcg
    12301 tccaactcgt gaggcatccg ggctgggatc gtgccgagtg ggcgctcgtc tcgctcgcgc
    12361 tcgtcgtgtt gaccgcgctc gtgcgcctca acccgctgcg cgcgcaggac agactcatcc
    12421 ggctcgaaga acaactccgc tatcagaggc ttctgccagc cgcgctggcc gcgcgttgcg
    12481 ccggactgtc gcccggacaa atcgtcgcct tgcgtttcgc ctccgacgcc gagctggaag
    12541 gactcgtcag ccgcgtgctc ggcggagaga tttccaaacc acgcgacatc aaaaaggcga
    12601 tcagggagtg gcgccccgac acgttccgtg tttgaacgcc gggcagttag aggaagaggg
    12661 aagtgacggc ggtggataat gctgtcggaa aagtcgcgga ggttgttcga tctcaatcag
    12721 ataaccatcg gggactcggc cgaaacatcg ccgctcgaat tgtgcgcggc gcgcgtctgc
    12781 atcgaaacac ctacgacttc ataaatcctg ctaaacgctc acccgcctgt ttttgaactt
    12841 tcctgcgaag caacggcgtc caacctaaga caactccggc cgggcctaaa gccatccggc
    12901 tccacttcca aaaatcgaag gtgtccgtgt gtttgataat cttcccatcc cgaaactcga
    12961 actcggcctc aatcttgtta tgaactcgcc gccccgtcgc ggaaaaatca tatcgagctt
    13021 cccaacgagc cttccccacc cgatcattcg cctcgacatc ggcaaaggtg agttcaaagt
    13081 ttttggcttg tgaacataac atccgccaca tggcgccaac ttccgcgccg gacagaacgg
    13141 ggaagaccgg gtcggaaaaa cggatgtcgg gatgataaca acgcgtcatc gcctcggcgt
    13201 ccagtttttg aaaggacgtg tagaagtttt cgatcagctc ttcgttcggg tgcattcgtc
    13261 gtctctccct ccagaaaact cgtgcggttc attcccgctt caaacttcga ttcaaagcgt
    13321 ccgacgtggc ggcgtcggcc gggaagaagg actccagcgc cagctccgac agggtgatgt
    13381 caacaggcgt tccgaagacg gtcgtcgtgc tgagaaaaga gagaacgccg ccttccgtcg
    13441 cgaactgaaa aggcacggcc acgccggcgt aatcaatgcc gtccgcggcg ttgtatttgt
    13501 tttcactcga tattggatac gcgctcagtt cattcaataa ctctcccagc accgggtcgg
    13561 cgctcgcgtc aatctggcgg cgcagacgcg cgagcaggtg cgaacgccac tcggccagat
    13621 taactatccg gggcgcgagt cctcgcggat gcaggctgag acgaaggacg ttgaccggcg
    13681 tctccagcag agacggctcc accccggcga gtaaaggcgt gacggcgcgg ttcgaggcga
    13741 tcagattcca atgacgatcc acggcgagcg ccgggtacgg ctcgtgtccg gccagcacta
    13801 actccacaac tttgcgcgcc gcctcaagcg tcgggtctcc gagcggcctc tccttgaaga
    13861 tcggcgcgta tccagccgcg accagcagcc ggttgcgctc gcgcaagggg atttcgagtt
    13921 gttcggcgag acgcagaacc atctcgcggc tcgggctgga acgccccgtt tcgagaaagc
    13981 tcaggtgcct gctcgaaatc tcggcttcac aggcgaggtc gagctggctc aagcgtctgc
    14041 gcctgcgcca gtcgcgcagc agattcccga cgggttgagt gtgaacactg ctcatcaagc
    14101 cccgacggta tcacaaatcg tgaaggcggc tccattacct ccgaagtaat tgttttctaa
    14161 acacaagtcc gcaatgatgc gggcggacaa ggggaacgga tggataaccg tcacggctca
    14221 cggctcaatt cacttttgga ggttcaatgg tatgcagtta tttcgcactt cgacttttct
    14281 tcgctgggtg ctgttcgctg acgcggcgac ctgcatcgcc gccggattgc tgctgatgct
    14341 cggctccaga gttcttgaac agttcctcgg gctgccggcg gagctatcgc gctacgcggg
    14401 cgtcagcctg ctgcccttcg cggcgctgct cgtctatctc gccacgcggg agaatctttc
    14461 gtcgccggtg gtctgggcgg tcatcgcact caacgcgctc tggacggtgg acagcattct
    14521 gctcttgctg agcggatgga ttgagccgac cggactgggc agcgccttca tcgtctttca
    14581 ggctttcggt gtggcgatgt ttgccgcttt ggagtatctc ggcttgagga agtcggcgat
    14641 agcggctgcc tgaggttacg gccgggggct ttcgaggaga aggtaacggc gcgacggggc
    14701 gaggctgatt aacagtcagc ctcgccccgt cgcgcacgaa atcaacctgt attttctgta
    14761 acggctgttc atctcgttcc gcgatctccg gcgcggtcgc gtcgctttcc taatcccacc
    14821 tgaactttcg cgtgtggttg gcgtgacgga cgtgacatga atcgcccgga taagctatgc
    14881 tttgccggct gcggttagtt tcaaatcctc gcatcgcctc attgtttccc accggagcac
    14941 gttgtgaata aagccagaat cctcgtcgtc gaggatcagc acttcatgcg aatcggcatc
    15001 atcgccctcc tcagcggcgc cggttacgag gtcgccgagg cacaaaacgg cgcggagggc
    15061 ctcgaactgg cgacgccgtt caggcccgac gccgtgacta tcgattaccg cctccccgat
    15121 actaccgggc ttgaattcgc cgagacgtta cgcggtcagc cgggatttgc atcgaccccc
    15181 ttcatcctcg tcacctcgga gcaaatgccc ggcgactgcg actccacgcc gctcccgcac
    15241 atcaccgcct acctgcgtaa gcaagacttg atcgaacacc tgttgccgtg cctcgcatca
    15301 catctccgcg gggaagcata ttcgcccgcg tcttaagacc gcccggcccc cggcgcacgc
    15361 gccgacccca ccagtctcgc taggcggggt gaatacatct ccacgcttcc agcttgcgcg
    15421 tgacgtaaat cagttcgtcg tcaaggcgct tcaacacttc acgggcgata ggcaaatcgt
    15481 cgccggtggc cgggtcaatc agttcggcgc aaaccttcga caggcggacg gccccgaaaa
    15541 aaccggcact ccccttcagc gcatgggcct cgcgccgcac cttctcccgg tcgcgggagc
    15601 cgtaggcctc gctgatggca tcgcggcgcg cgttgatgtc ctgaaggaaa gtgtcgatga
    15661 gccgggcgag ggcgccgaca tcgccccgct cccggaagcc gtgcaggcgc tctatcaggc
    15721 tcaggtcgag cacttcgtca acaggggcgt cctcttcagg ggaggtggac tcggcagccc
    15781 gcacgacgcg gcggccgtcc cacttcgcga tcaagtcctc tagttgctcg cgcgtgaagg
    15841 gcttgctgag gtagtcgtcc atgcctgccg cgagacaccg ctcgcgcgtc ccgggcatgg
    15901 cgtcggccgt catggcgatc accggcgtgt gccgtcccgc gccctcgcgg cgccgcagtt
    15961 cggcggtcgc cgcgtagccg tccatctccg gcatctggca gtccatcaac accatcacgt
    16021 actcgccgcg ggcgcacgcg tcgagcgcgc tcacgccgtt cgacacgacc accgcgccga
    16081 accccaactc ttcgagcagg tgagtcatga gcatctggtt caccgagttg tcttcggcca
    16141 ccagaatagt ccggcctccg ccgggggacg cgctcggccg ggcgtcacca cccggcggcg
    16201 tggcgacccc gtggcgctga tccctgttaa cgagcgcgag taggtgatct ttgagttgca
    16261 ggcgccgcat gggcatcgtg aggaaggcgt cgaagagtct ttcgtattca atctcctcat
    16321 cgtgcccgag gacggaggag gtaatgagga cgagccgcgt gccgtcaagt ttggcgtcgg
    16381 cccgaatccg cctcgccaac tcaacccccc cgccgcgcaa gctctggagt tccaacagcg
    16441 cgaaggtgaa gggcgcgccc tcggcctgcg cctcgc
//
LOCUS       DASVES010000220         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1591097, whole genome shotgun sequence.
ACCESSION   DASVES010000220 DASVES010000000
VERSION     DASVES010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591097"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..969
                     /locus_tag="VF240_08855"
     CDS             157..969
                     /locus_tag="VF240_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175353.1"
                     /translation="MSFTGSYSRRMRRAALTALAAVCFATVATRAQGGQQSWSKPLEE
                     WTREDAQKVLNASPWARRQSIKVEQRRTGRIAGAPNPIIDGNQAPLQTEANTATSGGA
                     QAPIDYTFTLRLRSALPVRQALTRLMQLDAPKLKEKDRAAHETRIKGTLACPACADNY
                     VVSLSSRSEESQGSDPVYAAFAGARIDDIKRFIYLANDRGDRRALVHFVPPRSPGEEA
                     VFFFPRLDERGEPLLTPASRKLIFNVTKVEVSTVANFQIDVADITNNGEVIF"
     gene            1030..3327
                     /locus_tag="VF240_08860"
     CDS             1030..3327
                     /locus_tag="VF240_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175354.1"
                     /translation="MKDYPRNERLIPRDDAGPRAARRGRALLIVPALLLLCGLVVVSG
                     RANGGATEGNAEDKKTGAEYEKMVAKSYDYRFGPNPFAPSNATSATGAFIPGAKFLPS
                     ARCATCHTDAHDQWRQSAHGNAFREPFYQKNVLDIIAQKDIAFTRHCESCHNPAALFS
                     GALTKKPSFKARPFDEDGVSCIACHSIQEVNGRGIGGYTMGEPALLVKEDGARLLEGV
                     TDQQILDDVPSHRRAMMRPVLKSNEFCGACHKSQVPKELNDYKFLRAFAVADELQMSS
                     FSKESPHPFYVRDKESCNSCHMKKEPAPRFDVSAKEGTITSHRWAAANTAIPFFYGYQ
                     AQLDAVRKFLEADVLGIDIFALRRDPAGREGTELFAPLNRSDFQIAAGDTLTADVVVT
                     NKNIGHSFPPELRDFYEAYVEFTVADGEGRPVYRSGFVKPDGYLDESAHTYKTYLVMA
                     DGSFNDKHHIWRTRVVAQNNQIQSGRSDLARYKFTVPATVAGGRLRMTARVQYRRFTR
                     VFQTYALGKEVDYPNVLMAATEQTLLVGENKQTPLPPAGAKGVMPDWRRWNNYGISLL
                     DQRQFALAADAFARAADLDTAYRPMARVNRALALIELDRWNDAAKLVDAALEEKPDLY
                     RALFQRGRIRERRGELKEAEEDYRRVSEAFPRDRLTLQRLGELAKIKRDFVAASAFYE
                     RILQIDPEDAGSHYNLMLVYRKLGRQEEARREAKIFADLKDDPGALPVASEFLRRHPE
                     MKGESVPWHVHDLDRGRESAAYTKN"
     gene            3351..>3733
                     /locus_tag="VF240_08865"
     CDS             3351..>3733
                     /locus_tag="VF240_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7175355.1"
                     /translation="MPAGVTGLAVKCAVLCAFVIMTLGYALAAAPREAATAQAKSGAR
                     RASAQKTQARGREVYISNCARCHGGDGRGQTTLGLSVEAPDMTDARWHARRGARRMTQ
                     SVARGRGQMPAFAGKLTKAEIAATIA"
BASE COUNT          689 a         1306 c         1180 g          558 t
ORIGIN      
        1 tggctgacga caaggtgcgg acgcagcaga tgctcggcca gtcgccgcgc taagacgggg
       61 acggcgtggt tcgtggccgc gcgcttggag cgaagaggcc gcgaaggccg cgagccggaa
      121 ggtgttcgag agttctacaa aagggaagta agaggcatga gttttaccgg aagttattcg
      181 agacgaatgc gccgcgccgc cctcacggcc ctcgcggcag tgtgtttcgc gacggtcgcg
      241 acgcgcgcgc aaggggggca gcagtcgtgg tcgaagccgc ttgaggagtg gacgcgcgag
      301 gacgcgcaga aagttttgaa cgcttcaccg tgggcgcgca gacagtcgat caaagttgag
      361 cagcgcagga ccggccgcat cgccggcgcg ccgaacccga tcatcgacgg caatcaggcc
      421 ccgctccaga ccgaggccaa cacggccacg agcggcggcg cgcaggctcc tatcgactat
      481 accttcacgc tgcgcctgcg ctcggcgctc cccgtccggc aggcgctcac gcgcctcatg
      541 cagctcgacg ccccgaagct caaggagaaa gaccgcgccg cccacgagac gcgtatcaag
      601 gggacgctcg cctgcccggc gtgcgccgac aactacgtcg tgtcgctcag ttcgcgctct
      661 gaggagtcgc aaggctccga tcccgtctac gcggccttcg ccggcgcgcg catcgacgac
      721 atcaagcggt tcatctatct ggccaacgac cgcggcgacc gccgcgcgct cgtccacttc
      781 gtgccgccgc gctcgcccgg cgaggaggcc gtcttcttct tcccgcgtct cgacgagcgc
      841 ggcgagccgc tgctcacccc ggcgagcagg aagctcatct tcaacgtcac gaaggttgag
      901 gtcagcacgg tcgccaactt tcaaatcgac gtggcggaca tcacgaacaa tggggaagtc
      961 attttctgaa gggacgcaca cttccgccgc gcgccctcgg cttcggctta atgaaaaggt
     1021 gttgaaaaga tgaaagacta cccccgaaac gaacggctca ttccgcgcga cgacgccggc
     1081 ccgcgcgcgg cgcggcgcgg acgcgcgctc ctgatcgtcc cggccctgct tctgctgtgc
     1141 ggcctcgtcg tcgtgagcgg ccgggcaaac ggcggagcga cggaaggaaa cgcggaggac
     1201 aagaagacgg gcgccgagta cgagaagatg gtcgccaagt cttacgacta ccgcttcggc
     1261 ccgaacccgt tcgcgccctc gaacgccact tccgccacgg gcgccttcat ccccggcgcg
     1321 aagttcctcc cctcggcgcg ctgcgccacc tgccacacgg acgcgcacga ccagtggcgg
     1381 cagtcggccc acggcaacgc cttccgcgag ccgttctacc agaagaacgt tttggacatc
     1441 atcgcgcaga aggacatcgc ctttacacgc cactgcgaat cgtgtcacaa cccggcggcg
     1501 ctgttctccg gcgcgctcac caagaaacct tccttcaagg cgcggccctt cgacgaggac
     1561 ggcgtctcct gcatcgcctg ccactccatt caggaagtga acggccgcgg catcggcggc
     1621 tacacgatgg gcgagccggc actactcgtt aaggaagacg gcgcgcgtct tctggaaggc
     1681 gtcaccgacc agcagatact cgacgacgtg ccctcgcacc gccgcgcgat gatgcgcccg
     1741 gttctgaagt ccaacgagtt ctgcggcgcg tgccacaagt cgcaggtgcc gaaggagctg
     1801 aacgactaca agttcctgcg cgccttcgcc gtcgccgacg agttgcagat gtcttcgttc
     1861 tcgaaggagt cgccgcaccc gttctacgtg cgcgacaagg agtcgtgcaa ctcctgtcac
     1921 atgaagaaag agcctgcgcc gcgcttcgac gtgtcggcca aagagggcac tatcacctcg
     1981 caccgctggg ccgccgccaa caccgccatc ccgtttttct acggctatca ggcgcagctc
     2041 gacgccgtcc gcaagttcct cgaagcggac gtgctcggca tcgacatctt cgccctgcgc
     2101 cgcgaccccg cgggccggga gggaaccgag ctgttcgccc cgcttaaccg cagcgacttc
     2161 cagatagcgg cgggcgacac tttgacggcc gacgtggtcg tcaccaacaa gaacatcggc
     2221 cactcgttcc cgcccgagct gcgcgacttc tacgaggcct acgtcgagtt caccgtggcc
     2281 gacggcgagg gccgccccgt ctaccgctcc ggcttcgtca agcccgacgg ctacctggac
     2341 gaatcggccc acacctacaa gacctatctc gtgatggccg acggctcctt caacgacaag
     2401 caccacatct ggcgcacgcg cgtcgtcgcg cagaacaatc agattcagtc gggccgctca
     2461 gaccttgccc gctacaagtt caccgtgccg gcaactgtcg ccggcggccg tctccgcatg
     2521 acggcgcgcg tgcagtatcg tcgcttcaca cgcgtcttcc agacctacgc gctcggcaag
     2581 gaagttgatt accccaacgt cttgatggcc gcgaccgagc agacgttact tgtcggcgag
     2641 aacaagcaga cgccgctccc gccagccggc gcgaagggcg tcatgccaga ctggcgccgg
     2701 tggaacaact acggcatctc gctcctcgac cagcgccagt tcgcgctcgc cgccgacgcc
     2761 ttcgcccgcg ccgccgacct tgacacggcc taccgcccga tggcgagggt caaccgcgcg
     2821 ctcgcgctca tcgagcttga ccgctggaat gatgcggcga agctcgtcga tgcggcgctc
     2881 gaagagaagc ccgacctcta tcgcgcgctc ttccagcgcg ggcgcatccg cgagcgtcgc
     2941 ggcgagctga aggaagcgga agaggattat cgccgcgtct cggaagcctt cccgcgcgac
     3001 cgcctgacgc tccagcgtct cggcgagctt gcgaagatca aacgcgactt cgtcgccgcc
     3061 agcgccttct acgagcgcat cctccagatc gaccccgagg acgccggctc gcattacaac
     3121 ctgatgctcg tttatcgcaa gctcgggcgg caggaggagg cgcggcgcga ggccaaaatc
     3181 ttcgccgacc tcaaggacga ccccggcgcg ctgcccgtgg cgagtgagtt tctgcgccgc
     3241 catccagaga tgaagggcga gagcgtgccg tggcacgtcc acgacctcga ccgcgggcgc
     3301 gagtcggccg cctacacgaa aaactgaagg acaaggggac aagccaaggg atgccagcag
     3361 gtgttacggg cctcgccgtg aagtgcgccg ttctatgcgc ctttgtgatt atgactctcg
     3421 gctacgcact cgccgccgcc ccgagggaag cggcgacggc gcaggccaaa agcggcgcgc
     3481 gccgggcatc cgcgcagaag acgcaggccc gcggacgtga ggtatacatc tcaaactgcg
     3541 cgcgctgtca cggcggcgac ggccgggggc agaccacgct cggcctctcg gttgaggcgc
     3601 ccgacatgac ggacgcgcgc tggcacgcgc gccgcggcgc gcgtcgcatg acgcagtcgg
     3661 tcgcgcgcgg acgcgggcag atgccggcct tcgccggcaa gctcacgaag gctgagatcg
     3721 ccgcgaccat cgc
//
LOCUS       DASVES010000221         9811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1649771, whole genome shotgun sequence.
ACCESSION   DASVES010000221 DASVES010000000
VERSION     DASVES010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649771"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VF240_08870"
     CDS             complement(<1..527)
                     /locus_tag="VF240_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175356.1"
                     /translation="MRTFDLVIIFGYLAGITLFGVWFSRKQKTTKDYFLGDRSVPWWA
                     IAASIVATETSTITFISVPGVAFSRGGNFQFLQLVMGYMLGRVVISFLFIPSYFRGEL
                     LTVYQLLDRRFGGKIKMLAAALFVVMRNIADGIRLLLTALVLAAVYTAFVPGADVQSI
                     VVASVVLLGAVMIVFT"
     gene            complement(524..1042)
                     /locus_tag="VF240_08875"
     CDS             complement(524..1042)
                     /locus_tag="VF240_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175357.1"
                     /translation="MESFQLPRKKWPLALCCAACLALELALYFGVTRPALSAMRAGLG
                     LEGEWSWVSIAGWHAAVALGCAALAWQPLAELRTKFTNEGVRRPRLFAPALFVGWDEA
                     ESVFVAPSLESPQHVKIKAPRGSVVINALFYKNPQRLLSLIEERMRAHTTSSAPACPA
                     RAADFETATTHK"
     gene            complement(1214..2155)
                     /gene="murQ"
                     /locus_tag="VF240_08880"
     CDS             complement(1214..2155)
                     /gene="murQ"
                     /locus_tag="VF240_08880"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX7175358.1"
                     /translation="MSEDLHTNIPVTEQENPRTRDISSQPATEIVRLMNEEDARVAEA
                     VRLVLPEVARAVEGIVERLGRGGRLFYTGTGTSGRLGVLDASECPPTFGVAPELVQGI
                     IAGGYEACYRAVEASEDDAEAGARDIDARGVGPEDVLVGIAASGRTPYTVGALAHARS
                     RGTLTVALTCAPDSPITRAADIAIVPEVGPEVIAGSSRLKAGTAQKLVLNMLSTASMI
                     RLGYVTGNRMTNLLPRNTKLRARAVRNLAAETGLGEEEARAAIEAAGGDPRTALVMTK
                     AGASRDEAERALVESKGAVARAVELVAAARDSRGREG"
     gene            complement(2166..3152)
                     /locus_tag="VF240_08885"
     CDS             complement(2166..3152)
                     /locus_tag="VF240_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7175359.1"
                     /translation="MKPRSDPRRLAAAKRLRRRELVLGVDGGGTKTRAVITNAAEEVL
                     GEGLAGPSNPLRVGVVPAALAVRVAVERACAAARVRRGDILAAQVGLAGSRRSDLRER
                     MREALAGLGINPLEVVTDADIALFGATEGKPGLIIIAGTGSICCGVNGRGKRVCAGGW
                     GPLAGDEGGGSWIARRALQAVAKGADGRGPVTSLDAAACDYFNVAKSDDIALAIYNPS
                     MTNQVIAGFTRRVVGEAARGDSVARSIVEEAGRELGVAAVAVIRGLRMRRERFQVSYV
                     GGVFAAGEIIFKPLTEEIARVAPGAFLAPPLIAPAEAAARMAREHLRLALAG"
     gene            3420..4400
                     /locus_tag="VF240_08890"
     CDS             3420..4400
                     /locus_tag="VF240_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7175360.1"
                     /translation="MDIRQLKAFAAIAEARTFTAAAVRVHVTQAAISMQIRQLETEIG
                     VPLFLRTPRRVVLTEAGERLLDRAHAILREHDAALAEMAELAGAERGRLRLGSASAMV
                     SADPLPAILKELRETHQQAEVSVVTGTSESLVKQILAGDLDMAFVSLPVEARNIETEL
                     LSTDQLVAIASPQHKMAERRVVSAFALAGEKLILGERGGNTRRLIDEFFAESGLKPTV
                     AMELSRLAAIRKMVESDMGVGIVPLRSVLEEVADGRLVRWWIEGAHVNWELGVARLSG
                     GYLSPVCDTFLKLCRKHFEPGPDAAGPRNLSRRKKADDSSAPTRKSRKKR"
     gene            complement(4695..5072)
                     /locus_tag="VF240_08895"
     CDS             complement(4695..5072)
                     /locus_tag="VF240_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175361.1"
                     /translation="MVEVSRPAILFVEDISDTRELVEFSLRQDGFDIKTVQTAQEGLD
                     LARKKSYALILLDIGLPDKDGLQLCREIREFDPQTPIVLYTAYGELLDEAEAARAGAQ
                     GCLRKPDDTARLSEALRGYIDKA"
     gene            complement(5087..5623)
                     /locus_tag="VF240_08900"
     CDS             complement(5087..5623)
                     /locus_tag="VF240_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX7175362.1"
                     /translation="MNQSLESRAARVKLLLMDCDGVLTDGRITLLDGGGEQKSFHTRD
                     GHGIVMLHRAGLRTGVISGRTSTALDARARDLGMHFVRQGTWDKVKDYEELLAEAGVR
                     EEEVGYVGDDVTDIPLMRRCGFAAAVADATEDTRAAAHYVTRLNGGFGAVREVCELLL
                     KAQGRWSELLERYTGVEG"
     gene            complement(5750..8056)
                     /locus_tag="VF240_08905"
     CDS             complement(5750..8056)
                     /locus_tag="VF240_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175363.1"
                     /translation="MKKLRILLLLFIPLLLAQDVWAQADARSAWQVIRYDITANVGGL
                     SATERALAVRATINATNVGGAAGRTLTARVNPAAIVSAATVGGAPAKFTTETDGQTKL
                     LMVRVALPEAVPPGGATSVQIDYRLTVSENNGLATLSAEGAQLLPLSHWYPTPNTPIA
                     PRGADYAPFRLTVEGASGEGAGVVSAGRATGATHEVALSAQPFFLTGKWETIEGSAEA
                     RGVSALVPPGASADERKRAESLIALAGAARSFYAGLLGPAPDTPIRLVAVRRGSGFDM
                     AGAILLDAAVFRRAKTDAVTTMNIAEGVARLWVGGATAVQGEGAGVVREGLPRFLATH
                     FIEKQFGQPAADTERARMAQLYSAVALRDAPLSQSSPAYDTYYTSVANKGALVWRLLM
                     ERIVGRENFMAALRREFDPAKGARVSLAALRASLGERASDASKAAFEALFDRPTDTDL
                     LVGKPMQEAGEWRAVLRNLGSIDTDVNVAATNERGERLTTRVNVPARGDATARFKTAA
                     PVVFVEADPERLYPQLDFAKDVAPWWPTAAEEMLSDARAALSAQPARAESVSREVLAR
                     RPLMQEARVLLGRALLAQNKTAEAEREFRAALDLPLPTPYTLAWANIGLGEVALKKGQ
                     PAEGARHFDEAVRADAEYAATLAARAARIRAETAAGSTPAVDEAARAAVAQLDAAIRS
                     GKKAELDNIIMAGELSDFSKGIVGSQPELWQTRLLRTEQMGANRVAADVEIKARTLGA
                     DQAGTAVLVFARTPAGWKLADVQLFEVR"
     gene            complement(8235..9074)
                     /gene="kdsA"
                     /locus_tag="VF240_08910"
     CDS             complement(8235..9074)
                     /gene="kdsA"
                     /locus_tag="VF240_08910"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX7175364.1"
                     /translation="MNIKPFRVGDATFGDGRLTLIAGPCVVESREHVLMMARECAKRA
                     LDAGLDYVFKASFDKANRSSHESFRGIGVEEALDALRAVKEETGLPVLTDVHDVAQAQ
                     LAAGVADVLQIPAFLCRQTDLIVAAAETGRAVNVKKGQFLSPWDARNIVEKARAAGCE
                     RLLLTERGASFGYNNLVVDMRTFPVMRSFGVPVVFDVTHSLQLPGGLGRATGGQSEYI
                     EPLARAGVACGVDAVFMEVHDAPERALSDGPNALPLARLAALLKTLRDLHELVRREAA
                     VES"
     gene            complement(9092..>9811)
                     /locus_tag="VF240_08915"
     CDS             complement(9092..>9811)
                     /locus_tag="VF240_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175365.1"
                     /translation="VSEPRSAAEFYERGAYYFSSRNFDGAVRDYRRALELQPDFPEAH
                     NRLGRVLMMQGKLGEATEEFRRAVEQKQGNYPAAQYNLGFALQARGDLDEAIEAYDGA
                     VKSRGGTYPDAFFQIGLAHLNRRRYREAADALARAAEQNNGRDADAHNQRGFALAMLE
                     DYTGAESAFRAAIDERGGDFAEAHFRLGMMYAETGRTAEAAKELEIYIQQEPNAENRR
                     RGERVLRELRRKLENEGGQPR"
BASE COUNT         1576 a         3330 c         3315 g         1590 t
ORIGIN      
        1 gtgaagacga tcatcaccgc gccgagcagc acgaccgagg cgacgacgat tgattgcacg
       61 tccgcgcccg gcacgaaggc cgtgtagacg gcggcgagga cgagcgccgt caagagcagc
      121 cggatgccgt cggcgatgtt ccgcatgacg acgaagagcg cggcggcgag catcttgatc
      181 ttgccgccga aacgccggtc gagcagttga tagacggtta gaagttcgcc gcgaaagtag
      241 gacgggatga agaggaagga gatgacgacg cgccccagca tgtagcccat gacgagctgg
      301 agaaactgga agttgccgcc gcgcgagaag gcgacgccgg gcacggagat gaacgtgatg
      361 gtcgaagtct cggtggcgac gatggaggcg gcaatcgccc accacggcac cgagcggtcg
      421 ccgaggaagt agtccttcgt cgtcttctgc ttgcgcgaga accacacgcc gaagagcgtg
      481 atgccggcga ggtagccaaa gatgatgacg aggtcgaatg ttctcacttg tgtgtggtcg
      541 ctgtctcgaa gtcggcggcg cgcgcggggc acgcgggcgc ggaagaagtt gtgtgcgccc
      601 tcatccgttc ctcgatgagt gacaagaggc gctgcggatt cttgtagaag agcgcattga
      661 tcacgacgct gcccctcggc gctttaatct tcacatgctg cggactctcc aacgagggcg
      721 cgacgaagac gctctcggcc tcgtcccacc cgacgaagag cgcgggcgcg aacagacgcg
      781 gacgccggac gccttcgttc gtaaacttcg tccgcaactc ggcgagcggc tgccacgcga
      841 gcgccgcgca cccgagcgcc acggccgcgt gccaaccagc gatgctcacc cagctccact
      901 caccttcgag accgaggccg gcgcgcattg ccgacagggc cgggcgcgtc acgccgaagt
      961 agagcgctaa ctccagcgcg agacaggcgg cgcagcagag cgcgaggggc cacttcttgc
     1021 ggggcagttg gaacgactcc atgagtcctg ttgacgagcc gaggcagatt acgaacgaat
     1081 cagtgtacgg gagacgcgtt gacagtaccg ctagcggaag cctcgcgggt gtggttacga
     1141 cagcagcacc cgcgaggctt ccgctcgcgg tactgttcat tacacccaac cgacgcgtgc
     1201 ggagaatata gcatcaaccc tcgcgcccgc gtgagtcgcg cgccgccgcg accaactcga
     1261 cggcgcgagc aaccgcgccc ttcgattcga cgagcgcgcg ctccgcctcg tcgcgcgacg
     1321 cccccgcctt tgtcatcacg agcgccgtgc gcgggtcgcc gcccgcggcc tcgattgcgg
     1381 cgcgcgcctc ctcctcgcca agccccgtct ccgccgcgag atttcgcacg gcgcgcgcgc
     1441 gcagcttcgt gttgcgcggc aggaggttgg tcatgcggtt gccggtcacg tagccgagcc
     1501 ggatcatcga cgcggtcgag agcatgttga ggacgagctt ctgcgccgtg cccgccttca
     1561 gacgcgacga cccggcgatg acttcggggc cgacttcggg gacgattgcg atgtcggccg
     1621 cgcgcgtgat cggtgagtcg ggcgcacagg tcagggcgac ggttaacgtc ccgcgcgagc
     1681 gcgcgtgtgc gagcgccccg acggtgtaag gcgtgcgacc cgaagcggcg atgccgacga
     1741 gcacgtcttc agggccgacg ccgcgggcgt cgatgtcgcg tgcgccggcc tcggcgtcgt
     1801 cctccgaggc ttcgacggcg cggtaacacg cctcgtagcc gccggcgatg atgccctgca
     1861 cgagttcggg cgcgacgccg aaggtcggtg gacactccga ggcgtcgagc acgccgaggc
     1921 ggcccgacgt gcccgtgccg gtgtagaaga gtcggccgcc gcgtcccagt cgctccacga
     1981 ttccctcgac ggcccgcgcc acctcgggca gcaccagccg cacggcctcc gccacgcgcg
     2041 cgtcctcctc gttcatcagc cgcacgatct ccgtggccgg ctggctgctg atgtcgcgcg
     2101 tgcgcgggtt ctcctgctcc gtgacgggga tgttcgtgtg gaggtcttcg gacattgtta
     2161 aagtgtcagc cggcgagagc gagacggagg tgttcgcggg ccatgcgcgc ggcggcctcc
     2221 gcgggcgcga tcagtggcgg cgcgaggaac gcgcccggcg cgacgcgcgc gatctcctcc
     2281 gtgagcggct tgaagatgat ctccccggcg gcgaagacgc ccccgacgta actcacctga
     2341 aaacgctcgc gccgcatccg caggccgcgg atgacggcca ccgccgccac gccgagttcc
     2401 cttcccgctt cctcgacgat tgagcgcgcc acactgtcgc cgcgcgcagc ctccccgacg
     2461 acccggcgcg tgaatcccgc aatgacctga ttcgtcatcg aagggttgta gatggccagc
     2521 gcgatgtcgt ccgatttggc gacgttgaag tagtcgcagg cggccgcgtc gagtgaggtc
     2581 acggggccgc gcccgtcggc ccccttcgcc acggcctgca aggcgcggcg ggcgatccac
     2641 gagccgccgc cctcatcgcc cgcgagcggc ccccaaccgc cggcgcacac gcgtttgccc
     2701 cgtccgttca caccgcagca gatcgacccc gtgccggcga tgatgatcag acccggcttg
     2761 ccctcggtcg ccccgaacag agcgatgtcg gcgtcggtca cgacttcgag cggattgatg
     2821 ccgagtcccg caagcgcctc gcgcatgcgc tcgcgcaagt ctgaacgcct cgacccggcg
     2881 aggcccacct gcgcggccag aatgtcgccg cgccgcacgc gcgccgccgc gcacgcccgc
     2941 tccacggcca cacgcacggc cagcgccgcc ggcaccacgc ccacacgcag agggttcgac
     3001 ggcccggcca gaccctcgcc cagaacttct tcggccgcgt tcgtgatgac ggcccgcgtc
     3061 ttcgtgccgc cgccgtcaac gccgagcacg agttcgcgcc ggcgcaaacg cttcgcggcg
     3121 gcaagtctac gggggtcgct gcggggcttc atcggagttt caggtgtcgg cggccacttg
     3181 agttcttcgg ggacgaccgc gcaattaggt gtttaattgt aaagaccttc cgggtataat
     3241 tgaaactaaa agattttatt cgggcgataa ccgtcgctaa taccaagagg ctaaagccgt
     3301 aaatcgtgaa tcgtcaatcg tgaatcgaaa aaggcgacgc ctctgggatt tacgatttgc
     3361 cattcaagcc gaaggaagcg caaccacgat ttacgattca cgattcacga ttcacgacta
     3421 tggacattcg ccaactcaaa gccttcgccg ccatcgccga ggcccgcacc tttaccgcgg
     3481 cggccgtccg cgtacacgtc acgcaggcgg ccatcagcat gcagatccgt cagctcgaaa
     3541 ccgagatcgg cgtgccgctc tttctccgca cgccgcgccg agtagtgctg acggaagccg
     3601 gcgagcgtct gctcgaccgc gcccacgcca tcctgcgtga gcacgacgcg gcgctcgccg
     3661 aaatggccga actcgccggg gccgagcgcg ggcgtttgcg tctgggcagc gcctccgcga
     3721 tggtcagcgc cgacccgctg ccggccatcc tcaaggagct gcgcgagacg caccagcagg
     3781 ccgaggtgtc ggtcgtgacg gggacgagcg agtcgctggt gaaacagata ctggcgggcg
     3841 acctcgatat ggctttcgtc tccctgcccg tggaggcgcg caacatcgag acggagctgt
     3901 tgagcacgga tcagttggtg gccatcgcca gcccgcaaca caagatggcc gagcggcgcg
     3961 tggtgagcgc gtttgcgctc gcgggcgaga aactgatttt gggcgagcgc gggggcaaca
     4021 cgcgccggct catcgacgag tttttcgcgg agtcggggct aaagccgacg gtggcgatgg
     4081 agttgagccg gctagcggcg atcaggaaga tggtggagtc ggacatgggc gtcggcatcg
     4141 tccctttgcg ctcggtgctc gaagaggttg ccgacggccg gctcgtgcgc tggtggatcg
     4201 agggcgcgca cgtcaactgg gaactcgggg tcgcccgcct gagcggcggc tatctctcac
     4261 ccgtctgcga caccttcctc aaactctgcc gcaaacactt cgagccgggg ccagacgccg
     4321 ccgggccgcg caacttgtcg cgccggaaaa aggctgacga ctcttcagcc cccacgcgga
     4381 agtcgcggaa gaagcgttga cgcgtaacgt tatttgaggc tcggaagatt tcaattgtgc
     4441 tggcgcgcgt cgccagcgcc ggaggcgcga cagaatgtag cccacggcgt cagccgtggg
     4501 gacacgcgcc tgtcagaatt caaagccccg gaggggcgaa agaatctgtg cgggcgtaat
     4561 gctcaagccc gattttcttt cgcccctccg gggctttcga catttataac ggatgcttca
     4621 cccacggctg acgccgtggg ctacattctg ccgccgccgc tgcggctcat agccgcgccg
     4681 ccgcgcgctg acgactaggc tttatcgatg tagccgcgca gcgcctcgct gaggcgggcc
     4741 gtgtcgtcgg gcttgcgtag acagccttgc gcgccggcgc gggcggcctc cgcttcgtcg
     4801 agcagctcgc cgtaggcggt gtagagcacg atgggggttt gcgggtcgaa ctcgcgtatc
     4861 tcgcggcaga gctgaagccc gtctttgtcg ggtagcccga tgtcgagcag gatgagcgca
     4921 tacgacttct tgcgcgcgag gtcgaggccc tcctgggccg tctgcacggt cttgatgtcg
     4981 aacccgtcct gccgcaggct gaactcgacc aactcgcgcg tgtccgagat gtcttcgacg
     5041 aacaggatcg ccggccggct cacttcaacc atcagtctcc cctcccttac ccttcgactc
     5101 ctgtgtacct ttccaacaac tccgaccagc gaccctgcgc ctttaacaac aactcgcaga
     5161 cctcgcgcac cgccccgaag ccgccattca ggcgcgtgac gtagtgcgcg gcggcgcgcg
     5221 tgtcttcggt ggcgtcggcg acggccgccg cgaagccgca acggcgcatc aacgggatgt
     5281 cggtcacgtc gtcgcccacg tagccgacct cttcttcccg gacgccggcc tccgcgagca
     5341 gttcctcgta gtccttcacc ttatcccatg tgccctgtcg gacgaagtgc atgccgaggt
     5401 cgcgtgcgcg cgcgtctaac gccgtcgagg tgcggcccga gatgacgccg gtgcgcaggc
     5461 ccgcgcggtg cagcatgacg atgccgtggc cgtcgcgtgt gtggaagctc ttctgttcgc
     5521 cgccgccgtc gaggagcgtg atgcgcccgt ccgtcaacac gccgtcgcaa tccatgagca
     5581 ggagcttgac gcgcgcggcg cgactctcaa gtgattggtt cattggctca ttagttgcca
     5641 tcacacgcac agtaccgcga gcggtagcga gcgggtgcgg ctgtgatgag agactcgaca
     5701 cgtgcacgcg agacccgctc gctaccgctc gcggtactgt tctcattcat catcgcactt
     5761 caaacaactg tacgtcggcc agcttccagc cggccggcgt gcgcgcgaag acgaggacgg
     5821 ccgtgcccgc ctgatcggcg ccgagcgtgc gcgcttttat ctccacgtcg gcggcgacgc
     5881 ggttcgcgcc catctgttcg gtgcgtagca gccgcgtctg ccacaactcg ggctggctgc
     5941 cgacgatgcc tttcgagaag tcggacagct cgcccgccat gatgatgttg tcgagttcgg
     6001 ccttcttgcc gctgcggatc gcggcgtcga gttgcgcgac ggcggcgcgc gccgcctcgt
     6061 ccacggccgg agtggagccg gcggcagtct cggcacggat gcgcgcggcg cgcgcggcga
     6121 gtgtggcggc atactcggcg tcggcgcgca cggcctcgtc gaagtggcgc gctccttcgg
     6181 cgggctggcc cttcttgagg gcgacctcgc cgagtccgat gttcgcccac gcgagcgtgt
     6241 agggggtggg gagcggcagg tcgagcgcgg cgcgaaactc gcgctcggcc tcggccgtct
     6301 tgttctgcgc gagcagggcg cggcccaaga ggacgcgcgc ctcctgcatc agcggccggc
     6361 gcgcgagcac ttcgcgcgag accgactcgg cgcgcgccgg ctgcgcggaa agtgcggcgc
     6421 gcgcgtccga caacatctcc tcggccgcgg tgggccacca cggcgccacg tccttggcga
     6481 agtcgagctg cgggtagaga cgttcggggt cggcctcgac gaagacgacg ggcgcggccg
     6541 ttttgaagcg cgccgtggcg tcgccgcgcg ccgggacgtt gacgcgcgtc gtcagacgct
     6601 caccgcgttc gttcgtcgcc gccacgttca cgtccgtgtc aatcgagccg agattgcgca
     6661 gcaccgcgcg ccactcgccg gcctcctgca tgggcttgcc cacgagcagg tcggtgtcgg
     6721 tcggtcggtc aaacagcgcc tcgaaggccg ccttcgacgc gtcgctcgcc cgctcgccca
     6781 gtgacgcgcg cagcgcggcg agactcacgc gagcgccctt cgcggggtcg aactcgcgcc
     6841 gcagcgccgc catgaagttt tcgcggccca cgatgcgctc catcaacaga cgccagacga
     6901 gcgcgccctt gttggcgacg gaagtgtagt aagtgtcgta ggccgggctt gactgtgaga
     6961 gcggcgcgtc gcgcagggcg acggccgaat agagctgcgc catgcgcgcg cgctccgtgt
     7021 cggccgcagg ttggccgaac tgtttttcga tgaagtgcgt ggcgaggaag cgtggcaggc
     7081 cctcgcgcac gacgcctgcc ccctcgccct gcacggccgt cgcgccgccc acccacagac
     7141 gcgccacgcc ctcggcgatg ttcatcgtcg tgacggcgtc ggttttagcg cgccggaaga
     7201 cggcggcgtc gagcaggatg gcgcccgcca tatcgaagcc tgagccgcgg cgcacggcga
     7261 cgaggcggat gggcgtgtcg ggcgccggcc ccagaagtcc cgcgtagaaa ctgcgcgccg
     7321 ccccggccag cgcgataagt gactcggccc gcttgcgctc gtcggccgac gcgccgggcg
     7381 ggacgagcgc gctcacgccg cgcgcctccg cactgccctc gattgtctcc cacttccccg
     7441 tcaggaagaa cggctgtgcg ctcaaggcga cttcgtgagt cgcgcccgtg gcgcggccgg
     7501 cggagactac gcccgcgcct tcgccgctcg cgccctcgac cgtcagacgg aagggtgcgt
     7561 agtcggctcc gcgcggggcg atgggcgtgt tgggcgtcgg ataccagtgg gagagcggga
     7621 gcaactgcgc gccctcggcc gagagggtgg cgagtccgtt gttctcggag accgtcaggc
     7681 ggtagtcgat ctggacgctc gtcgcgccgc ccggcggcac ggcctcgggg agcgcgaccc
     7741 tgaccatcag gagctttgtc tgcccgtccg tctcggtcgt gaacttcgcc ggcgcgccgc
     7801 cgactgttgc cgcgctgacg atggcggccg gattgacgcg cgccgtcagc gtgcggcccg
     7861 ccgcgccgcc gacgttggtc gcgttgatcg ttgcgcgcac ggcgagcgcg cgctccgtgg
     7921 cggacaagcc gccgacgttg gccgtgatgt cgtaacggat gacctgccac gccgagcggg
     7981 cgtccgcctg cgcccacacg tcctgagcca ggaggagcgg aataaataga aggagaagaa
     8041 tgcgaagttt tttcatgtac cgtttgatga atctatgttg tgtgtgacgc aagttaactc
     8101 accgcggagt cgcggaggac gcggaggatg cgcggaggct tgaattaact ctgcggcgtg
     8161 aactctgcgc cctctgcgcc tccgcggtga gtcaattgtc atttatcact caactcccga
     8221 gccaaaaatc accttcacga ttcgacagcc gcctcacgcc ggacgagttc gtgcaggtcg
     8281 cgcagagtct tcaggagcgc cgccagacgc gcgagcggca gcgcgttcgg cccgtcggag
     8341 agggcgcgct cgggcgcgtc gtgtacctcc atgaagaccg catcgacccc gcaggccacg
     8401 ccggcgcggg cgagtggttc gatgtactcc gactgcccgc ccgttgcacg cccgaggccg
     8461 cccggcagtt gcaggctgtg tgtcacgtcg aagacgacgg gcacgccgaa agagcgcatg
     8521 acggggaacg tccgcatgtc aacaaccaga ttattgtaac cgaacgaggc cccgcgctcg
     8581 gtgagcagca gacgctcgca acccgcggcg cgcgccttct ccacgatgtt gcgcgcgtcc
     8641 cacggcgaaa gaaactgccc cttcttgacg ttgacggcgc ggcccgtctc cgcggccgcg
     8701 acgatcaaat cggtctgccg gcacaggaag gccggtatct ggagcacgtc ggcgacgccg
     8761 gccgctagct gggcctgcgc cacgtcgtgt acgtccgtca gcacgggcag ccccgtctct
     8821 tccttgacgg cgcgcagggc gtccaaggct tcttcgacgc cgatgccgcg aaaggactcg
     8881 tgcgatgaac ggttggcctt gtcgaacgac gccttgaaga cgtagtcgag acctgcgtcg
     8941 agcgcgcgct tcgcgcactc gcgcgccatc atcagaacgt gctcgcgcga ctcgacgacg
     9001 cacggccccg cgatcaacgt caggcggccg tcgccgaacg tggcgtcgcc aaccctgaac
     9061 ggtttgatgt tcatctcctc cacagttcca ctcaacgcgg ctgccctccc tcattctcca
     9121 acttgcggcg caactcccgc aggacgcgtt cgccgcgccg ccggttctcc gcgttcggct
     9181 cctgctggat gtagatttcc aactccttcg cggcctcggc cgtgcgcccg gtctcggcgt
     9241 acatcatgcc gagacggaag tgggcctcgg cgaagtcgcc gccgcgctcg tcgatggccg
     9301 cgcggaaggc cgactccgcg cccgtgtagt cttcgagcat ggcgagggcg aagccgcgct
     9361 gattgtgcgc gtcggcgtcg cgcccgttgt tctgctcggc ggcgcgggct aacgcgtcgg
     9421 ccgcctcgcg ataacgtcgg cggttgaggt gcgccagtcc gatctgaaag aaggcgtcgg
     9481 ggtaggtgcc gccgcgactt ttgacggcgc cgtcgtatgc ctcaatcgcc tcgtccagat
     9541 cgccgcgcgc ctgaagggcg aagccgagat tgtactgcgc cgccgggtag ttgccctgct
     9601 tctgctcgac ggccctgcga aactcctcgg tcgcctcgcc gagcttcccc tgcatcatca
     9661 gcacgcgccc cagacggttg tgcgcctcgg gaaagtcggg ctgaagctca agggcgcggc
     9721 ggtagtcgcg caccgcgccg tcgaaattgc gcgaagagaa atagtatgcg ccgcgctcat
     9781 aaaactcggc ggcagagcgt ggctcggaga c
//
LOCUS       DASVES010000222         7353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1234180, whole genome shotgun sequence.
ACCESSION   DASVES010000222 DASVES010000000
VERSION     DASVES010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234180"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1671
                     /locus_tag="VF240_08920"
     CDS             262..1671
                     /locus_tag="VF240_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175366.1"
                     /translation="MRTRRLLLPSLFLFALLSCLAPNALANGGLNVSARDAVSEDAAL
                     RASALKTLREAGPAGLEALFAEFADEIALRAGGQPAAGGAEWARIAEALDAVSQQRDG
                     FASRLYWYTDFEEAKHAARASGKPILSLRLLGKLSEEYSCANSRFFRTVLYANTSVSK
                     FLRDNFVLHWQSVRPAPRVTIDFGDGRRLERTVTGNSIHYVLDPEGWPIDALPGLYGP
                     GAFVRELERAARATRASARLGGEARNQFLFQYYNEQAVRLMDSVRSDAQAAVVRLPAE
                     VIQRQIGGVPARGTITPPPPTKSTAIQAMPRAMTKMMIEDRLVREVTNNITYRPGVMQ
                     RAINEDGWAKIAGLHLPDARMDAASIALLAAHNPHTRPAVRAMTAADTTGAGGRFARA
                     VANLERTMALDTVRNQYLMRPVLLRWLISGAGLAGVEEFNRRVYAELFLTPDSDPWLG
                     LAAPDSYTALLDDGLVTNR"
     gene            2179..3024
                     /locus_tag="VF240_08925"
     CDS             2179..3024
                     /locus_tag="VF240_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7175367.1"
                     /translation="MNLTKRIGGHDGLSAARHLLLMTALAAASLFGRAPDVLAQQQVQ
                     PDENLKELREDVRSLKEGMTAVQRELQEIKKLLSKQPVVAAAPANEAPREILLDLGGA
                     PSKGDAGARLVLVEYSDYQCPFCSRYVRDTLPLIERDYIKTGKIKYVFRDFPLESIHP
                     NALVASEAAACAGEQGKYWEMHDRLFGNQGALAVADMAAHARAVGVNQPNFQQCLAGG
                     RHKEPIRKDMAEAISLGVSGTPTFFIGLASPDSSKVKVLRVMKGAGSYVNFKEVLDGA
                     LAALP"
     gene            3627..7346
                     /locus_tag="VF240_08930"
     CDS             3627..7346
                     /locus_tag="VF240_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX7175368.1"
                     /translation="MDELINLSPEQVGKRPITAKIKITTEERRSHEEALRRLEEIRSG
                     IGAPTSAFLTIGGWPAIQYGRIDERPSPGQFPRPQFKDKKMYRITTAIAVGDLLVQLE
                     AILPSDANPGLIHQAEAISRSAFFSAKADPKKTQDEVGRLRKNARPRSAPLALTTGDQ
                     VAAFTPLPASLRPTTSGAGGGAAKVLPLSKQDPDNSAEPGLTRRVISNRNGELEIAVS
                     PNGRNIVIGQQSAFRSSTDGGQTFPFNGTIPYTGGDPSLAWGPSNAFYYAGIAGGGGC
                     QAADAAGPQGYTCTGMARSTDNGQTFPFVTRAVACPNDTNPASIPGACFPDQEHIAAD
                     RVSTGAGGGDRVYSTWRNFDATDQDPGLVCSQDSGQTWSAPIVVGSGSFPRINVGQDG
                     FVYVVYRSGGDYMLNKYNPCPAAGAPTVVPGFPVVVMAQNEITCPFPGHDRCDQDPSS
                     QMLAVDDTNPNHVYFSVAINGGSATNDNIVVRDSLDGGLTWPAGRVVQANDSIPGKRI
                     MPWICTTGGEAFVTWYDRRSTPANQNDLTEYYAGRVRLDGASNLVVGSEIKLSEVGDP
                     WCASGWPCGTRGAPGASEQCSVQPQLAGFCCSSLDANGNCVGSGNRCDFSDGCTIGGE
                     TCQGVGGGCPKYGDYNGNACAAGRIFAAYASATAPPSITPASTGIDIFFTSKLVGDVP
                     QLQVPGGVNFGDICVGGTGTQTLNVCNTGKADLEINDITSNNPRFAVTTTTQGYPVVI
                     SHDFCFPFQVTFNPTATGAQTATLTITSNDPALPTATVQATGSGTRQAIDTLIPNSGN
                     FGQVCVGSFKDLNLTINNSGGCDLVVNSVTSTGADASQFIVAGDNTYPITVAPGVSVQ
                     VPIRFQPTSIGNKATTIRVNSNDPNAPNKDVAVSGTSDPGDIRVTGSTNFGDICGGSL
                     AEKTVNICNVATACNLNVTNVRLAANPDGTGSCSDFTLVNNPFPTPVSHDSCLDLVIR
                     FTPTSAGPKQCTLVVESDDPQTPSVKTLVTATTPLASIDVSPDQSFLPEVNQSIGFCT
                     TLQPFPISNKGTCPVKVTDVSVNDPNNFGLSALPSFPINLQPGHTVGDGALKTVFKPI
                     APLDRERDGVLSVTYESDPIAHVLTTETRDLCGEVVNTGARVLVRVGLVPFNGEVESI
                     RLNRINANRNKNNVDTVDNARNLPLQTFAYPAGSACTPFQYHREYGTVGNPIQLLPGS
                     YTVTATAVINGKRKTKTVAFDVATCDFNPTIFVDF"
BASE COUNT         1385 a         2606 c         2179 g         1183 t
ORIGIN      
        1 caacgcgcgg gcgccggcgg cgcatcccac ttccgaccgc ctcgccacaa tgattcgtgt
       61 gaagacgccc tcccggcgtc tctcgcgcgg acgcgtcact tcccatcggc tttgcccgca
      121 aagtccccga gaagtcgctt cacccgaaaa gtgtgacgca gttaaaggat ttagcccaga
      181 cttttcgggt gaaatgtgtc aatcgggact ttgcggggac tttgcgggca aaacccttcc
      241 catctcaaga gaggttttct tatgcgaacc cgccgcctgc tattgccgtc cctttttctc
      301 ttcgcgctgc tgtcctgcct cgcgccgaac gcgctggcga acggggggct gaacgtctcg
      361 gcgcgcgacg ccgtctcgga ggacgcggcc ctgcgcgcct cggccctcaa gacgttgcgc
      421 gaggccggcc ccgcagggct tgaagcactg tttgcggagt tcgctgacga gattgcgctc
      481 cgtgcgggcg gacaacccgc agcgggcggc gcggagtggg cgagaatcgc cgaggcgtta
      541 gacgccgtga gccagcagcg cgacggattt gcgtcgcgtc tctactggta cacggacttc
      601 gaggaggcga agcacgcggc ccgcgcgagc ggcaaaccca tcctgtcgct gcgcctgctc
      661 ggcaagctga gcgaggagta ctcctgcgcc aacagccgct tcttccgcac ggtgctctat
      721 gccaacacgt ccgtctcgaa gttcctgcgc gacaacttcg tcctccactg gcagagcgtc
      781 cggcccgcgc cgcgcgtgac catcgacttc ggcgacggcc gccgccttga gcgcaccgtc
      841 accggcaaca gcatccacta cgtcctcgac cccgagggct ggcccatcga cgcgctgccc
      901 ggcctctacg gccccggagc cttcgtgcgc gaactcgaac gcgccgcccg tgccacacgc
      961 gcctcggccc gcctcggcgg cgaggcgcgc aaccagttcc tcttccagta ctacaacgag
     1021 caggccgtgc gcctcatgga tagtgtgcgc tcggacgcac aggcggccgt tgtccgtttg
     1081 cccgccgagg tgatccagcg ccagatcggc ggcgtgccgg cgagggggac catcacgccg
     1141 ccgccgccga ccaaatccac cgcgatccag gccatgccgc gcgccatgac gaagatgatg
     1201 atcgaggatc gactcgtgag agaagtcacc aacaacatca cctaccgccc cggcgtgatg
     1261 cagcgggcga tcaacgaaga cggctgggcg aagatcgccg gcctgcacct gcccgacgcg
     1321 cgcatggacg ccgcgagcat cgcgctcctc gccgcacaca acccccacac gcgccccgcc
     1381 gtgcgggcca tgaccgccgc cgatacgacg ggcgcaggag gtcgcttcgc ccgcgccgtc
     1441 gccaacctcg aacgcacgat ggcgctcgac acggtgcgta accagtacct gatgcggccc
     1501 gttctgctcc gttggttaat cagcggggcg ggcctcgccg gcgtcgaaga atttaaccgg
     1561 agagtctacg ccgaactctt cctgacgccc gactccgacc cctggctcgg cctcgccgcg
     1621 cccgacagct acaccgccct gctcgacgac ggcctcgtga caaacaggta gcatgtcgct
     1681 gggagcgcac gcgccgatgc gtgtgctccc cgacatgacg tcgccgctca gccgtttcgg
     1741 agaacgtgtt agcaaaaatt cagttgacat cctcacgcga tgaaggtatg ttgcgccggt
     1801 caaatctgct gcctgaagtt tctttgccca ggttagaatt gccccgcaga ttctcctttt
     1861 gaatatgatc cgcttccggt ctcccatctc gacatctcgt cgaacagctt tccagggtcg
     1921 gccctgtgtc tgcgtgaccg gccattagcc gactacttgc cacttttcaa gaatcgccac
     1981 ggtcgtgtag tggttcagtt tgaagctgaa aactatattt tcatccgcgc gcgtgcggcc
     2041 ccatgaaatg gcctgccgcg acatcgcata aatttgtagg caatagtctt tagggtgatt
     2101 gcctggggca atccgattgc ggagcggtct tgccgcaatt ggaattgacc cattaaccac
     2161 tgtcggctgg acattgacat gaatctcacc aaacgaatcg gcggccacga cgggttgtct
     2221 gccgcccgcc accttttgct gatgacggcg ctggccgccg cgtcactttt cggccgcgcg
     2281 ccggatgtcc tggcccaaca gcaggtgcaa cccgacgaga atttaaagga attgcgggaa
     2341 gacgttcgct cgttgaagga ggggatgacg gccgtccaaa gggaacttca ggagattaag
     2401 aagctgctct cgaagcagcc cgtcgttgcc gccgccccgg cgaacgaggc tccgcgcgag
     2461 attcttctgg atttaggcgg cgcgccctcg aagggggacg ccggcgcgcg gctcgtgctc
     2521 gtcgagtatt ccgactacca atgccccttc tgctcgcgtt acgtccgcga cacgctgccg
     2581 ctgatcgaac gcgactacat caagacgggg aagattaaat acgtcttccg cgacttccct
     2641 ctggaatcga ttcaccccaa cgcgctcgtg gcctcggagg ccgccgcctg cgccggcgag
     2701 caggggaaat actgggagat gcacgaccgt ctcttcggca atcagggcgc gctggccgtc
     2761 gccgacatgg ccgcccacgc acgggccgtc ggcgtcaacc agccgaactt ccagcaatgt
     2821 cttgccggcg gccggcacaa agagccgatc cgcaaagata tggccgaggc catcagcctc
     2881 ggcgtgagcg gcaccccgac cttcttcatc ggcctcgcat cgccggacag ctcgaaagtt
     2941 aaagtcctgc gggtgatgaa aggggccggt tcctacgtca acttcaagga agttttggac
     3001 ggcgctctcg ccgctctacc ataagccacg ggcggttcgc cgcccctatt aggagggccg
     3061 aaggcatccc acgcgaagct cgggtttcat ccgtagaata actttcaggc acgccccact
     3121 cgtcggaatc gctaccccac acaacacaaa cgaaccccac aaacctcgcc gcgtttaact
     3181 cctcgctaaa cacaagtagg agacacgcaa tgcaacgtcg tagcctttcc ctggctgtgg
     3241 gcgtcatcgc actgctggtc agcttttcgc tcccccggac ttatgcgcaa gtcgtgccgc
     3301 aagattcggg ggccgcgcag gccgtcaact ccgcccagcc agacgcggcc gacccggtaa
     3361 aacgggtcgg tcggacggag tcgccgacgc cgcctgccgt gagcgttgcc aagctgtcgc
     3421 cagccgccgc cctcgccgcc caaccacctc cgaactctaa ctcttccgcg ggaaaagact
     3481 cggggaagcc aaacgccaca gcaccgcaaa ataataacgg gcaagaagaa cgcaagcccc
     3541 tcaccaagag actcgagaac acccgcaagg gctacgccgt ccaatatcca gaggattggt
     3601 cggtcggccc gaaccggttc gccaacatgg acgagctcat caacctctcc cctgaacagg
     3661 tggggaaaag gccgattacg gccaagatca aaatcaccac cgaagagaga agaagtcacg
     3721 aggaggcgct ccgccgtctg gaagagatca ggagcggcat cggcgccccg acctcggcct
     3781 tcctgaccat cggcggctgg cccgccatcc agtacgggcg catcgacgag aggccgtcgc
     3841 ccggtcaatt cccccgcccg cagttcaagg ataagaagat gtacaggatc acgactgcca
     3901 tcgcggtcgg agacctgctc gtccaactgg aggcgattct cccgtcggac gccaacccgg
     3961 ggctgatcca ccaggcggag gccatcagcc gcagcgcatt cttctcggcg aaggccgacc
     4021 cgaaaaagac gcaggacgag gtggggaggc tccgcaagaa cgcgcgccct aggtcggccc
     4081 cgctcgccct cactacgggc gaccaggtgg cggctttcac cccgctcccg gcgagcctcc
     4141 gcccaacgac ttcgggtgcc ggaggcggcg cggcgaaagt gctgcccctc agcaagcagg
     4201 accccgataa ttcagccgag ccggggctga cccggcgcgt cattagcaac cgcaacggcg
     4261 aactcgaaat cgccgtctcg cccaacgggc ggaacatcgt catcggccag cagagcgcct
     4321 tccgaagctc gaccgacggc ggccagacgt tcccgttcaa cggcaccatc ccctacaccg
     4381 gcggcgaccc ctcgctcgcc tgggggccga gcaacgcctt ctactacgcc ggaatcgccg
     4441 gcggcggcgg ttgccaggcg gccgacgccg ccggcccgca aggctacacc tgcacgggca
     4501 tggcgcgctc gaccgacaac gggcagacgt tcccgttcgt cacgcgggcc gtcgcctgcc
     4561 ccaacgacac caacccggcg tccatccccg gcgcctgctt ccccgatcag gagcacatcg
     4621 cggcagaccg cgtcagcacc ggcgccggcg gcggcgaccg ggtctactcg acgtggagga
     4681 atttcgacgc caccgaccag gaccccggcc tcgtctgctc gcaggacagc ggccagacct
     4741 ggtccgcccc aatcgtcgtt ggctctggct cattccccag aatcaatgtc ggtcaggacg
     4801 gttttgtcta cgtcgtctac cgttccggcg gcgactacat gctcaacaag tacaacccgt
     4861 gcccggcggc gggcgcgccc accgtcgtgc ccggcttccc cgtagtcgtc atggcgcaga
     4921 atgagattac gtgtccgttc cccggccacg accgctgcga ccaggaccct tcgagccaga
     4981 tgctcgccgt tgacgacacg aacccgaacc acgtctactt ctccgtcgcc atcaacggcg
     5041 ggagcgcgac gaacgacaac atcgtcgtcc gcgactcgct cgacggcggc ctgacgtggc
     5101 ccgccgggcg ggtcgtccag gcgaacgaca gcattcccgg caagcgcatc atgccgtgga
     5161 tctgcaccac gggcggcgag gcgttcgtca cctggtacga ccgccgctcc actcccgcga
     5221 accagaacga cctgactgaa tactacgccg gccgcgtgcg gctcgacggg gcctccaacc
     5281 tcgtcgtcgg cagcgagatc aagttgagcg aggtgggcga cccctggtgc gcttcgggct
     5341 ggccgtgcgg aacgcgcggc gctccgggcg cttccgagca gtgctccgtc cagccgcaac
     5401 tggcgggctt ctgctgctct tcactcgatg ccaacggcaa ctgcgtcggc tccggcaacc
     5461 gctgcgactt ctccgacggc tgcaccatcg gaggcgagac ctgtcagggc gtcgggggcg
     5521 ggtgcccgaa gtatggcgac tacaacggca acgcctgcgc cgcggggcgc atcttcgcgg
     5581 cctacgcctc ggccaccgcg ccgccttcca tcacgcccgc ctcgaccggc atcgacatct
     5641 tcttcacgtc gaagctcgtc ggcgacgtgc cgcagctaca ggtcccgggc ggcgtcaact
     5701 tcggcgacat ctgcgtcggc gggacgggaa cgcagacgct caacgtctgc aacaccggca
     5761 aggccgatct cgaaatcaac gacatcacct cgaacaatcc caggttcgcc gtgaccacga
     5821 cgacgcaggg gtatccggtc gtcatcagtc acgacttctg cttcccgttc caggtgacgt
     5881 tcaacccgac ggccacggga gcgcagacgg ccactctgac catcacgagc aacgacccgg
     5941 cgctacccac ggccaccgtg caggcgaccg gttcggggac gcggcaggcc atcgacacgc
     6001 tgatcccgaa cagcggcaac ttcggtcagg tctgcgtcgg ctcgttcaag gacctgaacc
     6061 tgacgatcaa caacagcggc gggtgcgacc tcgtcgtcaa cagcgtcacg tccacggggg
     6121 ccgacgcctc gcagttcatc gtcgccggcg acaacaccta cccgatcacg gtcgcgccgg
     6181 gcgtttcggt gcaagtgccg atccgcttcc agccgacgag catcggaaac aaggcgacca
     6241 ccatccgggt caacagcaac gacccgaacg cgccgaacaa ggatgtggcc gtctcgggca
     6301 cgtccgatcc gggcgacatt cgcgtcaccg gctcgaccaa cttcggcgac atctgcggcg
     6361 gctctctggc ggagaagacc gtcaacatct gcaacgtcgc gaccgcctgc aacctcaacg
     6421 tcaccaacgt gaggctcgcg gctaaccctg acggcacggg gtcgtgcagc gacttcacgc
     6481 tcgtcaataa cccgttcccg acgccggtca gccacgactc gtgcctcgac ctcgtcatcc
     6541 gcttcacgcc cacctcggcg gggccgaagc aatgcacgct ggtcgtcgag agcgacgatc
     6601 cgcagacccc ttcggtcaag acgctcgtaa cggccaccac gccgctcgct tcgattgacg
     6661 tgtcgcccga ccagagcttc ctgccggagg tcaaccagag catcggcttc tgcacgacct
     6721 tgcagccgtt ccccatctcc aacaagggaa cctgcccggt caaggtcacg gatgtctccg
     6781 tcaacgaccc gaacaacttc gggctctccg cgctgccgtc cttccccatc aacctccagc
     6841 ccggccacac ggtgggcgac ggcgcgctca agacggtctt caagccaatc gcgccgcttg
     6901 atcgtgagcg ggacggagtg ttgtcggtca cttacgagtc tgatcccatc gcgcacgtcc
     6961 tcacgaccga gacgcgcgac ctgtgcggcg aggtggtcaa cacgggcgcg cgcgtgctcg
     7021 tgagggtggg cctcgtcccg ttcaatggtg aggtggagag catcaggctc aaccgcatca
     7081 acgccaaccg caacaagaac aacgtggaca ccgtggacaa cgcgcgcaac ctgccgctcc
     7141 agacgttcgc gtacccggcg gggtcggcct gcaccccgtt ccagtaccac cgcgagtacg
     7201 gcaccgtggg caacccgatc caactgcttc ccggctccta cacggtgacg gcgacggcgg
     7261 tcatcaacgg taagaggaag acgaagacgg tcgcgttcga cgtcgcaacc tgcgacttca
     7321 acccgacgat ttttgtcgac ttctaacggc taa
//
LOCUS       DASVES010000223        35612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_195252, whole genome shotgun sequence.
ACCESSION   DASVES010000223 DASVES010000000
VERSION     DASVES010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 35612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35612
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195252"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VF240_08935"
     CDS             complement(<1..594)
                     /locus_tag="VF240_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175369.1"
                     /translation="MAEERNLATARAPEDVTADPDATKEELQRRMDEARESITQTVTE
                     IKESVVSQYQQVRENISDTLDWREQYRRHTLPVTLGAAGAGLLLGYTIGGALFGRGDD
                     DYDEDEYEQDDAQLLLPAATHAYASQPILGGSPSARSARRSSMSTAAAAAPAFAAADV
                     GPDTRPGYASADAPQELASPQPSEPEGPSLFSRFRETK"
     gene            complement(573..1031)
                     /locus_tag="VF240_08940"
     CDS             complement(573..1031)
                     /locus_tag="VF240_08940"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7175370.1"
                     /translation="MSQLERGAAGAAPARREPTQAEIDSLPSLFGRLGDDVMTLVDAK
                     LGLIKVELKEDASVYARNGVLMAVGGLVAAIGFMLFNVAIAFFVSKLFSFADPRLNYA
                     LGFVITGLVYLVAGAVILLMAKGRLAAHDPTPNRSLDEIRKDKQWLKNEI"
     gene            complement(1136..1684)
                     /locus_tag="VF240_08945"
     CDS             complement(1136..1684)
                     /locus_tag="VF240_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HEX7175371.1"
                     /translation="MSANKPNNDSPNSTTGSKGTTGTTGGSSATGSSSHTGTGYTGGT
                     GATDAGNKPSSGSASGSTSGSASGSTSGSGPASSSGGGSLARSSSTTSTGGGAGAATG
                     LASQAQEYGQKIADAASTAKDYVTEKAGVVGEKFQDLRNKDYGQMADEAKQYARQNPG
                     QALLISAAAGFLLGILLRGSRR"
     gene            2067..2870
                     /locus_tag="VF240_08950"
     CDS             2067..2870
                     /locus_tag="VF240_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7175372.1"
                     /translation="MTRQPDKAGATPAAQIPAPAAAGRMAGGDRRRQIVDVAMRLFSE
                     RGFRGTTTKEIATAAGVSEAIIFRHFATKEDLYTAIIDHKACSGEMADMEQPVAEAIR
                     CSVAEHMSAKDDRAVFAHIALHMMQHHERDTEFLRLLFYSALEGHQLSRMFWERNVRT
                     LYEFLGSYIRERQRDGAFRGADPLVVVRAFAGMIIHHSVTNTLWDKSRSLLNIPNEEA
                     AREFTEILLRGIDAGPAKAAPARAGRADVAAIARASATSRRAPRAKKGK"
     gene            2906..4171
                     /locus_tag="VF240_08955"
     CDS             2906..4171
                     /locus_tag="VF240_08955"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7175373.1"
                     /translation="MFCLRQLQTFTLAVAVAGSGLAAAACGGSRAETNKKEGGAATQA
                     TPAAVDVTTAPAVTRQLPRFIEATGSLAADEQTDVAPIVGGRVSQVYADLGSYVQRGQ
                     VLVRLDGEDARLRLQQLEAQLEQARSSVRQAEERIGLRAGARFDPTRVAEVGAARAAL
                     DLAEKQLRRYERLIESGDVSRSAYDQQKAQRDQLRQQYEAALAQAHQSYAGVQTARAA
                     AMAVASQVEQARKAVRDVVIVSPISGFVSERTADVGEFVSTSMKIATIVRTNPLRVRI
                     DIPEQAISNVQTGQSITLLTSAYPDRAFAGRVARISPNISAASRTMTVEAEVENSAGL
                     LKPGQFVTARILLPESDPAVLVPVRAIRTDGTTSRVFVIRDGVAQERLVQTGQQEGDL
                     VEVRGNVAADEPVATSNVELLSDGMGVRQ"
     gene            complement(4201..4506)
                     /locus_tag="VF240_08960"
     CDS             complement(4201..4506)
                     /locus_tag="VF240_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175374.1"
                     /translation="MSNEKVTKPTIETVLERLVSFEERMDTKFNSLEERFDLNLGTLE
                     ERLNARIDGVDAKIDRLDIRFDRLETVVNATRGDILELRADIREVRTQFREHFPATP"
     gene            4631..7909
                     /locus_tag="VF240_08965"
     CDS             4631..7909
                     /locus_tag="VF240_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7175375.1"
                     /translation="MQKLAEICVRRPVFATMIILSLVVVGVFSFFSLGVDLFPKIDFP
                     TITVTVVNPGAGPQEIETEITDKVESAVNTISGIDELRSTSVEGVSQVFVTFVLDKDA
                     DVAAQEIRDKVNLIVPDLPETAEEPTIQKLDTDAAPVLRLVVSAPRSLRDVTDVADKQ
                     IKKRIESIGGVGQVQIIGGARREVQVKVDPDKMRAFNVTPADVVAAVRQQNLEVPGGR
                     VDEGARELTVRTMGRITNPADFNNISVGSRGGYPVKISDIGYVEDSSEEQRTAAFLNG
                     QPAVTLVVSKQSGQNTVAVADAIKERLADIQPTLPKDVKVEVIGDSSIFIKAAVESIQ
                     THLIEGGILAALVVFIFLWNFRSTIIAGIAIPTSIVATFGLMGAMGYTLNQITMLALT
                     LMVGIVIDDAIVVLENIYRYIEEKGYEPFQAAIEGTKEIGLAVMATTLSLLAVFLPVG
                     FMKGIVGRFMSSFGFTASFAIAVSLLVSFTLTPMLSARLIKRRREEHREAEAVTNDDQ
                     VQSVSLSEDSGPTPEVPAMPAEVPATDEARSAKTAKDSRFYRPIDRTYTWLLHWAMAH
                     RWAVVTLCVLVVLSTAPLFMLIGKNFLPVDDQSQFEVTLRAPEGSTLASTKTVTERIA
                     KELRELPGVTDTLTTIGGGQQQLVNSANIYVKLVDIGDRKQSQIELQDEARKILEKYP
                     KELRPAVGQVQAFSGGGFRNADVQYVVSGPDLEKLTDYSNRFLEKLQAIPDVVDPDTS
                     LITGKPEVRVHIDRARAADMGVRVGDIAQALNVLVAGQVATTYNQGTDQYDVRVRATG
                     ESRASQKALENLFVPSAKTGWTNLSSLVRTEEGTGPSAIERLNRQRQVIISANVKPGG
                     SQAAVTAALDEFAREINLEAGYTTGTFGRSKELQKSGYYFMLAFALSFIFMYMVLAAQ
                     FESFIHPITILLTLPLAVPFGILSLLVMGQTVNIFSGLGLLLLFGVVKKNAILQIDHT
                     NALRSHGMERLDAIIQANRDRLRPILMTTIALVAGMIPLVISTGPGAGTNRSIGVLVV
                     GGQSLCLLLTLLAVPVFYSLFDDLSHSRVPARIGRRVGGVFAWARRRAATATSSFFGI
                     VGKN"
     gene            7982..10402
                     /locus_tag="VF240_08970"
     CDS             7982..10402
                     /locus_tag="VF240_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7175376.1"
                     /translation="MSKRFRKLTCRLTALALLVCSSAPVGVLAQQTAGARARQQQQQQ
                     RQQGERREEPQKFTGTLFGGDASAETREAEPREGREVEAAGVATPAARVVKKDVTPQS
                     YVRVTLKPAPRYEEAALHTATAQQPQQGQQTASPAAPSANNPPPTQGAPAQGPQTASP
                     TQSQTQQSANPAAPAQGQQQPAQPAVPPASPTQGVSTQTQGEGTQPAQGQPGQPQQPS
                     PNAPGSQVGAPQGPRAPGRESVPMAAPADEGLQVPQVATDFRSAEGPFPALERVGVQM
                     GDQRALSLREAIELALANNKDIEVARTNVRAAEFDLQAARGVYDPRFLTNSYYERSET
                     PAASFLSGSTSGAVTQSGFFSNTSFQGLVPKYGGGYRVDFVSNRVTTDNQFAALNPQY
                     PTALTFSYTQPILRGRAFDQNRRFIEIASRNLTLTDAQFRQRAVETITQVQRAYWDLV
                     FALRNLQIQRDAVRDARAQLEHNRRLVVEGLLAPIDVVAAEAQVSGFEQSVYTALDEV
                     GRSENVLKNLIAENREAALWNVALVPADPVELRPPTVDLQAAMDSALKSRPEIQTSDV
                     AASINEVEQRFAREQAKPQIDFVGSYGIVGLAGTLNTSDAANPFTASSTQLRERINQL
                     AAAVNGLTGRNDIVPIPPPAPQVLPDVLVGGYGQSIANLAANRYNNFRVGVTVNLPIG
                     NRTARAQLGRALVEGERIGTQREQLEQLIQVDVRNSLQTVRTAESRLRAAASARSASE
                     QQYASEKRKFDAGQSTLFLVLERQTSLTTARGNELRAQTELNKAIAELQRATGNALEA
                     NRVEVRVR"
     gene            10438..10800
                     /locus_tag="VF240_08975"
     CDS             10438..10800
                     /locus_tag="VF240_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175377.1"
                     /translation="MEAQRAEILNRLSAHGWSVRCVDDYELEWWADEMWLLESTWSPA
                     GGRAWVTFLVDPQTSDVANRRKGKGVWALLASPEKPTRWQASEGEFSLGLGRGWEDEL
                     PALLEYLDAVRAGSRQDA"
     gene            10793..11296
                     /locus_tag="VF240_08980"
     CDS             10793..11296
                     /locus_tag="VF240_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX7175378.1"
                     /translation="MPESFSTKLLRWKFNLFPAYRGTGGRITYIAADLREVRVRLPLN
                     WRTRNYVGTIFGGSLYAAVDPIYMLMLIWALGPGYVVWDKAASVRFKLPGRSTLHAHF
                     RLDEDELRAIQSELENARSINRVYSVDLKDAEGKVHATVEKTIYIRRRDAGSKSKDTG
                     GRAGECY"
     gene            complement(11290..12288)
                     /locus_tag="VF240_08985"
     CDS             complement(11290..12288)
                     /locus_tag="VF240_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7175379.1"
                     /translation="MTTSAGARVSLPSAVRRRCVALGLLLSVLVIAVFLLPSAHGTPP
                     SPSESDERAADASLLEVGSARKLVAPPAEPAEIKIVSYNIRYRDGEDLQELIRLLKDD
                     PEIGGAALIGLQEVDRNKERTGRANTPRLLAEALGMHYAWAAPPPPPKDGAKPPKKEP
                     EEETGVAILSPYPLSDVERLVLPHEGPNRRRRAGIGATVRIGRDDVRFYSVHAETRMP
                     VEKKVDHWRIVLDDLRRHPKIQHVVVTGDFNTIKDKDVRAARKLFTEAGFATPFRDED
                     ATFKVVFFDFKLDWVWLRGLTSAGHGIDKKVGLSDHWPLWVRARLEKTAKTTPSTQ"
     gene            complement(12384..13328)
                     /locus_tag="VF240_08990"
     CDS             complement(12384..13328)
                     /locus_tag="VF240_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7175380.1"
                     /translation="MKSRLIVNPVSGTDEAPEQLRVINERLRAGVGEMDIVMTVAQGD
                     AARAASEAVGAGYEQLFVAGGDGTLNEALNGVAGVPGALESVVFGLIPLGTGNDFARA
                     LGIPESVEEAVDVLLEGREVRVDVGALGERRFVNVSAGGFIAEVSDAVDPALKSLAGK
                     LAYLIGGAQVLFDYEPPHARVRARGIGDDGAGAFEREMQVQMFAACNSRMVGGGRPIA
                     PDALIDDGLLDLCIVEAMPTLEFIGLLTSIASGEHVEDERVIYLRAREVEIDFDRAVK
                     VNTDGEVLETDRCRYRVEARAARFLAGDAPYTTQGESS"
     gene            complement(13563..14333)
                     /locus_tag="VF240_08995"
     CDS             complement(13563..14333)
                     /locus_tag="VF240_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7175381.1"
                     /translation="MKLTVLGSGTAVPHPRRSSSAHWLETRGGTLLLDPSATAFHRAA
                     EEGLDWPGLDAVWVSHFHLDHVGGLAPFLFGTKHAPQTRARRKPLHIFGPHGARKLLE
                     TFDGAGNYKLFEQPFPVEVREVAPRAEFEILPGVGAATFSTPHSSESLALRLEETAAD
                     GASLAYTSDTGYADAVADFARGVKLFLLECSFFRAKPLETHLILEEAIRLARRAAPGR
                     LVLSHLYPEWDDLDLAAEAAKFWDGETIEARDGLRLEV"
     gene            14503..15060
                     /locus_tag="VF240_09000"
     CDS             14503..15060
                     /locus_tag="VF240_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX7175382.1"
                     /translation="MHGVVSLLDDEHYRLVEDVWAELSRKFGVHGIYSTPFPHFTYQV
                     SEEYDTKTVESALRAVAEASTPFRVRTAGLGVFCAARPVLHVPVVRSPELERFHDEVW
                     RVLGRPPAGEVARFYDSQMWVPHVTLAQGDIDSERLGEIVRVLAARSFHWEMEVNNLS
                     LIYDTGTEQGLRCRFNFGNGRGAVA"
     gene            15150..15701
                     /locus_tag="VF240_09005"
     CDS             15150..15701
                     /locus_tag="VF240_09005"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7175383.1"
                     /translation="MSNEDDREGAAALPVQVYIFNQTYNLRSGSDEEYVRRVAQRVDE
                     RMRQISSMAPYHDPMRVAVLAALHMADELEQSRETAQEPEDARPEEEETAASAAATAG
                     SAPDARAGLEGASSWFESVFDSEFTGNRSSGERLSDRVSERLQTRRQERRRPIVIDAD
                     DTDGTPATDRRADAGPRDNQSRD"
     gene            15732..16478
                     /gene="bshB1"
                     /locus_tag="VF240_09010"
     CDS             15732..16478
                     /gene="bshB1"
                     /locus_tag="VF240_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX7175384.1"
                     /translation="MTDQPVELDVLAVYSHPDDAELAAGGTLLKLKHLGHRTGVLDMT
                     RGEMGTRGTREIREGEAREAGRLLQLDARLNLDQPDGHVWPTEDARRALVRVLRACRP
                     KVILTAHWDDPHPDHANTCRIAREAARLASMRRYDEEHGQAAIKMPALAHSVYSRLVV
                     ASFIVDVSDFVEDKMAAVRAHASQFWSEGSKEPSTRISDEGFLNQIEFRLRYYGSLIG
                     VAAGEGFYVREALNVEDPVALLTRPMNIYS"
     gene            complement(16567..17550)
                     /locus_tag="VF240_09015"
     CDS             complement(16567..17550)
                     /locus_tag="VF240_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX7175385.1"
                     /translation="MRERVAKLACAAAAVCALALSPAVAQQQQVQPRDAGAGLARREM
                     RRPKQTRDVTKAALIPGGTFEMGTDSADLARLQQIFGIRRAEVFSSEVPRHTVTLSPF
                     YIDRREVTNAQFKKFLDRHPVWRAGNVSPRLHNGNYLKHWSGGTYPDGRADHPVVNVS
                     WYAAVSYCRWVGARLPTEAEWEYAARGGLVGKEFPWGDDAPDKTRANFSASGVGDTTP
                     AGSYAANGYGLYDVAGNVWEYLADEWGAYASTPETNPVAGGNLFTVGDSFLGVTTRRV
                     LRGGSFGGAPVNLRVAYRDSHPPEGAQPFVGFRCARASRAQYRERKPRGSA"
     gene            complement(17719..19647)
                     /locus_tag="VF240_09020"
     CDS             complement(17719..19647)
                     /locus_tag="VF240_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175386.1"
                     /translation="MRDRFRRPLAALCLIALCAPAIFAQQPVGVQRKGTKKPAATPAA
                     AEADPLAEVRRATAIQIVNALADEARNFGDPSLRARAQGRAADVLWETDGERARLLFR
                     RAWEAAVAADEDNLRRDEEEKRRQMAERGHASYRPRPSIRTEVLRLAAKRDRALGEEF
                     LKMLEEAGRKEAGALVEGTTPPPTPEQRPDAQSTPPAVEKRLRLAVQLLQDGDVERAT
                     QIAEPGLGVVSVLAIDFLSRLRPHDAAAADARFSSLLQRAQSDPAADANTVSLLSSYV
                     FTPALFVTFTPDAGSNSSRYGEALPTPADLAPALRASFFNTASAILLRPILPAEQDQT
                     SAGRAGTYMVITRLLPLFEQQAPAAVPALRTKLAALTPDTPERSRQPGNNALTSGLVP
                     EDPNRDRTQETLGRLDRAKDSGERDAIYSDAALDAARRNDHQRAAEFVEKIEDLDARR
                     QLRSYLDYEALDAAVRDKKGAQEVLRLARASDLAPMHRVWGLTEAARLLAKDEPGRAA
                     EVLEEATAEARKLDHGTTGRVRALTAVVTQLHAVDRQRAWDMLAEIVKASNAAEGFTG
                     EDATLEAELRTKFGTTRRTSTAESFDLAGLFASLAKENLDRAVELARSFKSEHPRTAA
                     TLAVARAVLDRKDTRAAR"
     gene            complement(19647..21584)
                     /locus_tag="VF240_09025"
     CDS             complement(19647..21584)
                     /locus_tag="VF240_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175387.1"
                     /translation="MSHRRRAHTLRVLALCLTLSPAPLLAQRPAAGNQRNKPKPAPAA
                     ETDPVAEARRASAVALVVALADEARNFGDMALRAKVQARAADVLWETDAAKSRALFRR
                     AWEAAETADRDSGEERAREAGRRGVANSRDAGSVRREVLRQAARRDSAMGDEFLKRLD
                     ESSKQDAPAGAAPTAAGSTSAPSQRINPDDPPSSMTQRLNLASQLLQDGEVDRAMQFA
                     DPALYPVNTFGMNILNLLRERDAKAADQRFMSLLQRAVADSNSDANSVSLLSSYVFTP
                     FLYITVRPDGNSHTRQWRGNAAPPADLDPRLREAFLRAAAQILTRPLPPVEQDPTSAG
                     RTGAYVVATRLAPLFDQYMPDIAAQVRTRQAALLQETPERVRRPDNDLFTEGLVAKPS
                     REDELQNMLGRIENAKTPGERDRIYFRIAMGFAEDDLTRAREYAAKIEDIDTRKQLLG
                     ALAYRGLEIAIRNKRAEDAVRLAHADELTTVQRAWALTEAARLLTKDSPGRAVELLEE
                     ASVEAKRIDAGTADRARALTAVATRMHALDRQRAWDMMPEIVKASNAAPEFTGEDGQI
                     MIRVQFKGGGAMTNNNSVESFDLDGLFASLAKENLDRAAELARGFKEESARSAATLAV
                     ARAVLVKPRAGSQVDEREERN"
     gene            complement(21877..22449)
                     /locus_tag="VF240_09030"
     CDS             complement(21877..22449)
                     /locus_tag="VF240_09030"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX7175388.1"
                     /translation="MPKIDNIEETPNPNAVKFILKEPVSSSVARSFHSIEEAGADALA
                     RALFEVGEVASVFYMGDMITVEKKEDADWDDLLPRLAVPIRAAPSAAAGAPVAAAAAA
                     AGVGGALGALMSDDPRLLRINEILDERIRPALAGDGGWLEVLSLKENTLTIRYQGACG
                     SCPSSLSGTLVAIEGMIRQEVDPEIEVVAV"
     gene            22854..23999
                     /gene="bshA"
                     /locus_tag="VF240_09035"
     CDS             22854..23999
                     /gene="bshA"
                     /locus_tag="VF240_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEX7175389.1"
                     /translation="MNIGITVYPTYGGSGIVGSELGKELAARGHTVHFIASALPTRLT
                     ELNERVRFHEVEMMSYPLFEHQPYTLALATKMSNVAESEDLDLLHVHYAIPHSISAIL
                     ARESLKPHRALPVVTTLHGTDITLVGADRSYLPITRYGIAQSDGVTAISHYLARATAE
                     TFQFDHIAVIPNFICGSDYRRRPVPELRAQLAPNNEPLLVHVSNFRPVKRPVDCVEIL
                     ARVRERGVDARLAMVGDGSERPLCEYRARCLGVEEFCTFVGKQPRIVDYLSAADVLLL
                     PSEQESFGLAALEAMACEVPVVASRVGGLPEVVTDGETGCLAEVGDVERMADDAARLL
                     RDDAARREMGTRARASALERYSTDLVIPQYIEFYERVIAAAKEGRTV"
     gene            23956..24846
                     /locus_tag="VF240_09040"
     CDS             23956..24846
                     /locus_tag="VF240_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175390.1"
                     /translation="MSASSPRRKKAAPSEGPAAAPESARAWERAAGRHAAEDSRPTRP
                     TRPGAGGVRARAIRTAAPAHDSRPAAPAHDVRAPEKPIAYIVQNLERSYGVPVNKWGG
                     WDPLDMLVATILSQATSDINSERTYDALKRRFPNWDAVLRARQSTLADTIRQGGLANQ
                     KARVIRDLLRQVKERTGALKLDFLNDLPVEEAAQFLSSFRGVGPKTIACTLLFACQRD
                     IFPLDTHIFRVLRRVGLVPDRCSDARAHELMNGLVPKGKFYSFHVNLIRHGRRLCRPR
                     DPLCAECPIVEYCDYGQTVI"
     gene            25133..26080
                     /locus_tag="VF240_09045"
     CDS             25133..26080
                     /locus_tag="VF240_09045"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7175391.1"
                     /translation="MNCMNCGGSLTAGVKFCPRCGTPTPQPDPYGQQQQYVPPGGQMY
                     STFDTGAQQQQQYAQPRRKSRAGKFLLIFLGVILVLGAGAMAAAYFGYRYIEGTFKNS
                     EPYLLAVQELRRNPVAAEHFGEINDTGFPLGNFNESVGGTGHAEFSMSVEGEKGSGNY
                     MVTMSRTDGVWSVKNSVIRTPEGKLLNLSGKPSIIGSGDVGSPDPGAATNNSRGRANA
                     AAGAPAISGGVLNGKAVSKPAPPYPAIAKAVRASGTVTVQVTVDEEGRVREARAVSGH
                     PLLRAAAEAAAREARLTPTQLSGKPVKVTGVLTYDFKPE"
     gene            26119..26604
                     /locus_tag="VF240_09050"
     CDS             26119..26604
                     /locus_tag="VF240_09050"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7175392.1"
                     /translation="MAYLVDGNNVMGQRVGWHRDRPGARRRLLGDLARFARREEASLT
                     VVFDGAPDEHFPDGSDFMGVRVFYAERGRTADDRIKSMVDAARERRTLLVVTSDRQLA
                     DYVRRSGVRVQRSGEFRKRLDATQRREDAPEKADEPPPSHDELLREMRYFGVGPEDEE
                     Q"
     gene            complement(26674..27120)
                     /locus_tag="VF240_09055"
     CDS             complement(26674..27120)
                     /locus_tag="VF240_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7175393.1"
                     /translation="MPEAVKNGDTVRVHYTGRLETGEVFDTSEDGEPLEFTLGSGEVI
                     PGFEEAVRGMEVGGERTVEIAPDDAYGPRREQLVATVERENAEFPVEPQVGMNLNLPL
                     PEGNHLEVVVTEVTDTHVTIDGNHPLAGRKLIFDLKLIEKAVSSEQ"
     gene            27280..28149
                     /locus_tag="VF240_09060"
     CDS             27280..28149
                     /locus_tag="VF240_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175394.1"
                     /translation="MKNLTSPLVAALLALQPLVAPVALPHAASPATEIARETLSAQTS
                     APLLPTPTPAEAGDSDVVRITTNLVQIDAVVTDKQGRLVYDLGPEDFEILEDGEPREI
                     TNFSFVSAGAAAAGERTGSASQFVEVPNLGKERLALSGMVLMGVSSSSGSAANDAAPG
                     AADASPQQQSPAAEFDPDAVPAVRRFRQGASIEYYLHVYNAKLDPATGKPVFQYHARL
                     FRDAQQVFAGNPVNFNPGQQTDMKRLLVGGRLRLGAALPPGEYVLQVVVNDTLAKEKH
                     RMATQWIDFEIVK"
     gene            28233..30590
                     /locus_tag="VF240_09065"
     CDS             28233..30590
                     /locus_tag="VF240_09065"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX7175395.1"
                     /translation="MSLLFAATLFLSAALLFWVQPLVAKMLLPLLGGTPAVWNTCLLF
                     FQFVLLAGYAYALALTRWLSTRAQVFLHVALLIVAAVSLPVGLPGSAVEGVPEGGEPV
                     WWLLVTLAASVGLPFFALSATAPLLQRWFSKTRDPRARDPYFLYAASNAGSLVALVGF
                     PLVAEPNLRLAGQGRAWAAGYAALVLLLAACAFALLRSGPAEVNATAAKPRDEDDASS
                     DDADNDADTETLTKLRRLRWAFLAFVPSSLVLGVTTYITTDVAAVPLLWVVPLALYLL
                     TFVIAFSRGRLLDGRLADRLLPGAAILLTVVYLSGATEPAWFLVLVHLVFLYIAALAC
                     HRRLADDRPAPRHLAEFFLWVAVGGALGGLFNALLAPLLFDTIVEYPLAVVLACLARV
                     PFGARKSRTTPGAQRGDGGGAALDDSNELRREEDDARSREERRARVLDVLLPAAFGVA
                     VAVLTGVVTMLEMGSVERLAISLGLPLLIINHVFQRRPVRFALALGAVMLSTFLLTDG
                     GANTLHAERNFYGVVRVMQDASNDTLRLHHGSTIHGRQFADPARACEPLSYYHPTGPL
                     GSLFETFETRAGATAATSPHVAAVGLGTGAVVAYSRPGQSWTFYEINPEIVRLARDTN
                     AFTYLRDCARAPAVVVLGDARLQMRGAPESHYGLILLDAFSSDAVPAHLLTREALDLY
                     LSKLAPGGLIAFHVSNRGLDLHAVVGGLARDAGLAALVFDDPSFDNASGKEPSEWVVV
                     ARRPEDLSTLALKPNWQPLNESPRRRFAVWRDDFSDIISVFKWGG"
     gene            complement(30723..31628)
                     /locus_tag="VF240_09070"
     CDS             complement(30723..31628)
                     /locus_tag="VF240_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175396.1"
                     /translation="MRAPSLRPAAFLFVALAAQTTTAQKTAPPSPAQTQAVASYLSAV
                     EQHNRGRLAEAVDSFRRAAALDPNLSEAQAGLCVAASKLHLHEEALDACRRAVRLRPD
                     SAPAHFNLGGLYISLGMHEQAVASLKEVLRLDAASVEAHSDLGAAYHKLGRVAEAIAH
                     LKEAVRIRPDFSTGHGNLGVVYADTGRYEEAAASFRRALELDPASAELHYRRGDACSR
                     SGRRDEAVESFKQALRLKPDLALAHYRLGLLYAEAGDKKAALEQHRALQSHDPELATR
                     LFRRIHQDKLVTTPGGASASGGKLP"
     gene            32200..35607
                     /locus_tag="VF240_09075"
     CDS             32200..35607
                     /locus_tag="VF240_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7175397.1"
                     /translation="MSIKKWMLTALLLCACGASALAQTETGQITGKVLDPNGALVAGA
                     AATVKSVETGRERAATTNEEGVYIVANLQSGLYDVTVNATGFAPSTQRVQVTVGSQVS
                     VETALTISAIPGETVEVVAGGGVEVNTQTQELSTVVSGAQIRELPTVTRNPYNLVQLS
                     GNAATDDPTTSQNDTAGTATYRGAGASLNGLRAASTNILLDGADNNNSYTAQVGQSIP
                     LDAVQEFRVISSNFTAEYGRASGGIVNVATRAGSNQFHGTAYAFNRLSRLASNGFANN
                     ALGVERGVFTRNQFGYSAGGPVIKNKLFFFNSTEWTRVRSTGELTSFVPTSELIGLSA
                     ANTRDFFSAYNVSARPTGRTVSAADLGGISGIPGATPAFREVAFTVPTDLGGGFPQND
                     VSTVTRIDWNINDRTQLYGRYALQDQILFEGAGAFSPYEGFNTPTSQFNQNALLSLTR
                     TFSQNLVSTSKVAFNRVNSQNPLGAQPAGPTLYVFENVAAAIPGTATAIAFPGYLPFN
                     PGSAIPAGGPSNQLQFYQDFNYQRGNHQFRFGGNYINIQQNRTFGAFQNSVLTLGNTL
                     NQALNNLVAGQLLKFQGAVDPQGNFPGDFVTTPLGPPSFSRSNVYNEWAAYLNDSFRV
                     LPNVTLNLGVRYEYFGVQHNKNARLDSNFYFGEGATIQERIRNGSVQIAEDSPVGGLW
                     APDKNNFAPRLGIAWDITGDGRTALRGGYGVAYERNFGNVTFNVIQNPPNYAVVAVTA
                     GAEVDSIPLSGNNAGPLAGSGTTLRLPTTSLRNVRPDIRNAYAHFYSASFERELGRNT
                     VASVEYSGSSGRKLYSLENYNRVGAGPVFLGSNAVTPTGGASSRLNGQYSNANTRGNG
                     GYSNYNALILGFESNSFRSSGLQFTARYTLSSAKDNLSSTFSDSNANFNLGLLDPFDP
                     SLDYGYADYDARHRFVASFNYEVPYFDRLESKFARHVLGGFSLTGIFNARTGNPFTLV
                     DCTSGAGGLCHRYIPGGSAGISGSDNPRSVGANAFAYIDVPAGVVYTNPVAGNGALPP
                     FPAEMSARNAFRGPGYWNFDAGLYKRIRFSEKYALQLRLEAFNVFNHSNLFIAPGGAL
                     DLSQFHNFDADGNLINTQPGYATAQRGVFPSGNLERRNVQLAVKFIF"
BASE COUNT         5821 a        12287 c        11638 g         5866 t
ORIGIN      
        1 cttcgtctcc ctgaagcgcg agaacaggct cgggccttcc ggctcggacg gctgcggcga
       61 cgccagttcc tgcggcgcat ccgcgctcgc atagcccggc cgcgtgtcgg ggccaacgtc
      121 cgcggcggca aacgccgggg ccgccgccgc cgccgtgctc atgctgctac ggcgtgcgct
      181 gcgcgccgac ggcgagccgc cgagaatggg ctgcgaggca taggcgtgcg tggcggcggg
      241 gagcaggagt tgagcgtcgt cctgttcata ctcgtcctca tcgtagtcat cgtcgccgcg
      301 cccgaagagg gcgccgccga tggtgtagcc gaggagcaag cccgcgcccg cggcgccgag
      361 cgtgacgggc aacgtgtggc gccggtactg ctcgcgccag tcgagcgtgt cgctgatatt
      421 ctccctcacc tgctgatact gactcacgac tgactccttg atctccgtca cggtctgcgt
      481 gatcgattcg cgcgcttcgt ccatgcgacg ctgaagctct tcctttgtgg cgtcggggtc
      541 ggccgtcacg tcctcgggtg cgcgcgcggt cgctaaattt cgttcttcag ccattgcttg
      601 tcctttctaa tctcgtcgag actccggttc ggcgtcgggt cgtgcgcggc gaggcggccc
      661 ttcgccatca ggaggatgac ggccccggcc acgaggtaaa cgaggcccgt gatgacgaac
      721 ccgagggcgt agttgagccg cgggtcggcg aacgaaaaga gcttcgagac gaagaaggcg
      781 atggcgacgt taaagagcat gaagccgatg gccgccacca ggccgccaac ggccatcaac
      841 accccgttac gcgcgtagac ggaggcgtct tccttaagct cgaccttgat gaggccgagc
      901 ttggcatcca cgagcgtcat cacgtcgtcg ccgaggcgtc cgaacagcga cggcaggcta
      961 tctatctccg cctgtgtcgg ctcccgccgg gccggcgccg cgccggccgc acccctctca
     1021 agctgcgaca tatcgttcac ctccaagttc aaagttcaaa gtccaaagtt caaagacaaa
     1081 gctcgcagtc ggtttaagac tttgaacttt gaactttgaa ctttgaacct ttgtgttaac
     1141 ggcggctgcc gcgcagcagg atgccgagga ggaacccggc ggcggccgaa atcaacagcg
     1201 cctgaccggg attctgccgc gcgtactgct tggcctcgtc ggccatctgg ccgtagtcct
     1261 tgttacgcag gtcctggaac ttctcgccga ccacgccggc cttctccgtg acgtagtcct
     1321 tggccgtcga agccgcgtcg gcgatcttct ggccatactc ctgcgcctgc gaggcgagac
     1381 ccgtggcggc tccggcgccg ccgccggtcg aggtcgtcga tgagctgcgc gcgagcgagc
     1441 cgccgccgga gctggaggca gggccagaac cggaggtgga gccggaagcg gaaccagagg
     1501 tggaaccgga agcagaaccg gagctgggtt tattccctgc gtcggtcgcg cccgtgccgc
     1561 ctgtgtagcc ggtgcccgtg tggctgcttg atccggttgc gctggagccg ccggtcgtgc
     1621 ccgtcgtccc cttggagcca gtcgtggagt tcggtgaatc gttattgggt ttgttagctg
     1681 acatgtttat cgttctcctc cgcgtgtgaa atttatgttc gtagccgata ccgaagatgg
     1741 cgcgcaggtg gtcgcctctg cggacgttcg tcttttcccg ctgtgaatgt gcaaacgatg
     1801 tgccggcaag gcagcgcccg ccggcctcgg cgcgaaatgc gcgcagaacc ggaggcgcgc
     1861 gcgccctccg gctttctcca aacatcgctg ttttgttgcg aaaaggtacg cacagattag
     1921 cacaaggtcg ggaacaatac atagccggca cgcgccgagc gcactctctc ggagaaatac
     1981 gaaacccgct tgacagggct tccgtattgc ggtaagtaag tacttacttt taaaatccag
     2041 acagttgcgg agacgtgatt ttttagatga ccagacagcc agacaaagcc ggcgcgacgc
     2101 ccgccgccca aatccccgcg cccgcggccg ccgggcgcat ggccggcgga gaccgccgcc
     2161 ggcagatcgt tgacgtggcg atgcgtctgt tctccgagcg cggcttccgc ggcaccacta
     2221 cgaaggagat cgccacggcc gccggcgtct ccgaggccat catcttccgc cacttcgcca
     2281 ccaaggaaga cctctacacg gccatcatcg accacaaggc ctgctccggc gagatggccg
     2341 acatggagca gcccgtcgcc gaggcgatcc gctgctccgt ggccgagcac atgagcgcca
     2401 aggacgaccg cgccgtcttc gcgcacatcg cgctccacat gatgcagcac cacgagcgcg
     2461 acacggagtt cctgcgcctc ctgttctact cggccctcga agggcaccag ctctcgcgga
     2521 tgttctggga gcgaaacgtc cgcacgctct acgagttcct cggctcatac atccgtgagc
     2581 gccagcgcga cggcgccttt cgcggcgctg acccgctcgt cgtcgtgcgc gccttcgccg
     2641 gcatgatcat ccaccactcc gtgacgaaca cgctctggga caagtcgcgc tcgctcctca
     2701 acatcccgaa cgaggaggcg gcgcgcgaat tcactgagat acttctccgg ggcatcgacg
     2761 ccggccctgc gaaggccgcc cccgcgcgcg cggggcgggc agatgtcgcc gccatagcgc
     2821 gtgccagcgc aacctcgcgg cgcgcgccgc gcgccaaaaa gggcaagtag tttttccata
     2881 ccgaaagggc caaccgggta aggtcatgtt ctgccttcgt caactccaaa ctttcacgct
     2941 cgccgtcgcc gtcgccgggt cgggcctcgc tgccgcggct tgcggcggct cgcgcgcaga
     3001 gaccaataag aaagaaggcg gcgcggccac gcaggcgacg cccgcggccg tggacgtgac
     3061 gaccgcgccg gccgtcacgc gccagctccc gcgcttcatc gaggcgacgg gcagcctcgc
     3121 ggccgatgag cagacggacg tggcgcccat cgtcggcggc cgggtctcgc aggtctacgc
     3181 agacctcggc agctacgtgc agcgcgggca ggtgctcgtg cgtctcgacg gcgaggacgc
     3241 gcgcctgcgt ctccagcagc tcgaagcgca gctcgaacag gcccgctcgt cagtgcgtca
     3301 ggcggaggag cgcatcggcc tgcgcgccgg cgcgcgcttc gacccgacgc gcgtcgccga
     3361 ggtcggggcc gcgcgcgccg cactcgatct ggccgagaag cagttgcgcc gctacgagcg
     3421 tctgatcgag tcgggcgacg tgtcgcgctc cgcatacgac cagcagaagg cgcaacgcga
     3481 ccaactgcga cagcaatacg aagccgcgct cgcgcaggcc caccagtctt acgccggggt
     3541 gcagacggcg cgcgccgccg caatggccgt ggcctcgcag gtcgagcagg cgcgcaaggc
     3601 cgtgcgcgac gtggtgatcg tctcgcccat cagcgggttc gtctccgagc gcacggccga
     3661 cgtgggcgag ttcgtctcca cctcgatgaa gatcgccacc atcgtccgca ccaaccccct
     3721 gcgcgtgcgt atcgacatcc ccgagcaggc catctcaaac gtccagacgg gccagagcat
     3781 cacgcttctg acgagcgcct accctgaccg cgccttcgcg ggacgggtcg cgcgcatctc
     3841 gccgaacatc tcggcggcct cgcgcacgat gacggtcgag gccgaggtgg agaactctgc
     3901 ggggctgctc aagccggggc agttcgtcac ggcgcgcatc cttctgcccg agtccgaccc
     3961 ggccgtgctc gtacccgtcc gcgccatccg cacggacggc acgaccagcc gcgtcttcgt
     4021 catccgcgac ggcgtggcgc aggagcgcct cgtgcagacg ggacaacagg agggcgacct
     4081 cgtcgaagtc cgcggcaacg tcgcggcgga cgaacccgtc gccacctcga acgtggagct
     4141 attgagcgac gggatgggtg tcagacagtg acttgtgagg cccgcgtggg aattacgggt
     4201 ttaaggcgtc gcgggaaaat gctcccggaa ctgtgttcgc acttcgcgga tgtcggcgcg
     4261 cagctcaagt atgtcgccgc gcgtggcgtt gacgaccgtt tcgaggcggt cgaacctaat
     4321 gtccaggcgg tcgatcttgg catccacgcc gtcgatccga gcgttcaggc gctcttcgag
     4381 cgtcccgaga ttgaggtcaa atctctcttc gagagaattg aacttggtgt ccatgcgctc
     4441 ttcaaaagag acgagacgtt cgagcacggt ctcgatggtc ggcttagtta ctttttcgtt
     4501 actcatcggt cttgtacctc cgatttacgg ctggtgagga gcggctgaat tatacaacaa
     4561 ggcgggcggg ccgtgtcact gtcgccgaga cggaggacgg cccggccgcc gaaccaaaac
     4621 cagaagcgcc atgcaaaagc ttgctgaaat ctgcgtccgc cgccccgtct tcgcgacgat
     4681 gattattttg tcgctcgtgg tcgtcggcgt cttctcgttc ttctcgctcg gcgtcgatct
     4741 gtttccgaag atcgacttcc cgacgatcac cgtgacggtg gtcaaccccg gcgcggggcc
     4801 gcaggagatc gagaccgaga tcaccgataa ggtcgagtcg gccgtcaaca ccatcagcgg
     4861 catcgacgag ctgcgctcga cctccgtcga gggcgtctca caggtcttcg tcacgttcgt
     4921 cttagacaag gacgccgacg tggccgcgca ggagatacgc gacaaggtca acctcatcgt
     4981 ccccgacctg cccgagacgg ccgaggagcc gaccatccaa aagctcgaca cggacgccgc
     5041 gcccgtgttg cgcctcgtcg tctcggcgcc gcgctctttg cgcgacgtga cggacgtggc
     5101 tgacaagcag atcaagaagc gcatcgagtc gataggcggc gtcgggcagg tgcagatcat
     5161 cggcggcgcg cggcgcgagg ttcaggtcaa ggtcgatccc gacaagatgc gcgccttcaa
     5221 cgtcacgccc gccgacgtgg tggcggccgt ccgccaacag aacctcgaag ttccgggcgg
     5281 gcgcgttgac gagggcgcgc gcgaactcac cgtgcggacg atgggccgca tcacgaaccc
     5341 ggccgatttc aacaacatct cggtcggctc gcgcggcggc tacccggtga aaatctccga
     5401 catcggctat gtcgaagatt cgagcgagga gcagcggacg gccgcgttcc tcaacgggca
     5461 gccggccgtc acgctcgtcg tctcgaagca gtcgggacag aacacggtgg ccgttgccga
     5521 cgccatcaag gagcggctcg ccgacattca gccgacgctg ccgaaagacg tgaaggtcga
     5581 ggtcatcggc gacagctcga tcttcatcaa ggcggcggtc gagtcgattc agacgcacct
     5641 catcgagggc ggcatcctcg cggcgctcgt cgtcttcatc ttcctctgga acttccgctc
     5701 gacgatcatc gccggcatcg ccatcccgac ctccatcgtc gccaccttcg gcctgatggg
     5761 cgcgatgggc tacaccctga accagatcac gatgctggcg ctcacgttga tggtcggcat
     5821 cgtcatcgac gacgccatcg tcgtgctgga gaacatctac cgctacatcg aggagaaggg
     5881 ctacgagcct tttcaggcgg ccatcgaggg cacgaaagaa atcggcctcg ccgtgatggc
     5941 gactactctt agcttgctcg ccgtctttct ccccgtcggc tttatgaagg gcatcgtcgg
     6001 ccgcttcatg tcgagtttcg gattcacggc ttcgttcgcc atcgccgtca gcttgctcgt
     6061 ctccttcacg ctcacgccga tgctctcggc ccggctcatc aagcgccggc gcgaggagca
     6121 ccgcgaggcc gaggcggtga cgaacgacga tcaggtgcaa agcgtttcgc tctcggaaga
     6181 ctccgggccg acgcccgaag tgccggcgat gcccgccgag gtgcccgcca ctgatgaggc
     6241 gcgcagcgcg aagacggcca aagactcgcg cttctaccgt cccatcgacc gcacctacac
     6301 gtggctgctt cattgggcga tggcgcaccg ctgggccgtc gtcaccctct gcgtgctcgt
     6361 cgtcctgagc acggcgccgc tctttatgct catcggcaag aacttcctgc ccgtcgatga
     6421 ccagtcgcag ttcgaggtca cgctgcgcgc gcccgaaggc tcgacactcg cctcgacgaa
     6481 gaccgtcacc gagcgcatcg ccaaagaact gcgcgagctt cccggcgtca ccgacacttt
     6541 aacgaccatc ggcggcggcc agcagcagct cgtcaacagc gccaacatct acgtcaagct
     6601 cgtggacatc ggcgaccgca agcagtcgca gattgaattg caggacgagg cgcgcaagat
     6661 tcttgagaaa tatccgaagg agctgcgacc ggccgtcgga caggtgcagg ccttctccgg
     6721 cggcggcttc cgcaacgccg acgtgcaata cgtcgtcagc ggccccgacc tggagaagtt
     6781 gaccgactac tcgaaccgct tcctcgaaaa gctacaggcc atccccgacg tggttgaccc
     6841 cgacacgtcg ctcatcaccg gcaagcccga ggtgagagtc cacatcgacc gcgcgcgcgc
     6901 cgccgacatg ggcgtgcgcg tcggcgacat cgcgcaggct ttgaacgtct tagtcgccgg
     6961 gcaggtggcg acgacctaca atcagggcac ggatcagtat gacgtgcggg tgcgcgccac
     7021 gggcgagtcg cgcgcgagcc agaaggcgct ggagaatttg ttcgtcccgt cggcgaagac
     7081 ggggtggacg aacctttcga gtctggtgcg gacggaggag gggacggggc cgtcggccat
     7141 cgagcgcctc aaccgacagc ggcaggtaat catctcggcc aacgtcaaac ccggcggctc
     7201 gcaggcggcc gtgacggcgg ccctcgacga attcgcccgc gagatcaacc tggaggccgg
     7261 ctacaccacc ggcaccttcg gccgctcgaa ggagctacag aagtcgggct attacttcat
     7321 gctcgccttc gcgctctcgt tcatcttcat gtacatggtg ctcgccgcgc agttcgagtc
     7381 gttcatccac cccatcacga ttttgctgac gctgcccctc gccgtcccct tcggcattct
     7441 ctcgctgctc gtgatggggc agacggtgaa catcttctcg ggcctcggtc tgctgctgct
     7501 cttcggcgtc gtcaagaaaa acgccatctt gcagatcgac cacacgaacg cactgcgctc
     7561 gcacggcatg gagcgactcg acgcgatcat tcaggcgaac cgcgaccgcc tgcggcccat
     7621 cctgatgacg accatcgcgc tcgtcgccgg catgatcccg ctcgtcatct cgacggggcc
     7681 gggcgcgggc accaaccgct ccatcggcgt cctcgtcgtc ggcgggcaaa gtttgtgcct
     7741 gctgctgacg cttttggccg tgccggtctt ctactcgctc ttcgacgacc tctcgcacag
     7801 ccgcgtcccg gcgcgcatcg gccggcgcgt gggcggcgtc ttcgcgtggg cgcgccgacg
     7861 cgcggcgacg gcgacgagtt cgttcttcgg catcgtcggc aaaaattgat ttgatagggg
     7921 cggcgcgcgc acgcggattc attccttata gacctccgcg tccgcgccgt ttaataaagt
     7981 tatgagcaaa cgatttcgta aattgacgtg caggctgacg gcgctcgcct tgctggtgtg
     8041 ttcttccgcg ccggtcggcg tgctggctca acaaacggcg ggcgcgaggg cgcgacagca
     8101 acagcagcaa caacggcaac aaggtgagcg gcgcgaggag ccgcagaagt tcaccggcac
     8161 actcttcggc ggcgacgcct cggccgagac ccgcgaagcc gagccgcgcg aagggcgcga
     8221 ggttgaggcg gcgggtgtcg ccacgccggc cgcgcgcgtc gtgaagaaag acgtgacgcc
     8281 gcagtcatac gtgcgtgtca cgctcaagcc cgcgccgcgc tacgaagagg ccgcgctgca
     8341 tacggcgacg gcacagcagc cgcagcaggg acagcagacc gcttcgcccg ccgcgccttc
     8401 ggccaacaac ccgccgccca cgcagggcgc gccggcgcag gggccgcaga ccgcctcgcc
     8461 cacgcagtcg cagacacaac aatcggcgaa ccccgccgcg cccgcgcagg gtcaacagca
     8521 acccgcacaa cccgccgtgc cgccggcctc gccgacacaa ggcgtgtcca cgcaaacaca
     8581 aggtgaaggc acgcagcccg cgcaaggtca gccgggtcag ccgcaacagc caagcccgaa
     8641 cgctcccggc tcgcaggtgg gcgcgccgca ggggccgcgc gcgcccggcc gtgagagtgt
     8701 gccgatggcc gcgcccgccg acgaagggct acaggtgccg caggtggcca cggacttccg
     8761 ctccgcggag gggccgttcc ccgcgctcga acgcgtcggc gtgcagatgg gcgaccagcg
     8821 cgcactctct ctgcgcgagg ccatcgagct ggcgctcgcc aacaacaagg acatcgaggt
     8881 cgcgcgcacg aatgttcgcg ccgccgagtt cgatcttcag gcggcgcgcg gcgtctacga
     8941 cccgcgcttc ctgaccaact cctactacga gcgcagcgaa actccggccg cgagtttcct
     9001 gagcggctcc acttcgggcg cagtcacgca gtcgggcttc ttcagcaaca cttcgtttca
     9061 ggggctcgtg ccgaagtacg gcggcggcta tcgcgtcgat ttcgtctcga accgcgtgac
     9121 gaccgacaac cagttcgccg cgctcaaccc gcagtacccg accgcgctca ccttcagcta
     9181 cacgcagccc atcctgcgcg gccgcgcctt cgaccagaat cggcgcttca tcgagatcgc
     9241 cagccgcaac ctgacgctca ccgacgcgca gttccgacag cgcgccgtcg agaccatcac
     9301 gcaggtgcag cgcgcctact gggatttggt cttcgccctg cgcaacctgc agattcagcg
     9361 cgacgccgtg cgcgacgcgc gcgcgcagct cgaacacaac cggcggctcg tcgtcgaggg
     9421 cctgctcgcg cccatcgacg tggtcgccgc cgaggcgcag gtctccggct tcgagcagag
     9481 cgtctacacg gcgctcgacg aggtgggccg ctccgaaaac gtcttgaaga atttgattgc
     9541 cgagaaccgc gaggcggcat tgtggaacgt cgcgctcgtc ccggccgacc cggtggagct
     9601 gcgtccgcca acggtggact tgcaggccgc gatggattcg gccctcaaga gtcgccccga
     9661 aatacagacc tcggacgtgg ccgcgtccat caacgaggtc gaacagcgtt tcgcgcgtga
     9721 gcaggccaag ccgcagatcg acttcgtcgg ctcatacggc atcgtcggac tcgccggcac
     9781 gctcaacacg tccgacgcgg ccaacccgtt cacggcttcg agcacgcaac tgcgagagcg
     9841 catcaaccag ctcgcggcgg ccgtcaacgg gctgacgggc cgcaacgaca tcgtgcccat
     9901 cccgccgccc gcgccgcaag tcttgccgga cgtgctcgtc ggcggctacg gccagtcaat
     9961 cgccaacctc gccgccaacc gctacaacaa cttccgcgtc ggcgtcacgg tcaacctgcc
    10021 catcggcaac cgcacggccc gcgcacagtt gggccgcgcg ctcgtcgagg gcgagcgcat
    10081 cggcacgcag cgcgagcagt tggagcagct cattcaggtg gacgtgcgca actctctcca
    10141 gacggtacgc accgccgagt cgcgcctgcg cgcggcggcc tccgcccgct cggcctccga
    10201 gcagcagtac gcgagcgaga agcgcaagtt cgacgccggc cagtccaccc tcttcctcgt
    10261 cctcgaacgc cagacctcgc tcaccacggc gcgcggcaac gagctgcgcg cgcagaccga
    10321 actcaacaaa gcaatcgccg agctgcaacg cgccaccggc aacgccctcg aagccaaccg
    10381 cgtcgaggtg cgtgtgcgct aacgccggcg ctcggcgttt atcggaggcg cgtctggatg
    10441 gaagcgcaac gggcggagat tctcaatcga ctttcggcgc acgggtggag cgttcgctgc
    10501 gtggacgact atgagcttga gtggtgggcc gacgagatgt ggctactcga atcgacttgg
    10561 tctccggccg gcggccgtgc gtgggtcacg tttcttgtag acccgcagac ttcagatgtg
    10621 gcaaatcggc ggaagggtaa gggcgtgtgg gcgttgttgg cgtcgcccga aaagccgacc
    10681 cggtggcagg cgtcggaagg ggagttctcg ctgggtctcg ggcgcggctg ggaagatgag
    10741 ttacccgcgc tccttgagta tctggacgcc gtccgcgcgg ggagtcgtca ggatgcctga
    10801 gtcgttcagc acgaagctcc tgcgctggaa gttcaacctc ttcccggcct accgcgggac
    10861 gggcggccgc atcacttaca tcgcggcgga cttgcgcgag gtgcgcgtcc ggctgccgct
    10921 caactggcgg acgcgcaact acgtgggcac catcttcggc gggagcctct acgccgccgt
    10981 cgatcccatc tacatgctca tgctcatctg ggcgctcggc cctggctacg tcgtctggga
    11041 caaggccgcc tccgtgcgct tcaagcttcc gggccgctcg acgctccacg cgcacttccg
    11101 ccttgatgaa gacgaactgc gggccatcca gtctgagttg gagaacgcgc gctcgattaa
    11161 ccgcgtctac agcgtcgatc tcaaggacgc cgaaggcaag gttcacgcga cggtcgagaa
    11221 gaccatctac attcgtcgcc gggacgcggg cagtaaatcg aaggacacgg gcggccgtgc
    11281 cggcgagtgc tactgagtcg agggcgttgt cttcgccgtc ttctccagcc gcgccctgac
    11341 ccacagcggc cagtggtcgg agagaccgac cttcttgtcg atgccgtggc cggcgctcgt
    11401 gaggccgcgc aaccagaccc agtcgagctt gaagtcgaag aagacgacct tgaaggtcgc
    11461 gtcttcgtca cggaagggcg tggcgaagcc ggcctcggtg aagagcttgc gcgcggcgcg
    11521 cacgtccttg tccttgatgg tgttgaagtc gccggtcacg acgacgtgtt gaatcttcgg
    11581 gtggcggcgg agatcatcga gcacgatgcg ccagtgatcg actttcttct cgaccggcat
    11641 gcgcgtctcg gcgtggacgg agtagaagcg cacgtcgtcg cggccgatgc ggacggtcgc
    11701 cccgatcccg gcgcgacgcc ggcggttcgg cccctcgtgc ggcagcacca gacgctccac
    11761 gtcggagagc gggtacgggc tgaggatggc gacgcccgtc tcctcctccg gctccttctt
    11821 cggcggcttc gcgccgtcct tcggcggcgg cggcggcgcg gcccacgcgt agtgcatgcc
    11881 gagcgcctcg gccagaagac ggggcgtgtt ggcacgcccg gtgcgctcct tgttgcggtc
    11941 aacttcctgt aagccgatga gcgcggcccc gccgatttcg gggtcgtcct tcaaaagccg
    12001 aatcaactcc tgcaagtctt cgccgtcgcg gtaacggatg ttgtaggaga cgatcttgat
    12061 ctccgcaggt tcggccggcg gcgcgacgag cttgcgcgcg ctcccgactt cgaggagcga
    12121 agcatcggcg gcgcgctcgt ccgactcgga aggcgagggc ggtgtgccgt gcgcggaggg
    12181 caataagaag acggcgatga cgagaacaga cagcagcagg ccgagcgcga cgcaacgccg
    12241 acgcactgcc gacggtaggg acacgcgcgc gcccgccgaa gtcgtcacgg actgtgtgcg
    12301 gccagatata gacgacagac tttcgtcctg ccgacttctg acttctgact tctgacttct
    12361 gactcctgtt tctcgttctt cgctcaagat gattctcctt gcgtcgtgta gggcgcatcg
    12421 cccgcgagga agcgtgcggc gcgcgcttcg acccgatagc ggcagcgatc tgtctccagc
    12481 acctcgccgt cggtgttgac cttcacagcg cggtcgaagt cgatctcgac ctcgcgcgcg
    12541 cgcagataga tgacgcgctc gtcctcgacg tgttcgcccg aggcgatgct cgtcagcagt
    12601 ccgataaact ccagcgtcgg catcgcctcg acgatacaga ggtcgagcag cccgtcgtcg
    12661 atgagcgcgt cgggcgcgat gggtcggccg cccccgacca tgcgtgagtt acacgcggcg
    12721 aacatctgca cctgcatctc cctctcgaac gcgccggccc cgtcgtcgcc gatgccgcgg
    12781 gcgcgcacgc gggcgtgcgg cggctcatag tcgaagagca cctgcgcccc gccgatgaga
    12841 taggcgagct tgccggccag gctcttgagc gcgggatcga cggcgtccga gacctcggcg
    12901 atgaagccgc cggccgagac gttgacgaag cgtcgctcgc ccaacgcgcc cacgtccacg
    12961 cgcacttcgc gtccttcgag caaaacgtca acggcctctt ccacactctc cgggatgccg
    13021 agcgcgcgtg cgaagtcgtt gcccgtgccc agcgggatga ggccgaagac cacgctttcg
    13081 agcgcgccgg ggacgcccgc cacgccgttg agcgcctcgt tcaatgtccc gtcgccgccc
    13141 gccacgaaca gttgttcgta gcccgcgccg actgcctcgc tggccgcgcg cgccgcgtcg
    13201 ccctgcgcga ccgtcatcac gatgtccatc tcgcccacgc ccgcgcgcag ccgctcgtta
    13261 atcacccgca actgctccgg cgcctcgtcc gtccccgaga ccgggttaac aatcaaacgc
    13321 gatttcatta atagccccct tgtccgtgtt ctcagccgcg aagcggcggc gagaaggtag
    13381 ccagacgtgg aacgtctgga acagaccgac agcccgaccc gcgccttgaa gaggcgcgca
    13441 gactccggcg ccccttcagg gcgcgcaagg ctcgccccat cgccgcctct ggctaccttc
    13501 tcgctgcgcc tccggcgcaa agccaacgag ttgtcaaacc gcttctgacc actgacggcc
    13561 tttcacacct ccagccttag cccgtcgcgc gcctcaatcg tctcgccgtc ccagaatttc
    13621 gcggcctcgg cggcgaggtc gaggtcgtcc cactcggggt agagatgcga gagcacgagc
    13681 cggccgggcg cggcgcggcg cgcgagtcgt atcgcctcct caagtatgag gtgcgtctcc
    13741 agaggcttcg cgcggaagaa agagcactcc aggagaaaga gttttacgcc gcgggcgaag
    13801 tcggcaaccg cgtcggcgta gcccgtgtcg gaagtgtaag caagcgacgc gccgtcggcg
    13861 gccgtttctt cgagacgcag cgcgaggctt tcgcttgagt gcggcgtcga gaacgtggcg
    13921 gccccgacgc ccggcagaat ctcgaactcg gcgcgcgggg ccacctcgcg cacttcgacg
    13981 gggaagggct gctcgaagag tttgtaattg cccgcgccgt cgaacgtctc caaaagtttt
    14041 ctggcgccgt gggggccgaa gatgtggagc ggcttgcggc gcgcgcgcgt ctgcggggcg
    14101 tgcttcgtcc cgaagagaaa gggggcgagg ccgccgacgt ggtcgaggtg gaagtggctg
    14161 acccagacgg cgtcgaggcc cggccagtca agcccctcct cggcggcgcg gtggaaggcc
    14221 gtcgcgctcg ggtcgagcag cagtgtgccg ccccgcgtct ccagccagtg tgcggaagag
    14281 gagcggcgcg ggtgcgggac agccgtgccc gagccgagta ccgtcagctt catcgagatt
    14341 agtccttcag ggggaaagcg cacgttgagg ctaccacact cgcgctcccg cgcgtcaacg
    14401 cccgcttgac cagccgacgg cgcttgcgct acatcttcac ctgaagttcg ccgagaagcc
    14461 gagccacgag cgccggaggg acgaggcggc ctctttaccg aaatgcacgg cgttgtttca
    14521 ctgctggacg acgagcacta ccgcttggtc gaagacgttt gggcggagct gtcacgcaag
    14581 ttcggcgtgc acggcatcta ctcgacgccg ttcccgcact tcacctatca ggtctccgag
    14641 gagtacgaca cgaagacggt cgagtcggcg ttgcgcgcgg tggccgaagc gagcacgccc
    14701 ttccgtgtca gaacggcggg cctcggcgtc ttctgcgccg cgcgtcccgt gctccacgtc
    14761 cccgtggtgc gctccccgga gcttgagagg ttccacgacg aggtgtggcg cgtactgggg
    14821 cgtccgcccg cgggcgaggt ggcccgcttc tacgactcac agatgtgggt gccgcacgtc
    14881 acgctcgcgc agggcgacat cgactccgag cgtctgggcg agatcgtgcg ggtgctggcg
    14941 gcgcgcagct tccactggga gatggaggtc aacaacctct cgctcatcta cgacaccggc
    15001 acggagcagg gactccgctg ccgcttcaac ttcggcaacg ggcgcggggc tgttgcgtga
    15061 gaccgggacg gccgtcgcgc gttcacgccg cgtctttccc tgatcttcaa cgggcgaccg
    15121 ggagagcgcg ccgaggtggg aaacttcaaa tgagtaacga agacgacagg gaaggcgccg
    15181 ccgcgttgcc ggtgcaggtt tacatcttca atcagactta caacctgcgc tcgggcagcg
    15241 atgaggaata cgtccgccgt gtggcgcaac gcgtcgatga gcggatgcga cagatttcct
    15301 ccatggcccc gtaccacgac ccgatgaggg tcgccgtgct ggccgcgctg cacatggccg
    15361 acgagctgga gcagtcgagg gagacggcgc aggagccgga ggacgcgcgg ccggaagaag
    15421 aagaaaccgc cgcctcggca gcggcgacgg ccgggagcgc gcccgatgct cgtgcgggtc
    15481 ttgagggcgc ttcctcctgg ttcgagagcg tcttcgattc ggagttcacc ggcaaccgca
    15541 gcagcggcga gcgtctgagc gaccgcgtct ccgaacgact ccagacgcgc cggcaggagc
    15601 gacgccgccc catcgtcatc gacgccgacg acacagacgg cacgccggcc accgaccgtc
    15661 gtgccgacgc cggcccacgc gataatcagt cacgcgatta agtttgattt gaagtcttcc
    15721 gacctttgtc tatgaccgat caacctgtgg aattggacgt gctcgccgtc tactcgcacc
    15781 cggacgatgc ggagcttgcg gccggcggga cgctgctcaa gctcaagcac ctcggccacc
    15841 gcacgggcgt gctcgacatg acgcgcggcg agatgggcac gcgcggcacg cgcgagatac
    15901 gcgagggcga ggcccgcgag gccggacgcc tcctccaact cgacgcgcgc ctcaacctcg
    15961 accagccgga cggccacgtc tggcccaccg aggacgcgcg ccgcgcgctc gtccgtgtcc
    16021 tgcgcgcctg ccgccccaag gtcatcctta ccgcgcactg ggacgacccg caccccgacc
    16081 acgccaacac ctgccgcatc gcccgcgagg ccgcgcgtct ggcctccatg cgccgctacg
    16141 acgaagagca cgggcaagcc gcgatcaaga tgccggccct cgcccactcc gtctactcgc
    16201 gtctcgtcgt cgcctccttc atcgtggacg tgtcggactt cgtcgaggac aagatggcgg
    16261 ccgtgcgcgc ccacgcctcg cagttctgga gcgaaggttc gaaggagcct tcgacgcgca
    16321 tcagcgacga gggcttcctc aaccagatcg agttccgcct gcgctactac ggctcgctca
    16381 tcggcgtcgc cgccggcgag ggcttctacg tccgcgaggc gctcaacgtc gaagaccccg
    16441 tcgcgctgct cacccggccg atgaacatct actcatagag cgactgctca taaagcgact
    16501 gcgtgcacgg cacagtaccg cgagcggtag cgagcgggtg cagcgacgac gagaggcccg
    16561 cctcggtcac gccgacccgc gaggcttccg ctcgcggtac tgtgctcgtg atgctcgcgc
    16621 gcaacggaag ccgacgaagg gttgcgcgcc ctcgggcggg tggctgtcgc ggtaggcgac
    16681 gcggaggttg accggcgcgc cgccgaagct tccgcccctg agcacgcgcc gcgtggtgac
    16741 gccgagaaac gagtcgccga ccgtaaagag attccctccg gccaccgggt tcgtctccgg
    16801 cgtggacgca tacgcgcccc actcgtcggc cagatactcc cacacgttcc ccgccacgtc
    16861 ataaagtccg tagccgttcg ccgcgtagct gccggcgggt gtggtgtcgc cgacgccgct
    16921 cgccgagaag ttggcgcgcg tcttgtcggg cgcgtcgtcg ccccacggga actctttgcc
    16981 aacaagccct ccgcgcgccg cgtactccca ctcggcctcc gtcggcagtc gagcgccaac
    17041 ccagcggcag taggacacgg cggcgtacca gctcacgttc accacggggt ggtcggcccg
    17101 cccatcggga tacgtgcccc cgctccagtg tttcagatag ttgccgttat gcaggcgcgg
    17161 ggagacgtta ccggcgcgcc atacggggtg cctgtcgaga aacttcttga actgcgcgtt
    17221 cgtcacctcg cgccggtcga tgtagaaggg cgagagcgtg acggtgtgtc gcgggacttc
    17281 cgaggagaag acctccgcgc gtctgatgcc gaagatttgt tggagacgcg caaggtcagc
    17341 ggagtccgtg cccatctcga acgtgccgcc gggtatcagc gccgccttcg tcacgtcgcg
    17401 cgtctgtttc ggccttcgca tctcgcgccg cgcgaggccc gcgcccgcgt cgcggggctg
    17461 cacctgctgc tgctgtgcga cggccggcga cagcgcgagc gcgcagacgg ccgccgcggc
    17521 gcacgctaac ttcgcaactc tttcacgcat gaacgacaac ctcaaagccg tttgtgtgta
    17581 cttcagacgg agcacaccgc cgccgcacgt ttcatggcag atagtttgag gtgggttgcg
    17641 acacgccggc agcacgactc gtgctgccgg acgtgacggc ccggcggcgc gtggcgaaag
    17701 gacttgcttg gggccgcctc agcgtgcggc gcgcgtgtcc ttccggtcaa ggacggcgcg
    17761 ggcgacggcg agcgtggcgg cagtgcgcgg gtgctcggac ttgaaggagc gcgcaagctc
    17821 cactgcgcgg tcgaggttct ctttggcgag cgacgcgaag aggcccgcga ggtcgaagct
    17881 ctcggccgtg gaggtgcggc gcgtggtgcc gaacttcgtc cgcagttcgg cttcgagcgt
    17941 ggcgtcttcg cccgtgaagc cctcggcggc gttcgacgcc ttgacgatct cggcgagcat
    18001 gtcccacgcg cgctggcggt caacggcgtg caactgcgtg acgacggccg tgagcgcgcg
    18061 cacgcgcccg gttgtcccat ggtcgagctt gcgcgcctcg gcggtcgcct cttcgagcac
    18121 ttcggcggcg cggcccggct cgtccttcgc aagcaggcgc gcggcctcgg tcagccccca
    18181 cacgcggtgc atgggcgcga ggtcgctcgc gcgcgcgagg cgcaggactt cctgcgcgcc
    18241 cttcttatcg cggacggcgg cgtcgagcgc ctcgtagtcg aggtaggagc gcaactgccg
    18301 gcgcgcgtcg aggtcttcga tcttctcgac gaactcggcg gcgcgctggt ggtcgttgcg
    18361 gcgggccgcg tcgagcgcgg cgtcggagta gatggcgtcg cgctcgccgg agtctttggc
    18421 gcggtcgagg cggccgagcg tctcctgcgt gcggtcgcgg ttcgggtctt cggggacgag
    18481 gccgctggtg agcgcgttgt tgccgggttg gcgcgaacgc tcgggcgtgt cgggggtgag
    18541 cgccgcgagc ttggtgcgga gcgcgggcac ggcggccggc gcctgttgct cgaagagcgg
    18601 gagcagccgg gtaatgacca tgtaggtgcc ggcgcggccc gcgctcgtct gatcctgctc
    18661 ggcggggagg atgggccgca ggaggatggc gctggcggtg ttgaagaagg acgcgcgcag
    18721 ggccggcgcg aggtcggcgg gggtgggcag cgcctcgccg tagcggctgc tgttcgagcc
    18781 ggcgtccggc gtgaaggtga cgaagagcgc gggcgtgaag acgtaggacg agagcagcga
    18841 cacggtgttg gcgtccgcgg cggggtcgct ctgtgcgcgt tggagcaggg agctgaaacg
    18901 agcgtcggcc gcggcggcgt cgtgcgggcg caggcgcgac agaaagtcga tggcgaggac
    18961 gctcacgacg ccgagtccgg gttcggcgat ctgtgtcgcg cgctccacgt cgccgtcctg
    19021 caagagctgg acggcgaggc gcaggcgctt ctcgacggcg ggcggcgtgg actgcgcgtc
    19081 ggggcgttgc tccggcgtcg gcggcggggt cgtcccttcg acgagcgcgc cggcctcctt
    19141 gcgacccgcc tcctccaaca tcttgaggaa ctcttcgccg agcgcgcggt cgcgtttagc
    19201 ggcgaggcgc agcacctctg tgcgaatgct cgggcgggga cggtaggagg cgtggccgcg
    19261 ctcggccatc tggcggcgct tctcctcctc gtcgcgacgc aggttatcct cgtcggccgc
    19321 gacggccgcc tcccacgcgc gccggaagag caggcgtgcg cgctcgccgt ccgtctccca
    19381 gagcacgtcg gcggcgcgcc cctgtgcgcg cgcgcgtagc gatgggtcgc cgaagttgcg
    19441 ggcctcgtcg gcgagcgcgt tgacgatctg gatggcggtc gcgcgtctga cctcggcgag
    19501 cgggtcggcc tcggcggcgg ccggcgtggc ggccggcttc ttcgtcccct tgcgctgcac
    19561 cccgacgggc tgttgcgcga agatggcggg cgcgcagagc gcgatgaggc agagcgcggc
    19621 taacgggcgg cggaagcggt ctctcatcag ttcctctctt ctctctcatc gacctgtgag
    19681 ccggcgcgcg gcttgacgag cacggcgcgc gcgacggcga gcgtggcggc cgagcgggcc
    19741 gactcctcct tgaagccgcg cgcgagttcg gccgcgcggt cgaggttctc tttagcgagc
    19801 gacgcgaaga ggccgtcgag gtcgaagctc tcgacggagt tgttgttcgt catcgcgccg
    19861 ccgcccttga actggacgcg gatcatgatc tgcccgtcct cacccgtaaa ctcgggcgcg
    19921 gcgttcgacg ccttgacgat ctcgggcatc atgtcccacg cgcgctggcg gtcgagcgcg
    19981 tgcatgcgcg tggcgacggc cgtgagcgcc cgcgcgcggt cagccgtgcc ggcgtcgata
    20041 cgcttggcct cgacgctcgc ctcttcaagc aactcgacgg cgcggcccgg cgagtctttc
    20101 gtcagcaggc gcgcggcctc cgtgagcgcc cacgcgcgct gcacggtcgt cagctcgtcg
    20161 gcgtgtgcga gccgcacggc gtcctcggcg cgcttgttgc ggatggctat ctccagaccg
    20221 cgataagcga gcgcgccgag gagctgtttg cgcgtgtcga tgtcttcgat cttggcggcg
    20281 tactcgcgcg cgcgtgtgag gtcgtcttcg gcgaagccca tggcgatgcg gaagtagatt
    20341 ctgtcgcgct cgcccggcgt cttggcgttc tcgatgcggc cgagcatgtt ctgaagctcg
    20401 tcttcgcgcg agggtttggc gacgaggccc tccgtgaaga ggtcgttgtc ggggcggcgg
    20461 acgcgttcgg gagtctcctg caagagcgcc gcctgccggg tgcgaacctg cgccgcgatg
    20521 tcgggcatat actgatcgaa gagcggcgcg aggcgcgtgg cgacgacgta agcgccggtg
    20581 cggcccgcgg aggtggggtc ttgctcgacg ggtggcagcg ggcgcgtgag tatttgcgcg
    20641 gcggcgcgca ggaaggcttc gcgcaggcgc gggtcgaggt cggcgggtgg ggcggcgttg
    20701 ccgcgccact ggcgcgtgtg gctgttgccg tcgggccgga cggtgatgta gaggaacggc
    20761 gtgaagacgt aagacgagag cagcgagacg gagtttgcgt ccgagttgga gtcggcgacg
    20821 gcgcgttgca gcagcgacat gaaacgctgg tcggcggctt tcgcgtcgcg ctcccgcagg
    20881 aggttgagga tgttcatgcc gaaggtgttg acggggtaga gcgccgggtc ggcgaactgc
    20941 atggcgcgat ccacctcacc atcctgaagg agctggctgg cgaggttgag gcgctgcgtc
    21001 atcgacgacg gcgggtcgtc ggggttgatg cgctgcgagg gcgcggacgt ggagcctgct
    21061 gctgtcggcg cagcgcccgc cggcgcgtcc tgtttggacg actcgtcgag gcgcttgagg
    21121 aattcgtcgc ccatcgcgct gtcgcgccgc gcggcctgac gcagcacctc gcggcgcacg
    21181 ctcccggcgt cgcgcgagtt ggctacgccg cggcgtccgg cctcgcgcgc acgctcctcg
    21241 cccgaatcgc ggtcggccgt ctcggccgcc tcccacgcgc gccggaacag cgcgcgcgac
    21301 ttcgccgcgt cagtctccca caacacgtcg gcggcgcgcg cctgcacttt ggcgcgcagc
    21361 gccatgtcgc cgaagttgcg cgcctcgtcg gcgagcgcga cgacgagcgc gacggcgctc
    21421 gcgcggcggg cctcggcgac ggggtcggtc tcggcggccg gggcgggctt gggcttgttg
    21481 cgttgattgc cggcggcggg ccgttgcgcc aggagaggcg cgggcgagag tgtcaggcag
    21541 agcgcgagga cgcgcagggt gtgtgcacga cggcggtggg acatttgtgt ttcctcggcg
    21601 ggcgcgccgg gcgtgccccc gctgaagggc ttcgttgcgc gccgcaagtt agttagtgac
    21661 tttaaggacg gaaagctctc gactttttaa ggcgtggccg tagccaccgc gcgcaagacg
    21721 agatgcaggg gtaaacgccc gtggcaggga ggcagttcca gaaagttatg agaggaagtt
    21781 gtcagtcgtc agtcgtttgt gtcgggcaac tcgccgctca cattgaaaga ctcaagccga
    21841 gctacggaaa caactgacga ctgacgactg acgactttac acggcgacga cttcgatctc
    21901 gggatcgact tcctgacgga tcatgccctc gatggccacc agcgtccccg acagcgagct
    21961 ggggcaactg ccgcacgcgc cctgatagcg aatggtcagg gtgttctctt taaggctcag
    22021 gacttcgagc cagccgccgt cgcccgcgag cgccggacgg atgcgctcgt cgagaatctc
    22081 gttgatgcgt agcaagcgtg ggtcgtcgct catcaaggcc ccgagcgccc cgccgacgcc
    22141 ggccgccgcg gcggctgccg ccaccggcgc gcccgccgca gccgacggcg cggcccgaat
    22201 cggcacggcg agccgcggca gaaggtcgtc ccagtcggcg tcctccttct tctcgaccgt
    22261 gatcatgtcg cccatgtaga agacggaggc cacttcgccc acctcgaaga gcgcgcgggc
    22321 gagtgcgtcg gcgcccgcct cctcgatgct gtgaaaagag cgcgccacgc tgctcgacac
    22381 cggctccttc agaatgaact tgacggcgtt cgggttcggc gtctcctcga tgttgtcgat
    22441 ctttggcatt aacttttctc cagtatgatg gcttcgatta tcccgccggc cggcaaccct
    22501 gcgcgcccgg tttctgcgtc atcaaaacgt gctctgatta tactccctcc ggcgcggcgc
    22561 ggcacccgcc ggggccgttc cggcccgcca aaaacggccc tcaaacgccc cctcggggcc
    22621 gtgtaaaaaa tccccgctcc accggtgaaa tttaacttgt cgaaaaatcg aagcgggcgt
    22681 atagtccatc cctcacttgt ccccctttcg caggtgagac aagctgagac tagaggcaga
    22741 cggtagcgag ggccgtctgc ctcttgtttt tttagaggtc agaggttaga ggttggaggt
    22801 cagtaaaaag cacttgcctt tactgacctc caacctctaa cctctgacct cttatgaata
    22861 ttggcatcac cgtctacccg acctacggcg ggagcggcat cgtcggctcg gagctgggca
    22921 aggaactggc ggcgcgcggc cacaccgtgc atttcatcgc gtcggcgctc cccacccgtc
    22981 tcaccgagct gaacgagcgg gtgcgcttcc acgaggtgga gatgatgtcg tacccgctct
    23041 tcgagcacca gccctacacg ctggcgctgg cgacgaagat gtcgaacgtg gccgaatcgg
    23101 aagacctcga cctcctccac gtccactacg ccatccccca ctcgatcagc gccatcctcg
    23161 cgcgcgaatc cctcaagccg caccgcgcgc ttcccgtcgt cacgaccctt cacggcacgg
    23221 acattacgct cgtcggcgcc gaccgctcgt atttgcccat cacacgctat ggcatcgcgc
    23281 agtccgacgg cgtgacggcc atctcgcatt accttgcgcg cgccacggcc gagactttcc
    23341 agttcgacca catcgccgtc atccccaact tcatctgcgg ctccgactac cgccgccgcc
    23401 ccgtgccgga gttgcgcgcg cagctcgcgc cgaacaatga gccgctgctc gtccacgtct
    23461 cgaacttccg gcccgtcaag cgccccgtgg actgcgtcga gattctcgcg cgcgtgcgcg
    23521 agcgcggcgt ggacgcacgg ctcgcgatgg tcggcgacgg cagcgagcgc ccgctgtgcg
    23581 agtatcgcgc gcgctgtctc ggcgtggaag agttctgcac cttcgtcggc aagcaaccgc
    23641 gtatcgtcga ttacctgtcg gcggccgacg tgctgctgct gccttcggag caggagtcgt
    23701 ttggtctggc cgcgctcgaa gcgatggcgt gcgaggtgcc ggtggtggcc tcgcgcgtcg
    23761 gcggcctccc cgaggtggtg acggacggcg agaccggctg tctggccgag gtgggcgacg
    23821 tggagcggat ggcggacgac gcggcgcgtc tgcttcgaga cgacgcggcg cgtcgggaga
    23881 tgggcacgcg agcgcgcgcg tcggcgcttg agcgttacag caccgacctc gtcatcccgc
    23941 agtacataga attttatgag cgcgtcatcg ccgcggcgaa agaaggccgc accgtctgaa
    24001 gggccggcgg cggcgcccga gtcggcgcgc gcgtgggagc gggccgccgg ccgtcacgcg
    24061 gcggaggaca gccgccccac gcgtccaacg aggccgggcg cgggaggtgt tcgcgcacgc
    24121 gcgatccgca ccgccgcgcc ggcgcacgac agccgccccg ccgccccggc gcacgacgtg
    24181 cgcgcaccag agaagcccat cgcctacatc gtccagaacc tcgaacgctc ctacggcgtg
    24241 cccgtcaaca agtggggcgg ctgggacccg ctcgacatgc tcgtcgccac catcctctcg
    24301 caggcgacga gcgacatcaa ctccgagcgc acctacgacg ccctcaagcg ccgcttcccc
    24361 aattgggacg ccgtcctgcg cgcacgccaa tcgacactcg ccgacaccat ccggcagggc
    24421 ggcctcgcca accagaaggc gcgcgtcatc cgcgacctgc tccgacaggt caaagaacgc
    24481 accggcgcgc tcaagctcga tttccttaat gacctgcccg tcgaagaggc cgcacagttt
    24541 ctctcgtcct tccgcggcgt cggccccaag accatcgcct gcacgctcct cttcgcctgt
    24601 cagcgcgaca tcttcccgct cgacacgcac atcttccgcg tcctccgacg cgtcggcctc
    24661 gtccccgaca ggtgctccga cgcgcgcgcc cacgagttga tgaacggcct cgtgcccaag
    24721 ggtaagttct actccttcca cgtcaacctc atccgccacg gccgccggct ctgccgcccg
    24781 cgcgacccac tgtgcgcgga gtgccctatt gtcgagtact gcgactacgg gcagacggtg
    24841 atataaaggg gacgacggac gacccaacac taccggcgtc gcgcacgaac tagattgggt
    24901 cgctgctccg aagagctgga gcgaatttta attgagcatt gggaacagag actcgccgcg
    24961 gaggcgcagg gaacgcaaga gttcacgccg cggagttgct tcaagcctgc ggcgcttcct
    25021 ccgcgccctg cggcgcctgc ggcggtgaat cgatttccca ttcactcaag tgaacagcgc
    25081 tctaaaaagc tcagaaccgt cccacgtccc cttgtttgaa aggattgaga aaatgaactg
    25141 catgaactgc ggcggctcgc tgacggcggg cgtgaagttc tgtccgcgct gcggcacgcc
    25201 caccccacag cccgacccct acgggcagca gcagcaatat gtcccgccgg gcgggcagat
    25261 gtactccacc tttgataccg gcgcgcagca gcagcagcag tacgcgcagc cgcgtcgcaa
    25321 gtcgcgcgcg ggcaagtttc tgctcatctt tctcggcgtc atcctcgtgc tcggcgccgg
    25381 cgcgatggcc gccgcgtatt tcggctaccg ctacatcgaa ggcacgttca agaactccga
    25441 gccgtacctg ctggccgtgc aggaactgag gcgcaacccc gtcgccgcgg aacacttcgg
    25501 ggagattaac gacacgggct ttccgctcgg caacttcaac gagagcgtgg gcggcacggg
    25561 ccacgcggag ttctccatgt ccgtcgaggg ggagaagggg agcgggaact acatggtcac
    25621 gatgtcgcgg acggacggcg tgtggagcgt caagaactcg gtcatcagga cgccggaggg
    25681 caagctcctc aacctctcgg gcaaacccag catcatcggc agcggcgacg tgggcagccc
    25741 cgaccccggc gcggcgacga acaactcgcg cgggcgggcg aacgccgccg cgggcgcgcc
    25801 ggcgatcagc ggcggcgtcc tcaacggcaa ggcagtctca aaacccgcgc ccccctaccc
    25861 ggccatcgcg aaggcggtgc gggcgtcggg gacggtgacg gtgcaggtga cggtagacga
    25921 agaggggcgc gtccgcgagg cgcgcgccgt gagcggacac ccgctgctgc gtgcggcagc
    25981 ggaagcggcg gcgcgcgagg cgcgtctgac cccgacgcaa ctctccggca agcccgtgaa
    26041 ggtgacgggc gtgctcacct acgacttcaa gcctgaatag gtcgcgcgac gggtgcaaac
    26101 ggccggggtg actgtcaggt ggcttacctc gtggacggca acaacgtgat ggggcaacga
    26161 gtcggctggc accgtgaccg gccgggcgcg cgccgccgcc tgctgggaga cctcgcgcgg
    26221 ttcgcgcgcc gtgaggaggc gagcctgacg gtggtcttcg acggcgcgcc cgacgaacac
    26281 ttccccgacg gctctgactt catgggcgtc cgcgtcttct acgccgagcg cggccgcacg
    26341 gccgacgacc gcatcaagtc gatggttgac gccgcgcgcg agcgccgcac gctcctcgtc
    26401 gtcacctccg accgccaact cgccgactac gtgcgccgct cgggcgtccg cgtccagcgt
    26461 tccggcgagt tccgcaagcg tctggacgcg acgcagaggc gcgaggacgc gcccgagaag
    26521 gccgacgagc cgcccccttc acacgacgaa ctgctgcgcg agatgcgcta cttcggcgtg
    26581 gggccggaag acgaagaaca gtgagcagta agcagtgagc agtgagcagt cagcagtcga
    26641 tagaacgtac tacctgcctt gactgctcac tgcttactgc tcactgctta ctgctttttc
    26701 gatgagcttt aaatcgaaga tgagcttgcg gccggcgagc gggtggttgc cgtcgatggt
    26761 gacgtgcgtg tcggtgactt cggtgacgac gacttcgagg tggttgccct cggggagcgg
    26821 caggttgagg ttcatgccga cctgcggctc gacgggaaac tccgcgttct cgcgctcgac
    26881 cgtggcgacg agctgctcgc ggcgcgggcc gtaggcgtcg tcaggcgcga tctcgacggt
    26941 gcgctcgccg ccgacctcca tgccgcgcac cgcttcctcg aagccgggga tgacctcgcc
    27001 cgacccgaga gtgaactcca gcggctcgcc gtcctccgac gtgtcgaaga cctcacccgt
    27061 ttcgagccgc cccgtgtagt ggacgcgcac ggtatcgccg tttttcaccg cctctggcat
    27121 aatctcggtt cacctcttcc gccagttttt cgacgcgcgc aagcatcgca catccggcga
    27181 ggcgaacaca acgcgcgagg gctggcgggg tgacccacgg ggccggacat gtctgcaact
    27241 cttccgggcg aagaaatatc gaacggaggc tttcgacgca tgaagaatct aacctcgccg
    27301 ctcgtcgccg ccctcctcgc gctccagccg ctcgtcgccc ccgtcgccct cccccacgcg
    27361 gcgtcgccgg cgaccgaaat cgcccgtgag actctgagcg cgcagacgag cgcgccgctg
    27421 ctgcccacgc ctacgcccgc cgaagcgggc gactccgatg tcgtccgcat cacgaccaac
    27481 ctcgtgcaga tcgacgccgt cgtcacggac aaacagggcc gcttagtcta cgacctcggc
    27541 cccgaagact ttgaaatcct cgaagacggt gagccgcgcg agatcaccaa tttctccttc
    27601 gtctccgccg gcgcggccgc cgcgggcgag cgcaccggct cggcctcgca gttcgtcgag
    27661 gtgcccaacc tcggtaagga gcgcctcgcc ctctccggca tggttctcat gggcgtcagt
    27721 agtagcagcg gcagcgccgc caacgacgcc gcgccgggcg cggcggatgc gtcgccgcaa
    27781 cagcagtcgc cggccgcgga gttcgacccg gacgccgtgc cggccgtgcg tcgcttccga
    27841 cagggcgcga gcatcgagta ctacctgcac gtctacaacg cgaagctcga cccggcgacc
    27901 ggcaagcccg tcttccagta ccacgcgcga ctcttccgcg acgctcagca ggtcttcgcc
    27961 ggcaaccccg tcaacttcaa tccgggccag cagacagaca tgaagcgcct gctcgtcggg
    28021 ggccgcctgc gcctcggcgc ggcgctcccg cccggcgaat acgtccttca ggtcgtcgtc
    28081 aacgacacgc tcgccaaaga gaaacaccgc atggcgacgc agtggatcga cttcgaaatc
    28141 gtcaaatagt tcaaagttca aagttcaaag ttcaaagcgc gtcggggccg ctgttgactt
    28201 tgaactttga actttgaact ttgaactacc ctatgtctct cctcttcgcc gccaccctct
    28261 tcctgagcgc ggcgctgctg ttctgggttc agccgctcgt ggcgaagatg ctgctgccgc
    28321 tcctcggcgg gacgccggcc gtctggaaca cctgcctgct cttcttccag ttcgtgctcc
    28381 tcgccggcta tgcctacgcc ctcgccctca cccgctggct ctccacacgc gcacaagtct
    28441 tcctccatgt tgcgctgctc atcgtggcgg ctgtgagttt gcctgtgggc ctgccggggt
    28501 cggccgtcga aggcgtgccc gaagggggcg agccggtgtg gtggctgctc gtgacgcttg
    28561 cggcgagcgt ggggctgccg ttcttcgctt tgtcggcgac ggcgccgctg ctgcaaaggt
    28621 ggttctcgaa gacacgcgac ccgcgcgccc gcgaccccta cttcctctac gcggcaagta
    28681 acgccggcag cctcgtcgcg ctcgtcggct tccccctcgt cgccgagccg aacctgcgcc
    28741 tcgccgggca gggccgcgcg tgggccgccg gctacgccgc gctcgtcctg ctcctcgccg
    28801 cctgcgcgtt cgcgttattg cgctcaggcc ccgcggaggt gaacgcgacg gccgcgaagc
    28861 cgcgcgacga agacgacgct tcttcggacg acgccgacaa cgacgcggat accgaaacgc
    28921 tgacaaagct ccgacgcctg cgctgggcgt ttctcgcctt cgtgccttcg agtctcgtgc
    28981 tcggtgtgac gacttacatc acgacggacg tggcggccgt gccgctgttg tgggtcgttc
    29041 cgctcgcgct ctatctgctc acgttcgtca tcgccttctc gcgcggacgg ctcctcgacg
    29101 ggcggctcgc cgaccggctc ctgcccggcg cggccatcct cctgacggtc gtctatctct
    29161 cgggcgcgac cgagccggcg tggttcctcg tcttagtcca tctcgtcttt ctctacatcg
    29221 ccgcgctcgc ctgccaccga cgcctcgccg acgaccgccc cgccccgcgc catctcgccg
    29281 agttcttcct gtgggtcgcc gtgggcggcg cgctgggcgg tctcttcaac gcgctgctcg
    29341 cgcctctgct cttcgacacc atcgtcgagt atccgctcgc cgtcgtctta gcctgcctcg
    29401 cgcgcgtccc ctttggcgcg cggaagagcc gcacgacgcc gggcgcgcaa cgcggcgacg
    29461 gcggcggcgc ggcgctcgac gactcaaacg aattgcgccg agaagaagac gacgcgcgga
    29521 gcagggaaga gagacgcgcg cgcgtgctcg acgtgttgtt gccggcggcg ttcggcgtcg
    29581 cggtcgccgt gctcacgggg gtcgtgacga tgttggagat ggggagcgtc gagcgcctcg
    29641 ccatctcgct cggcctgccg ctgctcatca tcaaccacgt cttccagcgc cgccccgtgc
    29701 gcttcgccct cgcgctcggc gccgtcatgc tctccacttt tctgctgacc gacggcggcg
    29761 ccaacacgct ccacgccgag aggaatttct acggcgtcgt gcgcgtcatg caggatgcga
    29821 gcaacgacac gctgcgtctg caccacggca gcaccatcca cgggcgacag ttcgccgacc
    29881 cggcgcgcgc ctgcgagccg ctttcttact atcaccccac ggggccgctc ggctcactct
    29941 tcgagacctt cgagacgcgc gccggagcca cggccgccac gtcgccgcac gtcgcggccg
    30001 tcggtctcgg cacaggcgcc gtcgtcgcct attcgcggcc cggccagagt tggacgttct
    30061 acgaaatcaa ccccgagatc gtccgcctcg cgcgcgacac gaacgcgttc acctacctgc
    30121 gcgactgcgc gcgcgcgccc gccgtggtcg tcctcggcga cgcgcgcttg cagatgcgcg
    30181 gcgcgcccga gtcgcactac ggcctcatcc tcctcgatgc cttcagctcc gatgccgtcc
    30241 ccgcgcacct gctgaccaga gaagccctcg acctctatct ctcgaagctc gcgccgggcg
    30301 gcctcatcgc cttccacgtc tcgaaccgcg gcctcgacct gcacgccgtc gtcggcggac
    30361 tcgcccgcga cgcgggactc gccgcgctcg tcttcgacga cccgagcttc gacaacgcga
    30421 gcggcaagga gccttccgag tgggtcgtcg tcgcccgccg ccccgaagac ctctcgacac
    30481 tcgctctcaa accgaactgg cagccgctga atgaaagtcc gcgccgccgc ttcgcggtct
    30541 ggcgcgacga cttctctgac atcatcagcg tctttaagtg gggcgggtaa aaagtaccgc
    30601 gagcaagtag cgtgaaacag taccgcgagc ggtagcgagc gggtgctgct gtgatgagag
    30661 acacgccacg cgcacgtgag acccgcgagg ctaccgctcg cggtactgtt gccagcgcgg
    30721 cgtcagggga gcttgccgcc ggaggccgaa gccccgccgg gcgtagtcac gagtttgtct
    30781 tgatggatgc gtctgaagag gcgggtcgcc agctcggggt cgtgagattg gagcgcgcgg
    30841 tgctgttcga gcgccgcttt tttgtcgccg gcttcggcgt aaagcagacc gaggcgatag
    30901 tgggctaagg cgaggtcagg cttgaggcgg agcgcctgtt tgaaagactc gacggcctcg
    30961 tcgcgccgac ccgaacggga gcaggcgtcg ccgcgccggt agtgcaactc cgccgacgcg
    31021 gggtcgagtt cgagtgcgcg tcggaaggac gcggcggcct cttcgtagcg gccggtgtcc
    31081 gcgtagacga cgccgaggtt gccgtgcccg gtggagaagt cgggcctgat gcggacggcc
    31141 tccttcagat gcgcgatggc ctcggcgacg cgcccgagct tgtgataagc ggcgcccaga
    31201 tcgctatgcg cctcgaccga cgccgcgtcg agacgcagca cctccttgag cgaggcgacc
    31261 gcctgctcgt gcatccccag agagatatac aacccgccga ggttgaagtg ggccggcgcg
    31321 gagtcgggcc ggagtcggac ggcgcggcgg caggcgtcga gcgcttcctc gtgcaggtgt
    31381 agcttcgacg ccgccacgca cagccccgcc tgcgcctccg acagattggg gtcgagcgcg
    31441 gcggctcgcc ggaacgaatc gacggcctcc gcgagacggc cccggttgtg ttgctcgacg
    31501 gcgctgaggt aagaggcgac ggcctgcgtc tgtgcgggcg acgggggagc ggtcttctgc
    31561 gccgtcgtgg tctgtgcggc caacgcgacg aacaggaagg cggccggacg aagggacggc
    31621 gcgcgcatgt gactctccct cttcattgag accgaaaacc ggggcggcga ggcgtgcggc
    31681 ttaaatctta tctttgaaac gtctgtcctg atttatgggc ggcactctac tgccgtgcgg
    31741 gagggaagtc aacggtttga aaatgtacgg gcgctgaggg cacagtaccg cgagcagtag
    31801 cgagcgggtc tcgcgtgcgc ggtgcgggct tttcattgta ggcacacccg ctcgctaccg
    31861 ctcgcggtac tgttccacct aactccaggc tggcacatat cgtgcaatca taaaaaataa
    31921 gaaagcgcga ataagttttc tcgcccgcgc cgcacgtcaa cacccgcacc gccagactaa
    31981 atctcacaca cgtaacacct tgccaactca cgcccgcact cggcacagac cttgccatgt
    32041 tgagagcgcg cgcggccgtc ccttccggct ccgaaccctt tgccgaaagg ttgtcgtccg
    32101 gccgcgcgct cagtacccgg aagagcctac atgaaccaag ggcagtaaaa gaccgcgccg
    32161 ctttaacacg aagggcgcgc aagatgtgag gagatgttta tgtcaatcaa aaaatggatg
    32221 ctcacggccc tcttgctgtg cgcgtgcgga gcatcggcgc tggcgcagac cgagaccgga
    32281 cagattacgg ggaaggtctt agacccgaac ggcgcactgg tcgccggagc ggccgcgacc
    32341 gtcaagtcag tcgagacggg ccgcgagcgc gccgccacca ctaacgagga gggcgtctac
    32401 atcgtcgcca atctccagag cggcctctac gacgtgaccg tcaacgccac ggggttcgcc
    32461 ccgagcacgc agcgcgtgca ggtgaccgtc ggctcgcagg tctccgtgga gacggcgctc
    32521 accatctcag ccatccccgg cgagaccgtg gaagttgtcg ccggcggcgg cgtggaagtg
    32581 aacacgcaga cgcaggaact ttccaccgtc gtctccggcg cgcagatcag ggagctgccg
    32641 accgtcacgc gcaaccccta taacctcgtg caactctccg gcaacgccgc gaccgatgac
    32701 cccaccacct cacagaacga caccgccggc acggccacct accgcggcgc cggcgcatcc
    32761 ttgaacggcc tgcgcgcggc ctcgaccaac atcctcttag acggcgctga caacaacaat
    32821 tcctacacgg cgcaggtcgg ccagtcgatt ccgctcgacg ccgtgcagga gttccgcgtc
    32881 atctccagca acttcacggc cgagtacgga cgcgcttcgg gcggcatcgt caacgtggcg
    32941 acgcgcgccg gctcgaacca gttccacggc acggcctacg ccttcaatcg cctctcgcgt
    33001 ctcgcctcga acgggttcgc caacaacgcc ctcggcgtcg agcgcggcgt cttcacgcgc
    33061 aaccagttcg gctactcggc cggcggcccc gtcatcaaga acaagttgtt cttcttcaac
    33121 tcgacggagt ggacgcgcgt gcgctcgacg ggcgagctga cctctttcgt cccgacctcg
    33181 gaactcatcg ggctgtcggc cgcgaacacg cgcgacttct tcagcgccta caacgtcagc
    33241 gcccggccga cgggccgcac cgtctcggcc gccgacctcg gcggcatcag cggcatcccc
    33301 ggcgccaccc cggccttccg cgaagtggcc ttcaccgtcc cgaccgacct cggcggcggc
    33361 ttcccgcaaa acgatgtctc gaccgttacc cgcatcgact ggaacatcaa cgaccgcacg
    33421 cagctctatg gccgctacgc cctacaggat cagattctct tcgagggcgc cggcgcgttc
    33481 agtccctatg agggcttcaa cacgccgacc tcgcagttca accagaacgc gctcctgtct
    33541 ttgacgcgca ccttctcgca aaacctcgtc tccacgtcga aggttgcctt caaccgcgtc
    33601 aactcgcaga acccgctcgg cgcgcagccc gccggcccga ccctctacgt gtttgagaac
    33661 gtggcggcag ccattccggg cacggccacg gccatcgcgt tccccggcta tctgccgttc
    33721 aatcccggca gcgccatccc ggccggcggc ccctctaacc agttgcagtt ctatcaggac
    33781 ttcaactacc agcgcggcaa ccaccagttc cgcttcggcg gcaactacat caacatccag
    33841 cagaaccgca ccttcggcgc cttccagaac tccgtgctca cgctcggcaa caccttgaat
    33901 caggcgctca acaacttggt cgccggccag ctcctcaagt ttcagggcgc ggtcgatccg
    33961 cagggcaact tccccggcga cttcgtcacc acgccgctcg ggccgcccag cttcagccgc
    34021 agcaacgtct ataacgagtg ggccgcttac ctcaacgact cgttccgcgt gctcccgaac
    34081 gtcaccttga acctcggcgt gcgctacgag tacttcggcg tgcagcacaa caagaatgcg
    34141 cggctcgact cgaacttcta cttcggcgag ggcgcgacca ttcaggagcg catccgcaac
    34201 ggcagcgtgc agatcgccga agacagcccc gtcggcggcc tctgggcgcc cgacaagaac
    34261 aacttcgcgc cgcgtctcgg catcgcctgg gacatcaccg gcgacggccg cacggccctg
    34321 cgcggaggct acggagtggc ctacgagcgc aacttcggca acgtcacctt caacgtcatt
    34381 cagaacccgc ccaactacgc cgtcgtggcc gtcacggccg gcgcggaagt ggactccatc
    34441 ccgctctccg gcaacaacgc cggcccgctc gccggcagcg gcaccacgct ccgcttgccg
    34501 acgacctcgc ttcggaacgt gcgccccgac atccgcaacg cctacgcgca cttctacagc
    34561 gcctcgttcg agcgtgagct gggccgcaac accgtggcgt cggtcgagta ctctggctct
    34621 tcggggcgca agctctactc gctggagaac tacaaccgcg tcggcgcggg gccggtcttc
    34681 ctcggcagca acgccgtgac gccgaccggc ggcgcctcgt cgcgtctgaa cggccagtac
    34741 tcgaacgcca acacgcgcgg caacggcggc tactcgaact acaacgccct gattctgggc
    34801 ttcgagagca acagcttccg ctcgtcgggt ctccagttca cggcccgcta cacgctgtcg
    34861 tcggcgaagg acaacctgag ttcgaccttc tccgactcga acgccaactt caacctcggc
    34921 ctgctcgacc cgttcgaccc ctcactcgat tacggctacg ccgactacga cgcgcgccat
    34981 cgcttcgtcg ccagcttcaa ctacgaagtg ccctacttcg accggctcga aagcaagttc
    35041 gcgcgccacg tcctcggcgg cttctcgctg acgggcatct tcaacgcgcg caccggcaac
    35101 ccgttcaccc tcgtcgattg cacgagcggc gcgggcggcc tctgtcaccg ctacattccg
    35161 ggcgggagcg ccggcatctc cggctcggac aatccgcgct cggtcggcgc caacgccttc
    35221 gcctacatcg acgtgcccgc cggcgtcgtc tacaccaacc cggtcgcggg caacggcgcg
    35281 ttgccgccat tccccgcgga gatgagcgcc cgcaacgcct tccgcggccc cggctactgg
    35341 aacttcgacg ccggcctcta caagcgcatc aggttctcgg agaagtacgc cttgcagctt
    35401 cgtctcgaag ccttcaacgt cttcaaccac tcgaacctgt tcatcgcgcc gggcggcgcc
    35461 ctcgacctca gccagtttca caacttcgac gccgacggca acctcattaa cacccagccc
    35521 ggctacgcca ccgcccagcg cggcgtcttc ccgagcggca acctcgagcg gcgcaacgtg
    35581 cagctcgcgg tcaagttcat cttctaactt cg
//
LOCUS       DASVES010000224        13307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_674907, whole genome shotgun sequence.
ACCESSION   DASVES010000224 DASVES010000000
VERSION     DASVES010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13307
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674907"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..701)
                     /locus_tag="VF240_09080"
     CDS             complement(387..701)
                     /locus_tag="VF240_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175398.1"
                     /translation="MELAGTWNLEVSTPFGKHPATLVLEREESGNFGGHIDSRLGNAT
                     LAGVSANEAGGFDAVVTLELQGRAYEARVNATVEDGRMDGTIKVNIPLAPPARFTGRR
                     GE"
     gene            complement(720..2174)
                     /locus_tag="VF240_09085"
     CDS             complement(720..2174)
                     /locus_tag="VF240_09085"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7175399.1"
                     /translation="METHTETFRREPPPPLAHRTLNLPVPFETTLPNGLTLVVVEQDR
                     LPLVNFRLALPTGDAHDPPAIPGLTDLLTHMLNEGTAEHTSRQLAEEVARLGATLTAG
                     ASSDYTTVAASALSAYGDEMLALLAEVALRPNFPENELELARQNSHQNLIAQRAQASF
                     LATERTARVMFGEHPYHVVSPTHESIDATTRDALAAFHRARFVPRGAVLAVVGDVRRE
                     AIVARVEELFGGWEGTAAEESRFPAPPERRERVAYVVDRPGSAQSNIIIANLAITRTH
                     PDYFPLLVMHTILGGTASARLFMNLREEKGYTYGAYSSLDARRHAGTFRATAEVRTPV
                     TAASLKEFFYELGRIRDEEVAEEELANAKSYLTGVFPIRLETQEGVIDQLVQMKMHGL
                     PDDYLHRYAERVRAVTREDVRRVAREFITPERAAVVIVGDAGAVAEQIRPFVERMEFY
                     DGEGQPMETPAGATTEADGETAPPDTHEEAAKAE"
     gene            complement(2243..3718)
                     /locus_tag="VF240_09090"
     CDS             complement(2243..3718)
                     /locus_tag="VF240_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7175400.1"
                     /translation="MSATLTLPRVGVEQFALANGLRVVLSEDHSVPVVSVAVYYDVGS
                     RNEREGRTGFAHLFEHMMFQGSENVPKAGHFQYIFNNGGTMNGTTSTERTNYFETLPA
                     SQLPLALWLESDRMRSLKVTQENLDNQRDAVKEEKRLNYDNRPYSNAFLHLNELIFRN
                     PANAHSTIGSMEDLDAATIDDIREFFRIYYAPNNAVITIVGDFDPREARALVEKYFST
                     IPAQTRPAPAEVEEPEEVAVRRETYRDPFAQLPGLMLGWKTPARRTTDGYALSLAADL
                     LLEGDSSRLYQRLVKDEESVVGIQGGIGERRGPSSLYVFAIPKPGHSTEAIKATIDEE
                     IRRLAAEGPAPQEMEKLRNNILNDFTRGCQSTLYRAQRLSEYTLYDGDPELINGEVER
                     YLAVTPDEIKRAAARYLMTENYSLLEVVTARAADGAEAAEAVGAPPGEPDEPGSPPPQ
                     VPQPPPSDAPSTASASLPTEPSRPASPGQPASPSEPPPPEV"
     gene            complement(3806..4261)
                     /locus_tag="VF240_09095"
     CDS             complement(3806..4261)
                     /locus_tag="VF240_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175401.1"
                     /translation="MSNPYDPKTSNPLDPNRPDNFSEPIDDLMITDDAEGGEAGDRDR
                     ISSSMRDTLHRIPESLSSIGRDISRTIERAISAKDEYVVAVKLSPEAQDKIDQLVAAG
                     VFRSRAEAAAFLIGEGINAQSELFGRVGQKLSEIERLRAELRGMISKTE"
     gene            4629..6416
                     /locus_tag="VF240_09100"
     CDS             4629..6416
                     /locus_tag="VF240_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7175402.1"
                     /translation="MKRIITSAFALLALLLPSFAIAQPQTPARRGDQGAASTTPATPA
                     GRNRRERAPTRPDAPAGAGAVSLEGIEGDLSEALSVIQDNYVEGVKLDYNNVFKSSII
                     GMLRSLDPHSNFYDAKEFEEQRADWRSEYYGIGATIGDRKIGERTDTYILATFDNTPA
                     HKAGLRFGDRIVDVDGQSMQGKNSGEVRDRLRGPRSSSVKVTVERAASGNKETVEITR
                     DAVSQPTVPDFYMIRPGVGYVDMQRGFNYTTADEFVEALEKLRAKGMTSLVLDLRNNG
                     GGILDQAVKVAEQFLPEGQRILTQKGRSRRSGGERPYDSSNPNPDTSPVVVLVNRGTA
                     SASEIVAGALQDHDRGLIIGETTFGKGLVQSIMPLDYGTALTLTTSKYYTPSGRLLQR
                     DYSQTSFYDYIYRGGIGSQDDDENRKGEEQKQPEKKGPESFTDTGRVIYGGGGIAPDE
                     SVKPRLISVPQQRLIDPAFAFAREVVNGRVSGFEQFKVARMPDFEHNLRAEDFSVDDK
                     LFKAFKEYVATKGQGYKLTDAQLDKHREFVRRQLRYDLATAAYGTVKASQVLVSDDPQ
                     VTKAIELLPRARELAANASRGRGQQKSYE"
     gene            complement(6497..7153)
                     /locus_tag="VF240_09105"
     CDS             complement(6497..7153)
                     /locus_tag="VF240_09105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX7175403.1"
                     /translation="MPAKNEDLHGSAPDKSEVALLLIDVINDLDFEQGRQLLEHARPM
                     AERIAALKRRAREAGFPVVYVNDNFGRWQSDFNKILAHCIDDDSTGRPVAEMLRPEED
                     DYFVLKPKHSGFYSTTLDTLLDYLQAKTLILTGMATDVCVLFTANDAYMRDFHLIVPA
                     DCVASHDPRESEHALRVMADLLKADTTLSERLDLEALKRRAVEESQPRRPPHQAAARS
                     "
     gene            complement(7327..7767)
                     /locus_tag="VF240_09110"
     CDS             complement(7327..7767)
                     /locus_tag="VF240_09110"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX7175404.1"
                     /translation="MAKINYLAVGVAGVLYWILGALWYGLFSERFIALMRWTPEDLAR
                     IQAQGSAKELVLAFVTSMLTAYVLAHFVRYAGARTGLDGALTGFWAFVGFVLTTNLAT
                     VIFEGRPAGLYLISMGYNLVALLLMGVLLAVWRRQEARVPAYQT"
     gene            complement(7945..8445)
                     /locus_tag="VF240_09115"
     CDS             complement(7945..8445)
                     /locus_tag="VF240_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7175405.1"
                     /translation="MKNRLSLGLLVLLFFAAGYGVTASTGSAAEPESKDEIARAIIAR
                     EKASAEAWQRKDKAFFADYMAADSTYFGPMSPYLETDPKENFLPKFEQFAERFKMVDW
                     QMYTPRVQTYGDTAILTYNVGATVDIGGRPMQYTGKVTQVYVRQGGVWRVVHGHESIN
                     PGPQQQ"
     gene            complement(8649..9053)
                     /locus_tag="VF240_09120"
     CDS             complement(8649..9053)
                     /locus_tag="VF240_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7175406.1"
                     /translation="MADQHHEMPAHGTFCWTELSTKDAEAAKKFYTELLGWEIKTSDG
                     AGMVYNEIVVGGKHVGGIYQAGPECGGEMPTRWSSYVAVDDVDASAKRVEELGGKVSV
                     PPMDIPNTGRFCVITDPTGANISLITLKGASS"
     gene            complement(9234..9758)
                     /locus_tag="VF240_09125"
     CDS             complement(9234..9758)
                     /locus_tag="VF240_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175407.1"
                     /translation="MLTEEIIQEHAPISLELEYADEPRAGVGTAERASEREGRIELPF
                     SKKDEILDLYESGTTDVAEIVRKVHARPSYVAQVLQTAGHLSGYFDLYTTTGAEQNVY
                     TRFFRNVLSFKSMDAARESVEKIDRLYNYFERLGDRAGQHQAMVIALTGKNRARWSGK
                     LEESKIFSEWLVAH"
     gene            complement(9851..11146)
                     /locus_tag="VF240_09130"
     CDS             complement(9851..11146)
                     /locus_tag="VF240_09130"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HEX7175408.1"
                     /translation="MAAVLDKKAIMRDKRAQGIAAMGLVNREGDRFHVTTPSLRGKRA
                     AYEVWRDEAGHVRCSCLEFEELSAGEPTFRCEHILAVKHSLLNKSSEPVTKQAANRPL
                     AQAGAGISTVNVLAETPNTQDKNASEGRDEATEATVRTAASRQPRFVREQTPEPQDEV
                     EATKNDAPADAILLTGSLATHEDEKEHQEMSKRATANVQEPEVAQDFEGEFEHASSAS
                     VVPMAFTNTLRVLRQPVDAKLVKTREGWTDRNGNRHMVEYVEWHTVADILDRVAPTWS
                     HSVRNIVQIGDMVAVTCAITVDGVTREGVGTGTADNETGIKKAEHDALKRAAIKFGIA
                     RELYQRESEVIERDGAGPQPGDFPRDPLAKSMADLVTPKQLGMIRALAREAGVDAEEE
                     CQAQMRCRTEELSKRAASSFIDHLKGAQTEAAGGMRRAS"
     gene            complement(11175..11366)
                     /locus_tag="VF240_09135"
     CDS             complement(11175..11366)
                     /locus_tag="VF240_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175409.1"
                     /translation="MEHIWETFDGLFKTVYRQSRKVEELETRIASLERRLQERDGSGA
                     EKHSTERVMVTELPVERAA"
     gene            11581..12009
                     /locus_tag="VF240_09140"
     CDS             11581..12009
                     /locus_tag="VF240_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7175410.1"
                     /translation="MADNANNLVNTVELGRAIRRKREDAGLSLRDVADETGVSASTLS
                     RIENGTGKPDADNIARLTGWLNMPMERVMGNRAAAGDLEDKAVVYFPKEAMPDIVEAH
                     LRADRNLTPETAKALSELFRVAYQQFSHPGEDTRGRNKRK"
     gene            12130..12372
                     /locus_tag="VF240_09145"
     CDS             12130..12372
                     /locus_tag="VF240_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175411.1"
                     /translation="MSTRTLRAHFDGEQVCLDEPAQLEPGTRLIITVLPGRILDNGDE
                     DWSLLSLEGLAGAYGKDEPEYPTELIKELNPNYEGR"
     gene            12815..13294
                     /locus_tag="VF240_09150"
     CDS             12815..13294
                     /locus_tag="VF240_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7175412.1"
                     /translation="MSDKVKPIPEGYHSVTPYLCVSDAARAIEFYKEAFGATEVGRME
                     APGGRIGHAEIKIGDSHVMLADEFPEINFRSPQTLGGPTAQFMIYVEDVDARVEQAVA
                     AGAKLVRPVKDQFYGDRSGGVEDPFGHFWYISTRIENLTHEEVKKRSDAEITQHAGA"
BASE COUNT         2285 a         4133 c         4430 g         2459 t
ORIGIN      
        1 cgactccgcg gtgagtccga ctttccactt actcaactga acattgatat agaggtaggc
       61 tctggacact gataaacggt taaactgtac tgtgaagccg cgacaaagat tcgagcaacg
      121 cgccgtgtga gacgggcatc cgaaaggatt agcatagcaa gttgcccgac aggtcgcttt
      181 ggggaaagcg gccggggtca acggatgatt ctcctgtgat ttccgcccgg cgttgagcca
      241 cgccgggcgg tttttttgtg agcagttagc ggttagctat tagctgttag cccacggcag
      301 ccgccgaagg gcgtgccgcc gcttaaaact caagtccctt gttcgctctc tttaagctaa
      361 cagctaatcg ctaatcgcta acagcttcat tccccccgcc tcccggtgaa gcgggccggc
      421 ggggcgagcg ggatgttgac cttgatggtg ccgtccatcc ggccgtcttc gaccgtggcg
      481 ttgacgcgcg cctcgtaggc gcgcccttgc agttcgagtg tgacgacggc gtcgaagccc
      541 ccggcctcgt tggcgctgac gccggcgagt gtggcgttcc cgaggcggga gtcgatgtgc
      601 ccgccgaagt taccgctctc ttcgcgctcc aaaacgagcg tcgccgggtg cttgccgaag
      661 ggcgtcgaga cttccagatt ccatgtgcct gcgagttcca agtcagaatc ctcccttgct
      721 cattccgctt tcgcggcctc ttcgtgcgtg tccggcgggg ccgtttcccc gtcagcttcc
      781 gtcgtggcgc cggcgggcgt ttccatcggc tgcccctcgc cgtcgtagaa ctccatgcgc
      841 tcgacaaagg gcctgatctg ctcggcgacg gcgcccgcgt cgccgacgat gacgacggcc
      901 gcgcgctcgg gcgtgatgaa ctcgcgcgcg actctccgca cgtcctcgcg cgtgacggcg
      961 cggacgcgct ccgcgtacct gtgcaggtaa tcatcgggca ggccgtgcat cttcatctgc
     1021 acgagctggt cgatgacgcc ctcctgcgtc tccaggcgga tgggaaacac tccggtcaga
     1081 taagacttgg cgttggccaa ctcctcttcg gcgacctctt cgtcgcggat gcgtcccaat
     1141 tcgtagaaga actctttgag cgaggcggcc gtgacgggcg tgcgaacctc ggccgtggcg
     1201 cggaaggtgc cggcgtggcg gcgcgcgtcg aggctggagt aagcgccgta ggtgtagccc
     1261 ttctcctcgc gcaggttcat gaagaggcgg gcggaggccg tcccgccgag gatggtgtgc
     1321 atgacgagca gggggaagta gtcggggtgc gtgcgcgtga tggcgaggtt ggcgatgatg
     1381 atgttcgact gtgccgagcc ggggcggtca acgacgtatg cgacacgctc gcgccgctcg
     1441 ggcggggcgg ggaagcgcga ctcttcggcc gccgtgccct cccagccgcc gaacaactct
     1501 tcgacgcgcg cgacgattgc ctcgcgccgc acgtcgccga cgacggcgag gacggcccca
     1561 cgggggacga agcgcgcgcg gtggaaggcg gcgagcgcgt cgcgcgtggt cgcgtcgatt
     1621 gactcgtgcg tgggcgagac gacgtggtag ggatgctcgc cgaacatgac gcgcgccgtc
     1681 cgctcggtgg cgaggaagga ggcttgcgcg cgctgcgcga tgaggttctg gtgcgagttc
     1741 tgccgggcca gctcaagctc gttttcgggg aagttgggac gcagcgcgac ctcggcgagc
     1801 agggcgagca tttcgtcgcc gtaggccgag agcgcggagg cggcgaccgt ggtgtagtcg
     1861 gagctggcgc cggccgtgag cgtggcgccg aggcgggcca cctcttcggc gagctggcgg
     1921 ctcgtgtgct ccgcggtgcc ctcgttgagc atgtgcgtca ggaggtctgt gaggccggga
     1981 atcgcgggcg ggtcgtgcgc gtcgccggtg gggagcgcga ggcggaagtt gacgagcggc
     2041 aggcgatcct gctcgacgac gacgagcgtc aggccgttcg ggagcgtggt ctcgaacggc
     2101 acgggcagat tgagcgtgcg gtgggcgagc ggcggaggcg gctcgcgcct gaaagtctcc
     2161 gtgtgagttt ccattgaatg tatcttaaac gatgggggga tgggtgaatt gacgccggaa
     2221 gatcagttcc gacacgacgc gctcagacct ccggcggcgg cggctccgaa ggcgatgccg
     2281 gctgacccgg cgacgcgggc cgcgacggct ccgtgggtaa tgaagcagac gcggtcgaag
     2341 gggcgtccga agggggcggc tgcggaacct gaggcggcgg ggaacccggc tcatccggct
     2401 cacccggcgg cgcgcccacg gcttcggcgg cctccgcgcc atcggccgcg cgcgccgtga
     2461 cgacttcgag cagggagtag ttttccgtca tcaggtagcg ggcggcggcg cgcttgatct
     2521 cgtcgggcgt cacggcaagg taacgctcga cctccccgtt gatgagttcg gggtcgccgt
     2581 cgtagagggt gtactcggag agccgttgcg cgcggtagag tgtcgattgg cagccgcgcg
     2641 tgaagtcgtt gaggatgttg ttacgcagct tctccatctc ctgcggcgcg gggccttcag
     2701 cggcgagccg gcgaatctcc tcgtcgatgg tcgccttgat ggcctcggtc gagtggccgg
     2761 gcttggggat ggcgaagacg tagagcgagg aggggccgcg gcgctcgccg atgccgccct
     2821 gaatgccgac caccgactcc tcgtccttga cgagacgctg gtagaggcgc gagctgtcgc
     2881 cttcgagcag gaggtcggcg gcgaggctga gcgcgtagcc gtccgtggtg cggcgcgcgg
     2941 gcgtcttcca gccgagcatc agtccgggca actgcgcgaa cgggtcgcgg taggtctcac
     3001 gccggacggc gacctcctcc ggctcttcta cttcggccgg cgccggacgc gtctgcgcgg
     3061 gaatcgtcga gaagtacttc tcgacgaggg cgcgcgcctc tcgcgggtcg aagtcgccga
     3121 cgatggtgat gacggcgttg ttgggcgcgt agtagatgcg gaagaactcg cggatgtcgt
     3181 cgatggtggc ggcgtcgagg tcttccatcg agccgatggt cgaatgggcg ttggccgggt
     3241 tgcggaagat gagttcgttg aggtggagga aggcgttcga gtaggggcgg ttgtcgtagt
     3301 tgagccgctt ctcctccttg acggcgtcgc gctggttgtc gaggttctcc tgcgtcacct
     3361 tgagcgagcg catgcggtca ctctccagcc acagcgcgag cgggagctgg ctggcgggga
     3421 gcgtctcgaa gtagttggtg cgctcggtcg aggtcgtccc gttcatcgtg ccgccgttgt
     3481 tgaagatata ctggaagtgg ccggccttcg gcacgttctc cgagccttga aacatcatgt
     3541 gctcgaagag gtgggcgaac cccgtgcggc cctcgcgctc attgcgcgag ccgacatcat
     3601 aatagacggc caccgagacg accggcaccg agtggtcttc gctgagaact acccgcagac
     3661 cattcgcgag cgcgaactgc tcgaccccga ccctcggcag cgtgagtgtg gctgacaaaa
     3721 gaatctccta agaacagagg tcagaggtta gaggtcagag gttagtgaaa gacaagatgc
     3781 tctttactga cctctaacct ctgctttatt ccgtcttgct gatcatgccg cgcagctcgg
     3841 cgcgcaggcg ctcgatctcg gagagcttct ggccgacgcg gccgaagagc tcggactgcg
     3901 cgttgatgcc ctcgccgatg aggaaggcgg cggcctcggc ccgcgagcgg aagacgccgg
     3961 ccgcgacgag ctggtcgatc ttgtcctgcg cctcgggcga gagcttcacg gccacgacgt
     4021 attcgtcctt ggccgagatg gcccgctcga tggtgcggct gatgtcgcgg ccgatggagc
     4081 tgagcgactc ggggatgcgg tggagcgtgt cgcgcatcga ggaggagatg cggtcgcggt
     4141 cgccggcctc cccgccctcg gcgtcgtcgg taatcatcag gtcgtcaatc ggctcggaga
     4201 agttgtcggg ccggttcggg tcgagcgggt tgctcgtctt cggatcgtaa gggttgctca
     4261 tcactactcg gtgacctcct tcgcggacac agcgacgccg ggctttcgtg acgcccggct
     4321 ctactgccca acatttcgtg ctcgcgcttg tactgaattc aagaatcatt ctacgggcgc
     4381 gtcaggggcg agcgcaagtg gcgtcgttca ggcgattagg tgggcttcgt ccgggggatg
     4441 tgagcagccg cgcggccctt tcttgtaaaa attgtaaacc ccggcgaggc gcaaccttgc
     4501 cgggggttta ctcatccatt aaactacaca aacctgccgg cgccccgacg tttccgagca
     4561 ggttctaaat cgccttcggc aaggcgccac gatcaaaccg ggttacgtgc cacaagaggt
     4621 tttgccccgt gaagagaatc atcaccagcg ccttcgcact tctcgccctc ctgcttccgt
     4681 ctttcgccat cgcccagccg cagacgcccg cgcgccgcgg cgatcagggc gcggcctcga
     4741 ctacgccagc gacgcccgcc ggccgcaacc gacgcgagcg cgccccgacg cgccccgacg
     4801 cgccggccgg agcgggggcc gtctcgctcg aaggcatcga gggcgatctc tccgaggccc
     4861 tgagcgtcat tcaggacaac tacgtcgagg gcgtcaagct cgactacaac aacgtcttca
     4921 agtcgtcgat catcggcatg ctgcgctcgc tcgacccgca ctcgaacttc tacgacgcga
     4981 aagagtttga ggagcagcgc gccgactggc gctcggagta ctacggcatc ggcgcgacca
     5041 tcggcgaccg caagatcggc gagcgcacgg acacctacat cctcgccacc ttcgacaaca
     5101 cgccggccca caaggccggg ctgcgcttcg gcgaccgcat cgtcgatgtg gacggccagt
     5161 cgatgcaggg caagaattcc ggcgaggtgc gcgaccgcct gcgcggcccg cgcagctcca
     5221 gcgtcaaggt gacggtcgag cgcgccgcgt cgggcaacaa ggagacggtc gagatcacgc
     5281 gcgacgccgt cagccagccg accgtgcccg acttctacat gattcgcccc ggcgtcggct
     5341 acgtcgatat gcagcgcggc ttcaactaca cgacggccga cgagttcgtc gaggcgctgg
     5401 agaagctgcg ggccaagggc atgacctcgc tcgtcttaga ccttcgcaac aacggcggcg
     5461 gcatcctcga tcaggccgtg aaggtggccg agcaattcct gcccgagggg cagcgcatcc
     5521 tgacgcagaa agggcgcagc cggcgcagcg ggggcgagcg tccatacgat tcctccaacc
     5581 cgaaccccga cacctcgccg gtcgtcgtct tagtcaaccg cggcacggcc tcggcctccg
     5641 agatcgtcgc cggcgctttg caggatcatg accgcggcct catcattggc gagacgacct
     5701 tcggcaaggg cctcgttcag tcgatcatgc cgctcgacta cgggacggcc ttgacgctga
     5761 cgacctcgaa gtactacacg ccctccggcc ggctgctcca gcgcgactac tcgcagacga
     5821 gcttctacga ctacatctat cgcggcggca tcggcagcca ggacgacgac gagaaccgaa
     5881 agggcgagga gcagaagcag cccgagaaga aaggccccga gagtttcacc gacacggggc
     5941 gcgtgatcta cggcggcggc ggcatcgcgc cggacgagtc cgtcaagccg cgtctcatct
     6001 cggtgccgca gcagcgcctc atcgacccgg ccttcgcctt cgcccgcgag gtggtcaacg
     6061 gccgcgtctc cggcttcgag cagttcaagg ttgcgcgcat gcccgacttc gagcacaacc
     6121 tccgggccga agacttcagc gtcgatgaca agctcttcaa ggcgttcaag gaatacgtcg
     6181 ccacgaaggg gcagggctac aagctgacgg acgcgcagct cgacaagcat cgcgagttcg
     6241 tccggcggca actgcgctac gatctggcga cggccgccta cggcacggtc aaggcgtcgc
     6301 aggtcttagt ctcggacgac ccgcaggtga ccaaggccat cgaactcctg ccgcgcgcac
     6361 gcgaactcgc cgccaacgct tcccgcggac gcggccagca gaagtcttac gagtaagcaa
     6421 ccaggacacg agtcctgaac actcaagggg cgctcaaacc tgtgcgggtt tgagcgcccc
     6481 tcttgttttc gtagtctcaa ctccgcgctg ccgcctgatg cggcgggcgc cgaggctgcg
     6541 actcctccac ggcccggcgc ttgagggctt cgaggtcgag gcgttcagag agggtcgtgt
     6601 cggccttgag caaatctgcc atcacgcgca acgcgtgctc gctctcgcgc gggtcatggg
     6661 aggcgacgca gtcggcggga acgatgaggt ggaagtcgcg catgtaggcg tcgttggcgg
     6721 tgaagaggac gcacacgtcc gtcgccatcc ccgtgaggat gagcgttttg gcctgtaggt
     6781 agtcgagcag ggtgtcgagc gtcgtcgagt agaagccgga gtgcttgggc ttgaggacga
     6841 aatagtcgtc ctcttcgggc cggagcatct cggcgacggg gcggcccgtg gagtcgtcgt
     6901 cgatgcagtg ggcgagaatc ttgttgaagt cggactgcca gcggccgaag ttgtcgttga
     6961 cgtagacgac ggggaagccc gcctctcgtg cgcggcgctt gagggcggcg atgcgctcgg
     7021 ccatcggccg ggcgtgttcg aggagctgcc gcccttgctc gaaatcgagg tcgttaatga
     7081 catcgatgag gagcaaggcc acttctgact tgtcgggcgc gctgccgtgc aggtcttcgt
     7141 tcttcgcggg cataagcgtt cgagcttgac tggcctccga gtaatgattc gggaaaacgg
     7201 ggaagggaaa ttatgcagtc cattccccga aacgggcaag tgacgtgcag acaaaggcgc
     7261 cgccgcgcgg tgagggcgcg ggcggcggga gggagaccgt cgtctccctg cgggtgtgtg
     7321 cgcgcttcag gtctgatacg ccggaaccct cgcctcctgc ctgcgccaga cggcgaggag
     7381 cactcccatc aggaggaggg cgacgaggtt gtagcccatg ctgatgaggt agaggccggc
     7441 gggacggccc tcgaagatga cggtcgccag attggtcgtg aggacgaagc cgacgaacgc
     7501 ccagaagccg gtgagcgcgc cgtcgaggcc cgtgcgcgcg cccgcgtagc ggacgaaatg
     7561 cgcgagcacg taggccgtca gcatcgaggt cacgaaggcc agcaccaact ccttcgccga
     7621 accctgcgcc tggatgcgcg cgaggtcttc gggcgtccag cgcatgagcg cgatgaatcg
     7681 ctccgagaag aggccatacc agagcgcgcc gagaatccag tagaggacgc ccgcgacgcc
     7741 gaccgccaag tagttgatct tcgccattgc gttttctcct tcgagtgcgg aaagtgtttc
     7801 ggcaaacgcg cccgttctac ggccctgcgc tcgacgcgcc ccgcgcgcac gcccgtcagg
     7861 ggggaggggg cggcgtgcgc gttggggcgc gcgcgtttcg cgcgagcgaa gccggtgggc
     7921 cgctccgctt ggccgcgcac gatgctactg ctgctgtggg ccggggttga tcgactcgtg
     7981 gccgtgtacg acgcgccaga cgccgccctg ccgaacgtag acctgcgtga ccttgcccgt
     8041 gtactgcatg ggccggcccc cgatatcgac ggtcgcgccg acgttgtagg tgaggatggc
     8101 cgtgtcgccg taggtctgca cgcgcggggt gtacatctgc cagtccacca tcttgaagcg
     8161 ctcggcgaac tgctcgaact tcggcaggaa gttctccttc gggtcggtct ccagataggg
     8221 actcatcggg ccgaagtagg tggagtcggc ggccatgtag tcggcgaaga atgccttgtc
     8281 tttgcgctgc cacgcctcgg cagacgcctt ctcgcgggcg atgatggcgc gcgcgatctc
     8341 atccttgctc tcgggttcgg cggcgctgcc ggtcgaggcc gtgacgccat agccggcggc
     8401 gaagaacaac agcacgagga ggcccaggga gagacggttt ttcatcacaa tactccttgc
     8461 gtttggcggg cgcgggggcc gcttgcgagg ctcccgctgc ggttgatcgt cggcgaggtg
     8521 gcgggtgaac agccgtcaat cgtgaatcgt caatcgcggt cgaagggcga ccgagcttga
     8581 gtggtgaatc gcaagtctga tttgtctcaa cccgacgatt gacgattcac gattgacgat
     8641 tgacggcttt aagaactggc gcccttgagc gtgatgagcg agatgttggc gcccgtcggg
     8701 tcggtgatga cgcagaagcg gccggtgttg gggatgtcca tcggcggcac agagaccttc
     8761 ccgcccaact cctcgacgcg cttcgcggag gcgtccacgt catcgacggc gacgtaggac
     8821 gaccatcggg tgggcatctc gccgccgcac tccggcccgg cctgatagat gccgccgacg
     8881 tgcttgccgc cgacgacgat ctcgttgtag accatgccgg ccccgtcgga ggtctttatc
     8941 tcccagccca aaagctcggt gtagaatttc ttggcggcct cggcgtcttt cgtggacagt
     9001 tcggtccagc agaaggtccc gtgggccggc atctcgtggt gttggtcagc catcgcagta
     9061 gtttctcctt cagtaagatt caggtttgag gactcctcgc gcggcccgcg ggccacgcga
     9121 gaaaaaagaa aggtgagccc cctcacaaag ttgactcacc tttcaaccca ccccttgctc
     9181 gttcctgcct tgcgagggcc tgcttgtgtt ccttcttaaa acttgttcgg ctcttaatgc
     9241 gcgacgagcc attcactaaa aattttcgac tcttcgagct tgccgctcca gcgcgcgcgg
     9301 ttcttgcccg tcagcgcgat gaccatcgcc tggtgctgcc cggcgcggtc gccgaggcgc
     9361 tcgaagtagt tgtaaagacg gtcgatcttc tccaccgact cgcgcgccgc atccattgat
     9421 ttgaacgaca gcacgttgcg gaagaaccgc gtgtagacgt tctgctcggc gccggtggtc
     9481 gtgtagaggt cgaagtagcc ggagaggtgt ccggccgtct gcaacacctg cgcgacgtaa
     9541 gagggccgcg cgtgaacctt gcggacgatc tcggcgacat cggtcgtgcc cgactcgtag
     9601 aggtctaaaa tctcgtcctt cttcgagaag ggcagctcga tgcgcccctc gcgctcgctc
     9661 gcccgctcgg ccgttcccac gccagcacgc ggctcgtcgg cgtattcgag ttcgagcgaa
     9721 atcggcgcgt gctcctgaat gatttcttcg gtcaacatct ccttaacctc cttgtcaggg
     9781 gagtgagcgg tgagccgcta agcagtttgg ttttggcttt tactgcttag cggctcaccg
     9841 cttactttct tcagctcgcg cgacgcatgc cgccagcggc ctccgtctgg gcgcccttga
     9901 ggtggtcgat gaagctggaa gcggcgcgct tcgacaactc ctcggtgcgg cagcgcatct
     9961 gcgcctggca ctcctcctcg gcgtccacgc cggcctcgcg agcgagcgcg cggatcatgc
    10021 cgagctgctt cggcgtgacg aggtcggcca tcgacttggc gagcgggtcg cgcgggaagt
    10081 cgccgggctg cggccccgcg ccgtcgcgct cgatcacttc cgactcgcgc tggtagagtt
    10141 cgcgcgcgat gccgaacttg atggcggcgc gcttcagcgc gtcgtgctcg gccttcttga
    10201 tgcccgtctc gttgtcggcc gtgcccgtgc cgacgccctc gcgcgtgacg ccgtccaccg
    10261 tgatggcgca cgtcaccgcg accatgtcgc cgatctggac gatgttgcgc accgagtgcg
    10321 accaggtggg cgcgacgcgg tcgaggatat cggccacggt gtgccactcg acgtactcga
    10381 ccatgtgacg gttgccgttg cggtcagtcc agccctcgcg cgtcttcacg agcttggcgt
    10441 ccaccggctg gcgcagcacg cgcagcgtgt tggtgaaagc catcgggacg accgacgccg
    10501 acgacgcgtg ctcgaactca ccctcgaaat cctgagccac ttccggctcc tgcacgttag
    10561 cggttgctct cttgctcatc tcctgatgct ccttttcgtc ttcgtgtgtc gcgagcgaac
    10621 ccgtgagcag aatggcgtcg gcgggggcgt cgttcttcgt cgcctccact tcgtcctgcg
    10681 gctcgggggt ttgctcccgt acgaaacgcg gctggcggct ggcggccgtt ctcacggtcg
    10741 cctcggtcgc ttcgtcgcgc ccctcggatg cgtttttgtc ttgcgtgttg ggcgtctccg
    10801 ccaaaacgtt cacagtcgaa atccccgcgc ccgcctgagc gaggggtctg ttcgcggcct
    10861 gcttggtgac cggctcagaa ctcttgttga gaagcgagtg cttgacggcg aggatgtgct
    10921 cgcaacggaa cgtcggctcc ccggccgaca gctcctcgaa ctccagacag gagcagcgca
    10981 cgtgcccggc ctcgtcgcgc cagacctcgt aggccgcgcg cttgccgcgc aaggaaggcg
    11041 tggtgacgtg gaagcggtcg ccctcgcggt tgaccagacc catcgcggcg atgccttgcg
    11101 cccgcttgtc ccgcatgatc gctttcttgt ccagaacggc tgccattgcc ttaccctccc
    11161 gaaaacttca aaacttacgc cgcgcgctcg accggaagct ccgtcaccat gacgcgctcc
    11221 gtcgaatgct tctccgcgcc gctgccatcg cgctcctgca agcgccgctc aaggctggcg
    11281 atacgcgtct ccagctcctc gaccttccgg ctctgccggt agaccgtctt aaaaagcccg
    11341 tcaaacgtct cccagatgtg ttccacgttg cttctccccg tgcctttgta cccacagtcg
    11401 cggccactcg tttcgccgct ctgtttcata cgtgaaacag tgtaccatgg gttcccggtt
    11461 gttgcaagca taaaacacag tcttgcatct gggatttaag ttgtgctatg gtgcgccttg
    11521 gaaggtgggc gtgcccgccc gttttaggct gggccgcgaa gccccgaagg agataggaac
    11581 atggcagata atgcgaataa tctagtaaat acggtggaac tggggcgggc catccgccgc
    11641 aagcgtgagg acgcgggtct gagtctgcgg gatgtcgcgg atgaaacggg cgtgtcggcc
    11701 tcgacgctgt cgcgcatcga gaacggcacg ggcaagcccg acgccgacaa catcgcgcgt
    11761 ctcacgggct ggctcaacat gcccatggag cgtgtcatgg gcaaccgcgc cgccgccggc
    11821 gacctggagg acaaggccgt cgtctacttc cccaaagagg ccatgcccga catcgtcgaa
    11881 gcgcacctga gagccgaccg caacctcacg cccgagaccg ccaaggccct ctcggaactc
    11941 ttccgcgtcg cctaccagca gttttcacat cccggcgagg acacacgcgg gcgcaataag
    12001 cggaagtagc agcaggcacg ttttaggacc gacacttatt aggcgctcga tccccgtggg
    12061 ttgagcgtct attctgttta gagagaaggc gagtataagc taaaaagatt ggtggaaagg
    12121 gatggaagta tgagcaccag aactttacgc gcccattttg acggcgagca ggtatgtctg
    12181 gatgaacccg cccagctgga gccgggaacc aggcttatca tcacggtcct gccgggtagg
    12241 attttagata acggcgatga ggattggtcg ctcctctcgt tggaaggatt ggccggcgcc
    12301 tacggcaagg atgaacctga ataccctact gagctaatta aagaattgaa tcccaactat
    12361 gaaggaaggt gacttaatcc tcacgccgtt ttcgcaggca gacggggccg tgaagaatcg
    12421 tccggctgtc gttttgcggg agttgccgcc ttatcgtgac ctgctcgtct gcggcgttag
    12481 cacaaaaatc aacaggtgaa agactttgac gatatcatcg ccccgacgga tgcagacttc
    12541 ccgtcgagtg ggctagtgtc ggagtcaata atacgtctcg gttttctggc tgtgcttccg
    12601 cgaaaaagag tcataggcgc aatcggatca atttcatctg accgtcacaa acgtcttcta
    12661 aaaatattgt cggcttatct gaccgcgaac atcgccgact aaaacccttc aacaccaagt
    12721 acaaccgact cctcgctttg ggccggaacg acgtgcgtca atccggcggc tatctgaaac
    12781 tcccgatcag atcaaacctg tataggagaa aatcatgtcg gacaaagtga aacccatccc
    12841 cgagggctac cacagcgtga cgccttacct gtgcgtgagc gacgcggccc gcgccatcga
    12901 gttctacaag gaggcgttcg gcgcaaccga ggtcgggcgc atggaggcgc ccggcgggag
    12961 gatcgggcac gccgagatca agatcggcga ctcgcacgtc atgctcgccg acgagtttcc
    13021 cgaaatcaac ttccgcagcc cgcagacgct gggcggcccg actgctcaat tcatgatcta
    13081 cgtcgaggac gttgacgcgc gcgtcgagca ggcggtcgcc gccggcgcga agctggtgcg
    13141 gcccgtcaag gatcagttct acggcgaccg cagcggcggc gtggaagacc ccttcggcca
    13201 cttctggtac atctccacgc gcatcgagaa cctgacgcat gaagaggtga agaagcgcag
    13261 cgacgccgag ataacgcaac acgcgggagc atgaagcgcg actgacc
//
LOCUS       DASVES010000225        11914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1565802, whole genome shotgun sequence.
ACCESSION   DASVES010000225 DASVES010000000
VERSION     DASVES010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565802"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..854
                     /locus_tag="VF240_09155"
     CDS             255..854
                     /locus_tag="VF240_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175413.1"
                     /translation="MKLALILLCGVAALLARAPEAAAQKAATAAAPAAIKHGGKIEMK
                     YDGFNLETVVALRKMQVTCGSGKGFKDISKDVCVSLAVWLHCPGQQLDFVRRARVQLI
                     FEAEEWDRRHTPAERGLVVVADGETLRLGSMSLVSNEVGSGLLVDKSKEVLEISIPYD
                     AFVKIARADSVEMRVGADAFPLRDKNVAALRDLNSRVKP"
     gene            complement(945..2420)
                     /locus_tag="VF240_09160"
     CDS             complement(945..2420)
                     /locus_tag="VF240_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX7175414.1"
                     /translation="MSDTPDGDDDDTSRTSAPAASDARRFTTPVKWGVVVFSALAVAA
                     APVPSGITPQSWRLLAIFVGTIVGSIVRPVPGGAMVLIGVTALVLTGTLTATQALSGY
                     GDPVAWIALAAFILSRGMIKTGLGRRIAFNFIRLIGHRSLGLGYALVTTDLLLATVIP
                     SNGARCGGIIFPIGKSMAEAYGSEPGPTANRLGAFLLPFIYQCDVIICAMFLTGQAGN
                     LLIARFAAETAGVELSYGRWALAALLPGLVALAVVPPLLYKLFPPEITHTPAAAALAR
                     EELRRMGPMARGEKLMLVVFAFVAALWATTALHEIHYSMVGLLGVCALLLGGVLEWED
                     VLAERTAWDVFVWYGGLLMMAGALSETGIIKRFAESSAEFTVGWAWWAALGVLLLIYF
                     YAHYAFASISAHATAMYIPFLVVVIAAGAPAPLAALSLAFFSNLSASLTHYGTTPAPI
                     FFGAGYVKQRRWWLIGLVASVPNILVWTTVGLLWWKLLGWW"
     gene            2712..3131
                     /locus_tag="VF240_09165"
     CDS             2712..3131
                     /locus_tag="VF240_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175415.1"
                     /translation="MSLAVKLSVAAAGLYLLAGMLLGIIKYRRIMTSAEHRAPVYIDI
                     AHRAAFMYSFAALVIARLLESSPYSERVQVGAAGLVLAYFTVTILGYTAHGLRDDTEN
                     IFAERNVTTTWFMYTLIAAEVGGLSVILWGFLSTQFF"
     gene            3152..4111
                     /locus_tag="VF240_09170"
     CDS             3152..4111
                     /locus_tag="VF240_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7175416.1"
                     /translation="MMETIVFLDRNTLRADVRRPAFEHRWVEYEKTRGEEVVERLNDA
                     TIAITNKAPLRREALARLPRLRMIAVAATGVDIIDLDAARERGLVVSNVRGYAQWVVP
                     EHVFALILALRRNLLAYREDVRRGAWAAADAFCLLDHPIRDLRGSTLGIVGHGSIGRG
                     VERLARAFGMRVLIGEHKGAREVREGRTAFAELLRESDVVSLHAPLSAETRNLIGADE
                     LRLMKRTALLINCARGGVVDEAALAAGLREGLIAGAGVDVLSREPPRDGSPLLELDLP
                     NLIVTPHVAWASREAMQALADQLIDNIEAFIRGEPRNTVISDK"
     gene            complement(4165..4503)
                     /locus_tag="VF240_09175"
     CDS             complement(4165..4503)
                     /locus_tag="VF240_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175417.1"
                     /translation="MSENDNQTAGASGEDATAAAADSAPQTEDASAQATTEQDTTGQG
                     ATEQGEAGQAAAEEPPASTEEITYHAVEKDGQVTAIWEMQGRKHLRTIDPRSRQGEEI
                     LRLYTSEHQE"
     gene            complement(4616..5986)
                     /locus_tag="VF240_09180"
     CDS             complement(4616..5986)
                     /locus_tag="VF240_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7175418.1"
                     /translation="MSEAITADPLARHSDVAAGRPVCPACGRESADGLVTFDSLSDDL
                     KRIATANSPGDGPVSHVCPRCVELFERASRQLRKYETVFEQGRFVLPTWLRLGADERF
                     TGRGVTMALLDSGFYAHADLTEPVSRILAYHNISPQGTGTLDSIDVASWHGMMTSVVA
                     AGNGHLSDGFFRSIAPAANLVLVKVSSSGHISEGNIMRGLEWVLDNREKFRIRVVNIS
                     AGGDEPASYLHNPLSRTIEQAVAEGLVIVCAVGNSGHIPGHPVLPPASSPACIAVGGL
                     DDRNSLDRARRGMYRSSYGPTVDGLQKPEVIAPGIWVPAPILPHTPTAAEADLYATLD
                     RAEDKELVALIEEHAEVDKDLHESRDLPLHLLRQMISIKLREADVISEHYKFVDGTSF
                     AAPIVSSTVACMLEANPHLTPSHVKRILIETAERLPGIEIDRQGWGVVEPRKAVQMAL
                     ALRPNE"
     gene            6165..6548
                     /locus_tag="VF240_09185"
     CDS             6165..6548
                     /locus_tag="VF240_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175419.1"
                     /translation="MESSIQIIAGVSFLVVGLSHLFRPRVWAQFFIDLRERGEVASFH
                     VALIHLPLGALIVGFHNVWRGAGLVLTLIGWAYILKSLVYFLWPRYGLRALARVSVER
                     AWEFAAGGVLLAAYGGFLLYTAFRG"
     gene            6712..7143
                     /locus_tag="VF240_09190"
     CDS             6712..7143
                     /locus_tag="VF240_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175420.1"
                     /translation="MSQEQPTVPGIFRRAQAMLAENPALSMKDLRSRLAGEYRGAAMP
                     SLLTLAIPEQDARAPEEDWSAGLSIIARGIQQENWGEIINGILMCLDQTVRFERERGQ
                     LGSADDWHDRTVGIRGQMEKGVGKWMPDELMQLAERATKKR"
     gene            7230..7922
                     /locus_tag="VF240_09195"
     CDS             7230..7922
                     /locus_tag="VF240_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX7175421.1"
                     /translation="MSFLNSIFKDPKYQREGPRRPEEVAEPDVIVSPDTRREQRLPPG
                     QTRTRKWPVLDAHGTPDVDLETWEFEVTGLVERPLKWSLDEFMQLPAVKVYADFHCVT
                     RWSRLDNVWGGVATRTVAELVGLKPEVKFVVAHAHDYGWTTNLPVEHFLAEDSLFAWS
                     HDGEPIPREHGGPVRLIVPQLYAWKSAKWVKGVTFLAADRAGFWEEGGYHMRGNPWQG
                     RDGERFRHQGNE"
     gene            8071..9633
                     /locus_tag="VF240_09200"
     CDS             8071..9633
                     /locus_tag="VF240_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7175422.1"
                     /translation="MKDAVINQHPAARQPNQPAAPKDAAAVILVREGTDARDPEIFWA
                     RRGDALVFLPGFYAFPGGQRDAADAEVSVRGAEDADASAMIVCAARELLEETGVLVAR
                     GAETLTRGQLASVLDDLTSGRMTFNELLAHYGLNLDAADFRFVGRWVTPPFSPRRFDT
                     LFFLVRCPRKQEPRVLSEEFSEGAWAKAGDALARWQRAEVLAVPPVIHALRTLASGMT
                     DDLVERFLGVPQAHGQPVRRIEFLPGFVCFPLRTPTRPPATTTNCYVVGCGGFVVIDP
                     ASPYEDEQRELHAYIDELLAAGRSLREIILTHLHPDHVGGAEALREHLGGRVTVAAHR
                     LTAEPLEGRVRVDRLIEDEEVIELEGEPRLSLRAMHTPGHTRGHLSFYEERTGALLTG
                     DNIVGIGSVLIDPPEGDVRAYLSTLERFRALPHLKILLGGHGPAVGAPRAKIDEYIRH
                     RLERERDILAAVREGASSPVEIVARVYADVHPKMHAMAERAVVAHLEKLHADGLVALA
                     ADGDAAPRARPV"
     gene            9726..11123
                     /locus_tag="VF240_09205"
     CDS             9726..11123
                     /locus_tag="VF240_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7175423.1"
                     /translation="MRQKETSQKKDRAPAAASVATAVGGAAGASAEESRHERERLAAL
                     NSDIGIALVQGDTLSETLHRCAEALVKHLDAAFARIWTLNEEEQALELRASVGLYRHL
                     DGPHSRVPVGKFKIGLIAEERLPHLTNSVVGDPRVGDQEWAKREGMVAFAGYPLIVEE
                     TLVGVMAMFARHALSENDLQAMAAIANGVALGIKRKQAEGALRESEELNQAVLNSLTA
                     HIAVLDGDGRIVAVNDAWRRFAEENEGAGGHAVQGANYLHVCTQAAGGEPDDGGAVLA
                     GIESVLKGARDSFTMEYACHSPTEERWFLLHANPLPSERGGAVVSHINITERKRTEET
                     LARLSRERETMLEEVSTPLVPVLEGVLVMPLIGTLDTARMQRATQAALTEVRRTAARA
                     CVIDITGARITDSHAVSNLTNLVQSLRLVGAEAIITGVGADAARSIVGLGLDLRGLRT
                     HRTLAQALATLRRDE"
     gene            11235..>11914
                     /locus_tag="VF240_09210"
     CDS             11235..>11914
                     /locus_tag="VF240_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175424.1"
                     /translation="MLPATLGELKRQGYRVRPVKDELRANLIEKLRAGERLFPGIVGY
                     DETVVPQITNAVLARHNLILLGLRGQAKSRIIRQLVGLLDERVPAIEGSEVNDNPFRP
                     ISAYGRQLVEREGERTPIVWIEREARFVEKLATPDVTIADIIGDIDPIKAARGGHLLS
                     DELTIHYGLLPRANRGLFAINELPDLAGKIQVGLFNIMQEGDVQIKGYPVRLPLDVML
                     IFSANPED"
BASE COUNT         2050 a         3946 c         3933 g         1985 t
ORIGIN      
        1 ctctgcgcct ctgcggtgaa tctgtcttca tacccgctca actgaaagcg gctataagat
       61 ttggcgcgga ctcacaacgc cccgcgacat taccgttgac acgccgacgg gggcgtccct
      121 atattaattt caggcggccc ggccggtgcg cgctagccag tgtgtttgac cggccgcctc
      181 ttttgccctc cgaaattttc gcctcacccc gaccgccgcc gccggcaatc ccaggattac
      241 caagaggtcg atttatgaaa ctcgcgctca tactcctgtg cggcgtcgcc gcgctactcg
      301 cacgcgctcc cgaagccgcg gcccagaagg ccgcgaccgc agcagccccc gccgccatca
      361 agcacggcgg gaagatcgag atgaagtacg acggcttcaa tctggagacg gtcgtcgcgc
      421 tcaggaagat gcaggtcacc tgcggctcgg ggaaaggctt caaggacatc tcgaaggacg
      481 tgtgcgtcag cctcgccgtc tggctccact gtccgggcca acagctcgac ttcgtccgcc
      541 gcgcgcgcgt gcagcttatc ttcgaggctg aggagtggga ccgccgccac acgcccgccg
      601 agcgcggcct cgtggtcgta gccgacggcg agaccctccg cctcggctcg atgtcgctcg
      661 tctcgaacga ggtcggctcg ggtctgctcg tcgataagtc gaaagaggtt ctggaaatct
      721 cgattcccta cgacgccttc gtcaagatcg cgcgcgccga ctccgtcgag atgcgcgtcg
      781 gagcggacgc cttccctctg cgcgacaaga acgtcgccgc gctgcgcgac ctgaacagcc
      841 gcgtcaaacc gtaaaagaaa gtgatgagtg ataagtgata agtgataagt gaaagcatct
      901 gtcttttctt atcacttatc actcatcact catcactttt cgtttcacca ccagccgagc
      961 agcttccacc agaggaggcc gaccgtcgtc cagacgagga tgttgggcac ggaggcgacg
     1021 agaccgatga gccaccagcg gcgctgcttg acgtagccgg cgccgaagaa gatgggcgcg
     1081 ggcgtggtgc cgtagtgggt gagcgaggcg ctcaggttcg agaagaaggc cagcgagagc
     1141 gcggccagcg gggccggcgc gccggcggcg atgacgacga cgaggaacgg gatgtacatc
     1201 gccgtggcgt gcgccgagat gctcgcaaac gcgtagtgcg cgtagaagta gatgagcagc
     1261 aggacgccga gcgcggccca ccacgcccag ccgacggtga actcggccga cgattcggcg
     1321 aagcgtttga tgatgcccgt ctcgctcaag gcgccggcca tcatcagcag gccgccgtac
     1381 cagacgaaca cgtcccacgc cgtgcgctcg gccagcacgt cctcccattc gagcaccccg
     1441 ccgaggagca gcgcgcagac gccgagcagt ccgaccatcg agtagtgtat ctcgtgcagc
     1501 gccgtcgtcg cccacagcgc ggcgacaaag gcgaagacca cgagcatcag cttctccccg
     1561 cgcgccatcg gccccatgcg ccgcagctcc tcgcgggcga gtgcggccgc ggccggcgtg
     1621 tgggtgatct ctgggggaaa gagtttgtag agcagcggcg gcacgacggc gagcgcgacg
     1681 aggcccggca aaagcgccgc cagcgcccag cggccgtaac tcagctcgac gccggccgtc
     1741 tccgcggcga accgggcgat gaggaggttg ccggcctgcc ccgtgaggaa catcgcgcag
     1801 atgatcacgt cgcactggta gatgaagggc aggaggaacg cgccgagccg gttcgcggtc
     1861 ggccccggct ccgagccgta ggcttcggcc atgctcttgc cgatggggaa gatgatgccg
     1921 ccgcagcgcg cgccgtttga cgggatgacc gtagcgagca gcagatcggt ggtgacgagc
     1981 gcgtagccga ggccgagcga gcggtgcccg atgagacgga tgaagttgaa ggcgatgcgg
     2041 cggcccaagc cagttttgat catcccgcgc gagaggatga aggcggcaag cgcgatccac
     2101 gcgacggggt cgccgtagcc ggagagggcc tgcgtggccg tgagcgtgcc cgtgagcacg
     2161 agggccgtca cgccgatgag caccatcgct ccgcccggca cggggcggac gatggagccg
     2221 acgattgtgc ccacgaagat cgcgagcaga cgccacgact gcggcgtgat cccactgggg
     2281 acgggcgcgg cggcgaccgc cagcgcggag aagaccacga cgccccactt gactggcgtc
     2341 gtgaagcgtc gcgcatcgct cgccgccggg gcgctcgtcc gagacgtgtc gtcgtcgtcg
     2401 ccgtcaggcg tgtcgctcat gtgtgtcgat ggagaaaggt ttcggaatcg tagagccgcg
     2461 cgccggatgc taacaccgcg cggcgcacgg ctcaagattt ttagtttgtc agttgttagc
     2521 cattagccat tagcggttag ccggaagacg gcgaaccggc gattcattta ttcaccggcg
     2581 gtgcttcgtc tttagctcct tctgctggct aaccgctaat ggctaatagc tgaccgctct
     2641 ttaacccgaa cggtgttacc atgcgacgcc gcctcgcccg tcagcgtccg caagcatgga
     2701 agagaaatct gatgagcctt gccgttaagt tgagcgtcgc cgccgcgggc ctgtacctgc
     2761 ttgcggggat gctcctcggc atcatcaagt atcgacggat catgacgagc gccgagcacc
     2821 gcgcgcccgt ctacatcgac atcgcgcacc gcgccgcctt catgtacagc ttcgccgcgc
     2881 tcgtcatcgc ccgcctcctc gaatccagcc cctactccga gcgcgtgcag gtcggcgccg
     2941 ccggactcgt gctcgcgtat ttcaccgtca ccatcctcgg ctacaccgcc cacggcctgc
     3001 gcgacgacac ggagaacatc ttcgccgagc gcaacgtgac gacgacgtgg ttcatgtata
     3061 cgctcatcgc ggccgaggtc ggcggcctca gtgtcatcct ctggggcttc ctctccacac
     3121 aattcttcta actcgacgaa gcaacatcta catgatggaa accattgtct tcctcgaccg
     3181 caacacgctg cgcgccgacg ttcgtcgccc ggccttcgag caccgctggg tggagtatga
     3241 gaagacgcgc ggcgaggagg tggtcgaaag gctcaacgat gcgaccatcg cgataacgaa
     3301 caaggcgccg ctccggcgcg aggcgctcgc gcggcttccc cggctcagga tgatcgccgt
     3361 cgccgccacc ggcgtggaca tcatcgacct cgacgcggcg cgcgagcgcg gcctcgtcgt
     3421 ctcaaacgtc cgcggctacg cgcaatgggt cgtccccgaa cacgtcttcg cgctcatcct
     3481 cgcgctgcgc cgcaacctgc tcgcctatcg cgaggacgtg cgccgcggcg cgtgggccgc
     3541 ggccgatgcg ttctgcctcc tcgaccatcc catccgagat ttgcgcggca gcacgctcgg
     3601 catcgtcggc cacggctcca tcggacgcgg cgtcgagcgc ctcgcccgcg ccttcgggat
     3661 gcgcgtcctc atcggtgagc acaagggcgc gcgggaagtc cgcgaggggc gcaccgcctt
     3721 cgccgaactt ctgcgcgaga gcgacgtggt cagcctgcac gcgcctctga gcgcggagac
     3781 gcgcaacctg attggcgcgg atgagctgcg attgatgaag aggacggcgc tgctgattaa
     3841 ctgcgcgcgt ggcggggtgg tggacgaggc ggcgctggcc gctgggttgc gtgaaggact
     3901 aatcgcgggc gcgggtgttg acgtgctcag ccgcgagccg ccgcgcgatg gcagcccgct
     3961 gttggaactc gatctgccga acctgatcgt cacgccccac gtcgcctggg cgagccggga
     4021 agcgatgcag gcgctcgccg atcaactcat cgacaacatc gaggcgttca tccgcggcga
     4081 gccacgaaat acggtgataa gtgataagtg atgagtgata agaaagagaa agacagagtt
     4141 tgtcttatca cttatcactt atcactactc ctggtgttcc gacgtgtaga gccgaaggat
     4201 ttcttcgccc tgacgggagc gggggtcgat ggtgcggagg tgcttgcggc cctgcatctc
     4261 ccagatggcc gtcacctgtc cgtccttctc gacggcgtgg taggtgatct cctcggtgct
     4321 ggcgggcggc tcctcggccg ccgcctgccc ggcttcgcct tgctcggtcg ctccctgccc
     4381 ggtcgtgtct tgctcggttg tcgcctgcgc gctcgcgtct tcggtctgcg gcgcggagtc
     4441 ggcggcggcg gccgtcgcgt cctccccgga cgcaccggcg gtctggttat cgttctctga
     4501 catcgaagca atccctctct tggtagtttg atttaattct tgatgtgatt tgcacgcgga
     4561 ttgtggcaca cgcccgccgc gcgtgtctag cgacagggtc tcggcgcctt cctcatcact
     4621 cgttcggacg cagggccagc gccatttgga cggccttgcg cggctcgacg acgccccagc
     4681 cctgccggtc gatctcgatg ccgggcaggc gctcggccgt ctcaatcaaa atccgcttga
     4741 cgtgcgacgg cgtcaggtgc gggttagctt cgagcatgca ggcgaccgtc gaagagacga
     4801 tgggcgcggc gaacgaggtg ccgtcaacga acttgtagtg ctcgctgatc acgtcggcct
     4861 cgcgcagctt gatcgagatc atctggcgca gcagatgcag cggcaggtcg cgcgactcgt
     4921 ggaggtcttt atcaacttcg gcgtgctctt cgatgagcgc gacgagttcc ttatcttccg
     4981 cgcggtcgag cgtggcgtag aggtcggcct cggcggccgt gggcgtgtgc ggcaggatgg
     5041 gcgcgggcac ccagatgccg ggcgcgatga cctcgggctt ttgcagtccg tcaacggtgg
     5101 ggccgtagga ggagcgatac atgccgcggc gggcgcggtc gagcgagttg cggtcatcaa
     5161 ggccgccgac ggcgatgcag gccggcgacg acgcgggcgg cagcacggga tggccgggga
     5221 tgtggccgga gttgccgacg gcgcagacga tgacgaggcc ctcggccacg gcctgctcga
     5281 tggtgcgcga cagtgggttg tggagatagg aagccggctc gtcgccgccg gccgagatgt
     5341 tgacgacgcg tatccggaac ttctcccggt tgtctaagac ccactccagc ccgcgcatga
     5401 tgttgccctc ggagatgtgg ccggagctgc tcaccttgac gagaacgagg ttggccgcgg
     5461 gcgcgatgct gcggaagaag ccgtcggaga gatggccgtt gccggcggcg acgaccgagg
     5521 tcatcatgcc gtgccagctc gccacgtcga tggaatcgag cgtgcccgtc ccctgcggcg
     5581 agatgttgtg ataggcgagg atgcggctta caggctcggt caggtcggcg tgcgcgtaga
     5641 agcccgaatc caacaacgcc atggtcacgc cgcggcccgt gaagcgttcg tccgcgccga
     5701 ggcgcagcca ggtggggagc acgaagcggc cctgctcgaa aacggtctcg tacttccgca
     5761 actgcctgct cgcgcgctcg aagagttcga cgcagcgcgg gcacacatgc gagacggggc
     5821 cgtcgcccgg cgagttggcc gtggcgatgc gcttgaggtc gtcggacaga gagtcgaacg
     5881 tcacgaggcc gtccgcggat tcgcgcccgc acgcgggaca cacgggacgg cccgcggcca
     5941 cgtcagaatg gcgcgccaaa gggtcggcgg tgatggcttc agacacggat aacttttccc
     6001 cttgcgaatt cgtttgattg gtttcggttc ggtctatgat gtattcagat gagggtcgga
     6061 cgcaagacgc gaacgctcct ttagagtgat ttacttgagt gagggcgaaa atagattcac
     6121 cgcaaaggcg caaaggacgc ggagggcgcg cggagggtaa atttatggag tcgtcgatcc
     6181 agattatcgc cggcgtcagt tttctcgtcg tcggcctctc tcatctgttc cggccgcggg
     6241 tctgggcgca gttcttcatc gatttgcgcg agcgcggcga ggtcgccagc ttccacgtcg
     6301 ctctcataca cctgccgctg ggcgcgctca tcgtcggctt ccacaacgtc tggcgcggcg
     6361 cgggtctcgt cctcacgctc atcggctggg cctacatcct caagagcctc gtctacttcc
     6421 tctggccccg ctacggcctg cgggcgctgg cgcgcgtctc ggtcgaacgg gcgtgggagt
     6481 tcgccgccgg cggcgtgctg ctcgccgcat acggcggctt tctcctctac acggcctttc
     6541 gcggttgacg gcgcgtcggc cgagcgaacg tctccggcat ccacccggac acggcaccag
     6601 cgcgtcgctt tctgctgtgt cgcgccgtcc ggtagccctc gccgccggga tggtataaat
     6661 tactctcaaa ccgaaacccc cttgatgtcc gttgaagagg tctcgaagac gatgtctcaa
     6721 gagcaaccga cggtgcccgg catcttccgg cgcgcgcagg cgatgctggc cgagaacccg
     6781 gccctttcga tgaaggattt gaggtcgcgc ctcgcgggcg agtatcgcgg cgcggcgatg
     6841 ccgtcgctgc tcacgctggc gatcccggag caggacgcgc gcgcgccgga ggaggactgg
     6901 tcagccggtc tctccatcat cgcccgcggc atccagcagg aaaactgggg cgagatcatc
     6961 aacggcattc tcatgtgtct cgaccagacg gtgcgcttcg agcgcgagcg cggacaactc
     7021 ggctcggccg acgactggca cgaccgcacc gtcggcatcc gcggccagat ggagaagggc
     7081 gtcggcaagt ggatgccgga tgagctgatg cagctcgcgg agcgggcaac gaaaaaaagg
     7141 tgataagtga taagtgataa gtgatgagac gaaaactgtc tttctctttc ttatcactta
     7201 tcactcatca cttatcacgg ctctgaagaa tgagtttcct caacagcatc ttcaaggacc
     7261 cgaagtacca gcgtgagggg ccgcgacggc cggaggaggt ggcggagcct gacgtgatcg
     7321 tcagcccgga cacgcggcgc gagcagcggc tgccgcccgg tcagacgcgg acacgcaagt
     7381 ggcccgtgct cgacgcgcac gggacgccgg acgttgacct tgagacgtgg gagtttgaag
     7441 tgacgggcct cgtcgagcgg cctttgaagt ggtcgctcga tgagtttatg caactgccgg
     7501 cggtcaaggt ctacgcggac tttcattgcg tgacgcgttg gtcgcggctc gacaacgtgt
     7561 ggggcggcgt ggcgacgcgc accgtggcgg agctggtcgg cttgaagccc gaagttaagt
     7621 tcgtcgtcgc ccacgcgcac gactacggct ggacgacgaa ccttcccgtc gagcatttcc
     7681 tcgccgaaga ctcgctcttc gcgtggtcgc acgacggcga gccgataccg cgcgagcacg
     7741 gcggccccgt ccgtctcatc gtcccacagc tctacgcgtg gaagtcggcc aagtgggtca
     7801 agggcgtcac gttcctcgcc gccgaccgcg ccggcttctg ggaggaaggc ggctaccaca
     7861 tgcgcggcaa cccgtggcag gggcgcgacg gcgaacgctt ccgacatcag ggcaacgaat
     7921 gaatccgggc agagaaatca gaagtgatga gtgatgagtg atgagtgatg agaaaaggca
     7981 agttgctttt actcatcact catcacttat cactgctttt atcctgccca tccatgttaa
     8041 tttttctttc ctgacagaga gcttaacgcg gtgaaagacg ctgtcatcaa ccaacacccg
     8101 gcggcgcggc agccgaatca gccggcggcg ccgaaggacg cggcggccgt catcctcgtg
     8161 cgcgaaggca cggacgcgcg cgaccccgaa atcttctggg cgcggcgggg cgacgcgctg
     8221 gtcttcctgc cgggctttta cgccttcccc ggcgggcagc gcgacgcggc ggacgcggag
     8281 gtgagcgtcc gtggagcgga ggacgcggat gcgtcggcga tgatcgtgtg cgcggcgcgc
     8341 gagttgttgg aggagacggg cgtgctcgtc gcgcgcgggg ccgagacgtt gacgcgtggg
     8401 cagctcgcct ccgtgctcga cgatctcacg tcggggcgca tgaccttcaa cgaactgctc
     8461 gcacactacg gcctcaatct cgacgccgct gacttccgct tcgtcggccg ctgggtcacg
     8521 ccgccgttta gcccgcgccg cttcgacacg cttttcttcc tcgtccgctg cccgcgcaag
     8581 caggagccgc gcgtgctctc cgaagagttc tccgagggcg cttgggcgaa ggctggggac
     8641 gcgctcgcgc gctggcagcg ggccgaggtg ctggccgtgc cccccgtgat tcacgcgcta
     8701 cgcacgctcg cttcggggat gacggacgac ctcgtcgaga gattcctcgg cgtgccgcag
     8761 gcgcacggcc agcccgtgcg ccgcatcgag ttcctgcccg gcttcgtctg cttccctcta
     8821 cgcacgccga cgcggcctcc ggccacgacg accaactgct acgtcgtcgg ctgtggcggg
     8881 ttcgtcgtca tcgaccccgc ctcgccttat gaagacgagc aacgggagct gcacgcatac
     8941 atcgacgagc tactcgccgc cgggcgctcc ttgcgcgaaa tcatcctgac gcacctgcac
     9001 cccgaccacg tcggcggcgc ggaggctctg cgcgaacatc tgggcggacg cgtcaccgtc
     9061 gcggcgcacc gcctgacggc cgagccgctg gagggccgtg tccgcgttga ccgccttatc
     9121 gaagacgagg aagtgatcga actcgaaggc gagccgcgcc tctcgctgcg cgccatgcac
     9181 acgccggggc acacgcgcgg ccacctcagc ttctacgagg agcgcacggg ggcgcttttg
     9241 acgggcgaca acatcgtcgg catcggctcc gtcctcatcg acccgcccga gggcgacgtg
     9301 cgcgcgtacc tctcgacgct cgaacgcttc cgcgcgctcc cacatctcaa gattctcctc
     9361 ggcggccacg gccccgccgt cggcgccccg cgcgcgaaga tcgacgagta catccgtcac
     9421 cgtctggagc gcgagcgcga catcctcgcg gccgtgcgcg agggcgcgtc gtcgccggtc
     9481 gagatcgtcg cgcgcgtcta cgcggacgtg catccgaaga tgcacgcgat ggccgagcgc
     9541 gccgtcgtcg cgcacctcga aaagctccac gcggacggcc tcgtcgcgct cgccgccgac
     9601 ggtgacgccg cgcctcgggc gcgccccgtc tgaaagtctc ccgcacgccc tcggcggcgc
     9661 gtcagattct tgaacgagaa gcaacatgga cgcgcccccg cgcgcggcct cggaacccga
     9721 cagccatgag acagaaagag acttcgcaga aaaaagatcg cgcgccggcg gccgcgtcgg
     9781 tggcgacggc cgtgggcggc gcggccggtg cgagcgcgga agagtcgcgg cacgagcggg
     9841 aacgtctcgc ggccttaaac tccgacatcg gcatcgctct cgtgcagggc gacaccctga
     9901 gcgaaaccct ccaccgctgc gccgaagctc tcgtcaagca cctcgacgcg gcgtttgcgc
     9961 gcatctggac gctcaacgag gaggagcagg cgctggagct gcgcgccagc gtcggcctct
    10021 accgacacct cgacggcccg cactcgcgtg tccccgtggg caagttcaag atcggcctca
    10081 tcgccgaaga gcggctgccg cacctgacga actctgtggt cggcgacccg cgcgtcggcg
    10141 atcaggagtg ggccaaacgc gagggcatgg tggcgtttgc cggctatccg ctcatcgtcg
    10201 aggagactct ggtcggcgtg atggcgatgt tcgcgcgcca cgcgctctcg gagaacgacc
    10261 tacaggcgat ggccgccatc gccaacggcg tcgcgctcgg catcaagcgc aagcaggccg
    10321 aaggtgcgct gcgcgagagc gaagaactca atcaggcggt cttgaactcg ctgaccgcgc
    10381 acatcgccgt gctcgacggc gacggccgca tcgtcgccgt caacgacgcg tggcggcgat
    10441 tcgccgagga gaacgagggc gcgggcggcc acgccgttca gggggccaac tacctgcacg
    10501 tctgcacgca ggcggccggc ggcgagccgg acgacggggg ggccgtgctc gccggcatcg
    10561 agtcggtgct caagggcgcg cgcgacagct tcaccatgga atacgcctgc cactcgccga
    10621 cggaagagcg ttggttcctg ctccacgcga accctctgcc gagcgagcgc ggcggcgcgg
    10681 tcgtctcgca catcaacatc accgagcgca agcgaaccga agagacgctc gcccgtctga
    10741 gccgcgagcg cgagacgatg ctcgaagaag tctccacccc gctcgttccc gtgctcgaag
    10801 gcgtgctggt gatgccgctc atcggcacgc tcgacacggc gcggatgcag cgcgccacgc
    10861 aggccgcgct caccgaggtt cggcgcacgg cggcgcgcgc ctgcgtcatc gacatcacgg
    10921 gcgcgcgcat caccgactcg cacgccgtct cgaacctgac gaacctcgtg cagtcgctcc
    10981 gcctcgtcgg cgccgaggcc atcatcaccg gcgtcggggc cgacgccgcg cgctccatcg
    11041 tcggcctcgg cctcgacctc cggggcctgc gcacgcaccg cacgctggcg caggcgctcg
    11101 ccaccttgag gcgtgatgag tgataagtga taagaaagaa aagaagacgg cttttctctt
    11161 tcttatcact tatcactcat cacttatcac taaagacact gcttacacga agaacagcaa
    11221 gggtgaacta ccaaatgctt ccagcaacac tcggcgaact caaacggcag ggctaccgcg
    11281 tgcgccccgt caaggacgag ctgcgcgcca acctcatcga gaagctgcgc gccggcgagc
    11341 ggctgttccc cggcatcgtc ggctacgacg agacggtcgt gccgcagatc acgaacgcgg
    11401 tgctggcgcg tcacaatctg attctcctcg gcctgcgcgg gcaggccaag agccgcatca
    11461 tccgccagct cgtgggcctc ctcgacgagc gcgtcccggc catcgaaggc tccgaggtca
    11521 acgacaaccc cttccgtccc atctccgcct acggccggca attggtcgag cgcgaggggg
    11581 agcgcacgcc catcgtctgg atcgagcgcg aggcccgctt cgtcgaaaaa ctcgccacgc
    11641 ccgacgtgac cattgccgac atcatcggcg acatcgaccc catcaaggcc gcgcgcggcg
    11701 ggcacctcct ctccgacgag ctgaccatcc actacggcct tctgccgcgc gccaaccgcg
    11761 gcctgttcgc catcaacgag ctgcccgacc tcgccggcaa aatccaggtc ggcctcttca
    11821 acatcatgca ggagggcgac gtgcagatta agggctaccc cgtccgtctg cccctcgacg
    11881 tgatgctcat cttctcggcc aaccccgagg acta
//
LOCUS       DASVES010000226        29142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_584258, whole genome shotgun sequence.
ACCESSION   DASVES010000226 DASVES010000000
VERSION     DASVES010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 29142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584258"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..8123)
                     /locus_tag="VF240_09215"
     CDS             complement(<1..8123)
                     /locus_tag="VF240_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7175425.1"
                     /translation="MSQRTKARSASPAESVGDTSTLVELLRRRAGEQPERLAYTFLTD
                     GGTEELRVTYGELDERARSVGAWLQSSGAVGERVLLLYPPGLDYVYAFFGCLYAGAVA
                     VPAYPPRLNHSLRRLQSILDDAGAKFALTTSPILARVPAFAEQAPGLQTARWLATDQL
                     EMTGGAAEAWEHPRVGRETLAFLQYTSGSTSTPKGVMVTHGNVLYNSAYINYGNGHTP
                     QNVSLSWLPHFHDMGLICGIIQPLYSGFHSLLMSPVAFLQRPLRWLEAVTRHGVTHTG
                     GPNFAYDLCVRRIDPEQRGGLDLSSWGMAYNGAEPVRRETLERFAEFFAACGFRREAF
                     SPAYGLAEATLKVSIAAGPNPPVFLDVYAEALAQNRVVEAPAGAPGKRELVGSGQPDF
                     GTQVRIVNPDTAAEVESDSVGEIWVSGPGVAGGYWGRAQETEQTFNARMDKDGETRFL
                     RTGDLGFLKNGELFVTGRLKDLIIINGHNHYPQDIEWTVEQCHPAVLAGCCAAFSVEE
                     RGAERLVVVAEIARPSAGRDADFPNLFDAIRRAVAEQHELEVEKVLLLKKGSIPKTSS
                     GKIQRYACREGFLNATLKVWAEWPAAPHERAQPAASPPELAGKRARPATEPQTHRQDA
                     ADDIQTWLVNKLAETLRVEPREIDAQQPFASFGIGSVQSVSLSGELADWLGRPLPPTL
                     LYDYPTIAALTGFLAGEQQKPRPAEQPDGSAHADSARPTEPIAIIGIGCRFPGAESPE
                     AFWNLLRAGRDAITECSPDPWRTGAGAAGAAHARCGLLPRVEQFDAHFFSISPREALH
                     IDPQQRLLLEVAWEALEAAGQSPARLAGTQTGVFIGISGSDYQLLQQRSSATDGTYFA
                     TGNSHSIAANRLSYLLDLRGPSVAIDTACSSSLVAVHLACQSLLRGESSLALAGGVNL
                     ILNPNLTDGLARAGMLSADGLCKVFDADANGYVRAEGCGVVVLKRLSDALRDGDDIIA
                     QIKATATNQDGRSNGLTAPNGLAQQAVIRRALDGAGVAPSEIDFVETHGSGTPLGDPI
                     EVNSLISVLSEGRAPEQVCALGSVKANIGHLEAAAGVAGLIKTALTLRHAEIPPQIHL
                     KNLNPHLAVGDTPFSVPTRLRPWPESSHRRLAGVSSFGFGGTNAHAVLEEAPALSPAL
                     VENERPLHLLTLSARSESSLKELARRYETFLGEHPEASLPDVCFTANTGRTHFRHRLA
                     LVCDSSDGCLQQLRAFVSGREVEGMYSGRAPARERPDAAGDGADAALLVPEGGDWSAT
                     LSAFARCYVAGVDVDLDKLDENYRRRRVTLPTYPFERRKYWIDEPEDSISGDGMPDIR
                     QPSDGEEDLSKTAPANAGMPEQRAASPIAETAHPLESNEEGRRQEVLQKLCGIVAELL
                     HMRADEVDPHAPFLEMGADSIVLVSAVRTIENSFGIKISIRQLFDDLSTIDALASHIT
                     RHAAPATVNRLELGSEPEPVVTSPKTEALESTWTAAEVARSFERPPAVSESEVERIVS
                     MQLAAFSRLTAQQLESLRYGGAGATRREEEFDNGRVAGRSAALVTESKRDPEANPGLK
                     TSTPYVPYRKITPGANGGLSARQAKHLESLIARYTKRTPQSKRLAQASRHVLADSRAS
                     VGFRLSIKEMLYPITGAHSAGSKIWDVDGNEYVDVTMGFGVHLFGHDAPFIRQALEEQ
                     LKSGIQLGPRSERVGEVAALLCELTGHERVAFCNSGTEAVMTALRLARAATGRTRVAI
                     FSGSYHGHSDGTLAIAEHSDGQLTSLPMAPGVPKKVAEDVVVLEYGTRQSLDYLKLHA
                     TELACVLVEPVQSRNPALQPRAFLEELREITTAAGAALVFDEMVTGFRVHPGGAQGLF
                     GVKADIATYGKIIGGGMPVGVVAGRASFLDGIDGGAWQYADASYPTAETTFFGGTFCM
                     HPLTMAAALATLRHLKHEGPALQQRLNERTSRLVAELNAHFTAHGYPLGMANFGSLFR
                     FVHTANMDLFYYHLMEKGVYIWEWRSGFLSTAHTEEDFRHFERAIKESLAEMREGGFL
                     PERSAPDPANTPHPNGNDEPAPRDAPQELAEGSDGSGWSPLSDAQEQLWLLSEFGEEA
                     SLAYHESVIIELNGTLDIEHMRAAWAATIDRHEALRTAVDGAEGRQRVLAAVDFALPL
                     VDFTGEVSSGREAAVDEWLRREWRRPFDLRRPPLLRVALLKVDESRHLLVLIAHHIII
                     DSWSLSLLVSEVAAVYSAAREQKPRPLPRALQFGEYLRELSRPRYVGQLQQAETYWRE
                     QFSAPAAPLSLANARPPVKTYQGSKVSLRIEAALTDRLKAFSRQRGCTLFMSLFACYA
                     ALLHRLTGQDEVVVGIPVSGRYVEGSEHLVGYCTHLLPVRSRGAQAASFNEYLRSNRN
                     LLLGAYEHQVYTFAKLLALLNPAHDLSRTPIIQTTFNLEPNAAVPEFAGLDAALFSSP
                     VYYTKFDLGVNVVETNGELLIDLDYNTDLFEADSARRIGEHFRTLLEAALTRPDERLA
                     ALPLMTEDERRRLLEELNDTREPLPDVASFHELFEEQARLTPDATAVVCKGTQLTYRE
                     LNCRANQLAHYLKGCGIGSESRVGICVERSAEMLIGLLGILKAGCAYVPLDPEYPRER
                     LLYMLRDAGASVLLTQQRHAGLFPPELTRVLELDTLLPALALESEDDPAPPASADMLA
                     YVIYTSGSTGRPKGVQVTHASLVNLLTSMQRLPGLSANDTLLAVTTISFDIAAL"
     gene            complement(8482..9201)
                     /locus_tag="VF240_09220"
     CDS             complement(8482..9201)
                     /locus_tag="VF240_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175426.1"
                     /translation="MLSPAPEINSLPPFALVLSLEYRPEDAGQVLQLGQQLAASHAEH
                     PRGYRFAAYTEDAGPVQKIHVLAPLQSPHETEERRNVLADTQAQEALANLYEHLKNSE
                     SFIATYIPELSNPFTGGQSGPVPYVYLISAMIKPGLTEEARASAREAGRKIVAAHQQS
                     VKGRNFVTYHSYSGTDDVFHVAVLFAGLGELDEWVENHELLREAYGAEEAARLLDAVG
                     RVSVKYVGRVAKYHPFISNIT"
     gene            complement(9544..10779)
                     /locus_tag="VF240_09225"
     CDS             complement(9544..10779)
                     /locus_tag="VF240_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7175427.1"
                     /translation="MKSLLRLGIVAFFLINSTAAAQPASPSNAPQTQAGAATAPAPQG
                     SAGSGSWYFAVSGDSRDCGNLIMPKIAASVNSLPPQTPASFYWHLGDFRGMQFPDCDM
                     VLPITGSCQKFPPAWGNFAVNYYLNNAWDDFIQNQVKPFGTLPVFLGIGNHELYGHHT
                     REQFRYKFQPWLTQDWIYFQQIADTRRKPPFHAPDLGMTYFHFIRNGVDFIYLDNAGS
                     TQFDAAQLLWLGQVLAADAQDPTVKTIVVGMHAALPNSISSGHAMDHNCQGVCSGSHA
                     YNMLFNASIGPNRKNVYVLASHSHLFQENIFQTPQHQGQVLPGWIVGTAGASQYLMGG
                     AATPSDQFKDCWKTKICYGYLLVEVRPDGTINPQYKLVTEDSPPKGHAGLSDFCFNKN
                     GAVTTQKPPNLNCACGESK"
     gene            complement(11087..12676)
                     /locus_tag="VF240_09230"
     CDS             complement(11087..12676)
                     /locus_tag="VF240_09230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF037824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7175428.1"
                     /translation="MVGGNFYLRPNVLVEPLFNRWYAWSALIAPATAAMYVTNSHLKI
                     MQSFVAAPQVHVAANKNLAMRGGPFINYDADKAPEVKALLEKTTREQSHLFEFARSVQ
                     QLNDILASEAAGGSLEPFYEKIPDNLKGYVELVYDLNNSPSIRFIEGLLYRSQFYSES
                     SQSIALSLCESDERPFALSTPVLPDEHRLHLDIPFRTDALDELFKTKHTPQPFDHVRE
                     LLNLRDGEGGLCRTFFTEEEPRRPERYAGEKVRIRYFGHACVLIETDETSVLIDPLVS
                     YENPGGLPRYTYQDLPERIDHVLITHNHQDHCMFETLLQLRHKIGNVIVPKNNGGGLA
                     DPSLKAVLQNVGFRRVRELDEMETVEIEGGSLTGLPFLGEHADLNIRTKLAHLVRLKG
                     KSVLTAADSNNLEPKLYEHIHEQVGDVDVLFLGMECDGGPLSWLYGPLMSRPLARKFD
                     QTRRLNGSDCERGMSIVDRLRPRQVYVYAMGQEPWLTFLTSIHYTEESRPIIESGRLV
                     AECRGRGIEAERLYGRKEILL"
     gene            complement(12704..13459)
                     /locus_tag="VF240_09235"
     CDS             complement(12704..13459)
                     /locus_tag="VF240_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase II family protein"
                     /protein_id="HEX7175429.1"
                     /translation="MVTLRNTPDWFTTPEPNPQAELRLFCFPYAGGGETIFRRWPSHL
                     PSTIEVCAAQLPGRGRRMKEPPRTRLSTLAREIADAARPYLDKPFALFGHSMGAVIAF
                     ELARLLRRETGTAPLRLFVSGHRAPQIPKSVPSIHNLPEEDFFEELRRFNGTPAEVLE
                     HRELMGLMLPLLRADFELIETYTYTDAEPLDCPITAFGGERDVSVSRAELERWREQTA
                     ANFFLHMFEGDHFFLNEAGPRLLQIIRRDLGTA"
     gene            13707..14612
                     /locus_tag="VF240_09240"
     CDS             13707..14612
                     /locus_tag="VF240_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175430.1"
                     /translation="MSSMSVLLIFVDGLGIGARGPFNPLDRLGDEASPLAVFEDEEPS
                     LPHGGVLVRTDARLGVGGRPQSASGQTTILTGVNAPAALGYHKQGFPNEAMREIIRRH
                     SIFLQLERAGVRPNVFVNAYTRRFFDERPRWVSATTVAVEAAGLPFRTVEDLRAGRAV
                     YHDFTNRLITERGEDVPPRTPEEAAAVLAAVARAHRFTLYEHFLTDRAGHMQDAELAQ
                     RVLSDLARFVRAVLDLVDHGATTVILTSDHGNVEDLSARNHTLNSVPTLVWGPGRERV
                     ARRVRTLADITPTIFEILTEGELAA"
     gene            14609..16036
                     /locus_tag="VF240_09245"
     CDS             14609..16036
                     /locus_tag="VF240_09245"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7175431.1"
                     /translation="MSHETHMAREQQSAEVGPGGEIVNDRREIFGWMMYDWACSVYNT
                     TVITTLLGLYLTEVAQRAVGPRGSIITIGGYRVLTAESLFPYCIGLSVFLQVFLLPVL
                     GAIADFSNVKKRMMAAFCYSAVVATCLLFFVTEKTYLLGGLLLIISNLCFGAAMVFYN
                     AFLPEITTEDMRDRVSSRGFALGYLGGGLLLALNLGMVAGADRLGISGGMAVRLSLLS
                     AGVWWGVFAAYAFRRLRSRAAARPLPAGTSYLTVGFSELRKTFAELRRLPHTLRYLLG
                     YLLYNDGIQTVVTVAGVFIEQELFKSRGLEPDRSFLISLILVIQFTAIGGALLFERVA
                     RLVGTKRAIITALVLWTCVVLYAYGFLQTKAHAWALGAAIAVVMGGSQALSRSLFSQM
                     IPRGRESSFFGIYEISERGTSWMGPVIFALVLDSTGSYRTALLSLVFFFAAGMVVLLF
                     TNTARAIHDAGNLLPEEVKHETAPA"
     gene            complement(16196..16531)
                     /locus_tag="VF240_09250"
     CDS             complement(16196..16531)
                     /locus_tag="VF240_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175432.1"
                     /translation="MKLLFIFLLMLALVGVYAYWRLRPYIRAVRQIFGVARGATVRVG
                     QRTQGAGDAAQVGDQSAAESLVRCASCGTWIPASRAVSLGGRSAQFCSHTCLEDAANA
                     PATTRRSAS"
     gene            complement(16701..16967)
                     /locus_tag="VF240_09255"
     CDS             complement(16701..16967)
                     /locus_tag="VF240_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175433.1"
                     /translation="MANRKGVFIGAYVPSDLKESLRKRAAAEHRTLSQEITRILEEAV
                     NGPRLPPGFVDRRIPGSPSRRRASDPLPRRRKDDLPYSANSEEE"
     gene            complement(17052..17520)
                     /gene="rnpB"
                     /locus_tag="VF240_09260"
     ncRNA           complement(17052..17520)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF240_09260"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17650..18429)
                     /locus_tag="VF240_09265"
     CDS             complement(17650..18429)
                     /locus_tag="VF240_09265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7175434.1"
                     /translation="MTDDHARESGGGLEDKSTFSPPPLSRPAVRKTHLSESADKAAAN
                     DRALAAWEIVSVTLSVLIAEWVVLALADSGSSLVNFAVGLFAFTFMLLSKSVRKETAR
                     DLGWRFDNFPRAMRLLALPTLAMTTFLLLAGALAGSIDFSRWRGGWAFVGVPTFVLAW
                     ALVQQYALQAFINRRAQIICGRGPRSVVLVSAVFALAHFPNPALTAVTFAGGLVWAAV
                     YQRAPNLPALAVSHALMTWVLVSTLPPQLLGGLRVGYKYFG"
     gene            complement(18426..20786)
                     /locus_tag="VF240_09270"
     CDS             complement(18426..20786)
                     /locus_tag="VF240_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175435.1"
                     /translation="MKRNKISAAALAALLVFAPFANVAGVRAAAGTAAGAQDDGPSDA
                     EQSMFTRGQNLFIQGNYEQAISVLRDFLKTYPNSVISDLTLLWLGRSYMQTGRIQDAE
                     QVGQRLRTIRETPFVDIYESELKTAREEIARRPPVTQPGAPATRPRIDSTPLPAVAAA
                     ATPTPERPAAREAAAVSQPSTPGTTAVTDTRTPRLVPRSEGTSERSRRAGTRRGSNIP
                     ADTAAAAVQPTPRATPTPTPSRRRDTREVARVNTTPPPVTAPSVEPARVPETSDPAAT
                     LRTSTAPADSSSPQSSSFVITVRQVPNLVLALRRAAISASPGQTVQLPLVVTNTGNKD
                     DQFSIETDLPAEFQPTFSAAQGGAGTEVPIMVTPKLPRGANYDVLLNLRIPDAAPDNK
                     QLSFFVRAASQTDHQVFKVADAALTVAAAALIATTDLSQESVMPGETFTQNITMKNQG
                     SAPARGARVDFVFNPDFELVSANPSPLVYDRPSRTAIWNLGEVDSRQGRDISVTLKAV
                     PEALAATRSVGRGTMKTQSLIIPANFDGPSITVGRVARARIDSVTTGLTATPGDTIYL
                     PFIVRNPGNYPESFEVRVTAPGAPTATVYADTNGDGLHQEGEPSVTQTAQIEPRGGQY
                     PILLRVDIPRSTPDRQQYAYNVVTRALAAGRVASEASTVLTVATPRVRVRPELPTSDV
                     KPGDTIFYRLVLVNDGAGLAKNLVLTEQLPDALEFISSEPSLNSQDAPGSAQRFVWRV
                     TELSPGDTAVLKIAVRIKMNIEADRTLSPSHSLTFQDGNGNTYRGQ"
     gene            complement(20970..22208)
                     /locus_tag="VF240_09275"
     CDS             complement(20970..22208)
                     /locus_tag="VF240_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7175436.1"
                     /translation="MKKVLLGVIIAAFAADVSAARPQTPSVRVGPAADESAPNDSAVA
                     KKDDERATDKSGNSAKIDPKKNAKNSKTGDAGKSATERASNATPNPAAATAAPQMTSD
                     ATGATSERTTPPVNSPKVTPAPDKGSPAAGGTASPAGATSAAPGSASFSNINAAAPPH
                     APAPTSIYRVGVGDVLDIRLQNVGPRESTLFAVLAGGLIDYPLAGDPFNVAGMTTDEV
                     GVRVAAELRRRAVYDKPQVYVSVREYGSHTVLVSGLVYDPGVKILRREAIPLYVLIAE
                     AQPKPEAGRALVMSHSTGQSKTLDLSDTQGLNTLIHPSDVVNLVARPQEFFYIGGEIS
                     APGQKNYHPDMTLTQALLASGGVTRTSSGAVRVSRQSADGRLVPATYDLRLIQEGKIP
                     DPALQPGDRIEVEAAGKKKN"
     gene            complement(22483..24336)
                     /gene="ptsP"
                     /locus_tag="VF240_09280"
     CDS             complement(22483..24336)
                     /gene="ptsP"
                     /locus_tag="VF240_09280"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX7175437.1"
                     /translation="MSRAGAVGKAEELSRRERKERAEERLRGVAVSDGVGAGRVLRIH
                     SGAREHVYKASLEETEVAREARRFRAAVRLARRQLQVVKRRAEKALGEGHAYIFDAHL
                     LMLEDRRLLEEIEEHIRGGRVNAEWAVKVVTDRLLAVYAEIKDDYLRERSSDIEDVTR
                     RLLVALSGESMPARRVMEDAVIVAEELLPSAAAELDFERVRAIATDGGGWTSHTAIIA
                     RGMGIPAIVGLRDLYRRARTGDEIVVDARKGEVVLHPSGVTSGRYRTDESAEPATRGG
                     VAAAGGNGGRGEPLLTLDGVDIILRANVELPAEFEGVSRYGARGVGLYRSEFLMTQRG
                     APPGEEEQYRAYLEVAELGGADGACVRLFDLGGEKLSADFDGSGEHNPALGLRAIRYS
                     LRNLDVLRTQARAVLRAAARGPLDLVLPMISDVTDVRRARAVIEEEGARLAREGCEAG
                     KVRVGAMIEVPSAVLVAESLAREVDFFSLGTNDLVQYLLAVDRGNDEVAEWFRSLHPA
                     VLQSIRRTLRAAEGAAIPAVICGEMAATPAYAVVLVGLGARQLSMTPSSIPRVRRALA
                     GVEAERAAGVAKECEECETADDAEEVVRVRLGELWPNLFPPETLPPSKSGM"
     gene            complement(24333..24626)
                     /locus_tag="VF240_09285"
     CDS             complement(24333..24626)
                     /locus_tag="VF240_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX7175438.1"
                     /translation="MVECSVRVVNRLGLHARAAARLVRTACTFRCRIRVERADRSASA
                     DAKSILSVLMLAASRGTELRVSAEGDDEQEAVEAVCALIADGFGETEEEPRPA"
     gene            complement(24630..25091)
                     /locus_tag="VF240_09290"
     CDS             complement(24630..25091)
                     /locus_tag="VF240_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7175439.1"
                     /translation="MSDNSEKREGERAAPPGRVAGVLVTHGRLGSELLTAAETIIGTI
                     SHVAAVSIGWHDDVDAASDEIERAVTRVSEGRGVLLLTDLFGGTPTNIASMFLKEGEA
                     EVVTGVNLPMVIKLASQSGDESLADVARRVRDQGREGIYLAGELLAGAKKG"
     gene            complement(25348..26307)
                     /gene="rapZ"
                     /locus_tag="VF240_09295"
     CDS             complement(25348..26307)
                     /gene="rapZ"
                     /locus_tag="VF240_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX7175440.1"
                     /translation="MTEKRRKRKAGGGGAVNNGAGDGAPDIVIITGLSGSGMSSATNA
                     FEDLGYFCVDNLPLTLLPTFARLVQEDDEERPHIGRAALAINIREGQFLSEFPGRLAA
                     LRRSGLKVFVIFLEASDDALHRRFSETRRPHPFDTGDGLASSIRSERQAMDEIRGLAD
                     HVIDTSEHTVHSLRRLLVERFSPDAEGTPMRVQVLSFGHKYGAPRELDLLVDVRHLPN
                     PHFVPELRHLSGHSGEVVSYLNASEEVGETLRRFGDLLDYLLPLYRREGKSYVTVGVG
                     CTGGRHRSVMIANALARRLRRAGFAAAATHRDVKKTRKAKRQQ"
     gene            complement(26307..27299)
                     /gene="hprK"
                     /locus_tag="VF240_09300"
     CDS             complement(26307..27299)
                     /gene="hprK"
                     /locus_tag="VF240_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX7175441.1"
                     /translation="MTEHPSVTVAEFVADAPAQLGLRVINGAAGAGTRRLDAARIQKL
                     GLALAGFTHYIHPGRIQLIGQSEVQFLSQLTPEGRREAVGNLELEKISCILITKGLLP
                     PSELSEVAERAALPIVQSPLITSTAIAVITEYLQEVLAPRERRHGVLLEIHGLGVLIE
                     GESGIGKSECALDLLMRGHRLVADDVVEVWRAGPGTLRGSAPELLREHMEIRGLGILN
                     VRELFGVTATSGPVVVGLSISLERWHDAGEVERLGLDAARVEILGVEVPRVIIPVSPG
                     RNLSTLVEAAVRVHLLRVRGYNAAREFVARHSAAIGDGDKQEGRGADTGAEHPG"
     gene            complement(27390..27989)
                     /locus_tag="VF240_09305"
     CDS             complement(27390..27989)
                     /locus_tag="VF240_09305"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7175442.1"
                     /translation="MLKRSAFIVIVALLLVVSPVLGATTKYEIDTAHSNVGFSIPIMG
                     GLSQVRGKFTDFAVTIVYDDKDVTKSSVTAVIKAASIDTGIERRDAHLRNPDFFDVEK
                     FPEITFKSSRIEKKGKNFIAHGAFTMHGVAKEIALPFTVNGVTKDEKTGKTTLGARAA
                     VTLNRRDFGINFARPDNPTFLGDTVEVELNLITRAASPQ"
     gene            complement(28122..28679)
                     /gene="raiA"
                     /locus_tag="VF240_09310"
     CDS             complement(28122..28679)
                     /gene="raiA"
                     /locus_tag="VF240_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7175443.1"
                     /translation="MRFEYTGRHVEVTPAIRRHVEEHFGMIGHLFEGSSTVSTHVIIE
                     VEKNRQNAEILLHWRDHTLTAKDTNADMYQALTRCIDKIEKQAVRLKKKIIGRRQSAT
                     PLSALEPTPATEQLAAETAEAAKAAGPRIINARRYRVKPMTAEEAVLALSDEDNQFLV
                     FRDADTDSLSVIYKRKDGNFGLIQP"
     gene            complement(28892..>29142)
                     /locus_tag="VF240_09315"
     CDS             complement(28892..>29142)
                     /locus_tag="VF240_09315"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175444.1"
                     /translation="VIELRRFFTEGMMNEDGEEVSTRIIKLRIKKLIEEEDSKNPITD
                     DQIVKILIGEGIKLSRRTVAKYRDQMSIPGSRERKAVV"
BASE COUNT         4880 a         9142 c         9619 g         5501 t
ORIGIN      
        1 agcgcggcga tgtcgaagga gatggtcgtc acggcgagca gcgtgtcgtt cgcgctcaga
       61 ccggggaggc gctgcatcga ggtgagcagg ttgacgaggg aggcgtgggt gacctgcacg
      121 cccttgggcc ggcccgtcga accggaagtg taaatcacgt aggccagcat gtcggcggac
      181 gcgggcggag ctgggtcatc ctcactctcc agcgcgaggg caggcagaag cgtgtcgagt
      241 tcgagcacgc gggtcagctc cggcgggaac agaccggcgt gccgttgctg cgtaagcaac
      301 acggaagcgc cggcgtcgcg caacatgtat aacagccgct cgcgcgggta ttccgggtcg
      361 agcggcacgt aagcacaccc ggccttcaga attccaagca gccctatcaa catctccgcg
      421 gagcgctcga cgcagatgcc gacgcgcgac tcagacccga tgccgcaccc cttcaggtag
      481 tgcgccaact gattggcgcg gcagttcaat tcccgatacg tgagttgcgt gcccttgcac
      541 acgacggccg tcgcatccgg cgtgagccgc gcttgttcct cgaacaactc gtggaaggac
      601 gccacgtcgg gcagaggctc ccgcgtatcg ttcaactctt cgagcagcct gcggcgctcg
      661 tcttcggtca tgaggggcag cgccgcgagc cgctcatcgg gccgcgtcag cgcggcttcg
      721 agcaacgtgc ggaaatgttc tccgatgcgg cgcgcggagt ccgcttcgaa caagtccgtg
      781 ttgtagtcga gatcgatcag gagttcgccg ttcgtctcga cgacgttcac gccgaggtcg
      841 aacttcgtgt agtagacggg cgaagagaac agcgccgcgt ccagcccggc aaattcgggg
      901 acggcggcgt tcggttcgag gttgaatgtc gtctggataa tcggggtgcg gctcaggtcg
      961 tgcgccgggt tgagcagggc taagagtttc gcgaacgtat acacctgatg ctcgtaagcg
     1021 cccagcagga gattcctgtt cgagcgcagg tactcattaa agctcgctgc ctgtgcgccg
     1081 cgactgcgga caggcagcag gtgcgtgcag tagccgacga ggtgctcgct cccttcgaca
     1141 taacgccctg acactggtat cccgaccacg acctcgtcct gtcccgtcag cctgtggagg
     1201 agcgccgcgt agcaggcgaa caacgacatg aacagggtgc agcctctttg cctgctgaac
     1261 gccttgaggc ggtcggtgag agcggcctcg atgcgtagac ttactttcga gccttggtac
     1321 gtcttgaccg ggggccgcgc gttcgccagt gagagcgggg cggcgggcgc ggagaactgc
     1381 tctcgccagt aagtctccgc ctgctgcaac tgcccgacgt aacggggccg cgacaactcg
     1441 cgcaggtact cgccgaactg caacgcgcgc ggcagcgggc gcggcttctg ttcgcgcgcc
     1501 gccgagtaga cggccgcaac ttcgctgacc agcaggctga gcgaccaact atcgatgatg
     1561 atgtggtggg caatcaggac gagcaggtgc ctgctctcat ctaccttgag aagcgcgacg
     1621 cgcagtagcg gcggtcgccg caggtcgaac ggccgccgcc actcacgcct cagccactcg
     1681 tcaaccgcgg cctccctccc cgaacttacc tcccccgtga aatcaacgag cggcaatgcg
     1741 aaatcgacgg cggctaaaac tctctgacga ccttccgcgc cgtccacggc cgtcctcagc
     1801 gcctcgtgtc tgtcgatggt cgccgcccac gcggccctca tgtgctcgat gtcgagagtg
     1861 ccgttcagct cgatgatgac ggactcgtga taggcgagcg atgcttcttc cccaaactct
     1921 gacagcagcc agagctgctc ctgtgcatcg gacagtggcg accagcccga gccgtcagac
     1981 ccttcggcca actcctgagg cgcgtcgcgc ggggccggct cgtcgttgcc gttcgggtga
     2041 ggagtgttcg caggatcggg cgcgcttcgt tcggggagga acccgccctc tcgcatttcc
     2101 gcgaggctct ctttgatggc ccgttcaaag tgtcggaagt cttcctcggt gtgcgcggtg
     2161 gagaggaagc cgctccgcca ctcccagatg tacacgcctt tctccatcag gtggtaatag
     2221 aacaagtcca tgttggcggt gtggacaaaa cgaaagagcg agccgaagtt cgccatcccg
     2281 agagggtagc cgtgcgcggt aaagtgcgcg ttgagttccg caaccaaacg cgaggttcgt
     2341 tcattcaaac gctgctggag ggcggggcct tcgtgcttga ggtgtctgag cgtcgcgagg
     2401 gccgcagcca tcgttagcgg gtgcatgcag aaggtgccgc cgaagaaggt ggtctcggct
     2461 gtcggatacg aggcgtcggc atactgccat gccccgccgt cgatgccgtc cagaaaggat
     2521 gcccgtccgg ccacgacccc gacgggcatc ccgccgccga tgatctttcc gtaggtcgcg
     2581 atgtcggcct tcaccccgaa caagccctgc gcgccgccgg ggtgaacgcg aaaacctgtg
     2641 accatctcgt cgaagacgag cgccgcgccc gcggccgtgg ttatctctct caactcctca
     2701 aggaaggcgc gcggctgcaa agccgggttg cgactctgga cgggttcgac cagcacgcac
     2761 gccagctccg tagcgtggag tttgagatag tcgagcgatt gccgtgttcc gtattcgaga
     2821 acgacgacat cttcggcgac cttcttcggc acgccggggg ccatcgggag cgaagtcagt
     2881 tggccgtcgc tgtgttccgc aatggccaac gtcccgtcgc tgtggccgtg ataagaaccg
     2941 gagaagatgg ccacgcgtgt gcgcccggtc gcagcccgcg ccagacgcag cgccgtcatc
     3001 acggcctcgg tgcccgagtt gcagaacgca actctctcgt ggcccgtcaa ctcgcacagc
     3061 aacgccgcca cctcgccgac gcgttcggag cgcgggccga gttggatgcc ggacttcaac
     3121 tgctcttcga gcgcctgccg gatgaacggg gcgtcgtggc cgaacagatg aacgccgaag
     3181 cccatcgtca cgtccacgta ttcgttgccg tccacgtccc aaatctttga gccggcggaa
     3241 tgtgcccccg taatcgggta gagcatttcc ttgatggaca gacggaagcc gacggaggcg
     3301 cggctgtcgg cgaggacgtg tcgggaggct tgcgccagtc gcttggattg gggcgtgcgc
     3361 ttcgtgtacc gggcgattaa agattccagg tgctttgcct gacgcgcact cagcccgccg
     3421 ttcgcgcccg gtgtgatttt ccggtagggg acataaggcg tagacgtttt gaggccgggg
     3481 tttgcttcgg ggtctctttt tgattccgtc acgagggcgg ccgagcgacc ggccacgcgg
     3541 ccgttgtcaa attcttcttc tcgcctcgtc gctccggccc cgccgtatcg tagagactcc
     3601 aactgttgcg cagtcagacg tgagaacgcc gcgagctgca tactcacgat acgctccacc
     3661 tcggactccg agactgccgg aggtcgctcg aacgaccttg cgacctcggc ggcagtccac
     3721 gtggactcta acgcttccgt cttcggagaa gtcacgacgg gttccggctc gctgcccaat
     3781 tcaagtctgt tgacggtcgc cggggccgcg tgtcgcgtga tgtgcgaagc cagcgcgtcg
     3841 atagtcgaga ggtcgtcgaa aagctggcgg atggatattt ttattccgaa gctgttttcg
     3901 atggtgcgga cggcgcttac cagaactatc gaatcagcac ccatctcaag aaatggcgcg
     3961 tgggggtcaa cttcgtccgc ccgcatgtga agcagctcgg cgacgatgcc gcatagcttt
     4021 tgtagaactt cctgcctgcg accctcctcg ttgctttcga gtggatgcgc cgtctccgcg
     4081 atgggactcg cggcccgctg ttcaggcatc ccggcattgg cgggcgctgt ctttgataaa
     4141 tcctcttccc cgtccgaggg ttgtcgaatg tcaggcatac cgtctccaga gatcgaatct
     4201 tcaggctcgt ctatccagta cttccggcgc tcgaaagggt aggtgggtag agtgacgcgg
     4261 cgacgccggt agttttcgtc aagcttgtcc aagtcaacat cgactcccgc aacgtagcac
     4321 cgggcaaagg ccgaaagcgt cgccgaccag tctccacctt cgggcacgag gagtgccgcg
     4381 tccgcgccgt cacctgcggc gtcagggcgc tcccgtgccg gcgcccgccc gctatacatt
     4441 ccctcaacct cacgcccgct cacgaaagca cgtagctgtt gcaggcaacc gtcggaagag
     4501 tcgcagacga gcgcgaggcg gtgtcgaaaa tgagtcctgc cggtattcgc cgtaaagcaa
     4561 acatcaggta acgacgcctc tggatgttca ccgagaaagg tctcgtaccg gcgggccagc
     4621 tccttcagcg aactctcgct gcgcgccgag agagtcagca gatgtaaagg gcgttcgttc
     4681 tcgacgagtg cggggctgag cgccggcgct tcctccaaca cggcgtgcgc gttcgtcccg
     4741 ccgaagccga aggaactcac ccccgcgagc cttcggtgtg acgactcggg ccagggccga
     4801 agccgcgtcg gcacggagaa aggcgtgtcg ccgacggcga ggtgcgggtt taagtttttg
     4861 agatgtatct gcggcggtat ctcagcgtgc cgcagcgtga gcgcggtttt tatcaacccc
     4921 gccaccccgg cggcggcctc cagatggccg atgttggctt tgaccgagcc gagggcgcag
     4981 acttgttcgg gcgcacgccc ttcgcttaat accgagatta aggagttgac ctcgatgggg
     5041 tcgccgagcg gcgtccccga cccgtgcgtc tcgacgaaat caatctccga cggcgcgacc
     5101 cccgcgccat ccagtgcgcg gcggatcacc gcctgttgcg ccagcccgtt cggcgcggtc
     5161 agcccgttgc tgcggccgtc ctgattggtt gccgtggctt tgatctgggc gatgatgtcg
     5221 tcgccatcgc gcagcgcgtc ggacagtcgt ttgagaacga cgactccgca accctcggcg
     5281 cgcacgtatc cgttggcgtc ggcgtcgaag actttgcaaa ggccgtccgc cgagagcatc
     5341 cccgcgcgcg cgaggccgtc agtcagattc ggattcagga tgagattgac gccgcccgca
     5401 agtgcgaggc tcgattcacc gcgcagcagg ctctggcacg ccagatggac ggcgacaagc
     5461 gaagacgagc acgccgtatc aatcgcgacg ctcgggccgc gaaggtctaa aagatatgag
     5521 agacggttgg ccgcgatgct gtgggagtta cccgtggcga agtaagtccc gtcggtggcg
     5581 gagctgcgct gctggagcag ttgatagtcg ctgccgctga taccgatgaa gacgcccgtc
     5641 tgagttcccg cgagtcgtgc gggcgactgt ccggccgctt cgagagcttc ccacgccacc
     5701 tccaaaagca atctctgttg cgggtcgatg tggagcgctt cgcgcggtga gatggaaaag
     5761 aagtgcgcgt cgaactgttc cacccgaggg agtagcccgc accgagcgtg tgccgctccc
     5821 gccgccccgg ctcccgtgcg ccaggggtcg ggagagcact ccgtgatcgc gtcgcggccc
     5881 gcgcgtaata gattccagaa cgcttcggga ctctcagcgc cggggaagcg acagccgatg
     5941 ccgatgatgg cgattggctc ggtcggccgc gcggagtcgg cgtgcgcgga accgtcaggt
     6001 tgctccgcag ggcggggctt ttgctgctcc ccggccagaa agcctgtgag cgccgcgatg
     6061 gtggggtaat cgtagagaag cgtggggggc agcgggcggc cgagccagtc ggcgagttca
     6121 cccgagaggc tgacgctctg cacggagccg atgccgaaac tggcgaacgg ttgttgggca
     6181 tcaatctccc gcggctcgac gcgcagtgtc tcggcaagtt tgttgaccag ccacgtttga
     6241 atatcgtcgg ccgcatcctg tctgtgagtc tgtggctccg tcgccggacg cgcacgtttc
     6301 cctgcaagct ctggcggcga cgcggcgggt tgcgcgcgtt cgtgcggcgc cgccggccat
     6361 tccgcccata ccttcagcgt ggcgtttaaa aatccttcgc gacaggcgta gcgttgaatc
     6421 ttcccgctgg aggtcttggg gatactccct tttttgagca gcagcacttt ttccacttcc
     6481 agttcatgct gctcagccac ggcacgccga atcgcgtcga agagattggg gaagtccgca
     6541 tcacgccccg ccgacgggcg cgcgatctcg gcgacgacga cgaggcgttc ggccccacgc
     6601 tcctccacgg aaaatgccgc gcaacaccct gcgagcacgg ccgggtgaca ctgttcgacc
     6661 gtccattcga tatcctgcgg gtagtgattg tggccgttga taatgatgag gtctttcaga
     6721 cggcccgtga cgaacagctc accgtttttt agaaagccca aatcgccggt tcgcagaaag
     6781 cgagtctcgc cgtctttatc catgcgggcg ttgaaggtct gttccgtctc ctgtgcgcgc
     6841 ccccaatacc ctcctgccac gcccggcccc gaaacccaga tttcaccaac actatctgat
     6901 tcaacttcgg cggccgtgtc ggggttgacg atccgaacct gcgtcccgaa gtcgggctgt
     6961 cccgagccga ccagctctcg ctttccgggc gcgcccgccg gggcttcgac cacgcgattt
     7021 tgcgccagcg cctccgcgta cacgtcgaga aagacgggcg ggtttggccc cgcagcgata
     7081 gacactttca gggtcgcttc cgccagaccg taggccgggc tgaatgcctc gcggcgaaag
     7141 ccgcacgcgg cgaaaaactc ggcgaagcgt tcgagcgtct cacgtcgaac cggctcggcg
     7201 ccgttgtagg ccatacccca actgctcagg tcgagcccgc cgcgttgttc cgggtcgatg
     7261 cggcggacgc agaggtcgta agcgaagttt ggcccgcccg tgtgcgtcac gccgtggcgc
     7321 gtgacggctt cgagccatcg cagggggcgt tgcaggaaag cgacgggcga catgaggagg
     7381 ctgtggaagc cgctgtagag cggttgaatg atgccgcaga tcagccccat gtcgtggaag
     7441 tgtgggagcc acgaaagaga gacgttctgc ggcgtgtggc cgttcccgta gttgatgtat
     7501 gcggagttgt aaaggacgtt gccgtgagtg accatcacgc ctttcggcgt ggaggtcgag
     7561 cccgaagtgt attgcaggaa ggcgagcgtc tcgcgcccca cacgcgggtg ctcccacgcc
     7621 tcggccgctc cgcccgtcat ttcgagctgg tcggtcgcca gccaccgcgc cgtttgtaga
     7681 ccgggggcct gctcggcgaa ggcgggcacg cgggcgagaa tgggcgaggt cgtgagggcg
     7741 aatttggcgc ccgcgtcgtc gaggatggac tgtagacgac gcagcgaatg gttcaagcga
     7801 ggcgggtagg ccgggaccgc gacggcgccc gcatacagac aaccgaagaa agcgtagacg
     7861 taatccagac ccggcgggta gagcagaagc acacgctcgc cgaccgcgcc ggaagactga
     7921 agccacgcgc cgacggagcg cgcccgctcg tccaactcgc cgtaggtgac acgcagctct
     7981 tccgtcccgc cgtcagtgag gaaggtgtaa gcaagccgct cgggctgttc gccggcccga
     8041 cgacgcagaa gctcgactaa tgtggatgta tcgccgaccg actctgcggg cgaggcggag
     8101 cgggctttcg tcctttggct cacaatgctc tcgacgggtg tgttcggcgc gcgccctgaa
     8161 ggctcggccg aacgtctttt tgtctgtccg acgccacgct gtgtcgttgg caggtgggtt
     8221 ctaacgctgc gacgattgcg acgacacctt ccgggacgag gccgcccggc aaatgcctgt
     8281 gcagccgggt gttcaccaac gtgaggccac cacctcttaa acttgattta cgtggtgaag
     8341 gtctgacggt gacttacctg cttcgatctg cgcggttcaa tggggacact acggacactt
     8401 agcaacagca atgaggctat acgtgtattg tcgggttccc gcgatgctgc tcttgaagag
     8461 gaatcttgat cggcttgttc gtcaggtaat attactgata aatggatgat acttcgccac
     8521 gcgtcccaca tatttcacgc tcacgcggcc gacggcgtcc agcagtctcg ccgcctcttc
     8581 cgcgccatac gcctcccgta gcaactcatg attttcgacc cattcgtcca actcgccaag
     8641 gccggcgaac aggacggcca catgaaagac atcatccgtc ccgctgtatg agtggtacgt
     8701 gacgaagttg cgccccttca cgctctgctg gtgcgcggcc acaattttcc tgccggcctc
     8761 gcgggccgaa gctcgcgcct cctccgtcaa gccgggttta atcatggccg agatcagata
     8821 aacgtatggg acaggcccgc tctgccctcc ggtgaaggga ttgctcagtt cagggatata
     8881 ggtggcgatg aaactttcgc tgttcttcag gtgctcgtag aggttggcga gggcttcctg
     8941 cgcctgtgtg tcggcgagca cgtttcttct ttcctcggtt tcgtgtggag actgaagagg
     9001 ggccagcacg tggatttttt gcaccgggcc ggcatcctcg gtgtaagcgg cgaagcggta
     9061 gccgcgggga tgttcggcgt gggaggcagc gagctgctga cccagttgca agacctgtcc
     9121 cgcgtcctcc ggccggtatt ccagactcag caccagagca aatggaggca aactgtttat
     9181 ttctggagcg ggtgagagca cggagaaaat cctttctcct cacaagccgg tgagtgcttt
     9241 gtcgagaggc aaaatttcgc cgggttagag gacttgcaac aatctttgca gcgagagtta
     9301 tatgtcacta tttaacagtt tgtcaatatt ctcgcccctg aacatctgtg tgttcagggc
     9361 gggggcacgg atgcctccgg ggccgggttt ccggggcgcg cgatccgtgt tttgaagggc
     9421 ctggggcggc aggggtcggg gcttgatggg atgagcgccc gcgcggccaa cagagtcgcg
     9481 tggcgcgagg ttgagccgtg cgtctttacg ggacttgccg gttggcgcct gcgggccgtt
     9541 ttgctacttc gattcgccgc aggcgcagtt caaattcggg ggcttctgcg tggttacggc
     9601 tccgttctta ttgaagcaga agtcgctcaa gccggcgtgt cccttcgggg gcgaatcctc
     9661 cgtgaccagc ttgtattgcg ggttgatagt cccgtcggga cgcacctcca ccagaaggta
     9721 gccataacaa atcttggtct tccaacaatc cttgaactga tcggaggggg tggcggcccc
     9781 gcccatcaag tattgcgaag ctccggcagt cccgacaatc caaccgggca gaacctgacc
     9841 ctgatgttgc ggcgtctgga agatgttttc ctgaaagagg tgtgagtggc tcgccagcac
     9901 gtagacgttt ttccggttcg gcccgatgga agcgttaaag agcatgttgt aggcgtggct
     9961 gccggaacaa accccctggc aattatggtc catcgcatgg ccactggaga tgctgttggg
    10021 cagggctgcg tgcatcccga cgacgatggt cttgacggtc gggtcctgcg cgtcggcggc
    10081 cagcacctga cccagccaga gaagttgggc ggcgtcgaat tgcgtcgagc cggcgttatc
    10141 gaggtagatg aaatcgacgc cgttcctgat gaagtgaaaa taggtcatcc cgaggtctgg
    10201 cgcatggaac ggaggtttcc tccgcgtgtc cgctatctgc tggaagtata tccagtcctg
    10261 cgtcagccac ggctggaact tgtaccggaa ttgttcgcgc gtgtgatggc cgtacaactc
    10321 gtgattcccg atgccgagaa agacgggcag agtgccgaat ggcttcacct gattttgaat
    10381 aaaatcatcc caggcgttgt tcagataata attcacagcg aagttgcccc aggccggggg
    10441 gaacttctgg caactgcccg tgatgggtaa gaccatgtcg cagtccggga attgcatccc
    10501 ccggaagtcg ccgaggtgcc agtagaacga ggccggtgtc tgcgggggga gactattaac
    10561 ggaggcggca atcttgggca tgatgaggtt gccgcagtcg cgcgaatcgc cggagacggc
    10621 gaagtaccaa ctgccgctgc cggcgctgcc ctgtggggcc ggggcagtcg ccgcgccggc
    10681 ttgagtttga ggagcgtttg aaggactggc tggctgggcc gcagccgttg agttgattaa
    10741 gaagaaagca acgatgccga gacggagaag agatttcatg ggggcgctcc tttaaactgg
    10801 ggcgggtttt gatggccgcc ttcctgctca acgaaagacc gctagcctcg acctacctgc
    10861 atactgctgg cgcaagcccc tcgccacggc ggcttcccct ccctttgacc gcctccgaaa
    10921 tgtactggta ttgccgtggt gagactgtcg ggcaatgatc gtgctattgg gtaatcagag
    10981 ttgaagcgcg gtaacaatat gaccgttccg cgtcggtgtc aatacttatg gcggccgggg
    11041 ccggggcccc tttaacgctc gacacaaacc gtttcggcgc gcgtactcac agaaggattt
    11101 ccttgcggcc gtacaggcgt tccgcctcaa tccccctccc ccgacactcc gcgaccagtc
    11161 ggcccgactc gatgattggg cgcgactctt ccgtgtagtg aatcgaggtg aggaaggtca
    11221 accacggctc ctgacccatc gcgtagacgt agacctgtcg ggggcgcagt ctatcgacga
    11281 tgctcatgcc gcgctcgcaa tcggagccgt tgagccttcg cgtctggtcg aacttccgcg
    11341 ccagtggccg ggacatgagc gggccgtaga gccacgagag ggggccgccg tcgcactcca
    11401 tcccgagaaa gagcacgtca acgtcgccga cctgttcgtg gatgtgttcg tagagtttcg
    11461 gctcaaggtt attcgagtcg gcggcggtga ggacggattt gcctttcagc cgcacgaggt
    11521 gggcaagttt cgtcctgatg ttcaagtcgg cgtgctcgcc gaggaaaggc agacccgtca
    11581 gcgacccgcc ctcgatctcg acggtctcca tctcgtcgag ttcccgcacg cgccggaagc
    11641 cgacgttttg caagacagct ttgagtgagg ggtcggccag cccgccgccg ttgttcttcg
    11701 gcacgatgac gttcccgatt ttatggcgga gttgcaggag cgtctcgaac atgcagtgat
    11761 cctgatggtt gtgcgtgatg aggacgtggt cgatccgttc gggcaaatcc tgatacgtgt
    11821 agcgcggcag accacccggg ttctcgtagc tgaccagcgg gtcgatcagg acgctcgtct
    11881 cgtccgtctc gatcagaaca catgcgtgtc cgaagtagcg aatccttacc ttttcgcctg
    11941 cgtacctctc gggccggcgc ggctcctcct cggtgaagaa agtgcggcac aagccgccct
    12001 cgccgtccct gaggttcaat aactcacgga cgtggtcgaa aggctgcggc gtgtgcttcg
    12061 tcttgaacaa ctcgtcgagc gcgtcggtgc ggaacgggat gtcgagatgc agtcgatgtt
    12121 cgtcaggcag gacgggtgtg ctgagggcga atgggcgctc atcactctcg cacagcgaga
    12181 gcgcgatgct ttgcgacgac tcgctgtaga actgactccg gtagagcagc ccctcgatga
    12241 agcgaatcga aggactgttg ttcaagtcgt aaacgagttc cacgtagcct ttcaggttgt
    12301 cgggaatttt ctcgtagaaa ggttcgagtg aaccgcccgc tgcttcactt gcgagaatgt
    12361 cgttgagctg ctgaaccgat ctggcaaact cgaagaggtg cgattgctcg cgcgtcgtct
    12421 tctccagcag ggccttgacc tccggcgcct tgtccgcgtc gtaattgatg aatggcccgc
    12481 ccctcatcgc gaggtttttg ttggcggcga cgtgcacttg cggggccgcg acgaacgact
    12541 gcatgatctt caggtgtgaa ttcgtcacgt acatcgcggc ggtggcgggg gcgatgagcg
    12601 ccgaccacgc ataccaccga ttgaacaacg gttcgacgag cacgttcggc cttaagtaaa
    12661 aattcccacc gaccatttga cgttgtcctt tcctttggat cgatcatgcc gttccgaggt
    12721 cacgccggat gatttgcagc agtcgcggcc cggcttcgtt gaggaagaag tgatcgccct
    12781 cgaacatgtg caggaagaaa ttggccgcag tttgttcgcg ccaacgttca agttctgcgc
    12841 ggcttacgct cacgtcacgc tctccgccga aggccgtgat cggacagtcg agcggctcag
    12901 cgtccgtgta agtgtatgtc tcgatcaact cgaaatccgc acgcagcagc ggcagcatca
    12961 aacccatcaa ttcccggtgc tcaagcacct ccgccggcgt gccgttgaac cgcctcaatt
    13021 cctcgaagaa gtcctcctcg gggagattgt ggattgacgg cacgcttttc ggtatctgtg
    13081 gggcgcgatg accggagacg aagaggcgca gcggcgccgt ccccgtctcc cggcgcagaa
    13141 gacgcgcgag ttcgaaggcg atgacggctc ccatgctgtg gccgaagaga gcgaacggtt
    13201 tgtccaggta cggccgcgcg gcgtcggcga tctcccgcgc gagagtcgag agacgggtgc
    13261 gaggcggctc cttcatgcga cgcccccgtc cggggagttg cgcggcgcag acttcgatgg
    13321 tgctcgggag atgactcggc caccggcgga agatggtttc tccgcctccc gcgtatggga
    13381 agcagaagag acgaagctcg gcttgcgggt tcggctccgg ggttgtgaac cagtcgggtg
    13441 tgttgcgaag agttaccatg attgctgctg tctggctctc aactcggcgc gcgcagtgcc
    13501 gagtcgctga tgaggcatcc tacttttatt caagacgaaa ccgataggag aatgccacaa
    13561 ttctctcact tgagtaattc aaaaattgcc acgccgcgcg cctctgccgc cgcgcgtgcc
    13621 cgtgtaatca cgacgccgcc gtctcattcg agcgccgcga cgctgtccgc ccacagtttg
    13681 atttggcggc cgtcttggtg ttaccaatgt cttccatgtc ggtgctgctg atcttcgtgg
    13741 acgggttggg tatcggcgcc cgcggccctt tcaacccgct cgaccgtctc ggcgacgagg
    13801 cttccccgct cgccgtcttt gaagacgaag agccttccct cccacacggc ggagtgctcg
    13861 tccgcacgga cgcgcgcctc ggcgtcggcg gccgaccgca aagcgcctcc ggccagacga
    13921 ctatcctgac gggcgtcaac gcgccggccg cgctcggcta ccacaagcag ggcttcccca
    13981 acgaagcgat gcgcgagatc atccgccgtc actccatctt ccttcaactc gaacgcgccg
    14041 gggtgcgccc caacgtcttc gtcaacgcct acacgcgtcg cttcttcgac gagcgacccc
    14101 gttgggtctc ggccacgaca gtcgccgtcg aggcggcggg cctccccttt cgcaccgtcg
    14161 aagacctgcg cgcgggccgc gccgtctacc acgacttcac gaatcgcctc atcaccgagc
    14221 gcggcgagga cgttccgccg cgaacgcccg aggaggctgc cgccgtgctc gccgccgtcg
    14281 cgcgcgcaca ccgcttcacg ctctacgaac attttttgac tgaccgcgct ggccatatgc
    14341 aggacgcgga gctggcgcaa agagtcctgt ccgacctcgc gcgctttgtc cgcgcggtgc
    14401 tcgacctagt cgatcacggc gcgacgaccg tcatcctgac cagcgaccac ggcaacgtcg
    14461 aagacctctc cgcgcgcaat cacacgctca actccgtccc cactctcgtc tgggggccgg
    14521 ggcgtgagcg agtcgcacgg cgcgtccgca cgctcgccga catcacgccc acgatttttg
    14581 aaatccttac cgaaggagaa ctcgccgcgt gagccacgaa acccacatgg cacgggagca
    14641 gcagagcgcg gaggtcggcc ccggcggaga aatcgtcaac gaccggcgcg agattttcgg
    14701 ttggatgatg tacgactggg cctgctcggt ctacaacacc actgtgatta cgacgctgct
    14761 cggcctctat ctcaccgagg tggcccaacg tgcggtcggc ccgcgaggct caataatcac
    14821 catcggcggt taccgtgttc tgacggccga gtcgctcttc ccctactgca tcggcctctc
    14881 cgtcttccta caggtctttc tcctgcccgt gctcggggcc atcgccgact tctcgaacgt
    14941 caagaagcgg atgatggcgg ccttctgcta ttcggccgtc gtcgccacct gcctgctctt
    15001 cttcgtcacg gagaaaactt accttctcgg cggtctgctg ctcatcatct cgaacctctg
    15061 cttcggcgcg gcgatggtct tctacaacgc cttcctcccg gagataacga ccgaggacat
    15121 gcgcgaccgc gtctccagcc gcggcttcgc gctcggatat ctgggcggcg ggttgctgct
    15181 cgctctcaac ctcggaatgg tcgcgggcgc tgaccgattg ggcattagcg gagggatggc
    15241 ggtgcgcctg tcgctgctct cggcgggggt gtggtggggg gtgtttgcgg cttacgcgtt
    15301 caggcggctt cggagccgcg cggcggcgcg tccgttgcca gccggaacat cgtatctgac
    15361 ggtgggcttc tcagagcttc gtaaaacctt cgcggagctg cgccgtctgc cgcacacgct
    15421 gcgctacctg ttaggctacc tgctctacaa cgacggcatc cagacggtcg tcactgtggc
    15481 cggcgttttc atcgagcagg agttgttcaa gtcgcgtggg ctggagccgg atcggtcgtt
    15541 cctcatcagc ctcatcctcg tgattcagtt cacggccatc ggcggcgcgc tcctcttcga
    15601 gcgcgtggcg cggctggtgg gaacgaagcg ggcgatcatc acggcgctcg tgctgtggac
    15661 gtgcgtggtt ctttacgctt acggcttcct gcaaacgaag gcgcacgcgt gggcgctcgg
    15721 ggcggcgatt gcggtcgtga tgggcgggtc gcaggcgctg tcgcgctcgc tcttctcaca
    15781 gatgatcccg cgcgggcgcg agtcgtcgtt cttcggcatc tatgaaatct ccgagcgcgg
    15841 cacctcctgg atggggcctg tgatcttcgc cctcgtgctc gactcgaccg gctcctatcg
    15901 caccgccctc ctctcgctcg tcttcttctt cgccgccggg atggtagtac tcctgtttac
    15961 gaatactgcc cgcgccatcc acgacgccgg aaaccttctg cccgaagagg tcaaacacga
    16021 aacggccccg gcataaagaa aagttcaatc gggcggtcac gtaggacagg cattcctgcc
    16081 tgtcttgagc gcggagcgcg aataaactgt tcgcgctccg ctcggcgatg tgagtcggcg
    16141 aatccccttt cgcgctggcg cgctcatgac aggcaggaat gcctgtccta cagcgtcagg
    16201 aggctgagcg gcgcgtcgtc gccggggcgt tggccgcgtc ttcgaggcag gtgtgtgagc
    16261 agaactgggc ggagcgtcct ccgagtgaga cggcgcgcga cgcgggtatc catgtgccgc
    16321 aggaggcgca gcggacgagg ctttcagcgg ccgactggtc gccgacctgt gcggcgtcgc
    16381 ccgcaccctg cgtgcgttgg ccgactcgca ccgtcgcgcc cctggcgacg ccgaaaattt
    16441 ggcgcaccgc cctgatgtac gggcgcagcc gccagtaggc gtagacgccg accagcgcca
    16501 gcattagcag aaagatgaag agtagtttca ctcgcgcgcg tggccccgcg agcgagtgta
    16561 acaaaaaagg gatgtggatg gcgacgcggg gttggcagga ggctccgtgc gggcggtgct
    16621 ccggaattga aagcgcgccg ccgcggcaac aaggccgcgc gccgcgcatc gtttgagatg
    16681 gttctaacgg aggaggatga ctactcttct tccgagttgg ccgagtaagg cagatcgtct
    16741 ttacgccggc gtgggagcgg gtcggaggcg cgacggcgcg acggcgagcc ggggatgcgg
    16801 cggtcaacaa agccgggtgg gaggcgcggc ccgttgacgg cctcttccag tatgcgtgtg
    16861 atctcctgtg agagcgtgcg gtgctcggcg gcggcgcgtt tgcgtagaga ctccttcagg
    16921 tctgacggca cgtaagcccc gatgaagact cccttccggt tggccatcct cgattctcct
    16981 cagaaaaagt ttgggcggcc ctgaaatagc gcgccgcgcg cgtggtcgaa ttggtgcgag
    17041 gagccttgca aatggagagc aggtctgtaa gccgaattct gttccccgac cacgtgggcc
    17101 ggggtggcga tcattcatct agcccgaacg ttgccgttcg ggtcgagcga cctacccgga
    17161 gacttagcgc gccacggcgg cgcgactcgg acgggcagcc cttgttcagt ctccctattt
    17221 ggtcttgcac tgcgaggagt ttgcctagcc acacttgtta ccaagcgtgc tggtgcgctc
    17281 ttacattaag ccccgagggg ccgcaccgtt tcacccatca cccgctgccc cttgcgaggc
    17341 ccgctggctg gtctgttctc tgttgcactt gtcgtcaccc ggcaagtact ggcggcgaac
    17401 ccgaaggccc gcccgttcct cgcccggcgc cctgccgtta gcaggctcgc tgccctgtag
    17461 tgttcggact ttcctccccc cacgcccgaa ggcgccggcg gcgaccgccc gacttgctct
    17521 ccatttgcaa ggccggcgtg attatagcaa accatcgttg aacacgtaca gggacgggtt
    17581 aaaccttccg cgcgtcctcg attgcatgac aaacgagttg cttcaagtct gttgtcaggg
    17641 tttgatggat cagccgaagt atttgtatcc cacgcggagg ccgccgagga gttgtggcgg
    17701 gagcgtcgag acaagcaccc acgtcatcag cgcgtgggag acggcgagcg cggggaggtt
    17761 cggcgcgcgc tggtagacgg cggcccagac gagaccgccg gcgaaggtga cggccgtgag
    17821 agccgggttc gggaaatggg ccagcgcgaa gacagccgat acgagcacga cgctgcgcgg
    17881 cccgcggccg cagatgattt gcgcgcgtcg gttgatgaat gcctgtaggg catactgctg
    17941 caccagcgcc cacgcgagga cgaacgtggg cacgccgacg aaggcccagc cgccgcgcca
    18001 ccttgaaaaa tcaatcgagc cggcaagtgc gcccgcgagc agaaggaagg tcgtcatcgc
    18061 cagtgtgggc agcgccagca gacgcatcgc gcgtgggaaa ttgtcgaaac gccagccgag
    18121 gtcgcgcgcc gtctctttgc gcacgctttt cgagagcaac atgaaggtga aggcgaacag
    18181 gccgacagcg aaattgacaa gacttgagcc ggaatcggcg agagcgagca cgacccattc
    18241 ggcgatcagg acggagagcg tgactgagac tatctcccac gcggccagcg cgcggtcgtt
    18301 cgcggcagct ttgtcggcgg actcgctcag gtgagtttta cggacggccg ggcgcgaaag
    18361 cggcggcggg ctgaaggtag atttatcctc cagcccgccg ccgctttcgc gcgcatggtc
    18421 gtcggtcatt ggccccggta ggtgttgccg ttgccgtcct gaaaggtgag cgaatgagac
    18481 ggcgagagcg tccggtcggc ctcgatgttc atcttgatgc ggacggcgat cttgagcacc
    18541 gccgtgtcgc ccggcgagag ttccgtgacg cgccagacga aacgctgtgc gctgcccgga
    18601 gcgtcctgcg agttgagcga aggctcggag gagatgaatt caagggcgtc gggcaattgc
    18661 tccgtcagga cgaggttctt cgcaagacca gcgccgtcgt tgacgagcac gagccgatag
    18721 aagatcgtgt cgcccggctt cacgtccgag gttggaagtt cgggccgcac gcgcacgcgc
    18781 ggcgtggcca cggtgagcac cgtgctcgcc tcgctggcca cgcggccggc ggcgagagcg
    18841 cgcgtgacga cgttgtaggc gtactgctgg cggtcgggcg tcgagcgcgg gatgtccacg
    18901 cgcagaagta tcgggtactg cccgccgcgc ggctcgatct gcgcggtctg cgtcaccgaa
    18961 ggctcgccct cctggtgcag gccgtcgccg ttcgtgtcgg cgtagacggt cgcggtgggc
    19021 gcgccgggcg ccgtcaccct gacctcgaac gactcgggat agttgccggg gttgcggacg
    19081 atgaacggga gatagatggt gtcgccgggc gtcgccgtca gccccgtcgt gacggagtcg
    19141 atgcgcgcgc gcgcgacgcg gcccacggtg atgctcggcc cgtcgaagtt cgcggggatg
    19201 atgagactct gcgtcttcat cgtgccgcgt ccgacggagc gcgtggcggc aagcgcctcg
    19261 gggacggcct tgagcgtgac gctgatgtcg cgcccctgcc gcgaatcgac ctcgccgaga
    19321 ttccagatgg ccgtgcgcga ggggcggtcg taaacgagcg gactgggatt ggcgctgact
    19381 aattcgaagt cggggttgaa aacgaaatcg acgcgggcgc cgcgcgcggg cgcgctcccc
    19441 tgattcttca tcgtgatgtt ctgcgtgaag gtctcgccgg gcatgaccga ctcctgcgag
    19501 agatcggttg tggcgatgag cgcggcggcg gcgacggtca gagcggcgtc ggccaccttg
    19561 aagacctgat ggtcggtctg cgaggcggcg cggacgaaga agctcagttg cttgttgtcg
    19621 ggcgcggcgt cggggatacg caggttaagc agcacgtcgt agttggcccc gcgcggcagc
    19681 ttcggcgtga ccatgatggg cacttccgta cccgcccctc cctgcgcggc gctgaaggtc
    19741 ggctgaaact ccgcgggcag gtcggtctcg atgctgaact ggtcgtcctt gttgccggtg
    19801 ttggtcacga cgaggggcaa ctgaaccgtc tgccccggcg aagcggagat ggccgcgcgc
    19861 cgcaacgcca acacgaggtt gggaacctgc ctgacggtga tgacgaagct cgacgactgt
    19921 ggcgaggagg agtcggccgg cgccgtcgat gttctcaaag tggccgcggg gtcgctcgtc
    19981 tcgggaacgc gcgccggctc gacggacggc gcggtgacgg gcggcggagt ggtgttcacc
    20041 ctcgcgactt cccgagtgtc gcgacgtctt gacggcgtcg gggtcggcgt cgccctcggc
    20101 gtcggttgca cggcggcggc ggcggtgtcg gccggaatgt tcgatcccct gcgcgtgccg
    20161 gcgcggcgcg agcgctcgct ggttccctcg ctccggggaa cgagtctggg cgttctggtg
    20221 tcggtgacag ccgtcgtccc cggcgtgctc ggctgggaga cggcagcggc ctctctcgcg
    20281 gccgggcgct cgggtgtggg cgtggccgca gcggccactg ccggcagcgg cgtcgaatcg
    20341 atgcgcgggc gtgtcgccgg cgcgccgggc tgtgtgacgg gcggacgacg cgcaatctcc
    20401 tcgcgggccg tcttcaactc gctctcgtag atgtccacga acggggtctc gcggatggtg
    20461 cggaggcgct ggcccacctg ctcggcatcc tggatgcgcc ccgtctgcat gtaggagcgt
    20521 ccgagccaga gcagcgtcag atcggagatg acgctgttcg ggtaggtctt caggaaatct
    20581 ctgaggacag agatggcctg ctcgtagttg ccctgaatga aaaggttctg cccccttgtg
    20641 aacatactct gctcggcgtc gctcggcccg tcgtcctgcg cgccggcggc cgtcccggcg
    20701 gcggcgcgca cgcccgcgac gttggcgaag ggggcgaaga cgagcagcgc cgcgagcgcc
    20761 gcggcgctga ttttattcct tttcatcaag attgtgatcc tccggcaggt aggtgcgagt
    20821 tgataatgct gtactggaaa ttttcgcgac ggttcgggga gtcgcgcggc gaaatctgtt
    20881 tgcgaaccgg ccagcgcgat aatacatcca cacttcggcg acgggcaagg tgcttgttca
    20941 cggggcgccc ctcgccacgg ccggtgctgc taattcttct tcttccccgc cgcctcgacc
    21001 tcgatgcggt cgcccggctg gagagcgggg tcggggattt ttccttcctg tatgaggcgc
    21061 aggtcgtagg tggccggaac gagtcgtcca tcggcgctct gtcgggagac cctgacagcg
    21121 ccggaggacg tgcgcgtgac gccgcccgag gcgagcagcg cctgtgtgag tgtcatgtcg
    21181 gggtggtagt tcttctggcc cggcgcgctg atctcgccgc cgatgtagaa gaactcctgc
    21241 gggcgggcga cgaggttgac gacatcgctc gggtggatga gcgtgttgag gccctgcgtg
    21301 tccgagaggt cgagggtctt gctctgtccc gtcgagtgcg acatgacgag cgcgcggccg
    21361 gcctccggct tcggctgcgc ctcggcgatg aggacgtaga gcgggatggc ctcccgccgc
    21421 aaaatcttca cgccggggtc gtagactaat ccgctgacga gcacggtgtg gctcccgtac
    21481 tcgcggacac tgacgtagac ctgcggcttg tcgtagacgg cgcggcggcg cagctccgcg
    21541 gcgactctca cgcccacctc atcggtcgtc atgccggcga cgttgaaggg gtcgcccgcc
    21601 agcgggtagt cgatgagtcc gccggccagc acggcgaaga gtgtcgattc gcgcggcccc
    21661 acgttctgaa ggcggatgtc gagcacatcg ccgacgccga cgcggtagat ggatgtgggc
    21721 gcgggggcgt gcggcggcgc ggcggcgttg atgttggaga aggaggctga gccgggggcc
    21781 gctgaggtag cgccggcggg agaagccgtg ccgccggccg cgggcgaacc cttgtcgggg
    21841 gcgggcgtga ccttcggaga gttgacggga ggagtcgtgc gttcggacgt ggcgccggtc
    21901 gcgtcgctcg tcatctgcgg cgccgcggtt gcggccgcgg ggttcggagt cgcgttgctg
    21961 gcgcgttcgg tcgcagactt cccggcgtcg ccggtcttgg aatttttggc gttcttcttg
    22021 gggtcgattt tagcggagtt gccggacttg tcagtggcgc gctcgtcgtc cttcttcgcc
    22081 accgcggagt cgttgggggc cgactcgtca gcggcggggc cgacgcgcac gctcggcgtc
    22141 tggggccgtg ccgccgagac gtcggccgcg aaggcggcga tgatgacgcc gagcagaact
    22201 tttttcaagt ggcactcaat ttccatactc gctgaagcga cccggcttcg cagggcattt
    22261 gcggccggtc ggtctccctc cgcaggctca aatcttccga ctcgtgggag gcacgggcaa
    22321 cgcgccgccc acggttgatt caaatgtcta aaagtccgta ttttgtagaa acatcggcgc
    22381 gcaaaaggat aggacagttg gcgcggcccg gagtgtcgga gtgcgaggcc gggagacgtg
    22441 ttggagggct tacggccgtt cgcacctcca ccgactttga ttctacatcc cggatttcga
    22501 tggtggcaag gtttccggcg gaaaaaggtt cggccaaagc tcgcccagac gcacgcgcac
    22561 gacctcctcc gcatcgtccg ccgtctcaca ctcctcgcac tccttcgcga cgcccgcggc
    22621 cctttcggcc tccaccccgg ccagtgcgcg ccgcacacgc ggaatcgacg acggcgtcat
    22681 gctcaactgg cgcgcgccga ggcccacgag gacgacggcg taggccggcg tcgccgccat
    22741 ctccccgcag ataaccgccg ggatggccgc cccctcggcc gcgcgcagcg tgcgccggat
    22801 gctctgaagg acggccgggt ggagcgagcg gaaccattcg gcaacctcgt cgttgccgcg
    22861 gtcaacggcg agcaggtact gcacgagatc gttcgtcccc aggctgaaga aatccacctc
    22921 gcgcgcgaga ctctccgcga ccagcacggc cgacggcacc tcgatcatcg cccccacgcg
    22981 caccttaccg gcctcgcagc cttcacgcgc gagtcgcgcg ccctcctctt cgatgacggc
    23041 gcgcgcgcgg cgaacgtccg tcacgtcgga gatcatcggg agcacgagat cgagcgggcc
    23101 gcgcgcggcg gcgcggagca cggcgcgcgc ctgcgtgcgt agcacgtcga ggttgcggag
    23161 gctgtagcgg atggcccgca gaccgagcgc ggggttgtgc tcgccggagc cgtcgaagtc
    23221 ggcggagagc ttctcgccgc cgaggtcgaa gaggcggacg cacgcgccgt cggctccgcc
    23281 aagttcggcg acctcgaggt aggcgcgata ctgctcctcc tcgccgggcg gcgcgccgcg
    23341 ctgcgtcatc aggaactccg acctgtacag cccgacgccc ctcgccccgt atcgactcac
    23401 accctcgaac tcggccggca gctccacgtt cgcgcgcagg atgatgtcca caccgtcgag
    23461 cgtcagcagc ggctcgcccc gcccgccgtt gccaccggcc gcggcaacgc ccccacgcgt
    23521 cgcaggctcg gccgactcgt ccgtgcggta tcgccccgag gtcacgccgg aagggtggag
    23581 cacgacttcg cccttgcgcg cgtctaccac gatttcgtcg ccggtgcgcg cgcggcggta
    23641 gagatcgcgc aggccgacga tggccgggat gcccatgccg cgcgcgatga tcgccgtgtg
    23701 ggaagtccag ccgccgccgt cggtggcgat ggcccgcacg cgctcgaagt ccagctcggc
    23761 ggccgccgac ggcagcaact cctcggcgac gatgaccgcg tcctccatga cgcgccgcgc
    23821 cggcatgctc tccccgctca gggcgacgag cagccggcgc gtcacgtcct cgatgtccga
    23881 gctgcgctcg cgcaggtagt cgtccttgat ctcggcgtag acggccagca gccggtcggt
    23941 gacgaccttg acggcccact ccgcgttgac ccgcccgccg cggatgtgtt cctcgatctc
    24001 ttcgaggagc cgtcgatctt cgagcatcag caggtgcgcg tcgaagatgt aagcatggcc
    24061 ctcgcccagc gccttctcgg cgcgtctctt aaccacctga agttgtcggc gcgcgagcct
    24121 gacggcggcg cggaagcgac gcgcctcgcg cgcaacctcc gtctcttcga gtgacgcctt
    24181 gtagacgtgc tcgcgcgcgc cgctgtggat acgcagcacg cgccccgccc cgacgccatc
    24241 cgagacggcg acgccgcgca gacgctcctc agccctctcc ttcctctccc tgcggctcaa
    24301 ctcttcggcc ttccctacag cgcccgcgcg actcacgccg ggcgcggctc ctcctcagtc
    24361 tcaccgaagc cgtccgcgat taaagcgcag acggcttcga ccgcttcctg ctcgtcgtcg
    24421 ccctcggcgc tcacgcgcaa ttcagttccg cgcgaggcgg ccagcatcag cacgctcagt
    24481 atggacttgg cgtcggccga ggcgctgcgg tcggcgcgct cgacccgaat ccgacagcgg
    24541 aaggtacacg ccgtccgcac gagccgggcc gcggcgcgcg cgtgtagccc caggcgattg
    24601 acgacgcgga cgctgcactc gaccaagcgt cacccctttt tggcgccggc caaaagctcg
    24661 cccgcgagat agatgccctc gcggccctga tcgcgcacgc ggcgggccac gtcggccagc
    24721 gactcatcgc ccgactgcga cgcgagcttg atgaccatcg gcaggttgac gcccgtgacg
    24781 acctcggcct cgccctcctt gagaaacata gaggcgatgt tcgtcggcgt cccgccgaag
    24841 aggtcggtga gcaggagtac gccgcgtcct tcggagacgc gcgtcacggc gcgttcgatc
    24901 tcgtcgctgg cggcgtccac gtcgtcgtgc cagccgatgg agacggccgc gacgtgactg
    24961 atcgtcccga tgatcgtctc ggcggccgtc agcagctcgc tccccaaacg cccgtgcgtc
    25021 accaacacgc cggccacgcg gcccggcggc gcggcgcgct cgccctctct cttctcactg
    25081 ttgtcgctca tcgattcact ccgcaggccg ccggtcacga ccgtgggggc aggacaagtt
    25141 cgcggcccgc ttactttggg aacaattcaa acatggatgg acaggatgtt caggatgaag
    25201 aaagagctga tttgagattt cagattggag atttcagatt taaattcttc atgtcctgcc
    25261 catcctgtcc atccatgtta attctttttt cgctggaacg ggccgcggac aagtccgcgg
    25321 cccctacagg gttgcgaccc gctttgacta ctgctgccgc ttcgccttcc gggtcttttt
    25381 cacgtcgcgg tgcgtggcgg ccgcggcgaa gccggcgcgg cgcagacgcc gggcgagcgc
    25441 gttggcaatc atcacggagc ggtggcgtcc gcccgtgcag ccgacgccca cggtcacata
    25501 cgacttgccc tcgcgccggt agagcggcag gaggtagtcg agcaggtcgc cgaagcgacg
    25561 gagcgtctcg cccacttcct cgctcgcgtt caaataactg acgacctcgc cgctgtggcc
    25621 ggagaggtgc cgcaactccg ggacgaaatg cgggttcggg aggtggcgca cgtccacgag
    25681 caagtccagc tcgcggggcg cgccgtactt gtggccgaag ctcaagacct gaacgcgcat
    25741 cggcgtgccc tcggcgtcgg ggctgaagcg ttcgacgagg aggcggcgca ggctgtggac
    25801 ggtgtgctcg gaagtgtcga tgacgtggtc ggcgaggccc cgtatctcgt ccatcgcctg
    25861 ccgctcgctg cgaatcgaag aggcgaggcc gtcgcccgtg tcgaacgggt gtgggcggcg
    25921 cgtctcgctg aagcggcggt ggagcgcgtc gtccgaggct tcgaggaaga tgacgaagac
    25981 ctttagccca ctccggcgca gcgccgcgag gcggccgggg aactcactga gaaactgccc
    26041 ttcgcggatg ttgatggcga gcgcggcgcg gccgatgtgc gggcgctctt cgtcgtcttc
    26101 ctgaacgagc cgggcgaaag tcggcaggag cgtgagcggc aggttgtcca cgcagaagta
    26161 gccgaggtct tcaaaggcgt tcgtggccga agacatcccc gagccgctga ggccggtgat
    26221 gatgacgatg tcgggcgcgc cgtccccggc gccgttgttc acagccccac cgcccccggc
    26281 cttccgcttt cttcttttct ctgtcatcaa cccggatgct cggcgccggt gtctgcaccg
    26341 cgaccttcct gcttatcgcc gtcacctatc gcagcggagt ggcgcgcgac gaactccctc
    26401 gccgcattgt agccgcgcac gcgcagaagg tgcacgcgca cggctgcctc cacgagcgtc
    26461 gagaggttgc gtccgggact gacggggata atcacgcgcg gcacttccac gcccagtatc
    26521 tcgactctcg cggcgtccag ccccagccgc tcgacttccc ccgcgtcgtg ccaacgctcc
    26581 agactgatcg acaggcccac gacgacgggg ccgctcgtcg ccgtcacccc gaacaactcg
    26641 cgcacgttca ggatgccgag gccgcgtatc tccatatgct cgcgaagaag ctccggcgcg
    26701 cttccgcgca gcgtgcccgg cccggcccgc cacacctcca ccacgtcgtc ggccacgagc
    26761 cggtgcccgc gcatgagcag gtcgagcgca cactccgact tgcctatccc cgactcgccc
    26821 tcgatcaaca cccccaggcc gtgaatctcc agcagcacgc cgtgccggcg ctcacgcggc
    26881 gcgagcactt cctgcaagta ttccgtgatg acggcgatgg ccgtggaggt gatgagcgga
    26941 ctctggacga tggggagcgc cgcacgttcg gcgacctcgg agagttccga cggtggcagc
    27001 agtcccttcg tgatgaggat gcaggagatt ttttcgagtt cgagattgcc gacggcctcg
    27061 cgccgtccct ccggcgtgag ttgcgagaga aactgcactt cgctctgccc gatgagctgg
    27121 atgcggccgg ggtggatgta gtgtgtgaag ccggcgaggg cgaggccgag tttctggatg
    27181 cgcgcggcgt cgaggcggcg tgtgccggcc ccggccgccc cgttgatgac gcgcaagccc
    27241 agttgcgccg gcgcatcggc gacgaactcg gcgacggtga cggaggggtg ttcagtcata
    27301 agaacaagga tgaaggcgga gggatgaggg atgaagttaa gacaagttgt cttctttcat
    27361 ccctcatccc tccgccctca tccctgcctt tactgcgggc tggcggcccg tgtaatcagg
    27421 ttcaattcga cctcgaccgt atcgccgagg aaagtcggat tgtcaggtcg cgcgaagttg
    27481 atgccgaagt cgcggcggtt gagggtgacg gccgcccgcg cgccgagcgt ggtcttgccg
    27541 gtcttctcgt ccttcgtcac gccgttgacg gtgaacggca gcgcgatctc cttcgcgacg
    27601 ccgtgcatgg tgaaggcgcc gtgggcgatg aagtttttcc ccttcttctc gatgcgcgaa
    27661 ctcttgaagg tgatctcggg aaacttctcc acgtcgaaaa agtcggggtt gcggaggtgc
    27721 gcgtcgcggc gctcgatgcc ggtgtcgatg ctggcggcct tgatgacggc cgtgacggac
    27781 gacttggtca cgtccttgtc gtcgtagacg atggtcacgg cgaagtccgt gaacttcccg
    27841 cgcacctgcg acaggccgcc catgatcggg attgagaaac ccacgttcga gtgggccgtg
    27901 tcgatctcgt acttcgtggt cgcgcccagc acggggctga cgacgagcag aagggcgacg
    27961 atgacgatga aggcacttct tttaagcatc aataatcctt tcgtggttgt cagtagtcag
    28021 tggtcagttg tttgtatcgg ctgcgcgccg cactgaagac gaatgctcat gccacggtac
    28081 gagaaacaac tgaccactga ctactgacca ttgacagtct tttacggctg tatcagtccg
    28141 aaatttccgt ccttgcgctt gtagatgaca ctgaggctgt cggtgtcggc gtcgcggaag
    28201 acgaggaact ggttgtcttc gtcggagagg gcgagcacgg cctcctcggc ggtcatcggc
    28261 ttgacgcggt agcggcgggc gttgatgatg cgcgggccgg cggccttcgc ggcctcggcc
    28321 gtctcggcgg cgagttgttc ggtggccggt gtcggctcaa gcgccgagag cggcgtggcg
    28381 ctctgccgtc gcccgataat cttcttcttc aggcgcacgg cctgcttctc gatcttgtcg
    28441 atgcagcgcg tcagcgcctg atacatgtcg gcgttcgtgt ctttggccgt cagcgtgtgg
    28501 tcgcgccagt ggaggaggat ttccgcgttc tggcggttct tttccacctc gatgatgacg
    28561 tgggtgctga ccgtcgaaga cccctcgaag agatggccga tcatcccgaa atgctcctcg
    28621 acgtggcgcc ggatggcggg cgtcacctcg acgtggcgcc ccgtgtactc gaacctcatc
    28681 agtgcttccc ttccgccggt gtcagcccca aacgcacacg cggggacact tctgctcccc
    28741 accgcaccga ctcgacatgg aatgtgtatt acttgaacct tcgcgaacgg cgcggctgaa
    28801 aaggaagtca ggagccagaa gtcaggagtc agaataaatt agcaaccatg cttttcttct
    28861 gactcctgac tactgacttc tgactcctca ttcagactac cgccttgcgc tcgcgcgagc
    28921 cggggatcga catctggtcg cggtacttcg ccaccgtgcg gcgcgacagc ttgatgccct
    28981 ccccgatcaa aatcttgacg atctgatcgt ccgtaatggg atttttggaa tcctcttcct
    29041 cgatcagctt tttgattcgc agcttgatga tgcgtgtcga gacctcttcg ccgtcctcgt
    29101 tcatcatgcc ttcggtgaag aagcggcgca gctcgatgac gc
//
LOCUS       DASVES010000227         8813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1669938, whole genome shotgun sequence.
ACCESSION   DASVES010000227 DASVES010000000
VERSION     DASVES010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669938"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..754
                     /gene="rnk"
                     /locus_tag="VF240_09320"
     CDS             311..754
                     /gene="rnk"
                     /locus_tag="VF240_09320"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEX7175445.1"
                     /translation="MKVVEADMVKEATIYITEVDRQRLEKLVEIADERDRLASDENLR
                     RLEHELERAETVAPEVVPADVITMRSKVSLRDLNTGEEMVYTLVFPTEANFDEGKISV
                     LAPVGTALLGYRVGDLIEWQVPSGLRRLKVEEILYQPEAAGEYNL"
     gene            complement(822..1916)
                     /locus_tag="VF240_09325"
     CDS             complement(822..1916)
                     /locus_tag="VF240_09325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7175446.1"
                     /translation="MTNNSKSIPQPRKGQGDVKLSREEFIRRLSERFYDPAFDAVRSE
                     IDRVIEVAWTNYDEYHKSPRKRKAGPGFHDPDFELPVEWLEAREQILLAEERQRDPAG
                     PSRILLVCGAARHDQTCPGEMSKTFRLVQMARQEIEGAEGFECDLLDLSMLTAQYGRQ
                     ILPCKACVSTAMPLCHWPCSCYPNHAMGQVNDWMNEIYPRWVAAHGVMIVTPVYWYQS
                     PSVLKLMMDRLVCADGGNPDPTTTHGKNPEEAKAIELQGWSYPRHLAGRLFSVVVHGD
                     AAGAEVLRRSLTDWLLDMNLVQAGHAATIDRYVGYYEPYATSHDALDRDEAFHAEVRN
                     AAKILIEAVGLARAGKLPQPGERVQDPRPK"
     gene            2386..3531
                     /locus_tag="VF240_09330"
     CDS             2386..3531
                     /locus_tag="VF240_09330"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX7175447.1"
                     /translation="MMEMTDVTLTEQLDELAAFEPTEYPFVSLYLNTQADQHGRDNFE
                     SFVRKEFKARAKAFAPESLELASFERDTARIKKYLRDELRPSANGVAIFACAGAGDYF
                     RAVQLDAPVRRHQLHVYQEPYLYPLARLLDQHPRYVALIADTNSARLFVFGLGKTVSS
                     SEVRSAKVSRTQGRGWSQMRYQRHVDNYHLLHAKEVVEMLGRVVREEAADHVVIAGDE
                     VIRPLLREQLPPHLADKVIDVLRLDIRTPEHEILEATMEALREHNLQTDAERVRRLLD
                     EYRAGGLAVVGVRDTLTALYQGQVDELLLSASAQEIVCDEEDFDEAMADGDLPGQHAL
                     RGREPHLVAADLLVTRARLSGARVTFIEDAALLAGVGGCGALLRYRL"
     gene            4147..4680
                     /locus_tag="VF240_09335"
     CDS             4147..4680
                     /locus_tag="VF240_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEX7175448.1"
                     /translation="MTSAVAAEIEVKPFGIKGEAKDGREIANGLSHVLADTYTLYLKT
                     HSYHWNVTGPMFHTLHLLFEEQYRELWAAGDRVAERIRALGFPAPGSFKEFSKLTYLQ
                     EAEGVPTAAKMIRQLVRDHETAARTARWTLSVARSAVDAPTEDLLTQRLAAHDRAAWM
                     LRSLLADEKMSADEQVW"
     gene            complement(4886..4975)
                     /locus_tag="VF240_09340"
     tRNA            complement(4886..4975)
                     /locus_tag="VF240_09340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4939..4941),aa:Ser,seq:cga)
     gene            complement(5050..5565)
                     /gene="tadA"
                     /locus_tag="VF240_09345"
     CDS             complement(5050..5565)
                     /gene="tadA"
                     /locus_tag="VF240_09345"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX7175449.1"
                     /translation="MRTHESPETDAAEAESGDARWMREAVAAAREAALRGEVPVGAVV
                     VAPDGMSVLAVAGNRTRTDCDPTAHAEVVALREAARRAGNYRLTGATLYSTIEPCAMC
                     AGALVQARIARLVYGARDERAGAVESVFRVCDSGSLNHRLELTAGVLEADCRALMQEF
                     FRQRRKTEVRG"
     gene            5991..7067
                     /locus_tag="VF240_09350"
     CDS             5991..7067
                     /locus_tag="VF240_09350"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HEX7175450.1"
                     /translation="MSSDQRTRTGILPAAGDSRGNAAAAAAAPPTQPTPPRTGRPAVS
                     PETAAPSIHQTVVPDPTKAPLIPRKLTSAELNMALVHFYRGEVQRANTWRNRLDTTTN
                     WAVLTAGATLSFAFSSPTNPHFVILINSLLVAFFLFMEARRYRYYEIWSSRVRVLETG
                     YFSRILAPEAPADDAWAKQLSEDLVRPHFTISEWEAVGRRLRRNYLWIFALLAAAWNL
                     KVYLHPLPAYDFNAFIDRARVGLVPGEVVFMLGILFNTAVAVFAVATIRLKEATGEVL
                     PQHDFPTLRRMTNLARDAAKAPATSAARRRAKRARERTTRTSPRMGQTGEWKRSDIHR
                     SQSPDDIHRSQIPNRESTTEETLK"
     gene            7146..8645
                     /gene="hutH"
                     /locus_tag="VF240_09355"
     CDS             7146..8645
                     /gene="hutH"
                     /locus_tag="VF240_09355"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX7175451.1"
                     /translation="MIELDGQQLTLEEVAAVGAGRVGVSLGAGARERMGASRAVVERI
                     VAEGRVVYGVNTGFGKLSDIRIAADELSELQLNLVRSHACGLGAPLSEEETRVLMLLR
                     ANVLAVGLSGARAVVAETLLSMLGRGVIPVIPEKGSVGASGDLAPLAHLALACIGEGE
                     AFFQGERMAGGEALRRAGVEPLRLEVKEGLALLNGTQALTAVGALALHRAHRLTRAAD
                     VAGAMTLEALKGTPAAFDERVHAARPHRGQQDVAARLRELLAESEIRASHAEGDPRVQ
                     DAYSLRCMPQVHGAVRDAVAHARGAVEIETGSATDNPLVFAETGDLISGGNFHGAPLA
                     YAFDYAGLALTSLGNICERRIERLVNPDMNEGLPAFLTSRPGVSSGFMVAQIAAVALL
                     NECKVLAHPASVDNLPTDGGKEDHVSMGMTAATKLRAIVENVEHVVAIELLCAAEGLE
                     YRAPLRPGRGVVRAHEQVRAHVPRLTRDRPLAPDFERIAAALRSGEFDI"
BASE COUNT         1579 a         2865 c         2878 g         1491 t
ORIGIN      
        1 aacacgctga cccgaatcaa aggccgtcgt ctaatattta gacattcatt gatcgtcgcg
       61 cgcgggcttc ggaggcggtg cgaccgtagg aggcggcagg cccggggggg acgggccggg
      121 tcgccgcggg cggccaccgc aagggcaggg cgcgcgcgcg acgatcaatg gaaaacgatt
      181 cgcgggtttc caacttcagg cgggaaagtt ctgccggggc ctgctcctta gcaacggcgg
      241 gagcaggccg ggcgggacat gttttttata acgcctgatt tgacccgccg cccaggagct
      301 gaggaaagat atgaaagtag tcgaggcaga tatggtcaag gaagcaacga tttacatcac
      361 cgaggttgac cggcagcggc tggagaagct ggtcgagatc gccgatgagc gcgaccgttt
      421 ggcgagtgac gagaaccttc gcaggcttga gcacgagtta gagagggctg agacggtcgc
      481 gccagaggtc gtgcccgccg acgtgatcac catgcgctcg aaagttagtc tcagggacct
      541 gaacacgggc gaggagatgg tctacacgct ggtcttcccg acggaggcga atttcgacga
      601 gggaaagatc tccgtcttag cccccgtcgg gacggcgctg ctcggctacc gcgtgggaga
      661 cctaatcgag tggcaagtcc cgtccggcct gaggagatta aaagtcgaag agattctcta
      721 ccagcccgag gcggcgggcg agtacaacct gtgacgatcc gcgcgccgaa tcacagggca
      781 ggtcgtcgtt gaatttgagc cgcccgcaga cttgatgcgg tttacttggg ccgggggtct
      841 tgcacccgct ctccgggctg ggggagtttc ccggcgcgcg ccagccccac ggcctcgatc
      901 aggattttcg cggcgtttcg cacctccgca tgaaacgcct cgtcacggtc gagcgcgtcg
      961 tgactggtgg cgtaaggctc gtagtagccg acgtagcgat caatcgtcgc cgcgtggccc
     1021 gcttggacca gattcatatc aaggagccaa tccgtgagcg agcggcggag cacctccgcg
     1081 ccggcggcgt ctccgtggac gaccactgaa aagagccggc ccgccaggtg tcgcggatag
     1141 ctccagccct gaagttctat cgccttcgcc tcctcgggat tcttgccgtg cgtcgtggtc
     1201 gggtcggggt tgccgccgtc ggcgcagacc agccgatcca tcatcagctt gagaacgctc
     1261 ggtgactgat accagtagac cggggtgacg atcatcacgc cgtgcgcggc cacccagcgc
     1321 gggtagattt cgttcatcca gtcgttgacc tgtcccatcg cgtgattcgg gtaacaggag
     1381 cagggccagt ggcagagcgg catcgccgtc gagacgcacg ccttgcaagg caagatctgg
     1441 cggccgtact gcgccgtcag catcgacagg tcgagcaggt cgcactcgaa gccttcagca
     1501 ccctcaatct cctgccgcgc catttgaacc agtcggaacg tcttggacat ctcgcccggg
     1561 cacgtctggt cgtgccgcgc ggcgccgcac actaacagta tgcgtgacgg gcctgcgggg
     1621 tcacgttggc gctcttccgc caacaagatt tgttcccgcg cttcgagcca ctccacgggc
     1681 agctcgaagt ccggatcatg gaagccgggg cccgcttttc gcttgcgcgg gctcttgtga
     1741 tattcgtcgt aatttgtcca ggcgacttcg atcacccggt cgatctctga tcggaccgcg
     1801 tcgaacgcgg ggtcgtagaa cctttcggac agacggcgga tgaactcctc ccggctgagc
     1861 ttaacgtcgc cctgcccctt acgcggttgc gggattgatt ttgaattatt cgtcatagag
     1921 gtattgagaa ctgctcacct aatcattatc tcacgcatca agggccgcgg gttataaagc
     1981 attcttcggt gtagcagcaa atcagcgcta agtcctcccc ggcactgact cgtcacactt
     2041 tttacaatcc aactcgacgc ctacccgact ctctcgcccc tctggtgtca acacccgcgg
     2101 gcgcaccgta gaaggcgggt ggggtcgcac gtgctgccgg tcacgacgat cttcatgcct
     2161 atgcattagg cgcgatgagg tggcactggt aagcgtgata tcacgcgcgc cccatgattg
     2221 ttatcactcg acgcttcacg cgccgggatc gaccctcggc gcttccgacc gaggattaaa
     2281 agcaagtgta aacactcata gatcacagga gttaatacgc tatctctcgc cagcgttatc
     2341 gcttaggtta ggggtaacga tttcgccgca ggaggcggtc tttagatgat ggaaatgaca
     2401 gacgtgacgc tcaccgagca gttggacgag ttggcggctt tcgagccgac cgaatacccg
     2461 ttcgtcagcc tctacctgaa cacgcaggcc gaccagcacg ggcgcgacaa tttcgagtcc
     2521 ttcgtgcgca aggagtttaa ggcgcgcgcc aaagcgttcg cgccggagtc ccttgagctg
     2581 gcgagctttg agcgtgacac cgcccgcatc aagaaatatt tacgggatga gttacggcct
     2641 tcggcgaacg gtgtcgccat cttcgcctgc gccggcgcgg gcgattactt cagggcggtg
     2701 cagctcgacg cgcccgtcag gcggcatcag ttgcacgtct accaggaacc gtacctctac
     2761 ccgctggcgc gcctgctcga tcagcacccg cgctacgtcg cgctgatcgc cgacacgaac
     2821 tcggcgcgcc tgttcgtctt cggcctgggc aagacggtga gcagttcgga ggtgcgcagc
     2881 gccaaggtga gccgcacgca gggccggggc tggtcgcaga tgcgctacca gcggcacgtg
     2941 gacaactacc acctgctcca cgccaaagag gtggttgaga tgctcggacg cgtcgtgcgc
     3001 gaggaggcgg cggatcacgt cgtcatcgcc ggagacgaag tgatccgccc actgctccgg
     3061 gagcagttgc cgccgcacct cgctgacaag gtgatcgacg tgctgcggct cgacatcagg
     3121 acgcccgaac acgaaatctt agaggcgacg atggaagccc tcagagagca caacctgcaa
     3181 acggatgccg aaagggtaag gcggttgctg gacgagtacc gcgccggcgg cctggcggtc
     3241 gtcggagtgc gcgacacgct caccgcgctc tatcagggtc aggtggacga gctgcttctg
     3301 agcgcctcgg cgcaagagat cgtctgcgac gaagaagatt ttgacgaggc tatggcggac
     3361 ggcgacttac ccggtcaaca cgcccttcgc ggtcgtgagc cgcacctggt ggctgcggac
     3421 ttactcgtca cacgcgcgag gctgtcgggc gcgcgtgtca ccttcatcga ggacgcggcg
     3481 ctgctcgccg gggtcggggg ctgcggcgct ctgcttcgat accgtcttta atcgcgggca
     3541 cgccgtcttc ggctcgaacg cctaccgggc gctgtgaggg tcgcgcaggc cctttctggt
     3601 gacgcgacgg gcgggtcaca ccgccacgcc tcgtgatgcg caagtctata gggagggtta
     3661 ctgagtgttc acgaagttcc atcacatgct ggcggcgcgc aggcgacggc gggcgctcgt
     3721 aaaaattcgc caagggttcg cgcggtgcgg acaccctttg gacggattgt ccgacgccga
     3781 catagaggcg tccctgccgc cgggcatgtg cgaaacgccg cccgactatc tgggcgcgaa
     3841 gacgatcagc cgcgcgctac ggcggctgtc aaccggcgcc gcaggtgagc acgggcacgc
     3901 acgaagggag gtgagaaggg atgtcggtta tggggggtga ggtcattcgc cactgcgaga
     3961 agtgtgacag ggagagcaac tatctcacca atttttttta gtccgcacaa cggtgccgga
     4021 gctgttctgc tgggagtgtg gagcaggagg atcagcgcag catgaggttt agtccgtcgt
     4081 ggacacgaca gcggcgcgtg atcagtaggg cttattgacg aggacgaaaa gggaggtgga
     4141 aagaagatga cgagtgcggt tgcggccgaa atcgaagtaa agcctttcgg catcaagggc
     4201 gaggcgaagg acggacggga gatagcgaat gggctgtccc acgtgctggc cgacacttac
     4261 acgctgtatc tgaagacgca cagctaccac tggaacgtga cggggccgat gttccacacg
     4321 ctgcacctgc tgttcgagga gcagtatcga gaactgtggg cggcgggcga ccgtgtcgcc
     4381 gagcgcatcc gcgcgctggg cttccccgcg ccgggatcct tcaaggagtt ctccaagctc
     4441 acctacctac aggaggccga aggcgtcccc acggcagcga agatgatccg ccaactggtc
     4501 cgggaccacg agacggccgc ccgcacggcg cgctggacgc tgtcggtcgc gcgctcggcg
     4561 gtggacgcgc cgactgaaga cctcctgacc cagcgtctgg cggcgcacga cagggccgcg
     4621 tggatgctca gaagcctgct tgccgacgag aagatgagtg cggacgagca agtctggtga
     4681 aggcgcgggg tgcaggcggt cgcgcgaccc gcgctcggaa gatctgaagg agaactggcg
     4741 cggcccagtc tcctgtcggc cgaggattgg aggcgagaag tagacgaaaa gaaccaccag
     4801 aggtgggttc gcgtgggggt caggatgcgg caagtaaaat gcggaaaatc gaaacctcga
     4861 aatccccgca agaccttgaa ataaatggcg gagagggtgg gattcgaacc cacggtggcc
     4921 tttcgaccac tccggttttc gagaccggtg cactaaacca ctatgcgacc tctccgtaaa
     4981 gggcgagagg ttagaggtca gaggttagag gttagtaaaa gcaatccgtt atttactaac
     5041 ctctaacctc taacctctga cctctgtctt ccggcgctgg cggaagaact cctgcatcaa
     5101 cgcgcgacag tctgcttcga gcacgccggc cgttagttcc agacgatgat tcaaagagcc
     5161 ggagtcgcag acgcggaaga ccgactcgac ggcgccggcc cgctcgtcgc gcgcgccgta
     5221 aaccaacctc gcgatcctcg cctggacgag cgcgccggcg cacatggcgc atggctcgat
     5281 ggtcgagtaa agggtcgcgc ccgtcaggcg gtagttgccc gcgcggcgcg cggcctcgcg
     5341 cagggcgacc acctcggcgt gggccgtcgg gtcgcagtcg gtgcgcgtgc gattgccggc
     5401 gacggcgagc acgctcattc cgtctggcgc gacgaccacg gcgccgaccg gcacctcgcc
     5461 gcggagcgcg gcttcgcgcg ccgccgcgac ggcctcgcgc atccaacgcg cgtctccgct
     5521 ctcggcctcg gccgcgtcag tttccggcga ttcatgcgtc cgcattcgtg atagacgctt
     5581 tcaagaagca gcaaacgaac cgtgactata ggtgccgtgg gcgcgggctg tcaagcgacc
     5641 cggttttgat gaaacgcggg actttacggc tgcttttggg ccgcgcggcc gacccgcccc
     5701 ggcggcgcga acgcggcgcg gatttccttg cgcgcgcaag ccgccggggc gtgcgagaac
     5761 acggatgaga cggatgaaga cggatgggca cggatgcgat ccgtgtgaat ccgttttcat
     5821 ccgtctcatc cgtgttcctt tgatcctcat ccttgcccga ctgcacaaag cgcgcgcgac
     5881 cacgcaagag tttctacgcc cgccgccgcg caccgtgcta ttatcctgag actgaaggca
     5941 ccgcgtgttg tccctcccgc ctgcgcgccg cgcccgcttc gacccagacg atgagcagcg
     6001 accagcgaac gagaacgggc attcttccgg ccgccggcga cagcaggggc aacgccgccg
     6061 ccgccgcggc tgcgccgcca acgcaaccga cgccgccgcg caccgggcgt cccgccgtct
     6121 cacccgagac cgccgccccg tccatccacc agacggtcgt ccccgacccg acgaaggctc
     6181 cgctcatccc gcgcaagctc acgtccgcgg agctgaacat ggcgctcgtc catttctacc
     6241 gcggcgaggt gcagcgcgct aacacctggc gcaaccgtct cgatacgacg acgaactggg
     6301 cggtgctgac cgcgggcgcc acgctctcct tcgccttctc gtcgccgacc aacccccact
     6361 tcgtcatcct gatcaactcg ctcctcgtcg ccttcttcct cttcatggaa gcgcgccgct
     6421 accgctacta cgaaatctgg tcgagccgcg tgcgggtgct ggagaccggc tacttctcgc
     6481 gcattctcgc gcccgaggcg ccggcggacg acgcctgggc caagcaactc tccgaagact
     6541 tagtacgccc ccacttcacc atcagcgagt gggaggccgt ggggcgtcgc ctgcggcgca
     6601 actacctgtg gatcttcgcg ctgctcgccg cggcgtggaa cctgaaggtc tacctgcacc
     6661 cgctgccggc ctacgacttc aacgccttca tcgaccgcgc gcgcgtcggc ctcgtgcccg
     6721 gcgaggtcgt cttcatgttg ggcatcctct tcaacacggc ggtggccgtc ttcgccgtcg
     6781 ccaccatccg gctcaaggag gcgaccggcg aggtgctgcc gcagcacgac ttcccgacct
     6841 tgcggcgcat gacaaacctc gcccgcgacg ccgccaaggc tccggccacg agcgccgccc
     6901 gccgccgcgc caagcgcgcg cgcgaacgca ccacccgcac ctcgccccgc atgggccaga
     6961 ccggcgagtg gaaacgctcc gacatccacc gctcgcaatc gcccgacgac atccaccgct
     7021 cgcagattcc caaccgggaa tcgacgacgg aagaaacgct caagtagagg tcagaggtca
     7081 gaggtcagag gtcagtcaaa gacctgcttt ttaagtaacc tctgacctct gacctctgac
     7141 ctcttatgat tgaacttgat ggacagcagt tgacgctcga agaggtggcg gccgtgggcg
     7201 cggggcgcgt gggcgtgtcg ctcggggcgg gcgctcgcga gcggatgggc gcgtcgcgcg
     7261 cggtggtcga gcgcatcgtc gcggaggggc gggtcgtcta cggcgtcaac accggcttcg
     7321 ggaagctctc cgacatccgc atcgcggcgg acgagttgag cgagctgcaa ctgaacctcg
     7381 tccgcagcca cgcgtgcggc ctcggagcgc cgctctcgga agaggagacg cgcgtgttga
     7441 tgctgctgcg cgccaacgtg ctcgccgtgg gcttgagcgg cgcgcgcgcc gtcgtcgccg
     7501 agacgctgct gtcgatgctg gggcgcggcg tcatccccgt catccccgag aaggggtcgg
     7561 tcggggcgag cggcgacctc gcgccgctgg cgcacctcgc gctcgcgtgc atcggcgagg
     7621 gcgaggcttt ttttcagggc gagcgcatgg cgggcggcga ggcgctcagg cgcgccggcg
     7681 tcgagccgct ccgacttgag gttaaggagg ggctggcgct cctcaacggc acgcaggcgt
     7741 tgacggccgt cggcgcgctc gccctccacc gcgcacaccg cctcacgcgc gcggccgacg
     7801 tggccggcgc catgacgctc gaagccctca agggcacgcc cgccgccttc gacgagcgcg
     7861 tccacgcggc gcgcccgcac cgcggccaac aggacgtggc cgcgcgcctg cgcgagcttc
     7921 tggccgagag cgaaatccgc gcctctcacg ccgagggcga cccgcgcgtg caggacgcct
     7981 actccctgcg ctgcatgccg caggtacacg gcgccgtgcg cgacgccgtg gcgcacgcgc
     8041 gcggggccgt ggagatcgag acggggagcg ccaccgacaa tccgctcgtc ttcgccgaga
     8101 cgggcgactt aatctccggc ggcaacttcc acggcgcgcc gctcgcctac gccttcgact
     8161 acgcgggcct ggcgctcacc tcgctcggca acatctgcga gcgccgcatc gagcgacttg
     8221 tcaaccccga catgaacgag ggcctgccgg ccttcctcac ctcgcgcccc ggcgtctcct
     8281 ccggcttcat ggtcgcgcag atcgcggccg tcgcgctcct caacgagtgc aaggtgctcg
     8341 cgcacccggc ctcggtggac aacctcccga ccgacggcgg caaggaggat cacgtctcga
     8401 tggggatgac ggccgcgacc aagctgcgcg ccatcgtcga gaacgtcgag cacgtcgtcg
     8461 ccatcgaact cctgtgcgcc gccgaggggc tggaataccg cgcgccgctc cggccgggcc
     8521 gcggcgtcgt gcgcgcacac gagcaggtgc gcgcacacgt cccgcgcctc acgcgcgacc
     8581 gcccgctcgc ccccgacttc gagcgaatcg ccgcggcgct ccgcagcggc gagttcgaca
     8641 tttagatcgt atttgagttg agtgtcgagt agattgggtt caccgcacac tcaatctgaa
     8701 gctctaagta acaacgccgc aaccccgaag ggctgcggcg ttgttcgtct cgcttaatta
     8761 ctaacttcta tcacaagcac gcggcttcgc gctcgcctgc gccgctttct ggg
//
LOCUS       DASVES010000228         6291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_43630, whole genome shotgun sequence.
ACCESSION   DASVES010000228 DASVES010000000
VERSION     DASVES010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43630"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1568)
                     /gene="glnA"
                     /locus_tag="VF240_09360"
     CDS             complement(144..1568)
                     /gene="glnA"
                     /locus_tag="VF240_09360"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX7175452.1"
                     /translation="MNAKTVLKYADDNEARFISVRFTDLVGAWHHLTFPIHELSEDSF
                     RDGFGFDASSLRGWASIHESDMLLVPDPVRMWMDPFTEEPTLCLIANAVDPMTKDGYG
                     LDPRSVAQRAEAYLRFTGIADTVYFGPEAEFFVFDSVNFKNDANAAGYSVDSDEAHWS
                     SGRTETSEFQGPNLGYRVRQKEGYVPVAPVDSLVDLRSEMALTLERVGITVECHHHEV
                     APMQCEIDIRYATMLGTADNLQLFKYVVRNTAYQYGKAATFMPKPLYGDNGSGMHCHQ
                     SLWKGEKPLFAGDGYANLSEMALWYIGGLLKHAAAVVAFAAPTTNSYKRLVPGFEAPV
                     NLAYSARNRSAAIRIPMFSQSPKLKRLEFRPPDPSCNPYLAFSAMLMAGLDGIQNRID
                     PGQPLDKDIYDLSPEELKDVPSLPGSLDASLSALEADHDFLLKGDVFTPQLIERWITY
                     KREKEIQPLRMRPHPLEFSMYFDI"
     gene            1993..3936
                     /gene="speA"
                     /locus_tag="VF240_09365"
     CDS             1993..3936
                     /gene="speA"
                     /locus_tag="VF240_09365"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX7175453.1"
                     /translation="MTTVIEQTLETYGIENWGAGYFGINRKGNLVVHPSENEQGRSAD
                     LKEIIDDLARRGIRTPVLLRFPQLIAAQVRKLQRAFRNSIREYEYAGAHMCVYPMKVN
                     QNRAVVEEYLREGTRYDFGLEAGSKAELYGALAMEQSGESLLVLNGFKDEEFIRLAFV
                     GAQAGKRVVIVIEKMSELDHFLRLAETAGDGDDLPMVGIRVKLYSKGSGRWEKSGGEA
                     AKFGLTTTEILEVIRRMQEAGRTEHLRLLHFHIGSQLTDIKRIKNAMKEAARVYSKIR
                     QMKVPIDLLDVGGGMAVDYDGSKTAFDSSANYSAQEFANDVVYTIMQVCDDENVPHPT
                     IIQECGRYLSAYHAVLVTNIIDEVETEVEEITPMEIVEDDPQVVIELGDLRESMTVKN
                     YREFYHDALEHREELFTLFNLGLINLEDRAKGEVLFWDICERADKYAQTREYVSEEFD
                     ELRRLLRSKYLTNFSVFRSVPDHWALDQLFPIVPIHRLNKAPTDYATLCDITCDSDGV
                     VDKFVDLHDVKQVLELHPLIKGEPYYIAFALVGAYQEVMGNNHNLFGAPNEAHIHIDE
                     DGYLIKKVVKGTTVGEALERARYERGQLHEGFRRQVAQRVKHGELTEAEAAKMVEYYE
                     ARINAYTYLSVNGEVAGQKRRKD"
     gene            4076..5128
                     /locus_tag="VF240_09370"
     CDS             4076..5128
                     /locus_tag="VF240_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX7175454.1"
                     /translation="MVTQRKPRATKYLTTPMRPVQVDRDRSVAGLLEKMEGAGLGARQ
                     LAEAHRIWLDMLGDNTTIIVAASGALIPAGMRRLLAYVIKNRFVDMLVCSGPMLFHDL
                     HETLGRHHYQAHPSMTEAELEASQINRMWDVLASEEEYREADEWVGGFTNQLDQTRAY
                     STREFLHLLGRELAEIATDDGILTSSYKARVPIFCPTISDSAITVGIAGARFEKKNNF
                     CFDLAQDILDVMQVAARSRVTGVINIGGGTSRSFLQQMEAATGIIKQPVRGHKYGIQI
                     SPNAQQPSGRMGSDDTLVFGKLQRGAALAHVNCDATIALPILVTSLSQTAAKYMKGRK
                     RPTFTFAGRDLMVEVP"
     gene            5269..6201
                     /gene="speB"
                     /locus_tag="VF240_09375"
     CDS             5269..6201
                     /gene="speB"
                     /locus_tag="VF240_09375"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX7175455.1"
                     /translation="MSESAQLPMNFGGIEDEALSAFEGARVLVWPISYEGTVSYGGGT
                     GEGAMAIVDASRNMELYDEETDSETYRLGIHTLDVTPSVAPPEAMMKGLLEQARRLVA
                     TEKFVCMIGGEHSVSAPVIQAHAERYENLSVLQIDAHADLRDTYDGTPHSHASIMARV
                     VRDLKIPAVQVGIRSISAEEARVIDEIPTRIFWAKDIVGRTDWWEEAVEGLTENVYLT
                     IDIDGLDPSLVAQTGTPEPGGLGWYETVGLIRTLARQRRVVGMDLNEYAYVEGQTASA
                     FLCAKLIYKSLSYIFESETERVRGAGKQTSRAGA"
BASE COUNT         1215 a         1994 c         1980 g         1102 t
ORIGIN      
        1 cgggccggtg catcacgccc gtcaattctc ttccgcccct ccggggcttc ggtttctttt
       61 ttgactcgtc cccacggctt acgccgtggg ctacattctg ccgccgcctc cggcggctcg
      121 ctgatgctca agcggagtgc actctaaatg tcgaagtaca tcgagaactc caacgggtgc
      181 gggcgcatcc tgagcggctg aatctccttc tcgcgcttgt aggtgatcca gcgttcgatg
      241 agttgaggcg tgaagacatc gcctttcagc aggaagtcgt ggtcggcttc gagcgccgag
      301 agtgaggcgt cgagcgagcc gggcagtgaa ggcacgtctt tcaactcttc gggcgacaga
      361 tcgtagatgt ccttgtcgag cggctgaccg gggtcgatgc ggttctggat gccgtcgagt
      421 ccggccatga gcatcgccga gaaggcgagg taagggttgc acgacgggtc gggcgggcga
      481 aactccagac gcttcagctt cggactctgc gagaacatcg ggatgcggat ggcggccgag
      541 cgattgcggg ccgagtaggc gagattcacc ggcgcctcga agccggggac gagacgcttg
      601 tagctgttcg tcgtcggcgc ggcgaaggcg acgacggccg ccgcgtgctt gagcagtccg
      661 ccgatgtacc agagcgccat ctccgagagg ttggcgtagc cgtcgccggc gaagagcggc
      721 ttctcgccct tccacaaact ctggtggcag tgcatccccg agccgttgtc gccgtaaagc
      781 ggcttgggca tgaaggtcgc ggccttgccg tactgataag ccgtgttgcg cacgacgtac
      841 ttgaagagct ggaggttgtc ggccgtgccc agcatcgtcg cgtagcggat gtcgatctca
      901 cactgcatcg gcgccacctc gtgatgatgg cactcgaccg tgatgccgac tcgttcgagc
      961 gtcaaggcca tctccgaacg cagatcgacg agcgagtcaa cgggcgcgac ggggacgtag
     1021 ccctccttct gacggacgcg gtagccgagg ttcggcccct gaaactcgct cgtctcggtg
     1081 cgcccgctcg accagtgggc ctcgtcggaa tcgacgctgt agccggcggc gttcgcgtcg
     1141 ttcttgaagt tgacggagtc gaagacgaag aactcggctt cggggccgaa gtagaccgtg
     1201 tcggcgattc ccgtgaagcg cagataggct tcggcccgct gcgccaccga gcgcgggtcg
     1261 aggccgtagc cgtccttcgt catcggatcg acggcgttgg cgatcaggca cagcgtcggc
     1321 tcctccgtga acgggtccat ccacatccgc acggggtcgg gcacgaggag catgtctgat
     1381 tcgtgaatcg aggcccagcc gcgaaggctt gaggcgtcga agccgaagcc gtcgcggaag
     1441 gagtcttcgg agagttcgtg gatggggaaa gtcaggtggt gccacgcccc gacgaggtcg
     1501 gtgaatctga ctgagatgaa tctggcctcg ttgtcatccg catacttcaa gacggtctta
     1561 gcgttcataa tgtctcctgg aatctatttg gagggttgac agggtgggcc gccttggggg
     1621 cggctggagc ggcgctggaa actaaccgga ggaggtgcgt catttcacgt cggacgctgt
     1681 aaaaagcagg cattatagtc gggagcgctt gggtgtcaag gtttcacgac ttctgtttca
     1741 cgcgtccgac ggcgcgcccg cgtccgcgtt ttcgccctaa acctttctgg cacgccggat
     1801 taagcgcggc ccgcgtcaaa aaaatagttt ttgacctgcc ccgtatatat ttgctaaatt
     1861 gcctgtctaa gatttcccat cactgattcc atctaaccgt ccgaagggcc cgagcactcc
     1921 gaaacggaag acgagtggcc tcggccggcc atctaacgcg gcgatcaccg gggaggcagg
     1981 agaaccgaga agttgaccac cgtcatcgaa cagactttag agacctacgg catcgaaaac
     2041 tggggggccg gctacttcgg catcaatcgc aagggcaacc tcgtcgtcca cccttcggag
     2101 aacgagcagg gacgcagcgc cgacctcaag gagattatcg acgacctcgc ccgtcgcggc
     2161 atccgcacgc ccgtcctcct gcgcttcccg caactcatcg ccgcgcaggt cagaaagctc
     2221 cagcgcgcct tccgcaactc catccgcgaa tacgagtacg cgggcgcaca catgtgcgtc
     2281 tacccgatga aggtcaacca gaaccgcgcg gtcgtcgagg agtacctgcg cgagggcacg
     2341 cgctacgact tcggactcga agccggctcg aaggccgaac tctacggcgc gctcgccatg
     2401 gagcagtcgg gtgaatcgct cctcgtcttg aacggcttca aggacgagga gttcatccgc
     2461 ctcgccttcg tcggcgcgca ggccggcaag cgcgtcgtca tcgtcatcga gaagatgtcg
     2521 gagctggacc acttcctgcg cctcgccgaa acggccggcg acggcgacga tctgccgatg
     2581 gtcggcatcc gcgtcaagct ctactcgaaa gggtcgggcc gctgggagaa gtcgggcggc
     2641 gaggcggcga agttcggcct caccacgacg gaaattctcg aagtcatccg gcgcatgcag
     2701 gaggcggggc gcaccgagca cctgcgcctg cttcacttcc acatcggctc gcagctcacc
     2761 gacatcaagc gcatcaagaa cgcgatgaag gaggccgcgc gcgtctactc caaaatccgc
     2821 cagatgaagg tgcccatcga cctcctcgac gtgggcggcg gcatggcggt cgattacgac
     2881 ggctcgaaga ccgccttcga ctcctcggcc aactactcgg cgcaggagtt cgccaacgac
     2941 gtggtctaca ccatcatgca ggtctgcgac gacgagaacg tgccgcaccc gacgatcatt
     3001 caggagtgcg gacgctacct gtcggcctac cacgccgtgc tggtgacgaa catcatcgac
     3061 gaagtggaga ccgaggtcga agagatcacg ccgatggaga tcgtggagga cgacccgcag
     3121 gtcgtcatcg agctgggcga cttgcgcgag tcgatgacgg tgaagaacta ccgcgagttc
     3181 taccacgacg ccctcgaaca ccgcgaggag ctgttcacac tcttcaacct cggcctcatc
     3241 aatctggaag accgcgccaa gggcgaggtg ctcttctggg acatctgcga gcgcgccgac
     3301 aagtacgcgc agacgcgcga gtacgtctcc gaggagttcg acgaactgcg ccggctgctg
     3361 cgctccaagt acctgacgaa cttctcggtc ttccgaagcg tgcccgacca ctgggcgctc
     3421 gaccagctct tccccatcgt ccccatccat cgtctcaaca aggcgccgac cgactacgcc
     3481 acgctctgcg acatcacctg cgactcggac ggcgtggtcg ataagttcgt cgatctccac
     3541 gacgtgaagc aggttttgga actccacccg ctcatcaagg gcgagcctta ctacatcgcc
     3601 ttcgcgctcg tcggcgctta tcaggaggtg atgggcaaca accacaacct cttcggcgcg
     3661 cccaacgagg cgcacatcca catcgacgag gacggctacc tcatcaagaa ggtggtcaag
     3721 ggcacgaccg tcggcgaggc gctggaacgc gcccgctacg agcgcggcca gctccacgag
     3781 ggcttccgcc gtcaggtcgc gcagcgcgtc aagcacggtg aactcaccga ggccgaggcc
     3841 gccaagatgg tcgaatatta cgaggcgcgg attaacgcct acacgtacct ttcggtcaac
     3901 ggagaggtcg cgggccagaa gcgccggaaa gactaaaaga cgtcaatcgt gaatcgtaaa
     3961 tcgtaaatcg aaaagcccgc gccgcccgaa cgcctgacct acgattcgcc gttaacgtta
     4021 aagaccgcca accacgattg acgattcacg attgacgatt gacgaggttt taagaatggt
     4081 aacccaacgt aaaccgagag ccacgaagta tctgaccacg ccgatgcgcc ccgtccaggt
     4141 tgaccgcgac cgctcggtcg ccggcctcct tgagaagatg gagggcgcgg ggctgggggc
     4201 gcgccaactg gccgaggccc accgcatctg gctcgacatg ctcggcgaca acacgaccat
     4261 catcgtcgcc gcctccggcg cactgatccc cgccggcatg cgccgtctcc tcgcttacgt
     4321 catcaagaac cgcttcgtgg atatgctcgt ctgctccggc ccaatgctct tccacgacct
     4381 gcacgagacg ctcgggcggc accactatca ggcgcacccg tccatgacgg aggccgaact
     4441 cgaagcgtcg cagatcaacc gcatgtggga cgtgctcgcc agcgaagagg agtaccgcga
     4501 ggccgatgag tgggtcggcg gcttcaccaa ccagctcgac cagacgcgcg cctactcgac
     4561 gcgcgagttc ctacacctct tgggtcgcga gctggccgag atcgccaccg acgacggcat
     4621 cctgacttcg agctacaagg cgcgcgtccc catcttctgc ccgaccatct ccgacagcgc
     4681 catcacggtc ggcatcgccg gcgcacgctt cgagaagaag aataatttct gcttcgacct
     4741 cgcccaggac atcctcgacg tgatgcaggt cgccgcgcgc tcgcgcgtca ccggagtcat
     4801 caacatcggc ggcggaacca gtcgcagctt cctccaacag atggaggcag ccaccggcat
     4861 catcaagcag cccgtgcgcg gccacaaata cggcatccag atttcgccca acgcgcagca
     4921 gccctcgggg cgcatggggt cggatgacac gctcgtcttc ggcaagctcc aacgcggcgc
     4981 cgccctcgcc cacgtcaact gcgacgccac catcgccctt cccatcctcg tcacctcgct
     5041 ttcgcaaacg gccgccaagt acatgaaggg ccgcaagcgc ccgaccttca ccttcgccgg
     5101 ccgggatttg atggtcgagg tgccgtaaaa aaaagggagt caggagtcag aagtcaggag
     5161 ccggaatgag gcggtcggcg tggggccgtc tcattccggc tccgttgtag tttgcgcgcg
     5221 aattctgact tctggcttct gactcctgac ttcctgtaaa ctttccgcat gtctgaatca
     5281 gcacagctcc cgatgaactt cggcgggatc gaagacgagg ctctgtcggc ctttgagggc
     5341 gcgcgcgttc tcgtgtggcc gatctcttac gaggggacgg tttcctatgg tggggggacg
     5401 ggcgagggcg cgatggcgat tgtggacgcg tcgcggaaca tggagttgta cgacgaggaa
     5461 accgattcgg agacctaccg gctcggcatc cacacgctcg acgtgacgcc gagcgtcgcg
     5521 ccgccagagg cgatgatgaa ggggttgctg gagcaggcgc gccggctcgt cgccacggag
     5581 aagttcgtct gcatgatcgg cggcgagcat tcggtgagcg cgcccgtcat tcaggcgcac
     5641 gccgaaaggt atgagaacct gtcggtctta cagatcgacg cccacgccga tttgcgcgac
     5701 acctacgacg gcacgccgca ctcgcacgcg tcgatcatgg cgcgcgtggt gcgcgacttg
     5761 aagattccgg ccgtgcaggt cggcatccgc tcgatctcgg cggaggaggc gcgcgtcatc
     5821 gatgagattc cgacgcgcat cttctgggcc aaggacatcg tgggccgcac ggactggtgg
     5881 gaggaggccg tcgaggggct gacggagaac gtctacctga ccatcgacat cgacggtctc
     5941 gacccgtcgc tcgtcgcgca gacggggacg cccgagccgg gggggttggg ctggtacgag
     6001 accgtcggcc tcatccgcac gctcgcgcgc caacgccgcg tcgtcggcat ggacttgaac
     6061 gagtacgcct acgtcgaggg ccagaccgcc tccgcgtttt tgtgcgccaa gctgatctac
     6121 aagtctctgt cctatatctt cgagtcggag acggagcgcg tgcgcggcgc aggcaagcag
     6181 acctcgcgcg ccggcgcgtg agtgtttgca atggagtacg tgggacagta ccgcgagcgg
     6241 tagcgagcgg gttgacgctc ccgtagctac aacccgctcg ctaccgctcg c
//
LOCUS       DASVES010000229         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_578788, whole genome shotgun sequence.
ACCESSION   DASVES010000229 DASVES010000000
VERSION     DASVES010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578788"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VF240_09380"
     CDS             complement(<1..821)
                     /locus_tag="VF240_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7175456.1"
                     /translation="MVAENIVGTDEELGWRSVRGDFPVMENVVYLNSAASGPVPRPVH
                     RAATGFYAEMLEGGDKLWEEWLERREQARASVARLINADPEEVAFTTNTSSGMNLIID
                     ALGSRGEVVAGELEFPVSTITWMHRGARVRLVKAVNGELLTDDLLDAAGEGDSVICLS
                     HVQYSNGFRADLEEIGRRKGGHPFVVNAAQSAGAFHIDVKRMGIDALCTTGHKWMLSG
                     YGSGFVYLSRELLARTRPRSIGWMSVENPYGMSNTEFVVRPDAAPRAEMGCPHFAG"
     gene            921..2339
                     /gene="lysA"
                     /locus_tag="VF240_09385"
     CDS             921..2339
                     /gene="lysA"
                     /locus_tag="VF240_09385"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7175457.1"
                     /translation="MTTETAERAPAWSIPGFLETREGRLAINGVDALDLTARHDTPLF
                     VFSEPRIRANIARLREAAERVGHRVKYCYASKANSNMAVLAAVRDAGIDVEVNSGGEL
                     FKALRAGFRPDQIIFNGTSKSDAELDEAVGAGIYSTNVDSVYEIELVEAAARRTGKRA
                     RIALRLVPEIGTRSHLGLQTALLTSKFGISSSEVLDAFRRALRHPELVHVSGIHIHVG
                     SQTPDVEPYAAAFGAMWEHLATIHRESGHVLEHINLGGGIPVNYLRDPSQAGDLPEHE
                     RDMLGAELEPADVLAAALQAARDSARAAGAEHLLESVEILLEPGRSVIADAAVLLTTV
                     RNVKHRPETGDVWLLTDAGYNLALSQSMYKWYYHLISASRAAAPHDTAYKVAGPLCDS
                     GDVYFDIERGGRLPDYRKLPADVRPGEVLALLNSGAYTLAQMTTYNGRPLPAAVMVRA
                     GGRVDAARRRDTYEDLLAGEVF"
     gene            2398..3840
                     /locus_tag="VF240_09390"
     CDS             2398..3840
                     /locus_tag="VF240_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7175458.1"
                     /translation="MSQTQQNQTLTLVRGLTLVAAISIIIGNVIGTGVFLKARVMTCN
                     VGSPGMVMTVWVVAGLLSLAGALTYAELTAMMPRAGGEYVFMREAYGPGAGFLSGWMQ
                     ILIAKTGSQASVALIFAASLNDLTGKAVESASAYGVQLTALAVIIAITLLNCAAVKVS
                     GWVATVLTGVKIALVLGIGLGAFFLASGDWGNYALSNLGGTCEGVSPGATLGAAGFTG
                     AMLGALWGYDGWNNLTFVAGEVKDPQRNIPRALIGGTIVIILLYLFINAAYFYVLSPT
                     AVASVSANSSVAAEVTNTFMAPFSASAKALVAGLVAAALMASSVGTLHTSVLTGARVP
                     YAMARDGLFFDKLSKISERTRVPARAVLAQGVWACALVLILQRFDKLTDYVVFGSYIF
                     YGLVTASIFVFRRRLPHAERPYRAWGYPVVPALFLIVTAFLLFMTFWAAPSEAILGLV
                     LIGLGFPVYYFFFRNRLPQHSDAADEDEQT"
BASE COUNT          630 a         1446 c         1157 g          658 t
ORIGIN      
        1 ccggcgaagt gcgggcagcc catctcggcg cgcggggcgg cgtcagggcg cacgacgaac
       61 tccgtgttgc tcatcccgta aggattctcg acgctcatcc agccgatgga gcgcggccgc
      121 gtgcgcgcga gcaattcgcg gctcaggtag acgaagccgg agccgtagcc cgagagcatc
      181 cacttgtggc cggtcgtgca gagggcgtcg atgcccatgc gcttcacgtc gatgtggaag
      241 gcgccggccg actgggcggc gttgacgacg aacgggtggc cgccctttcg tcgcccgatc
      301 tcttcgaggt cggcgcggaa gccgttcgag tattggacgt ggctgagaca gatgaccgag
      361 tcgccttcgc ccgccgcgtc gagcaagtcg tccgtcagca gctcgccatt aacagcctta
      421 actaagcgga cgcgcgcgcc cctgtgcatc cacgtgatgg tggagacggg aaactccaac
      481 tcgccggcga cgacctcgcc gcggcttccg agggcgtcga tgatgaggtt catccccgaa
      541 gaagtgttgg tcgtgaaagc gacctcttcg gggtcggcat tgatgagccg cgcgacgctc
      601 gcccgcgcct gctcgcgacg ctcaagccac tcctcccaga gcttgtcgcc gccctccagc
      661 atctccgcgt agaagccggt cgcggcccga tgcaccgggc gcggcaccgg ccccgacgcg
      721 gcgctgttca gatagactac gttctccatc acggggaaat ccccacgcac tgagcgccat
      781 cccaactcct catccgtgcc cacgatattc tctgccacca tcacgcttcc ctttcacact
      841 tgacgctcag cgccgtctct gtctatcttc gcacgcactc gtaaaacttc cgtcccaacg
      901 ttagaggagc gcccgacccg atgacgaccg aaaccgctga gcgcgcgccc gcctggagta
      961 tccccggctt tctggagaca cgcgaggggc gtctcgcaat caacggcgtg gacgccctcg
     1021 acctcacggc ccgacacgac acgccgcttt tcgtcttctc cgagccgcgc atccgcgcca
     1081 acatcgcgcg cctgcgcgaa gccgccgagc gcgtcggcca ccgcgtcaaa tactgttacg
     1141 cctcgaaggc caactcgaac atggccgtcc tcgccgccgt gcgcgacgcc ggcatcgatg
     1201 tcgaggttaa cagcggcgga gagcttttta aagccctgcg cgccggcttc cgtcccgacc
     1261 agatcatctt caacggcacc agcaaatccg acgccgaact cgacgaggcc gtcggcgccg
     1321 gcatctactc gaccaacgtc gattccgtct acgaaatcga actcgtggaa gccgccgccc
     1381 gccgcacggg caagcgcgcg cgcatcgcgc tccggctcgt ccccgagatc ggcacgcgct
     1441 cacacctcgg cctccagacc gccctgctca cgtcgaagtt cggcatctcg tcatccgaag
     1501 tcctcgacgc cttccgccgc gccctgcgtc accccgaact cgtccacgtc agcggcatcc
     1561 acatccacgt cggctcgcag acgcccgacg tggaacccta cgccgcggcc ttcggggcca
     1621 tgtgggaaca cctcgcgacc atccaccgcg agtcgggcca cgtgctcgaa cacatcaacc
     1681 tcggcggcgg catccccgtc aactatctcc gcgacccgtc gcaggccggc gacctccccg
     1741 agcacgaacg cgacatgctc ggcgccgaac tcgaaccggc ggacgtgctc gcggcggcgc
     1801 ttcaggcggc gcgcgactcc gcccgcgcgg ccggggccga acacctgctc gaaagcgtgg
     1861 agatactcct tgagccgggc cgcagcgtca tcgccgacgc cgccgtgctc ctgaccactg
     1921 ttcgcaacgt caagcaccgc cccgagaccg gcgacgtgtg gctgctcacc gacgccggct
     1981 acaacctcgc gctctcgcag agcatgtaca agtggtacta ccacctcatc tctgcctcgc
     2041 gcgccgccgc gccccacgac accgcctaca aggtggccgg ccccctgtgc gactccggcg
     2101 atgtctactt cgacatcgag cgcgggggac gcctgcccga ttaccgcaag ctcccggccg
     2161 acgtgcggcc cggcgaagtg ctcgcgctct taaacagcgg cgcctacacg ctcgcacaga
     2221 tgaccactta caacgggcgg ccgctcccgg cggccgtcat ggtgcgcgcc ggtgggcgcg
     2281 ttgacgccgc ccgccggcgc gacacatacg aagacctgct cgcgggcgaa gtcttttaaa
     2341 aatattcact gagcctacac gcgtttcaaa tcgcgtgtgc gaccgaccga ccagccgatg
     2401 tcacagacac aacagaatca gacgctcacg ctcgtccgcg gcctcaccct tgtcgccgcg
     2461 atctcgatca tcatcggcaa cgtcatcggc acgggcgtct ttctcaaggc gcgcgtgatg
     2521 acttgcaatg tcggctcgcc cggcatggtg atgaccgtgt gggtcgtcgc cgggctgctc
     2581 agtctcgccg gcgcgctcac ctacgccgaa ctcacggcga tgatgccgcg cgccggcggc
     2641 gagtacgtct tcatgcgcga ggcatatggg ccgggcgccg gcttcctcag cggctggatg
     2701 caaatcctga ttgccaagac cggctcgcag gcttcggtcg cgctcatctt cgccgcctcc
     2761 ctcaacgacc tcacgggcaa ggccgtggaa agcgcctcgg cctacggcgt gcaactgacg
     2821 gcgctcgccg tgatcatcgc catcaccttg ctcaactgcg ccgccgtcaa ggtgagtggc
     2881 tgggtggcga ccgtcctcac cggcgtgaag atcgcgctgg tgctcggcat cggcctcggc
     2941 gcttttttcc tcgcgagcgg cgactggggc aactacgccc tgagcaatct gggcgggacg
     3001 tgtgagggcg tgagtccggg cgcgacgctc ggcgcggcgg gcttcaccgg cgcgatgctg
     3061 ggcgctctgt ggggctacga cgggtggaac aacctgacgt tcgtcgccgg agaggtcaaa
     3121 gacccgcaac gcaacatccc gcgcgcgctc atcggcggca ccatcgtcat catcctgctc
     3181 tacctcttta tcaacgccgc ctacttctac gtcctgtcgc cgacggccgt cgccagcgtc
     3241 tcggccaact catcggtcgc ggccgaggtg acgaacacgt tcatggcccc cttcagcgcc
     3301 agcgcgaagg ccctcgtcgc cgggctggtg gccgcggcgc tgatggcctc ctccgtcggc
     3361 acgctccaca cctccgtgct cacgggcgcc cgcgtcccct acgcgatggc gcgcgacgga
     3421 ctcttcttcg acaagctgtc gaagatttcc gagcggacgc gcgtcccggc gcgcgccgtg
     3481 ctcgcgcaag gggtctgggc ctgcgccctc gtcctcattc tccaacgctt cgacaagctg
     3541 accgactacg tcgtcttcgg ctcctacatc ttctacggac tcgtcaccgc ctcgatcttc
     3601 gtcttccgcc gccgcctgcc gcacgccgag cgcccctacc gggcatgggg ctaccccgta
     3661 gtccccgctc tcttcctcat cgtcacggcc ttcctgctct ttatgacctt ctgggccgcc
     3721 ccctccgaag cgatcctcgg cctcgttctc atcgggctgg ggttcccggt ctactacttc
     3781 ttcttccgca acaggctccc acagcatagt gacgccgcgg atgaagatga acagacttga
     3841 gggggccagc acacgtgaca gtaccgcgag cggtagcgag cgggtctgct t
//
LOCUS       DASVES010000230         3668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_507873, whole genome shotgun sequence.
ACCESSION   DASVES010000230 DASVES010000000
VERSION     DASVES010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507873"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VF240_09395"
     CDS             complement(<1..939)
                     /locus_tag="VF240_09395"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7175459.1"
                     /translation="MKSFYVTTPIYYANSLPHLGHLYTTVVADTLTRHMRQRGYETYF
                     LTGTDEHGTNIERSAARNGRTPREHVDYIVGELKKTFAAFGLDGAHGGYDIFMRTTEP
                     FHYEAVGALWRRVAQARTPMGRECIYKDFYEGWFCAACAEFKTEEQFAPPESEGAPPR
                     CPIHDTPLDRVSEEGYFFRLSDYGDALIELYESRTDFVRPESRRNEVMSFVRAGLQDL
                     SISRLKTSVSWAIPVPDDPLHTVYIWFDALSNYITALGWGNDEQRRADLFDKFWPGTH
                     FIAKDILRQHAIYWPAFLMAAGVELPRGVVCHGLWVV"
     gene            complement(1020..1646)
                     /gene="folE"
                     /locus_tag="VF240_09400"
     CDS             complement(1020..1646)
                     /gene="folE"
                     /locus_tag="VF240_09400"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX7175460.1"
                     /translation="MSTKFSATEPDEESVGSHGAGVDLEKIERGVRLILEGVGEDPDR
                     PGLRRTPQRVAEMYAELTAGMREDPNEHVRPLPGDKHDEMVIVKDIHFASVCEHHLAP
                     FVGRSHIAYIPKGGRIVGLSKLARLIDTFARRLQVQERLTSEIADTIFNVLKPVGVMV
                     VMEAEHTCMTVRGVKKPGAMTVTSAIRGGFRKDPRTRAEAMALIKGDK"
     gene            complement(1684..2871)
                     /gene="larC"
                     /locus_tag="VF240_09405"
     CDS             complement(1684..2871)
                     /gene="larC"
                     /locus_tag="VF240_09405"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX7175461.1"
                     /translation="MRTLYFDCFAGVSGDMTLGALVGVGADERALVEQLGLLGVGGFE
                     VNFDRVERAGLSAVRANVRVEHDPHNHRHLSNILKIIDASALPAGVKERAANIFQRLG
                     EAEARVHNVPVERIHFHEVGALDAIVDVVGACIGFELLGIERFVSSPLHVGSGAVEME
                     HGRFPVPPPAVAELLRGAPIYSTDIVGELVTPTGAAIVSTVCKEFGPLPPMRVVGTGY
                     GAGKREYEKFPNVLRVIVGETEVATASDEKLLMIETNIDDASPQILGHLMETVFTRGA
                     LDCYFTPVQMKKNRPGVLVSILCRPRDREALFELLFSETTTLGARSYEVDRRALERET
                     VRVETEYGPVDVKVGRMGGRVVGGMPEYEQCRAAATRAGVALRDVEAAAVAAFNSWRK
                     ESA"
     gene            complement(2895..>3668)
                     /locus_tag="VF240_09410"
     CDS             complement(2895..>3668)
                     /locus_tag="VF240_09410"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX7175462.1"
                     /translation="APRSATIGVASMPDKSLISGRLRERLRSAREVFVLTGAGVSAES
                     GVPTFRGGGHSAVWKGMPFDVISSAAMIERDLRAVWEWFDYRRELLQTLRPNAAHETL
                     ARWQDTFPALTLATQNVDGLHSAAGSRDVLELHGSVWTARCLACDAKEDLRKLTPEQR
                     PPICGACGGEMRPDVVLFGEMLPVEVFAEAHRAASRCDVCFVVGTSAVVYPAAGLPLV
                     AREAGAYVVEVNPERTALSDVCDETIEGPAGEILPLMDF"
BASE COUNT          650 a         1197 c         1183 g          638 t
ORIGIN      
        1 cacgacccac aggccgtggc agacgacgcc gcgcggcagc tccacgccgg ccgccatgag
       61 gaacgccggc cagtagatcg cgtgctggcg cagaatatct ttggcgatga agtgcgtgcc
      121 cggccagaac ttgtcgaaga gatcggcgcg gcgctgctcg tcgttgcccc atccgagcgc
      181 cgtgatgtag ttcgagagcg cgtcgaacca aatgtagacg gtgtgcagcg ggtcgtcggg
      241 gacggggatg gcccaactca cagaagtttt caagcggctg attgataagt cctgaaggcc
      301 ggcgcgaacg aagctcataa cttcgttgcg tcgcgactcg gggcggacga agtcggtacg
      361 actctcatac aactcaatga gcgcgtcgcc gtaatcggag aggcggaaaa agtagccttc
      421 ctcggagacg cggtcgagcg gcgtgtcgtg gatggggcaa cgcggcggcg cgccctccga
      481 ctcggggggt gcgaactgct cctcggtctt gaactcggcg cacgcggcgc agaaccagcc
      541 ctcgtaaaaa tctttgtaga tgcactcgcg ccccatcggc gtgcgcgcct gcgccacccg
      601 tcgccataac gcgcccacgg cctcgtagtg gaaaggctcg gtggtccgca tgaagatgtc
      661 gtagccgccg tgagcgccgt cgagtccgaa ggcggcgaag gtcttcttca actcgccgac
      721 gatgtaatcg acgtgctcgc gcggcgtgcg cccgttgcgg gcggcgctgc gctcgatgtt
      781 ggtgccgtgc tcgtccgtgc ccgtgagaaa gtaggtctcg tagccgcgct gccgcatgtg
      841 ccgcgtgagc gtgtccgcca cgacggtcgt gtagagatgc cccaagtgcg ggaggctgtt
      901 ggcgtaatag atcggtgtgg tgacgtagaa agatttcata aaaaccgtga taagtgatga
      961 gtgataagtg ataagaaaag caaaacgcgt ctgtcttctt tctcatcact taacactcat
     1021 cacttatcac cctttataag tgccatcgcc tcggcgcggg tgcgggggtc tttgcggaag
     1081 ccgccgcgga tggccgaggt gacggtcatc gcgcccggct tcttcacgcc gcgcacggtc
     1141 atgcacgtgt gctcggcctc cattaccacc attacgccga ccggcttgag cacgttgaag
     1201 atggtgtcgg cgatctcgga ggtcagacgc tcctgcacct ggaggcggcg ggcgaaggtg
     1261 tcgatgagcc gcgccagctt cgagaggccg acgatgcggc cgcccttcgg gatataggcg
     1321 atgtggcttc ggccgacgaa gggcgcgagg tggtgctcgc agacggaggc gaaatggatg
     1381 tctttgacga tcaccatctc gtcgtgcttg tcgccgggca gcgggcggac gtgctcattg
     1441 gggtcttcgc gcatccccgc ggtcagctcg gcgtacatct ccgcgacgcg ctgcggcgtg
     1501 cgccgcagtc cgggccggtc ggggtcttcg cccacgcctt cgaggatgag acgcaccccg
     1561 cgctcgatct tttcgagatc gaccccggcg ccgtgcgacc cgaccgactc ctcgtccggt
     1621 tcggtggcgg aaaactttgt ggacatctcg acgcctctct tacacgcgcc cgaaggcgac
     1681 gcactacgct gactctttgc gccaactgtt gaaggccgca acggcggccg cttccacgtc
     1741 gcgtagggca acaccggccc tcgtggcggc cgcgcggcat tgctcgtact ccggcatccc
     1801 gccgacgacg cgcccgccca tgcgccccac cttcacgtct accgggccgt actcagtctc
     1861 cacacgcacg gtctcgcgtt ccagcgcccg ccggtcaact tcgtaactgc gtgcgccgag
     1921 cgtcgtcgtc tcactgaaca ataattcaaa cagcgcctcg cggtcacgcg gccggcacag
     1981 gattgagacg aggacgccgg gccggttctt tttcatctgg acgggcgtga agtagcaatc
     2041 gagcgcgccg cgtgtgaaga cggtctccat caggtgaccg agaatctgcg gcgacgcgtc
     2101 gtcgatgttc gtctcgatca tcaacagctt ctcatcgctg gcggttgcta cttcagtctc
     2161 acccacgatg actcgcagta cgtttgggaa tttttcgtac tcgcgcttcc ccgccccgta
     2221 gcctgtcccc acgacccgca tcggcggcag cgggccgaac tctttgcaga cggtgctgac
     2281 gatggccgcg ccggtcggcg tgacgagttc gccgacgatg tcggttgagt agatgggtgc
     2341 gccgcggagc agttcggcca cggccggcgg cgggacggga aagcgcccgt gctccatctc
     2401 gacggcgccg ctcccgacgt gtagcggcga cgagacgaaa cgctctatgc cgaggagttc
     2461 aaacccgatg catgcgccga ccacgtctac aatcgcgtcc aaagcgccga cctcgtggaa
     2521 gtgtatccgc tcgacgggga cgttgtggac gcgcgcctcc gcctcgccga gtctttgaaa
     2581 aatgttcgct gcgcgctcct tcacgccggc cggcagggcg gaggcgtcga tgatcttcaa
     2641 aatgttcgag aggtgccggt gattgtgcgg gtcgtgctcg acccgcacgt tcgcacggac
     2701 ggcgctcagc cccgcacgtt cgacacggtc gaagttgacc tcgaagccgc cgacgccgag
     2761 gaggccgagt tgctcgacga gtgcgcgctc gtcggcaccg acgccgacga gcgccccgag
     2821 cgtcatgtcg ccgctcaccc ccgcgaagca atcgaaataa agtgtccgca tcagaagtcg
     2881 atcagtggaa gaaatcaaaa gtccatcagt ggaagtatct cgcccgccgg cccctcaata
     2941 gtttcgtcgc acacgtcgga gagcgccgtc cgttccgggt tgacttcaac gacgtaagcg
     3001 cccgcctcgc gcgcgacgag cggcagaccc gccgccgggt agacgaccgc cgaagtcccc
     3061 acgacgaaac acacgtcgca gcgagaggcg gcccggtgtg cctccgcgaa aacttccacc
     3121 ggcagcatct cgccgaagag caccacgtcc ggcctcatct cgccgccgca cgcgccgcag
     3181 atcgggggcc gctgctccgg cgtgagcttc cgcaaatctt ctttcgcgtc gcacgcgaga
     3241 cagcgcgccg tccagacgct cccgtgcagt tccagcacgt cgcgcgagcc ggcggcgctg
     3301 tgtaagccgt caacgttttg cgtggcgagg gtgagcgccg ggaacgtgtc ctgccagcgc
     3361 gcgagagttt cgtgcgccgc attcggacga agcgtctgaa gcagttcgcg ccggtagtcg
     3421 aaccattccc acacggcgcg caggtcgcgc tcgatcatcg cggccgagga gataacgtcg
     3481 aacggcattc ccttccagac ggccgaatgg ccgccgccgc gaaaggtcgg caccccgctt
     3541 tccgcggaga cgcccgcgcc cgtgagcacg aagacctccc gcgcgctgcg gagacgttca
     3601 cgcaaacggc ccgaaatgag cgacttatcc ggcatcgaag cgacaccgat ggtagcagag
     3661 cgcggcgc
//
LOCUS       DASVES010000231         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_392153, whole genome shotgun sequence.
ACCESSION   DASVES010000231 DASVES010000000
VERSION     DASVES010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392153"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1009)
                     /locus_tag="VF240_09415"
     CDS             complement(92..1009)
                     /locus_tag="VF240_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7175463.1"
                     /translation="MRDIVGRKNLTSKEAARLLGVSEASVKRWADGGLLPALKTAGGH
                     RRFRPEDVAAFRRDGLTRETASAFRRNGAPESITRASHAAAALDETLPQMMFETIVGG
                     HDEEAGAALVSLYLQGHTVARIADAVLCPALRKVGDLWHAGELSVAQEHVATRTALSS
                     LQALRASLNVREREGARTAVCCSVEDDFHEMPVQLAVLTLEALGWGVVNLGTSTPFYA
                     LSEAVARFSPRVVCVASTVFNHPDRAAREYGEFSTAAGRVGASVVLGGAGFQAEGVRR
                     RLPADLYAETFSQLESHAAALDAGAVGGD"
     gene            1224..1769
                     /gene="idi"
                     /locus_tag="VF240_09420"
     CDS             1224..1769
                     /gene="idi"
                     /locus_tag="VF240_09420"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX7175464.1"
                     /translation="MPTDEQVILVDERDRELGAGEKLRAHREGALHRAFSVFIFDRGG
                     RLLIQRRAREKYHSAGLWSNTACGHPRPGEATKEAARRRLREEMGLDCELRAAFEFLY
                     RAELDGALVEHEYDHVFVGTHEGESPPAPDPSEVEDWRWVGMDELRRGLRHEPHLYSY
                     WLKLAVESGHWRRLDALVVGS"
     gene            1789..2511
                     /locus_tag="VF240_09425"
     CDS             1789..2511
                     /locus_tag="VF240_09425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7175465.1"
                     /translation="MSEQSGSVVLILGAGRGIGRAAAHLFAQGGDALIIASRNETDLE
                     SLRRELSENYGARVLAVPTDATDAGAVEHLVDAALNAFGRVDTAVYAAGVGVLKPFAE
                     TTAGEFEELMRTNVGGAFNLCKAVLPVMERQKGGRLVAIPGILGRAPMAQAAAYCASK
                     YALTGMLKSLALETKRAGVRVSLLHLGGVNSSFWDNITMRVQRERMLTVGAAARAVHF
                     AATQEGEGVLNELILQPESHQL"
     gene            2546..3052
                     /locus_tag="VF240_09430"
     CDS             2546..3052
                     /locus_tag="VF240_09430"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7175466.1"
                     /translation="MTKEMTQEDKLGKLREIVKAVDICMLTTIDERGDLHSRPMSNNG
                     DIEFDGDLWFFTYGASHKVDEVGRVPKVNASFADTDAQQYASLTGRAEVVRDRAKIEE
                     LWKPQLRAWFPEGVETPDIALLKMTVERAEYWDGSQSMVAHAVSLVSSLVTGKPAQLG
                     ENEKLELK"
     gene            3049..>3367
                     /locus_tag="VF240_09435"
     CDS             3049..>3367
                     /locus_tag="VF240_09435"
                     /inference="COORDINATES: protein motif:HMM:NF025686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 domain-containing protein"
                     /protein_id="HEX7175467.1"
                     /translation="MKFDYALDFARTNFREHPELYRVGRGEQGVLLVEPYKGEILPHW
                     RFRTPDVARESAAKIHELYEGYKSAGDFVGMDMARKYLQMGWTRSMRYANHRTGKKYV
                     GPVP"
BASE COUNT          609 a         1037 c         1159 g          562 t
ORIGIN      
        1 tcgacaaatc tttttgctat ctgctccgac atcttcaaaa ccccctttgt gcgtgtgggc
       61 cgccggcgcg cggccgcgcg acgatgtttg attagtctcc gccgacggcg cccgcatcga
      121 gcgccgccgc gtgactctcc agttgagaga atgtttccgc gtacagatca gcagggagcc
      181 tgcgccgcac gccctcggcc tgaaaacccg cgccgcccaa aacgaccgac gccccaacgc
      241 gtcccgccgc tgtgctgaac tcgccgtact cgcgcgccgc ccggtcgggg tggttgaaca
      301 ccgtcgaggc gacgcagacc acgcgcgggc tgaaacgcgc gaccgcctcg gagagcgcgt
      361 agaacggagt gctcgtgccg agattgacga cgccccaccc tagcgcctcc agcgtcagca
      421 cggcgagctg gacgggcatc tcgtggaagt cgtcctcgac gctgcaacag acggccgtgc
      481 gcgcgccctc ccgctctctc acgttcagcg acgcgcgcag tgcctgtagc gacgagagcg
      541 ccgtgcgcgt cgccacatgc tcctgcgcca ccgacaactc gccggcgtgc cagaggtcgc
      601 cgaccttacg tagcgcgggg cagagcaccg cgtccgcgat gcgcgccacc gtgtgccctt
      661 gcaggtagag gctgacgagc gcggccccgg cctcctcgtc gtgcccgccg acgattgtct
      721 caaacatcat ttggggcagg gtttcatcga gtgcggcggc ggcgtgcgac gcgcgggtaa
      781 tgctttcggg tgcgccgttt ctcctgaaag cactcgcggt ctcgcgcgtc aggccgtcgc
      841 ggcggaaggc ggccacgtcc tcggggcgga agcgcctgtg cccgcccgcg gtcttcaagg
      901 ccggcagcag accgccgtcg gcccaacgct tgaccgacgc ctcgctcacg ccgagcagcc
      961 gcgccgcctc cttgctggtc aaattttttc gcccgacgat gtctctcatg tccgccggcc
     1021 aatctagcag agctttcggc ggatgtaaat aaaaatcgtt caatccgagc attcaagctc
     1081 aattcgtgac acaataagca cggaatctta acttgacttt agatgtatgt tgattaaaat
     1141 cgtgcaaatc gctcaagggc gagagtgtgt aagaaaatat tctttgcggt cttcaaaggg
     1201 cctaaacgcg ggtggaacta cggatgccga cggacgaaca ggtgattctc gttgacgagc
     1261 gcgaccgcga gctgggggcg ggcgagaaac tgcgcgcgca cagagagggc gcgctgcaca
     1321 gggcattctc cgtcttcatc ttcgaccgcg gcggtcggct gcttattcag aggcgcgcgc
     1381 gggagaagta tcactccgcg gggctgtggt cgaacacggc ctgcggccac ccgcgccccg
     1441 gtgaggcgac caaagaagcg gcgcggcgga gattgcgtga ggagatgggc ctcgactgcg
     1501 agctgcgcgc ggcgttcgag tttctctacc gcgcggaact cgacggcgcg ctcgtcgagc
     1561 acgagtacga ccacgtcttc gtcggcaccc acgaggggga gtctcctcct gcgcccgacc
     1621 cttcggaggt cgaggactgg cgttgggtgg gcatggatga gctgcgccgg ggcttgcgcc
     1681 atgagccgca cctttatagc tactggctga agctcgccgt cgagagcggc cactggcgca
     1741 ggctcgacgc gctcgtggtc ggcagttgaa tcagggaggg tggggaagat gtcggagcag
     1801 agcgggagcg tggtgttaat tctgggagcc gggcgcggca tcgggcgcgc ggcggcgcac
     1861 ctgttcgcac agggcggcga cgcgctcatc atcgcctcgc gcaatgagac cgatctcgaa
     1921 agtctgcgac gcgaactctc ggaaaactac ggcgcgcgcg tgctcgccgt gccgaccgac
     1981 gcgacggacg cgggggcggt cgaacatctg gttgacgccg cgctcaacgc tttcgggcgc
     2041 gtggacacgg ccgtctacgc ggcgggcgtc ggcgtgctca agcccttcgc cgagacgacc
     2101 gccggggagt tcgaggaatt gatgcggacg aacgtgggcg gggccttcaa tctctgcaag
     2161 gcggtgttgc cggtcatgga acggcagaag ggcgggcggc tcgtcgccat ccccggcatc
     2221 ctcgggcgcg cgccgatggc gcaggccgcc gcttattgcg cctcgaagta cgcgctcacg
     2281 gggatgttga agtcgctcgc gctggagacg aagcgcgcgg gcgtgcgcgt gagtctcctg
     2341 cacctcgggg gcgtcaactc gtccttctgg gacaacatca cgatgcgcgt ccagcgggag
     2401 cgcatgttaa cggtgggcgc ggcggcgcgg gccgttcact tcgccgcgac gcaggagggc
     2461 gaaggagttt tgaacgaatt gatcttgcag ccggagtcgc atcagttgtg agacggggac
     2521 gcattcaaac gggaggaatt tgaagatgac taaagagatg acgcaagagg acaagctcgg
     2581 gaagttgcgg gagatagtca aggccgtgga catctgcatg ctgaccacga ttgacgagcg
     2641 gggcgacctg cacagccgac cgatgtcgaa caacggcgac atagagttcg acggcgacct
     2701 gtggttcttc acctacggcg cttcgcacaa ggtggacgag gtcgggcgcg tgccgaaggt
     2761 gaacgccagc ttcgcggaca cggacgcaca gcaatacgcg tcgctcacag ggcgggccga
     2821 ggtcgtgcgc gaccgcgcga agatcgagga gctgtggaag ccgcaactcc gcgcgtggtt
     2881 ccccgaaggg gtcgagacgc cggacatcgc gctgctcaag atgacggtcg agcgtgcgga
     2941 atactgggac ggttctcagt caatggtggc ccacgccgtc agcctcgtct cctcgctcgt
     3001 cacgggcaag ccggcgcagt tgggtgaaaa cgagaagctc gaattgaaat gaagttcgac
     3061 tacgcgctcg acttcgcccg gacgaatttc cgcgagcacc cggagttgta ccgtgtcggg
     3121 cgcggcgagc agggcgtgct cctcgtcgag ccgtacaagg gggagattct tcctcactgg
     3181 cgcttcagga cgccggacgt ggcgcgggag tcagccgcga aaatccacga gctgtacgaa
     3241 ggctacaagt cggcgggcga tttcgtcggg atggacatgg cgcgcaagta tctccagatg
     3301 ggatggacgc gctcgatgcg ctatgccaac caccggacgg ggaagaagta tgtggggcct
     3361 gtgccgc
//
LOCUS       DASVES010000232         8241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1612028, whole genome shotgun sequence.
ACCESSION   DASVES010000232 DASVES010000000
VERSION     DASVES010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612028"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /gene="holA"
                     /locus_tag="VF240_09440"
     CDS             complement(<1..935)
                     /gene="holA"
                     /locus_tag="VF240_09440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7175468.1"
                     /translation="MSAATREELKRQLKAGQVAPLYLLHGPEDYLRGRAARAISDAAL
                     SGAPLREFNESSFSLTSTDVQQAIASAEQLPMMSSRRVVRVTDFGRLRDADEEALLRY
                     LDRPAESSTVVFIADDLDKRRKLSKVLLQACVSVEFAELKDAEAVAWARSRLRELKAE
                     ADERTLRQIVGLVGTGVRRLESELTKLATAALPGSLISMDMVEALVGRSREMSNFELT
                     DHLIARNRSRALETLRRLLDDGAEPVMLIGLLASSYHRLALAKDLMSRGAPKEEVFRL
                     VPMPYSMREDFLATARRADADELATRIRRIADADLR"
     gene            complement(932..1489)
                     /gene="lptE"
                     /locus_tag="VF240_09445"
     CDS             complement(932..1489)
                     /gene="lptE"
                     /locus_tag="VF240_09445"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX7175469.1"
                     /translation="MLSIRGLFRAAFVASAALCLTGFTECYKPVKKSGLPSHVRTVAV
                     PAFQNEARRYKIESRFTDAVINEVVKRGRGLRVQSEREGADIVIDGVVKDFGFYGVLL
                     DNRGRSRIFEVRVVAAVTVRDRVHNRVLYDNQNYEFRGEFEFTSDPRTFFNEEEPAVQ
                     RLARAFAESLVATLVDGFGVNEGRP"
     gene            complement(1671..2534)
                     /gene="folP"
                     /locus_tag="VF240_09450"
     CDS             complement(1671..2534)
                     /gene="folP"
                     /locus_tag="VF240_09450"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7175470.1"
                     /translation="METRLWKLARRSLPVGGRTLVMGVLNVTPDSFSDGGLFYDFGRA
                     VERAEEMTAEGADIIDVGGESTRPGGAPVSVEDESRRVVPVIEAVAGVAGVPVSVDTT
                     RAQVARAALDAGAEIINDISGLRFEPELADEAARAGAGLVLMHSRGERETLHALEPVE
                     DIMDEVVASLRRSVAEAVGRGVRSESIVIDPGVGFGKSQEQNLELIARLDEFAAALPE
                     FPVLVGTSRKSFIGRITGGAPAGERLHGTMATVTAAILRGAHIVRVHDVRAAVETARV
                     AEEIRAVNRQS"
     gene            complement(2653..3417)
                     /locus_tag="VF240_09455"
     CDS             complement(2653..3417)
                     /locus_tag="VF240_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX7175471.1"
                     /translation="MIVALTREPARAMSGCELTYLERQGIDAALAAEQHRAYRETLAS
                     CGARVVALPAIEELPDSVFVEDTAIVLDELVVLTSPGNESRRGEVGLIEPEVARLRTV
                     ERVSLPATIEGGDVLRAGRRLFVGLSPRTNHAGAEALRRLASPFGYEVRAVEVRDCLH
                     LKTGCSALDDETILVNPDWVDTDAFRDYEVVRVHAAEPWAANALRVGASVCVSAAHPR
                     TAELIGRRGYAVRPLDISEFAKAEGGLTCLSLIFKQ"
     gene            complement(3533..5470)
                     /gene="ftsH"
                     /locus_tag="VF240_09460"
     CDS             complement(3533..5470)
                     /gene="ftsH"
                     /locus_tag="VF240_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7175472.1"
                     /translation="MSSTARQLIFWVLILVGAVMLYQFLVNKQGRASQDIDITALREK
                     IEKRQVEKLTFRQGEVVAVDAAKNEFKTKLSNDDYRSKLMEFSGEKIEGGTRVKEIID
                     EPASSGLLWSVLIWWAPILLLIGFWIFMLRQMQSGGNKALSFGKSRAKLLNNQQKRVT
                     FKDVAGVEEAKEELQEIIEFLKEPQKFQKLGGRIPKGVLMMGPPGTGKTLLARAVAGE
                     ANVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGG
                     GHDEREQTLNQLLVEMDGFESNDGVILMASTNRPDVLDPALLRPGRFDRRVVVSRPDV
                     RGREGILKVHTRKIPLGDDVDISIIARGTPGFTGADLANLVNEAALNAARYNKKIVMQ
                     SDFELAKDKVLMGAERKSMVITNEEKRVTAYHEAGHTLVGLKVPNADPVHKVTIIPRG
                     MALGVTQQLPEGDRHNYTKEYLLGQIAILMGGRIAEEKFLGNITTGASNDIERATELA
                     RAMVCEYGMSDLGPLTFGKKEEQIFLGREIAQHRDYSEDTAIKIDNQVKYIVNEQYGR
                     ATTIIEDNRDTMVRLAETLLERETLDGVQIRRIVAGLSLDDDSGAPQSKDEDRPQLKE
                     PAANPLKPILPPITGSNPATA"
     gene            complement(5719..6231)
                     /locus_tag="VF240_09465"
     CDS             complement(5719..6231)
                     /locus_tag="VF240_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7175473.1"
                     /translation="MDQKIVEIYGAAEIEGRLKALADEISEGSRADELTVLGVTDDGF
                     VFLADLLRALSVPVRTALLRYDHRARGGAEDLTFSTNIDLKGRDVLLVEGVLDTGVPQ
                     EYLMKQLESQGARRVRLCVLIDKPDGRRTPVKPDWRAFETHEDYLVGYGLGFQERWRE
                     LPYIATFARG"
     gene            complement(6364..7557)
                     /gene="tilS"
                     /locus_tag="VF240_09470"
     CDS             complement(6364..7557)
                     /gene="tilS"
                     /locus_tag="VF240_09470"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7175474.1"
                     /translation="MKTATEKRARGVGRTGRRGLSDFAGRLLDEWERRGWPSDAAHVV
                     VAVSGGADSAALLLALDELTRAGRVSLCVIVAHLDHGLRGERGEADAVWVAAQASALG
                     LAAVVGRADVEARARARKDNLEQAARGARYEFLGDVARAHGACAVLTAHTLDDQAETI
                     LLRLLRGSGAGGLAGILPERELSERMTIENRASDATAGEGVRLLRPLVAWARRADTVG
                     YCRERGVEFRADEMNEDERFARVRVRRRLLPLLETFNPRAVEALARTAELLGEDASAL
                     EAEAARLLEDATPESESEETSAAGGAWPLRVEALASAARAVRRRALRQWIARGRGDLR
                     RVEHVHVLAVEKLLEGTRGGRVAELPGGCRIERRRGWLCFRDGSAEDARNGAAGERGT
                     GRGRK"
     gene            7793..>8241
                     /locus_tag="VF240_09475"
     CDS             7793..>8241
                     /locus_tag="VF240_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175475.1"
                     /translation="MEQRKDTRAARLRRDTSTKALRLIVALVLPAMLGLVSTPHAAHA
                     AMSADDASAAAAVVTNTLGVQERFEGQWLIDFTQRADGRVQLTLRYRNEKGTKGDSDG
                     WNWNDSFGIDASQLHGLARETAMSAAGTSVRFQIKRDAGAIDCEGWFR"
BASE COUNT         1365 a         2859 c         2614 g         1403 t
ORIGIN      
        1 cggaggtcgg cgtcggcgat gcggcggatg cgtgtggcga gttcgtcggc gtcggcgcgg
       61 cgggcggtgg cgaggaagtc ctcgcgcatc gagtagggca tgggcacgag gcggaagacc
      121 tcctccttcg gcgcgccgcg cgacatcaaa tctttggcga gcgcgagccg gtgatagctg
      181 ctcgcgagca ggccgataag catgaccggc tcggccccgt cgtcgagcag tcggcgcagg
      241 gtctccagag cgcggctgcg gttgcgcgcg atgagatggt cggtcagctc gaagttggac
      301 atctcgcgcg aacgacccac gagcgcctcg accatgtcca ttgagataag actgccgggc
      361 agcgccgccg tcgccagctt cgtcagctcc gactccagac gccgcacgcc cgtccccacg
      421 agacccacta tctgccggag cgtgcgctcg tccgcctccg ccttcaactc gcgcagacgc
      481 gacctggccc acgccacggc ctccgcatcc ttcagctccg cgaactcgac gctcacgcac
      541 gcctggagga gcaccttgct cagcttgcgc cgcttgtcga ggtcgtcggc gatgaagacg
      601 acggtggagg attcggcggg gcggtcgagg tagcgcagca atgcttcttc gtcggcgtcg
      661 cgcaggcggc cgaagtccgt cacgcgcacg acgcggcgtg aggacatcat gggcagttgc
      721 tcggcggagg cgatggcctg ctgcacgtcc gtggacgtga ggctgaagct cgactcgttg
      781 aactcgcgca gaggcgcgcc ggagagcgcg gcgtcgctga tggcgcgggc ggcgcgaccg
      841 cgcagatagt cttcggggcc gtggaggagg tagagcgggg cgacctgtcc cgccttcaat
      901 tgacgcttga gttcttcgcg cgtggcggcg ctcatggccg cccctcgttg acgccgaaac
      961 catcgacgag cgtcgccacg aggctctcgg cgaaggcgcg tgcgaggcgc tggacggccg
     1021 gctcctcctc gttgaagaag gtgcgcgggt cggaggtgaa ctcgaactcg ccgcgaaact
     1081 cgtaattctg attgtcgtag agcacgcggt tgtggacgcg gtcgcggacg gtgacggcgg
     1141 cgacgacccg cacctcgaag atgcgcgagc gcccgcggtt gtcgagcagc acgccgtaga
     1201 agccgaagtc tttgacgacg ccgtcgatga cgatgtcggc cccttccctc tcgctctgca
     1261 cgcgcagtcc ccgcccgcgc ttgacgacct cgttgatgac ggcgtccgta aagcgcgact
     1321 cgatcttgta gcgccgcgcc tcgttctgga aggcgggcac ggccacggtg cggacgtggc
     1381 tgggcagccc cgacttcttc accggcttat agcactcggt gaagccggtg aggcagaggg
     1441 ccgcggaggc gacgaacgcc gcgcggaaga gtccgcgaat cgagagcatc ttcaggtttc
     1501 cttccaaaat gctgaggccg tgaatcgtta aagaccgtga atcgtaaatc gtaaatcgtg
     1561 aatcgtcaaa agccgtgaat cgtcaatcgt aaatcgtgaa tcgtgattga aggtcgcggg
     1621 gcgtgagcgg tgaatcgcaa gtcgggtgag ctataaccac gattgacgat tcacgattga
     1681 cgattcacgg cccttatctc ttccgcgacg cgcgccgtct cgaccgccgc gcgcacgtcg
     1741 tggacgcgga cgatgtgcgc gccgcgcagg attgcggcgg tgacggtcgc catcgtgccg
     1801 tgcaggcgct cgccggcggg cgcgccgccc gtgatgcggc cgatgaagga cttccgggac
     1861 gtgccgacga gcacgggaaa ctccggcaac gcggcggcga actcgtccag acgggcgatc
     1921 aactccagat tttgctcctg acttttgccg aagccgacgc cggggtcgat gacgattgac
     1981 tccgaacgga cgccgcggcc cacggcctcc gccacgctcc gacgcagaga ggcgaccact
     2041 tcatccatga tgtcctcgac gggttcgagc gcgtgcagcg tctcgcgctc accccgcgag
     2101 tgcatgagca cgagaccggc cccggcgcgc gcggcctcat cggcgagttc cggctcgaag
     2161 cgcaggcccg agatgtcgtt gattatctca gcccccgcgt cgagcgcggc ccgcgcgacc
     2221 tgcgcccgcg tcgtatcgac ggagacgggg acgcccgcga cgcccgcgac ggcctcgatg
     2281 accggcacga cgcgccgcga ctcgtcctca acagagacgg gcgcgccgcc cggccgcgtc
     2341 gattccccgc ccacgtcgat gatgtcggcg ccctcggccg tcatctcctc ggcgcgctcc
     2401 acggcccggc cgaagtcgta gaacagcccg ccgtcgctga agctgtcggg cgtcacattg
     2461 agcacgccca tcacgagcgt gcgccccccg acgggcagcg agcggcgcgc gagtttccag
     2521 aggcgtgtct ccataattca gagtgagcag tgagcagtaa gcagtgagca gagttttttg
     2581 tagacgttga cgcattcatc ttactgctct gccaggatgg cgtcttactg ctcactgctt
     2641 actgctcact gctcactgct taaagatcag actcaaacaa gtcagcccgc cctcggcctt
     2701 cgcgaactcc gagatgtcga ggggtcgcac ggcgtagccg cgccgcccga tcaactcggc
     2761 ggtgcgcggg tgcgcggcgc tcacgcacac ggaggctccc acgcgcaaag cgttcgcggc
     2821 ccacggctcg gcggcatgga cgcgcacgac ttcgtagtcg cggaaggcgt ccgtatccac
     2881 ccaatcgggg ttgacgagga tcgtctcgtc gtcgagcgcg gagcagccgg tcttgaggtg
     2941 caggcagtcg cgcacctcga cggcgcgcac ctcgtagccg aacggcgagg cgaggcggcg
     3001 gagggcttcg gcgcccgcgt ggtttgtgcg cggcgagagg ccgacgaaga gccggcgccc
     3061 ggcgcgcagc acgtcgccgc cttcaatcgt cgccggcagt gaaactcttt ctaccgtgcg
     3121 gaggcgcgcg acctccggct cgatgaggcc gacctccccg cgccgcgact cgttgccggg
     3181 cgacgtgagg acgaccaact cgtcgagcac gatggccgta tcttcgacga agacgctgtc
     3241 gggaagttct tcgatggccg gcagcgccac aacgcgtgcg ccgcaggacg cgagcgtttc
     3301 gcgatacgcg cggtgctgct ccgcggcgag tgcggcgtca atgccttggc gttcgaggta
     3361 cgtcaactcg cacccgctca tggcccgcgc cggctccctc gtgagtgcga cgatcatcgt
     3421 gaggtcaaag gtaaactaaa gaaggcaaaa gtaaaaaggc aaaaggcaaa agtgaagagg
     3481 cgacgtgcct ttctactttt gccttttgcc tttttacttt tgccttaccc agctacgcag
     3541 tagcggggtt gctgcccgtg atgggcggca ggatcggctt gagcgggttg gcggccggct
     3601 ccttgagctg cggccggtct tcgtccttcg actgcggcgc gccggagtcg tcgtcgagcg
     3661 acagtcccgc cacgatgcgg cgaatctgca cgccgtccag ggtctcgcgc tccagcaacg
     3721 tctcggcgag gcgcaccatc gtgtcgcggt tgtcctcgat gatggtcgtc gcgcgcccgt
     3781 actgctcgtt gacgatgtac ttgacctgat tgtcgatctt gatcgccgtg tcttccgagt
     3841 agtcgcggtg ctgcgcgatc tcgcggccga ggaaaatctg ctcctccttc ttgccgaagg
     3901 tgagcgggcc gaggtcgctc atgccgtact cgcagaccat cgcgcgggcg agttccgtgg
     3961 cgcgctcgat gtcgttcgac gcgccggtcg tgatgttacc gaggaacttc tcctcggcga
     4021 tgcgcccgcc catcagaatc gcgatctggc cgagcaggta ttccttcgta tagttgtggc
     4081 ggtcgccctc agggagttgc tgcgtcacac cgagcgccat gccgcgcggg atgatcgtca
     4141 ccttgtggac ggggtcggcg ttcggaacct tgaggccgac gagcgtgtgg cccgcctcgt
     4201 ggtaggccgt gacgcgcttc tcctcgttgg tgatgaccat tgacttgcgc tcggcgccca
     4261 tcagcacctt gtccttggcc aactcgaagt cgctctgcat gacgatcttc ttgttgtagc
     4321 gcgcggcgtt caatgcggcc tcgttgacga ggttcgctaa atcagcgccg gtgaagccgg
     4381 gcgtgccgcg ggcgatgatg ctgatgtcaa catcgtcgcc gagcggaatc ttgcgcgtgt
     4441 ggactttgag gatgccttcg cgcccgcgca cgtcggggcg ggagacgacg acgcggcggt
     4501 cgaagcggcc gggccgcaaa agcgcagggt cgagcacgtc cggccggttc gtcgaggcca
     4561 tcaggatgac gccgtcgttc gactcgaagc cgtccatctc gacgagcaac tggttaagag
     4621 tctgctcacg ctcgtcgtgc ccgccgccta atccggcgcc acggtgacgg ccgacggcgt
     4681 cgatctcgtc gatgaagatg atgcagggcg cgttcttctt gccctgctcg aagaggtcgc
     4741 ggacgcgcga ggctccgacg ccgacgaaca tctcgacgaa gtcggaaccc gagatggaga
     4801 agaacgggac gttggcctca cccgcgacgg cgcgcgctaa aagcgtcttg cccgtgcccg
     4861 gcggccccat catcaacacg cctttgggga tgcgcccgcc gagcttctga aacttctgcg
     4921 gctccttgag gaactcgatg atctcctgta gctcctcttt ggcctcctcg acgccggcca
     4981 cgtccttaaa cgtgacgcgc ttctgctggt tgttgaggag cttggcgcgc gacttcccga
     5041 atgagagcgc cttgttgccg cccgactgca tctgccgcaa catgaaaatc cagaacccga
     5101 tgagcagaag gatgggcgcc caccagatga gcaccgacca gagcagcccc gacgacgccg
     5161 gctcgtcgat gatctccttg acgcgcgtgc cgccctcgat cttctcgccg gagaactcca
     5221 tcagcttgct gcggtagtcg tcgttcgaca gcttggtctt gaactcgttc ttcgccgcgt
     5281 ccacggcgac cacttcgccc tgccggaacg tcagcttctc gacctgccgc ttctcgatct
     5341 tttcgcgcag cgccgtgatg tcgatgtcct gcgaggcgcg cccctgcttg ttcacgagga
     5401 actgatagag catcaccgcg ccgaccagga tcagcaccca gaaaattagt tgtcttgccg
     5461 tggaactcaa aatttttccg cctccgacgc cgatgataac cgattccggc gtcacttgtc
     5521 gctttcccga agtcccctga aacttgcgcc cgcctcggcc cgcgccggat ggggacgaac
     5581 actgctttca gcagccgacc gccgcgggag caattaagat gcccggggca ctcggtcagt
     5641 tgtgcccgaa gcctgcaaag aacgtggccc agcgcggctc aaccgtcgcg ctccgccggg
     5701 ccgcgggggt tatacgactt aaccccgcgc gaaagttgcg atgtagggca gctcgcgcca
     5761 gcgctcctga aagcccagcc cgtagccgac cagatagtcc tcgtgcgtct cgaaagcgcg
     5821 ccagtcgggc ttgaccgggg tgcgccgccc gtccggcttg tcgatgagca cgcacaggcg
     5881 gacgcggcgc gcgccctgcg actccaactg cttcatcagg tattcctgcg gcacgcccgt
     5941 gtcgagcacg ccctcgacta agagcacgtc gcgccccttg aggtcgatgt tggtgctgaa
     6001 ggtcaaatct tccgccccgc cgcgcgcgcg gtggtcgtag cgcagcagcg cggtgcggac
     6061 ggggacgctc aaagcgcgca gcaggtctgc caaaaagacg aagccgtcgt cggtgacgcc
     6121 gagcacggtg agttcgtcgg cgcgggagcc ttcggagatt tcgtcggcga gcgccttgag
     6181 gcgtccttcg atctcggccg ccccgtaaat ctccacgatt ttttgatcca ttacgctccg
     6241 ccctcgccgc acgcgcttcg tttgacaaca aaaaccgctg gcccgcgggc cggcggttga
     6301 agacatttca gattctaaag gccggggggg gctttttcaa ctactttcgc cccggctcct
     6361 caactacttt cgcccccggc cggttccgcg ctccccggcc gccccattac gcgcgtcttc
     6421 cgccgacccg tcgcggaaac agagccagcc ccgccgccgc tcgattcgac agccccccgg
     6481 caactccgcc acgcgccccc cgcgcgtccc ctccaacagt ttctcgaccg ccagcacgtg
     6541 tacgtgttcc acgcgtctta agtcgccgcg gccccgcgcg atccactgcc gcagggcacg
     6601 ccgccgcacc gcccgcgctg ccgacgccaa cgcctcgacg cgcagcggcc acgccccacc
     6661 ggcggccgaa gtttcttcac tctcggactc aggggtagcg tcttccaaca gacgcgccgc
     6721 ctccgcttcg agcgccgacg cgtcctcgcc cagaagctcc gccgtgcgcg cgagcgcctc
     6781 gaccgcgcgc gggttaaaag tctccagcaa cggcaacagc cggcggcgca cgcggacgcg
     6841 cgcgaaccgc tcgtcctcgt tcatctcgtc ggccctgaac tcgacgccgc gctcgcggca
     6901 ataccccaca gtgtcagcgc gccgcgccca cgcgaccagc ggacgcagca gccgcacgcc
     6961 ctcgcccgcc gtcgcgtcgc tggccctgtt ctcaatcgtc attctctccg acagctcgcg
     7021 ctccggcagg attcccgcaa ggcccccggc gccgctcccg cgcagtagac gcagcaggat
     7081 cgtctcggcc tgatcgtcga gcgtatgcgc cgtcagcacc gcacacgcgc cgtgagcgcg
     7141 cgccacgtcc cccaaaaact cgtaacgcgc gccgcgcgcc gcctgctcca gattatcctt
     7201 ccgcgcgcgc gcgcgcgcct ccacgtccgc ccggcccacg accgccgcta accccagcgc
     7261 cgacgcttgc gccgcgaccc acacggcgtc cgcctcaccc cgctcgcccc gcaggccatg
     7321 atcgaggtgg gccacgatca cgcacaggct cacacgtccc gcgcgcgtga gttcatcgag
     7381 cgccagcagc agcgccgccg agtcagcccc gcccgagacg gcgacgacca cgtgcgccgc
     7441 atccgagggc cacccgcgac gctcccactc gtccagcagt cgcccggcga agtcggacag
     7501 cccgcgccgc cccgtgcggc cgacgccgcg cgcgcgtttc tccgtggccg tcttcataaa
     7561 atgtcacatg gagtcaggag ccagaagtca ggagtcagaa taaatagagc gaacctgttt
     7621 ttattctgac tcctgacttc tggctcctga ctcccttttt tttgcaatct cctgccacaa
     7681 gcccgtcgcc ggtggtgttt tctgtagaga gcaccgaaat ttcgcgccat agttcacggc
     7741 ccgaagaccg cccgctcaat ccgtcagccg ataatagaac aggagaatcg aaatggagca
     7801 gcgaaaagac acgcgcgccg cccgcctccg ccgggacact tcaaccaaag cattacggct
     7861 catcgtcgcg ctcgtcctgc cggccatgct cggcctcgtc tcgaccccgc acgccgccca
     7921 cgcggccatg agcgctgatg acgccagcgc cgccgccgcc gtcgtcacca acaccctcgg
     7981 cgtgcaggag cgcttcgagg gccagtggct catcgacttc acgcagcgcg cggacggccg
     8041 cgtccagctc accctccgct accgcaacga gaaaggcact aagggcgact ccgacggctg
     8101 gaactggaac gactctttcg gcatcgacgc ctcacagctc cacggcctcg cgcgcgagac
     8161 agcgatgtcg gccgccggca cgagcgtccg cttccagatc aagcgcgacg ccggcgccat
     8221 cgactgcgag ggctggttcc g
//
LOCUS       DASVES010000233         7191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_411647, whole genome shotgun sequence.
ACCESSION   DASVES010000233 DASVES010000000
VERSION     DASVES010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411647"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VF240_09480"
     CDS             complement(<1..1059)
                     /locus_tag="VF240_09480"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7175476.1"
                     /translation="MNDYRNLASELVAAFRKQGVDASDVFVVNSSNFNTTVRVGQIEK
                     LQQSISKGLGMRIFKGGAQALTFTTDFTDKSVKSLAAQALDIVRVSSPDKFNGLAPKE
                     LLGSYDGKLQMFDDSLASVTPQRKIEIAKEMEAAGRALDKRINNSRGSSWSDTTRQVT
                     LANSDGFAGQYRTTFANMSLSMLAEENGVKQTDYWFSGNRFVSKLDTPKAVGEEAARR
                     AIRKLGGRKVKSQKVPVVVPPEVASDLVSMVFGAASGGSIFRKASFLVDKVGAEIGSP
                     LVTIIDDATIEDGLASRPFDGEGVRSSRVTVVEGGVLKTYICDSYSGRRLNVKPTGHA
                     GRGYSSGPSVNATNLYMKA"
     gene            complement(1089..2624)
                     /locus_tag="VF240_09485"
     CDS             complement(1089..2624)
                     /locus_tag="VF240_09485"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7175477.1"
                     /translation="MQRRDFMTRGSIALAAVATSDLWLGRVVAGEELKSLPILDTYFQ
                     VSREDMQKLLGAALSKGADFADLFFEYRITSALNFEEDIVKSARRGIVSGVGIRALKG
                     DQVGYAFSEDLTMPSMLEAARAAAAIANDSTARAKVVGISELSPKNLYPVVDLATTAD
                     LTKKLALIKEANEAAKAYSPKVARVQVFFNDEVKYLAYANSEGKHWQDMQPLFTFTTG
                     CIAEEGTNRQQAYETGGGRIGLEYFAKKRKPADIAREAARLAVLNLGAQEAEAGPQTI
                     VLGPGDSAVLLHEAVGHGLEADFNWKKLSNYSNRVGEKVASDLCTVIDEGLFANMRGT
                     INVDDEGNLPTATTLIENGTLRGYMADRISAKQLGVKPTGNGRREAYNHVPMARMTNT
                     YMLPGKSDPEEIIKSTKKGIYAKRFAGGQVDITKGDFTFTVLESYMIEDGKITAPLKD
                     VTLIGNGPDVMTKVTMVGNDLKFTDGGWTCGKSGQSIPVGMGIPTTKVSEITVGGTKV
                     RKA"
     gene            complement(3046..3249)
                     /locus_tag="VF240_09490"
     CDS             complement(3046..3249)
                     /locus_tag="VF240_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175478.1"
                     /translation="MAELIGNRVGAFVAACLMSVCPATAPTGLEVHARAELLDGTSWT
                     VPTLIGATLYVRDRRTITAFQLK"
     gene            complement(3338..3586)
                     /locus_tag="VF240_09495"
     CDS             complement(3338..3586)
                     /locus_tag="VF240_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175479.1"
                     /translation="MWKNLEYRVCAVQNARVTFVNNDWQGRVEPAEGNHDEALNSCPQ
                     VWDYLNEQGRYGWELAAVAPQNIGEVAMQTLYLKREAP"
     gene            complement(3599..4036)
                     /locus_tag="VF240_09500"
     CDS             complement(3599..4036)
                     /locus_tag="VF240_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175480.1"
                     /translation="MKRFACAATLLTGAALALAACSKPAEPVAFAEVCAAGTDKKTVV
                     TEGYLRPGASIMCSNTGGGPVTCGLGLFDPQGGEKKISAYVEQGTGASEVEKPASGYK
                     QGDLKLRADDSSLVGPNDRLRVTGRVTNGDNVCFLNISKIEKQ"
     gene            4226..6298
                     /locus_tag="VF240_09505"
     CDS             4226..6298
                     /locus_tag="VF240_09505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7175481.1"
                     /translation="MMRRYSSRLCALALALAACAVSAEAQSARLTVEELLKIQRVGDP
                     QLSPDGKWVAYTVAVPDVAANRSRTQIYRVAVAGGEPKRLTDGAASSTTPRWSPDGRK
                     IAYTTGGQIWKMDADGDDKEQLTKISTGASDPVWSPDGKLIAFVSDIYPDCRDDECNQ
                     KRDAQVESSKVKARTVERLLYRHWTSWKDNKRSHVFVVGSDGGTARDLTPGDFDAPPF
                     SLGGPTDYAFSPDSRELAFARNTDKAEALSTNSDLFIVPVAGGEPRRITGTNKGADQS
                     PVYSPDGRFIAYRSQATAGFESDRWRLMLYDRATGRARDLLPRFDAYVESITFTPDSQ
                     RVYFTSGERGRSPVYVVSVDGGQPAKVIDGFNDDVKVSPDNRFLVFTRSTAARPTEIY
                     TVRLDGVVPPGRGGGDQAVQLTHANDDLMRGFSLRDAEEVMWAGAGGTRVSGWLVKPA
                     NFDASRKYPLVVLIHGGPQGAWNDSWGYRWNPQVFAAHGYVVFQPNPRGSTGFGQQFT
                     NEISGDWAGRVYTDLLNGVAKVAAMPFVDKQNVAAAGASYGGYMVNWILGHNTDPRVQ
                     FKTLVSHAGVFNLTSMYGVTEELWFPEWEFKGTPWTNPAMYDRWSPHRFARNFKTPTL
                     VTHGELDYRVPVGEGLQLFTYLQRQGVESKLLVFPDEGHWILKPANSQHWYAAVIGWL
                     DSHLKRGT"
     gene            6518..7018
                     /locus_tag="VF240_09510"
     CDS             6518..7018
                     /locus_tag="VF240_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175482.1"
                     /translation="MIHHISIPAENPRRVAEVLAEIWGGRCLAFPPFPGSYIVIVDDG
                     RGSAIEVTPLGMELAPGVGDEQAQALANDNASPFTATHVALSVPAREERIKEVAAREG
                     WRAVTCDRGGAFEVVEFWIENRLLVEMLPPAMQRRYLASMTSSNYAELFGIELDTPAA
                     QELVAA"
BASE COUNT         1129 a         2376 c         2389 g         1297 t
ORIGIN      
        1 ggccttcatg tagaggttcg tcgcattgac cgaaggcccg gacgagtagc cgcgcccggc
       61 gtgccccgtc ggcttcacgt tcagacggcg gcccgagtac gagtcgcaga tgtaggtctt
      121 caagacgccg ccctcgacca ccgtcacgcg cgaggagcgc acgccctcgc cgtcgaaggg
      181 gcgcgaggcg aggccgtcct cgatagtcgc gtcgtcgatg atcgtgacga gcggcgagcc
      241 aatctcggcg ccgaccttgt ccacgaggaa cgacgccttg cggaagatgc tcccgcccga
      301 ggccgcgccg aagaccatcg agacgaggtc ggaggccacc tccggcggga cgacgaccgg
      361 caccttctgc gacttgacct tgcgcccgcc gagcttgcgg atggcgcggc gcgccgcctc
      421 ctcgcccacg gccttcggcg tgtcgagctt cgagacgaag cggttgccgg agaaccagta
      481 gtcggtctgc ttcacgccgt tttcttcggc cagcatgctc agggacatgt tggcgaaggt
      541 cgtccggtac tgccccgcga agccgtcgga gttggcgagc gtcacctgcc gtgtggtgtc
      601 cgaccagcta gagccgcgcg agttgttgat gcgcttgtct aaggcgcggc cggcggcctc
      661 catctccttg gcgatttcaa tcttgcgttg cggggtgacg gaggccagcg agtcgtcgaa
      721 catctggagc ttgccgtcgt aggagccgag gagttccttc ggcgcgaggc cgttgaactt
      781 gtcggggctg gagacgcgga cgatgtcgag cgcctgcgcg gcgaggctct tgacggactt
      841 gtcggtgaag tcggtggtga aggtgagcgc ctgcgcgccg cccttgaaga tacgcatgcc
      901 gaggcccttc gagatcgact gctggagctt ctcgatctgg ccgacgcgca ccgtggtgtt
      961 gaagttggag ctgttgacga cgaacacgtc cgaggcgtcc acgccctgct tgcggaaggc
     1021 ggcgacgagt tcgctggcga gatttctgta gtcgttcatt gcttcggtct cctttgcgtc
     1081 gcggcccttc aggccttcct gaccttcgtg ccgccgacgg tgatctcgga gaccttcgtc
     1141 gtcgggatgc ccatgccgac ggggatgctc tgccccgact tgccgcacgt ccagccgccg
     1201 tcggtgaact tgaggtcgtt gcccaccatc gtcaccttcg tcatcacgtc ggggccgttg
     1261 ccgatgaggg tcacgtcctt caggggcgcg gtgattttgc cgtcctcgat catgtagctc
     1321 tcaaggacgg tgaacgtgaa gtcgcccttc gtgatgtcca cctgaccgcc ggcgaagcgc
     1381 ttggcgtaga tgcccttctt cgttgacttg atgatctcct cggggtcgga ctttccgggc
     1441 agcatgtagg tgttcgtcat gcgcgccatc gggacgtggt tgtaggcttc gcgccgcccg
     1501 ttgcccgtgg gcttgacgcc gagctgcttg gcgctgatgc ggtcggccat gtagccgcgc
     1561 agcgtgccgt tctcaatgag tgtggtggcg gtggggaggt tgccctcgtc gtccacgttg
     1621 atggtgccgc gcatgttggc gaacagaccc tcgtcgatga cggtgcagag gtcggaggcg
     1681 actttttcgc ccacgcggtt cgagtagttc gacagcttct tccagttgaa gtcggcttcg
     1741 aggccgtggc cgaccgcttc gtgcaggagc acggccgagt cgccgggacc gaggacgatg
     1801 gtctgcgggc cggcttcagc ctcctgcgcg ccgaggttca ggacggcgag gcgcgcggcc
     1861 tcgcgggcga tgtcggcggg cttacgtttc ttcgcgaagt attcgaggcc gatgcggccg
     1921 ccgccggtct cataagcctg ctggcggttc gtgccctcct cggcgatgca cccggtggtg
     1981 aaggtgaaca ggggctgcat gtcctgccag tgcttgccct cgctgttggc gtaggcgaga
     2041 tacttgacct cgtcgttgaa gaagacctgc acgcgcgcga ccttggggct gtaggctttg
     2101 gcggcctcgt tggcctcctt gatgagcgca agtttcttcg tgaggtcggc cgtggtcgcg
     2161 aggtcaacga cagggtagag gttcttgggc gacagctcgc tgatgccaac gaccttcgcg
     2221 cgcgccgtcg agtcgttggc aatcgcggcc gccgcgcggg cggcctccag catgctcggc
     2281 atcgtcaggt cttcggagaa ggcgtagccg acctgatcgc ccttgagcgc ccggatgccg
     2341 acgccggaga cgatgccgcg gcgggccgac ttgacgatgt cctcctcgaa gttgagcgcc
     2401 gaggtgatgc ggtactcgaa gaagaggtcg gcgaagtcag cgcccttgga gagcgccgcg
     2461 ccgaggagtt tctgcatgtc ctcgcggctc acctggaagt aggtgtcgag gatggggagg
     2521 cttttcaact cttcgccggc gacgacccgg ccgagccaca ggtcgctggt ggcgacggcg
     2581 gcgagggcaa tcgagcctct cgtcatgaaa tcacgtctct gcatgcgctt tctccatttc
     2641 cggggggctt cctatcgaac ggccacgccg gcgtgtctga aaggtaatgg tttccggttc
     2701 gcgtgaaacg cgcgcggggc tgggtacgtc gcggctcagg cggagcaaat tgactcggag
     2761 cgttcgggcg tcgggcggtt gggccgcaca cacgccggct tcctgcgagc agcgccgcag
     2821 tttaactccg acgcggggac gtgaaaatgg atttgtgacg aagcgcgcgg attcggtgac
     2881 gaaacggacg ccagcgcgtg cccgccgacg cgaatcgctg accgcgccgc cgcgcttttt
     2941 ctcagcgccc tttgcgcctc ccgcgtcctt tgcgtaagac ttcattatta cgggaggaag
     3001 atttaacgcg gagggcacag aggcgcagag gacgcagagg gtctgtcact tgagctggaa
     3061 ggccgtgatg gttcggcggt cgcggacgta gagcgtggcg ccgatgagcg tcgggactgt
     3121 ccatgacgtg ccgtcgagga gttcggcgcg ggcgtggact tcgaggcccg tgggcgcggt
     3181 cgcgggacac acggacatca gacatgccgc cacaaacgcc cccacgcggt tcccgatgag
     3241 ttcagccatc acggacgcga ggctactacg acctccttcg taggtcaaca gttccgcacg
     3301 cggcggcggc gtccggggcg cttgcgacgc gggtgcgtta aggcgcttcg cgcttcaggt
     3361 agagggtctg catggccacc tcgccgatgt tctgtggagc cacggccgcc agttcccagc
     3421 cgtagcgccc ctgctcattg aggtagtccc agacttgcgg gcacgagttg agcgcctcgt
     3481 cgtgattgcc ctcggcgggc tcgactcgcc cctgccaatc gttgttgacg aaagtcacgc
     3541 gcgcgttctg caccgcgcag acgcgatatt ccagattctt ccacatactt gcggcggctc
     3601 actgcttctc gatcttcgag atgttgagga aacacacgtt gtcgccgttc gtcacccgac
     3661 ccgtgacgcg caggcggtcg ttggggccga cgagcgagct gtcgtcggcg cgcagcttga
     3721 ggtcgccctg cttgtagccg ctcgccggct tctccacctc gctcgcgccc gtgccctgct
     3781 cgacgtaggc gctgatcttc ttctcgccgc cctgcgggtc gaagaggccg aggccgcacg
     3841 tcacggggcc gccgcccgtg ttcgagcaca ttatcgacgc gcccggtcgc aggtagcctt
     3901 cggtgacgac cgtcttcttg tccgtccccg ccgcgcagac ctcggcgaag gccaccggct
     3961 ccgccggctt cgagcacgcc gcgagggcga gcgcggcgcc ggtcaacagg gtcgcggcgc
     4021 aagcaaaacg tttcatggct cgtgtctctc cttatacaag tttgagaagt cgcggcgagg
     4081 gcgcgacaga ttaccactgc gcgcgcgccg tgttcaattc gggcgcgcgt cgggcgtgct
     4141 cccggcgcgc ggcgattctg tgataaattc tcgcgcgatc cgaccagaca agttttcacg
     4201 ccgcggacgt ttgcggagga ggggaatgat gagacgttac agttcacgcc tgtgcgcgct
     4261 ggcgctcgcg ctggccgcgt gcgcggtctc ggccgaggcc caatcggcgc ggctcacggt
     4321 cgaagagctt ttgaagattc agcgcgtcgg cgacccgcaa ctgtcgcccg acggcaagtg
     4381 ggtcgcttac acggtcgccg tgccggacgt tgcggccaac cgcagccgca cgcagattta
     4441 tcgcgtcgcc gtggcgggcg gcgagccgaa gcgtctgacc gacggggccg cctcgtccac
     4501 gacgccgcgc tggtcgcccg acggtcgcaa gatcgcctac acgaccggcg gacagatttg
     4561 gaagatggac gccgacggcg acgacaagga gcagttgacg aaaatctcca cgggcgcctc
     4621 agaccccgtc tggtcgcctg acggcaaact catcgccttc gtctccgaca tctaccccga
     4681 ctgccgcgac gacgagtgca accagaagcg cgacgcgcag gtggagtcga gcaaggtcaa
     4741 ggcgcgcacc gtcgagcgcc tgctctaccg acactggacg agctggaagg acaacaagcg
     4801 ctcgcacgtc ttcgtcgtag gcagcgacgg gggcacggcg cgcgacctca cgcccggcga
     4861 cttcgacgcc ccgcccttct ccctcggcgg ccccaccgac tacgccttct cacctgactc
     4921 ccgcgagctt gcgtttgcgc gcaacaccga caaggcggaa gccctctcga cgaacagcga
     4981 cctcttcatc gttcccgtcg cgggcggcga gccgcgccgc atcacgggca cgaacaaagg
     5041 cgccgaccag tcgcccgtct actcgcccga cggccgcttc atcgcctacc gctcgcaggc
     5101 cacggccgga ttcgagagcg accgctggcg gctgatgctc tacgaccgcg ccacgggccg
     5161 cgcgcgcgac ctgctgccgc gcttcgacgc ctacgtcgag agcatcacct tcacgccgga
     5221 ttcgcagcgc gtctacttca cctcgggcga gcgcggccgc agccccgtct acgtggtctc
     5281 cgtcgatggc ggccaacccg cgaaggtcat cgacggcttc aacgacgacg taaaggtctc
     5341 gcccgacaac cgcttcctcg tcttcacgcg ctcgacggcc gcgcggccga ccgagattta
     5401 cacagtcagg ctcgacggcg tcgtgccgcc cggccgtggt ggcggcgatc aggccgtgca
     5461 gctcacacac gcgaacgacg acctgatgcg cggcttctcc cttcgcgacg ccgaggaagt
     5521 catgtgggcg ggcgcgggcg gcacgcgtgt ctcgggctgg ctcgtcaaac cggcgaactt
     5581 cgacgcgtcg agaaagtatc cgctcgtcgt cctcatccac ggcgggccgc agggcgcgtg
     5641 gaacgacagt tggggctacc gctggaaccc gcaggtcttc gccgcgcatg gctacgtcgt
     5701 cttccagccg aacccgcgcg gctccaccgg cttcgggcaa cagttcacca acgagatttc
     5761 cggcgactgg gcgggccgcg tctacaccga cctgttgaac ggcgtggcga aggtcgccgc
     5821 gatgcccttc gtcgataagc agaacgtcgc cgcggccggc gccagctacg gcggctacat
     5881 ggtcaactgg atactcggcc acaacaccga cccgcgcgtc cagttcaaaa cactcgtctc
     5941 acacgccggc gttttcaatc tgacgagcat gtacggcgtg accgaggagc tgtggttccc
     6001 cgagtgggag ttcaagggga cgccgtggac gaaccccgcg atgtacgacc gctggtcgcc
     6061 gcatcgcttc gccagaaact tcaagacgcc gacgctcgtc acgcacggcg agcttgatta
     6121 ccgcgtaccc gtcggcgagg gcctgcaact gttcacctat ctccagcgac agggcgtcga
     6181 gtccaaactc ctcgtcttcc ccgacgaggg ccactggatt ctcaagcccg ccaactcgca
     6241 gcactggtac gcggccgtca tcggctggct cgactctcac ctcaaacgcg gcacctgagt
     6301 cgctcgcgcc acgagcgacg ccgtcaagcc tttcgcacac acccgcggcc ccgtcgcctc
     6361 tcacgaagcg cggggccgct gccttttctt ctctcccgaa attatttcta cggggcatga
     6421 tcctccgcgc tgattttcca cgcgttacac ggcggggata caagaacatg gcgcgcggcc
     6481 ccggcgtgcg gaacttccga cagagaggat aggggcaatg attcatcaca tttcgatacc
     6541 ggcggaaaat cctcggcgcg tcgccgaagt gctggcggaa atctggggcg ggcggtgtct
     6601 ggcgttccct cccttccccg gcagctacat cgtcatcgtg gacgacggac gcggcagcgc
     6661 catcgaggtc acgccgctcg ggatggagct ggcgccgggc gtgggcgacg aacaggcgca
     6721 ggcgctggcc aacgacaacg cctcgccgtt cacggccacg cacgtcgctc tgtccgtgcc
     6781 ggctcgggag gagcgtatca aggaggtcgc cgcgcgcgag gggtggcgcg ccgtgacgtg
     6841 cgaccgcggc ggggccttcg aggtggtcga gttctggatc gagaaccgcc tgctcgtcga
     6901 gatgctcccg cccgcgatgc agcgccgtta cctcgcctcc atgacctcat ccaactacgc
     6961 cgaactcttc ggcattgaac tcgacacgcc cgccgcgcag gaacttgtcg ccgcctgaag
     7021 acggaacagt accgcgagcg gtagcgagcg ggtctggccg ggcgatgcgg gtctctcatc
     7081 acaggcgtac ccgctcgcta ccgctcgcgg tactgtcacc cgttacgggc gcggctcgtc
     7141 gccgggcgcg ctacgccggg ctatctcatc atcccttcgg ggcttctcaa a
//
LOCUS       DASVES010000234         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_209581, whole genome shotgun sequence.
ACCESSION   DASVES010000234 DASVES010000000
VERSION     DASVES010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209581"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /gene="mutS"
                     /locus_tag="VF240_09515"
     CDS             <1..1368
                     /gene="mutS"
                     /locus_tag="VF240_09515"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7175483.1"
                     /translation="IIRAGFSDELDSLRKLLREGKSFLAEMEQRERARTGIKSLRVGY
                     NKVFGYFIEVTRPNLRLVPADYTRKQTLTQSERFITLELKEYESLVLNSQERIAELES
                     SLLRRVLSEIGKRRDDVLSAAAALAHLDAVASFAQNAARWNYVRPEVVEEPVLRIEGG
                     RHPVLERTLVDEEFVANEVRLGGEGAPDIALITGPNMSGKSTYLRQTALIVLLAQTGC
                     FVPAARAKVGLADRIYTRIGLYDRLGSGESTFMTEMVETAHILHHATPRSLILLDELG
                     RGTSTYDGLAVARAVLEHLHNHPKLRARTLFATHYHELTEAADLLARVGNLHVEIAEL
                     EGELTFLYKVVPGSAERSFGVYAAKLAGLPKPVVRRAEELLRQYEAGEVDTSAVADLD
                     AERLLGERLNAAHTAPTESPLVRALLEADPESLSPVEALMKLIELRRMAEGEKSKDVK
                     VAKTA"
     gene            complement(1365..2357)
                     /locus_tag="VF240_09520"
     CDS             complement(1365..2357)
                     /locus_tag="VF240_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7175484.1"
                     /translation="MPEFKKRLPTTQAGDDAGVSGGAGAGPHLALVAVQLFFGTWPIV
                     GKVGLRSLPSTGLVALRVGGAALALYVLRSIVGGVPIRRRRDFAHIFLYSLLGVVLNQ
                     FLFVKGLSLSTAVNATVLGTAIPVFALLVSVALGSERLTPRKAVGTLIAAAGVVWLVD
                     PFRADFAGDKTLGNILLVANTLAYGAYIAISQDMFRRYGALTVITWVFVFGSILAVPL
                     GGFYLAQVPLAELDLSVWLAIAYIILVPTVGAYYLNAWALARVAPSTVAAYIYLQPLI
                     AFAIAPLVLGAEEFGGVRTLLAALLIFSGVALVTLAPASKASETLSEHPDAIGH"
     gene            complement(2403..2600)
                     /locus_tag="VF240_09525"
     CDS             complement(2403..2600)
                     /locus_tag="VF240_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175485.1"
                     /translation="MSEKDSVLPIPQQEGQQSSENESGDKAGAAAGDATAYRKGEEDA
                     TMLREEERTGEGTGAKAGEYS"
     gene            complement(2627..>3996)
                     /locus_tag="VF240_09530"
     CDS             complement(2627..>3996)
                     /locus_tag="VF240_09530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7175486.1"
                     /translation="IAEIDTDKATMEAQALTTGVLRKILVPAGETVPLGAIIAVIAEA
                     DEDISDILSKAGSAQTTTARKEDTQEPVKTGGDGSETESSGVVPGGERTPATVEGQPI
                     AGDVGGAPAAKDSDDSDDTLEKSQPPAPKGGQAAGNGQGEQSSGRLLVSPIAARMAAE
                     AGINLKTLSGSGPGGRIIKRDIEAAITSGPQSQQQQTSPEAKPAEQPAPAVKTQATAR
                     PATQSAVEGASPYRDEPVSTMRATIARRLVSSIGPIPHFFLTTEIEMDRAAEMRRQIN
                     ELDTELKISVNDLIIKVAAVSLIQHPAVNASFQDKVVRYYERADIGVAVAVEEGLITP
                     VVRGAESKSVGQISREVRELAERARARRLKPEEYTGATFSVSNLGMFGIDEFTAVINP
                     PEAAILAVGAMQPKPVVRDGQVVVRQLMRVTMSCDHRVVDGATGAKFLQTFKKLLENP
                     LLMIV"
BASE COUNT          700 a         1278 c         1303 g          715 t
ORIGIN      
        1 atcatccgcg ccggcttctc cgatgaactg gactctctcc gcaagctctt gcgcgagggc
       61 aagagctttc tcgccgagat ggagcaacgc gagagggcgc gcaccgggat taagagtctg
      121 cgcgtgggct acaacaaagt cttcggctac ttcatcgagg tcacgcggcc gaacctccgc
      181 ctcgtgcccg ccgattacac gcgcaagcag acgctcacgc aatcggagcg gttcatcacg
      241 ctggagctga aagagtatga gtcgctggtg ctcaactcgc aggagcgcat cgcggagctg
      301 gagtcgagcc tgctccggcg cgtcctctcc gagatcggca agagacgcga cgatgtactc
      361 tccgccgccg ccgcgctcgc gcacctcgat gccgtcgcct ccttcgcgca gaacgcggcg
      421 cgctggaact acgtgcggcc cgaagtcgtc gaggagcctg tcctgcgcat cgagggcggg
      481 cgccaccccg tgctcgaaag gactctcgtt gatgaagagt tcgtggccaa cgaggtgcga
      541 ctcgggggcg agggcgcgcc agacatcgca ctcatcacgg ggccgaacat gtccggcaag
      601 tcaacctatc tgcgccagac cgcgctcatc gtcctgctcg cacagacagg ctgcttcgtc
      661 cccgccgccc gcgcgaaagt tggcctcgcc gaccgcatct acacgcgtat cggcctctac
      721 gaccggctcg gcagcggcga atcaacgttt atgaccgaga tggtggagac cgcacacatc
      781 ctccaccacg cgaccccacg ctccctcatc cttctcgacg aactcgggcg cgggacatcc
      841 acctacgacg gcctcgccgt cgcccgcgcc gtgctcgaac acctgcacaa tcacccgaaa
      901 ctgcgcgccc gcacgctgtt cgcaacgcac tatcacgaac taaccgaggc ggccgacctc
      961 ctcgcccgcg tcggcaattt gcacgtcgag atcgcggagc ttgagggcga gttaactttt
     1021 ctctacaagg tcgtgcccgg ttctgctgaa aggagtttcg gcgtctacgc ggccaaactc
     1081 gccggactgc cgaagcctgt cgtgcgtcgc gcggaggagc ttctgcgcca atacgaggcc
     1141 ggggaagtgg atacttccgc ggtggccgat ctggacgccg agaggcttct gggcgagcga
     1201 ctcaacgccg cacacaccgc cccgacggag tcgccgctcg tcagagcact actcgaagcc
     1261 gacccggaat cgctctcgcc cgtcgaggcg ctgatgaagc tcatcgagtt gcgccggatg
     1321 gccgagggtg agaaatcgaa agacgtgaaa gtcgcgaaga cagcttaatg cccgatagcg
     1381 tcggggtgtt cagacaaagt ctccgacgct ttcgaggcgg gggcgagtgt gacgagcgcg
     1441 actcccgaaa aaattaacaa agccgcgagc agggttcgca cgcccccaaa ctcttcggcg
     1501 ccgaggacga gtggggcgat ggcgaacgcg ataagcggtt gcaggtatat gtatgccgcc
     1561 accgtgctcg gggcaacgcg agcgagcgcc cacgcattga ggtagtaagc gccaaccgtt
     1621 gggacaagga tgatgtatgc gatggcgagc cagacggaaa ggtctaactc cgcgagcggg
     1681 acttgcgcga ggtagaagcc gccgaggggc acggcgagaa tgctcccgaa gacgaagacc
     1741 caggtgatga ccgtgagcgc gccgtaacgc cgaaacatat cttgcgagat ggcgatgtaa
     1801 gctccatagg caagcgtgtt cgcgacgagc aggatgttgc cgagggtctt gtcgcccgcg
     1861 aagtccgcgc ggaagggatc gacgagccag acgacgccag cggcggcgat gagagtgccg
     1921 accgccttgc gcggcgtgag ccgttcgctg ccgagggcga cgctgacgag gagcgcgaag
     1981 acggggatgg ccgtgccgag aacggtcgcg ttgaccgcgg tcgagagtga gagacccttg
     2041 acgaagagga actgattgag gacgacgccg agaaggctat agaggaagat gtgtgcgaag
     2101 tcgcgccggc gtcggatggg gacgccgccg acgatactgc ggaggacgta gagggcgagg
     2161 gcagccccgc ctacgcgcaa cgccacgagt cccgtgctcg gcagcgagcg caacccgacc
     2221 ttgccgacaa tcggccatgt tccgaagaag agttgcaccg ctacgagcgc cagatgaggc
     2281 cccgcgccgg ccccgccgct aacgcccgca tcgtccccgg cctgagtagt cgggaggcgc
     2341 tttttaaact ccggcatcga tgcacggtgc ggccataaaa gctggcatga gtttaacggg
     2401 cgtcagctat actcaccggc cttcgccccc gtgccttcgc ccgtcctctc ttcctctctg
     2461 agcatggtcg cgtcttcctc gcccttccgg taggcggtcg cgtcgccggc ggcggcgccc
     2521 gccttgtcgc cagattcgtt ctctgatgat tgctggccct cctgctgcgg aatgggcagg
     2581 acggaatctt tttcgctcat gtcggctccc cttcggttct tcaatttcaa acgatcatca
     2641 ataacgggtt ctcaagaagt tttttgaaag tctggagaaa cttcgcgccc gtcgcgccgt
     2701 caacgacccg gtgatcgcac gacatcgtga cgcgcatcaa ctgtcttact acaacctgtc
     2761 cgtcacgcac gaccggcttg ggctgcatcg cgccgacggc gaggatggcc gcctcgggtg
     2821 ggttgatgac ggccgtgaac tcgtcaatgc cgaacatgcc gagattgctg acggagaatg
     2881 ttgctcccgt atactcttcg ggcttgagcc ggcgcgcgcg cgcccgttcg gcgagttcgc
     2941 gcacctcgcg gctaatctgt ccaaccgact tgctttcggc gccgcgcacc accggcgtga
     3001 tgagtccttc ctcgacggcc acggcgacgc cgatgtcggc gcgctcgtaa tagcgcacga
     3061 ccttgtcctg gaaggaggcg ttgaccgccg ggtgctggat gagcgaaacg gccgcgacct
     3121 tgatgatgag atcgttaacg ctaatcttta gctcagtgtc gagttcgttg atttgccgcc
     3181 gcatctcggc cgcgcgatcc atttcgatct cggtcgtgag gaagaaatgc gggatggggc
     3241 cgattgagga gaccagtcgg cgcgcgattg tggcgcgcat cgtcgagacc ggctcgtcgc
     3301 ggtaggggga cgcgccctca accgccgact gcgtggcggg tcgggccgtc gcttgagttt
     3361 tcacggcggg cgcgggctgc tcggcgggtt ttgcttccgg cgaagtttgt tgctgttgcg
     3421 actgtgggcc ggaagtgatg gccgcttcga tgtcgcgctt gatgatgcgg ccgccggggc
     3481 cgctgccgga gagcgtcttt aggttgatgc ccgcctccgc cgccatccgc gcggcgatgg
     3541 gcgagacgag caggcgtccg ctcgactgct cgccctgtcc gttaccggca gcctgcccgc
     3601 ctttgggcgc aggtggctgt gacttttcca gcgtgtcgtc tgagtcgtcc gaatctttag
     3661 cggcgggcgc accaccgaca tcacccgcga ttggctgccc ctcgacggtc gccggcgtgc
     3721 gctcgccgcc gggcacgacg ccggacgact ccgtctctga gccgtcgccg cccgtcttca
     3781 ccggctcctg tgtgtcttcc ttccgcgcgg tcgtcgtctg tgccgagccg gctttagaga
     3841 ggatgtcgga gatgtcttcg tcggcctcgg cgatgaccgc gatgatcgcg ccgaggggga
     3901 cggtctcgcc ggcgggcacg aggatttttc gcagcacgcc ggtcgtcagc gcctgcgcct
     3961 ccatcgtggc cttgtccgtg tcgatctccg cgattg
//
LOCUS       DASVES010000235        17303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_758324, whole genome shotgun sequence.
ACCESSION   DASVES010000235 DASVES010000000
VERSION     DASVES010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17303
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758324"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..313
                     /gene="infA"
                     /locus_tag="VF240_09535"
     CDS             92..313
                     /gene="infA"
                     /locus_tag="VF240_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX7175487.1"
                     /translation="MSKEDLVPAMGVIVDKQPNAFFKVQIDNSDHVVLAQISGKMRKN
                     RIRILVGDRVSVEMSPYDLTRGRITYRYK"
     gene            complement(515..2233)
                     /locus_tag="VF240_09540"
     CDS             complement(515..2233)
                     /locus_tag="VF240_09540"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7175488.1"
                     /translation="MLAERFLLTSRLHSSPTRRATLALALFVAVATSPSPHDFSFKAA
                     WPAVVAVAQDDPLRALTDEYAQLESQGLKENFHRQLSYTMLQEYLPSGTRMTSGYRSP
                     EKQLGLILRMARAQGIPAPAQGSVEDESSWRPALMGLRSKGYIVAAPTTTPHATDEAV
                     FDLSGADLNAIQQGLRRAESAGMVKFRRIIFEAQNNAIHVEVESISPKALNALGRRRP
                     ASGGGTSSGTGGGTTGGGGGTAPASEADQQRSMVQQLQNLHDSEPDPAKKIDYDRSQK
                     NLLNPADDAARIAVLDEEIARHQEEAQQLTGDTRRKQAVEGVSEALRDERYEDAEQAA
                     ELLVESYPDIPEAKDMLAQIRTRRLIDAATDAIYASDEPACDECEKASELMTEALELS
                     PDYEGAQFIKEDIDACLARCKTGRLPFIILSLLLLAGAGAGLYYLARSGNLLPRLAGA
                     LKGSGKSPVSGGGGAARGWVLEGIDGACRGQVFPLEKAELVIGSKGPPEGVADIVVLD
                     AQRKISRRHCTVMQNGKQFYLMDESTNGTSLNGQPAARGTYVEFRAGDHISLADVAVL
                     RLRPKG"
     gene            2316..3116
                     /locus_tag="VF240_09545"
     CDS             2316..3116
                     /locus_tag="VF240_09545"
                     /inference="COORDINATES: protein motif:HMM:NF022450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase Amj family protein"
                     /protein_id="HEX7175489.1"
                     /translation="MSTQVALVFVLTFIIHLISTLAFALRIAGVRTGRIAVSLALFNV
                     LVLVSRTSNTFQGPLLAKHVERNIIGGRMEGAEWDFRWLLLAATLATLVGGLLVPTFQ
                     RLFTRAIGTFSVHRSVPRLLLRAVTPVGLRALREATSIPAKENLTRMSLRRAPLRIVV
                     YNVVSTSLITVGVLSSLYAGYLNPELRVTANNMSPVINGLSTILLFIYIDPHLSIMSD
                     DVAGGRATDAEYRRFVTLLVVSRLAGTLLAQLLLVPAARVIVFVARSL"
     gene            3380..4447
                     /locus_tag="VF240_09550"
     CDS             3380..4447
                     /locus_tag="VF240_09550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7175490.1"
                     /translation="MQTDVKTEPSSGGGTQQQRAPQLSVVMPVRNGLPFLDDSVRSIL
                     GQTFADFELIVLDDASTDATLAALREWERRDSRVRVYESGRKLGLSGSSNFVVEQARA
                     PIVARMDADDVAHPERLARQFEVMRGRPEVVLVGALSEGIDADGRSVRPRDRWRLLRS
                     SPFPPFPHGSVMFRRAAFDAVGGYREECVNWEDQDFFLRLVRRGRVVVLPETLYYYRY
                     HLTNSTGGLPVEHLAHLYAIRSLCLEEFRTGRDYEGVLEQAARSTPNGSGPKAAADAL
                     YQTGAMRLWAGRAPAILGDTLRHGGLRLGGRSLMALVLGVWGGVSPVSLRASLRSFIR
                     ARDLLAGWRMKNGSPREWRLK"
     gene            4432..5451
                     /locus_tag="VF240_09555"
     CDS             4432..5451
                     /locus_tag="VF240_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7175491.1"
                     /translation="MALKVTVVGLGGRGRQWAREVSAAPGFELAAGVEPDGEALRRAR
                     SESGLPSEKCFADLDEALERTACDAVIVATPAERHAAACEATLARGLAVMVEKPFTMR
                     LAEAVRIVELAERKRAPLIVAQNYRYMRAFRTARRLVRSGALGRVGSVVCQYYRVPHE
                     MAASLARLPHRVLWGMGVHHLDALRHVLGREATGVCAESYTTAWGEPPEGASMRATLS
                     FEGGARGFYSATYESSGHEYFERGQEFYLRFTGETATLHVFHRWLVLCERGRLPRPVR
                     RGPRRVSEERILLDQLEGALLRGEEPDASGRDNLQTMAIVEACLRSAAERRWINPQEL
                     LDEQG"
     gene            5438..6958
                     /locus_tag="VF240_09560"
     CDS             5438..6958
                     /locus_tag="VF240_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7175492.1"
                     /translation="MNRGRQTRVLAVGIDAAEPTLVRELIERGEMPVLKSLLDEGAWL
                     RVESGAHVGSGAVWPTFITGASPAEHGIYGEWAWQPETMSLARFNARGLKPFWEPLAT
                     RDGLTVGVLDVPFGPLVGLTRGFEISEWGAHDTLEGRTEFAPASLSAILNGKTGAHPF
                     TQKHIDPAGPIDYPEMRNLSAACIEGARLRGALAEQLIQQTRPDFALITFTEVHHGAH
                     HAWHTVAPEHELYEADSFRSLRAVEPTLIDLYREVDRQTGRLVAAAGDGASVFVFSLH
                     GMRPSRGLPTLLAPLMCELGWARRADLSTQSWTGRALSLVAAVKRRMPKAIKKLYYRT
                     MPKAATYRLAQPTMMPAYDWSRTRAFPVHTDQHGWIRVNLAGREARGLVPVAEYEETC
                     RRLEDAMRALTTEDGRPLVRDVIRTAPRTEDALALRLPDLILHWHDAAFDSPVRVQGT
                     SIEAYPTGAKFTGQHGLEGFLILSGQASTPAGDHIDGRDLHRLILNALGDRRASKT"
     gene            complement(7038..8249)
                     /locus_tag="VF240_09565"
     CDS             complement(7038..8249)
                     /locus_tag="VF240_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7175493.1"
                     /translation="MDHGVEILLSLFVVFVAAQIGGEIAQRIKLPAVVGEIAAGCLIG
                     PYALGWIKLNEPLEVLAEIGAVLLLFSVGLETRVGDLRKVGRVASFVGVLGVVLPFLL
                     GGLWAWLSGYETPKAMFIAAAFVATSAGITARVLQDLGVLGRTESRVILGAAVIDDVL
                     AMLLLGVVTAVQGGGGVNVVGLLLILAQAVGFVALVALVGTRVMGRSSNLLEAPINPL
                     SPLTLSLALCLGLAAAAAWIGLAAIIGAFLAGMVMAESSQRHTLEKQFQPIMAFLVPF
                     FFVVTGAKVDVTQLGSFSVLGMVAVVTLLAVVSKLVGCGLGALSLGKKSALIVGVGMV
                     PRGEVGIIVASLGQQAGVFSETIYAVIIAMSILTSIIAPPVLKVLLAGAPPEPGDDVS
                     YLISVEETGHG"
     gene            complement(8440..8510)
                     /locus_tag="VF240_09570"
     tRNA            complement(8440..8510)
                     /locus_tag="VF240_09570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8476..8478),aa:Gln,seq:ctg)
     gene            8722..9081
                     /locus_tag="VF240_09575"
     CDS             8722..9081
                     /locus_tag="VF240_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7175494.1"
                     /translation="MGDDASLKEEVERGLEGEPRGRADARDEPARLTAELAARVRHEI
                     NNPLTGLIGQAQLLLRDDTLSETARRRVEIIEQLAKRIRDAVAELRAVRNPPGGAAGA
                     GARGPGETPRVEDPPRH"
     gene            complement(9099..9875)
                     /locus_tag="VF240_09580"
     CDS             complement(9099..9875)
                     /locus_tag="VF240_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175495.1"
                     /translation="MAAFGQDAAKIVQPSPASATADAAPARIDNRDVLKMLEARLAPE
                     VVVAKIKTSAADFDTSPEALKALRDAGAPDSVILAVVEAPRVPAVAAPAGESKKVVAV
                     KVPAGTRVEVESAYRVNSQEVRVGDLITFRVVNPVKVGGVTVVEPGAVATARVVKASR
                     GGHWGRAGRLAWEMYEVTAADGSRLQIQFAGRQVGDSKGAKVATQIALTAALLPLVAP
                     VALLHGFKRGENAYLPEGKRFDVSVRAEATVSGAASGPED"
     gene            complement(10083..10898)
                     /locus_tag="VF240_09585"
     CDS             complement(10083..10898)
                     /locus_tag="VF240_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175496.1"
                     /translation="MSLALTTALVSPAAAIMAAAQQEQQQTSQAQQTQPPSPKLINKD
                     VAEMLKAGLSAEIVVAKIRSSATAFDTSAAALQEMKAAGVPDAVILAMVEAPAGDPAA
                     VTMTTAPAAAPVEIKVPDGTEVEIELINVVSGEEVKVGSFVDFKVLHPVVLNGVTIFE
                     RDASARARITTAKKAGRWGRAGKLEWAMQDVQASDGNRVPMRFTDRALGDSKGGTVAV
                     AAVATTVLLGPIGLLWGLKKGKPAIIPAGNRYRVFVHGDTAIKGKPASAVAGS"
     gene            complement(11087..13048)
                     /locus_tag="VF240_09590"
     CDS             complement(11087..13048)
                     /locus_tag="VF240_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7175497.1"
                     /translation="MFSDNFRRGETVKSRFGGVKTSKLISNLSDVAWKTFQSVNRRLP
                     EGAAIHPKWAAEPLLKSYERTSPPLGFPRETDSLCPSCVKEVRNAVISGDVSLETLMH
                     THPGEIKAQIIEEDGRVYMTKTCPSHGEFKDLMASDSRFLARIESLFFGRDFRAAEDA
                     HIHKHGTSNIKFGRGAVLTVDLTNRCNMMCNPCFMDANQVGYVHEPTLDDTKAILDRA
                     VSFKPRRQIIILFSGGEPTISPHFLEAVSYARKIGFYRILAATNGIRFAESVEYCQQA
                     KDAGMHGVYLQFDGVSEEKNKHRGVGNLFDVKLRAIENLAAVGIKVTLVTTIVNTINN
                     DGIGQIVEFAAKNIDKVQTIAFQPVSFTGRDEDVPDNIRLQQRYTLAGMAGDLESQLG
                     GRVEALRDWFPLSSYSAFTSVMDMLQGADAPWGWSSCNCHPNCGVFTLMVVNRQTGEF
                     RSLFEFFDYEQFMKDVGVITDTARGRKLSYAQLGMAIMRNFKAERAPEGFPVSQIVNL
                     FKPSSTSSNSDRNDRMKSQGADDPSDVWRVLCVEGMWFQDLFNYDFRRTEMCVIPYGT
                     QEGEISFCAYNTGVGWRQIIENMHKTANLGEWYQEHGRHAVYAKGKHVPGIQKSRGLS
                     LPIIQAIIEDDAPAATTPYLVEEEEAAVS"
     gene            13362..14177
                     /gene="lpxA"
                     /locus_tag="VF240_09595"
     CDS             13362..14177
                     /gene="lpxA"
                     /locus_tag="VF240_09595"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX7175498.1"
                     /translation="MQAHPTAIIHPRAQLAAGVRVGPYAVVEEDVTVGAGCDIGAHAV
                     VKRFTTLGERNRVYEHAVLGGEPQDVKFGGERSHLHIGDDNLIREYATLHRASGEGAA
                     TRVGSRNFLMVGTHIAHNCEVGDDNIFANGAALAGHVRVEDHVFLASNVGAHQFTRFG
                     RYAMVGGKSKIIQDVLPFFTTDGNPPRVRGLNTVGLRRAGFSAESRRELKRAYQLLFR
                     ARAPLEEVLREMERTDDEHVRHLAAFIRGSRRGFSREQRRGRETAEETDTAEE"
     gene            14174..15229
                     /gene="lpxD"
                     /locus_tag="VF240_09600"
     CDS             14174..15229
                     /gene="lpxD"
                     /locus_tag="VF240_09600"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7175499.1"
                     /translation="MMLKGKTVAELAALVGGRAVGDGQVVVERVASLEGAGQGSVAFV
                     ADAKMMEAARASDASCLIVPEGAGPEGRCRIEAANPKLAFALVAEVLHPPKRNEPGVH
                     PTAAVEPDARLGEGVFVGAGARVGAGAEVGAGTQIQEGCVLGAGVRVGRDCVLHPHVV
                     LYEGATLGDRVVLHAGAVVGADGFGYVRDVAGVYHKFPQVGTVVIESDVEIGANSCVD
                     RGALGETRVGRGTKIDNLVQVGHNVSVGERVVIAAQTGISGSTVIESGAVIGGQVGMG
                     DHARVESGAVIGSQAGVLPGKIVRAGVWWGTPVQPLEEYKRLHALWGRLPRMREEIKE
                     LRAQLKELQARMGEKAD"
     gene            complement(15335..15778)
                     /locus_tag="VF240_09605"
     CDS             complement(15335..15778)
                     /locus_tag="VF240_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX7175500.1"
                     /translation="MTETLTPRAPVLLYDGVCGFCNGAVQTILRHDRRGSLRFAALQS
                     DYGRAALARHPELAGVDSVVFLEASPETGRERVSVRSEAGLRVASYMGGAWKIFLLGR
                     LLPASVRDYLYDLFARHRYKMFGKYDTCLLPPPEVRARFIDASQE"
     gene            complement(15775..16971)
                     /gene="lpxB"
                     /locus_tag="VF240_09610"
     CDS             complement(15775..16971)
                     /gene="lpxB"
                     /locus_tag="VF240_09610"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX7175501.1"
                     /translation="MTDGPAAERLRLMIVAGEDSGDAHAAALVGALRERAPGVSFEFF
                     GATGRRMREAGVESVVREDDLAITGLLEIGRALPRFWHAYRALRRAAVERRPDAVVLV
                     DWPDFNLRLARALSRRGLRVVYYISPQLWAWRSYRVRQVRRDVDLLLTILPFEKDWYA
                     RRGFDAVEFVGHPLAGEVRPGAGRAEFCRRHGLEASRPIVALLPGSRRKNFERIFPVM
                     LDATARLARTRPGTQFVIALAARRTRAEAEALIANVPGGADVAGRLLFVEHETREALG
                     AADAAAVCSGTATLEAALVGVPTVVVYKESFVNWNTLGRLISVEHYGLVNLIAGERVA
                     PELMQHDFTGDRLARELSALLDDERNSEVRARLRAAVARLGEGGASLRAADAVLCAVR
                     AWKEEG"
     gene            complement(17107..>17303)
                     /locus_tag="VF240_09615"
     CDS             complement(17107..>17303)
                     /locus_tag="VF240_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175502.1"
                     /translation="MEAKEWPRCQKCSQGTLIPLSDYGREGAPITYKAWVCTNPDCGF
                     NLRIDNGEISFGRAIGQSYK"
BASE COUNT         2980 a         5686 c         5747 g         2890 t
ORIGIN      
        1 aagagactcg acgccccttg acccggcccg taactgtgga ggttcaacgg agacttgata
       61 ctatctacac aaaccgcaca ggaggattcg attgtctaaa gaagatttag ttccagcgat
      121 gggcgtgatt gtcgataagc agcccaacgc gtttttcaaa gttcagatcg acaacagcga
      181 tcacgtggtg ctggcccaga tttcgggcaa gatgcgtaag aaccgcatcc gcattctggt
      241 gggcgaccgc gtcagcgtcg agatgtcccc gtatgacctg acacgcgggc gcatcacata
      301 tcggtacaaa taagataaga gacggtggac cggaatcccg atgcgccggg gcgtgaggcc
      361 gccgggcggg cttcgtatca attaatatcc gatttgttca gaggcgggcc gcgcccgccg
      421 tctcggccga caaggcttcg ggacggcggg cgcggtttgc ttttgaacct tgaagtggcg
      481 ggcgcggccg aaagccgcac cgggtcatcc cccgtcatcc ctttggccgc agacgcagca
      541 cggccacgtc ggcgagcgag atatggtcgc cggcgcggaa ctccacgtat gtcccccgcg
      601 cggccggctg cccgttgaga ctcgtgccgt tggtgctttc gtccatcagg tagaactgtt
      661 tgccgttctg catgacggtg cagtgacgcc gcgagatttt ccgctgggcg tcgaggacga
      721 cgatgtcggc gactccttcg ggcggcccct tcgagccgat gaccagctcg gccttctcca
      781 acgggaagac ctgacccctg cacgcgccgt cgattccttc gagaacccag ccgcgcgccg
      841 cgcctccgcc gccggaaacc ggactcttgc ccgaaccctt caaggccccg gcgagacgtg
      901 ggagcaggtt gccggagcgc gcgagataat agaggcccgc gcccgcgccg gcgaggagca
      961 acagggagag gatgatgaac ggcagacgcc cggtcttaca gcgcgcgagg caggcgtcga
     1021 tgtcctcttt gatgaactgc gcgccctcat agtctggcga caactcaagc gcctccgtca
     1081 tcaactcact ggccttctca cactcgtcgc acgccggctc gtccgacgcg tagatggcgt
     1141 ccgtcgccgc gtcgatcagc cggcgcgtcc tgatctgggc gagcatgtcc ttcgcctcgg
     1201 ggatgtcggg gtagctttcc accagcaact cggccgcctg ctcggcgtcc tcgtacctct
     1261 cgtcgcgcag cgcctcgctc accccctcga cggcctgctt cctccgcgtg tcccccgtga
     1321 gttgctgcgc ctcctcctgg tgccgggcga tctcttcgtc gagcacggcg atgcgcgcgg
     1381 cgtcgtcggc ggggttgagc aggttcttct gcgagcggtc gtagtcgatt ttcttggcgg
     1441 ggtctggctc gctgtcgtgg aggttctgta gctgctgcac catgctgcgc tgctggtcgg
     1501 cctccgacgc cggggccgtg ccgccgccgc cgccggtcgt cccgccaccc gtgccggacg
     1561 acgtgccgcc gccgctcgcg ggccgtcgcc ggccgagcgc gttcagcgcc ttggggctga
     1621 tgctctcgac ctcgacgtgt atggcgttgt tctgcgcctc gaagatgatg cggcgaaact
     1681 tcaccatccc ggcgctctcg gcgcggcgca ggccctgctg gatggcgttg aggtctgcgc
     1741 cggagaggtc gaagacggcc tcgtccgtgg cgtgcggggt ggtggttggg gcggcgacga
     1801 tatagccttt ggagcgaagt cccatcaggg cggggcgcca gctcgattcg tcctcgacgc
     1861 tgccctgcgc gggggccggg atgccctgcg cgcgggccat gcggaggatg aggccgagct
     1921 gcttctccgg gctgcgatag ccggaggtca tacgcgtccc cgatggcaaa tactcctgaa
     1981 gcatcgtgta ggagagctgc cggtggaagt tctctttgag tccctgactt tcgagctgcg
     2041 cgtactcgtc agtcaacgcg cgcaacgggt cgtcctgagc gacggcgacc acggccggcc
     2101 acgccgcttt gaaactaaag tcatgcggcg acggcgacgt ggcgacggcg acgaagagtg
     2161 cgagcgcgag cgtcgcccgc ctcgttgggc tgctgtggag tcgagaagtt aagaggaacc
     2221 tttccgcgag cacaagaaat gtgcgtgtgt gaatccggcg ggttaatccc cgtgcatcaa
     2281 tgctagaatc ctcgcgtcca ccccatcgtc ttaacatgag tacacaagtc gcgctggttt
     2341 tcgtcctcac cttcatcatc cacctgatca gcaccctggc cttcgcgctc aggatcgcgg
     2401 gcgtcaggac gggccgcatc gccgtctcgc tggccctctt caacgtgttg gtgctcgtct
     2461 cgcgcacgtc gaacaccttc caggggccgc tgctcgccaa gcacgtcgag cgcaacatca
     2521 tcggcggacg gatggagggc gccgagtggg acttccgctg gctgctcttg gcggcgacgc
     2581 tggcgacgct cgtcggcggg ctgctcgtcc ccaccttcca acgcctgttc acgagggcca
     2641 tcggcacctt cagcgtccac cgctccgtcc cgcggctgtt gctgcgcgcc gtcactcccg
     2701 tggggctgcg agcccttcgc gaggcgacga gtataccagc aaaagagaac ctcacgcgga
     2761 tgagccttcg gcgcgcgccg ctccgcatcg tcgtctacaa cgtggtctcc acttcgctca
     2821 tcacggtcgg cgtcctctcc tcgctctacg ccggctacct caacccggag ctgagggtca
     2881 cggccaacaa catgtcgccg gtcatcaacg gcctctcgac gatcctcctg ttcatctaca
     2941 tcgacccgca cctctcgatc atgtcggacg acgtggccgg cggccgggcc acggacgccg
     3001 agtacaggag gttcgtcacc ctcttagtcg tcagccgtct cgcggggacg ctcctcgcgc
     3061 aactgctcct cgtcccggcc gcgagggtca tcgttttcgt ggcgcggagc ctgtgacgag
     3121 aggccgcgtc tccgaggagg ttgcgcgaca atcatcagac atacaaactt gccggggctg
     3181 gagtgatgtt cacttgaatg aaagcgaaac agattcaccg ccgaggcgca ggacaaaagg
     3241 cagaagcccg accgtgaggg agggcgtata gctccggcgt catgcgccct ccctcacggt
     3301 cgggctactg cctgcgtcct ctgcggtgaa ctgactaccc tacactccac tgaaaattgc
     3361 tatagttaat ttcaggacaa tgcagaccga cgtgaaaaca gagccgagtt ctgggggcgg
     3421 gacgcagcag cagcgcgccc cgcagttgag cgtggtgatg cctgtgcgca acgggctgcc
     3481 gtttctcgac gacagcgtcc gcagcatcct cggccagacg ttcgccgact tcgagctaat
     3541 cgtcttagac gacgcctcga ccgacgctac gctcgccgcc ctgcgcgaat gggagcgccg
     3601 ggactcgcgc gtcagggtct acgagagcgg gcgcaagctc ggtctctcgg gcagctcgaa
     3661 cttcgttgtg gagcaagcgc gcgcgcccat tgtcgcgcgg atggacgccg acgacgtggc
     3721 gcaccccgaa agactcgcgc gccagttcga ggtgatgcgg ggccggcccg aggtcgtgct
     3781 cgtcggcgcg ctcagcgagg gcatcgacgc cgacggccgg agcgtgcgcc cgcgcgaccg
     3841 ctggcggctg ctgcgctcct cgcccttccc gcccttcccg cacggctcgg tcatgttccg
     3901 ccgcgcggcc tttgacgccg tcggcggcta ccgcgaggag tgcgtgaact gggaggatca
     3961 agacttcttc ctcaggctcg tgcgccgggg ccgcgtcgtc gtcctcccgg aaaccctcta
     4021 ctactatcgc taccacctca ccaactcgac gggcggcctc cccgtcgaac acctcgcgca
     4081 cctctatgcc atcagaagcc tgtgtctcga agagttccgc acggggcgcg actacgaggg
     4141 cgtgcttgag caggccgccc gctccactcc aaacggctcg gggccgaagg ctgcggccga
     4201 cgcgctctac cagacgggcg cgatgcgtct gtgggccggt cgagcgcccg ccatcctcgg
     4261 cgacactctc cggcacggcg gcctgcgtct gggtgggcgg agcctgatgg cgctggtgct
     4321 gggcgtgtgg ggcggcgtca gcccggtgag tctgcgcgcg tctctgcgct cgttcatccg
     4381 cgcgcgcgac ctgttggcgg gctggcgcat gaaaaacggg agcccacgcg aatggcgctt
     4441 aaagtgaccg tcgtgggcct cggcggccgg ggccgacagt gggcgcgcga ggtgtcggcc
     4501 gcaccgggct tcgagctggc ggcgggcgtc gagccggacg gcgaggcgct gcggcgcgcg
     4561 cgctccgagt cggggttgcc atcggagaag tgcttcgcgg atttagacga ggcgcttgag
     4621 cggactgcct gcgacgccgt catcgtcgcc acgccggcgg agcgccacgc ggccgcctgc
     4681 gaggcgacgc tcgcgcgcgg cctcgccgtg atggtcgaga agcccttcac gatgcgtctc
     4741 gccgaagcgg tcaggatagt cgaactcgcg gagcgtaaac gcgctccgct catcgtcgcg
     4801 caaaactatc gctacatgcg cgcgttccgc acggcgcgtc gcctcgtccg ctccggcgcg
     4861 ctgggccgcg tcgggtcggt cgtctgccag tactaccgcg tgccgcacga gatggcggcc
     4921 tcgctcgccc gactcccgca ccgagtcctc tggggcatgg gcgtccatca cctcgacgcc
     4981 ttgcggcacg tgctcggacg ggaggcgacg ggagtctgcg ccgagagcta cacgacggcg
     5041 tggggcgagc cgcccgaggg cgcgtcgatg cgcgcgacgc tttcgttcga gggcggcgcg
     5101 cgcggcttct actcggccac ctacgagtcg agcggccacg aatacttcga gcgcggtcag
     5161 gagttctacc tgcgcttcac gggcgagacg gcgacgctcc acgtcttcca ccgctggctg
     5221 gtgttgtgcg agcgtggacg cctgccgcgc cccgtccgcc gcggcccccg ccgcgtgtcg
     5281 gaggagcgca tcctcctcga tcaactcgaa ggcgcgctgc tcaggggcga agagccggac
     5341 gcgagcgggc gcgacaactt acagacgatg gccattgtcg aagcgtgtct gcgctccgcg
     5401 gccgagcggc ggtggattaa tccgcaggag ttattagatg aacaggggta ggcaaacgcg
     5461 tgtgctcgcc gtcggcatcg acgccgccga gccgacgctc gtccgcgagt tgatcgaacg
     5521 gggcgagatg cccgtcctca agagtttatt ggacgaggga gcgtggctgc gcgtcgagtc
     5581 gggcgcgcac gtcggcagcg gggcggtgtg gccgaccttc atcacgggcg caagccctgc
     5641 cgagcacggc atctacggcg agtgggcgtg gcagcccgag acgatgagcc tcgcgcgctt
     5701 caacgcgcgg ggtctcaagc cgttctggga accattagcc acgcgtgacg gtctgacggt
     5761 gggcgtcctc gacgtgccct tcggcccgct cgtcggtctt acgcgcggct ttgaaatctc
     5821 cgagtggggc gcgcacgaca cgctggaagg gcggacggag ttcgcgcccg cctccctgtc
     5881 cgccatcctc aacgggaaga cgggcgcgca cccgttcacg cagaagcaca tcgaccccgc
     5941 cggcccgatt gattatcccg agatgagaaa tttgagcgcg gcctgcatcg agggggcgcg
     6001 gctcaggggc gcgctcgccg aacagttgat acagcagacg cggcccgact tcgcgctcat
     6061 caccttcacc gaggtgcatc acggcgcgca ccacgcatgg cacaccgtcg cgcccgaaca
     6121 tgagctttac gaagccgact ctttccgctc cttgcgcgcc gtcgagccga cgctcatcga
     6181 cctctaccgc gaagtagacc ggcagacggg gaggctcgtc gccgcggccg gcgacggcgc
     6241 gagcgtcttc gttttctcac tccacgggat gcggccctcg cgcggcctcc cgacgctgct
     6301 cgcgcccctc atgtgcgagc tggggtgggc gcgccgcgcc gacctctcga cgcagtcgtg
     6361 gacgggccgc gcactgtcgc tcgtggcggc cgtcaagcgt cgcatgccga aggccatcaa
     6421 gaagctctac taccggacga tgccgaaagc cgcgacctac cggctcgccc agccgacgat
     6481 gatgccggcc tacgactggt cgcgcacgcg cgccttcccc gtgcacaccg accagcacgg
     6541 ctggattcgc gtcaacctcg ccggccgcga ggcccgcggg ctagtccccg tcgccgagta
     6601 cgaagagacg tgccgccgtc tcgaagacgc gatgcgcgca ctgacgacgg aggacggacg
     6661 gccgctcgtg cgcgacgtga tccgcaccgc gccgcgcacg gaagacgccc tcgccctgcg
     6721 cctgcccgac ctcatcctcc actggcacga cgccgccttc gactcacccg tgcgcgtgca
     6781 gggcacgagc atcgaagcct atccgacggg cgcgaagttc accgggcaac acggactgga
     6841 aggcttcctt atcctgagcg ggcaagcgtc cacgcccgcc ggcgaccaca tcgacgggag
     6901 agacctccat cgcctcatcc tcaacgccct cggtgaccgc cgcgcctcga aaacttaaag
     6961 ggacgtaaca tcctcgatga aataaagcca ctgcatagtc gtcctttcaa tccgcgttca
     7021 cacattcaga ggcgtgctta gccatgtccc gtctcttcga cggaaatcaa atacgaaaca
     7081 tcgtcccccg gctctggagg cgcaccggcg agcagcacct ttaacaccgg cggcgcgatg
     7141 atcgaggtca gtatcgacat cgcgataatc accgcgtaga tagtttccga gaacaccccg
     7201 gcctgttgcc ccagactggc aacgatgatt cccacttccc cgcgcggaac catcccgact
     7261 ccgacaatca gcgccgattt tttacctaac gacagcgccc cgaggccgca acctacgagc
     7321 ttgctgacaa ccgcaagcag tgtgacaacc gcgaccatac ccagcaccga gaagctcccc
     7381 aactgtgtca cgtccacctt cgcgccggtg acgacaaaga agaacggcac gagaaacgcc
     7441 atgatgggct gaaactgctt ttccaaagta tgcctttggc tcgattccgc catgaccatc
     7501 ccggccagaa atgcgccgat gatggccgcc agccctatcc acgcggccgc cgccgccaga
     7561 ccaagacaca aggccagaga gagcgtgagc ggggagagtg gattgatcgg agcttcgagc
     7621 agattggacg accggcccat aacgcgcgtc cccacgagcg cgacaagggc gacaaagccg
     7681 acggcttgag caagaatcaa cagaagtccg acgacattaa caccgcctcc cccctgcacg
     7741 gccgtcacga caccgaggag aagcatggcg agaacatcat caatgacggc cgcgcctaaa
     7801 atgacgcggc tctccgtccg accgaggacg cccaggtctt gcaacacgcg ggcggtgatg
     7861 cccgcggaag tggccacaaa ggcggcggcg atgaacatcg ctttcggtgt ctcgtatccc
     7921 gaaagccagg cccacagccc accgagcaga aaaggcagaa cgaccccgag caccccgacg
     7981 aaggaggcga cgcgcccgac cttccgcaga tcaccgacgc gtgtttccag cccaacggaa
     8041 aatagaagta gtaccgcccc gatctccgcc aacacctcta aaggctcgtt gagttttatc
     8101 cacccgagag catatgggcc gattaagcag ccggcggcga tctcgcccac tacggcgggg
     8161 agctttattc gttgggctat ctctccgcct atctgcgcgg caacgaagac cacgaaaagg
     8221 gagagtagaa tttccacgcc atgatccaag gctctcggct cctggttgag tttgataaga
     8281 gggacagagt agttttggca cgcgcgcttt acaactcaaa tcactatgca ctgaaagtgt
     8341 atggtgattt gagttcctta acagcgggca cactataggc acacaaagta agaaagggca
     8401 gccccgcggg gctgccctta acttatggag tgggttgaac tgggaggcag ggactcgaac
     8461 cccgatagcc agatccagag tctggagtcc taccattgga cgacctccca aaaagtcggc
     8521 gagagaaaat ttacgtttgc gccgggcgac ttgtcaacca cggcccgcgt cgtcttcggc
     8581 ggccaagccc ttgccctcgc aaggatgtgg gcgcacaatc agagtgcgtt gtgtggaact
     8641 ctgaagaggg cgcgcgccga gacactcacg cgcgcacgcg cccggcggcc cgaaaaccca
     8701 cctcggccgg agggtaaacc cgtgggcgac gacgcatcgt taaaggaaga agtggagcgc
     8761 ggccttgaag gggagccgcg cggacgcgcg gacgcgcgcg atgagccggc gcgcttaacg
     8821 gcggagcttg ccgcgcgcgt ccgacacgag atcaacaatc ctctgaccgg cctcatcggg
     8881 caggcgcaac tgctgctgcg cgacgacacg ctgagcgaga cggcgcgccg ccgtgtcgag
     8941 atcatcgagc agctcgccaa gcgcatccgc gacgcggtcg cggagttgcg cgccgtgcga
     9001 aatccgccgg gcggcgcggc cggcgcgggc gcgcggggcc ctggcgagac cccgcgcgtc
     9061 gaagacccgc cgcgccacta aagccaccga aacgtccttc aatcttccgg ccccgacgcc
     9121 gcgccgctca ccgtcgcttc ggcgcgcacc gacacgtcga aacgcttgcc ctcgggcaga
     9181 tacgcgttct cgccgcgctt gaagccgtgg agcagcgcga ccggcgcgac taaggggagc
     9241 aatgcggcag tgagcgcaat ctgcgtggcg accttcgcgc ccttgctgtc gccaacctgc
     9301 cggccggcga actgtatttg aagccgcgac ccgtcggcgg cggtcacctc atacatctcc
     9361 caggcgagcc ggccggcgcg cccccagtgt ccgccgcgcg aagccttgac gacgcgcgcc
     9421 gtggccaccg cccccggctc cacgaccgtc acgccgccga ccttcacggg attgaccacg
     9481 cggaaggtta tcaggtcgcc cacgcgcacc tcctgcgagt tgaccctgta ggccgactcg
     9541 acttccacgc gcgtccccgc cggcaccttc accgcgacga cctttttcga ctctcccgcc
     9601 ggtgcagcca cagcggggac gcgtggagcc tcgaccacgg cgaggatgac ggagtcgggc
     9661 gcacccgcgt cgcgcagagc cttgagcgcc tcgggcgacg tgtcgaagtc ggcggccgaa
     9721 gtcttaatct tggcgacgac gacctctggc gcgagccgcg cttcgagcat cttcagcacg
     9781 tcgcggttgt caatgcgggc cggcgcggcg tctgcggtgg cggaggccgg cgacggttgg
     9841 acaattttcg cggcgtcctg gccgaacgca gccaccggcg agagggccgc taagattatc
     9901 aacagtaatg aaattggccg gggaaattta ttcgtcatgt gagtggtctc ctgtcttcat
     9961 caactaaaac aaagaggggc gtggcaacat gaatgccacg ccccttttct tctggctcag
    10021 ttgatttaga cagagacgac gagcactgcg caggcgcgcg tccgtttgtc gcgcgcttct
    10081 gactacgacc cggctactgc cgatgccggc ttgcccttga tggcggtgtc gccatgcacg
    10141 aacacgcggt agcggttgcc ggcggggatg atggccggct tgcccttctt cagaccccac
    10201 aggaggccga tggggccgag cagtacggtc gtcgccacgg cggccacggc caccgtcccg
    10261 cccttgctgt cgccgagagc gcgatccgtg aagcgcatgg ggacgcggtt gccgtccgac
    10321 gcctgcacgt cctgcatcgc ccattcgagc ttgccagccc gcccccaacg cccggccttc
    10381 ttggcggtgg tgatgcgcgc acgcgccgac gcgtcgcgct caaagatggt cacgccgttc
    10441 agcaccacgg ggtgtaagac cttgaaatcg acgaaggagc caaccttcac ctcttcacca
    10501 ctgacgacgt tgattaactc gatctcgacc tcggtgccgt ccgggacttt gatctcgacc
    10561 ggcgcggctg ccggggcagt cgtcatcgtc accgcagcgg ggtcgccggc cggcgcctcg
    10621 accattgcga ggatgacggc gtcgggcacg cccgcagcct tcatctcctg aagggcggcg
    10681 gccgaggtgt cgaacgctgt ggccgacgac ctgatcttgg cgacgacgat ttcggccgac
    10741 aggccggcct tgagcatttc ggccacgtcc ttgttgatta gcttggggga gggcggctgt
    10801 gtctgttgtg cctgactcgt ctgttgctgc tcctgctggg ccgcggccat gatggcggcg
    10861 gcgggggaaa cgagtgccgt cgtgagtgcg agggacaaaa atctattcac gttggtaaat
    10921 cctttctgac tgattagtga ctttgtgatt ccgctttcgg gggaaagcgt cgggcgtcgg
    10981 gatgggaact gtcgagttgg gcggcgcggt cgggtgaccg ggactgaaac ttcgcggccg
    11041 ggcgccctct tgaatcggcg ggcgcccggc gtcttaaaaa atactcttac gacacggccg
    11101 cctcttcttc ttcgacgaga tacggagtcg tcgcggcggg cgcatcatct tcgatgatgg
    11161 cctgaatgat gggaagcgag aggccgcgcg acttctggat gccggggacg tgcttgccct
    11221 tggcgtagac ggcgtggcgt ccgtgctcct gataccactc gccgaggttc gccgtcttgt
    11281 gcatgttctc gatgatctgc cgccagccga ccccggtgtt gtaggcgcag aaactaatct
    11341 cgccctcctg cgtgccgtag gggatgacgc acatctcggt gcggcggaag tcgtagttga
    11401 ataaatcctg gaaccacatg ccttcaacgc agagcacccg ccacacgtcc gaggggtcgt
    11461 ctgctccctg cgacttcatg cggtcgttgc ggtcggagtt cgagctggtc gaagacggct
    11521 tgaagagatt gacgatctgc gagacgggga agccctcggg cgcgcgctcc gctttgaagt
    11581 tgcgcatgat ggccatgccg agctgcgcgt aggagagctt gcggccccgc gccgtgtccg
    11641 tgatgacgcc cacgtccttc atgaactgct cgtagtcgaa gaactcgaag agggagcgga
    11701 attcgcccgt ctggcggttg acgaccatca gggtgaagac gccgcagttg gggtggcagt
    11761 tgcacgaaga ccagccccac ggcgcgtccg cgccttgcag catatccatc accgaggtga
    11821 aggccgagta ggaggagagc gggaaccagt cgcgcagcgc ctcgacacgg ccgccgagct
    11881 gcgactccag atcgccggcc atcccggcga gcgtgtagcg ttgctgcaag cggatgttat
    11941 cgggcacgtc ctcgtcgcgg ccggtgaagg agacgggctg gaaggcgatg gtctgcacct
    12001 tgtcgatgtt cttcgccgcg aactcgacga tctggccgat gccgtcgttg ttgatcgtgt
    12061 tgacgatggt cgtcaccagc gtcaccttga tgccgaccgc ggccaagttt tcaatcgccc
    12121 gcagcttcac gtcgaagagg ttgccgacgc cgcggtgctt gttcttctct tcgctgacgc
    12181 cgtcgaactg caagtagacg ccgtgcatcc cggcatcctt cgcctgctgg caatactcga
    12241 cgctctcggc gaagcggatt ccgttggtgg ccgccagtat ccggtagaag ccgatcttgc
    12301 gggcgtagga gaccgcttcg aggaagtggg gcgagatggt cggctcgccg ccggagaaga
    12361 ggatgatgat ctgccggcgc ggcttgaagg agacggcgcg gtcgaggatg gctttggtgt
    12421 cgtcgagcgt cggctcgtga acgtagccga cctgattggc gtccatgaag caggggttgc
    12481 acatcatgtt gcagcggttg gtgaggtcta ccgtcaacac ggcgccgcgg ccgaacttga
    12541 tgttggaagt cccgtgcttg tggatgtgcg cgtcctcggc ggcgcggaag tcgcgtccga
    12601 agaagagcga ctcgatgcgc gcgaggaagc gcgagtcaga ggccatcagg tctttgaact
    12661 cgccgtggct ggggcaggtc ttcgtcatgt agacccgtcc gtcctcctcg atgatctgcg
    12721 ccttgatctc gccggggtgc gtgtgcatca aggtctctaa cgacacgtcg ccggagatga
    12781 cggcgttgcg aacctctttg acacagctcg ggcacagaga gtcggtctcg cgcgggaagc
    12841 cgagcggcgg gctggtgcgc tcatacgact tcaggagcgg ctcggcggcc cacttcggat
    12901 gaatcgccgc gccctcgggc aggcggcggt tgaccgattg gaacgtcttc caggccacgt
    12961 ccgaaaggtt cgagatgagc ttcgaggtct tcacgccgcc gaagcgcgac ttcactgtct
    13021 cgccacggcg gaaattgtcg ctgaacaagg ctttgtactc tcctcctcgc ggaagcgcgc
    13081 gcccgcaaat gcttgaagac acggccgctt ggaatcttcg ggaaaattaa caggagctac
    13141 tgtctgccac gggccggacg cgccgaccgg gcacactttt gcgccgccac atgataccgg
    13201 catccttcag cgagtacaag gcgttacaca gacgtgaagg caaaagtaaa aaggcaaaag
    13261 gcaaaagtga aagagcaggc cgcctcttca cttttgccct ttaccttttt acttttgcct
    13321 tttcacggat gtaccatccc cggcgagtca acgcgcagcc catgcaagca cacccgacgg
    13381 ccatcatcca cccgcgcgcg caactggcgg cgggcgtgcg cgtcggcccc tacgccgtcg
    13441 tcgaggagga cgtgacggtc ggcgcggggt gcgacatcgg cgcacacgcc gtcgtcaaga
    13501 ggttcacgac tctgggcgag cgcaaccgcg tctacgagca cgcggtgctg ggcggcgagc
    13561 cgcaggacgt gaaattcggc ggcgagcgaa gtcatctcca catcggcgac gacaacctca
    13621 tacgcgagta cgcgacgctg caccgggcga gcggcgaggg cgcggcgacg cgcgtcggct
    13681 cgcgcaactt cctgatggtc ggcacacaca tcgcgcacaa ctgcgaggtg ggcgacgaca
    13741 acatcttcgc caacggggcg gcgctcgccg gccacgtccg cgtcgaagac cacgtctttc
    13801 tggcctcgaa cgtcggcgcg caccagttca cgcgcttcgg gcgctacgcg atggtcggcg
    13861 gcaagtccaa aatcattcag gacgtgctgc cgttcttcac caccgacggg aaccccccgc
    13921 gcgtgcgcgg actcaacacc gtgggcctgc gccgcgccgg attctccgcc gagtcgcggc
    13981 gcgaactcaa gcgcgcctac caactcctct tccgcgcgcg cgcgcccctc gaagaggtgc
    14041 tccgcgaaat ggagcgaacg gacgacgaac acgtgcgcca cctcgccgcc ttcatccgcg
    14101 gctcgcgccg cggcttctcg cgcgagcaga ggcgcggacg agagacggcc gaggaaactg
    14161 acactgctga agaatgatgc ttaaaggaaa aacagtcgcg gaactcgccg cgctcgtcgg
    14221 cggtcgcgcc gtgggcgacg ggcaagtcgt cgtcgagagg gtggcgagtc ttgaaggcgc
    14281 ggggcaggga agcgtcgcct tcgtcgcgga cgcgaagatg atggaggcgg cgcgcgcgag
    14341 cgacgcgtcg tgcctcatcg tgccggaggg cgcggggccg gaggggcggt gccgcatcga
    14401 ggcggctaac ccgaagctgg ccttcgcgct cgtcgcggag gtcttgcatc cgccgaagag
    14461 gaacgagccg ggcgtgcatc cgacggctgc ggtcgagcct gatgcgcgtc tgggcgaggg
    14521 cgtgttcgtc ggcgcgggcg cgcgcgtcgg cgcgggggcc gaggtcggcg cggggacgca
    14581 gattcaagag ggctgtgtgt tgggcgcggg cgtgcgcgtc gggcgcgact gcgtgctgca
    14641 cccgcacgtg gtgctctacg agggcgcgac gctcggcgac cgtgtggttc tgcacgcggg
    14701 cgcggtcgtc ggcgctgacg ggttcggcta tgtgcgcgac gtggcgggcg tctaccataa
    14761 gtttccgcag gtggggacag tcgtcatcga gtcggacgtg gagattggcg cgaacagttg
    14821 cgttgaccgc ggcgcgctgg gcgagacgcg tgtcggccgg gggacgaaga tcgacaacct
    14881 cgtgcaggtg gggcataacg tgtcggtcgg tgagcgcgtc gtcatcgcgg cgcagacggg
    14941 gatttcgggg agcacggtga tcgagtctgg cgccgtcatc ggcgggcagg tggggatggg
    15001 cgaccacgcg cgggtggaga gcggcgcggt catcggctcg caggccggcg tgctgccggg
    15061 caagatcgtg cgcgcgggcg tgtggtgggg cacgcccgtg cagccgctcg aagagtacaa
    15121 gcgcctgcac gcgctctggg gccggctgcc gcgcatgcgc gaagagatca aagagctgcg
    15181 cgcacagttg aaggaactgc aagcgaggat gggcgagaag gcggattaac gattcctgat
    15241 gaagcgttcc ggcgacagtt gagtgtcagg cagggcagct caccgcagag gcgcagagag
    15301 cgcagagttc acgccgcaga gttgaactac tcaattattc ctgagacgcg tcgatgaagc
    15361 gcgcgcggac ttcgggcggc ggcagcaggc aggtgtcgta cttgccgaac attttgtagc
    15421 ggtggcgcgc gaagaggtcg tacaggtaat cgcgcacgct cgcgggcagc agccggccga
    15481 gcaaaaaaat cttccacgcc ccgcccatgt aggacgcgac tctcagcccc gcctccgagc
    15541 gcacggacac gcgctctctg cccgtctcag gcgacgcctc caggaacacg accgaatcga
    15601 cgcccgcaag ctccgggtgg cgcgcgaggg ccgcccggcc gtagtcgctc tgcaaggcgg
    15661 cgaagcgcaa gctgccacgc cggtcgtggc gcaggatggt ctggacggcc ccgttgcaga
    15721 agccgcacac gccgtcgtag agcaacacag gcgcacgggg tgtgagagtc tcagtcaccc
    15781 ttcctccttc caagcgcgaa ccgcgcacaa caccgcgtcg gccgcgcgca gggaagcgcc
    15841 gccttcaccg agccgagcca cagcggcgcg caggcgcgcg cgcacctccg agttgcgctc
    15901 gtcgtcgagc agcgccgaga gttcgcgcgc gaggcggtcg cccgtgaagt cgtgctgcat
    15961 cagctcgggc gcgacgcgct cacccgcgat caggttgacg aggccgtagt gctcgacgct
    16021 gatgagccgc ccgagcgtgt tccagttgac gaacgactcc ttgtagacga cgaccgtggg
    16081 cacgcccacg agcgccgcct ccagcgtcgc cgtgccgcta cagacggccg ccgcgtccgc
    16141 cgcgcccagc gcctcacgcg tctcgtgctc gacgaagagc agtcggcccg caacgtccgc
    16201 gccgccgggg acgttcgcaa ttaatgcctc cgcctccgcg cgcgtgcggc gtgcggcgag
    16261 cgcgatgacg aactgtgtgc cggggcgtgt gcgcgcgagt cgggccgtgg cgtcgagcat
    16321 gacggggaag atgcgctcga aatttttgcg ccggctgccg ggcaggagcg cgactatggg
    16381 ccgcgacgct tcgaggccgt ggcggcgaca gaattccgcg cgccccgcgc cggggcgcac
    16441 ctcgccggcg agcgggtggc cgacgaactc gaccgcgtcg aagccgcgcc gggcgtacca
    16501 gtccttctcg aatgggagaa tcgtgagcaa caggtcaacg tcgcgtcgca cctgccggac
    16561 gcggtaggag cgccacgccc agagttgcgg gctgatgtag tagacgacgc gcaagccacg
    16621 gcggctgagc gcgcgtgcga gccgcaggtt gaagtcgggc cagtccacga gcacgacggc
    16681 gtcggggcgg cgctcgacgg cggcccggcg gagtgcgcgg taggcgtgcc agaagcgcgg
    16741 gagcgcccgc ccgatttcca gcagacccgt gatcgccaga tcgtcctcgc gcacgaccga
    16801 ctccacgcca gcctcgcgca tccgccgtcc cgtcgcgccg aagaactcga agctcacgcc
    16861 gggcgcgcgc tcgcgcagag cgccgaccag cgccgccgcg tgcgcgtcgc ccgagtcctc
    16921 gcccgccacg atcatcaggc gcagtcgctc tgctgcgggg ccgtccgtca tcgggcggat
    16981 gatagcagta gtcagccgtc agctattagc tgttagctgt tagctttcag ccagaggccg
    17041 ccgccgacac tcgacaactt ggttcgctct ctaacagcta acagctaata gctaatagct
    17101 aatagctcat ttataactct gccctatggc ccgcccgaag ctgatctcgc cgttatcaat
    17161 tcgtagattg aacccgcagt cgggattggt gcagacccag gccttgtagg tgatgggcgc
    17221 gccttcacgc ccgtagtctg atagcgggat caaggtgccc tgcgagcact tttgacagcg
    17281 tggccactct ttggcttcca tca
//
LOCUS       DASVES010000236        11367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_6445, whole genome shotgun sequence.
ACCESSION   DASVES010000236 DASVES010000000
VERSION     DASVES010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6445"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1531)
                     /locus_tag="VF240_09620"
     CDS             complement(77..1531)
                     /locus_tag="VF240_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7175503.1"
                     /translation="MPSMKKIQQALCALVACAVLFGGAACVQNSETGGTVASNARPAN
                     SNAAPAASPQVATNAAASNAGQMPVTLAVLDALFAEASFAGELKTRLGLTDEQLERLK
                     AAAREEREGLAESEGDAEHAGTTSEASARATEKIRAAVGPEKAEQLMAFARERWSTGG
                     EAGAGGAAAAGAVDAAASKPNAVPTDTRVVVNIPAYRMDAFEGGQLVKTYKVGIGYPE
                     FPLPTGLRKASEIIFNPTWTPPDEPWVKGKFKPGEKVDAGSKDNPLGLIKIPIGSPSL
                     IHGGKSPARLGTFASHGCVGLTNPQVQDFATVLAKLGGAQITREQVLTYAKVREETKN
                     VKLSNNVPVELRYETIVVEDGKLHVYRDVYGHGTNTEENLRKTLEAYGVRLEDLSEPE
                     RAQVKQALTEMARDALGKPATEAQPTPTPARKGAAASARETKTMKGKKEIVVEIAALR
                     GKGYPAPANFDAGTGAPPKSAPQKAAQTSAKPQA"
     gene            complement(1812..2696)
                     /locus_tag="VF240_09625"
     CDS             complement(1812..2696)
                     /locus_tag="VF240_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7175504.1"
                     /translation="MKDEQAEREGTATDSAPPAGATDAARVGDAGRTGAASGAWRRRA
                     RLKVLLLALLALAVVGALVWYLSVRYAATPVEPITTRPAANAEATPVSATPVATVIPS
                     PEIPAPTTSPTPLVEASPSAAGQQPPGGLSPSPAPPASSDGTQVRLSIPVAGVRPEDL
                     RDTFTQSRSEGRTHDAIDIMAPRGTPVVAAADGPIVKLFQSVPGGITAYQLATDNRTV
                     LYYSHLERYADGLAEGHFARRGEVIGYVGDTGNAGAGNYHLHFSVSITNDPKRWHGGT
                     NINPYPLLRGQRLEVRGQ"
     gene            2903..3232
                     /locus_tag="VF240_09630"
     CDS             2903..3232
                     /locus_tag="VF240_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX7175505.1"
                     /translation="MADAFDDRRKALEEDYFHRKDRETLARLRESMGEAAKARGEAAA
                     TLACPRCDGRLHEVVFDDISIDRCDRCEGVWLDAGELKHIIGKGQSSGGWLSALWPGR
                     AGKQTTE"
     gene            3333..4124
                     /locus_tag="VF240_09635"
     CDS             3333..4124
                     /locus_tag="VF240_09635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7175506.1"
                     /translation="MSEQRLTALVTGASGGIGEELARLLAADRHDLVLVARSRDKLAR
                     LAEELGGKHDISARVIARDLAFPASPREIFDETEGAGVRVDVLINNAGFGSYGPFAET
                     DAEKELEMLQVNVVALTHLTKLFLPAMIARGRGRIMQVASTAAFQPGPLMAVYYASKA
                     YVLSLSEALSNECEGTGVTVTALCPGPTETGFVAAAGMEKSKLFEANVMDARPVAVAG
                     YRGMLDGRAVVIPGFRNSLMARAVGFAPRSLVTKIVRRVQETRNK"
     gene            4213..4731
                     /locus_tag="VF240_09640"
     CDS             4213..4731
                     /locus_tag="VF240_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX7175507.1"
                     /translation="MEDDKLAADEARREMQHESVKAQVEADVNSEIAARAERTTPVEA
                     RKMETVAGEFRGKAIDEVVETEREVESARTMARVSQVVDYVFYVIYGLLAIRLLLALL
                     AARSRAGFTQFIYAVTDPLYAPFRGIVAEPTVEGGYRLALSVVIAIIVYALLHAGING
                     LLRLFAHRKTRV"
     gene            4843..6510
                     /gene="hutU"
                     /locus_tag="VF240_09645"
     CDS             4843..6510
                     /gene="hutU"
                     /locus_tag="VF240_09645"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX7175508.1"
                     /translation="MSRPRNIRAPRGAELNCKGWHQEAALRCLMNNLDPEVAERPDDL
                     VVYGGRGKAARDWNSFDAIVRELTALENDETLLVQSGKPVAVFRTHEYAPRVLIANSN
                     LVGAWATWEHFHELERRGLMMYGQMTAGSWIYIGTQGIVQGTFETFAAMADKNFGGTL
                     AGRLVVSGGMGGMGGAQPLAATMNGAVFLGVDVDRERIERRVRTGYCDLLAVSLDDAL
                     RICADAVEQQRAVSVGLVGNCAEVLPEIVRRGVKVDAVTDQTSAHDPLNGYVPAGLTL
                     EEADELRQRDARVYVERSTESMARHVEAMLELKRAGAVAFDYGNNIRKFAHDAGCRDA
                     FEIPGFVPEYIRPLFCEGKGPFRWVALSGDPADIRRTDDLALELFPEDRTLDRWLRLA
                     RERVQFQGLPARICWLGYGERAEMGEAINDLVRRGELQAPVAIGRDHLDTGSVASPFR
                     ETEAMRDGSDAVADWPILNALLNTASGASWVSFHHGGGVGIGYSLHAGQVSVADGTPE
                     ASRRLNRVLTNDPGLGVARHVDAGYDEAEETARTKGVKIPMLGAGDE"
     gene            6687..6971
                     /locus_tag="VF240_09650"
     CDS             6687..6971
                     /locus_tag="VF240_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175509.1"
                     /translation="MADEKDPTASGQPSSPEGIIVPDAGNPSESQEGIIIPQAGGPSE
                     STEGVIVNQAEQPGEASAAGAAAGAGGATGTASSEEGDEPPQKDGVIMEF"
     gene            7240..7854
                     /locus_tag="VF240_09655"
     CDS             7240..7854
                     /locus_tag="VF240_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175510.1"
                     /translation="MKHRSRLIALVALLFAAAIPASANIAPPNVPTKPPRQTTGAPVR
                     MQLRPDADAKEAKLIIPRKVLQQLKAELDGGADPQTAAAAVASSGSNLSSTQTAVAGV
                     FLSLSLVLGGVWLAGSRAPGRRVSRVVSALAFAALGGAAASGVAVYANAGPPPVARSL
                     TSKILAPDLSYWGASGVVKIEIANDESLIMLVLPKAKDAATAGE"
     gene            7861..8895
                     /locus_tag="VF240_09660"
     CDS             7861..8895
                     /locus_tag="VF240_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175511.1"
                     /translation="MRALAAQANATVTLVGHEALAPRFGVPFLGFCLLAALLVSWWPL
                     QLSIATVFLFAGPHNWMEFRYFLSRMPARWGRSRSFFAVGLGGTLLLTAAYTVLFWLG
                     QTWYLSEAAWTAGVSLWGTALLVWACALVWLRGRRRAARGDYLPVYAAGSALAALAWV
                     APQWFFLALVYAHPLVALWFLDRQLKRTRPHWRKTYHLCLAALPLLLLLMWSRLAGAP
                     DLAETDALSWRITRHAGASILPGVSTHLLVATHVFLETIHYGAWLVLIPLAGLQRAGL
                     FETRRIPLAVHRRGGWPRAVKASLLFGAFVVVALWAFFWADYTTTRDLYFTLAMGHVL
                     AEVPFLLRLL"
     gene            8991..9449
                     /locus_tag="VF240_09665"
     CDS             8991..9449
                     /locus_tag="VF240_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175512.1"
                     /translation="MELQVAIYDLMIFDIAPPPVVTSRPDELWYFLPFGYLLTVAVET
                     PVLFFGLSKRLTRWQRLFAGLWLTACTYPVVVLVLPALFATSSRGLYLLAAETFAPAA
                     ECVIFWLAIGRRLDVSARAKLLNFATIVAANILSFAAGEVLGATRWFGLF"
     gene            9536..10918
                     /locus_tag="VF240_09670"
     CDS             9536..10918
                     /locus_tag="VF240_09670"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7175513.1"
                     /translation="MDEEPTHVETHLTPDPVAAAEPRAVGEGGRVRRAARALAIDITP
                     LKVSRDFRLLFFGQTVTFFGSMMTFVVLPWQMYRLTGSSLAVGMLGVAEFVPILLMAF
                     VGGALADYFDRRRMVLLTELALAAGTAVLILNSLLPQPRVWVLYVCASLFAALNGLQR
                     PSLDSLIPRLVPAELLTSAAALRSVGLTAGMIGGPALGGVLVAAFGPVVAYSIDFATF
                     AASLAALAMLRAVPPPPGADRPSVRTVVEGLRYARSRPELMGTYLVDINAMFFGMPMA
                     LFPAIGESLGGPKVVGLLYAAPAAGALLATLTSGWSKHVNRHGLAVCLAAAVWGLAIV
                     GFGFASSLWLAVLCLAVAGAADMVSGLFRMTIWNQTIPDHLRGRLAGVEMVSYTTGPL
                     LGNAEAGIAARLFGVRASVVSGGVLCVVGTGLLALLLPAFLRYDGREGLKRKAAEEEA
                     RAALLGEASE"
     gene            11026..>11367
                     /locus_tag="VF240_09675"
     CDS             11026..>11367
                     /locus_tag="VF240_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175514.1"
                     /translation="MRRTQLRRAWLAPLLLVVAFAATATHAQAVAPAAGTSVETINFE
                     SKLVGRRLPYKVVLPPGYNAAASTPPTLYPTLYLLHGLTGHHTDWLARTKLSDYAAQY
                     RLIIITPEGNDG"
BASE COUNT         1708 a         3888 c         3754 g         2017 t
ORIGIN      
        1 cgatatctaa acaacaacgc cgcaaccccc gtagggctgc ggcgttgttc gttccactta
       61 attactccct tctatctcaa gcctgcggct tcgcgctcgt ctgcgccgct ttctggggcg
      121 cggacttcgg cggcgcgccc gtgccggcgt cgaagttggc gggggccggg tagcccttgc
      181 cgcgcagggc ggcgatctcg acgacgatct ccttcttgcc cttcatcgtc ttggtctcac
      241 gagcgctcgc cgccgcaccc ttcctggcgg gcgtcggcgt cggctgcgcc tcggtcgccg
      301 gcttgccgag ggcgtcgcgc gccatctcgg tgagcgcctg tttgacctgc gcgcgctccg
      361 gctcgctcaa atcttcgagg cggacgccgt aggcttccag agtcttgcgc aggttctctt
      421 ccgtgttggt gccgtggccg tacacgtcgc ggtagacgtg gagcttgcca tcctcgacga
      481 cgatggtctc gtagcgcagc tcgacgggga cgttgttcga gagtttgacg ttcttcgtct
      541 cctccctcac cttcgcgtac gtcagaacct gttcgcgggt gatttgcgcg ccgccgagct
      601 tggcgagcac ggtggcgaag tcctgcacct gcgggttcgt caggccgacg cagccgtgcg
      661 aggcgaaggt gccgagacgc gccggggact tgccgccgtg gatgagcgac ggcgatccga
      721 tggggatttt gatgaggccg agcggattgt ccttgctgcc cgcatcgacc ttctcgccgg
      781 gcttgaactt gcccttgacc caaggctcat cgggcggcgt ccacgtcggg ttgaagatga
      841 tctccgaggc cttacgcaac cccgtcggca gcgggaactc cgggtagccg atgccgacct
      901 tgtaggtctt gacgagttgt ccgccctcga aggcgtccat gcgataggcc gggatgttga
      961 cgacgacgcg cgtgtcggtc ggcaccgcgt tcggcttcga cgccgccgcg tctaccgcgc
     1021 cggccgccgc ggcgccgccc gcgcccgcct cgccgcccgt gctccaacgc tcgcgcgcga
     1081 aggccatcaa ctgctcggcc ttctcggggc cgacggccgc gcgaatcttc tcggtagcgc
     1141 gcgcggatgc ctccgaggtg gtgccggcgt gctccgcgtc gccctcgctt tcggccagcc
     1201 cctcgcgctc ctcgcgagcc gccgccttca agcgttccaa ctgctcatcc gttagcccga
     1261 ggcgcgtctt caactcgccg gcgaacgacg cttcggcgaa gagcgcgtcg agcacggcca
     1321 gcgtcacggg catctggccg gcgttcgacg cggccgcgtt cgtcgcaact tgcggactcg
     1381 ccgcgggcgc ggcgttcgag ttcgccgggc gcgcgttcga cgcgaccgtt ccgccggtct
     1441 ccgagttctg cacgcacgcc gccccgccga aaagtacggc gcaggcgacg agcgcgcaca
     1501 acgcttgctg aatcttcttc atgcttggca ttccctctct gaactccaaa ctcttttcaa
     1561 ggcccgcatc tcgaaaggcg gccggcgccg ttcgcgccgg acgagactca cggaaataca
     1621 atacgcgtcg cggggccgaa cgccggccga cccgcctcgg cctagcctag caacccccgt
     1681 tccgacccaa ttatctccga aaatcagctc ttttcccccg tacccgcact ccccggccgc
     1741 tgtggcttgc aacataaccg aacagaggtc ggaggttaga ggtcagaggt cggtaaaaga
     1801 cgaattgctt tttactgacc tctgacctct aacctctgac ctctaagtaa tgggtacgga
     1861 ttaatgttcg tgccgccgtg ccagcgtttg gggtcgttgg tgatggagac ggagaagtgg
     1921 aggtgatagt tgccggcgcc ggcgttgccg gtgtcgccga cgtagccgat tacttcgccc
     1981 cggcgcgcga agtggccttc ggcgaggccg tcggcgtagc gttcgaggtg cgagtagtag
     2041 aggacggtgc ggttgtcggt ggcgagctgg taggcggtga tgccgcccgg tacgctctgg
     2101 aagagtttga cgatggggcc gtcggcggcg gcgacgacgg gcgtgccgcg cggggccatg
     2161 atgtcgatag cgtcgtgggt gcggccctcg gagcgcgact gcgtaaaggt gtcgcgcaaa
     2221 tcttcgggac gcacgcccgc gacggggatg gagagacgca cctgcgtgcc gtccgaagaa
     2281 gcgggcggcg cgggcgacgg cgacagcccg cccggcggct gttgcccggc ggccgacggc
     2341 gaggcttcga caagcggggt gggcgaagtg gtcggcgcgg gaatctctgg tgaaggaatt
     2401 acagtagcga cgggcgtggc gctcacgggt gtcgcttcgg cgttcgccgc cggccgtgtg
     2461 gtgatcggct cgaccggggt cgccgcgtat ctgacgctca gataccagac gagcgcgccc
     2521 acgactgcca gcgccagcag cgccagcagc agcaccttga gacgcgcgcg cctgcgccac
     2581 gcccccgacg cggcgcctgt cctgccagcg tcgcccacgc gggcggcgtc cgtcgcaccg
     2641 gccggcggcg cggagtcagt cgccgtccct tcacgctcgg cctgctcgtc tttcatctcg
     2701 ttagctgcac gtgtcctttg gccgcaagca tatgtggcgt ggcgcgtcga atcaagcgcg
     2761 cgcgcgtccg aggcgctggc gccgggccgc gtttttctgt tattacatag cctaaagttc
     2821 gcacgcgatt attcgaaacg agtgtgtggc gagggcgtcc gagcgccttg ggccgcgccc
     2881 gataaacccg gaggtgagga acatggcaga cgctttcgac gaccgccgca aggcgctgga
     2941 agaggactac ttccaccgca aggacaggga gacgctcgcg cgcctgcgcg aatccatggg
     3001 cgaggcggcg aaggccaggg gcgaggccgc ggccacgctt gcctgcccgc gctgcgacgg
     3061 gcgtcttcac gaggtcgtct tcgacgacat aagcatcgac cgctgcgacc gctgcgaggg
     3121 cgtctggctc gacgccggcg aactcaagca catcatcggc aagggacagt cgtcgggcgg
     3181 ctggttgagc gccttgtggc ccggccgcgc cggcaagcag acgacggaat aatgcaggga
     3241 gtcaggagtc aggagcccga agacagaatg aagaaagcaa gacggctttt attctgactc
     3301 ctggctcctg actcctgact tctttttttc ccatgagcga acagcgactc acggccctcg
     3361 tcacgggcgc ctcgggcggc atcggcgagg aactggcgcg gctcctcgcg gccgaccgcc
     3421 acgacctcgt cctcgtcgcg cgcagccgcg acaagcttgc gcgtctcgcc gaagagcttg
     3481 gcggcaagca cgacatcagc gcccgcgtca tcgcgcgcga cctcgccttc ccggcgtcgc
     3541 cgcgggagat tttcgacgag acggaagggg ccggcgtccg cgtcgatgtg ctcatcaaca
     3601 acgccggctt cggctcctac ggccccttcg ccgagacgga cgcggagaaa gaacttgaga
     3661 tgcttcaggt caacgtcgtc gccctgacac acctgacaaa acttttcctg cccgccatga
     3721 tcgcgcgcgg gcgcgggcgc atcatgcagg tggcctcgac ggccgccttc cagccggggc
     3781 cgctgatggc cgtctactac gcctcgaagg cttacgtgct ctctctctcc gaagcgctct
     3841 cgaacgagtg cgaagggacg ggcgtcacgg tcacggcgct ttgccccggc cccaccgaga
     3901 ccggcttcgt cgccgcggcc gggatggaga agtcgaagct cttcgaggcc aacgtgatgg
     3961 acgcgcgtcc cgtggccgtg gccggctatc gcgggatgct ggacggcagg gcggtcgtca
     4021 tccccggctt ccgcaactcg ctcatggcgc gcgccgtggg cttcgcgccg cgcagcctgg
     4081 tgacgaagat cgtccggcgc gtgcaggaga cgagaaacaa gtgataagtg atgagtgata
     4141 agtgataaga aaaggcggat gctctctctt atcacttatc actcatcact tatcactctt
     4201 aaggagatac tgatggaaga cgacaaactg gcggccgacg aggcgcggcg cgagatgcag
     4261 cacgagtcgg tcaaggcgca ggtcgaggcg gacgtgaact cggagatcgc cgcgcgggcc
     4321 gagcgcacga ctccggtcga ggcgcggaag atggagacgg tcgccggcga gtttcgcggc
     4381 aaggccatcg acgaggtggt cgagaccgag cgcgaggtcg agagcgcgcg gacgatggcg
     4441 cgcgtctcgc aggtcgtcga ttacgtcttc tacgtcatct acggcctgct cgccatccgc
     4501 ctgctccttg cgctgctcgc cgcccgcagc cgggctggct tcacgcagtt catctacgct
     4561 gtcaccgacc cgctctacgc gccgttccgc ggcatcgtcg ccgagccgac cgtcgagggc
     4621 ggctaccgcc tcgcgctctc ggtcgtcatc gccatcatcg tctacgcgct cctgcacgcc
     4681 ggcatcaacg gcctgctgcg cctcttcgcg caccgcaaga cgcgtgtctg aacgttaagg
     4741 ccgtgaatcg tcaatcgtaa atcgtaaatc ggagaatttt gtctttcacg attgacgatt
     4801 gacgattcac gattcacggc ttttaaggag tgtgtcaaaa gagtgagccg accgcgaaac
     4861 atccgcgcgc cgcgtggcgc cgagcttaac tgcaagggct ggcatcagga ggcggcgctg
     4921 cgctgcctga tgaacaacct cgacccggaa gtggccgagc gccccgacga cctcgtcgtc
     4981 tacggcgggc gcggcaaggc ggcgcgcgat tggaattcgt tcgacgcaat agtgcgcgaa
     5041 ctcaccgctc tggagaacga cgagacgctg ctcgtccagt cgggcaagcc cgtcgccgtc
     5101 ttccgcacgc atgagtacgc tccgcgcgtt ttgatcgcca actccaacct cgtcggcgcg
     5161 tgggccacgt gggagcactt ccacgagctt gagcggcggg ggctgatgat gtacgggcag
     5221 atgaccgcag gctcgtggat ttacatcggc acgcagggca tcgtgcaggg cacgttcgag
     5281 acgttcgccg cgatggccga taagaatttc ggcgggacgc tcgcgggccg gctcgtcgtc
     5341 tcgggcggca tgggcggcat gggcggcgcg caacccttag ccgcgacgat gaacggcgcg
     5401 gtcttcctcg gcgtggacgt tgaccgcgag cgcatcgagc gccgcgtccg caccggctac
     5461 tgcgacctcc tcgccgtctc tctcgacgat gcgctccgca tctgcgccga cgccgtcgag
     5521 cagcagcgcg ccgtctccgt tggactcgtc ggcaactgcg ccgaggtgct gcccgagatc
     5581 gttcgacgcg gcgtcaaagt cgatgccgtc accgaccaga cgagcgcgca cgacccctta
     5641 aacggctacg tccccgccgg cctcacgctt gaagaggccg acgagcttcg ccaacgggac
     5701 gcgcgcgtct acgtcgagcg ttcgactgag tcgatggccc gccacgtcga ggcgatgctt
     5761 gaactcaagc gcgcgggggc cgtggccttc gactacggca acaacatccg caagttcgca
     5821 cacgacgccg gctgccgcga cgccttcgag ataccgggct tcgtccccga gtacatccgc
     5881 ccgctcttct gcgagggcaa aggccccttc cgctgggtgg cgctctcggg cgaccccgcc
     5941 gacatccgac gcaccgacga cctcgccctc gaactgtttc ccgaagaccg cacgctcgac
     6001 cgctggctcc ggctcgcccg cgagcgcgtg cagtttcagg gactcccggc gcgtatctgc
     6061 tggctcggct acggcgagcg cgccgagatg ggcgaggcca tcaacgactt agtccgccgc
     6121 ggcgaactcc aagcgcccgt cgccatcggc cgcgaccacc tcgacacagg ctccgtggcc
     6181 tcaccttttc gtgagaccga agcgatgcgc gacggctccg acgccgtcgc cgactggccg
     6241 atactcaacg cgctgctcaa cacggcgagc ggcgcaagct gggtctcttt ccatcacggc
     6301 ggcggcgtcg gcatcggcta ctcgctccac gccggacagg tctcggtcgc cgacggcacg
     6361 ccggaagcct cccgccgtct caaccgcgtg ctcaccaacg accccggcct cggcgtcgcc
     6421 cgacacgtcg atgccggcta cgacgaggcc gaagagaccg cgcgcacgaa gggcgtaaaa
     6481 attccgatgc tcggggcggg cgacgagtag ggccgcgcgt ttgacagcgg ataccagcgc
     6541 ccatatccta ccggcacttc gtttcccgat attccgaaca cagggtttta ctcctcgccg
     6601 atgcgaaaac caaccggagg gtgaaagctg tcttcaaact gtgcctcgtc actcatcctt
     6661 cgctcattca ttaaggagaa aaaatcatgg cagacgagaa agacccgacc gcgagcggcc
     6721 agccttcatc accagaaggc atcatcgtcc ccgacgcggg caacccgtcc gagtctcagg
     6781 agggcatcat cattccccaa gcgggaggcc cgtccgagtc tacggagggc gtcatcgtta
     6841 accaggcgga gcagccgggc gaggcgagcg ccgcgggcgc ggccgccggg gccggtggcg
     6901 cgaccgggac agcttcgagc gaagagggcg acgagcctcc gcagaaggac ggcgtcatca
     6961 tggagttctg acgcctcacc gcgaggcgac gcaaggccag atgccccgtc gcgtctacgg
     7021 acgccccgcg ctgcaagccg cgccgcgcgg ggcgtcggtt tttaaacgcc tcggtcttcc
     7081 ccgccgtgtt tcaacggcga gcgtcgagtg ggtgcggcgc gcagccttcg ggataaattt
     7141 tcgcgggcgc gcgcacgcgg cccgtcagtt tccgcatcag attgccagac gcacctcgcc
     7201 gacgcgttca ccaaacatcc tacccgtgga ggtcagaaga tgaagcaccg ctccagattg
     7261 atcgcgctcg tcgcgctgct gttcgcggcg gcgattcccg cctcggccaa tatcgcgccg
     7321 cccaacgtgc cgacgaagcc gccccggcaa acaaccggag cgcccgtcag gatgcagctc
     7381 cggcccgacg cggacgcgaa ggaagccaag ctgatcatcc cgcgcaaagt cctccaacag
     7441 ctcaaggccg aactcgacgg cggcgcagac ccgcagaccg ccgccgccgc tgtcgcgtcg
     7501 agtgggtcta atctctcctc gacgcagacg gccgtggcgg gcgtcttcct gtcgttgtcg
     7561 ctcgtcctcg gcggcgtgtg gctcgcgggt tcgcgcgcgc cggggaggcg cgtctcgcgc
     7621 gtcgtgtcgg cgctcgcctt cgcggcgctg ggcggagcgg cggcgagcgg cgtggccgtt
     7681 tacgccaacg ccggcccccc gcccgtggcg cgctcgctca cgtcgaagat tctggctccc
     7741 gatctgtctt actggggcgc gagcggcgtg gtgaagatcg agatcgcgaa cgatgagtct
     7801 ctgattatgc tcgtcctgcc gaaggcgaag gacgcggcga cggccgggga gtgaagagtg
     7861 atgcgcgcac ttgccgccca agcaaacgca accgtaacgc tcgtcggcca cgaggcgctc
     7921 gcgccgcgtt tcggcgtgcc gtttttgggc ttctgcctgc tcgcggcgct cttggtcagt
     7981 tggtggccgc tgcaactctc catcgccacc gtcttcctct tcgccggccc gcacaactgg
     8041 atggagtttc gctacttcct ctcgcgcatg cccgcgcgct ggggtcgctc gcgctccttc
     8101 ttcgccgtgg gcctcggcgg cacgctcctg ctcacggccg cctacaccgt gctcttctgg
     8161 ctggggcaaa cgtggtactt gagcgaggcg gcgtggacgg ccggcgtctc cctgtggggc
     8221 acggcgctgc tcgtgtgggc ctgcgcgctc gtgtggctgc gcggacggcg gcgcgcggcg
     8281 cgcggagatt atttgccggt gtacgccgcc ggctcggcgc tcgccgcgct cgcgtgggtc
     8341 gccccgcagt ggttcttcct cgcgctcgtc tacgcgcacc cgctcgtcgc gctctggttt
     8401 ctcgaccggc aacttaaacg cacgcgcccc cattggagga agacttatca cctgtgcctc
     8461 gcggcgctgc cgctgctcct attgctgatg tggagcagac tagccggagc gcccgatctg
     8521 gccgagacgg acgcgctctc gtggcgcatc acgcgccacg cgggcgcaag tatcctgccg
     8581 ggcgtctcca cccacctgct cgtcgcgacg cacgtctttc tggagacgat tcattacggc
     8641 gcgtggctcg tgttgattcc gctggcgggc ctacagcgcg cgggcctctt cgagacgcgc
     8701 cgcatcccgc tcgccgtcca ccgccgaggg ggctggccgc gggccgtcaa agcgtcgctc
     8761 ctcttcggcg cgttcgtcgt cgtcgcgctg tgggccttct tctgggccga ctacacgacg
     8821 acgcgcgatt tgtacttcac tctcgcgatg gggcacgtgc tcgccgaggt gccgtttctg
     8881 ctgcgactac tctgacacca gtaccgcgag cggtagcgag cgggtctcgc gtccgcgagg
     8941 cgggcctctc atcggaggcg tacccgcgag gctaccgctc gcggtactgt ttggagcttc
     9001 aagtcgccat ttacgacctg atgattttcg acatcgcacc cccgcccgtc gtcacctccc
     9061 ggcccgacga gttgtggtat ttccttccgt tcggctacct tctcacggtc gcggtcgaaa
     9121 cgcccgtcct cttcttcggt ctctcgaaga ggctcacgcg gtggcagcga ctcttcgccg
     9181 gattgtggct gacggcctgc acctacccgg tcgtcgtcct cgtactgccg gccctcttcg
     9241 ccacgtcgtc gcgcggcctc tacctgctcg cggccgagac gttcgcgccc gccgccgagt
     9301 gcgtcatctt ctggctcgcc atcggacgcc gcctcgacgt gagcgcccgc gcgaagctgc
     9361 tcaacttcgc gacgattgtc gcggctaaca tcctgtcctt cgccgccggc gaggtgctgg
     9421 gcgcgacccg ctggttcgga ctcttctgag cgacggcgcg acgcgcactg gcttggatca
     9481 gacaacaggc gcaaccgctc ctccatattt tcaaggaact gcaaactaaa atctcgtgga
     9541 cgaagagccg acgcacgtcg agacgcacct gacgcccgac ccggtggccg ccgccgagcc
     9601 gagggcagtg ggcgaagggg gccgtgtgcg gagggcggcg cgggcgctcg ccatcgacat
     9661 cacgccgctc aaggtgtcgc gcgactttcg gttgttgttc ttcgggcaga cggtgacgtt
     9721 cttcgggtcg atgatgacgt tcgtcgtgct gccgtggcag atgtaccgtt tgacggggtc
     9781 gtcgctggcg gtcgggatgc tgggcgtggc ggagttcgtg cccatcctcc tgatggcgtt
     9841 cgtcgggggc gcgctcgccg actacttcga ccgccggcgc atggtgctgc tcacggagct
     9901 ggcactcgcc gccggcaccg ccgtcctcat cctcaactcg ctgctgccgc agccacgcgt
     9961 ctgggtgctc tacgtttgcg cttcgctgtt cgccgcgctc aacggcttgc agcggccgtc
    10021 gctcgactcg ctcatcccgc ggctcgtgcc ggcggaactt ctgacctcgg ccgcggcgct
    10081 caggtcggtg gggctgacgg ccgggatgat cggcgggccg gctttgggcg gcgtgctcgt
    10141 cgccgcgttc ggccccgtcg tcgcttattc gattgacttc gcgaccttcg ccgcctcgct
    10201 cgcggcgctc gccatgttgc gcgccgtgcc gccgccgccg ggcgctgacc ggccgagcgt
    10261 gcgtaccgtc gtcgagggac tgcgctatgc gcgcagccgt cccgagttga tgggcaccta
    10321 cctcgtggac atcaacgcga tgttcttcgg gatgccgatg gcgctctttc cggccatcgg
    10381 cgaaagcctc ggcggcccga aggtggtggg gctgctctac gccgcgcccg ccgccggcgc
    10441 gctcctcgcc acgctcacct cggggtggtc gaagcacgtc aaccgccacg gcctcgccgt
    10501 ctgtctggcc gccgccgtgt gggggttggc catcgtcggc ttcggctttg cgtcgtcgtt
    10561 atggctggcc gttttgtgtc tcgcggtggc gggcgcggcc gacatggtgt cagggctgtt
    10621 tcgcatgacc atctggaatc agaccatccc cgaccacctg cgcggccggc tcgcgggcgt
    10681 tgagatggtc agctacacta cggggccgct gctgggcaac gccgaggccg gcatcgccgc
    10741 ccgtctgttc ggcgtccgcg cgtcggtcgt ctcgggcggt gtgctgtgcg tcgtcgggac
    10801 gggcttgctc gcgctgctgc tgcccgcgtt cctgcgctac gacgggcgcg aggggctgaa
    10861 gcgcaaggcc gcagaagaag aggcgcgcgc cgccttgctg ggcgaggcgt cagaataatc
    10921 agacagaatt gacaggacga acaggatgaa gaaaaactga tttcagattt caattcttct
    10981 tatcctgcct atcggcgtct ggcagtgaag acgggaggag agattttgag acgcacacaa
    11041 ttacgccgcg cgtggcttgc gcctttgctg ctcgtcgtcg ccttcgccgc gacggcgacg
    11101 cacgcgcagg ccgtcgcgcc cgccgccggg acgagcgttg agacaatcaa cttcgagagc
    11161 aagctggtgg gccgccggct gccctacaaa gtcgtcctcc cgcccggcta caacgcggcc
    11221 gcatccacgc cgccgacgct ctacccgacg ctctacctgc tccacgggtt gacgggccat
    11281 cacaccgact ggctcgcgcg caccaagctc tccgactacg ccgcgcagta ccgtctcatc
    11341 atcatcacgc ccgagggcaa cgacggc
//
LOCUS       DASVES010000237        20744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_339598, whole genome shotgun sequence.
ACCESSION   DASVES010000237 DASVES010000000
VERSION     DASVES010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20744
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339598"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF240_09680"
     CDS             <1..275
                     /locus_tag="VF240_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175515.1"
                     /translation="LGEILEKVAQTIRERFKIMEDFKTMTTSARGSAWILSALPFGVV
                     FLLTMLNPGYMSSLLHDARGHYLIAIAITMQLIGMVLINRILKIKI"
     gene            318..1223
                     /locus_tag="VF240_09685"
     CDS             318..1223
                     /locus_tag="VF240_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7175516.1"
                     /translation="MLLLIIIATFGCIALGVMSVYLLTDRQTSVVNARLESMDPTITL
                     MEDSPLTVMAERVADPINRIIPISASEALKLQKQMLQAGYRSPDAATVFRSVQLILMA
                     VLPGIVFTLCYALGRPLNNFFIWGFVALALGFYLPRLYLRRRLVGRQQRITWGLADAL
                     DLMVICVEAGLGLNAALKRVGEELKDVHPEIHHEFELVNLEINVGRSRDEALHNLAER
                     TGVDDIRSFVALMIQADKFGSSIGRAVRIFSDSLRTKRRQRAEQAAQKAALKLLFPLT
                     MFLFPTIIIAVLGPSILNLIDMFGK"
     gene            1250..2233
                     /locus_tag="VF240_09690"
     CDS             1250..2233
                     /locus_tag="VF240_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175517.1"
                     /translation="MRKKITLAVVAAAALTFGGAHLTSAVLASGSNPKSAATEAQKKN
                     GNGFARALSSPFRALARIFSGGKDAKKPAAAPRRMTEKDAASFESATMTRVSNTELGE
                     ATTAAAAASTAGAAYSAPDKVAQARQMLEQERVSEAIGLLSSAALVAPDMAAAHHLLG
                     YAYGRKGLRQLSYQHYERALQLAPDDAQLLNDYGHTLYLRGDYKAAKERLKKATKLAP
                     RDERIWNNLALAQGKLEDYDDAFKSFRQAGGEFKARMNVANMLERTGRDKDALKHYEA
                     ARKLNPTSRSMLQHLADVYQRLGRTDEAEAARQALSAPPQTKSKSEAGGGL"
     gene            2282..3283
                     /locus_tag="VF240_09695"
     CDS             2282..3283
                     /locus_tag="VF240_09695"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7175518.1"
                     /translation="MGPHCTSRHPLRGGTRSFVPRLALVCFALLLPLSSAPALAADRL
                     HLKDGAVIEVDEAWEDAEGVWFRRAGVTQFIGRERVRRIERVSGAEGAAGVKPAPAVE
                     AVKGSDVTAPPPAVKTVWLYLVGGAKMEVDEANESAEGVWFRRANLSSFIEKSRVERV
                     EYAPLASAGETSGGERGRAYGWTTGSAGLDELIRQNGARYGVDPYLIFCVIEQESHFN
                     PRAVSPVGARGLMQLMPATARRFGVRSVHDPAQNVSGGTRYLKELLGMFGGRLDLVLA
                     GYNAGEGTVMKYGRRVPPYRETQNYVKRIGARYGRTSAVAPAKESGGVSSPASAVSQ"
     gene            3296..4087
                     /locus_tag="VF240_09700"
     CDS             3296..4087
                     /locus_tag="VF240_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175519.1"
                     /translation="MSSRRFIYAVLPLLILLSCVRAGAQRRPEGGQVLTNASVIKLVR
                     GGFSDKAVIAIINTRPTRFDLSTERLIDLKKSSVSERVILAMIGREAGEFASAEDDWD
                     MEHDPFFKDAARPPDDKGSSRQSGQGAASPGETNIFGSSGGTRGRTRSRGNNGSVADE
                     TETVGSATVRILRPPAEGGAGGAPKLERTPTLTNESVVRLVEAGFSDGTIIRRIEQSP
                     VEFDLSDGKLAELRKGRVSEPVIAAMRTAMSGDPDPAANKFGGAP"
     gene            4084..5202
                     /locus_tag="VF240_09705"
     CDS             4084..5202
                     /locus_tag="VF240_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7175520.1"
                     /translation="MSGAARSGARRLHIFLVALLLISSAAFSAPMAAPAGRPQRHVRP
                     REVGRPSTQPAPTAGAQAGQGVGDDEVVSVDTLEVLLPVTVRDAQGRLVDALAREDFR
                     VFEDGREQPLRDMVLRQAAADVALMVDASSSSAANLDDFRSAVAQFASKLAPEDRVCL
                     IKFDDRVELLLDWTSSRAQLRRALDRLAGGMFTRFHDALYLAAKEQFRSPQRRHVAVV
                     LTDGIDNGRGATTLEGALRQMLIAQAAVYVISNTEIQRAKKRAELDSILSGPDASVRF
                     SSLRIEDLKESLRVLDASEQSLARLTAATGGRLFVPESFAALEGVYAEVADELSRQYA
                     LYYSPLNKTRDGGFRRVRVGMSKPGLQVTARVGYYAPQ"
     gene            5272..7020
                     /locus_tag="VF240_09710"
     CDS             5272..7020
                     /locus_tag="VF240_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7175521.1"
                     /translation="MSTFADRIDARRGVEIGEGSRAADTPSTPTADALESVSPRMVCV
                     MRFVLALSALIITLLDPSEPNRLVTLTCVALLLYTMYSGILYLVVARTGENPSSPAST
                     WIDVGSYVVFIAASSGAGSIFFFFFFFAIMVASFRNGLKSGLRVTLVSAALFTVVGYA
                     ASAQNEFEMNRFLMRTTCLLVLGYMIAVWGDSEHKLKRRLELLRSIGRSANPRFGIDQ
                     TMCVFMEGVRKFYQADTCLLVTQNPETHEYFLRRADRLKSDRAVGNEMVSGEIGCQLA
                     WLLGEEAVAYEPRRSSGRRGRVLVAGRAPADADDPRLLPAALSLRHLVGDEAFLSAPL
                     RRRDGTAARLYVTSRERPFDGGDLQFLCQVVEHATPHIENHELLDQLASMAANQERRR
                     ISSDLHDSTIQPYIGLKMGLEAVRRKAAPDNPLNGDLSELLMRIEVELADLRRYVRGL
                     KREDHEAAEPVLTAAVRQYAEQFARLSGLDVSCEVQSGLGVSDRLAAEAFQIVREGLS
                     NVRRHTAARRAAVRLSSAGGRLVIEVENEAPGASFRAVPFTPRSIKGRAELLGGMVRV
                     GEGERGVTVVRVEIPR"
     gene            7042..7713
                     /locus_tag="VF240_09715"
     CDS             7042..7713
                     /locus_tag="VF240_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7175522.1"
                     /translation="MTEQRTIRVMIVEDHGVVLWGLKSLVESADPAMCVVGSARNRHE
                     AFKTARAMRPDVILLDVGLGEDNGLDFLPHLMEASGGRVLVLTGVRDEEVHRHAIHRG
                     ASGVVCKDEPADVVLKAIRKVYEGEVWFDRKTAFEVLGELMRGAGSPSADGTAPRVAS
                     LTPKEREIITIIATEESSTNKQVATRLRISENTLRNHLSSIYDKLGVGNRLELLKFAL
                     NHGLT"
     gene            8008..8304
                     /locus_tag="VF240_09720"
     CDS             8008..8304
                     /locus_tag="VF240_09720"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175523.1"
                     /translation="MTERIRTPIPVRIRMVGGGHDADVTSVVDNLGAGGFYVRVPRRF
                     EPGARLSALFRFTADSVETKYVARLAVRGCVLRVEELPGSVYGVAVSICQYKFV"
     gene            complement(8359..8772)
                     /locus_tag="VF240_09725"
     CDS             complement(8359..8772)
                     /locus_tag="VF240_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175524.1"
                     /translation="MALLLAGLPSTLSKGRAPEGGGFDDPTGASSGAGRSANRFVPSR
                     ILLKFRASTSDEHPRRLLAAYGARTGFPGIDVHVVELPATAIEEHFVQTIQAFPEVEF
                     AELDRILQPAVMNPNDPRYGSEWLDPDTCLIISRL"
     gene            9816..10478
                     /locus_tag="VF240_09730"
     CDS             9816..10478
                     /locus_tag="VF240_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7175525.1"
                     /translation="MVNILIVDDHAVLRAGIRLLIETDKRFAVCGEAENCRDALDLAQ
                     STQPDLILLDIDLDGENSLECVPSLLKVSPASRVLVLTGHSDEDVHAQALRAGAIGLV
                     LKKEAASVLLKAIDKVDQGEVWFDRATMGSVLSEMSRGGGRASEDIEGAKIATLTDRE
                     REVVTLIGEGLKNKHIADRLCISETTVRHHLTSIFDKLGVTDRLVLVIYAYRHNLVKP
                     PK"
     gene            10578..12245
                     /locus_tag="VF240_09735"
     CDS             10578..12245
                     /locus_tag="VF240_09735"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7175526.1"
                     /translation="MTKPAEPLDARMVAFIRTVLAFCTFIFTYIDPTAPPTLIPVVYT
                     ALALYCLYSVAIYRAAARGAAFVSSGLVHGLDIVWYLLLVALSGGSNSAYFLLFFFAV
                     LVASFSSGVSSGLRAAAISAALFTLVGYSAIAPNEFQLSRFLLRPVYLLVLGYMIAYW
                     GGRELRLRRQLELLRDVTVTLNPRFGTHHLIGHVVKVLRDFYKARVCLLLLVEEGGDS
                     CLLFRAEAGVGGGAAPAERVPAEAVRDLLPYGGSSVLVCSGARRRLFRRGGCDSFDLE
                     TKEQAGAPPDVFDSVSRALAADNFLSVPVTYQRGVTGRLYVADSARSLGRGDADFIAQ
                     VFEHTRPFIENVRLVDTLASDAAQDERQKIALDIHDGVIQPYIGLQLGLEALRRQADG
                     SNEAVSKGIERLIALTESGIEDLRLYVGGLKAGGERKGSLLPALRRFGVKFSEATGVG
                     VEVESETPDLFLNDRLAGEVFQMTTEALSNIRRHSEAKAATVRIGCDDRRLSLRVTND
                     GPPGDTNTTAVAFVPRSITERASSLGGTVFVRRPPEGGAVVEIDIPL"
     gene            12269..12952
                     /locus_tag="VF240_09740"
     CDS             12269..12952
                     /locus_tag="VF240_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7175527.1"
                     /translation="MSARQDSSEVRIVLVDDHQLFRDGLRMLIESRPGFLVVGEAGDA
                     AGGLEAVAGERPEVVLLDLDLGGEDGLELLPKLLDASGETNVIVLTGIRDPEVHLRAA
                     RLGAKGLVLKDKARGALLKAIERVCAGEVWFDRTLLGTLFSGGRGAGEADMADAAKIE
                     RLTESERGIIAVFGEGMTTTKEIAGRLSIESSTVEKHLTAIYGKLGVQNRLSLVIFAY
                     NSGLLKPSK"
     gene            13173..14645
                     /locus_tag="VF240_09745"
     CDS             13173..14645
                     /locus_tag="VF240_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175528.1"
                     /translation="MSIDNTLYRGRAAALAIFFLLFISPAALFAQNREMPLTASKEAL
                     ALFVQGRDKIENLEDPGTLFEQAIQKDPNFAMAYLFAGRTNQEFRKNLEKAVGLADKV
                     SPGEKEWILAAKEQSEGNPTGRKEHLEQLLKLHPADKRAHSQIAFYYRSIGDDAPSLR
                     HFSEAVKLDKNYAPAYNNIGYANLRMGRYADAERAFKTYIKLIPKNPNPYDSYAELLM
                     KTGKYDESIKQYNRALSVDPTFFNSYRGMGNNYAYKGDYAKARESYKLMFDKAPDDAG
                     RDLALVSEMNSYVAEGKTGEALAVNERRRGMAEKAGDIQTLIGIHTAAGFILVEAGKL
                     DDAAKHFEKADQLREDPSLPAAGRENRRLGSMQNRTRLSIARQDFGPARTQLEEIRQH
                     LSSRKNPNQERGYNETAGFLELGQKNYTKALEYFAKADPNDPYVWYYQAVAYEGAGDK
                     KSAAALYRKVVNWNQLDDTGHAVVRSRALARGVELSKLSK"
     gene            14995..15612
                     /locus_tag="VF240_09750"
     CDS             14995..15612
                     /locus_tag="VF240_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175529.1"
                     /translation="MRITYHLLAAALFITAAAAAPPCFGRGAATTTDDAAAEQSARPA
                     RIFLILTSGTKTEVEEVAEKSEGFWFRRGNVWTLIDRSKVARVERELPAEKVAAAGAA
                     PKAAAARWSLSDAARVESFFVKRFGRALPVTAFGQSGLHNRWGYDHRDSLDVGLHPDS
                     LEGRELMAFLSGEGLPFMGFRSAIPGVASAPHIHIGLPSRRLTSR"
     gene            16044..16469
                     /locus_tag="VF240_09755"
     CDS             16044..16469
                     /locus_tag="VF240_09755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7175530.1"
                     /translation="MAERVALTPRFRAQPREWVERPDRLYERFHVRVRGTDAAGKKFK
                     VTTVLENISEGGLYVLLDRRVEGGAPLSFFVSFSTLPQGEAKNAPRLCARGHVLRAEA
                     QPDGLCGVAVSFTRHRFVQGHNVNGGAWRAANHKGTGTG"
     gene            16613..>20744
                     /locus_tag="VF240_09760"
     CDS             16613..>20744
                     /locus_tag="VF240_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX7175531.1"
                     /translation="MIVTTLATAGFSATSSAGSLHKIIFDTFLSPTAPSVTKGIELKA
                     SSAQGALAPQQGTNAATAMNSSAGAARQGHTATLLGGGQVLVVGGDAGGTAEVFDAAT
                     GTHAVTGGLSLARFDHTATKLADGRVIVIGGRSGGTAEATTEIYNTTTGSFEAGPSMS
                     VARAGHSATLLSDGKILVAGGDAAGSAEVYDPAANSFSAGGAAMGVARSGHSAALMND
                     GRVIVVGGRDAGGNELESAEIYNGSGFSATGNAMAHHRVRAHLRVLPDGKVQVIGGND
                     DDSMEVYDPAVDVFGAHGHLIPVTDPDAALLEDDILGAQTRAAFFHHGQADAVRDREG
                     HTITEIPQLGKAVVLGGVNTEGAALNSASILNSSAATVTTDKLDYAPGQTATISGTGW
                     QPNEVVNIILHEDPHTHTERRLAPQANAEGDFSANYLVDSHDFQVTFIVGAKGASSSR
                     TAQTTFTDGNLNIFTSGPSYTLVSDVYNSANCTGSVPTNTSQTVTLASNAQFSINANT
                     KSYKLTVQGPPAGFTNWTTSSLGTGVSVFSNVGNVLCLNTTSPSGSGNATANFSTAAT
                     ALTVGPASGAYGGTANLSATLKTPNASGAGVSGKTISFTLNGNSVGSAITNASGVATL
                     SNVSLAGINAGSYPAGVAASFAGDASFLASSGSNSLTVSKAATTTTVTCGVGPFTFTG
                     AAQTPCTATVTGAGGLNQSLTVNYTNNTNAGTATASASYAESANHLGSSDSENFTIDK
                     ASSTTVVTCPTNVTYTGAAQTPCSAVVTGVGGLNQSLTVSYSNNTNAGTASASATFAG
                     DTNHTGSNDSKNFTIDPAASTTVVTCPTNVTYTGAAQTPCTATVTGAGGLNQSLTVNY
                     ANNTDAGTATASASFAGDANHLGSSDSENFTIDKATTTTTVTCPTSVTYDGSALEPCT
                     ATVTGPGALNQSLTVSYSNNTSAGTAIASASYAETANYFGSSDSKNFTIDKAASTTVV
                     TCPASATFTGAAIEPCSATVTGAGGLNQSLTVSYTNNVNAGAASASATYAESANHLGS
                     SDSKNFTIEKAATTTVVTCPTNVTYTGAAIEPCTATVTGPALSESLTVNYTNNTNAGA
                     ATASAAYAESANYLGSSDSENFTIDKAATVTTVSCPTNVTYTGAALEPCAATVTGAGG
                     LNEALTVNYTNNTNAGAATASATYAESANYLGSSDSKNFTIDKAATTTVVTCPASATF
                     TGSAIEPCSAAVTGAGGLNQPLTVSYTDNVNAGTATASASYAGDANYLGSSDSKNFTI
                     ERAATTTTVTCPTNITYTGAAIEPCTATVTGPGGLNESLTVGYTDNVNAGAATASATY
                     AETANYLGSSDSENFTIDKAATTTTVTCPTSVTYDGSAQTPCSASVTGAGGLNQSLT"
BASE COUNT         3831 a         6890 c         6610 g         3413 t
ORIGIN      
        1 atctcggcga gattctagag aaggtcgccc agaccatccg cgagcgcttc aaaatcatgg
       61 aagacttcaa aacaatgacg acctcagccc gcggctcggc gtggattcta tctgccctgc
      121 cgttcggcgt cgtgtttcta ttgacgatgc tgaatccggg ttacatgagc tcgctgctcc
      181 acgatgcgcg cggacactac ctgattgcca tcgccatcac catgcagctc atcggcatgg
      241 tgctgattaa caggattttg aagatcaaga tttaacaatg ggggccaagg ttcgccgcct
      301 gtaaggagta ggaaagcatg ttgcttctca tcatcatcgc cacgttcggt tgcatcgcgc
      361 tcggcgtgat gagcgtctac ctgctgaccg accggcagac gagcgtcgtc aacgcccgtc
      421 tggagagcat ggacccgacg atcacgttga tggaggacag ccctctgacg gtgatggccg
      481 agcgcgtcgc cgacccgatc aatcgcatca tccccatctc ggcctccgag gcgctcaagc
      541 tgcagaagca gatgctccag gccggctacc gctccccgga cgcggcgacc gtcttccgct
      601 ccgtccagtt gatcctgatg gccgtcctgc cgggcatcgt cttcaccctc tgttatgcgc
      661 ttggccgccc gctgaataac tttttcatct ggggctttgt cgccctagcg ctcggcttct
      721 atctcccccg gctctacctg cgccgcaggc tcgtggggcg ccagcagcgg atcacctggg
      781 gattggccga cgcgctcgac ctcatggtca tctgcgtcga ggcgggcctc ggactcaacg
      841 ccgcgctgaa gcgcgtcggc gaagagttga aggacgtaca ccccgaaatc catcacgagt
      901 tcgagctggt gaacctcgag atcaacgtcg ggcgaagccg cgacgaggct cttcacaacc
      961 tcgccgagcg gacgggcgtt gatgacattc gcagcttcgt ggcgctgatg attcaagccg
     1021 acaaattcgg ttcgtccatc gggcgcgccg tgcgcatctt ctccgattcc ctgcgcacga
     1081 agcggcgtca gcgtgcggag caggctgcgc agaaggccgc gctgaagctc ctgttccccc
     1141 tgaccatgtt cctgttcccc acgatcatca tcgcggttct ggggccatcc atcctgaacc
     1201 tgattgatat gtttgggaag taatttaaga gcgctaagga gccaaactga tgcgaaagaa
     1261 aatcaccctg gcggtcgtgg ccgcggcggc cctcacgttc ggcggcgcac acctgacgag
     1321 cgcggtgctc gccagcggct ctaacccaaa gtcggcggcg accgaagccc agaaaaagaa
     1381 cggcaacgga ttcgcccgcg ctctcagctc ccccttccgc gcgctcgcca gaatcttcag
     1441 cggcggcaag gacgcgaaga agccggccgc cgcgccgcgg cgcatgacgg agaaggacgc
     1501 cgcgtctttc gagagcgcca ccatgacgcg cgtcagcaac accgaactcg gtgaggccac
     1561 gaccgccgcc gctgcggcca gcacggcggg tgccgcgtat tccgcgcccg acaaggtcgc
     1621 gcaggcgcgc cagatgctcg agcaggagcg cgtgagcgag gccatcgggc tgctgtcgag
     1681 cgccgccctg gtcgcccccg acatggccgc ggcgcaccac ctcctcggct atgcctacgg
     1741 ccgcaagggc ctccgacagc tctcctacca gcactacgag cgcgcgctcc agctcgcgcc
     1801 cgacgacgcg cagctcctga acgactacgg ccacacgctc tacctgcgcg gcgactacaa
     1861 ggccgcgaag gaacgcctga agaaggcgac aaagctcgcg ccccgcgacg agaggatctg
     1921 gaacaacctc gcgctcgcac agggcaagct cgaagactat gacgatgcct tcaagagctt
     1981 ccggcaggcc ggcggggagt tcaaggcgcg catgaacgtc gccaacatgc tcgagcgcac
     2041 gggccgcgac aaagacgcgc tcaagcacta tgaggccgcg cgtaagctca accccacgtc
     2101 gcgcagcatg ctgcaacacc tggcggacgt ttaccagcgg ctcggccgca cggacgaggc
     2161 ggaggccgcg cgccaggccc tgtcggcccc gccccagacg aagtcgaagt cggaggccgg
     2221 cggcggcctg tgagggggcg ccggggcacg tcccggccga tttgtgacgc cgattaattc
     2281 catgggcccg cactgcacaa gcagacatcc gctccgggga ggcacgcgct catttgtccc
     2341 gcggctcgcg ctcgtttgct tcgccctgct gctgccgctc tcctccgccc cggcgctggc
     2401 ggccgaccgg ctgcacctga aagacggcgc cgtcatcgag gttgacgaag cgtgggagga
     2461 cgcggagggc gtctggttcc gccgcgccgg cgtgacgcag ttcatcgggc gggagcgcgt
     2521 gcgccgcatc gagcgggttt cgggcgcgga gggggcggca ggggtgaagc ccgcgccggc
     2581 cgtcgaggcc gtgaagggaa gcgacgtgac cgcgcccccg cccgcggtca agaccgtctg
     2641 gttatacctc gtcggtggcg cgaagatgga agttgacgaa gcgaatgagt ccgccgaggg
     2701 cgtctggttc agacgcgcta acctttcgag cttcatagag aagtcccgcg tggagcgcgt
     2761 cgaatacgcc ccgctcgcct ccgcggggga gacgagcggc ggcgagcgcg ggagggccta
     2821 cggctggacg acgggcagcg cggggctcga cgaattgatc cggcagaacg gcgcgcgcta
     2881 cggcgtcgat ccctatctga ttttctgcgt catcgagcag gagtcgcact tcaacccgcg
     2941 ggcggtcagc cccgtcggcg cgcgcgggct gatgcagctc atgccggcga cggcgcggcg
     3001 tttcggggtg cggagcgttc acgaccccgc gcagaacgtc tcgggcggga cgcgctattt
     3061 gaaagagctt ttagggatgt tcggcgggcg gttggacttg gtgctggcgg ggtataacgc
     3121 gggcgagggg acggtgatga aatacggccg acgcgtgccg ccctaccgcg agacgcagaa
     3181 ctacgtgaag cgcatcggcg cccgttacgg ccgaacgtcg gccgtcgccc ccgcgaagga
     3241 gtcgggaggc gtttcttctc cggcgtccgc cgtatcacag tgacgaggta aagcgatgtc
     3301 gtcgcgaaga tttatttacg ccgtcctgcc tctactgatt ctcctgtcct gcgtccgggc
     3361 cggggcgcag cgccgcccgg aaggtggaca ggttctgacc aacgcctccg tcatcaagct
     3421 cgtgcgcggc ggcttcagcg acaaggccgt catcgccatc atcaacaccc gcccgacgcg
     3481 cttcgacctc tccaccgagc gcctcatcga cctgaagaag agcagcgtca gcgagcgcgt
     3541 catcctcgcc atgatcgggc gcgaggccgg cgaattcgcc tcggcggaag acgactggga
     3601 catggagcat gacccgttct tcaaggacgc ggcccgcccg cccgacgaca aaggctcaag
     3661 ccggcagtcg ggtcagggag ctgccagccc cggcgagacc aacattttcg gctcgtcggg
     3721 cggcacgcgc gggcgcacgc gctcgcgcgg caacaacggc tcggtcgccg acgagacgga
     3781 gaccgtgggc agcgcgaccg tgcgcatcct gcgtccgccc gcggagggcg gcgccggcgg
     3841 cgcccccaaa ctggagcgga cgccgacgtt gacgaacgag tcggtggtgc ggctcgtcga
     3901 ggcgggcttc agcgatggga cgatcatccg gcgcatcgag cagtcgccgg tagagtttga
     3961 cctgtcggac ggcaaattgg ccgagttgcg gaaaggccgg gtgagcgagc cggtcatcgc
     4021 ggcaatgagg acggccatgt ccggcgaccc tgacccggcc gccaacaaat tcgggggcgc
     4081 cccgtgagcg gggcggcccg gtcaggcgcc cggcggctcc acatcttcct cgtcgcgctg
     4141 ctgctcatct cctcggccgc cttcagcgcc ccgatggccg cccccgccgg gcgaccacag
     4201 cggcatgtgc gcccgcgcga ggtgggccgg ccctccacgc agcccgcgcc caccgcgggc
     4261 gctcaggcgg gacagggcgt aggcgacgac gaggtcgtca gcgtcgatac gctggaagtg
     4321 ctgctccccg tcaccgtcag ggacgcgcag gggcgtctgg tggacgcgct cgcgcgcgaa
     4381 gatttccgcg tcttcgagga cgggcgcgag cagcccctgc gcgacatggt gttgaggcag
     4441 gccgccgccg atgtggccct catggttgac gcctcttcgt cgtcggccgc gaacctcgac
     4501 gacttccgct ccgccgtcgc tcagttcgcc tcgaagctcg cgccggaaga tcgcgtttgt
     4561 ctgatcaagt tcgacgaccg cgtcgaactc ctgctcgact ggacttcgag ccgcgcgcaa
     4621 ctgcgtcgcg ccctcgaccg cctcgcgggc ggcatgttca cgcgcttcca cgacgcgttg
     4681 tacctcgccg cgaaggaaca gttcagaagc ccccagcggc gtcacgtcgc cgtcgtcctc
     4741 accgacggga tagacaacgg gcgcggcgcg acgacgctgg aaggggcgct gcgccagatg
     4801 ctcatcgcgc aggcagccgt ctatgtcatc tccaacaccg agattcagcg tgccaaaaaa
     4861 cgcgcggagc tggactccat cctctccggc ccggacgcca gcgtccgctt cagcagcctg
     4921 cggatcgaag acctgaagga atccctgcgc gtgctcgacg cgagcgagca gagcctcgcc
     4981 cgcctgactg cggcgaccgg cggacggctc ttcgtgcccg agagcttcgc cgcccttgaa
     5041 ggcgtctacg cggaagtcgc cgacgagctg agccgccagt acgccctcta ttattcgccg
     5101 ctcaacaaga cgagggacgg cggcttccgg cgcgtcaggg tcgggatgtc gaagccgggg
     5161 ctgcaggtca cggcccgcgt cggctactac gcgccgcagt aggcggactt gaaccaccag
     5221 cactgtttcg tgaaaggcgt cgggcgcccg gtcgtctcgc gagttgattc tatgagcacg
     5281 tttgcggaca ggatagacgc ccgtcgtggg gtggagattg gtgagggttc gcgggccgcg
     5341 gacacgccga gcacccccac cgcggacgcg ctcgaatcgg tcagcccgcg catggtctgc
     5401 gtgatgcgct tcgtgcttgc cctgtcagcg ctgatcatca ccttgctcga cccgtccgag
     5461 ccaaaccgcc tggtcacact cacctgcgtc gcgcttctgc tctacaccat gtatagcggc
     5521 atcctctatc tcgtcgtggc gcgcacgggg gaaaacccct cctccccggc ctcgacgtgg
     5581 atcgatgtcg ggagctacgt ggtcttcatt gcggccagca gcggcgccgg gagcatcttc
     5641 ttcttcttct tcttcttcgc catcatggtc gcttcgttcc gcaacggtct gaaaagcggc
     5701 ttgagggtga cgcttgtctc ggcggcgctg ttcacggtcg ttggctacgc ggcgtccgcg
     5761 cagaacgaat tcgaaatgaa tcgcttcctg atgcgaacca cctgtctgct cgtgctcggt
     5821 tacatgatcg ccgtctgggg cgactcggaa cacaagctca agcgccggct cgagctgctc
     5881 aggagtatcg gcagatcagc taacccgcgc ttcggcatcg accagacgat gtgcgtgttc
     5941 atggagggcg tcaggaagtt ttaccaggcc gacacgtgcc tgctcgtgac gcagaacccg
     6001 gagacgcacg aatacttcct ccgcagggcc gaccgtctca agtccgaccg cgcggtgggc
     6061 aacgagatgg tctctggaga gatcgggtgt cagttggcct ggctcctcgg ggaagaggcg
     6121 gtggcctacg aaccccggcg cagctcgggc cggcgcgggc gtgtgctcgt cgccggccgc
     6181 gcgcccgccg acgccgacga ccccagatta ctgcccgcgg cactgagctt gaggcatttg
     6241 gtcggggacg aggctttcct cagcgccccg ttgcggcgga gggacgggac ggcggcgcgg
     6301 ctgtacgtca cgtcgcgcga gcgcccgttc gatggcggcg atctgcagtt tttgtgtcag
     6361 gtggtggagc acgccacccc ccacatcgag aaccacgaac tgctcgacca actggcctcg
     6421 atggccgcca atcaggagcg ccgacgcatt tcaagcgacc tccacgacag caccatccag
     6481 ccgtacatcg gcctgaagat ggggctggaa gccgtccgcc ggaaggccgc gcccgacaac
     6541 cctctgaatg gcgatctgag cgagctgctc atgaggatcg aggtcgagct agccgacctg
     6601 cgtcgttacg tccgcggctt gaagcgggaa gaccatgagg ccgccgagcc ggtgctgaca
     6661 gccgcggtgc ggcaatacgc ggagcagttc gcccgtctta gcgggttgga cgttagttgc
     6721 gaggtgcagt caggtcttgg ggtgagtgac cgcctcgccg ccgaagcctt ccagatcgtg
     6781 cgcgagggtc tgagcaacgt ccggcggcac accgccgcgc gccgcgccgc cgtccggctc
     6841 tcctcggcgg gcggccggct ggtgatcgag gtcgagaacg aggcccccgg cgcgtccttt
     6901 cgtgccgtgc ccttcacccc gcgctcgatc aaggggcggg cggagttgct cggcggcatg
     6961 gtgcgagtcg gggaaggcga gcggggagtg acggtcgtcc gggtggagat acctcgctaa
     7021 ggaagtttgg aggattctga cgtgacggag caacggacaa tccgggtgat gatcgtcgaa
     7081 gaccacggcg tcgtcctgtg gggtctgaag agccttgtcg agagcgccga cccggcgatg
     7141 tgcgtcgtcg gcagcgcgcg gaaccgccac gaagccttca agacggcgcg ggcgatgcgc
     7201 cccgacgtga tcctcctcga cgtgggtctg ggcgaggata atggtctcga ctttttgcct
     7261 cacctcatgg aagcgtcggg agggcgcgtg ctggttctga ccggcgtccg agatgaggag
     7321 gtgcatcgcc acgcgatcca ccgcggcgcg agcggcgtgg tttgcaagga cgagcccgcg
     7381 gacgtagtgc tcaaagcgat caggaaggtc tacgagggcg aggtctggtt cgaccgcaag
     7441 acggccttcg aggtgctcgg ggagttgatg cgcggcgcgg gttccccgag cgccgacggc
     7501 acggcgcccc gcgtagcctc actaacgccc aaggagcgcg agatcatcac catcatcgcc
     7561 accgaagaga gctccacgaa caagcaggtg gccacgcgcc tgcgcatcag cgagaacacg
     7621 ctgcgcaacc acctctcctc catctacgac aagctgggcg tgggcaatcg tctcgagctg
     7681 ctcaagttcg cactcaacca cggcctcacc tgatccaccg taaaattccc gccggctcgg
     7741 taaacacttc cgacgccctg gcaatctcct ctccacccgg ccccgcgaga atgggaggta
     7801 tgtgcccccc agaatgggac gtccatctca tgcgcgcttc gggtttcaag gctatagtcc
     7861 gcaaatacgg tagggtacgt gtgagacagc catgcctgaa cgaacccggc gcagggccgg
     7921 ggatttacaa ggaggcggat gtcgccagca accataactt ataggtcgcg cgccgcgcac
     7981 aacatgcaag cggtaacgga gcgccgcgtg acagagagaa ttcggacgcc catccccgtc
     8041 aggattcgca tggtgggcgg ggggcatgat gcggacgtga cctcagtcgt cgataatctc
     8101 ggcgccggcg gcttctacgt gcgagtgcca cggaggttcg agccgggcgc acgcctgtcc
     8161 gccctcttca ggttcaccgc cgactcggtc gaaacgaagt acgtggctcg gctcgccgtg
     8221 cgcggctgcg tcctgcgcgt cgaggagctg cccggtagcg tctacggcgt ggcggtcagt
     8281 atctgccagt acaagttcgt ctgaagcggg cgcgccgccg ccagcacgga accgtcgcct
     8341 ggtgagagat tcttcgtttt atagccggga gattatcaaa caggtatccg ggtcgagcca
     8401 ctcgctcccg taccgggggt cgtttggatt catgaccgcc ggctggagga tgcggtctaa
     8461 ctcggcgaac tcgacttcgg gaaaagcctg aatggtctgc acgaagtgct cttcgatggc
     8521 cgtcgctggc aactcgacga cgtgaacgtc gataccgggg aatccggtgc gcgcgccata
     8581 tgcggcgagg agtcggcgcg gatgctcgtc cgatgtgctg gcgcggaact tcaagaggat
     8641 gcggctgggg acgaagcggt tggcggatcg tcccgccccg gacgacgcgc ccgtcgggtc
     8701 gtcaaaccct ccgccctccg gcgcgcgtcc tttgctcaga gtcgaaggca gtccggccag
     8761 cagaagtgcc atcaataaag tttcccgcaa accccctaca acccttactc atctcttcac
     8821 ctcccgcgtt cgcgaagaca gaaacaactt tttcagggcc gtggcccacg gcgcctgagg
     8881 ggttggaagc ttcagggccg gcgcgggccg aatcatcaat ccgcgcttta aacgtcccca
     8941 cggcgagata atgcctttac ggccgccgtc gttctccggc gatgaaaaaa atttctctcc
     9001 tgtcgatttt cgtctctgat taacgcttta aatgatagca aggcagcccc gcgcgacgga
     9061 tgaccacggg tcagaacttt tgatggctgt ctacataaat tgccaggcga cgacgcatca
     9121 agtccacaaa tgtagacaac cctcagccgt tttacccggc ccgctgggtt gccggatttt
     9181 tgcatgtaaa gccttgattt tcaatcatct caaagataat tcaggtgtct ttggtgataa
     9241 gattcggggc gcaccgagga agtgcctggt cggccgacct tcccctcaag gggcatgtga
     9301 gttgctaatt ggaaaattca gggaatcatg agtattataa ttttgtttgt tccttaacca
     9361 atcgcccgga cgcccagata ggactttcga ctcacctctc ttttcagagt ccgggggcac
     9421 ttcaccaagg cgcaacagtt cctcacgacg agagcccccg cggcttgggg gcggcgcagc
     9481 atgtggacgc acatccgttc ctgacgggct gtgcgtatgt tgattgcaaa tggagcgtgg
     9541 aaacattttc cccggaaccg gcaattcaag gtcgtagact tactatattg cgggccaagt
     9601 ttggcgagag agtacgagcc acctcgtgag cgcggctttc ccgcatgcgt tttgatcgtc
     9661 gagtgcggcg accagaggcc gcccactcga cgcagaggat gtttaagaca gtgaaagcaa
     9721 cagcgtccat caatcctcct cgcgtagatg accagaataa ggcaacccgc gcaatccccc
     9781 gaagggagaa gatggctgac cctcagaaaa cccgaatggt taacatcctg atcgtggatg
     9841 accacgcggt tctccgtgca gggattcgac ttctgatcga gaccgacaag cggttcgccg
     9901 tttgcggcga agccgaaaac tgccgcgatg cactcgatct cgcccagagc acacagcccg
     9961 acctcatcct gctcgacatt gacctcgacg gcgagaactc tctggagtgc gtgccgtcgc
    10021 tgctcaaggt ctcgcccgcc tcgcgggtgc tggtgctgac gggacacagc gacgaggacg
    10081 ttcacgcaca ggcgctgagg gccggcgcga ttggcctcgt cctcaagaag gaggccgcca
    10141 gcgtgctcct gaaggccatc gacaaggtgg accagggaga ggtgtggttc gaccgcgcga
    10201 ccatgggaag cgtcctctcg gagatgtcgc gcggcggcgg gcgggcgagc gaagacatcg
    10261 aaggggccaa gatcgcgaca ctgaccgaca gggagcgcga ggtcgtcacc ctcatcggcg
    10321 agggactgaa gaacaagcac atcgctgatc gcctgtgcat cagcgagacg acggtgcggc
    10381 accacctgac ctcgatcttc gacaagctcg gcgtcaccga ccgcctcgta ctcgtgatct
    10441 acgcctaccg gcacaacctc gtgaagccgc cgaagtgacg gcgcgggcga gccgtggccg
    10501 cgactgcacg gaagggagat aagaccgccc cggcgcctcg accgcgcggc ggcgcggcgc
    10561 gctgagccgg ctttagcatg accaagcccg ccgaacctct ggacgccagg atggtggcct
    10621 tcatcaggac cgtcctggcc ttctgcacct tcatcttcac ctacatcgac cctacggccc
    10681 cccccaccct gatcccggtc gtctacacgg cgctggcgct ctactgcctc tacagcgtgg
    10741 ccatctaccg cgcggcggcg cgcggcgcgg ccttcgtgtc ctcgggcctc gtgcacgggc
    10801 tcgacatcgt ctggtatctc ctgctcgtcg cgctcagcgg cggctccaac agcgcctact
    10861 tcctgctctt cttcttcgcc gtcctcgtcg cctctttcag ttcgggcgtc agctccggcc
    10921 tgagggccgc cgccatctct gctgcgctct tcacgctcgt gggctactcg gccatcgcac
    10981 cgaacgagtt tcagctcagc cgcttcctgc tgcgccccgt ttacctgctc gtgctcggct
    11041 acatgatcgc ctactggggc gggcgggaac tgaggctgcg ccgccagctc gaactcctca
    11101 gggacgtgac cgtcacgctg aacccgcggt tcgggaccca ccacctgatc gggcacgtgg
    11161 tcaaagtgct acgggacttt tacaaggcgc gcgtttgcct gctgctgctc gtcgaggaag
    11221 gcggagactc ctgcctgttg ttccgcgccg aggccggcgt cggcggcggg gctgcccccg
    11281 ctgagagagt gccggccgag gccgtccgcg atctgctgcc ctatggcggg agcagcgtgc
    11341 tcgtctgctc gggcgcgcgt cgccgcctgt tcaggcgcgg cggctgcgac tccttcgacc
    11401 tcgagacgaa ggagcaggcg ggcgcgcccc ccgacgtgtt cgactccgtc tcgcgcgccc
    11461 tggccgccga caacttcctg agcgtgccgg taacctacca gcgcggcgtc accggcaggc
    11521 tctacgtcgc ggactcggcg cggtcactcg gcaggggcga cgccgacttc atcgcgcagg
    11581 tcttcgagca cacgcggccc ttcatcgaga acgttcggct cgtggatacg ctcgcgtccg
    11641 atgcggcgca ggacgagcgg cagaagatcg cgctcgacat ccacgacggg gtgatccagc
    11701 cctacatcgg gctccagctc gggctcgaag cgctgaggag gcaggccgac ggcagcaacg
    11761 aagcggtgtc gaaggggatc gagcgcctca tcgcgctcac ggagtccggg atcgaggacc
    11821 tgcggctgta cgtgggtggt ctgaaggcgg ggggcgagcg caaggggagt ctgttgcccg
    11881 cactccggcg cttcggcgtg aagttctccg aggcgaccgg ggtcggcgtg gaggtcgagt
    11941 ccgagacgcc cgacctcttc ctcaacgacc ggctggccgg cgaggtgttc cagatgacga
    12001 ccgaggcgct cagcaacatc cggcggcaca gtgaggccaa ggcggcgacc gtccgcatcg
    12061 gctgcgacga ccggaggctt tccctgcgcg tgacgaacga cggcccgccg ggcgacacca
    12121 acaccaccgc cgtcgccttc gtcccgcgct cgatcaccga gcgggcctcg agcctcggcg
    12181 ggacggtttt cgtcaggcgc ccgcccgagg gcggggcggt cgtcgagata gacatcccgc
    12241 tctgacgcgc gggttcgcta aacagattat gtccgctagg caagacagta gcgaggtgcg
    12301 catcgtgctc gtcgatgacc accagctctt ccgcgatggc ctgcgcatgc tcatcgagag
    12361 ccgcccgggc ttcctcgtgg tgggcgaggc gggcgacgcg gccggggggt tggaggcggt
    12421 ggccggggag cgccccgagg tcgtcctcct cgacctcgac ctcggcggcg aggacggtct
    12481 cgaacttctc ccgaagctcc ttgacgcctc gggcgaaacc aatgtcatcg tcctcacggg
    12541 tatccgcgac ccggaggttc accttcgcgc cgcgcggctc ggggcgaagg ggcttgtcct
    12601 gaaggacaag gcgcgcgggg cgctcctcaa ggcaatcgag agggtctgcg cgggcgaggt
    12661 ctggttcgac cggacgcttc tgggcacgct cttttcgggg ggccgcggcg ccggcgaggc
    12721 cgacatggcg gacgccgcca agatcgagcg cctgacggag tcagagcgcg gcatcatcgc
    12781 cgtcttcggc gaggggatga ccacgacgaa agagatcgcc ggccggctgt cgatagagtc
    12841 ctcgaccgtc gagaaacacc tcacggcgat ctacggcaag ctgggagtgc aaaaccgtct
    12901 cagcctcgtc atcttcgcct acaacagcgg actcctgaag ccctccaagt gacggcgccc
    12961 ccgcaggccc cgactctata cttcggggaa accgttcatc aatcccaaac ggttcttccg
    13021 caatgttgat gctgcggatg ctgccgtagg taaccctgcc ccgctgtgat atagtccgcg
    13081 ccgaatcgag cctgagccca ctcccttccc agcttctctg gaatcaccca gaccatctga
    13141 agcccgtcgc tcaaacacca aggagcttga gaatgtcgat tgacaatacc ctctacaggg
    13201 gccgagccgc ggccctggcg atcttctttt tgctattcat ttcccctgcc gccctcttcg
    13261 cgcaaaaccg ggagatgccg ctcacggcct cgaaggaggc gctcgcgctc ttcgtgcagg
    13321 gccgggataa gattgagaat ctcgaagacc ccggcacgct cttcgagcag gccatccaga
    13381 aagaccccaa cttcgccatg gcctatctgt tcgccggaag gactaatcag gagttcagga
    13441 aaaacctgga gaaggccgtg ggcctcgccg acaaggtctc gccgggtgag aaggagtgga
    13501 ttctcgccgc caaggagcag tccgaaggca atccgaccgg acgtaaagag cacctcgaac
    13561 aactgctgaa acttcacccg gccgacaagc gcgcccactc gcagatagct ttttactacc
    13621 gctccatcgg cgacgacgcg ccgtccctgc ggcacttctc ggaggccgtc aaactggaca
    13681 agaactacgc gcccgcgtac aacaacatcg gctacgccaa cctccgcatg ggcaggtacg
    13741 ccgacgccga gcgggccttc aagacctaca tcaaactcat cccgaagaac cccaaccctt
    13801 acgattcata cgccgagttg ttgatgaaga cgggcaagta cgacgagtcc atcaagcagt
    13861 acaacagggc gctgagcgtt gacccgacat tcttcaactc ctacaggggc atgggcaaca
    13921 actacgccta caagggcgac tatgctaaag cccgtgagtc ctacaagctg atgttcgaca
    13981 aggctcctga cgacgcgggc agagacctgg ccctggtgtc cgagatgaac tcttacgtgg
    14041 cggagggtaa gacgggcgag gcgcttgcgg ttaacgaacg gcggcggggg atggccgaga
    14101 aggcgggcga catccaaacg ctcatcggca ttcacaccgc cgccgggttc atcctcgtcg
    14161 aagcgggcaa attggacgac gccgccaagc atttcgagaa ggccgaccaa ctcagggaag
    14221 acccgtcact gccggccgcg ggccgggaga atcggcgcct cggcagtatg cagaatcgta
    14281 cccgcctctc gattgccagg caggacttcg gccccgccag gactcaatta gaggagatca
    14341 ggcagcacct ctcgtcgcgg aagaacccga atcaggagag gggctacaac gagacggccg
    14401 gattcctgga gctcggtcag aagaattaca ccaaggcgtt agagtacttc gccaaggcgg
    14461 accccaatga cccttacgtc tggtattacc aggcggtcgc ctacgagggg gctggggata
    14521 aaaaaagtgc ggcagcgctc taccggaagg tggtcaactg gaaccaactc gacgacaccg
    14581 gccacgccgt cgtgcgctcg cgcgccctgg ccagaggggt cgagttgtcc aaactgtcca
    14641 agtaatactg accaaacttc ccggcgagta tgaccaccgc acgccctccc tcgggcttcc
    14701 gccttaaggc acatccgggg ccgcaagccc gaccgtgagg gagggcgcac gcaattgcaa
    14761 ctcgctatag tgcgcgaagc cgccgcgccg gtctcgacgg tctgaaggcc cgcggcggcg
    14821 gcggcttcgc cccgcgtcac ccgatctgct tcttctgctc gggcgcccgc gtcagtctct
    14881 gacagggcgc aaggggtggc tcatttaggc cgtctgaaaa ctgtcgcttt gtccgattgc
    14941 cggggcaggt gggctgcccg tacaataccc gttcgccccg acgcctcttg ccccatgcgt
    15001 attacttacc acctactcgc cgccgccctc ttcatcacgg ccgccgccgc tgctccgccc
    15061 tgcttcggcc gcggcgcggc cacgacgacg gatgacgcgg ccgcggaaca gagcgcgcgg
    15121 ccggcccgca tcttcctcat cctcacgagc gggacgaaga cggaggtcga ggaggtggcg
    15181 gagaagtccg agggcttctg gttccggcgc gggaacgtgt ggacgctgat cgaccgctcg
    15241 aaggtggcgc gcgtcgagcg cgagttaccg gcggagaagg tcgcggctgc gggcgccgcc
    15301 ccgaaggccg cggccgcgcg ctggtcgctc tcggatgcgg cgcgggtcga gtcgttcttc
    15361 gtaaagaggt tcggccgggc gctgcccgtc acggccttcg ggcagtcggg tttgcacaat
    15421 cgctggggct acgaccaccg ggactcgttg gacgtgggcc tacacccgga tagccttgag
    15481 gggagggaac tgatggcctt cctctccggc gagggcctcc ccttcatggg cttccgcagc
    15541 gccatccccg gcgtagcctc cgccccgcac atccacatcg gcctcccctc gcgccggctc
    15601 acctcgcgct aaagtccgca gccgcacgct cctcctcatc ctccttcccc agacccttct
    15661 ttaaatctct tacagacacg tccccgactt ttgagtcggt caagcgtccg tgtgtgcgag
    15721 acgggcgcgc gcgtgcgagc gcgtgcgtcc ccgtcgagac cccactgccg gcgcggccgg
    15781 tcgcgccggg tgaaggtcac ggggtagcag gaaactgcta tcggcctctg gctatttgtt
    15841 ggccgggaga agggatacag tagcagtaaa taacctcgaa aaatgggcaa tcactccgat
    15901 gtgctcttca cgaaaagaca ttatcttgtc atcgtcctca aagtgagatt tcggccgtgc
    15961 ggcctcacga aagtccaaaa agtaaattgc ccggggaagc gcaagcgggc gtgcatgaga
    16021 gagaaagcat gacgcccgaa tccatggcgg aacgagtggc gctgacgccc cgattcagag
    16081 cgcagccccg cgagtgggta gagcgtcccg accgtctcta cgaacgtttc cacgtgaggg
    16141 tcagaggcac ggacgccgcg ggtaaaaaat tcaaggtgac gacggttctg gaaaatatca
    16201 gcgagggcgg tttgtacgtg ctcctcgacc gcagggtcga aggcggcgcg ccgctctcgt
    16261 ttttcgtcag cttctcgacg ctccctcagg gggaggccaa gaacgccccg cgcctgtgcg
    16321 cgcgcgggca cgtactacga gccgaggctc agccggacgg cctgtgtgga gtggccgtct
    16381 cattcacgcg ccacaggttc gttcaaggac acaacgtcaa cggaggcgct tggcgcgccg
    16441 ccaaccacaa aggcactggc acaggctgag caccccgaac taagccccca ccagcataag
    16501 agataaaagg attgccccca tgaatagcag caagaattcc cccacgactc gtactcgcca
    16561 cccgtttagc atcgtcttcg ccaaatcgcg gactcggctg gcggcaattt taatgattgt
    16621 gacgacgctg gccaccgcgg gcttctccgc gacctcctcg gcgggctcgc tccacaagat
    16681 catattcgac acgtttttaa gccccacggc tccttccgtg acgaagggca tcgagctgaa
    16741 ggcctcgtcg gcccagggcg cgttggctcc tcagcagggc acgaacgcgg cgacggccat
    16801 gaactcttcg gccggggcgg cgcgtcaggg ccacacggcg acgctgctcg gcggcggcca
    16861 agtcctcgtc gtcggcggcg acgccggcgg caccgcggaa gtgttcgacg cggccacggg
    16921 gacgcacgcc gtgaccggcg gtctgagcct cgcgcgcttc gaccacacgg cgacgaagct
    16981 ggcggacggg cgcgtcatcg tcatcggcgg gcgcagcggc gggacggccg aagccacgac
    17041 cgagatttac aacacgacta ccggctcctt cgaggccggg ccgtccatga gcgtcgcgcg
    17101 tgcaggtcac agcgcgacgc ttttgtctga tgggaagatt ctcgtcgcgg gcggcgacgc
    17161 ggcgggcagc gccgaggtct acgacccggc ggcgaacagc ttcagcgcgg gcggcgcggc
    17221 gatgggtgtt gcgcgctccg gccattcggc ggcgctgatg aacgacggcc gcgtgatcgt
    17281 cgtcggcgga cgtgacgccg gcggcaacga gcttgagtcg gccgaaattt acaacggctc
    17341 ggggtttagc gcgacgggta acgcgatggc ccaccaccgc gtgcgcgcgc atctgcgcgt
    17401 gctgcccgac ggcaaggtgc aggtcatcgg cggcaacgac gacgactcga tggaagtcta
    17461 cgacccggcg gttgacgtgt tcggcgcgca cggccacctg atcccggtca ccgacccgga
    17521 cgccgcgctc ctcgaagacg acatcctcgg cgcgcagacg cgcgcggcct tcttccacca
    17581 cgggcaggcg gacgccgtgc gcgaccgcga gggccacacc atcacggaaa tcccgcagtt
    17641 gggcaaggct gtcgtgctcg gcggcgtgaa caccgagggc gccgccctca actccgcctc
    17701 catcctgaac agctccgccg cgaccgtgac gacggacaag ctcgactacg ctccgggtca
    17761 gacggccaca atttcaggga cgggctggca gccgaacgaa gttgtcaaca tcatccttca
    17821 cgaagacccg cacacacaca cggagaggcg gctcgcgcct caagctaacg ccgaaggtga
    17881 tttctcagcc aactacctcg ttgacagcca cgactttcag gtgacgttca tcgtcggagc
    17941 gaagggcgct tcgtccagca ggactgcaca gacgacgttt actgacggaa atttaaacat
    18001 attcacgagc ggcccgagtt atacactcgt gtccgacgtg tataactcgg cgaactgtac
    18061 gggttcggtg ccaactaata cgtcacaaac cgtcacctta gcttccaatg ctcagttctc
    18121 cattaacgcc aacaccaagt cgtacaagct gaccgtgcaa ggcccgcccg ccggcttcac
    18181 gaactggact acttccagcc taggcactgg tgtttcagtt ttcagcaacg tcggaaacgt
    18241 actttgtctt aacaccacta gccccagcgg gtctggaaat gccaccgcta acttcagcac
    18301 cgcagccacg gcactcactg tcggtcctgc gtctggcgcc tacggcggca cggctaatct
    18361 ctcggccact cttaagactc ccaacgcaag cggcgccggc gtaagcggca agaccatcag
    18421 cttcacgctc aacggcaaca gcgtcggctc agcaatcacc aacgcgagcg gcgtcgccac
    18481 gctttctaac gtcagcttgg caggcatcaa cgcgggcagc taccccgccg gcgttgccgc
    18541 cagcttcgca ggcgatgcga gcttcttagc cagcagcggc agcaactccc tgacggtgag
    18601 caaggctgcg accaccacga cggtaacctg cggcgtcggg ccgttcactt tcaccggcgc
    18661 ggcgcagacg ccctgcacgg ccacggtcac gggtgcgggc ggcctgaatc agtcgttgac
    18721 ggtcaactac accaacaaca cgaacgccgg gacagctaca gccagtgcga gctacgcgga
    18781 gagcgccaac cacctcggca gctctgactc ggagaacttc accattgaca aggcttcgtc
    18841 cacgaccgtc gtcacctgcc cgacgaacgt cacctacacg ggcgcggcgc agacgccctg
    18901 ctcggcggta gtgaccggag tgggcgggct caaccagtcg ctgacggtaa gctactcgaa
    18961 caacacgaac gcgggcaccg caagcgccag cgccaccttc gcgggagata cgaaccacac
    19021 cggcagcaac gactcgaaga acttcacgat tgatccggcc gcctcgacga ccgtcgtgac
    19081 gtgtccgacc aacgtcactt acactggcgc ggcgcagacg ccttgcacgg ccacggtcac
    19141 cggggccggc gggctcaatc agtcgttgac ggtcaactac gcgaacaaca ccgacgccgg
    19201 cacggcgacg gcgagcgctt cattcgccgg cgatgccaac cacctcggca gcagcgacag
    19261 tgagaacttc accatcgaca aggcgacgac gacgacgaca gtgacctgcc cgacgagcgt
    19321 cacctacgac ggctcggcgc ttgagccgtg taccgccacg gtcaccggcc cgggcgcgtt
    19381 gaaccagtcg ttgaccgtga gctactcgaa caacacgagc gcggggacgg ccattgccag
    19441 cgcctcctac gccgagaccg ccaactactt cggcagcagc gactcgaaga acttcacgat
    19501 tgataaagcc gcttcgacga ccgtcgtcac ctgcccggcg agcgcgacct tcaccggcgc
    19561 ggcgattgag ccgtgctcag ccaccgtcac cggcgcgggc ggcctgaacc agtcgttgac
    19621 cgtcagctac accaacaacg tcaacgcggg ggcggcctcg gccagtgcga cctacgcgga
    19681 gtctgccaac cacctcggca gttccgactc caagaacttt acgattgaga aagcggccac
    19741 gaccaccgtc gtcacctgcc cgactaacgt tacctacacc ggcgcggcca tcgagccttg
    19801 cacggccacg gtgacggggc cggccctcag cgagtcgttg acggtcaact acaccaacaa
    19861 cacgaacgcc ggcgccgcga ccgcgagcgc ggcctacgcg gagagcgcca actaccttgg
    19921 tagcagcgac tcggagaact tcaccattga caaggcggcg accgtcacca cggtgtcttg
    19981 cccgacgaac gtcacctaca cgggcgcggc tcttgagcct tgcgcggcga cggtgacggg
    20041 cgcgggcggc ctgaacgagg cgttgacggt caactacacg aacaacacga acgccggcgc
    20101 cgctacggcc agtgcgacgt atgccgagtc ggccaactac ctcggcagtt ccgactcgaa
    20161 gaacttcacc atcgacaagg ccgcaacgac caccgtcgtc acgtgcccgg cgagcgcgac
    20221 cttcaccggc tcggcgattg aaccctgttc ggcggcggtg accggcgccg gcgggttgaa
    20281 ccagccgttg acggtctctt acaccgacaa cgttaacgcc gggacggcga cggccagcgc
    20341 ctcctacgcg ggagacgcca actacctcgg cagctctgac tcgaagaact tcaccatcga
    20401 gagggcggcg acgacaacga ccgtgacgtg cccgacgaac atcacctata cgggcgcggc
    20461 catcgagccg tgtacggcca cggtcaccgg tccgggcggg ttgaacgagt cgttgacagt
    20521 cggctacacc gacaacgtca acgcgggcgc cgcaacggcc agcgccacct atgccgagac
    20581 cgctaactac ctcggcagca gcgacagtga gaacttcacc atcgacaagg cggccacgac
    20641 gacgacggtg acctgcccga cgagcgtcac ctacgacggc tcggcgcaga ctccctgttc
    20701 ggcgagtgtg accggcgcgg gtggtctcaa ccagtccttg acgg
//
LOCUS       DASVES010000238        24827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_45295, whole genome shotgun sequence.
ACCESSION   DASVES010000238 DASVES010000000
VERSION     DASVES010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 24827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24827
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45295"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VF240_09765"
     CDS             <1..684
                     /locus_tag="VF240_09765"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEX7175532.1"
                     /translation="LAFEKGLNFPFYIYNEDGTNRRENITDWALEKFHAQYGDASITK
                     WDIFHYVYALLHHPAYRERYSANLKRELPRVPFAPDFRAFAEAGARLADIHVNYERQP
                     EYPLERIENPREPLNWRVERMKLGKDKTQIVYNDFLTLAGVPAAAFEYRLGNRSALEW
                     VIDQYQVSTDKRSGITNDPNRADDPQYVVRLIAQVVNVSLETVRLVRALPALGLAESR
                     KAGDGSQSL"
     gene            882..2075
                     /locus_tag="VF240_09770"
     CDS             882..2075
                     /locus_tag="VF240_09770"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX7175533.1"
                     /translation="MNNFVPRLLALVKVSLALAAATVLLAGAQVSAQCPGVTTVTAGL
                     RLPLGITQSNQDNLLVSETGFGAPGTGRISIVGPGGSRRTLLDGLPSGINDVGDPSGP
                     AGLSLRGRTLYVAIGVGDVGVAGPIPGTTVPNPNGPSSPLFSSVLAIHFSAHVEKTTA
                     GFTLTAAQQQALAGGQKVTLSNGGGDKITIELVVDFPNYISNPLPFFADNIRLSNPFD
                     LVAVGGQLYVTDGGRNLVWQVDIDSGASSVLANFPPLANPLPFGPPFIDAVPTGIAYS
                     DGQLLVTLFRGFPFPPGASEVRAVDPETGAQAPFITGRSSAIDVLSFRSKGDTDYLVL
                     EFSADMLAGDPGRLLRFETPTSTPTVVAGCLITPTSMTLDEKTDTLYVTELGTGRVVS
                     IPVAP"
     gene            2244..3023
                     /locus_tag="VF240_09775"
     CDS             2244..3023
                     /locus_tag="VF240_09775"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7175534.1"
                     /translation="MNNPFVPTVLRFDSLPSTNTEAARQAAAGAPEGLVVVAREQTAG
                     RGRQQRVWSSPRDAGLYLSVLLRPRLPAVEWPLLTLAAALAVSDALEETCALRTDIKW
                     PNDLLAGGRKLCGILAETVESERGRACVVGIGVNLKASAYPPELRASATSIEEATGAL
                     AGDNSLLAGVDSLIESLVRAVGRRYARLQSEGGARETRREWSARSSYAEGRRVRVRLD
                     GESFEGVTRGLEPDGALRVESEGGAVRTVRAGDVTALRAAL"
     gene            complement(3044..3712)
                     /locus_tag="VF240_09780"
     CDS             complement(3044..3712)
                     /locus_tag="VF240_09780"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7175535.1"
                     /translation="MSLPRTELIYSIEEYLAIERESEERYEYLDGVIYEMAGESPAHG
                     AICINIAGQLYAQLRGTPCQAFSKDTKVRSGPAPKSRRSSKGLYSYPDLVVVCGELKH
                     IDEHRDVLLNPSVIVEVLSPTTEAFDRGEKWLRYQRWLPTLTDYVLVSQIAPHIDHFG
                     RLPDGPWIYTPALGLEAVLRLDSINCTLQLADVYDRVVFPPEEEPEDDDDEPDLLRPT
                     GRHS"
     gene            3801..5093
                     /locus_tag="VF240_09785"
     CDS             3801..5093
                     /locus_tag="VF240_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175536.1"
                     /translation="MTGKRIILSTFGSFGDVHPYIAIALELKRRGHRPVIATTEMYRE
                     KIEPLGLELHRVRPDMPSYDEPERVAAMIEYVVDEKRGPERLLQTMIVPHLRDAYEDL
                     LAATEGADLLLTHPLPFVGPLVAAKTGVPWVSSVLAPGSFLSVYDPPVPPQFPSAHKL
                     MAASRHLSRVVMWLARKKIDALLGPIYEFRRQLGLPRGGNPMMEGQHSGALVLALYSK
                     VLGAPQPDWPANVRVTGFPFYDRRDRAGDPTGLAPELEKFLDAGEPPVVFTLGSSAIW
                     VAKDFYTESIKAARALGVRALLLIGDERNRPADVLPEGVAAFDYAPYGELLPRARAIV
                     HQGGVGTTGQALRAGRPTLIVPHAFDQHDNAERAARLGASRTLRRTRYNADAAARELR
                     ELLGAESYARRAEEVGRVVQSEDGGRAACDAIEEELNH"
     gene            5236..5550
                     /locus_tag="VF240_09790"
     CDS             5236..5550
                     /locus_tag="VF240_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175537.1"
                     /translation="MTTDVLTEEAFARNLNTTFRVPLGDGRKVELELVEVAGYKDRPD
                     EQQGMERFSLFFEGPAEPFLPQASYTMEHERMGEVLIFIVPIASTGGGFRYQAVFNFH
                     RK"
     gene            5620..6573
                     /locus_tag="VF240_09795"
     CDS             5620..6573
                     /locus_tag="VF240_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7175538.1"
                     /translation="MRLIFTASLHLVILLASISPQAQTRPRGTRRPPPRPPTTSVTRP
                     ESVGEDEVLRVTTNLVTVPVSVRGHDGSYVFDLKQGDFRIYEDGVEQEITHFSSVEQP
                     FTVVLLVDTSSSTEPNLGEIKEAANAFFGQLRQKDSVLPVTFDGQVRPLLSKGTSSRT
                     VLRDAVERARTDTGNNGTKLYDAVDYAYQVLRRVSGRKAIILFTDGDDTWSSATMRST
                     LCNTAELDALIYPIHYGSSPSTNYLRALASETSGRYYQADNVEMIKQSFAAVAEELRR
                     QYSIGYYPKATSRHQQERQLRVEVKGPAAGVTTRKSYLYRP"
     gene            complement(6814..7251)
                     /locus_tag="VF240_09800"
     CDS             complement(6814..7251)
                     /locus_tag="VF240_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175539.1"
                     /translation="MLVLATGVSLSVTDLAFGQQTGTGGSDSGQGFPIPPESRLDPLN
                     NFTKATFQANLNTVFRINAGASNAVELTLIEVSNIGPVPDQTVAGRECFVLRFSGPLN
                     QPLPQRTYLLEQDALGSFDLFIVPGKKDKKWLYYHAVINRLNP"
     gene            7787..9319
                     /locus_tag="VF240_09805"
     CDS             7787..9319
                     /locus_tag="VF240_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175540.1"
                     /translation="MVIGFGLAVSLAGPLGGEVRGTIVGLADYFTDGAGKDKDKPPHT
                     PPGSSAGQTASRATGDSPSGPAFRISRASAAGPNRNVKSADVAYNRDDKEYLVVWQGD
                     GLNGANLRGVDEIFGQRVNAATGREIGPNFLISNMSVCGTDCDANKPRIAYNGAAREY
                     LVVWHGSGLIDMPGKISEVYGQRISRGGDEIGSDFRISNTTDLGKVNGNFVRASAQAD
                     VAWNSVNNQYLVIWSGMGQPEDVVKLEIYGQLLTSGGEAVGNDFRISNTTEQGNNFHA
                     STPAVAFNSTNNQYLVVWTGGFKEESRTEVWGRALAAKGGPLGDGDFRISQVSTAAGS
                     NRRAGSPHVVYNGSNNEYFVAFHANGLAGEANEGVNEIFGQRIDAAKLTETGPNDFRV
                     SNMGGAGNGAIRPRVIYNSFDKEYLVIWRGVRANVPPEVFGQRVSLSGTEIDADFQVS
                     NIAAVGKDRSVNAAVVAHGGDGGEYLVVWHGNALPDATGKAINEVFGQRVRIMPARGT
                     RQ"
     gene            9769..10239
                     /locus_tag="VF240_09810"
     CDS             9769..10239
                     /locus_tag="VF240_09810"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX7175541.1"
                     /translation="MSFVFAPKGWALCNGQLLPINQNQALFSLLGTTFGGDGRVNFAL
                     PDLRGRTPIHVGSGHTLGERGGEQAHTLSIAELPTHIHTLQASANDGTTLVPSSNVVL
                     AKRAAEIYHAPTNLVAMNAASVPNVGGSQAHLNMQPFLTLSFCIALQGIFPSPN"
     gene            10280..10753
                     /locus_tag="VF240_09815"
     CDS             10280..10753
                     /locus_tag="VF240_09815"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX7175542.1"
                     /translation="MFAGNFAPAGWMFCEGQLLPISENETLFQLIGTTYGGDGESTFS
                     LPDLRGRIPIHQGNGFILAETGGAEEITLTVNQIPAHTHPLLASADQANTPNPQGNVL
                     SETVTTTPYFNDVPNTNLNPVSITPVGGSQPHTNFQPYLCIDFIISLFGIFPSPT"
     gene            10766..11287
                     /locus_tag="VF240_09820"
     CDS             10766..11287
                     /locus_tag="VF240_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX7175543.1"
                     /translation="MADPFVAEIRIFPFNFAPKGWAWCNGQLLPLSQNTALFSLLGTT
                     YGGDGKSTFALPDLQGRAPMHPGQGPGLSLHDLGETGGSETVSLLISEIPAHTHALRA
                     SSEIGEDRTPAGEAIAQSTGGFLYRTPADGLVPMAFQGLPPAGGDQPHNNLQPYLTFY
                     FNIALQGVFPPRT"
     gene            11850..16871
                     /locus_tag="VF240_09825"
     CDS             11850..16871
                     /locus_tag="VF240_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX7175544.1"
                     /translation="MKSLTAPAPHSNARAYFAILISFFLVALPVTPVMAASAASAAPV
                     GSAKPRPEPIRSDFKSADSDAGGEAVPAEALGAPPVPAALAPVVTASKADSFPAGGDV
                     DNDGKADQGDTIEYTVTINASGENATGVSFSDTIDGSTTLVPGSVTVTPVAINDSYIA
                     TGNVRIQVPAASGVLLSGDDDYLGAPAATLSGFGNALGNANGTAPGGTVTTTNGGTVI
                     LASDGSFSYNPGPGFEGADNFFYTLTNSAGSNVGQVTITVSDMIWFVNDDDPTPGGDG
                     RLTNPFNCLVGAGCFDQVAADGAGDNIFLYSGSYTGGLTLLNNETLIGQGAGDTLANI
                     AGITLAPHSDALPNTGGARPTVSAGSGNITLGTGNLLRGFNIGNSGAGAAISGTNFLA
                     LAVSEMTINGTGQALNLTNGTLNVSLDSITSTGGSKGVSLTTITGGSLTSGSTNVQTP
                     SGVGVEVIGSVANVSFANTTVNGSGGTGVRLDNNTGNVSFADLDITPNSGQRALYATN
                     NAISSGSITATSGTISTTNAVAVEITRASPTTSLSMALTSVSASGGTSGIVLQNTSGS
                     FAVLGTTAGICGGSVTNTTNPAVAATVTAANTADCTGGTIQNMTADGIRLNNARNVSL
                     TRMQIVNGAQSGIFGTAVNGFELISSFISNNGNAADEVGLKFTDVVNPGTVNGLVGTA
                     LNGANPTRIINSTVRQSGEFNVEVVNRTGTLTDLFVDGCQFTDTKLNAGPADADGFNV
                     EMQNTAVATVRLQNSVFTNNFTQGAQLSAIDSANLTATVTSSTFTNNNEGFVCNHSGN
                     SDLVCTVGGDTAAQGNHFEGEPGGAGTDTGALIVASTGSTGTVSASLTARIKHNHVTA
                     ANNQVNHSIIAFMSGVGTPGSVNISNNTVNHQVFGHAILVDTPDNAASPNFGVTANNN
                     AVTNNAPGGHGVLVQARQNSTACVLIQNNTGTVAAGSSVARIRQNSVAGQNSTLNLKQ
                     GVSASNVATQVMIDNNPGVITSVNGAINVVANGACTTPTLAPSTIEITKTESVPSEIG
                     KRGAHATEDATPTSSMGGVTSRPFVSMPSKQAAAPAASAPASPATLAAPAPVALGGGG
                     DEEAETPASGPNSVGDPLGSGGTVSVNIGTLAPGDSVTITFQVVIDNPFTGNPAQVSN
                     QGTVSGSNFANVLTDDPSVGGSADPTVTLIAVPPDVFIRDGRIAEPATGSANMIFTVV
                     LSAPATGALTVNFTTADEPAGPGKAVAGADYTTTSGSVVFAAGENLKTISVPVLSDAS
                     AEPDETFLVNLTGATGSFTAILDNQAVGTITVNAPGTFLISEVRTSGPGGAGDDYVEV
                     YNNSDTPLTVQTTDGSAGYAVVATNNGCSGDPVILGIIPNTTVIPARGHFLFVGPTYS
                     LTAVAAGNLTLTAGINDDANVGLFSTSTLINLSSVAKLDGVGFGLNTADLCDLIREGA
                     TLPTAQGSTQQYAFVREFTLVAVNVPTPTDANDNATDFTVVSTTASTPVSAAITAPTL
                     GAPGPQNMASPKLKKYSQVGVQYLDPTVAPNLAPNRVRKGCATPGVEECLPNRSAQGT
                     MHLRRTFTNNTGGPISQLRFRVYDITGFPVTVAGDADMRLINAPQVTGVTGPSIGTVT
                     VEATTLDGPTQTTFGGGLNSSVTAGIITLGNQLGNGASIHINFMLGVQQTGNYRVFVI
                     VEALP"
     gene            17029..17856
                     /locus_tag="VF240_09830"
     CDS             17029..17856
                     /locus_tag="VF240_09830"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX7175545.1"
                     /translation="MLLVIDVGNTNTSLGVYEGERLAAHWRLTTARERTVDEYGVHAR
                     NLFALAGLDFKAITGVCIASVVPPLNFTLKRMSEDYFHLTPLFIDHGTDAGLEILYEP
                     PSDVGADRIVDAVAALSKYGAPCIVVDFGTATTFDAINGRGQYLGGVITPGITISSDA
                     LFARAARLPRVEIRRPERVIGSSTVGSIQSGLYYGYAGLVDGILRRMIDELRGEGSAE
                     GERVRVVATGGLAPLIATASELIETVDDTLTLDGLRLIYGRVQSSKFKVQSPEADTA"
     gene            17945..18733
                     /locus_tag="VF240_09835"
     CDS             17945..18733
                     /locus_tag="VF240_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175546.1"
                     /translation="MNYFNYYTEIEDTFVRRRGKHLLLSPLDWALIESWRTMGVPLHV
                     ALRGIEKSFDSYEARPRNRSVKSLFYCQEEVEAQYADWLEMQRGAAALPGQTVSADGV
                     VTAAGATAEGEGREVETSARLPFPRAVIAGHLAESRAAVERAAVERRGGAGAAGADEL
                     SEALERVSSRLSELERDFGAAARPDAEQLEAALTDLEGLIDRAVRAGIGADALASARA
                     EAEGQLRPYRARMDVAVYETTLENILAKALRERFRLPRLSLFHL"
     gene            18779..19183
                     /locus_tag="VF240_09840"
     CDS             18779..19183
                     /locus_tag="VF240_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175547.1"
                     /translation="MANMMVRRVGVLSLAKISGIAGAGLGLIIGVIYGLLIMVIGATA
                     LAGRNGPGAGFGIGFGLLVMIMVPVMYSIFAFIIGAIYALIYNVAAGFVGGIELELEP
                     TDTGYSQPPPPPQQQWAPPPYGQASSGPYDAR"
     gene            19184..20434
                     /locus_tag="VF240_09845"
     CDS             19184..20434
                     /locus_tag="VF240_09845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX7175548.1"
                     /translation="MKDRRSRQKRAKPHPQAVSAGAPAVGEEFEVEVERILPGGLGLA
                     HAAGHTLMVALSAPGDRARVCVRQVRGRVGFASIEELLSPGPARTAPPCPYFGRCGGC
                     DFQQLTYEAQLAAKVEIVRDCLRRVARLEPPADIHITPSPDPWRYRSRAQWQYDTASG
                     ALGYFERGTHRVCDVVESPVVIPPLQATLGSLRDAERDGSLSASVAEFHAVAGDEGVG
                     LAPPVEESHAREQTRTIGGERYSFDAECFFQINHALLDALVREGLRDASGHTALDLYC
                     GVGLFTLPLARRFTRVWGVEGNARAARFARRNLADAGLDNARVETAPVGQWLARRISE
                     PFAADFVLLDPPRAGADPQTLASLIALRPTRISYVSCDPATLARDLRALTERGYRLAS
                     VHAFDMFPQTHHVETVVHLSRSEY"
     gene            complement(20588..21916)
                     /locus_tag="VF240_09850"
     CDS             complement(20588..21916)
                     /locus_tag="VF240_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175549.1"
                     /translation="MSNTKCPRCGLVSWPSAEVCERCGVPLVGGGDPEASATESARPD
                     FPVLTLGRDVPTGSSIWKLILVVVFAVALCGAGWAIYKRRADSRRGGSMVEARLKELA
                     PPSLDERTRDTVLSYLAQPSFAGMRVVEQVLGPVRLEMLQLPERELYFSAPAPSQTGE
                     TFEAILARFIVDLKQVVLAEDDGAGRLRLGKYSLVRSTEKHFFFKTPVDNVRFDPETV
                     LKFHFGSAIYTLDMRELSDFLQNKSIFGGRMHARTGQSRAGLPVVFANHGAFVARPEE
                     TSLRRFVGGLTHDIPADGEGAREARVQRVLDFVSREITYDLRAASYNFETLKRPNEVL
                     MTGESDCSNKAILLGSLLEQLGEDYLFVYTPDHITVAVRQGGFPARNGLSLEWEGQTW
                     LIAEGTVPGFRIGVDRVREEARLKQFQHVQRPRERDVIFDIATGRQLSFQ"
     gene            22191..>24827
                     /locus_tag="VF240_09855"
     CDS             22191..>24827
                     /locus_tag="VF240_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEX7175550.1"
                     /translation="MQLLKGRALAILFLSFTLMSAQAAPQGERQLTGFSRDGAEAQRA
                     LEARFDALLRKENLREWMRRMAARPHHVGSPYGKENAEFMAGLFRSWGYEVEVERFDV
                     LFPTPKVRVLEMTAPERFRARLEEPALKEDATSNQRSEQLPTYNAYSVDGDVTGPLVY
                     VNYGVPRDYEELERRGIDVKGKIVIARYGGSWRGIKPKVAAEHGAVGCLIYSDPRDDG
                     YFQGDVYPRGAYRNEHGAQRGSVADMPLYPGDPLTPGVGATKDAKRLEIKGAPTLTKI
                     PVLPISYADAQPLLRSLAGPVAPQGWRGALPLTYHLGPGPAVVRLKLEFDWKLVPAYD
                     VIARLRGSERPDEWIIRGNHHDAWVNGADDPLSGMVALMEEARAVGELAKQGARPKRT
                     IIYAAWDGEEPGLLGSTEWVEAHAEELRRHAALYVNSDSNGRGFLFIGGSHTLERFIN
                     EVARDVTDPQKKIPVLERARALRIDDGSADDRREARERADLRITALGSGSDYTPFLQH
                     LGVATLNLGFGGEDGGGSYHSIYDSFDHYTRFGDPNFDYGVALAQVGGRTVLRFANAE
                     VLPLSFTHLAETIGRYVREVTKLADDTREEIAEGNRRITDKTFEAVFDPTEAYVAPKP
                     KPPAPHLNFAPLQNALARLQESSRKYHAALGEPAARERLARTPQTRNELNAVLMQIER
                     AMTRDAGLPRRPWFKHQIYAPGFYTGYGVKTLPGVREAVEQHDWREADEMILVVAGTV
                     EHLAETIGRYVREVTKLADDTREEIAERNRRITDKTFEAVFDPTEAYVAPKPKPPAPH
                     LNFAPLQNALARLQESSRKYHAALGEPATRERLARAPQTRNELNAVLMQIERAMTRDA
                     GLPRRPWFKHQIYAPGFYTGYGV"
BASE COUNT         4493 a         8485 c         7680 g         4169 t
ORIGIN      
        1 ttggcctttg agaagggtct taatttcccc ttctacatct acaacgaaga cggcacgaac
       61 cggcgcgaga acatcaccga ctgggcgctt gagaagttcc acgcgcagta cggcgacgcg
      121 tcgatcacga agtgggacat ctttcattac gtctacgccc tgctgcatca ccccgcctac
      181 cgcgaacgct acagtgcgaa cctgaagagg gaactgccgc gcgtcccgtt cgcgcccgac
      241 ttccgcgcct tcgccgaggc gggcgcgcgt ctcgccgaca tccacgtcaa ctacgaacgg
      301 cagcccgagt acccgctcga aagaatcgag aacccgcgcg agccgctcaa ctggcgcgtc
      361 gagaggatga agctcggcaa ggacaaaacg cagatcgtct acaacgactt cctcacgctg
      421 gcgggcgtcc cggccgccgc cttcgagtat cgcctcggca accgctcggc cctcgaatgg
      481 gtcatcgacc agtatcaggt cagcacggac aagcgaagcg ggataacaaa cgacccgaat
      541 cgcgccgacg acccgcagta cgtcgtccgc ctcatcgcac aggtcgtcaa cgtcagcctc
      601 gaaaccgtcc gcctcgtgcg cgccttaccc gcgctcgggc tggcggagtc tcggaaggcg
      661 ggagacggtt cccaaagcct ttgacattct ccctccacct gacgtaaaag cacttgcacc
      721 aaacatctct tcggcatagg aactttcagc atccaccgat ccccgacctt acagggccga
      781 aagtaaacac tgatgtcctt tgaagagatg ctctcattca atcgccgtcc cgctcgcgcg
      841 gtgagtggga attatcttgc cctcggaaag gattgactca catgaacaac ttcgtacctc
      901 gtcttctcgc gttggtaaaa gtctctctgg cgctggccgc cgcgaccgtg ttgctcgcgg
      961 gcgcgcaggt cagcgcgcag tgccccggcg tcacgacggt gactgccggc ctccggctcc
     1021 cgctcgggat cacccagtct aatcaagata acctgctcgt ctcagagacc ggctttggcg
     1081 cgccgggcac gggccgcatc tccatcgtcg gccccggcgg cagccgccgg acgcttttgg
     1141 acggcctccc ttcggggatc aacgatgtcg gcgatccctc cggcccggcc ggcctctccc
     1201 tgcgcgggcg caccctctat gtcgccatcg gtgtcggcga tgtgggggtg gccggcccca
     1261 ttccggggac gacggtgccg aacccgaacg gcccgtcctc gccactcttc agctcggttc
     1321 tggccatcca cttcagcgcc cacgtcgaga agaccacggc ggggtttacg ctcaccgccg
     1381 cgcaacagca ggcgctcgcc ggcggccaga aggtgacgct gtcgaacggc ggcggcgaca
     1441 aaatcactat cgagctggtc gttgattttc ccaactacat atccaaccca ctcccgttct
     1501 tcgcggacaa catccgcctc tcgaacccgt tcgacctggt cgccgtcggc ggccagctct
     1561 acgtcacgga cggcggccgg aacctggtct ggcaggttga catcgattcg ggcgcgtcct
     1621 ccgtgctcgc aaacttcccg ccgctcgcca acccgctgcc cttcggcccg cccttcatcg
     1681 acgccgtccc gaccggcatc gcctactccg acggccagct cctggtgacg ctgttccgcg
     1741 gcttcccgtt cccgccgggc gcgtcggagg tgcgtgcggt tgacccggag acgggagctc
     1801 aagcgccctt catcaccggc cggtcgtcgg ccatcgacgt gctctccttc aggagtaaag
     1861 gcgacacgga ctacctggtg ctcgaattca gcgccgacat gctcgccggt gatccggggc
     1921 gtctgctacg attcgaaacg cccaccagca cgccgaccgt cgtcgccggc tgcctcatca
     1981 cgcccacctc catgacgctc gacgagaaga ccgacacgct ctacgtcacg gagttgggca
     2041 cgggacgtgt cgtcagcatc cccgtcgccc cgtagccggg ccgggtcgtg tttatgtgtc
     2101 catcagtggg cggccggaca agctttcggc cgcccctctt tttcggctca gacgaggggc
     2161 gcacagaccg gcgcgcgcgg ggccttctcc gcgcgccggg gcgtgttcgc gcccgtcgcc
     2221 cgaatgggat aatctaaact gccgtgaaca accctttcgt cccgacggtt ctgcgcttcg
     2281 attcgctgcc ctcgaccaac acggaggcgg cgcggcaggc ggcggccggc gcgccggagg
     2341 gactggtcgt cgtcgcgcgc gagcagacgg cggggcgtgg gaggcagcag cgcgtgtggt
     2401 cttcgccgcg cgacgcggga ctctacctga gcgtgttgct tcggccgcgt ctgcccgccg
     2461 tcgagtggcc gctgctgacg ctcgcggcgg ccctcgccgt ctccgacgcg ctcgaagaga
     2521 cgtgcgcgct gcgcaccgac atcaagtggc cgaacgattt gctcgcgggc gggcgcaagc
     2581 tctgcggcat cctcgccgag acggtcgagt ccgagcgcgg gcgcgcgtgt gtcgtcggca
     2641 tcggcgtcaa cctcaaggct tccgcatacc cgcccgagct gcgcgcatcg gccacatcaa
     2701 tcgaggaggc gacgggcgcg cttgcgggcg acaactcttt gcttgcgggc gtcgattctt
     2761 tgatcgagag tctggtgcgc gccgtcgggc gtcgctacgc gcggctccag tcggagggcg
     2821 gggccagaga gacgcggcgc gagtggtcgg cgcgctcgtc ttacgcggaa ggccggcgcg
     2881 tgcgcgtccg actcgacggc gagagcttcg agggcgtgac gcgtgggctg gagccggatg
     2941 gggcattgcg cgtggagtca gaaggaggcg cggtcaggac ggtgcgcgcc ggggacgtga
     3001 cggctctgag ggcggcgctt tgaagacgcc ggccagagcg cgcttaagaa tgccggcccg
     3061 tcgggcgaag taaatccggc tcatcgtcgt cgtcctcagg ctcctcttcc ggcgggaaga
     3121 cgacgcggtc gtaaacgtcg gcgagctgaa gcgtgcagtt gattgagtcg aggcgcagga
     3181 cggcttcgag gccgagcgcc ggcgtgtaaa tccacggccc gtcgggcagg cggccgaagt
     3241 ggtcgatgtg cggggcaatc tgggagacga ggacgtagtc cgtgagagtc ggcagccacc
     3301 tctggtagcg cagccacttc tcgccgcggt cgaaagcctc ggtcgtgggc gacaggactt
     3361 caacgatgac ggatgggttt agcagcacgt cgcggtgctc gtcaatgtgc ttgagttcgc
     3421 cgcagacgac tacgagatcg ggataggagt aaagcccctt cgacgagcgc cgcgacttcg
     3481 gggcggggcc gctgcgaact ttggtgtctt tcgagaacgc ctgacagggc gtacctcgga
     3541 gttgggcgta aagctgcccc gcgatgttga tgcagatggc gccgtgcgcg gggctttcgc
     3601 cggccatctc atagatgacg ccgtcgagat actcatatcg ttcttcggac tcgcgttcaa
     3661 tggcgaggta ttcttcaata gagtaaatga gttcggtccg cggtaagctc atcggcgctc
     3721 ggcctccgtc attaaggggt tgcatgagca gcatcggcgc ccatgattat agcctgaatc
     3781 cttcgtaaca agctcgacca atgacaggga aacgcatcat cctctcgacg ttcggctcct
     3841 tcggcgacgt gcatccgtac atcgccatcg cgctggagtt gaagcggcgg ggacatcggc
     3901 ccgttatcgc cacgacggag atgtaccgcg agaagatcga gccgctgggg ctggagctgc
     3961 accgcgtgcg gcccgacatg ccctcctacg acgagccgga gcgcgtggcc gcgatgatcg
     4021 agtatgtggt cgatgagaag cgcgggccgg agcgcctgct tcagacgatg atcgtgcctc
     4081 acctgcgcga cgcttacgaa gacctgctgg cggcgacgga aggggccgac ctgctgctca
     4141 cgcacccgct gccgttcgtc ggcccgctcg tcgccgcgaa gacgggcgtg ccgtgggttt
     4201 cgagcgtgct cgcgccgggg tcgttcctct ccgtctacga cccgccggtg ccgccgcagt
     4261 ttccctcggc gcacaagtta atggccgcga gccgacatct cagccgcgtc gtgatgtggc
     4321 tcgcgcgcaa aaagatcgac gcgctcctcg ggccgatcta cgaattccgc cggcagctcg
     4381 gcctgccgcg cggcggcaac ccgatgatgg agggccagca ctcgggcgcg ctcgtcctcg
     4441 cgctctactc gaaggtgctc ggcgcgccgc agccggactg gcccgcgaac gtgcgcgtca
     4501 ccggctttcc cttctacgac cggcgcgacc gcgcgggcga ccccacaggt ctcgcgcccg
     4561 agctggaaaa gtttctcgac gcgggcgagc cgcccgtcgt cttcacgctc ggctcgtcgg
     4621 ccatctgggt ggccaaggat ttttacacgg agtcgatcaa ggcggcgcgc gctttgggcg
     4681 tgcgtgctct gctgctcatc ggcgacgagc gcaaccgtcc ggccgacgtg ttgcccgagg
     4741 gcgtggccgc gttcgactac gcgccctatg gcgagctgct gccgcgcgcg cgcgcaatcg
     4801 tccatcaggg cggcgtcggc accacggggc aagccctgcg cgccgggcgc cccacgctca
     4861 tcgtcccgca cgccttcgac cagcacgaca acgccgagcg cgccgcgcgc ctcggcgcga
     4921 gccgcacgct gcggcgcaca cgctacaacg ccgacgcggc ggcgcgcgag ttgcgcgagc
     4981 tcctgggtgc tgagtcttac gcgagacgtg ccgaagaggt cgggcgcgtc gtgcagtcgg
     5041 aggacggggg gcgcgcggcg tgcgacgcga ttgaggaaga attgaatcat tgattcgtcg
     5101 gttcaatgaa gtagtggttc atgctttcat ggaaccgatg aatcaatgat taaacgaatc
     5161 aatattttcc gccctcccgg tctcgtgtta tcttgtcgcg gctctgaatc caatccacta
     5221 tcgcggagaa gacagatgac gaccgacgtg ctgacggaag aggcgttcgc ccgcaacttg
     5281 aacacgacgt ttcgcgtccc cctgggggac ggccggaagg tcgagctgga gttagtcgag
     5341 gtggccggct acaaggacag gcctgacgag cagcagggga tggaacgctt ctccctattc
     5401 ttcgaggggc cggccgagcc gttcctgccg caggccagct acacgatgga gcatgagcgg
     5461 atgggcgagg tactgatctt catcgtcccc atcgccagca ccggcggggg cttccgctat
     5521 caggccgtct tcaacttcca cagaaagtga acggtgacga ctactcgctt actcccttgg
     5581 cggaggctgt gcacgataaa ttaaaggatg tgaaagcgta tgagactgat cttcacagcc
     5641 tccctacatc tggtcatcct cctcgcctcg atttcgccgc aggcacagac gcgcccgcgc
     5701 ggcacaagga gaccgcctcc tcgacccccg acaacttccg tgaccagacc ggagtcggtc
     5761 ggcgaggatg aagtcctgcg ggtgacgacc aacctcgtca ccgtccccgt cagcgtcagg
     5821 gggcatgacg gcagctacgt gtttgacctt aagcagggag acttccgcat ttatgaagac
     5881 ggcgtcgaac aggagataac ccacttcagc agcgtcgagc aacccttcac cgttgtgctt
     5941 ctggtggaca cgagctcctc gacggagccc aacctcgggg agatcaaaga ggcggccaac
     6001 gccttcttcg gtcaactgcg gcagaaggac tccgtgctgc ccgtcacctt cgacgggcag
     6061 gtacgcccgc tcttgtccaa gggcacgagt agtcgaaccg ttttgcgcga cgccgtcgaa
     6121 agagcgcgca cggacacggg gaacaacggc accaaacttt acgacgccgt cgattatgct
     6181 tatcaagtgc tccggcgcgt ttcaggcagg aaggccatca tcttgttcac ggacggcgac
     6241 gacacctgga gtagtgcgac gatgaggagc acgctctgca acaccgcgga acttgacgcc
     6301 ctcatttacc cgatccacta cggctcttcg ccctccacga attaccttcg ggccttggcc
     6361 agcgagacca gcggacgcta ttaccaggcc gacaacgtcg aaatgatcaa acaatccttc
     6421 gccgccgtgg ccgaagagtt gcgccggcaa tacagcatcg gctattaccc caaagcgact
     6481 tcgcgtcacc agcaggagcg gcaacttaga gtagaggtta aaggcccggc cgcgggcgtg
     6541 acgacgcgca agtcttacct ctaccggccg tgaggtagac acaatttccc gtcttaccaa
     6601 tcggccttat cgggtcgctt gcctcttgat gcgtttacgg tcttcggcgg ctgaaggggg
     6661 cagcgtcccc tccctcctgg aatggcgccg cggcgcccga cccgcaggtc gcgccgtgag
     6721 ttcggaactg tccacttccg gcaggcttcc gccccccgcg tgcgcggccc ttcgggcgcg
     6781 aatgccggat tcgctgcttg cgatgaatac cactcagggg ttgaggcggt taattaccgc
     6841 atgatagtac aaccacttct tgtccttctt gccggggact ataaagaggt cgaacgaccc
     6901 aagcgcgtct tgctctagca ggtaggttct ctgcgggagg ggttgattta gggggccact
     6961 aaatctgagc acgaagcatt cgcgccccgc caccgtttga tcggggacag gccctatgtt
     7021 ggagacctca atcagtgtca actccaccgc gttcgaggct ccggcgttga ttcggaagac
     7081 cgtgttcagg tttgcctgaa acgtcgcctt ggtgaaattg ttgagcggat cgaggcggct
     7141 ctcaggtggg atggggaatc cctggccgga gtccgaaccc ccggtgccgg tttgttgtcc
     7201 gaatgcgagg tcggtgacgg ataaagaaac tccggtggcg aggacgagca tggtccctcg
     7261 cttcataaaa tcgcgccgtg aaacaatcat tgggggctcc ttagcttact cagatgtctt
     7321 tagctgttgc gtaaggtggt atgcgtgagg ggcttggaac ttctatcaat ttaatgcggc
     7381 ggagtcaaat attttcttgc tcgggacctg acgaaggatt acaatcgcga ccctgcgctt
     7441 ctgtcaggta caaagttttg gcagccgctc ctcctatcgt cacacgcagt acttctgagg
     7501 tgatagccct ctcgccagtt tcgacttttc tccttcaggt tggctttctg tgacgaccgg
     7561 cgacggctca ttcaaccata accgggcgct cttcggcctg cccggcacgg ccttcggcgg
     7621 cggctctttt cgctcagcat cgcgcggcgc tgtttcagtc gcaccgcggg ggtgcgactg
     7681 aaactccagg tgaggatacc taacgatgcc ctttactcgc aggcagagac gacgcctcaa
     7741 gtggaagacc ctcacaaccg ggaaacccct ggtgtgtgcg ctactgttgg ttatcggctt
     7801 cgggttggcg gtgtccctcg ccggccctct gggaggggag gtcaggggaa cgatagttgg
     7861 tctcgctgac tatttcactg acggcgcggg taaagataaa gacaagcctc cgcacactcc
     7921 gcccggctcg tcagccgggc agacagcctc tcgggcgaca ggcgattcgc catccggccc
     7981 cgccttccgc atctcgcgag catcggcggc tggacccaac cgcaatgtca agtccgcgga
     8041 tgtcgcgtac aaccgtgacg acaaagagta cttagtcgtt tggcagggcg acggcctcaa
     8101 cggggccaac ctcaggggtg tggatgaaat cttcggacag cgcgtcaacg ccgcgacggg
     8161 tcgtgaaatc ggccccaact tcctcatctc gaacatgtcg gtttgcggga cggattgcga
     8221 cgccaacaaa ccgcggatcg cttacaacgg cgccgcacgc gaatatctgg tcgtctggca
     8281 cggctccggc ttgatcgaca tgccgggtaa gatttctgag gtctacggcc agcgtatcag
     8341 ccgcggcggc gacgaaatcg gcagcgattt tcgcatctcc aacacgacgg acttgggcaa
     8401 agtgaacggc aacttcgtgc gggcgagcgc gcaggccgac gtcgcctgga acagcgtcaa
     8461 caatcagtat ctcgtgattt ggagcggcat ggggcagccg gaagacgtcg tgaaactgga
     8521 gatttacgga caactcctga cttccggtgg cgaagccgtc ggcaacgatt ttcgtatctc
     8581 caacacaacc gaacagggca acaacttcca cgccagcacc cccgcggtcg ccttcaacag
     8641 taccaacaac cagtacctgg tcgtttggac cggcggcttt aaggaagagt ctcgaaccga
     8701 ggtttggggg cgggccctcg ccgcgaaggg cgggccgctc ggcgacggtg atttccgcat
     8761 ctcccaggtc agcacggccg ccggcagcaa ccggcgcgcc ggttcgcctc atgtcgtcta
     8821 caacggtagc aacaatgagt acttcgtcgc gtttcacgca aacgggttgg cgggcgaagc
     8881 aaatgaaggc gtgaatgaaa tcttcggaca gaggatagat gcggcgaaac tgaccgagac
     8941 cgggcccaac gacttccgcg tctcgaacat ggggggcgcc gggaatggag cgataaggcc
     9001 gcgggtgatt tacaacagtt tcgataaaga gtatttggta atctggcggg gcgttcgggc
     9061 taacgtcccg cctgaggttt tcggccagcg ggtgagcttg agcgggactg aaatcgacgc
     9121 ggacttccag gtctcgaata tcgccgcggt cgggaaggat cgctcagtca acgccgccgt
     9181 cgtggctcac ggcggcgacg gcggcgagta tctggtcgtg tggcacggca acgcgctgcc
     9241 cgacgcgacc ggcaaggcca ttaacgaggt cttcggccag cgagtgagga tcatgccggc
     9301 gcggggcacg cggcagtgat cctctccgcg catggaggct gtcgaaaaaa gttcggaagt
     9361 aggaaatcaa atcgttgaca agtcataggc aagaaactcc cgggagccgc ttctcgaaca
     9421 acggctcctg ccggggccta tctgccgcct ctcactgagc gaacacgtta gtgccgactt
     9481 tcattttatt cttcttgaaa atttcccccg caccggttac aatcccgccg cttcgcactt
     9541 caaacaagcg ctgttcggtt ctacaagcag acaatacggg acgaatctcc accaccggca
     9601 tttccgtaag tacacaggtc tgagtctttc ttacacacct cgacttctcc gttcggcatc
     9661 gcaagctgaa tcggacgcac ccaccaagcc ggttgacaac aaatgtggga agctaaatcc
     9721 actaaggagt gaactatggc cgaacccttt ctcagtgaaa tcagaatcat gagcttcgta
     9781 tttgccccga agggctgggc tctctgtaat gggcaactgc tacccatcaa ccagaatcag
     9841 gccctcttca gcctgctcgg cacgaccttc ggcggcgacg gtcgcgtcaa ctttgcgctg
     9901 cccgacctgc ggggccgcac gcccatccac gtcggcagcg ggcacacgct cggcgagagg
     9961 ggcggcgagc aggcgcacac gctcagcatc gccgaactcc cgacgcacat ccatacgttg
    10021 caagcttccg ctaacgacgg caccacgctc gtcccctcca gcaacgtcgt gctggcgaag
    10081 cgggccgccg agatttatca cgccccgacg aacctggtgg cgatgaacgc cgcctccgtc
    10141 cccaacgtcg ggggcagtca ggctcacttg aacatgcagc cgtttctgac cctcagtttc
    10201 tgcatcgcgc tgcaaggaat tttcccgtcc cccaactaag gaggcacaca tggcacagcc
    10261 atacgtaggc gaaatcagaa tgtttgcggg caacttcgcc ccagcgggct ggatgttttg
    10321 tgagggccag ttgctcccca tctcggagaa tgagacgctg ttccagttga tcggcacgac
    10381 ctacgggggc gacggtgaga gtaccttctc gcttcccgac ctgcgcgggc gcatccctat
    10441 ccaccagggc aacggcttca tcctggctga gactgggggc gccgaggaga tcaccctgac
    10501 ggtcaatcaa atcccggcgc acacgcaccc gctgctggcg tcggccgacc aagccaacac
    10561 tcccaaccca cagggcaacg tgctgagcga gacggtcaca accaccccct acttcaacga
    10621 cgtgccgaac acgaacctga accccgtttc gatcacaccc gttgggggca gccagccgca
    10681 caccaatttc cagccgtacc tttgcatcga cttcatcatt tcgctgttcg ggattttccc
    10741 gtccccaact taaggagaac cgttcatggc agacccattt gtcgctgaaa ttcgcatctt
    10801 cccttttaac tttgcgccga agggttgggc gtggtgcaac ggccagttat tgccgctctc
    10861 gcagaacacg gcgctcttct cgctgctcgg caccacctac ggcggcgacg gcaagtcgac
    10921 cttcgcgctg cccgacttgc agggccgcgc gccgatgcac cccggacagg ggccgggact
    10981 ttcgctccac gacctcggcg agaccggcgg gtcggagacg gtctcgctgc tcatatccga
    11041 gataccggcg cacacccacg ccctcagggc ttcttcggag atcggcgagg accggacacc
    11101 cgccggggag gccatagcac agtcaacagg cgggttcctc tatcgcaccc cggcagatgg
    11161 cctggtgcct atggcttttc aagggctgcc gccggcgggc ggcgaccagc cgcacaacaa
    11221 cctacagccg tacctgacct tctacttcaa catcgccctc cagggcgtct tcccaccgcg
    11281 cacatagaag gtagccggta gaaggcagcc gggacgttgc tacgcacgga acgtcccggc
    11341 taacttgttc tgccggcact cgcttcctgc gcggcagccg tatgtgaaaa cttgaaatcc
    11401 gctgccccgg tagaaattga cctcgaagga ccatgaaaac gccacgccag gtgtcagcgc
    11461 aagcttgagt tttatgcaca acaattttcc tggttggttc gctttgtgcc ccgggtcatc
    11521 gaacaccagt ctgcacccca cggccccact tcttctttcc ttatcacggt taaaaaatcg
    11581 ctgccgcttc gggaaaatcc ttgttgggga gatgggttct gtgatagtct tcgcctgaat
    11641 tttagaggaa gtaggccctt tccgttctcc tcgcaactcc gtcaaaggac tttccgggac
    11701 ggctcgcagg agcgcttgcc cttcctgatt ttcgctttac ctaatcccgt tacttcatgc
    11761 tcgctagcat cgactttagc tcgattctaa cgcgccaact ctcaatcgcg cctttcaaat
    11821 tggcccctta cctcaaagga tattccccca tgaagtctct tactgccccc gcgccccaca
    11881 gcaacgccag agcttatttt gccattctga tctcgttctt cctcgtcgcg ctgccggtca
    11941 cgccggtcat ggcggcctcg gcagcctcgg cggcccctgt cggctctgcc aaaccaaggc
    12001 cggagcccat caggagtgat tttaagtcgg cggattcaga cgcgggcggt gaagccgttc
    12061 cggcggaggc gctcggcgcg ccgcccgtcc ccgcggcgct cgcgcccgtg gtcacggcct
    12121 ccaaggctga ttcgttcccg gcgggcgggg acgttgataa cgatgggaag gctgatcaag
    12181 gcgacacgat tgagtacacc gtcaccatca acgcgagcgg cgaaaacgcc acgggcgtct
    12241 ccttcagcga cacgattgac ggcagcacca cgctcgtccc cggctcagtc acggtgaccc
    12301 cggtggccat caacgacagc tacattgcga cgggcaacgt gcgtatccag gtgccggcgg
    12361 cgagcggcgt gttgctgtcg ggggacgacg actacctcgg cgccccggcg gcgaccctga
    12421 gcggcttcgg gaacgcgctg ggaaatgcca acggcacggc ccccggtggc acggtgacga
    12481 ccacgaacgg cggcacggtc atccttgcga gcgacggcag cttcagctac aaccccgggc
    12541 ccggcttcga gggcgccgac aatttcttct acacgctgac gaactcggca gggagcaacg
    12601 tcgggcaggt gaccatcacc gtcagcgaca tgatctggtt cgtcaacgac gacgacccga
    12661 cgccgggcgg cgacgggcgg ctcactaacc cgttcaactg cctggtcggc gcgggctgct
    12721 tcgaccaggt ggcggccgac ggcgctggcg acaacatctt cctttacagc ggcagttaca
    12781 cgggcgggct gacgctcctc aacaacgaga cgctcatcgg gcagggcgcc ggtgacacgc
    12841 tggcgaacat cgccggcatc accctcgcgc cgcacagcga cgccttgccc aacacgggcg
    12901 gcgcccgccc aaccgtctcg gccggcagcg gtaacatcac cctcgggacc ggcaatctgc
    12961 tccgcggctt caacatcggc aacagcgggg ccggggcggc gatttctggc accaacttcc
    13021 tcgcgctggc ggtgagcgag atgaccatca acggcacggg ccaggcgctc aacctcacca
    13081 acggcactct caatgtgtct ttagacagca tcacgtccac cggcggcagc aagggcgtca
    13141 gcctcacgac catcaccggc ggcagcctca ccagcggctc gaccaacgtc cagaccccga
    13201 gtggcgtggg cgtggaagtg ataggatcgg tcgccaacgt cagcttcgcg aacaccactg
    13261 tcaacggctc gggcgggacg ggcgtcagac tggataacaa caccggcaac gtcagcttcg
    13321 ccgacctcga catcacgccc aactcgggcc agcgcgcgct gtacgcgacc aacaacgcca
    13381 tcagcagcgg cagcattacg gccaccagtg gcacgatcag cacgaccaac gcggtcgccg
    13441 tcgagatcac gcgcgccagc ccgaccacct cgctgagcat ggcgctgacg agcgtctcgg
    13501 cgagcggcgg cacgagcggc atcgtcttgc agaacacgtc gggcagcttc gccgtgttgg
    13561 gcaccactgc cggcatctgc ggcggctcgg tcaccaacac cacgaacccc gccgtcgccg
    13621 ccaccgtcac cgcggccaac accgccgact gcacgggcgg gacgattcag aacatgacgg
    13681 ccgacggcat ccgcctcaac aacgccagga acgtctcgct cacgcggatg cagatcgtca
    13741 acggcgctca gagcggcatc ttcggcaccg cggtcaacgg cttcgagctg atctcgtcct
    13801 tcatctccaa caacggcaac gccgccgacg aggtcggcct caagttcacc gacgtcgtca
    13861 accccggcac cgtcaacggc ctcgtcggga cggccctcaa cggggcgaac ccgacccgga
    13921 tcatcaactc gacggtgcgg cagtcgggcg agttcaacgt cgaggtggtc aaccgcacgg
    13981 gcacactcac cgacctgttc gttgacggct gccagttcac cgacacgaaa ctcaacgccg
    14041 ggccggccga cgccgacggc ttcaacgtcg agatgcagaa cacggcggtg gcgacggtgc
    14101 ggctccagaa cagcgtcttt acgaacaact tcacgcaggg ggcgcagctg tcggcgattg
    14161 acagcgccaa cctgacggcg acggtgacga gcagcacgtt tacgaacaac aacgaggggt
    14221 tcgtctgcaa ccactcgggg aactccgacc tcgtctgcac ggtgggcggc gacacggccg
    14281 cgcagggcaa ccacttcgag ggcgagcccg gcggcgccgg cactgacacg ggtgcgctga
    14341 tcgtggcgag cacggggagc acggggacgg tgagcgcgag cctgacggcg aggatcaagc
    14401 acaaccacgt gacggcggcg aacaatcagg tcaaccactc gatcatcgcc ttcatgagcg
    14461 gggtggggac gcccggctcg gtcaacatca gcaacaacac ggtcaaccat caggtcttcg
    14521 ggcacgccat cctggtggac acgcccgaca acgccgccag cccgaatttc ggtgtcacgg
    14581 cgaacaacaa cgcggtgacg aacaacgctc cgggcgggca cggggtgctc gtgcaggcgc
    14641 ggcagaactc gacggcctgc gtgctgatcc agaacaacac gggcacggtc gcggccggct
    14701 cctcggtggc gagaatccgt cagaactccg tggcgggtca gaattcgacg ttgaatctca
    14761 aacagggcgt ctcggcatcc aacgtggcga cacaagtcat gatcgacaac aacccgggcg
    14821 tcatcacttc cgtaaacggg gcaatcaacg tggtggctaa cggcgcttgc acgacgccga
    14881 cgctggcgcc cagcaccatc gaaatcacta agacggagag cgtccccagc gagataggaa
    14941 agcgcggcgc acatgcgacg gaggacgcca cgccgacaag cagcatgggc ggcgtcacca
    15001 gccgtccgtt cgtctcgatg ccgagcaagc aagcggcggc cccggccgcc tctgcccccg
    15061 cctccccggc gacgcttgcg gcccccgcgc ccgtggcgct tggcggcggc ggcgacgaag
    15121 aggccgagac gcctgcgtcc ggccccaact ccgtcggcga cccgctcggt tcgggcggca
    15181 ccgtctcggt caacatcggc accctcgccc ccggcgactc ggtcacgatc accttccagg
    15241 tggtcatcga caacccgttc accggcaacc ccgcgcaggt gtcgaaccag ggcacagtct
    15301 ccggctctaa cttcgccaac gtcttaacgg acgatccatc ggtgggcggc tcggcggacc
    15361 cgaccgtcac gctcatcgcc gtcccgcccg acgtcttcat ccgcgacggg cgcatcgccg
    15421 agccggccac gggttcggcc aacatgatct tcaccgtcgt gctctcggcg cccgccaccg
    15481 gcgccttgac cgtcaacttc accacggccg acgagccggc cgggccgggc aaggccgtcg
    15541 ccggggctga ctacacgacg acgagcggct cggtcgtctt cgccgccggg gagaacctca
    15601 agaccatctc ggtgccggtg ctctcggacg ccagcgccga gccggacgag accttcctcg
    15661 tcaacctcac gggggcgacc ggcagcttca ccgccatcct cgacaatcag gccgtgggca
    15721 ccatcacggt gaacgcgccg ggcaccttcc tcatctcgga ggtgcgcact tcgggtccgg
    15781 gcggcgcggg cgacgactac gtcgaggtct acaacaactc cgacacgccg ctcacggtgc
    15841 agaccaccga cggctcggcc ggttacgccg tcgtcgcgac caacaacggc tgctcgggtg
    15901 atcccgtcat cctcggcatc atcccgaaca cgacggtcat cccggccagg ggccacttcc
    15961 tgttcgtcgg gccgacctac tcactcaccg cggtggccgc gggcaatctg acgctgacgg
    16021 cggggatcaa cgacgacgcc aacgtcggtc tgttctcgac cagcacgctc attaacctct
    16081 cgtccgtggc caagctcgac ggcgtcggct tcggcctgaa cacggccgac ctctgtgacc
    16141 tcatacgcga gggcgcgacg ctgccgaccg ctcagggcag cacgcagcag tacgccttcg
    16201 tgcgcgagtt caccctggtg gccgtcaacg tgccgacgcc gaccgacgcc aacgacaacg
    16261 ccacggactt caccgtggtg tcgacgacgg cgtcgacgcc ggtgagcgcg gccatcacgg
    16321 cgccgacttt gggagcgccg gggccgcaga acatggcgtc gccgaagctg aagaagtact
    16381 cgcaggtcgg ggtgcagtat ctcgacccga cggtggcgcc caacctcgcc ccgaaccgcg
    16441 tgcgcaaggg ctgcgccact cccggagtcg aggagtgcct gcctaaccgc tcggcgcagg
    16501 gtacgatgca tttgcgccgc acgttcacca acaacacggg cggccccatt tcgcaactgc
    16561 ggttccgcgt ctacgacatc accgggttcc cggtgacggt cgcgggcgac gccgacatgc
    16621 gcttgatcaa cgcgccgcag gtgactgggg tgacagggcc gtcaatcggg acggtgacgg
    16681 tcgaggcgac gacgctggat gggccgaccc agacgacgtt cggcgggggt ctgaactcgt
    16741 cggtgacggc cgggatcatc acgctgggca accagctcgg caacggcgcg tcgatccaca
    16801 tcaacttcat gctgggcgtc cagcagacgg gcaactatcg cgtcttcgtc atcgtcgagg
    16861 cgctgccgta acacgcgcgg ccgcggggct gcttaaccac gaacgggccg cgctgtctca
    16921 ctcgggtgag cgcgcggccc gttctcctga aggcaaaaag gtaaaaggca aaaagttaaa
    16981 aacggacttt atccttttgc ctttatcctt tggcccttcc gaaaactcat gcttctggtc
    17041 atcgatgtcg ggaacaccaa cacgtcgctc ggcgtctacg agggcgagcg gctcgccgcg
    17101 cactggcgtc tgacgacggc ccgcgagcgc accgtggacg agtacggcgt ccatgcgcgc
    17161 aatctcttcg cgctcgccgg cctcgacttc aaggccatca cgggcgtctg catcgcctcg
    17221 gttgtgcccc cactcaactt taccctcaag cggatgtcgg aagactactt ccacctcacg
    17281 ccgctcttca tcgaccacgg aacagacgcc ggcctcgaaa tcctctacga gccgccctcg
    17341 gacgtgggcg ccgaccgcat cgttgacgcc gtcgcggcgc tgtcgaagta cggcgcgccc
    17401 tgcatcgtcg tcgatttcgg cacggccacc accttcgacg ccatcaacgg gcgcggccag
    17461 tatctcggcg gcgtcatcac gcccggcatc accatctctt ccgacgcgct gtttgcgcgg
    17521 gccgcgcgtc tgccgcgcgt cgaaatccgc cgccccgagc gcgtcatcgg ttcttccaca
    17581 gtcggctcca tccagtccgg cctctactac ggctacgccg gcctcgtcga tggcatcctc
    17641 cgccgcatga tcgacgagct gcggggcgag ggaagcgccg agggtgagcg cgtccgcgtc
    17701 gtcgccacag ggggcctggc cccgctcatc gccacggcct ccgaactcat cgagaccgtg
    17761 gacgacacgc tcacgctcga cggactgcgg ctgatttatg gccgagttca aagttcaaag
    17821 ttcaaagttc aaagcccgga agccgacacc gcataacgag gctggcgcgc acgccgcccc
    17881 caagtttaaa ctgcgaaccc gcgcgccggc gccgccccga actttgaacc ttgaactttg
    17941 aactttgaac tacttcaatt actacaccga gatcgaagac acgttcgtca ggcgaagggg
    18001 gaagcacctg cttttaagcc cgctcgactg ggcgctgatc gagagctgga gaacgatggg
    18061 cgtgccgctg cacgtcgccc tgcgcggcat cgagaaatcg ttcgactcct acgaggctcg
    18121 cccgcgcaac cgcagcgtca agtctctctt ctactgtcag gaggaggtcg aggctcagta
    18181 cgccgactgg ctggagatgc agcggggcgc ggccgccctt ccgggacaga ccgtctccgc
    18241 cgacggcgtc gtcaccgctg ccggggcgac ggcggaaggt gagggccggg aggttgagac
    18301 atcagcgcgt ctgccgttcc cgcgcgccgt catcgccgga cacctcgcgg agagccgcgc
    18361 cgctgtcgaa cgcgcagccg ttgagcgacg cggaggggcg ggggcggcgg gcgccgacga
    18421 actctccgag gcattggagc gcgtgtcctc gcgtctctcg gagctagagc gcgacttcgg
    18481 cgcggcggcc cgccccgacg ccgaacagct tgaagccgcg ctcaccgacc tcgaaggtct
    18541 catcgaccgc gccgtgcgcg ccggcatcgg ggccgacgcg ctcgcgtccg caagggccga
    18601 agccgagggt cagctccggc cctatcgcgc gcgcatggac gtggccgtct acgagacgac
    18661 gctcgaaaac attctggcga aggcgttacg cgaacgcttc cgcctgcctc gcctgagtct
    18721 gttccacctt taaaccaagg ccgcccatcg ggggccgccc gaaggagagc gaaacgaaat
    18781 ggcaaacatg atggtcaggc gcgtcggggt gctgtctctg gcgaagatca gcggcatcgc
    18841 cggcgcggga ctgggtctca tcatcggggt catctacggg ctgctgatca tggtcatcgg
    18901 ggcgacggcg ctcgccggcc gcaacggccc cggagccggc ttcggcatcg ggttcggcct
    18961 cctggtcatg atcatggtgc cggtcatgta ctccatcttc gccttcatca tcggcgccat
    19021 ctacgcgctc atctacaacg tcgccgccgg cttcgtcggc ggcatcgagc tggagcttga
    19081 gccgacggac accggttaca gccaaccgcc cccgccgccg cagcagcagt gggcgccgcc
    19141 tccatacggg caggcgtcgt ccgggcctta cgacgcgagg tagatgaaag accgcaggtc
    19201 acgacagaag agggcgaagc cgcatccaca agccgtgagc gcgggcgctc cggccgtggg
    19261 cgaggagttc gaggtcgagg tcgaacggat acttccgggc ggcctcggcc tcgcccacgc
    19321 ggccgggcac acgctcatgg tggcgctctc ggcgcccggc gaccgcgcgc gcgtgtgcgt
    19381 ccgacaggtg cgaggccgcg tcgggttcgc ttccatcgag gaactactct cgcccggccc
    19441 cgctcgcacc gcaccgccct gcccctactt cggtcgctgc ggcggctgcg acttccagca
    19501 gctcacctac gaggcacagc tcgccgccaa ggtcgagatc gtccgcgact gcctgcgccg
    19561 cgtcgcgcgt ctcgaacccc ccgccgacat tcacatcacg ccctcgcccg acccctggcg
    19621 ctatcgctca cgtgcccagt ggcaatacga cacggcctcc ggcgcgctcg gctacttcga
    19681 gcgcggcacg caccgcgtct gcgacgtggt cgaatccccc gtcgtcatcc cgcccctgca
    19741 agccacgctc ggcagcctgc gcgacgccga gcgcgacggg tctctgagcg cgagcgtcgc
    19801 ggagttccac gccgtcgccg gcgacgaggg agtgggactt gcgccccctg tcgaggagtc
    19861 gcacgcgcgc gagcagacgc gcaccatcgg cggcgagcgt tacagcttcg acgccgagtg
    19921 cttcttccag atcaaccacg cgctcctgga cgcgctcgtc cgcgagggcc tgcgcgacgc
    19981 ctccggccac acggccctcg acctctactg cggcgtcggc ctcttcacgc tcccgctcgc
    20041 gcgccgcttc acacgcgtct ggggtgttga gggcaacgcc cgcgccgccc gcttcgcgcg
    20101 ccgcaacctc gccgacgccg gtctggataa cgcgcgcgtc gagacggccc ccgtcggcca
    20161 gtggctcgcc cgccgcatca gtgaaccctt cgccgccgac ttcgtcctcc tcgacccgcc
    20221 acgcgccggc gccgacccgc agacgctcgc ctccctcatc gccctccgcc ccacccgcat
    20281 ctcctacgtc tcctgcgacc ccgccaccct cgcccgcgac ctccgcgccc tcacggagcg
    20341 cggctaccgc ctcgcctccg tccacgcctt cgacatgttc ccccagaccc atcacgtcga
    20401 aacagtcgtc cacctctcca ggagtgagta ctaaaaaccg tgccgggagg caagacgccg
    20461 gacgagtcgc tgtggaagaa aacaaacttg aatgtatccg aagcgaaggt agaatgattt
    20521 aagtcggcaa ccgtggtgat gggtcacaca cttctaaacg acggggtagc ttgctcggca
    20581 actcgctcta ttgaaatgac aactgccggc ccgtggcgat gtcaaaaatc acgtcgcgtt
    20641 ctctgggccg ctggacgtgc tggaattgct tgaggcgcgc ctcctcacgc acccggtcaa
    20701 cgccgatgcg gaagccgggg acggtgcctt cggcgatgag ccaggtttgc ccttcccatt
    20761 cgagcgagag gccgttgcgc gcggggaagc cgccctgccg gacggcgacc gtgatgtggt
    20821 cgggcgtgta gacgaacagg tagtcttcgc cgagctgctc cagtagcgag ccgaggagga
    20881 tggccttgtt gctgcaatcc gactctcccg tcatcaagac ctcgttcggc ctcttcaacg
    20941 tctcgaagtt gtagctggcc gcgcgcaggt cgtaggtgat ctcgcgcgag acgaaatcga
    21001 gcacgcgctg cacgcgcgcc tcacgcgcgc cttcgccgtc cgccgggatg tcgtgcgtca
    21061 gcccgcccac gaagcggcgc agcgaggtct cttcgggccg cgcgacgaac gcgccgtggt
    21121 tggcgaagac gacgggcagc cccgcccggc tctgtcccgt ccgcgcgtgc atccgcccgc
    21181 cgaagatact cttgttctgg aggaagtcgc tcaactcgcg catatcgagc gtgtagatcg
    21241 ctgacccgaa gtgaaacttg aggacagtct ccggatcgaa cctgacgtta tccaccggcg
    21301 tcttgaagaa aaagtgcttt tccgtcgaac gaaccagcga gtacttgccg aggcggagcc
    21361 ggcccgcgcc gtcgtcctcc gccagcacga cctgcttcag gtcaacgatg aagcgcgcga
    21421 gtatcgcctc gaacgtctcg cccgtctggg acggcgcggg ggccgagaag taaagctcgc
    21481 gctcaggcag ttgcagcatc tccagccgca cggggccgag cacctgctcg accactctca
    21541 tccccgcgaa gctgggctgt gcgagatagg agagcaccgt gtcgcgcgtg cgctcgtcga
    21601 gactgggcgg ggctaactcc ttcagcctcg cctccaccat gctgccgccc cggcgcgagt
    21661 cggcgcgccg cttgtagatg gcccaccccg cgccgcacag tgcgaccgcg aagacgacca
    21721 ccagaattag cttccatata ctcgaacccg tcggcacgtc gcgtccgagg gtgaggacgg
    21781 ggaagtcggg ccgcgccgat tccgtcgcgc tcgcttcggg gtcgccgccg ccgacgagcg
    21841 gcacgccgca acgctcgcag acttcggcgc tcggccaact gacgaggccg cagcgggggc
    21901 atttcgtgtt gctcattaat ttctccggtg ggattgaggt tcgcgtccgc gcagggtgtt
    21961 tggatttcga agacgtgagg cggcgcgccg aacttgcgaa atgactcttc tgggcgcgcc
    22021 cctgcgaaat atatctcccg aaaaaaggcg cgtcaatatc aatctaattt cgactcgtcc
    22081 accgtgccca ctgcgcatcc tgcttgatgc cgcgcgtgct ctgttataaa gtccgcttcc
    22141 gccgcggcgg ccaggtcaca tcacaatcca ttccactacc ggaggtctcg atgcagcttc
    22201 taaagggacg cgccctcgcc attctcttcc tgtctttcac gcttatgagc gcgcaggccg
    22261 cgccgcaggg cgaacgccaa ctcacgggct tcagccgcga tggggccgag gcgcagcgcg
    22321 cgctcgaagc gcgcttcgac gcgctgctca ggaaggagaa cctgcgcgag tggatgcggc
    22381 ggatggcggc gcggccgcat cacgtcggct cgccctacgg gaaggagaac gcggagttca
    22441 tggccgggtt gtttcgctcg tggggttacg aggtcgaggt cgagcggttc gacgtgttgt
    22501 tcccgacgcc gaaggtgcgc gtgttggaga tgacggcgcc tgaaagattt cgcgcgcgtc
    22561 tggaagagcc ggcgcttaag gaagatgcga cctcgaatca gaggtcggag cagttgccca
    22621 cctacaacgc ttactcggtg gacggcgacg tgacggggcc gctcgtctac gtcaactacg
    22681 gcgtgccgcg cgattacgag gagcttgagc gtcgcggcat cgacgtgaag ggtaagatcg
    22741 tcatcgcgcg ctacggcggc tcgtggcgcg gcatcaagcc gaaggtcgcc gccgagcacg
    22801 gcgcagtggg ctgtctcatc tactccgacc cgcgcgacga cggctacttt cagggcgacg
    22861 tctatccgcg cggcgcctac cgcaacgagc acggcgcgca acgcggctcc gtcgccgaca
    22921 tgccgctcta ccccggcgac ccgctcacgc ccggcgtcgg cgcgacgaaa gacgccaagc
    22981 gtctggagat taagggcgcg ccgacgctga ccaaaattcc cgtgctcccc atctcctacg
    23041 ccgacgcgca gccgctcctg cgctcgctcg ccggccccgt cgcgccgcag ggctggcgcg
    23101 gcgcgctccc cttgacctat cacctcggcc ccggccccgc ggtcgtccgc ctcaagctcg
    23161 aattcgactg gaagctcgtc ccggcctacg acgtgatcgc gcgtctgcgc gggagcgaac
    23221 gccccgacga gtggatcatc cgcggcaacc accacgacgc gtgggtcaac ggcgcggacg
    23281 acccgctgag cggcatggtt gcgctcatgg aggaggcgcg cgccgtgggc gagctggcga
    23341 agcagggcgc gcggccgaag cgcacgatca tctacgccgc gtgggacggc gaggagccgg
    23401 gcctgctcgg ctcgaccgag tgggtcgagg cgcacgccga ggagttgcgt cgccacgccg
    23461 ccctctacgt caacagcgac tcgaacggcc gcggcttcct cttcattggc ggctcgcaca
    23521 cgctcgaacg attcatcaac gaggtggcgc gcgatgtcac cgacccgcag aagaagattc
    23581 ctgtcttaga gcgcgcccgc gcgctacgta tcgacgacgg gagcgccgac gaccgccgcg
    23641 aggcacgcga gcgcgcagac ctccgtatca ccgccctcgg ctccggctcc gactacacgc
    23701 ccttcctcca acacctcggc gtcgccaccc tcaacctcgg cttcggcggc gaggacggcg
    23761 gcggctccta ccactcgatc tacgactcgt tcgaccatta cacgcgcttc ggcgacccca
    23821 acttcgacta cggcgtggcg ctcgcgcagg tgggcgggcg caccgtcctg cgcttcgcca
    23881 acgccgaagt gctcccgctc tccttcacac acctcgccga aacaatcggc cgctacgtcc
    23941 gcgaggtgac gaagctcgcc gacgacacgc gcgaagagat cgccgaaggc aaccgccgca
    24001 tcaccgacaa gaccttcgag gccgtcttcg accccactga agcctacgtc gcgccgaagc
    24061 cgaagccccc cgccccacac ctcaacttcg cgccgctcca gaacgcgctc gcccgcctcc
    24121 aggaaagctc gcgcaagtac cacgccgcgc tcggcgagcc ggccgcgcgc gaacgcctcg
    24181 cgcgcacgcc gcagacgcgc aacgaactca acgccgtgct catgcagatt gagcgcgcca
    24241 tgacgcgcga cgcggggctg ccgcgccgcc cctggttcaa gcaccagatt tacgcgcccg
    24301 gcttctacac cggctacggc gtcaagacac tgcccggcgt ccgcgaggcc gtcgaacagc
    24361 acgactggcg cgaggccgac gagatgatcc ttgtcgtcgc cggcacggtc gagcacctcg
    24421 ccgaaacaat cggccgctac gtccgcgaag tgacgaagct cgccgacgac acgcgcgaag
    24481 agatcgccga acgcaaccgc cgcatcaccg acaagacctt cgaggccgtc ttcgacccga
    24541 ctgaagccta cgtcgcgccg aagccgaagc cccccgcccc acacctcaac ttcgcgccgc
    24601 tccagaacgc gctcgcccgc ctccaggaaa gctcgcgcaa gtatcacgcc gcgctcggcg
    24661 agccggccac gcgcgaacgc ctcgcgcgcg cgccgcagac gcgcaacgaa ctcaacgccg
    24721 tgctcatgca gattgagcgc gccatgacgc gcgacgcggg gctgccgcgc cgcccctggt
    24781 tcaagcacca gatttacgcg cccggcttct acaccggcta cggcgtc
//
LOCUS       DASVES010000239        14097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_388650, whole genome shotgun sequence.
ACCESSION   DASVES010000239 DASVES010000000
VERSION     DASVES010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388650"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VF240_09860"
     CDS             <1..1012
                     /locus_tag="VF240_09860"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX7175551.1"
                     /translation="DDRYPAGRVTHTLGYPSDARTYGGGWIYGMQNRVLNIGYVTGLD
                     YTDPLLDPHAEFQRFKEHPFLSRLLEGGKMIRYGAKTIAAGGYFAVPKMYGDGFLLVG
                     DSAGFLNPQRLKGIHTAIKSGMLAAEAIFQGLLADDFSAKQLRSYKELVLDSWIMGEL
                     RKVRNFHAAFKHGRWLGLANSGLQFITGGRSWGMFDRASSEPGHLAMKKLKDFGYRGD
                     QLEQRYGKLRFDGKLTFNKVTDVYHAAVSHDEDQPVHLHVLDTEICSTRCAEEFGNPC
                     QRFCPAAVYEMVDDGADGRRRLQINFSNCVHCKTCDIMDPYQIINWVTPEGGGGPGYK
                     NM"
     gene            1099..1533
                     /locus_tag="VF240_09865"
     CDS             1099..1533
                     /locus_tag="VF240_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX7175552.1"
                     /translation="MRLSKAAHERLEKFLGEHLGEPELRLPGIHLHSGRIARWVTGVL
                     KIGAMTVGRHIFVAPRLLVRDEDGRLAAPGWLIAHEATHVVQFGKEGFAGFLYKYLRG
                     YFGALRQCGSWDAAARMTAYLAIAEECAAREAEHAYRKWNGN"
     gene            complement(1630..1875)
                     /gene="rpoZ"
                     /locus_tag="VF240_09870"
     CDS             complement(1630..1875)
                     /gene="rpoZ"
                     /locus_tag="VF240_09870"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7175553.1"
                     /translation="MAKAVESKSDLVGEETADVQPQEIDSKYRLIIVAAQRSKQLQRG
                     ARPRVDFDTARHKPTRIALEEVMRGYVTFETIEKEKD"
     gene            complement(1951..2637)
                     /gene="gmk"
                     /locus_tag="VF240_09875"
     CDS             complement(1951..2637)
                     /gene="gmk"
                     /locus_tag="VF240_09875"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7175554.1"
                     /translation="MLSESAPSESRGAAGEEADAHRGRLFIVSSPSGGGKGTLIRHVL
                     KSVPGISYSISWTTRARRAGEIDGVHYHFVSEDEFREIRERDGFLEWAIVHGNSYGTA
                     RSVVEQELGEGHDIILEIDVQGAESVRAATNDSVSVFILPPSFAVLRERLRMRGTDEP
                     ATVALRLRNARDEVRRYQEFDYVIINDDAQRAARQLAAVVYAERARRDRQEWIARRVL
                     ASFMESSPVE"
     gene            complement(2627..3499)
                     /locus_tag="VF240_09880"
     CDS             complement(2627..3499)
                     /locus_tag="VF240_09880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX7175555.1"
                     /translation="MTGFGRGTSNGDGYSVAVELKTVNNRFLDIHLRTGNELSGLEAT
                     IRRRIGERLTRGRVDVNINLDRTSEVSYEVNRPLVAGYIHAMRAMQEEFGLAGEMDIN
                     VLARLPGALQASRNGVGEEIAGAIEKALADALDDLQGMRESEGAALASEMRSLLDEIE
                     RNVPIIETLAGEQVVLYRSRVQKRIMELLARDGLEVVELDQGRLSQEVAYLADRSDIT
                     EEVARLRSHITQFRSIIDGGGEAGKRLDFLLQEFNREANTVLSKSTDLAIKESSLAIK
                     AAVEKLREQVQNVE"
     gene            complement(3599..5416)
                     /locus_tag="VF240_09885"
     CDS             complement(3599..5416)
                     /locus_tag="VF240_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7175556.1"
                     /translation="MRDLRRLLIYLKPHGGKFALATVAMFAVGLLQSAIGALIVPIFD
                     QALRPETDGGERTPTLFGLQNFIPDSGFEAWRTIALLLVVFTLAKGVAEYCSTYFMAR
                     VGQEAVLQLRRDMYAHLHAQSADFFARHRTNYLVSRLVGSTAAVETAVSHTLRDMLRE
                     GFTLIFFLTASFYYSWRLTLGSLLIAPVIGVLTARFGRSLRNLSRESYEGGQRLVDTA
                     QEALSNQTIVKAYVAERREHGRFFDVARQIMRANLRTAKISGFAPPTIEMIGVLAVAA
                     LLFFAQREITSGRMNAAQFVTFLFFLFSSYDPMRKLSRLHNGMEMALAAARHVWEVMD
                     ERAQIPEKSGSVELRPLREAIRLRDVSFGYPDDERSVLTNVSIDIKAGHIVALVGESG
                     GGKSTLTKLIPRFHDPVEGVVLWDGADLRDASVESLRRNVALVTQETVLFNDTVRYNI
                     GYGRPEATREEIEEAARVAYAHDFIKELPLGYDTIVGERGVFLSGGQRQRIAIARAVL
                     ADRTVLVLDEATSALDAESERLVQKALANLTRGRTTVVIAHRLSTVRRANLIVVMERG
                     RIVETGKHADLLARGGSYQRLYELQFAEEEETEAVGSEQ"
     gene            5704..6402
                     /locus_tag="VF240_09890"
     CDS             5704..6402
                     /locus_tag="VF240_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7175557.1"
                     /translation="MIADDDVSMRMLLAEYFRRLGFEVDDKESGEDALEPALSGAFDC
                     FIFDVTMPGISGLELLRRVRASGIDTPAMFLTAHDAVDDKVAGFQAGADDYLAKPFSP
                     RELEVRVESLLKRAGKWTQQTRKAEAAAADRLEIGALVIDKRRHEVSVGGERIDLTPL
                     EFHILEKLASEPGRAWSRNDLLDEVWSTDYEGYQRNVDPHINRLRRKIEADPKNPRYV
                     LTVRGVGYKLNEAP"
     gene            6399..8042
                     /locus_tag="VF240_09895"
     CDS             6399..8042
                     /locus_tag="VF240_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7175558.1"
                     /translation="MSYKNDLTALASVFAVALLACAPLSIASAQSTGKARRGGRTSAR
                     PQTQPATPDAQQPISTPVSLGGDSTTQLSSSVIIRWEGQPGVERYRLQVARDQGFEDI
                     TFDQAVTGRYHHVGVAPGNYFWRVAPAVVETGKYSTPLPVKVVPGPPVRNDRGVKVAT
                     GATGWRTATGQIPRPVTARLRAGGGYDFVGVNTDGTVYAIDGANGVAMWTARYQPGAR
                     RDEVVDPKGTLFGPLVVEGPAGRPDVVVAFAGGLRALRGATGREMWRAQFAGRAVAGT
                     IGDVNGDGKKEVVAVVTDPPSLIVLDAETGRDLSNSKLESDAVGAPAVLVREGKPLIV
                     VGLRGGVIEAWSASGERVNSEKLESDMTTGPLVVESPRALVVIVGGEKGLLALGASDL
                     KPMGMINTGDDSPRGTLTTADLDGDGSFEIVMVTRGGRVALIGTTDGKIKWVADGAGD
                     ADSATLADLNGDGTLDVIAAAGPVFAYGFSGKDGALIWKVEEEMAKGAASQPVDPRPR
                     SLTVAQSPKGEAILVGGDPARIGLRAVELPKGSVKADLR"
     gene            8059..8217
                     /locus_tag="VF240_09900"
     CDS             8059..8217
                     /locus_tag="VF240_09900"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX7175559.1"
                     /translation="MIFWLLALVLFVVWLVGVLFLGKGGFIHILLLHAVAVALVQFIH
                     DRRAARTQ"
     gene            8427..10028
                     /locus_tag="VF240_09905"
     CDS             8427..10028
                     /locus_tag="VF240_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7175560.1"
                     /translation="MRIIGSPTGLSKLTGSLLLLLLCAASAVAQPDSNRQTVAITYPL
                     DQSVSVKFRGTTRLPRLTGEAKVRRTGRRNTRVELEVKDLPRAFELGDVYTTFVLWAI
                     SPEGQADNLGEIKRGGSMFFNSKIDVTTPLQTFAMLVTAEPHFLVRTPSRMVVLENLP
                     PRNPGDAEVSTVSVQYIGNTSDYFNDTRVPDLADREFVRAPTSLHGARQAINLARFYG
                     AERDAAGELQEARTQLEAAENAWRLKQPEEEVDALARRAISLGVKSQEIAEARAAARK
                     RREEITRRDNAVREAEETAATAAEKIEELRAALRKEEHARELAERDALSANQQLRELR
                     SETARLREEVQTLRSDSESAKLKLARMEGERAAEDTRRANEVSAARQREAISALKQSL
                     ARFGSVRETERGLVLVLPDTLWSGARAADLTARASSSVVEPLAALLSNSPDLRIIIEA
                     HADNRGEAEAVLQLTQDRAEALAGRLVSAGVDSARVRAAGMGAANPVAPNTTPAGRAR
                     NRRIEIMLAPAAGSDAAESRAVNQN"
     gene            10175..12361
                     /locus_tag="VF240_09910"
     CDS             10175..12361
                     /locus_tag="VF240_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7175561.1"
                     /translation="MSPLEVIQLIGYGMAAALHLWMGALLVKRSPNLGRLERVLRSLA
                     LAVGTWHACNLILALHAMLGFDVLRWKTFLRVTNSVAVAAITLSYSLLLHVHLYLWAN
                     HRKRQLSPLERVRVYLSYLPAVFLTIAVWKLWQEPYGPMYEKLDFLLLPFALWAVYCL
                     LLVAATDFLIARSNESPSESRLLKTLAASFVGIAALIFAVYVFRLGQGETFLYLQTVA
                     NMGSLVPTALLAYQIYRYRYLELIIKESLVVATFAAVILVVYLYGIRTVGEWLTGRYG
                     LRAGLIESILILTLALVAAPLRSWLDRRFHRLFEHEVALYRDVVTSIGANAGRDQQLP
                     ELLRFVEERIAGGLGLRRVRILTEGREHPASDNDGKLIGDVEQVSDLNVGVGKRGYGI
                     GRDEVRVLEKDGAGELELGADDLRSSLNEQFRLMGFEPLEGIRQLRGAGFELAFALRR
                     EDRDVGVMLVDAPPHSLTRDVRAVLEILAGQVAIAIEDCRLVAENVQLERRLAHRERL
                     AALGQMAATVAHEIKNPLSAIKSIVQVMREDERLHNDYSRDLDLIVGETDRLSRSVTQ
                     MLSFARNAPHADMPRRLSEIVSSVVYLLQSETNARGARIHDDSAAVEAELDGATTGVV
                     RDAISNLLINALQATPRGGVISVEASIEDDTHARLTVTDKGPGVPDELRERIWEPFFT
                     TRQRGTGLGLAIVRKRIEEIGGSATLAAKRDGEGARFELDIPLRRL"
     gene            complement(12421..>14097)
                     /locus_tag="VF240_09915"
     CDS             complement(12421..>14097)
                     /locus_tag="VF240_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7175562.1"
                     /translation="LDTWFEIHIYPGSAGLSVYFHDIGARKQAEREMKVSEVRYRRLF
                     EAAHDGILILNAETGRITDVNPFLCELLNYPKAEFVGKELWEIGLFKDQEESGAAFRE
                     LQEKGYIRYEDLPLETKGGVRREVEFISNVYQENGHQVIQCNIRDITARKHLEAEREQ
                     LLIRARESQAAAEGANRLKDEFLATLSHELRTPLTAIVGWAGMLGNPQLDPAASLRAI
                     EVIRRNARIQVQMVDDLLDVSRIITGKLRLSVQPVDLGAIIIAAVDGLRPAAEAREIR
                     LQLQLDSPAGQVSGDPDRLQQVAWNLISNAIKFTPRGGRVLLRLERVESQVEITISDT
                     GKGISSEFLPHVFDRFRQADSTYTRAHGGLGLGLAIVRQLVELHGGTVRAESAGEGQG
                     ATFTVSLPLAAVRGATNKPDGVSQQALTSPELDCPPQLGGLRVLVVDDETETLEMLRF
                     MLEGCQAHVSTASSVAAALEAIKAEAFDVLITDIGMPEEDGYSLIAKVRALEKERGGR
                     IPAAALTAYASEEDRIRALRSGFQIHVPKPISPNELVAVVANLADRTGRG"
BASE COUNT         2550 a         4475 c         4540 g         2532 t
ORIGIN      
        1 tgacgaccgc tacccggccg gacgcgtcac gcacacgctc ggctatccat cggacgcgcg
       61 cacctatggc ggcgggtgga tttacgggat gcagaaccgc gtcctcaaca tcggctatgt
      121 caccggactc gactacaccg acccgctgct cgacccgcac gctgagttcc agaggttcaa
      181 ggagcacccg ttcctgagcc gcctgctcga aggcggcaag atgatccgct acggggcgaa
      241 gacaattgcg gccggcggct atttcgcggt gccgaagatg tacggcgacg gctttctttt
      301 ggtgggcgac agcgccggtt tcctcaaccc gcagcgactc aagggcatcc acacggccat
      361 caagagcggc atgctcgcgg ccgaggcgat ctttcaggga ctcctcgccg acgacttctc
      421 ggcgaagcag ctacgcagct acaaggagct ggtgctcgac agttggatca tgggcgagct
      481 acgcaaggtg aggaacttcc acgccgcctt caagcacggc cgctggctcg gactcgctaa
      541 ctcgggtctc cagttcatca ccggcgggcg ttcgtggggc atgttcgacc gcgcgagcag
      601 cgagccgggc catctggcga tgaagaagtt gaaggacttc ggataccgcg gcgaccagct
      661 cgaacagcgt tacggcaagc tgcgcttcga cggcaagctg accttcaaca aggtgacgga
      721 cgtttaccac gccgccgtct cacacgacga ggatcagccc gtacacctcc acgtgctcga
      781 cacggaaatc tgctcgacgc gctgcgccga agagtttggc aacccgtgcc agcgcttctg
      841 cccggcggcc gtctacgaga tggtcgatga cggggccgac ggccggcgac gcctccagat
      901 caacttctcc aactgcgtcc actgcaagac gtgcgacatc atggaccctt accagattat
      961 caactgggtc actcccgaag gcggcggcgg ccccggttat aagaatatgt aagtgggcgt
     1021 cgctgtcaca tcctgtgagt tcgcaccgga gacagcgggg gcgcaatctc ttcaacgtcg
     1081 cagcccactt cgtagcgaat gcgtctatca aaggcggcac acgagcgtct ggaaaaattc
     1141 ctgggcgagc acctggggga gccggagctg cgcctgccgg gcatccattt gcacagcgga
     1201 agaatcgcgc ggtgggtgac gggcgtgctc aagatcggcg ccatgaccgt cgggcgtcac
     1261 atcttcgtcg ccccgcgtct tctcgtccgc gatgaagatg gacgactcgc cgcccccggc
     1321 tggcttatcg cgcacgaggc gactcacgtc gtgcagttcg gcaaggaagg cttcgcgggg
     1381 tttttgtaca agtacctgcg cgggtatttc ggggcgctgc ggcagtgcgg gagttgggac
     1441 gcggcggcgc ggatgacggc ttacctcgcc atcgccgagg agtgcgcggc gcgggaagcc
     1501 gaacacgctt acaggaaatg gaacgggaat taaaaggttc ggacagcact cagacaaaaa
     1561 tgcgcgggcc aagccgcgtc tgccctgcgc ggcgcttgga gctatgtgag ggtaactttg
     1621 tggccgcgct taatcttttt ctttttcgat tgtctcgaag gtgacgtagc cgcgcatcac
     1681 ttcttcgagc gcgatgcgcg tcggcttgtg acgcgcggtg tcgaaatcga cgcgcgggcg
     1741 tgcgccgcgc tgtaattgtt tgctgcgctg cgcggctacg ataatcagac ggtacttgga
     1801 gtcgatctcc tgcggttgca cgtcggccgt ctcctcaccc accaagtcgc ttttactttc
     1861 cacagcttta gccattaatt ccttcttcct cgcccctgtt tgacaggtgg ccgtcaatca
     1921 aaccttatat ttataaaacg ttatgcccga ttattcaacc ggcgacgatt ccatgaagct
     1981 cgccagcacg cggcgagcta tccactcttg tctgtctcga cgcgcccgct cggcgtacac
     2041 gaccgcggct aattggcgtg ccgcgcgttg tgcgtcgtcg ttgatgatga cgtagtcgaa
     2101 ctcctgataa cgcctgactt cgtcgcgggc gtttctcagt cggagggcca cagtcgcggg
     2161 ttcgtccgtc ccgcgcatcc gcaaacgctc acgcaggacg gcgaaggagg ggggcaggat
     2221 gaagacgctt accgagtcgt tggtcgcggc ccgaaccgac tcggccccct gcacgtcgat
     2281 ctccagaatg atgtcgtgtc cctcgcccaa ctcctgctcg acgacgctgc gcgccgtgcc
     2341 gtaagagttg ccatgaacga tggcccactc aaggaatccg tcacgctcgc gtatctcgcg
     2401 aaactcgtcc tcgctgacga agtggtaatg aacgccgtca atctcgccgg cgcggcgtgc
     2461 gcgtgtagtc caggagattg agtagctgat gccggggacg cttttgagga cgtgccgaat
     2521 cagcgtgccc ttgccgccgc ccgacggcga gctgacgata aagaggcgtc cgcggtgagc
     2581 atcggcctct tcacccgccg cgccgcgcga ctcgctcggt gcggactcac tcaacattct
     2641 gcacctgctc gcgcagtttt tcgacggccg ccttgatcgc cagcgacgac tccttgatgg
     2701 ccagatcggt ggacttcgag agcaccgtat tggcctcgcg gttaaactcc tgtaggagaa
     2761 agtcgaggcg tttgccggcc tcgccgcccc cgtcgatgat ggagcgaaac tgcgtgatgt
     2821 ggctgcggag gcgggcgacc tcctcggtga tgtcgctgcg gtcggcgaga taagcgacct
     2881 cttgcgagag ccggccctga tcgagttcca cgacctcaag accgtcgcgc gcaagcagct
     2941 ccatgatccg cttctggacg cgggagcggt agagcacgac ctgttcacca gcgagcgttt
     3001 cgatgatggg gacgtttcgc tctatctcgt cgagcagcga gcgcatctcc gaggcgagcg
     3061 ccgcgccctc gctctcacgc atcccctgaa ggtcgtcgag cgcatcggcc agcgccttct
     3121 ctatcgcgcc ggctatctcc tcgcccacgc cgttgcgcga cgcctgaagc gcgccgggga
     3181 gccgcgccag cacgtttata tccatctcgc cagccagccc gaactcctcc tgcatggcgc
     3241 gcatcgcgtg gatgtagccg gcgacgaggg gccggttgac ctcgtaggag acttcggacg
     3301 tgcggtcgag attgatgttc acgtcaaccc ggccgcgcgt caggcgctcc ccgatgcggc
     3361 gacggatggt ggcctccagc ccggaaagct cgttgccggt gcggaggtgg atgtcgagga
     3421 agcgattgtt gacggtcttg agttcgacgg ccacgctgta gccgtcgccg ttggacgtgc
     3481 cccgtccgaa tcctgtcatt gatttcatat tggactatgt gtcttccggc agtaagcggc
     3541 gtgtgtcgag tgtcgggagc ggtgggccat gcggcgagtc gggcgccgcg cgtgcggctt
     3601 actgctcgct gcccaccgcc tcggtttctt cctcctcggc gaactgtaac tcgtagaggc
     3661 gctggtagga gccgccccgc gcgagcaagt ccgcgtgctt gccagtctcg acgatccgac
     3721 cgcgttccat gacgacgatc aggtttgcgc ggcggacggt cgagagccgg tgcgcgatga
     3781 cgacagtggt gcggccccgc gtgagattgg cgagcgcttt ctggacaagg cgctcggact
     3841 cggcgtcgag cgccgaagtg gcctcgtcta agacgagtac ggtgcggtcg gccaagacgg
     3901 cgcgggcgat ggcgatgcgc tgccgctgtc cgcctgaaag aaagactccg cgctcgccga
     3961 cgatggtgtc gtaaccaagg gggagttcct tgatgaagtc gtgcgcgtag gcgacgcgcg
     4021 cggcctcctc gatctcctca cgcgtggctt cggggcggcc gtaaccgatg ttgtatcgga
     4081 cagtgtcgtt gaacaggacg gtctcctgcg tgacgagcgc gacgttgcga cggagacttt
     4141 cgaccgaggc gtctcgcagg tcggcgccgt cccacaggac gacgccttcg acggggtcgt
     4201 ggaaacgtgg gatgagcttg gtcagcgtcg atttgccgcc gcccgactcg ccgacgagcg
     4261 cgacgatgtg gcccgccttg atgtcgatgg agacgtttgt cagaacactc cgctcatcgt
     4321 cggggtagcc gaaagaaacg tcgcgtaacc gaatggcttc gcgcaggggg cgcagctcga
     4381 cagagccgga cttctcggga atctgggcgc gctcatccat cacctcccac acgtggcgcg
     4441 cggcggcgag ggccatctcc atgccgttgt gcaggcgcga gagcttacgc atggggtcgt
     4501 agctggagaa gaggaagaag agaaaggtga cgaactgcgc ggcgttcatg cgccccgagg
     4561 tgatctcgcg ctgggcgaag aacagcagcg ccgccacggc gagcacgccg atcatctcga
     4621 tggtcggcgg ggcgaagccg ctgatcttag cggtgcgaag gttggcgcgc atgatttgcc
     4681 gcgccacgtc gaagaagcgg ccatgctccc gccgctcggc gacgtatgct ttgacgatgg
     4741 tctgattcga cagggcctcc tgcgccgtgt ccacgagccg ctgcccgccc tcgtatgatt
     4801 cgcgcgagag gtttcggagc gagcggccga agcgcgccgt caggacgccg atgacgggag
     4861 cgatgaggag cgagccgagc gtcagacgcc acgagtagta gaaggaagcg gtgaggaaga
     4921 agatgagtgt gaagccctcg cggagcatgt cgcgcaaggt gtgcgaaacg gccgtctcga
     4981 cagccgcagt cgagccgacg aggcgcgaga cgagatagtt cgtgcggtgg cgggcgaaga
     5041 agtctgccga ctgcgcgtgc aggtgcgcgt acatgtcgcg ccgcaactgg aggacggcct
     5101 cctgcccgac gcgcgccatg aagtaggtcg agcagtattc ggcgacgccc ttcgccaatg
     5161 tgaacactac gaggagcagg gcgattgtgc gccacgcctc gaagcccgag tcggggatga
     5221 agttttgcag tccgaagagg gtgggggtgc gctcgccgcc gtctgtctcg ggccggagcg
     5281 cttgatcgaa gatggggacg atgagcgccc cgatggcgct ctggagcaga ccgacggcga
     5341 acatcgcgac tgtcgccagg gcgaattttc cgccgtgcgg cttcagatag attaagagtc
     5401 gccggagatc gcgcatgctc tggtgcagtg actgtagtta accgtcggct gttgggagga
     5461 gttcggggcc gcatccggca tccggtaaag cagaactata cggacgggcc ggtttgtgcg
     5521 tcaagactcg cgcgttcgtg tgcgggaggc cgaaaaaacg ttgcggggcc gcacgcgggc
     5581 gtgtgtgatg ctgaggttga tgggttataa tctggccgcg aaacgaatgg ccgtggccgg
     5641 cgcgatttat caatcccaaa ttttatctgg atagaagatt aaaagagcgt gagtctccga
     5701 ctgttgatag cagatgacga cgtgagtatg cggatgctgc tcgcggaata tttccggcgt
     5761 ctcggcttcg aggtcgatga caaggagagc ggcgaagatg cgctggagcc ggcgctctcc
     5821 ggcgccttcg actgctttat cttcgacgtg acgatgcccg gaataagcgg cctcgaactc
     5881 ctccgccggg tgcgcgcgag cggcatcgac acgcccgcca tgtttctcac cgcgcacgac
     5941 gcggtggacg acaaagtggc cggctttcag gcgggcgccg acgactatct ggccaagccc
     6001 ttcagcccgc gcgaactcga agtgcgcgtc gagtcgctgc tcaagagggc ggggaagtgg
     6061 acgcagcaga cacggaaggc ggaggcggcg gccgccgacc gtctggagat cggcgcactc
     6121 gtcatcgata agcgccgcca cgaggtgtcg gtcggaggcg agcgcatcga cctgacgccg
     6181 ctcgaattcc acatcctcga aaaactcgcc agcgagccgg ggcgcgcgtg gtcgcgtaac
     6241 gatttgctcg acgaggtctg gagcacggac tacgagggtt atcagcgcaa cgtagacccg
     6301 cacatcaacc gcctgcgaag aaagatcgag gccgacccca agaacccccg gtacgttctc
     6361 accgtccgcg gggtcggcta taagctcaac gaggccccat gagttataaa aatgatctga
     6421 ccgccctcgc ctccgtcttc gccgtggcgc tcctcgcgtg cgccccgctg tcaatcgctt
     6481 ccgcacagtc caccggtaag gcccgaagag gcgggaggac gagcgcgcgg ccacagacac
     6541 agccggccac gccggacgcg cagcagccca tatcgactcc ggtttcactg ggcggcgact
     6601 ccaccacaca gttaagctcc tcggtcatca ttcgttggga ggggcagccc ggtgtcgaac
     6661 gctatcgcct acaggtcgcg cgcgaccaag gcttcgaaga catcaccttc gatcaggccg
     6721 tgacgggccg atatcatcac gtcggcgtcg cgcccggcaa ctacttctgg cgtgtggccc
     6781 ccgcggtggt cgaaactgga aagtattcga ccccgctgcc cgtgaaagtc gtgccggggc
     6841 cgcccgtgcg caacgataga ggcgtgaagg tggccacggg cgcgaccggg tggaggactg
     6901 cgacgggcca gataccccgt cccgttaccg cgcgtctgcg cgccggcggc gggtacgatt
     6961 tcgtcggcgt gaacacggac gggacggtct atgccatcga cggcgcgaac ggcgtcgcga
     7021 tgtggacggc gcgctaccag ccgggcgcgc gccgcgacga agtggtagac ccgaagggca
     7081 cgctgttcgg cccgctcgtc gtcgaaggcc ccgccggccg ccctgacgtg gtcgtcgcct
     7141 tcgccggggg gctgcgtgcg ctgaggggcg cgacgggaag ggaaatgtgg cgggcacaat
     7201 ttgcgggacg cgccgttgcc ggcacaatcg gcgacgttaa tggcgatggc aagaaagagg
     7261 ttgtcgcggt cgtcacagac ccgccgtcgc taatcgtcct tgatgccgag acgggccgcg
     7321 acctgtcgaa ctccaaactc gaaagcgacg ccgtcggcgc acccgcggtg ctggtacgag
     7381 aggggaagcc tttgatcgtc gtcggcttgc ggggcggcgt gatcgaggcg tggagcgcaa
     7441 gcggcgaacg cgtcaacagc gagaagttgg aatcggacat gacgacgggg ccgctcgtcg
     7501 tggagtcccc ccgcgctttg gtcgtcatcg tcggcggcga gaaggggttg ctggctctgg
     7561 gggcgtcgga tttgaagccc atgggcatga tcaacacggg cgacgactcg ccgcggggca
     7621 cgctgacgac ggccgacctc gacggagacg gatcgtttga gatcgtcatg gtgacgcgcg
     7681 gcggccgcgt cgcgctcatc ggcacgaccg acggcaagat caagtgggtc gccgatgggg
     7741 cgggtgacgc cgactcggcg acgctcgcgg acttaaacgg cgacggcacg ctcgacgtga
     7801 tcgccgccgc cggccccgtc ttcgcctacg gcttctcggg gaaggacggc gcgctcatct
     7861 ggaaggtgga ggaggagatg gcgaagggcg ccgccagcca gcccgttgac ccgcggccgc
     7921 gttcgctgac ggtcgcgcag tcaccgaagg gcgaggctat tctggtcggg ggcgacccgg
     7981 cgcgcatcgg actccgcgcg gtggagctgc cgaaaggctc ggtcaaggcc gacctgcgat
     8041 agcgggggcg cttcctcgat gatcttctgg ctgctcgcac tggttctttt cgtagtctgg
     8101 ctcgtcggcg tccttttttt aggtaagggc ggcttcatcc atatcctcct gcttcacgcc
     8161 gtcgcggtgg cgctcgtcca gttcatccac gaccgccggg ccgcgcggac gcagtgaaaa
     8221 atacaattct tctccccgtt gacaagcccg cgcgagggtt ttggtcctta ccggccgtga
     8281 agacaaattc ttcgtgcaaa cttttcggcc ggcaacagtg cgcgcccgag ttcgcatcaa
     8341 ttcattgacg aactgccaac ggcccgcttc cgggcacatc acaacgcgct acggcctctc
     8401 ggcccgaaga cgaggagaaa agctctatga gaataatcgg ctctcctacc ggactatcga
     8461 aacttacggg gtcgctgctc ctgctcctgc tctgcgcggc gagcgccgtc gcacagcccg
     8521 actcgaaccg tcagaccgtc gccatcacct acccgctcga ccagtcggtg agcgtcaagt
     8581 ttcgcgggac gacccgtctg ccgcgcctga ccggcgaggc gaaggtgagg cgcacgggcc
     8641 gccggaacac gcgcgtcgag ctggaggtga aagacctccc gcgcgccttc gagctgggag
     8701 acgtttatac gaccttcgtg ctgtgggcca tctcgccgga ggggcaggcc gacaacctcg
     8761 gcgagatcaa gaggggcggc agcatgttct tcaactcgaa gatcgacgtg acgaccccgc
     8821 tccagacctt cgccatgctc gtcaccgccg agccgcactt cctcgtccgc accccgagcc
     8881 ggatggtcgt cctcgaaaat cttccgccgc gcaaccccgg cgacgccgag gtctcgaccg
     8941 tcagcgttca atacatcggc aacaccagcg actacttcaa cgacacgcgc gtccccgacc
     9001 tcgccgaccg ggagtttgtc cgtgcgccca cctctctgca cggcgcgcgg caggcgatca
     9061 acctcgcccg cttctacgga gccgagcgcg acgccgccgg cgaacttcag gaggcgcgca
     9121 cgcagctcga agcggcggag aacgcctggc ggctcaagca gcccgaggag gaggtggacg
     9181 cgctcgcgcg ccgggccatc agcctcggcg tcaagtcgca ggagattgcg gaggcgcgcg
     9241 cggccgcacg caagcgccgt gaggaaatca cacgccgcga caacgcggtg cgcgaggcag
     9301 aggagaccgc cgccacggcc gccgaaaaaa tcgaggagct gcgcgcggcc ctgcgtaagg
     9361 aagagcacgc ccgcgagctt gccgagcgcg acgctctgag cgccaaccag caactacgcg
     9421 agctgcgctc ggagacggcc cgcctgcgcg aagaggtgca gacgctccgc tccgactcgg
     9481 agtcggcgaa gctcaagctc gcccgcatgg agggcgagcg cgccgccgag gacacgcgcc
     9541 gcgccaacga ggtaagcgcc gctcgtcagc gcgaagccat ctccgcgctc aagcaatcac
     9601 tcgcgcgctt cggttccgtc cgcgagacgg agcgcgggct ggtgctcgtt ctccccgaca
     9661 cgctttggtc gggcgcacgc gcggcggatt taacggcccg cgcgtcctcg tccgtggtcg
     9721 agcctctggc cgcccttctc tccaacagtc ccgacctgcg aatcatcatc gaggcacacg
     9781 ccgacaaccg cggcgaggcc gaggccgtgc ttcagctcac acaggacagg gccgaggcgc
     9841 tggccggaag actcgtctcc gcaggggttg acagcgcgcg cgtgcgtgcg gcgggcatgg
     9901 gcgcggccaa ccccgtcgcc ccgaacacca cgcccgccgg acgcgcgcgt aatcgccgca
     9961 tcgagatcat gctcgcgccc gccgccgggt ccgacgccgc ggagagccgc gccgtcaacc
    10021 agaattaact tccgcagccg gggcgactat ctgacgaggc gatagcctca tccgttacgc
    10081 gggagtcaac gccttcgggc gtcgcctcac ttcgtcgcat caacaaacaa gggcgggcgg
    10141 tggtaagatt ccccttaccg ccgcccgcgt tcttatgtcg ccgctcgaag tcatacaact
    10201 catcggctac ggcatggccg ccgcgctcca tctctggatg ggggcgcttc tggtcaagcg
    10261 cagccccaac ctcggccggc ttgagcgcgt gctgaggtcg ctggctctcg ccgtcggtac
    10321 gtggcacgcc tgcaacctca tcctcgccct tcacgcgatg ttgggcttcg acgtgctgcg
    10381 ttggaagacc ttcctgcggg tcacaaattc cgtggccgtc gccgccatca cactctccta
    10441 ctccctgctc ctgcacgtcc acctctatct ctgggcgaat cacagaaagc ggcagctcag
    10501 ccccctcgaa agggttcgcg tctatctctc gtacctgccg gcggtcttcc tgacgattgc
    10561 ggtctggaag ctctggcagg agccttacgg cccgatgtac gagaagctcg acttcctcct
    10621 cctgcccttc gcgctgtggg ccgtctactg tctcttactc gtggccgcca ccgacttcct
    10681 gattgcgcgc tcgaatgaat caccgagcga gagtcggctc cttaaaacac tcgccgcttc
    10741 attcgtcggc atcgccgcgc tgatcttcgc cgtctacgtc ttccgtctcg gtcagggcga
    10801 aacatttctc tacctccaga ccgtcgccaa catggggtcg ctcgtcccca cggcgctgct
    10861 cgcatatcaa atctatcgct accgttatct tgagctgatc atcaaggaga gtctggtcgt
    10921 cgcgaccttc gccgccgtca tcctcgtcgt ttacctctac ggcatccgca ccgtcggcga
    10981 atggctgacc gggcgttacg ggctacgcgc cggactgatc gagtcgatcc tcatactgac
    11041 gctggcgctc gtggccgcgc cgttgaggag ttggctcgac cggcgttttc accgcctctt
    11101 cgagcacgaa gtggcgctct accgcgacgt ggtgacaagc atcggcgcaa acgccgggcg
    11161 cgaccaacaa cttcccgaac ttctgcgctt cgtcgaggag cggatcgcgg gcggccttgg
    11221 tctgcgccgc gttcgtattc tcaccgaagg cagagagcac cccgcatccg ataacgacgg
    11281 gaagttgatt ggagacgtgg agcaggttag tgatttgaac gtcggtgtgg ggaagcgtgg
    11341 atatggaatc ggcagggacg aggtcagagt gttggaaaag gatggcgcgg gcgagttgga
    11401 acttggggct gatgatctgc gctcgtctct gaacgagcaa tttcgtctca tgggctttga
    11461 gccgttggaa ggaatcaggc aattacgggg ggcaggcttt gagctggcct tcgcgctgcg
    11521 gcgtgaggac agggacgtgg gcgtgatgtt ggttgacgcc ccgccgcact ctttgacgag
    11581 agacgtgcgc gccgtgcttg agattctcgc gggacaggtg gccatcgcca tcgaggattg
    11641 tcgcctcgtc gccgagaacg tccagcttga gagaaggctc gcccaccgtg aacgtctggc
    11701 cgcgctcggg cagatggccg caactgtcgc ccatgaaata aagaatccgc tgtcggcgat
    11761 aaaatccatc gtgcaggtga tgcgcgagga cgagcggctg cacaacgact actcacgcga
    11821 cctcgacctg atagtcggcg agaccgaccg cctgagccga agcgtcacac agatgctcag
    11881 cttcgctcgc aacgccccgc acgcggacat gccgcgccgt ctgagcgaga tagtcagttc
    11941 ggtggtttac ctgctccagt cggagacgaa cgcgcgcggc gcgcgcatcc atgacgactc
    12001 ggccgccgtc gaagccgaac tcgacggcgc gaccaccggc gttgtccgcg acgcgatttc
    12061 caacctcctc atcaacgccc tgcaagcaac gccgcggggg ggtgtcatta gcgtcgaagc
    12121 ctccatcgaa gatgacacgc acgcgcgcct gaccgtgacc gacaaaggcc ccggcgtgcc
    12181 cgacgaattg cgggagcgca tctgggagcc gttcttcacg acccgacaga ggggcacggg
    12241 cctcggactc gctatcgttc gcaagcgcat cgaggagatc ggcggcagcg cgacactggc
    12301 tgcgaaaaga gacggggaag gggcgcgctt cgagttggac atccctctgc gtaggctctg
    12361 agaacgcgcc gcgggaacta aattcggcgt caggtgtcag ttaaaaacaa aacatgaagc
    12421 ctagccccgc ccggtgcggt cggcgaggtt agcgacgacg gcgaccaact catttggact
    12481 gatcggcttc ggcacatgaa tctggaagcc tgagcggagc gcgcggatgc gatcttcttc
    12541 gctagcgtaa gccgtcagcg ccgccgccgg aattctgccg cctcgctcct tttctagtgc
    12601 tcgcacttta gcgatcaggg aatagccgtc ttcttccggc atcccgatgt cggtgattaa
    12661 aacgtcgaac gcttccgcct tgatcgcttc caacgcggcg gcaacagagc tggccgtact
    12721 cacatgcgcc tggcagcctt caagcatgaa gcgcagcatc tcaagcgttt ccgtttcatc
    12781 gtcaacgacc agcactcgca agcctcccag ttgcggcgga cagtcgagct ccggtgaggt
    12841 gagtgcctgc tgagacacgc catccggctt gtttgtcgca ccacgcaccg ccgcgagcgg
    12901 gagactgacg gtgaaagtcg cgccttgccc ttcgcccgcg ctttcagccc gcaccgtccc
    12961 tccatgcagc tcaactaact gacgaacaat cgccagcccc aatcccaaac cgccgtgagc
    13021 tcgcgtgtac gtggaatcgg cttgccggaa acggtcgaag acgtgcggca ggaactccga
    13081 cgagatgccc ttgccggtgt cgctaatggt aatctcgacc tgtgattcga cgcgttccag
    13141 acgtaacagc acgcgcccgc cacgcggtgt gaacttgatg gcgttcgaga tgagattcca
    13201 cgccacctgt tgcagccggt cggggtcgcc cgagacttgc cccgccggag aatcgagttg
    13261 caactggagg cgtatctccc tggcttccgc tgccgggcgc aggccgtcaa ccgccgcgat
    13321 gatgatggcg ccgaggtcaa cgggttgcac gctcaggcgc agcttgccgg tgataatgcg
    13381 cgagacatcg agcagatcgt caaccatctg cacttgtatc cgtgcattac gccggatgac
    13441 ttcaatggcg cgtaaggaag ctgccgggtc gagctgggga ttgcccaaca ttcccgccca
    13501 cccgacaatc gcggtcagcg gcgtgcgcag ctcgtgcgag agcgtggcga gaaattcatc
    13561 tttgagccgg ttcgctccct ccgccgccgc ctgtgactcc cgcgcgcgga ttaacagttg
    13621 ttcgcgctca gcctctaagt gtttgcgcgc ggtgatgtcg cgaatattgc attggatgac
    13681 ctgatggccg ttctcctgat agacgttact gatgaactcc acttcccggc gcacgccccc
    13741 cttagtttcg agcggtaaat cttcgtaacg gatgtagcct ttctcctgca actctcgaaa
    13801 cgcggccccg ctctcttcct gatccttgaa cagtccgatt tcccagagtt ctttccccac
    13861 aaactctgct ttcggatagt tcaacaactc gcacaggaat ggattcacgt cggtgattcg
    13921 ccccgtctcc gcattaagaa tgaggatgcc atcatgggct gcttcaaaga gccgccggta
    13981 acgaacttcc gaaactttca tttcgcgctc ggcctgctta cgcgcgccga tgtcatggaa
    14041 atagaccgac agtccagccg atcccggata aatatgaatc tcgaaccaag tgtcgag
//
LOCUS       DASVES010000240         4609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1410055, whole genome shotgun sequence.
ACCESSION   DASVES010000240 DASVES010000000
VERSION     DASVES010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410055"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VF240_09920"
     CDS             complement(<1..1918)
                     /locus_tag="VF240_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7175563.1"
                     /translation="MAAEVDRDLVSVQQARDLVEAAHRAQTQLAKFDQARIDRACEAM
                     AQVALREAARLGALAVEETGFGIPDDKREKNRFASEDVWRFFKGLRTVGVINETKDVM
                     EIASPRGVVAAIIPSTNPTSTAIFKILIATKSRNAVVLSPHPSAARCITETARVMREA
                     GVKEGLPAEAIGCITNSTIEGTEALMKHKLTAVILATGGIGLVRAAYSSGKPAFGVGP
                     GNVPVFVERTADVALAVQNILTGKCFDNGTICASEQSVVADAPVARQVREEFEAQGGH
                     FLSAAEADLLAKVVATPQRTLNPGIVGKSVDVIAKMAGISLPPGTRCMMVEVGGVGRE
                     HPLSMEKLSPILSFYVEDGVERGAARCFEVLSYGGMGHTAGIHTRSRDVARVYGTEMP
                     ASRVIVNSPTTHGAIGFSTALPPSMTLGCGSWGGNVTSDNISPWHLMDIKRVAFETRP
                     VPSKRPARSSSASTSAQPQPAMAQTPARPSVVASPAGIQAGAGGVSREEIAKIVDRFL
                     TGREGAQGAQTSGGGAATMRPTDSPAPVQTGIDIEPQRVSPSGGASPQPSAAPTAQTD
                     GGNGSQQGAPSQYNAPPPPAQRPAASSPPAENGGKQPAVSTNGKRAVDFVCEDDVRRA
                     MQKGEKIYIGARTII"
     gene            complement(2147..2413)
                     /locus_tag="VF240_09925"
                     /pseudo
     CDS             complement(2147..2413)
                     /locus_tag="VF240_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            2695..3546
                     /locus_tag="VF240_09930"
     CDS             2695..3546
                     /locus_tag="VF240_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7175564.1"
                     /translation="MNKPSDQNLEGGAREGGADGSLGEQLRRARLARGVSLREISDQT
                     RITMRHLEAIEADEYKHLPGGIFNRSFVRAFARCVGFDDKRALDLYARTARDHGADVD
                     EPLTSPHRSRIYTDGDTGRSPLLTFGLSALLVGILCLGIYALWYAYERRFGGVSTDGD
                     ESAQKSAAAQKGAQQSAPQSAPTPDPAAATVPDKLQVELRARDKSFWVSWRQDEDKKQ
                     KGRIISPGGPESMTLEKSINLRVGRGDVANLEVLVNGQPARLPSDAGVGDVEVLITKD
                     NFRQFLP"
     gene            3659..>4609
                     /locus_tag="VF240_09935"
     CDS             3659..>4609
                     /locus_tag="VF240_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7175565.1"
                     /translation="MITPDFPEALTFDDVLLVPARSDVLPAEVDTTTQFTRGISLRIP
                     LCSAAMDTVTESRLAIAIAQQGGIGVIHKNFPVERQAEEVDRVKRSESGMIVDPVTIR
                     HDRPVSDALVVMQTYKISGVPVVDEDGHLVGIITNRDLRFETRFDIPVSEVMTPQPLV
                     TVPVGTTLDEAKGVLQKHRIEKLLVIDGDKHLKGLITVKDIQKAIRYPHAAKDDLGRL
                     RVAAAIGATGDYQERAAELVRARVDCLVIDTAHGHSTRVVEAVKEIKRRHPETQLIAG
                     NVATGDATRELISAGVDAVKCGIGPGSICTTRVVTGAGVPQ"
BASE COUNT          757 a         1568 c         1510 g          774 t
ORIGIN      
        1 tgatgatggt gcgtgcgccg atgtaaatct tttcgccctt ctgcatggcg cggcgcacgt
       61 cgtcttcgca gacgaaatcg acggcgcgct tgccgtttgt cgagacggcc ggctgtttgc
      121 cgccgttctc ggcgggcggg gacgacgcgg cgggtctctg cgcgggcggc gggggcgcgt
      181 tgtactgcga cggcgcgccc tgttgcgagc cgttgccgcc gtcggtctgc gcggtgggcg
      241 cggccgaggg ttgcggtgag gcgccgcccg agggcgagac gcgttgcggc tcgatgtcga
      301 tgccggtctg gacgggcgcg ggcgagtcgg tgggccgcat cgtcgccgcg ccgcctcctg
      361 aagtctgtgc cccttgcgcg ccctcgcgcc cggtcaggaa gcggtcaact atcttcgcga
      421 tctcctcgcg gcttacgccg cccgcgccgg cctgaatgcc ggcgggagac gcaacgacgg
      481 acgggcgcgc cggcgtctgc gccatcgcgg gctgcggctg cgcggaagtg ctggcgctgc
      541 tgctgcgggc cgggcgcttc gaggggacgg ggcgcgtctc gaaggcgacg cgcttgatgt
      601 ccatcaggtg ccacggcgag atgttgtcgg aggtgacgtt gccgccccac gagccgcagc
      661 ccaaggtcat cgacggcggg agcgcggtcg agaagccgat agcgccgtgc gtggtcggcg
      721 agttgacgat gacgcgcgag gcgggcatct cggtgccgta aacgcgcgcc acgtcgcgcg
      781 agcgcgtgtg gatgccggcc gtgtggccca tgccgccgta ggataagacc tcgaagcaac
      841 gcgcggcgcc gcgctccacg ccgtcctcga cgtagaagga aagaatcggc gagagcttct
      901 ccatcgacag cgggtgctcg cgccccacgc cgcccacctc gaccatcatg cagcgcgtcc
      961 ccggcggcaa acttatgccc gccatcttcg cgatcacgtc aaccgacttg ccgacgatgc
     1021 ccggattcag agtccgctgc ggcgtggcga cgaccttcgc caaaagatcg gcctccgcgg
     1081 ccgaaagaaa atgtccgccc tgcgcctcga actcctcgcg cacctgccgg gcgacgggcg
     1141 cgtcggcgac gaccgactgc tcggaggcgc agatggtgcc gttgtcgaag cacttgccag
     1201 tgagaatgtt ttgtacggct agggccacgt cggccgtgcg ctcgacgaag acgggcacgt
     1261 tgccggggcc gacgccgaag gcgggcttgc ctgaagagta agccgcgcgc accagcccga
     1321 tgccgccggt ggcgaggatg acggccgtca gcttgtgctt catcagggct tcggtgccct
     1381 cgatggtcga gttcgtgatg cagccgatgg cctcggccgg caagccttct ttcacgcccg
     1441 cctcgcgcat gacgcgcgcc gtctccgtga tgcagcgcgc ggccgacggg tgcggcgaga
     1501 gcacgacggc gtttctcgac ttggtggcga tgaggatttt gaagatggcc gtggaggtcg
     1561 ggttggtcga tgggatgatg gcggcgacga cgccgcgcgg tgacgcgatc tccatcacgt
     1621 ccttcgtctc gttgatgacg ccgacggtac gcaagccctt gaagaaacgc cacacgtcct
     1681 cggaggcgaa gcggtttttc tcgcgcttgt cgtcggggat gccgaagccc gtctcctcga
     1741 cggccaacgc gcccaaccgc gccgcctcgc gcagcgccac ctgcgccatc gcctcgcacg
     1801 cgcggtcgat cctcgcctgg tcgaacttcg cgagctgtgt ctgcgcccgg tgcgccgcct
     1861 ccacgaggtc gcgcgcctgc tgcaccgaca ccaggtctct atcaacctcc gccgccatag
     1921 caacctccag gagccgtgat aagtgatgag tgataagtga taagaaaatc agtctttact
     1981 cttatcactc atcacttatc actcatcact ttgccctctc cttattgttc tcgcgcgagc
     2041 cggcggggag gttgcggccc tgctgatcgc cctcgccgag ctggccggca gagccgccgc
     2101 cctggagggc gcgctcgtcg gggctgaggc cggtgcggcc gttcatgggg agcacgcgct
     2161 cgacctcgcc gtgcgggcgc gggatgacgt ggacgctgat gagttcgcca acccttcggg
     2221 cggcggccgc cccggcgtcc gtggcggcct tcaccgcccc cacgtcgccg cgcacgaaga
     2281 tcgtcacgta gccggcgccg atatactcct tgccgagcag ttggacgttg gcggctttga
     2341 ccatcgcgtc ggccgcttcg accgctccga cgaacccttt ggtctcaacc attcccagtg
     2401 cttcgagagg cattgatgct aatcctcact tttctgttgt agaagttaaa agtagctttg
     2461 gcccaatctg aatttgttgc aaccgtagca ccgcgcgcgg caaagttcaa gagcgcgcat
     2521 ccttttgagc ggacgaagtt tgacacccct cgcggggcca cattatattg tggccctatc
     2581 gcactcgggc ggccgagtcc gggcggcctc cccatgtgga agcgggggag gcgacgggcg
     2641 gcgtcggccg gcacgggaga cacggggcga caatctcaaa caatcgcagg tcaagtgaac
     2701 aaaccctctg accagaatct cgaaggcggc gcgcgggagg ggggtgcgga cggctctctc
     2761 ggtgaacagt tgcgccgcgc cagactcgcc cgcggcgtct ccctgcgcga aatctccgac
     2821 cagacgcgca tcaccatgcg ccacctcgaa gccatcgagg cggacgaata caaacatctt
     2881 ccgggcggca tcttcaaccg cagcttcgtc cgcgcctttg cgcgctgcgt cgggttcgac
     2941 gacaagcgcg cgctcgacct ctacgcacgc accgcgcgcg accacggcgc cgacgtggac
     3001 gagccgctga cctccccgca tcgctcgcgc atctacaccg acggcgacac gggccgctcg
     3061 ccgctgctga ccttcggtct gagcgccctg ctcgtcggca tcctctgtct tggcatctac
     3121 gcgctctggt acgcctacga gcggcgcttc ggcggcgtct cgacggacgg cgacgagtcc
     3181 gcgcagaagt cggccgccgc tcagaagggc gcacagcagt cggccccgca gtctgcgccc
     3241 acgcccgacc cggccgccgc cacggttcct gataagttgc aagtcgagtt gcgggccaga
     3301 gacaaatcgt tctgggtgag ctggcgtcag gacgaagata agaagcagaa ggggcgcatc
     3361 atcagccccg gcggccccga gagcatgacg ctggaaaaga gcattaacct ccgcgtcggc
     3421 cgcggagacg tggcgaacct cgaagtgctc gtcaacggcc agccggcgcg tctgccgtct
     3481 gacgcgggcg tcggcgacgt ggaagtgctc atcacgaagg ataacttcag acagtttttg
     3541 ccctgaatgc gagttcaaag ttcaaggttc aaagttcaaa gtttgcccgc gaggtacggc
     3601 tctgaactgc gaaccttgct ctgtctttga actttgaact tggaactccg aggtaattat
     3661 gatcacgcct gactttccag aggccctgac cttcgacgac gtgctgctcg tgccggcgcg
     3721 ctccgacgtg ctgccggccg aggtggacac caccacgcag ttcacacgcg gcatcagcct
     3781 tcgcatcccc ctgtgctcgg cggcgatgga cacggtgacc gagagccggc tcgctatcgc
     3841 catcgcgcag cagggcggca tcggcgtcat tcacaaaaac ttccccgtcg agcggcaggc
     3901 cgaggaggtt gaccgcgtga agcgttcgga gtcgggcatg atcgttgacc ccgtcaccat
     3961 ccgccacgac cgccccgtct ccgacgccct cgtcgtgatg cagacctaca agatttccgg
     4021 cgtccccgtc gttgacgaag acggacacct cgtcggcatc atcaccaacc gcgacctgcg
     4081 cttcgagacg cgcttcgaca tccccgtctc cgaggtgatg acgccgcagc ccctcgtcac
     4141 cgtccccgtc ggcaccacgc tcgacgaggc gaagggcgtc ctccagaagc accgcatcga
     4201 aaagctcctc gtcatcgacg gcgacaaaca cctcaagggc ctcatcaccg tcaaggacat
     4261 ccagaaggcc atccgctacc cgcacgccgc caaagacgac ctcgggcgcc tccgtgtcgc
     4321 cgccgccatc ggcgccacgg gcgactatca ggaacgcgcc gccgagctgg tgcgcgcgcg
     4381 tgtcgattgt ctcgtcatcg acaccgccca cggccactcg acgcgcgtcg tcgaggccgt
     4441 caaagagatc aagcgccgtc accccgagac gcagctcatc gccggcaacg tcgccacggg
     4501 tgatgcgacg cgcgaactca tcagcgcggg cgtggacgcc gtcaagtgcg gcatcgggcc
     4561 ggggtcgatt tgcacgacgc gcgtcgtgac gggcgcgggc gtgccgcag
//
LOCUS       DASVES010000241         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_643847, whole genome shotgun sequence.
ACCESSION   DASVES010000241 DASVES010000000
VERSION     DASVES010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643847"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VF240_09940"
     CDS             <1..696
                     /locus_tag="VF240_09940"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7175566.1"
                     /translation="EMVTDEERDYLYNEYAKDRRMRLNVGIRRRLAPLLDNNRRQIEL
                     LNALLFSLPGSPFLYYGDEIGMGDNIYLGDRDGVRTPMQWSSDRNAGFSKADFERLYF
                     PVVNNPVYGFQSVNVETQMRYDTSLLHWMRLMIHLRKRHHVFGRGSMEFVKPANRKVF
                     AFTRHLEGETVLCVFNLARTAQPVELDLRRYDGCTPVEMLGDTPFPRVGELPYLLTLP
                     PWGFYWFLLQENV"
     gene            complement(808..1050)
                     /locus_tag="VF240_09945"
     CDS             complement(808..1050)
                     /locus_tag="VF240_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7175567.1"
                     /translation="MADCDRSLDLLSDLRDGVLADMDASWVKTHLDGCGDCNGVFLDL
                     EVIVVTARTLASENGLAYPDENGLWERIGIRRNIVH"
     gene            complement(1130..1708)
                     /locus_tag="VF240_09950"
     CDS             complement(1130..1708)
                     /locus_tag="VF240_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7175568.1"
                     /translation="MGAGAEVEVTGPSDFELAQKSAAGDSDAFEQLYRRHYRRVYALS
                     LRMLSDPTMAEDMTQEVFVNLFTKIGSFRGESQFTTWLHRMTVNQVLMYFRKASTRSE
                     LTTDEGETPVQIVRGTEDPGRMPVVDRIGLENAIRQLPLGYRTVFVLHDVEGYDHDEI
                     ARTLEISQGTSKSQLHKARLKLRGLIRQQSAG"
     gene            1990..>2192
                     /locus_tag="VF240_09955"
     CDS             1990..>2192
                     /locus_tag="VF240_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175569.1"
                     /translation="MPENNEQNFDAPDQQQPNEEKAVGAAAGAPFQTEAQEMKAQRGG
                     VQEDQIAGERAEGAHNQRRQNRNP"
BASE COUNT          405 a          724 c          668 g          395 t
ORIGIN      
        1 gagatggtca cggacgagga gcgcgactat ctctacaacg agtacgcgaa agaccgccgg
       61 atgcgcctca acgtcggcat ccgccgccgg ctcgcgccgc tcttagacaa caaccgccgc
      121 cagatagaac tcttaaacgc cttgctgttc tccttacccg gcagtccttt cctttactac
      181 ggcgatgaga tcggcatggg cgacaacatc tacctcggcg accgcgacgg ggtgcgcacg
      241 ccgatgcagt ggtcttcgga tcgcaacgcc gggttctcga aggccgactt cgagcggctg
      301 tacttccctg tcgtcaacaa cccggtctat ggcttccagt ccgtcaacgt cgagacgcag
      361 atgcgctacg acacgtcgct cctgcactgg atgcggctga tgattcatct tcgcaagcgt
      421 caccacgtct ttggccgcgg ctcgatggag ttcgtcaagc cggccaaccg caaggtcttc
      481 gccttcaccc gccacctcga aggcgagacc gtgctctgcg tcttcaacct cgcgcgcacc
      541 gcccagcccg tcgaactcga cctgcggcgc tacgacggct gtacccctgt cgagatgctc
      601 ggcgacacgc ctttcccacg cgtcggcgag ctgccttacc tgctgacgct cccgccgtgg
      661 ggcttctact ggtttttgct tcaggaaaat gtgtgaagaa aacggccccg gcgcggcagg
      721 cgcgcggggc cgtaaaatat tctctcgccg ccgccggcgc ggtcgggccg gcagggtgct
      781 ctttgagcgt gactgcttgg cgggtcttta gtggacgatg ttgcggcgga tgccgatgcg
      841 ctcccagagg ccgttctcgt cggggtaggc gagtccgttc tcactggcga gcgtgcgcgc
      901 cgtgacgacg atcacctcca ggtcgaggaa gacgccgtta caatcgccgc acccgtcgag
      961 gtgcgtcttg acccagctcg catccatgtc ggccaggact ccgtcgcgca agtcgctcag
     1021 gaggtcgagg ctccggtcac agtctgccat ttgttttgcc gctccaatcg cgttacggga
     1081 gtgtactcgc gcgccgcctt cgggggcgtg gcgagacctt tagaagcact caccccgccg
     1141 actgttgcct gatgaggccc cttaatttaa ggcgcgcctt gtggagttgc gacttgctcg
     1201 tgccctgtga gatttcgagc gtccgggcga tctcgtcgtg gtcgtagcct tcaacgtcgt
     1261 ggaggacgaa gacggtgcgg tagcctaagg ggagttgccg gatggcgttt tccaacccga
     1321 tgcggtcaac gacgggcatc cgtcccgggt cttccgtgcc gcgcacgatc tggacgggag
     1381 tctcgccctc gtcggtcgtc agctcggagc gcgtgctcgc cttacggaag tacatcagca
     1441 cctgattgac cgtcatccgg tgcagccacg tagtgaactg cgactcgccg cggaaactac
     1501 cgatcttggt gaagaggttg acgaagacct cctgcgtcat gtcctcggcc atcgtcgggt
     1561 cgctcagcat gcgtaaggag agagcgtaga cgcgccggta gtggcgccgg taaagctgct
     1621 cgaaggcgtc agagtcgccc gcggccgact tctgcgcgag ttcaaaatcc gacggccccg
     1681 taacctcgac ctctgctcct gcgcccactc ctcaccccct cacgccccaa cgggacgaat
     1741 aatgtctcac aagtcgcacg cccacgtctt tcaggatgcg ccctgacttt atcacgcctt
     1801 gcaccccgca cagaagcggc cgagtcggca gacattccgg caccggcctt gcttactcat
     1861 tcagcgaaac gcgacgaaag tcacggaaac ggcgcgccga acgtttgcga cgtgtccgct
     1921 cacacgtaca gacccggcgg cgcgcgtgtc cgctaactga acacgccaac gaaggaggaa
     1981 cgcaacgaga tgccagagaa caacgaacag aacttcgacg ctcccgacca gcaacaaccc
     2041 aacgaggaga aggccgtcgg cgcggcggcc ggggcgccgt tccagaccga ggcgcaggag
     2101 atgaaggcgc aacgcggcgg cgtgcaggag gatcagatcg ccggcgaacg ggccgagggc
     2161 gcgcataacc agcgccggca aaaccgcaac cc
//
LOCUS       DASVES010000242         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1456084, whole genome shotgun sequence.
ACCESSION   DASVES010000242 DASVES010000000
VERSION     DASVES010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456084"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..679)
                     /locus_tag="VF240_09960"
     CDS             complement(191..679)
                     /locus_tag="VF240_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7175570.1"
                     /translation="MRIGTEMPSLEGATEWFNATGARAAAEAKGQPTLVHFWSVSCGT
                     CKENMPRVQELRDARAVDGLRVIAVHMPRYEADTDAEAVRDAVTTYGITEPCAVDNQH
                     ALRDAFQNEQGYVPAYYLFDPEGKLRCFAAGENGLRMVLPAVERVLAAASRETAAKET
                     AA"
     gene            complement(775..1341)
                     /locus_tag="VF240_09965"
     CDS             complement(775..1341)
                     /locus_tag="VF240_09965"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7175571.1"
                     /translation="MDTTTTERRTARVGQPAPDFDMPSTKNMEKLNENVKLSDYAGKW
                     VVLLFYPLDFTFVCPTELTTFSEKIGDFEGIGAEIIGVSTDSVHSHRAWLRTPREQNG
                     VEGLKYPLASDITKQVSRDYGVLIEDKGIALRGLFVIDPEGVLRYAVVHDLNVGRSSE
                     ETLRVIQALQTGGLCQAEWRPGQETLKV"
     gene            complement(1433..1882)
                     /locus_tag="VF240_09970"
     CDS             complement(1433..1882)
                     /locus_tag="VF240_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX7175572.1"
                     /translation="MTTNSGDRGGVKGGDARQTLTRQRRVVLRVIEESREHLTAGEIY
                     EAARRELPRISHATVYNSLKFLKDGGFVREIGFGSGGASRFDRTGARHDHALCTRCGR
                     LVDFELPETAELLRAAARRSRFRAESIHLTLTGVCPDCRAAERAAPG"
     gene            2092..3096
                     /locus_tag="VF240_09975"
     CDS             2092..3096
                     /locus_tag="VF240_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX7175573.1"
                     /translation="MTLRNKLTIPRLALLLPLLVLLLSAPAEAKVTRYLTGNAGDVAP
                     VLAGPALDLGGGSTDVDAAIQWMIDQVRGCTTCATKVDVVILRSSGSNGYNDYIYAMN
                     GVDSVETLVITAARDSNTTAVETTVRNAEVIFFAGGDQCDYVKNFKGTRVETAVESVY
                     ARGGGVGGTSAGMAIQGEFTYDGCGGSATSSQALVNPYHRTVTFTYDFFHWADMGGTI
                     TDQHFVARDRMGRTMAFVARQIRDGRASSALGVAANEVTSIVVDKNGLARVMGNGPAY
                     FVLGDHFPESCEPGVPLTYSNFKIWKVASGGTFNLRTRPTTGFYTISVTGGALSGDPY
                     "
BASE COUNT          545 a         1056 c         1014 g          556 t
ORIGIN      
        1 atgagcgaca aaaaaagtcg aagccccgga ggggcgaaag aatcgcacgg ttgtgtcgcc
       61 gtcaccgtgc gattctttcg cccctccggg gctttctttt taattcggcg ctgacccacg
      121 gctgacgccg tgggctacat ccttccgccc ctgacggggc tttctgtcag tgtgtaaccc
      181 gcgcccgcgc ctacgcggcc gtctctttcg ccgccgtctc cctcgacgcc gcggcgagca
      241 cgcgctcgac cgccggcaaa accatcctca gcccgttctc gcccgccgcg aagcagcgca
      301 acttgccctc cgggtcgaag aggtagtagg cgggaacata cccctgctcg ttctgaaagg
      361 cgtcgcgcag cgcgtgctgg ttgtccacgg cgcacggctc cgtgatgccg taagtggtga
      421 cggcgtcgcg caccgcctcg gcgtccgtgt ccgcctcgta gcgcggcatg tggacggcga
      481 tgacgcgcag gccatcgacg gcgcgagcgt ccctcaattc ctgcacgcgc ggcatgttct
      541 ccttgcaggt gccgcagctc accgaccaga agtggacgag cgtgggctgc cccttcgcct
      601 cggcggcggc gcgcgccccc gtcgcattga accattcggt cgcgccctcc agcgagggca
      661 tctcggtccc gattctcatg ggcatatgtg aaacctcgtg atgagtgata agtgatgagt
      721 gatgagataa gaggtttttc ttatcactca tcactcatca cttatcacgg cttttcagac
      781 tttcaacgtc tcctgaccgg gccgccactc ggcctggcac agaccgccgg tctggagcgc
      841 ctgaatgacg cgcagcgtct cctccgagga gcggccgacg ttgaggtcgt ggacgacggc
      901 gtagcgcagg acgccttcgg ggtcgatgac gaagaggccg cgcagcgcga tgcccttgtc
      961 ctcgatgagc acgccgtagt cgcgcgagac ctgcttggtg atgtccgagg cgagcgggta
     1021 cttgaggccc tcgacgccgt tctgctcgcg cggcgtgcgc agccacgcgc ggtgcgaatg
     1081 caccgagtcg gtcgagacgc cgatgatctc ggcgccgatg ccctcgaagt caccgatctt
     1141 ttcgctgaag gtggtcagct cggtcgggca gacgaaggtg aagtcgagcg ggtagaacag
     1201 gagcacgacc cacttgcccg catagtcgga gagcttcacg ttttcgttca gcttctccat
     1261 gttcttcgta gacggcatgt cgaaatcggg ggcgggctgg ccgaccctcg ccgtccttct
     1321 ctcagtcgtt gtggtgtcca tcaaatcctc catgaagtat gtattgcgtg gaacaaaaaa
     1381 gtggggcggc gcggcacgtg agtcgtcacg cgccgcgccg ccccgcgaaa gtttatccgg
     1441 gcgcggcgcg ctcggcggcg cggcagtccg ggcagacgcc cgtgagcgtc aggtgaatcg
     1501 actcggcgcg gaagcgcgag cggcgggcgg cggcgcgcaa aagctcggcc gtctcgggca
     1561 gctcgaagtc aacgagccgg ccgcagcgcg tgcagagggc gtggtcgtgg cgcgcgcccg
     1621 tgcggtcgaa gcgactggcg ccgccgctgc cgaagccgat ctcgcgcacg aagccgccgt
     1681 ccttaaggaa tttcagggag ttgtagacgg tggcgtggct gatgcgggga agctcccgcc
     1741 gcgcggcctc gtaaatctcg ccggccgtca ggtgctcgcg gctctcctcg atgacccgga
     1801 gcacgacgcg ccgctggcgc gtcaatgtct gccgggcatc gccgcctttg acgccacccc
     1861 gatctccgct atttgtcgtc atgcttaaaa gtggaattag aataattcta aattggagat
     1921 ttgtcaatcg ccggccggca aaaaatcgcg ggcggcgacc gtcagggcgt ccccatcgcg
     1981 gcggccggga aaatttgtct tgctttcgcg cccgacgtga ggtagagaag ccgggcgcat
     2041 ttcgcgcatt tccacccacc agacaaacgt cagactaagg agaccactcg catgacccta
     2101 cgcaacaagc tcaccatacc ccgactcgcg ctcctcctgc ctctcctcgt gcttctgctg
     2161 tcggccccgg ccgaggcgaa ggtgacgcgc tatttgacgg gcaacgccgg cgacgttgcg
     2221 ccggtgctcg ccggccccgc cctcgacctc ggcggcggca gcaccgacgt ggacgccgcc
     2281 atccagtgga tgatcgatca ggtgcgcggc tgcaccacct gcgcgaccaa ggtggacgtg
     2341 gtcatcctcc gctcgtcggg gtcgaacggc tacaacgact acatctacgc gatgaacggc
     2401 gtggactcgg tcgagacgct cgtcatcacg gccgcgcgcg actcgaacac cacggccgtc
     2461 gagacgacgg tccgcaacgc cgaggtcatc ttctttgccg gcggcgacca gtgcgattac
     2521 gtgaagaact tcaagggcac gcgcgtcgag acggccgtcg agtcggtcta cgcgcgcggc
     2581 ggcggcgtgg gcggcacgag cgccgggatg gccattcagg gcgagttcac atacgacggc
     2641 tgcggcggga gcgccacgtc gagccaggcg ctcgtgaacc cctaccaccg caccgtcacc
     2701 ttcacctacg acttcttcca ctgggccgac atgggcggca ccatcaccga ccagcacttc
     2761 gtcgcgcgcg accgcatggg gcgcacgatg gcctttgtcg cgcggcagat acgcgacggc
     2821 cgcgcttcga gcgcgctcgg cgtcgccgcc aacgaagtca cttccatcgt cgtggacaaa
     2881 aacgggctgg cgcgcgtcat gggcaacggc cccgcctact tcgtcctcgg cgaccacttc
     2941 cccgaatcct gcgagccggg cgtcccgctc acttactcga acttcaaaat ctggaaggtc
     3001 gcgtcgggcg gcaccttcaa cctccgcacc cgccccacca caggcttcta caccatcagc
     3061 gtcacgggcg gcgccctgag cggcgacccg tactaaattg aagaggtcag aggttagagg
     3121 ttagaggtca gttaaaacag aaggccgggg ccggttgcga tgttgtcgca a
//
LOCUS       DASVES010000243        10671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_215433, whole genome shotgun sequence.
ACCESSION   DASVES010000243 DASVES010000000
VERSION     DASVES010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10671
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215433"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1300
                     /gene="preA"
                     /locus_tag="VF240_09980"
     CDS             29..1300
                     /gene="preA"
                     /locus_tag="VF240_09980"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEX7175574.1"
                     /translation="MADLSVNFAGIKSPNPFWLASAPPTNLGSMVERAFDAGWGGAVW
                     KTLGEPIRNVCSRLGSIDSGTQRMIGLNNIELITDRPLDVNLREIAECKKKYPKHALI
                     VSLMVESNREAWHEIAKRVEDTGADGVELNFGCPHGMSERGMGSAMGQVPEYTCMVTE
                     WVKEATRLPVIVKLTPNVTSINPPGRAAVRGGADALSLINTINSVVGVNLDTFVPHPN
                     VGGKAAHGGYCGPAVKPIALNMVSELARDPEVRIPISGIGGISTWRDAAEFLLLGATT
                     VQVCTAVMHYGYRIVEDMIDGLSNYLDEKGLPSVRDIIGASVERVTDWGNLDLNYKTV
                     ARIDHSLCIRCNLCHVACEDGAHQCIPLVQMNGARFPVVDEPECVGCNLCYLVCPSPG
                     CISMVRLDDGSQPLTWRELSSRPPETMTGDH"
     gene            complement(1482..3230)
                     /locus_tag="VF240_09985"
     CDS             complement(1482..3230)
                     /locus_tag="VF240_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEX7175575.1"
                     /translation="MRNYLPKAKSSLVLFAMLMVLACSSTAFAGGVTIVIVNIDGPGE
                     GFNDPTPVAPVGGNPETTRGAQRLFAFQHAATIWGGTLDSPVTVRIQAAFNPLGANVL
                     GSAGPTYLASDFGGVGLYPGSEFPLTWYFGTLADKRAGTELHPTPNFPDINCQFSSDF
                     NFYLGVDNNHGAQNDLVAVLLHEFGHGLNFANQVNESNGRNFGATNADPEGGLTDIFA
                     RHTFDNTTGLSWDQMTRAQRQASAINFGNVVWNGANVTAGAPSVLVLGSPEVRVLTPP
                     AIAGTKQFGTAAFGPAISSLVSPISATIVPAVDVDEDGGAAAFTTTDGCTAFSNAAAV
                     AGNIVLIERGGCGFAVKGRNASAAGAAGAIIYNQLANAAAAPPGMAGDGINDAFVTIP
                     TVSLTRADGLAIVGQPGATGFIGKDPNIRAGADPLGRVRLYMPNPVVGGSSGSHYDNI
                     AFRNLLMEPSINGDLTHNLKAPDDLTLELMRDVGWFPDFDLDGVATADDCDDSSDFSP
                     TVVIGSCNSGITNFMFTDGCTFMDKIQAIAASSSNHGIFVARVGQLANALKKQGTITA
                     AQKDALMSCAGSASIP"
     gene            complement(3243..3617)
                     /locus_tag="VF240_09990"
     CDS             complement(3243..3617)
                     /locus_tag="VF240_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175576.1"
                     /translation="MAVFVLLVGVWTMVSSQSAQQGKGGKKYVATEEIILDKASGKLR
                     KPTAEETQVMVDQVSSLTNRSSDGLAVNTQANGMKLVNLEGRFNGVVLGRANADGTTE
                     VRCVFTMEEAAEFLGLQEVPNQ"
     gene            4175..4426
                     /locus_tag="VF240_09995"
     CDS             4175..4426
                     /locus_tag="VF240_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175577.1"
                     /translation="MRRRKTPVARRLPPYPEFARFLVERGINSISLNPDTVIQTTHLI
                     HEAEAACAPPANGERTAAAAPLRHAAAHDAADVQRPVKN"
     gene            4655..6199
                     /locus_tag="VF240_10000"
     CDS             4655..6199
                     /locus_tag="VF240_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175578.1"
                     /translation="MKQRRHSFRVVLIESVLCAVVLCAVLWAVVPASAGRIDFNWSVC
                     VELADILEPERTWIPVGCEATLCCQGCPDAPIDWRIRVSGDPLDSVLLQFENLPPEAA
                     RKLVIKGKARWEGNALRVRPGETVVSGFKSDPRAVPAVATPRLFANKERLQRLQEAAE
                     REDAAPRGQAAPAAKPGAGRDLGRMEVVVEQLMGKYVVNEARVVYNVRRCRQGGANND
                     HFLLNNNIGSDNVVAILDARNGNNVCLEDEDYRGVGAIFVGDLRTSEQCTSETAIFSD
                     DNAMWFLTPTLWTSSLSDEKIANLLPILEAPVAVWLADPNPTPGTPERAEWEMATANH
                     YYNSQNAGIAFDATYTDVSANGDAVRLIGAGCSRVGQIKASSFYRPNQLNVYYVDNGP
                     TQSWRGKTCWQADPTSASHEPNIIFIDRRSRQETLAHEFGHSFSLDHPDEDGIMGINM
                     HNLMWSGGSMRTIISLGQAFRMNVNVTSMLNVNGVRTGFTRDPSCADTTTNPRCPHLA
                     LDVTPK"
     gene            6240..6761
                     /locus_tag="VF240_10005"
     CDS             6240..6761
                     /locus_tag="VF240_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175579.1"
                     /translation="MKSYVLLTTLLALCFAAPECRRHGAGTPSHPSPEQAAQARRAIV
                     NYFECEECEAGEIEAVVKLGQVAVPTLAATLLEGPSQANLELLRRHLTASYRELREYE
                     RTHPKAKVPGSEEQFVKTYMDNYVVLYQTRAATALGAIGGPEARRALEEASRKPLRGD
                     VQAAVKASLEKIK"
     gene            complement(6830..8020)
                     /locus_tag="VF240_10010"
     CDS             complement(6830..8020)
                     /locus_tag="VF240_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7175580.1"
                     /translation="MKGQPVEEFFDALRESLSGGAFVRLTLGKYRGDDPTLKNVYARR
                     VQIKGEDNLSLTFRHHTQDLVRNSTTDEALAIVAALLGHDFLSGHLFTTGFDLQLDFN
                     RKREPRLTRSRATFDTPPAAEHDRVKQRFVNAEGNVYLRALGVTDAEGRVRAGAGDKF
                     KQINKFVETVAHLLESAGLKGKQDLTLVDMGAGKGYLTFAVYDYLRNTLGLQRASVVG
                     VEARKELVELCNGVAREAGFEGLEFRQGLIRDYALGPVDVLVALHACDTATDEALHAG
                     LAAGAAVLITAPCCHKELRPQIETPEVLGGMLRHGIMLERQAEMLTDTLRALLLEASG
                     YQTRVFEFVTTEHTRKNTMIAAVRAGGKTDADAALRQFRELKEFYGVREQRLEKLLCA
                     AGDS"
     gene            complement(8191..9216)
                     /locus_tag="VF240_10015"
     CDS             complement(8191..9216)
                     /locus_tag="VF240_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7175581.1"
                     /translation="MKKGNRETLLLAAAGLGGLVAARALVRGRRGYELAGKTVLITGG
                     SRGLGLVMAREFAREGARVAICARDPEELERARADLVGRGADVYAVPCDVTDRDQVGE
                     MIDMVSRHFGEIDVLVNNAGVIQVGPVEVMTLEDYEQAMRVHFWGPLYTMLAVLPSMR
                     RRGGGRVVNISSIGGKIGVPHLVPYSASKFALVGLSEGMRAELRKDGVVVTTVCPGLM
                     RTGSPRNATFKGRHRDEYAWFAISDSLPVTSIGAERAARQIVAATRRGDAELIISTQA
                     FLAVKFHALFPEATADVLALVGRLLPGPGGIGKRRVKGAQSESALAPSVLTALSERAA
                     VKNNEVK"
     gene            complement(9241..10008)
                     /gene="xth"
                     /locus_tag="VF240_10020"
     CDS             complement(9241..10008)
                     /gene="xth"
                     /locus_tag="VF240_10020"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7175582.1"
                     /translation="MIIATWNVNSVLARLPLVLQWLGRARPDVLCLQETKCADERFPA
                     REFAELGYKSETHGQPTYNGVAILSRTPLTDVQRGFPNDEEGAHARLLAATVGGVRVV
                     NVYVPNGQSVGSEKFDFKLKWMRRLREFFDEECWPDDDVLLCGDFNVAPEDRDVHDPV
                     LWRGKILCSRPEREALQEVKDWGLADAFRQHNEGGGHYSWWDYRAGSFPRNTGLRIDH
                     VWLSESLAARCKRAWIDKEPRGWERPSDHTPVLVEIA"
     gene            10138..10518
                     /locus_tag="VF240_10025"
     CDS             10138..10518
                     /locus_tag="VF240_10025"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HEX7175583.1"
                     /translation="MRRVLIVEDDEVRCAWFRARLAGWEIDITCDVRQAVEWLSERDY
                     AAIFLDHDLVEEHYFSDAEDDERTGYAVAHWLARHPDSQRDATVLIHSLNYTGAGRML
                     DALHASGRDAEHVPFHYLRDELKF"
BASE COUNT         1897 a         3541 c         3379 g         1854 t
ORIGIN      
        1 tacgattcac tattcacgag gttttgaaat ggcagacctg agcgttaatt tcgcgggcat
       61 caaatcgccg aatcccttct ggctggcgtc ggccccgccg acgaatctgg ggtcgatggt
      121 cgagcgcgct ttcgacgccg gttggggcgg cgcggtgtgg aagactctgg gcgagccgat
      181 ccgcaacgtc tgctcgcggc tcggctccat cgactcgggc acgcagcgga tgatcggcct
      241 caacaacatc gagctgatta cggatcgccc gctcgacgtg aacctgcgcg agatcgcgga
      301 gtgcaagaag aagtatccga agcacgcgct catcgtctca ctgatggtcg aatcgaaccg
      361 cgaggcgtgg cacgagatcg ccaagagggt cgaggacacg ggggccgacg gcgtggagct
      421 taacttcggc tgcccgcacg gcatgagcga gcgcggcatg gggtcggcga tggggcaggt
      481 gcccgagtac acctgcatgg tcacggagtg ggtgaaggag gcgacgcggc ttcccgtcat
      541 cgtcaagctc acgccgaacg tgacgagcat caacccgccg gggcgcgcgg ccgtgcgcgg
      601 cggcgccgac gcgctctcgc tcatcaacac catcaactct gtggtgggcg tcaacctcga
      661 cacgttcgtg ccccacccga acgtcggggg caaggccgcc cacggcggct actgcggccc
      721 ggccgtcaag cccatcgcgc tcaacatggt ctcggagctg gcgcgcgacc ccgaggttcg
      781 catccccatc tcgggcatcg gcggcatctc gacgtggcgc gacgcggccg agtttttgct
      841 tcttggcgcg accaccgtcc aggtctgcac cgccgtcatg cactacggct accgcatcgt
      901 cgaagacatg atcgacggcc tctcgaacta cctcgacgag aagggcctgc cctcggtgcg
      961 cgacatcatc ggcgcgagcg tcgagcgcgt cacggactgg ggcaacctcg acctcaacta
     1021 caagacggtc gcgcgcatcg accactccct ctgcatccgc tgcaacctct gccacgtcgc
     1081 ctgcgaggac ggcgcgcacc agtgcatccc gctcgtccag atgaacggcg cgcgcttccc
     1141 cgtcgtcgat gagccggagt gcgtcggctg caacctctgc tatctcgtct gcccctcgcc
     1201 cggctgcatc tcgatggtga gactcgacga cggcagccaa cccctcacgt ggcgcgagct
     1261 ttcaagccgc ccgcccgaga cgatgacggg cgaccactaa accgcacttg tcggagcacg
     1321 cgcgcgcggg ccacttgaaa cacttctcgc ggaagcattc tttcgcccgc cggtcagatt
     1381 ccctccccgg cacgaaccag cgcacaaaaa aagggggcgg ctcgaatgag ccgcccccgt
     1441 caaaggagtc agagtttaag acagggccga agccccgccc gctagggaat gctggcgctg
     1501 ccggcgcagc tcatgagcgc gtccttctgg gcggcagtga tggtgccttg cttcttgagc
     1561 gcattcgcca actgcccgac cctggccacg aatatgccgt ggttgctgga gctggcggca
     1621 atcgcctgaa tcttgtccat gaaggtgcag ccgtcggtga acatgaagtt cgtgatgccg
     1681 ctgttgcagc taccgatgac gaccgtcggg ctgaagtcgg aggagtcgtc gcagtcgtcg
     1741 gccgtggcga cgccgtcgag gtcgaaatca gggaaccagc ccacgtccct catcaactcc
     1801 agcgtcaggt cgtccggcgc tttgaggttg tgcgtcaggt cgccattgat ggaaggctcc
     1861 atcaggaggt tcctgaaggc gatgttgtcg tagtgagagc cggacgagcc gccaaccaca
     1921 gggttcggca tgtacaggcg aactctgccg agaggatcag cgccggcccg gatgttgggg
     1981 tctttgccga tgaacccggt ggcgccgggt tgacccacga tggcgaggcc gtcggcgcgc
     2041 gtcaagctca ccgtcggaat ggtgacgaac gcgtcattga taccgtcgcc cgccattccc
     2101 ggaggcgctg ccgccgcgtt ggccaactgg ttgtagataa tcgcgcccgc ggctccggca
     2161 gccgacgcgt tccggccttt gacagcaaag ccgcagccac cgcgttcgat caagacgatg
     2221 ttgccggcga ccgccgcggc attgctaaac gccgtgcatc cgtcggtagt cgtaaaggcc
     2281 gctgccccgc catcttcgtc aacgtcaacc gccggcacga tggtggccga gatgggagaa
     2341 accagagagc tgatcgctgg gccgaaggca gccgtcccga actgtttggt tccggcgatg
     2401 gcgggcggcg tcaggactct cacttcagga ctgcccagaa cgagcacgct cggcgcgcct
     2461 gccgtcacgt tcgcgccgtt ccagacgacg ttcccgaagt tgattgccga agcctgacgc
     2521 tgggcacgag tcatttgatc ccagctcagg ccggtggtgt tgtcgaacgt gtggcgcgcg
     2581 aagatgtcgg ttaggccgcc ctcggggtct gcattagtcg ccccgaaatt tctgccgttc
     2641 gactcgttga cctggttagc gaaattcagg ccgtgaccga actcatgtag gagcaccgcg
     2701 acaagatcat tctgggctcc gtggttgttg tccactccca ggtagaagtt gaagtcgctg
     2761 ctgaactggc agttgatgtc ggggaagttg ggggtaggat gcaactcagt gccggcgcgc
     2821 ttatcggcca gcgtaccgaa ataccaagtg agcggaaact ccgaaccggg gtacagaccg
     2881 acaccgccga agtcagaggc caggtacgtc ggaccggcgc tccccagcac gttcgcgccg
     2941 agggggttga aggccgcctg gatgcggacg gtgacggggc tgtcgagggt gccgccccag
     3001 atggtggcgg cgtgctggaa ggcgaaaagg cgctgggcgc cgcgtgtggt ttccgggttg
     3061 ccgccgacgg gggccacggg cgtcgggtcg ttgaaaccct cgccggggcc gtctatgttg
     3121 acgatgacga tggtgactcc tccggcgaac gccgtgctgg agcaggcgag aaccatcaac
     3181 atcgcgaaaa ggacgagcga gctttttgct ttaggaaggt aatttcgcat ggtggttctc
     3241 ctttactggt tcggtacttc ctggaggccg aggaactccg cggcctcttc catcgtgaac
     3301 acgcaccgaa cctcggtcgt gccgtcggcg ttggcccggc ctaacaccac gccgttgaaa
     3361 cgcccttcca gatttaccag cttcatgccg ttcgcctgcg tgttgaccgc cagaccatcg
     3421 ctcgaacgat tcgtgagcga gctcacctga tcgaccatca cctgcgtctc ttctgcggtc
     3481 ggcttacgca gtttcccgct tgccttgtct aatatgatct cctccgtcgc cacgtatttc
     3541 ttcccgccct ttccttgttg tgcggactgc gaagagacca tcgtccacac tccgacgagt
     3601 agaacgaaca ctgccaatga ggcgaccaga cgggccggac tcggccgacc cttcgcgtgc
     3661 cttttaactt ggccgggggc tcgcaagtta ttcttcatgt gtttctcctt taaggaaccg
     3721 ttggacattt tcgaggttta caaatcgcga cgtgccctgt tgtaggagtc ttcgcgccgg
     3781 ttaaggaagg cggcaaagcc aattaaccgg ccgaagcctc cgcgttttga agtggcatgg
     3841 ccggcgggcc tggcggcccg agggccgtcc ggagacgcag acgcacgagt atggagagcg
     3901 agttcgatca aggggcggaa gatgcgcggc ccttcttagg tggcgaggga caacttgagc
     3961 ggcggcaacg ggcaaggatg ccagctcgaa gcgcagacgt tatgtgttta ggctttttac
     4021 ctgaagcggt gaatcaatca ccttcggcga aaggccagca acggattatg ggagcggagc
     4081 ttattccaag accggcgtct tgtcaaccga attgaggcgg ccgcctcggg acggcgccac
     4141 ccggcgcccc cggcccgccg gtgacgcggc gcgcttgcgc cggcgcaaaa ccccggtcgc
     4201 gaggagactt ccgccgtacc ccgagttcgc ccgcttcctc gtcgagcgcg gcatcaactc
     4261 catctcgctc aaccccgaca ccgtcatcca gaccacccac ctcatccacg aggccgaggc
     4321 cgcgtgcgcg ccgccggcca acggcgaacg cacggccgcc gccgcccctc ttcgtcacgc
     4381 cgccgcgcac gacgccgcag acgttcagcg tcctgtaaag aattaagcgg cggcgagtag
     4441 tggctccgct tgataaacca gcttccggct ccctctctgc gcgcgctacc cgatgaaatg
     4501 ttttaccccg gaatggcatt aacattttag agagctgtag gccggacaat tatctccaga
     4561 tgagttaacg ggagagagag tcctcgtcgg atttgaaatt gactcactac ccggcggcgg
     4621 cggttcggcc gggccacacg taaaggagcg agtcatgaag caacgaaggc atagcttccg
     4681 tgttgtgttg attgagtccg tcctctgcgc cgtcgtcctc tgcgccgtcc tctgggccgt
     4741 cgtccccgcc tcggccgggc gcatcgactt caactggagc gtctgtgtcg agctcgcaga
     4801 tatactcgaa ccggagagga cgtggattcc ggtcggctgc gaagccacgc tctgctgtca
     4861 gggatgcccc gacgcgccca tcgactggcg catccgcgtc tctggcgacc cgctcgacag
     4921 cgtcctcctc cagttcgaga acctcccgcc agaagcggcg cggaaactcg tcatcaaagg
     4981 caaggcccgc tgggaaggta atgccctccg cgtcaggccg ggcgaaaccg tcgtcagcgg
     5041 tttcaagtcc gacccccgcg ccgtgccggc cgtcgcgact ccgcgcctgt tcgccaataa
     5101 agagcgcctc cagcgtcttc aggaggccgc ggagcgcgag gacgccgcgc ctcgcgggca
     5161 ggccgcgccg gccgcgaagc ccggcgccgg ccgcgacctc gggcggatgg aagtcgtcgt
     5221 cgagcagttg atggggaagt acgtggtcaa cgaggcccgc gtggtctaca acgtcaggag
     5281 gtgccgacag gggggggcca ataatgacca cttcctcctc aataacaaca tcggcagtga
     5341 caacgtcgtg gcgatcctcg acgcgcgcaa tgggaacaac gtctgtctcg aagatgagga
     5401 ttatcgtggc gtcggagcca tcttcgtggg cgacctccgc accagcgagc agtgtacctc
     5461 cgagacggcc atcttctccg acgacaacgc gatgtggttc ctcacgccga ccctctggac
     5521 cagctcgcta tccgacgaga aaatcgcgaa cctcctgccg atcctcgaag cgcccgtagc
     5581 cgtctggctc gccgacccca accccacgcc cggcacgccg gagcgcgccg agtgggagat
     5641 ggccaccgcc aaccactact acaactccca aaacgccggc atcgcgttcg acgcgaccta
     5701 cacggacgtt tccgcaaacg gcgacgccgt gcggctcatc ggcgcgggct gcagcagggt
     5761 aggccaaatc aaggccagca gcttctacag gcccaaccag ctaaacgtct actacgtaga
     5821 caacggcccc actcagagct ggaggggcaa gacctgctgg caggccgacc ccaccagcgc
     5881 gtcccacgaa cccaacatca tcttcatcga ccggagatcc cgccaggaga ctctcgcgca
     5941 cgagttcggc cactcgttct ctctggatca ccctgacgag gatgggatca tgggcatcaa
     6001 catgcacaac ctcatgtgga gcggcggctc catgcgcaca atcatctccc tcgggcaagc
     6061 cttccgcatg aacgtcaacg tcacatccat gctcaacgtt aacggcgtac gcaccggctt
     6121 tacgcgcgat ccctcctgcg ccgacacgac gaccaatcct cgctgtccgc atctggcgct
     6181 ggacgtgact cccaagtgag ggccgggccg ccccgacgcg gccgaaacgg aggacgaaca
     6241 tgaaaagcta tgtactgctc acgaccctgc tggccctctg cttcgccgcc cccgaatgcc
     6301 ggaggcacgg cgcggggacg ccctcacacc cgtcgccgga acaggcggcg caggcccgcc
     6361 gcgccatcgt caattacttc gaatgcgagg agtgtgaggc gggagagatc gaagccgtcg
     6421 tcaagctggg ccaggtggcc gtgcccaccc tcgcggcgac gctcctcgaa ggcccctcgc
     6481 aagccaacct cgaactactc cgccgccacc tcaccgcctc ttaccgcgag ctgagggagt
     6541 acgagcggac gcaccctaaa gcgaaggtgc cggggagcga ggagcagttc gtcaaaactt
     6601 acatggacaa ttacgtcgtc ctctaccaga cgagggcggc gacggcgctc ggggccatcg
     6661 gcggcccgga ggcccggcgg gcgctggagg aggcttcgcg caagccgctg cgcggggacg
     6721 tgcaggccgc ggtcaaggcg tctctggaga agataaagta gcccgagagg ctggcgccgg
     6781 gcggggcgcg cagacggggg ccggaggctc acggcccgcg cccccgtcgt tagctgtcgc
     6841 cggccgcgca caggagtttt tcgaggcgct gctcgcggac gccgtagaac tctttgagtt
     6901 cgcggaactg tcggagggcg gcgtccgcgt ccgtcttccc tccggcgcgg acggcggcga
     6961 tcatcgtgtt cttgcgcgtg tgctcggtgg tgacaaactc gaagacgcgc gtctggtagc
     7021 cggaggcttc gagcagtaac gcgcgtagcg tgtccgtcaa catctccgcc tgacgctcca
     7081 gcatgatccc gtgcctcagc attccgccca gcacctccgg cgtctcgatc tgcggccgca
     7141 gctctttgtg acagcacggc gcggtgatga gaacggcggc cccggcggcc agccccgcgt
     7201 ggagcgcctc gtcggtcgcc gtgtcgcagg cgtgcagggc gacgaggaca tcgacggggc
     7261 cgagcgcgta atcgcgtatt aatccctgtc ggaactccag cccctcgaac cccgcctcgc
     7321 gcgccacgcc gttgcacaac tcgaccaact ctttccgcgc ctccacgcca acgaccgacg
     7381 cgcgctgtag tcccagagtg ttccgcagat agtcgtagac ggcgaaggtg aggtagccct
     7441 tgcccgcgcc catgtccacg agcgtgaggt cttgcttacc tttcagcccc gcgctttcca
     7501 gaaggtgcgc caccgtctcg acgaatttgt ttatctgttt gaacttgtcg cccgcgcccg
     7561 cgcgcacgcg cccctccgcg tccgtcacgc cgagcgcgcg cagatagacg ttgccttccg
     7621 cgttgacgaa tcgctgcttg acgcggtcgt gttcggcggc gggcggcgtg tcgaaagtcg
     7681 cgcggctgcg tgtgagccgc ggctcgcgtt tgcggttgaa gtcgagctgc aagtcgaacc
     7741 cggtcgtgaa gaggtggccg ctcaggaagt cgtgcccgag gagcgcggcg acgatggcga
     7801 gagcttcatc ggtcgtggag tttcggacga ggtcttgagt gtggtggcgg aaggtgagcg
     7861 agaggttgtc ctcgcccttg atctgcacac gtctggcgta gacgtttttc agcgtggggt
     7921 cgtcgccgcg gtatttgccg agagtcagcc tgacgaacgc gccgccggac aggctttcgc
     7981 ggagtgcgtc gaagaactct tcaaccggct ggcccttcat ggatcaccgc tttcctgaat
     8041 caaaacatgc atcgttgaga caagtaagag aacacggatg agacggacgt gacgaaccga
     8101 cacggattga tccgtgccga tccctctcat ccgtctcatc cgtgttcact cgacctgtcc
     8161 cgactggatg cgcttcgatt tagaccgccg tcacttcacc tcgttgttct ttaccgccgc
     8221 tcgttcgctg agtgcagtca aaacagaagg cgccagcgcc gactcgctct gcgcgccctt
     8281 cacgcggcgc ttgccgatgc cgccggggcc gggcagaaga cgacccacga gcgccagcac
     8341 gtcggccgtc gcctcgggga agagcgcgtg gaatttgacg gccaagaagg cctgcgtcga
     8401 gatgatgagt tcggcgtcgc cgcgtcgtgt ggccgcgacg atctggcgcg cggcgcgctc
     8461 ggccccgatg gacgtgacgg gcagggagtc gctgatggcg aaccacgcgt actcgtcgcg
     8521 gtgccggccc ttgaaggtcg cgttgcgcgg gctgcccgtc cgcatcagcc ccggacagac
     8581 ggtcgtcacc acgacgccgt ccttccgaag ctccgcgcgc atcccttcgg agagaccgac
     8641 gagcgcgaac ttgctcgcgc tgtaggggac gagatgcggc acgccgatct ttccaccgat
     8701 ggaggagatg ttgacgacgc gcccgccgcc tcgccttcgc atcgacggaa gcacggcgag
     8761 catcgtgtag agcggccccc agaagtggac gcgcatcgcc tgctcgtaat cttcgagcgt
     8821 catcacctcg acggggccga cctgaatgac gcccgcgttg ttgaccagca cgtcgatctc
     8881 gccgaagtgg cgcgagacca tgtcgatcat ctcgcccacc tgatcgcggt cggtcacgtc
     8941 gcacgggacg gcgtacacat ccgcgccgcg gcccacgagg tcggcccgcg cgcgctcaag
     9001 ctcttcgggg tcgcgcgcac agatggcgac gcgcgctccc tcgcgcgcga actcccgcgc
     9061 catcaccagc cccagtccgc gcgagccgcc cgtaatcaac acggtcttgc ctgccagctc
     9121 atacccgcgc cggccgcgca cgagcgcgcg cgccgcgacc agcccgccca gacccgccgc
     9181 cgccagcaac agcgtctcac gattcccctt cttcattccc ttctcccttc acagatggct
     9241 tcaggcgatc tcgaccaaca cgggcgtgtg gtcggagggt cgctcccagc cgcgcggctc
     9301 tttgtctatc caagcccgct tgcagcgcgc ggccagcgac tcggagagcc agacgtggtc
     9361 gatgcgcagg cccgtgttgc gtgggaagga gccggcgcgg tagtcccacc acgagtaatg
     9421 gcccccccct tcgttgtgct gccggaaggc gtccgcgagg ccccagtctt tgacctcctg
     9481 aagggcttcg cgctcggggc ggctacagag aattttgccg cgccacaaca cggggtcgtg
     9541 tacgtcgcga tcctccggcg cgacgttgaa gtcgccgcac agcagcacgt cgtcgtccgg
     9601 ccagcactcc tcgtcgaaga actcgcgcag gcggcgcatc cacttgagct tgaagtcgaa
     9661 cttctcggag ccgaccgact gcccgttcgg gacgtagacg ttgacgacgc gcacgccccc
     9721 gaccgtcgcc gccagcagac gcgcgtgcgc cccctcctca tcattgggga acccacgctg
     9781 cacgtccgtc agaggagtgc gcgagagtat cgccacgccg ttgtatgtgg gctggccgtg
     9841 cgtttcagac ttgtagccga gttcggcaaa ctcgcgggcc gggaagcgct cgtcggcgca
     9901 cttcgtctct tgcaggcaga gcacgtcggg gcgcgcccgg ccgagccatt gcaagaccag
     9961 gggcaggcgc gccagcaccg agttcacgtt ccacgtggcg atgatcattt cgccgacaat
    10021 cttataacgt gacggcgcgt ttttgtaggc ggcgcgcccc gcgcgttgta acctcaagac
    10081 taaagtgaca aattaatgac ggcgcgcggc ccccacaggg cgcggccaca aacttttatg
    10141 agacgagtgc tgatcgtcga ggacgatgag gtgcgttgcg cgtggttccg cgcacggctc
    10201 gcgggctggg agatcgacat cacgtgcgac gtgcggcagg ccgtcgagtg gctttcggag
    10261 cgggattacg cagccatctt cctcgaccac gacctcgtcg aggagcatta cttctccgac
    10321 gccgaggacg acgagcggac gggctatgcc gtcgcccact ggctcgcccg acaccccgac
    10381 agccagcgcg acgcgaccgt cctcatccac tcgctcaact acaccggggc ggggcgcatg
    10441 ctcgacgcgc tccacgcctc gggccgcgac gccgagcacg tgccgtttca ttatttgcgc
    10501 gacgagttga agttttaagg ggccaccgcg atttttgttg agtgtacggc aggtaggatt
    10561 caccgcggag gcgcagagga cgcagagact acgcagaggc ttgacgctta cacccgacgt
    10621 tgagtaaatc cgtcaagcct gcggcgcccc ctctgcgtcc tttgcgcctc c
//
LOCUS       DASVES010000244         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_617538, whole genome shotgun sequence.
ACCESSION   DASVES010000244 DASVES010000000
VERSION     DASVES010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617538"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /gene="ccsA"
                     /locus_tag="VF240_10030"
     CDS             <1..483
                     /gene="ccsA"
                     /locus_tag="VF240_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7175584.1"
                     /translation="APAQLEAVAAQFVSGRGADLALGVRGNPVELSALITAAAVTLFV
                     IVFSFRTERLRSALPSLEKIDSLMYKTAGITFAGLALLLITGAVWANESWGRYWGWDP
                     KEVGALVAWLTYGGFLHVRISRGWTGRPAAYFALVAFLLILFTYLGVSYLLPGMHSYA
                     "
     gene            575..1912
                     /gene="hemA"
                     /locus_tag="VF240_10035"
     CDS             575..1912
                     /gene="hemA"
                     /locus_tag="VF240_10035"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX7175585.1"
                     /translation="MTLVLTGVSHKTAPVEVRERLAFAKEALPGYLSALVDGRAVLEG
                     MILSTCNRVELLVDVDPAHFEEGRSRVADFLASRGVGLGEDLGRHVYEYAGEEAARHL
                     LRVASSLDSMVLGEPQILGQVRQAYADACAAGTAGRTLHRLLHHAFHAAKRVRTETNI
                     ASAAVSVSYAAVELGRKILGTLEGRTVLLVGAGEMAELAARHLAKAGAARILVANRTH
                     ETAHRLASEFGGEAVPYESFPSFLAEADICICSTGARKYLVTAQMARVALELRGNRPS
                     FFIDISVPRNVDPRVGEVANLFVFDVDDLESVVASNLCERAREAKRAEQIVEAETAQF
                     HKAMRALEIGPVIASLKGKMRRIAEEEFRRQRARLGDLTPEQERAVEQMLAATVNRIS
                     HPLIERLRRSFDTGDAGSVGAWRVSFGLEEELEIVSSVKTHLAVAFKLQAVTT"
     gene            1909..2862
                     /gene="hemC"
                     /locus_tag="VF240_10040"
     CDS             1909..2862
                     /gene="hemC"
                     /locus_tag="VF240_10040"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7175586.1"
                     /translation="MRDELIIGSRGSKLALWQAEWVRARLAALSPRLDVRVEVVSTSG
                     DVLKDAPLSVIGGQGAFTKELEHALLERRIDLAVHSLKDLPTVIPDRLTLAATPARED
                     ARDALVLPAGTSGGGVVSVLDLPAGARVGTSSLRRRAQLRHLRPDLVLNDLRGNVDTR
                     LRKLDAGEFDAIVLAAAGLRRLGLAGRISAHVSPDELLPAVGQGALGLEARADDEETL
                     SLVARLDHAPTRAACEAERALLRQFGAGCQVPIAAHATVEGLFLKLEGLVAAPSGEDV
                     LKASVVGFMGDAEGIGEELAHRLMARGAAFLLAGAATALAS"
     gene            2955..>3142
                     /locus_tag="VF240_10045"
     CDS             2955..>3142
                     /locus_tag="VF240_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175587.1"
                     /translation="MKKENILYCIIGALLGFIVGFGFANTTNQRGYVAQAAAAAGGQP
                     GQMAGLPPNHPPIEGAAAA"
BASE COUNT          485 a         1080 c         1050 g          527 t
ORIGIN      
        1 gcgccggcgc agctcgaagc ggtggcggcg cagttcgtca gcggacgcgg cgccgacctc
       61 gcgctcggcg tgcgcggcaa ccccgtcgag ctgtcggcgc tcatcacggc cgccgctgtg
      121 accctcttcg tcatcgtctt cagcttccgc actgagcgtt tgcgctcggc gttgccctcg
      181 ctggagaaga tcgactcgct catgtacaag acggccggca tcaccttcgc gggcctcgcg
      241 ctcctgctca tcacgggcgc ggtgtgggcc aacgagtcgt gggggcgcta ctgggggtgg
      301 gacccgaagg aggtcggcgc gctcgtcgcg tggctgacct acggcggctt cctccacgtc
      361 cgcatctcgc gcggctggac ggggcgcccg gcggcctact tcgcgctcgt cgccttcctg
      421 ctcatcctct tcacctacct tggcgtcagc tacctgctgc cgggcatgca ctcttatgcg
      481 tgataagtga tgagtgataa gtgatgagaa agaacaggag ccgctttctt ccttcttatc
      541 actcatcact tatcacttgt cacggggttt caaaatgaca ctcgtcctga caggtgtgag
      601 ccacaagacg gcgccggtcg aggtgcgcga gcgcctcgcc ttcgcgaagg aggcactgcc
      661 cggatacctg agcgcgctcg ttgatggccg cgcggtgctg gaagggatga tcctttcgac
      721 gtgcaaccgg gtcgagctgc tggttgacgt tgacccggcg cacttcgagg aggggaggtc
      781 gcgcgtcgcg gacttcctcg cgtcgagggg cgttggctta ggcgaagacc tcggccgtca
      841 cgtctacgag tacgctggtg aagaggccgc gcgccacctc ctccgcgtgg cctcttcgct
      901 cgactcgatg gtgttggggg agccgcagat tctcgggcag gtgcggcagg cttacgccga
      961 cgcctgcgcg gccgggaccg cgggccgcac actccaccgc ctgctccacc acgccttcca
     1021 cgccgccaaa cgcgtgcgca ccgagacgaa catcgcttcg gcggccgtct cggtctccta
     1081 cgcggcggtc gagctggggc gcaaaatcct cggcacgctc gaagggcgca ccgtgctcct
     1141 cgtcggcgcg ggcgagatgg ccgaactcgc cgcacgccat ctggcgaagg ccggggccgc
     1201 gcgcattctc gtcgccaacc gcacacacga gacagcgcac cggctcgcct cggagttcgg
     1261 cggcgaggcc gtcccctacg agagcttccc gtcgtttctg gccgaggccg acatctgtat
     1321 atgctcaacg ggcgcacgca aatatcttgt cacggcgcag atggcgcggg tggctttgga
     1381 gttgcgcggc aaccgcccgt cgttcttcat cgacatcagc gtcccgcgca acgtcgatcc
     1441 gcgtgtgggc gaggtcgcga accttttcgt cttcgacgtg gacgacctcg aatcggtcgt
     1501 cgcctccaac ctgtgcgagc gcgcgcgcga ggccaagcgg gccgagcaga tcgtcgaggc
     1561 ggagacggct cagtttcaca aagccatgcg cgccctcgaa atcggccccg tcatcgcctc
     1621 gctcaaagga aagatgcggc gcatcgccga ggaggaattc cggcgccagc gagcgcgcct
     1681 cggcgacctc accccggagc aggagcgcgc cgtcgagcag atgctcgcgg cgaccgtcaa
     1741 caggatttcg cacccgctca tcgagcgcct gcgccgctcc ttcgacaccg gcgacgccgg
     1801 cagcgtcggc gcgtggcgcg tgagcttcgg cctcgaggaa gagttggaaa ttgtgtcgtc
     1861 ggtgaagacc catctcgccg tcgctttcaa acttcaagcg gtaaccacat gagagacgaa
     1921 cttatcattg gttcgcgggg gtcgaagctc gcgctctggc aggccgagtg ggtgcgcgct
     1981 cggctcgcgg ccctcagccc gcgcctcgat gtgcgcgtcg aagtcgtcag tacttcgggt
     2041 gacgtcctga aggacgcgcc gctatcggtc atcggcgggc agggggcctt caccaaagaa
     2101 ctcgaacacg cgctcctcga acgccgcatc gacctcgccg tccactcgct gaaagacctg
     2161 ccgacggtca tccccgaccg tctcaccctc gccgccacgc ccgcgcgcga agacgcgcgc
     2221 gacgcgctcg tcctgcccgc cggcacttcc ggcggcggag tggtttccgt tcttgatctt
     2281 ccagcgggcg cgcgcgtcgg cacgtcgagc ctgcgccggc gggcgcagct tcggcacctc
     2341 aggcccgacc tcgtgttgaa tgacctgcgc ggcaacgtgg acacgcgcct ccggaagctc
     2401 gacgccggcg agttcgacgc catcgtcctc gccgcggcgg gcttgcgtcg tctcggactc
     2461 gccgggcgca tcagcgcgca cgtctcgccc gacgagctgc tgccggcggt cggccagggc
     2521 gccctcggcc tggaggcgcg cgccgacgac gaggagacac tcagccttgt cgcccgactg
     2581 gatcacgccc cgacacgcgc agcgtgtgag gctgagcgtg cactgctgcg ccagttcggc
     2641 gcgggctgtc aggtgcctat agcggcgcac gcgaccgttg aagggttgtt tctcaagttg
     2701 gaaggactcg tcgccgcgcc ctctggcgaa gacgtcctga aggcttcggt ggtcggtttc
     2761 atgggcgacg cggagggcat tggtgaagag cttgcgcacc gtctcatggc acgcggcgcc
     2821 gccttccttc tcgcgggcgc ggcgacggcg ctggcgtcct gatgggaagc ggcgcggccg
     2881 caagtactta ggcaaaaaca aacggggcgg cgcaaagtga gcgagagaag gccgccggac
     2941 ttcgaggtga gcaaatgaag aaagagaaca tcctgtactg catcatcggc gcgctgctcg
     3001 gcttcatcgt cggcttcggc ttcgccaaca ccaccaacca gcgcggctac gtggcacagg
     3061 cggccgccgc cgcgggcggt cagccaggac agatggcggg cctcccgccc aaccacccgc
     3121 ctatcgaggg cgccgccgcc gc
//
LOCUS       DASVES010000245        11540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_631061, whole genome shotgun sequence.
ACCESSION   DASVES010000245 DASVES010000000
VERSION     DASVES010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631061"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..861)
                     /locus_tag="VF240_10050"
     CDS             complement(334..861)
                     /locus_tag="VF240_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7175588.1"
                     /translation="MKLDFYVERLSANRDVFASMLRGVSDEQARWKPAAEKWSLVEVV
                     NHLHDEEREDFRTRLDIALHRPADSWPPIDPVGWVAARGYHERDLQESLQNFLRERDA
                     SLEWLRSLDAPDWHQRTAHPRGHTLSAGDLLASWLAHDLLHVRQMTRLQWEYTAAVSS
                     PHSTDYAGAWPTGGK"
     gene            complement(893..1306)
                     /locus_tag="VF240_10055"
     CDS             complement(893..1306)
                     /locus_tag="VF240_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175589.1"
                     /translation="MRRANLFHVVFGLLVVVAFVLTGQYMDRLHEHLRGMADGQRMLY
                     RSRHIYILLAGLLNLGLGTYLQLRPARWQRVLQVLGSVLIVASTCMLVAAFFREPPLA
                     DLQHTPLSYYGLHAILAGTLLHLLGGVSLKRRAGD"
     gene            complement(1378..2136)
                     /locus_tag="VF240_10060"
     CDS             complement(1378..2136)
                     /locus_tag="VF240_10060"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX7175590.1"
                     /translation="MRYFASLLLTLGLLLFPAAVSSNSSDDITKELITSQGRKRAYYL
                     YVPKTVKADKPAPLLVTLHGSGRNGLSLVEKWKDLAKKEGFIVVGPDSIDNRQWQIPL
                     DGPDFLRELVESLKTTHPINARRVYLFGHSAGAGQALSLSLLESEYFAATAVHAGAMG
                     PEANQYMEQAKRKTPIAIFVGTNDAFFPLSTVRATRDALNSRGFSAQLTEIKDHTHWY
                     YDRAPEINRDAWEFLKKHELSGEPRYEQYQWANE"
     gene            complement(2321..3601)
                     /locus_tag="VF240_10065"
     CDS             complement(2321..3601)
                     /locus_tag="VF240_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7175591.1"
                     /translation="MNNGRRYFLKRGGVALASIGLTGVAPSFLQRAVFAAGDTKHVGG
                     RRKTLIAIFQRGAVDGLNMVVPHGESAYYDLRPTIAIARPRGGVGGESVIDLDGFFGL
                     HPSLLPLKSLWDEKRLAVVHASGSPDNTRSHFDAQDYMESGTPGRKSTPDGWLNRYLQ
                     ARPDARSTTFRAVAMTQNLPRALQGRAPALAIPNLAEFSIRAGRQAGGVQGGFEDIYE
                     QSASDALRGTGKETFEAVRFLKEVNPAQYRAENRAVYPRGRFGEAMLQIAQLVKAGVG
                     LEVAFTEMGGWDTHSNEGGARGQLSNLLQQFGQGIAALARDLGPARMDDVVILTMSEF
                     GRTARQNGTGGTDHGHANAMFVVGGAARGGRVYGDWPGLRPEQLHEGRDLALTTDFRD
                     VFAEVATKHLGQVNTQAIFPGHASSPAKFRGVLG"
     gene            complement(3702..5816)
                     /locus_tag="VF240_10070"
     CDS             complement(3702..5816)
                     /locus_tag="VF240_10070"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX7175592.1"
                     /translation="MKAAKNLRRTKSIGRGQRTASVIVMLALLSNVLPAFAVGASEGQ
                     AASKEKAGKLSDEQRAVHVLNRLSFGPRAGDVERVLKLGVGRYIEEQLNPERIDDRAA
                     EGRLANLSTLGMSNSELLAKFPNPGMLVRQLQRRGELPADLADVAAARRQGNQQQPPP
                     NAPTGEAQSRMSAPGSMPVPDADANRNEYRRAIMRYMRENGMEPPARIMAELQASRIL
                     RAAYGERQLQEVMVDFWSNHFNVFAGKGADRWFLTSYDRDVIRPNALGNFRDLLEATA
                     KSPAMLFYLDNFQSVSPDAQTPRRRAGLGELFGAGGTRRNTDPERAARRQRRLTRGDG
                     DDTAMTRPQPPQQRRQRGINENYARELMELHTLGVEGGYTQKDVQEVARAFTGWTIIA
                     PRGARGMEGVAERAGTFVFLPRLHDSNEKTVLGQKIAAGGGMEDAQKVLDILVRHPST
                     ARFVAAKLARRFVSDNPPPALVERVAAAFTKSGGDIKDTLRALFAAPEFDAAEARRAK
                     IKTPFELAVSALRALGAETNGGPALQQWLARMGEGLYQYQAPTGYPDTAEHWVNTGGL
                     LERLNFALALASNRIPGTRVDLARVAGESAAPGRSVDHARVVEQFVALVLHGEMSEKS
                     RAVLLKQLSEPAGAPDASAPTQQESAPTADAMTTRGPRAGGGRFGRGQGRAQQADLAA
                     TANPEVARIAALVLGSPEFQRQ"
     gene            complement(6060..6482)
                     /locus_tag="VF240_10075"
     CDS             complement(6060..6482)
                     /locus_tag="VF240_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175593.1"
                     /translation="MTEENTRSTKTVLVVDDSHDNLVLVSLALQDKGYRVVTASNGEE
                     AVASAKIAGPDLILMDIAMPQLDGLEATRRIREQEGNERIPVIALTAFDTDGFRKAAH
                     DAGFSGYLTKPIDFDRLFNLIRMLLPERRETSSLAFQE"
     gene            complement(6604..8055)
                     /locus_tag="VF240_10080"
     CDS             complement(6604..8055)
                     /locus_tag="VF240_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX7175594.1"
                     /translation="MPLPRKLTRRVLLSSALIAFLCGQLFPAAPAAAQGGGGRRRAAA
                     ARGDARGPAARRDPAVAAMLREIDAGRIEANIRKLVSFGTRNTLSRQDDPARGIGAAR
                     DWIFAEMQRYGRDSGARLKVELQSFEQEPGRFPRIAARTRLTNVVATLPGTRGESAAR
                     VYVVSGHYDSMCSSPTDGECDAPGANDDASGTAAVMELARVMSKRKFDATIVFMAVAG
                     EEQGLVGANYYAEQAKKNGTDIEAMFTNDIVGNTVGGNGVRDPSTVRVFSEGVPSNES
                     EQEATTRRSVGGENDSPARQLARFVRETAARYLPAFRVQMIYRRDRYGRGGDHIPFLE
                     RGYAAVRFTEPHEDYTHQHQNVRTEGGVRYGDLPEFVDFAYVAQVARVNCAALAELAL
                     APARPKSAAISTRRLSSDTDLQWEANKEADLAGYEVVWRDTTSPVWTHTQWVGRATTH
                     TAKGMSKDNYFFGVRAVDAEGHRSPVSYPRPAR"
     gene            8181..10964
                     /locus_tag="VF240_10085"
     CDS             8181..10964
                     /locus_tag="VF240_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX7175595.1"
                     /translation="MPITPGTTLGPYEILSPLGAGGMGEVYRARDTRLGRDVALKVLP
                     AAFASHEERLRRFEQEAGAASALNHPNIITVHEIGHADATYFIATEFIDGETLRQRAS
                     RAGLTLRETLDVSIQAASALVAAHKAGIVHRDIKPENVMVREDGIVKVLDFGLAKLGE
                     SADGSGRAVDTEAETRGLVNTAPGVVLGTAYYMSPEQARGQEVDARSDIFSFGAVVYE
                     MLTGRRPFEGKTRSDILVSVLRDEPAPLEAYFPGLPPELSRIVSKALRKDREERYQTS
                     KELLVDLRSLKQELEFEAKAGRPNLLDEGRPAGAGRRGGPRGGATSRAAHSTAVPQHE
                     TFPSTISELLISEVKQHRKGVTLTAVLVALALAAAGFGLYRLIRSARRPDSFQTMRLA
                     KLTHAGNVVAGLVAVSPDGRQVAYVTQDEGQRSLWVRQVATPGNVQIVAPSPVEYGGL
                     TFSRDADYVYYIAAEKQGFAALYRVPALGGGPPRKLIEGIDGPITFSPDGSRLAFVRR
                     RVDLLVANADGTGAETLASRPRGEIWWQPAWSPDGNSIACGVYSPADNLWRLVEVSVK
                     DGAQKPLSPRPWQTLNGMAWLPGGDGLLVNGRDPETRQPQIWLLGYPGGEPRRVTNDL
                     SSYFGLSLTADGRTLASVQGERLSNIWVAPGGDADLARRVTFEVGRDEGLTGLALTPE
                     GRIVYATGAGGARDIWIVDEDGRNNRQLTADSRANYSPAVTPDGRHVVFVSDRAGRDQ
                     IWRMDADGGNPVQLTDGPGAAGRPNCSPDGRWVVYQVLLENKNTVWKVSIEGGPPVQL
                     TDVASFKPTVSPDGRFIVCSYGGAQAGSPIRLAVIPAEGGPPVRIIDAPAVIRSIVFR
                     WTPDGDRIVYIDSRNRVYNLWAQPLDGGPPVQLTDFKSDQIFQFDWSRDGKRLALARG
                     HDGSDVVLISDFR"
     gene            11222..11461
                     /locus_tag="VF240_10090"
     CDS             11222..11461
                     /locus_tag="VF240_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175596.1"
                     /translation="MAEAEISPFHDQTLADATQQIKQVLARIPPGPEGSHLSFLRTPR
                     GILLAWAHHRAEAPPDAVPATADEKTLSKALGLKH"
BASE COUNT         1845 a         3835 c         3946 g         1914 t
ORIGIN      
        1 aagatttttt gactatctcg acgaaaaaac ttgcggccca tgcgggagta cacgccgccg
       61 cgctcgctct cacggcgcga gacgacccgg tgaaccgttg ggggaaggtt ttggggaaat
      121 tcgggaggat attagcacgc gccgccgggg cgttccaatc cgtgccgcgc cgggtcgtgt
      181 cctgagatca actctctgcg cgtcctttgc gctctccgcg gctcggcggt gagtacccgc
      241 tcaagcacac tcaccgccga gtcgcggaga gcgcaaagga cgcgcagaga acatactcga
      301 atgaggacac tactccgcgc cggccgctct ccttcacttg ccgcccgtcg gccacgctcc
      361 cgcgtaatcc gtcgagtgcg gcgaggagac ggcggccgtg tactcccatt ggagccgcgt
      421 catctgccgg acgtgcagca ggtcgtgagc gagccatgag gcgaggaggt cgccggcgga
      481 gagcgtgtgg ccgcgcgggt gcgccgtgcg ctgatgccag tcgggcgcgt cgagcgagcg
      541 cagccactca agcgatgcgt cgcgctcgcg caggaagttt tgcaacgact cctgcaagtc
      601 tctctcgtgg tagccgcgcg cggcgaccca gccgacgggg tcgatgggcg gccacgagtc
      661 ggcggggcgg tggagcgcga tgtcgagacg cgtgcgaaag tcttcccgct cctcgtcgtg
      721 gagatgattg acgacctcga cgagagacca cttctccgcc gcgggcttcc accttgcctg
      781 ttcgtcgctc acgccgcgca gcatcgacgc gaacacgtcg cggttcgccg acaggcgttc
      841 gacgtagaaa tcaagcttca ttccggtgtc ctcttcgcct cgtgcgggcc gtttagtccc
      901 ccgctcttct cttcaaactc acgccgccga gcaggtgaag cagagtgccg gcgaggatcg
      961 cgtgcagccc gtagtagctc agcggggtgt gttgcagatc ggccagcggc ggctcgcgga
     1021 agaaggcggc gacgagcatg caggtggacg cgacgatgag gacggagccg agcacctgta
     1081 agacgcgttg ccagcgcgcc ggccggagtt gcagataagt ccccagcccg aggttgagca
     1141 gcccggcgag caggatgtag atgtgccgcg agcgatagag catccgctgg ccgtcagcca
     1201 tcccgcgcag gtgctcgtgc agacgatcca tgtactgccc cgtcaacaca aacgcgacga
     1261 cgacgagcag cccgaagacg acgtgaaaca gattcgctct ccgcactcgc tctcacggcc
     1321 ccttccgaat ctcgggcgac ctcgtcggcg actgccgcag cttgtcggcc cctgtggtca
     1381 ttcgttcgcc cattggtact gttcgtatcg aggctccccc gacagctcat gcttcttcaa
     1441 aaattcccaa gcgtcgcggt tgatctcagg tgcgcggtcg taataccagt gggtgtgatc
     1501 cttgatctcc gtgagttgcg cggagaagcc gcggctgttg agtgcgtcgc gggtggctcg
     1561 aacggtggag agcgggaaaa acgcgtcgtt cgttccgacg aagatggcga tgggcgtctt
     1621 gcgcttcgcc tgctccatat actgattggc ttccggcccc atggcgccgg cgtgcacggc
     1681 cgtcgccgcg aaatactccg actccagcag tgaaagagag agagcctgcc cggccccggc
     1741 cgaatgcccg aacagataga cgcggcgcgc atttatcggg tgcgtcgtct tcaacgactc
     1801 gaccagctct cggagaaagt ccggcccgtc caatggtatc tgccattgcc ggttgtcgat
     1861 ggaatcaggc ccgacgacga tgaacccttc cttcttcgcc aggtctttcc acttctcgac
     1921 gagcgacagg ccgttgcgcc ccgacccgtg cagcgtcacg agcagcggcg ccggcttgtc
     1981 cgccttgacg gtcttcggga cgtataggta ataagcgcgc ttcctgccct gcgacgtgat
     2041 gagttccttg gtgatgtcgt cggacgagtt tgacgagacc gcggccggga atagaagcaa
     2101 tccaagcgtc aagaggagcg aagcgaagta cctcataaat tctccttgcc actcacttac
     2161 gtcgtgaaag taagccaccc ggcggagacg accatcggtc gatgagtaac gccgggcgga
     2221 gcgcggatta taccgcatcg gcccatcgcg cccccgtaaa aaacaggggc gcggcgaatc
     2281 atcgccgcgc ccctgtttgc gcccgcccgg agagacgccg ctagccgagc acgccgcgga
     2341 acttcgcggg gcttgaggcg tgaccgggga agatggcctg cgtgttcacc tgcccgagat
     2401 gtttggtggc gacctcggcg aacacgtcgc ggaagtcggt ggtgagcgcg aggtcgcggc
     2461 cctcgtggag ttgttcggga cgcaggcccg gccagtcgcc gtaaacacga ccgccgcgcg
     2521 ccgcgccgcc gacgacgaac atcgcgttgg cgtggccgtg gtcggtgccg cccgtcccgt
     2581 tctgccgggc ggtgcgtccg aactccgaca tcgtgagaat caccacgtcg tccatgcgcg
     2641 ccgggccgag gtcgcgcgcg agggcggcga tgccctgtcc gaactgttgg aggagattcg
     2701 agagctggcc gcgcgcgccg ccttcgttgc tgtgcgtgtc ccatccgccc atctctgtaa
     2761 aggctacttc caaaccgacg cccgccttga cgagctgcgc gatctggagc atcgcctcgc
     2821 cgaatcgccc gcgcgggtag acggccctgt tctccgcgcg gtactgcgcc gggttaactt
     2881 ccttcagaaa cctgaccgcc tcgaacgtct ccttgcccgt accgcgcagc gcgtccgacg
     2941 cgctctgctc gtagatgtcc tcgaagccgc cctgcacgcc gcccgcctga cggcccgcgc
     3001 ggatggagaa ctcggcgagg ttcgggatgg cgagcgcggg ggcgcgtccc tgaagcgcgc
     3061 gcggcagatt ctgcgtcatc gcgacggcgc ggaaggtcgt ggagcgcgcg tcggggcgcg
     3121 cctgaaggta tcggttgagc cagccgtcgg gcgtggactt gcggcccggc gtgccggact
     3181 ccatgtagtc ctgcgcgtcg aagtgtgagc gcgtgttgtc gggcgacccc gaggcatgga
     3241 cgacggccag acgcttctcg tcccacagcg acttgagcgg cagaagcgac gggtggaggc
     3301 cgaagaagcc gtcgaggtcg atgaccgact cgccgcccac gccgccgcgt ggccgcgcga
     3361 tggcgatggt cgggcgcaag tcgtagtaag cgctctcgcc gtgcggcacg accatgttga
     3421 ggccatccac cgcgccccgc tggaagatgg cgatcaaggt cttgcgtctg ccgcccacgt
     3481 gcttcgtgtc accggccgcg aacaccgcac gttgtaggaa agatggcgcg acgcccgtca
     3541 acccgatgct cgccagcgcg acgccgcccc gcttcagaaa atatctacga ccgttgttca
     3601 tctccacctc tcggaagagt gataagtgat gagtgataag tgataagaaa atcggcttgc
     3661 tctttctcat cacttatcac tcatcactta tcaccgtctc tttactgcct ctgaaattcc
     3721 ggcgacccga ggacgagcgc ggcgatgcgc gcgacttcgg ggttggcggt cgcggcgagg
     3781 tcggcctgtt gcgcgcggcc ttgaccgcgt ccgaagcgac cgccgccggc gcgcggcccg
     3841 cgcgttgtca tcgcgtcggc agtgggcgca ctctcctgtt gcgtgggcgc ggaggcgtcg
     3901 ggggcgcccg ccggttcgct caactgcttg aggaggacgg cgcggctctt ctcggacatc
     3961 tcgccgtgga gaacgagcgc gacgaactgc tcgacgacac gcgcgtgatc gacggagcgg
     4021 cccggcgcgg ccgactcgcc cgcgacgcgc gcgagatcaa cgcgcgtgcc cggaatgcgg
     4081 ttgctcgcga gcgcgagcgc gaagttgagt cgttcgagga ggccgcccgt gttgacccag
     4141 tgctcggccg tgtcggggta acccgtgggc gcctgatact ggtagagacc ctcgcccatg
     4201 cgcgcgagcc actgttgaag cgcggggccg ccgttcgttt ccgcgccgag cgcgcgcagg
     4261 gccgagacgg ccagctcgaa cggcgtcttg atcttggccc ggcgcgcttc cgccgcgtca
     4321 aactccgggg cagcgaagag tgcgcgcaga gtatccttga tgtcgccccc agacttcgtg
     4381 aaggccgcgg cgacgcgctc gacgagcgcg ggcggcgggt tgtcggagac gaaacggcgc
     4441 gcgagcttcg cggcgacgaa gcgcgccgtc gagggatggc ggacgaggat gtcgagcacc
     4501 ttctgcgcgt cctccatccc accgcccgcg gcgatcttct ggccgagcac ggtcttctcg
     4561 ttcgagtcgt ggagacgcgg gaggaagacg aaggtgccgg cgcgctccgc aacgccctcc
     4621 atcccgcgcg caccgcgcgg ggcgatgatc gtccagccgg tgaaggcgcg cgccacctcc
     4681 tgcacgtcct tttgcgtgta gccgccctcg acgccgagcg tgtgcagctc catcagctcg
     4741 cgcgcgtagt tttcgttgat gccgcgttga cgacgctgtt gcggcggctg cgggcgcgtc
     4801 atcgccgtgt cgtcgccgtc gccgcgagtc aggcggcgct ggcggcgtgc cgcgcgttcg
     4861 gggtcggtgt ttcggcgcgt gccacccgcg ccgaagagtt cgccgaggcc ggcgcggcgt
     4921 cggggcgtct gcgcgtcggg gctgacgctc tggaagttgt cgaggtagaa aagcatcgcc
     4981 ggacttttgg cggtggcttc gaggaggtcg cggaagttgc cgagcgcgtt ggggcgaatc
     5041 acgtcgcggt cgtaggaagt caggaaccag cggtcggcgc ccttgccggc gaagacgttg
     5101 aagtggttcg accagaaatc aaccatcact tcctgcaact gccgctcgcc gtaagcggcg
     5161 cgcaggattc tcgacgcctg tagctcggcc atgatgcgcg cgggcggctc catgccgttc
     5221 tctcgcatgt agcgcatgat ggcgcgtcga tactcgttgc ggttcgcgtc cgcgtcgggc
     5281 acaggcatcg agccgggcgc gctcatccgc gactgcgctt cgccggtcgg cgcgttcggc
     5341 ggcggttgct gttgattgcc ctgccgacgc gccgcggcca cgtcggccaa gtccgccggc
     5401 agctcgccgc ggcgctgcaa ctgccggacg agcataccgg ggtttgggaa cttcgccagc
     5461 agctcgctgt tcgacatccc gagcgtggaa aggttcgcga gccggccttc ggcggcgcgg
     5521 tcgtcgatgc gctcggggtt gagctgttcc tcgatgtagc gaccgacgcc gagcttgagc
     5581 acgcgctcca cgtcgcccgc gcgggggccg aaagacagac ggttgagcac gtggacggcg
     5641 cgctgctcgt cgctcagctt gccggccttc tccttagacg cggcctgccc ctccgacgcg
     5701 cccacggcga acgccggaag gacgtttgag agcagcgcca acatgacgat gacagaggcg
     5761 gtgcgctgac cgcgtccgat tgatttagtg cgacgaaggt tctttgcagc tttcataact
     5821 actttgctcg aacgtcctga aaattataaa aaatcgggtg gttgaagtct cctgcctgcc
     5881 gtccccaagt tagacgcggg acggctcctg atggttgaaa aagattggtt caatgaaggc
     5941 gggcacgcac agtaccgcga gcggtagcga gcgggtctgg atgcgcgatg ctggcctctt
     6001 gtcgcaggcg tacccgctcg ctaccgctcg cggtactgtg attgagtcaa tgagccatct
     6061 cattcctgaa aggccagaga gctggtctcg cggcgctcgg gcaggagcat gcggatgagg
     6121 ttgaagaggc ggtcgaagtc gatgggcttg gtgagatagc cgctgaagcc ggcgtcgtgg
     6181 gcggccttgc ggaagccgtc ggtgtcgaag gcggtgagcg cgatgacggg gatgcgctcg
     6241 ttgccctcct gctcgcggat gcggcgcgtg gcttcgaggc cgtcgagctg cggcatggcg
     6301 atgtccatga ggatgaggtc ggggccggca atcttggccg aggccacggc ctcctcgccg
     6361 ttgctggccg tcaccacgcg gtagcccttg tcctgcaacg ccagactcac cagcacgagg
     6421 ttgtcatgcg agtcgtcaac gacgaggacg gtcttggtgc tgcgcgtgtt ctcttcggtc
     6481 attttcggtc tatctctaca cccgttttag atgctcgcgc aagctcaacg ggtttgcagc
     6541 aaagttcggg cacgcagacg attcacaaac tcacggcgac ggggcgcagc gcggccgtcc
     6601 cctttagcgc gcggggcgcg gatagctgac ggggctgcga tggccctccg cgtctacggc
     6661 gcgcacgccg aagaagtagt tgtccttcga catccccttc gccgtgtgcg tggtcgcgcg
     6721 cccgacccac tgcgtgtgcg tccagacggg cgaggtcgtg tcgcgccaga cgacttcgta
     6781 gccggcgagg tctgcctctt tgttcgcctc ccactgcaag tcggtgtcgc tggagagccg
     6841 gcgcgtcgag atggcggccg acttcgggcg cgcgggggcg agggcgagtt cggccagtgc
     6901 ggcgcagttg acacgcgcga cctgcgcgac gtaagcgaaa tcgacgaact cgggcaggtc
     6961 gccgtagcgc acgcctccct cggtgcggac gttctggtgc tggtgcgtgt agtcctcgtg
     7021 cggctcggtg aagcgcacgg ccgcgtagcc gcgctcgagg aatgggatgt ggtcgccgcc
     7081 gcgcccgtag cggtcgcggc gatagatcat ctgcacgcgg aaagccggaa gataacgcgc
     7141 ggcagtctct cggacgaagc gcgcgagctg gcgggcgggc gaatcgttct cgccgccgac
     7201 gctgcggcgc gtcgtggcct cctgctccga ctcgttcgac ggcacgccct cggagaaaac
     7261 gcgcaccgtg gagggatcgc gcacgccgtt gccgcccacg gtgttgccga cgatgtcgtt
     7321 cgtgaacatc gcctcgatgt cggtgccgtt cttcttcgcc tgctcggcat aatagttcgc
     7381 gccgactaag ccctgctcct cgccggcgac ggccatgaag actatcgtcg cgtcgaattt
     7441 tcgtttcgac atcacgcgcg ccagctccat gacggcggcc gtgcccgagg cgtcgtcgtt
     7501 ggcgcccggc gcgtcgcatt cgccgtcggt cggactggag cacatcgagt cgtagtgccc
     7561 gctgacgacg tagacgcggg ccgcggactc accgcgcgtt ccgggcagcg tggcgacgac
     7621 gttggtgaga cgcgtccgcg cggcgatgcg cgggaagcgt cccggctcct gctcgaagga
     7681 ttgcagctcg accttgagac gagcgccgga gtcgcggccg tagcgttgca tctcggcgaa
     7741 aatccagtcg cgcgccgcgc cgatgccgcg cgccgggtcg tcctggcgcg agagcgtgtt
     7801 gcgtgtgccg aaagatacga gcttgcggat gttggcctcg atgcgcccgg cgtcgatctc
     7861 acggagcatg gcggccaccg cggggtcgcg gcgcgcggcg gggccgcgtg catcgccgcg
     7921 tgccgccgca gcccgacgcc gccccccgcc gccttgcgcg gcggccggcg ccgccggaaa
     7981 gagttggccg cagaggaatg cgatcagtgc tgacgacagg aggacgcggc gcgtgagctt
     8041 gcgcggcaga ggcatacggg ctgtttcctt tctcacacgg gcgtgacgaa cgggggcgaa
     8101 caaactcttg aatgaccggg cacgggagcg agtataatat gccggctcgc tcgcaaactc
     8161 cacaagggga cgcccccgaa atgcccatca cccccggcac cacactaggc ccttacgaaa
     8221 tcctctcgcc gctcggcgcg ggtgggatgg gcgaggtcta ccgggcgcgc gacacgcggc
     8281 tcgggcgcga cgtggccctc aaggttctgc ccgccgcctt cgcatcccac gaggagcgtc
     8341 tgcgccgctt cgagcaggag gcgggcgccg cctcggccct caaccatccg aacatcatca
     8401 ccgtccacga gatcggacac gccgacgcga cctacttcat cgccaccgag ttcatcgacg
     8461 gcgagacgct gcggcaacgc gcgtcgcggg cggggcttac gctgcgcgag acgctcgacg
     8521 tctcgatcca ggcggcctcc gcgctcgtcg ccgcgcacaa ggccggcatc gtccaccgcg
     8581 acatcaagcc cgagaacgtg atggtgcgcg aggacggcat cgtcaaggtg ctcgacttcg
     8641 ggctggcgaa gctgggcgag agcgccgacg ggtcggggcg tgcggtggac acggaggccg
     8701 agacacgcgg gctcgtcaac accgcgcccg gcgtcgtgct ggggacggcc tattacatgt
     8761 cgcccgaaca ggcgcgcggt caggaggttg acgcgcgcag cgacatcttc tcgttcggcg
     8821 cggtcgtcta cgagatgctc acgggacgca ggccgttcga ggggaagacc cgaagcgaca
     8881 tcctcgtctc cgtcctgcgc gacgagccag cgcccctcga agcatacttc cccggcctcc
     8941 ccccggagtt gagccgcatc gtctcgaagg cgctcaggaa ggatcgcgag gagcgatacc
     9001 agacgagcaa ggagctgctg gtcgatctgc ggagcctcaa gcaggagctt gagttcgagg
     9061 ccaaggcagg ccgcccgaac ctcctcgacg agggccgccc cgcgggcgcg ggccggaggg
     9121 gcggccctcg cggcggcgcg acgagccgcg ccgctcactc gacggccgtc ccccaacacg
     9181 agacgttccc ctcgacgatc agcgagctgc tcatcagcga agtcaaacaa catcgcaagg
     9241 gcgtcacgct gacggcagtg ctcgtcgcgc tcgcgctcgc cgccgctggc ttcggcctct
     9301 accggctcat ccggtcggcg cggcggcccg actccttcca gacgatgcgg ctcgccaagc
     9361 tgacgcacgc gggcaatgtc gtcgccgggc tggtcgccgt ctcgcccgac ggccggcaag
     9421 tcgcctacgt gacgcaggac gaggggcagc ggagcctctg ggtcaggcag gtggcgaccc
     9481 ccggcaacgt gcagatcgtc gcgccgtccc cggtcgaata cggcgggctg acgttctccc
     9541 gcgacgccga ctacgtctac tacatcgccg ccgaaaagca gggcttcgcc gcgctctacc
     9601 gcgtgcccgc gctcggcggc ggcccgccgc gcaagttgat tgaggggatc gacggcccca
     9661 tcaccttctc gcccgacgga agtcgcctgg ccttcgtgcg aaggagagtc gatctgttgg
     9721 tcgctaatgc tgatggcaca ggggccgaga cgctcgcctc gcgcccgcgc ggtgaaatct
     9781 ggtggcagcc cgcctggtcg cctgacggaa acagcatcgc ctgcggcgtc tactcgccgg
     9841 ccgacaacct ctggcggctg gtcgaggtct cggtgaaaga cggggcgcag aagccgctct
     9901 cgccgaggcc gtggcagacg ttgaacggga tggcgtggct gcccggaggc gacgggctgt
     9961 tggtcaacgg gcgcgatccc gagacccggc agccgcaaat ctggctcctc ggctatccgg
    10021 gcggcgagcc gcgtcgagtg accaacgacc tgtcgagcta cttcgggttg agcctgacgg
    10081 ccgacgggcg gacgctggcg agcgtgcagg gcgagcggct gtcgaacatc tgggtcgcgc
    10141 ccggcggcga cgccgatctc gcacgccgcg tcaccttcga ggtgggcagg gacgagggac
    10201 tgaccggcct cgcgttgacg ccggaggggc gcatcgtgta cgccacgggg gccggcggcg
    10261 cgcgcgacat ctggatcgtc gatgaggacg gacgcaacaa ccgacaactg accgccgact
    10321 ctcgcgccaa ctactcgccg gcggtcacgc cggacgggcg ccacgtcgtc ttcgtttccg
    10381 accgcgcggg ccgcgatcaa atctggcgca tggacgccga cggcggcaac cccgtacagt
    10441 tgaccgacgg ccccggcgcg gccggcaggc ccaactgctc gcccgacggc cggtgggtcg
    10501 tctatcaggt cttgcttgag aacaaaaaca ccgtctggaa ggtgtcgatt gagggcgggc
    10561 cgcccgtgca actcaccgac gtggcatcgt tcaagcccac ggtgtcgccc gacgggaggt
    10621 tcatcgtctg ctcttacggc ggtgctcagg ccggctcgcc catcaggctg gccgtcatcc
    10681 ccgccgaggg agggccgccc gtgcggatca tcgacgcgcc ggccgtcatc aggtcaatcg
    10741 tcttccgctg gacgccggac ggcgaccgca tcgtctacat cgacagccgc aaccgggttt
    10801 ataatctgtg ggcgcaaccc ctcgacggcg gcccgcccgt ccagttgacc gacttcaagt
    10861 ccgaccagat tttccagttc gactggtcgc gcgacggcaa acgtctcgcc ctcgcccgcg
    10921 gccacgacgg ctccgacgtg gtgctgatta gcgacttcag gtagcgcgcg ccggggagct
    10981 gcgtctttcc tcgtcccggc cctcgccagc gttcgtcgaa catacatgcg gcttcatctc
    11041 gcacggcggg ccgcggattt aagcgccggc ccgtaaacac cacgttcttt cctgcccctg
    11101 aaaggaggcc aagacaatga gtaacggaaa aaaatgcgac tccggaggcg attgcacctt
    11161 cgccgtccac cccccggtga taatctgtct accgggcggc agtgcgtgcg ggctcgcggt
    11221 catggcagag gccgagatat ctcctttcca cgaccagacg ctggccgacg ctacccaaca
    11281 gatcaaacaa gtgctcgcgc gcatcccccc cggcccggag gggagccacc tctcgttcct
    11341 cagaacgccc aggggcatcc tgctcgcgtg ggcgcatcac cgcgccgagg ccccgcccga
    11401 cgccgtgccc gcgaccgccg acgaaaagac cctctcgaag gcgctcggac tcaagcactg
    11461 acgccccgcc cgcggccggc gatagcctct gcgcctccgg cgattgaggc ccgcggccga
    11521 attgccgaca ggcgccacac
//
LOCUS       DASVES010000246         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_586427, whole genome shotgun sequence.
ACCESSION   DASVES010000246 DASVES010000000
VERSION     DASVES010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586427"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VF240_10095"
     CDS             <1..352
                     /locus_tag="VF240_10095"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7175597.1"
                     /translation="TGNIVTPDPLPEENVFLRSDHYEFVKKGVPALMLAGGPAGDPAV
                     WIARTKKWMDTDYHSPNDVVLPDWNWSGPQTVAQVGLLIGLRVANADVMPAWLPSSPF
                     NKPRAAAATPAPGN"
     gene            complement(444..800)
                     /locus_tag="VF240_10100"
     CDS             complement(444..800)
                     /locus_tag="VF240_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX7175598.1"
                     /translation="MPYSETLKDHLARPRNAGTLADANASAEQTNPVCGDRLHLSLRV
                     SGGRIEAARFLAYGCPPTLACGSALTELIEGMTTEEATRLTRQEMIDAVGGLPARKRH
                     AAALAIETLQAALNIV"
     gene            complement(884..2098)
                     /locus_tag="VF240_10105"
     CDS             complement(884..2098)
                     /locus_tag="VF240_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX7175599.1"
                     /translation="MPLRRVYMDHSATTPVDRRVVEAMLPYLMDKFGNASSVHFFGQE
                     ARTAIDRARREVAALINARPNEVVLLSGGTEANNLAVRGICELHAEHGRHVITSAIEH
                     SAVRGVVGALEKRGWEVTRLPVYTDGVVRLEDVRAALRPDTVLITVMLANNEIGTVQP
                     VAEIGALVREQREAGRRRLWLHTDAVQALGRMTVDVDALGCDLLSMSAHKLHAPKGVG
                     ALYVRRSVRLAAQSVGGHQERERRAGTEAVELAVAFGKAAELARLELDERVEHMLTLR
                     DRFEAGIEERISDVVFNGRGAPRLPHLSNVSFRFVEGEGLLINLDMQGVAVSTGSACS
                     SGSLEPSPVILALGRTDELARGSIRFSIGKDTTDADIDYVLEVLPRAVENLRRLSPLY
                     QKALAERACAPA"
     gene            2524..3561
                     /locus_tag="VF240_10110"
     CDS             2524..3561
                     /locus_tag="VF240_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175600.1"
                     /translation="MSTLRKILNAALAAALLIVPAAAGVFAAPRVETEETSTQQQPYG
                     ALERGWRTGYSDGYQTGWRDVVDRRESDYQNKDEYRRADRAHVAAYGPVEQYRDGYRQ
                     GFELGYADGYARRGFNSELPAGGVRARGDVAESVETTEARPSSGTVREADPADDNYTR
                     DAGRTSASAAGSIPPNTILRVELLNRLSTDVSQRGDRFEARVIEPREYEGAVVSGKVA
                     EVGRAGKVNGRAQLQLDFDQIRLASGEWADFSAQVIEVINTGGSDETGEVDPEGGVRG
                     RSSTKDDVKKVGVSTGIGAIIGAIAGGGKGAVIGAVIGAGVGTGGVMTQRGKDIRFER
                     GQQLRLRTSGR"
BASE COUNT          637 a         1184 c         1261 g          574 t
ORIGIN      
        1 gaccggcaac atcgtcaccc ccgacccgct gcccgaggag aacgtcttcc tgcgctccga
       61 ccattacgag ttcgtcaaga aaggcgtgcc ggcgctgatg ctggcgggcg gccccgccgg
      121 cgaccccgcc gtctggatcg cgcgcacgaa gaagtggatg gacactgact accactcgcc
      181 caacgatgtc gtgctgcccg actggaactg gagcggcccc cagacggtcg cgcaggtcgg
      241 cctcctcatc ggactgcgcg tcgccaacgc cgacgtgatg cccgcctggc tcccctcctc
      301 gccctttaac aagccgcgcg ccgccgccgc cacgcccgcg ccgggaaact aatagcccta
      361 acggagcggc gactccgccg gacaacgaga gagggccgcg acagattgat gtctgccgcg
      421 gccctctcac tttttcgcct cgcctaaacg atattaagcg ccgcttgaag cgtctcgata
      481 gcgagcgcgg cagcgtggcg cttgcgcgcg ggcaggccgc cgaccgcgtc aatcatttcc
      541 tgacgcgtga ggcgcgtggc ctcttccgtc gtcatcccct cgatgagttc ggtgagcgcg
      601 gagccgcagg cgagcgtcgg gggacagccg taggcgagga agcgcgcggc ctcgatgcga
      661 ccgccgctca cgcggaggga gagatggagg cggtcgccgc agacggggtt cgtctgttcg
      721 gcagaggcgt tggcgtcggc gagtgtgccg gcgttgcgcg ggcgggcgag gtggtctttg
      781 agtgtttcgg agtaaggcac agagtagttc aaagttcaaa gttcaaagtt caaagtcaac
      841 agcggcacgg tgcgctttga actttgaact ttgaactttg aactcacgca ggggcgcagg
      901 cgcgctcggc caaagccttc tggtagagcg gcgagaggcg gcgcagattt tcgacggcgc
      961 gggggagcac ttcgagcacg tagtcgatgt cggcgtcggt ggtgtccttg ccgatgctga
     1021 agcggatgga gccgcgggcg agttcgtcgg tgcggccgag ggcgaggatg acgggcgacg
     1081 gctccagcga ccccgaagag caggccgacc ccgtggagac ggccacgccc tgcatgtcga
     1141 ggttgatgag cagcccctcg ccttcgacga agcggaagga gacgttcgac aggtgcggca
     1201 gccgcggcgc gccccggccg ttgaagacca cgtccgagat gcgctcctcg atgcccgcct
     1261 cgaagcggtc gcgcaaggtg agcatgtgct cgacgcgctc gtccaactcc agacgggcaa
     1321 gctcggccgc cttgccgaac gcgacggcca actccacggc ctccgtcccc gcgcgccgct
     1381 cgcgctcctg atggccgccc accgactgcg cggcgagccg cacgctccgc cggacgtaga
     1441 gtgcgccgac acctttgggc gcgtggagct tgtgcgccga catcgagagc aggtcgcacc
     1501 ccaatgcgtc cacgtcaacc gtcatccgac cgagcgcctg cacggcgtcc gtgtggagcc
     1561 agaggcgccg gcggcccgcc tcgcgctgct cgcggacgag tgcgccgatc tcggcgacgg
     1621 gctgaacggt gccgatttcg ttgttggcga gcatgacggt gatgagcacg gtgtctggcc
     1681 gcagggccgc gcgcacgtct tcgaggcgaa cgacgccgtc cgtgtagacc ggcaggcgcg
     1741 tcacctccca cccgcgcttc tccagtgcgc cgacgacgcc gcgcaccgcg gagtgttcga
     1801 tggccgaggt gatgacgtgt cggccgtgct cggcgtgtag ttcgcagatg ccgcggacgg
     1861 cgaggttatt ggcctccgtg ccgcccgaga ggaggacgac ctcgttgggg cgggcattga
     1921 tgagcgcggc cacctcgcgc cgcgcgcggt cgatggccgt gcgcgcctcc tgcccgaaga
     1981 agtggacgct cgaagcgttg ccgaacttgt ccatcaggta cggcagcatc gcctcgacga
     2041 cccggcggtc aaccggcgtc gtcgcgctgt gatccatgta gactctgcgg aggggcatgg
     2101 ggaagaagct gttagctgtt agctgttagc tcttagcttt cagcaaagag ctgttctttg
     2161 tcggcggaca acggacaact gaccagagac agcttttagc tgatagctaa cagctaatcg
     2221 ctaaaagctc tgaaaaaatt cctccaaccc gcacggaagg cgggggcatc aaaggcgcag
     2281 gcccgagcga cgcctcgtaa cgttgcgcgt gattcgagtc tacggcgcgt gccaacgagc
     2341 gtcaaggcgg ttggttttcc cggcggcgac gctccggcgg cggaagcggg ccatccattt
     2401 ttccgctcga cttggccgcg cctcggggtg aaaatagagc ggcccgcgtt tcaatatgca
     2461 cgcgcgaaga cccggcccga ccgttatcgc cgccgactgt gttcaatcag gagacgagaa
     2521 gccatgtcca ctctcagaaa gattctgaac gccgcactcg ccgccgccct gctcatcgtg
     2581 ccggcggccg cgggcgtctt cgcggccccg cgggtcgaga ccgaagagac ttccacacaa
     2641 caacagccct acggcgcgct cgaacgcggc tggcgcacgg gctattcgga cggctaccag
     2701 acgggctggc gcgacgtggt ggatcgccgc gagagcgact accagaataa ggacgagtac
     2761 cgccgcgccg accgcgcaca cgtcgccgcc tacggccccg tcgagcaata ccgcgatggc
     2821 taccggcagg gcttcgagct gggctacgcg gacggctacg cacgccgcgg cttcaactcg
     2881 gagttgccgg ccgggggcgt ccgcgcgcgc ggcgacgttg cggagagtgt cgagacgacc
     2941 gaagcgcgcc cgtcgagcgg cactgtcagg gaagctgacc cggcggacga caactacacg
     3001 cgcgacgcgg ggcgcacgtc cgcgtcggcc gctggttcga taccgccgaa cacaatcttg
     3061 cgcgtcgagc ttctgaatcg tctttcgacg gacgtgagcc agcggggcga ccgcttcgag
     3121 gcgcgcgtca tcgagccgcg cgagtatgag ggcgcggtcg tctcggggaa ggtcgcggag
     3181 gtgggacgcg ccggcaaggt caacgggcgc gcgcagctcc agctcgactt cgaccagatt
     3241 cgcctcgcga gcggcgagtg ggccgacttc agcgcgcagg tcatcgaagt catcaacacg
     3301 ggcggcagtg acgagaccgg cgaggttgac cccgagggcg gcgtccgcgg tcgcagctcg
     3361 acgaaagatg atgtgaagaa ggtgggcgtc tccacgggca tcggcgccat catcggcgcg
     3421 attgccggcg gcggcaaagg cgcggtcatc ggcgccgtca tcggcgcggg cgtcggcacg
     3481 ggcggcgtga tgacccagcg cggcaaagac atccgcttcg agcgcggcca gcaactcagg
     3541 ctccgcacct ccggcagata agaacggagt gatgagtgat gagtgataag tgataagcag
     3601 ggggatgcag ggctttttct tatcactcat cactcatcac ttatcacttt ctctct
//
LOCUS       DASVES010000247         4981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_561467, whole genome shotgun sequence.
ACCESSION   DASVES010000247 DASVES010000000
VERSION     DASVES010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561467"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VF240_10115"
     CDS             <1..252
                     /locus_tag="VF240_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7175601.1"
                     /translation="DVHDVGRYHTDDREARPVEPGIVMTVEPGIYIGTDAEGVPDEYR
                     GIGVRIEDDVLVTANGHEVLTAAAPKQIEEIESLMASAR"
     gene            338..607
                     /locus_tag="VF240_10120"
     CDS             338..607
                     /locus_tag="VF240_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175602.1"
                     /translation="MQNLHMGELGEQRWAVISERGCEAQSLPHAEAAGLMRRLKGEGI
                     SGLCVVTDEAAARLQLGAKTSPNGSKPPAPKPARQQRQQRRAAKT"
     gene            complement(910..1638)
                     /locus_tag="VF240_10125"
     CDS             complement(910..1638)
                     /locus_tag="VF240_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7175603.1"
                     /translation="MNLRIATYNVHKCRGLDRRVRPARIAAVLREVDADVVALQEVVS
                     FEDRAREQHQARFIAEELGYSYHVGECRRHQGGAYGNVTLTRLNVAEVCNYDITWRWQ
                     EPRGCLRVDVKLDDAGARLHVFNAHLGTAFIERRHQGRKLVGDAVLRNHKLTGPRVVL
                     GDFNEWTRGLASRLLAEELRSADVRQHLRRARTYPGPLPILHLDHVYYDPALHLERLW
                     LHKSLTALVASDHLPLVADFTIQS"
     gene            complement(1635..2915)
                     /locus_tag="VF240_10130"
     CDS             complement(1635..2915)
                     /locus_tag="VF240_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX7175604.1"
                     /translation="MQIDWFNVVAIIAIVVLSFLLFLALFEPGLDYKISSPYAAPLDS
                     PDFLRMLESITDSRVGDCTRVEVLTNGEVYYEAELQALRAAASSINLEAYIFQKGEVT
                     RRFLEVLTERARAGVEVRLVLDWLGSFTSWTSYFRELTEAGGRVGWYHPPRWQTIARF
                     NNRTHRELIIVDGRVGFIGGAGFADHWLIPREGHPRWRDTMFRVEGDGVASLQGTFAE
                     NWLESTGEVLSGGDFFPLCEATSHASALVVNSSPSAGRSTHARMLYQTLLASAQKSIL
                     ITTPYFLPDRSARAEMVRAIAERGVRVEIITPGKKSDHTLTRRSSRRLYGQLLEAGAD
                     IYEYQPAMLHAKTMVIDGVWSVVGSTNFDNRSFGLNDEVNLAAFDPELAARLAKDFAR
                     DRAESRAITLSEWRRRPIFERAHEQLGRILERQQ"
     gene            complement(2971..3558)
                     /locus_tag="VF240_10135"
     CDS             complement(2971..3558)
                     /locus_tag="VF240_10135"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7175605.1"
                     /translation="MATEVLEPTTEQRLTHRAASIEEFWALPESVLLTEYINGEILMA
                     PAPVPLHQFVLGNIFTALGNFARNNSLGTIFLSPVDVVLPSGDVVQPDVVFLTSRDAK
                     RALAAKRVHATPPLLVEIISPGSITHDTLRKRNLYERNGVREYWIVDPAARTISQLVL
                     RKKHYALTEHVEADAIRSAVLSGFEMNVGELLGLV"
     gene            complement(3608..>4981)
                     /locus_tag="VF240_10140"
     CDS             complement(3608..>4981)
                     /locus_tag="VF240_10140"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HEX7175606.1"
                     /translation="AFRRAIRLLVKEHAYANAEWADLVGAFERASGRKLSLWADSWVK
                     RRGMPDVRVKWSADKRGRISRLTVEQRDVLGEGGAWPMRVKLLLAPARSGRRPETLDV
                     TLSGDGVTEVREAVGRRRPSFVFANHEDYGYGRFLLDDASRAAVLKNFGAIDDEFLRT
                     LLWGALWEGVREAETGPLEYIDLAVRHAGRERDDVTLQSVLARVQTAYARYLSGEQQA
                     QVAPRLEAWLSSQMLTAETVGLRITYYRTFREVATTPAAFDKLESILTGALQVPGMTL
                     RSRDRFDIARSLLSRRGGGGDERAEALFKSVVSQDASDDGRRYAYAAEAARPVASVKN
                     KYFDDFLNDPALAESWIEAAVGPFNTPRQSELTLPYLAPALRQLPTLKRTRKIFFVNG
                     WLAAFLGGQCSPAALDTVNDFLRREPRLDRDLRLKVLEAADALERCVRVRARYASEVP
                     STRSPAP"
BASE COUNT          825 a         1540 c         1806 g          810 t
ORIGIN      
        1 gacgttcacg acgtgggccg ctatcacacg gacgacaggg aagcgcggcc cgtggagccg
       61 ggcatcgtga tgacggtcga gccgggcatc tacatcggga cggacgccga gggcgtgccc
      121 gacgagtacc gaggcatcgg cgtccgcatc gaggacgacg tgctcgtcac cgcaaatggc
      181 cacgaagtac tgacggccgc cgcgccgaag cagatcgagg agatcgagag cctgatggcg
      241 tcggcgcgct gacgggcgcc ggccgtcgcc gtcgccgctt ctggccttcg gcggcggccg
      301 tcgattaaac tcgtctcagt ccagttaaaa acggagcgtg caaaacctac atatgggtga
      361 actcggcgaa cagcgttggg ccgtgatcag cgagcgcggc tgcgaggcgc agagcctccc
      421 gcacgcggag gctgccgggt tgatgcggcg cctcaagggc gagggcatca gcggcctctg
      481 cgtcgtcaca gacgaagccg ccgcgcgtct gcaactgggc gcgaagacct ccccgaacgg
      541 cagcaagccg cccgccccaa aacccgcccg ccagcagcgg cagcagcggc gcgccgcgaa
      601 gacgtagacc aacttcgcaa ccgcgttcgc cggcagatta ccccgcatcg aaggcgacaa
      661 cgagccgcgg agcggcggca gaatgtagcc cacggcgtga gccgtgggga cacgacggtt
      721 aaaaaagaaa gccccggagg ggcgaaagaa tctcaggctt gagcgtcagt tgcgtcgagg
      781 ttctttcgcc cctccggggc ttcaaatgtt ctgtcgcgtc tattcccacg gctggcgccg
      841 tgggctacat tctgccgcgc ctaccggcgc tggtggctcg tcgtcattat cgaatgctct
      901 ggcggcgcgt cacgattgaa tcgtgaagtc cgcgacgagc ggcaggtggt cggaggcgac
      961 gagggcggtg aggctcttgt ggagccagag gcgttcgagg tgaagggccg ggtcgtagta
     1021 gacgtggtcg aggtggagga tggggagcgg ccccgggtag gtgcgcgcgc gccggaggtg
     1081 ctggcgcacg tcggcgctgc ggagttcttc ggcgagcagg cgcgaggcga gtccgcgcgt
     1141 ccactcgttg aagtcgccga ggacgacgcg cggccccgtg agcttgtggt ttctgagcac
     1201 agcgtcgccc acgagcttgc gcccctgatg gcggcgctcg atgaaggcgg tgccgaggtg
     1261 cgcgttgaag acgtggagac gcgcgccggc gtcgtctaat ttcacgtcca cgcgcaggca
     1321 gccgcgcggc tcctgccagc gccaggtgat gtcgtagttg cacacctcgg cgacgttcag
     1381 gcgcgtcagg gtgacgttgc cgtaggcccc gccctgatga cgccggcact cgccgacgtg
     1441 atagctgtag ccgagttctt cggcgatgaa gcgcgcctga tgctgctcgc gcgcgcggtc
     1501 ttcaaagctc accacttcct gaagcgcgac gacatcggcg tccacttcgc gcagcacggc
     1561 ggcgatgcgc gccgggcgca cgcggcggtc gaggccgcgg cacttatgga cgttgtaggt
     1621 ggcgatgcgg aggttcactg ctgtcgttcg aggatgcggc cgagctgttc gtgggcgcgc
     1681 tcgaagatgg ggcggcgacg ccactcggag agggtgatgg cgcggctctc ggcgcggtcg
     1741 cgcgcaaagt ctttggcgag gcgggcggcg agttcggggt cgaaggccgc gaggttcacc
     1801 tcgtcgttga ggccgaagga gcggttgtcg aagttcgtcg agccgacgac cgaccagacg
     1861 ccgtcgatga ccatcgtctt ggcgtgcagc atcgccggct gatactcgta gatgtccgcc
     1921 ccggcctcca acaactggcc gtagagccgt cggcttgagc gccgcgtcag cgtgtggtcg
     1981 ctcttctttc ccggcgtgat gatctcgacg cggacgccgc gctcggcgat agccctgacc
     2041 atctcggcgc gcgcgctgcg gtcgggcaaa aagtagggcg tggtgatgag gatgctcttc
     2101 tgcgccgagg cgaggagcgt ctggtagagc atgcgggcgt gcgtggagcg cccggccgag
     2161 ggcgagctgt tgacgacgag ggcggaggcg tggctcgtcg cctcgcagag cgggaagaag
     2221 tcgccgcccg agagaacctc gccggtcgat tcgagccagt tctcggcgaa ggtgccctga
     2281 aggctcgcca cgccgtcgcc ctcgacgcgg aacatcgtgt cgcgccagcg cgggtgccct
     2341 tcgcgcggga tgagccagtg gtcggcgaag ccggcgccgc cgatgaagcc gacgcggccg
     2401 tcaacgatga tcagctcgcg gtgcgtgcgg ttgttgaagc gggcgatggt ctgccagcgc
     2461 ggcgggtgat accagccgac gcgcccgccc gcctcggtca gctcacggaa gtagctcgtc
     2521 caggaggtga aactcccgag ccagtcgagc acgaggcgca cctcgacgcc ggcgcgcgcc
     2581 cgctccgtga ggacttcgag gaagcgacgc gtgacctcgc ccttctggaa gatgtaagcc
     2641 tcaaggttga tgctgctcgc ggcggcgcgc aacgcctgaa gctcggcctc gtagtagacc
     2701 tcgccgttgg tcaacacctc gacgcgcgtg cagtcgccca cgcgcgagtc ggtgatggat
     2761 tcgagcatcc gcaggaagtc gggcgagtcg agcggggccg cgtagggcga agagattttg
     2821 tagtcgaggc ccggctcgaa gagcgccagg aagagcagga agctcaacac gacgatggcg
     2881 atgatggcga cgacgttgaa ccagtctatc tgcaagtggc gaccctcagc cctgaccttt
     2941 gactcgaacc gaccccgtcg gagacggcgc tcaaacgaga cccagcaact cgccgacgtt
     3001 catctcgaag cctgagagca ccgcgcttct gatggcgtcc gcctcaacgt gctccgtcag
     3061 cgcgtagtgc tttttgcgga ggacgagctg cgagatggtg cgcgccgccg gatcgacaat
     3121 ccaatattcg cgcacgccgt ttcgctcgta gagatttctc ttcctcaaag tgtcgtgcgt
     3181 aatcgagccg ggcgagataa tttcgacgag cagcggcggg gtggcgtgca cgcgctttgc
     3241 cgccaacgcg cgtttagcat cccgactcgt gaggaaaacg acgtccggct gaacgacgtc
     3301 cccgcttggc agaacgacat cgacagggga gaggaagatc gtgccgagtg aattgttccg
     3361 cgcgaaatta cccagggcgg tgaagatgtt gccaagcacg aactggtgca gcgggacggg
     3421 tgctggagcc atgaggattt ctccattgat gtactcggtg agaagtactg attcgggcag
     3481 cgcccaaaat tcttcgatgg atgccgcccg gtgcgtgagg cgctgctcgg tggtcggttc
     3541 taaaacttcg gtcgccatgt ctttctccct cttgacggtc ggaggttcct cgtcggctaa
     3601 aattttatca cggcgcgggc gagcgcgtag acggaacttc gctggcgtag cgggcgcgga
     3661 cgcggacgca gcgttcgagc gcgtcggcgg cttcgagcac tttgaggcgg aggtcgcggt
     3721 cgagccgggg ttcgcggcgg aggaagtcgt tgaccgtgtc gagcgcagcg gggctgcatt
     3781 ggccgccgag gaaggcggcg agccagccgt tgacgaagaa gattttgcgc gtgcgcttga
     3841 gcgtggggag ctggcggagc gcgggcgcga ggtaggggag cgtcagttcc gactgccgcg
     3901 gcgtgttgaa ggggccgacg gcggcctcga tccagctttc ggccagcgcc gggtcgttca
     3961 ggaagtcgtc gaagtacttg ttcttgactg acgcgacggg ccgcgccgcc tcggccgcgt
     4021 aggcgtagcg ccgcccgtcg tcgctcgcgt cctgactgac gacgctcttg aatagtgcct
     4081 cggcgcgctc gtcgcctcct cctccgcggc gtgagagcag tgagcgcgcg atgtcgaagc
     4141 ggtcgcgcga gcgcaaggtc atgccgggca cctgtagcgc gcccgtgagg atgctctcca
     4201 gcttgtcgaa ggcggcgggc gtagtcgcca cctcgcggaa ggttcggtag taggtgatgc
     4261 gaagccccac tgtttcggcc gtcaacatct gcgacgagag ccacgcttcg agacgcgggg
     4321 cgacctgcgc ttgctgctcg ccggagaggt aacgcgcgta ggccgtctgc acgcgcgcga
     4381 gtacggactg gagcgtcacg tcgtcgcgct cgcggcccgc gtgtcggacg gcgaggtcga
     4441 tgtattcgag cggcccggtc tcggcctcgc gcacgccctc ccagagcgcg ccccagagca
     4501 gcgtgcgcag gaactcgtcg tcgatggcgc cgaaattctt gagcaccgcc gcgcggctcg
     4561 cgtcgtcgag caggaagcgg ccgtagccgt agtcctcgtg attggcgaag acgaacgacg
     4621 ggcggcgacg gccgacggcc tcccgaactt ccgtcacgcc gtcgccgctc aaggtcacgt
     4681 cgagcgtctc ggggcgacgc cccgagcgcg ccggggcgag caggagtttg acgcgcatcg
     4741 gccacgcgcc gccctcgccg agcacgtccc gttgctcgac ggtgagacgg ctgatgcggc
     4801 cacgtttgtc ggccgaccac ttcacgcgca cgtcgggcat cccgcggcgc ttgacccacg
     4861 agtcggccca aagtgagagc ttgcggcccg aagcgcgctc gaaagcgccg acgaggtcgg
     4921 cccactcggc gttcgcgtag gcgtgttctt tgacgagcag gcggatggcg cggcggaagg
     4981 c
//
LOCUS       DASVES010000248        12820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_68607, whole genome shotgun sequence.
ACCESSION   DASVES010000248 DASVES010000000
VERSION     DASVES010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68607"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..435)
                     /locus_tag="VF240_10145"
     CDS             complement(220..435)
                     /locus_tag="VF240_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175607.1"
                     /translation="MSEERNEPRIRLYDLRHSCVTLLLSAEENPKVVSERLGHSTIVL
                     TLDTYSHVLPTMHQKDSENLERMLYGG"
     gene            510..929
                     /locus_tag="VF240_10150"
     CDS             510..929
                     /locus_tag="VF240_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175608.1"
                     /translation="MKRMRVKSIACLLLCGVTLLAVSPSKAFAQTGGRPAVARGGEDL
                     SDGRAKSEPRLKEIFAGEVVKDRAGVMSEADIKRLEKESLFPQSAPNSHGFSKRDTFL
                     VVVIVVVIVGLAIVLEHNGVKSVVRCEDDPFAPDCVQ"
     gene            1088..1654
                     /locus_tag="VF240_10155"
     CDS             1088..1654
                     /locus_tag="VF240_10155"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7175609.1"
                     /translation="MDKAVFTTIESSRLILRRFADADLHTFLAYLNDPLVARYQTWES
                     YTEQQAKDVIDQQQGLEAGRPGGWFTFAVELKEIKSLIGHVALSVKEADHRQAEIGFT
                     FAREYQGRGLAREAAECVLDYAFNELGMHRIVAITDCRNVRSVALLSRLGMRREGHFI
                     ENVWFKGGWGSEYVYALLRKEWLSRRPS"
     gene            2394..4949
                     /locus_tag="VF240_10160"
     CDS             2394..4949
                     /locus_tag="VF240_10160"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX7175610.1"
                     /translation="MTRIILVLFVCLLLIPSDSLAQERKVPKSIADATKGLQKIDGFV
                     PLYWDAEGGRMLLEVGRWNSEMLYQISLPTGVGSNPIGLDRGQLGDTHVVFFERAGNK
                     VLLTEPNYRFRALTDNAAERRAVEESFARSVIWGFKVEATEGERVLVDATSFFMRDAH
                     GVADRLREAEQGGFNLDESRSAFHMPRTKGFPKNTEVETTLTFTSSQPGQLVRETVPA
                     PRAVTVRQRHSFVELPDSGYRPRRLDPRVGVFAVDFYDYATPISEPLEKRWIVRHRLE
                     KKDPSAASSEAVRPIVYYVDQGTPEPIRGALIEGAAWWNAAFEAAGFRDAFQVKMLPA
                     DADPMDVRYNMINWVHRSTRGWAYGSTVVDPRTGEVVRGVVTLDSQRARQDYLLGSGL
                     VPPHPLGGASASAARSGGCDFALLPELDYLIQADSPETLTAMAVARIRQLSAHEVGHT
                     LGLAHNFAASTYGRASVMDYPAPLVEIKNGQLDLSNAYAVGIGSYDKFAIRYAYTQFA
                     AGTEETAELEKIVREGVANGMLFLSDSDARPPGAAHPLANLWDNGADPVAMLEHTLEV
                     RRIGLKQFGPANIPRGAPLSTLEAKLLPLYLHHRYQLQAAIKSIGGVYYTYAVKTPDG
                     HNPARVYEIVPAGQQMKALYAAIRALRSDELRMPQHVLDMIPPRAFGYEGGTAEMFPK
                     RTDPVFDHIGAATLAADLVLTDLLEPHRASRMIQFNAQQSSYPHFDETIWHVVQVTWK
                     VSYPGDRFANATARAVQSLTVTRLMDLAANQDASPQVRAAAVKGLRDIAEYIDYNTKG
                     RTATVREQDVLEHMRYTRDDIERFLTRPDEPRRRTRPLPRPPGDPIGSGPQSP"
     gene            5205..5435
                     /locus_tag="VF240_10165"
     CDS             5205..5435
                     /locus_tag="VF240_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175611.1"
                     /translation="MATKKSAKKSGAKKSAKKAAAKKSGAKKAGISLPGLPPNFACIS
                     SCLIKYRLCVKTATDKVSCQKKLIACIDKCLL"
     gene            5641..7032
                     /locus_tag="VF240_10170"
     CDS             5641..7032
                     /locus_tag="VF240_10170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7175612.1"
                     /translation="MKILILCALMLCLPLLGMAQQPKGQPKARKLFPYDYTIDDLPNG
                     LRLVTVPTDYPNLVALYIVVQAGSRNEVEEGKTGFAHFFEHMMFRGSENYTAEQRDAL
                     LKRAGAEANAYTSDDRTVYHETFAKEDLDAVMKLEADRFMRLKYALPEFQTEAKAVQG
                     EYDKNSANPFSKLYEVLRETAFGKHTYSHTTMGYLKDIKEMPNQFDYSREFFKRFYRP
                     EYTTIVLVGDVTRARSLELTKKYFGAWERGSYKADIAAEPPQKGAREKHIDWPSPTLP
                     VVAVAFRGPAYSDVEKDKAALDLLAQVAFGETSEIYQRLVLREQKVDILGPDFSNQID
                     PELFVVLARVKDVKDVPGVRDQIIAAFRRYTTEPIPQQKLDATRSRMRYSLAMAMDSS
                     DSIASLLAPFISLRRTPETIDKYAALLDTLTPVDVRDTAAKYFKDESRTVVTLATKTG
                     ADAARNESKGGDN"
     gene            7086..8558
                     /locus_tag="VF240_10175"
     CDS             7086..8558
                     /locus_tag="VF240_10175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7175613.1"
                     /translation="MNTANAQRSPATATGGDPANFVLLPSRSPLVTFRLLLMTGAASD
                     PAGKEGVASLTAAMLAEGGSRARTYEQVVNEMYPMATSFGWQADKEMTVFTGATHIDN
                     LEKYYGLIREMLLDPGFREEDFTRLRTNAVNFLKVSLRETNDEELGKEYLYNVIYKGH
                     PYEHHNMGRVSTLERLTLDDVRAFYKANYTRANLVVGLSGGYPADFPARLKADFQKLP
                     EGAAKTAKYATPRLDAGMKIDIIQRETRATALSLGFPLSVNRAHKDYAALALVASYFG
                     QHRSSNSYLYQRLRETRGLNYGDYAYIEYFPRGMFGFTPDPNLGRSTQIFQIWIRPVE
                     PQNGHFVLRAALYEYDRLVRDGMTQQDFEATRDFLIKFNNILTQTQDAQLGYALDSRY
                     YQMGDYVTHMREQLQRLSLADVNRVIREHLKSESMRVVVITKDAEALRDAILKSTPSP
                     IKYNSEKPAELLREDKVIETYKVSVKPEDVRIVPVAQVFE"
     gene            complement(8858..9610)
                     /locus_tag="VF240_10180"
     CDS             complement(8858..9610)
                     /locus_tag="VF240_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175614.1"
                     /translation="MQNQGIPPPSSFGAPQPPAKKGLSKGCIIGIIAAVVVAVGGVIL
                     IAAGLGGYFLYRSSETENVGTKTSVGVGPVKTSTKTSGAPAAEGPNPTDAQRAAVSGG
                     QTAEWTQQEISWTVPQRWSRYSEDSTSLLWRSPGSWDAASLIVSISPMDASFPVEASI
                     NAFYEQAQTRKTNGEVNEVRWLMLDGIKGVLFRESSPESDDNPQRLQWMAYRNYKGQV
                     QLVNIMLASRGKDFARHEDVLYGILYSTEFME"
     gene            9823..11268
                     /locus_tag="VF240_10185"
     CDS             9823..11268
                     /locus_tag="VF240_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175615.1"
                     /translation="MQTSLLPEHLGALIDRLREANSEFAARFPGETGRRQPVHTVYGG
                     AHLFKADTARRLGSLALRALEEYGSDAFVFARAVGLPGSDELNSTLEKDADLRARLET
                     EPELVRRVAPTAWMAHTVHARVIEKLKREPVEDFRIDFEDGYGNRPDEEEDGHAAQAA
                     REVTAGLAAGTLPPFIGIRIKPFTEELRERSFRTLDIFVTTLAAEAGGRLPENFVVTL
                     PKVVAVEQVAALADIFDLLEPALGLAAGTLRMELMVETTQSIINQRGEANLPLLLDAA
                     RGRCTAAHFGTYDYTASCQITAAHQHMLHPSCDFARHMMQIAFAGTGVWLSDGATNIM
                     PVAVHRPAEGQTLTEEQTEENHAAVHRAWALHFSHVRNSLTNAFYQGWDLHPAQLPTR
                     YAAVYSFFLESLAAASERLSNFVEKAAKATLVGDVFDDAATGQGLLNYFLRAINCGAV
                     TEREAQELSSLTLEELRTGSFVKILKGRQNL"
     gene            complement(11607..12422)
                     /locus_tag="VF240_10190"
     CDS             complement(11607..12422)
                     /locus_tag="VF240_10190"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX7175616.1"
                     /translation="MRTNPLVMEWINLAARWAHVFAAILWVGSTYFFTWLDGRFEEAA
                     RKSEGKSGEVWMVHSGGFYVVEKQKGLERLPPALHWFRWEAATTWLTGMALLVVVYYM
                     GGALVDRDVWDVSAGVASLASLGVLATSGLVYDLLMKSPVGRNEKAFAVVAFALVVAA
                     SYLLTRLFSARAAYIHLGAIFGTIMVANVWMHILPAQSRMVAALKEGRPADESHGARA
                     KLRSKHNTFMAVPVVFTMISHHFTSVYGHEYNWVILSALVLVGWVAAKLIRRA"
     gene            complement(12431..>12820)
                     /gene="uraH"
                     /locus_tag="VF240_10195"
     CDS             complement(12431..>12820)
                     /gene="uraH"
                     /locus_tag="VF240_10195"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX7175617.1"
                     /translation="FVLSLITHHLSLPNDMSAITTHVLDTSRGRPAAGVAVLLEVQAE
                     GGAWRMLGRGETDSDGRLRTLLAPDAEPRAGVYRLTFDTGAYFAAQGVESFYPAAAIV
                     FAVRDGGEHYHVPLLLSPFGYSTYRGS"
BASE COUNT         2486 a         4276 c         3813 g         2245 t
ORIGIN      
        1 ggctgagttc gactatgcct cagacgccga caaatacgcc gagggacaag gcggcaaagg
       61 cgctgactgc aagtgcccgt aaggcttgtg aataacccca tcaaaagctc atcagaccca
      121 accatgaccg tcctctttag aaggtcgagt catgggctag cattacggca aaggttattc
      181 caagtgaggt tatgcgccaa ctgcggtgta ttcataatgc taacctccgt agagcattct
      241 ctccagattc tccgaatcct tctggtgcat tgtcggtagc acatgactgt acgtgtcgag
      301 cgtgaggacg atggtcgagt gaccgagacg ctcgctgacg accttcggat tttcttccgc
      361 gctcaacaga agggtgacgc atgagtggcg caggtcatag agcctgatgc gcggctcgtt
      421 cctctcctct gacatcaaac ctgccttttt taccacgtgg atttataaac ttagtgcaac
      481 aggcttccct gaagcggagg tgacgggggt tgaagagaat gagagtcaag tcaatcgctt
      541 gcctgttgct ttgtggcgtc acgctcttag cagtttcgcc gtcgaaagcc ttcgcccaaa
      601 cgggaggccg acccgccgtc gcccggggag gagaagatct gagcgacggg cgggcaaagt
      661 cagagccccg actgaaggag attttcgccg gtgaggtggt gaaggacaga gccggtgtca
      721 tgtcggaggc ggacatcaag cggcttgaga aagagagtct gttcccgcaa tccgccccga
      781 actctcacgg gttttccaag agggacacat ttctggtggt cgtcatcgtg gtggtgattg
      841 tgggactggc gattgtgttg gagcacaacg gggtgaagtc ggtagtgcgt tgcgaagatg
      901 atcctttcgc tcccgattgt gtccagtgac cggaggcgcc gcgcaccgtc gggggaagta
      961 actcatcgcc gcgcctgaca acggcctgcg cccgacgccc catcacggcg acactcatac
     1021 acgctgcaca ggggcgcgag tgatgatggg cgttagatgg cttcggttgt cgtcagtaat
     1081 gacctgtatg gataaagcgg tattcaccac catcgagtct tcaaggttga ttctccgccg
     1141 cttcgccgat gccgaccttc acacgtttct tgcatatctc aatgacccgc tcgtggctcg
     1201 ctaccaaacc tgggaatcat ataccgaaca gcaggccaag gatgtgatcg accagcagca
     1261 gggcctcgaa gcaggccggc cgggggggtg gttcaccttc gccgtcgagc tgaaagagat
     1321 aaaatctcta atcggtcacg tcgccctcag cgtgaaggaa gccgaccacc ggcaggcgga
     1381 gatcggcttt acgtttgccc gcgagtatca gggcagaggc ttggccaggg aggcggcgga
     1441 atgtgtgctg gattacgcct tcaatgagct ggggatgcac cgtattgtgg cgatcacgga
     1501 ttgtcggaat gtgcgctcgg tggcgctgtt gagcaggctg gggatgaggc gggaggggca
     1561 cttcatagag aacgtctggt ttaagggcgg gtggggatcg gagtatgtgt atgccctgct
     1621 gcgaaaggag tggttatcga ggcggcccag ctaacgacgg catgcaccgg aacgcgaatc
     1681 agcgcggctg tcatcgataa cttgcgtggt ttcgaggttg gtcaggtgtt agaagtcgat
     1741 ggtcaactta gaaacgcggc atctcgtccg acggaggtgg tgcgtcctcc agacagacct
     1801 cgccgccgac ggctgctatt gagccgcccc gcgccggcct caatcacgtc caactcttat
     1861 gcgaggatgt tgtggcgcga gtgcgtgtta ttgctcgcag acggtcgcat agcaatcaac
     1921 aatacaactg ttgaacccgt cgtggcactg ctgttgctcc tccaccggat atctgttcag
     1981 acacctatcg tagtccctct ggcatctacc ttgacatctg acgcatgggt cgggcctctg
     2041 gacagctctt accgtgcggc tggtagaaag cgaagggaaa gtgagcgcca gcatgaagac
     2101 gatcaatgcc gcgcagaaaa caaatgacct tctcatagta gactccttcg gatttagagt
     2161 ggggcgagga ctcgtcgaga tgggatgttc ctataacaga ttccactgga agtcaatcac
     2221 accctgcgtc gggtagtgtc ctaaattgaa tgtttcctca gcgcgtcctc ggcgtcctcc
     2281 gcgcctcggc ggtgaatcaa ggctgaaggc cactcaccgc cgagtcgccg aggatgcgct
     2341 gagaactgaa attacgacac tattagaaaa cagcggcctg gaagatcatc actatgacac
     2401 gaatcattct tgtccttttc gtctgcctgc tcctcattcc ttctgactcg ctcgcacagg
     2461 agcggaaagt cccgaagtcc atcgcggacg caacgaaggg tttgcaaaag attgacgggt
     2521 tcgtgccgct ctactgggac gccgagggcg ggcgaatgct gctggaggtc gggcgttgga
     2581 actcggaaat gctttatcag atttcgctcc cgaccggcgt gggttccaac cccatcggcc
     2641 tcgaccgcgg acaactcggc gacacgcacg tcgtcttctt cgagcgcgcc ggcaacaagg
     2701 tgttgctgac ggagccgaac taccgcttcc gcgccctcac cgacaacgcg gccgagcgac
     2761 gggccgtcga ggagtcgttc gcgcgctccg tgatctgggg cttcaaggtc gaggcgacgg
     2821 agggcgagcg cgtgctcgtg gacgcgacct cgttcttcat gcgcgacgcg cacggcgtcg
     2881 ccgaccgcct gcgcgaggcc gagcagggcg gcttcaatct cgacgagtcg cgcagcgcct
     2941 tccacatgcc ccgcacgaag ggctttccca aaaacacgga ggtggagacc acgctcacct
     3001 tcacgtcgag ccagcccgga caactcgtgc gcgagaccgt gcccgccccg cgcgccgtca
     3061 ccgtccgcca gcgccactcc ttcgtcgagc tgcccgactc cggctaccgc ccgcgccgac
     3121 tcgacccgcg cgtcggcgtc ttcgccgtcg atttctacga ctacgcgacg cccatcagcg
     3181 agccgctcga aaagcgttgg atagttcgtc accggctgga gaagaaagac ccgtcggcgg
     3241 ccagttccga ggccgtgcgg ccgattgtct actacgtcga tcaggggacg cccgagccga
     3301 ttcgcggcgc gctcatcgag ggcgccgcgt ggtggaacgc ggcgttcgag gcggccggct
     3361 tccgcgacgc ctttcaggtg aagatgctcc cggccgacgc cgacccgatg gacgtgcgct
     3421 acaacatgat caactgggta caccgctcga cgcgcggttg ggcctacggc agcacggtcg
     3481 tcgatccgcg cacgggcgag gtcgtgcgcg gcgtcgtcac cctcgactcg cagcgcgccc
     3541 gacaggatta tctactcggc tccggcctcg tcccgcctca tcctctcggc ggggcatctg
     3601 cttcggccgc gcgctccggc ggctgcgact tcgccctgct ccccgaactc gactatctca
     3661 ttcaagccga ctcgcccgag acgctgacgg cgatggccgt cgcccgcatc cgacagctct
     3721 cggcccacga ggtcggccac acgctcggac tggcgcacaa cttcgccgcc agcacctacg
     3781 gccgcgcctc cgtcatggac taccccgccc cgctcgtcga gattaaaaac ggccagctcg
     3841 atttgtcgaa cgcctacgcc gtcggcatcg gctcatacga caagttcgcc atccgctacg
     3901 cctacacgca gtttgcggcg ggcacggagg agacggcgga gcttgaaaag atcgtccgcg
     3961 aaggcgtcgc taacggcatg ctcttcctct cggactcgga cgcgcgcccg cccggcgccg
     4021 cgcatccgct cgcgaacctc tgggacaacg gcgccgaccc cgtcgcgatg ctcgaacaca
     4081 ctttggaggt gcggcgcatc ggcctcaagc agttcggccc cgccaacatc ccgcgcggcg
     4141 ccccgctctc cacgcttgag gccaagctcc tcccacttta tctgcatcac cgctaccagc
     4201 ttcaggccgc catcaaatcc atcggcggcg tctattacac ctatgctgtc aaaacgcctg
     4261 acggccacaa ccccgcgcgc gtctacgaaa tcgtccccgc cggacaacaa atgaaggcgc
     4321 tttacgccgc catccgcgcg ctccgctccg acgagttgcg gatgccgcag cacgtcctcg
     4381 acatgattcc gccgcgcgcc ttcggctacg agggcggcac ggccgagatg ttccccaaac
     4441 gcaccgaccc cgtcttcgac cacatcggcg cggccacact cgccgccgac ctcgtcctca
     4501 cagacctgct tgagccgcac cgcgcctccc gcatgatcca gttcaacgcg cagcagtcct
     4561 cttacccgca cttcgacgag accatctggc acgtcgttca ggtcacgtgg aaagtctcct
     4621 accccggcga ccgcttcgcc aacgccacag cgcgcgccgt gcagagtctc accgtcacgc
     4681 gcctgatgga cctggcggcg aatcaggacg cctcgccgca ggtgcgcgcg gcggccgtca
     4741 agggcctgcg cgacatcgcc gagtacatcg actacaacac gaagggccgc accgcgaccg
     4801 tcagggagca ggacgtttta gaacacatgc gctacacgcg cgacgacatc gaacgcttcc
     4861 tcacgcgccc cgacgaaccg cgccgccgca cgcgcccgct gccgcgcccg cccggcgacc
     4921 ccatcggctc cggcccacag tcaccctaac cgtgaagtct ctacggccga gcgaacaccg
     4981 ttcgcgcctg aagtcattca gtgagagcca accggcgcgc gaattaattc gttgacccgc
     5041 cgccccatct gtcatatctt cgccgcgccc gcaccgttgg cgagtcttca cgagcatggg
     5101 ccgtcgtcgt tccctttgac ctcacggggg gtcttctaca gagatcgcgc gccgcggcgt
     5161 ttccacgcgg cgccccacct gacgatgaag gagaccgaaa gaaaatggcc acgaagaaga
     5221 gcgcaaagaa gagcggcgcc aagaagagcg ccaagaaagc cgcagccaaa aagagcggcg
     5281 ccaagaaggc cggcatctct ttgcctgggc ttccgccgaa cttcgcctgc atcagttcct
     5341 gcctcatcaa gtatcgccta tgcgttaaga cggccactga caaggtgagc tgccagaaga
     5401 agctgatcgc ctgcatcgac aagtgtcttc tctagtttat ccagaaccgg aagcgcgaga
     5461 catcagttcg gcgctttgaa aatgcggccc gccccgagtc atgattggag actccggcgg
     5521 gccgcacttt cgttgacgtg tgaaggggct ggcggcttcg ccaactcaat ttccgtcggt
     5581 tataatgcca acagtccaca ttcaaagcag ccctgtcacc acgttttagg agaccggaag
     5641 atgaaaatat taatcctttg tgccctcatg ctctgcctgc cgctcctcgg catggcccag
     5701 cagccgaaag gccagccgaa ggcgcgcaaa ctctttccat acgactacac catcgacgat
     5761 ttgcccaacg gcctgcggct cgtcaccgtg ccgaccgact acccgaacct cgtcgcgctc
     5821 tacatcgtcg ttcaggccgg ctcgcgcaac gaggtcgagg agggcaagac cggcttcgca
     5881 cacttcttcg agcacatgat gtttcgcggc agtgagaact acacggccga gcagcgcgac
     5941 gccctgctca agcgcgccgg cgccgaggcc aatgcctata cctccgacga ccgcaccgtc
     6001 taccatgaga ccttcgccaa ggaagacctc gacgcggtga tgaagctcga agccgaccgc
     6061 ttcatgcgtc tcaagtacgc gctgcctgag ttccagaccg aggccaaggc cgttcagggc
     6121 gagtacgaca agaactcggc caaccccttc agcaagctct acgaggtgct gcgcgagacg
     6181 gccttcggca agcacaccta ctcgcatacg acgatgggct atctcaagga catcaaggag
     6241 atgcccaacc agttcgacta cagccgcgag ttcttcaaac gcttctaccg tcccgagtac
     6301 acgaccatcg tcttggtcgg cgacgtgacg cgcgcgcgct ctttggagtt gacgaagaag
     6361 tatttcggcg cgtgggagcg cggcagctac aaggccgaca tcgcggccga gccgccgcag
     6421 aagggcgccc gcgagaagca catcgactgg ccctcgccca cgcttcccgt ggtcgccgtc
     6481 gccttccgcg gccccgccta ctccgacgtg gaaaaagata aggccgccct cgatctgctc
     6541 gcgcaggtgg ccttcggcga gacctcggaa atctatcagc ggctcgtcct ccgagagcag
     6601 aaggtggaca tcttaggccc cgacttctcg aaccagatcg accccgagtt gttcgtcgtc
     6661 ctcgcccgcg tcaaggacgt gaaggacgtg cccggcgtcc gcgatcagat catcgccgcc
     6721 ttccgacgct acaccaccga acccatcccg cagcagaagc tcgacgccac gcgctcgcgc
     6781 atgcgctaca gcctcgccat ggcgatggac agctccgact ccatcgccag cttgctggcc
     6841 ccgttcatct ccctgcgccg cacgcccgag accatcgaca agtacgctgc cctgctcgac
     6901 acgctcacgc ccgtggacgt gcgtgacacg gcggccaaat acttcaagga cgaaagccgc
     6961 accgtcgtca cgctggcgac caagaccggc gctgatgcgg ccaggaacga atcgaagggg
     7021 ggcgacaact gatgcgacga cgcacactca cggccctgat cctgtcactt gcgacactcc
     7081 tgtctatgaa cactgcaaac gctcagcggt cgcccgccac cgcgacgggc ggcgaccccg
     7141 ccaacttcgt gctgctcccg agccgctcgc cgctcgtcac cttccgcctg ctcttgatga
     7201 ccggcgcggc ctccgacccc gccggtaagg agggcgtggc ctcgctcacc gccgccatgc
     7261 tggccgaggg cggctcgcgc gcgcgcacct acgagcaggt cgtcaacgag atgtacccga
     7321 tggccacctc cttcggctgg caggccgata aggagatgac cgtcttcacg ggcgccaccc
     7381 acatcgataa cctggaaaag tactacggcc tcatccgcga gatgctcctc gaccccggct
     7441 tccgcgagga ggacttcacg cgccttcgca ccaacgccgt caacttcctc aaggtcagcc
     7501 tgcgcgagac gaacgacgag gagctgggca aagagtatct ctacaacgtc atctacaagg
     7561 gccaccccta cgagcatcac aacatgggcc gcgtcagcac gcttgagcga ctgacgctcg
     7621 acgacgtgcg cgccttctac aaggctaact acacgcgggc caacctcgtc gtcggcctct
     7681 cgggcggcta cccggccgac ttccccgcgc gcctcaaggc cgacttccag aagttgcccg
     7741 agggcgcggc gaagacggcg aagtacgcga cgccgcgcct cgacgccggc atgaagatcg
     7801 acatcatcca gcgcgagacg cgcgccacgg ccctctcttt aggcttcccc ttgagcgtca
     7861 accgcgcgca caaggactac gccgccctcg cgctcgtcgc cagctatttc ggccagcacc
     7921 gctcctcgaa cagctatctc taccagcgcc tgcgcgagac gcgcgggttg aattacggcg
     7981 actacgccta catcgaatac ttcccacgcg ggatgttcgg attcacaccc gacccgaacc
     8041 tcggacgctc gacccagatt ttccaaatct ggatcaggcc cgtcgagccg cagaacggcc
     8101 acttcgtcct gcgggccgcg ctctacgagt acgaccggct ggtgcgcgac ggcatgacgc
     8161 agcaggattt cgaggccacg cgcgacttcc tcatcaagtt caacaacatc ctcacccaga
     8221 cgcaggacgc gcagctcggc tacgccctcg acagtcgcta ttaccagatg ggcgactacg
     8281 tcacgcacat gcgcgagcag cttcagcgcc tctcgctggc ggacgtgaac cgcgtcatcc
     8341 gggagcacct taagtccgag tcgatgcgcg tcgtcgtcat cacgaaagac gccgaggctc
     8401 tgcgcgacgc gatactcaag agcacgccgt cgcccatcaa gtacaactcc gagaagccgg
     8461 cggagcttct gcgcgaggac aaggtgattg agacctacaa agtgagcgtc aagcccgagg
     8521 acgtgcggat cgtccccgtg gcgcaggttt tcgagtagga gccgcaagcg cctacctctt
     8581 ccatcagccg cgcaagcggc ggcagaatgt agcccacggc gtgagccgtg gggacgagcc
     8641 ggataaaatt aaggaagccc cggaggggcg aaagaacctc aacgcgcgcg tgatgccgtc
     8701 tgaggttctt tcgcccctcc ggggcttcct tgttttcgtt gtcgttacca cgggcttacg
     8761 cccgtggcta caatctgccg ccgctccgcg gctggttgaa attcttctct acgacccgac
     8821 tcaggcgcgc cccggcatgt tacctatggt cgtgctgtta ctccatgaac tccgtcgagt
     8881 aaaggatgcc gtagagaaca tcttcgtggc gggcgaagtc tttaccgcgc gaggcgagca
     8941 tgatgttgac gagctgaacc tgccccttgt agttgcggta ggccatccac tggaggcgct
     9001 gcggattgtc gtccgactcc ggcgaggact cgcggaacaa cacgcccttg atgccgtcaa
     9061 gcatcagcca gcgcacctcg ttgacctcgc cgttcgtctt gcgcgtctgc gcctgctcat
     9121 agaaggcgtt gatgctcgct tccacgggga agctcgcgtc catcggcgag atcgagacga
     9181 tgaggctggc cgcgtcccac gaacccggcg agcgccagag cagagaggtc gagtcctcgc
     9241 tgtacctgct ccaacgctgc ggcaccgtcc acgaaatctc ctgctgcgtc cactcggccg
     9301 tctgaccgcc agagaccgcg gccctttgcg cgtcggtcgg attcggtccc tccgccgccg
     9361 gggcgcctga ggtcttcgtg gaagtcttga cggggccgac gcccacgctg gtctttgtgc
     9421 cgacgttctc cgtttcactg cttctgtaca ggaagtagcc gccgaggccg gcggcgatga
     9481 ggatgacgcc cccgaccgcc acgacgacgg ccgcgatgat gccgatgatg cagcccttgg
     9541 aaagcccttt cttcgcgggc ggctgcggag cgccgaagga cgatggcggg ggaatgcctt
     9601 gattttgcat ggtgttgacg aagctccttc cgggggttta atgacgagcg aggagcgcgg
     9661 actatatcac gaacccgccg aggtttatta gaatcaaatt aggcggaagc ggcggccgtc
     9721 cccgtgggcg atccgcccac accgcgctcg cgcccggctg acagtctccg ctagaatgcg
     9781 actcgttcaa acaatcatcc gacaccagac cgaaggagcg acatgcaaac ttccctctta
     9841 ccggaacatt tgggcgcgct aatcgacagg ctgcgtgagg ccaattccga attcgccgcg
     9901 cgcttccccg gcgagacggg ccgccgccag cccgtccaca ccgtctacgg cggcgctcat
     9961 ctgttcaagg ccgacaccgc gcgacgcctc ggctcgctcg cgctccgcgc cctcgaagag
    10021 tacggctcgg acgccttcgt cttcgctcgc gccgtcggac ttcccggctc tgacgaactc
    10081 aacagtacgc ttgagaagga tgccgacctg cgcgcgcggc tggagacgga gccggaactc
    10141 gtgcgccgcg tcgcccccac ggcttggatg gcccacaccg tccacgcgcg cgtcatcgag
    10201 aaactcaagc gtgagccggt tgaggatttc cgcatcgact tcgaggacgg ctacggcaat
    10261 cgccccgacg aagaagaaga cggccacgcc gcgcaagccg cgcgcgaggt gaccgccggc
    10321 ctggccgccg gcacgctccc tccgttcatc ggcatccgca tcaagccctt caccgaggaa
    10381 ttgcgcgagc gcagtttccg cacgctcgac atcttcgtca cgacactcgc cgcggaggcc
    10441 ggaggccgtt tacctgagaa cttcgtcgtc acgctgccga aggtcgtggc ggtcgaacag
    10501 gtggcggcgc tcgccgacat cttcgatctg ctggagccgg cgctcggcct cgcggccggt
    10561 acgctccgca tggagttgat ggtcgagacc acgcagtcga tcatcaacca acggggcgag
    10621 gcgaacctgc cgctcctgct agacgcggct cggggtcgtt gcacagccgc gcacttcggc
    10681 acctacgact acacggcaag ctgccagatc accgccgccc accagcacat gctccacccg
    10741 tcttgcgact tcgccaggca catgatgcag atcgccttcg ccggcaccgg cgtctggctc
    10801 tccgacggcg cgaccaacat catgcccgtc gccgtccatc gcccggccga gggtcagacg
    10861 ctcacggagg agcagacgga ggagaaccac gcggccgtcc accgcgcctg ggcgctccac
    10921 ttctcgcacg tccgcaactc gctcacgaac gccttctatc aaggctggga ccttcacccc
    10981 gcgcagctcc ccacacgcta cgccgccgtc tattccttct tcctcgaaag cctcgccgcc
    11041 gcctccgagc gcctgagcaa cttcgtcgag aaggcggcga aggccacact cgtcggcgac
    11101 gtgttcgacg acgcggccac tggtcagggc ctcctcaact atttcctccg cgccatcaac
    11161 tgcggcgctg tcaccgagcg cgaagcgcag gagctgagca gcctgacgct cgaagaacta
    11221 cgcacgggat cgttcgtcaa gattctgaag ggtcggcaaa acctctaaag aaggcaaagg
    11281 taaaaaggca aaaggcaaaa gtgagatagc ggaatcgcag gcgtcaatga gccgcggagc
    11341 ggcggaagat tgtagccccg gtcgtgagac cgggatatgc ggtttagaaa aattcaatga
    11401 agccccggag gggcgaaaga aatgcgacgc gcgcaccaag ccaacgtcgc atttctttcg
    11461 cccctccggg gcttcggttc attaattgga actgtcccca cggctcacgc cgtgggcaac
    11521 attattccgc cgcctccggc ggctggttga cgcccgtgtt tggcttccgc acttttgcct
    11581 tttgcctttt tacttttgcc ttgacctcac gctcgcctga tgagcttcgc cgcgacccag
    11641 ccgacaagaa ctaacgcgga gaggatcacc cagttgtact cgtggccgta gacggaggtg
    11701 aagtgatggc tgatcatcgt gaagacgacg gggacggcca tgaaggtgtt gtgcttcgag
    11761 cgcagcttgg cgcgcgcgcc gtgcgactcg tcggcggggc ggccttcctt cagtgcggcg
    11821 accatccggc tctgcgccgg caggatgtgc atccagacgt tggccaccat gatggtgccg
    11881 aagatggctc cgaggtggat gtaggccgcg cgcgcgctga acaggcgcgt cagcaggtag
    11941 gaggcggcga cgacgagcgc gaaggcgacg accgcaaacg ccttttcgtt tctgccgacg
    12001 ggcgacttca tcaacaggtc gtagaccaga cccgaggtgg cgaggacgcc gaggctcgcg
    12061 agggaagcca cacccgcgct cacgtcccac acgtcgcggt caacgagcgc gccgcccatg
    12121 taatagacga cgacgagcag cgccatgccg gtcagccacg tcgtcgccgc ttcccagcgg
    12181 aaccagtgaa gcgcgggcgg caggcgctcc agccccttct gtttctcgac gacgtagaag
    12241 ccgccgctgt gaaccatcca gacctcgccg gacttgccct cggatttgcg cgcggcttcc
    12301 tcgaagcggc cgtcgagcca ggtgaaaaaa tatgtggagc cgacccagag gatggcggcg
    12361 aagacgtgcg cccagcgggc ggcgaggttg atccattcca tgacgagcgg gtttgtacgc
    12421 atcttttttt tcaactcccg cggtaggtgg aatagccgaa cgggctgagc aggagcggca
    12481 cgtggtagtg ctcgcccccg tcgcgcacgg cgaagacgat agccgccgcc gggtaaaaac
    12541 tctcgacgcc ctgcgccgcg aagtatgcgc ccgtgtcgaa cgtcaggcga tagacgccgg
    12601 cgcgcggctc ggcgtcgggc gcaagcagcg tccgcagccg cccgtccgag tcggtctcgc
    12661 cccgcccgag catccgccac gcgccaccct ccgcctgcac ttcgagaagc accgcgactc
    12721 cggccgccgg tctcccccgc gacgtgtcga gtacgtgtgt cgtgatcgcg ctcatatcgt
    12781 tgggaagtga taagtgatga gtgataagtg ataagacaaa
//
LOCUS       DASVES010000249         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_217839, whole genome shotgun sequence.
ACCESSION   DASVES010000249 DASVES010000000
VERSION     DASVES010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217839"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..745
                     /locus_tag="VF240_10200"
     CDS             248..745
                     /locus_tag="VF240_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7175618.1"
                     /translation="MKISAQEEYGLRCLLQLASLGEGEFLTLSQMAEREGISAANAGK
                     LLWILNKAGLVNSIRGREGGYRLARPASEVRLSEIIKVLDEDVLASHCKSYAGVLDSC
                     VHTGDCGIRPVIVGLNEVVHNALAGITLAQLVGTEAVVDATLHQINGLHSRAPSGASS
                     VGLVK"
     gene            805..>2195
                     /gene="sufB"
                     /locus_tag="VF240_10205"
     CDS             805..>2195
                     /gene="sufB"
                     /locus_tag="VF240_10205"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX7175619.1"
                     /translation="MSNIELLANREYKYGFVTDIESDVIPKGLSEDTIRTIVEKKNEP
                     EWMLEFRLKAFRHWLTMEEPNHWPNITYPVIDYQDVIYYSAPKQKAKKASLDEVDPEL
                     LKTFEKLGIPLSEQKMLSGVAVDAVFDSVSVATTYKEKLRKHGVIFCSFTEAIRDHPE
                     LIKKYLGSVVPVTDNYFAALNSAVFSDGSFCFIPKGVRCPMELSTYFRINNSESGQFE
                     RTLIIAEEGSHVSYLEGCTAPQFDKNQLHAAVVELVALDDAEIKYSTVQNWYAGDEQG
                     VGGIYNFVTKRGKCQGRNSKISWTQVETGSAITWKYPSCILQGENSVGEFYSVALTNH
                     AQQADTGTKMIHIGRNTRSTIISKGISAGRSKNSYRGLVKVLAGAEGARNYTQCDSML
                     IGSKSSANTFPYIEVMNNTARVEHEATTSRISEEQLFYLQQRGISQEDAVSLIINGFC
                     KAVFRELPMEFAV"
BASE COUNT          475 a          661 c          657 g          402 t
ORIGIN      
        1 acggagtttg ccggcgggcg tcacgccggt cgcgtcgaaa aattgaagga actagaccgc
       61 cagctcactc cacaaacctc cgactcgccc gtctgtaact gaccctcgac aagagttttg
      121 taagccgaat ccacctcgat aagtccatcg aaatttcgcc cgcactcaat cttgacaaaa
      181 aagtaaggta tgggtaaaat cctattgcgt caaagagata gcaagtttat cgggtggtgt
      241 ttgggccatg aaaatttcgg cacaggaaga gtacggtttg cgctgcctgc ttcagctcgc
      301 ctcgctgggc gagggcgagt ttttgacact gtcgcagatg gccgagcgcg agggcatctc
      361 ggcggcgaac gccggcaagc tgctgtggat tctgaacaag gccggcctcg tcaactccat
      421 ccgcggccgc gagggcggct accggctggc gcgccccgcc tcggaggtgc gtctgagcga
      481 gatcatcaaa gtgctcgacg aggacgtgct cgccagccac tgcaagagct acgcgggcgt
      541 cctcgactcc tgcgtgcata cgggcgactg cggcatccgc cccgtcatcg tcggattgaa
      601 cgaggtcgtc cacaacgcgc ttgccggcat tacgctggcg cagctcgtgg gcacggaggc
      661 cgtggtggac gcgacgctgc accagattaa cggacttcat tcgcgagcgc cctccggcgc
      721 gtcaagcgtc gggttggtca agtaaatttt caggtaatga ttccgaagcg gcgcccgcgt
      781 tggcgtgggg caggttaata aaagatgagt aacattgagc tactggcgaa cagggaatac
      841 aagtacggct tcgtcaccga catcgagtcg gacgtgatcc cgaagggctt gagcgaggac
      901 acgatccgca ccatcgtcga gaagaagaac gagccggagt ggatgctgga gtttcgcctg
      961 aaggccttcc gccactggct gacgatggag gagccgaatc actggcccaa catcacctac
     1021 ccggtgatcg actatcagga cgtgatctac tacagcgcgc cgaagcagaa ggccaagaag
     1081 gccagcctcg acgaagttga ccccgaactt ctcaagacct tcgagaaact cggcatcccg
     1141 ctctccgagc agaagatgct ctcgggcgtc gccgtcgatg ccgtcttcga ctccgtgtcg
     1201 gtggcgacca cttacaagga gaagctcagg aagcacggcg tcatcttctg ctccttcacc
     1261 gaggcgattc gcgaccaccc tgagctgatc aagaagtatc tcggctcggt cgtccccgtc
     1321 accgacaact atttcgcggc gctcaacagc gcggtcttct ccgacggctc attctgcttc
     1381 atcccgaagg gcgtgcgctg cccgatggag ctatcgacct acttccgcat caacaactcc
     1441 gagtcgggcc agttcgagcg cacgctgatc atcgccgagg aaggctcaca cgtctcctat
     1501 ctcgaaggct gcacggcgcc gcagttcgac aagaaccaac tgcacgcggc ggtggtcgaa
     1561 ctcgtcgcct tagacgacgc cgagatcaag tattcgaccg tgcagaactg gtacgcgggc
     1621 gacgagcagg gcgtcggcgg catttataac ttcgtcacca aacgcggcaa gtgtcagggg
     1681 cgcaactcga agatttcgtg gacgcaggtg gagaccggct cggctatcac gtggaaatac
     1741 ccgtcgtgca tccttcaggg cgaaaactcc gtgggcgagt tctattcggt ggcgctgacg
     1801 aaccacgcgc agcaggccga cacgggcacg aagatgattc acatcggcag gaacacgcgc
     1861 tcgaccatca tctcgaaggg catctcggcg ggccgctcga agaacagcta ccgcggactc
     1921 gtgaaggttt tggccggcgc ggagggcgcg cgcaattaca cgcagtgcga ctcgatgctc
     1981 atcggctcga aatcgagcgc caacactttc ccctacatcg aggtgatgaa taacacggcg
     2041 cgcgtcgagc acgaggcgac cacctcgcgc atcagtgagg agcagctttt ttacttgcaa
     2101 cagcgcggca tctcgcagga ggacgccgtc tcgctcatca tcaacggctt ctgcaaggcg
     2161 gtcttccgcg agctgccgat ggagttcgcc gtcga
//
LOCUS       DASVES010000250         4920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_786410, whole genome shotgun sequence.
ACCESSION   DASVES010000250 DASVES010000000
VERSION     DASVES010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786410"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VF240_10210"
     CDS             <1..793
                     /locus_tag="VF240_10210"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7175620.1"
                     /translation="PETHTPRSVRRARRRLPLMTRVATVIITPTEAVRREVCERLSVP
                     AAKVFAVPEAARAVFRPVPRSELAGVLARHGVSGDFVLAVGTVEPRKNLLALLRVFES
                     LAAEGRARHLRLVVAGSREGWLNRDFYTAVEESPVKERVVFTGYVSDDVLSALYSSCR
                     AFVYPSLYEGFGLPVLEAMACGAPVVCGDTPALAETAGGAALLVAPRDTGRLARALLE
                     IIDDEGAARRLSEAGRRRAAEFSWAHTARMTLEVYAEAMRRARVS"
     gene            complement(1043..2824)
                     /locus_tag="VF240_10215"
     CDS             complement(1043..2824)
                     /locus_tag="VF240_10215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7175621.1"
                     /translation="MGRGQEGFRATERLARLGAEARRLVVARRAELFVALLLAVMAFN
                     LLSAIGRKSITNDEVVHIPAGYYHLVSGDFRLNNEHPPLIKMWAALPLLLVQPEEPPP
                     PRTSDEDFMERTWGFHSRFWQANRARFEAVSYWPRVMMAPLALALGLVIFVSTRRLFG
                     ARAGVLAAALYTVEPTFLAHARVVHTDVAAALVYLLFFYTLYRYAETPTLRRSLLLGL
                     ACALALLTKFSMLVLLPVLAAGALARLLAVWRRGSERRRTLVHAGAVALVVLLSVNAA
                     YYFRRPALEEPDVRWVATKSAPVFKEVTGALRALSTVVPTYFLFGFYNVAIHNQYGHS
                     TSLLGRASDLGWWYYFPVAFALKTTLPFLLLSLAALLWALWRGLVRRDRRFLLLLLPL
                     AVYAAISLTSHINIGVRHFLPAYPFLFIASGALLERLLQTAGRRRVPALAMVVVVLGW
                     AGFEAARAYPHYTSYMNQLAAARPHWWYLSDSNVEWGDDVAALADYLKARGETQVRAA
                     ISGGWATLNLYGVEHVSLIDAPGHVLPETRYTAIGASYLNGSVVPGGEPGTGRESDEA
                     RRDYFAPYRERRPEAVFGNSIYLYRER"
     gene            2824..>4920
                     /locus_tag="VF240_10220"
     CDS             2824..>4920
                     /locus_tag="VF240_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175622.1"
                     /translation="MLRPYRLRPKITPERLAALAVALAPLVYFFPALAGAVVLAPDDG
                     LLFNVPLRAAAARTLLAGHLPLWNPYVFSGIPLHAAAQAGLLFPLNWLYLLFEPHWAA
                     NLSMLLTYALAGVGAFLYARRAGASLAGACATALTWQFCGFMVGQIGHTNIVQTGALL
                     PWLLWAVDGCGLGNLRPWRGLALSATVALQAFVGHSQTFAYSLLLASVYAIFNAFVSE
                     GRARRRYLWSLLFIAVGVALAAAQLLPTRELLTHSLRSEATYEFFTSFSMPSQFLWSF
                     VAPYVTGGGDGRLFRAPYTGEAFYLEYVGYAGLLALALAGLALALKRDSRTLFWAVTA
                     VVCLALASGKFLPLGLYKLIYHVPVLNLFRVPARHLLEVDFALAVLAGRGLTALESLR
                     GRKLARLSVIITSACAVVLAWVAVTAGRPNDFRLGRQAPVTLLRAPELFMPLVVAALC
                     AWALWVYARGRTRRRATLLLFVILVFDLALWGQFTGWRRGPTGEGEPWSAPEAVRVMR
                     EHADANGVSASGPYRMLTVPQVFAPDASSPPSDAASGMALMELQPNTYMMHGVENAAG
                     YDGFGLARYSRLAGDMKLWGELPDPARSLKGEGREFDLLNVRYLVARAPHQADADTVA
                     NEVETTAANAPALPAPERFGGDAFAAEDLGAAPLDRGERLLFTAPRVAAERVALVTSL
                     SWSADIADGTEVGRVRL"
BASE COUNT          715 a         1647 c         1767 g          791 t
ORIGIN      
        1 ccccgagacg cacacgcccc gcagcgtccg gcgcgcacgc cgccgcctgc cgctcatgac
       61 gcgcgtcgcc accgttatca tcacgccgac cgaggcggtg cggcgcgagg tgtgcgagcg
      121 cctgagcgtt ccggcagcga aggtcttcgc cgtgcccgag gccgcgcgcg ctgtcttccg
      181 tcctgtgcct cggtcggagt tggccggcgt gctcgcgcgc cacggagtca gtggcgactt
      241 cgtgctggcg gtcggcaccg tcgagccgcg caagaacctt ctcgcgctcc tgcgcgtctt
      301 cgagtcgctg gcggcggaag ggcgtgcgcg ccacctgcgt cttgtggtcg ccggcagccg
      361 cgagggctgg ctcaaccgcg acttctacac ggcggtcgag gagtcgcccg tcaaagagcg
      421 cgtcgtcttc acgggctacg tctcggacga cgtgctgagc gcgctctact cgtcgtgccg
      481 cgccttcgtc tacccgtcgc tctacgaggg ctttgggctg cccgtgctgg aggcgatggc
      541 ctgcggcgcg cccgtcgtct gcggcgacac gcccgcgctc gcggagacgg ccggcggcgc
      601 ggcgctgctc gtcgcgccga gagacacggg ccgactcgcg cgcgcactcc tcgaaataat
      661 cgacgacgag ggcgcggccc ggcgactctc ggaggcgggc cgccgacgcg ccgcggagtt
      721 ctcatgggcg cacacggcgc gcatgacgct ggaggtctac gcggaggcga tgcggcgggc
      781 gcgtgtgagt tgagggacac aaccagccgc gaaagcggcg gaagaagata gccacgggcg
      841 ttagcccgtg gaacagcgtt aagaaaaatc cgaaagcccc agcaggggcg aaagagtctt
      901 cacgttgggc gcggcgcccg tcgagattct ttcgcccctg ccggggcttc acgttatttc
      961 gcgtcctatt cccacggctc acgccgtggg ctacattctt ccgccgctcc gcggctggtt
     1021 gaatcgtttc tagggagacg gtctaacgct cgcggtagag atagatggaa tttccgaaga
     1081 cggcctcggg ccggcgttcg cgataggggg cgaagtagtc gcggcgggcc tcgtccgatt
     1141 cgcggccggt gccgggttcg ccgccgggga cgaccgagcc gttgaggtag ctcgcgccga
     1201 tggccgtgta gcgtgtctcc ggcaacacgt gccccggcgc gtcgatcagg ctgacgtgct
     1261 cgacgccgta gaggttcagc gtcgcccagc cgccggagat ggccgcgcgc acctgcgtct
     1321 cgccgcgcgc cttgaggtaa tcggcgagcg ccgccacgtc gtcgccccac tcgacgttcg
     1381 agtcagacag ataccaccag tgcggtcgcg cggcggcgag ctggttcatg taggacgtgt
     1441 aatgcgggta agcgcgcgcc gcctcgaagc cggcccagcc gagcacgacg acgaccattg
     1501 cgagcgccgg cacgcgccgc cgcccggccg tctggagcag gcgttcgagc agcgcgccgg
     1561 acgcgataaa gaggaagggg taggcgggca ggaagtggcg gacgccgatg ttgatgtggc
     1621 tcgtgagcga gatggccgcg tagacggcga gggggaggag caggaggagg aagcgccggt
     1681 cgcggcggac gagtccgcgc cagagcgccc acaggagcgc ggccagagag agcagtagaa
     1741 agggcagcgt cgtcttgagc gcgaaggcga cggggaagta gtaccaccag ccgaggtcgc
     1801 tcgcgcggcc gagcagggaa gtgctgtggc cgtattggtt gtggatggcg acgttgtaga
     1861 agccgaagag aaagtaggtc ggcacgaccg tggagagggc gcgcaaagct cccgtcacct
     1921 ctttgaagac gggcgcggac ttcgtggcca cccagcgcac gtccggctct tcgagcgcgg
     1981 gccggcgaaa ataatatgcg gcgttgacgg agagcaggac gacgagcgcg accgccccgg
     2041 cgtggacgag cgtgcgtcgt cgctcgctcc cgcgacgcca cacggcgagc agacgcgcga
     2101 gcgcgccggc cgcgaggacg ggcaggagca cgagcatgga gaacttcgtg agcagcgcga
     2161 gcgcgcacgc gaggccgagc aggagagagc gccgcagcgt tggcgtctcg gcgtagcggt
     2221 agagcgtgta gaagaagagc aggtagacga gcgcggccgc cacgtccgta tgaacgacgc
     2281 gggcgtgcgc gaggaaggtc ggctccacgg tgtagagggc cgcggccagc actccggcgc
     2341 gcgcgccgaa gaggcggcgg gtggagacga agatgacgag gccgagcgcg agcgcgagcg
     2401 gcgccatcat gacgcgcggc cagtaggaca ccgcctcgaa gcgcgcgcgg ttggcctgcc
     2461 agaagcgcga gtggaagccc catgtgcgct ccatgaagtc ttcgtcagag gtgcgcggcg
     2521 ggggcggctc ctcgggctgg acgaggagca gagggagcgc ggcccacatc ttgatgagcg
     2581 gagggtgctc gttgttgagt cggaaatcgc cggagacgag atggtagtag ccggccggga
     2641 tgtgcacgac ctcgtcgttg gtgatactct tccgcccgat ggccgagagc aggttgaagg
     2701 ccatcacggc gagcaggagg gcgacgaaca gctccgcgcg gcgggcgacg acgagacgcc
     2761 gcgcctccgc gccgagacgt gccagccgct ccgtcgcacg gaatccttct tgtccccgtc
     2821 ccaatgcttc gtccataccg gctgcgaccg aagataacgc cagaacgcct cgcggcgcta
     2881 gccgtcgccc tcgcgccgct cgtctacttt tttccggcgc tcgcgggcgc ggtggtgctt
     2941 gcgcccgacg acggccttct cttcaacgtc ccactgcgcg ccgcggccgc ccgcacgctt
     3001 ctcgccggcc acctcccgct ctggaaccca tacgtcttct ccggcatccc gctccacgcc
     3061 gccgcgcagg ccggtctgct cttcccgctc aactggctct acctcctctt cgagccgcac
     3121 tgggccgcga acctgtcgat gcttctgacc tacgcgcttg cgggcgtggg agcgttcctc
     3181 tacgcgcgac gcgcgggggc gagccttgcg ggcgcgtgcg cgaccgcgct cacgtggcag
     3241 ttctgcggct tcatggtcgg gcagatcggg cacaccaaca tcgtccagac gggcgcgctc
     3301 ctgccgtggc tgctgtgggc cgtggacgga tgcggactcg ggaacctgcg cccgtggcgc
     3361 ggcctcgcct tgagcgccac ggtcgctctt caggctttcg tcggccactc gcagacgttt
     3421 gcttactcgc tgctgctcgc gtccgtctat gcgatcttta acgccttcgt ttccgaagga
     3481 cgagcgcgtc gccgttacct ctggtcactc ctcttcatcg cggtcggcgt cgcgctcgcc
     3541 gccgcgcagc ttttgccgac gcgcgagttg ctcacacaca gcttgcgctc ggaggcgacc
     3601 tacgagttct ttacctcgtt ctcgatgccg tcacaatttc tgtggtcgtt cgttgcgcct
     3661 tacgtcacag gcggcggcga cggccgactc ttccgcgcgc cttacacggg cgaagccttc
     3721 tatctcgaat acgtcggtta cgcgggtctg ctcgcgctag ccctcgcggg tctcgccctc
     3781 gcgctcaagc gagattcgcg cacgctcttc tgggccgtca cggccgtcgt ctgcctcgcg
     3841 ctcgcgtcgg gcaagtttct cccccttggg ctttacaagc tgatctatca cgtccccgtc
     3901 ctcaacctct tccgcgtgcc cgcgcgtcac ctgttggaag tggacttcgc gctggcggtg
     3961 ctggccgggc gaggactgac ggcgcttgag agtttgcgcg ggcgaaagct tgcgcgcctg
     4021 tccgtcatca ttaccagcgc gtgcgcggtt gtgctcgcgt gggtggcggt gacggccggg
     4081 aggccgaacg acttccgcct cgggcgacaa gcgccggtca cactcctgcg cgcgcccgag
     4141 ttgttcatgc cgctcgtcgt cgccgcgctg tgcgcgtggg cgctgtgggt ttacgcgcgc
     4201 gggcggacgc gtcggcgggc cacgttgctt ctgttcgtca tcctcgtctt cgacctcgcg
     4261 ttgtgggggc agtttacggg ctggcggcgc gggccgacag gcgagggcga gccgtggagc
     4321 gcgcccgagg ccgtgcgcgt gatgcgcgag cacgccgacg cgaacggtgt gtcggcgtcg
     4381 gggccgtaca ggatgctcac tgtcccgcag gtcttcgcgc ccgacgcctc gtcgccccct
     4441 tcggacgcgg cgtcaggcat ggcgctcatg gagctacagc cgaacacgta catgatgcac
     4501 ggggtggaga acgcggccgg ctacgatggc ttcggcctcg cccgctacag ccgactcgcc
     4561 ggcgacatga agctctgggg cgagctgccc gaccccgcgc gcagcctcaa gggcgagggc
     4621 cgggagttcg atctgctgaa tgttcgctac ctcgtcgcgc gcgccccgca tcaggccgac
     4681 gcggatacag tggcgaatga agtggagacg acggcggcga acgccccggc actccccgcg
     4741 ccggagcgtt tcggcggcga cgcctttgcc gccgaagacc tcggagccgc accgctcgac
     4801 cgcggcgagc gtctcctgtt cacggccccg cgcgtcgcgg ccgagcgcgt cgcgctcgtg
     4861 acgagtcttt cgtggtcggc cgacatcgcg gacggcacgg aggtcgggcg cgtgcgtctg
//
LOCUS       DASVES010000251         9124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_590413, whole genome shotgun sequence.
ACCESSION   DASVES010000251 DASVES010000000
VERSION     DASVES010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590413"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF240_10225"
     CDS             <1..225
                     /locus_tag="VF240_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7175623.1"
                     /translation="AARGFSAVTNLSDREAEILRLIALGHINKEVAARLSLSVKTVEA
                     HKANAMRKLGMQSRVDIVRYALLQGWLQES"
     gene            637..1035
                     /locus_tag="VF240_10230"
     CDS             637..1035
                     /locus_tag="VF240_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175624.1"
                     /translation="MPTQTPNACVMVAEDHEDTRFLLRTMLEMRGGISVVEAANGEMA
                     LALAMNVHIDLVLMDIDLPLLDGYTVTRRIRELASTHEIPIVIISGHAQPAAEALAFA
                     AGCTEYLVKPFALVKLGSVLERHLSQSKAN"
     gene            1071..1421
                     /locus_tag="VF240_10235"
     CDS             1071..1421
                     /locus_tag="VF240_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175625.1"
                     /translation="MSSVIFAPQKLFGMLELDAAGTVLYSRLEGEDKSDVPAASVAGH
                     NFYSEVAPFLNVEEFQQRLDIFNQSSDQARSFVFNCDYEDGAVPVRVLLARISGRVND
                     ERIKSLLVHIKKAD"
     gene            1668..2651
                     /locus_tag="VF240_10240"
     CDS             1668..2651
                     /locus_tag="VF240_10240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7175626.1"
                     /translation="MKPESMLEKEREPEQAAEERASVILLADDEPVNLSLIKRRLEWE
                     NYEIHTAQDGREAVARARELLPDLIILDVMMPIMDGLQACRFLKDDAATRDIPVIFLS
                     ALDDMETKVSGLSLGANDYISKPFRAEELVARVGVAIRLKRERDHLRESAEDASRRAE
                     AASEMSMTDPLTGLLNRYGLRRSLQHEYAEARRYSRPLSCLMIDLDHFKRVNDTHGHA
                     AGDAALMQVARVLTEAVRASDVVCRYGGEEFLVLAPETSVEGARSLGEKIRAAATARL
                     FGDGAAAFPLTFSVGVAQLGPGESAHDMVVRADHALYHAKEAGRNRVEAAA"
     gene            complement(2730..3869)
                     /gene="pabB"
                     /locus_tag="VF240_10245"
     CDS             complement(2730..3869)
                     /gene="pabB"
                     /locus_tag="VF240_10245"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX7175627.1"
                     /translation="MNYPTLIFDFADERGQPRRLCFSRPVEVVEARTPREVRPALRRV
                     EEAVGAGYYVGGFIAYEAAPAFDSSLVVRDSAASASALPLLWFALFNAPDAHGPVEDE
                     QARFSVSNWSPSIDRHAYERSVAAVREAVARGDTYQTNYTFRLRAHFAGDALAFYRYL
                     CARQRADFCAFLDTGRHKILSASPELFFRQRGRRILTRPMKGTARRGRNAEEDAAQSR
                     WLEGSEKNRAENVMIVDLMRNDLGRVAETGSVRASELCRVETYPTVFQMTSAVEATLR
                     DGATLEDVLAALFPSGSVTGAPKVSTMRLVAALEDSPRGVYCGSVGLIEPGGAAVFNV
                     AIRTVVIDSETGAAEYGTGGGITWDSAAAEEYDEALAKAALLSDK"
     gene            complement(4068..5261)
                     /locus_tag="VF240_10250"
     CDS             complement(4068..5261)
                     /locus_tag="VF240_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX7175628.1"
                     /translation="MKKVVLRVVVLLALLAATCLLAAAPCAQGQTRPRARDLGVPFDG
                     TPGPLNAITDVRGVEVGFVTLVEGEGKLVMGRGPVRTGVTAVFPQGKSFKGRVFAAWD
                     TLNGNGEMTGTTWVEESGGLGTPILITNTHSVGVVRDAVIEWNVRRGTGEGYSGDFSL
                     PVVAETWDGFLNDINGFHVRREHVFQALDGAKPGPVAEGNVGGGTGMVAHGFKGGTGT
                     SSRVLNADNGGFVVGALVQANYGRRDLLRIAGVPVGVELTDLMPERGKTGADGEGSII
                     VVVGTDAPLLPHQLKRLARRVALAVGVMGGRGEDSSGDIFIAFSTANPAEVSKTAGTA
                     QLAMLPNERINPVFRAAVEATEEAIVNAMVAAETMRGINGNTVHAIPHERLREVLRKY
                     NRLAK"
     gene            complement(5272..5721)
                     /locus_tag="VF240_10255"
     CDS             complement(5272..5721)
                     /locus_tag="VF240_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175629.1"
                     /translation="MSSEDVRPAPIKSTPMIQRVGIYTAVLLAGLLLGFVPMWVKARQ
                     CAGSLAESGHHLVLARMQSNLASAAIDARRGDYEPARQAASEFYTSLRAEADRGEASV
                     LTQPQRAGVQPLFGQRDEIITLLARSDPASADRLSDLYASYRKIVGG"
     gene            complement(5764..6612)
                     /locus_tag="VF240_10260"
     CDS             complement(5764..6612)
                     /locus_tag="VF240_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175630.1"
                     /translation="MNTMSLRGKGAVASMFFLAAAMLGCNSSGGVNSNAPAPPASANT
                     ATANTTTPPAKPSADGTIASGTGVEKEKPAAGKGNVQGKALYNEQPAVGVEVKLCEKF
                     NQYLGGCSGETFTSRTDSAGEYLIKDVPPRVYEGLMVKVFDTGYYVFATSGIVQTAKY
                     NIEADKTFFAPVSHLFKSDLKLASPKAGSKIGGSNIEVKWDAYPDAAYYKFSIFADTA
                     SGAKPEYDFINKRVDGLSYVLDKPLSPGSYTCKVEAYNGNDIRLAQSSSDIKFTVTGE
                     APKSSP"
     gene            complement(6683..7384)
                     /locus_tag="VF240_10265"
     CDS             complement(6683..7384)
                     /locus_tag="VF240_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175631.1"
                     /translation="MSTSTVPIDKLKHQETCDELTRFVEARVLKNIVGKQPDERWTRD
                     DMDLRLQLKTHRDELFRQEFLAHPRFIYALAVEESLGIPSFAFLRVIDSVAVLQETPE
                     RLYLVLPSCHKGCEARPAAPPEGVCAGSCHVCGITFPTHTDNRSTCEQWEAVNSTAPT
                     RKEIEDLLKKKAALDHPFKKELTSRPMEIYTAFATKVCGGMEPSYMAAVKEIQVLEEG
                     ERDVYFLLQAQTEDA"
     gene            complement(7745..8275)
                     /locus_tag="VF240_10270"
     CDS             complement(7745..8275)
                     /locus_tag="VF240_10270"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX7175632.1"
                     /translation="MRKVEVVPHNSQWREAFEAEAKYVAAALGECVVAVHHIGSTAIP
                     DIYAKPVIDLLVEVRDIAEVDGQSSAMESLGYEVMGEYGIPGRRYFRKDNRDGIRTHN
                     LHAFRAGSAEVERHLAFRDYMIAHPGDAQRYSELKRKLAEDHPRSFDGYMDGKDGFIK
                     EIDRRAARWRPSQTAQ"
     gene            complement(8477..8920)
                     /locus_tag="VF240_10275"
     CDS             complement(8477..8920)
                     /locus_tag="VF240_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX7175633.1"
                     /translation="MKKAAVLFVGLMCVGLFGFPTKAGRFGDGGHLPSDPPRTVVARE
                     WKGRVAPARAEEYYRYLRGGVTKLRSIAGSLGVEVMRREEAGAVEYTVISYWESREAI
                     KAYAGQDIEKPHHLPKDRELLLELPTRVLHYDVAYTDLTQVRVGQ"
BASE COUNT         1709 a         2962 c         2688 g         1765 t
ORIGIN      
        1 gccgctcgcg gcttttctgc cgtcacgaat ctgagtgacc gcgaggcgga gattctgcgc
       61 ctcatcgcgc tgggccacat caacaaagag gtggccgcgc gcctctccct gagcgtcaag
      121 accgtcgagg ctcacaaggc caacgcgatg cgtaaactgg gcatgcaaag ccgcgtggac
      181 atcgtgcgct acgcgctcct gcaaggctgg ctgcaagaat cctgacgcct cccgagccgc
      241 cccggcgcat ccgctcgccg tcagggtaaa acccttgccc ctttcactca ccttcccgat
      301 atacgaaact cgccgtgtaa ggtaaaaact aaacgcaatc ggcccgcaag gcgtaagcgc
      361 cacgcttccc gcattaccgg aggcatgcgt ttgacggccg actgtctcgt tcgctgtgtg
      421 cggggaggaa acggccagcc ggacacagtg ggacgccggc ggaagacggg tgaagttttg
      481 tcacgagact tcacccttcc acctccgctc cagatagttt cggaaatagc agcgaagtgc
      541 ggttcgctca cacaatttcc gcactttcct accatgtcaa caggaacgag ttacagcccc
      601 ggacccggcc caaccgccaa tattcaacgt gcgcctatgc cgacgcaaac ccccaatgcg
      661 tgcgtgatgg tagcggaaga tcacgaggac acgcgcttcc tgctgaggac gatgcttgag
      721 atgcgcggcg gcatcagcgt ggtcgaagca gcaaacggcg agatggccct tgccttagca
      781 atgaacgtgc atatcgacct cgttctgatg gacatcgacc tcccgctgct cgacggatat
      841 acggtgaccc gacgcattcg tgaactcgct tccacgcatg aaatcccgat tgtcatcatc
      901 tccggccacg cccagcccgc ggccgaagca ctggcgtttg ccgccggctg caccgaatac
      961 ctcgtcaagc cgtttgcctt agtcaaactt ggcagcgtgc tggaacgaca cctttcacag
     1021 agcaaagcga attagatagg ccgcccccag agcggtagag gatttccgta atgagcagtg
     1081 tgatttttgc accacaaaaa ctcttcggga tgctcgaact cgacgcggcg ggcacggtgc
     1141 tctattccag attggaaggc gaagacaagt cagacgttcc ggcggcgagt gtggcgggac
     1201 ataatttcta ctccgaagtc gctcccttcc tgaacgtcga agagtttcag caacgtctcg
     1261 acattttcaa ccagagcagc gatcaggcgc ggagctttgt cttcaactgc gattacgaag
     1321 acggcgcagt ccccgtcaga gtccttctgg cgcgcatcag cggtcgtgtc aacgatgagc
     1381 gcatcaaatc attgctcgtg catatcaaga aagccgatta accttccatc caggtctgct
     1441 tagacttcgc acgcgccgac gccgtcggcg cgtctttcgt taagcgcatc atcaaccccg
     1501 cttgtcgtcg cccgcccgcg tgttttaaaa ttgagactgc acccggccgg cggttttcgg
     1561 ttctcccgcc agcccttgcc ccctcctcat aaaccgcgcg ccgcgtctcg accacgacca
     1621 atgagagtcg gggtgcgtct cgcggcccgt tcaggggcga ttcgtttatg aaaccagagt
     1681 cgatgttgga gaaagagcgc gagccggagc aggcggcgga agagcgggcg tccgtcatcc
     1741 ttctggcgga cgacgagccg gtcaacctct cgctcatcaa gcggcggctg gagtgggaga
     1801 actacgagat tcacacggcg caggacgggc gcgaggccgt ggcgcgcgcc cgcgaactcc
     1861 tccccgacct gatcatcctc gacgtgatga tgcccatcat ggacggcctt caggcgtgcc
     1921 gcttcctcaa ggacgacgcc gccacgcgcg acatccccgt catcttcctc tccgcgctcg
     1981 acgacatgga gacgaaagtc agcggcctct ctttgggcgc caacgactac atctcgaagc
     2041 ctttccgcgc cgaggagctg gtggcgcgcg tcggcgtcgc catccgcttg aagcgcgagc
     2101 gcgaccacct gcgcgagagc gccgaagacg cgagccgccg ggccgaggcc gccagcgaga
     2161 tgtcgatgac cgacccgctg actgggctgc tgaaccgcta cgggctgcgg cgctcgctcc
     2221 agcacgagta cgccgaggcg cgccgctact cgcgcccgct gtcctgcctg atgatcgacc
     2281 tcgaccactt caagcgcgtc aacgacacgc acggccacgc ggccggcgac gccgcgctca
     2341 tgcaggtggc gcgggtgctg accgaggccg tgcgcgcctc cgacgtggtc tgccgctacg
     2401 gcggcgagga gtttctcgtg ctcgcgccgg agacgagcgt cgagggcgcg cgctccctcg
     2461 gcgagaagat tcgcgccgcg gccaccgccc gtctcttcgg cgacggcgcc gccgccttcc
     2521 cgctcacctt cagcgtcggc gtcgcccaac tcggccccgg cgagtcggcc cacgacatgg
     2581 tcgtccgcgc cgaccacgcc ctctaccacg ccaaggaggc gggccgcaac cgcgtcgaag
     2641 cagcagcgtg aaaaagtgat aagtgatgag tgataagtga taagaaagaa gagggtcggc
     2701 ttctactctt tcttatcact tatcactcat cacttatcac ttaacaaagc tgccttcgcg
     2761 agcgcctcgt cgtattcctc ggcggcggcg gagtcccagg tgatgccgcc gcccgtgccg
     2821 tactcggccg cgcccgtctc cgagtcgatg acgaccgtgc ggatggcgac gttgaagacc
     2881 gccgcgccgc ccggctcgat gagtccgacg ctcccgcaat agacgcccct cggagaatcc
     2941 tccagcgccg ccaccagacg catcgtgctc accttcggcg cgcccgtcac cgaccccgaa
     3001 gggaagagcg ccgccagcac gtcttcgagc gtcgcgccgt cgcgcaaggt agcctcgacc
     3061 gccgaggtca tctggaagac ggtcgggtac gtctcgacgc gacagagttc gcttgcgcgc
     3121 accgacccgg tctccgcgac ccgtcccaga tcgttacgca tcagatcgac gatcatcacg
     3181 ttctccgcgc gattcttctc cgagccttcg agccagcgcg actgcgccgc gtcctcctcg
     3241 gcgttgcgcc cgcgccgcgc cgtgcccttc atcggccggg tgaggatgcg ccggccccgt
     3301 tggcggaaga agagttcggg ggaggcggag aggattttgt ggcggccggt gtcgaggaag
     3361 gcgcagaagt cggcgcgctg ccgggcgcag aggtatcggt agaaggcgag ggcgtcgccg
     3421 gcgaagtgtg cgcgcagtcg gaaggtgtag ttcgtctggt aggtgtcgcc gcgcgcgacg
     3481 gcctcacgca ccgccgcgac gctccgctcg taagcgtggc ggtcgatgga cggcgaccag
     3541 tttgagacgc tgaaacgcgc ttgctcgtcc tccaccgggc cgtgcgcgtc gggcgcgttg
     3601 aagagtgcga accagaggag cggcagcgcc gacgccgacg ccgcgctgtc gcgcacgacg
     3661 agcgatgagt cgaacgccgg ggcggcctcg taggcgatga agcctcccac gtaataccca
     3721 gcgccgacgg cctcctcgac gcgccgcagc gcgggcctga cctcgcgggg cgtgcgtgct
     3781 tcgacgacct cgacggggcg cgagaagcag agtcggcggg gctggccgcg ctcgtcggcg
     3841 aagtcgaaga tcagggtggg atagttcacc gaactcttcg tgtttggtac gacgtattaa
     3901 ggaacgtcct accttgactc ggatcgagtg tgtaacgcaa ggctattcac cgcagagacg
     3961 cagaggacgc agaggaagcg cggaggcttg aatcaactct ctgcgtgaac tccgcgtcct
     4021 ctgcggtgag tccaactctc cacacactca agggagaggc gcagtctcta cttcgccagc
     4081 cggttgtact ttctcaaaac ttcgcgcagg cgttcgtgcg ggatggcgtg gacggtgttg
     4141 ccgttgatgc cgcgcatcgt ctcggccgcg accatggcgt tgactatcgc ctcctcggtc
     4201 gcttcgacgg ccgcgcgaaa gaccgggttg atgcgctcgt tcgggagcat cgcgagttgc
     4261 gccgtgcccg cggtcttgct cacctcggcg gggttggccg tcgagaaggc gatgaagatg
     4321 tcgcccgagg agtcctcgcc ccgcccgccc atcacgccga cggccagcgc cacgcgccgc
     4381 gcgagccgct tcaactggtg cggcagaagc ggcgcatccg tccccaccac gacgatgatc
     4441 gacccctcgc cgtccgcgcc cgtcttgccg cgctccggca tgaggtctgt caactccacc
     4501 ccgacgggca cgccggcaat ccgcagcaga tcgcgccggc cgtagttggc ctgcacgagc
     4561 gcgccgacca cgaagccgcc gttgtccgcg ttaaggacgc gcgaggaggt gcccgtcccg
     4621 cccttgaagc cgtgcgcgac catccccgtc ccgccgccga cgttgccctc ggcgaccggc
     4681 cccggcttcg cgccgtcgag cgcctgaaag acgtgctcgc ggcggacgtg gaagccgttg
     4741 atgtcgttca aaaatccatc ccacgtctcg gccaccacgg gcaggctgaa gtcgcccgag
     4801 tagccctcgc ccgtcccgcg ccgcacgttc cactcgataa cggcgtcgcg caccacgccg
     4861 acgctgtgcg tgttcgtgat gagaatcggc gtccccagtc cgcccgactc ctcgacccac
     4921 gtcgtccccg tcatctcgcc gttgccgttc aaggtgtccc acgcggcgaa gacccgtccc
     4981 ttgaaactct tcccctgcgg gaagacggcc gtgacgccgg tgcggacggg gccgcgcccc
     5041 atgacgagct tgccctcgcc ctcgacgagc gtcacaaacc cgacctccac gccgcgcacg
     5101 tccgtgatcg cgttgagcgg tcccggcgtg ccgtcgaagg ggacgccgag gtcgcgcgcg
     5161 cgcggtctgg tctgcccttg cgcgcacggc gcggcggcga gcagacacgt cgccgccaga
     5221 agcgcgagca ggactactac gcgcaacacg acttttttca ttcgactatc ctcagccgcc
     5281 cacgatcttg cgataggacg cgtacaggtc ggacaggcgg tctgcggaag ccgggtcgct
     5341 tctggcgagc agcgtgatga tctcgtcacg ctgcccaaac aacggctgca ctcccgccct
     5401 ctgcggctgc gtgaggacgg aagcttcgcc cctgtcggcc tccgcccgca gtgacgtgta
     5461 gaactcactg gcggcctgac gcgccggctc gtagtcgccc cggcgcgcgt caatcgccgc
     5521 cgacgccaga ttactctgca tccgggcaag caccagatga tgccccgact cggccagact
     5581 gccggcgcac tgacgagcct taacccacat cgggacaaag ccgagcaaga gaccggcgag
     5641 cagcacggcc gtataaatac cgacacgctg aatcatcggc gttgacttga tgggtgcggg
     5701 tcgcacgtct tcactgctca taaccactcc ttatctaact gccctacttg gttggttcgc
     5761 ccgtcacggt gaactttttg gcgcttcgcc cgtcacggtg aacttgatgt cgctcgaact
     5821 ttgagcgagc ctgatgtcgt tgccattata ggcttccacc ttgcaggtgt atgaacccgg
     5881 cgaaagcggt ttatcaagga cgtaagacag cccatcaacg cgcttgttga tgaagtcgta
     5941 ctccggcttg gcccccgacg cggtgtcggc gaagatgctg aatttgtaat aggccgcgtc
     6001 ggggtaagcg tcccacttca cctcgatgtt gctcccgccg attttcgagc cggccttcgg
     6061 actcgcaagc ttcaaatcgc tcttgaacag gtgtgagacg ggcgcgaaga acgtcttgtc
     6121 agcctcgatg ttgtacttgg ccgtctggac gatgcccgaa gtcgcgaaca cgtaatagcc
     6181 cgtgtcgaac actttcacca tcaagccttc gtaaactctg ggcggcacgt ctttaatcaa
     6241 atactcgccc gcactatccg tccgggacgt gaaagtttcg ccgctacacc cgccaaggta
     6301 ctgattgaat ttctcgcaca gtttaacttc aaccccgacg gccggctgtt cgttatacag
     6361 cgccttgccc tgcacgttac ctttgccggc ggcgggcttt tctttttcca cgcccgtgcc
     6421 cgaagcaatc gtgccgtcgg cgctcggctt ggcgggcggc gtggttgtat tcgccgtggc
     6481 tgtattcgca ctggccggcg gggccggtgc attggagttt acgcctccgg aagagttgca
     6541 gcccagcatg gccgcagcca aaaagaacat ggaggcgacg gcccctttac ctctcaacga
     6601 cattgtgttc attctcctct cttcagttta tctcttcaac gtcggctccc gaccacgtcc
     6661 cctccgcagc ccagtcgatt attcaggcat cctcggtctg agcctgaagc aggaaataaa
     6721 cgtctctttc cccttcctca agcacttgaa tttctttcac ggcagccatg tagctcggct
     6781 ccatgccgcc gcagaccttc gtcgcgaagg cggtgtaaat ttccatcggc cgggaagtta
     6841 attctttctt gaatggatga tcgagggccg ctttcttctt gagcaaatct tctatctctt
     6901 tccgtgtggg cgcagtgctg ttgacggctt cccactgctc gcaggtactg cggttgtccg
     6961 tgtgcgtggg aaaggtgatg ccgcagacat ggcatgagcc agcgcaaact ccttcgggcg
     7021 gcgcggccgg ccgcgcttca caacctttgt ggcaggaggg cagcacaagg tacaaacgct
     7081 caggcgtctc ttgcagcacg gccacggaat cgatgacgcg caggaaagcg aaacttggaa
     7141 tccccaggct ctcttcgacg gccagcgcat agataaagcg cgggtgagcc agaaactcct
     7201 gcctgaagag ttcatcgcga tgggttttaa gttggaggcg caggtccatg tcatcgcgtg
     7261 tccagcgctc gtccggttgt ttgccgacaa tgtttttaag cacgcgcgcc tctacaaatc
     7321 tggttaattc gtcgcaggtc tcctggtgtt tgagcttgtc gatgggaacg gtgctggtgg
     7381 acatctcagg gtctcctttc gttgttaacc actagttgtc taaaggaagt gaagcgccaa
     7441 ggaagctgag ttcacggctg agcagtaaaa gcgcgtgcga atctgttcgg atatcaatga
     7501 aaccgatgat ggataaccaa aaagtttggc caatctagca cagaacacat aggccaacaa
     7561 ggtttttgaa ctggcttacc gcggagcaaa ataaatgact gaagctggat caccaacaga
     7621 ccccgagggc cagtttgaaa cgaggtggat agtgtcgaat ttcactgttt gatcgtgatc
     7681 gagcggcggc gcatgatctt cttttcaaaa tcagcgatga cgcggcggct tgttaggctg
     7741 ctgcctactg agcggtctgc gatggacgcc aacgtgctgc ccgccggtct atttctttga
     7801 tgaaaccatc tttgccatcc atatacccgt cgaagctccg cgggtgatcc tccgccagct
     7861 tcctcttgag ctcgctgtat ctttgtgcat ctccggggtg agcgatcatg taatcgcgga
     7921 acgccaggtg acgctctacc tcagccgagc cggcccggaa ggcatgaagg ttatgcgttc
     7981 tgattccgtc ccggttgtct ttacggaagt accggcgacc ggggatgccg tactcaccca
     8041 tcacttcata accaagtgac tccatcgccg aactctggcc atccacttca gctatgtctc
     8101 tgacctcgac taacaaatcg atgacgggtt ttgcatatat gtcgggaatg gcggtgctgc
     8161 cgatatggtg gacggcgaca acacactcgc ccagtgcggc ggccacatac ttcgcttccg
     8221 cctcaaatgc ctctcgccac tgagagttgt gggggacaac ttctactttc ctcatcaatc
     8281 aggcgcctca cgagtagctt ctaaaccacc accgcaaacg atgatctgag cagcttaatc
     8341 cccggcatca cccgccgcgc acgaggactt tgatgagtaa gagaaaagct ggggtgcgcg
     8401 tccgctgcac gtgtggtcgt tgagcgacgc caccgaaggc atctgtcgcc cacgccttca
     8461 ctcacggtgg cgcactctac tgaccgactc ggacctgcgt taggtcagta taggcgacgt
     8521 cataatgaag cacgcgtgtc ggaagttcga gcagcaactc ccggtctttg gggaggtgat
     8581 gaggtttttc gatgtcctgc ccggcatatg ccttgatcgc ttctcgtgac tcccagtacg
     8641 aaatcacggt gtactcgacc gcacccgcct cttcgcggcg cattacttcg actccgagac
     8701 tcccggcaat cgagcggagc ttcgtcacgc ccccgcggag gtagcggtaa tactcctcag
     8761 cgcgtgccgg cgcgacccgc cctttccatt cacgagcgac aaccgtcctc ggcgggtccg
     8821 agggcaggtg tccgccgtcg ccgaaacggc cggccttagt agggaagccg aatagcccga
     8881 cgcacatcaa tccgacgaag agcacggcag ccttcttcat atgagttctc ctgtcttcac
     8941 gtaggatcac caagcagccc aacgcccgtc atcacccgcc gcgcacggcc tttgaattgc
     9001 gggtgtctcg cggattaaat caacgttggc tcgcggtcgg gtgcatgcca ttgttagatt
     9061 gcggcacaga aaatgtttcc ccgattaagc taaaccccat gtcttttcta atctcaccgt
     9121 ctct
//
LOCUS       DASVES010000252         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_233755, whole genome shotgun sequence.
ACCESSION   DASVES010000252 DASVES010000000
VERSION     DASVES010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233755"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VF240_10280"
     CDS             complement(<1..809)
                     /locus_tag="VF240_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7175634.1"
                     /translation="MAFKSLFSLFSSDLAIDLGTANTLVYAKGRGIVVSEPSIVAINK
                     VTNQVEAVGKEAKEMLGRTPGNIVAIRPMRDGVIANFEVTEKMLQHFIRKAHNGKTWV
                     SPRVVIGIPSEITQVERRAVEDSAYRAKASEVYLVEEAMAAAIGAGLPITEPYGNMIV
                     DIGGGTTDIAVISLSGIVYSRAIRVAGNEMDESITSYIKRKYNLLIGERTAEAIKIQL
                     GSAFPLDEQLSMDVRGRNLIEGIPKTITITDDEVREALADSISTIVNAVRVA"
     gene            complement(1155..>2354)
                     /locus_tag="VF240_10285"
     CDS             complement(1155..>2354)
                     /locus_tag="VF240_10285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7175635.1"
                     /translation="LERLGFHAVADEPAQLPALLRALESRGLTGELRAPEPDERAAAR
                     AAETPPASLLLPERLGVEQLRAAFETVASLHFSNDQRSAASSALVGLAALVAAERWTI
                     YLVSEASRDTVNFEPLAARGLTRGGRELPDDDWRRTLFGDALALAGAESAAAREAVER
                     AETICGAEAERFVLAVPLVDGERVMGVLESVREGAGASEFSAADESLLSAMALPLAAA
                     LSNSVRIAEAERLSQTDDLTKLHNARFLRQYLIAEVRRARRYGSMVSVMFLDLDDFKA
                     VNDRHGHLVGSHVLMEMAAVILSSVRDTDVVSRYGGDEFVVILPETGAEPSARVAERV
                     RATVSAHAFTGGRNLHLRLTASFGVASFPAHANSPQQLVAAADAAMYEAKAARKNCVR
                     FAPEGAA"
BASE COUNT          376 a          745 c          847 g          386 t
ORIGIN      
        1 gcgacgcgga cggcgttgac gattgtcgag atcgaatcgg cgagagcttc gcgcacttcg
       61 tcatcggtga tcgtgatggt cttcgggatg ccttcgatga gattgcgccc gcgcacgtcc
      121 atcgagagtt gttcgtcgag cgggaaggcc gagccgagct gaatcttgat ggcctcggcc
      181 gtgcgctcgc cgatgaggag attgtacttg cgcttgatgt aggacgtgat ggactcgtcc
      241 atctcgttac cggccacgcg gatagcgcgc gagtagacga tgcccgagag tgagataacg
      301 gcgatgtcgg tggtgccgcc gccgatgtcc acgatcatgt tgccgtaagg ctccgtgatg
      361 ggcaggcccg ccccgatggc cgcggccatc gcctcttcga cgaggtagac ttcggacgcc
      421 ttggcgcggt aggccgagtc ttcgaccgcc cggcgctcga cctgcgtgat ctccgagggg
      481 atgccgatga cgacgcgggg gctgacccac gtcttgccgt tgtgcgcctt gcggatgaag
      541 tgctggagca tcttctccgt gacctcgaag ttggcgatca cgccgtcgcg catcggccgg
      601 atggcgacga tgttgccggg cgtgcggccc agcatctcct tggcctcctt gcccacggcc
      661 tccacctgat tggtgacttt gttgatggcg acgattgatg gctcgctgac gacgatgccg
      721 cgccccttcg cgtagacgag cgtgttggcc gtgccgaggt cgatagccag gtcgctcgag
      781 aacagactga agagtgattt gaatgccatt tagtttccgc ccgtgcctgc ccttctatga
      841 tgacgtaatc tgcgaaaaac cctcgcggcg ccggggcaac cccggccgcc cggccgcgtg
      901 tgaatcaact gccggccggc ggccgcgctc gtgctcgccc tctcgctcgc gcttcgaact
      961 caaaagaata caacgaagcg acgcggcgta gccaaccggg aaagaagtcc ggagggcggc
     1021 ggggcgagcg agagactgcc gtgctgtcgt gttttgggtc caaccaggcg gggggcctta
     1081 tggtcctgga aaccttcgga ttattgctta cagcaagagg cgggaaatgt ctacagaaat
     1141 tttttcgagc cgcgtcaggc cgcgccctcg ggagcgaagc ggacgcagtt cttgcgcgcg
     1201 gccttggcct cgtacatggc ggcgtcggcg gcggcgacga gttgctgcgg gctgttggcg
     1261 tgggcaggga aggaggcgac gccgaagctg gccgtcaggc ggagatgcag gttgcgcccg
     1321 cccgtgaagg cgtgggcgga gacggtcgcg cggacgcgct cggcgacgcg ggccgacggc
     1381 tccgcgcccg tctcgggcag gatgacgacg aactcgtcgc ccccgtagcg cgacaccacg
     1441 tccgtgtcgc gcacggagga gaggatgacg gcggccatct ccatcaggac gtgcgagccg
     1501 acgaggtggc cgtggcggtc gttgacggcc ttgaaatcgt cgaggtcgag aaacatgacc
     1561 gagaccatgg agccgtagcg gcgggcgcga cgaacttcgg cgatcagata ctgccgcagg
     1621 aagcgcgcgt tgtggagctt ggtgaggtcg tcggtctgcg acaggcgctc ggcctcggcg
     1681 atacgcacgg agttcgacag cgccgccgcc agcggcaacg ccatcgccga gagcagcgac
     1741 tcatcggcgg cggagaactc actcgcgccg gcgccctcgc gcacggactc caacaccccc
     1801 atcacgcgtt cgccgtccac gagcggcacg gccagaacga atcgttcggc ctccgcgccg
     1861 cagatcgtct cggcgcgctc gaccgcctcg cgcgcggccg ccgactcggc gccggcgagc
     1921 gccagagcgt cgccgaagag tgtccgcctc cagtcgtcgt cgggcagttc gcgcccgccc
     1981 cgcgtcaggc cgcgcgcggc cagtggctcg aaattaactg tgtcgcggct cgcctccgag
     2041 acgaggtaga tcgtccagcg ttcggccgcg acgagcgcgg cgagtccgac gagcgcggac
     2101 gacgcggccg aacgctggtc atttgagaag tggagcgagg cgaccgtctc gaaggcggcg
     2161 cgcagttgct cgacgccgag gcgctcgggc agcagtaggc tcgcaggagg cgtctcggcg
     2221 gcgcgcgccg cggcacgttc gtccggctcg ggcgcgcgca gctcgcccgt caggccgcgc
     2281 gattcgagag cgcgcagcag ggccgggagt tgcgccggct cgtcggccac ggcgtggaag
     2341 ccgaggcgtt cgag
//
LOCUS       DASVES010000253        21624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_781672, whole genome shotgun sequence.
ACCESSION   DASVES010000253 DASVES010000000
VERSION     DASVES010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781672"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VF240_10290"
     CDS             complement(<1..296)
                     /locus_tag="VF240_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175636.1"
                     /translation="MEDIGIGERVEAESAAPTSLPAERVRVETGVRDRTILFVVGALL
                     VGFSFWWTQFSTQAICCGDYDGYYHFRLARMLQDGMRSGQFPPAFESLPLTTLNA"
     gene            complement(434..571)
                     /locus_tag="VF240_10295"
     CDS             complement(434..571)
                     /locus_tag="VF240_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175637.1"
                     /translation="MRPLLSRLFAALLTFASGVYASGLWYQLHECNAMLGPLCCGMAP
                     Q"
     gene            complement(622..1494)
                     /gene="ispH"
                     /locus_tag="VF240_10300"
     CDS             complement(622..1494)
                     /gene="ispH"
                     /locus_tag="VF240_10300"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7175638.1"
                     /translation="MQVLLAEEFGFCFGVERAVEMVEEALQEGATVRTLGPLIHNTQE
                     IGRLESEGVRTIDAPAEVESGVTAVIRAHGVTPEVQRELEARATKVVDATCPFVTKVQ
                     ILAQRAAAEGRDVVVVGSPDHPEMIGVKGYAPQNAHVVRDASEVALLPFLHAPLVVSQ
                     TTLKLSTFLEAAEAVRAKSDAEPQVVNTICSATRDRQDAARALAGEVEVFYIIGGKHS
                     SNSIKLLSVCKEQCPRSFLIETPEEIDPEDVRGAERVGVTAGASTPNWLIEQVVARLK
                     EIGAEHEAAVPVGR"
     gene            complement(1583..1891)
                     /locus_tag="VF240_10305"
     CDS             complement(1583..1891)
                     /locus_tag="VF240_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175639.1"
                     /translation="MNCSDNNDPRYKTGNPVCDALRFLCDASFAVLPRDAAYKLGEFE
                     KNFWGGVRWFADKNMSWVDESLAAGDRLREEWNSRRQRSASYTDPTTPPTAEGPAGGI
                     "
     gene            complement(2066..2680)
                     /locus_tag="VF240_10310"
     CDS             complement(2066..2680)
                     /locus_tag="VF240_10310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7175640.1"
                     /translation="MSREEKPPVVYVALGDSTGVGVGARNGGGYVARLFERLERARPG
                     SRLVNLCVSGATTADVLRAQVGRVRQERPTLVTLGIGINDLTRMVAPEQFARNFEEIV
                     TRLREATPAPLVVANIPDISLAPVVPVFLRAAASQRIQTFNASLAAVARAHGLRLLDA
                     YAFSHELIPTHPEFFSPDGFHPSEEGYEFWAVKMWPEIKEAIGE"
     gene            complement(2776..5646)
                     /locus_tag="VF240_10315"
     CDS             complement(2776..5646)
                     /locus_tag="VF240_10315"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7175641.1"
                     /translation="MGRQRGGGGRGPRGGLKRGGRGGRTSGGAQDERGARDGATRGDA
                     LRRDGGGRGRATTRPAASPAETAQVSHVAGRASRTRPVRQLLEGIGAPAPAPFVPDPF
                     QLEALAALEHEDVLVTAPTGSGKTWIAREEIRRLLEQGRRAWYTSPLKALTNSKFQEF
                     SAEFGVGTVGVLTGDRKENSEAPLVVGTTEVYRNQLFDALRGGRQVDVDLVILDEAHY
                     LADEERGHVWEEAIILTPPRVRMLLLSATVGRAEEFAEWIAEVRGHACRVVARPGARP
                     VPLRAAFLFPDGQIAPLFDERNHFNSEIARFMQTSRSERRPEPHTRFGARGGAPQRPR
                     MPEMPPSTLLTALSSYDLLPAIVFLPTRRRCDEAAVESAHAPRANNNTHRREARRVLL
                     DELVEQYPEVRRHRHWDMVVRGGVASHHAGHLPAWKLAIEKLMSAGLLDAIFATATVA
                     AGVDFPARTVVLANIDVRTGRGWRVLTASELQQMTGRAGRRGRDRVGFIVAAPGQHQD
                     PHKLAALLHSPPDPLESQFRATYTTLLNLLDAYGTFSQVRDIAERSFSQREAGERLTG
                     LERERAETEGRMRDRLGEAGCQLPPEVARGLERLASARSRLLEGAPHTRFEATLRWLD
                     KEVVPGRVVGVGRSGRRLVFVTERRADGLVGYREDGRRASVALERIGRVYATIFARDE
                     SARDEAFESVKRGKAEPISEPRLRDSRADADGAVDLINELIESLSAGVAEGEERTRCE
                     EILWSVVKEAEGLERAERQAEALREEVWRPFEQRARVLHHFGYLDFFAERVTERGRWL
                     ADLRTDRPLLVGESIGRGLFAPLDARRAAGLMAALASDPERDYGALALDDSLVKTLAE
                     FDRIAYEVGGVEWRHGLEPAPEINFSAAATAARWASGVEWKQLVSETRAEEGDLFRML
                     SRTGEALLQVSNLREAHPEAAATAARAAASVLREPVRSEANV"
     gene            5854..6909
                     /locus_tag="VF240_10320"
     CDS             5854..6909
                     /locus_tag="VF240_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175642.1"
                     /translation="MTELFEEFEVNREPRSKSLARTTVLSLAVHGVFIMLVLYGPTVH
                     AFWQLASMVTGAEYADEAYERGAVRERAVMISPHEKLYYPPGYLNPTLPPAPDAQVVE
                     EPTPTPTPRPRPTPKPRPTPAPTPDAASEVADKDKPGDDSALSDEEKKAKAEQEAKER
                     EALDRVAEQNKVKLPPKINSRPFKDMLVKWNGEYEKGRLNLNGTIAVTLEADRNEDGT
                     LTNMVMTGGSASDPALKELAKDVVATLSASRALEFLQGSRHLKLRLVLDRQKLNITVN
                     TEMDSAERASSMANVYGLGIIGMRWKKAGTDEGAAWKNTRVSAQDKTIVVTFDMPRET
                     AGSMLARQVGKAREGQQ"
     gene            6942..7730
                     /locus_tag="VF240_10325"
     CDS             6942..7730
                     /locus_tag="VF240_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175643.1"
                     /translation="MSTRMISDFLYRIAREREADARGSRRRRPVAALLRYAVLGAVAL
                     SILPASAAASLPTHAQDKSGTRADSEPRFVPERPAQTNAAADGVRRPPRVDTRPFKEL
                     LLKGKGLYDSGRLDLSRTIDLALVADREDDGSLVSIASAGAPLNDPLVFQFVKETVDT
                     ISQSRLLGFLDGTRRIRLDLSLDEQKLTITARTEVASEEYARKMASGYGALLAYARLM
                     ERERPSGVVWNHTGLMADGKQFAVTLEMSREQAGQLLLQLITPN"
     gene            7910..10750
                     /gene="secA"
                     /locus_tag="VF240_10330"
     CDS             7910..10750
                     /gene="secA"
                     /locus_tag="VF240_10330"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7175644.1"
                     /translation="MIDKFLTKVFGSQNQRYLKSLAPVVAEINALEPAIKALTDEQMR
                     ERIAQLRTQVIESIGDTRAKDDAGREARKRRTREALDAVLPEVFALTREASVRSTGMR
                     HFDVQLIGGMALHQGKISEMRTGEGKTLVATLPATLNALTGRGVNVVTVNDYLATRDA
                     EWMGRIYKFLGLTVGAIVNDLDDWERKDAYAADITYGTNNEFGFDYLRDNMKFDLATC
                     VQRDHHYAIVDEVDSILIDEARTPLIISGASDEATDKYYKADAVIPHLRKAELNAEGK
                     AGPGDYLVDEKQHSAVLTEEGVDRAENILGVGNLYEPSNMEMLHCVEQALKAHTLYKL
                     DHHYVVQDGEVIIVDEFTGRLMKGRRWSDGLHQAVEAKEGVKIERENQTLATITLQNY
                     FRLYDKLSGMTGTAETEAAEFEGTYKLEVALIPTNQPMVRKDNSDVIYRTLPEKWDAV
                     VEEIKELHEKGQPVLVGTVSVENSELISRRLHKAGIPHRDERGVMDFDDERREEVPAY
                     HYVLNAKHHEREAEIVAQAGRKGAITIATNMAGRGTDILLGGNPEFMARERLKRQEID
                     PDEATPEQFEEALEHAKHIVEEEHREVVGAGGLHILGTERHESRRIDNQLRGRAGRQG
                     DPGSSRFFLSLEDDLMRIFAGDKVKALMQRLGMEKGVAIESKMVSKRIESAQKAVEAR
                     NFETRKHLLQYDDVMNKQRETIYGLRRMLMEEPDQREYLLGEKGVARDLLADLTLQYL
                     NPEVAPDDWDVENYRVQLLSTYAFDPDAEGIDFENMGTQLIEETAWEKLKAHYAEKET
                     QIGEEAMRTYERIIMLNIIDAQWKDHLLAIDHLKQGIGLVGYGQKDPLVEYKKESFDL
                     FQAMLDRIDTITIRTLFNLQVVAEQAPEQLQQRRKSRPTRMSFTGPNQGATAAGEEAA
                     RTKTVTRDQPKVGRNDACPCGSGRKYKKCCGA"
     gene            10827..11936
                     /locus_tag="VF240_10335"
     CDS             10827..11936
                     /locus_tag="VF240_10335"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7175645.1"
                     /translation="MRLASILFASSLSLSSPHAPRAGAREAATPVGQTSAAQATQGPT
                     APEQAAVRDEIKSLLLAVGGERKKDADAWYRLGVAYQRAGEVAEARKAFKQAVKLRKR
                     FVAARVGVAYTYLAEKNYVEAEKEAKQAADLAIDTRDFRAYGFLSNIRLQRYRETSVR
                     ISAQADKVLATRPDDAEWRQLKAQALIGLTISESQQSMPDLSEPPPPPSPPPPPDEAK
                     AEAARERNLKLYKEAAEHLDAYLRLSPKAPDAAYQRAQLAALRFHVLSLEDGPAHGVF
                     KPKELTSKAVLLKRPEPGYTPEARAADISGMISARVLLGADGAVKHILIVKPLSHGLT
                     ERALDAARRIKFKPAVKDGRPVSQWVIVEYHFNIY"
     gene            11959..13170
                     /locus_tag="VF240_10340"
     CDS             11959..13170
                     /locus_tag="VF240_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175646.1"
                     /translation="MSSEVKSTNPVVQAILGGTAPQPARLAAARGLLPLAQEELIEVL
                     VALRASDDATITAAAEETLSQQEPAALAEIAASPEAAPSVLTYLAGRSDLERGIHESV
                     ALNTRTPDEAVTTLAGASTDGRVLEVISINQQRLIRAPAIIDAILANPARTPEAERRA
                     RETRREFFEKERGARQIADEMRARGMGAAAEFVESAESIGEAGGLTPEDAWLIAQHIE
                     VSDADVDDSWLPSGLFDELIEETDEQRGANVERVLNETAAEVADAAPERVALIRSIMF
                     MKVKDRVKLGLKGDREARNILIRDSNKTVSAAVIHNPKITDQEVEAIAAMRTVSDEVL
                     RLVALNRAWARSYPVIHNLARNPRTPLPTAMSILPRLQTRDLKGLIQNRNVSEAVRRQ
                     ALRLSSTRAGK"
     gene            complement(13258..13791)
                     /locus_tag="VF240_10345"
     CDS             complement(13258..13791)
                     /locus_tag="VF240_10345"
                     /inference="COORDINATES: protein motif:HMM:NF021503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HEX7175647.1"
                     /translation="MPSGRTHDLITFVLAPPTFLAVWVATGSLKLAAVVTCATLFGGL
                     MFGPDLDIQSRQYTRWGVFRFLWLPYKVAFRHRSRLSHGIILGTLIRVLYFTGAVAAL
                     TLLAVYLHARFVAGDAMPGVTELVSAWHAVAARATALGAGRDVLLAAFGGLWWGAAIH
                     TFADVMWSILRKSTEIF"
     gene            13975..14967
                     /locus_tag="VF240_10350"
     CDS             13975..14967
                     /locus_tag="VF240_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7175648.1"
                     /translation="MGKKSKRQIALEYAAARVILSGLGALPRPLAMAAGRVLGRAAYA
                     VAGGLRRTGERNLELALPELDERERRRILRGSFQSLGRQLGAFSRFPRETAESLREIV
                     EYDSEDVKLLDVARERGRGVIFLTAHLGAWELLCFAHSAFYNPIHFLVRPLDNPRVDE
                     LIEGMRTRFGNQPIDKGAAALTAMRLLRRGGTLGILADLNTQPREGVFVPFFGRLACT
                     TAGAATLALRTDSTVIPCCAPWDERRKKYVFRGGPVLELVRTGDAARDVELNTARFTA
                     AIEEQVRRFPDQWLWIHKRWKTRPEGEPDLYAKKIDATRAPFEPHTTSESAFKT"
     gene            complement(15435..16196)
                     /gene="fabG"
                     /locus_tag="VF240_10355"
     CDS             complement(15435..16196)
                     /gene="fabG"
                     /locus_tag="VF240_10355"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX7175649.1"
                     /translation="MSEEAKAFAGRVGLVTGATRGIGRAIALELARRGASVAFNYAQS
                     AEAAETLKGEIESAGVAVFASQCDVADTAGAAEMVKQVKDAFGRIDFLINNAGITRDQ
                     LILRMKEDDWDAVIDTNLKGAWNFSRAVLRPMLRQDGGGSILNISSISGVVGMAGQSN
                     YSASKAGMIGLTKSLAREVASRNVSVNALALGLVSTDMSGALDDAYREKLLEQIPMKR
                     FAQPEEVARIACFLLSDDARYITGQVVQIDGGMAM"
     gene            16510..17268
                     /locus_tag="VF240_10360"
     CDS             16510..17268
                     /locus_tag="VF240_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7175650.1"
                     /translation="MKLIILGAGYATRLYPLTLDRPKPLLGVAGKPMIEHVLDNLSAV
                     GDIDHVYVVTNAKFAGHFQEWAERYRDDRGKADFTIVNDESTDDSNKLGAIGDLHLVL
                     TRERIDDDIIVVAGDNLFSHSLEKFGEFCREKKAPVLAVYDVGDLEEIKKYNAIDVDG
                     AGRITFFEEKPARPKSTLTGIALYYYPKSALPLIRQYVEEGNNPDQPGRLVQWMYERT
                     PFYTWRVPGIWYDVGSKETLEEANRIFSDPALKG"
     gene            17359..18654
                     /gene="serS"
                     /locus_tag="VF240_10365"
     CDS             17359..18654
                     /gene="serS"
                     /locus_tag="VF240_10365"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7175651.1"
                     /translation="MLDLNFVRENFERVRASLEARAFPVAVLDDFARLDSERRRVIAE
                     SDQLNAERNAAAREIGALMKEGKKEEAEGRRGRAAELKDRISGLDRERDEAERRMQEL
                     LSGVPNVQHESVPTGTGEDDNVEARRWGNRPELDFEPQDHVDLGVSLGILDLERATKI
                     AASRFSILFGAGARLERALIQFMLDVHTEEHGYTETLPPFIVNAAALYGTTQLPKFEQ
                     DLFKLTDERGLYLSPTAEVPVTNIHREEILEAAELPKKFVAYTPCFRSEAGSYGRDTR
                     GIIRQHQFDKVELVKLALPEDSYAELEGLTRDAETILQRLGLHHRTVTLSTGDIGFGS
                     AKTYDIEVWLPSQNTFREISSCSNYESFQARRAQIRFRRTPGAKPEYVHTLNGSGLAV
                     GRTWVAILENYQQQDGSVVIPEVLRPYMGGMEKIEGRRQ"
     gene            complement(18937..20742)
                     /gene="sppA"
                     /locus_tag="VF240_10370"
     CDS             complement(18937..20742)
                     /gene="sppA"
                     /locus_tag="VF240_10370"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX7175652.1"
                     /translation="MSGTRKLILIILGIVMALVVVVGLGVALLFYSLREHEPNVRDHS
                     VLVLKVAGALPDYANDDPFAARFLGAETNSLTSLLTQLKKAKADKRVDAVLLDIDFVG
                     VGWAKAAEIRDAIADFRSSGKPAYGYMEYGADKELYIATACDRVYVAPAGDLFINGLA
                     AQAMFFKGSLDKLGVEMDVHQIGKYKNAPDSYTRKDMSDAQREVINAILDDTFNRYVE
                     AVARDRKKSPEDVRALIDDAPITARRAQEAGLIDGALVREEVENELKKRVGGYKDDEK
                     LHTVSLSEYKRVSADSLGLNQGERIAVIYASGVINSGHSNDGSFGGEQQVGSDTVVRA
                     INDARDDKGIKAIVVRVDSPGGGIYPSDMIWRALEAAKQKKPVVISMSDLAASGGYYI
                     AAGANRIVAQPGTITGSIGVFGGKPVISGFYDWLGISSEYVLRGKQAGMFRETEKFTP
                     DERARFEALIEHFYWDEFIPKVAEGRKRDREYVHSIAQGRVWTGQQAQERGLVDEFGG
                     LDRAVEIAKELAGIPADKGVRRVVFPAPRTFFQQLFDEDSDEDKSSLQARRQEQAVIN
                     SLPEDLRRAFAYARLFDQMKHGKGLALMPFELRIE"
     gene            complement(20928..>21624)
                     /locus_tag="VF240_10375"
     CDS             complement(20928..>21624)
                     /locus_tag="VF240_10375"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX7175653.1"
                     /translation="DAGANRYRLLRGLDARKDQTYFLWELTQAQLARSMFPLGEMSKP
                     EAREAARGANLSGVAEKAESQEICFVPDGDYAGFIDRYLAAKGRDDAPGAGEIVDTEG
                     RVVGTHGGVQRYTVGQRRGLGLARELPVYVVRVDAEANRVVIGGAEELLSQEFTAAGV
                     NWVAFDEPAGSVRAEVRVRYRHQESPATVTPLAEGRARVRFDEPQRAITPGQATVFYR
                     GEEVVGGGWIVKK"
BASE COUNT         3928 a         7322 c         6967 g         3407 t
ORIGIN      
        1 gcgttgagcg tggtcagcgg caacgactcg aaggccgggg ggaactggcc ggagcgcatg
       61 ccgtcctgaa gcatccgcgc cagacgaaaa tggtaatagc cgtcgtagtc gccgcagcag
      121 atcgcctgcg tggaaaactg cgtccaccaa aacgagaaac cgacgaggag cgcgccgacg
      181 acaaagagga tggtgcggtc gcgcacgcca gtctcgacgc gcacccgctc ggccgggaga
      241 ctcgtcggcg ccgcgctctc cgcctcgacg cgctctccga taccgatatc ttccatgact
      301 agattgagct acctcacgtc aggcgcaccg gcttcggcgc aggggcttgg aaattgacag
      361 acgaacataa cacggaggcc cgccgctcat gaagcgcgcg ggccgtccga ggttgctctc
      421 ttaaggagcg aagttactgc ggcgccatgc cgcaacagag ggggccgagc atggcattac
      481 actcgtgcag ttgataccac agaccgctgg cgtaaacgcc ggaggcgaac gtgagcagcg
      541 cggcaaacag tcgagacagc agcggtctca cccgtctgcg cgataacaca agctcacatc
      601 ttccgggcat gtttttctct cttaccttcc aaccgggacg gcggcctcgt gctccgcgcc
      661 gatctctttg agacgcgcga cgacctgctc gatgagccag ttcggcgtcg aggcgccggc
      721 cgtgacgccg acgcgctccg cgccgcgcac gtcttcgggg tcgatctcct cgggcgtctc
      781 gatgaggaag ctgcgcgggc actgctcctt gcagacggag agcaacttga tgctgttcga
      841 tgagtgcttg ccgccgatga tgtagaagac ctcgacctct ccggccagtg cgcgggcggc
      901 gtcctgccgg tcgcgcgtcg ccgagcagat ggtgttgacg acctgcggct cggcgtcgga
      961 cttggcgcgc acggcctcag ccgcttcgag gaaggtgctc agcttgagtg tggtctgcga
     1021 gacgacgagg ggggcgtgca gaaacggaag gagcgccact tcgctcgcgt cgcggacgac
     1081 gtgagcgttc tgcggcgcgt agcccttgac gccgatcatc tcggggtggt cggggctgcc
     1141 gaccacgacc acgtcgcgcc cctcggcggc ggcgcgctgg gccagtattt gcaccttcgt
     1201 gacgaacggg caggtggcgt caacgacctt cgtcgcgcgc gcctccaatt cccgttgcac
     1261 ctctggcgtt acgccgtgcg cgcgaatgac ggccgtcacg cccgactcga cctcggcggg
     1321 cgcgtcgatg gtgcggacgc cctccgattc gagccgccca atctcctgag tgttgtggat
     1381 gagcggcccc agcgtccgca cggtcgcgcc ctcttgcagc gcctcctcca ccatctccac
     1441 ggcgcgttcg acgccgaaac agaaaccgaa ctcttcagct aagagaactt gcatgattat
     1501 tcagagcgat tagctgttag cggtcagcta aagacaaaaa gctaagggct aacagctaac
     1561 agctaatcgc taatagcttc tttcagattc ccccggccgg gccttccgct gtcggcggcg
     1621 tggtcgggtc ggtgtagctg gcgctgcgtt ggcgccggct gttccactcc tcgcgcaggc
     1681 ggtcgccggc ggcgaggctt tcgtccaccc agctcatgtt cttgtcggcg aaccagcgca
     1741 cgccgcccca gaagttcttc tcgaactcgc cgagcttgta ggccgcgtcg cgcggaagaa
     1801 cggcgaacga ggcgtcgcac aaaaagcgca gggcgtcgca cacggggttg ccggttttgt
     1861 agcgagggtc gttgttgtca gagcagttca taatttcgta aaacctccta cccgtgacat
     1921 acggccaaac cggcgcccgc gttcccacgc cgtcaatctt aacacggccg ggagtgagca
     1981 gtaagcggtg agcagtgagc agtaaagaca gtttgcgcct tataaaccgc tccccgcttg
     2041 ctgctcactg ctcaccgctt actgctcact ccccgatggc ctctttgatc tccggccaca
     2101 tcttcacggc ccagaactcg tagccttctt cggacgggtg gaagccgtcc ggggagaaaa
     2161 attcggggtg tgtagggatc aactcgtggc tgaaggcgta ggcgtcgagg agccggaggc
     2221 cgtgggcgcg ggcgacggcg gccagcgatg cgttgaaggt ctggatgcgt tggcttgccg
     2281 cggcgcgcag gaagacgggg acgaccgggg cgagggagat gtcggggatg ttggcgacga
     2341 ccagcggcgc cggcgtcgcc tcgcgcaggc gggtgacgat ctcctcgaag ttgcgcgcga
     2401 actgctcggg cgcgaccatg cgtgtcaggt cgttgatgcc gatgccgagc gtgacgagcg
     2461 tggggcgctc ctgccggacg cggcccacct gcgcgcgcag cacgtcggcc gtcgtcgcgc
     2521 cgctcacgca caaattgacg agacgcgagc cgggccgcgc gcgctccaga cgctcgaaca
     2581 gacgcgcgac gtacccgccg ccattgcgcg ccccgacgcc gacgcccgtc gaatcgccga
     2641 gcgcgacgta gacgacgggc ggcttctcct cccgactcat actcacctca tctgcggagg
     2701 gatgaaggat gagggatgag ggatgaacaa gacaggacta atccgccttc atccctcatc
     2761 cttcatccct ccatcttaga cgttcgcctc cgagcgcacc ggctcgcgca gaacggacgc
     2821 ggcggcgcgc gcggccgtgg ccgccgcctc ggggtgcgcc tcgcgcaggt tcgagacctg
     2881 aagcagcgcc tcgcccgtgc gcgagagcat gcggaagagg tcgccctcct cggcgcgcgt
     2941 ctccgagacg agctgcttcc attcgacacc cgaggcccag cgcgcggccg tcgccgccgc
     3001 cgagaagttg atctccggcg ccggctccag cccgtgtcgc cactcgacgc cgccgacctc
     3061 gtaggcgatg cggtcgaact cggcgagcgt cttcaccaga gagtcgtcaa gcgcaagcgc
     3121 gccgtagtcg cgctcggggt cggaggccag agcggccatg aggccggcgg cgcgacgcgc
     3181 gtcgagaggg gcgaagaggc cgcgcccgat tgactcgccg acgaggagcg gccgatcagt
     3241 tcgcaggtcg gcgagccagc ggccgcgctc cgtgacgcgc tcggcgaaga agtcgaggta
     3301 gccgaagtgg tgcagcacgc gcgccctctg ctcgaagggc cgccacacct cctcgcgcaa
     3361 agcctcggcc tgtcgctcgg cccgctccaa cccttcggcc tccttgacga ccgaccacag
     3421 aatctcctcg cagcgcgtcc gctcctcacc ctcggcgacg ccggccgaga gcgactcgat
     3481 cagctcgttg attaaatcga cggccccgtc cgcgtcggcg cgcgagtcgc gcaggcgcgg
     3541 ctcgctaatc ggctcggcct tgccacgctt cacgctctcg aaagcctcgt cgcgcgccga
     3601 ctcgtcgcgc gcgaagatgg tcgcgtagac gcggccgatg cgttcgagcg ccacggaggc
     3661 ccggcgtccg tcctcgcggt agccgacgag gccgtcggcg cgccgctcgg tgacgaagac
     3721 gagccggcgt ccgctcctcc cgacgccgac gacgcggccg ggcacgacct ccttgtcgag
     3781 ccagcgcagc gtcgcctcga agcgcgtgtg cggcgcgcct tccagcaagc gcgagcgcgc
     3841 gctcgccagc cgttcgagtc cgcgcgcgac ctccggcggc agttggcagc cagcctcccc
     3901 gagccggtcg cgcatccttc cttcggtctc ggcgcgctcg cgctccagac ccgtcagtcg
     3961 ctcgccggcc tcgcgttgcg agaagctgcg ctcggcgatg tcgcgcacct gcgagaaggt
     4021 gccgtaggcg tcgaggaggt tgaggagcgt ggtgtaagtg gcgcggaact ggctctccaa
     4081 agggtcgggc ggcgaatgga gcaacgcggc gagcttgtgc gggtcttgat gctggcccgg
     4141 tgcggcgacg atgaagccga cgcggtcgcg cccgcgccgg ccggcgcgac ccgtcatctg
     4201 ttgcagctcg gaggcggtga gcacgcgcca gccgcgtccg gttcgcacgt cgatgttggc
     4261 gagcacgacc gttcgggcgg ggaagtccac gccggcggcc acggtcgccg tcgcgaagat
     4321 ggcgtcgagc agacctgcgc tcatcaactt ttcaatcgcg agcttccacg ccggcaggtg
     4381 tccggcgtgg tgcgaagcga cgccgccgcg cacgaccatg tcccagtgcc ggtgccggcg
     4441 cacctccggg tattgctcga cgagttcgtc gagtaagacg cgccgcgcct cgcgcctgtg
     4501 cgtgttgttg ttggcgcgtg gcgcgtgggc ggactcgacc gcggcctcgt cgcagcgccg
     4561 gcgcgtgggg aggaagacga tggccggcag caggtcgtag gacgagagcg cggtgagcag
     4621 tgtcgagggc ggcatctcgg gcatgcgcgg acgctggggc gcgccgccgc gtgcgccgaa
     4681 gcgcgtgtgc ggctcggggc ggcgctcgct ccgggaggtc tgcatgaagc gggcgatctc
     4741 ggagttgaag tggttgcgct cgtcgaagag cggcgcgatt tgtccgtcgg ggaagaggaa
     4801 tgcggcgcgt aaggggacgg ggcgcgcgcc gggccgcgca acgacgcggc acgcgtgccc
     4861 gcgcacctcg gcgatccact cggcgaactc ctcggcgcgc cccaccgtcg ccgagagcag
     4921 aagcatccgc acgcgcggcg gcgtgaggat gatggcctcc tcccagacgt gcccgcgctc
     4981 ctcgtcggcg aggtaatgcg cctcgtcgag gatgactaaa tcgacatcga cctgccggcc
     5041 gccgcgtagc gcgtcgaaga gctggttacg gtagacctcc gtggtgccga cgacgagcgg
     5101 cgcctctgag ttctccttgc ggtcgccggt gagcacgccc accgtcccca cgccgaactc
     5161 cgcggagaac tcctgaaact tcgagttggt cagcgccttc agcggactcg tgtaccacgc
     5221 gcgccggccc tgctccagaa gccgcctgat ctcctcgcgc gcaatccacg tcttgccgga
     5281 gccggtgggc gccgtgacga gcacgtcctc gtgttcgagc gcggcgaggg cttcgagctg
     5341 aaacgggtca gggacgaagg gcgcgggcgc gggcgcgccg atgccttcga gcagttgccg
     5401 gacggggcgc gtgcgcgacg cgcggccggc gacgtgtgag acctgcgccg tctcggcggg
     5461 cgacgctgcg gggcgcgtag tcgcccgccc acgcccgccg ccgtcacgac gcagggcgtc
     5521 gccgcgcgtc gcgccgtcac gcgccccgcg ctcgtcctgt gcgccgccgg acgtgcgtcc
     5581 gccccggccg ccccgtttga gtccgcccct cggcccacgc cccccgccgc ctctttgtcg
     5641 ccccatacgg ccattctaac gcagtgagca gtgagcagta agcagtgagc agtcagaagg
     5701 catcacttgt cttcactgct tactgctcac tgctcactgc tcaccatctt gtgttacttt
     5761 tacgtgtcgg ctcgcaacgc cgggggcggc gtccgcgtct aaaattctcg aacgccgggc
     5821 gttgtgcggc gtataacggg gaagtcgaaa cagatgaccg aacttttcga ggaattcgag
     5881 gtcaaccgcg agccgcgctc gaagagcctg gcgcgcacca ccgtgctctc gctcgccgtg
     5941 catggcgtgt ttatcatgct cgtgctctac gggccgaccg tccacgcctt ctggcagctc
     6001 gcctcgatgg tgacgggcgc cgagtacgcc gacgaggcat acgagcgcgg cgccgtgcgc
     6061 gagcgcgccg tgatgatctc gccgcacgag aaactctact acccgcccgg ctacctcaac
     6121 cccactctgc cgcccgcgcc cgacgcgcag gtggtcgaag agccgacccc gacgccgacg
     6181 ccgaggccga ggcccactcc aaagcccaga ccgacgccgg ccccgacgcc cgatgccgcc
     6241 tcggaagtcg ccgacaaaga caagcccggc gacgacagcg cactgagcga cgaggagaag
     6301 aaggcgaagg cggagcagga agcgaaggag cgcgaggcgc tcgaccgcgt ggccgagcag
     6361 aacaaggtca agctcccgcc gaagatcaac tcgcgcccgt tcaaagacat gctggtgaag
     6421 tggaacggcg agtatgagaa ggggcggctg aatctgaacg gcaccatcgc cgtcaccctc
     6481 gaagccgacc gcaacgaaga cggcacgctc accaacatgg tgatgacggg cggctcggcg
     6541 tcagacccgg cgctcaagga gctggccaag gacgtggtcg ccacgctcag cgcgagccgc
     6601 gcgctcgaat tcctgcaagg ctcgcgacac ctcaagctgc ggctcgtcct cgaccggcag
     6661 aagttgaaca tcaccgtcaa caccgagatg gactcggccg agcgcgcgtc gagcatggcg
     6721 aacgtttacg gcctcggcat catcggcatg cgctggaaaa aagccggcac ggacgagggc
     6781 gcggcatgga agaacacgcg cgtctccgcg caggacaaga ccatcgtcgt gaccttcgac
     6841 atgccgcgcg agacggccgg cagcatgctc gcccggcagg tcggcaaggc gcgcgaaggc
     6901 cagcagtaac gtagccagcg cgacagcgcg cccctcgact gatgtcaacg agaatgatct
     6961 ccgacttcct ctacagaatc gcccgcgagc gcgaagcgga cgcgcgcggc tctcgccgtc
     7021 gccgccccgt cgccgcgctc ctgcgctacg ccgtgctcgg agccgtggcc ctcagcatcc
     7081 tgcccgcctc ggccgccgcc tcgctcccga cgcacgcgca ggacaagtcg ggcacgcgcg
     7141 ccgactccga gccgcgtttc gtgcctgagc gtccggcgca gacgaacgcg gccgccgatg
     7201 gcgtgaggcg tcccccccgc gttgacacac gccccttcaa ggaactcctg ctcaagggca
     7261 agggactcta cgactcgggc cgcctcgacc tgagtcggac aatcgacctc gccctcgtag
     7321 ccgaccgcga agacgacggc tccctcgtca gcatagcgtc ggcgggcgcg ccgttgaacg
     7381 acccgttggt gtttcagttc gtcaaggaga cggtcgacac aatcagccag agccgcctcc
     7441 tcgggttttt ggatggcacg cgccgcatcc gcctggatct ctcgctcgac gagcagaagc
     7501 tcaccatcac ggcgcgcacc gaggtcgcat cggaagagta tgcccggaag atggccagcg
     7561 gctacggcgc tctgctcgcc tacgcgcggc tcatggagcg cgagcggccc tcgggcgtcg
     7621 tctggaacca cacgggcctc atggcggacg gcaaacagtt cgcggtgacg ctcgaaatgt
     7681 cgcgcgaaca ggccggccaa ctcctcctcc agctcatcac accgaactaa cccctccgcg
     7741 catggcgcac gccggcggcc ccgaaaaggg cctcgcttgc cccgcgtccc acgccccgcg
     7801 tacaatccaa agttgcgccg acacgcgcaa ttgttcgccg tcgcggcccc gactcttcac
     7861 aggaagcggg ccgcgcgagc cgtctctttt tcattacagg attaaagcaa tgatcgacaa
     7921 gtttctgacc aaagtcttcg gcagccagaa ccagcgttac ctgaagagcc tcgcgcccgt
     7981 cgtggccgag atcaacgcgt tggagccggc catcaaggct ctcaccgacg agcagatgcg
     8041 cgagcgcatc gcacagctcc gcacgcaggt catcgagtcc atcggcgaca cgcgcgcgaa
     8101 agacgacgcc ggccgtgagg cgcgtaagcg ccgcacgcgc gaagcactcg acgcagttct
     8161 gcccgaggtc ttcgccttaa cgcgcgaggc gtcggtgcgc tcgacgggga tgcgccactt
     8221 cgacgtgcag ctcatcggcg gcatggcgct ccatcagggc aaaatctccg agatgcgaac
     8281 cggcgaaggc aagacgctcg tcgccacgct gcccgccacc ttgaacgccc tcacgggccg
     8341 cggcgtcaac gtcgtcaccg tcaacgatta tctcgccacg cgcgacgccg agtggatggg
     8401 ccgcatctac aagtttttgg gcctcaccgt cggggcgatt gttaacgacc tcgacgactg
     8461 ggagcgcaag gacgcctacg ccgccgacat cacctacggc accaacaacg agttcggctt
     8521 cgactacctg cgcgacaaca tgaagttcga cctcgccacc tgcgtccagc gcgaccacca
     8581 ctacgccatc gttgacgagg ttgactccat cctcatcgac gaggcccgca cgccgctcat
     8641 catctcgggc gcgagtgacg aggccaccga caagtattac aaggccgacg ccgtcatccc
     8701 gcacctgcgt aaggccgagt tgaacgcgga gggcaaggcc ggccccggcg actacctcgt
     8761 tgacgagaag caacactcgg ccgtgctcac ggaagagggc gttgaccgcg ccgagaatat
     8821 tctgggcgtc ggcaacctct acgagccgtc gaacatggag atgctccact gcgtcgagca
     8881 ggcgctcaag gcgcacacgc tctacaagct cgaccaccac tacgtcgtgc aggacggcga
     8941 ggtgatcatc gtcgatgagt tcacgggccg actgatgaag ggccgccgct ggtcggacgg
     9001 tctgcatcag gccgtcgagg ccaaggaggg cgtcaagatc gagcgcgaga accagacgct
     9061 cgccaccatc accctccaga actacttccg cctctacgac aagctctccg gcatgacggg
     9121 cacggccgag accgaggccg ccgagttcga gggcacatac aagctcgaag tggccctcat
     9181 cccgaccaat cagccgatgg ttcgcaagga caactcggac gttatctacc gcacgctccc
     9241 cgagaagtgg gacgccgtcg tcgaggagat caaggaactc cacgagaagg gacagcccgt
     9301 cctcgtcggc accgtctcgg tcgagaactc cgaactcatc tcgcgccgct tgcacaaggc
     9361 cggcatcccg caccgcgacg aacgcggcgt gatggatttc gacgacgagc gccgcgaaga
     9421 ggtgcccgcc tatcactacg tcctcaacgc caagcaccac gagcgcgagg ccgagatcgt
     9481 cgcgcaagcg ggccggaagg gcgctatcac catcgccacc aacatggctg gtcgcggcac
     9541 cgacatcctc ctcggcggca accccgagtt catggcgcgc gaacgcctca agcgacagga
     9601 gatcgacccc gacgaggcca cccccgagca gttcgaggag gcgctcgaac acgctaagca
     9661 catcgtcgag gaggagcacc gcgaggtcgt cggcgcgggc ggcctccaca tcctcggcac
     9721 cgagcgccac gagtcgcgcc gcatcgacaa ccagttgcgc ggtcgcgccg gccgtcaggg
     9781 cgaccccgga agcagtcgct tcttcctttc gctcgaagac gacctgatgc gtatcttcgc
     9841 cggcgacaag gtcaaggcgc tgatgcagcg tctcgggatg gagaagggcg tggccatcga
     9901 gtcgaagatg gtctcgaagc gcatcgagtc ggcgcagaag gcggtcgaag cgcgcaactt
     9961 cgaaacccgc aagcacctgc tccagtacga cgacgtgatg aacaagcagc gcgagaccat
    10021 ctacggcctg cgccgcatgc tcatggaaga gcctgaccag cgcgagtatc tgctcggcga
    10081 gaaaggcgtg gcccgcgacc tgctcgccga cctcaccttg cagtatctca accccgaggt
    10141 cgcgcccgac gactgggatg tggagaacta ccgcgtgcag ctcctctcca cctacgcctt
    10201 cgacccggac gccgagggga tcgacttcga gaacatgggc acgcagctca tcgaggagac
    10261 ggcgtgggag aagctcaagg cccactacgc cgagaaggag acgcagatcg gcgaagaggc
    10321 gatgcgtacc tacgagcgca tcatcatgct caacatcatc gacgcccagt ggaaggatca
    10381 cctcctcgcc atcgaccacc tcaagcaggg catcggcctc gtcggctacg gccagaaaga
    10441 cccgctcgtc gagtacaaaa aggagagctt cgacctcttc caggccatgc tcgaccgcat
    10501 cgacaccatc accatccgca cgctcttcaa ccttcaggtc gttgcggagc aagcgcccga
    10561 gcagttgcag cagcgacgca agagccgccc cacgcgcatg agcttcaccg gccccaatca
    10621 gggcgcgacg gccgccggcg aggaggccgc gcgcaccaag accgtcacgc gcgaccagcc
    10681 caaggtcggc cgcaacgacg cctgcccctg cggctcgggc aggaagtaca agaagtgctg
    10741 cggggcctga gcgaagcaca agaactgcgg cggcgcgtcg ttcacgcgcc gccgccttaa
    10801 ccttcgtgcc agagggcact gaccacatgc gcctcgcgtc catcctcttc gcctcgtctc
    10861 tcagtctttc gtccccccac gccccgcgcg ccggcgcgcg cgaagccgcc actcctgtcg
    10921 ggcaaacgag cgccgcacag gccacgcaag ggccgacggc ccccgaacag gcggccgtcc
    10981 gcgatgagat aaaatcgctg ctcctcgccg tgggcggcga gcgaaagaaa gacgcggacg
    11041 cgtggtatcg cctcggcgtc gcgtaccagc gcgccggcga agtggccgag gcgcggaagg
    11101 ctttcaagca ggccgtcaag ctgcggaagc gtttcgtcgc cgcgcgggtc ggcgtggcct
    11161 atacctatct tgccgagaag aactacgtcg aggcggagaa ggaggctaag caggccgctg
    11221 atctggccat agacacaaga gactttcgtg cctacggctt cctttccaac atcaggctac
    11281 agcgttaccg agagaccagc gtcaggattt cagctcaggc cgataaggtg ctcgccacaa
    11341 gacccgacga cgccgagtgg cgccaactca aggcgcaggc gctcatcggc ctgaccatct
    11401 ccgagagtca gcagagcatg cctgacctgt cagagccgcc accgcctccg tcgccgccgc
    11461 cgccgcccga cgaggctaaa gcggaggccg cgcgggagcg caacctgaaa ctgtacaagg
    11521 aggccgccga gcatctggac gcatacctcc gcctgtcgcc gaaagcaccc gacgccgcct
    11581 atcagcgcgc acaactcgcg gcgctccgct tccacgtcct gagcctggag gacggcccgg
    11641 cgcacggcgt cttcaaaccg aaggaactca cgtcgaaggc agtcctcctg aaaaggccgg
    11701 agccgggata cacgcccgaa gcgcgcgccg ccgacatcag cgggatgatc agcgcgcgcg
    11761 tgctgctcgg cgccgacggc gccgtcaaac acattctcat cgtcaagccg cttagtcacg
    11821 ggctgaccga gcgggctctc gacgcggcgc gccggattaa attcaagcct gcggtcaagg
    11881 atggacgccc tgtctcgcag tgggtcatcg tcgagtacca cttcaacatc tactgagggc
    11941 aacttcccgc gggcctgtat gagcagcgaa gtcaagtcta caaatccggt cgtgcaggcc
    12001 attctcggcg gcacggcgcc gcagccggcg cgtctggccg ccgcgcgcgg actcctcccg
    12061 ctcgcgcagg aagagttgat cgaagtgctc gtcgcgctgc gcgcaagtga cgacgcgacc
    12121 atcactgccg ccgccgaaga aacgctctcg cagcaggagc cggcggcgct cgccgaaatc
    12181 gccgcttcgc ccgaagccgc gccgagcgtc ctcacatatc tcgccggccg ctcagacctt
    12241 gagcgcggga ttcacgagtc ggtcgcgctc aacacgcgca cgcccgacga ggccgtcacc
    12301 accctcgccg gcgcttcgac cgacggccgc gtcctcgaag tcatctccat caaccagcag
    12361 cgcctcattc gcgcgcccgc gattatcgac gccatccttg ccaaccccgc gcgcacgccc
    12421 gaggccgagc ggcgcgcgcg cgagacccgg cgcgagttct tcgagaagga gcgcggggcg
    12481 cgccagatcg ccgacgagat gcgcgcgcgg gggatgggcg cggcggccga gttcgtcgag
    12541 agcgcggagt caatcggcga agccggcggc ctcacgcccg aggacgcctg gctcatcgcg
    12601 cagcacatcg aggtctccga cgccgacgtg gacgactcgt ggctcccgtc ggggcttttc
    12661 gatgagttga ttgaggagac ggacgagcag cggggggcga acgtcgagcg cgtgctcaat
    12721 gagacggcgg ccgaagtggc tgacgccgcg cccgagcgcg tggcgctcat ccgcagcatc
    12781 atgttcatga aggtcaaaga ccgcgtcaag ctcggcctca agggcgaccg agaggcgcgc
    12841 aacatcctca tccgcgactc caacaagacc gtctcggcgg ccgtcatcca caaccccaag
    12901 atcaccgacc aggaggtcga ggccatcgcc gcgatgcgta ccgtctccga cgaggtgctg
    12961 cgtctcgtcg ccctcaaccg cgcgtgggcg cgctcctacc ccgtcatcca caacctcgcg
    13021 cgcaaccccc gcacacccct tccgacggcg atgagcatcc tcccgcggct ccagaccaga
    13081 gacctcaagg gcctcatcca gaaccgcaac gtctccgagg ccgtccgccg acaggcgctg
    13141 cgcctctcca gcacccgcgc gggaaagtga aagagagtga gcagtgagcg gtaagcagtg
    13201 agcagtagga agaggacgct caaccttact gctcactgct taccgctcac tgctcactca
    13261 aaagatttcc gtgctcttac gcaggatcga ccacataacg tcggcgaagg tgtggatggc
    13321 cgcgccccac cacaggccgc cgaaggccgc gagcaggaca tcccggccgg cgccgagggc
    13381 cgtggcgcgc gcggccacag cgtgccacgc cgagaccaac tcggtcacgc cgggcatcgc
    13441 atcccccgcg acaaagcgcg cgtgcaggta aacggcgagc agcgtgagag cggcgacggc
    13501 gccggtgaag tagagcacgc ggatgagcgt gccgaggatg atgccgtgag agagacgcga
    13561 gcggtggcgg aaggcgacct tgtagggcag ccacaagaag cggaacacgc cccagcgggt
    13621 gtactgccgc gactgtatgt ccagatcggg gccgaacatc aaccccccga acagcgtcgc
    13681 gcacgtcacg accgccgcca gcttcagact acccgtcgcc acccacaccg ccagaaaagt
    13741 cggcggcgcg aggacgaacg taatcaggtc gtgcgttctt cccgaaggca taacaagcag
    13801 aggtcagagg tcagaggtca gaggttagag gtcagttaaa ggagaagtgg tttttactga
    13861 cctctgacct ctaacctctg acctctgctt tatagcatgc gcgcggcggc ggcgtcagcc
    13921 gtttgcctgc gtgcgggggg cgggttaaac tgtgcctcct ctccggtggc actcatggga
    13981 aagaagagta aacggcagat agctttggag tacgcggcgg ctcgcgtcat cctctcgggt
    14041 ctcggcgcgc tgccgcgccc gctcgcgatg gcggcggggc gcgtcctcgg ccgcgccgcc
    14101 tacgccgtgg ctggcggcct gcgccgcacg ggcgagcgca accttgagct ggccctcccc
    14161 gaactggacg agcgggagcg gcgacgaatc ctgcgcggct ccttccagag tctcggcagg
    14221 cagttggggg ccttcagtcg cttcccgcgc gagaccgccg agtcgctccg cgagatcgtc
    14281 gagtacgaca gcgaggatgt gaaactgctc gacgtggccc gcgaacgcgg gcgcggcgtt
    14341 atcttcctca cggcccacct cggcgcctgg gaactcctct gcttcgccca ctcggccttt
    14401 tacaacccca tacacttcct cgtccggccg ctcgacaacc cccgcgtcga tgagcttatc
    14461 gagggcatgc ggacgcgctt cggcaaccag cccatcgaca agggggccgc ggccctgacg
    14521 gccatgcgtc tgctgcgccg cggcggcacg ctcggcatcc tcgccgacct caacacgcag
    14581 ccgcgcgagg gcgtcttcgt ccccttcttc ggtcgcctcg cctgcaccac ggccggcgcc
    14641 gccaccttgg cccttcgcac cgactcgacc gtcatcccct gctgcgcccc ctgggacgag
    14701 cgccgcaaaa aatacgtctt ccgcggcggc cccgtcctcg aactcgtccg cacgggcgac
    14761 gccgcgcgcg atgtcgagtt gaacaccgcc cgcttcacgg ccgccatcga agagcaggtc
    14821 agacgtttcc cagaccagtg gctctggatt cacaaacgct ggaagacacg ccccgaaggc
    14881 gagccagacc tctacgctaa gaaaatcgat gcgacccgcg ccccatttga accccacacg
    14941 acaagcgaaa gcgcattcaa gacttaaccc ccgcccctta acaactcacc ttagctccat
    15001 gttatcaggt gattaactgt cagtgccagt ctgttgcgaa agtgtgtaga aactttttca
    15061 ttttgtgctt gacagctcac aaaaagtcgg tataatttca cacacgttaa cgaagcaaag
    15121 acattttcga ttgatagtgc ttcgaagttg cttgaacggg tcggtctgtt ggggttcatg
    15181 caatctctcc gggctggggc aaagttagat ttattacgtc cccacgcgag gttgtgaagt
    15241 accaagcagt tcttatttcg cccatcccaa ctaaccacca aaaacaccgg atcgttcgta
    15301 tgtgacgatc tccagcacca actgacgcca cccccgaatc tccgagcctg cccaaaagtt
    15361 tcagccccgt gagtcttacc ccaagactcg cggggctcct tcattttagc taacagttaa
    15421 tagcagaccg cttttcacat cgccatgccg ccgtcgatct ggacgacctg gccggtgatg
    15481 tagcgcgcgt cgtccgagag caggaagcag gcgatgcggg cgacctcttc gggttgggcg
    15541 aagcgtttca tggggatttg ttcgaggagt ttttcgcggt aagcgtcgtc gagcgcgccg
    15601 gacatgtcgg tggagacgag gccgagggcg agggcgttga cggagacgtt gcggctggcg
    15661 acctcgcgcg cgagcgactt cgtcagcccg atcatcccgg ccttcgaggc ggaatagttc
    15721 gactgcccgg ccatccccac cacgccgctg atcgaagaga tgttgaggat ggagccgccc
    15781 ccgtcctgcc gcagcatggg gcgcaggacg gcgcgcgaga agttccaggc gcccttgagg
    15841 ttcgtgtcga tgaccgcgtc ccagtcgtcc tccttcatgc ggaggatgag ctggtcgcgc
    15901 gtgatgccgg cgttgttgat gaggaagtcg atgcggccga aggcgtcctt cacctgcttg
    15961 accatctcgg ccgcgcccgc cgtgtccgcc acgtcgcact gcgaggcgaa gaccgcaacg
    16021 cctgccgact cgatctcgcc cttcaatgtt tcggcggctt cggcgctctg cgcgtagttg
    16081 aaggcgaccg acgcgccgcg ccgcgccagc tccagcgcga tggcccgccc gataccgcga
    16141 gtggcgcccg tcaccaggcc cacgcggccc gcaaaggctt ttgcttcttc actcaaaagt
    16201 tgtcttcctc cgttggcggc gtcgggcgtc gcttacggcc cgccaccgag ataacgcgcg
    16261 gatgacgaac ggctctgacg cgcgcaaaac gggcgcaata taacagagct tcaggccacg
    16321 cggaagcgcg cgccgctctt cgcttttagt cgcaccgttt gcccacggca ggtttttatt
    16381 gtaaagattt tgtcggaact cattcgccga actgacgaag gcccgagccg aacgcgccgt
    16441 gcgtggtgcg tctgaatctt ttcaacggct gaaatctcaa ggccgatgcg gcacggcaaa
    16501 aggaaggtca tgaaactaat aattctgggg gccggctacg ccacgcgcct gtacccgctg
    16561 acgctcgacc ggccgaagcc gctgctcggc gtcgccggta agccgatgat cgagcacgtg
    16621 ctcgacaacc tgtccgcagt cggggacatc gaccacgtct acgtcgtcac caacgccaag
    16681 ttcgccggcc attttcagga gtgggccgaa cgatacaggg acgacagagg caaggccgac
    16741 ttcaccatcg tcaacgacga gtccaccgac gactcgaaca agctcggggc catcggcgac
    16801 ctccacctcg tactcacgcg ggagcgcatc gacgacgaca tcatcgtggt ggcgggcgac
    16861 aacctcttca gtcacagcct ggagaagttc ggcgagttct gccgcgagaa gaaggcgccg
    16921 gtgctggccg tctacgatgt cggcgacctt gaggagatta aaaagtataa cgccatcgat
    16981 gttgacggcg ccgggcggat cactttcttc gaggagaaac cggcgcgccc gaaaagcacg
    17041 ctcacgggaa tcgcgcttta ctactatcca aagtcggcgc tgcccctgat ccgccagtac
    17101 gtcgaggagg ggaacaaccc cgaccagccg ggccggctcg tccagtggat gtacgagcgc
    17161 acgccgttct acacgtggcg cgtgcccgga atctggtacg acgttggctc gaaggagacg
    17221 ctcgaagaag ccaaccggat attctccgac cccgccctca aaggctgaag gccgcggccg
    17281 aggcgtccct cggcttcaag ttttccgccg gtcgttaaga actaaacaca cacgcggaag
    17341 tggcaaggac tatagcaaat gctcgatttg aactttgttc gtgaaaactt tgaacgcgtg
    17401 cgcgcgtcgc tcgaagcgcg cgcgttcccc gtggcggtgc tcgacgactt cgcgcgcctc
    17461 gactccgagc ggcggcgcgt catcgcggag tctgaccagt tgaacgccga gcgcaacgcc
    17521 gccgcgcgcg agatcggcgc gctgatgaag gaggggaaga aggaagaggc cgagggccgg
    17581 cgcgggcggg ccgccgagtt gaaggatcgc atcagtgggc ttgaccgcga gcgcgacgag
    17641 gccgagcgcc gcatgcagga attgctctcg ggcgtcccga acgtccagca cgaaagtgtg
    17701 ccgacgggga cgggcgagga cgacaacgtc gaagcgcgcc ggtggggcaa cagaccggag
    17761 ttagacttcg agccgcagga tcacgtcgat ctcggcgtct ccttgggcat cctcgatctg
    17821 gagcgcgcga cgaagatcgc cgcctcgcgt ttctccatcc tcttcggcgc gggcgcgcgt
    17881 ctggagcgcg ctctcatcca gttcatgctc gacgtacaca cagaggagca cggctacacc
    17941 gagacgctgc cgcccttcat cgtcaacgcg gccgcgctct acggcacgac gcaactcccg
    18001 aagttcgagc aagacctctt caagctcacg gacgagcgcg ggctttatct cagcccgacc
    18061 gccgaggtgc cggtgacgaa catccaccgg gaggagattc tggaagccgc ggagttgccg
    18121 aagaagttcg tcgcctacac gccctgcttc cgctccgagg ctggctccta cgggcgcgac
    18181 acgcgcggca tcatccgcca gcaccagttc gacaaggtcg aactcgtcaa gctcgcgctg
    18241 ccggaagact cctacgcgga gcttgagggg ttgacgcgcg acgccgagac catcttgcag
    18301 agactcggcc tccaccatcg caccgtcacg ctctccacgg gcgacatcgg cttcggctcg
    18361 gccaagacct acgacatcga ggtgtggctg ccctcgcaga acactttccg cgaaatctct
    18421 tcgtgttcca actacgaatc tttccaggcg cgccgcgcgc agatacgctt ccgccgcacg
    18481 cccggtgcca agcccgagta cgtccacacg ctcaacggct cgggcctcgc tgtcggccgc
    18541 acatgggtcg ctattctgga gaactatcaa caacaggacg gctcggtcgt catccccgaa
    18601 gtcctgcggc cgtacatggg cgggatggag aagatagagg ggaggcggca gtaagcagtc
    18661 ggcaatgcgt gttgcgagtt ggagaagagt agtaggaacg cgtgacgagc cgcggagcgg
    18721 cggaagaatg tagcccacgg cgcgagccgt gggtacgagg gccggaaaat ttatagcccc
    18781 ggaggggcga aagaaaatca ggtgcggtca tcacacccgt tgattttctt tcgcccctcc
    18841 ggggcttgtc tttacatctg atgccggttc cacgggacgg tcgcccgtgg ctacattctg
    18901 ccgccgctcc gcggctcgtc gcgcgtcccg ctcgtcttat tcaatcctca actcgaacgg
    18961 catcagggcc agccccttgc cgtgcttcat ctggtcgaag agtcgggcat aagcaaaggc
    19021 gcggcgcagg tcttcgggca gcgagttgat gacggcctgc tcctgccggc gcgcctggag
    19081 ggacgacttg tcttcgtcgc tgtcctcgtc gaagagctgt tggaagaagg tgcgcggggc
    19141 ggggaagacg acgcggcgca cgcccttgtc ggccgggatg ccggccagct ctttggcgat
    19201 ctcgacggcg cggtctaagc cgccgaactc gtccaccagc ccgcgctcct gcgcctgttg
    19261 gcccgtccac acgcgcccct gcgcgatgga gtggacgtac tcgcggtcac gcttgcggcc
    19321 ctcggccacc ttcgggataa attcgtccca gtagaagtgt tcgatgagcg cctcgaagcg
    19381 ggcgcgctca tcgggcgtga acttctccgt ctcgcggaac atgccggcct gcttgccgcg
    19441 cagcacgtac tcgcttgaga tgccgagcca gtcgtagaag ccgctgatga cgggcttgcc
    19501 gccgaagacg ccgatggagc cggtgatggt gccgggctgc gcgacgatgc ggttggctcc
    19561 cgccgcgatg tagtagccgc ccgaggccgc cagatcgctc atcgagatga ccaccggctt
    19621 cttctgcttg gccgcttcaa gcgcgcgcca gatcatgtcc gacgggtaga tgccgccgcc
    19681 ggggctgtcg acgcgcacca cgatggcctt gatccccttg tcgtcgcgcg cgtcgttgat
    19741 ggcgcgcacc acggtgtcgg agccgacctg ttgctcgccg ccgaaggagc cgtcgttgga
    19801 gtggccggag ttgatgacgc ccgaggcgta gatgacggcg atgcgctcgc cctgattgag
    19861 gccgagcgag tcggccgaga cgcgcttgta ctcggagagc gagacggtgt gcagcttctc
    19921 atcgtccttg tagccgccga cgcgcttctt gagttcgttc tcgacctcct cgcgcaccag
    19981 cgcgccgtcg atcaagcccg cctcctgcgc ccggcgcgcg gtgatgggcg cgtcgtcgat
    20041 gagcgcgcgc acgtcctcag gcgacttctt gcggtcgcgc gccacggctt cgacgtagcg
    20101 gttgaaggtg tcgtcgagga tggcgttgat gacctcgcgc tgggcgtcgg acatgtcctt
    20161 gcgcgtgtag gagtcgggcg cgttcttgta cttgccgatc tgatgcacgt ccatctcgac
    20221 gccgagctta tcgagcgagc ctttgaagaa catggcctgc gcggcgaggc cgttgatgaa
    20281 gaggtcgccg gccggcgcga cgtagacgcg gtcgcaggcc gtggcgatgt agagttcctt
    20341 gtcggcgccg tactccatgt agccgtaggc gggcttgccc gaggagcgga agtcggcgat
    20401 ggcgtcgcgt atctcggcgg ccttcgccca gccgacgccg acgaagtcga tgtcgagcag
    20461 cacggcgtcc acgcgcttgt cggccttcgc cttcttgagc tgcgtgagca ggctggtgag
    20521 cgagttggtc tcggcgccga ggaagcgcgc ggcgaacggg tcgtcgttgg cgtaatcggg
    20581 gagcgcgccc gcgaccttca acacgagcac gctgtggtcg cggacgttcg gctcgtgctc
    20641 gcgcagcgag tagaagagca gggccacccc gaggccgacg acgacgacga gcgccatcac
    20701 gattccgagg atgataagaa tcagtttgcg tgtgccagac attgccattg tattttcacc
    20761 ggaaagctaa aaatttagac gcttcgcccc cgtaccgacg gcagcgaccg ctgtagttac
    20821 gaagttgggg agtaaaaggt tttgaagaca gtgagcagtg agcagtgagc agttaaagaa
    20881 aatgcgggtc gcccgtcata actcaactac tgctcactgc tcactgctca cttcttaact
    20941 atccacccgc cgccgacgac ttcctctccg cggtagaaga cggtcgcctg ccccggcgtg
    21001 atggcccgct gaggctcgtc gaagcgaacg cgcgcgcgcc cctccgcgag cggggtgacg
    21061 gtggcgggcg actcctgatg ccggtagcgg acgcgcactt ccgcgcgcac cgagcccgcg
    21121 ggttcgtcga acgccaccca gttgacgccc gccgcggtga actcctgcga cagaagctct
    21181 tcggcgccgc cgatgacaac gcggttcgct tcggcgtcaa cgcggacgac gtagacggga
    21241 agctcgcgcg cgaggccgag gccccgtcgc tgcccgaccg tgtagcgttg gacgcctccg
    21301 tgcgtgccga cgacgcgccc ctcggtgtca acgatctccc ccgcgcccgg tgcgtcgtcg
    21361 cggcctttgg cggcaaggta gcggtcgatg aagccggcgt agtcgccatc gggcacgaag
    21421 caaatctcct gcgactcggc cttctcggcg acgccggaga gattcgcgcc gcgcgcggcc
    21481 tcgcgcgcct ccggtttcga catctcgccg agcgggaaca tggagcgcgc aagttgcgcc
    21541 tgcgtcaact cccagaggaa gtacgtctga tccttgcgcg catcgagtcc gcgcaggagg
    21601 cggtagcggt tcgcgccagc gtcg
//
LOCUS       DASVES010000254         7277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_977826, whole genome shotgun sequence.
ACCESSION   DASVES010000254 DASVES010000000
VERSION     DASVES010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977826"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1213
                     /gene="gcvT"
                     /locus_tag="VF240_10380"
     CDS             95..1213
                     /gene="gcvT"
                     /locus_tag="VF240_10380"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX7175654.1"
                     /translation="MTAPSLKQTPLNAAHRRAGARMVDFGGWDMPVQYPAGTVEEHMG
                     TRTRAGLFDVSHMGEFEVRGPDAIPFINRLCSNDVTKLVDGQAHYSALTTPNGGAVDD
                     LLVYRFGAEHLLLVVNASTIEKDWDWIHAHRGGESVELTNRSADFCQLALQGPDAVPI
                     LQTLTRTPLEEIKYYHFREGQVDGVESIISRTGYTGEDGFEVYAPAERAEQLWERIMD
                     AGRSGTPEGVQPCGLAARNTLRLEAAMALYGHELDEEHTLLEANLGWICKLNKGDFTG
                     RDALQRQKEEGLRRKLVGFEVTERGIARDGQDVLVNGERVGVVTSGSPAPFLKKNIGL
                     AYIPAEAATPGHELQIDVRGRPVGAKIVPTPFYKRERK"
     gene            1378..1773
                     /gene="gcvH"
                     /locus_tag="VF240_10385"
     CDS             1378..1773
                     /gene="gcvH"
                     /locus_tag="VF240_10385"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7175655.1"
                     /translation="MANVPEDLHYSKDHEWIRVEGETGTIGITDYAQHSLGDVVYVEL
                     PKVGDKFEAHESFGSVESVKAVSELFTPVAGEVVEVNETLQDSPESVNSDPYGAAWMM
                     RVRVKDKGAVDALLTAAEYEDFLKTETES"
     gene            1780..3150
                     /gene="gcvPA"
                     /locus_tag="VF240_10390"
     CDS             1780..3150
                     /gene="gcvPA"
                     /locus_tag="VF240_10390"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX7175656.1"
                     /translation="MRYIPNSPEERQEMLSEVGLSQAVDLFDSIPADARLARPLDVPD
                     ALSEIELLARFESLAARNHAAGRPSFLGAGAYSHYSPTIVDALIQRSEFFTAYTPYQP
                     EVSQGTLQAIFEFQTLVCQLTGMDVANASMYDGSTALAEAVLMAERVTRRRRVIVSAA
                     VHPEYLEVARTYVAHAGVELLSAAFDEATGRTNLEDIEGLDETVAAVVVQSPNFFGCV
                     EDVRAAAERAHAVGALLVVAVTESASLGMLRSPGACGADIVIAEGQSFGVPVSCGGPY
                     VGLFATSEKYARQLPGRLVGVAHDKQGRRGFVLTLATREQHIRREKATSNICTNEGLI
                     ALAATIYMTTMGRRGLQEVAEQCAQKAHYAARQIAAVEGYELPYSAPFFNEFVVRAPV
                     AADELLPRLADAHNITGGLALSRYFPGRPNDILVCVTEVNRRSEIDALVAGLRKVQSS
                     KFKVQS"
     gene            3197..4705
                     /gene="gcvPB"
                     /locus_tag="VF240_10395"
     CDS             3197..4705
                     /gene="gcvPB"
                     /locus_tag="VF240_10395"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX7175657.1"
                     /translation="MKKASTHITQNEALIFERSQPGRRGYMLPPLDVDETPADELLPA
                     ELRRDDDLEGVPELSEVDVVRHFTRISTWNFHIDLGMYPLGSCTMKYNSRLNERVARM
                     PGFANLHPLAPEADSQGALEVMYELQRHLSEITGLAGVSLQPAAGAHGELTGVMMIRA
                     FLDKRDGGESARHLMLIPDSAHGTNPASAHLSGFTVKTIRSTPEGLTDLEHLRELCAG
                     GDVAGLMLTNPNTCGIFERNITEICKIVHDAGGLVYMDGANMNALVGVARPGDMGVDV
                     IHLNLHKTFSTPHGGGGPGSGPCCCTAELEPFLPTPLVTRSGDRFRLDFDRPSSIGRV
                     KAFYGNFGMHLRALASILTHGREGLREATEAAVLNARMIAHGLSDIYDKPFDAPPMHE
                     VLFTDKRQSRKGVHTLDIAKRLIDYGFHPMTIYFPLIVQGAMLIEPTESVGRHEIEQF
                     VEAMREIARESVEDPELVLNAPHTTRIGRLDEATAARKPVLRWKPAEKTATA"
     gene            5071..6633
                     /locus_tag="VF240_10400"
     CDS             5071..6633
                     /locus_tag="VF240_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7175658.1"
                     /translation="MKILLYNPDNGVTRNFMPHLWMFLLQALTPPGHEVILVDGNARA
                     MSEDELVSFVRAEGIELVGIGAMTRMVARAYRVADALRAAGVRVVMGGPHVTECPDEA
                     LGRDGGPRHADAIALGEADETWPLMVEDAARGELKEVYQPERDAKGVDRKPSLQPYPD
                     IPWETLALDQFSLVPGFLRPLLGRMGGGWGTFHVVPIETGRGCPYGCEFCTVTGFFGD
                     SIRFRTNESVVGELLRLKERAKRERGQLAVFFIDDNLAINIKRTKQLLRDIIEAGAQI
                     PWVAQISANLLRDEELVDLIAASGGKWVFIGMESIDPANMADVNKNFSKPSEYGAVLE
                     RLARRNVYAITSFIFGMDNDKPGVAERTLKEVNSWPPGLPVFGQLTPFPATPLYERLE
                     KAGRLTRPKHWMDFAPFVMAHTPLHMTIEEAKDEVNQAWSSSYSPERNAEAVEYLRDK
                     HIQYRLSHLVSRLFFRGIYFPQMGKRAWLKVIYQNRKTINGLIREGASTWRAARKRQK
                     QLAAAPPMTDTR"
     gene            6707..>7277
                     /locus_tag="VF240_10405"
     CDS             6707..>7277
                     /locus_tag="VF240_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175659.1"
                     /translation="MRRHFTSAVAFAFTVLLTCACAVAQQKAAPSPTATPQPSPAPSA
                     SPEPLRAVKGQVLTSPAMPAASIEFDKEFKYVGGHSFILYDVARAEQHFFVDADKDGR
                     VKRFYWVQFEGYLPSNTHAYDYKQKVPKSVQIGGLEFIADAYPRNIKANPSPSDSDGA
                     RARSFLESKGYRMAGDDILMQRLVHLVDEA"
BASE COUNT         1325 a         2570 c         2190 g         1192 t
ORIGIN      
        1 cactcgcttg acctgctctc gcgcagcgtg ttaggttcgc ctctgttagc aatacacccg
       61 aacaattcgt gaactcgaac ggcaggagac gagtgtgacc gcgccctccc tcaagcagac
      121 gcccctgaac gccgcgcacc gccgcgcggg ggcgcgcatg gtcgatttcg gcggctggga
      181 catgcccgtc cagtaccccg ccgggactgt cgaggagcac atggggacgc gcacccgcgc
      241 cggtctcttc gacgtgtcgc acatgggcga gttcgaggtg cgcggccccg acgccattcc
      301 cttcatcaat cgcctctgct cgaacgacgt gacgaaactc gttgacgggc aggcgcacta
      361 ctcggcgctc accacaccca acggcggcgc agtcgatgac ctgctcgtct accgcttcgg
      421 agccgagcac ctgttgctcg tcgtcaacgc ctcgaccatc gagaaggatt gggactggat
      481 tcacgcgcac cgcggcggcg agtcggtcga actcacgaac cgcagcgccg acttctgcca
      541 gctcgccctg caaggccccg acgccgtccc gatacttcag acgctcaccc gaacgccgct
      601 cgaagagatc aagtattacc acttccgcga ggggcaggtg gacggcgtcg agtcgatcat
      661 ctcgcgcacc ggctacacgg gcgaggacgg cttcgaggtc tacgcgccgg ccgagcgcgc
      721 cgaacaactc tgggagcgca tcatggacgc cggccgctca ggcacgcccg agggcgttca
      781 gccctgcggc ctcgccgcgc gcaacaccct gcgtctggaa gcggcgatgg ccctctacgg
      841 ccacgagctt gacgaggagc acacgctcct cgaagccaac ctcggctgga tttgtaaatt
      901 gaacaagggc gacttcacgg ggcgcgacgc gctccagcgc cagaaggaag agggcttgcg
      961 ccgcaagctc gtcggcttcg aggtcacgga gcgcggcatc gcgcgcgacg ggcaggacgt
     1021 gctcgttaat ggcgagcgcg tcggcgtcgt cacctccggc agccccgcgc cgttcctcaa
     1081 gaaaaacatc gggctggcct acatccccgc cgaggcggcc acacccggcc acgagctaca
     1141 gatcgacgtg cgcgggcgcc ccgtcggcgc gaagatcgtg ccgacgccct tctacaagcg
     1201 agagcggaaa tgaacagcta atagcgatta gcgattagct gttagctttc agccagaaaa
     1261 ggcgcgggcg gccgtttgag tttattaacg cttgatgttg agcaatagcg aagcctcacg
     1321 gtacggcctc tgccgtgagc taatagctaa tcgctaagag cttctttaag gagaatcatg
     1381 gcaaacgttc ccgaggattt acactacagc aaggatcacg agtggatcag ggtcgagggc
     1441 gagacgggca ccatcggcat caccgactac gcgcagcact ctctgggcga cgtggtctac
     1501 gtcgagctgc cgaaggtcgg cgacaagttc gaggcgcacg agtcgttcgg ctcggtcgag
     1561 tccgtcaagg ccgtctcgga gttgttcacg ccggtggccg gcgaggtcgt cgaggtcaac
     1621 gagacgttgc aggactcgcc cgagtctgtg aactccgacc cttacggcgc ggcgtggatg
     1681 atgcgcgtgc gcgtcaagga caagggcgcg gtggacgcgc tgctcacagc cgccgagtac
     1741 gaagattttc tcaagaccga gacggagagc taaagggccg tgcgctacat ccccaactcg
     1801 cccgaggagc gccaggagat gttgagcgag gtcggtctct cgcaggccgt tgacctcttc
     1861 gactccatcc cggccgacgc gcgcctggcg cgcccgctcg acgtgcccga cgcgctctcc
     1921 gagatcgaac tgctcgcgcg cttcgagagt ctggccgcgc gcaaccacgc cgccgggcgc
     1981 ccgagtttcc tcggcgccgg cgcttactcc cattactccc cgaccatcgt cgatgcgctc
     2041 atccagcgca gcgaattctt caccgcctac acgccctacc agccagaggt ctcgcagggc
     2101 acccttcagg ccatcttcga atttcagacg ctcgtctgcc agttgaccgg catggacgtg
     2161 gccaacgcct cgatgtacga cggctcgacg gcgctggccg aggccgtgct catggccgag
     2221 cgcgtcacgc gccgtcgccg cgtcatcgtc tcggcggccg tccaccccga gtaccttgag
     2281 gtcgcgcgca catacgtcgc gcacgccggc gtcgagttgt tgagcgccgc cttcgacgag
     2341 gcgacgggac gcacgaatct cgaagacatc gaaggactcg acgagaccgt ggcggccgtc
     2401 gtcgtccagt cgcccaactt cttcggctgt gtggaagacg tgcgcgcagc cgccgagcga
     2461 gcgcacgcgg tcggggcgct gctcgtcgtc gccgtcacgg agtcggcctc gctcgggatg
     2521 ctgcggtcgc cgggcgcgtg cggcgcggac atcgtcatcg ccgaggggca gtcgttcggc
     2581 gtgcccgtct cctgcggcgg cccctacgtc ggcctcttcg ccacgagcga gaagtacgcg
     2641 cgccagcttc ccggtagact cgtcggcgtc gcccacgaca agcaggggcg gcgcggcttc
     2701 gtgctcacgc tcgccacgcg cgagcagcac atccgccgcg agaaggcgac ctcgaacatc
     2761 tgcaccaacg agggattaat cgccttggcc gcgaccatct acatgacgac gatgggacgc
     2821 cgcggtcttc aggaagtggc cgagcagtgc gcgcaaaagg cccactacgc cgcgcgccag
     2881 atcgccgccg tcgaaggcta cgagcttccc tactccgcgc cgttcttcaa cgagttcgtc
     2941 gtgcgcgcgc ccgtcgcggc cgacgaactg ctcccacgcc tcgccgacgc gcacaacatc
     3001 accggcggcc tcgcgctctc gcgctacttc cccggccgcc cgaacgacat cctcgtctgc
     3061 gtcaccgagg tcaaccgccg gagcgagatc gacgcgctcg tcgccggtct ccggaaagtt
     3121 caaagttcaa agttcaaagt tcaaagttag aactgcggct tgaacctttg aactttgaac
     3181 tttgaacttt gaacttatga agaaagcctc aacgcacatt acgcagaacg aagctctcat
     3241 cttcgagcgc tcgcagcccg gaaggcgcgg ctacatgctg cccccgctcg acgttgacga
     3301 gacgcccgcc gacgagttgc tgcccgcgga gctgcgccgc gacgacgacc tcgaaggcgt
     3361 gcccgaactc tccgaggtgg acgtggtgcg ccacttcacc cgcatctcca cctggaattt
     3421 ccacatcgac ctcggcatgt acccgctcgg ctcctgcacg atgaagtaca actcgcgcct
     3481 caacgagcgc gtggcgcgca tgcccggctt cgccaacctc cacccgctcg cccccgaggc
     3541 ggactcgcag ggcgcgctcg aagtgatgta cgagcttcag cggcacctct ccgagatcac
     3601 gggccttgcc ggcgtctcgc tccagcccgc cgccggcgcg catggcgagc tgaccggcgt
     3661 gatgatgatt cgcgccttcc tcgacaagcg cgacggcggc gagagcgcgc gccacctgat
     3721 gctcatcccc gactcggctc acggcaccaa cccggcctcc gcgcacctct ccggcttcac
     3781 cgtcaagacc atccgctcga cgcccgaggg attgaccgac ctcgaacacc tgcgcgagct
     3841 gtgcgcgggc ggcgacgtgg ccgggctgat gctcacgaac ccgaacacct gcggcatctt
     3901 cgagcgcaac atcacggaga tttgcaaaat cgttcacgac gccggcggcc tcgtctacat
     3961 ggacggcgcc aacatgaacg ccctcgtcgg cgtcgcccgc cccggcgaca tgggcgtcga
     4021 tgtcatccac ctcaacctcc acaagacctt ctcgacgccg cacggcggcg gcggccccgg
     4081 ctccggcccc tgctgctgca ccgccgagct tgaacccttt ctccccacgc cgctcgtcac
     4141 acgcagtggc gaccgcttcc gtctcgactt cgaccgcccc tcttccatcg gccgcgtcaa
     4201 agccttctac ggcaacttcg gcatgcacct gcgcgccctc gcctccatcc tcacgcacgg
     4261 ccgcgagggt ttgcgcgagg ccaccgaggc ggccgtcttg aatgcgcgca tgatcgccca
     4321 cggtctctcc gacatctacg acaaaccctt cgacgcgccg ccgatgcacg aagtcctctt
     4381 caccgacaag cgacagtcgc gcaaaggcgt ccacacgctc gacatcgcca agcgcctcat
     4441 cgactacggc ttccacccca tgacgatcta cttccctctc atcgttcagg gcgcgatgct
     4501 cattgagccg accgagtcgg tcggccgcca cgagatagag cagttcgtcg aggccatgcg
     4561 cgagatcgcc cgcgagtcgg tcgaagaccc cgagctggtc ttaaacgccc cgcacaccac
     4621 gcgcatcggc cggcttgacg aggccaccgc cgcgcgcaaa cccgtcctgc gctggaagcc
     4681 cgcggagaag accgccacgg cttaacacgt aggacaggca ttcctgcctg tcttgagcgc
     4741 gccagcgcga aagtggattc atcgaatttc aatgttgagt gggcgcggaa agtttattcg
     4801 cgctggcgcg ttcaagacag gcaggaatgc ctgtcctaca tgacagccca cctctcccgc
     4861 gcctcatctt ggctttcatc ggtcaatcag ggtaaactgt gccccgccac acaaagcggc
     4921 gattcgcgct tcgccctcgc gggcggggca ttccaccccc atcatcgacc agagtgcgac
     4981 gcgccctttg gtaaaccggc acaagcggac tgactcgtct ccgtcgctgg gggagtcgaa
     5041 tattagcggc cttctttcgt caacagactt atgaaaatct tactgtacaa ccctgataac
     5101 ggcgtgacgc gcaacttcat gccgcacctc tggatgttcc tcctccaagc actcacgccg
     5161 cccggccacg aggtgattct ggtggacggc aacgcgcgcg ccatgagcga agacgagttg
     5221 gtcagcttcg ttcgcgccga gggcatcgag ttggtgggca tcggcgcgat gacccgcatg
     5281 gtggcgcgtg cctatcgcgt ggccgacgcg ctgcgcgccg cgggagtgcg cgtcgtgatg
     5341 ggcgggccgc acgtcaccga gtgccccgac gaagctctgg gccgcgacgg cgggccgcga
     5401 cacgccgacg ccatcgctct cggcgaggcc gacgagacct ggccgctcat ggtcgaggac
     5461 gccgcgcgcg gtgagttgaa ggaagtctat cagccggagc gcgacgccaa gggcgttgac
     5521 cgcaagccga gcctgcaacc ctaccccgac atcccttggg agactctggc gctcgaccag
     5581 ttcagcctcg tgcccggctt cctgcgcccg ctcctcggac gcatgggcgg cgggtggggg
     5641 acgtttcacg tcgtgcccat cgagacgggg cgcggctgtc cctacggctg cgagttctgc
     5701 accgtcaccg gattcttcgg cgactcgatc cgcttccgca ccaacgagtc ggtcgtcggc
     5761 gagctgctgc gtctcaagga gcgcgccaaa cgcgagcgcg ggcagttggc cgtcttcttc
     5821 atcgacgaca acctcgccat caacatcaag cgcacgaagc aacttctgcg cgacatcatc
     5881 gaggccggcg cgcagattcc gtgggtcgca cagatcagcg ccaacctcct gcgcgacgaa
     5941 gaactcgttg acctcatcgc cgcctcgggc ggcaagtggg tcttcatcgg catggagtca
     6001 atcgacccgg cgaacatggc cgacgtgaac aagaacttca gcaagccgag cgagtacggc
     6061 gccgtgctcg aacgcctcgc ccgccgcaac gtctacgcca tcacgtcctt tatcttcggc
     6121 atggacaatg acaagcccgg cgttgccgag cgcacgctca aagaggtcaa ctcctggccg
     6181 ccggggctgc ccgtcttcgg acagctcacg cccttccccg ccaccccgct ctacgagcgt
     6241 ctggagaagg cgggccgcct cacgcgcccg aagcactgga tggacttcgc ccccttcgtg
     6301 atggcgcaca cgccgctgca catgacgatt gaggaagcga aagacgaggt caatcaggcg
     6361 tggtcttctt catacagccc ggagcgaaac gccgaagcgg tcgagtatct ccgcgacaag
     6421 cacatccagt atcgtctgtc gcatctcgtc tcgcgcctct tcttccgcgg catctacttc
     6481 ccgcagatgg gcaagcgcgc gtggctcaag gtgatttacc agaaccggaa gacgatcaac
     6541 ggcctcatcc gagaaggcgc gtcaacgtgg cgtgccgcgc gcaagcgcca gaagcagctc
     6601 gccgccgcgc cgccgatgac ggacacccgg tgacgcgaag tgcgaccggc tttgtgactc
     6661 acacggcgac tttcggcgag acggggtgag actcggagac gcaacgatga gaagacattt
     6721 cacgtcggcc gtcgccttcg cgtttactgt gctgctcacg tgcgcctgcg ccgtcgccca
     6781 acagaaggcc gcgccgtcgc cgactgccac cccgcagcct tcgcccgcgc cgtcggcttc
     6841 gccggaacca ctccgcgccg tcaaggggca ggtgttgacc tcgcccgcca tgcccgccgc
     6901 cagcatcgag ttcgacaagg agttcaagta cgtcggcggc cacagcttca tcctctacga
     6961 cgtggcgcgc gccgagcagc acttcttcgt cgatgccgac aaggacggcc gcgtcaaacg
     7021 cttctactgg gtgcagtttg aaggctacct gccgagcaac acacacgcct acgactacaa
     7081 gcagaaagtt cccaaatccg tccagatcgg cggtctcgaa ttcatcgccg acgcctaccc
     7141 gcgcaacatc aaggcaaacc cctcaccgtc cgactccgac ggcgcgcgtg ctcgctcatt
     7201 ccttgagagc aagggctacc gcatggcggg cgacgacatc ctcatgcagc gcctcgtgca
     7261 tctcgtggac gaggcca
//
LOCUS       DASVES010000255         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_554757, whole genome shotgun sequence.
ACCESSION   DASVES010000255 DASVES010000000
VERSION     DASVES010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554757"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..1468
                     /locus_tag="VF240_10410"
     CDS             536..1468
                     /locus_tag="VF240_10410"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:722..724,aa:Sec)
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HEX7175660.1"
                     /translation="MFNPLASAAYARADEAAAFVGDGDMVLTVERGNERAAYPVRLMA
                     YHHLVDDMVGGVPLVATYUTLCHTGLVWETTVDGRALHFRLAGINNQNFIMRDEETGS
                     WWQQVSGECILGPLKGRRLKMFEHDELTFAQWRRERPQGRVLRPDPQIEAAGKYAPAD
                     WEQQVARMPVATTLPDGGAGPLEPRTLVAGITTSGGDSKAYPLDALARQSPVIDKVGG
                     TPVLVILNDDGKSVRAFERTVDGRALEFFLKTGVSPLTVVDAETGSEWDFTGTATAGP
                     LAGRQLRKIHALKDYWFDWKTYNPETAVYLLGNR"
     gene            1814..>3669
                     /locus_tag="VF240_10415"
     CDS             1814..>3669
                     /locus_tag="VF240_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7175661.1"
                     /translation="MHVTRVELDNVKSYERREFVFERGTTAIVGENGAGKTTILEAIA
                     WTLFDVLEYPKDDFLRRGAKKGSVRVTFQSDLDERQYTVYRDTGQGYHVYDPELKVKL
                     AEKKADVRSFLNLHLGVEPGTDLKSLFRSAIGVPQGFFTTDFLRSPNERKAAFDRLLK
                     VEEYRESAERLRSTINLIGERRAEARGRVSGFDAQLARYEEITDEHKTALARAGELSD
                     ALDALQSEAETRASTVARFDEAESAVAETRAAADRLEVESESAARRLEDLRAALDAAR
                     RASDRQRATEADYRAHLEAAEHLRALETERTERDRLLAEAAGVASLAAAGESDVRRLE
                     ESLERAAGARVSLAAIEKDVARQEELERERERLRDLLAHARAAADRLERLDRELMVLR
                     RQHAETRERVRAAEGAAGAQEKVERLESERLGVDNRLNEVNRALTESRLLGGQRRESA
                     REVERLTKAVAALEREAREQEQQAAVAGRTPELEASERELAREAAGLRASIEHDERVR
                     QQTRGGVCPILGEQCTSFGEGRDFDAYFGDQLKKNRAQLATAEKELGRVEREVVTARE
                     AERQLARFETSRRRLSEERALLSEREAALSRLDAQLAALPPATEQLKDQLQT"
BASE COUNT          626 a         1249 c         1238 g          556 t
ORIGIN      
        1 cttcggcctt gccgaaagcc tcaaggcgca ggggcgcgcg cacgaatcgg aactcgtccg
       61 ccgcgagttc gagcgcgcct ggaagggcgc cgacacgaag ctgcgcgtgg aagaccttta
      121 gatagcggac tactcacaac agtaccgcga gcgatagcga gcgggtgtgc gtactctggc
      181 aagtcctgac ctacagtcgc acccgctcgc taccgctcgc ggtactgtgt tcgaggtaac
      241 gatgaaacgc tggctcctcc tgctcctcct gtcggtcgcg gcgctcgcgc tcgtcgctgt
      301 gcccgtgtgg ctcattcaac cctttagccc gcagacgccg cgcgcgcttg aggtcggcta
      361 cgcgctgcgc cgttggtcgc cgctcgtcac gctggcgttg gcggccggcg ggctagcact
      421 cgtcttccgt ctatggcgcg actcgcgccg atggtgggcg aagggtttca tgctcctgct
      481 gctcgcgccc gtcttcgcgt cggcgtggtt cgcgcggcag aaccatttcg agtggatgtt
      541 caacccgctc gccagcgcgg cttacgcgcg ggccgacgag gccgccgcgt tcgtcggcga
      601 cggcgacatg gttctgacgg tcgagcgtgg caatgagcgg gcggcctacc ccgtccgtct
      661 gatggcttat catcatctcg tcgatgacat ggtcgggggc gtgccccttg tcgccaccta
      721 ttgaacgctc tgtcacaccg gtctggtgtg ggagacgact gtagacgggc gcgcgcttca
      781 cttccggctc gccggcatca acaaccagaa cttcatcatg cgcgacgagg agaccggctc
      841 gtggtggcaa caggtctcgg gcgagtgcat cctcggcccg ctcaaaggcc gccgcctgaa
      901 gatgttcgag cacgacgagc tgaccttcgc gcagtggcgg cgcgagcgtc cacagggccg
      961 cgtgctgcgt cccgacccac agatcgaagc ggccggcaag tacgcgcccg ccgactggga
     1021 gcagcaggtg gcgcgcatgc ccgtcgcgac gacgctgccc gacggcggag ccggcccgct
     1081 tgagccgcgc acgctcgtcg ccggcatcac cacgtcgggc ggcgactcga aggcttaccc
     1141 gctcgacgcg ctcgcgcgtc aaagccccgt cattgataaa gtgggaggca cgcccgtcct
     1201 cgtcatcctc aacgatgacg gcaagtcggt gcgcgccttc gagcgcacgg tggacggtcg
     1261 cgcgcttgag tttttcctga agacgggagt ctcgccgttg acggtcgtcg atgcggagac
     1321 cggcagcgag tgggacttca cgggcacggc caccgcgggg ccgctcgccg gccgccagct
     1381 caggaagatt cacgccctca aggactactg gttcgactgg aagacttaca accctgagac
     1441 ggccgtctat ctgctcggca atcgatgatt ctgtggggac agggcaagtc ggcggccctg
     1501 actggtgggc aaatttcaat gttagagcgg tgttcagttg agtgaaggca gaatagattc
     1561 accgcggagt cgcggaggcc gcggaggggg cgcggagggt tgaatctctc tgcgtgaact
     1621 gctgcgtcct ccgcgactcc gcggtgaact tcctatcaac ttaaaactgg tattggtcaa
     1681 cgctctgaca ctcctcacgc gctagcaaac cggcgcacga taccttagac tccttgcgcg
     1741 aatcgacacg cttcccgcgc gcccgcagct tctccgaaga gtcgctgcct gcgccgcatc
     1801 aattccgaaa agcatgcacg tcacacgcgt cgagctggat aacgtcaagt cttacgagcg
     1861 ccgcgagttc gtcttcgagc gcggcacgac cgccatcgtc ggcgagaacg gcgctggcaa
     1921 gacgaccatc ctcgaagcca tcgcctggac gctcttcgac gtgctcgaat accccaaaga
     1981 cgatttcctc cgccgcggcg ccaagaaagg ctccgtgcgc gtcaccttcc agagcgacct
     2041 cgacgagcgg caatacaccg tctaccgcga cacgggtcag ggctaccatg tctacgaccc
     2101 cgagctgaaa gtgaagctcg ccgagaagaa agccgacgtg cgctcgttcc tgaacctgca
     2161 cctcggcgtt gaaccgggca ccgatctcaa gtcgctcttt cgctcggcca tcggcgtccc
     2221 gcagggcttc ttcaccactg atttcctgcg ctcgcccaac gaacgcaagg ccgccttcga
     2281 ccgcctgctc aaagtcgaag agtatcgcga gtcggccgag cgcctgcgct cgaccatcaa
     2341 cctcatcggc gagcgccggg ccgaggcccg cgggcgtgtc agcggcttcg acgcgcagct
     2401 cgcgcgctac gaagaaataa ccgacgaaca caagacggcg cttgcgcgcg ccggcgaact
     2461 gtccgacgca ctcgacgcct tgcaaagcga ggccgagacg cgcgcctcaa cggtcgcgcg
     2521 cttcgacgaa gccgagagcg ctgtggccga gacgcgcgcg gcggccgacc gcctcgaagt
     2581 cgaatcggag tcggccgcgc gccgcctcga agacctgcgc gcggcgctcg acgcggcgcg
     2641 gcgcgcgagc gaccggcagc gcgccaccga agccgactac cgcgcgcacc tcgaagcggc
     2701 cgagcacttg cgcgcgctgg agaccgaacg caccgagcgc gaccgactgt tggccgaagc
     2761 cgccggcgtc gcgtcgctcg cggcggcggg cgagtcggac gtgcgccgtc tggaagagtc
     2821 tctggagcga gcggcgggcg cgcgcgtgtc gctcgccgcc atcgagaaag acgtggcccg
     2881 tcaggaggaa ttggagcgcg agcgcgagcg cctgcgcgac ctgttagcgc acgcgcgcgc
     2941 cgccgccgac cgtctcgaac gactcgaccg cgagctgatg gtgctgcgtc gccagcacgc
     3001 ggagacgcgc gagcgcgtgc gtgccgccga gggcgcggcc ggggcgcagg agaaggtgga
     3061 acgccttgag agcgagcgtc tcggcgtaga caaccgcctc aacgaagtga accgggcgct
     3121 gaccgagtcg cggctcctcg gcgggcagcg gcgcgagtcg gcgcgcgagg tcgagcgttt
     3181 gacgaaggcg gtcgccgctc ttgagcgcga ggcgcgcgag caggagcaac aggcggccgt
     3241 cgcgggccgc acccccgaac tcgaagcgag cgagcgagag ctggcgcgcg aggccgcggg
     3301 cctgcgcgcc tccatcgagc acgacgagcg cgtccgacag cagacgcgcg gcggcgtctg
     3361 ccccatcctc ggcgagcagt gtacgagctt cggcgagggg cgcgatttcg acgcctactt
     3421 cggcgaccag ttgaagaaga atcgcgcgca gctcgccacg gccgagaaag agttgggccg
     3481 cgtcgagcgc gaggtggtga cggcgcgcga ggccgagcgc caactcgcgc gcttcgagac
     3541 ctcgcgccgc cgcctcagtg aagagcgcgc gctcctctcc gagcgcgagg cggcgctctc
     3601 gcgccttgac gcgcagcttg ccgccctccc gccggccacc gagcagttga aagaccagtt
     3661 gcagacaga
//
LOCUS       DASVES010000256         6019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_598321, whole genome shotgun sequence.
ACCESSION   DASVES010000256 DASVES010000000
VERSION     DASVES010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598321"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            598..5412
                     /locus_tag="VF240_10420"
     CDS             598..5412
                     /locus_tag="VF240_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX7175662.1"
                     /translation="MKSQQILRVTLLVLLVGTLLLSGVFVGSAQIGASAPAPQTGLTT
                     GQTVGQTTGQITGLAKPPRPQGIDVRGPNGVPKGLALRQPTEVQLEAIEALQTSLGGA
                     QFNIQYNALTATPRHIYSQGAYLSAPSSADPETIARGFISQWRAVFRFNQRDVDSLRL
                     KSRATLADMGTTVLLFEQTRDALPVYKGEVLVNVNRAGQIMSVGGESFPQMAVTNVFA
                     LPEAQAVTAAAAALGINGFVPVAKGTRKVLTTFGDLPHEFTTGTRFARGVFTDDIVVT
                     KTIFPLGAEGRPAYTFTLTTPQYEGIMWLHVVDAQTGAILRRNSLTAFQQGGGPSNSR
                     RSTFRPDIQDAAEATPTTNASAKVFDGQPTALSGPAGFGRTPRGNPPTYAPEATTTAP
                     GRAFRRSWVFARNAAPFADPATSLFSQIYNTPFGQVVRGMPNAQHPTAGSPFGWFYLP
                     TANSGAEITTDNANRATTRAYGYTMDLESRNRNEDSNSPTGDGDQPYSATVTPLGAPV
                     ITQDGRNLTSVIQSNYTEGNNVLVADDHANDNETTHGVKGYSAGRHFTAAYFDFTNSY
                     EYGGVDAGGTPFFPATTNADVYPGTTTLFYYNNLIHDYLYEIGFTEATWNFQQDNFGK
                     GGAGRDGVSAQVQDGSGTDNANFGTPNDGSTPRMQMFLFTEGGARRSDGDFDFDIVAH
                     ELYHGVSNRSVGKGTSDCLGNPIVGESGGMGEGWGDFVASSMTDDDAEGEYATGNFIS
                     GIRKLPYTNHRYSYATANGAPRRRDQQPPGTGEVIFLPFEVHEVGELWAPLLWDMREL
                     MVVKQNSSAIFFDGTRRLGSGTSFFIGPRQVQSVDTQHPINYRAAFNTASVTTIIPAQ
                     HAVRPGAIAAEIQSLGHRNGPVASAVRAGARLADTITLRGMQLSPCNPSFVEMRDSML
                     AADRELTGGENQSIMWRAFASHGVGVLAASTAGAADDGGSQAAPAVVEDFSVPAGVTE
                     CETLGPLAPPSFTLSNPSNNTARVTITPNIAAVTYIISRGDSANGPFVKIAEITSEQV
                     TYDDAGLPGQRTYFYQVRASRNPQCVSTANTQSIFVAGDAVTPSPLFGGLDRVEDPRD
                     GSRLILSWGPAVSLNPAANIVYDIYRVTHVDHTNDVGPPADADAPGDPTFTPDASNLV
                     ATVTGTSYVDTGLDLAQVYYYIVQARDTTNGKIDSGNTGNRVTRFNAPTISQFAANPP
                     FALETFETAAASSRFTPPLTEATTNPNQNSPTFQRITVANLGNPTAGKMYAPDFSPGD
                     EQGLPDPAGTHHGAQSDFFTQIGPFNGAGNPALTATSIMEFDNTINSEARFDGGNIEV
                     KVGAPFVQGDATPFPDNVVTFDLGNYIVEGGYNGLLDGSLPACPPAPAPCKGSGLQGR
                     RAYTGLKGLHHVRAMLHNFAPGGLHNPQGLPVYIRFRMSSDVASAAGLDSGWFVDNLV
                     INNLACRVNVAHSETGATAEASSTQTERNYTPAGAINGDRKGQDWENGDGWNDNTRDV
                     WPDWLVVNFNGSNSISEVRVYTLQNNYRSAVEPTPDMLADFYGITDFDIEYWNGAAWV
                     TVPGGAIRGNDKVMRTVVFPEVTTTKIRINVLGGRVYFSRIVEVEAFGCPAQ"
     gene            complement(5633..>6019)
                     /locus_tag="VF240_10425"
     CDS             complement(5633..>6019)
                     /locus_tag="VF240_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175663.1"
                     /translation="EAKDVGPRQVELRVGEETTLPDSKIKIRFLSVDNDSRCPQGVNC
                     IWAGSVGVSLLLSAPDAEDERVKLNTAVEPQSVSYKGHSLRIESVSPPRVEGKKITPE
                     DYRITLAVCAEKSGAHKTGDVSDPAS"
BASE COUNT         1106 a         2233 c         1746 g          934 t
ORIGIN      
        1 gcgcttgagc aaagcttgaa cgtttcagtt aacgagagaa ctttagccgg ggcaggttga
       61 ggaatccttg aggcgggcga aaccatttaa aaacggcctt ctcatcgtgt taggccgaag
      121 gtcggatgag gagtttggcc agtgtaaaac aggctggggc ttgggaggag catgcctctt
      181 caactgagtg ggtggagaga ctgactcacc gcagaggcgc cgcaggacgc agagttcacg
      241 cagaggcatt gaatcaactc aagcctccgc gccccctctc gcgtcctctg cgcctctgcg
      301 gtgaatcaat ctctccacac actcaactga aagggcatgc tcctcccaag ccccagcctg
      361 ttttacaccg gccaaactcc catccgacct tcggcccaaa ccgttaaaga aagccgtttt
      421 taaaaggttt cgcccgcctc aaggattcct caacctcccg gtactaaatt ctccccgtta
      481 actgtaacgt tcaagctttg ctcaagcgcc ggggaatatt ctcttttcac ggtcagtcgg
      541 caattaaaaa aagaaaaggc gcggcgccgc ccgcccattc ggtcaaggag aagatcaatg
      601 aagtcccaac agatacttcg agtcacgctg ttagtcctgc tcgtggggac gctcctgctc
      661 tcaggcgtct tcgtcggctc cgcccagatc ggcgcgtcgg ctcccgcgcc ccagaccggc
      721 ctgacgaccg gacagacggt cggccagacg acggggcaga ttaccggcct cgccaagcct
      781 ccccgcccgc agggcatcga cgtgcgcggg cccaacggcg tgccgaaggg gctggcactc
      841 cgccagccga ccgaggtgca gctcgaagcc atcgaggcgc ttcagacgtc gctcggcggc
      901 gcgcaattca acatccagta caacgctctg acggccacgc cgcgccacat ctacagtcag
      961 ggcgcatacc tctcggcccc gtcgagcgcc gaccctgaaa caatcgcccg cggcttcatc
     1021 agccagtggc gcgccgtctt ccgcttcaac cagagggacg ttgactcgct ccgcttgaag
     1081 agccgcgcga cgctcgccga catggggacg accgtgctcc tgttcgagca gacgcgcgac
     1141 gctctgccgg tctacaaggg cgaagtgctg gtgaacgtca accgcgccgg ccagatcatg
     1201 tcggtcggcg gcgagagctt cccgcagatg gccgtgacga acgtcttcgc actccccgag
     1261 gcgcaggccg tgacggccgc cgccgccgcc ctcggcatca acggcttcgt gccggtggcg
     1321 aaggggacga ggaaggttct gacgaccttc ggcgacctgc cccacgagtt caccactgga
     1381 acgcgcttcg cccgcggcgt cttcaccgac gacatcgtcg tcaccaaaac gatcttcccg
     1441 ctcggcgccg aggggcgtcc ggcctacacc ttcacgctga ccacgccgca gtacgagggc
     1501 atcatgtggc tccacgtcgt ggacgcgcag acgggcgcca tcctgcggcg caacagtcta
     1561 acggcgttcc agcagggcgg cggcccgagc aacagccggc gctcgacctt ccgccccgac
     1621 attcaggacg ccgccgaggc cacgcccacc accaacgctt cagccaaggt cttcgacggc
     1681 cagccgacgg ccctctccgg ccccgcgggc ttcggccgca cgccccgcgg caaccccccg
     1741 acctacgccc ccgaagccac gaccactgcc ccgggccgcg ccttccgccg cagttgggtc
     1801 ttcgcgcgca acgcggcccc cttcgccgac ccggccacgt cgctcttctc gcaaatctac
     1861 aacacgccct tcggccaggt cgtgcgcgga atgcccaacg cgcagcaccc gaccgccggc
     1921 tcgcccttcg gctggttcta cctgccgacc gcaaacagcg gcgccgagat caccactgac
     1981 aacgccaacc gcgccaccac ccgcgcctac ggctacacga tggaccttga gtcgcggaac
     2041 cgtaacgagg actcgaactc gcccacgggc gacggcgacc agccctactc ggccaccgtc
     2101 accccgctcg gcgcgcccgt catcacgcag gacggccgca acctgacctc ggtcatccag
     2161 tcgaattaca ccgaaggcaa caacgtgctc gtcgccgatg accacgccaa cgacaacgag
     2221 acgacacacg gcgtcaaggg ctacagcgcg ggccggcact tcaccgccgc ctacttcgac
     2281 ttcaccaatt cttacgagta cggcggtgtg gacgcgggcg gcacgccctt cttcccggcc
     2341 acgaccaacg cggacgtgta ccccggcacg acgacgctct tctactacaa caacctgatc
     2401 cacgattacc tctacgagat cggcttcacc gaggcgacgt ggaacttcca gcaggacaac
     2461 ttcggcaagg gcggcgcggg gcgcgacggc gtctcggcgc aggtgcagga cggctccggc
     2521 accgacaacg ccaacttcgg cacgcctaac gacggctcga ccccgcgcat gcagatgttc
     2581 ctcttcaccg agggcggcgc ccgccgctcg gacggcgact tcgacttcga catcgtcgcc
     2641 cacgagcttt accacggcgt ctcgaaccgc tcggtcggca agggcacgtc cgactgtctg
     2701 ggtaacccca tcgtcggcga gtcgggcggc atgggcgaag gctggggcga cttcgtcgcc
     2761 tcctcgatga ccgacgacga cgccgagggc gagtacgcca ccggcaactt catctccggc
     2821 atccgcaagc tgccctacac caatcaccgc tactcatatg cgaccgccaa cggcgcgccc
     2881 cgtcgccgcg accagcagcc gccgggcacg ggcgaggtaa tctttctccc gttcgaggtt
     2941 cacgaggtcg gcgaactctg ggcgccgctt ctgtgggaca tgcgcgagct gatggtcgtc
     3001 aagcagaact cttcggccat cttcttcgac ggcacgcgcc gcctcggctc cggcacgtcg
     3061 ttcttcatcg gcccgcgtca ggtgcagtcg gttgacacgc agcacccgat caactatcgc
     3121 gccgccttca acacggcgag cgtcaccacc atcatcccgg cacagcacgc cgtgcggccc
     3181 ggcgccatcg ccgcggagat tcagtcgctc ggccaccgca acggccccgt cgccagcgcg
     3241 gtacgcgccg gcgcccgcct cgccgacacc atcacgctgc gcggcatgca gctctcgccc
     3301 tgcaacccga gcttcgtcga gatgcgcgac tcgatgctcg ccgccgaccg tgagctgacg
     3361 ggcggagaga accagtcgat tatgtggcgg gcgttcgcca gccacggcgt cggcgtcctg
     3421 gccgcctcca ccgcgggcgc ggccgacgac ggcggcagcc aagccgcgcc cgcagtcgtc
     3481 gaagacttca gcgtccccgc cggcgtcacc gagtgcgaga cgctggggcc gctcgccccg
     3541 ccctcgttca ccctctcgaa cccgagcaac aacaccgccc gcgtgacgat tacgcctaac
     3601 atcgcggccg tcacttacat catctcgcgc ggcgatagcg ctaacggccc cttcgtcaag
     3661 atcgccgaaa tcacctccga acaggttacc tacgacgacg cgggcctgcc cggtcagcgc
     3721 acatattttt accaggtgcg ggccagccgc aacccgcagt gcgtcagcac ggccaacact
     3781 cagagcatct tcgtcgccgg cgacgccgtc acgccctcgc cgctcttcgg cggcctcgac
     3841 cgggtggaag acccgcgcga cggctcgcgt ctcatcctga gctgggggcc ggccgtctcg
     3901 ctcaacccgg cggccaacat cgtctacgac atctaccgtg tgacgcacgt cgatcacacc
     3961 aacgacgtcg ggcctccggc cgacgccgac gcgccgggcg acccgacctt cacgcccgac
     4021 gcctcgaacc tcgtcgccac cgtcaccggc acgtcctacg tggacacggg cctcgacctt
     4081 gcacaggtct actactacat cgtgcaggcg cgcgacacca ccaacggtaa gatcgactcc
     4141 ggcaacaccg gcaaccgcgt gacgcgattc aacgcgccga ccatctcgca gttcgcggcg
     4201 aacccgccgt tcgcgctcga aaccttcgag accgccgcgg ccagcagccg cttcacgccg
     4261 ccgctcaccg aagcgacgac gaacccgaat cagaactcgc cgaccttcca gcgaatcacg
     4321 gtcgccaacc tcggcaaccc gaccgccggc aagatgtacg cgcccgactt cagccccggc
     4381 gacgagcagg gcttgcccga cccggccggc acgcaccacg gcgcacagag cgacttcttc
     4441 acccagatcg gcccattcaa cggggccggc aacccggcgc tgaccgcgac ctccatcatg
     4501 gagttcgaca acacgatcaa ctccgaggcc cgcttcgacg gcggcaacat cgaggtcaag
     4561 gtcggcgcgc cgttcgttca gggcgacgcc acgccgttcc ccgacaatgt cgtcaccttc
     4621 gacctcggca actacatcgt cgagggcggc tacaacggcc tgctcgacgg ctcactgccg
     4681 gcttgcccgc ccgcgccggc tccgtgcaag gggtcgggct tgcagggtcg tcgcgcctac
     4741 acgggcctca agggtctgca ccacgtccgc gccatgctcc acaacttcgc gccgggcggg
     4801 ctgcacaacc cacagggcct gccggtctac atccgcttcc gcatgtcgtc cgacgtggcc
     4861 tcggccgccg gcctcgattc cggctggttc gtcgataacc tcgtcatcaa caacctcgcc
     4921 tgccgcgtca acgtcgctca ctcggagacg ggcgcgacgg ccgaagcgtc gtcaacccag
     4981 accgaacgca actacacgcc cgccggggcc atcaacggcg accgcaaggg ccaggactgg
     5041 gagaacggcg acggctggaa cgacaacacg cgcgacgtgt ggcccgactg gctcgtcgtc
     5101 aacttcaacg ggtcgaactc gatcagcgag gtgcgcgtct acaccttgca gaacaactac
     5161 cgcagcgccg tggagccgac gcccgacatg ttggccgact tctacggcat cacggacttc
     5221 gacatcgaat actggaacgg ggcggcgtgg gtcaccgtgc cgggcggggc catccgcggc
     5281 aacgacaagg tgatgcggac ggtcgtcttc ccggaggtga cgacgacgaa gatcagaatc
     5341 aacgtcctcg gcgggcgcgt ctacttcagc cgcatcgttg aagtcgaagc cttcggctgt
     5401 ccggcccagt aacaacctga cgccgaaaac aacacagccc cgcggcgctc gcacgcgccg
     5461 cggggcttct ttcttaatgt aggacaggca ttcctgcctg tcttgagcgc gccggcgcga
     5521 ataaactttt cgcgctcacc cgaagttgag gctcaacgaa tcttcctttc gcgctcacgc
     5581 gctcaagaca ggcaggaatg cctgtcctac acgaccgccc cgttgaaatt tactatgagg
     5641 cggggtcgga cacgtcgccg gtcttgtgag cgccggattt ttccgcgcac accgcgagcg
     5701 tgatgcgata gtcttcgggc gtgattttct tcccctccac tctgggaggg ctgaccgact
     5761 cgattctgag gctatggccc ttgtaggaga cggactgcgg ctcgaccgcc gtgttgagct
     5821 tcacgcgctc gtcttcggcg tccggcgcgg agagcagcag gctgacgccc acgctgccgg
     5881 cccagatgca gttgacgccc tgcgggcacc gcgagtcgtt atccacggag agaaacctga
     5941 tcttgatttt cgagtccggc agcgtcgtct cctcccccac tcgcagctcg acctgccgcg
     6001 gccccacatc cttcgcctc
//
LOCUS       DASVES010000257         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1557402, whole genome shotgun sequence.
ACCESSION   DASVES010000257 DASVES010000000
VERSION     DASVES010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557402"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF240_10430"
     CDS             complement(<1..278)
                     /locus_tag="VF240_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175664.1"
                     /translation="MKRLFPLCVLVALVSWCALYATAYGTPSPPAGQPAPQSVTLDLS
                     SFGAVGDGVADDGPAIKRALDALADAGGGTLTVPLGRFAILTPVIKDFR"
     gene            complement(746..>2479)
                     /locus_tag="VF240_10435"
     CDS             complement(746..>2479)
                     /locus_tag="VF240_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX7175665.1"
                     /translation="SQPMGGVSTGTALVSATRRTVGVVDPKAPKVFEDVTAQTGLAAF
                     RHRSGSAQKDYIVETVSGGVALFDYDADGRPDIYLLNGSTIEAARGKEKPPRAALFRN
                     LGNWKFEDVTERAGVANERWGMGVAVGDYDNDGDSDLYVGNFGVSRLYRNNGDGTFTD
                     VAEKLGVARKGWSTGASFGDYDADGRLDLFVPGYVELDLNNLPPNPSEAAKPGQIAQN
                     FCQFRGVPVMCGPRGLKGETDTLYRQKSDGGFEDVSAKTGVNDEPRYYGFSSVWVHAN
                     DDHLIDLLVVNDSTPKQLYINLGDGKFEETGYPSGVALNENGREQAGMGLAVGDYDND
                     GRLDFYITNFSDDSNTLYHNDGEGNFTDVTFQAGHGEVTIPFLGWGTSFLDFDNDGWR
                     DLVVANGHVYPAVDKNQWGTSYAQQLLLFRNLSTGRFERVGAAPGTALAQAWLSRGLA
                     VGDLDADGRQDVVLNNMDGAPTVLRNVTQTPNHWLRLQLVGDPARKSPRDATGAAVYL
                     TAGKTTQRDDRVSGAGYSSQNDPCPFFGLGAAAKVDKLEVVWPGGTREVFDVPGVDRT
                     LTLVEGKGAKP"
BASE COUNT          416 a          724 c          905 g          434 t
ORIGIN      
        1 cggaagtctt taatgaccgg cgtgaggatg gcgaaacggc cgaggggcac ggtgagcgtc
       61 ccgccgccgg cgtcggcgag cgcgtccaaa gccctcttga tcgcggggcc gtcgtcggcc
      121 acgccgtcgc cgacggcgcc gaatgagctg aggtcaaggg tgacggactg cggggcgggt
      181 tgcccggcgg gcggggaagg cgtgccatag gccgtcgcat agagggcaca ccacgaaacg
      241 agcgccacga gcacgcagag ggggaacagt cgtttcaatt gattaatcct cggtcgcgcc
      301 tgcgggccgc acgggtgaac aatgcgccga tccgggttgg gagtactgcg aggagggtgg
      361 gcgtgtactt ggggaggggc tgccgctccg cttccgcgtc ttaagacgcg ctcggccaaa
      421 ggttggacga ataaagatgt gaagcggtaa ggctccggtc gtgccgcact tccggaaaaa
      481 ggacggggct actgacgaat catcctcgaa gacgcgccgg aagcaaaaag tctttgccac
      541 gtgtgacgcg caatcttcca atacttttta taaagagtac gcggggagtt gtcaacaggg
      601 aggtcaaaaa atatcagttt gcattttgtg cggggctcat ccggggcggg gaaagggaac
      661 acggattaaa cggataagac ggatgagcgc cgattcgatc cgtgctcatc cgtcttatcc
      721 gtgttccacc tccggcgcgc gcggtctacg gcttcgcgcc cttgccctcg acgagtgtga
      781 gtgtgcggtc aacgcccggc acgtcgaaga cttcgcgtgt cccaccgggc cagaccactt
      841 cgagcttatc gactttggcc gccgcgccga ggccgaagaa ggggcagggg tcgttctgcg
      901 aagagtagcc ggcgccgctc acgcggtcgt cgcgctgcgt ggtcttgccc gcggtgaggt
      961 agacggcggc gcccgtggcg tcgcgcggac tcttgcgcgc cgggtcgccg acgagctgga
     1021 ggcgcagcca gtggttcggc gtctgggtga cgttacgcag caccgtgggc gcgccgtcca
     1081 tgttgttgag cacgacatcc tgacggccgt cggcgtcgag gtcgccgacg gcgaggccgc
     1141 gcgagagcca cgcctgcgcg agggccgtgc cgggcgcggc gccgacgcgc tcgaagcggc
     1201 ccgtggagag gttgcggaag aggagtaact gctgggcgta ggaggtgccc cactggtttt
     1261 tgtccaccgc ggggtagacg tggccgttgg cgacgacgag gtcgcgccag ccgtcgttgt
     1321 cgaagtcgag gaagctggtg ccccagccga ggaaggggat agtcacctcg ccgtggccgg
     1381 cttgaaaagt aacatcggtg aagttgccct cgccgtcgtt gtgatagagg gtgttggagt
     1441 cgtcggagaa gttagtgatg tagaagtcga ggcggccgtc gttgtcgtag tcgccgacgg
     1501 cgaggcccat gccggcctgc tcgcgcccgt tttcgtttaa ggcgacgccc gaagggtagc
     1561 ccgtctcctc gaacttgccg tcgccgaggt tgatgtagag ctgcttcggg gtggaatcgt
     1621 tgacgacgag aaggtcgatg agatggtcgt cgttggcgtg tacccagacg gaggagaagc
     1681 cgtaatagcg cggctcgtcg ttgacgcccg tcttcgccga cacgtcctcg aagccgccgt
     1741 ccgacttctg tcggtagagc gtgtcggtct cgcccttgag gccgcgcggg ccgcacatga
     1801 cggggacgcc gcggaactgg cagaagttct gcgcgatctg gccgggcttg gcggcctccg
     1861 aggggttggg cggcaaattg ttgagatcaa gctcgacgta gccggggacg aagaggtcga
     1921 ggcggccgtc ggcgtcgtag tcgccgaagc tggcgccggt tgaccagccc ttgcgggcga
     1981 cgccaagttt ctcggccacg tccgtgaaag tgccgtcgcc gttgttgcgg tagagacgag
     2041 agacgccgaa gttgccgacg tagaggtctg agtcgccgtc gttgtcgtag tcgccgacgg
     2101 cgacgcccat gccccaacgc tcgttagcga cgccggcccg ttccgtcacg tcctcgaact
     2161 tccagttgcc gaggttgcgg aagagagcgg cgcgcggcgg cttctctttg ccgcgcgcgg
     2221 cctcgatggt cgagccgttg agaagataga tgtcgggccg cccgtccgcg tcgtaatcga
     2281 agagggcgac gccgcccgag acggtttcga cgatgtagtc cttctgcgcg cttcccgaac
     2341 ggtggcggaa ggcggcaagg cccgtctgcg ccgtcacgtc ctcgaagacc ttcggagcct
     2401 tcgggtcaac gacgccgacc gtgcgccgcg tggccgagac gagcgccgtg cccgtcgaga
     2461 cgccgcccat cggctgcga
//
LOCUS       DASVES010000258         7604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1407607, whole genome shotgun sequence.
ACCESSION   DASVES010000258 DASVES010000000
VERSION     DASVES010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407607"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VF240_10440"
     CDS             <1..1282
                     /locus_tag="VF240_10440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7175666.1"
                     /translation="LLDIDLPDGSGLDALREIKNRQPEAVVIMITANVLIQNTISALR
                     GGAYDFVGKPLNLEELRVTIRNGIEASRLRREVTQVRRERARQFSFDQIIGDSPAVKR
                     MLSLARKVAESEASSVLLQGESGTGKDLVAKAIHYGSRRADAPFVAINCAAIPSTLIE
                     SELFGYEKGAFTDAKARKEGLFEQAEGGTLFLDEIGELELGLQAKLLRVLEEGSFRRV
                     GGLKDIPLDVRVVAASNRDLKTEGEAGRFRVDLYYRLSVIQIDIPPLRGRGDDTLLLA
                     EHYVKTFGERLRLKNKIRGLSPEVREIFLQYAWPGNVRELRNVIERALILEDGDLVTM
                     EYLPRDLTGRTEPVASPGAEGGGAAHLFRLPPDGVPLEEVENSLVRQAVERSNGNQTR
                     AAELLRISRDQLRYRLKKLESPAPHDKTATEAAS"
     gene            1299..3254
                     /locus_tag="VF240_10445"
     CDS             1299..3254
                     /locus_tag="VF240_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7175667.1"
                     /translation="MSESTLHETTQTGERPFDTEEALRRSLKELADLKFALDESAIVA
                     ITDQRGVINYINDKFCQISKYSREELLGQDHRIINSAYHPEEFIRNLWTTIASGQVWR
                     GEIRNRAKDGSIYWVDTTIVPFLNPEGKPYQYVAIRYDITERKRAEERIGEQAALLDK
                     AQDAIVVRDAGDNKILYWNQGAERIYGWTAREAVGRTARELRLLSELNESQFEAGRQA
                     VMEKGEWTGELSQRTKAGAEITVESRWTLLRGDGGEPKSILVINTDATERKKVEGQLL
                     RAQRMESIGTLAGGIAHDLNNILSPILMAVQMLQVKVDDPGVLQWLEILQSNAERGAD
                     MVKQVLSFARGVEGERVALQPKHLVKEVIKILKDTLPKSIEIKFDVAPDLWTVSADAT
                     QVHQVLMNLCVNARDAMPRGGELFIRAENTTLDENFARMHIEAKPGRFVTVSVTDTGE
                     GMTPEVLSRVFEPFFTTKEVGKGTGLGLSTALTIVRSHGGFINVYSEPGKGTRFTIYL
                     PAIEGPAATAERKRLDLPEGRGELILVVDDEEAIRQITKGTLETFNYRVIVAGEGTEA
                     VALYAQNREEVAAVITDMMMPFMDGPATIRALHKMNPQVRVIAASGLGAGDKAAEAKA
                     AGAHIFLPKPYTAERLLKALAELLQEP"
     gene            3566..4033
                     /locus_tag="VF240_10450"
     CDS             3566..4033
                     /locus_tag="VF240_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7175668.1"
                     /translation="MKILLATDGSPCSEVAVAEVASRPWPADTQVRALSIFEPPAPLV
                     AEPYMVSAGYFEEAGKVIRQQAQEAVERAAATLRGGEKGGLQVSTEVLTGSAKRTIVE
                     EAEAWGADLIVVGSHGYKSWERMLLGSVSQAVAAHARCSVEIVRCNKEEEVRG"
     gene            4108..5232
                     /locus_tag="VF240_10455"
     CDS             4108..5232
                     /locus_tag="VF240_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7175669.1"
                     /translation="MANVLILGGGFGGVVAAERLAKALGPEHQLTLVSRSRRFIFYPA
                     LVRLAFGKATADDISYDLREAMLDRYVRFVEATAARVDPFARKVTLAGGDIVGELKYD
                     YLLFALGRRLATERVPGFFEHAHHLLSVEAAARFGEAVKEFKGGHAVIGSCPGARLDV
                     PVYETAFALRRRLEEQGTQARITLLRPDHPSTSPGGSDLARALRRALDSHHIETLSDF
                     PIAEVKAGLVRAQDGRELACDLLMLVPPFEGPSSLAATGLTDDEGFVRVDDTMRVLGA
                     ERMYAAGDALYFSGPKMGHMAVRQAEVAAENLAAEIAGREPAAHYKHELSLIVDAGGG
                     ETTYLHKSLWDDDGGKTVGHGRFWGWAKRAHEKYFQALHS"
     gene            5362..5796
                     /locus_tag="VF240_10460"
     CDS             5362..5796
                     /locus_tag="VF240_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7175670.1"
                     /translation="MLEVKEMSQGETLALLLRGKLGHLGCSRDGHPYVVPMNYAYDAE
                     CLYFFTTEGTKTEYMAANHEVCFQVEEIADATRWSSVMALGRAERVTKPEETERAMRL
                     IVERNPDLTPALNETKIGAWKRLNHIAIYRVRPHSIHGRKTA"
     gene            5826..6248
                     /locus_tag="VF240_10465"
     CDS             5826..6248
                     /locus_tag="VF240_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7175671.1"
                     /translation="MIERMSKEESHALLGSQRLARLGCVAEGEPYVVPINYVFDDGCA
                     LSHSLPGRKINAMRESPRVCLQVDEVADQLHWKSVLAFGTFEEVSSPDERARAFNLLL
                     SLFPRLTPVESRIAEDAAAPPPIVYRIRIDRVTGIREG"
     gene            6540..7505
                     /locus_tag="VF240_10470"
     CDS             6540..7505
                     /locus_tag="VF240_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7175672.1"
                     /translation="MNAKMKILIGYDGSPCADAALDDLQRAGLPPEGVEALVLAVAEV
                     WLPPPPPSIHEIAEAAEKIGAPAELQKWYARGSQAVREAEESAARAGARLRAKFPSWE
                     VTTEAEYGSPAWELIGRADQWKPDLVVVGSQGRTALGRFILGSVSQKVLTEARTSVRV
                     ARGRVQVEPSPVRVVVGVDGSPGSEAAVRAVASRRWPLRSEVRVCIVQDPLVPTLVGH
                     IIPPVAKWIEGANEAESGWLQKIADGARQRLSSAELIASTSVLAGDPKRVLVEEAEAW
                     GADCIFVGSMGFSNRVERFLIGSVSSAVAARAHCSVEVVREQKPE"
BASE COUNT         1381 a         2545 c         2496 g         1182 t
ORIGIN      
        1 gctgctcgac atcgacctgc cggacggctc cggcctcgac gccctgcgcg agattaagaa
       61 ccgacagccc gaggccgtcg tcatcatgat cacggccaac gtgctcatcc agaacacgat
      121 ctcggccctg cgcggcggcg catacgactt cgtcggcaag ccgctcaacc tcgaagagct
      181 gcgcgtcacg atccgcaacg gcatcgaggc gagccggctc cggcgcgagg tcacgcaggt
      241 gcgccgcgag cgcgcgcggc agttcagctt cgaccagatc atcggcgaca gcccggccgt
      301 caagcggatg ctctcgctcg cgcgcaaggt tgccgagtcg gaagcctcaa gcgtgctgtt
      361 gcagggcgag tcgggcacgg gcaaagacct cgtcgcgaag gccatccact acggctcgcg
      421 ccgggcggat gcgcccttcg tcgccatcaa ctgcgcggcc atcccctcga ccttgatcga
      481 gtcggagctg ttcggctacg agaagggggc gttcaccgac gcgaaggcgc gcaaggaggg
      541 actgttcgag caggccgaag gcggcacgct cttcctcgac gagatcggcg aactcgaatt
      601 gggcttgcag gcgaagctgc tgcgcgtgct cgaagagggg agcttccgtc gggtcggcgg
      661 actcaaagac atccccttag acgtgcgtgt ggtcgccgcg tcgaaccgcg acctcaagac
      721 ggagggcgag gccggccgct tccgcgttga cctctactac cgcctgtcgg tgattcagat
      781 cgacatcccg cctttgcgcg ggcgcggcga cgacaccttg ctgctcgccg agcactacgt
      841 caaaaccttc ggcgagcgcc tgcgtctgaa aaataagatt cgcggcctct cgcccgaggt
      901 gcgggagatt tttcttcaat acgcgtggcc cggcaacgtc cgcgagctgc gcaacgtcat
      961 cgagcgcgcc ctcatcctcg aagacggcga cctcgtcacg atggagtatc tgccacgcga
     1021 cctgacgggg cgaacggagc ctgtggcctc gcccggcgcg gagggcggcg gcgcggcgca
     1081 cctcttccgg ctcccgcccg acggcgtgcc gctcgaagag gtggagaact cgctcgtgcg
     1141 tcaggccgtc gagcgcagca acggcaacca gacgcgcgcc gccgaactcc tccgcatctc
     1201 gcgcgaccag ttgcgctacc gcctcaagaa gctcgaaagc cccgctccgc atgacaagac
     1261 cgcgaccgaa gcggcctcct gaagtccgta aacccacgat gagcgaaagt accctgcacg
     1321 agactacgca aacgggcgag cgccccttcg acaccgagga agcgctcagg cgctcgctca
     1381 aggagctggc cgacctgaag ttcgccctcg acgagtcggc catcgtcgcc atcaccgacc
     1441 agcgcggcgt catcaactac atcaacgaca agttctgcca aatctcgaag tactcgcgcg
     1501 aagagctgtt gggccaggat caccgcatca tcaacagcgc ctaccacccc gaagagttca
     1561 tccgcaacct ctggacgacc atcgcctccg gtcaggtctg gcgcggcgag atacgcaacc
     1621 gcgccaagga cggctcgatc tactgggtgg acaccaccat cgtcccgttc ctcaacccgg
     1681 aggggaagcc ctaccagtac gtcgccatcc gctacgacat caccgagcgc aagcgggccg
     1741 aggagcgcat cggcgagcag gcggccctgc tcgacaaggc gcaggacgcc atcgtcgtgc
     1801 gcgacgcggg cgacaacaaa atcctctact ggaatcaggg cgccgagcgc atctacggtt
     1861 ggacggcccg tgaggccgtg ggccgcaccg cgcgcgagtt gcgtctgctg agtgagctga
     1921 acgagtctca gttcgaggcg ggccgtcagg ccgtgatgga gaagggggag tggacgggcg
     1981 aactgagcca gcgcacgaag gcgggcgccg agatcacggt cgagagtcgc tggacacttc
     2041 tgcgcggcga cggcggcgag ccgaagtcga tcctcgtcat caacacggac gcgaccgagc
     2101 gcaagaaggt cgaggggcag cttttgcgcg cgcagcggat ggagtcaatc gggacgctgg
     2161 ccggcggcat cgcgcacgac ctcaacaaca tcctctcgcc catcctgatg gccgtgcaga
     2221 tgctccaggt gaaggtggac gaccccggag tgctccagtg gctggagata cttcagtcga
     2281 acgccgagcg cggcgccgac atggtcaagc aggttctatc gttcgcgcgc ggagtggagg
     2341 gcgagcgcgt cgcgctccag ccgaagcacc tcgtcaagga agtgatcaag attctgaagg
     2401 acacgctgcc gaagtccatc gagatcaagt tcgacgtggc gcccgacctc tggacggtct
     2461 cggccgacgc cacgcaggtg catcaggtct tgatgaacct ctgcgtcaac gcgcgcgatg
     2521 ccatgccgcg cggcggcgaa ctttttatcc gcgccgagaa cacgacgctc gacgagaact
     2581 ttgcgcgcat gcacatcgag gccaagccgg gccgcttcgt caccgtctcc gtcacagaca
     2641 cgggcgaggg gatgacgccg gaggtcttga gccgcgtctt cgagccgttc ttcacgacga
     2701 aggaggtggg caagggcaca gggctcggac tctcgacggc cctgaccatc gtccgcagcc
     2761 acggcggctt catcaacgtc tacagcgagc cgggcaaggg cacgcgcttc accatctacc
     2821 tgccggccat tgaggggccg gccgcgaccg ccgagcgcaa gcgcctcgac ctgccggagg
     2881 gtcgcggcga gttgatcctc gtcgtagacg atgaggaagc catccgccag atcacgaagg
     2941 gcacgctcga aaccttcaac taccgcgtca tcgtcgcggg cgaagggacg gaggccgtgg
     3001 cgctctacgc gcagaaccgc gaagaggtcg ccgccgtcat caccgacatg atgatgccct
     3061 tcatggacgg ccccgccacc attcgcgcgc tccacaagat gaacccgcag gtgcgcgtca
     3121 tcgccgccag cgggctgggg gcgggcgaca aggccgccga ggccaaggcc gccggcgccc
     3181 acatctttct gcccaaacct tacacggcgg aaagactcct gaaggccctc gccgaactcc
     3241 tccaagagcc gtgataagtg atgagtgata agtgataaga aggaagcata gccgtcagct
     3301 atcaactctt agctaacagc taaggctaat cgctgacagc tcttatcact tattacttat
     3361 cactcatcac tcatcactac tggggaattg tccacacggc ggcgcaaaag cacccacgtc
     3421 gtcgccgcgc ataccagcga tttatcacga aactgacctc gaaaggcgtt ggcacggccc
     3481 gtgcagacag ggggcgtggg ctgccggggc gcacgcgccg acgcggccga tgagctttga
     3541 cttgttcccg agggaggcga aagcgatgaa gattctactg gctaccgacg gctcgccgtg
     3601 cagcgaagtg gccgtcgcgg aagtggcaag caggccgtgg cccgcggaca cgcaggtgcg
     3661 cgccctctca atcttcgagc cgcccgcgcc gctcgtcgcc gagccgtaca tggtttcggc
     3721 cggctacttc gaggaggccg ggaaggtgat tagacagcag gcgcaggagg cggtcgagcg
     3781 ggccgccgcg acgctacggg gcggcgagaa gggcggcctt caggtctcga ccgaggtgct
     3841 gaccggctcg gccaagcgca ccatcgtcga ggaggccgag gcatggggcg ccgacctcat
     3901 cgtcgtcggc tctcacggct acaagtcctg ggagcgaatg ctgttggggt ctgtctcgca
     3961 ggccgtcgcg gcacacgcgc gttgctcagt cgagatcgtg cgctgcaaca aggaagaaga
     4021 ggtcagaggt taggtgtcag aggtcagtcg aagacaaagg cttttactaa cctctgacct
     4081 ccaacctctg acctctcgga tttcacaatg gcaaatgttc tcatcctcgg gggcggcttc
     4141 ggcggggtgg tggcggcgga gcggctggcg aaggcgctcg ggccggagca ccagctcacg
     4201 ctcgtctcgc gcagccgccg cttcatcttc taccccgcgc tcgtccgtct cgccttcggc
     4261 aaggccacgg ccgacgacat ctcttacgac ctgcgcgagg cgatgctcga ccgctacgtc
     4321 cgcttcgtcg aggcgacggc cgcgcgcgtc gatcccttcg cgcgcaaggt gacgctcgcc
     4381 gggggcgaca tcgtcggcga gctcaagtac gactacctcc tgttcgcgct cgggcgccgg
     4441 ctcgccaccg agcgcgtgcc cggcttcttc gagcacgctc accacctgct ctcggtcgag
     4501 gccgccgcgc gcttcggcga agccgtcaag gaattcaagg gcggacacgc cgtcatcggc
     4561 tcgtgccccg gcgcgcgcct cgacgtgccg gtctacgaga cggccttcgc gcttcgccgc
     4621 cgcctcgaag agcagggcac gcaggcgcgc atcaccttgc tccgccccga ccacccgagc
     4681 acgtcccccg gcggctccga cctcgcgcgc gccctgcgcc gcgccctcga ctcacaccac
     4741 attgagacgc tgagcgactt ccccatcgcg gaggttaagg cgggactcgt ccgcgcgcag
     4801 gacgggcgcg agcttgcgtg tgatttgttg atgcttgtcc cgcccttcga gggcccgtcg
     4861 tcgctcgccg cgacggggct gacggacgac gaagggttcg tgcgcgttga cgacacgatg
     4921 cgcgtgctcg gtgccgagag gatgtacgcc gccggcgacg ctctctactt ctcggggccg
     4981 aaaatggggc acatggccgt caggcaggcc gaggtggccg ccgaaaacct cgccgccgag
     5041 atcgcggggc gcgagccggc cgcacactac aaacacgaac tctcgctcat cgtggacgcg
     5101 ggcggcgggg agaccaccta cctccacaag agcctctggg acgatgacgg cggcaagacg
     5161 gtcggccacg gccgcttctg gggctgggcc aagcgcgcgc acgagaaata ttttcaggcg
     5221 ctacactctt aggaggtgat aagtgatgag tgacaagtga taagaatctg ttagctctta
     5281 gctgttggct aatcgctaac tgctaaccgc gtcctttctt tcttatcact tatcactcat
     5341 cactcatcac gggtttcaga gatgcttgaa gtcaaagaaa tgtcgcaggg cgagacgctg
     5401 gcgctgctgc tgagagggaa gttggggcac ctcgggtgct cgcgcgacgg ccacccttac
     5461 gtcgtcccga tgaactacgc ctacgacgcg gagtgcctct acttcttcac caccgagggc
     5521 acgaagaccg agtacatggc cgccaaccat gaggtctgct tccaggtcga ggagatcgcg
     5581 gacgccaccc gctggtcgag cgtgatggcg ttgggccgcg ccgagcgggt gacgaagccg
     5641 gaggagacgg agcgcgccat gcgactcatc gtcgagcgca accccgacct cacgcccgca
     5701 ctcaacgaga cgaagatcgg agcctggaag aggctcaatc acatcgccat ctaccgggtg
     5761 cgcccgcact ccatccacgg gcgcaagacc gcctgagacg cggcccggcc gcgaaggtga
     5821 ccgccatgat cgaaaggatg agcaaggaag agtcccacgc gcttctcggc tctcagcgcc
     5881 tcgcccgcct cggctgcgtc gccgagggcg agccttacgt cgtccccatc aactacgtct
     5941 tcgacgacgg gtgcgccctc agccactcgc tccccggccg caagatcaac gcgatgcgcg
     6001 agagtccgcg cgtctgcctt caagttgacg aggtggccga ccaactccac tggaagagcg
     6061 tgctggcttt cgggacgttc gaggaggtga gcagccccga cgagcgagcg cgggccttta
     6121 acctcctgct ctcactcttc ccgcgcctca cgcccgtcga gtctcgcatc gcggaggacg
     6181 ccgccgcgcc cccgccaatc gtctaccgca tccgcatcga ccgcgtcacg ggcatccgcg
     6241 aaggatgagc cgccgccatc cgccacgaca ctgaagtgca aatgcgccgt cgtccctccc
     6301 ggcgacggtt gtgtaaaact ccgcaccgcc acgcaaaatt cagcacacgc gcgcgcccga
     6361 caccggcaaa tatcagacct tctcttccgg catatctgtt gccgtcacac cgggcaggag
     6421 accacccgcc actcttgcac ggggcgtgcg cgcctgagac ggccggcggc ggacacgccc
     6481 ctatcgtcgg ccgggtcgcc cttgtttggc gctccgttgc gcgccgggag gtttgagtta
     6541 tgaacgcgaa gatgaaaatt ctgatcggct acgacggctc accttgcgcc gacgcggcgc
     6601 tcgatgactt gcagcgggcc gggctgccgc ccgaaggcgt cgaggcgttg gtgctggccg
     6661 tcgccgaggt gtggctcccg ccgcccccgc cctcgatcca cgaaatcgcc gaggcggccg
     6721 aaaagatagg ggcgcccgcc gagcttcaga agtggtacgc ccgaggctct caggcggtca
     6781 gggaggcgga ggagtcggcc gcgcgtgccg gcgcgaggct gcgtgccaag ttcccctcct
     6841 gggaggtcac gaccgaggcg gagtatggct cgccggcctg ggagctaatc gggagggccg
     6901 accagtggaa gcccgacctc gtcgtcgtcg gatcacaggg ccggacggca ttggggcgct
     6961 tcatcctcgg cagcgtctcg cagaaagtgc tgaccgaggc gcgcacgtcc gtgcgtgtcg
     7021 cgcgcggccg cgttcaggtt gagccgtcgc cggtgcgtgt cgtcgtcggc gtggacggct
     7081 ctcccggctc cgaggcggcg gtgcgcgccg tggcgtcgcg ccggtggccc ctgaggagcg
     7141 aggtgcgcgt ctgtatcgtc caagacccgc tcgtcccgac cctggtcggg cacatcatcc
     7201 cgccggtggc gaagtggatc gagggggcta acgaggccga gtcagggtgg ttgcagaaga
     7261 tcgccgacgg ggcgcggcag cgcctgtcct ctgccgaact catcgcctcg acctcggtgc
     7321 tggcgggcga tccgaagcgg gtgctggtcg aggaggccga agcgtggggc gctgactgca
     7381 tcttcgtcgg ctcgatgggc ttcagcaacc gcgtcgagcg gttcctcatc ggcagcgtgt
     7441 cgtcggcggt ggccgcgcgc gcccactgct cggtcgaagt cgtccgcgag caaaagcccg
     7501 agtagagcgc tccggccgac ggcgaacact gagttgaagc cgacgggcgc aggttacgac
     7561 gggaggcaca gatggcaacg atgagggcga cggtttttca cggc
//
LOCUS       DASVES010000259         5325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1212472, whole genome shotgun sequence.
ACCESSION   DASVES010000259 DASVES010000000
VERSION     DASVES010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212472"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VF240_10475"
     CDS             <1..462
                     /locus_tag="VF240_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175673.1"
                     /translation="FGWPYCYFDPQQRKHVLAPEYGGDGGKAQGVCAQKKQPDLTFRA
                     HWAPNGMTFYRGSTFPAKYRGGAFVAFHGSHDRTPTQAGYRVAFVPFEAAKPAGRYEE
                     FATGFAGAGPLAAPTEAPHRPMGVAVGPDGALYVSDDVKGRIWRITYVGGV"
     gene            636..1895
                     /locus_tag="VF240_10480"
     CDS             636..1895
                     /locus_tag="VF240_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX7175674.1"
                     /translation="MFILADDLGYADLGCYGGREDCSPNLDRMATEGLIFTNGYANSC
                     ICSPSRFAIITGRYQHRLRGGFDEPIGSVSPELGLPPEQPTMASLLRDAGYATALVGK
                     WHLGSLPWFSPLKSGYDEFFGHRTGAVDFFSHAGFGGHDLWEGDEEVFKEGYLTDLMT
                     DRAVDFVKRQSAEKPFLLSLHYNAPHWPWETREDEEESKRIGNKIAHLDGGSVKTYLE
                     MVRVMDEGIGRVLEALDETGAGENTLVVFTSDNGGERFSDTYPLMGKKYDLLEGGIRV
                     PYVVRWPAKLPAGETYDRLAIGMDWTATFIAAAGVTAHADYPLDGIDLFGDEVERNLY
                     WRMIYRNQKAVRSGVWKYLSIEGNEFLYDLSRDERERANMRYREPEKFEELRKAYAEW
                     DASMPPRPEDAEVHLEYTAATMAKSDG"
     gene            1998..3794
                     /locus_tag="VF240_10485"
     CDS             1998..3794
                     /locus_tag="VF240_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX7175675.1"
                     /translation="METTPTSDIEPEVNQPGLRDLREAVANHLRRRLPGRASLRDDGI
                     AGLNSAVSSVPDGMASGILVGVNPIYGLYACMVGPIAGGLFSSTRLMIIATTSAASLS
                     AGQALGGLQGEARDSALFLMVVLIGALQVLFGLSRTGQLTRFVSYSVMTGFIIGIAVL
                     TILSQLPTVAGYEPTAGNNKVTQTFNLLSNLDRVSLWSLAVAVTTLVLAVTLPRTRLG
                     NYGRLAAIIVPSVLVALFSMDSVAVVRDIGEIPGGIPTPHLPSFSGLSFDVVTGALAV
                     SVIILVQGAGVSQSVPNPDGSRRSTSRDFIAQGAANVASGFFRGLPVGGSLSTTALTV
                     ISGARTRWAAIFAGLWMALLVVGFPGLVSYVAMPALGVLLILASVSTIKPSEALSIWN
                     TGWPSRLASVTTFLSTLLLPIQAAVGIGVVLSALLYVNESSTDISVVELVKRDDGLIE
                     EHKAPKQLQSNKVTVLDVYGHLFYAGARTLERLLPKPRDVQRPVVILRLRGRTTFGAT
                     LLEVLSNYADKLQEANGRLYLTGISEGAREQVVRTGKFGPSGPVQAYEATAVRGQSTE
                     EAVADARTWLVSKSADASPDDASSSTREGEAG"
     gene            3791..>5325
                     /locus_tag="VF240_10490"
     CDS             3791..>5325
                     /locus_tag="VF240_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX7175676.1"
                     /translation="MSAPVPLPEATTDTESRWSRLRKDTVAGLVNAVVSVPDGLACAA
                     LAGVNPVYGLYTSITAPIGGSLLVSAQLMQIATTSASALAAAQAIASYPAQQRDAALF
                     LLVAIVGVVLAVFGLLKLGRLVRFVSHAVMTGFLIGVAVVLVLDQLAPFVGFHPRGAN
                     EVVQFVDLLAHASQFSLPTIITGLVALGILFGLERTRLATLSSLFALVVPTLAVALIG
                     WESVESVAQVSPIPRGIPVPALPDLSLFTPQLVLSAFALAVVIAVQGAGVSQSVKNPD
                     GSPISVSRDMVAQGAANVASGLFSGIPAGGSVGQTALNVTVGARSRWAGVLHGLWMLV
                     IVLLVPGLVGQVPMAVLAALMIMAGISAINFREARSIWNTGGAARWSISVTFVATLVL
                     SIPLAVAAGVLLTVVLYLKSSASDVRVLELVRLDDGRIAASKPPARLPGNKVTVLDVD
                     GSLFFAGARTLSEALPTPAGATRPVVVLRLRGYTSVGATLVEVLDDYADALAEVGGRL
                     YLSG"
BASE COUNT          895 a         1672 c         1763 g          995 t
ORIGIN      
        1 ttcggctggc cctactgcta cttcgaccct caacagcgca agcacgtgct cgcgccggaa
       61 tacggcgggg acggcgggaa ggcgcagggc gtctgcgcgc agaagaagca acccgacctg
      121 accttccgcg cgcactgggc tcccaacggg atgacgttct atcgcggctc gacttttccg
      181 gcgaagtacc gcggcggcgc ctttgtggcc ttccacggct cccatgacag aacgccgacg
      241 caggcgggct atcgggtggc gttcgtgccg ttcgaggcgg ctaaaccggc gggccgctac
      301 gaagaattcg cgacgggttt tgccggcgcg gggccgcttg cggctcctac cgaagcgccc
      361 caccggccca tgggagtggc ggtcggccct gatggggcgc tgtacgtctc cgatgacgtc
      421 aaagggcgga tttggagaat cacttacgtc ggcggcgtct gacctcggtt gcgagtgaag
      481 gcgaagggtg agagccgcct ttcgccttca ctaaatagag gagaggaatc acgcgactga
      541 ctgcatgagt gcttattcac cgggaaggag ttgatatgag tacgagggag acgaagaccg
      601 atgcgcgcgg gagtgtttcg acgaggccga attttgtgtt catcctggca gacgatctcg
      661 gctacgcaga cctcggctgc tacggcggca gagaagactg ttcgcctaac ctcgaccgca
      721 tggcgaccga gggtttgatt tttaccaacg gctacgccaa ctcgtgcatc tgctcgccgt
      781 ctcgatttgc gattatcacg ggacgctacc agcatcggct tcgcggcggc tttgacgagc
      841 cgattggcag cgtttcgccc gaactcggtt tgccgcccga acagccgacg atggcatcct
      901 tgctgcgcga cgcgggttac gctaccgcgc tcgtggggaa atggcatctg ggttcgctgc
      961 cctggttcag cccgctcaaa agcggttacg acgagttttt cggacaccgc accggggcgg
     1021 tcgatttttt ctcacacgcc ggtttcggcg gacacgatct ttgggaaggc gacgaggagg
     1081 tatttaagga gggctacctg accgatctaa tgaccgaccg ggcggttgat tttgtgaaac
     1141 gccagtcggc ggaaaaaccg tttctgctct ccttgcatta caacgcgccg cactggccgt
     1201 gggagacgcg cgaagacgaa gaagagtcga aaagaatcgg gaataaaatc gcgcatctcg
     1261 acggcggctc ggtgaaaact tatctggaaa tggttcgcgt gatggatgaa ggcatcgggc
     1321 gcgtccttga ggcgctcgac gaaacgggag cgggcgaaaa cacgctcgtc gtttttacga
     1381 gcgacaacgg cggcgaacgc ttctctgaca cctacccgtt gatgggcaaa aagtatgacc
     1441 tgctcgaagg cggcatccgt gttccttacg tcgtgcgctg gcctgccaag ctccccgccg
     1501 gtgaaaccta tgaccgtctc gccatcggga tggattggac ggcgactttt atcgccgccg
     1561 ccggtgttac ggcgcatgcc gattacccgc tcgacggcat tgatctgttc ggcgacgaag
     1621 tcgagcgcaa tctttactgg cgaatgattt accgcaatca aaaggcggtg cgttcgggcg
     1681 tctggaaata tctttccatc gaaggcaacg agttccttta cgatttgtcg cgcgacgagc
     1741 gcgagcgcgc gaacatgcga tatcgagagc cggagaagtt cgaggaactg cggaaggcgt
     1801 atgccgaatg ggacgcgagt atgccgccgc gacccgaaga cgccgaggtt catttagaat
     1861 acaccgccgc aacgatggca aagtcggacg gttagttccg agcgaaatcg cttccgaaag
     1921 tcttccgctg tggacgcact cgcatctcca cgcgcccaca gcatcgcgac ttatgacagg
     1981 gcgatcagga tgaatagatg gagacgacgc caacatccga catagagccg gaggtcaacc
     2041 aaccgggact tcgcgatttg cgcgaggcgg tggctaacca cctccgccgg cggctgccgg
     2101 gtcgcgcgtc gctccgcgat gacggcatcg ccgggttgaa cagcgccgtc agcagcgtgc
     2161 ccgacgggat ggcgagcggc atcctggtgg gtgtcaaccc gatctacggc ctctacgcct
     2221 gcatggtggg gccgattgcg ggcggcctct tctccagcac ccggctgatg attatcgcca
     2281 ccacgagcgc cgcgtcgctc tccgcgggtc aggcgctcgg cggtctgcaa ggagaagcgc
     2341 gcgacagtgc cctgttcctc atggtggtgt taatcggcgc cttgcaggtt ctgttcggac
     2401 tgtcgcggac ggggcagttg acccgcttcg tctcttactc ggtgatgacc ggcttcatca
     2461 tcggcatcgc ggtgctgacg atactcagcc aactcccgac cgtcgcaggc tacgagccga
     2521 cggcgggtaa caataaagtc acacagactt tcaacctgct gtccaacctc gaccgcgtca
     2581 gcctgtggtc gctcgccgtg gcggtcacca cccttgtgct cgccgtcacg ctgccgcgca
     2641 cacggctcgg caactacggc aggctggcgg cgattatcgt cccgtccgtg ctcgtcgcgc
     2701 tcttcagcat ggacagcgtg gccgtcgtcc gagacatcgg cgagattccc ggcggcatac
     2761 cgacgccgca ccttccgtcg ttctccggcc tttcgttcga cgtggtgacg ggcgcgctgg
     2821 ccgtctcggt gatcatcctc gtgcagggcg cgggggtcag ccagagcgtg cccaacccgg
     2881 acggctcccg gcgcagcaca tcgcgcgact tcatcgcaca gggggcggcc aacgtcgctt
     2941 cgggtttctt ccggggtctg ccggtgggcg gctcgctgag cacgacggcg ctgaccgtca
     3001 tctcgggagc gcgcacacgt tgggcggcga tcttcgcggg attgtggatg gcgctgctcg
     3061 tggtgggctt ccccggcctg gtgtcttacg tggcgatgcc cgcgctcggc gtgctgctga
     3121 tcctcgccag cgtcagcacc atcaagccgt cggaggcgct ctcgatctgg aacacgggct
     3181 ggccgtcacg cctggcgagc gtcaccactt tcctctcaac gctcctcctg ccgattcagg
     3241 cggcggtcgg catcggggtc gtcctctccg ccctgctcta cgtgaacgag tcctcgaccg
     3301 acatctcggt ggtcgagctg gtgaagcggg acgacggatt aatcgaagaa cacaaggccc
     3361 ccaagcaact acagtcgaac aaggtgacgg tgctcgatgt ctacgggcac ctcttttacg
     3421 cgggcgcgcg gacgctggag cggctactgc ccaagccccg agacgtacag aggccggtgg
     3481 tgatcctgcg tttgcgcgga cgcaccacct tcggcgcgac ccttctggaa gtgctgtcca
     3541 actatgccga caagctacag gaagcgaacg gccggttgta tctcacggga atcagcgagg
     3601 gggcgcggga acaagtggtg cggacaggca agttcggccc gagcggcccc gtgcaagcct
     3661 acgaagcgac tgccgtccgc gggcaatcga cagaggaggc ggtcgccgac gcgaggacgt
     3721 ggctggtcag caagagcgca gacgcctcgc ccgacgatgc gtcaagctca acgcgcgaag
     3781 gtgaagccgg atgagcgcgc ccgtcccgct ccccgaagcg acgaccgata ccgagtcgcg
     3841 gtggagcagg ctccgtaaag atacggtcgc cgggctcgtc aacgccgtcg tgagcgtccc
     3901 cgacgggctg gcctgcgccg ccctcgccgg cgtcaatccg gtttacggac tctacaccag
     3961 catcaccgcg ccgattggcg gaagcctcct ggtgagcgcg caactgatgc agatcgctac
     4021 caccagcgcc tctgcattgg ccgccgcgca agccattgcg agctatccgg cgcagcagcg
     4081 cgacgcggca ctgttcctgc tggtcgcgat agtcggcgtg gtactcgccg tctttgggct
     4141 gctgaagttg ggacggctcg tgcgcttcgt ctcgcacgcc gtgatgaccg gcttcctgat
     4201 tggcgtggcg gtcgtccttg tactcgacca actcgccccc tttgtcggct tccacccgcg
     4261 cggcgcgaac gaggtcgtgc aatttgttga cctgctggct catgcctccc agttcagcct
     4321 gccgacgatc atcacgggac tcgtcgcgct cggcatcttg ttcgggctgg agcgcacacg
     4381 gcttgccaca ctgtcttcac tcttcgcgct cgtcgtgccg acgctcgcgg tggccctgat
     4441 aggttgggag agcgtcgaga gcgtcgccca ggttagcccc atcccgcgcg gcatccccgt
     4501 gccggcactg cccgatctgt cgctgttcac gccccagttg gtcttgtcgg cgttcgccct
     4561 cgcggtggtc atcgccgttc agggagccgg ggtgagccag agcgtgaaga acccggacgg
     4621 gagcccgata agtgtgtcgc gggacatggt ggcgcagggc gcggcgaacg tcgcgagcgg
     4681 gcttttttcc ggcatcccgg cgggcggctc ggtcgggcag acggcgctca acgtgacggt
     4741 cggcgcgcgc agccgctggg cgggcgtgct gcacggcttg tggatgctcg tcatcgttct
     4801 gctcgtgccg ggccttgtag gtcaggtgcc gatggcggtg ctggccgcgc tgatgatcat
     4861 ggcgggcatc agcgccatca acttccgcga ggcacgttcg atctggaaca cgggcggggc
     4921 ggcgcgctgg tcaatctcgg tgaccttcgt ggcgacgctg gtgctctcta tcccgctcgc
     4981 ggtcgccgcc ggcgtcctgc tgaccgtcgt cctctatctc aagtcgtcgg cgagcgacgt
     5041 gagggtgctg gaactcgtcc ggttggacga cgggcgcatc gccgcgagca agccgcccgc
     5101 gcgactgccg ggcaataaag tgacggtgct tgacgttgac gggagtctgt tcttcgccgg
     5161 ggcccgcacg ctctcggagg cgctaccgac accagcgggc gcgacgcgcc ccgtcgtcgt
     5221 cttgcgcctg cggggataca cgagcgtggg cgcgacgctc gtcgaggtgc tcgacgacta
     5281 cgccgacgcg ctcgccgaag tgggcgggcg gctttacttg agcgg
//
LOCUS       DASVES010000260         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_492123, whole genome shotgun sequence.
ACCESSION   DASVES010000260 DASVES010000000
VERSION     DASVES010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492123"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VF240_10495"
     CDS             <1..724
                     /locus_tag="VF240_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175677.1"
                     /translation="FMFIFPTAYFFHTGYTESVFMPLVFGALLAARKERWALAGVLGA
                     FAALTRVNGMLLIPVLAVEAFMQYRREGGGRFRREWLWMLLTSLGFLVYLSINYSVWG
                     DAFYFQKVLEKYWYKKLTWPWVGIGGTLNDALNRSPAEAHMLGVQEFFFIALGAVCTV
                     WAWRRLRPSYAVWMTLNWLLVTSTSHVMSTPRYMLGMFPIFLIFARLAEARPNWGAII
                     TVWSLTFLGIFTALFVQGHWAF"
     gene            1046..2089
                     /locus_tag="VF240_10500"
     CDS             1046..2089
                     /locus_tag="VF240_10500"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX7175678.1"
                     /translation="MKRMLTVVMALSAAVVFAVLFWPSRTGAQREQGRASNVDPKPLR
                     PVHTYSIVARDPATGELGVAVQSHWFSVGQLVTWAEAGVGAVATQSFVDPSYGKLGLD
                     LMRAGRSAPDTLKSLVAGDDGRDVRQVAMIDVRGHVAAHTGAKCIDAAGHHVGKDYSV
                     QANMMLNERVWPAMSRAFEETRGDLAERMMAALDAAQSVGGDIRGKQSAAIVIVSGKS
                     TGRPWADKTFDLRVDDHAEPLRELRRLIGLQRAYNHMNAGDLAVERKDNAGALREYSA
                     AQKLVPDNLEMAYWHAVALVGMNRTDEALPIFRRVFSADPNWRTMTPRLVKSGILPND
                     PKLIERITREGQK"
BASE COUNT          383 a          693 c          686 g          418 t
ORIGIN      
        1 ctttatgttc atcttcccga ccgcctactt ctttcacacc ggctacaccg agagcgtctt
       61 catgccgtta gtgttcggcg ctctgctggc ggcgcggaag gagcgttggg cgctggccgg
      121 ggtcttaggc gctttcgccg cgctcacgcg cgtcaacggg atgctgctca tcccggtgtt
      181 ggctgttgag gcgttcatgc agtaccggcg cgagggcggc ggacggttcc ggcgcgagtg
      241 gctgtggatg ctgctcacgt cgctgggctt cctcgtctac ctctcgatca actacagcgt
      301 gtggggagac gcgttctact tccagaaggt tctggagaag tactggtaca aaaaactgac
      361 gtggccgtgg gtcggcatcg gcggaacact taacgacgcg ttgaatcgca gcccggccga
      421 ggcgcacatg ctcggcgtgc aggagttctt cttcatcgcg ctcggggcgg tgtgcaccgt
      481 ctgggcgtgg cggcggctgc gtccgtccta cgccgtctgg atgacgctca actggctgct
      541 ggtgacaagc acctcgcacg tcatgagcac gccgcgctac atgctcggca tgtttcccat
      601 cttcctcatc tttgcccggc tcgcggaggc gcgtcccaac tggggcgcga tcatcaccgt
      661 ctggtcgctc accttcctcg gcatcttcac ggccctgttc gtacagggtc actgggcgtt
      721 ttagagtggg acgagtgaag aagagtgtga gtgctttcaa agcgcgttgc aaaactttag
      781 gggcagggca agtccgcggc ccgcttccgg caaagggaat taacatggat ggacaggatt
      841 gacaggatga agaaagagct gatttgagat ttgagatttc agatttcaaa tcgtctcgtc
      901 ctgtccgtcc tgcaaatcct gtccgtccat gttaattgtt ttagcaagcg cgggccgcgg
      961 acttgccctg cccctacagt cgtgacgcac gccgagtggt cttgcaacgg actctttgtt
     1021 ttcaattgaa cgcagaggtg agacgatgaa aagaatgctt accgtagtga tggcactttc
     1081 ggcggcggtc gtgttcgccg tgctcttctg gccttcgaga accggcgcgc agcgggaaca
     1141 ggggcgagca tcgaatgttg atccgaagcc cctgcggccc gtccacacct actcgattgt
     1201 cgcgcgcgac ccggcgacgg gcgagctggg cgtggccgtg cagtcgcact ggttctccgt
     1261 cgggcagctc gtgacgtggg ccgaggcggg cgtcggcgcg gtcgccacgc agagcttcgt
     1321 cgatccgtcc tacggcaagc tcggactcga cctgatgcgc gccggccgca gcgcccccga
     1381 cacgctcaag tccctcgtcg ccggcgacga cggccgcgac gtgcggcagg tggcgatgat
     1441 cgacgttcga gggcacgtcg ccgcgcacac cggcgcgaag tgcatcgacg cggcgggcca
     1501 ccacgtcggc aaagattatt ccgtgcaggc caacatgatg ttgaatgaac gcgtgtggcc
     1561 cgccatgtcg cgcgccttcg aggagacccg aggcgacttg gccgaacgca tgatggccgc
     1621 cctcgacgcg gcgcaatccg tcggcggcga catccgcggc aagcagtcgg cggccatcgt
     1681 catcgtctct ggcaaatcca cgggccgccc gtgggccgac aagaccttcg atctgcgcgt
     1741 ggacgaccac gcggaacccc tgcgcgagct gcgccgcctc atcggcttgc agcgcgccta
     1801 caaccacatg aacgcgggcg acctcgccgt cgagcgcaag gacaacgccg gggcgctgcg
     1861 cgaatactcg gcggcgcaaa aactcgtccc cgacaacctt gagatggcct actggcacgc
     1921 cgtcgccctc gtcggcatga accgcaccga cgaagccctc cccatcttcc gccgcgtctt
     1981 ctccgccgac cccaactggc gcaccatgac cccgcgcctc gttaaatccg gcatcctccc
     2041 caacgacccg aagcttatcg agcgcatcac tagagaaggg caaaagtaaa aaggcaaaag
     2101 gcaaaagtgg gggagcagcg ttcgcgcaca tcttggcagg cacgtgcctt gtcccgcctt
     2161 cgcgggcgtg ttactttcgt
//
LOCUS       DASVES010000261         8682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_606257, whole genome shotgun sequence.
ACCESSION   DASVES010000261 DASVES010000000
VERSION     DASVES010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606257"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF240_10505"
     CDS             <1..406
                     /locus_tag="VF240_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX7175679.1"
                     /translation="VYLPVFHEGALFYFGDGHALQGDGEVCGSGLETSMDVVFHFELR
                     KGKRIRWPRIENDEYIMATGSVRPLMDAFRIAQIELIDWLVADYGFDKWEALQVVSQV
                     GTTRVANAVDPNYTVVAKFPKKYLPRPGAPSN"
     gene            complement(589..2736)
                     /locus_tag="VF240_10510"
     CDS             complement(589..2736)
                     /locus_tag="VF240_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7175680.1"
                     /translation="MSTAFAPARRRAPFLFILLLAAFVPRAAGQSFTMEQVRGYPFPN
                     ELTASAAGSRVAWAFNERGRRNVYVAEGPDFRPRRLTNYDADDGQEITSLQVSSDGRF
                     VVYVRGGEHSSNWDDTVPVNPASSPVATKVQIWSVAFAGGEPKLLADGDEPVVSPKGD
                     RVVFAKDRGLWVAPTDGSTQAKKMFSARGEPGSPVWSPDAARLAFISSRGDHAFVGVY
                     TNDSQPILWLAPSTSRDSSPRWSPDGRSVAFVRRPGAGGAPEAILEQRPQGWAIWTAD
                     AATGEGRQLWKSPNSLRGSPPSTHGGVNLHWAAAGRIVFLSYMDGWPHLYSIPERGGE
                     PTLLTPGDYMAEHVSISPDRRHLVFAGNAGRGADDIDRRHVVRVSVDRAAPEVLTPGD
                     GLEWAPVVTGDGRHVVLISATPQRPPLPAVVPWAGGPLRVLAEDRVSADFPAAHLVTP
                     RKVVYKAPDGVQVHAQLFERPGGGGAAKKPAVVYVHGGPPRQMLLGWHYSDYYSNAYA
                     LNQYLASRGYVVLSINYRLGIGYGYDFHRPASAGAQGAAEYQDIKAAGEYLRSLPQVD
                     GKRIGVYGGSYGGFLTALALARDSDLFAAGVDIHGVHNWTAERAQSLLEGRYEKAPDT
                     QAALDIAWNSSPVASVSKWRSPVLLIHGDDDRNVRFSQTVDLARRLERAGVPFEELII
                     PDDTHHFMRHANWLRVNTATARFFDKVFGAGLP"
     gene            complement(3011..4057)
                     /locus_tag="VF240_10515"
     CDS             complement(3011..4057)
                     /locus_tag="VF240_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175681.1"
                     /translation="MPYVQHTHCVPTQDYTGLGSAVGIAGGILAVLGIILASFFGGGL
                     VGAALAAAGGMMAIFDVCNYLLGGKLICLGHDMCAIGRVVGLEPPGFNKPFPENIDND
                     YSVNILLSPHLPSATLAQQQNDNAQGFLITEQFASQVYPELGFKVYPSEDEHGQFDGT
                     PVLHCEFEGDRIENVCTALKVIVPILTGLSAICAIPIIGWIICLIALLLSAAALAIVW
                     AASGEGDPHDAGLEDGDLEVGHDYILVRGDWCYDTGHTPGWNEIHPVKYVQKLNLRPE
                     LQPGVDHANPFDEGSDALTVDAFKNFLLDFCGETKKPFDPNVQIDQMEPPNRWTLHPV
                     VDGCEESDPVPIPK"
     gene            complement(4062..5087)
                     /locus_tag="VF240_10520"
     CDS             complement(4062..5087)
                     /locus_tag="VF240_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175682.1"
                     /translation="MNKCERQWRYICRTLLNVLKFLLYGWVFAWPRFLRLLCQLWLRR
                     CNRRKWRRGQSPIRCMRIPAKVYKRPDPLIYSQKYLKSLGLGVTYNNPDIELFENGLS
                     VNSHALKPDTTYEVRARIWNGSTDAPAVHMPVKFSYLNFGIGQKHVAIGETKVTVPVK
                     GAFGHPATAVVNWKTPAAPGHYCMLVNLIWGDDANPKNNEGQENTDVKKLNSPRATFE
                     FPLRNDTDRAGVFRLAADNYKPPPPPPCKDQPPAKTPEMTQDERNEKLREAIERHGAN
                     NFLVPPGWSVEIRPQEVALLPDAEQLITVVVTAPEDDFRGQETFNVHAFDDRNRLVGG
                     VTLTVEG"
     gene            5374..8328
                     /locus_tag="VF240_10525"
     CDS             5374..8328
                     /locus_tag="VF240_10525"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEX7175683.1"
                     /translation="MRHIKVFTFLLLLPLGAARAQQQNLARHVDPFVGTAAHGHTFPG
                     ATLPFGMVQLSPDTRLTGWDGCSGYHYSDNIVYGFSHTHLSGTGISDYGDILLMPTAG
                     PPRLHATDAAGRPEGGYASRFTHRRERASPGYYSVRLDDERILVELTATARTGLHKYT
                     FPRTAAANVTLDLAHRDKVLDSELRVVGPRSVVGFRRSEAWAKDQVVYFAAEFSRPFT
                     SYGIAAGGELKPETTEARGADVRAFFRFDARDGAPVLVRVGISAVSVEGARRNLEAEI
                     NHWDFERVRREADAAWEKELNKLQVRGGTRDQLRNFYTALYHAMLAPNLFTDTDGHYR
                     GRDGRIHRAEDFTNYTVFSLWDTFRAAHPLYAIIDRRRTLDFIRTFLAQYEQGGRLPV
                     WELAANETDTMIGYHAVSIIADAAAKGIDGFDLRKAFESMKHSARLKHFGLSSYAERG
                     FVSAEDEPESVSKTLEYAYDDWCVAQVARLSGRDDDYHEFIARAQSYKNLFDPQTGFM
                     RARRNADWAEPFDPREVNFNFTEANSWQYTFFAPHDVSGLMQMMGGREAFARKLDEMF
                     AADSRTTGREQADITGLVGQYAHGNEPSHHVAYLYNFAGQAWKTQRRVRQIMDEFYHP
                     TPDGLIGNEDCGQMSAWYIFSAAGFYPVTPGSPTYVIGSPLFEEVKFNLENGREFVVR
                     AEGVSAQNMYVQSATLDGKPHGNSYFTHADIMRGGRLVFRMGARPNKAWGAGYAEAPV
                     ARIDGGASVVPVPVVRAGGDAFRLRAEVRLQAASPELKIFYTTDGSEPDARSTPYRAP
                     FHIEQPTTIKAVAFDAGGARSRPAVARLQRIPNDWAVRVVSKYDPQYTAGGPVGLIDG
                     RRGGANFRTGAWQGHRGDFEAVIDLGRVTNVSKVGAGFLQDVRSWIWMPTRVEFDLSL
                     DGASYERAASVETDVPVRDESVMVKDFVREFARKRARYLRVRAYNYGVIPAWHPGAGD
                     RAWVFVDEIIVE"
     gene            complement(8451..>8682)
                     /locus_tag="VF240_10530"
     CDS             complement(8451..>8682)
                     /locus_tag="VF240_10530"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7175684.1"
                     /translation="IMGGTRYLRDLLRLFNGRVDLVLAGYNAGEGAVMKYGRRVPPYN
                     ETQNYVRIIGSRYAKGTGVQLTSKTSTPKGGK"
BASE COUNT         1471 a         2770 c         2931 g         1510 t
ORIGIN      
        1 cgtctatctg cccgtcttcc atgagggcgc gcttttctac tttggcgacg gccacgccct
       61 acagggcgac ggcgaggtct gcggctcggg gctggagacg agcatggacg ttgtcttcca
      121 cttcgagttg cggaagggca agcgcatccg ctggccgcgc atcgagaacg acgagtacat
      181 catggcgacg ggcagcgtcc ggcccctgat ggacgccttt cgcatcgcgc agatcgaact
      241 catcgactgg ctcgtcgccg actacggctt cgacaagtgg gaggcgcttc aggtcgtctc
      301 gcaagtgggc acgacgcgcg tggcgaatgc cgttgacccg aattacacgg tggtggccaa
      361 gtttccgaag aagtatttgc cgaggcccgg cgcgccttca aattgaggcc ggggttgact
      421 caactcaccg cagaggcgca gaggacgcag aggaagcgcg gaggcttgac gctttcactc
      481 gacggtgggt gtaagcgtca accctctgcg tagtctctgc gtcctctgcg cctctgcggt
      541 gagtcccact tcccctatgc tcaattaggg gccatcacgc tcgccgcgtc agggcaaccc
      601 cgcgccaaac actttgtcga agaagcgggc cgtcgccgtg ttcacgcgga gccagttggc
      661 gtgacgcatg aagtgatgcg tgtcgtcggg gatgattagc tcctcgaagg gcacgccggc
      721 gcgctcaagc ctgcgggcga gatcgaccgt ctggctgaag cggacgttgc ggtcgtcgtc
      781 gccgtggatg agcaacacgg gcgagcgcca cttcgagacg gaggcgacgg gcgaagaatt
      841 ccacgcgatg tctaaagcgg cctgcgtgtc gggcgctttc tcgtagcggc cttcgagcaa
      901 actttgcgct cgctcggccg tccagttgtg gacgccgtgg atgtccacgc cggcggcgaa
      961 caggtcggaa tcgcgcgcga gcgcgagcgc cgtgaggaag ccgccgtagg agccgccgta
     1021 gacgccgata cgtttgccgt caacctgtgg gagcgagcgc aggtactcgc ccgccgcctt
     1081 gatgtcctga tactcggccg cgccctgcgc gcccgcgctt gcgggccggt ggaagtcgta
     1141 gccgtagccg atgccgagcc ggtagtttat agagagcacg acgtagccgc ggctcgccag
     1201 atattgattg agggcgtagg cgttcgagta gtagtccgag tagtgccagc ccagaagcat
     1261 ctgccggggc gggccgccgt ggacgtagac gaccgccggc ttcttcgccg cgccgccgcc
     1321 gcccggccgc tcgaaaagct gcgcgtgtac ctgcacgccg tccggggcct tgtagacgac
     1381 cttacgcggc gtgacgagat gggcggcggg gaagtctgcg ctcacgcggt cttcggcgag
     1441 cacgcgaagg gggccgccgg cccaagggac gaccgccggg agcggcgggc gctgcggcgt
     1501 cgcgctgatg aggacgacgt ggcggccgtc gcccgtcacg acgggcgccc actcaagccc
     1561 gtcgccgggc gtcagcacct cgggcgcggc gcggtcaacg ctcacgcgca cgacgtgccg
     1621 gcggtcgatg tcgtcggccc cgcgtccggc gttgccggcg aagacgaggt ggcggcggtc
     1681 ggggctgatg gagacgtgct cggccatgta gtcgccgggc gtaagcagtg ttggctcgcc
     1741 gccgcgctcg gggatcgagt agaggtgcgg ccagccgtcc atgtaggaga gaaaaacgat
     1801 gcggccagcg gcggcccagt ggagattcac gccgccgtgc gtggagggcg gcgagccgcg
     1861 caggctgttc ggactcttcc aaagttggcg gccttcgccc gtcgcggcgt cggccgtcca
     1921 gatggcccag ccttgcgggc gctgttcgag aatggcctcg ggcgcgccgc cggcgccggg
     1981 gcgacggacg aaggccacgg agcggccgtc gggcgaccag cgcggtgagg agtcgcgcga
     2041 ggtcgagggc gcgagccaga gtatcggctg cgagtcgttg gtgtagacgc cgacgaaggc
     2101 gtggtcgccg cgtgatgaga tgaaggcgag gcgcgcggcg tcgggcgacc acacgggaga
     2161 acccggctct ccgcgcgcgc tgaacatctt cttcgcctgc gtcgagccgt ccgtgggtgc
     2221 gacccacagc ccacgatctt tcgcgaagac cacgcggtca cctttaggcg agacgacggg
     2281 ttcgtcaccg tcggcgagga gcttcggctc gccgcccgcg aaggcgacgc tccagatttg
     2341 caccttcgtc gcgacggggc ttgacgcggg gttgacgggc accgtgtcgt cccagttcga
     2401 cgagtgctcg ccgccgcgaa cgtagacgac gaagcggccg tccgaagaga cttgcagact
     2461 cgtgatttcc tgtccgtcgt ccgcgtcgta gttggtgaga cggcgcgggc gaaagtccgg
     2521 cccctcggcc acgtagacgt ttcgccggcc gcgctcgttg aaggcccacg cgacgcgcga
     2581 gccggcggcc gaggccgtca gctcgttcgg aaacggatag ccgcgcacct gttccatggt
     2641 gaacgactgc cccgcggcgc gggggacgaa ggccgcgagc aacaggatga agaggaacgg
     2701 tgcgcgacgg cgcgcgggtg cgaaggcggt gctcatgagt tcggctcctg gggctgtctg
     2761 taaagcggcg tcacgaatcc aaacacagcc gacgcgcaaa gcgcaagagc gaccggcgcg
     2821 gctgccgcta tttttagttg tgcgtgcggt aaggaagatt caccgcagag gcgcagagga
     2881 cttgggaagg caaaagtaaa aaggcaaaag gcaaaagtga gaaggcacat tgtctcttca
     2941 cttttgcctt tttccttcct ccgccctctg cgcctctatc cagacatgga attgagaacc
     3001 gctctcattg ttatttcggg atgggaaccg ggtctgactc ttcgcagccg tcaaccaccg
     3061 ggtgcagagt ccagcggttc ggcggctcca tctggtcgat ctgcacgttc gggtcgaagg
     3121 gcttcttggt ctcgccgcag aagtcgagca ggaaattctt gaaggcgtca acggtcaacg
     3181 cgtcgctgcc ctcgtcgaac ggattcgcgt ggtcgacgcc gggctgcaac tccggacgaa
     3241 ggtttaactt ctggacgtac ttgacggggt gaatctcgtt ccagccgggc gtgtggccgg
     3301 tgtcgtagca ccagtcgccg cggacgagga tgtagtcgtg gcccacctcc aggtcgccgt
     3361 cttcaagccc cgcgtcgtgc gggtcgcctt ccccgctcgc cgcccagact atcgccaacg
     3421 cggccgccga taacagcagc gcaatcaggc agatgatcca gccgatgatc gggatggcgc
     3481 agatggcgct caggccggtc aggatgggga cgatcacctt cagggccgtg cagacgttct
     3541 cgatgcggtc gccttcgaac tcgcagtgga ggacgggcgt cccgtcgaac tgcccgtgct
     3601 cgtcctcgga ggggtagacc ttgaaaccca gctcgggata cacctggctc gcgaactgct
     3661 ccgtgatgag aaagccctgg gcgttgtcgt tctgctgctg ggcgagggtg gcgctgggca
     3721 ggtgcgggga cagcaggatg ttgacgctgt aatcgttgtc gatgttttcc gggaacggct
     3781 tgttgaagcc gggcggctcc agcccgacca cgcggccgat ggcgcacatg tcgtgtccga
     3841 gacagatgag cttgccgccg aggaggtagt tgcagacgtc gaagatggcc atcatgccgc
     3901 cggcggccgc cagcgccgcc ccgacgagac cgcccccgaa gaacgaggct aggataatgc
     3961 cgagcacggc gaggatgcct ccggcaatcc cgacggccga gccgaggccg gtgtagtcct
     4021 gcgtagggac gcaatgtgtg tgttgtacgt atggcatcgc cttatccctc caccgtcagg
     4081 gtcacgccgc cgacgagccg gttccggtcg tcgaaggcgt ggacgttgaa tgtttcctgc
     4141 ccccggaagt cgtcctcggg cgcggtgacg acgaccgtga tgagctgctc ggcgtccggc
     4201 aggagcgcga cctcctgcgg gcgtatctcg accgaccagc cgggcgggac gaggaagttg
     4261 ttcgcgccgt ggcgctcgat ggcctcgcgc aacttctcgt tgcgctcgtc ctgggtcatc
     4321 tccggcgtct tggcgggcgg ctgatccttg cagggcggcg ggggcggcgg cttgtaattg
     4381 tcggccgcca gccgaaagac gcccgcgcgg tcggtgtcgt tgcgcagggg aaactcgaag
     4441 gtcgcgcgcg gcgagttgag cttcttcacg tcggtgttct cttgcccctc attgttcttg
     4501 gggttggcgt cgtcgcccca gatgaggttg acgagcatgc agtagtggcc cggcgcggcc
     4561 ggcgtcttcc agttcaccac ggccgtcgcc gggtgcccga acgcgccctt gacggggacg
     4621 gtgactttcg tctcgccgat ggcgacgtgc ttctggccga tgccgaaatt caggtaggag
     4681 aatttgacgg gcatgtggac tgccggcgcg tccgtcgagc cgttccagat gcgcgcgcgc
     4741 acctcgtagg tcgtgtcggg cttgagggcg tgcgagttga cgctcagccc gttctcgaac
     4801 aactcgatgt cggggttgtt gtaagtcacg ccgaggccga gcgacttgag gtacttctgc
     4861 gagtagatga gcgggtcggg tcgcttgtag accttcgccg gtatccgcat gcagcggatg
     4921 ggcgactggc cccggcgcca cttgcgccgg ttgcaacggc gcagccaaag ttgacagagc
     4981 agacgcagga agcgcggcca tgcgaagacc catccgtaga gcaaaaactt gaggacgttg
     5041 aggagcgtcc ggcagatgta gcgccattgc ctttcacatt tgttcatctt tgacctccgg
     5101 tgaacgagcg gatttgcgcg gcatagtgag aggtaataat ccgtcaagtc aaagggggat
     5161 gagtgtccgg cgagcgaggg ttctcgccgg cgggggagcg agcgaggttg agcgccagca
     5221 cattattccc gttgaaaacg gatgtcaata accggccttg accacgccgc gctttagttc
     5281 cattcgtcct acccgcaatt cgaatatcac tttagaatgt gctttcacat agaagcgtct
     5341 tcacgtcgcg acatccgagg tcagccgacc acgatgagac acatcaaagt cttcactttt
     5401 cttctgctcc tgccgctcgg cgccgctcgc gcccagcagc agaacctcgc gcgccatgtt
     5461 gatcccttcg tcggcacggc cgcgcacggc cacaccttcc ccggcgcgac cctcccgttc
     5521 ggcatggtgc aactcagccc cgacacgcgt ctcaccggct gggacggctg ctccggttat
     5581 cactactcgg acaacatcgt ctacggcttc agccacacgc acctgagcgg caccggcatc
     5641 tccgactacg gcgacatcct gctcatgccg accgccggcc ccccgcgcct ccacgcgacc
     5701 gacgccgccg gccgtcccga aggcggctac gcctcgcgct tcacacaccg ccgcgagcgc
     5761 gcctcgcccg gctattactc cgtccgtctc gacgacgagc gcatcctcgt cgagctgacg
     5821 gcgacggcgc gcacgggcct gcacaaatac accttcccgc gaaccgccgc cgccaacgtc
     5881 acactcgacc tcgcgcaccg cgacaaggtg ctcgactcgg agctgagagt cgtcggcccg
     5941 cgctcggtcg tcggcttccg caggtcggag gcgtgggcga aagatcaggt cgtctacttc
     6001 gccgccgagt tctcgcggcc gttcacctca tacggcatcg ccgcgggcgg cgagcttaag
     6061 ccagagacga ccgaggcgcg cggcgcggac gtgcgggcct tcttccgctt cgacgcgcgc
     6121 gacggcgcgc ccgtgctggt gcgcgtcggc atctccgccg tgagcgtcga gggcgcgcgc
     6181 cggaatctcg aagcggagat caatcactgg gacttcgagc gcgtgcgccg cgaggccgac
     6241 gccgcgtggg aaaaggagtt gaataagcta caggtacggg gcggcacgcg agatcagctc
     6301 aggaacttct acaccgcgct ctaccacgcg atgctcgcgc ccaacctgtt cactgacacg
     6361 gacggccact atcgcgggcg cgacgggaga atccaccgtg ccgaggattt caccaactac
     6421 accgtcttct cgctctggga caccttccgc gccgcgcacc cgctctacgc catcatcgac
     6481 cgccgccgca cgctcgactt catccgcacc ttcctcgcgc agtatgagca ggggggccgt
     6541 ctgcctgtgt gggaactggc cgccaatgag accgacacga tgatcggcta tcacgccgtc
     6601 tcgatcatcg ccgacgccgc cgcgaaaggc atcgacggct tcgaccttcg gaaagctttc
     6661 gagtcgatga agcacagcgc ccgtctcaag cacttcggcc tgtcttctta cgccgagcgc
     6721 ggtttcgtca gcgccgagga cgagccggag tccgtctcga agacgctcga atacgcctac
     6781 gacgactggt gcgtcgcgca ggtcgcgcgc ctcagtgggc gtgatgacga ctaccacgaa
     6841 tttatcgcgc gcgcgcagtc gtacaaaaac ctcttcgacc cgcagaccgg cttcatgcgc
     6901 gcccgccgga acgccgactg ggccgagccg ttcgacccgc gcgaggtcaa cttcaacttc
     6961 accgaggcga actcgtggca gtacaccttc ttcgccccgc acgacgtgag cggcctgatg
     7021 caaatgatgg gcgggcgcga ggcgttcgcg cgcaagctcg acgagatgtt cgccgccgac
     7081 tctcgcacca cgggccgcga gcaggccgac atcaccggcc tcgtcggcca gtacgcgcac
     7141 ggcaacgagc cgagccacca cgtcgcctat ctctacaatt tcgccggcca ggcgtggaag
     7201 acccaaaggc gcgtgcggca gatcatggac gagttttacc atccgacgcc cgacggcctc
     7261 atcggcaacg aagactgcgg gcagatgtcg gcgtggtata tcttcagcgc cgccggcttc
     7321 taccccgtca cgccggggtc gcccacctac gttatcggct ccccgctctt cgaggaggtc
     7381 aaattcaacc tcgaaaacgg ccgagagttc gtcgtccgcg ccgagggcgt ctcggcgcaa
     7441 aacatgtacg ttcagtccgc gaccctcgac ggcaagccac acggcaactc gtatttcaca
     7501 cacgccgaca tcatgcgcgg cggccgactc gtctttcgga tgggcgcgcg cccgaacaag
     7561 gcgtggggcg ctggctacgc ggaagcgccg gtcgcgcgga ttgacggtgg ggcgtcggtc
     7621 gtgcccgtcc ccgtagtccg cgccgggggc gacgcgtttc gcttgcgcgc cgaggtgagg
     7681 ctgcaagcgg cgtcgcccga gttgaaaatc ttctacacca ccgacggctc cgagccggac
     7741 gcgcgctcca cgccttaccg cgctccgttc cacatcgaac agccgacgac gatcaaagcc
     7801 gtcgccttcg acgccggagg cgcgcgaagt cgcccggcgg tcgcgcgtct ccaacgcatc
     7861 ccgaatgatt gggcggtgcg cgtcgtctcg aagtacgacc cgcagtacac ggcgggcggc
     7921 ccggtgggac ttatcgacgg gcggcgcggc ggcgcgaact tccgcacggg cgcgtggcag
     7981 ggccaccgtg gggacttcga ggcggtgatc gatctgggac gggtgacgaa cgtgtcgaag
     8041 gtcggcgcag ggtttttgca ggacgtgcgg tcgtggattt ggatgccgac cagagtcgag
     8101 ttcgatttgt cgctcgacgg cgcgagctac gagcgcgccg cctcggtcga gactgacgtg
     8161 cccgtgcgcg acgagtcggt gatggtgaaa gacttcgtgc gcgagttcgc gcgcaagcga
     8221 gcgcgctact tgagggtcag ggcttacaac tacggggtca tcccggcgtg gcatccgggg
     8281 gcgggagaca gggcttgggt gtttgtggat gagatcatcg ttgagtgatg ccgcgtcgga
     8341 caggtgaagt aaaaaggcaa aagtaaaaag gcaaaagtga ggaagcgtct cgcctcttca
     8401 cttttgcctt ttgccttttt acttttgcct taacttttgc cttaactgag ctacttgccg
     8461 cccttcggcg tcgaagtctt cgacgtgagc tgcacgcccg tgcccttggc atagcgcgag
     8521 ccgatgatgc gaacgtagtt ctgcgtctca ttgtagggcg ggacgcggcg gccatacttc
     8581 atcacggccc cctcgcccgc gttgtagccg gcgagcacga ggtcaacccg gccgttgaag
     8641 agccggagca ggtcgcgcag gtagcgcgtg ccgcccatga tg
//
LOCUS       DASVES010000262         3671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1240492, whole genome shotgun sequence.
ACCESSION   DASVES010000262 DASVES010000000
VERSION     DASVES010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240492"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VF240_10535"
     CDS             complement(<1..961)
                     /locus_tag="VF240_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7175685.1"
                     /translation="MRANINSYVEDFARRGEETAYASRRGLRQARWSYARVARAAYSF
                     ARELEARGVGRGERVLLWAANGPEWVAAFLGCSLAGVVVVPLDVESAPDFVARVGEQT
                     TPRLILLDAETAERGRGLADVPSLLLEGLEETTARHSAEAFAREVAPDDLLEIIFTSG
                     TTAEPRGVLLSHRNFLANLGPLEEEINRYIKWERPFHPIRFLNLVPLSHVFGQFMGIF
                     VPQLLGGEVHFQNSLNPSEMVETIRRERVSVVVAVPRMLESLREHVERHEAARGRGDA
                     FARALEGAKGAHPLRKWWTFRRVHRRFGLKFWAFVTGGATLPEE"
     gene            1156..1722
                     /gene="pyrR"
                     /locus_tag="VF240_10540"
     CDS             1156..1722
                     /gene="pyrR"
                     /locus_tag="VF240_10540"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX7175686.1"
                     /translation="MELVEKARIMDGPRINRALARLASEIVEENHGAGLLYLVGIQRR
                     GVPLANRLADKIADLEGERPPVGVLDITLYRDDLSTVGANPIVNRTDLPVDVDGKNVL
                     LIDDVLYTGRTIRAALDQLIDFGRPLRVQLAVLVDRGKEHRELPIQADFVGKFVPTKR
                     SEIIKVLLNEFDGEEGVAIVERPPAEGE"
     gene            1878..2921
                     /locus_tag="VF240_10545"
     CDS             1878..2921
                     /locus_tag="VF240_10545"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX7175687.1"
                     /translation="MLRNLLGIRDLSAEEITHILDTAETFRGVSQREIKKVPALRGRT
                     VINLFFEPSTRTRTSFEIAGKRLSADVINISASSSSVSKGETLLDTARNLEAMAPDLI
                     VIRHPSSGAPQQLARVCRAGIINAGDGAHEHPTQALLDALTIRQQKGRIDGLKVAILG
                     DILHSRVARSNAHLLTKLGARVSVAGPGTLAPPAFASLAGDGSLLRVERRMEDAIEDA
                     DVVVVLRIQRERQESAFFPSLREYAVHYGLNLKRLSLAKPDAIVMHPGPMNRGIEIAS
                     DVADGSRSLILDQVSNGVAVRMAVLYLLAGSTATVADAAPPQIEPTKPQTAAPEEPLQ
                     RAALSAVPTERAE"
     gene            2950..>3671
                     /locus_tag="VF240_10550"
     CDS             2950..>3671
                     /locus_tag="VF240_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7175688.1"
                     /translation="MRLLIANGYVIDPTQGVNAGRDLFIEDGRVVGLLGRGESRPEGA
                     EVFDATGLVIAPGFIDMHVHLREPGQEYKETVASGAAAAVAGGFTSVCAMPNTDPVND
                     NPAVTAFIIEQAQAAGFANVFPVGAITKGSRGEELAEMGEMKRAGIVAVTDDGRPVPT
                     PGMMRRAMEYARGFDLPVIDHCEDKSLSAGGVMHEGDWSLRLGLRGMPALAEESDVAR
                     DCALAELTLARLHIAHVSTRGA"
BASE COUNT          648 a         1286 c         1156 g          581 t
ORIGIN      
        1 tctcctcggg aagcgtcgcg ccgcccgtca cgaacgccca gaacttcaac ccgaagcggc
       61 gatggacacg ccggaacgtc caccacttcc gcaagggatg cgcgcccttc gcgccttcga
      121 gcgccctcgc gaaagcgtca ccacggccgc gcgcggcctc gtgtcgctcc acgtgctcgc
      181 gtagcgattc aagcattcgc ggcacggcga cgactacaga cacgcgctcg cgtcgaatgg
      241 tctcaaccat ctcggacggg ttgagcgagt tctgaaagtg aacctcgccg cccaatagtt
      301 gcggcacgaa gatgcccatg aactgcccga agacgtggct cagcggcacg aggttcagaa
      361 acctgatggg atggaacggg cgctcccatt tgatgtaacg attgatctcc tcttcgagcg
      421 gcccgaggtt ggcgagaaag tttcggtgcg acaacaacac accgcgcggc tcggccgtcg
      481 tccccgacgt gaagatgatc tccagcagat cgtcgggcgc aacctcccgc gcgaaagctt
      541 cggcgctatg tcgcgccgtc gtctcctcca gcccttcgag caacagggag ggaacgtccg
      601 cgaggccgcg cccgcgctcg gccgtctcgg cgtcgagcaa gataagacgc ggggtggtct
      661 gttcgccgac gcgcgcaacg aagtccggcg cgctctccac atctagcggc acgaccacca
      721 cgccggcgag cgagcagccg aggaaggccg cgacccactc cgggccatta gccgcccaca
      781 gcaacacgcg ctcgccccga cccacgcccc gcgcctcaag ctctcgcgcg aagctataag
      841 cggcccgcgc cacacgcgcg tagctccaac gcgcctgccg cagcccgcgc cttgaagcat
      901 aagccgtctc ctcgccccgc cgcgcgaaat cctccacata gctgttgatg tttgcacgca
      961 tcgagtccac gacgattgtg caactcgcct gccacgttgt cgagccaacc ctgcgggcac
     1021 ttgggccggc gcgggctgac aagtcgctcc gtcatttgga tgatattaac tgcgtcgggc
     1081 gtgcgcggcc ggcgcgagga attcaggggc agaaacttcc atcctctcac ggccctgtgc
     1141 tagtatcccc ggctaatgga actggtcgag aaagcacgca tcatggacgg gccgcggatc
     1201 aaccgggcgc tggcgcggct ggcgtcggag atcgtggagg agaaccacgg ggcggggctc
     1261 ctgtacctcg tcggcattca gcggcggggc gtgccgctgg ctaaccgctt ggccgataag
     1321 atcgccgacc tcgaaggcga gcgcccgccc gtcggcgtgc tcgacatcac gctttatcgc
     1381 gatgacctct cgaccgtcgg cgccaacccc atcgtcaatc gcaccgatct gcccgtggac
     1441 gtggacggca agaacgtcct gctcatcgac gacgtgctct acaccggccg caccatccgt
     1501 gccgcgctcg accagttgat cgacttcggg cggcccctgc gcgtgcagct cgccgtcctg
     1561 gttgaccgcg gcaaggagca ccgcgagctt ccgatacagg ccgacttcgt cggcaagttc
     1621 gtgccgacga agcggagtga gatcatcaaa gttctcctca acgagttcga cggggaggag
     1681 ggggtggcca tcgtcgaacg gccccccgcg gaaggggagt gagacgggcg gctccgacgg
     1741 ggccgccttt ttttatcaga aaattcattt agtgatgagt gataagtgat gagtgatgag
     1801 aaaagacaga agacggatgc cttttcttat cacttatcac tcatcactca tcacctgttc
     1861 tgttggagaa tctggtagtg ctcagaaacc tgctcgggat acgcgacctg tcggccgagg
     1921 agatcacgca catcctcgac acggcggaga ctttccgcgg cgtctcccaa cgcgagatca
     1981 agaaggtgcc cgcgctgcgc ggacgcaccg tcatcaacct cttcttcgag ccttcgacgc
     2041 ggacgcgcac ctccttcgag atcgccggta aacgcctgtc ggccgatgtc atcaacatca
     2101 gcgcgtcttc gtcgagcgtg tcgaagggcg agacgctgct cgacacggcg cgcaaccttg
     2161 aggcgatggc ccccgacctc atcgtcatcc gccacccctc ctcgggcgcg ccgcagcagc
     2221 tcgcccgcgt ctgtcgcgcc ggcatcatca acgccggcga cggcgcgcac gagcacccca
     2281 cacaggcgct cctcgacgcg ctcaccatcc gccagcagaa ggggcgcatc gacggcctca
     2341 aggtcgccat cctcggcgac atcctccact cgcgtgtcgc ccgctccaac gcgcacctgc
     2401 tcaccaagct cggcgcgcgt gtctccgtgg cggggccggg cacgctcgcg ccgcccgcat
     2461 tcgcctcgct tgcgggcgat gggagtctgc tgcgcgtcga gcggcggatg gaagatgcca
     2521 tcgaagacgc ggacgtcgtt gtggtcttac gcatccagcg cgagcggcag gagtcggcct
     2581 tcttcccctc cttgcgcgag tacgccgtcc actacggact gaatttgaag cgcctgtcgc
     2641 tggcgaaacc cgacgccatc gtcatgcatc cggggccgat gaaccgcggc atcgagatcg
     2701 cttcggacgt ggccgacggc tcgcgctcgc tcatcctcga tcaggtctcg aacggcgtcg
     2761 ccgtccgcat ggccgtcctc tacctcctcg ccgggagcac ggcgacggtc gccgacgccg
     2821 cgccgccgca aatcgaaccg accaagcctc aaacggccgc gcccgaagag ccgctccaga
     2881 gggccgcctt gagcgccgtg ccgacggaga gggccgagtg accggcggcg tttgattcat
     2941 acgtaaacga tgagactgct aatcgctaac ggatacgtga tcgacccgac gcagggcgtc
     3001 aacgccggcc gcgacctgtt catcgaagac ggccgcgtcg tcggactgct cggccgcggc
     3061 gagtcgcggc ccgagggcgc cgaagttttc gacgccacgg gactcgtcat cgcccccggc
     3121 ttcatcgaca tgcacgtcca cctgcgcgag ccggggcagg agtacaagga gacggtcgcg
     3181 tcgggcgcgg cggccgccgt cgcgggcggc ttcacgagcg tttgcgcgat gccgaacacc
     3241 gaccccgtca acgacaaccc cgccgtcacc gccttcatca tcgagcaggc gcaggccgcc
     3301 gggtttgcca acgtcttccc cgtgggcgcg atcacgaagg ggtcgcgcgg cgaagagctg
     3361 gccgagatgg gcgagatgaa gcgcgccggc atcgtcgccg tcacggacga cggacggccc
     3421 gtccccacgc ccggcatgat gcgccgcgcg atggagtacg cgcgcggctt cgacctgccc
     3481 gtcatcgacc actgcgagga caagtcgctt tcggccggcg gcgtgatgca cgagggcgac
     3541 tggtctctgc gtctcggcct gcgcgggatg ccggcgctcg ccgaagagtc ggacgtggcg
     3601 cgcgactgtg cgctcgccga gttgacgctc gcgcgactcc acatcgcaca cgtctcgacg
     3661 cgcggggcca t
//
LOCUS       DASVES010000263         9124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1394602, whole genome shotgun sequence.
ACCESSION   DASVES010000263 DASVES010000000
VERSION     DASVES010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394602"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VF240_10555"
     CDS             complement(<1..992)
                     /locus_tag="VF240_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175689.1"
                     /translation="MTKWRETLSRLRRAPSRAGATLLLLPALVSGALIICSATAPAQK
                     KKAAKGAKTTAAATPTAAPKKTADKSLTVKTEPKAVVWLDEVRHGVTDDAGSLAVKNV
                     SAGRHTLRVRARGFSERTLALLPTQRGTIEVRLTPTTDEAELLFQQAEELSAKGAEGR
                     TQAKELYRRALKLRPRYPAAHVGLARALAADDDHNAALEALAAARRLRPSYPEASAVE
                     GRILRELAEYDESIAAFERAVREGRGFQPEAHAGLGLVFTDKGDHEAAVEHFRKAVAQ
                     LSDTEPALYQLLGAAYEKLEKWKEAVAAYEKYLELAPEGKLAPAIRSIIDQLRQ"
     gene            complement(1021..3357)
                     /locus_tag="VF240_10560"
     CDS             complement(1021..3357)
                     /locus_tag="VF240_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7175690.1"
                     /translation="MKQRAHLSQRATFLLASFALLAAAAALPLASASCATQRSAAQDG
                     PEAAVRLRSLTRGAGLPAESVVESIERDYAGTRTGALARVLRARVRAAAGDFTGAAAL
                     LDSRDVRERTRINDHALFMRAGWLEQAGRRVEARAAFEQLARDFPDSPRARAATLRAA
                     ALILQDSQPSAVPVTLKKLADADDADALLLIAKAYEQSNDPTRALGAYRRLYFYAPVS
                     EDTEREAAAGIARLNSTTAPASAEEAATRADKLFAAGRHVAAANAYADAFARFPDRAT
                     PESRIRAGQAAINAKRTPEAVSFLSGALSAPEELRAEAQYNLAKAHARAKSWPSLRAG
                     LEEMRRAHPKSPWTMRALTESGAAARDAKNTADAFDFFRAAVASFPGAPETAGAQFEL
                     AWAAHESKNFAESSRLLIEHLADYAGRNTDNRGKAGYWAARDAERAGRTAEARAIYEA
                     MLDRYEANWYGHLSRQRLDQMTRNSGGQARATNFAPDSPVARAVANLKYVNVAEEHAG
                     PAEDSARARAEELDLAGLSDLAHAELDAALRSAPASPRLSLAKARVHRSRGENLEAFR
                     VLARSFPDYSQMEVSELTADEWDIFYPLVHWDAIVQESRARNLDPYTVAGLIRQESVF
                     NPRVSSHADAHGLMQLIPGTARMMARRVGSERAISVEALYDAPFNIKLGTAYLREQLD
                     RFGRIEYVAAAYNAGPGRAVAWRASLPAEMDEWTEAVPFRETRGYVQGVVRNGLQYRR
                     LYDEQGRFRPEVGARAVSAGQAGDTVRARRVANDEEEE"
     gene            complement(3598..5067)
                     /locus_tag="VF240_10565"
     CDS             complement(3598..5067)
                     /locus_tag="VF240_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7175691.1"
                     /translation="MPSRDLEQSLREDIEGYVESRLSGVREEFARLRDQFNQAFERLS
                     ERLSADAQSDAPVAVAIADHLRQARNAGIETAAEGSSRARSSSDVAIIKAAVEDIQGQ
                     QSQGEILNTLVNRAASFAPRVAFFVVKNERITGWRARGMEGTVGDDSVRELSMPLTSD
                     TLLAEAARSRQSWSGAPGAHAEDNRLYGHFGGEPPARLIAVPLVVRDKTVAVLYADTA
                     EQDADAVNLEALETLVRVSGMAVDLVAARRGGGSESGSAVAAAPQRERESESPAQPER
                     VEEVSAEPSFTEAAATAPTVEAEPVETREPEPFVEAEPAPADDEEEARPEYVAEPDYE
                     PAPAQQQQQQEGQAPDPWTGRGDIHSAPAFEPAATAPVAASPQAESSSMRRRAGADLP
                     IEVSEEERPVHDRARRFARLLVSEIKLYNEQKVREGRDAGDIYGRLRDDIDRSREMYD
                     KRFAEQVGGRYDYFHHELVSTLAEGDDAKLGSGYPGAAA"
     gene            5368..5820
                     /locus_tag="VF240_10570"
     CDS             5368..5820
                     /locus_tag="VF240_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175692.1"
                     /translation="MSPTILIADDYDDNRELLRLMLAVEGYRVREARDGRECVESARA
                     ETPDLALIDLSMPRLDGWGALEEMRADPRTRSIPCVAVTAFAAEQDRRRALAAGFDDY
                     ISKPFRARDLLELVARLLERGRDAERPDAANGKGAAHDAPAEHGGKRS"
     gene            5832..6104
                     /locus_tag="VF240_10575"
     CDS             5832..6104
                     /locus_tag="VF240_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175693.1"
                     /translation="MTEKKDEELTPGRVLVAFEKLFGGGRGVRRFSRSGLRYVELGGE
                     AVLVEQNPKKGSEWAALAREGRRVAWVLRDGEYLARVVDDEVTMLD"
     gene            6451..7677
                     /locus_tag="VF240_10580"
     CDS             6451..7677
                     /locus_tag="VF240_10580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX7175694.1"
                     /translation="MRKLFTFFALAVLCAAVLAVTPETVVTRGQDESAANKQAAKGKL
                     LRKGDKKIANQYIVVFNEWATGPEGEFSQSAQAAEQLTLAFGGKIKHVYKHALNGYAA
                     EMSEAEALAISDDPRVAYVEEDGEMSLVATQSNPPWGLDRIDQRNLPLSGAYTYNWTG
                     SGVRAYVIDTGIRTTHTQFGGRASAVYDVNGGNGQDCNGHGTHVAGTIGGSTYGVAKS
                     AMLRGVRVFGCGGTTSTSNIIAGVNWVTANHIKPAVANMSVGGPASSSMDTAVNNLIN
                     AGVTVAVAAGNSNGANACNYSPARAAAAITVGSTTSTDARSSFSNIGACLDLFAPGSS
                     IQSAWYTSDTATATLNGTSMASPHVAGVAALYKQAVPSASPATIRNAIVNNATTGKVT
                     NAGTGSPNRLLYSLFF"
     gene            8131..9048
                     /locus_tag="VF240_10585"
     CDS             8131..9048
                     /locus_tag="VF240_10585"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEX7175695.1"
                     /translation="MKVIDQFVSNLYVGEPQEFEQLRVAPVFVREDRPFPYLDLEEAL
                     KRQVVEVTEVSEQGSVPSLRVTNTGDLDVIVLDGEELIGAKQNRIVNTTIIVPARSTV
                     EIPVSCVEARRWGYTSRSFATSANPSYSSLRARKHSSVTASLRETGTHTSNQSEIWGD
                     IAGKSSRLRSYSPTSSMSDMFDTTISPEADRQIVEQIPHSEGQVGYLAFVRGGFAGGD
                     VFGSAELCRGKLRKFVRGHYLDALDEGVRFPAITVEEVIAQVRAAQQEQFATVGKGSE
                     VRFEARDVQGTWKLVDDLIPHLLVLPKLN"
BASE COUNT         1361 a         3044 c         3213 g         1506 t
ORIGIN      
        1 tgctggcgca gttggtcgat gatggagcgg atggcggggg cgagtttgcc ttcgggggca
       61 agctccagat acttctcgta ggcggcgacg gcctctttcc acttttcgag tttttcgtag
      121 gcggcgccga gcagttggta gagcgccggc tcggtgtcgg agagctgcgc gacggccttg
      181 cggaagtgtt cgaccgctgc ctcgtggtcg cctttgtccg tgaagaccaa ccccaacccc
      241 gcgtgggcct cgggctggaa gccgcgcccc tcgcggacgg cgcgctcgaa ggcggcgatt
      301 gactcgtcgt actcggccag ctcgcgcagg atgcgcccct cgacggccga cgcctcgggg
      361 tacgaggggc ggaggcggcg cgcggcggcg agtgcttcaa gcgcggcatt gtggtcgtcg
      421 tcggccgcga gagcgcgcgc gaggccgacg tgggcggccg ggtatctcgg acgcagcttg
      481 agcgcgcggc ggtaaagctc tttggcctgt gtgcgcccct cggcgccttt cgcggaaagt
      541 tcttcggcct gctggaagag cagctcggcc tcgtcggtcg tcggggtgag tcgcacctcg
      601 attgtgccgc gctgcgtggg caggagcgcg agcgtgcgct cactgaagcc gcgcgcgcgg
      661 acacgcagcg tgtgccggcc cgccgagacg ttcttgacgg cgagcgagcc ggcgtcgtcg
      721 gtgacgccgt ggcgcacctc gtcgagccag acgacggcct tcggctcggt cttgacggtg
      781 agcgacttgt cggcggtctt tttcggcgcg gccgtcggtg ttgccgccgc cgtggtctta
      841 gcgcccttcg cggccttctt tttttgcgcc ggcgcggtcg cggaacagat gatgagggcg
      901 ccgctgacga gcgcgggcag tagtagtaac gtcgcgcccg cgcgggacgg cgcgcgccga
      961 agtcgcgaca gtgtctctct ccatttcgtc atactcacgc ttctctccac ggcgtcacac
     1021 ttattcctct tcttcgtcgt tcgcgacgcg gcgcgcgcgc accgtgtcgc cggcctgacc
     1081 ggccgagacg gcgcgcgcgc cgacctcggg gcggaagcgt ccctgctcgt cgtagaggcg
     1141 tcggtactgc aagccgtttc gcaccacgcc ctgcacgtag ccgcgtgtct cgcggaaggg
     1201 gacggcctcg gtccactcgt ccatctcggc gggcagcgag gcgcgccacg ccacggcgcg
     1261 acccggcccc gcgttgtagg cggcggcgac atactcgatg cggccgaagc ggtcgagctg
     1321 ttcgcgcaga taggcggtgc cgagcttgat gttgaatggc gcgtcgtaga gcgcctcgac
     1381 gctgatggcg cgctcagagc cgacgcgtcg cgccatcatc cgcgccgtgc cggggatcaa
     1441 ctgcatcagc ccgtgcgcgt cggcgtgcga ggagacgcgc gggttgaaga ccgactcctg
     1501 ccggatgagg ccggcgacgg tgtaggggtc gaggttgcgc gcgcgcgact cttgcacaat
     1561 ggcgtcccag tggacgagcg ggtagaagat gtcccactca tcggccgtca gctccgagac
     1621 ctccatctgc gagtagtcgg ggaaactgcg cgcgaggacg cggaaggctt cgagattttc
     1681 gccgcgcgag cgatgaactc gcgccttcgc cagactcagg cgcgggctgg ccggggcgga
     1741 gcgcagcgcc gcgtcgagtt cggcgtgcgc gaggtcggag agaccggcga ggtcgagttc
     1801 ttcggcgcgt gcgcgggccg agtcttcggc ggggccggcg tgctcctcgg cgacgttgac
     1861 gtacttgagg ttggcgacgg cgcgcgcgac gggtgagtcg ggggcgaagt tggtcgcccg
     1921 cgcctgcccg ccgctattgc gcgtcatctg gtcgaggcgt tggcgtgaga gatggccgta
     1981 ccagttggcc tcgtagcggt cgagcatcgc ctcgtagatg gcgcgcgctt cggcggtgcg
     2041 gccggcgcgt tcggcgtcgc gcgccgccca gtagccggcc ttgccgcggt tgtcggtgtt
     2101 gcggccggcg tagtcggcga ggtgttcgat caggaggcgg gacgactcgg cgaagttctt
     2161 cgactcgtgc gcggcccacg ccagctcgaa ctgcgcgccg gccgtctcgg gcgcgccggg
     2221 gaaggaggcg accgcggcgc ggaagaagtc gaaggcgtcg gcggtgttct tcgcgtcgcg
     2281 ggccgccgcg cccgactcgg tgagcgcgcg catcgtccac ggactcttcg ggtgtgcgcg
     2341 gcgcatctcc tccagacccg cgcgcagcga cggccagctc ttcgcgcgtg cgtgcgcttt
     2401 cgccaagttg tactgcgcct cggcgcgcag ttcttcggga gcagacagag cgccgctcag
     2461 gaaactgacc gcctccggcg tccgcttcgc attgatggcc gcctgaccgg cgcggatgcg
     2521 cgattcgggt gtggccctgt cagggaagcg ggcgaaggcg tcagcgtaag cgttggcggc
     2581 ggcgacgtgg cggcccgcgg cgaagagttt gtcggcgcgc gtcgcggcct cttcggcgga
     2641 ggccggcgcg gtcgtcgagt tcaggcgggc gatgccggcc gcggcttcgc gctcggtgtc
     2701 ttcagagacg ggcgcgtaga agtatagacg gcggtaagcg ccgagcgcgc gcgtggggtc
     2761 gttcgactgt tcgtaggctt tcgcgataag gaggagcgcg tcggcgtcgt ccgcgtcggc
     2821 gagttttttg agggtgacgg ggacggccga cggctggctg tcctgaagga tgagcgcggc
     2881 ggcgcgcagg gttgcggcgc gcgcacgcgg ggagtcgggg aagtcgcgcg ccagttgctc
     2941 gaaggcggcg cgcgcctcga cgcgccggcc ggcctgctcc agccaaccgg cgcgcatgaa
     3001 gagggcgtgg tcattgatgc gcgtgcgctc gcgcacgtcg cgcgagtcga gcagggcggc
     3061 ggccccggtg aagtcgcccg ccgcggcgcg gacgcgtgcg cgcagcacgc gggccagcgc
     3121 gccggtgcgc gtccccgcgt agtcgcgctc gatggattcg acgaccgact cggcgggcag
     3181 ccccgcgccg cgcgtcagcg agcgcaggcg cacggccgct tcggggccgt cctgcgcggc
     3241 ggagcgctgc gtggcgcaac tcgcgctcgc gagcggcagc gcggccgccg cggcgagcag
     3301 ggcgaagctc gccagcagga acgtggcgcg ctgggagaga tgagcgcgtt gcttcaaaga
     3361 atttcggttc aattcgtcac ccggtcttcc ctggattcgg ttcacgttgg agagtatgca
     3421 agtcttcgga ggtcgcgcgc aaacgcgcag ccgcgatgat tcagagggtg agaatttgat
     3481 gcggcctccg gcgaaggcgg ttcgccgccg tgtgatttca gacgagaaga gcggcgcgcg
     3541 gggaggtgcc cccgcgcgcc gcttgggtag ttgtggcgtt gtcgtcgttc cgattcgtca
     3601 ggccgccgcg ccggggtagc cggagccgag cttggcgtcg tcgccttcgg cgagcgtgct
     3661 gacgagttcg tggtggaagt agtcgtagcg cccgccgacc tgctcggcga aacgcttgtc
     3721 gtacatctcg cgcgagcggt cgatgtcgtc gcgcaggcgt ccgtagatgt cgccggcgtc
     3781 gcggccctcg cgcaccttct gctcgttgta gagcttgatc tcggagacga gcaggcgcgc
     3841 gaagcgtcgg gcgcggtcgt gtacggggcg ctcctcttcg ctcacctcga tgggcaggtc
     3901 ggcgccggcg cgccggcgca tggacgatga ttccgcctgt ggcgaggccg cgacgggggc
     3961 ggtggcggcc ggctcgaagg cgggcgcact gtggatgtcg ccgcgtccag tccacgggtc
     4021 aggcgcttgc ccctcctgct gctgctgctg ctgcgccggc gccggctcgt agtcgggttc
     4081 cgccacgtac tcgggccggg cctcttcttc gtcgtcggcc ggcgcgggtt cggcttcgac
     4141 gaacggctcc ggctcgcgcg tttccaccgg ctcggcctcg acggtgggcg ccgtggcggc
     4201 cgcctcggtg aacgaaggct ccgcgctgac ttcttcgacg cgctcgggct gtgccggcga
     4261 ctcggactcg cgctcgcgtt gcggggcggc cgccacggcc gaaccggact cactgccgcc
     4321 gccgcgtcgc gccgcgacca gatcgacggc catgccggag acgcggacga gtgtctccag
     4381 agcttcgagg ttgacggcgt cggcgtcctg ctcggccgtg tcggcgtaga gcacggccac
     4441 ggtcttatcg cgcacgacga gcgggacggc gatcagacgc gcgggcggct cgccgccgaa
     4501 gtgtccgtag aggcggttgt cttcggcgtg cgcgccgggc gcgccgctcc agctctgacg
     4561 cgagcgcgcg gcctcggcga gcaaggtgtc ggaggtgagc ggcatcgaga gttcgcgcac
     4621 cgagtcgtcg cccacggtgc cttccatgcc gcgcgcgcgc cagcccgtga tgcgctcgtt
     4681 cttgacgacg aagaaggcga cgcgcggcgc gaaactcgcc gcacggttga cgagggtgtt
     4741 cagaatctcg ccctgcgact gctggccctg gatgtcctcg accgccgcct tgataatggc
     4801 cacgtcgctc gaagagcgcg cgcgcgagga gccttcggcc gccgtctcga tgccggcgtt
     4861 gcgcgcctgt cgcaagtggt cggcgatggc gacggcgacg ggggcgtccg actgcgcgtc
     4921 ggccgacagc cgctcggaga ggcgctcgaa cgcctggttg aattgatcgc ggagacgcgc
     4981 gaactcctcg cgcaccccgc tcagtcggct ctcgacgtag ccctcgatgt cttcgcgcag
     5041 gctctgttcc aggtctcggc taggcaactt tttggtctcc ttcgtcggat gttcggacgg
     5101 atggccgcat gggggcggcg cgtaactgcc ggggggtggc ttcgtgttct cgctggtgtc
     5161 ggcggcgacg cgctgcgcgc gcccgcgtgg ggccgggcga gtagtttcgg cccgtggcct
     5221 ctgcgattat ggtttcggcg cgcgagcagt gtcaagaacg agctttcccg gtttgcgatt
     5281 tcccagccgg ctttctatga ttagccctcc cccgtgggcc gcgtgtccga aacgcgcgcc
     5341 ccgcgcgaaa catcttcgac tcggaacatg tcccccacca ttctcatcgc cgacgactac
     5401 gacgacaatc gcgaactgct gcgcctgatg ctcgcggtcg aaggctatcg cgtccgcgag
     5461 gccagagacg ggcgggagtg cgtcgagtcg gcgcgcgccg agacgcccga cctcgcgctc
     5521 atcgatctgt cgatgccgcg gctcgacggc tggggcgcgc tcgaagagat gcgcgccgac
     5581 ccgcggacgc gctcgatccc ctgcgtcgcc gtcacggcct tcgccgccga gcaggatcgg
     5641 cggcgcgcgc tcgccgccgg cttcgacgac tacatctcca agcccttccg cgccagagac
     5701 ctgctcgaac tcgtcgcgcg cctgctcgaa cggggccgtg acgccgagcg gcccgacgcg
     5761 gccaacggta aaggcgcggc ccacgacgcg ccggcggagc acggcggaaa aaggtcctga
     5821 cggaaaaagc cttgacggaa aaaaaagatg aagagctgac gccgggccgc gtcctcgtcg
     5881 ccttcgagaa attgttcggc ggcgggcgcg gcgtgcgccg cttcagccgc tccggcttgc
     5941 gctacgtcga actcggcggc gaggccgtgc tcgtcgaaca gaacccgaag aaggggagcg
     6001 agtgggccgc gctcgcacgc gaggggcgtc gcgtcgcctg ggttctgcgc gacggcgagt
     6061 atttggcgcg cgtcgtcgat gacgaagtga cgatgctcga ctgagtcacg gtgcgcgacg
     6121 gatgcgcggt cagggtcgcc cacaaaattt ttcttgcaac gcgccgcaac cctgttgtag
     6181 agtgcgcccc tctacgtcgt caaaaaaaac agcgcgggcc tcaaccccca aagctcacgc
     6241 tgcttccccc agactccggc gtagggccac tactcccgaa cgttccttgt ccctgcattc
     6301 atagagaagc actgttgcct cattcgacgg tgatggtttg acgtgtccac agggccgccg
     6361 cgtccgccgg cggccatccg aagcgggggg cgcccacgag tttagaaccg ccttcccgtt
     6421 actctcacga tagacttctc tggaggggaa atgagaaaac tgttcacgtt cttcgcgctc
     6481 gcagttctgt gcgccgccgt gctggcggtg acccccgaga cagttgttac acgcggccag
     6541 gacgagtcgg ccgctaacaa gcaggcggcc aaaggtaagt tgctccgcaa gggcgacaag
     6601 aagatcgcta accagtacat cgtcgtcttc aacgagtggg cgaccggccc cgagggcgag
     6661 ttctcgcaat ccgcacaggc cgccgagcag ttgaccctcg ccttcggcgg caaaatcaaa
     6721 cacgtctaca agcacgcctt gaacggctac gccgccgaga tgtcagaggc ggaggctctc
     6781 gccatctccg acgacccgcg cgtcgcctac gtcgaggagg acggcgagat gtctctggtg
     6841 gccacacagt cgaacccgcc gtggggcctc gaccgcatcg accagcgcaa cctcccgctc
     6901 tccggcgcct acacctacaa ctggacgggg tcaggcgtgc gcgcctacgt catcgacacc
     6961 ggcatccgca ccacacacac gcagttcggc gggcgggcct cggccgtcta cgacgtgaac
     7021 ggcggcaacg ggcaggactg caacggccac ggcacgcacg tcgccggcac catcggcggc
     7081 tcgacctacg gcgtcgccaa gagcgcgatg ctgcggggcg tgcgcgtctt cggctgcggc
     7141 ggcacgacct cgacctcgaa catcatcgcc ggcgtcaact gggtgacggc caaccacatc
     7201 aaaccggccg tggccaacat gagcgtcggc ggcccggcct cctcgtcgat ggacacggcc
     7261 gtcaacaacc tcatcaacgc gggcgtcacc gtggccgtcg ccgccggcaa cagcaacggc
     7321 gccaacgcct gcaactactc gccggcgcgc gcggcggccg ccatcactgt cggctcgacg
     7381 acttccacgg acgcgcgctc gtcgttctcc aacatcggcg cctgtctcga cctgttcgcg
     7441 cccggctcct cgatacagtc ggcttggtac acgagcgaca cggcgacggc caccctcaac
     7501 ggcacatcga tggcctcgcc gcacgtcgcg ggcgtggccg cgctctacaa gcaggccgtc
     7561 ccgtcggcct cgcccgcgac catccgcaac gccatcgtca acaacgcgac gacgggcaag
     7621 gtgacgaacg ccggcaccgg ctcgccgaac cggctgctct actcactgtt cttctgaggc
     7681 gcggcgactg gccacttact tgagccgggg gcgactagga ctcaccgcag aggcgcagag
     7741 gacgcagaga tgacgcagag ggttgagcat tcactcgacg ttgaatgagt gcgtcaagcc
     7801 tgcggcgtcg tctctgcgtc ctctgcgcct ccgcggtgaa cttccgtgcc tgatgctcaa
     7861 gccgcgagcc atcattcatc gcgcctcgcg acccgaaata ctttcaagca accacccccg
     7921 tgtctgctac aatccaagcc atacgcccga cacctcgcaa cggctcggac aggcttagac
     7981 ggtgttgcca gacgctcccg cgaacaacgc ctaatcccgc ccacattcaa acttaaacgg
     8041 aaggggaagt gctttcacta ccttcccgcg cggtgcctta tggcacgcga aacgcggatc
     8101 acagaaagcg cctccccgca ggaggtgtgc atgaaagtaa tcgatcaatt cgtcagcaat
     8161 ctttacgtcg gggagccgca ggagtttgag caactgcgcg tcgcgccggt cttcgtccgt
     8221 gaggacaggc ccttccccta tctcgatctc gaagaggcgc tcaagcgcca ggtcgtcgag
     8281 gtcaccgaag tctccgagca ggggagcgtg ccctctctgc gcgtcacgaa cacgggcgac
     8341 cttgatgtca tcgtcttaga cggcgaggaa ctcatcggcg ccaagcagaa ccgcatcgtc
     8401 aacacgacga tcatcgtccc ggcgcgctcc accgtggaga ttcccgtctc atgcgtcgag
     8461 gcgcgccgct ggggctacac ctcgcgcagc ttcgcgacga gcgcgaaccc gtcctactca
     8521 tcgctccgcg cgcgcaagca cagctccgtc acggcctccc tgcgcgagac gggcacgcac
     8581 acgtccaacc agtcggaaat ctggggcgac atcgccggga agtcttcgcg cctgcgctcc
     8641 tacagcccga cctcctcgat gtcggacatg ttcgacacga cgatcagccc ggaggccgac
     8701 cggcagatcg tggagcagat tcctcacagc gaggggcagg tgggttatct ggcgttcgtg
     8761 cgcggtgggt tcgccggggg cgacgtgttc ggctccgcgg agttgtgccg gggcaagctc
     8821 cgcaagttcg tgcggggcca ctacctcgac gcgctcgacg aaggcgtccg ctttcccgcc
     8881 atcacggtcg aggaggtcat cgcgcaggtg cgcgccgccc agcaggagca gttcgccacg
     8941 gtcggcaaag gctccgaggt gcgcttcgag gcccgcgacg tgcagggcac ctggaagctc
     9001 gtggacgacc tcatcccgca cctgctcgta ctgccgaagc tcaactaacg gcaaaaaggg
     9061 taaggggtaa aggggaaggg gtaagggaaa agaagttgtc tttcccttac cccttcccct
     9121 ttac
//
LOCUS       DASVES010000264         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_974544, whole genome shotgun sequence.
ACCESSION   DASVES010000264 DASVES010000000
VERSION     DASVES010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974544"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VF240_10590"
     CDS             complement(<1..457)
                     /locus_tag="VF240_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7175696.1"
                     /translation="MINFETAPERYHHWKLSVDGAVATLTMDVQEDATLAEGYKLKLN
                     SYDLGVDIELADAVQRLRFEHPQVRCVVVTSLKPRIFCAGANIYMLGTSSHAFKVNFC
                     KFTNETRCAIEDASRNSNQRYLAALNGTASGGGYELAIACDEIYLVDDGN"
     gene            complement(682..755)
                     /locus_tag="VF240_10595"
     tRNA            complement(682..755)
                     /locus_tag="VF240_10595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(719..721),aa:Arg,seq:tcg)
     gene            complement(793..1299)
                     /locus_tag="VF240_10600"
     CDS             complement(793..1299)
                     /locus_tag="VF240_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7175697.1"
                     /translation="MTTLKIALCLAACAMLVAACTTAPESNQMAGANNNTARPTNAPA
                     ATPTAARPAPTADELAAIRGVYAKECQRCHKPDGAGGVFEEEGLKPLKVAGLRTGSAV
                     RHTDEQLARKISNGDIDEGMPAFKSKLTPEQINELVRFIRVEFQSGAAGTGNTNAPVN
                     ANSPASAR"
     gene            1581..2363
                     /locus_tag="VF240_10605"
     CDS             1581..2363
                     /locus_tag="VF240_10605"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX7175698.1"
                     /translation="MSTSEKDNQGGGRSSLRLAERPSRHEGLEQPQPSLPRQFVTFTF
                     YHVRPDWRALDDTEKQELKRAFVETAESFRKDLLINSYSITGLRSSVDFMIWRIGHAL
                     DPFQEMSARLNATGLGRHLEVAQSFLSMTKRSMYIDKDNPEHVEDRLHIVPGKSKYLF
                     VYPFVKTRAWYALPVEQRQEMMDEHIRVGSKYPSVKLHTTYSFGLDDQEFVVAFETDE
                     PSDFLDLVQELRETKASSFTLRDTPMYTCRRRTLAECLDALG"
     gene            2685..>2933
                     /locus_tag="VF240_10610"
     CDS             2685..>2933
                     /locus_tag="VF240_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175699.1"
                     /translation="MEAENMRGLATDARNEPRIYASYVLHKRESAPGWGINGPGVSAE
                     WSQGGESEWNSVAASPDEVKATIYRDVEIPRDGRYRVWV"
BASE COUNT          545 a          865 c          975 g          548 t
ORIGIN      
        1 agttgccgtc gtccaccaga tagatttcgt cgcaggcgat ggccagctcg tagccgccgc
       61 cggaggccgt gccgtttaag gcggcgaggt atcgctgatt ggagttgcgc gaggcgtcct
      121 cgatggcgca gcgggtctcg ttggtgaact tgcagaagtt gaccttgaag gcgtgcgacg
      181 aggtgccgag catgtagatg ttggcgcccg cgcagaagat gcggggcttg aggctggtga
      241 cgacgacgca gcgcacctgc gggtgctcga agcgcaggcg ctggacggcg tcggccagct
      301 cgatgtccac gccgaggtcg taggagttga gcttgagctt gtagccttcg gcgagcgtgg
      361 cgtcctcttg aacgtccatc gtgagcgtgg cgactgcccc atcaacgctc agcttccagt
      421 ggtggtaacg ctccggcgca gtctcgaaat taatcatcaa tggcctgacc ccgaactaaa
      481 cacagactat gtagaacgaa aatattcggt gctgaataag cgacaaatta tagcggctgc
      541 gaggcggaaa tgcattccgg cgcgctaccc gcatcgtcaa tatgtgcaag ggtgagttgc
      601 cccattgaag ctggcgcact ataagcgcac caaacaagaa agggcagccg cgcggctgcc
      661 ctttcttgtt gatactgtaa atgcacccgg aaggactcga accttcggcc ttctgattcg
      721 aagtcagacg ctctatccaa ctgagctacg ggtgcaggcg atgcgtcacg cgaagcatat
      781 cacaacgaga agtcagcggg cgctggccgg agagttggcg ttgacgggcg cgttcgtgtt
      841 ccccgtgccg gccgcgccgc tctggaattc gacgcggatg aagcggacga gttcgttgat
      901 ctgctcgggc gtgagcttcg acttgaaggc gggcatgcct tcgtcaatgt cgccgttgct
      961 gatcttgcgc gcgagctgct cgtcggtgtg ccgaaccgcg ctgcccgtgc gcaggcccgc
     1021 caccttgagg ggtttgaggc cctcttcctc gaagacgccg cccgcgccgt cgggcttgtg
     1081 gcaacgctgg cactctttgg cgtagacgcc gcggatggcg gcgagttcgt cggcggtcgg
     1141 cgcggggcgc gcggccgtcg gcgtggccgc gggggcgttg gtaggacgcg cggtgttgtt
     1201 attagcgccg gccatctggt tcgactcggg cgcggtcgtg caggcggcga cgagcatggc
     1261 gcacgcggcg aggcagaggg cgatcttgag cgttgtcatc cggtgtgtgg tctccgaaat
     1321 tgcaatcgac ttggttggcc gtaacggcga gagtataacg cagcggcgtc ggcgtttgta
     1381 aagtgcgggc gctgctgcgt cggggccgtg tcgtgtactt cgttcattgt gacaggacgg
     1441 aggccatgcg tgcggggcgc gtttgctttc tcgcgtcgcg ccccgcaaaa tgattttagt
     1501 ggcccgacgg catttaaacg attctgacac tcaccgcccg gtcaagtagg tggccggcgc
     1561 ttggagttag acagaggttt atgagcactt ccgaaaaaga caatcagggc gggggacgct
     1621 cctcgttgcg gctggcggag cggccgtcgc gccacgaagg tctggagcag ccgcagccgt
     1681 ctctgccgcg gcagttcgtc accttcacct tctaccacgt gcgccccgat tggcgcgcgc
     1741 tcgacgacac ggagaagcag gagctgaaac gcgcgttcgt cgagacggcc gaatccttcc
     1801 gcaaagattt actcatcaac agctattcga tcacgggcct gcgcagcagc gtggacttca
     1861 tgatctggcg catcggccac gcgctcgacc cgtttcagga gatgtcggcg cgtctcaacg
     1921 ccacggggct tggccgccat ctcgaagtcg cgcagagttt cctgtcgatg accaagcggt
     1981 cgatgtacat cgacaaggac aacccggagc acgtcgagga tcgcctgcac atcgttcccg
     2041 gaaagtcgaa atacctgttc gtctacccgt tcgtgaagac gcgcgcatgg tacgcgctgc
     2101 ccgtcgagca gcggcaggag atgatggacg agcacatccg cgtcggctcg aagtacccga
     2161 gcgtgaagct tcacacgacc tactccttcg gcctcgacga tcaggaattc gtcgtcgcct
     2221 tcgagaccga cgagccgtct gatttcctcg acctcgtgca ggaactacgc gagacgaagg
     2281 ccagcagctt cacgctgcgc gacacgccga tgtacacctg ccgtcggcgt acacttgccg
     2341 aatgcctcga cgcgctcggg tgagcggccg cgatacagac acgctcggca gacgcggagg
     2401 gacaacaagc ccctccgcgt ctcttcgttt ccggcaacac cacaccaatg ccctccgcgc
     2461 cttggcgtcc tctgcgttaa attttcgacc ccaaaggaaa tttaacgcag aggacgccaa
     2521 ggcgcggagg acgctgagat gagcgtagtt cgcacaatat gcgtcgcaga acaaatagcg
     2581 ggtcagcact gttagccctg tgtctcgtgt tccagggagc gacggcgcag actgtgacgc
     2641 cgagtcaggc gggcggcgca cgtcagcgtt tcgagtatct atggatggag gccgagaaca
     2701 tgcgcggcct cgcgacggac gcgcgcaacg agccgcgcat ctacgcctcc tacgtcctgc
     2761 acaagcgcga gagcgcgccg ggctggggca tcaacgggcc gggcgtctcc gccgaatggt
     2821 cgcagggcgg cgagagcgag tggaactccg tggcggcgag tcccgacgag gtgaaggcca
     2881 cgatttacag ggacgtggaa atcccgcgcg acgggcgtta ccgcgtctgg gtg
//
LOCUS       DASVES010000265         9377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_397932, whole genome shotgun sequence.
ACCESSION   DASVES010000265 DASVES010000000
VERSION     DASVES010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397932"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1234
                     /locus_tag="VF240_10615"
     CDS             143..1234
                     /locus_tag="VF240_10615"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX7175700.1"
                     /translation="MSTNERMQAVPEAEAAAARARDAQADARDGAAKTEELVRVRGLF
                     KHFPVEGSDDVVRAVDGLTFDIFRGETLGLVGESGCGKSTVGRCLLRLIEPTRGEVSF
                     EGRDVLALGGGELRKLRREMQIIFQDPYASLNPRLKVRDIVSEPLVIHGIGDKTERRE
                     RVADLLRKVGLDPDYMNRYPHEFSGGQRQRIGIARALALNPKLIVADEPVSALDVSVQ
                     AQVVNLLEDLQKEFDLTYFFISHGLAVVEHISDRVAVMYLGRIVEVASAEDLYANPLH
                     PYTRALLSAIPIPDPTRKRERIVLKGDVPTPINPPSGCRFHTRCPEAIPECAAVDPDL
                     REVAPGHTVACIRVPGWADAPTGTVSSEQ"
     gene            1431..2360
                     /locus_tag="VF240_10620"
     CDS             1431..2360
                     /locus_tag="VF240_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX7175701.1"
                     /translation="MIRRTYALVALGVGLALVAAAMLLKVLPGGFVVIGASLAFFGLL
                     ATGLSFVPRPEAAPDAPAPMPAFERIAGLFYRPAEVFRNLRAHPRWLTALLIITLCSF
                     IYTTAFTRRITPERIADHTTQKVIETGWVKEADAAKMKEQQIEAAKDPVRVWGGVVGQ
                     FSFGFVLIAFLAGLFLLGVMALGGRINFWQALAIAAHAYLPVFVIQKLLSLLLLYLKS
                     PDDIHPMLGAQGLVQDNLGVLFSPAEHPVLFAAASAFGVLSFYGLWLLATGLKNGGER
                     VSKTTAWSMAIGIWVIGLLLAVSSAALFGSFMA"
     gene            complement(2448..3707)
                     /locus_tag="VF240_10625"
     CDS             complement(2448..3707)
                     /locus_tag="VF240_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7175702.1"
                     /translation="MNIFRSDIYENLMMALATLRANKLRSALTVVGVIIGVWTVMAIA
                     SIISGIDMAVKKEVEQFGTRSIFLYKFDIGPRTGDLTREERMRPNLTYDDSIAISDLS
                     AVESTFPFLNISNQMFRGQRLMVKKGGKSSGSVQIEGTTPGYDRSGIITMAEGRFFVE
                     IENDTSQEVCVIGASVADNFFPYGSPVGQTIEVGAKEFRVLGVMMKREQMGGGDGGDD
                     LNNIIFVPFKVAQQLKPNADDLFILAVARPGFTDAAKDQIEELLRRRRGVAFDQPNNF
                     GMATAESIVATFRSIMAGVAIAMVVISSVGLMVGGIGVMNIMLVSVTERTREIGTRKA
                     VGARRRDILWQFLIEAMTLTGAGGLIGLLLGWGTTFLISLFIPSYVPLWAPAAGFFAS
                     VGIGLIFGLWPAWKAARLDPIEALRYE"
     gene            complement(3733..4026)
                     /locus_tag="VF240_10630"
     CDS             complement(3733..4026)
                     /locus_tag="VF240_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175703.1"
                     /translation="MTVVIEFLIVALALYLCASIWAPGIRPYWRAGVLDIRSRLGTLS
                     SVGAAVFFGTPAVIILSGPPATGRGLLYVCAMILGLALTVAGYCIDKKVADKA"
     gene            complement(4101..5345)
                     /locus_tag="VF240_10635"
     CDS             complement(4101..5345)
                     /locus_tag="VF240_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7175704.1"
                     /translation="MNFAETLKLALAAIRAHKLRSFLTLLGMIIGVTAVVVVISMIQG
                     FNTYVDEKIANIGANNFTIFRFNFFTDFQNTDTLAAAARRNKELTLDDLEFLRERRVV
                     IDQLGARVNGVSAEIKRGGEELQGVPLSGATPDMAAIENITVEFGRFFSEQENEAAMR
                     VAFIGWDVADKLFTAGAASAVDQEISIRGIPYRVIGVAVAKGTVFGRPQDNFVAVPMK
                     SFIRQFGPPGRNRWVYFAGTPRSPGLYKDAVEEARQLMRLRRQLRGDETDNFAVITPD
                     AITGARDRIFGPIFIVAIVVPGIALLVGAIVIMNIMLVSVTERTKEIGIRKALGARRS
                     DVLKQFLVEAVTLSAIGGGIGVLLAWLIGLGITAYVFQTRMSAVSILLAVGVSGGVGV
                     LAGIFPAWKAARLDPIEALRAD"
     gene            complement(5478..6254)
                     /locus_tag="VF240_10640"
     CDS             complement(5478..6254)
                     /locus_tag="VF240_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7175705.1"
                     /translation="MEPPTTDTTTTITTDPAALERAELLAHDAVPAALISMRDIWKTY
                     QMGEEPLHALRGVTFDVQRGEYLAIIGPSGSGKSTLMNLIGCLDTPSQGRYWINSKLV
                     SDMDDDELARIRNKEIGFVFQTFNLLARATALHNVELPLIYAGISSGERHEKAKRALS
                     SVELSDRMMHRPNELSGGQRQRVAIARALVNHPSLLLADEPTGALDTKTSHEIMSLFE
                     RLHAEGNTIIVVTHEHDVAERAHRVVHIRDGLIEKDERIK"
     gene            complement(6269..7831)
                     /locus_tag="VF240_10645"
     CDS             complement(6269..7831)
                     /locus_tag="VF240_10645"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7175706.1"
                     /translation="MALSRKKKIIIAVSAVAVLGLIVIISVFARGTDQPEVTVVKVET
                     RAELKSTVTASGEVRPIQFINLTSEVGGRIEEIYVNPGDQVQKGQPLVRLDPTQLQSQ
                     QEAQAAGVQMAVSDMASARTAVQSAETNVQQAQQGLAAAEVGLSNARQGIVTAQTNVD
                     REQVNLTTAQRELKRTTELVESGVSSRFEFDAARDRAEQAQVALRTAQAQLDSAKISV
                     EEAKARVNQERVRVKSAQEGVVSARSGVRTSESRVSMEQARLRGETSQRGKSMQISPL
                     TGVVADIPARVGQFALANFSTTPLMTIADMSAINVEVNVDETEIDEVVENQPVKIKVD
                     ALGERELEGVVKQKTPLAISKSDTAGAGLGGRVNVQEAKEFKVVILLDRAKTPDEFWN
                     ALKPGMTATAVITTKVKQNVVAVPLQAIVEKLPTPTPTPPAGSAPAPPSERPKPIKGV
                     YVLDGNKAKFVAVETGITGESDIEITSGLEANVEVITGPSRVLRTLKEATVVKKQERK
                     PGQPEGPEDAKS"
     gene            8266..9027
                     /locus_tag="VF240_10650"
     CDS             8266..9027
                     /locus_tag="VF240_10650"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7175707.1"
                     /translation="MICQRCNTENLDGAQYCDECGGLLASASRSRQKPDTEPQDNGAA
                     GERRAGAAAETRPAETGARNGDARSGFLSEDISPRGNSGRTNTYGTRPGAAEQGEAVA
                     SPPRAASLGAFGSSSGSHESRAGAVASAGNVSAQQTGAGGGPHAKLVIHRGRSVGKEF
                     PMSEDETQIGRWDADGGIFPDVDLDADDPEAKVSRRHARITRRGEQYFVEDLGSTNGT
                     FINRGRRLLPGDRHPLRDGDEVIVGKTFLRFQVLK"
     gene            complement(9125..>9377)
                     /locus_tag="VF240_10655"
     CDS             complement(9125..>9377)
                     /locus_tag="VF240_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175708.1"
                     /translation="TSSVFGAGVTAGAPPTLASGSETGDDSASAPDEDDDAAEALAPS
                     GSRLEGAPVPGCESVTPQLPQKRSSAVTAVPHWKQNAIP"
BASE COUNT         1550 a         3043 c         3104 g         1680 t
ORIGIN      
        1 gctctacgac ctcgcggcgg ccgtcgccgc agaccaccag atacaggtga taagtgatga
       61 gtgataagtg ataagaaaga agaggacagc acgctttctc tttcttatca cttatcactc
      121 atcacttatc actacctaag acatgagcac taacgaaaga atgcaagccg tccccgaagc
      181 cgaagcggcg gcggcccgcg cgcgcgacgc gcaggcggac gcgcgtgacg gcgcggcgaa
      241 gacggaagaa ctcgtgcgcg tgcgtggatt gttcaagcac ttccccgtcg agggcagcga
      301 cgacgtggtg cgcgccgtgg atggtctgac gttcgacatc ttccgcgggg agacgttggg
      361 gctggtgggc gagtcgggct gtggcaaatc gaccgtcggg cgctgcctgc tgcgactcat
      421 cgagccgacg cgcggggaag tctccttcga gggccgcgac gtgctcgcgc tcggcggcgg
      481 cgagttgcgt aagctccgcc gcgagatgca gatcatcttt caagaccctt acgcctcgct
      541 caatccgcgt ctcaaggtgc gcgacatcgt ctcggagccg ctcgtcattc acggcatcgg
      601 cgacaagact gagcggcgcg agcgcgtcgc cgaccttttg cggaaggtcg gactcgaccc
      661 cgactacatg aaccgctacc cgcacgagtt ctccggcggc cagcgccagc gcatcggcat
      721 cgcccgcgcg ctcgctctca acccgaagct tatcgtggcc gacgagccgg tgtcggcgct
      781 cgacgtgtcg gtgcaggcgc aggtcgtcaa cctgctcgaa gacttgcaga aggagttcga
      841 cctcacctac ttcttcatct cgcacggcct cgccgtcgtc gagcacatct ccgaccgcgt
      901 cgccgtgatg tatctgggcc gcatcgtcga ggtggcctcc gccgaagatt tgtacgcgaa
      961 cccactgcac ccttacacgc gcgcactgct ctcggccatc cccatccccg accccacgcg
     1021 caagcgcgag cgcatcgtgc tcaagggcga cgtgcccacg ccgattaacc cgccctcggg
     1081 ctgtcgcttc cacacacgct gccccgaagc catcccggag tgcgcggcag tagaccccga
     1141 cctgcgcgaa gtcgcgcccg gccacaccgt cgcctgcatc cgcgtccccg gctgggccga
     1201 cgcgccgacg gggacagtga gcagtgagca gtaagcagtg agcagtaagc agtcaaggct
     1261 ggttatacgt cctgactgct tactgctcac tgcttactgc ctactgtctt attgaaacct
     1321 cgccttaagg cgctgcgtat caaatgggtc tgctggtcgg tgggatggaa ggtgaggacg
     1381 cgcgcccgcg tccacttcac cgccgcttta acgcgaggag gattccccca atgattcgtc
     1441 gcacgtatgc actggtggcg ctgggcgtcg gccttgcgct ggtggccgcc gctatgctgt
     1501 tgaaagtgtt gccgggtggc ttcgtcgtca tcggcgcgtc actggccttc ttcgggttgc
     1561 tggcgacggg cctgagcttc gtgccccggc cggaagccgc gcctgacgcg cccgcgccga
     1621 tgcccgcctt cgagcgtatc gccgggttgt tctatcggcc ggccgaggtc ttccgaaacc
     1681 tgcgcgcgca cccgcgctgg ctcacggccc tgctcatcat caccctctgt agcttcatct
     1741 acacgaccgc cttcacgcgc cgcatcacgc ccgagcgcat cgccgaccac acgacgcaga
     1801 aggtgatcga gaccggctgg gtgaaggagg cggacgccgc gaagatgaaa gagcaacaga
     1861 tcgaggccgc caaagacccg gtgcgcgtgt ggggcggcgt cgtcggccag ttcagtttcg
     1921 gcttcgtgct catcgcgttc ctcgccggac tcttcctgct cggcgtgatg gcgctggggg
     1981 gccgcatcaa cttctggcag gcgctggcca tcgcggccca cgcctatctc cccgtcttcg
     2041 tcatccagaa actcctcagc ctcctgctgc tctacctcaa atcccccgac gacatccacc
     2101 cgatgctcgg cgcgcagggt ttggtgcagg acaacctcgg cgtgctcttc agcccggccg
     2161 agcaccccgt gctgttcgcc gccgcgagcg ccttcggcgt cctctccttc tacggcctgt
     2221 ggttgctggc gacgggcctc aaaaacggcg gcgagcgcgt gagcaagacg accgcgtggt
     2281 caatggccat cggcatctgg gtcatcggac tcctcctcgc cgtcagctcg gccgccctct
     2341 tcggcagctt catggcgtaa cacgaagggt aaggggtaaa ggggaaaggg taagggggaa
     2401 aggcacgtca acctttcccc cttacccttt cccctttacc ccttttctta ttcgtatctg
     2461 agcgcttcga tagggtcgag gcgggcggcc ttccacgccg gccagaggcc gaagatgagg
     2521 ccgatgccga ccgaggcgaa gaagccggcg gcgggcgccc acagaggcac gtaggagggg
     2581 atgaagaggc tgatcaggaa ggtcgtgccc cagccgagga ggaggccgat gagaccgccc
     2641 gcgccggtga gcgtcatggc ctcgataaga aactgccaga ggatgtcgcg gcggcgcgcg
     2701 cccacggcct tgcgcgtccc gatctcgcgg gtgcgctccg tcaccgacac cagcatgatg
     2761 ttcatcacac cgatgccgcc gaccatcaac ccgaccgagg agatgacgac catcgcaatc
     2821 gccacgcccg ccatgatcga gcggaaggtg gcgacgatgg attcggccgt cgccatgccg
     2881 aagttgttcg gctggtcgaa ggccaccccg cgacgacggc gcaggagttc ttcgatctga
     2941 tctttcgcgg cgtcggtgaa gcccggtcgg gcgacggcga ggatgaagag gtcgtcggcg
     3001 ttgggcttga gctgctgcgc caccttgaag gggacgaaga tgatgttgtt gaggtcgtcg
     3061 ccgccgtcgc cgccgcccat ctgctcgcgc ttcatcatca cgccgaggac gcggaactct
     3121 ttggccccga cctcgatggt ctgcccgacg ggcgacccgt aggggaagaa gttgtcggcc
     3181 acgctcgccc cgatgacgca gacctcctgg ctcgtgtcgt tctcgatctc gacgaagaag
     3241 cggccctcgg ccatcgtgat gatgccggag cggtcgtagc ccggcgtggt gccctcgatc
     3301 tgcaccgagc ctgaagactt gccgcccttc ttcaccatca ggcgctgccc ccggaacatc
     3361 tggttggaga tgttcaggaa cgggaaggtc gattcgactg ccgagaggtc gctgatggcg
     3421 attgagtcgt cgtaggtcag attggggcgc atccgctcct cgcgcgtgag gtcgccggtg
     3481 cgcgggccga tgtcaaactt gtagaggaag atcgagcgcg tgccgaactg ctcgacctct
     3541 ttcttgacgg ccatgtcgat gccgctgatg atcgaggcga tggccatcac cgtccacacg
     3601 ccgatgatta cgccgaccac cgtcagggcc gagcgcagct tgttcgcccg caaggtggcg
     3661 agcgccatca tcaaattttc gtagatgtcg cttctgaaga tattcataaa gccttcaccc
     3721 gccgctgacc gctcaagcct tgtccgccac cttcttgtcg atacagtaac cggccaccgt
     3781 cagggccagt cctaagatca tggcgcagac gtagagaagg ccgcgccccg tcgccggcgg
     3841 gccgctgagg atgatcaccg ccggggtccc aaagaacact gcggccccga cgctgctgag
     3901 cgtgcccaaa cggctacgga tgtcgagcac gcccgcgcgc cagtaggggc ggatgcccgg
     3961 cgcccagatg ctcgcgcaca ggtaaagcgc cagcgcgacg atgagaaatt ctatgacgac
     4021 tgtcatcgtt ccgaaagcgt cgtcgccaca aaagctcggg cgcgggcggc ggcgaaggct
     4081 tggtcgcgcg cgcatgcgcg ttagtcggcc cgcagggcct ctatcgggtc gaggcgcgcc
     4141 gccttccacg ccgggaagat gccggccaaa acgccgacgc cgcccgagac gccgaccgcc
     4201 agcaggatcg agacggcgga catccgcgtc tggaagacgt aggcggtgat ccctagtccg
     4261 atgagccacg ccagcaacac gccgatgccg ccgccgatgg ccgacagcgt gacggcctcg
     4321 acgagaaact gcttgagcac gtccgagcgc ctcgcgccga gggccttgcg gatgccgatc
     4381 tctttggtgc gctccgtcac cgacaccagc atgatgttca taatcacgat ggcgccgact
     4441 aagagcgcga tgccggggac gacgatggcc acgatgaaga tcgggccgaa gatgcggtcg
     4501 cgcgcgcccg tgatggcgtc gggagtgatg acggcgaagt tgtcggtttc gtcgccgcgt
     4561 aactgccggc gcagacgcat caactgccgc gcttcttcga ccgcgtcttt gtagagaccg
     4621 ggggagcggg gcgtgccggc gaagtagacc cagcggttgc ggcccggcgg gccgaactgt
     4681 cggatgaagg acttcatcgg caccgcgacg aagttgtcct gcgggcggcc gaagacggtg
     4741 cccttggcga ccgccacccc gatgacgcgg taggggatgc cccggatgct gatctcctga
     4801 tcgacggccg aggccgcccc cgccgtaaac agcttgtcgg ccacgtccca gccgatgaag
     4861 gccacacgca tggccgcctc gttctcctgc tcggagaaga agcgaccgaa ttccacggtg
     4921 atgttctcaa tggcggccat gtcgggcgtc gcccccgaga gggggacgcc ctgaagttcc
     4981 tcgccgcccc gcttgatctc ggcggagacg ccgttgacgc gcgcgccgag ctggtcgatg
     5041 acgacgcgcc gctcgcgcag gaattcgagg tcgtcgagcg tcagctcctt gttgcggcgc
     5101 gcggcggcgg cgagggtgtc ggtgttctgg aagtcggtga agaagttgaa gcgaaagatg
     5161 gtgaagttgt tggcgccgat gttggcgatc ttctcatcga cgtaggtgtt gaagccctga
     5221 atcatcgaga tgacgacgac gacggccgtc acgccgatga tcatgccgag cagtgtcaaa
     5281 aacgagcgca gcttgtgcgc ccggatggcg gccagcgcaa gttttaatgt ctcggcgaaa
     5341 ttcacggctg atattctacg ctcaggatga cgaataggtt atcaaactaa agccgtaaat
     5401 cgaaaatcgt gaatcgtaaa tcgtggacgc ttcccacgat ttacgattca cgattttcga
     5461 tttacggctt tgttgcctta cttgatgcgc tcgtccttct cgatgaggcc gtcgcggatg
     5521 tggacgacgc ggtgggcgcg ctcggccacg tcgtgctcgt gggtgacgac gatgatcgtg
     5581 ttgccctcgg cgtggagacg ctcgaagagg ctcatgatct cgtggctggt cttggtgtcg
     5641 agcgcgcccg tcggctcgtc ggcgaggagg agcgacgggt ggttgaccag cgcccgggcg
     5701 atggccactc tctggcgctg cccgccggaa agctcgttgg gccggtgcat catgcggtcg
     5761 gagagttcga ccgacgagag agcgcgcttg gccttctcgt ggcgctcgcc cgaagagatg
     5821 ccggcgtaga tgagcggcag ctcgacgttg tggagggccg tggcgcgcgc gagcaggttg
     5881 aaggtctgga agacgaagcc gatttccttg ttgcggatgc gggcgagttc gtcgtcgtcc
     5941 atgtcggaga cgagcttcga gttgatccag tagcggccct gactcggcgt gtcgaggcag
     6001 ccgatgaggt tcatcagcgt ggacttgccc gaccctgacg ggccgatgat ggcgaggtat
     6061 tcgccgcgct gcacgtcgaa ggtgacgccg cgcagggcgt gcaaaggctc ctcgcccatc
     6121 tggtaggtct tccagatgtc gcgcatcgag atgagcgcgg ccgggacggc gtcgtgcgct
     6181 aatagctccg cgcgctcgag ggcggcgggg tcggtggtga tcgtcgtagt ggtgtcggtg
     6241 gtgggtggtt ccatcagcgt tgcttgcgtc acgacttggc gtcctcgggg ccttcgggct
     6301 ggcccggctt gcgctcctgt ttcttgacga ccgtggcctc cttgagcgtg cgcagcaccc
     6361 ggctcggccc cgtgatgacc tccacgttcg cctccagtcc gctcgtgatc tcgatgtcag
     6421 actcgcccgt gatgccggtc tcgacggcga cgaacttcgc cttgttgccg tcgaggacgt
     6481 agacgccctt gatcggcttg ggccgctcgg acggcggcgc gggggcggag ccggcgggag
     6541 gcgtcggcgt cggcgttggc agcttctcga cgatggcctg aaggggcacg gcgacgacgt
     6601 tctgcttgac cttcgtggtg atgacggccg tcgccgtcat gcccggcttg agcgcgttcc
     6661 agaactcgtc gggcgtcttc gccctgtcga gcaggatgac caccttgaac tctttggcct
     6721 cctgcacgtt cacgcgtcca cccagcccag cgcccgccgt gtcgctcttg ctgatggcga
     6781 gcggcgtctt ctgcttgacg acgccttcga gttcgcgctc gccgagggcg tcaaccttga
     6841 tcttgacggg ctgattctcg acgacctcgt cgatttcggt ttcgtccacg ttgacctcga
     6901 cgttgatggc cgacatgtcg gcgatggtca tcagcggcgt ggtcgagaag ttggcgaggg
     6961 cgaactgccc gacgcgcgcg gggatgtcgg cgacgactcc cgtcagcggc gaaatctgca
     7021 tcgacttgcc gcgctggctc gtctcgccgc gcagacgcgc ctgctccatc gagacgcgcg
     7081 actcgctcgt ccgcacgccc gagcgcgcgc tcaccacacc ctcctgcgcg cttttgacgc
     7141 ggacgcgctc ctgattgacg cgcgccttcg cctcctcgac cgaaatcttg gccgagtcga
     7201 gctgcgcctg cgcggtgcgg agcgccacct gcgcctgctc ggcgcggtcg cgcgcggcgt
     7261 cgaactcgaa gcgcgacgac actcccgact cgaccagctc ggtcgtgcgc ttcagctcgc
     7321 gctgcgccgt ggtgagattg acctgttcgc ggtcaacgtt ggtctgcgcc gtgacgatgc
     7381 cctgacgcgc gttgctcaag cccacctcgg cggcggccag tccctgctgc gcctgctgga
     7441 cgttcgtctc ggccgactgc acggccgtgc gcgcgctcgc catgtcggag acggccatct
     7501 gcacgccggc ggcctgcgcc tcctgctgcg actgaagctg cgtggggtca agccgcacga
     7561 gcggctgacc tttctgcacc tgatcgccgg ggttgacgta aatctcttcg atgcgcccgc
     7621 ccacttcgct cgtcagattg atgaactgga tggggcgcac ctcgccggag gccgtcacgg
     7681 tggacttgag ttcggcgcgg gtctcgacct tgacgacagt gacttcgggc tgatcggtcc
     7741 cacgcgcaaa cacgctgatg atgacgatga ggccgaggac ggcgacggcc gagacggcga
     7801 tgatgatctt cttcttgcgg ctgagggcca tgacttcttc gattccttcg ttaggctaaa
     7861 tcgggttcgc gcgcggggcc ggattagtct tcttgatctc gcacgacggg acgcgaaatc
     7921 aaagctcctg aacgacttca cgtctcgcgc cgcactcttc tacgttcaaa gcgggcggac
     7981 agtttcaaaa accgtgacgc ctccatcggt gacagttgac tgagggttga ggggtcgcgc
     8041 tcgggcagtt ttgatgagcg ggcggcccct tcggttggca cgcgccccat cgcccgccac
     8101 ccgtcatctg tcacggcttt ataatgcctt tggtttcgcg ctacaatgac ctccccggcg
     8161 caagccgcga agtgattcaa gggcgtgttc agtccgacgc cgcgccggga gaaggtggcc
     8221 ccgaggcttt tcctcccgcg cactcaagaa attcaagggc cttcaatgat ctgccaaagg
     8281 tgcaacacgg agaacctcga cggcgcgcag tactgcgacg agtgcggcgg gcttttggcc
     8341 tccgcgtcgc gctcccggca gaagcccgac accgagccgc aggacaacgg cgcggcgggc
     8401 gagcgccggg cgggcgccgc cgccgaaacg cgccccgccg agacgggcgc gaggaacggg
     8461 gacgcgcgct ccggctttct ctccgaagac atttcgccca ggggcaactc gggccgcacg
     8521 aacacctatg gcacgcgccc cggcgcagcc gaacagggcg aggcagtggc gtcgccgccg
     8581 cgcgccgcaa gcctcggcgc gttcggctcc tcctcgggga gtcacgaatc gcgcgccggt
     8641 gcagtggcgt cggccggcaa cgtctcggcg cagcagacgg gcgccggagg agggccgcac
     8701 gccaagctcg tcatccaccg cgggcgctcg gtgggcaaag agtttccgat gagcgaggac
     8761 gagacgcaga tcgggcgttg ggacgccgac ggcggtatct tccccgacgt tgacctcgac
     8821 gccgacgacc ccgaggccaa agtctcgcgc cgccacgcac gcatcacgcg gcgcggcgag
     8881 cagtacttcg tcgaagacct cggctcgacg aacggcacct tcatcaaccg cgggcggagg
     8941 ctccttccgg gcgaccgcca ccccttgcgc gacggcgacg aagtcatcgt cggcaaaacg
     9001 tttttgcgat tccaggttct caagtgagtg tcagcgcgcg catgatggcc catgcgggcc
     9061 ggggcgacag gcgcgcgcgc ctgtaggtta aagtttcggg cgggtaaacc gtagcaggta
     9121 aaacttaagg gatggcgttc tgtttccagt gcggaacggc ggtcacggcc gaagaccgct
     9181 tctgcggcag ttgcggggtc acgctctcgc agccgggcac gggtgcgcct tcgagccgcg
     9241 agccggaggg cgcgagcgcc tcggccgcgt cgtcgtcttc gtcaggcgca gacgctgagt
     9301 cgtcgcctgt ctccgagccg gacgcgagcg tcggcggcgc tcccgccgtg acgcccgcgc
     9361 cgaagaccga tgaagtc
//
LOCUS       DASVES010000266        10157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1557789, whole genome shotgun sequence.
ACCESSION   DASVES010000266 DASVES010000000
VERSION     DASVES010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557789"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..686
                     /locus_tag="VF240_10660"
     CDS             99..686
                     /locus_tag="VF240_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175709.1"
                     /translation="MYPHERALPQHYIVGATWLLLGLVVGLHFPDVDLRLQGLIPSRV
                     LLHRSILTHGLIASVMLFWLIRSRRDAVPAHRLFVVGVSLAVAVHLCFDFFPRGWSGF
                     ALIHIPIYGRTTALFSQSWLILSIILCLYVAFLLVRNIIELALSIGHLLISLTVSAVK
                     NKHPTTHLAVMLLALAVIVTVLMAHRVRKVAARRA"
     gene            866..2188
                     /locus_tag="VF240_10665"
     CDS             866..2188
                     /locus_tag="VF240_10665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7175710.1"
                     /translation="MDNGELRLRHKILVTMSSILLWALAVVAVVFAEAKWFAHPSVVR
                     GELASIENHLVKKLGDAAENKKLGSAALILVQGSKIAAEHSFGVANAETQAPVKSDQT
                     LFLVASVSKAVTAWGVMKLVEEGKLGLDEPVMPRLTRWRFPGSGAHRDKVTVRHLLSH
                     TAGLDDGLGYGGFLPGEAIQTLEESLTLTKDSTVGEPRGVTVEREPGEGMAYSGGGYT
                     VLQLLIEEVTLRPFADYMKESVLQPLGMTKSSFDLDAIASEGRAQDLATSFDRELQAH
                     PHRRYTAKAAVSLYATPRDLAQFARAFAGENPVLRQETLKQMMLPQPGTSGTWGLGQT
                     LYAENETGGHVVGHSGGTNPAWGAMLRVNPATGNGMVLTISGGQGTLNQLVHDWVYWE
                     TGKVSFEAQRQVVYDRLAPASVAIILGALVLVWWKLLRKIQGREPVGV"
     gene            2255..3148
                     /locus_tag="VF240_10670"
     CDS             2255..3148
                     /locus_tag="VF240_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7175711.1"
                     /translation="MKHFRLVLGALMSVTAISIACSTITNSPASTPNPGQIVAPGDAA
                     VSRQQQTSLEITYVANEGVLISSGGKRVLIDGLHREYETYASLPAAQRELIETAKAPF
                     DNIDLLLVSHMHLDHFHPESVGLHLRHNPKTMLVSSQQVIGEVEKHFKDYEAIKARVT
                     GATPPLKEKVFMKVAGVDFEILGLGHGSERHGSIQNLGHLIKLGGRKLLHVGDADTSA
                     EIFEKFNLDEEGIDIAFLPYWFLLGDAGQTVVREHIKPKHIVAVHLPTDAEKVAGQIR
                     KIFPDAAVFTTMLEKRHYYTK"
     gene            3210..3812
                     /locus_tag="VF240_10675"
     CDS             3210..3812
                     /locus_tag="VF240_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175712.1"
                     /translation="MKLPLLLSVMLSFSLGHAGFGSVKFDYLKVRGNIAESTHKAKYK
                     LEVDRSYKPLGEFHHQPVYEGVQFNVSLAAFSRGESIIMMHAEMHTDGSGGLDYSKLK
                     PETLDGIKFTSREQCATPAEVPDPYSNPEFRFLRDKGFKAVLPIYLRQYFATSPDGVA
                     EVVLTYGKRVSSCGDDTITPEFKAEVDREVRSSMKVSMKR"
     gene            4164..4676
                     /locus_tag="VF240_10680"
     CDS             4164..4676
                     /locus_tag="VF240_10680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7175713.1"
                     /translation="METLCADEPPGAEFRSTHRHVEAYVRLLETHAPVHRQWAGPAYR
                     FTEYLEPSRPLLAITETNAEMLRGGFEELIAELPECQPFVAVVEDGMAVSVCRSVRIT
                     SAAHEAGVETLPDFRGKGYAKDAVAGWARLVRSVGATPLYSTSWENTASQALAKKSRL
                     VLYGSDFHVT"
     gene            complement(4695..5273)
                     /locus_tag="VF240_10685"
     CDS             complement(4695..5273)
                     /locus_tag="VF240_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175714.1"
                     /translation="MKKTEWKSRTKLDLMIEVWEALDCESVGAQELEAVSEAVGARFG
                     EGAVESPARVARVLADEGAELRHAEVLELDARWRTADPYDAMFRNVLKFSTFDEAVAS
                     LKRLDNLRREFKRRSDREGQRRVHETVLRGKQRAQMIARNQSVNERKRAEKAEIAEWF
                     TVWLRQPEIFDDWVVLRRRSPEFREQFAGDEV"
     gene            5436..5600
                     /locus_tag="VF240_10690"
     CDS             5436..5600
                     /locus_tag="VF240_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175715.1"
                     /translation="MLNPILALTLGRVHEGVGRAPSSALRAPCLQMISGKQYAAPYYW
                     APLVLVGDWR"
     gene            5759..6142
                     /gene="ytxJ"
                     /locus_tag="VF240_10695"
     CDS             5759..6142
                     /gene="ytxJ"
                     /locus_tag="VF240_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HEX7175716.1"
                     /translation="MGEQFVTVSNAGELEQLFARSHREPVLLFKHSTTCPISAAAYQQ
                     MLGVEREASLVVVQKARDLSREIETRTGVRHESPQALILRDGAVVWSASHWNITADAV
                     TRALGEHAESGAKSQEPKVRSQESE"
     gene            6234..6578
                     /locus_tag="VF240_10700"
     CDS             6234..6578
                     /locus_tag="VF240_10700"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX7175717.1"
                     /translation="MSDTAPRRGPEVEPQEIMQDGDRQLRVTWADGRACLYGAAALRR
                     ACPCAQCVNEWTGERVLAPEAVSDDLEISDLQLVGRYALNFRWSDGHETGIYSFRYLR
                     ELCEQSKEISDE"
     gene            6658..7020
                     /locus_tag="VF240_10705"
     CDS             6658..7020
                     /locus_tag="VF240_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7175718.1"
                     /translation="MPKKILIVEDDDVERELVRMTLERQGYAVETAEDGISGYELAVS
                     YRPDLIVADLQMPAADGVHLVRRVRDTAEIAATPVVIVSGMGFGEAAYTLTLGADAYV
                     PKPLDPESLIETVRRLLS"
     gene            7086..7346
                     /locus_tag="VF240_10710"
     CDS             7086..7346
                     /locus_tag="VF240_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX7175719.1"
                     /translation="MTEAVGWASSCILVLTIAKQVYKQWKEGSSEGVSRWLFIGQMAA
                     SLGFTIYSWLVGNWVFVVTNAVMLLSGIAGLLIVLHHRRREA"
     gene            7495..7761
                     /locus_tag="VF240_10715"
     CDS             7495..7761
                     /locus_tag="VF240_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175720.1"
                     /translation="MNERDFFDERQETKPANYSCPHCRERDDYEVRWLRRTRKHQPPR
                     GANEMDRARFQKSRDYMVRVDDQLVCKRCRRRFDIPNMQTVVFI"
     gene            7826..8056
                     /locus_tag="VF240_10720"
     CDS             7826..8056
                     /locus_tag="VF240_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175721.1"
                     /translation="MDELIKQVAERTGIGEDKARTAVDTVVGFLKERLPAPIAGQLDN
                     VIGGGGAASGGGIADQAGDVLGSLGGMFGKKD"
     gene            8265..9788
                     /locus_tag="VF240_10725"
     CDS             8265..9788
                     /locus_tag="VF240_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175722.1"
                     /translation="MLWTMRLVIFVFALVFGAGCWYVFAQTPSAQTTNTNVNGNMNAN
                     NASAEDAAADWTPVPTNTNNLPDDQSAEAHEEEAAPALEWAEPNPAYGHVVAPRVTLR
                     SESNASSNIIATLDVVENESVEIMDSTRDFLRVKFPANTEMEGGTRQQDYDGWVEWGA
                     VVPYTNALVVETESGEVIGRVALDYGISTAAFSPDGSRAVFYGASGGDGHGESQSLAY
                     EFDTESYKILRTLKTSTSGGFASILYDDGGDLRPLLQTHLTSGDSMRKMLQPFRVKGD
                     LFERSGDTIRPGADGPLLFSRDGATGFDIHSADEAPGAISVDVIDVKSFTVRNSFPVE
                     GTASEPWSYEYAVSPDGAEFFYRESEGSIRVVDTRTGLRVRETRPRVAEDFRGYLAND
                     SVVGDSLLIRYWQESESEEGGELRSFWLKADGETTPAERGINNVVEAGGALYAVNDAG
                     TRLFKLDADGRITERLKIARPELKHRPKGSEEHTIMGLYASPDGKHIVIFFGIPDGC"
     gene            complement(9840..>10157)
                     /locus_tag="VF240_10730"
     CDS             complement(9840..>10157)
                     /locus_tag="VF240_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7175723.1"
                     /translation="SAGAFSRDFGLRDQIRRAAVSIPTNIAEGFERSSRKEYLLFLNI
                     AKGSAGEVRSLLRVALEVGYLGQSKHDELREFVSSLSRQLSNQIKAIKEKEEREKKPD
                     KPR"
BASE COUNT         2041 a         2941 c         3146 g         2029 t
ORIGIN      
        1 tcggctcggc cgcccataac atcgacccac tcgtcataat aggagttggg tcgccgaagt
       61 cggctcaatt gttttgcctc ttagcattgt agggcgctat gtatcctcac gaacgagctt
      121 tacctcagca ctatatcgtc ggggcgacat ggttactgtt aggtctcgtc gtcgggctac
      181 atttccccga tgttgatctc aggcttcagg ggctgattcc ttcacgggta ttgcttcatc
      241 gctcgattct gacccacggg ctgatagctt ccgtgatgct cttctggctg attagaagtc
      301 gcagggacgc agtgccggcg caccgattgt tcgttgtcgg ggtgagtctg gcggttgcgg
      361 ttcacctatg ctttgatttt ttcccgcgcg gctggagcgg cttcgcgcta attcacattc
      421 ccatttatgg ccggacgacc gccttgttct cgcaatcatg gctgatactt agcatcattc
      481 tatgcctgta tgtcgccttc ctgctggtac ggaatatcat cgaattagct ctcagtatcg
      541 ggcacctgct gatctccctc accgtttctg ccgttaagaa caagcatcca acaactcacc
      601 tagccgttat gctgttggcg ctcgccgtca tcgttacagt cctcatggcg catcgggttc
      661 ggaaagtggc ggcgcgacga gcctgacaac tccgtacacc cgaccgcgaa tctgcgtgcc
      721 tctcatcgct agacttggct gtatcagagt tgtgcgcgcg gcggttgatg ccgggcgtta
      781 ggtgtctgcc cccggcgaag tgagactgaa agatagttct ggaagaaaaa gccgactacc
      841 cacgtctaac cattaaatcc ctggtatgga taatggagag ctgcgcttgc gtcacaaaat
      901 tttggtcacg atgagttcga tcctgctttg ggctttggct gtagttgccg tggtgttcgc
      961 cgaggcgaaa tggttcgcgc acccgtcggt tgtccgaggg gagttggcgt cgattgagaa
     1021 tcatctggtg aaaaagctgg gcgacgccgc ggaaaataag aagctgggca gtgcggctct
     1081 gatattagta caaggcagta agatcgccgc ggaacatagt tttggtgtcg cgaatgctga
     1141 aacacaagcc cctgtgaagt ccgaccaaac gctttttctg gttgcctcgg tgagtaaagc
     1201 tgtgaccgcc tggggcgtca tgaagctggt ggaggagggc aagcttggtt tggatgagcc
     1261 tgtcatgccc cgcctcacgc ggtggcggtt tccggggagt ggcgcgcatc gcgacaaagt
     1321 gaccgtcagg catctgctga gtcatacggc cgggttagac gacggccttg gctatggcgg
     1381 ttttttaccc ggtgaggcga tacagacgct ggaggaatca ctcaccctga ccaaagattc
     1441 gacggtgggt gagcctcgcg gtgtcacagt ggaaagagag ccgggagagg gcatggctta
     1501 ttcggggggc ggctacactg tcttgcagct cctgattgaa gaggttacgc ttcggccatt
     1561 cgcggactac atgaaagaat ctgttttgca gccgttgggg atgacgaagt cgagttttga
     1621 tttggacgcc atcgcatccg aaggccgggc gcaggacttg gccaccagtt ttgacagaga
     1681 gctgcaagcc catcctcatc gccgctatac ggctaaggcc gccgtttcgt tgtacgcgac
     1741 gcctcgggac ctggctcagt ttgcccgcgc ctttgccgga gagaatccgg tgctcaggca
     1801 ggagacgctg aagcagatga tgctgcccca gccggggaca tccggaacgt ggggtctggg
     1861 ccagactctt tatgctgaga atgagacggg cggccacgtg gtcggacata gtggtgggac
     1921 gaatcccgcg tggggcgcga tgttgcgggt gaacccggcg accggcaatg gcatggtcct
     1981 gacgatttca ggaggacaag ggacgctcaa tcaactcgtg catgactggg tttactggga
     2041 gacgggaaag gtatcattcg aggcgcagcg acaggtcgtc tacgaccggc tggcgccggc
     2101 gtcagtggcg attattttgg gagcactcgt gctcgtgtgg tggaagctgt tgaggaagat
     2161 tcaaggtcgc gagccggtag gggtttaaag ttatgtgccg cgggggagcg tgcagtgaca
     2221 gggcggggac ggacgtagaa agggttgatt gacagtgaaa catttccggt tggttctcgg
     2281 cgccctgatg tccgtgacgg ccatctcgat tgcctgctcg acaattacga acagtccggc
     2341 gagcactccg aatccgggcc agatcgttgc gccgggggat gctgccgtct cccggcaaca
     2401 acagacgagt ttggagatca cttacgtcgc caacgaaggt gtgctcattt catcgggcgg
     2461 taagcgagtg ttgattgatg ggttgcatcg cgaatatgag acgtatgcgt ctttaccggc
     2521 ggcgcagcgc gaattgatcg agaccgcgaa agcgcccttc gacaacattg atctgctcct
     2581 cgtgtcgcac atgcatctgg atcacttcca ccccgaatcg gtcgggcttc atttgcggca
     2641 caacccgaaa acgatgctgg tctcttctca gcaagttata ggtgaggtcg agaagcactt
     2701 caaagactat gaggccatta aagcgcgggt gacgggcgcg actccgcctt tgaaagagaa
     2761 ggtcttcatg aaggtggccg gagttgattt tgaaatcctg ggactggggc acggcagcga
     2821 acggcatggc tcgattcaaa acctcgggca cctcatcaaa ctcggcggca ggaaactact
     2881 ccacgtcggc gacgcggata cgtcggcgga gattttcgag aagttcaatt tggatgagga
     2941 aggaattgac attgctttcc tgccttactg gttcctactt ggtgatgccg gacagacagt
     3001 cgtcagggaa cacatcaaac caaagcacat cgttgccgtc cacctcccca ccgacgccga
     3061 gaaagtggcc ggacaaatca gaaagatttt cccggacgcc gctgttttca ccaccatgtt
     3121 ggaaaagagg cactactata caaagtgaac agtaactcag cgtattttcc ataaggaaat
     3181 acgacatctg tcagggggag taagttgccg tgaagctacc actcttgctt agcgtgatgc
     3241 ttagttttag cctcggccat gccggcttcg gcagcgtcaa attcgattac ctgaaagtca
     3301 ggggcaatat cgctgagtct acacacaagg ctaagtacaa actagaagtt gataggtctt
     3361 ataagccgtt gggcgagttc catcaccagc cggtgtatga gggcgtgcag ttcaatgtct
     3421 ccctggctgc gttttccagg ggcgagagca tcatcatgat gcacgcggag atgcatactg
     3481 acggctccgg cggtcttgat tacagcaaac tgaagcccga gactctcgac ggcattaaat
     3541 tcacctccag agagcagtgc gcgacaccgg cggaagttcc cgacccttat agcaatcctg
     3601 aattccgctt cttacgtgat aagggattca aggctgtcct tcccatctat ctcaggcaat
     3661 attttgccac ttcgccggac ggcgtcgctg aagtcgtgct cacctatggc aagcgcgtgt
     3721 catcgtgtgg cgatgacaca atcacacccg agtttaaggc agaggttgac cgcgaagtcc
     3781 gttcctcgat gaaggtttct atgaagagat aagtggtcac cgggcacccg gcacagccca
     3841 acaactcatt caactcgacg cttgggtagc atgcttttca tcgtgctctt cttcgataat
     3901 atggaatgct tctcgctcgg cgcgggttaa tccgagcatt cggccactga cgcagtctgt
     3961 aaacgggaaa agctgtgctc accgatcaag agctgatggc ccttcacgtc cgggcgttgt
     4021 ttacgcacga cgcccgctcg cgacttttgt ttgttaacga acccggaggc ggagtctccg
     4081 ctcctcgtct gtttctcggt cggacgcggg caggtagtct ctggcggttt cgggcggatc
     4141 ttccagcggc gctcgttgag gaattggaga cactgtgtgc ggacgagccg ccgggcgcgg
     4201 agttccgcag tacacaccgc cacgtcgagg catacgtgcg gctactcgaa acgcacgcgc
     4261 ccgtccacag acagtgggcg gggccggcct atcgctttac tgaatacctt gagccgtcaa
     4321 ggccgctgct cgctatcacg gaaacgaacg ccgagatgtt acggggcggc tttgaggaac
     4381 tgatcgcaga gctgcctgaa tgccagccct tcgtcgccgt cgtagaggat ggcatggcgg
     4441 tgtctgtctg tcgcagtgtt cgcatcactt ccgcggctca cgaggccggt gtggagacgc
     4501 tgcctgattt tcgtggaaag ggatacgcga aggatgcggt ggccgggtgg gcgcgtttag
     4561 tccggtctgt aggcgcgaca cctttgtaca gtacgtcctg ggagaatacc gcctcgcagg
     4621 cgttggcgaa gaagtcgcgt ctggtgcttt acggctccga ttttcacgtc acgtaagtac
     4681 tgacgcactc tacgttaaac ttcatcgccg gcgaactgct cgcggaattc gggtgagcgg
     4741 cggcggagga cgacccagtc gtcgaagatt tcgggctggc ggagccagac ggtgaaccac
     4801 tcggcaatct cggccttctc ggcgcgcttg cgctcgttga ccgattggtt gcgcgcaatc
     4861 atctgcgcgc gctgcttgcc gcgcagcacg gtctcgtgga cgcgtcgctg gccctcccgg
     4921 tcggagcggc gcttgaactc acggcgcagg ttgtcgagac gcttcaggga ggcgacggcc
     4981 tcgtcgaagg tcgagaactt gaggacattg cggaacatgg cgtcgtaggg gtcggccgtg
     5041 cgccagcgcg cgtccagctc caacacctcg gcgtgacgca gctccgctcc ctcgtcggcc
     5101 agcacccgcg cgacgcgagc cgggctttcg accgcgccct cgccaaagcg cgcgcccacc
     5161 gcctcggaga cggcctcaag ctcctgcgcg cccaccgact cgcagtccaa agcctcccag
     5221 acctcgatca tcaagtcgag cttcgtgcgt gacttccact ctgtcttctt catcgccaac
     5281 agagagtaaa ggagtgcttg cggccaagtc cagaggaagt aaaaagtaaa aaggcaaaag
     5341 gcaaaattgg aaggcggaaa cgcatcctca cttttgcctt ttgccttttt actttgcctt
     5401 ctcaggcggc gcgggacgag gcggtcagcg ccgcggtgtt gaatccgatt cttgccctga
     5461 cgcttggccg cgtacatgag ggtgtcggcc gcgcgccatc gtccgcactg cgcgccccgt
     5521 gcctccagat gatctcgggc aagcagtacg ccgcgccgta ctactgggcg cccttagtcc
     5581 tcgtcggcga ctggcgttga cccgcccggc gccgcgggaa gtagtcgtct cgggcggcgc
     5641 gcgttgtaca cggcggcgcg ctggtctaat atcggctcac agacttctgt ttcaaatgaa
     5701 cgtgtgtttc aaatgaacgt gtttcaaatg aacgaacgcg agggcagggt gaaatccgat
     5761 gggtgagcaa ttcgttacgg tgagcaacgc gggggagttg gagcaactct tcgcgcgctc
     5821 gcaccgcgag ccggtgcttc tcttcaagca cagcacgacg tgcccgatca gcgcggcggc
     5881 ctaccagcag atgctcggcg tggagcgcga ggcgtcgctc gtcgtggtgc aaaaagcgcg
     5941 cgacctctcg cgcgagatcg agacacgcac gggcgtgcgc cacgaatcgc cgcaggcgct
     6001 catcctgcgc gacggcgcgg tcgtctggtc ggcctcccac tggaacatca cggccgacgc
     6061 ggtcacgcgc gcgctcggcg agcacgcgga gtcgggagcc aaaagtcagg agccaaaagt
     6121 caggagtcag gagtcagaat aaagacgccg ggatttggca acgaaactgc tcgctgcaat
     6181 gagtggtttt attctgactc ctgactcctg acttttggct cccttcgttt cccatgtccg
     6241 acaccgcacc gcgtcgcggc ccagaggttg agccgcaaga gatcatgcag gatggcgacc
     6301 ggcagttgcg cgtcacctgg gccgacggcc gcgcctgcct ctacggggcg gccgcgttgc
     6361 gccgcgcgtg cccctgcgcg caatgcgtca acgagtggac gggcgagcgc gtgctcgcgc
     6421 ccgaagcggt ctccgacgac ctcgaaatct ccgacctcca gctcgtcggg cgctacgcgc
     6481 tcaacttccg ctggagcgac ggccacgaga ccggcatcta cagcttccgc tacctgcgcg
     6541 agttgtgcga acagagcaaa gagataagtg atgagtgata agtgataagt gataagagaa
     6601 aacaaccgtc attctctttc ttatcactca tcacttatca ctcatcactt caaacctatg
     6661 cccaagaaaa ttctgatagt cgaagacgac gacgttgagc gcgagttggt gcggatgact
     6721 ctggagcgcc agggctacgc ggtcgagacg gccgaggacg gcatcagtgg ctacgagctg
     6781 gccgtgagct accgccccga cctcatcgtc gccgacctcc agatgcccgc ggccgacggc
     6841 gtacacctcg tccgccgcgt gcgcgacacc gccgagatcg ccgccacccc cgtcgtcatc
     6901 gtctccggca tgggcttcgg cgaggccgcc tacacgctca cgctaggggc cgacgcctac
     6961 gttccgaagc cgctcgaccc ggagtcgctc atcgagaccg ttcgccggct tttgtcctga
     7021 ggggcgctta tcggcgataa tgccggcgcg ggcaaattaa ggagtcggag caagggggaa
     7081 gagaagtgac ggaggccgtc ggctgggcaa gctcctgcat cctcgtcctg acgatagcga
     7141 agcaggtcta caagcagtgg aaggaggggt cgagcgaagg cgtctcgcgt tggctcttca
     7201 tcgggcagat ggccgcctcg ctcggcttca cgatctacag ttggctcgtc ggaaactggg
     7261 tcttcgtcgt caccaacgcc gtgatgctcc tgagcggcat cgccggactg ctcatcgtcc
     7321 tccaccaccg ccggcgggag gcgtgaaggc tgttagcgat tagctgttag ctgttagcca
     7381 gaggcaggag ccaaaggctg gccattgttg ataagtaaaa cttcgatttg ctctctgctg
     7441 gctgaaagct aacagctaat cgctaacagc caccagacca cggacaatct ttttatgaac
     7501 gaacgcgatt tcttcgacga acggcaggag acgaagcccg ccaactatag ctgcccacac
     7561 tgccgcgagc gcgacgacta cgaggtgcgc tggctccggc gcacgcgcaa gcatcagccg
     7621 ccgcgcgggg ccaacgagat ggatcgcgcg cgcttccaga agtcgcgcga ctacatggtg
     7681 cgcgtcgatg accagctcgt gtgcaagcgt tgccgccgcc gcttcgacat cccgaacatg
     7741 cagaccgtcg tcttcatcta aggcggcgcg tgcgcgtgcg tcttgtctga ttaacaaacg
     7801 aacgctaaag gagaatcatc ccgccatgga cgaactcatc aaacaggtgg ccgagcgcac
     7861 aggaatcggt gaggacaagg cgcggacggc cgtggacacg gtcgtcggct ttctgaagga
     7921 gcgtctgccg gcgcccatcg ccgggcagct cgacaacgtc atcggcggcg gcggcgctgc
     7981 cagcggcggc gggattgccg atcaggccgg cgacgtgctc gggagtctgg gcggaatgtt
     8041 cggcaagaaa gactaagggc cgcgaccccc gacgggcgcg tgaatctgaa gcgggcgggc
     8101 tgccagagac ggtagccggc ccgctttctc tttgtgggaa cccacagccc cgtggggcgg
     8161 ctcgcaaccc gtcgcgacgt tggcccgctc gtatcgacat tcagtgcgcg ggtctaccat
     8221 cgtctcggtt cgacaggatt tccctgtcac gggagaaaac aaaaatgctc tggacaatgc
     8281 gactcgtcat cttcgtcttc gcgctcgtct tcggcgccgg ctgctggtac gtcttcgcgc
     8341 agacgccgtc cgcgcagacg accaacacga acgtcaacgg caacatgaac gccaacaacg
     8401 ccagcgcgga agacgccgcc gccgactgga cgcccgtccc aacgaacacg aacaatttgc
     8461 ccgacgacca gagcgcggag gcgcacgagg aagaggcggc gcccgcgctc gaatgggccg
     8521 agccgaatcc ggcttacgga cacgtcgtcg cgccgcgcgt cacgctgcgg agcgagtcga
     8581 acgcatcgtc caacatcatc gccacgctcg acgtggttga gaacgagtcg gtcgagatca
     8641 tggactcgac gcgcgacttc ctgcgtgtga agtttcccgc caacacggaa atggagggcg
     8701 ggacgcggca gcaggattat gacggttggg tcgagtgggg cgcggtcgtg ccgtacacaa
     8761 acgcgctcgt cgtcgagacg gagagcggcg aagtgatcgg tcgtgtggcg ctcgactacg
     8821 gaatctccac cgcggccttc tcgcctgacg gctcgcgcgc cgtgttctac ggcgcgtcgg
     8881 gtggggatgg tcatggcgag tctcagtccc tggcctacga gttcgacacg gagagttaca
     8941 aaatcctgcg aaccctcaag acctcgacca gcggcggctt cgcctccatt ctttacgacg
     9001 acggcggcga ccttcgccca cttctccaga cacacctcac gagcggcgac agcatgcgga
     9061 agatgctgca acccttccgc gtcaaaggcg atctgttcga gcgctccggc gacacgatac
     9121 gccccggcgc cgacggcccg ctcctgttct cgcgcgacgg cgcgaccggc ttcgacatcc
     9181 acagtgctga cgaagcgccc ggcgcgatca gcgttgatgt catcgacgtg aagagtttca
     9241 ccgtccgcaa cagcttcccg gtcgagggca cggccagcga accgtggtct tacgaatatg
     9301 ccgttagccc cgatggcgcg gagttcttct acagggagag cgagggaagc atccgggtcg
     9361 tcgatacgcg aacgggcctg cgcgtgcgcg agacaaggcc gcgcgtggcg gaggacttcc
     9421 gcggctacct cgccaacgac agcgtcgtgg gcgactcgct gctcatcagg tattggcagg
     9481 agagcgaaag cgaagagggc ggggagttac gctccttctg gctgaaggcc gacggcgaga
     9541 cgacgcccgc cgagcgaggc atcaacaacg tcgtcgaggc gggcggcgcg ctctacgccg
     9601 tcaacgacgc gggcacgcgc ctgttcaaac tcgacgccga cggccgcatc acggagcgac
     9661 tcaagattgc ccgccccgag ttgaagcacc ggccgaaggg gtcggaagaa cacaccatca
     9721 tgggcctcta cgccagcccc gacggcaagc acatcgtcat cttctttggg ataccggacg
     9781 gctgctgatg aagttgggga aaggggaagg ggtaaagggg aaagggaggg agccacgagt
     9841 cacctgggtt tatcaggttt cttttcgcgc tcctcctttt ccttaatcgc tttgatttga
     9901 tttgagagtt ggcggcttag ggaggaaaca aattctcgta attcgtcgtg tttagattga
     9961 ccgagatagc cgacctctaa cgccacccgc aggagacttc gcacttcacc ggcagaccct
    10021 ttagcgatat tgaggaacaa caagtattct ttgcgcgaag accgctcaaa gccctcggcg
    10081 atgttagtcg gaatcgagac tgcggcacgt cgaatctgat cgcgcaaacc aaaatctctg
    10141 ctgaaagcgc cggcgct
//
LOCUS       DASVES010000267         6812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_399164, whole genome shotgun sequence.
ACCESSION   DASVES010000267 DASVES010000000
VERSION     DASVES010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399164"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3098)
                     /locus_tag="VF240_10735"
     CDS             complement(<1..3098)
                     /locus_tag="VF240_10735"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX7175724.1"
                     /translation="MSQQRNTTDYSEIIAENFGANATYVEGLLSRFRSDPSLVDEAWR
                     AYFTEMLGDASPAAAPAGPNGGATATARTDAASATTASDAWPTPEQATRTTASAPAAQ
                     PQQSAAQQPQQTPAPQPAQAPAQDGAKGTAAAAQTPAAPQTASQPAPSAPTAAAPPPA
                     PEAQPIRGGALKIVENMETSLTVPTATSNRQIPVKVLEENRIIVNQFLKERGRGKTSY
                     THFIAYAILRALDAFPQMNDGFAVVDGHPVRLRRSSVNLGVAVDLQKKDGTRTLLVPN
                     VKNAGALRFSEFVAAYDDVIKRAREGKLAVADFQETTISLTNPGTIGTVSSNPRLMAG
                     QSVIIATGAIEHPAEFHAMTPESLSQLGISKTFNISSTYDHRVIQGAESGGFLARVHE
                     LLVGKDNFYENIFRDLGIAYQPMRWSVDRNPALLGADRTREQIEKQARVLELVNAYRV
                     RGHLIADIDPLNALPVQYHPELDIETYGLTIWDLDREFITGGLAGRDIAPLRVIIDIL
                     RRAYCGKVGTEYRHIQSKEQKVWIRERIREEFVEPQPLPAEIKRALLARLTRAEQFER
                     FLHTKYLGQKRFSLEGCDTIIPLLDQLVERASERGVEEIALGMAHRGRLNVLANVVGN
                     FSERIFTAFEGSVHPDFPADEGDVKYHQGAVGVRETAAGRKVRLTISPNPSHLEFVDP
                     VVEGMVRARQDEWQLAGSAREEVIDRALPVLLHGDAAFAGQGIVMETLQLADLKGYRT
                     GGTIHIIINNQIGFTTSPAAGRSSIYSTDVARMTQIPIFHINGDDPEAAFRTLRIALD
                     YRQEFNKDVVLDVVGFRRLGHNEGDEPSYTQPLMYARVKAHPGVRAKYAEQLVREEVL
                     TQEEVEGLVKEAVAYYEGIQARAKEIVKSKPPKQELPPQPDEEDGSVVVETAVGTEVV
                     REVAHKIAVVPEGFSVNPKMVSQLGRRAKMGDGQVPMDWAFAELVAFGSLGLEGVRVR
                     LSGQDSGRGTFSQRHAILYDTQTGKAWSPLSDMTARGGDGRSLVEVFDSSLSEQGVMG
                     FE"
     gene            3427..3846
                     /locus_tag="VF240_10740"
     CDS             3427..3846
                     /locus_tag="VF240_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175725.1"
                     /translation="MTKNKSSKSGARPHESDARSRESDAGARQSRRRFAKSVAAAIVA
                     APVAAARAQTPAAPRESKAPPKAQPTPTPRAQTPQPPSPVAKAYAEVARARFGSQVTD
                     EEFKRIERDLEGNVRTADRLRASKLENWDEPDFVFEA"
     gene            3942..5486
                     /locus_tag="VF240_10745"
     CDS             3942..5486
                     /locus_tag="VF240_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7175726.1"
                     /translation="MKHPITLLLSAVAMCLPSLVSGRAVDSVRDYRRANEHRILREFV
                     ELLSLPNVASDRENIRRNAAHILAMMERRGLKPRLLEAKDPAAPPVVYGELLTSGAAR
                     TLVFYAHYDGQPTDPQKWTGTQPWQPTLRSAATESGGRIIPLPKEGEAINPEWRLYAR
                     SASDDKAGVVAILTGLDALRATGARPVVNLKFFFEGEEEAGSPHLAEILSLNRELLRA
                     DGWIVCDGPVHQSGRKQVVFGVRGDTNVDVTVYGARRPLHSGHYGNWSPNPAMTLARL
                     LTSMKDERGRVTIEGWYDDVEPLGEAERRALAEAPAFDDELRRQLGLARTEQAAPSLL
                     EAINLPSLNINGMTSAETGALARNVIPTTATAALDLRLVRGNDYRRQFERLAAHVRRQ
                     GFYVIDREPTDDERLRHPLIAKLVHRTGGYNAQRTRMDLPLSLAAIAAVQSVAAEPVV
                     RLPTLGGSLPLSIFNEVLGVEALITVPIANYDNNQHAEDENIRLQNLWDGIEIMAALM
                     TLKSDK"
     gene            5622..>6812
                     /locus_tag="VF240_10750"
     CDS             5622..>6812
                     /locus_tag="VF240_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7175727.1"
                     /translation="MLPDEVFFSTVTELAPRLRRREISPVELAEGYLQRLESTGARLG
                     AVATVMRERALREARSAETEIRRGRYRGPLHGVPYGVKDLLATRDAPTTWGAPPFRDQ
                     RFDYDAAVVEKLRAAGAVLLAKLSMIELAGGLGYNRADASLTGACRTPWNTDFWSGGS
                     SSGPGAATAAGLVAFAIGSETSGSIITPAAFCGVSGLRPTYGAVSRRGAMALCWTLDK
                     LGPMCRSAADCGLVLAAITGRDPLDASSKDFKFEFPSRRKRARLARPIAVLKDSFEKA
                     QPEVRDNFLRSLDVLKRFATLEMNVPLPDFPYGPAVGTIVDAEGASAFRDLIESGRVK
                     EMANQNDRVGGYSALLVTAVDYLHAMRLRVPMRRAWGEFFRKYSLLVAPSRATVAYPV
                     DKNFE"
BASE COUNT         1114 a         2338 c         2199 g         1161 t
ORIGIN      
        1 tactcgaagc ccatcacgcc ctgctcggag agcgagctgt cgaagacctc gacgagcgag
       61 cgcccgtcgc caccacgcgc cgtcatgtcg gagagcggcg accacgcctt gccggtctgc
      121 gtgtcgtaga ggatggcgtg acgctggctg aaggtgccgc gtcccgaatc ctgcccggag
      181 agacgcacgc gcacgccttc gaggccgagc gagccgaagg cgacgagttc ggcgaaggcc
      241 caatccatcg gcacttggcc gtctcccatc ttcgcgcgcc gtccgagttg gctgaccatc
      301 ttcgggttga cgctgaagcc ctcggggacg acggcgatct tgtgcgccac ctcgcggacg
      361 acttccgtcc cgacggccgt ctcgaccacc acggagccgt cctcctcgtc gggctgcggc
      421 ggcaactcct gcttcggcgg cttgctctta acgatctcct tggcgcgcgc ctgtatgcct
      481 tcgtagtagg cgaccgcttc cttgaccaat ccctcgacct cttcctgcgt cagcacctcc
      541 tcacgcacga gttgctcggc gtacttggcg cgcacgcccg gatgggcctt gacgcgcgcg
      601 tacatcaggg gctgcgtgta gctcggctcg tcgccctcgt tgtgtccgag gcggcggaag
      661 ccgaccacgt ccagaacgac gtccttgttg aactcctgac ggtagtcgag cgcgatgcgc
      721 agcgtgcgga aggcggcctc ggggtcgtcg ccgttgatgt ggaagatggg aatctgcgtc
      781 atgcgcgcca cgtccgtcga gtagatggac gagcggcccg ccgcgggcga ggtggtaaac
      841 ccgatctggt tgttgatgat gatgtggatg gtgccgcccg tgcgatagcc cttgaggtcg
      901 gcgagctgca aggtctccat cacgataccc tgcccggcga aggccgcgtc gccgtgaagc
      961 aggacgggca gggcgcggtc gatgacctct tcgcgcgccg agccggcgag ctgccactcg
     1021 tcctgccggg cgcgcaccat gccctcgacc acgggatcga cgaactccag atggctcggg
     1081 ttgggcgaga tggtcaggcg caccttgcgg ccggcggccg tctcgcgcac gcccacggcg
     1141 ccctgatgat acttcacgtc gccctcatcc gcgggaaagt ccgggtggac gctcccctcg
     1201 aacgcggtga agatgcgctc ggagaaattg ccgacgacat tggcgagcac gttcagacgg
     1261 ccgcggtgag ccatccccaa cgcgatctct tcgacgccgc gctccgaggc gcgctcgacg
     1321 agctggtcga gcagcgggat gatcgtgtcg cagccttcga gtgagaaacg cttctggccg
     1381 agatacttcg tgtgcaggaa acgctcgaac tgttcggcgc gtgtcaggcg cgcgagcagg
     1441 gcacgcttga tctcggccgg caacggctgc ggctcgacga actcctcgcg gatgcgctcg
     1501 cgtatccaga ccttctgctc cttgctctgg atgtgccggt actcggtgcc caccttgccg
     1561 cagtaagcgc gccgcaggat gtcgatgatc acgcgcaagg gggcgatgtc tcggccggcc
     1621 agaccgccgg tgatgaactc gcggtcgagg tcccagatgg tcagcccgta agtctcgatg
     1681 tcgagttcgg ggtgatactg gacgggcagg gcgtttaagg ggtcgatgtc ggcgatgagg
     1741 tggccgcgca cgcgataggc gttgacgagt tcgagcacgc gcgcctgttt ctcgatctgc
     1801 tcgcgcgtgc ggtcagctcc gagcagcgcc gggttgcgat ccacagacca gcgcatcggc
     1861 tggtaggcaa tcccgaggtc gcggaagatg ttctcgtaga aattgtcctt gccgaccaga
     1921 agctcgtgga cgcgcgcgag gaagccgccc gactcggcgc cctgaatgac gcggtgatcg
     1981 taggtcgagg agatgttgaa ggtcttgctg atgccgagct gcgaaagcga ctcgggcgtc
     2041 atcgcgtgaa actcggccgg gtgctcgatg gcccccgtgg cgataatcac cgactgcccc
     2101 gccatcagtc gcgggttcga agagacggtg ccgatggtgc cggggttcgt caaagaaatc
     2161 gtcgtctcct gaaagtcggc gacggcgagc ttgccctcgc gcgcgcgctt gatgacatcg
     2221 tcgtaagcag cgacgaattc tgaaaagcgc agcgcgccgg cgttcttgac gttcggaacg
     2281 agcagcgtgc gcgtcccgtc tttcttctgg agatcgacgg cgacgccgag gttgaccgac
     2341 gagcggcgca ggcgcacagg atgaccgtcc acgaccgcga agccgtcgtt catctgcggg
     2401 aaggcgtcga gggcgcggag gatggcgtag gcgatgaagt gcgtgtagga ggtcttgccg
     2461 cgcccgcgct ccttgaggaa ctggttgacg atgatgcggt tctcttccag aaccttgacg
     2521 ggaatctgcc ggttcgaggt cgccgtcggc accgtcaaag aggtctccat gttctcgacg
     2581 atcttgagcg cgccgccgcg tatcggttgg gcctccggcg cgggtggcgg cgcggcagcc
     2641 gtgggcgcgg acggtgcggg ctgagaagcg gtttgcggag cggcgggcgt ctgcgctgcg
     2701 gcggccgtcc ctttcgcgcc gtcctgcgcg ggagcttgcg cgggctgtgg cgcgggcgtc
     2761 tgctgcggtt gctgcgcggc ggactgttga ggttgtgcgg ccggggcgct tgcggtcgtg
     2821 cgcgtcgcct gctcgggggt cggccatgcg tccgacgctg tcgtggcgct ggcggcgtcg
     2881 gtgcgcgccg tcgcagttgc gccgccgttg ggacccgccg gcgcggcggc cggcgatgcg
     2941 tcgccgagca tttcggtgaa gtaggcgcgc cacgcttcgt ccacgagcga cgggtcgctg
     3001 cggaagcggc tcaaaaggcc ctcgacgtag gtggcgttcg cgccgaagtt ttcggcgatg
     3061 atctccgaat agtctgtcgt gttgcgttgc tggctcacga gttttagcac ctcacgcccc
     3121 gacgcctgcg gccctcggcc tcggcctcgc gcacggagaa agttttgtct gactcgaacc
     3181 ggcggaattt ccaatagcct accaccatcc gcgcgcgttt gcacactgat taccgcgcgc
     3241 ctgaatcgcc cacgccgcgg cccgtttccg atgatatact gcgcctcgcg ccgccggcgc
     3301 taacttactt ttttcgtagc ttcgcccttc atttatcgac gagtcgaaac cgggagacac
     3361 gaccgtgcgg aaactcttct tcgccctcgc gcccgcgctc ccgcacggaa agggagaacc
     3421 aatcagatga cgaagaacaa gagcagcaag tcgggcgcac gcccacacga gtcggacgca
     3481 cgctcacgcg agtcggacgc gggcgcgcgc cagtcgcggc gacgcttcgc gaagtcggtg
     3541 gcggcggcaa tcgtcgccgc gccggtcgcg gccgcgcgcg cgcagacgcc cgccgcgccg
     3601 cgcgagtcga aagcgccgcc caaagcgcag cccacgccca cgccgcgcgc acaaacgccc
     3661 cagccgccct cgcccgtcgc caaagcctac gccgaggtgg cccgcgcacg cttcggctca
     3721 caggttacgg acgaagaatt caagcgcatc gaacgcgacc tcgaaggcaa cgtccgcacg
     3781 gccgaccgac tacgcgcctc caagctcgaa aactgggacg agccggactt cgtctttgag
     3841 gcgtagagaa aagctgtcag ctattagcga ttagctgtca gccaaacccg gttcactctt
     3901 ttaaagctac ttgctaacag ctaatcgcta atagcttagt tatgaaacat ccgatcacac
     3961 tcctgttatc cgctgtcgcg atgtgcctgc cgtcgctggt gagcgggcgc gccgtcgatt
     4021 cggtgagaga ttaccggcgc gccaacgagc atcgcatcct ccgcgagttc gtcgagctgc
     4081 tctcgctccc gaacgtcgcc tcggatcgtg agaacatccg ccgcaacgcc gcgcacatcc
     4141 tcgcgatgat ggaacgtcgc ggtctgaagc cgcgtctgct cgaagccaaa gaccccgccg
     4201 cgccgcccgt cgtctacggc gaactcctga cgagcggcgc cgcgcgcacg ctcgtcttct
     4261 acgcgcatta cgacgggcag cccacagacc cgcaaaagtg gacgggcaca cagccgtggc
     4321 aacccacgct gcgctcggcg gccacggagt ccggcggccg aatcattccg ctgccgaaag
     4381 agggcgaggc gatcaacccc gagtggcgtc tctacgcgcg ctccgcctcg gacgacaaag
     4441 ccggcgtcgt cgccatcctc acgggtctcg acgcgctcag ggccacgggc gcgcggcccg
     4501 tcgtgaacct caaattcttc ttcgagggcg aggaggaggc cggctccccg cacctcgccg
     4561 agatactctc gctcaaccgc gagctgttgc gcgccgacgg ctggatcgtg tgcgacggcc
     4621 ccgtccacca gtcgggacgc aagcaggtcg tcttcggcgt gcgcggcgac acgaacgttg
     4681 acgtgaccgt ctacggcgcg cgccgcccgc tgcacagcgg ccactacggc aactggtcgc
     4741 caaacccggc gatgacgctc gcgcgcctgc tcacctcgat gaaggacgag cggggccgcg
     4801 tcaccatcga gggctggtac gacgacgtgg agccgctggg cgaggccgag cgccgcgcgc
     4861 tcgccgaggc tcccgccttc gacgatgagc tgcgacgaca acttggactc gcccgcaccg
     4921 aacaagcggc cccttccttg ctcgaagcca tcaacctgcc gtcgctgaat atcaacggca
     4981 tgacgagcgc cgagacgggc gcgctcgcgc gcaacgtcat ccccaccacc gccacggccg
     5041 ccctcgacct ccggctcgtg cgcggcaacg actaccgacg ccagttcgag cgtctcgccg
     5101 cgcacgtccg ccggcagggc ttttacgtca tcgaccgcga gccgacggac gatgagcgcc
     5161 tgcggcaccc gctcatcgcg aagctcgtcc atcgcaccgg cggctacaac gcgcaacgca
     5221 cgcgtatgga cttgccgctc tcgctcgcgg ccatcgcggc cgtccaatcc gtcgcggccg
     5281 agccggtcgt gcggctcccg acgctgggcg gcagcctccc gctctccatc ttcaacgagg
     5341 tgctcggcgt cgaggcgctc atcaccgtcc ccatcgccaa ctacgacaac aaccagcacg
     5401 ccgaggacga gaacatccgc ctccagaacc tgtgggacgg catcgagatt atggcggcgt
     5461 tgatgacttt gaagagtgat aagtgatgag tgataagtga tgagtgataa gtgataagaa
     5521 agagcggagc tgttagcttt tggcttttag ctaagagtta atcgctaaca gcctcttatc
     5581 acttatcact catcacttat cacttcctct tgaggatact gatgctccct gacgaagtct
     5641 tcttctccac cgtcaccgaa ctcgccccgc gcttgcgccg ccgcgaaatc tcccccgtcg
     5701 agttggccga gggttatcta caaaggctag aatcaacggg cgcccgcctc ggcgccgtcg
     5761 ccaccgtcat gcgcgagcgc gcccttcgcg aggcgcgctc ggccgagacg gagattcgcc
     5821 ggggccgata ccgcggcccg ctccacggcg tcccctacgg cgtcaaagac ctcctcgcca
     5881 cgcgcgacgc gccgaccacc tggggcgctc cgccctttcg tgaccagcgc ttcgactacg
     5941 acgccgctgt cgtcgaaaaa ctaagagccg ccggcgccgt gctcctcgcc aagctctcga
     6001 tgatcgaact cgccgggggc ctcggctaca accgcgccga cgccagcctc accggcgcct
     6061 gtcgcacgcc ctggaacaca gacttctgga gcggcggctc ctcctcgggg ccgggcgccg
     6121 ccacggccgc cggcctcgtc gccttcgcca tcggctccga gacctccggc tccatcatca
     6181 cgcccgccgc cttctgtggc gtctccggcc ttcggcccac ttacggcgcc gtctcccgcc
     6241 gcggcgcgat ggcgctgtgc tggacgctcg acaagctcgg ccccatgtgc cgttcggccg
     6301 ccgactgcgg cctcgtgctc gccgccatca cgggccgcga cccgctcgac gcgtcgtcga
     6361 aagatttcaa gttcgagttt ccgtcccgcc gaaaacgcgc gcgcctcgcg aggcccatcg
     6421 ccgtgctcaa agactccttc gagaaggcac agccagaggt gcgcgacaac ttcctgcgct
     6481 cgctcgacgt gcttaagcgt ttcgccacac tcgaaatgaa cgtgccgctg ccggacttcc
     6541 cctacggccc ggccgtcggc acgattgttg acgccgaggg cgcgagcgcc ttccgcgacc
     6601 tcatcgagtc ggggcgcgtg aaagagatgg ctaaccagaa cgaccgcgtc ggcggctatt
     6661 cggcgctgct cgtcacggcg gtcgattacc tgcacgcgat gcgcctgcgc gtgcccatgc
     6721 ggcgggcgtg gggcgagttc ttccgtaagt attcgctgct cgtcgcgccc tcacgcgcga
     6781 cggtcgccta ccccgtcgat aaaaacttcg ag
//
LOCUS       DASVES010000268         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_633922, whole genome shotgun sequence.
ACCESSION   DASVES010000268 DASVES010000000
VERSION     DASVES010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633922"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VF240_10755"
     CDS             <1..1193
                     /locus_tag="VF240_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175728.1"
                     /translation="CHVTDGADFRYTVTYQFEQHENVLPPVRRWSREVFEGRILPAAE
                     AMDAANLFSPYMVFRDNRDPAKGIDSFIATPRFATGYVPVVRNRPALLIETHMLKPHR
                     LRVRGTYDILRAALEEVNKNPDELLRAAREADALVVQSGGRYDPAGRVALSVELTDKP
                     ATKLLKGFEFRTELSDISGAVRVVWGDKPLDLNVPFYTDARAARSVSPPLFYVVPPQW
                     TAAAEVLRAHGLKLQRLAAPLSVEVESYRFRDVKWSPNSFEGRTLVSFKAEAVREQRT
                     YPANSVVVSMSQPAARAALQLLEPEAPDSLATWGFFAPVFEQKEYGEDYILEKLAREM
                     LAKDADLRREFETRVATDPKFAASANERLRFFHERSPYFDRMMNIYPVGRITSPLSAR
                     LTDF"
     gene            complement(1321..1467)
                     /locus_tag="VF240_10760"
     CDS             complement(1321..1467)
                     /locus_tag="VF240_10760"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX7175729.1"
                     /translation="MLWTILVVLLILWLIGWGTGAVGSLIHIILVIALVLLLIQLLTG
                     RRTV"
     gene            2069..>3184
                     /locus_tag="VF240_10765"
     CDS             2069..>3184
                     /locus_tag="VF240_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7175730.1"
                     /translation="MSRSRLVTPTRKKSLAVGLLIILAGLVVVGISYFTSPAPSTARN
                     INYTQLRELAAAGAVAHVVIEGEAVTVTMSDGSAARAVVVNQAAQQEVAAAFANNNAQ
                     VEYRVLQPGFWAVAINWMFPVLMLLAFGLIGWRLYASVGGTGDFQLNDVSGNQPVTFD
                     MVAGVDEAKSELAETIEFLRDPVKFGRLGGRAPRGVLLSGPPGTGKTLLARAAATEAK
                     VPFLSVSGSSFQEKFAGLGAARVRRLFARARKLSPCVIFIDEIDALGRRRGRGQDSAS
                     ADQDQTLNQLLIEMDGFEQMNGVVVIASTNRPDILDTALTRPGRFDREITVNLADLRG
                     RHAILKVHAEKLTLEETLELEWIARGTPGFSGADLANL"
BASE COUNT          607 a         1024 c          920 g          633 t
ORIGIN      
        1 actgtcacgt tacggacggc gctgacttcc gctacaccgt cacctatcag ttcgagcaac
       61 acgaaaacgt cctgccgccc gtccgccgtt ggtcgcgcga ggtcttcgag gggcgcattc
      121 tcccggcggc cgaggcgatg gacgctgcca acctcttctc gccctacatg gtttttcgcg
      181 acaaccgcga ccccgcgaag ggaatcgaca gcttcatcgc cacgccccgt ttcgccaccg
      241 gctacgtgcc cgtcgtccgc aaccgccccg cgctcctcat cgagacgcac atgctcaagc
      301 cgcaccgcct gcgcgtccgc ggaacctacg acattctgag ggcggcgctt gaagaggtga
      361 acaagaaccc cgacgaactt ctccgcgccg cccgcgaggc cgacgcgctg gtcgtccaaa
      421 gtggcggacg atacgacccg gccggaaggg tggcgctgag cgttgagtta acggataagc
      481 cggcgacgaa gctgcttaag ggcttcgagt ttcgcacgga gttgagcgac atctccgggg
      541 ctgttcgcgt cgtgtggggg gacaagccac tcgacttgaa cgtcccgttc tacactgatg
      601 cgcgcgccgc ccgctccgtc tcgccgccgc tcttctacgt cgtcccgccg caatggacgg
      661 ccgccgccga agtactcagg gcgcacggcc tcaagttgca gcggctcgcc gccccactct
      721 ccgtcgaggt cgagagctat cggttcagag atgtgaaatg gtcgccaaat tctttcgagg
      781 ggcgcacgct cgtctctttc aaggccgaag ctgtgcgtga gcagcggact tacccggcaa
      841 actcggtggt cgtgtcgatg tcgcagcccg ccgcgcgcgc cgcactccaa ctgctcgaac
      901 cggaagcgcc cgactcgctt gccacttggg ggttcttcgc ccccgtcttc gaacagaaag
      961 agtacggcga agactatatc ctcgaaaaac tagcccgcga gatgcttgcg aaggatgctg
     1021 atctgcgtcg agagttcgag acgcgagtag ccaccgaccc gaagtttgcc gcgagcgcca
     1081 acgaacgcct gcgatttttt cacgagcgct cgccatactt cgaccggatg atgaatattt
     1141 atcccgttgg tcggataacg tccccgctgt cggcgcggct cactgacttc tgaagttttt
     1201 cgggacgggc cgtcccttcg atcaagagtg agaagtgccg aacaagaaaa gaaggggagg
     1261 tgaacagatg cctgttcgcc tccccttccg tgcttgagct attcgtatgt ttcgtctcgc
     1321 ttagaccgtg cggcgtccag ttagcagctg gatgagcaga agcacgagcg cgataacgag
     1381 aatgatgtga atcaagctgc cgaccgcgcc ggtgccccag ccgattaacc acaagatcag
     1441 aagcaccacc agaatcgtcc acaacatatt cgccttctcc tctccgtttt tgagcgtcgt
     1501 agtttaaact ttcgggtcgt ctttaaccca actgcatttg ttgatgagca acgtctgtgc
     1561 cagccgcggg tagctcgact cattacggat taagctcttg atttccaaca attaagttgt
     1621 ggggtcgtct ggagttgagc agccgctttg tattgaaacg tggcgcatgc gccgatgata
     1681 tttgaccatt gatgttctaa tacagggcaa atgaaaacgt ggaacgaccg gcgggttgaa
     1741 gagtctacgg caacctgcgg ctcggctcac acatctgagc tgcgtttttg tgggaagaag
     1801 ttccgtaagt tattttacag tcgagtagaa ttaaaaccca ctagactttc caagtgcggg
     1861 gctggtaaat tgtataaagc gttccccgca ttaccctcgg aggggggacg cgctcacaaa
     1921 ggtcgcctcg aaaaactctt cggcgtgccc ttcagataag ccccagcggc tcctccgttt
     1981 cggaactctt tagtaaaatc cgcactcaga aagcaaccgc ggtagttccc ccgaacccct
     2041 cgccgcgttg cccgggagat gttttcgtat gtcgcgctca cgcctcgtca cccccactcg
     2101 aaaaaagagt ctcgccgtcg gcctgttaat cattctcgcg ggccttgtcg tcgtcgggat
     2161 tagttatttc acctccccgg cgccttccac cgcacgcaac atcaattaca cgcaactgcg
     2221 tgagcttgcc gcagccggcg ccgtagcaca cgtcgtcatc gaaggcgagg ccgtcacggt
     2281 gacgatgtcc gacggcagcg cggcgcgggc cgtcgtcgtc aatcaggccg cacagcagga
     2341 ggtagccgcc gccttcgcca acaacaatgc acaggtcgag taccgcgtgc ttcagccggg
     2401 cttctgggcg gtcgcaatca actggatgtt ccccgtcctc atgctcctcg ccttcggcct
     2461 catcggctgg cggctctacg cgagtgtcgg cggcacaggt gattttcaac tgaacgatgt
     2521 gagcggcaac cagccggtga cattcgatat ggtcgcgggc gtggacgagg cgaagtcgga
     2581 gctggccgag acaatcgagt tcctgcgcga ccctgtgaag ttcggcaggc tcggcgggcg
     2641 cgccccgcgc ggcgtattgc tctccggccc gccgggcacc ggcaagaccc tgcttgcgcg
     2701 cgccgcggcc accgaagcca aagtcccttt cctctccgtc tcggggtcga gcttccagga
     2761 gaagttcgcg ggacttgggg ccgcgcgggt gcgccgactc ttcgcgcgcg cgcgtaagct
     2821 ctcgccctgc gtcatcttca tcgatgaaat cgacgccctc ggtcgccggc gtggtcgcgg
     2881 gcaggactcg gcctcggccg accaggatca gaccctcaac cagcttctca tcgagatgga
     2941 cggcttcgag cagatgaacg gtgtcgtggt catcgcctcc acgaaccggc ctgacattct
     3001 cgacacggcc ctcacgcggc ccggtcgctt cgaccgtgag atcaccgtca acctcgccga
     3061 cctccgcggc cggcacgcca tcctcaaagt ccacgctgag aagctgacgc tcgaagagac
     3121 gcttgagctg gaatggattg ctcgcggcac ccccggcttc tccggcgccg acctcgccaa
     3181 tctg
//
LOCUS       DASVES010000269        16957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_884197, whole genome shotgun sequence.
ACCESSION   DASVES010000269 DASVES010000000
VERSION     DASVES010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16957
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884197"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF240_10770"
     CDS             <1..634
                     /locus_tag="VF240_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175731.1"
                     /translation="QRMQIIAVRQRDTRMVGCTGDAQIIISNSDLEDLIVSVLEACDS
                     PADVRTLRSLVMSRLPVMDIYLVPLGGDDAEDGHRGFDPVDGRENPEQVLLRREAESG
                     RDGLVDRFMEGLSASVRGKTKQYDRMLGVLWHCYLNPVHVTQLEVAAMLGVSDSLVTE
                     YRRRIERELRALAIAGVAEARAFEAALRERVSRLPGCSPPARSSRLQTVA"
     gene            complement(886..1140)
                     /locus_tag="VF240_10775"
     CDS             complement(886..1140)
                     /locus_tag="VF240_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175732.1"
                     /translation="MSIVRRCHTSSLPPLSMIELRYTGVMRQSITLTLSAYWEKRLTA
                     AQKRFTRACESMERVRKLSARVPEVQTNVAAEGGRQVNVA"
     gene            complement(1277..1552)
                     /locus_tag="VF240_10780"
     CDS             complement(1277..1552)
                     /locus_tag="VF240_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175733.1"
                     /translation="MNLHMGFGFQIAGEFAERRVRRLDYFVAQRRECLGSQRGRVAPG
                     VRLRREAQPRAVLVYEAGDGAPSDIEEFGHFVQSVIVMFISESNLLS"
     gene            1660..2184
                     /locus_tag="VF240_10785"
     CDS             1660..2184
                     /locus_tag="VF240_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7175734.1"
                     /translation="MESIDRRKLVFTDETGFHLAFTRAFGRAPRGQRVVGHVPFKRGP
                     NHSLIGSLGLRGVVASLLLTGSVDHLVFDAFVNELLLPRLRAGDVLLLDNLPAHKASA
                     VEESAARARAQVSWLPAYSPDFSPNENCWLKIKTFVRGQQPRTAPDLNEALSAAIRAV
                     CKQDIAAWFKHCGY"
     gene            2514..3473
                     /locus_tag="VF240_10790"
     CDS             2514..3473
                     /locus_tag="VF240_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175735.1"
                     /translation="MTRLRFPFRVLGLAILTLAFTTAAQAQATRTFVSGVGNDADPCS
                     RTAPCRTFSGALIKTFINGEINALDPGGYGTLNVTKSITVDGTNGSGYGSILASGTTG
                     VIVNIAPGNVNDPHRSVRLRNLSINGTGPSGAVGTRTGTDGVRMIAGSSLIIDNTVIN
                     DFSQEGVEVAGGVEGNQITVVINNTVIRNCNGTGIRLATGAGSIGAMLSDVRVHTCAT
                     GLLGANRTRINVKNGVFSQNTTGLNQTGTDNITNVEDVFVTIATTGIQSSAGNITRVS
                     NSVVTQTLTGLNPNGGTLHSMSGNAVFGNTADGAFNSGPTPKS"
     gene            complement(3743..5014)
                     /locus_tag="VF240_10795"
     CDS             complement(3743..5014)
                     /locus_tag="VF240_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7175736.1"
                     /translation="MKFTNAFKLVFVIVLIVFCHAVGRAQVTAIKAGKLVDPEAGTVT
                     GNQIILVEGQTIKAVGPDVKIPAGALVIDLSNRTVLPGLFDAHTHLCMTTRRGRDGGN
                     YYFTTLLDSNGYRAIQGVANARSMLETGFTTVRDIGNAGNYADTDLRRSIDEGLVPGP
                     TIINAGRIIAPFGGQFQMQPDKPGLGNPEYFYADTRDELKKAIRENIHYGARVIKLVV
                     DDQRYIYSVEDIRFVIEEAARAGLKVAAHCWTEAGARNAAEAGVASIEHGVAMSNETL
                     VIAKRNGVALVPTPFTETDAREFRTAGANKQVNERWFIDPVKRAHQIGVTLVFGPDVI
                     FTTKEYSRGRLSIETIDNWVEAGIPARVILQALTTNAARLLGVEKERGALRVGMKADI
                     IATAANPLDNLDTLKAVTFVMKNGQIFKENK"
     gene            5348..6211
                     /locus_tag="VF240_10800"
     CDS             5348..6211
                     /locus_tag="VF240_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175737.1"
                     /translation="MPDNQQNTDPTTPSEPETEAQAPAPVSATTQAALDSPNTVTVNP
                     GAQTFPTIGAALASITDNRLQKQYELIVGPGTYNEKVTMKPFVTIQGAGQDVTIITQP
                     AASDAFSGGTVIAASNCTLANVTVSSLGGHWGSYSTALSCSGVTGFSCDASTLLADDQ
                     NQAGINMSTLNASLNTIEPASQIILSYCTIICNAQSNQSGAVSVGIAQNSTAELLDTK
                     IVATGGSQSFGVITARGSSVTLDDCYIQGSSFALYDSDSSSPITANNCQINGNISNGV
                     TVNNNPPPTSE"
     gene            6735..7643
                     /locus_tag="VF240_10805"
     CDS             6735..7643
                     /locus_tag="VF240_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7175738.1"
                     /translation="MGAEIWMASMQAEGDHSVMRVMAGDADDLRARLADAVERLGYHV
                     ISDQAALVARRGNRRLGRSGCSFDLRDYATELIVTLKPKSAGVTLATFNFTVRNYYYW
                     TGGDKRTLECEADALAALAVSQQWLTACTACGTETTDDSRFCRRCGAPLALEEPAELE
                     ALRLTAGTRAGYKNIAWGLLVLVSILIPVVILLTLGASEKAFNAAAIIAASGGGSALF
                     MLLAGAWRIHRTLNPARDKKDERPTLASTTLSRAIGGARATAALPPRSAESSITEGTT
                     ELLEAPAREFVPIELRHRDGDTGPIN"
     gene            complement(7742..8959)
                     /locus_tag="VF240_10810"
     CDS             complement(7742..8959)
                     /locus_tag="VF240_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7175739.1"
                     /translation="MYVVMIAPGYPAEMPYFTRGLARMGARVLGVGDQAEHDLPAVTR
                     EHLSAYLRVPCLTDEEAVVEAVRRWAAPVRVERVECLWEPGVILAARVREALGVEGMT
                     AEEAVPFRNKDRMKQVLSAAGVRTPRHALAHTADACRAAADSIGYPLILKPIAGAGSA
                     DTHRADDAAQLDAAIAQLGHLSEVNVEEFIDGEEYTFDTVCVDGEIKFYSISWYRPRP
                     LIARTVEWISPQIVMLRDVEAEHLARGREMGRAVLDALKFRTGFTHMEWFRKADGQVV
                     FGEIAARPPGARSVDVMNYACDLDLFNGWAEATVRGRFTQAVERRYNAVIIFKRAQGC
                     GRIRRVEGLERILSHYGEHVACIDLLGPGEQRRNWRQTLISDGYLVIRHPDLSRALEI
                     ADAVGTDLQLYAG"
     gene            complement(9062..10282)
                     /locus_tag="VF240_10815"
     CDS             complement(9062..10282)
                     /locus_tag="VF240_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7175740.1"
                     /translation="MPLVIFVAPWFSEFAAHSIDALAGLDGVRVALISQEPLESLQSP
                     LRDRVAAHWRISDAFDAAQITDAAHALSRRLGPVHRLIGVLEQAQVAIAEARERLGVA
                     GMGAEAARNFRDKARMKTLLREAGVPCARHRLAASEAEAFAFAAEVGYPLVVKPPAGA
                     GSVSTFRVDDDAALGDALRVSAPDAHNPALLEEFITGEEHSFETMSLDGHAVWHSLTR
                     YYPTPLEVMRNPWIQWCLLLPREIDDARYDDIRRAAARALQVLGMDTGLSHMEWFRRA
                     DGTVAISEVGARPPGAQITTMNSRAHDIDFMAAWARLMVYGEFAPPERRYAVGAAFLR
                     GQGRGEVRAVHGLEEAQQEFGHLFTDVKLPREGQTPSSSYEGEGYVILRHPDTFVVEQ
                     ALSRLVSLIRVELG"
     gene            complement(10428..12305)
                     /locus_tag="VF240_10820"
     CDS             complement(10428..12305)
                     /locus_tag="VF240_10820"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEX7175741.1"
                     /translation="MTRLPSRALSLCVLAAALCASAHAQTPTGAGEQAARTATPATTT
                     ADRAAGGGDAQPLDEVLRLLREQRQEIERLRAQLEEHARAVGELRARVERAEQAAPAP
                     TRGATFAGALYTKDGDAADSAGETVAPRGAQGAQAEDRLGRVEEQVKKTSEALTKQLG
                     SITFSGDLRMRWEPTFGQLNALPNSDDPAVLGNELSARHRFRMRARLAVRGNFGKEVF
                     LGTYDREGKKQLGREFEWGMRLATGNFPDIISSNQTFTDFFSRKSFALDQAYVTYRPP
                     SLTGLQLQAGKFDVPWLRTEMTIDNDLTVEGFNESYTRSFKKSALKTLTFVAWQLPFL
                     ERNSAFVVGADGRVNLEQSERGGRDLALYGAQLRARFEPTKNTALTLAAADLFYSGTQ
                     FITPAQVFGGNLQVPVTVTIPATATAPAQTVTGFATIARDQLVSGNANLGASIASNNA
                     TNRDGRLSSGFNLVDSIVRFDYTRSKRWPVMLLVNFVRNTQAHDLVLAGGPGGTNRIV
                     PNDEDTGLWAEFQVGKDVTRLAPKDVAHGDMFFNYTFTRIEKDAVLTPFNFSDFAQQS
                     DFRGHRFIIAYAADPRVTLSLTGLVTERPHGLLGVFGATPPGSLNRPTTRLQLDTVFR
                     F"
     gene            12667..14394
                     /locus_tag="VF240_10825"
     CDS             12667..14394
                     /locus_tag="VF240_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7175742.1"
                     /translation="MALLHIVDAGGRQWELSLTGQSVCTIGRAPDNSVVLTDARASRY
                     HAHITQRDGDYLIIDGKPGGGRSANHVYVNGRQIEEHALTDGDRILVGASQLRFARSA
                     EDAPRTGVSYDDRPLGHTQLLVSATDVINSALQSRPRGSSALLPTEPAVLAADGELEQ
                     LRRKAKILALLYEMSKTLGSVFNLDAIFEKATQVIFDATPADRVVALLSDDSAASAAA
                     AAAVAHGEGPELHVASLRVRDPAREEQARRQTIGRTITRKVMRDRAALLSQDAAADAE
                     FMGVHSIVMQGVRSTICAPMIAESGVHGALYADRLDQFTSFTRDDLELISAVAAQAAV
                     AVENARAHVRLAREEVARANYSRFLPGYVVQQILEDPDSFKLGGVNQTVTVLFADIRG
                     FTSLSEAAPPEKVVQLLNRYFTAMSDIIFAHGGTLDKYIGDGLMALFGAPTETPEDAC
                     NAVAAAVAMQRQMSVINTELRREGFEGIQIGIGLHTGEATVGYIGSERRSEYTAIGDT
                     VNLASRLESGALPGQILLTEASAAAAREAGCLFRPREAIVVKNRQRPVPVFDVDWSEA
                     AEGATPSRV"
     gene            14475..14936
                     /locus_tag="VF240_10830"
     CDS             14475..14936
                     /locus_tag="VF240_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7175743.1"
                     /translation="MADYNSLASRCAPYLLSVLRIVSGLLMLQHGTQKVFGWPPPAAP
                     GARPPAAFDPTSLMGIVGLLELFGGLLLLIGLFTRPAAFILSGMMAVAYFIGHFSIGA
                     FWPIQNSGELAVLYCFIFLYLSAAGGGPWSVDACLPGRRGGADASDAGRGY"
     gene            14989..16092
                     /locus_tag="VF240_10835"
     CDS             14989..16092
                     /locus_tag="VF240_10835"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7175744.1"
                     /translation="MTFMGKTRTLLSLTGLRELVSDSMSIDLGSAHTVISVRGRGVVV
                     DEPSIVAVHRATGEVVAVGREAHEMQGREAREVTLVRPLKNGVVADFERTQKMLEHFV
                     REARSGISQFSRRAVMSVLSGVTHVEQRALLSAAEQARIGRVYMVEEGLAAAIGAGVS
                     IQDRAASVVVDVGGGSTNIAVVARGSIIYSQAERIGSDDIDDAIIDRLRRHHGLLVGA
                     QTAERLKRELGSAVEPANPALSETVKGRDAQQGTPRAIEVTSEEVCIAAEPVVSRLSR
                     VVQRALAELQPEVAADVYDRGLILTGGGALLDGLAELLQRDTRLRTRVAEDPRHACVR
                     GLQQLFDDPLLLRRVSRNEPHLLLDSDAGAYDV"
     gene            16173..>16957
                     /locus_tag="VF240_10840"
     CDS             16173..>16957
                     /locus_tag="VF240_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7175745.1"
                     /translation="MSFFKRQIQVARVAGIPVRIDYRWFLVFALSAWVLAASFQQPAS
                     VLTQGVHLDAGAAWLAGVATTIGLFISIFGHELSHALAGRMEGIESEEIVLHPFGGFT
                     RLRREPDSPRAEFRIAIAGPSSSFLLALVFFLLFAAARFANYRAVAAGLSIVAYWNLF
                     IAVSNLLPGYPLDGGRVLRAFLWKRYGRLDEATRIASLGGQLIAGTLVVFGVYFYWKL
                     NDAFRGLWLVLIGLFLWDAARSVYGGRRRGARTVADAMGAPVAV"
BASE COUNT         2939 a         5617 c         5412 g         2989 t
ORIGIN      
        1 acagcgcatg cagatcatcg ccgtgcgcca gcgcgacacg cgcatggtcg gctgcacggg
       61 cgacgcgcag atcatcatca gcaactccga cctcgaagac ttgatcgtct ccgtcctcga
      121 agcctgcgac tcgccggccg acgtgcgcac gcttcgtagc ctcgtcatgt cgcgcctgcc
      181 cgtgatggac atctatctcg tcccgctggg cggcgacgac gcggaggacg gccaccgcgg
      241 cttcgacccc gtggacgggc gcgagaaccc cgagcaagtg ctgctcagac gggaggcgga
      301 gagtgggcga gacggtttgg tggatcggtt catggaaggc ctgtcggcga gtgtgcgcgg
      361 caagaccaag cagtacgacc ggatgctcgg cgtgctgtgg cactgctacc tgaacccggt
      421 gcacgtgacg cagctcgaag tcgccgcgat gctcggcgtc tccgattcgc tcgtcacaga
      481 ataccggcgg cgcatcgagc gcgagttacg cgcactcgct atagcgggtg tcgccgaggc
      541 gcgcgccttc gaggcggcgc tgagggaacg tgtttcgcgc cttccgggct gctcgcctcc
      601 cgcgaggtcg agccgtctgc agacggtcgc gtgaacgtgc tactgccgct ttattagcgt
      661 tcgggttctt gtgctcgcgc ggtttcgagt agagggcggt ggctgatagt gggcgctcga
      721 cttacctttt tggaattgaa caaaataacc attctgttca attatgccat atccgagatg
      781 atgttttatt gaggcttctg tggataaaag agtatgggat ttttactgta atgttcaatt
      841 tggaaattat atggtggtcc cacagatcgc cacctataga gggctttaag ccacgttaac
      901 ctgccgcccg ccctccgccg ccacgttggt ctgcacctcg gggacgcggg ccgagagctt
      961 ccgcacacgc tccatcgact cgcacgcccg cgtgaacctc ttctgcgccg ccgtcagccg
     1021 cttctcccag tacgcgctca gcgtcagcgt gattgattgc cgcatcacgc ccgtgtagcg
     1081 cagctcgatc atcgacaggg gcgggagtga agaggtgtgg caccgcctga ctattgacaa
     1141 ccgcgcgccc cgtcaatcct cctcccgctc ctcttcaatt ctctgctcca tcgcttctcc
     1201 ttcctattac ggtttgcgct tgcattggca ctatgagtgc ccacactcat aagccatgca
     1261 agcctattcg actgatttac gagagaaggt tgctctcgct tatgaacatc acgattacac
     1321 tttggacgaa gtggccgaac tcttcgatgt cggacggcgc accgtcgccc gcttcgtaca
     1381 caagcaccgc gcggggttga gcctcgcgcc gcagccgcac gccgggggct acccgaccac
     1441 gctggctgcc aagacactct cgacgctgcg cgacaaagta gtcgagacgc cggacgcgac
     1501 gctccgcgaa ctcgccagct atttgaaaac caaagcccat gtgcaagttc atccctcgac
     1561 cgtctgccgc gcgctgcaac gcttaggtct gccgcgaaaa aaaagacgct cgccgccaac
     1621 gaaagggatg aagcacaacg actcgccttc cggcagcgga tggagagcat tgatcgccgc
     1681 aagctcgtct ttactgatga aacgggcttt cacctggctt tcacgcgcgc cttcgggcgt
     1741 gccccgcgtg gccagcgtgt ggtcggccat gtgccgttta agcgcgggcc gaaccattct
     1801 ctgatcggca gcctcgggct gcgcggcgtg gtcgcctctt tgttgctcac gggcagcgtt
     1861 gaccacttgg tcttcgacgc ctttgtcaat gaactgttgt tgccacggct gcgcgcgggg
     1921 gatgtgctgt tgctcgacaa tttacctgcg cataaagcca gcgccgtcga agagtcagcc
     1981 gccagagcga gagcgcaggt cagctggctg ccggcttact cgccagactt ctcgccaaac
     2041 gagaactgct ggctcaagat caagaccttc gtgcgtggtc agcagccacg caccgcgccg
     2101 gacttgaacg aggctctcag tgcagccatc cgagccgtgt gcaaacaaga cattgcggct
     2161 tggttcaaac attgtggcta ttagtgccta tgcaactgca aaccgtaata agtgttaaga
     2221 aaggcggggc gggaacgggc agtgttgacg atttcgttgc cgtcatcacc gaagacggag
     2281 gcacttgcgc cgtgcggctc cacgacgggc cgggcggcta cccgagttac aaatgctcat
     2341 ccgggctgcg ccccggtcat ccggaaaaag atttcgggac gagttctaac gacttttggg
     2401 ccggcatgtg ataaaactgc cgcgccgaca cggggacagg caccggatac tttactcacg
     2461 agttacgcac cctatctcca atcacccacg acctacagca aggagattta gaaatgacga
     2521 gactacgctt ccccttccga gtcttagggc tcgccatcct caccctcgca ttcaccacgg
     2581 cggcacaggc acaggcgacg aggacgttcg tgtcaggcgt tggcaacgac gctgacccgt
     2641 gcagccgcac cgccccgtgc cgcaccttct ccggcgcgct catcaagacc ttcatcaacg
     2701 gcgagatcaa cgcgctcgat ccgggcggct acggcacgct gaacgttacc aagtccatca
     2761 ccgttgacgg caccaacggc tccggctacg gctcgattct cgccagcggc accaccggcg
     2821 ttatcgtcaa catcgccccc ggtaacgtca acgacccgca ccgcagcgtg cgcctgcgca
     2881 acctctccat caacggcacg gggccgagcg gcgccgtcgg cacacgcacc ggcactgacg
     2941 gcgtccgcat gatcgccgga tcctcgctca tcatcgacaa cacggtcatc aacgacttct
     3001 cacaggaggg cgtcgaggtg gccggcgggg tcgagggcaa ccagatcacc gtcgtcatca
     3061 acaacacggt gattcgcaac tgcaacggca ccggcatcag gctggcgacc ggcgcgggct
     3121 cgattggcgc catgctcagc gacgtgcgcg tccacacctg cgccaccggc ctattgggcg
     3181 ccaaccgcac ccgcatcaac gtcaagaacg gggtcttctc gcagaacact acggggctga
     3241 atcagaccgg cacggacaac atcacgaacg tggaggacgt cttcgtcacg atcgccacga
     3301 ccggcatcca gtcgagcgcc ggcaacatca cccgcgtctc gaacagcgtc gtcacccaaa
     3361 ccctaacggg gctgaacccg aacgggggca ctttacactc catgagcggc aacgccgtct
     3421 tcggcaatac cgctgacggg gctttcaaca gcgggcctac tcccaagtcg taggctgaac
     3481 gcgactcgtt ccacacccgg cgcccccgtc cgctccccga gcaggcgggg gcgccggtca
     3541 tgtctcggcc ccttaaatcg ctctgtcctg ctccgcgtag tgttcgcggt aagctccgcg
     3601 cgcgcgcgac ccgtgcgcgg ttctcggtgt catcttgaga gccgcgctgt tcgcgcgtcg
     3661 gatgcctgcc gttgccagac ctcgaattgc cttacggcta cgactcctgg tttttcttcc
     3721 ctgccgatat agttcgtcaa ggtcatttgt tttctttgaa gatttggccg ttcttcatga
     3781 caaaggtcac cgccttgagc gtgtcaaggt tgtcgagggg attggctgcg gtcgcgatga
     3841 tgtccgcttt catcccgacg cgcagcgcgc cgcgttcctt ctcgacgccg agcaggcgtg
     3901 cggcgttcgt cgtcaaagct tgtaaaatca cccgtgccgg gatgcccgct tcaacccagt
     3961 tgtctatcgt ctcgatggac aacctgccgc gcgaatactc cttcgtcgtg aagattacgt
     4021 cggggccgaa aacgagcgta acgccgatct gatgtgcgcg cttgaccggg tcgataaacc
     4081 accgctcgtt gacctgcttg ttggctcccg ccgtgcggaa ctcccgagca tccgtctccg
     4141 taaagggggt cgggacgagc gccactccat tgcgcttcgc gatcaccaac gtctcgttcg
     4201 acatggcgac gccgtgctcg atggaggcga cgccggcctc cgccgcgttg cgcgcgcccg
     4261 cttccgtcca gcaatgggcg gcgaccttta gcccggcacg tgcagcttct tcaatcacaa
     4321 agcggatgtc ttcgacggag tagatgtatc gctgatcgtc aacgaccagc ttgatgacgc
     4381 gcgctccgta atggatattc tctcggatcg ccttttttag ttcatcgcgt gtgtcggcgt
     4441 aaaaatactc cgggttgccg agaccgggtt tatccggttg catctgaaat tgtccgccaa
     4501 acggcgcgat gatccggccg gcgttgataa ttgtcggccc cggcaccagc ccttcatcta
     4561 tcgagcggcg caggtcagtg tcggcataat tacccgcgtt gccgatgtcg cgcacggtgg
     4621 tgaatccggt ctccagcatc gaacgagcat tagcaacgcc ctgtatcgcg cgataaccgt
     4681 tcgagtcgag aagagttgta aagtagtagt tgccgccatc gcgcccccgc cgcgtcgtca
     4741 tacacagatg cgtatgtgcg tcgaacaagc cgggcagcac cgtccggttt gataaatcga
     4801 tgaccaacgc cccggcgggg attttaacgt cagggccaac ggccttgatc gtttggcctt
     4861 cgacgagaat gatctgattg ccggtgacag tacccgcctc cgggtcaacc agcttaccag
     4921 ccttaatcgc cgtcacttgc gcacgtccaa cggcgtggca gaagacgatc aacactatga
     4981 cgaatactaa cttgaaagcg ttcgtaaatt tcatacatcc tccgattcac aaagccgccc
     5041 gacgcccgcc atcagccgcc gcgcacaccg attaccaaac cgctccacaa ggcgaaggcg
     5101 agagctgagg tcgtgaggcg catgggtttg cggattaaat caaaccgcgg cgcaaaagga
     5161 aaggcgcccc tgataaccca cccgatccgc tcctccatcg cctcggtttt taatagcgag
     5221 agggagccgt cacaaccacg cccctcgcta ttgacatccg cccggcccca gcttatcttt
     5281 ccgcccgtcc agcagggcgc ttacctcact accggcgcgc aaacacttct tgatgaggag
     5341 aacattaatg cccgataacc agcagaacac tgatccgacg acgccctccg agcctgagac
     5401 ggaggcacaa gccccggccc ctgtgagcgc gacgacacag gccgcgctcg actctcccaa
     5461 caccgtcacg gtcaatcccg gcgcgcagac cttcccgacc atcggggcgg cgctcgccag
     5521 cattaccgac aaccgcttgc agaagcaata cgagctgatc gtcggccccg gcacttataa
     5581 cgagaaggtg acgatgaagc cctttgtcac catccagggc gcggggcagg acgtgaccat
     5641 catcacgcag cccgccgcat cggacgcctt cagcggcggc accgtcatcg ccgccagcaa
     5701 ttgcacgctc gccaatgtca ccgtcagcag cctcggcggc cactggggtt cctacagcac
     5761 ggcgctctct tgcagcggcg tcaccggctt ctcgtgcgac gcttcaacct tgctcgccga
     5821 cgaccagaat caggcgggca ttaatatgtc cactctcaac gcgtcgttga ataccatcga
     5881 acccgcctcg caaatcattc tttcttactg caccatcatc tgcaacgcgc agagcaacca
     5941 gtctggcgcc gtcagcgtcg gcatcgccca gaactcaacc gccgaactac tggacacaaa
     6001 aatcgtcgcc accggcggct cgcagtcctt cggagtcatt accgcccgcg gcagctccgt
     6061 caccctcgac gactgctaca ttcagggcag ctctttcgcc ctttacgact ccgactccag
     6121 ttctcccatc accgccaaca actgccagat taacggcaac atctccaacg gcgtgacggt
     6181 caacaacaat ccgccgccga caagcgagta ggaaggatga atgcggaaac gatgaacgat
     6241 gaaggaaaag caggctctta acagggatgt attcgttatc cggaaagcga tctaacgccg
     6301 ggcatcaccc gcgcccctca gcaacgaaca tgagaaacac gctgaagtgg cgtctgctgc
     6361 tcgatggtct gaccgggtga ttaatttcgg aaggggccgc ggcggccgtt atccgcacgg
     6421 ctgccgcggc cccttccaag cgcagagccc gtgagggttc gtcaaggggg gtgttgcgac
     6481 gatgctatgc gaaggaggct aggaaaagat taacggaggc ttaaatgttc gttacactaa
     6541 tagcggtcag cattcagcta tcagcagtta gccaaacacg agccgaagac gcgttgccgc
     6601 cgatgaataa atctctggtt cgccatcttc cggctgaacg ctgatagctc atcgctgatc
     6661 gctcctccat tgaaacacgg cccggccggc gctcgtattc tgtgactgag aacgacgaaa
     6721 ggagccggac gcggatgggc gcagagattt ggatggcctc gatgcaggcc gagggtgacc
     6781 acagcgtgat gcgcgtgatg gcgggcgacg ccgacgacct gcgcgcgcgt ctcgccgacg
     6841 ccgtcgagag gctcggctac cacgtcatct ccgatcaggc ggcgctcgtc gcgcggcgcg
     6901 gcaaccgccg gctgggccgt tcggggtgct ccttcgatct gcgcgactac gccaccgagc
     6961 tgatcgtcac gctcaagccc aagagcgcgg gcgtcacgct cgccaccttc aacttcaccg
     7021 tcaggaatta ttactactgg acgggcggcg acaagcgcac gctcgaatgt gaggccgacg
     7081 ccctcgccgc gctcgccgtc tcgcagcagt ggctcacggc ctgcaccgcc tgcggcaccg
     7141 agacgaccga cgactcgcgc ttctgccgcc gctgcggcgc gccgctcgcc ctcgaagagc
     7201 cggccgagct tgaggcgctg cgtctcaccg ccggcacgcg cgccggctat aagaacatcg
     7261 cgtggggcct cctcgtgctg gtgtcgatac tcatccccgt cgtcatcctg ctcacgctcg
     7321 gcgcgtcgga gaaggcattc aacgccgcgg cgatcatcgc ggcctcgggc ggcggctccg
     7381 ccctcttcat gctgctcgcc ggcgcgtggc gcatccaccg cacgctcaac cccgcgcgcg
     7441 ataaaaaaga tgagcggccc acgctcgcgt ccacgacatt gtcgcgcgcc atcggcggag
     7501 cgcgcgccac ggccgccctg ccgccgcgct ccgctgagtc ctccatcacc gagggcacga
     7561 cggaactcct cgaagccccc gctcgcgagt ttgtccccat cgagctgcgc caccgcgacg
     7621 gcgacacggg gccgatcaac tgaaaaacag tgccgcgagc ggtaacgagc gggtgcgcct
     7681 gcgacgagag ggccgcacca ctcgtccaga cccgctcgct accgctcgcg gtactgtgtt
     7741 gctagccggc gtagagttgc aggtcggtgc cgacggcgtc ggcgatctcc agcgcgcggc
     7801 tgaggtctgg gtgacggatg acgaggtagc cgtcggagat gagcgtctgc cgccagttgc
     7861 ggcgctgctc gccgggcccg agcaggtcga tgcaggcgac gtgctcgccg tagtgtgaga
     7921 ggatgcgctc cagtccttcg acgcggcgga tgcgcccgca cccttgcgcg cgcttgaaga
     7981 tgatgacggc gttgtagcgg cgctccacgg cctgcgtgaa gcgcccgcgc accgtcgcct
     8041 cggcccaacc gttgaagagg tcgaggtcgc aggcgtagtt catcacgtca acggagcgcg
     8101 cgccgggggg acgcgcggcg atctcgccga agactacttg gccgtccgcc ttgcggaacc
     8161 actccatgtg tgtgaagccg gtgcggaact tcagcgcgtc gagcacggcg cgccccatct
     8221 cacgcccccg agcgaggtgc tcggcctcca cgtcgcgcag catgacgatc tgcgggctga
     8281 tccattcgac ggtgcgcgcg atgagcggcc gcgggcgata ccacgagatg ctgtaaaact
     8341 tgatctcgcc atcgacgcag accgtgtcga acgtgtactc ctcgccgtcg ataaactcct
     8401 cgacgttgac ctcggacaga tgcccgagtt gcgctatcgc cgcatcgagt tgcgcggcgt
     8461 cgtcggcgcg gtgcgtgtcg gccgagccgg ccccggcaat cggtttgagg atgagcgggt
     8521 agcctatcga gtcggcggcc gcgcgacagg cgtcggccgt gtgggcgagc gcgtggcgcg
     8581 gggtgcggac gccggccgcc gagagcacct gcttcatccg atccttgttg cggaagggga
     8641 cggcctcctc cgccgtcatg ccctcaacgc ccagcgcctc gcgcacgcgc gcggcgagaa
     8701 tcacgcccgg ctcccacaaa cactccacgc gctcgacgcg caccggcgcc gcccagcgcc
     8761 gcaccgcctc gacgaccgcc tcttcgtccg tcagacacgg cacgcgcagg taagccgaca
     8821 ggtgctcgcg cgtgaccgcc ggcaaatcat gctcggcctg atcgcccacg cccagcacgc
     8881 gcgcccccat ccgcgccaac ccacgcgtga agtagggcat ctccgccgga taaccgggcg
     8941 cgatcatcac cacgtacata agtgctcctt aagagagagt tcaaagttca aagttcaaag
     9001 ttcaaagtca acagccgccg gacgcgcttt gaactttgaa ctttgaactt tgaactccgg
     9061 gtcagccgag ttccaccctg atgagcgaga cgaggcggga cagggcttgt tcaaccacga
     9121 acgtgtcggg gtggcggagg atgacgtagc cctcgccctc gtagctgctc gaaggcgttt
     9181 gtccttcgcg gggcagtttc acgtccgtga agaggtggcc gaactcttgc tgcgcctctt
     9241 cgagaccgtg gacggcgcgc acctcgccgc ggccctgccc gcgcaggaaa gcggcgccga
     9301 cggcatagcg gcgctcgggc ggggcgaact cgccgtagac catcagacgc gcccacgccg
     9361 ccatgaagtc gatgtcgtgc gcgcgcgagt tcatcgtggt gatctgcgcg ccggggggcc
     9421 gcgcgcccac ctcggagatg gcgaccgtgc cgtccgcgcg tcggaaccat tccatgtggc
     9481 tcagccccgt gtccatgccg aggacttgaa gcgcgcgcgc ggcggcgcgg cggatgtcgt
     9541 cgtagcgcgc gtcgtcgatc tcacgcggga gcagcaggca ccactgtatc caagggttac
     9601 gcatcacttc gagcggcgtc ggatagtagc gcgtgagcga atgccagacg gcgtggccgt
     9661 cgagcgacat ggtctcgaag gagtgctcct cgcccgtgat gaactcttcc aagagggctg
     9721 ggttgtgcgc gtcgggcgcg ctcacgcgca gtgcgtcgcc gagcgccgcg tcgtcatcga
     9781 cgcggaaggt ggacaccgac cccgcgccgg ccggcggctt cacgacaagt ggatagccaa
     9841 cctctgcggc gaaggcaaac gcttcggcct cgctcgcggc gagccggtgg cgcgcgcagg
     9901 ggacgccggc ctcacgcaga agcgtcttca tacgcgcctt gtcgcggaag ttgcgcgccg
     9961 cctccgcgcc catcccggcg acgccgaggc gctcgcgggc ctcggcgatg gccacctgcg
    10021 cctgctccag cacaccgatg aggcggtgga cggggccgag gcgacgggag agcgcgtggg
    10081 cggcgtcggt gatctgcgcg gcgtcgaagg cgtcactgat gcgccagtgg gcggcgacgc
    10141 ggtcgcgcaa cggcgactgt agactttcca gcggctcctg actgatgagc gcgacgcgca
    10201 cgccgtcgag gcccgcgagc gcgtcaatgg agtgcgcggc gaactcggag aaccacgggg
    10261 cgacgaagat gacgagcggc atgacgggcc tccgtgtctg acggctacga ggctggcaga
    10321 atttggcggc gagtatacgg aagagggggt aaggggtaaa ggggaagggg taagggagaa
    10381 gacatattct ttcccttacc ccttcccctt taccccttac cccgttctca gaagcggaag
    10441 accgtgtcga gttgcaggcg cgtggtcggg cggttgagcg agccgggcgg cgtcgcgccg
    10501 aagacgccga gcaggccgtg ggggcgctcg gtgacgaggc ccgtgagcga gagcgtgacg
    10561 cgcgggtcgg cggcgtaggc gatgatgaag cggtggccgc ggaagtctga ctgctgcgcg
    10621 aagtcgctga agttgaacgg cgtgagcacg gcgtccttct cgatgcgcgt gaacgtgtag
    10681 ttgaagaaca tgtcgccgtg cgcgacatcc ttcggcgcaa ggcgcgtcac atccttgccc
    10741 acctgaaact cggcccagag gccggtgtct tcgtcgttgg gcacgatgcg gttcgtgccg
    10801 cccggccccc cggcgagcac gaggtcgtgc gcctgcgtgt tcctgacgaa gttgacgagc
    10861 agcatgacgg gccaacgctt gctgcgcgtg tagtcgaaac gcacgattga atcgacgagg
    10921 ttgaagccgg aggagaggcg tccgtcgcgg ttggtggcgt tgttcgaggc gatggaggcg
    10981 ccgaggttgg cgttgcccga gacgagctgg tcgcgcgcga tggtggcgaa gccggtgacg
    11041 gtctgcgccg gggcggtggc cgtcgccggg atggtgacgg tgacgggcac ctggaggttt
    11101 ccgccgaaga cctgtgcggg cgtgatgaac tgggtgccgg agtagaagag gtcggcggcc
    11161 gcaagcgtca gcgccgtgtt cttcgtcggc tcgaagcgcg cgcgcaactg tgcgccgtag
    11221 agcgcgaggt cgcggccccc gcgctccgac tgttcgaggt tgacgcggcc gtccgcgccg
    11281 acgacgaagg ccgagttgcg ctcaaggaag gggagttgcc acgcgacgaa ggtgagcgtc
    11341 ttgagggccg actttttgaa gctgcgcgtg tagctttcgt tgaagccttc gacggtgagg
    11401 tcgttgtcga tggtcatctc ggtgcgtagc cacggcacgt cgaacttccc ggcctgcaac
    11461 tggaggccgg tcagggacgg cggcctgtag gtgacgtaag cctgatcgag cgcgaagctc
    11521 ttgcgcgaga agaagtcggt gaaggtctgg ttcgacgaga tgatgtcggg gaagttgccg
    11581 gtggcgagcc gcatgcccca ctcgaactcc ctgcccaact gcttcttccc ctcgcggtcg
    11641 taagtgccga ggaagacctc tttgccaaag ttcccgcgca cggccagacg cgcgcgcatg
    11701 cggaagcggt ggcgcgccga gagttcgttg ccgagcacgg cggggtcgtc gctgttgggc
    11761 agcgcgttca actgcccgaa ggtcggttcc cagcgcatgc gcaggtcgcc gctgaacgtg
    11821 atcgagccga gctgtttggt gagcgcctcg ctcgtcttct tcacctgctc ctcgacgcgc
    11881 cccagacgat cctccgcctg tgcgccctgc gctccgcgcg gcgcgacggt ttcgcccgcg
    11941 gagtcggccg catcgccatc cttcgtgtag agagcgccgg cgaacgtcgc gccgcgcgtg
    12001 ggggcggggg ccgcctgctc ggcgcgctcc acgcgcgcgc gcaactcgcc gacggcgcgc
    12061 gcatgttcct ccaactgcgc gcgcaggcgc tcgatctcct gtcgctgctc gcgcagcagg
    12121 cggagcactt cgtcgagcgg ctgcgcgtcg ccgccgccgg cggcccggtc ggcggtagta
    12181 gtggccggcg tcgcggtgcg tgctgcctgc tcgccggcgc ccgtgggagt ttgcgcgtgg
    12241 gccgaagcgc agagcgcggc ggcgaggacg caaagagaca gggcgcgtga cgggagtcgg
    12301 gtcatgtgtc gatcctttct gttgacggtt gtgccccggc ggacgaacga ctggtaagag
    12361 acgacatcat aatagacgcg gccttaacgg caaattacgt ttatgtgaac aaagggttac
    12421 aagtggcgag agggttcagt agtccgttgt tcatagccgg tggtgatgga gctatcagct
    12481 tttagctttt agcggttagc cagaagagag cgaaccggag atttgttaag cggcggcgac
    12541 gcgcctcggg ctcacgtttc tggctgaaag ctaatagcta atagctgaca gcttcattgc
    12601 cactgacaac tgacgacttt gcatgtagaa tttcggcgtt cccatctcgc gcacagagaa
    12661 agccgtatgg cccttctgca catcgtcgat gctggcggac gccagtggga gttgtccctc
    12721 acggggcagt cagtgtgcac catcgggcgc gcgcccgaca actcggtcgt gctcacggac
    12781 gcgcgcgcct cgcgctacca cgcgcacatc acgcaacgcg acggcgacta tctcatcatc
    12841 gacggtaagc ccggcggcgg ccgcagcgcc aaccacgtct acgtcaacgg ccggcagatc
    12901 gaggagcacg cgctcaccga cggcgaccgc atcctcgtcg gcgcgagcca gctccgcttt
    12961 gcccgctcgg ccgaggacgc gccgcgcacc ggcgtctcct acgacgaccg ccccctcggg
    13021 cacacacaac tgctcgtctc cgcaaccgat gtaattaact ccgcgctgca atcgcggcca
    13081 cgcggctcgt cggcgctcct gcccaccgag ccggccgtgc ttgccgccga cggtgagctt
    13141 gagcagttgc gccgcaaggc gaaaatcctc gccctgctct acgagatgtc gaagacgctc
    13201 ggctcggtct tcaatctcga cgccatcttc gagaaggcca cgcaggtgat cttcgacgcc
    13261 acgccggccg accgcgtcgt cgcgctgcta tcggacgaca gcgccgcgag tgccgccgcc
    13321 gccgccgctg tcgcgcatgg cgaggggccg gagttgcacg tggcgtcgct gcgcgtgcgc
    13381 gacccggcgc gcgaggagca ggcccgccgg cagaccatcg ggcgcaccat cacgcgcaag
    13441 gtgatgcgcg accgcgcggc gctgttgtcg caggacgccg cggccgacgc cgagtttatg
    13501 ggcgtccact ccatcgtcat gcagggcgtg cgctcgacga tctgcgcgcc gatgatcgcc
    13561 gagtcgggcg tccacggcgc gctctacgcc gaccgcctcg accagttcac ctcgtttacg
    13621 cgcgacgacc ttgagctgat ctcggccgtg gcggcgcagg cggccgtggc ggtggagaac
    13681 gcgcgcgcgc acgtccggct cgcgcgcgag gaggtggccc gcgccaacta cagccgcttc
    13741 ctccccggct acgtcgtcca gcaaatactc gaagaccccg actccttcaa gctcggcggc
    13801 gtcaaccaga ccgtcaccgt gctcttcgcc gacatccgcg gcttcaccag tctctccgag
    13861 gccgcgccgc cggagaaggt cgtccagctt ttgaatcggt atttcacggc gatgtcggac
    13921 atcatcttcg cgcacggcgg aacgctcgac aagtacatcg gcgacgggct gatggcgctg
    13981 ttcggcgcgc cgaccgagac gcccgaggac gcctgcaacg ccgtcgcggc ggccgtcgcc
    14041 atgcagcggc agatgtcggt catcaacacg gagctgcgac gcgagggctt cgaggggatt
    14101 caaatcggca tcggtctgca cacgggcgag gccaccgtgg gctacatcgg ctcggaacgc
    14161 cgctcggagt acacggccat cggcgacacg gtcaacctcg cgtcgcgtct ggagtcgggc
    14221 gcgctgcccg gccagatact tttgacggaa gcctcggccg ccgcggcgcg cgaggcgggc
    14281 tgtctcttcc gaccgcgcga ggccatcgtc gtcaagaacc gccagcggcc cgtccccgtc
    14341 ttcgacgtgg actggagcga ggcggccgaa ggcgcgaccc cttcgcgcgt ctgagcgggg
    14401 aagacggcgt gcgagttcta acgattcaag cgctctaaca attcagaagc accgactgac
    14461 ttgagggaga tggcatggct gattacaact ctttagcttc cagatgtgcg ccgtacttgc
    14521 tgagcgtcct gcgtatcgtc tccggcctgc tcatgttgca gcacggcacg cagaaggttt
    14581 tcgggtggcc gccgccggcc gcgcccggcg cacgcccacc cgcggcgttc gatccaacgt
    14641 cgctgatggg tatcgttggg ctgctggaat tattcggcgg gctgctgctg ctcatcggcc
    14701 tcttcacgcg gcccgcggcc ttcatcctgt cgggcatgat ggcggtggcc tacttcatag
    14761 gccacttctc aataggggcc ttctggccga ttcagaattc cggcgagttg gccgtgctct
    14821 actgcttcat cttcctctac ctgtcggcgg cgggcggcgg cccgtggagc gtggacgcct
    14881 gcctgccggg gcggcgcggc ggcgctgacg cttcagacgc agggcgcggc tactgacgat
    14941 cttttgcggc cggacgagca cgagcgcgcc cggccgcgag ttcaacttat gacgttcatg
    15001 ggaaagacgc gcacgctgct gagcctcacg ggcctgcgcg agctggtttc ggattcgatg
    15061 tcgattgacc tcggctcggc gcacacggtg atctccgtgc gcgggcgcgg cgtcgtcgtc
    15121 gatgagccgt caatcgtcgc cgtccaccgg gcgacgggcg aggtggtggc cgtcgggcgc
    15181 gaggcgcacg agatgcaggg gcgcgaggcg cgcgaagtga cactggtgcg gccgctgaag
    15241 aacggcgtgg tggccgactt cgagcgcacg cagaagatgc tcgaacactt cgtgcgcgag
    15301 gcccgcagcg gcatctccca attcagccgc cgcgccgtga tgagcgtgct ctcgggcgtg
    15361 acgcacgtcg agcagcgggc gctgctgtcg gccgccgagc aggcgcgcat cggccgcgtc
    15421 tacatggtcg aggagggatt ggcggcggcc atcggcgcgg gcgtctccat acaggatcgc
    15481 gcggcctcgg tcgtcgtgga cgtgggcggc ggcagcacca acatcgccgt cgtcgcgcgc
    15541 ggctcgatca tctactcgca ggccgagcgc atcggcagtg acgacatcga cgacgccatc
    15601 atcgaccgcc tgcgccgcca ccacggactg ctcgtcggcg cgcagacggc cgagcggctc
    15661 aagcgcgagc tggggtcggc cgtcgagccg gctaaccccg cgctctccga gaccgtcaag
    15721 gggcgcgacg cgcagcaggg cacgccgcgg gccatcgagg tgacgagcga ggaagtctgc
    15781 atcgccgcgg agccagtggt cagccgcctg tcgcgcgtcg tgcagcgcgc gctcgcggaa
    15841 ctccaacccg aggtggccgc cgacgtttac gaccgcggcc tgatcctcac gggcggcggc
    15901 gccctgctcg acggccttgc cgaactcctc cagcgcgaca cgcgcctccg cacacgcgtc
    15961 gccgaagacc cccgccacgc ctgcgtccgt ggcctccaac aactcttcga cgacccgctc
    16021 ctcctccgcc gcgtctcccg caacgagccg cacctcctgc tcgactccga cgccggggca
    16081 tacgatgtat aggtgataag tgatgagtga taagtgatga gaaaatcagt ttccttctta
    16141 tcacttatca ctcatcactt atcacggctt ttatgagttt cttcaagcgg caaatacagg
    16201 tcgcgcgggt ggcgggaatt cccgtgcgta tcgactaccg ctggtttctt gtcttcgcgc
    16261 tgtcggcgtg ggtgctggcg gcgagctttc agcagccggc gtcggtgctg acgcagggcg
    16321 tacatctgga cgcgggcgcg gcgtggctgg cgggggtggc gacgacaatc ggcctcttca
    16381 tctcgatctt cggccacgag ctttcgcacg cgctcgccgg ccggatggag ggcatcgaga
    16441 gtgaggagat cgtgctccac ccgttcggcg ggttcacccg gctgcggcgc gagccggaca
    16501 gcccgcgcgc cgagtttcgc atcgcgattg ccgggccgtc ttcgagtttc ctgctggcgc
    16561 tcgtcttctt cctgctgttc gccgccgcgc ggtttgcgaa ctaccgcgcc gtcgccgccg
    16621 gcctctccat cgtcgcctac tggaacctct tcatcgccgt ctcgaacctc ctgcccggct
    16681 acccgctcga cggcggacgc gtgctgcgcg cctttctgtg gaagcgttac ggccggctcg
    16741 acgaggcgac gcgcatcgcc tcgctcggcg ggcagctcat cgccggcacg ctcgtcgtct
    16801 tcggcgtcta cttttactgg aaactcaacg acgccttccg cggcctgtgg ctcgtcctca
    16861 tcggactctt cctgtgggac gcggcgcgct cggtctacgg cggccgtcgc cgcggcgcgc
    16921 gcaccgtggc cgacgcgatg ggcgcgcccg tcgccgt
//
LOCUS       DASVES010000270        12265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1365410, whole genome shotgun sequence.
ACCESSION   DASVES010000270 DASVES010000000
VERSION     DASVES010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365410"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VF240_10845"
     CDS             complement(<1..365)
                     /locus_tag="VF240_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175746.1"
                     /translation="MPTCGSGAWVVSLRHCRFKKPNRQVFAVVVSGAFDSSAERHNFE
                     MLPGRRVICLLGSVPLAAWFAAAQIGLICAVAPGASAATPKVLFEAAARSQTPQTLSV
                     PADSPRWELQGQAKAAEYQG"
     gene            537..1334
                     /locus_tag="VF240_10850"
     CDS             537..1334
                     /locus_tag="VF240_10850"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7175747.1"
                     /translation="MTTIGTVESLWRYPVKSMRGVEMPEVFMGFSGIYGDRCYAFKNS
                     AARKGFPYLNANVQQQMLQYRPQFRHPERASRPPNLTEATSIAPGVTPANADAEDMIL
                     DILTPSGEIVSVDDPALLKLLGEGISEKNQLQLARSDRALTDCRPVSLISLSTVRQIE
                     TELGIPIDKRRFRANIYFDFASHGDAFAEDDFVGRRLRIGSKATIMVLERDPRCKMIS
                     LDPDTGEHNPEVLRKVAQAHGNFAGVYCAVLVEGLLKKGDFIELVAN"
     gene            complement(1479..2357)
                     /locus_tag="VF240_10855"
     CDS             complement(1479..2357)
                     /locus_tag="VF240_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7175748.1"
                     /translation="MANRARRLPENVEGDFFVDQSCIDCDACRQIAPATFRDHGDASS
                     VYRQPVAVDEITRALMALTACPTASIGTTRDYDASIGVEAFPELLDGNVYFCGFTSED
                     SFGAWSYLIVRPEERGGNVLVDSPRFTRPLVRKIEAKGGVRTIFLTHRDDVADQEKFA
                     RHFAAARVMHADDGARRLGVETIVEGEREVSLDAELRVIPTPGHTRGHMVLLYADKYL
                     FTGDHLAWSPARQALTAFRTAMWYSWPEQTRSMERLLAHTFEWVLPGHGRIHHDTAPA
                     MREHLRRCVEWMKATP"
     gene            complement(2517..3464)
                     /locus_tag="VF240_10860"
     CDS             complement(2517..3464)
                     /locus_tag="VF240_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7175749.1"
                     /translation="MKRTAFAFAFLLLSYVTTAEVVAQVNPAATPVPSPTPGPAPTPP
                     EWLDVRVRDATADFSGSVSLYAKNLDTGAVYSFNGERQVRTASTIKIAVMIEAFARVA
                     EGKARWADEVVLTKEKKVSGSGILSELSEGLKLSLRDAVNLMMILSDNTATNLVIDAL
                     TADAVNARMAALGLKDVRLMRKVGGGGMSEEGKREENKRFGLGRASPREMVALMEKLE
                     RGEVVSSEASKEMIELMKSEQGRDGIWRSLWRVPKATKGGALDALRSDIGVIYSPKGR
                     IALAVTCDDMPEPQWTIDNPALLLMSRLSEIIIEGLGKR"
     gene            complement(3654..4559)
                     /locus_tag="VF240_10865"
     CDS             complement(3654..4559)
                     /locus_tag="VF240_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7175750.1"
                     /translation="MEAYDAIRRDNRRHFLRRMMLTLALLLVVGVPLLFYAVRRFERA
                     ATFYPVAFDGGPTWRLPRGAEDVWIKTRSGVRLHGWLVRTQRDAPAATVVYFHGNGGN
                     LSYVGWLAEELAGRGFDVLVFDYKGYGRSEGATTDERSLYEDADAVYDYLTVERERAP
                     ERLILYGQSLGTAAVVDLASRRPCGALILESGLSSASDVAALLMPWAPRFLHRMGRNR
                     LDTARKLARVERPVLVAHGEADDIIPVEQGRALYEAAKEPKRLLIIPRAGHNNLVAAG
                     GHAYLDGLAEFIRASVGESDGAPAP"
     gene            complement(4774..6087)
                     /locus_tag="VF240_10870"
     CDS             complement(4774..6087)
                     /locus_tag="VF240_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7175751.1"
                     /translation="MTKKTLMIIVLALCLAAADMSVGRTNDFRQTGGRQQMIAQSAEA
                     LRDKLVAQRRDFHAHPELSNREERTARVVAERLRALGLTDIRTGVARHGVVALLKGAK
                     PGPVVAVRADMDALPIQETIDVPYKSRNPGVKHACGHDVHTTVGLGVAEVLSKMRDEI
                     HGAVKFIFQPAEEGAPAGEEGGARLMIKEGVLENPRPSAIFGLHTEPNLQAGQIGYHS
                     GPAMASSDRFFITVRGKSSHGAQPHRGVDAIVVAAEAIGAIQNVRSRRIDPLEPLVIT
                     VGTIHGGARNNIIAEEVKMEGTMRTLSEEVRERAQALVRETLQNVTAAYGATFELKFE
                     EQTAVTFNEPSLVEKALPVMRSIVGDGNVIAIKPFMPAEDFSYFQRVVPGFYYFLGVG
                     NRAKGITAGWHTPDFDVDEESLVVGVKVMSNTLLDYLERHAGETK"
     gene            complement(6118..7701)
                     /locus_tag="VF240_10875"
     CDS             complement(6118..7701)
                     /locus_tag="VF240_10875"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX7175752.1"
                     /translation="MDKTEKTKNLAPGAPAEPFNLRELLEARGRATPSKTFLVSECDG
                     REFTYAEFDAAVNRTANMLSAHGLAKGDAVSLLMPNSAEYVIAYFACFCLGALAGPVN
                     SLLKPQEMAYAVGDSEARALLYHTEFETHVSELRNATPRLEAAIVFDSVDEATAGQSD
                     ELNERARATRLGPEDDAIIIYTSGTTGKPKGCLLTHANLLANARQIAAWLGFTSSDRL
                     LTVMPLFHMNAVSVTTAAALYAGGSTLVSPRFSATRFWQIVSDYGVTSFGSVATMLSM
                     LLAKYPEGVPEGLNTERLRFAMCGSAPVPAEVLRRFEQTFGCLVVEGYGLSESTCRST
                     FNPPDARRRPGSCGLPIGNEMKIFDEEDREVPDGELGEIVMRGENVFKGYYKNPQATE
                     RAFRSGWFHTGDVGYRDPEGFFFIVDRKSDMIIRGGENIYPREIDEVLYQHAAVAEAA
                     AVGLPDELYGEEVAAFVVLKAGRAATEDEIKVHCRAHLADFKCPKVVHFVEALPKGPT
                     GKVLKRELTRKLKQVGGGG"
     gene            7829..9355
                     /locus_tag="VF240_10880"
     CDS             7829..9355
                     /locus_tag="VF240_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7175753.1"
                     /translation="MREPAGHSHTARARNLMQTYRNFIAGAWVESSSEKTAENVNPAD
                     TGDVLGVVKLSTREEARRAVEAASDAFRAWRATPAPTRGRIVARAARLMEDDRENLAR
                     LLTREEGKTISESRGELQRAINVAEFCAGESRRMNGETIQSELPANFAYTLRHPHGVV
                     ACVTPWNFPVAIPIWKIAPALVAGNTVVFKPATITPATAVRICEIFAEAGTPAGVLNL
                     ILGSGSEAGDEIVNHAAVRAISFTGSNGVGLKLYEQAARRGAKVQCEMGGKNPVVVLE
                     DADLALAVESTAQGAFGSTGQRCTATSRAVVVDEIADAFVERIVARAESMRVGDGAAE
                     GTDVGPIVDEGQFKSVLQYIDIGREDGAELMCGGTVPDGDSSGKGYFVRPAVFDRVTP
                     DMRIAREEIFGPVLSVLRVRDFEEALRVANDSDYGLSSSVFTQDAARIFRFVDEIETG
                     MTHVNSPTTGGEAHVPFGGSKATGIGDREQGSTALDFYTELKVVYVDYTGRKREGNLY
                     "
     gene            9490..10800
                     /locus_tag="VF240_10885"
     CDS             9490..10800
                     /locus_tag="VF240_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7175754.1"
                     /translation="MSKVETKQTQSEAVSKHKEFLFPAVATYYKEPLALVRGEGMYVW
                     DDEGQRYLDCFGGVLTVSVGHANPRVNAAIIEQVQTLQHTSTLYANKPQGDLAEKIAE
                     IAPGRLKKSFFTNSGTEADDMAIHAAKVATGRHEIVVLRHSYSGRSATALSAVGHSTW
                     RPLPAQVAGIVHARAPYCYRCPLKLTYPECGLACAEDIKDVIMTATTGQIAAFMAEPI
                     LGVGGFIVPPEGYFERAAEITREHGGLFIADEVQTGWGRTGDKWFGIEHWNVEPDIIT
                     SAKGLGNGVPIGMTTATPEVADKFPGLTFSTFGGNPVSSAAALAVIRVIEEDNLKHNA
                     AVVGGYLRARLEELKEKYPVVGDVRGMGLMQALEFVKDRETKEPHPQAVIHVFEETRR
                     RGVLIGKGGLYGNVIRTGMPLIADKDHVDELCRALDAALAGLPS"
     gene            10917..>12265
                     /locus_tag="VF240_10890"
     CDS             10917..>12265
                     /locus_tag="VF240_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175755.1"
                     /translation="MRFPSPTPTLGHPLAALRHFSNGLMLAPAALLLLLTLAPAATTQ
                     AQALATAPIIDREHFFGDPEIAGAQISPDGQFIAFIKPYKGTRNVWVKRTDEPFSAAR
                     PVTADTARPIPGYFWTHDGKFILFVQDKAGDENYNVYAVNPADKPAAGQDAPAARNLT
                     DAKGVRAALYAVPKTEPDVMYIGLNDRDQAWHDLYRVKISTGERTLLRQNTDRITGWI
                     FDHKGQLRLATRSAENGDTEVLRVDDKAFTKVYSCSVAEACGPVRFHKDNQRVYMITN
                     KGDADLMRLALFDPATSKIEPVESDPLNRVDFGAPIFSDLSDELVGTAYEDERTRIYW
                     KDKSFEADYKLLQQKLPGRDINFGSSTKNERLWLISASSDREPGETYLFDRTTKKLTK
                     QYSVFDKLPREHLAEMKAIRFKSSDGLEVPAFLTLPKGVAAKNLPLVVVPHGGPWYR"
BASE COUNT         2205 a         3999 c         3879 g         2182 t
ORIGIN      
        1 ccttgatatt cagccgcctt ggcttgtcct tgcagctccc agcgcgggga gtccgcgggg
       61 acggagagcg tctgcggtgt ctgggaacga gcggcggctt caaagagcac tttgggcgtc
      121 gccgcggagg cccccggcgc gacggcacaa atgagaccta tctgcgccgc tgcgaaccac
      181 gccgcgagcg gaacggaacc caaaaggcaa atcactcgtc ttcccggcag catttcaaag
      241 ttgtgtcttt ccgctgaaga atcaaaggcg cctgagacaa ccacagcgaa cacttgccgg
      301 ttcggttttt tgaaacgaca atggcgcaag ctgacgaccc aagccccgga gccacacgtc
      361 ggcattctta atgtatttct tttcgcggaa gcactgtttt gtgtttgaac ggcccgaagc
      421 ggtgacgaaa cggaagcggt cctgctggcg ggacattctc attgaagcgc tactcgggtg
      481 acgccgggcg ttagatgctt ctacgcaatg ctggaaagct aaaatgagat tttgatatga
      541 caactatcgg gacagtcgaa agcttatggc gttatccggt taaaagtatg cgcggcgtgg
      601 aaatgcccga agtgtttatg ggattttcgg gcatctatgg cgaccgctgc tacgccttca
      661 aaaattccgc cgcccgcaaa ggcttccctt atttgaacgc caatgtgcag caacagatgt
      721 tgcagtatcg tccgcaattc cgtcacccgg aacgcgcctc aagaccgccg aatttgactg
      781 aagcaacgag catcgcaccc ggtgttacac ctgccaacgc cgacgcggaa gatatgattt
      841 tagacatcct tactccatca ggcgaaatcg tttccgttga tgacccggcg cttctcaaac
      901 tactcggcga aggaatcagc gaaaagaatc aactgcaact cgcgcgctca gaccgcgctt
      961 taaccgattg tcgcccggta tcgctcatca gcctgtcaac cgttcgacag attgaaacgg
     1021 aattgggcat tccgattgat aaacggcgtt tccgagcaaa catctatttc gatttcgcgt
     1081 ctcacggcga cgcatttgcc gaagatgatt ttgtcggacg ccgtctgcgt atcggttcaa
     1141 aggcgacgat tatggttctt gagcgcgacc cgcgctgtaa gatgatttcg ctcgacccgg
     1201 acacgggtga acacaacccg gaagttttac ggaaggtcgc ccaggcacac ggtaactttg
     1261 ccggagttta ctgtgcggta ttggtcgaag gcttattgaa aaaaggcgat ttcatcgaac
     1321 tcgtagccaa ttgattttcg attatcgttc gtcgttttct ttccgcggta tgtggatatg
     1381 gggcgggggg gttggacaac cacgcgaggc cggtgcgggc ctccgggcgc ctccagaagt
     1441 gaatccttac gggtgagtca actattttta cgccctgctc agggcgtcgc cttcatccac
     1501 tcgacgcagc ggcgcaggtg ctcgcgcatg gcgggggccg tgtcgtggtg aatccgcccg
     1561 tgtccgggca agacccactc gaacgtgtga gccagcagcc tctccatcga gcgcgtctgc
     1621 tcgggccacg agtaccacat ggctgtgcgg aaggccgtga gcgcctgccg cgcgggcgac
     1681 cacgcgaggt ggtcgcccgt gaagagatac ttgtcggcgt agagcagaac catgtggccg
     1741 cgcgtgtgac cgggcgtggg gatgacgcgc agctccgcgt cgagggagac ttccctctcg
     1801 ccttcgacga ttgtttccac cccgagccgg cgcgcgccgt cgtccgcatg catcacgcgc
     1861 gcggcggcga agtggcgcgc gaacttttcc tgatcggcca cgtcgtcgcg gtgcgtgagg
     1921 aagatggtgc ggacgccgcc cttggcttcg atctttcgga cgagcgggcg cgtgaaccgt
     1981 ggcgaatcga cgaggacgtt gccgccgcgc tcctcggggc ggacgatcag gtaactccac
     2041 gccccgaagc tgtcttcgga agtgaagccg cagaagtaga cgttgccgtc aagcaactcg
     2101 gggaaggctt cgacgccgat gctcgcgtcg tagtcgcgcg tcgtgcctat cgaggccgtg
     2161 gggcacgccg tcagggccat cagggcgcgc gttatttcgt caacggcgac gggctggcgg
     2221 tagacgctcg acgcgtcgcc gtggtcgcgg aaggtcgcgg gcgcgatctg ccggcaggcg
     2281 tcgcagtcga tgcaggactg atcgacgaag aaatcgccct cgacgttctc cggcagacgc
     2341 cgcgctcggt tagccatgcg gagagtttaa agttcaaagc tcaaagttca aagtcaacat
     2401 tagttcaaag ttcaaagttc aaagttcaaa gttcaaaggc cggccacgcc gggcgcgacg
     2461 caacgcccgc gacggacgcc acagttgact ttgaactttg aactttgaac tttgaactac
     2521 ctcttcccca acccttcgat gatgatttcg gagaggcgcg acatgagcag cagggcgggg
     2581 ttgtctatcg tccattgtgg ctcgggcatg tcgtcgcagg tgacggcgag ggcgatgcgg
     2641 cctttgggcg agtagatgac gccgatgtcg ctgcggagcg cgtcgagcgc gccgcccttc
     2701 gtcgccttcg gcacgcgcca gagtgagcgc cagatgccgt cgcgcccctg ctcgctcttc
     2761 atcagctcga tcatctcctt cgacgcttcg gagctgacga cctcgccgcg ctccaacttc
     2821 tccatgagcg cgaccatttc gcgggggctg gcgcggccga ggccgaagcg tttgttctcc
     2881 tcgcgcttcc cctcctcgct catgccgccg ccgcccacct tccgcatcag ccgcacgtct
     2941 ttcagcccga gcgcggccat gcgcgcgttg acggcgtcgg ccgtgagcgc gtcgatgacg
     3001 agattggtcg ccgtgttgtc gctgaggatc atcatcaggt tgacggcgtc gcgcagggag
     3061 agcttcagcc cctcggacaa ctccgacagg atgcccgagc cgctgacctt cttctccttc
     3121 gtcagcacga cctcgtccgc ccaacgcgcc ttcccctccg ccacacgcgc gaaggcttcg
     3181 atcatcacgg cgattttaat ggtgctcgcc gtgcgtacct gccgctcgcc gttgaaagag
     3241 tagaccgcgc ccgtgtcgag attcttcgcg tacaggctga cggagccgct gaagtcggcg
     3301 gtcgcgtcgc ggacgcgcac gtcgagccac tcgggcggcg tcggcgcggg gccgggcgtg
     3361 ggagacggga caggagtagc ggccggattg acctgtgcga ccacttccgc agtcgtgaca
     3421 tatgaaagca atagaaacgc aaatgcgaag gccgttcttt tcatcgttgg caacacactc
     3481 ctccttcgag tcagcttgag cggcggacaa atttgtaggg gcagggcttg tccgcggccc
     3541 gcgtcatcga tgtgggcacc gttgaattcg gcggaacttg acggacaggg acaagcccca
     3601 cccctacagt ttttcaatgc gttcttaacc cactcgtcgg cgcgcacccc ggctcagggc
     3661 gcgggcgcgc cgtcggattc gcctacggag gcgcggatga actcggcgag gccgtcgagg
     3721 tacgcgtggc cgcccgccgc gacgaggttg ttgtgtccgg cgcgggggat gatgaggagg
     3781 cgcttcggct ccttcgcggc ttcgtagagc gcgcggccct gctcgacggg gataatgtcg
     3841 tcggcctcac cgtgcgcgac gaggacgggg cgctcgacgc gcgcgagctt gcgcgccgtg
     3901 tcgaggcgat tcctccccat gcgatgcagg aagcgcggcg cccacggcat caacagggcc
     3961 gccacgtcgc tcgccgaact caggcccgac tccaggatga gcgcgccgca cggccggcgc
     4021 gaggcgagat cgacgacggc cgccgtgccg agcgactgtc cgtagaggat gagccgttcg
     4081 ggcgctcgct cccgctcaac cgtgaggtaa tcgtagacgg cgtcggcgtc ttcgtagagg
     4141 ctgcgctcgt ccgtggtcgc gccctcgctc cggccgtagc ccttgtagtc gaagacgagc
     4201 acgtcgaagc cgcgcccggc cagttcctcg gcgagccagc cgacgtagct gagattgccg
     4261 ccgttgccgt ggaagtagac gacggtggcg gcgggcgcgt ctcgttgcgt gcggacgagc
     4321 cagccgtgga gacgcacgcc gctgcgcgtc ttgatccaca cgtcctccgc gccgcgcggc
     4381 agtcgccacg tcgggccgcc gtcgaaagcg acggggtaga acgtcgccgc ccgctcgaaa
     4441 cgacggacgg catagaagag gagcggcacg ccgacgacga ggaggagtgc gagtgtcaac
     4501 atcatgcgcc ggaggaagtg gcgacggtta tcgcgtctga tggcatcgta tgcttccatc
     4561 tgcggagatg tggctcatac ttgagcattg ggtgcgtcag ctcaccgcag aggcgcagag
     4621 ggcgcagaga ctacgcagag gcttgacgct gacactcgtc gttaagtcaa aggctcaacc
     4681 ctctctgcgc cacctctgcg tcctctgcgc ctctgcggtg agttgactct cacaacactc
     4741 aatgcggtag cgacgacagt ccaacgcgcg gagttacttc gtctccccgg cgtgtcgttc
     4801 gaggtagtcg agcagggtgt tcgacatcac cttgacgccg acgacgagac tctcctcatc
     4861 cacgtcgaaa tcgggcgtgt gccacccggc ggtgatgccc ttcgcgcggt tgccgacgcc
     4921 gaggaagtag tagaagccgg ggacgacccg ctggaagtag gagaaatcct ccgcgggcat
     4981 gaagggcttg atcgcgatga cgttcccgtc gccgacgatg ctccgcatca cgggcagcgc
     5041 cttctcgacg agcgacggct cgttaaaggt gacggcggtc tgctcctcga acttgagttc
     5101 aaaggtcgcg ccgtaggcag ccgtgacgtt ctgaagcgtc tcccgcacca gcgcctgcgc
     5161 gcgctcacgc acttcctcgc ttaacgtccg catcgtgcct tccatcttca cctcctcggc
     5221 gatgatgttg ttgcgcgcgc cgccgtgaat cgtgccgacg gtgatgacca gcggctccag
     5281 cggatcgatc ctccggctgc gcacgttctg gatggcgccg atggcctcgg cggcgacgac
     5341 gatagcgtcc acgcccctgt gcggctgcgc gccgtgagag cttttgccgc ggacggtgat
     5401 gaagaagcgg tcggaggagg ccatcgccgg ccccgagtgg tagccgatct gcccggcctg
     5461 caagtttggc tcggtgtgca ggccgaagat ggccgacggg cgtgggttct ccagcacccc
     5521 ctccttgatc atcagacgcg ccccgccctc ctcgcccgcc ggcgcgccct cctccgccgg
     5581 ctggaagatg aacttcacgg cgccgtgaat ctcgtcgcgc atcttgctca ggacttcggc
     5641 cacgccgagg ccgacggtcg tgtgtacgtc gtgcccgcag gcgtgtttga cgccggggtt
     5701 ccgcgacttg tacggcacgt cgatagtttc ctgaatcggc agcgcgtcca tgtcggcgcg
     5761 cacggcgacg acggggccgg gctttgcgcc cttcagcaac gcgacgacgc cgtgtcgggc
     5821 gacgccggtg cggatgtccg tcagaccgag cgcgcgcaga cgctccgcga cgacgcgcgc
     5881 cgtgcgctcc tctcgatttg aaagctccgg gtgcgcgtgg aagtcgcggc gctgcgcgac
     5941 gagcttatcg cgcagggctt cggccgactg ggcgatcatt tgctggcgcc cgcccgtctg
     6001 tcggaagtca ttggttcgac cgacactcat gtcagccgcc gcaaggcaga gcgcgagcac
     6061 gatgatcatc agcgtctttt ttgtcattct cactccctcg atgtcgccac tgcggcctca
     6121 gccgcctcca cccacttgtt ttagtttgcg cgtcagctcg cgcttcaaga cctttcccgt
     6181 cggccccttc ggcagcgcct cgacgaagtg gacgaccttc gggcacttga agtcggcgag
     6241 gtgcgcgcgg cagtgtactt taatttcatc ttccgtggcg gcgcgtcccg ccttgaggac
     6301 gacgaaggcg gcgacctcct cgccgtacag ctcgtcgggg agtccgacgg cggcggcttc
     6361 ggcgaccgcc gcgtgctggt acaacacttc gtcgatctcg cgcgggtaga tgttctcgcc
     6421 gccgcggatg atcatgtctg acttgcggtc aacgataaag aagaagcctt cggggtcgcg
     6481 gtagcccacg tcgccggtgt ggaaccagcc ggagcggaag gcgcgctcgg tggcctgtgg
     6541 gtttttgtag tagcccttga aaacgttttc gccgcgcatg acgatctcac cgagttcgcc
     6601 gtcgggcact tccctgtcct cttcgtcgaa gattttcatc tcgttcccga tgggcagacc
     6661 gcacgagccg ggacggcggc gcgcgtcggg cgggttgaag gtcgagcgac aggtcgattc
     6721 cgaaagcccg tagccctcga cgacgagaca gccgaaggtc tgctcgaaac gccgcagcac
     6781 ttccgccggc accggcgccg agccgcacat ggcgaaacgc aggcgctccg tgttcagacc
     6841 ctcggggacg ccttcggggt acttcgccag aagcatcgag agcatggtgg cgacggagcc
     6901 gaaggaggtg acgccgtagt ccgagacgat ctgccagaag cgcgtcgcgg agaagcgcgg
     6961 gctgacgagc gtcgagccgc cggcatagag ggccgcggcg gtcgtcaccg acacggcgtt
     7021 catgtgaaag agcggcatca cggtgagcag ccggtcggag gacgtgaagc cgagccaggc
     7081 ggcgatctgc cgcgcattgg cgagcaggtt ggcgtgcgtg aggagacagc cctttggttt
     7141 gcccgtcgtc cccgaggtgt agatgatgat ggcgtcgtct tcggggccga ggcgggtcgc
     7201 gcgcgcgcgt tcgttcaatt cgtcgctctg cccggcggtc gcctcgtcca cgctgtcgaa
     7261 gacaatcgcc gcttcgagtc gcggcgtcgc gtttcgcaac tcgctgacgt gcgtctcgaa
     7321 ctccgtgtgg tagagcagcg cccgcgcctc ggagtcgccg accgcgtagg ccatctcctg
     7381 cggcttgagc agagagttga ccgggccggc gagcgcgccc aagcagaagc aggcgaagta
     7441 ggcgatgacg tactcggcgc tgttcggcat caggagactg acggcgtcgc ctttggccaa
     7501 gccatgcgcc gagagcatgt tggcggtgcg gttgacggcc gcgtcaaact ccgcgtaagt
     7561 gaactcccgc ccgtcgcact cggaaacgag gaacgtcttc gagggcgtgg cccgtccgcg
     7621 cgcttcgagg agttcgcgca aattaaacgg ctcggcgggt gcgccgggcg cgaggttttt
     7681 tgtcttctcg gttttgtcca ctgtgctata tttacgcgtt cggtcaaaga ccccgcgcgc
     7741 gacttcaaga ctttatcagg agcgccgcta gcttagcgaa aacgcttccc gttagacaaa
     7801 ctccggcgcc cgtcgcggac ttcctcgcgt gcgagaaccg gcgggccact cccacacggc
     7861 gcgcgcccga aacctgatgc agacttaccg aaatttcatc gccggcgcgt gggtcgaatc
     7921 ctcctccgag aagaccgccg agaacgtcaa cccggccgac acgggcgacg tgctcggggt
     7981 ggtcaaactc tccacgcgcg aagaagcgcg ccgcgccgtg gaggccgcct ccgacgcctt
     8041 ccgcgcgtgg cgcgccacgc ccgccccgac ccgcggccgc atcgtcgcgc gcgccgcgcg
     8101 tctgatggag gatgaccggg agaacctcgc gcgcctgctg acgcgcgagg agggaaagac
     8161 catctccgaa tcgcgcggcg agttgcagcg cgccatcaac gtcgccgagt tctgcgcggg
     8221 cgagtcgcgg cgcatgaacg gcgagaccat ccaatcggag ctgccggcga acttcgccta
     8281 caccctgcgc cacccgcacg gcgtcgtggc ctgtgtcacg ccgtggaact tccccgtcgc
     8341 catccccatc tggaagatcg cgcccgcgct cgtcgccggc aacacggtcg tcttcaagcc
     8401 ggccaccatc acgcccgcca cggcggtgcg catctgtgaa atcttcgccg aggcgggaac
     8461 accagcgggc gtgctcaatc tcatcctcgg ctccggctcc gaagccggcg acgagatcgt
     8521 caaccacgcg gccgtgcgcg ccatctcgtt taccggctcg aacggcgtcg gcctcaaact
     8581 ctatgagcag gcggcgcggc gcggcgcgaa ggtgcagtgt gagatgggcg gcaagaaccc
     8641 cgtcgtcgtg ctcgaagacg ccgacctcgc gctcgccgtg gaatcaacgg cgcagggcgc
     8701 cttcggctcg acgggccaac gctgcacggc gacgagccgc gccgtcgtcg tcgatgaaat
     8761 cgccgacgct tttgtggaac gcatcgtcgc gcgcgccgaa agtatgcgcg tcggcgacgg
     8821 cgcggccgag gggacggacg tggggccgat tgtggacgag gggcagttca agtcggtgct
     8881 tcaatacatc gacatcgggc gcgaggacgg cgctgaactc atgtgcggcg gcacagtgcc
     8941 cgacggcgac tcctccggca aaggttactt cgtccgcccc gccgtcttcg accgcgtcac
     9001 gcccgacatg cggatcgccc gcgaggagat tttcggcccc gtgctctcgg tgctgcgcgt
     9061 gcgcgatttc gaggaagccc tgcgcgtcgc caacgacagc gattacggcc tgtcgtcttc
     9121 ggtcttcaca caggacgccg cgcgcatctt ccgcttcgtc gatgagatcg agaccggcat
     9181 gacgcacgtc aactcgccga ccaccggcgg cgaggcgcac gtccccttcg gcggcagcaa
     9241 ggccaccggc atcggcgacc gcgagcaagg ctcgaccgcc ctcgacttct acaccgagct
     9301 aaaggtcgtc tacgtcgatt acaccggccg caagcgcgaa ggcaacttat actgattact
     9361 gatggaagga gcatcaacat ggatggacag gatgttcagg atgagatgaa ttgaaatctc
     9421 aaatatcaaa tcagcccttt cgttatcctg aacatcctgt ccatccatgt tgatattgcg
     9481 tgaattgtta tgagcaaagt tgaaacaaag cagacgcagt ccgaggccgt gagcaagcac
     9541 aaagaatttc tcttccccgc cgtcgccacc tattacaagg agccgctggc gctcgtgcgc
     9601 ggcgagggga tgtacgtctg ggacgacgag ggccaacgct acctcgactg cttcggcggc
     9661 gtgctcaccg tcagcgtcgg ccacgccaac ccgagggtca acgcggcgat catcgagcag
     9721 gtgcagacgc tccagcacac ctccacactc tacgccaaca agccgcaggg cgacctcgct
     9781 gagaagatcg ccgagattgc gccgggccgc ctgaaaaaat ccttcttcac caacagcggc
     9841 accgaggccg acgacatggc catccacgcc gccaaggtcg ccacggggcg gcacgagatc
     9901 gtcgtgttgc gccacagcta ttcgggtcgt tcagcaaccg ctttatccgc cgtcggtcat
     9961 tccacgtggc ggccgctgcc cgcgcaggtc gccggcatcg tccacgcacg cgcgccctac
    10021 tgctatcgct gcccgctcaa gctcacctac cccgagtgcg ggctggcctg cgccgaagat
    10081 atcaaggacg tgattatgac ggcgacaaca ggacagatcg ccgccttcat ggccgagccg
    10141 atcttaggcg tcggcggctt catcgtcccg cccgagggct acttcgagcg cgccgccgaa
    10201 atcacgcgcg aacacggcgg cctcttcatc gccgacgagg tgcagaccgg ctggggccgc
    10261 accggcgaca agtggttcgg catcgagcac tggaacgtgg agccggacat catcacttcg
    10321 gcgaagggtc tcggcaacgg cgtccccatc ggcatgacga cggccacgcc cgaggtcgcc
    10381 gacaagtttc ccggtctcac cttctcgacc ttcggcggca accccgtttc ctcggcggcg
    10441 gcgctggcgg tcatccgcgt catcgaagag gataatctga agcacaacgc ggcggtggtc
    10501 ggcggctacc tgcgcgctcg tctcgaagag ttgaaggaga agtacccggt cgtcggcgac
    10561 gtgcgcggca tggggctgat gcaggcgctg gagttcgtca aagaccgcga gacgaaggag
    10621 ccgcatccgc aggccgtcat ccacgtcttc gaggagacgc gccggcgcgg cgtcctcatc
    10681 ggcaagggcg gtctctacgg caacgtcatc cgcacgggga tgccgctcat cgccgacaaa
    10741 gatcacgttg acgagttatg ccgggcgctc gacgcggccc tcgccgggct gccttcgtaa
    10801 ccacgtcggc cactgaaaca cgctcaaacg gccgcgcgta aaaacgtcgt cttcgccgcc
    10861 gggctgccgc tcggcggcgg ccgttcacac cagatataga aatgaggttt acagaaatgc
    10921 gctttccctc gccaactccg acgctcgggc acccgctcgc cgcgcttcgc cacttctcaa
    10981 acggactcat gctcgccccg gcggcgctcc ttctcctgct cacgcttgcg cccgcggcga
    11041 cgacgcaggc gcaggcgctt gcgacggcgc cgatcatcga ccgcgaacac ttcttcggcg
    11101 accccgaaat cgccggcgcg cagatttcgc ccgacggtca gttcatcgcc ttcatcaagc
    11161 cctacaaagg cacgcgcaac gtctgggtca agcggacgga cgagccattt tcggccgccc
    11221 gccccgtcac cgccgacacc gctcgcccca tccccggcta cttctggacg cacgacggca
    11281 agttcatcct cttcgtgcag gacaaggccg gcgacgagaa ttacaacgtc tacgccgtca
    11341 accccgccga caaacccgcg gccgggcagg acgcgcccgc cgcgcgcaac ctgacggacg
    11401 ccaagggcgt ccgcgccgcc ctctacgccg tgcccaaaac cgagccggac gtgatgtaca
    11461 tcggtctcaa cgaccgcgat caggcgtggc acgatcttta ccgcgtcaaa atctccaccg
    11521 gcgagcgcac gctcctgcgc cagaacaccg accgcataac gggctggatt ttcgaccaca
    11581 agggccagct tcgtctcgcc acgcgctcgg ccgagaacgg cgacaccgaa gtcctgcgcg
    11641 tggacgataa ggctttcacc aaagtctatt cgtgcagcgt cgccgaggcg tgcggccccg
    11701 tgcgctttca caaggacaac cagcgcgtct acatgatcac gaacaagggc gacgccgacc
    11761 tgatgcgtct cgccctgttc gacccggcga cgagcaagat cgagccggtc gagtccgacc
    11821 ctttgaaccg cgtggacttc ggcgccccca tcttctccga tttgtcggac gaactcgtcg
    11881 gcaccgccta cgaggacgag cgcacgcgga tttactggaa ggacaaatcc ttcgaggccg
    11941 actacaagct gcttcaacag aaactaccgg gacgcgacat caacttcggc tcctcgacga
    12001 agaacgagcg cctctggctc atctctgcct caagcgaccg cgagccgggc gagacttatc
    12061 tcttcgaccg cacgacgaag aagctcacga agcagtacag cgtcttcgac aaactcccgc
    12121 gcgaacacct ggccgagatg aaggccatcc gcttcaagtc gtccgacggc cttgaggtgc
    12181 cggccttcct caccttgccg aagggtgtgg cggcgaagaa cctgccgctc gtcgtcgtcc
    12241 cgcacggcgg cccgtggtat cgcga
//
LOCUS       DASVES010000271         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1363328, whole genome shotgun sequence.
ACCESSION   DASVES010000271 DASVES010000000
VERSION     DASVES010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363328"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1093)
                     /locus_tag="VF240_10895"
     CDS             complement(170..1093)
                     /locus_tag="VF240_10895"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX7175756.1"
                     /translation="MATNNWPSLPLNEWQDTYATLHMWTQVVGKIRLALTPLVNHWWN
                     VPLYVSARGLTTSAMHYHDRTLEIEFDFIDHLLRITCSDGTTRTLPLEPRTVADFYRE
                     VMRLLGELGVEVKIWTTPVEIPDPVPFEEDTAHASYDPEYAQRFWRILSLSARVLEEF
                     RARFVGKCSPVHFFWGSFDLAVTRFSGRTAPERPDADSITREAYSHEVISHGFWPGAG
                     PLGAAAFYSYTAPEPPGLKEAAVRPTSAFYSNELSEFLLMYDDARQSDSPEAALMEFL
                     QSTYEAGAGLANWDRAALERPLAFKKGIKMN"
     gene            complement(1210..2580)
                     /gene="chrA"
                     /locus_tag="VF240_10900"
     CDS             complement(1210..2580)
                     /gene="chrA"
                     /locus_tag="VF240_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX7175757.1"
                     /translation="MADTTTTSSLPRVPFFEALRLWAKIGFLSFGGPAGQIALMHREL
                     VERRRWISEARFLHALNYCMLLPGPEAQQLAIYIGWLMHRTWGGIVAGALFVIPSIFI
                     LLALSYVYTAYGNLPAVAGALSGFKPVVVAIVVEAVLRIGGRALKRRTHFVIAALSFV
                     AIYFLGLPFPLIVLASGVVGLAVTQLRPLDSETRNVPSSEADKKTVGAGDEEALPLVI
                     DDEAPPPPHTLPSRRRALKIMALGFFLWAVPLGVVAWWRGWDGLHAQVYRFFTKAALV
                     TFGGAYAVLAYVTQTVTAAPYDWITPPQAVDGLALAETTPGPLIMVLQFVGFVAGWNH
                     HPADMSAAASGVASALVTTYVTFLPCFIFIFVGAPYIEVLRGNRRLTGALSGITACVV
                     GVILNLAILVGLAVIWPKGFASGPDWGAALLSAAAFVALIRLKADVLLVVVAGGLIGL
                     VRSLAV"
BASE COUNT          554 a          791 c          970 g          469 t
ORIGIN      
        1 atacaacatt aactgcggcc acggctcgga aagcatgcgg ctcgctcaag tgacaggagg
       61 ttggacagga tttacaggat taacagggtg aagaaagtgc tgataacaga tgtcagattt
      121 gagatttgag gtttcaaatc ttctcatcct gtcagtcctg ttaatcctgt caattcatct
      181 taattccttt tttgaacgct aacgggcgct ccagcgcggc gcgatcccag ttcgcaagac
      241 cggcgccggc ttcgtatgtg ctttggagaa attccatgag cgcggcttcg ggcgagtccg
      301 actggcgagc gtcgtcgtac atcagaagaa attcggaaag ctcgttgctg tagaaggccg
      361 aggtcggacg gacggccgcc tctttgagac ccggcggctc cggggcggtg taggagtaga
      421 aggccgcggc cccgagcggg ccggcgccgg gccagaagcc gtggctgatg acttcgtgcg
      481 agtaagcttc gcgggtgatg gagtcggcgt cggggcgttc gggcgccgtg cggccgctga
      541 agcgcgtcac ggcgaggtcg aaactgcccc agaagaagtg tacgggactg cacttgccga
      601 cgaagcgcgc gcggaactct tcgagcaccc ttgccgagag cgagaggatg cgccagaagc
      661 gttgggcgta ctccgggtca taggatgcgt gtgccgtgtc ctcctcgaag ggcacggggt
      721 cggggatttc gacgggcgtc gtccaaatct tcacctcgac gcccagttcg ccgagaagtc
      781 tcatcacttc gcggtagaag tcggcgacgg tgcgcggttc gagcggaagg gttctggtcg
      841 tgccgtcgct gcacgtgatg cggagcaggt ggtcgatgaa gtcgaactcg atctcgaggg
      901 tgcgatcatg gtagtgcatc gcggaggtcg tgagtccgcg ggcggagacg tagagcggca
      961 cgttccacca gtggttgacg aggggcgtga gcgcgaggcg gattttgccc acgacctgtg
     1021 tccacatgtg gagcgtcgcg tatgtgtcct gccactcatt gagcggaaga gacggccagt
     1081 tgttagtcgc catgtgtgtc accctccgaa gtttgtcaga tcaatgttga tttgagtgta
     1141 ctgtaggtcg gactcaccgc ggaggctcag cggtgaatct atatgcccct cactaatgtg
     1201 aacatcaccc tagaccgcca gcgagcgcac gagaccgatg aggccgcccg cgacgacgac
     1261 cagaagcacg tcggccttga ggcggataag cgcgacgaag gcggcggcgc tcaacagggc
     1321 cgcgccccag tcggggccgc tcgcgaagcc tttgggccag atgacggcga ggccgaccag
     1381 tatcgcgagg ttgaggatga cgccgaccac gcaggcggtg atgccgctga gcgcgcccgt
     1441 gaggcggcgg ttgccgcgca acacctcgat gtagggcgcg ccgacgaaga tgaagatgaa
     1501 gcaggggaga aaggtcacgt aggtcgtgac gagcgccgag gcgacgccgc tggcggcggc
     1561 gctcatgtcg gccgggtggt ggttccagcc ggcgacgaag ccgacgaact ggagcaccat
     1621 gatgagaggg ccgggagtcg tctcggcgag ggcgagtccg tcaaccgcct gcgggggcgt
     1681 gatccagtcg tagggcgcgg ccgtcaccgt ctgcgtcacg taggcgagca cggcgtaagc
     1741 gccgccgaac gtgacgaggg ccgccttcgt gaagaagcga tagacctgcg cgtgcagtcc
     1801 gtcccagccg cgccaccacg cgacgacgcc gagcgggacg gcccacagaa aaaatccgag
     1861 ggccataatc ttcagcgcgc gacggcgcga cggaagagtg tgcggcggcg gcggtgcttc
     1921 gtcgtcgatg acgagcggca gggcttcttc gtcgcccgcg cctacagtct ttttgtccgc
     1981 ctcacttgaa ggcacattcc gcgtctctga atccagcggg cgcaactgtg taacggcgag
     2041 tccgacaacg ccggacgcga ggacgatgag cgggaagggg aggccgagga agtagatggc
     2101 gacgaaggag agggccgcaa tcacgaagtg cgtgcggcgt ttgagagcgc gcccgccgat
     2161 gcgcaggacg gcctcgacga cgatggcgac caccaccggc ttgaaaccgg acaaagcgcc
     2221 ggccacggcc ggcaggttgc cgtatgccgt gtagacgtag gagagcgcga gcagaatgaa
     2281 gatcgaaggg atgacgaaga gtgcgccggc gacgatgccg ccccacgtgc ggtgcatcag
     2341 ccagccgatg taaatggcga gctgctgcgc ttcgggtccg ggcagcagca tgcaatagtt
     2401 caatgcatgc aagaaacgcg cttcgctgat ccagcgtcga cgctcgacca gttcgcgatg
     2461 catgagggcg atttgccccg ccggtccgcc gaagctcaag aaaccgatct tggcccacag
     2521 gcgcagggct tcgaaaaacg gcacgcgtgg aagggaggag gtcgttgttg tgtctgccat
     2581 cgcgccgaat ggtagctcac tggcgtgtcc ggctgatgtc aggtcatatt gggtccccga
     2641 aaaaaaggcg gcgccgactc gcagatcgac gccgctccgg ggtaactcca gctcgttact
     2701 ggcggacctt cgcggtctcc ttcaccagcg ttgcactcgc gtcatcgccg tccagcgacc
     2761 aggagaacaa acctccgaga ccca
//
LOCUS       DASVES010000272        24715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1566037, whole genome shotgun sequence.
ACCESSION   DASVES010000272 DASVES010000000
VERSION     DASVES010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 24715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24715
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566037"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VF240_10905"
     CDS             <1..299
                     /locus_tag="VF240_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175758.1"
                     /translation="PRNIKANPSPSDSDGARARSFLESKGYRMAGDDILMQRLVHLVD
                     EARRNELMVIYIEDLGGMGLTAADFAGGGSAAARWPDISKGLLERAAGGMKISR"
     gene            362..763
                     /locus_tag="VF240_10910"
     CDS             362..763
                     /locus_tag="VF240_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175759.1"
                     /translation="MADSTNNSRRDFHLSLGVFALIVASALCGLALDANWWKFLRVLL
                     AFSTYSTILLLLFRRETSGPLPLWPFALAAGAAELASGWLRPNATPLLSPSVAPVAAL
                     LIGGVQWLTLRAWRRLHGRITAPRHAASEHG"
     gene            complement(867..2270)
                     /locus_tag="VF240_10915"
     CDS             complement(867..2270)
                     /locus_tag="VF240_10915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7175760.1"
                     /translation="MATLSFWGGVGTVTGSKYLIEHGKSRVLVDCGLFQGLKEWREKN
                     WEPLPFEASSLDAVVLTHAHVDHTAYLPRLVALGYDKPVYCTHGTADLVKLLLPDSAR
                     IQEEDADYRNRHKLTRHNPALPLYTEQDARAALKLLRPLENKAEGVDIAKGVRLSLHI
                     AGHILGSSLVLLELEGAGADGAGRRVLFSGDLGHFDQPIIRDPDPPPACDYLLVESTY
                     GDRLHEEIDPKTKLARIINEAVERDGPVLIPAFAIGRTQELIYFLRELEEEKRIPILP
                     VRVDSPMAAAATQAYGQRKEEHDEDYASVLARRRHPLRTHTMLAASTREESKRLNEES
                     GTRIIISASGMMTGGRVMHHARRMLPDPNATVVFVGYQAAGTTGRLVLDGARSVRIMK
                     SDVPVRCRVERVEAMSAHADWKEVLRWLEPIKTAPRRTFTTHGEPEAAAAMAAHINER
                     YGWRVDAPQYGQTVELE"
     gene            complement(2503..3663)
                     /gene="sucC"
                     /locus_tag="VF240_10920"
     CDS             complement(2503..3663)
                     /gene="sucC"
                     /locus_tag="VF240_10920"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX7175761.1"
                     /translation="MKIHEYQGKELLGKYGVAVPRGIVARTPEEAFEAAKELGAEVVV
                     VKAQIHAGGRGKGGGVKLARSPEEARDVASQILGMNLVTHQTGPEGREVRVVLIEEGL
                     PIDKEFYLGIVLDRASGRSVFMASSAGGMDIEEVAEHSPEKILKETIDPAVGFRSFQA
                     RKLAFGLGLPASLTNQAVKFMQTLYSAYEQMDASLMEINPFLLTKDGRLIALDAKVNF
                     DDNALYRHPDFRDLRDTHEEEPLEIEASKFDLNYIKLDGNIACMVNGAGLAMATMDII
                     KLAGGEPANFLDVGGGASQERVEAAFRILLADEHVRAVLINIFGGIVRCDMVANGVVE
                     AAKNLGVKVPVVVRLEGTNVEEGQRVIRESGMNFTVAQGMKDAAEKVVALAG"
     gene            4224..4649
                     /locus_tag="VF240_10925"
     CDS             4224..4649
                     /locus_tag="VF240_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7175762.1"
                     /translation="MSEVIVTSLSNLQNEVRYGDARTLITDEPASVGGNDAGPDPYTL
                     LLGALGSCISMTVSMYARRKGWPLERVVVRLRQQRIHARDCAECERTQEGFVHRIERA
                     VTVEGDLTDEQRARLLEIAHKCPVHKTLVSEIVVAELSD"
     gene            4716..5567
                     /locus_tag="VF240_10930"
     CDS             4716..5567
                     /locus_tag="VF240_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175763.1"
                     /translation="MRRAFAALTLAAAFAFVQLACQSAKNQNQPDNTNAARAEATPVA
                     TPAAEHPLRQQLEEEARQSPDDPNSHFNLGNVYFQERRFEEAIAEYQKVILANPNDAE
                     THGQIGNAHFALKRYSEAVESYKRAASANPKNAEFHNLLGNAYVELRQHPEAAEAFRE
                     AARLNPKHPDAFFNLANAYDALGMYKEEVAALQQAVKVNAKDADAYFNLGNAYSKLNR
                     PKEAAGAYQRAVTLNPGDAVARLTLARTYLKTGDRKGAETQHEALKSIDPNSAEQLLR
                     EINVVQP"
     gene            5810..6571
                     /locus_tag="VF240_10935"
     CDS             5810..6571
                     /locus_tag="VF240_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7175764.1"
                     /translation="MKNLPLKIVACLALAVACVLSSSFVSDNVSAQVKQAKPGDRLSP
                     LERELLTEMNLARTQPAEYAAYLEQLKPFFKGNVYTPAGGRSFNTQEGWAAVEEAIRF
                     LRAAKPIAPFNVAEGMCYGAGELVKDQTKSGGTGHKGSDGSFCEQRLMRYGTWAAPVG
                     ENLSYGRQSARERVLELIIDDGFSSRGHRLRIFNAGHKVAGISCGPHTSLGNVCVITM
                     AGGFTARGPAQKAGASKPGASKTGEAPAPSGAVRF"
     gene            6747..7079
                     /locus_tag="VF240_10940"
     CDS             6747..7079
                     /locus_tag="VF240_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7175765.1"
                     /translation="MFDRELKKGSAELLILSLVEDQPRHGYDIGSLIEQRSRGVLRFN
                     VASLYPLLYRLEKRGWILGRWVEKAGQRRRRYYRLTPEGKKILAAQRIGWREFVEAIN
                     RIIGVENA"
     gene            7072..9771
                     /locus_tag="VF240_10945"
     CDS             7072..9771
                     /locus_tag="VF240_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7175766.1"
                     /translation="MPDWKEEVRRRLSGLRLAPTREAEIVEELALHLDDRYEQLLQGG
                     ATKEEAYKSALLDLTENDLLVQELRRVERPAPQEPVAPGARRRTNLLGEFWQDLRYGL
                     RMLLKNPGFTIVAVIALALGIGANSAIFSVVNTVLLRPLPYKDPERLVMVWEDRTKRG
                     YPRDTPAAGNYTDWRDQNQVFEGMAAIADLSFNLTGVGEPERLDGRRVSASLFPLLGV
                     EPQLGRAFLPEEDQPGGNRVVIMSHGLWQRRFGSDMSIIGKPLNLNGESHTVVGVMPP
                     DFQFPSRDDELWAPIAFDPQEATNRRRHYLQIVARTKPGVTPQQAQVELSAIAERLQQ
                     QYPETNTDVGAAVVPLHEQLVGDIKPALLVLLGAVGFVLLVACANVANLLLARASVRQ
                     KETSIRVALGASRSRLIRQFLTESVLLAALGGVAGLLLSLWGVNLLKAFIPENISQAQ
                     SVTIDGSVLAFTLLVSLLTGLVFGLAPAMQTSSFNLNEALKEGGRDSVAGSRGNRLRG
                     LLVITEVALALILLIGAGLLINSFLRLRNVDPGFRADNLLTMSIVLPQLKYPDQTRRS
                     AFYNELVSRVESLPGVKSAAVTNWIPLVLQGDSIGISVEGRPDPGPGQRPSAVTRVVS
                     PDYFRTMNIQLLQGRQFDAQDKVDSPSVAVISETMARRLWPGEDPTGKRITPGAPDSA
                     NPDDWITVIGVVKDVRQFELVAEPKPQMYLTHIQAGFFAPRHLVVSTDVEPLSLAGAV
                     RKTVWDIDKDQPVSNVNTMESVLSESIARQRFSMLLLGIFAAVALVLAAVGIYGVMSY
                     SVAQRTHEIGIRMALGAQRGDVLKLAVGGGLKLVLIGVGVGLVTAFILTRVMSSLLFG
                     VSATDPTTFIIISLVLISAAVLASYIPARRATKVDPMVALRYE"
     gene            complement(9773..10234)
                     /locus_tag="VF240_10950"
     CDS             complement(9773..10234)
                     /locus_tag="VF240_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175767.1"
                     /translation="MMKIAVAICCLLLLAPACATSVEPKPEILGIRLGMSREEAHARL
                     QSIGQLEKEERRQQEVWRLTNDPGYTHLMVAYNKEYTSVRYVTAVANEQGRRVRYADV
                     VDTGRAREENTPASRAYTQEVPARGDQPGFIVKAIGADPTFLKYYSVEKAD"
     gene            complement(10394..12322)
                     /locus_tag="VF240_10955"
     CDS             complement(10394..12322)
                     /locus_tag="VF240_10955"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7175768.1"
                     /translation="MRKSRTLKSQSAFKALGATLGAIFVLTMVFAPLQMMTADAASPV
                     SRTLSPTAAAPVTWTGTAIGGGALNAAPVIGSEALCREGLTCDTLTLNVGGTAADWAG
                     KLVRVKIEWNLPVTDYDLYIHKGSNAGPLVASSGRGLTSPTEPLTIEDTTIDPSASGT
                     GVYTVRAVYYAATTLDQYRGSAAVEAKPFVPPTPAPTPSNATPPTYRNYASPAGIGES
                     AGEPTLGANWNTGAVMFISGLETLRVQFNDSASPATATWADVSAPTTSITTLDPILFT
                     DSDAGASRTNRTFVSQLAGKLSLMAFTDDDGATWTPSQGSGINSGVDHQTLGGGPYAK
                     NADGSLKGGAVQLPGLNAAFYPNAVYYASQDIGLAQIARSDNGGLTFGVAVPMWTLVQ
                     CGGLHGHIKVAPDGTVYVPNKNCGGTQGVAVSEDNGLTWSIRTVPGSTPGDTDPSVGI
                     GADGTVYLAYADGDGHSRVAVSHNRGLNWVNVSDVGAAHGIQNTVFPAAVGGDANRAA
                     VFFLGSTTSGANGRGTDMTFPGTWFGYIATTYDGGQTWVTVNATPNDPVQRGVVCTSG
                     TTCPGSTRNLLDFNDVTVDKQGRALAAYADGCVTPACISGADKNGDGQLDGNDNDGED
                     RATIIRQSGGKPLFAAFD"
     gene            complement(12715..15714)
                     /locus_tag="VF240_10960"
     CDS             complement(12715..15714)
                     /locus_tag="VF240_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7175769.1"
                     /translation="MKRPRKIVAVILVYALCAGAVIAGVSVRRGEGVFLTGTQGVSFI
                     DTSGITVTGTDDLLAYKVNGITTTGADGITVTGTDGITVTGTDGVTYTGANALVARGV
                     DALSVAAADGITVTGTDGITVTGTDGSSSRLDSILLRKAEGITVTGADGITVTGTDGI
                     TAIGADALSIARANGITVTGTDGITVTGTDGITATGTDGRIFSITPGSIHIADADGIT
                     ATGTDGITLNGIDGITVTGTDGITVTGTDGITVTGTDVQQRTGLQSLDPELALLLDRA
                     TDDSHINAAVVYHRQPGDADLADLRLNGITGGTRYLSLPVVAVTATRQQLVNVSRLPA
                     VRSIYGNRTFQTTSDPYLAPAGVERVARDSDLTRHNMGLNVNGRGVTVAVIDTGLDGT
                     HADLAGRVAQNVKLADTQSLSVGFTYPVGVENVPNTDQVYGHGTFVAGMIAGDGARSG
                     GAYAGAAPGAKLVGLSAGDLNLFHVLAGFDYLLTHPDLGVRVVNCSFSANAVYDANDP
                     VNVATRLLTERGINVVFSAGNSGPGLQTLNPYAAAPWVVSVGATDHTGRLAAFSARGR
                     FGGNAPHPTLVAPGVNVVGLRNTGVGVLGASSLTSSRRLPASAAPYYTTATGTSFSAP
                     QAAGVIALMLEANPDLTPAQVRDILQRTATPLAPHYRHEVGAGMLNAHAAVLEAAFPE
                     RLIGVWRATLDRGQVRFVGDTLRQFGGTTQLLSGYETFLNVPENTLLASVQIAWGPIY
                     STNDLGLNLFDPNGVRRASSNQLNLPGLTGKRERVVIKSPATGRWRVGVSNTLGLAGT
                     PQQFSGVLELTRAEYAPLTDLDGLSQSAREEVYQSFRSFVMWPLGSSFRPAFGVTRSD
                     LATALVLGARVPQYTPAAPTYADVLDRSTMLFVESVQAAPGGPLFADATPGASFRPDD
                     MVDRLTAAVAIVRASGLRAEAESRAGALLNVTDAGAIPSALRGYVAVALERGLLNAEG
                     GAFRPQSALARIELAHAMAVIARRASE"
     gene            complement(15936..19286)
                     /locus_tag="VF240_10965"
     CDS             complement(15936..19286)
                     /locus_tag="VF240_10965"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX7175770.1"
                     /translation="MPQRTRSDSTPAPALTQPALEEEKSPRRPTFFLRTKLLPPRPAP
                     ALLSRPRLTERLQSNLSHPVTLVAAPAGSGKTTLVADFVRAHAQQFVWYQLDHTDADP
                     LVFLGYLAHGIRQAVPGFGDVTLSYLRQSADEIAREPARAVDVLLNEVLEEVSQQLVV
                     VLDDYHHLGRDTAVHAVLDRLISYLPDVLHFIVVSRDVPPLALSRLRSQHSLVIIDRQ
                     ELLFTDEETLELFRQVFDLELTPAQLAEYRERTQGWITALQLVRQFAQREAIASGASE
                     TGAPDLTEILRQSEHDIFDYFAEEVFADEQEDVQRFLLRISLLDRVEMDVCALFYPEV
                     NSRLVLPELVRRNVFLTVASDNRGEEYRLHPLFRSFLRRRLSHELGQTGVAAEHARYA
                     DCYLERGGWEQAMRHLIAAEDFERAASVIAERGGHWVAAGALASLVSFVDALPPDATK
                     AHPRALFYRAEVARLRDELDSARSLHTRAASLLRAHNDREGEAEALRSLATIARRYGD
                     IETALAYLDRAVELADEESFVRAKCAGTRGLCLMAKGEWAAAEREFRAALQSAEDHGD
                     EHYARLMTHNLGLPALIRGDFDESLRWLHRTLQYGGNSPPVPQEAAVHLNMARCYLYR
                     GEVAQCEAHLNRALEFSQLFNLSVLQGEVFEAYGNLYRERREVPRALEFYARAARIYK
                     EAGIDLARRELLEEQAMLSLQEGDPAAARSAMDHLVEARTTLKDEMALKTAVLARGRV
                     MLAQGEHAAAQAELGPALSYFREHNLYYYEAQASLALASCARAGEREGDYLMHLRRAL
                     ELAARYDYEYWLRREVEQNPQLFSSEQALKLLPRDLRQNLSSVQARPQLPHAAAVLAV
                     QTAPARQASDLTINMLGLVEIYRDPSHPLAADAWTSKRARDILCFIASRQHRRASKET
                     LIDTFWRDDDLESVEKKFHPTISYIRKALNSNQLIRQNFLLYRDGDYLLNPDFSYRSD
                     VEEFDGLVAEAAAARRRGQTERCGQAYEEAVALYRGEFMQGTHDEWVEEQRSYYRDQY
                     LRMLEKLASAAQEAGEWEHCLSLAQKILRDDPFREEVHCRVMRAHAALGSRGAVREQY
                     ETLRSLLLNELGVEPAQETQGVYRESIK"
     gene            complement(19414..20232)
                     /locus_tag="VF240_10970"
     CDS             complement(19414..20232)
                     /locus_tag="VF240_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7175771.1"
                     /translation="MRIILDKVSVPHENPRVSRPRLLRLLEEGLGSCTSTIIAGRAGT
                     GKTLLATDFARRCAPRVVWYKVDASDGELRIFFRYLVETVSRQRPGFGQKVLSHIDGS
                     PGLEDIPTLAEAFLYELQECQDAPLLIVIDDLHLIYDAAWVVPFFQRLLPLLPPEAHM
                     LIIGRSAPPTPMWRMRSKQTLCLIEEAELAFTPQEAVDLFSSYGLGAGDARAALEQTR
                     GRAAALDKTAMWMSRAEAGIHHVERNSSRRVACGSRVYPSNSQPRRERRRASGR"
     gene            20609..21496
                     /locus_tag="VF240_10975"
     CDS             20609..21496
                     /locus_tag="VF240_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7175772.1"
                     /translation="MLTINNLSKTYPNGVQALKGVSLNIERGLFGLLGPNGAGKSTLM
                     RTLATLQEPDTGQIIFDGLDVVSDPQAHRRQLGYLPQDFGVYPGVSAYDLLEHLAVLK
                     GVADRKARREQLEALLEQTNLWAHRKKAVSGFSGGMRQRFGIAQALLGDPRLIIVDEP
                     TAGLDPEERNRFHNLLSEVSENIVVILSTHIVEDVRQLCPRMAIMAEGRVLRSGAPRK
                     LIAELEGRVWRKTVEKPAVEAYRKSLHVISTQLYEGRTLVHVLHEGRPEADFEAVEPN
                     LEDVYFSILRAPAEARHAA"
     gene            21486..>24715
                     /locus_tag="VF240_10980"
     CDS             21486..>24715
                     /locus_tag="VF240_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7175773.1"
                     /translation="MLLEVIRFEWRYHTRQIAFPVGVLFFLGMGFILPVLGYGPGGTH
                     LNSPFVVMQSVGLLSLLNIFVLTVFCANAVARDAEHGMREIVYATSIGKLRYLSARFV
                     GAMAAALTVFSCTIVGLYLAPMVAKIDPAKLGDPIPLAYLWALLVMALPNMLFAGAII
                     FALAVLTRSVLASYVGSVFLYMLYMVIAMMIDSPLMAGSTPQSPQAMARAAILDPFGI
                     AAFFQQTWYWTPAQRDTQLLSLDGYFLINRLLVMGVAAAILLVTYRYFSFRLAASARP
                     APAETLEPRATVARYRGVPVAPESAAGRWAALRVATLREFRATAASKPFLALLALWLC
                     VAAISVVDVWAEYRTQLYPTTGIMLEAVEIPLAFLTTLMLVYFAAEVVWRERIVRADE
                     IVDATPAPNGVFYLAKGGALILLSWLMTLVPILVGAGYQLSTGYTQLQPEVYLSLFYF
                     TGLPLALLAVVVLLVQTLSPNRYLGIIVGMILGLLMLSPNELGLMHGLVRFGAPPRAP
                     YTEMNGFLAPGVFGLYMVYWGGLAGVLAFITLGLWRRGRAQTLLGRVRELPRRWGRRG
                     LAGAAGCLAVFLSTGGFVFYNTNVLARYETREQSLDWRAAYERTYRSYEHVPQPSVVA
                     VTTTVDLYPSERRFEVAGAYRLENRTAQPLDTVLISLRRELRPEQMELAGARLVRHDP
                     TFGMYVFALPQPLAPGAATELRFRISSPPVIVEATGFSYSVVANGSYLTRHAAFPRLG
                     YVLGYELEDASERRERGLGPPRKGRGLVNDPSSDDHREEWLTVDATVSTSEDQIAIGP
                     GDLVREWRQDGRRYFQYRTAQPATPRFGFVSGRYQVRRENHRGVSVEVYHHPAHGSNV
                     EKMLAVTTRSLDMFGERFGPYPHQHLRIVELPGHWDFGAFALTGLIVWPEDRGFLTDE
                     SRGGEVDLITRRLAHEVSHQWWGHQLYPAQVEGGTMLVETLAKYSELLVLEATQGQGS
                     LPPMLRYEREMYLLSRTNTPFPEPPLMRVVDLDHVYYSKGAIVMSAMRDLIGEDALNR
                     ALRRLLSEHGAASQPATTENLLAALRAEAAPEHHALIDEWVREVSFL"
BASE COUNT         4195 a         7883 c         8210 g         4427 t
ORIGIN      
        1 acccgcgcaa catcaaggca aacccctcac cgtccgactc cgacggcgcg cgtgctcgct
       61 cattccttga gagcaagggc taccgcatgg cgggcgacga catcctcatg cagcgcctcg
      121 tgcatctcgt ggacgaggcc aggcgcaacg agctgatggt catctacatt gaggacttgg
      181 gcggcatggg tttgacggcc gcagacttcg ccgggggcgg gagcgccgcc gctcgctggc
      241 cggatatctc gaagggactg ctggagcgcg ccgccggtgg gatgaagatt tcgcgctgac
      301 gacgcggggc cgcgcgacct acgcgcccta cagtccattc gtttgccaca ggggaagcaa
      361 ggtggctgac tccacaaaca attcccgccg cgactttcat ctgagcctcg gcgtcttcgc
      421 cctcatcgtc gcatcggcct tgtgcggtct cgcgctcgac gcgaactggt ggaagttcct
      481 gcgcgtgctc ctggccttct ccacttattc cacaatcctg ctgctcctct ttcggcgcga
      541 gacatccggc ccgctccccc tctggccttt cgcgctcgcg gccggcgcgg ccgaactcgc
      601 ctccggctgg ctgcgcccga acgcaacccc cctgctctcg ccttcggtcg cgcccgtcgc
      661 cgctcttctc atcggcggcg tccagtggct caccctccgc gcgtggcgcc gcctgcacgg
      721 gcggatcaca gcgccgagac acgcggcgtc ggaacacgga taagacggaa cacggataaa
      781 acggatgaga cggatgaaca cgaatccgtg cccatccgtc tcatccgtct catccgtgtt
      841 ctaacgcacc cgcgagcgtc ccgcactcat tcaagctcga cagtctgccc gtactgcggc
      901 gcatcgacgc gccagccgta gcgctcgttg atgtgcgcgg ccatcgccgc ggcggcctcc
      961 ggctcgccgt gcgtggtgaa ggtgcgtcgg ggcgcggtct taatcggctc cagccagcgc
     1021 aggacttcct tccaatcagc gtgcgccgac atcgcctcca cgcgctcgac gcggcagcgc
     1081 accggcacgt ccgatttcat aatgcggaca ctgcgcgcgc cgtcgagaac gagtcggccc
     1141 gtggtgcccg ccgcctgata accgacgaag acgaccgtgg cgttggggtc gggcagcatt
     1201 cgccgcgcgt ggtgcatgac gcgcccgccc gtcatcatcc ccgacgcgct gatgatgata
     1261 cgcgtgccgc tctcctcgtt cagacgcttc gactcctcgc gcgtcgaggc cgccagcatc
     1321 gtgtgcgtgc gcaggggatg gcggcggcgc gcgagcacgc tggcgtaatc ttcgtcgtgc
     1381 tcctctttgc gctggccgta ggcttgcgtc gccgcggcgg ccatcggcga gtccacgcgc
     1441 acgggcagga tgggaatgcg cttctcctct tcaagctcgc ggaggaaata gatcaactcc
     1501 tgcgtgcggc cgatggcgaa ggccgggatg aggacggggc cgtcgcgctc gaccgcctcg
     1561 ttgatgatcc gcgcgagctt cgtcttcggg tcgatctcct cgtgcagccg gtcgccgtag
     1621 gttgactcga cgagcaggta gtcgcacgcg ggcggcgggt cggggtcgcg gatgatcggc
     1681 tggtcgaagt ggccgagatc gccggagaag agcacgcgcc ggcccgcgcc gtccgcgccc
     1741 gcgccttcga gttcaagcag gacgagactc gacccgagga tgtggccggc gatgtgcaga
     1801 ctcagacgca cgcccttcgc gatatcaacg ccctccgcct tgttttcgag tgggcgtagc
     1861 agtttcagcg ccgcgcgcgc gtcctgctct gtatagagcg ggagcgcggg gttgtgtcgg
     1921 gtgagtttgt ggcggttgcg atagtcggcg tcctcttcct ggatgcgcgc cgagtcgggc
     1981 agcaggagct tgacgaggtc ggccgtgccg tgcgtgcagt agacaggctt gtcgtagccg
     2041 agcgcgacga gacgcggcag ataggccgtg tgatcgacgt gcgcgtgcgt gagcacgacg
     2101 gcatcgagcg aactcgcctc gaagggcagc ggctcccagt tcttctcccg ccactccttc
     2161 aacccctgaa agagtccgca atcgacgagc acccggctct tgccgtgctc aatgaggtac
     2221 ttcgaccccg tcaccgtccc cacaccgccc caaaacgaaa gcgtcgccat agctcactcc
     2281 atatcggaag agattgattg actcggcact tgcgcgtgcc tcatcgctat ctcaccgcag
     2341 aggcgcagag gacgcagagt tcacgcagag ggttgacgct tacactcatc gttgagtgaa
     2401 tgcttcaacc ctctgcgctt cctctgcgcc ctctgcgcct ctgcggtgaa ccgaggctct
     2461 caaaactcac cttcgcgcaa tccacaccgc gcgacctcat gactacccgg ccagcgcgac
     2521 gaccttctcg gccgcgtcct tcatgccttg cgcgacagta aaattcatcc ccgattcgcg
     2581 gatgacgcgc tgaccctctt cgacgttcgt gccttcgagg cggacgacga cggggacttt
     2641 gacgccgaga ttcttcgccg cctcgaccac gccgttcgcc accatgtcgc agcggacgat
     2701 gccgccgaag atattgatga ggacggcgcg gacgtgctcg tcggcgagca ggatgcggaa
     2761 ggcggcctcg acgcgctcct gcgaagcgcc gccgcccacg tcgaggaagt tcgccggctc
     2821 gccgcccgcg agcttgatga tgtccatcgt cgccatcgcc agccccgcgc cgttgaccat
     2881 gcaggcgatg ttgccgtcga gcttgatata gttgaggtca aacttcgacg cctcgatctc
     2941 cagcggctcc tcttcgtgcg tgtcgcgcag gtcgcggaag tcggggtgtc ggtagagcgc
     3001 gttgtcgtcg aagttcacct tggcgtcgag ggcgatgagg cgtccgtcct tcgtcagcag
     3061 gaatgggttg atctccatga gcgaggcgtc catctgctcg taagccgagt agagcgtctg
     3121 catgaacttg acggcctgat tcgtgagcga cgcgggcagc cccagcccga aggcgagctt
     3181 gcgcgcctga aaggagcgga agccgacggc cgggtcaatc gtctccttga gaatcttctc
     3241 gggcgagtgc tcggcgacct cctcgatgtc catgccgccg gcggacgagg ccatgaagac
     3301 acttcttcca ctcgcgcggt cgagcacgat gccgagatag aactctttat caatcggcag
     3361 cccctcttcg atgaggacga cgcgcacctc gcgcccctcg gggccggtct ggtgcgtgac
     3421 gaggttcatg ccgagtatct ggctcgccac atcgcgcgcc tcttcgggcg agcgtgcgag
     3481 tttgacgccg ccgcccttgc cgcgcccgcc cgcgtggatc tgcgccttga cgacgacgac
     3541 ctcggcgccg agttccttcg ccgcctcaaa cgcctcctcg ggcgtgcgcg cgacgatccc
     3601 gcgcggcacc gccacgccgt acttccccaa aagctccttg ccctgatact cgtgtatctt
     3661 cattctccgg cccccgctgg atgcggccga cacactccgg ccgcacaagt tttttagaga
     3721 ttgttaggaa cgaacgagag tgtatccgct ggcgcgccgc cgccgcaagc ctcggaagac
     3781 agtgagcagt gagcagtaag cagagggtaa taggtgtttg tgttcaaccg cgcgacgccg
     3841 gttgagtgct tactgctcac tgctcactgc ttactgctca catttctgga atcaccgccc
     3901 gattaacctt ctgttattgg aaacagcgag gacggaggat ttgagcgaga tgacgctggc
     3961 gctgacgctt ctgtttgcca tgaacttgta cgcgcgcgtc gcgaagacga aagcatgatt
     4021 cgccatcttg gggcgaatcg ttaatggcgg gccggctgct cctccggccc gcctcttttt
     4081 ttgccccgcg ccccaccgac cctcccggct cctcggtttg ccacgacgcg gtaatccaag
     4141 ttaattttgc tctcgcgcgg cgacggtgca tgttcaacct aacggggccg cgacgtgcca
     4201 gacgacgccg gaggtgccag ccgatgagtg aagtgatagt cacttccctg tccaacttgc
     4261 agaacgaagt ccgctacggc gatgctcgca cgctcatcac ggatgagccg gcgagcgtgg
     4321 gcggcaacga cgccggcccc gacccgtaca cgctcctgct cggcgcgctc ggcagttgca
     4381 tctcgatgac cgtgtcgatg tacgcgcgcc gcaagggttg gccgctggaa cgcgtcgtcg
     4441 tgcgtctgcg ccagcagcgc atccacgcga gggattgcgc ggagtgcgaa cggacacagg
     4501 agggcttcgt ccaccgcatc gagcgcgccg tcacggtcga gggcgacctg acggacgagc
     4561 agcgcgcgcg ccttctggag atcgcgcaca agtgccccgt ccacaagacg cttgtctcgg
     4621 agatcgtcgt cgcggagttg agcgactgaa cgcttcggcc gcgcccgccg gcaaactttt
     4681 ttccaccccg ccgtcttcgg agatgatcag gacagatgag aagagccttc gctgccttga
     4741 cgcttgccgc cgctttcgcc ttcgttcaac tcgcctgcca gagcgctaag aatcagaacc
     4801 agcccgacaa cacgaacgcg gcgcgcgccg aggccacgcc ggtcgccacg cccgcggccg
     4861 agcacccgct acgccagcag ctcgaagaag aggcgcgaca aagtcccgac gacccgaact
     4921 cgcacttcaa tctgggcaac gtctactttc aggagaggag gttcgaggag gccatcgccg
     4981 agtatcagaa ggtgatcctc gccaacccga acgacgccga gacacatggc cagatcggca
     5041 acgcgcactt cgcgctgaag cgttacagcg aggcagtcga gtcttacaag cgtgccgcga
     5101 gcgcgaaccc gaagaacgcg gagtttcata atctgttggg aaacgcttac gtcgaactcc
     5161 gacaacaccc ggaagccgcc gaggctttcc gtgaggcggc gcgcctcaac ccgaaacacc
     5221 ccgacgcgtt cttcaatctc gccaacgcct acgacgctct cgggatgtac aaggaggagg
     5281 tggcggcgct ccagcaggcc gtgaaagtga acgccaaaga cgccgacgcc tacttcaacc
     5341 tcgggaacgc ctactccaag ttgaatcgcc cgaaggaggc cgccggagcc taccagcggg
     5401 ccgtgaccct caaccccggc gatgcggtcg cgcgcctcac gctcgcccgc acgtacctca
     5461 agaccggcga ccgcaagggc gcggagacgc agcacgaggc gctcaagtca atcgacccga
     5521 actcggccga acaactcctg cgcgagataa atgttgtcca gccatagggc gcgaagtgag
     5581 accgtcaacc agcacctcgc gggcgacaag cgacgtttat tctgacgctc gttttacagg
     5641 aagataattg acaaacggaa cggttggcgt tatcaatacg accgccgacc tcgcggcgtg
     5701 cgtaaactgt tttcgacttc ggttgaccgc acgcgaacca acgctccgac gaccccgtcg
     5761 cccggtctgt gatacaccca ctcaaacgtt ggaaaggttt tcgcccccaa tgaaaaacct
     5821 ccccctgaag atcgtcgcct gtctggcgct ggccgtggcg tgtgtcctgt catcatcttt
     5881 cgtctccgac aatgtctcgg cgcaggtgaa gcaggccaag ccgggcgacc ggctctcgcc
     5941 gctggagaga gagctactca ccgagatgaa tctcgcacgc acgcagcccg ccgagtacgc
     6001 cgcctacctc gaacagctca agcctttttt caagggcaac gtctacacgc cggcgggcgg
     6061 acgctcattc aacacgcagg agggctgggc ggccgtcgag gaagcgatac gcttcctgcg
     6121 cgcggcgaag ccgattgccc cgttcaatgt cgccgagggc atgtgctacg gcgcgggcga
     6181 actcgtcaag gatcagacca agagcggcgg caccggtcac aagggttccg acggcagctt
     6241 ctgcgagcag cgtttgatga gatacggcac gtgggcggcg ccggtcggcg agaatctctc
     6301 ttacggccgc cagtcggcgc gcgaacgcgt gctggagctg atcatcgacg acggcttcag
     6361 cagccgcggc caccgcctgc gaatcttcaa cgccggccac aaggtcgcgg gcatctcctg
     6421 cggcccgcac acctcgctcg gcaacgtgtg cgtgatcacg atggcgggcg gcttcacggc
     6481 ccgggggccg gctcaaaagg ccggcgcgtc gaagccgggc gcgtcgaaga cgggcgaagc
     6541 accggcgccg agcggcgcgg tcagattcta aacgggcctc gccctcgcga ccggcccgca
     6601 cgcggaaatc gaaaagtccc ccggacgttc gtcacgtccg ggggactttt gttatggaac
     6661 aggagaagtc gccgaatgaa cttttgacgt tgacgagtcg ttatgcttct taataatata
     6721 tagctatgtc atatataggc tcacaaatgt tcgaccgcga gttgaagaaa ggcagcgccg
     6781 agttgttgat tctctcgctg gtcgaagacc agccccgtca tggctatgac atcggcagtc
     6841 tgatcgagca gcgttcgcgc ggcgtgctgc gtttcaacgt cgcctcgctc tacccgctac
     6901 tgtaccggct ggagaagcgc ggctggattc tgggtcgctg ggtggagaag gccggtcaaa
     6961 ggcggcggcg ttattaccgc ctgactcccg agggcaaaaa gattctggcg gcgcagcgca
     7021 tcggctggcg ggagtttgtc gaggcgatca atcgcattat aggagtcgaa aatgcctgac
     7081 tggaaagaag aggtcagaag gcgtctgtcg gggctgaggc tggcgccgac gcgcgaggcc
     7141 gagatcgtcg aggagctggc cctgcatctt gatgatcgct acgagcagtt gctacagggc
     7201 ggcgcgacaa aagaggaagc ttacaagtcg gcactgctcg atttgaccga gaacgatttg
     7261 ctggttcagg aactgcgccg ggtcgagcgc cccgcgccgc aggagcctgt ggcgccgggg
     7321 gcgcgaagga ggacgaactt gttaggagag ttctggcagg acttacgtta cggtctgcgg
     7381 atgctgttga aaaatcccgg cttcaccatc gtcgccgtca tcgctctggc tttaggcatc
     7441 ggcgcgaaca gcgcgatctt tagtgtggtc aacacagtgc tgctgcgccc cctgccttat
     7501 aaagaccccg aacggctcgt gatggtctgg gaggacagaa ccaagcgtgg atatccgcgc
     7561 gacacgcccg cggccggcaa ctacactgac tggcgagatc agaatcaggt ctttgaaggc
     7621 atggcggcga ttgccgacct gagttttaac ctgacgggcg tcggcgagcc ggaaagactc
     7681 gacggccggc gcgtctcggc cagcctcttt cccctgctgg gcgtcgagcc gcaactgggg
     7741 cgcgcgttcc tccccgaaga agatcagccc ggcggcaatc gcgtcgttat catgagccac
     7801 ggtctgtggc aacgccgatt cggctctgac atgtcgatca tcggaaagcc tctcaacttg
     7861 aacggcgaga gccacacggt cgtcggcgtg atgccgcccg acttccagtt tccgagccgt
     7921 gacgacgagt tgtgggcgcc catcgctttc gaccctcagg aagccactaa ccgcagaaga
     7981 cactacctgc aaatcgtcgc gcgcacgaag cccggtgtga cgccgcaaca ggcgcaggtg
     8041 gagctgagcg ccatcgccga acgtcttcaa cagcagtatc cggagaccaa tacagacgtg
     8101 ggagccgccg tcgtgccgct gcacgaacag cttgtcggcg acatcaaacc ggccctgctc
     8161 gtgttgttgg gcgcggtcgg atttgtcctc ttagttgcct gtgcgaacgt cgccaacctg
     8221 ctgctcgcgc gcgcctccgt gcgacaaaaa gagacatcca tccgcgtggc gctcggcgcg
     8281 agccgctcgc gtttgatccg acaatttctg acggaaagcg ttttgctggc cgcactcggc
     8341 ggtgtcgccg ggctgctgct gtctctgtgg ggggtgaacc tgctgaaagc tttcatcccg
     8401 gagaacattt ctcaagccca gtccgtcacg atagatggca gcgtgctggc gttcacgctg
     8461 ctggtctcgt tgctgacggg actggtcttc ggtctggccc cggccatgca aacatcgagc
     8521 ttcaatctta acgaagcatt gaaggaaggc ggccgcgact ccgtggcggg aagtcgtggc
     8581 aatcgtcttc gcggcctgct ggtcatcacc gaggtcgcgc tcgcgctcat ccttctgatc
     8641 ggcgccgggt tgttgattaa cagcttcctg cgcttgcgta atgtcgatcc gggatttcgc
     8701 gcggacaatc tgttgacgat gagcatcgtg ctcccgcaac tgaagtatcc cgaccagacg
     8761 cggcgctcgg ctttttataa cgagctggtg agccgtgtgg aatcgctgcc gggggttaaa
     8821 tcggcggccg tcaccaactg gataccttta gtccttcagg gcgattcaat cggcatcagc
     8881 gtcgagggac gccccgaccc cggccccggc caaaggccca gcgcggtgac gagagtcgtc
     8941 agccccgact actttcgcac gatgaacatc cagttgttgc aggggcgaca attcgacgcg
     9001 caggacaaag tcgattctcc atcggtggcg gtcatcagcg aaacgatggc ccgccgcctc
     9061 tggcccggtg aagaccccac gggcaagcgc atcacgcccg gagcgcccga ctccgccaac
     9121 cctgacgact ggataacggt tatcggagtg gtcaaggatg tccggcagtt cgagcttgtc
     9181 gccgagccga agccgcagat gtacctgacg cacatccagg ccggattctt cgcgccgcga
     9241 cacctcgtcg tcagtacgga tgtcgagccg cttagtttgg cgggggccgt gcgtaagacg
     9301 gtgtgggaca tcgataaaga ccagccggtc tccaacgtca atacgatgga atccgtgctc
     9361 tccgaatcaa tcgcgcggca gcgtttcagt atgctgttgc tcggcatctt cgccgccgtg
     9421 gcgctggtgc tggcggcggt gggaatctac ggcgtgatga gctactcggt cgcgcaacgc
     9481 acccacgaga tcggcatccg catggcgctc ggcgcacagc gcggcgacgt gctcaagctg
     9541 gcggtcgggg gagggttgaa actcgtcttg attggtgtcg gggtcggcct cgtcacggcc
     9601 ttcatcctaa cgcgcgtcat gtcgagcctg ttgttcgggg tcagcgccac tgacccgacg
     9661 acattcatca tcatctcgct ggtgctcatc agcgcggccg tgctggcgag ctacatccct
     9721 gcgcgccgcg ccacgaaggt tgacccgatg gtcgccctgc gatacgagtg agtcagtcgg
     9781 ctttctcgac ggagtaatac ttcagaaacg tagggtcagc gccgatggcc ttcactatga
     9841 agcccggctg atcccctctc gcggggactt cctgcgtgta ggcgcggctc gccggagtgt
     9901 tctcctcgcg ggcgcggccc gtgtcaacga catccgcata gcggacgcgg cggccctgct
     9961 cgttcgccac cgccgtgacg tagcgcaccg aagtgtactc cttgttatac gcgaccatga
    10021 ggtgcgtgta gccggggtcg ttcgtgagcc gccacacttc ctgttgcctc ctctcttcct
    10081 tctccaactg accgatgctt tgcaggcgcg cgtgcgcctc ctcacggctc atcccgagcc
    10141 ggatgccgag tatctccggc ttcggctcga ccgatgtggc gcacgccggg gcgagcagca
    10201 gcaggcagca aatggcgacg gcaattttca tcatctttaa ctccgctcgc ggtgtcggcc
    10261 gagtctatca aacattcttc ggccgagtca gccgggccga cgccggcgca taacatgagc
    10321 cggcgggcga cactttcgag aagtcgcccg ccggctcgtc aatgagtcta ccgctatcgt
    10381 ccgcgtcggc gagttaatcg aaggcggcga acagcggctt gccgcccgac tggcggatga
    10441 tcgtcgcgcg atcctcgccg tcgttgtcgt tgccgtcgag ttggccgtcg ccgttcttgt
    10501 cagcgccgct gatacaagcg ggcgtcacgc agccgtcggc gtaggccgcg agcgcacgcc
    10561 cctgtttatc gacggtcacg tcgttgaagt cgagcaggtt gcgcgtggag ccggggcagg
    10621 tcgtgcccga ggtacagacc acgccgcgct gcacggggtc gttcggcgtg gcgttgaccg
    10681 tcacccacgt ctgcccgccg tcataggtcg tggcgatgta gccgaaccat gtgccgggga
    10741 aggtcatgtc tgtcccgcgg ccgttcgcgc ccgatgtggt cgagccgagg aagaagaccg
    10801 cggctcgatt cgcgtcgccg ccgaccgcgg ccgggaagac cgtgttctgg atgccgtgag
    10861 cggcgcccac gtcggagacg ttcacccagt tgagtccgcg gttgtgtgag acggcgactc
    10921 gcgagtggcc gtcgccatcg gcgtaagcga gatagacggt tccgtccgcg ccgatgccga
    10981 ccgacgggtc ggtgtcgccg ggggtgctgc ccggcacggt gcggatgctc cacgtcaggc
    11041 cgttgtcctc ggagacggcg actccctgcg tgccgccgca attcttgttc gggacgtaga
    11101 cggtgccgtc gggcgcgacc ttgatgtgac cgtggaggcc gccgcactgt acgagcgtcc
    11161 acatcgggac ggccacgccg aaggtcagcc cgccgttgtc gctgcgggcg atctgcgcga
    11221 ggccgatgtc ctgcgaggcg tagtagacgg cgttcgggta aaacgcggcg ttgagtccgg
    11281 gcagttggac ggccccgccc ttgaggctgc cgtcggcgtt cttagcataa ggcccgccgc
    11341 cgagggtttg gtggtcaaca cccgagttga tgcctgaacc ctgactcggc gtccacgtcg
    11401 cgccgtcgtc gtcggtgaac gccatcaggc tgagcttccc cgcgagctgc gagacgaagg
    11461 tgcggttggt gcgggaagcg cccgcatcgc tgtcagtaaa tagaattggg tcgagcgtag
    11521 tgatcgaggt ggtcggcgcg ctcacgtcgg cccacgtcgc ggtcgcgggc gacgcggagt
    11581 cgttgaactg gacgcgcagg gtctcaaggc ccgagatgaa catgaccgcg ccagtattcc
    11641 agttcgcgcc gagcgtcggc tcaccggcgc tctcgccgat gccggcgggc gaggcgtagt
    11701 tgcgataggt cggcggcgtt gcgttcgagg gcgtgggcgc gggcgtgggc ggcacgaacg
    11761 gcttcgcctc gacggcggcg cttccgcgat actggtcgag agtggtggcc gcgtagtaga
    11821 cggcgcgcac ggtgtagacg cccgtgcccg acgcggacgg gtcaatcgtc gtgtcttcaa
    11881 tcgtcaacgg ctcggtcggc gacgtgaggc cgcgcccgga gctggcgacg agggggccgg
    11941 cgttcgagcc tttgtggatg tagaggtcgt agtcggtcac ggggagattc cactcgatct
    12001 tgacgcgcac gagcttgccc gcccagtccg cggccgtgcc gccgacgttc aaggtgaggg
    12061 tgtcgcaggt gagtccctca cggcacaaag cctcactgcc gatgacaggc gcggcgttca
    12121 atgcgccgcc gccgatggcc gtccccgtcc aggtgacggg cgcggcggcc gtcgggctga
    12181 gtgttctgga gacggggctg gcggcgtcgg ccgtcatcat ttgcagcggg gcgaaaacca
    12241 ttgtcaaaac aaagatcgcg cccagtgtcg cgccgagcgc cttgaaagca ctctgtgatt
    12301 tcagagttct ggattttctc atcaccaatg tttcctttga cggcggtgtg gggttttcga
    12361 cagggttgcg ccggacaacc ttgccagttc ttcaatagtt caatggcgga cgatgcaatg
    12421 tagcggcggg tgcttggcta attcttggct ggcgcttgag ccttagtgag cggggagttc
    12481 ggggaaagca cttcggattc gcgcccggat ggggggcatg aacacaggga agattcggct
    12541 tgactggcga agtacttgat ggggttgtct ttgtggcagt tatcagttga gtgggggcga
    12601 ggtagattca ccgcagaggc gcagaggacg cggagggggc gctgaggaaa aaatttcgcg
    12661 cccgacacct cgcaagatgc cgggcgcggt ttcaatgcgc gaaagagccg acctttactc
    12721 tgacgcccgt ctggcgatga cggccatcgc gtgggcgagt tcgattctcg cgagcgcgct
    12781 ctgcgggcgg aaggctccgc cctccgcgtt gagcagaccg cgctccagcg cgacggccac
    12841 gtaaccgcgc aaagcggaag ggatcgcccc ggcgtcggtc acgtttaaga gcgcgccggc
    12901 ccttgactcg gcctcggccc tcagccccga cgcgcggact atggcgacgg ccgccgtgag
    12961 gcggtcaacc atgtcgtcgg gcctgaagga agcgcccggt gtcgcgtcag cgaagagagg
    13021 gccgcccggc gcggcctgca cgctttcgac gaagagcatg gtcgaacggt cgagcacgtc
    13081 ggcgtaagtc ggcgcggccg gcgtgtactg cgggacacgc gcgccgagca cgagggccgt
    13141 cgcgaggtcg gagcgcgtca cgccgaacgc cgggcgaaac gaactgccga gcggccacat
    13201 cacgaaggaa cgaaaactct gatagacctc ttcgcgcgct gactggctca aaccgtccaa
    13261 gtccgtcaga ggcgcgtact ctgcgcgggt caactcaagc acgccggaga actgctgagg
    13321 cgtgcccgcc agaccgagcg tgttcgagac gccgacccgc catctgccag tcgcgggcga
    13381 cttgatgaca acgcgctcac gcttgccggt cagtccgggc aggttgagct ggttcgacga
    13441 cgcgcgccgc acaccattgg ggtcgaacag gttcagcccc agatcgttcg tgctgtagat
    13501 cggcccccac gcgatttgga cggaggcgag gagcgtattc tcgggaacgt tcaggaaggt
    13561 ttcgtaaccc gaaagaagtt gcgtcgtgcc gccgaactgc cgcaaggtgt cgcccacgaa
    13621 gcgcacctga ccgcggtcga gagtggcccg ccacaccccg atgagccgtt cggggaaggc
    13681 ggcttcgagc acggccgcgt gcgcgttgag catcccggcc ccgacctcgt gccggtagtg
    13741 gggcgcgagc ggcgtcgccg tgcgctggag gatgtcgcgt acttgagccg gcgtcagatc
    13801 aggatttgct tcgagcatca gggcgatgac gccggccgcc tgcggcgcgc tgaaactcgt
    13861 ccccgtcgcc gtcgtgtagt acggcgctgc ggacgcgggg aggcgccgag aagatgtgag
    13921 gctggaagcg ccgagcacgc ccacgcccgt gtttcgcagg ccgacgacgt tcacgcccgg
    13981 cgcgaccagc gtcgggtgcg gggcgttgcc gccgaatcgg ccgcgcgccg agaaagccgc
    14041 gaggcgtccc gtgtgatctg tcgcgccgac gctgacaacc cacggcgcgg cggcgtaagg
    14101 gttaagcgtt tgcaagcccg gccccgagtt gcccgccgag aagacgacgt tgatgccgcg
    14161 ttcggtaagc aggcgtgtgg cgacgtttac gggatcgttg gcgtcgtaga cggcgttggc
    14221 cgagaagcta cagttgacga cgcgcacgcc gaggtcagga tgtgtgagca ggtagtcgaa
    14281 gcccgcgagc acgtggaaga gattcaaatc cccggcgctg aggcccacga gcttcgcgcc
    14341 gggggccgcg cccgcgtaag cgccgcccga acgcgcgccg tcgcccgcga tcatgccggc
    14401 gacgaaagtg ccgtggccgt agacctgatc cgtattcggc acgttctcca cgccgacggg
    14461 atacgtaaag ccgacgctca agctctgcgt gtccgccagc ttgacgttct gcgcgacgcg
    14521 accggccaga tcggcgtgcg tgccgtcgag gcccgtgtcg atcacggcga ccgtgacgcc
    14581 gcgcccgttt acgttcagtc ccatgttgtg gcgcgtgagg tcggagtcgc gcgcgacgcg
    14641 ttcgacgccc gccggtgcga gatatgggtc ggacgtggtc tgaaaggtgc ggtttccgta
    14701 gatggagcgc accgcgggga ggcgcgagac gttgacgagc tgctgccgcg tcgccgtgac
    14761 ggcgacgacc ggcagcgaca ggtagcgcgt gccgcccgtg atgccgttga gtcgcaggtc
    14821 agcgaggtcg gcgtcgcccg gctgtcggtg gtagacgacc gccgcgttga tgtggctgtc
    14881 gtcagtggcc cggtcgagca ggagggcaag ctcggggtca aggctctgaa ggccggttcg
    14941 ttgctgaacg tccgtccccg tgacggtaat gccgtccgtc ccggtgacgg taatcccatc
    15001 agtgccggtg acggtgatgc cgtcaattcc gttcagcgtg atgccgtccg tgccggtcgc
    15061 cgtgatgccg tccgcgtccg cgatgtggat gctccccggc gtgatggaga agatgcgacc
    15121 gtccgtgccc gtcgcggtga tgccatccgt cccggtgaca gtgatgccgt cagtgccggt
    15181 gacggtgatg ccgttggcgc gggcgataga cagggcgtcg gcgccgatgg cggtgatgcc
    15241 gtcggtgccg gtgacggtaa tcccatcggc gccggtgacg gtaatgcctt cggccttcct
    15301 gagcaggatg gagtcgaggc gggagctgga gccgtcggtt cccgtgacgg tgatgccatc
    15361 cgtgccggtg acggtgatgc catcggccgc ggctacgctc agcgcgtcca cgcctcgggc
    15421 gacgagcgcg ttggccccgg tgtaggtaac gccgtcagtg cccgtgacgg tgatgccgtc
    15481 cgttcccgta acggtgatgc cgtcggcgcc cgtcgtggtg atgccgttga ccttgtaggc
    15541 gaggaggtcg tcagtccccg tgaccgtgat gccggacgtg tcgatgaagg agacgccctg
    15601 cgtgccggtg aggaagacgc cttcgccgcg cctgacgctg acgcccgcaa tcaccgcgcc
    15661 ggcgcaaagc gcgtagacga ggatcacggc gacgatcttt cgcggccttt tcaatccggt
    15721 gctcagcatg gtcacggcga ggtagtgata tttctacaaa ccaaaaaatt tatgcgtgag
    15781 atgaactaca gtgtcggcga cgcccacaac gctcacccga ctctgagtgg ctcggctcaa
    15841 gtggccgcgc ttcccttcag gccgacgaat ccacttcgat aataccgcaa ggctgcgggc
    15901 cgggtaaacg ccgtcggggc cgcacgcccg acggttcact tgattgactc gcggtaaact
    15961 ccctgcgtct cctgagccgg ctcgacgccc agctcattga gcagaaggct gcgcagggtt
    16021 tcgtactgct ccctcacggc gccgcgactg cccagcgccg cgtgcgcgcg catcacgcgg
    16081 caatgaacct cctcgcggaa agggtcgtcg cgcagtatct tctgggcgag cgacaggcaa
    16141 tgttcccact cccccgcctc ctgcgcggcg gaggcgagct tctccagcat gcggagatac
    16201 tggtcgcgat aataggaacg ttgttcctcg acccactcgt cgtgggtgcc ctgcatgaac
    16261 tcgccgcgat agagcgccac cgcctcctcg tatgcctgcc cgcaccgctc cgtctgacca
    16321 cgacggcgcg ccgccgcggc ctcggccacg aggccgtcaa actcctccac gtccgagcgg
    16381 taggagaagt cggggttgag caggtaatcc ccgtctcgat aaagcaaaaa gttctgccgg
    16441 atgagctggt tgctgttgag cgccttgcgg atatacgaga tcgtggggtg aaacttcttc
    16501 tcgacgcttt ccagatcgtc gtcgcgccag aacgtgtcga tcagcgtctc cttcgaggcg
    16561 cgacggtgtt ggcgcgaggc gatgaagcag aggatgtcgc gcgcgcgctt gctcgtccac
    16621 gcgtcggcgg cgagggggtg cgacgggtcg cggtagattt ctaccagacc cagcatgttg
    16681 atcgtcagat cgctcgcctg tcgggccggg gccgtctgaa cggcgagcac cgcggccgca
    16741 tgaggtagtt gtggccgggc ttgaacggat gagagattct gacgcaagtc tctcggcagc
    16801 agcttgagcg cctgttcgga ggagaacaat tgcgggttct gttccacctc gcgccgcagc
    16861 cagtattcgt agtcgtaacg cgcggccaat tcgagcgcgc gccgcaggtg cattaagtaa
    16921 tccccctctc tctctccggc gcgggcgcag gaagcgagcg cgagcgatgc ttgcgcttcg
    16981 tagtagtaaa gattgtgctc gcggaaataa ctgagggccg ggccgagttc ggcctgcgcg
    17041 gcggcgtgct ccccctgcgc gagcatgacg cggcctcggg ccagcaccgc tgtcttcaag
    17101 gccatctcgt ctttgagcgt ggtgcgcgcc tcgacaagat gatccatggc cgaacgggcc
    17161 gccgccgggt cgccctcctg caaggacagc atcgcctgct cttcaagcaa ctcccgccgc
    17221 gcgagatcga ttcccgcctc tttatagatg cgcgccgccc tcgcgtaaaa ctccaacgcg
    17281 cgcggcactt cccgacgctc gcgatagagg ttgccgtagg cctcgaagac ttcgccttgc
    17341 agcacgctca agttgaagag ttgtgagaat tcgagcgcgc gattcagatg agcctcgcac
    17401 tgcgcgacct ctccgcggta gaggtagcag cgcgccatgt tgagatgaac ggccgcctcc
    17461 tgcggaacgg gaggcgagtt cccgccgtat tgaagcgttc gatgcagcca gcgcaacgac
    17521 tcgtcgaagt cgccgcggat caaagccggc agtccgaggt tgtgcgtcat caggcgggcg
    17581 tagtgctcgt cgccgtggtc ttcggcgctc tgcaacgcgg cgcggaattc acgctcggcc
    17641 gccgcccact cgcccttcgc catcagacac agcccgcgcg tgccggcaca cttggcgcgc
    17701 acgaacgatt cttcgtccgc cagctcaacg gcccggtcga ggtaagcgag ggccgtctcg
    17761 atatcgccgt aacgccgcgc gatggtggcc agcgagcgca acgcctcggc ctctccctcg
    17821 cggtcgttat gagcgcgcag gagcgacgcg gcgcgtgtgt gtagcgaacg cgcggagtcc
    17881 agttcgtcgc gcaggcgcgc gacctcggcg cggtagaaga gtgcgcgcgg gtgcgcttta
    17941 gtagcatcgg gcgggagggc atcgacaaag gagacgagtg aggcgagcgc gcccgcggcg
    18001 acccagtgac cgccgcgctc ggcaatgact gaggccgcgc gctcgaagtc ttccgcggcg
    18061 atcaggtgcc gcatggcctg ctcccatccc ccgcgttcga ggtaacagtc ggcgtagcgc
    18121 gcgtgctcgg cggccactcc cgtctggccc agctcgtggc tcaggcgacg ccgcaggaaa
    18181 ctccgaaaga gcgggtggag gcggtactct tcgccgcggt tgtcgctcgc tacggtgagg
    18241 aagacgttgc ggcgcaccag ctccggcagg acgaggcggc tgttcacctc cgggtagaaa
    18301 agcgcgcaca cgtccatctc cacacggtcg agcagagaga tgcgcagcag gaagcgttgc
    18361 acgtcttcct gctcgtcggc gaagacctct tcggcgaaat aatcgaagat gtcgtgctcc
    18421 gactgtcgca gaatctccgt caggtcgggc gcgcccgttt cactcgcccc ggaagctatc
    18481 gcctcgcgtt gggcgaattg cctgacgagt tgcaacgcag tgatccaccc ctgcgtgcgc
    18541 tcgcgatact cggccaactg cgcgggcgtc aattcgaggt cgaacacctg ccggaacagt
    18601 tccagcgtct cttcgtcggt gaagagcagt tcctgtcggt cgataatcac gagcgagtgt
    18661 tgcgagcgca ggcgcgacaa cgcgagcggc ggcacgtcgc gcgagacgac gatgaagtgc
    18721 aacacgtccg gcaggtatga gatcaggcgg tcgagcacgg catgcaccgc cgtgtcgcgg
    18781 ccgaggtggt gataatcgtc gagcacgacg acgagttgct ggctgacctc ttcgaggact
    18841 tcgttcaaaa gcacgtcaac ggcgcgcgct ggctcgcgcg cgatctcgtc ggccgactgt
    18901 cgcagatagg agagcgtcac gtcgccgaag ccgggcacgg cctgcctgat gccgtgcgcg
    18961 aggtagccga ggaagactag cgggtcggcg tccgtgtggt cgagctgata ccaaacgaac
    19021 tgctgcgcgt gggcgcggac gaaatccgcg accagagtgg ttttacccga gccggcgggg
    19081 gcggcgacga gcgtcaccgg gtgcgagagg ttgctctgga ggcgttcggt caaacgcggg
    19141 cgggagagta aggcgggggc cgggcgcggc gggagcagct tcgttcgcag gaagaacgtg
    19201 ggccgacgcg gcgacttctc ttcctccagc gccggctgcg tgagcgccgg cgcgggtgtg
    19261 ctgtcgctgc gagtcctctg tggcatcggg cttgtcttcg gcggctccca caaaatttag
    19321 gctgagctta tcacggcggc cacgctcgtc tccacggggc cgcgggggag gaggcgatgc
    19381 gcgcgcgtga ggcgcgggcg actgctggag tttttaccgg cccgaggccc gccgtctttc
    19441 gcgacgaggt tgactgtttg aaggatagac acgcgagccg cacgcgactc ggcgcgaaga
    19501 atttctctcg acgtgatgaa ttccggcctc cgccctgctc atccacatcg ccgtcttatc
    19561 cagagcggcg gcgcgtcccc gcgtctgttc aagcgccgcg cgcgcgtcgc ctgctccgag
    19621 gccgtaactg gagaacaagt ccacggcctc ctgcggggtg aaagccaact cggcttcttc
    19681 gatgaggcag agcgtctgct tcgagcgcat gcgccacatg ggcgtcggcg gcgcgctgcg
    19741 tccgatgatg agcatgtgcg cctcgggggg cagaagcggg agcagccgtt ggaagaacgg
    19801 cacgacccac gccgcgtcat aaatcaggtg caggtcgtca atcacgatca gcagcggtgc
    19861 gtcctgacac tcctgcaatt catagaggaa cgcctcggca agtgtgggga tgtcttcaag
    19921 acccggcgac ccgtcgatgt gagacaggac tttctgcccg aagccggggc gctgtcggct
    19981 gacggtttcg acgaggtaac ggaagaagat tcgcagctcg ccgtcggaag cgtcaacctt
    20041 gtaccagacc acacgcggag cgcagcgccg cgcgaaatct gtcgccagca gcgtcttccc
    20101 cgtgcccgcg cgccccgcga tgatggtcga agtgcaggag ccgagtccct cttccaacag
    20161 ccggaggagg cgcggacgcg agacgcgtgg gttttcgtgc gggacagaga ccttgtcgag
    20221 aatgattcgc atacttgcta cttttgaaca atccagccgg aacgagtccc gcggccgaag
    20281 cattcgcccg tcagggcgag acggtcgggc cgtcatttaa aagtttacgc atgaaacttg
    20341 ggtgattctt gagtgcctct tcaaaggggg acagtggtgc ggagttggga atgtgcaacg
    20401 gcacgaaaag gacgaaactc agatgttgtg tcggtcgtta agtgtctcga ccctcccaca
    20461 ggctatgccg ctggaaggtg cagtgagagt cttgagcacc acggaagcgg cttccgacgg
    20521 cctgatactc ccttaactat ctcggcccgc agcagcggtg gccgcttacc aacgcagcgc
    20581 ggctcgccgc gcaaaccgat accctcgtat gcttaccatc aacaacctct ccaagaccta
    20641 ccccaacggg gtgcaagcgc tcaagggcgt cagcctcaac atcgagcggg ggctgttcgg
    20701 gctgctcggg cctaacggcg ccggcaagtc cacgctgatg cgcacgctgg ctaccctaca
    20761 ggagccggat accggccaga tcatcttcga cgggctggac gtggtcagcg acccacaggc
    20821 gcaccgccgc caactgggct acctacccca ggacttcggc gtctacccgg gcgtctccgc
    20881 ctacgacctg cttgagcacc tcgcggtcct aaagggcgtt gccgaccgga aggcgcgccg
    20941 cgagcagttg gaggcgctgc tggagcagac caacctgtgg gcgcaccgga agaaggcggt
    21001 gagcggcttc tccggcggga tgcggcagcg cttcggcatc gcgcaggcgc tcctcggcga
    21061 cccacggctg atcatcgtgg acgagcctac cgccggactc gacccggagg agcgcaaccg
    21121 cttccacaat ctgctcagcg aggtgagcga gaacatcgtg gtgattctct ccacgcacat
    21181 cgtcgaggat gtacggcagc tctgcccgcg catggccatc atggccgagg gccgcgtgct
    21241 gcgctcgggc gcgccgcgca agctgatcgc cgagttggag ggccgcgtct ggcgaaagac
    21301 ggtggagaag ccggcggtgg aggcttaccg caagtcgctt cacgttatct ccacccagct
    21361 ctacgaggga cgcacgctgg tgcacgtttt gcacgagggg cggccggaag ctgacttcga
    21421 ggcggtggag ccaaacctgg aggacgtgta cttctccatc ctgcgcgcgc cggcggaggc
    21481 ccgccatgct gcttgaggtg attcgattcg agtggcgcta ccacacccgg cagatcgcct
    21541 ttccggtggg cgtgctcttt tttctgggca tggggttcat cctgccggtg ctgggttacg
    21601 gcccgggcgg cacacacctc aactcgccct tcgtcgtcat gcagtcggtg ggactgctgt
    21661 cgctcctcaa catcttcgtg ctgaccgtct tctgcgcgaa cgcggttgcc cgcgacgccg
    21721 agcacgggat gagggagatc gtgtacgcca cttctatcgg caagctgcgc tacctgagcg
    21781 cgcgcttcgt gggagcgatg gcggccgcgt tgacggtttt ctcctgcacc atcgttggcc
    21841 tgtacctcgc gcccatggtg gccaagattg atccggccaa gctcggcgac cccattccgc
    21901 tggcttacct ttgggcgttg ctggtgatgg cgctccccaa catgctgttc gccggtgcga
    21961 tcatcttcgc gctggccgtg ctcacgcgga gcgtgctggc cagctacgtg ggcagcgtct
    22021 tcctgtacat gctgtacatg gtgatcgcga tgatgatcga ctcgccgctg atggccggat
    22081 cgacgccgca gtcgccgcag gcgatggccc gagcggccat cctcgatccc ttcggcatcg
    22141 ccgccttctt ccagcagaca tggtactgga cgcccgccca gcgagacaca caactgctgt
    22201 cgctcgacgg ctacttcctg atcaaccgcc tgctggtgat gggcgtagcg gcggcaatcc
    22261 tgttggtcac ttaccgctac ttctcgttcc ggctcgccgc cagcgcccgc cccgcgccgg
    22321 ccgaaacgct ggagccacgc gcgacagttg cacgctatcg cggggtgccg gtggccccag
    22381 agtccgcggc gggccgctgg gccgcgctgc gcgtggcgac gctgcgagag ttccgcgcca
    22441 cggccgccag caagcccttc ctcgcactgc tggccctgtg gttatgcgta gcggccatca
    22501 gcgtcgtgga cgtttgggcg gagtaccgta cccagctcta tcccaccacc gggattatgt
    22561 tggaggccgt cgagataccg ctggcgttcc tgacgacgct catgctcgtc tacttcgccg
    22621 cggaggtggt gtggcgcgag cggatcgtcc gcgcggacga gatcgtggac gccacgcccg
    22681 cgccgaacgg ggtcttctat ctggccaagg gcggcgcgct gatcctgctc tcatggctga
    22741 tgacactggt gcccatcctg gtcggggcgg gctaccagtt gagcaccggc tacacgcagt
    22801 tgcagccgga ggtctacctc tcgctgttct acttcacagg attgccgctc gcgctcctgg
    22861 ccgtcgtcgt gctgctggtc cagaccctaa gccccaaccg atacctggga atcatcgtcg
    22921 ggatgatcct gggcctgctg atgctgagtc cgaacgaact gggtctcatg cacgggctgg
    22981 ttcggttcgg cgcccctcca cgggcgccgt acacggagat gaacggattc ctcgcgccgg
    23041 gggtcttcgg cctgtacatg gtgtactggg gcggtttggc gggcgtgctg gcattcatca
    23101 cccttgggct gtggcggcgc ggccgcgcgc agacgctgct gggccgggtg cgggaacttc
    23161 cacgccgctg gggccgccgc ggcttggcgg gtgcggccgg ctgcctggcg gtgttcctga
    23221 gcaccggcgg cttcgtcttc tacaacacca acgtgctcgc ccggtacgag actcgggagc
    23281 agagtctgga ctggcgagca gcatacgagc ggacttaccg aagctacgag cacgtgccgc
    23341 agcccagtgt ggtggcagtg acgaccacgg tagacctcta tccgtcggag cggcgtttcg
    23401 aggtggcggg cgcctaccgg ctggagaacc ggacggcgca gccgttggac acggtgctaa
    23461 tttccctccg gcgcgagctg cggccggagc agatggagct tgcgggtgcg cgtcttgtgc
    23521 ggcacgaccc caccttcggg atgtacgtct tcgcgctccc gcagccgctg gcgccgggcg
    23581 cggccacgga gcttcggttc cgcatcagct cgccgccggt catcgtggag gcgaccggat
    23641 tcagctactc ggtggttgcc aacgggagtt acctgacgcg gcatgccgcg tttccccgcc
    23701 tcgggtacgt gttgggctac gagctggaag acgcgtccga acgccgcgag cgcgggctgg
    23761 ggccgccgcg aaaaggcagg gggctggtga acgacccctc cagcgatgac caccgcgaag
    23821 aatggctgac ggtggatgct accgtctcca cttccgaaga ccagatcgcc atcggcccgg
    23881 gagacttggt gcgcgagtgg cggcaggacg ggcgacgcta cttccagtac cgcacggccc
    23941 agcccgccac gcctcggttc ggtttcgtct cggggcgcta tcaggtgcgg cgggagaacc
    24001 atcgcggcgt ctcggtggag gtctaccacc accctgcgca cggctccaac gtcgagaaga
    24061 tgctggcagt caccacccgg tcgctggaca tgttcggcga gcgcttcggg ccgtacccgc
    24121 accagcacct gcgcatcgtg gagctgccgg ggcactggga cttcggggcg ttcgccctga
    24181 ccgggctgat cgtgtggccg gaagaccgtg gcttccttac tgacgagagc cgcggcggcg
    24241 aggttgacct gatcacccgg cggctggcgc acgaagtgtc gcatcagtgg tggggccacc
    24301 agctctaccc ggcgcaggtg gagggcggca cgatgctagt ggagacgctg gcgaaatact
    24361 cggagctgct ggttctggag gcgacgcagg gacagggtag cctgcctccg atgctgcgct
    24421 acgagcggga gatgtacctg ctgtcgcgga ccaacacgcc ttttccggaa ccgcccctga
    24481 tgcgggtggt ggacttagac cacgtctact actcgaaggg cgccatcgtc atgagcgcga
    24541 tgcgcgacct gatcggggag gatgcgctga accgggcgtt gcggcgactg ctgagcgagc
    24601 acggcgcggc gagccagccc gccaccacgg agaatttgct agccgccttg cgggcggagg
    24661 ccgcgccgga gcatcacgcc ctgatcgatg aatgggttcg cgaggtgtcg ttcct
//
LOCUS       DASVES010000273        10001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_205919, whole genome shotgun sequence.
ACCESSION   DASVES010000273 DASVES010000000
VERSION     DASVES010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205919"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VF240_10985"
     CDS             complement(<1..1472)
                     /locus_tag="VF240_10985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7175774.1"
                     /translation="MKCPDCATPVADARSLSCPSCGASLAESEESFAPTRILEEPPRP
                     SGPSAAGGEDTPTRLGAPPARAPSPSRTPSSRTSRTLSIHDSSIDNSRFVAGTMLDER
                     YRVVGLLGRGGMGEVYRADDLKLEQPVALKLLPEQLSVDGAALSRFHREVRIARQISH
                     RNVCRIYDIGEAEGLHFISMEYVKGEELSSVLKRFGRLPADKALEIARQICAGLAAAH
                     DAGVLHRDLKPANIMIDELGNVRVMDFGLAGLAEEFSGEHAIEGTPEYMSPEQFTGKE
                     LTVKSDIYSLGLVLYELFTGKKAFTANTLPELIRLRRSETMPEHLSMLVRDIDPVVER
                     VIERCMASDPKDRPATALQVAAALPGGDPLQAALAAGETPSPEMVAASSTQGALRPAA
                     AVALFAGALFALALCLYLSPPAMLHRRVPLEKSAIGVRERAGEMARKFGYAEVADTAF
                     GYSIDGEYLEHIRETDKSLTRWDRLASGQPPALLYWYRQSP"
     gene            complement(1697..2206)
                     /locus_tag="VF240_10990"
     CDS             complement(1697..2206)
                     /locus_tag="VF240_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175775.1"
                     /translation="MKRITTVGAAIIAALVLTTGLVVASAKADFSGAWTLDKAKSEGL
                     PPDVKSQTMTVKQTGDSVAVETKTANEGGEEVNAAVYTLDGKEAAHVQKGPNGMEGKG
                     KRISTWAADGNGFDVKETAVFDTPGGVVEVKGTRKWVLAADGKTLTIDMTLETPMGTH
                     TVKRLFTKN"
     gene            complement(2297..2800)
                     /locus_tag="VF240_10995"
     CDS             complement(2297..2800)
                     /locus_tag="VF240_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7175776.1"
                     /translation="MTHANAAESVRIKPATPADVPLILAFVKELAEYERSADKVVADE
                     ESLRESLFGERSVAEGRIAYVGGEPAGMALFFHNFSTWTGLRGLYLEDLYVRPEMRGR
                     GVGRRLLAHLARLARERDCARFEWAVLNWNEPAIGFYQSLGAVPMDEWRIYRLTGDAL
                     DRLAEEA"
     gene            complement(2923..3621)
                     /locus_tag="VF240_11000"
     CDS             complement(2923..3621)
                     /locus_tag="VF240_11000"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX7175777.1"
                     /translation="MTQHIGIVAGSAEGAALCYRTICLEAQGAMGEHNHPEVTLHSLP
                     MAAHMPHVLAGNWERVADLLLQSARKAAEAGADFAICPDNTYHEAFPHLLPRSPLPWL
                     HIAEVVAEEAAGRGFRRLGILGTKYLTEGPVYVEALKKFGVEREIPEGEDRERINTII
                     FKELVNGVFTEESRLYFNEVMERLRARGCDAAVLGCTEIPLLVRPDDAPLPTLDSTRL
                     LARAALRRAIEADN"
     gene            complement(3737..3970)
                     /locus_tag="VF240_11005"
     CDS             complement(3737..3970)
                     /locus_tag="VF240_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175778.1"
                     /translation="MPEVRGKQATEDVKKEWVTAYDFYRRAPGDPYDKKNDRTSRISF
                     VAEKMNLTRKQAKRRVRNYEAWQRNIKKGLVTA"
     gene            complement(4006..5055)
                     /locus_tag="VF240_11010"
     CDS             complement(4006..5055)
                     /locus_tag="VF240_11010"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7175779.1"
                     /translation="MSETFEEISAGDGHELVVLCADTEAGYRSVIALHSTALGPAIGG
                     ARLWRYDSTDAALTDALRLSRGMTYKCALAGLPFGGGKAVILDGGGATQDRVQLFRAH
                     GRFVERLGGRYITAEDVGTSPADMEHVLMETEHVGGLIDRSGDPSPATARGVFRAVQA
                     SARHRWGADDLKGRTVALQGCGHVGYHLARELAEAGASLVVADVSAERAGRVGTEFGA
                     RVVEPREIFDVEADVFAPCALGGVVNDETLPRLRVQVVAGAANNQLLEARHGDELAAR
                     GILYAPDYVCNSGGVINGCRELLGWEPERSRQKIEEIYDTLLAIFRRAEGEGVSTARS
                     ADRLAERRLAQAGTE"
     gene            5265..6248
                     /locus_tag="VF240_11015"
     CDS             5265..6248
                     /locus_tag="VF240_11015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX7175780.1"
                     /translation="MGSETFTPDAGGETFTVVGLGELIWDMLPAGRRLGGAPTNFAYV
                     SRLLGGGADTVAVASRVGRDALGREALERLTRAGVSTEHVQIDATHPTGTVGVTLDAH
                     GEANFNVNQNSAWDYLEWSAGWEELAARADAVCFGTLGQRAAQSRAVIARFLHRTRPG
                     ALRVFDVNLRHAFFDAAMLAASLRLASVVKLGREELPLVAEMTGVGAGDDGDERATAE
                     RLARAFDLRLVAVTRGARGSLLVTPTEAFEHTGFRVRVADTIGAGDAFAAALVNRLLR
                     HATLAEAGDAANRAGAWVTTQSGATPDVDPADVPRILESVGPARPIDDEPR"
     gene            complement(6363..8273)
                     /locus_tag="VF240_11020"
     CDS             complement(6363..8273)
                     /locus_tag="VF240_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175781.1"
                     /translation="MLSPSGDRGSSGKATNAVSENTIKGSKKPSLSAREQKKSTSKAS
                     YESGVEHGLAGRYEEAIKAFQGTIINEPSDADAHFGLGHALFNSGRWDEAAEAFKRVV
                     AINPKDYEAYLYLGASYFRSGQYEQAIDAYKSAVLLKRQSSEARYNIAHAFLRMKLYE
                     PAITYYQDVATFRPKSSEVYNDLGVAYGQSGQYEKAMEALKRAVSLDSGNAYAQSNLA
                     ITYYLLGKYSDAIKAIDRAGRLAPEDFVIQSNHKLAYEKLNGAEIASGEPVSLVNAGG
                     QSTAEGMRWLSRSDVVLGIREKDESAKATEDSAPLVPRAAAPAAAVDGLTLSRSEMPS
                     VTMPEGDAHSAAKPAVAPAKAETPVSNNVPAEETGAAFLVNLYRVGVGDVLDIRLLNE
                     STDRSTLYAVLAGGVLEHPVVDKTFNAVGKTVEEIAAHIKSELHRRALQPDSGVLVNV
                     REYASHVVMISGLVAQPGEKVIKREAIPLYVIVAAAQPLPEAARVTVTSYATGQTTHV
                     ALNDQATMNILVRPGDVIAVSKKLQEFYYVSGDVSNPGQKDFHEGITLTQAIMAAGGK
                     LHKAQNATRAWTRSVLTVGVIAPSANREVSVTRANTDGRLVTTSYDLKDILSGKEPDP
                     LVKAGDRIEVRR"
     gene            9248..9973
                     /locus_tag="VF240_11025"
     CDS             9248..9973
                     /locus_tag="VF240_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7175782.1"
                     /translation="MPLRKLMFCLSAALLMGLATAASARADEVFISGYTNGAFNGVPP
                     NTSATQTATLGGLTYVNSTFSGTTAAGFRALGGNPIAPPVQNVNNLGAFSLATTPFTY
                     TGNTFTLRVTFTAPEGIAGTNTALFSATLTGTVTSDPNSGGVQIDFNNTPMLFTFNDP
                     NCQATTVPGQQTTCGAGSFLFSVNDVSLDPGQIASITGQITSAQQSQPGAVPEPMTMI
                     LFGTGLAGIAAKARRRRKATKAV"
BASE COUNT         1671 a         3239 c         3259 g         1832 t
ORIGIN      
        1 gggctttgcc tgtaccagta gagcagcgcg ggcggctgcc ccgaggcgag acgatcccag
       61 cgcgtcagag acttgtcggt ctccctgatg tgttcgagat actcgccgtc aatcgaatag
      121 ccgaaggccg tgtcggccac ctccgcgtag ccgaatttgc gcgccatctc gccggcgcgc
      181 tcgcgtacac caatcgccga tttttccagc ggcacgcggc ggtgcagcat cgccggcggc
      241 gaaagataga gacagagcgc gagcgcgaag agcgccccgg cgaagagcgc cacggccgcc
      301 gcgggccgga gcgcgccctg cgtcgaggag gccgcgacca tctcgggcga gggcgtctcg
      361 ccggcggcga gggcggcttg aagcgggtcg ccgcccggaa gcgccgccgc cacttgcagg
      421 gccgtggcgg ggcggtcttt cgggtcggag gccatgcagc gttcgatgac gcgctcgact
      481 acggggtcga tgtcgcgcac caacatcgac aggtgctcgg gcatcgtctc ggagcggcgc
      541 aggcggatga gttcgggcag cgtgttggcc gtgaacgcct tcttgcccgt gaagagttcg
      601 tagaggacga ggcccagact gtagatgtcg cttttgaccg tcaactcctt gccggtgaac
      661 tgctcgggcg acatgtactc gggcgtgcct tcgatggcgt gttcgccgct gaactcttca
      721 gcgaggccgg cgagtccgaa gtccatgaca cggacgttgc ccagctcgtc gatcatgatg
      781 ttggcgggct tgaggtcgcg gtgcaggacg cccgcgtcgt gcgcggcggc cagtcccgcg
      841 caaatctgtc gggcaatctc cagagccttg tcggcgggca ggcgtccgaa gcgtttgagg
      901 acggacgaca gctcttcgcc cttcacatac tccatcgaga tgaagtggag cccctcggcc
      961 tcaccgatgt cgtagatgcg gcagacgttg cggtgggaaa tctggcgcgc gattctcacc
     1021 tcgcggtgga agcgcgaaag cgccgcgccg tccacggaga gctgctcggg caggagcttt
     1081 aaggcgacgg gttgttccag cttgaggtcg tcggcgcgat agacctcgcc catcccgccg
     1141 cgcccgagga ggccgacgac gcggtagcgc tcgtcgagca tcgtcccggc gacgaagcgc
     1201 gagttgtcaa tcgatgagtc gtggatggag agggtgcgcg aggtgcgcga agaaggggtg
     1261 cgcgatggcg aaggcgcgcg cgccggcggg gcgccgagac gcgtgggcgt atcttcgccc
     1321 ccggcggccg atgggccgga ggggcgcggc ggctcttcga gtatccgcgt cggcgcgaac
     1381 gactcttcgg actccgcgag cgaggccccg cacgaggggc atgagaggct acgcgcgtcg
     1441 gcgacgggtg tggcgcagtc ggggcacttc atgcgcggca aagtgtaaca cctcggtgtc
     1501 tttcggcggg agcatcatgc ccaagatggc gcgggtgacg gggcaggcgt cgggtgatcg
     1561 gccacagccc tgcctccgaa ggcgtgcgta acccatgccg gaaacgtccg gcagccccac
     1621 gcgaggggcg gcgcacctcg tgagacacgc ggcggcccat cgcgcggggc tgcttctgat
     1681 gcgaccgggt ggcggttcag ttcttcgtaa agagtcgctt cacggtgtgc gtgcccatcg
     1741 gggtctcaag ggtcatgtcg atggtgagcg tcttgccgtc ggccgcgagc acccacttgc
     1801 gcgtcccctt cacttcgacg acgccgccgg gcgtgtcgaa gacggccgtc tctttgacat
     1861 cgaagccgtt gccatcggcc gcccaagtgg agatgcgctt gcccttgccc tccatgccgt
     1921 tcggcccctt ctgcacgtgt gcggcctcct tgccgtcgag cgtgtagacg gccgcgttaa
     1981 cctcctcgcc cccttcgttt gcggtcttgg tctcgaccgc gacgctgtcg cctgtctgct
     2041 tgacagtcat cgtctgactt ttaacgtcgg gcggaagacc ctcactcttc gccttgtcga
     2101 gggtccacgc gccgctgaaa tcggccttcg cggaggcaac gacgaggccg gtcgtcagga
     2161 caagggcggc gatgatggcg gcgcccacgg tggtgatgcg tttcatttga atctcctcgg
     2221 gaataattgg catggcgaaa gcctctgcct tgcggctcgc ggcgtgtgct gctacgggcg
     2281 cccgcgcgaa aaggtttcaa gcctcttcgg ccagccggtc gagcgcgtcg cccgtcaggc
     2341 ggtaaatccg ccactcgtcc atcgggacgg cgccgaggct ttggtagaag ccgatggccg
     2401 gctcgttcca gttgagcacg gcccactcga agcgggcgca gtcgcgctcg cgcgcaaggc
     2461 gtgcgaggtg cgcgagcagc cggcggccca cgccgcgtcc gcgcatctca ggccggacgt
     2521 agaggtcttc gaggtagagg ccgcgcaagc ccgtccacgt cgagaagttg tggaagaaga
     2581 gcgccatccc ggccggctca ccgcccacgt aagcgatgcg tccctcggcg acactcctct
     2641 cgccgaacaa cgactcgcgc agactctcct cgtcggcgac caccttatcg gccgaacgct
     2701 cgtactccgc cagctctttc acaaacgcga ggatgagcgg cacgtccgcc ggcgtcgccg
     2761 gcttgatgcg gacactctcg gccgcgttcg cgtgcgtcat gtttccacct cgaacgatct
     2821 cactgtcatc gaaattaaca tggatggaca agacgggcag gataaggaga tttgaaatct
     2881 gaaatcatct tttcttcatc ctgctcatcc tgtccatcca tgttaattgt ctgcctcgat
     2941 ggcccggcgc aaggccgcgc gtgcgaggag ccgcgttgag tcgagcgtgg gcaggggcgc
     3001 gtcgtcgggg cggacgagga gggggatttc ggtgcagccg aggacggcgg cgtcgcagcc
     3061 gcgcgcgcgc aggcgctcca tcacctcgtt gaagtagagc cgcgactctt cggtgaagac
     3121 cccgttgacc agctccttga agatgatcgt gttgatgcgc tcgcggtctt ccccttcggg
     3181 aatctcgcgc tcgacgccaa atttcttcaa cgcctccaca tagaccgggc cttccgtcag
     3241 atacttcgtg ccaaggatgc cgaggcgacg gaagccgcgc ccggcggcct cttcggcgac
     3301 cacctcggcg atgtgcagcc acggcagagg cgagcgcggg agcaggtgcg ggaaggcttc
     3361 gtggtaagtg ttgtcggggc agatggcgaa atcagcgccg gcctcggccg ctttgcgcgc
     3421 ggactgcaag agcaggtcgg cgacgcgctc ccagttgccg gcgaggacgt gcggcatgtg
     3481 cgcggccatc ggcagcgagt ggagcgtgac ctcggggtga ttgtgctcgc ccatcgcgcc
     3541 ttgcgcttcg agacagatgg tgcggtagca gagggccgcg ccctccgcgc tcccggcgac
     3601 gataccgatg tgctgtgtca ctctcttaat cctttggcgc gtctcgccgc tcgtgcgtgc
     3661 gccgcgcgcg gggggcagaa acccgtgcgc ggcgcaggcg cgggcggtgg tgcgccgcgt
     3721 cagtagccga atgtcttcag gccgtcacca gacctttctt gatgttgcgt tgccacgcct
     3781 cgtaatttct gacgcgccgc ttggcctgct tgcgcgtcag gttcatcttc tccgcgacga
     3841 acgagatgcg cgacgtgcgg tcgttcttct tgtcgtaggg gtcgccgggc gcgcgccggt
     3901 agaaatcata ggccgtcacc cattccttct tcacatcctc ggtcgcctgt ttgcctctta
     3961 cctcgggcat agtctttgta gtctccttaa gtcggggatt aagtttcact ctgtgccggc
     4021 ctgtgcgagt cgccgctcgg cgaggcggtc ggcagagcgc gccgtcgaga cgccctcgcc
     4081 ctcggcgcgg cggaagatgg ctaagagcgt gtcgtaaatc tcttcgatct tctggcgcga
     4141 gcgctcgggc tcccagccga gcagctcgcg gcagccgttg atgacgccgc ccgagttaca
     4201 cacgtagtcg ggcgcgtaga gtataccgcg cgcggccaac tcgtcgccgt ggcgcgcttc
     4261 gagcaactgg ttgttggccg cgccggcgac cacctggacg cgcaatcgcg gcagcgtctc
     4321 atcgttgacg acgccgccga gcgcgcacgg cgcgaacacg tcggcctcca cgtcgaaaat
     4381 ctcgcgcggc tcgacgacgc gcgcgccaaa ctccgtgccg acgcgcccgg cgcgctccgc
     4441 gctcacgtcc gcgacgacga gcgacgcgcc cgcctccgcc agctcgcgcg cgaggtgata
     4501 gccgacgtgc ccgcagccct gcaaggcgac ggtgcggcct ttgagatcgt ccgcgcccca
     4561 gcgatgccgg gccgacgcct ggacggcgcg aaagactccg cgggccgtcg ccggcgaagg
     4621 gtcgcccgag cggtcgatga gtccgccgac gtgctcggtc tccatgagca cgtgctccat
     4681 gtcggcgggg ctggtgccca cgtcctcggc cgtgatgtag cgcccgccca gtcgctcgac
     4741 gaagcggccg tgcgcgcgga aaagttgcac gcgatcctgc gtggcgccgc cgccgtcgag
     4801 gatgacggcc ttgccgccgc cgaagggcag cccggcgagc gcacacttgt aggtcatgcc
     4861 gcgcgaaaga cgcagcgcgt cggtgagcgc ggcgtcggtg ctgtcgtagc gccagagacg
     4921 cgcgccgccg atggcggggc cgagcgccgt gctgtggagg gcgatgacgg agcgatagcc
     4981 ggcctccgtg tcggcgcaca gcacgaccag ctcgtggccg tcgccggcgc taatctcctc
     5041 gaatgtctcg ctcatcggtt gaaaaagtgt ctttaccaaa ggcggggccg ggcgcggaca
     5101 cgcgggcttc cttctcgccg cgtgtaaacg tcgggagcgg aggaaaagtt tttacttcgg
     5161 cggcggcggc cccttcttgc cccgacgcct gcacacttcc cccgtcgttc gtctatcatg
     5221 cttcagactt tccgtagaaa tcaacgggcg aggcgcttcc aaacatgggc agcgagacat
     5281 tcacgccgga cgcgggcggc gaaactttca cggtggtggg cctcggcgag ttgatctggg
     5341 acatgctccc ggcggggcgc aggctcggcg gcgcgccgac caacttcgca tacgtctcgc
     5401 gcctgctcgg cggcggcgca gacacggtgg cggtggcgag ccgcgtcggc cgcgacgcgc
     5461 tcggccgcga ggcgctcgaa cgactcacgc gcgccggcgt ctcgacggag cacgtgcaga
     5521 ttgatgcgac gcacccgacg gggacggtcg gcgtcacgct cgacgcgcac ggcgaggcca
     5581 acttcaacgt caaccagaac tcggcctggg attacctcga atggagcgcc ggctgggaag
     5641 agttggccgc gcgcgccgac gccgtctgct tcggcacact cggccagcgc gccgcgcagt
     5701 cgcgcgccgt aatcgcgcgc ttcctccatc gcacacgacc cggcgcgttg cgcgtcttcg
     5761 acgtaaacct ccgccacgcc ttcttcgacg ccgcgatgct cgccgcctcg ctccgactcg
     5821 cgagcgtcgt caagctcggc cgggaagaac taccgctcgt cgcggagatg acgggcgtgg
     5881 gcgcgggcga cgacggcgac gagcgcgcga cggccgaacg actcgcacgc gccttcgacc
     5941 tccgtctcgt cgccgtcacg cgcggcgcgc gcggcagcct gctcgtcacg cccacggagg
     6001 cgttcgagca caccggcttc cgcgtgcgcg tggctgacac tatcggcgcc ggcgacgcct
     6061 tcgccgccgc gctcgtcaac cgtctcctcc gacacgccac actcgccgag gctggcgacg
     6121 ccgccaaccg cgccggcgcg tgggtgacga cgcaatcggg cgcaacgccc gacgtagacc
     6181 ccgccgacgt gccgcgcatc ctcgaaagcg tcggccccgc ccgcccaatc gatgacgagc
     6241 cgcgatagcg acagtagagg cttgtgccgc cgttacggcg ttaaaaattt aaggcggcga
     6301 cccctcggcc gccgcccctt ctaacaatct cacgtgaggc cgctacccgc gcgccagctt
     6361 gattagcgac gtacctcaat gcgatccccg gccttgacga gcgggtcggg ttccttcccc
     6421 gataggatat ccttcagatc gtaactggtg gtgacaagac ggccgtcggt attcgcccgt
     6481 gtgacgctca cctctctgtt cgcggacggc gcgataacac ccaccgtaag tacggagcgg
     6541 gtccaggctc gcgtcgcatt ctgcgccttg tgcagtttac cacccgccgc catgatcgcc
     6601 tgcgtcagag tgatcccttc atgaaaatcc ttctgtccgg ggttgctcac gtctccactg
     6661 acgtaataga actcttgcag cttctttgag acggcaatca cgtccccggg ccggacgagt
     6721 atgttcatcg ttgcctgatc gttgagtgcc acatgagttg tctggcccgt ggcgtaggac
     6781 gtcaccgtga cgcgagcggc ctcggggagc ggctgcgccg cggccacgat gacgtagaga
     6841 ggaattgcct cgcgcttgat caccttctcg ccgggttggg cgacgagtcc gctgatcatc
     6901 accacgtgac tggcgtactc acgcacgttg accaacacgc ctgagtccgg ttgcagtgca
     6961 cggcgatgca actccgattt tatgtgcgcg gcaatctcct ccaccgtctt cccgacggcg
     7021 ttgaatgttt tatccaccac cggatgttcc agcaccccgc ccgccagcac agcatagagg
     7081 gtggatctat ccgtgctctc atttaacagc cggatgtcta gcacgtcgcc gacgccgacg
     7141 cggtaaaggt tgactaaaaa ggccgcgccc gtctcttcgg ccggtacgtt attagagacg
     7201 ggcgtctctg ctttggctgg cgcaacggcc ggcttggccg cggagtgtgc gtcgccctcc
     7261 ggcatggtca cagagggcat ctcgctgcgc gacagagtca ggccgtctac tgctgctgct
     7321 ggcgcggcag cacgggggac gagtggcgcc gaatcttcgg tcgccttcgc gctttcgtct
     7381 ttttccctga ttcccagcac gacgtcgctc cggctaagcc agcgcatacc ttcggcggta
     7441 ctctggcctc ccgcgttgac aagagatacg ggttcgccgg acgcgatctc cgcaccgttc
     7501 aacttctcgt aagccagctt atgattactc tggatgacga aatcttccgg tgcgaggcgc
     7561 cccgcgcggt caatggcttt gatcgcgtcg ctgtactttc ctaaaagata gtaggtgatt
     7621 gccaggttgc tctgggcata cgcgttgccg gagtcaagac tgaccgcgcg cttcaaggct
     7681 tccatcgcct tctcgtactg acccgactga ccgtaggcga cgcccagatc gttgtaaacc
     7741 tcagatgatt tcggcctaaa ggtcgcgaca tcctgatagt acgtgatggc cggctcgtag
     7801 agcttcatcc tgaggaacgc gtgggctatg ttgtagcgtg cctccgacga ctggcgttta
     7861 aggagcaccg cgcttttgta ggcgtcaata gcctgttcat actggcccga tctaaagtaa
     7921 gacgccccaa ggtagaggta agcttcgtag tctttcgggt ttatcgcgac cacccgcttg
     7981 aacgcttcgg cggcctcatc ccagcggccg gagttgaaga gcgcgtggcc caggccgaag
     8041 tgggcatcgg cgtcggatgg ctcgttgatg atcgttccct ggaaagcttt aatggcctct
     8101 tcgtaacgtc cggccagccc gtgctcgacc cccgactcgt agctcgcctt tgaggtcgat
     8161 tttttttgtt cgcgcgcact aagggagggc ttcttcgagc ccttaatagt gttctcgctg
     8221 acggcgtttg tcgccttgcc agaagaccct ctatcgccgg aaggggaaag caagacccca
     8281 ccacctgacg gagaactcgg tgacgaaccc gtccggtctt gtgcccgcgg agtaaactgt
     8341 agggtacaga ttatgagtag aatgacgagg acgcgcgagg aaaattcttt catcatccac
     8401 ctgactttcg aggcaatggc caacgccaac aaaggaggcg agaccctgga taaacattgg
     8461 gctacgcgaa caggggagag actattgtgt agggcgggaa gtatacaata ttcttctccg
     8521 aaataaaata actgaaagat tgagagatcc aacccttaga cacctaaatc ggcagggact
     8581 tcactaaact gaacgcgcaa ggcggcgggt ctgaacaatt cgccgcgcct gagattgccc
     8641 ggacgaccgg cggaggttag aagccgggat cgtggtgcga ctgtatgcag gtgaagggga
     8701 tgttcaaggg ggcggcccgc ggtgctatag gcgcggccat tggcgccggg ttgaggtgac
     8761 ccggcgcact gccccgctac tacctgggtg tgggcggtgg gaggaactac gccacccttt
     8821 tttgcgttta aatgcctctg tgttgaattt gggcggcgcg ggtcagaccc ggctccaaag
     8881 ctggtaggca gacgcaatcg tggtaacgcc ggacagaatc cacacaacgg gccggtagtt
     8941 tggtatcgct tcgtttcgcg cgctatcgta cacaatctgt tgagaaagtg tggtattcct
     9001 cccacccctc ttgaccagct gccgccgccc atctatactc cacgcctctc ctaacaacgt
     9061 taagtcacac gccgctttgc ccgcgttgat gtgccttctg acgtctctcc cccaatgaga
     9121 aacggctgga gtccggtgcg tttgccccgc cccgtatcag cccatcaact gggtagtcaa
     9181 tgcgactact gctgtcggac gtgtcacgtc aaggttttac agagaacaag acaaggagtc
     9241 taatattatg cccctcagga aactgatgtt ctgcctgtca gcggcgctgc tgatgggttt
     9301 ggccaccgcc gcttcggccc gcgccgacga ggtcttcatc tcgggctaca ccaacggcgc
     9361 cttcaacggc gtcccgccga acacgtcggc gacccagacg gccactttgg gcgggctgac
     9421 ctacgtcaac tcgacgtttt cgggcaccac cgcggccggc ttccgcgccc tgggcggcaa
     9481 ccccatcgcg ccgcccgtcc agaacgtcaa caacctgggc gccttctcgc tggcgacgac
     9541 gccgttcact tacaccggca acacgtttac gctgcgggtg acgtttacgg cgcccgaggg
     9601 gatcgccggc actaacacgg cgctcttctc ggcgactttg acggggacgg tgacgagtga
     9661 cccgaactcg ggcggggtgc agatcgactt caacaacacg ccgatgctgt tcaccttcaa
     9721 cgacccgaac tgccaggcga cgaccgtgcc ggggcagcag acgacgtgcg gggcgggcag
     9781 tttcttattc tcggtcaacg acgtatcgct cgacccgggg cagattgcct cgattacggg
     9841 ccagatcacc agcgcgcagc agagccagcc gggcgccgtg cccgagccga tgacgatgat
     9901 cctcttcggc accgggctgg ccggcatcgc cgccaaggcc cgcaggcgcc gcaaggcaac
     9961 gaaggctgta taagcgccga ctaagtatgg ggcacagacc g
//
LOCUS       DASVES010000274         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_351528, whole genome shotgun sequence.
ACCESSION   DASVES010000274 DASVES010000000
VERSION     DASVES010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351528"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VF240_11030"
     CDS             <1..432
                     /locus_tag="VF240_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7175783.1"
                     /translation="LGYPVFKTRFATIGVYICYDRHFPEGARLLGLNGAEIVFNPSAT
                     VAGLSEYLWKLEQPAHAVANGYFVGAINRVGHEQPWDIGEFYGQSYFCDPRGQIVAEA
                     PRDADHLVVADLDFDKIREVRNTWQFFRDRRPDTYDALVAD"
     gene            451..762
                     /locus_tag="VF240_11035"
     CDS             451..762
                     /locus_tag="VF240_11035"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7175784.1"
                     /translation="MMVKTKEDVLSLLRRSGADLRRFGVERLSVFGSFARGEPNDKSD
                     VDILVEFEAGRTSFDNFLALAEFLEALFGRKVDLLTPESLNPRFGHHILEEAEHATIN
                     A"
     gene            743..1015
                     /locus_tag="VF240_11040"
     CDS             743..1015
                     /locus_tag="VF240_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX7175785.1"
                     /translation="MRPSTREFLEHILDEARFLLDKSRGVDEDSFLRDETLKRAFVRS
                     IEVMGEAVKQIPDSVRQKHPSVEWRAIAGMRDRLIHAYYGVNYRLV"
     gene            1112..2512
                     /locus_tag="VF240_11045"
     CDS             1112..2512
                     /locus_tag="VF240_11045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7175786.1"
                     /translation="MSTDNATSLSHEQIERNFREIAPPLTSAEALFEANRCLYCFDAP
                     CMHACPTHIDVPAFIKKIATGNLTGSARVIFDANPIGATCAHVCPVDVLCEGACVEKT
                     LLAKPIEIGRLQRFATDHVTGRGRQLFKAGEPRGKSVGVVGSGPAGLSCATYLARLGY
                     DVTIYDRRAKPGGLDTYGMAEYKMTQRVSLEESALVAALGVKLKLDTEIGRDLSFDDL
                     EAAHDSVFLAVGLGATQPLGIPGEDLEGVHDALNFIERVKTREWASVPVGRTVAVVGA
                     GNTAVDAATQSKRLGAERVLMVYRRTAREMPAYEYEYELAKQDAVEFLWQTAPVEVLS
                     DGAGRVSALRCVRMELGEPDEAGRRTVSPTPGSEFDVPCDMVIKATGQQKMRELLGRI
                     QGLEFDAAGRVRVDKQTMQTGNPKYFAGGDCVNGGREAVDAAQMGKLAAQGIHLALAG
                     EVVEFAGANTTVNGKS"
     gene            2634..>3227
                     /locus_tag="VF240_11050"
     CDS             2634..>3227
                     /locus_tag="VF240_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX7175787.1"
                     /translation="MADLSVNFAGIKSPNPFWLASAPPTNMGSMVERAFDAGWGGAVW
                     KTLGEPIRNVCSRLGSIDSGTQRMIGLNNIELITDRPLDVNLREIAECKKKYPKHALI
                     VSLMVESNREAWHEIAKRVEGTGADGVELNFGCPHGMSERGMGSAMGQVPEYTCMVTE
                     WVKEATRLPVIVKLTPNVTSINPPGRAAVRGGADALSL"
BASE COUNT          607 a         1046 c         1031 g          543 t
ORIGIN      
        1 ctcggctatc cggtgttcaa gacccggttc gcgacgatcg gcgtctacat ctgctatgac
       61 cgccattttc ccgagggcgc gcggctactc ggcctcaacg gcgccgagat cgtcttcaac
      121 ccgtcggcca ccgtcgcggg cctctccgaa tatttgtgga aactggagca accggcccac
      181 gccgtcgcca acggctactt cgtcggcgcc atcaaccgcg tcggccacga acagccgtgg
      241 gacatcggcg agttctacgg ccagagctac ttctgcgacc cacgcggcca gatcgtcgcc
      301 gaagccccgc gcgacgcaga ccacctcgtc gtcgccgacc tcgacttcga caaaatccgc
      361 gaagtccgca acacctggca gttcttccgc gaccgccggc ccgacaccta cgacgcgctc
      421 gtcgccgact gacttattac cgggcaaagg atgatggtta aaacgaaaga ggatgtcctc
      481 tcactgctgc gccgaagcgg cgcagatctg cgccgcttcg gcgtcgagcg gctcagcgtg
      541 ttcggctcct tcgcccgcgg cgagccgaac gataagagcg acgttgacat cctcgtagaa
      601 ttcgaggcgg ggcgaacttc cttcgacaat ttcctcgcgc tcgccgaatt cctcgaagcg
      661 ctgtttggta gaaaagttga cctgctgacg ccggaatcgc tcaacccgcg tttcgggcat
      721 cacatactcg aagaagccga gcatgcgacc atcaacgcgt gaatttctgg aacacatcct
      781 cgacgaggcg aggttcctgc tggataaaag caggggcgtc gacgaggatt ccttcctccg
      841 tgatgagacc ctgaagaggg ccttcgtgag gagcatcgag gtgatgggcg aggccgtcaa
      901 gcaaatcccg gattcggtgc ggcaaaagca cccgtccgtc gagtggcgcg cgattgccgg
      961 gatgcgggat cgcctgatac acgcttacta tggggtgaac taccgtctcg tctgagacgc
     1021 cgtgaccaac aaagttccgt ctctcgccga aaacattcag ctcatcctga cgggggacga
     1081 catttagact ttaaccgaag cctctaaaag catgtcaaca gataacgcga cctccctatc
     1141 gcacgagcag atcgagcgca acttccgcga gatcgcgccg ccgctgacct cggccgaggc
     1201 gctgtttgag gccaaccgct gcctctactg cttcgacgcg ccgtgcatgc acgcctgccc
     1261 gacgcacatc gacgtgccgg ccttcatcaa gaagatcgcc accggcaacc tgacgggttc
     1321 cgcgcgcgtc atcttcgacg ccaaccccat cggcgccacc tgcgcgcacg tctgccccgt
     1381 ggacgttttg tgcgagggcg cctgcgtcga gaagaccctg ctggcgaagc ccatcgagat
     1441 cggacgcctc cagcgtttcg cgaccgacca cgtgacgggc cggggccgac aactcttcaa
     1501 ggccggcgag ccgaggggca agtcggtcgg cgtcgtcggc tccggccccg cggggctgtc
     1561 gtgcgcgacc tacctcgcgc gcctcggcta cgacgtgacc atctatgaca ggcgcgccaa
     1621 accgggcggc ctcgacacct acggcatggc cgagtacaag atgacgcagc gcgtctcgct
     1681 cgaagagtcc gcactcgtcg ccgcgctcgg cgtcaaactc aaactcgaca ccgagatcgg
     1741 ccgcgacctc tccttcgacg atcttgaggc cgcgcacgac tcggtctttc tcgccgtcgg
     1801 actcggggcc acgcagccgc tcggcatccc cggcgaagac ctcgaaggcg tccacgacgc
     1861 gctcaacttc atcgagcgcg tgaagactcg cgagtgggcg agcgtgcccg tggggcgcac
     1921 ggtcgccgtc gtcggcgcgg gcaacacggc cgttgacgcc gccacgcagt cgaagcgatt
     1981 gggtgcggag cgcgtgctga tggtctaccg acgcaccgcg cgcgagatgc cggcctacga
     2041 gtacgagtac gagcttgcca agcaggacgc cgtcgagttc ctgtggcaga cggcgccggt
     2101 cgaagtgctc tcggacggcg cgggccgcgt gtcggcgctg cgctgcgtcc gcatggagtt
     2161 gggcgagccg gacgaagcgg gaagacgcac ggtcagcccc acgcccggct ccgagttcga
     2221 cgtgccctgc gacatggtca tcaaggcgac gggccagcag aagatgcggg agctgctggg
     2281 ccggattcag ggtctggagt tcgacgcggc gggccgcgtc cgcgtggaca agcagacgat
     2341 gcagacgggc aacccgaagt acttcgcggg cggcgactgc gtcaacggcg ggcgcgaggc
     2401 cgtggacgcc gcgcagatgg gcaagctcgc ggcgcagggc attcacctcg cgctcgcggg
     2461 cgaggtggtc gagttcgcgg gcgcaaatac taccgtgaat ggtaaatcgt aaatcgtgaa
     2521 tagaagttga cgcctatcgt ctttgcgatt tacctctcaa gcttgagcag tctcaatcac
     2581 gatttaccat ttaccattca cgatttacga ttcacgattc acgaggtttt gaaatggcag
     2641 acctgagcgt taatttcgcg ggcatcaaat cgccgaatcc cttctggctg gcgtcggccc
     2701 cgccgacgaa tatggggtcg atggtcgagc gcgctttcga cgccggttgg ggcggcgcgg
     2761 tgtggaagac tctgggcgag ccgatccgca acgtctgctc gcggctcggc tccatcgact
     2821 cgggcacgca gcggatgatc ggcctcaata acatcgagct gattacggat cgcccgctcg
     2881 acgtgaattt gcgcgagatc gcggagtgca agaagaagta tccgaagcac gcgctcatcg
     2941 tctcactgat ggtcgaatcg aaccgcgagg cgtggcacga gatcgccaag agggtcgagg
     3001 gcacgggggc cgacggcgtg gagctgaact tcggctgccc gcacggcatg agcgagcgcg
     3061 gcatggggtc ggcgatgggg caggtgcccg agtacacctg catggtcacg gagtgggtca
     3121 aggaggcgac gcggcttccc gtcatcgtca agctcacgcc gaacgtgacg agcatcaacc
     3181 cgccggggcg cgcggccgtg cgcggcggcg ccgacgcgct ctcgctc
//
LOCUS       DASVES010000275         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_28871, whole genome shotgun sequence.
ACCESSION   DASVES010000275 DASVES010000000
VERSION     DASVES010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28871"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VF240_11055"
     CDS             complement(<1..510)
                     /locus_tag="VF240_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175788.1"
                     /translation="MCLLKHAGGAFTHRPEELKDRLSRRASDLVKKAFEDIEPARLVD
                     YHTHIAGTGAGDTDNLVNPKMLSWKHPLHHLKFAVYLSAAGVENLERADAEAAARLVR
                     LVENIEHHGKYRLLAFDKNYNRDGTVNLEKTEFYVPNEYVFSLAAAHPQLFEPVISVN
                     PYRADAVEEL"
     gene            complement(522..1325)
                     /locus_tag="VF240_11060"
     CDS             complement(522..1325)
                     /locus_tag="VF240_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEX7175789.1"
                     /translation="MPARPAHPTPYADVNAVLHALLAGARKILGDRFAGLYLYGSLAS
                     GDFDPELSDIDFLVVTEGEMPDELLPALEAMHAGLASADRKWATKLEGSYVPRHALRR
                     YDAADPPCPQLNEGRFYVARHCSDWIIQRHVLREHGVALSGPPPSTLIDPVSPDDLRE
                     AVRGILREWWEPMLLDGAWLRRGDLYQAYAVLTMCRALYTLRHGRIASKPVSAAWAQD
                     ELGARWATLIERASAWRHDAPSGDLSGATDFVRFTLGRARSSESSEATV"
     gene            1634..2167
                     /locus_tag="VF240_11065"
     CDS             1634..2167
                     /locus_tag="VF240_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX7175790.1"
                     /translation="MARELSAGAGLERRPRGAAQADVERSLEQLSHWLDGAFRVPGTG
                     WRFGLDALVGLVPGVGDLATTAVSFYILAAGVRYRVSKATLLRMGANLGIDYVLGSVP
                     VLGDLFDVYWKSNQKNVELLKQRATVSPEEARRGRVSDWLFVGVIMLVLLLTLIGAIT
                     VSLYLLSLIFRNVPSPF"
BASE COUNT          418 a          785 c          826 g          443 t
ORIGIN      
        1 aagctcctcg accgcgtccg cgcgataggg gttgacggag atgaccggct cgaacagttg
       61 cgggtgcgcg gcggcgagtg agaagacgta ttcgttgggg acgtagaact ccgtcttctc
      121 cagattcacc gtcccgtcgc ggttgtagtt cttgtcgaac gcaagcaaac gatacttgcc
      181 gtggtgctcg atgttttcga ccagacggac gagacgcgcg gcggcctccg cgtcggcccg
      241 ctcaaggttt tcgaccccgg cggcgctgag gtagacggcg aacttgaggt ggtgaagcgg
      301 gtgcttccac gagagcattt tggggttgac gagattgtcc gtgtcgcccg cgcccgtgcc
      361 cgcgatgtgc gtgtggtaat cgacgagacg cgccggctcg atgtcctcga aagccttctt
      421 gacgaggtcg gaagcgcgcc gactcaagcg gtctttcaac tcttcgggcc ggtgcgtgaa
      481 ggccccgccg gcgtgcttta aaaggcacat gactccacct ctcaaacggt cgcttctgat
      541 gactcggatg aacgggcgcg cccgagcgtg aaccgaacga agtccgtcgc cccgctcagg
      601 tcgccggagg gcgcgtcgtg tcgccacgcc gaggcccgct cgatgagtgt cgcccagcgc
      661 gcgcccaatt catcttgcgc ccacgcggcc gagaccggct tcgaggcgat gcggccgtgt
      721 cgcagtgtgt agagcgcgcg acacatcgtg agcacggcgt aggcttgata caggtcgccg
      781 cgtcgcagcc acgcgccgtc gaggagcatc ggctcccacc actcgcgcag aatcccccgc
      841 acggcctcac gcaagtcgtc cggcgagacg gggtcgatga gtgtggacgg cggcggcccc
      901 gacagtgcga ctccgtgctc ccgaaggacg tggcgctgaa tgatccagtc gctacagtgg
      961 cgcgcgacgt agaagcgccc ctcgttgagt tgcgggcaag gcgggtcggc cgcgtcgtag
     1021 cggcggagcg cgtggcgcgg gacgtaggag ccttccaact tcgtcgccca cttccggtcg
     1081 gccgaagcga ggccggcgtg catcgcttcg agcgccggca gcaactcgtc cggcatctcg
     1141 ccctcggtga cgacgagaaa gtcgatgtcg ctcagctcgg ggtcgaagtc gccactggcg
     1201 agtgagccgt acagatagag gccggcgaag cggtctccta aaatcttgcg cgcgcccgcg
     1261 agcagcgcat ggagcacggc gttcacgtct gcgtatgggg taggatgagc ggggcgggcc
     1321 ggcattcggg tctcaacctt cggcgcgaac ggcttttcag accttgccgt ttcagagtat
     1381 cacagtccgt gcgcgcgttt accgcggctg gcggaacgag cgacgaacct gacgggcgcg
     1441 cgcttaaggg ttgacgacgc cggcaagctc gcggagtttt ggactgacgc gaagacgcgc
     1501 gaaacaaacg cgggcacgcg tcgtattctt tagttcgccg gggcttcctt aaagcctcgc
     1561 cgactcttgg ttcttcggcc ttctcccgcc gacctttcgg tcatccgagt tgaagcgaca
     1621 aggagtgaac atcatggcga gagaattaag tgcgggggcg ggactcgaaa gacggccgcg
     1681 cggggccgcg caggcggacg tggagcggag cctcgaacag ttgagtcact ggctcgacgg
     1741 ggcgttccgc gtgcccggca cgggctggcg cttcggcctc gacgcgctcg tcggattagt
     1801 ccccggcgtc ggcgacctcg cgacgaccgc cgtctccttc tacatcctcg ccgccggcgt
     1861 ccgctaccgt gtgtcgaagg ccacgctcct gcggatgggc gccaacctcg gcatcgacta
     1921 cgtgctcggc tcagttccag tcctcggcga tctcttcgat gtctactgga agtcgaacca
     1981 gaagaacgtc gaactgctca agcagcgcgc gaccgtctcg cccgaagagg cgcgacgcgg
     2041 ccgcgtgagc gactggctct tcgtcggcgt catcatgctc gtgctgctgc tcaccctcat
     2101 cggcgccatt acggtcagcc tctacctgct gagcctcatc ttcaggaacg tgccgtcgcc
     2161 gttttagaac gacctcggca agcacgaggt tgaaaacatc atccgaccag ccgcgccagc
     2221 ggcggcagtc aatggccgca gagcggcgat agttaaatga gccgcgaagc ggcggcagcc
     2281 aatagccccg tccgtaaggg cggggtcaca aggccggtaa taaaagttga gccccgaagg
     2341 ggcggcactc ggtttcacat ccggtgtgcc gccccttcgg ggctcaactg tttttcccgc
     2401 cgcataccac gggcttacgc ccgtggctac tggctgccgc cgcttcgcgg gtggttgaat
     2461 tatcttcgtc cg
//
LOCUS       DASVES010000276        12510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_813529, whole genome shotgun sequence.
ACCESSION   DASVES010000276 DASVES010000000
VERSION     DASVES010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813529"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /gene="selD"
                     /locus_tag="VF240_11070"
     CDS             complement(<1..689)
                     /gene="selD"
                     /locus_tag="VF240_11070"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX7175791.1"
                     /translation="MLAQVLGGLKISADPNLIVGIETADDAGVYRLRDDLALVQTVDF
                     FTPIVDDPFDYGRIAALNSINDVWAMGGVPITALAVTCFPKKGVDFQILGEIMRGGLS
                     VLEENGVALVGGHSVDNAQIMFGYAVTGTIHPERVVTNRGARAGDHLILTKPIGTGVI
                     STGIKFGKAAVETVEASLKTMLLSGRRAAAAMVEAGAHAATDVTGFALLGHAFELARA
                     SSVTLEIDAARV"
     gene            1081..1428
                     /locus_tag="VF240_11075"
     CDS             1081..1428
                     /locus_tag="VF240_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175792.1"
                     /translation="MRDENDFFDDLIDDEAETYTSEASDEDDFEGDDDEDDEEFETRT
                     AVLSKKGMLALLTVKTNEDGGQIVRVDPRQQLPTAQRYDTPESAIEWFRRSLATSRRN
                     GWEVVYDGAPAQG"
     gene            1486..3819
                     /locus_tag="VF240_11080"
     CDS             1486..3819
                     /locus_tag="VF240_11080"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7175793.1"
                     /translation="MPATNNNPETRTGNAATALALTAFVALAAYLSVALIQPPTPAPI
                     DAPPTEFSSARATEHLRAVAREPHPTGSDASVRVREYILSELAALGISAEVQRATAVW
                     ESRRREQPSQAATVHNIVARMAGSAPGAKALMLAAHYDSAPTGPGASDDGAGVAAILE
                     TVRALKTGAPLKQDLIVLFTDGEELGLLGARAFSDEHTWMRDVGLVLNFEARGRGGPS
                     FMFETSEGNEALIAEFARAAPRPVANSLMYALYKRLPNDTDLTVFKAAGAAGLNFAYV
                     SDITHYHTALDAPERADERSLQHHGSYALALARHFGNLDLAPLSGAGRGDAVYFNALG
                     PVFVSYPEAWAALLTAVVALAFAVVVCVGWRRARLSAGGVGLGLALPAVCVLAAVVAG
                     AGGSRLIARLHTDYRALPFGTPYDAWRFEWALVLLVVAVCAAVYAAAGRRAGASSLTI
                     GAMLWWLLLLFASTALLPGGSFLFAWPLLFALVGLSYTFFGRSRATWMESAMLAAGAA
                     PAVVLGAPIVYMLFVLLGAGTSAPALVLAALLASLLLPLLRLSAARGAWLLSAAAGVS
                     AFAFFVAGVTGARFDAERPRINSVFYEMNADTGQARWVSADAAPDAWTSQFFAATARR
                     DVSASVFPWETGTVLTGEARTLDAPPPALQVLEDRADGWLRRVRLRISSQRGAPLMLV
                     YTGAEAEVQSAEVGGKKLAGAGEAGNQLRVFYTGVPVEGLDVRLEVNASRPLKLTVVD
                     GAYGLPETFGIAPRPSGMMPKPSKIGDVTLVRKSFTL"
     gene            4040..5002
                     /locus_tag="VF240_11085"
     CDS             4040..5002
                     /locus_tag="VF240_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEX7175794.1"
                     /translation="MKAIRVHEFGAPEVMRLEDVPEPRAGAGQVLVRVRAAGVNPVDT
                     YVRSGAYAKLPELPYTPGSDAAGIVEMVGEGVTRLRAGDRVYLAGSLTGAYAELALCD
                     ESQAHTLAERVSFAQGAGVYVPYATAYRALFQRALARAGEWVLVHGASGGVGVAAVQL
                     ARAAGLFVIGTGGTEEGRRLAREEGAHHVLDHRAPDYLEQAVALTGGRGVDVILEMLA
                     NVNLDRDLGVLARRGRVVVIGNRGSVEINPRAAMMRDADILGMSLLNTPPEELSGIHA
                     ALVAGLEAGTLRPVVGRELPLAEAARAHAAVLEPGAYGKIVLAP"
     gene            complement(5651..6349)
                     /locus_tag="VF240_11090"
     CDS             complement(5651..6349)
                     /locus_tag="VF240_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7175795.1"
                     /translation="MTQPSRILVVDDEPQIARVLRTGLKTHGYDVRVAADGLSALETF
                     NDWNPDLVVTDLSMPNLDGLELCRRLRQVSQIPIIVLSVRGEEKIKVESLDAGADDYV
                     TKPFGMDELLARVRAQLRRTAALPKSEASPVLEVGDFRVDLEARSVFVRGAEVHLTPK
                     EYELLVHFVRNVGKVLTHHTLLGAVWGGNYTEQGEYLRVFVGQLRKKIEPNPSTPRYI
                     LTEPWVGYRFNSDS"
     gene            complement(6346..7593)
                     /locus_tag="VF240_11095"
     CDS             complement(6346..7593)
                     /locus_tag="VF240_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7175796.1"
                     /translation="MAFGKSRMGYVAAVAGVAAVTTALKLSAGHINAAHINAATVALA
                     LLLVVLFVATGWGARPAVAASLIGVLCFNFFFLPPYGTLTIDAWDNSIALVAFLITAV
                     TVGQLSAHAKRRTEEADAGRQEIERLYTELRDAFERASHAEALRQSERLKSALLDAVT
                     HDIRTPLTSIKASISTLLEELRARTPGRESVALDAEARREMLEVIDEECDRLNRFVEG
                     LIDLARIEAGELQLRRRWGAVDEIITAALTRAEPLTRRHEVRVNIEDEIPVVQVDPRA
                     VSEVIFTLVDNAAKYSPADTTILVAAERADDRAIRIAVENEGESIPRDLHERVFEKFF
                     RATRDGDAGDGRRLKGTGMGLAVAKGIVEAHGGRIWIEDGREGAGTRVSFTLPVGDAE
                     EIPEPSPEVVSETAPSAELNRVS"
     gene            complement(7656..9593)
                     /locus_tag="VF240_11100"
     CDS             complement(7656..9593)
                     /locus_tag="VF240_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX7175797.1"
                     /translation="MAGTASADIHTTLTDAQGHPHTAKPRGRYLAILSLTALGIVYGD
                     IGTSPLYAMRECFHGPHAIAPTPENIFGVLSLIFWALVLIISIKYCVFVLRADNHGEG
                     GILALTALATPIKIVSKSERKWLVVLGIFGAALLYGDGIITPAISVLGAVEGLNVATP
                     LFAPYVVPATVVIIVGLFLIQSRGTAKVGRLFGPVMLAWFFVISALGVAQIVRHPSVI
                     GAFNPLHGFSFFIRNGWHGFLVLGTVFLVVTGGEALYADMGHFGKRPIRIVWYALVLP
                     ALLLNYLGQGALLIENPAVAENPFYRLAPEWALYPLIALATCAAIIASQALISGAYSL
                     TMQAIQLGFMPRLKIEHTSSTEMGQIYIPALNWALMIGCIAIVIGFGSSSNLAAAYGV
                     AVTATMVITTILLGVVTREKWGWGLPLTLGLSGFFLVIDLAFFGANIIKIPAGGWFPL
                     VVGAGIFLFMMTWKKGRRILASRMLSLAIPIEDFLRDIAKTPPVRVPGTAVFMNGTGA
                     GTPPALEHNLEHNKVLHERVLLLTVKTRQVPHVEPEERVSVQALGHEFYRVVINYGFM
                     EDPDVPSVLASMETPGLAFDPAETTYFLGRETVIAGKHPGMMLWREKLFALMSRNASP
                     ATAYFCLPPDRVVEMGSQVEI"
     gene            9961..11655
                     /locus_tag="VF240_11105"
     CDS             9961..11655
                     /locus_tag="VF240_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7175798.1"
                     /translation="MLKVRTFFRKHILWVGLVAVVVPLLSILAVQYNSLKKLEKTSSV
                     ADKMWMKNYLSDVSKEVKFFYHTNAEQVLNVPAYSVSAENLHKVRNHFGKCEVEGSKR
                     LFIVAFDEGGGSQTLFFDPRAQAQLVEATPAEVRAANIVAAPYKMLSQEGTAVAGAKI
                     SEDLRDPQNRVIFKPITDEWARVVGVAGMILDTEFFREKFLPRVIAATLPKFFPDEAR
                     DNVIVTVYDSEQKLVHATQALKGQNDEIATNLPFFYDYRLGIRSRHMTPEQWARWNFN
                     FNLTLSLLMTLVLVSGIVLALRMASREMHLSQMKTDFVSNVSHELRTPLSSIRVFGEF
                     MKLGRVRDPEKMREYGEHIETESRRLTQLINNILDFSKIESGRKTYRVEQVCVEELIA
                     EVLKTCEVRLRQNDFAIVYEPSSQPLPAAFIDRDAIEQALMNLLDNAVKYSESAEVRE
                     VVVRAGYGGGLIRISVTDHGVGIPASEQGKIFEKFYRVSTGLVHDVKGSGLGLSLVKH
                     IVEAHRGRVTVESEPGRGSTFTILLPAAGGAAGQEETPQPTDAIGGEQPLNLGYKH"
     gene            11871..12314
                     /locus_tag="VF240_11110"
     CDS             11871..12314
                     /locus_tag="VF240_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7175799.1"
                     /translation="MKKLMLLAVVTAAAGLAAWLPARAQSAEEAAVRAAIEHYFRAHA
                     TGLGEHHRKVFHPDARLFAVRDGKYWTLTSEEYISRAPGKPPADEAERKRAVEMVDIA
                     GNAAVARVVLDYPDVKFTDYMSLLKIDGEWKIVNKTFHAEPKTKK"
BASE COUNT         2244 a         3989 c         4137 g         2140 t
ORIGIN      
        1 acgcgcgcgg catcaatctc cagcgtgacg gagcttgcgc gcgccagctc gaaggcgtgg
       61 ccgaggaggg cgaagccggt cacgtccgtc gccgcgtgcg cgcccgcctc gaccatcgcg
      121 gccgccgccc ggcgaccgga gagcagcatc gtcttcaagg atgcttcaac cgtctccact
      181 gcggccttgc cgaacttgat gcccgtggag atgacgcccg tgccgattgg cttcgtcaga
      241 atcaagtggt cgcccgcgcg cgcgccgcgg ttggtgacga cgcgctcggg gtggatcgtg
      301 ccggtcacgg catagccgaa catgatctgc gcgttgtcta cgctgtgccc gccgacgagc
      361 gccacgccgt tctcttcgag caccgagagt ccgccgcgca taatctcgcc gaggatttgg
      421 aagtcaacgc ccttcttcgg aaagcaggtg acggcgagcg ccgtgatggg caccccgccc
      481 atcgcccaca cgtcgttgat cgagttgagc gcggcgatgc ggccgtagtc gaaggggtcg
      541 tcaacgatgg gcgtgaagaa gtccacggtc tggacgagcg cgaggtcgtc gcggagacgg
      601 taaactcccg cgtcgtcggc cgtctcgatg ccgacaatca ggttcgggtc cgcggaaatc
      661 ttcaacccgc ccagcacctg ggcgagcacg ctcggcgcga gcttagacgc tcaaccggcg
      721 cacgaaacca tctcggtcag acgcataatc acctccgaaa aagtagtcag gagtcagaag
      781 tcgggagtca gaagtaggac tgatgtctga gcgattcagt aacgatgcaa ccgccgtttt
      841 cttacacccg aactcctgat tttctgcatc tcatcgtacc acgaaccttg cgtgcgcgac
      901 gacggcgcgc gcttcccgct gttggtattc gtggactgtg gggaggggag gcgcgcgccg
      961 cgcggcgcgg gcttttgtgt tcgcgggagt gagcctgtag tatagggccg cgcgcgccgc
     1021 ggcggacatt tttcagcttc ttttatggtg aggaagcgtg ttttaggaga acgatatctg
     1081 atgagagacg agaacgattt ttttgacgat ttgatcgacg acgaggcgga aacttatacc
     1141 tcggaagcct ccgacgagga cgacttcgag ggcgacgacg acgaagatga cgaggaattt
     1201 gagacgcgga cggccgtcct ctcgaagaag ggcatgctcg cgttgctcac cgtcaagacc
     1261 aacgaagacg gcggccagat cgtccgcgtg gacccgcgcc agcagctccc gaccgcgcaa
     1321 cgctacgaca cccccgagag cgccatcgag tggttccggc gcagcctcgc caccagccgc
     1381 cgcaacggtt gggaagtcgt ctacgacggc gcccccgcgc aaggttaact cccgcgggcg
     1441 gcgtcgccac cactcgcacc gccgacggcc gcgcggttaa cgattgtgcc ggccactaac
     1501 aacaaccccg aaacacgaac aggcaacgcc gccacagcgc tcgccctcac ggccttcgtc
     1561 gcactcgcgg cctatctctc agtcgcactc atccagccgc cgacgcccgc gcccattgat
     1621 gcgccgccca cggagttctc ttccgcgcgg gcgacggaac acctgcgcgc cgtcgcccgc
     1681 gagccgcacc cgacggggtc ggacgcgagc gtgcgcgtgc gcgagtacat cctgagtgag
     1741 ctggccgcgc tcggcatcag cgccgaagtg cagcgggcga cggccgtgtg ggagagccgg
     1801 cggcgcgagc agccttcgca ggcggcgacc gtccataaca tcgtcgcgcg catggccggc
     1861 tccgcgcccg gcgcaaaagc cctcatgctc gcggcgcact acgactcggc gccgacgggg
     1921 ccgggggcct cggacgacgg cgcgggcgtc gccgccatcc tcgaaaccgt ccgcgcgctc
     1981 aagacgggcg cgccgctcaa gcaagacctc atcgtgctgt tcacggacgg cgaagagctg
     2041 gggctgctgg gcgcgcgcgc gttcagcgac gagcacacgt ggatgcgcga tgtcggcctc
     2101 gtgctcaact tcgaggcccg aggacgcggc ggcccgtcgt tcatgttcga gacgagcgag
     2161 ggcaacgaag cgctcatcgc ggagttcgcg cgcgcggccc cgcgccccgt ggcgaactct
     2221 ttgatgtacg cgctctacaa gcgcctgccc aacgacaccg acctcaccgt cttcaaggcc
     2281 gccggcgccg ccggactcaa cttcgcttac gtctccgaca tcactcacta tcacaccgcg
     2341 ctcgacgcgc ccgagcgcgc cgatgagcgc agcctccaac accacggctc ctacgcgctc
     2401 gccctcgcgc gccacttcgg caacctcgac ctcgcgccgc tctcgggcgc gggccgcggc
     2461 gacgccgtct acttcaacgc gctcggcccc gtcttcgtca gctacccgga ggcatgggcc
     2521 gcgctgctga cggcagtcgt cgcgctcgcc ttcgcggtcg tcgtctgtgt cggctggagg
     2581 cgcgctcgtt tgagcgccgg gggagtcggc ctcggcctcg cgctgccggc cgtctgcgtg
     2641 ctggcggcag tcgtggcggg cgcgggcggg agtcggctga tcgcgcggct gcacacggac
     2701 taccgcgcgt tgcccttcgg cacgccatac gacgcgtggc ggttcgagtg ggcgctcgtc
     2761 ctgctcgtcg tcgcggtctg cgcggccgtc tacgcggcgg cggggcggcg tgcgggcgcg
     2821 tcgagtctga ccatcggcgc gatgctgtgg tggctcctgc tgctgttcgc gtcaacggcg
     2881 ctgttgcccg gcgggagttt cctgttcgcg tggccactac tcttcgcgct cgtcggcctg
     2941 agctacactt tctttggccg cagccgtgcg acgtggatgg agtcggcgat gctggccgcc
     3001 ggcgcggcgc ccgcggtcgt gctcggcgcg ccaatcgtct acatgctctt cgtactgttg
     3061 ggggcgggga cgagcgcgcc cgcactcgtg ctcgcggcgc ttctcgcgtc gttgctcttg
     3121 ccgctgctgc gtctgtcggc ggcgcgtggc gcgtggctgc tgagcgcggc ggccggcgtg
     3181 tcggcgttcg cgttcttcgt cgcgggagtc accggcgcgc gcttcgacgc ggagaggccg
     3241 cgcatcaaca gcgtcttcta cgagatgaac gccgacacgg ggcaggcgcg ctgggtgagc
     3301 gccgacgccg cgccggacgc atggacttcg cagttcttcg ccgcgaccgc gaggcgcgac
     3361 gtgtccgcct ccgtctttcc gtgggagacg ggcacggtgc tgaccggcga agcgcgcacg
     3421 ctcgacgcgc cgccgccggc gctacaggtt ctcgaagaca gggccgacgg ctggctgcga
     3481 cgcgtgcgac tgcgcatctc ctcgcagagg ggcgcgccgc tcatgctcgt ctacaccggg
     3541 gcggaggcgg aggtgcagag cgcggaggtg ggcgggaaaa agttggcggg cgctggagag
     3601 gcggggaacc agttgagggt gttctacaca ggcgtccccg tcgagggcct tgacgtgcgt
     3661 ctggaggtca acgcctctcg gccgctgaag ctgaccgtcg ttgatggcgc ttacgggctg
     3721 cccgaaacgt tcggcatcgc gccccgccca tccggcatga tgccgaagcc ttcaaagatc
     3781 ggcgacgtga cgctcgtcag gaaatctttc acgttatagt cggcggggct tgtcgctgct
     3841 gcttctttgg aagaaattaa catggatgga caagataagc aggaggaaga cgaatgaaac
     3901 attaatttga aatccgagat ttgagatttc aaatcttctt atcctgctta tcttgtccat
     3961 ccatgttaat tgttttgaaa gtggcgggcg gcggacgtgc cctgcccgca caaattgaat
     4021 gggcgttgag gagtaaaaaa tgaaagcgat tcgggttcac gagttcggcg cgccggaggt
     4081 gatgcgtctg gaggacgtac cggagccgcg cgcgggggcc gggcaggtct tggtgcgagt
     4141 gcgcgccgcc ggggtcaacc ccgtggacac atacgtccgc tcgggcgcct acgcgaagtt
     4201 gcccgagttg ccgtacacgc cggggtctga cgcggcaggg atagtcgaga tggtcggcga
     4261 gggcgtcacg cggctgcgcg ccggcgacag ggtttacctc gcgggaagtc tgacgggcgc
     4321 ctacgccgag cttgccctgt gcgacgagtc gcaggcgcac acgctcgccg agcgcgtctc
     4381 gttcgcgcag ggggcgggtg tctacgtgcc ttatgcgacc gcctaccgcg cgctgttcca
     4441 gcgggcgctc gcgcgtgcgg gcgagtgggt gctggtgcac ggggcgagcg gcggggtcgg
     4501 cgtcgcggcc gtgcagctcg cgcgcgcggc cggactcttc gtcatcggga cgggcggcac
     4561 cgaagagggc cgacgcctcg cgcgcgaaga gggcgcgcat cacgtgctcg accatcgcgc
     4621 gccggactat ctggagcagg ccgtcgcgct cacgggcggt cgcggcgtgg acgtgattct
     4681 ggagatgctc gccaacgtca acctcgaccg cgacctcggc gtgctggcgc ggcgcgggcg
     4741 cgtcgtcgtc atcggcaacc gcggctcagt cgaaatcaac ccgcgcgcgg ccatgatgcg
     4801 cgacgccgac atcctcggca tgtcgctcct caacaccccg cccgaagaac tgtccggcat
     4861 ccacgccgct ctcgtcgcgg ggctggaggc gggcacactt cggcccgtcg tcggacgcga
     4921 gttgccgctc gccgaggccg cacgcgcaca cgccgccgta ctcgaacccg gcgcctatgg
     4981 gaaaatcgtc ctggcgccgt gagaacgtcg tgtgacttcg tgaatcgtac cttgcttata
     5041 tttatgggat gggttctagt tatcgtggtg atgtcgtggt ctaaggcggc gtcgggtctc
     5101 ccgtcgggcg ggctgagact cggcgggtaa agggaggatg ggaggcttcc gctctaagtg
     5161 atcgagacag tatgccccga gcgccagcaa gggaaaggtg attgcgaaaa atacgttgct
     5221 taaaacgttc agggtcgggc cgccgccggc cggcgtggtg aaatgaacgg ccgcccacaa
     5281 cagcagtgcc agggggattg acagcacgcc gccggcgagt ccgcaggccg cgccgatgga
     5341 ttgccaccca ccccgcttct tgcttaggag aaggtgtatt ctgcggtata gctcatcccc
     5401 caggtcatcc tcccgcagcg tataaatttt gctcatgata ttccctttat ccgcgcgcga
     5461 tgaggggcgc gagagcatcg tgctcccgcg cccctcaaaa gtccggccgc ggcatcgttc
     5521 ctcactcagc acggcgaaca acttcggcaa acgaattgtc aacgcccgga tgttacgacc
     5581 acgcgtataa gaatggcgta aaggatttat aaaaagtttg taaagattag acgaggccgg
     5641 aggggcctgc ctatgagtca gagttgaagc ggtagccgac ccacggctcg gtgaggatat
     5701 agcgaggggt tgaagggtta ggctcgatct ttttacggag ctgtccgaca aacactcgca
     5761 ggtactcgcc ttgctcggtg tagttgcctc cccagacggc gccgagcagc gtgtggtgcg
     5821 tgaggacttt gccgacgttc ctcacgaagt gaacgagcag ctcgtactcc ttgggcgtca
     5881 gatggacttc cgctccgcgc acgaagacgc tgcgggcttc gaggtctacc cgaaaatcgc
     5941 cgacttctaa aaccggggaa gcttcacttt tggggagcgc cgcagttcgg cgcagttgag
     6001 cgcgcacgcg cgccagcaac tcgtccatcc cgaagggctt ggtcacgtag tcgtcggcgc
     6061 cggcgtccaa agattcaacc ttgatctttt cttccccgcg caccgagagg acgatgatcg
     6121 ggatttggga gacctgccgc aggcgtcggc agagttccag cccgtcgagg ttcggcatcg
     6181 agaggtcagt cacgacgagg tcaggattcc agtcgttgaa agtctccagc gccgagaggc
     6241 cgtcggccgc gacgcgcacg tcgtagccgt gtgtcttcag ccccgtgcgc aggacgcgcg
     6301 cgatctgcgg ctcgtcatcg accacgagga tgcgcgacgg ttgggtcatg acactcggtt
     6361 caactccgcg gaaggggccg tctcggaaac cacctccggc gaaggctccg gtatttcttc
     6421 cgcatcgccg acagggaggg tgaacgagac gcgtgtcccg gcgccttccc ggccgtcttc
     6481 aatccagatg cgcccgccgt gagcttccac gatccccttc gcgaccgcca gtcccatccc
     6541 ggttcccttc agtctgcggc cgtcgccggc gtcgccatcg cgcgtcgcgc ggaagaactt
     6601 ctcgaagaca cgctcgtgca aatcgcgggg gatactttcg ccctcgttct ctaccgcaat
     6661 gcggatcgcg cggtcgtcgg cgcgttccgc ggcgacgagg atggtcgtgt ccgccggcga
     6721 atacttcgcc gcgttatcga ccagcgtaaa aatcacttcg gacacggcgc gcgggtctac
     6781 ctgcacgacg ggaatttcgt cctcaatgtt gacccgaact tcatgacgcc gcgtcagcgg
     6841 ttcggcgcgt gtgagcgccg ccgtgatgat ctcgtcaacg gccccccacc tgcgccgcaa
     6901 ctgcaactcg cccgcttcga tgcgggccag atcgatcagt ccctcgacga agcggttgag
     6961 ccggtcgcat tcctcgtcga tcacctccag catctcccgc cgcgcctccg cgtcgagggc
     7021 caccgactcc cggcccggcg tccgcgcacg cagctcttcc aacagggtgc tgatcgacgc
     7081 tttgatggac gtgagcgggg tgcgaatgtc gtgggtcacg gcgtcgagca gggccgactt
     7141 cagccgctcg ctctggcgga gcgcctcggc gtggctcgcg cgctcgaagg cgtcacgcaa
     7201 ctccgtgtaa agccgttcta tctcctgccg cccggcgtcg gcctcttcag tgcgccgctt
     7261 cgcgtgcgcc gagagctgcc ccacggtgac ggcggtgatg agaaaggcga cgagcgcaat
     7321 cgagttgtcc caggcgtcaa tcgtgagcgt gccgtatggc ggcaggaaga agaagttgaa
     7381 gcataggacg ccaatgagcg aggcggccac agccggccgc gccccccacc cggtggcgac
     7441 gaagagcacg acgagcagga gggcgagcgc gaccgtggcg gcgttgatgt gtgcggcgtt
     7501 gatgtgcccg gcggacagct tcagcgcggt cgtgacggcg gccacgcccg cgacggccgc
     7561 cacgtaaccc atccgactct ttccgaacgc catcggcctc atgcttccta ttgtacgaca
     7621 ggaaggtaag agatgtgggc cgtgcccgtc gcgcgctaaa tctcgacctg cgaacccatc
     7681 tcgacgacgc ggtcaggcgg caggcagaag taggcggtag caggcgaagc attgcgcgac
     7741 atgagagcga acagcttttc gcgccacagc atcatgccgg ggtgcttgcc cgcgatgacc
     7801 gtctcgcggc cgagaaagta agtcgtctcc gccgggtcga aggcgaggcc gggcgtctcc
     7861 atgctcgcca gcacgctggg cacgtccggg tcttccataa agccgtagtt gatgacgacg
     7921 cggtaaaact cgtggccaag cgcctgcaca ctcacccgct cttccggctc gacatgcgga
     7981 acctgcctcg tcttcaccgt caggagcaga acccgctcgt gcagcacctt gttgtgttcg
     8041 aggttgtgct ccagcgcggg cggggtgccc gcccccgtgc cgttcatgaa gacggccgtg
     8101 ccgggcacgc gcaccggggg agtcttggcg atgtcgcgga ggaaatcttc aatcggaata
     8161 gcaagcgaca acatccgcga ggcgaggatg cgtcggccct tcttccacgt catcatgaat
     8221 agaaagattc ctgccccgac gacgagcggg aaccagccgc cggccggaat cttgatgatg
     8281 ttagccccga agaacgcgag gtcgatgacg aggaaaaagc ctgaaagccc gagcgtcagg
     8341 ggcagccccc aaccccactt ctcgcgtgtg acgacgccga gcaggatggt cgtgatgacc
     8401 atcgtcgccg tgacggccac gccgtaggcg gcggccagat tgctcgacga gccgaagccg
     8461 atgacgatgg cgatgcagcc gatcatcagc gcccagttga gcgccgggat atagatttgc
     8521 cccatctcgg tcgaggaggt gtgctcgatc ttcaggcgcg gcatgaagcc gagttggatg
     8581 gcctgcatcg tgagcgaata cgcccctgag attaaagcct gcgaggcgat gatcgcggcg
     8641 caagtggcca gcgcgatgag cgggtagagc gcccactcgg gggcgaggcg gtagaaggga
     8701 ttctcggcga ccgccgggtt ctctatgagc agcgccccct gaccgaggta gttgagcaga
     8761 agagcgggca ggacgagcgc gtaccagacg atgcgaatcg ggcgcttccc gaagtgtccc
     8821 atgtcggcgt aaagagcctc gccgcccgtc acgacgagga agaccgtgcc gagcacgagg
     8881 aatccgtgcc agccgttgcg gataaaaaaa ctgaagccgt ggagcggatt gaacgcaccg
     8941 atgaccgacg ggtgtcggac gatctgcgcg acgccgagcg ccgagatgac gaagaaccac
     9001 gcgagcatga cggggccgaa caggcgaccg actttcgccg tgccacggct ctggatgagg
     9061 aacaggccga cgatgatcac gaccgtcgcg ggcacgacgt aaggggcgaa gagcggcgtc
     9121 gccacgttca agccttcgac agcgccgagg acggagatgg cgggcgtgat gataccgtcg
     9181 ccgtagagga gcgccgcccc gaagatgccg agcacgacga gccacttgcg ctcgctcttg
     9241 ctcacgatct tgatcggcgt cgccagtgcg gtgagggcga ggatgccgcc ctcaccgtgg
     9301 ttgtcggcgc gcaggacaaa gacgcagtac ttgattgaga tgatgaggac gagcgcccag
     9361 aagatcagcg acagcacgcc aaaaatattc tcgggcgtgg gcgcaatcgc gtgcgggccg
     9421 tgaaagcact cgcgcatcgc gtagagcggg ctggtgccga tgtcgccata cacgatgccg
     9481 agcgcggtga gcgagaggat ggccagatag cgaccccgcg gcttggcggt gtgtgggtgg
     9541 ccttgcgcgt ccgtgagtgt cgtgtggata tctgccgagg ctgttcctgc catagtcgtc
     9601 ctgaaagaaa agaatcgttg ctcgtgaaac ggttaccgcc cgaccgcggc gaaggagacc
     9661 gaaggccgtc cctccatggc ccttcgtgtc acgacaatca aattgggcgg acgaattatt
     9721 gctgctggga aaaccagcgg ggttaggatg gcataaaagt tataaagatt ttgtaaagat
     9781 gccgccgacc ttcgttgatc tgatgcaggc gaattgccgg ccgtcagaca cccgcagacg
     9841 cttgtaaatt cttgtaaacg gatgtcaata agacgtaaaa agcgcccggt cgagtgctga
     9901 cgaccgggcg cgtttgttgt atgttatgcg cgtgcccgtg tcggggcctc cgcagtatcg
     9961 atgttaaaag tccgcacatt cttccgcaag cacatcctgt gggtggggct cgtggcggtc
    10021 gtcgtcccgc tgctgagcat cctcgcggtg cagtacaact cactcaagaa gctggagaag
    10081 acttcgagcg tcgccgacaa gatgtggatg aagaactacc tgagcgacgt ctcgaaggag
    10141 gtcaagttct tctaccacac gaacgccgag caggtcttga acgtcccggc ctattccgtc
    10201 tcggccgaga acctgcacaa ggtacgcaac cacttcggca agtgcgaggt cgaggggtcg
    10261 aagcgcctct tcatcgtcgc gttcgacgag ggcggcggct cgcagacgct cttcttcgac
    10321 ccgcgcgccc aggcccaact cgttgaggcc acgcccgccg aggtgcgcgc cgccaacatc
    10381 gtcgccgcgc cctacaagat gctcagccag gagggcacgg ccgtggcggg cgcgaagatt
    10441 tccgaagacc tgcgcgaccc ccagaaccgc gtcatcttca agcccatcac cgacgagtgg
    10501 gcgcgcgtcg tcggcgtggc cgggatgatc ttagacaccg agttcttccg cgagaagttt
    10561 ttgcctcgcg tcatcgccgc gacgctgccg aagttcttcc ccgacgaagc gcgcgacaac
    10621 gtcatcgtca ccgtctacga ctccgagcag aagctcgtcc acgccacgca agccctcaag
    10681 gggcagaacg acgagatcgc caccaacctg cccttcttct acgactatcg tctcggcatc
    10741 cgcagccgcc acatgacgcc cgagcagtgg gcgcgctgga acttcaactt caacctgacg
    10801 ctctcgctcc tgatgacgct cgtgctggtg agcggcatcg tcctcgcgct gcggatggcc
    10861 tcgcgtgaga tgcacctgtc gcagatgaag accgacttcg tctccaacgt ctcacacgag
    10921 ctgcgcacgc cgctctcgtc catccgcgtc ttcggcgagt tcatgaagct cgggcgcgtg
    10981 cgcgacccgg agaagatgcg cgagtacggc gaacacatcg agaccgagag ccggcgtctg
    11041 acacagctca tcaacaacat cctcgacttc tcgaagatcg agtcggggcg caagacctat
    11101 cgcgtggagc aggtctgtgt ggaggagttg atcgccgagg ttttgaagac gtgcgaggtg
    11161 cggctgcgtc agaacgactt cgcgattgtc tacgagcctt cgtcgcagcc gctgcccgcc
    11221 gccttcatcg accgcgacgc catcgagcag gcgctgatga atctgctcga caacgcggtc
    11281 aagtactccg agtcggccga ggtcagggag gtcgtcgtcc gcgccggcta cgggggcggc
    11341 cttatccgca tctccgtgac ggatcacggc gtgggcatcc ccgcctctga gcaggggaag
    11401 attttcgaga agttctaccg cgtctcgacg gggctggtac acgacgtgaa ggggagcgga
    11461 ctgggtcttt cgctcgtcaa gcacatcgtc gaggcgcacc gggggcgggt gacggtcgag
    11521 agcgagccgg ggcgcggcag cacgttcacc atcctgctgc cggcggcggg cggcgcggcc
    11581 gggcaggagg agacgccgca gccgacggac gccattggcg gcgagcagcc gcttaacctc
    11641 gggtacaagc actgatgtgg gccgcgacct gagacaaact tgattttatg gatagtagga
    11701 ctcaccgcag aggcgcagag gacgcagagt tcacgccgca gggttgagtt cggttcaagc
    11761 ctctgcgcgt cctccgcgtc ctctgcgcct ctgcggtgag ttgccatacc caatgctcaa
    11821 cacgaagcca aactgatcct tttgaacttc gtactcatca aggagagcgt atgaaaaagt
    11881 tgatgctgtt agccgtcgtc acggccgccg cggggctggc cgcgtggctc ccggcgcgcg
    11941 cgcaatcggc cgaggaggcg gccgtccgcg ccgccatcga acactatttc cgcgcccacg
    12001 caacgggctt gggcgagcac caccgtaagg tcttccaccc cgacgcccgc ctcttcgccg
    12061 tccgcgatgg caagtactgg acgctcacca gcgaggagta catctcgcgc gcgccgggca
    12121 agccgcccgc cgacgaggcc gagcgcaagc gcgccgtcga gatggtggac atcgccggca
    12181 acgcggccgt cgccagagtc gtcctcgact accccgacgt gaagttcacc gactacatgt
    12241 cgctcctcaa gatcgacggc gaatggaaga tcgtcaacaa gacgttccac gccgagccga
    12301 agactaaaaa atgaggtcgc tccccaagat cgatgggcag tggcagatcg tcaacaagac
    12361 cttctcggaa caacgccgtc gataggaagg ttggcgacaa gtctccacta gcggcccgag
    12421 gctttcacct gcgcccctaa agcctcaagc gcaagtcgct tctcggcaaa gccgaacgta
    12481 cacacggtct gaggataagc tgcgggtcta
//
LOCUS       DASVES010000277        15742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_774760, whole genome shotgun sequence.
ACCESSION   DASVES010000277 DASVES010000000
VERSION     DASVES010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774760"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..2718)
                     /locus_tag="VF240_11115"
     CDS             complement(112..2718)
                     /locus_tag="VF240_11115"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX7175800.1"
                     /translation="MRPRLTLQLALLFATVIATLVALATLKPPTAAAQSSPQTPGALQ
                     IVGPDGKAAGSCPLKHTDVRAEISGFLSRVTVTQEFENPHKDKIEAVYVFPLPQMAAV
                     DDMTMTVGERTVKGKILPREEARTVYEAARATGRVAGLLDQERPNIFTQSVANIMPGE
                     RVTIKISYVETLKYDEGTYEFSFPMVVGPRYNPGSPTGRTSSGFAPDTDRVPDASRIT
                     PVPESPGTRAGHDISVEVALDAGVPLDSLRSTTHEVDVERVGASGARVRLKNHSTIPN
                     KDFILSYDVAGRKVSDALLTHRGERGGFFTFILQPPDRVTAADVTPKELVFVLDTSGS
                     MSGFPIDKAKEAMRHALAGLYPQDTFNLITFAGDTHVLFPAPVPATPENLSRAQRFLE
                     TRSGGGGTEMMKAIKTALDPSDSQSHVRIVCFMTDGYVGNDMEIVSEIQKHPNARVFS
                     FGIGSSVNRFLLDKMAEAGRGEVEYVALNDDGSAAAMRFHKRVREPLLTDISIEWGGL
                     NVADIYPRRIPDLFSAKPVVVTGRYVGNGRGRLRLTGRMSGKHFEREIPVELPEAEAR
                     HDVLATLWARTRIDDLTGQDYAGIQQGNARPEIRQEITQLGLEFRLMTQFTSFIAVEE
                     MTITDGGPPRRVDVPIEMPEGVNNLQLDGTQNAVVSSNFSIQQIQSLPMSARKVSNTM
                     RASRPAAKGGVLPVGSITETVTVTAAESHIDMTSSSATTTVYPPPAPPGDRKDKPGRE
                     EKQAAGTGEGRGASERVAGPTAEEVRRQQIIAKMHASLAAVVERLWKNDAKPGAEEVK
                     FVREGKAEVQVWLTDKSDATMAKLKALGFEIILDPKSGKVVIGRLPLEKLVALAEIKE
                     VRYVAPQTAGGR"
     gene            complement(3140..3823)
                     /locus_tag="VF240_11120"
     CDS             complement(3140..3823)
                     /locus_tag="VF240_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175801.1"
                     /translation="MKAALTRGVVCFVLAAACVCEAPVGGPALASNGAAAEKVRPAGD
                     VVVVLNEDFFNALLEAIMEQPGGAKFPLSKGGSSKCAGEIALMRESGGRRTAARFDAG
                     RVGATVAFRGSYEAPLVGCLNFEGAADTLFHLAFDAERQAFVARVEVRNIDLKNVPAM
                     MGGGITGLVQDAIDARINPVEILRADQLAARLPLAQGNALRLRAKEVRHEVAGKELRL
                     RIVYEIVSE"
     gene            complement(3884..4864)
                     /locus_tag="VF240_11125"
     CDS             complement(3884..4864)
                     /locus_tag="VF240_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX7175802.1"
                     /translation="MEESYAAQVSELLHMEAEAVRRAAERLDAAAVERAVRLLAACRG
                     KVVLVGVGKSGNIAQKIAATLTSTGTAALFLHPSDAQHGQLGIVGREDVVVVLSNSGE
                     TAEIVELLPHIQHRRASVIALVGNLDSTLARRADVALDASVEQEACPLGLAPTTSTTV
                     ALAMGDALAMTLMRVKGLTPEDFALNHPGGRLGKRLTLRVGDLMHAEAAHPTVRPDAA
                     LLDAVREIGRGGLGAVSVVDGGGLLVGIITDGDVRRTLQRGRLADLEDLKCGDIMTRD
                     PVVVAPDALAYAALRLMEDRPSQISVLPVVEADGRAVGLLRIHDIVRSGI"
     gene            5092..5706
                     /locus_tag="VF240_11130"
     CDS             5092..5706
                     /locus_tag="VF240_11130"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX7175803.1"
                     /translation="MENEQLYRVALVALYLLFHALRGHFAQGVLRTGGKVFSKRDDDT
                     HEGRLHSSAGLLMEIILPLSVIVYAVYPAWVARLALPFPQLVRLLGVGLCLVSLVQLV
                     RVHRALGRHWSASLRLRDDHRLVTEGPYRRVRHPMYTALIGNMLGLCLMSANLLIIVP
                     RLVQIFLLLLRMGKEESMMLSRFGEEYRSYMARSGRLFPPPHAR"
     gene            5867..6490
                     /locus_tag="VF240_11135"
     CDS             5867..6490
                     /locus_tag="VF240_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX7175804.1"
                     /translation="MARFSFLPKEEQYFALLKQMTSYIYDAAHGLTELMADQTGDYTK
                     HARHIKTIEDSCDDLIHSIVTKLNKSFITPFDREDIYTMAGALDDIVDLIDDSARAMV
                     IFNVRESTEHAREFADVILRMAVQLHEAISALERPADIGPRLVELHRLENEGDDIYHT
                     AIAELFRDTADPLTVIKWKEIYEKLESAVDRCESVANIIESIIIKHA"
     gene            6606..7631
                     /locus_tag="VF240_11140"
     CDS             6606..7631
                     /locus_tag="VF240_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7175805.1"
                     /translation="MDPALVFVIIIVVVALVFDYINGFHDAANSIATVVSTRVLSPGA
                     AVVWAAFFNFIAFLVLGEAVAKTIGKDMIDIKAIPGDWQLWVILCGLIGAIVWNLITW
                     YYGIPSSSSHALLGGYAGAAVAASGLGVILPNGWTKTLIFIILSPLIGALLGFIIMVA
                     VSWIFRRWSAHGVDKFFRKGQLASAALYSLGHGGNDAQKTMGIIAGVLVASNLDSTAT
                     TDIPLWVVLICHAAIGLGTLSGGWRIVHTLGSRITKLKPVGGFAAETAAAMSLIGATY
                     GGIPVSTTHTITGAIVGVGATRRLSAVKWGVAGRIVWAWVLTIPAAAGIAALSFLAYR
                     LLAPYFA"
     gene            7762..7977
                     /locus_tag="VF240_11145"
     CDS             7762..7977
                     /locus_tag="VF240_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175806.1"
                     /translation="MMSEQKRAGWIVPENQTSGHHTDSSKTNPTRPGAGEEHFEEGRS
                     QPGVAGQGADQLSDADRARLKDVNRKS"
     gene            8105..8959
                     /locus_tag="VF240_11150"
     CDS             8105..8959
                     /locus_tag="VF240_11150"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX7175807.1"
                     /translation="MRWRDRAGSANVEDRRGMGRGGMAVGGGIGTLAIVLLAAFCGVD
                     PSALLNQLPQEGGGAPGPQAERRVNPEEDELAQFTRVVLRDTEETWNEVFRQAGRQYR
                     EPTLVLFSGQVQSACGLAGAAVGPFYCPGDQKLYIDLSFFRELRARFRAPGDFAQAYV
                     VAHEVGHHIQHLLGTMDKVHAAQQRMRGAEANQLSVRLELQADFYAGVFAHHVQRKGY
                     LEAGDVEEALNAATAIGDDKLQKEGQGYVVPDSFTHGSSEQRMRWFRKGLETGDIRQG
                     DTFSTRSL"
     gene            complement(9223..12531)
                     /locus_tag="VF240_11155"
     CDS             complement(9223..12531)
                     /locus_tag="VF240_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7175808.1"
                     /translation="MLRRLSVCLLTFLVVFGAQGVSAQEPANKPGAVQDSQKKPDTAP
                     EAKKNEPWDVEGDHGPSRIVEFDTDEGTWMSVDVSPDGQWLAFDLLGDIYRMPAAGGR
                     AELVASGVSFEAQPRFSPDGKLVAFTSDRDGVENIWVMDADGKNRRQVTKETTRQMST
                     PAWSPDGHYLFARKHLTDTRSAGAGEVWMYHIQGGGKGVQITEKPNWTANIGEPEVDP
                     TGRFVYFVASNAFDYNKNVYENIYWIDRYDLQKGRRSTFVRGSGGSVRPQISPDGKLL
                     AFIRRVGLKSVLHLREVETGREWPVFDGLTRDQQETWALFGTYPGYSWTPDGKSIVIY
                     AKGKIVRIDVNTRAVTPIPFTAHVKQKVSEPVRFRQKVAPERDTARLLRWARSTGERV
                     VYSALGKIYVKEGAQEPRRLLNSKYFEYAPSFRPDGLMITYVTWSDEEKGAVWVANAD
                     GSNQTKITTTADQYANPVFSQDGTKIAYLKGRGSIYHEADLASESQFEIHYWAAGKHN
                     YVMDLQSRGPNARMPVLSFDPAGERIFFMEAGSGGGGAPTQPAQFITYLSSVKLAGDD
                     SRRHIESNYVTEIIPSPDHEWVAFKELHKLYVAPFPKAGKTLKLSSTEATVPVKPISK
                     ESGDWLTWSPDGTSVQWTLGENFYEQSLTNIQKQLAKDEKTPEPRATRIGFEFETARP
                     RGTVALTNARVITMRGDEVIARGTVVIKDDRIVSVGARVAVPAGARVFDMRGKTVMPG
                     FVDVHAHMGYNTLDIIPERQWPYWVNLAYGVTTTHDPSASTQLVFSQSEMVKSGAMTG
                     PRVFSTGYILYGAENPEKAVVNSYEDARSHLARLKAVGAFSVKSYNQPRRDQRQRIIK
                     AARELQMMVVPEGGSTYYFNMGHVLDGHTGIEHAIPVAPLYKDAVTLLARSGAGYTPT
                     LIVGYGGVWGENYWYQHSNVWENEKLLRFTPRRIIDERSRRRMMAPEDDFFHFELSKS
                     VKDVVRAGGRAQLGAHGQLQGLGVHWELWMLGQGGLTPLESLRAATLHGAAYLGLDAD
                     LGSLEPGKLADLIVLDKNPLENLRHSESITHVMIGGVLYDTRNMDEVYPVERKRTPFF
                     WERRGETPTEGRNN"
     gene            complement(12617..12982)
                     /locus_tag="VF240_11160"
     CDS             complement(12617..12982)
                     /locus_tag="VF240_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7175809.1"
                     /translation="MIVVNRRQAAVINTPHGSEIRPLIDRTTSRVELCSLAEETLPPG
                     AAVGRHFHTATEEVYYITAGRGRMTVGAETREVGPGDAVYIPRGSAHTLENTGAEPMT
                     ILLVCGPAYSVADHLTSDE"
     gene            13132..13204
                     /locus_tag="VF240_11165"
     tRNA            13132..13204
                     /locus_tag="VF240_11165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13165..13167,aa:Arg,seq:ccg)
     gene            13351..13806
                     /locus_tag="VF240_11170"
     CDS             13351..13806
                     /locus_tag="VF240_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7175810.1"
                     /translation="MKVFDILRQDHQKVADIFQQLEPAADDSTRQQLFTQLKQELDLH
                     AHVEETILYPALKDASQTREIILEAYEEHAEVKELLARIEQTPPADAKWADLLAELRH
                     NVEHHVDEEESEMFDAARDVLSGAQIDDITRRVAEAKQQGLKAADAGSL"
     gene            13984..15717
                     /locus_tag="VF240_11175"
     CDS             13984..15717
                     /locus_tag="VF240_11175"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7175811.1"
                     /translation="MHRFRLKGLTLALLMLLPAVPLPPVRAQQQQQAQSAAAATPAAA
                     QAKRLALTTSSAARKAAERITAAQMKEDLYWVADDARGGRDTPSAGLDATAKFIADRL
                     AKARIKPAGDDGTYFQKIELRSTSVDSEKTRVQLAGRAFKLGDDFVIASTASAEASGP
                     LVYVGHGWVVRSKNINAYEGLDVKNKIVIVAGAGVAPPEGVTPEYIAGRPASDWESPL
                     SYAEKNGARGVVFVPKNHERIYQFYRRSLGRSSYQVKRFVEESEGRAGAPPSNIPVII
                     PTAEMLTALFEGERTAGADIMKAANANAGDQGFDFPAAKTMSIATSVKVSEATTQNVV
                     GVIEGSDAKLREEYVAVGAHYDHVGMRPGQSGDNIFNGADDDGSGTVAVLSMAEAFAK
                     ARSPKRSVLFVWHAGEEKGLWGSQYFTKFPTVPIKQIVAQLNIDMIGRSRAPDDIKPA
                     NKGLSGPDEIFVIGSKMMSAELGEASEAVNRSYLNLKYNYQYDAPDDPEQLFYRSDHY
                     NYAVQGIPIVFYFDGIHEDYHKVTDTPDKIDYQKMQKVTRTVFVLATELANAPTRPVV
                     DTPLPVSSRRR"
BASE COUNT         2664 a         5238 c         5043 g         2797 t
ORIGIN      
        1 cggagttggc gcagagggtt gaatctctct gcgcgaactc cgcgccctct gcgactcggc
       61 ggtgaatcta tgtcgccatc actcaagtaa acaccgctct aaatgacgcg atcacctgcc
      121 gcccgccgtc tgcggcgcga cgtacctcac ctccttgatc tccgcgagcg caaccagctt
      181 ctccagcggc agacgcccga tgacgacctt gccgctcttc gggtcgagga tgatttcaaa
      241 ccccagcgcc ttgagcttcg ccatcgtcgc gtccgacttg tccgtcagcc agacctgcac
      301 ctcggccttc ccttcgcgga cgaacttcac ctcctccgct ccgggctttg cgtcgttctt
      361 ccacaggcgt tcgaccacag ccgccagcga cgcgtgcatc ttcgcgatga tctgctgacg
      421 cctgacctct tcggccgtcg gccccgcgac acgctcggaa gcgcctcgac cctcgcctgt
      481 gccggcggct tgcttctctt ccctgcccgg cttgtccttg cggtcgccgg gcggcgcggg
      541 cggcgggtag acggtcgtgg tggcggaaga cgatgtcatg tcgatgtgcg actcggctgc
      601 cgtgacggtg acggtctcgg ttatgctgcc caccgggagg acgccgccct tcgccgcggg
      661 acgcgaggcc cgcatagtgt tcgaaacttt gcgtgcgctc atcggcaacg attgaatctg
      721 ctgtatcgag aagttagaag acaccaccgc gttttgcgtc ccgtcgagtt gcaggttgtt
      781 gacgccctcc ggcatctcga tggggacatc gacgcggcgc ggcgggccgc catccgtgat
      841 ggtcatctcc tcgacggcga taaatgaagt gaactgtgtc atcagccgga actccagacc
      901 gagctgcgtg atctcctgtc ggatttccgg gcgcgcgttg ccctgctgga tgccggcgta
      961 gtcctggccc gtcaggtcgt cgatgcgcgt gcgcgcccag agcgtggcga gcacgtcgtg
     1021 ccgggcctcg gcctcgggca gctcgaccgg aatctcgcgc tcgaaatgtt tgcccgacat
     1081 gcgtcccgtg agacgcaggc ggccgcgccc gttcccgacg tagcggccgg tgacgacgac
     1141 cggcttggcg ctgaagaggt cggggatgcg acgcgggtag atgtccgcga cgttcaggcc
     1201 gccccactca atcgagatgt ccgtgagcag cggctcgcgc acgcgcttgt ggaagcgcat
     1261 ggcggcggcc gagccgtcgt cgttgagcgc gacatactcg acctcgccac gccccgcctc
     1321 ggccatcttg tccaggagga agcggttgac cgagctgccg atgccgaacg agaagacgcg
     1381 cgcgttcggg tgcttctgaa tctcggagac gatttccatg tcgttgccga cgtagccgtc
     1441 cgtcataaag cagacgatgc ggacgtgcga ttgcgagtcc gacgggtcga gcgccgtctt
     1501 gatggccttc atcatctcgg tgccgccgcc gcccgagcgc gtctccagaa acctctgcgc
     1561 gcgcgagaga ttctccggcg tcgccgggac gggcgcgggg aagaggacgt gcgtgtcgcc
     1621 cgcgaaggtg atgaggttga atgtgtcctg cgggtagaga ccggcgagcg cgtgtctcat
     1681 cgcctccttc gccttgtcga tggggaagcc ggacatggag ccggaggtat cgagcacgaa
     1741 gaccagctcc ttcggcgtca cgtcggcggc cgtgacgcga tcaggcggct ggagaatgaa
     1801 ggtaaagaag ccgccgcgct ctccgcgatg cgtgagcaag gcgtctgaga ctttgcggcc
     1861 cgccacgtcg tagctcagga taaaatcctt gttcggaatc gtcgaatgat tcttcagacg
     1921 cacgcgcgcg cccgaagcgc ccacgcgctc cacgtcaacc tcgtgcgtcg tcgagcgcag
     1981 cgaatcgaga gggacgcccg cgtcgagcgc gacctcgacg gagatgtcgt ggccggcgcg
     2041 cgtgcccggc gactcgggga cgggcgtgat gcgcgaggcg tcggggacgc ggtcggtgtc
     2101 gggcgcaaac ccgctgctcg tgcgtcccgt cggcgagccg gggttgtaac gcggcccgac
     2161 gaccatcggg aaggaaaact cgtaagtgcc ctcgtcgtac ttgagcgtct cgacgtagct
     2221 gatctttatc gtcacgcgct cgcccggcat gatgttggcg accgattgcg tgaagatgtt
     2281 gggccgctcc tgatcgagca ggccggcgac ccgccccgtg gcgcgcgcgg cctcgtagac
     2341 tgtgcgcgcc tcctcgcgcg gcaaaatttt acccttcacc gtgcgctcgc ccacggtcat
     2401 cgtcatgtcg tccacggccg ccatctgcgg cagcgggaac acgtagacgg cctcgatctt
     2461 atccttgtgc ggattctcga actcctgcgt cacggtgacg cgcgagagga agccgctgat
     2521 ctcggcgcgc acgtcggtgt gcttgagcgg gcaggagccg gccgcttttc cgtcggggcc
     2581 gacgatctgg agcgcgcccg gcgtctgtgg gcttgattgg gcggcggcgg ttgggggttt
     2641 gagagtggcg agggcaacga gcgtcgcgat gacggtcgca aatagaagcg cgagttgcag
     2701 agtcagtctg ggtcgcatga gttccctcct cagttgtgcg ttcaaggagg cgcgccaggg
     2761 agacggcttc gcgccggcag acttgtcctg taccgtgcgc ctcttcgagc ggcgccgggc
     2821 ggacgtggtg tgttggcact ctgacgcggg gcgcgcgcac aaggttgttt cagtggaagg
     2881 aacgaaattg atttcggtaa cttgcgcccc gagctgggac ggtgtgacgc gcgtctggag
     2941 tgacgtttac ttgaaggagg acgaaagtag attcaccgca gaggcgcaga ggacgcagag
     3001 ggggcgccgc aggcttgacg agattcatcg atgatttagc catccaaccc tctgcgtttc
     3061 ctctgcgtcc tctgcgcctc tgcggtgaac cgccgcacct tacactcaac cgaacactgc
     3121 tacaggcggg gatataacat cactcgctga caatctcgta gacgattcgc agtcgcagct
     3181 ccttgccggc aacttcgtgc cggacttcct ttgcgcgcag gcggagggcg ttaccctgcg
     3241 cgagcgggag ccgggcggcg agctggtcgg cgcggaggat ttccacgggg ttgatgcgcg
     3301 cgtcgatggc gtcctgaacg agccccgtga tgccgccgcc catcattgcc ggcacgttct
     3361 ttaaatcaat gttgcgcacc tcgacgcgcg cgacaaacgc ctgtcgctcc gcgtcgaagg
     3421 cgaggtggaa gagcgtgtcg gccgcgccct cgaagttgag gcagccgacg agcggcgcct
     3481 cgtaggagcc gcggaaggcc accgtggcgc cgacgcgccc cgcgtcgaag cgcgcggctg
     3541 tccgccgccc gcccgactca cgcatcagcg cgatctcgcc cgcacacttc gacgagccgc
     3601 ccttcgacag cggaaacttc gccccgcccg gctgctccat gatggcttcg agcaacgcgt
     3661 tgaaaaaatc ctcgttcagc accaccacca cgtcgcccgc cggccgcacc ttctccgccg
     3721 ccgcgccgtt cgacgccaac gccggccctc ccaccggcgc ttcgcacaca cacgccgccg
     3781 cgagcacgaa acaaacaacg cctctcgtca aagctgcttt cattcactcc tccaggaagg
     3841 ggccggctga tttcggtttg ctgtaggaca ggcattcctg ccttcatatt ccgctcctga
     3901 cgatgtcgtg gatgcgcagg aggccgacgg cgcgtccgtc cgcttcgacg acgggcagga
     3961 cggaaatctg cgagggcctg tcctccatca aacgcagcgc cgcgtaggcg agcgcgtcgg
     4021 gcgcgacgac gacggggtcg cgcgtcatga tgtcgccgca cttcaggtct tcgaggtcgg
     4081 cgagccggcc ccgctggagt gtgcgccgca cgtcgccgtc cgtgatgatg ccgacgagga
     4141 ggccaccccc atccacgacg ctcacggccc ccaacccgcc gcgcccgatc tcgcgcacgg
     4201 cgtcaagcaa cgcggcgtcg gggcgcacgg tcgggtgagc ggcctcggcg tgcatcaggt
     4261 cgccgacgcg cagcgtcagg cgtttgccca gacgcccgcc cggatggttc agggcgaagt
     4321 cctcgggcgt caggcccttc acgcgcatca gcgtcatcgc cagcgcgtca cccatggcga
     4381 gggcgaccgt ggtgctcgtc gtcggcgcga gacccagcgg gcacgcctcc tgctcgaccg
     4441 aggcgtcgag cgccacgtcg gcgcggcgcg cgagcgtcga gtcgaggttg ccgacgaggg
     4501 cgatgacgct cgcgcgccga tgctggatgt gcgggaggag ttcgacgatc tcggccgtct
     4561 cgccgctgtt gcttaagacg acgaccacgt cttcgcggcc gacgatgccg agttgcccgt
     4621 gctgcgcgtc cgaggggtgg agaaagagcg cggcggtgcc ggtgctcgtc agcgtggccg
     4681 cgatcttctg cgcgatgttg cccgacttgc cgacgccgac taacaccacc ttgccgcggc
     4741 aggcggccag caaccggacg gcgcgctcga ccgccgccgc gtccagacgc tcggcggcgc
     4801 ggcggactgc ctccgcctcc atgtggagca gttccgagac ctgcgctgcg taagattctt
     4861 ccattggttc tacggggccg gagttgttcg gaggtatact cgcgcgcgac acaggagaat
     4921 acgacgcggg agcgaccgag gacaaccgcc gagattcacg aacatgcaga gagattcaac
     4981 cctgcggcgt gaactctgcg tcctttgcgc ctcttgcggt gaatctactt cgctttcact
     5041 caagtgaaca tcgctctaac tgcccacgtt tgccaacgcg cgagaggtca catggagaac
     5101 gaacaacttt accgcgtcgc cctcgtcgcc ctctacctgc tcttccacgc cctgcgcgga
     5161 cacttcgcgc agggcgtgct ccgcaccggc ggcaaggtct tctccaaacg tgacgacgac
     5221 acgcacgaag gccggcttca ctcgtccgcc ggcctcctca tggaaatcat cttgcccctg
     5281 agcgtcatcg tctacgccgt ctacccggcg tgggtcgcgc gccttgcgct ccccttcccc
     5341 caactcgtgc gcctgctcgg cgttggcctc tgcctcgtct cgctcgtcca actcgtgcgc
     5401 gtccatcgcg cgctcggccg ccactggtcg gccagcctgc gcctgcgcga cgaccaccgg
     5461 ctcgtcaccg aggggcctta ccggcgcgtg cgccatccga tgtataccgc gctcatcggc
     5521 aacatgttgg gtctctgcct catgtcggcc aacctcttga ttatcgtgcc gcgcctcgtc
     5581 cagattttcc tcctcctgct acgcatgggg aaggaggagt cgatgatgct ctcgcgcttc
     5641 ggcgaagagt accgctccta catggcccgc tcgggccgcc tgttcccgcc gccgcacgcc
     5701 cgctgaaaac ggggcatgcc gcgcgaggcc cgacgccgcg ccctctgtca gtcagtttgt
     5761 tcagccgcgg ttcagcccgc gtatagacaa cgggcacgcc gttcgactat attttcgctc
     5821 tcttaaacgc attacacgca ttccatcagc ggagacaaaa acgcgtatgg cccgcttcag
     5881 cttcctgccg aaagaggagc agtactttgc cctcctcaag cagatgacct cctacatcta
     5941 cgacgcggcc cacggcctca cggaactgat ggccgaccag accggcgact acaccaaaca
     6001 cgcccgccac atcaagacga ttgaagactc ctgcgacgac ctgatccact ccatcgtcac
     6061 caagctcaac aagtcgttca tcacgccctt cgaccgcgag gacatctaca cgatggccgg
     6121 cgcgctcgac gacatcgttg acctgatcga cgactcggcc cgcgccatgg tcatcttcaa
     6181 cgtcagagag agcacggagc acgcgcgcga gttcgccgac gtgatcctgc gtatggccgt
     6241 ccagctccac gaggccatct cggcgctgga gcgccccgcc gacatcggcc cgcgcctcgt
     6301 cgagctgcac cgcctggaga acgagggcga cgacatctac cacaccgcca tcgccgaact
     6361 gttccgcgac acggccgacc cgctcaccgt catcaagtgg aaagagattt acgagaagct
     6421 cgaatcggcc gttgaccgct gcgagtcggt cgccaacatc atcgagagca tcatcatcaa
     6481 gcacgcgtga aaggagaggg atgagggcgg agggatgagg gatgaataag actgagtgaa
     6541 ggcgtcagtt gagcacccac tcaatccggt tcatccctca tccctcatcc ctcatccctt
     6601 ctatcatgga tccagccctc gtcttcgtca tcatcatcgt cgtcgtcgcg ctcgtcttcg
     6661 actatatcaa cgggttccac gacgcggcca actcgattgc cacggtcgtc tcgacgcggg
     6721 tgctctcgcc cggcgcggcc gtggtgtggg ccgccttctt caacttcatc gccttcctcg
     6781 tgctgggcga ggccgtcgcc aagaccatcg ggaaggacat gatcgacatc aaggccatcc
     6841 ccggtgactg gcagttgtgg gtcatcctgt gcggcctcat cggggccatc gtctggaacc
     6901 tcatcacgtg gtactacggc atcccttcga gttcgtcgca cgcgctcctc ggcggctacg
     6961 cgggcgcggc cgtggccgcc tcgggcctcg gcgtgatttt gccgaacggt tggacgaaga
     7021 cgctcatctt catcattctc tcgccgctca tcggcgcgct cctcggcttc atcatcatgg
     7081 tcgccgtgtc gtggattttc cgacgctggt cggcccacgg cgtggacaag ttcttccgca
     7141 aggggcaact cgcttcggcc gccctctaca gtctcggcca cggcggcaac gacgcgcaga
     7201 agacgatggg catcatcgcc ggcgtgctcg tcgcctcgaa cctcgactcg accgccacca
     7261 cggacatccc tctctgggtc gtgctcatct gccacgcggc catcggactg gggacgctct
     7321 cgggcggctg gcgcatcgtc cacacgctcg gctcgcgcat caccaagctc aagccggtcg
     7381 gcggcttcgc cgccgagacg gccgcggcga tgtcgctcat cggcgcgacc tacggcggca
     7441 tccccgtctc gacgacgcac accatcaccg gcgccatcgt cggcgtcggc gcgacccgtc
     7501 gcctgtcggc cgtcaagtgg ggcgtggcgg gccgcatcgt ctgggcgtgg gtgctgacca
     7561 tccccgcggc ggctggcatc gcggccctct cgttcctcgc ctatcgcctc ctcgccccgt
     7621 acttcgcctg acgcggccag ttccccggcc ttgcctcccg gacgcgcacg cgccggggca
     7681 cacgtgttgc catctggcgt cggggccgcg aaaactcgcg cattgcgcca cagaaacgta
     7741 cacaggggga ggctttagag aatgatgtca gaacagaagc gggccggctg gatcgtcccc
     7801 gaaaatcaga cttcgggtca ccatacggac tcgtcgaaga ccaacccgac gcggccgggc
     7861 gcgggcgaag aacatttcga ggaaggccgc tcgcagcccg gcgtcgccgg tcagggcgcc
     7921 gaccaactga gcgacgccga ccgcgcaagg ctaaaagacg tgaatcgtaa atcgtaaatc
     7981 gtgaatcgat tgtcgctcgg tcgaagttgg tttctttgac aaagtcagtc ggctctcaat
     8041 ttcaatgtac gattcatgct cgacgcgttt ttttacgatt gacgattgac gagaaggagg
     8101 agccgtgcgc tggagagatc gagctggaag tgcgaacgtt gaggacaggc gcgggatggg
     8161 ccgcggcggg atggctgtgg gcggcggcat cggcacgctc gccatcgtgt tgctggcggc
     8221 cttctgcggc gtagacccga gcgcgctcct gaatcagttg ccgcaggaag gcggcggggc
     8281 gccggggccg caggccgagc gccgcgtcaa tcccgaggag gatgagctag cgcagttcac
     8341 gagggtcgtg ctgcgcgaca cggaggagac gtggaacgag gtcttccggc aggcgggccg
     8401 gcagtatcgc gagccgacgc tcgtcctctt cagcgggcag gtgcagtcgg cgtgcggtct
     8461 ggccggcgcg gccgtcggcc cgttctactg ccccggcgac cagaagctct acatcgacct
     8521 ctccttcttc cgcgaactgc gggcacgctt ccgcgcgccc ggcgacttcg cccaagccta
     8581 cgtcgtcgcg cacgaggtcg gtcatcacat ccagcacctt ctgggcacga tggacaaggt
     8641 acacgccgcg caacagcgga tgcgcggggc cgaggccaac cagctctccg tgcgtctcga
     8701 acttcaggcg gacttctacg ccggcgtgtt cgcgcatcac gtccagcgca agggctacct
     8761 cgaagctggc gacgtggagg aggcgctcaa cgccgcgacc gccatcggcg acgacaagct
     8821 ccagaaggag ggtcagggct acgtcgtccc cgactccttc acccacggca gctccgagca
     8881 acgcatgcgc tggttccgca agggccttga gaccggcgac atccgacagg gcgacacctt
     8941 cagcacgcgg agtctataga gcgagagagc gagcgccgcg tcagcacagg cgacaacgag
     9001 ccgcggagcg gcggcagaat gtagccacgg gcgctgggcc cgtggaacca gcaacaaaca
     9061 gcaacaagcc ccggaggggc gaaagaatcg tgggcggtgt ttcacgccgg ttgcgattct
     9121 ttcgcccctc cggggctttg atcctttaat cgaactcgtc cccacggctc acgccgtggg
     9181 ctacattctg ccgccgctcc gcggctcgtt gtcgctcagc ctttaattgt tcctgccctc
     9241 cgtcggcgtc tcgccgcgcc gctcccagaa gaagggcgtg cgcttcctct cgacgggata
     9301 gacctcgtcc atgttgcgcg tgtcgtagag cacgccgccg atcatcacgt gcgtgatcga
     9361 ctccgagtga cgcaaattct ccagcggatt cttgtcgagc acgatgaggt cggcgagctt
     9421 gcccggctca agggagccga ggtcggcgtc gaggccgagg taggccgcgc cgtggagcgt
     9481 ggcggcgcgg agagattcga gcggggtgag tccgccctgc ccgagcatcc acagttccca
     9541 gtggacgccg agtccctgaa gctgcccgtg cgccccgagc tgcgcgcggc ccccggcgcg
     9601 caccacgtcc ttcaccgact tcgacagctc gaagtggaag aagtcgtctt cgggcgccat
     9661 catgcggcgg cgcgagcgct cgtcgatgat gcggcggggc gtgaagcgca ggagcttttc
     9721 gttctcccag acgttcgagt gctgatacca gtagttctca ccccagacgc cgccgtagcc
     9781 gacgatgagt gtgggcgtgt agccggcgcc gctgcgggct aagagcgtca cggcgtcctt
     9841 gtagagcggt gcgacgggga tggcgtgctc gatgccggtg tggccgtcga ggacgtggcc
     9901 catgttgaag tagtaggtgg agccgccctc ggggacgacc atcatctgga gttcgcgcgc
     9961 ggccttgatg atgcgctggc gctggtcgcg ccgtggctgg ttgtagctct tgacggagaa
    10021 ggcgcccacg gccttgaggc gcgcgagatg cgagcgcgcg tcctcgtaag agttgacgac
    10081 ggccttctcg gggttctccg cgccgtagag gatgtagccg gtggagaaga cgcgcggccc
    10141 cgtcatcgcg cccgacttga ccatctccga ctgtgagaag acgagctgcg tggaggccga
    10201 cgggtcgtgc gtcgtcgtca cgccgtaggc gaggttgacc cagtagggcc actgccgctc
    10261 ggggatgatg tcgagcgtgt tgtagcccat gtgcgcgtgt acgtccacga agccgggcat
    10321 gacggtcttg ccgcgcatgt cgaagacgcg cgcgcccgcc gggacggcga cgcgcgcgcc
    10381 gaccgaaacg atgcggtcgt ctttgatgac gaccgtgccg cgtgcgatca cctcgtcgcc
    10441 gcgcatggtg atgacgcgcg cgttcgtcag cgccaccgtg ccgcgcgggc gcgccgtctc
    10501 aaactcgaag ccgatgcgcg tggcgcgcgg ctcgggcgtc ttctcgtctt tggcgagctg
    10561 cttctggatg ttcgtgaggc tctgctcgta gaagttttcg ccgagcgtcc actggacgga
    10621 tgtgccgtcg ggcgaccaag tgagccagtc gcccgactcc ttcgagatgg gcttgacggg
    10681 gacggtcgcc tccgtcgatg agagcttgag agtcttgccg gccttcggga acggcgcgac
    10741 gtagagcttg tgcagctcct tgaaggcgac ccactcgtgg tcgggcgagg ggatgatttc
    10801 ggtgacgtag ttggactcga tgtgccggcg ggagtcgtcg ccggccagct tgacgctcga
    10861 cagatacgtg atgaactgcg cgggctgtgt cggcgcgccg ccgccgcccg agccggcttc
    10921 catgaagaag atgcgctcgc ccgccgggtc gaaactcaac acgggcatgc gcgcgttggg
    10981 gccgcggctc tggaggtcca tcacgtagtt gtgcttgccc gccgcccagt agtgaatctc
    11041 gaactgcgac tcgctcgcca agtccgcttc gtggtagatg ctgccacgcc ccttgagata
    11101 ggcgatcttc gtgccgtcct gcgagaagac ggggttggcg tactggtcgg ccgtggtcgt
    11161 gatcttcgtc tggttcgacc cgtcggcgtt cgccacccag accgcgccct tctcctcgtc
    11221 gctccacgtg acgtaagtaa tcatcagccc gtcagggcga aagctcggcg cgtactcgaa
    11281 gtacttcgag ttgagcaggc gtcgcggctc ctgtgcgccc tccttcacgt aaatcttgcc
    11341 gagcgcgctg tagacgacgc gctcccccgt gctccgcgcc cagcgcagca gacgcgccgt
    11401 gtcgcgctcg ggcgcaacct tctgccggaa gcgcaccggc tccgagacct tctgcttgac
    11461 gtgcgccgtg aacgggatgg gcgtcactgc cctcgtgttc acgtcgatgc ggacgatctt
    11521 gcctttggcg tagatgacga ttgacttgcc gtcgggcgtc caactgtagc cggggtaggt
    11581 gccgaagagc gcccacgtct cctgctgatc gcgcgtgagg ccgtcgaaga cgggccactc
    11641 gcgcccggtc tcgacttcac gcagatggag cacggacttc aagccgacgc ggcggatgaa
    11701 ggcgaggagt ttgccgtcgg gcgaaatctg cgggcgcacg gagccgccgg agccgcggac
    11761 gaaggtggag cggcggccct tctggagatc gtagcggtcg atccagtaga tgttctcgta
    11821 gacgttcttg ttgtagtcga aggcattgga ggcgacgaag tagacgaagc ggccggtggg
    11881 gtcaacttcc ggctcgccga tgttcgccgt ccagttcggc ttctcagtaa tctgcacgcc
    11941 cttgccgccg ccctgaatgt ggtacatcca gacttcgccc gcgccggccg agcgcgtgtc
    12001 ggtgaggtgc ttgcgcgcga acaggtaatg cccgtcgggc gaccacgccg gcgtgctcat
    12061 ctgccgcgtg gtctccttcg tcacctgccg gcggtttttt ccgtcggcgt ccatcaccca
    12121 gatgttttcg acgccatcgc ggtcggaggt gaaagcaacg agcttgccgt cgggactgaa
    12181 acgcggctgc gcctcgaagg agacgccgct cgcgacgagc tcggcgcgtc cgccggcggc
    12241 cggcatgcga tagatgtcgc cgaggaggtc gaaggccaac cactgtccgt cgggcgacac
    12301 gtcaacgctc atccaagtgc cttcgtccgt gtcgaactcg actatgcgcg aggggccgtg
    12361 gtcgccctcc acgtcccacg gctcattctt cttcgcttcc ggcgcggtgt ccggcttctt
    12421 ctgcgaatcc tgtacggcgc cgggcttgtt cgccggctcc tgcgcgctca cgccctgcgc
    12481 gccgaaaacg acgagaaagg ttaagagaca gacggacaat cttctgagca cgttttcacc
    12541 tctgaaaagc agtgataagt gatgagtgat aagtcataag aaaaactgtc tgctctttct
    12601 tatcacttat cactcatcac tcatcactcg tcaaatggtc tgcgacgctg taagccgggc
    12661 cgcagacgag cagaatcgtc atcggctccg cgcccgtgtt ttcgagcgtg tgcgcggagc
    12721 cgcgcgggat gtaaaccgcg tcgcccggcc cgacctcgcg cgtctctgcg ccgaccgtca
    12781 tgcgtccccg cccggccgtg atgtagtaga cctcttccgt cgccgtgtgg aagtgccggc
    12841 cgacggccgc gcccggcggc agcgtctcct cggccagact gcacagctcc acacgggagg
    12901 tcgtgcggtc gatgagcggt cgtatctcgg agccgtgcgg cgtgttgatg acggcggcct
    12961 gccggcgatt tacgacaatc atagatggtc gcctgagttc ggatgctctg cggtgttttg
    13021 gggtatcgcg ttgtgacgaa cggataaaga tttacgcggc gaagcgcgac cctgtcaagc
    13081 ccgtggggtg gctggcgcga atcggcgcag tcatgctatt ttttctccca cgcccgcgta
    13141 gctcagtgga tagagcgctt gcctccggag caagaggtcg caggttcgac ccctgccgcg
    13201 ggtatcaatc gacgaggccg gccgcgaggc tggctttttt ctttcacgct ggcaccccgc
    13261 ttgcccccac acccccagag actcttctgc gcgttcggga agcgtgcaga tttcttgtac
    13321 acgaagacag aataggacaa ggagttttcg atgaaggttt tcgacatcct gagacaggat
    13381 caccagaagg tcgccgacat cttccaacag ttggagccgg ccgccgacga cagcacacgc
    13441 cagcagctct tcacacaact caagcaggaa ctcgacctgc acgcgcacgt cgaggagacg
    13501 atcctctacc ccgcgctcaa ggacgcctct cagacgcgcg agatcatcct ggaggcctac
    13561 gaggagcacg ccgaggtcaa ggagctgctc gcgcgcatcg agcagacgcc gccggccgac
    13621 gcgaagtggg ctgacctctt ggccgagctg cgccacaacg tcgagcacca cgtggacgag
    13681 gaggagagcg agatgttcga cgcggcgcgc gatgttctga gcggcgcgca gatcgacgac
    13741 atcacgagac gtgtggcgga ggccaagcag cagggcctga aggccgcgga cgcgggcagc
    13801 ctctaaagtc tcttgagcat cgacaaccct ttcgcgggtg gggtgagccg cttcgtcccc
    13861 cacccgcgcc tgtttgcgtc tttttcagtg gcaaacaggc gcgcgcggtt tatcatcaaa
    13921 gacaaggcgg cacttcaagc tcccactgac cttgcagtaa agtaattttt tattggagga
    13981 atcatgcaca gattcagact gaagggcttg acccttgccc tgttaatgct gctgccggcc
    14041 gtgccgctcc cgcccgtgcg cgcgcagcag cagcagcagg cccagagcgc cgccgccgcg
    14101 acgccggccg cggcccaggc aaagcgcctc gcgctcacca cctcttcggc cgcgcgcaag
    14161 gcggccgagc gcatcaccgc cgcgcagatg aaggaagacc tctactgggt cgccgacgac
    14221 gcgcgcggcg ggcgcgacac cccttcggcc ggtctggacg cgaccgccaa gttcatcgcc
    14281 gaccgcctcg ccaaagccag gatcaagccc gccggcgacg acggcaccta cttccagaaa
    14341 atcgaactcc gcagcacgag cgtggactcc gagaagacgc gcgtccaact cgcgggccgc
    14401 gccttcaagc tcggcgacga tttcgtcatc gccagcacgg cgagcgccga ggcttcgggg
    14461 ccgctcgtct acgtcggcca cggctgggtc gtccgctcga agaacatcaa cgcctacgaa
    14521 ggtctcgacg ttaagaataa gattgtgatc gtcgcgggcg ctggcgtcgc cccgcccgag
    14581 ggcgtcaccc ccgaatacat cgcgggccgg ccggcctccg actgggaaag ccccctcagc
    14641 tacgccgaga agaacggcgc acgcggcgtc gtcttcgtcc cgaaaaatca cgaacgcatc
    14701 tatcagtttt accgccgctc gcttggccgc tccagttatc aagtcaagcg gttcgtcgag
    14761 gagtccgagg gtcgggccgg cgccccgccg agcaacatcc ccgtcatcat ccccacggcc
    14821 gagatgctca ccgcgctctt cgagggcgag cgcaccgcgg gcgccgacat catgaaagcg
    14881 gccaacgcaa acgcgggcga tcagggattt gacttccccg ccgccaagac gatgagcatc
    14941 gcgacctccg ttaaggtgtc ggaggcgacg acgcagaacg tcgtcggcgt gattgaaggc
    15001 tccgacgcga aactccgtga ggagtacgtc gccgtcggcg cgcactacga ccacgtcggg
    15061 atgcgtcccg gccagagcgg cgacaacatc ttcaacggcg ccgatgacga cggctccggc
    15121 accgtcgccg tgctctcgat ggccgaggcg ttcgctaagg ccaggtcgcc caaacgctcc
    15181 gtcctatttg tctggcacgc cggcgaggag aagggtctgt ggggctccca gtacttcacg
    15241 aagttcccca cggtgcccat caagcagatc gtggcgcagc tcaacatcga catgatcggc
    15301 cgcagccgcg cccccgacga catcaaaccg gccaacaagg gcttgagtgg gccggacgag
    15361 attttcgtca tcggctcgaa gatgatgagc gccgaactcg gcgaggcgag cgaagccgtc
    15421 aaccgctcct atctcaacct caagtacaac tatcaatacg acgcgcccga cgaccccgaa
    15481 cagctcttct atcgcagcga ccactacaac tacgccgttc agggcatccc catcgtcttc
    15541 tacttcgacg gcatccacga ggactatcac aaggtcacgg acacgcccga caagatcgac
    15601 taccagaaga tgcagaaggt cacgcgcacg gtcttcgtcc tcgccaccga actcgccaac
    15661 gcgcccaccc ggccggtcgt agacacgccc ctgccggtca gcagccggcg gcgctgactt
    15721 ctttcattag cggcgtgggg ag
//
LOCUS       DASVES010000278        13968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_786229, whole genome shotgun sequence.
ACCESSION   DASVES010000278 DASVES010000000
VERSION     DASVES010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13968
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786229"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VF240_11180"
     CDS             complement(<1..1322)
                     /locus_tag="VF240_11180"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7175812.1"
                     /translation="MSVAPRKKKKRELPFEQRVFLFAVLAGLPGSFVALTMLWRGPYT
                     PKVQWTLSVLIVSLWWGLAFTLRERVLRHLQTLSNLLAALREGDYSIRARGARHEDAL
                     GEVMIEVNTLGETLRQQRLAELEASALLRTVMSEINVAVFAFDGGNRLRLVNRTGERL
                     LAVAAERLLGRSADELHLADYLRGEDASTVTRVFPGAAGRWGVRRTAFREMGQQHHLL
                     VISDLSRPLREEELEAWQRLVRVLGHELNNSLAPIKSIAGSLETLLVREPKPDDWRED
                     MRRGLTVIGARSAALSRFTRAYARLAKLPQPRFEPVDVGSWVRRVVELETRTHVALTP
                     GPAAVIEADEDQLDQLLINLLRNAVDASLQTGGGVRVWWARNATHLSVVIEDEGPGLS
                     NTSNLFVPFFTTKPEGSGIGLVLSRQIAEAHGGTLTLQNRIGIRGCVAR"
     gene            complement(1319..2704)
                     /locus_tag="VF240_11185"
     CDS             complement(1319..2704)
                     /locus_tag="VF240_11185"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7175813.1"
                     /translation="MKTDSNAPRVLVADDQPDVLEALRLLLKGEGYRTELSSSPALAL
                     AALEKRDFDLALIDLNYARDTTSGEEGLELLSRLQTVDSSLPVVVMTAWGSVEVAVEA
                     MRRGACDFIQKPWDNTRLLTVLRTQVELGRAVRKGLRLEEENRMLRDDGNGSAGGRPT
                     FIAESPQMRPVFQLIESVAPSDANVLITGENGTGKEVVAHTLHFLSERAAKPMITVNV
                     GALSEGTFESELFGHVRGAFTDARADRVGRFELADGGTLFMDEIANIPVSQQAKLLRV
                     VETGEFERVGSSKTRRVDVRILSATNADLKEEVSAGRFRQDLLFRLNTVEIRLPPLRE
                     RREDIPVLARYFLARHARRYRKPAADFDSAAAAALAAHAWPGNVRELNHTVERAVLMS
                     KGAAVSAGDLGLNGEAGGSRSLEEMSIDEVESYLIKKTLARFDGNVGQAAEALGLSRS
                     ALYRRLQRYGL"
     gene            complement(2932..4182)
                     /locus_tag="VF240_11190"
     CDS             complement(2932..4182)
                     /locus_tag="VF240_11190"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7175814.1"
                     /translation="MRKLFVFVAALAFAALLGAGGVMQARQERGAREKRVRERSAEQV
                     APVDVREELEGLRSPDAQKRAAAAYRLKQRGAAARAAIPALVELLGDATVVDPSLYRK
                     GEKWWNNRTDITVGVEASHALSEIGAASVEPLIGALRRPEAEARKNAAWALGAIDDKR
                     AVEPLIGTLKTDADARVRSTSAWSLGAIGDARAVEGLNAALKDADAEVRKQSAWASGA
                     IGDERSTSSLTQALKDDDAEVREQAAWALGAIGEGGPVVNSLIAALGDTSKDVREQVS
                     WALGAIGDKRAAAPLAAALRSLNGDVLEQAAWALGAIGDEDATEALSEVVSGPGMAQG
                     AGARSQAAWALGAIGDRRGVPSLIGALKDESASVRSQAAWALGAVGDRRAADALKAVL
                     SDKSKDVRSQAAWALSVIGELGKN"
     gene            complement(4603..7050)
                     /locus_tag="VF240_11195"
     CDS             complement(4603..7050)
                     /locus_tag="VF240_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7175815.1"
                     /translation="MGTFFQDVRFGWRRLKASPGFTAVAVLSLALGIGANTAIFTLVN
                     TVLLRPLPVAEPERLASVYPVARDEEVGAFSYPDYRDFRDRNEVFTGLFVHRFAPMSL
                     SREGQSERVWGYLVSGNYFDVLGVSAARGRTFLPEEDVAPLRHPVVVVSHASWQTRFG
                     GDPNLIGKTVQLNGHPFSVVGVAPEGFQGSETAYTPELWAPMMMQPVIEPGFDWLEKR
                     DNHNLFATGRLKPGVTYEQAVASLNILARQLGEEYPDTNEGMTMTLTPPGLIHPILRG
                     PVIGFTGALMGVVALVLLIACTNLANLLLARAADRRKEIAIRLALGASRLRLVRQLLT
                     ESVLLALMGGAAGWLLALWVVDVVTAFKPPVNFPLTIDLQIDWRVFAFSLVASVLTGI
                     VFGIVPALQTTNPELVPALKDGAAQGGARRTRLRSALVVAQVALSLVLLVAAGLVVRT
                     LQHVQTMSPGFDPEGALTMSVDVGLQGYDEARGRQFYRQLLERLRALPGVESATAADF
                     LPLSLNYNAMPVFIEGQPAERGANVPTAMNSSVATDYLKTMGIPLVRGRDFGERDRED
                     APRVVVVNEAFARRYFPGADPVAEAIGHRVSSDAEGPWYEIVGVAGDGKYFGISEAPR
                     PFIYLSLGQRYSSNASLVVRTKGDPRAMIAAVRGEVRGLDESLPVFDVKTMTEHLGIS
                     LFPARVAGALLGAFGLLALTLAAVGIYGVMSYSVAQRTHEIGVRVALGARPRDVLGLI
                     VRQGMTLAVVGIVLGLLAAFAAVRALGNLLYGVGATDPLTFAGVTLILASVALAACLI
                     PARRATKVDPMVALRYE"
     gene            complement(7164..9596)
                     /locus_tag="VF240_11200"
     CDS             complement(7164..9596)
                     /locus_tag="VF240_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7175816.1"
                     /translation="MTTFMQDLRYGARMMWKSPGFTAVAVLALALGVGANTAIFSVVH
                     ALMLRELPYRDSERLVMVWEANRPRDRHQNVISPANFIDWGERNTVFEDMAAFVDYRS
                     NLTGMGDPVEVPVQGATPNMFSLLGVEPILGRGFTAEDAAQNAPPVVVLSYPFWQSRF
                     GGDPSVVGKTMTLGGEAATVVGVMPAGFQWLIQKGSLTGKQPEVWEPFGLGEQHRIRR
                     GRYMMSVARLKPGVTRERAQAEMDSVAAQLEGEYADFNKGWSVEVVPLRQQFAGEIST
                     ALWVLLGAVAFLLLIACANVANLLLARAAARQREIAIRRALGAGRWRVVRQLLTESVL
                     LALVGGALGVLLAWWGVETLARLSPRDLTDLGAVELNLPVLGFTLAVSVLTGIIFGLA
                     PAFEATRLNNNEALKEGSRGSTGGARSRRLRAAFVVVETALALIMLVGAGLLVRSFAR
                     LRSVDPGFRAANVLSMQVPLPRKYAEPQARINFFKEAVARIGAVPGVESAGAISFLPF
                     AGLGAATRFTIVGQPPPPPGEDLTTEVRVTDTNYFRTMDIPVTRGRTFNEQEATEIRR
                     VVVVNEAMVRKHFPGEDPLGKRLVVSMSNEPQPTEIIGVVGDVRLNNLTAEVRPTVYW
                     PHPELAYSSMTIVARTTADPAAITAAARREIQSIDPDQPISDVRTMEELLSASVARAR
                     FSTTLLGVFAAVALLLAVVGIYAVMSYTVTQRTHEIGIRMALGAQGRDVLGMVVGHGL
                     LLALAGVVLGLLGAFALTRLMSSLLFGVTATDPLTFSAVALLLAAVALLACYLPARRA
                     TKVDPMVALRYE"
     gene            complement(9713..12172)
                     /locus_tag="VF240_11205"
     CDS             complement(9713..12172)
                     /locus_tag="VF240_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7175817.1"
                     /translation="MRTFLQDLRYGLRVLRKSPGFTAVAVLTLGLGIGVNTAIFSGVS
                     AFMLRPLAGVSDAERLVALFEATDGSAYDDVSYPDYLDYREQSTTLDGIVGHQMVRAA
                     VGDETQSEVVWGQLVSADLFDVLGVRMRMGRGFLPEEERTPGTHPVIVLGPHLWRNRF
                     AADAGIVGRTVSVNGRPFTVVGVAPDDFAGAKWALRMDFFVPLGMQEQVMGGQSWLND
                     RDSHWLSLMGRLKPGATREQAAAELSTIARRLNEAYPNERTKGARVNVVTEQDSRFQD
                     MAGVARMGGGLALAIVGLVLLIACANVANLLLARSVSRRREIAVRLALGAGRARIVRQ
                     LLTESLLLAAAGGLTGLLLSFWLTDMMTGFFPALPYAMSFSVAPDARALIFTLAVSLL
                     TGLAFGLAPALQASRPNLIPVLKGETPQARGRGRRFSMRNLLVVSQMALSLVVLVCAG
                     LFVKSFNNAKTVDIGFTSEGVMTASLNPGLFGYTREQGREFYRQLIERVEALPGVEGA
                     GAAYLLPLGDSSTSWGPVYAAEQPVPPPGEGRGVQINIVSPGYFDTLQIPLLRGRDFN
                     AHDLPGAKPEAVVINETFAARYWPGEDPLGKRVKVGRDDPETLEVVGVVKDGKYRTLG
                     EPPRPFMFVSLDQTYQSGMTLVVRAQGDPARYVEALREATKSVDARVPLYNVKTMREH
                     LTWALWAPNMAASLAAAFGILALLLAATGLYSVLAYAVSQRTHEIGIRMALGAQRGDI
                     LRLITRQGMALALVGLVLGLGAAAGSARVIASLLFGVSPTDAYVFAAISLALALVALL
                     ACYIPARRATKVDPMVALRYE"
     gene            complement(12262..>13968)
                     /locus_tag="VF240_11210"
     CDS             complement(12262..>13968)
                     /locus_tag="VF240_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7175818.1"
                     /translation="VAAISNRLEAAYPAENRGRVARLVGLQEMLVEDVRPALLILLAA
                     VGFILLIGCANIANLLLARGASRVKEVALRAALGASRWRLMRQFITESVLISLAGGVL
                     GALLALWGVDLLMSLNPGELPGGQSPRLDARVLLFTLGVSLLTGVVFGLMPALHLSKP
                     DLNESLKEGRTTGTGGARGRLRSIFIVSEVALSLVLLVGAGLLVNSFYRLLNTSPGFA
                     PEKLLTLEYRLPRNKYPELAQQWDFHRRMVERVREVPGVEAASVIRAIPFSGNGSSAT
                     FVLPDRPAPAPGGEPRALTNTAAPGYFETMRLPLSTGRTFDERDKADSPTVFIVNQMF
                     AKQHWPGEDPIGKRIEVPADKMSGVVVGVVGDAKQFQLNEVQQPQIYYAYAQAPFIFA
                     SLVARTKVEPMSLADDVRAAVWSIDKDQPVWKVRTMETLLTTNVASPRFVMTLMTGFA
                     ALALLLTALGLYGVMAYVVSQRTHEIGIRMALGAQRGDVLRLVLRQGMVLALIGVGLG
                     LAAALAATRAMQSLLFGVTANDPLTFAAVSLVLTLVALLACYVPARRATKVDPMVALR
                     YE"
BASE COUNT         2289 a         4755 c         4828 g         2096 t
ORIGIN      
        1 cgcgccacgc agccgcggat gccgatgcgg ttctggaggg tgagggtgcc gccgtgggcc
       61 tcggcgatct ggcggctgag gacgaggccg atccccgacc cttcgggctt cgtggtgaag
      121 aagggcacga agaggttgct cgtgttcgac agacccggcc cctcgtcctc gatgacgacc
      181 gacaggtgcg tggcgttcct cgcccaccag acgcgcacgc cgccgcccgt ctgaagcgac
      241 gcatcgacgg cgttacgcag gaggttgatg aggagctggt cgagttgatc ctcatcagcc
      301 tcgatgacgg ccgccggccc cggcgtgagc gcgacgtggg tgcgcgtctc caattccacg
      361 acgcgccgca cccaactgcc cacgtccacc ggctcgaagc gcggctgcgg gagcttggcg
      421 aggcgggcgt aggcgcgcgt gaagcgcgac agcgccgccg aacgcgcgcc gatgacggtg
      481 aggccgcggc gcatgtcctc gcgccagtcg tcgggcttcg gctcgcgcac gagcagcgtc
      541 tccagacttc cggcgattga cttgatgggc gcgagcgagt tgttgagttc gtggccgagc
      601 acgcgcacga gccgctgcca cgcctccagc tcctcctcgc gcaacgggcg gctgaggtcg
      661 gagatgacga gcaggtggtg ctgctggccc atctcgcgga aggcggtgcg gcgcacgccc
      721 cagcgtccgg ccgcgccggg gaagacgcgc gtgacggtgc tcgcgtcctc gccgcgcagg
      781 tagtcggcga gatggagttc gtcggcgctg cgtccgagca gacgctcggc ggccacggcg
      841 aggaggcgct cgcccgtgcg gttgacgagc cgcaggcggt tcccgccatc gaaggcgaag
      901 acggcgacgt tgatctccga catcacggtg cgcagcaggg ccgaggcttc gagttcggcg
      961 aggcgttgct ggcgcagggt ctcgccgagg gtgttgacct cgatcatcac ttcgccgagg
     1021 gcgtcttcgt ggcgtgcgcc gcgggcgcgg atggagtagt cgccctcgcg cagggcggcg
     1081 aggaggttgg agagcgtctg gaggtgtcgg agcacgcgct cgcgcagggt gaaggccagc
     1141 ccccaccaga gcgagacgat gaggacgctc aaagtccact ggactttggg ggtgtagggg
     1201 ccgcgccaga gcatcgtgag cgcgacgaaa ctgccgggca gccccgccag gacggcgaag
     1261 aggaagacgc gctgctcgaa gggaagctct cttttctttt ttttgcgcgg ggcgacactc
     1321 ataggccgta acgctggagg cgccggtaga gcgcgctccg gctcaggccc agtgcctccg
     1381 cggcctgccc gacgttgccg tcgaagcggg ccagagtctt cttgatcagg taagactcta
     1441 cctcatcaat gctcatctct tcgagcgagc gactcccgcc ggcctcgccg ttgagtccca
     1501 aatcgcccgc cgacacggcc gcccccttcg acatcagcac cgcgcgctcg accgtgtggt
     1561 tgagttcgcg gacgttgccc ggccaggcgt gggcggcgag tgcggccgcc gccgcactgt
     1621 cgaagtcggc ggccggcttg cggtagcggc gcgcgtggcg cgcgaggaag tagcgcgcca
     1681 gcacggggat gtcttcgcgg cgctcgcgca aagggggcag tctgatctcc actgtgttga
     1741 gacgaaagag caagtcctgc cggaagcgtc cggcggacac ttcttccttg aggtcggcgt
     1801 tggtggccga gagtatgcga acgtccacgc gccgcgtctt cgacgagccg acgcgctcga
     1861 actcgcccgt ctcgacgact ctcagcagct tggcctgttg cgagacgggg atgttggcga
     1921 tctcgtccat gaagagcgtg ccgccgtcgg ccagctcgaa gcggccgacg cggtcggcac
     1981 gggcgtctgt aaacgcgccg cgcacgtgtc cgaacagctc gctctcgaag gtgccctcgg
     2041 agagcgcgcc gacgttgacg gtaatcatcg gcttcgcggc gcgctcgctt aagaagtgaa
     2101 gcgtgtgggc gacgacctcc ttgccggtgc cgttctcgcc cgtgatgagg acgttggcgt
     2161 ccgagggggc gaccgactcg atgagctgga agacggggcg catctgcggc gactcggcga
     2221 tgaaggtggg acgccccccg gcgctgccgt tgccgtcgtc gcgcagcatc cggttctctt
     2281 cttcgagccg cagccccttg cggacggcgc ggccgagttc gacctgcgtg cgcagcaccg
     2341 tgagcagacg cgtgttgtcc cacggcttct gaatgaagtc gcacgcgccg cgccgcatcg
     2401 cctcgacggc cacctcgacg ctgccccacg ccgtcatcac gacgacgggc agactggaat
     2461 cgaccgtctg gagccgcgac aaaagctcca gcccctcctc gcccgaggtg gtgtcgcgcg
     2521 cgtagttgag gtcgatgagc gcgaggtcga agtcgcgctt ctccagcgcc gccagcgcca
     2581 gcgccggcga actcgatagc tccgtcctgt agccctcgcc cttgagcaac agccgcagcg
     2641 cctccagcac gtccggctgg tcgtcggcca cgagcacccg aggggcgttt gaatcagtct
     2701 tcataaaagc agcgattagc gattagctgt tagctattag ctgtcaagtg tcggccagga
     2761 cagggcgaac cagagattca tttactgcga ccatcccgcc tctggctaat agcaaacagc
     2821 taatcgctaa cagctttatt catatcgaag cgcgacgacc cggtcaaccc gcgcggcgtg
     2881 ttgaagacgc gggcgacggc ggcgggccga cggcgcgacg cgcggggtcg gtcagttttt
     2941 gccaagctcg ccgatgacgc tgagcgccca cgccgcctga gaacgcacgt ccttgctctt
     3001 gtcgctcagc acggccttga gggcgtcggc ggcgcgtcgg tcgccgacgg cgccgagcgc
     3061 ccacgcggcc tgcgagcgaa cactcgcgct ctcgtccttg agcgcgccga taagcgaggg
     3121 gacgccgcgc cggtcgccaa tcgcgcccag cgcccacgcc gcctgagagc gcgcgcccgc
     3181 gccctgcgcc atgcccggcc cactcacgac ctcgctgaga gcctcggtcg cgtcctcgtc
     3241 gccgatggcc ccgagcgccc acgcggcctg ttccagcacg tcgccgttca gactcctgag
     3301 ggcggcggcc agaggcgcgg cggcgcgctt gtcgccgatg gctccgagcg cccaagacac
     3361 ctgctcgcgc acgtctttgc tcgtgtcgcc gagcgcggcg atgagtgaat tgacgacggg
     3421 gccgccctcg ccgatagcgc cgagcgccca tgcggcctgc tcgcgcactt cggcgtcgtc
     3481 gtccttaagc gcctgagtga gcgacgaggt ggagcgctcg tcgccgatgg cgcccgaggc
     3541 ccatgccgac tgcttgcgaa cttccgcgtc agcgtccttg agcgccgcgt tcagaccctc
     3601 gacggcgcgc gcgtcgccga tggcgccgag cgaccacgcc gaggtgctac gcacgcgcgc
     3661 gtccgcgtcc gtcttgagtg tgccgatgag cggctcgacg gcgcgcttgt cgtcaatggc
     3721 cccgagcgcc caggcggcgt tcttgcgcgc ctcggcttcg gggcggcgga gcgcgccgat
     3781 gagcggctcg accgacgccg cgccgatctc ggagagcgcg tgcgacgcct cgacgccgac
     3841 ggtgatgtcc gtgcggttgt tccaccactt ctcacccttg cgatacagac tcggatcgac
     3901 gacggtcgcg tcgcccagca actcgacgag cgctggtatg gccgcgcgcg ccgccgcgcc
     3961 gcgctgtttg agccggtagg cggcggccgc gcgcttctgc gcgtcgggcg agcgcaagcc
     4021 ctccagttcc tcgcgcacgt ccacgggcgc gacctgctcg gcagacctct ctctgacgcg
     4081 cttctcgcgc gcgccccgct cctgccgggc ctgcatcact ccgcccgctc ccaacagcgc
     4141 ggcgaacgcg agcgccgcga cgaagacgaa taactttctc aaacggacat caactctcat
     4201 ggaacctcct gcccttttgc catgcgtcgt aactcgggag acgtgtaggg gctggacttg
     4261 tccctcgcac gacgcggatt gattgcaacc tcgatgttta cgaattgacg tgggccggag
     4321 cagttgcgcc tccgccctca aagcgtgtct ggaaatccac gtctgggccg cggagcggct
     4381 gccagaaggt agcccgcacg tgcaacgtct gggacacgcc gccacgatgt ccccgcgctg
     4441 aaggcgcggc agagcgtccg cgcgcccctc cagggcgcgg acatgatttg gacgccgacc
     4501 agacgtttta cgtctggcta ccttctcgtc gccgcttcgc ggctgaacac aatttccaaa
     4561 catgctctca ctgctcactg cttactgctc actgcttact gctcactcgt atctcaaggc
     4621 caccatcggg tcaaccttcg tcgcgcgtcg cgccgggatg aggcaggccg cgagcgccac
     4681 cgacgccaga atcagcgtga cgccggcgaa ggtcagcggg tcggtggcgc cgacgccgta
     4741 gagcaaattc ccgagggcgc gcaccgcggc gaaggccgcc agcaggccca gcacgatgcc
     4801 gacgacggcg agcgtcatgc cctgtcggac gatgaggccg agcacgtcgc ggggccgcgc
     4861 gccgagcgcc acgcgcacgc cgatttcgtg cgtgcgttgc gccaccgagt aggacatcac
     4921 cccgtagatg ccgacggccg cgagcgtcag ggccagcagc ccgaaagcgc ccaacagcgc
     4981 cccggccacg cgcgccggga agagcgaaat ccccaggtgc tcagtcatcg tcttcacgtc
     5041 gaagacgggc aggctctcgt caagcccacg cacctcgccg cgcacggccg cgatcatcgc
     5101 gcgcgggtcg cccttcgtcc gcacgacgag gctcgcgttc gacgagtagc gttgccccag
     5161 agagaggtag atgaacggcc gcggggcctc ggagatgccg aagtacttgc cgtctccggc
     5221 cacgccgacg atctcgtacc acgggccttc ggcgtcgctg ctcacgcgat ggccgatggc
     5281 ctcggccacg gggtcggcgc cggggaagta gcgccgcgcg aaggcttcgt tgacgacgac
     5341 gacgcgcggc gcgtcctcgc ggtcgcgctc gccgaagtcg cgcccgcgca ccagcgggat
     5401 gcccatcgtc ttgaggtagt cggtggcgac cgacgagttc atcgccgtcg gcacgttggc
     5461 gccgcgctcc gccggttgcc cctcgatgaa gacgggcatc gcattgtagt tgagactcag
     5521 cggaaggaag tcggcggccg tggccgactc gacgccgggc agggcgcgca ggcgctccag
     5581 gagctgtcgg tagaactgcc ggccgcgcgc ctcgtcgtag ccttgcagac ccacgtccac
     5641 cgacatcgtc agcgccccct cggggtcgaa gccggggctc atcgtctgca cgtgctggag
     5701 ggtgcggacg acgaggccgg cggcgaccag cagcacgagc gagagcgcga cctgcgcgac
     5761 gacgagcgcg ctcctgaggc gtgtgcgccg cgcgccgccc tgtgcggcgc cgtccttgag
     5821 cgcgggcacc agctccgggt tcgtcgtctg gagcgccggg acgatgccga agacgatgcc
     5881 ggtcaggacg gaggcgacga gcgagaaggc gaagacgcgc cagtcgatct ggaggtcgat
     5941 ggtgagcggg aagttgacgg gcggcttgaa ggccgtcacc acgtccacga cccacagcgc
     6001 gagcagccac ccggcggccc cgcccatgag cgccagcaac acgctctccg tcaggagttg
     6061 ccggacgagc cggaggcggc tcgccccgag cgccagacgg atggcgatct ccttgcgccg
     6121 gtcggcggcg cgcgccagca aaaggttggc gaggttggtg caggcgatga ggaggacgag
     6181 ggcgaccacg cccatcagcg cgccggtgaa gccgatgacg gggccgcgca ggatcgggtg
     6241 gatgaggccg ggcggcgtca gggtcatcgt catgccctcg ttcgtgtcgg ggtactcttc
     6301 gcccaactgc cgcgcgagga tgttcaggga ggcgacggcc tgctcgtagg tgacgccggg
     6361 cttgaggcgg ccggtggcga agaggttatg gttgtcgcgc ttctccagcc agtcgaagcc
     6421 gggctcgatc acgggctgca tcatcatcgg cgcccaaagc tccggcgtgt aggccgtctc
     6481 cgacccctgg aagccctcgg gcgcgacgcc gacgacgcta aacggatgcc cgttgagctg
     6541 cacggtcttc ccgatcaggt ttgggtcgcc gccgaagcgc gtctgccatg aggcgtggct
     6601 gaccaccacg accgggtgac gcagcggcgc cacgtcttcc tcgggcagaa aggttctccc
     6661 acgcgcggcc gagacgccga gcacgtcgaa gtagttaccc gagacgaggt agccccacac
     6721 gcgctcgctc tgcccttcgc ggctcagact catcggcgcg aagcggtgga cgaacaaacc
     6781 cgtgaagacc tcattgcggt cgcggaagtc gcggtaatcg ggataggaga aggccccgac
     6841 ctcctcgtcc ctcgccacgg ggtagaccga cgccagccgc tccggctccg ccaccggcag
     6901 cggcctcagc aacaccgtgt tgaccagcgt gaagatggcc gtgttggccc cgatgcctaa
     6961 cgccagcgac agcaccgcca cggccgtaaa ccccggactc gccttgagcc gtcgccatcc
     7021 gaaacgcacg tcctggaaga atgttcccat cagagcctcc ttcggaggta gtaagcagtg
     7081 agcagtgagc agtgagcagc aggaaatatt aaccgacgcc agagcagtcc ttctttcact
     7141 gctcactgct tactgctcac tgctcactcg tatcttagag ccaccatcgg atcgaccttc
     7201 gtcgcgcgcc gggccggcag gtagcaggcg aggagggcga cggcggcaag gagcagcgcg
     7261 acggcggaga aggtcagggg gtcggtcgcc gtcacgccga agagcaggct cgacatcaga
     7321 cgcgtcaggg cgaaggcccc gaggaggccg aggacgacgc cggcgagcgc gagcagcagc
     7381 ccgtggccga cgaccatgcc gagcacgtcg cggccttgcg cgccgagcgc catgcggatg
     7441 ccgatctcgt gcgtgcgctg cgtcaccgtg taggacatca cggcgtagat gccgacgacg
     7501 gccagcagca acgcgaccgc ggcgaacacc ccgagcaggg tggtgctgaa gcgcgcgcgc
     7561 gccaccgacg ccgagagcag ctcctccatc gtccgcacgt cggagatggg ctggtcgggg
     7621 tcaatcgact gaatctcgcg ccgcgccgcc gcggtgatgg ccgccgggtc ggccgtcgtg
     7681 cgcgcgacga tggtcatcga cgaataggcc agctccgggt gcggccagta gaccgtgggg
     7741 cgcacctcgg cggtcagatt gttgagccgc acgtcgccga cgacgccgat aatctcggtc
     7801 ggctgcggct cgttgctcat cgacacgacg agacgcttgc cgagggggtc ttcgccgggg
     7861 aagtgtttac gcaccatcgc ctcgttgacg acgacgacgc gcctgatttc ggtcgcctcc
     7921 tgctcgttga aggtgcgccc gcgggtgacg gggatgtcca tcgtgcggaa gtagttcgtg
     7981 tcggtgacgc gcacctccgt cgtcaggtct tcgcccggcg gcggcggtgg ctggcccacg
     8041 atggtgaagc gcgtcgccgc acctagccca gcgaagggca ggaagctgat ggcccccgcc
     8101 gactcgacgc ccggaacggc cccgatgcgc gccaccgcct ccttgaagaa gttgatccgc
     8161 gcttgcggct ccgcgtactt cctcggcaga ggcacctgca tcgacagcac gttcgccgcg
     8221 cggaagcccg gatcgaccga gcgcaggcgc gcgaagctcc gcaccagcag cccggccccg
     8281 acgagcatga tgagcgccag cgccgtctcg acgacgacga acgcggcgcg cagacgccgg
     8341 ctgcgcgcgc cgcccgtcga gccgcggctt ccttccttga gcgcctcgtt gttgttcaga
     8401 cgcgtggcct cgaaggcggg cgcgaggcca aagatgattc ccgtgagcac ggagacggcg
     8461 agcgtgaagc cgagcacggg caggttcagc tcgacggcgc ccagatcggt gaggtcgcgc
     8521 gggctgaggc gggccagagt ctccacgccc caccacgcca gcagcacccc gagcgccccg
     8581 ccgacgagcg cgagcagcac gctctccgtc agcagttggc gcaccactct ccagcgcccc
     8641 gctcccagcg cgcggcggat ggcgatctcc cgctggcgtg cggcggcgcg cgccagcagc
     8701 aggttcgcca cgttggcgca cgctatcagc agcaggaacg ccaccgcccc gagcaacacc
     8761 cacagcgccg tgctgatctc gcccgcgaat tgctgcctga gcggcacgac ctcgacgctc
     8821 cagcccttgt tgaaatcggc atactcccct tcgagctgtg cggcgacgga atccatctcc
     8881 gcttgcgcgc gctcgcgcgt cacgcccggc ttgagccgcg ccactgacat catgtagcgc
     8941 ccacggcgga tgcgatgttg ctcgccgagg ccgaaaggct cccacacctc cggctgcttg
     9001 cccgtgagcg accccttctg aatgagccac tggaagccgg ccggcatcac cccgacgacg
     9061 gtcgccgcct cgccgccgag cgtcatggtc ttgccgacca ccgaggggtc gccgccgaag
     9121 cgcgactgcc agaacgggta gctgaggacg acgacgggcg gcgcgttctg cgcggcgtct
     9181 tcggccgtga agccgcggcc gaggatgggt tcgacgccga ggagcgagaa catgttcggc
     9241 gtcgcgccct gcacgggcac ctccacgggg tcgcccatgc ccgtcaggtt cgagcggtaa
     9301 tcgacgaagg cggccatgtc ctcgaacacc gtgttgcgct cgccccagtc gatgaagttg
     9361 gcgggcgaga tgacgttctg gtgacggtcg cgcggccggt tggcctccca caccatcacc
     9421 agccgttcgg aatcgcggta gggcagctcg cgcaacatca gcgcgtgcac caccgagaag
     9481 atggccgtgt tcgcccccac gcccagcgcc agcgccagca ccgccacggc cgtgaacccc
     9541 ggcgacttcc acatcatccg cgcgccgtag cgcaggtctt gcatgaatgt tgtcatcacg
     9601 tcacctcttt cagagagtga tgagtgataa gtgatgagtg ataagaaaga gaaaggcggt
     9661 tttactctta tcacttatca ctcatcactt atcacttatc acttatcact tatcactcgt
     9721 atctcagtgc caccatcgga tcgaccttcg tcgcgcgccg cgcggggatg tagcaggcga
     9781 ggagggcgac taaggcgagg gcgagggaga tggcggcgaa gacgtaggcg tccgtcgggc
     9841 tgacgccgaa gagcaggctg gcgatgacgc gggccgagcc ggcggccgcg ccgaggccga
     9901 ggacgaggcc gacgagcgcg agcgccatgc cctgccgcgt aatcagccgg aggatgtcgc
     9961 cgcgctgtgc gccgagcgcc atgcggatgc cgatctcgtg cgtgcgctgc gagacggcgt
    10021 aggccagcac gctgtagagg ccggtggcgg cgagcagcaa ggcgagtatg ccgaaggcgg
    10081 cggcgagcga ggcggccatg ttcggcgccc acaaggccca cgtcaggtgc tcgcgcatcg
    10141 tcttcacgtt gtagagcggg acgcgcgcgt caaccgattt ggtcgcctcc cgcagcgcct
    10201 ccacgtatcg ggcggggtcg ccctgcgcgc gcacgaccag tgtcatcccc gactggtagg
    10261 tctggtcgag cgacacgaac atgaaggggc gcggcggctc gccgagcgtg cggtacttgc
    10321 cgtccttgac cacgccgacg acctccaacg tctcggggtc gtcgcgcccg accttcacgc
    10381 gcttgcccag agggtcttcg cccggccagt agcgcgcggc gaaagtctcg ttgatgacca
    10441 cggcctcggg ctttgcgccc ggcaggtcgt gcgcgttgaa gtcgcgcccg cgcaggagcg
    10501 gaatctgtag cgtgtcgaag tagccggggc tgacgatgtt gatctgcacg ccccggccct
    10561 cgccgggcgg tggcacaggc tgctcggccg cgtagaccgg cccccacgag gtcgagctgt
    10621 cgcccagcgg gagcaggtag gccgcgcccg cgccttcgac gccgggcaac gcttcgacgc
    10681 gctcgatgag ctggcggtaa aactccctgc cctgctccct cgtgtagccg aacagaccgg
    10741 ggttgagcga ggcagtcatc acgccctcgg acgtgaaacc gatgtcaacg gtcttggcgt
    10801 tgttgaagct cttgacgaag aggccggcgc agacaagcac gacgagcgag agcgccattt
    10861 gggagacgac gagcaggttc cgcatcgaga agcggcgacc gcgcccgcgc gcctgcggcg
    10921 tctcgccctt gagcaccggg atgaggttgg gccgggaggc ttgcagcgcg ggcgcgagtc
    10981 cgaaggcgag gcccgtgaga agtgagacgg cgagcgtgaa gatgagggcg cgcgcgtcgg
    11041 gcgcgacgct gaaggacatg gcgtagggga gcgcggggaa gaagcccgtc atcatgtccg
    11101 tgagccagaa ggagagcagc aagcccgtga gtccgccggc cgcggcgagc agcaggcttt
    11161 cggtcagcag ttggcggacg atgcgcgcgc gccccgcccc gagcgcgagc cggacggcga
    11221 tctcgcgccg ccgcgacacc gagcgcgcga gcaaaaggtt ggcgacgttg gcgcacgcaa
    11281 tcaacagcac cagcccgacg atggcgaggg ccagcccgcc gcccatccgt gccacgccgg
    11341 ccatgtcctg aaagcgcgag tcctgctccg tcaccacgtt gacgcgcgcg cccttcgtcc
    11401 gctcgttcgg gtacgcctcg ttaagtcggc gcgcaatcgt cgaaagctcg gcggccgcct
    11461 gctcgcgcgt ggcgcccggc ttgaggcggc ccatcagcga gagccagtgg ctgtcgcggt
    11521 cgttcagcca gctctggccg cccatcacct gctcctgcat cccgagcggc acgaagaagt
    11581 ccatccgcag cgcccacttc gcccccgcga agtcgtcggg cgcgacgccg acgacggtga
    11641 acggccgccc gttgacgctg actgtgcggc cgactatccc ggcgtccgcc gcgaagcggt
    11701 tgcgccacag gtgtgggccg agcactatga cggggtgcgt gccgggcgtg cgctcctcct
    11761 cgggcaggaa gccgcggccc attcgcatcc tcacgccgag cacgtcgaag aggtcggcgc
    11821 tgacgagctg cccccacacc acttcgctct gcgtctcgtc gccgacggcc gcccgcacca
    11881 tctggtggcc gacgatgccg tcaagcgttg tgctctgctc ccggtagtcc agatagtcgg
    11941 ggtaggagac gtcatcatag gcgctcccat ccgtggcctc gaagagcgcg acgaggcgtt
    12001 cggcgtcgct cacgcccgcg agcggccgca gcatgaaggc gctcacgccg ctgaagatgg
    12061 ccgtgttgac accgatgccc agtcccagcg tcagcaccgc cacggccgta aaccccggac
    12121 tcttccgcag cacgcgcaat ccgtagcgca ggtcttgcag gaatgttctc atcacaagcc
    12181 tcagaggtta gaggtcagag gtcagaggtc agtaaaaaca ggttgctttt cactaacctc
    12241 tagcctctga cctctgacct ctcattcgta tctcaaagcc accatcggat cgaccttcgt
    12301 cgcgcgccgc gccgggacgt agcaggcgag gagggcgacg agggtgagga cgagggagac
    12361 ggcggcgaag gtcagggggt cgttggcggt gacgccgaag aggaggctct gcatggcgcg
    12421 tgtggcggcg agggccgcgg cgaggcccag gcccacgccg atgagcgcga gcaccatgcc
    12481 ctgtcgcagc acgagccgca gcacgtcgcc gcgttgcgcg ccgagcgcca tgcggatgcc
    12541 gatctcgtgc gtgcgttgcg agacgacgta ggccatcacg ccgtagaggc cgagcgccgt
    12601 cagaagcaag gcgagtgcgg cgaagcccgt catcagcgtc atcacgaatc tgggcgaggc
    12661 gacgttcgtc gtcagcagcg tctccatcgt cctgaccttc cacaccggct gatccttgtc
    12721 tatcgaccac acggccgcgc gcacgtcatc ggccagactc atcggctcga ctttcgtgcg
    12781 cgccacgagc gaagcgaaga tgaacggggc ctgcgcgtag gcgtagtaaa tctgcggctg
    12841 ctgcacctcg ttgagttgga actgcttggc gtcgccgacg acgccgacga ccacgccgga
    12901 catcttgtcg gcgggcacct cgatgcgttt gccgatgggg tcttcgccgg gccagtgttg
    12961 tttggcgaac atctggttga cgatgaagac ggtgggcgag tcggccttgt cgcgctcgtc
    13021 gaaggtgcgc cccgtcgaga gtggcaggcg catggtctcg aagtagccgg gggcggccgt
    13081 gttggtgagc gcgcgcggct cgccgcccgg cgcgggcgcg gggcggtcgg gcaaaacgaa
    13141 cgtggccgag gagccgttgc cgctaaacgg tatcgcgcga atcacggagg ccgcttccac
    13201 acccggcacc tcgcgcacgc gctcgaccat tcgccggtgg aagtcccact gttgagccag
    13261 ctccggatac ttgttgcgcg gcagcctgta ttccaacgtc agaagtttct cgggcgcgaa
    13321 gccggggctg gtgttgagca ggcggtagaa gctgttgacg agcaggcccg cgccgactaa
    13381 gagcacgagc gacagcgcca cttcggagac gatgaaaatg ctgcggagtc gtccgcgcgc
    13441 gccgcccgtg cccgtcgtgc ggccttcctt gagcgactcg ttcaagtcgg gcttcgacag
    13501 gtgcagcgcc ggcatcaacc cgaacaccac gccggtgagc agggagacgc cgagcgtgaa
    13561 gagcaggacg cgcgcgtcga ggcggggcga ctgtccgccg ggaagctcgc cggggtttaa
    13621 ggacatcagc aggtcaacgc cccacagcgc gaggagcgcg ccgagcacgc cgcccgccag
    13681 actgatgagc accgactcgg tgatgaactg tctcatcaga cgccaccggc tcgcccccag
    13741 cgccgcgcgc agggccacct ccttgacgcg cgatgcgccg cgcgccaaaa gcaggttggc
    13801 gatgttggcg cagccgatga gcaggatgaa gccgacggcc gcgagcaata tcaacagcgc
    13861 cggtcgcacg tcctcgacca gcatctcctg caagccgacg aggcgagcga ccctcccgcg
    13921 gttctccgcg gggtaagccg cctccaaacg gttcgagatg gcggccac
//
LOCUS       DASVES010000279         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_620913, whole genome shotgun sequence.
ACCESSION   DASVES010000279 DASVES010000000
VERSION     DASVES010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620913"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VF240_11215"
     CDS             complement(<1..1108)
                     /locus_tag="VF240_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7175819.1"
                     /translation="MTRLSAVRLAALVRARAVSPVEVVEAHLRRVEEVNPRLNAVVTL
                     SPDAVARARALEAALARGEDAGPLAGVPVTVKDTIETVDLRTTFGSRVFVEHVPKRDA
                     PAVARLRAAGAIILGKTNVSELALDYTAENTVFGRTNNPFDDERTPGGSSGGCAAALS
                     ACLTAGSLGSDLVGSLRIPAHFCGIAALRPTAGRVPGAGHLPPLSGPFALGASLGPLA
                     RRVEDLALLFGVLTGEDLARQSEDTGDALLKDVAALLRGRRVAWYADDGATPVIPEIA
                     RAVEAAARALEDAGMSAVERRPPHVERATELWLGLFSYPTQEFLRRTYAGREEDAGSV
                     ARLLLERAERASAPTANAYFELWDERDRLRGELLE"
     gene            complement(1117..1680)
                     /locus_tag="VF240_11220"
     CDS             complement(1117..1680)
                     /locus_tag="VF240_11220"
                     /inference="COORDINATES: protein motif:HMM:NF005994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEX7175820.1"
                     /translation="MKRVLVIGSGGSGKSTLARLLGELLDVEVVHLDVEHWRPGWTEP
                     SKEDWRRKVEELLRGDEWIIDGNYSGTLETRLAACDTVVFLDLPRALCVWRALKRVVT
                     YRKGRRPDMAEGCDEKFDLKFLKWIWDYPKRTRPKVLTLLEENARARSVYRLRSRAEV
                     EGFLRGAKESGGVPREAEETGKFGGGR"
     gene            complement(1727..2632)
                     /locus_tag="VF240_11225"
     CDS             complement(1727..2632)
                     /locus_tag="VF240_11225"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7175821.1"
                     /translation="MQIETLKIFCDLVETQSFSQAAERNFVTQSAVSQQVRGLEEKFQ
                     RRLLERVRGRRELSMTEAGRTFYEASRMVLRAYESLEEGMRSLTGTVSGTLRVATVYS
                     VGLHELPPAVREFMGLYPQAKIDLEYSRTTRIVRDVLSGAVELGVVAYPEKRRGLQVV
                     PLKGDRLVLICPPAHPLAKRKRARATDLQDQDFVLFERDIPTRRATDKILRAHGVTVR
                     RAAEFDNIETIKRAVEVGLGCAIVPRPSVAGVQESGRLAVVPLVEPEWARSVGVIYRS
                     DRTLGTAALKFIELLQRHDRAKEAA"
     gene            3012..>3328
                     /locus_tag="VF240_11230"
     CDS             3012..>3328
                     /locus_tag="VF240_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX7175822.1"
                     /translation="MAVRLSRNFVLRKLHQLSGIVPLGAFLLEHLYTNSKALHPKLGP
                     EHFNSAVSDLQQIPYILFVEVTFIFIPLLYHALYGLFITIEWRPNNLAYPYGRNWFYT
                     IQRV"
BASE COUNT          572 a         1212 c          976 g          568 t
ORIGIN      
        1 attcgagcag ttcgccgcgc agacggtcgc gctcatccca caactcgaag tatgcgttcg
       61 ccgtcggcgc actcgcccgc tctgcacgtt ccaacaacag acgcgcgacg gagccggcat
      121 cttcttcgcg ccccgcataa gtccggcgca gaaactcctg cgtgggatac gaaaaaagcc
      181 cgagccacaa ttcggttgcg cgctcgacgt gcggaggtcg gcgttccacc gcagacattc
      241 ccgcgtcctc aagcgcacgc gccgccgctt cgaccgcacg cgcaatctcg gggatgacgg
      301 gcgtcgcgcc gtcgtccgca taccacgcca cgcgccggcc acgcaaaagc gccgccacgt
      361 ctttcaatag agcgtcccct gtgtcttcgc tctggcgcgc caagtcttcc cccgtcagca
      421 cgccgaacaa cagtgcgagg tcttcaactc gacgtgcgag agggccgagg ctcgcgccga
      481 gggcgaacgg gccggatagc ggcgggagat gccccgcgcc ggggacgcgc ccggccgtgg
      541 gccgaagcgc cgcgatgccg cagaagtgtg ccgggatgcg gagcgacccc acgaggtcgc
      601 tcccgagact ccccgccgtg agacacgcgc tgagcgccgc cgcgcagccg ccactcgaac
      661 cgcccggcgt gcgctcgtcg tcgaacgggt tgttcgtccg tccgaagacc gtgttctcgg
      721 ccgtgtagtc gagcgcaagc tccgacacgt tcgtcttccc cagaatgatc gccccggccg
      781 cgcgcagacg tgcgaccgcc ggcgcgtcac gcttcgggac gtgctccacg aagacgcgcg
      841 agccgaaggt cgttcgcaaa tccacagtct ctatcgtgtc cttgacggtg acgggcacgc
      901 cggcgagcgg ccccgcgtct tcgccacgcg cgagcgccgc ctccaacgct cgggcgcgcg
      961 cgacggcgtc cggcgacagc gtgacgaccg cgttcagacg cggattcacc tcctccacgc
     1021 gccgcagatg tgcctccacc acctcgacgg gcgaaaccgc gcgcgcgcgc acgagcgcgg
     1081 cgagcctcac ggcggaaagt ctcgtcaact cctccgtcag cggccacctc caaacttgcc
     1141 agtctcttcc gcttcgcgcg gaactccgcc ggattctttc gcgccgcgca gaaacccctc
     1201 gacctccgcg cgtgaacgca gacggtagac acttctggct cgcgcgttct cttccagcag
     1261 cgtcaacacc ttcggccgcg tcctttttgg gtagtcccaa atccacttga gaaacttcag
     1321 atcaaatttc tcatcgcaac cctcggccat gtccggccgc cggcctttcc ggtaagtcac
     1381 gacacgcttc aaggcacgcc agacgcagag cgcgcgcggt aggtcgagga agaccacagt
     1441 gtcacaggcc gcgaggcgcg tctccaacgt gcccgagtag ttcccgtcga tgatccactc
     1501 atcgccgcgc aagagttctt caaccttccg ccgccagtcc tccttcgacg gctccgtcca
     1561 acccggccgc cagtgctcca cgtccagatg gacgacctca acgtccaaca actcaccgag
     1621 cagccgcgcc aaagttgact tgcccgaccc accggaaccg atgaccagca ctctcttcat
     1681 cggagcacgc gcatccacgc ccgggacgac acccaccggc actttcttac gcggcctcct
     1741 tcgcacggtc gtgtcgctgt aacagctcga tgaacttgag cgcggccgtg cccagcgtcc
     1801 ggtcgctgcg gtagatgaca ccgaccgagc gcgcccactc cggctcgacc aaagggacga
     1861 cggcgaggcg tccgctctcc tgcacgccgg ccacactcgg acgcgggacg atggcgcacc
     1921 cgaggccgac ctcgacggcg cgcttgatcg tctcgatgtt gtcgaactcg gccgcgcggc
     1981 gcaccgtgac gccgtgcgcg cgcagaatct tatccgtcgc ccgccgcgtc gggatgtcgc
     2041 gctcgaagag gacgaaatcc tgatcctgca agtccgtggc gcgcgcgcgc ttgcgtttgg
     2101 cgagcgggtg cgcgggcggg cagatgagga cgagccggtc gcccttgagc ggcaccacct
     2161 gaaggccgcg gcgcttctcc gggtaggcga cgacacccag ctcgacggcc ccgctcagaa
     2221 cgtcgcgcac gatgcgggtc gtgcggctgt attcgaggtc gatcttggcc tgcgggtaga
     2281 ggcccatgaa ctcgcgcacg gcgggcggca gctcgtggag gccgacgctg tagacggtgg
     2341 ccacacgcag cgtgccgctg acggtgcccg tcagagagcg cattccctct tcgagcgatt
     2401 cgtaggcgcg cagtaccatg cggctcgcct cgtagaaggt gcggcccgcc tccgtcatgc
     2461 tcagctcgcg ccgccccctc acgcgctcca gcagacgccg ctggaacttc tcctccagcc
     2521 ccctcacctg ctggctgacg gccgactgcg tgacgaagtt gcgctccgcc gcctgcgaga
     2581 agctttgcgt ctccacgagg tcgcagaata ttttcagggt ttcgatctgc acgccttcga
     2641 gtataagtat ctctcacaac ctgccgcaag ttttttcatt tcaccttata ttctcgacgc
     2701 gctaagataa actcgcttga atatgttctc tccgcgcccc ctccgcgctc tctgcgcctc
     2761 tgcggtgagt gagcgccgca gaagattcac cgcagaggcg cagagggcgc agagttcacg
     2821 cagagagttg agcttattgt cgccaccgcg tcgcaatgag tgattgcaat tccggggagt
     2881 gtcgatataa tcatggcgtt tcacttccac ccgaagaaag ttttcgagcg cggccccgag
     2941 tcacgccagc cgttttgcgg gccgcagtaa cggggggtct ccgcccccgg ccgaggagga
     3001 cacacccggg catggccgtt cgtctgagcc gaaacttcgt cctgcgcaag cttcaccagc
     3061 tctcgggcat cgtcccgctg ggcgcgttcc tgctggagca cctctacacc aactcgaagg
     3121 cgctccaccc gaagctcggc cccgagcact tcaactcggc cgtgagcgac ctccagcaga
     3181 ttccctacat ccttttcgtc gaagtcacat tcatcttcat ccccctgctc taccacgcgc
     3241 tctacggtct gttcatcact atcgagtggc ggccgaacaa cctcgcctac ccttatggcc
     3301 gcaactggtt ctacaccatc cagcgagt
//
LOCUS       DASVES010000280         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1422552, whole genome shotgun sequence.
ACCESSION   DASVES010000280 DASVES010000000
VERSION     DASVES010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422552"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VF240_11235"
     CDS             complement(<1..1283)
                     /locus_tag="VF240_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX7175823.1"
                     /translation="MSLALLALGFMLLLVAAYRLYGGWVARQFRLDDARPTPAHEVND
                     GVDFVPTRPFYLFGQHFSAIAAAGPIAGPILACQAFGWLPCLLWIGLGVVLIGAVHDF
                     AALAASVRHGATSIAEITREQLGGKAGRAMMAFIWIALIYVTVAFTDITAGTFVAGSE
                     ELRGAAVAFNPGGAVAAASILYLALALVLGLVQKYLKPPLWLVTVIFVPAAFAVSWAG
                     TLMSNVFALDHKTWGVAILLYCCAASVAPVWALLQPRGYLGGFILYAALALGVFGIFF
                     GGYDIQQPAFKGWSAPGMTGTLFPFLFVTIACGACSGFHGLVCSGTTSKQVDKESHVR
                     AVGYGGMLAEGFVALIALVTIMIAAPELVRGKAPGTIYGEGIGRFLTLIIGEGQRNFA
                     VTFGAMAFSTFVFDTLDVSTRLGRYIMQELTGWRGR"
     gene            complement(1392..>2112)
                     /locus_tag="VF240_11240"
     CDS             complement(1392..>2112)
                     /locus_tag="VF240_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7175824.1"
                     /translation="RTFLSLIDPTELDPDFLAKVRGYRSVGTAAKINLALDALPTFTA
                     LKGSGADGAADAASSLAGRIHIGPEIDYLERAYDAAKYGDYSPEPYMDITIPTLTDPT
                     LAPDGKHVMSVYVQFAPYKLKEGDWETRGEKFGETVVKTLAAYAPNLPELVIDGQLIT
                     PADLEREYGLTGGHPLHGEMSLDQFFTFRPVIGWAQYRTPLRGLYLCGAGTHPGGGVT
                     GAPGANAGREILKDIGKTSKQ"
BASE COUNT          378 a          657 c          773 g          304 t
ORIGIN      
        1 cggccgcgcc agcccgtaag ctcctgcatg atgtagcggc cgaggcgcgt gctcacgtcg
       61 agcgtgtcga agacgaaagt ggagaaggcc atcgcgccga aggtgacggc gaagttgcgt
      121 tgaccctcgc cgatgatgag cgtgaggaag cgtccgatgc cttcgccgta gatcgtgccc
      181 ggcgctttgc cgcggacgag ttccggcgcg gcgatcatga tggtgacgag cgcgatgagc
      241 gcgacaaagc cctcggccag catcccgccg tagcccacgg cgcggacgtg cgactcctta
      301 tcgacctgct tcgaggtcgt tcccgagcag acgaggccgt ggaagccgga gcacgcgccg
      361 caggcgatgg tcacgaagag gaaggggaag agcgtgcccg tcatgccggg cgcgctccag
      421 cccttgaagg ccggctgctg gatgtcgtag ccgccgaaga agatgccgaa cacgccgagc
      481 gcgagcgccg cgtagaggat gaagccgccg agatagccgc gcggctgcaa gagcgcccac
      541 accggcgcca ccgacgccgc gcagcagtaa agcagaatag cgacgcccca cgtcttgtgg
      601 tcgagcgcga agacgtttga catgagcgtc ccggcccacg agacggcgaa ggccgcgggg
      661 acgaagatga cggtgacgag ccacagcggc ggcttcagat atttctgcac cagcccgagc
      721 acgagcgcga gcgcgagata aaggatgctc gccgccgcca ccgcgccgcc ggggttgaag
      781 gcgacggccg ccccgcgcag ctcttccgag ccggcgacga acgtgcccgc cgtgatgtcc
      841 gtgaaagcga cggtgacata gatgagcgcg atccagataa acgccatcat cgcgcgtccg
      901 gccttgcccc caagttgctc gcgcgtgatc tcggcaatcg aggtcgcgcc gtggcgcacg
      961 ctcgcggcga gggcggcgaa gtcgtgtacg gcgccgatga gcacgacgcc gaggccgatc
     1021 cacagcaggc acggaagcca accgaacgcc tgacaggcga gtatcggccc ggcgatgggg
     1081 ccggcggcgg cgatggccga gaagtgctgg ccgaaaaggt agaaggggcg cgtggggacg
     1141 aagtccacgc cgtcgttgac ctcgtgcgcg ggggtggggc gcgcgtcgtc gaggcgaaac
     1201 tgccgcgcca cccacccgcc gtagaggcgg taggccgcga ccagcaacag catgaatccg
     1261 agcgcgagca gggccaggct caagggcacc tcctaagcag gtaagcagtg agcggtgagc
     1321 agtgagcagt aaaagccagg acggcggcgc gcgtcgagca gctctgcttt aaactgctca
     1381 ctgctcaccg ctcactgctt acttgtcttc ccgatgtctt tcagaatctc ccggcccgca
     1441 tttgcgcccg gcgcgccggt gacgccgccg ccggggtgcg tgccggcgcc gcagaggtag
     1501 agtcctcgga gtggcgtgcg gtactgcgcc cagccgatga cggggcggaa ggtgaagaac
     1561 tggtcgagcg acatctcgcc gtgcaggggg tggccgcccg tgaggccgta ctcgcgctca
     1621 aggtcggccg gcgtgatgag ctgcccgtcg atgaccaact ccggcaggtt cggcgcgtag
     1681 gccgcgaggg ttttgacgac cgtctcgccg aacttttcgc cgcgcgtctc ccaatcgccc
     1741 tccttcaatt tgtaaggcgc aaactggacg tagaccgaca tgacgtgctt gccatcgggc
     1801 gcgagcgtgg ggtcggtgag cgtcgggatg gtgatgtcca tgtacggctc gggcgagtag
     1861 tcgccgtact tggccgcgtc gtaggcgcgc tccaggtagt cgatctcggg gccgatgtgg
     1921 atgcgccccg cgagcgagct tgcagcgtcg gcggcgccgt ccgcgccgga gcctttgagg
     1981 gccgtgaacg tggggagcgc gtcgagcgcg aggttgatct tcgcggccgt gcccacggag
     2041 cggtagccgc gcactttggc gagaaagtcg gggtcgagtt cggtcgggtc gatgagcgag
     2101 agaaaggtgc gg
//
LOCUS       DASVES010000281        11504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_9804, whole genome shotgun sequence.
ACCESSION   DASVES010000281 DASVES010000000
VERSION     DASVES010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9804"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF240_11245"
     CDS             <1..240
                     /locus_tag="VF240_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7175825.1"
                     /translation="LAGMLTTANAGDRYLREIADMYTELAREHNVTLIPFFLEGVAGV
                     DALNLEDRVHPNAEGTKIVAETVYRTLKPMLDKQK"
     gene            complement(643..1491)
                     /locus_tag="VF240_11250"
                     /pseudo
     CDS             complement(643..1491)
                     /locus_tag="VF240_11250"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
     gene            complement(1656..2861)
                     /locus_tag="VF240_11255"
     CDS             complement(1656..2861)
                     /locus_tag="VF240_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7175826.1"
                     /translation="MAENRPLSVLCIATFEKGQEFMRECKRQGCRVLLLTMDKLREAN
                     WPRESIDETFYLPENLPTDDLVSSVSFIARHTEIDRIVALDDFDVETGAALREHLRIP
                     GMGDSTARHFRDKLAMRVKARDNGHLVPEFVHALNRERLREFTERVASPWVLKPRSEA
                     SAVGIKKLESADELWEALEELGDRQSFYVLEQFIPGDIFHVDSIIAEREVVFTTVSKY
                     GRPPMDVAHHGGIFMTRTLPPDSDEARALDDHNRRLLRTLGLVRGVTHTEFIKGREDG
                     RLYFLETAARVGGANIAEVVEAATGVNLWAEWAKIEVRGEAGGYEPPPHRAGYAGIVI
                     SLARQEWPDTSPYTDAEIVWRLNKRHHAGLIVASPDPQRVEELLEQYVRRFCEDFFAY
                     APPPERPTS"
     gene            3347..4237
                     /locus_tag="VF240_11260"
     CDS             3347..4237
                     /locus_tag="VF240_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175827.1"
                     /translation="MEIDIPTPPGFDYKRTVMSHGWSSLPPFELDEKSLTLVRVLDPA
                     DGSPLTATIDGGGRSLKVRVPGRMGKRAQARLVRDVRHIFRLDDDLGEFYARVADDSD
                     FAWVARDGAGRLLRSPTVYEDLVKSICTTNCTWSLTEKMVSNLVGALGRPTADGRRTF
                     PTPAVMAAQPLEFYSDVMRAGYRASYFKELADRVAAGELDVESWLDSELPTPELKREM
                     KRVKGVGDYAAENLLKLVGRYDVLALDSWVRAKFTRTRGRGRACDDKKIARFYSRFDE
                     WRGLALWCDVTRDWFEESEQ"
     gene            complement(4326..4979)
                     /gene="nth"
                     /locus_tag="VF240_11265"
     CDS             complement(4326..4979)
                     /gene="nth"
                     /locus_tag="VF240_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7175828.1"
                     /translation="MNPGADRQRTARIIRKLKKSYPDARCSLNHTSPLELLVATILSA
                     QCTDERVNLVTANLFRKYRRASDYIAVPQTELEADIRSTGFFRNKAKAIQGACRLLDE
                     RHGGEVPADMEALLELPGVARKTANVVLGNAYGEASGVVVDTHVTRVSSRLGLSAQNT
                     AEKIEQDLIELVPRSDWIIFPHLLIAHGRKVCKARTPLCAECVLSPDCPSSTVSSEQ"
     gene            complement(5093..6241)
                     /locus_tag="VF240_11270"
     CDS             complement(5093..6241)
                     /locus_tag="VF240_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175829.1"
                     /translation="MRLLIAAAVFACASASVSAQGARDLLTNFPDSQFVMYVNSQRIF
                     NEALPRVVPQAKLDKMFADALREAKFDMRSIHFVAVGARFKEPMSLKTPPEFIFMLKG
                     DFNADGLLSMARLALNESGTSENYKGRTLTIFKMKKVLPPPPPAGGTGSGTGSGAGSG
                     AGSSTAPQASDLPFSELAAVSFDPNTLVVGIPAYVRAAVDAAGGDQQGRIRADLLDLV
                     TRNPDNLLSFAGDIPSSLSDMMKSVGAPQNEEINRVVASLRQVQMAVQMTAEDFGVQS
                     IVKTDTAENANGLNGLISMGLSFARMGIEGQLQKVPASKPQEREAMQAALDAITSVRN
                     AATDNEVQVNISVPQAKIATFVEREMARRKAAPAKSPVKRRVRRGRRR"
     gene            complement(6453..7577)
                     /locus_tag="VF240_11275"
     CDS             complement(6453..7577)
                     /locus_tag="VF240_11275"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX7175830.1"
                     /translation="MDNLTHSFVGLAAAKAGLERVSPYATVVCVLAANVPDADVVTLA
                     GGSLTYLKHHRGITHSIVGTLVLGLALPLLFVAAERLVARARGVEPRSRLRGLVLVSL
                     LVTASHPLLDWTNSYGLRPFLPWSGRWYYGDLVFVIDPWIWLALGAACFLLTATTVWR
                     VALWTLLALAVAAAVLLLTRDGGLEIPLAARVLWLGGLAAVVYAYRARVGERRGAAVA
                     RAALAAVVVYWGALAGLHSRASVQAETFARELAATGGETVQEVAAMPAPMNPLAWACV
                     AETERATYRYEVRLAGGGARLLSRFEKPRGADALAVERAAREPRAVVLLDFARFPVAQ
                     VRPGPAGLSLVRITDVRFGEPAEGDGGGGNFSVEIPVSAR"
     gene            complement(7696..8538)
                     /locus_tag="VF240_11280"
     CDS             complement(7696..8538)
                     /locus_tag="VF240_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175831.1"
                     /translation="MARTSKTSKKRAQQLKHDKFRDATLRGFDRLGNRMEGRGRTILY
                     ALAGLVALAVLFGLYRTWSGRAADRAGLALGNAIKIASAPVTTGTPAPGQTGPTFPSE
                     RERAQKAVEEFRKVAAEHGDPHRELARYFAAANLLMVERDKGVAELEGLTRSGNDEVA
                     ARARFALAQAREANKQYDEALALYNELLKDKNPVVPADTINFRIAAVHEQQGRKDEAA
                     EILFRMVEQARKAKDKDDKPVPLSTTAREASTKLQTLSPERYAQLPPEPTTGSGNSIQ
                     NMPF"
     gene            8906..9565
                     /locus_tag="VF240_11285"
     CDS             8906..9565
                     /locus_tag="VF240_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7175832.1"
                     /translation="MSFGEPIALRAEAEMEAAASAVSADVRAASAEAQFIERLKAGDA
                     WAFDRLVEERTGDIYALLFRLTEDREEARDLTQETFLQAFRHLSGFRGDSDLRTWLYR
                     IAVNQARNRWRWWRRRRRDTTLSLDDAGDTGQTLGARIADPDASDPEQNTLARERERT
                     LLTALGTLSRAYREVVLLRDIEGLSYEEVAATLDISVGTVKSRLSRGRQELRRRLESS
                     L"
     gene            9993..10796
                     /locus_tag="VF240_11290"
     CDS             9993..10796
                     /locus_tag="VF240_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7175833.1"
                     /translation="MNCVETQRAFSPYLDGDLTREECAALDSHLGVCVVCRIQMEQTR
                     EIVRALALVERPAPPPRLPAAISHALAAERAERAARPAPTVSGRLAHWLQPHLMPYAV
                     GAFYSLLLFVAVIGALKHQLTTLRDLAVAQRNESPERVTWLGGRGAGYDVTQPLSPSL
                     YAAARSPYTSESPSLNPRGALARMTWTPPPAGRPDDDDMIVVADVYGNGRASLAAIVE
                     PPRNPRALDDLQDALRKNPAFVPASIDGRPQSLRVVFVLQKMNVDEFTF"
     gene            complement(10929..>11504)
                     /locus_tag="VF240_11295"
     CDS             complement(10929..>11504)
                     /locus_tag="VF240_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7175834.1"
                     /translation="PDDAEVQHFLGYSLGRDARLNEAVAPLKEALRLKPDYAEARVNL
                     GSVLANTGRYQEAIDTLRPAVAARPTDPVAHFNLGIALSRHGAGRPTEIKKFYTEAHQ
                     EFTQALRLRSGWAEAHNNLGLVTGVLGDWKAAVPHHLEAARLKPDYHGAYFNLAFAYR
                     KTGDKKRAMETYQKLAAMNPAMAVKLLPLIK"
BASE COUNT         1918 a         3691 c         3845 g         2050 t
ORIGIN      
        1 ctcgccggga tgctcacgac ggccaacgcg ggcgaccgat acctgcgcga gatcgccgac
       61 atgtacactg aactcgcgcg cgagcacaat gtcactttaa tcccgttctt cctcgaaggc
      121 gtcgcgggcg ttgacgcgct caacctcgaa gaccgcgtcc acccgaacgc cgagggcacg
      181 aagatcgtcg ccgagacggt ttaccgaacg ctgaagccga tgctggataa acagaagtag
      241 cgacgagatg tagaataaag tttcggcggc agcatcaaag accgcgcctt tccttcaaga
      301 cgcggcacag gcgaaagcga gccgcagagc ggcggcagaa tgtagcccac ggcgtgagcc
      361 gtgggaacag cggctaaaat aaatgaagcc ccggaggggc gaaagaattc caggcttggg
      421 cgacacgccg gccgcgattc tttcgcccct ccggggcttt cgatttttat cgaccactgt
      481 tccacgggct ggcgcccgtg gctacattct gccgccgctc cgcggctcgt tggcccctgc
      541 gttttaactt tcgtcttctt cgcgtcggcc gcgcgcgccc gcctcttcgc ttccgtcttc
      601 gcctttagag tcgttctggt agccggcccg cgcgcgcggg tggcagcgtt cgtaggtgga
      661 gcgcaggcgc tcgccggtga cgtgcgtgta gatttgcgtg gtggcgaggt cgctgtggcc
      721 gagcatggcc tggacggagc gcgtgtcggc gccgtgctcc agcaggtgcg tggcaaaact
      781 gtggcgcagg acgtgcggcg agacgccgtg gatgccggcc cgcgcggcgt gggccttcaa
      841 catcgcccac acgttctgcc gcgtgatgat ccgcccgccc gcgcccacga agagaaactt
      901 cgactcgacg ccgccgagca ggagccgacg cgcgccctcg tgtttctgga gccacgagat
      961 cacgctgcgg cccagcggca ccttgcgctg cttgctcccc ttccccgaac agctcaccac
     1021 accctgctcg acatcgacgc cgcccaccac gagcgtcgcc agctccgaga cgcgcaggcc
     1081 cgtggcgtag agcagttcga tcatcgtccg gtcgcgcacg ccttcggggg tcgaagtgtc
     1141 cacggcttcg agcaggcgct ccatttcctc ctgcgtgaga aagcgcggga gcttctgcga
     1201 ggcgatggga gcgacgaggt cggccgtcgg gtcttgcgtg aggtgtccgt cgagcaggag
     1261 gtagcggtag aagccgcgca cggcgctgat cgtgcgcgcg atggagcgcg ggccgaggcc
     1321 gccgcgcgca aacgacatga tcatctgcga gaggtcttcc ttgccgaggt ctttcgcctc
     1381 gcgtccgacg cccgcggccc actgcgtgag tttttcgagg tcgcgccggt aggcggccag
     1441 cgagttggcc gcgagtccgc gttctacttg aacgtaggag agatgttcgc ggatcaggtc
     1501 acgcgccata aaatcacaga gtatttaaca cggatgggca agatggatag aatataaaga
     1561 ctgattcgta attacagatt tcagatttga gatttcagat ttgagatttt aaatcttccc
     1621 atcctcccca tcctgcccat ccatgttaat tcttcttaac tcgtcggccg ctccggcggc
     1681 ggcgcgtagg cgaagaagtc ttcgcagaag cggcggacgt actgttctaa gagttcttcg
     1741 acgcgctgcg ggtcggggga cgcgacgatg aggccggcgt ggtggcgctt gttgagccgc
     1801 cagacgatct cggcgtcagt atatggggag gtgtcgggcc actcctgacg ggcgagcgag
     1861 atgacgatgc cggcgtagcc cgcgcgatgc ggcggcggct cgtagccccc ggcctcgccg
     1921 cggacttcga tcttcgccca ctcggcccag aggttgacgc ccgtcgccgc ctcgaccacc
     1981 tcggcgatgt tcgcgccgcc gacgcgcgcc gccgtctcca gaaagtagag gcggccgtct
     2041 tcgcgcccct tgatgaactc ggtgtgcgtg acgccgcgca ccagtccgag cgttcgcagg
     2101 agccggcggt tgtggtcgtc gagggcgcgc gcttcgtcag agtcgggcgg gagggtgcgc
     2161 gtcatgaaga tgccgccgtg gtgcgccacg tccatcggcg gacggccgta cttcgacacg
     2221 gtcgtgaaga cgacctcgcg ctccgcgatg atggaatcga cgtggaagat gtcgccgggg
     2281 atgaactgtt cgagcacgta gaacgactgc cggtcgccca actcttcgag cgcctcccag
     2341 agttcatcag cgctctccag ctttttgatg ccgacggccg acgcctccga gcgcggcttg
     2401 agcacccacg gcgacgccac tcgctcggtg aattcgcgca gccgctcgcg gttgagcgcg
     2461 tggacgaact cggggacgag gtggccgttg tcgcgcgcct tgacgcgcat ggccagcttg
     2521 tcgcggaagt ggcgggcggt gctgtcgccc atgccgggga tacgcaggtg ctcgcgcagg
     2581 gctgcgcccg tctccacgtc gaagtcgtcg agcgcgacga tgcggtcgat ctccgtgtgt
     2641 cgcgcgatga agctgacgct gctgacgagg tcgtcggtcg gcaagttttc cggcaggtag
     2701 aaggtctcgt caatcgactc gcggggccag ttcgcctcac gcagcttgtc catcgtcagc
     2761 agcagcacgc ggcagccctg acgcttgcac tcgcgcatga actcctgccc cttctcgaag
     2821 gtggcgatgc agaggactga aagcggtcgg ttctcggcca cggcgcgact ctccacgatt
     2881 tgaaatgtgt aatggcgttt ggtgcgtgcc ggcgagcgcg cttggaaatc aggtgacgcg
     2941 cacgcgccgg cgcgggttgc atgatagcgc aggcggcgca tgcggcgaca cctcggaaag
     3001 acgtgaatcg tcaatcgtca atcgtgaatc gaaagggctg acgctcttaa actgccgatc
     3061 tacgattcaa gtcacaagac gctcaaccac gatttacgat tgacgattta cggttctgcg
     3121 agtcgtgtcc taatatcaat tctctgcgcg gcctcggcgt cctcggcgac tcggcggtga
     3181 atactcgctt cagcacactc accgcagagc cgcggaggac gccgaggccg cgcagagaac
     3241 atccccgaat taggacacca ctgttctgtg gcgtggttcg acgcgggcgt ggaaatttgg
     3301 tagcttgtgg ccgcccacaa gagagaccgc tgacgaggac tttccgatgg agattgacat
     3361 ccccaccccg cccggcttcg actacaagcg cacggtgatg agccacggct ggagcagtct
     3421 gccgccgttc gagcttgacg agaagagtct caccctcgtc cgcgtgctcg accccgccga
     3481 cggctcgccc ctgaccgcca ccatcgacgg cggcggacgt tcccttaaag tcagagtgcc
     3541 gggccggatg ggtaaacgcg cgcaggcgcg cctcgtgcgc gacgtgcgcc acatcttccg
     3601 gctcgacgac gacctcggcg agttctacgc gcgcgtcgcc gatgattccg acttcgcgtg
     3661 ggtggcccgc gacggcgcgg gtcgcctgct gcgctcgccc accgtctacg aagacctcgt
     3721 caagtcgatc tgcacgacga actgcacttg gtcgctgacc gagaagatgg tctcgaatct
     3781 cgtaggcgcg ctgggccgcc ccacggcgga cggccgccgc accttcccca cgcccgccgt
     3841 gatggccgcg cagccgctgg aattttacag cgacgtgatg cgcgctggtt atcgcgcttc
     3901 atactttaaa gagctggccg accgcgtcgc cgcgggcgag ttggatgttg agtcttggct
     3961 cgacagtgag ctgccgacgc cggagttgaa gcgcgagatg aagcgcgtga agggcgtcgg
     4021 cgactacgcg gccgagaacc tgctgaaact cgtcggccgc tacgacgtgc tcgccctcga
     4081 cagttgggtg cgcgccaagt tcacgcgcac gcgcggccgg ggccgggcct gcgacgacaa
     4141 gaagatcgcc cgcttctatt cccgcttcga cgaatggcgc ggcctcgccc tgtggtgcga
     4201 cgtgacgcgc gattggttcg aagaaagtga gcagtgagcg gtgagcagtg agcagtaaaa
     4261 actaagaggc tcggcgcgag cgtatcgccc gagcctcttg gtttttgtct tcaactgctc
     4321 actgcttact gctcactgct tactgtcgaa gaagggcagt cggggctgag aacgcactcg
     4381 gcgcacagcg gcgtgcgcgc cttgcagacc ttgcggccgt gggcgatgag caggtgtggg
     4441 aagatgatcc agtcgcttcg ggggacgagt tcgatcaggt cttgttcgat cttctcggcg
     4501 gtgttctgcg cggaaagacc gaggcgggag gagacgcgcg tgacgtgcgt atcgacgacg
     4561 acgccggagg cttccccgta ggcgttgccg aggacgacgt tggcggtctt gcgggcgacg
     4621 cccggtagtt cgagcagcgc ctccatgtcg gcggggactt cgccgccatg ccgctcatcg
     4681 agcaggcggc aggcgccttg tatcgccttc gccttgttgc ggaagaagcc ggtggagcgg
     4741 atgtcggctt cgagttccgt ctgcgggacg gcgatgtagt cggaggcgcg gcggtatttg
     4801 cggaacagat tggcggtgac gaggttgacg cgctcgtccg tgcattgcgc ggagaggatg
     4861 gtggcgacga ggagttcgag cgggctggtg tgattgagac tgcaacgagc gtcggggtag
     4921 gactttttca gcttgcggat gatgcgcgcg gttctttgcc tgtcggcgcc ggggttcaag
     4981 cgagtagctc ctaccgttaa gattaagaag gggaaggggg aagggataaa gggtaaggga
     5041 ggcccgctgt ctaagcttca ccctttccca ttaccccttc cccttttccc tactacctgc
     5101 gccttccccg ccgcacgcgt cgcttaaccg gactcttcgc cggcgcggcc ttgcggcgcg
     5161 ccatctcacg ctccacgaac gtggcgatct tcgcctgcgg gacggagatg tttacctgca
     5221 cctcgttgtc ggtggccgcg ttcctgactg aggtgatggc gtcgagcgcc gcctgcatcg
     5281 cctcccgctc ctgcggtttg ctggcgggca ccttctgcaa ctggccctcg atgcccatcc
     5341 tcgcgaagct caggcccatc gagatgaggc cgttgaggcc gttcgcgttc tccgccgtgt
     5401 ccgtcttgac gattgactgg acgccgaagt cttccgccgt catctgcacg gccatctgca
     5461 cctgccggag cgaggcgacg acgcggttga tctcctcgtt ctgcggcgca cccaccgact
     5521 tcatcatgtc agacagagag gaggggatgt cgccggcgaa gctcaggagg ttgtcggggt
     5581 ttcgggtgac gagatcgagg aggtcggcgc ggatgcggcc ctgctggtcg cctccggcag
     5641 catcgacggc cgcgcgcacg taagccggga tgccgacgac gagcgtgttc gggtcgaagc
     5701 tgacggcagc gagttccgag aacgggaggt cggaagcttg cggtgcggtg ctgctcccgg
     5761 cgccgctccc ggcgccgctc ccggtgccgc tcccggtgcc gccggcggga ggcggcgggg
     5821 ggaggacctt cttcattttg aagatggtca gcgtgcgccc tttgtagttc tcgctcgtgc
     5881 cgctttcatt cagggcgagg cgcgccatcg agagcaggcc gtcggcgttg aagtcgccct
     5941 tcaacatgaa gataaattcc ggcggcgtct tgagcgacat cggctccttg aagcgcgcgc
     6001 cgacggcgac gaagtggatc gagcgcatgt cgaactttgc ttccctcagc gcgtcggcga
     6061 acatcttgtc gagtttcgcc tgcgggacga ccctcggcag ggcctcgttg aagatgcgct
     6121 gggagttgac gtacatcacg aactgggagt cggggaagtt cgtgaggagg tcgcgcgcgc
     6181 cctgcgccga aacacttgcg gaggcgcagg cgaagacggc cgcggcgatg aggagtctca
     6241 tggaagtcct ggcaatcatc cgaaaatcat cctttcacga cagctgacgt ttaagggccg
     6301 cgcgggcggg ggcgtgtgtc gccaaaagtt tccgcgcccg gccgttcaca cacgacgcat
     6361 aactcacgcc ggcgattgtg cccgaaagct tacgcgcccg acaaggtgaa agttgcacgc
     6421 gcctcccctt caagcggatt gcggattcgg cgtcaacgcg cgctcaccgg tatctcgacg
     6481 ctgaagttgc cgccgccccc gtccccctcg gccggctctc cgaacctcac gtccgtgatg
     6541 cggacgagcg ataggcccgc cggccccggc ctcacctgtg cgacggggaa gcgcgcgaag
     6601 tctaacagga ctacggcgcg cggttctcgc gcggcgcgct cgacggcgag ggcgtccgcg
     6661 cccctcggct tctcgaagcg tgagaggaga cgcgcgccgc cgcccgcgag tcgcacctcg
     6721 taccgatacg tcgcccgctc cgtctcggcg acgcaggccc aggcaagcgg gttcatcggc
     6781 gcgggcatgg cggccacttc ctgcacagtc tccccgccag tcgcggctaa ctcgcgcgca
     6841 aacgtctcag cttgaacgct tgcgcgcgag tgcaagccgg cgagcgcgcc ccagtagacg
     6901 acgacggccg ccagcgccgc gcgggccacc gccgcgcccc tccgctcgcc gacgcgcgca
     6961 cgatacgcgt agacgaccgc agccaacccg ccgagccaga gcacgcgcgc ggcgagcggt
     7021 atctccaacc cgccgtcgcg cgtcagcaac aggacggccg cggcgaccgc cagagcgagc
     7081 agcgtccaga gcgcgacgcg ccagacggtc gtggccgtga gcagaaagca ggccgcgccg
     7141 agcgcgagcc aaatccaggg gtcgatgacg aagacgaggt cgccatagta ccagcgcccg
     7201 ctccacggaa ggaaggggcg caggccgtag ctgttcgtcc agtcgagcag tgggtgcgag
     7261 gccgtcacca gaagtgagac gagcacgagt ccgcgcaggc gggagcgcgg ctccacgccg
     7321 cgcgcgcgcg ccacgagccg ctcggcggcg acgaagagca gcggcagcgc caagccgagt
     7381 acgagcgtcc ccacaatcga atgcgtgatg ccgcggtgat gtttgaggta ggtcaggctc
     7441 ccgcccgcca gcgtcaccac gtcggcgtcc ggcacattag ccgccagcac gcagacgacc
     7501 gtcgcgtaag gcgacacgcg ctccagaccc gccttcgcgg ctgccagccc cacgaacgag
     7561 tgcgtgagat tgtccatgga agaagctgtt agctgttagc tattagccag aggtcggggg
     7621 tgcgtcgtcg ccaacaaaca aaactttggc tcgctcactt ccggctaaaa gctaagagct
     7681 aatggctaac agcttttaga acggcatatt ctggatgctg ttgcctgagc cggtggtcgg
     7741 ctcgggcggg agttgggcgt agcgttcggg gctgagggtc tggagttttg tggaggcctc
     7801 gcgggccgtg gtcgagagcg gcacgggctt gtcgtccttg tctttggcct tgcgcgcctg
     7861 ctcgaccatg cggaagagaa tttcagcggc ctcgtccttg cggccctgtt gctcatggac
     7921 ggcggcgata cggaagttga tcgtgtccgc ggggacgacc gggttcttgt ccttcaacaa
     7981 ctcgttgtag agggcgaggg cttcgtcgta ctgcttattg gcctcgcgtg cctgcgccag
     8041 cgcgaaccgg gcgcgcgccg ccacctcgtc gttgccgctc cgggtcagcc cttcgagttc
     8101 ggccacgccc ttgtcacgct cgaccataag gaggttggcg gcggcgaagt agcgcgcgag
     8161 ttcgcggtgc gggtcgccgt gctcggcggc gaccttgcgg aactcctcga cagccttctg
     8221 cgcgcgctcg cgctcgctgg gaaaggtcgg ccccgtctgg cccggcgcgg gcgtgcccgt
     8281 ggtgacgggc gccgaggcga tcttgatggc gttgcctaag gcgagaccgg cgcggtcggc
     8341 cgctcggccg ctccacgtgc ggtagaggcc gaagagcacg gcgagggcga cgaggccggc
     8401 gagggcgtag aggatcgtgc ggccacggcc ctccatgcga ttgccgaggc ggtcgaagcc
     8461 gcgcagcgtc gcgtcgcgaa acttgtcgtg cttgagttgc tgggcgcgtt tcttagaagt
     8521 tttggaagtt cgggccactg tgttccttcg tgcaagacct cactgcgagg cgcgttcttt
     8581 tcgcgggtgg cgaagcaaag gcaccaaatg tagcgacgcg ctcgcggcgt gtcaagcaag
     8641 cgccggaaac gaagtttgac ggccccacgg acgcgtgcta cgctcccgcc ccgaaccgcg
     8701 gcgcgccccg cgtgcccctc gcccctcgcc gctttttttg aaactcgtta agcgttgata
     8761 ttttacgact tgtgcgatgg gccgcggcat ccaagcggcg gcgggaatta tttgtggaac
     8821 tgacgatgac aacgcggtgt ctaaagtgtc ggttgagggg ggttcggcgg cggcaccgga
     8881 accttccgcc cgccggagga cgcctgtgag cttcggagag ccaatcgccc tccgcgcgga
     8941 agcggagatg gaggccgccg cgtcggcggt gtccgccgat gtgcgcgccg cgtccgccga
     9001 agcgcagttc attgaaagac tcaaggccgg cgacgcgtgg gccttcgacc gcctcgttga
     9061 agagcgcacg ggcgacatct acgccctgct cttccgcctg accgaagacc gcgaggaagc
     9121 cagagacctg acgcaggaga ccttccttca ggcgttccga cacctctcgg gctttcgcgg
     9181 cgactcagac ctgcggacgt ggctctaccg catcgccgtc aatcaggcgc gtaatcgctg
     9241 gcgctggtgg cgccgacgcc gacgcgacac gacgctgtcg ctcgacgacg cgggcgacac
     9301 cggccagacg ctcggcgcga ggatcgccga cccggacgcc tccgacccgg agcagaacac
     9361 gctggcccgc gagcgcgagc gcacgctgct cacggcgctc ggcacgctct cacgcgcata
     9421 ccgcgaggtc gtcctcctgc gcgacatcga ggggctgagc tacgaagagg tcgccgccac
     9481 gctcgacatc agcgtcggca ccgtcaaatc gcgcctctcg cgcggccgac aggagctaag
     9541 gcgcaggctg gaaagttctt tgtaactgaa gctgttagct attagctgtt agctgttagc
     9601 caaacaccaa aagctaacag ctaatagcta acggcttctt cggatggctg atctaccccg
     9661 gcttcacctg aaaacccggt gccaggtggg tcaggaagtg accgcgccgc tgcggttcgg
     9721 ccgcagggtt ggacgcacaa ccggaaaagc gcgcgggcgg ctttgaaaac gtggccagca
     9781 acacccgtta tcaagtcgcc gcacgcacgc cggacggttt gtgccgaacg ccccacgccg
     9841 cacgagtgga cagtgttgct tccttttaca agggagcagc cggggttgat gagccatccg
     9901 ggcaagagag gtcagaggtc agaggttaga ggtcagtcaa agacctgctt tttaactaac
     9961 ctctgacctc tgacctctga cctctgattt ttatgaactg cgtggagaca cagagggctt
    10021 tttcgcccta cttggacggg gacttgacgc gcgaagagtg tgcggcgctc gactcccacc
    10081 tcggcgtgtg tgtcgtctgc cgcatccaga tggagcagac gcgcgagatc gtccgcgcac
    10141 tggcgctggt cgagcgcccc gccccgccgc cgcgactccc cgccgccatc tcgcacgcgc
    10201 tggccgccga gcgcgccgaa cgcgccgcgc gacccgcgcc caccgtctcg ggccgactag
    10261 cccactggct ccagccgcat ctcatgccct acgccgtcgg cgccttctac tcgctgcttc
    10321 tgttcgtcgc agtcatcggc gccctcaagc atcaactgac cacgctgcgc gacctcgccg
    10381 tggcccagcg caatgaatcg cccgagcggg tgacgtggct cggcggccgt ggcgccggct
    10441 acgacgtgac gcagccgctc tcgccctcgc tctacgcggc ggcgcgctcc ccgtacacct
    10501 ccgagtcgcc cagcctcaat ccgcgcggcg cgctggcgcg aatgacctgg acgccgccgc
    10561 ccgcgggccg tcccgacgac gacgacatga tcgtggtcgc cgatgtctac ggcaacggcc
    10621 gcgcctctct ggccgccatc gtcgagccgc cgcgcaaccc gcgcgccctc gacgacctgc
    10681 aagacgccct gcgtaagaac ccggctttcg tccccgcctc catcgacggc cgtccccagt
    10741 cgctgcgcgt cgtcttcgtc ctccagaaga tgaacgttga cgaattcacc ttctgattcg
    10801 ttcaaagcgg gaacagaaaa aggcaaaagt aaaaaggcaa aaggcaaaag tggaagaggc
    10861 aacctgcctt ctcacttttg ccttttgcca ttttactttt gccttccccc cgttcccgtc
    10921 tttctctttt acttaatcag tggcaggagc ttgacggcca tcgcggggtt catcgcggcg
    10981 agcttctggt aagtctccat ggccctcttc ttgtcgccgg tcttgcggta ggcgaaggcg
    11041 aggttgaagt aggcgccgtg gtagtcgggc ttgagacggg cggcctcgag gtgatggggc
    11101 accgccgcct tccaatcccc gagcacgcct gtaacgaggc ccagattgtt gtgcgcctcg
    11161 gcccagccgg agcgcaagcg tagggcctgc gtgaactcct ggtgcgcctc cgtgtagaac
    11221 tttttgatct ccgtggggcg gccggcgccg tggcggctga gggcgatgcc gaggttgaag
    11281 tgcgccacgg ggtcggtggg gcgcgcggcg actgccgggc gcagcgtgtc gatggcctcc
    11341 tgatagcgtc ccgtgttggc gagcaccgag cctaagttga cgcgcgcctc ggcgtagtcc
    11401 ggcttgaggc gcagggcctc cttgagcggc gcgacggctt cgttcaagcg agcgtcgcgg
    11461 ccgagcgagt agccgaggaa gtgttgcacc tcggcgtcgt cggg
//
LOCUS       DASVES010000282         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_591856, whole genome shotgun sequence.
ACCESSION   DASVES010000282 DASVES010000000
VERSION     DASVES010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591856"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..839)
                     /locus_tag="VF240_11300"
     CDS             complement(339..839)
                     /locus_tag="VF240_11300"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7175835.1"
                     /translation="MNTADIRQLYDYNTWANARVLDAVAALTPEQFTRDMASSFPSVR
                     DTLAHIMAAEHVWLARWRGESPKRLLDAAEFPTLGDLRARWAEIERELSAFVRDVTDE
                     SLERVIAYTNTRGEEWRYALGAMMQHVVTHSAYHRGQVTTMLRQLGARGVLTDYLAYH
                     DETSRK"
     gene            920..1663
                     /locus_tag="VF240_11305"
     CDS             920..1663
                     /locus_tag="VF240_11305"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX7175836.1"
                     /translation="MLDALSREKGSRMKSEKISELTTNRLSVYLRCLNLLAAAGIRTV
                     SSKTLAEQFQLNSAQIRKDLGYFGEFGVRGVGYDVSELRRHLTQILGLDRQHLVGIVG
                     AGRLGTALANYNGFTASNFRIVALFDNDPAKIGRRVGEADALVHDVQGLAGVVGAERI
                     DVCVIAVPARAAQGVLNQITSAGVKAVLNFAPVRLRARAGVKVKTVDLTIQLESLSYF
                     LARPQPAQTTSPARALPRRPAARRERQKL"
     gene            1742..2566
                     /locus_tag="VF240_11310"
     CDS             1742..2566
                     /locus_tag="VF240_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7175837.1"
                     /translation="MDEQTARREIISVGRLMYERGYVVSSDGNVSVRLDDGRIVATPT
                     QVNKGRMTEEMLAITDAEGRALSDRRASSELAMHLLIYRERADVKSVCHAHPPHGSAF
                     AVAGLAIDQPILSEVILTLGCVPLADYGTPSTDELTDAMRPLVKHHNALLMANHGAVA
                     YGADLWQAFDRLETLEHTAKIAILARALGGARNLPPDSIEKLIDIRERAGYLDERARC
                     QACGYLHETRVTCATGDRPPPPTAPRAAMNGAGKISLTREELVELLVEAARTQRLS"
     gene            2700..2984
                     /gene="eutM"
                     /locus_tag="VF240_11315"
     CDS             2700..2984
                     /gene="eutM"
                     /locus_tag="VF240_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HEX7175838.1"
                     /translation="MAQEALGMVETKGLVAMIEAADAMVKAANVTLVGYEKIGAGFVT
                     AIVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHGSVDESLPVGLNS"
     gene            3115..3387
                     /locus_tag="VF240_11320"
     CDS             3115..3387
                     /locus_tag="VF240_11320"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX7175839.1"
                     /translation="MIIARILGTVVASQKDERLKGGKLLIVKPLNLDGTDAAGYVVAI
                     DTVGAGFHERVLVVAGSSARLADGMKDKPVDAAIVGVIDTIDLMND"
     gene            3578..3868
                     /locus_tag="VF240_11325"
     CDS             3578..3868
                     /locus_tag="VF240_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX7175840.1"
                     /translation="MQLARVIGSVVASVKNDSLEGRKLLVVQSLDANLRPQGAPLVAL
                     DAIGAGASDLVFWCRGKEASFPFRREDTPTDCTIVAIVDSDAHVTRGNGGRS"
     gene            3865..4170
                     /locus_tag="VF240_11330"
     CDS             3865..4170
                     /locus_tag="VF240_11330"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX7175841.1"
                     /translation="MILARVLGNVVATQKNARYDGARVMMCQPVTPAGEETGATILAL
                     DSVDAGAGDMVLIVQEGWGASTAATGEAGAAIDSAIVGVVDHIDLLADEPIRKPLFE"
     gene            4167..4664
                     /locus_tag="VF240_11335"
     CDS             4167..4664
                     /locus_tag="VF240_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175842.1"
                     /translation="MSSSEQEQIRALAERVARRLASQAGEAGAARTGSHVADAGDLSA
                     VRDGLADIRRRLAHIESHIGHEGSPESGTRATSETSRASSAGNTTHGCGADPDAASGQ
                     TRASSLWLSGTYVPATEHPSQERFGIDEAVSEMVDFFEREKTCNLEPGDKPCDHCGMC
                     SSRGF"
BASE COUNT          854 a         1514 c         1543 g          815 t
ORIGIN      
        1 tcctccatgt cgtagaaggt ggagacgggg atggtgactt cgtagccgac cccggccacg
       61 tcgagcacga cgctcgtggc gtgcttggac agaatggttc ctgagagatg tgcgatcatt
      121 actgtggcgt cttaaaagag agttggattt cgggagcgcc tctttgttcg gcgagaattt
      181 tagtcgaacg gaggcgaaaa gcaaaaagcc gtgaatcgtc aatcgtcaat cgtcaatcgt
      241 tttcagtcgc cgccggcccg gctcgcgctc cgctgttcgg gcggttttag ctgaaagcta
      301 agagctgata gctaatagct atttattttg cgttccgttc atttacgcga agtctcgtcg
      361 tgataggcga ggtagtctgt gagcacgccg cgcgcgccga gctgacggag catcgtcgtc
      421 acctgcccgc gatgataggc ggagtgtgtg acgacgtgct gcatcatcgc tccgagcgcg
      481 tagcgccact cctcgccccg cgtgttcgtg taggcgatga cgcgctcaag cgactcgtcc
      541 gtcacgtcgc gcacgaacgc cgacaactcg cgctcgatct ccgcccaacg cgcgcgcagg
      601 tcgccgagcg ttggaaactc cgccgcgtcg agcagacgct tgggcgactc gccgcgccag
      661 cgcgccagcc agacgtgctc ggcggccatg atgtgagcca gcgtgtcgcg caccgaggga
      721 aagctgctcg ccatgtcgcg cgtgaactgc tccggggtga gcgccgccac ggcgtcgagc
      781 acgcgcgcgt tggcccacgt gttgtagtcg tagagttgcc ggatgtctgc tgtgttcatc
      841 ggtctgctcc cgccgcccgt ttgaggttcg gagccgcgtt gcgccacagg cgtctttccg
      901 cgccggcccc gcccgcgtta tactcgatgc gctctcccga gaaaagggtt caaggatgaa
      961 gtcggagaaa atctctgagc tgacgacgaa ccgcctgagc gtctacctgc gctgcctgaa
     1021 cctgctggcc gccgccggca tccgcacggt ctcctcgaag acgctggccg aacagtttca
     1081 gctcaactcg gcgcagatac gcaaagacct cggctacttc ggcgagttcg gcgtgcgcgg
     1141 cgtcggctac gacgtgtcgg agctgcgccg ccacctgacg cagattctcg gtctcgaccg
     1201 ccagcacctc gtcggcatcg tcggagccgg ccggctgggg acggcgctcg ccaactacaa
     1261 cgggtttacg gcctcgaact tccgcatcgt cgccctcttc gacaacgacc ccgcgaagat
     1321 cgggcggcgc gtgggcgagg ccgatgccct ggtgcacgac gtgcaggggc ttgcgggcgt
     1381 cgtcggcgca gagcggatcg acgtgtgcgt catcgccgtg ccggcgcgcg cggcgcaggg
     1441 cgtgctcaac cagattacgt cggccggcgt caaggcggtg ctcaatttcg cgcccgtgcg
     1501 tctgcgcgcg cgggccggcg tgaaggtcaa gacggttgac ttgacaatcc aactggagag
     1561 cctatcgtac ttcctcgcgc gcccgcagcc ggcgcagacc acaagccccg cgcgcgcact
     1621 gccccgtcgt ccggcagcgc gacgcgagag acagaagttg tgaaactttt aagtggcgcg
     1681 gggccgtgtg gcgtagttcg tcgcgcactt tcggctgatg acattcagga agtaaaagca
     1741 gatggatgag cagacggcgc gacgcgagat tatcagcgtc ggccggttga tgtacgagcg
     1801 cggttacgtc gtctcctcgg acggcaacgt cagcgtgcgc ctcgacgacg ggcgcatcgt
     1861 cgccacgccg acgcaggtca acaagggacg catgaccgag gagatgctcg ccatcaccga
     1921 cgccgagggc cgcgcactta gcgaccgccg cgcctcgtcc gagctggcca tgcacctgct
     1981 catttaccgc gagcgcgccg acgttaaatc cgtctgccac gcgcacccgc cgcatggctc
     2041 ggccttcgcc gtcgcgggcc tcgccattga ccagccgata ctctccgaag tgatcctcac
     2101 gctcggctgc gtgccgctcg cggattacgg cacgccctcg acggacgaac tcaccgacgc
     2161 catgcgcccg ctcgtcaaac atcacaacgc gctcctgatg gcgaaccacg gcgcggtggc
     2221 ctacggcgcc gacctctggc aggcgttcga ccgtctggag acccttgagc acaccgccaa
     2281 aatcgccatc ctcgcgcgcg ccctcggcgg cgcgcgcaac ctcccgcccg actccatcga
     2341 gaagcttatc gacattcgcg agcgggccgg ctacctcgac gagcgcgcgc gctgtcaggc
     2401 ttgcggctat ctccatgaga cgcgcgtcac ctgcgcgacg ggcgaccgcc cgccgccgcc
     2461 aaccgccccg cgcgcggcga tgaacggcgc tggtaaaatt tcgctgacgc gcgaagaact
     2521 cgtcgagctt ctggtcgagg cggcgcgcac gcagaggctt tcgtaaatcg tgaatcgtaa
     2581 atcgtaaatc gtgaacagat aagatatgct ggaggcgact tacggcggac atttcacagc
     2641 agggcgacag ttcctgaacg atttacgatt cacgattcac gatttacgag gagaagataa
     2701 tggctcaaga agcactggga atggttgaga cgaagggcct cgtggcgatg atcgaggcgg
     2761 ccgacgcgat ggtcaaggcc gccaacgtga cgctcgtcgg ctacgagaag atcggcgccg
     2821 gcttcgtcac cgccatcgtg cgcggcgacg tggccgccgt gaaggccgcc actgacgccg
     2881 gggcagcggc cgcgcgacgc gtcggcgaac tcgtcagcgt ccacgtcatc ccgcgcccgc
     2941 acggcagcgt tgacgagagc ctgcccgtgg gcctcaatag ttaaaagccg tgaatcgtca
     3001 atcgtcaatc gtcaatcgtt tcggttgacc gtcgcccgac ttgggactcg acggctcaag
     3061 tcgagacggt tccaaccacg atttacgatt cacgattgac gattgacgaa tcaaatgatt
     3121 atcgcgcgca tcctcggcac ggtcgtcgcg tcgcagaagg acgagcggct caagggcggg
     3181 aagctgttga tcgtcaagcc gctcaacctc gacgggacgg acgcggccgg ctacgtcgtc
     3241 gccatcgaca cggtgggggc gggctttcac gagcgcgtgc tcgtcgtcgc cggctccagc
     3301 gcccgcctcg ccgacgggat gaaggataag cccgtggacg cggccatcgt cggcgtcatc
     3361 gacacgattg acctgatgaa cgattaggaa gcgattagcg attagctgtt agctatcagc
     3421 taaagactgt cagtggtcag tcgtccgttg tcagttgtct gtgtcgggcg attcgccgct
     3481 gtcgttggga agattcaagc tgggcgacgg ggacaactga ccacggacaa agaacagctt
     3541 ttagcttaca gctaatcgct aacagctaac cgctcttatg caacttgcac gggtcattgg
     3601 cagcgtggtg gcgtcggtga agaatgattc gctggagggg cgcaagctgc tcgtcgtcca
     3661 atcgctcgac gcgaacctcc ggccgcaggg cgcgccgctg gtcgcgctcg acgccatcgg
     3721 cgcgggcgcg tccgatctcg tcttctggtg ccgcggcaag gaggcgagct tcccgttccg
     3781 gcgcgaggac acgccgactg actgcaccat cgtcgccatc gtcgattccg acgcgcacgt
     3841 gacgcgcggc aatggggggc ggagttgatt ctggcgcgtg tgctcggcaa cgtcgtggcg
     3901 acgcagaaga acgcgcgcta cgacggcgcg cgcgtgatga tgtgccagcc cgtgaccccc
     3961 gccggcgagg agacgggcgc gaccattctg gcgctcgact cggtggacgc cggcgcgggc
     4021 gacatggtgc tgatcgtgca ggaggggtgg ggcgcctcga ctgccgcgac gggcgaggcg
     4081 ggcgcggcaa tcgattccgc gattgtcggc gtcgttgatc acatcgactt gctagcggac
     4141 gagccgatac ggaaaccctt gtttgaatga gcagtagcga acaggaacag attcgcgcgt
     4201 tggccgagcg cgtcgcgcgt cgcctcgcct cgcaagctgg tgaggcgggc gcggcccgca
     4261 ccggctcaca cgtcgccgac gccggcgacc tctcggcggt gcgcgacggg cttgccgaca
     4321 tccgccgccg gctcgcgcac atcgagtcgc acatcggcca cgaggggtcg cccgaatcgg
     4381 gcacgcgagc gacgagcgag acgagccgcg caagttcggc aggaaacaca acacacggct
     4441 gcggggctga ccccgacgcc gcgagcggcc agacgcgcgc ctcctctctc tggctgagcg
     4501 gaacttacgt ccccgccacc gagcacccga gtcaggagcg tttcggcatt gatgaggccg
     4561 tctcggagat ggtcgatttc ttcgagcgcg agaagacgtg caatctcgaa cccggcgaca
     4621 agccctgcga ccactgcggc atgtgcagct cgcgcgggtt ctaaactcgg cccgcaaagt
     4681 aaaacaacaa acgaacgttt agaggcggtc tctgaccgcc gtctca
//
LOCUS       DASVES010000283         5707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_447981, whole genome shotgun sequence.
ACCESSION   DASVES010000283 DASVES010000000
VERSION     DASVES010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447981"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2503
                     /locus_tag="VF240_11340"
     CDS             <1..2503
                     /locus_tag="VF240_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX7175843.1"
                     /translation="QATPATWRLLVESGWAGSAGLKILCGGEALARSLAAELAGRGAA
                     VWNLYGPTETTIWSAVGRVEAGAGEGLVSVGGPIANTQMYVLDAELEPAPVGVVGELY
                     IGGEGLARGYLNRPELTAERFVPHPHGEEAGARLYRTGDVARYRSDGGMEVVGRVDQQ
                     VKLRGHRIELGEVESVLNGCEGVSQAVVLLREDAPGDRRLVAYLSSGAEAESITTTEL
                     REYLRERLPEYMMPSAFVMLREWPLTPNGKVDRKALPAPDRSTRPDGAEHAAPRSRAE
                     KVLADIWAEVLGMESVGINDNFFELGGDSILGIQAVARANRAGFELIPRQLFQHQTIA
                     ALASAAGPAPVLRTEQGAVTGSVPLTPVQHWFFEKDLPDPHHSNQSVLLEVRGGLNIS
                     ALEESVRLLLAHHDALRLRFARTETGWQQWHAAADEPVPVSLVVLPSATDEEYREAVS
                     AAAAEAQATLNLFHGPLMRVTAFRSERGLPDRLHVAIHQLAVDGVSWRVLFEDLQLCY
                     EELAEGRTPRLPPKTTSYQYWAQRLSDYAQTEAVRQEAAYWLWETGTLSEPLPIDFEG
                     RGESGHADSARTVSVSLGAEATAGLLYDAPRSYRVQVNDVLLTALARSFARWTANNSL
                     MVCVESHGRQDIFGDVDFTRTVGWFTALFPVRLTLPGEAHPGEQLKAIKEQLRRVPNH
                     GLGYGLLRYLCRDAQTARSVGELASPEVLFNYFGNVDQALDEAAPFTLCEDEARRERS
                     PSESRGFMFEVNTYVTDGSLVSNWTYDSNLHRRETVEALAEGFMESVRLLADYCRGAE
                     TTAGYTPSDFPKSKLNQTELDHLLSKISASRGELA"
     gene            2500..>5707
                     /locus_tag="VF240_11345"
     CDS             2500..>5707
                     /locus_tag="VF240_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX7175844.1"
                     /translation="MNSTKDSIEDIYPLSPMQQGILFHALLNVDTAVYVEQLTCAVSG
                     PLDVGAFKSAWQRLIERHSILRTAFVWEGLREPMQYVVREASFGCVEDDWRGLPAAEG
                     EERLRQYLRQDRARGMALNEAPLMRAALFRRAEQDYQFVWSYHHLLLDGWSGALLFQE
                     ALTLYKSLRRGETPRLDPVRPFGDYIEWLQRQPLSAAESFWRGALRGLSTPTPLWLDR
                     APTGGLDQASRHDRQETILPAALTRELTSLARRNQLTLNTMIQGAWALLLGRYSGQED
                     VMFGATVSGRPAALAGVETILGNFINTLPVRVQLNPDAVIIDWLKELQDAQAEARQYD
                     YVTVAQIQGWSDVPRGMPLFESVVVFENYPTNSAFGAGAWEEEAELRLSEIRSDDLIN
                     FPLALGAEPGTASELTLRLVYDARRFDDAVVGDMLGHLRTTLEVFARDPFQPLKAVTL
                     LTEVELRRLLFEWNDTRREYSPYTSVLELFDAQVGRAPDSVAVCFEGERLTYGELDAR
                     ANMLARRLRRLGVGPETLVGLHMERSLEMVVSILGVLKAGGAYLPLDPSYPHERLDRM
                     LEEARPPVVLTQDALAEQLPAASAHVLCVDSCWDSIAEEGGEPTGCTVSGGNVAYVIY
                     TSGSTGKPKGVMNTHEAILNRLLWMQDAFGLTAADSVLQKTPFGFDVSVWEFLWPLMY
                     GARLVVARPGGHQDSSYLVELIVEEKITVTHFVPSMLRIFLEEKGVEACERLRHVVCS
                     GEALPAELVSRFYERCQAQLHNLYGPTEAAIDVTSWRCERGNDAQTVPIGRPISNTQI
                     YLLDTDLRPVPVGVPGELHIGGVNLARGYLGWAELTAEKFIPDPFGPQPGARLYKTGD
                     IARFRPEGQIEYLGRTDAQVKIRGFRIELGEIETTLSRHAQVREAVVLAREDTPGNKR
                     LVAYLTLAPGAQVNITELRGFLQMSLPEYMAPAAFVFMESFPLTESGKIDRRRLPPPD
                     EDRSRLTTPLVGPRTSAEAELATMWRELLGVERVGVHDNFFELGGHSLMLTQLASRID
                     GVFQVQLPLRVLFNVPTIVEMTTAIAARQMEQADPEELT"
BASE COUNT          982 a         1720 c         1961 g         1044 t
ORIGIN      
        1 gcaggcgacg ccggcgacgt ggaggctgct cgtcgagtcg gggtgggcgg ggagcgcggg
       61 gttgaagatt ctgtgtgggg gcgaggcgtt ggcgcgctcg ctggccgctg agttggcggg
      121 gcgcggcgcg gcggtgtgga acctctacgg gccgacggag acgaccatct ggtcggcggt
      181 ggggcgggtg gaggcggggg cgggtgaggg gttggtgagc gttggagggc cgatagccaa
      241 cacgcagatg tacgtgctcg acgctgagtt ggagccggcg ccggtggggg tggtggggga
      301 gttgtacatt ggtggagagg ggctggcgcg agggtatctg aaccggccgg agctgacggc
      361 ggagaggttc gtgccacatc cgcacgggga ggaggcgggg gcgaggctgt accggacggg
      421 ggacgtggcg cgctaccgct cggacggcgg gatggaagtc gtaggacgtg tggatcagca
      481 ggtgaagctg cgcggccatc gcatcgagtt gggagaagtt gagagcgtgc tgaacgggtg
      541 cgaaggcgtc agtcaggcgg tcgtgctgct cagggaagac gcgccgggcg acaggcgtct
      601 ggtggcctac ctctcaagcg gcgcggaggc tgagtcaatc accacgactg agctgcgcga
      661 atacttacgc gagcggttgc cggaatacat gatgccttcg gccttcgtca tgttgcgcga
      721 gtggcccctg acgccgaacg gcaaagtaga ccggaaggcg ctgcccgcgc ccgacaggtc
      781 aacgcgcccg gacggcgcgg aacatgccgc gccgcgcagt cgcgcggaaa aagttttggc
      841 ggacatctgg gcggaagtgc tgggcatgga gagcgtcgga atcaacgaca actttttcga
      901 gttgggcggc gattcgatcc tcggcattca ggccgtcgct cgcgccaacc gtgcgggttt
      961 tgaactcatc ccgcgccagc tattccagca tcagacgatt gccgctctgg cgtccgctgc
     1021 cggccccgcg cccgtgttgc ggactgagca gggcgcggtc acgggaagcg tcccgctgac
     1081 gcccgtgcag cattggttct ttgaaaaaga tttgcccgac ccacaccact ccaaccagtc
     1141 ggtgctgttg gaggttcgcg ggggcttgaa catctccgcg ctcgaagagt ccgtgaggct
     1201 gctgttggcg catcacgacg cgctgcgcct gcgcttcgcg cggactgaga ctggctggca
     1261 acagtggcac gcggccgccg acgagcctgt gccggtctct ttagtggtgc tgccctccgc
     1321 gaccgatgag gaataccgcg aggccgtttc ggccgcggcc gccgaagcgc aggccacgct
     1381 gaatttgttt cacggcccgc tgatgagagt cacggccttc cgctcagaaa gagggctgcc
     1441 cgaccggctg cacgtggcca tccaccaact ggccgtggac ggtgtctcgt ggcgcgtttt
     1501 atttgaagac ctacaactct gttacgaaga actcgcggag ggacgaaccc cccggcttcc
     1561 tcctaagacc acctcttatc aatactgggc gcaacggctg tccgactacg cgcagacgga
     1621 ggccgtgcgg caggaggccg cctactggct gtgggagacg gggactttga gcgagccgct
     1681 tcccatcgac ttcgagggac ggggcgagag cggccacgcg gattcggcgc gcacggtctc
     1741 ggtgtcgctc ggggcggaag cgacggccgg attgctctac gacgcgccgc gctcctaccg
     1801 cgtgcaggtc aacgacgtgc tcctcacggc gctggcgcgg agcttcgcgc gttggactgc
     1861 caacaattct ttaatggtct gcgtggagag ccacgggcgg caggacatct tcggcgatgt
     1921 cgattttacg cgcaccgtgg gttggttcac ggccctgttc cctgtccgtt tgacgctgcc
     1981 gggcgaagcg caccccggcg aacaactcaa ggcaatcaag gaacaattgc gccgcgtccc
     2041 taatcacggc ctcggctacg gcctcctgcg ttacctttgc cgcgacgcac agaccgcgcg
     2101 cagtgtgggc gagcttgcca gtcccgaagt cttattcaac tatttcggca atgtcgatca
     2161 ggcgttggac gaggccgcac ccttcaccct ctgcgaggat gaagcgcggc gcgaacgcag
     2221 cccgagtgaa agtcgtggct ttatgtttga agttaatacc tacgtcaccg acgggagcct
     2281 cgtctcgaac tggacgtatg actcgaatct ccaccggcgc gaaaccgtcg aagcactggc
     2341 cgaggggttc atggagtccg tgcgcctgct cgccgactac tgccgcgggg ccgagacgac
     2401 ggccggctat accccttcgg actttccgaa gtcgaagctc aatcagaccg agttggatca
     2461 tctgttatcg aaaatcagtg cgtcgcgcgg tgaattggca tgaactccac gaaggattca
     2521 atcgaagata tttaccccct ctcgccgatg cagcagggga tactcttcca cgccctgttg
     2581 aacgtagaca ctgcggtcta cgtcgagcaa ttgacctgcg cggtcagcgg gccgctcgac
     2641 gtgggcgcct tcaagtcggc ctggcagcgt ctgatcgaac gccactctat actgcgcacg
     2701 gcttttgtct gggagggttt gagggaaccc atgcagtacg tcgtgcgtga ggcgagtttc
     2761 ggatgcgtgg aggacgactg gcggggtctg ccggcggccg aaggagaaga acgccttcgt
     2821 caatatctgc gacaagaccg tgcgcggggc atggccctca acgaagcccc gctcatgcgt
     2881 gccgccctct tccgccgcgc ggagcaggac tatcagttcg tctggagtta ccaccatttg
     2941 ctcctcgacg ggtggtcggg ggcgctgctg tttcaggaag cgctcacgct ctacaaatcc
     3001 ttgcggcgcg gcgagacccc gcgcctcgac ccggtccggc ccttcggcga ttacatcgag
     3061 tggttgcaga ggcagccact ttcggccgcc gagagcttct ggcgaggggc gctgcggggg
     3121 ctttcaacgc cgacgccgct ctggctcgac cgcgcgccga ctggcggcct cgatcaggcg
     3181 agccgccacg atagacagga gacgatactc cccgccgcgc tgacacggga gttaacctcg
     3241 ctggcccggc ggaatcagtt gaccctcaac accatgattc agggggcatg ggcgctgctc
     3301 ctcggtcgtt atagcgggca ggaggatgtc atgttcggag ccacggtgtc gggtcgcccg
     3361 gccgcgctcg cgggcgtcga aaccatcctc ggtaatttca tcaacaccct gcccgtgcgc
     3421 gtgcagctca accccgacgc ggtgattatc gattggctga aggagttgca ggacgcgcag
     3481 gccgaggcgc ggcagtacga ttacgtcacc gtcgcacaga ttcaggggtg gagcgacgtg
     3541 ccgaggggca tgccgttgtt cgagagcgtc gtcgtctttg agaattatcc gaccaactcc
     3601 gctttcggcg ccggagcttg ggaagaggag gctgaactca ggctgagcga aatccgctcg
     3661 gacgatttaa tcaacttccc gctggcgctg ggcgcggagc cggggacggc atcggagttg
     3721 acgctcaggc tggtctacga cgcgcggcgc tttgacgacg cggtcgtcgg ggacatgctc
     3781 gggcacctgc ggacgacgct ggaggttttc gcccgcgatc ccttccagcc cctgaaggcg
     3841 gtgacgttgt taacggaggt cgaacttcgc cggctcctct ttgagtggaa cgacacgcgg
     3901 cgcgagtatt cgccctacac gtccgtcctt gagttgttcg atgcacaggt ggggcgagcg
     3961 cccgacagcg ttgcggtctg cttcgaggga gagcgcctga cctacggtga gctggacgcg
     4021 cgggcgaaca tgctcgcacg tcgtctgcgg cggctggggg tggggccgga gacgctggtt
     4081 ggccttcaca tggagcgctc ccttgagatg gtcgtgtcga ttctcggcgt gctcaaagcc
     4141 ggcggggctt acctgccact cgatccctcg tatccgcacg agcgccttga caggatgttg
     4201 gaggaggcgc gtccgccggt cgtgctgact caagatgcgt tggcggagca actccccgcc
     4261 gccagcgcgc acgtcctgtg cgttgactcg tgctgggatt cgattgccga ggagggcggc
     4321 gagccaacgg gctgcacggt gtcgggcggc aacgtggcat acgtgatcta cacgtccggc
     4381 tcgactggca aacccaaagg tgtgatgaac acccacgagg ccattttaaa tcgactgctt
     4441 tggatgcagg acgccttcgg tctgacggcg gcggacagtg tcttacagaa aacgcccttc
     4501 ggcttcgacg tgtcggtgtg ggagtttctt tggccgctga tgtacggagc gcgcctcgtc
     4561 gttgcgcggc ccggcggcca tcaggacagt tcatatctcg tcgagttgat agttgaagag
     4621 aagatcacgg tcacgcattt cgttccctcg atgctgagga tttttctgga agagaaaggg
     4681 gtcgaagcgt gcgagcggct gcgccacgtc gtgtgtagcg gagaggcgtt gcccgccgaa
     4741 cttgtgagtc gattttacga acgctgccag gcccaacttc ataacctgta cggcccgacg
     4801 gaggcggcca tcgacgtgac gagctggcgg tgcgaacgcg ggaacgatgc tcagacggtt
     4861 cccatcggac ggcccatctc gaacacacaa atctacctgc tcgatacgga cctgcgcccc
     4921 gtgcccgtcg gcgtgcccgg cgagttacac atcggcggcg tcaatctggc gcgaggctat
     4981 ctgggctggg cggagttgac ggccgagaaa ttcatccccg acccgttcgg cccgcagccc
     5041 ggcgcgcggc tctacaagac cggagacatc gcccggttcc gccccgaagg ccagatcgaa
     5101 tacctcgggc gcaccgacgc gcaggtgaag attcgcggct tccgcatcga gttgggggag
     5161 attgaaacaa ccttgtcccg gcacgcgcag gtgcgcgagg ctgtcgtcct cgcgcgtgag
     5221 gacacgcccg gcaacaagag gctggttgct tacctgaccc tcgcccccgg cgcgcaggtc
     5281 aacatcaccg agctgcgcgg cttcctgcaa atgtcgctgc ccgagtacat ggcgccggcg
     5341 gccttcgtat tcatggagtc tttcccgctg acggagagcg ggaagatcga ccgacgccgg
     5401 ttgcctccgc ccgacgagga caggtcgcgt ctgacgacgc ctctggtcgg gccgcggaca
     5461 tcagcggaag cggaactcgc gacgatgtgg cgcgaactcc tcggcgtcga gcgcgtcggc
     5521 gttcacgaca acttcttcga gttgggcggg cactcgctga tgctcacgca actcgcctcg
     5581 cgtattgacg gcgtctttca ggtgcagctt cctctgcgcg tgctcttcaa cgttccgacc
     5641 atcgtcgaga tgacgacggc catcgcggcg cgacagatgg agcaggccga cccggaggaa
     5701 ctcaccc
//
LOCUS       DASVES010000284        18257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_632105, whole genome shotgun sequence.
ACCESSION   DASVES010000284 DASVES010000000
VERSION     DASVES010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18257
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632105"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1024
                     /locus_tag="VF240_11350"
     CDS             155..1024
                     /locus_tag="VF240_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7175845.1"
                     /translation="MQFGDYRVEMVPDAEFRLDGGAMFGVVPRSLWSRYCPPDEENRV
                     RLSANCLYVEAAGERILVETGMGDKWSARQAEMYGVERARPLGESLRALTGCAPETIT
                     IVVNTHLHFDHAGGNTTLGDDGRVVPTFPNARYFVSAAEHEHAEHPHERDRASYLPEN
                     WRPLAESGQLELKPDAYEVVPGLSVETVPGHSRTMQCVRLERAGRTLFCFADLVPTRA
                     HVPFAWVMGYDLYPVETLEAKKRLLPQAARENWLCLFYHDPEAPLCRVVEEDGRLRAE
                     GFVEGQDGFIPGE"
     gene            1044..1919
                     /locus_tag="VF240_11355"
     CDS             1044..1919
                     /locus_tag="VF240_11355"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX7175846.1"
                     /translation="MTTAKIGIIGGSGLYQMEELKDVEELKVDTPFGPPSDAFITGTL
                     EGVRVAFLPRHGRGHKLLPTELPFRANIYAMKLLGVERILSASAVGSLQEQYAPLDMV
                     IPAQFFDRTRARAHESTFFGEGIVAHITFAHPVCDELGGVVEESCRAAGVNVHRGGTY
                     LCMEGPAFSTKAESNVYRGWGMDVIGMTNLQEAKLAREAEICYSTLALVTDYDCWHED
                     HDAVSVEAVVEYLNKNVRNAQNVMREAVRRLSGQTRSCACGSALKNAIFTAPDLWPEA
                     TTKKLEAIIKKYQRK"
     gene            2057..2566
                     /gene="bamD"
                     /locus_tag="VF240_11360"
     CDS             2057..2566
                     /gene="bamD"
                     /locus_tag="VF240_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7175847.1"
                     /translation="MKKFFLTCCGLALLVFSAGGARAQGGGATKGPDPTVIADAELEK
                     EALHNLEVAKHYFKMKKAYVAAATRAEEIVAGYPGFTRTDEALYIAGMSNVYLAEGKG
                     KQKSTAPADKLRDEARAYLSRIVEEFPESDFRGRAEEELRKLGGAKAGGAAARDDSRK
                     PQLKTASQP"
     gene            2623..3564
                     /locus_tag="VF240_11365"
     CDS             2623..3564
                     /locus_tag="VF240_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7175848.1"
                     /translation="MSLLVVGSVAFDAVETPFGKRERMLGGSATHFSLAASFFADVRV
                     VGVVGGDFGAEEMAVFERQHINTDDIERVPDGQTFFWRGRYDFDLNTAHTLDTQLNVF
                     ADFKPKLSDASKQARLVFLGNIQPDLQSEVRAQVAGAQLVALDTMNYWINTVPEQVKR
                     SIAGVHVVIINDAEARQLAGEPNLIRAAREILSWGPRALVVKRGEFGAAMFTPETYFA
                     IPAYPLESVFDPTGAGDTFAGGFMGYLASHDEIDEETMRRAMTFGSVMASFNVEEFGT
                     DRVSRLTQDEINRRFREFKRMTHFEEIPFERAVRATK"
     gene            3701..4090
                     /locus_tag="VF240_11370"
     CDS             3701..4090
                     /locus_tag="VF240_11370"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX7175849.1"
                     /translation="MFCPVCESEYEAGISVCPDDNTELVERLTPENTLRDHSEARFVP
                     LHNIGSPAEAEMIVDILTQNGIRAVLQSGGMDAFSPLLSTTAPGALVLVDERDLDRAQ
                     EIYVSFFGQDTTPLTGATAEEETTSDE"
     gene            4205..4597
                     /gene="cutA"
                     /locus_tag="VF240_11375"
     CDS             4205..4597
                     /gene="cutA"
                     /locus_tag="VF240_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX7175850.1"
                     /translation="MDETDSGAIMVVMTAGSREEASRLAEMLVGSHLAACVQILPEIE
                     SVYRWEGKVHREPEVLLFAKTTRACFAELEREVRALHTYDTPEIIALPVAIGSAPYLE
                     WLNDVVMTPYGARAAERHAESQGEATDQ"
     gene            complement(4591..5343)
                     /locus_tag="VF240_11380"
     CDS             complement(4591..5343)
                     /locus_tag="VF240_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7175851.1"
                     /translation="MAHTKAKHKTEAAGRAVTQDEQLLESPRADEFKRSDTWRVFRIM
                     GEFVEGFDELATVTRGVSLFGSARTPADSPMYEAARETAALLAREGFAVITGGGPGIM
                     EAANRGALDAGGLSIGCNIELPFEQRPNPYQTRSITFDYFFVRKTMFIKYSTAFIIFP
                     GGFGTLDELFEALTLIQTRKIRNFPVVLYGSQYWELLLRWTKEVLLAEGMISETDLNL
                     LHLTDSPEEAVRIVVESQNSLRQLDDASAYDY"
     gene            complement(5431..6192)
                     /locus_tag="VF240_11385"
     CDS             complement(5431..6192)
                     /locus_tag="VF240_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX7175852.1"
                     /translation="MLLENKFGIVFGVANKRSIAWATAQACTEAGARLAFNYQSERLK
                     ENVESLTAGTDAPLIQCDVTKPEEVEQAFSVVRERFGRLDFLVHSLAFAPREALEGEY
                     VETTHDAFLMALDISAYSLVTLARAAAPLMTEGGSIISMTYYGAEKVVPHYNVMGVAK
                     AALEASTRYLASDLGPRNIRVNAVSAGPINTLAARGVGNLSTMLKHHAEHSPLKRNVE
                     AREVGNAALFLLSPLSSGITGEVLHVDCGYNIMAF"
     gene            complement(6214..6612)
                     /locus_tag="VF240_11390"
     CDS             complement(6214..6612)
                     /locus_tag="VF240_11390"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX7175853.1"
                     /translation="MKRVVLGLLPALCLLFVAAPAQKKRQAAKDPCPGEYTQYEMNVC
                     AHKEFQAADAALNRIYNQFSSKLDEGRRAKLKEAELAWIKYRDGNCEFVSSEYEGGSM
                     RPMIHSFCLADVTRARTAELKAQLKELERE"
     gene            complement(6643..6816)
                     /locus_tag="VF240_11395"
     CDS             complement(6643..6816)
                     /locus_tag="VF240_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175854.1"
                     /translation="MVTITFPDRETEKRALAFLIGRFSGHVLNSGEHLIPAAALEALA
                     DQHIPFMVKGKTT"
     gene            complement(6846..7829)
                     /locus_tag="VF240_11400"
     CDS             complement(6846..7829)
                     /locus_tag="VF240_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7175855.1"
                     /translation="MSDIQPVNEQNNDTIRCRYCGQLNQTRDNSNAQARCGRCRLTLS
                     GEPHKKFAELDKHEYIHPADSRALAALKTIPGIDSALKKLLEVTGESAIRIIFTASAV
                     KVTPKQCPDLYAKLQIACSTLGVDMPELFVQQNPVVNAFTGGVKRPVIVLHSALIERL
                     NDEETLAVLAHEVGHIHAEHVLYLTAAHLLELLANAAISTTPLAGIIAQLISGTMRAA
                     ILAWARRAELSCDRAALLVVQDPDVIGRAMMKLCGGTFASRVDYDQFLEQARDFQKNY
                     DQKALDRFWADIINAGLTHPFPVWRVSEILKWVESGEYQRVMSGQPASAAA"
     gene            complement(7985..9670)
                     /gene="purH"
                     /locus_tag="VF240_11405"
     CDS             complement(7985..9670)
                     /gene="purH"
                     /locus_tag="VF240_11405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX7175856.1"
                     /translation="MTEPNTVGLKPIRRALLSVSDKAGLIDFARALRGHGVEILSTGG
                     TARALREAGVEVRDVSDLTGFPEMLDGRVKTLHPRVHGGLLGVRANPEHAASMREHGI
                     EPIDMVVVSLYPFEQTVEREGVTLAEAVEQIDIGGPAMIRSAAKNYGDVAVVSSPYSY
                     EKIVDELKAQRGSLSLLTRARLARQAFMLTAEYDRAIFSFLASTLPGLAKNRMQTGDL
                     ISEDVQCMSEDFTVTDYDKLQAVELRVEAQGSFPKNITLLCEKKTDLRYGENPHQKAA
                     LFVTSESGGVSAAEVLGGKEMSYNNYVDADAAWQLVCDFEETACAIIKHTNPAGVGTA
                     ATTEAAYRLALATDPVSAFGGVVAFNRTLDETAARALTEIFTEVVVAPDYEDGALEVL
                     KSKKNLRVLRAGPAEPPEGLDFKRITGGILIQTRDAHRLAPDQLKVVTRRAPTEEETR
                     ALLFAWTVCKHTKSNAIVYAREGQTVGVGAGQMSRVDSARLGAMRAQLTLAGSVVASD
                     AFFPFRDGLDEAARHGVTAVIQPGGSVRDDEVIAAADEHGLAMVFTGLRHFRH"
     gene            complement(9894..10961)
                     /locus_tag="VF240_11410"
     CDS             complement(9894..10961)
                     /locus_tag="VF240_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7175857.1"
                     /translation="MASNGAANPQIADSPRSARLLFVTATLFAALVSAHLHARAQTDA
                     APPDETIRVNTDLVSVPVIITDRRARRVAGLTAADFEIIDEGSGVEISYFAVGAERVA
                     LVFLLDASGSTRDIIAGQREAALALFSRFGKRSRVSVIHFQERPALSLPFTADLEAAR
                     AAFRFVALPDRRTAIFDAALAAVRVFDTPGADPAERRIVILISDGLDTASRVRPSAVI
                     DEARARGVAFYVIHTPLFWIQGGRLGPRPTAKGFRDLALKTGGQLFVVGDAETSSGAQ
                     TPHDFWPVFRAIEDDLRAQYVLGYYAGEAQRTGLPRRVEVRLKTRDAGKLRVRALRDT
                     YVLERRPEGGGAPPPSATPEP"
     gene            complement(10975..11109)
                     /locus_tag="VF240_11415"
     CDS             complement(10975..11109)
                     /locus_tag="VF240_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175858.1"
                     /translation="MPHTRWISPRASHTERTKVVSLVKIIVITIAVILSILFFGRFLF
                     "
     gene            11342..11719
                     /locus_tag="VF240_11420"
     CDS             11342..11719
                     /locus_tag="VF240_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175859.1"
                     /translation="MRGRGLRGNFGRWSPAASVLLLVLFAHAFLVNATHCHRFARAVA
                     PGAEQEIGVSGREDLGRQSEAGGDAQCVLCRLQRNFVTDFHRVSTPVGPTRQECQPYR
                     LPPALSSNARAFSVPQGRAPPLA"
     gene            11971..14163
                     /locus_tag="VF240_11425"
     CDS             11971..14163
                     /locus_tag="VF240_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7175860.1"
                     /translation="MAAVVLLFAPARALAQMGGSLYSLPQTNGAIKGTVTLAEGGTAL
                     HNAIVTVVQLRRSVQTDENGAYEFTPVPPGAYTVLAHMEGFPDATETVTVAAGAPATL
                     DIKLSLSGLREEITVTATGTEQSTFESLQSVTTLDTIRLTEESHPSIGEVLDKEPGVA
                     KRSFGPGSSRPVIRGFDGDRVLITQDGASTGSLASQSADHAEPIDVLSLERLEVVKGP
                     ATLLYGSSAVGGVVNAVTGHDYAHDGWRGYLTGVGGTTNNQGGASGGLEYGTGKWMLW
                     GNGSAQRTGDYNTPLGRVEQSFSRSGSGTLGVGRYEEKNFLNFTYGYDRRRYGIPFAS
                     FFETGGEETGSDVSIKTKRQDFKFNGGFRDLDTPVSGFRMTVDYSDYGHRELEGEEVN
                     TQFSNKLFMYRGVFDQRKRGSLSGSFGFSGFHRDYDTVGAEALAPPTTQNNFAVFGLE
                     VIDFERVKFQFGGRVEHNSYNPDGLLARSYTGFSGAAGVRVQLWTNGAFVANYTHSYR
                     APALEELYNNGPHVGNLTFEIGNPLLTRESNDGVDFSLRHESKRVRGAANFYYYNIKD
                     FVFLAPTGAIEDNLIEARYGQEDSRFMGTELNLELAPRENLWLTFGFDAVDAQLKASD
                     TPLPRIPPMRARFGIDWRYKGLSLRPEAVFVKDQDQLFPTETRTPGYALFDVVASYTL
                     ARPHYAQIFAVNAFNLGDRLYRNHLSFIKDLAPEIGRGVRFTYTLRYF"
     gene            14355..15827
                     /locus_tag="VF240_11430"
     CDS             14355..15827
                     /locus_tag="VF240_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175861.1"
                     /translation="MPSKFGKLYLIPLLLILGITSSHAQTLDIKLSVATLNPPRLRVE
                     GARTNGARTWSFLNVYGGALGLGERIENLTLADAEGKEVAVRKRAPGEFEAVREATRF
                     SYEIRLNPPANASDAAHVSWLTDERGLLMLADVLPGPLRDARVRLALPPGWSVSTTEV
                     VNSEGTYEIADATLSVFLLGRDLRSNPSRRGEMEFASVITGEWAFGDDVAASSATDIL
                     KEYEELMGGVPLRRARIFVLPFPLATPAQSWSAETRGGTVVILSGRLPSKTFALSRLD
                     GILAHELLHLWVPNGLSLDGEYDWFYEGFTLYESMRHGLRRNQLTFQDYLNAIGRAYD
                     GYKAARVADKISLVEASRRRWTLAPSFIYDKGMLVAFLYDLTLTRRSGGKNSLDDVYR
                     TLFRRHGLKTAREDGSRAVLNALGGVGGMQDFARRYVEGQADIDLAAELAPFGLRLEA
                     TGTRTRVVVSDSLDKSQRDLLKKFGYNEKSSPSPRRPRDN"
     gene            15952..17067
                     /locus_tag="VF240_11435"
     CDS             15952..17067
                     /locus_tag="VF240_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175862.1"
                     /translation="MLKQQCLLLLLAVLSFTSGCGALAGKEETGVVIARRAQVRSSMA
                     VVAADLKEVVRGDVLEILDSITAENGERWLHVRTRDAESIEGWVEARNIIPENMIERS
                     RQLAKEDQGVPPQASGQLRANTNIRFTPDRGGNDNILMKLESGARFYIIGWKRVAKPK
                     TSETSESDDAPKSGVAQGRSARRGREDAEPKETEDPDELWYKVRLPATLSPAPAGWVL
                     GKQVELTVPSDIIFYRTGREFVAWQRLDGEGEDASATTHVDKDAAKETRPGSWVILEK
                     SSTRDKTETDAPEFDRIFVLGYDKDRQEHYTAYRSPDLDGLLPLRVEGQGDNKIFTVK
                     IRDNGQTRDVRFKAYIDSRGLLKVESLDELPKIQKGR"
     gene            17601..>18257
                     /locus_tag="VF240_11440"
     rRNA            17601..>18257
                     /locus_tag="VF240_11440"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3489 a         5526 c         5917 g         3325 t
ORIGIN      
        1 ggactagttt ctttccaaga acagcggctt ctttattacg ggatggcgga ttcacgtatt
       61 ggtctggcta ttacaaaagc aactccactg gagagcagag cctaacatat tatcttgtct
      121 gtttattctg actcctgact ccccggttca cattgtgcaa ttcggcgatt accgtgtaga
      181 gatggtgccg gacgcggagt ttcgtctcga cggcggggcg atgttcggcg tcgtgccgcg
      241 ctctctgtgg tcgcgctact gcccgcccga tgaggagaat cgcgttcgac tgagcgcgaa
      301 ctgcctctac gtcgaggccg cgggcgagcg catcctcgtc gagaccggga tgggcgacaa
      361 gtggtctgct cgacaggcgg agatgtacgg cgtcgaacgc gcgcgcccgc tcggcgagtc
      421 gctgcgcgcg ctcacgggtt gcgcgcccga gacgattaca atcgtcgtca acacgcacct
      481 gcacttcgac cacgcgggcg ggaacacgac gctcggcgac gacggccgcg tcgtcccgac
      541 tttccccaac gcgcgctact tcgtctccgc ggccgagcac gagcacgccg agcacccgca
      601 cgagcgcgac cgagcgagct acctgccgga gaactggcgg ccgctcgcgg agtccggcca
      661 actggagctg aaaccggacg catacgaggt cgtgccgggg ctgtcggtcg agacggtgcc
      721 gggacattcg cgcacgatgc agtgcgtgcg tcttgagcgt gcggggcgga cgctcttctg
      781 cttcgccgac ctcgtcccga cgcgcgcaca cgtccccttc gcgtgggtca tgggctacga
      841 cctctacccg gtcgaaacgc ttgaggcgaa gaaacgtctg ctgccgcagg ccgcgcgcga
      901 aaactggctg tgcctgttct atcacgaccc cgaagcgccg ctctgccgcg tcgtcgaaga
      961 agacggacgg ctgcgcgctg aagggttcgt ggaagggcag gacggattca ttcccggaga
     1021 gtgaggcgaa gaagcgaaac gacatgacga ctgcgaagat cggaatcatc ggcgggagcg
     1081 gcctttacca gatggaggag ctaaaggacg tggaggagtt gaaggtcgat acgcccttcg
     1141 gcccgccgtc cgacgccttc atcacgggca cgctcgaagg cgtccgcgtg gccttcctgc
     1201 cgcgtcacgg gcgcgggcac aaactgctgc cgacggagtt gcccttccgc gctaacatct
     1261 acgccatgaa gctgctcggc gtcgagcgca tcctttcggc ctcggccgtc ggctccttgc
     1321 aggagcagta cgcgccgctc gacatggtca tccccgcgca gttcttcgac cgcacgcgcg
     1381 cacgcgccca cgagtcaacc ttcttcggcg aaggcatcgt cgcccacatc accttcgcgc
     1441 acccggtgtg cgacgaactc ggcggcgtgg tcgaggagtc gtgccgcgcc gccggggtca
     1501 atgttcatcg cggcggcacc tacctgtgca tggaggggcc ggcgttctcg accaaagccg
     1561 agtcgaacgt ctaccgcggt tggggcatgg acgtgatcgg gatgacgaac ttgcaggagg
     1621 cgaagctcgc ccgcgaggcc gaaatctgct actcgacgct cgcgctcgtc accgactacg
     1681 actgctggca cgaagaccac gacgccgtct cggtcgaagc ggtcgtcgag tatctcaaca
     1741 agaatgttcg caacgcgcag aacgtgatgc gcgaagccgt ccgccgcctc agcggccaga
     1801 cgcgttcctg cgcctgcggc tccgccctca aaaacgccat cttcaccgcc cccgaccttt
     1861 ggcccgaggc gacaacgaaa aagctcgaag cgatcatcaa aaagtaccag aggaagtgat
     1921 aagtgataag tgataagtga taagaagcag ttagctctta gcgattagct aacagccaac
     1981 agctaaaggc taacagcttc ttttctttct tatcactcat cactcatcac ttatcaccat
     2041 tgaaagaggt gtcgagatga agaagttttt tctgacgtgc tgtggattgg cgctgttggt
     2101 gttcagcgcg ggcggcgcga gggcgcaggg cggaggggcg acgaaggggc cggacccgac
     2161 cgtcatcgcc gacgcggagc ttgagaagga agccctgcac aatcttgagg tcgccaagca
     2221 ctacttcaag atgaagaagg catacgtcgc cgccgccacc cgcgccgagg agatcgtcgc
     2281 cggctaccct ggtttcacgc gcactgacga agccctctac atcgccggga tgagcaacgt
     2341 ctatctcgcc gagggcaagg gcaagcagaa gtccacggcg ccggccgaca agctgcgcga
     2401 cgaggctcgc gcttatctgt cgcgcatcgt cgaggagttc cccgaaagcg acttccgcgg
     2461 gcgcgcggag gaggagttgc gcaagctcgg cggcgcgaag gccggcggtg ctgcggcccg
     2521 cgacgacagc cgcaagccac agcttaagac ggcctcgcaa ccgtaagatc gaatccttag
     2581 ttcttcgctc tcaaaactta ttcgcaggag aatcagatta aagtgtcatt gctggttgta
     2641 ggttcggtgg cgttcgacgc ggtcgagacg cccttcggta agcgtgagag gatgctcggc
     2701 ggctcggcca cgcacttctc tctggcggcg agtttctttg cggacgtgcg cgtggtcggt
     2761 gtcgtcggcg gcgacttcgg cgcggaagag atggccgtct tcgagcgcca acacatcaac
     2821 acggacgaca tcgagcgcgt ccccgacggc cagaccttct tctggcgcgg ccgttacgac
     2881 ttcgacctca acaccgcgca cacgctcgac acgcagttga acgtcttcgc cgacttcaag
     2941 ccgaagctct cggacgcgtc gaagcaggcg cgcctcgtct tcctcggcaa catccagccc
     3001 gaccttcagt cggaagtgcg cgcgcaggtg gcgggcgcac aactggtcgc gctcgacacg
     3061 atgaactact ggatcaacac cgtccccgag caggtcaaac gctccatcgc cggcgtccac
     3121 gtcgtgatta tcaacgacgc cgaggcgcgt cagctcgcgg gcgagccgaa cctcattcgc
     3181 gcggcgcgcg agattctgtc gtgggggccg cgggcgctcg tcgtcaagcg cggcgagttc
     3241 ggggcggcga tgttcacacc cgagacctac ttcgccatcc cggcctatcc gctggagtcg
     3301 gtcttcgacc cgacgggcgc cggcgacacc ttcgcgggcg gcttcatggg ctatctcgcc
     3361 agccacgacg agatcgacga ggagacaatg cgccgcgcca tgaccttcgg ctccgtgatg
     3421 gcctccttca acgtcgagga gttcggcacc gaccgcgttt cccgactcac gcaggacgag
     3481 atcaaccgcc gcttccgaga gttcaagcgc atgacccact tcgaggagat tccgttcgag
     3541 cgcgcagtgc gagcgacgaa ataaggcaaa agtaaaaagg caaaaggcaa aagtgaagag
     3601 gccacgcttc ctcacttttg ccttttgcct ttttactttt gccttttgag ctataatgcc
     3661 ggccgtgtaa taaaaacgtc ggagccgaga ggggttttta atgttctgtc ctgtctgcga
     3721 gtcggagtac gaggccggga tcagcgtgtg tcccgacgac aacacggagc tggtcgagcg
     3781 gctgacgccg gagaacacgc tgcgcgacca cagcgaggcg cgcttcgtgc cgctgcacaa
     3841 catcgggtcg ccggccgagg ccgagatgat cgttgacatc ctgacgcaga acggcatccg
     3901 cgcggttctc cagtcgggcg ggatggacgc cttctcgccc ctgctttcga ccaccgcccc
     3961 cggcgcgctc gtgctcgtgg acgagcgcga cctcgaccgc gcgcaggaaa tctacgtctc
     4021 tttcttcggt caggacacca cgccgctcac gggggcgacc gccgaggaag aaacgacaag
     4081 tgatgagtga taagtgatga gtgataagaa agaacggggg ctgttagctc ttagcgatta
     4141 gccaacagct aacagcttct catcacttat cactcatcac ttatcactac tgcggaggtt
     4201 cggcatggac gagactgatt cgggggccat tatggtggtg atgaccgcgg ggagtcgcga
     4261 ggaggcttcg cgtctggcag agatgctcgt cggctctcac ctcgccgcgt gcgtccagat
     4321 tctgccggag atcgaatcgg tctatcgctg ggaagggaag gtgcaccgtg agccggaggt
     4381 gctgctcttc gccaagacga cgcgggcctg cttcgcggag ctggagcgtg aggtgcgcgc
     4441 cctgcacacc tacgacacgc cggaaatcat cgcgctgccc gtcgccatcg gctccgcgcc
     4501 ctacctcgaa tggctcaacg acgtggtgat gacgccctac ggggcgcgcg ccgccgagcg
     4561 acacgccgag tcgcagggtg aggcgacgga tcagtagtcg taggcgctcg cgtcgtcgag
     4621 ctggcgcaga gagttctggc tctcgacgac gatacgcaca gcctcctccg gcgaatccgt
     4681 gaggtgcagg agattcaggt cggtctccga aatcatcccc tccgcaagca gcacttcctt
     4741 cgtccacctc agcaacaact cccagtactg tgagccgtag agcacgacgg ggaagttcct
     4801 gatcttgcgc gtctggatga gcgtgagcgc ctcgaacaac tcgtcgagcg tgccgaagcc
     4861 gccggggaag atgatgaagg cggtgctgta cttgatgaac atcgtcttgc ggacaaagaa
     4921 gtagtcgaag gtgatggagc gcgtctggta ggggttgggg cgctgttcga agggcagctc
     4981 gatgttgcag ccgatggaga gtccaccggc gtcgagcgcg ccgcggttgg cggcctccat
     5041 gatgccgggg ccgccgccgg tgatgacggc gaagccctcg cgggcgagga gcgccgccgt
     5101 ctcgcgcgcc gcctcgtaca tggggctgtc ggccggcgtg cgcgccgagc cgaagagcga
     5161 cacgccgcgc gtgacggtcg ccagctcgtc gaacccttcg acgaactcgc ccatgatgcg
     5221 gaagacacgc cacgtgtccg aacgcttgaa ctcgtccgcc cgcggcgact ccagcaactg
     5281 ctcgtcctgc gtcaccgccc gcccggccgc ctccgtcttg tgctttgcct tcgtgtgagc
     5341 cataaaaaaa gaggtcagag gttagaggtc agaggttagt ggaagacagt tttttactga
     5401 cctctaacct ctgacctctg acctctgttt tcagaatgcc atgatgttgt aaccgcaatc
     5461 gacgtggagc acctcgccgg tgatgccgga ggagaggggg ctgaggagga agagcgcggc
     5521 gttgccaacc tcgcgggcct cgacgttgcg tttgaggggc gagtgctcgg cgtggtgctt
     5581 gagcatcgtc gagaggttgc cgacgccgcg ggcggcgagg gtgttgatgg ggccggcgct
     5641 gacggcgttg acgcgaatgt tgcgggggcc gaggtcagag gcgaggtagc gcgtgctcgc
     5701 ttccagcgcg gccttcgcca cgcccatgac gttgtaatgt gggacgacct tctcggcgcc
     5761 gtagtaggtc atcgagatga tgctcccgcc ctcggtcatc aggggagcgg cggcgcgggc
     5821 gagcgtgacg agcgagtagg cgctgatgtc gagcgccatg agaaaggcgt cgtgcgtggt
     5881 ctcgacgtac tcgccttcga gcgcctcgcg gggcgcaaag gcgaggctgt ggacgaggaa
     5941 gtcgagccgc ccgaaacgct cgcgcaccac actgaacgcc tgttcgacct cttcgggctt
     6001 cgtcacgtca cactggatga gcggcgcatc tgtcccggcc gtcaagctct ccacgttctc
     6061 cttcaaacgc tcgctctggt agttgaacgc gaggcgcgcg ccggcctccg tgcatgcctg
     6121 cgccgtcgcc cacgcgatgc tgcgcttgtt ggcgacgccg aagacgatcc cgaatttgtt
     6181 ctcaagcagc attgaaagtt tcctcgaatg gattcattcc cgctccagtt ccttgagctg
     6241 cgccttcaac tcggcagtgc gcgcacgggt cacgtccgcg agacagaacg agtgaatcat
     6301 cggccgcatg gagccgccct cgtattcgga cgatacgaac tcgcagttgc cgtcgcggta
     6361 ctttatccaa gccagctccg cctccttcag cttcgcgcgc cggccttcgt cgagcttgga
     6421 ggagaactgg ttgtaaattc ggttgagggc ggcgtcggcg gcctgaaatt ccttgtgggc
     6481 gcagacgttc atctcgtact gggtgtactc accggggcaa gggtctttcg cggcttgccg
     6541 cttcttctgc gccggcgcgg cgacaaagag caggcacaga gcgggcagaa ggccgagcac
     6601 aactctcttc atcttcgcct cttgataaaa ccttgagcgg ttctaggttg ttttgccctt
     6661 taccatgaac gggatatgtt ggtcggcgag agcctccagc gcagccgcgg gaatcaggtg
     6721 ctcacctgag ttgagcacat ggccggagaa gcgcccgatc agaaaggcta aggcgcgctt
     6781 ctccgtttcg cggtcaggaa atgtaatggt caccatgtct cacacacctc ggcgcactgc
     6841 gcccatcacg cggcggcgct cgccggctgg cccgacatca cgcgctggta ctcgccgctc
     6901 tccacccact tgagaatctc ggagacgcgc cagacgggga aggggtgcgt caagccggca
     6961 ttgatgatgt cggcccagaa gcggtcgagc gccttctggt cgtagttctt ctggaagtcg
     7021 cgcgcctgtt cgagaaactg atcgtaatcg acgcgcgagg cgaaggtgcc gccgcagagt
     7081 ttcatcatgg cgcggccgat cacgtccggg tcttgcacga cgagcagcgc cgcgcggtcg
     7141 cacgaaagct cggcgcgacg cgcccacgcg aggatggccg cgcgcatcgt gcccgagatg
     7201 agctgcgcga tgatgccggc gaggggcgtg gtggagatgg ccgcgttggc gagcagttcc
     7261 agcagatgcg ccgccgtcag gtaaaggacg tgctcggcgt ggatgtggcc gacctcgtgg
     7321 gcgagcaccg ccagcgtctc ctcgtcgttg aggcgttcga tgagcgcgga gtggaggaca
     7381 atgacggggc gcttgacgcc gccggtgaag gcgttgacga cggggttctg ctggacgaac
     7441 agctccggca tgtccacgcc gagcgtggag caggcgatct ggagcttggc gtagaggtcg
     7501 gggcactgct tcggcgtgac cttgacggcg ctcgccgtga agatgatgcg gatggccgac
     7561 tcgccggtca cttccaaaag cttcttgagc gccgagtcga tgccggggat ggtcttgagg
     7621 gcggcaaggg cgcggctgtc ggccgggtgg atgtactcgt gcttgtcgag ttcggcgaat
     7681 tttttgtggg gctcgcctga gagcgtcagg cggcagcggc cgcaccgcgc ctgagcgttg
     7741 ctgttgtcgc gcgtctggtt caactgcccg cagtagcggc agcggatggt gtcgttgttc
     7801 tgttcgttca caggttggat gtcagacatc tacttctcca taccgaaatc aaagacgtga
     7861 caggtgctgc tggagagaca agttcgcaag cctgttcgga gcgttaagta tccgtgatgg
     7921 acgggcgcga gggcaagcgg acaggagctg ttagctatta gctgttagct ttcagctatt
     7981 agcttcagtg cctgaagtgg cgcaggcccg tgaagaccat cgcgaggccg tgctcgtcgg
     8041 cggcggcgat gacctcgtcg tcgcggacgg agccgccggg ttggatgacg gccgtgacgc
     8101 cgtgacgggc cgcttcgtcg agaccgtcgc ggaaggggaa gaaggcgtcg gaggcgacga
     8161 cggagccggc cagcgtgagt tgcgcgcgca tggcgccgag gcgcgcggaa tcgacgcgcg
     8221 acatctgccc cgcgccgacg cccacggtct gaccctcgcg cgcgtagacg atggcgttcg
     8281 acttcgtgtg cttgcagacc gtccaggcga agaggagcgc cctcgtctct tcctcggtcg
     8341 gggcgcgtct ggtaacgacc ttgagctggt cgggcgcgag ccgatgagcg tcgcgcgtct
     8401 ggatgaggat gccgcccgtg atgcgtttga agtcgaggcc ctcgggcggc tcggccgggc
     8461 cggcgcgcag cacgcgcagg ttctttttcg acttcaacac ttccagcgcg ccgtcctcgt
     8521 agtcgggcgc gacgacgacc tcggtgaaaa tctctgtcag ggcgcgcgcc gccgtctcgt
     8581 cgagagtgcg gttgaaagcg acgacgccgc cgaaggcgga gaccgggtcg gtggcgagcg
     8641 cgagcctgta ggccgcctcg gtggtcgcgg ccgtgccgac gcccgcgggg ttagtgtgct
     8701 tgatgatggc gcaggccgtc tcctcgaagt cgcagacgag ttgccatgcg gcgtcggcgt
     8761 ccacgtagtt gttgtaagac atctccttgc cgccgaggac ttcagcggcg gagacgcctc
     8821 cgctctcgct agtgacaaag agcgctgcct tttggtgcgg gttctcgccg tagcgtagat
     8881 cagttttctt ctcgcagagc agagtgatgt ttttcgggaa tgatccctgg gcttcgacgc
     8941 gcaactcgac agcttgtagc ttgtcatagt ccgtgacggt aaagtcctca ctcatgcatt
     9001 gaacgtcctc cgagatcagg tctccggtct gcatcctgtt ttttgccagg ccgggcagcg
     9061 ttgaagcgag gaagctgaaa atggcacggt catactccgc tgtaagcatg aatgcctgtc
     9121 gggcgaggcg ggcacgcgtc aggagggaga gcgaccctcg ttgcgctttc agttcatcca
     9181 cgattttctc gtaagagtag ggggacgaaa cgacagccac gtcgccataa tttttggcgg
     9241 cggagcgaat catcgccggg ccgccgatgt cgatctgctc gacggcctcc gcgagcgtga
     9301 cgccctcgcg ctcgaccgtc tgctcgaagg ggtagaggct gacgacgacc atgtcgattg
     9361 gctcgatgcc gtgctcgcgc atggaggcgg cgtgctcggg gttcgcgcgc acgccgagca
     9421 ggccgccgtg gacgcgcggg tggagcgtct tcacgcgccc gtcgagcatt tcggggaagc
     9481 cggtcaagtc cgacacgtcg cgcacctcga cgccggcctc gcgcaaggcg cgcgccgtgc
     9541 cgcccgtgct taaaatctcg acgccgtgac cgcgcagagc gcgcgcgaag tcgatcagtc
     9601 ccgccttgtc cgagacgctc aggagcgctc gtcgaatcgg cttcaagccg accgtgttcg
     9661 gttcagtcat aagttcgtgt cgccttccat tgtccgacta agaaatattc agagcacgtt
     9721 atcaaaacta tatctggggc cgggcaagtc ctcagcccgt catggcgaaa gaattattaa
     9781 catggatgga taggatgtgc gggatgagaa gagttgaaag ctcaaatctg ctttgtcttc
     9841 atcctatcca tcctgcccat ccatgttgat tcttctataa gcaggggccg cgctcaaggc
     9901 tccggcgtgg cgctcggggg tggagcgccg ccgccttcgg gtcggcgttc gagaacgtat
     9961 gtgtcgcgga gcgcgcggac acggagtttg cccgcgtccc gtgtcttgag acgcacttcg
    10021 acgcggcgcg ggaggccggt gcgctgcgct tcacccgcgt agtagccgag gacgtactga
    10081 gcgcggaggt cgtcttcgat ggcgcggaag acgggccaga agtcgtgcgg cgtttgtgcg
    10141 ccggaagatg tttccgcgtc gccgacgacg aagagttgac cgcccgtctt tagagcgagg
    10201 tcgcggaagc ccttcgcggt cgggcgcggc ccgagacggc cgccctgaat ccagaagagc
    10261 ggcgtgtgga tcacgtagaa ggcgacgccc cgcgcgcgcg cctcgtcgat gacggccgag
    10321 gggcggacgc ggctggccgt gtcgaggccg tcgctgatga ggatgacgat gcggcgctcg
    10381 gccgggtcgg cgccgggggt gtcgaagacg cggacggcgg cgagagcggc gtcgaagatg
    10441 gccgtgcggc ggtcgggtag agcgacgaag cggaaggcgg cgcgcgccgc ttcgaggtcg
    10501 gcggtgaagg ggagcgacag agcgggccgc tcctgaaagt ggatgacgga gacgcgcgaa
    10561 cgcttgccga agcgcgagaa gagggcgagg gcagcctcgc gctggcccgc gatgatgtcg
    10621 cgcgtgctgc ccgaggcgtc gaggaggaag acgagagcca cgcgctcggc gccgacggcg
    10681 aagtaggaaa tctccacgcc gctgccctcg tcgatgattt caaagtcggc ggcggtcagg
    10741 ccagcgacgc gccgtgcgcg ccggtcggtg atgatgacgg ggacgctgac gaggtcggtg
    10801 ttgacgcgaa tcgtttcgtc cggcggggcg gcgtcggtct gcgcgcgtgc gtgtaggtgt
    10861 gcggagacga gggcggcgaa gagtgtggcc gtgacgaata ggaggcgggc ggagcgcggc
    10921 gaatcggcaa tctgtgggtt cgctgcgccg ttggaggcca tgccgtcaga gggcttagaa
    10981 caggaagcgc ccgaagaaga gaatgctcag gatgacggca atcgtgatga cgatgatttt
    11041 gacgaggctc acaaccttgg tcctttcggt gtgcgaggca cgcggggaaa tccaacgcgt
    11101 gtgcggcatt ctatcgacgc ccgtcgtggc gtgtcaatgc cggcgtcggc ggctgcccga
    11161 gtcgaaaact accggcgtct gtgcaaaagt agatacgccg ccgaagtgtt gcttgttcct
    11221 cggcagttcg agccatccgt gcgcggcccc gagacatcaa agaagggcgt ttcaatcggc
    11281 ggttcttatt gctataattc cggcactttt gtaaggtttt cacaactcag gaagttcacg
    11341 attgcgcgga agaggtctca gaggcaattt cggcaggtgg tcgcccgcgg cgtccgtctt
    11401 gctgctcgtc ctgttcgcgc acgccttcct cgtcaacgcc acgcactgcc atcgcttcgc
    11461 gcgagccgtc gcgccgggcg cggagcagga aatcggcgtg agcggccggg aagacctcgg
    11521 tcgccagagc gaggccggcg gagacgcgca gtgcgtgctg tgtcgcctcc agcgcaactt
    11581 cgtcacggac ttccacaggg tttcgacgcc ggtcggccca acgcggcagg agtgtcaacc
    11641 ttaccgtctg ccgccggccc tctccagcaa cgcgcgcgcg ttctccgtcc cgcagggccg
    11701 cgcgcctccg ctcgcctgaa tactccaacc tacgtatcag tcccggctcg cctgtcgcga
    11761 cgctgaaaga taactcgaac tcgaagccgc atggccgaag gggagacgca gtcgcccggt
    11821 gtggtggctg cgtccgaaac gctctgcttt cgagtggggc gctctccaca aaagagttat
    11881 ttcgcgtgtc gccacccgga cgttgaaaca tcagttcggt gcttttcatt caggagtcat
    11941 atgaagcgga aaatatctct cacatgcttg ttggccgcgg tcgtcttgtt attcgccccg
    12001 gcccgcgcgc tcgcacagat gggcggaagt ctctacagcc tcccgcagac caacggcgcc
    12061 atcaagggga cggtgacgct tgccgagggc ggcacggccc tgcataacgc catcgtcacg
    12121 gtcgtgcaac tgaggcgttc ggttcagacg gacgagaacg gggcttacga gttcacgccc
    12181 gtaccgcccg gcgcctacac ggtgctcgcg cacatggaag gtttcccgga cgccacggag
    12241 acggtgacgg tcgcggccgg cgcgcccgcc acactcgaca tcaagttgag tctctccggc
    12301 ctccgcgagg agattacggt gacggcgacc ggcaccgagc agagcacctt cgagtcgctt
    12361 caatcggtca ccacgctcga caccatcagg ctcacggagg agagtcaccc gtccatcggc
    12421 gaggtgctcg acaaagagcc gggcgtggcg aaacgtagtt tcgggccggg ttcctcgcgc
    12481 cccgtcattc gcggcttcga cggcgaccgc gtgctcatca cgcaggacgg cgcatccacg
    12541 ggttcgctcg cctcgcagtc ggccgaccac gccgagccga ttgacgtgct ctcgctcgaa
    12601 cgactggagg tcgtcaaagg cccggccaca ttgctctacg gcagcagcgc cgtcggcggc
    12661 gtcgtcaacg ccgtgacggg ccacgactac gcgcacgacg gctggcgcgg ctatctaacg
    12721 ggcgtcggcg gcacgacgaa caatcagggc ggcgcgagcg gcgggctgga gtacggcacg
    12781 ggaaagtgga tgctctgggg caacggctcc gcgcagcgca cgggcgacta caacacgccg
    12841 ctcgggcgcg tcgagcaatc gttctcacgc tccggcagcg gcacgcttgg tgtcgggaga
    12901 tacgaagaga agaacttcct caatttcaca tatggttacg accgtcgccg ctacggcatc
    12961 ccgttcgctt cgttcttcga gaccggcggc gaggagaccg gctcggacgt gagcatcaag
    13021 acgaagcggc aagacttcaa gttcaacggc ggcttccgcg acctcgacac gcctgtcagc
    13081 ggcttccgca tgactgtcga ttacagcgac tacgggcata gggagcttga gggcgaagaa
    13141 gtcaacacac agttcagcaa caagctgttc atgtaccgcg gcgtcttcga ccagcgcaag
    13201 cgtggaagcc tttcgggcag cttcggcttt tccggcttcc accgcgacta cgacacggtc
    13261 ggcgcggaag cgctggcgcc gcccaccaca cagaacaact tcgccgtctt cggccttgag
    13321 gtcatcgact tcgagcgcgt caaattccag ttcggcgggc gcgtcgagca caactcctac
    13381 aaccccgacg gcctgctcgc gcgctcctac accggcttct cgggcgcggc cggcgtccgc
    13441 gtccagctct ggacgaacgg cgctttcgtc gccaactaca cgcactcgta ccgcgcgccc
    13501 gccctcgaag agctttacaa caacggcccg cacgtcggca acctgacctt cgagatcggc
    13561 aacccgctgc tcacgcgcga gagcaacgac ggcgttgatt tctctctgcg ccacgagtcg
    13621 aaacgcgtcc gcggcgcggc gaacttctac tactacaaca tcaaggactt cgtcttcctc
    13681 gcgcccacgg gtgccatcga ggacaacctc atcgaggcgc gctacgggca ggaggatagc
    13741 cgattcatgg ggacggagct gaaccttgag ttggcccccc gcgagaacct gtggctcact
    13801 ttcggcttcg acgccgttga cgcgcagttg aaggcgtcgg acacaccgct gccgcgtatc
    13861 ccgccgatgc gcgctcgctt cggcatcgac tggcgctaca agggcttgag cctgcgcccc
    13921 gaggcggtct tcgtaaaaga tcaggatcaa ctgttcccga ccgagacgcg cacgcccggc
    13981 tacgcactct tcgacgtggt cgcctcctac accctcgcgc gcccccacta cgcgcaaatc
    14041 ttcgccgtca acgccttcaa cctcggcgac cgtctctacc gcaaccacct ctccttcatc
    14101 aaagacctcg cgccggagat cgggagaggg gtgcgcttca cctacactct caggtacttc
    14161 tagggcgatt gtcacttgag ggaaaacgaa atgtgattca ccccagcggc gcaaaggcaa
    14221 ggatgaagga tgagggatga ataattgctc ccacttcatc cctcatccct ccgccctcat
    14281 ccttgccgtt gcgcctccgc ggtgagtcag tctcccgaca cactcaactg ataaatgcta
    14341 taaaccgtag tcatatgccc agcaagtttg gaaaactcta tctcatacca ctccttctca
    14401 tactcggcat tacttcgtca catgcccaga cgctcgacat aaaactcagt gtcgccacgc
    14461 tcaacccgcc gcgcctgcgt gtcgagggcg cgcgcacgaa tggagcgcgg acgtggtcgt
    14521 tcctcaacgt ctacgggggc gcgctcggtc tcggcgagag gatcgaaaac ctgaccctcg
    14581 cggacgcgga aggcaaagaa gtagccgtcc gcaagcgcgc gcccggcgag ttcgaggcgg
    14641 tgcgcgaagc cacgcgcttc agctacgaaa tcaggctcaa cccgccggcc aacgcttcgg
    14701 acgccgcgca cgtctcctgg ctcacagacg aacgtggact attgatgctc gccgacgtac
    14761 tacccggccc cctccgtgac gcgagagttc gtctcgcgct cccgcccgga tggtcggttt
    14821 cgacgaccga ggtcgtgaac tctgaaggga catacgagat cgccgatgcg acgctgtccg
    14881 tcttcctcct cgggcgcgat ctccgcagca acccttcgcg ccgcggggag atggagtttg
    14941 cttctgtcat cacgggcgag tgggccttcg gcgacgacgt agcagcaagc agtgcgactg
    15001 acattctcaa ggagtacgaa gagttgatgg gcggcgtgcc cctcaggcgt gcgcgcattt
    15061 ttgtcttgcc cttcccactg gcgacacccg cgcagtcgtg gagcgccgag acgcgcggcg
    15121 gcacggtcgt catcctctcc ggacgcctgc catcgaagac gttcgcgctt tctcgtctgg
    15181 atggcatact cgcacacgag ttgctccacc tctgggtgcc gaatggactc tcgctcgacg
    15241 gcgagtacga ctggttctac gaaggcttca cgctatacga gtcgatgcga catggcctgc
    15301 ggcggaacca actcaccttt caggattacc tcaacgccat aggccgcgca tacgacggct
    15361 acaaggcggc tcgggtcgcc gacaaaatct ctctcgtcga agcctcccgc aggcgctgga
    15421 cgctcgcccc gtccttcatc tacgacaagg gaatgctcgt cgccttccta tacgacctca
    15481 cgctaacgcg gcggagcggc gggaagaatt ctttggacga cgtgtaccgg acgttgtttc
    15541 gccggcacgg cttaaagacc gcacgcgaag acgggagccg cgcggtgttg aatgccctgg
    15601 gcggtgttgg cggtatgcag gatttcgcca ggcgttacgt ggagggtcaa gccgacatcg
    15661 accttgccgc tgaactggcg ccgttcggcc tccgactcga agcgacgggg acgcgcacgc
    15721 gagtcgtcgt cagcgactcg ctggataaat ctcaaagaga cctgcttaaa aaattcggct
    15781 acaatgaaaa gagcagcccc tccccgcgga ggccgcgcga taattgagag caatctcgcc
    15841 aaatcacctt tcgtgaagcg agattggctt cagataaaga gacgaaacgt tatcaaagga
    15901 aggatgccgg cagagcaggt gccgatgatg ttacgaggtg gcttgatgag gatgctgaag
    15961 caacagtgct tgttacttct gctcgccgtc ctatccttca cgagtgggtg cggggcgttg
    16021 gccggcaagg aggagacggg cgtggtcatc gcccgtcggg cgcaggtgcg cagctctatg
    16081 gccgtggtcg ccgccgacct gaaagaggtc gtgcgtggcg acgtgctgga gattttggat
    16141 tcgatcacgg cggaaaacgg ggagcgttgg ctgcacgtgc ggacgcgcga cgccgagagc
    16201 atcgagggct gggtcgaggc gagaaacatc attccggaaa acatgatcga gcggtctcgc
    16261 cagctcgcca aggaagacca aggcgttcca ccccaggctt ccggccaact tcgcgcgaac
    16321 accaacatcc gcttcacccc cgaccggggc ggcaatgaca acatcctgat gaagttggag
    16381 agcggcgcgc gcttctacat cattggttgg aagcgtgtgg cgaaaccgaa aacctcggag
    16441 acttccgaga gtgacgacgc gccgaagtcg ggcgtcgcgc aggggaggtc ggcgcggcgc
    16501 ggcagggaag acgccgagcc aaaagagacc gaagaccccg acgagctttg gtacaaggtg
    16561 agactgcccg ccacgctttc cccggcgccg gccggctggg ttctgggcaa gcaggtagag
    16621 ttgacagtcc ccagcgacat catcttctac cgcaccggcc gggagttcgt cgcgtggcag
    16681 aggcttgacg gcgagggcga ggacgcgtcg gccaccacgc acgtcgataa ggacgccgcg
    16741 aaagagacga gacccggcag ttgggtgatt ctggagaaga gcagtacgcg tgataagact
    16801 gaaaccgacg ccccggagtt tgatcgcatc ttcgtcctcg gttacgacaa ggatcgacaa
    16861 gagcattaca ccgcctaccg cagcccagac ctcgatggtc tcctgccgct cagagtagaa
    16921 ggacagggtg ataacaaaat cttcaccgtt aagatcaggg acaacggaca aacaagagac
    16981 gttaggttca aagcatacat cgactccaga gggctgctca aagtcgagtc gctggacgag
    17041 ttgccgaaga ttcaaaaggg caggtaaagg ggcgcgatca gcgggcctca ttgccgcttg
    17101 atggttgaaa tttggatggt ttcacgggcc acacattttt cttgcgaggg cagttgacaa
    17161 attattgatt atgcttataa tgcccattcg cctgaaacac ggcggttgac atctcagaaa
    17221 ttcaaaagcg ctttaccaag ccgcttctct ttcaaagcct ttacgcgaga gggagcggct
    17281 gtttcatcgg caggcaaccg gacggccttt gaagcatcaa aactcgaccc atgcttgaca
    17341 agcaagtagg gctgcggtaa agtcaccgtt tccttgagac gaaaaataat cgtccttagg
    17401 gcaaccggaa gaatcctgag acatctaaat ccggattcgc tgaagttgag tgaaagcgat
    17461 ctttgaaaac tagatagagc gtgtcccaat aggatgacct ttgagagcga cgtggacgat
    17521 aaagtccgcg tactcaaaac tcgaaagagt catcatcgga caatgtacta accagtacga
    17581 tgatgttcca gagaacaaat tctaacgaga gtttgatcct ggctcagaat caacgctggc
    17641 ggcgtgcctc agacatgcaa gtcgtacgcg aaatctccct tcggggagaa agtagagtgg
    17701 cgcacgggtg agtaacacgt gggtaatctg cctttgggtg gggaataacg accggaaacg
    17761 gtcgctaata ccgcataacg cagcggcacc ttcgggtgac agttgttaaa gcgggggacg
    17821 ggctttcggg cccacctcgc gcctaaagag gagcccgcgg cggattagct agtcggtagg
    17881 gtaacggcct accgaggctt cgatccgtag ccggcctgag agggcggtcg gccacactga
    17941 cactgacaca cgggtcagac tcctacggga ggcagcagtc gggaattttg ggcaatgggc
    18001 gcaagcctga cccagcaacg ccgcgtgaag gatgaagcat ttcggtgtgt aaacttcata
    18061 agagtgggaa gaataagtgg gggctaatac ccttcatgat gacggtacca cttgtaagcc
    18121 ccggctaact ccgtgccagc agccgcggta atacgggggg agcaagcgtt gttcggattt
    18181 actgggcgta aagggcgcgt aggcggccac cgcaagtcga ctgtgaaatc tccgggctta
    18241 acccggaaag gtcagcc
//
LOCUS       DASVES010000285        18180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_653126, whole genome shotgun sequence.
ACCESSION   DASVES010000285 DASVES010000000
VERSION     DASVES010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18180
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653126"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VF240_11445"
     CDS             complement(<1..333)
                     /locus_tag="VF240_11445"
                     /inference="COORDINATES: protein motif:HMM:NF022766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HEX7175863.1"
                     /translation="MLKLALVTLYLLALTACASRQAERQDASQPAPVRTESNVVTGGE
                     GTPIVGQTIYVPVYSHIYARDKSRVINLSATLSIRNTDAQNPISIASVRYYGTNGALV
                     REYLNKAIS"
     gene            complement(401..1930)
                     /locus_tag="VF240_11450"
     CDS             complement(401..1930)
                     /locus_tag="VF240_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX7175864.1"
                     /translation="MSTTLQRERKEQASVPPVRELADQAFSRAAGAPLVAGNSVRLLI
                     DARENYPAWLEAIGAAERYIHFESYIIHEDETGWMFADALTAKAREGVRVRLIYDWMG
                     GFGKTSRKFWNHLRAGGVEVRCYNPPRLDSPFGWLSRDHRKMLGVDGQVGFITGLCVG
                     RMWVGVPEKRIEPWRDTGVEVRGPAVADIEQAFAQVWAMMGEPIPEQELTDRGAPAPA
                     GDMRLRVVASVPSTAGMLRLDQLVAALARNRLWLTDAYYAGTTSYVQALRAAARDGID
                     VRLLVPNGTDIPLLRPLSRAGYRPLLEAGVRVFEWNGTMLHAKTAVADSRWARVGSTN
                     LNIASWFGNCELDAVVEDESFARLMEETYLQDLENSTEVVLDAKRKVRAPGEPPHPHP
                     VMTSGGGSGGRAAAGAIRIGNAIGAAFTDRRVLEPVEARIMVTAGALLLALAILFWFF
                     PRLLVYPVTVILLWIALSLLYRGYKLHRRKGKQETGALDERAAPHRVAEAPQEIESKR
                     E"
     gene            complement(2089..2574)
                     /locus_tag="VF240_11455"
     CDS             complement(2089..2574)
                     /locus_tag="VF240_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175865.1"
                     /translation="MSSARETEPTSRSSGNSIWQNERVRRLILYAAVLLVVFLVGLVP
                     MWMTARERARERDAAQAALRISTLQNTLANAAIDARRGEYEPARQAASDFFTNLRAEI
                     ERGRDSVFNETQQNTLRSIFIDTRDDTITLLARSDPASADRLADLYNKYRQAVGGAPP
                     R"
     gene            complement(2599..4218)
                     /locus_tag="VF240_11460"
     CDS             complement(2599..4218)
                     /locus_tag="VF240_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7175866.1"
                     /translation="MKFKLLLLASFACLLIPVAAAARQQAPELLTYDELVQLYEQETP
                     PEALQNKLRLLLTTPFVSNAATARGVRPLLPSTAKLGQLLRVALWNIERGLEYEAIRL
                     AFTDAAAFARLIDSSEYPRGSEKRGLILQQVQLLKQADVIVLNEVDFGMRRTDYRNVA
                     EDLASALGMNYAYGVEFVEVDPIALGLEKFEELPPEDAAKLIAEISVNRGRYRGLHGN
                     AILSRFPLENVRLEPFEQQPHDWYKDELESVRPLEAGKRKAGELVFQEKIRREVRRGG
                     RMMLTAEIADERIPGGRLVVVATHLEDKTKPEGRRKQLEELLARIKDTAGTVVVAGDM
                     NTSTQDGTPLSAERLLKKRLGSRDFWIKQGLGILTGIKLPTMLLGGLNTYRKQADPTV
                     RSIPLVASNPEAGFFDRLKDFRFADGGAFDFRGERGRSVGEKDDTLANSNQRGDKGFI
                     TTYEVERTVAFVGKFKLDWIFVKPFALTEPDDDEQPHRFAPHFGRTLKSLNDALTDRI
                     SDHAPMMVDLPLDEPRPARGKRRRAVATKGN"
     gene            complement(4633..5865)
                     /locus_tag="VF240_11465"
     CDS             complement(4633..5865)
                     /locus_tag="VF240_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7175867.1"
                     /translation="MDNIILEIGLALVLIAFAVVLAHRLGLSNIPFLIIIGMAVGPHA
                     PQFGVFDFRFIETGSLIEYMGRIGVLFLLFYLGLESNVSRLIEAGRSILVGGTIYVSI
                     NFTVGFTFGYLAGFPLREALIIAGLTTVSSSAIVAKVLFDYRRMANPETQIVLGITMF
                     EDVFLAVYLSLISGIVLSGATSLGGVLSSGGIALGFIIGLMVAGRWATPLLNRLLSIS
                     SNEVFVVVVFACLFLLAGLGETIHVAESIGALLLGLILGETEHSERMERLVVPFRDFF
                     GAIFFFGFGLSIDPFTLGGAAWLATGAALLSLVGVIIAGLIVGRRAKLSTVGSLNTGF
                     TLLARGEFSIIIVNLALAGGLMAVLQPFAALYVLILASASPLLAKESERIYALFAKLF
                     GRLGKMKTPDEEVEEAQV"
     gene            complement(5870..6370)
                     /locus_tag="VF240_11470"
     CDS             complement(5870..6370)
                     /locus_tag="VF240_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX7175868.1"
                     /translation="MSTISEAFLPGVGRKFQVETISGDRLVVVIHDDGTRELYHFTRR
                     NLERPASVLRLSDGEARQIAGIIGGLTYVPRSLPMAEVILGDLVLEWFTIEKGAACVG
                     RTIRDLQVRTATGASIVSILEADQTKRLNPEADTVLNEGATLILAGDRSTINAVKRLL
                     VEGRAG"
     gene            complement(6566..6961)
                     /locus_tag="VF240_11475"
     CDS             complement(6566..6961)
                     /locus_tag="VF240_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175869.1"
                     /translation="MLEIAVGTLSLFFGWLCFDHVSRVRALYSKDRSTRSRSYGEKPL
                     TIFHFALGVLFVLDGLALLALAAYERLHVADGLENIVDLEIMLIPAIVFITTVALMRT
                     ATGSRRDKQKAEAAEVRAGDDADLFVAGK"
     gene            complement(7177..8148)
                     /locus_tag="VF240_11480"
     CDS             complement(7177..8148)
                     /locus_tag="VF240_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7175870.1"
                     /translation="MKVLIAYDGSAYADAALDDLRRAGLPREAEALIVSVGDGLVSTS
                     TPMAEVAGTALTARRVTSAIAVAKEQAARLLAEARGFATEASRRVRSYFPGWEVRAEV
                     LEGSPSRELLRKAEEWRPDLVVVGSQGRSALGRFFLGSVSKALATNARCSVRVGRRGV
                     EKNDVAPPRIIVGVDGSRGAARAVRAVGMRAWPDGTEVRLIVVDDGSGPTRITDIKPR
                     LEELYTGCNEGPPVNAHLMAEGARVVLLDEGLDASVVIREGDPRRVLIDEAREWAADS
                     IFVGSHGLDHPDERSGLGGVSAALVTNATCSVEVVRSGLLIKIEGGE"
     gene            complement(8367..8684)
                     /locus_tag="VF240_11485"
     CDS             complement(8367..8684)
                     /locus_tag="VF240_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175871.1"
                     /translation="MSRRINTPGGRKGRRGSLIRVLSAAAFVSALLYWEQTALLYVLS
                     TLAMCGLLLVVAFSDLEGRDKELTESTLNERAAVADDNKLTTTPSLDQARGATKRKRQ
                     DAA"
     gene            complement(9209..9853)
                     /locus_tag="VF240_11490"
     CDS             complement(9209..9853)
                     /locus_tag="VF240_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7175872.1"
                     /translation="MAKRLLVVDDEAKLLRAVAVTLREEGYEVTTARSGAEALVSIQR
                     GVPDLIISDIRMPGMDGYQLARALRSNPRTELIPIIFLTAKGERKDRIAGLRTGVDAY
                     LTKPFDPEELLAVVSNLLSRAERTGAELARLVSTAKGEESSPSHYAPDEDFTDAEARV
                     ARLVADGLSNKEIAAELGLSVRTVEGHISNILSKKGWSNRVEIARHVLGRGASG"
     gene            complement(9846..11054)
                     /locus_tag="VF240_11495"
     CDS             complement(9846..11054)
                     /locus_tag="VF240_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7175873.1"
                     /translation="MSALGENTGKKLRRYLIAVWSVGAVFVLTMFATRYVEMDVSSAY
                     LAAVMFSAWRGGLGAGLLATALSAVVGAFFFFPPAYSFKVNGEDLLELGVFTLAALII
                     SSLSAARERALALEQQARREAEAANVVKDEFLAAVSHELRTPLTTIKTLTRVLLRKNP
                     TEEERREYLEDIASECERQIDLVHNLLDLSRVKVGGVEIRAARVDVGEALRACEKIER
                     AAAVERNQELVVGPVSDLPPVRADKSALRRALCAVVENAIKYTPDGGRIILRARRDED
                     GGRVAIEVEDTGVGIRTEDLPRIFDRFYRGRAGAGDGAGHLDEQEVPGIGLGLHLASV
                     LVEGMGGSIEASSRVGRGSTFTLRLPVWRDETDDEANVDEGGWATSSVKAPAESLASG
                     AVKREGLSNG"
     gene            complement(11051..11284)
                     /locus_tag="VF240_11500"
     CDS             complement(11051..11284)
                     /locus_tag="VF240_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175874.1"
                     /translation="MDSKSKQKKQREGLPTGAGGEDRIEAKIDRDLQDSFPASDPPGW
                     VLGVKRRKAEKEEEDAGGSSTAATGDAGGEKVS"
     gene            complement(11454..12167)
                     /locus_tag="VF240_11505"
     CDS             complement(11454..12167)
                     /locus_tag="VF240_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7175875.1"
                     /translation="MPKSARPPTPQNKILAALPTKEYNRLLPHLKLVSMPVGETLYEM
                     EGRIKYVYFLNGAVVSLVTHMEGGTSVEVGLVGNEGMVGLSVVMGDDVSQNHAIVQIP
                     DGAMRMGTGKLKEELKRGGKLQSLLLRYSLALLKQVSQSAACNRNHNLGERLARWLLL
                     CQDRVGGVELRLTQEFLAQTLGTRRSRVSEAAIILQNAGLIRYSRGVITILDRGGLEE
                     FVCECYQVVKAESDRLLSW"
     gene            12601..13560
                     /locus_tag="VF240_11510"
     CDS             12601..13560
                     /locus_tag="VF240_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7175876.1"
                     /translation="MIKVRTAGGRGKALTKGGDGRRTFSQDRILVSVKDGTSKPLFPS
                     DEPSLTSTACAWGGLLVEHHRLPPAEFPERVATSHLVALHLRPAKLEWFLGGHPQTRR
                     MTRGSIDIVPQGTLLGGYSRDETEFLMMALDPSFVERVASEAGVAIPIELVRHLGIRD
                     PQIEHIGLALKAELETGCLSGRLYGESLAVALAAHLLGKYAANVPKTHSDNAALPAYK
                     LRRVIEYINDNLTEDLTLAEIARVAGMNPHYFSRAFRQSTGIPPHRYVINCRVEKAKR
                     LLADDELPLVEVGLTVGFQNQSHFTTLFHKLTGVTPKAYRDET"
     gene            13861..14427
                     /locus_tag="VF240_11515"
     CDS             13861..14427
                     /locus_tag="VF240_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7175877.1"
                     /translation="MDGALVKEGGVSCLVLPVGARDHAQGSMEAAVTLLEYGDFECEH
                     CAEAFPVIMKIKRWLHGALCYVFRHSPAAEKHPHALRAAEAAEAAGAQGRFWEMHDLL
                     CERSPAIDDVHLKRYAQELGLDLERFEREMRERVHLGRVREDFESGVASGVSDTPAFF
                     INGVRYEGEADLDSLLAAIEEAGTSSHG"
     gene            14893..15822
                     /locus_tag="VF240_11520"
     CDS             14893..15822
                     /locus_tag="VF240_11520"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7175878.1"
                     /translation="MASGEVTESGGTWNRAVQSITYVSSADNTRQPMMFYKPETDEPR
                     PLLVALHSWSEDYRQAESVIYSEWCVANDWVFIHPNFRGANTRPQATGSELVIGDVLS
                     AIDYAKANARVDESRIYAVGWSGGGYLGLLLAGRQPEIWAGVSAWVPISDLNAWYEES
                     RRLGTKYVSQIAASCGGRPTGETPAAAECRKRSALTYLERARGVPIDINHGIRDGRNN
                     DDPVPIGQSLRAFNLLAAPQDRFTEEEVAYFTREAQVPPALRSETSDPSYGGLRILFR
                     RQSGNARVTVFDGAHDKNTEAAFRWLNRHRRDR"
     gene            15840..16700
                     /locus_tag="VF240_11525"
     CDS             15840..16700
                     /locus_tag="VF240_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEX7175879.1"
                     /translation="MFELFKSIIPYITPIFVISTMLNVGLTQKLSDVIGHLKNYPFVL
                     KMLVANFIVAPLVMLSMLYVAPFEPTLKGGLLIFSMCAGAPFLIKLTQTAEQNLALGA
                     AVMMLLIVGTVVYVPIVLPLILSDLTVDAWAVAKSLTTQMLLPIVVGMLAVQFLPALS
                     RRVQPWVGRLGNITLYALIAATLIGYLPGMFGIVGTGAILVGLAFVLAAFGLGYLTGA
                     GKDHLEDVGGLGTAQRNTAAATIIAVQNFSDYPDVLVIITLANTLGIVMLMFIAKRLS
                     RDNKVEIVTV"
     gene            16814..18178
                     /locus_tag="VF240_11530"
     CDS             16814..18178
                     /locus_tag="VF240_11530"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7175880.1"
                     /translation="MSQRSPLKIVCLIILVTSAVALSPGGRTTGKAQGTAAAKERKGC
                     DKGDGGLTLPAGFCASVFADNLGRARHLTAAPNGDVYVNTWSSTYTNLKNAPGGYIVA
                     LRDTDRDGRADMVERFGPAHQDGKAGGGTGIAVHRGALYVEDIGKIVRYRLGRGRLVP
                     QGNPDVILSGLWTERGHIMHPFAITSGGVLYVNSGSITNSCQEKDRTLESPGISPCPE
                     LQLHAGIWRYDAGKTGQTFSPAERFATGTRNVVALAVNGADGALYATLHGRDNLAAHW
                     PKLYTAEQNNELPAEVFARINRGDDFGWPYCYFDPQQRKHVLAPEYGGDGGKAQGVCA
                     QKKQPDLTFPAHWAPNGMTFYRGSTFPAKYRGGAFVAFHGSHDRTPTQAGYRVAFVPF
                     EAARPAGRYEEFATGFAGAGPLAAPTEAPHRPMGLAVGPDGALYVSDDVKGRIWRITY
                     VGGV"
BASE COUNT         3514 a         5702 c         5394 g         3570 t
ORIGIN      
        1 actgatcgcc ttgttcaggt actccctaac cagcgcacca ttcgtgccgt agtagcgcac
       61 cgaggcgatg cttataggat tttgagcatc cgtgttccgg atgctgagcg tggcgcttaa
      121 gttgatcacg cggcttttat cacgggcgta gatgtgcgaa tagacgggca cgtaaatcgt
      181 ctgcccaacg atcggagtgc cctcgcctcc cgttaccacg ttggattcag tcctcacggg
      241 cgcgggttga gacgcgtctt gccgttctgc ctgccgcgac gcgcaggcag tcaaggcgag
      301 aagatatagg gtcaccaaag caagctttaa catctcattg ctccaatcat ggggcggcta
      361 ctttttcctt cgtcgcgcgt gagatcatct tccgcccggc ttactctctc ttcgactcaa
      421 tctcttgagg agcttccgca actcggtgcg gcgcagctcg ctcatcaagc gcgccggttt
      481 cctgcttacc ttttcggcgg tgcagcttgt agcctctgta aagaagcgag agggcgatcc
      541 aaagaaggat gacggtaacc ggatacacaa gcaggcgcgg gaagaaccag aagagaatcg
      601 ccaacgccag tagcagcgcg ccggcagtaa ccatgatgcg cgcctcgacg ggttcgagca
      661 cgcgccggtc ggtgaacgcc gcgccgatgg cgttgccgat gcggatcgcc ccggctgccg
      721 cgcgcccgcc gcttccaccg ccgctcgtca ttaccggatg ggggtgaggc ggctcgccgg
      781 gcgcacgcac cttccgtttc gcgtccaaaa cgacctcggt tgagttctcc aaatcttgta
      841 gataggtctc ttccatcaaa cgcgcgaaag attcgtcttc aacaacggcg tccagctcgc
      901 agttgccgaa ccaactggcg atgttgagat tcgtcgaacc cacccgcgcc caacgagagt
      961 cggcgaccgc cgtcttcgcg tgcagcatcg tgccgttcca ctcgaatact ctcactccgg
     1021 cttccagaag gggacgataa cccgccctcg ataaaggtct gaggagcggg atgtcagtcc
     1081 cgttcgggac gagcaggcgc acgtcaatgc cgtctcgcgc tgccgctctc aaagcctgca
     1141 cgtaggaggt ggttccggcg tagtaggcgt ccgtcagcca caaacgattc ctcgccagcg
     1201 ccgcgacgag ctgatccaaa cgcagcatcc ccgccgtcga aggcacgctc gccacgacgc
     1261 gcaacctcat atcaccagcc ggtgcgggcg cgcctctgtc ggttagctcc tgttcgggaa
     1321 tcggttcacc catcatcgcc cagacttgcg cgaacgcctg ctcaatgtcc gcgaccgccg
     1381 gcccgcgcac ttctactccc gtgtcgcgcc acggttcgat ccgcttttcg gggacgccga
     1441 cccacatccg cccgacgcac aagcccgtga tgaaacctac ctgcccgtca accccgagca
     1501 tcttgcggtg gtcgcgcgag agccagccga acggagaatc aaggcgcggc gggttgtaac
     1561 aacgcacttc gacgccgccc gcgcgcaaat gattccagaa cttgcgcgaa gtcttaccga
     1621 agccgcccat ccagtcgtag atgagccgca cgcgcacgcc ctcccgcgct ttggcggtca
     1681 gcgcgtcggc gaacatccat cccgtttcgt cttcgtgaat gatgtagctc tcgaagtgga
     1741 tgtatcgctc cgccgcgccg atggcttcga gccacgccgg gtaattttcg cgcgcgtcta
     1801 tgaggagacg aacgctgttg cctgcgacga ggggagcacc ggccgcccgc gaaaaggctt
     1861 ggtcggcaag ttcgcgcacg ggcggcacac tcgcctgctc ttttctttcg cgttgtaagg
     1921 tggtgctcat cgcctcccct gagcacgaag tcaactgaat aggagcaaag gcgaacgctc
     1981 acctgagcgt tcgccttacc gttactggaa ccggaagcct tactgccttc ggtctgccga
     2041 atcaccagcc tatgaggcag ctcgacaact cacgcccggc gccccacctt aacgtggcgg
     2101 cgcgccccct accgcctgac ggtacttgtt atagaggtca gccagccggt cggcggaggc
     2161 cgggtcgctg cgcgccagca gcgtgatcgt gtcgtcgcgc gtgtcaataa atatcgaacg
     2221 cagagtgttt tgttgcgtct cgttgaagac tgaatcgcgc ccgcgctcga tctccgctcg
     2281 caagttcgtg aagaaatcgc tggcggcttg gcgggcaggc tcatactcgc cgcgtctggc
     2341 gtcaatcgcg gcgttcgcca aagtgttctg caaggtgctg atgcgtagcg cggcttgcgc
     2401 cgcgtcacgc tcgcgggcgc gctcacgcgc cgtcatccac atggggacga gtccgaccag
     2461 aaagaccacg agcaacaccg cggcgtagag tatcaaccgc ctgacgcgct cgttctgcca
     2521 gatggaattg ccgctgctcc ggctcgtcgg ttcagtctct cttgcgctac tcatgtagtt
     2581 tctcctcgaa tgaaatgttt aattcccttt cgttgctacg gcgcgccttc gcttacctct
     2641 agcgggccgc ggctcatcga gcggcaggtc aaccatcatg ggcgcgtggt cggagatgcg
     2701 atccgtcagg gcgtcgttta ggctcttcag cgtccgcccg aagtgcgggg cgaagcggtg
     2761 cggctgttca tcgtcgtcgg gttccgtgag cgcgaatggt ttgacgaaaa tccagtcgag
     2821 tttgaacttg ccgacgaacg ccactgtgcg ctccacctcg tatgtcgtaa tgaacccctt
     2881 atcgccccgc tggttcgagt tcgccaaggt gtcgtccttt tcgccgacgg agcgtccgcg
     2941 ctcgccgcga aaatcgaagg cgcccccgtc cgcgaagcgg aagtctttca ggcggtcgaa
     3001 gaacccggct tcggggttag aggcgacgag cgggatgctg cgcacggtcg ggtcggcctg
     3061 cttgcggtag gtgttcaagc ccccgagcag cattgtcggc agcttgatgc cggtcaggat
     3121 tcccagtccc tgcttgatcc agaaatctct gctgccgagc cgtttcttca acagacgctc
     3181 cgccgaaagc ggcgttccgt cctgcgtcga ggtgttcata tcaccggcaa cgacgaccgt
     3241 gccggcagtg tctttgatcc gcgcgagaag ttcttcgagc tgtttgcggc gaccttcggg
     3301 cttggtcttg tcttccagat gcgtggcgac gacgaccagc cgcccgccgg ggatgcgctc
     3361 gtcggcgatc tcagccgtca acatcatgcg ccccccgcgc cgtacttccc gtcgaatttt
     3421 ttcctgaaac accagctcgc ccgccttgcg cttgccggct tcgagcggtc tgacgctttc
     3481 cagttcgtct ttgtaccaat cgtgcggctg ttgctcgaat ggttcgagcc ggacgttctc
     3541 taacgggaag cggctgagga tggcgttgcc gtgcagtccc cggtagcgcc ctcggttaac
     3601 gctgatctcc gcgatgagtt tggcggcgtc ttccggcggc agttcttcaa acttttccag
     3661 ccccagcgca atcgggtcaa cctcgacgaa ctcgacgccg taggcgtagt tcatcccaag
     3721 cgcagacgcc aaatcctcgg ccacgttgcg gtagtccgtg cgcctcatcc cgaagtccac
     3781 ttcgttgagc acgatcacgt ccgcctgctt cagaagctga acctgctgca agatcagtcc
     3841 ccgcttttca cttcctcggg ggtattcgga cgaatctatt agtcgggcga aggctgccgc
     3901 gtcggtgaac gcgagcctga tggcttcgta ctctagtccg cgctcgatgt tccacagggc
     3961 gacccgcagg agctgtccga gcttcgccgt gctcggcagg agcggcctca ccccgcgcgc
     4021 cgtcgcggcg ttgctgacga acggcgtcgt cagcagaaga cgtagcttgt tctgcaaagc
     4081 ctcaggcggt gtctcctgct cgtagagttg gacgagttcg tcgtaggtga gcaactccgg
     4141 cgcttgttgc cgcgcggcgg ccgcgacggg aatcaacaga cacgcgaacg aagcgagtag
     4201 caggagcttg aatttcaagg cggggcctct ccgcgcaggc cacggatctg cgagcttctg
     4261 tttcttttgc cgatttcctg tcacgcgcac tcgattgtag tctccgaagt agtgtgccgg
     4321 attgacgatt cgccggggcc tggtgttctc cagatgcggc agaccacctc cccggccggc
     4381 cgaattgcta cccgcagccc tctcgatcct gctggaaacg gatgcagcag attagccgaa
     4441 tccgaacggg gcagtctttt ctcatcttga cttttatttt ctaatcttga ctctttgtgt
     4501 gtgagctttc ccggtgcgac agggcgggcg cgaatatttt caggtcgttt gtggcaggcg
     4561 ggctcggtac gtcgatgaac gcttcccgga tgatcgccgc gggatgctcc tttgggagga
     4621 ccccgcggcg attcatactt gcgcttcttc gacttcttcg tctggcgtct tcatcttgcc
     4681 aagacggccg aagagcttcg caaacagagc gtaaatgcgc tctgactctt tcgccaataa
     4741 cggcgaagcg ctcgccagaa tcaacacata cagagcggcg aacggctgca agaccgccat
     4801 caagccgccg gcgagagcaa ggttgacgat gatgatggaa aactcgccgc gcgcgagcaa
     4861 agtgaacccg gtgttgagcg agccgacggt tgacagtttg gcgcgcctgc cgacaatcaa
     4921 tccggcgatg atgacgccga cgagcgaaag cagcgccgca cccgtagcca gccacgccgc
     4981 tcctcccaaa gtgaacgggt caatcgaaag accgaagccg aaaaagaaga tcgcgccgaa
     5041 gaaatcgcgg aacggcacga cgagtcgctc cattcgctcg ctgtgctcgg tctcgccgag
     5101 gatcaacccc agaagcagcg cgccgataga ttcggcgacg tgaatggtct cgccgagtcc
     5161 ggcgaggagg aacaggcagg cgaaaaccac gacgacgaag acctcgttcg aggagatgct
     5221 cagaaggcgg ttcaggagtg gcgtcgccca cctcccggcc accatcaggc cgatgatgaa
     5281 gccgagcgcg atgccgccag acgagagaac tccgccgagc gaggtcgcgc cgctcagcac
     5341 gatgccggag atgagtgaca gatagacggc gagaaaaacg tcctcgaaca tcgtgatgcc
     5401 gagtacgatt tgggtttcgg gattcgccat gcgccggtag tcgaagagga ctttggcgac
     5461 gatggcgctc gacgaaacgg tcgtcaagcc ggcgataatc agcgcctcgc gcagaggaaa
     5521 gcccgcgaga tagccgaagg tgaacccgac ggtgaaattg atgctgacgt agatcgtacc
     5581 gcccacgagg attgagcgcc ccgcttcgat caaacgcgag acgttggatt ccaggccgag
     5641 gtagaagagc aggaacagga cgccgatgcg ccccatgtat tcgatcagcg aaccggtctc
     5701 gatgaagcgg aagtcgaaga cgccgaactg cggggcgtga ggcccgaccg ccatgccgat
     5761 gatgatcagg aatgggatgt tggacaggcc gagtctgtgg gcgagcacga cagcgaaagc
     5821 aatcagcacc agcgccaggc cgatttctaa gatgatatta tccaccggct caacccgccc
     5881 ttccttcgac cagcaggcgc ttgacggcgt tgatcgtgct gcggtcgccc gcgaggatga
     5941 gcgttgcgcc ttcgttcaag acggtgtcgg cttcagggtt gaggcgcttc gtttggtctg
     6001 cttcaagtat cgagacgatg gacgcgcccg tcgcggtgcg aacctgaagg tcgcggatag
     6061 ttctgcccac gcacgccgct cctttttcaa tcgtgaacca ctccagcacg aggtctccta
     6121 agatgacctc cgccatcggg aggcttcgcg gcacgtatgt cagcccgccg ataatcccgg
     6181 cgatctgccg cgcctcgccg tcggagagcc tcaagaccga ggccgggcgc tccagattgc
     6241 ggcgcgtgaa gtgatacagc tcgcgcgtgc cgtcgtcgtg gatcacgacg accagccggt
     6301 caccgcttat agtctctacc tgaaacttgc gcccgacgcc ggggagaaac gcttcgctga
     6361 tggtggacat gaccttgaga cctttccgcg cctctccggg ataatctgag acccggcgcg
     6421 ttgactacat cattagctgt tcttcagtat cacaacacgt tttcataaat ttcacatggg
     6481 agacttggtt tgcggcgtcc cccgcgcgaa atcatctcat gcgtgcgccc tactactatc
     6541 gtaagagaga agtttacgcc cgaacttact tgccggcgac gaataaatcc gcgtcatccc
     6601 ctgcccggac ttccgccgct tcggccttct gtttgtcccg gcgcgaaccc gtagccgtcc
     6661 gcataagagc cacagtagtt atgaacacaa tggcgggtat caacattatc tctaaatcga
     6721 caatattctc taagccgtct gcgacgtgaa gccgctcgta ggccgcaagg gccagcagcg
     6781 ccagcccgtc gaggacgaag agcacgccga gcgcgaagtg gaagatcgtc agtggctttt
     6841 ccccgtagga tcgtgatcgc gtggagcgat ccttagagta gagcgcacgc actctcgaca
     6901 cgtggtcgaa gcacagccag ccgaagaaga gcgacaatgt cccgacagct atctctaaca
     6961 tgacccgcaa acttctcccg atttatcagt gtcacctttt tagcccaggt acgggcggca
     7021 ctctcgcccc gcagcgtgcg cgcttaacct cgctcatcaa cttctctttc ggcacgacct
     7081 gcccagcgcc cgcacttcgg acaccacctc gtcaacatct ccagcgtcgt ccttgccgtc
     7141 gtctcggcaa taatccatcc aaagatcggg ttcatctcac tcccctccct ctatttttat
     7201 gagcaatccc gagcgcacga cctcgaccga gcaggtcgcg ttcgtcacca gcgccgccga
     7261 aacgccgccc aagcccgatc tctcgtccgg gtgatccagc ccgtgtgagc cgacgaagat
     7321 ggagtcggct gcccactcgc gcgcctcgtc tatcagcacg cgccgcgggt caccttccct
     7381 gatgacgacc gatgcgtcca acccttcatc gagcaacacc acccgcgccc cttctgccat
     7441 caggtgcgcg ttcacgggcg ggccttcatt gcagcccgtg tacagttcct ccaacctcgg
     7501 cttgatgtcg gttatcctcg tggggccgct cccgtcgtcc accacgataa ggcggacctc
     7561 ggttccgtca ggccacgcgc gcatccccac ggcacgtacc gcccgcgccg cgccgcgcga
     7621 cccgtccacg ccgacgatga tgcgcggcgg agccacatcg tttttctcga ccccgcgccg
     7681 tccgacgcga accgagcagc gagcattggt ggcgagcgct ttcgacacgc tgccgaggaa
     7741 gaatcgcccc aaggcagagc gcccctgcga gccgaccacg accaggtccg gtcgccactc
     7801 ttcggccttt cgcagcaact cccgagaggg tgaaccctct aagacctcgg cgcgcacttc
     7861 ccagccgggg aagtatgatc ggacgcgcct gctcgcttcc gtcgcaaacc cccgcgcctc
     7921 tgcgagcagc cgcgcggcct gctcctttgc cacagcgatg gccgaggtca ccctgcgcgc
     7981 tgtcagcgcg gttccggcga cctcggccat cggtgtagag gtgctcacca ggccgtcgcc
     8041 caccgagacg atcagggcct cggcctcgcg cggcagaccg gcgcggcgca agtcatccag
     8101 cgcggcgtcg gcgtaagccg agccgtcgta tgcgatcaac actttcattc ggtttcccat
     8161 aactcaccat ccctccttgc gcgtcgcccg tcgcccttgc cgttaaaagg gcttctcgtt
     8221 ttctcaaccc tactccggcg gcgctcgctc gcctccagcc acgcgtacca tgcgaacagg
     8281 agtaccagca gaatcaccgc cacgatgatt gacttgaaat ccataccgcc tccgtcttca
     8341 tcaacaacct gatcccgcca gggccttcaa gctgcatcct gacgctttcg ttttgtcgcg
     8401 ccgcgcgcct gatcgaggga gggagtagtt gtcaacttgt tgtcatcggc tacggccgcc
     8461 ctttcattta gagttgactc ggttaactcc ttatccctac cttccaagtc cgagaacgcg
     8521 acgacgagca gcagcccgca catggcgagc gtcgaaagca cgtaaaggag cgcggtctgc
     8581 tcccagtaga gcagggcgga gacgaaggcc gccgcgctca agacccggat gaggctgccg
     8641 cgccgtccct ttcgccctcc cggtgtgtta attctccgac tcaattgacc acctcccccg
     8701 cttgcggcgc gcccgagtct ctctgacctt cgcgcccgcg cgccgccgta aggcgctcgt
     8761 caccccttcc cccctgattg cttaggggct tgcttcggcg ggatggtggc cgcgcacctc
     8821 tcgcacgcca cggcctcgaa caattccccc tccgaagtaa gctgattgtg cccgcattgc
     8881 gggcaacggc gtgtcagcat cctgcgccgt gtttcgccgt atgcccgcgt cgcaaccaaa
     8941 gcatgaatca tctgtccaac ctcctgctgc ctgctgaatt aaccatcttc agcttagtgg
     9001 atgcggggct ggcgaaaaac cgtgacatca cccgactcgt ctaggtgttt tcacgtgctc
     9061 cgcttccgct tagggttgca ccgacggaga gtgggggacg cccgccagcg tcgaattcat
     9121 gaatgaagta gccggtaaat atgaagggag ttgatgaggt gaagaagctg aagggcggag
     9181 acaaagccgc gccagagtgg gcggcaggtt agccggacgc accgcgcccg agcacgtggc
     9241 gcgcgatctc gacgcggttg ctccagcctt ttttagagag gatgttgctg atgtgccctt
     9301 cgacggtgcg gacgctcaac cccagctcgg cggcgatctc tttgttgctc aagccgtcgg
     9361 cgaccagacg tgcgacgcgg gcttcggcgt cggtgaaatc ttcgtcggga gcgtagtgcg
     9421 acggcgaact ctcttcgcct ttcgcggtgc tcacgagccg cgcgagttcg gcgccggtgc
     9481 gctcggcgcg gctgaggaga ttagagacga cggcgagcag ctcctccggg tcgaaaggct
     9541 ttgtgaggta cgcgtcaacg cccgtgcgga ggcccgcgat gcgatctttc cgctcgcctt
     9601 tcgcggtgag gaagatgatg gggatgagtt cggtgcgcgg gttcgagcgc agtgcgcgcg
     9661 cgagttgata gccgtccatg ccgggcatcc gtatgtcact gatgattaag tccggcacgc
     9721 ctctctggat cgagacgagc gcctcggcgc cgctccgagc ggtcgtcacc tcgtagcctt
     9781 cttcccgcag ggtgacggcg acggcgcgca acagtttcgc ctcgtcgtcc acgaccagca
     9841 gtcgtttagc catttgacaa cccctctcgc ttcacagcgc cactcgccag ggactcagca
     9901 ggcgctttga cagagcttgt cgcccaccca ccctcgtcaa cattagcttc atcatcagtt
     9961 tcatcccgcc acaccgggag ccgcaaagta aaagtgctgc cgcgtccgac ccggctgctc
    10021 gcctcaatcg agccgcccat tccttcgacg agcacgctcg ccaggtgaag gccgagaccg
    10081 atgccgggca cttcctgctc gtccaggtgg ccggcgccgt cacccgcccc ggcgcggccg
    10141 cgatagaagc ggtcgaagat gcgcggcaag tcttcggtgc gtatcccgac gccggtgtct
    10201 tcgacctcaa tcgcgacgcg cccgccgtct tcgtcacggc gggcgcgcaa gattatgcgc
    10261 ccgccgtcgg gcgtgtactt gatcgcgttc tcgacgacgg cgcagagcgc gcggcggagc
    10321 gcgctcttgt cggcgcggac gggcggcagg tcggagactg gtccgactac gagctcctga
    10381 ttgcgctcga cggctgcggc gcgctcgatc ttctcgcaag cccgcaacgc ctcgcccacg
    10441 tcaactcgcg cagccctaat ctcgacgccg cccactttaa cgcgcgacag gtcgagcagg
    10501 ttgtgcacga ggtcaatctg ccgttcgcac tccgaggcga tgtcttcgag gtactcgcgc
    10561 cgctcttctt cggtcggatt cttcctcaag agcacgcgcg tcaaagtctt aatcgtcgtc
    10621 agcggcgtgc gcagctcgtg cgagacggcg gcgaggaact cgtccttgac aacgttggcg
    10681 gcctcggctt cacgccgcgc ctgctgttcg agcgcgagcg ccctttcacg cgccgcgctg
    10741 agagaactga tgatgagcgc cgccagtgtg aagacgccga gttcaagcaa gtcctccccg
    10801 ttaactttga aagaatacgc cggcggaaaa aagaagaaag cgccgacgac cgccgagagc
    10861 gcggtggcga gcagccccgc gcccagcccg ccgcgccacg cgctgaacat gacggcggca
    10921 aggtaggccg acgagacatc catctcgacg tagcgcgtgg cgaacatcgt cagcacgaat
    10981 accgccccta ccgaccagac cgcgatcagg tagcgacgca gtttcttgcc cgtattttcg
    11041 cccaaggcgc tcacgaaact ttttccccac cggcgtcacc cgttgcggca gtggatgagc
    11101 cgccggcgtc ttcctcttcc ttctccgcct tacgtcgttt cactccgagc acccagccgg
    11161 gcgggtcgct cgccgggaaa gaatcttgca ggtctctgtc tatcttcgct tcgatcctgt
    11221 cttcgccccc cgcgccagtc ggcagccctt ccctttgctt cttctgctta gacttgctat
    11281 ccatctttcc acctccgttc ggcatgcggt tattagtagc gctttcctaa cgtataggac
    11341 agcgacgcag ccgcgatcca ccgtatggaa agcgccgttt acaacgcgcc tccccagata
    11401 actgggggcg acaaaggtca gccattgagc tgaaagattg atcggttttt gtcttaccag
    11461 cttaataagc ggtcggactc tgccttgact acctggtagc actcacaaac gaactcttcc
    11521 aatcctcccc tatcgaggat agtaattacc ccgcgtgagt agcgaatcag cccggcgttt
    11581 tgcaggatga tcgccgcctc gctgacccgg gagcggcgcg tgccgagcgt ctgcgcgagg
    11641 aactcttgcg tcagcctaag ctcaactcct ccaacacgat cctgacacaa caacagccag
    11701 cgcgctaatc tctcgccgag attatgattg cggttgcagg ccgccgactg ggagacctgc
    11761 ttgagcagcg ccagcgaata gcgcagcagc aggctttgca gtttgccgcc gcgcttgagt
    11821 tcttccttga gcttccctgt gcccatccgc atcgccccgt ccgggatctg cacgatggca
    11881 tgattttgag agacgtcgtc tcccatcacg acggagagac cgaccattcc ctcatttcct
    11941 actaagccga cttcgacact cgtcccccct tccatgtggg tgacgagcga gacgacggca
    12001 ccgtttaaga agtagacata tttgatgcgg ccttccatct cgtaaagagt ctctccgacg
    12061 ggcatagaga ccagctttaa atgtggaagc aggcggttat attctttagt cggcagggca
    12121 gcgagaatct tattctgggg tgtgggtggt ctggctgatt taggcatcgc atctccttgc
    12181 tttccgttcg cgcggggaac acaagctgta gcaggggaag atgcgtcgtg aaggcaagtc
    12241 ctgatgtcgc aagctgggtt ctgaaagaac agtaacggct tcaatattgc gtcactttat
    12301 cacaccggca ctgccatgtc gcacttccgc gcacatggct atgcacgaat tcgggagtat
    12361 aaccttgact ccctactatg cagggcatag actccccgtc attaagcttg tttcctgctg
    12421 aaactttaat ccttcaacgt aggacagcac ctacctagaa gcctacgttc cagccttaat
    12481 ctttctccag ccaacaccct taatggcgtg aacgttgtgt acgtcggctt tcttggacgc
    12541 agcgcaatcc caagaacgcg aaacctccaa gctctctcgc cggaaggaag ggaggaggac
    12601 atgattaagg taagaacggc aggcgggcgc ggaaaagctc tgaccaaggg tggtgacggc
    12661 cgtcgcacat tttcacaaga taggatttta gtctccgtca aggacggaac ctccaagcca
    12721 ttgttccctt ccgacgagcc ttctttgacg agcactgcct gcgcttgggg cggactcctt
    12781 gtggaacatc atcgcctgcc gcccgcagaa ttcccggaac gcgtcgcgac cagccatctc
    12841 gtcgccttgc atctgcgtcc ggcaaaattg gaatggtttc tcggcggaca cccgcagacc
    12901 aggcgcatga cgcgcggcag catcgacatt gtcccgcaag gcacgctctt gggaggttac
    12961 agtcgggatg agacggagtt tctgatgatg gcgctcgatc cgtcattcgt cgagcgcgtg
    13021 gcgagcgaag cgggtgtggc aatccctatt gaattggtca ggcatctcgg aatacgcgat
    13081 ccgcagatcg aacacatcgg cttggcgctc aaggcggaac tcgaaacggg ctgcctttcc
    13141 ggccggcttt acggcgaatc gctcgccgtc gcgctggcgg ctcacctgct cggcaagtac
    13201 gcggcgaatg ttcccaagac tcacagcgac aacgccgccc ttcccgctta caagctgcgg
    13261 cgggtgatcg agtatatcaa cgacaatttg actgaagatt taacgctcgc cgaaatcgct
    13321 cgtgtcgcgg ggatgaatcc gcattacttc tcccgcgcgt tcagacagtc aaccggcatc
    13381 ccgccgcaca ggtatgtcat caactgccgg gtggagaaag ccaagagatt gctggcagac
    13441 gatgaactgc ctctggtaga ggtcgggctg acggtcggct tccagaacca aagtcatttc
    13501 accacgctct tccacaagct gacgggcgtg acgccgaaag cctaccgcga cgaaacttag
    13561 tcgaagtccg tttgtgccgt ttcaactccc tcccccaaga aacttcccca accttaaacc
    13621 ctccctgagc aaaggctcgc ccgccctgcc atccattcag gctcacacga gaaagagtca
    13681 agattagaaa ataaaagtca agatgagaaa agactgccga tgtggactcg gctagagttg
    13741 caatctctcc tcccagtgga gcggctgcga gtcgccggcg attttcggcg ctggcagttt
    13801 gcggcgcttt taccaaagcg ccaacaagaa aaagacgaca gacattgagg agagctaaag
    13861 atggacggtg cgctcgtgaa agaaggcggc gtgtcatgcc tcgttttgcc ggtcggcgcg
    13921 cgcgatcacg cgcagggctc tatggaagcg gctgtcacgc tgctcgaata cggcgacttc
    13981 gagtgtgagc attgcgccga agccttcccc gtaataatga agatcaaacg gtggctgcac
    14041 ggtgcgttgt gctacgtgtt ccgccactcg cccgccgccg agaaacaccc gcacgcgctc
    14101 cgggcggcgg aagcggcgga agccgccgga gcgcaagggc gcttttggga gatgcatgat
    14161 ttgttatgcg agcgcagtcc tgccatcgat gacgttcacc tgaagcgcta cgcgcaggaa
    14221 ttaggtcttg acctggaacg cttcgagcgt gaaatgcggg agcgcgttca cctgggaaga
    14281 gtgcgtgaag atttcgagag cggcgtcgcc agcggcgtca gcgacacgcc ggcgtttttc
    14341 atcaacggcg tgcggtacga aggtgaggct gacttggaca gcctgctcgc ggctattgag
    14401 gaggctggga catcaagtca tgggtgactc cgagataaat tgaacgtgat tctccaaagg
    14461 gagaggttat cgctgaaagg agcgagagat gatcagcgac agttcatact aaagcgaagc
    14521 agttcgacct gagcggcctc aacggcatct acggtcgagt tcgagtatca gcgtgagggc
    14581 gaggatgagg aagttgagat cgaagtgaag tggaagcggc ggtaacgaca catgagaagc
    14641 atcccgcgaa aaaattcact cgtcaccgtg atacctaaac ctgcgcgtgc gtgggtaggc
    14701 gtcttactcg cctgtttagc tctcgccgct tgttcgcagg cgacgaagag ggccgaggat
    14761 gcgagcagca atcgcggttc tgccgcgtca actcaagcga cgcccgcatc agccaacacg
    14821 gctcaggcca ccccttcatc cgtcgcgccg agtccgacgc cggacgttgc gccgctgaag
    14881 atcccggaga gagtggcttc gggcgaagtc accgagtcag gcgggacgtg gaacagggcg
    14941 gtgcaaagca tcacctacgt gagttccgcc gacaacacgc gccagccgat gatgttctac
    15001 aagcccgaga cggatgaacc gagaccactg ctcgtcgccc tgcattcttg gagcgaagat
    15061 taccgacaag ccgagagcgt catctactcc gagtggtgcg tcgccaacga ttgggtcttc
    15121 attcacccga acttcagggg agcgaatacg agaccgcagg ctacggggtc ggagctggtc
    15181 atcggagatg ttctgagcgc gattgattat gcgaaagcga acgcccgcgt ggacgagagc
    15241 cgcatctacg cggtcggctg gtctggcggc ggctacctgg gactgctgct ggctggacgt
    15301 cagcctgaga tttgggcggg cgtgtcggcg tgggtgccga tctcggattt gaacgcgtgg
    15361 tacgaagaga gcaggcgctt ggggacgaag tatgtcagcc agattgcggc ttcgtgcggc
    15421 ggcagaccca caggcgagac gccggcagcg gcggagtgcc ggaagcgttc ggcgctcacc
    15481 taccttgagc gtgcccgcgg tgttccgatt gacatcaacc acggcatccg cgacgggcgc
    15541 aacaacgacg atccagtacc catcggccaa tctctgcgcg ccttcaacct gctggcagcg
    15601 cctcaagacc gcttcacgga agaggaggtc gcatacttca cgcgcgaggc ccaggtcccg
    15661 cccgctttgc gaagcgaaac gtcagaccct tcatacggag gtctgagaat acttttccgg
    15721 cggcaatcag gaaacgcgcg cgtcaccgtc ttcgacggcg ctcacgacaa gaacaccgaa
    15781 gccgctttca gatggctgaa ccggcacagg agagacagat agccgcgagg tgaacttaaa
    15841 tgtttgagtt attcaaaagc atcatcccgt atatcacgcc gatatttgtg atttccacga
    15901 tgctgaacgt cggtttgacg cagaaattat ccgacgttat cgggcatttg aaaaattatc
    15961 cttttgttct aaaaatgctc gtcgccaact tcatcgtcgc gccgctcgtg atgttgtcga
    16021 tgctctatgt cgcgccgttc gagccgacgc tcaaaggcgg actgctgatt ttctcgatgt
    16081 gcgcgggcgc gccgtttctc atcaagctga cgcagacggc agaacaaaac ctggcgctcg
    16141 gcgcggcggt gatgatgctt ttgatcgtcg ggacggtcgt ttatgtgccg attgtgctgc
    16201 cgttgatttt gtcggacttg accgtcgatg cctgggcggt cgccaaatcg ctgacgacgc
    16261 agatgcttct gccgattgtc gtcggaatgc tggctgtgca gtttctgcct gctttatcca
    16321 gaagagtgca gccgtgggtc ggtcgcttag gcaacatcac tttatacgct ttaatcgcgg
    16381 ccactttaat cggctatttg cccggcatgt tcggcatcgt cggtacgggc gcgattctcg
    16441 tcggcttggc gttcgtcctg gcggcgttcg gcttaggtta tctgacgggc gcggggaaag
    16501 atcatcttga ggatgtcggc ggtttaggca cggcgcagcg taacaccgcc gccgcaacga
    16561 ttatcgccgt gcagaatttc agcgattacc cggacgttct ggtgatcatc acgctcgcca
    16621 acacgctcgg catcgtgatg ctgatgttca tcgctaaacg gctgagccgc gacaacaagg
    16681 tggagatagt aacagtctga acattcatcg gacttcctcc gattctgcct gaagaaatcg
    16741 cgccggggga aggtatgacc ggagaagcag ccgaaaatca cagggaggaa gtgacggaga
    16801 aaaggagacg gacatgagcc aacgttcacc gctgaaaatc gtctgtttga taatcctggt
    16861 tacaagtgcg gtcgctctgt cgccgggggg ccggacgacg ggcaaggcac agggaaccgc
    16921 cgccgctaag gagcgcaagg gctgcgataa gggcgacggc ggtctcacgc tgcccgcagg
    16981 attttgcgcc tcggtgtttg ccgacaacct cgggcgcgcg cgtcatctca ccgccgcgcc
    17041 caacggggac gtttacgtga acacctggag cagtacgtac accaatctga agaacgcgcc
    17101 gggtggttac atcgtggccc tgcgcgacac cgaccgcgac ggcagagccg atatggtcga
    17161 gcgtttcggc cccgcgcatc aggacgggaa ggcgggcggc ggaaccggca tcgccgtgca
    17221 tcgcggcgcg ctgtacgtcg aggacatcgg aaaaatcgtg cgctaccggc tcggtcgcgg
    17281 ccggctggtc ccgcagggga atcctgacgt catcttgagc ggtttgtgga ccgagcgcgg
    17341 ccacatcatg cacccgtttg cgatcacgtc cggcggagtc ctgtacgtga actcaggttc
    17401 gatcacgaac tcgtgtcagg agaaggaccg cacgctggag tcgccgggaa tttcgccgtg
    17461 cccggagctt cagctacacg cgggcatctg gcgttacgac gccggcaaga ccggccagac
    17521 tttctccccc gccgaacgct tcgccaccgg cacccgcaac gtcgtcgcgc tggcggtgaa
    17581 cggggcggac ggggcgctgt atgccacttt gcacgggcgc gacaatttgg ccgcccactg
    17641 gccgaagctc tacaccgcgg agcagaacaa cgaactgccc gcggaagtct tcgcgcgcat
    17701 caaccgcggc gacgacttcg gctggcccta ctgctacttc gaccctcaac agcgcaagca
    17761 cgtgctcgcg ccggaatacg gcggggacgg cgggaaggcg cagggcgtct gcgcgcagaa
    17821 gaagcaaccc gacctgacct tccccgcgca ctgggctccc aacgggatga cgttctatcg
    17881 tggctcgacg tttccggcga agtaccgcgg cggcgccttc gtggccttcc acggttccca
    17941 tgacagaacg ccgacgcagg cgggctatcg ggtggcgttc gtgccgttcg aggcggccag
    18001 gccggcgggc cgctacgaag agttcgcgac gggttttgcc ggcgcggggc cgcttgcggc
    18061 tcctaccgaa gcgccccacc ggcccatggg cctggcggtt ggccctgatg gagcgctgta
    18121 cgtctccgat gacgtcaaag ggcggatttg gagaatcaca tacgtcggcg gcgtctgacc
//
LOCUS       DASVES010000286         6394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_273058, whole genome shotgun sequence.
ACCESSION   DASVES010000286 DASVES010000000
VERSION     DASVES010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273058"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF240_11535"
     CDS             complement(<1..242)
                     /locus_tag="VF240_11535"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX7175881.1"
                     /translation="MNELSELRRAAREIFDHALAESDAGRAVRRAVRLDGARLNIFDD
                     EFDLDRPARVYSVAAGKAARPMAAALDEALGARLIRG"
     gene            complement(392..748)
                     /locus_tag="VF240_11540"
     CDS             complement(392..748)
                     /locus_tag="VF240_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7175882.1"
                     /translation="MRRRDYSTAGSRFALCCFLTLATAPSCVRTPRRQPAVGRGDARQ
                     TALAAATQPGPRVNINKASRAELERLPGVGPSSAARIVEHRERYGPFRRAEHLLLVRG
                     FSDSRFRELRPLIAVD"
     gene            complement(1069..3825)
                     /locus_tag="VF240_11545"
     CDS             complement(1069..3825)
                     /locus_tag="VF240_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX7175883.1"
                     /translation="MATMQTDVRVKTTGATVRPPSQAPRNSRRNEIIAIALLAVSALL
                     VLCMLSYSSFDPSWNASGQGEARNLVGRVGANVSAALFQTFGFAALLVPALMLAAAWR
                     RFRTRRIHAPLARVVGLLLLVFAVAALLDLYVPRESFGSGFESGGVVGVLVSDGLSGM
                     VNTVGATILLAALAATGVLLATNFSFARAYDRLEAAANDPSSSVHKARERFNAWRAER
                     RTRAEQRVELRRAARAERESVLLEAQKTLAADLAAQPTIAPADASPKRRSQSPSAAGA
                     IQRAAAAGAVAGEKPTREDEIRAKLEQAEAELRAITLHPTSAAPEAPLKRVPTTRAAG
                     KSTNATAAEDAPPALRRNVVPQPLPASPYVAGQPVVDEADIEEMLREAEVVRTEGDLL
                     DAAVAPPVKRRAAAGESPQEPSTRVEKSLAALEYKLPPLEFLNAPPPRTEQADEELLQ
                     IASRVAEKCREFNVTGQIKHICPGPVVTTYEFKPDPGVKYSRVTGLVDDLCIALEAES
                     IRIDRLPGKPHVGIEVPNPRRETIQLREVLESRQFRDSESKLTLALGKTIDGMNYVAD
                     LTRMPHLLIAGATGTGKSVCLNSLVVSVLYKARPDEVKFIMIDPKRLELGLYADIPHL
                     ATPIITEPKKAANALKWAVGQMEQRYKDLARWGVRNIDSYNTEVERRNNVLDYGDNGQ
                     PHESLPYIVIIIDELADLMMTCGSDVEEAITRLAQMARAVGIHLVLATQRPSVDVITG
                     LIKANFPSRIAFRVSSKVDSRTIIDSNGAEQLLGRGDMLFLPPGSARLVRVHGAYLDE
                     TEVAQIVAHIKAQSAPVYDQTITQSEDETGGADGNGGERDELFDQALRICCEMKRAST
                     SVLQRRLRIGYGRAAAILDGLERDGFIGQADGARPRPVLGRAFETIAHWDDLQEGEAA
                     EEDY"
     gene            complement(3927..5040)
                     /gene="prfB"
                     /locus_tag="VF240_11550"
     CDS             complement(join(3927..4967,4969..5040))
                     /gene="prfB"
                     /locus_tag="VF240_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX7175884.1"
                     /translation="MLETASVQDLRTSYEDLRTRVEQLGGFFDVPAKREELKRIEAQA
                     SAPDFWSDQEEAQKLMGRRSRLEKSAARHEKFESEISDASVLFEFAEEEDADSLGELR
                     ALVGRLEREIKEAETEMLLSGESDARNAICTIHPGAGGTESQDWAEMLLKMYLKWAER
                     RGFETEIIEYQPGDEAGLKSVTFTVKGEYAYGLLAAEAGVHRLVRISPFDQAARRHTS
                     FASLFVYPEVEEDIEVEINEKDLRVDTYRSTGAGGQHINTTDSAVRITHLPTNIVVAC
                     QNQRSQHQNRAVAMNVLRARLYELELEKRRAATAELEASKADISFGSQIRNYVLAPYR
                     LVKDARTKLERGDVDSVLAGDIDDFIQAYLLSRRAA"
     gene            complement(5079..>6394)
                     /gene="lnt"
                     /locus_tag="VF240_11555"
     CDS             complement(5079..>6394)
                     /gene="lnt"
                     /locus_tag="VF240_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7175885.1"
                     /translation="VLLIPAPVIAGLFPGAWAYSVARISARRGVGWALVVAPFAWVAL
                     EWARLGAIGQLWNALAYSQAFTPALIQSARWGGVYLVGFLIVSVNSAVAYLILRRNGR
                     ALSIAVGVALLVAAVVFLASYTNRVQQATTPSVAVVAVQPNVAADFPRSAGETALLLN
                     RHLTLATDALRELDGAQVATAGAGDDVRRGLPRVVVWPESPMNFRYARDENFRDFVAE
                     FARANRASVIFNAVEPAPMGGVYNSAVMVNEEGRLVAQYDKIRLLPFGEYVPLPRWLP
                     PAWLLDGLVGDFAMGNDYPLMPVGDARAGVFICFESAFPSVAGRFARDGADVLVNITN
                     DGYLGQTPVVRQHLANVVFRAVETGRPVLRVTNTGVSAHVTPRGEVLDATGTYRPDVR
                     TWTVGRAVSGKTFYARQGDLFVALCAAAAAAGLLSTFNFRRSRAT"
BASE COUNT         1088 a         2033 c         2189 g         1084 t
ORIGIN      
        1 ccgcggatga gacgagcgcc gagcgcctcg tcgagcgcgg cggccatcgg gcgcgcggcc
       61 ttacccgcag cgacggaata gacgcgcgct ggccggtcga ggtcgaattc atcgtcgaag
      121 atgttcagac gtgcgccgtc gagccggacg gcgcggcgca cggcgcggcc cgcgtccgac
      181 tcggcgagcg cgtggtcgaa gatttcgcgc gcggcgcggc gcagttcgga gagttcgttc
      241 aaggctcgga gggacgctta taactgacag taccgcgagc ggtagcgagc gggtacgact
      301 atgctgaaga ctcgcctcgc gcacgcgtga cccgctcgct accgctcgcg gtactgtcag
      361 cgcgtccgtc acacattgat tcgcaagcta tctaatcgac cgcgatcagg gggcgcagct
      421 cgcggaagcg tgagtcgctg aagccgcgca cgagcagcag gtgctcggcc ctgcggaaag
      481 ggccgtagcg ctcgcggtgt tcgacgatgc gcgcggccga cgacggcccg acgcccggca
      541 gccgctccaa ctctgcgcgc gaagccttgt taatgttgac gcgcggcccc ggctgagtcg
      601 cggcggcgag cgcggtctgt cgtgcgtcgc cgcgacccac ggccggctgg cggcgtggcg
      661 tgcggacaca ggagggggcg gtggcgagtg ttaaaaagca gcagagcgcg aagcggctgc
      721 cagcagttga ataatcacgt cgcctcatgg ggcgtaagtc tcagcttgga aagaatttgg
      781 gacctcccgc cgggcaggtg ccacgttcgt gtgcaaaatg ctataagcct aaacttttca
      841 gaaacttgta accccgaaac ccgatttatt cacagacttt tccacagaag ctgtggaaaa
      901 gtcacagcgc tgtttcgccc gaaatttcgt gccggctgca aaatatttaa cacggacagg
      961 aaggggtcga gggatgaggg atgaaaaaga ttgattcatt gaatcaatga atcgatgagt
     1021 caatgaatca atctttctca tccctcatcc ttcatccctc atccgttttc agtagtcttc
     1081 ttccgccgcc tcgccctctt gcaggtcatc ccagtgggcg atggtctcga aggcacggcc
     1141 taagacgggg cgggggcgtg cgccgtcggc ctgcccgatg aagccgtcgc gctccaaccc
     1201 gtcgaggatg gcggcggcgc gaccgtaacc gatgcggagg cgacgctgga gcacggaggt
     1261 cgaggcgcgc ttcatctcgc agcagatgcg cagcgcctga tcgaatagct cgtcgcgctc
     1321 gccgccgttg ccgtcggccc cgcccgtttc atcctcagac tgcgtgatgg tctggtcgta
     1381 gaccggcgcg ctctgcgctt tgatgtgagc gacgatctgc gccacctcgg tctcgtcgag
     1441 gtaagcgccg tggacgcgca cgaggcgcgc ggagccgggc ggcaggaaga gcatgtcgcc
     1501 gcgcccgagg agttgctcgg cgccgttcga gtcgatgatc gtgcgcgagt caaccttcga
     1561 ggagacgcgg aaggcgatgc gcgaggggaa gttggccttg atcagtccgg tgatgacatc
     1621 gacgctgggg cgctgcgtgg cgaggacgag atggatgccg acggcgcgtg ccatctgcgc
     1681 gagtcgcgtg atggcctcct ccacatcgga gccgcaggtc atcatcaggt cggccagctc
     1741 gtcgatgatg atgacgatgt aggggagcga ctcgtgcggc tggccgttgt caccgtaatc
     1801 gaggacgttg ttgcggcgct cgacctctgt gttgtagctg tcgatgttgc gcacgcccca
     1861 gcgcgcgagg tctttgtaac gctgctccat ctggccgacc gcccacttca gggcgttggc
     1921 ggccttcttc ggctcggtga tgatcggcgt ggcgaggtgc ggaatgtcgg cgtaaaggcc
     1981 aagctccaga cgcttcgggt cgatcatgat gaacttcacc tcgtcgggcc gcgccttgta
     2041 gagcacggag acgacgagcg agttgagaca cacagacttg ccggtgccgg tggcgcccgc
     2101 aatcaaaagg tgcggcatgc gcgtgaggtc ggcgacgtag ttcatcccat cgatggtctt
     2161 gcccagcgcg agcgtgagct tcgattcgga gtcgcggaac tggcgcgact ccagcacctc
     2221 gcgcagttgg atggtctcgc ggcgcgggtt gggcacctcg atgccgacgt gcggcttgcc
     2281 gggcaggcgg tcgatgcgaa tcgactcggc ttcgagcgcg atgcagaggt cgtcaacgag
     2341 acccgtgacg cgcgaatact tgacgccggg gtcgggcttg aactcgtagg tggtgacgac
     2401 ggggccgggg cagatgtgct tgatctgtcc cgtgacgttg aactcgcgac acttctcggc
     2461 gacgcgcgag gcgatctgca aaagctcttc gtcggcctgc tcggtgcgcg gcggcggcgc
     2521 gttcaaaaac tccagcggcg gcaacttgta ttcgagcgcg gcgagactct tctccacgcg
     2581 ggtggatggc tcctgcgggc tctctccggc ggcggcgcga cgcttcacgg gcggggccac
     2641 ggcggcgtcc aacaggtcgc cctccgtgcg gacgacctcc gcctcgcgca gcatctcttc
     2701 gatgtcggcc tcgtcaacga cgggctggcc cgccacgtaa ggcgaggctg gcaacggctg
     2761 tgggacaaca ttacgtcgca gcgcaggcgg cgcgtcctcc gcggccgtgg cgttggtgga
     2821 tttcccggcg gcgcgggtag tgggtacacg cttgagcggc gcttcgggcg ctgctgaagt
     2881 cggatgcagt gtgatggccc gcagttccgc ctcggcctgc tccagcttgg cgcgaatctc
     2941 gtcctcacgc gtgggcttct caccggcgac tgcgcccgcc gcggcggcgc gctgaatcgc
     3001 gccggcggcc gaaggagact gcgagcgccg cttcggggac gcgtccgccg gggcgatggt
     3061 gggctgcgcg gcgaggtcgg ccgccaacgt cttctgcgct tccaaaagca cgctctccct
     3121 ctcggcgcgg gccgcgcgcc gcaactcgac gcgctgctcg gcgcgcgtcc gccgctccgc
     3181 gcgccacgcg ttgaagcgtt cgcgcgcctt gtggacggag cttgaggggt cgttggcggc
     3241 ggcctccaga cggtcgtagg cgcgcgcgaa ggagaagttg gtggcgagca gtacacccgt
     3301 ggcggcgagc gcggcgagta gaatcgtcgc gccgacggtg ttgaccatcc cgcttagccc
     3361 atcggagacg agcacgccga ccacgccgcc cgactcgaag ccgctgccga agctctcgcg
     3421 cggcacgtag aggtcgagca gcgcggcgac ggcgaagacg agcaggagga ggccgacgac
     3481 gcgcgcgagc ggcgcgtgaa tgcggcgcgt gcggaagcga cgccaggcgg cggccagcat
     3541 cagcgcgggg acgagcagcg cggcgaagcc gaaggtctgg aagagcgccg ccgagacgtt
     3601 ggcgcccacg cggccgacga ggttgcgcgc ctccccctgc ccgctcgcgt tccacgacgg
     3661 gtcgaacgag ctataggaga gcatgcacaa cacgagcaga gcgctcacgg cgaggagcgc
     3721 aatcgcgatg atctcgttgc ggcgcgagtt gcgcggcgcc tgcgacgggg ggcgcacggt
     3781 cgcgccagtc gttttcaccc gcacgtcggt ctgcattgtc gccatagctt ctccaaaaac
     3841 tcagaggtca gaggttagag gttagaggtt agttaaaagc aggtctttca ctgacctcta
     3901 acctctgtcc catgacctct atttcgtcac gccgcgcggc gcgacagaag gtatgcctgg
     3961 atgaagtcgt cgatgtcgcc ggcgagcacc gaatcgacat cgccgcgctc cagcttggtg
     4021 cgcgcgtcct tcaccagccg gtagggcgcg agcacgtagt tgcggatctg tgagccgaag
     4081 gagatgtcgg ctttgctggc ttcgagttcg gccgtcgcgg cgcgacgctt ttcgagttcc
     4141 agctcgtaga gacgcgcgcg caggacgttc atggcgacgg cgcgattctg atgttgtgaa
     4201 cgctggttct ggcaggcgac gacgatgttc gtcggcagat gcgtgatgcg aacggccgag
     4261 tcggtggtgt tgatgtgctg gccgccggcg ccggtcgagc ggtaggtgtc cacgcgcaag
     4321 tccttctcgt taatctcgac ctcgatgtcc tcctcgactt cgggatagac gaagagggaa
     4381 gcgaaactcg tgtggcggcg cgccgcctga tcgaagggcg agatgcgcac tagccggtgg
     4441 acgccggcct cggccgcgag cagcccgtag gcgtattcgc ccttgacggt gaaggtcacg
     4501 ctcttgagtc cggcctcgtc gcccggctgg tactcgatta tctccgtctc gaagccgcgc
     4561 cgctcggccc acttcagata catcttcagg agcatctcgg cccagtcctg cgactcggtg
     4621 ccgcccgcgc cggggtggat ggtgcagatg gcgttgcggg cgtccgactc gcccgacaga
     4681 agcatctcgg tctccgcctc tttgatctcg cgctccaacc gcccgacgag ggcgcgcaac
     4741 tctccgagac tgtcggcgtc ctcctcctcg gcgaactcga agaggacgga ggcgtcggaa
     4801 atctccgact cgaacttctc gtgacgggcg gccgacttct ccagacgact ccgccggccc
     4861 atcagtttct gcgcctcctc ctgatccgac cagaagtcgg gcgccgacgc ctgagcctcg
     4921 atgcgcttca gctcctcgcg cttcgccggg acatcaaaga aacctcccga gctgttcgac
     4981 tcgcgtccgc aggtcttcat acgacgtgcg taaatcctgt actgatgctg tctctaacat
     5041 tttttttctc tctcaacaga aaatagtttg ctcgaacctt atgttgcgcg cgagcgcctg
     5101 aagttgaacg tggaaagcag gccggcggcg gccgctgccg cacacagggc gacgaagagg
     5161 tcgccctgtc tcgcgtaaaa agtcttgccg ctcacggcgc gcccgaccgt ccacgttcgc
     5221 acgtcaggcc ggtacgtccc cgtcgcgtcc agaacttcgc cgcgtggtgt gacgtgtgcg
     5281 ctcacccccg tgttcgtcac acgcaggacg ggcctgccgg tctcgacggc gcggaagacg
     5341 acgttggcga ggtgctgtcg gacgacggga gtctggccca gatatccgtc gttcgtgatg
     5401 ttcaccagca cgtcggcccc gtcgcgcgcg aagcgccccg ccacggacgg gaacgccgac
     5461 tcgaagcaga tgaagacgcc ggcgcgcgcg tcgcccacgg gcatcagcgg gtaatcgttc
     5521 cccatggcga agtcgccgac gaggccatcc aacagccatg ccggcgggag ccagcgcggc
     5581 aacggcacgt actcgccgaa cggcagcagg cgaatcttat catactgcgc gacgagccgg
     5641 ccctcctcat tcaccatcac cgccgagtta tagacgccgc ccatcggcgc cggctccacc
     5701 gcgttgaaga tgacggaggc gcggttggcg cgcgcgaact ccgccacgaa gtcgcggaag
     5761 ttctcgtcgc gcgcgtaacg gaagttcatc ggcgattcgg gccatacgac gacgcgcggt
     5821 agtccgcgcc gcacgtcgtc acccgcgccc gccgtcgcca cctgtgcgcc gtcaagttcg
     5881 cgcaacgcat ccgtcgcaag cgtgagatga cggttcagga gcagcgccgt ctccccggcc
     5941 gagcgcggga aatcggccgc cacgttcggc tgcacggcga cgacggccac gctcggcgtg
     6001 gtcgcttgct gaacgcggtt cgtgtatgaa gcgagaaata cgacggcggc gacgagtaag
     6061 gcaacgccaa ccgcgatgga gagtgcgcgt ccatttctgc gaagaatcag atacgcgacg
     6121 gccgagttaa ccgatacgat cagaaagccg acgaggtaga cgccgcccca gcgcgcgctt
     6181 tgaatcagcg cgggcgtgaa cgcctgcgag taagcgagcg cgttccagag ctgtccgatg
     6241 gcaccgagcc gcgcccattc gagcgccacc cacgcgaacg gggcgacgac gagcgcccac
     6301 ccgacgccgc gcctcgcaga gatgcgggcc acactgtaag cccacgcgcc ggggaagaga
     6361 ccggcgatga cgggggcggg gatgagcagg acga
//
LOCUS       DASVES010000287         5790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1691, whole genome shotgun sequence.
ACCESSION   DASVES010000287 DASVES010000000
VERSION     DASVES010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..1164)
                     /locus_tag="VF240_11560"
     CDS             complement(364..1164)
                     /locus_tag="VF240_11560"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEX7175886.1"
                     /translation="MSLRRKLRPLVAAVIAALALSCSERAEKSVNAPAAPPGAPELIG
                     RADALYLLREDTGRVREAVALLRRARTLDEGDYGAHWKLARSLHFLGETLADSGERDA
                     SYSEGIAAGQQAVRVNPAGVEGHYWLGANLAGRTEVQGVIGDLAAVGDVRREMEAVVA
                     ADEGYERGGAYLHLARVEMGLPRVLGGDRKRPVELLEKGLRFGGDNAGLRLQLARAFL
                     AARRREDARRELEALLAMEPNPDYVPEHRRAVAEAERLLKERFEKKAR"
     gene            1429..2091
                     /locus_tag="VF240_11565"
     CDS             1429..2091
                     /locus_tag="VF240_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7175887.1"
                     /translation="MAAAAFYDLEGTLVSTNLVHTLSFYARNQQGLLRSFRKSAATVM
                     SLPLFAVTDQYSRKVFNDLFFKRYRGETEDRLRFFSAELFENVLKPAIFPGTFDLIEK
                     SRRLGLRQVVVTGALTFSVDPLMRHLGIDDYVANRLEFVNGVATGRLLPPVLASATKA
                     KWIREFAEREGLNLSESYAYSDSMSDLPMLSIVGHPAAVNPDMRLRQTALHHDWPVLN
                     LK"
     gene            2167..3765
                     /locus_tag="VF240_11570"
     CDS             2167..3765
                     /locus_tag="VF240_11570"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX7175888.1"
                     /translation="MSLQLRRKVHESVVYIDRDSAPRIIPSGEDFILEDLPIGTRVIY
                     PNPPVKGLPNREAAVRYALNHPHDCEPLYAQLQPGMRVTVAVDDISLPLPPMATPDVR
                     QTILEVVLELLAANGVDDVHIIIANSLHRKMTEWEMKRMVGPKIFSEFYPDRYYNHDA
                     EWDEGLVKLGETRHHEPLRVNRRAVESDLLIYVNINLVPMDGGHKSVAVGLCDYESLR
                     AHHEPKTIRASDSYMDPARSELNNKVVRLGKLVDEHMNVFHIETALNNRMYTSEMDFL
                     LKNEDDFSAFDRLKFEAMRRGMSAMPRGARRKLLHSFPAQYQMIACHAGRTEPTHARI
                     LEKNFEQYAVPVTGQCDILITGIPDISPYNVYSILNPLLVQVMALGYHFNFYRNKPLL
                     KKGGVMIINHPCYDQFDHKFHPSYIEFFNRLLPETRDSDTLRDKYEREFAENPSYIEM
                     YRRGNAYHGAHPFFMWYWGENGRQHTGRIIAAGAENAHVPAMLGWERADNLTEAIEMA
                     RSYMGRSAEITMLHQPIIGICDMS"
     gene            3789..4367
                     /locus_tag="VF240_11575"
     CDS             3789..4367
                     /locus_tag="VF240_11575"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7175889.1"
                     /translation="MSTTTKLITADELLVMPHPDARGNKRVLELIRGELKIMSPTGLT
                     HGIICTELVVELTRFVKANNLGIVVGPETGCVVERNPDSVLGVDVAYVSHERMKGVEN
                     LEKFLPFAPDLAVEVLSPTNTAQEMDEKVALYFAGGAGAVWVFNPRRRTVAAYTSPFD
                     VRILGEQDVLDGGGVLEGFSLELSKLFSAINS"
     gene            4385..>5790
                     /locus_tag="VF240_11580"
     CDS             4385..>5790
                     /locus_tag="VF240_11580"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7175890.1"
                     /translation="MKLSPTEIYNGRNVFIIGATGFLGKVTLSMLLHRFPGVGRVYVT
                     VRARSQEESETRFWNNVITAPPFDPLRDLYGAAFEGFVRDKVSIVNGDIAQDNLGMTE
                     EEAQAVASDISVIINSAGNVTFNPPLESALRTNVVGTKNVIAFAKRMKRPALLHVSTC
                     FVAGNRSGPVWESDPVVGYFPRREEMPGVEFSVEQEIKDSERLAERVRDEVRDAVTAA
                     RFRELARKRLVEEGRDADDLDAMGLAVARERKMWTRERLTELGTERAHFWGWPNIYTY
                     TKSLGEQLVAAEEGIVKAIVRPSIVESAAEFPFPGWNEGFTTTAPLIFIALKGQTLIP
                     VNEKLILDITPVDSVAALMLAVAAEACVREPRLVYQAATGDSNPNTMERIVGLLGLYK
                     RQHFRDKDSGLRIVNEVAGRMETKPVTPSRFEKTSTPMVNAAAKKVSSLLDGVRPRWG
                     GGRLTEIIDRAKETVDRV"
BASE COUNT         1096 a         1966 c         1733 g          995 t
ORIGIN      
        1 ggggcggggc gcatgctcga cgcgctccac gcctcgggcc gcgacgccga gcacgtgccg
       61 ttgcattatt tgcgcgacga gttgaagttc tagaacgcgc gtgcccagcc ccgtcagggg
      121 cggaagagtg tagcccacgg cgtgagccgt gggacattcg tgagggaaaa gagaaaagcc
      181 ccggaggggc gaaagaatct caagcttgag cacattgctc gttgaaggct ctttcgcccc
      241 tccggggctt caaattcttt cgggcgtatg tcccccggtc ttacgaccgg ggctacatta
      301 tttcgcccct ccggggctcg gcacgcgcgt atccgtgttc tgtgtttcgt cagactttac
      361 aagtcaacgc gccttcttct caaaacgctc tttcaaaagc cgctccgcct cggccacggc
      421 ccggcggtgt tcggggacgt aatcgggatt cggctccatt gcgagaagcg cctcaagctc
      481 gcgccgcgcg tcttcgcggc ggcgggcggc gaggaaggcg cgggccagtt gcagccgcag
      541 acccgcgttg tcgcccccaa acctcaaccc cttctccagc agctccaccg gacgcttccg
      601 gtcgccgccg agcacgcgcg gcaaccccat ctccacgcgc gcgaggtgca ggtacgcgcc
      661 gccgcgttcg tacccttcgt ccgccgcgac gaccgcctcc atctcccgcc gcacgtcgcc
      721 cacggcggcg aggtcgccga tgacgccttg cacctccgtg cgcccggcca gattggcgcc
      781 cagccagtag tgcccctcga cgcccgcggg gttgacgcgc acggcctgtt gccccgccgc
      841 gatgccctcg ctgtaagacg cgtcgcgctc gcccgagtcc gccagcgtct cgccgaggaa
      901 gtgtaaagac ctcgcgagct tccagtgcgc gccgtagtcg ccctcgtcga gtgtccgcgc
      961 gcgccgcaac agcgcgacag cttcgcgcac gcggcccgtg tcttcgcgca gtaagtagag
     1021 cgcgtcagcg cgcccgatta gctcgggggc gccgggcggg gcggccggcg cgttaaccga
     1081 cttctcggcc cgctcggagc aggagagggc gagcgcggcg atgacggcgg cgacgagggg
     1141 tctcagtttt cttcgaaggc tcatggggga agtcggacgc gccgcgcggc aatccttctt
     1201 gtccacgcgg acgcgcggca gtatatcatc agcctttgtg tcgaagggcg acggtcgcgt
     1261 ttaagtcgct cacgctaaaa ggtttcgcgc gggcgtcggc ccggcaaaat acatttttgc
     1321 cgccgaataa ggtatagtgc agccctacgc gaacaattga accgggccga taggggttcg
     1381 ataaacaaaa ccattcgaca cgaagggacg ctgggggagg acgaccattt ggcggcagcc
     1441 gctttctacg acctcgaagg cacgctcgtg agcacgaacc tcgtgcacac gctgagcttc
     1501 tacgcgcgca accagcaggg cctcctgcgc agctttcgga agagcgcggc caccgtgatg
     1561 agcttgccgc tcttcgccgt caccgaccag tacagccgca aggtcttcaa cgatctcttc
     1621 ttcaaacgct accgcggcga gaccgaagac cgcctccgct tcttctccgc cgagctgttc
     1681 gaaaacgtgc tcaagccggc catcttcccc ggcaccttcg acctcatcga gaagtcgcgc
     1741 cgcttgggcc tgcggcaggt ggtcgtcacg ggcgcgctca ccttctccgt cgatccgctg
     1801 atgcgccacc tcggcattga cgactacgtc gccaaccgcc tggagttcgt caacggcgtg
     1861 gcgacgggtc ggctgctgcc gccggtgctg gcgtcggcca caaaggcgaa gtggatccgc
     1921 gagttcgccg agcgcgaggg gctgaacctc tcggagtctt acgcctactc cgacagcatg
     1981 agcgacctgc ccatgctctc catcgtcggc caccccgcgg ccgtcaaccc cgacatgcgt
     2041 ctgcgccaga cggcgctgca tcacgactgg ccggtgctga acttgaagtg ataagtgata
     2101 agtgatgagt gataagatgc tttttctgtc actcatcact tatcactcat cacttatcac
     2161 ttgcttatga gcctgcaact ccgaagaaaa gtccacgagt cggtcgtcta catcgaccgc
     2221 gacagcgcgc cgcgcatcat cccttcgggc gaggacttca tcctcgaaga cctgcccatc
     2281 ggcacgcgcg tcatctaccc gaacccgccc gtcaagggtt tgccgaaccg cgaggccgcc
     2341 gttcgctacg cgctcaatca cccacacgac tgcgagccgc tttacgcgca gctccagccg
     2401 gggatgcgcg tcaccgtcgc cgtggacgac ataagcttgc cgctcccgcc gatggccacg
     2461 cccgacgtgc ggcagaccat cctcgaagtc gtcctcgaac tgctcgccgc caacggcgtg
     2521 gacgacgttc acatcatcat cgccaactcg ctccaccgga agatgacgga atgggagatg
     2581 aagcggatgg tggggccgaa gattttctcg gagttctatc ccgaccgcta ctacaaccac
     2641 gacgccgagt gggacgaagg tcttgtgaag ctgggcgaga cgcgccacca cgaacccctg
     2701 cgcgtcaacc gccgcgccgt cgagtcagac cttctcatct acgtcaacat caatctcgtc
     2761 ccgatggacg gcgggcacaa gtcggtcgcc gtgggcctgt gcgactacga gtctctgcgc
     2821 gcgcaccacg agccgaagac catccgcgcc tccgacagct acatggatcc cgcgcgctcg
     2881 gagctaaaca acaaggtcgt ccgcctcggc aagctcgtcg atgagcacat gaacgtcttc
     2941 cacatcgaga cggccctcaa caaccggatg tacacctccg agatggactt cctcttaaag
     3001 aacgaggacg acttctcggc ctttgaccgc ctcaagttcg aggccatgcg gcgcggcatg
     3061 agcgccatgc cgcgcggcgc gcggcgcaaa ctcctccact cgttccccgc gcagtaccag
     3121 atgatcgcct gccacgccgg ccgcaccgag ccgacccacg cgcggattct ggagaagaac
     3181 ttcgagcagt acgccgtccc cgtcacgggg cagtgcgaca ttctcatcac gggcatcccc
     3241 gacatctcgc cctacaacgt ctactcgatc ttaaacccgc tgctcgtgca ggtgatggcg
     3301 ctcggctacc acttcaactt ctaccgcaac aagccgctcc tgaaaaaagg cggggtgatg
     3361 attatcaatc acccctgcta tgaccagttc gaccacaagt ttcacccgag ctacatcgag
     3421 ttcttcaacc gtctcctgcc cgagacgcgc gactcggaca ccctgcgcga caagtacgag
     3481 cgcgagttcg ccgaaaaccc ctcctacatc gagatgtacc ggcgcggcaa cgcctaccac
     3541 ggcgcgcacc ccttcttcat gtggtactgg ggcgagaatg gccgccagca caccggccgc
     3601 atcatcgccg ccggcgccga gaacgcccac gtccccgcca tgctcggctg ggagcgcgcc
     3661 gacaacctga ccgaagccat cgagatggcc cgcagttaca tgggccgctc cgccgagatt
     3721 acaatgctcc accagccgat catcggcatc tgcgacatga gttaagatgt gagcaaggcg
     3781 tttaagacat gagcacgacc acgaaactaa ttacggccga cgaactgctg gtgatgcctc
     3841 acccagacgc gcgcgggaac aagcgcgtct tggaactcat ccgcggggag ctgaagataa
     3901 tgtcacccac cggccttact cacgggatta tctgtacgga actcgtggtt gaattaacca
     3961 ggttcgtgaa agccaataac ctcgggatag ttgttggccc ggagacaggg tgcgtcgtcg
     4021 agcgcaaccc tgacagcgtg ctcggcgtcg atgtcgccta cgtcagccac gaacggatga
     4081 agggagtgga aaatctggaa aaatttctac ccttcgcccc cgaccttgcc gtcgaggtgt
     4141 taagcccgac caacaccgct caggagatgg acgagaaggt cgcgctctac ttcgccggcg
     4201 gcgcgggggc cgtatgggtc tttaatccga ggcggcggac tgtcgccgca tacacatcac
     4261 ctttcgatgt acgcattctg ggcgaacagg atgtgcttga cggcgggggc gttcttgaag
     4321 gattcagttt agagctgtcg aagctgttct ccgccatcaa cagttaagcc gtcttcgtgt
     4381 acagatgaaa ttatctccga cagagattta taacggccgc aacgtcttca tcatcggggc
     4441 caccggcttt ttgggcaagg tgacgctctc gatgctgttg caccgcttcc ccggcgtggg
     4501 gcgcgtctac gtcaccgtgc gcgcgcgctc gcaggaggag tcggagacgc gcttctggaa
     4561 caacgtcatc accgcgccgc ccttcgaccc gctgcgcgac ctctacggcg cggccttcga
     4621 gggcttcgtc cgcgacaagg tctcaatcgt caacggcgac atcgcgcagg acaatctcgg
     4681 gatgacggaa gaggaggcgc aggcggtcgc ctcggatatc tcggtcatca tcaacagcgc
     4741 cggcaacgtc accttcaacc cgccgctgga gagcgcgctt cggaccaacg tcgtcggcac
     4801 gaagaacgtc atcgcgtttg cgaagcggat gaagcggccg gcgctgctgc acgtctcgac
     4861 ctgcttcgtc gccggcaacc gctccggccc cgtgtgggag agcgaccccg tcgtcggcta
     4921 cttcccgcgc cgggaggaga tgccgggcgt cgagttctcc gtcgaacagg agatcaaaga
     4981 cagcgagcgt ctggccgagc gcgtgcgcga cgaggtgcgt gacgccgtga cggccgcgcg
     5041 cttccgcgag cttgcgcgca agcgtctcgt cgaagagggg cgcgacgcgg acgacctcga
     5101 cgcgatgggt cttgccgtcg cccgcgagcg caagatgtgg acgcgcgagc gcctgacgga
     5161 actcggcacc gagcgcgcac acttctgggg ctggcccaac atctacacct acaccaagag
     5221 cctcggcgag caattagtcg ccgccgagga ggggatagtt aaggccatcg tgcggccgag
     5281 catcgtcgag tcggccgccg agttcccctt ccccggctgg aacgagggct tcaccaccac
     5341 ggccccgctc atcttcatcg ccctcaaagg gcagacgctc attcccgtca acgagaaact
     5401 gatactcgac atcacgcccg tcgattcggt cgccgccttg atgctcgcgg tcgccgccga
     5461 ggcgtgcgtg cgcgagccgc gactcgtcta tcaggccgcc accggcgaca gcaatccgaa
     5521 cacgatggag cgcatcgtcg gcctgctcgg cctctacaag cgccagcact tccgcgacaa
     5581 agactcgggc ctgcgcatcg tcaacgaggt ggccgggcgg atggaaacca agcccgtcac
     5641 gccctcccgc ttcgagaaga cctcgacgcc gatggtcaac gccgcggcga agaaagtctc
     5701 cagcctgctc gacggcgtgc ggccacgttg gggcggcggc cgactcaccg agatcatcga
     5761 ccgcgcgaag gagaccgttg accgcgtcga
//
LOCUS       DASVES010000288        12504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1048541, whole genome shotgun sequence.
ACCESSION   DASVES010000288 DASVES010000000
VERSION     DASVES010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048541"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3512)
                     /gene="metH"
                     /locus_tag="VF240_11585"
     CDS             complement(<1..3512)
                     /gene="metH"
                     /locus_tag="VF240_11585"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX7175891.1"
                     /translation="MTDFLKTLKERIVVFDGAMGTNLHAQGLTVEDYGGPQFENCTEN
                     LLVTRPDAVEKVHVDFFEAGCDVVETNTFGANPIVLSEFQIAHLAYDLNVSAARMAKR
                     IAADFSTSARPRWVAGSMGPGTKLPTLGHTTYDALKASYAEQVRGLWDGGVDLFIVET
                     CQDLLQTKAALAAIFSHFERARERRPVIAQVTIEVFGTMLNGTEIGAALTALEPLAID
                     IIGMNCGTGPKHMTDSIRHLCENSPIPVSVLPNAGLPSIKDGETHYDETPESFVAQIE
                     HFARDFGVNVVGGCCGTTPEHLKMVVERMERLSPKERNARLVPSCASIYFQQPYAQDT
                     SFLIVGERVNASGSKKMRELLNAEDWDGLVSLAREQEREGAHVLDVNVDYVGRDGVAD
                     MHELISRLVTSVKIPIMLDSTEWEKMEAGLKHAGGKCILNSTNYEDGEPRYKKVLGLA
                     QEYGAGVVVGTIDEEGMARTADGKLRIARRAYEQATQELGFPAHDIFFDPLALPISTG
                     IEEDRRNALETIESLRRIKAELPGCHTILGVSNVSFGLSPASRVALNSIFLHEAVEAG
                     LDSAIVNAGKIVPLNRIGEREREVALDLIYDRRRFEGDVCVYDPLGEFTTLFAGVSTK
                     STKEDTSKLPVEERLKHHIIDGEKQNLEDNLKLALESHPALDIINEILLDGMKTVGEL
                     FGSGQMQLPFVLQSAEVMKAAVRFLEPFMEKKGGATAKGTMVLATVKGDVHDIGKNLV
                     DIILTNNGYRVVNLGIKQPIDQIISAFDEHGADAIGMSGLLVKSTLVMRDNLEILNER
                     GINAPVVLGGAALTRRYVEDDLRAVYNGGLFYARDAFDGLHLMDELTGGAGESKRNGQ
                     GASNAAAKVTTAAVVAAAAAGASALEADAGDDAEDLVGEDAKLGLRKPARPRGILDPE
                     GDTTHTVRSDVNPNVPVPAAPFYGSRVIKDISLDDVFAFVNETALFKGQWQFKQGKRS
                     AEEYRAIVEREVLPAYEELKARSKREGLLVPRVVYGYFPCQAEGNDLVIYRDDERTER
                     MRFTFPRQPAGKRLCLADFFAAKDTGRVDVVAFHLVTVGRRASEYAQELFKSDNYRDY
                     LLFHGLSVESAEALAELWHQRIREELGIAGRDAPEVTKLFHQGYQGSRFSFGYPACPN
                     LEDQTKLFELLEPERIDVELSEEF"
     regulatory      complement(3712..3787)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(3822..4289)
                     /locus_tag="VF240_11590"
     CDS             complement(3822..4289)
                     /locus_tag="VF240_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175892.1"
                     /translation="MKRSQLKVFVAAFLCLLAAASMATAGQHRARRVAVLPEVQKIYI
                     GDMGTADEADRFRLLLEEQLLKRNFAVVLKPEDADAVLTGVLAVRTYDDKSEARATVR
                     LVSQNGEVLWAKDFGHKRFTVNPFSLQEPTRRRAEEIAEALRRDFKRPTKSQP"
     gene            4509..5003
                     /gene="coaD"
                     /locus_tag="VF240_11595"
     CDS             4509..5003
                     /gene="coaD"
                     /locus_tag="VF240_11595"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX7175893.1"
                     /translation="MRRAIYPGSFDPVTNGHLDIIARGCKLFDEIIIAVVVNPGKSPM
                     FTVEERREMLEESLSEVESGSCGLVVDSFDGLLVRYAVERGAEAIVRGIRAISDYEYE
                     LQMALMNRRQEPRVETVFMMAAEDYSYVSSRLVKEVFKLGGNIDGLVPPSVEARMKAK
                     LGDV"
     gene            5105..6319
                     /locus_tag="VF240_11600"
     CDS             5105..6319
                     /locus_tag="VF240_11600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7175894.1"
                     /translation="MTSTRQPAEGKDFPVSENVGRMRASTTLAAMQAALAMRAAGVDV
                     VDLGAGEPDFDTPDHIKQAAAEAMRAGQTKYTPTAGTRKLQQAIMDYYEREFGASYQP
                     SEVMATAGGKQALFNAVVSLCGPGDEVLIPKPYWVTFPEIVNFAGATSVFIDTEETEF
                     HLTAEQVARAVTPRTKLIILNSPTNPSGRVYPQEEFKRILEGVVERRLYAITDECYLR
                     FVYPPAQVFSAATLSADIRARLCVAGSFSKTYAMTGWRIGYSLAPAAWTKHMLNVQGH
                     STSNPSSVSQAAAAAAFTGQQECVEEMLTEYRSRRDWIIGALREIPGLKCFEPEGAFY
                     AFPDVRGCFGGDVKTSGEFADKLLHDAQTVVTDGAGFGAEGYIRISYATSLERLQEGV
                     RRIRRVAESLAR"
     gene            complement(6422..7840)
                     /locus_tag="VF240_11605"
     CDS             complement(6422..7840)
                     /locus_tag="VF240_11605"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7175895.1"
                     /translation="MHDCLRHEDGLLDLIFDETGGVAARSRLLAEVERCEACGEQLRS
                     MGAALRVFDTAAESALPGENFWGGYEERLRTRMAQVIPLNGFARAGAGLLRVNARADY
                     RLTLLEDEGLARRLARQLRDVSRESQLTWPSFKEEPFGFVKRFVVAYAEFVWAFISQR
                     NVAAAAMTSFVVMLSFIGVVFGLERLRLSYMERRTPARSDYELIGMLPNNTAVPEKRE
                     EDAGSPGRARGEGGGMKPKQERPQGGGGGGREEQTRASEGKTPPGILQPQIVAPNPHP
                     PAVKNPSLAVVPHLDADAALFPPDYRPIPYGDPRSNAKELSSGPGTGDGIGDGDGGGI
                     GKGRGGGYGPGEDGNTGGGRRKIGGGGPGGPDGLRGGAGERDGVLRARDVTRRAVLVS
                     KPEPGFTEQARRNNVTGVVKLRLALRADGTVSDISVLKGLPDGLTERAIEAAKKIRFT
                     PAQREGRNASQWISVEYNFNIY"
     gene            complement(7825..8484)
                     /locus_tag="VF240_11610"
     CDS             complement(7825..8484)
                     /locus_tag="VF240_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7175896.1"
                     /translation="MNTGEDELIRRAQGGDSDAFCQIARTHQRRIYALALHYCRSPED
                     AEDLSQEVWLKAYRSVRSFRGQSSFYTWLRQITVNTFLNHQRAMTYTLDSEKTTVKME
                     ELGEADGANGAGVHHGADAEADFHQRLLVGRVMRALGELTPQQRLAFLLKHREGMTYQ
                     EIAEAFGCSAGAVKKSLFRAVSKLRESLGVGEAAAGADCVALGAGGLACPDEEATCTT
                     A"
     gene            complement(8700..10904)
                     /locus_tag="VF240_11615"
     CDS             complement(8700..10904)
                     /locus_tag="VF240_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7175897.1"
                     /translation="MNIFAFAPYLVVGFIIFVALLIILSCVLANIGAREIAIKERRYF
                     GAKMPPGRVVATEGEVGIQADVLKPGLHFIKWPFEKVVRKVPLIEIGADELGVVEAID
                     GEPMQAGSIFAPDRAQNAHNNFQDPIAFIKRGGVKGIQLRTLPPGLWPIHPYLFRVSV
                     SKATQVPPGKVGVITSADGDPLDAGRLHGKAVAHHRNFQDAEAFIGSGGQKGPQVEIL
                     TPGTYRILTQSISVENPNEMKPGLFTVRLYDATVIHENQIGLVEALDGAPLDPRDYVA
                     TPVTGHDNFQDCNEFISGGGQRGPQKDILLPGTYYINPLVFKLIPESAKEVKPGEVAV
                     IVSNVGKDPGEDIRREMANKVRERLEQEERDQVSKAAARLDKLEGENRRLNEIQEELI
                     ANDPADQRLDQGAHEAYVVPEGFRGIQESVVGPGKYYINTLAMSPIIIPTTNMTVEWT
                     AEDVDNTFDPFEVISKDGFTMKLEVRVVFRVKPEDAPFMVAKIGNTDKLIQNVMHPLI
                     DSIFRNQASESSAMAYLQNRHEEQERAEARVRMHLLKYHVDVVNVLICHIHLPEELMK
                     TQTEKILAEQRQNMYNAQREAEEKRIQLEKTRAHADNQKDLMAATVGVEISTRRSEQR
                     KAEAEGEAHYILATGKAEAEKMRLMGEAQGVAYHEQVNALGQQGVAIVETLKVIGEKG
                     VRITPDVLATGGGAGTEAGGGIGTLLLMNLFRDRIMDQQPTGNGTKTPATQK"
     gene            complement(10908..11714)
                     /locus_tag="VF240_11620"
     CDS             complement(10908..11714)
                     /locus_tag="VF240_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175898.1"
                     /translation="MKLLKAVASAGAEGTGKLSAVLIISFFLLAAISFVLYRTKRQSL
                     KSAAEQDFFPTPARGLFEDDSRGAAGGDGGGAGLASIPYTHAGDAARLRERASRGELA
                     ALGEARLTGDKKLYDEVLGALLKRDPSAENVRAVAAHVIAGRDLRASRMLAGALLTLW
                     RLSPDNVPVTDFLRVAALSDDAGVYRAAVEEVAEAWEQGRFPGRSAEDLCRLFESEYW
                     VLAPEAIRTGAGFRLKQQLAEVRRRLSAAPPPRERLSTQPDEVEAGDTLR"
     gene            complement(11890..>12504)
                     /locus_tag="VF240_11625"
     CDS             complement(11890..>12504)
                     /locus_tag="VF240_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175899.1"
                     /translation="RLTAGVWNVFLSLHGQKVAGGSRAQQLPQTALLVRAAAPEGSTQ
                     AQVARRESRLAKSLTASSSRLSATHLRAEEAFEVIGKELAMLDGLDELISVESAISEA
                     RVLVRAPKPPAAVHGHAPVSLTAPRVEALPVMFKFDKLLKGPGLKHFQERVRGEYVRR
                     AASVRTDDAAPAAPHAADYVWRVEQSQPPSRTPSCDTETEEFTF"
BASE COUNT         2019 a         4019 c         4152 g         2314 t
ORIGIN      
        1 tggaactcct cgctcagctc cacgtcgatg cgctcgggtt ccagcagctc gaagagcttc
       61 gtctgatctt cgaggttcgg gcaggccggg tagccgaaac tgaagcgcga accctgatag
      121 ccctgatgga agagcttcgt cacctccggc gcgtcgcggc cggcgatgcc cagttcctcg
      181 cggatacgct ggtgccagag ttcggcgagc gcttcggccg actcgacgct gaggccgtgg
      241 aagagaaggt agtcgcggta gttgtctgac ttgaaaagct cctgcgcgta ctcgctcgcg
      301 cgccgtccga ccgtgacgag gtggaaggcg accacgtcca cgcggcccgt gtccttcgcg
      361 gcgaagaagt cggcgaggca cagacgcttg ccggcgggct ggcgcgggaa cgtaaagcgc
      421 atgcgctcgg tgcgctcgtc gtcgcggtag atgacgaggt cgttgccttc ggcttggcac
      481 gggaagtagc cgtagacgac gcgcggcacg agcagcccct cgcgcttcga gcgcgccttt
      541 aattcctcgt aggccgggag cacttcgcgc tcgacgatgg cgcgatactc ctcggcgctg
      601 cgcttccctt gtttgaactg ccactgcccc ttgaagagcg ccgtctcgtt gacgaaggcg
      661 aacacatcgt cgagcgagat gtctttgatg acgcgcgagc cgtagaaagg ggcggccggg
      721 acgggtacgt tcgggttcac atcggagcgc acggtgtgcg tcgtgtcacc ctcggggtcg
      781 aggatgccgc gcggccgggc gggtttacgc aggccgagct tggcgtcctc gccgacgagg
      841 tcttcggcgt cgtcgcccgc gtctgcttcg agcgccgaag cgccggccgc tgctgctgct
      901 actactgcgg ctgtcgttac tttagccgcg gcgtttgacg cgccctgccc gttcctcttc
      961 gattcgccgg cgccgcccgt caattcatcc atcaggtgca ggccgtcgaa ggcgtcgcgg
     1021 gcgtagaaga gaccgccgtt ataaacggcg cgcaggtcgt cctcaacata gcggcgcgtg
     1081 agcgcggcgc cgccgaggac gacgggcgcg ttgatgccgc gctcgttgag gatttcgagg
     1141 ttgtcgcgca tgacgagcgt ggatttgacg agcaatcccg acatgccgat ggcgtcggcg
     1201 ccgtgctcgt cgaaggcgga gatgatctgg tcgatgggct gcttgatacc gaggttgacg
     1261 acgcggtagc cgttattcgt caggatgatg tccacgaggt tcttgccgat gtcgtgaacg
     1321 tcgcccttga cggtggcgag caccatcgtc cccttcgccg tcgccccgcc cttcttctcc
     1381 atgaagggtt cgaggaagcg cacggccgcc ttcatcacct cggccgactg caagacgaac
     1441 ggcagttgca tctggccgct gccgaagagt tcgcccacgg tcttcatgcc gtcgagcagg
     1501 atttcgttga tgatgtcgag cgccgggtga ctctccagcg cgagcttgag attgtcttcg
     1561 agattctgct tctcgccgtc gatgatgtga tgcttcaggc gctcctctac gggcagcttg
     1621 ctcgtgtcct ctttcgtgct cttcgtggag acgcccgcga agagcgtggt gaactcgccg
     1681 agcgggtcgt agacgcacac gtcgccctcg aagcggcggc ggtcgtagat gaggtcgagc
     1741 gccacttcgc gctcgcgctc gccgatgcgg ttgagcggga cgatcttgcc ggcgttgacg
     1801 atggccgaat caagcccggc ctcgacggcc tcgtgcagaa agatggagtt gagcgcgact
     1861 cgcgaggcgg gcgagagtcc gaaggagacg tttgagacac cgaggatggt gtgacaaccg
     1921 ggcagctcgg ccttgatgcg gcgcagcgac tcgatggttt cgagcgcgtt gcgacgatcc
     1981 tcttcgatgc cggtcgagat ggggagcgcg agcgggtcga agaagatgtc gtgcgcgggg
     2041 aagccgagtt cctgagtcgc ttgctcgtag gcgcggcggg cgatcctgag cttgccgtcg
     2101 gccgtgcgcg ccatgccctc ctcgtcaatc gtcccgacga cgacgcccgc gccgtactcc
     2161 tgcgccagcc ccaaaacctt tttgtagcgc ggctcgccgt cctcgtagtt cgtcgagttg
     2221 aggatgcact tgccgccggc gtgtttgagg cccgcctcca tcttctccca ctcggtggag
     2281 tcgagcatga tcgggatttt gacgctggtg acgagccgcg agatgagttc gtgcatgtcg
     2341 gcgacgccgt cgcggccgac gtaatcgacg ttgacatcga ggacatgcgc gccctcgcgc
     2401 tcctgctcgc gcgcgagcga gacgagaccg tcccagtcct cggcgttcaa aagctccctc
     2461 atcttcttcg accccgaggc gttgacgcgc tcgccgacga tgaggaaaga agtgtcctgc
     2521 gcgtaaggct gctggaagta gatggaagcg cacgacggga cgagccgcgc gttgcgctcc
     2581 ttcggcgaca gccgctccat ccgctcgacg accatcttga gatgctccgg ggtggtgccg
     2641 cagcaaccgc cgacgacgtt gacgccgaag tcgcgcgcga agtgttcgat ctgcgcgacg
     2701 aaggattcgg gcgtctcgtc gtagtgcgtt tcgccgtcct tgatcgaggg aaggccggcg
     2761 ttcgggagca cggagacggg gatgggcgag ttctcgcaga ggtgccggat actgtcggtc
     2821 atgtgcttcg gcccggtgcc gcagttcatg ccgatgatgt cgatggcgag gggttcgagc
     2881 gcggtgagcg cggcgccaat ctcagtaccg ttgagcatcg tgccgaagac ttcaatcgtc
     2941 acctgcgcga tgacgggccg gcgctcacgc gcccgctcga agtgagagaa gatggccgcg
     3001 agcgccgcct tcgtctggag caaatcctga caggtctcga cgatgaagag atcgacgccg
     3061 ccgtcccaca gcccgcgcac ctgctcggca taagacgcct tgagcgcgtc gtaggtggtg
     3121 tggccgagcg tggggagctt cgtgcccggc cccatcgagc cggcgaccca gcgcgggcgc
     3181 gcgctcgttg agaagtcggc ggcgatgcgt ttggccatgc gcgcggccga cacgttgagg
     3241 tcgtaggcca ggtgggcgat ctggaactcg gagagcacga tggggttcgc gccgaacgtg
     3301 ttcgtctcga ccacgtcgca gccggcctcg aagaagtcca cgtgtacctt ttcgaccgcg
     3361 tcggggcgcg tgacgagcag gttctccgtg cagttctcga actgcggccc gccgtagtcc
     3421 tccaccgtca acccctgcgc gtgcaggttc gtccccatgg caccgtcgaa aacgacgatg
     3481 cgctctttta gtgtcttgag aaaatcggtc ataaggaagt tgtcagtaat cagctgtcag
     3541 tagtcagttg tttccgttcc ccaacttgaa cctttcaaag tcagcggcga gtagcccgag
     3601 acaaaaaact gactactgac aactgactac tgactactat aaaaagaagc cccgcgccaa
     3661 acaacgcggg gccttgacgt tcggaagcct tttaaacgtc ggtctctcct tcgagggacg
     3721 cggccgagct gtttagcggt ttattttacg tggccgcaag tcgccttaac tcaccacgtt
     3781 tgtctacggt caaacattga aacatcgccc gcgcgggcgt gtcaaggctg gctcttggtg
     3841 ggcctcttga agtcgcggcg cagcgcctcg gctatctcct cggcgcgccg gcgggtcggc
     3901 tcctggaggc tgaacgggtt gacggtgaac cgcttgtggc cgaagtcctt cgcccacagc
     3961 acctcgccgt tctgtgagac gagccggacg gtggcgcgcg cctccgactt gtcgtcgtaa
     4021 gtacgtaccg cgagcactcc ggtcaggacg gcgtcggcgt cctcgggctt gagtacgacg
     4081 gcgaagtttc ttttgaggag ctgttcttcg agcagcaggc ggaagcggtc ggcctcgtcg
     4141 gccgtgccca tgtcaccgat ataaatcttt tgcacttcgg ggagcaccgc cacccggcgg
     4201 gcgcggtgct ggccggcggt ggccattgac gcggcggcca gcaggcacag gaaggccgcg
     4261 acgaatactt tcagttgcga gcgtttcatc tttttctcct ctcacgggta acggggcttc
     4321 gggacggtta catttgataa cggggagggc cacccccggc gaggctacgg ttttttgcgg
     4381 tctgcccttt gaaggccggc gcgcccgcgc gcgttggccg aaataatcaa aacttcaccg
     4441 ggcgagcgct tttgtgttaa cttcgccgct gacttctcat cccgtacaca ggtcggacac
     4501 gaacgcctat gcgccgcgcc atctatcccg gttcgttcga ccccgtgacc aacggccacc
     4561 tcgacatcat cgcgcgcggc tgcaagctct tcgacgagat catcatcgcc gtcgtcgtca
     4621 accccggcaa gagtcccatg ttcaccgtcg aggagcggcg cgagatgctc gaagagtcgc
     4681 tatccgaggt cgagtccggc tcgtgcggcc tcgtcgtcga ttccttcgat ggcctgctcg
     4741 tccgctacgc cgtcgagcgc ggggcggagg cgattgtgcg cggcatccgc gccatctcgg
     4801 attacgagta cgagttgcag atggctttga tgaaccggcg gcaggagccg cgcgtcgaga
     4861 ccgtcttcat gatggccgcc gaggactact cctacgtcag ctcgcggctc gtcaaggagg
     4921 tcttcaaact cggcggcaac atcgacggcc tcgtgccgcc gtcggtcgag gcgcgcatga
     4981 aggcgaaact cggggacgtt tgaaagtcgg cgcgcccatg tgggccatgc gtctcgcttg
     5041 cacacgcggg cgcgacgcgt ttaccatctc gcgtcaacaa tccacagaag actgaggtca
     5101 accgatgacc agcacgcggc agccggccga aggcaaagat tttcccgtct cggagaacgt
     5161 cggacggatg cgcgcctcca cgacgctcgc cgccatgcag gcggcgctgg ccatgcgcgc
     5221 ggccggagtt gacgtggtcg atctcggcgc gggtgagcct gacttcgaca cgcccgacca
     5281 catcaagcag gccgccgccg aggcgatgcg cgccggtcag acgaagtaca cgccgaccgc
     5341 cggcacacgc aaacttcagc aggccatcat ggactactac gagcgcgagt tcggcgcgag
     5401 ttatcagcct tcggaagtga tggcaacggc cggcggcaag caggccctct tcaacgccgt
     5461 ggtctcgctc tgcggccccg gcgacgaggt tctgattccg aagccctact gggtgacctt
     5521 ccccgagatc gttaacttcg ccggcgccac ttccgtcttc atcgacaccg aggagacaga
     5581 gtttcacctg acggccgagc aggtcgcgcg agccgttacg ccgcgcacca agctcatcat
     5641 cctcaactcg ccgacgaacc cctcggggcg cgtctatccg caggaagagt ttaagaggat
     5701 tctggaaggg gtcgtcgagc gccgtctcta cgccatcacg gacgagtgtt atctacgctt
     5761 cgtctacccg ccggcgcagg tattcagcgc ggcgaccctg tccgccgaca tacgcgcgcg
     5821 cctgtgcgtc gccggctcct tctcgaagac ctacgcgatg accggctggc gcatcggcta
     5881 ttcgctcgcg cccgccgcct ggacgaagca catgctcaac gtgcagggcc actcgacgag
     5941 caatccgtcg tcggtctcgc aggcggcggc cgccgcggcc ttcacggggc agcaagaatg
     6001 cgtcgaggaa atgctcacag agtatcgcag ccggcgcgac tggatcatcg gggcgctgcg
     6061 cgagataccg ggcctcaagt gcttcgagcc ggagggcgcc ttctacgcct tccccgacgt
     6121 gcgcggctgt ttcggtggcg acgtgaagac ctcgggtgag ttcgccgata aacttttgca
     6181 cgacgcgcag acggtcgtca ccgacggcgc gggcttcgga gccgagggct acatccgcat
     6241 ctcctacgcg acctcgctcg aacgattgca ggagggcgtc cgccgtatcc gccgcgtcgc
     6301 ggaatcactc gcgcggtaag ctgccggtag acacagtacc gcgagcggta gcgagcgggt
     6361 ctacttacac gagtagaccc gctcgctacc gctcgcggta ctgttgtgtt gtgattccgg
     6421 gtcagtagat gttgaaattg tactcgacac ttatccactg agaggcgttc cgcccctccc
     6481 tctgcgcggg ggtgaagcga atcttcttcg ccgcttcaat cgcccgctcc gtcaacccat
     6541 cgggcagacc ctttaagacc gagatgtccg agaccgtgcc gtcggcgcgc agggcgaggc
     6601 ggagtttgac cacgcccgtg acgttgttgc gccgcgcctg ctccgtgaag cccggctccg
     6661 gcttggagac gaggacggcg cggcgcgtca catcgcgcgc ccttagaacg ccgtcgcgtt
     6721 cgcccgcgcc gccgcgcaac ccgtcggggc cgccggggcc gccgccgccg atcttgcgcc
     6781 ggccgccgcc cgtgttgccg tcttcgccgg ggccgtaacc gccgccgcgg cctttaccga
     6841 tgccgccgcc atcgccatcg ccgatgccgt cgcccgtgcc ggggccggat gacaactcct
     6901 tcgcgttaga gcgcgggtcg ccgtagggga tgggccgata gtcgggcggg aagagtgcgg
     6961 cgtcggcgtc gaggtgcggc acgacggcga gactcgggtt cttcaccgcc ggcgggtgcg
     7021 ggtttggcgc gacgatctgc ggctgcaaga taccgggcgg ggtcttcccc tccgacgcgc
     7081 gcgtctgctc ttcgcggccg ccgccgccgc cgccctgcgg acgctcctgc ttcggcttca
     7141 tgccgccgcc ctcgcctctg gcccggccgg gcgagccggc gtcctcctcg cgcttctcag
     7201 ggacggccgt gttgttgggc agcatcccga tgagttcgta gtcgctgcgc gcgggcgtcc
     7261 gtcgctccat gtaggacaga cggagtcgtt cgagtccgaa gacgacgccg atgaacgaca
     7321 gcatgacgac gaaggaggtc atggccgccg cggcgacgtt gcgttgcgag atgaatgccc
     7381 agacgaactc ggcgtaggcc acgacgaagc gtttgacgaa gccgaacggc tcctccttga
     7441 acgagggcca cgtgagctgc gactcgcgcg acacgtcgcg cagttgccgc gccagacgcc
     7501 gcgccaagcc ctcatcctca agaagcgtca aacgatagtc ggcgcgcgcg ttcacgcgca
     7561 gtaagcccgc gcccgcccgc gcgaatccgt ttagcgggat gacttgtgcc atgcgagtcc
     7621 tcaatctttc ttcgtagccg ccccagaaat tctcgccggg gagcgccgac tccgcggccg
     7681 tgtcgaacac acgcagcgcc gcccccatcg agcgcaactg ctctccgcac gcctcgcacc
     7741 gctcgacctc cgcgagcaga cgcgaacgcg ccgccacgcc gcccgtctcg tcgaagatta
     7801 ggtcgagcag gccgtcttcg tgtctcaggc agtcgtgcat gtcgcctcct catcgggaca
     7861 ggcaagcccg cccgcgccga gcgcgacgca gtcggccccc gccgcggcct cgcccacacc
     7921 caacgactcg cgcagcttcg agacggcgcg gaagagcgac ttcttgaccg cgcccgccga
     7981 gcagccgaac gcctcggcga tctcctgata ggtcatcccc tcgcggtgct tgagcaggaa
     8041 ggcgagacgc tgctgcggag tcagctcgcc gagcgcgcgc atgacgcgcc cgacgaggag
     8101 gcgctgatga aagtcggctt cggcgtcggc tccgtgatgc acgcccgcgc cgttcgcgcc
     8161 gtcagcctcg ccgagttctt ccatcttcac ggtcgtcttc tcgctgtcga gggtgtaagt
     8221 catggcgcgc tggtggttga gaaaagtgtt gacggtaatc tgccggagcc atgtgtagaa
     8281 gctcgattgg ccgcggaagc ttcggacgct gcggtaagcc ttgagccaga cctcttgcga
     8341 gaggtcttcg gcgtcttcgg ggctgcggca gtagtggagg gcgagcgcgt agatgcgccg
     8401 ctggtgcgtc cgcgcgatct ggcagaaggc atccgagtcg cccccctgcg cccgcctgat
     8461 taactcgtct tcgcctgtgt tcacaatcaa gccctttgcg acggtcgcag atatagacac
     8521 ggctcgcgcg cggaaggttg gcgggagagg cggaagtttt tatagatcaa tttttaccag
     8581 cgtgtgatgc gcggcagact cacactccac aaacagttcc ctcttccaga caaaaaaatc
     8641 ggccaccccg tttccgggat ggccgacgcg cacgcgaaag tgtggatgcg ttcgcgggat
     8701 tacttctgcg tggccggcgt cttcgtgccg ttgccggtcg gctgctgatc catgatgcgg
     8761 tcgcggaaga ggttcatcag cagaagtgtg ccgatgccgc cgcccgcctc cgtgccggcg
     8821 ccgccgcccg tcgccaacac gtcgggagtg atacggacgc ccttctcgcc gatgaccttc
     8881 agggtctcga cgatggcgac gccctgctgc ccgagcgcgt tgacctgctc gtggtaggcg
     8941 acgccctgcg cctcgcccat cagacgcatc ttctcggcct cggccttacc cgtggcgagg
     9001 atgtagtggg cctcgccctc ggcctcggcc ttgcgctgct cggagcgccg ggtcgaaatc
     9061 tcgacgccga cggtcgccgc catcaggtct ttctggttgt cggcgtgggc gcgcgtcttc
     9121 tcaagctgga tgcgcttctc ctcggcctcg cgctgcgcgt tgtacatgtt ctggcgctgc
     9181 tcggcgagaa tcttctcggt ctgcgtcttc atcaactcct cggggagatg gatgtggcag
     9241 atgaggacgt tgaccacgtc aacgtgatac ttcagcaggt gcatccgcac gcgcgcctcg
     9301 gcccgctcct gctcctcgtg gcggttctgg agataagcca tcgcggacga ctccgacgcc
     9361 tgattgcgga agatggagtc gatgagcggg tgcatcacgt tctggatgag cttgtcggtg
     9421 ttgccgatct tcgccaccat gaagggcgcg tcttcaggtt tgacgcggaa gacgacgcgc
     9481 acttcgagct tcatcgtgaa gccgtctttg gagatgacct cgaaggggtc gaaggtgttg
     9541 tccacgtcct cggccgtcca ctccaccgtc atgttcgtcg tcgggatgat gatcggcgac
     9601 atggccagcg tgttgatgta gtacttgccg gggccgacga ccgactcctg tatgccgcgg
     9661 aagccttcgg gcacgacgta agcctcgtgc gcgccctgat cgagccgctg gtcggccggg
     9721 tcgttggcga tgagttcttc ctgaatctcg ttgaggcggc ggttctcgcc ttcgagcttg
     9781 tcgaggcggg cggcggcctt cgagacctga tcgcgctcct cctgttcgag ccgctcgcgc
     9841 accttgttgg ccatctcgcg gcggatgtcc tcgccggggt ctttgccgac gttcgagacg
     9901 atgacggcca cctcgcccgg cttcacttcc ttcgccgact cggggatgag cttgaagacc
     9961 agcgggttga tgtagtaggt gccgggcagg aggatgtcct tctgcggccc gcgctggccg
    10021 ccccccgaga tgaactcgtt gcagtcctgg aaattgtcgt ggccggtgac cggggtggcg
    10081 acgtagtcgc gcgggtcgag cggcgcgccg tccaaagcct cgacgagtcc gatctggttc
    10141 tcgtgaatca cggtcgcgtc gtagaggcgg acggtgaaca gccccggctt catctcgttg
    10201 ggattctcaa ccgagatcga ctgcgtaagg atgcggtagg tgccgggcgt caggatttcg
    10261 acctgcggcc ccttctggcc gccgctgccg atgaaggctt cggcgtcctg aaagttgcgg
    10321 tggtgggcga cagccttgcc gtggaggcgg ccggcgtcga gcgggtcgcc gtcggccgag
    10381 gtgatgacgc cgaccttgcc cggcggcacc tgcgtcgcct tcgagacgga gacccgaaac
    10441 aggtacgggt gaatcggcca caggcccggc gggagcgtgc gcagttggat gcccttgacg
    10501 ccgccgcgtt tgatgaacgc gatggggtcc tggaagttgt tgtgggcgtt ctgcgcgcgg
    10561 tcgggggcga agatactccc ggcctgcatc ggctcgccgt caatcgcctc gacgacgccg
    10621 agttcgtccg ccccgatctc gatgagcggg actttgcgga cgactttctc aaagggccac
    10681 ttgatgaagt ggaggcccgg cttgagcacg tcggcctgaa tgccgacctc gccctcggtg
    10741 gcgaccacgc ggccgggcgg catcttcgcg ccgaagtagc gccgctcctt gatcgcgatc
    10801 tcgcgcgcgc cgatgttggc gagcacgcag ctcaagatga tgagcagggc cacgaagata
    10861 atgaacccga ccaccaggta gggggcgaaa gcaaaaatgt tcatggttta cctcagagtg
    10921 tcgccggctt cgacttcatc cggctgtgtg gagagacgct cgcgcggtgg cggcgcggcc
    10981 gacagacggc gacgcacctc ggcgagttgt tgcttgagac ggaagccggc gcccgtgcgg
    11041 atggcctcgg gcgcgagcac ccagtattcg ctctcgaaga gacggcacag gtcttcggcc
    11101 gacctgccgg ggaagcgtcc ctgctcccag gcttcggcga cctcctcgac cgccgcgcga
    11161 taaactcccg cgtcgtcgga gagcgcggcg acgcgcagaa agtctgtgac cgggacgttg
    11221 tcgggcgaaa gtctccagag ggtcaacagt gcgccggcga gcatccggct ggcgcgaagg
    11281 tctcggcccg ctatcacgtg cgcggcgacg gcgcgcacgt tctcggccga ggggtcgcgc
    11341 ttgaggagcg cgccgagcac ttcgtcgtag agttttttgt cgcccgtcaa gcgggcctcg
    11401 ccgagcgccg ccagctcccc gcggctggcc cgctcgcgca agcgcgcggc gtctccggcg
    11461 tgtgtgtagg gaatagaggc aaggcccgcg cccccgccgt cgccgcccgc cgcgccgcgc
    11521 gagtcgtctt caaaaagccc cctcgcaggc gtggggaaga agtcctgctc ggccgcgctc
    11581 ttgagcgatt ggcgtttcgt gcgatagagg acgaaagaga tagctgccag aaggaagaac
    11641 gaaatgatga gaacggcgct caatttacct gtgccttccg cgccggccga cgcgacggct
    11701 ttcaacaatt tcaagccggt gctaccggac gccgttgcat cagcattata cgacaggggg
    11761 gtcggaaagt tctacggcca cgggcgggaa gcaccaaagc cccgcacgtc cccaaagggg
    11821 agacgtgcgg ggcacattga atgaagcgcg aaagatttcc cgagtttacg ggacgctcac
    11881 cggggtgttt taaaacgtga actcttccgt ctcggtgtca cacgaaggcg tgcggctcgg
    11941 cggctggctc tgctcgaccc gccagacata gtcagcggcg tgcggcgccg ccggtgcggc
    12001 gtcgtccgtc ctgaccgacg cggcccggcg cacgtactcg cccctgacgc gctcctggaa
    12061 atgtttgagg cccggcccct tgaggagctt gtcgaacttg aacatgacgg ggagtgcttc
    12121 gacgcgcggc gccgtcaggg agacgggggc gtggccgtgc accgcggccg gcggttttgg
    12181 ggcgcgtacc agaactcgcg cctctgaaat cgccgactcg accgagatca actcgtcgag
    12241 gccgtcgagc atcgccagct ctttcccgat cacttcaaac gcctcttcgg cgcggagatg
    12301 cgtggcgctc agccgagacg acgatgccgt caacgactta gccaggcgcg actctctcct
    12361 cgcgacctgc gcctgtgtgg agccttccgg cgcggcggcg cggacgagga gcgccgtctg
    12421 cgggagttgc tgcgcgcgcg acccgcccgc gaccttctgc ccgtggagac tcaggaaaac
    12481 gttccagaca cccgcggtca gccg
//
LOCUS       DASVES010000289         7130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1176550, whole genome shotgun sequence.
ACCESSION   DASVES010000289 DASVES010000000
VERSION     DASVES010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176550"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF240_11630"
     CDS             <1..272
                     /locus_tag="VF240_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX7175900.1"
                     /translation="QLIGEKEFPTLSHEGERESIHICIIGRMLLVVVFDDRSSLGLVK
                     LRVKQASRELAIIFEDVERDSLQKREQDDSLFAGITDDDIDSLFN"
     gene            343..927
                     /locus_tag="VF240_11635"
     CDS             343..927
                     /locus_tag="VF240_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX7175901.1"
                     /translation="MTFINYASREINCKIVYYGPGLCGKTTNLQYIYDSTAPASKGKL
                     ISLATETDRTLFFDFMPLELGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQEERMDANIESLYNLEENLQANGYDLTKIPYVLQLNKRDLPNVVPIEDLVAELRR
                     KDEPVHEAVAFKGPGVFDTLKAVAKQVLTELRKS"
     gene            complement(1028..1606)
                     /locus_tag="VF240_11640"
     CDS             complement(1028..1606)
                     /locus_tag="VF240_11640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7175902.1"
                     /translation="MAVLKKIRSRLFSASGTSAPGAEDDDAAAAAGETRSLGLVARPR
                     YDIRPLTTAQLDECWRLDLRCFIDGEAYSRETFEYLLTSPDSVSYRAVTPDGAMVGFI
                     VGLVEPDSTGHITTLGVAPEHRRRHIAHRMMENAEQGFRRREVRTVRLEVRSINDSAQ
                     LLYAGLGYVVTQRLPRYYSNGGDGLLMIKSLV"
     gene            complement(1717..2781)
                     /gene="queA"
                     /locus_tag="VF240_11645"
     CDS             complement(1717..2781)
                     /gene="queA"
                     /locus_tag="VF240_11645"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7175903.1"
                     /translation="MLISAFDYELPEELIAQHPAERRDASRMLVVERSSGTWRDSHFS
                     ELPAELRAGDCLVVNNTRVFPARLMGRRVPSGGRVELLLVREMVERGPGVWEALTRPA
                     RRLEVGARVEFGGGDSSRLVAEVVESFADGRRALRFEPAGDFDALLEELGEMPLPPYI
                     RREGQPDAERAEDRERYQTMFAAPRGAIAAPTAGLHFTPHVAEHLRARGLSIVELTLH
                     VGYGTFAPVRVKDLSEHRVEPERFEVGEEAADNINRARSGGGRVVAVGTTSVRALESA
                     ADAQGRVRAGRGETGLTITPGREFRAVDALFTNFHLPRSSLLVLVAAFAGRELILNAY
                     AHAVRARYRFYSYGDCMLIS"
     gene            complement(2906..3817)
                     /locus_tag="VF240_11650"
     CDS             complement(2906..3817)
                     /locus_tag="VF240_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7175904.1"
                     /translation="MQRATFFSRHRWGVRNRAPWTTILSHTLHQFHENDLFTPSAAMS
                     YFGLLTLFPALILFLALGNRLAAGSEMLRQIVEVYPGSAEFLRSNVRSLTGVSTGAIV
                     SCFVVVLWAGSWVFAVVERALNRLWGTQPRAFLHGRALTLGMIGLVGCVLLVSVFLTS
                     ILLGLEQLAEQLPIRIVSRSRWLALVGSGIWQTVYLLASTLVTVAVFALVYRLVPSGR
                     VTLRDTLPSAVVAGILWETSKYVFAWSLQYFHYDQIYGSVGAVVAILTWSYVSSLILM
                     FGAQLSVVLHQEHHAAELAAETPASPS"
     gene            complement(3884..5911)
                     /locus_tag="VF240_11655"
     CDS             complement(3884..5911)
                     /locus_tag="VF240_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175905.1"
                     /translation="MPDKSNKLRCPVALFAACVVVAPCVASTRRHEGVCAPAASVPPQ
                     QTTGEGARASGQNTRKRGGQSAAARASAAREKQRAVSALLEAADAARVFDDRFYSAKV
                     QALAADALWLADERAARALFRRAWEAATEADLAEMKEMAAESGEDGNSPHLFTDARDV
                     VISAATRRDPRLGETFFGELMKSQSELGSDPRRSAQTSYAAGPSEGVSVEGWQRLQLA
                     ERLLAEGDPQRAAALAAPVVAEGPSIDLVHFLLELRAHAPTEADDLFLRLLQRMRGDA
                     SADSGDVLLLSSYILTPNLLTTIDSHGTVNFRNLSFKNPAAEPPPSPRVLVAFFDTAA
                     GVLLRSAAPSPDADEVSAESKLLGLFFTTRRLLPFFEREAPRHAPQLHARLQSLAADI
                     DAERRGHLSAQADVRKVTSNNSTDRLGPTLDAISKADEGPARDRLRLQAVHRAAELKM
                     WERAQSFADSITQPEARRKALLLVTVYQLLSLRGAYAEGEEQDFELAASFVRGADAPP
                     LALAFGMAQAAELAAKRNKPERANELLDQASAHAGRAAQGGEQRAVAFAVVAITAASI
                     DAARAWDALSEFVRAANAFDEYVGDEISFRSDANSEVLDGDYLNAIRAQLSTFDLTKV
                     FTTVARLDFGRALEQARALEGREARAFATVAAARARLERDTRGMRGAGEKQ"
     gene            complement(5936..6982)
                     /gene="ruvB"
                     /locus_tag="VF240_11660"
     CDS             complement(5936..6982)
                     /gene="ruvB"
                     /locus_tag="VF240_11660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7175906.1"
                     /translation="MDELEPVSVREQGAPDEERQFELSLRPAHLAEYIGQKKVTENLR
                     VFISAARKRRESLDHVLLSGPPGLGKTTLGNIIATEMGAELRSTAGPVIEKPGDLAAM
                     LTNLQEGDVLFIDEIHRLHPAIEEVLYPAMEDYQLDLMIGQGTAARSIKLDLPKFTLV
                     GATTRAGLITAPLRGRFGIVFHLTVYAHDELELIVRRSAEILGTEIDDDGAREIARRA
                     RGTPRIANRLLRRVRDFAEVEHDGRITKVVAGDALNRMEVDTYGLDEVDRKLLLSIID
                     QHHGGPVGLSTLSAMLHEEKESIEELIEPYLIQIGFLNRTPRGRVATRLAYEHFGLPP
                     TAAPPRPGALFESE"
BASE COUNT         1280 a         2225 c         2442 g         1183 t
ORIGIN      
        1 cgcaactcat tggcgaaaaa gagtttccga cgctgtccca cgaaggcgag cgcgagagta
       61 tccacatttg catcatcggg cggatgctgc tcgtcgtcgt ttttgacgac cgttccagtc
      121 tcgggctggt taaattgcgt gtgaaacagg cctcgcgcga actggctata atttttgagg
      181 atgtcgaacg cgattcgttg caaaagcggg agcaggacga ctcgctcttc gccggcatca
      241 cggatgatga cattgacagt ttattcaact aaccaaggtt aacagaggcg accggcgaaa
      301 gtgacaagtt agaacttgtg cgggcctgtc attccaatcg ctatgacgtt tatcaattac
      361 gcatcacgcg aaatcaattg caagatcgtc tattacgggc cggggctgtg cggcaaaacg
      421 accaacctgc aatacatcta cgactcgacc gcgcccgcct cgaaaggcaa actcatcagc
      481 ctcgcgaccg aaaccgaccg cacgctcttc ttcgacttca tgccgctcga actcggcacg
      541 gtgcgcggct tcaagacgcg cttccacctc tacaccgtgc cgggccaggt cttctacgac
      601 gcctcgcgca agctcatcct gaagggcgtg gacggcgtcg tcttcgtcgc cgactcgcag
      661 gaggagcgca tggacgccaa catcgagtcg ctctacaacc tcgaagagaa cctccaggcc
      721 aacggctacg acctcacgaa gattccctac gtgttgcagc tcaacaagcg cgatttgccg
      781 aacgtcgtgc ccatcgaaga cctcgtcgcc gaactcaggc ggaaggacga gccggtccac
      841 gaggccgtcg ccttcaaggg gccgggcgtc ttcgacactc tgaaggcggt cgccaagcag
      901 gtgctcacgg aactgcggaa gagctagacg ggtaagagcg cgtaaatctg aaagcgcgta
      961 aatcgaaaat cgtgaatcgt caatcgaagc aggcagtttc gattgacgat tcacgattta
     1021 cgcgctctta aactaatgac ttgatcatca ggaggccgtc gccgccgttg gagtagtagc
     1081 gtgggaggcg ctgggtgacg acgtagccga ggccggcgta gagaagctgc gccgagtcgt
     1141 tgatggagcg gacttccaag cggacggtgc gtacctcgcg gcggcggaat ccctgttcag
     1201 cattctccat catgcggtgg gcgatgtggc ggcggcggtg ctcgggggcg acgccgagcg
     1261 tggtgatgtg gccggtcgag tccggctcga cgaggccgac gatgaagccg accatcgcgc
     1321 cgtcgggcgt gacggcgcgg taggagacgg agtcgggcga ggtcaggagg tattcgaagg
     1381 tctcgcggct gtacgcctcg ccgtcgatga agcagcgcag gtcgagccgc cagcactcgt
     1441 cgagctgcgc ggtcgtcagc ggccgaatgt cgtaacgcgg ccgcgcgacg aggccgagcg
     1501 agcgcgtctc gccggccgcc gcggccgcgt cgtcatcctc tgcgccaggt gcggacgtgc
     1561 cactcgccga gaacagccgc gaacgtatct tcttcaggac agccactcgt aaatcgtaaa
     1621 tcgtcaatcg taaatagtaa atacccagac gccgtctttc tccgattcac gacttccgga
     1681 tcgcgattcg cgattcacga ttgacgattt acggctttaa cttatgagca tgcagtcgcc
     1741 gtagctgtag aagcggtagc gggcgcggac ggcgtgggcg taggcgttga ggatgagttc
     1801 gcgcccggcg aaggcggcga cgaggacaag gagcgacgag cggggcaggt ggaagttcgt
     1861 gaagagcgca tcgacggcgc ggaactccct accgggcgtg attgtgaggc cggtctcccc
     1921 gcggccggcg cgcacccgcc cctgagcatc ggccgctgac tctaacgcgc ggacgctcgt
     1981 cgtcccgacg gcgacgacgc gtccgccccc gcttcgcgcg cggttgatgt tgtcggccgc
     2041 ctcttcgccc acctcgaaac gctccggctc gacgcggtgc tcgctcaagt ctttaacgcg
     2101 cacgggcgcg aaggtgccgt agccgacgtg cagcgtcagc tcgacgatgg agaggccgcg
     2161 cgcgcgtaag tgttcggcaa cgtgcggcgt gaagtggagg ccggcggtcg gggcggcgat
     2221 agcgccgcgc ggggccgcaa acatcgtctg gtatcgctcg cgatcctcgg cgcgctcggc
     2281 gtccggctgt ccttcgcgcc ggatgtaggg cggaagcggc atctcgccca gctcctcgag
     2341 aagcgcgtcg aagtctccgg ccggctcgaa gcgcaaggcg cgccgcccgt cggcgaacga
     2401 ctcgacgacc tccgcgacga ggcgactgct gtcgccgccg ccgaactcga cccgcgcgcc
     2461 gacctctaac ctccgcgcgg gccgcgtcag agcctcccac acgcccggcc cacgctcgac
     2521 catctcgcgc accagcagca gctcgacgcg cccgcccgag ggcacgcgcc gccccatgag
     2581 acgcgccggg aagacgcgcg tgttgttgac caccagacag tcgcccgcgc gcaactccgc
     2641 aggcagctcc gagaagtgac tatcgcgcca cgtccccgag gagcgttcga cgaccagcat
     2701 ccgcgaggcg tcgcgccgct cggccgggtg ctgcgcgatc agttcttcgg gcagctcgta
     2761 gtcgaaagcg gaaatcaaca tgaaggcgaa ggaagtttac atggatggac aggacaggca
     2821 ggatcagaag atttgaaatc tcaaatctga aatttcaaat catcctttct tcatcctgta
     2881 tgtcctgtcc atccatgtaa atattctaag acggagaggc cggcgtctcg gccgccagct
     2941 ccgcggcgtg gtgctcctgg tggaggacga ccgagagttg cgcgccgaac atcaggatca
     3001 ggctcgacac gtagctccac gtcaggatgg cgacgacggc gcccaccgag ccgtaaatct
     3061 ggtcgtagtg aaaatactgg aggctccacg cgaagacgta cttcgaggtc tcccatagta
     3121 tcccggccac gaccgcgctc ggcagcgtgt cgcgcagcgt gacgcgcccg ctcggcacca
     3181 gccggtagac gagcgcgaag acggcgaccg tgacgagcgt gctcgccagc aggtagacgg
     3241 tctgccagat gccgctgccg accagcgcca gccagcgcga gcggctgacg atgcggatgg
     3301 gcaattgctc ggccagttgc tcaagcccca ataggatcga cgtgaggaag accgagacga
     3361 gcagcacgca gccgacgagg ccgatcatcc cgagcgtgag cgcgcggccg tggaggaagg
     3421 cgcgcggctg cgtcccccac aggcggttga gcgcgcgctc gacgacggcg aagacccacg
     3481 agccggccca cagcacgacg acgaagcaac tgactatcgc gcccgtcgag acgcccgtga
     3541 gcgagcgcac gttcgagcgc aaaaactcgg ccgagccggg gtagacctcg acgatctgcc
     3601 gcagcatctc gctgccggcg gcgaggcggt tgccgagggc gaggaagaga atcagcgcgg
     3661 ggaagagcgt gagcagcccg aagtagctca tggccgcgga gggcgtgaag aggtcgttct
     3721 cgtggaactg gtggagggtg tggctgagga tggtcgtcca cggtgcgcgg ttgcgaacgc
     3781 cccacctgtg ccgtgagaaa aaggtagccc tctgcatgat cgctcggaag cccgaaggga
     3841 agactcgaaa actgttgctc ggtcgggtgg cccgagcgtc agtctactgc ttttcgcccg
     3901 ccccgcgcat cccgcgcgtg tcgcgttcga gtcgcgcgcg ggcggcggcg accgtggcga
     3961 aggcgcgcgc ctcacggcct tcgagcgcgc gcgcctgctc cagtgcgcgg ccgaagtcga
     4021 gccgcgcgac cgtggtaaaa actttcgtca ggtcgaaggt ggagagctgc gcgcggattg
     4081 cgttcaggta gtcgccatcg agaacttcgg agttggcgtc ggagcggaag ctgatttcgt
     4141 cgccgacgta ttcgtcgaac gcgttcgcgg cgcggacgaa ctccgagagc gcgtcccacg
     4201 cgcgcgccgc gtcgatgctc gccgcagtta tcgccaccac ggcgaaggcg acggcgcgct
     4261 gctcgccgcc ctgcgcggcc cgcccggcgt gggccgacgc ctgatcgagg agttcattag
     4321 cccgttcagg cttgtttctt ttggcggcca gttcggcggc ctgagccatg ccgaaggcga
     4381 gcgcgagcgg cggggcgtcg gcgccgcgga cgaagctcgc ggccagctcg aaatcctgct
     4441 cctcaccctc cgcgtaagcc cctcgcaggc tcaggagctg gtagaccgtg acgagcagta
     4501 gcgccttgcg ccgcgcctcc ggctgcgtga tggagtcggc gaagctctgc gccctttccc
     4561 acatcttaag ctcggcggcc ctgtgtacgg cctgaaggcg cagacggtcg cgcgccggcc
     4621 cctcatccgc tttggagatg gcgtcgagcg tcggccccag ccggtcggtc gaattgttcg
     4681 acgtgacctt ccgcacatcg gcctgcgccg agagatgtcc gcgtcgctcg gcatcgatgt
     4741 cggcggcgag cgactgaagg cgcgcgtgca actgaggggc gtggcgcggg gcctcgcgct
     4801 cgaagaaggg cagcagtcgc cgcgtcgtga aaaacagtcc gaggagtttg gattcagcgc
     4861 tcacttcatc cgcgtctggg gacggggcgg cggagcgcag gagcacgccg gccgctgtgt
     4921 cgaagaaggc gacgaggacg cgcggcgacg gcggcggttc ggccgcggga ttcttgaaag
     4981 acaggttgcg aaaattaaca gtgccgtgtg agtcgattgt cgtcaacagg ttcggggtca
     5041 gaatgtagga ggagaggaga agaacgtcac ccgagtccgc cgacgcgtcg ccgcgcatcc
     5101 tttggagcag gcggaggaag agatcgtcgg cttccgtggg ggcgtgtgcg cgcagttcga
     5161 gcaggaagtg gacgaggtcg atgctcggcc cttcggcgac gaccggcgcg gcgagcgcgg
     5221 cggccctttg cgggtcgccc tcggcaagca gcctttcggc gagttgaagc cgttgccacc
     5281 cttcgacact gacgccctcg gatggccccg cggcataaga ggtctgggcc gaccgccgcg
     5341 ggtcgctgcc gagttcgctc tgactcttca tcaactcgcc gaagaaagtt tcgcccagac
     5401 gagggtcgcg ccgcgtcgcc gctgagatga ccacgtcgcg cgcgtcggtg aaaagatgcg
     5461 gcgagttgcc gtcttcgccc gactcggcgg ccatctcctt catctcggcg aggtcggctt
     5521 cggtggccgc ctcccacgcg cgtcggaaga gagcgcgggc ggcgcgctcg tcggcgagcc
     5581 agagggcgtc ggccgcgagc gcctgaactt tcgcggagta gaaacggtcg tcgaagacgc
     5641 gcgcggcgtc ggccgcttcg aggagcgcgg acacggcccg ctgtttttcg cgtgcggcgg
     5701 aagccctcgc ggcggcgctc tgcccgccac gcttgcgcgt gttctgacct gaagcgcgcg
     5761 cgccttctcc cgtcgtctgc tgcggcggga cggaggcggc cggcgcgcag acgccttcat
     5821 gtcgccgcgt ggaagcgacg caaggcgcga cgacgacaca cgcggcaaac agtgcaactg
     5881 ggcagcgcag cttgtttgac ttgtcgggca ccacggacac ctcggctgac gaaaattatt
     5941 ccgactcgaa gagggcgccg gggcgcggcg gcgcggccgt gggggggagt ccgaagtgct
     6001 cgtaggcgag gcgcgtggcg acgcgcccgc ggggcgtgcg gttgaggaag ccgatctgga
     6061 tgaggtacgg ctcgatcagc tcctcgattg actccttctc ctcgtggagc atggccgaca
     6121 gtgtggagag tccgacgggg ccgccgtggt gctggtcgat gatcgagagc aggagcttgc
     6181 ggtcaacctc gtcgaggccg taggtgtcca cctccatgcg gttgagggcg tcgccggcca
     6241 ccaccttcgt gatgcgtccg tcgtgctcga cctcggcgaa gtcgcgcacg cgacggagca
     6301 gacggttggc gatacgcggg gtgccgcgcg cgcgtcgggc gatctcgcgc gcgccgtcgt
     6361 cgtcgatttc cgtgccgagg atttcggccg agcgccggac gatgagttcg agttcgtcgt
     6421 gcgcgtagac ggtgagatgg aagacgatgc cgaagcgtcc gcgcaaaggc gccgtgatga
     6481 gaccggcgcg ggtcgtcgcg ccgactaaag tgaacttcgg caggtctaac ttgatggagc
     6541 gggcggccgt gccctgtccg atcatcaggt cgagctgata gtcctccatc gccggataga
     6601 gcacttcttc gatggccggg tggaggcgat gaatctcgtc gatgaaaaga acgtcgccct
     6661 cttgcaggtt cgtgagcatg gcggcgaggt cgccgggctt ttcgatgacg gggccggcgg
     6721 tcgaacgcag ctcggcgccc atctcggtgg cgatgatgtt gccgagcgtg gtcttcccca
     6781 aaccgggcgg gccggacaaa aggacgtggt cgagcgactc gcgacgcttg cgggcggcgc
     6841 tgatgaagac gcgcaaattc tccgtcacct tcttctgccc gatgtactcg gccagatgcg
     6901 ccggccgaag cgacagctca aactgccgct cctcgtccgg cgctccctgc tcgcgcacgc
     6961 tgacaggctc aagctcgtcc ataaaacaga ggtcagaggt tagaggtcag aggttagtgg
     7021 aagacagttt tttactgacc tctgacctct gacctcttcg acttcatcct ttcgacaggt
     7081 gtcgcaggct gcgccgcaga agcagctcga cggagaggcc gccgccttcg
//
LOCUS       DASVES010000290        21855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_11240, whole genome shotgun sequence.
ACCESSION   DASVES010000290 DASVES010000000
VERSION     DASVES010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21855
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11240"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VF240_11665"
     CDS             <1..1332
                     /locus_tag="VF240_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX7175907.1"
                     /translation="DAENRSIWRRFSRNLSVGLLGAVLSLAIKSAQTLLLTRALRMED
                     YGRVLIVVNLFVFLNSFIGLRVSDVMFRFFPLLRERREESALQGLLLLCLGVSATTGL
                     LIFVSVLVFSPWLAGRLYQSPELAPLFGIYGWTILASSFSDVSGPILRMYDRFTSLVA
                     PQVLGSLATFTILIAYMATTSRYHLTPIVAAFAVGVFVQTVPPLVLALRLVKPYLTGV
                     NLKLAATSLATYRSELMRCLFNSNVTGYLKIANSPGDLFLLGIFSSPAQVALYGLAKQ
                     LVAPLALLLTNVQTALTPEITLLVAKRKLEQFKRLVGLYTVSAFVAGGLLTVCALLAG
                     RFLILRLTRPEYVDALPLFYILLIAAWALLVGAFLRPLALSLDLLKWDNLAQLASTAV
                     LLIFVIAGRLNALTLALVQLAGVLALRLLFYVPVWRRLRALTHDSQQKGVP"
     gene            1498..2484
                     /locus_tag="VF240_11670"
     CDS             1498..2484
                     /locus_tag="VF240_11670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175908.1"
                     /translation="MDGEEAAEEIRGVVRSGDQRREVRAPLVSAILAVRNREGSVARA
                     IESVLTQTYRPLELIVVDDGSTDGTRGVVESFGAALTLVTQAHAGVYAARNLGLRRAR
                     GELVAFIDSDDAWLPDKLAAQVPLMRPPEVGLVFGDALHVTAPHDNAPRTGLTSFRVA
                     PPRRGRAAESLAWCNFVPTCTALVRRSCLEETGGFSEASELSADYLAWFRIAMRHRLD
                     YVDRPVAEYTVHAGGISFDLGRAVAARISLFSAELARTTDGAARAVLQRLLFNLSLHL
                     ALAVPRGRARSVSDPLRLAWRTASSVASLEAVPWAAAFAANQLRRRARRLFP"
     gene            2481..3425
                     /locus_tag="VF240_11675"
     CDS             2481..3425
                     /locus_tag="VF240_11675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175909.1"
                     /translation="MTRPLVSVVIETITTRHDCPAGSLADGLTGTLDGLDRQTYPQGL
                     IERIVVLDREVTEADAGELRRRYPSVKFVSSPASNYFAAKNAGAAAAAGDIVALIDGD
                     CEPAADWLERLLAPFDPQVVAVGGRTRYTGESLAARTFSVPDFATVLADEEGLASGIL
                     LNNVAFRREVFLAHPLDPRIRRNGGCFLLFHQLRAAGARILYEPRAVVAHGPDYGGLG
                     FLRKHFDRGYDGVTVYRLDDDGVLRGTRLFRRLGAPALVAIVGRRIVFDWLRMLRHRR
                     QIGIPFVALPYFGAVALGTRLIELAGGLTATVASRSAR"
     gene            3512..4615
                     /locus_tag="VF240_11680"
     CDS             3512..4615
                     /locus_tag="VF240_11680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7175910.1"
                     /translation="MALAEGGNRVLFVENTGVRRTSLHDASRLWTRFRNWWEARGKPK
                     RVVSGIDVFSPLLLPFPYSRAAVSMNTRVLVRAVRAWLGDDGGPLIVITFLPTPLARK
                     VIGALDPALVVYYCIDRLSESSPGARRVVHSERRLLAEADLVLVTSRDLYEMTAEVTS
                     RVELLVSGVHVEQFERARRSRAEAHKKFPALPGPIVGFVGSLRNSIDLALLARVAELA
                     PDLQFVLVGPRFTDVTPLAALPNVRLLDAISHEDVMHYMVRFDVGVAPYLINQFTAAI
                     MPVKLKEYLAAGLPVVATPLPEVRRFAEQHPGLVRLAGDPAEFVASLREALSDSAPEM
                     SARRIAVARQYDWSNQMARMNESIEQALLKRLR"
     gene            complement(4693..5496)
                     /locus_tag="VF240_11685"
     CDS             complement(4693..5496)
                     /locus_tag="VF240_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175911.1"
                     /translation="MKAVLAGVVGLVLGGVLTYFLLVGAPRMEKPPGQPVRAPDAGAA
                     PAGTAVLSLDENFFNTLLGTLFAEVGTPSFNLSAGNTSAPSSWWVAPEPAGGGFRYVE
                     AQAGGCPGQVVIVGERGGTRTGVRLQAGEITSPLVFNGSYAVPLLGTCVNFSGTADAQ
                     IATFFRAEEQTLYGQINVRTVILDNVAAAYGPAITSLVQTALNRSVNPITIMRGSPLT
                     ISMPISAAGGTLRGEARDVRSEVQDGTLRLHVTYDFKGTKGQPPPQPSS"
     gene            complement(5517..6437)
                     /locus_tag="VF240_11690"
     CDS             complement(5517..6437)
                     /locus_tag="VF240_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175912.1"
                     /translation="MRLSKTIFGSTALALALSGAACGGAANTNVAVSTNATAPNVNTA
                     PSTNAAPASPADTGAATREPERYRATYVFSGQTTGATTAAAATTIEVARDGANRRWAF
                     DTKTPMLGKVVILDRTDKRYLILEGQKRYVELTPQMTGFNVPRTMTPGMMVEQLQRQP
                     NVERVGEEQVANRTAVKYRVAGQAATGTPAGQVAGETFFWIDKETGLPLKMQAATAAS
                     GQVQGATGAIGGMEVRELTTTVDAATFELPTGFTPMTEQEVQQLQQTGGMILQAIIGM
                     MGGAGGAPAGAPAPMPTTPPMMASPSPTVR"
     gene            complement(6750..8732)
                     /locus_tag="VF240_11695"
     CDS             complement(6750..8732)
                     /locus_tag="VF240_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7175913.1"
                     /translation="MKIGKQHYSLFTSLLLALALCLGGASGFASAAQTAGSGRGSGDE
                     NENTDGARDGKVSERLRGRAATGSGARLEVIIQLNGMMSGELNALLNRNGARVRKELR
                     SLGMAAVELPASAVAELENFDEVSYVTPDDDTQVLGHVSRTTGADAAAAQTYSSVSGL
                     DGTGVGIAVVDSGIYDSHSSFLDAAGASRVVVNQNFVATESRNDDPYGHGTHVAALAA
                     GGDRVQTGAYRGVAPDAKLIDLRVLGADGRGKTSYLLAALDWIAANHRTHNVRVVNLS
                     LGSPAVESYRNDPVCRAVERLYDLDIVVVAAAGNSGKNLLGQKVYGQIHSPGNSPHAI
                     TVGASNTIGTDARSDDTVATYSSRGPTRSFSTDASGARRYDNVLKPDLVAPGNKLVSA
                     EAYPNYLRTTNTSLAGYDGAGQQDDMMYMSGTSMAAPLVAGAAALMFEVNPKLTSGMV
                     RTLLQYTATPIKGYNLLEQGAGQLNVAGASKLADLVRRDLAATAAVGTAMLSAAAPAP
                     QSTIAGVNYTWAQGITFNYTSGSGVELITKLQEGYRPTYFIQDDVNPSGNGTLVKDNG
                     RLTAGVLLGRNVMTSSGGTLGTGTAIPVSLRGNGVLLSDGVLLSDGALLSDGVLLSDG
                     VLLSDGVLLSDGVLLSDVTLGTSVIVYGDLTGYMAP"
     gene            complement(9041..9814)
                     /locus_tag="VF240_11700"
     CDS             complement(9041..9814)
                     /locus_tag="VF240_11700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7175914.1"
                     /translation="MVDIERLASRYDTDKAEHTEYLRNYAQYFEPLRGRDIRLLELGV
                     YHGGSLLLWRDYFERGRVVGLDIEPVKIEDTTGRIRVYQGRQEDTALLDRVARETAPE
                     GFDIVIDDCSHIGEPTRASFWHLFDNHLKPGGLYVVEDWGTGYWDDWTDGVGYGRERS
                     RARPALYRLTRALARLQKNRTAARVPLAAGLLRRAKAATLRPQFHSHDFGMVGFVKEL
                     IDELGAADITNPEHGRGPRRRSKFREMRLSPSHLFVVKS"
     gene            complement(9839..10459)
                     /locus_tag="VF240_11705"
     CDS             complement(9839..10459)
                     /locus_tag="VF240_11705"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX7175915.1"
                     /translation="MEYLASQGQDEHLARLHTHAGWIEVIAGSMFSGKSEELIRRLRR
                     AKIARQRVQVFKPEIDERYSRDHIVSHSEMRHESRAVRTAAEIRVLVEPATEVVGIDE
                     GQFFDSDLVAVVNELADRGVRVIVAGLDQDYTGCPFGPMPHLLAVAEYITKTHAICVR
                     CGQPANYSQRTVEVEGQVVVGASDAYEARCRRCFVPHADAPTSHIE"
     gene            complement(10480..11055)
                     /locus_tag="VF240_11710"
     CDS             complement(10480..11055)
                     /locus_tag="VF240_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HEX7175916.1"
                     /translation="MIVALGSTRAAKIMAVRAACARAAGVDAAWRGAEVLARAVESGV
                     PAMPLSDTHLMRGARERAEAVRALLWETGRPADLYVGLEGGFHSITCEGRRHTFLRGW
                     AYATDGTRGFFGAAPSIEVPAEIVRRVEGQNRELGEVIDEFSGEHDVRSRQGAWGILS
                     RDLLTRSQSFESALVAAFAPFYNARVYRQTP"
     gene            complement(11239..12690)
                     /locus_tag="VF240_11715"
     CDS             complement(11239..12690)
                     /locus_tag="VF240_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7175917.1"
                     /translation="MDEPEKPAATDASPERTPDGGRVGRAPGLLRQAVFGSEQDFTKG
                     PLGRAIFLLAVPMVLEMLMESLFGVVNVFWVASLGAEAVAAVGLTESMLTIVFTVALG
                     LSMATTATVARRIGEKNPEAASRAAAQSILLGSAVAVCIGVIGIIFAPQLLQLMGAEE
                     SVVAVGANYPRIIYGSNIVIMLLFLNNAVFRGAGDASIAMRALWIANAVNLVLDPCFI
                     FGWGPFPELGVTGSAVATTIGRGAGVVYQFWMMTSGRGRVTVSFRSLTPDFPLMASLL
                     RLSVGGMLQFLVATASWVALIRIVAYFGSAALAGYTVAVRIFVFAILPSWGMSNAAAT
                     LVGQSLGAGKPERAERAVWKTAFANMLFLLGVSLVFIIFAEQLVGIFTTDPAVVPYGV
                     DCLRFISYGYVFYAYGMVIVQSFNGAGDTFTPTVINIICLWLVQLPLAYYLSMRAGMG
                     ARGVFFAITIAESLLAVVAVLVFRRGKWKERKV"
     gene            12980..14047
                     /locus_tag="VF240_11720"
     CDS             12980..14047
                     /locus_tag="VF240_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175918.1"
                     /translation="MPKTTTIIAVAVFSVAVGTLCVYFYWWAASFYKRKTAEPPPAPL
                     APRKTLWRDPGRVEKLDFAGGRGGREGFPAPPFRFVEEHIAGSSPCMSVRDARNRVWR
                     IKWGDEVQSETFASRVVWAAGYHVETNYYLAEGEIEGAKDLTRARDCVGEDCRFRPAR
                     FELDEPGVVKMFDEHGWAWDDNPFVGTRELAGLKIVFMLTSNWDNKDVRDVARGSNTA
                     IFQHRLPDGRQEARYLIIDWGATMGRWAPVGFREKWDSEAYEAQTPEFITGVTEDGIV
                     EWGYLGQRTEDATEGIRVEDVRWLHGYVGRITDAQIRTGLRASGATDDAEVERFTSAL
                     RERINQLGRVAELGAAYRPPG"
     gene            14236..15165
                     /locus_tag="VF240_11725"
     CDS             14236..15165
                     /locus_tag="VF240_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX7175919.1"
                     /translation="MTAIHVPDELRRNVTTCHGRAGVQWLDALPAILDACAARWSLRL
                     GLPFTPLSYNYVMAAERADRTPAVLKVGVPCRELETEAAALRVYDGRGCARLLEADTQ
                     LGALLIERLEPGTPLSAGLVTETDDEEATVTAASVMRELWRPAPDAHEFPRVSDWGAG
                     FNRLRARFGGGSGPFPPALVREAESLFAELLETAAAPVLLHGDLHHYNILAAGRDRWL
                     AIDPKGVVGEPAFEVTALMHNPAGLLEMRRPVEVLGRRVELLARELNLERARVRGWGI
                     AGLVLSAWWSFEDHGEGWERGVECARLLAAVKE"
     gene            15231..16049
                     /gene="speB"
                     /locus_tag="VF240_11730"
     CDS             15231..16049
                     /gene="speB"
                     /locus_tag="VF240_11730"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX7175920.1"
                     /translation="MTDHTDAAPPRLALVGFRFDENSSFLRGAADAPPLIRQALVSDH
                     SNSWAENGTDLAGAFYDAGDVTSAPDADMLSAIESSIASLLARGLSPVSLGGDHSITY
                     PIVRAIARRHPNLSILHFDAHPDLYDELLGNRHSHASPFARIMEEGLARRLVQVGVRT
                     LNGHQREQAARFGVEILEMRNWRDGVRLDFESPVYISFDVDGLDPSCAPGVSHREPGG
                     LTTRQALDVIHSVRARIVGADIVEYNPVADFQNITATVCAKLLKEIAAQMLEKQ"
     gene            complement(16112..16630)
                     /locus_tag="VF240_11735"
     CDS             complement(16112..16630)
                     /locus_tag="VF240_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HEX7175921.1"
                     /translation="MWLIQWAKSRSTHNEFGVPIPNFGKVTDALYRGALPDAAGYRAL
                     ADKLKVRRVCSIIEHESAEDARTALANGIAEWHHIAFSDRHVPEAESVRRWLDYVRSS
                     ETNGVVFTHCRGGRHRTGVLIGVLRVTDQGWTKEQAYQEMRRYGWYAALGHQPLLDWF
                     FNDFNPKDFEKQ"
     gene            complement(16786..17478)
                     /locus_tag="VF240_11740"
     CDS             complement(16786..17478)
                     /locus_tag="VF240_11740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7175922.1"
                     /translation="MAGEMVEFGSNGATARGYLSVSEAGRGPGVVVLQEWWGLVPHIK
                     DVCDRFAREGFVALAPDLYHGDVARSPDEAGKMMMALNIERTEKDLRGAIDYLLGHEA
                     VAGAVVGAVGFCMGGVLSLYAASVNEQVGACVVFYGIHPKVEPPLEHLRAPVLGLYAE
                     RDASVPPAAVRELEARLRELGKSVEMHIYPDTDHGFFNDTRPEVYDDAAAEDAWRRTV
                     AFFREHLAAGEE"
     gene            17925..18704
                     /locus_tag="VF240_11745"
     CDS             17925..18704
                     /locus_tag="VF240_11745"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7175923.1"
                     /translation="MFRPGKNPKDQPHTDDTADHTPHAAGSSSSSPASSYGSGSSASA
                     GSSSSPTSNTGAAGSDYSAASPNASRAVTESESLAREIKDGTLTGFVGNGTSLTGEAS
                     FKGMLRVDGHLSGRVSSSDGTLIVSTNGQVDANVEVAVAQIYGTVNGDIIASKRIEMG
                     RVAKVTGNVQTPALVIENGALFEGSCRMMQQREELDRPASRPETSTAASAASTSNSSS
                     TSGSSSTSPYASAAKNDKSDKAPGSTSSSGSGVTTATDAVS"
     gene            19008..20876
                     /locus_tag="VF240_11750"
     CDS             19008..20876
                     /locus_tag="VF240_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX7175924.1"
                     /translation="MSAIIDQPPAPPQERQHEEVETVTIRFAGDSGDGMQLTGTQFTN
                     TSAVLGNDISTLPDFPAEIRAPAGSLPGVSGFQLNFSSRDIRTPGDQPNVLVAMNPAA
                     LKVNLSDLEDGGTVIINTDEFNKDNLKKANYATNPLEDDTLRGYRVHRLPITTVNLRA
                     LQEAGVKLSRKEMDRCKNFFALGVLYWLYDRPMEQTLEWIKAKFSKLPEVARANEVAL
                     RVGYNFADTTEVFTTHYTVKKAAIAPGKYRKITGNEATAIGFVAASELSGRPLFYGSY
                     PITPASDILHELARHKNFGVKTFQAEDEIAAVCAAIGASFTGHIGLTGTSGPGVALKQ
                     EAIGLAVMTELPLVIINVQRGGPSTGLPTKTEQADLFQAVWGRNGECPAIVIAPATPA
                     DCFTMAIEAVRLAFKYMSPVFYLSDGYLANGAEPWLIPDIDALPKIDVRFADDPASFM
                     PYERDDRTLSRPWAIPGTPGLEHRVGGIEKEHLTGNVNYDPENHHFMVKLRQAKVDRA
                     VQDVPDVEVYGERTGKVLVLGWGSTYGSITSAVERLREEGKPVSAAHLRHLNPFPANL
                     GEVLGNFETVIIPEMNLGQLCTMIRARYLVDAQPFSKVKGRPFQIREIISKVEEYL"
     gene            20898..21461
                     /locus_tag="VF240_11755"
     CDS             20898..21461
                     /locus_tag="VF240_11755"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7175925.1"
                     /translation="MSTTTQLTTAEELLKLPRGRFRYELVNGELRQMSPAGQNHGKIA
                     ARLTVALGQHVEEKNLGEVYAAETGFKLRTSPDTVRAPDASFVARTRADEVGESKGYF
                     PGPPDLAVEVVSPDDTVREVEEKVAEWLEYGTRLVWVVSPKLHAVTVYRSLTDIETLT
                     ENDALDGETVVPGFSYPVAKLFAGRSS"
     gene            21538..>21855
                     /locus_tag="VF240_11760"
     CDS             21538..>21855
                     /locus_tag="VF240_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175926.1"
                     /translation="MSTSGNGSNGNKKVDKSAAGIGGNVSGGGPSPESEPGSVVAGAP
                     APPPGKLTKADFSSDQEVRWCPGCGDYAILNNIQKVMPELGIPREKIVFVSGIGCSSR
                     FPYY"
BASE COUNT         3574 a         7258 c         7302 g         3721 t
ORIGIN      
        1 gacgctgaga atcgctctat ctggcgccgc ttcagtcgca acctttcggt cgggcttttg
       61 ggggcagtgc tctccctggc cattaaatca gctcaaaccc tgctcctgac gagggcgctc
      121 cggatggagg attatgggcg cgtgctcatc gtcgtcaacc tcttcgtctt tctgaactct
      181 tttatcgggc tgcgcgtgag cgacgtgatg ttccgcttct ttccgctgct ccgagagcga
      241 cgggaagaaa gcgcgcttca gggacttctt ctcctttgtc tgggggttag cgccaccacc
      301 ggcctgctga ttttcgtcag cgtcctcgtc ttctccccct ggttggccgg acgcctttac
      361 caaagccccg agctggcccc gcttttcggc atctacggct ggacgattct ggcttcatcg
      421 ttcagcgacg tgtccgggcc gattctgcgg atgtatgacc gcttcacgtc cctcgtggcg
      481 ccgcaggttc tgggcagtct ggccacattc acaattctta tcgcgtacat ggcgacgacg
      541 agccgctatc acctgacgcc catcgtcgcg gccttcgcgg tcggcgtctt tgtccagact
      601 gtgccgccgc tggtgctggc tctgcgactg gtcaagccat acctcacggg cgttaatcta
      661 aaacttgccg ccacgtcctt agccacatac cgctctgagc tgatgaggtg tctgttcaat
      721 agtaacgtca cgggatacct taaaattgcg aactcccccg gcgacctctt cctgctgggc
      781 atcttctcct cgcccgcaca ggtcgccttg tacggcctcg ccaagcaact ggtggcgccc
      841 ctggcgcttt tactgaccaa tgttcagacc gcgctcaccc cggagataac tttgctcgtg
      901 gccaagcgga agttggagca attcaaacgt cttgtcggcc tctacaccgt gtcggcattc
      961 gtcgcgggag gtctgttgac ggtctgcgcc ctcctcgcgg ggcgcttttt gatcttgcgg
     1021 ctcacccggc cggagtatgt tgacgccctg ccgttgttct atattttatt aatcgccgcg
     1081 tgggcgctcc tcgtcggcgc atttttaaga ccgctcgctc tgagtctgga tttgctgaag
     1141 tgggataatc tggcgcaact ggcgagcacc gccgtcctgc tcatcttcgt tatcgccggc
     1201 cgactcaacg ctttgacctt ggctcttgtt caactggcgg gcgtgctggc gctgcgtctc
     1261 ctgttttacg tgccggtgtg gaggcgactt cgcgcgctga cccatgattc acagcagaag
     1321 ggtgtgccct agagttcacc tccgcgccgg gccgctgttc tgaaaggact ggtgcggaaa
     1381 cacgtcggca aagtttcccc ctcccttttc ctttagaggt cgcgggtgtt gagtggacga
     1441 tggtggatgg cggctgtatc ttttgatctc taaggcgtgc gtgcgtggag taagcggatg
     1501 gatggagaag aggcggcgga ggaaattcga ggcgtcgtga gatcaggcga tcaacggcgc
     1561 gaggtgcgcg ccccgctcgt ctccgcaatc ctcgcggtcc gtaatcggga ggggtcggtc
     1621 gcgcgcgcca tcgagagcgt cctgacgcag acctaccgcc cactggagct gatcgtcgtt
     1681 gatgacggct ccacggacgg tacgcgcggc gtggtggaga gtttcggcgc ggcgctcacg
     1741 ctcgtcacac aggcgcacgc cggagtctac gcggcgcgca acctcggcct gcgccgcgcg
     1801 cgcggcgagc tggtcgcctt catcgactcg gacgacgcgt ggcttcccga caaactcgcc
     1861 gcgcaggtgc cgctgatgcg cccgcccgag gtggggctgg tcttcggcga cgccctccac
     1921 gtcacggcgc cgcatgataa cgcgccgcgc accgggctga cgtcgttccg cgtggcgccg
     1981 ccgcggcgcg ggcgagccgc cgaaagtctc gcgtggtgca acttcgtccc gacctgcacc
     2041 gcactcgtgc gccgctcctg cctcgaggag acgggcggct tctccgaggc gagcgagctt
     2101 tccgcagact atctcgcgtg gttccgtatc gctatgcgcc acaggctgga ttacgttgac
     2161 cgccccgtgg ccgaatacac cgtacacgcc gggggcatca gcttcgacct cggccgcgcg
     2221 gtcgccgcgc gcatctcact cttctccgct gaacttgcgc ggaccactga cggggcggcg
     2281 cgcgccgtgc tccagcggct gctcttcaac ctctcactgc atctcgccct cgccgtgccg
     2341 cgcgggcgcg cgcgcagcgt ctccgacccg ctgcgactcg cctggcgcac ggcgtcgagc
     2401 gtggcgagcc tcgaagcagt gccctgggcc gccgcgttcg cggccaatca actgcgccgg
     2461 agggcgcgac gactcttccc atgaccagac ctctcgtcag cgttgtcatc gaaacgatca
     2521 cgacgcgcca cgactgcccg gccggctctc tggccgacgg cctcaccggc acgctcgacg
     2581 gtctcgaccg gcagacgtac ccgcaagggc tgatcgagcg tatcgtcgtc ctcgaccgtg
     2641 aagtgaccga ggccgacgcc ggcgagcttc gccggcgcta cccgtcggtg aaattcgtgt
     2701 cgtcgccggc gagcaactac ttcgccgcga agaacgccgg cgcggcggcg gcggcgggcg
     2761 acatcgttgc gctgatcgac ggcgactgtg agcccgcggc cgactggctg gagaggctgt
     2821 tggcgccgtt cgatcctcag gtcgtcgctg tcggcgggcg cacccgttac accggcgagt
     2881 cgttggccgc gcggacgttc tccgtccccg acttcgccac cgtcctggcc gatgaggagg
     2941 gactggcgtc cggtatcctc ctcaacaacg tggccttccg gcgcgaggtg tttctggcgc
     3001 atccgctgga cccacgcatc cgccgcaacg gaggctgctt ccttctcttt caccaactcc
     3061 gcgcggccgg cgcgcgcatc ctctacgaac cgcgcgcggt ggtcgcgcac ggtcccgact
     3121 acggcggtct cggctttctg cgcaagcact tcgaccgcgg gtacgacggc gtcacggtct
     3181 accgactgga cgacgacggc gtgcttcgcg ggacgcgcct cttccgccga ctcggcgccc
     3241 ccgcgctggt ggcgattgtc ggacgccgca tcgtcttcga ctggttgcgc atgctgcggc
     3301 accgtcgcca gatcggcatc ccgttcgttg ccctgccata tttcggggcc gtcgcgctcg
     3361 ggacgcgcct catcgagctg gcgggtggtc tgacggccac cgtcgcatcg agatcggcga
     3421 ggtaggagag ccgatgcccc gaggtgcctc catcgtttgc ctgtcttcta tcgactggtc
     3481 attcaacaga cagaatccgc aggaagtcgc cgtggccctg gccgaaggcg gcaaccgagt
     3541 tctctttgtc gagaacacgg gtgtccggcg cacgtccctg cacgacgcgt cgcggctctg
     3601 gacgcgtttt cggaactggt gggaggcccg cggcaaaccg aagcgcgtgg tgagcggcat
     3661 cgacgttttc tcgccgctgt tgctgccgtt cccgtactcg cgggccgcgg tttccatgaa
     3721 cacgcgtgtg ctcgtccgcg ccgtccgcgc atggctggga gatgacggcg gtccgctgat
     3781 cgtgatcacc tttctgccga cgccgcttgc gcgcaaggtg atcggcgcgc tcgatccggc
     3841 gctcgtcgtc tactactgca tcgaccgtct gtcggaaagc tcgccgggcg cgcgcagggt
     3901 ggtgcattcg gagcgccggc tcctcgccga ggctgatctg gtgctggtga cctcgcgcga
     3961 cctctacgag atgacggcgg aagtgacctc gcgcgtagag ttgctggtga gcggcgtcca
     4021 cgtcgagcaa ttcgagcgcg cgcggcggtc gcgcgcggag gcgcataaga aatttccggc
     4081 tctgccgggg ccaatcgtcg gcttcgtagg cagccttcgc aactcgattg acctcgcgct
     4141 gctggcgcgc gttgcggaac tcgcaccgga tttgcagttt gtgctggtag gcccccgctt
     4201 caccgacgtc acgccgctgg cggccctgcc caacgtgcgg ctgctcgacg ccatctcgca
     4261 cgaagacgtg atgcattaca tggtgcggtt cgatgtcggc gtcgcgccgt atttgatcaa
     4321 tcaattcacc gccgccatca tgccggtgaa gctcaaggaa tatttggcgg cgggtctgcc
     4381 ggtggtggcg acgccgctgc cggaagtgcg ccgttttgcg gagcagcatc ccggtctcgt
     4441 caggctggcc ggcgacccgg cggaattcgt cgcctccctg cgcgaggcgc tctccgacag
     4501 cgcgcccgag atgtcggcgc gccgcatagc cgttgcgcgc caatacgact ggagcaatca
     4561 gatggcgcgg atgaatgagt cgattgagca ggcgctgctt aagcgtctgc ggtgagtcct
     4621 cgaaagcaag acgggcggcg tggttgcgcc gcccgtcgag tttcactttt gccttcttta
     4681 gtttgttttg ccttacgagc tgggctgagg cggcggctgc cccttcgtgc ccttgaagtc
     4741 gtaggtgacg tggaggcgga gcgtgccgtc ctgaacttcg gagcgcacgt cgcgggcctc
     4801 gccgcggagc gtgccgccgg cggcgctgat gggcatcgag atcgtgagcg gcgagccgcg
     4861 catgatggtg atcgggttga cggagcggtt gagcgccgtc tggacgagcg aggtgatggc
     4921 ggggccgtag gccgccgcga cgttgtcgag gatgacggtg cggacgttga tctgcccgta
     4981 gagcgtctgc tcctcggcac ggaagaaggt ggcgatctgc gcgtcggccg tgccgctgaa
     5041 gttgacgcag gtgccgagga gggggacggc gtaactgccg ttgaagacga gcggcgaggt
     5101 gatctcgccc gcttgcagac gcacgcccgt gcgcgtcccg ccgcgctcgc cgacgatcac
     5161 cacctggccg ggacagcccc ccgcctgcgc ctcgacgtag cggaagccgc cacccgccgg
     5221 ctcaggagcg acccaccatg acgacggcgc actggtgttt cccgccgaca ggttgaacga
     5281 cggcgtgccc acctcggcga agagcgtgcc gagaagcgtg ttgaagaaat tctcgtcgag
     5341 cgagagcacg gccgtgccgg caggcgcggc gcccgcgtcg ggcgcgcgca cgggctggcc
     5401 cggcggcttc tccatgcgcg gcgcgccgac gagaagaaag tatgtgagca cgccgccgag
     5461 cacgaggccg acgactccag ccagaactgc cttcatctct taaactcccc tcgatcttag
     5521 cggactgtcg gcgacggaga cgccatcatc ggtggcgtcg tcggcatcgg ggccggagcg
     5581 cccgccggtg cgccgcccgc gccgcccatc atcccgatga tcgcctgcaa gatcatgccg
     5641 ccggtctgct gcaactgctg cacctcctgc tcggtcatcg gcgtgaagcc ggtgggcagc
     5701 tcgaaggtgg cggcatcgac cgtggtcgtc agctcacgca cttccatccc gccgatggcc
     5761 cccgtcgcgc cctgcacctg acccgacgcg gcggtcgccg cctgcatctt cagcggcagg
     5821 cccgtctcct tgtcgatcca gaagaacgtc tcgccagcga cctgaccggc cggcgtgccc
     5881 gtcgccgcct gtccggcgac gcggtacttg acggccgtgc ggttcgccac ctgctcctcg
     5941 cccacgcgct cgacgttcgg ctgccgctgc aactgctcga ccatcatgcc gggcgtcatc
     6001 gtgcgcggca cgttgaagcc ggtcatctgc ggcgtcagct cgacgtagcg cttctggcct
     6061 tcgaggatga ggtaacgctt gtcggtgcgg tcgaggatga cgaccttgcc gagcatcggt
     6121 gtcttggtgt cgaaggccca gcggcggttc gcgccgtcgc gcgcgacctc gatggtggtg
     6181 gccgcggccg cggttgtcgc gcccgtcgtc tgccccgaga agacgtaggt ggcgcggtag
     6241 cgttccggct cgcgcgtggc ggcgccggtg tcggccggcg aggccggggc ggcgttcgtg
     6301 gatggcgcgg tgttgacgtt cggggcggtc gcattggtgc tcacggccac attcgtgttc
     6361 gccgcgccgc cgcaagccgc gccggagagc gcgagggcga gggccgttga gccgaagatc
     6421 gttttcgaca gacgcatgac agtgttctcc tcaatcacag gcagaagttt tccgagctta
     6481 aattttccgg gggatgaacc tgcgctgagg ataccacaaa aacgggtgcg ggcggagatg
     6541 cgcgcggcgt ccgaagaggc cgctcgaaag gcgacggacg gcaaggcgac ggcgcggcgc
     6601 gctcccgtgc ggtttgcggt tgcgctgacc gacaaatgtt tagaggcggt ctctgaaccg
     6661 ccggctcaac tgagacgcag cttgagccgg cggtcagaga ccgcctctaa acggtcggta
     6721 aatttcggta agttgacgcc ctgaccgcgc tagggggcca tgtagcccgt caagtctccg
     6781 tagacgatga cggacgtgcc cagcgtgaca tcgctcaaca ggacgccgtc ggagagcagc
     6841 acgccgtcgg agagcaacac gccgtcggag agcaggacgc cgtcgctcag gagggcgcca
     6901 tcagagagca ggacgccgtc ggagagcagg acgccgttgc cgcgcaggct gacggggatg
     6961 gccgtgcccg tgccgagcgt gccgccgctg ctggtcatca cgttcctgcc gaggaggacg
     7021 ccggccgtga gcctgccatt atccttcacc agcgtgccgt tgcctgaggg gttcacgtcg
     7081 tcctgtatga agtaggtggg acgatatccc tcctgcaact tggtgatcag ctcgacgccc
     7141 gagccggagg tgtagttgaa ggtgatgccc tgcgcccagg tgtagttgac gccggcgatg
     7201 gtggactgcg gggccggggc cgcggccgag agcatcgccg tgccgacggc ggccgtggcg
     7261 gcgaggtcgc ggcgcacgag gtcggcgagt ttggacgcgc cggcgacgtt gagctggccc
     7321 gcgccctgct ccaacaggtt gtagcccttg atgggcgtgg ccgtgtactg aagcagtgtt
     7381 ctgaccatgc cggaggtcag cttcgggttg acctcgaaca tcagcgccgc cgcgccggcg
     7441 acgagcggcg ccgccatcga cgtgccgctc atgtacatca tgtcgtcctg ctggccggcg
     7501 ccgtcgtagc cggcgagcga cgtgttcgtg gtccgcaggt agttggggta cgcctcggcc
     7561 gagaccagct tgttgccggg cgcgacgagg tcgggcttga ggacgttatc gtagcgccgg
     7621 gcgccggacg cgtccgtcga gaagctgcgg gtggggccgc gcgagctgta ggtggcgacg
     7681 gtgtcgtcgg agcgcgcgtc ggtgccgatg gtgttcgagg cgccgacggt gatggcgtgg
     7741 gggctgttgc cgggcgagtg aatctgtccg tagaccttct gcccgaggag gttctttccg
     7801 ctgttgccgg ccgcggcgac gacgacgatg tcgaggtcgt agagacgctc gacggcgcgg
     7861 cacacggggt cgtttcggta agactcgacg gccgggctgc ccagagagag attgacgacg
     7921 cgcacgttat gggtgcggtg gttggcggcg atccagtcga gcgcggcgag aagataagag
     7981 gtcttgccgc ggccgtcggc gcctagcacg cgcaggtcga tgagtttggc gtcgggggcg
     8041 acgccgcggt atgcgcccgt ctggacgcgg tcaccaccgg cggccagcgc ggcgacgtgc
     8101 gtgccgtggc cgtaggggtc gtcgttgcgc gactcggtgg cgacgaaatt ctggttgacg
     8161 acgacgcggc tcgcgccggc ggcgtcgagg aaggaagagt gagagtcgta gatgccggaa
     8221 tcgacgacgg cgatgccgac gcccgtgccg tccagcccgg agacgctgct ataggtttgc
     8281 gcggccgcgg catccgcgcc ggtcgtgcgc gagacgtggc cgagcacctg cgtgtcgtcg
     8341 tccggcgtga cgtaagacac ttcgtcgaag ttctccagct ccgcgacggc cgacgccggc
     8401 agctcgactg cggccatccc gagcgagcgc agctccttcc tgacgcgtgc gccgttgcgg
     8461 ttgagcaagg cattgagttc accgctcatc atcccgttga gttgaataat cacttcgagc
     8521 cgcgcgccgc tgccggtcgc cgcgcgcccg cgcagcctct ccgacacctt gccgtcccgt
     8581 gcgccgtcgg tgttctcgtt ttcgtcgccg ctcccgcggc ccgaccccgc agtctgtgcg
     8641 gccgaggcga agcccgacgc gccgccgagg cagagcgcga gggccagcag gagagaagta
     8701 aagagactgt aatgttgctt tccgattttc atttttctgt tcgttcgcga gcgagtcgtt
     8761 cgtcaaaagc cctgaagagg tgtcacagat atttagcgag gcgggcgttt ttttaatgac
     8821 ggcgagagca catgcggact cacgcccgcc ttcccttagc tagaggcgtg ccaaacgctt
     8881 aggcccttgt aaatgtggaa tttgtgtggc ggggcatggg ggcgcgattg ccagtctgac
     8941 cgaagccggt caacctggcg cacgcggcat atcaaactga tgaggacggt cgtgggggcg
     9001 gcgttgccgc gcaagagggc cgggtgagag ggcggccccg tcaggacttc acgacgaaga
     9061 ggtggctcgg tgacagacgc atctcgcgaa acttcgaccg ccgccggggg ccgcgcccgt
     9121 gctcggggtt ggtgatgtcg gcggcgccga gttcgtcgat cagttctttg acgaagccga
     9181 ccatgccgaa gtcgtggctg tggaactgtg gccgcaaggt cgcggccttc gcccgccgca
     9241 ggaggccggc ggcaaggggc acgcgcgcgg ccgtgcggtt cttttgtaga cgcgcgagcg
     9301 cccgcgtcag gcggtagagg gcgggccggg cacgcgagcg ttcgcgaccg tagccgacgc
     9361 cgtccgtcca gtcgtcccag tagcccgtgc cccagtcctc gacgacgtag aggccgcccg
     9421 gcttaaggtg attatcgaag aggtgccaga agctcgcgcg cgtcggctcg ccgatgtgcg
     9481 aacagtcgtc gatgacgatg tcgaagccct cgggggccgt ctcgcgggcg acgcggtcaa
     9541 gcagcgcggt gtcctcctgc cggccctgat agacgcgaat cctgcccgtc gtgtcttcga
     9601 ttttgacagg ctcgatgtcg aggccgacta cgcggccgcg ctcgaagtag tcgcgccaga
     9661 gcaggagcga gccgccgtgg tagacgccga gttcgagcag gcggatgtcg cggccacgga
     9721 gcggctcgaa gtactgagcg taatttcgca ggtactcggt gtgctccgcc ttgtcggtgt
     9781 cgtagcggct ggccaaacgt tcgatgtcaa ccattgatct tccgcgttgg cgtcggcctc
     9841 attcgatgtg gctggtgggg gcgtcggcgt gggggacgaa gcagcggcgg cagcgggcct
     9901 cgtaggcgtc cgaggcgccg acgacgacct ggccctcgac ctcgacggtg cgctgcgagt
     9961 agttggccgg ctggccgcag cggacgcaga tggcgtgtgt cttggtgatg tactcggcca
    10021 cggccagaag gtgcggcatc ggcccgaagg ggcagcccgt gtagtcctga tcaaggccgg
    10081 cgacgatgac gcgcacgccg cggtcggcca gctcgttgac gacggcgacg aggtcggagt
    10141 cgaagaactg tccctcgtcg atgccgacga cctcggtcgc cggctccacc agcacgcgta
    10201 tctcggcggc ggtgcgcacc gcgcgcgact cgtggcgcat ctcggagtgg gagacgatgt
    10261 ggtcgcgcga gtagcgctcg tcgatttcgg gcttgaagac ctgcacccgc tgacgcgcga
    10321 tcttggcccg gcgcaggcgt cggatgagtt cttcgctctt gcccgagaac atggagccgg
    10381 cgatgacctc gatccagccg gcgtgcgtgt gcagccgcgc gaggtgctcg tcctgaccct
    10441 gtgatgcgag atattccatc tggctttcgg tctctcccct tatggtgttt gccggtagac
    10501 ccgcgcgttg tagaacggcg cgaacgcggc gacgagcgcc gactcgaagc tctgcgagcg
    10561 cgtcagcagg tcgcgcgaga ggatgcccca cgcgccctgc cggctacgca cgtcgtgctc
    10621 gccagagaac tcgtcgatga cctcgcccag ctcgcggttc tgcccctcga cgcggcggac
    10681 gatctcggcg ggcacctcga ttgacggggc cgcgccgaag aagccgcgcg tgccgtcggt
    10741 ggcgtaggcc cagccgcgca ggaacgtgtg acgccggccc tcgcaggtga tcgagtggaa
    10801 gccgccttcg aggccgacgt agaggtcggc cggtctcccc gtttcccaca gcagcgcgcg
    10861 cacggcctcg gcccgctcgc gcgccccgcg catcaggtgc gtgtcgctca gcggcatcgc
    10921 cggcacgccc gactcgacgg cgcgcgccag aacttccgcg cccctccacg cggcgtccac
    10981 cccggccgcg cgcgcgcagg ccgcgcgcac cgccatgatc ttggccgcgc gtgttgagcc
    11041 gagggcgacg atcatcgtgg aagcaccttc cctagcgccg gacagcggag agctaaaggg
    11101 acagccgggc cgggcgcggg caaaagctac aacacggcgg cgcggttcgt caaagagcag
    11161 tgataagtga tgagtgatga gtgataagta ggaagacgga ttttcttatc actcatcact
    11221 catcacttat cactgccctt aaaccttccg ctccttccac ttgccgcggc ggaagacgag
    11281 gacggcgacg acggcgagga gcgattcggc gatggtgatg gcgaagaaga cgccgcgcgc
    11341 gcccatgccg gcgcgcatcg agaggtagta ggcaagcggg agttggacga gccagaggca
    11401 gatgatgttg atgacggtgg gcgtgaaggt gtcgcccgcg ccgttgaagg actgcacgat
    11461 gaccatgccg taggcgtaga agacatagcc gtaagagatg aagcgcagac agtccacgcc
    11521 gtagggcaca acggccgggt cggtcgtaaa tatcccgacg agttgttcgg cgaagataat
    11581 gaagacgagg ctcacgccga gcaaaaagag catgttggcg aacgccgtct tccacacggc
    11641 ccgctcggcg cgctcgggct tgccggcccc gaggctctgt ccgacgagcg tggccgccgc
    11701 gttcgacatc ccccacgaag gcaggatggc gaagacgaag atacgcacgg cgaccgtgta
    11761 gccggccagc gcggcgctgc cgaaataggc gacgatgcgg atgagcgcga cccaactcgc
    11821 cgtcgccacg aggaactgga gcatcccgcc cacggagaga cgcagcaggc tcgccatgag
    11881 cgggaagtca ggcgtcaggc tgcggaagct gacggtaacg cgcccgcgcc cgctcgtcat
    11941 catccagaac tggtagacga cgccggcgcc gcgcccgatg gtcgtcgcca cggctgaacc
    12001 cgtgacgccg agttcgggga acggccccca cccgaagatg aagcacgggt cgaggacgag
    12061 gttgacggcg ttggcgatcc agagggcgcg catggcgatg gaggcgtcgc ccgcgccgcg
    12121 gaagacggcg ttgttgagga acagcagcat gatgacgatg ttgctgccat agatgatgcg
    12181 cgggtagttg gccccgacgg cgaccacgct ctcttcggcc cccatcagtt gcaaaagttg
    12241 cggcgcgaag atgatgccga tgacgccgat acaaacggcc acggccgagc cgagcagaat
    12301 cgactgcgcc gccgcccgtg acgcggcctc cgggttcttc tcgccgatgc gtcgcgccac
    12361 ggtcgccgtc gtcgccatgc tcaggccgag cgcgaccgtg aagacgatgg tcagcatcga
    12421 ctccgtcagt cccaccgcgg ccaccgcctc cgcgccgagg ctcgccaccc agaagacatt
    12481 gacgacgccg aacagcgact ccatcaacat ctccagaacc atcggcacgg ccagcagaaa
    12541 gatcgcccgc cccaaaggcc ctttggtgaa gtcctgctcg gagccgaaga cggcctgacg
    12601 cagcaggccc ggcgcgcggc cgacgcggcc cccgtccggc gtgcgctcgg gcgacgcgtc
    12661 cgtcgccgca ggtttttccg gttcgtccat gagaccttct ttgaagtcgt gcgcgcggga
    12721 tcgtagacat cggacgagct gccgcgcgag agtggatgat agttcacgcc ttgatacgtc
    12781 cgaaggtgaa agaagtttag cgtccacttc atcggctcat acagtaccgc gaacggaagc
    12841 ctcgcgggtc tcgcgtgcga cgagcagccc gccccgcgca tgcgagaccc gcgaggcttc
    12901 cgctcgcggt actgtggttc tggcaaatga actttcgcgg aggcgtgatg ttatagtggg
    12961 gcatccttta atcgcgtata tgccaaagac cacgacaatc atcgcggtgg ccgtcttcag
    13021 cgtcgccgtc ggcacgctct gcgtctactt ctattggtgg gccgcctcct tctacaagcg
    13081 caagacggcc gagccgccgc ccgcgcccct cgccccgcgc aagaccctct ggcgcgaccc
    13141 cggccgtgtt gaaaaactcg acttcgccgg cgggcgcggc gggcgcgagg gcttcccagc
    13201 gccgcccttc cgcttcgtcg aggagcacat cgcggggtcg agtccgtgca tgtctgtgcg
    13261 cgacgcacgc aaccgcgtgt ggcgcatcaa gtggggcgac gaggtgcagt ccgagacctt
    13321 cgcctcgcgc gtcgtctggg ccgccggtta tcacgtcgag acgaactact acttggccga
    13381 gggtgagatc gaaggtgcga aggacttgac gcgggcgcgc gactgcgtcg gcgaagactg
    13441 tcgcttccgc cccgcgcgct tcgagctgga cgagccgggc gtcgtcaaga tgttcgacga
    13501 gcacgggtgg gcttgggacg acaacccgtt cgtggggacg cgcgagctgg cgggcctcaa
    13561 gatcgtcttc atgctcacct cgaactggga caacaaggac gtgcgcgacg tggcccgcgg
    13621 ctcgaacacg gccatcttcc agcaccgcct cccggacggc cggcaggagg cgcgctacct
    13681 gatcatcgac tggggcgcca cgatgggccg ctgggcgccc gtgggctttc gcgagaagtg
    13741 ggacagcgag gcttacgagg cgcagacgcc ggagttcatc acgggcgtca cagaggacgg
    13801 catagtcgag tggggctacc tcggacaacg gacggaggat gcgaccgaag gcatccgcgt
    13861 cgaggacgtg cgctggctcc acggttatgt cggccgcatc accgacgcgc aaatccgcac
    13921 ggggctgcgc gcctccggcg ccaccgacga cgccgaggtc gaacgcttca caagcgccct
    13981 gcgcgagcgc atcaaccaac tcggccgcgt cgcggaactc ggcgccgcct accggccgcc
    14041 cggttagagc aaacttaggt tgagtgtggc gggggctgac tcaccgcaga ggcgcggagg
    14101 ccgcagagtt cacgcagagg gttgattctg ttcaagcctc cgcgccccct ccgcgtcctc
    14161 cgcgcctccg cggtgaaccg acttacacca cactcaactc aagaacgacc cggaaccttt
    14221 gaattgttga agagcatgac ggccatccac gtgcccgacg agttgaggcg aaacgtcacc
    14281 acctgccacg ggcgtgcggg ggtgcagtgg ctcgacgcgc tcccggcaat cctcgacgcc
    14341 tgcgccgcgc gctggtcgct ccgcttgggc ctccccttca cgccgctctc ctacaactac
    14401 gtgatggccg ccgagcgcgc cgaccggacg ccggcggtgc tgaaggtcgg cgtcccctgc
    14461 cgcgagttgg agacggaggc cgcggcgctc cgcgtctacg acgggcgcgg ttgcgcgcgt
    14521 ctgcttgaag cggacacgca gctcggcgcg ctgctcatcg agcggctgga gccgggcacg
    14581 ccgctctcgg ccggactcgt cacggagacc gacgacgagg aggcgaccgt gacggccgcc
    14641 tcggtgatgc gcgaactgtg gcgtcccgcg cccgacgcgc atgagttccc gagggtgtcc
    14701 gactggggcg cgggcttcaa tagattacgc gcgcgcttcg gcggcggaag cggcccgttc
    14761 ccgcccgcgc tcgtccgtga ggcggagtcg ctgttcgccg agttgcttga gacggccgcc
    14821 gcgcccgtcc tcctgcacgg cgacctgcac cactacaaca tcctcgcggc cgggcgcgac
    14881 cgctggctgg ccatcgaccc gaagggcgtc gttggtgagc cggccttcga ggtcacggcg
    14941 ctcatgcaca acccggcggg gctgctcgaa atgcgccgcc ccgttgaagt cctcggccgg
    15001 cgcgtcgaac tgctcgcgcg cgagttgaat ctggagcgtg cgcgcgtgcg cggctggggc
    15061 atcgccgggt tggtgctctc ggcgtggtgg tcgtttgagg atcacggcga agggtgggag
    15121 cggggcgtcg agtgcgcgcg gctgctggcg gcggttaaag agtaagacgc cgaaacacct
    15181 gtcgggtcgg cgctgtgaca cggcgacggc gcgcgttcta tcatcgcaac atgacagacc
    15241 acacggacgc cgcgccaccg cgactcgccc tcgtcggctt tcgcttcgac gagaactcct
    15301 cgttcctgcg cggcgcggcc gacgccccgc cgctcatccg tcaggcgtta gtctccgacc
    15361 actcgaactc ctgggccgag aacggcacag acctcgccgg cgccttctac gacgccggcg
    15421 acgtgacgag cgcgcccgac gccgacatgc tctcggcgat tgaaagctca atcgcgtcgc
    15481 tgctcgcgcg cggcctctcc cccgtctcgc tcggcggcga ccactcgatc acgtacccca
    15541 tcgtcagggc catcgcccgt cgtcacccca acttgagcat cctccacttc gacgcgcacc
    15601 cagacctcta cgacgagctg ctgggcaacc gccactcgca cgcctcgccg tttgcgcgca
    15661 tcatggagga ggggctggcg cggcgtctcg tgcaggtcgg cgtccgcacg ttgaacggcc
    15721 accagcggga gcaggccgcg cgcttcggcg tcgagattct ggagatgagg aactggcgcg
    15781 acggcgtgag actcgatttt gaatcgcccg tctacatcag cttcgacgtg gacggtctcg
    15841 acccgtcgtg cgcgcccggc gtctcgcacc gcgagccggg cggactgacg acccggcagg
    15901 cgctcgacgt aattcacagc gtccgcgccc gcatcgtcgg cgccgacatc gtcgagtaca
    15961 acccggtggc cgactttcag aacatcacgg ccaccgtgtg cgccaagctc ctcaaggaga
    16021 ttgccgcgca gatgcttgaa aagcagtgat gagtggtaag tgataagtga taagaaaagc
    16081 agtttgccct tctcttatca cttatcactc atcactgctt ttcaaagtct ttcgggttga
    16141 aatcgttgaa gaaccagtcg aggagcggtt ggtggccgag ggcggcgtac cagccgtagc
    16201 ggcgcatctc ttggtaagcc tgctctttcg tccagccctg gtcggtgacg cgcaggacgc
    16261 cgatgaggac gcccgtgcgg tggcggccgc cgcgacagtg tgtgaagacg acgccgttgg
    16321 tttccgacga gcgcacgtag tcgagccagc ggcggacgct ctcggcttcc ggcacgtggc
    16381 ggtcggagaa ggcgatgtgg tgccactcgg cgatgccgtt ggcgagcgcc gtgcgcgcgt
    16441 cctccgcgct ctcatgttcg atgatgctgc acacgcggcg cactttgagc ttgtcggcga
    16501 gagcgcggta acccgccgcg tccgggagcg cgccgcggta gagcgcgtcg gtgaccttgc
    16561 cgaagttagg gatgggcacg ccgaactcat tatgtgtcga gcgcgacttc gcccactgaa
    16621 tcagccacat ccccacatcc cccttcgcgc ctttgcgttc atttgcctga agcacagtac
    16681 cgcgagcgga agcctcgcgg gtatgcctac aatgagaggc ccgcatcgcg cacgcgagac
    16741 ccgcgaggct tccgctcgcg gtactgtttg attgagccga cgatctcact cctcgccggc
    16801 cgcgaggtgt tcgcggaaga aggcgaccgt gcgccgccac gcgtcttcgg ccgcggcgtc
    16861 gtcgtagact tccggccgcg tgtcgttgaa aaagccgtgg tcggtgtcgg ggtagatgtg
    16921 catctcgacg gacttgccga gttccctgag ccgcgcttca agctcgcgca ccgccgcggg
    16981 cggaacggac gcgtcgcgct cggcgtagag tccgagcacg ggcgcgcgca aatgttccag
    17041 cggcggctcg accttcgggt ggatgccgta gaagacgacg cacgcgccga cctgctcgtt
    17101 caccgacgcg gcgtagagcg agagcacgcc gcccatgcaa aagcccacgg cgccgacgac
    17161 ggcgcccgcc acggcctcgt gtccgagcag gtagtctatc gcgccgcgca ggtctttctc
    17221 cgtccgctcg atgttgaggg ccatcatcat cttgccggcc tcgtcgggcg agcgcgccac
    17281 gtcgccgtgg tagaggtcgg gcgcgagcgc cacgaagccc tcgcgcgcga agcggtcgca
    17341 cacatccttg atgtgcggga cgagtcccca ccactcctgc aagacgacga cgcccggccc
    17401 acggcccgcc tcggagacag acagatagcc ccgcgccgtc gcgccgttgc tcccaaactc
    17461 gaccatctcg cctgccatcc cccgaacccc cttcaactga tgacgtaaac gctgaccgcg
    17521 tgtgtgtgtg tgatacggcg cgggcacagt agtctcgtcg cccgccggtt gtcaattcct
    17581 ccccctcggc cgggggcgtg aaacgctgcc ccgattcgtc gttacaagag gcgtcggggg
    17641 aatttaacta gacacgccgc gcccgcgggg ctatatttgc ggcgcttgcc gaagggtttt
    17701 tgtcgaaggc gcggccgcac ctgaaggggc cggccgcgcc aagccactcc aaaccggcct
    17761 tacatccgca agacggagcg ggcgccgcac gcgcggcgcg tatttgtctc gactccgaac
    17821 gcgcggcgcg ccgcccacga agcgggcagc caccgccgcc gccgccggcc cccgaagtgg
    17881 gggcgagacc ccacacacga aaagttttca gggagaagac ggaaatgttc agaccgggaa
    17941 agaatccgaa agaccagccg cacaccgacg acacggctga ccacacgccg cacgccgccg
    18001 gcagcagcag cagcagcccc gcctcctctt acggctcggg gtcttccgcg tcagccggct
    18061 catcttcatc gcccacctcc aacacgggcg cggccgggtc agactactcc gccgcgtcgc
    18121 ccaacgcgtc gcgcgccgtc accgagagcg agtcgctcgc ccgcgagatc aaggacggga
    18181 cgctgacggg cttcgtcggc aacggcacga gcctgacggg cgaagcgagc ttcaagggga
    18241 tgctccgcgt cgatggccac ctgtcgggcc gcgtcagctc ctcggacggc acgctcatcg
    18301 tctcgaccaa cgggcaggtg gacgccaacg tcgaagtggc cgtcgcgcaa atctacggca
    18361 ccgtcaacgg cgacatcatc gcctccaagc gcatcgagat gggccgcgtc gccaaggtca
    18421 cgggcaacgt ccagacgccg gcgctcgtca tcgagaacgg cgcgctcttc gagggcagtt
    18481 gccgcatgat gcaacagcgc gaggaactgg atcggccggc ctccaggccc gagacctcga
    18541 cggccgcctc cgccgcctcg acctccaact cgtcttcaac ctccggctcc tcttcgacct
    18601 ccccctacgc ctccgcggcc aaaaacgaca agagcgacaa agcccccggc tcgacctctt
    18661 cgtccggctc cggcgtgacg accgccaccg acgcggtgag ttagacgcac cgcgcgtctt
    18721 catcacttta aagcttctcg cgcggcgccg ttcgcgaaat ggcgcgtcgc gcgagaagct
    18781 ttgtcgtgta ggccggccgc gcggcgggac gtgcgcccgt taggggcggc gcgcgcgctg
    18841 tgctagaatt ttttcgagtc gaaagactgt agactctcgc ccgagaggtt ctcaaaaaaa
    18901 gtgtcaaacg cgagtcgagc cgcgacgcat gagcgagccg gggacgcgtc cggccggcgg
    18961 ccgccctctg taaaaatttc atcgaccaag aaggacgaaa gccgaatatg agcgccatca
    19021 ttgaccagcc gcccgccccc ccgcaggagc gtcaacacga ggaggtcgag accgtcacca
    19081 tccgcttcgc cggcgactcc ggcgacggca tgcagttgac cggcacacag tttacgaaca
    19141 cctcggccgt gctcggcaac gacatctcga cgctccccga cttccccgcc gagattcgcg
    19201 cgcccgccgg cagtcttccc ggcgtctccg gcttccagct caacttctcc agccgcgaca
    19261 ttcgcacgcc cggcgaccag cccaacgtgc tcgtcgccat gaacccggcg gcgctcaaag
    19321 tgaatttgtc cgacctcgaa gacggcggca ccgtcatcat caacaccgac gagttcaaca
    19381 aggacaacct gaagaaggcc aactacgcca cgaacccgct cgaagacgac acgctgcgcg
    19441 gctaccgcgt ccaccgcctg ccgatcacca cggtcaacct gcgcgcgctg caagaggccg
    19501 gcgtgaaact gtcgcgcaag gagatggatc gctgcaagaa cttcttcgcc ctcggcgtcc
    19561 tctactggct ctacgaccgg ccgatggagc agacgctcga atggatcaag gcgaagttct
    19621 cgaagctgcc cgaggtggcg cgcgccaacg aagtggcctt gagagtcggc tacaacttcg
    19681 ccgacacgac cgaggtcttc accacccact acacggtcaa gaaggcggcg attgcgcccg
    19741 gcaagtaccg caagatcacg ggcaacgagg ccaccgccat cggcttcgtc gcggcttcgg
    19801 agctttcggg ccgcccgctc ttctacggct cctaccccat cacgccggcc tccgacatcc
    19861 tgcacgagct ggcgcgccac aaaaatttcg gcgtcaagac ctttcaggcc gaagacgaga
    19921 tcgccgccgt ctgcgccgcc atcggcgctt ccttcaccgg ccacatcgga ctcactggaa
    19981 cgtcggggcc gggcgtcgcc ttgaagcagg aggccatcgg actcgccgtg atgacggagc
    20041 ttccgctggt catcatcaac gttcagcgcg gcggcccctc gacgggcttg ccgacgaaga
    20101 ccgagcaggc cgatctcttt caggccgtct ggggccgcaa cggcgagtgc cccgcgattg
    20161 tgattgcgcc cgcgacaccg gccgactgtt tcacgatggc gattgaggcc gtccgcctcg
    20221 ccttcaagta catgtcgccg gtcttctatc tctcggacgg ctacctcgcc aacggcgccg
    20281 agccgtggct catccccgac atcgacgccc tgccgaagat cgacgtgcgc ttcgccgacg
    20341 accccgcctc cttcatgccc tacgagcgcg acgaccgcac gctctcgcgc ccctgggcca
    20401 tccccggcac gcccggactc gaacaccgcg tcggcggcat cgagaaggag cacctgacgg
    20461 gcaacgtcaa ctacgacccg gagaaccacc acttcatggt caagctccgg caggccaaag
    20521 ttgaccgcgc cgtgcaggac gtgcccgacg tggaggtcta cggtgagcgc acgggcaagg
    20581 tgctcgtcct cggctggggc tcgacgtatg ggtcgatcac ctcggccgtc gaacgcctgc
    20641 gcgaggaagg caagccggtc tccgcggcgc acctgcgcca cttgaatccc ttccccgcca
    20701 acctcggcga agtgctcggc aacttcgaga ccgtcatcat ccccgagatg aacctcggtc
    20761 aactctgcac gatgatccgc gcacgctatc tcgtcgatgc ccagcccttc tccaaggtca
    20821 agggccgccc cttccagatt cgcgagatca tcagcaaggt cgaggagtac ttgtaagcgt
    20881 cgggcaggtc gaaagcgatg agcaccacga ctcagttgac gactgcggaa gaactcctga
    20941 agttgccgcg cgggcgcttc cgctatgagc tcgtgaatgg agagctgaga cagatgtcac
    21001 cggcgggcca gaaccacgga aaaatcgcgg cgcgtttgac cgtcgctctc ggccagcacg
    21061 tcgaagagaa aaatctcggc gaggtctacg cggccgagac gggattcaaa ctcagaacga
    21121 gtcccgacac ggtgcgggcg ccggacgcga gtttcgtcgc gcgcactcgt gccgatgagg
    21181 tgggtgagtc gaagggttat ttccccggcc cacccgactt ggccgtcgaa gtcgtaagcc
    21241 ccgacgacac ggtcagagag gtcgaggaga aggtggccga gtggcttgag tacggaaccc
    21301 ggctcgtgtg ggtcgtcagc ccgaaactcc acgccgtcac cgtctaccgc tcgctcaccg
    21361 acatcgagac gctcacggag aacgacgccc tcgacggaga gacggtcgtg ccgggtttca
    21421 gttatccggt cgccaagctg ttcgcgggtc ggagttcctg attgagctac gcgcgcgcgt
    21481 aaagagtaaa cgggagtcag tcccttttgt gaaatcattc ggagaggatt catcgagatg
    21541 agcaccagcg gcaacgggag taacgggaac aaaaaagttg ataagagcgc ggcgggcatc
    21601 ggggggaacg tctcgggcgg cgggccgtcg ccggagagcg agccggggtc ggtcgtcgcg
    21661 ggcgcccccg cccctccgcc gggcaagctg acgaaggccg acttctcctc cgatcaggag
    21721 gtcaggtggt gtccgggctg cggcgactac gccatcctca acaacatcca gaaggtgatg
    21781 cccgaactcg gcatcccccg cgagaagatc gtcttcgtca gcggcatcgg ctgctcctcg
    21841 cgcttcccct actac
//
LOCUS       DASVES010000291         6575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1240181, whole genome shotgun sequence.
ACCESSION   DASVES010000291 DASVES010000000
VERSION     DASVES010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240181"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..520
                     /locus_tag="VF240_11765"
     CDS             197..520
                     /locus_tag="VF240_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7175927.1"
                     /translation="MNYSTMTPAEFTGRKERGDDLLLIDVREPEEFELARVEGAELLP
                     LSRFHEWADTLDPARETVVMCHHGIRSAQVCAVLARQGFARMHNLAGGIDRWSAEVDR
                     RVPRY"
     gene            615..1055
                     /locus_tag="VF240_11770"
     CDS             615..1055
                     /locus_tag="VF240_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX7175928.1"
                     /translation="MTVEEHNGAAYTEAHAAAYPIEYLAGIDLYNAGEFHAAHDAWEE
                     RWVDEAGPREKLFLQAMIQSAVVFHHLEIGRPGAARRMYQAATEKFARLGCERFMSLD
                     LADYQAQLERALSWLLTAADPREVTPPQFVPPIIRLLPEVMEYE"
     gene            1055..1675
                     /locus_tag="VF240_11775"
     CDS             1055..1675
                     /locus_tag="VF240_11775"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX7175929.1"
                     /translation="MNAENNRETISSSASMVEHAQRLGVLPAAARAGAGTGWARVRAA
                     FVADARLLLIALWLGAAVFFSFAVAPSAFAVLPARELAGAVVSRTLAIVNTGGAVIGL
                     LLLLSLPLDRGARSRRAVAGEAASLLILTALCAAGQWVVAARMQALRAQMGRPIDELA
                     ATDPLRVAFNSLHVYSVWALVAAMLAGAAALLLIARRTRMRERDKG"
     gene            1776..2612
                     /locus_tag="VF240_11780"
     CDS             1776..2612
                     /locus_tag="VF240_11780"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEX7175930.1"
                     /translation="MSSVRRTPQVEIIERMDEWRDRYARWKLTNEMRGGEVGAHYPWV
                     ESTRAPFTPARRALPMLNLALITSAGAYIDGTEPFDTELYGGDPSFREIPIEVGAEDL
                     KWSARGYDPAAVREDMNSQVPVARLQEFEGNGIIGRLNPVFWSFCGFAPNAARFAETA
                     LARLVERVTRYGAQAALIVPASRLCHQTMSLAARALEAARIPTMTLGVERETFDRVRP
                     PRAVFYAGDYGSVAGRPGWPEHQRRILDEALRLIEPFDQVGVHKLSVELETAVEVERG
                     EK"
     gene            complement(2714..6256)
                     /locus_tag="VF240_11785"
     CDS             complement(2714..6256)
                     /locus_tag="VF240_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7175931.1"
                     /translation="MRPDTLQHYDEIRDATAPPTPAPSDASAEQLEALAALAAATADS
                     REVERLLEGLADTITRLTDYRTCMVALFTDEPPYHPRVLSYSRNIPPEYAERVAAGSY
                     PREQVMRLVEESVRIEVGQLGFAAYFPPSHYDLQGSIFADRYKAEVERAVPADPTAAR
                     WSESDELFVPLSTRGGEFLGVISLDDPRSGAAPERRSVLPVVAFARQAAQLIERQRDA
                     DALAQQVEREALVNRITRAVRRSLEPEEVFRAAVEEMGVHLSADRCTLYMLDREAGVA
                     RNVAEFDAHGVEPAGGTFLVAPMRLLTERIIRHGVVTFDDVTADPSIRPVYEAILKRA
                     GTRSIMYAAVTVGEEVHGAFAISTLRERHWRESDVALARAVADQTGIAIRQAELYQRA
                     EATSAREALINRLSKAVRASLDLPEVLHAATHELGAALRASRVYLRPYDPAREEATPV
                     EHEYLAPGVASIGNSRISYLNPIGQFLIRNLRPLVIDDAEHPPAESPDLATQVRDHAA
                     RYGVSSMIFCPLIVQGDFRGALCIHQTGGARHWGRDDVALVEAVAAQLAVGIAQAELF
                     EMVARGKQTWEATFDAMSDGVFIFDAGRQLVRVNRAGAEMESAPPRELLGRRCCDILR
                     SGESHGCLVERVMTERRGLTLEYTPERLARPLLVTAEPLRLSGREIGSVCTVRDLTEL
                     RQFERLARERQTLLEHVLESARESICALDPEGRFMWCNSGATAMLGYAQDDLIGRHFS
                     EVMDPADEELARERFALALGGQPQTTEARIVTRDGHVRYSVSDTTPLVIDGRTTGVLS
                     ISRDVTEKRQERERAAQADKLRALGQLASGVAHDFNNALAAIMGRAELLRRSAFDPAA
                     ARGLDVILTAAEDAAATVRRIRAFARQAPGEEMATVDVAELLRDSVEITRTRWENEAR
                     ARGLSYDVRLEACEECAASGNASELREVFVNLIVNALDAMSTGGRLTVGCRPEGEWVR
                     VLFTDTGAGMTEEVRARVFEPFYTTKGAHGTGLGLFVSYGIIERHRGQIAVESDEGRG
                     STFAIMLPRVADAVVVAAPQRGPARVAGVLPLRVLVVDDEEVVRETLADMIGVLGHYA
                     TPAASGREALDLLDGAEFDVIFSDLSMPGMDGWELAREVRRRRHRARICIVTGYGKDT
                     APPDADGEQSPADAVIGKPFNFEQVEETLARFKD"
BASE COUNT         1033 a         2172 c         2327 g         1043 t
ORIGIN      
        1 cgccctttcg ctttagagcg atgttcactt gagtggaagc gaagtagatt caaccctgcg
       61 gcgctaactc tgcgtcctgc ggcgcctctg cggtgaatct atttccctct ccctcaactg
      121 ataactgata taaagcgcgc cgcgtttgcg ttctcacggc cgggttggta gctttgggcg
      181 cggaggtgag cggcagttga actactcgac gatgacaccg gcggagttta ccgggcgcaa
      241 ggagcgcggc gacgacttgt tgttgatcga cgtgcgcgag ccggaggagt tcgagctggc
      301 gcgcgtcgag ggcgcggaac tgctgccgct cagccgcttc cacgagtggg ccgacacgct
      361 cgaccccgcg cgcgagaccg tcgtcatgtg tcaccacggc atccgcagcg cgcaggtctg
      421 cgccgtcctc gcccgacagg gcttcgcgcg aatgcacaat ctcgccggcg gcatcgaccg
      481 ctggtcggcg gaagttgacc ggcgcgtccc gcgctactaa tagaagtgat gagtgatgag
      541 tgatgagtga taagaaaatc cgtttttctc tttcttatca ctcatcactc atcactcatc
      601 acttgttaat tagcatgact gttgaagaac ataacggcgc ggcttacacg gaggcgcacg
      661 ccgccgcgta tccaatcgaa tacctcgcgg gtatcgacct ctacaacgcg ggcgagttcc
      721 acgcggcgca cgacgcctgg gaagagcgct gggtggacga ggcggggccg cgcgagaagt
      781 tgttccttca ggcgatgatt cagtcggccg tggtctttca tcatctggag atcgggcgcc
      841 ccggcgcggc gcggcggatg taccaggcgg cgacggagaa gttcgcgcgc ctcggctgcg
      901 agcgttttat gtcgctcgac ctcgcggact atcaggcgca actcgaacgc gcgctctcgt
      961 ggctgctcac ggcggccgac ccgcgcgagg tcacgccgcc gcagtttgtg ccgccgatca
     1021 tccgcctcct gccggaagtg atggagtacg agtgatgaac gccgaaaaca accgcgagac
     1081 gatctcctcc tccgcctcga tggtcgagca cgcgcagcgg ctcggcgtcc tgcccgccgc
     1141 cgcccgcgcc ggcgccggga ctggctgggc gcgagtgcgc gctgcgttcg tcgccgacgc
     1201 gcgcctgttg ctcatcgcgc tgtggctcgg cgcggctgtc ttcttcagct tcgcggtggc
     1261 gccctccgcc ttcgcggtgc ttcccgcgcg cgagctggcc ggggcggtcg tctcgcgcac
     1321 gctcgccatc gtcaacacgg gcggcgccgt catcggcctg ctgctcctgc tgagcctgcc
     1381 gctcgaccgg ggcgcgaggt ctcggcgcgc ggtagcgggc gaggccgcgt cgctgctcat
     1441 cctcaccgcg ctgtgcgcgg cgggccagtg ggtcgtcgcc gcgcggatgc aggcgttgcg
     1501 cgcacagatg ggacgaccca tcgacgagct ggccgccaca gacccgctgc gcgtcgcctt
     1561 taacagtctg cacgtctact cggtctgggc gctcgtcgcg gcgatgctgg cgggagcggc
     1621 ggcgctactg ctcatcgccc ggagaacaag gatgagggaa agggataagg gatgagggat
     1681 gagggatgaa gtgaaagctt tattcatccc tcatccctta aattgataag ccccgcgttc
     1741 tgaattaaaa tccgcgcgac cggaagacaa ctaaaatgtc gagtgtgagg agaacgccgc
     1801 aggtggagat catcgagcgc atggacgaat ggcgcgaccg atacgcgcgc tggaagctga
     1861 cgaacgagat gcgcggcggc gaggtcggcg cgcactaccc ttgggtcgag agcacccgcg
     1921 cgccattcac gcccgcgcgc cgcgcgctcc cgatgctcaa cctcgcgctc atcacctcgg
     1981 ccggcgccta catcgacggc accgaaccct tcgacactga actctacggc ggcgaccctt
     2041 ccttccgcga gattcccatc gaggtcgggg ccgaagattt gaagtggtcg gcgcgcggct
     2101 acgacccggc ggcggtgcgc gaggacatga actcgcaggt gcccgtcgcg cgcttgcagg
     2161 agttcgaggg caacggcatc atcggacgtt tgaaccccgt cttctggagc ttctgcggct
     2221 tcgcgcccaa cgccgcgcgc ttcgccgaga cggcgctcgc ccgcctcgtc gagcgcgtca
     2281 cgcgctacgg ggcgcaggcc gccctgatcg tccccgcctc gcgtctgtgt caccagacga
     2341 tgtcgctcgc cgcgcgcgcc ctcgaagccg cgcgcatccc gacgatgacg ctcggcgtcg
     2401 agcgcgagac cttcgaccgc gtccgtccgc cgcgcgccgt cttctacgcc ggcgactacg
     2461 gctccgtcgc gggtcgcccc ggctggcccg agcaccagcg gcgcatactc gacgaagccc
     2521 tgcgcctcat cgagccattc gatcaggtgg gcgtccacaa actctctgtc gaactggaga
     2581 cagccgtcga agtcgagcgc ggcgagaagt aaaaagcaaa ggggtaaggg gtaaagggaa
     2641 aggggtaagg gaaaagacaa gttcttctcc cttaccccgt cccctttacc ccttacccct
     2701 ttcagagtca ccctcagtct ttgaagcgcg ctaatgtttc ttcgacctgc tcgaagttga
     2761 agggcttgcc gatgacggcg tcggccgggc tttgttcgcc gtcggcgtcg ggtggggcgg
     2821 tgtctttgcc atagccggtg acgatgcaga tgcgcgcgcg gtggcggcgg cggcgcacct
     2881 cgcgcgccag ttcccagccg tccatgccgg gcatggagag gtcggagaag atcacgtcga
     2941 actccgcgcc gtcgagcaag tcgagcgcct cacgccccga agcggccggt gtcgcgtagt
     3001 gtccgagcac cccgatcatg tcggccagcg tttcgcgcac gacctcctcg tcgtccacga
     3061 cgagcacgcg caaggggagc acgcccgcga cgcgcgccgg ccctcgctgc ggcgcggcga
     3121 cgactactgc gtccgccacg cgcggcagca tgatggcgaa agtgctgccg cgcccctcgt
     3181 ccgactcgac cgcgatctgc ccccgatgcc gctcgatgat gccgtagctg acgaagaggc
     3241 cgaggcccgt accgtgcgcc cccttcgtcg tgtagaaagg ctcgaagacg cgcgcgcgca
     3301 cctcctccgt catccccgcg cccgtatccg tgaagagcac acgcacccac tcgccctcgg
     3361 ggcggcagcc gaccgtgaga cggccgcccg tggacatcgc gtcgagggcg ttgacgatga
     3421 gattgacgaa gacctcgcgc agctccgagg cgttgccgct ggccgcgcac tcttcacagg
     3481 cttcgagacg cacgtcgtag gagaggccgc gcgcacgggc ctcgttctcc cagcgcgtgc
     3541 gggtgatctc gaccgagtcg cgcaaaagct cggccacatc gacggtggcc atctcctcgc
     3601 cgggcgcctg gcgggcaaag gcgcggatgc ggcggacggt ggcggcggca tcctcggcgg
     3661 cggtgaggat cacgtcgagg ccgcgggcgg cggccggatc gaaggccgaa cggcgtagca
     3721 actcggcgcg gcccatgatg gccgcgagcg cgttattgaa gtcgtgggcg acgcccgagg
     3781 cgagctgacc gagggcgcgc agcttgtcgg cctgcgcggc gcgctcgcgc tcctgccgtt
     3841 tctccgtcac gtcgcgtgag atggagagca cgccggtagt gcggccgtcg atgacgagtg
     3901 gggtcgtgtc ggagacggag tagcggacgt gtccgtcgcg cgtgacgatt cgcgcctcgg
     3961 tcgtctgcgg ctggccgccg agggcgagcg cgaaacgctc gcgcgccagc tcttcgtcgg
     4021 ccgggtccat gacctcggag aagtgtcggc cgatcaaatc gtcctgcgcg tagcccagca
     4081 tcgcggtcgc gccgctgttg caccacatga agcgcccctc ggggtcgagc gcgcagatcg
     4141 actcgcgcgc gctctccagc acgtgctcga ggagcgtctg ccgctcgcgg gcgaggcgct
     4201 cgaactgccg cagctccgtg aggtcgcgca ccgtgcagac gctgccgatc tcgcgcccgc
     4261 tcagacgcaa cggctcggcc gtgacgagca gcgggcgggc gagacgttcg ggcgtgtatt
     4321 cgagcgtgag gccgcgcctt tcggtcatca cgcgctcgac gaggcagccg tgcgactctc
     4381 ccgagcgcag gatgtcgcag cagcgccggc ccaaaagctc acgcggcggg gccgactcca
     4441 tctccgcccc cgcgcggttg acgcgcacca gttgccgccc ggcgtcgaag atgaagacgc
     4501 cgtccgacat cgcgtcgaag gtcgcctccc atgtctgctt gccgcgggcg accatctcga
     4561 acaactcggc ctgcgcgatg ccgacggcga gctgcgcggc gacggcctcg accaaggcca
     4621 cgtcgtcgcg gccccagtgg cgcgcgccgc ccgtctggtg aatacagagc gcgccgcgga
     4681 agtcgccctg cacgatgagc gggcagaaga tcatcgaact cacgccgtag cgcgccgcgt
     4741 ggtcgcgcac ctgcgtggcg aggtcgggcg actcggcggg tgggtgctcc gcgtcgtcga
     4801 tgacgagggg gcgcaggttg cgaatgagga actggccgat ggggttgagg tagctgatcc
     4861 tgctgttgcc gatggaggcg acgcccggcg cgaggtattc gtgctcgacc ggcgtcgcct
     4921 cttcgcgcgc cgggtcgtag gggcgcaggt agacgcgcga ggcgcgcagg gcggcgccga
     4981 gttcgtgcgt cgcggcgtgc agcacctcgg gcagatcgag cgaggcgcgc acggccttgc
     5041 tcaggcggtt gatgagcgcc tcgcgcgccg aagtcgcctc ggcccgctga tagagttcgg
     5101 cctggcggat ggcgatgccc gtctggtcgg ccacggcgcg cgccagcgcc acgtcagact
     5161 cgcgccagtg tcgctcgcgc agggtcgaga tggcgaaggc gccgtgcacc tcctcgccca
     5221 cggtgacggc cgcgtacatg atcgagcgcg tgcccgcccg cttcaggatc gcctcataga
     5281 cgggacggat cgacgggtcg gccgtcacgt cgtcgaaggt gacgacgccg tggcggatga
     5341 tgcgttcggt gagcagacgc atcggcgcga ccagaaaggt gccgccggcc ggctcgacgc
     5401 cgtgggcgtc gaactcggcc acgttgcgcg ccacgccggc ctcgcggtcg agcatgtaga
     5461 gcgtgcagcg gtcggccgag aggtggacgc ccatctcctc gacggccgcc cggaaaacct
     5521 cttcgggttc gagcgagcgg cgcacggcgc gcgtgatgcg gttgaccagc gcctcgcgct
     5581 cgacctgctg ggcgagggcg tcggcgtcgc gctggcgctc gatgagctgc gccgcctgac
     5641 gtgcgaaggc cacgacgggc agcacggagc ggcgctcagg ggcggcgccc gagcgcgggt
     5701 cgtcgagcga gatgacgcca aggaattcgc ccccgcgcgt ggagagcggc acgaagagtt
     5761 cgtcggactc cgaccagcgc gcggcagtgg ggtcggccgg gacagcgcgc tccacctcgg
     5821 ccttgtagcg gtcggcgaag atgctccctt gcaggtcgta gtgagagggc gggaagtagg
     5881 cggcgaagcc gagctggccg acctcgatgc ggacggactc ttccaccaga cgcatgacct
     5941 gctcgcgcgg gtacgagccg gcggcgacgc gctcggcgta ttcgggcggg atgttgcgcg
     6001 agtaggagag gacgcgcggg tggtagggcg gctcgtccgt gaagagggcg accatgcacg
     6061 tccggtagtc cgtcagacgc gtgatcgtgt cggccagacc ttcaaggagg cgttcgacct
     6121 cgcggctgtc ggccgtcgcg gcggccagcg ccgccagcgc ctcaagctgt tcggcggagg
     6181 cgtcagaggg cgcgggcgtc ggcggcgccg tggcgtcgcg gatttcgtcg tagtgttgaa
     6241 gagtgtcggg gcgcatgcga caagttcggg gccgtcggtg cgaggaacgg ctgcaagagg
     6301 actccggggg acgacgacac gcggcatggg aggcggcaca cacttctgcc gaaacatcga
     6361 cgccggcggc aagtctcaga attcaatcaa ggatggggcg aactcttacg ctttgcgcgc
     6421 gccggctggg gtcagaggag gccgagacca cgcggggaaa gtatagcaaa gcggcgcagg
     6481 ccctgtcatg gaaagtttac ggcggttttt aattgccccg accgattaga ggcgggccat
     6541 gcccgccggc tcaagactcg gtgcttgagc cggcg
//
LOCUS       DASVES010000292         4811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_392807, whole genome shotgun sequence.
ACCESSION   DASVES010000292 DASVES010000000
VERSION     DASVES010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392807"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VF240_11790"
     CDS             complement(<1..349)
                     /locus_tag="VF240_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175932.1"
                     /translation="MNQSLKERLKENLPFSAKFLLKRLLVASVGAIVRLAASAVRFKN
                     RLLDAAVKHGLDLERAWNVTPRDRAGAQQSQPPPFGASDFLFLTNMIAGASAKSAASV
                     ERPVRASIIIPVFN"
     gene            complement(496..1710)
                     /locus_tag="VF240_11795"
     CDS             complement(496..1710)
                     /locus_tag="VF240_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7175933.1"
                     /translation="MDTALRIENVGKRYRIGALHPGYATLREALGEALLAPFRRRGPD
                     AHAHVWALREVNLEVRQGELVGVIGRNGAGKSTLLKVLSRVTKPTTGRENVFLSGAIL
                     GMHRAEIERKFDEIVAFSETERFIDTPVKHYSSGMYVRLAFAVAAHLEPEILMMDEVL
                     AVGDAAFQQKCLDKMHDIRQQGRTILFVSHNMPAVVRLCRRVVLLREGRVAADGAAQP
                     VVSEYLSASWNVEAERAWDVGDEAPGDDVVRLRRVRVRDEAGETAAAFDLRWPFGIEV
                     TYDVLRAGEFLTPHVELHNEEGVRLFSSHDTGEEWRERARSAGRFVSTAWVPGNLLAE
                     GHLLAHVSLMSHRPANVLRANAPRVVALQLIDGGADDSARGDYTGPMPGVVRPLLRWT
                     TSADDGIESSAH"
     gene            complement(1799..2728)
                     /locus_tag="VF240_11800"
     CDS             complement(1799..2728)
                     /locus_tag="VF240_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7175934.1"
                     /translation="METKARESSSALGRADASPAGAHAAAGPQSAAAGAAHAPPVTVI
                     LSEESGVQLGLSDLWRYRELLYFLTWRDIKVRYKQTALGAAWAVIQPLAQMLLFTLVF
                     HRFAGLGSGDMPYPLFAYAGLLVWTFFANAVLNSTHSLVTNTNLITKVYFPRVFIPAA
                     AVAAGLVDLGIASVILAGLVAYYRIAPGWGAALLPLFIALAVLLSLAVGMLISATTVK
                     YRDLRHALPFVMQFWMFATPVIYPSTVVPDGWRQVLALNPATGILEGFRASLTGGAID
                     RTLTVTAAVETVLLLALSFYVFRRNEETFADVI"
     gene            3051..3929
                     /locus_tag="VF240_11805"
     CDS             3051..3929
                     /locus_tag="VF240_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7175935.1"
                     /translation="MNFVHTLKRVVPRGLHPRLGAARRLVLSLPSRTAAQKARLLEDP
                     ALGAGERELLQRASSRVHYQDGMYNGDGAHYFKVGLSALRCIDEAVESAGVAEVRRIL
                     DLPCGYGRVLRFLVHRFPAAQITACEIDRAAVDFCVREFGAAPAYSSADLDALDFGAR
                     FDLIWCGSLATHLSADPTLALLKLFRRHLAPAGLVLFTTHGDYVARRLPTKDFDYGLT
                     DEQITRITDAYARDGHAYTDYPDRASYGVSLTSPAWVREQARRAGRLREVYFKPRGWD
                     DHQDVYGFVLEGEREL"
     gene            complement(4159..>4811)
                     /locus_tag="VF240_11810"
     CDS             complement(4159..>4811)
                     /locus_tag="VF240_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175936.1"
                     /translation="EFLRARVAALEARPRAVLAYGGAYTIDEHDRIIDSTTDWARYAD
                     GDVRRMLMEILAPLSPTVVYRRAALEGRRWNERARLEDYEMYLRLSAAGEFAFDPRVL
                     SAWRVHDHNASEDLELMMHERLAAQTVVGPTLGLREEELGAYRALARFRSAEEFIRRG
                     RKRRAAGLILSNPRGVPSAAAGARTLLKFLTPYRVLSWRRRRERERAFGRYGSLDV"
BASE COUNT          842 a         1585 c         1585 g          799 t
ORIGIN      
        1 tgttgaagac ggggatgatg atggacgcgc ggacgggtcg ttcgacggac gcggctgact
       61 tggcgctcgc gcccgcgatc atgttcgtga ggaacaggaa atcactcgcg ccgaagggcg
      121 gcggttgtga ctgttgtgcg ccggcccggt cccgcggcgt gacgttccac gcgcgctcaa
      181 ggtcgaggcc gtgtttgacg gcggcgtcga gcaggcggtt tttgaaacgg acggccgatg
      241 cggcgaggcg gactatagcg ccgaccgatg cgaccagcag gcgcttcagg agaaacttcg
      301 cggagaaggg caggttctcc ttgagccgct ctttaagtga ttgattcatt gaatcatcga
      361 ttcattgact catttaaatc agtaccgcga gcggtagcga gcgggtctcg cgtgcgccag
      421 gcaagccgct catcaccgcc gcacccgctc gctaccgctc gcggtactgt gcgtgacggc
      481 gactccctca ttgaatcaat gagccgacga ttcaatgccg tcgtccgcgc tcgtcgtcca
      541 gcgcagcagc gggcgcacga cgccgggcat cggccccgtg tagtcgccgc gcgccgagtc
      601 gtccgcgccg ccgtcgatta gttggagggc gacgacgcgc ggggcgttgg cgcgcaggac
      661 gttcgcgggg cggtgcgaca tcaaagagac gtgcgccagc aggtggcctt cggcgagcag
      721 gttgccgggc acccaggccg tgctcacgaa gcgaccggcc gagcgcgcac gctcgcgcca
      781 ctcttcgccc gtgtcgtgcg agctgaacag gcgcacgccc tcctcgttgt gcagctcgac
      841 gtgcggtgtc agaaactcgc ccgcgcggag cacgtcgtaa gtcacctcga tgccgaaggg
      901 ccagcgtagg tcgaaggccg cggccgtctc tcccgcctcg tcgcgcacgc gcacgcgccg
      961 cagccgcacc acatcgtcgc ccggcgcttc gtcacccaca tcccacgcgc gctcggcctc
     1021 gacgttccag ctcgcgctca ggtactcgct cacaacgggc tgcgccgcgc cgtcggcggc
     1081 gacgcggccc tcgcggagta acacgacgcg gcggcacaga cgcacgaccg ccggcatgtt
     1141 gtgggagacg aagaggatgg tgcggccctg ttggcggatg tcgtgcatct tgtcgaggca
     1201 cttctgctgg aaggccgcgt cgccgacggc cagcacctcg tccatcatca gaatctccgg
     1261 ctccagatgc gccgcgacgg cgaaggcgag gcggacgtac atgcccgagg agtagtgctt
     1321 gacgggcgtg tcgatgaagc gttcagtctc ggagaaggcg acgatctcgt cgaacttgcg
     1381 ttcgatctcc gcgcggtgca tgccgaggat ggcgccggag aggaagacgt tttcgcgccc
     1441 cgtcgtcggc ttcgtcacgc gcgacagaac tttcaaaagc gtgctcttgc ccgcgccgtt
     1501 gcggccgatg acgccgacca gctccccctg ccgcacttcc agattcacct cgcgcagcgc
     1561 ccagacatgc gcgtgagcgt cggggccgcg ccggcgaaaa ggagccagca gcgcctcgcc
     1621 cagcgcctcg cgcagcgtcg catagccggg atgcagcgca ccgatgcggt aacgcttccc
     1681 gacattctcg atgcggaggg ccgtgtccat ttaaaaaccg tgataagtga tgagtgataa
     1741 gtgataagaa aaacagcctc cttcttatca cttatcactc atcacttatc actattcctc
     1801 atatcacgtc cgcgaatgtc tcctcgttgc gccggaagac gtagaaggag agggctaaga
     1861 gcaggacggt ttcgacggcg gccgtgacgg tcagtgtccg atctatcgcg ccgcccgtga
     1921 gcgaagcgcg gaagccttcg aggatgccgg tcgcggggtt gagcgcgagc acctgccgcc
     1981 agccgtcggg gacgaccgtc gaaggataga tgaccggcgt ggcgaacatc cagaactgca
     2041 tcacgaaggg caaggcgtgg cgcaggtcgc gatacttgac ggtcgtcgcc gagatgagca
     2101 tgccgacggc gagcgagagc agcacggcga gcgcgatgaa gagaggcaac agggccgcgc
     2161 cccagccggg cgcgatgcgg tagtaggcga cgagtccggc gaggatgacg gaggcgatgc
     2221 cgaggtcaac aaggccggca gcgacggccg ccgccgggat gaagacgcgc gggaagtaga
     2281 ccttcgtgat gagattggtg ttggtgacga ggctgtgcgt gctgttaagg acggcgttgg
     2341 cgaagaacgt ccacacgagg agtccggcgt aggcgaagag cgggtagggc atgtcgcccg
     2401 agccgaggcc ggcgaagcgg tggaagacga gcgtgaagag aagcatctgg gcgagcggct
     2461 ggatgacggc ccaggccgcc ccgagcgccg tctgcttgta acgcactttg atgtcgcgcc
     2521 aggtgaggaa gtagagcagc tcgcggtaac gccagaggtc ggagagtccg agctgcacgc
     2581 ccgactcttc ggagaggata acggtgacgg gcggcgcgtg cgcggcgcct gccgcggcgg
     2641 actgcggccc cgccgcggcg tgcgcgcccg ccggcgaagc gtctgcgcgt ccgagcgcgc
     2701 tcgaactttc gcgggccttc gtctccatcg cactcggctg ttggtgtgga tgctcggccg
     2761 gggcttcctg ggccgccctt ctcccggcgc tgctatcgag gctcaaggta cgggcaagtc
     2821 tagcggcgcg cgcgcgcgcg cgtcaaggaa ggcgtgtgct atcgttagct gttagcgatt
     2881 agctgttagc tgttagctaa aaactgtctg tggtcagtag tccgtggtca gttgtttacg
     2941 ttgcacggct tccggccttt ccgatgtcag cggcgagtcg gccccagaca agcaactgac
     3001 cactgaccac ggacaacaaa cagctcttag ttaacagcta aacttctctt atgaacttcg
     3061 ttcacactct caagcgcgtc gtgccgcgcg ggctccaccc gcgtctggga gcggcgaggc
     3121 gactcgtgct ctcgctcccg tcgcgcaccg ccgcgcagaa ggcgcgtctg ctcgaagacc
     3181 ccgcgctcgg agcaggggag cgcgagctgc tacagcgggc gagcagccgc gtccactatc
     3241 aggacgggat gtataacggc gacggcgcgc actacttcaa ggtcggactc tcggcgctgc
     3301 gctgcatcga cgaggccgtc gagagcgccg gggtcgctga agtccgccgc atcctcgacc
     3361 tgccgtgcgg ctacggacgc gtgctccgct ttctcgtcca ccgttttccg gccgcgcaaa
     3421 tcacggcctg cgagatcgac cgcgcggccg tggacttctg tgtccgcgag ttcggcgccg
     3481 cccccgccta ctcttccgcc gacctcgacg cgctcgactt cggcgcgcgc ttcgacctca
     3541 tctggtgcgg ctcgctcgcc acgcacctca gcgccgaccc cacactcgcc ctgctcaaac
     3601 tcttccgccg ccacctcgcc cccgccggcc tcgtcctctt caccacgcac ggagactacg
     3661 tcgcccgtcg tctgcccacg aaggatttcg actacggact caccgacgaa cagatcacac
     3721 gcatcaccga cgcctacgca cgcgacggcc acgcctacac cgactatccc gaccgcgcaa
     3781 gctacggcgt ctccctgacc tcgcccgcgt gggtgcgcga acaggcgcgc cgcgccggac
     3841 gcttgcgcga ggtctacttc aagccgcgcg gctgggacga ccatcaggat gtatacgggt
     3901 tcgtgttgga aggcgagcgg gaattgtagg ggcagggctt gtccctaccc gccgccgaca
     3961 gaagaattaa catggataga caggatgggc acgatcagaa atttaaaatc tcaaatctca
     4021 aatctcaaat ctgcaatcag tttttctttc tcctgcccac cctgtccatc catgttaatt
     4081 gagcctttcg cgagggaggg cagggacaag ccctgcccct acagctttcc gtcaacgcta
     4141 catcttcggc aacgcccttt aaacatccag cgagccgtaa cgcccgaagg cgcgctcgcg
     4201 ctcgcgtcgc ctccgccagc tcaacacgcg gtacggcgtc agaaacttga ggagcgtccg
     4261 cgcacccgcc gccgccgagg gaacgccgcg cgggttcgag aggatgagac cggcggcccg
     4321 acgcttccgg ccgcgccgga tgaactcctc cgcgctgcgg aagcgcgcga gcgcccggta
     4381 agcccccagc tcttcctccc tcaaacccag cgtggggccg acgaccgtct gcgccgccag
     4441 ccgctcgtgc atcatcaact ccaaatcttc gctcgcgttg tggtcgtgta cgcgccatgc
     4501 ggagagcacc cgcgggtcga aggcgaactc gcccgcggcg ctcagacgca agtacatctc
     4561 gtagtcttcg agacgcgcgc gctcgttcca gcgtcggcct tcgagcgccg cgcggcggta
     4621 gacgactgtg gggctgagcg gcgcgagaat ttccatgagc atccggcgca cgtcgccgtc
     4681 cgcgtagcgc gcccagtcgg tcgtggagtc gatgatgcgg tcgtgctcgt cgatggtgta
     4741 ggcgccgccg taggcgagca ccgcgcgcgg cctcgcttcg agcgcggcga cacgcgcgcg
     4801 taaaaattcg g
//
LOCUS       DASVES010000293         9248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_721903, whole genome shotgun sequence.
ACCESSION   DASVES010000293 DASVES010000000
VERSION     DASVES010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721903"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..374)
                     /gene="rpsU"
                     /locus_tag="VF240_11815"
     CDS             complement(174..374)
                     /gene="rpsU"
                     /locus_tag="VF240_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7175937.1"
                     /translation="MATVNVNQGESIESALRRFKRKVMSEEIIKDAKKHTFFMSRGQK
                     AKLKSAMARKRNKRKQRGFRRS"
     gene            complement(594..1610)
                     /locus_tag="VF240_11820"
     CDS             complement(594..1610)
                     /locus_tag="VF240_11820"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX7175938.1"
                     /translation="MNRTILNALLTLALTLALPHAAAAQQQTPAPQGSPQAQSAPRIE
                     PASLRVFDSKGNATTLDAVVEALSDADIVFVGETHDDAGAHLLEAELLRRAHARFALE
                     GNAKERRPVSLSMEMFERDVQIVLDEYLSGLISERHFLAASRPWKNYQTDYRPLVEFA
                     REHKLPVIAANAPARYVSRVSQHGPASLSELTAEARSWLPPLPFAPASRVYAEKFNAF
                     MGGAAARPSQLPAAPATTANTPPPQTAHGGALHLLDAQNLRDASMAHAIVEHLKRRER
                     ALVVHVNGTFHSEERLGVPEHVSRYRPKARTLVVTAVARADFDAREMSKLGDFIVLTR
                     PAAR"
     gene            1703..2266
                     /locus_tag="VF240_11825"
     CDS             1703..2266
                     /locus_tag="VF240_11825"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEX7175939.1"
                     /translation="MGEIFVSTDVEADGPVPGLNSMLSLGSAAYLPDKTLVATFEANI
                     ETLEGASGHPETMRWWTTQEAAWRACRENPRPAAEVMTDYLAWLKGLPGRPVFVAYPA
                     AFDFMYVCWYLNRFTGRNPFGWVALDIKTFAMAMLDTDFHDTVKSRMPPRWFDADLPH
                     THKALDDAIEQGALFCNMLAEHKSRES"
     gene            complement(2469..3599)
                     /locus_tag="VF240_11830"
     CDS             complement(2469..3599)
                     /locus_tag="VF240_11830"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7175940.1"
                     /translation="MELESLKTPSLLLDVERVRRNAERVSSRVREMDAALRPHVKTHK
                     CVEVARIQTGGHSGAITVSTLAEARAFLGHGFTDITYAVPIEPGKFDEAVSLARRCDR
                     FALITDDAETMRLLDDHARRAAVTLDVFLKVDCGYHRCGVEPRAREAVEIPRLISDSA
                     HLRFAGLLTHAGHSYHARSPEELLAIARTERDVTVELAERLRASGVAVPVVSIGSTPT
                     ITTVDHLKGVDEARPGNYIFFDAFQATLGSCGFDDCALTVLASVVHRDPARRKIIIDA
                     GAVALSKDRGAVEFDSTCGYGRVFDLEGRDMGLRVGGLSQEHGEIVAGGDAGGDAVER
                     LKVGSRVRVLANHSCLTAAQHAFYNVVEGGRVTDRWEIHRGW"
     gene            complement(3681..5798)
                     /locus_tag="VF240_11835"
     CDS             complement(3681..5798)
                     /locus_tag="VF240_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX7175941.1"
                     /translation="MSKLIRLVTILCALLLAASFFATARFAGRAQNLARRQYAPDLRR
                     ALDARTKTPDDQTHATTRAGAYAPQRDPRAPALRERTQTQIREGARRLPPARPDGGRR
                     ARAGLERNPSALSPHVLGSITPLRPGTPLSRVLHTSQFNLTNAGGTNEQYADQTGDLA
                     ADERTTFDTDGGSFDIALGRSGSRYEVFSSIDTRGTANTGDDVLTGVLLVGRDSNGDF
                     VRDPGTPPTFDLGRDFRMPSAVSVVAGVSATGREFVVVSSSGYYNSDNPNDPNNEPSP
                     GVVLLVRDFAGGGFDPARSRELVRVGDDRLFNANALALLPGGDLLIADFQSNEIRAVR
                     DTDNDLVPDTLDPTPFYTFPFSAGAEDAPLDIAANSRGVVFTHSAGNDSRLLAVYDTD
                     GDGYGDVDDIAVSGLFIDNNLVLHGLTVGRDGAVYLIEDATGEHDLPADGGNGGIPLV
                     QAFPDPSLNGLLRDGSIFALADDEFTQGLSGLALGVDTVFGAVGRLTMTNAASLQGDA
                     PSGGLAAIRGTGLARGLSGATEAEAAQRGVRVSVEGNSVPVLSFNDTQINVHIPVALG
                     SGDGSVVVTVGGIVIAAEDARIVNANPGLFTLPQTGAGEAVALLVSGNRYTRSPFPAS
                     FGAQPSVVALFGTGWRNSLPVSVTVGGRAAVVEYAGASGGFPGLEQINVRFPDNTPAG
                     PASVVVTTAGGATSRADVFITVQ"
     gene            complement(5957..8164)
                     /locus_tag="VF240_11840"
     CDS             complement(5957..8164)
                     /locus_tag="VF240_11840"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX7175942.1"
                     /translation="MRSLKSLLLPLLFLATAFTFTARASAADDVDENEYEEHARVMRV
                     SLMRGEVSLRRAGNVEWERAKLNLPLVEGDTLATGPDSRLEIQIDAHNFVRVGADSVL
                     RVVTLREEGIAVSLSEGAATLRLARFDKDKEYFELDAPRTTVAAERRGLYRVDVARDG
                     GVRVTVRDEGRARVYSESSGFTLRNNRSARLYIEDGEGDWELATASAFDEWDTWNDER
                     ERNLASRLRYEQRERYYDRDVWGAEELDSYGDWHHTSQYGWVWRPHVTVVNHYHNWAP
                     YRYGHWRWVHPYGWTWVADEEWGWAPYHYGRWVVYNNNWCWAPRGYGYEYRRAWWRPA
                     LVAFVYIPTSYGEHVAWYPLRHGQHDPRGRWGQRRPDRLAPLRARDAANLERVNPLLL
                     RGVTAVPARDFGADSLRGRAAAADIARRTVTGEPVRGRLPITPADVGRTSTFATPARV
                     GPRGEVRTGAGVAADSGAGARPGNREGLRINRSAPVTPPRDIPQRDTGAASRTPGVPL
                     DEELRRTRVLGGRELRFPSPTSDGADARDDDRGTGAVARPARPARVSPTERGLPAEGA
                     APSVRRPRTPGESERSFPSPGTSVPTERTRPAERERDDEGDAARPARPSRVGRPEVDR
                     EQPSDERPASRPRADERPEPRERVRPREEERLSPPVRHDPPPQREERPAPRHEPAPRQ
                     ETPPQRDSAPPPSNERPAAPPPQRQERPAPPSRDSARPARPARNREPVKSDEP"
     gene            8923..>9248
                     /locus_tag="VF240_11845"
     CDS             8923..>9248
                     /locus_tag="VF240_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175943.1"
                     /translation="MKHRTVTVFIVVLAALAAVPQAFEQFSSLKDAAGNRLTAGVWNV
                     FLSLHGQKVAGAPRAQQLPQTALLVRAAAPEGPTQIARREARPAKSLTASSSPLSATH
                     LRAEEA"
BASE COUNT         1517 a         2901 c         3350 g         1480 t
ORIGIN      
        1 cttcatgtcg cgcgggcaga aggccaaact caagtcggcg atggcgcgca agcgcaacaa
       61 gcgcaagcag cgcggcttcc ggcgtagcta gacgccgctc acaaaaaaca taagcgcggg
      121 cgcgggtcga ttgatttgac ccgcgcccgc gccctgttta ttgtgagcgg cgtttagcta
      181 cgccggaagc cgcgctgctt gcgcttgttg cgcttgcgcg ccatcgccga cttgagtttg
      241 gccttctgcc cgcgcgacat gaagaaggtg tgcttcttcg cgtccttgat gatctcttcc
      301 gacatcacct tgcgcttgaa gcggcgcagc gcgctctcaa tcgactcacc ctggttgaca
      361 ttgactgtcg ccattaataa atctccgttg ccagaaccga ggctgaaaat gtgcggaggc
      421 gcgtacgcgc ggccccgcga gacgttagca attcctgagt ctttaagtag ttaactactg
      481 ataatagaaa gcaacacctg cctctcgggt cgtaaccgac cggttattgt gccaagccgg
      541 ggcggttatg gcaagcgcgc aaaggtggcg gaagacccga gtggctcccc ggcttaccgg
      601 gcggcggggc gcgtcaggac gataaagtcg ccgagtttcg acatctcgcg cgcgtcgaaa
      661 tcggcgcgcg cgacggccgt cacaacgagc gtgcgggcct ttggacgata gcgactgacg
      721 tgctcgggga cgccgaggcg ctcctcgcta tggaaagtgc cgttgacgtg gacgacgagc
      781 gcgcgctcgc ggcgcttgag gtgctcgaca atggcatggg ccatcgaggc gtcgcggagg
      841 ttctgcgcgt cgagcaggtg cagggcgccg ccgtgcgccg tctgcggcgg gggcgtgttt
      901 gcggtcgtag ctggagctgc cggcaactgg ctcgggcgcg cggccgcgcc gcccatgaag
      961 gcgttgaact tctcggcgta gacgcgcgag gccggcgcga acggcagggg cgggagccag
     1021 cttcgcgctt ccgccgtcag ctcggagagc gacgcggggc catgctgtga gacgcgcgag
     1081 acgtagcgcg ccggggcgtt cgcggcgatg acgggaagct tgtgctcgcg ggcgaactcg
     1141 acgagcgggc ggtagtccgt ctggtagttc ttccacgggc ggctcgcggc gaggaaatgt
     1201 ctctcgctga tgaggccgga gaggtattcg tcgaggacga tctgcacgtc gcgctcgaac
     1261 atctccatgg agagagacac ggggcggcgc tctttcgcgt tgccctccag cgcgaagcgc
     1321 gcgtgtgcgc gccgcagcag ctcggcctcc aacaggtgcg cccccgcgtc gtcgtgcgtc
     1381 tcgccgacga aaacgatgtc ggcgtcgctc agcgcctcga ccacggcgtc gagcgtggtg
     1441 gcgttgccct tcgagtcgaa gacgcgcagc gacgccggct cgatccgcgg cgcggactgc
     1501 gcctgcggcg aaccctgcgg cgcgggcgtt tgctgctgcg ccgcggccgc gtgcggaagc
     1561 gcgagcgtca gggcgagcgt caggagcgcg ttgagaatag ttcggttcac gttcggattt
     1621 tttatcactt cggatgtata agagcaaacg gccgcgcggc gggcgcgggc gcggcgaacc
     1681 gtagacgacg aaagggcgtg tgatgggaga gatattcgtg agcacggacg tggaggccga
     1741 cggccccgtc cccggcctga actcgatgct cagcctcggc tcggcggcct acctgccgga
     1801 caagactctg gtcgccacct tcgaggccaa catcgagacg ctggagggcg cctccggcca
     1861 ccccgagacg atgcgctggt ggacgacgca ggaggcggcc tggcgcgcct gccgcgagaa
     1921 cccgcgtccg gccgccgagg tgatgacgga ttacctcgcg tggctcaagg gacttcccgg
     1981 ccgccccgtg ttcgtcgcct atccggccgc cttcgacttc atgtacgtct gctggtatct
     2041 caatcgcttc accggccgaa accccttcgg ctgggtcgct ctcgacatca agaccttcgc
     2101 catggcgatg ctcgacacgg acttccacga caccgtcaag agccgcatgc ccccgcgctg
     2161 gttcgacgcc gacctcccgc acacacacaa ggccctcgac gacgccatcg agcagggcgc
     2221 actcttctgc aacatgctgg cggagcataa aagccgtgaa tcgtaaatcg tgaatcgtga
     2281 atcgattgcc ggtcgtccga ctgcggctca tcattcaagt tgagccgccc tcgaccacga
     2341 ttgacgattg acgattgcgg cagggaacac ggatgagacg gatgagacgg atgagacgga
     2401 tgagcacgaa tccgtgtcaa tccatctcat ccgtcttatc cgtgttctcc tcgcggccgc
     2461 gaacgcgctc accagccgcg gtgtatctcc caacggtcgg tgacgcgccc gccttcgacg
     2521 acgttataga aggcgtgctg cgccgccgtc agacaagagt ggttggcgag gacgcgcacg
     2581 cgcgagccga ccttcagacg ttcgacggcg tctcctcccg cgtcgccgcc ggccacaatc
     2641 tcgccatgct cctgcgaaag ccccccgacg cgcaggccca tgtcgcggcc ttcgaggtcg
     2701 aacacccgac cgtagccgca agtcgaatcg aactcaactg cgccgcgatc cttcgagagc
     2761 gcgaccgccc ccgcgtcgat gattattttc cgccgcgccg ggtcgcggtg gacgaccgag
     2821 gcgaggacgg tcagcgcgca gtcgtcaaag ccgcagctcc cgagcgtcgc ctggaaggcg
     2881 tcgaagaaga tgtagttgcc gggtcgcgcc tcgtccactc ccttgagatg atcgaccgtg
     2941 gtgatggtcg gcgtcgagcc gatgctgacg acggggacag cgacgcccga cgcgcgcagg
     3001 cgctcggcca actccaccgt cacgtcccgc tcggtgcgcg cgatggcgag cagttcttcg
     3061 ggcgagcgcg cgtgatagga gtggccggcg tgtgtgagca gcccggcgaa gcgcaggtgc
     3121 gccgagtcgg agatgaggcg gggaatctcg acggcctcgc gcgcgcgtgg ctcgacgccg
     3181 cagcggtgat agccgcaatc gactttgagg aaaacgtcga gcgtgacggc cgcgcgccgt
     3241 gcgtgatcgt caagcagacg catcgtctcg gcgtcgtccg tgatgagcgc gaagcggtcg
     3301 caccggcggg cgagcgagac cgcctcgtcg aacttgcccg gctcgatggg gacggcgtag
     3361 gtgatgtcgg tgaatccgtg cccgaggaag gcgcgggcct cggcgagcgt cgagacggtg
     3421 atggcgcccg agtggccgcc cgtttggatg cgcgccacct cgacgcactt gtgcgtcttg
     3481 acgtgcgggc gcagggcagc gtccatctca cgcacacgcg acgagacgcg ctcggcattg
     3541 cgtcgcacgc gctccacgtc gagcaatagc gaaggcgtct ttagtgattc gagttccatt
     3601 gattctcagt aggacaggca atcctgcctg tcattgcaag taagcagaca ggcaagaatg
     3661 cctgtcctac tgagattcta tcactgcacg gtgatgaaga catcggcgcg actggtcgcg
     3721 ccgccggcgg tggtgacgac gacggaggcg gggccggcgg gagtgttgtc ggggaagcgg
     3781 acgttgatct gttcgagtcc ggggaagccg ccggaagcgc ccgcgtactc gaccacggcc
     3841 gcgcgcccgc cgacggtgac gctgacgggc agggaattgc gccagcccgt gccgaagagg
     3901 gcgacgaccg aaggctgagc gccgaagctc gcggggaagg gcgagcgtgt gtagcggttg
     3961 ccggagacga ggagcgcgac cgcttcgccc gcgccggtct gcggcagggt gaagagaccg
     4021 gggttagcgt taacgattct tgcgtcctcg gccgcgatga cgatgccgcc cacggtgacg
     4081 accaccgagc cgtcgcccga gccgagcgcg acgggtatgt ggacgttgat ctgcgtgtcg
     4141 ttgaaggaga gcacagggac gctgttgcct tcgacgctga cgcgcacgcc gcgctgtgcg
     4201 gcctcagcct cggtcgcgcc gctcaggccg cgcgcgagcc ccgtgccacg gatcgcggcg
     4261 aggccgcccg aaggcgcgtc gccctgaagg cttgcggcgt tggtcatcgt caggcggccg
     4321 actgcgccga agacggtatc gacgccgagg gcgaggccgg atagaccttg cgtgaactcg
     4381 tcgtcggcga gggcgaagat ggagccgtcg cgcagcaggc cgttgagcga ggggtctggg
     4441 aaagcctgga cgagtgggat gccgccgttg ccgccatcgg ccgggaggtc gtgctcgccc
     4501 gtcgcgtctt caatcaggta gacggcgccg tcgcgcccga cggtcaggcc gtggaggacg
     4561 aggttgttgt cgatgaagag gccgctgacg gcgatgtcgt ccacgtcgcc gtaaccgtcg
     4621 ccgtcggtgt cgtagacggc gagcagtcga gagtcgttgc cggccgagtg cgtgaagacg
     4681 acgccgcggg agttggcggc gatgtcgagc ggggcatcct cggctccggc ggagaagggg
     4741 aaggtgtaga agggcgtggg gtcgagcgtg tcgggcacaa ggtcgttgtc cgtgtcgcgc
     4801 acggcgcgta tctcgttcga ctggaagtcg gcgatgagca gatcgccgcc gggcaggagc
     4861 gcgagcgcgt tggcgttgaa gaggcggtcg tcgccgacgc ggacgagttc acgcgagcgc
     4921 gcggggtcga agccgccgcc tgcgaagtcg cggacgagga ggacgacgcc gggcgacggc
     4981 tcgttgttgg ggtcgttggg gttgtcggag ttgtagtagc cgctcgaaga gacgacgacg
     5041 aattcgcggc ccgtggcgga gacgcccgcg acgacagaga cggccgaggg catacggaag
     5101 tcgcggccga ggtcgaaggt gggcggcgtg ccggggtcgc gcacgaagtc gccgttcgag
     5161 tcgcggccga cgaggaggac gccggtgagc acgtcgtcgc cggtgttggc ggtgccgcgt
     5221 gtgtcgatgg aagagaagac ttcgtagcgg gaaccggagc ggccgagggc gatgtcgaag
     5281 gagccgccgt cggtgtcgaa ggtggtgcgc tcgtcggccg cgaggtcgcc cgtctggtcg
     5341 gcgtactgct cattagtgcc gccagcattt gtgaggttga attgcgacgt gtgcagcacg
     5401 cggctgagcg gggtgcccgg ccgcagcggc gtgatgcttc cgagcacgtg cggggagaga
     5461 gcggagggat tcctctccag ccccgcgcgt gctcgtcgtc ccccgtcagg acgggcgggg
     5521 ggaaggcggc gcgcgccttc ccgtatctgt gtctgtgtgc gctcgcggag cgcgggcgcg
     5581 cgcgggtctc gctgcggcgc gtaggcgccc gcgcgagtcg tcgcatgagt ctggtcgtcc
     5641 ggcgtcttcg tgcgcgcgtc gagagcgcgg cggaggtcgg gcgcgtactg gcggcgcgcg
     5701 aggttctgcg cgcggccggc gaagcgagca gtcgcgaaga aggaagcggc gagcaggaga
     5761 gcgcagagga tggtgacgag gcgaatgagc ttcgacatga tgcttgaaaa ccccgcggcg
     5821 tgtgccgcgc taatgtgtgc ggcggaggtg agacggcgtg cgcctaaagc ctatggtcgt
     5881 gtcgcacgcc gcctcaccgc cgccgcgccg tggccgcgaa gcccctcgac gcggccacag
     5941 tgctaagtcg cacgaatcac ggctcatctg acttcaccgg ctccctgttc ctcgcggggc
     6001 gagcgggacg cgcggagtcg cgggaaggcg gcgccgggcg ctcctgtcgt tgcggcggcg
     6061 gggcggcggg gcgctcatta ctcggtggcg gcgcactgtc gcgttgcgga ggcgtctcct
     6121 gtcgcggcgc cggctcgtgt cgcggtgcgg gccgctcctc cctctgcggc ggcggatcgt
     6181 gacgcaccgg gggagacaga cgttcctcct cccttggcct cacacgctcg cgcggctccg
     6241 ggcgctcgtc ggcgcgcggc cgggacgcgg gacgctcgtc gcttggttgc tctctgtcaa
     6301 cttcaggacg cccgacgcgc gacggacgcg cgggccgtgc cgcgtcccct tcatcatcgc
     6361 gttcacgctc tgcgggtcgg gtgcgttcag tgggtacaga cgtgcccggc gacgggaacg
     6421 aacgctcgga ctcgcccggc gtgcgcggcc gacgaacgga cggcgcagcg ccctcggcgg
     6481 gcaagccgcg ctcagtgggc gacacacgcg cgggccgcgc ggggcgggcc acggcgcccg
     6541 tgccgcggtc atcatcgcgg gcgtcggcgc cgtcggatgt cggggagggg aatctcaatt
     6601 cgcgcccgcc gagcacgcgc gtgcgacgca actcttcgtc gagcgggacg ccgggcgtgc
     6661 gtgaggcggc gcctgtatcg cgctgcggaa tgtcgcgcgg gggcgtgacc ggcgcgctcc
     6721 ggttgattct cagtccctcg cggttgccgg ggcgcgcccc agctcctgaa tcggccgcga
     6781 caccggcccc cgtgcgaact tcgccgcgtg ggccgacgcg tgcgggggtg gcgaaggttg
     6841 aagttcggcc cacgtcggcg ggcgtgatgg gcaggcgacc gcgcaccggc tcgcccgtca
     6901 ccgtgcggcg cgcgatgtcg gcggcggcgg cgcgcccgcg cagggagtcg gctccgaagt
     6961 cacgcgccgg caccgccgtc accccgcgca ggaggagcgg gttgacacgc tcaaggttgg
     7021 cggcatcgcg tgcgcgcagc ggcgcgaggc ggtcggggcg tcgctgcccc cagcggccgc
     7081 gcgggtcgtg ctgtccgtgc cggagcggat accacgcgac gtgctcgcca taagaggtcg
     7141 ggatgtagac gaaggcgaca agcgccggcc gccaccacgc gcgacggtat tcgtagccgt
     7201 agccgcgcgg cgcccagcac cagttgttgt tgtagacgac ccagcgcccg tagtggtaag
     7261 gggcccagcc ccattcctca tccgccaccc acgtccaacc gtagggatga acccagcgcc
     7321 agtggccgta acggtaaggc gcccagttgt gatagtggtt gacgaccgtc acgtgcggac
     7381 gccacaccca gccatactgc gacgtgtgat gccagtcgcc ataggagtcc agttcctcag
     7441 cgccccacac gtcgcggtcg tagtagcgtt cgcgctgctc gtagcgcaga cgcgaggcga
     7501 ggttgcgctc gcgctcgtcg ttccatgtgt cccactcgtc gaaggctgag gccgtggcca
     7561 actcccagtc gccctcgccg tcctcgatgt agagacgcgc cgagcggttg ttgcggagag
     7621 tgaagccgga cgactcggaa tagacgcgcg cccggccctc gtcgcgcacg gtgacgcgga
     7681 cgccgccgtc gcgcgccacg tcaacgcggt agaggccgcg ccgctcggcc gccacggtgg
     7741 tgcgcggcgc gtccagctcg aaatactctt tgtccttgtc gaagcgcgcg agccgcagcg
     7801 tcgcggcgcc ttcagagaga ctgacggcga tgccctcctc gcgcaacgtg acgacgcgca
     7861 gaacggagtc cgcgccgacg cggacgaagt tgtgcgcgtc gatctgaatt tccagacgcg
     7921 agtcggggcc ggtggcgagc gtgtcgccct cgacgagcgg gaggttgagt ttggcgcgct
     7981 cccactcgac gttgcccgcg cgccgcaaac tcacctcgcc gcgcatgaga ctcacgcgca
     8041 tcacgcgcgc gtgctcctcg tactcgtttt cgtcaacgtc gtccgcggcc gaagcgcgcg
     8101 cggtgaatgt gaatgcggtt gcgaggaaga gaagcgggag tagtaaggac ttgagtgaac
     8161 gcatgggaca gtacgctcct taacttttcg actcgaagga aagcgggacg tgtgcgtgcg
     8221 ggagacgcac ggagggtaaa gctcggctac gggagcgaag cacttgcgag gcgctttatc
     8281 cagacgcatg cgccgagtgc aaagcgtgtg cccgtgcgcg aatcgcgagg aaaaatagac
     8341 gatgcgcgtg tgattcagaa gagacggacg gcgaggcgtc gagcagaccc atgcgagatg
     8401 gtgcaggccg tcgcactatt tcgtatgcgt cggctttgtg aaagtacagt accgcgagcg
     8461 gaagcctcgc gggtacgcta acgatgatag gcccgcctcg cgtatccaga cccactcgct
     8521 accgctcgcg gtactgtacc cgatgcgtga gccgaaatgt ttacagttaa tttacacgcg
     8581 gcggcggcgc gcgggcgtag aatacatcag acgattttgg aacctcgcga ggctttggag
     8641 cacatagtaa gacgagactc gggcggcgag tccgtcggcg gcccctaaag aaaagtcaac
     8701 cgccagttgc ccgaagacgt taaagctaca tgcggtgatg gtgattgaat ggcacgcgcg
     8761 gccgaagaaa tgtctgccac aaagtcagac ggggcggtgt tcacacaata gatgacaggt
     8821 tgtgagacgg ccgccggtca ggccctttcc gcacatgaaa ccacaatggc cgcaggcttt
     8881 ttcgagattt cgtcgcccat agacaaggga agaggtagtg agatgaaaca cagaacggtc
     8941 actgtattca tcgtcgttct ggcggccctg gcggccgtcc cacaggcttt tgaacaattc
     9001 tcctcgctca aggacgcggc cggcaaccgg ctgaccgcgg gtgtctggaa cgttttcctg
     9061 agtctccacg ggcagaaggt cgcgggcgcg ccgcgcgcgc agcaactccc gcagacggca
     9121 ctcctcgtcc gcgccgccgc gccggaaggc cccacgcaga tcgcgaggag agaggcgcgt
     9181 ccggcaaagt ctttgacggc atcgtcgtcc ccgctgagcg ccacgcatct gcgcgccgaa
     9241 gaggcgtt
//
LOCUS       DASVES010000294        19529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_12306, whole genome shotgun sequence.
ACCESSION   DASVES010000294 DASVES010000000
VERSION     DASVES010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19529
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12306"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="metG"
                     /locus_tag="VF240_11850"
     CDS             <1..1179
                     /gene="metG"
                     /locus_tag="VF240_11850"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEX7175944.1"
                     /translation="IDPVALHRHVGNDAVRYFLLREMSFGQDCNVGYESIIDRANSDL
                     ASGLGNLVSRTLTMIKRYCDGAVPAGEIPEEQRLSAKRAGVDTDAQSLATVLEHARNQ
                     FVAGFDELAYHRALESVWGAISRVDKLISESKPWELAKDEKQRPALGAVLYRAAESLR
                     WLAALLYPVMPESAASIWRQLGQTTDITKTNPTELTWGGLREGTLVGDVAPLFPRLDK
                     KKIMDTIEEENAQKNAPAETRATQAAVPGSEAAAETKPAPPAPGATEADAVPPAAKEK
                     AADAGGGLITIDDFVKVELRVAQVLTAERVPKADRLLRLTVDVGEESPRQILAGIALH
                     YEPESLLGRKIIVVSNLQPRKLRGLESQGMLLAASAGDEGKPILATFAEDVPNGTKLK
                     "
     gene            1274..2074
                     /locus_tag="VF240_11855"
     CDS             1274..2074
                     /locus_tag="VF240_11855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7175945.1"
                     /translation="MFVDSHAHIDGEEFAEDRDEVVARAREAGVRAILNVGTGDPHAG
                     SLERAVETAERYDGVYAAVGVHPHDARLFDTAAEELLLRLVKGNAKVIAWGEIGLDFH
                     YDNSPREIQREVFARQLRLARECELPVVIHSRDADEETIDVLQDEWAGAGLAGILHCF
                     GGGQSMAERALELGFMISFAGNVTFKKADALREVARQVPLERLLVETDCPFLTPAPHR
                     GKRNEPARVVDTARFLAELRGESLEELGRVTSANFERLFGLELGAAPV"
     gene            2230..2727
                     /locus_tag="VF240_11860"
     CDS             2230..2727
                     /locus_tag="VF240_11860"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX7175946.1"
                     /translation="MAQQNSFDIASKVDRSEIVNAIDQTMKEVRQRFDFKGSAAEVQL
                     EEKDLLLSAEDETKLRNMNDILQQKLVRRKVPLKALSYGKVEPAAGGTVRQRAAIQQG
                     IPTEKAKEVVKFIKDTKAKVQASIQGDVVRVTGRDRDTLQDVIANLKGKDFGIHMDFI
                     NYRSN"
     gene            2849..3868
                     /locus_tag="VF240_11865"
     CDS             2849..3868
                     /locus_tag="VF240_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7175947.1"
                     /translation="MQTFGNTAAVEDAQLTARRVFSLVAPELALVEEEFERQARSNVQ
                     VVSYIGDYLRASGGKRVRPALTLLSTLAAGGDASRPNVVRLATVMEMLHTATLVHDDI
                     IDNAELRRNRPSVNSRFGNQTAVLMGDWLYMSAFETSLQERSLAVLDILTAVTRKMTE
                     GELLQLMLLGSVDVTEEQYFDVLRRKTAYLFSACCEIGSMLGGAGESERRALRDYGLH
                     LGTAFQLVDDLLDFTGTDEAMGKASGVDLLEGKLTLPLIYLLEDDATARAAVKTVIEE
                     GAFEKINRAMLIDAAEKSGALRRARSRAAEYADGARKALDTLKPSKYTDALRSIPTFI
                     LEREM"
     gene            3874..4470
                     /locus_tag="VF240_11870"
     CDS             3874..4470
                     /locus_tag="VF240_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175948.1"
                     /translation="MGSENLLATLEQSLRQARVTRSRIASRLADIEAEAERSRTELSE
                     MDALVSQTEAAMFRIMSGMFTAQTNASPSDAEIEAALRRDAVRAAAQQQQTQPSPVAS
                     VPRPAMPAVRSNAEPMNDRFSDRTIPQATAMLLREAGGPLHVNEIYNRLIEGNFQFTG
                     HHPTISIAVSLNRNQRFRKVAPGTFDLVIRDASQTSTG"
     gene            4581..5603
                     /locus_tag="VF240_11875"
     CDS             4581..5603
                     /locus_tag="VF240_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX7175949.1"
                     /translation="MRAMILAAGYGTRLWPLTIDRAKPALPFMGRPLVGYVAEYLARH
                     GAREVVVNLHHRPESVRASLGDGTRFGVRLHYVEEHDILGTSGALDNARALLEGDTFV
                     VINGKLATDIDLGAALETHRRTNALATLVLRQNPKRERFSTVETKDGMVMGFGPAPKP
                     ASASDNVFDNGGAGEIETVEDVPLMFTGIQILEPRIFDFIPRGVFSHSTTDVYPKAIE
                     QGERIAAHVAPGMWYELSTIQRYLDISVELMGREGRDVEIGEGSVVAAGAAVRESVLW
                     EGVRVEEGARVRRAVLGAGVHVGSGEVFEDVAVVRAGIVREEERPEKALRGEVRGANF
                     VVSLPR"
     gene            5720..6778
                     /locus_tag="VF240_11880"
     CDS             5720..6778
                     /locus_tag="VF240_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7175950.1"
                     /translation="MNHPTTITTHAFETSARDRLAAYIARAGAKPDRIIALTPDASTR
                     EYFRVPWKRSTAVAAVYPEPFDPDIHPYLDVTRLFHESGLPVPSILDVDALSGIIVQE
                     DLGDQQLRRVFEAASEDEREEYVERAISLIADIQGATAAAVERNSISSRLAFDEAKLS
                     WELDFFMEHYFKSLRREQLRHAEEAELRAEMNDVAAELSARPRVLCHRDFHSSNLMID
                     RRKRLRVIDHQDARMGPASYDLVSLLLDRQTCVPSLAEVRERRLFFLDERRERGLEMI
                     DPDEFAREFRLMTVQRCLKATGTFSYQSAVAGRAEVYEQFIEPMLLIVLQAAEWLGRF
                     PILQAALRARTGGAAAAN"
     gene            6857..8188
                     /gene="asnS"
                     /locus_tag="VF240_11885"
     CDS             6857..8188
                     /gene="asnS"
                     /locus_tag="VF240_11885"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX7175951.1"
                     /translation="MSTIVGAKQVYINRLSEHVGEEVTLRGWLYNLRSSGKLIFPQLR
                     DGTGVVQCVVFKKSVTPEVWEAFEGAGQESPLIIRGSVRADERAPGGYEVDVSEAQVL
                     QKVEGYPISPKEHGVEFLMDHRHLWLRSRRQHAILKVRHTVVRAVRDFLDTDGFTLCD
                     TPILTPAACEGTTTLFEVDYFDDEKAYLSQSGQLYNEATAAAFGKVYCFGPTFRAERS
                     KTRRHLTEFWMVEPEMAYATLDDVLELAERFLSSIAARVLDERAEELKVLERDTSKLE
                     SIVPPFPRLTYDEAVKILHEGHERGELETRFEWGGDLGSPDETFISSRFDRPVMVHRY
                     PAAVKAFYMKRDPERDDLALGVDVLAPEGYGEVIGGGERATSLEFLEEQLAAHKLPRE
                     AFEWYLDLRRYGSVPHAGFGMGIERCVAWMCGIEHIRETIPFPRMLYRLRP"
     gene            8287..8826
                     /locus_tag="VF240_11890"
     CDS             8287..8826
                     /locus_tag="VF240_11890"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX7175952.1"
                     /translation="MERIVTIFGGSKCGEDSEEYRHALRVGQLLAEQGFTICTGGYLG
                     VMEAASRGAHERGGHVLGIVMNQFKAEPNRYLTDKVATEHFYERLQNLITRSVGFIAV
                     RGGMGTVTEMSLVWNKLQTQVIESRPLVLLGKDWPPIIDAFRRHLAVSERDVQLLDFA
                     ETPEEAVGIVAEKSKGVNP"
     gene            8823..9728
                     /locus_tag="VF240_11895"
     CDS             8823..9728
                     /locus_tag="VF240_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX7175953.1"
                     /translation="MNCPNCGATLMSGAQFCGACGTRLSDAPDATAAPPPSPPPAQQP
                     PVSGPPPGHGIHIDEDARGQGRGYTYEVLHQPSFALAVLKLQAEQSIQAEAGAMVSMS
                     ANVELQSQMKGGIFGAIKRAAGGESAFVSTFTARGGAGEVTLAPGGPGDIAAIEMGNQ
                     HFFVQSSSYLAGDVGLTIDTRWGGAKSFFGGEGLFVLQVTGTGVLLVSSFGAIHRKRL
                     EPGERYVVDTGHLVAWEGSTQYTLRKAAAGFFRSLVSGEGVVAEFTGPGEILIQTRNL
                     AAFAGMMKPFFPTQGGGGSGFTFGQ"
     gene            9744..10712
                     /gene="rocF"
                     /locus_tag="VF240_11900"
     CDS             9744..10712
                     /gene="rocF"
                     /locus_tag="VF240_11900"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEX7175954.1"
                     /translation="MSETLLEQTKSAAAGRPAGAGRRVSIIGVPCGFGASIAGVDLGP
                     AAMRVARLRQRIAQLGYEVRDLGDMRLESPDTQPPPQEKTKYLKEITAACTQLAADVK
                     ASLADGELPVVLGGDHSIAIGSVAGVASFYREQEKPVGLIWFDAHADMNTPESSPSGN
                     IHGMPLSALLGFGAPELTGIEGFSPKLDPRYCAHIGARDIDPGERELIRRLGLRFFTM
                     REIDERGMGTCVDEAIHIAAQAPAGYHVTLDVDALDPGDAPGSGTVVRGGLTYREAHL
                     AMEKIAEAGGMLSLEVVEINTALDVNNKTAELGVELILSALGKTIL"
     gene            10778..11911
                     /locus_tag="VF240_11905"
     CDS             10778..11911
                     /locus_tag="VF240_11905"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX7175955.1"
                     /translation="MAKKIKVGVIFGGRSGEHEVSLRSAKSVIEAIDRQKYEVVPLAI
                     SKEGRWLSPSESAGLLPPSASSRLPERVGEPDRALTILADPSRKGLVSLESGGDGASP
                     EKVDVIFPVLHGTYGEDGTLQGLLEMAGVPYVGCGVLASSCGMDKVTMKTLFRQAELP
                     ICRYTWFLRSAWERDREAIARRVARETGYPCFVKPANLGSSVGISRATDRRTLHDAIA
                     LAARYDRRIIVEEGLDAREIECAVIGNDEPEASLPGEYRVLEKGSEFLDYTEKYSSTG
                     HVDFVVPAPLPKRLVTLIRQMSIRAYKALDCSGLARVDFFLRRDTKELLINELNTIPG
                     LTDVSGYPKMWEASGLPFEKMIDRLIELAFERYGDKSRNETSL"
     gene            complement(12052..13002)
                     /locus_tag="VF240_11910"
     CDS             complement(12052..13002)
                     /locus_tag="VF240_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7175956.1"
                     /translation="MTKHSIARIDTADATHRLTLRVSLTVIILLFASIPCSAQSEQDD
                     DVVRVESDLVVLNVTVTDKQGKYVRRLPLKEFKVFEDGIEQKISTFALEENPFAAAIL
                     IDTSGSMEGRISLARAAAIRFLDRLRPDDVVTIFNFDSKVEQVQDFSNSRDLPSLAYD
                     LRAEGQTKLHDAVVHASQALSKRPETRRAILVLSDGIDTKSSASLDKALNAALAANVT
                     IYTVNMTDPGLPSTQRQVLAGALKKLSEKSGGRYVASPGGRSLAEALEDIISELSNQY
                     TLGYRPKNRARDGRWRAIEITLTRQDLNSRTRAGYRAPKS"
     gene            complement(12999..13598)
                     /locus_tag="VF240_11915"
     CDS             complement(12999..13598)
                     /locus_tag="VF240_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175957.1"
                     /translation="MTRFTSFGLRSEVMTNLISSKLLFQKLLFTGILIFGLTSDVRAQ
                     SSSDNSSPLIPNSIKEAPSPVGPEAEMLHRAAIRRQEEAHLEMVERAKENTRLGFQLA
                     ASVGKVVSPHADEIKKLDRMEKLARKIRGDSGGSEDDNALQNLPGDVAASIRLLAETS
                     ENLLKEVEKTSRHVISATVIKRSNEVIHLVRHVKSFVRR"
     gene            complement(13760..14341)
                     /locus_tag="VF240_11920"
     CDS             complement(13760..14341)
                     /locus_tag="VF240_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175958.1"
                     /translation="MTNPRPSRTSTPYQLFLLPAVLLLIISIISCGNSESPGPIGSTS
                     GLKSNTRVSKPPAVNVFENEPLLKGSQAVIGGTIQNVSNQKLTDLIVEVELTGRVGGG
                     RTLKEMQVTPASISPGETGSYSLTISNREWAASKIVRLRSLIQAEEVAFNTLPGAKRP
                     PERTPEGKTIIVQKPKPKGEEFINSPDDADPIR"
     gene            complement(14421..15047)
                     /locus_tag="VF240_11925"
     CDS             complement(14421..15047)
                     /locus_tag="VF240_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175959.1"
                     /translation="MFVMLSFAAVAQGQEKGVDQNNERIRDNSTNRAPAVNGGKVDTG
                     AGRGVDFGKGRTPTPPPLSNPYRFSSPNDVVQKALLELLQERKLILDENVSKPTEGIL
                     ITQPFTFIRGVVFASGGLSQVAEVPASDTRGWTRGRYTLTVEVFPIDASSTDVSFNAK
                     IEGRSEGITGAEWVTLRSTGAVEQEFLIRLIQNLTGAPPTGYEPIDEP"
     gene            complement(15215..16288)
                     /gene="mltG"
                     /locus_tag="VF240_11930"
     CDS             complement(15215..16288)
                     /gene="mltG"
                     /locus_tag="VF240_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7175960.1"
                     /translation="MRAFIIILTLLILSAALFSAWAYKELKTAREHTHANQYLEIKRG
                     TNPEEVINTLTNAGVIRRGWLFKLYVRLSGSGPRLKAGEYRFASPIAPVEVLRKLEEG
                     EQRLSRLTVIEGWTRWDIAAQMARLPELKLQSPEEALALMNEVSLIADIDPIASNLEG
                     YLYPETYSYPPDTSARDVIALMVSRFRQVWEGRFSEVARSSGRSPREIVTVASLVETE
                     AKLNEERPLVASVIYNRLRLNMALGIDSTVIYASKLAGKWRNDGKVYQSDLDRDSPYN
                     TRKVVGLPPGPLASPGALSIEAALSPAATDYLYYVREPSRNDGAHNFYATESDFQRGV
                     QRLREWERTRDAQANTNTAATPQ"
     gene            complement(16331..16735)
                     /gene="ruvX"
                     /locus_tag="VF240_11935"
     CDS             complement(16331..16735)
                     /gene="ruvX"
                     /locus_tag="VF240_11935"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7175961.1"
                     /translation="MLAIDPGRKRVGLAVSDELRLTARPLSPLRRSNWKDLLQSLSDV
                     VRRFDVKTVVIGLPLLLEGAEGDAAEEARRFGRNLQLSLNLPVYFQDERLTSREAEET
                     LRAEGYSESAIAGRIDGEAARLILLDFISHSS"
     gene            16974..18263
                     /locus_tag="VF240_11940"
     CDS             16974..18263
                     /locus_tag="VF240_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7175962.1"
                     /translation="MSFRGKFALVVFSATLALYAVAGGLLSGTAQQQNDPGAQLRIFE
                     SVLHHIQTDYVDEPDLGKVRAGALRGLAYGLDPYSTYLTVDQVRDFRAGVQNTQVGIG
                     AELSQVASYLYVIAPVKGGPADQAGLLAGDIVEYIDGKATRDISLYDARQLLSGAPGT
                     EAKLRVLRAGTRPFALSIKRASVAVPAAESRMEAGQVGVIRVNSLDAGEAADVKARAQ
                     ELIKQGAQKILLDLRSVAGGTLAEGVKVANLFVREGVIAQTVKRGNKTDKVFNADPKE
                     AIFDGPAAVIIDRGTAGAAEIIASAFVERKRGEVVGEKSFGAGAEQQLFTLRNGDGLL
                     LTTIKWASSNGKPFLPEGVKPSVEVKRPELAEAIDPDELTGNDDDPIAQPNTQGDRQN
                     VAPEPAAPKQQPEDVQMKKALELLREKAKPASPQKAG"
     gene            complement(18326..>19529)
                     /locus_tag="VF240_11945"
     CDS             complement(18326..>19529)
                     /locus_tag="VF240_11945"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX7175963.1"
                     /translation="AIRVGAPVIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGVVP
                     QISCIMGPCAGGAVYSPAITDFNVMVKNTSYMFITGPDVIKTVTHEEVSKEELGGADT
                     HNRVSGVAHFSADSDEHALLLVRELLSFIPSNNMEDAPRLPCSDSPERADADLDTIVP
                     EASNQPYDIRDVIHRVVDEGYFFEVHEHFAQNIVVGFARLDGRSVGIVANQPAFLAGV
                     LDIDASVKGARFVRFCDCFNIPLVVFEDVPGFLPGVRQEHGGIIRHGAKLLYAFAEAT
                     VPKVTVITRKAYGGAYCVMASKHIRTDVNFAWPTAEIAVMGAEGAVGVLYRREIGEAK
                     DQEAARRARISEFEEKFANPYKAAERGFIDEIIEPSQTRQKLIRALSLIENKRDTNPP
                     KKHGNIPL"
BASE COUNT         3811 a         5915 c         6100 g         3703 t
ORIGIN      
        1 atcgaccccg tcgccctgca ccggcacgtc ggcaacgacg ccgtgcgcta ctttctgctg
       61 cgcgagatgt ccttcggtca ggattgcaac gtcggctacg agtcgatcat cgaccgcgcc
      121 aacagcgacc tcgccagcgg cctcggcaac ctcgtcagcc gcacgctaac gatgatcaaa
      181 cgctactgcg acggcgctgt gcccgccggc gaaattcccg aggagcagcg tctctcggcc
      241 aagcgcgccg gcgtggacac ggacgcgcag tcgctcgcca ccgtccttga gcacgcacgc
      301 aaccagttcg ttgcgggctt cgacgagctg gcctatcacc gcgctctgga gtcggtctgg
      361 ggcgccatct cgcgcgtcga taaactcatc tcggaatcga agccgtggga gctggcgaag
      421 gatgagaagc aacggccggc gctcggcgcg gtgctctatc gcgcggccga gtcgctccgc
      481 tggctcgccg cgctgctcta tcccgtcatg cccgagtcgg ccgcaagcat ctggcgacaa
      541 ctcgggcaga cgacggacat cacgaagacc aatccgacgg aactcacatg gggcggcttg
      601 cgcgaaggga cactggtcgg cgacgtagcc ccgctcttcc cgcgactcga caagaaaaaa
      661 atcatggaca ccatcgaaga agagaacgca cagaagaacg ctccggccga aacccgcgcc
      721 acgcaggccg ccgtgcccgg ttccgaggca gccgctgaaa ccaagcccgc ccctcccgca
      781 cccggcgcaa ccgaagccga cgccgtcccc ccggcggcga aggagaaggc ggcggacgcc
      841 ggcggcggcc tcatcaccat tgacgacttc gtcaaagtgg aactgcgcgt cgcacaggtg
      901 ttgacggccg agcgcgtgcc gaaggccgac agactcctgc gtctgacagt ggacgttggc
      961 gaagagtcgc cccggcagat actcgccggc atcgccctgc actacgagcc ggaaagtctg
     1021 ctcggacgca agatcatcgt cgtctccaac cttcagccgc gcaagctccg cggcctcgaa
     1081 tcgcagggca tgctcctcgc cgcctcggcc ggcgacgaag gcaagcccat cctcgccacc
     1141 ttcgccgaag acgtgcccaa cggcacgaag ctgaaatgaa ggagtcagga gtcagaagtc
     1201 aggagtcaga ataaaacccg aagaaggagc attcattctg acttctgact tctgacttct
     1261 gactcctgtt tttatgttcg ttgattcaca cgcgcacatc gacggggagg agttcgcgga
     1321 agaccgcgac gaggtggtgg cgcgggcgcg cgaggccggc gtgcgggcca tcctcaacgt
     1381 cgggacgggc gacccgcacg ccggcagtct tgagcgggcg gttgagacag ccgagcgtta
     1441 cgatggagtc tacgcggctg taggcgttca tccacacgac gcgcgtctgt tcgatacagc
     1501 ggccgaagag ttattgctga ggctggttaa agggaacgcg aaagttatcg cgtggggtga
     1561 gatcgggtta gactttcatt acgacaactc gccgcgcgag attcagcgcg aagtcttcgc
     1621 cagacaactg cggctcgcgc gcgagtgcga gctgcctgtc gtcatccatt cgcgcgacgc
     1681 ggacgaggag accattgatg ttctacagga tgagtgggcg ggggccggac tcgcgggcat
     1741 cctccactgc ttcggcggtg gacagtcgat ggcggagcgg gcgctggaac tcgggttcat
     1801 gatttcgttc gccgggaacg tgactttcaa gaaggccgac gctttgcgcg aggtggcgcg
     1861 gcaggttccg cttgaacgtc tcctcgtcga gaccgactgc ccgttcctca cgccggcgcc
     1921 tcaccgcggt aagagaaacg aaccggcgcg cgtcgtggac acggcgcgtt tcctggcgga
     1981 gttgcgcggc gaaagtttgg aggaactggg gcgggtcact tcggccaact tcgagcgcct
     2041 cttcgggctg gagcttgggg ctgcgccggt ctgagcgggc gccgacggcc gcggagtttt
     2101 ttttaaagtc cggccccgtc ttcttttccg gcaatgaggg ataatgttat aaactgtgcg
     2161 gcggggaaga aagccctccg gcacggggcg gaattcagcg tgaccccgcg agcatcccca
     2221 gtcgcacaca tggctcaaca aaactcattc gacatcgctt ccaaagttga ccgcagcgag
     2281 atcgtcaacg ccatcgacca gacgatgaag gaagtccgac agcggttcga cttcaagggc
     2341 agcgccgccg aggtgcagct cgaagagaag gaccttctgc tttcggccga ggacgagacg
     2401 aagctgcgga acatgaacga catcctccag cagaagctcg tgcgccgcaa ggtgcccctc
     2461 aaggcgctct cctacggcaa agtggagccg gccgccggcg gcaccgtccg tcagcgcgcc
     2521 gccatccagc agggcatccc cacggagaag gccaaagagg tcgtcaagtt catcaaggat
     2581 acaaaggcca aggtgcaagc ctcgattcag ggcgacgtgg tgcgcgtgac gggccgcgac
     2641 cgcgacacgc tccaggacgt gatcgccaat ctcaagggca aagacttcgg tatccacatg
     2701 gattttatca actacaggtc gaactgaaag aaaagccgtg aatcgtcaat cgtcaatcga
     2761 ttgacttgca ttcatcgccc gattcaccgc tcaggctgaa cacctctcaa ccacgattca
     2821 cgattcacga ttcacggctt tttattctat gcagactttc ggaaacacgg cggcggttga
     2881 ggatgcgcaa ctgacggcgc ggcgcgtctt cagtcttgtc gccccggaac tcgccttggt
     2941 ggaggaggag ttcgagcggc aagctcgctc gaacgttcag gtggtctcct acatcggcga
     3001 ctacctgcgc gcctcgggcg gcaagcgcgt ccgtccggcc ctgacgctgc tctcgacatt
     3061 ggcggcgggc ggcgacgcct cgcgcccgaa cgtcgtccgc ctggcgaccg tgatggagat
     3121 gcttcacacg gccacgctcg tccacgacga catcatcgac aacgccgagc tgcgccgcaa
     3181 ccgcccttcg gtcaacagcc gcttcggcaa tcagacggcc gtgctgatgg gcgactggct
     3241 ctacatgtcg gcctttgaga ccagcttgca ggaacgctcc ctcgccgtcc tcgacatcct
     3301 gacggccgtg acacgcaaga tgaccgaggg cgagcttctc cagctcatgc tcctcggcag
     3361 cgtggacgtg acggaggagc agtacttcga cgtgctgcga cgcaagacgg cttacctgtt
     3421 cagcgcctgt tgcgagatcg gctcgatgct cggcggggcg ggggagtcgg agcggcgcgc
     3481 tctgcgcgac tacggactcc acctcggcac ggcgttccaa ctcgtagacg atctgctcga
     3541 cttcacgggg acggatgagg ctatggggaa agcgtccggc gtcgatctgc tcgaaggcaa
     3601 gttgacgctg ccgctgattt acctgctcga agacgacgcg actgcccgcg ccgccgtgaa
     3661 gaccgtcatc gaagaagggg cttttgaaaa gatcaaccgg gcgatgctca tcgacgccgc
     3721 cgagaagtcg ggcgcactgc gtcgcgctcg ctcccgcgcg gccgagtacg ccgacggcgc
     3781 gaggaaagcc ttggacacac tcaaaccgtc gaagtacacc gacgcgttgc gctcgattcc
     3841 gacgttcatc ctcgaacgcg agatgtgaca gacatgggca gcgaaaacct actcgccaca
     3901 ctggaacaaa gcctgcggca agcgcgcgtt acccgctcgc gcatcgcctc gcgtctcgcc
     3961 gacatcgaag ccgaggccga aaggtcgcgc acggagctgt cggagatgga cgccctcgtc
     4021 agtcagaccg aggccgcgat gttccgcatc atgtccggga tgttcaccgc gcagacgaat
     4081 gcctctcctt cggacgcgga gatcgaggcg gcgctcagac gcgacgccgt ccgggccgcc
     4141 gcgcagcagc agcagacgca gccatcgccg gtcgccagcg tcccgcgccc cgcgatgccg
     4201 gccgttcggt cgaacgccga gccgatgaac gaccgcttct ccgaccgcac gatcccgcag
     4261 gccacggcga tgctcctgcg cgaagccggg gggccgctcc acgtcaacga gatttacaac
     4321 cgtctcatcg agggcaattt tcaattcacc ggccatcacc ccaccatcag catcgccgtc
     4381 tccctcaatc gcaatcaacg cttccgcaag gtcgcccccg gcaccttcga cctcgtcatc
     4441 cgcgacgcct cccaaacttc aaccggctga gcgcattctt ctccgtcgcc cttcgcatta
     4501 aaaatttcga taagctcatg gcgatttgac tctcagagtc tcttcggcga acctctatca
     4561 aaacttcgga gggaagacta atgcgcgcga tgattctcgc cgccggctat gggacgcgac
     4621 tctggccgct caccatcgac cgtgcgaagc cggcgctccc tttcatgggt cggccgctcg
     4681 tcggctatgt ggccgaatac ctcgcgcgtc acggcgcccg agaggtcgtc gtcaatctgc
     4741 accaccgccc cgagtcggtg cgcgcgtctc tgggcgacgg cacacgcttc ggcgtgcgtc
     4801 tccactacgt cgaagagcac gacattctcg gcaccagcgg cgctctcgac aacgcccgcg
     4861 ccttgctcga aggcgacacc ttcgtcgtca tcaacgggaa actcgccacc gatatcgacc
     4921 tcggcgcggc acttgaaact caccgccgga cgaacgcgct cgccacactt gtcctgcgcc
     4981 aaaacccgaa gcgcgaacgc ttcagcacag tggagacaaa ggacggtatg gtcatgggct
     5041 tcggccccgc gcccaagccg gcaagcgcat ccgataacgt ctttgataac ggtggggccg
     5101 gcgagattga aactgtcgaa gatgtccctt tgatgttcac tggcatccag attcttgagc
     5161 cgcggatttt cgatttcatt ccccgcgggg tcttctccca ttcgacgacg gacgtttatc
     5221 caaaagccat cgagcaggga gaacgcatcg ccgcgcacgt cgcgccgggg atgtggtacg
     5281 agctttcaac gattcagcgc tacctcgaca tcagcgtcga actgatgggg agggaagggc
     5341 gcgacgtgga aatcggggag gggtctgtcg tcgcggcggg ggcggcggtg agggagtcag
     5401 tgctctggga gggagtcaga gtcgaggagg gtgcgcgggt gcgacgcgcg gtgttagggg
     5461 ccggcgttca tgtggggagc ggtgaagttt tcgaggacgt ggcggtcgtc cgcgcaggaa
     5521 tagtgcgcga ggaagagcgg cccgagaagg ccctgagggg agaggtgcgc ggggcaaatt
     5581 ttgtcgtgtc cttgccccgg tgatacaatc caccccggcc cctttttcag ccgtggcggt
     5641 ggcactatca acaatcttga ggggtagtgt ttctacagcg cgcgtcgagc gccgcgcgag
     5701 gctcgtgtcc caactccgca tgaaccatcc aacgacgatc accacccacg ccttcgagac
     5761 ttcagcgcgc gacaggctgg cggcatacat cgcccgcgcc ggggcgaagc cggatcgcat
     5821 catcgcgctg acgcccgacg cttccacgcg cgaatacttc cgcgtgccct ggaagcgttc
     5881 cacggccgtc gccgccgtct accccgaacc cttcgaccct gacatacacc cttacctcga
     5941 cgtgacgcgg ctcttccatg aatcggggct gccggtgccc tcgatcctcg acgttgacgc
     6001 gctcagcgga atcatcgttc aggaagattt gggcgaccag caactgcggc gcgtcttcga
     6061 ggccgcctcc gaggacgagc gcgaggagta cgtcgagcgg gccatcagcc tgatcgccga
     6121 cattcagggg gccacggccg cggccgtcga gcgcaactcc atctcgagcc ggcttgcctt
     6181 cgacgaggcg aagctctcgt gggagctgga cttcttcatg gagcattact tcaagagtct
     6241 ccggcgcgag caactcaggc acgccgagga ggccgagttg cgcgcggaga tgaacgacgt
     6301 ggcggccgag ttgtccgcgc gcccacgcgt gctctgccac cgcgacttcc actcctccaa
     6361 tctgatgatt gaccgccgca agcgcctccg cgtcatcgac catcaggacg cacgcatggg
     6421 gccggcaagc tatgacctcg tctcacttct cctcgaccgg cagacatgcg tcccctcgct
     6481 cgccgaggtg cgcgagcgca ggctcttttt tctcgacgag cggcgcgagc gcggtcttga
     6541 aatgatcgac ccggacgagt tcgcgcgcga gttccgcctg atgacggtgc agcgttgcct
     6601 gaaggcgacg gggacgttct cctaccagtc ggccgtagcg gggcgcgccg aggtctacga
     6661 acagttcatc gagccgatgc tcctgatcgt cctccaggcc gccgagtggc tgggccgctt
     6721 ccccatcctc caggcggcgt tgcgcgcgcg caccggcggc gccgccgccg cgaattagcc
     6781 ctgccggctt cgcgtagaga atcttttatt cggagtttcc tgaagtcatc gagcaaggag
     6841 tattgacgac aaagagatgt caaccatagt gggcgccaaa caggtctaca tcaatcggct
     6901 ctcggagcac gtcggcgagg aggttacgct ccgcggctgg ctctacaacc tgcgttcgag
     6961 cgggaagctc atcttcccgc aattgcgcga cggcacggga gtcgtccagt gcgtcgtctt
     7021 caaaaagtcg gtcacgcccg aggtgtggga agccttcgag ggcgccgggc aggagtcgcc
     7081 gctcatcatc cgtggaagcg tccgcgccga cgagcgggcg cccggcggct acgaggtgga
     7141 cgtgtccgag gcgcaggtct tgcagaaggt cgagggctat ccgatatcgc ctaaggaaca
     7201 cggcgtcgag ttcctgatgg atcaccggca tctctggctg cgctcgcgcc gccagcacgc
     7261 catcctcaag gtcaggcaca cggtcgtccg cgccgtgcgc gacttcctcg acacggacgg
     7321 cttcacgctc tgcgacacgc cgattctcac gcccgccgcc tgcgagggca cgaccacgct
     7381 tttcgaggtc gattacttcg acgacgagaa ggcgtacctg tcgcagtcgg gccagctcta
     7441 caacgaggcg acggccgcgg ccttcggcaa ggtctactgc ttcggcccga ctttccgcgc
     7501 cgaacgctcg aagacgcgcc gccacctcac cgagttttgg atggtcgagc cggagatggc
     7561 ctacgccacg ctcgacgacg tgttggagct ggccgaacgt ttcctctcct caatcgccgc
     7621 ccgcgtcctc gacgagcgcg cagaggagtt gaaggtgctg gagcgcgaca cgtcgaagct
     7681 ggagagcatc gtcccgccct tcccgcgtct cacctatgac gaggcggtga agattctgca
     7741 cgaggggcac gagcgcggcg aactcgaaac gcgcttcgag tggggcggcg acctcggctc
     7801 gcctgatgag actttcatct ccagccgctt cgaccgtccc gtgatggttc accgctaccc
     7861 cgcggccgtc aaagccttct acatgaagcg cgaccccgag cgcgacgatc tggcgctcgg
     7921 cgtggacgtg ctcgcgcccg aaggctacgg cgaggtcatc ggcggcggcg agcgggccac
     7981 ctcgctcgaa tttctggagg agcagctcgc ggctcacaag ctcccgcgcg aagccttcga
     8041 gtggtatctc gacctgcgac gttacggaag cgtcccgcac gccggtttcg gcatgggcat
     8101 cgagcgttgc gtggcctgga tgtgcggcat cgagcacatc cgcgagacga tccctttccc
     8161 gcgcatgctg tataggctca gaccataagg acgtgatacg tgatgagtga taagtgataa
     8221 gacaaggcgg tcaatcgtct cttatccctt tctcatcact tatcacttat cactcatcac
     8281 ttgtttatgg aacgcatcgt caccatcttc ggcggctcga aatgcggcga ggactcggaa
     8341 gagtatcgac acgcgctgcg ggtgggacaa ctgctcgccg agcagggttt cacaatttgc
     8401 acgggcggct atctgggcgt gatggaggcc gcgagccggg gcgcgcacga acgcggcggc
     8461 catgtccttg ggattgtgat gaatcaattc aaggccgagc ctaatcgcta cctgacggac
     8521 aaggtggcga cggagcattt ctacgagcgt ctgcaaaatc tgatcacacg ctcggtcggc
     8581 ttcatcgccg tgcgcggcgg gatgggcacg gtcaccgaga tgtcgctcgt ctggaacaaa
     8641 ctccagacgc aggtcatcga gtcgcgcccc ctcgtgctgc tcggtaaaga ctggccgccc
     8701 atcatcgacg cattccgccg acacctcgcc gtcagcgagc gcgatgttca gctcctcgac
     8761 ttcgccgaga cgccggagga ggccgtcggc atcgtcgccg agaaatccaa aggagtgaat
     8821 ccatgaactg ccctaactgc ggggccacac tgatgagcgg cgcacagttc tgcggggcct
     8881 gcggcacgcg cctgagcgac gcgcctgacg ctaccgccgc gccgcctccg tccccacctc
     8941 ccgcgcagca gccccccgtc tccggcccgc cgcccggcca cggcatacac atcgacgagg
     9001 acgcacgcgg ccaggggcgc ggctacactt acgaagtcct ccatcagcca tccttcgccc
     9061 tcgccgtgct gaagcttcag gccgagcagt cgatacaggc agaggccggc gcgatggttt
     9121 cgatgtcggc gaacgtcgaa ctccagtcgc agatgaaggg cgggatattc ggcgcgataa
     9181 agcgcgcggc gggtggcgag tcggccttcg tctcgacctt cacggcacgc ggcggggccg
     9241 gcgaagtgac gcttgcgccc ggcggccccg gcgacattgc agccatcgag atgggcaacc
     9301 agcacttctt cgtgcagtcg agttcatacc tcgcgggaga cgtgggactg accatcgaca
     9361 cgcgctgggg cggggccaag tcgttcttcg gcggcgaggg cctcttcgtc ctacaggtga
     9421 cgggcacggg ggtgctcctc gtctcatcct tcggcgccat ccaccgcaag cgtctggagc
     9481 cgggggagcg ttacgtcgtt gacaccggcc acctcgtcgc gtgggaaggc tcgacgcagt
     9541 acactctgag gaaggccgcg gcagggtttt tccgaagcct cgtgagcggt gagggtgtcg
     9601 tcgcggagtt caccgggccg ggcgaaattc tcatacagac gcgcaacctc gccgccttcg
     9661 cgggaatgat gaaacccttc ttcccgacgc agggcggggg cggaagtggt tttaccttcg
     9721 gccagtaaca aggggtgaca ggcatgagcg aaactcttct cgaacagacg aagtcagcgg
     9781 cggcaggacg ccccgccggc gccgggcgtc gcgtgagcat catcggcgtg ccgtgcggat
     9841 ttggcgcgag catcgctggc gtcgatctgg ggccggccgc gatgcgcgtc gcgcgtctcc
     9901 gacagcgcat cgcgcaactt ggctacgaag tccgcgacct cggcgacatg cgcctcgaaa
     9961 gccccgacac tcaaccccct ccgcaggaga aaaccaaata cctgaaagag atcacggccg
    10021 cctgcacgca actcgctgcg gatgtgaaag ccagcctcgc cgacggggag ctacccgttg
    10081 tcctcggcgg tgaccactcc atcgccatcg gttcggtggc cggcgtcgcc tccttctatc
    10141 gcgagcagga aaagccggtc ggtctcatct ggttcgacgc acacgctgac atgaacacgc
    10201 ccgagagtag cccgtcggga aacatccacg gcatgccgct ctccgcgctg ctcggcttcg
    10261 gcgcgcccga gctgaccggc atcgaaggct tctcccccaa actcgacccc cgctactgcg
    10321 cgcacatcgg cgcgcgcgac atcgaccccg gtgagcgcga actcatccgc cgcctcggcc
    10381 tgcgcttctt cacgatgcgc gagatcgacg agcgtgggat gggcacgtgt gtcgatgagg
    10441 ccatccacat cgccgcgcag gccccggcgg gctatcatgt gaccctcgac gtggacgcgc
    10501 tcgaccccgg cgacgcaccc ggctccggca ccgtcgtgcg gggcggcctc acctaccgcg
    10561 aggcgcatct cgcgatggag aagatcgccg aggcgggcgg gatgctctcg ctcgaagtag
    10621 tcgagatcaa tactgccctc gacgttaaca acaagacggc ggaactcggc gtcgaattaa
    10681 tcctgtcggc actgggtaaa acgatactct gaaggcccct atcgcgtccg cgactgaaga
    10741 tatgaaacgg ccggccgcgc catgcatcaa ccaaactatg gcgaaaaaga taaaggtggg
    10801 tgttatcttc ggcgggcgct cgggggagca cgaggtttct ctgcgctcgg cgaagtcggt
    10861 tatcgaggct attgaccgcc agaaatatga agtcgtcccg ctggccatct ccaaggaggg
    10921 gcgctggctc tctccgtcgg agtctgccgg cctcctgccc ccttcggcgt cgagtagact
    10981 ccctgaaagg gtgggggagc ctgaccgtgc gttgacgatt ctcgccgatc cgtcgcgcaa
    11041 aggtctggtc tcacttgaga gcggtggcga cggggcctcg ccggaaaaag ttgacgtgat
    11101 cttccccgtg cttcacggca cctatgggga ggacgggacg ctacagggac ttctggagat
    11161 ggccggcgtc ccatacgtcg gctgcggggt gctggcttcg agttgtggca tggacaaagt
    11221 gaccatgaag actttgttcc gccaagcaga acttcccatt tgccgctaca catggtttct
    11281 gcgctccgcc tgggagcgcg acagagaggc gatagcccgc cgcgtcgccc gcgagaccgg
    11341 ctatccatgc ttcgtcaaac ccgcaaatct cggctcctcg gtcggcatct cccgggccac
    11401 agaccggcgc acgctccacg acgcaatcgc cctcgcggct cgctacgaca gaagaataat
    11461 tgttgaggag ggattggacg cccgtgagat cgagtgtgcc gtaattggaa acgacgagcc
    11521 ggaggcgagc ctgccggggg agtacagggt attggaaaag gggtcggagt ttcttgatta
    11581 caccgagaaa tattcgagca ccggccacgt cgattttgtc gtcccggcgc ccctgccgaa
    11641 acgtctggtg acgctgatta gacagatgtc catacgcgcc tacaaagcgc tcgattgctc
    11701 cggcctcgca cgcgtggact tcttcctgcg ccgtgacaca aaagaactat tgatcaatga
    11761 gttaaatacg attccgggcc tgaccgatgt gtccggctat cctaaaatgt gggaagcatc
    11821 cggcctcccg ttcgagaaga tgatagatcg cttgatcgaa ctcgcctttg aaagatatgg
    11881 tgataaatcc cgcaacgaga caagtctata actcacaagt ctttgggcat gctctgttaa
    11941 gattgtttct cgcctgcttg atttccccta agccatcatt ccaaagttaa tgttgtactt
    12001 cgagcgagcc tgactaacgc ctgtcaggcg cgaggttaga ctaatacggc tttaggattt
    12061 tggggcacga tatccggcgc gggtgcgtga attcaaatct tggcgcgtga gtgtaatctc
    12121 gatggctcgc caacggccgt cgcgggcgcg gttcttcggg cgatatccga gggtgtattg
    12181 attactcaac tcgctgatga tgtcctcgag cgcctccgcc agagaccgcc caccggggct
    12241 ggccacgtag cgcccgccac tcttttccga aagcttcttc aaggctcctg ccagtacttg
    12301 cctctgcgtt gagggcaaac cggggtcggt catgttcact gtgtagatcg tgacattcgc
    12361 cgccagcgcg gcgttcaaag ctttgtcgag agaggcgctg cttttggtgt cgatgccgtc
    12421 tgagaggacg agtattgcgc ggcgggtctc cggccgcttt gagagcgcct gagaggcgtg
    12481 tacgacagcg tcgtgcagtt tggtttgccc ttcggcgcgg aggtcataag ccagggaggg
    12541 gaggtcgcgg ctgttggaaa aatcctgaac ctgttcgacc ttcgagtcga aattgaagat
    12601 cgtgacgaca tcgtcagggc gcagacggtc gaggaacctt atcgcggcgg cgcgtgcaag
    12661 cgagatgcgg ccctccatgc tgccggaagt gtctatcagg atggcggccg cgaacggatt
    12721 ctcctccaaa gcgaaggtgc tgatcttctg ctcgattccg tcctcaaaga ctttgaattc
    12781 cttaagggga agcctacgga cgtatttgcc ttgtttgtcc gtaacggtga cattgaggac
    12841 gacgaggtct gattcgacgc gcactacgtc gtcgtcctgt tcagactggg ccgaacaagg
    12901 tatggaggcg aaaagcagaa tgatgacggt cagggaaacg cggagcgtga ggcggtgtgt
    12961 tgcgtcggct gtatctatcc gagcaattga atgtttggtc atcgtctgac gaatgatttc
    13021 acatggcgga caagatggat gacctcatta gagcgtttaa ttaccgtcgc ggagatgaca
    13081 tggcgggaag tcttttcgac ttctttcaac agattctctg aagtctctgc taacaacctg
    13141 attgacgcag ccacgtctcc ggggagattc tgaagagcat tgtcgtcttc agatccgcct
    13201 gaatcgcctc gaatcttccg cgccagcttc tccatccgat caagcttctt aatttcgtcg
    13261 gcgtgcgggg aaactacttt tccgacagac gcggctaact gaaacccaag gcgagtgttc
    13321 tctttcgctc gctcaaccat ctccagatgc gcttcctcct ggcgccggat cgcagctcgg
    13381 tggagcatct cagcttcggg gccgaccggc gacggggcct ccttgatcga atttggtatt
    13441 aaaggggaag agttgtcact actcgactgg gcgcgaacgt cagaggtgag gccgaaaata
    13501 aggatacctg tgaagagtag cttttgaaac aacaacttgc tggaaatcaa attagtcata
    13561 acttcagacc gcaggccgaa agacgtaaaa cgtgtcatgc acaaccttct gacgatgaaa
    13621 cgacacgcat tagttgcccc aacctgcgcc cttatctgat gaccgttggc gccgctttgt
    13681 tcgagtcctc aattgatgcg ttgagatttt acctgagctg ttgtcggcat gccgacttct
    13741 aaggcgtaac cttagctttt taacgtatcg gatcggcgtc atcagggctg ttaataaatt
    13801 cttcaccttt tggttttggt ttctgcacga tgattgtttt gccttcgggc gtgcgctcgg
    13861 gtgggcgctt ggcgccgggc agagtattga aagccacttc ctcagcttgg atgaggctcc
    13921 tgaggcgaac tattttcgac gcagcccatt cacgattgga gatggtcaaa gaatagctcc
    13981 ccgtttctcc gggtgagatt gaggcgggcg ttacttgcat ctccttcagc gttcgcccgc
    14041 cgccaacgcg gccggtgagt tctacctcaa caataagatc ggttaatttc tggttagaga
    14101 cattttgaat tgtccccccg atgacggcct gagaaccttt taataaaggc tcgttttcaa
    14161 aaacgttaac agcaggaggc ttggatactc tcgtgttgct ctttaaaccc gaagtcgagc
    14221 caattggccc gggagattca gaattcccac agctaataat agagataatt aaaagaagaa
    14281 cggccgggag aaggaataac tgataaggcg tagaggtgcg ggaaggtcga gggttagtca
    14341 tgacagactt aatgttagag cttaagaaac ttaatccaac cagtggcggg ggagagggta
    14401 aatagataat aaattagagc ttacggctcg tcaatcggct cgtagcctgt cgggggggcg
    14461 ccggtaagat tctggatcag gcggatgaga aattcttgct cgaccgcgcc cgtgctgcgc
    14521 aacgtgaccc actcggcgcc tgtgatgcct tcactccgtc cctcaatctt cgcattgaag
    14581 gagacgtccg tgctggaggc atcaatcgga aaaacttcga ccgtcagagt gtagcgcccg
    14641 cgcgtccatc cacgcgtgtc agacgcgggg acttcggcta cctgactgag gccgccggag
    14701 gcgaaaacca cgccccggat aaaagtaaat ggttgtgtaa tgaggatgcc ttcggtgggc
    14761 tttgaaacat tttcgtcgag aatgagtttg cgctcttgca gcaattccag caaagccttc
    14821 tgaacgacat cgttcggaga ggagaaccga taaggattgg aaagaggtgg tggcgtcggc
    14881 gtgcgaccct tgccgaaatc caccccgcgg cctgccccgg tgtctacctt ccccccgttg
    14941 acggccggcg cccgattcgt actgttatcg cgaatccgct cgttattctg gtcaacgccc
    15001 ttctcctgcc cctgagccac agccgcgaac gacagcatga cgaacaacaa aacacttaac
    15061 ttcctcatat cctgaccctt tcaagaacca ccaagtgcat ataacaacgg ccgcgtatta
    15121 gcttagggga gatgtctatg agaatagaac atcatgtacc tgaatacaag aaagtaatat
    15181 ggggaaggag gctctcatgc gggcgtggca gtaattattg tggtgtagcg gccgtattcg
    15241 tattcgcttg ggcgtcgcgg gttcgctccc actcccgaag tctctgaacg ccgcgctgaa
    15301 agtccgactc ggtggcgtaa aagttatgag cgccgtcgtt tctggatggc tcgcgcacgt
    15361 agtagaggta atcggttgca gcaggagaca atgcggcctc aatcgataac gcgcccggcg
    15421 aagctaacgg cccgggcgga aggccgacga ccttgcgcgt gttatatggt gagtccctgt
    15481 ccagatcgct ctggtagact ttgccgtcgt tgcgccactt gccagccagc tttgaggcgt
    15541 agatgaccgt agagtcgatt ccgagcgcca tgttcagacg cagacggttg tagataactg
    15601 aggcgacaag gggacgttct tcattgagtt tcgcctccgt ctcgaccagc gaagcgacgg
    15661 tgacaatctc gcggggcgac ctgcccgagg agcgcgccac ctccgagaaa cgtccctccc
    15721 agacctgtcg gaacctgctc accatcaggg caatcacatc tcgcgctgaa gtgtccggtg
    15781 gatagctata cgtctcgggg taaagataac cttcgagatt actggcgatg gggtcgatgt
    15841 cggcgatcaa cgagacctcg ttcatcagag ccagtgcttc ctcggggctt tgaagcttca
    15901 actcggggag ccgggccatc tgtgcggcga tgtcccaacg cgtccaccct tctatgacgg
    15961 tcagacgact gagacgctgt tcaccctcct ccagctttct cagtacttca accggcgcaa
    16021 tgggcgaagc gaatctatat tcacccgctt tgagacgtgg tccagacccc gagagtcgaa
    16081 cataaagctt gaagagccat ccacgtctga taacgcctgc gttagtgagc gtgttaataa
    16141 cctcttcggg atttgtgcca cgttttattt ccagatattg gttggcgtgt gtgtgctcgc
    16201 gtgcggtttt aagttctttg taggcccagg cggagaagag ggcagcggac agaattagaa
    16261 gtgtcagaat aattatgaag gctcgcaagg gtcgcatcgt cgtatggtgg ctatctaaag
    16321 ctcttaaaat ttaggatgaa tgtgagatga aatctaacaa gatcaagcga gctgcttcgc
    16381 cgtcgatgcg cccggcaatc gcactctcgg aatatccttc agccctgaga gtttcttctg
    16441 cttccctgga tgtcaaacgc tcgtcctgga aatagaccgg gaggttaagc gaaagttgaa
    16501 ggttacgccc gaagcgacgt gcttcttccg ccgcgtcgcc ctccgcgcct tcgagaagca
    16561 gcggcaaacc aatcaccact gtctttacgt caaaccgcct gacgacatcc gaaagagact
    16621 gaagcaggtc tttccagttc gagcgccgca gtggggagag tgggcgcgcc gtcaaacgca
    16681 gctcgtcgga gacggccaac ccgacgcgtt tgcgcccggg atcgatggcc agcacccggc
    16741 cgctcgtgag tctacccaaa gactccgggc caacttggat gtgttcgtta tcgcgcactt
    16801 gatattgatt tgccgctgaa acgagtatat ctcaaagggg cagacccggc cacccgaatc
    16861 cttgcggctc tcttctcggc tactgtatca tctgcgcgga agcgagttcg tcgcgcagga
    16921 ttttttctgg ctcctccggc aggggatgta attgtagcaa gcgaggtaat ttgatgtctt
    16981 ttcgcggtaa gtttgctctc gtcgtttttt cggcaacact ggcgctttac gcggtcgcgg
    17041 gcggcctgct ttcgggcacc gcgcaacaac agaacgaccc cggcgctcag ctacgcatct
    17101 tcgagagtgt tctgcaccac atccagacgg attatgtgga cgagcctgat ctgggcaagg
    17161 tgagggccgg ggcgctgcgt ggtttggcat acgggcttga cccttattcc acatacctga
    17221 cggtggatca ggttcgcgac tttcgtgccg gggtacagaa cacgcaggtc ggcatcggcg
    17281 cggagctttc gcaggtggct tcttatctct acgtgatcgc gccggtcaaa ggcgggccag
    17341 ccgatcaggc cggtcttctg gcgggtgaca tcgtcgagta catcgacggg aaggcgacgc
    17401 gcgacatctc tctctacgac gcgcggcaac tgttgagcgg agcgccgggc acggaggcga
    17461 agttgcgggt tctgcgtgcg ggcacgcgac ccttcgctct ctcgatcaag cgcgcttcgg
    17521 tggccgtccc cgcggccgag agccggatgg aggcggggca ggtcggcgtg attcgggtca
    17581 acagtctcga cgcgggtgag gcggccgacg tgaaggcgcg tgcgcaggaa ctcatcaaac
    17641 agggcgcgca gaagattctg ctcgacctgc gcagcgtggc cggcggaact ttggccgagg
    17701 gcgtgaaggt cgctaacctc ttcgtccgcg agggcgtcat cgcacagacc gtcaagcgag
    17761 gcaacaagac tgacaaggtc ttcaacgccg acccgaagga ggccatcttc gacggcccgg
    17821 cggccgtcat catcgaccgc gggacggcgg gcgcggccga gatcatcgcc tcggccttcg
    17881 ttgagcgcaa gcgcggcgag gtggtgggcg agaaaagctt cggcgcgggc gccgagcagc
    17941 agttgttcac gctacgcaac ggcgacggcc tgctgctgac gacgatcaag tgggcgtcgt
    18001 cgaacggcaa gccgtttctg cccgagggcg tcaagccgtc tgtggaagtg aagcgaccgg
    18061 aactcgcgga ggccattgac ccggacgagt tgaccggcaa cgacgacgac cccatcgcgc
    18121 agccgaacac gcagggtgac aggcagaacg tcgcgcccga gccggccgcg cccaagcagc
    18181 agcccgagga cgtgcagatg aagaaggcgc tcgaactgct tcgtgagaag gccaagccgg
    18241 cgtcgccgca aaaggcgggc tgagccgctt ttatttcaag gcagagggcc ggggaggatg
    18301 ttcgtccttc ccggcctttg tgtttttaga gcgggatgtt gccgtgtttc ttgggcgggt
    18361 tggtgtcgcg cttgttctcg atgagtgaga gggcgcggat gagcttttgg cgcgtctgcg
    18421 aaggctcgat gatctcgtcg atgaagccgc gctcggccgc cttgtagggg ttggcgaatt
    18481 tctcctcgaa ctcactgatg cgggcgcgtc gcgcagcttc ctgatctttg gcctcgccga
    18541 tctcgcggcg gtagagcacg ccgacggcgc cctcggcccc cataacggcg atctcggcgg
    18601 tcggccaggc gaagttgaca tcggtgcgga tgtgcttgga ggccatgacg cagtaggcgc
    18661 cgccgtaagc tttgcgcgtg atgacggtga ctttggggac ggtggcctcg gcgaaggcgt
    18721 agagaagttt ggcgccgtgg cggatgatgc cgccgtgctc ctgtcggacg ccgggcagga
    18781 agccgggcac gtcctcgaag acgacgagcg ggatgttgaa gcagtcgcag aagcgcacga
    18841 agcgcgcgcc cttgacggag gcgtcgatgt cgagcacgcc ggcgaggaag gcgggctggt
    18901 tggcgacgat gccgacactg cggccgtcga gacgcgcgaa gccgacgacg atgttctgtg
    18961 cgaagtgctc gtgcacctcg aagaagtagc cttcgtccac gacacggtgg atcacgtcac
    19021 ggatgtcgta aggctgattc gacgcctcgg ggacgatggt gtcgaggtcg gcgtcggcgc
    19081 gctcgggcga gtccgagcaa ggcaggcgcg gcgcgtcctc catgttgttc gaggggatga
    19141 aggagagcag ttcgcggacg aggaggaggg cgtgctcgtc ggagtcggcg gagaagtgtg
    19201 cgacgccgga gacgcggttg tgtgtgtcgg ccccgccgag ttcctccttc gacacctcct
    19261 cgtgcgtgac ggtcttaatc acgtcggggc cggtgatgaa catgtaggag gtgttcttga
    19321 ccatcacgtt gaagtcggtg atggcggggc tatagacggc gccgccggcg cacggcccca
    19381 tgatgcaact gatctggggg acgacgcccg aggccagcgt gttgcgaagg aagatgtcgg
    19441 cgtagccgcc cagactgaca acaccctcct gaatgcgcgc gccgcccgag tcgttgagac
    19501 cgatcacggg ggcgccgacg cggatcgcg
//
LOCUS       DASVES010000295        10503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1312663, whole genome shotgun sequence.
ACCESSION   DASVES010000295 DASVES010000000
VERSION     DASVES010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312663"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1377
                     /locus_tag="VF240_11950"
     CDS             97..1377
                     /locus_tag="VF240_11950"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7175964.1"
                     /translation="MANPMVDDTRRVREDNPFESMMSRFDRAAKLLDLNPDLYAVMRV
                     PNRELKVYIPVRMDSGRIEVFEGYRVQHNFARGPAKGGIRYAPDVTIDEVRALAAWMT
                     WKCAVVNVPFGGGKGGVICDPSQMSLGELERLTRRYAAELMDFIGPEKDVPAPDMNTN
                     EQTMAWIMDTFSMHARHTVNAVVTGKPVELGGSQGRREATGRGLLFVINEAIKALKMS
                     PPQTRVVVQGSGNVGGIGARLLHEAGYKVVAISDIFGGIYNPDGIDIHAALKHLQQTR
                     SFDEFPGVERVSNSELLEIDCDVLAPCATENQITSENADKIKCKILAEGANGPTTAAA
                     DEILHRKGVFVIPDILANAGGVTVSYFEWVQNRMGYYWKEQVVNDRLQDIMVASFNDL
                     VRYADAHEVDTRTAAYMLAIDRVAYDTRMRGIYA"
     gene            1828..4269
                     /locus_tag="VF240_11955"
     CDS             1828..4269
                     /locus_tag="VF240_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175965.1"
                     /translation="MRLARRALVAALVTLTLTAAVSAQTLRQENDPRNTAPTVGTGGS
                     PGGPTGLFTIYDGQTLRRGEFTFSVAYSNYDRDPGNVDITEVPVSFQIGVNDHLELFY
                     NTDAYRGVKVNNPQNLSSFYLPNTTHHIGIPFSSPAAIILAPERVSGPTLTTTGSVFR
                     PRGNQPFVQFPFVGGTGPNLGLSGNNIAPPFVSRLGVPTGSEQGNFSSAANFPGVGSV
                     YGSILPGIVLTTRTIPANLTFNPLTVPDLFTAAPSYLPDAPFISRLYGESAFSTHTFG
                     ANFRLTEADNALSVNLIPMYRWYWDKADDFSGFNQLQRGASPGGDIGDFGLFAVVGGR
                     LSRSVHLAANFGFFLNSNPQSEAFGTGKVTLLDRANEFVAGVGADFPVTKHFQLMAEL
                     KSIRYVGGRTPNAFPNNPVETLMGVRIYPARWWGMSAWYRAHLNQQGDRPYSNTDYPA
                     GFQESSDPHGFGFQIFAGRRNERAPAILPNQPPVVTLTSSSSRVVLAAECGPNQRPST
                     TCTPTDTSVRLSAQATDPDGDTLLYTYSTSGGRVVGDGPNATLDMSGVAAGTYTVTVE
                     VDDGCGCISFAETTVTVERCPCEEVPPVIACPTIDVSCPDTVKGGDPITFTASVSGGD
                     AAVTPTFNWTVSAGTISSGQGTSSITVDTTGLPANSTVTATVDVGGYDRTCTGSDSCT
                     TGIQIAAEARKIDEYGNIRFNDEKARLDNYAIALQNEPGAQGTIICYGGRVGRAGEAQ
                     ARCDRAKNYLVNERGIDASRVSTVDGGFREELTVELWVVPTGAQPPTASSTVDPSEVR
                     TTRPSRRGRRRGREE"
     gene            4554..6950
                     /locus_tag="VF240_11960"
     CDS             4554..6950
                     /locus_tag="VF240_11960"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX7175966.1"
                     /translation="MMNKLTRLFLLLALAAMLATPFAASAADDDDSNSDAERVMGWDV
                     TGPMGGDVRSLAIDPQDPQRLYFGTIDGQIYTSADGGATWERLGGFNRPGLYIDNLIV
                     DPDNSKIIFAAAHRHKEPGGFYKTTDGGVTWKESAELKGEGIHSLTQSPKEPDILVAG
                     TNRGVFRSDDAGDSWKHLNTAATPGLVNVESLAIDPRDSNVIYAGTWYLPYKTTDGGQ
                     NWSITKTGIIDDSDIFAIEIDQTNPDHVIMSACSGIYETKNAGALWRKVNGIPSQSRR
                     TRAILQNPAVPSAIYAGTTEGFWLSQNGGDDWRVTTNRQNFEVNAIAVHPKNPQTIYI
                     GTNNFGVMVSRDGGKTFTPSNEGYSGRRAYFILPDRETPNRVYATTINTATGGGFFYV
                     SNDGGETWQPSTRNMPNRLISYAILQDQNEANVIYLGTNYGIYRSADRGGTWAPIMPP
                     KPKPAPRSKRGRGKAAPATASSQAAAPKPATPDQKDQIKRAQSALNALGYDVGTPDGV
                     AGTRTISALRRFQAEKAIPQTGTFDDVSLAALGLAGGMQTAGGASQAMQSAPLFFNEK
                     DKEVTVNALAHTFDERDGRPGMFAATHNGLYRTYDPAAGWQRISYGDAFDAEPRTLCI
                     AMSAVQPETIYVGTAKLGVLVTRDGGRTWEQVRAIPTDAPINVIEQDPKRATHVYVGT
                     TQTFYISHDGGERWQRRGGNLPLGSFTSVLINPENTDELFVGNAYERGGRSFTTGEGG
                     GVFRSTDAGMTWQRIDPQLPSRRVWALALDPRDSGRLFVGSHSAGIYVARRPANSAAR
                     "
     gene            7379..9475
                     /gene="rpsA"
                     /locus_tag="VF240_11965"
     CDS             7379..9475
                     /gene="rpsA"
                     /locus_tag="VF240_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7175967.1"
                     /translation="MSTVDNNSPDGETDNTSNQSAAARSPEPAAEATAGDTPAQNVTA
                     TSGASAATATEPETQPTAATPAEAPPPVAETPAEQPSAEPSPAAATPAAPPIEAPAAT
                     TAAAADDDDDDEGEGGNRSTGFDFGAILDKYEQEQAAYQEGSVVRGNVVDINERGVVI
                     DFGFKSEGVVNPEEFMENGELTVKRGDEVEVLIKSMESQEGVPILSRADAVRMRAWDD
                     LEKAYQDGTPVKGRIVERVKGGLRVDVDGVAAFLPGSQVDVRPIRNLDSLRNQEIEAL
                     VIKLNRKRSNVVLSRKAIIEAQNVGKKTQTLDHLEEGIIVEGQIKNLTDYGAFVDLGG
                     IDGLLHVTDMSWGRLQNPAEMFRVGENVQVKVLKFDRERERVSLGFKQLLPDPWESVQ
                     ERFEINARMQGKIASVTDYGAFIELEPGVEGLVHVSEMSWSKRMKHPSKLVNVGDEVE
                     VEVLGVDPRARRISLGMKQAQPNPWESLKARFHVGDRVRGRVRNLTDFGAFVEIEDGV
                     DGLVHVSDISWNKRIKHPGEALKKGQEIEAVITNIDTENRRLSLSIKDMEPSAWEKFT
                     TEHRPGDIVHGRITRFANFGAFVELAEGLEGLCHVSELSDDRVEKPEDVAKIGDEMDF
                     RILRIEGESKKIGLSHRAVSDESGGAVQAETKQYSTDAGGGMASLGELADFFNKRGGS
                     SGDSGGGDGEDESDSR"
     gene            9745..10011
                     /locus_tag="VF240_11970"
                     /pseudo
     CDS             9745..10011
                     /locus_tag="VF240_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            10193..>10503
                     /locus_tag="VF240_11975"
     CDS             10193..>10503
                     /locus_tag="VF240_11975"
                     /inference="COORDINATES: protein motif:HMM:NF016555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7175968.1"
                     /translation="MDRLYTPWRYDYIKGATGEKSGPDAACVFCALRDRPDDDERNYV
                     VTRARHNFIVLNIYPYVSGHLLIVPLEHAPDLDAVSKQATDELMDLSKRALVLLRDAY
                     RP"
BASE COUNT         1984 a         3594 c         3233 g         1692 t
ORIGIN      
        1 gataagtgat gagtgataag tgataagaaa ggcaaacccg ctttcttccc tcttatcact
       61 tatcactcat cacttatcac ctgcttggag aagaaaatgg caaatccgat ggtggacgac
      121 acgcggcggg tgagagagga caacccgttc gagtcgatga tgtcgcgctt cgaccgcgcg
      181 gccaagctgc tcgacttaaa ccccgacctc tacgccgtga tgcgcgtccc gaaccgcgag
      241 ctgaaggtct acatccccgt gcggatggac tcggggcgca tcgaggtctt cgagggctac
      301 cgcgtccagc acaacttcgc gcgggggccg gccaagggcg gcatccgtta cgctcccgac
      361 gtgaccatcg atgaggtgcg ggcgctcgcc gcctggatga cgtggaagtg cgccgtcgtc
      421 aacgtcccct ttggcggcgg caagggcggc gtcatctgcg acccgtcgca gatgtctctg
      481 ggcgagctcg agcgcctgac gcgtcggtac gccgccgagc tgatggactt catcggcccc
      541 gagaaggacg tgcccgcgcc cgacatgaac accaacgagc agacgatggc gtggatcatg
      601 gacaccttct cgatgcacgc gcgccacacc gtcaacgccg tcgtcacggg caagcccgtc
      661 gagctgggcg gctcccaggg gcggcgcgag gcgacggggc gtggcctgct cttcgtcatc
      721 aacgaggcca tcaaggccct gaagatgtcg ccgccgcaga cgcgcgtcgt cgtgcagggg
      781 tcgggcaacg tcggcggcat cggcgcgcgt ctcctccacg aggccggcta taaggtggtc
      841 gccatctccg acatcttcgg cggcatctac aaccccgacg gcatcgacat tcacgccgcc
      901 ctcaaacatc tccaacagac acgctccttc gatgagtttc ccggcgtcga gcgcgtctcg
      961 aactccgaac tgctggagat cgactgcgac gtgctcgccc cgtgcgccac ggagaaccag
     1021 atcacgtcgg aaaacgcgga caaaatcaaa tgtaaaatcc tcgccgaggg ggccaacggg
     1081 ccgacgacgg ccgccgccga cgaaatcctc caccgcaagg gtgtcttcgt catcccggac
     1141 attttggcca acgccggagg cgtcacggtt tcctacttcg agtgggtgca aaaccgcatg
     1201 ggttactact ggaaagagca ggtcgtcaac gaccggcttc aggacatcat ggtcgccagc
     1261 ttcaacgacc tcgtccgcta tgccgacgcc cacgaagtgg acacacgcac cgccgcctat
     1321 atgctggcaa tcgaccgcgt ggcctacgac acgaggatgc gcggcatcta tgcgtgaaac
     1381 aaaaatgtgt caaacttttc cggctaagtt gcacaaactt cttgatttcg gagcaaatgt
     1441 ggagtatggt ctggcggaaa ttggtgtgcc gaagccgcgt tgtgtgaagt cgaacagacg
     1501 aatgcggcgg ccggtcacac gggggctgtc gtcattgaca gcggcgcgtt ctgttcggta
     1561 taagcagtaa gcgccaaagc gcaagttcct aaagagaact acagtttaga ggctccggga
     1621 agatgcccgg acggccggcc gaaggtggtt cggcccctct cgctgctccg ctccagctac
     1681 aaagaattaa cggtttgagg gcgtgagcag ttcttctttg actttcaggg tttgtagttg
     1741 gagcgtccgc gcccttcgtg cttcccgcac gggagagact ggcgccccct tcagcaaatt
     1801 ataaactcat ttcgcaggag gaatgcgatg agactcgcga gaagagcgct cgttgccgcg
     1861 ctcgtcactt tgacgctgac ggcggctgtg tcagctcaaa cacttaggca ggagaatgat
     1921 ccccggaaca cggctcctac cgtcggcacg ggcggctcgc cgggcggtcc gaccggactc
     1981 ttcaccatct acgacggcca gacgctgcgc agaggtgagt tcaccttcag cgtcgcctac
     2041 agtaattacg accgcgatcc cggcaatgtg gacattaccg aggttccggt cagcttccag
     2101 attggcgtca acgaccatct ggagctgttc tacaacacgg acgcgtaccg cggggtgaag
     2161 gttaataacc cgcagaacct gtccagcttc tacctgccga acacgacgca ccacatcggc
     2221 atcccgttct cgagcccggc cgccatcatt ctcgcgcccg agcgcgtgag cggccccacg
     2281 cttaccacta cggggtcggt cttccgcccg cgtggcaacc agccgtttgt gcagttcccg
     2341 ttcgtcggcg ggacggggcc gaacctcggc ctctcgggca acaacatcgc cccgccgttt
     2401 gtgagccgcc tcggcgtccc gaccggcagt gagcagggta acttcagctc ggcggcgaac
     2461 ttccccggcg tcggctcggt gtacggctcc atcctgccgg gcatcgtgtt gacgacgcgg
     2521 acgatcccgg ccaacctgac gttcaacccg ctgacggtgc ccgacctgtt caccgcggcg
     2581 ccgtcctacc tgcccgacgc gcccttcatc agccgcctct acggcgagtc tgccttctcg
     2641 acgcatacct tcggcgcgaa cttccggctg acggaggccg acaacgcgct ctcggtcaac
     2701 ctcatcccga tgtaccgctg gtactgggat aaggccgacg acttctcggg cttcaatcag
     2761 ctccagcgcg gggccagccc cggcggcgac atcggcgact tcggcctctt cgccgtcgtc
     2821 ggcggacgcc tcagccgctc ggtgcacttg gcggccaact tcggcttctt cctgaacagc
     2881 aacccgcagt ccgaggcctt cggcacgggc aaggttaccc tgctcgaccg ggccaatgag
     2941 ttcgtcgcgg gcgtcggcgc cgacttcccc gtcaccaagc acttccagtt gatggccgag
     3001 ttgaagtcga tccgctacgt gggcggcagg acgccgaacg cgttcccgaa caacccggtg
     3061 gaaaccctca tgggtgtgcg tatttacccg gcgcgctggt ggggtatgag cgcctggtat
     3121 cgcgcccatc tcaaccaaca gggcgaccgg ccgtacagca acacggacta cccggcgggc
     3181 ttccaggagt cgagcgaccc gcatggcttc ggcttccaga tcttcgccgg acgccgcaac
     3241 gagcgcgccc cggccatcct gccgaaccag ccgcccgtcg tcacgctcac ctcctcgtct
     3301 tcgagggtgg tgctcgcggc cgagtgcggc ccgaatcaga ggccgtcaac aacctgcacg
     3361 ccgactgaca cgagcgttcg tctctcggcg caggccactg acccggacgg cgacacgctg
     3421 ctctacacct actcgaccag cggcggtcgc gtcgtcggcg acggcccgaa cgcgacgctc
     3481 gacatgtcgg gcgtggcggc gggcacctat accgtcacgg tcgaagtcga tgacggttgc
     3541 gggtgcatct cctttgccga gaccacggtc acggtcgagc gctgcccgtg cgaggaagtc
     3601 ccgccggtga ttgcctgccc gaccatcgac gtgagctgcc ctgacaccgt caagggtggc
     3661 gacccgatca ctttcacggc ctccgtcagt ggcggcgacg cggcggtgac tccgaccttc
     3721 aactggactg tctcggccgg cacgatctcc agcggccagg gcacgtcctc aatcacggtt
     3781 gacacgaccg gcctgccggc caactcgact gtgaccgcga ccgttgatgt cggcggctac
     3841 gaccgaacct gcaccggctc ggattcctgc acgacaggca tccagatcgc ggccgaggcg
     3901 aggaagatcg acgagtacgg caacatccgg ttcaacgacg agaaggcgcg ccttgacaac
     3961 tacgccatcg cgctccagaa cgaaccggga gcgcagggca ccatcatctg ctacggcggc
     4021 cgtgtcggcc gtgcgggtga ggctcaggcg cgttgcgacc gtgcgaagaa ctacctcgtc
     4081 aacgagcgtg gcatcgacgc ctcgcgcgtc tccaccgttg acggcggctt ccgcgaggag
     4141 ctgacggtcg aactctgggt tgtcccgacg ggcgcccagc cgccgaccgc tagctcgacc
     4201 gtggacccga gcgaggtgcg gaccacccgt ccctcgcgcc ggggccgtcg ccgtgggcgt
     4261 gaagagtaac gcctgacgct cgcgccgccc tcttccgggc gtgcgtgacc ccttccgaaa
     4321 gcgggcgatg cttcacacga agcatcgccc gcttcgcttt tatgggcagg ccatgcgccc
     4381 cggcagcggc gtacaactgc cccgtgcggg gtggcatcct aaactctcgg ccgccggctc
     4441 tcctccggtt tttgcaaaaa accttcacgg cagggataat cagtggttcg cgcgcgggcc
     4501 gtgcgccgcg ctctaacact gtagaggtct tgaaagcccc gacaatgatc cgaatgatga
     4561 acaaactcac cagactcttt ctgctgctcg cgctcgcggc gatgctcgcc acgcccttcg
     4621 cggcctccgc ggccgacgac gacgactcga actctgacgc cgagcgcgtc atgggctggg
     4681 acgtgaccgg gccgatgggc ggcgatgtgc gctcgctcgc catcgacccg caggacccgc
     4741 agcgtctcta cttcggcact atcgacggcc agatttacac ctcggcggac ggcggcgcga
     4801 cctgggagcg gctcggcggc ttcaaccgcc ccggcctcta catcgacaac ctcatcgtcg
     4861 atcccgacaa ctccaagatc atcttcgccg cggcacaccg ccacaaagaa cccggcggct
     4921 tctacaagac gactgacggc ggcgtcacct ggaaggagtc ggccgaactc aagggcgagg
     4981 gcatccactc gctcacgcag tcgcctaaag aaccggacat cctcgtcgcc ggcaccaacc
     5041 gcggcgtctt ccgctccgac gacgcgggcg actcctggaa gcacctcaac actgccgcga
     5101 ctcccggtct cgtcaacgtc gagtcgctcg ccatcgaccc gcgcgactct aacgtcatct
     5161 acgccggcac gtggtatctg ccctacaaga ccaccgacgg cggccagaac tggagcatca
     5221 cgaagaccgg catcatcgac gactcggaca tcttcgccat cgagatcgac cagaccaacc
     5281 ccgaccacgt catcatgtcg gcgtgcagcg gcatctacga gacgaagaac gccggcgctc
     5341 tctggcgtaa ggtcaacggc atcccttcgc agtcgcggcg cacgcgcgcc atcctccaga
     5401 acccggcggt gccgtcggcc atctacgccg gcacgaccga gggcttctgg ctgtcgcaga
     5461 acggcggcga cgattggcgc gtgaccacca atcgccagaa tttcgaagtc aacgccatcg
     5521 ccgtccaccc gaaaaacccg cagaccatct acatcggcac gaacaacttc ggcgtgatgg
     5581 tctcgcgtga tggtggcaag acgttcaccc cgtcgaacga aggctactcg gggcggcgcg
     5641 cctatttcat cctccccgac cgcgagacgc cgaaccgcgt ctatgcgacg accatcaaca
     5701 cggccaccgg cggcggcttc ttctacgtca gcaacgatgg cggcgagacc tggcagccct
     5761 cgacgcgcaa catgccgaac cgcctcatct cctacgctat ccttcaagac cagaatgaag
     5821 cgaacgtcat ctacctcggc acgaactacg gcatctatcg ctcggccgac cgggggggca
     5881 cgtgggcgcc aatcatgccg ccgaagccga agcccgcgcc gcgctcgaag cgtgggcgag
     5941 ggaaggccgc gcccgcgacg gccagctcac aggccgccgc gccgaagcct gccacgcctg
     6001 accagaaaga tcagattaag cgtgcccaga gtgcgctcaa cgcgctgggc tacgacgtgg
     6061 gcacgccgga tggcgttgct ggcacgcgta ccatctcggc cctgcgccgc ttccaggccg
     6121 agaaggccat cccgcagacc ggcacgttcg acgacgtgtc gctcgccgcc ctcggcctcg
     6181 ccggcggcat gcagacagct ggcggcgcgt ctcaggccat gcagtcggcg ccgcttttct
     6241 tcaacgagaa ggacaaggag gtcaccgtca acgcgctggc ccacaccttc gacgagcgcg
     6301 acgggcgacc cggcatgttc gccgccaccc acaacggact ctaccgcacc tacgacccgg
     6361 ccgccgggtg gcagaggatt tcctacggcg acgccttcga cgccgaaccg cgcacgctct
     6421 gcatcgcgat gagcgcggtg caacccgaga ccatctacgt ggggacggcc aagctgggcg
     6481 tcctcgtcac gcgcgacgga ggccggacgt gggagcaggt gcgcgccatc cccacggacg
     6541 cgcccatcaa cgtcatcgag caggacccga agcgtgcgac gcacgtctac gtcggcacca
     6601 cgcagacctt ctacatctcg cacgacggcg gagagcgttg gcagcgtcgc ggcggcaacc
     6661 tcccgctcgg cagtttcacg agcgtgctca ttaaccccga gaacacggac gaactcttcg
     6721 tcggcaacgc ctacgagcgc ggcgggcgct ccttcacgac cggcgagggc gggggcgtct
     6781 tccgttccac tgacgccggt atgacgtggc agcgcatcga cccgcaactc cccagccggc
     6841 gcgtctgggc gctcgccctc gacccgcgcg actcaggccg gctcttcgtc ggctcccact
     6901 cagcgggcat ctacgtcgcg cgccgcccgg ccaactccgc cgccagataa ggcaaaagtc
     6961 aaaagtaaaa aggcaaaagt gagagagcgg tttcgccctt cacttttgcc ttttgccttt
     7021 ttacttttgc cttacttctg cccgcaacgc cttgacacgt cgcggttaac gcgtgtaaat
     7081 tgccagccta cccggtcatc gaagaccgtt tgattggcgg gtcacgcgga tgatccgaag
     7141 gcacaaggta accgaaactt ttggcaagct gtttcaaggg ctcagacgat cacccgaacc
     7201 cccgccttaa atccttcgcc tcccgtcaat catcgagttt tttaagccgt agcgtcaaag
     7261 tccccgaacg cctccgggcc actcacccaa aggctttgac gacgcgcgtg accgggtgcg
     7321 gcgccggccc ttacatcggc cgcgccgact tcacggcagg agtccgacag gagttcgcat
     7381 gtcaaccgta gataacaact cgcccgacgg cgagaccgac aacacctcga accagagcgc
     7441 cgccgctcgt tcgcctgagc cggccgctga agcaacagcg ggcgacacgc cagcgcagaa
     7501 cgtcaccgcg acgagtggcg cctccgccgc tacagcgacc gagccggaaa cgcagccaac
     7561 cgctgccacg ccggccgaag cgccgccgcc cgttgccgag accccggccg agcagccgtc
     7621 ggccgagccg tcgccagccg ccgccacgcc ggccgctccg cccatcgaag cgcccgccgc
     7681 gacgaccgcc gccgccgccg atgacgacga tgatgacgaa ggcgagggcg ggaaccgctc
     7741 gaccggcttc gacttcggcg ccatcctcga caagtacgag caggagcagg ccgcctatca
     7801 ggaaggctcg gtcgtgcgcg gcaacgtcgt ggacatcaac gagcgcggcg tcgtcatcga
     7861 cttcggcttc aagtcggagg gcgtcgtcaa ccccgaggag ttcatggaga acggggagtt
     7921 gaccgtcaag cgcggcgacg aggtcgaggt gctcatcaag agcatggaat cgcaggaggg
     7981 cgtgcccatc ctctcgcgcg ccgacgccgt gcggatgcgc gcgtgggacg atttagagaa
     8041 ggcttatcag gacggcacgc ccgtcaaggg ccgcatcgtc gagcgcgtca agggcggatt
     8101 gcgcgttgac gttgacggcg tcgccgcctt cctccccggc tcgcaggtgg acgttcgacc
     8161 catccgcaac ctcgactcac tgcgtaatca ggagatcgag gccctcgtca tcaagctcaa
     8221 ccgcaagcgt tcgaacgtcg tgctctcgcg caaggccatc attgaggcgc agaacgtcgg
     8281 gaagaagacg cagacgctcg accatctgga agagggcatc atcgtcgaag gccagatcaa
     8341 gaacctgacc gactacggcg ccttcgtcga tctcggcggc atcgacggcc tcctgcacgt
     8401 cacggacatg tcgtggggtc gccttcagaa cccggccgag atgttccgcg tcggcgagaa
     8461 cgttcaggtc aaggtcttga agttcgaccg cgagcgcgag cgcgtctctt tgggcttcaa
     8521 gcagctcctg cccgacccgt gggagagcgt gcaggagcgc ttcgagatca acgcgcgcat
     8581 gcaaggtaag atcgcctcgg tcacggacta cggcgccttc atcgagttgg agccgggcgt
     8641 cgaaggtctt gtgcatgtct ccgagatgag ctggtcgaag cggatgaaac atccgtcgaa
     8701 gctcgtcaac gtcggcgacg aggtcgaggt cgaagtcctc ggcgttgacc cgcgcgcgcg
     8761 ccgcatcagc ctcggtatga agcaggcgca gccgaacccg tgggagtctt tgaaggctcg
     8821 cttccacgtc ggcgaccgcg tccgcggccg cgtccgcaat ctgacggact tcggagcgtt
     8881 cgtcgagatc gaggacggtg tggacggcct cgtccacgtc tccgacatct cctggaacaa
     8941 gcgcatcaag caccccggcg aggctttgaa gaaggggcag gagatcgaag ccgtcatcac
     9001 gaacatcgac acggagaacc gccggctgtc gctttcgatc aaggacatgg agccgagcgc
     9061 gtgggagaag ttcaccacgg aacaccgccc cggcgacatc gtccacgggc gcatcactcg
     9121 cttcgccaac ttcggggcgt ttgtcgagct ggccgagggc ttggaaggtc tctgtcatgt
     9181 ctccgagctt tccgacgacc gcgtggagaa acccgaggac gtggccaaga tcggcgacga
     9241 gatggacttc cgcatcctcc gcatcgaggg cgagtcgaag aagatcggtc tgtcacaccg
     9301 cgccgtcagc gacgagagcg gcggggcggt tcaagccgag accaagcagt attcgaccga
     9361 tgccggcggc ggcatggcct ccctcggcga gctggccgac ttcttcaaca agcgcggcgg
     9421 cagtagtggc gacagcggcg gcggtgacgg tgaggacgag tccgacagcc gctaaagccg
     9481 tttgaaaacg ccgcacgggc acaccaggcc ccgcgcacgc tttcgcgaga gtgaaggtgt
     9541 gcgcggggct tttccttttc ggccccccgc cgtgtactgc taaatcgttg gcgcacaagg
     9601 gacttttccc ttgctacaag tcgccgtctt ttgataactt actgcccgcg ctccgaaact
     9661 tctgtgccca cgggccgacc ccgcggggcc gcgtgcggcg ccggttgttt cctttagcga
     9721 gacttgagag ggccaatgaa aagcatgacg aaagccgagt tggtcgaaga cgtggcgcgc
     9781 gcggccgagt tgaccaagaa ggacgccgag cggctggtcg agatcgtctt cgagagcatc
     9841 atcgaaacgc tcaacaaggg cgagaagatt gagctgcgcg gcttcggcag cttccgcgtg
     9901 cgcgagcgcg gcgcgcgccg cgggcgcaac ccgaagacgg gcgaccccgt ggacatcccc
     9961 gccaagcgcg tcccctactt caagcccggc aaggagttga aggagctgat caacgaggag
    10021 ccggccgcgc cacctacggg ggcgtccgag gcttccacgc ccgccgcgac ggagcgcgag
    10081 gagccgcggg acgagccggg cggcttcggc ggaagcggcg gcttcggcgg cggcggcgga
    10141 cagaccccgc cgctcatgtg aagctccggg aggcgcttgg ggtaaggagt cagtggacag
    10201 gctatacacg ccctggcgtt acgactatat caagggggcg accggcgaaa agtccggccc
    10261 cgacgccgcc tgcgtcttct gcgccctgcg cgaccgcccc gacgacgacg agcggaacta
    10321 cgtcgtcacc cgcgcccgac acaacttcat cgtcctcaac atctatcctt acgtcagcgg
    10381 ccacctcctc atcgtcccac tcgaacacgc ccccgacctc gacgccgtct cgaagcaggc
    10441 cacggatgag ctaatggacc tctccaagcg cgctctcgtc ctgctgcgcg acgcctatcg
    10501 ccc
//
LOCUS       DASVES010000296         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1217590, whole genome shotgun sequence.
ACCESSION   DASVES010000296 DASVES010000000
VERSION     DASVES010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217590"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /gene="rfaE1"
                     /locus_tag="VF240_11980"
     CDS             complement(<1..944)
                     /gene="rfaE1"
                     /locus_tag="VF240_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX7175969.1"
                     /translation="MTTQRAAEIVERAGRSTVVVVGDVMLDEFVWGDVTRISPEAPVP
                     VVDVRRESVRLGGAANVLANLVALGARACVVGVVGRDAAGERLRAELRERGALRAEET
                     LITDDARPTTTKTRIIAHNQLVVRADRERRAPVDPVVEARLVEVVAGLLAGADALVVS
                     DYDKGAVTPRVLGHVLALARSLGKPALVDPKLRNFDSYRPATLITPNHHEALRMSNTE
                     DDTDAGMERAARLIRERLGCESVLITRGEQGLMLLDGAGEPVYVPTVAREVYDVTGAG
                     DTVIATLAAALSAGATLVEAAMLANHAAGIVVGKLGTAT"
     gene            complement(980..2143)
                     /locus_tag="VF240_11985"
     CDS             complement(980..2143)
                     /locus_tag="VF240_11985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7175970.1"
                     /translation="MKSLRSQLEVGAHHAAPPPAPVPAPAAESPRSLAPARWDWGAVR
                     RVLVVRLRSIGDTVLATPSLYALRRFLPAARIDLLLEDWVAPLVEGSTEIDRIVPVER
                     KSNASRLSVARQLRAENYDVAYNLHGGSTATLLTRASGARHRVGYASYRYARLHNHAA
                     PHPSELWRQEQTHSAEQQLALLGWTGVPVTDRPATRLPVTAEATERIARRLHDAGLDA
                     PAQRLALIHPAAAFATKTWAAANFARICEQLAARGISAVAVVAPNEKAVADALVSESR
                     SPVVVFDGLSLPELTALAARASLFVGNDSGVAHIAAAVRVPSVVVFGSSNVAHWRPWS
                     EAPSEVVREELPCQPCPGYTCAEFDAPECIRRVPVERVAAAVERVLAAAGAAA"
     gene            complement(2118..2318)
                     /locus_tag="VF240_11990"
     CDS             complement(2118..2318)
                     /locus_tag="VF240_11990"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX7175971.1"
                     /translation="MSSDLQEFIETFNVICPVCRGKVVLKEGGVGILCTECRRVYPVE
                     NGIPALVAEEAVVENEEPPKSA"
BASE COUNT          411 a          905 c          934 g          370 t
ORIGIN      
        1 gtggccgtgc cgagcttgcc gacgacgatg ccggcggcgt ggttggcgag catcgccgcc
       61 tcgaccagtg tggccccggc cgacaaggcg gcggcgagcg tggcgatgac ggtgtcgccc
      121 gcgcccgtca cgtcgtagac ctcgcgcgcc accgtcggga cgtagaccgg ctcgcccgcg
      181 ccgtcgagca gcatcaaccc ctgctcgccg cgcgtgatga gcaccgactc gcaacccagc
      241 cgctcacgga tgagccgggc ggcgcgctcc atcccggcgt ccgtgtcgtc ctccgtgttc
      301 gacatgcgca gcgcctcgtg gtggttcggc gtaatcagcg tggccgggcg atacgagtcg
      361 aagttacgca gtttcggatc gacgagcgcg ggcttgccaa ggctccgcgc gagcgcgagc
      421 acgtggccga ggacgcgcgg cgtcacggcg cccttgtcgt agtcggagac gacgagcgcg
      481 tccgcccccg cgagcaaccc ggcgaccact tcgacgagac gcgcctcaac gactgggtcc
      541 accggcgcgc ggcgctcgcg gtcggcgcgc accacgagtt gattgtgcgc gatgatgcgt
      601 gtcttcgtcg tggtcggacg cgcgtcgtcc gtgatgagtg tttcttcggc gcggagtgcg
      661 ccgcgctcgc gcagctccgc gcgcagccgc tcgcccgccg cgtcgcgccc gacgacgccg
      721 acgacgcagg cgcgcgcgcc cagcgcgacg aggttcgcca gcacgttggc cgccccgccg
      781 aggcgcaccg actcgcgccg cacgtccacc acgggcaccg gcgcctccgg cgagatgcgc
      841 gtcacgtcgc cccacacgaa ctcgtcgagc atcacatcgc cgacgacgac gaccgtgcta
      901 cggccggcgc gttcgacgat ctccgccgcg cgttgtgtgg tcaattgatt catcgaagaa
      961 tgagtagcct tccgcccctt cacgcggccg cgccggcggc ggccagcacg cgctcgacgg
     1021 ctgcggcgac gcgctcgacg gggacgcggc gaatgcactc gggcgcgtcg aactcggcgc
     1081 aggtgtagcc ggggcacggc tggcagggca actcctcgcg gacgacctcc gagggcgctt
     1141 ccgaccacgg ccgccagtgc gccacgttcg atgagccgaa gacgacgacc gacggcacac
     1201 ggacggccgc cgcgatgtgc gccaccccgc tgtcgtttcc gacgaagagc gacgcgcgcg
     1261 cggcgagcgc cgtcagctcc ggcagcgaga ggccgtcgaa gaccacgacc ggcgagcgcg
     1321 actcgctgac gagcgcgtcg gcgactgcct tctcgttggg cgcgacgacg gccaccgcgc
     1381 tgatgccgcg cgcggcgagc tgctcgcaaa tgcgcgcgaa gttcgcggcg gcccacgtct
     1441 tcgtcgcgaa ggcggcggcc gggtggatga gtgcgaggcg ctgcgcgggc gcgtcgagtc
     1501 ccgcgtcgtg caggcgtcgg gcgatgcgct ccgtggcttc ggctgtgacg ggaagacgcg
     1561 tcgccggccg gtcggtgacg ggcacgcccg tccagccgag gagcgcgagc tgttgctcgg
     1621 ccgagtgtgt ctgctcctgc cgccacaact ccgacgggtg cggcgcggcg tggttgtgca
     1681 ggcgcgcgta gcggtagctg gcgtagccga cgcgatggcg cgcgcccgag gcgcgagtca
     1741 gaagcgtggc cgtcgagccg ccgtggaggt tgtaggccac gtcgtagttt tcggcgcgaa
     1801 gctgtcgcgc gaccgagagc cgcgacgcgt tgctcttgcg ctctacgggg acgatgcgat
     1861 caatctccgt cgagccttcg acgagcggcg cgacccagtc ttcgagcagg aggtcgatac
     1921 gcgcggcggg gaggaagcgg cggagcgcgt agagcgaagg cgtggccagc accgtgtcgc
     1981 cgatggagcg caggcgcacg acgaggacgc gccgcaccgc gccccagtcc caacgcgcgg
     2041 gcgcgagtga gcgcggcgac tccgcggcgg gtgccggcac gggcgcgggc ggcggggcgg
     2101 cgtggtgcgc ccccacctca agctgacttc ggaggctctt cattctcgac gacggcctcc
     2161 tcggcgacga gcgccgggat gccgttctcg accggataga cgcggcggca ctcggtgcaa
     2221 agtatcccta ctccaccctc cttgagcacc accttgcccc gacagacggg acagatgacg
     2281 ttaaaggtct cgatgaactc ttgcaggtcg ctcgacatca cgaaccctcc gcccactgtc
     2341 tccgcgttga ttaagctctg gcaagacgcc caaagattaa cagcatcgtg aagacacggg
     2401 caagaaagcg gtcggtatgg ataactcaac gtcgggtgaa acgttacaac gagccgcgca
     2461 agcggcggaa gaatgtagcc cacggcgcga gccgtgggaa taggacgcaa aataattcga
     2521 agccccagag gggcgaaaga atctcgacgt tgagcgtgat gcccgtcgaa attctttcgc
     2581 ccctccgggg cttaccaaat tttctcaccg ctgttccacg
//
LOCUS       DASVES010000297         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1566303, whole genome shotgun sequence.
ACCESSION   DASVES010000297 DASVES010000000
VERSION     DASVES010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566303"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VF240_11995"
     CDS             <1..838
                     /locus_tag="VF240_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7175972.1"
                     /translation="RTLVGRLEPVIGEALAVGLLTTDQSTDGGINAQRELVAALKAKV
                     KDNFEPEARDLLSVADALVRKTVWIVGGDGWAYDIGFGGLDHVIASGARVRILVLDTE
                     VYSNTGGQASKATPLGAVAKFAAGGKTTAKKDLGMMATKYGNVYVAQVAMGASDTQTV
                     KAMLEAEAHDGPSLIIAYSQCIAHGIDIAKGMHHQKLAVESGYWPLYRYNPALADEGR
                     NPFQLDSGDPKIPLQDYIYTEARYRMLQQSDPAVAKFLLGRAQAAVNAKWEQYKQMAE
                     RT"
     gene            923..1924
                     /locus_tag="VF240_12000"
     CDS             923..1924
                     /locus_tag="VF240_12000"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX7175973.1"
                     /translation="MDLSTTYLGLPLKNPVVASASPLSSTLEGIRRLEDAGTAAVVMH
                     SLFEEQIYLESVSLSDQLDRGSYSTAEALNYFPEMQSYGVGPEKYLELIHAAKLTVGV
                     PIIGSLNGTTPGGWIEYARLIEDAGADALELNVYFIPANPYVTGEAVERRYLDILRDV
                     KRTVSIPVAMKLSPYFSSIGDMSRRLVDEGADALVFFNRFYQPDFDLEKLEVEPRLIL
                     SRSHELRLPLCWVGILYGELLTDFAITSGVHTYEDVLKSLMAGAKVAMMASELLQNGV
                     GRVPVILGQMSEWMERNEYESVRQMIGSMSRTNVADPAAFERANYMKMLASYQRNRG"
     gene            1997..2374
                     /locus_tag="VF240_12005"
     CDS             1997..2374
                     /locus_tag="VF240_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175974.1"
                     /translation="MSKDERDVLELLKAELDYIESGGYGRSVRTPWRPTSVFQDSLTC
                     INFGYPYRAHPCGECFLDALVPPESRTAEVPCHHIPLDAEGTTVEAVESEGNQAMLEE
                     KVKAWLRAKIREIEGERALAVER"
     gene            2385..>2700
                     /locus_tag="VF240_12010"
     CDS             2385..>2700
                     /locus_tag="VF240_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175975.1"
                     /translation="MRLAHPSLSSFTRRLAVVAFGLWLGGAGCAFCCASAENPARVQD
                     ATSSAGAQADSTIFAPTHCPAHARASAGTTDGFERDATSVQTPSPTDNHASSCCGKSR
                     QPS"
BASE COUNT          511 a          862 c          875 g          452 t
ORIGIN      
        1 gcgcacgctc gtcggccggc ttgagcccgt catcggcgag gccctcgccg tcggactcct
       61 gacgactgac cagtccaccg acggcggcat caatgcacag cgcgagctgg tggccgcgct
      121 caaggccaag gtcaaggaca acttcgagcc ggaggcgcgc gacctcctat cggtcgcgga
      181 tgcgctggtc aggaagaccg tgtggattgt cggcggcgac gggtgggcct acgacatcgg
      241 cttcggcggc ctcgaccacg tcatcgcctc cggcgcgcgc gtccgcatcc tcgtcctcga
      301 caccgaggtc tactcgaaca cgggtgggca ggcgtcgaag gccactcccc ttggcgcagt
      361 ggcgaagttc gccgccggcg gcaagacgac ggcgaagaaa gacctcggca tgatggcgac
      421 gaagtacggc aacgtctacg tcgcgcaggt ggcgatgggg gcgagcgaca cgcagaccgt
      481 caaggcgatg ctcgaagccg aggcgcacga cggcccctcg cttatcatcg cctacagcca
      541 gtgcatcgcc cacggcatcg acatcgcgaa gggcatgcac caccagaagc tcgcggtcga
      601 gtcgggctac tggccgctct accgctacaa cccggcgctc gccgacgagg gcaggaaccc
      661 tttccagttg gactcgggcg acccgaagat tcccttgcag gactacatct acaccgaggc
      721 gcgctaccgg atgctccagc agagcgaccc agcggtcgcc aagttcctcc tcggccgcgc
      781 gcaggcggcg gtcaacgcga agtgggagca gtacaagcag atggcggaaa ggacgtgata
      841 agtgatgagt gataagtgac aagaaagaag agcgcggatt ttcttatcac ttatcactca
      901 tcacttatca cctatcaaag ctatggactt gagcacaacc tatctgggcc tgccgctgaa
      961 gaacccggtc gtggcgtcgg cctcgccgct gtcaagcact ctcgaaggca tccggcgtct
     1021 ggaggacgcg ggcaccgcgg ccgtcgtcat gcactcactc ttcgaggagc agatttacct
     1081 tgaaagtgtg tccctgagcg accaactcga ccgcggctcc tactcgaccg ccgaggcgct
     1141 caactacttc ccggagatgc agtcttacgg cgtcggcccc gaaaaatatc tggagctgat
     1201 acacgcggcc aagctcaccg tgggcgtgcc gatcatcggc agcctcaacg gcacgacgcc
     1261 gggcggctgg atcgagtacg cgcggctcat cgaggacgcg ggcgcggacg cccttgagtt
     1321 gaacgtctac ttcatcccgg ccaaccctta cgtcacggga gaggcggtcg agcgtcgcta
     1381 cctcgacatc ctgcgcgacg tgaagcgcac ggtctcgatc cccgtcgcca tgaagctcag
     1441 cccgtacttc agctccatcg gcgacatgtc gcggcggctc gtggacgagg gcgccgacgc
     1501 gctcgtcttc ttcaaccgct tctaccagcc cgacttcgat ctggagaagc tggaggtcga
     1561 gccgcgcctc atcctctcgc gctcgcacga gctgcgcctg ccgctctgct gggtggggat
     1621 tctctacggc gagctgctga cggacttcgc catcacgagc ggcgtgcaca cctacgagga
     1681 cgttttgaag tcgctgatgg cgggcgcgaa ggtggcgatg atggcctcgg agcttttgca
     1741 gaacggcgtg gggcgcgtcc ccgtcatcct cggacagatg agtgagtgga tggagcgcaa
     1801 cgagtacgag tcggtcaggc agatgatcgg cagcatgagc cgcacgaacg tcgccgaccc
     1861 ggctgccttc gagcgggcga actacatgaa gatgctggcc tcgtaccaga gaaacagagg
     1921 ttagaggcca gaggtcagag gtcagtgaaa gacttgctct taactaacct ctgacctctt
     1981 tcaccggagg tgaattatgt cgaaggacga acgcgacgtg ctcgaactgt taaaggcgga
     2041 gctggattac attgagtccg gcggctacgg gcgctcggtg cgaacgccgt ggcggccgac
     2101 ctccgtcttc caggattcgc tgacctgcat caacttcggc tacccgtacc gcgcgcaccc
     2161 ctgcggggag tgcttcctcg acgcgctcgt gccgcccgag agccgcaccg ccgaggttcc
     2221 gtgtcatcac atcccgctcg acgccgaggg gacgaccgtc gaggcggtcg agtcggaggg
     2281 gaaccaggcg atgctcgaag agaaggttaa ggcgtggctg cgcgcgaaga tcagggagat
     2341 cgagggcgag cgcgccctcg ctgtcgagag ataagaaaga gcttatgcgc ctcgcacacc
     2401 caagtctgag cagcttcacg cgccgcctgg cggtcgtggc cttcggcctc tggctcggcg
     2461 gcgccgggtg cgctttctgc tgcgcgtcgg ccgaaaaccc cgcgcgcgtg caggatgcta
     2521 cctcatctgc gggcgcgcag gcggatagta cgatttttgc gccgacgcac tgcccggcgc
     2581 acgcgcgggc gtcggcgggc acgactgacg gttttgaacg ggatgctact tccgtacaga
     2641 cgccttcgcc gacggacaat cacgcaagct cgtgttgcgg gaagtcgcgt cagccttcag
//
LOCUS       DASVES010000298         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_988307, whole genome shotgun sequence.
ACCESSION   DASVES010000298 DASVES010000000
VERSION     DASVES010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988307"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VF240_12015"
     CDS             complement(<1..554)
                     /locus_tag="VF240_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7175976.1"
                     /translation="MNSLGFPATSPDVSFTLILPELIVSVAGVLVMLVDAFTRRRDER
                     WITGTISLAGLAAAAAACFWLWDAPGLRREAFNGMVVLDSMRLSFTLVFILIAALTVL
                     VSMIWMEWERLPAGEFHTLLLFATVGMMLMASGGDLVVIFLGLELLSIATYVMAGFRK
                     TDLRSNESSMKYFILGSFSSAFLL"
     gene            complement(564..1181)
                     /locus_tag="VF240_12020"
     CDS             complement(564..1181)
                     /locus_tag="VF240_12020"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7175977.1"
                     /translation="MKFKLAILFTFCLALTSLDARAQTTAAATADDEPAKSLGIKLKA
                     LDGKTYDTASMPGEVVVASFGATWCAPCIWELKAIEELMEEYNGKPVRFLWISIEDER
                     RTPDGVLKKFAKQYRLTLPVMRDDGVTFAQFSDNRRIPFVIFFDTEGRFAAPAHRGMA
                     SEPIQYKQMVRRRVDALLAAAAQEPKAAGENKAAEGRTGAPPSQD"
     gene            complement(1212..2795)
                     /locus_tag="VF240_12025"
     CDS             complement(1212..2795)
                     /locus_tag="VF240_12025"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7175978.1"
                     /translation="MRDWLLTILILLPALGALAVAAHRLAPYRRETHYRWIALAFSVV
                     TFALSLLLLDGSSGAEAGAFRFVRDVPWIEAIGARYHVGVDGISLWLVILTTLLVPIS
                     VLSSWTAVEKRPLAFFMFMLLLESAMIGVFVSLDLLLFYLFFEASLVPMFFLIGVWGG
                     ERRIYAAVKFFIYTAVGSLLMLAGILALYFLSASPETGVAGTFDYVRLLEAMQSGALV
                     LSPRAAYWLFLAFALAFAIKVPLFPFHTWLPDAHTEAPTAGSVILAGVLLKMGTYGLM
                     RFNFTLFPEMSREFAPVMITLAVVGIIYGALVAMVQPDVKRLVAYSSVSHMGFVILGM
                     FSFTEAGMQGALYQMLNHGVSTGALFLLVGMIYERRHTRQISDFGGLSKPMPWFATLF
                     VFASLSSVGLPLLNGFVGEFLIMLGMWTSDASIHNVARTWVVTMLAATGVIWAAVYML
                     WMLQRVLFGKVTNEKNARLSDLNWRELGLLAPLLALMIYMGAYPAPFLNRSRASVDMV
                     RARVVQTGTFHAEGGDQQR"
     gene            complement(2802..>4816)
                     /gene="nuoL"
                     /locus_tag="VF240_12030"
     CDS             complement(2802..>4816)
                     /gene="nuoL"
                     /locus_tag="VF240_12030"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7175979.1"
                     /translation="LADNLLLMFVGWEGVGLCSYLLIGYYFDRKEAGDAAKKAFVANR
                     VGDWGVVLGIMLVFWLTGSVSFFAKSAAVTGGEPVASALASVAAMPVDPFTWGAIVAG
                     GITSAAVLLFIGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVVRCSA
                     IYAHAPTAMFIMAIIGAATAIFAATIGLAQNDIKKVLAYSTVSQLGYMFLACGVGAFV
                     AAIFHVMTHAFFKALLFLGSGSVIHGMHHEQDMRRMGGLRKYMPITFATMLTGWLAIS
                     GFPLFSGFFSKDEILWKTWSTEALGIPAGAGKFLWIVGAVTALLTAVYMTRLMWMTFF
                     GEERFRETHAGGQADEAHAHAHDEGHKPHDARDPAAGDRPLHEPGERVGHAAAIADHT
                     HDTPHTHGAAHAAAVAHDDEEDDAHHHGPVTPHESPWTMTVPLVVLAILSVVGGLVGV
                     PYALSGGAIPNYFEHALEPVVAHAPERGDAAGHGAAQTTTVPPAGATTGGGEHGVEML
                     SPAPQPHDGASPISVGEGQVGAPAHEAAHSPEEVAQERLFTGISVVIGLVGIALGILI
                     FGKRPLLALPRLLENKYYVDEAYDAAVINPIEKGSRSVLWRFFDVGVIDGIVNGVGRL
                     ASQAGGVLRYLQPGFVRSYAAIILLGALAVVGFFAYSAYNVLRQMP"
BASE COUNT          938 a         1515 c         1665 g          698 t
ORIGIN      
        1 tagagcagga aggccgagga gaacgagccg aggatgaagt atttcatcga cgactcgttc
       61 gagcgcaaat cggtcttgcg aaaacccgcc atgacgtagg tcgcaatcga gagcagttcc
      121 agcccgagga agatgacgac gaggtcgccg cccgaggcca tcaacatcat gccgacggtg
      181 gcaaagagca gcagcgtgtg gaactcgccg gccggcagcc tctcccactc catccagatc
      241 atcgacacca gcacggtgag cgcggcgatg aggatgaaga cgagcgtgaa cgagagtcgc
      301 atcgagtcga gcacgaccat gccgttgaac gcttcgcgcc ggaggccggg cgcgtcccac
      361 agccagaagc aagcggcggc ggcggcggcg agaccggcga gcgaaatcgt ccccgtgatc
      421 cagcgctcgt cgcggcggcg cgtgaaggcg tcaacgagca tcaccagaac gcccgcgacc
      481 gagacgatca gctcgggcag gatgagcgtg aagctcacgt cgggcgaggt ggccgggaat
      541 ccgagactgt tcatcgctac actttagtcc tgtgaaggcg gcgcgccggt gcgcccctcc
      601 gcggccttgt tttcgcccgc cgccttcggc tcctgcgccg ccgcggcaag gagcgcgtca
      661 acgcgccggc gcaccatctg cttgtactgg atcggctcgg acgccatccc ccggtgcgcg
      721 ggcgcggcaa atcgcccctc cgtgtcgaag aagatgacga acgggatgcg gcggttatcg
      781 gagaactgcg cgaaggtcac gccgtcgtcg cgcatgacgg ggagcgtcag ccggtattgc
      841 ttggcgaact tcttgagcac gccgtcgggc gtgcgccgct cgtcctcgat gctgatccag
      901 aggaagcgca cgggcttgcc gttgtattcc tccatcaact cctcgatggc cttcagctcc
      961 cagatgcacg gcgcgcacca cgtcgcgccg aaggaggcga cgacgacctc gcccggcatc
     1021 gacgcggtgt cgtaggtctt cccgtcgagc gccttgagct tgatgccgag cgacttcgcc
     1081 ggttcgtcgt cggccgtcgc ggcggccgtg gtctgcgcgc gcgcgtcgag gctcgtgagg
     1141 gcgagacaga aggtaaaaag aattgcgagt ttaaatttca tttccggtaa agacggtcat
     1201 cttgaaaacc gtcacctttg ttggtcgccg ccctcggcgt gaaacgtgcc ggtctggacg
     1261 acgcgcgcgc gcaccatgtc cacgctcgcg cgggagcggt tgaggaacgg ggcggggtaa
     1321 gcgcccatgt agatcatcag cgcgagcagc ggggccagca gcccaagctc gcgccagttg
     1381 aggtcggaga gccgcgcgtt cttctcgttc gtcaccttgc cgaagaggac gcgctgcaac
     1441 atccagagca tgtagacggc ggcccagatg acgcccgtcg ccgcgagcat cgtcaccacc
     1501 cacgtgcgcg cgacgttgtg gatggaagcg tccgaagtcc acatccccaa catgatgaga
     1561 aactcgccga cgaagccgtt gaggagcggc aagcccaccg acgagagaga cgcgaagacg
     1621 aacagggtcg cgaaccacgg catcggcttc gacagtccgc cgaagtcgct gatctgccgc
     1681 gtgtgtcgtc gttcgtagat catgccgacg aggaggaaga gggcgccggt cgagacgccg
     1741 tggttgagca tctggtagag cgcgccctgc atccccgcct cggtgaacga gaacatgccg
     1801 aggatgacga agcccatgtg actgacggac gagtaagcga ccaatcgctt cacgtccggc
     1861 tgcaccatcg ccacgagcgc gccgtagatg atgccgacga cggcgagcgt aatcatcacg
     1921 ggcgcgaact cgcgcgacat ctcggggaag agtgtgaagt tgaaacgcat cagcccgtag
     1981 gtgcccatct tgagcaggac gcctgcaagg atgacggagc cggccgtcgg cgcttcggtg
     2041 tgcgcgtccg gcagccacgt gtggaacggg aagagcggca ccttgatggc gaaggccaaa
     2101 gcgaaagcga gaaacagcca gtaggccgcg cgcggactaa gaacgagcgc gcccgactgc
     2161 atcgcttcga gcagccgcac gtagtcgaag gtgccggcga ctcccgtctc gggcgaggcg
     2221 gagaggaagt agagcgcgag gatgccggcg agcatcaaaa ggctccccac ggccgtgtag
     2281 atgaaaaact tcaccgccgc gtagattctc cgctcgccgc cccagacgcc gatgaggaag
     2341 aacatcggca cgagcgacgc ctcgaagaag aggtagaaca aaagcaggtc gagcgagacg
     2401 aacacgccga tcatcgccga ctcaagcagg agcatgaaca tgaagaaggc gagcggtcgc
     2461 ttctccacgg ccgtccaact ggagagcacg gagatgggca ccagcagcgt cgtcaaaatc
     2521 acgagccaca ggctgatgcc gtcaacgccg acgtgatagc gcgcgccgat ggcttcgatc
     2581 cacggcacgt cgcgcacgaa gcgaaatgcg ccggcctctg cgccggaact tccgtcgagc
     2641 agcaggagcg acagcgcgaa ggtgacgacg gagaacgcga gcgcgatcca gcggtagtgc
     2701 gtctcgcgcc ggtagggcgc gagacgatgc gcggcgacgg ccagcgcgcc gagcgcggga
     2761 aggaggatca ggattgtgag cagccagtca cgcatgagtt ttcagggcat ctgccgcagg
     2821 acgttgtagg cgctgtaggc gaaaaagccg acgacggcga gcgcgcccag caagataatc
     2881 gccgcgtagc tgcggacgaa gccgggctga agatagcgga gcaccccgcc ggcctgcgac
     2941 gcgagacggc ccaccccgtt gacgatgccg tcgatgacgc ccacgtcgaa gaaacgccag
     3001 agcaccgagc gcgaaccctt ctcgatgggg ttgatgacgg ccgcgtcgta tgcctcgtca
     3061 acgtagtact tgttctccag cagacgcggc agggcgagca gcggccgctt gccgaagatg
     3121 aggatgccga gcgcgatacc gacaaggccg atgacaaccg agatgccagt gaacaggcgc
     3181 tcctgcgcga cctcctcggg cgagtgcgcc gcctcgtgcg cgggcgcgcc cacttgacct
     3241 tcgccgactg agatgggcga agcgccgtcg tgcggctgcg gcgctggcga gagcatttcg
     3301 acgccgtgct cgccgccgcc ggtagtcgcg ccggccggag gcacagtcgt cgtttgcgcc
     3361 gccccgtgcc cggccgcatc gccacgctcg ggcgcgtggg ccacgacagg ttcaagagcg
     3421 tgctcaaagt aattggggat ggccccgccg ctcagcgcgt agggcacgcc gacgaggccg
     3481 cccactaccg agaggatggc gagcacgacg agcggcacag tcatcgtcca cggcgactcg
     3541 tgcggagtga cggggccgtg atggtgcgcg tcgtcctctt cgtcgtcgtg ggcaacggcg
     3601 gcggcgtgtg ctgcgccgtg tgtgtgcggc gtgtcgtgtg tgtggtctgc tatggcggcg
     3661 gcgtggccga cacgctcacc cggctcgtgg agcgggcggt cgccggccgc cgggtctcgc
     3721 gcgtcgtgcg gcttgtggcc ctcgtcgtgt gcgtgagcgt gtgcctcgtc ggcttgcccg
     3781 cccgcgtgcg tctcgcggaa acgctcctcg ccgaagaagg tcatccacat cagccgcgtc
     3841 atgtagacgg ccgtgaggag cgctgtcacc gcgccgacga tccacaggaa cttgcccgcg
     3901 cccgcgggga tgccgagcgc ctcggtgctc cacgtcttcc agagaatctc gtccttcgag
     3961 aagaagccgg agaagagcgg gaaaccgctg atggcgagcc agcccgtgag catcgtcgcg
     4021 aaggtgatgg gcatgtactt ccgcagtccg cccatccggc gcatgtcctg ctcgtggtgc
     4081 atgccgtgga tgacggagcc ggagccgagg aacaggagcg ccttgaagaa ggcgtgcgtc
     4141 atcacgtgga agatggccgc gacgaacgcg ccgacgccgc aggcgaggaa catgtagccg
     4201 agctgtgaga cggtcgagta ggcgagaact tttttgatgt cgttctgggc gaggccgatg
     4261 gtggcggcga agatggcggt ggccgcgccg atgatggcca tgatgaacat ggcggtgggc
     4321 gcgtgcgcgt agatggccga gcagcgcacc accatataga cgccggccgt gaccatcgtc
     4381 gcggcgtgga tgagcgcgga gaccggagtc ggcccggcca tcgcgtcggg cagccacacg
     4441 tagagcggta tctgcgcgct cttacccgtc gcgccgatga acaacagcac ggccgccgaa
     4501 gttatcccgc ccgcgacaat cgcgccccac gtgaacggat cgacgggcat cgcggcgacg
     4561 ctcgcaagcg cgctcgctac cggctcgccg cccgtcacgg ccgccgactt cgcgaagaag
     4621 ctgacggagc cggtcagcca gaagacgagc atgatgccga gcaccacgcc ccagtcgccg
     4681 acgcggttag cgacgaaagc cttcttggcc gcgtcgccag cctccttgcg gtcgaagtag
     4741 tagccgatga gcaggtagga gcacagtccg acgccctccc agccgacgaa catcaggagc
     4801 aggttgtcgg ccagca
//
LOCUS       DASVES010000299         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_284845, whole genome shotgun sequence.
ACCESSION   DASVES010000299 DASVES010000000
VERSION     DASVES010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284845"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VF240_12035"
     CDS             complement(<1..1330)
                     /locus_tag="VF240_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7175980.1"
                     /translation="MMFLQSIPEKQPALLSLVYVAGFALVGLLLALSLVRNRRRRSAL
                     GSVAAADLPPEVRKRLGVTSTNRGLHAIRWIFVLLAVTIFGFHVYWAREAETQNEQFQ
                     ALSYKDLRNRRLAESTLRGWILDRTGSLDRALAFYRRGERGEILREYPLDVEFAQIFG
                     TDRGDAGLERALFGVESGDTPEAYDVLLERNIKQAANLDVRLTIDSELQRAAAEQLRG
                     RHGAVVAINPQTGEILALYSNPSYSLKAAQDADTWIRLDTNKRDLPLVNRATRSYYIP
                     GSTFKTVTMIAAYVAGMQDTRLTCSGSGYYAAQGAPPIFDDQGAAEVHGNISIDTAYE
                     VSCNQYFAQLAVKLGPQKMEQAARLVGIGAMQTPEQARVARRQPEIMNASSESVAKAI
                     SPFQSTMVTGPKIKTFDLALEGYGQGYAGQMTPFQMALAAATIGNLEGKLM"
     gene            complement(1400..2212)
                     /locus_tag="VF240_12040"
     CDS             complement(1400..2212)
                     /locus_tag="VF240_12040"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7175981.1"
                     /translation="MSPEKEKKRDEALVERLMRKVLESAGSVLDRKLGREVEAPGNFT
                     TSKLIERMKRLFEERARDDGRGGRIAPHLLKLKIEWGTHSEADPEAIKELEHEVLAAA
                     IDYINDQRLRTLAPVKVETAVDIFTTGIAVDPTFGEFEDALKQEDEAHRRAGDEIHIP
                     KALTPQDVPYAARITLPDGSQDTVLRFRPGGRRINVGRGSDNDLYLNHASVSKVHAAL
                     MLNREGTLLVADTGSTNGTFINGRRIAYGESRQIEDGDVVGFGDVEVRLKKQ"
BASE COUNT          353 a          691 c          776 g          421 t
ORIGIN      
        1 tcatcagctt gccttcgagg ttgccgatgg tcgcggcggc gagcgccatc tgaaacggcg
       61 tcatctgccc ggcgtagccc tgtccgtagc cttcgagcgc gagatcgaac gtcttgatct
      121 tcggccccgt gaccatcgtc gattgaaacg gcgagatagc cttggccacg gactcgctgc
      181 tggcgttcat gatctcgggc tgacgacggg cgacgcgcgc ctgctccggc gtctgcatcg
      241 cgccgatgcc gacgagacgg gcggcctgct ccatcttctg cgggccgagt ttgacggcga
      301 gctgtgcgaa gtactggtta caagaaactt cgtaggccgt gtcgatgctg atgttgccat
      361 gaacctcggc ggccccctga tcgtcgaaga tgggcggcgc gccctgcgcg gcgtagtagc
      421 cgctgccgct gcacgtaagc cgtgtgtcct gcatcccggc gacgtaggcg gcgatcatcg
      481 tcacggtctt gaaggtcgaa cccggaatgt agtaggagcg cgtggcgcga ttgacgagcg
      541 ggaggtcgcg cttgttggtg tcgaggcgaa tccaggtgtc agcgtcctgc gcggccttga
      601 ggctgtagga cgggttggag tagagcgcga ggatttcgcc ggtctgcggg ttgatggcga
      661 cgacggcgcc gtggcggccg cgcaactgct cggccgcggc cctctgcaac tcgctgtcga
      721 tggtcaggcg cacgtcgagg ttggcggcct gcttgatgtt tcgctcaagc aaaacgtcgt
      781 aggcttcggg cgtgtcgccg ctctcgacgc cgaagagggc gcgctcaaga ccggcgtcgc
      841 cgcggtcggt gccgaaaatc tgtgcgaact ccacgtccaa cgggtactcg cgcaagattt
      901 cgccgcgctc gccgcgtcga taaaacgcga gcgcgcggtc gaggctgccc gtgcggtcga
      961 gaatccagcc gcgcagggtt gactcggcga ggcggcggtt gcgaaggtct ttgtaactga
     1021 gcgcctgaaa ctgctcgttt tgcgtctcgg cctcacgcgc ccagtagacg tggaagccga
     1081 agatggtgac ggcgagcagg acgaaaatcc aacggatagc gtggaggccg cgattggtcg
     1141 aagtcacgcc gagacgctta cgcacctcgg gcggcaagtc ggcggccgcg acggagccga
     1201 gcgccgagcg ccggcgccgg ttacgcacca gcgagagcgc cagcaacaag cccaccagcg
     1261 cgaaccccgc cacgtagacg aggctcaaca acgccggctg cttctctggg atggactgta
     1321 gaaacatcat aagcgtgata agtgatgagt gataagtgat gagagtaaga aagctgtctt
     1381 tctcatcact tatcactcat cactgctttt tcagcctgac ctccacgtcg ccgaagccga
     1441 ccacgtcgcc gtcctcgatc tggcgcgact cgccgtaggc gatacggcgg ccgttgatga
     1501 aggtgccgtt ggtcgagccg gtgtcggcga cgaggagcgt gccctcgcgg ttgagcatga
     1561 gcgcggcgtg taccttcgag acgctcgcgt ggttcagata gaggtcgttg tcggagccgc
     1621 gtccgacgtt gatgcggcgt ccgccgggtc ggaaccggag gacggtgtcc tgcgagccgt
     1681 cgggcagggt gatgcgcgcg gcgtagggca cgtcctgcgg cgtgagcgcc ttcgggatgt
     1741 ggatttcgtc gccggcccgg cggtgcgcct cgtcttcctg cttgagcgcg tcctcgaact
     1801 cgccgaaggt cggatcgacg gcgatgcccg tggtgaagat gtccacggcg gtctcgacct
     1861 tgacgggcgc gagcgtgcgc aggcgctggt cgttgatgta gtcgatggcg gcggccagca
     1921 cctcgtgctc cagctccttg atggcctcgg ggtcggcctc ggagtgtgtg ccccactcga
     1981 tcttgagctt gaggaggtgc ggggcgatgc ggcccccccg gccgtcgtcg cgggcgcgct
     2041 cctcgaagag acgcttcatc cgctcgatca gcttcgacgt ggtgaagttg cccggcgcct
     2101 caacctcgcg cccgagcttg cggtcgagca cggagccggc gctctccagc accttccgca
     2161 tcagccgctc gacgagcgcc tcgtcgcgct tcttttcctt ctctggactc atatgggcct
     2221 cggccgcgtc accccgttaa a
//
LOCUS       DASVES010000300         5750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_800429, whole genome shotgun sequence.
ACCESSION   DASVES010000300 DASVES010000000
VERSION     DASVES010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800429"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VF240_12045"
     CDS             <1..779
                     /locus_tag="VF240_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7175982.1"
                     /translation="QIENRKLASALAESAEKFYGVHVLSGVRVSGVRVRSGRVEGVET
                     SGGFVAAGAVVLAAGAWTSRIPFLSPEGETLTAHAHPRVVPVRGQMLCFTRPAGGDTD
                     AAAPDATCAMPRHVIYSPRGYVVPRRDDRLLAGSTTEEAGFDKSVTAAGLHAVLTQAL
                     EIVPTLGEFALSDSWAGLRPRADDALPVIGESPEVERLFYATGHYRNGILLAPLTARI
                     VSRMVVGGAFDAPDAWPQQYRVEVFSPARLLPAHASTRQV"
     gene            788..2200
                     /locus_tag="VF240_12050"
     CDS             788..2200
                     /locus_tag="VF240_12050"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7175983.1"
                     /translation="MARLFRLASVTLHAPPAVATLHFALLLFLFCAVAPPLASAQDER
                     QGSATSASPPAAAAVESEAARLYAEASQYKNKKFEEFIKTGVPYNKELEEQTLREQRE
                     LALRHADRLAAASTSARGLDLYYLGMLYVLGERHAAAFDALNRLLGPEGAGAPAQTLD
                     DARGIFIQQALKLKRAGEAERVASEYARAAQPKPLARYRYETMLTAHYRETRDFARAA
                     PHAREAYGAALLLAGERSIEPGRRDKLLFGASKALADTLSQAGRQAEAVQMLRELRAV
                     AHALPSARLYLDAVGMLEDYGDAQEADGATELAVAADSAASAVRPASPEIVVDDWIEQ
                     TPVSLAALRGRVVLLDFWATWCGPCRETIPRLSALQRKYGARGLTVLGLATYQGKGEG
                     RPMTPPQELAYLRQYKKRVGASYGFAVTAAETNEDNYGVNSFPTAVLLDRRGRVRLIS
                     IGAGEERLLEATVRKLLEEK"
     gene            2394..3662
                     /locus_tag="VF240_12055"
     CDS             2394..3662
                     /locus_tag="VF240_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX7175984.1"
                     /translation="MEGSNITIAIAFAAGLLSFLSPCVLPLVPGYLSLISGVSVEHLK
                     GGGGSRARARKAVILNSLAFNAGLSIIFIALGAAAGWVGSAIISNPWVRIIGGLVIIA
                     FGLQLIGLLKIGALYRDTRKFSDEKPRGPLGALTLGMAFAAGWTPCIGPILGGIIGLA
                     ATSGQWQSGLVLSAFYAAGLAVPFLVTGVLLNQFLGFYSRFRRHLHKVEVISGVLLIV
                     IGVMVMSNSVTWLASVASGVIPNAEGWLQSKVESSQTAQAPAAPAAATGQSAQPAAAS
                     KANFEPAPDVELKTLDGQPLRLSDLRGQVVLLNFWATWCVPCRAEIPELNAMHNELGA
                     RGFTVLGVTTHDTAALVREYQKDVRQDYRVVTGDQSVASKYAAVGLPTTFIIDREGRI
                     RQRIVGERKRPAFDAVVLPLLEEAAATASN"
     gene            3740..4528
                     /locus_tag="VF240_12060"
     CDS             3740..4528
                     /locus_tag="VF240_12060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX7175985.1"
                     /translation="MSRLRTITFAAAFALVACAHAAAQQHQHQQPAQPAASPQATQPA
                     QPAPSTQPTQAAQPASSPQPAQPAATPNPNAGHEYAPLLEKEINYKDWTFKSLKDGTS
                     VNLREWAKGKKLVMVVYFAPWCGNWKFEAPIVSKLYDKYKQHGFDVIAVSEYATADDA
                     RKYFEKAGLSSFPVVVESEAREDRDKTTHYGYRQACADTRKWGSPFNVFLEPAKLKWS
                     GDVVTEKAWVVSGELIEKDAEQFIREKLGLSEAASLAPCENKKQ"
     gene            4718..5677
                     /locus_tag="VF240_12065"
     CDS             4718..5677
                     /locus_tag="VF240_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7175986.1"
                     /translation="MKLRLLYHGNCFDGAASAAIFTRFYRERVRPDAEIVYGGLLHRA
                     GSLFDPEMFDGDENAIVDFKYSPSERLTWWFDHHQSAFLTPEDEAHFRADASGQKFWD
                     AASKSCTEFIARVARDHFGFDAPELAPLVHWAHVIDGAEYESPAQPVELKEPALRLMQ
                     VIENAEEEFVVYIIGLLAGRTLEEAAGDAEVQRRLQPILARHRETIETVRRKIKYASG
                     VVNFDLVDEGRDGFNKFIPYYLHPETTYSVAVTRGSQRAKISVGSNPFAPRPRRHNIA
                     TICERYGGGGHAVVGAVSFKPDEVERAREVAREIVAELSEGFE"
BASE COUNT          952 a         2028 c         1834 g          936 t
ORIGIN      
        1 ggcagatcga gaatcgaaag ctcgcgtcgg cgctcgccga gtcggcggag aagttctacg
       61 gggtgcatgt gctctcaggc gtgagggtgt cgggcgtgcg cgtccgcagc gggcgcgtcg
      121 agggcgtcga gacctccggg ggctttgtcg cggcgggcgc ggtcgtcctc gcggcgggcg
      181 cgtggacttc gcggattccg ttcctgtcac cggagggcga aacgctgacg gcgcacgcgc
      241 acccgcgcgt cgtgcccgtg cgcggacaga tgctctgctt cacgcgtccg gccggcggag
      301 acaccgacgc cgccgcaccc gacgcaacat gcgcgatgcc gcgacacgtc atctacagcc
      361 cgcgcggcta cgtcgtccca cgccgcgacg accggctgct cgccggctcg acgaccgagg
      421 aggcgggctt cgacaagagt gttacggccg ccggcctgca cgccgtcctc acgcaagcgc
      481 tggagattgt gcccacgttg ggcgagtttg cgctgtccga ctcgtgggcg ggtctgcggc
      541 cgcgcgcgga cgacgcgctg cccgtcattg gcgaatcgcc ggaggtcgaa cgactctttt
      601 acgccacggg tcactatcgc aacggcattc tgctcgcgcc cttgacagcc cgcatcgtct
      661 cccgcatggt tgtcggcggt gcgttcgacg cgcccgacgc gtggccgcag cagtaccgcg
      721 tcgaagtctt ctctcccgcg cgtctgctcc ccgcgcacgc aagcacccga caggtatgag
      781 aactttcatg gcaagacttt tccgtctcgc gtccgtaact ttacacgcgc cgcccgcggt
      841 cgcgacactc cacttcgcac tcctcctctt cttgttctgc gccgtcgctc cgccgctcgc
      901 ctccgcgcag gacgagcgac agggcagcgc gacttcggcg tcgccgcccg ccgctgcggc
      961 tgtagagtcc gaggccgcga ggctctacgc ggaggcgtcg cagtacaaga acaagaagtt
     1021 tgaagagttc atcaaaacgg gcgtccctta caacaaggag ctggaggagc agacgctcag
     1081 ggagcagcgc gagctggcgc tcagacacgc cgaccgactc gccgccgcga gcacgagcgc
     1141 gcgcggtctc gacctctact atctcggcat gctctacgtc ctcggcgagc gccacgccgc
     1201 cgccttcgac gcgctcaacc gtctcctcgg ccccgagggc gcgggggcgc cggcgcagac
     1261 tttagacgac gcgcgcggca tcttcattca gcaggcgctc aagttgaaac gggccggaga
     1321 ggccgagcgc gtcgcgtccg agtacgcgcg cgccgcacag cccaagccgc tcgcccgcta
     1381 tcgctacgag accatgctca cggcgcacta ccgcgagacg agggacttcg cgcgcgccgc
     1441 cccgcacgcc cgcgaggctt acggcgcggc cctgctcctc gccggggaga ggtcaatcga
     1501 gccgggccga cgcgacaagc tgctcttcgg cgcaagtaag gcgctggccg acacgctctc
     1561 gcaagccggc cggcaagcgg aggccgtcca gatgttgcgc gagctgcgcg ccgtcgccca
     1621 cgcgctgccc tcggcgcgtc tctaccttga cgccgtgggg atgctcgaag actacggcga
     1681 cgcgcaggag gcggacggcg caaccgagtt ggcagtcgcg gcggacagcg cggcgtcggc
     1741 cgtgcgtccc gcgtcgcccg agatcgtcgt cgatgattgg atcgagcaga cgccggtgag
     1801 cctcgcggcc ctgcgcggcc gggtcgtgct gctcgacttc tgggccactt ggtgcgggcc
     1861 gtgccgcgag accatcccgc gcctgagcgc gttgcaacgc aagtacggcg cgcgcggcct
     1921 cacggtgctg gggctggcga cctatcaggg caagggcgaa gggcggccga tgacgccgcc
     1981 gcaggaactc gcctacctcc gacagtacaa aaagcgcgtc ggcgcctcct acggcttcgc
     2041 cgtcaccgcg gcggagacga acgaggacaa ctacggcgtc aactccttcc ccacggccgt
     2101 cctcctcgac cggcgcggcc gcgtccgcct catcagcatc ggcgccggcg aggagcgcct
     2161 gctcgaagcc accgtccgca agctgctcga agagaagtaa aagccgtgaa tcgtcaatcg
     2221 tgaatcgtaa atcgtgagtg gtgaacggaa ggcgagcggt gtccaacttc ctgaatgccc
     2281 cggaagctga agccgggtca accacgattg acgattgacg attcacgatt tacggttttt
     2341 tacacttccg ctttcgcgct ttggggtcgg tttgttgtaa gctctgtgga cgtatggaag
     2401 ggtctaacat cacgattgcc atcgcgttcg cggccgggtt gctgtcgttc ctcagcccgt
     2461 gcgtgctgcc gctcgtgccg ggctacctgt cgctgatttc gggcgtgagc gtcgagcacc
     2521 tgaagggcgg gggcggctcg cgcgcgcggg cgcgtaaggc agtcatcctc aactcgctcg
     2581 ctttcaacgc ggggttgtcg atcatcttca tcgcgctcgg cgcggcggcc gggtgggtcg
     2641 gctccgccat catctcgaat ccgtgggtcc gaatcatcgg cggcctcgtc atcatcgcct
     2701 tcggtctgca actcatcggc cttctgaaaa tcggcgcgct ctaccgcgac acgcgtaagt
     2761 tctccgacga gaagccgcgc ggccccttgg gcgctttgac gctcggcatg gccttcgccg
     2821 ccggctggac gccgtgcatc ggccccatcc tcggcggcat catcggcctc gccgcgacga
     2881 gcgggcagtg gcagagcggg ctggtgctct cggccttcta cgcggcgggc ctcgccgtgc
     2941 cgttcctcgt caccggagtc ctgctcaacc agttcctcgg cttctactcg cgcttccgcc
     3001 ggcacctcca caaggttgaa gtaatctcgg gcgtgctgct catcgtcatc ggcgtgatgg
     3061 tgatgagcaa ctccgtgacg tggctcgcct cggtcgcctc cggcgtcatc ccaaacgccg
     3121 agggttggct ccagtcgaaa gtcgagagta gccagacggc acaagcaccg gccgcgcccg
     3181 ccgccgctac ggggcaatcg gcgcagccgg cggcggcgag caaggccaac ttcgagccgg
     3241 cgcccgacgt ggagctgaag acgctcgacg ggcaaccact tcgcctctcc gatctgcgcg
     3301 ggcaggtcgt gctcctgaac ttctgggcga cgtggtgcgt gccctgccgc gccgagattc
     3361 ccgaactcaa cgccatgcac aacgaactcg gcgcgcgcgg cttcaccgtc ctcggcgtca
     3421 cgacgcacga cacggccgcg ctcgtgcgcg agtatcagaa ggacgtcagg caagactacc
     3481 gcgtcgtcac cggcgaccag agcgtcgcct cgaagtacgc cgccgttggg ctgccgacga
     3541 cgttcatcat cgaccgcgag ggtcgtatcc gccaacggat cgtcggcgag cgcaagcgcc
     3601 cggccttcga cgccgtcgtc ctcccgctcc tcgaagaagc ggcggcgacc gccagcaatt
     3661 aacgttgacg cagaggctcc cagcgcgaca tagtttcact tcccggcatc tctcccgcag
     3721 tcataaggag acaccagcca tgtccaggct ccgcaccata accttcgccg ccgccttcgc
     3781 cctcgttgcc tgcgcccacg ccgcggccca gcagcatcag catcagcaac cggcgcagcc
     3841 cgccgcctcg ccacaggcca cgcagccggc gcagcccgcc ccttcgacgc agcccacgca
     3901 ggccgcgcag cccgccagtt caccgcagcc cgcgcagccg gccgcgacgc cgaacccgaa
     3961 cgccggccac gagtacgcgc cgcttttgga gaaggagatc aactacaagg actggacgtt
     4021 caagagcctg aaggacggca cgagcgtcaa cctgcgcgag tgggccaagg gcaagaagct
     4081 ggtgatggtc gtctacttcg ccccgtggtg cggcaactgg aagtttgaag cgcccatcgt
     4141 ctcgaagctc tacgacaagt acaagcaaca cggcttcgac gtgatcgccg tcagcgagta
     4201 cgccacggcc gacgacgcgc gcaagtattt cgagaaggcg ggtctgtcgt ccttccccgt
     4261 cgtcgtcgag tcggaagcgc gcgaggatcg cgacaagacg acgcactacg gctaccggca
     4321 ggcgtgcgcc gacacgcgca agtggggctc gcccttcaac gtcttcctcg aaccagcgaa
     4381 gctgaagtgg tcgggcgacg tggtgaccga gaaggcgtgg gtcgtcagcg gcgagctgat
     4441 cgagaaggac gccgagcagt tcatccgcga aaaactcggg ctgtcggagg cggcgagcct
     4501 cgcgccctgc gagaacaaga aacagtaagc agtgagcagt gagcagtaag cagagggcgg
     4561 ccggggtcaa tgtccgaccc ggccgccttc tggcatcagt cgctaccaac gccgccacgc
     4621 cactgcctac tgctcaccgc tcactgctca ctgctcactc tttctgctat gatgtcctgc
     4681 ttttttcacc ctgctttcat agactgttag gctctcgatg aaactgcgtc tgctctacca
     4741 cggcaattgc ttcgacggcg cggcctcggc cgccatcttt acgcgcttct accgcgagcg
     4801 cgtccgcccc gacgccgaga tcgtctacgg cggactgctg catcgcgccg gctcgctctt
     4861 cgacccggag atgttcgacg gcgacgagaa cgccatcgtc gatttcaagt acagtccctc
     4921 cgagcgtctg acgtggtggt tcgaccacca ccagtcggcc ttcctcaccc ccgaagatga
     4981 ggcgcacttc cgcgccgacg cctcgggcca gaagttctgg gatgccgcga gcaagtcctg
     5041 cacggagttc atcgcccgcg tcgcgcgcga ccacttcggc ttcgacgcgc cggagcttgc
     5101 gccgctcgtc cactgggcgc acgtcatcga cggcgccgag tatgaatcgc ccgcgcagcc
     5161 cgtcgagctg aaggagccgg cgctccgact gatgcaggtc atcgaaaacg ccgaggagga
     5221 gttcgtcgtc tacatcatcg gcctgctcgc cgggcgcacg ctcgaagagg cggccggaga
     5281 cgccgaggtg cagcgtcgcc tgcaacccat cctcgcgcgc caccgcgaga ccatcgagac
     5341 cgtccgccgc aagatcaaat acgcgagcgg cgtcgtcaac ttcgacctcg ttgacgaagg
     5401 ccgcgacggc ttcaacaagt tcatccccta ctacctccac ccggagacga cctactcggt
     5461 ggccgtcacc cgcggctcgc agcgcgcgaa aatctccgtc ggctcgaacc ccttcgcccc
     5521 gcgcccgcgc cgccacaata tcgccaccat ctgcgaacgc tacggaggcg gcggccacgc
     5581 cgtcgtcggc gccgtctcgt tcaagcccga cgaggtggag cgcgcccgcg aggtcgcccg
     5641 cgagatcgtc gccgagctgt cggaaggatt tgagtgatat gagaaaactt ttactcgcga
     5701 ccctgattgc cgtttcctgt gtgtcgccgc tggctgtcgt gagcgcgtcc
//
LOCUS       DASVES010000301         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_670602, whole genome shotgun sequence.
ACCESSION   DASVES010000301 DASVES010000000
VERSION     DASVES010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670602"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VF240_12070"
     CDS             <1..437
                     /locus_tag="VF240_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HEX7175987.1"
                     /translation="GARPLFPFGHGLSYTTFRYGNLSIKPLAAGRGAEDSGPRYEVSF
                     DVKNTGAREGADVAQVYVGPTRAKVERPAKELKGFAKVSLRPGESKRVSVVLDGRALS
                     YYDADAKRWHAEPGDYDILVGRSSAQIELRGKLTLTQAAASN"
     gene            484..1230
                     /locus_tag="VF240_12075"
     CDS             484..1230
                     /locus_tag="VF240_12075"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEX7175988.1"
                     /translation="MKRQSTAFILVLMTFSTAVLTTPAAGGQSTQAPVYEVSRTSTPI
                     KVDGKLDDAPWAKAAGAKLVNNSDGSTAVQATEARILYDDKYLYFSFRSADENIWATL
                     RRRDQRLWEEEVVEVFLQADASQPSYIELEVNPLGTMLDIYLLDVRKPLRYESWNSEK
                     LRWAVRVDGSVDGKGGDREWTCELALPLEDVATAARLPPRAGDRWRMNLYRMEARPVA
                     ALLAWSPTRKDDFHLPEMFGWLVFTDRQAP"
     gene            1300..2661
                     /gene="glmM"
                     /locus_tag="VF240_12080"
     CDS             1300..2661
                     /gene="glmM"
                     /locus_tag="VF240_12080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7175989.1"
                     /translation="MRAIPTLKISISGVRGVVGESLTPTLLARFAQAFGTYAGPGAVV
                     IGRDTRTSGEMVRQAVVAGLLSSGCRVVDLGVCPVPTVQLLVRERRARGGIAITASHN
                     PAEWNALKFVNSSGLFLTAAQARQLLDIYHQGEYRKVSGAEMRDVETSAGAIDLHVKA
                     ILDALGPLPEGARRLRVVVDSCNGAGSVVAPRLLEALGVDVVAINVTPDGLFPRGAEP
                     VPENLGALRRAVVESGADIGFAQDMDADRLAVVSEQGEAVGEENTLVLATQYVLGHER
                     GPVVANLSTTHALEVLAARFDCPVYRSRIGEAHVAEEMERRGAVVGGEGNGGVIYPRI
                     NFARDSLVGMALILHLLAETGRTVSEILADLPRTVMLKEKFECRSDRVRAALKRIREE
                     YAHRPQDLRDGVKVTTTDGWFLVRGSNTEPIIRIVAEADTEDGARRMIEEVGGRVRAS
                     LGG"
BASE COUNT          446 a          899 c          993 g          445 t
ORIGIN      
        1 cgggcgcgcg tccgctcttc ccattcgggc acgggctgtc gtacaccacg ttccgctacg
       61 gcaatctttc catcaagccg ttggcggcgg gccggggcgc ggaagactcg gggccgcgct
      121 acgaggtctc gttcgacgtg aagaacacgg gcgcgcgtga gggcgctgac gtggcgcagg
      181 tctacgtcgg gccgacgcgc gcgaaggtcg agcggccggc gaaggagttg aaaggcttcg
      241 cgaaggtgag tctgcgcccc ggcgagtcga agcgcgtatc ggttgttctg gacggccgcg
      301 ctctctcata ttacgacgcc gacgcgaagc gctggcacgc cgagccgggc gactacgaca
      361 tactcgtcgg ccgctcgtcc gcacagatag agcttcgcgg caagttgacg ttgacacagg
      421 ccgccgcgag caactaaagc gtctcgatag gacaggcatt cctgcctgtc tcacgtaatc
      481 tttatgaaga gacaaagcac agcgttcatc ttagtcctga tgacgttctc aaccgccgtc
      541 ttaacgacgc cggccgccgg aggccagtca acgcaagcgc cggtctacga ggtctctcgc
      601 acgagcacgc cgatcaaggt cgatgggaag ctggacgacg cgccgtgggc gaaggccgcc
      661 ggcgcgaagt tggtcaacaa ttccgacggc tcaacggctg tgcaggcgac cgaggcccgg
      721 attctctacg atgacaagta tctgtacttc tccttccgca gcgccgacga gaacatctgg
      781 gccacgctca ggcggagaga ccagcggctc tgggaggagg aggtggtcga agtgttcttg
      841 caggccgatg cgtcgcagcc gagctacatc gagttggagg tgaacccgct cggcacgatg
      901 ctcgacatct atctgctcga cgtgcgtaag ccgctgcgct acgagagctg gaacagcgag
      961 aaactccgtt gggcggtgcg cgtggacggt tcagttgacg ggaagggcgg agacagggag
     1021 tggacgtgtg agttggccct cccgctcgaa gacgtggcga cggcggcgcg tctcccgccg
     1081 cgcgcgggcg accgctggcg catgaacctc taccggatgg aggcgcggcc cgtggcggct
     1141 ctgctggctt ggtcgccgac gcggaaggac gactttcatc tgcccgaaat gttcgggtgg
     1201 ctcgtcttca ccgaccggca ggcgccgtga gcgcgcgggc gcggctcgcc tttcggttcg
     1261 gccgtctgca ctgcaagata ctcgactgag aactgtccta tgcgtgcgat accgacactg
     1321 aagatcagca tctcgggcgt gcgcggcgtc gtcggcgagt cgctgacgcc gacgctgctg
     1381 gcgcgcttcg cgcaggcgtt cggcacatac gcggggccgg gcgccgtcgt catcggccgc
     1441 gacacgcgca cgtcgggcga gatggtgcgg caggcggtcg tcgcggggct gctgtcgagc
     1501 ggctgccgcg tggttgacct cggcgtctgc cccgtcccga ccgtgcagct tcttgtgcgc
     1561 gagcgccggg cgcgcggcgg catcgcgatt acggccagcc acaaccccgc cgagtggaac
     1621 gcgctcaagt tcgtcaactc ttcgggcctc ttcctcaccg ccgcgcaggc ccggcaactc
     1681 ctcgacatct atcatcaggg cgagtaccgg aaagtttcag gcgcggagat gcgcgacgtg
     1741 gagacatcgg ccggggccat cgatctgcac gtcaaggcca ttctcgacgc gctcggcccc
     1801 ctgcccgaag gcgcgcgccg gctgcgcgtc gtggtggact cgtgcaacgg cgccggctcc
     1861 gtcgtcgcgc cgcgactgct ggaggcgctc ggcgtcgatg tcgtcgccat caacgtcacg
     1921 ccggacggac tcttcccgcg cggggccgag ccggtgccgg agaacctcgg cgcgctgcga
     1981 cgggccgtcg ttgagagcgg cgctgatatc gggttcgcgc aggacatgga cgccgaccgc
     2041 ctcgccgtcg tctcggagca gggcgaggcc gtgggggagg agaacacgct ggtgctggcg
     2101 acgcagtacg ttctcggcca cgagcgcggc cccgtcgtcg ccaatctctc gacgacgcac
     2161 gcgctcgaag tgctggccgc acgcttcgac tgccccgtct accgctcccg catcggcgag
     2221 gcccacgtcg cggaggagat ggagcggcgg ggggccgtcg tcggcggcga gggcaacggc
     2281 ggcgtcatct acccgcgcat caacttcgcc cgcgacagcc tcgtcggcat ggcgctcatc
     2341 ctccacctgc ttgccgagac cggccgcacc gtctcggaaa ttctcgccga cctgccgcgc
     2401 acggtcatgc tcaaggagaa gttcgagtgc cgctccgacc gcgtgcgcgc ggcgctcaag
     2461 aggatccgcg aggagtacgc gcaccggccg caggatttgc gcgacggcgt caaggtgacg
     2521 acgacggacg gctggttcct cgtgcgcggc tccaacacgg agccgatcat tcgcatcgtc
     2581 gccgaggccg acacggaaga cggcgcgcgc cggatgatcg aggaggtggg ggggcgcgtg
     2641 cgcgcttctc tgggaggttg aaggcagcgg ccgtttcaga tggagccgac aaaggggcgg
     2701 gaggaagtgt gtcggctgcg cgggcggggc gcgcgtcgtc gttgcgggtt gtcagcgcgc
     2761 gtcgaaaacg tgacaatgcc cct
//
LOCUS       DASVES010000302        28910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1596125, whole genome shotgun sequence.
ACCESSION   DASVES010000302 DASVES010000000
VERSION     DASVES010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 28910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28910
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596125"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /gene="rpoA"
                     /locus_tag="VF240_12085"
     CDS             <1..709
                     /gene="rpoA"
                     /locus_tag="VF240_12085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7175990.1"
                     /translation="DIEHDGEVDVLDPNVYIATVSEGGSLTVEMRLRRGRGYVPAERN
                     FDEDLSLGYIPVDSVHTPVKKVNYAVEAARLGQNTEFDKLTIEVWTDGSVKPDDAIGL
                     AAKLIKDHMSIFINFEEDTDDYAYTNIERPPLPRNDVLDRSVDELELSVRSYNCLKNA
                     NIRTIRDLVQRSEREMLATKNFGKKSLNEIKEILHGMGLDFGMAFDEQGNPIPGSGSS
                     DIDLSDYEDDEDDEDEE"
     gene            813..1520
                     /gene="rplQ"
                     /locus_tag="VF240_12090"
     CDS             813..1520
                     /gene="rplQ"
                     /locus_tag="VF240_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX7175991.1"
                     /translation="MRHLNAHRKLGRTSEHRNSMLRNLVTSLINARDERIVTTLPKAK
                     ELRPFIERVITLSRRAQGLEGDDAVARSLHLRRQAAAFFHTGNQGQTAAAGGRRGQPR
                     PPRTAGVAALKRLFDELGERYKDRPGGYTRIIKLGRRDGDNAELAIIELVDNPREREA
                     AASTSKRAKSVTSGKKKSAKASETKEAAGGRRGGRNAPKAEASGGAGSKETDKAGSGG
                     RRSKKTESTAAEKDTEK"
     gene            1701..2414
                     /gene="accB"
                     /locus_tag="VF240_12095"
     CDS             1701..2414
                     /gene="accB"
                     /locus_tag="VF240_12095"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX7175992.1"
                     /translation="MSEHDETRDAGRAPRAQDETRAQSERPDRDAAARGGAGGGGERR
                     GRRRHGSARPQQQQQGQSPRRNEPSLNMEELRELAELFTSHGLTDFEFENEDIRVRLS
                     RSLGQPAAPPPVAAPAGPAPSTAPASAHASLAAQTSPASDAASGSAATTPAEVKAEEL
                     HIITAPIVGTFYRASSPNVEAFVKAGSHVESATVVCIIEAMKLMNEIQAETTGTVEKI
                     YVENGQPVEFGQPLFGIKK"
     gene            2492..3868
                     /gene="accC"
                     /locus_tag="VF240_12100"
     CDS             2492..3868
                     /gene="accC"
                     /locus_tag="VF240_12100"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7175993.1"
                     /translation="MRRFNKILIANRGEIACRIIWTCKEMGIRTVAVHSTADRDSLHV
                     RFADEAICIGGPTSAQSYLNISAVIAAAEITNVDAIHPGYGFLAESAYFAEICEACNI
                     KFIGPRPDVIRQMGDKVEARRTMTEAGLPVLPGSPEPLRDEGEALAIAREIGYPVIIK
                     ASAGGGGRGMRIVRAEGEFLPAMRTASTEAAAAFKNGDVYIEKFIERPRHVEIQVLAD
                     EHGNCIHLGERDCSIQRRHQKLIEESPSPVLTPEMREQMGHAAVEACRRVGYSSAGTI
                     EFLLDEDQKFYFMEMNTRIQVEHPVTEMVTLVDVVRNQIRIAEGEEISYRQEEIEIRG
                     HSIECRINAEDPATFAPSPGLITTYNIPGGPGVRVDTAVYAGYYVPPFYDSLIAKLIV
                     HARTRELAIARMRRALEVMVIEGVKTTIPLHLAIMDDPHFRSGEYSTKFMEQFFERRN
                     AQGAAQAG"
     gene            3983..4291
                     /gene="rplU"
                     /locus_tag="VF240_12105"
     CDS             3983..4291
                     /gene="rplU"
                     /locus_tag="VF240_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7175994.1"
                     /translation="MAYAIIRSGGKQFRVEEGATVRVPSIDKQAGEQVELEALVVSGD
                     GGIKVGSPAIDGAGVTGTVVDHGRGTKIIVFKKKRRKQYKRRQGHRQDYTTLKIDSIG
                     "
     gene            4390..4695
                     /gene="rpmA"
                     /locus_tag="VF240_12110"
     CDS             4390..4695
                     /gene="rpmA"
                     /locus_tag="VF240_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7175995.1"
                     /translation="MAHKKGVGSSRNGRDSHSQRLGLKKFGGEQVLGGNILARQRGTK
                     WKPGKNVGRGKDDTLFALVDGVVKFEDKGRKGKFISVYPAVAAALSPAPEAPPAVAA"
     gene            4792..5868
                     /gene="obgE"
                     /locus_tag="VF240_12115"
     CDS             4792..5868
                     /gene="obgE"
                     /locus_tag="VF240_12115"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX7175996.1"
                     /translation="MFIDRVKIRVRGGHGGNGVTAFRREKFVPRGGPSGGDGGRGGDV
                     WLEAATSLNTLLHLRYNPEHSAERGRHGEGSNRSGPEGGDILVRVPVGTQVFDPETGD
                     IFCDLAVEGQRWLAAKGGRGGFGNSHFATSTNRAPRYHQQGSPGEERELQLELKLLAD
                     VGLVGFPNAGKSTLISTVSAAKPKIADYPFTTLEPHLGVVDLGEFRTFVIADIPGLIE
                     GAHQGAGLGDRFLRHVERTKLLLHLVDVSSASGRDPVEDYETINRELAAYDAAMAERP
                     QIVVATKLDALDEPERLEHLRERAARDGREFHAVSSVANTGVRELVYAVGRRLDEIKE
                     SESVIELDAEWSGEGEASTFGRAD"
     gene            5873..6613
                     /gene="nadD"
                     /locus_tag="VF240_12120"
     CDS             5873..6613
                     /gene="nadD"
                     /locus_tag="VF240_12120"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX7175997.1"
                     /translation="MTGARRIALYGGSFDPVHLGHLTVARSLTELFALDEVLFIPAHV
                     APHKRGRRVAPALDRHAMLALATQDEPRFRISTVELDAPERPYTVETLAHFCEAASRD
                     ASRLFFVMGADSWEEITTWREWERVLGMASTIVVTRPGHELEVGHVTPSVRERIVDLR
                     GADVKQAAEEVDADGVKIFFTDAADVDISSTAIRRGAAGRGGALVLENWVAPEVAEYI
                     RKYGLYREVHGTESDDSAGTETAHEQEG"
     gene            6555..7100
                     /gene="rsfS"
                     /locus_tag="VF240_12125"
     CDS             6555..7100
                     /gene="rsfS"
                     /locus_tag="VF240_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX7175998.1"
                     /translation="MEQNRTTAPERKRRTNKKAEEAVETQEATAADATPLAPEAAEEG
                     LDARMLAALHAAGDKKAHDLLALDLRPVASFTDFFLIGSAQNTRQVQAIADEIVERLR
                     AMGSRAARVEGYTSAEWVLLDFGDFVFHIFEQKARRFYELERLWRDAARVALPPELSA
                     AAAADDARAGSDVEGSLRSEQ"
     gene            7097..8092
                     /locus_tag="VF240_12130"
     CDS             7097..8092
                     /locus_tag="VF240_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7175999.1"
                     /translation="MIEIVARSGAMREALRLARRVAPTNANVLVTGESGAGKDALAQF
                     VHEHSTRAARDLVKIDCASLPAGLLEAELFGYERGAFTGAIAAKPGRLEAADGGTLVL
                     DEVAHLSTDAQAKLLRVIERREFERLGGRRTVRVDARLVALTNVDLDEAVRRRAFRED
                     LFYRLNVVRIDLPPLRERREDVPALARQFLEKYAAKHGRRLKSLTAEAKSLLAGYEFP
                     GNVRELANVVERAVIVAEGGRVEASDLPESVRAAVRVSSRRTMRPTLAEVEDEYIHET
                     LAAAGGNKTEAARVLGISRKNLYERLARSGNKEVGGQRSEVSKRNADADETTTDL"
     gene            8117..8578
                     /locus_tag="VF240_12135"
     CDS             8117..8578
                     /locus_tag="VF240_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX7176000.1"
                     /translation="MRLRLIWVGKTRDEHLRALVGEYLKRLGRYAKCEVAEVREGGSA
                     DERTCLEEEGKRITASLASDAVTVLLDVGGREYSSHELAAQVEVWQGRSVKEVAFVVG
                     GHLGVSDDVRRRADIRWSLSRLTLTHEMARVVLVEQLYRAYTILRGVPYQK"
     gene            8777..9145
                     /locus_tag="VF240_12140"
     CDS             8777..9145
                     /locus_tag="VF240_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX7176001.1"
                     /translation="MDKKKMKTYRDRLLERRESLVSQVTEAELSSRERDMEATQDPAD
                     MAANAYTKELLISMSANDRALLGLIDEALARVESGDYGECVNCGELILEKRLNAVPWA
                     RYCLRCQDMKEQGLLNEEEE"
     gene            9168..10058
                     /locus_tag="VF240_12145"
     CDS             9168..10058
                     /locus_tag="VF240_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7176002.1"
                     /translation="MKAPRAGSFRRAGLFVYALPNAPELSSDTRAVPCRPRRAASTND
                     LTMRIPARLDRLCDAALALFYPLACAACGAEGVEARADFPVCAACWARTRVLTGDETV
                     CWKCGTRARAEVDASSRETVRCRRCDSWAFTAARACGVYEGALRAAVLTLKHEPRVGG
                     RLARLMWEAQRRPPLDCSTRVVPVPLHPERERERGFNQAAVLARAVARRAGLPCDEQS
                     LVRITHTEQHRAGMDARARRETVEKAYRVARPRLVRDEKILLIDDVLTTGATVSSCAA
                     ALRAAGAQEVYVLTVARAGA"
     gene            complement(10163..10582)
                     /locus_tag="VF240_12150"
     CDS             complement(10163..10582)
                     /locus_tag="VF240_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176003.1"
                     /translation="MDDKTILCPSARCEEGAVLVGIVMSDGRVAFASDRIVIDREFVQ
                     IAHEGRPPEKRFRFSSPCAKGGCSQWTGERCGVIDEELAAAPRAAPGELPECSIRPQC
                     RWFEQSGAAACAVCPEVITDMRVDIEVNPASAVEALA"
     gene            complement(10605..10886)
                     /locus_tag="VF240_12155"
     CDS             complement(10605..10886)
                     /locus_tag="VF240_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176004.1"
                     /translation="MAKGEFIVKLDGLDLSTDAEDRIAAAIRAAVMQELAKIDTGGKK
                     AGAGKAGAGGGSGFAAMIHPEWRGMIMARFAKDLFNKGIPNLGVREVRM"
     gene            11175..12128
                     /gene="pip"
                     /locus_tag="VF240_12160"
     CDS             11175..12128
                     /gene="pip"
                     /locus_tag="VF240_12160"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX7176005.1"
                     /translation="MRTFYPPVEPYDAGMLAVSPVHTLYYEQSGNPDGLPVVFLHGGP
                     GGGTVPVYRQFFDPEAYRIVLFDQRGSGRSTPHANLEENTTWHLVADIERLREHLGIE
                     NWVVFGGSWGSTLALAYAQTHPARARALVLRGIFLCRRKEIRWFYQEGASAIFPDVWE
                     EYVRVIPEPERDDFLSAYHRRLTSDDEAVRLEAARAWSVWEGSTSKLFPDSSLIEHFG
                     EPHMALALARIECHYFVNDAFFETENQLVENVGKIRHIPAVIVQGRYDVVCPMMSAWE
                     LHRAWPEADLRVIADAGHSATEPGIVSALVEATDRFKNGDK"
     gene            12246..12776
                     /locus_tag="VF240_12165"
     CDS             12246..12776
                     /locus_tag="VF240_12165"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7176006.1"
                     /translation="MNGRVLVLNASYEPINVCTERRAVVMIFKGVARMEEHNGHMLHS
                     ARITMHAPSVIRLTEYIHIPFKNRTLSRKNILLRDHSTCQYCGRQLPPSELTLDHIHP
                     RSRGGESSWDNLVACCKACNHRKGSRTPEEARMHLIRRPRGFSLHVTRQIMRFMGRAD
                     RTWRKYLFYESDNGEV"
     gene            12825..13100
                     /locus_tag="VF240_12170"
     CDS             12825..13100
                     /locus_tag="VF240_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX7176007.1"
                     /translation="MMTSLLFGMPQGAEWLIILFVVLLIFGSTRLPQLARGMGKSIKE
                     FKKGINESGDDDEVEETRRARELRAGETARTREDELAPDKLSANKPR"
     gene            complement(13187..14014)
                     /locus_tag="VF240_12175"
     CDS             complement(13187..14014)
                     /locus_tag="VF240_12175"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7176008.1"
                     /translation="MTSGKTEKELAFLHDLYVATDWGERFAGLIDEHVQLPKKGRVLY
                     VGAGTGEHALALKARAGREVELVCVDESEERLALARAKAAATPAASDTVFSHEQLDAL
                     SLPDETFELVVGDASMLAPERLPEMLAEMTRVAAPGGAVALDVASASSFGEFFSVYWE
                     ALASAGFDEQAAGVGALIDELPTVSDLEGLAAREGLDDVRSWTNVEEFNYPTGAEFLN
                     APLVRDFLLGRWLESLPEDDARTRVLGEVERIIDEERNDMDYTLSIKTTLILGRRAE"
     gene            complement(14161..15471)
                     /locus_tag="VF240_12180"
     CDS             complement(14161..15471)
                     /locus_tag="VF240_12180"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7176009.1"
                     /translation="MPRSTNESLFAEPEEDTAPSDALAPLAERMRPRTLDEFVGQQQL
                     IGEGRVLRRLLEAGGKLPSLIFWGGPGTGKTTLARILARHTDAHFIQLSAVFSGVRDL
                     RAAIAEARASRRTGRRTVLFVDEIHRFNKAQQDALLPAVEDGTVTLIGATTENPSFEV
                     IGALLSRSRVLTLQPLEGLDVETLLRRALADEERGLGRLKAVVADELLARLASAAGGD
                     ARVALSALEAAVESTPPDKKGARNVAEETVAESLGRARYGYDKGGEEHYNLASALIKS
                     LRNSDVNAALYWLARLIEGGADPIFIARRLCILASEDVGLADPQAMVQAASAAKITEL
                     IGLPEALYPLSQATVYLARAPKSNAVKRAYHRALEDAAETAREPVPLHLRNAVTPLMK
                     RVGYGQGYRYVHDDAAAREEMSCLPERLQGKVYWEDDGEVISDE"
     gene            15752..15958
                     /locus_tag="VF240_12185"
     CDS             15752..15958
                     /locus_tag="VF240_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176010.1"
                     /translation="MKDMKDMLRIIGIILLGVFVLAVGVPLVFAAAGIVLGGIIHITV
                     SLIKIAVVLAVAYLILVGVRAVLR"
     gene            complement(16597..16779)
                     /locus_tag="VF240_12190"
     CDS             complement(16597..16779)
                     /locus_tag="VF240_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176011.1"
                     /translation="MAAPALVAPALLAAPALFDAPVFFDAPDFTAAPALFTVLLFRDA
                     PALFFDAPLFFLVAIC"
     gene            16778..17719
                     /locus_tag="VF240_12195"
     CDS             16778..17719
                     /locus_tag="VF240_12195"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX7176012.1"
                     /translation="MPVAAAAGDAVPFLRRRDEGPEVDNLQEELCDIGYLTRAQKALG
                     PGKFGPRTEEALKHFQRDNFIAETGVYDEETQAAIRQLNEGVKRGIEGNIVRGLQNRL
                     VSLGFMTQVIAGQGKFGPQTEAALLGFQMAQGIMQTGVLTDETYRGLLAAAPHPTPIT
                     DHGDPTRIDTVLPSEGRGFTTYRRSPGGADQFGRASTIRAITDVAETWSTRHAAPRLQ
                     IGDISRRGGGRLEPHDSHQRGVDVDMRPIRNDGREQPVRFDQAGYSHELTKELCQLIR
                     AKVPGVRIFFNDPRLIGLGLTARLIFHDDHLHVRFPS"
     gene            complement(17873..18745)
                     /locus_tag="VF240_12200"
     CDS             complement(17873..18745)
                     /locus_tag="VF240_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX7176013.1"
                     /translation="MGRQNDFMVRPGSRPRLKNYDTAFTGGFADEDAAGKKIEADCGE
                     LATLQDKLMAEETHALLVILQAMDGSGKDGTIKHVASSLDPQGCTAVNFKKPSDAENK
                     HEYLWRFHREVPERGRITFFNRSYYEEVVAPRVRPEGLGDQNLPTSSKSKDIWKRRMG
                     EINNFEQYLTDNGVVVLKFFLHLSKEKQAERLLERTEEPAKKWKFSAADLENRELWDE
                     HLVAHEEMLARTSTRAAPWHVVPADHRWFSALAVADLMVRKLRSLKPRYPAVNGEEKK
                     EMEKARRRLARELR"
     gene            complement(18870..19970)
                     /gene="ychF"
                     /locus_tag="VF240_12205"
     CDS             complement(18870..19970)
                     /gene="ychF"
                     /locus_tag="VF240_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX7176014.1"
                     /translation="MLRAGIAGLAYVGKTTLFNALTSQQSALAASYPFASNKSNVGIV
                     PVPDERLEPLARLVKTSVIKPAVIEFVDIPGLVRGSSKGEGLGNQFLANIRESDTVVQ
                     VVRCFESEEVVHVEGSVNPRRDIETVQIELALADLTSVERRRERTQRTAKSGDKQAVA
                     ELGVLDKLQPALEEFRPARSVALSDDELALARNFFLLTLKPNIYAANVDEATLSDPDA
                     SAHVRAVREVAAREGAEAVVICAQLEAELVALSPEERREYLQSLGVESSGVDSLIKSA
                     YHLLGLMSFLTAGEMEVRAWTIPQGTRAPQAAGAIHSDIERGFIRAEVVGYEDLLSAG
                     SRAAAREKGLTRLEGKEYVMREGDVVDFRFNV"
     gene            20196..22364
                     /locus_tag="VF240_12210"
     CDS             20196..22364
                     /locus_tag="VF240_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176015.1"
                     /translation="MPFAILLALAAVSGGTLLTYLYDREASPLVRVCEGLCTGFAALG
                     LVGFIIASFAGLQPAALVAAGLFVASPLILLRKAEWRARVRVDAREASRTLGRAITFR
                     SGGATFVFYAAAAVLCWLVFRQAMYAGADGIFTGLDNNIGDLPFHVGIISGFVHGDNF
                     PPEHPEFAGTRLTYPFLADFLTAMFVRAGASLEGAMFWQNFTLALSFVALLGRWAALV
                     TGERAAGFLAPALVLFNGGLGFLQVFGEAARGGGLLHTLGNPTVYYTMMGDTYKWGNF
                     VTMLLVPQRGLLLGFPLAIIVLTLWWQATSGEKGEGVKGKGEEGRGKREHREAATRKG
                     EATKKGKRNVSSSDARAKSSAAAAQLTHSTPFPLTLSPFPFRQMLAAGVIAGLMPLVH
                     AHSFVVLMGMGGCLALLLSWRGARDAVARGEVGKEAGAGWRGWFVFFAAATVVAAPQM
                     LWATYRSDVQAGSFFGWQLGWDRGTLNPIVFWLKNTGLFIPLLVAALAWRGQKPLVSR
                     RLLVFYLPFTLCFVVPNLLKMSPWIWDNIKILFYWWVASAPIVALLIVRLWRRGGAWR
                     AAAAVSVVVLTLAGALDVWRVASGGMRQPVFTREGVEFAEMLKRETPPRSLVLHAPTY
                     NHPVYLTGRRSLMGYAGHLGSHGIDYTERERDFARIFSGGPEAEALLKQYGVDYVVVG
                     PLERRGGEMARIRAVVNETFFQRYTKVGQTGGYSLYKTSP"
     gene            22333..24267
                     /locus_tag="VF240_12215"
     CDS             22333..24267
                     /locus_tag="VF240_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEX7176016.1"
                     /translation="MATASTKRRPKKGGAGAKRAAAAAERAQGWPERERVEVSERVWM
                     AASLVILAAAVFLRLYALELRPMHHDEAVNGFFLTGLFREGRYSYDPTNYHGPTIYYF
                     TLPSVYLFGLKTFAVRLVPVFFGVGVVWLALCLRRWVGPLGALTAAALLAVSPGTVFY
                     SRYFIHEMMFLFFALGAVVGVLRFYDTGRGEYLLLASAALALHFATKETAFMTAGVMV
                     LATLIAWGYAGYMRGRPHPFAAREVASGRAVEPGLMDVVARLGGWPRLWPLLLASLAL
                     FVAINVLFYSSFFTNSKGVGDALEALKVWSKTGASEFHAKPWHTYLKWLLQEESPLLW
                     LGTVGAALALWSGRNRFAVFAGAWAFGTLAAYSLVPYKTPWLALNFTVPLAIAGGYAV
                     QELAETAAHARSGTQRLFPAAVLSLSLFLLAVGLGFAHVTTPSEYLCRAVGVWCTTPL
                     SAADGPGLSSEWGALVALVLAALVVGWCVWRLYTARKGGAAWRRSAPALALAALAVVV
                     CTYQAWVVSFQQYDNDQYPYVYAHTRRQFISLVGEIERLVARAGTGADTRIAATASGP
                     GSDSYWPLPWYLNDYRSVGYHAQLPADASNLSIIVGNATQDAQLRATLGATHRKVGGT
                     YPLRPGVDLVLYAQRSLTDR"
     gene            24566..28609
                     /gene="smc"
                     /locus_tag="VF240_12220"
     CDS             24566..28609
                     /gene="smc"
                     /locus_tag="VF240_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX7176017.1"
                     /translation="MFKLQKLEITGFKSFADFTELVFTGSGITAVVGPNGCGKSNVAD
                     AISWVLGEQRVKMLRGAEMKDVIFQGSRNRLPSGMAEVVLHLVRDETIEEEPDIDDID
                     STLEEIDDQTGAIEDRLAPELFAAAQVPDAEAKLSAPDATAPALLALSEPSAVEVTPG
                     ATSAVDASTVEAPAESVEAPAGLALVPAEGQPEAVTTLEARGDGEETPKPAAQTHHKR
                     HWRPRRMELDFAPGETVTVTRRLYRSGESEYLLNGRVCRLRDIQDLFSGTGLSGAHYA
                     IIEQGRIGQILSAKPMDRRTLIEEAAGITKFRVRQRAAEARLEGARSNLHRVSDIISE
                     IDRQVNSLRRQAGKARRYSALRDELRDLLRHTYAAEDRALGTLLETLRAQITDASEEA
                     DALSSKLSAGEDEARRATSEARTREEELAETRAAVAESTLRQDRRERERAYQEEQADA
                     LHKRAAEVRQEIDTLRGRLSAIEQEGEGLRLSDAQLREESDASAGLLASAEAAYAQRL
                     AEVAEAEAVIEVARAELLTHTAVVERLREIGRQLEAALDRVAMQAEGLAREGERAAVA
                     HEQSQAQAQILNEQIRVARAQLESLVAEREESARLIAEARVRVAATSERHARVRDEAA
                     RVRHRLDTLEEIDTQRSLYPQAVQRIFSDAAEGEAIRDFRAIGTLADVLRVEPHWEPA
                     VESVFGTLLQSVIVPTPDDALRAAAWLEVAGAGRANFLVAGLHGGSESDEEQVEQIEQ
                     LGQAETEQPRQSLAESVLAEGVELRAWNLLGAPREIAAVLRRTVPREMNARVVRDLEG
                     AVELSLTSEEMCVTPAGEWVIGGQLVAAGGPPVAGEGAGLLSFKREMRDLGARAVGLE
                     ADLIFAEEAATAERTRLGELEDAYVHLNEAIGRDEREQVAREVNAAQLTQETERAERH
                     MRVVADDAARLADEQRELQERRDQQSAETESVEAARVAATLAIAVAADTLAAARRTSE
                     EASELLGQQRAAAAAASERRRAAASELKRMESESADLAARLERYESEVSEMHARLERL
                     RGSLAEMDEGAGEVEGERERLELDVEEAARRVAEARARADALALEVTELHQSVAAARD
                     ARAGLEVQRAETGARLSYVRESCMAELSAPLEEVAREFAPGEEFDLESERARVEEMRA
                     RLESFGAVNMMALEELGENEERLKFLTEQRADITDGIISTEEALREIKRRSRERFRHA
                     FEEINRNFGQFFLELFGGGRGEMSMIDADDVLESGIDIVAQPPGKRLQNVLLLSGGEK
                     AMAALALVLAVFRYRPSPFCLLDEVDAPLDEANVGRFTTKIKEMSQETQFIVVTHNKR
                     TMEMARALYGVTMEEAGISRLVSVRFE"
     gene            28606..>28910
                     /locus_tag="VF240_12225"
     CDS             28606..>28910
                     /locus_tag="VF240_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176018.1"
                     /translation="MSMWVKVLGFTFAAALVSTGAAFPVQAQSPRKATPRPTPTPAVE
                     QQQPKKVSQRPEDGKGTEEAPASVRAGVPAKPKEDPNAAHYVYEFKQPDFFVYFVRI"
BASE COUNT         4977 a         9498 c         9690 g         4745 t
ORIGIN      
        1 cgacatcgag cacgacggcg aagtcgatgt gctcgatccc aacgtctaca tcgccacggt
       61 cagcgagggc ggctcgctca ccgtcgagat gcgcctgcgg cgcgggcgcg gctacgtccc
      121 cgccgagcgc aacttcgacg aggacttgag cctgggttac atccccgttg acagcgtcca
      181 cacgccggtc aagaaggtca actacgccgt cgaggccgcg cgtctgggtc aaaacaccga
      241 gttcgacaag ctgaccatcg aggtctggac ggacggctcg gtcaaacccg acgacgccat
      301 cggactggcc gcgaagttga ttaaggatca catgtccatc ttcatcaact tcgaggaaga
      361 cacggacgac tacgcctaca cgaacatcga gcgcccgccg ctgccccgca acgacgtgct
      421 cgaccgctcc gtggacgagc tggagctttc cgtgcgctcc tacaactgcc tgaagaacgc
      481 caacatccgc accatccgcg acctcgtcca gaggagcgaa cgcgagatgc tcgcgacgaa
      541 aaacttcggt aagaagtctc tcaacgagat caaggaaatc ctccacggca tgggcctcga
      601 cttcggcatg gccttcgatg agcagggcaa ccccatcccc ggctccggca gcagcgacat
      661 cgacctcagc gactacgagg acgacgagga cgacgaggac gaggagtaaa gcggccgtta
      721 gcgattagct gttagctgtt agcttaaaga gaacttcttc gctctaagct aaatgctaaa
      781 agccaatctc taacagctcc tgagattttg ccatgcgaca cttaaacgca caccgaaaac
      841 tgggacgcac gagcgagcac cgtaattcga tgctccgcaa cctcgtgacc tcgctcatca
      901 acgcgcgcga cgagcgcatc gtcaccactc tccccaaggc caaggagctg cgccccttca
      961 tcgagcgcgt catcacgctg tcgcgccgcg cacagggact cgaaggcgac gacgccgtgg
     1021 cccgctcgct gcacctgcgc cggcaggccg ccgccttctt ccacacgggc aatcagggcc
     1081 aaacggccgc cgccggcgga cgccgcggcc aaccgcgccc gccgcgcacg gccggcgtcg
     1141 ccgcgctcaa gcgcctgttc gacgagttgg gcgaacgtta caaggatcgg cccggcggct
     1201 acacacgcat catcaagctc ggccgacgcg acggcgacaa cgccgaactc gccatcatcg
     1261 agctggtcga taatccgcgc gagcgcgagg ccgccgcgtc cacctcgaag cgcgccaagt
     1321 cggtcacctc gggtaagaag aagtcggcga aggccagcga gacgaaggaa gccgcgggtg
     1381 gcaggcgcgg cggacgcaac gccccgaagg ctgaggcgtc aggcggcgcc ggctcgaagg
     1441 agacggacaa ggcgggcagc ggggggcgtc gctcgaagaa gaccgaatcg acggccgccg
     1501 agaaagacac ggagaagtag accgacgtac ttgaagcgcg ggtcggccgc gcggcgcgtg
     1561 ctccgaaggg gccgcccgcg cccgccccgc ccggcgaccg cagccccaaa ccaccgccgg
     1621 aatcggcggc cggctttggg gcttccactt tgttggaggc gcgcgccaca cgcgcgaacc
     1681 tttgggaggc tcgaacgagc atgagcgaac acgacgagac gcgagacgcc ggacgcgcgc
     1741 cgcgggcgca ggacgaaacc agagcgcagt ctgagcgacc cgaccgggac gccgccgcgc
     1801 gcgggggcgc gggcggcggc ggcgagcgcc gcgggcgcag acgccacggg agcgcccgcc
     1861 cgcagcaaca gcagcaaggt caatcgccgc gacgcaacga gccgtcgctc aacatggagg
     1921 agctgcgcga gttggccgag ttgttcacgt cgcacggcct cacggatttt gaatttgaga
     1981 atgaagacat acgcgtccgc ctgagccgta gtctcggcca gccggccgct ccgccgccag
     2041 tcgcggcccc ggccgggccg gcgccctcca ccgccccggc gtcggcgcac gcctcgcttg
     2101 ccgcgcagac ctccccggcg tcggacgccg cctccggctc tgcggcaaca acgcccgcgg
     2161 aggtgaaagc ggaagaactc cacatcatca ccgcgcccat cgtcggcacg ttctatcgcg
     2221 cgtcttcgcc caacgtcgag gcgttcgtca aggcgggcag ccacgtcgag tccgccacgg
     2281 tcgtctgcat catcgaggcg atgaagctga tgaacgagat tcaggccgag accacgggga
     2341 cggtcgaaaa aatctacgtc gagaacggcc agcccgtcga gttcggccag ccgctgttcg
     2401 ggatcaagaa atgaagaggt cagaggctag aggttagagg tcagttaaaa actgtcttca
     2461 ctagcctctg acctctgacc tctgacctct tatgagaagg ttcaacaaaa tcctgatcgc
     2521 caaccgcggc gagatcgcgt gccggatcat ctggacgtgt aaagagatgg gcatccgcac
     2581 cgtcgccgtc cactcgacgg ccgaccgcga ctcgctccac gtccgcttcg ccgacgaggc
     2641 gatctgcatc ggcggcccga cctccgcgca gagttacctc aacatctcgg ccgtcatcgc
     2701 cgccgccgaa atcaccaacg ttgacgccat ccaccccggc tacggcttcc ttgccgagtc
     2761 ggcctacttc gccgaaatct gcgaggcgtg caacatcaag tttatcggcc cgcgccccga
     2821 cgtgatccgg cagatgggcg acaaggtgga ggcccggcgc acgatgacgg aggcgggcct
     2881 gcccgtcctg ccgggaagcc ccgagccgtt gcgcgacgag ggagaggcgc ttgcgattgc
     2941 gcgcgagatc ggctaccccg tcatcatcaa ggcgtcggcc ggcggcggcg ggcgcggcat
     3001 gcgtatcgtc cgcgccgaag gtgagttcct gcccgccatg cggactgcct cgaccgaggc
     3061 ggcggccgcc ttcaagaacg gcgacgttta catcgagaag ttcatcgagc gcccccgtca
     3121 cgtcgagatt caggtgctcg ccgacgagca cggcaactgc attcacctcg gcgagcgaga
     3181 ttgctccatc cagcgccgac accagaagct catcgaggaa tcgccctcgc cggtgctcac
     3241 gccggagatg cgcgagcaga tgggccacgc ggcggtcgag gcgtgccgcc gggtcggcta
     3301 ttcgagtgcg ggcaccatcg agtttctgct cgacgaggat cagaagttct acttcatgga
     3361 gatgaacacg cgcatacagg tcgagcaccc cgtgaccgag atggtcacgc tcgtcgatgt
     3421 cgtgcgtaac cagattcgca tcgccgaggg cgaggagatt tcctaccggc aggaggagat
     3481 cgaaatccgc ggccactcca tcgagtgccg catcaacgcc gaagaccccg cgaccttcgc
     3541 gccctcgccc ggtctcatca cgacctacaa cattcccggc ggccccggcg tgcgcgtgga
     3601 cacggccgtc tacgccggct actacgtccc gcccttctac gactcgctca tcgccaagct
     3661 gatcgtccac gcgcgaaccc gcgagctggc catcgcccgc atgcgtcggg cactggaagt
     3721 gatggtgatt gaaggggtta agacgaccat cccgctccat ctggcgatta tggacgaccc
     3781 tcatttccgt tccggcgagt actcgacgaa gttcatggag cagttcttcg agcggcgaaa
     3841 cgcacagggc gcggcccagg ctggctgacg tgaatcgaag gcccgccggc tcgtttgact
     3901 gggcgcgata acgaccttat aattacggac ttttctatgg caggtcggcc ctgtctgaag
     3961 cggttctgaa ggagaagtag agatggctta cgcaatcatc aggtcgggcg gcaaacagtt
     4021 ccgcgtcgaa gagggcgcca cggtgcgcgt cccgtctatc gacaagcagg ccggcgagca
     4081 ggtcgaactc gaagccctcg tcgtcagcgg cgacggcggc atcaaggtcg gctcgcccgc
     4141 cattgacggc gcgggtgtga cgggcacggt cgtcgatcac ggccgcggca cgaagatcat
     4201 cgtcttcaag aaaaagcgcc gcaagcagta caagcgccgg cagggccacc gtcaggacta
     4261 cacgacgttg aagatcgact caatcgggta aagacagagg ttagaggtca gaggttagag
     4321 gtcagtagaa ggcaagctgt tttcactgac ctctgacctc taagctccga cctcttacgg
     4381 ggtttagtta tggcacataa aaaaggcgtc ggctcatcgc gcaacgggcg cgattcacac
     4441 tcgcagcggc tcggcctgaa gaagttcggc ggcgagcagg tattgggcgg caacatcctc
     4501 gcgcgccagc gagggacgaa gtggaagccg ggcaagaacg tcggccgcgg taaggatgac
     4561 acgctgtttg cgctcgtcga tggtgttgtg aagttcgagg acaagggccg caagggcaag
     4621 ttcatcagcg tctacccggc cgtggcggcg gcgttgtcgc ccgcgccaga ggcgcccccg
     4681 gcggttgcag catgatttgc gaatgagcca ctctcgcgct ctgatttctg acgccccgcc
     4741 gagtgccacc actcgaagcg agggcgtcag tttcgttagt agctgacagc tttgttcatc
     4801 gaccgcgtca aaattcgagt ccgagggggt cacggcggca acggcgtgac ggccttccgg
     4861 cgtgagaagt tcgtgccacg tggcgggccg tcgggcggcg acggcgggcg cggcggcgac
     4921 gtgtggctcg aagccgccac ctcgctcaac acgctgctgc acctgcgtta caaccccgaa
     4981 cactcggccg agcgcggccg acacggcgag gggtcgaacc gctcggggcc ggagggcggc
     5041 gacatcctcg tgcgggtgcc ggtcggcacg caggtcttcg acccagagac gggcgacatc
     5101 ttttgcgacc tcgccgttga ggggcagcgt tggctcgccg cgaagggcgg gcggggcggc
     5161 ttcggcaact cgcacttcgc gacctcgacg aaccgcgcgc cgcgctacca ccagcagggc
     5221 agccccggcg aggagcgcga gctacaactg gagctaaagc tgctggccga cgtgggactg
     5281 gtcggcttcc cgaacgcggg caagtccacg ctcatctcga ccgtctcggc cgccaagccg
     5341 aagatcgccg actacccgtt cacgacgctg gagccgcacc tcggcgtcgt cgatctcggc
     5401 gagtttcgca cgttcgtcat cgcggacatc cccggtctca tcgaaggcgc gcatcagggg
     5461 gcgggcctcg gcgaccgctt cctgcgacac gtcgagcgga cgaaactgct gctgcacctc
     5521 gtggacgtat cctccgcctc gggccgcgac cccgtcgagg actacgagac gatcaaccgt
     5581 gagcttgccg catatgacgc ggcgatggcc gagcgcccgc agatcgtcgt cgcgacgaag
     5641 ctcgacgcgc tcgacgagcc ggagcgtctg gagcatctgc gcgaacgcgc ggcgcgcgac
     5701 ggacgggagt ttcacgccgt ctcctcggtc gccaacacgg gcgtgcgtga gcttgtgtac
     5761 gccgtcgggc ggcggctcga cgagatcaaa gagtccgaga gcgtcatcga gctggatgcg
     5821 gagtggtcag gcgagggcga ggcgtccacg ttcgggcgcg cggattgaag tgatgacagg
     5881 ggcacggcgc atcgcgctct acggcgggag cttcgacccg gttcatctgg ggcacctgac
     5941 ggtggcgcgc agtctgaccg agttgttcgc gctcgacgaa gttcttttca tcccggccca
     6001 cgtcgcgccg cacaagcgcg gtcggcgcgt cgcaccggcg ctcgaccgcc acgccatgct
     6061 cgcgctggcc acgcaggacg agccgcgctt ccgcatctcg accgtcgagc tggacgcccc
     6121 cgagcggccc tacacggtcg agacgctggc gcacttctgc gaggcggcga gtcgcgacgc
     6181 ttcgcgtctc ttcttcgtga tgggcgccga ctcgtgggag gagatcacga cgtggaggga
     6241 gtgggagcga gtgctcggca tggcctcgac catcgttgtg acgcggccgg gccacgagct
     6301 tgaagtgggc cacgtgacgc cgtcggtgcg agagcggatc gtggacttgc gcggcgctga
     6361 tgtgaaacag gcggcggaag aagttgacgc agatggggtg aagatttttt tcaccgacgc
     6421 ggccgacgtt gacatctcgt ccacggcgat tcggcgcggc gcagccggcc gcggcggggc
     6481 gctggtgttg gaaaactggg tcgcgcccga ggtggccgag tacattcgga aatacggtct
     6541 ttacagggaa gtacatggaa cagaatcgga cgacagcgcc ggaacggaaa cggcgcacga
     6601 acaagaaggc tgaagaggcc gtcgagacgc aggaggcgac cgcggccgac gcgacgccgc
     6661 tcgcccccga ggcggccgag gagggactcg acgcccggat gctcgccgcg ctccacgccg
     6721 cgggcgacaa gaaggcgcac gacctccttg ctctcgatct gcgccccgtg gcgagcttca
     6781 cggacttctt cctgatcggg agcgcgcaga acacgcggca ggtgcaggcc atcgccgacg
     6841 agatcgtcga gcgactgcgg gcgatggggt cgagggccgc gcgcgtcgag ggctacacga
     6901 gcgccgagtg ggtgctcctc gacttcggcg acttcgtctt tcacatcttc gagcagaagg
     6961 cgcggcgctt ctacgaactc gaacgactct ggcgcgacgc ggcgcgcgtc gcgttgccgc
     7021 cggagttgtc ggccgctgct gctgctgacg acgcgcgcgc gggcagcgac gtggaaggct
     7081 ctttgagaag cgaacagtga tcgagatcgt ggcgcgttcc ggggcgatgc gcgaggcgct
     7141 gcggctcgca cgccgcgtgg ccccgaccaa cgccaacgtc ttagtcacgg gcgagtcggg
     7201 cgcgggcaag gacgccctcg cacagttcgt ccatgagcac tcgacgcgcg cggcgcgcga
     7261 cctcgtcaag atcgactgtg cgtcgctccc ggccgggttg ctggaggcgg agctgttcgg
     7321 ctacgagcgc ggcgccttca ccggcgccat cgcggccaag cccggcaggc tcgaagcggc
     7381 cgacggcggc acgctcgtct tagacgaagt cgcgcatctc tcgacggacg cgcaggcgaa
     7441 gcttttgcgg gtcatcgagc ggcgcgagtt cgagcggctc ggcgggcggc ggacggtgcg
     7501 tgtggacgcg cggctcgtgg cgctgacgaa cgtcgatctg gacgaagcgg tcagacgccg
     7561 cgccttccgc gaagacctct tctaccggct gaacgtcgtc cgcatcgacc tgccgccgct
     7621 gcgcgagcga cgcgaagacg tgccggcgct ggcccggcag ttccttgaaa agtatgccgc
     7681 caagcacggg cggcgactga agagtttgac ggccgaggct aagagcttgc tcgccggcta
     7741 cgagttcccc ggcaacgtgc gcgagctggc gaacgtcgtc gagcgcgccg tcatcgtcgc
     7801 cgagggcggg cgcgtcgagg cgtctgacct gcccgagtct gtcagggcgg ccgtccgggt
     7861 ttcgtcaaga cggacgatgc gcccgacgct cgccgaggtg gaggacgagt acatccatga
     7921 gacgctcgcc gccgcgggcg gcaacaagac ggaggccgcg cgcgtcctcg gcatcagccg
     7981 caagaacctt tacgagaggc tggcgcgcag cggcaacaaa gaggtcggag gtcagaggtc
     8041 agaggtcagt aaaagaaatg ctgatgcgga tgagacaacc actgacctct aacctctagc
     8101 ctctaacctc agctttatgc gtttgcgcct catctgggtc ggcaagacgc gcgacgagca
     8161 cctgcgggcg ctcgtggggg agtatttgaa gaggctgggg cggtacgcga agtgtgaggt
     8221 ggcggaggtg cgggagggcg ggagcgccga cgagcgcacg tgcctcgaag aggagggcaa
     8281 acgaattacg gcctcgctcg catctgatgc cgtgacggtt ttactcgacg tgggcgggcg
     8341 cgaatatagc tcgcacgagt tggcggccca ggtcgaggtg tggcaggggc gctccgtcaa
     8401 ggaggtggcc ttcgtcgtgg gcgggcacct tggagtttcg gatgacgtga ggcggcgggc
     8461 cgacattcgc tggtcgctct cgcgcctgac gctgacgcac gagatggcgc gggtcgtcct
     8521 cgtcgaacaa ctctaccgcg cgtacacgat cctgcgcggc gtgccgtatc agaagtgaag
     8581 gagcgattag ccaatagctt ttagctatca gctaaaagtt gtccgttgtt gagggcgatt
     8641 cgccgtggac gccggaagat tcaagtttag gggagcaaac tactagccac ggactactga
     8701 caaaggacag cacttagcta acagctaatc gctaatagct ttctgccttt caaggggttt
     8761 aagaaacgga gaatagatgg ataagaaaaa aatgaagacc taccgcgacc gcctgctgga
     8821 gcgccgcgag tcgctggtga gccaggtgac ggaggccgag ttgagcagcc gcgagcgcga
     8881 catggaggcg acgcaggacc cggccgacat ggccgccaac gcctacacga aggagttgct
     8941 gatctcgatg tcggccaacg accgggcgct gctggggttg atcgacgagg cgctggcgcg
     9001 ggtcgagtcg ggcgactacg gtgagtgcgt caactgcggc gagctgattc tggagaagcg
     9061 tctcaacgcc gtgccgtggg cgcgctactg cctgcgctgc caggacatga aggagcaggg
     9121 gctgctcaac gaagaggaag agtagtctct tcggcggccg attctttgtg aaggccccgc
     9181 gcgccggaag cttccggcgc gcggggcttt tcgtttatgc tctgccaaac gcgccggagt
     9241 tatcctccga cacgcgcgcc gtgccgtgcc gcccgcgccg ggccgcgagc acaaacgacc
     9301 tcaccatgcg tatccccgca aggctcgacc gactctgcga cgccgcgctg gcgctcttct
     9361 acccgctcgc gtgcgcggcc tgcggcgcgg agggcgtcga ggcccgcgcg gactttcccg
     9421 tctgcgccgc gtgctgggcg cggacccgtg tcctcactgg cgatgagacg gtctgctgga
     9481 agtgcggcac gagagcgcga gccgaagtgg acgcgtcgag tagggagacg gtgcgctgtc
     9541 gccgctgcga ctcgtgggcg ttcacggcgg cgcgcgcgtg cggcgtctac gaaggcgcgc
     9601 tgcgggccgc cgtgctcacc ctgaagcacg agccgcgcgt gggcggaagg ctcgcacgac
     9661 tgatgtggga ggcgcagcgc cggccgccgc tcgattgctc gactcgcgtc gtgcccgtgc
     9721 cgctccaccc ggagcgggag cgcgagaggg gcttcaatca ggcagcggtg ctggcgcgcg
     9781 ccgtcgcccg acgcgccggg ttgccctgcg acgagcagag tctcgtccgc atcacacaca
     9841 ccgagcagca ccgcgccggc atggacgcac gcgcccggcg cgagacggtc gagaaggctt
     9901 acagggtcgc gcgcccacgc ctcgtccgag atgaaaagat tctgcttatc gacgacgtac
     9961 tgaccaccgg cgccaccgtc tcctcctgcg ccgccgcgct ccgggccgca ggcgcgcaag
    10021 aggtatacgt gctcacggtc gcgcgcgcgg gcgcatagat tcggatttca actgcaagta
    10081 gatagaaagc ccggccggct cctgagcagg agccggccgg gcttcgttgt tgagggtcgt
    10141 agctgtgtcc tgacccggaa ggttaggcca gggcctcgac ggcgctcgcc ggattcactt
    10201 cgatgtccac cctcatgtcg gtgatcacct cggggcagac ggcgcaggcc gccgcgccag
    10261 actgctcgaa ccagcgacac tgcgggcgga tcgaacactc gggcaactca cccggagcgg
    10321 cgcggggcgc ggccgcaagc tcctcgtcga tgacgccgca acgctctccc gtccattgcg
    10381 agcaaccgcc cttggcgcac ggactgctga aacggaaacg cttctccggc gggcgtccct
    10441 cgtgtgcgat ttgcacgaac tcccggtcga tgacgatccg atcactggcg aaagccactc
    10501 ggccgtccga catgacgatg ccgaccagga cggccccttc ctcacaacgc gcgctcgggc
    10561 acagaatggt tttatcatcc atactgcctg ccttgtctcg gtgcttacat gcggacttca
    10621 cgcaccccga ggttcgggat gcccttgttg aacaggtctt tggcgaacct ggccatgatc
    10681 atcccgcgcc actccgggtg gatcatggcc gcgaaccccg agccaccgcc cgcgccggcc
    10741 tttccggccc cggccttttt cccccccgtg tcaatcttcg ccagctcctg catgacggcc
    10801 gcgcgaatcg cggcggcaat tctatcttcc gcgtccgtgg acagatcaag gccgtcgagt
    10861 ttgacgatga actctccttt agccatgaga ttttctcctt attggaaatg aagttaatgc
    10921 ggagaacgcg aggagttgtt ctccacagag ttaagagccg ttgaggcgca aggggcaaga
    10981 ccaatgaggc gcgcaatgta ccagatgagt gggctaaata caatgacaat ctgagacgag
    11041 gacgccggat gccaagagcc gctcgcgact ccggcgcggg gccggacgcg gcgcgggtac
    11101 acatcgcaca gtggatcgca tcatccactg tgcgatgtct ggcaccggcg cggcgacctc
    11161 tgtatgatgt ccgcatgaga accttctatc cgcccgtcga gccatacgat gcgggaatgc
    11221 tcgcggtctc gcccgtgcac acgctctact acgagcagtc gggcaacccc gacggcctcc
    11281 ccgtggtctt cctgcacggc ggccccggcg gcggcaccgt ccccgtctac cgccagttct
    11341 tcgaccccga ggcttaccgc atcgtcctct tcgaccagcg cggctcgggc cgcagcacgc
    11401 cgcacgccaa cctcgaagag aacaccacct ggcacctcgt cgccgacatc gaacgactgc
    11461 gcgaacacct cgggattgaa aactgggtcg tcttcggcgg ctcctggggc agcacgctcg
    11521 ccctcgccta cgcgcagacg caccccgcgc gcgcccgcgc gctcgtcctg cgcggcatct
    11581 tcctctgtcg ccgcaaggag attcgctggt tctatcagga gggcgcgagc gccatcttcc
    11641 ccgacgtgtg ggaggagtat gtgcgtgtca taccggaacc tgagcgcgac gactttcttt
    11701 cggcctatca ccgtcggctg acgagcgacg acgaggccgt gcgcctcgaa gccgcgcgcg
    11761 catggagcgt ctgggagggt tcgacctcga aactgttccc tgactcgtcg ctcatcgagc
    11821 acttcggcga gccgcacatg gcgctcgcgc tcgcccgcat cgagtgtcac tacttcgtca
    11881 acgacgcctt cttcgagacc gagaaccaac tcgtcgagaa cgtcgggaag attcggcaca
    11941 tcccggccgt catcgttcag ggccgctacg acgtggtctg ccccatgatg agcgcatggg
    12001 aactgcaccg cgcctggccc gaagctgatc tccgcgtcat cgccgacgcc ggccactccg
    12061 ccaccgagcc ggggatagtc agcgcgctcg tcgaggccac agaccggttc aaaaacggtg
    12121 ataagtgatg agtgataagt gataagagag aagcggttgt cgtctttgtc tcatcactca
    12181 tcactcatca ctcatcactt tccttttgcc ttcttcagac tgctcccgta aaatctcggg
    12241 gccatatgaa cggacgtgtt ttagtcctga acgcatccta cgagccgatc aacgtttgca
    12301 ccgagcggcg cgcggtggtg atgattttca agggcgtcgc ccgcatggag gagcacaacg
    12361 ggcacatgct ccattcggcg cggatcacga tgcacgcgcc ctcggtcatt cgcctgaccg
    12421 agtacatcca catccccttc aagaaccgca cgctctcgcg caagaacatc ctgctccgcg
    12481 accactcgac gtgccagtac tgcggccgcc aactgccgcc ctcggagttg acgctcgacc
    12541 acatccaccc gcgctcgcgc ggcggcgagt cctcctggga caacctcgtc gcctgctgca
    12601 aggcgtgcaa ccatcgcaag gggagccgga cgcccgagga agcgcggatg cacctcatcc
    12661 gccggccgcg cggcttctcg ctccacgtca cgcgccagat catgcgcttc atggggcgtg
    12721 ccgaccgcac ctggcgcaag tacctgttct acgagtcgga caacggcgag gtgtaggttt
    12781 cgagagccgg agcacgcgcc gcaagtgaat cccctgggag gacaatgatg acgagcttat
    12841 tgtttggcat gccgcagggc gccgagtggc tgattatcct cttcgtcgtg ctgctcatct
    12901 tcggcagcac gcgcctgccg cagctcgccc gcggcatggg caagagcatc aaggaattca
    12961 agaagggcat caacgaaagc ggcgacgacg acgaggtcga agagacgcgc cgcgcgcgcg
    13021 agctgcgcgc cggcgagacg gcccgcacac gcgaagacga actcgccccc gacaagctat
    13081 ccgccaacaa gccgcgctaa agaaggcaaa agtaaaaagg caaaaggcaa aagtgggagg
    13141 gcgtgactgc catctcactt ttgccttttg cctttttact tttgccttac tcggccctgc
    13201 ggccgaggat gagtgtggtt ttgatcgaga gcgtgtagtc catgtcgttg cgctcctcgt
    13261 cgatgatgcg ctcgacctcg ccgagcacgc gcgtgcgggc gtcatcttcg ggcagcgatt
    13321 cgagccagcg gccgagcaga aagtcgcgca ccagcggcgc gttcaggaac tccgcgcccg
    13381 tcgggtagtt gaactcctcg acgttcgtcc acgagcgcac gtcgtcaagc ccttcgcgcg
    13441 cggccaaccc ttccaggtcg gagacggtcg gcaactcgtc gatgagcgcc ccgacgcccg
    13501 cggcctgctc gtcgaagccg gcgctggcta acgcctccca gtagacagag aagaactcgc
    13561 cgaagctcga cgcgctcgcc acgtcgagcg cgaccgcccc gccgggcgcg gcgacgcggg
    13621 tcatctccgc cagcatctcg ggcagccgct cgggcgccag catcgacgcg tcgccgacga
    13681 ccagctcgaa cgtctcgtcg ggcagcgaga gggcgtccaa ctgctcgtga ctgaagacgg
    13741 tgtcggaagc cgcaggcgtg gccgccgcct tcgcccgcgc cagcgccagc cgctcctcgc
    13801 tctcatccac gcagaccaac tccacctcgc ggcccgcgcg cgccttgagc gccagcgcgt
    13861 gctcgcccgt gcccgcgccg acgtagagca cgcggccctt cttcggcaac tggacgtgct
    13921 cgtcgatcag ccccgcgaac ctctcgcccc agtccgtcgc cacgtacaaa tcgtggagaa
    13981 aggccagctc cttttcggtc ttgccgctag tcataaaaaa gggtgatgag tgatgagtga
    14041 taagtgatga gaaagaccgt caatcgtgaa tcgtaaatcg tgaatcgtga aagacggttt
    14101 ctccgattca agatttacga tttaccattc acggctttat cacttatcac ttatcactta
    14161 tcactcatca cttatcactt ctccatcgtc ctcccagtaa acttttcctt gcaggcgctc
    14221 gggcaggcag gacatctcct cacgcgccgc cgcgtcgtcg tggacgtagc ggtagccctg
    14281 tccgtagccg acgcgcttca tcagcggggt gacggcattg cggaggtgga gcggcaccgg
    14341 ctcgcgcgcg gtctcggcgg cgtcttcgag cgcgcggtgg taggcgcgtt tgacggcgtt
    14401 cgacttcggc gcgcgggcca gatagacggt cgcctgcgaa agcggataga gggcttcggg
    14461 cagcccgatc aactccgtga tcttcgcggc ggaggcggcc tgcaccatcg cctgcgggtc
    14521 ggcgaggccc acgtcctccg aggccagaat gcagaggcgg cgggcgataa aaatcgggtc
    14581 ggcgccgccc tcgatgagac gcgccagcca gtagagcgcg gcgttcacgt ccgagttgcg
    14641 taaagatttg atgagggcgg aggcgaggtt gtagtgctcc tcgccgccct tgtcgtagcc
    14701 gtagcgggcg cggccgaggg attcggcgac ggtctcctcg gcgacgttgc gcgcgccctt
    14761 cttgtcgggc ggagtggact cgacggccgc ctccaacgcc gagagcgcga cgcgcgcgtc
    14821 gccgcccgcg gcgctcgcga gacgcgccag caactcgtcg gcgacgaccg ctttgagacg
    14881 gccgaggccg cgctcctcgt cggcgagcgc gcggcgcagg agcgtttcca cgtccaaccc
    14941 ttcgagcggc tggagcgtga ggacgcggct gcgcgagagc agcgcgccga tgacctcgaa
    15001 ggaggggttc tcggtggtcg ccccgatgag cgtcaccgtc ccgtcctcga ccgcgggcag
    15061 gagcgcatcc tgctgcgcct tgttgaagcg gtgaatctca tcgacgaaga gaaccgtgcg
    15121 ccggcccgtg cggcggcttg cgcgcgcctc ggcgatggcg gcgcgcagat cacgcacgcc
    15181 cgagaagacg gccgagagtt ggatgaagtg ggcgtccgtg tggcgtgcga ggatgcgggc
    15241 gagggtggtc ttgcccgtgc cggggccgcc ccagaagatg agcgagggga gcttgccgcc
    15301 cgcttcgagc aggcggcgca acacgcgccc ctcgccgatc agttgttgct gcccgacaaa
    15361 ctcgtcgagc gtccggggcc gcatgcgttc ggcgagcggg gcgagcgcgt cggacggggc
    15421 cgtgtcctcc tccggttcgg cgaacagact ttcgttcgtg cttcgcggca taattggaac
    15481 tctttcggga caaggatacc acggccccgc aggcgcgcgc cgcccgcgcg gagtggcgcc
    15541 gctcgagtgt gccgacgtaa tgctcggccg cgcctgacac atatttgtga attgaagggc
    15601 aggcgaaagt gagtacgaat aactgaatgt cagggtaaaa tcttggtgga acgcggccgc
    15661 gccctcctgc gtatatggag acgtgccaga cgcttccggg gccgcgaatt aattccggcg
    15721 acggcggcta agctagagtg aggtgataat catgaaagac atgaaagata tgttgcgtat
    15781 aatcggaatc atccttctcg gagttttcgt tctcgccgtc ggagtaccgc tcgtgttcgc
    15841 tgccgccggc atcgttctcg gcggcatcat tcacatcacg gtctcgttga tcaagatcgc
    15901 cgtggtgctc gccgtcgcct acctgatcct cgtcggcgtc cgcgccgtat tgaggtaatc
    15961 cccgacgccg ggcgccgcca acgtcttgcg agactggcgg cgcccggcgt ttcccccccc
    16021 cgtgtcacag cgcgcaactg ccgcccgcat cctccgcgcc cttcccaaca cacctcgaac
    16081 tcggacgacc tccgaacgtg atcgtctggc gccctatgcc ccgcggacaa aagacgcggg
    16141 ccgccgtgtc agtttgaaaa atattctcac tcaacactct cctgccggcg gtcacgcgaa
    16201 atgtttcgtc tcaaaattac attaacggta agagggcggt gggccggcgt aactgacccc
    16261 gaatgacgac cggctggaag gtcgaactcg gaggcgaaat cggctcactg ctgaggcgtg
    16321 acaagccctg ggagattggg tcatactgcg cccgcgctga caggccatga atctcctgcc
    16381 tctggctact cggggcgcca cggttcctct cgcacggccg cgcggcgctg gcaatttcca
    16441 ccatcaccga tgccttgggt gtgactcctc tctgcgtcat gtcatccttt ccagggaaca
    16501 cgaaccgcgt agagccgccc gcgcggcgcc ggtgtcactc ctctagttta tgcgtccgtg
    16561 ccggcggcgg gacgaaatct caacccacag gaggaatcag cagatggcga ccaggaagaa
    16621 gagcggtgcg tcgaagaaga gtgcgggcgc atcgcgaaag agcagcactg taaagagagc
    16681 gggggcggct gtgaagtcgg gcgcgtcaaa gaagaccggg gcgtcgaaga gggccggggc
    16741 cgcgaggaga gccggcgcga cgagagctgg cgccgccatg cccgtggcgg cggcggcggg
    16801 cgatgccgtg ccattcctgc gacgccgcga tgaaggcccg gaggtagaca atctccagga
    16861 ggagctgtgc gacatcggct acctgacacg cgcgcagaag gcgctggggc cgggcaagtt
    16921 cggcccgcgg acggaggagg cgctcaagca cttccagcgc gacaacttca tcgccgaaac
    16981 gggcgtctac gacgaggaga cgcaggcggc catccgccag ttgaatgagg gcgtcaagcg
    17041 cggcatcgag ggcaacatcg tgcgcggact tcagaaccgc ctcgtctcgc tcgggttcat
    17101 gacgcaggtg atagcggggc aggggaagtt cggcccgcag accgaggccg cgctcctcgg
    17161 cttccagatg gctcagggca tcatgcagac cggggtgctg accgacgaga cttatcgcgg
    17221 cctgctcgcg gcggcgccgc acccgacgcc catcaccgat cacggcgatc caacgcgcat
    17281 cgacaccgtc ctcccctcgg aaggccgcgg cttcacgacc taccggcgct cgcccggcgg
    17341 agccgaccag ttcggccgcg cctccaccat ccgcgccatt acggatgtcg ccgagacgtg
    17401 gagcacgcgg cacgcggcgc cgcgcctcca gatcggcgac atcagccgac gcggcggcgg
    17461 ccgcctcgaa ccccacgata gccatcagag gggcgtggac gtggacatgc gccccatcag
    17521 gaacgacggc cgcgagcagc cggtcaggtt cgatcaggcc ggctactcgc acgagctgac
    17581 aaaggagctg tgccagctca tccgcgcgaa agtccccggc gtccgcatct tcttcaacga
    17641 cccgcggctc atcggcctcg gcctcacggc gcgccttata ttccacgacg accacctgca
    17701 cgtccgcttc cccagctgag gaagccgaaa cgcagtcaac aaagatgaag gggcgacgga
    17761 agacccagtc ttccgtcgcc ccttcagttg tcgtgaataa ctcgcgcgcg gtcgcgccgc
    17821 cgcccaacgt gcgttgaagc gaagcgcgac tttatcaccg gcgggggaga ggtcagcgca
    17881 gttcgcgcgc gagccgtctt ctggccttct ccatctcctt cttctcctcg ccattgacgg
    17941 ccggatagcg gggcttgagc gagcggagct ttcgcaccat caggtcggcg acggcgagcg
    18001 cggaaaacca tcgatggtct gccgggacga cgtgccaggg cgcggccctg gtgctggtgc
    18061 gcgcgagcat ctcttcgtgg gccacgaggt gctcgtccca cagttcgcgg ttctccaaat
    18121 ccgcggcgga gaacttccac ttcttggcgg gttcctctgt acgctccagc agccgctcgg
    18181 cctgcttctc cttcgagagg tgcaggaaga acttcaggac gaccaccccg ttatccgtca
    18241 ggtactgctc gaagttgttg atctcgccca tcctccgctt ccagatgtct ttggacttgc
    18301 tggatgtggg cagattctga tcgcccagcc cctcggggcg gacgcgcggc gcgacgacct
    18361 cttcatagta ggagcggttg aagaaggtga tccgcccgcg ctccggcacc tcccggtgga
    18421 agcgccacag gtactcgtgc ttgttctcgg catcggacgg cttcttgaag ttgacggccg
    18481 tgcagccctg cgggtcgagg ctcgacgcga cgtgcttgat ggtgccgtcc ttgccggagc
    18541 cgtccattgc ctggaggatg accaagagcg cgtgcgtctc ctcggccatc agcttgtcct
    18601 gtagggtcgc cagctcgccg cagtcggcct cgattttctt accggccgcg tcttcgtccg
    18661 cgaagccccc ggtgaaagcc gtgtcgtagt tcttcaggcg cgggcggctg cccggcctga
    18721 ccatgaaatc attttgtctg cccatgctcg ttacggaaac ctttctgaat gccggcgaca
    18781 cggggttaag acgccaacat ctttcgcgaa aaccaagttg attgatgaag tcatcggagg
    18841 ggataaagcg aatggggtcg gagtcgtcgt catacgttga agcggaagtc tacgacatcg
    18901 ccttcccgca tgacgtactc cttgccctcc agccgcgtca gacccttctc gcgcgccgcg
    18961 gcgcgggagc cggcgctgag aaggtcttcg tagccgacga cctcggcgcg gatgaagccg
    19021 cgctcgatgt cggagtggat ggcgccggcg gcctgtgggg cgcgggtgcc ctgcgggata
    19081 gtccaggcgc gcacttccat ctcgcccgcc gtgagaaacg acatcaggcc gagcaggtgg
    19141 taggcgctct tgataagact gtcaacaccg ctcgactcga ccccgagcga ctgtagatat
    19201 tcgcgccgct cttccggcga gagtgcgacc agctcggctt cgagctgcgc gcagatgacg
    19261 accgcctcgg cgccctcacg ggcggcgacc tcgcggacgg cgcgcacgtg tgcgctcgcg
    19321 tcggggtctg agagcgtcgc ttcgtccacg ttcgcggcgt agatgttcgg cttcagggtg
    19381 agcaggaaga agttgcgcgc gagtgccaac tcgtcatcgc tcagcgcgac gctgcgcgcc
    19441 gggcgaaact cttcgagggc gggctggagc ttgtcgagca cgccgagttc ggcgactgcc
    19501 tgtttgtcac cgctcttcgc cgtcctctgc gtgcgctcgc ggcggcgctc gacgctcgtg
    19561 aggtcggcca gcgccagctc gatctggacg gtctcgatgt cgcggcgggg attgaccgag
    19621 ccttcgacgt ggacgacctc ttcggactcg aagcagcgca cgacctgcac gaccgtgtcc
    19681 gactcgcgga tgttggcgag gaactggttg ccaagccctt cgcctttcga cgacccgcgc
    19741 acgaggccgg ggatgtccac gaactcgatg acggcgggct tgatgacgct cgtcttgacg
    19801 aggcgggcca gcggctccaa acgctcgtcg gggacgggga cgatgccgac gttcgacttg
    19861 ttactggcga acggatagct ggccgcgagc gccgactgct gggacgtgag cgcgttgaag
    19921 agcgtcgtct taccgacgta ggcgagaccg gcgatgcctg cgcgaagcat aggaaacctc
    19981 tgtctgggat gaccgctgaa ttgtgttggc taacggcaga catctaagca tccggcgtca
    20041 ggggttgtcc agccgccccg gcgcacgctt tcggacgcgt gagggtgttc gcgtataatc
    20101 gccgggttct tcgagagcca gtcgcggccg gcgtgcagcg cgcggcgtgt cagcgcgccg
    20161 ccctagtcac cgccgccgcc cttcaactcg gaattatgcc cttcgcaatc ctgctggcgc
    20221 tcgccgccgt ctcgggcggc acgctgctca cgtacctcta cgaccgcgag gcgtcgccgc
    20281 tcgtgcgcgt gtgcgagggt ctgtgtaccg gcttcgccgc gctcggcctc gtcggtttca
    20341 tcatcgcctc cttcgcgggg ctacagcctg ccgcgctcgt cgccgcgggt ctcttcgtcg
    20401 cctccccgct catcctttta cgcaaggccg aatggcgcgc tcgcgtgcgc gttgacgcgc
    20461 gtgaggcttc gcgcacgttg gggcgggcga ttactttccg tagcggcggg gcgacgtttg
    20521 tgttctacgc ggcggcggcc gtgttgtgct ggctcgtctt ccggcaggcg atgtacgcgg
    20581 gcgcggacgg catatttacc ggactcgata acaacatcgg cgatctgccg ttccacgtcg
    20641 gcatcatctc gggcttcgtc cacggcgaca acttcccgcc cgagcatccc gagttcgcgg
    20701 gcacgcgtct gacctatccg tttctggcag acttcctgac ggcgatgttc gtgcgcgccg
    20761 gggcctcgct cgaaggcgcg atgttctggc agaacttcac gctcgcgctc tcgttcgtcg
    20821 cgctgctcgg gcgctgggcc gcgctcgtca cgggggagcg cgccgccggc ttcctcgcgc
    20881 ctgcgctcgt cctcttcaac gggggcttgg gcttcctgca agtgttcggc gaggctgcgc
    20941 gcggcggcgg cctactgcac acgctcggca atccaaccgt ctactacacg atgatgggcg
    21001 acacgtacaa gtggggcaac ttcgtcacga tgctgctcgt cccgcagcgc gggctgctgc
    21061 tcggcttccc gctggcgatc atcgtgttga cgctgtggtg gcaggcgact tcgggggaaa
    21121 agggggaagg ggtaaagggt aaaggggaag agggaagagg gaagagggag cacagggaag
    21181 ccgcaacgcg caagggcgag gcgacgaaga aggggaagcg caacgtgagc agttccgacg
    21241 cgcgtgcgaa gtcgtcggcc gcggcggcgc agcttacaca ctccacacct ttccccctta
    21301 ccctttcccc tttccccttt cgccaaatgc tcgcggcggg cgtcattgcg gggctgatgc
    21361 cgctggtgca cgcgcacagt ttcgtcgtgt tgatggggat gggcgggtgt ctggcgttgc
    21421 tgttgagctg gcgcggggcg cgagacgcgg tggcgcgcgg cgaggtcggg aaggaagcgg
    21481 gcgcggggtg gcgcgggtgg ttcgtgttct tcgcggcggc gacggtcgtc gcggcgccgc
    21541 agatgttgtg ggcgacttac agaagcgacg tgcaggccgg gagcttcttc gggtggcagc
    21601 tggggtggga tcgcggcacg ctgaatccaa tcgtgttctg gttaaagaac acggggctgt
    21661 tcatcccgct gctcgtcgcg gcgctcgcgt ggcgggggca gaagcctttg gtctcgcgcc
    21721 ggctgctcgt cttctacctg ccgtttacgc tctgcttcgt cgtcccgaac ctgttgaaga
    21781 tgtcgccgtg gatttgggac aacatcaaga ttctcttcta ctggtgggtg gcctcggcgc
    21841 cgattgtcgc gctgctcatc gtgcgcctgt ggcgtcgggg tggcgcgtgg cgcgccgccg
    21901 ccgccgtgtc ggttgtggtg ctcacgcttg cgggcgcgct cgacgtgtgg cgcgtggcct
    21961 cgggcgggat gcgccagccc gtgttcacgc gcgagggagt ggagttcgcc gagatgttga
    22021 aacgcgagac gccgccccgc tcactcgttc tccatgcgcc gacgtataat cacccggtct
    22081 acctgacagg ccggcgctcg ctgatgggct acgccgggca cctcggctca cacggcatcg
    22141 actacacgga acgcgagcgc gacttcgcgc gcatcttctc gggggggccg gaggcggagg
    22201 cgctgttgaa gcaatacggc gtcgattacg tcgtcgtcgg gccgctcgaa cgccggggcg
    22261 gcgagatggc ccgcatcagg gccgtggtca acgaaacgtt cttccaacgc tacacgaagg
    22321 tcgggcaaac aggtggctac agcctctaca aaacgtcgcc ctaagaaggg cggcgcggga
    22381 gcgaagcggg cggcggccgc ggcggagcgg gcgcaagggt ggccggagcg cgagcgcgtc
    22441 gaagtctccg agcgcgtctg gatggcggcg agtctcgtca tcctcgcggc ggccgtcttc
    22501 ctgcgtctct acgcgctgga gctgaggccg atgcaccacg acgaggcggt caacggcttc
    22561 ttcctgacgg gcctgttccg tgaaggccgc tacagctacg acccgacgaa ctatcacggc
    22621 ccgaccatct actacttcac gctcccaagc gtctacctct tcggcctgaa gacgttcgcc
    22681 gtccgcctcg tccccgtctt cttcggcgtg ggtgtggtgt ggctcgcgct ctgcctgcgc
    22741 cgctgggtgg ggccgctcgg cgcgctcacg gccgccgcgc tcctcgccgt ctcgccgggc
    22801 acggtcttct attcgcgcta cttcatccac gagatgatgt ttctcttctt cgccctcggc
    22861 gcggtggtcg gagtgcttcg cttctacgac acggggcggg gcgagtatct gctgctggcg
    22921 tcggccgcgc tcgccctcca ctttgcgacg aaggagacgg cctttatgac ggccggggtg
    22981 atggtgctgg cgacgctcat cgcgtggggt tacgcgggct acatgcgcgg gcggccgcac
    23041 ccgttcgccg cgcgtgaggt ggcgagtggt cgcgcggtcg agccgggatt gatggatgtc
    23101 gtcgcgcggc tcggcgggtg gccccgcctg tggccgctgc tgctggcgtc gctcgcgctg
    23161 ttcgtcgcca tcaacgtcct tttctactcg tcgttcttca ccaactcgaa gggcgtcggc
    23221 gacgctctgg aggcgctgaa ggtctggtcg aagacgggcg ccagcgagtt ccacgcgaag
    23281 ccgtggcaca cgtatctcaa gtggcttttg caggaggagt cgccgctgct ttggctcggg
    23341 acggtgggcg cggcgctggc cctgtggtcg gggcgcaacc gcttcgccgt cttcgcgggc
    23401 gcgtgggcct tcggcacgct cgccgcttac tcactcgtgc cctacaagac gccgtggctg
    23461 gcgctcaact tcaccgtgcc cctggccatc gccggcggct acgccgtgca ggaactcgcc
    23521 gagaccgcgg cccacgcgcg ctctggcacg cagcgactct tcccggccgc agtcctcagc
    23581 ctttcgctct tcctgttggc ggtcggcctt ggctttgcac atgtaactac tccatctgaa
    23641 tatttgtgca gggccgtcgg cgtgtggtgc accacgccgt tatcggcggc ggacggcccc
    23701 ggtctctcta gcgagtgggg cgcgttggtg gcgctcgtcc tggcggcgct cgtcgtcggc
    23761 tggtgcgtgt ggagactgta cacggcgagg aagggcggcg cggcgtggcg gcgtagtgcg
    23821 ccggcgctgg cgctggcggc gttggccgtc gtggtctgca catatcaggc gtgggtggtc
    23881 agcttccagc agtacgacaa cgaccagtac ccatatgtct acgcgcacac gcggcgccag
    23941 tttatcagcc tcgtcggcga gatcgagcgt ctggtggcgc gcgccggcac aggcgcggac
    24001 acgcgcatcg ccgccaccgc gtcggggccg ggcagtgata gctactggcc tttgccgtgg
    24061 tatctgaacg actaccgcag cgtcggctac cacgcgcagc tccccgccga cgcctccaac
    24121 ctttccatca tcgtcggcaa cgcgacgcag gacgcgcagc tccgcgcgac cctcggcgcg
    24181 acgcaccgca aagtgggggg gacttacccg ctacgccccg gcgtcgatct cgttctctac
    24241 gcacagcgca gcctgactga caggtgatct gcatcagtgt gaccgacgcg caggagaact
    24301 gttcgttccg ggcctccggc ttcacccgcg acgggcgcgc gccctcggtt gaccgtggcc
    24361 tgacgagacg ccgcgccggg ggttggaggg cgtgccggta aagtgccggc cgccaacagt
    24421 aaagttgccg cgcttaaagt ttaagtttga caccccggcg tgtcccaaca tatagtgggc
    24481 ctctcctgcg tggtgctcta ggggctgtgc ctgacggccg tatgtccagc tcctctcagc
    24541 ctgacccttt ggaaccagac agcgaatgtt caaactccaa aagctagaga ttaccggctt
    24601 caaatccttc gccgacttca cggaactcgt cttcaccggc agcggcatca cggccgtcgt
    24661 cggcccgaac gggtgcggca aatcgaacgt ggccgacgcc atctcctggg tgctcggcga
    24721 gcagcgcgtc aagatgctgc gcggcgccga gatgaaggat gtcatctttc aaggctcgcg
    24781 caaccgtttg cctagtggga tggccgaagt cgtcctccat ctggtgcgcg acgagaccat
    24841 cgaggaagag ccggacatcg acgacatcga ctcgaccctc gaagagatag acgaccagac
    24901 cggggccatc gaggatcgac tcgcgcccga gttgttcgcc gccgcgcaag tcccggacgc
    24961 cgaggccaaa ctctccgcac ccgacgcgac cgccccggcc ctcttagctt tgtcagaacc
    25021 ctctgccgtc gaggtcacac ccggcgcgac ctccgctgtt gatgcatcga ccgtggaagc
    25081 tcccgccgaa tcggtcgaag cgcccgccgg gctggccctc gttcccgccg aaggacaacc
    25141 ggaagccgtg acgacgctgg aagcgcgcgg tgatggagaa gagactccga agcccgccgc
    25201 acagacgcac cacaagcgcc actggcgccc gcgtcgcatg gagcttgact tcgcccccgg
    25261 cgagaccgtc accgtcacgc gtcgcctcta ccgctcgggc gagagcgagt atctgctcaa
    25321 cggtcgcgtc tgccgtctgc gcgacattca agacctcttt tccggcacgg gcctttccgg
    25381 cgcgcactac gccatcatcg agcaggggcg catcggccag attctctcgg ccaagccgat
    25441 ggatcgccgc acgctcatcg aggaggccgc cggcatcacg aagttccgcg tccgccagcg
    25501 cgccgccgag gcgcgtctcg aaggcgcgcg ctcgaacctc caccgcgtct ccgacatcat
    25561 cagcgagatc gaccggcagg tgaacagtct gcgccggcag gccggcaagg cccgccgcta
    25621 cagcgccctg cgcgacgagc tgcgcgacct tctgcgccac acctacgccg ccgaagaccg
    25681 cgccctcggc acgctcctgg aaaccctgcg cgcgcagatc accgacgcct ccgaagaggc
    25741 cgacgcgctt tcgtcaaaac tctccgccgg cgaagacgag gcccgccgcg ccacgtccga
    25801 ggcccgcacg cgcgaggaag aattagccga gacgcgcgcc gccgtcgccg aatcgactct
    25861 caggcaagac cggcgcgagc gcgagcgcgc ctatcaggaa gaacaggccg acgccctcca
    25921 taagcgcgcg gccgaagtgc gacaggagat cgacactttg cgcgggcgtc tctcggccat
    25981 cgagcaggaa ggagagggct tgcgcctgtc cgacgcgcaa ctacgtgagg agagcgacgc
    26041 ctccgccggg ctgttagctt ccgccgaggc cgcctacgcg cagcgcctcg cggaagtggc
    26101 tgaggccgag gccgtcatcg aggtggcgcg ggctgaactc ctcacgcaca cggccgtcgt
    26161 cgagcgcctg cgcgagatcg gacgccagct cgaagcggcg ctcgaccgtg tggccatgca
    26221 ggccgagggt ctcgcgcgcg agggcgagcg cgcggccgtc gcgcacgaac agtcgcaggc
    26281 gcaagcccaa attttgaacg aacagattcg cgtcgcgcgc gcgcaactgg aaagtctcgt
    26341 cgccgagcgc gaggagtcgg cgcgcctcat cgcggaggcg cgcgtgcgcg ttgcggcgac
    26401 ctcggagcga cacgcgcgcg tgcgcgacga ggcggcgcgc gtccgtcacc gcctcgacac
    26461 gctcgaagaa atcgacacgc aacgctcgct ctacccgcaa gccgtccagc gcatcttctc
    26521 cgacgcggcc gaaggcgaag ctattagaga cttccgcgcc atcggcacgc tcgccgacgt
    26581 gctgcgcgtc gagccgcact gggagccggc cgtcgagagc gtcttcggca cgctcctcca
    26641 gtccgtcatc gtgccgacgc ccgacgacgc cctgcgcgcc gccgcgtggc tcgaagtcgc
    26701 gggcgcgggc cgcgccaact tcctcgttgc cggcctgcat ggcggctcgg agtcggacga
    26761 ggagcaagtc gagcagatcg aacaactcgg gcaagccgag acggagcagc cgcggcaatc
    26821 gctcgccgag agcgttctcg ccgaaggcgt ggagctgcgc gcgtggaacc ttctgggcgc
    26881 gccgcgcgag atcgcggccg tgttgcgccg gacggtgcca cgcgagatga acgcgcgcgt
    26941 cgtgcgcgac ctcgaagggg ccgtcgagct ttcgctcacg tccgaggaga tgtgcgtgac
    27001 gccggccggc gagtgggtca tcggcggcca gctcgtcgcg gcgggtgggc cgcccgtggc
    27061 gggcgagggc gcggggctgc tttcgttcaa gcgcgagatg cgcgaccttg gcgcgcgcgc
    27121 cgtcggactc gaagccgacc tcatcttcgc cgaggaggcc gccacggccg agcgcacgcg
    27181 cctcggcgaa ctcgaagacg catacgtcca tctgaacgaa gccatcgggc gcgatgagcg
    27241 cgagcaggtg gcgcgcgaag tcaacgccgc gcagctcacg caggaaaccg aacgcgccga
    27301 gcgtcacatg cgtgtggttg ccgacgacgc cgcgcgcctc gccgacgagc agcgcgagct
    27361 acaagagcgt cgcgaccaac agagcgcgga gaccgagtcg gtcgaggccg cgcgcgtcgc
    27421 cgccacattg gcgattgccg tggctgcgga cacacttgcc gcggcccgcc gcacttccga
    27481 ggaggcgagc gagcttctgg gccagcagcg ggccgccgcc gcggctgcct ccgagcgccg
    27541 gcgggccgcc gcatcggagc taaagcgcat ggagtcggag tcggccgacc tcgccgcgcg
    27601 gcttgagcgt tacgagtcgg aagtctccga gatgcacgcg cgtctcgaac gtctgcgcgg
    27661 ctcattggct gagatggacg agggggcagg cgaggtggaa ggcgagcgcg agcgtctgga
    27721 gctggacgtg gaagaggcgg cgcggcgcgt ggcggaggcg cgcgcgcggg ccgacgcgct
    27781 ggcgctcgaa gtgacggagc tgcatcagag tgtcgccgcc gcgcgcgacg cgcgagcagg
    27841 gctggaggtg caacgcgcgg agacgggtgc gcgtctgagc tacgtccgcg agtcgtgcat
    27901 ggcggaactc tcggcgccgc tcgaagaggt ggcgcgcgag ttcgcgcccg gtgaagagtt
    27961 cgaccttgag tcggagcgcg cccgtgtcga ggagatgcgc gcgcgtcttg agtctttcgg
    28021 cgcggtcaac atgatggcgc tcgaagagct gggcgagaac gaggagcgcc tgaagttttt
    28081 gacggagcag cgcgccgaca tcaccgacgg catcatctcc acggaggagg cgcttcggga
    28141 aatcaagcgc cgctcgcgcg agcgcttccg ccacgccttc gaggagatca atcgcaactt
    28201 cggacagttc ttccttgagc tgttcggcgg cgggcgcggt gagatgagca tgatcgacgc
    28261 cgacgacgtt ctggagtcgg gcatcgacat cgtcgcgcaa ccgccgggca agcgtctcca
    28321 gaacgtgctg ctgctttcgg gcggcgagaa ggcgatggcg gcgctcgcgc tcgtgctcgc
    28381 cgtcttccgc taccggccgt cgcccttctg cctcctcgac gaggtggacg cgccgctcga
    28441 cgaggccaac gtcggccgct tcacgacgaa gatcaaggag atgtcgcagg agacgcagtt
    28501 catcgtcgtc acgcacaaca agcggacgat ggagatggcg cgcgccctct acggcgtgac
    28561 gatggaggag gcgggcatct cgcgcctcgt ctccgtgagg ttcgagtgag catgtgggtt
    28621 aaggtcttag gcttcacctt cgcggccgcg ctcgtctcga cgggcgcggc cttccccgta
    28681 caggcgcaat ccccgcgcaa ggcgacgcct cggccgacgc cgacgcccgc ggtcgaacag
    28741 cagcaaccga agaaggtctc gcagcggccg gaggacggga aggggactga ggaggcgccc
    28801 gcgtcggtgc gcgcgggcgt ccccgcgaag ccgaaggaag acccgaacgc cgcgcactac
    28861 gtctacgagt tcaagcagcc ggacttcttc gtctacttcg tacgcatcga
//
LOCUS       DASVES010000303         4989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_551, whole genome shotgun sequence.
ACCESSION   DASVES010000303 DASVES010000000
VERSION     DASVES010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VF240_12230"
     CDS             complement(<1..290)
                     /locus_tag="VF240_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7176019.1"
                     /translation="MSTNERMQAVPEAEAAAARARDAQADARDGAAKTEELVRVRGLF
                     KHFPVEGSDDVVRAVDGLTFDIFRGETLGLVGESGCGKSTVGRCLLRLIEPTR"
     gene            complement(360..1385)
                     /locus_tag="VF240_12235"
     CDS             complement(360..1385)
                     /locus_tag="VF240_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176020.1"
                     /translation="MPHLLEVKNLQTHFPTRSGLVRAVDGVSFHVGEGELLGLVGESG
                     CGKSITALSVMRLIAAPGKIVGGEVWFDGENLLKASDARMREIRGDDIAMIFQDPMTS
                     LNPVYTVGEQIAEALRLHRRMNRRQAREAAVEAMKEVSIPDPGRRADDYPHQLSGGMR
                     QRVMISMALACDPKLLIADEPTTALDVTIQAQILELIDELRRTRNLAVLLITHDLGVV
                     AEVADRVAVMYTGRIVEESPVEELFARPKHPYTEGLLRSVPKLTEAEVKKIDRLQTIE
                     GTVPKPTDLPPGCHFAPRCQHRMPRCTEGDIPLYELDGGVRVRCVLYDLAAAVAADHQ
                     IQVISDE"
     gene            complement(1443..1907)
                     /locus_tag="VF240_12240"
     CDS             complement(1443..1907)
                     /locus_tag="VF240_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEX7176021.1"
                     /translation="MKKILLSVILLTCGALYDISAHAQTTQPDAGSPEAVARGFYAFY
                     LGELNKDNWSPLKKRREALKYMTPEFHRRAPRIIQQQMVDIIICAQDWDPKWIAGLTV
                     GRAGVRGARATTVVTLPPFGGYVSPDNPPIRIKLTLVKRGSAWLVNATECLF"
     gene            complement(2343..2588)
                     /locus_tag="VF240_12245"
     CDS             complement(2343..2588)
                     /locus_tag="VF240_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HEX7176022.1"
                     /translation="MSQTLKAIYRDGAFIPQVPCDLPEGAEVELTVSAPRSLPPPVTD
                     PEERKRILEEMVARMRANPIPADAPRRFTREELHERR"
     gene            complement(2725..3081)
                     /locus_tag="VF240_12250"
     CDS             complement(2725..3081)
                     /locus_tag="VF240_12250"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX7176023.1"
                     /translation="MSAPGRLFIELYADEDVSVLAADLLRARGFSVITTRAAGQLGQS
                     DASQLAYAVEHGKTFLTHNRADFEALARQYFATGKIHSGIIIAVRRPPRELVQRLLIV
                     LNQITADEMVNQLRYV"
     gene            complement(3078..3344)
                     /locus_tag="VF240_12255"
     CDS             complement(3078..3344)
                     /locus_tag="VF240_12255"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX7176024.1"
                     /translation="MTTATGHRYIVADDEILGGEPIIKGTRTPVRAIVEMWRQGVAPE
                     EIGEHLPHLTLAQVFDALSYYSDHTDEINAHIERNRIPEGLLHP"
     gene            complement(3509..3979)
                     /locus_tag="VF240_12260"
     CDS             complement(3509..3979)
                     /locus_tag="VF240_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEX7176025.1"
                     /translation="MKNATRNTLLLALLLLACGVLLAPPAAAQTANAAAPPAPAPEAV
                     VRNFYAWYLGQLNKENWEPLKNRREALKYLTSEFHKRIPRLEEDLMADIIICAQEWEP
                     KWATDYTVGRATIRGAKASTVVRLPVGNDTAIKIKTILVQRGGAWKIDGTECVN"
     gene            complement(3976..>4989)
                     /locus_tag="VF240_12265"
     CDS             complement(3976..>4989)
                     /locus_tag="VF240_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7176026.1"
                     /translation="DTFAYCAREIPNWNTISISGYHIREAGSTAAQEVAFTLADAIAY
                     VEAAIAAGLEVDDFAPRLSFFFNAHNNLLEEVAKFRAARRLWARLMRERFHARDPRSQ
                     MLRFHTQTAGSTLTAQQPEINVVRTTIQALAAVLGGTQSLHTNAMDEALSLPTEQAAR
                     LALRTQQVIAHESGAADTVDPLAGSYAVEQLTNEIERLASDYIDRIDALGGTLRAIET
                     GYVQREIQEAAYRYQRAVETQDAVVVGVNRFRSEEDAPVPTLRIDESVERAQIERVRR
                     LRERRDPEAARAALISLEDAAHGAENLLPRILHCVEAHATVGEISHTLRRVWGEYQER
                     TTL"
BASE COUNT          830 a         1532 c         1655 g          972 t
ORIGIN      
        1 cgcgtcggct cgatgagtcg cagcaggcag cgcccgacgg tcgatttgcc acagcccgac
       61 tcgcccacca gccccaacgt ctccccgcgg aagatgtcga acgtcagacc gtccacggcg
      121 cgcaccacgt cgtcgctgcc ctcgacgggg aagtgcttga acaacccacg cacgcgcacg
      181 agttcttctg tcttcgccgc gccgtcacgc gcgtccgcct gcgcgtcgcg cgcgcgggcc
      241 gccgccgctt cggcttcggg gacggcttgc attctttcgt tagtgctcat gccttaagta
      301 gtgatgagtg atgagtgata agtgataaga aaatcctgct tgctctttct catcacttat
      361 cactcatcac ttatcacctg tatctggtgg tctgcggcga cggccgccgc gagatcgtag
      421 agcacgcagc ggacgcgcac gccgccgtcc aactcgtaga gcgggatgtc gccctcggtg
      481 cagcggggca tgcggtgctg acagcgcggc gcgaagtgac agcccggggg caggtcggtc
      541 ggcttcggca ccgtcccctc gatggtctgg aggcggtcga tcttcttcac ctcggcctcg
      601 gtcagcttcg gcaccgagcg caacaaccct tcggtgtacg ggtgcttggg acgggcgaac
      661 aactcttcga ccggcgactc ttcgacgatg cgccccgtgt acatcaccgc cacgcggtcg
      721 gccacctcgg ccaccacgcc gaggtcgtgc gtgatgagca gcacggcgag gttgcgcgtg
      781 cggcgcagct cgtcgatgag ttcaaggatt tgcgcctgga tggtcacgtc gagcgcggtc
      841 gtcggctcgt cggcgatgag gagtttgggg tcgcaggcga gggccatgga gatcatcacg
      901 cgctggcgca tcccgcccga gagctggtgc gggtagtcgt cggcgcgacg gccggggtcg
      961 gggatggaca cctctttcat cgcctcgacg gccgcctcgc gcgcctgcct gcggttcatc
     1021 cggcgatgga gcctgagcgc ctccgcgatc tgctcgccca cggtgtagac cgggttgaga
     1081 ctcgtcatcg ggtcctgaaa gatcatcgcg atgtcgtcgc cccgaatctc gcgcatccgc
     1141 gcgtccgacg ccttcagcaa attctccccg tcgaaccaca cctccccgcc cacaatcttc
     1201 cccggcgccg caatcaacct catcaccgac agcgccgtga tcgacttccc gcaccccgac
     1261 tcgcccacca gccccaacaa ttccccctcg cccacgtgaa agctcacgcc gtccacggcg
     1321 cgcactaacc ctgagcgcgt cgggaaatgc gtctggagat ttttaacttc gagcaggtgc
     1381 ggcattttgt cgttcggggt gattacgttt tacaggattc gccgtcgctt tgccaaatta
     1441 cttcagaaga ggcactccgt cgcgttgaca agccacgcgc tgccgcgctt gacgagggtg
     1501 agcttgatcc tgatgggcgg attgtcgggg ctgacgtagc cgccgaatgg ggggagggtg
     1561 acgaccgtgg tggctcgcgc gccgcggacg cccgcgcgac cgacggtgag gcccgcgatc
     1621 cacttcgggt cccaatcctg agcgcagatg atgatgtcca ccatctgctg ctggatgatg
     1681 cgcggggcgc ggcggtggaa ctcgggcgtc atgtacttga gcgcctcgcg tcgcttcttg
     1741 agcggcgacc agttgtcctt gttcagctcg cccagataga aagcgtagaa gccgcgggcg
     1801 accgcctcgg ggcttccggc gtcgggctgc gttgtctgcg cgtgcgcgga gatgtcatag
     1861 agcgcgccgc aagttagtag aatgaccgag agcagaatct ttttcatcaa actcttcctc
     1921 ttagtgttgg ttatcacaga aggcgttacg gggaggcgaa tgggttgaca acctccagcc
     1981 cctcggcccg agcggaggcg tcgaaatctt ccgagtagag gcgcgtgatt ccgccttcca
     2041 gacacgcggc tacaatcaga gcgtacccag aacgagagaa gatatgtagc tatcagcctc
     2101 tccgccgtat caaagtttgc catgtcggca tcaccgtctg ccaggcagat cgcatgtcag
     2161 cgagatcggc taacgcctgt gactggtcgt aaccgcatcg ctcaagtttt cttgaggccg
     2221 cgacgtattc gcaagcgacc tgccacagaa gtactccgtc aacgagcgag gagagtaaat
     2281 tcgtcgctaa ggattgcttc agcgggtcgc gcgggtcacg tgcgtagatg aggatgttcg
     2341 tatcaacggc gttcatgcaa ctcctcgcgt gtgaagcggc gcggggcgtc agcaggaatg
     2401 gggttggcgc gcatccgcgc gaccatctcc tcaaggatac gtttacgttc ttcggggtca
     2461 gtaaccggcg gcggaagcga acgcggcgcg gagactgtta actccacttc cgcaccctcg
     2521 ggcaagtcac aagggacttg aggaatgaac gcgccgtcgc ggtatatcgc cttcaatgtt
     2581 tgagacatgg gcctctcctt ctcgctactt attctacacc gtcacggcct cgcggtactc
     2641 gccccagacg cagcacctgc ctttgaataa ataatctgtg atctcgaaga gtctgcccct
     2701 ctacacccgt cgtagtcgag acctctacac atagcgcaac tgatttacca tctcgtcagc
     2761 cgttatctgg ttgaggacga tgagcaaccg ctgaacaagt tcgcgcgggg ggcgacgcac
     2821 ggcaatgatg atgccggagt gaatctttcc ggtagcgaaa tattgtcggg caagcgcctc
     2881 gaagtcggcg cggttgtgcg tcagaaatgt cttcccgtgt tcgacggcgt aggccagttg
     2941 gctggcgtcg ctttggccca gttgtccggc cgcgcgcgtg gtaatcaccg agaacccacg
     3001 cgcccggagc aggtctgccg ccagcacgct cacgtcctca tccgcataga gttcgatgaa
     3061 gagtcggccc ggcgcgctca tgggtggagc aaaccctcgg ggatgcggtt acgctcgatg
     3121 tgcgcgttga tctcgtccgt gtggtcgctg taatagctga gggcgtcgaa gacctgcgcc
     3181 agggtcaggt gtggtaggtg ttcgcctatc tcttcgggcg cgactccctg tcgccacatc
     3241 tcgacgatgg cgcgcacggg cgtgcgcgtc cccttgatga tcggctcgcc gcccagaatc
     3301 tcgtcgtcgg cgacgatgta acgatgtccg gttgcggtcg tcatggcttg aagtttaccg
     3361 acaaacacgt ccaatgcaac agcgaagacg cgcccgctcg acaggattac ttttctacac
     3421 cgtcacggcc tcgcggtact cgcccagatg cggcgcagcc tgtgatacct ctggaaccca
     3481 attcttcccc gaaggttgaa gccacacgtc agttgacgca ctcggtgccg tcgattttcc
     3541 atgcgccgcc gcgctgcacg agaatggtct tgatctttat tgccgtgtca ttgcctacgg
     3601 gcagcctgac gaccgtggac gctttcgccc cgcggatggt cgcgcggccg acagtgtagt
     3661 ctgtcgccca cttcggctcc cattcctgcg cgcagatgat gatgtcggcc atcaagtcct
     3721 cttcgagacg cgggatgcgc ttgtggaact cggaggtcag atacttgagc gcctcacggc
     3781 gattcttgag cggctcccag ttctccttgt tgagctggcc caagtaccac gcgtagaagt
     3841 tgcggacgac cgcctcggga gcaggcgcag gcggcgcggc ggcgtttgcc gtctgcgctg
     3901 ccgccggggg cgcaagcagc actccgcagg ccaggagcag cagggccagc aggagtgtgt
     3961 ttctcgtcgc gttcttcata gagtcgtcct ctcctggtat tcgccccaga cgcggcgcag
     4021 cgtgtggctg atctccccga cggtggcgtg ggcctcgacg cagtggagga tacgcgggag
     4081 gaggttttcg gcgccgtggg cggcgtcttc gaggctgatg agggcggcgc gggccgcctc
     4141 cgggtcgcgg cgctcgcgca accgtcggac gcgctcgatc tgtgcgcgct cgaccgactc
     4201 gtcgatgcgg agagtgggaa cgggcgcgtc ctcttcggag cggaagcggt tgacgccgac
     4261 gacgacggcg tcctgagttt cgacggcgcg ctgatagcgg taggcggcct cctggatttc
     4321 gcgttggacg tagccggtct cgatggcgcg gagcgtgccg ccgagggcgt cgatgcggtc
     4381 gatgtagtcg gaggcgaggc gctcgatctc gttggtcaac tgctcgacgg cgtaggagcc
     4441 ggcgagcggg tcaaccgtgt cggccgcgcc ggactcgtgg gcgatgacct gctgcgtgcg
     4501 cagggcgagg cgcgcggcct gctcggtcgg cagcgacagg gcctcgtcca tcgcgttggt
     4561 gtggagcgac tgcgtgccgc cgagcacggc ggcgagggcc tgaatggtcg tgcggacgac
     4621 gttgatctcg ggctgctggg cggtgagggt ggagccggcc gtttgtgtgt ggaagcggag
     4681 catctgcgag cgcgggtcgc gcgcgtggaa gcgttcgcgc atgagccgcg cccaaagccg
     4741 gcgggcggcg cggaacttgg cgacctcttc gagcaggttg ttgtgcgcgt tgaagaagaa
     4801 ggagaggcgg ggggcgaagt cgtcaacctc aaggccggcg gcgatggcgg cttcgacgta
     4861 ggcgatggcg tcggcgaggg tgaaggccac ctcctgcgcg gccgtggagc cggcctcgcg
     4921 gatgtgatag ccgctgatgg agatggtgtt ccagttgggg atttcgcggg cgcagtaggc
     4981 gaaggtgtc
//
LOCUS       DASVES010000304         7611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_22718, whole genome shotgun sequence.
ACCESSION   DASVES010000304 DASVES010000000
VERSION     DASVES010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22718"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1097)
                     /locus_tag="VF240_12270"
     CDS             complement(165..1097)
                     /locus_tag="VF240_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7176027.1"
                     /translation="MRALTKVILAVFIATLVLAGVAGGQAGRRPGQSGGGASTASERG
                     TTLNVFAARADGADDEIAAHDLALYDGGMEQKIQSFAPDPDPARIVLLVDNSLGLRAD
                     VEKLAQATREFAYEIYEGDQVMIVGYDEAPEIVVEWTDNAEEIEKNLQLFRKRGEPRL
                     FDALATVLTEALRPLSASYNKRVVVLVADGLDRGSKTRFDDILAELQKQDVTVYALQI
                     PDRTGGALRRDRPKPNQVVKQLVEGTGGRILTVEEPREAAKSICDELRKNRYVLAYTP
                     MGASPYDDRRLLVIADDGINVRVKAQQPARIKVR"
     gene            complement(1140..1514)
                     /locus_tag="VF240_12275"
     CDS             complement(1140..1514)
                     /locus_tag="VF240_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX7176028.1"
                     /translation="MPIYEYTCRKCNAHVEVMQKISDKPLARHAGCGGRLEKEWSRSG
                     FQFKGSGWYVTDYAAKKSDAKTGEAKEGKEAKESKESAPAEKSESSSSKSESSAEAPK
                     ESAKAGAEKPKGKKGKDTPAGA"
     gene            2025..2666
                     /locus_tag="VF240_12280"
     CDS             2025..2666
                     /locus_tag="VF240_12280"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7176029.1"
                     /translation="MHYTRHIPLYRSFKFLSFAFIALFTLASFTDTSLTRAIAPAVEA
                     SAAAIDLENFIPADIPSSGDRDLDKIIYRAGLDYGVDPRLLHAVIWQESKYKPSAVSH
                     AGARGMMQLMPATAKRFKCEDTTDPGQNIKAGTKYLRWLLKRFDGDVVLALAGYNAGE
                     GSVDKHNGVPPFNETQKYVRIITGRYGKSRHPVYTPEVARVEFRLLPQVAQAE"
     gene            2934..5057
                     /locus_tag="VF240_12285"
     CDS             2934..5057
                     /locus_tag="VF240_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7176030.1"
                     /translation="MSTQLPMPTTAQTLSLERKIRMHEAREAIINRLAPDIHSEIDLD
                     RFLRAIVAELGRMMEADRCDVIQIVEGVELRISHEWREGEDVPSSLGVSIPVDFAQLS
                     ERFDLTQPIRIDDTSPHGTDARLGLLPASLGTRSLLVVPVVLDGRVLGLLGLHMTRSV
                     RLWLDEEVRFLQSIARQIAVGYQYARLYTDTQREASRTRALLEIANIINARSDFGEVT
                     SHVLERAVSLVGADYCALGVVEPSGKSISLAAFKAAPHSTTSGVRQLIEAHGRSIDIT
                     AFPAITDLLAEGKTLTLLDTTLPPPVRFVFNSTLGGRAALIAPVRIGEQTFGLLGFVW
                     GGERVSFDEHDISLVEGIALQIGTALERDQLSAEVMRLRSVLHERHAEDRIIGQAPSI
                     RRAIELALNVADTQTTVLIQGESGTGKELLANLIHFNSKRRDRPYIKLNCGAIPETLL
                     ETELFGHERGAFTDARARRRGRFEEADGGTLFLDEIGEMSPSAQVRLLRVLQDGEYTR
                     VGGGDVLHADVRVLAATNVDLERAVEEGSFRRDLFYRLSVFPVTLPPLRERPGDVQPL
                     VIHFLEHYKQKTGRFISGISKQALTALTGYDWPGNVRELENAVERAVIIASGRQIELD
                     DLPEAIGRVVREERASTRRERERAASEGRRVKLEIEVPATVEEIERRAIEATLDYTGG
                     DKTRAARALGIGRKTLYRKLKEYEG"
     gene            5312..5719
                     /locus_tag="VF240_12290"
     CDS             5312..5719
                     /locus_tag="VF240_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX7176031.1"
                     /translation="MSQQLQRRRERESDLSKQTACFVLGGVVGAVVALLLAPKSGREL
                     RTDLADVTRKGVDRTRETAAVARERAGEYYDVASQRAGELAGSVREAASRRGEHLSAA
                     IDAGKRAYLDEKRRTESGAIEAAPTYYEEGGKG"
     gene            5733..6290
                     /locus_tag="VF240_12295"
     CDS             5733..6290
                     /locus_tag="VF240_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX7176032.1"
                     /translation="MFLAFQMSANDPAFWTMMMTIITAVSFVVIAVAMTVIALAARRV
                     VTTVGRVEQRVEPLIGRVGALTEQVREVAEQVQLMSGHLTTATMHFSESMALIKEEVR
                     ELKEVVGLSAETARDHVERISRSIDQTHEQLMGTTVFIQSKLVAPAREMAAIMAGVRR
                     GLEVLVAPSPKSIDQTYAEDEMFIG"
     gene            6533..>7611
                     /locus_tag="VF240_12300"
     CDS             6533..>7611
                     /locus_tag="VF240_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7176033.1"
                     /translation="MSTEAPLDLKKTVNLPKTNFSQKANLGQTEPARLKKWTEMGLYQ
                     LIRRARSGRDKFILHDGPPYANADIHLGTALNKVLKDLVVKSRTMMGYDAPYVPGYDC
                     HGLPIELHVERKLGEKKRLMPPASVRRACREFAADALKRQTRDFQRLGILGLWEQPYL
                     TMSNRYEAETARLFGRFVERGYVYKGARPVYWCIHDQTALAEAEVEYNEHTSPSVYVK
                     FPAAFDPVTIDPALAGRKVFFLIWTTTPWTLPANLGIAVNPLFEYAAIENPENGEVYI
                     VASELAAEVARKCGIGEQRQVYFGAGMFSDPEELRFKVLARFSGALLDRLEARHAWLD
                     RPSLLMLGDHVTLGGESDGETELDL"
BASE COUNT         1376 a         2544 c         2313 g         1378 t
ORIGIN      
        1 gcgcagctcg aagtgctgct cgtcctcgtc gtcgtcgtga tgttctacat ttacggcagg
       61 aaaaaaccct gacccgttaa aagccgtgaa tcgtcaatcg tcaatcgtca atcgaagaag
      121 gcagtttcga ttgacgattg acgattgacg attcacggct ttttttatct gaccttgatg
      181 cgcgccggct gctgcgcctt gacgcgcacg ttgatgccgt cgtcggcgat gacgaggagg
      241 cggcggtcgt cgtagggcga ggcgcccatg ggcgtgtagg cgagcacgta acggttctta
      301 cgcagctcgt cgcagatcga cttggcggcc tcgcgcggct cctccacggt gaggatgcgc
      361 ccgcccgtgc cttcgacaag ttgcttgacg acctgattgg gcttgggccg gtcgcggcgg
      421 agcgcgccgc ccgtgcggtc ggggatttgc agagcgtaga cggtcacgtc ctgcttctgt
      481 agttcggcga gtatgtcgtc gaagcgcgtc ttgctgccgc ggtcgaggcc gtcggcgacg
      541 agcacgacga cgcgcttgtt gtaggaggcc gagagcgggc gcagggcttc agtgaggacg
      601 gtcgcgagcg cgtcgaacag acgcggctcg ccgcgcttgc ggaagagctg gaggttcttc
      661 tcgatctcct cggcgttgtc cgtccactcc acgacgatct ccggcgcctc atcgtagccg
      721 acgatcatca cctgatcgcc ctcgtaaatc tcgtaggcga actcgcgcgt cgcctgcgcc
      781 agcttctcca cgtcggcgcg caagccgagc gagttgtcca cgagcagcac gatgcgcgcc
      841 gggtcggggt cgggcgcgaa gctctgtatc ttctgctcca tcccgccgtc gtagagcgcg
      901 aggtcgtgcg cggcgatctc gtcgtccgcg ccgtcggcgc gcgcggcgaa gacgttgagc
      961 gtcgtgccgc gctcggacgc cgtcgaagcg cccccgcccg actgccccgg acgacgaccg
     1021 gcctgcccac ccgccacccc cgccagcacg agtgtcgcga tgaagaccgc gagaataact
     1081 ttggtgagcg cgcgcataaa atttgttcgc ccccccggcc gctcgtcgag tcagaactct
     1141 caggcgcccg caggcgtgtc tttccccttc ttacccttcg gcttctccgc ccccgccttc
     1201 gcgctctcct tcggcgcttc cgctgaggac tcgctcttcg aagatgaaga ttcactcttc
     1261 tccgccgggg ccgactcctt agactccttc gcctctttcc cttccttagc ctccccggtc
     1321 tttgcgtccg acttcttggc agcgtagtcg gtcacgtacc agcccgagcc tttgaactgg
     1381 aacccgctgc gcgaccactc cttttcgaga cggccgccgc acccggcgtg gcgagcaagc
     1441 ggcttgtcgg aaatcttctg catcacttcg acgtgcgcgt tgcacttccg gcaagtgtat
     1501 tcgtaaatgg gcatcggctt cggtttcgga cctctttgga taagcttcta tttatacgtc
     1561 ggcaaattat acgaaccagt ttttttgctt gcaaccgcgg ccaacatttg atgcaaagtt
     1621 tggcagcggg cgtgcgcccg cgcagcgtgg ggtctgaaga aaaatctctc gtgagcgaag
     1681 ttttttgtct cgcttcggaa cgcatccgaa gttgaaccga acctcaaaca tggttcagga
     1741 tgaacgaacc gggagccaag ccgctacgtg tcagcacctt acctatggtt gatttaaata
     1801 cgtacaagaa aaggacttag gcggctctat ttaatgcgcc cgtgcaaccg gaagtgtctc
     1861 tcatccatca tactcccggt ttcgataaag gctgctcttt ggcagacatg atcgcttcca
     1921 ccatgcgtag cccccaagct atgcgctcgc ctcgcaagga ttgtgagcgc aactgaagat
     1981 ggctcccgag agggtcaccc gaagcacatg gagaacgaat caggatgcac tatacccggc
     2041 atattcccct ctatcggtct tttaagtttc tgtcttttgc attcatcgct cttttcacac
     2101 tggcgagctt caccgacacc tctctgacgc gcgcgattgc gcccgccgtc gaagcgagcg
     2161 ccgccgcgat tgatctggag aacttcatcc cggccgatat cccctcttcc ggcgaccgcg
     2221 acctcgacaa gatcatctac cgcgccggcc tcgactacgg cgttgacccg cgcctcctcc
     2281 acgcggtgat ctggcaggag tcgaagtaca agccctcggc cgtctcccac gccggcgcgc
     2341 gcggcatgat gcagctcatg ccggccactg ccaagcggtt caagtgcgaa gacacgaccg
     2401 acccgggcca gaacatcaag gccggaacga agtacctgcg gtggctcctc aagcgtttcg
     2461 acggcgacgt ggtgctcgcg ctcgccggct acaacgcggg cgaaggctcg gttgacaagc
     2521 acaacggcgt gccgcccttc aacgagacgc agaagtacgt ccgcatcatc acgggtcgct
     2581 acggcaagtc gcgccacccc gtctacacgc ccgaagtggc gcgcgtcgag ttccgcctgc
     2641 tgccgcaagt cgcgcaggcc gaataacgaa atctaagagg tcagaggccg gaggttagag
     2701 gtcagtgaaa agacaatgtg tcttcactga cctctaacct tttgcctccg acctctttcc
     2761 ctgctttgca acgcggggcg cgcttcgata tgatttgcgc ccggttgtca aagaccctcc
     2821 ggcgcgcgcg agccggccgg cacggccgtg ttctctcctc gaactcttcc accgacattt
     2881 cgaagcgcgc cggactttca tccggggaaa gttaaggaga ggaaacggac gctttgtcca
     2941 cgcaactgcc catgcccacc accgcgcaga cgctctctct ggagcgcaag atcaggatgc
     3001 acgaggcgcg cgaggccatc atcaaccgcc tcgcgcccga catccattcg gagatcgacc
     3061 tcgaccgctt cctgcgcgcc atcgtcgccg aactcggccg catgatggag gccgaccgct
     3121 gcgacgtgat ccagatcgtc gagggcgtcg agctacgcat cagccacgaa tggcgcgagg
     3181 gcgaagacgt gccgtcgagc ctcggcgtct ccatccccgt ggacttcgca cagctctccg
     3241 aacgcttcga cctcacgcag cccatccgca tcgacgacac ctcgccgcac ggcacggacg
     3301 cgcgcctcgg cctcctgccc gcctccctcg gcacgcgctc gctcctcgtc gtccccgtcg
     3361 tcttagacgg ccgcgtgctc ggcctgctcg gtctccacat gacgcgctcc gtgcgcctct
     3421 ggcttgatga ggaagtcaga ttcctccagt ctatcgcccg ccagatagcc gtcggctacc
     3481 agtacgcgcg tctctacacg gacacgcagc gcgaggcgag ccgcacgcgc gcgctcctgg
     3541 agatcgccaa catcatcaac gcccgctccg acttcggcga ggtgacctcg cacgtccttg
     3601 agcgcgccgt ctcgctcgtc ggggccgact actgcgccct cggcgtcgtc gagccgtcgg
     3661 gcaagtccat cagcctcgcc gccttcaagg ccgcgccgca ctcgaccacc tctggcgtgc
     3721 gccagctcat cgaagcgcac ggccgctcca tcgacatcac ggccttcccc gccatcaccg
     3781 acctgctcgc cgagggcaag accctgacgc tcctcgacac gacgctcccg ccgcccgtcc
     3841 gcttcgtctt caactccacg ctcgggggcc gcgccgcgct catcgcgccc gtccgcatcg
     3901 gcgagcagac tttcgggctg ttggggtttg tctggggcgg cgagcgcgtg tcgtttgacg
     3961 agcacgacat ctcgctcgtc gaaggcatcg ccctccagat cggcacggcg ctcgaacgcg
     4021 accagctctc agccgaggtg atgcgcctga gaagcgttct tcacgagcgc cacgccgaag
     4081 accgcatcat cgggcaagcg cccagcatcc gccgcgccat cgagctggca cttaacgtcg
     4141 ccgacacaca gaccaccgtc ctcattcagg gcgagagcgg gacggggaag gaactcctcg
     4201 ccaacctcat ccacttcaac tccaagcgac gcgaccgccc atacatcaaa ctcaactgcg
     4261 gcgccattcc cgagacgctg ctggagacgg agctgttcgg acacgagcgc ggggcgttta
     4321 cggacgcgcg cgcgcgccgg cgggggcgct tcgaggaggc ggacggcggc acgctctttc
     4381 tcgacgagat tggtgagatg agtccgtcgg cgcaggtgcg tctgctgcgc gtccttcagg
     4441 acggcgagta cacgcgcgtc ggcggcggcg acgtgttgca cgcggacgtg cgtgtgctcg
     4501 ccgcgacgaa cgtcgatctg gagcgcgccg tcgaggaagg cagtttccgc cgtgacctct
     4561 tctaccgcct gtcggtgttc cccgtcacgc ttccaccttt gcgcgagcgg cccggcgacg
     4621 tgcagccgct cgtcatccat ttcctcgaac actacaagca gaagaccggc cgcttcatct
     4681 ccggcatctc gaagcaggcg ctcacggcgc tcacgggcta cgactggccc ggcaacgtga
     4741 gggaattgga gaacgccgtc gagcgcgccg tcatcatcgc ctcgggccgg cagatcgaac
     4801 tcgacgattt gccagaggcc atcggccgcg tcgtgcgcga ggagcgagcc agcacccgcc
     4861 gcgagcgcga gcgggccgcg agcgagggcc gccgcgtgaa actggagatc gaggtgcccg
     4921 ccacggtcga ggaaatcgag cgccgggcca tcgaggccac actcgactac acgggcggcg
     4981 acaagacgcg cgccgcccgc gccctcggca tcggccgcaa gacgctctac cgcaagctca
     5041 aggagtacga aggatgatgg atgagggcaa gggggaaagg ggaagggtta aagggtaaag
     5101 ggtaaaagta aggcggaagc cagcatcttt cattgtcctt cacttttgcc tttgcccctt
     5161 accccttccc ctttcccctt tgccttttgc cttcaatcat tgcaccgctt cgcgcgcttt
     5221 tgttatagtt tgcgaagcgc ttaagagact ttgttcatac taaatgtgcc ggggaggccg
     5281 gtgggtttgc ttccccgcga aagaggtcgc catgtcacag caattacagc gcaggcgtga
     5341 gagggaatcc gacctgtcga agcagaccgc gtgcttcgtt ctgggcggag ttgtgggggc
     5401 ggtcgtggcg ttgctgctcg ccccgaagtc ggggcgcgag ctgcggacgg acttggccga
     5461 cgtgacgcgc aagggcgttg accgcacgcg cgagacggcc gccgtggcgc gcgagcgcgc
     5521 cggggaatac tacgacgtcg cctcgcagcg cgcgggcgaa ctcgccggct cggtgcgcga
     5581 ggccgcgtcg cggcgcggcg agcatttgtc ggccgccatc gacgccggca agcgcgccta
     5641 tctggatgag aagcgccgca cggaatcggg cgccatcgag gccgcgccca cttattacga
     5701 ggaaggcggc aaaggctgaa gccccacacg atatgttcct cgcattccag atgagcgcga
     5761 acgacccggc gttctggacg atgatgatga cgatcatcac ggccgtctcc ttcgtcgtca
     5821 tcgccgtcgc catgaccgtc atcgcgctgg cggcgcggcg cgtcgtcacg acggtgggac
     5881 gcgtcgagca gcgcgtggag ccgctcatcg ggcgcgtcgg cgcgctcacc gagcaggtgc
     5941 gtgaggtggc cgagcaggtg caactgatgt cggggcacct gacgacggcc acgatgcact
     6001 tctccgagtc gatggcttta atcaaagaag aggtgcgcga actcaaggag gtcgtcggcc
     6061 tctcggccga gacggcccgc gaccacgtcg agcgcatcag ccgctcgata gaccagacgc
     6121 acgagcagtt aatgggcacg accgtcttca tccagtcgaa gctggtggct ccggccagag
     6181 agatggcggc gattatggcg ggggtgcggc gcggtctgga agtgctcgta gccccctccc
     6241 cgaaatcaat cgaccagacc tacgcggagg acgagatgtt catcggctga aaatctccga
     6301 agccttaacc agagcgcgac gcgcgcccgg gccaaaatct ccgcaagcga gacgacggcc
     6361 cgggcgtttt atttgaagta agcagtgagc agtgagcagt aagcagacag agctattcgg
     6421 cgacgtgcga cgagctgcca aagcgaaaaa aactgctcac tgctcactgc ttactgctca
     6481 cttctcacta tgctagtatt catatttcac cgaaggaagt gaccgcaaga ccatgtcaac
     6541 cgaagcaccg cttgatctta agaagaccgt caatctgccg aagacgaact tctcgcagaa
     6601 ggccaacctc gggcagacgg agccggcgcg tctgaagaag tggacggaga tgggcctcta
     6661 ccagctcatc cgccgcgcgc gctcgggccg cgacaagttc atcctccacg acggcccgcc
     6721 ctacgccaac gccgacattc acctcggcac agcgctcaac aaggtgctca aggatttggt
     6781 cgtcaagtcg cgcacgatga tgggctacga cgcgccgtat gttccgggct acgactgcca
     6841 cggcctgccc atcgagctgc acgtcgagcg caagctcggc gagaagaaac gtctgatgcc
     6901 gcccgcgagc gtgcgccgcg cctgccgcga gttcgccgcc gacgccctca agcgccagac
     6961 gcgcgacttc cagcgcctcg gcatcctcgg cctctgggag caaccctacc tgacgatgtc
     7021 gaaccgctac gaggcggaga ccgctcgcct gttcggccgc ttcgtcgagc gcggctacgt
     7081 ctacaagggc gcgcgccccg tctactggtg catccacgac cagacggctc tggccgaggc
     7141 cgaggtcgag tacaacgagc acacaagccc gtccgtctac gtcaagtttc cagcagcgtt
     7201 cgaccccgtg accatcgacc cggcgctcgc ggggcgcaag gtcttctttc tcatctggac
     7261 gacgacgccg tggacgctcc ccgcgaacct cggcatcgcc gtcaacccgc tcttcgagta
     7321 tgccgctatc gagaacccgg agaacggcga ggtctacatc gtcgcttcgg agttggccgc
     7381 agaggtagct cgcaagtgcg gcatcggcga gcagaggcaa gtttatttcg gggcaggaat
     7441 gttttccgac cccgaggagt tacgatttaa ggtgctcgcg cgtttctccg gcgcgctgct
     7501 cgaccgcctc gaagcacgcc acgcatggct cgaccggccg tcgcttttga tgctcggcga
     7561 ccacgtcacc ttgggcggcg agtcggacgg cgagaccgag cttgatctga a
//
LOCUS       DASVES010000305        13154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_855594, whole genome shotgun sequence.
ACCESSION   DASVES010000305 DASVES010000000
VERSION     DASVES010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855594"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VF240_12305"
     CDS             <1..1593
                     /locus_tag="VF240_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX7176034.1"
                     /translation="DKYSELLTQDRVRVGQSEDCDLRLRRSTLPDGTGCLIELARTNG
                     HYRVAEFDPNYRLLHNGEPLLPGAEIGDGDEVLVEDCGLSLRLFPVRDLPARRRQETY
                     VAPFIESAALESAATPRRDDAKVFLREFSRELLREINTSTKIALFLIAAAFVGGILYL
                     GSAANRELKRSRDLIDKQNERLAQMQGVFEQQQERQHELDKTNQGIINTLSLAPQIRS
                     AYGGGVGLLTGSYILVERGTGRQLRYPDPGKPAADPANPAGAEELQPVLTPDGYGEPF
                     EKEFAGTAFHVGDGYVITNRHLAVEPWRADQSAQIIGASVNAQFRLTRLVVYFPGVTQ
                     PFPLRVKNTSARDDLAVCLIEGRELPKTVPALPLDKESDAAGVGRTVVMMGYPTSEER
                     ILATLPEAESLSIQQRFGSSLGLLLQHLAERNFIRPLTTQGHITDLESQRLVYEGSSA
                     EGASGAPLFGQSGRVIGVNFGMFTQMQNFNFAAPIRFALPLLQKSGWKRDETVPDANA
                     AATANAPTPKDSRPVTSTNQSR"
     gene            1750..3456
                     /locus_tag="VF240_12310"
     CDS             1750..3456
                     /locus_tag="VF240_12310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7176035.1"
                     /translation="MSASTTDVRTARITPELFPEQITRASSVLLVASLSVLVLFGFGL
                     RAYRLDAEGLSEDELNKVRAVADYRQRGLTPTNGEHPFLMKALLTVSIVAAESWNRTA
                     VAESRPHLRVSTETAARLPGAFLGALSSVLIFLLASELFGAGAGLLAAALWAFDPAAI
                     GFNRIAKEDSFLVFFFILANYFWLRGQRAAERGDRNPEPFYWAAAAAFGATMASKYVP
                     HFFAITISYYYIFQGAPRTRWRLGKLRWLKFFAIMGAAFVICNPTILLPGTWHEMRVF
                     ATENRIGHDAYEFMGQLYRNQATLWLQGSPWYFYFVFMGVKMPTAVVAGFVVGLPLLF
                     RQRLGDGRYFVLFWLFFWFFPFTLLGGKFTRYFTFVLPAVHITAALGILYAGRLLARL
                     LPHRGNSEDIRTYARAALAVVVVAFSAYASVSAAPFYRLYMTALGGGARAAGAYFPHD
                     EFYDAALRDAAREVALLARPGAIVASETPELLNYYARLHGRDDLTSVSLSDREARDTL
                     RAGDLAVVARGRRYFSNDALISDLNVAEPAAVVSHGGVEAARIYVFDDALRARTSDLL
                     AH"
     gene            3757..5835
                     /locus_tag="VF240_12315"
     CDS             3757..5835
                     /locus_tag="VF240_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176036.1"
                     /translation="MGRSRKRFTSGVRARLDARRAASILLALVFSLAFLPRLNGVAAQ
                     TQAPAGQTNEPAPTAASPFRLESISVGSDAELLTVFGSLKGLRPDAGAGETDTPLVSV
                     LRDTLGDTDPENDRLRYVWPLSYTRPSAVQRVASAVPFLYTRVGGKKRAGKGELPPAV
                     IDLSATGSDVWNRFMWLGLQNLLFNPYGILVKSATSAYDRNTRAYKQAHVIRALAILS
                     LYESQSGTSSVFTPGELREIQARLMLTDKIFGGIVDDAYLERYYHNQTVAWRDTRGHN
                     WELLRQRAEVEGLYFEPLPLPDGETMHALLWVAREDLAKNRARKFNGRFLNFGSPWTD
                     NRLARWRGHTERRYFDAENRPVARDAPGARAVELIPLALYGLEHPKIPIMLVDFRDGG
                     NPKRREMSRRILEDVTRNVLAVSKFGDIHYFLGRTVLDIVTGRRGMDMNQPTRLRAYA
                     QLKLLLSLNASLDPGLREEISDRFERVSLNPLENDLEAEADFARTQYAALLDYARRAG
                     GLAAKIERDRRAELVPLEHGRFARSFFRTANVISLGLYRHREDVRGPELRNRLDEGRR
                     LAFHRRYLREVSKSTPLVEVAWDIEDVRRSLRFIAEQGARADAKTAAAAARIFARTED
                     DETRRLCLNSLYRINNETAKNELLRIYQSPAVDQQLRLLSADYLRMAVREEQRIKPAD
                     ARIIASAIGQ"
     gene            5832..8387
                     /locus_tag="VF240_12320"
     CDS             5832..8387
                     /locus_tag="VF240_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7176037.1"
                     /translation="MTRSLKTSFARGALVLVCALTLSLLASVGAHAQAKRLVLLKVDG
                     LPHDEVERFVRERDPRTGKSRLPWIDHVFYKSGTRLANFYTRGMSLSGPSWSLIDTGQ
                     HLQIKGNVEFDRYTLHSYDYLNFIPFWIANVGRERVDMPGPELLDEVGVPLLIDAYPY
                     NERFVSFQLHQRGLRWTTLQRGLQNRFTSRSPGELFSEWNIGIDARSIIFEQQERELM
                     EKLADPRVRYLDLYTTDFDHAAHHNRDRESHLVALRELDGLVGRLWMAIRKSALAEET
                     AFVLISDHGFNSNERVYSQGYNLVTLLASAAGGGHHVVTKRRVLNDYALKGIYPLISL
                     VYATTKDSFYLKGRSAEYPTALVDFDGNERASIHLRDSDLNVVHILLQQLRRKELPAD
                     VRRAATSTLFATIDRRRPDWQRTLGGLNEEVAALGRLIAKQRAAVAATPKKFTREQQD
                     AGAHNEAIRQFARLDSWLTDEREYTEFARTLSNLLALRPDGFDPSRLNDLEFIAKGAM
                     GDSNNVERLQNYVVGPAPGGLALDTTGALDLDKSFQRVNYLDLLASVAVRNNVQPGVG
                     SRPVDFVAVRVPASSLAGALANQEQPDEAVLLHAGRDTQALILSRRDNAGRLRLRYLP
                     VANLRQSADGSLTFEAVSWRAGLPLKLWEDARLDVPAGESRESWLSGWHTDLEWLRAA
                     HRTLYSTAVVGIQEQFGRHHSDGLDADSPELTPDDRLIRRFRRRQRVLVEPDLQIFAA
                     DHWNFDLRGFNPGGNHGSFFRVSTHSTLMLAGGERTGIPRAAVVEQPYDSLSFMPTML
                     ALTGQLNNGQTPVPVLWQKGFRRFPGRVIDELFQDKKDVAAPIAHSTQAGDSP"
     gene            8528..10723
                     /locus_tag="VF240_12325"
     CDS             8528..10723
                     /locus_tag="VF240_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7176038.1"
                     /translation="MKTTPISADARPVASAVEAAPKRRRRASGYVHAGAFLFGVLLLI
                     FLVRMVGLDMIFEALADVGLGFFPLLGIIGLRHLCRTFAMRAAFAREHRRFTFWQAFK
                     ARLGGEAVTFLTFTGPVLGEATKAALLRKSVSLAARVQGLLIDNLLYNLSVALFISSG
                     ACVLLVTRDLPDAARYPVVGIAAGMMLMLVIVSAAVVRRASPATKAVNALIRLGFKRR
                     ALIHRRKHIQHVESNVYNFFRRRPAALFTVVGFDFLSHAFSVLEVWLALRMLGFDTRA
                     EVPYIIESLTKIINLVFGFVPATIGVYEGGTGFILRTLGYTTAAGVTLGIVRKAGMIV
                     WAAVGLFALMSHAGSGAWGKLVGRSPRLKTLMDNLVLSNIAHRPARTFVSVLGVAVGV
                     LLIVFTVGLAHGVLRERGRREAGVGAELMVRAAGTLGLSGAQPFGLEVGRAAEIAQVE
                     GVRAAVAVGQNTVGSDRGFGMRVIDGIPFDAYTQLSGLKIIEGRHLAERGEEAIVDAV
                     WKAERGIKVGDLVQLYERDFTIVGVYEPPGGGRIKIPLATMQEQVGAEGRCTAVLVSC
                     RDPAQQDAVAERIHERFPDDQIIFMRDLPELYTTSVPALNVFISVIVGVAASISLLVI
                     LLAMYTTVTERTRQIGVLKSLGMQNRSIAWVIEQEALIVSTLGVVLGLLLTLAARFVV
                     MRTTSLTVDIEPRWLLISLAIGLVGGTLGALYPALRAARQDAVEALSYE"
     gene            complement(10969..12891)
                     /locus_tag="VF240_12330"
     CDS             complement(10969..12891)
                     /locus_tag="VF240_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX7176039.1"
                     /translation="MSKKSVSGRLAMGAILFSLIANATAPAASGFYETPVGVNEPAVI
                     RAAPAAPVISDAERQAELAGRRARVAEKIGPKSILVMFSAEPRVYTNDVDYEFRQENN
                     LYYLTNLRQAGATLVMAPGNAGPREILFLPRRNPAAETWTGHMYSAEEARKTSGITEI
                     WEAKEFEPFMQSLRTRRAYRPADANLLMSSEATAATGTSQPVTPPVPKAEQTAAAAGA
                     ATPARPVSSSAPANLVTANAPSRGSTPTAGAATGTPVAASAAATTEPAPATLKSLFDA
                     MNQNDGALYLVLPTRNDPREYRQEQEYAANWAKAATGLNVRTAWPIFVELRSKKSPME
                     LRLLQHAIDISIEGHGRAQAVAPRSKWEYEVEAEIDYTYKRRNADNWGYPNIVGCGPN
                     ATTLHYQESQGAIRGNDLILIDSGAEYDHYTADVTRTFPASGKFSPAQAEIYNIVLAA
                     QEASFRASKPGSTIGEVHNASAEVIKDGLLRLGLITNRDSDQFRMWFMHGTSHWLGMN
                     VHDVGMRASKLEPGNVYTVEPGIYIRTDALDYLPDTPENRKFIETVRPAFEKFKGIGV
                     RIEDDVVITPEGYRNMSAALPRTIPDIEAFMAKAQKELRVSATKDDSRRRGFIFVGSP
                     LEFALSGHRHGDAGEE"
     gene            complement(12965..>13154)
                     /locus_tag="VF240_12335"
     CDS             complement(12965..>13154)
                     /locus_tag="VF240_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176040.1"
                     /translation="DEVVYEPYGLSADTYRALLGRGHKVTEKPRYMGDAQGVMIEEGT
                     NVRLGASDPRAYGEPVGY"
BASE COUNT         2139 a         4750 c         3935 g         2330 t
ORIGIN      
        1 gacaagtact ccgaattgct cacgcaggat cgcgtccgcg tcgggcagtc ggaggactgc
       61 gacctgcgcc tgcgccgctc gacgctcccc gacggcacgg gctgtctcat cgagctggcg
      121 cgcacgaacg gacactaccg cgtcgccgag ttcgacccca actaccgcct gctccacaac
      181 ggcgagccgc ttcttcccgg cgcggaaatc ggtgacggcg acgaagtgct cgtcgaagat
      241 tgtggcctgt cgctccgact cttccctgtg cgcgacctgc ccgcacgccg aaggcaggag
      301 acctacgtcg ccccgttcat cgagtcggcc gcgctggagt cggcggccac cccgcggcgc
      361 gacgacgcca aagtcttcct ccgcgagttc tcccgcgagt tgctgcgcga gatcaacacc
      421 agcaccaaga tcgccctctt tctcatcgcc gcggccttcg tcggcggcat cctctatctc
      481 gggagcgccg ccaaccgcga actcaagcgc agccgcgacc tcatcgacaa gcagaacgag
      541 cggctggcgc agatgcaggg cgtcttcgag cagcaacagg agcgccagca cgaactcgac
      601 aagacgaatc agggcatcat caacaccctg tcgcttgccc cgcagattcg cagcgcctat
      661 ggcggcggcg tgggactgct cacgggcagc tacatcctcg tcgagcgcgg gacggggcgg
      721 cagcttcgct accccgatcc ggggaagcca gcggcagacc cggcaaaccc cgcgggcgcc
      781 gaagagttgc agcccgtcct cacgcctgac ggctacggtg agcctttcga gaaggagttc
      841 gcgggcacgg ccttccacgt cggcgacggc tacgtcatca cgaaccgcca cctcgcggtc
      901 gagccgtggc gggccgacca gagcgcgcag atcatcggcg cgagcgtcaa cgcgcagttc
      961 cgcctgacac gcctcgtcgt ctacttcccc ggcgtcacgc agccgttccc tctgcgtgtc
     1021 aagaacactt ccgcgcgcga cgatttggcc gtgtgtctga tcgagggtcg cgagctgccc
     1081 aaaaccgtcc ccgcgctccc gctcgacaag gaatcggacg cggccggcgt cgggcgcacc
     1141 gtcgtgatga tgggctaccc gacgagcgag gagcgcattc tggcgacgct tcccgaggcc
     1201 gagtctctga gcatccagca gcgtttcggc tccagcctcg gactgctttt gcaacacctc
     1261 gccgagcgca acttcattcg cccgctcacc acgcagggcc acatcaccga cctcgaatcc
     1321 caacgactcg tctacgaagg cagtagcgcc gagggcgcgt cgggcgcgcc gctcttcgga
     1381 cagtcgggtc gcgtcatcgg cgtcaacttc ggcatgttca cgcagatgca gaacttcaac
     1441 ttcgccgccc ccatcaggtt cgccctcccg ctcctccaga agtctggctg gaagcgcgac
     1501 gagacggttc ccgacgcaaa cgccgccgcg accgccaacg cccccacccc caaagactct
     1561 cggcctgtga catccaccaa ccagtcgcgc tgacgcgcgg ccgtgctcgt acccaaaagt
     1621 cctgttaatg caacccagtc aggacttttg atttttgaca ccgcgccggg tctcaagcta
     1681 ctctttcgcc ttttgacgcg cgcatcccgc gccgcgctta gtctgccccc acggagagac
     1741 gcttcggttt tgtcagcatc gaccacagac gtcaggacgg cgaggatcac gccggagttg
     1801 ttccccgaac aaatcacacg ggcctccagc gtcctgctcg tcgcctctct ctccgtcctc
     1861 gtcctcttcg gcttcggcct tcgcgcctac cgactcgacg ccgaagggtt gagcgaagac
     1921 gagttgaaca aggtgcgcgc cgtggccgac tatcgccagc gcggtctgac gccgaccaac
     1981 ggcgagcacc cctttctgat gaaggcgttg ctgactgtaa gcatcgtcgc cgccgaaagc
     2041 tggaatcgga cggccgtggc cgaatcgcgc ccgcacctgc gcgtctccac tgagaccgcc
     2101 gcgcggctgc ccggcgcttt cctcggcgcg ctctcttcag tcctcatctt cctcctcgcc
     2161 tcggaactct tcggagccgg ggccggactc ctcgccgccg ccctgtgggc cttcgacccg
     2221 gccgccatcg gcttcaatcg catcgctaaa gaagacagtt tcctcgtttt cttctttatc
     2281 cttgccaact acttctggct gcgcggccag cgcgcggccg agcgcggtga ccgcaatcct
     2341 gagccgttct actgggcggc ggccgccgcc ttcggcgcga cgatggcctc caagtacgtg
     2401 ccccacttct tcgccatcac catcagctat tactacatct ttcagggcgc gccccgaacg
     2461 cgctggcgtc tgggcaaact ccgctggctg aagttttttg cgatcatggg cgcggccttc
     2521 gtcatctgta atccgacgat cctgctgccc ggcacatggc acgagatgcg cgtcttcgcg
     2581 accgagaatc gcatcggcca cgacgcctac gagttcatgg gccagctcta ccgcaaccag
     2641 gcgacgctct ggcttcaggg gtcgccgtgg tatttctact tcgtcttcat gggcgtgaaa
     2701 atgccgacgg cggtcgtcgc cggcttcgtc gtcggactcc cgctcctctt ccgccagcgg
     2761 ctcggcgacg gccgctactt cgtcctcttc tggctcttct tctggttctt cccattcact
     2821 ctgctgggcg ggaagttcac acgctatttc acgttcgtgc tgccggccgt acacatcacc
     2881 gcggccctcg gcatcctcta cgccggacgc ctcctcgccc gtctacttcc acatcgcggc
     2941 aactcagaag acatccgcac ctacgcgcgc gccgccctcg ccgtggtcgt cgtggccttc
     3001 tccgcctacg cctcggtcag cgccgctcct ttctatcgtc tttacatgac cgccctcggc
     3061 ggcggagcgc gcgccgcggg cgcctacttt ccccacgatg agttttacga cgcggcgcta
     3121 cgcgacgcgg cgcgcgaggt cgccctcctc gcccgccccg gcgccatcgt ggcgagcgag
     3181 acgcccgagc ttctcaacta ctacgcgcgt ctgcacggac gcgacgacct cacgtcggtc
     3241 tcactttccg accgcgaagc acgggacacg ctccgcgcgg gcgacctcgc cgtggtcgcc
     3301 cgcgggcgcc gctacttcag caacgacgcc ctcatctccg acctcaacgt ggcggaaccg
     3361 gccgccgtcg tctcgcatgg cggagtggag gccgcgcgca tttacgtttt cgacgacgcc
     3421 ctgcgtgccc gaacatctga ccttctcgcg cattaaaagt tataatgcaa cgtgaaaaag
     3481 ccggggcggc atcgcacatg tcttacgtac aaggggaaga gtcagacgac tttagaatcg
     3541 gcaacagtac cgcgagcgga agcctcgcgg gtctcgcggg tgcggggcgg gcctctcatc
     3601 gtaggcacac ccgctcgcta ccgctcgcgg tactgctcct gtcgcggaac gggtgcgcgg
     3661 cacgtccgtt gcagtattgt tcccatatca cgggttcgcc gccgatgcgc tcaagcgcac
     3721 actaacggcg cagcagttac gctccaggag gagtttttgg gaagaagcag gaagcgattc
     3781 acgtcaggtg ttcgcgcgcg cttagacgcg cggcgggccg cgtcgattct tctcgcgctg
     3841 gtcttctcgc tcgcgtttct gccgcgtctg aacggcgtcg ccgcgcagac gcaggcccct
     3901 gccggccaga caaacgagcc agcgccgacg gccgcgtcgc ccttccgact ggagagcatc
     3961 tccgtcggca gtgacgccga gttgctgacg gtctttggca gtctcaaggg actgcgaccc
     4021 gacgcgggcg cgggcgaaac ggacactccg ctggtcagcg tgcttcgcga cacgctcggc
     4081 gacacagacc cggagaacga ccgcctccgc tacgtctggc cgctcagcta tacgcggcca
     4141 agcgccgtgc aacgcgtcgc ttccgccgtt cccttcctct acacacgcgt cggcggcaag
     4201 aagcgcgccg gcaagggcga gctgccgccc gcggtcatcg acctctccgc gacaggcagc
     4261 gacgtgtgga accgcttcat gtggctgggc ctccagaacc ttctcttcaa tccctacggc
     4321 atcctcgtca agtcggccac cagcgcctac gaccgcaaca cacgcgccta caagcaggcg
     4381 cacgtcatcc gggcgctggc catcctctcg ctctacgagt cgcagtcagg cacgtcgtcc
     4441 gtcttcacgc ccggtgagct gcgcgagata caggcgcgcc tcatgctcac ggacaaaatc
     4501 ttcggcggca tcgttgacga cgcttacctc gaacgctatt accacaacca gaccgtcgcg
     4561 tggcgcgaca cgcgcggcca caactgggag cttttgcgcc agcgcgccga ggtcgaagga
     4621 ctctacttcg agccgctgcc gctccccgac ggcgagacga tgcacgcgct cctctgggtc
     4681 gcccgcgaag acctcgcgaa gaatcgcgcc cgcaagttca acggtcgctt cctcaacttc
     4741 ggctcaccgt ggacggacaa ccgtctcgcc cgctggcgcg gccacaccga gcgccgctat
     4801 ttcgacgcgg agaatcgccc cgtcgcacga gacgcgcccg gcgcgcgcgc cgtcgagttg
     4861 atcccgctcg cgctctacgg tcttgaacac cccaaaatcc cgatcatgct cgtggacttc
     4921 cgcgacggcg gaaacccgaa acggcgggag atgtcgcggc gcatcctcga agacgtgacg
     4981 cgcaacgtcc tcgccgtctc gaagttcggc gacattcatt acttcctcgg ccgcactgtc
     5041 ctcgacatcg tgaccggccg gcgcggcatg gacatgaatc agcccacgcg cctgcgcgcc
     5101 tacgcgcagc tcaagctgct gctctcgctc aatgcgtcgc tcgaccccgg cttgcgcgag
     5161 gaaatcagcg accgcttcga gcgcgtctcg ctcaacccgc tggaaaacga cctcgaagcc
     5221 gaagctgatt tcgcacgcac gcagtacgcc gcactgctcg actacgcgcg ccgcgccggc
     5281 ggtctcgccg cgaagatcga gcgcgaccgc cgcgccgaac tcgtccccct cgaacacggc
     5341 cgcttcgccc gctctttctt ccgcacggcg aacgtcatca gcctcggcct ctaccggcac
     5401 cgcgaggacg ttcgcggccc ggagcttcgc aaccgtctcg acgagggccg ccggctcgct
     5461 ttccaccggc gctacctccg cgaggtctcg aaatcgacac cgctcgtcga ggtcgcctgg
     5521 gacatcgagg acgtgcgccg ctcgctccgc ttcatcgccg agcagggcgc gcgcgccgac
     5581 gccaagacgg ccgccgccgc cgcacgcatc ttcgcccgca ccgaggacga cgagacgcgc
     5641 cgcctgtgtc tcaactccct ctaccgcatc aacaacgaaa cggccaagaa cgaactgctg
     5701 cgtatctacc agtcgcccgc ggtcgatcag cagcttcgtc tgctctccgc cgactatctc
     5761 cgcatggcgg tgcgcgagga acagcgtatc aagcccgccg acgcccggat catcgcctcc
     5821 gccatcggac aatgacacgc tccctcaaaa catcattcgc gcgtggcgcg ctcgtgctcg
     5881 tctgcgcgct caccctttcc ctgcttgcct ccgtgggcgc gcacgcgcag gccaagcgtc
     5941 tcgtgctgct caaggttgac ggcctgccgc acgacgaggt cgagcgtttc gtccgcgagc
     6001 gcgacccgcg caccggcaag agccgcctcc cgtggatcga ccacgtcttc tacaagagcg
     6061 ggacgcgcct cgccaacttc tacacacgcg ggatgagcct ttccgggcct tcgtggtcgc
     6121 tcattgacac ggggcagcac ctccagatca agggcaacgt cgagttcgac cgctacaccc
     6181 tgcactccta cgactatctg aacttcatcc ccttctggat cgccaacgtc gggcgcgagc
     6241 gcgtggacat gcccggcccc gaactgctcg acgaggtggg cgttcccctc ctcatcgacg
     6301 cctatcctta caacgagcgt ttcgttagct tccagctcca ccaacggggc ctgcgctgga
     6361 cgacgctcca gcgcggtctc cagaaccgtt tcacttcgcg cagccccggc gagttgttca
     6421 gcgaatggaa catcggcatc gacgcccgca gcatcatctt cgagcagcag gagcgcgagc
     6481 tgatggaaaa actcgccgac ccgcgcgtgc gctatctcga cttatacact accgacttcg
     6541 accacgccgc gcaccacaac cgcgaccgcg agtcgcacct cgtcgcgctg cgcgaactcg
     6601 acggcctcgt cggccgcctt tggatggcca tccgcaagtc ggcgctcgcc gaagagaccg
     6661 ccttcgtcct catctccgac cacggcttca actccaacga acgcgtctac agtcagggct
     6721 acaacctcgt caccctcctc gccagcgccg ccggcggcgg tcaccacgtc gtcaccaagc
     6781 gtcgtgtgct gaacgactac gcgctcaagg gcatctatcc gctcatctcg ctcgtctacg
     6841 ccaccacgaa ggattctttc tacctcaagg gccggagcgc cgagtacccc acggccctcg
     6901 tcgatttcga cggcaacgag cgcgcctcga ttcacctgcg cgacagcgac ctcaacgtcg
     6961 tccacattct cctccagcag ctacggcgca aggaacttcc ggccgacgtg cgccgcgccg
     7021 ccaccagcac gctcttcgcc accatcgacc gccgccggcc tgactggcag aggacgctcg
     7081 gcggattgaa cgaagaggtc gccgcgctcg gccgcctgat cgcgaagcag cgcgcggccg
     7141 tcgcggcaac gcccaagaag ttcaccaggg agcaacagga cgcgggcgca cacaacgaag
     7201 ccattcgcca gttcgcgcgc ctcgactcgt ggctcacgga tgagcgcgag tacacggagt
     7261 tcgcgcgcac cctctcgaac ctgctcgcac tccggcccga cggcttcgac ccatcccgac
     7321 tcaacgacct tgaatttatc gcgaagggcg cgatgggcga ctcgaacaac gtcgaacgct
     7381 tgcagaacta cgtcgtcggc cccgcgccgg gcggacttgc gctcgacacg acaggcgcgc
     7441 tcgacctcga caaaagtttc cagcgcgtga actacctcga cctgctcgcc tccgtcgccg
     7501 tgcgcaacaa cgtgcagccc ggcgtcggca gccgccccgt cgatttcgtc gccgtccgcg
     7561 tccccgcctc atctctcgcc ggcgcgctcg ccaaccagga gcagccggat gaggccgtcc
     7621 tgctccacgc cggccgcgac acacaggcgc ttatcctctc gcggcgcgac aacgcggggc
     7681 gcctccgact tcgctacctg ccagtcgcca acctgcggca gtcggcggat gggtcgctca
     7741 ccttcgaggc cgtcagttgg agagccggcc tgcccctcaa gctctgggag gacgcgcgtc
     7801 tcgacgtgcc cgcgggcgag tcacgcgaga gctggctttc cggctggcac accgacctcg
     7861 aatggctgcg cgccgcgcat cgcacgctct actcgaccgc cgtcgtcggc atacaggagc
     7921 agttcggtcg ccaccactcg gacggcctcg acgccgactc ccccgaactc acccctgacg
     7981 accgtctcat ccgtcgcttt cgccgacgcc agcgcgtgct cgtcgaaccc gatctgcaaa
     8041 tcttcgccgc cgaccactgg aacttcgacc tccgagggtt caaccccggc ggcaaccatg
     8101 gctccttctt ccgcgtctct actcactcca ctctcatgct cgccggcgga gagcgcaccg
     8161 gcattccgcg cgccgccgtc gtcgaacagc cctacgacag cctgagcttc atgccgacga
     8221 tgctcgcact caccggccaa ctcaacaacg gacagacacc cgtccccgtc ctctggcaga
     8281 aaggtttccg ccgcttcccc ggccgcgtca tcgacgagct ttttcaggac aagaaagacg
     8341 tagccgcccc catcgcccac agcacacagg ccggcgactc gccctgaagc acgccccacg
     8401 cctcagaatt tcatgcggat gaatcatcgt gttcgggttt gagaaatcac gttcgcacgg
     8461 cgcgcctgtg atagactcgc cgccatccca gccagcccct ttgattagtg agagcgcaga
     8521 taaaggcttg aagaccactc ccatttcggc agatgcccgc ccggtcgcaa gcgcggtcga
     8581 ggccgcgccc aaacgccgcc ggcgtgcgtc cgggtacgta cacgcgggtg cattcctctt
     8641 cggcgtcctg ctcctcatct tcctcgtccg catggtcggc ctcgacatga ttttcgaggc
     8701 gctcgccgac gtagggctag gattcttccc cctgctcggc atcatcggcc tgcgccatct
     8761 ctgccgcacc ttcgccatgc gcgccgcctt cgcgcgcgag caccggcgct tcaccttctg
     8821 gcaggcgttc aaggcgcgcc tcggcggcga ggccgtgacc ttcctcacct tcaccggccc
     8881 cgttctcggc gaggccacga aggccgctct gctccgaaag agcgtctcgc tggccgcgcg
     8941 cgtccagggc ctactcatcg acaatctgct ctacaacctc tcggtcgcgc tctttatctc
     9001 cagcggcgcc tgtgtcctgc tcgtcacgcg cgaccttccc gacgcggcgc gctaccccgt
     9061 cgtcggcatc gccgcgggga tgatgctcat gctggtcatc gtctcggcgg cggtcgtccg
     9121 ccgcgccagc cctgcgacga aggccgtcaa cgccctcatc cgtctcggct tcaagcgccg
     9181 cgcgctcatc caccggcgca agcacatcca gcacgtcgag tcgaacgtct acaatttctt
     9241 tcgccggcgt cccgcggccc tcttcaccgt cgtcggcttc gacttcctct cgcacgcctt
     9301 ctccgtgctc gaagtatggc tggccctacg catgctcggc tttgacacgc gcgcggaggt
     9361 gccttatatc attgagtcgc tgacgaagat catcaacctc gtcttcggtt tcgtgcccgc
     9421 caccatcgga gtttacgaag gcggtacggg cttcatcctc cgcacgctcg gatacacgac
     9481 ggccgccggc gtcacgctcg gcatcgtgcg caaggccggg atgatcgtct gggcggcagt
     9541 ggggttgttc gcgctcatgt ctcacgccgg ctcaggcgcg tggggcaagt tggtcgggcg
     9601 cagcccgcgt ctgaaaacct taatggacaa cttagtactc tcgaacatcg cacaccgccc
     9661 cgcgcgcacc ttcgtcagcg tcctcggcgt cgccgtcggc gtgttgctca tcgtcttcac
     9721 ggtcggcctc gcacacggcg tcctgcgcga gcgcgggcgt cgggaggcgg gcgtcggggc
     9781 cgagctgatg gtgcgcgcgg cgggcacgct gggcttgagc ggcgcgcagc cgttcgggct
     9841 ggaggtcggt cgtgccgccg agatcgcgca ggtcgagggc gtgcgcgcgg ccgtcgccgt
     9901 cgggcaaaac acggtgggta gcgaccgcgg cttcggcatg cgcgtcatcg acggcatccc
     9961 cttcgacgcc tacacacaac tctcgggtct gaagatcatc gagggacgac atttagccga
    10021 gcgtggggag gaggcgattg tcgatgcggt gtggaaggcc gagcgcggca tcaaagtggg
    10081 cgatctggtg cagctctacg aacgcgattt cacgatagtc ggcgtctacg agccgcccgg
    10141 tggcggacgc atcaagattc ccctcgccac catgcaagag caggtcgggg ccgaggggcg
    10201 ctgtacggcg gtgctcgtct cgtgccgcga cccggcgcag caggacgccg tggccgagcg
    10261 catccacgag cgtttccccg acgaccagat catcttcatg cgcgatctgc cggagctata
    10321 tacgacgagc gtgccggcct taaacgtctt tatcagcgtc atcgtcgggg tggccgcctc
    10381 catcagtttg ctcgtcatcc tgctggcgat gtacacgacc gtcacggagc ggacgcgaca
    10441 gatcggggtg ctcaaatcgc tcggcatgca gaaccgttca atcgcctggg tgatcgaaca
    10501 ggaggcgctg atcgtaagca ccctcggcgt cgtcctcgga ctactgctga cgctcgccgc
    10561 gcgcttcgtc gtcatgcgga cgacttcgct gaccgtggac atcgagccgc gctggctcct
    10621 catctcgctc gccatcggcc tcgtcggcgg caccctcggc gcactctacc cggccctgcg
    10681 cgccgcacgc caggacgcgg tggaggccct gagttacgaa taaacattcc gtgtacttcg
    10741 agcacaacca gccgcgtagc ggcggaagaa tgtagcccac ggcgcaagcc gtgggaacac
    10801 acgcgcaaga gaagaaagcc ccggaggggc gaaagaaaat caaggcgcct atcacgagag
    10861 cctgattttc tttcgcccct ccggggcttt tattccgtca cgtcctgtta cccacggctt
    10921 gcgccgtggg ctacattctg tcgccacctt cggtggctgc gtccactttt actcctcgcc
    10981 cgcgtcgccg tgacgatgcc cactcagcgc gaactccaaa ggcgacccga cgaagatgaa
    11041 cccccggcgg cggctgtcgt ctttcgtcgc gctcacgcgt agctccttct gggccttcgc
    11101 catgaacgct tcgatgtcgg ggatggtgcg cggcagcgcg gccgacatgt tgcggtagcc
    11161 ctcgggcgtg atgaccacgt cgtcttcgat gcggacgccg atgcccttga acttttcaaa
    11221 tgcgggacgc accgtctcga tgaacttgcg gttctcgggc gtgtccggca aatagtcgag
    11281 cgcgtcggtg cggatgtaga tacccggctc gaccgtgtag acgttgccgg gttcgagctt
    11341 cgacgcgcgc atcccgacat cgtggacgtt catgccgagc cagtgcgaag tgccgtgcat
    11401 gaaccacatg cggaactgat cgctgtcgcg gttggtgatg aggccgagtc ggagcaggcc
    11461 gtccttgatg acctcggccg aggcgttgtg tacctcgccg atggtcgagc cgggcttcga
    11521 ggcgcggaag gaggcctcct gcgcggccag cacgatgttg taaatctccg cctgcgccgg
    11581 gctgaatttc cccgaagccg ggaaggtgcg cgtcacgtcg gccgtgtagt ggtcgtactc
    11641 ggcgccggag tcgatgagga ttaaatcgtt gcccctgatc gccccctgcg actcctgata
    11701 gtggagcgtc gtcgcgttcg ggccgcaacc gacgatgttc gggtagcccc agttgtcggc
    11761 gttgcgacgc ttgtaggtgt agtcgatctc ggcctcgacc tcatactccc acttcgagcg
    11821 cggggccacg gcctgtgctc tgccgtgccc ctcgatgctg atgtcgatgg cgtgctggag
    11881 cagacgcagc tccatcggcg acttcttcga gcgcagctcg acgaagatgg gccacgccgt
    11941 ccgcacgttc aaccccgtcg cggccttcgc ccagttggcc gcgtactcct gctcctgccg
    12001 gtactcgcgt gggtcgttgc gcgtggggag cacgaggtag agcgcgccat cgttctgatt
    12061 catcgcgtcg aagagcgatt tgagggttgc gggggccggc tcggtcgtgg cggcagcaga
    12121 cgcggcgacc ggagtgcccg tggcggcgcc cgccgtcggg gtggagccgc gcgagggggc
    12181 attggccgtc acgaggttgg ccggagcgct actgctgaca gggcgcgccg gagtggcggc
    12241 gccggcagcg gcggccgtct gctcggcttt cggcacaggc ggcgtcaccg gctgcgacgt
    12301 gccggttgcg gcggtcgcct ctgacgacat caggaggttc gcgtcagcgg ggcggtacgc
    12361 gcggcgcgtg cgcagcgatt gcatgaacgg ctcgaactct ttcgcctccc agatttcagt
    12421 gatgccggaa gttttgcgcg cctcctcggc gctgtacatg tggcccgtcc aggtctcggc
    12481 ggccggattg cggcgcggca ggaaaaggat ttcgcgcggg ccggcgttgc cgggcgccat
    12541 gacgagcgtc gcgccggcct gcctcaggtt cgtcaggtag taaagattat tctcctgtcg
    12601 gaactcgtaa tccacgtcgt tcgtgtagac acgcggctcg gcgctgaaca tgacgaggat
    12661 cgacttcggc ccgatcttct cggcgactcg ggcgcgacgc cccgcgagtt ccgcctgcct
    12721 ctcggcgtcc gagatgacgg gcgccgccgg cgccgcgcga atcaccgccg gctcgttcac
    12781 ccccaccggc gtctcgtaga agccagacgc ggcgggcgcc gtggcgttcg cgattagaga
    12841 aaaaaggatg gcgcccattg ccagcctgcc tgaaacgctt ttcttactca cttcttatta
    12901 ctccttactg gccccgcacg cggggcggtt aatcttttcg ttacgaaagt tcgggtgaga
    12961 gaaatcagta cccgaccggc tcgccgtagg cgcgcgggtc gctcgcgccg aggcgcacgt
    13021 tcgtgccctc ctcgatcatc acgccctgcg cgtcgcccat gtagcggggc ttctccgtca
    13081 ccttgtggcc gcgccccaga agcgcgcggt aagtgtcggc cgaaagacca tacggctcgt
    13141 agacgacctc gtcc
//
LOCUS       DASVES010000306        23587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_991918, whole genome shotgun sequence.
ACCESSION   DASVES010000306 DASVES010000000
VERSION     DASVES010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23587
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991918"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VF240_12340"
     CDS             <1..1722
                     /locus_tag="VF240_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176041.1"
                     /translation="AETTAGPFDVAVTPLSDGRRLVAARWREESSSLRELAAGAQTFE
                     DFKTNQRKVRLLGLSTIGLMTLLLLFAATWSAIHLARGLGTPIRALAEASKRVAEGDL
                     SHRVTAIADDELAVLAANFNDMTAQLDENRRRLELNASELRETNAALDERRAYIETVL
                     ESLSTGVVSLDAQNNVTTINAAAAQMLGLDSSPQRGAPLAEVVSIDDYEMLSRVLWRA
                     RLRGRASEQTELARGRSAEGNGGQVPEGNGGQSEEGDNGNNTRPVALTATALGPRVDD
                     NRRGVVLVMEDLTELLAAQRAAAWSEVARRMAHEIKNPLTPIQLSAERIARNFQRLSD
                     AEATNGNGGASAAASGTRSSDSDEASAVFSSNGNEGGRGARERERVAGIIEECTATIG
                     REVAGLKAMVDEFARFARLPHARLAAGDLNEVVRQSVALYEDRLGDARLDVLLAPRLP
                     AALLDAEQLRRVFVNLIDNALEALEGVGDEQRVTVATAHDPARGLLLVEIADTGQGIA
                     RADFPRLFQPYFSTRGRGTGLGLAIVQRILADHGGRVRAESNRPRGARMVVELPVAEE
                     VKEEVRG"
     gene            1796..3163
                     /locus_tag="VF240_12345"
     CDS             1796..3163
                     /locus_tag="VF240_12345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7176042.1"
                     /translation="MSDSILIVDDERGIRETLRGVLEDEGFAVEAVGSGEECLKAVER
                     RAYGCVLLDVWLPEIDGLETLRRMREQGTDAAVVMISGHGNIETAVRATKLGAFDFIE
                     KPLSIEKTVLTVRNALRQRGLERAVAAYADGMRGANEMVGESVAMRALRKQIEMVGPT
                     DGRVLIYGESGTGKELVAHAIHATSRRAGAPFVEVNCAAIPEELIESELFGHVRGAFT
                     GATGAKRGKFEMADGATLFLDEIGDMSARVQAKVLRVLEEQRFEPVGSTTSMRVDVRV
                     LAATNKRLEEEIERGAFRPDLFYRLNVIPFELPPLRERLEDIPALVEHFNKKFSAAYN
                     RRPKRFEPEAVEAMRGYSWPGNVRELRNTVERVVIMHPHVKVSAADLPARGGEAPPAD
                     PLRYANFREASEALQREFIKRKLAEAEGNVSRAAELLGMDRSHLYRRMRALGINVREE
                     RAGAV"
     gene            complement(3183..6917)
                     /locus_tag="VF240_12350"
     CDS             complement(3183..6917)
                     /locus_tag="VF240_12350"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176043.1"
                     /translation="MTTTMPNPFPGLRPFESDETHLFFGRDGQSEELIRRLGQTRFLA
                     VVGTSGSGKSSLVRAGLLPALHGGLMASAGSEWRVAMLRPGHDPLGNLARTLASPEVF
                     GAPDPREAEMQTALVEATLRRSSLGLLDAVRQARAAAGGEGGRPRLAASENLLVVVDQ
                     FEELFRFRALMEVENTKEDAAGFVRLLLEAVGVPGERIYVVLTMRSDFLGDCAQFQGL
                     PEAINDGQYLIPRMTRDERREAVTGPVSVGDGRISVPLVNQLLNDMGDNPDQLPILQH
                     ALMRTWDHWLSVRRDGEPIDLPHYQAIGGMKEALSLHAEEAFGELSEPRQKIAERLFK
                     ALTEKGADNRETRRPLELGDACALVEADEAEVVAVVETFRRPGRSFLMPPPPAPLDAD
                     SLIDISHESLIRNWARLKNWTDEEAQSARIYRRLAETAVLHKAGEEALLKDPALQVAL
                     DWRERTRPNAAWARRYHPEFETAASFLDSSVAARDAEARERERQQRRDVSFKRTKLVA
                     ALLAFSVVLALGSTVYAFRQKWLASAQEARALELATQNRQLLYPADIHLAQQAFVANN
                     LQHAGELLDSHRAADDGALRFEWRYLWGVINSEKAMINFPDGSNFMALASDDQTLIVG
                     ANNQIELRDPATGAARPAPFRGLDDHRLTAVELSPDGKTLLAIGTPPDSSKPKYILLD
                     TTTGELKGTVAEIEDRPAGFAFSPDSKLLAWTTPIDGHVKLWDVNLLRETEFIKGANH
                     PDALAFSPDGKMLATGGKAPSPIILWDLGKLRSPVRYEKISECETAPPPCKITPITDD
                     VKAISFAPDGKTLMIGFSDRVVWQWHLSQNAWGDKIPATNDITSLKFSPDGKTLAIGY
                     KNGSVKVHDSVFLAHGVTLGGHQEAVRSLAFSRDGQTLFTGSKDAVRVWDVSSAVRAQ
                     LPLGSGEVREDPGEGPGRNVKSVSFSPDGKMLASVHLDSTVALWDVATRRRLEDLGAV
                     SDVDLFHFSPDGRTLAVSDAGGRVHLLDSVTRKEVWALPHERRVTALSSSPDGKLLVT
                     GDIQGYISLWEPATQTKLREVSGAPNTVKTVAFSPDGQAIAAIFHGEDAVKLWDRDLN
                     VLTELESDGVLPPPNTLAFSPDGRLLAVGLMKDFSDADPRVHFGGGMLRLWEVTGGEG
                     LRAPRELVRLRGHRDTITSLAFSPDGKTLYSSGAGGMSKLWDAISFREMVSVQDESSY
                     ATASAFSPDGRTLAAGEADGDVRLWRAATDEEIRSQSGRE"
     gene            complement(6914..8455)
                     /locus_tag="VF240_12355"
     CDS             complement(6914..8455)
                     /locus_tag="VF240_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEX7176044.1"
                     /translation="MSTGPRYADDALISYGHIDNQPLEDEERGWVDNLHERLEYRLGE
                     LLGHRPRIWRDPRLPGNVYFANELTDRLRTTCVLVSVLSPGYLQSKWCMGELVEFCRA
                     AEENGGLKVGGGRLRVFKVVKTFVERDQHPAEFQGQLGYEFYEIEQATGRPVEYGQQT
                     GRNRDQRYWDKLNDLAWDIKQTLVAASPPTTTAAVVPHVFGASKGTVYLAETSYDLRE
                     ERDRIKRELQERGYDILPNRELPYKSPEYQEAVREGVLRAKLSVHLIGASYGIIPEGA
                     SGSSVVRLQNEIAAERSGDPAFKRLIWLPERLAPQEGTQTEFIEYLKTSADPQRGAEL
                     LQTTLEELKSVIQVRLSGNGHRAAADAGDGAQAQKKIYLLCDPRDVEGIVALNDFLYE
                     RGYDVLLPLLDVGAGDDAQAIETHKENLATCDAALVYYGSANQAWFDYQRRGLEKAAA
                     QGRDAGRTPLAWGVYVGSPVSPHKQLFRTRDSFFVKNFGDFTSESLKPFLDSLGRTAT
                     DGDDQ"
     gene            8775..9938
                     /locus_tag="VF240_12360"
     CDS             8775..9938
                     /locus_tag="VF240_12360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7176045.1"
                     /translation="MDYTTRELLKRYAKLALGQPFAPVLLNILVTSVCDMRCTHCFFT
                     DELDDRPRKKLQMKTHEIQKISETLGGNLGVLILAGGEPFTRKDLPEIVRAFYENNRL
                     ESVYLMSNGQIQKRIIPDVTRILEECPNLNVAVALGIDGLKDQHDKIRQKPGSWDIAI
                     DTARQLQAIKREHPRLDIQTCTCFMNSNQDTIFDWYEYLKHDLRPDKVNFNYIRPPSA
                     DPVELDIDHARYAKLAHMIDDDSRHATIKNNYGGDAGFFKAAIDIYMHGLIAKTQEEQ
                     KAQMTCYAGTAGAVIYDEGTISSCENKTPVGNLRDHDWNFQSLWRSEAMKARRKEAAD
                     GCFCTHESNSYYPSLPFNPKHLIQIKRLEREMKKASRELERKQGEGEGLAIKA"
     gene            9960..11162
                     /locus_tag="VF240_12365"
     CDS             9960..11162
                     /locus_tag="VF240_12365"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7176046.1"
                     /translation="MFRARVPKILIISSDTGGGHRSAATAIVAGVQKFLKSDSYAVRV
                     VRALEESHHLSAKMVGVYNWVLRHRQHWMKYLYWAINRFRPETREFFYRRYVGYVREL
                     FDRWCPHVVVSVHPLTQHAFARVLKELGLAGTIPLVTVVTDPCYGFWKGWACDDVTLY
                     LVASDDARRQLIDYGISPERIKVSGMPVHPKFDLPDERAAQAARTALGLDPEKFTVFV
                     NAGWVGGGNIPEIFRELVRGDLDVQAVFLAGRNEELRAEAEGLAAGAKFPVKVIGYSE
                     HVEQIMQAANVMVSKLGGLTTFEALACRVPIIADAITKPMPQESGAATLISTRGAGVL
                     LKRANDIVPVVRRMVDDALYYAAMRKAAASVAVPDATRRIVEEIAALIPAPVMAKEEP
                     AAAEIRLA"
     gene            11284..12033
                     /locus_tag="VF240_12370"
     CDS             11284..12033
                     /locus_tag="VF240_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7176047.1"
                     /translation="MSLLLEGKRAFITGGSRGIGAALCEVFAREGADVAFNYHSRDEL
                     ADLTRQKIEALGRRALSFKVSITDRVGLKKLTRELVELWGGIDILVNNAAINRGDNFA
                     TTTDRAWDEVVNTNVHSLFAVTKPVYKQMIRQRKGAILNITSIGAIRALPTAVHYATS
                     KAAMIGFTKCLSREAATFGVRVNAIAAGIFETELAEALPERLLAMHDFWAAAGRKGRP
                     EELAEMAAFLVSERNSFMNGEVVIVDGGAVT"
     gene            12275..13462
                     /locus_tag="VF240_12375"
     CDS             12275..13462
                     /locus_tag="VF240_12375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7176048.1"
                     /translation="MPESSLTRHVKNLPYLKQIFREVERLRLELDQAVAGKQALANEK
                     SILEAERGALIAGRDSLAAAVRVLEDAVRVLGDELRSVGVERDALGVRATSLASENSS
                     LSAEKSALEAEVSRLRTWTAPGHFYSPFPAPEEILLKEHEIWPKPLPRKEIPGVDMNE
                     EGQLRLFREFLGYYREQPFTPRKQDRLRYYFENENYSYSDAIFLYCMLRHLRPRRYIE
                     VGSGFSSCVTLDTDELFLGSTLSCTFIDPQPELLLSLIKEEDKPRIEINGRKVQDVDI
                     HLFSELGEGDVLFIDSTHVVKALSDVNHIFFNVLPRLQRGVLIHIHDVFYPFVYPKDW
                     ELEKRGWNEAYVLRAFLQYNSAFEVYFCNTFLEYFYDAEFKREMPLCMKNTGGSIWLR
                     KIR"
     gene            13626..14087
                     /locus_tag="VF240_12380"
     CDS             13626..14087
                     /locus_tag="VF240_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7176049.1"
                     /translation="MATTELIDSLNRALSLELAGVIQYMQHSFLVTGTEREVFNEYFR
                     DQSKEAHGHARMLGDKVVALGGVPTVEPGEIRQAVELEAMLRQDLELERAAMQAYMEA
                     WKACGDEHMPTRFQLEERIADEQKHIEELEKLTSERKASVTRERITLRQIG"
     gene            complement(14168..14461)
                     /locus_tag="VF240_12385"
     CDS             complement(14168..14461)
                     /locus_tag="VF240_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176050.1"
                     /translation="MPVTNNAPEQVLGAAREEQRVEVVFEGSADAERVALKYSTWVEG
                     LGWCVQKTIRVEGDQLDELQRALMVARHRIRRRRAESGEIARPAQVIQMPALS"
     gene            complement(14652..15692)
                     /gene="lpxD"
                     /locus_tag="VF240_12390"
     CDS             complement(14652..15692)
                     /gene="lpxD"
                     /locus_tag="VF240_12390"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7176051.1"
                     /translation="MKLAELAELTNARLEGEVFPEVEITDAAGLDDAETGHVTFLSNP
                     RYTPRVQETRASAIYVAESVEVGRTDVAVLRARDPYLAYTRALRLFHPEPESESFIHP
                     SASIDPTARIGANVYIGACAVIGRDVEVGNGTRIYPNVTVYDGVRVGPGCTLHSGVAL
                     REGTRLGARVNVHNNAVVGCDGFGYAKDEEGRWLKIPQTGRVVVEDDVEIGAGTTIDR
                     ASVGETRIARGAKLDNLVQIGHSCTVGEDALLCAQVGLAGSSHVGRRVILAGQVGVAG
                     HLTVGDDAVLTAKSATSHNVPPGKMLSGIPAFDNREWLRSTAAFRRLGDMQRALRTLA
                     ARLTDLEKKSGG"
     gene            15878..18424
                     /locus_tag="VF240_12395"
     CDS             15878..18424
                     /locus_tag="VF240_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7176052.1"
                     /translation="MRPRAVDSFARHFPNPFRRAAHRLMLSPPALLSAAFALVLIACV
                     AAPGQTAYRKPPQPVLDVLNAPVPPSATISPARDQMLLATGVRYPPITELAQPMLRLA
                     GLRINPNLSAPHMSPYYVALSVKRISDGAETKIQLPAGAQIGFPQWSPDGRLFVFTNT
                     TPAGLELWVGETATAKVRRLKGVTVNAAYGQPVRWMPDGRTLLVQLVNAKRGASPVAA
                     SVPVGPTEQESSGKASGAATFQDLLRNAHDENLFDYYATSQLALIDSASGKITNLGQP
                     AIIDEAAPAPDGKHLLVAKVHRPYSYLHPSFYFPVDMEVWDTGGRVVHKLASLPLADQ
                     VPLQGVRTGPRSIQWRPTDPATLVWAEALDGGDPKKKVTHRDRVMSVKAPFTMSPNEI
                     FKTEHRYSGIQWGERGGLAFVTDFDRDRRWTRTQLINFDEAQAKPREIWSRSVNDQYG
                     NPGTPVTRVLPSGHRVVLHHNDSIYLTGAGASSDGDRPFLDRMSLQTLKTERLFRSAS
                     DAYETFVALLSDDARQIVTRRERPIEPLNYYIRTLGSGDASAGGSTDSAAAATRPLTQ
                     FPDPTPQLQGIKKQLVKYKRADGVDLSFTLYLPPGYKEGTRLPTVVWAYPLEFNDPAT
                     AGQVTGSTQRHTTIGGYSHLFFLLEGYAVLDNTTMPVVGDPEKVNDTYVEQIVASAKA
                     AIDKAVEMGVTDRERVGVGGHSYGAFMTANLLAHSDLFRAGIARSGAYNRTLTPFGFQ
                     SERRTFWEAPELYMKVSPFVVAHKINEPILLTHGEADNNSGTYPIQSERLYQAIRGTG
                     GTVRYVTLPLESHGYVARESTEHVLHEMISWFNKHVKNAGPRKPAEVTKK"
     gene            complement(18512..19039)
                     /locus_tag="VF240_12400"
     CDS             complement(18512..19039)
                     /locus_tag="VF240_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX7176053.1"
                     /translation="MRLRDLLPHFKRATHVGGVESDAHSAEPLSPHLALGQRGETLAA
                     EHLLHSGYRLAAANFKLPVGRNLRGSVVQAEIDIVAYEGHVLCFVEVKTRASDWFAAP
                     EANVDLRKQRQISRAARAYRRALGLAGEPYRYDVVSVVMPPPDEKGIAPAPRVELLRN
                     FWTDAKFKKRRWQER"
     gene            19297..20727
                     /gene="der"
                     /locus_tag="VF240_12405"
     CDS             19297..20727
                     /gene="der"
                     /locus_tag="VF240_12405"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7176054.1"
                     /translation="MSTNEQEVSEGSASEVRAPAAPLPLVAVVGRPNVGKSTLFNRLV
                     GERRSIVGDEPGITRDRIYGEAEWSGRRFSIVDTGGIVPDDEALIPSNILRQAEFAIQ
                     EAFALVWVVDVRSGVTQLDEELARLLRSTGKTVFVAANKTDTAKLEAHAAEFHQFGFE
                     DVIPISAEHGDGVGELLDILIAHLPRKDKAAEDESETEAREIRLAIVGRPNVGKSSLL
                     NRLLGEERVIVSPVPGTTRDAVDTVVEREGRSFRLIDTAGIRRKGKTEEMTEKLSVVM
                     ARRSLERADVAVVVVDAVEGVTALDANIAGYALDAGCSILIAVNKWDAVPDKLTGTPA
                     EFERDLRQKMKFLEWAPVVTISALSGQRVDKLLALAVRADEARATRISTSQLNDFFAR
                     AIAAPHAPTAKAQGGQTRIHVQYVTQVAVRPPRFVVFTAGGRPGLHFSYERYLHNQLR
                     EEFGFFATPLRIIERHKKKQRSEGRG"
     gene            20797..23550
                     /locus_tag="VF240_12410"
     CDS             20797..23550
                     /locus_tag="VF240_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7176055.1"
                     /translation="MPEESHLRTLFEHISDPAVVVGGGELFAAGGEVLAANASFAALA
                     GRPESELCGVEVSALLAFEGGASGGSSFGNLFAGDGEGWVPVKVERVPCRWEGREACV
                     LLVRPTTAARDREHETLRPEEVGALFDFLQEATEHVEVVNRVVAAVNSSRTIDEVFKL
                     ASQQIGAVVPFDRASIALCEEDCKRLRVFALSGEHAAPLQVGATAPLRGSVTEQALGE
                     RRALVIPDLAAETRFNVYDDLQREGFRSAVCVPLFSMERAVGSLNLTSRTPAAYGRRH
                     LLALERLAAPLAIAIEKVLLLEQSERRAREMGDRAEELSGLFEISRTFSTLTDTTEIS
                     ARLARAIAQLVGGETCLIATYDRKQNAVRAEAGHNTPPELLREFHFALERENTSAPVY
                     LTGEAFLSNDPGRDARLNHSFVERYRINSVLSVPMRIKRELIGFIYVGNRAGGFRERD
                     MRLLEIFSVQAAETIVNARLFLTIQAQAEREAVINRLLLSLQRGGEPRDKIGAVIERI
                     GQVLDLDRCIVVLFSEGEHDDYYGEWCAEGVAPVTHMADVRERSPVRYALRTARRPLI
                     ANDVTSHPLAAGNSELVERTQLKSMMVVPIMHLGRVIGSLSAHQTRARREWSEDDIDL
                     LTAVATHVGATLENARLITELREANRLKDEFLATLSHELRTPLTAITGWVDLLSESES
                     ARTDRDLADGVAAISASATSLTQLISDLLDLSRIQRGVLRLEREPLDVNQSVTSAARA
                     VRQGAAARRQELHLELAESLPHTIADAHRVQQILWNLLTNAIKFTPQGGRVTVRSRLL
                     DEGLKLGEQGAAGEHLRWIVIEVEDTGEGIPEEFLPFIWDRFRQADGSATRRHGGLGI
                     GLALVKELIEAHGGSAEAESRVGGGTSFSVRLPVIEVEEWMEREPRFQSPRGDGAGRG
                     EFS"
BASE COUNT         4153 a         7799 c         7656 g         3979 t
ORIGIN      
        1 gcggagacaa cggcggggcc gttcgacgtg gcggtcacgc ccctgagcga cggaaggcgg
       61 ctcgtcgccg cgcgctggcg cgaagagagc agcagtttgc gggaactcgc cgccggcgcg
      121 cagaccttcg aggacttcaa aaccaatcaa cgcaaggtgc gtctgctcgg tctctcgacc
      181 atcgggttga tgacgctgct gctgctcttc gccgccacct ggagcgccat ccatctcgcg
      241 cggggcttgg gcacacccat ccgcgccctc gccgaagcct cgaagcgcgt cgccgagggc
      301 gacctctcgc accgcgtcac cgccatcgcc gacgacgagc tggctgtgct cgccgccaac
      361 ttcaacgaca tgacggcgca actcgacgag aaccgccgcc gtctggagct caacgcctcg
      421 gagctgcgcg aaaccaacgc cgcgctcgac gagcggcgcg cctacattga gaccgtactt
      481 gagtcgctct cgacgggcgt cgtctccctc gacgcgcaga acaacgtgac gaccatcaac
      541 gccgcggccg cgcagatgct cggcctcgat tcgtcgccgc aacggggcgc gccgctcgcc
      601 gaagttgtga gcatcgacga ctacgagatg ctgagccgcg tgctgtggcg cgctcgcctg
      661 cgcggacgcg cctccgagca gacggagctt gcgcgcgggc ggagcgccga gggcaacggc
      721 gggcaagtgc cggagggcaa cggcggacag agtgaggaag gtgacaacgg caacaacacc
      781 cgccccgtcg cgctcacggc taccgccttg gggccgcgcg tggacgacaa ccgccgcggc
      841 gtcgtcctcg tcatggagga tttgacggag ctgctcgccg cgcaacgcgc cgccgcctgg
      901 agcgaggtgg cccgccgcat ggcccacgag atcaagaacc ctctgacgcc catccaactc
      961 tcggctgagc gcatcgcgcg caacttccag cgcctctccg atgcggaagc aacgaacggg
     1021 aacggcggag cgtccgcagc ggcgtccggc acgcgcagta gtgacagtga cgaggcgtca
     1081 gcagttttct catcgaacgg caacgaaggc ggacgcggcg ctcgggaacg cgagcgggtc
     1141 gcgggcatca tcgaggaatg cacggcgacc atcggccgcg aggtggcggg gctgaaggcg
     1201 atggtcgatg agttcgcgcg cttcgcacgg ctcccgcacg cgcgtctcgc ggcgggcgac
     1261 ctcaacgagg tcgtgcgcca atcggtcgcg ctctacgaag accgcctcgg cgacgcgcgt
     1321 ctcgacgtgc tgctcgcccc acgtctgccc gccgccctgc tcgacgccga gcagttgcgc
     1381 cgcgtcttcg tcaacctcat cgacaacgcc ctcgaagccc tcgaaggcgt gggcgacgag
     1441 cagcgcgtca ccgtcgccac ggcgcacgac ccggcgcgcg gcctcctcct cgtcgagatc
     1501 gccgacaccg gacagggcat cgcccgcgcc gatttcccgc gtctcttcca accctacttc
     1561 tccacccgcg ggcgcggcac gggcctcggc ctcgccatcg tccagcgcat cctcgccgac
     1621 cacggcggac gcgtccgcgc cgagtcgaac cgcccgcgcg gcgcgcgcat ggtcgtcgag
     1681 ctgccggtgg cggaagaagt aaaagaagag gtcagaggtt agaggttaga ggctagtgaa
     1741 gacaaaatgc tttttactga cctctaacct ctaacctctg acctcttgaa ttgctatgtc
     1801 tgactccatt ctcatcgttg acgacgagcg cggcatccgc gagacgctgc ggggcgtctt
     1861 ggaagacgag ggcttcgcgg tggaggcggt cggctcgggc gaggagtgtt tgaaggccgt
     1921 ggagcggcgc gcctacgggt gcgtgcttct ggacgtgtgg ctcccggaga tcgatgggct
     1981 ggagacgttg cggcgaatgc gcgagcaggg gacggacgcg gccgtggtga tgatctcggg
     2041 ccacggcaac atcgagacgg ccgtgcgcgc caccaagctc ggcgccttcg acttcatcga
     2101 gaagccgctc tccatcgaga agaccgtgct caccgtcaga aacgccttgc ggcagcgcgg
     2161 gctggagcgc gccgtggccg cttacgccga cgggatgcgc ggcgcaaacg agatggtcgg
     2221 cgagtcggtc gcgatgcgcg cgctgcgaaa gcagatcgag atggtcggcc ccacggacgg
     2281 ccgcgtcctc atctacggcg agtcgggcac gggcaaggaa ttggtcgcgc acgccatcca
     2341 cgccacgtcg cgccgcgcgg gcgcgccctt cgtcgaggtc aactgcgcgg ccatccccga
     2401 ggagctgatt gagtcggagt tgttcggcca cgtccgcggc gcgttcacgg gcgcgaccgg
     2461 ggccaagcgc ggcaagttcg agatggccga cggcgccacg ctttttttag acgagatcgg
     2521 cgacatgagc gcgcgcgtgc aggcgaaggt tttgcgcgtg ctcgaagagc agcggttcga
     2581 gccggtgggg agcacgacct cgatgcgcgt ggacgtgcgc gtgctcgcgg cgacgaacaa
     2641 gcgtctcgaa gaggagatcg agcgcggggc cttccgccct gacctcttct accgcctcaa
     2701 cgtcatcccc ttcgagctgc cgcccctgcg cgagcgactc gaagacatcc ccgccttagt
     2761 cgagcacttc aacaaaaaat tctccgccgc ctacaaccga cggcccaaac gcttcgagcc
     2821 ggaggcggtc gaggccatgc ggggctactc ttggccgggc aacgtgcgcg agctgcggaa
     2881 cacggtcgag cgcgtcgtca tcatgcaccc gcacgtcaaa gtctcggccg ccgacctgcc
     2941 cgcgcgcggc ggcgaggccc cgcccgccga ccccctgcgc tacgccaact tccgcgaggc
     3001 cagcgaagcc ctccaacgcg agttcatcaa gcgcaaactc gcggaggccg agggcaatgt
     3061 ctcacgcgcc gccgaactcc tcggcatgga tcgctcacac ctctaccggc gcatgcgcgc
     3121 cctcggcatc aacgtccgcg aggaacgcgc cggcgccgtc taaccgcccc ggccggcgcg
     3181 gcctactccc ggccgctttg cgatctgatt tcctcgtcgg tggctgcgcg ccacagcctc
     3241 acgtccccgt cagcttcccc cgcggcgagc gtcctcccgt cgggcgagaa ggcggacgcg
     3301 gtcgcgtagc tgctctcgtc ctgcaccgag accatttccc tgaacgagat cgcgtcccac
     3361 agtttcgaca tcccgcccgc gccgctgctg tagagcgtct tcccgtcggg cgagaaggcg
     3421 agcgacgtga tggtgtctct gtgccctctg agccgcacca actctctggg tgcgcgtagg
     3481 ccctcgccgc ccgtgacttc ccacagcctg agcattcccc cgccgaaatg gaccctcggg
     3541 tcagcatccg agaaatcttt catcaggccg acggcgagca gacggccgtc gggtgagaag
     3601 gcgagcgtgt ttggcggggg caggactcca tcactctcaa gttccgtcaa gacattcaag
     3661 tctctatccc aaagtttgac cgcgtcctcc ccatgaaaga tggccgcgat ggcctgacca
     3721 tctggtgaga aggcgacggt cttgacggtg ttcggcgcgc cgctcacttc tcgcaatttc
     3781 gtttgtgtgg ccggttccca gagcgagatg tagccctgaa tgtcgccggt gactaacagc
     3841 ttgccgtccg gcgaagagga caaggcggtg acgcggcgct catgcggtag cgcccacact
     3901 tccttgcgcg tgacgctgtc gagcaggtga accctccctc cggcgtcact gactgcaagc
     3961 gtcctgccgt caggcgagaa gtggaaaaga tcaacgtcgg agaccgcacc caggtcttcc
     4021 aaccggcggc gcgtggcgac atcccagagc gcgacggtgg agtccagatg gacggaggcc
     4081 agcatcttgc cgtcgggcga gaaggagacg gatttaacat tccgacccgg cccctcgccg
     4141 gggtcttcgc gcacctcgcc gctccccagc ggcaactgtg cgcgcaccgc cgacgacacg
     4201 tcccagaccc tgaccgcatc cttgctgccg gtgaagaggg tctggccgtc gcgggaaaac
     4261 gccagcgagc gaaccgcctc ctgatggccg ccgagcgtga ccccgtgcgc caggaagacc
     4321 gaatcgtgaa ccttgacact gccgttcttg tagccgatgg cgagcgtctt tccgtcgggc
     4381 gagaatttga gcgacgtgat gtcattcgtg gcgggaattt tatcccccca ggcgttttgt
     4441 gagaggtgcc attgccacac cactctgtcg gagaatccga tcatcagcgt cttgccgtcg
     4501 ggcgcgaagg agatggcttt aacgtcgtcg gtgatcggcg tgatcttgca cgggggaggc
     4561 gcagtctcgc actcagaaat cttctcatac ctgaccggac tccgaagctt gccgaggtcc
     4621 cagaggatga tgggcgaggg agccttgccg ccggtggcga gcatcttgcc gtcgggcgag
     4681 aaggccagcg cgtcggggtg gtttgcgccc ttaatgaatt ccgtctctcg gagcaggtta
     4741 acgtcccaca gcttgacgtg cccgtcgatg ggggtcgtcc aggctaacaa cttgctgtcg
     4801 ggcgagaagg cgaaaccggc cgggcgatcc tcgatctccg cgaccgtgcc cttaagttcg
     4861 cccgtggtgg tgtcgagcaa aatgtatttc ggtttgctgg agtcgggggg agtgccgatg
     4921 gcgagaagcg ttttcccgtc gggcgaaagc tccacggcgg tgagccggtg gtcatcgagg
     4981 ccgcggaagg gggccgggcg ggcggcgccg gtggccgggt cgcgcagttc gatttggttg
     5041 ttggcgccga cgatgagcgt ctggtcgtca gacgcgaggg ccatgaagtt cgagccgtct
     5101 ggaaaattga tcatggcctt ctcgctgttg atgacgcccc agaggtagcg ccactcgaac
     5161 ctgagcgcgc cgtcgtcggc cgcgcgatgc gaatcaagta gctcgcccgc gtgctggagg
     5221 ttgttcgcca cgaaagcctg ctgcgcgagg tggatgtcgg ccgggtagag gagctggcgg
     5281 ttctgcgtcg ccagctcaag ggcgcgcgcc tcctgcgcgc tggccagcca cttctggcgg
     5341 aaggcgtaga cggtggagcc gagcgcgagg acgacggaga aggcgaggag ggcggcgacg
     5401 agcttggtgc gtttgaagga gacgtcgcgg cgctgctggc gctcgcgctc gcgcgcctcg
     5461 gcgtcgcggg cggcgacgct tgagtcgagg aagctggcgg ccgtctcgaa ctcggggtgg
     5521 tagcggcgcg cccacgcggc attcgggcgg gtgcgctcgc gccagtcgag ggcgacttgc
     5581 agcgcggggt ctttcaagag cgcctcctcg cccgccttgt gaagcacggc cgtctcggcg
     5641 aggcggcgat agatgcgcgc cgactgcgcc tcctcgtccg tccaattctt cagacgcgcc
     5701 cagttgcgaa tcagactctc gtgcgagatg tcgatgagcg agtcggcgtc taagggcgcg
     5761 ggcggcggcg gcatcagaaa cgagcggccc ggccggcgga aggtttcgac gacggcgacg
     5821 acctccgctt cgtcggcctc gacgagcgcg caggcgtcgc ccaattccag cgggcggcgc
     5881 gtctcgcggt tgtccgcgcc cttctcagtg agcgccttga agagtcgctc ggcgattttt
     5941 tgtcgcggct cggagagttc gccgaaggct tcctcggcgt ggagcgacag agcttctttc
     6001 atgccgccga tggcctgata gtggggcagg tcgattggct cgccgtcgcg gcgcaccgag
     6061 agccagtgat cccacgtccg catgagcgcg tgctggagga taggcaactg gtcggggttg
     6121 tcgcccatgt cgttgaggag ctggttgacg agagggacgc tgatgcggcc gtcgcccacg
     6181 gagacggggc cggtgacggc ctcgcgccgc tcgtctctgg tcatgcgcgg aatgagatac
     6241 tgcccgtcgt taatcgcctc gggcaatcct tgaaactgcg cgcagtcgcc gaggaagtcc
     6301 gagcgcatcg tcagcacgac gtaaatccgc tcgcccggaa cgccgacggc ttcgagcagc
     6361 aggcgcacga agccggccgc gtcctccttc gtgttctcga cctccatcag cgcgcggaag
     6421 cgaaacagct cctcaaactg atcgacgacg acgagcaggt tctccgaggc cgcgaggcgc
     6481 ggacgaccgc cttcgccgcc cgccgcggcg cgcgcttgcc tgacggcgtc gagcaggccg
     6541 aggctgctgc gccggagcgt cgcctcgacg agcgccgtct gcatctcggc ctctctgggg
     6601 tcgggcgcgc cgaagacttc gggcgaggcg agcgtgcggg cgaggttgcc gagggggtcg
     6661 tggccggggc gcagcatggc gacgcgccac tcggagccgg cgctggccat cagtccgccg
     6721 tggagtgcgg ggagcagacc ggcgcgcacg agcgacgact tgccgctgcc cgatgtgccg
     6781 acgacggcga ggaaacgcgt ctgcccgaga cggcggatga gttcttcgct ctgcccgtcg
     6841 cgcccgaaga agaggtgcgt ctcgtccgac tcgaagggcc ggaggccggg gaagggattg
     6901 ggcatcgtcg tcgtcattgg tcgtctccgt ccgtcgcggt tcgtcccagc gagtcgagga
     6961 acggcttcag gctctcggaa gtaaagtcgc cgaagttctt cacgaagaaa gaatcgcgcg
     7021 tgcggaagag ttgtttgtgt ggagacacgg ggctgccgac gtagacgccc cacgcgagcg
     7081 gcgttcgtcc ggcgtcgcgc ccctgcgcgg cggccttctc cagaccgcgc cgctggtagt
     7141 cgaaccaagc ctgattggcg ctgccgtagt agacgagcgc ggcgtcgcag gtggcgaggt
     7201 tctccttgtg tgtctcgatg gcctgcgcgt cgtcgcccgc gcccacgtcg aggagcggga
     7261 ggagcacgtc gtagccgcgc tcgtagagga agtcgttgag ggcgacgatg ccttccacgt
     7321 cgcgcgggtc gcagaggaga tagattttct tctgggcctg cgccccgtcg ccggcgtccg
     7381 ccgccgcgcg gtggccgttg ccgctcagcc tgacctgaat gacggacttg agttcttcga
     7441 gcgtggtctg caaaagctcc gcgccccgct gcgggtcggc gctcgtcttc agatactcga
     7501 tgaactcggt ctgcgtcccc tcctgcgggg ccagacgctc gggcagccag atgagtcgct
     7561 tgaaggcggg gtcgccgctg cgctcggcgg cgatctcgtt ctggagtcgg acgacggagc
     7621 tgcccgaagc gccttcgggg atgatgccgt agctggcccc gatgaggtgg actgaaagct
     7681 tggcgcgcag cacaccctcg cgcacggcct cctgatactc gggcgacttg tagggcagct
     7741 cgcggttggg caggatgtcg tagccgcgct cctgcaactc gcgcttgatg cggtcgcgct
     7801 cctcgcgcaa atcatagctc gtctcggcca gatagacggt gcccttagag gcgccgaaga
     7861 cgtggggcac gaccgccgcc gtcgtcgttg gcggcgacgc ggcgacgagc gtctgcttga
     7921 tgtcccaggc gaggtcgttg agcttgtccc agtagcgttg gtcgcggttg cggcccgtct
     7981 gttgcccgta ctcgacgggg cgacccgtgg cctgttcgat ctcgtagaac tcgtaaccga
     8041 gttgtccctg aaactcggcc gggtgctggt cgcgctcaac gaaggtcttg acgaccttga
     8101 agacgcgcag ccggccgccg cccaccttca ggccgccgtt ctcctcagcc gcccggcaaa
     8161 actccaccaa ctcgcccatg caccacttcg actggagata gcccggcgag agcacggaga
     8221 ctaagacgca cgtggtgcgg agtcggtcgg tgagttcgtt ggcgaaatag acgttgccgg
     8281 gcaggcgcgg gtcgcgccag atgcgcgggc ggtggccgag gagttcgccg aggcggtatt
     8341 cgagtctttc gtgcagatta tcgacccacc cgcgctcctc gtcttctaag ggctggttgt
     8401 cgatgtggcc gtagctgatg agggcgtcgt ctgcgtagcg ggggcctgtg ctcattaatc
     8461 tctccacgga cggacactgt ggcggctgcg cgggcgtgcg gaatgtggaa taacgaaaac
     8521 cccgagggcg cggaagaccc cgtgggcgcg ccatgaggaa cggacaacgc actaaccaaa
     8581 accctcggcg cgagccgatt ttagtacggg cgagggaatt gtcaaaacat ctcgcccacg
     8641 gctcgcgtcc ggtcgcggac gcatcgcgtt gacagccccc gcgaaatgcc gtatctctaa
     8701 atcttattcg tctcacgtga gcgaataaca tgacaacgcg gggcgcgtcc ccggcgtcta
     8761 aagcagggtt aataatggac tacacgacca gagaattact taaacgctac gccaagctcg
     8821 cgctgggcca acctttcgcg cccgtgctcc tgaacatcct cgtcaccagc gtctgcgaca
     8881 tgcgctgcac gcattgtttc ttcacggacg aactcgacga ccggccccgc aagaagctcc
     8941 agatgaagac gcacgagatt caaaaaatct ccgagacgct gggcggcaac ctcggcgtcc
     9001 tcatcctcgc gggcggcgag ccgttcacgc gcaaggattt gcccgagatc gtccgcgcct
     9061 tctacgagaa caaccggctc gaatccgtct acctgatgtc gaacgggcag atacagaagc
     9121 gtatcatccc cgacgtgacg cgcatcctcg aagagtgccc gaacttaaac gtcgccgtcg
     9181 ccctcggcat cgacggcctc aaagatcagc acgacaagat tcgccagaag cccggctcgt
     9241 gggacatcgc catcgacacg gcccgccagc ttcaggccat caagcgcgag cacccgcgtc
     9301 tcgacatcca gacctgcacc tgcttcatga actcgaatca ggacaccatc ttcgactggt
     9361 acgaatatct caagcacgac ctgcgccccg acaaggtcaa cttcaactat atccgcccgc
     9421 cctcggctga ccccgtggaa ctcgacatcg accacgcgcg ctacgcgaag ctggcgcaca
     9481 tgatcgacga cgactcgcgt cacgccacca tcaagaacaa ctacggcggc gacgccggct
     9541 tcttcaaggc cgccatcgac atctacatgc acggcctcat tgccaagacg caggaggagc
     9601 agaaggcgca gatgacctgc tacgcgggca cggccggcgc tgtcatctac gacgagggca
     9661 ccatctcttc ctgtgagaac aagacgccgg tcggcaacct ccgcgaccac gactggaact
     9721 tccaatcgct ctggcgctcc gaggccatga aggcgcgacg caaggaggcg gccgacggct
     9781 gcttctgcac gcacgagtcg aacagctact acccgagcct gcccttcaac ccgaagcacc
     9841 tgattcaaat caagcgactg gagcgcgaga tgaagaaggc gagcagggaa ctcgaacgca
     9901 agcagggcga gggcgagggt ctggccatca aggcttaaaa gctgccctct caatcgaaca
     9961 tgtttagagc aagggtcccg aaaatcctga tcatctcgtc cgacacgggc ggcggccacc
    10021 gctcggcggc gacggccatc gtcgccgggg tgcagaagtt tctcaagagc gactcttacg
    10081 ccgtccgcgt cgtgcgcgcc ctcgaagagt cgcaccacct gtcggcgaag atggtcggtg
    10141 tttacaactg ggtgctgcga caccgtcagc actggatgaa atatctctac tgggccatca
    10201 accgctttcg cccagagacg cgcgagttct tctaccgccg ctacgtcggc tacgtccgcg
    10261 aactcttcga ccgttggtgc ccgcacgtcg tcgtcagcgt tcatcccctg acgcagcacg
    10321 ccttcgcgcg tgtgctcaag gagcttgggt tagccggcac gatcccactc gtcaccgtcg
    10381 tcaccgaccc ctgctacggc ttctggaagg gctgggcctg cgatgacgtg acgctctacc
    10441 tcgtcgccag cgacgatgcg cgccggcagc tcatcgatta cggcatctcc cccgagcgca
    10501 tcaaggtctc ggggatgccc gtccacccga agttcgattt gccggacgag cgcgccgcgc
    10561 aggccgcgcg caccgcgctc ggtctcgacc cggagaagtt caccgtcttc gtcaacgccg
    10621 gctgggtcgg cggcggcaac atccccgaaa tcttccgaga gcttgtgcgc ggcgacctcg
    10681 acgtgcaggc cgtcttcctc gccggccgta acgaagagtt gcgcgccgag gccgaagggc
    10741 tggccgccgg cgcgaagttc cccgtcaagg tcatcggcta ctccgaacac gtcgagcaga
    10801 tcatgcaggc cgccaacgtg atggtctcga agctcggggg cttaacgacc ttcgaggcgc
    10861 tcgcctgtcg cgtgcccatc atcgccgacg ccatcacgaa gccgatgccg caggagtcgg
    10921 gcgcggccac gctcatctcc acgcgcggcg cgggcgtgct cctcaagcgt gcaaatgaca
    10981 tcgtgccggt ggtgcgccgc atggttgacg acgcgctcta ttacgccgcc atgcgtaagg
    11041 ccgcggcctc cgtcgccgta cccgacgcca cgcgccgtat cgtcgaggaa atcgccgcgc
    11101 tcatccccgc ccccgtgatg gcgaaggagg agccggcggc cgctgaaatc cgcttggctt
    11161 aacaagggac gacgcgcgcc ggttgactcc cgcccgccgt taaggcttta atccacacac
    11221 gctcgaaaat cagcggcccg cttcgccccg acggttgagc gggcttttta aggggtgaga
    11281 cttttgagtc ttctgctgga aggcaagcgc gccttcatca ctggcggctc gcgcggcatc
    11341 ggcgccgcgc tctgcgaggt cttcgcgcgc gagggcgccg acgtggcctt caactatcac
    11401 tcgcgcgacg agctggccga cctgacgcgc cagaagatcg aagccttggg ccgccgcgcg
    11461 ctctccttca aggtctccat caccgaccgc gtggggctga agaagctgac gcgcgagctg
    11521 gtcgagctgt ggggcggcat cgacatcctc gtcaacaacg ccgccatcaa ccgcggcgac
    11581 aacttcgcca ccaccaccga ccgcgcctgg gacgaggtcg tcaacacgaa cgtccacagc
    11641 ctcttcgccg tcaccaagcc cgtctacaag cagatgatcc gccagcgcaa gggggcgatc
    11701 ctcaacatca cctccatcgg cgccatccgc gccctcccca cggccgtcca ctacgccacc
    11761 tcgaaggcgg ccatgatcgg cttcaccaag tgcctgtcgc gcgaggccgc taccttcggg
    11821 gtgcgcgtca acgccatcgc cgccggcatc ttcgagaccg aactcgccga ggcgctcccc
    11881 gaacggctcc tcgccatgca cgacttctgg gccgccgccg gacgcaaggg ccgccccgaa
    11941 gaactcgccg aaatggccgc cttcctcgtc agcgagcgca acagctttat gaacggcgag
    12001 gtcgtcatcg ttgacggcgg ggcggtaacg taaaagctgt cagctatcag ctatcggctg
    12061 aaaccgtccg tggtcagttg tccgtggttc acgtccgtct tgcttcgctc gcacatccgc
    12121 aacgctcagc acaaaatcac ttctaccgcc ttcctttatt ttcgctcgct tgcttctccg
    12181 ggttaatata ttcttcaacc tacgtgttta cttagatgag agaattagcg gcactggtca
    12241 tgagtacgta agcctctaaa cctagtgtga agagatgccc gaaagctcgc tcacaaggca
    12301 cgtcaaaaac ctgccgtacc tcaaacaaat cttccgagaa gtagaacgtc tcaggctcga
    12361 actggatcag gccgtcgccg gcaaacaggc gttggcgaac gagaaatcaa tcctggaggc
    12421 ggaaaggggc gcgctcatcg ccggtaggga ttcgctcgcg gcggccgtcc gggtgctgga
    12481 ggatgcggtc agggtgttgg gggacgagtt gcgctccgtt ggcgtggaga gagatgcgct
    12541 cggcgtgagg gccacctcac tcgcatcgga gaacagttcg ttgagcgcgg agaagagcgc
    12601 gctggaggcg gaggtgtcgc gcctcaggac gtggacggcg cccggacact tctactcccc
    12661 tttccccgcc cccgaagaga tactgctcaa ggaacacgag atttggccga agcccctccc
    12721 gcgcaaagaa atccccggcg ttgatatgaa cgaggagggg cagttgcgcc tcttccgtga
    12781 gttcctcggc tactaccgcg agcagccgtt cacgccccgg aagcaggatc gcctgaggta
    12841 ctacttcgag aacgagaact attcctattc cgacgccatc ttcctctact gtatgctcag
    12901 gcacctgcgc ccccgcaggt acatcgaggt cggctccggc ttctcctcct gcgtcaccct
    12961 cgacacggac gagctattcc tgggcagtac cctgagctgc accttcatcg acccgcagcc
    13021 cgagttgctg ctctcgctga ttaaagagga agacaagccc agaattgaga tcaacgggag
    13081 gaaggttcag gacgtggaca tccacctgtt ctcggagttg ggtgagggcg acgtgctctt
    13141 catcgactcg acgcacgtcg tgaaggccct gagcgacgtg aaccacatct tcttcaacgt
    13201 cctgccgcgt ctccagagag gggtgctgat tcacatccac gacgtcttct accccttcgt
    13261 ctacccgaag gattgggaac tggagaagag agggtggaac gaggcctacg tcctgcgcgc
    13321 cttcctgcaa tacaacagcg ccttcgaggt ctacttctgc aacaccttcc tcgaatactt
    13381 ctacgacgcg gagttcaaaa gggagatgcc gctgtgcatg aagaacaccg gggggagcat
    13441 ctggctgaga aagattcgtt aggacttccg ttgctattaa ctgacggcta acggctttaa
    13501 tgaaatcgac tttcattatt aagtcgtgca ttttcaacat ttacggttga cagtggagcg
    13561 gttcgagatt aatattcgca cagtttgaag gtattctttt ttgggttgaa tgggggacgg
    13621 tagttatggc gacgaccgag ttgatcgaca gtttgaatcg cgcgctgagt ctggagttgg
    13681 cgggcgtgat tcagtacatg cagcacagct ttctggtgac gggaacggag cgcgaggtct
    13741 tcaacgaata cttccgcgat cagagcaagg aggcgcacgg ccacgcgcgg atgctcggtg
    13801 acaaggtcgt ggccctcggc ggcgtgccca cggtcgagcc gggcgagatc cgtcaggctg
    13861 tcgagcttga ggccatgctg cgtcaggatt tggagcttga gcgcgcggcc atgcaggctt
    13921 acatggaagc gtggaaggct tgcggcgacg agcacatgcc cacccgcttc cagctcgaag
    13981 agcggatcgc ggatgagcag aagcacatcg aggagctgga gaagctgacc tcggagcgca
    14041 aggcgagcgt cacgcgcgag aggatcacgc tgcggcaaat cggctgacag agcggggaac
    14101 tgaaaaccga agaggcgcgg ggtagcacga cggctacctc gcgcctcttc ggtttgagta
    14161 tggacgatta agagagcgcc ggcatttgga tgacctgcgc gggacgggcg atttctcccg
    14221 actcagcgcg gcggcgacgg atgcgatggc gcgctaccat gagcgcgcgc tgaagctcgt
    14281 cgagttgatc gccttcaact ctgatcgtct tctgcacgca ccagccgagg ccctcgaccc
    14341 aggtcgaata cttgagggcg acgcgctcgg cgtcggcgct accctcgaaa acaacctcca
    14401 cccgctgctc ttcgcgcgcg gctccgagga cttgctccgg cgcattatta gtcactggca
    14461 cggcccgtat tgctccttaa gagatgatga gtgcttcgga aaacttcgcc ggacgtgggc
    14521 gaaattgaaa tccaatttca ttatccacgt gagaggtgaa ggtgtcaaat gaaaaatatt
    14581 ggtcggggaa gcaggtggac tctgccgcgt ggaaaccagt ggcagttgcc gcgtccgaag
    14641 ggtgttggag gttagccgcc ggattttttt tcgaggtcgg tgaggcgcgc ggcgagtgtg
    14701 cgaagggcgc gctgcatgtc gccgaggcgg cggaaggcgg cggtcgagcg tagccactcg
    14761 cggttgtcaa aggcggggat gccggagagc attttgccgg gggggacgtt gtggctcgtg
    14821 gcgctcttgg ccgtaagcac cgcgtcgtcg ccgacggtca ggtggccggc gacgccaacc
    14881 tgaccggcga ggatgacgcg ccggccgacg tgagagctac cggcgagtcc gacctgcgcg
    14941 cacagcagcg cgtcctcgcc gaccgtgcag gagtgtccga tctggacgag attgtcgagc
    15001 ttggcgccgc gggcgatgcg cgtctcgccg acggaggcgc ggtcgatggt cgtgcccgct
    15061 ccgatctcca cgtcgtcctc gacgacgacc cggcccgtct gcggaatctt cagccagcgc
    15121 ccctcctcgt ccttcgcgta gccgaagccg tcgcagccga cgacggcgtt gttgtgaacg
    15181 ttgacgcgtg cgccgagacg ggtgccctcg cgcaacgcaa cgccggagtg gagcgtgcag
    15241 ccggggccga cacgcacgcc gtcgtagacg gtcacgttcg gatagatacg cgtgccgttg
    15301 cctacttcaa catcgcgccc gatgacggcg cacgcgccga tgtagacgtt cgcgccgatg
    15361 cgcgcggtcg ggtcgatgga ggccgatggg tggatgaacg actccgactc cggctcggga
    15421 tggaagagac gcagggcgcg cgtgtatgcg aggtaagggt cgcgggcgcg caggacggcg
    15481 acatccgtcc ggccgacttc gacgctctcg gcgacgtaga tggctgaggc gcgcgtctcc
    15541 tgcacgcgcg gcgtgtagcg cgggttcgac aggaaggtga cgtgccctgt ctcggcgtcg
    15601 tcgaggccgg cggcgtcagt aatctccacc tccgggaaca cttcaccttc aaggcgcgcg
    15661 ttcgtcagct cggcaagttc tgcgagtttc ataagctcag tttatcgcac acagggagtt
    15721 caaacttcaa agtctgcgtg atatcagagg cgcgcccggc gggaaagttg actatctttc
    15781 gtcgttggga gaaacttccg ccgtcaaccg tcaataatta acgcaagtct ttcaacaaac
    15841 aattcgaccc gcttcgttcg ctgaggaggt aatttctatg cgccctcgtg ccgtcgattc
    15901 attcgcacgc cactttccca accccttccg ccgcgcggcg catcgcctga tgttgtcgcc
    15961 gccggcgctc ttgagcgcgg cgtttgcgct cgtcctgatc gcgtgcgtcg ccgcgccggg
    16021 gcagacggcc tacaggaagc cgccacagcc cgtcctcgac gtgctcaacg ccccggtccc
    16081 gccgagcgcc acgatcagcc cggcgcgcga tcagatgctg ctcgcgacgg gcgtgcgcta
    16141 tccgccgata accgaactgg cgcagccgat gctccgcctc gcgggcctgc gtatcaaccc
    16201 gaatctctcc gcgccgcaca tgtcgcccta ctacgtcgcg ctctcggtca agcgtatctc
    16261 cgacggcgcg gagacgaaga tacaactccc ggcgggcgcg caaatcggct tcccgcagtg
    16321 gagccccgat ggccgactct tcgtcttcac caacaccacg cccgcgggtc tggagctgtg
    16381 ggtgggcgag acggcgacgg cgaaggttcg acggctgaag ggcgtgacgg ttaacgccgc
    16441 ctacggccag cccgttcgct ggatgcccga cgggcggacg ctgctcgtgc agctcgtcaa
    16501 tgcaaagcgc ggagcgtcgc ccgtcgccgc gagcgtgccc gtggggccga ccgagcagga
    16561 gagttccggc aaggcttccg gcgccgccac gtttcaagac ctgcttagaa acgcgcacga
    16621 cgaaaacctg ttcgactact acgcgacctc acagctcgct ctcatcgaca gcgcgagcgg
    16681 taagataacc aacctcggac aaccggccat cattgacgaa gccgcgcccg ctcccgacgg
    16741 caagcacctg ctcgtcgcga aagtccatcg tccgtactcg tatctgcacc cgtccttcta
    16801 cttccccgtt gacatggagg tgtgggacac gggcgggcgc gtcgtccaca agctcgcgag
    16861 tctgcccctc gccgatcagg tgccgcttca gggcgtgcgg acggggccgc gctctatcca
    16921 gtggcgtccg accgacccgg ccacgctcgt ctgggccgag gcgctcgacg gcggcgaccc
    16981 gaagaagaaa gtcacgcacc gcgaccgcgt gatgtcggtg aaagcgccat tcactatgtc
    17041 gccgaacgaa atcttcaaga ccgagcatcg ctattccggc atccagtggg gcgagcgtgg
    17101 cggcctcgcc ttcgtcacag acttcgaccg cgaccgtcgc tggacgcgca cgcagctcat
    17161 taacttcgac gaagcgcagg cgaagccgcg cgagatttgg agccgtagcg tcaacgacca
    17221 gtatggcaac cccggcacgc ccgtcacgcg cgtcctgccg agcggccacc gcgtcgtcct
    17281 ccatcacaat gactcgatct atctcaccgg cgccggcgca tcctccgatg gcgaccgccc
    17341 gttcctcgac cgcatgagcc tccagacgct caagaccgag cgactcttcc gcagcgcgtc
    17401 ggacgcttat gagactttcg tcgcgcttct ctccgacgac gcgcgccaga tcgtcacgcg
    17461 ccgcgagcgc cccatcgaac cgctcaacta ctacatccgc acactcggca gcggcgacgc
    17521 atcggccggc ggctcaaccg acagtgcagc cgccgccacg cgtccgctca cacagttccc
    17581 cgacccgacg ccgcagcttc agggcatcaa gaagcaactc gtcaagtaca agcgcgccga
    17641 cggcgtcgat ctctccttca cgctctacct gccgcccggc tacaaggagg gcacgcgcct
    17701 ccccacggtc gtctgggctt acccgctgga gttcaacgac ccggccaccg ccggacaggt
    17761 caccggctcc acgcagcgac acacgaccat cggcggctac tcgcatctct tcttcctgct
    17821 cgaaggctac gccgtcttag acaacaccac gatgcccgtc gtcggcgacc ccgagaaggt
    17881 caacgacaca tacgtcgagc agattgtggc gagcgcgaag gcggccatcg acaaggccgt
    17941 tgagatgggc gtcaccgacc gcgagcgcgt cggcgtcggc ggccactcct acggcgcttt
    18001 tatgacggcc aacctcctcg cccactccga cctcttccgc gccggcatcg cccgcagcgg
    18061 cgcttacaac cgcaccctga ccccgttcgg tttccagagc gagcgccgca ccttctggga
    18121 ggcgcccgag ctttacatga aggtctcgcc cttcgtcgtg gcgcacaaga tcaacgagcc
    18181 gattttgctc acccacggcg aggccgacaa caactcgggc acctacccca tccaatcgga
    18241 acgtctctat caggccatcc gcggcaccgg cggcaccgtc cgctacgtca cgctcccact
    18301 cgaatcgcac ggttacgtcg cccgcgaatc gaccgagcat gtactccacg agatgatctc
    18361 ctggttcaac aagcacgtga agaacgcggg gccgcgcaag cccgcggagg tgacgaagaa
    18421 gtaaacagaa gtcaggagtc aggggtcaga agtcagaatg aagaaagcat ggctgctttt
    18481 attctgactt ctgactcctg actcctgact cctatctttc ctgccagcga cgctttttaa
    18541 acttcgcgtc cgtccaaaag ttgcgtagca gttcgacgcg cggggcgggc gcgattcctt
    18601 tttcgtcggg cgggggcatg acgacgctga ccacgtcgta gcggtaaggc tcgccggcga
    18661 gtccgagggc gcggcggtag gcgcgcgcgg cgcgggagat ttgccgctgc ttgcgaagat
    18721 cgacgttggc ctcgggcgcg gcgaaccaat ccgacgcgcg cgtcttcacc tcgacgaagc
    18781 agagcacgtg gccctcgtag gcgacgatgt cgatctcggc ctgcacgacc gagccgcgca
    18841 ggttgcgtcc cacgggcagc ttgaagttgg cggcggcgag tcgatagccg gagtgcagca
    18901 ggtgttctgc cgcgagcgtt tcgccgcgtt ggccgagcgc gaggtgcggc gagagtggtt
    18961 cggcggaatg ggcgtcgctt tcaacgccgc cgacgtgagt cgcccgctta aagtgaggaa
    19021 gcaggtcgcg taagcgcatg ccgcgcatta taactcacat tcaatggcgg agggatcatc
    19081 ggcacataca gtaccgcgag cggtagcctc gcgggtcggg ttgcatgggg cgggcctctc
    19141 gccgcagcca cacccgctcg ctaccgcgcg cggtactgtg cttgagtcaa ccggcgagtc
    19201 aatccgtctt catccttcat gccatcttgc ggcgcgcact cggcaggcga tacactcgcg
    19261 agtaccccgc gtccgattca atacgaaaat tatcttatga gcacgaacga gcaggaagta
    19321 agtgagggaa gtgcgagtga ggtgcgtgcg ccggcggcgc cgctgccgct cgtcgccgtc
    19381 gttggccgcc cgaacgtcgg caagtcaacg ctcttcaacc gcctcgtcgg cgagcgccgc
    19441 tccatcgtcg gcgacgagcc gggcatcacg cgcgatcgca tctacggcga ggcggagtgg
    19501 tcgggccggc gcttctccat cgtggacacg ggcggcatcg tccccgacga cgaggccctc
    19561 atcccctcga acattctccg tcaggcggag ttcgccattc aggaagcgtt cgcgctcgtc
    19621 tgggtcgtgg acgtgcgctc cggcgtcacg cagctcgacg aggagctggc gcggctcctt
    19681 cgctcgacgg gcaagaccgt gttcgtcgcg gcgaataaga ctgacaccgc caagctcgaa
    19741 gcgcacgccg ccgagtttca ccagttcggt ttcgaggacg tgataccgat ctccgcggag
    19801 cacggcgacg gcgtcggcga actgctcgac attctgatcg cgcacctgcc ccggaaggat
    19861 aaggccgcgg aagacgagag cgagaccgag gcgcgggaga tacgtctggc gattgtcggc
    19921 cgcccgaacg tcggcaagtc ttcgctcctc aaccgcctgc tcggcgagga gcgcgtcatc
    19981 gtctcgcccg tgccggggac gacgcgcgac gcggttgata cagtcgtcga gcgcgagggc
    20041 cgcagctttc gactcatcga cacggccggc atccgccgca agggcaagac cgaggagatg
    20101 accgaaaagc tctcggtcgt gatggcgcgc agaagccttg agcgcgccga cgtggccgtg
    20161 gtcgtcgtcg atgcggtgga gggcgtcacg gcgctcgacg cgaacatcgc cggctatgcg
    20221 ctcgatgccg gctgctcgat tctcatcgcc gtcaacaagt gggacgccgt gcccgacaag
    20281 ttgacgggga cgccggccga gttcgagcgc gacctgcgtc agaagatgaa gtttctcgaa
    20341 tgggcgcccg tcgtcaccat ctcggcgctc tcgggtcagc gcgtggacaa actgctggcg
    20401 ctcgccgtgc gggccgacga ggcgcgcgcc acgcgtatct cgacctcgca gttgaacgac
    20461 ttcttcgcgc gcgccatcgc cgccccgcac gcgcccaccg cgaaggcgca gggcgggcag
    20521 acgcgcatcc acgtccagta cgtcacgcag gtggcggtgc ggcccccgcg cttcgtcgtc
    20581 ttcacggccg gcggccgccc cggcctgcac ttctcctacg agcgctatct gcacaaccaa
    20641 ctccgcgaag agttcggctt tttcgcgacc cccttacgca tcatcgagcg acacaagaag
    20701 aagcagaggt cagagggtag aggttagagg tcagtgaaga gcaaaagtct ttaactaacc
    20761 tctaacctct aacctctaac ctctgacctc tttcctatgc ctgaagagtc ccacctgaga
    20821 acgctgttcg agcacattag cgacccggcc gtggtcgtcg gcggcgggga gttgttcgcg
    20881 gccgggggcg aggtgctggc ggcgaacgcg tcgttcgcgg cgctggcggg gcggccggag
    20941 tcggagttgt gtggagtcga ggtgtcggcg ttgctcgcgt ttgagggagg ggcgagtggc
    21001 ggttcttcat tcgggaatct gttcgcgggc gacggcgagg gttgggtgcc ggtcaaggtc
    21061 gagcgcgtgc cgtgccgttg ggagggacga gaggcgtgtg tcctgttggt gcgcccgacg
    21121 acggcggcgc gcgaccggga acacgagacg ctgcggcccg aggaagtcgg cgcgctcttc
    21181 gactttttgc aggaggccac cgagcacgtc gaggtcgtca accgcgtggt cgccgccgtc
    21241 aactccagcc gcaccatcga cgaggtcttc aagctcgcca gccagcagat cggcgcggtc
    21301 gtccccttcg accgcgcctc catcgctctc tgcgaggagg actgtaaacg gctacgagtc
    21361 ttcgcactct caggggagca cgccgccccg cttcaggtcg gcgcgaccgc cccgctgcgc
    21421 ggctccgtca ccgagcaggc gctcggcgag cgccgcgcct tagtcatccc cgacctcgcc
    21481 gccgagacgc gcttcaacgt ctacgacgac ttgcagcgcg agggcttccg ctcggccgtc
    21541 tgcgtgccgc ttttctcgat ggagcgcgcc gtcgggagcc tcaacctgac gagccgcacg
    21601 cccgcggcct acggccggcg ccacctgctc gccctcgaac gcctcgccgc cccgctcgcc
    21661 atcgccatcg agaaagttct gctcctcgaa cagtccgagc gccgcgcgcg cgagatgggc
    21721 gaccgcgccg aggagctgag cggcctcttc gagatttccc gaaccttctc cacgctcacc
    21781 gacaccacgg agatcagcgc ccgactggcg cgcgccatcg cacagctcgt cggcggcgag
    21841 acgtgcctca tcgccaccta cgaccgcaag cagaacgcgg tgcgcgccga ggccggacac
    21901 aacacgccgc ccgagctgct gcgcgagttc cacttcgcgc ttgagcgcga gaacacttcc
    21961 gcccccgtct acctgaccgg cgaggcgttc ctctcgaacg accccggccg ggacgcgcgt
    22021 ctcaaccact ccttcgtcga acgctaccgc atcaactcgg tgctgagcgt ccccatgcgt
    22081 atcaagcgcg agctgatcgg tttcatctac gtcggcaacc gcgcgggagg ctttcgcgag
    22141 cgcgacatgc gtctgctgga aatcttctcg gtgcaggcgg ccgagaccat cgtcaatgcg
    22201 cgcctgttcc tgaccattca ggcgcaggcc gagcgcgagg ccgtcatcaa ccgcctgctg
    22261 ctctcgcttc agcgcggcgg cgagccgcgc gacaagatcg gcgccgtcat cgagcgcatc
    22321 ggacaggtgc tcgacctcga ccgctgcatc gtcgtgctct tttccgaggg cgagcatgac
    22381 gactattacg gcgagtggtg cgccgaaggc gtcgcgcccg tcacgcacat ggcggacgtg
    22441 cgcgagcgtt cgcccgtccg ctacgcgcta cgcacggcgc ggcgtccttt gatcgccaac
    22501 gacgtgacga gtcacccgct cgccgccggc aactcagagc tcgtcgagcg cacccagctc
    22561 aagagcatga tggtcgtccc catcatgcac ctcgggcgcg tcatcggtag tttgagcgcg
    22621 caccagacgc gcgcccgacg cgagtggagc gaggacgaca tcgacctgct gacggcggtc
    22681 gccacgcacg tcggggcgac gctggagaac gcccggctca tcaccgagct gcgcgaggcc
    22741 aaccggctca aggacgaatt tctcgccacg ctctcgcacg agctacgcac tcccctgacg
    22801 gccatcaccg gctgggtcga tctgctctcg gaaagcgagt cggcacgcac cgaccgcgac
    22861 ctcgccgacg gcgtcgccgc catcagcgcc tcggccacgt cgctcacgca gctcatctcc
    22921 gacctcctcg acctgtcgcg catccagcgc ggcgtgctca gattggagcg cgagccgctg
    22981 gacgtgaacc agtcggtgac gagcgccgca cgggccgtcc ggcagggggc ggccgcgcgc
    23041 cggcaggagt tgcacctgga gcttgcggag agcctgccgc acaccatcgc cgacgcgcac
    23101 cgcgtccagc agattttgtg gaacctcctg acgaacgcca tcaagttcac gccgcagggc
    23161 ggacgcgtca cggtgcgctc gcggcttttg gacgaggggc tgaagctcgg cgagcagggc
    23221 gcggcgggag agcacctgcg ctggattgtg atcgaggtgg aggacacggg cgagggcatc
    23281 cccgaagagt tcctgccatt tatctgggat cgcttccggc aggccgacgg ctcggccacg
    23341 cgccgccacg gcggactcgg catcggcctc gcgctcgtca aggagttgat cgaggcgcac
    23401 ggtgggtcgg cggaggccga gagccgcgtc ggcggcggca cctccttcag cgtccgcctt
    23461 cccgtcatcg aggtcgagga gtggatggag agggagccgc gcttccagtc gccgcgaggc
    23521 gacggcgccg ggcgcggcga gttcagttag gaagctgagg gcgcgtcgca agactgtagg
    23581 ggcaggg
//
LOCUS       DASVES010000307        26610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1011222, whole genome shotgun sequence.
ACCESSION   DASVES010000307 DASVES010000000
VERSION     DASVES010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 26610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26610
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011222"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1210
                     /locus_tag="VF240_12415"
     CDS             278..1210
                     /locus_tag="VF240_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176056.1"
                     /translation="MTTVTNQRGIRERFARVMALGLLAFAAVLCSAPEARAQWATNGN
                     NINNTNTGNVGIGTTTPAAIVDMQRMDTSDLLMRLWNTNPGASTATFRIANSSNAGSR
                     LQFTDSNLYVSSIFAERTGGLRFRTGNASSEAALVDRMAILPGGNVGIGTTAPTARLH
                     VTGDIRVDGNISAKYQDVAEWVPTTQKLHAGTVVILDIEHNNHVTTTAVAYDTRVAGV
                     VSAQPGISLGEAGEGKALIATTGRVRVKVDATRAPIRVGDLLVTGDTPGVAMRSQPLD
                     LGGVPIHRPGTLIGKALEPLDKGTGEILVLLSLQ"
     gene            1246..2430
                     /locus_tag="VF240_12420"
     CDS             1246..2430
                     /locus_tag="VF240_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176057.1"
                     /translation="MMTTTENRGRSLGVRLCRAATFSLLAFAATLYSASEARAQWATN
                     GNHITNTNPGNVGVGTMNPAQIFHVHSPGTWAGTRVTTGATGATVGDGVNFGYDDSVG
                     AYIWNREASPVVFSTSNTERLRIDATGKVGIGTITPGSQLHVEASDAGAFSSAALGGS
                     RLIIQNNNLTNGTYSDISFRTLDSSGFGQTTARITGIFTNHAFGGVSGDLAITTMNTM
                     TRNEVARFTGSGNVGIGTAAPQAKLHVTGDIRVDGNISAKYQDVAEWVPSTQQLSAGM
                     VVVVDAEGVNHVLASASAYDTGVAGVVSAQPGIILGEGGEGKVKVATTGRVKVRVDAT
                     RAPIRAGDLLVTSGASGVAMKSVPVSVGGVSMHRPGTIIGKALESLEKGTGEILVLLS
                     LQ"
     gene            2579..8221
                     /locus_tag="VF240_12425"
     CDS             2579..8221
                     /locus_tag="VF240_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176058.1"
                     /translation="MTVSHPDEALAAAAVAAPASLAVTSTSSSQINVSWTAATGATGY
                     KVERAQTAAGPYTLVGAPTTTTYADTAVTAGTTYVYRVRATDAAGNLSAYSNTAVGTA
                     MTFTDPTLVSGVTPIKAVHMTELRQSVNSVRAAAGLGGVTWTDPTLAGATVRGVHVQE
                     MRDSLNQALTNLQVAVPAYTDPTLFTGASGTTIKRVHVEELRRFSASGTSSYVPPSGD
                     DDLPTAACPAPQSWPADGATIVANVVALDQVFFYNRLGAFNPAGMIFALERDVVKIDD
                     SKPLGPGNAQLRRDKRPRPITLRVNIGQKLQINFKNWLGHQPADDNQPHTRAASVHVM
                     GLALVGSIASDGSNVGRNTSSLVTPGNATTYTYYAEREGNHLLYSTGATTGGEGDGGS
                     LAMGLFGSVNVEPRGAEWYRSQVKATELKFATKLKADGTPDMIPDTTGAVSATRPKIC
                     YDAVYPAGHTRAGQPVLKMVQGNEIVHTDLNALITGPGKGNFSPNTYLPNPTEPDRNQ
                     PFREFTVVYHDEIKAVQAFPQFNDKLKNPSTGEYDIDNPLAHTLHSVRDAFAINYGTG
                     GIGAEILANRFEVGPMANCTECLYEEFFLSAWSVGDPAQIVDKPANGGSDDTGKLPTP
                     GPKANKVLFPEDPSNVHHSYLNDHVKMRVVHAGPKEHHVHHLHAHQWLHTPDSDNSTY
                     LDSQAMGPGYSFTTEITHGGSGNRNKTVGDSIFHCHFYPHFAQGMWELWRTHDVFEAG
                     TKVDGAGVPTVGSRALPDGEIRAGTPIPALVPLPTIAMAPAPGAQVSIVNGQPQVSGA
                     GNPGFPFFVPGVAGHRPPHPPLDSIDDGGLPRHVIVGGVAVHHEEKLAQFSLAKARLD
                     FDKEVEVADARAVPEAGTPIEQQAMVFHEKPSHPSFKPDGASASFTTNGMPRIAGAPY
                     ADPCRPQDGFTSTRLYKSANIQIDATFNKAGWHFPQQRISVLWEDVAAITGGQRPPEP
                     LFFRANTNECIEFHHTNLVPKVYKLDDFQVRTPTDVMGQHIHLVKFDVTASDGAGNGW
                     NYEDGTFSPEEVLSRIKAINAGGGLLQFGGNSRTKLSPEMHPLGVLGAQTTVQRWFAD
                     NVLNNLGKDRTLRTVFTHDHFGPSTHQQVGLYAGLVIEPTGSTWRHPETGQMFGGRPD
                     GGPTSWRADILWINPETNKDESYREFLLEFSDFQLAYKKGGGGTVNNPHPDPANVINP
                     PAKNEPGLPVLVEPAQQCPGSNFAPPCPEAVSAADPGTAVVNYRHEPIPLRIVNDPNV
                     SNPTQAGGEQGDLSHVFRSDITRAYAPMNSHADVPPLTLDVKPGDPATPLLRAYEDDK
                     VQVRVLVGGFEEGHNFGVQGMRWLFEPSDPNSGFRNNQMMGISEHYEFEIPRFPRSTK
                     TVSDKKFVDYRYAPGSAVDDLWNGLWGLLRAYDAKTNSVDDVLLELPNNMKSTMDKLT
                     DRIGDKSDFNGSCPKTARVRVFDVTARLAKDALPEGTLVYNPRTNQGGRLHDPTAIMF
                     FHTADLANGVFWDADRKVEPLILRANAGDCIELTLRNQLPDATPPDLDGFNTLPMIVN
                     HFNANEIDPSKEVGLNPQLVFFDVSRDNGMNIGHNPVQTVKPNQMKTYQWYAGAVTIV
                     TQPDGSKKWVGTPAEFGATNLSSSDPVKHSNKGAIGALIIEPETAAWVDSEWVPNPDP
                     TSARTQMKTRAQATITPYGGASFREFVLLFQNDINLRRGDGLGTAVPNTAEAEDAEDS
                     GQKAFNYRTEPMWKRENFEPDTPLNQTREEDFSDSLSNSIVGGDPVTPVFTARAGQPV
                     RFRVLHPGGHARNNVFQVHGHIWEEEPYTNSSTVLGSNLFSEWKGSQYGVGPGSHFDM
                     LLKNGAGGKFGIVGDYLFRDQGSFPFSGGLWGIFRVTP"
     gene            complement(8364..10169)
                     /locus_tag="VF240_12430"
     CDS             complement(8364..10169)
                     /locus_tag="VF240_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176059.1"
                     /translation="MSIWQIIRRLVPFVRPYSRWVFLSLALTLLGAFAAQVNPIVLRY
                     TVDTIERLLREGRTASQGTSLLLLISAVLLGKEIINTGLRYGQNLFGEKIRVNVSSRL
                     SQFAVEKILTYRLAFYSATDNQTGILQTRIDRGAESLTKLVQSAFIDILPLFANAILA
                     LIIMFLANVYVGLVASAILPVYFLLSQRQASMLKGARRGLRSLREEKNNGLHNIIESI
                     IVIKSFVREEYEKEKQVRLQQNLVGAQLKQRQTNFVFDGLKTFVEQMGVVLIIILTAY
                     LVLGGWMSIGAIMFHIMLFGNVSAPIRQLHRIYDEMNDALTYAEGYFQILDADDQIEA
                     SGELDVSHLEGTFELIDVSFTYPNGTQALHHVNMKIPHGKTVAFVGLSGAGKSTLLNL
                     LTRFYTPTSGRILLDGKELDQYETDLLRRKIGLVLQKNHIFKGTIEENIRYGKIDAPF
                     SEVVEAARKAYLHEQVEHLPNKYESEAHLLSGGQQQRIAIARLFLKDPPVIFLDEPTA
                     SLDAVATEQIKNSLDAIKEERTTVIISHSISQIIDADIIFVLKEGRVVEHGAHEDLYE
                     RDGTYREIFNASARSLNIDKISRTLDSALAPDLIN"
     gene            10305..11396
                     /locus_tag="VF240_12435"
     CDS             10305..11396
                     /locus_tag="VF240_12435"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEX7176060.1"
                     /translation="MHAFTRRTPLSLIGVVAFACLVASACSGRASGELRGRGAQQKGF
                     EGLYRIRKDGKTGFIDRAGKVVIPPQSDLKVPDYGTYPVFTEGLAQIEVETDEPGPGT
                     FSNKKKIGFIDTTGKVVIEPRILQGAYDFSEGVAAVSLNYHYGFIDKTGRVVIPIEWQ
                     SARSFAEGLAAVGRDAQFGFIDKAGKAVIPLQFDGAQSFSEGLAAVIVNRGKVFIDKT
                     GRVVLEPQVDDAQSFSEGMALISVNGKRGFIDKTGQVVIQPIYAVAQDFSDGMALVRV
                     DGKVGYIDKTGAVVIKPQYELAFDFSEGLAPVKIGDSWGYIDKTGRVVIEPRFYSAYE
                     FRGGLASVTTSEGDGYIDKAGKYIWPPSK"
     gene            complement(11824..15474)
                     /locus_tag="VF240_12440"
     CDS             complement(11824..15474)
                     /locus_tag="VF240_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176061.1"
                     /translation="MKFREIFRFEFAYQVRRLQTWLYFAALVVVAFLFVRGNHLADAL
                     YADFYLNSPFVIASVTVFAGLFWFVVAAGVAGETAARDVETGMHPLTYTAPVSKAEYL
                     GGRFLAGFVLNALILLAVPVGVMLAVYSPGVEAEVVGPFRPAAYLTAYGFIALPNAFV
                     GSAIQFAWAALSRRAIASYVGSVLLFFVAYGGMFFAAIFLERQDLAALLDVFGHVFIT
                     SDLILGWTPIEKSTRLIELEGPLLWSRLVWFGIALGTLAFTYLRFRFAHPTASHRWSR
                     LTRRREAHAPTPPLNDIARSMISMPQFRQTFGSATQLRQTLAIAWDSFRTIAKSRGGL
                     VLLAAIAALAVVVMPENMSNMGTPLLPRTEYVLTFLTARLTNPLTPWVITPLLIVFYA
                     GELVWRERKAGLGEITDAAPVPEWVSFLGKFLGLGLILVAWMALLTMAGVLVQTRMGY
                     DDFETGLYLRVLFGLQLPEYLLFALLALVVQGLVNQKYVGHLVALIAYASILFASSLG
                     IEHNLLVYGSAPDWSYTDMRGFGASLGPWLWFMLYWAAWALLLAVVARLLWVRGMEGG
                     LRVRLQLARRRFTRPTAGAAAAAVGLVLTLGGFIFYNTNVLNEYTTASGKMERRAEYE
                     RRFGQYANVPQPRLTGTNLHVEIYPRRREVEIRGAYQLVNGAAAAIDSIHVATDSGVE
                     TGAVAFDRPVARVLADENLGHRIYVLEEPLQPGDSLRLSFEVHFKPKGFRNSGGGASV
                     AANSTYFKNDWLPAIGYQPRRELFKPGERRAHGLAPRPLFPSLADAEAGQDITGEAGD
                     DLTGAERIAFEAVVGTDGDQTAVAPGALRRTWTEGGRRYFHYATDTPIGNEHRFFSAR
                     YAVHEEQWNDPSSGSGRVVAIQVYHHPRHAANLNRMLRSVRASLDYYTRAFGPYPYGY
                     IRLIENPGRGIGAHADATTIDYTEGFSRFNPADDSRGLDLPFAVMAHEMAHQWGVPYA
                     FAEGAPLLSESFAWYAAMGVVEETYGREHLQRLRRFFRQPSPIPPIRQSVPLLRAMDP
                     YAAYRKGPFALFALSEYMGEERVNLAFRRLIEKHRLGAPPLATSLDLYRELQAVTPDS
                     LRYLLHDLFEANTFWELETERATAEQTDAGAWQVTLRVRARKVTVDPAGVETEAPMDE
                     WVPIGVFAPTEKQGADFGETLYLQMHRIRSGEQTITVTVPREPSDAGIDPYHLLIDLE
                     RFDNVEEVKIES"
     gene            complement(15471..16382)
                     /locus_tag="VF240_12445"
     CDS             complement(15471..16382)
                     /locus_tag="VF240_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176062.1"
                     /translation="MELHIRDVSKTYPNGVQALRDVTLTIPVGMYGLLGPNGAGKSTL
                     MRILATLQEPDTGAIHLGDLDVVQQKDAVRQTLGYLPQEFGVYPKVSAEALLDHFAIL
                     KGIPDKKARKEVVEALLRQTNLWEVRRQKLGGYSGGMRQRFGVAVALLGNPKLLIVDE
                     PTAGLDPAERVRFLNLLSELGENSAVILSTHIVEDVSELCTRMAIIDRGEILLEAEPL
                     GAIEEMRGRIWRSVIEKGALAEVERAHAVISTKLLAGRTVVHVFSDAAPDPGFEPAEP
                     DLKDVYFSTMAGHIGRRREQPEPEMAP"
     gene            complement(16595..17419)
                     /locus_tag="VF240_12450"
     CDS             complement(16595..17419)
                     /locus_tag="VF240_12450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX7176063.1"
                     /translation="MAHTVGTDAVGLSAPRVTRDFVAPGSVVRNIWGDPELVLLVFAG
                     SAAEFALNRAVDWLFFTGGIPNDPVGRLFSTVRYAQEIVFADEDTARRTLGRINAIHG
                     AVESQRGQRIPDWAFRDVLYMLVDYSERAHRLLYGPLRAAEREELYAVFRRVGEGLNI
                     GGLPEGYEQWRGDRQAHLERDLAFSRHTALLYEQYRRHLGAWRYQILLGVQALLAPDL
                     VRRLLRLRRRAVFSALVGGYSHLEVPGLRPLVRRLLVQPRYWGEVDKFDRGSPAGV"
     gene            complement(17489..18493)
                     /locus_tag="VF240_12455"
     CDS             complement(17489..18493)
                     /locus_tag="VF240_12455"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7176064.1"
                     /translation="MVKRLFLVFQTFSLIALASAIAIAQNGQSSRSNAQPEQIVPAAD
                     HHQHLFSQARVDFQPSGLKTITAQDIIGLLDQAGIRRAVLLSTAFGYGRPGSDPPDEY
                     AKVKAENDWNAAQAALFPKRLIAFCSFNPLKEYALDELARCAKDANLRHGIKLHFGSS
                     DVQTEVPEHFEKLKKVFQAANSQRMAIIVHMRASISKKRPYGPEQARAFLELLSFAPD
                     IPVQIAHLASSGPGYDDPPAHSVIEVLADAVAKKDSRTRKLWFDVATNAHPTNSAEVS
                     ELLVKLIRQIGVKRILYGSDSALGNNLRPRESWEAFRQLKLSTKEIKTIAGNVAPYFR
                     "
     gene            18701..19435
                     /locus_tag="VF240_12460"
     CDS             18701..19435
                     /locus_tag="VF240_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7176065.1"
                     /translation="MAESIEHQMTEIYCFVDDYLVAHPALLRWRRSPHACPRFTDSEV
                     ITIALLQGPLGVASLKKAYRMVAKNWGAAFPALPTYTQWMHRLHQLTRQVGALLEATC
                     GHDAAQARLYLMDSKPIPLCHVLRHGRVRLLREDGARFGKTSKGWFFGFKLHAVRHIA
                     GRVMNIILTPANWDDRDPAPALSEGTDGGIAIADLGYRGPKCAASLEGAEGLLITRAD
                     APERKFLLSSQARRRDDVQSVVASVH"
     gene            complement(19704..20561)
                     /locus_tag="VF240_12465"
     CDS             complement(19704..20561)
                     /locus_tag="VF240_12465"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HEX7176066.1"
                     /translation="MSRTILFEGQPPKNITGKNGAAQARVRVRTVALPTEHGGWGLML
                     EPVVLGLLVAPSVPGLFIAVATMGAFLARHPFKIVAGDRRRGRRFPRTAVAERFTLLY
                     AAVAVAGLLAASVSAVSHQFFLPLLLAAPFAAVQLKFDATGRSRALAPEVAGSLGLAA
                     VSASIALADGWSLPAAAGLWAVLAARVVPTILYVRARLSLKRGETPGRSPVIIAHVCA
                     LMAVAALVFMRLLPILAVAALSVLFLRAVIGLSRNPQLASAKQIGIRELGYGVMTVLA
                     VAAGHLIAR"
     gene            complement(20596..22188)
                     /locus_tag="VF240_12470"
     CDS             complement(20596..22188)
                     /locus_tag="VF240_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEX7176067.1"
                     /translation="MNSPKLRTFFCALLLLCAAPGIQSARAQEPALADNKMNASGDEK
                     TRTLAPPSATTSKPAAAPPAPKQLKIGGVNLSGSLRLRVENYGWFKTPGYEDEYTFGA
                     AVLRLSVGQQKEKFDWLVEGEFPVLMNVPERSVAPAPQGQLGLGGSYFAANGRQDASA
                     VLKQAFVRVKGLFGDKASSLRVGRFEFVDGMETTPSDATLAALKRDHVAHRLIGHFGF
                     SHVGRSFDAVQYVRNTKSGNFTFVGGRPTEGVFQLRAVKELDVDFWYGAYTKPLAGER
                     VTSELRVLALHYHDGRGALKTDNRAPALRRADAENIRLTTVGGNYVAAVKTARGTVDL
                     LAWGVGQFGRWGNQGHRAGALVLEGGLQPAAKFAARMRPWLRAGYSRSTGDGDPTDAR
                     HTTFFQVMPTPRWYARFPFYNMMNNEDVYAQLKLRPHARLALRAEVHHLRLSNRKDLW
                     YVGGGAFQESSFGYVGRSSGGRKSLGTLFDLSADVTVTPTTTLTLYGAGVRGGAVQSS
                     IHPAGGANPAARFFYAELSKRF"
     gene            complement(22193..23419)
                     /locus_tag="VF240_12475"
     CDS             complement(22193..23419)
                     /locus_tag="VF240_12475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7176068.1"
                     /translation="MSETFKPGAARALFLSTTAFAVSFAVWGLVAALAPTFTQMYDLS
                     AKAKSLMIAVPVLLGSLGRLPAGMLADRFGGRRVFAALLVLSALPAVGIAFSTSYAQL
                     LLLGLFLGMAGTTFSVGVGFTSKWFAPEKQGAALGVFGMGNIGQSIAVFGAPVLALAL
                     GDWRTVFYIFAAAALVWGVVFYLFARDAAVTTKPKTLAENLNVLRRSPLAWVLSLFYF
                     VTFGGFVAFSIYLPTLLRDQYGLTPTDAGARTAGFVLLATLLRPVGGLLADRFGGARV
                     LLGVFALVAFFGAMMGCPWFVTFSVGALGAAAALGLGNGAVFKLVPQHFPKETGTVTG
                     LVGAFGGLGGFFPPLVLGVIRDSFGVYWPGFILLAAFALLCLAVNYFVFLKPRNGTRK
                     EETPSLSARAMNMRGA"
     gene            complement(23608..23811)
                     /locus_tag="VF240_12480"
     CDS             complement(23608..23811)
                     /locus_tag="VF240_12480"
                     /inference="COORDINATES: protein motif:HMM:NF023510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HEX7176069.1"
                     /translation="MKRRIAGFHTDDEGHWRAELECGHPQHVRHDPPLTSRPWVLTEE
                     GRASRLGLELDCKRCDEEARGED"
     gene            complement(23837..24136)
                     /locus_tag="VF240_12485"
     CDS             complement(23837..24136)
                     /locus_tag="VF240_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176070.1"
                     /translation="MPSERATQVQVSRRGAGRGHAAVFQARLILLVMINVAQLWILSA
                     AVEAALARHYSLLVPLVVASGVCWLIALSIVLWWRPVSRRYTSTGYVRESAPQRK"
     gene            complement(24126..24716)
                     /locus_tag="VF240_12490"
     CDS             complement(24126..24716)
                     /locus_tag="VF240_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX7176071.1"
                     /translation="MKNLNETIKRVMTDDGGGVSDERDVREATARSQHDFPYGRGEEA
                     QVTRREFCNFLALTSTALFVSAAGFAAKAALDARASVSFAPSRVEGAESLAPGSAMNF
                     LYPSEKDTAILVRDAEGQYHAYGQKCTHLACPVYYERAHARFECPCHEGAFDIKTGNV
                     LYGPPPRPLDRIELEVRDGGEVWATGRVPGGGEHAI"
     gene            complement(24793..25449)
                     /locus_tag="VF240_12495"
     CDS             complement(24793..25449)
                     /locus_tag="VF240_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7176072.1"
                     /translation="MEETMFIDPQRCIGCRSCVAACRECGTHKGYSMIYVDYLDRAET
                     TATMPTVCMHCNEPTCALVCPADAIKVSEEGVVMSALKPRCLDCRNCVNACPFGVPKY
                     NSAMHLQMKCDMCFDRTSEGLKPMCASVCPTGAISFGRYEQIVPLRRTKPVNVHVFGN
                     QKVETKVFLMLPTDAKQVDVDGCGVFEGQLSLAEGGHTPTAGSWIDTQVEESDKSNEF
                     "
     gene            complement(25590..>26610)
                     /locus_tag="VF240_12500"
     CDS             complement(25590..>26610)
                     /locus_tag="VF240_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX7176073.1"
                     /translation="ELPQAGVSVVEMFQKMREGEIRGLLSICNNVMVSLPDTNEVRRS
                     LEGLDFYVCIDFFMSEGARYADVVLPGTTWSEDEGVTANSEGRVLKINKAADPPGEAR
                     VDWWIIQEIARRMGRGKYFSFNSPREIFDELRVASRGGNADYYGISYEKIERQNGVFW
                     PCPTEDHPGTPRLFEEKFYHPDGRAKFHPVEYNPPAEVPDEQYPLILTSGRVVYQYLS
                     GNQTRRIGFLVEQCPEPYVEIHPETAARLKVNDGERVKVVSRRGEGVFPALVVRTIRP
                     DTIFIPYHWGEELAANQLTNPALDPTSKIPEYKACAARIEKIHSRRLPILGERRKGTS
                     RSEVR"
BASE COUNT         5077 a         8607 c         8418 g         4508 t
ORIGIN      
        1 cgtgatacat ggtgaaacac tacgcgctcc ctcgtgccgc acgctgaaac atccccgccg
       61 agatgttcaa cctcgaaact tcataatcac atagttattg aaccgtaatg aacagggcac
      121 ggcgcttgct aatcgcaaca ccgctgcaag aattcacgcg accgttcgga ggttgtccga
      181 cgcgaccgca accgacgacg aaagattctt tcggcatcgc gtgcgcctca gccttcggta
      241 gctcactact catacgaaca gaaagagaag agacgacatg acgactgtga ccaatcaacg
      301 agggatcagg gagcggttcg cccgcgtgat ggcgctcggg ctgctggcct tcgcggcggt
      361 gctctgctcg gcgcccgagg cccgcgcgca atgggcgacg aacggcaaca acatcaacaa
      421 caccaatacg ggcaacgtcg gcatcggcac gacgacgccg gccgccatcg tcgatatgca
      481 gcggatggac acgtcagacc tgctgatgcg gctgtggaac acgaaccccg gcgcgagcac
      541 cgcgaccttc cgcatcgcca actcctcgaa cgccggcagc cggcttcagt tcaccgactc
      601 gaacctgtac gtgagttcga tcttcgccga gaggacgggc gggctgcgct tccgcaccgg
      661 caacgcctca tccgaggcgg cgctcgttga ccgcatggcg atcctccccg gcggcaacgt
      721 cggcatcggc acgaccgcgc cgacggcccg gctgcacgtc accggcgaca tccgcgtgga
      781 cggcaatatc tcggccaagt atcaggacgt ggccgaatgg gttcccacca cgcagaagct
      841 ccacgcgggc acggtcgtca tcctcgacat cgagcacaac aaccacgtta cgaccacggc
      901 cgtcgcctac gacacgcggg tggccggcgt tgtctcggcg cagccgggca tctcgctcgg
      961 cgaggccggc gagggcaagg ccctcatcgc cacgacgggc cgcgtccgtg tcaaggtcga
     1021 tgcgacgcgc gcgcccatcc gcgtcggcga cctgctcgtc accggcgaca cgccgggcgt
     1081 cgccatgcgc tcgcagccgt tggatttggg cggcgtgccc attcatcgcc ccggcacgct
     1141 catcggcaag gcgctggagc cgctcgataa gggcacgggc gagattctcg tcctgctcag
     1201 ccttcaatag ttctacggcc acacgaggaa aaggaaagta acgacatgat gacgacgact
     1261 gagaacagag gacgaagcct cggcgtgcgg ctctgccgcg ccgcgacgtt cagcctgctc
     1321 gcctttgcgg cgacgctcta ctcggcgagt gaggcgcgcg cccagtgggc gacgaacggc
     1381 aaccacatca ccaataccaa tccgggcaac gtcggtgtcg gcacgatgaa cccggcgcag
     1441 atcttccacg tccacagccc cggcacatgg gccggcacgc gcgtgacgac cggcgcgacg
     1501 ggggcgacgg tcggcgacgg tgtgaacttc ggctacgacg acagcgtcgg cgcctacatc
     1561 tggaacaggg aggcgagccc tgtcgtcttc tcgacgagta acaccgagag gttacgcatc
     1621 gacgccaccg gcaaagtcgg catcgggacg attacgcccg gctcgcagtt gcacgtcgag
     1681 gccagtgacg ccggagcctt ctcgtcggcc gcgctgggcg gctcgcgtct gatcattcag
     1741 aacaacaacc tgacgaacgg cacttacagc gacatctcct tccgcaccct cgacagcagc
     1801 ggcttcggcc aaacgaccgc gcgcatcacc ggcatcttca cgaaccacgc cttcggcggc
     1861 gtctccggcg atctggccat caccaccatg aacacgatga cccgcaatga ggtcgcccgc
     1921 tttaccggca gcggcaacgt cggcatcggc acggccgccc cgcaggccaa gctgcacgtc
     1981 accggcgaca tccgcgtgga cggcaacatc tcggccaagt atcaggacgt ggccgaatgg
     2041 gtgccctcca ctcagcagct ctcggccggc atggtcgtcg tcgtcgatgc cgaaggcgtc
     2101 aaccatgtgt tggcgtcggc atcggcctac gacacgggcg tggccggcgt cgtctccgca
     2161 cagcccggca tcatcctcgg cgagggcggc gaggggaagg tgaaggtggc gacgacgggc
     2221 cgcgtcaagg tcagggtcga tgcgacgcgt gcgcccatcc gggccggcga cctgctcgtg
     2281 acgagcggcg cgtcgggcgt ggcgatgaag tcagtgccgg tctcggtcgg cggagtgtcg
     2341 atgcatcgtc cgggcaccat catcgggaag gcgctggagt cgttggagaa gggcacgggc
     2401 gaaatcctcg tcctcctcag tctccagtaa cgctggggtc gcgtccattt ttccgcgatg
     2461 gcaaacagct actactcacg aacaggtaaa aagaggaact gcgatgaaaa gcgtagagcg
     2521 attggggcgc gggtggtcaa cgtcaaagtg cgtagccatg ctcgtgttgg ccctgttaat
     2581 gactgtgtcg catcctgacg aggcgctggc cgccgccgcc gtcgcggccc cggcgagtct
     2641 cgccgtcacc tccacctcaa gctcacaaat aaatgttagc tggacggccg ccaccggcgc
     2701 gaccggctac aaggtcgagc gcgcccagac ggccgcgggg ccttacaccc tcgtcggcgc
     2761 cccgaccacg acgacctacg ctgacacggc agtgaccgcc ggcacgactt acgtctaccg
     2821 cgtgcgcgcc accgacgccg ccggcaacct gtccgcctac agcaacaccg cggtggggac
     2881 ggccatgacc ttcaccgacc ccacgctcgt gtcgggcgtg acgcccatca aggcggtgca
     2941 catgacggag ctgcgccaga gcgtcaactc cgtccgcgcg gcggccggcc tcggcggcgt
     3001 cacctggacg gaccccacgc tggccggggc gaccgtgcgc ggcgtgcacg tgcaggagat
     3061 gcgtgattcg ctcaatcagg cgttgacgaa tcttcaggtc gccgtgcccg cctacaccga
     3121 cccgacgctc tttaccggcg cgtccggcac caccatcaag cgcgtccacg tcgaggagct
     3181 gcgccgcttt tcggccagcg ggacgagttc ctacgttccg cccagcggcg acgacgacct
     3241 gccgacggcc gcgtgccctg cgccgcagtc gtggccggca gacggcgcga cgattgtcgc
     3301 caacgtcgtc gccctcgatc aggtcttctt ctacaaccgc ctcggcgcgt tcaacccggc
     3361 cggcatgatc ttcgccctcg agcgcgatgt cgtgaagatc gacgacagca agccgctcgg
     3421 ccccggcaac gcacagttgc ggcgcgacaa gcgcccgcgc ccgatcacat tgcgagtcaa
     3481 catcgggcag aagctccaga tcaacttcaa gaactggctg ggccaccaac cggcggacga
     3541 caaccagccg cacacgcgcg ccgcctcggt acacgtgatg ggtctggcgc tcgtgggcag
     3601 catcgcctcc gatggctcga acgtcgggcg caacacgtcg agcctcgtca cgcccggcaa
     3661 cgcgaccacc tacacctact acgccgagcg cgaaggcaac cacctgctct acagcaccgg
     3721 cgcgacgacc ggcggcgagg gcgatggcgg ttcgctcgcc atgggtttgt tcggctccgt
     3781 caacgtcgag ccgcggggcg ccgagtggta tcgcagtcag gtcaaggcca cggagctgaa
     3841 gtttgcgacc aaactgaagg ccgacggcac ccccgatatg atcccggata cgacaggtgc
     3901 ggtgagcgcc acacggccga agatttgcta cgacgccgtc tacccggcgg gccacacgcg
     3961 cgccggccag cccgtgctga agatggtgca gggcaacgag atcgtccaca ccgacttgaa
     4021 cgccctgatc acggggccgg gcaagggcaa cttctcgccg aacacctacc ttcccaaccc
     4081 gaccgagcct gaccgcaatc agccgttccg cgagttcacg gtcgtctacc acgacgagat
     4141 caaggccgtg caggctttcc cgcaattcaa cgacaagctg aagaacccgt ccacgggtga
     4201 gtacgatatc gacaaccctc tggcgcacac gctgcacagc gtccgcgacg ccttcgccat
     4261 caactacggc acgggcggca tcggcgcgga aatcctcgcc aaccgcttcg aggtcggccc
     4321 gatggcgaac tgcaccgagt gtctctacga agagttcttc ctctcggcgt ggtccgtcgg
     4381 cgaccccgcg cagatcgtcg ataagcccgc caacgggggt tcggacgaca cgggcaagct
     4441 gcccacaccg gggccgaagg ccaacaaggt gctcttcccc gaagacccgt cgaacgttca
     4501 ccactcttac ctgaacgacc acgtcaagat gagggtcgtc cacgccgggc cgaaggagca
     4561 ccacgtccac cacctccacg cgcaccagtg gctccacacg cccgacagcg acaactcgac
     4621 ctatctcgac tctcaggcca tggggccggg ctactccttc accaccgaga tcacccacgg
     4681 cggctcgggc aatcgcaaca agacggtggg cgactcgatc ttccactgtc acttctaccc
     4741 gcacttcgcg cagggcatgt gggagttgtg gcgcacacac gatgtcttcg aggcgggcac
     4801 gaaggttgac ggcgcgggcg tccccaccgt cggctcacgc gctctgcccg acggagagat
     4861 tcgcgccggc acgccgatcc cggccctcgt gccgctgccg accatcgcca tggccccggc
     4921 tccgggcgca caggtgagca tcgtcaacgg tcagccgcag gtgtcgggcg ccggcaaccc
     4981 cggcttcccg ttcttcgtgc ccggcgtcgc cggccaccgc ccgccgcacc cgccgctcga
     5041 ctccatcgac gacggcggcc tgccgcgtca cgtcatcgtc ggcggcgtcg ccgtccacca
     5101 cgaagagaaa ctggcccagt tcagtctcgc gaaggcgcgg ctcgacttcg acaaggaagt
     5161 ggaagtggcc gacgctcggg ccgtccccga ggcaggcact cccatcgagc agcaggcgat
     5221 ggtcttccac gagaagcctt cgcatcccag cttcaagccc gacggcgcgt cggccagctt
     5281 cacgaccaac ggcatgccgc gcatcgccgg ggcgccctac gccgacccct gccggccgca
     5341 ggacggcttc acctcaacgc gtctctacaa gtcggccaac atccagatcg acgcaacgtt
     5401 caacaaggcc ggctggcact tcccgcagca gcgcatctcg gttctgtggg aggacgtggc
     5461 ggccatcacc ggcggacagc gtccgccgga gccgctcttc ttccgcgcga acacgaacga
     5521 gtgcatcgag ttccaccaca ccaacctcgt gccgaaggtt tacaagctcg acgactttca
     5581 ggtgcgaacc ccgaccgatg tcatgggcca gcacatccac ctcgtgaagt tcgacgtgac
     5641 ggcctcggac ggcgccggca acggctggaa ctacgaagac ggcacgttct ctcccgagga
     5701 agtgctctcg cgcatcaagg cgatcaacgc gggcggcggg ctgctccagt tcggcggcaa
     5761 ttcgcgcacg aaactgtcgc ccgagatgca cccgctgggt gtgctgggcg cgcagacgac
     5821 cgtgcaacgc tggttcgccg acaacgtctt gaacaacctc ggtaaggatc ggacgttgcg
     5881 caccgtcttc acgcacgacc acttcggccc ctcgacccac cagcaggtgg gcctctacgc
     5941 cggcctcgtc atcgagccga ccggctcgac gtggcgccat cccgagacgg gccagatgtt
     6001 cggcggccgt cccgacggcg gcccgacgag ctggcgcgcc gacattctct ggattaaccc
     6061 ggagaccaac aaagacgaga gctaccgcga gttcctgctg gagttctccg acttccagct
     6121 cgcttacaag aagggcggcg gcggcacggt aaataacccg caccccgacc cggccaacgt
     6181 catcaacccg ccggccaaga acgagccggg cctccccgtc ctcgtcgagc cggcccagca
     6241 gtgccccggc agcaacttcg cgccgccgtg ccccgaggcc gtctcggcgg ccgaccccgg
     6301 cacggccgtg gtcaactacc gccacgagcc gattccgctg cgcatcgtca acgaccccaa
     6361 cgtgtcgaac ccgacgcagg ccggcggcga gcagggcgac ctctcacacg tcttccgctc
     6421 ggacatcaca cgcgcctacg cgccgatgaa ctcgcacgcc gacgtgccgc ccctcacgct
     6481 cgacgtgaaa ccgggcgacc cggccacgcc gctgctgcgc gcctacgagg acgacaaggt
     6541 gcaggtgaga gtcctcgtcg gcggcttcga ggagggacac aacttcggcg tgcaggggat
     6601 gcgctggctg ttcgagccgt ccgatcccaa ctcgggcttc cgcaacaacc agatgatggg
     6661 catctccgag cactacgagt tcgagatacc gcggttcccg cgcagcacca agaccgtctc
     6721 ggacaagaag ttcgtcgatt acaggtacgc gcccggctcg gcggtcgatg acctgtggaa
     6781 cggcctgtgg ggcctgctgc gcgcctacga cgccaaaacc aactcggtag acgatgtgct
     6841 cctcgagttg ccgaacaaca tgaagagcac gatggacaag ctcacggaca ggatcggaga
     6901 taagagcgac ttcaacggct cctgcccgaa gacggcgagg gtgagggtct tcgacgtgac
     6961 ggcccggctc gccaaagacg cgctgcccga ggggacgctc gtctacaacc cgcgcaccaa
     7021 tcagggcggc cgcctccacg acccgacggc catcatgttc ttccacaccg ccgacctcgc
     7081 caacggcgtc ttctgggatg ccgaccggaa ggtcgagccg ctcatcctgc gggcgaacgc
     7141 cggcgactgt atcgagctga cgcttcgcaa ccagctcccg gacgcgactc cgccagacct
     7201 cgacggcttc aacacgttgc cgatgatcgt caatcatttc aacgccaacg agattgaccc
     7261 gtcgaaggaa gtgggcctca atccgcagtt ggtcttcttc gacgtgagtc gcgacaacgg
     7321 catgaacatc gggcacaacc cggtgcagac cgtgaagccc aaccagatga agacctatca
     7381 gtggtacgcg ggcgccgtga ccatcgtcac ccagccggac ggctccaaga agtgggtcgg
     7441 gactccggcc gagttcggcg cgacgaacct ctcgtcgtcc gaccccgtca agcacagcaa
     7501 caagggggcc atcggcgcgc tcatcatcga gccggagacg gccgcgtggg tggattccga
     7561 gtgggttccg aaccctgacc cgacgagtgc gagaacacag atgaagacgc gggcgcaggc
     7621 gaccatcacg ccttacggcg gcgccagctt ccgcgagttc gtcctgctct tccagaacga
     7681 catcaacctc cgtcgcggcg acggcctcgg cacggccgtc cccaacacgg ccgaggccga
     7741 ggacgccgag gactccggtc agaaggcgtt caactaccgg acggagccga tgtggaagcg
     7801 cgagaacttc gagccggaca cgccgctcaa tcagacccgc gaggaagact tctccgactc
     7861 gctgtcgaac tcgatagtgg gcggcgaccc ggtgacgccc gtcttcacgg cccgcgccgg
     7921 acagcccgtg cggttccgcg tccttcatcc gggcggccac gcccgcaaca acgtgttcca
     7981 ggtacacggt cacatctggg aggaggagcc ttacacaaac tcctcgaccg tgcttggcag
     8041 caacctgttt tccgagtgga agggttcgca atacggcgtc gggccgggca gccacttcga
     8101 catgctgctg aagaacggcg cgggaggtaa gttcgggata gtcggcgact acctgttccg
     8161 cgatcagggt tccttcccgt tcagcggcgg cctgtggggc atcttccgcg tgacgccgta
     8221 gcccttagtt caatccgtcg aggaaggccc gacgcttaac cggtaatgcg cctctcacgc
     8281 tcgacgtgag aggcgcatta ccgctttata gccgctttca gttgagtgag tattaagaca
     8341 gattcactca agtgaacgcc gctctaattg attagatcag gcgccaatgc ggagtctaaa
     8401 gttcttgata ttttgtcgat gttcagactc cgggccgaag cattgaatat ctcgcggtag
     8461 gttccgtccc tctcatagag gtcttcatgt gcgccgtgct cgacgacgcg gccctctttg
     8521 agcacgaaaa tgatgtcggc atcaataatt tgcgagatgc tgtgcgagat gatcaccgtg
     8581 gtgcgctctt ctttgatggc gtcgaggctg tttttaatct gctcggtggc caccgcgtcg
     8641 aggctcgcgg tcggctcgtc cagaaaaatg acgggtgggt ctttcaggaa aagtcgcgcg
     8701 atggcgatgc gttgctgctg cccgccggag agcaggtgcg cctcggattc gtacttgttc
     8761 ggaagatgct cgacctgctc gtgcaggtaa gccttgcgcg cggcctccac gacctccgaa
     8821 aacggggcgt cgatcttgcc gtagcgaatg ttttcttcaa tcgtgccctt gaagatgtga
     8881 ttcttttgta gcaccagccc gatttttcga cgaagcaggt cggtctcgta ctggtcaagc
     8941 tctttgccgt cgagcagaat ccgtccgctc gtgggcgtgt agaacctcgt caggagattc
     9001 aggagcgtgg acttccccgc gccggaaagg ccgacgaacg cgaccgtttt gccgtgcggg
     9061 attttcatgt tgacgtggtg gagagcctgc gtgccgttcg ggtaagtgaa gctgacatcg
     9121 atcaactcga aagtgccttc gaggtgcgac acgtcgagtt cgccgctcgc ctcgatttgg
     9181 tcgtcggcgt cgaggatttg aaagtagcct tcggcgtaag tcagagcgtc gttcatctcg
     9241 tcgtagattc tgtgcaactg cctgatgggc gcggagacgt tgccgaacag catgatgtga
     9301 aacatgatgg cgccgatgga catccacccc ccgaggacga ggtaggccgt caaaatgata
     9361 atcagcacga cgcccatctg ttcgacgaaa gtcttcaacc cgtcgaagac gaagttggtc
     9421 tgtcgttgtt tcaactgcgc gcccaccaga ttttgctgca accgaacctg cttctctttt
     9481 tcgtactcct cgcgcacgaa cgatttgatg acgatgatcg actcgatgat gttatggagt
     9541 ccgttgttct tctcctcgcg cagtgagcgc aagcctctgc gcgccccttt gagcatcgaa
     9601 gcctgtctct gactcagcaa aaaatagacc ggcaggatcg ccgaggcgac caggccgacg
     9661 tagacgttgg cgaggaacat gatgatgagc gccagaatcg cgttggcgaa caggggtaga
     9721 atatcgatga aggcgctctg cacgagcttg gtcagactct ccgcgccgcg gtcgatacgc
     9781 gtctgaagga tgccggtttg attgtccgtg gcgctgtaaa acgcgagccg gtaggtgagg
     9841 attttttcca cggcgaattg tgaaaggcgg ctcgacacgt tgacgcgaat tttctcgccg
     9901 aacagattct gcccgtacct caatcccgtg ttgatgattt ctttgccgag aagcacggcg
     9961 ctaatcagca agaggagaga agtcccctgt gaggccgtcc ggccttccct taaaagccgc
    10021 tcgatggtgt ccaccgtgta gcgcagaact atggggttga cctgcgccgc aaaggccccc
    10081 aaaagagtca gcgccagtga aaggaaaacc caacggctgt atggcctgac gaagggtact
    10141 aatcgccgga tgatttgcca gatgctcatt cgcaggtgcc gggaattaaa ctcatgttgg
    10201 ccgcattgat ttaagctcgc tgaagtttat acaatttccc cggctctccg catcgcggct
    10261 gaacggacgc gcgtccgttt cgggatggta aactgttact catcatgcac gcatttaccc
    10321 gcaggacacc gctttccctg atcggcgtgg tggccttcgc ctgcctggtc gcttccgcct
    10381 gctccggtcg cgcttccggt gagcttcgcg gccggggcgc gcagcagaaa gggttcgaag
    10441 ggttgtaccg catccgtaaa gacggaaaga ccgggttcat cgaccgcgcg ggcaaggttg
    10501 tcatcccgcc gcagtccgat ctcaaagtcc ccgactacgg gacgtacccg gtgttcacgg
    10561 aagggctggc gcagatcgaa gtcgagacgg acgagccggg gccgggcacg ttcagcaata
    10621 agaagaagat cggcttcata gatacgacgg gaaaggtcgt catcgagccg cgaatacttc
    10681 aaggcgcata cgacttctcc gagggcgtcg cggcggtgag tctgaattac cattacggct
    10741 tcattgataa aacgggccgg gtggtgatcc cgattgagtg gcagagtgcc cgcagctttg
    10801 ccgagggctt agccgcggtc ggcagagacg cccagttcgg cttcatcgac aaggcgggca
    10861 aggccgtcat ccctttacag ttcgacggcg cacagagttt ctcagagggg ctggcggcgg
    10921 tgatcgtcaa ccgcggaaag gtcttcatcg ataagaccgg gcgggtcgtt cttgagccgc
    10981 aggttgacga cgcacagagc ttctccgagg ggatggcgct catctccgtc aacggcaaaa
    11041 gaggcttcat cgataagacg ggtcaggtcg tcatccagcc catctacgcc gtcgcgcaag
    11101 acttttccga cgggatggcg ctcgtcaggg ttgacggcaa agtcggctac atcgacaaga
    11161 ccggcgcggt ggtcatcaag ccgcaatacg aactcgcctt cgacttctcc gaagggttgg
    11221 ctcccgtcaa gattggcgac agttggggct acatcgacaa gacgggtcgg gtcgtcatcg
    11281 agccgcgatt ctacagcgcc tatgagttcc gcggcggttt ggcgtccgtc acgacgagcg
    11341 aaggggacgg ctacattgac aaggccggca agtacatctg gccgccttcg aagtaacgac
    11401 gaggcgggcc tctcgtttca agaccgaaga aaggcagtag acatgagaag aatccgacgg
    11461 ccatgcgtta tcaccgcagt tgcgctgctg gcgctggtca gccccccgaa atcaacaaga
    11521 gaatgcggcc gcatctgaag atgagcggca cttcataccg cctcgacgag acataagtga
    11581 aggtcggcat ggagtgaaaa tacttctacc gcgccgtcga ttccacggga tgcacaacct
    11641 gacgaaagac ttagcaacgc cccctcttac cgcaaagtaa ttcctgcaac acaaccatgc
    11701 tccgcgcatc accggcgtgc cgctcgccat cttttagtcg aagctcctgc catgcgcgct
    11761 gccgccggcg gtagcggcag tcggcagtgg cgcgtccgtg agcatgtgct tgtcatgcgc
    11821 ccttcagctc tcgatcttca cctcctcgac attatcaaac ctctccagat cgatcagcag
    11881 gtgatagggg tcgatgccgg cgtcggacgg ctcgcggggc accgtcaccg tgatcgtctg
    11941 ctcgccggag cggatgcggt gcatttgcag gtagagcgtc tcgccgaagt ccgcgccctg
    12001 cttctcggtc ggggcgaaga ccccgatggg cacccactcg tccatcggcg cctcggtctc
    12061 gacgcccgcc ggatcgacgg tcaccttgcg cgcccgcacg cggagcgtca cctgccaggc
    12121 gcccgcatcg gtctgctcgg ccgtggctcg ctccgtctcg agttcccaga aggtgttcgc
    12181 ctcgaagagg tcgtgcagca agtaccggag cgagtccggc gtgaccgcct gaagctcccg
    12241 gtagagatcg agagaggtcg ctaggggcgg cgctcccaaa cgatgcttct cgatcaagcg
    12301 ccggaaggcc aggtttaccc gctcctcgcc catgtactcg ctcagggcga acagcgcgaa
    12361 gggacccttg cggtaggccg catacgggtc catggcccga agcagcggga cggactggcg
    12421 gatgggcggg attggagacg gctgccggaa gaacctccga agccgctgca ggtgctcgcg
    12481 cccgtaggtt tcttccacga cccccatcgc ggcgtaccac gcgaagcttt cggacagcaa
    12541 gggggctccc tcggcaaagg cgtatgggac tccccactgg tgcgccatct cgtgcgccat
    12601 gaccgcgaac gggaggtcga ggccccgcga atcatccgcc ggattgaagc gggagaatcc
    12661 ttccgtgtaa tcgattgtgg tcgcgtcggc atgcgcgcct atcccacgac cgggattctc
    12721 gatgagcctg atgtagccgt aggggtaagg accgaacgcg cgggtgtagt agtcgagcga
    12781 ggcgcgtacg ctgcggagca tgcggttcag gttcgcggcg tgcctggggt gatggtagac
    12841 ctggatcgcg acgacccgcc ctgagcctga cgaagggtca ttccactgtt cttcgtgcac
    12901 cgcgtaacgg gcggagaaga agcggtgctc gttcccaatc ggggtatcgg tagcatagtg
    12961 gaagtagcgg cggccgcctt ccgtccatgt ccggcgcagc gcgcccggcg cgacggccgt
    13021 ctgatccccg tccgtgccca cgactgcttc aaaggcgatg cgctcggcgc cggtcagatc
    13081 gtcgcccgct tcgccggtga tgtcctggcc cgcttccgcg tcggcgagag aagggaacag
    13141 ggggcgcgga gcaagcccgt gcgcccgccg ttcaccgggt ttaaagagct cgcgacgggg
    13201 ctgatagccg atggcgggca gccaatcgtt cttgaagtag gtgctgttgg ccgccacgga
    13261 ggcgccgccg ccgctgttac ggaagccctt cggtttgaaa tgcacctcga agctgagccg
    13321 cagcgagtcg ccgggctgga gcggctcctc caggacgtag atgcggtggc cgagattctc
    13381 gtccgcgagc acgcgcgcaa ccggccggtc aaaggcgacc gctccggtct cgactcccga
    13441 gtcggtggcc acgtggatcg agtcgattgc cgctgcggcg ccgtttacga gttggtatgc
    13501 gccgcgaatc tccacctccc gccgccgggg ataaatctcg acgtgcaggt tggtccccgt
    13561 cagccggggt tgcgggacat tcgcgtactg cccgaagcgc cgctcatact cggcgcgccg
    13621 ctccatcttg ccggaggcgg tagtgtattc gttcagcacg ttcgtgttgt agaagatgaa
    13681 cccgcccagc gtgaggacga gcccgaccgc cgccgcggcc gccccggccg tcggacgcgt
    13741 gaagcgacgg cgcgccagtt ggagccgcac ccgaaggccc ccctccatgc cccgcaccca
    13801 gagcagcctc gccacgaccg cgagcagcag cgcccacgcg gcccagtaga gcatgaacca
    13861 cagccacggc ccgagggacg cgccgaatcc gcgcatgtcc gtgtacgacc agtctggagc
    13921 ggacccgtag acgagcaggt tgtgctcgat tccgaggctg gaggcgaaga ggatggacgc
    13981 gtaggcgatg agcgccacca ggtggccgac gtacttctga ttgaccagcc cctgcaccac
    14041 gagggcgagc agggcgaaga ggaggtactc ggggagttga agcccgaaga ggacgcgcag
    14101 gtacagcccc gtctcgaaat cgtcataccc catgcgcgtc tggacgagca ctccagccat
    14161 ggtcaggagc gccatccacg cgacgaggat gagacctagc ccgaggaact tgcccaggaa
    14221 ggagacccat tccggcaccg gcgccgcgtc ggtgatctcg cccaggccgg ctttccgttc
    14281 ccgccagacc agctcgccgg cgtagaagac gatgagcagg ggggtgatta cccagggggt
    14341 gaggggattg gtcaggcggg ccgtcagaaa cgtgaggaca tactcggttc tgggcagcag
    14401 cggagtgccc atgttggaca tgttctcggg catgaccaca accgcgagcg ccgcgatggc
    14461 ggccaggagg acgagcccgc cccggctctt ggcaatcgtc cgaaacgagt cccaggcgat
    14521 tgccagcgtc tggcgcagtt gggtcgccga gccgaacgtc tgccggaact gcggcatcga
    14581 gatcatgctc cgcgcgatgt cgttaagcgg aggcgtcggg gcgtgcgcct ctcgtcgtcg
    14641 cgtgaggcga ctccacctgt ggctcgctgt cgggtgggcg aagcgaaagc gcaagtaggt
    14701 gaacgcgagc gtccccagag cgatgccgaa ccacacgagg cggctccaga gcagcgggcc
    14761 ttccagctcg atcagacgcg tactcttctc gatgggcgtc catccgagga tcagatcgga
    14821 cgtgatgaat acgtggccga agacgtcgag cagcgccgcc aggtcctgtc gctccaggaa
    14881 aatcgccgcg aaaaacatcc cgccgtaggc cacgaagaag aggagcacac tgccgacgta
    14941 gctcgcgatg gcccgacggc tgagcgccgc ccacgcaaac tggatcgccg aacccacgaa
    15001 ggcgttcggc aacgcgatga agccgtaggc cgtgaggtag gcagccggcc ggaacggacc
    15061 gacgacctcg gcctccacgc ccggcgagta gacggcgagc atgacgccca ccggcacggc
    15121 gagcaggatc agcgcgttga ggacgaagcc ggcgaggaat cgtcccccca ggtactcggc
    15181 cttgctgacg ggggcggtgt aggtgagggg atgcatcccc gtctccacgt cccgcgccgc
    15241 cgtctcgccg gctacgcccg cggccaccac gaaccagaac aggccggcga acaccgtgac
    15301 gctcgcgatg acgaagggcg agttgagata gaagtcggcg taaagcgcgt cggcgaggtg
    15361 gtttcctctg acgaacagga aagcgacgac caccagggcc gcgaaataga gccaggtctg
    15421 gagacggcgt acttgatagg cgaactcgaa gcgaaagatc tcccggaact tcatggcgcc
    15481 atctccggct ccggctgctc gcggcgccga ccgatgtgac ccgccatcgt gctgaagtag
    15541 acatctttga ggtccggctc ggcgggctcg aagccggggt cgggcgccgc atcgctgaag
    15601 acgtgtacga cggtgcgtcc tgcgaggagc ttcgtcgaga tcaccgcgtg cgctcgctcc
    15661 acctctgcca gcgcaccctt ctcgatcacg ctgcgccaga tcctccccct catctcctca
    15721 atcgcaccga gcggctcggc ctccagcaga atctcgcccc ggtcgatgat cgccatccgc
    15781 gtgcacagct cggagacgtc ctcgacgatg tgcgtcgaga gaatcaccgc gctgttctcg
    15841 ccaagctcgc tgagcaggtt caggaaacgg acgcgctcgg ccgggtcgag gccggccgtc
    15901 ggctcatcga cgatgagcag cttcgggttg cccagcagcg ccacggcgac gccgaaccgc
    15961 tgccgcatgc cgcccgagta gccgccgagc ttctgcctgc gcacctccca caggttcgtc
    16021 tgccggagca gcgcctcgac gacctctttg cgcgccttct tgtcggggat gcccttcagg
    16081 atggcgaagt ggtcgagcag ggcctccgcg ctcaccttcg gataaacgcc gaactcctgc
    16141 ggcaggtagc cgagcgtctg ccgcaccgcg tccttctgct gcacgacgtc gaggtcgccc
    16201 aggtgaatgg cgccggtgtc cggctcctgt agcgtggcca ggatgcgcat cagcgtagac
    16261 ttgccggcgc cgttcgggcc gagcaggccg tacatcccca ccggaatcgt gagcgtcacg
    16321 tccctgagcg cctgcacgcc gttagggtag gtcttggata cgtcgcgaat gtgtaattcc
    16381 atcaggaaat gattgttgtt tggaaagtac tttgcaatct aaagcaacca aatgctcaag
    16441 tcaaccttta aatagttggt ggaggcgaag ggttcacgaa ggaactgctt gtgaacctac
    16501 ctggcgagtt gtggaaccgc acacggagaa gtaaagacgc acccgtctgt ctgtgagctt
    16561 ctcataactc ccgaactccc ccgaggcccg ctctctaaac accggccggc gacccgcggt
    16621 cgaacttatc aacctcgccc cagtaccgag gttgaaccag cagccggcgc accagcgggc
    16681 ggagaccggg cacctcaagg tgcgagtacc cgcccaccag tgcggagaag acggcccgcc
    16741 gccgcaggcg cagcaggcgg cgcacgaggt cgggcgccag cagcgcctgt accccgagca
    16801 gtatctggta gcgccaagcg ccgaggtgac gccgatactg ctcgtagagg agggccgtgt
    16861 gtctgctgaa agccaggtcg cgttccaggt gggcctgcct gtcgccccgc cactgctcgt
    16921 agccttccgg caaccctccg atgtttaacc cctcgccgac ccgcctgaag acggcgtaaa
    16981 gctcctcgcg ctcggcggcc ctgagcggcc cgtagagcag gcggtgcgcg cgctccgagt
    17041 agtcaacgag catgtagagc acgtccctaa acgcccagtc aggaatcctc tgcccacgct
    17101 ggctctcgac cgcgccgtgg atcgcgttga tgcgcccgag cgtgcgccgc gccgtgtcct
    17161 catccgcgaa cacgatctcc tgcgcgtagc ggacggtcga gaagagtcgc ccgacggggt
    17221 cgttcggtat tccgccggtg aagaagagcc aatcgaccgc gcggtttaag gcgaactcgg
    17281 cggccgagcc ggcgaagacg agcaggacca gctcggggtc gccccagatg ttcctgacga
    17341 ccgaaccggg cgcgacgaaa tcgcgcgtca cccgtggggc cgaaagcccg acagcatcag
    17401 tgccgacggt gtgagccact ctttgaattt gccttttcct tcttcgtcga cagagaggga
    17461 atagttagcc gcaggtagtt cgagcgtact agcgaaaata tggagctaca tttcctgcga
    17521 tagttttgat ctcttttgtc gaaagtttca gttggcgaaa cgcttcccag gattcgcgcg
    17581 gccgaaggtt gtttccgagt gccgaatctg agccgtagag tatccgtttg acaccgattt
    17641 ggcgaatcaa tttgactaac agttcggaga cttccgccga gtttgtcgga tgggcgtttg
    17701 tcgccacatc aaaccagagc tttcgtgttc gagaatcctt tttcgcaacc gcatcagcca
    17761 gcacttcaat gacagaatgc gccggagggt catcgtaacc cggacccgag ctggcaagat
    17821 gagcgatctg caccggaata tcgggggcga aagaaagcaa ttccagaaaa gcgcgtgctt
    17881 gttcaggccc gtaaggtcgc tttttggaga tcgacgctct catatgcacg atgatcgcca
    17941 tgcgctgaga atttgccgct tgaaaaactt tctttaattt ttcgaaatgc tcagggactt
    18001 ccgtctgcac atccgaactg ccgaaatgca gtttgatacc gtggcggagg ttcgcatctt
    18061 tagcacaacg cgcaagctca tcgagggcat attctttgag cgggttgaag ctgcaaaagg
    18121 caatcagcct tttgggaaag agagcggctt gggcggcatt ccaatcattt tccgctttga
    18181 ccttggcgta ttcatccggc gggtccgagc cgggtcttcc atatccaaat gctgttgata
    18241 aaagcacggc tcgccggatt ccggcttggt ccagcaatcc gatgatatct tgagccgtaa
    18301 tcgtttttaa acctgagggt tgaaaatcaa cgcgagcttg actgaacaaa tgctggtgat
    18361 gatccgccgc gggaacgatt tgctcgggtt gagcatttga tcgagaggac tgtccgtttt
    18421 gggcaattgc tatggcagag gccagggcaa tcaatgaaaa agtctgaaaa actaaaaata
    18481 atcttttcac cataaaataa cctccggaat cgtttacaac tagaaaacac cgcgagcgga
    18541 tggtgtgtga caaaactact cgctttaaat ctaaagttcc gcaaactgtt gtttcataac
    18601 tgaatccgag tcatgagttg aaaaagagag gccgtcgtcc ttgagacggt gtgtagaatg
    18661 aagttgcgaa acgacttcta ccaccaaagg acgacgacct atggccgaga gcatagagca
    18721 tcagatgacg gaaatctact gcttcgttga cgattacctt gtggctcatc cggcgctctt
    18781 gcgctggcgg cgttcgcccc atgcttgccc gcgcttcacc gactcggaag ttatcactat
    18841 cgctctgttg caaggcccgc tgggcgtcgc ctcgttgaag aaggcttatc ggatggtggc
    18901 gaagaactgg ggggcggctt tccctgcctt gccgacctac acacagtgga tgcatcgtct
    18961 ccatcaactc accaggcagg tcggggcact gcttgaggcc acctgcggcc acgacgcggc
    19021 gcaggctcgc ctctacctga tggactccaa acccatcccg ctgtgccacg tcttgcgaca
    19081 cgggcgcgtg cgccttctgc gcgaggacgg cgcacgcttc gggaaaacca gtaagggctg
    19141 gttcttcggc ttcaaactcc acgccgtgcg ccatatcgcg ggccgcgtga tgaacatcat
    19201 cctgacgccg gcgaactggg acgaccgcga cccggcgccg gcgctctcgg aaggcaccga
    19261 cggcggcatt gccattgctg acttgggcta tcgcggcccg aagtgcgccg cgagtctgga
    19321 aggggccgag gggcttctga tcacgcgggc cgacgctcca gagcgcaagt tcctgctctc
    19381 aagtcaggca aggcgtcgag acgatgttca gtcagtggtg gcatcagttc attgatcgcg
    19441 tcttcagccg ctcgtggaac gggttgtgga atacgctcaa gctcaaggtg ctgcattaca
    19501 acttggttca tgctggcgtc gtctcagcct gattccaact catgactcgg attgtgttag
    19561 ccgcgcctgt acgtgctgaa aaccaagcgg gggtgcgcat gactgactct ggaatcgccc
    19621 accctccata cctccacacg tttgtcgcct tcgacatact ccattaaagc cggcgagcgc
    19681 atgacagccc tcttacacgc ttatcaccgc gcgataaggt gaccggcggc gacagccagc
    19741 actgtcatca ctccatagcc gagttcgcgt atcccgatct gcttggcgct cgcgagttgt
    19801 gggtttcggg acaggccgat cacggcgcgc agaaacagaa ctgatagtgc cgccaccgcg
    19861 agaatgggaa ggagcctcat gaatacaagc gcggcgacgg ccatcagggc gcaaacgtgc
    19921 gcgatgatca cgggagaccg gccgggggtt tcgccgcgct tgagactcag gcgtgcgcgg
    19981 acgtacagga tcgtcgggac gacgcgcgcc gccagaaccg cccacagccc cgcggccgca
    20041 gggagcgacc agccatcggc cagcgcgatg ctcgccgaga cagcggccag gccgagcgac
    20101 ccggcaacct ccggcgcgag cgcgcgactc cggcccgtcg cgtcgaactt gagttgtacc
    20161 gccgcgaagg gcgctgccag tagcaggggg aggaaaaatt gatggctgac ggcgctcacc
    20221 gatgcggcca gcagtcccgc caccgcgacg gcggcgtaga gcagtgtgaa gcgttcggcg
    20281 accgccgtgc gcgggaagcg tcgcccgcga cggcgatctc ccgccacgat cttgaagggg
    20341 tggcgcgcga gaaacgcacc catcgtggcg acggcgatga agagtcccgg aaccgacggc
    20401 gcgaccagaa gcccgagcac gaccggctca agcataagcc cccacccgcc gtgctccgtc
    20461 ggcagtgcga cggtgcggac acggacgcgc gcctgcgccg cgccgttctt gcccgtaatg
    20521 ttctttgggg gttgcccctc gaataaaatc gtccgactca tcttgttctt cccgacttga
    20581 aaaaccgtat gccgcctaaa accgtttaga aagctcagcg tagaaaaacc tcgccgcggg
    20641 gttggcgccg cccgccgggt ggatggatga ctgcacggcg ccgccgcgca cgccggcgcc
    20701 gtagagggtg agcgtcgtgg tgggcgtgac cgtcacgtcg gcgcttaagt cgaacagcgt
    20761 gcccagactt ttgcgcccgc cgctcgaacg gccgacgtag ccgaagctgg actcctggaa
    20821 cgcgccgccg ccgacgtacc agaggtcttt gcggttgctg agtctgaggt gatgcacttc
    20881 ggcgcgcagc gcgaggcggg cgtgcggcct gagcttcagt tgggcgtaca cgtcctcgtt
    20941 gttcatcatg ttgtagaaag ggaagcgcgc gtaccacctc ggcgtcggca tgacttggaa
    21001 gaaggtcgtg tgacgagcgt ccgtcgggtc gccgtcgccg gtcgagcggg agtagcccgc
    21061 gcgcagccac ggcctcatgc gcgcggcaaa cttagccgcg ggctgcaagc cgccctcgag
    21121 gacgagcgcg cccgcacgat gtccctgatt gccccagcgg ccgaactggc cgacgcccca
    21181 cgcgagcaag tccacagtgc cgcgcgcggt cttgacggcg gcgacgtagt taccgccgac
    21241 ggtcgtcagg cgaatgttct ccgcgtcggc gcggcggagc gcgggcgcgc ggttgtcggt
    21301 cttgagcgcc ccgcgcccgt cgtgatagtg cagggccagc acgcgcaact cgctcgtgac
    21361 ccgctcgccc gcgagaggct tggtgtacgc gccgtaccag aagtccacgt ccagttcctt
    21421 cacggcgcgg agctggaaga caccctcggt ggggcgtccg ccgacgaagg tgaagtttcc
    21481 cgacttggtg ttgcgcacgt actggacggc gtcgaagctg cggcccacgt gcgagaagcc
    21541 gaagtggccg atgaggcggt gggcgacgtg gtcgcgcttg agcgccgcga gcgtcgcgtc
    21601 cgacggcgtc gtctccatgc cgtcaacgaa ctcgaagcgc ccaacgcgca aggaactcgc
    21661 cttgtcgccg aacaacccct tgacgcgcac gaatgcttgc ttgagcacgg cgctcgcatc
    21721 ctgtctgccg ttggccgcga agtatgagcc gccgaggcct aactgtccct gcggcgcggg
    21781 cgcgaccgag cgctcgggca cgttcatcag gacggggaat tcgccctcga ccagccagtc
    21841 gaacttctcc ttctgctgac cgacgctcag gcggagcacc gcggccccga aggtgtactc
    21901 gtcctcgtag cccggcgtct tgaaccagcc gtagttctcg acgcgcaggc gcaaggaacc
    21961 cgaaaggttc acgccgccga tcttgagttg cttcggcgct ggcggggcgg cggcgggctt
    22021 cgatgtcgtc gctgacggcg gcgcgagcgt ccgtgtcttc tcgtcgccgg aagcgttcat
    22081 tttattgtcg gcgagcgccg gctcctgcgc gcgggccgac tgaatcccgg gcgcggcgca
    22141 gagcaagagc agcgcgcaga aaaacgtcct gagctttggc gagttcatgc gttcaggcgc
    22201 ccctcatgtt catggcgcgg gcgctcaaag acggggtctc ttccttcctc gtgccgttgc
    22261 gtggcttgag gaagacgaag tagttgacgg cgaggcagag gagcgcgaag gccgcgagca
    22321 ggatgaagcc gggccagtag acgccgaacg agtcgcggat gacgccgagg acgagcggcg
    22381 ggaagaaacc gcccaggccg ccgaacgcgc cgacgaggcc cgtcaccgtg cctgtctcct
    22441 tcgggaagtg ctggggcacg agtttgaaga ccgccccgtt accaagaccc agagcggcgg
    22501 ccgcgccgag cgcgccgacg gagaaggtga cgaaccacgg gcagcccatc atcgccccga
    22561 aaaaggccac cagcgcgaac acgccgagga ggacgcgcgc gccgccgaag cggtcggcga
    22621 gcagtccgcc gacgggccgc aacagggtcg ccagcaggac gaatccggcc gtgcgcgcgc
    22681 ccgcgtcggt cggcgtcagc ccgtactggt cgcgcagcaa tgtaggcagg taaatcgaga
    22741 acgcgacgaa gcctccgaag gtcacgaagt agaagagcga cagcacccac gccagcggcg
    22801 agcggcgcag cacgttcaag ttttccgcga gcgtcttcgg cttcgtcgtg acggccgcgt
    22861 cgcgcgcgaa caggtagaag accacgcccc agacgagcgc ggcggcggcg aagatgtaga
    22921 agaccgtgcg ccagtcgccg agcgcgagcg cgagcaccgg cgcgccgaag acggcgattg
    22981 actgcccgat gttgcccatg ccgaagacgc cgagcgccgc cccctgcttt tcgggtgcga
    23041 accacttcga cgtgaagccg acgcccaccg aaaaggtcgt ccccgccatc cccaggaaga
    23101 ggccgaggag gagcagttgc gcgtagctcg tcgagaaggc gatgcctacg gccgggagcg
    23161 ccgagagcac gagcagggcg gcgaacacgc gccggccgcc gaagcggtcg gccagcatcc
    23221 ccgccggcag acggcccagc gagccgagca gcacggggac ggcgatcatc aggctcttgg
    23281 ccttcgccga caggtcgtac atctgcgtga acgtcggcgc gagcgcggcg accagccccc
    23341 agacggcgaa ggagacggcg aaggccgtcg tcgaaaggaa gagggcgcgc gccgcgcccg
    23401 gcttgaacgt ctcactcatc aatcattact ccacgagatg aaaaaataac tcaaagacga
    23461 aagataacaa ctcgcgcggg cagagccgcc ctgctgtcta atggcaacgg gcgtgccttg
    23521 cgaaagcatt gaaataaggg ggaaattgga cagctgggtg agtatttcta cgcgcgcagg
    23581 gctgataacc ggggaatttt ccgactctta gtcttcgccg cgtgcctctt cgtcgcagcg
    23641 tttacagtcg agttcgaggc cgaggcgtga ggcgcggcct tcctcggtga gcacccacgg
    23701 gcggctcgtg agcggcgggt cgtggcgcac gtgctgggga tggccgcact caagctcggc
    23761 gcgccagtgc ccctcgtcgt ccgtgtggaa tcctgcgatg cgtcgtttca tcccggtggc
    23821 gcggccgtcg cgcggctcac ttacgctgcg gcgcgctctc gcggacgtag ccggtgctcg
    23881 tgtagcgccg ggagacgggg cgccaccaca gcactatcga gagcgcgatc agccagcaga
    23941 cgcccgaggc gacgacgagc gggacgagta gagagtagtg gcgcgccagc gccgcctcga
    24001 ccgccgccga gagaatccag agctgcgcga cgttaatcat cacgagcagg atgagacgcg
    24061 cctgaaacac cgcggcgtgc ccccggcccg cgccgcgccg actcacctgc acctgtgtcg
    24121 ccctctcaga tggcatgttc gccgcccccc ggcactcggc ccgtcgccca cacctcgccg
    24181 ccgtcgcgca cctccaactc gatccggtcg agcgggcgtg gcggcgggcc gtagagaacg
    24241 ttcccggtct tgatgtcgaa cgcgccctcg tggcaggggc actcgaagcg agcgtgcgcg
    24301 cgctcgtagt agaccgggca ggcgaggtgc gtgcactttt gcccgtaggc gtggtactgc
    24361 ccttccgcgt cccggacgag aatcgccgtg tccttctcgg atgggtagag gaaattcatc
    24421 gccgagccgg gcgcgagact ttcggcgccc tcgacgcgcg acggggcgaa ggacacggac
    24481 gcgcgcgcgt cgagcgcggc tttggcggcg aacccggcgg cgctgacgaa gagcgccgtc
    24541 gaggtcaggg cgaggaagtt gcagaactcg cgccgcgtca cctgcgcctc ttcgcctcgc
    24601 ccgtagggga agtcgtgctg ggagcgggcc gtcgcctcgc gcacgtcgcg ctcgtcggag
    24661 acgccgcccc cgtcgtcagt catcacgcgc tttatcgtct cgttaagatt cttcatgtcc
    24721 gtcttcaagc tgttagccat tagccgaaga caaaaaagct gaccgctaac agctaatagc
    24781 taacagctcc ccctagaatt cattgctctt gtcactctcc tcgacctgcg tgtcgatcca
    24841 cgacccggcg gtcggcgtgt ggccgccttc ggcgagactc aactgtcctt cgaagacccc
    24901 gcagccgtca acgtcaacct gcttggcgtc ggtcgggagc atcaggaaaa ctttcgtctc
    24961 gaccttctgg ttgccgaaga cgtggacgtt gaccggcttc gtccgccgca gcgggacgat
    25021 ctgctcgtac cgtccgaagc tgatggcgcc cgtcgggcac acgctcgcgc acatgggttt
    25081 gagtccttcg gaggtgcggt cgaagcacat gtcgcacttc atctggaggt gcatcgccga
    25141 gttgtacttg ggcacgccga acgggcaggc gttgacgcag ttgcggcagt cgagacaccg
    25201 gggcttcagt gccgacatca ccacgccctc ctccgacacc ttgatggcgt cggccgggca
    25261 gacgagcgcg cacgtcggct cgttgcagtg catgcagacg gtcggcatcg tggcggtcgt
    25321 ctcggcgcgg tcgaggtagt caacgtagat catcgagtac cccttgtgcg tcccgcactc
    25381 gcggcacgcc gccacgcacg agcggcagcc gatgcaccgc tgcgggtcga tgaacatcgt
    25441 ctcttccata aaaagctgtt agccgttagc cgttagctaa aaccgtgaga cggccaaaac
    25501 ctccgttaag atttgaactg cgaccgtccg ccgtgggaca aaagctggaa gctgacagct
    25561 aatagctcat ggctattagc taacagctct cacctcactt cgctccgtga cgtgcccttg
    25621 cgccgctcgc cgaggatggg gagacggcgc gagtgaatct tctcgatgcg tgcggcgcac
    25681 gccttgtact cggggatttt tgaagtgggg tcgagcgccg ggttggtcag ttgattcgcc
    25741 gcgagttctt cgccccagtg gtaggggatg aagatggtgt cgggccgaat cgtgcggacg
    25801 acgagggcgg ggaagacgcc ctcgccgcgg cgcgagacga ccttaacgcg ctcgccgtcg
    25861 ttgaccttga gcctcgcggc cgtctccggg tgaatctcga cgtaaggctc cgggcactgc
    25921 tcgacgagga agccgatgcg tctggtctgg ttccccgaaa gatactggta gacgacgcgc
    25981 ccgctcgtca gaatgagcgg gtactgctcg tcgggcactt ccgccggcgg attatattcg
    26041 accggatgaa acttcgcgcg cccgtccggg tggtaaaact tttcctcaaa cagacgcggc
    26101 gtgccgggat gatcctccgt cgggcacggc cagaagacgc cgttctgccg ctcgatcttc
    26161 tcgtaagaga tgccgtagta gtcggcgttg ccgccgcgcg aggccacgcg taattcgtcg
    26221 aaaatctcgc gcgggctgtt gaaggagaaa tatttcccgc gtcccatccg gcgcgcgatc
    26281 tcctgaatga tccaccagtc gacgcgcgcc tcgccgggcg ggtcggcggc cttgttgatt
    26341 ttcaacacgc gcccctcgct gttcgccgtc acgccctcgt cctcgctcca ggtcgtgccg
    26401 ggcagcacca cgtcggcgta gcgcgcgccc tcagacatga agaagtcgat gcagacgtag
    26461 aagtctaagc cttcgagcga gcggcgcacc tcgttcgtgt ccggcaaaga gaccatcacg
    26521 ttgttgcaga tggagagcag gccccgtatc tcgccctcgc gcatcttctg gaacatctcg
    26581 acgacggaga cgccggcctg cggcaactcc
//
LOCUS       DASVES010000308         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1030221, whole genome shotgun sequence.
ACCESSION   DASVES010000308 DASVES010000000
VERSION     DASVES010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030221"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VF240_12505"
     CDS             complement(<1..541)
                     /locus_tag="VF240_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7176074.1"
                     /translation="MLRYIIFAPLAGAAVNWLVGRRVRDERLIGAVACLSVGVSAVVA
                     FLAAFGPEGALRGDVPHVVFDHLWTWISVGGFRADFGLAMDRLSGIYACFITFVGFLI
                     HLFAVGYMHGDKGFYRFFAYLNLFMFMMLTLVLADNLLLMFVGWEGVGLCSYLLIGYY
                     FDRKEAGDAAKKAFVANRVG"
     gene            complement(636..938)
                     /gene="nuoK"
                     /locus_tag="VF240_12510"
     CDS             complement(636..938)
                     /gene="nuoK"
                     /locus_tag="VF240_12510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX7176075.1"
                     /translation="MIPLTWYLALSAVLFTIGVVGVIIKRNAIQMFMCIELMLNAVNL
                     TFVAFSSYFGDLTGQLFVFIVMTVAAAEAAVGLGIIIAVFRNRESLDVDEASVLKY"
     gene            complement(946..1641)
                     /locus_tag="VF240_12515"
     CDS             complement(946..1641)
                     /locus_tag="VF240_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX7176076.1"
                     /translation="MLTVLLFILFGGLAVGCAIALVAQRNPLYSAISLVGVFVSLACL
                     YVMLAAPFIAAVQVIVYAGAIMVLVVFVIMLLNVEEEVRRPVRLFHLVPIAMGLAAVL
                     IGETAFILTLRGASGAPQAPNQQVGLTSSIGTGLFTEYLLPFEITSVLILMALVGAMT
                     LARTATVARPETPGDFRPDSGLKLVADAAGDGGGAVIQPGKTEETGRAMAELQIERGE
                     AAAPSSRPPRGEG"
     gene            complement(1669..2688)
                     /gene="nuoH"
                     /locus_tag="VF240_12520"
     CDS             complement(1669..2688)
                     /gene="nuoH"
                     /locus_tag="VF240_12520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX7176077.1"
                     /translation="MQLLGINIAFVVVLMIVAYTVLAERRVLGFIQGRLGPNRVGPGG
                     MLQPFADLLKFIFKEELIPDKATKFVYLLAPIIAITMALMPIIVYPFGPTINIPFVGP
                     VALVIARFDIALLYVLGVTSVGVYGIMLAGWSSNSKYSLMGGLRASAQMISYELSLGL
                     SLVGVVLMSGSLDLYEIVQQQSGWYGMKWNLWRLPAPQIIGFFVYLISAIAETNRVPF
                     DLPEAETELVAGFHTEYSALKFALFFMAEYVNMFTVSVLATTLFLGGWSGPFVEQAPI
                     LGIFYFLGKISFFLFLYIWLRGTLPRFRFDQLMNFGWTFLLPLAIINVVISATLAFFF
                     PEWLR"
     gene            complement(2834..>3446)
                     /locus_tag="VF240_12525"
     CDS             complement(2834..>3446)
                     /locus_tag="VF240_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7176078.1"
                     /translation="AKFDFVVVQELFHTATTEHADVVLPAASFAEVDGTYTNNDGMVQ
                     RVRQSVPPVHQSKPDWGITSQIADAMGVAFNFQMSASAVFREIAERVGPYEGLRYPLL
                     KDESNPRQAKHALAGKRDLSAELETLRSSVREMSAAGEKTQGTPPVGHELFKPGTLTG
                     KTPQFHLLAAGNPKPESVLVSPLYQITIDTGRQRRETAAATGD"
BASE COUNT          732 a         1100 c         1091 g          523 t
ORIGIN      
        1 cgccgacgcg gttagcgacg aaagccttct tggccgcgtc gccagcctcc ttgcggtcga
       61 agtagtagcc gatgagcagg taggagcaca gtccgacgcc ctcccagccg acgaacatca
      121 ggagcaggtt gtcggccagc accagcgtca gcatcatgaa catgaacagg ttcaggtagg
      181 cgaagaagcg atagaacccc ttgtcgccgt gcatgtagcc gacggcgaac agatgtatga
      241 ggaagccgac gaaggtgatg aagcaggcgt agatgcccga gagcctgtcc atcgccagcc
      301 cgaagtcggc gcggaagccg cccacggaaa tccacgtcca caggtggtcg aagacgacgt
      361 gcggcacgtc gccgcgcagc gccccttcgg gaccgaacgc cgccagaaaa gcgacgaccg
      421 ccgacacgcc caccgacagg cacgccaccg ccccgatgag cctctcgtcg cgcacgcgcc
      481 gccccaccag ccagttcaca gcggctcccg cgagcggggc gaagatgatg tagcgaagca
      541 taacagtagt gatgagtgat aagtgatgag tgatgagata agaagaagcg gcggccttct
      601 cttatcactt atcactcatc acttatcacc gcccttcagt atttcaaaac gctcgcctcg
      661 tccacgtcca gcgactcgcg gttgcggaag acggcgatga tgatgccgag tccgacggcg
      721 gcctcggcgg cggccacggt catcacgatg aagacgaaga gctggcccgt caggtcgccg
      781 aagtagctgg agaaggcgac gaaggtcagg ttcacggcgt tgagcatcag ctcgatgcac
      841 atgaacatct ggatggcgtt gcgcttgatg atgacgccga ccaccccgat ggtgaacagc
      901 acggccgaga gcgccagata ccacgtcaga ggaatcatcg cccgctcacc cctctcccct
      961 cggaggccgc gacgacggcg ccgccgcctc gcccctctcg atctgcaact cggccatcgc
     1021 gcgccccgtc tcctcggtct tgcccggttg gatgaccgcg ccgcctccgt cgccggcagc
     1081 gtccgcgacg agcttgagac cactgtcggg acggaagtcg ccgggcgtct cgggccgcgc
     1141 caccgtggcg gtgcgcgcca gcgtcatcgc cccgacgagc gccatcagga tgagcacgga
     1201 cgtgatctcg aacggcagaa gatactcggt gaagagtccc gtcccgatgg aactggtcag
     1261 accgacctgc tgattgggcg cctgcggggc gcccgacgcg ccgcgtagcg tcaggatgaa
     1321 cgccgtctcg ccgatgagca ccgcggcgag acccatggcg atgggcacga ggtggaagag
     1381 acgcacgggc cggcgcactt cctcctcgac gtttaagagc atgatgacga agacgacgag
     1441 caccatgatg gcgccggcgt agacgatcac ctgcacggcg gcgatgaagg gcgcggccag
     1501 catcacgtag aggcaggcca gcgagacaaa gacgccgacg agcgagatgg cgctgtagag
     1561 cgggttgcgc tgcgccacca gcgcgatggc gcaaccgacg gccagcccgc cgaagagtat
     1621 gaagagaaga accgtcagca taagccttgc gaggcgcgcg cgcccgtgtc agcggagcca
     1681 ttcggggaag aaaaatgcga gcgtcgccga gatgaccacg ttgatgatcg ccaggggcag
     1741 caggaacgtc cagccgaagt tcatcaactg gtcgaagcgg aagcgcggca acgtcccgcg
     1801 cagccagatg tagaggaaca ggaagaagga aatcttcccc agaaaataga agatgccgag
     1861 gatgggcgcc tgctcgacga aggggccgct ccagccgccg aggaaaagcg tcgtcgccaa
     1921 aaccgacacc gtgaacatgt tgacgtactc ggccatgaag aagagcgcga acttcagcgc
     1981 cgagtactcc gtgtgaaagc ccgcgacgag ctccgtctcc gcctccggca aatcgaacgg
     2041 cacgcggttc gtctccgcga tggctgaaat caaatacacg aagaagccga taatctgcgg
     2101 ggccggcagc cgccacaaat tccacttcat cccgtaccac ccggactgct gctgcacgat
     2161 ctcgtagagg tcgagcgagc cggacatcag caccacgccg acgagcgaca agcccagact
     2221 caactcgtag gagatcatct gcgccgacgc gcgcagtccg cccatcagac tgtacttgct
     2281 gttagacgac cagccagcga gcatgatgcc gtagacgccg accgaggtga cgccgagcac
     2341 gtagagaagc gcgatgtcga agcgcgcgat gacgagcgcg accgggccga cgaacgggat
     2401 gttgatcgtc ggcccgaacg ggtagacgat gatcggcatg agcgccatcg tgatggcgat
     2461 gatgggcgcg agcaggtaga cgaacttcgt cgccttgtcc gggatcagct cctccttgaa
     2521 gataaacttc aggaggtcgg cgaacggctg caacatcccg cccggcccca cgcggttcgg
     2581 ccccagccgc ccctgaatga agccgagcac gcgccgctcg gccagcaccg tgtaggctac
     2641 gatcatcaac accacgacga acgcgatgtt gatgcccagc agttgcatca acccacggct
     2701 cacgtcagcc ccgacaagat tgtccagcca ttgcataagt gatgagtgat aagtgatgag
     2761 tgatgagaaa gagataaggg gcggccgacc gccttttctt atcacttatc actcatcact
     2821 tatcactacc ttttcagtct cctgtcgccg ccgccgtctc gcggcgctgt cgtcccgtgt
     2881 caatcgtgat ctggtagagc ggcgagacga gcacgctctc gggcttgggg ttgccggcgg
     2941 cgagcaggtg aaactgcggc gtcttgcccg tcagcgtccc cggcttgaac aactcgtgcc
     3001 cgacgggcgg cgtcccctgg gtcttctcac ccgccgcgct catctcgcgc acgctcgaac
     3061 gcagcgtctc aagctcggcg gacaggtcgc gcttgccggc gagcgcgtgt ttcgcctgcc
     3121 gagggttgga ctcgtccttg agcagcgggt agcgcagtcc ctcgtaaggc ccgacgcgct
     3181 cggcgatctc gcggaagacg gcgctggccg acatctggaa gttgaaggcg acgcccatcg
     3241 cgtcggcgat ctgcgaggtg atgccccagt ccggcttcga ctggtggacg ggcggaacgg
     3301 actgtctgac gcgctgcacc atcccgtcgt tgttcgtgta ggtgccgtcc acctcggcga
     3361 acgaggccgc cggcagaacc acgtccgcat gctcggtcgt cgccgtgtgg aaaagctcct
     3421 gaacgacgac gaaatcgaac ttcgcg
//
LOCUS       DASVES010000309         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_590245, whole genome shotgun sequence.
ACCESSION   DASVES010000309 DASVES010000000
VERSION     DASVES010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590245"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF240_12530"
     CDS             complement(<1..303)
                     /locus_tag="VF240_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176079.1"
                     /translation="MSEKIDHVVTYESDEEVEDVLRRFESGALRPAEFKHRQHLTVAL
                     LYLLRLPEREVHARMRDTILRFLAHHRLDASVYHETITAFWLRRVRFYVRGADTERP"
     gene            complement(452..721)
                     /gene="rpsT"
                     /locus_tag="VF240_12535"
     CDS             complement(452..721)
                     /gene="rpsT"
                     /locus_tag="VF240_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7176080.1"
                     /translation="MPNHKSADKRMRQTEKRRVNNRTSRGRLRTGVKGLRAALEGGDA
                     AVVGEMLPKTISLIDKAVQKGVLHRNAAARHKSRLTLRANEAAKK"
     gene            1016..3055
                     /locus_tag="VF240_12540"
     CDS             1016..3055
                     /locus_tag="VF240_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7176081.1"
                     /translation="MKKIELPPRGIETLFGVHDQNIKYLESLLDVRIDARGQDLSIDG
                     DPKDVATAERIIEDFAALFGEGKTFTDKELREAFSQIAEDRAYSLHDYFTKARFNPAG
                     KKQVAPKSANQRRYIEAIQRDDIVFSIGPAGTGKSYLAVAMAVQALMLKQVSRIILAR
                     PAVEAGERLGFLPGDLQEKVDPYLRPLYDALFDLMDAERVTKMLEKRIIEVAPLAFMR
                     GRAQPLHSRVLTPDGWRAMGELCPGDAVIGSDGRPTQVLGVYPQGPKPVYRLTMTDGA
                     TTLACAEHLWAVLTPEDKRDGKPPRVLETRQMLDGLRRCHQHRYELPLLSAPVDWPRR
                     EVPLDPYSLGLLLGDGCITDKTTPGFSTGDPELVSSLRSGFGGMNLTFRRKSAVDYVL
                     ANPAAGHGGRIVRNPLTQALRGLELAGTRSSTKFIPEVYLYNSPEVRHAVLQGLLDTD
                     GGPVVQAGRTCRIQYTTTSRRLRDDVLFLVRSLGGVAYWRRRKAEGRKPGFANGREIG
                     YCNDAYVMDIRLPRGLEPFRLGRKTAVYHEHGGGRPMRFIESIDPAGTEQTQCISVAS
                     PDALYITDDFILTHNTLADAFIILDEAQNTTSEQMKMALTRIGFGSKAVVTGDVTQID
                     LPTGKRSGLIEAERILKGVEGISFIYFNEKDVVRHRLVQLIIKAYEQHTTRSGQV"
     gene            3161..3598
                     /gene="ybeY"
                     /locus_tag="VF240_12545"
     CDS             3161..3598
                     /gene="ybeY"
                     /locus_tag="VF240_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX7176082.1"
                     /translation="MADGVVEIVNRQRKLPVDGERWRAFTAKALKVAPAAGAGVTVAF
                     ISDRAMRELNRRWRGKRGTTDVLSFPAEQDAFEKSEGLSLGDVVISVEQAARQAGEHG
                     LSFDGEVAQLILHGLLHLCGYDHETDRGQMNALELRLRRRLGI"
BASE COUNT          651 a         1184 c         1148 g          616 t
ORIGIN      
        1 gggccgctcg gtgtcggcgc cgcggacgta gaagcgcacg cgccggagcc agaaggccgt
       61 gatcgtctcg tggtagacgc ttgcgtcgag ccggtgatgc gcgaggaagc gcaagatagt
      121 gtcccgcatg cgcgcgtgta cctcccgttc ggggagacgc agaaggtaga gcagcgccac
      181 cgtcaggtgc tgccggtgct tgaactccgc gggcctgagc gcccccgact cgaaccggcg
      241 cagaacgtct tcgacctctt cgtcgctctc gtaagtgaca acatggtcga tcttctcgga
      301 catactcgcc tccgtctctg aaagtgcgcg cgggcggcgg cacgcagtcg cgcccgaccg
      361 cacgcacgca aaaaggcgag gcggaccgaa aactgttttc ggtccgcctc gccggagata
      421 aaagttgtca gccgccgccc ggctgctgct gctacttctt ggccgcctcg ttggcgcgca
      481 gcgtgagccg cgacttgtgg cgcgcggcgg cgttgcggtg gagcacgccc ttctgtacgg
      541 ccttgtcaat cagggagatg gtcttgggca gcatctcgcc gaccacggcc gcgtcgccgc
      601 cttcgagggc cgcgcgcagt cccttgacgc cggtacgcag ccgtccacgg ctggtgcggt
      661 tattaacgcg gcgcttctcc gtctgccgca tgcgcttgtc tgctgacttg tgattgggca
      721 taacttcgga aaactccgtc ttctttaatg actccgatga atttagtgct gatttgcgcg
      781 cgcccctacg gatagaaggg aatttaccct ccggcgcaaa gggcaaagta tagggagggg
      841 gggcgtgagt gtcaaggcat gcgcccggag gtaaacccgt aagcacgtgg acgttgacac
      901 ccgcgccgcg cgtgttgtag cgtcatccac gaaccatgtc acagacgccc gttcagaacc
      961 ctcatccaaa cgcccccgcg gcgtccaacc acacgcaagg agtacacacc atctcttgaa
     1021 gaagatcgaa cttccgccga gggggatcga gacgctcttc ggcgtccacg accagaacat
     1081 caagtacctc gaatcgctcc tcgatgtacg catcgacgcc cgcggacagg acctgtccat
     1141 cgacggcgac cccaaggatg tggcgacggc cgagcgcatc atcgaggact tcgccgcgct
     1201 cttcggcgag ggcaagacct tcaccgacaa agagctgcgc gaagctttct cccagatcgc
     1261 cgaagaccgc gcctactcgc tccacgacta cttcaccaag gcccgcttca accccgccgg
     1321 caagaagcag gtcgccccga agtccgccaa ccagcgccgc tacatcgagg ccatccagcg
     1381 cgacgacatc gtcttcagca tcggcccggc cggaacgggg aagtcgtacc tcgcggtcgc
     1441 gatggccgtg caggcgctga tgctcaagca ggtgagccgc atcatcctcg cgcgcccggc
     1501 ggtcgaggcg ggcgagcgcc tcggcttcct gcccggcgac ttgcaggaga aggttgaccc
     1561 atacctccgc cccctctacg acgcgctctt tgatctgatg gacgccgagc gcgtcaccaa
     1621 gatgctggag aagcgcatca tcgaagtcgc gccgctggcc ttcatgcgtg ggcgcgcgca
     1681 accactgcac agtcgcgtgc tgaccccgga tggctggcgc gcgatgggcg aactgtgtcc
     1741 cggcgacgcg gtcatcgggt ctgacggcag gccgacgcaa gtgttgggcg tgtatccgca
     1801 ggggccgaag ccggtctacc gcctgaccat gaccgacgga gcgaccacgc tcgcgtgcgc
     1861 cgaacatctc tgggccgtgc tcacgcccga ggacaagcgc gacggcaagc ccccgcgggt
     1921 gctggaaact cggcagatgc tcgacggcct gaggcgttgt caccagcacc gttacgagtt
     1981 gccactgctt tccgctcccg tagactggcc gcgccgcgaa gtcccgctcg acccgtactc
     2041 gctggggctt ctgctcggcg acggctgcat caccgacaag accacccccg gcttttcgac
     2101 cggcgacccg gagttggtgt cgtcgctgag gtctgggttc ggcggcatga atctgacctt
     2161 ccggcgtaaa tccgccgtgg actacgtgct cgccaatccc gcggccggtc acggcggccg
     2221 tatcgttcgt aatccactta cacaggcgtt gcgcgggctg gagttggcgg ggacacgctc
     2281 ctccaccaag ttcatcccgg aagtctatct ctacaacagc cccgaggttc gccacgccgt
     2341 cttgcagggt ttgctggaca ctgacggcgg cccggtcgtc caggcgggac gcacttgcag
     2401 gattcagtac accacgactt cgcgtcggct cagggatgat gtcctgttcc tcgtgcgctc
     2461 gctcggcggc gtggcctatt ggcgcagacg caaagccgag ggccgcaagc cgggcttcgc
     2521 caacgggagg gaaatcgggt actgcaacga cgcttacgtg atggacatcc gcctgccgcg
     2581 tgggctggaa cctttccgcc tcggccggaa gactgccgtc taccacgaac acggcggcgg
     2641 gcgtccgatg cgcttcatcg agagcatcga cccggccggc acggagcaga cgcagtgcat
     2701 cagcgtcgcg tcgcccgacg cgctctacat caccgacgac ttcatcctga cccacaacac
     2761 ccttgcggat gcgttcatca ttctcgatga ggcgcagaac acgacgagcg agcagatgaa
     2821 gatggcgctg acgcgcatcg gcttcggctc gaaggccgtc gtcaccggcg acgtgacgca
     2881 gatcgatctg ccgacgggca agcgcagcgg cctcatcgag gccgagcgca tcctcaaggg
     2941 cgtcgagggc atctccttca tctacttcaa cgagaaggat gtcgtccgcc accgcctcgt
     3001 gcagttgatc atcaaagcct acgagcagca cacgacccgc agcgggcagg tatgaggaaa
     3061 gccatcagcc attagccgtt agctttcagc ttcgagaccg acgctcagat caacaggacg
     3121 tgggggcggc ccgcttaatt tggctgaatg ctgagagctg atggctgacg gcgtagtcga
     3181 gattgttaac cgccagcgca agctgcccgt tgatggcgag cgctggcggg cgttcacggc
     3241 gaaggcgctg aaagtcgcgc ccgccgccgg cgccggcgtg accgtggcct ttatctccga
     3301 ccgtgcgatg cgcgagctta atcggcgctg gcggggaaag cgcgggacga cggatgtgct
     3361 gtcgttcccg gcggagcagg acgcgtttga gaagtcggaa ggcttgagtc tgggcgacgt
     3421 ggtgatctcg gtcgagcagg ccgcgcggca ggccggcgag cacggcctga gtttcgacgg
     3481 cgaggtggcg caactcatcc tccacggctt gctccacctt tgcggctacg accacgagac
     3541 cgaccggggc cagatgaacg cgctcgaact ccgacttcgc cgccgtcttg gaatataat
//
LOCUS       DASVES010000310         8802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1416794, whole genome shotgun sequence.
ACCESSION   DASVES010000310 DASVES010000000
VERSION     DASVES010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416794"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VF240_12550"
     CDS             complement(<1..198)
                     /locus_tag="VF240_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176083.1"
                     /translation="MAEHPEGIVLTTVDSIFNSLRLKVGAPVMNLKPVRDTVNWARKS
                     ALWPMTFGLACCAIEMIATGAG"
     gene            complement(288..713)
                     /gene="ndhC"
                     /locus_tag="VF240_12555"
     CDS             complement(288..713)
                     /gene="ndhC"
                     /locus_tag="VF240_12555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX7176084.1"
                     /translation="MTSAILLQATSATASTAGGPVTAYWPVMIFFVFAIIFPVLPILL
                     GRVVRPSLWQKAKMRPYECGIDPVTEAHDRFTVRYYIVAMLFLIFDVETIFLLPWAII
                     FMGEDFSFTKFALIEMFVFLGILVVGYYYAWRKGALEWA"
     gene            complement(840..1355)
                     /locus_tag="VF240_12560"
     CDS             complement(840..1355)
                     /locus_tag="VF240_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX7176085.1"
                     /translation="MNSPVQIRAVVITVSDACARGVREDVSGEALARLLREAGAEVVA
                     HEVLADDLEPLAEALRTHADRAEVNLVVTTGGTGLTPRDNTPEATREAIEREAPGLAE
                     AMRAGTLAKTPTAMLSRGVCGVRSGALIINLPGSPAGVRECFEVIRPVLAHAVALLAG
                     RDDGSHRESRA"
     gene            complement(1408..4662)
                     /gene="carB"
                     /locus_tag="VF240_12565"
     CDS             complement(1408..4662)
                     /gene="carB"
                     /locus_tag="VF240_12565"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7176086.1"
                     /translation="MPRRSDIHKILIIGSGPIVIGQACEFDYSGTQACKALRQEGYEV
                     VLVNSNPATIMTDPETADRTYIEPLSLEAVTAIIKRERPDALLPTVGGQTALNLAMAL
                     DEAGVLEEFGVEMIGAKREAIELAEDRELFKRAMDEEGLPMPLGGFAHSWAEAEKIVE
                     TTGYPAIIRPSFTMGGAGGGTAYNPEEFEEIVRNGLATSPVRQVLIEESILGWKEFEL
                     EVMRDLADNVVIICSIENFDPMGVHTGDSITVAPAQTLTDVEYQRLRDIAIKVIRKVG
                     VETGGSNIQFAVNPATGEVRVIEMNPRVSRSSALASKATGFPIAKIAAQLAVGYTLDE
                     IANDITKKTPASFEPTIDYVVAKIPKWNFEKFRGAEDSLGTQMKSVGEVMAIGRTFKE
                     ALFKGLRSLEAVKPLRLQDVPDDELQRKLARPNSQRFSYITYALKHDWTVAEVYRLSG
                     IDPWFLEQLRDVMDVEDDTEGRKLEEISAEQLRVFKEVGLSDRRLAYLTNRSEDEVRA
                     HRKRLGVIPVYKRVDTCGAEFESFTPYMYSTYEEEDESAPTDRRKIMILGSGPNRIGQ
                     GIEFDYCCCHASFALREAGFETIMVNCNPETVSTDYDTSDRLYFEPLTFEDVMNIVDV
                     ERPEGIIVQFGGQTPLNLALRLSEAGAPIIGTSPDSIDLAEDRKRFGALLSELGIPQP
                     EAGTATSPEEAKECAARIGYPVLVRPSYVLGGRAMAIVYDERSLDDYMRTAVDASPEK
                     PVLIDKFLERAAEFDVDALADETAVVVAAIQEHIEEAGIHSGDSSCVLPPVRIAPEHV
                     ETMRHYTRVLARALDVRGLLNIQYAIKDDRVYVLEVNPRASRTVPFVSKATGVPLARI
                     AALVMAGQRLDSFNLPDDLTVGRFFIKSPVFPFVKFPGVDPILSPEMHSTGEVMGVGS
                     TFGEAYAKAMTGAGLELPREGTAFISVNDADKGQAVVLARRLSRLGFQLTATYGTAAR
                     LREVGLEVESVFKVNEGRPNIADKIKQGEIALVINTPLGMASHYDEQAIRRAALQYGV
                     ACVTTMTGAQAIIEAITARESRETTVITLQELHEESSKFKVQSSKAGSEAAFMP"
     gene            4844..5434
                     /locus_tag="VF240_12570"
     CDS             4844..5434
                     /locus_tag="VF240_12570"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7176087.1"
                     /translation="MAANPERRYTLEEYFELERKSEERFEFWGGEIFCMSGVSPEHDQ
                     IESNLNYHLRAKLAGRSCRVFLANMRIKVPAAPPYRYADLSALCGEPKFEEIGGVGAL
                     TNPALVVEVLSPSTEAYDRGDKFTHYKSIPSFGEYLLVAQHRPHITQFVRQDDGSWVY
                     KEFNDLGESLKLSSLDCELSLGEVYQNVSFNSEASD"
     gene            complement(5640..6476)
                     /locus_tag="VF240_12575"
     CDS             complement(5640..6476)
                     /locus_tag="VF240_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176088.1"
                     /translation="MSSDKAKKNPPLFAVPDREESVSDTAPSPTRAPGGQEAPQVCPL
                     CFGTGHEVVPGRGARRCQCRTGGQSQKLVDAARIPRRYREPAPPRKEPCSLQNYYPLP
                     NNGSQLKAFNYAFRLVRDYPAVERGLLFTGPVGVGKTHLAVAILHGLLEKGVPCLFYE
                     FGSLLKEIQDSYNPISKNSELRVLAPVYQAEVLVLDELGASKPTDWVRDTMMQIINTR
                     YNDKKLTVFTTNYLDARRDPKDETLEDRVGVRLRSRLYEMCKSVHIDGDDYRKTLDSG
                     IR"
     gene            complement(6767..7672)
                     /locus_tag="VF240_12580"
     CDS             complement(6767..7672)
                     /locus_tag="VF240_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176089.1"
                     /translation="MRTSPTKTHASLVALTAAIFISAAAAAQGQRDAAARKTAGAEQT
                     VVFAVQKYEAEAQIDPVAVISGGRYAAPPLGGDTPLETARFKRFVRDFYRPGRKFRVL
                     FGGGEAGSVEVRKYLEPGCVGMEASVTLQTSARLGGQVAALATNSAALGRGKGTRRAP
                     TDAERTAALELARRAFGRNKVAAARVAKMETNNLTAADLNGDGRAELIGSFIITGEFG
                     VEDALLLIAEPGIDDFAVTLDWFHHGAEADSHYRRLVDVLDLDGDGAAEVIVQGIYYE
                     SHDYFIYKKQRGAWRVVYQGGGGGC"
     gene            complement(7701..7850)
                     /locus_tag="VF240_12585"
     CDS             complement(7701..7850)
                     /locus_tag="VF240_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176090.1"
                     /translation="MKCLYCARPLRMGGLWCRACRRYVLRWPHIAMLAVAGFAVLYAL
                     LELVG"
     gene            8071..8529
                     /locus_tag="VF240_12590"
     CDS             8071..8529
                     /locus_tag="VF240_12590"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HEX7176091.1"
                     /translation="MSIFHGLLGRLGIRDAAAPPRGQLRVFLLDDDSQRHDWFAKRFK
                     GDTLDVATEPARAIELLSENQYDAVFLDHDLLPEHYYAEEFDDERTGYAVANWLAANP
                     ERQSASYIVVHTRNADGAMRMVEMLRRVGRHAEYVPFPLLTQKIKSAWRR"
     gene            8545..>8802
                     /locus_tag="VF240_12595"
     CDS             8545..>8802
                     /locus_tag="VF240_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176092.1"
                     /translation="MRRVHAPARPFLRLALLLLCPALGHAEAFAQENKPAGERHAEQV
                     KTLLASPEIVKALAHIDSGREQILKEWIAITEVNAPSGKERP"
BASE COUNT         1565 a         2683 c         2913 g         1641 t
ORIGIN      
        1 ccccgccccc gtggcgatca tctcgatggc gcagcaggcc agtccgaacg tcatcggcca
       61 cagcgccgac ttgcgcgccc agttcaccgt gtcgcgcacc ggcttcaggt tcatcaccgg
      121 cgccccgacc ttcagccgca gcgagttgaa gatcgaatcg accgtcgtga gtacgatgcc
      181 ttcgggatgc tcagccatat tcaggtgatg agtgataagt gatgagtgat gagaaggata
      241 agacggggtt tgtcttatca cttatcactt atcacatatc acgcctctca tgcccactct
      301 aaggcgccct tgcgccaggc gtagtagtag ccgacgacga ggatgccgag gaagacgaac
      361 atctcgatga gggcgaattt ggtgaaagaa aagtcctcgc ccatgaagat gattgcccac
      421 gggaggagga agattgtctc cacatcgaag atgagaaaga gcatggcgac gatgtagtag
      481 cgcacggtga agcggtcgtg cgcctcggtc acggggtcga tgccacactc gtaggggcgc
      541 atcttcgcct tctgccagag gctcggacgg acgacgcggc cgaggaggat cggcagcacg
      601 gggaagatga tggcgaagac gaaaaagatc atcaccggcc agtaggccgt gacggggccg
      661 cccgcggtcg aggcggtggc ggacgtagct tgcaggagaa tcgcgctcgt catggtcagg
      721 tgtaaacccc ggcgcgccgt cggatgtcgg aacgctcgtg attctaggca gccgcgcgtt
      781 atgtgtcaac aaacattact gccgggccgg cctccgcgtt gatgggagcg acgcttcttt
      841 caggcgcggc tttctcggtg ggagccgtcg tcgcgcccgg cgagcagggc gacggcgtgg
      901 gcgaggacgg ggcggatgac ctcgaagcac tcgcgcacgc ccgcgggcga ccccggcagg
      961 ttgatgatga gggcgccgga gcgcacaccg cacacgccgc gcgagagcat ggccgtcggc
     1021 gtcttggcga gcgttccggc gcgcatcgcc tcggcaagtc ccggcgcttc tcgctcgatg
     1081 gcctcgcgcg tcgcctcggg cgtgttgtcg cgcggagtga ggccggtgcc gccggtcgtc
     1141 acgacgagat taacttccgc gcggtcggcg tgtgtccgca aagcttcggc cagcggttcg
     1201 aggtcgtccg ccaaaacctc gtgcgccacc acttcagcgc ccgcctcgcg cagcagacgg
     1261 gccagcgcct cgcccgacac gtcctcacgc acgccgcgcg cgcaagcgtc gctcaccgtg
     1321 atgacgaccg ccctaatctg gacaggagag ttcaaagttc aaagtcgaaa gttcaaagcc
     1381 ggaggttcaa agttcaaagt ttgtagttca aggcatgaag gcggcttcgg agccggcctt
     1441 tgaactttga actttgaact ttgaactctc ctcgtgcagc tcttgcaggg tgatgacggt
     1501 ggtctcgcgg ctttcgcggg cggtgatggc ttcgatgatg gcctgtgcgc cggtcatcgt
     1561 cgtcacgcag gcgacgccgt actggagcgc ggcgcggcgg atggcctgct cgtcgtaatg
     1621 ggaggccatg ccgagggggg tgttgatgac gagggcgatc tcgccctgct tgatcttgtc
     1681 ggcgatgttc gggcggccct cgttgacctt gaagaccgac tcgacttcga ggcccacctc
     1741 gcgcagacgc gcggccgtgc cgtatgtcgc cgtgagttgg aagccgagtc gggagaggcg
     1801 tcgggcgagc acgaccgcct gccccttgtc ggcgtcgttg acgctgatga aggccgtgcc
     1861 ctcgcgtggc agctccagcc ccgcgcccgt catcgccttc gcgtaagcct cgccgaaggt
     1921 cgagccgacg cccatcacct cgcccgtcga gtgcatctcg ggcgagagta tcggatcgac
     1981 gccggggaac ttgacgaacg ggaacaccgg cgacttgatg aagaagcggc cgaccgtcaa
     2041 atcatccggc aggttgaaac tatcaagccg ctggcccgcc atcacgagcg cggcgatgcg
     2101 cgcgagcggc acgcccgtcg ccttcgagac gaagggcacg gtgcgcgagg cgcgggggtt
     2161 tacttcgagg acgtagacgc ggtcgtcctt gatggcgtac tggatgttga gcaatccgcg
     2221 cacgtcgagc gcgcgcgcga gcacacgcgt gtagtggcgc atcgtctcga cgtgttcggg
     2281 cgcgatgcga acgggcggga gcacgcaaga ggagtcgccc gagtgaatgc cggcctcctc
     2341 gatgtgctcc tggatggcgg cgacgacgac ggccgtttcg tcggcgagcg cgtccacgtc
     2401 gaactcggcg gcgcgctcca gaaacttgtc gatgagcacg ggcttctcgg gcgaagcgtc
     2461 aacggccgtc cgcatgtagt cgtcgagcga gcgctcgtca tacacaatcg ccatcgcgcg
     2521 tccgccgaga acgtaactgg ggcggacgag gacggggtag ccgatgcgcg cggcgcactc
     2581 cttcgcctct tcgggcgacg tggccgtgcc cgcttcgggt tgcgggatgc cgagttcgct
     2641 gagcagcgcg ccgaagcgtt tgcggtcttc ggcgaggtcg atggagtcgg gcgaggtgcc
     2701 gatgatgggc gcgcccgctt ccgaaagtcg cagcgcgagg ttgagcggag tctgcccgcc
     2761 gaactggacg atgattccct cggggcgctc cacgtccacg atgttcatca cgtcctcgaa
     2821 ggtgagcggc tcgaagtaga gacggtcgga ggtgtcgtag tcggtcgaga cggtctcggg
     2881 gttgcagttg accatgatgg tctcgaaacc ggcctcgcgc agggcgaacg acgcatgaca
     2941 gcaacagtaa tcgaactcga tgccctgccc gatgcggttc ggccccgagc cgaggatcat
     3001 gatcttgcgg cggtcggtgg gcgcggactc gtcctcctcc tcgtaggtcg aatacatgta
     3061 gggcgtgaac gattcgaact cggcgccgca cgtgtccacg cgtttgtaga cggggatgac
     3121 gcctaagcgt ttgcggtggg cgcgcacctc gtcctcactc cgattcgtga ggtaggcgag
     3181 gcggcgatca gagaggccga cctccttgaa gacgcgcagt tgttcggcgg aaatctcttc
     3241 gagcttgcgg ccctcggtgt cgtcctccac gtccatcacg tcgcggagct gttcgagaaa
     3301 ccaggggtcg atgcctgaga ggcgatagac ttcggcgaca gtccagtcgt gcttgagcgc
     3361 gtaggtgatg taagagaagc gttgcgagtt ggggcgggcg agcttgcgct gcaactcgtc
     3421 gtcgggcacg tcctgtaggc gcaggggctt gacggcttcg agcgaacgca gacctttgaa
     3481 gagcgcttcc ttgaaggtgc ggccgatggc catcacctcg ccgaccgact tcatctgcgt
     3541 ccccagagag tcttccgccc cgcggaactt ctcgaagttc cacttcggaa ttttggcgac
     3601 gacgtagtca atcgtcggct caaaactcgc cggtgtcttc ttggtgatgt cgttggcgat
     3661 ttcgtcgagc gtgtagccga cggcgagctg ggcggcaatc ttggcgatgg ggaagccggt
     3721 cgccttcgag gccagcgccg aggagcggga gacgcgcggg ttcatctcga tgacgcgcac
     3781 ctcgcccgtg gcggggttga cggcgaactg gatgttggag ccgcccgtct cgacgccgac
     3841 cttgcggatg actttgatgg cgatgtcgcg caagcgttga tattcaacgt cggtgagcgt
     3901 ttgcgccggg gcgaccgtga tggagtcgcc ggtgtggacg cccatcgggt cgaagttctc
     3961 aatcgagcag atgatgacga cgttgtcggc gaggtcgcgc atcacctcca gctcaaactc
     4021 cttccagccg aggatggact cttcgatcaa gacctgtcgg acgggcgacg tggcgagtcc
     4081 gtttctgacg atctcctcaa actcctcggg gttgtaggcc gtgccgccgc ccgcgccgcc
     4141 catcgtgaag gagggccgga tgatggcggg gtagccggtg gtctcgacga ttttttccgc
     4201 ctcggcccac gagtgcgcga agccgcccaa cggcatcggc agaccctcct cgtccatcgc
     4261 ccgcttgaac agctcgcggt cttcggccaa ctcgatggcc tcgcgcttcg cgccgatcat
     4321 ctcgactccg aactcttcga gcacgccggc ttcgtcaagc gccatcgcga ggttgagcgc
     4381 cgtctgtccg ccgacggtgg gcagcaatgc gtcaggccgc tcgcgtttaa taatggcggt
     4441 gacggcttcg agcgacagcg gctcgatgta ggtgcggtcg gccgtctccg ggtcggtcat
     4501 gatcgtcgcc gggttggagt tgactaagac gacctcgtag ccctcctgcc gcagggcctt
     4561 gcacgcctgc gtccccgagt agtcgaactc gcacgcctgc ccgatgacga tggggccgga
     4621 gccgatgatg aggattttgt ggatgtctga gcgtctgggc atctatcaag cgtcggaacc
     4681 gattgtggtc attttgggtt attttgtggt catggcatta tacataacga cccgatggag
     4741 aacgaaccgc cctccactcg gcgaagtgcg gacgacgacc gtcggcaacg atgcttgatg
     4801 atgattccaa acctcacgcg cggacggaga aggagtttga gatatggccg ccaacccgga
     4861 gcgacgctac acgttggaag aatatttcga gctggagcgc aaatccgaag aaaggtttga
     4921 attctggggc ggcgaaatct tttgcatgag cggcgtcagc ccggagcatg accagataga
     4981 atcgaatctc aactaccacc tcagagcaaa actcgcaggg cgtagttgtc gcgtctttct
     5041 ggctaatatg cgaatcaagg ttccggccgc cccgccttac cgctatgcgg acttgtcggc
     5101 cctgtgcggc gaaccgaaat ttgaagagat cggcggcgtc ggcgctttga ccaacccggc
     5161 tttagtcgtc gaagttttgt cgccctcgac ggaagcttac gaccggggcg acaagttcac
     5221 gcattacaaa tccatcccga gcttcgggga atacctgctc gtcgcccagc accgcccaca
     5281 cattacccag ttcgtcaggc aggacgacgg ctcctgggtc tacaaagagt ttaacgacct
     5341 cggagagtct ctgaaactgt cctcgctcga ttgcgagctg agtctgggcg aggtttatca
     5401 gaacgtctcc ttcaactcgg aagcgagcga ttgaacttct tttccaaacc attaagcatc
     5461 cgcccgtttc atcaatgtag gggcagggct tgtccgcggc cctcgttcac gattcataca
     5521 acgtggaatc aatcgtacct acagggacag ccatgcccct acagatgaca cgggcgcgag
     5581 ttgctctgag ttggcgagag tgttttgccg gagggattga cgggtcgaag cgtctcactt
     5641 cagcggatgc cggagtcgag ggtcttgcgg tagtcgtcgc cgtcgatgtg gacggacttg
     5701 cacatctcgt agaggcgcga gcggaggcgg acgccgacgc ggtcttccag cgtctcgtcc
     5761 ttggggtcgc ggcgggcgtc gaggtagttg gtggtgaaga cggtgagctt cttgtcgttg
     5821 tagcgcgtgt tgatgatctg catcatcgtg tcgcgcaccc agtcggtcgg cttcgacgcg
     5881 ccgagttcat cgagcacgag cacttcggcc tgatagaccg gcgccagaac cctcagctcg
     5941 gagttcttcg agatggggtt gtaggaatcc tgaatctcct tgaggagcga gccgaactcg
     6001 tagaacaggc acggcacgcc cttctccagc aagccgtgga ggatggcgac ggcgaggtgc
     6061 gtcttgccga cgccgaccgg ccccgtaaag agcaggccgc gctctacggc cgggtagtcg
     6121 cgcacgagtc ggaaggcgta gttgaacgcc ttgagctgcg agccgttgtt ggggagtggg
     6181 tagtagttct ggagcgagca cggctccttg cgcggcggcg ccggctcgcg gtagcggcgc
     6241 gggatgcgcg cggcgtccac gagcttctgc gactgcccgc ccgtgcggca ctggcagcgg
     6301 cgcgcgcccc gtccggggac gacctcgtgg ccggtgccga agcagagcgg gcagacttgc
     6361 ggcgcttcct gaccacccgg cgcgcgcgtc ggcgacggcg cagtgtcgga gacagactcc
     6421 tccctgtcgg gcacggcgaa gaggggagga ttttttttcg ctttgtctga cgacatcttc
     6481 ggcgcttcgg tggcgactgc ttttcgttta ttgcacgagg ccgtgtcggg gcgagctgtg
     6541 cgcgtgcttc gacgcacccg cactcaaaat ccggtcacgc gagcgcgggc gaagcgtgcg
     6601 ggattatagc atccgaaaag tagattgcaa ggccgtcttt ccgggcgacg gatttccaca
     6661 ggtggaaaac aaaaggcggt gatcagtgat gagtgataag tgataagaga aagcaaccgt
     6721 catgtcattt cttatcactt atcacttatc actgatcacc gccttttcag catccgcctc
     6781 cgccgccctg atagacgacg cgccacgcgc cgcgctgttt cttgtagatg aagtagtcgt
     6841 ggctctcgta gtagatgccc tgcacgatga cttcggcggc gccgtcgccg tcgaggtcga
     6901 gcacgtccac gaggcggcga tagtgggagt cggcctccgc tccgtggtgg aaccagtcga
     6961 gggtcacggc gaagtcgtcg atgcccggct cggcgatgag caggagcgcg tcctcgacgc
     7021 cgaactcgcc cgtgataatg aaagagccga tgagttcggc gcggccgtcg ccgttgaggt
     7081 ctgcggccgt gaggttgttc gtctccatct tcgcgacgcg cgcggccgcg accttgttcc
     7141 ggccgaaggc gcggcgggcg agttcgagcg cggcggtacg ctccgcatcg gtgggtgcgc
     7201 ggcgtgtgcc cttgcctcgg ccgagcgcgg cggagttggt ggcgagcgcc gccacctgtc
     7261 cgccgaggcg cgcggaggtc tgcaatgtga cactcgcctc catgccgacg cagcccggtt
     7321 cgaggtactt ccgaacctcg accgaccccg cctcgccccc gccaaatagc acacggaact
     7381 tccggcccgg ccggtagaag tccctgacga agcgtttgaa gcgcgccgtt tcaaggggag
     7441 tgtcgccgcc gagcggcggc gccgcgtagc gtccgccgga gatgacggcg accgggtcga
     7501 tctgcgcctc cgcttcgtac ttctgcacgg cgaagacgac cgtctgctcc gcgccggccg
     7561 tcttgcgcgc ggcggcgtcg cgctgtccct gggccgcggc ggcggcggat ataaaaatgg
     7621 ccgccgtgag ggcgaccaga ctggcgtgcg ttttcgtcgg cgacgttctc atctgtgaat
     7681 cacctgtggc ctcggccctg ctagccaacc aattccagta gcgcatagag gactgcgaag
     7741 ccggcgacgg cgagcatcgc gatgtgcggc cagcgcagca cgtagcggcg gcacgcgcgg
     7801 caccacagac cgcccatgcg tagagggcgc gcgcagtata aacacttcat ctgaaggtca
     7861 ccatatttcc ttcatcaagt gcgcggtcgc cggcgcgcgc tatcgcgcgg cggggcgtgt
     7921 gagacgcctc atgtttgtga ggatagcaat gaggtattaa cggcagagaa tgtggtatag
     7981 tttctgccgc tgacaatcat acttcattcg ggcgcggcga cgaagcttta tcttcgtgca
     8041 ccggcgttcg atattttaca gaattaccgc atgagtatct ttcacgggtt gctggggagg
     8101 ctcggcatcc gagacgccgc cgcgccgccg cgcgggcaac tccgcgtctt cctcctcgac
     8161 gacgattcac aacgccacga ctggttcgcc aagagattta agggcgacac acttgatgtc
     8221 gccacggaac cggcgcgcgc catcgaactg ctctccgaga accagtacga cgccgtcttt
     8281 ctcgatcacg acctcctgcc cgaacactac tacgccgaag agttcgatga cgagcgcacg
     8341 ggctacgccg tcgccaactg gttggccgcg aaccccgagc ggcagtcggc ctcgtacatc
     8401 gtcgtccaca cgcgcaacgc cgacggcgcg atgcggatgg tcgagatgct ccgccgcgtc
     8461 ggccgccacg ccgagtacgt ccccttcccc ctactcacgc agaagatcaa gagcgcctgg
     8521 aggcgttaga ccccacagca caccatgaga cgagttcacg cgcccgcacg ccccttcctg
     8581 cgcctcgcgc tcctgctcct ttgccccgcg ctcggccacg ccgaagcgtt cgctcaggag
     8641 aacaagccgg ccggcgaacg ccacgccgag caggtcaaga ccctgctcgc cagccccgaa
     8701 atcgtcaagg cgctcgcgca catcgattcg ggccgcgaac aaatcctcaa ggagtggatc
     8761 gccatcacgg aggtcaacgc cccctcgggc aaggagcggc cg
//
LOCUS       DASVES010000311         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1135814, whole genome shotgun sequence.
ACCESSION   DASVES010000311 DASVES010000000
VERSION     DASVES010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135814"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..>2718)
                     /locus_tag="VF240_12600"
     CDS             complement(102..>2718)
                     /locus_tag="VF240_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HEX7176093.1"
                     /translation="TTAEWQTHVGSFKVNPACQAPAQGTLQVNVTDCDSNLPLAGARV
                     FIGGNLYGTSGAGGSFSTQLPPGVHNVSVTLPNYSVGVGAATVTDGGATVIDLCAAPS
                     PFITPAGSALTAESCPPPTDAIDPGETVTVDLTLKNTGAANTSNLVATLLPTGGVTSP
                     SGPESYGVVVAGGPSVTKSFTFTASGALACGDQITATLQLQDGPDDLGAVTYTFDTGA
                     LGAPTSASYSSGNLATALPDNTTVDIPINVADTGAVDDVNVRVRLNHTFDGDVEIRLV
                     HPDGTVVLLSDNRGGSGDNYGAGANDCSGTHTVFDDGAAVAISAGTAPFAATFRPEQP
                     LSALNGKATNGTWKLRITDTANLDTGTVGCVQLEINRRQRLCCPFAGGTPAVVAAPPA
                     DLTAESCAPANDAIDPEETVTVELPLKNNGTGPTTNLVATLLPGGGISPVTTSQNYGA
                     LSPVGAGSTASRPFTFVAQGACGSTVTATLQLQDGANNLGAVTFTFVLGAVVLNTTTH
                     ANAAPITILDTPRVGGIAPSSPYPSAINVSGVTGTVSKVSARLNNFSHTFPGDVDVLL
                     VGPGGQKLMLMSDMGSGTDAVNATLTFDDTAPAIGAAVVTGAFRPTNSGTGDIFPAPA
                     PAGPYPDPQLLSVFNGVNPNGTWSLYVVDDAGTDAGSITGGWELIITTEQRVCCDSPC
                     TITPPANVVVNNDAGSCGAIVNYPAATVGGSCGVLSYSHPSGSFFPVGTTTVTITATR
                     ADSTTSTDTFTVTVNDAEGPSVSDPVASPSSLWPPNHKMRDVTVSYGATDNCTTPGAI
                     SCTLSVTSNEPVNSTDDGDTAPDWQVVNNHLVRLRAERSGSGSGRIYTITVTCTDSAG
                     NQTVKTTTVTVPYSL"
BASE COUNT          366 a          808 c         1097 g          447 t
ORIGIN      
        1 gacgggggcg cgccgcgcga cggaagtttg gaaggtgtgt gttcggcggc ggaggaggac
       61 gaacggcagt ttgatcattc gtcctcctcc gcccggcagt cctacaggct ataaggcacg
      121 gtcaccgtgg tggtcttcac cgtctgattc ccggcggagt ccgtgcaggt gaccgtgatc
      181 gtgtagatgc gtcccgagcc gctgcccgag cgttcggcgc gcaggcggac gaggtggttg
      241 ttgacgacct gccagtcggg cgccgtgtcg ccgtcgtccg tgctgttgac gggctcgttg
      301 ctcgtgacgc tgagcgtgca actgattgcc cccggcgtgg tgcagttgtc cgtcgcgccg
      361 tagctgaccg tcacgtcccg catcttgtgg ttcggcggcc acaacgacga gggcgaggcg
      421 acggggtcgg agacggacgg cccctcggcg tcgttgaccg tgacggtgaa ggtgtccgtc
      481 gaggtggtgc tgtcggcccg cgtcgccgtg atggtgacgg tggttgttcc gacggggaag
      541 aacgagcccg acgggtggct gtaggagagc acgccgcaac tgccgccgac ggtggccgcc
      601 gggtagttga caatcgcgcc gcaggagccg gcgtcgttgt tgacgacgac gttcgcgggc
      661 ggcgtgatcg tgcacggcga gtcgcagcag acgcgctgct cggtggtgat gattagctcc
      721 cagccgccgg tgatgctgcc ggcgtcggtg ccggcgtcgt caaccacgta gaggctccac
      781 gtgccgttcg ggttgacgcc gttgaagacc gagagcagtt gcgggtcggg gtaggggccg
      841 gccggcgcgg gcgcggggaa gatgtcgccc gtgccggagt tggtcggcct gaaggcgccg
      901 gtgacgacgg ccgcgccgat ggccggggcc gtgtcatcga aagtcagcgt ggcgttcacg
      961 gcgtcggtgc cgctgcccat gtcggacatc agcatcagct tctgcccgcc cggcccgacg
     1021 agcagcacgt caacgtcgcc ggggaaggtg tggctgaagt tattgagccg cgccgacacc
     1081 ttgctgaccg tgccggtgac gcccgagacg ttgatggccg acgggtaggg cgacgacggc
     1141 gcgatgccgc cgacgcgcgg cgtgtcgagg atggtgatcg gcgcggcgtt cgcgtgggtc
     1201 gtcgtgttca agacgaccgc gccgaggacg aacgtgaagg tgacggcgcc gaggttgttg
     1261 gcgccgtcct gaagttggag cgtcgccgtg acggtgctgc cgcacgcgcc ctgcgcgacg
     1321 aaggtgaacg gccgcgacgc cgtggagccg gcgccgacgg gactgagcgc gccgtagttc
     1381 tgcgacgtgg tgacgggcga aatgccgccg cccggcagaa gcgtggcgac gaggttggtg
     1441 gtcgggccgg tgccgttgtt cttgagcggc agctcgaccg tgacggtctc ctcggggtcg
     1501 atggcgtcgt tggccggagc gcaactctcg gccgtcaagt cggccggggg ggccgcgacg
     1561 accgcgggcg tgccgccagc gaacgggcag cagagccgct gccgccggtt gatctcaagc
     1621 tgcacgcagc ccaccgtccc cgtgtcgagg ttcgccgtgt cggtgatgcg cagcttccac
     1681 gtgccgttcg tcgccttgcc gttgagcgcg gagagcggct gctcgggcct gaaggtggcg
     1741 gcgaaggggg ccgtgcccgc gctgatggct acggccgccc cgtcgtcgaa gacggtgtgc
     1801 gtccccgaac agtcgttcgc gccggcgccg tagttgtcgc cgctgccgcc gcggttgtcc
     1861 gagaggagga cgaccgtgcc gtccgggtgg acgagacgta tctctacgtc gccgtcaaac
     1921 gtgtggttga gacgcacgcg cacgttaacg tcatcgacgg cgcccgtgtc ggcgacgttg
     1981 atggggatgt ccaccgtggt gttgtcgggg agggcggtgg cgaggttgcc gctgctgtag
     2041 gaggcggagg tcggcgcgcc gagggcgccg gtgtcgaagg tgtaggtgac ggcgccgagg
     2101 tcgtcggggc cgtcctggag ttgcagggtg gccgtgatct ggtcgccgca ggcgagcgcg
     2161 cccgaggcgg tgaaggtaaa cgacttcgtc accgaggggc cgccggccac gacgaccccg
     2221 taagactcgg ggccgctcgg cgaggtgacg ccgccggtcg ggagcagtgt ggcgacgagg
     2281 ttcgaggtgt tggccgcgcc ggtgttcttc agcgtgaggt caaccgtgac ggtctcgccg
     2341 gggtcgatgg cgtcggttgg gggcggacag ctctcggccg tcagcgcgga gccggcgggc
     2401 gtgatgaagg gcgacggcgc ggcgcagagg tcgatgaccg tggcgccgcc gtcggtgacg
     2461 gtggcggcgc cgacgccgac cgaatagttg ggaagcgtga ccgagacgtt gtggacgccg
     2521 ggcggcagtt gggtggagaa ggagccgccc gccccgctcg tgccgtagag gttgccgccg
     2581 ataaagacgc gcgcgcccgc gagcgggagg ttcgagtcgc agtcggtgac gttgacctgg
     2641 agcgtgccct gcgcgggggc ctgacaggcc gggttgacct tgaaactgcc gacgtgagtc
     2701 tgccactcgg cggtcgtc
//
LOCUS       DASVES010000312         8008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_397723, whole genome shotgun sequence.
ACCESSION   DASVES010000312 DASVES010000000
VERSION     DASVES010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397723"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..3050
                     /locus_tag="VF240_12605"
     CDS             270..3050
                     /locus_tag="VF240_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176094.1"
                     /translation="MAALLALCVVLQGAAAQTVTPSQAGGARQRFEYLWMEAENMRGL
                     ATDARNEPRIYASYVLHKRESAPGWGINGPGVSAEWSQGGESEWNSVAASPDEVKATI
                     YRDVEIPRDGRYRVWVRYADWGGRDEEFAVRVRQGGSDASTRAEVFNRRYGTADRLDP
                     RDEVVMYWGWAFAWEGSDAEPLRKGAARVSIDIERASSARRHVDCILITDDLSYVPEG
                     RRKPPFAALRVLKEWGEKRTPLASLAPVERATSDAAPAVATVPPLWRRRPLAGRDFVV
                     PWNISENFWELYGSAAPEQRPLYPFNAEPHDLFIEKYKGVKDVPIFSSKLVVPVVYIN
                     NLPKHLKEGSPFLKYLRETRAPFGFNINYGAASMSAPDAQAAWALLNGELREQFIGWV
                     SGESIGHVYEDAAPNLTLTPDMTRARMLEGWRSAYDAALQRKWGALFQTQTGPMWDKL
                     IPAQATSSTTYAHALTRWGVRLLGLETAAVQPNFALRVAFTRGAARQYDANFLYYHAP
                     NFGDTATTFTHAQNFAGPAHFFHSRYGASMGPSLSWYRKCYFFYYMAGASAVYLEQGF
                     DQFFKPAPGEHPFQLNPLGRITDEFVRFAERHPERGTPYTPVAFLLDPAHGFEMTDYP
                     HWPFGVSQIERGDHALRELFGAAYYPAPETEGEPATADRQAFVNSIFGDLFDVLVASD
                     DRRDAADAYRTIIVGGRVDFSQAWVEKLIDHVQKGGTLLVNAEQARWFPEQLLGVRGK
                     GATAEAADARCLSKGETPADLSGQTFRYERVEPRGAEALLAAPTGDALVTVNRVGRGR
                     VVYCAIPDLLGLDERLAPAAAHTLVHLLADAAPVRVRGDVQHLVNRNERGWVVTLINN
                     RGVYKQQQGLAQVNREEAVDVTLDLRGRTVAGASEWTTDTQLGVEREGGGRVRLSVPP
                     GGVRIVELITGK"
     gene            3265..3540
                     /locus_tag="VF240_12610"
     CDS             3265..3540
                     /locus_tag="VF240_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX7176095.1"
                     /translation="MKVARRFIVSGEVQGVGFRFFAQRVASRHQVTGYVRNLADGTVE
                     AHAEGTPEGVEGFRHDISVGPGHARVSQVEEISTEPTGLYPTFRIER"
     gene            3642..4163
                     /locus_tag="VF240_12615"
     CDS             3642..4163
                     /locus_tag="VF240_12615"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX7176096.1"
                     /translation="MDDLKQIIREVPDFPKPGINFYDITTLLKHPEGFRRTIDALVAQ
                     FEGDKVDTVVGIEARGFIFAPAMAYRLGTGFVPARKPGKLPSKCASVSYDLEYGQDAL
                     QIHSDAVGAGHRVLIADDLLATGGTAAAVVKLVEQLGGEVVGLTFVVELEFLSGRQRL
                     EGYDVRSLIKYQS"
     gene            4212..4287
                     /locus_tag="VF240_12620"
     tRNA            4212..4287
                     /locus_tag="VF240_12620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4245..4247,aa:Arg,seq:cct)
     gene            4361..4834
                     /locus_tag="VF240_12625"
     CDS             4361..4834
                     /locus_tag="VF240_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176097.1"
                     /translation="MSRPATTLVIVILAFIAPVFASAAMDGRDDVWQSHQRARPAQGS
                     EEVFITDIEMARDDGAGRGGQTVRSFRAKDNPLHCLVTLSRAQQGTRVGFVWTALDAG
                     GKRDEAIASAEVVTGPREIVADSQLRHPREWPAGRYSVQATINGRAARTVEFTIN"
     gene            4976..5407
                     /gene="umuD"
                     /locus_tag="VF240_12630"
     CDS             4976..5407
                     /gene="umuD"
                     /locus_tag="VF240_12630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEX7176098.1"
                     /translation="MDTKVEAVFWPERATRWARPLFLAAVSAGFPSPADDYIEGRLDL
                     NRHLIRHPVATFYVRVAGDSMIDAGIHPGSILVVDRAVEADDGDIVIARLGDELCVKR
                     LRIHEGRLLLVPENPCYETIEVGEGMDFEVWGKVMHSIRSY"
     gene            5491..6753
                     /locus_tag="VF240_12635"
     CDS             5491..6753
                     /locus_tag="VF240_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEX7176099.1"
                     /translation="MFAVVDCDNFYVECERVFAPALRRRPVVVLSNNDGCVISRSAEA
                     KALGIGMGAPLFQVRDVVSEQGVRVFSSNYALYGDMSGRVMQTLASLSPEVEVYSIDE
                     AFVRFDAAGRKGKPLDHAHAVRERIRRWTGVPVSIGLGPTKTLAKVAVRLAKRSGAES
                     VYSLDGGAATEEALANLAVGEVWGIGRKRARVLHDAGIETALGLSRADGRWLRRRLSI
                     VTLRTAYELRGVSCLPLECCPRPKRSITTSRSFGRPVETLPEIQEALAYFVTKAAEKL
                     RRARLAARVLHVFLMTNRFEPETFYAGAATVPLSVPTDLTPELIGHARGAAARAFREG
                     CQYRKAGVMLLDLVDARPAQAGLFDDRDRERARRVVRAVDEINARLGEGAVRFGAAGG
                     AGRPWRTRCESRSPRYTTCWRELLTLNT"
     gene            complement(6772..7179)
                     /locus_tag="VF240_12640"
     CDS             complement(6772..7179)
                     /locus_tag="VF240_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7176100.1"
                     /translation="MSEGGDARQSFYSAEVERLARENEDFRRVLFTAQYAQLVLMAIP
                     PGEEIGEEVHDRVDQVLVFVAGEGEAVIEGERSSVRAGGLCIVPSGTRHNFIAGAGSP
                     LKLFTVYSPPEHPDGTVHRTKAEADEYEREHHH"
     gene            complement(7266..7664)
                     /locus_tag="VF240_12645"
     CDS             complement(7266..7664)
                     /locus_tag="VF240_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7176101.1"
                     /translation="MMLNLLFRKLFPRRDADDAASRADFGAESDAPEGDEVDPFNPFP
                     EPVRLEITDVLDLHAFSPRDVRRVVEEYLLEARARGFRQVRIIHGKGVGVQREAVRSV
                     LARTPFIEHFADAPPEAGGWGATVARLSSE"
BASE COUNT         1381 a         2705 c         2600 g         1322 t
ORIGIN      
        1 atgtacacct gccgtcggcg tacacttgcc gaatgcctcg acgcgctcgg gtgagcggcc
       61 gcgatacaga cacgctcagc ggacgcggag ggacaacaag cccctccgcg tctcttcgtt
      121 tcaggcaaca ccacaccaat gccctccgcg ccttggcgtc ctctgcgtta aattttcgac
      181 ccaaaggaaa tttaacgcag aggacgccaa ggcgcggagg acgctgagat gagcgtagtt
      241 cgcacaatat gagtcgcaga acaaatagcg tggcagcact gttagccctg tgcgtagtgc
      301 tacagggcgc ggcggcgcag actgtgacgc cgagtcaggc gggcggcgcg cgtcagcgtt
      361 tcgaatacct ctggatggag gccgagaaca tgcgcggcct cgcgacggac gcgcgcaacg
      421 agccgcgcat ctacgcctcc tacgtcctgc acaagcgcga gagcgcgccg ggatggggca
      481 tcaacgggcc gggcgtctcc gccgagtggt cgcagggcgg cgagagcgag tggaactccg
      541 tggcggcgag tcccgacgag gtgaaggcca cgatttacag ggacgtggaa atcccgcgcg
      601 acgggcgtta ccgcgtctgg gtgcgctacg cggactgggg cggccgcgac gaggagttcg
      661 ccgtgcgcgt ccggcagggc ggaagcgacg cctccacgcg tgccgaagtc tttaaccggc
      721 gttacggcac ggccgaccgt ctcgacccgc gcgacgaggt cgtcatgtac tggggctggg
      781 cgttcgcctg ggaaggctcc gatgcggagc cgcttcggaa gggcgcggcg cgcgtctcca
      841 tcgacatcga gcgcgcatcg agcgcgcgcc gtcacgtcga ttgcatcctc atcacggacg
      901 acctctcata cgtccccgag gggcggcgca agcccccgtt tgccgccctt cgcgtcctga
      961 aggagtgggg cgagaagaga actcccttgg cgtcgctcgc gccggtcgaa cgggcgacaa
     1021 gtgatgccgc gccagcagta gcaacagtgc cgccgctctg gcgtcgccgc cctctcgccg
     1081 gccgcgactt cgttgtgccc tggaacatct cggaaaattt ctgggagcta tacggcagcg
     1141 ccgcgccgga gcagcgtccg ctttatccgt tcaacgccga gccgcacgat ctgttcatcg
     1201 agaagtacaa gggcgtgaag gacgtgccca tcttctcatc aaagctcgtc gtcccggtcg
     1261 tctatatcaa caacctgcca aagcatctca aggagggcag ccccttcctc aaatacctgc
     1321 gcgagacgcg cgcgcccttc ggcttcaaca tcaactacgg cgcggcgagc atgagcgcgc
     1381 ccgacgcgca ggcggcgtgg gcgctcctga acggcgagct gcgcgagcag ttcatcggct
     1441 gggtttcggg cgagagcatc ggccacgtct acgaggacgc agcgcccaac ctgacgctga
     1501 cgccggacat gacacgcgcc cggatgctcg aaggctggcg ctccgcctac gacgccgcgc
     1561 tccagagaaa gtggggcgca ctcttccaga ctcagacggg gccgatgtgg gacaagctca
     1621 tcccggcgca ggccacttcg agcaccacct acgcgcacgc gctcacgcgc tggggagtcc
     1681 gcctccttgg gcttgagaca gcggccgtcc agcccaattt cgccctgcgc gtcgccttca
     1741 cgcgcggcgc cgcccgccag tacgacgcaa acttcctcta ctaccacgca cccaacttcg
     1801 gcgacaccgc cacgaccttc acccacgcgc agaacttcgc cggccccgca cacttctttc
     1861 acagccgcta cggagcctcg atggggccgt cgctatcgtg gtatcgaaag tgttacttct
     1921 tctactacat ggccggcgct tcggccgtct atctcgaaca gggcttcgat caattcttca
     1981 agcccgcccc cggcgaacac ccgttccagc tcaacccact gggacgcatc accgacgagt
     2041 tcgtgcgctt cgccgagcga caccccgagc gcggcacgcc ctacacgccc gtcgccttcc
     2101 tgctcgaccc cgcgcacggc ttcgagatga ccgactaccc gcattggccc ttcggcgtct
     2161 cgcaaatcga acgcggcgac catgccctgc gcgagctgtt cggcgcggcc tactaccccg
     2221 cgcccgagac cgaaggcgag ccggcgacgg ccgaccgtca ggcgttcgtc aactcaatct
     2281 tcggcgacct cttcgacgtg ctcgtcgcct ctgatgaccg tcgcgacgcg gccgacgcct
     2341 accgcaccat catcgtcggc gggcgcgtcg atttctcgca ggcgtgggtc gaaaaactca
     2401 tcgaccacgt gcagaagggc ggcacgcttc tggtcaacgc ggagcaggcc aggtggttcc
     2461 cggaacaact cctcggcgtg cgtgggaagg gcgcgaccgc cgaggccgcc gacgcgcgtt
     2521 gcctctcgaa gggtgagacg ccggccgacc tttcgggcca gacgtttcgc tacgagcgcg
     2581 tcgagccacg cggcgcagag gcgctgttgg ccgcgccgac gggcgacgct ctcgtcaccg
     2641 tgaatcgcgt cgggcgcggc cgcgtcgtct actgcgccat ccccgacctc ctcggcctcg
     2701 acgagcgtct cgcgccggcc gccgcgcaca ccctcgtaca cctcctcgcc gatgccgcgc
     2761 ccgtccgcgt tcgcggcgac gtgcagcatc tcgtcaaccg caacgaacgc ggctgggtcg
     2821 tgacgctcat caacaatcgc ggcgtctaca aacagcagca ggggctggcg caggtgaacc
     2881 gtgaagaggc cgtcgatgtc acgctcgacc tgcgcggtcg caccgtcgcg ggcgcgagcg
     2941 agtggacgac cgacacacaa ctcggtgtcg agcgcgaagg cggcgggcgc gtcaggctca
     3001 gcgtgccgcc gggcggagtt cgtatcgttg agttgattac cggcaagtag gaaggggagc
     3061 gatgcttaat gcaacgacag taccgcgagc ggtagcgagc gggtgtcgat gcgcgaggtg
     3121 ggcctcccat tgtaagcaca cccgctcgcc agccgtactg tcctcattcg gaaagccccg
     3181 gtttgcattt acgacggttg ttcttttagc atagcgcgtc ttaccgaaag gccgcgccgc
     3241 atcgagcaag tggttctcag tagaatgaaa gtcgcacgca ggtttatcgt cagcggggag
     3301 gtgcagggcg tcggcttccg cttcttcgcg caacgcgtcg cctcccgaca tcaggtgacg
     3361 ggctacgtgc gtaacctcgc ggacggcacg gtcgaggccc acgccgaggg gacgcccgaa
     3421 ggcgtcgaag gcttccgcca cgacatctcc gtcggccccg gccacgcacg cgtctcgcaa
     3481 gtcgaagaga tcagcacgga gccgacaggg ctttacccga cattcaggat tgaaaggtga
     3541 cgaacaagaa ttcaggagtc agaagtcagg agtcagaatg agaatagcgg ccctatcttt
     3601 tattctgact cctgactcct gactcctgac tcccaaagct tatggacgac ctaaaacaaa
     3661 tcatccgcga agtgcctgac tttccgaagc ccggcatcaa cttctacgac atcaccacgc
     3721 ttttgaagca ccccgagggg ttccgtcgca ccatcgacgc gctggtcgcg cagttcgagg
     3781 gcgacaaggt ggacaccgtg gtcggcatcg aagcgcgcgg cttcatcttc gcgccggcga
     3841 tggcctaccg actcggcacg ggcttcgtcc ccgcgcgcaa gcccggcaag ctcccatcga
     3901 agtgcgcctc ggtctcctac gatctggagt acggacagga cgccctccag attcacagcg
     3961 acgccgtcgg cgccggccac cgtgtcctca tcgccgacga cctgctggcc acgggcggca
     4021 cggccgcggc cgtcgtcaag ctcgtcgaac aactcggcgg cgaggtcgtc ggtctcacct
     4081 tcgtcgtgga gctggaattc ctgtccggtc gccagcggct cgaaggctac gacgtgcgct
     4141 cgctcatcaa gtatcagtcg tgagtccggc tggacgcttc taactcgctt ctgtaaactt
     4201 tcccgcggtg tgctcccgta gctcagtgga tagagcacct gcctcctaag cagggggccg
     4261 cacgttcgag tcgtgccggg agcaccatca aacctctccc cgtgtgtgat tcacttcgag
     4321 tccatcggct cattcccgag ccgcctgcgc ggtgtttaga atgtcacgtc ctgcgaccac
     4381 gttagtcata gtcatcctgg ccttcatcgc gcccgtcttc gcgtccgcgg cgatggacgg
     4441 ccgggacgac gtgtggcagt cccaccagcg cgcacgcccc gcgcagggca gcgaagaagt
     4501 cttcatcacg gacatcgaga tggcgcgcga cgacggcgcg ggacgcggcg ggcagacggt
     4561 caggagtttc cgcgcgaagg acaatccgct ccactgtctc gtcaccctca gccgcgcgca
     4621 gcagggcacg cgcgtcgggt tcgtgtggac ggccctcgac gcgggcggca agcgcgacga
     4681 ggcgattgcc agcgccgaag tcgtgacggg gccgcgcgag atcgtcgccg actctcagtt
     4741 gcgccacccg cgcgagtggc ccgccgggcg ttacagtgtg caggcgacca tcaacggacg
     4801 cgcagcgcgc acggtcgagt tcacgatcaa ctgagcgggc ctccgccaga ttgacctctc
     4861 cgccctcaag ctcacacgct cagacacccc tcccgaataa aagtcttgta aattccggcc
     4921 cgttgcgcgt acaattccgg cgctggcaca atctaccaca gatgaaacaa ggcgtatgga
     4981 cacgaaagtc gaagccgtgt tctggccgga gcgcgcgacg cgctgggcgc gcccgctctt
     5041 tttggcggcg gtctccgcag gctttcccag tcccgccgac gactacatcg agggccgcct
     5101 cgacctcaac cgacatctta tccgtcaccc ggtcgccacc ttctacgtcc gcgtcgccgg
     5161 cgactcgatg atcgacgccg gcatccaccc cggctcaatc ctcgtcgttg accgcgccgt
     5221 cgaagccgac gacggcgaca tcgtcatcgc gcgtctgggc gacgagttgt gcgtcaagcg
     5281 cctgcgcatc cacgaaggcc gcctcctgct cgtacccgag aacccatgct acgagaccat
     5341 cgaggtcggc gaggggatgg acttcgaggt gtggggcaag gtcatgcact cgattcggtc
     5401 ttactaaagg gcaaggggga agggcatatc gcgggcggtg ccgtctcgtt ccttcttcat
     5461 tcatatcttt tcccgctccg cttgctgacc atgttcgccg tcgtcgattg cgataatttt
     5521 tacgtcgagt gcgagcgcgt cttcgcgccg gcgctgcggc gacggcccgt ggtcgtgctg
     5581 tcgaacaacg acgggtgcgt catctctcgc tcggccgagg cgaaggcgct cggcatcggg
     5641 atgggcgcgc cgctctttca ggtgcgggat gtcgtgagtg aacagggcgt ccgggtcttc
     5701 tcgtcgaact acgcgctcta tggcgacatg tcggggcgcg tgatgcagac gctcgcgagc
     5761 ctttcgcccg aggtcgaagt ctactcgatt gatgaagcct tcgtgaggtt cgacgcggcg
     5821 ggccggaagg gtaagccgct cgaccacgcg cacgccgtgc gtgagcgcat caggcgctgg
     5881 acgggcgtgc ccgtgagcat cggcctcggg ccgacgaaga cgctcgccaa ggtggccgtg
     5941 aggctggcca agcggagcgg ggcggagagc gtctacagtc tcgacggggg cgcggcgacg
     6001 gaggaagcgc tggcgaacct ggcggtcgga gaggtgtggg gcatcggccg caaacgcgcg
     6061 cgcgtcctcc acgacgccgg catcgaaacc gcgctcggac tcagccgcgc cgacgggcgc
     6121 tggctgcgtc ggcgtctgag catcgtcacg ctgcgcaccg cctacgagtt gcgcggcgtc
     6181 tcgtgcctgc cgctcgaatg ttgcccgcgc ccgaagcgtt cgattaccac ctcgcgctcc
     6241 ttcgggcgcc ccgtcgagac gctgccggag atacaggagg cgctggctta ctttgtgacg
     6301 aaggcggcgg agaagctgcg gcgcgctcgg ctggccgcgc gcgtcctcca cgtcttcctg
     6361 atgacgaacc gcttcgagcc ggagaccttc tacgccggcg cggcgaccgt ccccttgagc
     6421 gtcccgaccg acctcacgcc cgagttgatc ggccacgcgc gcggggcggc cgcgcgcgcc
     6481 ttccgcgagg gctgccagta tcgaaaggcc ggggtgatgc tgctcgacct ggtggacgcg
     6541 aggccggcgc aggcggggtt gttcgacgac cgcgaccgcg agcgcgcccg gcgcgtcgtc
     6601 cgcgccgtcg atgagatcaa cgcgcgtctg ggcgaaggcg ccgtccgctt cggcgccgcc
     6661 ggcggagcgg ggcggccgtg gcgcacgcgc tgcgaaagtc gttcgccgcg ttacaccacc
     6721 tgctggagag agcttctgac gctcaacacc taaacggaag ctgccacggc ttcagtggtg
     6781 atgctcccgc tcgtactcgt cggcttccgc cttcgtccgg tgaaccgtgc cgtccgggtg
     6841 ttcaggcggg gagtagacgg tgaagagctt gagcggagag ccggcgccgg cgatgaagtt
     6901 gtggcgcgtc cccgagggga cgatgcagag gccgccggcg cgcacgctcg accgctcgcc
     6961 ctcgatcacg gcctcgccct cgcccgcgac gaacaccagc acctgatcta cgcggtcgtg
     7021 tacctcctcg ccgatctcct ctcccggcgg gatggccatc agcacgagct gcgcgtactg
     7081 cgcggtgaat agcacgcgcc tgaagtcttc gttctcccgc gcgagccttt cgacctccgc
     7141 ggagtaaaaa ctctgcctcg cgtcgccgcc ctcactcata aacacactct cccgtctgtt
     7201 aaagatttcg gaaaaacttt gcgttgatta tcgcccgcga tggcggagga gggaagcggc
     7261 ggcgttcact ccgatgagag gcgcgcgacc gttgcgcccc agccgccggc ctcggggggc
     7321 gcgtccgcga agtgttcgat gaagggcgtg cgcgccagca ccgagcgcac cgcctcgcgc
     7381 tgtacgccga cgcccttgcc gtgaatgatt cgcacctgac ggaagccgcg cgcgcgggcc
     7441 tccagcagat actcttcgac gacgcgccgc acgtcgcgcg gcgagaaggc gtgcaggtcg
     7501 agcacgtccg tgatctccag acggactggc tcggggaaag gattgaaggg gtcaacctcg
     7561 tcgccttcgg gcgcgtcgga ctctgcgcca aagtcggcgc gcgacgccgc gtcgtcggcg
     7621 tcacggcgcg gaaaaagttt gcgaaacagg aggttgagca tcattgaaat aactccacgt
     7681 cggttgccgg cggcggccgc cgccgaacgc cttgactctg gcccgcgccc accttaaact
     7741 cgcgattctt caaggaaaat catacaacaa cgggcaggcc agtgcgcact cgggcgcggg
     7801 cggcggtgcc cgcgcgaggg gctggcagtc tccgtcagca atccgcaatt taacatccga
     7861 ccgaaggaaa ggaacctgaa gagatgggca gacgaaacaa gatcacggtg gtgggggccg
     7921 gcaacgtcgg cgcgacggcc gcgcattggc tggcgtcgaa agagttgggc gatgtggtgc
     7981 tcgtggacgt ggtcgagggc gtgccgca
//
LOCUS       DASVES010000313        15341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_12837, whole genome shotgun sequence.
ACCESSION   DASVES010000313 DASVES010000000
VERSION     DASVES010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15341
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12837"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VF240_12650"
     CDS             <1..1310
                     /locus_tag="VF240_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7176102.1"
                     /translation="ELFGESRANGTLLQTNRTYFRQLSVGGDLPTRRLGSFSFRAYGG
                     TQVFDQNFTAVAADRNSETLTRMQRVPSQFIGLTVQWSRAVGASQTLVVGLDTREVRG
                     ASDEIVFVQNRPSSLVGAGGRERTVGFFVEDIVRLTPRLFLTGGVRVDRWREFAAQSA
                     TRPVGQTAATVNVFADRTETAISPRLSVLYKPGENISLSLAAYRAFRQPSLNELYRSF
                     RVGDVLTLSNENLRAERMAGGEAGASVVTFNGRLNARGTFFWAEITRPIANVTLMVTP
                     NLITRQRQNLGRTRSRGLELDAEARINDRWSLTGGYLFVDARVRSFPANAALEGLLIP
                     QVARHQLTFQLRYTNPARLTVGLQGRAAGSQFDDDQNLFRLDRFFTLDALASRPVAGG
                     VEIFIAGENLFNRRYALGRTPVTTVSPPLLIRVGLRLRFGAAR"
     gene            1449..2216
                     /gene="ygiD"
                     /locus_tag="VF240_12655"
     CDS             1449..2216
                     /gene="ygiD"
                     /locus_tag="VF240_12655"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX7176103.1"
                     /translation="MPSIFFGHGNPMNALARNTWTDGWAAIGGEVPRPRAVLCVSAHW
                     YLPATLVTASPAPRTIHDFGGFPRKLYEVKYTAPGDPELARRVQELLAPVSVNLDQRW
                     GLDHGTWSVLCHVFPEADVPVIQLSIDETQPAEFHYETAKRLGSLRDEGVLIVGSGNL
                     VHNLHAYAWGRQQVEPLDWAVRFEERARELLLAGDHGPLIAYETLGRDAALSAPTPDH
                     YLPLLYVMAQQREGDEISFPVEGFDGGSVSMLSVKIG"
     gene            complement(2231..2650)
                     /locus_tag="VF240_12660"
     CDS             complement(2231..2650)
                     /locus_tag="VF240_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176104.1"
                     /translation="MNTKVLMVASSLALGLAGIAASFAPSEMLRAVGSPAVEPLPVLI
                     QLLGGTYLGFAMTNWTAKDNIIGGIYSRPVSLGNSVLFLTGALALAKQQFSSGFRLPL
                     TAALIVYTIFAIWFMWLVLGHGTACQVAAPERKGAGY"
     gene            complement(2838..3737)
                     /locus_tag="VF240_12665"
     CDS             complement(2838..3737)
                     /locus_tag="VF240_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7176105.1"
                     /translation="MKLLIAADSAVSTEVLVGAVGVRPWPDGTTAHVLSVVVDADVPP
                     EVWREEGYGKATVQREMERREEQITTIAVERLKEVGIPAEVLVTRGDPRQLIPFFARK
                     WSSELIFVRAHVRKNLAHWMLGSVARAVVAAAPCTVQIVRDPAEDRAHTLESGRRVLL
                     ATDGSETSAAAAQALAGRPWPEGSEFRVLSVEEPWAIKPSRVRHDEQAQEAVRSAEQI
                     LASAGLKVTMAVSVSDNAKEVILEEARKWAADLIVVGSHGRRGFKRFLLGSVSEAVAM
                     NAHCSVVVVRGPARPSRKGRASG"
     gene            complement(3788..3943)
                     /locus_tag="VF240_12670"
     CDS             complement(3788..3943)
                     /locus_tag="VF240_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7176106.1"
                     /translation="MGQAIPIGKLCEPGDNACAMLFPASDEAKYIVGQTLGVDGGRTL
                     PASVSAV"
     gene            complement(4550..5443)
                     /locus_tag="VF240_12675"
     CDS             complement(4550..5443)
                     /locus_tag="VF240_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7176107.1"
                     /translation="MRLLLAADSTVTTEMLMNAVASRPWPRGTKARVLSVVEDEAIPP
                     EVWREAGYRVDAVHQEMRRREGQITALTVEPLEELGITAEVAIRRGDPQWLIPQEARN
                     WPADLILIRAHNRTDFRSWMLGSVAKSVVRDAPCSVEVVRAFRDDRSVVANGHMKILL
                     ATDGSEHAAVAARAVAEQPWPEGTEVKVMSMVNPLAYSIEEIGLYDGGGTRRAHRAIG
                     DAAQILAGAGVKTSAEVIAGRPDRRIAREANEWGADLIVVGTEDRRGLRRLLRGSVSE
                     RVASRAPCSVKVVRVRDEDSR"
     gene            complement(5466..5591)
                     /locus_tag="VF240_12680"
     CDS             complement(5466..5591)
                     /locus_tag="VF240_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176108.1"
                     /translation="MSLPFTRDGETYRTCMGCGARRQFDTERWETVGQFYHPARP"
     gene            complement(5725..6057)
                     /locus_tag="VF240_12685"
     CDS             complement(5725..6057)
                     /locus_tag="VF240_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176109.1"
                     /translation="MPEFEQLREKIVVEEPVAMEAPRPPGDLAVEVSATVRNTTRRTL
                     SGLEMRGAVLDARGSALRERTVIVVPARQTALEPGEAMGVRILLEGVRPEAERAGVLM
                     EVSGVRFD"
     gene            complement(6754..7389)
                     /locus_tag="VF240_12690"
     CDS             complement(6754..7389)
                     /locus_tag="VF240_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7176110.1"
                     /translation="MSKQLLVVDDDPSLLLAVSETLRAEGYEVVTARRGAEAMVRVAE
                     ALPDLIISDIRMPGMDGYALARNLRSAPRTRLVPIVFLTAKDETADRIAGFRTGVDAY
                     LTKPFEPEELVAVVKSILDRVQRTHSDLARMFGEQETDDVELVRDEDLTDAEWRVAEA
                     VARGLSNKEIAAELHLSLRTVEGHISRILDKKSLSNRVELALHVREHKQHD"
     gene            complement(7364..9205)
                     /locus_tag="VF240_12695"
     CDS             complement(7364..9205)
                     /locus_tag="VF240_12695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176111.1"
                     /translation="MSIRGRLLALALGAAVPLVLLGLAMLWVVWGAKEQQLNESLEQQ
                     AELAAVVFDRWLDAQRQPLITIASYPAGHFTDAAALEADLRAATARRPHWIDVRALDA
                     GGRVVAAFPSDAESLPAGVAERLIAEAHGGEAAIETDWTRGEGRYVLAVAAPMEGGGA
                     CIARVDGKALGDLFHGIALPERALVITLLDSHRRVVYRSQTPESALGLDLSDAGVLSA
                     LGDRATAVVTVTSTIDGVERTYGLAHAGRTGYVTMVGVPSAALYAPARRQLTTYALVS
                     LAVVFGTLTAALLVARSIARPVRLLGFAAQRFGEGNFSARAPDAGADEISRLGVSFNA
                     MAERLEQREARMAELDSLKSDFVSSVSHELRTPLTTIKALTRLLMRDGLDDAKRREYL
                     DTISVECDRQIDLVLNLLDLSRLEGGVFRITHERVDVAELVSSVVKSEARAAEKRSHE
                     LRVEPVEDISNVCADPKALRRVLSNIIENAIKYTPDGGRIQLSARADGDEVLISVTDN
                     GRGIPPEDMPVLFDKFHRGRPATHSEAMRNATTDAEFLEDADVSGVGLGLYLGRNVME
                     QMGGKISVESEVGRGSTFTLHLPVWRDGGCRKQPEVEDRNVKTVTGR"
     gene            9198..10505
                     /locus_tag="VF240_12700"
     CDS             9198..10505
                     /locus_tag="VF240_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7176112.1"
                     /translation="MLNRRLQVLSSVLVYFLLSITNRPDFHDKRKHLRSVRDNTLALD
                     RRTNYDWQIKRAPIMNDTGVVIMDWGVNSRAVRGKAIMDHLRNGRQSSFGESSRAAEL
                     PGSRRADRWAVILAGGDGTRLRPLTRLIAGDERPKQFCPVIGDQTLLDQTRNRVALAV
                     RPDQTIFVVTQHHARFYKPLLGDVRRQQLVAQPKNVGTASAILYGLLRLSQVAPSSSV
                     AFFPSDHYFSDDDRFMSHVESAFEAAQVCEDLVILLGIQPDGPEVEYGWIEPASPGLM
                     KNPDALCRVHRFWEKPTTHMAHALMDMGCLWNSFVMVGRVSAFLEMIRRAAPELYGRF
                     DAVRPVLNTADEGRAIDGLYAQLRETNFSQQVLAARPEDLAVLPVSGLRWSDLGQPGR
                     VLSTMADIGLEPSRGAVLGFSHAAESRGQRVNLLTRDREALTR"
     gene            10502..10765
                     /locus_tag="VF240_12705"
     CDS             10502..10765
                     /locus_tag="VF240_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176113.1"
                     /translation="MIFRTGGTFYRGASAVEIARALERDAADYPRPGQTLRQFLLWSL
                     NHLLDRVPPRELDLSDRLDDEALAFSYLCLCDEYDAGELTVEP"
     gene            10998..12419
                     /locus_tag="VF240_12710"
     CDS             10998..12419
                     /locus_tag="VF240_12710"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7176114.1"
                     /translation="MNTTQPHPSFFDNVSLYFDHAASFTQHPEGLLDQIKVCNSVYSF
                     QFPVRTQRGIEVITGWRAQHSHHKLPVKGGIRYSEDANEDEVKALAALMTYKCAVMDV
                     PFGGGKGAVKINPKNYTVEELEQITRRYTSELIKKNFIGPGVDVPAPDYGTGQREMSW
                     IVDTYAAFHPGQIDALACVTGKPVSQGGVRGRLEATGRGVFFGLREACGDGEDMKRLG
                     LDRGLEGKRVVVQGLGNVGYHAAQFCQQGGAVITGLAEYEGAIYSAKGLDLEAVMKHR
                     KETGSLLNFPGASNLGSREEALELECDILIPAALENQLTEENAGRVKAKILAEAANGP
                     TSAGAEEILLRKGVLVIPDIYLNAGGVTASYFEWLKNLSHVRFGRMGKRFDQTTFENM
                     LRVVETTTGKKLSDEEIKRIARGADEIDLVNSGLEETMVVAYHQIHEIWKADSKIPGL
                     RTAAFISAINKIATSYAELGIFP"
     gene            12503..13036
                     /locus_tag="VF240_12715"
     CDS             12503..13036
                     /locus_tag="VF240_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7176115.1"
                     /translation="MMKSGEHKIATADAGNGQYPVEDVESSEPLELEVERLQEELAAE
                     RERGLRALAEFDNYRRRVRRESASAEEAGKRDIILALLDVMDDFDRALAHVSDAPDAV
                     ADGLRLIHQRLSRVLDSNGVTPFQSAGEQFDPTVHEAISVVESDGHESDTVYEEERRG
                     YLWNGELLRPARVIVVR"
     gene            complement(13121..13531)
                     /locus_tag="VF240_12720"
     CDS             complement(13121..13531)
                     /locus_tag="VF240_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7176116.1"
                     /translation="MVSPDEGRQAATDPQVEQELRRVNDEWAKALAQRDRAALDRIMA
                     DDFMFAYPFEGDDKEQFIADIVAGEVRVESLEPHDATVRVYGGAGVIFGSETANWQYR
                     DRDFSGTYKFFRMYARRQGRWQIVTLHLCPAAHR"
     gene            complement(13748..14392)
                     /locus_tag="VF240_12725"
     CDS             complement(13748..14392)
                     /locus_tag="VF240_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7176117.1"
                     /translation="MDEGLVRFGLTAFVTLLVVVDPPGVVPIFVALTEGADAAKRRAT
                     VKRAVIIALLVALFFLVAGRSLLSYLGVTIHAFAISGGVLLFATALPMLFGHRGGLQA
                     PEREERPTAGEDIAIFPLAIPLLSGPGAIATILLLTAQANGDTRRLVALGLAVVLVYG
                     VSLIMLRLGGSLMARIGEGKVHIVTRVMGIILAALAVQFILNGITAYYELLTGR"
     gene            complement(14476..14715)
                     /locus_tag="VF240_12730"
     CDS             complement(14476..14715)
                     /locus_tag="VF240_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176118.1"
                     /translation="MGKENTAMPDPIARKRLKKKVLDRWENEGGRISADPTGVDESTP
                     ASGHEGEGKQLSASQDNSAVDTPASPTKKRKPTRQ"
     gene            complement(14762..15172)
                     /locus_tag="VF240_12735"
     CDS             complement(14762..15172)
                     /locus_tag="VF240_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176119.1"
                     /translation="MLTPELLIEGSERLLVTSTTIVKPASSRVITHQGQVFSRYLLSY
                     LSAKLLTSKRNARDERGVVRRHHKLLKEAIMDLSAIIVVALGILIFFGGVAWLEMRSR
                     KSNRPARQDAQPQQPDDLTTSARRTVSRRAAMSE"
BASE COUNT         2952 a         4814 c         4631 g         2944 t
ORIGIN      
        1 gcgagctttt cggcgagtca cgcgcgaacg gaacgctgct gcaaaccaac cgcacctatt
       61 ttcgacagtt gagcgtcggc ggcgatctgc cgacaagacg actcggcagt ttcagcttta
      121 gagcttatgg cggcacgcag gtcttcgatc aaaatttcac agcggtcgcc gccgaccgga
      181 acagcgagac cctgacgcgg atgcagcgcg taccgtcgca attcatcggg ctgacggtcc
      241 agtggtcgcg cgccgtcggc gcgagtcaga cgctggttgt cggtcttgat acgcgcgaag
      301 ttcgtggcgc gagcgacgag atcgtcttcg tccagaaccg cccgtcatcg ctcgtcggtg
      361 ctggcgggcg cgaacggact gtgggtttct tcgtggaaga catcgtgcgt ttgacgccgc
      421 gcctcttcct cacgggcggc gtgcgcgtgg atcgctggcg cgagtttgct gcgcaatccg
      481 caacgcgccc ggtcgggcaa acggcggcga ccgtgaacgt ctttgccgac cggactgaga
      541 ccgccatcag cccgcgcctg tccgtcctct acaaaccggg cgagaacatt tccctctcgc
      601 tcgccgctta ccgggcattc cgtcagcctt cgcttaacga gttgtataga agtttccgcg
      661 tcggcgacgt gctgacgctc agtaatgaaa acctgcgcgc cgaacgcatg gcgggcggag
      721 aggccggcgc aagcgtcgtc accttcaacg ggaggcttaa cgcgcgcgga acttttttct
      781 gggcggagat cacgcgcccc atagcgaacg tcacgttgat ggtgacgccg aacctcatca
      841 cgcggcagag gcagaatctg ggtcgcacgc gctcgcgggg gttggagctg gacgcggagg
      901 cccgcatcaa cgaccgctgg tctctgacgg gcggttacct gttcgttgat gcgagggtgc
      961 gcagcttccc ggccaacgcg gccctcgaag gactcctcat tccgcaggtg gcgcgccatc
     1021 agctcacctt tcaattgcgc tacacgaatc cggcgcgcct caccgtaggt ttacaagggc
     1081 gcgcggcggg cagccagttt gacgacgacc aaaacctgtt tcgacttgat cgcttcttca
     1141 ccctcgacgc tcttgcttcg cggcccgtcg cgggcggcgt ggaaatattc atcgcggggg
     1201 agaatctatt caatcggcgt tacgcgctcg ggcggacgcc ggtgacaacc gtcagcccgc
     1261 cgctgttaat tcgtgtgggt ttgaggctgc gctttggcgc ggcacgctga cgccggtcga
     1321 gctgatgaat tcaacacaga ccggcaaggt tgtcgtctac tgacacggga ggttgaaatg
     1381 agcgccgaga aaaacatgga gttgatgcag accctgttaa gcgaggggga atgaacatgt
     1441 ctacacggat gccttccatt ttcttcgggc acggcaaccc gatgaacgct ctggcacgta
     1501 acacgtggac tgacggctgg gccgccatcg ggggagaggt tccgcgcccc cgtgccgtgc
     1561 tttgcgtctc cgcccactgg tatctcccgg ccactctggt gactgccagc cctgcgccgc
     1621 gcaccatcca cgacttcggc ggcttcccgc gaaagctgta cgaggtcaag tacacggcgc
     1681 caggcgaccc ggagctggcg cgccgcgttc aagaactgct cgcgccggtc tcagtcaacc
     1741 tggaccagcg gtggggactt gaccacggca cgtggtcggt gctctgtcac gtcttccctg
     1801 aagcggacgt gcctgtcatc caactcagca tcgacgagac gcagccggcg gagttccact
     1861 acgaaacagc gaagcgactc ggcagcttgc gtgacgaagg ggtgctcatc gtggggagcg
     1921 gcaacctcgt acataacctg cacgcctacg cctggggccg tcagcaggtc gagccgctcg
     1981 actgggccgt gcgcttcgag gaacgagcgc gtgagctgct gctcgccggc gaccacggcc
     2041 cgctcatcgc ctacgagact ttagggcgtg acgccgcctt gtcagctccg acgcccgatc
     2101 actatttgcc tcttctctac gtgatggcgc aacagagaga aggcgacgag atcagcttcc
     2161 cggtcgaggg attcgacgga ggatcagtct ctatgctctc agtcaaaatc ggctaagtta
     2221 ggtttcggta ctaatagcca gcgcctttcc tctcaggggc ggccacctgg cacgccgtcc
     2281 catgcccgag cacgagccac atgaaccaga tcgcgaagat cgtatacacg atcagggcgg
     2341 ctgtgagggg cagacggaac ccgctggaga attgttgttt cgccagggcg agggcgcccg
     2401 tcaggaagag aacactgttg ccgagcgaga cgggtcttga gtaaattccc ccgatgatgt
     2461 tgtctttcgc cgtccaattc gtcatcgcga agccgaggta cgtgccgccg agaagctgta
     2521 tcaggacggg caaaggttcg accgcgggcg agccgacagc ccgcagcatt tccgagggag
     2581 cgaacgacgc ggcaatacct gcgaggccga gagccaggct acttgccacc attaatacct
     2641 tcgtattcac gaattaacct cccctgacga tggaaatcta acaagacgat atggggacga
     2701 cccgcgagtg cgcgcgctgg cccgacgcgg atgcgcatcc ctcccgatag ttctgccggc
     2761 atcatctcaa tcgcatcctc gctcataatc ttctccaatt tcctgtcccc actgcttccg
     2821 cgcgggacgg taaagcttca acctgatgcc cggccctttc ttgagggccg cgccgggccg
     2881 cgcacgacca ccaccgagca atgcgcgttc atcgcgaccg cctcagagac gctccccaac
     2941 aggaatcgct tgaaaccccg ccgcccgtgt gagccgacga cgatcaaatc ggcggcccat
     3001 tttcgcgcct cttctaaaat gacctctttc gcgttatcgg agactgacac cgccatggtc
     3061 actttcaacc ccgcggaagc cagtatctgt tcagcgcttc tgaccgcctc ttgggcctgt
     3121 tcgtcgtgtc tcactctgga aggtttgatc gcccacggct cctcaacgct taggaccctg
     3181 aactcgctcc cttcgggcca gggccgcccg gcgagtgctt gggccgccgc cgccgatgtc
     3241 tccgagccgt cggtggcgag cagcaccctc ctcccgctct ccagagtgtg cgcgcggtct
     3301 tccgccgggt cacgcacgat ctgaacggtg cacggggcgg ccgcgacgac cgctctggcg
     3361 acgctgccaa gcatccagtg tgccaggttc ttgcggacgt gagcgcggac gaagatcaat
     3421 tccgaagacc acttccgcgc gaaaaaaggg atgagttgtc gcgggtcgcc cctcgtcacc
     3481 aacacctctg ccgggatgcc gacctctttc aggcgctcga cggcgatggt ggttatttgc
     3541 tcctccctcc tctccatctc ccgctgcacg gtcgccttcc cgtagccctc ttcacgccac
     3601 acttcaggcg ggacgtccgc gtccacgacg actgaaagta cgtgcgccgt cgtaccgtcg
     3661 ggccacgggc gcactcccac cgcgccgacg agcacttcgg tcgaaaccgc cgaatccgcc
     3721 gcaatcagta gcttcatagt ttaatcccct catcaccacc agcccgtgtt cgggccgggg
     3781 taaatagtca caccgctgaa accgatgcgg gcaaagtccg cccgccgtcc acgcccagtg
     3841 tctgtccgac aatatacttc gcctcgtcgg aagcagggaa caacatcgcg caggcattat
     3901 caccgggttc gcagagtttg ccgatgggaa tggcctgccc catgcgccgc agtgatcacc
     3961 ccctcaccga tgactgaggt cgccggggcc gcgctcacat cgcgcagggt gacctcgggg
     4021 taagtgttaa cacccgagaa cagcaggtgc aaacacgctg gtagggggcg gcgcttcttg
     4081 atagtttacg attaacgaag aaggggaagg tgattttgca taatagtttt cgcagggtga
     4141 ttagatgagg aggcgtaatc agggtatgaa cgtgttgaca acaataacga gagcggaaac
     4201 aacctatgtc gagcaggact ggcggcaggg cgagggcggc agagtattaa cattcgacat
     4261 gatgctcgaa atgccgcatt ccccgaaggt cgccgcgtta ggtcgcagtt ccgagatcgt
     4321 cttcgaggac gaacagtaca tcgtgaatca taaagaagtt gtcagggagg cacctccaga
     4381 gttggttcgg ctcgcgctta acagagccgg gcgcgcggcg gagacttgga gtgcccgaag
     4441 caggcgacac gggtcgcctg cttctttttc tccggtcggg aatgcgccgt ctcttccctt
     4501 tcacagacca gttacaccca ccttttgaac acccgcacgc gagttgccct taacgtgagt
     4561 cttcgtctct cacgcggacg accttcaccg agcagggggc gcggcttgcg acccgttcgg
     4621 agacgcttcc acgtaggagc cgcctgagtc cccgacggtc ttctgtaccg actacgatga
     4681 ggtccgcgcc ccactcatta gcctcgcgtg caatccgtct atcgggtctt cccgcgatga
     4741 cttctgccga cgtcttcacc cccgcgcccg ccaaaatttg tgcggcgtca cctatcgccc
     4801 ggtgcgcccg acgggtcccg ccgccgtcgt acaagccgat ctcttcgatt gaatacgcga
     4861 gcgggttgac catgctcatc accttcactt ccgtcccctc gggccagggt tgctcggcga
     4921 cggcgcgggc cgcgaccgcc gcgtgctcgg agccgtcggt cgcaagcagt atcttcatgt
     4981 ggccgtttgc aacgaccgag cgatcatccc tgaaggcgcg cacgacctcg acggagcagg
     5041 gggcgtcgcg gacgactgat ttagcgacgc tgcccagcat ccaacttcta aagtccgtgc
     5101 ggttatgcgc cctgatgagg atcaggtcgg cgggccagtt cctcgcttct tgcgggataa
     5161 gccactgtgg atcgcctcgc ctgatcgcga cttccgccgt aatgccgagt tcttctaatg
     5221 gctcgacggt gagggctgtg atctgcccct cccttctcct catctcctga tgaacggcat
     5281 ctacacggta gcccgcctcg cgccacacct caggcgggat ggcttcgtct tcgacgaccg
     5341 agagcacacg cgcctttgtc ccgcgcggcc acgggcgcga cgcgacggcg ttcatgagca
     5401 tttcggttgt cacggtcgaa tctgccgcca gtaaaagtct catctcgtca ccaccctcaa
     5461 caaacttatg ggcgcgccgg gtggtagaac tggccgaccg tctcccagcg ctcggtgtcg
     5521 aactggcggc gtgcgccgca ccccatgcac gtccgatagg tctcgccgtc gcgcgtaaac
     5581 gggaggctca acgtccggtg ccagcatccg aaaacccgcg cgactgctct tcccgccctg
     5641 gcgaaggcag acgctaaatc gggagacgga ctcctgccac tctgctcaac tactgcttcc
     5701 atgattactc acctcaagca ttgcttaatc aaaacgcacc cctgaaactt ccatcagcac
     5761 gccggcgcgc tcggcctctg gtcggacgcc ttccagcaga atgcgcacgc ccatcgcctc
     5821 acccggctca agcgccgtct gtcgtgcggg gacgactatc accgtgcgct cgcgcaaagc
     5881 cgatccccgc gcgtccagaa ctgcgcctcg catctccagc ccgctgaggg tgcgccgcgt
     5941 cgtgttcctc acggtcgcgc tcacctctac ggcgaggtcg cccggtggcc gcggcgcttc
     6001 catcgcaacc ggctcctcga cgacgatctt ctcgcggagc tgctcgaact ccggcatctc
     6061 aggccgtagc gccccctcca gtcggggctc agcgatagga cttgcgacgg gccgcgtcag
     6121 agacaagtgc atcaaccccg ccgccactaa cacgagcaaa gttgtgatga tcgctgccac
     6181 gagtattaag ggccaacgca ggtcaggctc tctaatcaat acggggccct gatacgacat
     6241 gattctcctc ctcccgttaa taaaaaatta ccggcgctca cgcgccgcaa cgtctgaccc
     6301 gggccgaacc ctcctttggc gcgaccagaa acctgatgtt gaagccgtga ccgcaatcct
     6361 tgcacacgtg gcggtcgttt tcgcgcgcgc ccacgacgag ccagacttga gcgcattgcg
     6421 ggcactcaac caaccagcgc cttagaatct caggccagct ttcacccagt ctcttgacgg
     6481 gtctgctttc cggcaaaaca acgtgcttgc tctttcttaa ctccatgtgc tgacttaacc
     6541 ccatgtgctg atcattccac ctatgcagtc tgtgatgtgt tggagatcat gtatgcacct
     6601 tagctggttg gccctgcggc agacagcgta ttaacaccta aggggcgcgg gtgttatcac
     6661 cctgcgcccc ttgaagacct cttcatattg gggaaatttt tagagggcgg acgacgacgc
     6721 cgtcggcgtg gcggcgtggc cgagtggcgg ggttcaatcg tgctgcttgt gctccctgac
     6781 gtggagcgca agctcaacgc ggttgctcag actcttttta tcgaggatgc ggctgatgtg
     6841 accctcgacc gtcctcagac tgaggtggag ttcggcggcg atctctttgt ttgaaagccc
     6901 gcgcgcgacc gcctcggcga cgcgccattc cgcatcggtt aaatcttcat cacgcaccag
     6961 ctcaacatcg tccgtctcct gctccccgaa catccgcgca aggtctgagt gagtgcgctg
     7021 cacacggtcg agaatgctct tgacgacggc gaccaactcc tccggctcga acggcttcgt
     7081 gaggtaggcg tccacgccgg tgcggaatcc ggcgatgcgg tcggcggtct cgtctttggc
     7141 cgttaggaag acgatgggga cgagacgcgt gcgcggggcg gagcggaggt tacgggcgag
     7201 cgcgtagccg tccatgcccg gcatgcggat gtcgctgatg ataaggtcgg gcagcgcctc
     7261 ggcaacgcgc accatagctt ccgcgccccg acgggccgtc accacctcgt agccttcagc
     7321 ccgcagcgtc tccgagaccg cgagcaggag gctcggatca tcgtcaacga ccagtaactg
     7381 ttttgacatt tctatcctcc acttcgggct gcttccggca tcccccgtca cgccataccg
     7441 gcagatggag cgtgaacgtg ctcccgcgcc cgacttccga ttcgacgctg atcttgcctc
     7501 ccatctgctc catcacgttg cggcccaaat atagcccgag gccgacgccg gacacgtcag
     7561 cgtcctccag aaactcggcg tccgtcgtgg cgtttcgcat ggcctcggag tgcgtcgccg
     7621 ggcgaccgcg atgaaacttg tcgaagagaa ccggcatgtc ctcaggaggg atgccgcgcc
     7681 cgttatccgt cacgctgatc agaacctcgt cgccgtctgc gcgagccgac agctggatgc
     7741 gcccgccgtc aggtgtgtac ttgatggcgt tctcgatgat gttcgacagc acccggcgga
     7801 gcgccttcgg gtcggcgcac acgtttgaaa tgtcttcgac aggttcgacg cggagttcat
     7861 ggctcctctt ctccgccgcc cttgcctccg acttgacgac ggacgagacg agttccgcga
     7921 catccacgcg ctcgtgcgtg atgcggaaga ctcctccctc gaggcgcgac aggtcaagca
     7981 ggttaagcac cagatcaatc tgccggtcgc attcgactga aattgtatcc aggtactcgc
     8041 gccgcttcgc gtcatccaac ccgtcgcgca tcaggagtct cgtcagcgcc ttgatcgtcg
     8101 tcagaggcgt gcgcagctcg tgtgagacgc ttgagacgaa gtccgacttc aagctgtcta
     8161 attcagccat gcgcgcctct ctctgctcca gccgctcagc catcgcgttg aagctcactc
     8221 cgaggcgtga aatctcgtcg gcgccagcgt cgggcgctcg cgccgagaag ttcccctccc
     8281 cgaaccgctg cgcggcgaaa ccgaggaggc gcacgggccg ggcgatgctc cgcgcgacga
     8341 ggagcgcagc cgtcaaagtg ccgaagacga cggccaaact gaccagcgcg taagtggtga
     8401 gctgacgcct cgcgggcgcg tagagggccg cgctcggtac gccgaccatc gtcacgtagc
     8461 ccgtgcggcc tgcgtgcgcc agtccgtacg tgcgctcgac gccatcgatg gtgctcgtga
     8521 cggtgacgac cgcagtcgcc cggtcgccga gcgcggataa cactccggcg tcgctcagat
     8581 caaggccgag agcggattcc ggcgtctggc tgcggtagac gacgcggcgg tgcgaatcga
     8641 gcagggtgat gacgagtgcg cgttccggta aggcgatgcc gtggaagagg tctccaagag
     8701 ccttaccgtc aacacgtgcg atacaagcgc cgccgccctc cattggagcc gcgacggcga
     8761 gaacataccg gccctctcca cgcgtccagt ccgtttcgat ggcggcctcc ccgccgtgcg
     8821 cctccgcgat cagcctctct gcgacgcctg ccggaagact ttcagcgtcc gaggggaacg
     8881 ccgccacgac ccttccgccc gcatcgaggg cgcgcacgtc aatccagtgc gggcgccggg
     8941 ccgtcgccgc gcgcaagtcg gcttcgagcg ccgccgcgtc cgtgaagtgt ccggcggggt
     9001 aagaggcgat tgtgatgagc ggttgccgct gagcgtcgag ccagcggtcg aagacgacgg
     9061 ccgccaactc cgcctgttgt tccagagact cgttgagctg ttgttctttc gcgccccaca
     9121 ccacccacag catcgcgagg ccgaggagga cgagcgggac ggccgcgccc agcgccagtg
     9181 caagtaatcg cccgcggatg ctcaaccgtc gcctccaagt cctcagctca gtcctggtct
     9241 attttcttct cagtataaca aatcgtccag acttccatga caagagaaaa cacctgcgtt
     9301 cggtacgtga taacactctc gcccttgacc gacgaactaa ctacgattgg caaatcaaac
     9361 gcgcgcccat catgaacgat acgggtgtgg tgattatgga ctggggtgtc aatagtaggg
     9421 ccgtaagagg gaaggcaatc atggaccatc tcaggaacgg gcggcagtct tcattcgggg
     9481 aatcttcgcg cgcggctgag ttgcccggct ctcgccgcgc agatcggtgg gcggtgattt
     9541 tggcgggcgg ggacggcaca cgccttcgac cgttgacacg cttgatcgcg ggcgacgagc
     9601 gccccaagca gttctgcccc gtgatcggcg atcaaacact cctcgaccag acgaggaacc
     9661 gtgtcgcgct ggcggtccga ccggaccaga cgatattcgt cgtcacgcaa catcacgcgc
     9721 gtttctacaa gccgcttctg ggcgatgtgc ggcggcagca gttggtggcc caacctaaga
     9781 acgtgggaac ggcatcggcc attctctacg gtttgctacg cctctcacag gttgccccgt
     9841 cgtcctctgt agccttcttc ccgtcggatc actatttctc cgatgacgac cgcttcatgt
     9901 cccacgtcga atccgctttc gaggcggcgc aggtgtgtga ggacctggta attctgctgg
     9961 gcatccagcc ggacggcccg gaggtagagt atggatggat cgagccggct tcgccgggcc
    10021 tgatgaagaa cccggacgcg ctttgtcggg ttcaccgctt ctgggagaag ccgactacgc
    10081 acatggccca cgcgctcatg gacatgggtt gcctgtggaa cagttttgtg atggtgggcc
    10141 gggtttccgc tttcctggaa atgatccgcc gggctgcgcc ggaactctac gggcgcttcg
    10201 acgcagtcag gccagtgctt aataccgctg acgagggaag ggcgatagac gggctgtacg
    10261 ctcaactccg tgagactaac ttctcacaac aggtgctggc cgcgcgtccc gaagacttgg
    10321 ccgtacttcc cgtcagcggg ttgagatgga gcgacttggg gcagcccggt cgcgtcttgt
    10381 ccacgatggc cgacattgga cttgagccct ctcgcggagc ggttctagga tttagtcacg
    10441 ccgccgaaag ccgcggccag agggtgaatc tgttgaccag ggatcgggag gcgctcacga
    10501 ggtgatattt cggacaggcg ggacattcta tcgcggggcg tctgccgtcg agatcgcgcg
    10561 cgcgcttgag cgcgacgcgg cggattaccc tcgccccgga caaacgcttc gacaattcct
    10621 gctctggtcg ctcaaccact tgctagaccg cgtgccgccg cgcgaactgg atttgagcga
    10681 caggctggat gatgaggcgc tcgcgttcag ctacctttgt ttatgcgacg agtacgacgc
    10741 gggcgagtta acagttgagc cgtagggacc attgaattga ctatcgccca ccctctcacg
    10801 ctccgaccat aaaaatataa ataaggcctc atgctacata gagaattctg ccagggcggc
    10861 gcgtgtaaac acgtgggatt ttcgggtgtt aacaccctgg cagctgtcag gctcgtcagc
    10921 taatgtagtt tcctgcgcct gatttactgg tgcgcggtca caagacggat taagcttccg
    10981 cgcaaaggag tcaacagatg aacaccaccc aaccccatcc aagttttttc gacaatgttt
    11041 ctctctattt cgaccatgcg gcttctttca cacaacaccc tgaggggctg ctcgatcaga
    11101 tcaaggtctg caacagcgtt tactcattcc agtttcctgt tcgcacacag cgcggcatcg
    11161 aggtcatcac gggctggcgt gcccagcaca gccaccacaa gctgcccgtc aaaggcggga
    11221 tcaggtattc ggaggacgcc aacgaggacg aggtgaaggc gctggccgca ttgatgacct
    11281 acaagtgcgc cgtgatggac gtgccgttcg ggggcggaaa gggcgctgtc aagatcaacc
    11341 cgaagaacta cacggtcgag gagttggagc agatcacgcg ccgttacacc tccgagctga
    11401 ttaagaagaa cttcatcggc cccggcgttg acgtgcccgc acctgactac gggaccgggc
    11461 agcgcgagat gtcttggatc gttgacacct acgccgcgtt ccatcccggt cagatagacg
    11521 cgctcgcgtg cgtcacagga aagcccgtgt cgcagggcgg cgtgcgcggg aggctcgaag
    11581 ctacggggcg cggcgtcttc ttcggcctgc gcgaggcgtg cggcgacggt gaagacatga
    11641 aacggctcgg cctcgacagg gggttggagg gcaagcgcgt cgtcgtgcaa gggctgggga
    11701 acgtcggcta tcacgcggcg caattctgcc agcagggcgg agcggtcatc acggggctcg
    11761 cggagtacga gggcgcaatc tacagcgcga aaggcttgga cttagaggcc gtgatgaagc
    11821 accggaagga gaccggctcg ctcctcaact ttccgggagc gagcaacctc ggcagccgtg
    11881 aggaggcgct tgagcttgag tgcgacatct tgattccggc ggcgctcgaa aaccaactca
    11941 cggaggagaa cgcggggcgc gtcaaggcga agatactggc cgaggcggcc aacgggccga
    12001 ccagcgccgg cgccgaagag attctcctgc gcaagggagt gctggtcatc ccggacattt
    12061 atctgaacgc cggcggagtc accgcctcgt acttcgagtg gctgaagaac ctctcgcacg
    12121 tccgcttcgg caggatgggc aagcggttcg accagacgac tttcgagaac atgctgcggg
    12181 tggtggaaac gactacgggg aagaagctct ccgacgagga gatcaagagg atcgcgcgcg
    12241 gcgcggacga gatcgacctg gtcaattcgg gtcttgagga gacgatggtg gtcgcctacc
    12301 accagattca tgagatatgg aaagcggact cgaagattcc gggcctgagg accgcggctt
    12361 tcatcagcgc gataaataag atcgccacct cttacgccga gttgggcatt ttcccgtgag
    12421 cgctgaaagc gggacgtagc ggtccgatgc tcaggcagca tcggggcaat agagttaata
    12481 agaacaacgc ggggtgaatt cgatgatgaa aagcggtgag cacaaaatag caacggcaga
    12541 tgccggcaac gggcagtatc cggtcgagga cgtggaaagc tccgaaccgt tagaactgga
    12601 ggtcgaacgc ttacaagagg agttggcggc cgagcgcgag cggggcctgc gtgcgctggc
    12661 ggaatttgac aactaccgcc ggcgggtgag gcgcgagagc gccagcgcag aagaagccgg
    12721 aaagcgcgac atcatcctcg cgctactcga tgtgatggac gacttcgacc gcgccctcgc
    12781 gcacgtcagc gatgctcccg acgcggtggc cgacgggttg aggctgattc accagcgttt
    12841 gagccgcgta ctcgactcaa acggggtcac tcctttccaa agcgcaggag agcagttcga
    12901 tccgacggtg cacgaggcga taagcgtggt cgagagcgac ggacacgagt ccgacacggt
    12961 gtacgaggaa gagcgtcgcg gctacctctg gaacggcgaa ttgctccgcc ctgcgcgcgt
    13021 catcgtcgtc aggtgagagc acgtctcagc tcacaaaggc tttgagtcaa cagtgacagc
    13081 tgatgtaaaa gtagacgcac ttgatgccgg aggtcggcga tcagcgatga gcggcggggc
    13141 acaggtgaag agtgacgatc tgccagcgcc cctgccgcct cgcgtacatg cggaaaaatt
    13201 tgtaggtgcc cgaaaaatct cgatctctgt actgccaatt cgccgtttca cttccaaaga
    13261 tcacacccgc ccctccgtag acgcgcacag tagcgtcgtg gggttcgagc gattccaccc
    13321 tgacctcacc cgctacaatg tcggcgataa actgctcctt atcatcgccc tcgaacggat
    13381 acgcgaacat gaagtcgtcc gccatgatac ggtcaagcgc tgctctgtcg cgctgcgcta
    13441 aagccttggc ccattcgtca ttcacccgcc ttagttcctg ttcaacttgg ggatcggtcg
    13501 ctgcttggcg gccttcatct gggctaacca tgagcgcgcc tcctttcggg gaactgtgtc
    13561 gcagtgcctt ttataccaac cgacagggca aatcaataag tacatgtgcg agcatgggca
    13621 ctggggtgac ttttgcatca gaaccatcac ctgattcaaa agtcgtgcaa gtcttgcact
    13681 tttgcatcaa agcactcaca aaggttaaag cggcagaatc aaattgaact ctcagtagcg
    13741 atttcactca gcgacccgtg agcaactcat aataagcggt gatgccgttg aggatgaact
    13801 gcacggcgag ggccgccagg atgatgccca tgacgcgcgt gacgatgtgc actttgcctt
    13861 cgccgatgcg cgccatcaaa ctcccgccga gccgcagcat gatgagcgag accccgtaca
    13921 caagaacaac tgctaaacct aacgcgacga ggcggcgggt gtccccgttc gcttgggcgg
    13981 tgagcaacag gatggtcgca atcgcgccgg gtcccgatag aagcggaatc gccagtggaa
    14041 agatcgcaat gtcttctccg gccgtcgggc gctcctctcg ttccggcgct tggagtccgc
    14101 cgcgatgacc gaagagcatc ggcagggcgg ttgcaaacag cagcacgccg ccgctgatcg
    14161 caaaggcgtg aatcgtcacg cccaaatatg aaagcaagct gcgcccggcg acgaggaaaa
    14221 acagcgcgac gaggagggcg atgatcaccg cccgcttcac ggtcgcgcgc cgtttcgcgg
    14281 cgtccgcccc ttcggtgagc gcgacgaaga tcggcacaac tccgggcggg tcaaccacca
    14341 cgaggagtgt gacgaaagcc gtcagaccga accgtaccag cccttcgtcc atcagacctc
    14401 aacacaaggt cgcaaggttt acgcgcatcg taacccgagc gccgcggact ctgcacgccg
    14461 catcttcagc gctgctcatt gccgggtagg ctttcgcttc tttgtgggag aggcgggagt
    14521 atcgaccgcc gagttatcct gcgaagctga tagttgttta ccctcgccct catggccgct
    14581 cgccggtgtg ctctcatcca cacctgtcgg gtcggcggaa atcctcccgc cctcgttctc
    14641 ccaccggtca agtaccttct tcttcagacg ctttctggca atcggatccg gcatcgccgt
    14701 gttctccttt cccaactcat tgtctcataa ttctcccggc gtttcgctgt gcgggcgggc
    14761 gttactcact catcgccgcg cgcctgctca ccgtccgacg tgcggacgtg gttaagtcat
    14821 cgggttgttg cggctgcgcg tcttgccggg cggggcgatt gcttttccgt gaacgcattt
    14881 caagccacgc cacgccgccg aaaaagatga gtatgccgag tgcgacaacg ataattgcgc
    14941 ttagatccat gattgcctcc ttcaataatt tatggtgtct ccttacaacg ccccgttcgt
    15001 cgcgagcgtt tcttttgctc gttagcaact tagccgaaag ataagacagg agatagcgtg
    15061 aaaacacctg tccttgatgg gtgatgacac ggctgctcgc cggctttacg atggtcgtac
    15121 tcgtcaccag taaccgctca cttccttcaa tcaataattc gggtgttaac acctacggtt
    15181 tttaggtgtt aacacgctgc cccgaatcaa aggccgtcgt ctaatattta gacattcatt
    15241 gatcgtcgcg cgcgggcttc ggaggcggtg cgaccgtagg aggcggcagg cccggggggg
    15301 acgggccggg tcgccgcggg cggccaccgc agggcagggc g
//
LOCUS       DASVES010000314        11033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1210387, whole genome shotgun sequence.
ACCESSION   DASVES010000314 DASVES010000000
VERSION     DASVES010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210387"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..3538)
                     /locus_tag="VF240_12740"
     CDS             complement(116..3538)
                     /locus_tag="VF240_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7176120.1"
                     /translation="MKSLLPHSHRSGSSARGYVALALCAMLLTSTVSPAFAQRRTSIR
                     TATGEAKQPTAAPASRKSEREKQGAARARLKAGAQQEGIRLGRPAPAGQDGERIGVPW
                     VGEAGLQKTSDEIMTEQSVADTRPAKTPRVGKGIKHKEKIDKTGRPQDPNAPAVASWP
                     FISAAPPKGPAVRTRAKSGEGEEAAAAPAPLAPQTIGMNFDTVTGPTETGAFPPDTMG
                     AIGPAQFFVFLNGRLRTFNKTTGAADGVINVDSDVFFASVMTPAPPPLAINFTSDPQV
                     RYDRLTGRWFLLIIDVPSANDIGDTPNRILLAVSDAASAGVISAGTVWTFYFVQQNTV
                     GGGDSGGFCDYPSLGVDANALYTGCDEFGAASGGFNNTTAFVIRKSSVLSGGPVVTTA
                     FRDLIGTDGPFEPRGVDNYDPAATEGYYIGASAAAFGRLIMRRIATPGTTPTISANIS
                     ITVPATSFPRSVDHLGDSGGTGTTGNLDALDDRLMAAHIRAGRLWTAHAISVLATGVA
                     SGTSAQRRDAVRWYELIVPPTTGTPTVNQSGTIFDSAATLAAARQYFIPSIMVSGQGH
                     AALGYSTSGTPFRADAATNGRLVGDTLGTLGAVNIYTASTTAYNPPGDPGSPRRWGDY
                     SFTSLDPLDDMTMWTVQEYCNGTGTYGIRVAKLNAPPPATPTAGVPLSVPQNTPSTNV
                     VITGTQVSGSGFYDPGANLAPPALPFNHISASVTGGVTVNSTTYNSPTQVTLNVSTVG
                     ASLGSQTVTITNPDGQSLSGTATITVTPPLAPAATAGQVLINEFRFRGAGDAADEFVE
                     LYNNTNGPLDISGYTLHALTAAGAQNLRFTVPGALASNTTVIPGRGHYLITGASYSLA
                     AVAASNGTLSTGIVDGSSIGFFAGATPTAGTRIDSAGFDNRDALFFEGTVITPGGAGT
                     GGITVNGEYSFVRKVPTLTRLPQDTGDNNADFTFVSTTAGTFSTRVSALGAPGPQNLA
                     SPVFKSFSQFIETLLDPTQGSTLVPNRVRTSRASCPGCDNTKSNLGTVQFRRKYTNNT
                     GGPITRLRFRISEITTLNNRLATEADYRALHNTGNILVSTSGGNVTVESLQLEPPSDA
                     VTNGGALNSTLSAGTITVGTPLAHGAHINVNFLFGVQQIGDYRVFITIEALP"
     gene            complement(3901..4461)
                     /locus_tag="VF240_12745"
     CDS             complement(3901..4461)
                     /locus_tag="VF240_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176121.1"
                     /translation="MFRVGNAPHALACLPLLLAAALQSGSCRAGDVVPGGGAAGVANG
                     NVSEQRARPAANRDDNANPKAKMEDSQERKDARASDDTWGGDHIRLVVREGGADIEYD
                     CAHGTMDAPLDPDDAGRFDVAGHHVREGPGPIRLGKAPPSRPARYTGQVSGPTMMLTV
                     TLTDTSQEVGVFTLTRGREGRLRKCR"
     gene            4626..5489
                     /gene="ttcA"
                     /locus_tag="VF240_12750"
     CDS             4626..5489
                     /gene="ttcA"
                     /locus_tag="VF240_12750"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX7176122.1"
                     /translation="MFVESKTKTKLLRQMGEAIKDFGMIEEGDRVMVCLSGGKDSHAL
                     LDLLLDVQKRAPVSFEIFAVNLDQKQPNFPAHVLPEYLEGRGVPFRIVERDTYSVVKR
                     LVPEGKTYCSVCARLRRGILYQTAVEEGCTKVALGHHADDIITTFLLNLFFVGQLKAM
                     PPVLRSDDNRNTIIRPLAYCREADLTEYAAQKEFPIIPCDLCGSQSDLKRARMKRLIS
                     ELEREIPHVRASMLTALTNITPSHLLDPNWFDFKNLRAATGDVEAELDIAIGHADPSH
                     GELRPALVSIG"
     gene            5508..6407
                     /locus_tag="VF240_12755"
     CDS             5508..6407
                     /locus_tag="VF240_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176123.1"
                     /translation="MATAATKHKELDWGAFPAGAVIEYETQLCLACIFNIFTKQFGVA
                     PRTAYTEIKRHSPSVEELTTREPARPYFDSAEKHPHCPHCDSTKRWHARLTTYRIEGG
                     KTTDAARRALVKKLPKSEGQFEVMEKKATAKAVFFEWLDDLGRALDFEDDRSWMLEAT
                     RAYLQRREPKTDWAEVFDGVRQVRRSQRLEEGWERDGNRLFISPPLYNDVLLVQYLVS
                     RSHRHGGRTFEGRLTLVELVRRLRYSGHLEAHGITDRDQFEVLEQLVEQLTGGDAAVK
                     LHYVIDRRDLLEKTKDVYARYAG"
     gene            complement(6514..7680)
                     /locus_tag="VF240_12760"
     CDS             complement(6514..7680)
                     /locus_tag="VF240_12760"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEX7176124.1"
                     /translation="MKSSFVSTRGWLALCLSVLMLAGAAAALPTGDGPPKKSGWQTQS
                     VGRPISYKNLTLFPVYSRGATADADAYITLDEGIRAGTVIITERGGGDTAATLQRRPS
                     GRGSAAVQQASMRSDNASVNELSLTNRSGRKLMLLAGEVIVGGKQDRIVQDDVVIPPV
                     SVPISLSVFCVEHGRWSQRTSAEASGGGGGGAAPAPVIAPSGARKSENFYSLGAVAHP
                     TLRAAAQDKKQQSEVWKEVGENNAKLGTGSGTDTYQQVYASKKVADSVESYVGALRGR
                     LLGPGVVGVVVARNGEVVWADVFASPGLFARYWPKLLKSYAVEALAEQRSERSPTADE
                     ASRYLYDRAGTRQHSGSAGVFELVKIENPRHAIFELWDISLAEPLRLHFNKVRR"
     gene            complement(8030..9757)
                     /locus_tag="VF240_12765"
     CDS             complement(8030..9757)
                     /locus_tag="VF240_12765"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7176125.1"
                     /translation="MRVTIGQINTTNGDYEGNTEKILRAIEQAKSEKSDLIVFPEVCV
                     QGYTSLDWFLDADIARSALKPLERIIPAAEGITAVVGTVRPTGQTSGRKLFNSAAVIS
                     NGQLLGFADKTLLPVYDVFDDPRYFESATERQLFEAGGERLGVPVCEDFWNDKTFWRE
                     RLYAHDPTDELIEMGASLIVSPNASPFNKSKMGERCKMVSHRSKASGLPIVYVNLVGG
                     NDGIIFDGASLVTDCRGETILQAPPFEEFIGTIELDCGTADDRCLPGDDIETVRSALV
                     LGLRDYAHKNRFRRALLGLSGGIDSAVVAALACEALGAENVLGVMMPSPFSSRGSVED
                     SVAFGKSLGMPVIKHPIIDAFEVLTKQLGLTRPTRGGESLAAENLQSRLRGNILMTIS
                     NAEGRLLLSTGNKSELALGYCTLYGDTNGGLAVIGDVLKTEVYRLARHINREREIIPW
                     AIINKRPSAELAPDQFDDQSLPAYDVLDPILQMYFERKATPEQIIAEGSDAALTYEVL
                     NRVESPANEFKRRQLPPTLIISRNAIGVGRRRPVTHRYRRQPETPAERAAVQEAPHEL
                     PEGRAAEPV"
     gene            complement(9812..10021)
                     /locus_tag="VF240_12770"
     CDS             complement(9812..10021)
                     /locus_tag="VF240_12770"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX7176126.1"
                     /translation="MRYAMIIEKGERNYSAYLPDLPGCIATGKSVAEIKQRMREAVEL
                     HLRGLREDGLPVPEPTSMAEYVEAA"
     gene            complement(10036..10209)
                     /locus_tag="VF240_12775"
     CDS             complement(10036..10209)
                     /locus_tag="VF240_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX7176127.1"
                     /translation="MIKLIEKDGWYHVVTEGSHRQYKHPARPGRVTISGHPRDDIHPK
                     TLKSILTQAGIKE"
     gene            complement(10494..>11033)
                     /locus_tag="VF240_12780"
     CDS             complement(10494..>11033)
                     /locus_tag="VF240_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176128.1"
                     /translation="VFNDRCRDVRRQGRAGALALVWCEALVDLAKTAAAERAALWAGG
                     GMMRILRTVVLALAAYAFALLVAAPLYMRNRESLPSFVGNMADALIATGVVFNVVFQI
                     LTLPRFVEGVRAVRLAGLLTALLIGGLIVFIAMTGGPPARPHLSVIVAQVLSFFIWYG
                     AHLWWVLRRRDASAPPEPA"
BASE COUNT         1762 a         3479 c         3831 g         1961 t
ORIGIN      
        1 gccgcgtgga aactccttcg taaaaaagcg gaaggcgcgc cgctcaccct cgcgaggggt
       61 gtgcggcgcg ccttccggcg acggccgcgt cggctagcga cgccgcggcc tttttttacg
      121 gaagcgcctc gatggtgatg aagacgcgat agtcgcctat ctgttggacg ccgaacagga
      181 agttgacgtt gatgtgagcc ccgtgcgcga gcggggtgcc caccgtgatg gtgccggccg
      241 agagcgtcga gttgagagcg ccgccgttgg tcacggcgtc ggacggcggt tcgagctgaa
      301 ggctctcgac cgtgacattc ccgccgctgg tgctcaccag tatatttccg gtgttgtgta
      361 acgcgcggta gtcggcctcg gtggcgaggc ggttgttgag cgtcgtgatc tcgctgatgc
      421 ggaaacgcag gcgcgtgatg gggccgcccg tgttgttggt gtacttcctc ctgaactgca
      481 ccgtgccgag attggacttc gtgttgtcgc acccagggca cgacgcgcgg gacgtgcgga
      541 cgcgattggg cacgagcgtc gagccctgcg tcgggtcgag gagcgtctcg atgaattgac
      601 tgaacgactt gaagacggga ctcgcgaggt tctgcggccc cggcgcgccg agggcggaga
      661 cgcgggtgct gaacgtcccg gcggtcgtgg agacgaaggt gaagtcggcg ttgttgtcgc
      721 cggtgtcctg cggcagtctg gtgagcgtcg ggaccttacg cacgaagctg tactcgccgt
      781 tgacggtgat gccgccggtg cccgcgccgc ccggcgtgat gaccgtgccc tcgaagaaga
      841 gggcgtcgcg gttgtcgaag ccggcggagt cgatgcgtgt cccggccgtc ggggtcgccc
      901 cggcgaagaa gccgatgctg gagccgtcaa cgatgcccgt gctcaatgtc ccgtttgaag
      961 cggcgaccgc ggcgagactg tagctcgcgc cggtgatgag atagtggccg cgccccggaa
     1021 tgaccgtggt gttgctggcg agcgcgccgg ggacggtgaa tctcaaattc tgagcgccgg
     1081 cggcggtcag cgcgtggagc gtgtagccgc tgatgtcgag cggcccgttc gtgttgttgt
     1141 acaactcgac gaactcgtcg gccgcgtcgc ccgcgccgcg gaagcggaac tcgttgatga
     1201 gcacctgtcc ggcggtcgcg gcgggcgcga gcggcggggt gacggtgatg gtggccgtac
     1261 ccgaaaggct ctgcccgtcg gggttggtga tggtgacggt ctgcgagccg agcgacgcgc
     1321 cgacggtcga gacgttgagc gtcacctgtg tcgggctgtt gtaggtggtg ctgttgaccg
     1381 tcacgccgcc cgtgacgctg gcgctgatgt ggttgaaggg cagcgcgggc ggcgcgaggt
     1441 ttgcgcccgg atcgtagaaa ccggagccgg agacctgcgt gccggtaatc accacgttgg
     1501 ttgaaggcgt attctggggg acgcttaagg ggacgccggc ggtcggcgtc gccgggggcg
     1561 gcgcgttgag cttggcgacg cgaatgccgt aggtgcccgt cccgttgcag tactcctgaa
     1621 ccgtccacat cgtcatgtcg tcgagcgggt cgaggctggt gaaggagtag tcgccccagc
     1681 ggcgcgggct gcccggatcg cccggcgggt tataggccgt ggtgctcgcc gtgtagatgt
     1741 tcaccgcgcc gagtgtgccg agggtgtcgc cgacgagccg gccgttggtt gcggcgtcgg
     1801 cgcggaaggg cgtgccggag gtgctgtagc cgagcgcggc gtggccctgc ccggagacca
     1861 tgatggacgg aataaagtat tggcgcgcgg cggcgagcgt cgcggcggag tcgaagatcg
     1921 tgcccgactg gttgacggtc ggtgtgccgg tcgtcggcgg cacgatcaac tcataccagc
     1981 ggacggcgtc gcgccgttgc gcgctggtgc cggatgcgac gcccgtagca agtaccgaga
     2041 tggcgtgcgc tgtccacagg cggcccgcac ggatgtgcgc ggccatgagg cggtcgtcga
     2101 gcgcgtcgag gttgccggtc gtgcccgtgc cgccggagtc gccgaggtga tcgacgcttc
     2161 tggggaagga ggtcgcgggg actgtgatgg agatgttggc ggagatggtg ggcgtcgtgc
     2221 ccggcgtggc gatgcggcgc atgatgagcc ggccgaaggc ggcggcgctg gcgccgatgt
     2281 agtagccttc ggtggccgcc gggtcgtagt tgtccacgcc gcgcggctcg aacggcccgt
     2341 ccgtgccgat gaggtcgcgg aaggcggtcg tgactacggg gccgccgctc aggacggaac
     2401 tcttgcggat gacgaaggcc gtggtgttgt tgaaaccgcc ggacgcggcg ccgaactcgt
     2461 cacagcccgt ataaagggcg ttggcgtcaa cgccgagcga cgggtagtcg caaaaaccgc
     2521 ccgagtcgcc gccgccgacc gtgttctgct ggacgaagta gaacgtccag accgtgccgg
     2581 ccgagatgac gccggcgctg gccgcgtcgg agacggcgag gaggatgcgg ttcggggtgt
     2641 cgccgatgtc gttggcgctc ggcacgtcga tgatgagcag aaaccagcgt cccgtcaggc
     2701 ggtcgtagcg cacctgcggg tcgctcgtga aattgatggc gagcggcggc ggcgcgggcg
     2761 tcatcacgga ggcgaagaaa acgtcggagt caacgttgat gacgccgtcg gccgcgccgg
     2821 tcgttttgtt gaaagtccga agccgcccat tgaggaagac gaagaactgc gcggggccga
     2881 tggcgcccat cgtgtcgggc gggaacgcgc cggtctcggt cgggccggtc acggtgtcga
     2941 agttcatgcc gatggtttgc ggcgcgaggg gggccggcgc ggccgcggcc tcctcgccct
     3001 caccactttt agctcgcgtt ctgacggccg ggcctttcgg cggggcggcc gagatgaagg
     3061 gccaactcgc cacggctggc gcgttcgggt cttgcgggcg tccggtcttg tcaatctttt
     3121 ccttgtgctt gatacctttg ccgactctcg gcgtcttggc cggtctggtg tcggcgacgg
     3181 actgctccgt catgatctcg tcggaggtct tctgcaatcc ggcttcgcct acccacggca
     3241 cgccgattct ctcgccgtcc tgacccgcgg gggccgggcg gccgaggcgg atgccctcct
     3301 gctgcgcccc ggcctttaaa cgggcgcggg cggccccttg cttctcccgc tcactcttcc
     3361 gagacgcggg ggcggccgtg ggctgttttg cctcgccggt ggcggtgcgg atggaggtgc
     3421 gcctctgggc gaaagcgggc gagacggtcg aggtcagcaa catcgcgcac aacgccagcg
     3481 cgacatatcc gcgcgcggac gaacctgacc tgtgggagtg ggggagcaag cttttcataa
     3541 atctcctttt gattcctccg gctatgtaat ggctcgcgtg tgtgagccgt tcggtccttg
     3601 gcgctgacaa ggaaagttag ggatgactcg atagcgcgga aagtgatggg cgagaaactg
     3661 ttgaggatag gcaagaagga aacggcggtc ggagcgaaat cttatgacgc gaagccagcc
     3721 gtctgcggcg ctccggcaaa aatggcgcga acgaagatta ccttcagctc tcgcgtcgtg
     3781 tcaatttaat gtcgtattga tggcggattt aattcccaca ctgattcgag gcttcgggca
     3841 cgaccccgta cagcgcgatg cctgccgcca gtgagcatcc caccggccgc gagggcttgg
     3901 tcagcgacac ttcctgaggc gcccctctcg gccccgcgtc agcgtgaaga cgccgacctc
     3961 ctgcgacgta tcggtgaggg tgacggtgag catcatcgtc ggcccgctga cctgtccggt
     4021 gtagcgcgcc gggcggctcg ggggagcctt ccccaagcgg atggggccgg ggccttccct
     4081 gacgtggtgg ccggccacgt cgaagcgccc ggcgtcgtcc gggtcgagcg gcgcgtccat
     4141 cgtcccgtgc gcgcagtcgt attcgatgtc cgcgccgccc tcgcgcacca cgaggcggat
     4201 gtgatcgccg ccccacgtgt cgtccgaggc gcgcgcgtcc tttctttcct gtgagtcttc
     4261 cattttcgct ttcgggttgg cgttgtcgtc gcggttggcc gcgggccgcg cgcgttgctc
     4321 cgaaacgttg ccgttggcga cgccggccgc gccgccgccg ggaacaacgt cgccggcgcg
     4381 gcaggagccg ctttgcaggg cggcggccag cagcagcgga agacaggcga gcgcgtgggg
     4441 cgcgtttccg acccggaaca tgagcgcaag tatctcacaa acggggcgcg ccggtgacgc
     4501 cgggttgcat ctggtcggtg gatgacttaa aacataaacg gtctgggcag cgagtccgca
     4561 accaactcca agcgttgtgg gcctgcgact ccagacccaa attttcttta tcatgggttt
     4621 acaagatgtt cgtcgaaagt aagaccaaga ccaagctgct gcggcagatg ggcgaggcca
     4681 tcaaagactt cgggatgatc gaagagggcg accgcgtgat ggtctgtctt tcgggcggca
     4741 aggacagcca cgcgttgctc gacctcctgc tcgacgtgca gaagcgcgcg cccgtctcct
     4801 tcgagatttt cgccgtcaac ctcgaccaga aacagccgaa ctttcccgcg cacgtgctcc
     4861 ctgaatacct ggaagggcgc ggcgtcccct tccgcatcgt cgagcgcgac acctactcgg
     4921 tcgtcaagcg gctcgtcccc gaaggcaaga cctactgctc ggtgtgcgcc cgcctgcgcc
     4981 gcggcatcct ctaccagacg gccgtcgagg aaggctgcac gaaggtcgcg ctcggccacc
     5041 acgccgacga catcatcacg acgtttctcc tcaatctctt cttcgtcgga cagctcaagg
     5101 cgatgccgcc cgtgctgcgc tccgacgaca atcggaacac catcatccgc ccgctcgctt
     5161 actgccgcga ggccgacctc acggagtacg ccgcgcagaa agagttcccc atcatcccct
     5221 gcgacctctg cggctcgcag tctgatctca agcgcgcgcg catgaagcgg ctcatctcgg
     5281 agctggagcg cgagattccc cacgtccgcg cctcgatgct cacggcgctg accaacatca
     5341 cgccctcgca cctgctcgac ccgaactggt tcgacttcaa gaatctgcgc gcggccaccg
     5401 gcgacgtgga ggccgaactc gacatcgcca tcgggcacgc cgaccccagc cacggagagc
     5461 ttcggccggc gctcgtctca atcggctagg gggacgggtg ggcgacgatg gcgacggccg
     5521 cgaccaaaca caaagaactg gactggggag ccttccccgc gggcgccgtc atcgagtatg
     5581 agacgcagct ctgtctcgcc tgcatcttca acatcttcac caagcagttc ggcgtcgccc
     5641 cgcgcacggc ctacacggag atcaaacgtc acagcccgtc ggtcgaggag ttgacgacgc
     5701 gcgagccggc gcgcccctac ttcgactccg cggagaagca tccgcactgt ccacactgcg
     5761 actctacgaa gcgttggcac gcgcgcctga cgacctaccg catcgaaggc ggtaagacga
     5821 cggacgccgc gcgccgcgcg ctcgtcaaga agctgccgaa atcagaagga cagtttgagg
     5881 tcatggagaa gaaggcgacg gcgaaggctg tcttcttcga gtggctcgac gacttgggcc
     5941 gcgcgctgga ctttgaggac gaccgctcat ggatgctgga agcgacgcgc gcctacctcc
     6001 agcggcgcga gccgaagacc gactgggccg aagtcttcga cggcgtcagg caggtgcgcc
     6061 gctcgcagcg tctcgaagag ggctgggagc gcgacggcaa tcgtctcttc atctcgccgc
     6121 cgctctacaa cgacgtgctg ctcgtccagt atctcgtcag ccgctcgcac cgccacggcg
     6181 ggcgcacctt cgagggccgt ctcaccctcg tcgaactcgt ccgccgcctc cgctacagcg
     6241 gccacctcga agcccacggc atcaccgacc gcgaccagtt cgaagtcctc gaacaactcg
     6301 tcgaacaact caccggcggc gacgcggccg tcaaactcca ctacgtcatc gaccgccgcg
     6361 acctcctcga aaaaaccaag gacgtttacg cccgctacgc gggctgaagg ggtaaggggt
     6421 aaagggtaag gggtaaaggg gaaggggtaa gggaaacgca ttcgcgcctt cttcccttac
     6481 cccttcccct ttacccctta cccgtttcct tccctaccgg cgcaccttgt tgaagtgcag
     6541 ccgcagcggc tcggcgagtg agatgtccca cagctcgaag atggcgtggc gcgggttctc
     6601 gattttcacc aactcgaaga cgcccgcgct gcccgaatgt tgccgcgtgc ccgcgcggtc
     6661 gtagaggtag cggctcgcct cgtcggcggt cgggcttcgc tcgctcctct gctcggcgag
     6721 cgcctcgacg gcgtaggact tcaggagctt cggccagtag cgcgcgaaga gaccgggact
     6781 ggcgaagaca tcggcccaga ccacctcgcc gtttcgggcg acgacgacgc cgacgacacc
     6841 cggccccaac agacgcccac ggagcgcgcc cacgtagctc tccacgctgt cggcgacctt
     6901 cttgctggcg tagacctgct ggtaagtgtc cgtgccgctc ccggtgccga gcttcgcgtt
     6961 gttctcgccg acctctttcc acacctcgct ctgctgcttc ttgtcctgcg ccgccgcgcg
     7021 caacgtcggg tgcgccaccg cgccgaggct gtagaagttt tccgacttcc gcgcgcccga
     7081 cggtgcgatg accggcgcag gcgcggcacc gccgccgccg ccgccgctcg cctcggccga
     7141 cgtgcgctgg ctccaacgcc cgtgctcgac gcagaagacg ctcaggctga tggggacgct
     7201 gacgggcggg atgacgacat catcctgaac tatccggtcc tgcttgccgc cgacgatcac
     7261 ctcgcccgcg agcagcatga gcttcctgcc cgaccggttg gtgagcgaca actcgttgac
     7321 gctcgcgttg tcagaacgca tcgacgcctg ctgcacggcg gcgctgccgc gccccgacgg
     7381 gcggcgctga agagtcgccg cggtgtcgcc gccgccgcgc tcggtgatga tgaccgtgcc
     7441 ggcgcggatg ccctcgtcga gtgtgatgta ggcgtcggcg tcggccgtgg cgcctcttga
     7501 gtagacgggg aacagcgtga ggttcttgta gctgatggga cggccgacgg actgtgtctg
     7561 ccagcccgac ttcttcggcg gcccgtcgcc cgtcggcaac gccgccgcgg cccccgccaa
     7621 catcaacact gaaaggcaca gcgccagcca cccccgcgtc gaaacaaaac tcgatttcat
     7681 aatcttcctc ctcggatttc cgttgtcttt gttggaggtc ggccccgaac acatcgggac
     7741 ggcgggcgcg aggggcctgg ccgcctgcgg cagttcgatc cgtggcttct caccgatcaa
     7801 acacacgacc cttgttacac gggctgaacg cgcgttggcc gggactcttg cgtgacgtgg
     7861 aaaattttgt cgttgactgt gagcaacagt accgcgagcg gtagcgagcg ggtctgcctg
     7921 caatgacggc tcgcctcgcg cgtgtccgac ccgctcgcta ccgctcgcgg tactgttcat
     7981 accggctcgg ggcgctggca gggatgctcg gcgctcacgg gcgctcttgt cagaccggct
     8041 cggcggcgcg gccttcgggc aactcgtgtg gggcctcttg cacggcggcg cgctcggccg
     8101 gagtctcggg ctggcgacgg tagcggtggg tgacggggcg gcggcggccg acgccgatgg
     8161 cgttgcgcga gatgatgagg gtgggcggga gctgtcggcg cttgaactcg ttggccggcg
     8221 actcgacgcg gttgaggact tcgtaggtga gcgccgcgtc ggagccttcg gcgatgatct
     8281 gctcgggcgt cgccttgcgc tcgaagtaca tttgcaggat ggggtcgagc acgtcgtagg
     8341 ccggcaagct ttggtcgtcg aactggtcgg gcgcaagctc cgcggacggg cgcttgttga
     8401 tgatcgccca cggaatgatt tcgcgctcgc ggttgatgtg gcgcgcaagc cggtagacct
     8461 cggtcttgag cacgtcgccg atgacggcca gtccgccgtt ggtgtcgccg tagagcgtgc
     8521 agtagccgag agcgagttcg ctcttgttgc cggtcgagag cagcagacga ccttcggcgt
     8581 tcgagatggt catcaggatg ttgccgcgca ggcgcgactg taagttctcg gccgcgaggc
     8641 tttcgccgcc gcgcgtcggc ctcgtcagcc cgagttgttt cgttaacacc tcgaaggcgt
     8701 cgatgatggg gtgcttgatg acgggcatcc ccaaactctt gccgaaggcg acggagtctt
     8761 cgacgctccc acgcgaggag aagggcgagg gcatcatcac gccgagcacg ttctccgcgc
     8821 cgagggcctc gcaggcgagc gcggcgacga cggccgagtc gatgccgccg gagagtccga
     8881 gcagggcgcg gcgaaagcgg ttcttgtgcg cgtagtcgcg aaggccgagg acgagcgcgc
     8941 tgcgcacggt ctcgatgtcg tcgccgggca ggcagcggtc gtcggctgtg ccgcagtcga
     9001 gttcgatggt gccgatgaac tcctcgaagg gcggggcctg gaggatggtc tcaccacggc
     9061 aatcggtgac gagcgacgcg ccgtcgaaga tgatgccgtc gttgccgccg accaagttga
     9121 cgtagacgat gggcagcccc gacgccttcg agcggtgcga gaccatcttg cagcgctcgc
     9181 ccatcttcga cttgttgaag ggcgaggcgt tgggcgagac gatcaggctc gcgcccatct
     9241 cgatcagctc gtcggtgggg tcgtgcgcgt agaggcgttc gcgccagaag gtcttgtcgt
     9301 tccagaagtc ttcgcagacg gggacgccga ggcgctcgcc gcccgcctcg aagagctggc
     9361 gctccgtggc cgactcgaag tagcgcggat cgtcgaacac gtcgtacacg ggcagcagcg
     9421 tcttgtcggc gaagccgagg agctggccgt tcgagatgac ggccgcggag ttgaaaagtt
     9481 ttctccccga agtctggccc gtcgggcgga cggtgccgac gacggccgtg attccttcgg
     9541 cggcggggat gatgcgctct aacggcttga gggccgagcg ggcgatgtcg gcgtcgagaa
     9601 accagtcgag cgaggtgtaa ccctgcacgc agacttcggg gaagacgatg aggtctgact
     9661 tttccgactt cgcctgctcg atggcgcgca ggattttttc ggtgttgccc tcgtagtcgc
     9721 cgttcgtcgt gttaatctgc ccgatggtga cgcgcataaa ctggtttcga ctctgcgaga
     9781 atcgatacgt gacttatcgc cggcggaagg ttcaggctgc ctcgacatac tcggccatac
     9841 tggtcggttc tggcacgggc agtccgtctt cgcgcaggcc gcgcaggtgc aactcgacag
     9901 cctcgcgcat ccgctgcttg atctccgcga ccgacttgcc ggtggcaatg cacccgggca
     9961 gatcaggtaa gtatgcggaa tagttccgct cgcctttctc gatgatcatg gcataacgca
    10021 tgtgcccgcg cctcctcact ccttaatccc ggcttgcgtc agaatgcttt tcaaagtctt
    10081 cggatgtatg tcatctcgcg ggtggcccga aatcgtcacg cggcccggcc gggccgggtg
    10141 cttgtattgg cggtgacttc cctcggtgac gacgtgatac cagccgtcct tctcgatcaa
    10201 cttaatcacg tcgcgcacct tcatacggcg tccgtctgct tcccggctct tgttaaggat
    10261 aacggaaaaa ggggtggaat gaaatggtct gcgccgtggg ccacggccca tgtacggccg
    10321 tctgcgaccg aaacgcgtgc ggccctcaag aatgtgctga aacagctact cttgagggcc
    10381 gcacgcgttt cgtggtttgc gtcgggtgtc ctactctctc gtcagtcgga gcgtcatcgg
    10441 ctcggacttc ttcggcgagt agagcttcag ctcgcccgtg ccgccggccc cgctcaggca
    10501 ggctcgggcg gcgccgaagc gtcgcggcgg cgcagcaccc accagagatg cgccccgtac
    10561 cagatgaaga agctcaacac ctgcgcgacg atgactgaga ggtgcgggcg cgcgggcggg
    10621 ccgccggtca tcgcgatgaa cacgatgagg ccgccgatga ggagcgccgt caggaggccg
    10681 gcgaggcgca cggcgcgcac gccttcgacg aagcgcggca gcgtcaggat ttggaagacg
    10741 acgttgaaga cgacgccggt cgcgatgagc gcgtcggcca tgttgccgac gaacgacggc
    10801 agcgactcac ggttgcgcat gtagagcggc gcggcgacta acagcgcgaa ggcgtaggcg
    10861 gcgagcgcca aaacgactgt tctcaggatt ctcatcattc ctcctccggc ccacagggcc
    10921 gcgcgctccg cggccgccgt ctttgcgaga tcgacgagcg cctcgcacca caccagcgcg
    10981 agcgccccgg cgcgcccctg ccgcctcacg tcacgacagc ggtcgttgaa gac
//
LOCUS       DASVES010000315         6020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_402110, whole genome shotgun sequence.
ACCESSION   DASVES010000315 DASVES010000000
VERSION     DASVES010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402110"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            568..1401
                     /locus_tag="VF240_12785"
     CDS             568..1401
                     /locus_tag="VF240_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176129.1"
                     /translation="MSEAITLYLAAAAPFGVAFFLRRHVSPRVRPRALALAALAGLAW
                     PATTLLMLSRKSTGGGESRSSEEAETSEERTVERGVRAVASALSDVGELCGATSVEKD
                     ERLSYVLFAARRAVERFAGLALVAKGASVSAAAAPRELELCRAAGRTGEDLRLAGRCT
                     HRRNVLRLLLHRERARNEMLHALAEVSETARRLAHEAHAPRRPTPRELSDAILRAYDL
                     ALEVLTGLDDERAVAGVRRLIDAERAHLRGPAGESSDSRRSAALLEEDYVRHERQLQR
                     L"
     gene            1367..2023
                     /locus_tag="VF240_12790"
     CDS             1367..2023
                     /locus_tag="VF240_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176130.1"
                     /translation="MYDTSGNYSVCDERPFTDPYLMHRIGWLRAEITRAEQRARLYRR
                     PDEESEARRMLDPMPTPEAFAVLGMFLGLFPPAVIFFRLFHYGFTPRHGLVVFLLCLG
                     MNIVCFAVGRAMGRFVGRKIDGLGRPSWPLALAASAALGLLWGVVTGGLGGAVFFGFG
                     APVGAAAAVPVGTLAFALFAPLHRLLARDGMIEARHAWPLVFGVTGLIAALIASPHVF
                     "
     gene            2142..3920
                     /locus_tag="VF240_12795"
     CDS             2142..3920
                     /locus_tag="VF240_12795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176131.1"
                     /translation="MLLALLLLSLLLLVLLGFYVFVAAPRSRAHQTFAAFVACLALWT
                     VKDIVMWGFGEAAAGAAAGWASVSFVTALLLQYALVVFAWVFPEGGRVPRGRAAVMFA
                     PGAVLVPAALAGLLWEEVGVSGGELYIRLSLLGYAFGLYIYGLFAYGFYLLARKYARH
                     RGTLWGKQLGAILLALVVTGALKTVANVALPLAGDYRLLPFSSTFVLAGVVIYAYAIT
                     SFRLFSLQSALDQFRLFPIAYKVALSIAAVAVLSFVLLQIPVVRWSFGVQTSDAWRRY
                     VVFSVISALLPNLILVALVIRIISRPLRRLTEAAVEVAGGSYGAQVELQSNDEVGLLA
                     ESFNEMSRKMADDIERLRAVGEQLARTEKLAAAGTLAAGVAHEVNNPLASISSLIQIL
                     QSRTTNGTPDEEARETLRLVQTQIARISQVLRDMMDFARTRPPARAPLDVREVVESGL
                     RFASFDKSFKRLQLTTDWDERAPMISADADQLQQVFLNLLLNARDAMPDGGELRVATR
                     FEARAGELAVEISDTGHGIPPEHRARVFDPFFTTKTAGAGTGLGLAVCYGIVNAHGGR
                     IDIVPNGGRGTTVRVTLPARPDALHG"
     gene            3923..4405
                     /locus_tag="VF240_12800"
     CDS             3923..4405
                     /locus_tag="VF240_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7176132.1"
                     /translation="MPTLHLETFVAAPAELCFDLCLDVNFHERAGHGRAVCGVTSGRL
                     QLGDTVTWEAVHFFVRQRLTSKIVAYERPRMFTDEMQRGAFARWRHAHRFVERPGGTL
                     IIDDVDFASPLGVLGKLVDAVLLESYMTRFLVRHNRHFKREAEAMVKDEAGGSRGKHT
                     "
     gene            4402..5760
                     /locus_tag="VF240_12805"
     CDS             4402..5760
                     /locus_tag="VF240_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7176133.1"
                     /translation="MSTRPLILVAEDEDLMRAIITRLLEAEGYRVSAVASAEAALEAF
                     AAEDVAVTLTDIRMAGMDGLALLDRIKDVDPEALVVVMTAYSSVDSAVAALRKGAYDY
                     VTKPFVNEDLLQSVKNALRQRELFRENRALRRELDRRYSFSEIIGTSESLQRVFRLVE
                     KVAGTNTNILINGESGTGKELVARAIHHNSARADRPFVAINCGALPEMLLESELFGHT
                     KGAFTGAVAARPGLFRSAEGGTIFLDEIGEVSPTMQVRLLRAVQEHEVTPVGASAAVR
                     FDARIICATNRDLEKEVSEGRFREDLFYRLNVIEVHLPPLRERREDIPLLVRHFIKRT
                     AREQGQSEKAVEPEAMTAFINYNWPGNVRELQNAVERAFTLSADSIDPDSLPPRVRDA
                     AAGQLPLRDPDGLRPTLEEVERRHILDTLASVNQDKTRAANILGIDLSTLYRKLKRYG
                     AA"
     gene            5793..>6020
                     /locus_tag="VF240_12810"
     CDS             5793..>6020
                     /locus_tag="VF240_12810"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7176134.1"
                     /translation="MEKRRIESFEDLMVWQRGIELVKQVYLQTSAGAFSRDFGLRDQI
                     RRAAVSIPTNIAEGFERSSRKEYLLFLNIAKG"
BASE COUNT          948 a         2066 c         1935 g         1071 t
ORIGIN      
        1 cgtttcgtgg tggcagaggt gggatgccgg gtgtgacatt ttcgtttccc gtgtccccga
       61 aatgcgacag cgccgccgct gccgcccgcg caatagctac gaacacactc ccatcgcgcg
      121 tggctccaat acgtacagtt cgctccgccc ggttccgcac tcagtgcgga aattcatttt
      181 catgtaactc cgggcgtgcc gacgccatcg atgccgccgc cgcattgacg acgacaccat
      241 tgctctccgg ataagcgaag ttaacacgat gctgcgctgg cgaagactca cgtcagccga
      301 cacgcgacct ccgacatgat tgccgtatgt ggtcgcgacg gcgcgcttga atccaacgag
      361 caatgctttg cagaatgcca acgcttgcgc cggcccgccg ctctttgcca acccacgcgg
      421 cccgcgtcac aagcacactt caccaacccc gcgcgccatc ggcccgcaaa atctttgaaa
      481 tgaagaggcg ccgcgcgcgt gcgctaacgc gcgccgaagc agcgcacgga cggcacgacc
      541 gttgccctgt tgcgtgtcgt catgcgcttg agcgaagcca tcacacttta cctcgcggcg
      601 gcggccccct tcggcgtcgc tttcttcctt cgccggcacg tgtcgccgcg cgtgcgcccg
      661 cgtgctcttg cgctcgccgc gctggccgga ctcgcatggc ccgcgaccac actccttatg
      721 ctctcgcgaa aatccactgg cggcggagag tcgaggtcga gcgaggaggc cgagacctcc
      781 gaggaaagaa ctgtcgagcg cggcgtgcgc gccgtggcct ccgcgctctc cgacgtgggg
      841 gaactctgcg gagccacgtc tgttgagaag gacgagcgtt tgagttacgt gctcttcgcg
      901 gcgcggcgcg cggtcgaaag gttcgcgggg ctcgcgctgg tcgcaaaggg ggcgagcgtg
      961 tcggccgcgg ccgctccgcg cgagctggag ttgtgccgcg cggcgggtcg cacgggcgaa
     1021 gacctccggc tcgccgggcg ctgtacgcac cgacgtaacg tccttcgcct gctcctgcac
     1081 cgcgagcgag cacgcaacga gatgctccat gcgctcgcgg aagtgagcga gacggcgcgc
     1141 aggctcgccc acgaagcgca cgcgcctcgc cggcccactc cgcgcgaact ctccgacgcg
     1201 attctgcgcg catacgacct cgcactcgaa gttctcacgg ggcttgatga cgagcgcgcc
     1261 gtcgccggcg tccggcggct gatcgacgct gagcgcgcgc accttcgcgg cccggccggt
     1321 gagtcgtccg attcgcgccg gagtgcggcg ctgcttgagg aagattatgt acgacacgag
     1381 cggcaactac agcgtctgtg atgagcgccc cttcactgac ccgtacctga tgcacaggat
     1441 cggctggctg cgcgccgaga tcacgcgcgc cgagcagcgc gcccgtctgt accgccgccc
     1501 cgacgaagag agtgaagccc gtcgaatgct tgatcccatg ccgacccccg aggcgttcgc
     1561 ggtgctcggg atgttcctcg gcctcttccc gccggcggtc atctttttca gactgttcca
     1621 ctacggcttc acgccgcgac acggactcgt cgtcttcctg ctgtgtctgg gaatgaacat
     1681 cgtctgcttc gccgtcgggc gcgcgatggg caggttcgtc ggccgcaaga tcgacgggct
     1741 ggggcgaccg tcctggccgc tcgcgctggc ggcgtcggcc gcgttgggcc tgttgtgggg
     1801 agtggtgacg ggcggcctcg gcggcgcggt cttcttcggg ttcggagcgc ccgtgggggc
     1861 ggcggcggcc gtgcccgtcg gcacgctcgc cttcgcgctc ttcgcgccgc ttcaccggct
     1921 gctcgcgcgc gatggtatga ttgaggcgcg gcacgcctgg ccgctcgtct tcggcgttac
     1981 ggggctgatc gccgcgctca tcgccagccc gcacgtcttt taggtgagca gtgagcagtg
     2041 agcagtaagc agtaaaaagc aatggccgac tagactgaac accggccacg tttcactcgt
     2101 cttccactgc ttactgctca ctgctcactg ctcactgcct tatgctgctc gcgctcctgc
     2161 tgctgtcgct cctgctgctc gtgctgctcg gcttctacgt cttcgtggcc gcgccgcgca
     2221 gccgcgcgca ccagacgttc gcggccttcg tcgcgtgcct cgcgctgtgg acggtgaagg
     2281 acatcgtgat gtggggcttc ggcgaggcgg cggcgggggc agccgccggt tgggcgagtg
     2341 tgagcttcgt gacggcgctg ctgttgcagt atgcgctggt cgtcttcgcg tgggtcttcc
     2401 ccgaaggcgg gcgggtgcca cgcgggcgcg cggcggtgat gttcgcgccg ggggcggtgc
     2461 tcgtgccggc cgccttggcg ggtttgctct gggaggaggt cggcgtctcg ggcggcgagc
     2521 tttacatccg cctgtcgctg ctcggttacg ccttcggcct ctacatctac ggcctcttcg
     2581 cttacggctt ttatctactc gcacgcaagt acgcccggca ccgcgggacg ctctggggca
     2641 aacaactcgg cgcgatactt ctggcgctcg tggtgacggg cgcgctcaag accgtcgcca
     2701 acgtcgcgct gccgctcgcg ggcgactacc gcctgctgcc cttcagctcc accttcgtcc
     2761 tcgccggcgt ggtcatctat gcttacgcca tcacgagctt tcggctcttc tcgctccagt
     2821 ccgcgctcga tcagttccga ctcttcccca tcgcctacaa ggtcgcgctc tcgatagcgg
     2881 cggtggccgt gctcagtttc gtgctgctgc aaattccggt cgtccgctgg agcttcggtg
     2941 tgcagacttc ggacgcgtgg cgccgctacg tcgtcttcag cgtcatctcg gcgctgctgc
     3001 cgaacctgat tctcgtcgcc ctcgtcatcc gcatcatctc gcgcccgctg cggcgtctga
     3061 ccgaggcggc ggtcgaggtg gccggcggct cctacggcgc gcaggtcgag ctacagagca
     3121 acgacgaggt ggggctgctc gccgaatcgt tcaacgagat gagccgcaag atggccgacg
     3181 acatcgagcg actgcgcgcg gttggcgaac agctcgcgcg cacggagaaa ctcgccgccg
     3241 cggggacgct cgccgccggc gtcgcccacg aggtgaacaa cccgctcgct tccatctcat
     3301 cgctcatcca gattttgcaa tcgaggacga cgaacggcac gcctgacgag gaggcgcgcg
     3361 agaccttgcg actcgtccag acgcagatcg cccgcatctc gcaggtgctg cgcgacatga
     3421 tggacttcgc ccgcacgcgc cccccggcgc gcgccccgct cgacgtgcgc gaagtggtcg
     3481 agtcgggtct tcgcttcgcg agcttcgaca agtcgttcaa gcgtcttcaa ctcacgaccg
     3541 actgggacga gcgcgcgccg atgatttccg ccgacgccga ccagctccag caagtcttcc
     3601 tcaacctcct cctgaacgcg cgcgacgcga tgcccgacgg cggcgagctg cgcgtggcca
     3661 cgcgcttcga agcgcgcgcc ggcgagctgg ccgtcgaaat ctccgacacc ggccacggca
     3721 ttcccccgga acaccgcgcg cgcgtcttcg acccgttctt caccacgaaa accgccggcg
     3781 cgggaaccgg cctcggcctc gccgtctgct acggcatcgt caacgcacac ggcgggcgca
     3841 tcgacatcgt cccgaacggc ggacgaggca cgaccgtccg cgtcacgctg cccgcgcgcc
     3901 ccgacgcgct tcacggatag cgatgcctac gcttcacctt gagacgttcg tcgccgcgcc
     3961 cgccgagctt tgttttgacc tctgtctcga cgtaaatttt cacgagcggg cgggccacgg
     4021 ccgcgccgtg tgcggggtca cttcggggcg gctgcaactg ggcgacacgg tgacatggga
     4081 ggccgtacac ttcttcgtcc gccagcgtct cacgtcgaag atcgtggctt acgagcggcc
     4141 gcggatgttc accgacgaga tgcaacgcgg ggcgttcgcg cgctggaggc acgcgcaccg
     4201 cttcgtcgag cggccgggcg gcacgctcat catagacgac gtggacttcg cctcgccgct
     4261 tggtgttttg ggcaaactgg tcgatgcggt cttgctcgaa agttacatga cgcgtttcct
     4321 cgtccgccac aaccggcact tcaagcgtga ggccgaggca atggtgaagg acgaggcggg
     4381 cggttcacgg ggtaaacaca catgagcaca cggccgttga ttctggtcgc cgaagacgaa
     4441 gacctgatgc gcgccatcat cacgcgcctg ctcgaagcgg agggctaccg cgtctcggcg
     4501 gtggcgagcg cggaggcggc gctggaggct ttcgccgccg aagacgtggc cgtcacgctc
     4561 accgacattc gcatggccgg gatggacggc ctcgccttgc tcgaccgcat caaggacgtt
     4621 gaccccgagg cgctcgtcgt cgtgatgacg gcctactcgt cggtcgattc ggcggtcgcg
     4681 gccctgcgaa aaggagccta cgactacgtc accaagccct tcgtcaacga agacctcttg
     4741 cagtccgtta agaacgccct gcgccagcgc gagctgtttc gggagaaccg cgcgctacgg
     4801 cgcgagcttg atcggcgcta cagtttttcg gagattatcg gcacgtcgga gagcttgcag
     4861 agggtgttcc gcctcgtcga gaaggtggcg ggcacaaaca cgaacatcct catcaacggc
     4921 gagtcgggca cgggcaagga gctggtagcg cgcgccatcc accacaacag cgcgcgcgcc
     4981 gaccgcccct tcgtcgccat caactgcggc gcgctgcccg agatgcttct ggagtcggaa
     5041 ctcttcggcc acacgaaggg cgcgttcacg ggcgcggtcg ccgcgcgccc cggcctcttc
     5101 cgctcggccg agggcggcac cattttcctc gacgagatcg gcgaggtcag cccgacgatg
     5161 caggtgcgcc tgcttcgcgc cgtgcaggag cacgaggtga cgcccgtcgg cgcgagcgcc
     5221 gccgtccgct tcgacgcgcg catcatctgc gccaccaacc gcgatttgga gaaggaagtg
     5281 agcgaaggcc gcttccgcga agacttgttc taccgcctca acgtcattga agttcacctg
     5341 ccgcccctgc gtgagcggcg cgaggacatc ccgctcctcg tccgccactt catcaaacgc
     5401 acggcgcgcg aacaggggca gtccgagaag gccgtcgagc cggaggctat gaccgccttc
     5461 atcaactaca attggcccgg caacgtccgc gaactccaga acgccgtcga gcgcgctttc
     5521 actctcagcg ccgactccat cgaccccgac agcctcccgc cgcgcgtgcg cgacgcggcc
     5581 gccggccaac tccccctgcg tgaccccgac ggcctccgcc ccacgctcga agaggtcgag
     5641 cgccgccaca tcctcgacac cctcgcctcc gtcaatcagg acaagacccg cgccgccaac
     5701 atcctcggca tcgacctctc cacgctctat cgcaagctca aacgctacgg cgcggcataa
     5761 gtaaaggggt aagggggaaa ggggaagggg taatggagaa aagacggatt gagagttttg
     5821 aagacctaat ggtgtggcag cgtgggattg agctggtcaa gcaggtgtac ctgcaaacta
     5881 gcgccggcgc tttcagcaga gattttggtt tgcgcgatca gattcgacgt gccgcagtct
     5941 cgattccgac taacatcgcc gagggctttg agcggtcttc gcgcaaagaa tacttgttgt
     6001 tcctcaatat cgctaaaggg
//
LOCUS       DASVES010000316        15589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_32854, whole genome shotgun sequence.
ACCESSION   DASVES010000316 DASVES010000000
VERSION     DASVES010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15589
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32854"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..529
                     /locus_tag="VF240_12815"
     CDS             71..529
                     /locus_tag="VF240_12815"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEX7176135.1"
                     /translation="MNVIRLPVQKMTREAFAPFGMLIDSRGAREIDLGGGVPSLTGAT
                     AERRPFGFEFMARHTRTMQAFAPLASLEAIICVAPPNEEGAPEIEKVVAFRVEGRLPY
                     AYHKGTWHTPPFAVGEWYSYLVIDRAGTLDDDYELVDLKTARGCAFEIEI"
     gene            557..1012
                     /locus_tag="VF240_12820"
     CDS             557..1012
                     /locus_tag="VF240_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176136.1"
                     /translation="MSTERLTEILNYLSAMSRDIGAFRSETNARFDALEARVGKIEVR
                     LDSIEVRLDNIEVRLDNIEARLDKLEGRMDKLEGRFDSLEGYVGRLETSMVARFEAMN
                     VKFDEFRSDVRRLNRKFDLVSEDSTELRSDYHDMNKRVDALERKTGILE"
     gene            1015..2346
                     /gene="nuoH"
                     /locus_tag="VF240_12825"
     CDS             1015..2346
                     /gene="nuoH"
                     /locus_tag="VF240_12825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX7176137.1"
                     /translation="MDVFALFAPPTAIDGLLLKLFEPQTVNNVVWPFLQIGAVVTAIA
                     AWAAYATYLERKISAFMQARLGPMRVGKWGLLQPIADAIKLLTKEDIEPDKADHHIFR
                     LTPYIAVVAAFVVFAVVPFAPDWGVITDINIGLLFVLSVSSVGVLALILAGWASNSKY
                     ALLGGLRSSAQMVSYEVSMGLSLIGALMFARSLSLANIVHAQGGDTIWYIFYQPLGFV
                     IFLVSGIAENNRAPFDLPEAESELVAGFHTEYSGMRWSLFFMAEYAAMVVVSAIVTVV
                     YLGGWYFPGVYALTEARGYHTLYVVSGLVIFMVKMAVLLYVYFWLRWTFPRYRYDQLM
                     DLGWKWMIPASLINIFWTAVAIFTVQALDGWRGLDTVVSLRRGLELTRTGTAIMIAFG
                     VAGLFVAAGLLSAINSRSRDFNLKKQRRQVRLVSAPATRQAAPPAAVPDNV"
     gene            2376..2909
                     /locus_tag="VF240_12830"
     CDS             2376..2909
                     /locus_tag="VF240_12830"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX7176138.1"
                     /translation="MPEVTVPQPTWREKLKRFFLFDLIDGLKLTLKYNIGAFTDKDSV
                     AGRGIYTEQYPKERPIVAPRFHGAPRLNMDPETHESLCIACNLCALACPEDCIDVIPM
                     DIEMTVGGKARKKKVLDEFIFDTSRCMFCALCQEACPTACLELTQDFELATYSRAGFV
                     WNRKMLEQGREQPKYEA"
     gene            2995..3699
                     /locus_tag="VF240_12835"
     CDS             2995..3699
                     /locus_tag="VF240_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX7176139.1"
                     /translation="MILSGWEAIFFWVFAVAALVAGLLTIMARNAVHSALFLISTLVS
                     VAALFILLGAEFIAGVQILVYVGGVMVLFLFVIMLVNVGAEERNQENLFNHPTQVGAS
                     LVVLLLFVFGLIYAINQSAGRFRPVGAEAGRQGVAAEAQGRQRAARDRRLPLAETRTS
                     RLSRDTETVGASLYRYGGLPFEIASVLLLVAIVGSVLLARTTKQEAAVDDAEAQAAEA
                     DAPTRGEGERAGVENL"
     gene            3696..4094
                     /gene="nuoK"
                     /locus_tag="VF240_12840"
     CDS             3696..4094
                     /gene="nuoK"
                     /locus_tag="VF240_12840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX7176140.1"
                     /translation="MNSSIATLAALLLQETATQRIWQSLQYPDTSKFLVIAALLFVIG
                     VAGVLVRRNIIVIFMSVELILNAANLNFIAFARYFQETGSPNAVAGQVFTIFVIVVAA
                     AEAAIGLGIVIALYRNKETIFADKIDLLKW"
     gene            4174..6831
                     /gene="nuoL"
                     /locus_tag="VF240_12845"
     CDS             4174..6831
                     /gene="nuoL"
                     /locus_tag="VF240_12845"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7176141.1"
                     /translation="MHRFIWLIPLLPLAGAAINGLLGRRFRFSERVIGAVAVGSVALA
                     FLLGVAAVVSYGWGAHAVYPRPYVTSEEGGFPYSHTWIPGGAVEKTLGAAQAGAAERS
                     INPVQHSEGVRAETGAARVVVAPGGLLDVEWSYQLDPLSSIFILVVTGVGLCIFVFAT
                     GYMHGDPGFYRFFAYLGLFMFSMLILVMGSNFLMMFVGWEGVGLCSYLLIGYYFDRRE
                     AGDAARKAFITNRIGDFGFALAVFAVIAVFGSTQYTTVMEAARTYPIELLGTWGIMSW
                     IALGLFIGACGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGLYMVTRTNVIFQH
                     SQTMMLVVAVVGAATALFAATIGITQNDIKKVLAYSTVSQLGFMFLACGVGAFAIGIF
                     HVMTHAFFKALMFLGAGSVIHGMHHEQDMRRMGNLRKYMPKTYWTFLAGWLAICGIIP
                     FSGFWSKDEILWNTASTTYIPYGWLLWLVATIAATCTAFYMTRLVAMTFWGRERFLEV
                     PAGGQADEAHAHGYDEAAKPHDARTESAGDRPHHEPGEHVGVVHMHDTAQFDAGEHLD
                     AEGRDVHDDAGSHDPHAAGAHDAHGHGGHGSVVPHESPASMWVPLAVLAVLAVVGGFV
                     GISTAFTGGRHIGGRANIVNWLQPVIWNPATGRFGEAQGAEHGEVREQKSAASGLLAS
                     SDPSGATQKAESEAGHGDNALPYGDTGFNLAHSAEQALGSHLAAEWLFIIISLAVAAL
                     GIGLGILFYVKNPRLPEAWAARLGPLYRASFNKYWVDELYGALVTRRTMDAARGIYSI
                     DSKVVDGAVNGTASLTRRLSTLTGNADRIVVDGAVNGVAGFVKILMSPLIRAAQTGLT
                     PNYALVMVLGLVASVAFFFWRDIWAAMFG"
     gene            6976..8667
                     /locus_tag="VF240_12850"
     CDS             6976..8667
                     /locus_tag="VF240_12850"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7176142.1"
                     /translation="MKIPYLLSIITWLPAVGAVIVLALFKKGQADAIKKFTTAWFGLA
                     FLVSLGLLGYDRAAGGMQFLEDAQWIPTIGARYQMGVDGVSTLLIILTTLLGVIAALS
                     SWEYIGKREKEYYALLLLLQSAVVGVFASMDLFLFYLFFEVSLVPMYFLIGIWGGDNR
                     LYAAIKFFLYTLVGSVALLLGVIKLYFLTQDAALVGAITEATKLNVAGNTEALALLNS
                     TAAQARAAGTGTFNIMFLQSLGSVLPMGTMQVLLFFAFALGFAIKVPMWPFHTWLPDA
                     HVEAPTAGSVILAGILLKLGTYGFFRFNLPMFPRASADTAYWGSFGVRNVMVTLAIIG
                     IIYGALAAMYFVVKRDGDVKKLVAYSSVSSMGIVMLGLFALNPNGLNGAVLHMINHGI
                     YSGALFLLVGIIYERRHTRKVSEFGGLSHVMPGYAAVFLAMAMTAIGLPLLCVFISEF
                     LALRGAFEANPWWAGWGALGIVLNAGYMLWLYQRMFFGQIDNPKNEHLRDMKGREWAY
                     MLPLLVLSLWIGVYPKPFLEYIQRPVNSVVRQIRPNYPIPGAPAARATAERAVNK"
     gene            8727..9131
                     /locus_tag="VF240_12855"
     CDS             8727..9131
                     /locus_tag="VF240_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176143.1"
                     /translation="MFSRRRSVIASTLGVLLLTLSSIPAAAAVPQGRSTRIRIPRGRT
                     STAVRGVLTRRNSGGDYVLRARSGQKLFLDLIATREQTVLSLSSPTGENMAGEGGSGQ
                     MEFDLTETGDYKISVFNREGRRDTYRLAVRIR"
     gene            9214..10791
                     /locus_tag="VF240_12860"
     CDS             9214..10791
                     /locus_tag="VF240_12860"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7176144.1"
                     /translation="MFLLQASTTELFSLGDLRLIAPEIILTICACVALVMEVVLPYRK
                     ARWTGYFALTGLALAIVSVVMLGWPFLSGQAGTPLTGFYNTLRVDAFAVVFKLIFLAA
                     AALTVAVSLRYLDVEGEQRGEFYALVLFATVGMMFLASGYDLIVLYISLELMALTFYV
                     LVAYNRRSLRSNEAGMKYFLLGAFSSGVLLYGMSLLFGVAGSTNLGEIAPKVAQAVIV
                     NPDAAFAGLQLRPMVILAMIALAAGLFFKVSAVPFHMWAPDAYQGAPTPVTAFLSTGS
                     KAASFALFARLFIDGLGGMRADWAPLLGLVAALTILVGNWAAVTQANSKRLLAYSSVS
                     NAGYLLLGLVAANEFGYTGLVVYLIAYTFMNVGAFGVIISLRRRGILGDEVDDFNGLA
                     QKAPGMALMMTVFMLSLGGLPLTGGFIGKWFLFGGLLKRGADEGRNWYYWLAAWAALN
                     TVVSFYYYVRFIRAMYLGEREAEPRPLSLSPALSAALVVCVVGVIATGVYPWPFISLA
                     HNLLETLAAAGAVALQR"
     gene            11002..12153
                     /locus_tag="VF240_12865"
     CDS             11002..12153
                     /locus_tag="VF240_12865"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7176145.1"
                     /translation="MKQEKTMTPKTTLPHSQKFSALRLPALALSLVVLLAGPAAAVRA
                     DNPHDRPLGDSRVLAPFPANPGYPEGIAVDGRTVYVSGPAQFGNFLQPVVVAYDIDTG
                     AVVAQYPLAGQNPFAPQAGTGLAFDNHDVLYVGDLQQGVISIRVEEEGAPQDVYATAL
                     PDLPTCSTAPAPCSPTAIDRPPLINDLVFDHAGNLYITDSFQATIWRVAEGGGAPQIW
                     FQSPTFDTDFGPNGMRFDEKGERAYVSVTFNAFGQGFIYTLPAVAQPTQGDLALFHQY
                     NAGEGPDGIAFGKSGNLYVALAASNQISVLRPDGTEEVRYGGPSANPGGVLDPLPWAN
                     PAAIAFNDQTRSLLVTNHAVFFPNPGPFFAIFDVYVNDKASKLERPNIN"
     gene            12371..13144
                     /locus_tag="VF240_12870"
     CDS             12371..13144
                     /locus_tag="VF240_12870"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7176146.1"
                     /translation="MERELAGRAALVTGATSGIGRATALRFAEAGAGVALVGRNVEAL
                     AEVAAEARARGAEAIEVRADVKSEEDAARAVAESAAAFGRLDVLVSAAGILGTGTVET
                     TTLDAWDEMLNVNLRAVFHLMQLTIPHLEKTRGNVVNVSSVTGLRSFPGVLAYCVSKA
                     GLDQLTRCAALELAPKGVRVNAVNPGVVSTGIHLRGGMEADAYSAFLEHSKQTHPLGR
                     VGSPEEVAELILFLASERASWITGATHSIDGGRAQTCAR"
     gene            13268..14479
                     /locus_tag="VF240_12875"
     CDS             13268..14479
                     /locus_tag="VF240_12875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7176147.1"
                     /translation="MILFYVIAGLVLLQSAVSLRGGFRYLAFFRREAGAAPPVYTPFA
                     SVIVPCRGIDQGLTENFAALFAQDYPAYEIVFVGDRREDPALALAEELRGSLRGGGVT
                     RSRVVVAGAARESGQKVYNLLAAVREADAASEVFVFVDTDARPQAGWLRSLIAPLAHS
                     GTGAATGYRWFIPVRGGLASHLRSVWNASIASALGPDTRRNFCWGGSTAVRRETFERL
                     GVAEAWRGALSDDYALTRTLQSAGLPIHFVPTCLTPSFEDCTFGELFEFTTRQLKITR
                     VYAPHLWKIVLFSNLLFTLAFFGGGALVAARAALGLPFAAPLALLCAIFLTGALKAFV
                     RLRAVAISLAPHRARIWRGAWAHLLLWPLTAALFLYNALAAARSRRITWRGITYELIS
                     PTETAIIQSDK"
     gene            14564..>15589
                     /locus_tag="VF240_12880"
     CDS             14564..>15589
                     /locus_tag="VF240_12880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7176148.1"
                     /translation="MSNKAVQLYKLLRSTGRERRLGTVIFFITSRCNARCETCFYHEE
                     LNRPGDLTFAEIERVSRTMPPITDLWLSGGEPTLRHDASEVIDLFVRNNGVRRLIIPT
                     NGLIKQRAFEIVERALESHPELHLYLNVALDGYGETHDRIRGVPGNWQKTLDCIRSLY
                     PLKDRFSERFRLNVNTVVCAENYTEIDRLAGFMWENFRLDGHYFNIIRGDALAGDQIK
                     QVPPGALSQIYERAAALTDAYGRRMFAADDGASRFVKQVAYVGAITTHYRTQRANFEG
                     PAAWPFPCTAGETTAVIDYNGDVRACELRESFASLRDFDFDFGALWDSQPRAAELEGI
                     DGGRACWC"
BASE COUNT         2528 a         5319 c         4801 g         2941 t
ORIGIN      
        1 tgataagtga taagacaaaa accgtcttct ctttcttatc actcatcact tatcactcat
       61 cactgctttt atgaatgtta tccgcctccc ggttcagaag atgacgcgcg aggcgttcgc
      121 gccgttcggg atgctgattg atagccgcgg cgcgcgcgag atcgacctcg gcggcggcgt
      181 cccgagtctg acgggcgcga ccgccgagcg ccggcccttc ggcttcgagt tcatggcgcg
      241 ccacacgcgc acgatgcagg cgttcgcgcc tctcgcctcg ctggaggcga tcatctgtgt
      301 agcgccgccg aacgaggagg gcgctcccga aattgaaaag gtggtggcgt tccgcgtcga
      361 gggccgcctg ccctacgcct accacaaggg cacgtggcac acgccgccct tcgccgtggg
      421 cgagtggtac tcatatctcg tcatcgaccg cgccggcacg ctcgacgacg attacgagct
      481 ggttgacctg aagacggcgc gcggctgcgc gttcgagatt gaaatctgaa cctcacctca
      541 gagaaaggat gtaaagatga gtacagaacg cctcaccgaa atactcaact acctcagcgc
      601 gatgagccgc gacatcggcg cgttccgctc ggagaccaac gctcgtttcg atgccctcga
      661 agcccgcgtc ggcaaaatcg aagtccgcct ggacagcatt gaagtacgac tcgataacat
      721 cgaagtacgt ctcgataaca tcgaggctcg tctcgacaag ctcgaaggcc ggatggacaa
      781 gctcgaaggc cgcttcgaca gtcttgaggg gtacgtgggt cgcttagaga cctcgatggt
      841 cgccagattc gaagcgatga acgtgaagtt cgacgaattc cgctccgacg tgcgccgcct
      901 gaaccggaag ttcgacctcg tctcggaaga ctctacggaa ctgcggagcg actaccacga
      961 catgaataaa agggtggacg cgctcgaaag aaaaacgggc attctggaat aactatggac
     1021 gtgttcgcgc tgtttgcgcc accgaccgcc atcgacggcc tgctgctgaa actcttcgag
     1081 ccgcagacgg tcaacaacgt cgtctggccg ttcctccaga tcggggcggt ggtgacggcc
     1141 atcgccgcgt gggcggccta cgccacctat ctggagagga aaatctcggc cttcatgcag
     1201 gcgcgtctcg ggccgatgag ggtcgggaag tgggggcttt tgcagcccat cgccgacgcc
     1261 atcaagctgc tgacgaagga agacatcgag ccggacaagg ccgaccacca catcttccgc
     1321 ctgacgccct acatcgcggt cgtcgccgcc ttcgtcgtct tcgccgtcgt tcccttcgcg
     1381 cccgactggg gcgtcatcac cgacatcaac atcgggctgc tcttcgtcct ctcggtgtcg
     1441 tcggtgggcg tcctcgcgct gattctcgcg ggctgggcgt cgaactcgaa gtacgcgctc
     1501 ttgggcggcc tgcgctcgtc ggcgcagatg gtctcctacg aagtctcgat ggggctgtcg
     1561 ctcatcggcg cgctaatgtt cgcgcgctcg cttagcctcg ccaacatcgt ccacgcgcag
     1621 ggcggcgaca ccatctggta catcttctac cagccgctcg gcttcgtcat cttcctcgtc
     1681 agcggcatcg ccgagaacaa ccgcgcgccg ttcgatttgc cggaggcgga gtcggagctg
     1741 gtcgccggct tccacacgga atactcgggg atgcgctggt cgctcttctt catggcggag
     1801 tacgcggcga tggtcgtcgt ctccgcaatc gtcaccgtgg tctacctcgg cggctggtac
     1861 ttccccggcg tttacgctct gacggaagct cgcggctatc acacgctcta cgtcgtttcg
     1921 ggactcgtca tcttcatggt gaagatggcc gtgctgctct acgtctactt ctggctgcgc
     1981 tggacgttcc cgcgttaccg ctacgatcag ttgatggatt tgggctggaa gtggatgatt
     2041 ccggcgtcgc tgatcaacat cttctggacg gccgtcgcca tcttcaccgt gcaggcgctc
     2101 gacggctggc gcggcctcga cacggtcgtc tccctgcgcc gtggacttga gctgacgcgg
     2161 acgggcacgg ccatcatgat cgccttcggc gtcgccggtc tgttcgtcgc cgccggcttg
     2221 ctctcggcca tcaactcgcg ctcgcgcgac ttcaacttga agaagcagcg ccggcaggtg
     2281 cgtctcgtga gcgcgcccgc aaccagacag gccgcgccac ccgcggcggt gccagacaac
     2341 gtgtaggaac gtgagcggaa ccgggggaga gatttatgcc tgaagtgact gtgccgcagc
     2401 cgacgtggag ggagaagctg aaacgcttct ttctgttcga cctgatcgac gggctgaagc
     2461 tgacgttgaa gtacaacatc ggcgcgttca cggacaagga ctcggtggcg ggccggggca
     2521 tctacacgga gcagtacccg aaggagcgcc ccatcgtcgc gccgcgcttc cacggcgcgc
     2581 cgcgtctgaa catggacccg gagacgcacg aaagcttgtg catcgcctgc aacctctgcg
     2641 cgctcgcctg ccccgaggac tgcatcgacg tgatccccat ggacatcgag atgacagtcg
     2701 gcggcaaggc gcgcaagaag aaggttctcg acgagtttat cttcgacacc tcgcgctgta
     2761 tgttctgcgc gctctgccag gaagcctgcc cgacggcctg tctggagctg acgcaggact
     2821 tcgagctggc cacctactcg cgcgccggct tcgtctggaa ccgcaagatg ctcgaacagg
     2881 gcagggagca gcccaaatac gaggcgtagt gataagtgat gagtgataag tgataagaca
     2941 aaatcgtctt tcactttctt atcacttatc actcatcact tatcacttat cactatgatt
     3001 ctctctggtt gggaagcaat cttcttctgg gtgttcgcgg tggcggcgct ggtcgcgggc
     3061 ctgctcacga tcatggcgcg taacgcggtg cactcggcgt tgtttctgat ctcgacgctc
     3121 gtgtcggtgg cggcgctctt catcctgctg ggagcggagt tcatcgcggg cgtgcagatt
     3181 ctggtctacg tgggcggcgt gatggtgttg ttcctgttcg tcatcatgct cgtcaacgtg
     3241 ggcgcggagg agcgcaatca ggagaacctg ttcaaccacc ccacacaggt cggggcgagc
     3301 ctcgtggtgt tgctgctgtt cgtcttcggg ctgatctacg cgatcaacca gtcggcgggg
     3361 aggttccggc cggtgggggc cgaggcggga cggcaggggg tggcggccga agcgcagggg
     3421 cggcagcgcg cggcgcgcga ccgtcgcctg ccgttggccg agacgcgcac gtcgcgtctg
     3481 tcgcgcgaca cggagacggt cggcgcgagt ctctaccgct acggcgggct gcccttcgag
     3541 atcgccagcg tgctgctgct ggtggccatc gtcggctccg tgctgctggc gcgcacgacc
     3601 aaacaggaag cggccgtcga tgacgccgaa gcgcaagccg ccgaggcgga cgcgccgaca
     3661 cgcggcgaag gcgaacgggc gggcgtcgaa aacttatgaa cagctcaatc gcaactctcg
     3721 ccgcgctgct gttgcaggag accgccacgc agcgcatctg gcagagcctc cagtacccgg
     3781 acacgtcgaa gtttctcgtc atcgcggcgc tgctgttcgt catcggcgtg gcgggcgtgc
     3841 tcgtgcggcg caacatcatc gtcatcttca tgtcggtcga gctgattctg aacgccgcga
     3901 acctgaactt catcgcgttt gcacgctact ttcaggagac gggtagtccc aacgccgtcg
     3961 ccgggcaggt cttcacgatt ttcgtcatcg tcgtcgccgc ggccgaggcg gccatcggcc
     4021 tcggcatcgt catcgcgctc taccggaaca aagagacgat cttcgcggac aagattgatc
     4081 tgttgaagtg gtagttcaaa gttccgagtt caaagttcaa agtcaagacc tgactttccg
     4141 aactttgaac tttgaacttt gaaccttgaa cttatgcata gattcatctg gctcattcca
     4201 ctgctgccgc ttgcgggcgc cgccatcaac gggctgctcg gacgccggtt ccgattctcc
     4261 gagagggtga tcggggcggt ggcggtcggc tcggtggcgc tggcgttcct gctcggcgtc
     4321 gcggccgtgg tctcctatgg gtggggcgct cacgccgtct acccgcggcc ctacgtgacg
     4381 agcgaggagg gcggcttccc gtacagccac acgtggatac cgggcggggc ggtcgagaag
     4441 acgctggggg cggcgcaggc gggcgcggcg gagcggtcta ttaatcccgt ccagcacagc
     4501 gaaggcgtgc gcgcggagac gggcgcggcg cgcgtggtgg tggcgccagg cgggctgctc
     4561 gacgtggagt ggagctacca gctcgacccg ctctcgtcga tcttcatcct cgtcgtgacg
     4621 ggcgtgggcc tgtgcatctt cgtcttcgcc accggctaca tgcacggcga ccccggcttc
     4681 tatcgcttct tcgcctacct cggcctgttc atgttctcga tgctcatcct cgtgatgggg
     4741 tcgaacttcc tgatgatgtt cgtcggctgg gagggcgtcg gtctctgctc gtatcttttg
     4801 atcggttatt acttcgaccg cagggaggcg ggcgacgccg cgcgcaaggc gttcatcacg
     4861 aaccgcatcg gcgacttcgg cttcgcgctc gccgtcttcg ccgtcatcgc cgtcttcggc
     4921 tccacgcagt acacgaccgt catggaggcg gcgcgcacct acccgattga gctgctgggc
     4981 acgtggggca tcatgtcttg gattgcgctc ggcctcttca tcggcgcgtg cggcaagtcg
     5041 gcacagatac ctctgtacgt ctggcttccc gacgcgatgg ccgggccgac tccggtctcc
     5101 gccctcatcc acgcggcgac gatggtcacg gccggtctct acatggtgac gcgcacgaac
     5161 gtcatcttcc agcactcgca gacgatgatg ctcgtcgtcg ccgtcgtcgg cgcggcgacg
     5221 gccctgtttg ccgcgaccat cggcatcacg caaaacgaca tcaagaaggt gctggcttat
     5281 tcgaccgtct cgcaactcgg ctttatgttc ctcgcctgcg gcgtcggcgc gttcgccatc
     5341 ggcatcttcc acgtgatgac gcacgccttc ttcaaggcgc tgatgttcct cggcgccggc
     5401 tcggtcatcc acggcatgca ccacgaacag gacatgcgcc ggatggggaa cttgcggaag
     5461 tacatgccga agacttactg gacgttcctc gcgggctggc tcgccatctg cggcatcatc
     5521 ccgttctccg gcttctggtc gaaggacgag attctgtgga acaccgcctc gaccacatac
     5581 attccctacg gctggctgct ctggctcgtg gcgacgattg ccgccacctg cacggccttc
     5641 tacatgacgc ggctcgtggc gatgaccttc tggggccgcg aacgcttcct cgaagtcccc
     5701 gccggcggac aggccgacga ggcccacgcc cacggctacg acgaggccgc caagccgcac
     5761 gatgcccgca cggagtcggc cggcgaccgc ccgcaccacg agccgggcga gcacgtcggc
     5821 gtcgtccaca tgcacgacac ggcgcagttc gacgcaggtg aacacctcga cgccgaaggc
     5881 cgcgacgtac acgacgatgc cggatcgcac gacccgcatg ccgccggcgc acacgacgcg
     5941 cacggccatg gcgggcacgg ctccgtcgtc ccgcacgagt cgccggcctc gatgtgggtg
     6001 ccgctggcgg tgctcgccgt tctcgccgtc gtcggcgggt tcgtcggcat cagcaccgcc
     6061 ttcaccggcg gtcggcacat cggcggccgc gccaacatcg tcaactggct ccagcccgtc
     6121 atctggaacc ccgcgacggg acggttcggc gaagcacagg gcgcggagca cggggaggtc
     6181 agagaacaga agtcggcggc cagcgggctt ctcgcttcga gtgatccgtc gggcgcaacg
     6241 cagaaggccg agagcgaagc gggccacggc gacaacgcgc tgccctacgg cgacacgggt
     6301 ttcaacctcg cgcactcggc cgagcaggcg ctcggcagcc acctcgcggc cgaatggctc
     6361 ttcatcatca tctcgctcgc cgtcgccgcg ctgggcatcg gcctcgggat tctcttctac
     6421 gtgaagaatc cgcgcttgcc cgaggcgtgg gccgcgcgac tcggcccgct ctaccgcgcc
     6481 agcttcaaca agtactgggt cgatgagctg tacggcgcac tcgtcacgcg ccgcacgatg
     6541 gacgcggcgc gcggcatcta cagtatcgac tcgaaggtcg tggacggcgc ggtcaacggc
     6601 acggcgtcgc tgacccgacg gctgagcacg ctgaccggca acgccgaccg catcgtcgtg
     6661 gacggcgctg tcaacggcgt cgccggcttc gtcaagattc tgatgtcgcc gctcatccgc
     6721 gccgcgcaga cgggactgac gcccaactac gcgctcgtca tggtgctcgg cctcgtcgcc
     6781 tcggtggcgt tcttcttctg gcgggacatc tgggccgcta tgtttggtta gtcggaaaaa
     6841 ttaacatgga tggacaggat ggggaggata gaagattaaa aaggacagga tttgaactct
     6901 gactattgag atttgaaatc ttaaatccta tccatcctgt ccatccatgt taataatttt
     6961 ttcgggagtg tctcagtgaa aatcccttac cttctctcca tcatcacctg gctcccggcc
     7021 gtcggcgccg tcatcgtcct cgccctcttt aagaaggggc aggcggacgc catcaagaag
     7081 ttcacgaccg cgtggttcgg cctcgccttc ctcgtctccc tcggcctgct cggctacgac
     7141 cgggcggcgg gcgggatgca gtttttggaa gacgcgcagt ggattcccac catcggcgcg
     7201 cgctaccaga tgggcgtgga cggcgtctcg acgctgctca tcatcctcac cacgctgctc
     7261 ggcgtgatcg ccgcgctctc ctcgtgggag tacatcggga agcgcgaaaa ggaatactac
     7321 gcgctgctgc tgctgttgca gtcggccgtg gtcggcgtct tcgcgtcgat ggacttgttt
     7381 ctgttctacc tcttcttcga ggtgtcgctc gtgccgatgt acttcctcat cggcatctgg
     7441 ggcggcgaca atcgtctcta cgcggccatc aagttttttc tctacacgct cgtcgggtcg
     7501 gtggcgctcc tgctcggcgt catcaagctg tattttttga cgcaggacgc ggcgctcgtc
     7561 ggcgccatca cggaggcgac gaagctcaac gtcgcgggga acacggaggc gctggcgctc
     7621 ttaaacagca cggccgcgca ggcgcgcgcc gcgggcaccg gcactttcaa catcatgttc
     7681 ctgcaatcac tcggctcggt gctgccgatg gggacgatgc aggtgttgct gttcttcgcg
     7741 ttcgccttgg ggttcgccat caaggtgccg atgtggccgt tccacacgtg gctgccggac
     7801 gcgcacgtcg aagcgccgac ggccggctcc gtcatcctcg ccggcatcct cctcaagctc
     7861 ggcacctacg gcttcttccg cttcaacctg ccgatgttcc cccgcgcctc ggccgacacc
     7921 gcctactggg ggtctttcgg cgtccgcaac gtgatggtca cgctcgccat catcggcatc
     7981 atctacggcg cgctcgccgc catgtacttc gtcgtcaagc gcgacggcga cgtgaaaaaa
     8041 ctcgtcgcct attcgtccgt ctcctcgatg ggcatcgtca tgctggggct gttcgcctta
     8101 aacccgaacg gcctcaacgg cgccgtgctc cacatgatca accacggcat ctactccggc
     8161 gcgctcttcc tcctcgtcgg catcatctac gagcgccggc acacgcggaa ggtgtcggag
     8221 ttcggcggtc tgtcgcacgt catgcccggc tacgcggccg tctttctggc gatggcgatg
     8281 acggccatcg gcctgccgct gctgtgcgtc ttcatctcgg aattcctcgc cctgcgcggg
     8341 gcgttcgagg cgaacccgtg gtgggccggc tggggcgcgc tcggcatcgt cttgaacgcc
     8401 ggctatatgc tctggctcta ccagcggatg ttcttcggcc agatagacaa cccgaagaac
     8461 gaacacttgc gcgacatgaa ggggcgcgag tgggcttaca tgctgccgct gctcgtgctg
     8521 tcgctctgga tcggcgtcta tccgaagccg ttcctcgaat acatccagcg ccccgtcaac
     8581 tcagtggtgc gccagatacg gcctaactat ccgattcccg gcgcgcccgc ggcgcgggcc
     8641 acggcggagc gggccgtcaa caagtgacgc cggacactgc acgctcggcg ccgtccttaa
     8701 atactcacga acggaggaag accacgatgt tctcacgaag aagaagcgtc atcgcgagca
     8761 cgttgggcgt gctgctgctg actctcagtt ccatccccgc cgcggcggcc gtgccgcagg
     8821 ggaggagcac gcgcatccgc atcccgcgcg ggcgcaccag cacggccgtt cgcggcgtct
     8881 tgacgcgcag gaacagcggt ggcgactacg tgctgcgcgc ccgttcgggt cagaagctgt
     8941 ttctggacct gatagcgacc cgcgagcaga ccgtcctcag tctttcgtca cccaccggcg
     9001 agaacatggc gggcgagggc ggcagcgggc agatggagtt cgacctgacg gaaacgggcg
     9061 actacaaaat ctccgtcttc aaccgcgaag gccggcgcga cacctacagg ctcgcggtca
     9121 gaatccgctg acggcgcacg gctgaagccg ccgccgaggt tgcagaaaaa cagggggagc
     9181 gcggcgcgcc gccccgcgcc ccatccataa actatgttcc ttcttcaagc ctccacgacc
     9241 gagttgttca gcctcggcga cctgcgcctc atcgcgccgg aaatcattct caccatctgc
     9301 gcgtgcgtcg cgctggtgat ggaagtcgtg ctgccctacc ggaaggcccg ctggacgggc
     9361 tacttcgcgc tgacggggct ggcgctggcg attgtctccg tcgtcatgct cggctggccg
     9421 tttctctcgg ggcaggccgg cacgccgctc acgggtttct acaacaccct gcgcgttgac
     9481 gccttcgccg tcgtcttcaa gctcatcttc ctcgccgccg ccgcgctcac cgtcgccgtc
     9541 tcgctgcgct atcttgacgt ggagggcgag cagcggggcg agttctacgc gctcgtcctc
     9601 tttgccaccg tcgggatgat gttcctcgcc tcgggctacg acctcatcgt gctctacatc
     9661 agtctggagc tgatggcgct caccttctac gtcctcgtcg cctacaaccg gcgcagcctg
     9721 cgctcgaacg aggcagggat gaagtacttc ctgctcgggg ccttctcctc gggcgtgctg
     9781 ctctacggga tgtctctgct gttcggcgtc gccggctcga cgaacctcgg cgagatcgcg
     9841 ccgaaggtgg cgcaggccgt catcgtcaac cccgacgcgg cattcgccgg cctccagctc
     9901 cggccgatgg tcatcctcgc gatgatcgcg ctcgccgccg gtctcttctt caaagtctcg
     9961 gccgtgccct tccacatgtg ggcgcccgac gcctatcagg gcgcgcctac ccccgtcacg
    10021 gccttcctct cgacggggtc gaaggcggcg agcttcgcgc tcttcgcgcg cctcttcatc
    10081 gacggcctcg gcgggatgcg cgccgactgg gcgccgctct tgggtcttgt cgccgcactc
    10141 acgattctgg tcggcaactg ggcagccgtc acacaggcga actcgaagcg gttgctggcc
    10201 tactcgtccg tctcgaacgc cggctatctg ctgctcggcc tcgtcgccgc caacgagttc
    10261 ggctacacgg gcctcgtggt ctatctcatc gcctacactt ttatgaacgt cggcgcgttc
    10321 ggcgtgatca ttagcttgcg ccggcgcggc atcctcggcg acgaggttga cgacttcaac
    10381 ggcctcgcgc agaaagcgcc gggcatggcg ctgatgatga ccgtcttcat gctctcgctc
    10441 ggcggcctgc cgctgacggg cggcttcatc ggcaagtggt tcctcttcgg cggcctgctc
    10501 aagcgcggcg ccgacgaggg ccgaaactgg tactactggc tcgccgcgtg ggccgccctc
    10561 aacacggtcg tctccttcta ctactacgtg cgcttcatcc gcgccatgta cctcggcgag
    10621 cgcgaggccg agccgcgccc gttgtcgctg tcgcccgcgc tttccgcggc gctcgtcgtc
    10681 tgcgtcgtcg gcgtcatcgc gaccggcgtc tacccctggc ccttcatctc gcttgctcac
    10741 aacctgctcg aaaccctcgc cgccgccggc gccgtcgctc tgcaacgctg aacgcgggtc
    10801 gctttcctcc gccgcgcgcg gaagagtcta tccacccgcg tcaaaaatat tttcaccccg
    10861 cctgatccct ccgccctcct ttttccgctt taagtaggtg aaccccgaac ggggtctgag
    10921 cttttccaac tcggagagcg ggccgcgccc gcgatccctg aggtgacacg ccggcctccc
    10981 cggccttcaa ggcggaaacc catgaaacag gagaaaacga tgacgcctaa gaccaccctc
    11041 ccacactcac aaaagttctc ggcccttcgc ctcccggcgc tggcgctctc gctcgtcgtg
    11101 ctgttggccg gccccgccgc cgccgtcagg gccgacaacc cgcacgaccg gccgctcggc
    11161 gactcgcgtg tgctcgcgcc gttccccgcc aaccccggct accccgaggg catcgccgtc
    11221 gatggccgca ccgtctacgt ctccggcccg gcgcagttcg gcaacttcct ccagcccgtc
    11281 gtggtggcct acgacatcga caccggcgcg gtcgtcgcgc agtacccgct cgccggccag
    11341 aacccgtttg cgccgcaggc cggcaccggc ctcgccttcg acaaccacga cgtgctctac
    11401 gtcggcgacc tccagcaagg cgtcatcagc atccgcgtcg aggaggaggg cgcgccgcag
    11461 gatgtgtacg cgacggcgct gcccgacctc ccgacctgct cgaccgcccc ggccccctgc
    11521 tcaccgacgg ccattgaccg gccgccgctc atcaacgacc tcgtcttcga ccacgcgggc
    11581 aatctctaca tcaccgactc gttccaggcg accatctggc gagtagccga aggcggcggc
    11641 gcaccgcaaa tctggttcca gtcgccgacc ttcgacaccg acttcggccc caacggcatg
    11701 cgcttcgacg agaagggcga gcgcgcttac gtctccgtca ccttcaacgc cttcgggcag
    11761 ggcttcatct acacgctgcc ggccgtcgcc cagcccacgc agggcgacct cgcgctcttc
    11821 caccagtaca acgcgggcga ggggccggac ggcatcgcct tcggcaagtc gggcaacctc
    11881 tacgtcgcgc tcgccgcctc gaatcagatc agcgtgctcc ggcccgacgg gacggaagag
    11941 gtgagatacg gcggcccgtc ggccaacccc ggcggcgtgc tcgacccgct gccatgggcc
    12001 aacccggcgg ccatcgcctt caacgaccag acgcgctcgc tgctcgtcac caaccacgcc
    12061 gtcttcttcc cgaaccccgg ccccttcttc gccatcttcg acgtgtacgt caacgacaag
    12121 gcgtcgaaac tggagcggcc gaacatcaac tgacaggtaa aagtaaaaag gcaaaagtaa
    12181 aaaggcaaaa gtgaagaggg cgtgctgcgt tgtctgcctc ttcacttttg ccttttgcct
    12241 ttttactttt gccttcctcc gtggtgaacc gacttgtatt acactcaaga cgcagagcca
    12301 ttgaaacttc cgcgccctgc gacgacaata accgcgccgt aatcgatagc cgaggagagg
    12361 aacaactctg atggaacgtg agcttgcggg aagggccgct ctcgtgacgg gcgcgacctc
    12421 cggcatcggg cgcgcgaccg cgctccgctt cgccgaggcg ggggccggcg tcgcgctggt
    12481 cggcaggaac gtggaggcgc tggccgaggt cgccgccgag gcgcgcgctc gcggcgcgga
    12541 ggcgattgaa gtgcgcgcgg acgtgaagtc ggaggaggat gcggcgcgcg ccgtcgccga
    12601 gtccgcggcg gcgttcgggc gtctggacgt gctcgtcagc gccgccggca tcctcggcac
    12661 gggcaccgtc gagacgacga cgctcgatgc gtgggacgag atgctgaacg tcaatttgcg
    12721 cgccgtcttc catctcatgc agttgaccat cccgcatctg gagaagacgc gcggcaacgt
    12781 cgtcaacgtg tcgagcgtca cggggttgcg ctccttcccc ggcgtgctgg cctactgcgt
    12841 ctcgaaggcg ggcctcgacc agttgacccg ctgcgccgcg ctggagctgg cgccgaaggg
    12901 cgtgcgcgtc aacgccgtca accccggcgt cgtctccacc gggatacacc tgcgcggcgg
    12961 catggaggcg gacgcctaca gcgccttcct cgaacactcg aagcagacac acccgctcgg
    13021 acgcgtcggc agccccgaag aggtggccga actcatcctc tttctcgcct ccgagcgcgc
    13081 ctcctggatc accggcgcca cccactccat cgacggcgga cgcgcgcaga cctgcgcccg
    13141 ctaagacgca cgagggcgcg gacagtaccg cgagcggtag cgagcggttg cagccatgat
    13201 gagaggcccg ccacgcgcgc gttagacccg ctcgctaccg cttgctgcac tgtgcccatc
    13261 tctgttgatg attctcttct acgtcatcgc cggactcgtc ctactgcaaa gcgccgtctc
    13321 cctgcgcggc ggcttccgct acctcgcctt tttccgccgc gaggcaggag ccgcgccgcc
    13381 cgtttacaca ccgttcgctt ccgtcatcgt gccgtgccgc ggcatcgatc agggtctcac
    13441 tgaaaacttc gccgcgctct tcgcgcagga ctacccggca tatgaaatcg tcttcgtcgg
    13501 cgaccgccgc gaagacccgg cgctggcgtt ggcggaagag ttgcgcggga gtctacgggg
    13561 cggcggggtg acgcgttcgc gcgtcgtcgt cgccggcgcc gcgcgggagt cggggcagaa
    13621 ggtttacaac ctgctggccg cggtacgcga agctgatgcg gccagcgagg tcttcgtctt
    13681 cgtcgataca gacgcgcgtc cgcaggcagg ttggctacgc tcgctgattg cgccgctggc
    13741 ccactctggc acgggagcgg ccaccggcta ccgctggttc atccccgtcc gagggggact
    13801 cgcctcgcac ctgcgctccg tctggaacgc ctccatcgcc tcggccctcg gccctgacac
    13861 gcgccgaaac ttctgttggg gcggctcgac ggccgtcagg cgcgagacct tcgagcggct
    13921 cggcgtcgcc gaggcgtggc gcggggcgct ctcggacgac tacgcgctga cgcgcacact
    13981 tcaaagcgcc ggcctcccca tccacttcgt gccgacgtgc ctcacgcctt cgttcgaaga
    14041 ttgcaccttc ggcgaactct tcgagttcac gacgcggcag ttgaagatca cgcgcgtcta
    14101 cgccccgcac ctgtggaaga tagttctatt ttcaaacctg ctcttcacgc tcgccttctt
    14161 cggcggcggg gcactggtcg ccgcgcgcgc cgcgctcggt ctgcccttcg ccgcgccgct
    14221 cgccctcctg tgcgcgatct tcctgacggg ggcgctcaag gctttcgtcc gcctgcgggc
    14281 ggtcgccatt tcgctcgcgc cgcaccgcgc gcgaatctgg aggggcgcct gggcgcatct
    14341 gctcctgtgg ccgctgacgg ccgcgctctt cctctacaac gcgcttgccg ccgcgcgctc
    14401 gcgccgcatc acttggcgcg gcatcaccta cgaattgata tcgccgacgg aaactgccat
    14461 cattcagagt gataagtgat gagtgataag tgataagaag gaaacgactg ccgctaatct
    14521 ttttcttatc actcatcact tatcactcat caccttgctt ttcatgtcaa acaaagcggt
    14581 gcaactctac aagctcctgc gctcgaccgg ccgcgagcgg cggctcggca cggtcatctt
    14641 cttcatcacc tcgcgctgca acgcgcgctg tgagacctgc ttctaccacg aggagctgaa
    14701 ccgccccggc gacctcacct tcgcggagat cgaacgcgtc tcccgaacga tgccgccgat
    14761 caccgacctc tggctctcgg gcggggagcc gacgcttcgg cacgacgcct ccgaggtcat
    14821 cgacctcttc gtccgcaaca acggcgtccg ccgcctcatc atccccacca acggcctcat
    14881 taagcagcgc gccttcgaga tcgtcgagcg cgcgcttgag agtcatcccg agctgcacct
    14941 ctacctcaat gtcgcgctcg acggctacgg cgaaacgcac gaccgcatcc gcggcgtccc
    15001 cggcaactgg caaaagacgc tcgactgtat ccgctcgctc tatcccctca aagaccgctt
    15061 cagtgaacgc ttccgtctga acgtcaacac ggtcgtctgc gcggagaact acacagagat
    15121 cgaccgcctc gccggcttca tgtgggagaa cttccgcctc gacggacact acttcaacat
    15181 catccgcggc gacgcgcttg ccggcgacca gatcaagcag gtgccgcccg gtgcgctgtc
    15241 tcaaatctac gagcgcgccg ccgcgctcac cgatgcctac ggccgccgca tgttcgccgc
    15301 ggacgacggc gcctcccgct tcgtcaagca ggtcgcttac gtcggcgcca ttacgaccca
    15361 ctaccgcacg cagcgcgcga acttcgaagg ccccgccgcg tggcccttcc cctgcacggc
    15421 gggcgagacg acagccgtca tcgactacaa cggcgacgtg cgcgcctgcg agctgcgcga
    15481 gagtttcgcg agcctccgcg acttcgactt cgacttcggc gcgctgtggg actcgcaacc
    15541 gcgcgccgcc gagcttgagg gaatcgacgg cggccgcgcc tgctggtgt
//
LOCUS       DASVES010000317        18398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_402727, whole genome shotgun sequence.
ACCESSION   DASVES010000317 DASVES010000000
VERSION     DASVES010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18398
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402727"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /gene="radA"
                     /locus_tag="VF240_12885"
     CDS             <1..1224
                     /gene="radA"
                     /locus_tag="VF240_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX7176149.1"
                     /translation="GGVRLREVKPVAYGDIESQDDARVPSGIDEFDRVLGGGIVPGSL
                     VLIGGDPGIGKSTLLAQVADKISGQGLKVLYISGEESERQIKMRGERLGLKAENLYLL
                     PETNLERIMDEIERAEPAAVIVDSIQTVFSSRIESAPGSVSQVREVAGQFLMLAKNRE
                     IPVFLIGHVTKEGAIAGPKALEHIVDTVLYFEGERHYNHRIVRATKNRFGAANEVGVF
                     EMTGAGLVPVANPSEMFLKERPEGAAGSVVTACMEGTRPVLVEVQALVSGSKYGTGRR
                     MAQGFDANRMALLIAMLEKRVGFHLIGDDVFLNVAGGLEIDEPAADLGVVAAIASSFK
                     SLPIDAQTAVFGEVGLTGEVRGAMQAQARAREAQTLGFKKIVMPASNTAGLERLLGLR
                     VVGVRSVEEALDELF"
     gene            1383..2492
                     /locus_tag="VF240_12890"
     CDS             1383..2492
                     /locus_tag="VF240_12890"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX7176150.1"
                     /translation="MVFIAILVGAGYVLRPVPGNQFASSQLASALVALLVAAAIIFFE
                     MRIQRASLKTLIGAAIGSVLGIVGGVLIGFLITSQESGAVGANTKTFLTLALAFLMAY
                     IGLMVGAAKGDYLELSALGGIFSDKGARRDLKILDTSVIIDGRIADVSETGFLSGTIV
                     IPQFILRELQQVADSPDSSKRQRGRRGLDMLNRLQSNGKLDIQVVDTDFPAVKEVDLK
                     LLELSKSLDAVVVTNDFNLNKVAQLHGISVLNINELANSLKPVVLPGEGMRVFILKEG
                     KEYNQGVAYLDDGTMVVVDNARRMIGKNADISVTSVLQTTAGKMIFGRLWEDAHENGT
                     SEVRAIAGQRRGNTGRDLRPPTHAARPTTGETSGE"
     gene            2623..3330
                     /gene="ispD"
                     /locus_tag="VF240_12895"
     CDS             2623..3330
                     /gene="ispD"
                     /locus_tag="VF240_12895"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX7176151.1"
                     /translation="MNVAIIAAGGRGARMGGGARAPKQFLEIAGTPVVVHTLRRFEMC
                     ATIAEVFVVVPQGEVQAFLELAEKFSLSKLARVVAGGETRTESVWRGLQQLRAEDVGV
                     VAIHDAVRPLVTPEEIDATVRAAEATGAAVLAARAVDTIKESDGETVARTLERSRLWH
                     AQTPQCFRYDLLRRAYESARAFGFEATDDSSLVERLGISVAIVEGGARNIKITTPQDL
                     ALAEMLLKSGVRSQESE"
     gene            3403..3879
                     /gene="ispF"
                     /locus_tag="VF240_12900"
     CDS             3403..3879
                     /gene="ispF"
                     /locus_tag="VF240_12900"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX7176152.1"
                     /translation="MLRIGIGHDTHRLSEGRSLILGGVVIESERGGEGHSDADALTHS
                     LTDAVLGAICEGDIGVHFPDSDPRWKDADSLQLLARVVWLASQRGFHVVNADATVMLE
                     RPRLRPYMPEMRRKLAEVLGVDLECVSVKAKTGEGLDAVGAGAAVTALAVVLLERT"
     gene            3888..4184
                     /locus_tag="VF240_12905"
     CDS             3888..4184
                     /locus_tag="VF240_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176153.1"
                     /translation="MVDEQKLRDRITRALLSLEMKEKCANDVAFHMTDWVKDFEELAA
                     VWKNPAQFGDDQVTKAIYGFLAHVPNHLNAATKLVGMGPIEDVFGVGIFEEDKD"
     gene            4208..5530
                     /locus_tag="VF240_12910"
     CDS             4208..5530
                     /locus_tag="VF240_12910"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX7176154.1"
                     /translation="MRPQRVIRRKRDGGELTREEIAFFVRGVTTGAWADYQASALLMA
                     IFLNGMSDGEQQALTEEMLRSGEVLDFSSVPRPKADKHSTGGVGDKTSLILAPLAASC
                     GVAVPMISGRGLGHTGGTLDKLESIPGYRVDLHLAEFRTVLERVGFAMTGQTGEVAPA
                     DKILYALRDATETVESIPLIVASIMSKKLAAGLGALVLDVKTGSGAFMRDEEHSRELA
                     RALVATGNSCGVRTEALITDMSQPLGRAVGNALEVEECLDIMRGEQDEATRPVRELSL
                     ELAARMVALSGVAENLEAARERVTTALESGEALECFRRNVEAQGGDVSVCDDPARLRD
                     LTLDEVRVESPRAGFVGRVDAAEVGRAVASIGGGRVRMDDLVDPAVGYLAEARVGDRV
                     TEGQVLGLLYCRGAAAAEETAARILAAYEIMEGAPSAVPELFREVIDD"
     gene            5564..6607
                     /locus_tag="VF240_12915"
     CDS             5564..6607
                     /locus_tag="VF240_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7176155.1"
                     /translation="MKLKVFASVALLSAVALVASSCRGEAGAAEDRSKKRVGIVFDIG
                     GKDDRSFNAAAWEGVRRAAADLPIVLRDVEPGNPTSIEPAMRAFAERGYDLIIGIGFA
                     QAPIMEQVAKEYPHINFAIIDGVSQLPNVASLVFREHEGSYLVGMIAARKTKTNVLGF
                     VGGMDIPLIHRFKEGYEQGAKSVNPNIQVFANYVGINDSAWNNPGKGKELALSQINRG
                     ADVIFTAAGNSGLGAFDAAEQAGLDPQTGQATRYVIGVDSNQNGVKPGFVLTSMVKRV
                     DNAVYDAVKQVVDGNFKPGVQVFGLDKDGVAYSLDDFNRELIDPAVLAEVEAAKQQII
                     DKKITVVDAMGPK"
     gene            6747..7172
                     /locus_tag="VF240_12920"
     CDS             6747..7172
                     /locus_tag="VF240_12920"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7176156.1"
                     /translation="MKRKARARWQGGLQDGKGAISTDSGVLSDTQYSFSTRFENGVGT
                     NPEELIAAAHAGCFSMALSGQLGKADLKAESIETTATVTLDKVDGNFTITAVHLDVSA
                     RVPGASQEAFNTAAGNAKAGCPVSRVLNAEITMDARLES"
     gene            7264..8790
                     /locus_tag="VF240_12925"
     CDS             7264..8790
                     /locus_tag="VF240_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176157.1"
                     /translation="MLELRDITKRFGEVLANDGVSVRVEPGTIHAIVGENGAGKSTAM
                     RIAYGFYTADAGDILVDGQVRRISTPHDAIRLGIGMVHQHFMLVETMTVAENIVLGAE
                     PGSAAAIDLKQAGVRVRALSNEFRLAVDPEAVVENLSVGQQQRVELLKALYREARLLI
                     LDEPTAVLTPQEVEEFFQILRRMREQGKTVVIITHKLSEVLAISDDVTVMRDGRVVGS
                     VKTAETSAAELARMMVGREVLLRVEKAEGRPGEAVLSVRNLSVVADEGGRGVRDVTFD
                     VRAGEIVGIAGVEGNGQTELMEALAGLVAPERIHGAVELNGADITRMGARRRRESGIA
                     HVPEDRHRRGLLLDFDLAENSILGDHYRRPAVGGPGGVFLDNGGIRGRALEIINDFDV
                     RPANPDLPARALSGGNQQKLVIGREFELKPKLLLVSQPTRGVDIGAIEFIHRKLVEMR
                     ESGCAILLVSAELEEITSLADRLLVLYHGRVVGEVDPRTATQEEIGLLMTGGSKEGDE
                     "
     gene            8904..10019
                     /locus_tag="VF240_12930"
     CDS             8904..10019
                     /locus_tag="VF240_12930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7176158.1"
                     /translation="MRLFRELIFPLIAVAAAFVIGGLVVLLIGEDPIQTYRLLIGSAL
                     SWPDGIGYTLFYATPLIFTGLAVAVAFRCGLLNIGAEGQLYVAAFACAWVAIKLGGTQ
                     VDVFGTNVDYSWASLPAVVLVPLAVLAAVGAGALWGAIPGFLKARFGSHEVINTIMLN
                     FIAVALVSYFTQYHFKVQGDPILQTVPIGEAAHIPRLGQLVPGLPERIPLNVAFLLAI
                     LACALVYVFLWRTRWGYEIRATGANPTAAEYGGISTRKQIVLAMAVSGGLAGMVGINE
                     VLGYRYRFYDNFSDNYGYTGIAVALLGRNHPVGVIFSALLFGMLQRGSIHVDAFTENV
                     SKDLVQILQAIVILFVACEAFFRGTFGRFGLLRRSKI"
     gene            10110..11006
                     /locus_tag="VF240_12935"
     CDS             10110..11006
                     /locus_tag="VF240_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7176159.1"
                     /translation="MDNTIAATIVFFLASTIRQATPLLLAATGGLYSERAGVINIALE
                     GLMLAGAFTAAAVTHYTGNPYVGLLAGVLAGVGVALIHAVACIEFKADQVVSGTAINI
                     LMIGVPAVVSGAAFLSSGSTPQIPKENLIPWTPVVIAFLLVPVTWYVLYKTPFGLRLR
                     AVGEHPEAADAAGVRVKAMRYAGVLLSGALAGIGGAYLSTGQSSLFTRNMTSGRGFIA
                     LAALIFGKWRPVQTMLACILFGFTEALATQLQGVKISGDEEIPVQFIHLIPYVLTIVV
                     LAGFVGHSRAPRALGIPYQKER"
     gene            11165..12853
                     /locus_tag="VF240_12940"
     CDS             11165..12853
                     /locus_tag="VF240_12940"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX7176160.1"
                     /translation="MAISLKTEHLKRYKDIAWLLMKYGRSDLVKQAGLEEALTEETEK
                     FKDGVPAEARELAEDFERMGPTFIKLAQLLSTRSDLLPTPYLEALTRLQDKVEPFSFG
                     EVEEIVSKELGVRLSKAFAEFESQPVAAASLGQVHRATMRDGRHVVVKVQRPNIREQI
                     VDDLAALAEIAGFLDRHTEAGRRYGFVVMLEEFRKTLLRELDYRAEARNLTTIARNLS
                     EFERIIIPVPLEDYTTSRILTMDFVQGRKITDLGPLAQMDVDGHELADDLFRAYLKQI
                     LVDGFFHADPHPGNVFLTDDGSIALIDLGMVARLTPRFQESLLQILLAISEGRGDDVS
                     DIAIKMGEALPDFNEVNFRRQVGDLVAQHQGSSLDNMDAGRVGLEITRVAGENCFRLP
                     SDFTMMAKALLNLDQIVKTLDPVFDPNDAIRRHASEMMRERMMKSLSPGNLFMSAIEV
                     KDFVEKFPARVNRLLDAVANNDVRIKIDAIDETRLMEGFQKIANRITVGLVIAALIVG
                     AALIMDIQTSFTLFGYPGLAIIFFLIAAGAGLILIYNILFKDEKKKDDGPNAAP"
     gene            12891..13730
                     /locus_tag="VF240_12945"
     CDS             12891..13730
                     /locus_tag="VF240_12945"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX7176161.1"
                     /translation="MESLSPETLYERAEHAARVIRSRRAEEPRAALVLGSGLGAFADD
                     LEDSLAIPYEEIPGCARSTVEGHTGRLVLGRVGGVETAVMQGRFHFYEGYTLEEVTFP
                     IRVLGLLGVKSLVLTNAAGGLNNAYTEGALVCISDHLNLMGVNPLQGRNDERFGPRFP
                     DMTAVYDHEYQETAIREARACGLELRRGVYAALAGPSYETPAEIRMLRLLGADAVGMS
                     TVPEAIVARQMGLRVLGISCITNLAAGVLDRPIDHAEVMETGQRVREKFSELLRRVIP
                     KLM"
     gene            13827..14468
                     /gene="udk"
                     /locus_tag="VF240_12950"
     CDS             13827..14468
                     /gene="udk"
                     /locus_tag="VF240_12950"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX7176162.1"
                     /translation="MIIGICGGTGSGKTTVAKRILESVRACEVVFLQQDSYYRNLADL
                     ALVFRETVNFDHPDSIDNDLLVEHVRELKAGRSVELPVYDFKTHTRRAETLRVEPKPI
                     TIIEGILIFADPRLLAEMDIKVFVDTPDDIRFIRRLRRDVAERGRTVESVIEQYLTTV
                     RPMHMQFVEPSKRVSDVIIPEGGRNLVSVDLISGRIRERLGEERRRPEAAAGA"
     gene            14503..14913
                     /gene="cdd"
                     /locus_tag="VF240_12955"
     CDS             14503..14913
                     /gene="cdd"
                     /locus_tag="VF240_12955"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX7176163.1"
                     /translation="MEDQALIDAATSARDCALATYSNFCVGAAIESDDGRVFTGCNVE
                     SAAYELTMCAERVTIWKALSEGARRFTRIAVVADTERLTPPCGACRQLVWEFCGDIPV
                     ILANLRGDTETLQMSELLPRPFDAKFLEEASTQS"
     gene            14928..15389
                     /locus_tag="VF240_12960"
     CDS             14928..15389
                     /locus_tag="VF240_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176164.1"
                     /translation="MSDKEKADEAEPEKAEGGKAEGGAETVVERVINRYMGDTIHLFL
                     SLLSILIIAAAVLATYETLVRDFPKLLAPTDEYKVLQDIIGNILLIAIAAELGLLLLF
                     HRTSAAVEVIIFVIARKIVSPNLSSLDLLLAVAALAGLLIARFYYLPGKPK"
     gene            15431..16864
                     /locus_tag="VF240_12965"
     CDS             15431..16864
                     /locus_tag="VF240_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7176165.1"
                     /translation="MSKLSRRLFCVVLAGVVLWPLIPAEAQRRRASAATTVVDMLVVG
                     GTIVTMDERKRVIEDGAVAVARGRIVAVGARAEVERRYRLAPRGERIDAAGRVVIPGL
                     INGHTHAPMVLFRGLADDLELMDWLTKYIFPAEAKNVTEEFVRVGTRLALAEMIRGGT
                     TTYCDMYYFEDAIADETARAGVRGVLGETVIDFPVADNKTWAEAMAYSERFVKKWKGH
                     PLITAAVAPHAPYTVSEEHLKEVRALADRTGAPIVIHLAETRQEVADITKLKGVPPAE
                     YLARIGFLRPDVIAAHTVHLNEREIGLLKEHGVGSVHNPTSNMKLASGVAPVMSMLNR
                     EVRLGLGTDGAASNNDLDMWEEMDLAAKLHKLSANDTRVLPADLALALATLGSARALH
                     MEKEIGSLEEGKRADLVVVDIDGLHQTPLYNVYSHLVYATKASDVRTVVIEGRVVMRD
                     RRLLTLDEAVVKRQARVFRERISNSLK"
     gene            17022..18245
                     /locus_tag="VF240_12970"
     CDS             17022..18245
                     /locus_tag="VF240_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7176166.1"
                     /translation="MKGTRILRGARFAITAFSLALIFAPHTDLRAQQAPAEEAPEADV
                     VQRRVTRARALAAVGKLAAAAQDLEQLRTTAADDSVRDVASILLMNIYVEMPDYARAH
                     TLLEEAFKGRASRSEAAVRSHYALAGQTVGSVRAHVERYRSFGINVVDEDLPTEAKSD
                     LEQMRALLERVWEQAKSVREEEQKSGSGDRGADATALIEEAANVRLRLARGDEERARW
                     RQEVSEARQRLVAAETRITKVSDIVSAAAPAAGVSANNVYVPPASTTGPAPAQPAPKE
                     RAANASKKTAPAASNDNAPRPELRAESAKPADAAAPKQGGLFSVGSLQGLAAKKVSPS
                     YPSLAKSARITGVVTVFLVLNENGTVESVQRADGPAQLQTAATDAVRRWKFRQTLIDG
                     QPVKVSGYINFNFSL"
BASE COUNT         3345 a         6023 c         5909 g         3121 t
ORIGIN      
        1 ggcggcgtgc gtctgcgcga ggtgaagccg gtcgcttacg gcgacatcga gtcgcaggac
       61 gacgcgcgcg tcccctccgg catcgacgag ttcgaccgcg tgctcggcgg cggcatcgtg
      121 cccggcagcc tcgttctcat cggcggcgac cccggcatcg gcaagtccac gctgctcgcg
      181 caggtggccg ataagatcag cggtcagggc ttgaaggtgc tctacatctc gggcgaggaa
      241 tcggagcgac agatcaagat gcgcggcgag cgtctggggc tgaaggccga gaacctctac
      301 ctgctgcccg agactaatct ggaacgcatc atggacgaga tcgagcgggc ggagccggcg
      361 gccgtcatcg tcgattcgat tcagacggtc ttctcgtcgc ggatcgaatc cgcgcccggc
      421 tccgtctcgc aggtgaggga agtcgccggt cagtttctca tgctcgcgaa gaaccgggag
      481 atacccgtct tcctcatcgg ccacgtgact aaggagggag cgattgccgg gccgaaggcg
      541 ctcgagcaca tcgtggacac ggtgctctac ttcgagggcg aacgacacta caaccaccgc
      601 atcgtgcgcg ccacgaagaa ccgcttcggc gcggccaacg aggtcggcgt cttcgagatg
      661 acgggggcgg ggctggtgcc cgtggcgaac ccttcggaga tgttcctcaa ggagcgaccc
      721 gaaggggcgg ccggctcggt ggtgacggcc tgcatggagg gcacgcggcc cgtgctcgtc
      781 gaggtgcagg cgctcgtctc ggggtcgaag tacgggacgg ggaggcgcat ggcgcagggg
      841 ttcgacgcca accgcatggc gctcttaatc gcgatgctgg agaagcgtgt cggcttccac
      901 ttaatcggcg acgacgtgtt tctcaacgtt gccggcgggc tggagatcga cgagccggcg
      961 gccgacctcg gcgtggtcgc ggccatcgcg tcgagcttca agagtctgcc gatagacgcg
     1021 cagacggccg tcttcggcga ggtgggcctc acgggcgaag tgcgcggtgc gatgcaggcg
     1081 caggcgcgcg cccgcgaggc ccagacgctc ggcttcaaga agatcgtcat gccggcctcg
     1141 aacaccgccg gccttgagcg cctgctcggc ctgcgcgtgg tcggcgtgcg ctcggtcgaa
     1201 gaggcgctcg acgaactgtt ttagacgttg ccagggcggg cggtgagggg aacattgacg
     1261 gcagacggac tccgggccgc ccgaactgcg ctcagcacgc ccccgtaact tattcgtgac
     1321 gcgtcggttc ggcgcgtgtt aatatcctcg gcgtcatgcg gaccaatact ttcctcattc
     1381 ggttggtatt catcgcaatc ctggtcggcg cgggctacgt gctccgcccc gtgccgggca
     1441 atcaattcgc ctcgtcgcaa ctcgcctccg ccctcgtcgc cctgctcgtc gccgccgcca
     1501 tcatcttctt cgagatgcgt atccagaggg cgtcgctcaa gacgctcatc ggcgcggcca
     1561 tcggctcggt gctcggcatc gtcggcggcg tgctcatcgg cttcctgata acttcgcagg
     1621 agtccggcgc agtcggcgct aacacgaaga cgtttctcac tctggcgctc gccttcctga
     1681 tggcttacat cggcctgatg gtcggggccg cgaagggcga ctacttagaa ctctccgccc
     1741 tcggcggcat cttcagcgac aagggcgcgc gccgcgacct caagatactc gacacctccg
     1801 tcatcatcga cggccgcatc gccgacgtgt ccgagaccgg attcctttcc ggcaccatcg
     1861 tcatcccgca attcatcctc cgcgaactcc agcaggtggc cgactcgccc gactcctcca
     1921 agcgccagcg cggacgccgc ggcctcgaca tgctcaaccg cctccagtcc aacggcaagc
     1981 tcgacataca ggtcgtggac acggacttcc cggccgtcaa ggaagtcgat ttgaaactcc
     2041 ttgagttgtc gaagtcgctc gacgccgtcg tcgtcaccaa cgacttcaac ctcaacaagg
     2101 tggcgcagct ccacggcatc tctgtcctga acatcaacga gctggcgaac tccctgaagc
     2161 ccgtcgtgct gccgggcgaa ggcatgcgcg tcttcatcct caaggaaggc aaggagtaca
     2221 accagggcgt cgcctacctc gacgatggga cgatggtcgt ggtcgataac gcgcgccgca
     2281 tgatcggcaa gaacgccgac atctccgtca ccagcgtcct tcagaccacg gccggcaaga
     2341 tgatcttcgg acgcctctgg gaagacgcgc acgagaacgg cacgagcgaa gtccgcgcca
     2401 tcgccggcca gcgccgcggc aacaccgggc gcgacctgcg cccccccacc cacgcggcgc
     2461 gaccgactac cggcgagacg agcggagaat aaagacgtaa atcgtgaatc ataaatcgta
     2521 aatcgtgaat cgcggttgag gccggctcaa cttgaaggat gagccgcaag tcgaacgacg
     2581 gacaatcgat ttacgattta ccatttacga ttcacggctt ttatgaacgt tgccattatc
     2641 gccgccggcg ggcggggcgc gcggatgggc ggcggcgctc gcgcaccgaa gcagtttctg
     2701 gaaatcgcgg gcacgccggt cgtcgtccac acgctcaggc gcttcgagat gtgcgccaca
     2761 atcgccgaag tgttcgtcgt cgtcccccag ggggaagtac aagcttttct cgaactcgca
     2821 gaaaaattca gtctgagcaa actggcgcgc gtcgtcgcgg gcggcgagac gcgcaccgag
     2881 tctgtctggc gcggcctcca gcagttgcgg gctgaggacg tgggcgtcgt ggccatacac
     2941 gacgcagtgc gccccctcgt cacgcccgag gagatcgacg cgaccgttcg cgcggccgag
     3001 gcgacggggg ccgccgtcct cgccgcgcgc gccgtggata ccatcaagga gtcggacggc
     3061 gagacggtcg cgcgcacgct cgaacgctcg cgcctctggc acgcgcagac gccgcagtgc
     3121 ttccgctacg acctgctgcg gcgcgcctac gagagcgcca gagccttcgg cttcgaggcg
     3181 acggacgaca gctcgctggt cgagcgactc ggcatctcgg tcgccatcgt cgaaggcggc
     3241 gcgcgcaaca tcaagatcac caccccgcag gatttggcgc tggcagaaat gcttttgaag
     3301 tcaggagtca gaagtcagga gtcagaataa aagcaagagc gcccacttca ttcagcgcca
     3361 tcttcattct gactcctgac tcctgactcc cttgacttga gtatgctccg catcggcatc
     3421 ggacacgaca cgcaccgtct gagcgagggc cgctccctca tcctcggcgg cgtcgttatc
     3481 gagtccgagc gcggcggcga gggacactcg gacgcggacg ccctgacgca ctcgctcact
     3541 gacgccgtgc tcggcgccat ctgcgagggc gacatcggcg tccacttccc cgacagcgac
     3601 ccgcgctgga aggacgccga cagcctccaa ctgctggccc gcgtcgtgtg gctcgccagc
     3661 cagcgtggct tccacgtcgt caacgccgac gccacggtga tgcttgaacg cccgcgcctg
     3721 cgtccctaca tgccggagat gcggcgcaag ctggccgagg tgctcggcgt cgatttggaa
     3781 tgcgtcagcg tcaaggccaa gacgggcgag ggcctcgacg ccgtcggggc cggcgcggcc
     3841 gtcacggcgc tggccgtcgt ccttctcgaa cggacttagg aagtcggatg gtagacgaac
     3901 aaaagctgag ggacagaatc acacgtgcgc tgctttctct tgagatgaaa gagaagtgtg
     3961 cgaatgatgt tgccttccac atgacggatt gggtcaagga tttcgaggag cttgccgccg
     4021 tctggaagaa ccccgcacag tttggcgacg atcaggtcac taaggcaatt tacggcttcc
     4081 tcgcgcatgt gcccaatcat ttgaacgcgg cgacgaagct tgttggaatg ggtccgattg
     4141 aagacgtttt cggcgtgggc attttcgagg aagataagga ctgaagagcg gacgctgcca
     4201 tccttctatg cgaccacaga gggttattcg tcgaaagcgg gacgggggcg aactgacgcg
     4261 cgaggagatc gccttcttcg tgcgcggggt gacgacgggc gcgtgggctg actatcaggc
     4321 gtcggcgctg ctgatggcga tctttctcaa tggcatgagc gatggggagc aacaggcgct
     4381 caccgaggag atgctgcgct cgggcgaggt gctggacttc agttccgtgc cgcgcccgaa
     4441 ggctgacaag cattcaacgg gcggcgtcgg cgacaagacc tcgctcatcc tcgcgccgct
     4501 cgccgcctcg tgcggggtgg cggtgccgat gatctcgggg cgcggcctcg gccacacggg
     4561 cggcaccctc gacaagctcg aatccattcc gggctaccgc gtcgatctcc acctcgcgga
     4621 gtttcgcacg gtgctggagc gcgtcggctt tgcgatgacg gggcagaccg gcgaggtcgc
     4681 cccggccgac aagattctct acgcgctacg cgacgcgacc gagaccgtcg agtcgatccc
     4741 gctcatcgtc gcctcgatca tgtcgaagaa actcgcggcg ggcttgggcg cactcgttct
     4801 cgacgtgaag accggcagcg gcgctttcat gcgcgacgag gaacactctc gcgagcttgc
     4861 ccgcgcgctc gtcgccaccg gcaactcgtg cggcgtccga accgaggcgc tgattacgga
     4921 catgagtcaa ccactggggc gcgccgtcgg caacgccctc gaagtcgaag aatgtctcga
     4981 catcatgcgc ggtgagcagg acgaggcgac gcggcccgtg cgcgaactct cgctggagct
     5041 ggccgcgcgc atggtcgcgc tctctggcgt cgcggagaat ctggaggcgg cacgcgagcg
     5101 tgtgacgacg gcgctggagt cgggcgaggc gttggagtgc ttccgccgca acgtcgaggc
     5161 gcagggcgga gacgtgagtg tgtgcgacga ccccgcgcgt cttcgggatt tgacgctcga
     5221 tgaagtcagg gtagaatcgc cgcgcgcggg attcgtcggc agggtggacg cggccgaggt
     5281 cggacgcgcg gtcgcctcaa tcggcggcgg acgcgtccgc atggacgacc ttgttgaccc
     5341 ggcggtgggc tatctcgcgg aggcccgcgt cggcgaccgt gtgacggagg ggcaggtgct
     5401 cggactcctc tactgccggg gcgcggccgc cgccgaagaa acggccgcgc gcatcctcgc
     5461 ggcttacgaa atcatggaag gggcgccgtc cgccgtcccg gaactcttcc gggaagttat
     5521 cgacgactga agtccagagc ttgtagaaag aagcaatacc ggaatgaagc tcaaagtttt
     5581 cgcaagcgtc gcgctgctct cggccgtcgc gctcgtcgcc tcatcctgtc gcggggaggc
     5641 cggagccgcg gaggacaggt cgaagaagcg tgtcggcatc gtcttcgaca tcggtggtaa
     5701 ggacgaccgc tcgttcaacg ccgccgcgtg ggagggcgtc agacgcgccg ccgccgatct
     5761 gcccatcgtg ctgcgcgacg tggagccggg caacccgacc tccatcgagc cggcgatgcg
     5821 cgcgttcgcc gagcgcggct acgacctcat catcggcatc ggcttcgcgc aggcgcccat
     5881 catggagcag gtggcgaagg agtacccgca catcaacttc gccatcattg acggcgtgag
     5941 ccaactgccc aacgtggcct cgctcgtctt ccgcgagcac gaaggctcat acctcgtcgg
     6001 gatgatcgcc gcgcgcaaaa ccaaaacgaa cgtgctcggc ttcgtcggcg gcatggacat
     6061 cccgctcatc caccgcttca aagagggcta cgagcagggc gcgaagtccg tgaatccgaa
     6121 cattcaggtg ttcgccaact acgtcggcat caacgactcg gcgtggaaca accccggcaa
     6181 gggcaaggag ctggcgctgt cgcagatcaa ccgcggggcc gacgtaatct tcaccgccgc
     6241 cggcaactcc ggcctcggcg cgttcgacgc ggccgagcag gcgggcctcg acccgcagac
     6301 cgggcaggcc acacgctacg tcatcggcgt tgactcgaac cagaacggcg tcaaaccggg
     6361 cttcgtctta acgagcatgg tcaagcgcgt cgataacgcc gtctacgacg ccgtcaagca
     6421 agtagttgac ggcaacttca agcccggcgt tcaggtcttc ggtctcgaca aggacggcgt
     6481 tgcctactcg ctcgacgact tcaaccgcga actcatcgac ccggccgtgc tcgccgaggt
     6541 tgaagccgcc aagcagcaga tcatcgacaa gaagatcacc gtcgtggacg cgatggggcc
     6601 gaagtaacga agctattagc tattagctgt tagcttttag ccggaagaga gcgaaccggc
     6661 gcgttggctg tcagcggcgg cgcgcgtcac ggcgcatgcc gtgagctaac agctaatagc
     6721 taacagctaa tagccaagga gaacatatga aacgtaaagc acgggcgcgt tggcagggtg
     6781 gcttgcagga tggcaagggg gcgatttcta ccgacagcgg cgtcctctcc gatacgcaat
     6841 actcgttcag cacccgtttt gaaaacggcg tgggcaccaa cccggaggag ttgatcgcgg
     6901 cggcgcacgc cggctgcttc tcgatggcgc tctcgggtca gttgggcaag gccgacctca
     6961 aggccgagag catcgagacg accgccaccg tcacgttgga caaggttgac ggaaacttca
     7021 ccatcacggc cgtccacctc gacgtgtcgg cgcgcgtccc cggcgcgagc caggaggctt
     7081 tcaacacggc cgccggcaac gccaaggccg gctgccccgt ctcgcgcgtg ctcaacgccg
     7141 agatcacgat ggacgcgcgt ctggaatcgt aagaacgaag aggtcagagg tcagaggtta
     7201 gaggtcagtc aaagacctgc tttttaacta acctctaacc tctaacctct gacctctgct
     7261 tttatgctcg aactccgaga catcaccaaa cgattcggcg aggtgctggc gaacgacggc
     7321 gtgagtgtgc gcgtcgagcc ggggacgatt cacgccatcg tcggcgagaa cggggcgggg
     7381 aagtccaccg ccatgcgtat cgcctacggc ttctacacgg ccgacgcggg cgacatcctc
     7441 gtcgatggac aggtgcggcg catcagcacg ccgcacgacg ccatccggct cggcatcggc
     7501 atggtgcatc agcacttcat gctggtcgag acgatgacgg tggccgagaa catcgtgctc
     7561 ggggccgagc cgggcagcgc cgccgcgatt gatctcaagc aggcgggcgt gcgcgtccgc
     7621 gcgctctcga acgagttccg gctggcggtc gatcccgagg cggtcgtcga aaacctctcg
     7681 gtcgggcagc agcagcgcgt cgagttgctc aaggcactct accgcgaggc gcgtctgctc
     7741 atcctcgacg agccgacggc cgtcttgacc ccgcaggagg tcgaagagtt tttccagatt
     7801 ctccggcgga tgcgcgagca gggcaaaacg gtcgtcatca tcacgcataa gctctcggag
     7861 gttctggcca tctctgacga tgtgacggtg atgagagacg gccgcgtcgt cgggagcgtg
     7921 aagacggcgg agacgagcgc ggcggagctg gcgcgcatga tggtggggcg cgaggtgctg
     7981 ctgcgggtcg agaaggccga aggccgaccc ggcgaggcgg ttctgagcgt gcgcaacctc
     8041 tcggtcgtgg ccgacgaggg cgggcgcggc gtgcgtgacg tcacgtttga cgtgcgcgcg
     8101 ggtgagatag tcggcatcgc cggggtcgag ggtaacgggc agaccgagtt gatggaggcg
     8161 ctggccggac tcgtcgcgcc cgagcgcatc cacggggcgg tggaattgaa cggggcggac
     8221 atcacgcgca tgggcgcgcg gcgccgtcgc gagtcgggca tcgcgcacgt ccccgaagac
     8281 cggcatcggc gcggcctgct gctcgacttc gacctcgcgg agaactccat cctcggcgac
     8341 cactaccggc gcccggccgt cggcggcccc ggcggcgtgt tcctcgacaa cgggggcatc
     8401 cgcggacgcg ccctcgaaat catcaacgac ttcgacgtgc gcccggccaa ccccgacctc
     8461 ccggcccgcg cgctttcggg tggcaaccag cagaagctcg tcatcggccg cgagttcgag
     8521 ttgaagccga agctgcttct ggtctcgcag ccgacgcgcg gcgtggacat cggagccatc
     8581 gagttcatcc atcgcaagct cgtcgagatg cgcgagagtg gctgcgccat cctcctcgtc
     8641 tccgccgaac tcgaagagat cacctcgctc gccgaccgcc tcctcgtcct ctatcacggc
     8701 cgcgtcgtcg gcgaagtcga tccccgcacg gcgacacagg aggagatcgg tttgctaatg
     8761 acgggaggga gtaaagaggg tgatgagtga tgagtgataa gtgataagaa gctgttagct
     8821 cttggcgatt agctaacagc taagagctaa cagcttccgt ttttcttatc actcatcact
     8881 catcacttat caccattaaa accatgcgtc tgtttcgcga actcatcttt ccgctcatcg
     8941 ccgtggcggc ggccttcgtc atcggcggcc tcgtcgtgct gctcatcggc gaagacccca
     9001 tccagacgta ccgcctgctg atcgggagcg cgctgtcgtg gccggacggc atcggctaca
     9061 cgctcttcta cgccacgccg ctcatcttca cggggctggc cgtggccgtg gccttccgct
     9121 gcgggctgct caacatcggg gccgagggtc agctctacgt ggcggcgttc gcgtgcgcgt
     9181 gggtggcgat caagctgggc ggcacgcagg tggacgtgtt cggcacgaac gtggactact
     9241 cctgggcgag tctgccggcg gtcgtcctcg tgccgctggc ggtgctcgcc gccgtcggcg
     9301 cgggagcgct gtggggcgcg ataccggggt tcctcaaggc gcgcttcggg tcgcacgaag
     9361 tcatcaacac gatcatgttg aacttcatcg ccgtcgctct ggtcagctac ttcacgcagt
     9421 accactttaa ggttcagggc gaccccatcc tccagaccgt gcccatcggc gaggcggcgc
     9481 acatcccgcg cctcggccag ttagtgccgg ggctgcccga gcgcatcccc ttaaacgtcg
     9541 ccttcctgct ggccatcctc gcgtgcgcgc tcgtctacgt cttcctgtgg cgcacgcgct
     9601 ggggctacga gattcgcgcg acgggcgcca acccgacggc ggccgagtac ggcggcatct
     9661 cgacgcgcaa gcagatcgtt ttggcgatgg ccgtctccgg cggactcgcc ggcatggtcg
     9721 gcattaacga agtgctcggc taccgctatc ggttttacga caacttctcc gacaactacg
     9781 gctatacggg aatcgccgtc gcgctcctcg ggcgcaacca ccccgtcggc gtcattttct
     9841 cggcgctgct gttcggcatg ctccagcgcg gctcaatcca cgtggatgcc ttcaccgaaa
     9901 acgtctcgaa agacctggtg caaattctcc aggccatcgt catcctgttc gtcgcctgcg
     9961 aggcattctt ccgcggcacc ttcggccgct tcgggttgct gaggaggtcg aagatttgaa
    10021 aagaagaagt caggagccag gagccaggag tcagaataaa aacgacttcc tgcttcttct
    10081 gactcctgac tcctgactcc tgacttccta tggataacac catcgccgcc accatcgtct
    10141 tcttcctcgc ctcgaccatc cggcaggcga cgccgctgct gctggcggcg acgggcggtc
    10201 tctactcgga gcgcgcgggc gtcatcaaca tcgcgctcga aggcttgatg ctggccggcg
    10261 cgttcacggc cgccgccgtg acgcactaca cgggcaatcc ttacgtgggt ttgctggccg
    10321 gcgtgctggc cggggtcggg gtggcgttga ttcacgccgt cgcgtgcatc gagttcaagg
    10381 ccgatcaggt ggtgagtggg acggccatca acattttgat gatcggcgtg ccggcggtgg
    10441 tgagcggcgc ggcctttctc tcgtcgggtt cgacgccgca gattccgaag gagaatctga
    10501 taccgtggac gcccgtcgtg atcgcgttcc tgctggtgcc ggtgacgtgg tacgtgctct
    10561 ataaaacgcc gttcggcctg cgcctgcgcg ccgtgggcga gcacccggag gcggccgacg
    10621 cggcgggcgt gcgcgtgaag gcgatgcgct acgcgggcgt gctgctctcg ggcgcgctcg
    10681 ccggcatcgg cggcgcgtat ctttcgacgg gtcagtcgtc gctctttacg cgcaacatga
    10741 cctcggggcg cggcttcatc gcgctcgccg cgctcatctt cggcaagtgg cgacccgtgc
    10801 agacgatgct cgcctgcatc ctcttcggat tcaccgaggc gctggccacg cagcttcagg
    10861 gcgtcaaaat ctccggcgac gaagagatac ccgtgcagtt catccacctc atcccctacg
    10921 tcctaaccat cgtcgtgctc gccggcttcg tcggccactc gcgcgcgccg cgtgcgttgg
    10981 gcatcccgta ccagaaggag aggtgaagtc aggagtcagg agtcaggagt cagaataaaa
    11041 cgcctcgtgc ggcattactg ccctgcggca gacctgacaa cttcgcggtt gagagataca
    11101 atctcgtcac cgacgaacat tctgacttct gacttctggc ttctgacacc tgactcctgc
    11161 gcttatggcc atctcgctga agaccgagca cctcaaacgc tacaaggaca tcgcctggct
    11221 gctgatgaag tacgggcgct ccgacctcgt caagcaggcg gggctggagg aggcgctcac
    11281 cgaggagacg gagaaattta aagacggagt gccggccgag gcgcgcgagc tggccgaaga
    11341 tttcgagcgc atggggccga ccttcatcaa gctggcccaa ctgctctcga cccgctccga
    11401 cctcctcccc acgccctacc tcgaagccct cacccgtctg caagacaagg tcgagccgtt
    11461 cagcttcggc gaggtcgagg agatagtttc taaagagctg ggcgtgcgtc tgtcgaaggc
    11521 gttcgcggag tttgagtcgc agcccgtggc cgccgcctcg ctcgggcagg tgcatcgcgc
    11581 cacgatgcgc gacggccgcc acgtcgtcgt caaggtgcag cgccccaaca tccgcgagca
    11641 gatcgtcgat gacctcgcgg cgctcgccga gatcgccggc ttcctcgacc gccacaccga
    11701 ggcgggccgc cgctacggct tcgtcgtgat gctcgaagag ttccgcaaaa ctctgctgcg
    11761 cgaactcgac taccgcgccg aggcgcgcaa cctcaccacc attgcccgaa acctctccga
    11821 gttcgagcgc atcatcatcc ccgtccccct cgaagactac acgacctcgc gcatcctcac
    11881 gatggacttc gtgcagggcc gcaagattac cgacctcggc ccgctcgcgc agatggatgt
    11941 ggacgggcac gagctggccg acgatctgtt ccgcgcctac ctcaagcaga ttctggtgga
    12001 cggtttcttt cacgccgacc cgcatcccgg caacgtcttc ctcaccgacg acggctccat
    12061 cgccctcatc gacctcggca tggtcgcccg cctcaccccg cgctttcagg agagcctgct
    12121 gcaaatcctc ctcgccatca gcgaggggcg cggcgacgac gtgtccgaca tcgccatcaa
    12181 gatgggcgag gcgctgcccg acttcaacga ggtgaacttc cggcgacagg tcggcgactt
    12241 agtcgcacag catcaggggt cgagtctgga taacatggac gcgggccgtg tcggactcga
    12301 aatcacgcgc gtcgccgggg agaactgctt ccgcctgcct tcggacttca cgatgatggc
    12361 caaggcgctc ctcaatctcg accagatcgt caagacctta gaccccgtct tcgaccccaa
    12421 cgacgccatc cgccgccacg cctccgagat gatgcgcgag cgcatgatga agagcctttc
    12481 gccgggcaac ctcttcatgt cggccatcga ggtcaaggac ttcgtcgaga aattcccggc
    12541 ccgcgtcaac cgcctgctcg acgccgtggc caacaacgac gtgcggatca agatcgacgc
    12601 catcgatgag acccgcctca tggaaggctt ccagaagatc gccaaccgca tcaccgtcgg
    12661 cctggtcatc gcggccctga tcgtcggggc cgcgctcatc atggacatcc agaccagctt
    12721 tactttgttc ggctaccccg gcctcgccat catcttcttt ctcatcgccg ccggcgccgg
    12781 tctcatcctg atttacaaca tcctcttcaa agacgagaag aagaaagacg acgggccgaa
    12841 cgccgcgccg tagattcggc gctgacactt gtgaccacga cgtgaaacca atggagagcc
    12901 tatcgcctga gacgctttac gaacgcgccg agcacgccgc gcgcgtcatc cgctcacgcc
    12961 gcgccgagga gccgcgcgcc gcgcttgttt taggtagcgg tctcggcgct ttcgccgacg
    13021 acctcgaaga ctcgctcgcc atcccctacg aggagatacc gggatgtgcg cgctcgaccg
    13081 ttgaaggcca cacgggacgc ctcgtgctgg ggcgcgtcgg cggcgtcgag acggccgtga
    13141 tgcaggggcg cttccacttc tacgagggct acacactcga agaggtgacc ttccccatcc
    13201 gcgttctggg cttgcttggt gttaagtcgc ttgtcctgac gaacgcggcg ggcggcctca
    13261 acaacgccta caccgagggc gcgctcgtct gtatctccga ccacctgaac ctgatgggcg
    13321 tcaaccccct ccagggccgc aacgatgaac gcttcggccc gcgcttcccc gacatgacgg
    13381 ccgtctacga ccacgagtat caggagacgg ccatccggga ggcgcgtgcg tgcggccttg
    13441 agctgcggcg cggcgtctac gcggcactcg ccggcccgtc atatgagacg ccggccgaga
    13501 ttcggatgct gcgcctgctc ggcgccgacg ccgtcggcat gagcaccgtc cccgaggcca
    13561 tcgtcgcgcg gcagatgggc ctgcgcgtgc tcggcatctc ctgcatcacc aacctcgccg
    13621 ccggagtgct cgaccgcccc atcgaccacg ccgaagtgat ggaaacgggc cagcgcgtcc
    13681 gcgagaagtt ctcggagctg ctgcggcgag taatacctaa attgatgtga taagtgatga
    13741 gtgataagtg ataagaaaaa gcgtggcccg cttctttctt atcacttatc actcatcact
    13801 tatcacgtca tcggagcgaa gcgacgatga tcatcggcat ttgtggcggt acagggtcgg
    13861 ggaagacgac cgtggcgaaa cgcatcctgg agtcggtgcg cgcgtgcgag gtagtcttcc
    13921 tccaacagga ctcctactac cgcaacctcg ccgacctcgc gctcgtcttt cgcgagacgg
    13981 tcaacttcga ccaccccgac tccatcgaca acgatctgct ggtcgaacac gtccgcgagt
    14041 tgaaagcggg gcggtcggtg gagctgcccg tctacgactt caagacgcac acgcgccggg
    14101 cggagacgct gcgcgtggag ccgaagccga tcaccatcat cgagggcatc ctgattttcg
    14161 ccgatccgcg cctgctcgcc gagatggata tcaaggtctt cgtggacacg cccgacgaca
    14221 tccgcttcat ccgtcgcctc cggcgcgacg tggccgagcg tgggcgcacc gtggagtccg
    14281 tcatcgagca atatctaacg acggtacgcc cgatgcacat gcagttcgtc gagccgtcga
    14341 agcgcgtctc ggacgtgatt atccccgaag gcggtcgcaa cctcgtcagc gtcgatctca
    14401 tcagcggccg aatccgcgag cgtctgggcg aagagcgccg ccgccccgag gcggccgcgg
    14461 gcgcataatt atcaaagcaa cgaaagaagg gaagtgagca ttatcgaaga tcaggcatta
    14521 attgacgcgg ccacgtcggc gcgcgactgc gcgctggcga cctactcgaa cttttgcgtg
    14581 ggcgcggcca tcgaatcgga cgacgggcgc gtgtttacgg gttgcaacgt cgagagcgcg
    14641 gcctacgagc taacgatgtg cgccgagcgc gtgaccatct ggaaggcgct ctcggaaggc
    14701 gcgcgtcgct tcacgcgcat cgccgtcgtc gccgacacgg agcgcctgac gccgccgtgc
    14761 ggcgcctgtc gccagctcgt ctgggagttc tgcggcgaca tccccgtcat cctcgccaac
    14821 ctgcgcggcg acacggagac cctacagatg agcgagcttc tgccgcgccc gttcgacgcc
    14881 aagtttctcg aagaggccag cacgcaaagt taaggaggcg gcacgcgatg tcagacaagg
    14941 agaaggcgga cgaggccgaa cccgagaagg ccgaaggcgg aaaggccgaa ggcggcgcgg
    15001 agacggtcgt cgagcgcgtc atcaaccgct acatgggcga cacgattcac ctgttcctct
    15061 ccctgctctc gatcctgatt atcgccgccg ccgtgctcgc cacctacgag acgctcgtcc
    15121 gcgacttccc gaagctcctc gcgcccacgg acgagtacaa agtcttgcag gacatcatcg
    15181 gcaacatcct gctgatcgcc atcgcggccg agttaggttt gctgctgctg ttccaccgga
    15241 cgagcgccgc cgtggaggtc atcatcttcg tcatcgcgcg caagatcgtc agcccgaacc
    15301 tctcgtccct cgacctgctg ctcgccgtgg ccgccctcgc cgggctgctc atcgcgcgct
    15361 tctactatct gcccggcaag cccaagtagc ttaacgaaaa agtaatttac cgaaaggcga
    15421 gatcgtcaaa gtgtcaaagc tcagtcgaag actcttctgc gtcgtgttgg ccggcgtcgt
    15481 cctgtggccg ctcatacccg cggaggcgca gaggcgcagg gcgtctgccg ctacgacggt
    15541 ggtcgatatg ctcgtggtcg gcggaacaat cgtcacaatg gatgagcgca agcgcgtcat
    15601 cgaggacggg gcggtcgccg tcgcgcgcgg gcgcatcgtc gccgtcggag cgcgcgccga
    15661 ggtcgagcgc cggtacaggc tcgcgccgcg cggcgagcgc atcgacgccg cgggccgagt
    15721 agtcatcccc ggtctcatca acggccacac gcacgcgccg atggtgctct tccgcggcct
    15781 cgccgacgac cttgagttga tggactggct gacgaaatac atcttcccgg ccgaagccaa
    15841 aaacgtcacg gaggagttcg tccgcgtcgg cacacgcctc gccctcgccg agatgattcg
    15901 cggcgggacg accacctact gcgacatgta ctacttcgag gacgccatcg cggacgagac
    15961 ggcgcgcgcg ggggtgcgcg gcgtgctggg cgagacggtc atcgacttcc ccgtcgccga
    16021 caacaagacg tgggccgagg cgatggccta cagcgaacgc ttcgtcaaaa agtggaaggg
    16081 ccatccgctc atcacggcgg cggtcgcgcc gcacgcgccg tacaccgtct cggaggagca
    16141 cctgaaggaa gtgcgcgcgc tcgccgaccg cacgggcgcg cccatcgtca ttcacctcgc
    16201 ggagacgcgt caggaagtcg ccgacatcac gaagctcaaa ggcgtgccgc cggccgagta
    16261 tctcgcgcgc atcggcttcc tgcgccctga cgtgatcgcc gcgcacaccg tccacctcaa
    16321 cgagcgcgag atcggtctgc tgaaagagca cggcgtcggg agcgttcaca accccacgtc
    16381 gaacatgaag ctcgcgtccg gcgtcgcgcc cgtcatgtcg atgctgaacc gcgaggtgag
    16441 gctcggcctc ggcaccgacg gcgcggcctc gaacaacgac ctcgacatgt gggaggagat
    16501 ggacctcgcc gccaagctcc acaagctctc ggcgaacgac acgcgcgtgc tgccggccga
    16561 tctcgcactg gcgctcgcca cgctcggctc cgcacgcgcg ctgcacatgg agaaggagat
    16621 cggatcgctt gaggagggca agcgcgccga cctcgtcgtc gtggacatcg acggcctgca
    16681 tcagacgccg ctctacaacg tctattcgca cttggtctac gcgacaaaag cttcggacgt
    16741 gcgcacggtc gtcatcgagg ggcgcgtcgt catgcgcgac cgccgcttgt taacgcttga
    16801 cgaggcagta gttaaacgtc aggcgcgcgt ctttcgcgaa cgcatctcca acagtctcaa
    16861 gtagttgtcg aggcgcccga gagcggcccg acggcgtcta acgggttgct taaatggggc
    16921 cgtgcgggcc ataatacgca ccttcaagcg ggtcagagcc tacaggcgag cgctcgttcc
    16981 ggctcgcctt ctcaacagag aaaaattagg agtaagacca cgtgaaggga acccgtatac
    17041 ttcgcggagc acgcttcgcc atcaccgcct tcagcctcgc tctgattttc gccccccaca
    17101 cagacttgcg cgcgcagcag gcgcccgccg aagaagcccc ggaggccgac gttgtccagc
    17161 gccgcgtgac gcgcgcccgc gccctcgccg ccgtcggcaa actcgccgcc gccgctcagg
    17221 atttagaaca gttgaggacg accgccgcgg acgactccgt gcgcgacgtg gccagcatcc
    17281 tcctgatgaa catctacgtc gagatgccgg actacgcgcg cgcccacacg ctgctcgaag
    17341 aggctttcaa gggccgcgcg tcgcgttcgg aagctgccgt gcgttcccac tacgcgctcg
    17401 cggggcagac cgtgggcagc gtccgcgcac acgtcgagcg ttaccgctcc ttcggcatca
    17461 acgtcgtgga cgaagacctg ccgaccgagg ccaagtctga tcttgaacag atgcgcgcgc
    17521 tgctggaacg tgtgtgggag caggccaaat ccgtgcgcga ggaagagcag aaaagcggca
    17581 gcggcgaccg gggtgcggat gccacggcgc tcatcgagga ggcggccaac gtccgcctgc
    17641 ggctggcgcg cggtgatgag gagcgcgcgc gctggcggca ggaagtttcg gaagcgcgcc
    17701 agcggctcgt cgccgccgag acgcgcatca cgaaggtgag cgacattgtc tccgccgccg
    17761 cgccggccgc gggcgtctcc gcgaacaacg tctacgtgcc gccggcttcg acgactggcc
    17821 ccgcccccgc gcagcccgcg ccgaaggagc gcgccgcgaa cgcctcgaag aagacggcgc
    17881 ccgccgcgtc gaatgataat gcgccccggc cggagttgag ggcggagagc gcgaagccgg
    17941 ccgacgccgc cgcgccgaag cagggcgggc tgttctccgt cgggtcgctg caaggtctgg
    18001 ccgcgaagaa ggtctcgcca agctacccga gtctggcgaa gtcagcgcgc atcacaggcg
    18061 tggtcacggt cttcctcgtc ctaaacgaga acggcacggt tgagtcggtg cagcgcgccg
    18121 acgggccggc acaactacag accgcggcca ctgacgccgt ccgccgctgg aagttcagac
    18181 agaccctcat cgacggccag cccgtgaaag tgtcgggtta catcaacttc aacttctcgc
    18241 tgtagtgtag gggctgacga cgcctaccga tcccgccccg cgagtttgag tcccggaggg
    18301 acggcagaat gtagcccacg gcgtgagccg tgggtaaagg ttaattacaa aatcgggagc
    18361 cccagagggg cgaaagaaaa tcgaggttga atatgctc
//
LOCUS       DASVES010000318         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_13857, whole genome shotgun sequence.
ACCESSION   DASVES010000318 DASVES010000000
VERSION     DASVES010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13857"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..1747)
                     /locus_tag="VF240_12975"
     CDS             complement(440..1747)
                     /locus_tag="VF240_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX7176167.1"
                     /translation="MTRNRTASLAEKMSPLFSPKAIIRRRARAVARFAVALFALALFA
                     PAALGQAAAPVYDTGVSFKLKSEVLGEERTILVRTPAGYDRSDARFPVLYMSDGDAHL
                     QHTAASVAFLARNNRMPEMIVVGVTNTDRTRDLTPTKVERMSGNPNARFPTSGGADKF
                     LKFIETELIPQIESKYRTSPFRAFAGHSLGGLFAVHAMVSRPELFNAVIAVSPSLQWD
                     NFVTIDRAKEFFKTRKDYNRTLFVTLGNEQGDIEDGYKLLKDILDKQQVKGFAWEAVH
                     MTDEDHGSVVMRSHYAALRKIYEGWQFPRNPMTGGMNVTGLKSVEDHYTKLSDRLSYK
                     IVAPEPLMNQLGYQLMGEGKMDEAIEAFKLNVERYPKSANVYDSLAEAHEKSGKLDLA
                     RSGYEKAVAIGKETADPNLAVYQTNFDRVSGLLSKGTTSEAKP"
     gene            2072..2959
                     /gene="mazG"
                     /locus_tag="VF240_12980"
     CDS             2072..2959
                     /gene="mazG"
                     /locus_tag="VF240_12980"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX7176168.1"
                     /translation="MPANFEDLVALMSRLRAPDGCPWDREQTYATLAPMLLEEAYEAF
                     DAVEDAREGRPLELRDELGDLLFQIVFYAQVASERDEFRIADVIEQIHTKMVRRHPHV
                     FGDATVRDNEELLRNWEAMKAEEKRAAGKEAKAEDASLLDGVSARLPALMESHQLATK
                     AARVGFDWQNVEDIFDKLHEEIEELREAIREHGDTQTKAAGDADADVPAGGDAAPRAE
                     ANDTRMREELGDVIFAVSNIARHLGVEPEAALKLTNRKFRRRFRHVEHGLHAAGREPG
                     SATLDEMEALWQEAKKSDK"
     gene            3129..3824
                     /locus_tag="VF240_12985"
     CDS             3129..3824
                     /locus_tag="VF240_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176169.1"
                     /translation="MIQFVSNYEDLSTDRGYQFKFHCDKCGNGFMSRYQPSTLGTAGS
                     LLRAAGDIFGGWASSAGNSAYEMQRSVGGKAHDEALRKAVEEGKEHFHQCSRCGRWVC
                     PEVCWNASAGQCEGCAPDYTEEFASSHAQAKVQAARQQLDEKARATDYAAAADMSAGA
                     TESAPGAALAEQQQEATAAARCISCGAAMGKAKFCPECGTPAKAARPACSSCGHEPEG
                     VPKFCPECGTKMG"
BASE COUNT          670 a         1179 c         1313 g          678 t
ORIGIN      
        1 ctacgagaag gcggtcgcca tcggcaagga gacggccgac cccaacctcg ccgtctatca
       61 gacgaacttc gaccgcgtct cggggctgct cagcaagggc accacgtccg aggcgaagcc
      121 gtaaccgccg cgacgagcga cggcccggcg cacgcgcgga atagactaag ggccagcccc
      181 gtcaggggcg acagcagtgt tgcccacggc gtgagccgtg gggacaggcg ccagaaaaca
      241 ttagaagccc tggaggggcg aaagaatcgc agacttgcgc gttgcgccgg tcgcgattct
      301 ttcgcccctc cggggctttt cgatttttct cgcgtctgtt ccacgggctg gcgcccgtgg
      361 ctacattctg tcgcccctga cggggctggc ccttagtcta ttccgcgctc gtgcgccggg
      421 ccgttgctcg tcgcggcggt tacggcttcg cctcggacgt ggtgcccttg ctgagcagcc
      481 ccgagacgcg gtcgaagttc gtctgataga cggcgaggtt ggggtcggcc gtctccttgc
      541 cgatggcgac cgccttctcg tagccagagc gcgcgaggtc gagcttgccc gacttctcgt
      601 gagcctcggc gagactgtcg taaacgttcg ccgacttcgg gtaacgttcg acgttcagct
      661 tgaaggcttc gatggcctcg tccatcttgc cctcgcccat caactgatag ccgagctggt
      721 tcatcagcgg ctcaggcgcg acgatcttgt agctcaacct gtcggagagc ttggtgtagt
      781 ggtcctccac gctcttgaga ccggtgacgt tcatcccgcc cgtcatgggg ttgcggggaa
      841 actgccagcc ctcgtagatt ttccgcaggg cggcgtagtg gctgcgcatg acgaccgagc
      901 cgtgatcctc gtccgtcatg tgcacggcct cccacgcgaa gcccttcacc tgctgcttgt
      961 cgaggatgtc tttgaggagc ttgtagccgt cttcgatgtc gccctgctcg ttgccgaggg
     1021 tgacgaagag cgtccggttg tagtccttgc gagttttgaa gaactccttc gcgcggtcga
     1081 tggtgacgaa gttgtcccac tggagcgacg ggctgacggc gatgacggcg ttgaacaact
     1141 cgggccgcga gaccatcgcg tggacggcga agaggccgcc gagcgagtgg ccggcgaagg
     1201 cgcggaaagg cgaagtgcgg tacttcgact cgatctgcgg gatcagctcc gtctcgatga
     1261 acttcagaaa cttgtcggcg ccgccgctcg tcgggaagcg ggcgttgggg ttgccggaca
     1321 tccgctcgac cttcgtcggg gtgaggtcgc gcgtgcggtc ggtgttggtg acgccgacga
     1381 cgatcatctc gggcatgcgg ttgttgcgcg cgaggaaggc gacgctggcg gccgtgtgtt
     1441 gcaggtgcgc gtcgccgtcc gacatgtaga ggacggggaa gcgcgcgtcg ctacggtcgt
     1501 agccggccgg tgtgcggacg aggatcgtgc gctcctcacc gagcacttcc gacttgagct
     1561 tgaagctcac gccggtgtcg tagacgggcg ccgccgcctg accgagcgcg gccggggcga
     1621 acagagcgag cgcgaagagc gcgacggcga agcgcgcgac ggcgcgggcg cgacggcgga
     1681 tgatggcttt cggcgagaaa aggggagaca tcttttccgc gaggctggcg gtgcggttac
     1741 gggtcatggt tcctccgaag tactaggctg gcgtttttcc gggcttcgcc gtcacttgtc
     1801 tggcgagggc ttcggcgaag ggcttcatgt gcgtggcggc aagctgcggc cgacattcta
     1861 cagcaagagc cgcccgcgtg tcggccccac gcacctcaat ttacaaacac gagacaagtt
     1921 tttacttatc gtttacaaac gtcccaccgc ctgtaaacaa gtccaaataa ttagttcaac
     1981 gttcacagtg ggattgatgg cgacggcgct tgccctcagt tttcgaatct ctgaactttg
     2041 aactttgaac cttgaacttt atcctaacgt catgccagcc aactttgaag accttgtcgc
     2101 actgatgtcg cgtctgcgcg cgcccgacgg gtgcccgtgg gatcgcgagc agacctacgc
     2161 cacgctcgcg ccgatgcttt tggaagaagc ctacgaggcg ttcgacgccg tggaggacgc
     2221 gcgcgagggg cggccccttg agctgcgcga cgaactcggc gatttgctct tccagatagt
     2281 cttttacgcg caggtcgcct cggagcgcga cgagttccgt atcgccgacg tgatcgaaca
     2341 gattcacacg aagatggtgc gccgccaccc gcacgtcttc ggcgacgcga cggtgcgcga
     2401 caacgaggag ctgctccgca actgggaggc gatgaaggcc gaggagaagc gcgccgccgg
     2461 caaggaggcg aaggctgaag acgcctccct gctcgacggc gtctcggcgc gtctgccggc
     2521 gctcatggag tcgcaccagc tcgcgacgaa ggccgcacgc gtcggcttcg actggcagaa
     2581 cgtcgaagac atcttcgaca agctccacga ggagatcgaa gagctgcgcg aagccatccg
     2641 cgagcacggg gacacgcaga ccaaagctgc cggcgatgcg gatgcggatg ttccggcggg
     2701 cggcgacgcc gccccgcgcg ccgaggcaaa cgacacgcgg atgcgcgagg agttgggcga
     2761 cgtgatcttc gccgtctcga acatcgcgcg ccaccttggc gtcgagccgg aggccgcgct
     2821 caagctcacc aaccgcaagt tccgccgccg cttccgacac gtcgagcacg gccttcacgc
     2881 ggcggggcgc gaacccggct ccgcgacgct cgacgagatg gaagcgctct ggcaggaagc
     2941 gaagaagagt gataagtgat gagtgatgag tgatgagtga tgagtgatga gttggtgagt
     3001 tggtgagtga caaaatcggg gccgagatgc taagtttacg gggccggcaa tgggtcgccg
     3061 acggcgcttc ttcctcgtca cttatcactc atcactcttc accgctccgg gggttttgcg
     3121 agatgtcgat gattcaattc gtcagcaact acgaagacct gtccacagac cgcggctacc
     3181 agttcaagtt ccactgcgac aagtgcggca acggcttcat gtcgcgctat cagccttcga
     3241 ctttgggcac ggccgggtcg cttttgcgcg cggccggcga catcttcggc ggctgggcca
     3301 gctcggccgg caactcggcc tatgagatgc agcgttcggt cggcggcaag gcgcacgacg
     3361 aggcgctccg caaggcggtc gaggagggca aggagcattt ccaccagtgc tcgcgctgcg
     3421 ggcgttgggt ctgccccgag gtctgctgga acgcgtcggc cgggcagtgt gaggggtgcg
     3481 cgcccgacta cacggaggag ttcgcctcgt cgcacgcgca ggcgaaggta caggcggcgc
     3541 gccaacaact cgacgagaag gcgcgcgcga ccgactacgc cgccgccgcc gacatgagcg
     3601 ccggcgcgac agagagcgcg ccgggcgccg cgctggccga gcagcagcag gaggccacgg
     3661 ccgccgcgcg ttgcatctcg tgcggcgccg ccatgggcaa ggcgaagttc tgccccgagt
     3721 gcgggacgcc cgcgaaggcg gcgcgccccg cctgctcttc ctgcggccac gagccggagg
     3781 gcgtgcccaa attctgcccc gaatgtggca cgaagatggg ctgagcgctc gggacaaaaa
//
LOCUS       DASVES010000319         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1595266, whole genome shotgun sequence.
ACCESSION   DASVES010000319 DASVES010000000
VERSION     DASVES010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595266"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VF240_12990"
     CDS             complement(<1..391)
                     /locus_tag="VF240_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7176170.1"
                     /translation="MSLEKYYGVLGLKPDASEQEVKNAYRDLAKVWHPDRFAHDPRLQ
                     QKAQEKLKEINEAYEAINSPASGRRHRARRPTPPPPSSSTSTSAPRPPRGGPADFTTV
                     VARRSDAGAGASRLTPVALAVAAVVITF"
     gene            783..2168
                     /locus_tag="VF240_12995"
     CDS             783..2168
                     /locus_tag="VF240_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX7176171.1"
                     /translation="MVLTTAAAATAAANAKYPPGPKRRLPGGQMFAFRRDPLKFLTHI
                     AGEYGDVSRFRVGPQVFYFLNHPDLVRDVLVTHHERFHKGRALQRAKRLLGEGLLTSE
                     GEHWQRQRRLAQPAFHRQRLQGYGRVMVDYAERAAERWRDGEALDISEEMMRLTLSIV
                     GKTLFDADVEHDASEVGGALSEIMDLFGYLMFPFAELMEKVPILPPVRRFNRARARLD
                     GVVYRIIDERRRGGEDRGDLLSMLLLAEDEEGDRTGMSNEQVRDEAMTLFLAGHETTA
                     NLLAWTWYLLSRNPEAERRLHAEIDAVLEDGRAPTVDDLPRLRYTEMVVSEVMRLYPP
                     AWAVGRRAIADHEAGGYHVPANSLILVSQYVAHRDPRFWPDPERFDPERWTAEARGQR
                     PQFAYFPFGGGPRRCVGEAFAWMEATLILATLASRWRMRLAPGSTVETSPRITLRPGR
                     GGVPVTLERRR"
BASE COUNT          389 a          767 c          816 g          397 t
ORIGIN      
        1 aaaatgtgat aacgaccgcg gccactgcta acgcgaccgg cgtcaggcgt gacgcgccgg
       61 caccagcgtc gctgcggcgt gcgacgacgg ttgtgaagtc ggcggggccg ccacgcggcg
      121 ggcgcggggc ggaggttgac gttgacgatg acggaggagg aggcgtgggg cggcgcgcgc
      181 ggtgtcggcg gccggaagcc ggggagttga tcgcctcgta tgcctcgttg atctctttga
      241 gcttctcctg cgccttctgt tggaggcgcg ggtcgtgggc gaagcggtcg gggtgccaga
      301 ccttcgccag atcgcggtag gcgtttttca cttcctgctc ggaggcgtcg ggcttgaggc
      361 cgagcacccc gtagtatttt tcgaggctca tcgagtcttc cattgaatct tgggcgcggc
      421 cgttggggcg tgggctgtgg cgtggcgtac aaacaatgag gcgccgaagc ggggcgtcga
      481 gacgctttcg gccgacaaat taaagttaca ggccggcgcg cgcgcgtgtc aacgtttagg
      541 gcaaatttcg tttgtgcgca gggcgtgcgt cgagtgtgcc gggtggtcaa gagggaacac
      601 ggattaaact gatgagcacg gctcggcacg aatccgtgct catccgtttt catccgtctt
      661 atccgtgttc cccggcgacc ggggcgtcta actttgtgtt ttcgacgagc cgggcgcatc
      721 cttctcgcga gcgtcaagag tcggcgttag ttggaatcga cgaggcaggg agttttcaaa
      781 gaatggtttt gacgacagcc gcggcggcga cggccgccgc caacgcaaag tatccgcccg
      841 gcccgaagcg acggctgccc ggcgggcaga tgttcgcctt tcgccgcgac ccgctgaagt
      901 ttctgacgca tatcgccggc gagtacggcg acgtgtcgcg cttccgcgtc gggccgcaag
      961 tcttctactt cctcaaccac cccgacctcg tccgcgacgt gctcgtcacc caccacgaac
     1021 gcttccacaa gggccgcgcc ctccaacgcg ccaagcgcct gctcggcgag ggactgttga
     1081 cgagcgaggg cgagcactgg caacgacagc gacgcctcgc gcagccggcc tttcaccgcc
     1141 aacgtctgca aggctacggc cgcgtgatgg tcgattacgc ggagcgcgcc gcggagcgtt
     1201 ggcgcgacgg cgaggcgctc gacatctccg aggagatgat gcgcctcacg ctctccatcg
     1261 ttggcaagac actcttcgac gccgacgtgg agcacgacgc ctcggaggtg ggcggcgcgc
     1321 tctcggagat catggacttg ttcggctacc tgatgttccc cttcgccgag ttgatggaga
     1381 aggtgccgat cctgccgccc gtgcgtcgct tcaaccgcgc ccgcgcccgc ctcgacgggg
     1441 ttgtctatcg catcatcgac gagcgacgcc gcggcggcga ggatcgcggc gacctgctct
     1501 cgatgctgct gctcgccgag gacgaggagg gcgaccgcac cggcatgtcg aacgagcagg
     1561 tgcgcgacga ggcgatgacg ctcttcctcg ccggccacga gacgaccgcc aacctgctgg
     1621 cgtggacgtg gtacttgctc tcgcggaacc ccgaggccga gcgccggcta cacgcggaga
     1681 tcgacgcggt gctcgaagat gggcgcgcgc caacggtgga cgatctgccg cgtctgcgct
     1741 acacggagat ggtcgtctcc gaggtgatgc gtctctaccc gccggcgtgg gccgtcggcc
     1801 gccgggccat cgcggatcac gaggccggcg gctaccacgt ccccgccaac tcgctcatcc
     1861 tcgtcagcca gtacgtcgcg caccgcgacc cacgcttctg gcccgacccc gaacgcttcg
     1921 accccgaacg ctggacggcg gaggcgcgcg gccagcggcc gcagttcgcc tacttcccgt
     1981 tcggcggcgg cccgcgccgc tgcgtcggcg aggcgttcgc atggatggag gcgacgctga
     2041 tactcgccac gctcgcctcg cgctggcgca tgcgcctcgc gccgggcagc acggtcgaga
     2101 ccagcccgcg catcacgctg cggcccggac ggggcggcgt gccggtgaca ctggaacgga
     2161 gaaggtaaac atcgcgccgc aaaaaagagg agcgcacaac cagccgcgga gcggcggaag
     2221 aatgtagccc acggcgcgag ccgtgggaat aggacgcaaa ataattcgaa gccccggagg
     2281 ggcgaaagaa tctcgacgtt gagcgtgatg cccgtcgaaa ttctttcgcc cctccggggc
     2341 ttaccaaatt ttctcaccgc tgttccacg
//
LOCUS       DASVES010000320        47276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_201694, whole genome shotgun sequence.
ACCESSION   DASVES010000320 DASVES010000000
VERSION     DASVES010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 47276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201694"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VF240_13000"
     CDS             complement(<1..254)
                     /locus_tag="VF240_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7176172.1"
                     /translation="MDAEEELLDKPRAVRAGEQLNTDALLAYLRARLPGVEGPLVVEQ
                     FPAGYSNLTYLVRAGAQEFVLRRPPFGSKVRTAHDMGREYA"
     gene            complement(278..1483)
                     /locus_tag="VF240_13005"
     CDS             complement(278..1483)
                     /locus_tag="VF240_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7176173.1"
                     /translation="MSHPEDRLQSLLSQIREFIIAEAIPLEQDFARRPFRELLPRLAA
                     LRQKVKELRLWAPQIPSEHGGLGLTLMEFARVSEELGRTPLGHYLFNCQAPDAGNMEV
                     LMSHGTPEQKETFLAPLARGDVRSCFSMTEPEHAGSNPVWMSTTAARDGDDYVINGHK
                     WFTSGAEGAAFAIVMAVTNADEANPYKRASQIVVPTDTPGFRIVRNVSVMGEEGSDYA
                     SHAEVRYEDCRVPRRNLLGREGEGFRIAQERLGPGRIQHCMRWVGVCERAFEMMCERA
                     ARRELAPGVPLGSRQSVQEWIAESRAEIKAARLLVLHTAERIDREGAAAAREDISLIK
                     FFVAGVMHRVLDRAVQTHGALGLTDDTVLAYWYRHERAARIYDGPDEVHKSVVARSVL
                     RKYGVDISV"
     gene            1754..3121
                     /locus_tag="VF240_13010"
     CDS             1754..3121
                     /locus_tag="VF240_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX7176174.1"
                     /translation="MRINHPDPRTRLLAALLALACLAACAPASAQEETAARALHAEWQ
                     RQRVADLDPALVKHADLQKYLATLRARGVPVTEVGRSLAGREIYQLEFGRGPLKVFMW
                     SQMHGDEPTATSALVDLFAYLHANRQLPWVKAVEEKITLRAVPMLNPDGAELFTRRNL
                     QSIDINRDARTLTTPEGRLLKKLRDDWQPEIGFNLHNQNSRTAVGDTLKQAAVSLLAV
                     VSDAAGTDTPGRVRNKRLCAVMVASLAPFIEGHVGRYDDEFNPRAFGDMISQWGTPVI
                     LIETGALRGRTNQDLVGLNFVAIASALRALADGSYERADPKVYDALPMNGGGIYDLVV
                     RGAHVVNRVREGKTTTAPPFATDLAVNIDAGRDGRARRASVQDIGDLSVFRGLSEVDA
                     REYYVTVPTGGMLRNGGEATLLFYPKTQAARLDWDAPDFETKFPPEAVFRGGEWVKRG
                     RLPIK"
     gene            3118..5178
                     /locus_tag="VF240_13015"
     CDS             3118..5178
                     /locus_tag="VF240_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7176175.1"
                     /translation="MRRAHTRTPLAVFVLLASLLLQGAARGRVVTPPQGSSPAEVVER
                     GKFRIYKLQYPVGEESYEIAREGGAHVLSAKFEINYLGDKVPLTATLRTRSADLAPLR
                     FEIKGRTSTRSEANAAVEVGEGGAATVREGTKTRTEQAAGARIFPSGGLLPVSLQQAL
                     FQYAARHGALRNLRTLPVGRVSFEPRGDEQVEAGGRRVGLTRYSVSGLVWGRETAWFD
                     EGRRLIALISPDAELDRLEAVREGYEHLLPLFVARAAADSVADLRKISDAVRPLRAGR
                     YAIVGATLIDGTGTPPVADAVVLVENGRITAAGPRASVKIPKGVNVFDARGKYLLPGL
                     WDMHAHAEQGEWIPASLAAGITTMRDAGNEPEFILPLRDALRAGKVAGPRLLLAGIID
                     SPPGALGNNVAETPEAARAIVGRYKRDGYEQVKIYQSLKPELVSVVAAEAHRLGMSVT
                     GHVPTGVDAYAAVEAGMDQINHVGFILRAMRGRGWRPTPGTPPPPLNLDSPEAAAAIK
                     FFKERGTVIDPTLARGELNGHALDMPFVNFEPGMAKTPEELASILNNTGLPPEVAPRA
                     AASAALAARLTVMLMRAGVPVVAGTDLVVPGHTIYRELELYVRAGMTPAEALQTATII
                     PARVMRLDKELGTIERGKFADMILVDADPLADISNLRRVRYVVTGGRLYESAPLWRSV
                     GFRP"
     gene            complement(5312..6232)
                     /locus_tag="VF240_13020"
     CDS             complement(5312..6232)
                     /locus_tag="VF240_13020"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX7176176.1"
                     /translation="MRISLKTKRARWLAAAALVAAVVAGLYVALALREGRPVAEHEYF
                     GGVRRSLIIAHRGGAGLWPENTLHAFERARAAGADVLEMDVRSTADGALVVHHDETVE
                     RTTDGRGRVSELTLAEVKRLDAAHRWTPDGGETFPLRGAGHGVPTLEEVLARFASARF
                     VIEIKQDEPALAPPLCRLLREHAVEKRVLVAAFRQATLDDFRRACPEVATSATLSEGY
                     KFLALFKAGLGDTYRPAMQALQVPEHLRGWRVVTREFVETAHRLNLQVHVWTINDAGD
                     MRRLLDIGVDGIITDYPDRLAALRDQLSPR"
     gene            complement(6409..7464)
                     /locus_tag="VF240_13025"
     CDS             complement(6409..7464)
                     /locus_tag="VF240_13025"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX7176177.1"
                     /translation="MQTDFIIYGANGYTGALVARLAAERGLRPTLAGRSAEEVGRLAS
                     ELKLEHRVFDLSDAAAVDSALKEAAVVLHCAGPFSRTSKPMADACLRTRTHYLDITGE
                     ITVFEGLAARDAEAREAGVMLLPGVGFDVVPSDCLAAHLKRRLPAAVHLTLAIKGSGR
                     LSRGTATTMTENIDRGGLIRRGGKLTPVPAAWHTREIDFGKGPEPATTIPWGDVSTAF
                     HSTGIADVEVYAAMPPSMRWAMVVSRHVGALLASPWAQRYMKSRIKSGPPGPSDAERA
                     RGKSIVWGEAEDAAGKRCVSRLQGPEGYTLTAMTALAAVERVRAGQAPAGFQTPSRAY
                     GADFIMEIEGVTREDLE"
     gene            7732..7947
                     /locus_tag="VF240_13030"
     CDS             7732..7947
                     /locus_tag="VF240_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176178.1"
                     /translation="MRRVMLAVAGLLLLAPAPDARAQTADVIKDFDAWALREMSGLNA
                     ALAAKQLQPVPMLTREEWEKTDGQIKG"
     gene            8124..8570
                     /gene="mscL"
                     /locus_tag="VF240_13035"
     CDS             8124..8570
                     /gene="mscL"
                     /locus_tag="VF240_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX7176179.1"
                     /translation="MFKEFREFINRGNVMDLAVAVIIAAAFGKIITTLVEGVIMPPIG
                     LVLGRVDFASLFYTLDASKGVPVSLADAKARGVPVIAYGQFLNDVVGFLIVAFVVFLI
                     VKQVNRLKSKQDTDEGPTTKDCPHCLSTIPLKATRCKDCTADLRAA"
     gene            8704..9765
                     /locus_tag="VF240_13040"
     CDS             8704..9765
                     /locus_tag="VF240_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176180.1"
                     /translation="MKLFGLAITRPFSILLSLLCLLSQAEGLRSQTRSGVVRAGAGRA
                     SVEILDSDRGKDGLLGPVRRVRTEVARLDTGVTASAEGPRSLLEITVYDVGGKRIANR
                     THPVAVAGAGGGGETYEYDERGQLQATVVKDEAGAFLSRTVYSYEYDTAGNWVKMTAS
                     VAVSEAGAEMLEPVEVTYRVITYYSRGAGKAPANAVAAPASAGDSRAVNAEGAAEGLR
                     PTTSPRSSKAASDRHDGADGTDGASVPSAPSVEVKAEPPAPMFDAGLINDKALSLPRP
                     AYLVGSSAQPSPVVVKVEVVVDETGRVISARGTEGPRVLWWAAESAARQAGFFPFREG
                     GRPVRARGLLEYTFPFEPR"
     gene            9852..10955
                     /locus_tag="VF240_13045"
     CDS             9852..10955
                     /locus_tag="VF240_13045"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX7176181.1"
                     /translation="MREARFMVLTGHLDEQPLPGLIRTLHAQHKTGRLQVDYAESPAA
                     FHFEQGSLVDARLGALRGLEALMLALSLPGAPFNFNPLVKPPERTVEERERQFIRELL
                     DAAPAGTALDVPASSALPAEAETTTPRVVPLRGAALPPAAGERLALPAPADESLVYEV
                     NAALAAHSRRFSRERAAYAAVIALLLLLTFLSRLRNPVAPAAPPSPPQAVQQTAPDAA
                     SPPADERLRPADSSGGADPADVPATARRDEPARVERPSRETRQSSPAGAASSRGAAPL
                     PVGQTQTSAEAGVGGAGGEPPAGRERAANARGESSVRILLRVERGRVLQAVVQNSRPG
                     MESYEALALRMARQRRYPADFNGQDTLQLKVRQ"
     gene            10981..12489
                     /locus_tag="VF240_13050"
     CDS             10981..12489
                     /locus_tag="VF240_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176182.1"
                     /translation="MLLVERKTLSLTVDEPARLELLDALRAAQFAPLIRTARRRSFEL
                     SLAAAGGLPPAPELIDDFERQALSSRLRERAGPYTPGDLFCSEDAIFYLAFGDDAARG
                     HEAVVAGVVYDRESVDPLRRFELFCQRVRESMRLAGEGEAEAEAGELVAPPWESHDVR
                     RFPRGLTRYVARQDEEAARAMMFREGSRERLRAAELLADERVRAFLRREQEARVEGYA
                     PRLLSGESPSPEGLVPVEELVEAGLMRREVRVTCRKTRHPLFDLPTPDALALVTLSRA
                     RCSLCATEVADEVVEEVYNPTRLAAALLADGSWLRNRVYQIVRDLGVPESEIAAGPPA
                     EGGESHLVANVCGDAFLFALRDGELSPSFSRRVVEAAGEIEARHVIVIVTGAAEGEGR
                     MRLYEYAWRRAREGRDVDVAVGEGAAGARSCIEGVFERAVGGRLARDLCALDDALGMN
                     ASRLVLTKFKMRREQAGARRAETRRAARPAPTLPGQDGEESDAPPARLRLSS"
     gene            complement(12510..13724)
                     /locus_tag="VF240_13055"
     CDS             complement(12510..13724)
                     /locus_tag="VF240_13055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7176183.1"
                     /translation="MTLRLLLLAALLFDCGALAAAAARPQTQKPTPRVSSADAAARLR
                     RRLQEKLDALHAAGRFPGATAGFAFADGTSLGLSVGTSDTQKKTPMRPNDLMLQGSVG
                     KTYAAAVALQLVAEKKIGLDDKVEKYLGRESWFARLPNARDITVRMLMNHTSGLVRYE
                     FKEQFTKDLTANPDKVWKPEELVAYVLDTAPPFAAGRGWDYSDTNYIVLGMIVERVTG
                     RRYYDEVRRRLLGPLKLRRTVPSDARRIPGLAQGYAGADNPFGGTDAMIDSAGRFAIN
                     PQFEWTGGGIASTTEDLARWARALYEGRAFDRALMPQVLDGVAAKLGPGTKYGLGVII
                     RETELGVVYGHSGFFPGYLTEMAYFPDLKAAVAVQVNTSAPRSTGKPLFRFLTDFAAI
                     IREESQQAAAGR"
     gene            complement(13957..14823)
                     /locus_tag="VF240_13060"
     CDS             complement(13957..14823)
                     /locus_tag="VF240_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176184.1"
                     /translation="MELTSEAMGALRQYLLGQLAPEQRTPLEERLLTDAAFHDELSIA
                     EDDLIDRYLSGELAAADHQDFETHFLLAPERHKKLRFARALRRYVAAETDAPGSAVAL
                     ASLPAGLREEPAATKEQTPGDLGTRPATPGKQNLFQLLFARGPALAFSLAAALLLAVV
                     AVSWVAFDRPARREVAFRNPYAVTLTPGLTRGSGEPARIQLPAGADALRLRLELPAAE
                     HESYRAALLTAERAEVWAADELRPAAGSAAIDMDIPASVVGPGDYHVTVSGRAAGGRL
                     EDVSRYTFRVVR"
     gene            complement(14885..15451)
                     /locus_tag="VF240_13065"
     CDS             complement(14885..15451)
                     /locus_tag="VF240_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176185.1"
                     /translation="MKKNWSLTQEAFDRLLAWLGAGPEEAGRKYEEIRRSLIKIFTCR
                     GCHEPEDLADEVINRVAAKLPQIEADYTGDRALYFYGVANNVHLEYLRRRPAPPAPHP
                     VTAPAAEEEDERVYECLLNCVGGLSSHNRELVLQYYREEKRAKIDNRKHLAERLGIAL
                     NALRIRAFRIRLGLQKCVEECLTRPAAG"
     gene            complement(15520..19203)
                     /locus_tag="VF240_13070"
     CDS             complement(15520..19203)
                     /locus_tag="VF240_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX7176186.1"
                     /translation="MSFTKYVKYSLALLTIAFTLSEPTFKSRPRPPSLTAFAKSSSTE
                     HGAVSTLLPAPGKSTAQELSAGEVHSYQITLDTGQYLHAVVEPQGIDADVTLYEPGGQ
                     ALAQLDCRDYGPTPVSLIAETSGIYRLEVRSPEGQQTRGRYELRVEEIRPSTAEDRER
                     INAEKTFADGERLLKEWKAQSSRGAIDRFKDSLAFWKAVGDRRAEALTLKRIGDVYQP
                     LGEYQNALNYYRQALSLQREIKDRRGEGETLNELSFVYLTLGDNPKALNLCTQALKLN
                     RATGSRRGEAQALNNFGEVHYGLGKLQQSVEFYQQALPFWRELNDRKGQALTLLNFGY
                     TYSDLGQIGKAFDSYNQSLPLWQAAHDRRGQALTLNAIGRLYSRLGESQEALNFFESA
                     KQIIQPIKDPIEEARILNGMSFVYSGLGEKKRSLEYYNQALSLFRSVNYRSGEAATLY
                     ETGKVYHSLGDSQAALECFQQALSISKATSNHRLELYELREIGRIYDSWGWKDKALEY
                     YSRTLPFWQRENDLRGETDTLNLIGRVYEGRGQRQKALEYYAKALPLSRKAEYRAAEA
                     ATLHNIARMERDSGRLAEASARAEEALGVVESLRGKVVSQDLRASYFASVREQYEFYI
                     DLLMRLHRERPSEGFAAAAFEASERARARSLLETLSAARVGLRRKADPALLEREGALR
                     KELNDRAEQRRARLQTGGEQAEASALAKEIDELSAQLREVGAQIRAGSLEHTASLQTQ
                     PLGLKAIQERVVDDSTLLLEYSLGEERSYLWAVTKGTIHSYELPGRAEIEDAANRVRS
                     LLVAPQPVAGESARERQARVAQAEADYGREAERLSDMIIGPASEHLTGRRLLVVTEGA
                     LQYIPFGALTEPGTEASAGGAQERPEARPEGSARVPLFLRHEVVSLPSASILPTLQGG
                     AARYEQAGRSVAVFADPVFDKDDARVRGADSSRPDAAPPHASADAVAHQGKQEVHRAI
                     RDVSALFGGEGVPRLLASREEAEAIAAALPRGEVALVTGFGASRAAAVEPGLGRHRVI
                     HFATHGILDSERPEMSGIVLSLVDERGEPQDGFLSLQDIYNLDLSADLVVLSACNTAL
                     GREVRGEGLIGLTRGFMYAGAGAVVASLWKVDDEATAELMKHFYAGMFQQGLAPPAAL
                     RQAQLAMWRQKRWRAPYYWAAFVIQGQYGELKGMGDGPAYFAQWGLAAWGGGAAAISF
                     AALGAIRLRRRRRNSAGARQVKA"
     gene            complement(19706..20929)
                     /locus_tag="VF240_13075"
     CDS             complement(19706..20929)
                     /locus_tag="VF240_13075"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HEX7176187.1"
                     /translation="MKERQWTVMVYMAGDKHLDNNGFADLKEMKEAGSTAEVALIAQF
                     SRGVKHRPTKRYYLRKDGRDGALAGDVVEDLGETDTADPRSLEAFVRWGVENYPARRY
                     MLVMWGHGNGADDENVPNAASHPHRPDADEGCGQPTRGIGISHAAVLDSDTVDFLDTR
                     KFKQALDAAREIIGREVDVLGMDACLMSGAEVCYQVRGGARFTVAPEGVAPLDGWPYN
                     RILGELVARPEMEPERLACLVAEKYLASYADYEDVSVTQAVCDLGKCDALARSVDALA
                     GALLERLPEAGMIKSIVLSRWQAQSFDGTEYVDLYDFCSLLRENSDHAEVRSACEEVM
                     KTVGPDEFVVKSVYKEAALQYCYGLSIYFPQKEVSRTYQNLDFVKDTRWGAFLDAFVS
                     TTRRPDRAGPQGQGR"
     gene            complement(20976..22721)
                     /locus_tag="VF240_13080"
     CDS             complement(20976..22721)
                     /locus_tag="VF240_13080"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HEX7176188.1"
                     /translation="MCEGTKHKWTVMVHLAGDNNLSAECIFVLTEMCRVGTDDDIAVI
                     AQLDTGLHENTRLVIDFDTKPGNLNAKLNRGRKGQEEDESRRARARERSQARVAAKRP
                     GAAKRSGVVIDEESAEEKPPSYSEGICNFVKHCIRNHEAEHYMLVLSGHGGGILGDFL
                     DKDLADARDRLSVISMAKLIERVNEDLLGGRKIDILGLDSCLMSMTEVAYSVHNHAKV
                     MIGAEGFEPLTGWPYASVLETVRDFAAGDESGDDDYYVKELSRKIVCDYINYYSDYRA
                     AAVSVDQAACDLGESVHLMAAVKSLSRTLRGYLPDPKTGEGETDPEVRRALLVSHWEA
                     QPYKREQYTDLYDFCQLLKANCEYEDVQTACQNVMNTIRGSANEPEAAKADAAFRSEF
                     EGREYTDNFREMPGFVLKSCYSGLTVQYSYGVSIYFPWADVPADLNDYQKLYFATNSE
                     WALFLRAYLAATTRAARPGRGEPQSFSRRATIDKYNVASGSQSILLGHRNSSVDKNSS
                     VDKNSSVDKGLSNVIGTMRNPPTEFFECEDCDADAPSGAEPKEKTGARRDKAVKIQKP
                     SGRRINKSSGGRVKR"
     gene            complement(23185..23511)
                     /locus_tag="VF240_13085"
     CDS             complement(23185..23511)
                     /locus_tag="VF240_13085"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HEX7176189.1"
                     /translation="MRISFDRAGGFAAPAMRRSFKVDTDELAADEAEELRGLLAEADI
                     PSAASRQNAERAGAARPRPDAFHYRLVVEDDEDTRTVVASDADMPESLRPLVQWLTKR
                     STPGDS"
     gene            complement(23728..24876)
                     /locus_tag="VF240_13090"
     CDS             complement(23728..24876)
                     /locus_tag="VF240_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX7176190.1"
                     /translation="MAQSKDATVREAALRTLVTSARVRGQREIVGRVRAALTTNPPGE
                     KTRVIHDAEGKFLNEFELPGKLVGDESNPPSGGGAAAQAFDGLGATYDFFSEVLDRNS
                     IDDRGMRLVATVHFGAGYNNAFWNGRQMVFGDGDNIIFKGFTRSLDVIGHELTHGVTD
                     YTAKLVYHKQPGALNESFSDVFGSLIKQYAADPKQTADEADWLIGAGLLADGINGVAL
                     RSMKDPGSAYDDPQLGGRDPQPKHMSDYRNMPDDEWNDWGGVHINSGIPNHAFYLVAR
                     QLGGYAWEDAGLIWYNALLQLWEMAQFQDCANITAQVAGAVFGAGSAQQQAVREAWAE
                     VGINITAPAATKSRGKGGTKPPYESNGAALKKQLEQMGEVFRRAAEVA"
     gene            complement(25121..25663)
                     /locus_tag="VF240_13095"
     CDS             complement(25121..25663)
                     /locus_tag="VF240_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176191.1"
                     /translation="MATIWEKIKDNFTTLFVTTLVALLTVFSDSIVGNIKFAVNRANL
                     RTTHYEKLATDLSNYTFSAENVTEFYEQGWTSKSSLELAVKPYNDAIVNLRKQEYVNA
                     ALLHRFWDADDVSRFKAVMETVREVDTQIHSLNSEAEAVANAVKPKADPAVTKPVTDK
                     LKPLIEELKSRVSAFLSDLN"
     gene            complement(25729..26166)
                     /locus_tag="VF240_13100"
     CDS             complement(25729..26166)
                     /locus_tag="VF240_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7176192.1"
                     /translation="MAGMREIDFVFFDIGGTLGERDAATGRLVAFPSSARLLRMMREA
                     VGVRVGVITTLGPGLTNEDGKTLLRRAGLEEFIDPELFISDHDAGVAKPDAEIYRLAA
                     TRAGVPVGRCLFVGENLIEVIGALAAGMKAVLKPCPPGRDLPV"
     gene            complement(26235..27659)
                     /locus_tag="VF240_13105"
     CDS             complement(26235..27659)
                     /locus_tag="VF240_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176193.1"
                     /translation="MNGKLRWPALTRISLAACACLLWTVAAAGQPPPCTSANTVPVTI
                     ELAQALLDRDGSGRRILSVTFSESFDLSLPTNTPLDPLNVDPVVRANLNLDLERNPVK
                     DPSNYHLLDVRNSRRIPIVYVYFRTDRLTPNAVTLTVDPSVRLDEGDGVYHLYAFNLR
                     FISTSGSTITNSGLCPAKQLQRPVSVEAAVQPAPPPTGKADDSESGEGLSLTAADGRE
                     DANFYVSGEVTRPSGESFFGTVDVLIDYPVKKVIENRVHSYGPFFELKASSDPGADPD
                     SLNFGFNSRFPLWRYRGPNQSVPFRRLILKNSPKIEAERDFGNVNFVHETRFTLLSKT
                     FLSGSTTFYLRPFAGLEIGKNFVSPLDEAEGRFLIRPLAGSTLNIIFPMARGPIQSIG
                     LEASYIRRWPLRKEIKFEEDDDGELQPVEIGTGPRDYATAKLNFDFSKLYNAFIGYEY
                     GSVPPSFKLVDHKMVIGLTFKAKR"
     gene            complement(27656..28165)
                     /locus_tag="VF240_13110"
     CDS             complement(27656..28165)
                     /locus_tag="VF240_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176194.1"
                     /translation="MTLLSVEAEEIVDACIRSAVGWDGLISGGNKLSQVGVVDDLAID
                     ALIDELVTNNQVGVPSKQHKIDDPESLDLKTGTTVAQTQVQVIANSEAVQVAAKAAKA
                     GAKKAGRGLAAPTRGAAPAKSSVRAGATKKKATKSGKGAAKKSAKKARKGGAKRAGKA
                     GAAGRGKKR"
     gene            complement(28261..28740)
                     /locus_tag="VF240_13115"
     CDS             complement(28261..28740)
                     /locus_tag="VF240_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176195.1"
                     /translation="MAAKAAGNTPPGQERADTLKGWTMMALTALFILFYGAALTGWLK
                     PPSDERMVARLEPIIFVIVGYYFGRLPAQQNEKTLKDEIGRQTQRADAIQHAKEQSQQ
                     SREALEEKLKNARAALVSAAPGATAKSLAEAHDKTGSPVRDDALRGSVVAALNILNS"
     gene            29136..29702
                     /locus_tag="VF240_13120"
     CDS             29136..29702
                     /locus_tag="VF240_13120"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7176196.1"
                     /translation="MPSPSEITKLLVGWSNGDQAALESLLPLVEGELHRLAHHYMGRE
                     HAGHTLQTTALINETFLKLVDQSRVRWQNRAHFFGIAAQIMRRILINYARDQRREKRG
                     GGALRVSLSEADAVWAEKSCELIALDDALKRLADFDERKSRVVELRYFGGLSVEETAE
                     VLGVSGVTVMRDWNMARAWLAREIEDGS"
     gene            29692..32283
                     /locus_tag="VF240_13125"
     CDS             29692..32283
                     /locus_tag="VF240_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7176197.1"
                     /translation="MEVKDWQHVESLFHAALGLRAGERAALLARECAGDAALRGEVES
                     LLAAFEGHRDLLEEPAFSLGLKVMSEDAVSASLVGMQVGPYKLMEALGRGGMGEVYLA
                     EDTRLGRKVALKSLSGRLVGDNWARRQLIREAQAVAMLDHPNICAVHGIEEQDGHSFI
                     IMQYVEGENLSRLISERPPDAKRALALALQVVGALAEAHAHGIIHRDIKPQNVMVNAD
                     GQAKVLDFGLAKLVRPQSALGAVDSPSQASQSGLVMGTVAYMSPEQLRAERLDFRSDI
                     FSLGTVLYELLSGKNPFARTSDAETISAILTCRPAPLTQATSEVSPELSRIVLKCLEK
                     DREQRYHSASELLYELSNLQTAGHTAPRGRAHFARPHFTSRATRAAAAIFLLVLLVST
                     FLWLSYARLTRVQNLAVLPIVNESGDASFDYLGDGLTESLIKKLSGLSKLRIKAFTTV
                     AVYRGREFDPRQVGRELRVDAVLMGRLVRQGESLVLQTRLVDAANGSQLWDAKYDVRL
                     EQVFELQNQISENVAAGLELRLGLEEKNSLAAHRASPEAFNHYMLGRYYWNNRNPENI
                     QRAIKHFEEAFVLEANYAEAHAGLADCYVFLASAAYGEMTTADAMRKAKGAANLALKI
                     DERLPEAHTSVGVVQLRYDWTWDEAEREFKRAIELNPDYAPAHYWYSNLLAITGRLED
                     AIAESERAKALDPSSPSVSLNRCRAFFLARQHDRAAACFNEMLDKEPGNERVQYILGL
                     VYQQKGMYEEAIKIHEKIAETNLRLAAGALGYSYGRTGRREEALKMLDKINELSKQKT
                     VPVMETAIIYVGLGDKNNAFAWLEKAYEERFASLTYLAVEPLFDSLRADARYHSLLRR
                     LNLPPAR"
     gene            complement(32336..33838)
                     /locus_tag="VF240_13130"
     CDS             complement(32336..33838)
                     /locus_tag="VF240_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7176198.1"
                     /translation="MRAEIDIVHNPFGTLPESSSEKWRGLCASYLPVKAEASIWRYSR
                     APAPADPEQGWKLHVSATILTANVVFERAAPFLSGRGVLFKAPATLQELERINSGLYH
                     GYSQIGKFITVYPQSDAEARLLAGRLHRLTRGLSAPAIPFDLRFRPDSNVYYRYGAFV
                     PLRIEHPDGTSTLAMRDPQGNLVPDLRESVEAKPAWVGDPLIAGWPRPEARAPESPLR
                     TSFRAFRSLTQRGKGGVYQAVDLTTQTPRLCVLKEGRRDGEPAWDGRDGHWRVRQEER
                     VLKLLRAGRIDAPRVYASFEAGGNYYLVTEFIEGESLQNILQGLKRRMSVSRVLRYGV
                     QLATLVSQIHSAGWVWRDCKPSNVMVTKKGTLRPLDFEGACPVDEPDPVPWSTPEFAP
                     PESRATGGGRARVCDDLYALGAVIYFLLTGRPPAASSSPLAKLRRNIPTGVSRLVEAL
                     LSADPRRRPCAQMVAERLKALGPPPRSATRPVLAGRGSSVGRKRGVEEGV"
     gene            complement(34038..34355)
                     /locus_tag="VF240_13135"
     CDS             complement(34038..34355)
                     /locus_tag="VF240_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176199.1"
                     /translation="MTIKSNIEEVITKIIAEVDAGLEDKEVATEVGDKAVAAILGGAA
                     EWAAYMKLFAKTPEELARLIPTDGTADETKQKARAYLVANGTCGSGTTRGLLHTVENK
                     LNL"
     gene            complement(34471..35982)
                     /locus_tag="VF240_13140"
     CDS             complement(34471..35982)
                     /locus_tag="VF240_13140"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEX7176200.1"
                     /translation="MPRISHGKVVTLAQVDAALKSIHLINASFGRQVINVAPLVLTRF
                     DYLFPDLQNDPQSRLPEHPDTVKNLRELGKSMSEAPGQQSDSNIPSAYTYFGQFVDHD
                     ITFEAVTRDIPLNDDNLAPLPLDEARQIKNIRTAILDLDSVYGSYCNDTDCQPPPRQG
                     EKMLLQMVAGTPLEGHDYFNDLPREFDPLLNGAAKIGDGRNDENVMVSQMHVAFLRAH
                     NAIVDLGNDFEAASRLLRQHYQWIVIGDFLDRIGHPETLAKVRADPKKFYDPPDDSFF
                     IPLEFTVAAFRFGHSMVRRTYDLNEEQTDTPFGFTLMSHALDDYHNLLGNWVIRWKNF
                     VRGGVNVARRLDTQLVEPLEFLRDENGMLVKFETRLAVRNLLRGYVLRMPTGQAVAQA
                     LGLPPEHRMSAADIEGVADEVSHKQAQVLADLGFSGRTPLWFYILAEAAKFGSGNSLG
                     PVGTTLVASVLIGLVRRSHDSILKEQDWAPTLGPTPGQFTLPDLLRLAGVLAE"
     gene            complement(36326..38863)
                     /locus_tag="VF240_13145"
     CDS             complement(36326..38863)
                     /locus_tag="VF240_13145"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX7176201.1"
                     /translation="MIKKLLLSVVALCAATQLTHARPVPSPSPEVASAAAPSDPPAKK
                     QDVKAGGAKAAIVLPIEKKQPVRIVKFDKAPAVDGKLDEEVWKTAAVFKDFYQTNPGD
                     NIAPSQPTEVFIGYDAKNLYLGVRATDEPDKVRATVAKRDNVFGEDNIRFFIDTYNDQ
                     RKAYILGFNPLGIQQDGIRTEGQNDDFSVDIVMESKGVIGETGWTLEVAIPFKSLRYV
                     AGKDKLWGLHIWRNIDRNNDEIDSWMPISRDRSGLLNQAGHLTGLEGISTERTLELIP
                     SLTVSETGKRIRSMTRSQSRLTGVADQGRFINQPIDLDPGLTAKWGITPTLTLDLALN
                     PDFAQVEADATVNTANQRFPIFFEEKRPFFLEGIEIFRTPIQAVHTRTIVDPDIAAKL
                     SGKHGKTTFGILYATDNAPGNYTEDQRYDPDNLPAAIDRLDGQNATVGILRLKRDVGK
                     ENHLGLLATAYNFADRSNYVGGFDGRFRFDKMTTAEFQVLGTNSRRCDFAAEAADDTC
                     RTLNGFAYAYNVTRSARHTWMNVNGSGRTSGYRADVGFTRRLNTNNHNLNFEYFTEPK
                     PKATIISWNTYHYFGGNFDWQGRLQNWTYEGQVGPNLQRQTYFRTGFNAGYERVFAYE
                     FGETAFASGRPEHSDYSKNFFAYMGSTPTKRISVNYFFGYRWGQLDYDFGAGPRFPRA
                     SQAAVRAREAEESGRCAEEEPAAVCGAPLDPGAGDFLYTNGGLTYKATDDLNMSVSFN
                     KNRLVRHDTGLVAFDTTIYSLRTTYQFTRFAFARARIDYNTLNSNARGQFLLGWTPNP
                     GTALYAGYNDDLNRGFVNPYSGDLEPGFRRNGRTFFIKMSYLFRRSL"
     gene            complement(39162..39638)
                     /locus_tag="VF240_13150"
     CDS             complement(39162..39638)
                     /locus_tag="VF240_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176202.1"
                     /translation="MRRSNTFELAAGLLLLVLLLAATAEGSPQKHKHREHKHKEKRFE
                     AVVRVDARDYAGRYIGIDDSYVLEVGTEADGRLKITSREGARRAELRDIRLDGARLSA
                     TKVYEDGSTAKFEGLFADRVLNGERRFGVLVSGLDLRLQGLTTLSRVFYTLFTEPR"
     gene            complement(39761..41557)
                     /locus_tag="VF240_13155"
     CDS             complement(39761..41557)
                     /locus_tag="VF240_13155"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7176203.1"
                     /translation="MRKTTATISLLAALAFTGAACARGHGAALSAESVEAATEQSPQT
                     ARQAPPFVNVEGADLAAKLEAASRQARTSSPRAPFWTAYTFDVRPGVAVDPGGGEFHG
                     SMNTMGGIHVFIGTTRAGTTAETRNLGLFVLRDPASGKLTRMEVYNLDRAREYSGYPV
                     YFAGRAGNEESLAYLRPLAEQSGDPRLQERATLAIALHDDRRVPDILKTFIRTSKSEN
                     VRSAAVYWLGHTGGEIEFLAALVRGNQEKTDLRRTAAHAIGASRDREALSTLQTLYNA
                     ALPEDVKRGILHAVNDNENREEAYAFVIKVAKSDTDSDARRTAVHIIGESERDGAVDD
                     LMSIFANDRSEDVKRTVIHALAEHRSPRAEAKLLELARTAEPPDVRKQAIHQLGERNT
                     EAMVDELMRLYSQEQNVDVKRAILHAFSEMESPRAQSRIVEIARDQSQPGDVRRHAIH
                     QLGERGESALDELVRIFDADRNEDVRRQILHSLSEMDSQRAEDKLFEVARRADNSDLR
                     RQAIHWIGEKASKRSLDFLKDTVNASDADTEVQKQAVHAISEKPPEQAVPLLIQIAKT
                     HRNAEVRKAAIHWLGESGDPRALEYFREVLSK"
     gene            complement(41795..43540)
                     /locus_tag="VF240_13160"
     CDS             complement(41795..43540)
                     /locus_tag="VF240_13160"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7176204.1"
                     /translation="MSYALNALKQVRPNARALLVAVAALGLSATGAGSAATKADGTDQ
                     DLKKFNQFVQTSNTPAMRALREGRDLIDAEQWAKAAEKFSQFVNAYPKDRDVDVALYW
                     LAYSLNKQGDPRGAAAQLQRLLKKHAQSSWADDARAMLNEIAGTLGEGGIAQNTLNDS
                     QEQEEIKIVALQSLFQSNPERAMTYVREIIKPGSTAGPRLMEAAVSLVGAHGGAQAIP
                     FLLDIARSQADEKLRARAIHSLANEGGEAVLDPLIQLYDAERNSDVKRQMLHAFFEMD
                     EVPRAHAKLLAIARSRTESDDLRRAAIHWIGERETPAAFDELVQLYNSEPSIDIKRQI
                     IHGFFDTKDARAHSKLVEIARSPGEHIDMRRQAVHWLGEQKSPAAFDSLAQIYGADSN
                     VDIKRQIIHALSEMDDPRAQAKISEIARSASESVEVRRQAIHQLGERGESALDELMRI
                     YASERNNEIRRQIIHAFSEMKSPRARQKLIEAARTGDDVELRRAAIHQLAEGDDAQTL
                     DMLISMYDAETQHEVKRQLLHAFSESKHKRALQKLMDVARRDSSVELRKQAIHWIGES
                     DDPDALKFLEDLLKP"
     gene            complement(43567..44733)
                     /locus_tag="VF240_13165"
     CDS             complement(43567..44733)
                     /locus_tag="VF240_13165"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7176205.1"
                     /translation="MNCDWAEKISLLIDGELPPQERRATEAHLESCAGCRRTKEDFLS
                     LRSVVNSYPLAADATDERRALEKILASAAHSTRDASDAAATTVPRTSWRELLVWPRLT
                     PALGAALAVLLLAVALGVVWSVSRRDAGQRLVAEGTGQDKARRSSNGPTQSDAPAPKA
                     SNPDASTPDAPASNAPAPGGPSEPAPKKDAPGGVNAPGRKLFNVPRAIERREDIVARH
                     TTRTATPAGGATLESASSAAAARSESAADALAEFKPLFDVADGHAGDESALSVDAEPR
                     GDTARHLEQAQILMRTIRNARPGDRPGAIADERRRSQQLLYRNIVLRREAARAGNAPV
                     EHALDSLEPLLVDIANLPERASREDVSVIQERMRRKNIVAVLQANIAAVPRTYQ"
     gene            complement(44730..45308)
                     /locus_tag="VF240_13170"
     CDS             complement(44730..45308)
                     /locus_tag="VF240_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7176206.1"
                     /translation="MARDTEEFESFFHETYPSLCRFLECMLAGGAARASAQDIAQESF
                     LRLHNEGLERFPAGEARFWLYRVARNLALNELSRAGTRQRLFGRVVEALGRRGHDPEQ
                     ELEMNERRKIVLEMLKQLPEHQRAALLLREQEEMSYREIADVLGVTEGKVKVDVFRAR
                     SSLRERWARAQKTLAAGAAGEASLSGLPKLIL"
     gene            45775..46164
                     /locus_tag="VF240_13175"
     CDS             45775..46164
                     /locus_tag="VF240_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7176207.1"
                     /translation="MLVVDDYEDTRVLIRRQLELGGFRVVEASDGREAVERAASHRPA
                     VILMDLNMPVLDGFTAALRIREREETRGVPIVALTAYDTAESRAAARAVGCSEYVAKP
                     VAFDALLLLVARLTQGSRVEAPGAEPV"
     gene            46369..>47276
                     /locus_tag="VF240_13180"
     CDS             46369..>47276
                     /locus_tag="VF240_13180"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176208.1"
                     /translation="MRLLLLTLTVLLACAASSRGSGRDDSSAPPEPGVGRDLARSRAT
                     HYRGVRYALNLTLRLGAEFLDGDIRISLTLDNPSEDLVLDWRVLKKDAEARGRVSEIT
                     ANERAVADARFVNDHIVIPSAYLRGGDNVLRLKFRSPVSASGSAVTRYIDREDKSEYV
                     YTLFVPSDASTAFPCFDQPDLKARFTLRLTTPESWRVVSNTDSHVQPEFDETSGGHPQ
                     SPPAGMLWHWFAETEPLSTYQFAFAAGPFVEFKEGGGDGPGKQTATPQPSVLKKGRRS
                     SLKAVSDGNASKASGGDAFGMRVFVR"
BASE COUNT         7663 a        15202 c        15693 g         8718 t
ORIGIN      
        1 gcgtactcgc gccccatgtc gtgcgccgtc ctgaccttcg agccgaacgg cggccggcgc
       61 agcacgaact cctgcgcccc ggcgcgcacc agataagtga ggttcgagta gccggccgga
      121 aactgttcga ccacgagcgg cccctcgacg ccgggcagac gagcgcgcag gtatgcgagc
      181 agcgcgtcag tgttcagttg ttcgcccgcg cgcacggccc gtggtttgtc gagtagctct
      241 tcttcagcgt ccaccttcgc ctcctcggtc gccctcttca gacgcttatg tcaacgccgt
      301 acttcctgag cacgcttcgg gcgaccaccg acttgtgtac ctcatcgggg ccgtcgtaga
      361 tgcgcgccgc gcgctcgtgc ctgtaccagt aggcgagcac ggtgtcgtcc gtcaggccca
      421 gcgccccgtg cgtttgcacc gcgcggtcga ggacgcggtg catcacgccg gcgacgaaaa
      481 acttgatgag cgagatatct tcgcgcgcgg cggcggcgcc ctcgcggtcg atgcgctcgg
      541 ccgtgtgtag aactaacaga cgcgcggcct tgatctcggc ccggctctcg gcgatccact
      601 cctgcacgct ctgccggctc ccgagcggca ctcccggcgc cagctccctg cgtgcggcgc
      661 gctcgcacat catctcgaac gcgcgctcgc acacgcccac ccagcgcatg cagtgctgga
      721 tgcggcccgg ccccagacgc tcctgcgcga tgcggaagcc ttcgccctcg cgacccagaa
      781 ggttgcgtcg gggcacgcgg cagtcctcgt agcgcacctc ggcgtggctc gcgtagtcgc
      841 tgccctcttc gcccatcacc gagacgttgc ggacgatgcg gaagcccggc gtgtccgtgg
      901 ggacgacgat ctggctcgcg cgtttgtagg ggttcgcttc gtccgcgttc gtcacggcca
      961 tgacgatggc gaaggcggcg ccctcggccc cggaggtgaa ccacttgtga ccgttgatga
     1021 cgtaatcatc gccgtcgcgc gcggccgtcg tgctcatcca gacggggttc gagccggcgt
     1081 gctccggctc ggtcatcgaa aagcagctcc gcacgtctcc gcgcgcgagc ggcgcgagaa
     1141 acgtctcctt ctgctcgggc gtaccgtgcg acatcaacac ttccatgttg ccggcgtcgg
     1201 gcgcctgaca gttgaacagg tagtggccga gcggcgtgcg gcccagctcc tcgctcacgc
     1261 gcgcgaactc catgagcgtg aggccgagtc cgccgtgctc cgacgggatt tgcggcgccc
     1321 acaagcgcaa ctctttgacc ttctgccgca gcgccgcgag tcgcgggagc agctcgcgga
     1381 acgggcggcg cgcgaagtcc tgttccagcg ggatggcctc agcgatgata aattcgcgaa
     1441 tttgtgacag cagcgattgc agtctgtctt ctggatggct catggcgcgg aatgataaca
     1501 cctgagtgtg gggcaggcgg agttcaccgc agaggcgcgg aggacgcaga gggggcgcag
     1561 agggttgagt tgattcaact acgtatgaaa gggtcaagcc tctgcgcgaa ctctgccacc
     1621 tccgcgcctt tgcggtgagt ctcacccacc ccacactcaa cttcgagcag cgggagcccg
     1681 aatcacaacc atcttgtcag agtcggcgta gacgggttac gctggcctcc ctctgaggag
     1741 aagacgaacc aacatgcgaa tcaaccatcc cgacccgagg acgcgactgc tcgcggcgct
     1801 cctcgcgctg gcctgtctcg cggcgtgcgc cccggcgagc gcgcaggagg agacggccgc
     1861 gcgcgcgctc cacgccgagt ggcagcggca gcgcgtcgcc gacctcgacc ccgcgctcgt
     1921 caaacacgcc gacctgcaaa aatatctcgc cactctgcgc gcgcgcggcg tccccgtcac
     1981 ggaagtcggc cgcagtctcg cgggccgcga gatttaccag ttggagttcg ggcgcggccc
     2041 cctgaaggtc ttcatgtggt cgcagatgca cggcgacgag ccgacggcca cgagcgccct
     2101 cgtcgatctc ttcgcctatc tccacgccaa ccgtcagctc ccctgggtca aagcggtgga
     2161 agagaaaatc actttgcgcg ccgtgccgat gctcaacccg gacggcgccg aactgttcac
     2221 gcgccgcaac ctccagtcaa tcgacatcaa ccgcgacgcc cgcacgctca ccactcccga
     2281 gggccgcctc ctcaaaaaac tccgcgacga ctggcagccc gagatcggct tcaacctcca
     2341 caaccagaac tcgcgcacgg ccgtcggcga cactctcaag caagcggccg tctcactgct
     2401 cgccgtcgtg tctgatgctg cgggcacgga cactccgggc cgcgtccgca acaagcgcct
     2461 gtgcgccgtg atggtcgcct cgctcgcgcc cttcatcgag ggccacgtcg gccgctacga
     2521 cgacgagttc aacccgcgcg ccttcggcga catgattagc cagtggggca cgcccgtcat
     2581 tctcatcgaa accggcgcgc ttcgcgggcg cacgaatcaa gacctcgtcg gcctcaactt
     2641 cgtcgccata gcctccgccc tgcgcgcgct cgccgacggt agctacgagc gcgccgaccc
     2701 caaagtctac gatgcgctgc cgatgaacgg cggcggcatc tacgacctcg tcgtccgcgg
     2761 cgcacacgtc gtcaaccgcg tgcgcgaagg caagaccaca acggcgccgc cattcgcgac
     2821 agacctcgcc gtcaacatcg acgcgggccg cgacggccga gcgcgccgcg cctccgtgca
     2881 ggacatcggc gacctctccg tcttccgcgg cctctcggaa gtggacgcgc gcgaatacta
     2941 cgtgaccgtc cccacgggcg gcatgctccg caacgggggc gaggccacgc tgctcttcta
     3001 cccgaagaca caagcggcgc gtctcgactg ggacgcgccc gacttcgaaa ccaagttccc
     3061 gcccgaggcc gtcttccgcg gcggcgagtg ggtgaagcgc ggaaggcttc cgatcaaatg
     3121 aggcgcgcac acacgcggac gccgctcgcg gtcttcgtcc tcctcgcgtc gcttctgctt
     3181 cagggcgcgg cgcgcggccg tgtagtcacc ccgccgcagg ggtcgtcgcc cgccgaggtc
     3241 gtcgagcgcg gaaagtttcg catctacaag ctgcaatacc cggtgggcga ggagagttac
     3301 gagatcgcgc gcgagggcgg cgcgcacgtt ctgagcgcga agttcgagat caactatctc
     3361 ggcgacaagg tgccgctcac ggccactcta cgcacgcgct ccgcagacct cgcgccgctg
     3421 cgcttcgaga tcaaggggcg gacttcgacg cgctccgagg cgaacgccgc ggttgaagtc
     3481 ggcgagggcg gagcggcgac ggtgcgcgaa ggcacaaaaa cacgcacgga gcaggcggcc
     3541 ggcgcgcgca tcttcccgtc gggcggtctc cttcccgtct ctcttcagca ggcgctcttt
     3601 cagtacgcgg cgcgtcacgg cgcgttgcgg aatctcagaa cgctgccggt cgggcgcgtg
     3661 agcttcgagc cgcgcgggga cgaacaggtg gaggcgggcg gacggcgcgt gggcctcacg
     3721 cgctacagcg tgtcggggct ggtgtggggg cgcgagaccg cgtggttcga cgaggggcgg
     3781 cgcctcatcg cgctgataag tcctgacgcc gaactcgacc gcctcgaagc cgtgcgcgag
     3841 ggctacgaac acctcctgcc cctcttcgtc gcgcgcgcgg cggccgactc ggtggccgac
     3901 ctgcggaaga tttccgacgc cgtccgcccc ctgcgcgccg ggcgctacgc catcgtcggc
     3961 gcgaccttga ttgacggtac aggcacgccg cccgtcgccg acgcggtcgt gctcgtcgag
     4021 aacggacgca tcaccgccgc ggggccgcga gcgtcggtca agattccgaa gggcgtgaac
     4081 gtcttcgatg cgcgcggcaa atacctgttg ccgggcttgt gggacatgca cgcgcacgcc
     4141 gagcagggcg agtggatacc ggcgtcgctg gccgccggca tcacgacgat gcgcgacgcg
     4201 ggcaacgagc cggagttcat cctgcccctg cgcgacgcgc tgcgcgcagg gaaggtcgcg
     4261 gggccgcgtc tgctgctcgc cggcatcatc gacagcccgc ccggcgcgct cggcaacaac
     4321 gtcgccgaga cgcccgaagc tgcgcgcgca atcgtcgggc gctacaagcg cgacggctac
     4381 gagcaggtca aaatctatca gtcgctcaag cccgaactcg tctcggtggt cgcggccgag
     4441 gcgcaccgtc tggggatgag tgtgaccggg cacgtgccga cgggcgtgga cgcctacgcg
     4501 gccgtcgagg ccgggatgga tcagatcaac cacgtcggct tcatcttgcg cgcgatgcgc
     4561 gggcgcggct ggcgacccac gccgggcacg cccccgccgc ccctcaacct cgactcgccc
     4621 gaggccgccg ccgccatcaa gttcttcaaa gagcgcggca cggtcatcga cccgacgctt
     4681 gcgcgcggcg agttgaacgg acacgcgctc gacatgccgt tcgtcaattt cgagccgggc
     4741 atggcgaaga ccccggagga gctggcgtcg attctcaaca acacggggct gcccccagaa
     4801 gttgcgccgc gcgccgccgc gtcggccgcg ctcgcggcgc ggctcaccgt gatgctcatg
     4861 cgcgcgggcg tgcccgtcgt cgccggcaca gacttggtcg tccccggcca caccatctac
     4921 cgcgagctgg agctgtatgt gcgcgccggg atgacgcccg ccgaggcgct tcaaaccgcg
     4981 accatcattc cggcgcgcgt gatgaggctg gacaaagagc ttgggacaat cgagcgcggg
     5041 aagtttgccg acatgattct cgtggacgcc gacccgctcg ccgacatcag caacctgcgg
     5101 cgtgtgcgct acgtcgtcac cggcgggcgt ctctacgaga gcgcgccgct atggcggagc
     5161 gtcggcttcc ggccgtgacg ctcaagcttc ggagggaaca cggatgagac ggatgagaac
     5221 ggatcggcac ggatcaatcc gtgtgaatcc gtcttcatcc gtcccagccg tgttccccgc
     5281 gccgtgttcg tctttcgtga tgaaggtgtg ttcagcgcgg ggagagttgg tcgcgcaggg
     5341 cggcgaggcg gtcggggtag tcggtgatga tgccgtcaac gccgatgtcg agcaggcggc
     5401 gcatgtcgcc ggcgtcgttg atcgtccaga cgtgtacctg taggttgagg cggtgcgcgg
     5461 tctcgacgaa ctcccgcgtc acgacgcgcc agccgcgcag gtgttcgggc acctgaagcg
     5521 cctgcatggc ggggcggtag gtgtcgccga ggcccgcctt gaagagggcg aggaacttgt
     5581 agccctcgga gagagtggcg gaagtggcga cctccgggca ggcgcgtcgg aagtcgtcga
     5641 gggtcgcctg tcggaacgcg gcgacgagca cgcgcttctc cacggcgtgc tcgcgcaaga
     5701 gccggcagag cggcggggcc agcgccggct cgtcctgctt gatctcgatg acgaaacgcg
     5761 ccgacgcgaa acgggcgagc acttcttcga gggtcgggac gccgtgtccc gcgccgcgca
     5821 gggggaaagt ctccccgccg tcgggcgtcc agcggtgggc ggcgtcgagg cgcttaactt
     5881 cggcgagcgt gagttcactg acgcggcccc ggccgtcggt cgtccgctca accgtctcgt
     5941 cgtggtggac gactagtgcg ccgtcggccg ttgagcgcac gtccatctcc aaaacgtcgg
     6001 cgccggcggc gcgcgcgcgc tcgaaggcgt ggagcgtgtt ctcgggccag aggccggcgc
     6061 cgccgcggtg cgcgatgatg agggagcggc gcacgccgcc gaagtattcg tgttcggcca
     6121 cgggcctccc ttcccgtagc gccagagcga cgtacaggcc ggcgacgacg gccgcgacga
     6181 gcgccgcggc cgcgagccat ctggcgcgct tcgtttttaa actgattcgc atgatgcgcg
     6241 cgggcgcgcc ccggcggagg gactaattaa caggatgagc aggatgagca ggatgagcag
     6301 gagatgattt gaaacttgaa atttgagatt tgaaatttga aatttcaaat ctacttatcc
     6361 tgcccgtcct gttaatcctg tcaattaatg tttgaattgc ttttcggctc actccaaatc
     6421 ttcccgcgtc acgccttcaa tctccatgat gaaatccgcg ccgtaggccc gtgacggcgt
     6481 ctgaaagccg gcgggcgctt gccctgcgcg cacgcgctcg acggcggcga gcgccgtcat
     6541 ggctgtcagc gtgtagccct cggggccttg gaggcgtgag acgcaacgtt tgccggcggc
     6601 gtcctcggcc tcgccccaga cgatgctttt gccgcgggcg cgctccgcgt cgctcggccc
     6661 cggcgggcct gacttgatgc gcgacttcat gtagcgctgc gcccacggcg aggcgagcag
     6721 cgcgccgacg tgccggctca cgaccatcgc ccagcgcatc gacggaggca tcgccgcgta
     6781 gacctcaacg tccgcaatcc ctgtgctgtg gaaggcggtg gacacgtcgc cccaggggat
     6841 ggtcgtggcc ggctccggcc ctttgccgaa gtctatctcg cgtgtgtgcc acgcggcggg
     6901 cacgggcgtc agcttgccgc cgcgtcggat gaggccgccc cggtcgatgt tctcggtcat
     6961 cgtcgtcgcc gtgccgcgcg acagacggcc gctgcccttg atggccagcg tcagatgcac
     7021 ggccgccggc aggcgacgct tgaggtgcgc ggccagacag tccgacggca ccacgtcgaa
     7081 gccgacgccg ggcaggagca tcacgccggc ctctcgcgcc tcggcgtcgc gcgcggccaa
     7141 gccttcgaag accgtgatct cgcccgtgat gtcgaggtag tgcgtgcgcg tccgcaggca
     7201 ggcgtcggcc atcggcttcg aggtgcgcga gaaaggcccg gcgcagtgca ggacaaccgc
     7261 ggcttccttg agggcggagt ctacggccgc cgcgtcggag aggtcgaaga cacggtgttc
     7321 gagctttagc tcgctggcca accggccgac ctcttcggcg gagcggcccg cgagcgtggg
     7381 gcgcaagccc cgctcagcgg cgaggcgcgc gacgagggcg ccggtgtagc cgttggcgcc
     7441 gtagatgatg aagtctgtct gcataattta gtaaatcgtg aatcgtcaat cgtgaatcgt
     7501 ggttgaagcg gcggcagcct tgaacagtga atcgaaagtc gaatcatgga caatcgattg
     7561 acgacttacg atttaccatt cacggctttg aagtatacat cagggcggcg ctgtttttag
     7621 ttctggccgg cggaaagaaa ctcgattaaa atgccgccgc gtcaactgct gaaaaagtgt
     7681 tcttccgtaa cgtcgtcccg cttccgcgtt tttcaaccga ggagaaagat catgcgtaga
     7741 gtcatgctgg ctgtggccgg gctgttgctg ctcgcgcccg cgcccgacgc ccgcgcgcag
     7801 acggccgacg tgatcaaaga cttcgacgcg tgggcgctca gggagatgtc cggcctcaac
     7861 gcggccctcg cggcgaagca gttgcagccc gtgcccatgc tcacgcgcga ggagtgggag
     7921 aagacggacg ggcaaataaa aggatgaggg atgaaaggga gtaagcagta agcagtgagc
     7981 agtgagcagt cagaacgctc aaccggcatt gactgctgac tgcttactgc tcactgcttc
     8041 ctatcttctt tcatccctca tccctccgcc ctcatccctt ccagataagg ggcggcgcta
     8101 atcacgacga ctggaggcga cgaatgttta aagagttccg agagttcatc aaccgcggca
     8161 acgtgatgga tttggccgtc gccgtgatca tcgcggcggc cttcggcaag atcatcacca
     8221 cgctcgtcga gggcgtcatc atgccgccca tcggattagt cctcggccgg gtcgatttcg
     8281 cgagcctctt ctacacactc gacgcgtcga agggcgtccc cgtctcgctc gccgacgcga
     8341 aggccagagg cgtccccgtc atcgcctacg gacagttcct caacgacgtg gtgggcttcc
     8401 tcatcgtcgc cttcgtcgtc ttcctcatcg tcaaacaggt caaccgcctc aagagcaagc
     8461 aggacaccga cgaaggcccg acgacgaagg actgccccca ctgtctctcg accatcccgc
     8521 tcaaggccac gcgctgcaaa gactgcacgg ccgacctgcg cgccgcctga aggctcggac
     8581 gccgtggcgg gtggcgcggg cggcgaaaaa tgttcttgtg agtttgtgag ccggggtgtt
     8641 agattgcgtc cgttgccgcg gtagcgcctt cgccccccgt tcgttctacc gtccagcttc
     8701 tcgatgaaac tattcggact agcaatcaca cgcccgttct ccatcctgct ttcgctgctc
     8761 tgtctcctgt ctcaggccga gggtcttcgc tcacaaacga ggagcggggt ggtgcgcgcc
     8821 ggagcaggcc gcgcatccgt cgaaatcctc gacagcgacc gcggcaagga cgggctgctc
     8881 ggccccgtgc gtcgcgtccg cacggaagtc gcccgcctgg acacgggagt gacggcgtct
     8941 gccgaaggcc cacgctcgct tctggagatc accgtctacg acgttggcgg caagagaatc
     9001 gccaaccgga cccacccggt cgccgtcgcc ggcgccggcg gcggcggcga gacatacgag
     9061 tacgacgagc gcgggcaact acaggcgacc gtcgtcaagg atgaagccgg cgccttcctg
     9121 tcgcgcacag tctactctta tgagtacgac acggcgggca actgggtgaa gatgacggcc
     9181 tcggtcgccg tctccgaggc cggcgcggag atgctcgaac ccgtcgaggt gacctatcgc
     9241 gtcatcactt actactctcg cggcgcgggt aaggccccgg ccaatgctgt cgccgcgccg
     9301 gcgtccgccg gggacagccg agcggtcaac gccgaaggcg cggctgaggg cctgcgcccg
     9361 acaacctccc ctcgttcttc aaaggctgca agcgaccggc acgacggcgc ggacggcacg
     9421 gacggcgcgt ccgtgccgtc cgcgccgtcg gtcgaagtaa aggccgagcc acccgccccg
     9481 atgttcgacg ccggcttgat caacgacaag gcgctcagcc tgccgaggcc tgcctatctt
     9541 gtgggaagca gcgcgcagcc cagccccgtg gtcgtgaagg tcgaggtcgt cgttgacgag
     9601 accgggcgcg tgatttcggc gcgcggcacg gaggggccgc gcgtgctttg gtgggcggcc
     9661 gagagcgccg cgcggcaagc gggtttcttc ccgttccgcg agggcgggcg gcccgtcagg
     9721 gcgcggggcc tgctcgaata cactttcccc ttcgagccgc gctaagggcg gtggcggccg
     9781 aggcaacacc gcaggacaaa acgaacgcct acgggccgcg gtctgcgacg ggagcacgac
     9841 agacaccacg gatgcgcgaa gcacgcttca tggtcctgac cggacacctc gatgaacaac
     9901 ccctgccggg tctcatcagg acgctccacg cccagcacaa gacgggtcgc cttcaggtgg
     9961 attacgccga gtcgccggcg gccttccatt tcgagcaagg ctcactggtt gacgcgcgtc
    10021 tcggcgcgct gcgcggcctc gaggccttga tgctggcgct ctcgttgccc ggcgccccgt
    10081 tcaacttcaa cccgctcgtc aagccgccgg agaggacggt cgaggagcgc gagcgccaat
    10141 ttatccgcga gctgctcgac gccgccccgg ccggcactgc gctcgacgtg ccggcgtcat
    10201 cggcgttacc cgccgaggcc gagacgacga cgccgcgcgt cgtccccctg cgcggggccg
    10261 ccctgccccc ggccgcgggc gaacgactcg ccctgcccgc gcccgcggac gaaagtctgg
    10321 tctacgaagt aaacgcggcc ctcgcggcgc actcgcgccg ctttagccgc gagcgcgcgg
    10381 cctacgccgc cgtcatcgcc ttgctcctgc tgctcacatt cctctcgcgc ctgcgcaacc
    10441 cggtcgcccc ggcggcccct ccatcaccgc cgcaggctgt gcaacagacc gcgccggacg
    10501 ccgcctcgcc gccggcagac gaacggcttc gccccgccga cagttcgggc ggcgctgacc
    10561 cggccgacgt gccggcgacg gcgcggcgcg acgagccggc gcgcgtcgag cgcccgtcgc
    10621 gtgagacgcg gcagtcttcg cccgccggcg ctgcctcttc gaggggtgcg gcgccgctgc
    10681 cggtcgggca gacgcagacg agcgcggagg ccggggtcgg cggcgcgggc ggcgaaccgc
    10741 cggcggggcg cgaacgggcg gccaatgcgc gaggggaaag ctctgtacgc atccttctgc
    10801 gcgtcgagcg cgggcgcgtg ctccaggccg tcgtccagaa ctcgcgcccc ggcatggagt
    10861 cctacgaggc gctcgcgctg aggatggcac ggcagcggcg ctatcccgca gacttcaacg
    10921 ggcaggacac gctgcaactc aaggtgcggc aataatgcct ctccccactc gaaggcgaag
    10981 atgctcctcg tcgaacgcaa aacactgagc ctcacggtcg atgagccagc ccgtctcgaa
    11041 ctgctcgacg cgctccgcgc cgctcagttc gcgccgctca tccgcacggc gcggcggcgc
    11101 tccttcgagc tgtcgctggc cgcggcgggc ggcctccccc cggcccccga attgatcgac
    11161 gacttcgaac gacaggctct ctcgtcccgc ctccgcgaac gggccggccc ctacacgccg
    11221 ggcgacctct tctgctctga agacgcaatc ttctatctcg ccttcggcga cgacgccgcg
    11281 cgcggtcacg aggccgtcgt cgccggcgtc gtctacgata gggagtcggt cgatcccctg
    11341 cgccggttcg agttgttctg ccagagggtg cgcgagtcga tgcgcttggc cggggagggc
    11401 gaagcggagg cggaggccgg ggagttagtg gcgccgccgt gggagtcgca cgacgtgcgc
    11461 cggttcccgc ggggactgac gcgctacgtc gcgcggcagg atgaggaggc cgcccgcgcg
    11521 atgatgttca gggagggctc gcgcgagcgt ctgcgcgccg ccgaactcct cgccgacgaa
    11581 cgggtgcgcg ccttcctccg gcgcgagcag gaggcgcgcg tcgagggcta cgcgccgcgc
    11641 cttttgtccg gcgaaagccc atcgcccgag gggcttgtgc ccgttgaaga actcgtcgag
    11701 gccggactga tgcgccgcga ggtgcgcgtc acgtgccgaa agaccaggca cccgctcttc
    11761 gacctgccga cgccggacgc gctcgcgctc gtcaccctga gccgcgcccg ttgcagcctg
    11821 tgcgcgacgg aggtggccga cgaggtcgtc gaggaggtct acaatccgac ccgcctcgcg
    11881 gcggcgcttt tggccgacgg ctcgtggctg cgtaaccgcg tctaccagat cgtccgcgac
    11941 ctcggcgtgc ccgagagcga gatcgccgcc ggcccccccg ccgagggcgg cgagtcgcac
    12001 cttgtcgcca acgtctgcgg cgacgccttc ctgttcgccc tcagggatgg agagttgtcg
    12061 ccctccttca gccgccgcgt ggtcgaggcg gccggcgaga tcgaggcgcg ccatgtcatc
    12121 gtcatcgtca ccggcgcggc ggagggtgag gggcggatgc gcctctacga gtatgcctgg
    12181 cgccgcgccc gcgagggccg cgacgtcgat gtcgccgtcg gcgagggcgc ggccggcgcg
    12241 cggtcgtgta tcgagggcgt cttcgagcgc gcggtcgggg gcaggctcgc cagagacctg
    12301 tgcgcgctcg acgacgccct ggggatgaac gcgagccgcc tcgtgctcac caagttcaaa
    12361 atgaggaggg agcaggcggg cgccagacgc gcggagacgc gccgcgccgc ccgcccggcc
    12421 ccaacgcttc cgggacaaga cggcgaggaa tcagacgcac cgcccgcgcg tctgcgtttg
    12481 agttcgtgag accgaggtcg ttaagagtgt tacctgccgg ccgcggcttg ctgcgactct
    12541 tcgcggatga tggcggcgaa gtccgtgaga aagcgaaaga gcggcttgcc cgtcgagcgt
    12601 ggggcgctgg tgttgacttg gacggcgacg gccgccttga ggtcggggaa gtaggccatc
    12661 tcggtgaggt agccggggaa gaagccgctg tggccgtaga ccacaccgag ttcggtctcg
    12721 cggatgatga cgccgagtcc atactttgtg ccggggccga gcttggccgc gacgccgtcg
    12781 agcacctgcg gcatcagcgc gcggtcgaag gcgcgcccct cgtagagcgc acgcgcccag
    12841 cgcgcgaggt cttccgtggt cgaggcgatg ccgccgcccg tccactcgaa ctgcgggttg
    12901 atggcaaagc ggccagccga gtcgatcatc gcgtccgtgc cgccgaaggg gttgtcggcc
    12961 ccggcgtagc cttgcgcgag gccggggatg cgccgggcgt cggaaggaac ggtgcgacgg
    13021 agtttgagcg ggccgagcag ccggcgtcgt acttcatcgt aataacgccg gcccgtgacg
    13081 cgttcgacga tcatgccgag cacgatgtag ttggtgtccg agtagtccca ccctcggcct
    13141 gcggcaaacg gcggcgcggt gtcgaggacg taggcgacta actcctcggg cttccagacc
    13201 ttgtcggggt tcgcggtcaa gtccttcgtg aactgctcct tgaactcata gcgcaccagc
    13261 ccgctcgtgt ggttcatgag catgcggacg gtgatgtcgc gcgcgttcgg aagccgcgcg
    13321 aaccacgact cgcgaccgag atacttttct accttatcgt ctaaaccgat cttcttctcc
    13381 gcgacgagtt gcagggcgac ggccgccgcg taggtcttcc cgacgctgcc ctgaagcatc
    13441 aaatcgttgg gcctcatcgg cgtcttcttc tgtgtgtccg aagtgccgac ggagaggccc
    13501 agcgaggtgc cgtcggcgaa ggcgaagccg gcggtcgcgc cgggaaagcg gcccgccgcg
    13561 tgcagcgcgt cgagcttctc ctgaagccgg cggcgcaggc gcgcggccgc gtcggcggac
    13621 gacacgcgcg gcgtcggctt ttgcgtttgc gggcgggccg ccgcggcggc gagtgcgccg
    13681 cagtcgaaca ggagggcggc cagcagtaga agtctgagag tcatcgcggg ttgcaatcgt
    13741 tcgcctcctc gaaaggtttc gcgcttcaat ggaaaacaaa accagcgcgc ctgatattaa
    13801 caccggcgcg cggtcattaa atccgttatg tgattaagcg gtacgaaatc ggggcgggaa
    13861 ggacggaggg aaggcaaaag taaaaaggca aaaggcaaaa gtgaggaagc gcctcgcctc
    13921 tgcactttgc cttttgcctt tttacttttg ccttcttcag cggacgacgc ggaaggtgta
    13981 gcggctcacg tcttccagac ggccgccggc cgcacggccg ctgaccgtga catgataatc
    14041 gcccggcccg acgacgcttg cgggaatgtc catgtcgatg gcggcggagc cggccgccgg
    14101 tcgcagctcg tcggcggccc agacctcggc gcgctctgcg gtcagcagcg cggcgcggta
    14161 gctttcgtgc tcggcggcgg gcagctccag acgcaggcga agggcgtcgg cgccggcggg
    14221 gagttggatt cgcgccggct cgccggagcc gcgtgtgagg ccgggcgtga gagtcacggc
    14281 gtagggatta cggaacgcaa cctcgcggcg ggccggacgg tcgaacgcca cccaggagac
    14341 ggcgacgacg gcgagcagca gcgcggcggc gagggagaag gcgagcgcgg ggccacgcgc
    14401 gaagaggagt tggaaaaggt tctgcttgcc gggggtcgcg ggccgagtcc ccaagtcgcc
    14461 gggagtttgt tctttggtcg cggccggctc ttcgcgtaga ccggcgggca aggaggcgag
    14521 ggcgacggcg ctgccgggag cgtccgtctc ggcggcgacg tagcggcgca gggcgcgcgc
    14581 gaagcgcagc tttttgtgtc gctcgggcgc gagcaggaag tgtgtctcga agtcctgatg
    14641 gtcggccgcc gcgagttcgc ccgacaggta tcggtcgatg aggtcgtctt cggcgatgga
    14701 aagctcgtcg tggaaggcgg cgtcggtcag caggcgttct tcgagcggcg tgcgctgctc
    14761 gggcgctagt tgcccgagca ggtattgtct gagcgcgccc atcgcttccg acgttagttc
    14821 cacctattgc gcgcccctcc cgaaagcgcc ccctgattgt gatgtctttt tgctcatatc
    14881 cgtttcaccc ggccgccggc cgcgtgagac actcctcgac acacttctgg aggcccagac
    14941 ggatgcggaa ggcgcgtatc ctcaacgcgt tgagcgcgat gcccagtcgc tcggccaaat
    15001 gtttgcggtt atcgatcttc gcccgctttt cctcgcgata gtactgcaag accagctcgc
    15061 ggttgtggga cgaaaggccg ccgacgcagt tcaggaggca ttcatacacg cgctcgtcct
    15121 cctcttccgc ggctggcgcg gtgacggggt gcggcgcggg cggggcgggc ctcctgcgta
    15181 aatattccag gtgtacgttg ttggccacac cgtagaaata gagggcacgg tcgcccgtgt
    15241 agtcggcctc tatctggggg agcttggcgg ccacccggtt gataacttcg tcggcgaggt
    15301 cttccggctc gtggcagccg cggcaggtga agattttgat gagggagcgg cgaatctcct
    15361 cgtacttccg gcccgcctct tcggggccgg cgccgagcca ggcgagcagg cggtcgaacg
    15421 cctcctgcgt gagagaccag tttttcttca tgggggcaaa ctcgcggctc ggcggcgact
    15481 actacgcgcg tgtgggtgtg tgcgcgtggc gaaagttctt caagctttca cctgtcgggc
    15541 gccggccgag ttccttcttc tccgtctcag ccggatcgcg ccgagcgcag cgaatgatat
    15601 cgcagccgcc ccgccgcccc aagccgccag cccccactgc gcgaagtagg cgggcccgtc
    15661 gcccatgccc ttcaactcgc cgtactgccc ctggatgacg aaggccgccc agtagtaggg
    15721 cgcgcgccaa cgcttctgcc gccacatggc gagctgcgcc tgacgcagcg ccgccggggg
    15781 cgcaaggccc tgctggaaca tcccagcgta gaagtgcttc atcaattcgg cggtggcctc
    15841 gtcgtccacc ttccacagag aggcgacgac cgcgcccgcg ccggcgtaca tgaagccgcg
    15901 cgtcagcccg atgagaccct cgccgcgcac ctcgcggccg agcgccgtgt tgcaggccga
    15961 gagcacgacg aggtcggcgc tcaggtcgag gttgtagatg tcctgaagac tgaggaagcc
    16021 gtcctgcggc tcgccccgct cgtcaacgag cgacagcacg atgccggaca tttcgggccg
    16081 ttcgctgtcg aggatgccgt gcgtggcgaa gtggatgacg cggtgacgcc cgaggcccgg
    16141 ctcgacggcc gcggcgcggc tggccccgaa gcctgtgacc aacgcgacct cgccccgtgg
    16201 gagcgccgcc gcgatggctt cggcctcctc gcgcgaggcc agcaggcgcg ggacgccttc
    16261 gccgccgaag agcgcgctca cgtctctgat ggcgcggtgc acctcctgct tgccctgatg
    16321 cgcgacggcg tccgccgatg cgtgtggcgg ggccgcatcg gggcgcgacg agtccgcgcc
    16381 gcggacgcgc gcgtcgtcct tgtcgaagac cgggtcggcg aagacggcaa ccgaacggcc
    16441 ggcctgctca tagcgggccg ccccgccttg cagcgtgggc agaatcgagg ccgacgggag
    16501 gctgacgact tcgtgtcgaa ggaacagggg aacgcgggcg ctgccttcgg ggcgggcttc
    16561 gggtcgctcc tgtgcgccgc ccgccgacgc ttccgtgccc ggctcggtca gcgcgccgaa
    16621 cgggatgtac tggagagcgc cctcggtcac caccaaaagc cttcggcccg tgaggtgctc
    16681 cgacgccggg ccgataatca tgtcgctcag acgctcggcc tcgcgcccgt agtccgcctc
    16741 cgcctgcgcg acgcgagcct gccgctcgcg ggcgctctcg ccggcaaccg gctgcggcgc
    16801 aaccagcaaa cttctcacgc ggttggccgc gtcttctatc tcggcccggc ccggaagttc
    16861 ataactatga atggtcccct tagtcaccgc ccagaggtag cttctctcct cgcccagcga
    16921 atactccagc agcagtgtcg agtcgtccac gacgcgctcc tgaatcgcct tcagtccgag
    16981 aggctgcgtc tgaagcgacg ccgtgtgctc aaggcttccc gccctgatct gcgcccccac
    17041 ctcgcgcagt tgcgcgctga gttcgtcgat ctctttggcg agcgccgacg cctcggcctg
    17101 ctcgccgccc gtctgaagcc gcgcccgccg ctgctccgcc ctgtcgttca actccttccg
    17161 cagcgcgcct tcccgctcca gcagcgcggg atcggccttg cggcgcagcc cgacgcgcgc
    17221 ggccgagagc gtctccagca gcgagcgcgc ccgcgcccgc tcgctcgcct cgaaggcggc
    17281 ggcggcgaac ccttcggacg ggcgctcgcg gtgcaggcgc atgagcaggt cgatgtaaaa
    17341 ctcgtactgc tcgcgcaccg aggcgaagta cgacgcgcgc aagtcctgac tgacgacctt
    17401 cccgcgcagc gattcgacga cccccagcgc ctcctccgcg cgcgcgctcg cttccgccag
    17461 acggccgctg tcgcgctcca tgcgcgcgat gttgtggagc gtcgcggcct cggcggcgcg
    17521 gtactccgcc ttccggctga gcggcagcgc cttcgcgtag tattcgagcg ccttctgcct
    17581 ctgcccccgt ccttcgtaaa cgcggccgat caggttgagc gtgtccgtct cgccccggag
    17641 gtcgttctct ctctgccaga atggtagtgt ccggctgtag tattcgagag ccttgtcttt
    17701 ccacccccag gaatcataaa ttcttcctat ctctctaagt tcgtacaact ccagtcgatg
    17761 attgcttgtg gctttggaaa ttgagagcgc ctgttgaaag cactcaagtg ccgcctgaga
    17821 atcacccaat gagtgataaa ccttgccggt ttcgtagagg gtggccgctt ctccgctgcg
    17881 gtaattgacg cttcggaaga gagataacgc ctggttgtaa tattcaagag acctcttctt
    17941 ctcgccaagc ccgctgtaga caaaggacat tccatttaga attcgagcct cttcaatcgg
    18001 gtcttttatc ggctgaatga tttgcttcgc actctcaaag aagtttaatg cttcctgact
    18061 ttcgccaagt cgtgagtaaa gtcgcccgat agcattaagt gtaagagctt gccctcggcg
    18121 gtcatgtgca gcttgccaga gaggaagaga ttgattgtag gagtcgaaag ccttccctat
    18181 ttggcccaag tccgaatagg tatagccgaa attcaaaagc gtcaaagctt gcccttttcg
    18241 atcattcaat tcacgccaga atggtagcgc ctgctgataa aattcaacgg actgctgcaa
    18301 cttacctaat ccgtagtgaa cttctccaaa gttgttaagc gcctgtgcct ctccgcggcg
    18361 gctacccgtg gctcgattca acttgagggc ctgagtgcaa agattgaggg ctttcggatt
    18421 gtctcccagc gtaaggtaga cgaagctgag ttcgttaagc gtctcgccct caccgcgccg
    18481 gtcttttatt tcccgttgaa gcgagagtgc ctgacgataa taattcagcg cattttggta
    18541 ctcgcctaac ggctggtaaa catcaccgat gcgcttgagt gtgagagctt ccgcccggcg
    18601 atcaccgacg gccttccaga aggctaacga atctttgaac ctgtcaatcg ccccgcggct
    18661 ggactgcgcc ttccactctt tcagcagtcg ctcaccgtcc gcgaaagtct tctcggcatt
    18721 gattcgctcc ctgtcctccg cggtggatgg tctgatttct tcaaccctca gctcgtaacg
    18781 tccgcgcgtc tgctgtcctt ccggcgagcg cacttccaga cggtagattc cagaggtttc
    18841 ggcgatgagt gaaacaggtg tcggcccgta atcacggcag tcgagttgag cgagcgcctg
    18901 ccctcccggt tcgtagagag tcacatccgc gtcgatgccc tgcggctcga cgaccgcgtg
    18961 gaggtattgg ccagtgtcga gcgtgatttg gtaagaatga acttcacctg cggagagttc
    19021 ttgtgcggtt gatttgccgg gtgcaggcag aagtgtactg accgcgccgt gttccgtgct
    19081 gcttgacttg gcgaaggcag tgagggacgg ggggcggggg cggctcttaa acgtcggctc
    19141 cgacagggtg aaagcgatgg ttaagagggc cagactatat ttgacatact tggtgaaaga
    19201 catccgtgca tctcgatgac aaccttctat tctctttcaa gctactatga ctccttcaaa
    19261 gcaagtctga aatagccaca ggaagattac cccagacttg ccgagcgttc cttgttcttc
    19321 ttatttgggc gatttcttcg gtgtcgtggt cttggcttgc cagcctaaga ctgccctatt
    19381 gacactacca gccttagatt ggcttccatt tctgtttgcc gagccaaatc taccctcaat
    19441 gctatgcctg ctgttctgca tcgttgcggc agacgtctta gaaattctac gcggaagtct
    19501 tttgatagac attttagcct cctttggttg aattcgggtc agatgagcga ggccggtaaa
    19561 actgtagcgc gcttcctacc tggcagaggg gttgcgtgtt gcgttcaagc gaatctcacc
    19621 tgagccgcaa cacttgatat tccagaatcg tcaccccgtc aatcaaattc ttcaacccct
    19681 gactgttgtg agggcgcatc atgccttatc gtccttgtcc ttgcgggccg gccctgtcgg
    19741 gtcggcgcgt tgtgctcacg aaagcgtcga ggaatgcgcc ccagcgcgtg tccttgacga
    19801 agtcgagatt ttgataagtg cgtgagacct ctttctgtgg gaagtaaatc gaaagcccgt
    19861 agcagtattg cagggcggcc tctttgtaga ctgacttcac gacgaactcg tcaggcccga
    19921 cggtcttcat cacctcttcg caggcggagc gcacctccgc gtgatcggag ttctcccgga
    19981 gcaggctgca aaaatcgtaa aggtcaacat actccgtccc gtcgaaggat tgggcctgcc
    20041 agcgcgagag cacgattgac ttgatcatcc cggcctcagg caggcgttcg agcagcgccc
    20101 cggccagggc gtcaacggag cgggcgagcg cgtcgcactt gccgaggtcg cagaccgcct
    20161 gcgtgacact gacgtcttca taatcggcgt aggaggcgag gtacttctcg gcgacgagac
    20221 aggcgaggcg ctcaggctcc atttcgggac gggcgacaag ctcgccgaga atcctgttgt
    20281 aaggccagcc gtcgagcggc gcgacgcctt cgggcgcgac ggtgaagcgc gccccgccgc
    20341 gcacctggta acacacctcc gcgccgctca ttaggcaggc gtccatgccg agaacgtcca
    20401 cctcgcgccc gatgatttcg cgcgccgcgt cgagcgcctg cttgaacttc cgcgtgtcga
    20461 gaaagtccac ggtgtcggag tcgaggacgg ccgcgtggct gatgccgatg ccgcgcgttg
    20521 gttggccgca tccctcgtcc gcgtcggggc ggtgcgggtg gctcgcggcg ttgggcacgt
    20581 tctcgtcgtc cgccccgttg ccgtggcccc acatgacgag catgtagcgc cgggccgggt
    20641 agttctcgac gccccagcgg acgaacgcct caagcgagcg cgggtcggcg gtgtccgtct
    20701 cgcccaagtc ctcgacaacg tctccggcga gggcgccgtc gcggccgtct ttcctgagat
    20761 agtaccgctt cgtcgggcgg tgcttcacgc cgcggctgaa ctgcgcgatc agggcgacct
    20821 ccgcggtcga gccggcctcc ttcatctcct tgaggtcggc gaagccgttg ttgtcgaggt
    20881 gtttgtcgcc ggccatgtag accatcaccg tccactgccg ctctttcatt acgtgcaagg
    20941 cgcttggctc cttcccgact tagttctcct tgacactatc gcttgactcg accgccggaa
    21001 gacttattga ttcgacggcc ggaaggcttc tgaattttga cagccttgtc acgccgcgcg
    21061 ccggtcttct ctttcggctc ggcgccgctc ggcgcatcag catcacagtc ctcgcactca
    21121 aaaaactccg tgggcgggtt cctcatggtg ccgatgacat tgctcaagcc tttatccact
    21181 gagctgtttt tatccactga gctgttttta tccactgagc tgttccgatg acccagcaag
    21241 atggactggc tgccgctggc gacgttgtac ttatcaatcg tggccctgcg gctgaagctt
    21301 tgcggctcgc cgcggccggg gcgcgcggcg cgcgtcgtcg ccgcgaggta ggcccggagg
    21361 aagagcgccc actcggagtt cgtggcgaag tagagtttct ggtagtcgtt caggtcggcc
    21421 gggacatccg cccacgggaa gtagatcgag acgccatagg aatactggac ggtcagcccg
    21481 ctgtagcacg acttcaggac gaagcccggc atctcgcgga agttgtcggt gtactcccgg
    21541 ccctcgaact cggaacggaa ggcggcgtcc gccttcgcgg cctccggctc gttggcgctg
    21601 ccccggatgg tgttcatcac gttctggcag gccgtctgca cgtcttcgta ctcgcagttg
    21661 gctttcagca gttggcagaa gtcgtagagg tctgtgtact gctcgcgctt gtagggctgc
    21721 gcctcccagt gcgacacgag gagcgccctt ctcacttcgg ggtcagtctc gccctcgccg
    21781 gtcttcgggt cgggcaggta accccgcaga gttctggaca ggctcttgac ggcggccatc
    21841 agatggacgc tctcgccgag gtcgcaggcc gcctgatcca ctgagacggc cgccgcccgg
    21901 tagtcgctgt agtagttgat gtagtcgcag acgattttcc gcgagagttc cttcacgtaa
    21961 tagtcgtcgt caccactctc gtctcccgcc gcgaagtccc tgaccgtctc cagcacgctc
    22021 gcgtagggcc agccggtgag cggctcgaag ccctcggcgc cgatcatcac cttcgcgtgg
    22081 ttgtgcacgc tgtaggcgac ctcggtcatg ctcatcaggc aactgtccag accgaggatg
    22141 tcgatcttcc tgccgcccag caaatcttcg ttaacccgct cgatcagttt ggccatgctg
    22201 atcacgctca gccggtctcg cgcgtccgcc aaatccttgt cgaggaagtc gccgaggatg
    22261 ccgcccccgt gtcccgacag gacgagcatg tagtgttcgg cctcgtgatt cctgatgcaa
    22321 tgtttgacga aattacaaat cccttcggag tacgacggtg gtttctcctc ggcagactct
    22381 tcgtcgatga ccacgcccga gcgtttggcc gcgcctgggc gtttggccgc gacccgcgcc
    22441 tgcgaccgct cccgcgcccg cgccctccgg gactcatcct cctcctgacc ctttcttccc
    22501 ctgttcaatt tggcgttgag gttgcccggc ttggtgtcga agtcgataac cagtctcgtg
    22561 ttctcgtgga ggccggtgtc gagctgcgcg atcacggcga tgtcgtcatc cgtcccgacc
    22621 ctacacatct cggtgaggac gaagatgcac tccgcgctca gattgttgtc gccggccaga
    22681 tgcaccatga ctgtccattt atgtttcgtt ccttcgcaca tgaggtgagt gctcctttcg
    22741 ctactcttca gactcggggc aagtgagccg aaacttcccg cccgcggacg gggttgaggc
    22801 ccgcggcaca tgtcttctat agcgttggcc gtcgctgacg gcgattgctg cccgcgacgg
    22861 ggccgcgaat tgagccacgc gcgctcggcg ctgaggccat gacttcgaag ggcgacaaac
    22921 gtctcgaatt ctcagacacg ccctcaacct ttaatgacta tatgactgga gatatttcgc
    22981 cttcgcttaa agtgcaagcg tttgggaagg tgtaaccgga gcgtcgcccg gcgcctggca
    23041 gcggcttcgg acggttggcg gattctatac caacgtgcgg ggcagtcaaa ggttttatta
    23101 accaaacgcg aaggggcgga attcggagac ttccgcgggc cgcttcgccc atcgtgcagc
    23161 gcgtcgagtt gcggtgcggg cagcttagga gtcgccgggc gtcgagcgtt tggtgagcca
    23221 ctggacgagc gggcgcaggc tctcgggcat gtcggcgtca gaggcgacca ccgtgcgagt
    23281 gtcctcatcg tcctccacta ccagacggta atggaaggcg tcggggcgtg gccgcgctgc
    23341 gccggcgcgc tccgcattct gacgactcgc ggcgctcggg atgtcggcct ccgctaaaag
    23401 tccgcggagt tcctctgcct catcggcagc gagttcgtcg gtgtccacct tgaagcttcg
    23461 tctcatcgcc ggcgcggcga aaccgccggc ccgatcaaaa ctgattcgca tgaagaactc
    23521 ccttccgttc gacgatctcg ggcgcgctcg gacgcggcgg gcggaccggc cgcacagtcc
    23581 gtacacgtct gttgttatgg agggaggcgg cgggccggcg ggccgtgcgg atgatagcac
    23641 ggcccgcgag ccaggggttg ggtgatgggg gtggggcgtg agggggtttc agaggcccgc
    23701 tcagccggcg cccgccgccg tgtgtgttca ggcgacctcg gcggccctgc ggaacacctc
    23761 gcccatctgt tcgagttgct tcttcagcgc cgctccgttc gactcgtagg gcggcttcgt
    23821 gcctcccttg cctctgctct ttgtcgcggc gggtgcggtg atgttgatgc cgacctcggc
    23881 ccacgcctcc ctgacagcct gctgctgggc gctgccagcg ccaaagacgg cgccggccac
    23941 ctgcgccgtg atgttggcgc agtcctgaaa ttgggccatc tcccagagtt ggagcagggc
    24001 gttgtaccag atgagaccgg catcctccca tgcgtagccg ccgagctggc gggcgacgag
    24061 gtagaaggcg tggttcggaa tcccggagtt gatgtgtacg ccgccccagt cgttccactc
    24121 gtcgtcgggc atgttccggt agtcgctcat gtgtttgggt tgcgggtcgc ggccccccag
    24181 ttgcgggtcg tcgtaggcgc tgccggggtc tttcatcgag cgcagggcga cgccgttgat
    24241 cccatcggcg agcagtccgg cgccgatcag ccagtcggcc tcgtcggcgg tctgtttggg
    24301 gtcggccgca tactgcttga tgagcgagcc gaagacatcg gagaacgact cgttgagcgc
    24361 gcccggctgc ttgtggtaga cgagcttggc cgtgtagtcc gtgacgccgt gcgtcagctc
    24421 gtggccgatg acatcgagcg agcgggtgaa ccccttgaag atgatgttat cgccgtcgcc
    24481 gaagaccatc tgccggccgt tccagaaggc gttgttatag ccggcgccga agtggacggt
    24541 ggcgacgagg cgcatgccgc ggtcgtcgat ggagttgcgg tcgaggactt ccgagaagaa
    24601 gtcgtaggtc gcgccgaggc cgtcgaacgc ctgcgcggcg gccccgccgc ccgacggcgg
    24661 attcgactcg tcgccgacga gcttgcccgg cagctcgaac tcgttgagga acttaccctc
    24721 ggcgtcgtgg atgacgcggg tcttctcgcc gggcgggttg gtggtcagcg cggcgcggac
    24781 gcgtccgacg atctcgcgct ggccgcggac gcgggccgag gtcaccagag tccgcaacgc
    24841 cgcctcgcga accgtggcgt ccttactctg ggccattgcc cgcagcatgt gcggggggat
    24901 gatgcagttg actgatttcc gatgggtaca catagtttcc gtctcctttc aaaagtgcag
    24961 gggcgtcggc gttcagggat ggtcgcgtcg ccgcacgcga aagagagccg cccgcccgac
    25021 agttgtatcg gcttgcggcc gacacaggct tgttgagaag ttctcaacga agtagtcggc
    25081 tcgaatgatt cgtcaggcgt cgctcccgac tctacgcatt tcaattcaag tcagacagga
    25141 aggcagagac tctgctcttc aactcttcga tcaggggttt gagtttgtct gtcactggtt
    25201 tggtgacggc cgggtcggcc ttcggcttaa ccgcgttggc gaccgcctcc gcttcggagt
    25261 ttaaagagtg aatctgagtg tcaacctctc tgacggtctc catcacggct ttgaaacggc
    25321 tcacgtcatc cgcgtcccaa aacctgtgga gcagggccgc gttgacgtac tcctgtttgc
    25381 gtaaattcac gatggcgtcg ttgtatggct tcacggccag ctcaagtgac gatttagaag
    25441 tccagccctg ttcgtaaaac tcggtgacgt tctcggcgga aaaagtgtag ttcgagaggt
    25501 cggtggcgag tttctcgtaa tgggtggttc tcaggtttgc ccggttcacg gcaaatttaa
    25561 tgttgcccac gatgctgtca gaaaaaaccg tgaggagggc gacaagcgtg gtcacgaaca
    25621 gggtagtgaa gttgtcttta attttttccc agatcgtagc catgaattac ttcctctctc
    25681 ctgcggacga ccgagccgaa agtgtgtgcg actgcgagac cctgtctgtc agaccggcag
    25741 gtcgcggccc ggcgggcacg gcttgaggac agctttcatc cccgccgcca acgcgccgat
    25801 aacctcgatc aaattttcgc cgacgaagag gcagcggccc acgggcacgc cggcgcgcgt
    25861 ggccgccagc ctgtaaatct ccgcgtcggg cttggccacg ccggcgtcgt ggtcagagat
    25921 gaacagctcg gggtcgatga actcctcaag gccggctcgg cgcagcagcg tcttcccgtc
    25981 ctcgttcgtc agccccggcc cgagcgtcgt aatcaccccg accctcacgc ccaccgcttc
    26041 gcgcatcatc cgcagaagcc gcgcgctgct cgggaaggcc accagcctcc ccgtcgccgc
    26101 gtcccgctcg ccgagcgtcc cgccgatatc gaagaacacg aaatcgatct cacgcatgcc
    26161 tgccatcgtg atgtcgtccc ctttcgcacg cggcggcggc gtgtcgcgcc taagcgttca
    26221 cgccaccagc gacgttaacg tttagccttg aaggtgagtc cgattaccat cttgtgatcg
    26281 accaatttga aggaaggcgg gacggagccg tactcgtagc cgatgaaggc gttgtagagt
    26341 ttggagaagt cgaaattcag cttcgccgtc gcgtagtcac gggggccggt gccgatttca
    26401 accggctgta gttctccgtc gtcgtcctcc tcgaacttga tctctttgcg cagcggccag
    26461 cggcggatgt aagacgcctc caagccgatg ctctgtatgg ggcctctcgc catcgggaag
    26521 atgatgttga gggtcgagcc ggcgaggggc cggatgagga atcggccttc cgcctcgtcc
    26581 agggggctga caaaattctt cccgatctcc agcccggcga agggtcggag gtagaaggtg
    26641 gtggagccac tgaggaacgt tttactgagc agcgtgaagc gagtctcgtg aacgaagttg
    26701 acgttgccga agtcgcgctc ggcctcaatc ttcggactgt tcttcaagat caggcgtctg
    26761 aaggggacgg actggttcgg gccgcgatag cgccagagcg ggaagcgcga attgaagccg
    26821 aagttcagac tgtcggggtc ggcgccgggg tcgctgctcg ccttcaactc gaagaagggg
    26881 ccgtagctgt gcacgcggtt ctcgatgacc ttcttgacgg ggtagtcgat caggacatcg
    26941 acggtgccaa agaagctctc gccgctcggc cgcgtgacct cgcccgacac gtaaaagttc
    27001 gcgtcctcgc gcccgtcggc cgccgtcagc gacaggccct cgccgctctc ggagtcgtcg
    27061 gccttgcccg tgggcggcgg ggccggctgc accgccgcct cgacgctcac aggtctttgc
    27121 agttgtttgg ccgggcacag accgctatta gtgatcgtgc tgccggacgt gctgatgaac
    27181 ctgaggttga atgcgtagag gtgatagacc ccgtctccct cgtcgagtcg cacgctcggg
    27241 tcaacggtga gcgtcacggc gttcggggtc agccggtcgg ttctgaagta gacgtagacg
    27301 attgggatgc ggcggctgtt acgcacgtcc agcaggtggt agttcgacgg gtccttgacc
    27361 gggtttcgct caagatcgag gttaaggttg gcgcgaacta cggggtctac gttgagcggg
    27421 tcgaggggtg tgttggtggg taacgagaga tcgaaactct cgctgaaggt caccgagagg
    27481 atgcgcctac cggagccgtc gcgatctaag agcgcctgcg ccagctctat cgtcaccggc
    27541 accgtattcg cgctggtgca gggcggcggc tgaccggcag ccgccaccgt ccacaacagg
    27601 cacgcgcacg cggccaacga gatgcgcgtg agcgccggcc atcgaagttt accgttcatc
    27661 gcttctttcc ccttccggcc gcgccggcct tcccagcccg cttcgcgccg cccttccgtg
    27721 ctttcttcgc gctcttcttc gcggcgccct tgccgctctt tgtcgctttc ttcttcgtag
    27781 cgccggctcg gacgctgctc ttcgccgggg ccgcgcccct ggtcggagcc gccagtccgc
    27841 gccctgcttt cttcgctccc gccttggcgg ccttggcggc gacttggacg gcctcggagt
    27901 ttgcaatcac ctgaacttgc gtctgggcga cggtggtgcc tgtcttcagg tccaacgact
    27961 cggggtcgtc aattttgtgc tgcttgctcg ggacgccgac ttgattgttg gtcacaagct
    28021 cgtcgatcag ggcgtcgatg gccaagtcgt ctacgacgcc cacctgactg agtttattac
    28081 cgccgctgat taacccgtcc catcccactg ccgagcgaat acacgcatcg acgatctctt
    28141 cggcttccac ggatagaagt gtcatgtttc tccttatcag ggcgcggcgg gacggcgtgg
    28201 ccggggcggc ccgaccgtct ccgagcgcgt agagttttca ggtcagactc cgtttcaggg
    28261 ctacgagttc agaatgttga gcgcggcgac cacggagccg cgcagggcgt cgtcgcggac
    28321 ggggctgccg gtcttgtcgt gcgcttcggc gagggacttc gcggtggcgc cgggcgcggc
    28381 tgagacgagg gcggcgcggg cgttcttgag tttctcttcg agggcctcgc gcgactgctg
    28441 ggactgctcc ttggcgtgct ggatggcgtc ggcgcgttgc gtctgccggc cgatctcgtc
    28501 cttgagcgtc ttctcgttct gctgggcggg gagccggccg aagtagtagc cgacgatgac
    28561 gaagatgatc ggttcgaggc gggccaccat tcgctcgtcg gacggcggct tgagccatcc
    28621 cgtgagcgcc gcgccgtaga agaggatgaa cagggcagtg agcgccatca tcgtccagcc
    28681 cttgagcgtg tccgcccgct cctgccccgg cggggtgttc ccggctgcct ttgctgccat
    28741 caatctctcc agactcgcct gcttaccggc gccagaaatc cgttgccaaa ccggcggcgg
    28801 acgctatata aatgctcatg tcatcgaagg gacggcatgg ggacggagtg attgtcgcgg
    28861 gtgcgcggct cggccaactc cgtttggtcg ccctcatttc ttaacgcccc gatctggtct
    28921 gaaaactatc accctaattg gtttttcttt ggcctcgcct caacaatccg acccgtccgc
    28981 gtacacgtcc gcgcgagggc cgcacgaacg tgtcactcag agagcgctcc acgaggcaac
    29041 gcctttttct ttttgcccgt cgaacggccc agacacctcg ccccacctag cagtccgacg
    29101 ccttgatggt tcgcccccgg aagtgatgta acggcatgcc ctcaccctca gagatcacca
    29161 agctgctggt cggttggtcg aacggcgatc aggcggcgtt ggagagtctg ctgccgctcg
    29221 tcgagggtga gctgcaccgg ctcgcgcatc actacatggg ccgcgagcac gccggccaca
    29281 cgctacagac gaccgccctc atcaacgaga ccttcctcaa gctcgtcgat cagagtcgcg
    29341 tgcgctggca gaaccgcgcg cacttcttcg gcatcgccgc gcagatcatg cgccgcatcc
    29401 tcatcaacta tgcgcgcgat cagcggcgcg agaagcgggg cggcggagcc ttgcgtgtgt
    29461 cgctctccga ggccgacgcc gtctgggccg agaagtcgtg cgagctaatc gcgctcgacg
    29521 acgcgctcaa gcggctggcc gacttcgacg agcgcaagag ccgcgtcgtc gaactgcgct
    29581 acttcggcgg cctctccgtc gaggagacgg ccgaagtgct cggcgtctcg ggcgtcacgg
    29641 tgatgcgcga ctggaacatg gcgcgggcgt ggctcgcgcg cgagatcgag gatggaagtt
    29701 aaagactggc aacacgtcga gtcgctcttc cacgccgcgc tcggcctgag agcgggcgag
    29761 cgggccgcgc tgctcgcgcg cgagtgcgcc ggcgacgcgg ccctgcgggg cgaggtcgag
    29821 tcgctgctcg cggctttcga gggtcaccgc gaccttctgg aggagccagc cttcagtctc
    29881 ggactgaaag tgatgtcgga agacgccgtc tccgcgtcgc tggtgggtat gcaggtcggc
    29941 ccctacaagc tcatggaagc tctcggcagg ggcgggatgg gcgaggtcta tctggctgag
    30001 gacacgaggc tcggccgcaa ggtcgcgctg aagtcactct cgggcaggtt ggtcggcgac
    30061 aactgggcga ggcggcaact gatccgcgag gcgcaggccg tcgccatgct cgaccacccg
    30121 aacatctgcg ccgtccacgg tatcgaggaa caggacggcc acagcttcat catcatgcag
    30181 tacgtcgagg gcgagaatct ctcgcgcctg attagcgagc gcccccccga cgccaagcgc
    30241 gcgctcgcac tggccttgca ggtggtcggc gcgctcgccg aggcccacgc ccacggcatc
    30301 attcaccgcg acatcaagcc gcagaacgtc atggtgaacg ccgacggaca ggcgaaggtg
    30361 ctcgacttcg ggctggcgaa gctcgtccgc ccgcagagcg cgctcggcgc cgtggacagc
    30421 ccgagccagg cctcgcagag cgggctggtg atgggcaccg tcgcctacat gtcgccggag
    30481 cagttgcgcg ccgagcgcct cgactttcgc agcgacatct tcagtctcgg cacagtcctc
    30541 tatgaactgc tctcggggaa gaatcccttc gcgcgcacga gcgacgccga gaccatctcg
    30601 gccatcctca cctgccggcc ggcgccgctc acgcaggcca cctctgaggt ttcgccggag
    30661 ctgagccgca tcgtcctcaa gtgtctggag aaggacaggg agcagcgcta ccactcggcg
    30721 agcgaactgc tctacgaact cagcaacctc cagacggccg gccacacggc cccgcgcgga
    30781 agggcgcact ttgcgcgccc gcacttcacg tcgcgcgcca cgcgcgccgc cgccgccatt
    30841 ttcctgctcg tcttgctcgt ctccacattc ctgtggctct cctacgcccg cctgacgcga
    30901 gttcagaacc tcgcggtgct ccccatcgtc aacgaaagcg gcgacgcgag ttttgattat
    30961 ctgggcgatg gcctgacgga gagtttgatt aaaaagctct ccggcctgtc caagctgcgg
    31021 atcaaggcgt tcacgaccgt cgccgtctac agaggccggg agtttgaccc gcgacaggtt
    31081 ggccgcgaac tgcgagttga tgcggtgctc atggggaggc tcgtccggca gggcgaatcg
    31141 ctggtgctgc aaacccggct cgtggacgcg gcgaacggtt cacagttgtg ggatgcgaag
    31201 tacgacgtga ggctggaaca ggttttcgag ttacagaatc aaatctcgga aaacgtcgcc
    31261 gccggtttgg agttgaggct ggggctggag gagaagaata gtctggccgc gcatcgggcg
    31321 agtcccgagg catttaacca ctacatgctc ggccgctact actggaacaa ccgaaaccct
    31381 gagaacatcc aacgggccat caaacacttt gaggaagcct tcgtcctcga agcgaattac
    31441 gccgaggccc acgccggcct cgccgattgc tacgtcttcc tcgcctccgc cgcctacggt
    31501 gagatgacga ccgccgacgc catgagaaag gcgaagggcg cggcgaactt agccctcaag
    31561 atcgacgagc gcctgcccga ggcgcacacc tccgtcggcg tcgttcagtt gaggtacgac
    31621 tggacatggg acgaggccga gcgagagttc aagcgggcca tcgagcttaa ccccgactat
    31681 gcgccggccc actactggta ctcgaacctg ctggcgatca ccggccgtct cgaagacgcc
    31741 atcgcggaga gcgagagggc caaggctctc gacccatcgt ccccgtccgt gagcctgaac
    31801 cgttgccgcg cgttcttcct cgcccgccag cacgaccggg ccgccgcgtg cttcaatgag
    31861 atgctcgata aggagcccgg caacgagcgc gtgcaataca ttctcgggct cgtctaccag
    31921 cagaagggga tgtacgaaga ggccatcaaa atacacgaga agattgcgga gacgaatctg
    31981 aggctggcgg cgggcgcgct cggctactcg tacggcagga cgggcaggcg ggaagaggcg
    32041 ctgaagatgt tggacaaaat aaacgagctg tcgaaacaaa agaccgtgcc cgtgatggag
    32101 acggccatca tttacgtcgg gcttggggac aaaaacaacg cgttcgcgtg gctggagaag
    32161 gcttacgagg agcgtttcgc gtcactcacc tacctggcgg tcgagccgct cttcgacagc
    32221 ctccgcgcgg acgccagata tcactccctc ctgcgtcgcc tcaacctccc gccggcgaga
    32281 tgacctgacc ttgcgaggtc tcgaacccct cttcgtcgcg accgcgccgc ccgcttcaca
    32341 cgccttcctc aacgcctcgc ttccgaccta cggacgagcc gcggcccgcc agcacgggtc
    32401 gagtcgcgga acggggtgga gggccgagcg ccttgagtct ttcggcgacc atctgcgcgc
    32461 agggccgtcg ccgggggtct gcgctcaaca gcgcctcgac gagccggctc acccctgtgg
    32521 gaatgttccg cctcagcttc gccaacggtg acgagctggc ggcgggcggt cgccccgtca
    32581 gtaaaaaata gataaccgcc cccagggcgt agaggtcgtc acaaactctc gcccgcccgc
    32641 cccccgtggc ccgactctcc ggcggggcga attcgggggt gctccagggc accgggtcgg
    32701 gttcgtctac ggggcaggcg ccttcaaagt ccagcggccg aagagtccct ttcttcgtga
    32761 ccatcacgtt agatggcttg cagtccctcc acacccagcc ggcggagtgg atttgagaaa
    32821 cgagtgtggc aagctggacg ccgtagcgga gcacacggga gactgacatc cgccgcttca
    32881 gcccttgaag tatgttctgg agactttcac cctcgatgaa ttcggtcaca agatagtagt
    32941 tgccgcccgc ctcgaacgac gcgtagacgc gcggcgcgtc tatccggccg gcgcgcagga
    33001 gcttgagcac gcgctcctcc tgcctgaccc tccagtggcc gtcacgcccg tcccacgcag
    33061 gctcgccgtc cctgcgccct tccttgagga cgcacaggcg cggcgtctgc gtggtcaggt
    33121 caacggcctg atagacgccc cccttccctc tctgtgtcag cgagcgaaac gcccggaagg
    33181 aagtgcggag cggactctcg ggcgcacgcg cctccggccg cggccaaccg gcgatcagag
    33241 gatcgccgac ccacgccggc ttcgcctcca ccgactcgcg caggtcgggg accagatttc
    33301 cttgcgggtc gcgcatggcg agcgtgctcg tgccgtcggg gtgttctatt ctcagaggga
    33361 cgaaagcgcc atagcggtaa tagacgttgc tgtcgggcct gaaccttaga tcaaagggga
    33421 tggccggggc gctcagcccg cgggtcagcc tgtgcagacg ccccgccagc aatctggcct
    33481 ccgcgtcgga ctgcggatag acggtgatga actttccaat ctggctgtac ccgtgataca
    33541 gcccggagtt gatcctctcc aactcttgca gggtggcggg agccttgaac aaaactccgc
    33601 ggccactcag aaagggggcc gccctttcaa acaccacgtt cgccgtgagg atcgtcgccg
    33661 agacgtgtag cttccagccc tgctcggggt cggcgggggc cggcgcgcga ctgtatctcc
    33721 agatggaggc ctccgccttc accggaaggt agctcgcgca cagcccccgc cacttctcgc
    33781 tcgatgactc gggcagggtt ccgaaagggt tgtggacgat gtcgatctca gcgcgcattc
    33841 ttcaaaaccc catccttgaa aaccgcactc ttacagagat ccgtgttcgc cgatggcgcg
    33901 cgggcggggt aaagtctgac ggcgcatggg gcatcggcgc gcaaggattt gaacccccgc
    33961 cacgtggtgc aaaatatgcc gcgggagcta atgacacccc cgcttattag gcgaggcgtc
    34021 ttagccggct cctgttccta caaattcaac ttgttttcca cggtatggag aagtcccctc
    34081 gtggtgcccg acccgcaagt cccgttggcg acgaggtaag ccctcgcttt ttgtttagtc
    34141 tcgtcggcgg tgccgtcggt gggtatcaac cgggccaact cctccggggt cttcgcaaag
    34201 agcttcatgt acgccgccca ttccgcggcc ccgccgagga tcgccgcgac ggccttgtca
    34261 cctacttcag tcgccacctc cttatcctcc aagccggcgt cgacctccgc gatgatctta
    34321 gttatcactt cctctatgtt gctcttgatt gtcatgactg ttctcctttc ggcgctcacc
    34381 ggcgccacgg ggttagcact tacgtcgtca gaacccacac acctcaagac gggcagacgg
    34441 tctgcctcaa cagggctgcc ggaagtagtt tcactcggct aaaacgcccg cgaggcgtag
    34501 aaggtctggc agggtgaact gccctggcgt cgggccgagc gtcggggccc agtcttgctc
    34561 cttcagaatc gagtcgtggc tccggcgcac cagcccgatc agcaccgacg cgaccagcgt
    34621 agtgccgacc gggccgaggg agtttcctga gccgaatttc gccgcctcgg ccaggatgta
    34681 aaaccacagc ggggtgcggc ctgagaaacc taagtccgct aacacctgcg cctgcttgtg
    34741 gctgacctcg tcggcgacgc cttcaatgtc ggccgcggac atgcggtgct cggggggaag
    34801 gccgagagcc tgtgcgacag cctggccggt cggcattctg aggacgtagc cgcgcagaag
    34861 gtttcggacg gccaatctgg tttcgaactt cacgagcatt ccgttctcat cccgcaggaa
    34921 ttcgagcggc tcgacgagct gcgtgtcgag ccggcgggcg acgttgactc ccccgcgcac
    34981 gaaattcttc cagcggatga cccagttccc gaggagattg tggtagtcgt ccagcgcatg
    35041 ggacatgaga gtgaacccga acggcgtatc tgtctgctct tcattgaggt cgtaggttct
    35101 cctgaccatg ctgtgcccga agcggaaggc ggcgacggtg aactccaacg ggatgaagaa
    35161 gctgtcgtcg ggcgggtcgt agaacttctt cgggtcggcc cggactttgg cgagcgtttc
    35221 ggggtgtcca atccggtcga ggaagtcgcc gatcacgatc cactggtagt gctgtcggag
    35281 gagccggctc gccgcttcaa agtcgttccc taaatcgacg atggcgttgt gcgcgcgcag
    35341 gaaggccacg tgcatttgcg agaccatcac gttctcgtcg tttcttccgt cgccgatctt
    35401 ggccgcgcca ttaagaagcg ggtcgaactc tcgggggaga tcgttgaagt agtcatgccc
    35461 ttcgaggggc gtccccgcca ccatttgaag cagcatcttc tccccctggc ggggaggcgg
    35521 ctggcagtcg gtgtcgttgc agtagctgcc gtagacgctg tccaggtcta agatggccgt
    35581 gcggatgttc tttatttgac gcgcctcgtc cagcggcaga ggcgcgaggt tgtcgtcgtt
    35641 caggggaata tctctcgtca ccgcctcgaa ggtgatgtcg tgatccacga actgaccgaa
    35701 gtaggtgtag gcagacggga tgttggagtc ggactgttgg ccgggcgctt cgctcatgga
    35761 cttaccgagt tctctcaggt tcttcaccgt gtcggggtgt tcgggcagcc gactctgggg
    35821 gtcattctgg agatcaggaa acagataatc gaagcgggtc aggacgagcg gggccacgtt
    35881 gatgacctga cggccgaagc tggcgttgat cagatgaatg ctcttgaggg cggcatcaac
    35941 ctgagcgagc gtcaccacct tgccgtgtga gatgcggggc attgtcgttt ctcctgagtg
    36001 ggcacacgcc gggaggcgtc tgctccttcg cgctggggcc gtcgagtctt gcttccgaac
    36061 cctccactgg cctgaaagcc cgtggggtcg gctccgaagc ctttcgttgc tggcattagt
    36121 taacgcacca gggaggcccg gaaactatca ccagagccgg gacgcgtcat cgggcgcggc
    36181 gcaccatacc acaaaagcgg acacccactg tcagctttga gcaggcagct cgataatcct
    36241 aatgcggggc gcgggcaaaa aaatagggcg accgcgcggc cgccctcaag tttggaggga
    36301 caaacagacc tttgtttcgc cggccctaga ggctgcggcg gaagaggtag gacatcttga
    36361 tgaagaaggt gcggccgttg cgtcggaagc cgggttcgag gtcgcccgag taggggttga
    36421 cgaagccgcg gttgaggtcg tcgttgtagc cggcgtagag cgcggtgccg gggttgggcg
    36481 tccagcccaa aaggaactgc ccgcgcgcgt tcgagttcag tgtgttgtag tcgatgcggg
    36541 cgcgggcgaa ggcgaagcgc gtgaactgat aggtggtgcg caggctgtag atggtcgtgt
    36601 cgaaggcgac gaggcccgtg tcgtggcgca ccagacggtt cttgttgaag ctgacgctca
    36661 tgttgagatc gtcggtcgcc ttgtaggtca gtccgccgtt cgtgtagagg aagtcgcccg
    36721 cgccggggtc gagcggcgcg ccgcagacgg ccgccggctc ttcctcggcg caacgccccg
    36781 actcttcggc ctcgcgcgcg cggacggccg cctggctggc gcgcgggaag cgggggccgg
    36841 cgccgaagtc gtagtcgagc tggccccagc ggtagccgaa gaagtagttg accgaaatcc
    36901 tcttggtcgg cgtcgaaccc atgtaggcga aaaagttctt cgagtagtcg gaatgctcgg
    36961 ggcggcccga ggcgaaggcc gtctcgccga actcgtaggc gaagacgcgc tcgtagccgg
    37021 cgttgaagcc ggtgcggaag tatgtctgcc gttggagatt ggggccgacc tgcccctcgt
    37081 aagtccagtt ctggagacgg ccctgccagt cgaagttgcc gccgaagtag tggtaggtgt
    37141 tccaggagat gatggtcgcc ttcggcttcg gctcggtgaa gtactcgaag ttgaggttgt
    37201 ggttgttggt gttgaggcgg cgggtgaagc ccacgtcggc gcggtagccg gaggtgcgcc
    37261 ccgagccgtt gacgttcatc cacgtgtggc gcgcgctgcg cgtgacgttg taggcgtagg
    37321 cgaagccgtt gagcgtgcgg caggtgtcgt cggccgcttc ggcggcgaag tcgcaccggc
    37381 gggagttggt gccaagcacc tgaaactcgg ccgtcgtcat cttgtcgaag cggaagcggc
    37441 cgtcgaagcc gccgacgtag ttgctgcggt cggcgaagtt gtaggcggtg gcgagcaagc
    37501 ccagatggtt ctccttgccc acgtcgcgct tgagacggag gatgccgacg gtggcgttct
    37561 ggccgtcgag gcggtcgatg gcggcgggca ggttgtcggg gtcgtagcgc tgatcttcgg
    37621 tgtagttgcc gggggcgttg tcggtggcgt agaggattcc gaaggtggtc ttgccgtgtt
    37681 tgccggagag tttggcggcg atgtcggggt caacgatggt gcgcgtgtgg acggcctgaa
    37741 tgggcgtgcg gaaaatctcg atgccttcga ggaagaaggg gcgcttctcc tcgaagaaga
    37801 tggggaagcg ctggttggcc gtattcaccg tggcgtcggc ttcgacctgt gcgaagtcgg
    37861 ggttgagggc gaggtctagg gtgagggtcg gcgtgatgcc ccacttggcc gtcagtccgg
    37921 ggtcgaggtc aatgggctgg ttgatgaagc ggccctgatc ggcgacgccg gtcaggcgcg
    37981 attgcgagcg cgtcatcgag cggatgcgct tgcccgtctc ggagacggtc agactcggga
    38041 tgagttcgag cgtgcgctcg gtggagatgc cttcgaggcc ggtgaggtgt ccggcctgat
    38101 tgagcagccc cgaccggtcg cgcgagatgg gcatccacga gtcgatctcg tcgttgttgc
    38161 ggtcgatgtt gcgccagatg tggagacccc agagcttgtc cttgccggcg acgtagcgca
    38221 gcgacttgaa ggggatggcg acttcgagcg tccaacccgt ctcgccgatg acgcccttcg
    38281 actccatgac gatgtccacg ctgaagtcgt cgttctgccc ctcggtgcgg atgccgtcct
    38341 gctggatgcc gagcggattg aagccgagga tgtaagcctt gcgctggtcg ttgtaggtgt
    38401 cgatgaagaa gcggatgttg tcttcgccga agacgttgtc gcgcttggcg acggtggcgc
    38461 gcaccttgtc cggctcgtcg gtggcgcgca cgccgaggta gagattttta gcgtcgtagc
    38521 cgatgaagac ttccgtgggc tgcgagggcg cgatgttgtc gccggggttg gtctggtaga
    38581 aatccttgaa gacggccgcc gtcttccaca cctcctcgtc gagcttgcca tcgacggccg
    38641 gcgccttgtc gaacttgacg atccgcacgg gctgcttctt ctcgatgggg agaacgatgg
    38701 ccgccttggc gccgccggcc ttcacgtcct gcttcttcgc cggggggtcg ctcggcgcgg
    38761 ccgccgaggc gacttcgggc gacggggacg ggacggggcg cgcgtgcgtc agttgggtgg
    38821 cggcgcagag ggcgacgact gagagcagca gcttttttat cataatcaat tctcgactcc
    38881 gaaagcgttt gcgggaaggc gcggggcgtt tttgagacgc aactgcccct cacctgacca
    38941 cgccgcaccc ggcgcgcgtc aggccgaatc tcttccgttt gttcgcttgt ggcggcgggt
    39001 ttaaagcgtt aggggagatg cgctccgacg agtcttaggc cgcttctcaa gaccgtcccg
    39061 gttttgtttt ttatcctgtc tgaagtggaa tgaaagtgcg cggcgacaga acacgcgcac
    39121 cactcgaata acgcccgccc ccgcggtgaa gtttcgccac ctcaccgggg ttctgtaaaa
    39181 agcgtgtaaa aaacccggct gagggtggtc aacccctgga gcctgaggtc cagcccactg
    39241 actaataccc cgaaccggcg ttcgccgttg agaacccggt cggcgaaaag tccctcgaac
    39301 ttcgccgtcg agccgtcctc atagaccttc gtcgcgctga gtcgcgcgcc gtcgagccgg
    39361 atgtcacgga gttcggcgcg gcgcgcgcct tcgcggctcg tgatcttcag acgcccgtcc
    39421 gcttccgtac cgacttcgag gacgtaagag tcgtcgatgc cgatgtagcg ccccgcgtag
    39481 tcgcgcgcgt ccacgcggac gaccgcctcg aaacgcttct ccttgtgctt gtgttctctg
    39541 tgcttgtgtt tttgcggcga cccttcggcc gtcgcggcga gcaggagcac cagcagtaac
    39601 agccccgcgg ccagctcgaa tgtattagac cttctcatca cttcggcccc cttcgccgtc
    39661 tatcaaatta aacaccgctc gtcggggctt cgtggcaaca tttttcgccc ggcgctcggc
    39721 cgcctcaaat cgacggggcg gccgagcgtc gcccgacgct ttatttcgac aacacctcgc
    39781 ggaagtattc cagcgcgcgc gggtcgcccg actcgccgag ccagtggatg gcggccttgc
    39841 gtacttctgc gttccggtgc gtcttggcga tctggataag gagcggcacg gcctgctcgg
    39901 gcggcttctc gctgatggcg tgcaccgcct gcttctgcac ctcggtgtcg gcgtcggacg
    39961 cgttgacggt gtccttcagg aagtcgaggc tgcgcttgct ggccttctcg ccgatccagt
    40021 gaattgcctg gcggcgcagg tcactgttat cagcgcggcg cgcgacctcg aagagtttgt
    40081 cttcagcgcg ctggctgtcc atctcggaga gggagtggag aatctggcgc cgcacgtctt
    40141 cgttgcggtc ggcgtcgaag atgcggacga gttcgtccaa ggccgactcg ccgcgctcgc
    40201 cgagctggtg gatggcgtgg cggcgcacgt cgccgggctg actctggtcg cgcgcgatct
    40261 caacaattcg gctttgcgcg cgcggactct ccatctccga gaaggcgtgg aggatggcgc
    40321 gcttcacgtc aacgttttgc tcctgcgagt agaggcgcat caactcgtca accatcgcct
    40381 cggtgttgcg ctcgccgagt tggtgaatgg cctgcttgcg aacgtcgggc ggttcggccg
    40441 tgcgcgccag ctccaggagt tttgcctcgg cgcgcgggct gcggtgctcg gcgagcgcgt
    40501 ggatgaccgt gcgcttcacg tcctcgctgc ggtcgttcgc gaagatgctc atcaggtcgt
    40561 caacggcccc gtcgcgctcc gactcgccga tgatgtggac ggccgtgcgg cgcgcgtccg
    40621 agtccgtgtc gctcttggcc accttgatga cgaaggcata agcctcttcg cggttctcgt
    40681 tgtcgttaac ggcgtggagg atgccgcgct tcacgtcctc gggcagggcg gcgttgtaga
    40741 gagtctggag cgtggagagc gcctcgcggt cgcggctcgc gccgatggcg tgcgcggccg
    40801 tgcggcgcag gtcggtcttc tcctgattgc cgcggacgag ggcggcaagg aactcgatct
    40861 cgccgcccgt gtgaccaagc cagtagaccg cggccgagcg cacgttctcg ctcttcgagg
    40921 tgcggatgaa agttttgagg atgtcgggga cgcgccggtc gtcgtgcagg gcgatggcga
    40981 gcgtggcgcg ctcttgcaat cgcgggtctc cgctctgctc ggcgagcgga cgcaggtaag
    41041 cgaggctctc ctcgttgccc gcgcggcccg cgaagtagac gggatagccg ctgtactcac
    41101 gcgcgcggtc gaggttgtag acctccatgc gggtcagctt ccccgacgcg gggtcgcgca
    41161 ggacgaagag gccgaggttg cgcgtctcgg ccgtggtgcc ggcgcgcgtc gtgccgatga
    41221 agacgtggat gccgcccatc gtgttcatcg agccgtggaa ctcgccgccg ccgggatcga
    41281 cggcgacgcc ggggcgcacg tcgaacgtgt aggccgtcca gaagggcgcg cgtggcgaag
    41341 aggtgcgcgc ctgacgcgac gccgcttcga gcttggcggc gaggtccgcg ccctcgacgt
    41401 tgacgaaggg cggcgcctgc cgcgcggtct gcggcgactg ctccgtggcg gcctccacgg
    41461 actcggccga gagcgcggcg ccgtgcccgc gagcgcaggc cgcgccggtg aaagcgaggg
    41521 ccgcgaggag agagatggtg gcggtcgttt ttcgcataac ggaattcctt tacgaggtta
    41581 ggggtcttgc cttcgggatt gggcttcggc cgctacccgc gagttcacaa aacggtattc
    41641 acatggatgg acaggatgag taggataaga agagttaaga tttcaaatct caaatttcaa
    41701 atctgaaatc aattctttcc tgctttcatc ctgttccgtc ctgtccatcc atgttaattc
    41761 ttccggcccg gcacgtggcc cgaggccggc gcgtttacgg cttcaacaaa tcttcgagga
    41821 acttgagcgc gtcggggtcg tccgactcgc cgatccagtg gatggcctgc ttgcgcagct
    41881 cgacggagga gtcgcggcgc gccacgtcca tcagcttctg gagcgcgcgc ttgtgcttcg
    41941 actcgctgaa ggcgtgcagg agctggcgct tgacctcgtg ctgcgtctcg gcgtcgtaca
    42001 tcgagatgag catgtcgagc gtctgcgcgt cgtcgccctc ggcgagctgg tggatggccg
    42061 cgcgccgcaa ctccacgtcg tcgcccgtgc gggcggcctc gatgagcttc tggcgcgcgc
    42121 gcggactctt catctccgag aaggcgtgga tgatctgacg acggatttcg ttgttgcgct
    42181 ccgacgcgta aatccgcatc aactcgtcta aggccgactc gccgcgctcg ccgagctggt
    42241 ggatggcctg ccggcgcacc tcgacgctct cgctggcgct acgcgcgatc tcgctgatct
    42301 tcgcctgtgc gcgcgggtcg tccatctcgg agagggcgtg gatgatctgc cgcttgatat
    42361 cgacgttgct gtccgcgccg taaatctgcg ccagactgtc gaaggccgcg ggcgacttct
    42421 gctcgccgag ccagtggacg gcctgccggc gcatatcgat gtgctcgccg gggctgcggg
    42481 cgatctcgac gagcttggag tgcgcgcgcg cgtccttcgt gtcgaagaag ccgtgaatga
    42541 tctgccgctt gatatcgatg ctcggctcgg agttgtagag ctgcaccagc tcgtcgaagg
    42601 cggccggagt ttcccgctcg ccgatccagt ggatagccgc gcgccgcagg tcgtccgact
    42661 ccgtgcggct gcgcgcgatg gcgaggagct tggcgtgcgc gcgcggaacc tcgtccatct
    42721 cgaagaaggc gtggagcatt tgccgcttaa cgtcggagtt gcgctcggcg tcgtagagct
    42781 ggatgagcgg gtcgagcacg gcctcgccgc cctcgttggc gaggctgtgg atggcgcggg
    42841 cgcgcagctt ctcgtccgcc tggctgcgcg cgatgtcgag caggaacggg atcgcctgcg
    42901 ctccgccgtg cgcgccgacg agcgagacgg ccgcctccat caggcgtggg ccggcggtcg
    42961 agccgggctt gatgatttcg cggacgtagg tcatcgcgcg ctcggggttg gactggaaga
    43021 gactttgcag agcgacgatc ttgatctcct cctgctcctg cgagtcgttg agggtgttct
    43081 gggcgatgcc gccctcgccg agggtgccgg cgatctcgtt gagcatggcg cgcgcatcgt
    43141 cggcccagga cgactgcgcg tgcttcttga gcaggcgctg gagctgcgcg gcggcgccgc
    43201 gcgggtcgcc ctgcttattc agggagtagg cgagccagta gagcgccacg tccacgtccc
    43261 tgtccttcgg gtaggcgttg acgaactgcg agaacttctc ggcggccttc gcccactgct
    43321 cggcgtcgat gaggtcgcgc ccctcgcgca gcgcgcgcat ggcgggcgtg ttcgaggtct
    43381 ggacgaactg gttgaacttc ttgaggtcct gatccgtccc gtcggccttg gtggctgcgc
    43441 tgccggcgcc cgtggccgag aggccgaggg cggcgacggc gacgaggagc gcgcgggcgt
    43501 ttgggcgaac ctgtttgagt gcgttgaggg cgtatgacat tttcttctcg gctttcttcg
    43561 agtggtttat tgataagtgc gcggcacggc cgcgatgttg gcctggagga cggccacgat
    43621 gttcttccgg cgcatgcgct cctgaatgac gctcacgtcc tcgcggctgg cgcgctcggg
    43681 gagattggcg atgtcaacga gcagcggctc caaactgtcg agcgcgtgct cgacgggcgc
    43741 gttgcccgcg cgcgccgcct cgcggcggag cacgatgttg cggtagagga gctgctgcga
    43801 gcgccggcgc tcgtcggcga ttgcgccggg ccggtcgccg gggcgcgcgt tgcggatagt
    43861 gcgcatgagt atctgcgcct gttcgaggtg gcgcgccgtg tcgccccgcg gctcagcgtc
    43921 tacactcagc gccgactcgt cgcccgcatg gccgtcggcc acgtcgaaga gcggcttgaa
    43981 ctcggcgaga gcatccgcgg ccgactcact gcgcgccgcc gccgcgctgg atgcgctctc
    44041 caacgtcgcg cccccggcgg gggtcgccgt gcgcgttgtg tggcgcgcga cgatgtcttc
    44101 tcgccgctca atcgcgcgcg gcacgttgaa caactttcgg ccgggcgcat tcacgccgcc
    44161 cggcgcgtcc ttcttcggcg ccggttctga agggccgccc ggcgccggcg cgttcgacgc
    44221 aggagcgtca ggggttgagg cgtccgggtt tgaagctttc ggcgcgggcg cgtccgattg
    44281 agtaggcccg tttgagctac ggcgcgcttt gtcttgtccc gtcccttcgg ccacgagcct
    44341 ctgccccgcg tcgcggcggc tcacagacca gacgacgccg agcgcgacgg ccagcaggag
    44401 gacggccagc gccgccccga gcgccggggt cagacgcggc cataccagca gttcgcgcca
    44461 cgacgtgcgg ggcactgtag tcgccgccgc gtccgaagcg tcgcgcgtgg agtgtgccgc
    44521 cgaggcgaga atcttttcga gcgcgcggcg ctcatccgtg gcgtccgccg ccagcgggta
    44581 ggagttgacc accgagcgca gagagagaaa gtcttctttc gtgcgccggc agcccgcgca
    44641 tgattcgagg tgcgcttcgg tcgcgcgcct ctcctgcggc ggcaactcgc cgtcgatgag
    44701 gagagagatt ttttccgccc agtcgcagtt cataagatca acttcggcaa tccgctcaga
    44761 ctcgcctcgc cggccgcccc ggcggccagc gtcttctgcg cgcgcgccca acgctccctc
    44821 aaactgctgc gcgcgcggaa cacgtcaacc ttgaccttcc cttcggtcac cccgagcacg
    44881 tcggcgatct cgcggtagct catctcctcc tgctcgcgca aaagaagcgc cgcgcgctgg
    44941 tgttcgggta gctgtttcaa catctccagg acaattttcc ggcgctcgtt catctcaagc
    45001 tcttgctcgg ggtcgtgccc ccggcggccg agcgcctcga ccacccggcc gaagaggcgc
    45061 tggcgcgtcc ccgcgcgact caactcgttt aaggccagat tgcgcgcaac ccggtagagc
    45121 cagaagcgcg cctcgcccgc ggggaaccgc tccaatcctt cgttgtgaag ccgcagaaaa
    45181 ctctcctgcg cgatgtcctg agccgaggcg cgcgccgccc ctcccgccag catgcactcc
    45241 aggaaccgac agaggctcgg ataggtttca tggaaaaaac tttcaaactc ttccgtatcg
    45301 cgcgccataa aatcgcttca cgtcactaaa acagacgagc cgggggaaag ttacaaaaat
    45361 agttcgcccg cccgagcgcc gccgcgcggc cgcgcggcca agtctgacac agcgggcgcg
    45421 actttccgaa gtgattcggc gagtaagagt cagttgcaaa acctctcggc gttcgtcacg
    45481 actgtagggg cagggcaagt ccgcggcccg cgagttatgg aagcaattaa catggatgga
    45541 caggatgggc aggatgaaga acagatgatt tcagatttca gatttcaaat cttcatcatc
    45601 ctgtccatcc atgttaatta ttccttcgga aagacgggcc aaggggcgcg aaacgcgggc
    45661 gcacgggcac atgggcctct ggaagaagca ggagtttggg tgtttctgcc cattgagatt
    45721 tgtcttgcgg aaaggcgaaa gcgcatgcgg cagggagaca gttcaggccc gttggtgttg
    45781 gtggtcgatg actacgagga cacgcgcgtg ttgatccggc gacagttgga gctgggcggc
    45841 ttccgcgtcg tcgaggcgtc ggacgggcgc gaggccgtcg agcgcgccgc aagccaccgc
    45901 cccgccgtca tcctcatgga cttgaacatg cccgtcctcg acggcttcac ggcggcgctg
    45961 cgcatccgcg agcgcgagga gacgcgcggc gtgcccatcg tcgcgctcac cgcctacgac
    46021 acggcggagt cgcgcgccgc cgcccgcgcc gtcgggtgca gcgaatacgt tgccaagccc
    46081 gtcgccttcg acgcgttgct cctcctcgtc gcgcgcctca cgcagggaag ccgcgtcgaa
    46141 gcaccggggg cggagccggt ctaagacgaa gccctccccg aactcatcgc ccaaatgaag
    46201 acggccgctc caccgatggg gcggccgttt tcacgcgtcg gcgcttagcc ggtcgtcctt
    46261 gcgccgccgg gcgcgcacag tggagaatgt gaaaactccc gtttgcttct ctgaagttca
    46321 gttaaagcat cgacggccac gcccgcgggg ccggcaccga ggtgacgaat gcgcctgctg
    46381 cttcttacgc tgaccgttct gctcgcgtgc gccgcgtcgt cgcgcggctc cgggcgcgac
    46441 gactcaagcg cgccgcccga gccgggcgtc ggccgcgacc tcgcgcgcag tcgcgcgacg
    46501 cattaccgcg gcgtccgcta cgcgctcaac ctgacgctcc gtctcggcgc ggagttcctc
    46561 gacggcgaca tccgcatcag tctcacgctc gacaacccaa gcgaagacct cgtgctcgac
    46621 tggcgcgtgt tgaaaaagga cgcggaagcg cgcgggcgcg tctctgaaat cacggccaac
    46681 gaacgcgccg tggcggacgc gcgcttcgtg aacgatcata tcgtcatccc gtcggcatac
    46741 ctgcgcggcg gcgacaacgt cctgcggctc aagtttcgtt cgcccgtcag cgcctcgggc
    46801 agcgccgtca cgcgctacat cgaccgcgag gacaagtcgg aatacgtcta cacgctcttc
    46861 gtcccgtcgg acgcgagcac cgccttcccc tgcttcgatc agcccgacct caaggcgcgc
    46921 ttcacgctca ggctcacgac tcccgaaagc tggcgcgtcg tctcgaacac agactctcac
    46981 gtccaacccg aattcgatga gaccagcggc ggacacccgc agtcgcctcc cgccggaatg
    47041 ctctggcact ggttcgcgga gaccgagccg ctgagcacat accagttcgc cttcgcggcc
    47101 gggccgttcg tcgagttcaa ggagggcggc ggcgacgggc cggggaagca gacggccacg
    47161 ccgcagccat cggtgttgaa gaagggccgg cggagttcgt tgaaggcggt gagtgacgga
    47221 aacgcgtcga aggcgtcggg tggcgacgcg tttgggatgc gcgtcttcgt ccgcaa
//
LOCUS       DASVES010000321         8126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1375324, whole genome shotgun sequence.
ACCESSION   DASVES010000321 DASVES010000000
VERSION     DASVES010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375324"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VF240_13185"
     CDS             complement(<1..1215)
                     /locus_tag="VF240_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7176209.1"
                     /translation="MRLRLHDEAAPPRPLLALACCCAVILAACAPTFAQERPPRDVSR
                     EQARVVRPWVRDGVVYEIFPRSFSAEGNFEGVTRQLDRLRELGVNILWLMPIHPMGQE
                     KKKGSIGSPYAVRDFYAVNPDYGTKDDLKRLVAEAHRRGMKVFIDIVANHTSWDNVLM
                     KNPSYYVRDASGKINSPYDWTDVAELNYDDPALRRYMIDMLKFWVREFDLDGFRCDVA
                     GEVPTDFWEQARAELESIKPDIAMLAESDKPELLVKAFDFDYAWPMHGALNDVLHGRR
                     PASALRATWEADARKYPRGALRMRFSDNHDERRAIGRFGEPAALAASALMLTLDGVPM
                     LYNGMEAGDTTESGAPALFERLPIFWQTAERRPAFPRFYKQFLALRRSSAALRRGETE
                     WLKNSDESRVLTY"
     gene            complement(1212..2630)
                     /locus_tag="VF240_13190"
     CDS             complement(1212..2630)
                     /locus_tag="VF240_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176210.1"
                     /translation="MSRGLKLAAAACVVWLGLAAEARACGCVMSYGMYQPCGAYWSAD
                     VVFKGTVAEVGPLTPVPGSDGKVFTGAGQVTRFRVDEAFRGVAGDVVETSSEGTSCDY
                     HFKPGEQYFVYGSRSARDGKIYVSSCSATKTQERATADLAFARGAVRGEPTPSLIGLV
                     TREVREAASSYRSQRPLDGIEITIESGGKSAARARTDAEGAFRVFGLSPGVYAVRART
                     PRELRHLKVYGPETAQVRVLEGRCSAAAFVVTSLSTVSGRVVDATGAPTKTKVNLVPL
                     DESGAEVRPAEGTTEVYTDEGGRYLFEWVAPGRYRVAVNPHSQPGRSDPPYRRVYLPG
                     VFDPARSAVINVSDGGQHEAEDFRLPAPLTTGTVEGVVVTPDGKPVPHALLTLEFTER
                     EWIETERADEHGRFRLKLLEGYKYLVAAEVRSEVGGKWTGRHSEPAEVMAGEAGEPVR
                     LVITRPGFYAPLYVQRKRKEQK"
     gene            complement(2690..4168)
                     /locus_tag="VF240_13195"
     CDS             complement(2690..4168)
                     /locus_tag="VF240_13195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7176211.1"
                     /translation="MREKPRLSFWQIWNMSFGFMGIQFGWGLQLANMSGIYTYLGAKP
                     EEVPILWLAGPVTGLLVQPIIGSMSDRTWNRLGRRRPYFLVGAILASVALFFMPDSPT
                     LWMAAGMLWILDASINVSMEPFRAFVADKLNTDQRTAGFVMQSFFIGVGQTLANLLPY
                     LFRRAGVDERTAGAADAAVSAVPLSVNYAFKIGAVAFLVCVLWTVVTTREYPPEDMEE
                     FERRRSAYRGSGTNIFARIAAIIRELFAEIASAIRDMPATMKQLAFVQFFTWLGLFCM
                     WMFFGLMTSYHVFGARSERDPLFTDGQAWGGIAFMIYSIVCFIVAFLLAPMARATSRK
                     TVHAAALLCGAAGLFSVYFIQDKNILLLSMVGVGIAWASILSMPYAILSGALPAARMG
                     VYMGVFNFFIVIPEIIASFAFGPVIRAVFGPDNPRAPLFVVMAGGLFLLIAAACVALV
                     RDTSPREGAARDVIRGDEHEPLLTQQTAQPVPSTGLLDDDGR"
     gene            complement(4286..5011)
                     /locus_tag="VF240_13200"
     CDS             complement(4286..5011)
                     /locus_tag="VF240_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7176212.1"
                     /translation="MALRNKPLSEQVAVVTGGGTGIGRAFSEALAGAGARVIVASRRR
                     EVLEHAATEINRACGAELVFPYVFDVRERGECEALIDFAHGRFGAVDVLINNSGLAVP
                     ESVEEISGGGWDTVLETNVKGAMWLVRAALPRMIAEDFGDVVNVSSQAGKHGYADVPS
                     YCASKWALLGYAESLRDHVRKRGANVRVFNFCPGLVDVETTDANASPRPGFVHVSNMA
                     RTLLYALALDRHVVLEDINIYAR"
     gene            complement(5032..6969)
                     /locus_tag="VF240_13205"
     CDS             complement(5032..6969)
                     /locus_tag="VF240_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7176213.1"
                     /translation="MDNLKASFSRTVLIVLSVVVFAGVCVAQSAPLVEKVEPPSWWAN
                     HSINPVRLLVRGRNLDGMRLEAIAGGGMRTGLLRVNERGTYMFVDVFIESDAKPGRRS
                     LRFRNAGGAVDVPFELSAPLPRTGRYQGFTTDDVIYFIMPDRFADGDPSNNNPAKSPG
                     LYDRSKGRYYHGGDFQGIIDRLPYLKELGVTALWINPVYDNTDRLDERETYPEVEGGP
                     KRPTTAYHGYGAIDMYGVEEHYGTMSKLRELVERAHAAGIKVIQDQIANHTSPYHPWA
                     KERPTPSWFYGTEEKHLSNNWQKWTTMDPYATEATRRRNLQGWFIDILPDFNQDDEEV
                     RRYLIQNTLWWLGQVGFDAIRMDTLPHVPRHFWRDWSAAIKREYPNVNVLGELFDGDP
                     ALLAYFQRGRTGRDGIDPGIDTLYDFALHYAIRDAFAKGDSVRKLAQVLAHDHLYPNP
                     RVLVPFIGVHDMPRFMNEKGATTEGLKLAQTFVMTTRGTPLLYYGDELAMPGGGDPDN
                     RRDFPGGFPSDARNAFTKQGRTATENEVFNHLKTLARLRAQLPQLRRGALLHLYDEEQ
                     QTVFARTLDGYSPVIIAFNNDTKPASLDIDASSLGLPDGVKLRDWLGVVQEHFRPAGG
                     RIQLTLPARSAVVIDAQGAVQ"
     gene            complement(7057..>8126)
                     /gene="malQ"
                     /locus_tag="VF240_13210"
     CDS             complement(7057..>8126)
                     /gene="malQ"
                     /locus_tag="VF240_13210"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7176214.1"
                     /translation="LASARARLAEEIDEVKFKQFLFFRQWTRLRAYCREHGVRIIGDI
                     PIFVAHDSADVWAKPHLFKLDSAGRPRVIAGVPPDYFSRTGQLWGNPIYDWGRMVEDG
                     FAWWVERVRGMLSLVDIIRVDHFRGFAAYWEIPGGDQTAERGRWVYAPGRELFHAIKH
                     ELGTLPIIAEDLGFITPDVIELRDEFDFPGMRIFQFGFSSDTTNKDLPHNYVRNTVVY
                     TGTHDNDTAVGWFRSDPTLGASVRSAAQIERERRYCLDYLESDGTEINWDFIRAVFAS
                     AGDTALVPLQDLLGLGSEARMNTPATMSGNWAWRYRAADLSADLATRLRRLSELYGRH
                     PVLTDRPEHTAESYEAKQTWG"
BASE COUNT         1355 a         2610 c         2795 g         1366 t
ORIGIN      
        1 gtaagtgagg acgcgcgact cgtcggagtt tttcagccac tcggtctcgc cccggcgcag
       61 ggccgcggac gacctgcgga gcgcgaggaa ctgtttatag aagcgcggga aggcggggcg
      121 gcgctcggcc gtctgccaga agatgggcag gcgctcgaag agcgcgggcg cgcccgactc
      181 ggtcgtgtcg ccggcctcca tgccgttgta gagcatcggc acgccgtcga gcgtgagcat
      241 caacgcggag gcggcgagcg ccgccggctc gccgaagcgg ccgatggctc ggcgctcgtc
      301 gtggttgtct gagaagcgca tccgcaaggc cccacgcggg tacttgcgcg cgtccgcctc
      361 ccacgtcgca cgcagcgcgg aggcgggccg ccgcccgtgc agaacatcgt tgagcgcgcc
      421 gtgcatcggc cacgcgtaat cgaaatcgaa agccttcacc agcagctccg gcttgtccga
      481 ctcggcgagc atcgcaatat cgggcttgat gctttcgagt tcggcgcggg cctgctccca
      541 gaagtctgtg ggcacctcgc ccgccacgtc gcagcggaag ccgtcgaggt cgaactcgcg
      601 cacccagaac ttcagcatgt cgatcatgta gcgccggagc gcggggtcgt cgtagttcaa
      661 ctcggccacg tccgtccagt cgtaaggcga gttgatcttg ccggacgcat cgcgcacgta
      721 gtagctcggg ttcttcatca gcacgttgtc ccacgacgtg tgattggcga cgatgtcgat
      781 gaagaccttc atcccccggc ggtgcgcctc ggcgactaaa cgcttgaggt cgtccttcgt
      841 cccgtagtcg gggttgacgg cgtagaagtc gcgcacggcg taggggctgc cgatgcttcc
      901 cttcttcttc tcctgcccca tcgggtgaat cggcatcagc cagaggatgt tgacgccgag
      961 ttcgcgcagg cggtcgagct ggcgcgtgac gccctcgaaa tttccctcgg ccgagaaaga
     1021 gcgcgggaag atttcgtaga ccacgccgtc gcgcacccac ggccgcacca cgcgcgcctg
     1081 ctcccgcgac acgtcgcgcg gtgggcgctc ctgcgcgaac gtcggcgcgc aggcggcgag
     1141 gatgacggcg cagcagcagg ccagcgcgag gagtgggcgc gggggcgcgg cctcgtcatg
     1201 taggcgcagt ctcatttctg ctcctttcgc ttcctctgga cgtagagggg cgcgtagaag
     1261 ccggggcgcg tgatgacgag tctgaccggc tcgcctgcct ccccggccat gacctcggcc
     1321 ggctctgagt ggcgccccgt ccacttgccc cccacctcgg aacgcacctc ggctgcgacg
     1381 agatacttgt agccctctaa gagtttcaga cggaagcgac cgtgctcgtc ggcgcgctcg
     1441 gtctcaatcc attcgcgctc ggtgaattcg agcgtcagga gagcgtgcgg gacgggcttg
     1501 ccgtccggcg tcacgaccac gccctcgacc gtccccgtcg tgagcggggc gggcaggcgg
     1561 aagtcctccg cctcgtgttg gcccccgtcc gagacgttga tgacggccga gcgcgccggg
     1621 tcgaacacgc cgggcaggta gacgcgccgg tagggcgggt cggagcggcc gggctggctg
     1681 tgcgggttga cggccacgcg gtaacgcccc ggcgcgaccc actcgaacag atagcgcccg
     1741 ccctcgtcag tgtagacttc ggtcgttcct tcggccggac gcacctctgc tcccgactcg
     1801 tcgagcggga cgaggttgac tttcgtcttc gtcggcgcac cagtggcgtc cacgacccgg
     1861 ccgctgactg tcgaaagtga cgtgacgacg aaggccgcgg cgctgcaacg tccctccaag
     1921 actcgcacct gcgccgtctc ggggccgtag actttcaggt ggcgcaactc gcgcggcgtg
     1981 cgcgcgcgca cggcgtaaac ccccggcgag aggccgaaga cacggaaggc gccctcggca
     2041 tccgtcctcg cccgcgccgc cgacttgccc cccgactcga tggtgatctc gatgccgtcg
     2101 agcgggcgct gcgagcggta ggagcttgcc gcctcgcgca cctcgcgcgt gaccagaccg
     2161 atgagggagg gcgtcggctc gccgcgcacc gcgccgcggg cgaaggcgag gtcagccgtc
     2221 gcgcgctcct gcgtcttcgt cgcgctgcaa ctggagacgt agattttccc gtcgcgcgcg
     2281 ctgcgcgagc cgtagacgaa gtattgctcg ccaggcttga agtggtagtc gcagctcgtc
     2341 ccttcactcg acgtttcgac aacgtcgccg gcgacgccgc gaaacgcctc gtccacccgg
     2401 aagcgcgtga cctgtccagc ccccgtgaat accttcccgt cgctgcccgg cacgggcgtt
     2461 agcggcccca cctccgcaac cgtgcccttg aacaccacgt cggcgctcca gtaggccccg
     2521 cagggctggt acatcccata actcatcacg cacccgcacg cccgcgcctc ggccgccagc
     2581 ccgagccata cgacgcaagc cgcggccgcc aacttcagcc ccctgctcat agcactctcc
     2641 tttcccgtag gcgtcgcggc cccggcgccg ccggccgcat gaatcaacgt caccgcccgt
     2701 cgtcgtccaa caggccggtg ctcgggacgg gctgcgccgt ctgctgcgtg agcagcggct
     2761 cgtgctcatc gccgcggatg acatctcgcg ccgccccttc gcgcggcgac gtgtcgcgca
     2821 cgagcgcgac gcaggcggcg gcgatgagga ggaagaggcc gcccgccatc acgacgaaga
     2881 gcggagcgcg cgggttgtcc ggcccgaaga cggcgcggat gacggggccg aaggcgaacg
     2941 acgcgatgat ctccgggatg acgatgaaga agttgaagac gcccatgtag acgcccatgc
     3001 gcgcggctgg cagcgccccc gagagtatcg cgtagggcat cgagagaatc gacgcccagg
     3061 cgatgccgac gccgaccatc gacagcagca ggatgttttt gtcctggatg aagtagaccg
     3121 agaacagtcc ggctgcgccg cacaaaaggg cggcggcgtg gactgtcttg cggctcgtgg
     3181 cccgcgccat cggcgcgagc aggaaggcga cgatgaagca gacgatggag tagatcatga
     3241 aggcgatgcc gccccacgcc tgcccgtccg taaacagcgg gtcgcgctcg ctcctcgcgc
     3301 cgaagacgtg ataggaggtc atcagtccga agaacatcca catacagaac aggcccaacc
     3361 acgtaaagaa ctggacgaaa gcgagctgct tcatcgtcgc gggcatgtcg cggatggcgg
     3421 aggcgatttc cgcaaacaac tcgcggatga tggcggcgat gcgcgcgaag atgttcgtgc
     3481 cgctgccacg gtaggcgctt cgccggcgct cgaactcttc catgtcttcg ggcgggtact
     3541 cgcgcgtcgt cactaccgtc cagagaacgc agacgaggaa ggcgacggcg ccgatcttga
     3601 aggcgtagtt gacgctcagc gggacggcgc tcacggcggc gtcggccgcg cccgctgttc
     3661 gctcgtcaac accggcgcga cggaagagat agggcagaag gttggcgagc gtctgcccga
     3721 cgccgatgaa gaacgactgc atgacgaagc cggcggtgcg ctggtcggtg ttgagcttgt
     3781 cggcgacgaa ggcgcggaac ggctccatcg agacgttgat cgaggcgtcg agtatccaga
     3841 gcatcccggc ggccatccag agggtgggcg agtcgggcat gaagaagagg gcgacgctgg
     3901 cgaggatggc cccgacgagg aagtagggac ggcgtcgccc cagacggttc cacgtccggt
     3961 cgctcatcga gccgatgatg ggctggacga gcaggcccgt gacggggccg gcgagccaca
     4021 gtatcggcac ctcctcgggt ttcgcgccga ggtacgtgta gatgccgctc atgttggcga
     4081 gctgcaaccc ccagccgaac tggatgccca taaacccgaa gctcatgttc cagatttgcc
     4141 agaagctcag gcgcggtttc tctcgcatgg actctcctct cagtcagaga aggagtcgga
     4201 attcaggagt caggagccag aagaaaaagt gacggccgcc ctcttcattc tgactcctga
     4261 ctcctgaatt ctgactccct gcatttcatc tcgcatagat gttgatgtct tcaagcacga
     4321 cgtggcggtc gagcgcgagc gcgtaaagaa gcgtgcgcgc catgttggag acgtggacga
     4381 agccggggcg cggcgaggcg ttggcgtcgg tggtttccac gtccacgaga ccggggcaga
     4441 agttgaagac gcggacgttg gctccccgtt tgcggacgtg gtcgcgcaga gactcggcgt
     4501 agccgaggag cgcccacttc gaggcgcagt aggagggcac gtcggcgtag ccgtgcttgc
     4561 cggcctgcga ggagacgttg accacgtcgc cgaagtcttc ggcgatcatc cgcggcagcg
     4621 ccgcgcgcac gagccacatc gcgcccttca cgttcgtctc caaaaccgtg tcccaaccgc
     4681 cgccggaaat ctcctcgacc gactcgggga cggccagccc cgagttgtta atgagcacgt
     4741 ccaccgcccc gaagcgaccg tgcgcgaaat cgatgagcgc ctcgcactcg ccacgctcgc
     4801 gcacgtcgaa gacgtaggga aagaccagct cggctccaca cgcgcggttg atctcggtcg
     4861 ccgcatgctc caacacctcc cgccgccgcg aggcgacgat gacgcgcgcc ccggccccgg
     4921 ccagcgcctc actaaacgcg cgcccgatcc ccgtgccgcc gcccgtgacg acggccacct
     4981 gctccgacaa aggcttgttc ctgagcgcca cgttcccccc tcgcacgcgt gctactgcac
     5041 ggccccctgc gcgtcgatga cgaccgccga gcgggcgggc agcgtgagct gtatccgtcc
     5101 gccggcggga cggaagtgtt cctgaacgac cccgagccag tccctcagtt tgacgccatc
     5161 gggtagaccg agcgaactcg cgtcgatgtc gagggaggcc ggcttggtgt cgttgttgaa
     5221 ggcgatgatg accggcgagt agccgtcgag cgtgcgcgcg aagacggtct gttgctcctc
     5281 gtcgtagagg tgcagcagcg cgccgcgtcg gagttgcggg agttgggcgc gcaggcgggc
     5341 cagagtctta aggtggttga agacttcgtt ctcggtggcg gtgcgtccct gtttcgtgaa
     5401 agcgttgcgg gcgtccgagg ggaagccgcc ggggaagtcg cggcggttgt cggggtcgcc
     5461 gccgccgggc atggcgagtt cgtcgccata gtagaggagc ggtgtgccgc gcgtcgtcat
     5521 cacgaaagtt tgcgcgagct tgagtccttc ggtggtcgcg cccttttcgt tcatgaagcg
     5581 cggcatgtcg tggacgccga tgaaggggac gaggacgcgc gggttcgggt agaggtggtc
     5641 gtgcgcgagc acctgcgcga gcttgcgaac ggagtcgcct ttggcgaagg cgtcgcggat
     5701 ggcgtagtgg agcgcgaagt cgtagagggt gtcgatgccg gggtcgatgc cgtcccggcc
     5761 cgtgcggccg cgctggaagt aggcgagcag cgccgggtcg ccgtcaaaca actcgccgag
     5821 gacgttgacg tttggatatt cacgtttgat ggcggccgac cagtcgcgcc agaagtgtcg
     5881 ggggacgtgt gggagcgtgt ccatgcggat ggcgtcgaag ccgacctgac cgagccacca
     5941 gagcgtgttc tggatgagat agcggcgcac ttcttcgtcg tcctgattga agtcggggag
     6001 gatgtcgatg aaccaaccct gtaggttgcg ccggcgcgtg gcctcggtgg cgtaggggtc
     6061 catcgtcgtc cacttctgcc agttgttcga gagatgcttc tcctcggtgc cgtagaacca
     6121 agagggcgtc gggcgctcct tcgcccaggg gtgatagggg ctggtgtggt tggcgatctg
     6181 atcctgaatg accttgatgc cggcggcgtg ggcgcgctcg accagctcgc gcagcttcga
     6241 catcgtgccg tagtgttcct cgacgccgta catgtcgatg gcgccgtagc cgtggtaggc
     6301 ggtcgtcggc cgcttcgggc cgccctcgac ttccgggtac gtctcgcgct cgtcgagccg
     6361 gtcggtgttg tcgtagacgg gattgatcca cagggcggtg acgccgagtt ccttgagata
     6421 aggcaggcgg tcgatgatgc cctgaaagtc gccgccgtgg tagtagcgcc ccttcgagcg
     6481 gtcgtagagg ccgggcgact tggcggggtt gttgttcgac gggtcgccgt ccgcgaagcg
     6541 gtcgggcatg atgaagtaga tcacgtcgtc cgtcgtgaag ccctgatagc gacctgtgcg
     6601 cgggagcggc gcgctcagtt caaacggcac gtcaaccgcg ccgcccgcgt tgcggaagcg
     6661 gaggctacgg cggccgggct ttgcatccga ttcgatgaac acgtcaacga acatgtacgt
     6721 cccgcgctcg ttgactctca gaagccccgt cctcatccct cccccggcga tggcttcgag
     6781 acgcatgccg tcgaggttgc gcccgcgcac gagcaggcgc accgggttga ttgaatgatt
     6841 cgcccaccaa gacggcggct cgaccttctc gaccaacggc gcgctctgtg cgacacacac
     6901 accggcaaac acgacgaccg atagcacgat gagtaccgtt ctgctgaatg acgcctttaa
     6961 attatccatg attttccgtc tgactgatta gccgcgggtc acgcgccgga ctgcttttct
     7021 ctcatcactc atcactcatc actcatcact tcctattcat ccccacgtct gtttcgcctc
     7081 gtagctctcg gccgtgtgct cgggccggtc ggtgaggacg gggtggcggc cgtagagttc
     7141 ggagagacga cgtaagcgcg tcgcgaggtc ggcgctcagg tcggcggcgc ggtagcgcca
     7201 ggcccagttg ccggacatcg tggcgggcgt gttcatacgc gcttccgacc cgaggccgag
     7261 caagtcctgc aagggaacga gcgccgtgtc gccggccgag gcgaagacgg cgcggatgaa
     7321 gtcccagttg atctccgtgc catccgattc aaggtagtcg agacagtagc gtcgctcgcg
     7381 ctcgatctgc gcggcgctgc gcacggaggc gccgagcgtg gggtcgctgc ggaaccagcc
     7441 gacggccgtg tcgttgtcgt gtgtgccggt gtagacgacc gtgttgcgga cgtagttgtg
     7501 cggcaagtcc ttgttcgtcg tgtcggagct gaagccgaac tggaagatac gcatgccggg
     7561 aaagtcgaac tcgtcgcgca gctcgatcac gtccggggtg atgaagccga ggtcttcggc
     7621 gatgatgggg agcgtgccca gctcgtgctt gatggcgtgg aagagttcgc ggcccggcgc
     7681 gtagacccag cggccgcgct cggccgtctg gtcgccgccg ggtatctccc agtaggcggc
     7741 aaacccccgg aagtgatcga cgcggatgat gtccacaagc gagagcatcc cgcgcacgcg
     7801 ctcgacccac cacgcgaagc cgtcttcgac catccggccc cagtcgtaga tgggattgcc
     7861 ccagagctgc cccgtccggc tgaagtagtc gggcgggacg ccggcgataa cacggggccg
     7921 tcccgcgctg tccaacttga agaggtgggg cttggcccac acgtcggccg agtcgtgcgc
     7981 gacgaagatg ggaatgtcgc cgatgattcg cacgccgtgc tcccgacaat aggcgcgcag
     8041 gcgcgtccac tggcggaaga agaggaactg tttgaacttg acctcatcga tctcttcggc
     8101 gagacgcgcg cgcgccgacg cgagcg
//
LOCUS       DASVES010000322        19458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_80005, whole genome shotgun sequence.
ACCESSION   DASVES010000322 DASVES010000000
VERSION     DASVES010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80005"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..1623)
                     /locus_tag="VF240_13215"
     CDS             complement(304..1623)
                     /locus_tag="VF240_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7176215.1"
                     /translation="MRFTFTKLFYALLTLGFVPLSLSWSRPALRWLTVVFDAALLVAA
                     FIDARTSRLAEGVRVERETGGRFHIGAETEVRVRVTNQTGAAVSLRVKDEYPPELILR
                     GAREAHMRILPREVLTLVYGLTPPRRGRFEFGQVAVRYVSRLGLVWRETRAGRAETVK
                     VYPNIRRAREAELAALGARSLVAAHRRAAWRGEGRDFESLRDYVPGDELRHVSWTATA
                     RRGRLTTRQYQIERDQTILIALDSGRLMTGRVEGETKFDSAIHAALALMSAAERGGDQ
                     AGLAVFGRRVITYVPPGKGREQADAVLEALHPIEPQMIEPSYTRAFEYIAAHCRRRAL
                     VVVLTDLVDEEGSRDLLKSLSILRPRHLPLVVTIADRDLRSVVSEAPADVRGLFTQSV
                     AEEIIRQREAALRLVESRGGLALDVTTAALTPMLLETYVRVKERGLL"
     gene            complement(1726..2934)
                     /locus_tag="VF240_13220"
     CDS             complement(1726..2934)
                     /locus_tag="VF240_13220"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX7176216.1"
                     /translation="MPAVSATNAGGGEARLQRRLKITLAAAAACGLLLSWRLWVSSPR
                     QYPTVPVSRLLPPVPYPLDFVWLAALLALLAAVALARESRKFVVAFLVLAGLLCLWDQ
                     SRWQPWFYMYYFMLLALCIGLREGREPEDGAAALNTCRLIVAATYFWGGLQKVNYEFA
                     TLTVRSLLNPYLRHLPEELRPLTHVLAVVIPLAEICVGAGLLTRRFRDAAVVCACLMH
                     AFVLMLFVPVRNNVVIWPWNVAMAAVVVWLFWRDRSFSLREVLSGGRLFAHKLALALF
                     GVMPLLGLFGLWDSYLSAALYSGNTHYAAIYVSDSLRERLPPKARGVVRLARGRNMLN
                     IGNWSYAELNVPPYPEPRVYREAGRTVCRLAETPTDAVLEIHGRPNPLDGRRTIETWD
                     CDRLNTAISD"
     gene            complement(2969..4018)
                     /locus_tag="VF240_13225"
     CDS             complement(2969..4018)
                     /locus_tag="VF240_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX7176217.1"
                     /translation="MTAPFTFTLPEELSAKEPPERRGEGRDRVRLMVIDRGTHAVEHT
                     RFDHLGEYLREGDLLVFNSSRTLPAALEGCAAPAGPCIEMRLAERLPDDTWMALLMCQ
                     QGDPFACGLREGLEIVFGEGLRGKVLERDRRIPRLWRVRFSRTGAELIDTFYRVGRPI
                     RYEYVSAPWDLDYYQTVYAREPGSAEMPSAGRAFTWRLLFDLKRRGVRVAYVVLHTGL
                     SSYMDDELDAQHPASEEEYFITDAAAREINETRARGARVVAVGTTVVRALESVADEDG
                     VIRPGHGYTRLHVTAGHVLTVVTGLLTGLHEPEASHLDLLTAFLPAEEIRAAYQGAVE
                     LKYLWHEFGDLNLIV"
     gene            complement(4071..4301)
                     /locus_tag="VF240_13230"
     CDS             complement(4071..4301)
                     /locus_tag="VF240_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176218.1"
                     /translation="MKKDETEIQEDEMRPEYDLSQLKGRVRGKYVERYREGTNLVLLD
                     PDVAAAFPDAKAVNEALRLLIKVAQAPTSTPH"
     gene            complement(4282..4560)
                     /locus_tag="VF240_13235"
     CDS             complement(4282..4560)
                     /locus_tag="VF240_13235"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX7176219.1"
                     /translation="MLFQWDENKATGNLAKHGVSFEEAATIFGDSLSDTFNDPDHSAE
                     EQRFIIIGMSERGRLLAVVHTDEDEVVRIISARELTRREREVYEKGRN"
     gene            complement(4601..5134)
                     /locus_tag="VF240_13240"
     CDS             complement(4601..5134)
                     /locus_tag="VF240_13240"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7176220.1"
                     /translation="MSTTEQSAVTIRRATPADAALLAELGARTFNETFAADNRPEDMA
                     AYLASAFSVEQTERELSDPHSIWLVAEIDGDAAGYAKLRAGQAPECVRGSDPVELARI
                     YAARDWLGRGVGASLLRACLEEARRGGHRTIWLGVWERNERARAFYRKWDFRDTGTQI
                     FQLGSDTQTDVVMERAL"
     gene            complement(5161..5931)
                     /locus_tag="VF240_13245"
     CDS             complement(5161..5931)
                     /locus_tag="VF240_13245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7176221.1"
                     /translation="MNRLQGKHVLITGASQGLGRQLAIEYAREGAARLALAARRGEAL
                     EEVREAVREAAPEARVLNIVADLRRHEDVERTVATTLSEFGGRLDVLVNNASSIGPVP
                     LPFLLDYPLEDFRSVLDTNLVAPFILIKKTLPAMVERGGSIINVTSDAGVVGYPTWGA
                     YGISKFGLEGLSQTWAAELEGSGVRVNWVDPGDMNTEMHRQATPEEDPAQWAEPEEVT
                     EVFIYLASDESKHVNGRRFQAQEENWGQSEAPSEEQSD"
     gene            complement(5977..6927)
                     /locus_tag="VF240_13250"
     CDS             complement(5977..6927)
                     /locus_tag="VF240_13250"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7176222.1"
                     /translation="MQSVPSFVEHVRGELAKVIVGQEAVVEQVLVALLAEGHALVEGV
                     PGTAKTLLVKTLARIIGADFSRIQFTPDLMPSDITGTNVFNVATSNFTLRRGPVFTDI
                     LLADEINRTPPKTQAALLEAMEERQVTIDGELHHLSPVFTVLATENPIEYEGTYPLPE
                     AQLDRFLLKIIMGYPAAEEERRVVANWDEGFNARRLDAVDIRPLPDAAAVLHCRDEVR
                     ASRVEPGVRAYLVEIVRRTREHPSVAYGASPRASVAMLLCAKALAAMRDRPFATPDDV
                     RDVARPVMRHRLLLRAEAELEGATPDGVVEDILNTVEVPR"
     gene            complement(7016..7903)
                     /locus_tag="VF240_13255"
     CDS             complement(7016..7903)
                     /locus_tag="VF240_13255"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7176223.1"
                     /translation="MKKPESARARSIIPFAFLFFDSPLPLFLLLALFFATSVVGVVTG
                     SNSLITVPAMYQFGIDARVAVATNMFGLTFMSAGGALPFVGKGTFDRRRLPLLIALTL
                     AGSALGALLVLAVSSRAMPLFISVSMLAVAVFSVATRKRQVGRDAGFAGAGERGERAG
                     HSAGEGVSRRAEWAGYAATFALGVYGGFFSGGYVTLLTAAYVALFRVRFVEAVATTKV
                     VNVFSSLVATLIFMWAGLVDYRLGALLAVVMFAGAFVGARLALRVSERHLRAVFLATV
                     VVLALKILLYDFLWKGIQR"
     gene            complement(8081..9589)
                     /locus_tag="VF240_13260"
     CDS             complement(8081..9589)
                     /locus_tag="VF240_13260"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX7176224.1"
                     /translation="MQGRLAIFLTVAVVLVLLVALNAASYVRVETEAELEFRPDRSTH
                     NAGATGTRALHDFLRQSGFRVTRWAQAPSALRGGNVSERPETFVVVGQLRRPFEGDEA
                     RSLARWVWEGGRLVVIDRAPDEALLPASGGWRVSAETFDFPDMYTRPDEPEAMTAGVR
                     PVGPTQPTLLTRDVDQVMPSRFASRLHISTTANSGFGPAVPAASPRRTGDVKDGADES
                     DAARVEDDEEDATLSAPPPVLPPPSVGPVAGVETTDAPPEAATARAPVRHFPDARPGE
                     GALLVDYTYGRGRVVVLGDPYVVSNGGISRADNLQLAVNVVAGSRDGLIAFDEFHQGA
                     GGGRGRGAFAFFAGTPVLWMFAQAGAVALALLWSRGRRFARALPAPHVDRRSKLEFVA
                     SMAELQQRARAHGLAVENVYARTRRALARYAGLAPDAPHEEIAARVAARSGKDAAQLE
                     ALLAECEAAAAGSGQTPARKSLALVRHLRDLERDLGIRMRAREIRQREKVKR"
     gene            complement(9657..10061)
                     /locus_tag="VF240_13265"
     CDS             complement(9657..10061)
                     /locus_tag="VF240_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7176225.1"
                     /translation="MKFDRFEDIEAWKKARVLASDIYRITSSGEMGRDYGLRDQLRRA
                     SVSILSNIAEGFERGGNKEFYQFLALAKGSCGEVRAQLYVALDQHYVSDQQFNDISGR
                     ALEVSRIISGLMKYLKQSEMRGSKYKAEEFED"
     gene            complement(10126..11301)
                     /locus_tag="VF240_13270"
     CDS             complement(10126..11301)
                     /locus_tag="VF240_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX7176226.1"
                     /translation="MKVAREQNPSRPRVAVAFAATLAVAAWLGVIVAPAARAQANAQA
                     KRTTTAVADYRERVEQAASSLDALAVLSEEARSAAGGEEWAREEVSDILPGLRERTLR
                     ETRARVPPSERVERGDGGTLEVDNRWLHTELTRYERLPADDLRGEAGEEVVELAAERL
                     RALGERLKEFEEGAAAAGARRDKEAEKGRLAEILRGRDFAERGARGGALARIFESVVK
                     WLRDLLPDWQPLRPGVSEGASLVAQIVIYALSAGLLGYVAWRFWRRQRGGMPARARRR
                     EPRVVLGERLAPDETTTDLLAEAERLARAGDTRGAIRKAYVALLCELGERRVVRLAQN
                     KTNRDYLHAVREHAAHLYGLFHPLTLSFERHWYGVEDATETDWQNFRTECRRAIEAR"
     gene            complement(11298..12419)
                     /locus_tag="VF240_13275"
     CDS             complement(11298..12419)
                     /locus_tag="VF240_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176227.1"
                     /translation="MATLAVNESQLAPLSAGDLIDRTIRLYRSNFTPLVRASAPPVLV
                     TAVGGVLLTVAGRALGATESGGRVAIYFVLASLGVVLWAVGLLMHWVVMGGASRNLIM
                     HLLWGEPVSARTIYRHVRARFFGLLMASVLVGGWVLFCAGLAFFIWYVTLAVAALLAM
                     FASLGGVPVWIGAAVGLALFVAGTFAALFVFFWLFGRAAYVPQAMLVEGRGVFDAIAR
                     SVSLARQNARRLMAMFLFTTFAAYSASMLLLIPLGWVGYVSGVELFTLDQSEWPAWYA
                     VGYQVVWEVGTILLMPVWTLGLSLLYVDERVRHEGYDVELLAARVFGQMPDVPATTHT
                     PLSPAVAAAASAHETSAKVENPFVEERFRGSVLGLTDRR"
     gene            complement(12609..13235)
                     /locus_tag="VF240_13280"
     CDS             complement(12609..13235)
                     /locus_tag="VF240_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176228.1"
                     /translation="MWKLALLLLISGVSVAQQTPTPPPGSQGPEDVAVVKFSWRKERV
                     PGWENRQFSTSVESYDAMRARVDNERRIQQARNTGNKAELGRREDAARMLEDANSPKD
                     LKNTGPPRDGYRYKVIVSNAGTKTVKLIDWDYVFLDPNTRQEISRRQFTSEEKVRPGK
                     GKELEVFILSPPFLTVNAGAPRTGESPFVEQVILMRVEYSDGTIWQRP"
     gene            complement(13437..16709)
                     /locus_tag="VF240_13285"
     CDS             complement(13437..16709)
                     /locus_tag="VF240_13285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7176229.1"
                     /translation="MRGRLSFKSCLLVLVAFAFTLAVPTPSRAQLTRGAINGTVRDPA
                     GAAVSGATVRVFNPSTNISRDTTSDDDGFYRVAALEPGTYTVVIEGQGFSKVENRAVV
                     VQTSLETTFDANLQAGGVVETVDVTAQTEAITLNKTNATIGLTLTERQTEELPLSAAR
                     NVLNLALLAPNVHAAPGANGISANGQRARNNNFTIDGSDNNDITVTLSTTPVFPEAVG
                     EFQIQTNPYSAEYGRNSGAQINIITKSGTNEFHGDFFEFYRGSRLNALDNVEKRNGLT
                     RPSRFNRNQFGGTFGGPLHLPRFGEGDGPAYYSGRDRTHFFFGFQGDRTRSAGVLQAA
                     VTIPTAAGFSALQNIPLRAGQSAASRQAVLSQLNFLGAIYGQNPIYTNPQTVTINGTP
                     IQVGTTNLGVGQPNDTLTFIARADHRLGDNDNFTIRYITNQSQDANVVSNLTFGTLFS
                     GAQALFDQNLALSETHVFSPNVINEFRTSYIRRNLDFPENDTETLTVQIGGATCAAFC
                     FGGASNFPQSRVTNYFQFSDTMSWTTGRHGLKFGADIRFNQLFNKSGFDLKGTYTFNN
                     LADYLNNTAFSFAQAVSATNFLAEQYQQFYFVQDDWRVTPNLTLNLGLRYENATIPLG
                     FFGDPDPAHQAFSIPGPVQRDNNNFAPTFGFAWSPRPSEGSFLRRLLGDGESVIRGGY
                     RINYDLLFYNILVVNAGNFPFTAVNQVNNVVDQFPARVPVAAPTFNALNQFINTQVDA
                     RTPYSQLFSLTMQRELFRNYVLEFGYTGSRALDQIRQLQMNPSLLSEAQAAAVRARTA
                     IPSLQARRVNPAAGARVLIGPGAQSTYNAGFVALNRRLERGLQFGFSYTFGRNMSNND
                     ESLGVAAITNSSPQAPQDTFQINTEKSLSVFDRTHRLVVNYLYEAPAPWFARDNAFLR
                     QVFGGWQISGITSTQSGQPFTVFTGVDTNGNGSSVGDRPNFNPSATITPDPVTGDLRT
                     FTQDPTNPAFIVPRLNTAATAGPLIFTGTGAIQANSLGNGALGRNTFRGPGFWNTNLS
                     ISKNFRFREDMRVRISADFLNAFNQDNYGNPTSNMNSANFGRNLNNWGTRSITLGGKF
                     SF"
     gene            complement(16998..18341)
                     /locus_tag="VF240_13290"
     CDS             complement(16998..18341)
                     /locus_tag="VF240_13290"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX7176230.1"
                     /translation="MYEFAVTPAITQIRRRGVEITEVTPGSIASELELEAGDRVVRVN
                     GRLVRDYLDFRFQTGGETELTLNVLKGTGGEEWELEIERGEGEDLGLSFENIAPRQCA
                     NECLFCFCKGNPPDARPALFFRDEDVRLSFLYGNYTTLTSITEAEMRRVIEQRLTPQY
                     VSIHATDIDVRAYLLGVDKERADISDKLRRMMDAGIEIHAQVVVCPDINDGEVLRRTI
                     ADLASEHPRVRSVAIVPLGLTRYNTDERLTPVTPEWCRALVGEMATVQRELRARLGTT
                     FAFLGDEIYLRAGLSVPGRPHYGDYPQIEDGVGMVRSFREEFARLMRRLEKTPPRDPR
                     RLHGTIMSGALFAPVLSELINRLNERFGTRLRVAGVTNEYFGPEIVVAGLLTGRCVLA
                     ARDQIEGDFLILPDSAHKTGEPVMLDGTTLDDLGRSLGLPIHAVNLDGFGAMLLG"
     gene            18644..18997
                     /locus_tag="VF240_13295"
     CDS             18644..18997
                     /locus_tag="VF240_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176231.1"
                     /translation="MAANNPADIQEIIDSRLKRLEEQISALHKRVNDFRLINFILSGV
                     TLAGVVSFAFWLGGLNSELKRNTAELDKLNNTVKALSDKLIPPMAVVETKIENLDRKI
                     DRIDDKLTGQVAPQR"
BASE COUNT         3424 a         6158 c         6585 g         3291 t
ORIGIN      
        1 cccctacgtg atcggcatcg cgatgtggtc acactgcttc gaatgcaccg acgcgacagg
       61 aacctggaag tcgcatccga gcggcttccg gggcatgacc cactacctcg ccaacctcat
      121 caccaacaag cgcatcgcct atccgtagag ggctgaacgg cgcctcgctc gcatgcgcag
      181 gcacggggct ggcaaacatt ttaatttacc gcgcaatgct ccgcttgggg acatggctaa
      241 aaaacgccgt gcccctcaag ccgagcattg cgcgcttccg ccgcccgaca tctcaagtcc
      301 ctttcacaac agacctcgct ccttcacacg cacgtaggtt tcgagcagca tcggcgtcag
      361 cgccgccgtc gtcacatcga gggcgaggcc gccgcgcgac tcgacgaggc gcagggcggc
      421 ctcgcgctgg cggatgatct cctcagcgac cgactgcgtg aagaggccgc gcacgtcggc
      481 cggcgcttcc gagacgaccg agcgcaggtc gcggtcggcg atggtgacga cgagcggcag
      541 gtggcgcggg cgcaggatgg agagcgactt gagcaggtcg cgcgagcctt cctcgtccac
      601 aaggtctgtg agcacgacga cgagagcgcg ccgccggcag tgggcggcga tgtactcgaa
      661 ggcgcgggtg taagacggct cgatcatctg cggctcgata gggtggaggg cttcgagcac
      721 cgcatccgcc tgctcgcgcc ccttgccggg cgggacgtag gtgatgacgc ggcggccgaa
      781 gacggcgagg cccgcctgat cgccgccgcg ctcggccgcg ctcatcaggg cgagcgcggc
      841 gtggatcgcg gagtcgaact tcgtctcgcc ctcgacgcgg cctgtcatca gacgccccga
      901 gtctaaggcg atgaggatgg tctggtcgcg ctcgatctgg tactgccgtg tggtgagacg
      961 gccgcgccgg gccgtggccg tccaggagac gtggcgcagc tcgtcgccgg gcacgtagtc
     1021 gcgcagagac tcgaagtcgc gcccttcgcc gcgccacgcg gcgcgccggt gcgccgcgac
     1081 gagcgagcgg gcgccgagcg cggccagctc cgcctcccgc gcgcgccgga tgttcgggta
     1141 gaccttgacc gtctcggccc ggccggcgcg cgtctcgcgc cacaccagac cgaggcgcga
     1201 gacgtagcgc acagccacct gtccgaactc gaaccggccc cggcgcggcg gcgtcaggcc
     1261 atagacgagc gtcaacacct cgcgcggcag aatcctcatg tgcgcctcgc gtgcgcccct
     1321 caaaatcaac tccggcggat actcatcctt gacccgcagg ctcacggccg cgcccgtctg
     1381 attcgtgacg cgcacgcgca cctccgtctc cgcgccgatg tggaagcgcc cgcccgtttc
     1441 gcgctctacg cggacgccct cggcgagacg gctcgtgcgg gcgtcgatga aggccgcgac
     1501 gaggagggcc gcgtcgaaca ctaccgtcag ccagcgcagc gcgggacggc tccacgagag
     1561 cgagagcggc acgaagccga gcgtcaggag cgcgtagaag agtttggtga aagtgaatcg
     1621 catgtgtcag acagctatta gctgttagct ttcagcttaa agagagcgaa cgagaatcgt
     1681 gtttccgtgg caccgcgcct cacgatgtct gccgtgagct aagagctaat cgctaatcgc
     1741 cgtgttaagc cggtcgcagt cccacgtctc gatggtgcgg cgcccgtcga gcgggttggg
     1801 gcggccgtga atttcgagca ccgcgtccgt gggtgtttcg gccagacggc agaccgtgcg
     1861 ccccgcctcg cggtagaccc tcggctccgg gtacggcggg acgttcagct ccgcgtaaga
     1921 ccagttgccg atgttcagca tgtttcggcc cctcgccaga cgcactacgc cgcgggcctt
     1981 cggcggcagg cgctccctga gcgagtcgct cacgtagatg gccgcgtagt gtgtgttccc
     2041 ggagtagagc gccgccgaga ggtatgagtc ccagaggccg aagaggccga ggagcggcat
     2101 cacgccgaag agcgccagcg ccagcttgtg cgcgaacaga cggccgcccg agaggacttc
     2161 ccgcagcgag aaactgcggt cgcgccagaa gagccagacc accacggccg ccatcgccac
     2221 gttccacggc cagatgacga cgttgttgcg caccggcacg aagagcatca ggacgaaggc
     2281 gtgcatgagg cacgcgcaga cgacggccgc gtctctgaac ctgcgcgtca gcagccccgc
     2341 gccgacgcag atttcggcca gcgggatgac gacggctaac acgtgcgtga ggggacgcag
     2401 ctcttcgggc aggtgacgca ggtacggatt gaggagcgag cggacggtta gcgtggcgaa
     2461 ttcgtagttg accttctgga gccctcccca gaagtaagtc gcggcgacga tcaggcggca
     2521 cgtgttcagg gcggcggctc cgtcctccgg ctcgcggccc tcgcgcaagc cgatgcacag
     2581 ggccagaagc atgaagtagt acatgtagaa ccatggctgc cagcgcgact gatcccagag
     2641 gcacaacaag ccagcgagga cgagaaaggc gacgacgaac tttcgggact cgcgggcgag
     2701 ggcgaccgcc gcgagcagtg cgaggagcgc ggcgagccag acgaagtcga gcgggtaggg
     2761 gacgggcggc agcagccggc tcaccgggac ggtcgggtac tggcgcggcg aagagaccca
     2821 gagcctccac gaaagcagca gtccgcaggc ggcggccgcc gcgagcgtaa tcttcagccg
     2881 gcgctgaaga cgcgcctcgc ctcccccggc gttcgtggcg ctcacggctg gcatgttcgg
     2941 ggtagtcacc ggggcgttca aggatttgtc agacaattaa attcaggtcg ccgaactcgt
     3001 gccagaggta tttcaactcg acggccccct gataggcggc gcggatttct tcagcgggca
     3061 ggaaggccgt cagcaagtcg aggtggctcg cctccggctc gtgcagaccc gtgagcagcc
     3121 ccgtgacgac cgtcaggacg tggcctgccg tcacatgcag acgcgtgtag ccgtggccgg
     3181 ggcgaataac gccgtcttcg tccgcgacgg attcgagagc gcgcacgacc gtcgtcccga
     3241 cggcgacgac gcgcgctccc cgcgcgcgcg tctcgttgat ctcgcgcgcc gccgcgtccg
     3301 tgatgaaata ttcttcctcg gaggccgggt gctgcgcgtc cagctcgtcg tccatgtaag
     3361 acgacagccc cgtgtggagc acgacgtagg cgacgcgcac cccgcgccgc ttgagatcga
     3421 agagcagccg ccacgtgaag gcgcggcccg ccgagggcat ctcggccgag ccgggttcgc
     3481 gcgcgtagac ggtctggtag tagtccaaat cccacggcgc cgagacgtac tcgtaacgga
     3541 tgggacggcc cacgcgatag aacgtgtcga tcagctccgc ccccgtgcgc gagaagcgca
     3601 cgcgccacag acgagggatg cgccggtcgc gctccagaac cttcccccgc agcccttcgc
     3661 cgaagacgat ctccagcccc tcgcgcagcc cgcacgcgaa cgggtcgccc tgctgacaca
     3721 tcaacagcgc catccacgtg tcgtccggca gacgctcggc cagacgcatc tcgatgcacg
     3781 gccccgctgg ggccgcgcac ccttcgagcg cggccggcag cgtccgcgaa gagttgaaga
     3841 cgagcaggtc gccctcgcgc aggtactcac ccagatggtc gaagcgcgtg tgctcaacgg
     3901 cgtgcgtccc acggtcaatg accatcagac gcacgcggtc gcgcccctcg ccgcgccgct
     3961 cgggcggctc cttcgccgaa agctcttcgg gcagcgtaaa tgtgaagggt gcagtcatga
     4021 atccagtcac acccgcttca tcgattatcg gaggtggttg gatgtactac ttaatgcggg
     4081 gtagaagtcg gtgcctgggc aaccttaatc agtaggcgta aagcttcgtt caccgcttta
     4141 gcatccggga atgcagccgc cacatccggg tcaagcaaca ccagatttgt cccctcccgg
     4201 taacgctcaa catattttcc gcgcactcgt cccttcaatt gcgaaaggtc atactccggg
     4261 cgcatctcgt cttcctgtat ctcagtttcg tcctttttca taaacctctc tttctcggcg
     4321 agtgagttct cgcgcgctaa tgatccggac gacctcgtct tcatccgtgt gcacgacggc
     4381 taacagtcta cctctttcag acatgccaat gataatgaag cgttgttctt ctgcggagtg
     4441 gtccgggtca ttgaaagtgt cggacagcga atcaccgaaa atagtagcag cttcttcaaa
     4501 tgagactccg tgcttggcga ggttgcccgt ggctttattt tcgtcccatt gaaacaacat
     4561 gactcgcgct cctcataatc gacgcgccgc gaggcgaatt ctataacgcg cgttccatca
     4621 ccacgtcggt ctgcgtgtcg gagccgagtt gaaagatttg cgttcccgtg tcgcggaagt
     4681 cccacttgcg atagaaggcg cgggcgcgct cgttgcgctc ccagacgccg agccagatgg
     4741 tgcggtgtcc gccgcgccgc gcctcttcaa gacacgcgcg caggagcgac gcgcccaccc
     4801 cgcgccccag ccagtcgcgc gccgcgtaga ttcgcgccag ctcaacgggg tcggagccgc
     4861 gaacacattc gggcgcctgt cccgcgcgga gtttggcgta gccggctgcg tcgccgtcga
     4921 tttcggcgac gagccagatt gagtgggggt cggacagctc gcgctcagtc tgctcgacgc
     4981 tgaaggcgga ggcgaggtag gcggccatgt cttcgggccg gttgtcggcg gcaaaggttt
     5041 cgttgaaggt gcgcgcgccc agctccgcga gcagcgcggc gtcagcgggc gtggcgcggc
     5101 gaatggtgac ggcggattgt tcggtcgtgc tcatcgaaat tcttgatgca cagtgcttcg
     5161 tcagtccgac tgctcctcgc tcggggcttc ggactgtccc cagttttcct cctgcgcctg
     5221 aaagcgtcgg ccgttgacgt gcttcgactc gtcggaggcg aggtagatga agacttcggt
     5281 gacttcctcg ggttcggccc actgcgcggg gtcttcttcg ggcgtggcct gccggtgcat
     5341 ctctgtgttc atgtcgccgg ggtctaccca gttgacgcgc acgccgctgc cctcgagttc
     5401 ggccgcccac gtctgcgaca gtccttcgag tccgaacttg gagatgccgt aagcgcccca
     5461 cgtcgggtag ccgaccactc cggcgtcgct cgtcacgttg atgatcgagc cgccccgttc
     5521 gaccatcgcc ggcagcgtct tcttgatgag gatgaagggc gcgacgaggt tcgtgtcgag
     5581 gacgctgcgg aagtcttcga gcgggtagtc gagcaggaag ggcaagggca cggggccgat
     5641 gctcgaagcg ttgttgacga gcacgtcgag gcgtccgccg aactcactga gcgtggtggc
     5701 gaccgtgcgt tccacgtcct catgccggcg caggtcggcg acgatgttga gcacgcgcgc
     5761 ctcgggcgcg gcctcgcgca ccgcctcacg cacctcttcc aacgcctcgc ccctccgcgc
     5821 cgccagcgcc agccgggccg cgccctcgcg cgcgtactca atcgccaact gtcgccccag
     5881 cccctgcgac gcgcccgtga ttaaaacgtg cttgccttgc agcctgttca cgtcgagtat
     5941 cctccggcat gaaagtctcg gatgattata gacctctcaa cgcggcactt cgaccgtgtt
     6001 gagaatgtct tcgacgacgc cgtcgggcgt ggcgccttca agctcggcct cggcgcgcag
     6061 cagaagacga tggcgcatga cgggacgggc cacgtcgcgc acgtcgtcgg gggtggcgaa
     6121 ggggcggtcg cgcatggcgg caagggcctt cgcgcacaaa agcatcgcga ccgaggcgcg
     6181 ggggctggcg ccgtaggcga cgctggggtg ctcgcgcgtg cggcggacga tctcgacgag
     6241 gtaggcgcgc acgcccggct cgacgcgcga ggcgcgcacc tcgtcgcggc aatgcaggac
     6301 ggccgccgca tcggggagcg ggcggatgtc tacggcgtcg aggcggcgcg cgttgaagcc
     6361 ttcgtcccag ttggcgacga cccggcgctc ctcttcggcc gcggggtagc ccatgatgat
     6421 cttgagcagg aagcggtcga gctgcgcctc ggggagcggg tacgtgccct catactcgat
     6481 ggggttctcg gtggcgagaa cggtgaagac gggcgagagg tggtgcaact cgccgtcgat
     6541 ggtcacctgc cgctcctcca tcgcttcgag cagggcggcc tgcgtcttgg gcggcgtgcg
     6601 gttgatctcg tcggcgagca ggatgtcggt aaagacgggg ccgcgccgca gcgtgaagtt
     6661 cgaggtggcg acgttgaaga cgttcgtgcc cgtgatgtca gaaggcatca ggtcgggcgt
     6721 gaactggatg cggctgaagt cggccccgat gatgcgggcg agggtcttga cgaggagcgt
     6781 cttggccgtg ccgggcacgc cctcgacgag cgcgtggccc tcggcgagca gcgccaccaa
     6841 cacctgctcg acgaccgcct cctgcccgac gatgaccttc gccaactccc cgcgcacgtg
     6901 ctcgacgaat gagggaacgc tttgcatcgc ttgaggagtg ataagtgatg agtgataagt
     6961 gataagaaag aacagacggg ctttgtcttt cttatcactt atcactcatc acttatcacc
     7021 gttgaatccc tttccagaga aaatcgtaga gcaagatttt gagcgcgagg acgacgacgg
     7081 tggcgaggaa gacggcgcgc aggtgtcgct cgctcacgcg gagcgcgagg cgcgcgccga
     7141 cgaacgcgcc ggcgaacatg acgacggcga ggagcgcgcc gagccggtaa tcgaccagcc
     7201 ccgcccacat gaaaataagt gtggcgacga gcgacgagaa gacgttgacg accttcgtcg
     7261 tcgccacggc ctcgacgaag cgcacgcgga agagcgcgac gtaggcggcc gtcagcagtg
     7321 taacgtaccc gccgctgaaa aacccgccat agacgccgag cgcgaacgtc gccgcgtagc
     7381 ccgcccactc ggcgcggcgg gagacgccct cgcccgccga atgtccggcg cgctcgcccc
     7441 tctcacctgc cccggcgaac ccggcgtcgc gcccgacctg ccgcttgcgc gtggcgacgg
     7501 agaagacggc cacggcgagc atcgagacgg agatgaagag cggcatcgcg cgcgaggaga
     7561 cggcgagcac gaggagcgcg ccgagggctg agccggcgag cgtgagcgcg atgagcagcg
     7621 gcaggcggcg gcggtcgaac gtccccttgc cgacgaaggg cagcgcgccg cccgcgctca
     7681 tgaaggtgag gccgaacatg tttgtcgcca ccgccacacg cgcgtcgatg ccgaactggt
     7741 acatggccgg cacggtgatg agtgagttgc tgcccgtcac cacgccgacc acgctcgttg
     7801 cgaagaagag agcgaggagt aagaagaggg gaagcgggga atcaaaaaag agaaaggcga
     7861 aggggatgat actgcgcgcc cgtgctgact cgggtttctt cacctttgct cctgtccgat
     7921 gtgtgatgga aggcgactca ccgcggaggc gcaaagggcg cggagttcac gccgcaggct
     7981 tgctgtttct tctccgcgcc ccctctgcgt tctctgcggt gagtccaaca ttcgacatac
     8041 tcaacttaca aacgtcggac gcccgctctt cgtctaacca tcaccttttg accttctccc
     8101 tctgcctgat ctcgcgcgcg cgcatgcgta tgccgaggtc gcgctctaaa tctcgcaagt
     8161 gccgcacgag cgcgagcgac ttgcgcgccg gggtctgccc actgcccgca gccgccgcct
     8221 cacactcggc gaggagcgcc tccaactgcg ccgcgtcttt gccggagcgc gcggcgactc
     8281 gcgcggcgat ctcttcgtgc ggagcgtcgg gcgcaagtcc cgcgtagcgc gccagcgccc
     8341 ggcgcgtgcg cgcgtagacg ttctcgacgg cgaggccatg cgcgcgcgcc cgctgctgga
     8401 gttcggccat cgaggcgacg aactccagct tcgagcggcg gtcaacgtgc ggcgcgggga
     8461 gagcgcgcgc gaacctgcgc ccacgcgacc acagcagcgc cagcgcgacg gcccccgcct
     8521 gcgcgaacat ccagagcacg ggcgtccccg cgaagaaggc gaaagcgccg cgcccgcgcc
     8581 ccccgcccgc gccctgatgg aactcgtcga acgcaatgag gccgtcgcgg ctcccggcga
     8641 cgacgttgac ggcgagctgc aagttgtccg cgcgactgat gccgccattg gagacgacgt
     8701 aggggtcgcc gagaacgacg acgcgcccgc gcccgtaagt gtaatcgacg agcagcgcgc
     8761 cctcgccggg gcgtgcgtcg gggaagtgtc ggacgggcgc gcgcgccgtg gctgcttccg
     8821 gcggcgcgtc cgtcgtctcc actcccgcga ccggccccac cgaaggcggc ggcagaaccg
     8881 gcgggggagc cgacaaggtc gcatcttctt cgtcgtcttc gacccgcgcc gcatcgcttt
     8941 catcagcgcc gtctttaacg tcgcccgtgc ggcgtggcga ggcggcgggg acggcggggc
     9001 cgaagccgct gttcgcggtc gtactgatgt ggaggcgcga ggcgaagcgt gagggcatga
     9061 cctgatccac gtcgcgcgtg agcagcgtgg gctgcgtcgg gccgacgggg cgcactccgg
     9121 ccgtcatcgc ctccggctcg tcgggccgcg tgtacatatc tggaaagtcg aaggtctcgg
     9181 cggagacgcg ccagccgccg gaagcgggca gcagcgcctc gtcaggcgcg cggtcgatga
     9241 cgacgaggcg gcccccctcc cagacccagc gcgcgagcga gcgcgcctca tcgccctcga
     9301 agggccggcg caactgcccg acgacgacga acgtctcggg ccgctccgag acgttaccgc
     9361 cccggagggc cgacggggct tgcgcccagc gcgtcacgcg gaagcccgac tgtcggagga
     9421 aatcatggag ggcgcgcgtc cccgtcgccc cggcgttatg cgtcgagcgg tcgggccgaa
     9481 actccagctc ggcctcggtc tcgacgcgca cgtaggaagc cgcgttgagc gccacgagga
     9541 gcaccagtac gaccgccacg gtcaggaaga ttgcgagacg cccttgcaca gatatagttc
     9601 aaagttcaaa gttcaaagtt caaagtcagg agagcggatg ctcctgactt cttgcttcaa
     9661 tcttcaaact cttctgcttt gtatttagac ccacgcattt cagactgctt caggtacttc
     9721 atcaggccgg agatgatgcg gcttacttca agggcgcggc ctgagatgtc gttaaattgc
     9781 tgatcgctca cgtaatgttg atccaaagcg acataaagct gtgccctgac ttccccgcat
     9841 gatcccttgg ccaacgctaa gaactgatag aactctttat ttccgccgcg ctcgaatcct
     9901 tcagcaatgt tcgacagaat tgacactgaa gcgcggcgca gttgatcccg taaaccgtag
     9961 tcgcgcccca tctctccact ggacgtaatt cggtaaatat cactcgccag cacgcgcgcc
    10021 ttcttccaag cctcaatatc ctcgaaccta tcaaacttca atttacttct cctaaaccga
    10081 agtctctaaa tctccccttt gaactttgaa ctttgaactt tgaactcatc ttgcttcgat
    10141 ggcgcggcga cactccgtgc ggaagttctg ccagtccgtt tcggtcgcgt cctcgacgcc
    10201 gtaccagtgg cgctcgaatg acagagtgag cggatggaag aggccgtaga ggtgtgcggc
    10261 gtgctcgcgc acggcgtgaa gatagtcgcg gttggtcttg ttctgcgcga ggcgaacgac
    10321 gcggcgctcg cccagctcgc agagcagcgc gacgtaagcc ttgcggatgg ccccgcgcgt
    10381 gtcgccggct cgggcgagac gctcggcctc ggcgagaagg tcggtcgtcg tctcgtcggg
    10441 cgcgaggcgc tcgcctaaaa cgacacgcgg ctcacgccgg cgcgcgcgcg cgggcatgcc
    10501 cccgcgttgc cgacgccaga atctccacgc gacgtaaccg agaaggccgg ccgacagcgc
    10561 gtagatgact atctgcgcga ccaacgaggc gccctcgctc acaccgggcc ggagcggctg
    10621 ccagtcgggg agcaggtcgc gtagccactt cacgacgctt tcaaagatgc gcgcgagtgc
    10681 gcccccgcgc gcgccgcgct cggcgaagtc gcggccgcgc aggatttccg ccagccgccc
    10741 cttctccgcc tccttgtcgc ggcgcgcgcc cgccgccgcc gccccctcct caaactcctt
    10801 caggcgctcg cccagcgcgc gcaggcgctc ggccgccagc tccacaacct cctcgccagc
    10861 ctcgccccgc aagtcgtcgg ccggcagacg ctcgtagcgc gtaagctctg tgtggagcca
    10921 gcggttgtcc acttcgagcg tgccgccgtc gccgcgctcc acacgctccg atgggggcac
    10981 gcgcgcgcgc gtctcgcgca acgtccgctc ccgcagaccc ggcagaatgt cactcacctc
    11041 ttcgcgcgcc cactcctcgc cgcccgcggc gctgcgcgcc tcctccgaca acacggcgag
    11101 cgcgtcgagc gacgaagccg cctgctcgac gcgctcgcgg taatcggcga ccgcagtcgt
    11161 cgtcctcttc gcctgcgcgt tcgcctgcgc gcgcgcggcc ggggcgacga tgacgccgag
    11221 ccacgccgct acggcgagcg tcgcggcgaa ggcaacggcg acacgcggcc ttgaaggatt
    11281 ttgctcgcgc gcgactttca tcgtctgtcc gtcagaccga gcacggagcc tcggaaacgc
    11341 tcctcgacga aagggttctc caccttcgca ctcgtctcgt gagccgaggc ggcagcggcg
    11401 acggccggcg agagcggcgt gtgcgtggtg gccggcacgt cgggcatctg cccgaagacg
    11461 cgcgcggcga ggagttccac gtcgtagccc tcgtggcgca cgcgctcgtc cacgtagagc
    11521 agcgagaggc cgagcgtcca gaccggcatc agaaggatcg tgcccacctc ccagacgacc
    11581 tgatagccga cggcgtacca cgccggccac tcgctctggt cgagcgtgaa gagttccacc
    11641 ccgctcacgt agccgaccca cccgagcggg atgagcaaaa gcatcgacgc cgagtaggcg
    11701 gcgaaggtcg tgaagaggaa catcgccatc agacgccggg cgttctggcg cgcgagtgag
    11761 acgctgcggg cgatggcgtc gaagacgccg cgtccctcga cgagcatcgc ctgcggcacg
    11821 taggccgcgc gcccgaacag ccagaagaag acgaagagcg ccgcgaacgt ccccgccacg
    11881 aagagcgcga gtccgaccgc cgcgcctatc cagaccggca ccccgccgag actcgcgaac
    11941 atcgccagca gcgcggcgac cgcaagggtc acgtaccaga tgaagaaggc cagccccgca
    12001 cagaacagca cccagccgcc gaccagcacg gaagccatga gcaggccgaa gaagcgcgcg
    12061 cgcacgtggc gatagatggt acgcgccgag accggctcgc cccacagcag gtgcatgatg
    12121 aggttgcgcg acgcgccgcc catcaccacc cagtgcatca gtagccccac ggcccacagc
    12181 acgacgccca gcgacgccag cacgaaatag atggcgaccc ggccgccgct ctcggtcgcc
    12241 ccgagggcgc gacccgccac ggtcaaaagc accccgccga cggccgtcac caaaacgggt
    12301 ggggccgagg cgcgaacgag tggcgtgaag ttgcttcggt agagtcggat ggtgcggtcg
    12361 atgaggtcgc ccgcgctgag cggcgccagt tgcgattcgt tgactgcgag cgtcgccatc
    12421 aggtgcggct ttcagagata acgtgggagg tggcccgagc acgtccgaac gtcgggcggg
    12481 gccgaagtgt agcacacgcc ccgcgggccg actcgcgccg accgcgcgcg ccgaaagcgg
    12541 aagggcgact caactaaagt ttgaagcgcc ctttcaactc cacgccggca aggcaaaggt
    12601 cattcggttc aggggcgttg ccagatggtg ccgtccgaat attcgacgcg catcaggatc
    12661 acctgctcga cgaagggaga ttcgcccgtc cgcggcgctc ccgcgttgac ggtcaggaag
    12721 ggcggcgaaa ggatgaacac ttccagctct ttacctttgc cgggccgcac cttttcctcg
    12781 ctggtgaact ggcggcggga gatttcctgc cgcgtgttcg ggtcgaggaa aacgtagtcc
    12841 cagtcaatga gtttgacggt cttcgtgccg gcgttactga cgatcacctt gtacctgtag
    12901 ccgtcgcgcg gcggcccggt gtttttcaaa tctttggggg agttggcgtc ttcgagcatc
    12961 ctcgcggcgt cctcgcgccg gccgagttcc gccttgtttc cggtgttgcg cgcctgctga
    13021 atgcgcctct cgttatcgac ccgcgcgcgc atcgcgtcgt agctctcgac ggaggtgctg
    13081 aactgcctgt tctcccagcc gggcacgcgc tccttccgcc aactgaactt gacgacggcg
    13141 acatcctccg gcccttgaga gccgggcggc ggcgtcggcg tctgttgcgc gacgctcacc
    13201 cccgaaatta aaagcagcaa tgccagcttc cacatcgcct tcctcccttc cggccccgaa
    13261 gcgccggggc cggcattata ctccaaccga aaatttatgt gtcgccccgc cgccggccgc
    13321 aagggccgca cggtttcgcg gcgccggata aagaaaaggc gccccctgcg gagggggtgc
    13381 cttttgaagt ggcccgaagg ccgaacgaag taaggggcgg agaggccgta cttactttag
    13441 aagctgaact tgccgcccag cgtgatgctc cgggtgcccc agttgttgag gttgcgaccg
    13501 aagttggcgc tgttcatgtt gctggtcgga ttgccgtagt tgtcctgatt gaaggcgttc
    13561 aggaagtcgg cgctgatgcg gacgcgcatg tcctcgcgga acctgaagtt cttcgagatg
    13621 ctcaggttgg tgttccagaa gccggggccg cggaaggtgt tccggccgag ggcgccgttg
    13681 ccgagcgagt tggcctgaat ggcgcccgtc cccgtgaaga tcagcgggcc ggccgtggcg
    13741 gccgtgttga ggcgcgggac gatgaacgcc gggttggtcg ggtcctgtgt gaaggtgcgg
    13801 aggtcgccgg tcacggggtc gggggtgatc gtcgcggacg ggttgaagtt cggacggtcg
    13861 ccgacggaac tgccgttgcc gttcgtgtcc acgccggtga agacggtgaa gggctggccc
    13921 gactgggtcg aggtgatgcc ggagatctgc cagccgccga agacctgacg gaggaaggcg
    13981 ttgtctctgg caaaccacgg cgccggcgcc tcgtagagat agttgaccac gaggcggtgg
    14041 gtgcggtcga agaccgacag gctcttctcc gtgttgatct ggaaggtgtc ctgcggagcc
    14101 tgcggcgacg agttggtgat ggcggccacg ccgagcgact cgtcgttgtt gctcatgttg
    14161 cggccgaacg tgtaggagaa gccgaactgt aggccgcgct caaggcggcg gttgagcgcg
    14221 acgaagcccg cgttgtaggt ggactgggcg ccggggccga tgagcacgcg ggcgccggcg
    14281 gccgggttga cgcgccgggc ctgcaagctg ggaatcgccg tgcgggcgcg caccgcggcg
    14341 gcctgcgcct cgctcagcag cgacgggttc atctgaagct gtctgatctg atccagcgcg
    14401 cggctgcccg tgtagccgaa ttcgagcacg tagttgcgga acaactcgcg ctgcatggtg
    14461 aggctgaaga gctgtgagta aggcgtccgg gcatcgacct gcgtgttgat gaactggttg
    14521 agcgcgttga aggtcggcgc ggccaccggc acgcgggccg ggaattgatc gaccacgttg
    14581 ttgacctgat tgacggcggt gaacgggaag ttgccggcgt tgacgaccag aatgttgtag
    14641 aacagcaggt cgtagttgat ccggtagccg ccgcggatga ccgactcgcc gtcgcccaag
    14701 agcctccgca ggaagctgcc ctcagacggg cgcgggctcc aggcgaagcc gaaggtgggc
    14761 gcgaagttgt tgttgtcgcg ctgcacgggg ccggggatgg agaacgcctg atgcgccggg
    14821 tcggggtcgc cgaagaagcc gagcgggatg gtcgcgttct cgtagcggag gccgaggttg
    14881 agggtcaggt tcggggtcac gcgccagtcg tcctgcacga agtagaactg ctggtactgc
    14941 tcggcgagga agttggtcgc cgagacggcc tgcgcgaacg agaaggcggt gttgttgagg
    15001 tagtcggcca gattgttgaa ggtgtaggtg cccttcaggt cgaacccgga cttgttgaag
    15061 agctggttga agcggatgtc ggccccgaac ttcagcccgt ggcggccggt cgtccagctc
    15121 atcgtgtcgg agaactggaa gtagttggtg acgcggcttt gcgggaagtt gctggcgccg
    15181 ccgaagcaga aggccgcgca ggtggcgccg ccgatctgga cggtgagcgt ctccgtgtcg
    15241 ttctcaggga agtcgaggtt gcggcggatg taggaggtgc gaaactcgtt gatgacgttc
    15301 gggctgaaga cgtgcgtctc gctgagcgcc agattctggt cgaagagcgc ctgagcgccg
    15361 gagaagaggg tgccgaaggt cagattgctc accacgttgg cgtcctgcga ctggttggtg
    15421 atgtagcgga tcgtgaagtt gtcgttatcg ccgagccggt gatcggcgcg ggcgatgaag
    15481 gtcagggtgt cgttcggctg gcccacgccg aggttggtcg tgccgacctg gatgggagtc
    15541 ccgttgatgg tcacggtctg ggggttggtg tagatcggat tctgcccgta gatggcgccc
    15601 agaaagttga gctgactgag cacggcctga cggctggccg ccgactggcc ggcgcgcagc
    15661 ggaatgttct ggagcgccga gaaccccgcc gcggtcggga tcgtgacggc ggcctgaagc
    15721 acgccggccg agcgcgtgcg gtcgccctgg aagccgaaga agaagtgtgt gcggtcgcga
    15781 cctgagtagt aggccgggcc gtcgccctcg ccaaagcgcg gcaggtggag cgggccgccg
    15841 aaggtgccgc cgaactggtt gcggttgaag cgagagggcc gggtcaggcc gttgcgcttc
    15901 tcgacgttgt cgagcgcgtt gaggcggctg ccgcggtaga actcaaagaa gtcgccgtga
    15961 aattcgttgg tcccggactt cgtgatgatg ttgatctgcg cgccggagtt gcgcccgtac
    16021 tcggcgctgt aggggttggt ctgaatctgg aactcgccca ccgcctcggg gaagaccggg
    16081 gtggtcgaga gcgtgacggt gatgtcgttg ttgtccgagc cgtcgatggt gaagttgttg
    16141 ttgcgcgcgc gctggccgtt ggccgagatg ccgttggcgc cgggcgcggc gtggacgttc
    16201 ggggcaagca gcgcgaggtt gaggacgttg cgcgcggccg acaggggcaa ctcctccgtc
    16261 tgccgctcgg tcagcgtcag gccgatggtc gcgttcgtct tgttgagcgt gatggcctcg
    16321 gtctgggcgg tcacgtcaac cgtctcgacg accccgccgg cttggaggtt ggcgtcgaag
    16381 gtcgtctcca gcgaggtctg cacgacgacg gcgcggttct cgactttgga gaacccctga
    16441 ccctcgatga cgaccgtgta agttcccggc tcaagggcgg cgacgcggta gaacccgtcg
    16501 tcgtcggagg tcgtgtctct ggaaatgttc gtcgagggat tgaagacgcg aacggtcgcg
    16561 ccggagacag ccgcgccggc cgggtcgcgg acagtgccgt taatcgcacc gcgcgtcaac
    16621 tgcgcccgcg atggcgtcgg cactgccaac gtaaaggcga aggccactag caccagcagg
    16681 caagatttaa agctcaaacg tcctctcatc tttccctcct ctagcatagg cattagcggt
    16741 tgagtggtag gcgacggaaa caaattgagc gaggcttgaa gacttccatt acccggctga
    16801 caaaacgctt cgtaaaacgg ctacggcaaa gaatatgcca agatgggaac gaaaagcaga
    16861 cggccgtttt cctcatatat ttggccatta gtggacaagg gacagcgagg gtgtccatat
    16921 tttcaggaga ccgatacatt tttttggatt tgggctgttg aggccgggcg gggaagtttc
    16981 tctggtattt cccggtctta cccgagcaac atggcgccga agccgtcgag gttcacggca
    17041 tggatgggga ggccgaggct gcgccccagg tcgtcgagcg tcgtgccgtc gagcatgaca
    17101 ggctcgcccg tcttgtgggc cgagtccggg aggatgagga agtcgccctc tatctggtcg
    17161 cgcgcggcga ggacgcagcg gcccgtgagc aggccggcga cgacgatctc ggggccgaag
    17221 tattcgttcg tgacgccggc cacgcgcagg cgcgtgccga agcgctcgtt gagacgattg
    17281 ataagctcgg agaggacggg ggcgaagagc gcgcccgaca tgatcgtgcc gtggaggcgg
    17341 cgagggtcgc gcggcggcgt cttctccagc cggcgcatga ggcgcgcgaa ctcctcccga
    17401 aaggagcgca ccatgccgac gccgtcttcg atctgcggat agtcgccgta gtgcgggcgg
    17461 ccgggcacgg agagtccggc gcgcagatag atttcgtcgc cgaggaaggc gaaggtcgtc
    17521 cccagacgcg cgcgcagctc gcgctgcacg gtcgccatct cgccgacgag cgcgcggcac
    17581 cactcagggg tgacgggcgt gagccgctcg tccgtgttgt agcgcgtcag gcccaacggg
    17641 acgatggcga ccgagcggac gcgcgggtgc tccgaggcga ggtcggcgat ggtgcgccgc
    17701 aggacttcgc cgtcgttgat gtcggggcag acgacgacct gcgcgtgaat ctcgatgccg
    17761 gcgtccatca tccggcggag cttgtcggag atgtcggcgc gctctttatc cacgccgagc
    17821 aagtaggcgc gcacgtcgat gtcggtcgcg tggatggaga cgtattgcgg cgtgaggcgt
    17881 tgctcgatga cgcggcgcat ctctgcttcg gtgatcgagg tgagcgtggt gtagttgccg
    17941 tagaggaagg agaggcgcac gtcctcgtcg cggaagaaga gggcggggcg cgcgtcgggc
    18001 gggttgccct tgcagaagca gaacaggcac tcgttggcgc actggcgcgg ggcgatgttc
    18061 tcgaacgaaa ggccgaggtc ttcgccctcg ccgcgctcga tctccagctc ccactcctcg
    18121 ccccccgtgc ccttgagcac gtttaaggtg agttccgtct cgccgcccgt ctgaaaacgg
    18181 aagtcgaggt aatcgcgcac gaggcggccg ttgacgcgca cgacgcggtc gccggcctcc
    18241 agctccagct cggaggctat actgccgggc gtgacctccg tgatctcgac accgcggcgg
    18301 cgtatctgcg tgatggcggg tgtgacggcg aactcgtaca tcttgaaact ttagtcccgg
    18361 acagatggcg actacaaggt cggggccggc ggcccccgaa gaaactacaa gtatcggcta
    18421 agacccaccc gtctatcaag cgcgcgggcg gcggcagtag ccgcttcgtc cgtcccgcct
    18481 tcggcggctg tctgcccgcc cgactgcggc atccccgctc aactgagtac ttgttttgat
    18541 gcaagcatag acatttttat tgatttacaa tgagggattt aatagagtct gacgtgaatc
    18601 tgcccgtagc tcggatagat aactttcgtg aggaggtgac ctcatggcgg caaataaccc
    18661 agccgacatt caagaaataa tagacagcag gctcaagcgt cttgaagagc agataagcgc
    18721 ccttcacaaa agggtgaatg attttaggtt gattaatttt atccttagcg gcgtgacatt
    18781 agccggggta gtctcgttcg cattttggct aggcggcctc aacagtgaac tcaaacggaa
    18841 tacggccgaa cttgataagc tgaacaatac cgtcaaagcg ttaagcgata agctaatccc
    18901 ccctatggcc gttgtcgaaa cgaaaattga aaacttagat aggaagatag atcggattga
    18961 tgacaagcta acgggccaag tcgcacctca gagataatcc cgacctactt ttcgtacagc
    19021 cctcagactt ttcgtgcaat agtcatcttt cgggcaaagc tcatccggcc gaagtctgcc
    19081 cgccattggc aaaatggtcg ggcaaagccc catcggtgcg gcattctgac cggggtcggg
    19141 ctttcaaatt gaactgttga caggcggcgc ggtgaatcgt taaggtgaag ttaccgggcc
    19201 gcatggctcg cttctcccga aagtcttttg caatcttcta aaatcgagtc ttttcctcgg
    19261 aaacggacgc gccctcacgc ttttcgcgcg cggccacgtt tcgccccaaa gctgacccag
    19321 ccgcgaggca cggggattgc catttttagg tgcgcggagt ttcctcgcgc cccgccggat
    19381 tgtcttcaga agctcacgcc ggagaccaat gagcgccgcc gaattccatc ccccccacgc
    19441 tgtcaccccg ccacatgc
//
LOCUS       DASVES010000323        32326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1286643, whole genome shotgun sequence.
ACCESSION   DASVES010000323 DASVES010000000
VERSION     DASVES010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 32326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32326
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286643"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1052)
                     /locus_tag="VF240_13300"
     CDS             complement(186..1052)
                     /locus_tag="VF240_13300"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX7176232.1"
                     /translation="MTAHDDDEQRRQHTSAPDALDVDADADTTAAPALTEATPPAGDL
                     VRVRLLLFGAARDAAGGSAETTLEVAAPATVERALADILSRHPDLRRFGRSLLVAVNQ
                     EYALGQTELRDGDELALFPPVSGGGSDPSPASETNDAEDFFELTTEPINVGAVARRVV
                     PPHCGATVTLDGYAREWTRGRRTLYLTYEAYAPMALRELHRLGREAHQRFDIAHIGIV
                     HRTGRTDIGETSVVISVSAPHRRAAFEACEWAIRELKRTVPIWKKEFFEDGEVWVEGE
                     GAPEYVPGSEPN"
     gene            complement(1049..1747)
                     /locus_tag="VF240_13305"
     CDS             complement(1049..1747)
                     /locus_tag="VF240_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7176233.1"
                     /translation="MSESLERVREVRELPLFPLPLVLFPGVPLPLHIFEPRYRQMLAD
                     VLASNKLFGLSYHDPERGARPSVGHVGCAAEVVEVQPLDDGRSNLLAVGLVRYEVEEY
                     AEHDEPYLVGRVRFFEDEPEDEELLARRAREVSELFLRIANAVRALNDERAALPELPE
                     TEPERLSFLVAAAIEIDAEVKQQLLELRSSFERLSRLRDLLAQAVTGYEERARMHHVV
                     RGNGHSGKKVDFQE"
     gene            complement(1847..3256)
                     /locus_tag="VF240_13310"
     CDS             complement(1847..3256)
                     /locus_tag="VF240_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEX7176234.1"
                     /translation="MIKFLHISDVHLGCRRYNLDERTKDFFRAWFDVVERHAVANHVD
                     FVLIAGDFFDRRHIDPQTMNHAIAGLQKLKEAGIPAVVIEGNHDRRDSISPYSWMRSF
                     CQAGLLILLEPYNSGDSPIDLAPWDDEAREGSYIDIKGARIFGTHWLGMFNDVTMSIL
                     SDSIRRARDPNLFNILMLHTDVEGQLNRPGPALMPIGKLRELRPLVDYVALGHTHKRF
                     EVDGWAFNPGSLEACSVDEHREERGVYLVEVDDERRVTATHSRDYFQRPFQRIVFDVS
                     GAVDAEAVRVGVMEKVAAEARRHDADSTEPAPIIELILRGHLGFKNSLLELARMRDEA
                     RALTGALHVMVKNQSVPVEYAVAADLDADVTRAQRERRIVEDLIARDNRWRDRAPEMA
                     ELVLHAKKLILSDEAPADILKYIEDQLEKNASAAAIAPLEAEESEDLAPAPSAGAFQA
                     VARSAETSVSGLLSAVGDE"
     gene            complement(3500..4408)
                     /locus_tag="VF240_13315"
     CDS             complement(3500..4408)
                     /locus_tag="VF240_13315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7176235.1"
                     /translation="MSASFEIWEEKQPRLQLIESESKPFAEGNSPLEERLARLQLMAH
                     AMLDEVEQIRVSGVSTLEKEIEQGRDYYEVTNRFSAALIRRALRAAGGHQGRAARLLG
                     LKNTTLHSMIQRHNISPSAEYMGTSLRRATEADVPGLIDIARRSWLSAFAGTAPHKVI
                     RAWAEANHEALWYPEHWPRVTVAEVGGLSVGIVHHLGDEISGLWVRPSEQGCGVGTML
                     LRAVEQQIRAAKHTRAWLGNSSYNTRAHRFFAARGYAITRRCLDTSPAGAHGEIIVLE
                     RGLAPRGASGEQDGGEDFERRRQDSN"
     gene            5652..8912
                     /locus_tag="VF240_13320"
     CDS             5652..8912
                     /locus_tag="VF240_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7176236.1"
                     /translation="MTRAVRLFTVMLVVLFSMAGSAYAQETTGNIEVTVTDPTSARVP
                     GATVALTGGSLNRTVTADEQGVARIQQVPPGIYTVAISGGNFSGVTFDNVEVVLGKTT
                     PVNAALQAGGITESVVVSASDVAPIDPTDNKIQTNLTAQVLDQLPKGVGFATALRVAP
                     AVRPEPLGGGFQIDGASGAENTFIIDGQEVTNFRTGALNANNNIPFQFVQEVQVKSSG
                     FEAEFGGATGGVINVVTKSGSNDWRGEFGIQFRPGSLQSGFQPTGGDPVTLPARSFLR
                     RFNNAAGAQVVEYLTPRKDGGTDLFPTANLSGPIINNRLWFFGSFTPQFQHRERTLDY
                     ITSNPTTRQVVGTERYNRRQRNDYGFFRLDASVSDKLRLTGTYLWNPIAVRGALDNIT
                     TSVVADASGIPSADFGGQTGVLRGASFLDQQGGRQNSNNITGQATWTPTSNLVINFRG
                     GRSFLNEKLNSYGIPRSTRFICAAGTDTALALANGGCTPGQQNFASNFQIDYDVSVRK
                     TFDADASYLVSDFGGRHQFKFGYQYNGLSNATRQGYRNEGIVQLFYGFELGDVGASLD
                     CTDADHDGHCDAVGAGFIQRFATEGAASSSSQSFFIQDSWQPTNRLSLNLGLRFEKED
                     VPTFNADFPGINFGLGDKIAPRLGVAYDLTGDGKTKLFASYGWFYDRFKYELPRGSFG
                     GDFFRRDYFEIFPGSGSFQSFTSARILGTNSDVFGGQCPIANSTGLSVCQNDFRIPSN
                     RGLEFLFASGAVDPNLKAARQTEFTVGFERDLGSHFVLASRYTRKKVDRAIEDIGIPT
                     PEGSEAYIIGNPGMGLAAEVAAEAGFPSAKAQRQYDAFEVRLDKRFSNNYYFNVNYTY
                     SRLYGNYSGLASSDEGPNFGGQQGRNSPNVNRFFDLPFLGFTANGEPDNGRLATDRPH
                     VLKFNGSYNMPWTRFGSSRNSTDFQVFTTAQSGTPLTTVYNLYSTNVAILNGRGDLGR
                     TEMFTQTDIGISHTFRFGKSENKQFKLDLNILNLFNEANVLNRFTVISPTNFSGTTLS
                     TGDEIETINRIFNGGIRDLVTSAIAAAPNTRGEARYNQDNAFQLGRDIRFGFRFIF"
     gene            complement(9063..9347)
                     /locus_tag="VF240_13325"
     CDS             complement(9063..9347)
                     /locus_tag="VF240_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176237.1"
                     /translation="MVTRVSHMTGAPCTDCPRIFKATAIPATTLPSPPARDAIFQSFR
                     EVTAVVSGTDIQIAKVHLYKKYNYLDEFMMRDFYVTLWMDFVAVLLRAGV"
     gene            9464..13225
                     /locus_tag="VF240_13330"
     CDS             9464..13225
                     /locus_tag="VF240_13330"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7176238.1"
                     /translation="MTNLLPRRGRARTTILGALLLSLLACANVFAQSDTTTVRGTVRD
                     PQGGSVAGANVTLHSAARNFTRTQTTNAEGSYVFTAVPPDTYTVEVEVQGFKKVTVAD
                     VRALVDTPTDADVTLEVGAVSETITVTSANEAPLNTTDASLGNAFESQRIQELPLNAR
                     NVNNLLALQPGVTRQGEVNGGRRDQANFTLDGVDVNEQQTGLDVVNGSTATVPGDAFA
                     SVIRVNPDSVQEFRVTTSNPSASQGRSSGAQVSLVTKSGTNEWHGSAYEFHRNTATTA
                     NDYFNNALGKNPDGTDVAPRPKLLRNIFGGSIGGPIKKDRLFFFYSYEGRRDVEEQSV
                     LRNVPTATLRQGIVQYRNTDGELVQLSPTDIAALYPATGGVNPAALAILQTAPLPNDF
                     TVGDGLNRAGFRFNAPTSNNLNVHIARFDANLSDRHNLFFRAAYQDDLFEQPSMFPGT
                     PQPSLWVNPKAYAVGHTWTVTNSLVNNLRIGLTRAALTQQGDSSETAVIFRDVFQPRT
                     YQRTVSRTTPTWNFVDDVSWVKGNHAVQFGGNVRLIRNNRVDFQNSFDSALVNYQFYE
                     TGGSTVRNALADLDPDFEFDYGRAAVAVLGRFSQYSINSNFDASGQPLAGGSPALRSL
                     QTEEYELYGQDSWKVTPNLTLTYGLRWGVNTPVYERNGLQLVPTTILGDFLERRLASA
                     AAGTPLNELISFDLGGKANDRPGFYSTDKNNFAPMLAVAWSPDFGDNWFGRLIGRDGR
                     SVLRGGFRMLYDRVGSQLAVSAEAENSFGFNSETTNGSTSTNTSTRLGPLVTGLNPDV
                     RSFPRIDAPESLTFPLSFPADEVDRIIAGIDQALVSPVQYSWNVSYGRELPAGLSFEA
                     SYIGRSARNLLLVRDVMHLNNLVDSGSGADWYTAAGRLNDLRLADTPIESVGPIPYFE
                     NLFPGLGDSFFGDPSLSSTQAAYLLHAREEVGGLNFTDFTLLQLILDDAGSFPNAFFH
                     PQYAALQTLSSVGRSDYHGGTFSIRQRFRDKFLLDFNYTLSKSMDNASTLETLRVLSN
                     VVRNPIDTDLEYSVSDFDIRHNINANFLAQLPFGRGRAFGGDMGRVPNAILGGWQLSG
                     IMRWHSGLPAGSPGDFEWATNWQSQSNGVRLRDVRSGNCPDVDGHPNLFCNPTEAYRS
                     FRNARAGETGDRNISTLRLPSYFAMDMGLTKNFQMWYAEGHKLQFRWEVFNVTNTQRF
                     GVINTLGLNPEPFRANSAPEDFGRYIGSQTPVGETRPGRVMQFALRYEF"
     gene            complement(13613..14005)
                     /locus_tag="VF240_13335"
     CDS             complement(13613..14005)
                     /locus_tag="VF240_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7176239.1"
                     /translation="MKPKLLIVDDDVAITRQLFWMLCDEFEVLTAHDLQSALRRATVY
                     EPDVSIIDLHLPPTLNSTDTGMRLLEYVKGHLPASKVFVISSESGVETQKDCFRQGAD
                     DFLSKPLDVERLLSAVRRTAMEVQLSAA"
     gene            complement(14487..15899)
                     /locus_tag="VF240_13340"
     CDS             complement(14487..15899)
                     /locus_tag="VF240_13340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7176240.1"
                     /translation="MTEPADEKATVLVVDDDRVLRRQLNWALADTHRVLEAETRLEAV
                     EHLGRGGVDVVLCDLHLPPDLSGITEGLAVVEAARAMRPGVPVVVITGSDKKQAALEA
                     VRRGAYGFFEKPFDETELKHIVAQASRVRRLEEEVLRLRSELDMFRGFGRFVGRAPAL
                     EKILKQARAVADTNATVLITGENGTGKEIIARAIHEESARGVGPFIAVSCAALPEALI
                     ESELFGHERGAFTGAHAARKGRFELADTGTLFLDEIGELNPSMQVKLLRVLQERAFER
                     LGGTKTIEVDIRLVAASNLDLEEEVEAGRFRRDLFYRLNVVPLHLPPLRERREDVPLL
                     AAHFAAKAAEKHNRAGAELDPALLEALQEYDWPGNIRELENVVERLVVLTTGTRLCAE
                     HLPDKMLRQSAAHAAGETGAGAAGDETTLEGAMHALKRRLVRDAIESEGGNKAAAAKR
                     LQISRSYLHRLVSELGIEGG"
     gene            complement(15961..17907)
                     /locus_tag="VF240_13345"
     CDS             complement(15961..17907)
                     /locus_tag="VF240_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176241.1"
                     /translation="MGLRAKLLAAVALLAIVPATAIGVYNYTASARAVEALLREDVRA
                     RADAAARRATATLRAHEAHLTHVAGLSALRKYVLDSTTTAPEAAEPPEEVRAAVAALF
                     AGTSRLAAVSCLDGRGRPLFRASAPTSGPPATGTTFQTKDFITSTLRFDERVWSLTTA
                     HKEPLRAPSREPYGSGLRSTVPIFGPEDAPARPAGALVLETKLTALLEDATEESATAA
                     DDTSRATERVLFAVDTDSGLVVYHTNELLTGQTAVEAVPHFDSVRREAAAGATAFYET
                     PGGERWLAALARPEGLGVELVAAENYTRAVAGPRRAAWAGIVLPLLAGAVALALVFVL
                     TTRARRRIERVAEGAAAVAGGDLERRVEVDSSGATRVLAEGFNLMTERLREHLRREAE
                     TRQFESFMRLSAMLTHDLKNSITGLSMLVSNMERFYDHEGFRADAVSSLREATDKLRR
                     IVARLSEPVKSMSGEYRRDARPTDLVAVIRRVLAAHAEPSAPLYEIDKRLPAELIATV
                     DAERVENVVENLVVNALEAMGARGGRLTVEAGALAGDLVFFSVADTGAGMSEEFIRTR
                     LFRPFSTTKTKGIGLGLYTCREVVETHGGRLEVESNVGAGTRFRVVLPSALFTPHAQS
                     GQARKRTASSADATDATPDGQRDA"
     gene            complement(18058..18729)
                     /locus_tag="VF240_13350"
     CDS             complement(18058..18729)
                     /locus_tag="VF240_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7176242.1"
                     /translation="MQREQFAGAVKSSVSLVSPLERRMARAVVPRIPAWLETHHLTLL
                     TLLWCGGIVFFSRLAATTDLRWMWAVSLMILCQYLTDFFDGKVGKHRDTGLVKWGFYM
                     DHLLDYAFLCAVVVGYALVLPERALLSLLLVFAVYGAFMVNSFLTFAATERFEISHMK
                     LGPTEFRAALVVINALLVAAGPRPMKAALPYVAAGSLAALCVLVYRTQKQLWRRDMER
                     KAGSL"
     gene            complement(18907..19734)
                     /locus_tag="VF240_13355"
     CDS             complement(18907..19734)
                     /locus_tag="VF240_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176243.1"
                     /translation="MRATVQRFVNAAACAAVLFLLVAASVRVAAAQTPTAAPPPPPPR
                     ERLALMVVSVKPEMVAEFDAMMKNETNPALAKAGVKWRDVWNTATFGDRFTYYIVRPV
                     EKFADFDGPDPVAKGLGSPEAYAAWLAKAGRMVNSVRTTIIEGYPEMSHAPDMKEAPK
                     MAVVAFVSVTPGRNADYENHIKNEVLPVVKKSDVKGYWFHRTTLGGDANEYVIVTLHD
                     NYAELEKGPPQRRVLGPEGAMRLAQKLPAGTIAHMERIVLRLNPELTYRPNPPVSSR"
     gene            complement(20001..20333)
                     /gene="sugE"
                     /locus_tag="VF240_13360"
     CDS             complement(20001..20333)
                     /gene="sugE"
                     /locus_tag="VF240_13360"
                     /inference="COORDINATES: protein motif:HMM:NF008512.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX7176244.1"
                     /translation="MAWTLLIIAGVLEIVWAIGLKYTDGWTRLWPSVFTLAVMLIDFF
                     ILSAAMRSLPVGTAYAVWTGIGAVGTAAAGMILFGESREALRLACIGLIVAGIVGLKL
                     FTPAAAAP"
     gene            complement(20367..21233)
                     /locus_tag="VF240_13365"
     CDS             complement(20367..21233)
                     /locus_tag="VF240_13365"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX7176245.1"
                     /translation="MIAKRQPVEENAYERELGVALELARASGAAALEYYGGPLRVEHK
                     GDTDEPVTAADRAVNELIVTGLARAFPDDGILAEESVDTDRRLARARAWMVDPLDGTR
                     GFIAGSGDFAVQIGLAVEGRAVLGVVYAPATDVLYWAAPGVGAWVKRPGLDAERLRVS
                     DEKTVARMRIAESRSHRGPRMDAGVRALGVSAEVKRHSVGIKVGLLVERQCDLYIHLS
                     PRTKEWDTCAPEALLREAGGRMTDLWGEPLVYNRPDTLNRNGILATNGAAHESIVATL
                     APLLAGFGRARV"
     gene            complement(21339..22907)
                     /locus_tag="VF240_13370"
     CDS             complement(21339..22907)
                     /locus_tag="VF240_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7176246.1"
                     /translation="MSHFIDGRAKSASLDVSGEELRELSARACEMAAAYFARIAELPV
                     FPEAPAESLARLAGAELPVEGDTLARVLDECRAIFDASRHNGHPRMFGYVASPAAPVG
                     AFANLLASALNQNVTAWRSAPAATVVERTTVRWLAEMIGYADAEKSCGGLLTSGGSAA
                     NLTALFVAHRAHAARNSTGAGDAADLSQKGLWNAGAPATIYVSDQVHLSIPKAADILG
                     IGREQVRVVASDDNFRMDVRSLRESIERDARVGLRPFCVVASAGTVNTGAVDPLAEVA
                     AVAREHQLWFHVDGAYGALARLDPTKRALFDGLAEADSVSLDAHKWLYAPLDCGCLIL
                     RYPERARAAFAGTEEQYIKVYEQQDDEAFAFWDYGLELSRPFRALKVWAMLRYYGVAR
                     IGAAIAEDCALAEYMAERVGMAEDFELLAPVTLGICCFRYVSADARRALDAATESAAR
                     ASVNARLDDLNERVMQRVQRGGEAYLSNASLRGRFALRASITNFRTTRRDIDLTLDVV
                     RRVARELNQGEGNN"
     gene            23253..24374
                     /locus_tag="VF240_13375"
     CDS             23253..24374
                     /locus_tag="VF240_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7176247.1"
                     /translation="MSLEGRATQEGTARYRDRFPIASAEQHFRFEQGLWLSSVGVGTY
                     LGKANEETDRAYAEAAARAVELGANVIDTAANYRFQRSERSIGAALARLTSDGGFSRD
                     EIVICTKGGYFPFDGEPPRDVRSYVEETFVRTGIIDFSDIAAGSHCMTPAYLRNQVAQ
                     SLRNMGLGTIDVYYIHNPETQLSLVTRAEFETRLSAAFAALEGERAAGRVGYYGVATW
                     NGFRAKQGARDYHSLERMVELAREAGGADTHGFRFIQLPFNLSMPEALVADNQTVEGA
                     RMSTLEAARALGVTAIASASIMQGKVARDLPEHVREPLGSLSTDAQTAIQFVRSTPGI
                     TTALVGMSRREHVEENLQLARVAPASADDYRRLFEQGGE"
     gene            24642..25529
                     /locus_tag="VF240_13380"
     CDS             24642..25529
                     /locus_tag="VF240_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176248.1"
                     /translation="MLAVKELTKTYPSGVRALKGVTLEVPAGMFGLLGPNGAGKTTLM
                     KIAATLLEPDSGGVEIDGVDLIAHKKSARRMLGYLPQEFGLYPTLTAEQTLHYLAKLK
                     GVLDDKERRALIEALLERVNLSSARAQKVGGFSGGMRQRLGIAQALIGQPRMIIVDEP
                     TAGLDPEERVRFHNLLSEIAGENTVVILSTHIVSDVSNLCGRMAIIRQGEIIASCTPR
                     EAMGRITGAIWEATLPREKVLALKKSRLRIVSSQMFEGRVRVRAYSKDERPGEEFSPA
                     LPSLEDYYFSVAGQPESAN"
     gene            25526..27343
                     /locus_tag="VF240_13385"
     CDS             25526..27343
                     /locus_tag="VF240_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7176249.1"
                     /translation="MRSPTRTAFGAIFRNEVLLNSKRVAPYAMAALFGGNALLWWGWG
                     PAASRGLATNSDHFIAEMMPVFSFMTLPLFTAVIMGDPVIRDFRAGVTPLIFSKPVGR
                     ATYLLGKFFGNFFVLVCCQLVFPLTFFLFQWVPKSGLLVQPPRVLPYFKHFLILIVVT
                     HLVLAAVYFTVGTLTRNAKIVYGLAVSFYPLYISYQTLLLRSLPTRWRIALDPLLMNL
                     SLNARGRSAEFLNQLAVSYDSDVLANRAAMILAAGLFLTFLYVRFSMVERVRKEEDDG
                     RLTTIDLRPDRVVSGVESSSFDRPAQVEEVIRGERVAIPKVGTASEGVGASLEQLTAA
                     LGIELRLLSAERGLVVLVPTATLLCVAGLAYFEAVPGETHTAAYAARTAESVLLFLAA
                     IAVFFTGEATARDRELRVEPVLWSAPAPNFVLLLSKFSATLLLCVAFSLVVGLAAIAL
                     QIFRGHTPFEISAYLKAYSLILIPSIVFLTAAAVALNVLLRDKYVAYAMSFAVGGALL
                     YLFNNGYNHWLYNPVLYGLWTPADLTDAASNQTLIITHRIYCLAMASLLLSLAHLCFE
                     RKSTKGLRSQGRLSGAGWSLLIMSLSTVTAVITGLMINV"
     gene            27414..27752
                     /locus_tag="VF240_13390"
     CDS             27414..27752
                     /locus_tag="VF240_13390"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7176250.1"
                     /translation="MLNPTDDFYCDEALSGRTPVRKVAETDNVLAFHHTRPFYPAHVV
                     VIPKRHIASLLALDAEDDELLLELLAVVRRVAAEVLAEHGACRVITNLGEYQDSKHLH
                     WHVVSGQPLR"
     gene            complement(27843..28259)
                     /locus_tag="VF240_13395"
     CDS             complement(27843..28259)
                     /locus_tag="VF240_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176251.1"
                     /translation="MKKALLLGAIVMFFGLGAADTFGQRNDRQRIRRGVRSGEITRDE
                     ARVLRERQRQNRTERRGYRSDGTITREERRDIRRDERQHDRQIRRARHNDDRRDGYDN
                     RRNNHRRGNGYYRRGAGSRNHPVFGSDNRRRGRGRN"
     gene            28672..29025
                     /locus_tag="VF240_13400"
     CDS             28672..29025
                     /locus_tag="VF240_13400"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEX7176252.1"
                     /translation="MSQRETRTFAEFWPFYVMEHSRPGTRLLHAAGTLGSTTLLVALI
                     ATGRWRWLPLVLVVGYGAAWVGHFFVEHNRPATFAHPLWSFAADYKMVWMMLTGRMSA
                     EVERSRAVRAAARQS"
     gene            complement(29000..30355)
                     /locus_tag="VF240_13405"
     CDS             complement(29000..30355)
                     /locus_tag="VF240_13405"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7176253.1"
                     /translation="MNTALKLFSFLLLTLVVFSVEARACSCAGSAPPCEAYWRASAVF
                     VGQVVGVSNVRVEEGEGENKHEQGWRAFRFAVERPLRGVADAYVEVLTGGGGGDCGYG
                     FERGGRYIIYAYRDEKTQRLYTGICSRTQPVAEAGEDLRYIDGLSQGPPAGGAIYGQV
                     LRARREKDDEGSERDASVPVSGAKIEAAGKGKTFRAETDAEGKYRFGGLPPGEYVLKL
                     DLPKTLMVNEPERKVRIDERGCAVASFYADENGRLSGRVFDAAGRPASRVSLQLSAAE
                     SGEQSLRGPVGYATTDAEGRYEVKGIPPGRYIIRIRFDGEPTASRPFPVLYHENVTEA
                     ARARVVQISEGEHVEDYDIRLPPLPGERVVSGVVVWPDGQPAAGVTISYTSDIPNMLI
                     GDAATADEAGRFSFKVYEGIGMKLSVSVNRADGSWVNSNIANVPATGAVGEIRIVVPR
                     P"
     gene            complement(30384..32189)
                     /locus_tag="VF240_13410"
     CDS             complement(30384..32189)
                     /locus_tag="VF240_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7176254.1"
                     /translation="MRPKAIVSLLLVCVVHVVSLGQSVGGVESRIGRVEKGLLTAVVI
                     KGDPTWSIEERMRHYKAPAVSVAVFKDFKVEWARAYGLKDVGTKEAATAETLFQAGSI
                     SKSVAAMVALRKVQEGKLALDENINNRLTSWKLPDNEFTAKKKVTLANLLSHSAGTTV
                     HGFPGYAVSEKLPTLPQVLDGAAPANTAAVRVDLEPGTRFRYSGGGTTIAQLAVMDVE
                     RKSYPQIAKETVLGPVQMTNSTYSQPLPDDWRPRAATGYKPDGSEVKGKIHVYPEMAA
                     AGLWTTPTDLAKFAIEVQLSLAGKSNKVLSKESVERMVTPFASENVGMGFFIQKHGAA
                     TYFGHGGADEGFRAMLLAHRERGYGAVVMVNSDNGQIMEEIVRSIAREYAWEDYLSAP
                     FEVTTLAPERLDAYVGRFRINPDRLLNVTKEGGRLYAQPTQGPKVELLPISEDTFIRR
                     DQNVQYTFVRNAEGRAGAIRVKSDGGANEAARVGDDVLIPYDLLMSGKTAEALAGYRQ
                     IRKEQPANVAVREERLNLLGYELMRQKKLAEAIAVFKLNVEFYPQSFNTYDSLGEAYM
                     ENGERELAIASYKKSLELNPKNDNGVQMLKKLEGK"
BASE COUNT         5451 a        10791 c        10316 g         5768 t
ORIGIN      
        1 atacgtcggg agataaaaat gaagaggcaa tgtcgtgcaa agtagagctg gggattctaa
       61 taggtaaatc gctcaaagta atctcctctc aatgtgagcg gaataatagc attgctatga
      121 attgcaacaa ccgaacgcga attagacgcc ctaacagccg ccggtaagga cgccggattc
      181 ggcgtctaat tcggctctga ccctggtacg tactcgggtg cgccctcgcc ttcgacccag
      241 acttcgccgt cttcgaagaa ctcttttttc cagatgggca cggtgcgttt gagttcgcgg
      301 atggcccact cgcaggcttc gaaggcggcg cggcggtggg gggcgctgac ggagatgacg
      361 acggaggtct cgccgatgtc ggtgcggccc gtgcggtgga cgatgccgat gtgggcgatg
      421 tcgaaacgct gatgggcctc gcggccgagg cggtgcagct cgcgcagcgc catcggggcg
      481 taggcttcgt aggtgagata gagcgtgcgg cggccacgcg tccactcgcg cgcgtagccg
      541 tcgagtgtga cggtggcgcc gcagtggggg gggacgacgc ggcgggcgac cgctccgacg
      601 ttaatcggtt cggtcgtcag ctcgaagaag tcttcggcgt cgtttgtctc ggaggcgggt
      661 gaggggtcag agccgccgcc gctgacgggg gggaagaggg ccagctcgtc gccgtccctg
      721 agttcggtct ggcccagcgc gtactcctga ttgacggcga cgaggagcga gcggccgaag
      781 cgtcggaggt cggggtgacg cgagagtatg tcggcgagcg cgcgctcgac cgtcgcgggc
      841 gcggcgactt cgagggtggt ctcggcggag ccgccggccg catcgcgcgc cgcgccgaag
      901 aggagaagcc ggacgcgaac caggtcgccg gccggcggcg tcgcttcggt tagtgcgggc
      961 gcggcggttg tatcggcgtc ggcgtctaca tcgagtgcgt cgggggcgga cgtgtgttgc
     1021 cttcgctgct cgtcgtcgtc gtgcgcggtc attcctgaaa atccaccttc ttgccgctgt
     1081 ggccgttgcc tctgacgacg tggtgcatgc gcgcgcgctc ctcgtagccg gtgacggcct
     1141 gcgccagaag gtcgcgcagg cggctcaggc gctcgaagct ggagcgcagc tcgaggagct
     1201 gttgcttgac ctcggcgtcg atctcgatgg ccgccgccac gagaaacgag aggcgctccg
     1261 gttccgtctc gggcagctcc ggcagcgcgg cccgctcgtc gttgagcgcc cgcacggcgt
     1321 tggcgatgcg gaggaacagt tccgacacct cgcgcgcacg ccgcgcgagc agctcctcgt
     1381 cttccggctc gtcctcgaag aagcggacgc ggccgacgag ataaggctcg tcgtgctcgg
     1441 catactcttc gacctcgtag cgcaccagtc cgacggcgag caggttcgag cgcccgtcgt
     1501 cgagcggctg cacttcgacg acttcggccg cgcagccgac gtggcccacg ctcgggcgtg
     1561 cgccccgctc ggggtcgtgg taggagaggc cgaacagttt gttcgaggcg agcacgtcgg
     1621 cgagcatctg ccggtagcgc ggctcgaaga tgtgtagcgg gagcggcacg ccggggaaca
     1681 gcacgagcgg cagcgggaac aatggcagct cccgcacctc gcgcactctc tcaagcgatt
     1741 cggacatgat gagcggtcag cggtaaagaa attagctgtt agctgttagc gggcagctaa
     1801 cggcggaagt cgtatcgggt agagcgtgct ggagtctcac gcgcggtcat tcgtcgccga
     1861 cggctgaaag caggccgctg acggatgttt cggcgctgcg tgcgacggcc tgaaaagcgc
     1921 cggccgaggg cgcgggcgcg aggtcttccg attcttcggc ctcaaggggg gcgatggcgg
     1981 cggcgctcgc gttcttttcg agttgatcct cgatgtactt gaggatgtcg gccggggctt
     2041 cgtcgctgag gatgagcttc ttggcgtgga gcaccagctc ggccatctcg ggggcgcggt
     2101 cgcgccagcg gttgtcgcgc gcgatgaggt cttcgacgat gcggcgctcg cgttgcgcgc
     2161 gcgtcacgtc cgcgtcgagg tcggcggcga cggcgtactc cacgggcacg ctctgattct
     2221 tgaccatgac gtgaagcgcg ccggtgagcg cgcgcgcctc gtcgcgcatg cgcgcgagtt
     2281 cgagcaggga gttcttgaag ccgaggtggc cgcgcaggat gagttcgatg atcggcgccg
     2341 gctcggtgga gtctgcgtcg tgccggcgcg cttccgcggc gaccttttcc atgacgccca
     2401 cgcggacggc ctcagcgtcc acggctcccg atacgtcgaa gacgatgcgc tggaacgggc
     2461 gctggaagta gtcgcgcgaa tgtgtggccg tgacccggcg ctcgtcgtca acttcgacga
     2521 ggtagacgcc gcgctcctcc cggtgctcat ccacggagca ggcttcgagc gagccgggat
     2581 tgaaggccca gccgtccacc tcgaaacgct tgtgcgtgtg accgagcgcg acgtaatcga
     2641 cgagcgggcg cagttccctc agcttcccta tcggcatcaa ggccgggccg ggccggttga
     2701 gctgtccttc cacgtccgtg tggagcatca ggatgttgaa gaggttgggg tcgcgcgcgc
     2761 gccggatcga gtcggagaga atcgacatcg tcacgtcgtt gaacatcccc agccagtgcg
     2821 tgccgaagat gcgcgcgcct ttgatgtcga tataggaacc ttcgcgcgcc tcatcgtccc
     2881 acggtgcgag gtcgatgggg ctgtcgccgc tgttataggg ttcgagcagg atgagcagcc
     2941 cggcctgaca gaacgaacgc atccaactgt agggcgagat ggagtcgcgg cggtcgtgat
     3001 tgccctcgat gacgacggcc gggatgccgg cctctttgag cttctggagg cccgcgatgg
     3061 cgtggttcat cgtctgcggg tcgatgtgcc ggcggtcgaa aaagtcgccc gcgatgagaa
     3121 cgaaatccac atggttcgcg acggcgtgac gctcgaccac gtcgaaccac gcgcggaaga
     3181 aatctttcgt ccgctcgtcc agattgtagc ggcggcagcc caggtgaacg tccgagatat
     3241 gcaggaattt aatcatcgat tccgatggcg gcttgtcgaa taaatcgcgt cgaagaggtc
     3301 gcttcaagta ctcacggcac gcatctatta tacgacatcg gggcgggaaa agccttaaag
     3361 gccgtaaatc gtcaatcgtg aatcgggaat cgcggttaag aggtcaaacg ccttgagcga
     3421 tcagcactca ggcggcgaca accaccacga ttgacgattc acgagtgacg atttacggcc
     3481 tttgacgatt gcggctcgtc taattcgaat cttgtcggcg acgctcgaag tcttctccgc
     3541 cgtcttgttc gcccgacgcg ccgcggggcg ccagcccacg ctctaagacg atgatttcgc
     3601 cgtgcgcgcc ggccggcgat gtgtcgagac aacgccgcgt gatcgcatag ccccgcgccg
     3661 caaagaagcg gtgggcgcgc gtgttgtaag aggagttgcc gagccacgcc cgcgtgtgtt
     3721 tggcggcgcg tatctgttgc tcgacggccc tgagcagcat cgtgcccacg ccgcacccct
     3781 gttccgacgg gcgcacccac aaaccgctaa tctcatcccc gagatgatgg acaatgccta
     3841 cggacaggcc cccgacctcg gccacggtca cgcgcggcca atgctccgga taccagagcg
     3901 cctcatggtt cgcctccgcc cacgcgcgaa tcaccttgtg cggggccgtc ccggcgaagg
     3961 cgcttagcca ggagcggcgg gcgatgtcga tgagtccggg cacgtccgcc tcggtcgccc
     4021 gccgcaggct cgtccccatg tactccgccg acggcgagat gttgtgacgc tggatcatgg
     4081 agtggagggt cgtgttcttt agcccgagca gtcttgcggc ccgcccctga tgaccgcccg
     4141 cggccctgag cgcccgcctg atgagcgcgg cgctgaagcg gttcgtcact tcataataat
     4201 ctctcccctg ctcgatctcc ttttccaggg tgctgacccc ggagaccctg atctgctcca
     4261 cctcatcgag catggcgtgc gccatcagtt ggagacgcgc gagcctctcc tcaagcggcg
     4321 agttgccttc ggcgaagggc ttcgactcgc tttctattaa ctgcaacctc ggttgcttct
     4381 cctcccaaat ctcgaaactt gcagacatca accaccccct tatcaaagtt ttctgtcacg
     4441 cggtgtcgcc gctgcccacg gcgacaccgc ggtcgcggcg gcaacacgtc agaccggacg
     4501 gcggcggacg ctcatgaacg gccgcggcac gttcgggact tcgagcgacg gttcaaaagc
     4561 gcccgcgctc gtcggcggtg aagacgggga tgtgcgcctc ctccgtgaag aggcgctcga
     4621 cgccgtcggc cggcgtcggc cggactccgc gcggcgcgtg tccggtggtg ggcagaacag
     4681 gaacggaagc gaaaaaccgt agtcagaatc tgcgcgcggg atcatagtta aaactccgcg
     4741 gtcggggcgc tgcttttttt tgaagactct gatgccggcc gtggcggtgc ggtcgaaggg
     4801 cgaaagcggg aggccatccg gtgctccagt tcgtcaagtg tctggcctga acgacgcccc
     4861 cacgagcctc cgacatctat tgtgtgaaca ccgcaccgtc tattgtgtga acaccacacc
     4921 gtctgacttc gtggcagacg tttgatcggc cacgtcccgc cgtacaaccc acgtcgtcgt
     4981 tcaaagcttg aacgtcttcg ggcaaccggc ggttgaattt tttttagtgg ccgccggccg
     5041 acgcgccacc cgagtcacgt cttaaacatg ttccccagac tatgttctcc agcgccccag
     5101 cgaggttcca aattcttcta acgtgataat tctccgccgc atgtggccgg gggaaaacaa
     5161 aaatgtgata aggcggtaga tgccgggtgc gagccgcacc gcaggcgata agaaaatcaa
     5221 ttcaatgtac ttatttctca tttcgtaatg atgtgtggtc acgccgcggg cgatgagaag
     5281 ggtcgtgatc gactctgcgt gaagcatcag gctcaccgcg cggtcgtatg tgtatacgaa
     5341 atctcgcaac ctcgtttttg atcaggctcc gaaggtttgt agattaaatc caggaatcca
     5401 tatgacgggg cgctcacttg attgcgaaac gacggcggag ccttcacccg cacaaccctt
     5461 tcacgtcttc gcgctctgag ggcatgggca ttgcctagtt cattcgtgtg aggacacgac
     5521 atggtctagt cctcgaattc gcccacatcg ttttttcaga ttttcgaaga ggctttgtcc
     5581 tcttacaggg agtagtgcgc cttcagcggg cggccctgct ccgccaacaa atcagtaagg
     5641 agtatcaggt gatgacaaga gcagtcagat tatttacagt catgctggta gttctcttta
     5701 gcatggcggg aagcgcctac gctcaggaga cgaccggcaa catcgaagtc acggtgacgg
     5761 acccgacatc ggcgcgcgtg cccggcgcga ccgtcgccct cacgggcggc tctctcaacc
     5821 gcaccgtcac ggctgacgag cagggcgttg cccgcatcca acaggtgccg cccggcatct
     5881 acaccgtggc catcagcggc ggcaacttca gcggggtgac cttcgacaac gtcgaggtcg
     5941 tgctcggcaa gacgacgccc gtcaacgcgg cccttcaggc cggcggcatc accgagtcgg
     6001 tggtcgtctc ggcctcggac gtggccccca tcgacccgac cgacaacaag attcagacca
     6061 acctgacggc tcaggttctc gatcagctcc cgaagggcgt cggcttcgcc accgcgctca
     6121 gggtcgcccc ggccgtgcgc cccgagccac tcggcggcgg cttccagatt gacggcgcca
     6181 gcggcgccga gaacacgttc atcatcgacg gacaggaagt caccaacttc cgcaccggag
     6241 cgctgaacgc caacaacaac attccgttcc agttcgtcca ggaagtccag gtcaagagtt
     6301 cgggcttcga ggcggagttc ggtggggcca cgggcggcgt catcaacgtc gtcaccaaga
     6361 gcggctccaa cgactggcgc ggtgagttcg gcatccagtt ccgccccggc tcgctgcaat
     6421 cgggattcca gcccacgggc ggcgaccccg tgacgctgcc ggcgcgtagt ttcctgcgcc
     6481 gcttcaacaa cgctgccggc gcgcaggtgg tcgaatatct caccccgcgc aaagacggcg
     6541 gcacggacct cttcccgacg gccaacctga gcggccccat catcaataac cggctgtggt
     6601 tcttcggcag cttcacgccg cagttccagc accgggagcg cacgctcgac tacatcacct
     6661 cgaatcccac cacccggcag gtcgtgggga cggagcgcta caaccgcaga cagcgcaacg
     6721 actacggctt cttccgtctc gacgcttcgg tctccgacaa gctgcgtctg accggcacgt
     6781 acctgtggaa tccgattgcc gtcaggggcg cgctcgacaa catcacgacc tccgtggtgg
     6841 cggacgcctc cggcatcccg agcgccgact tcggcggcca gacaggcgtg ctccgcggcg
     6901 cctcgttcct ggatcagcag ggcggccgtc agaactccaa caacatcacg ggtcaggcca
     6961 cctggacgcc gacctccaat ctggtcatca acttccgcgg cggccgcagc ttcctcaacg
     7021 agaagctcaa ctcttacggc atcccgcgct cgacgcgctt catctgcgcc gccggcaccg
     7081 acaccgcact cgccctggca aatggcggct gcactccggg gcagcagaac ttcgcctcga
     7141 acttccagat cgactacgac gtgtcggtcc gcaagacctt cgacgccgac gccagctatc
     7201 tggtctccga cttcggcggc cgccaccaat tcaagttcgg ctaccagtac aacggcctga
     7261 gcaatgcgac ccgccagggc taccgcaacg agggcatcgt ccagttgttc tacggctttg
     7321 aactcggcga tgtcggcgcc agcctcgact gcacggacgc cgaccacgac ggtcattgcg
     7381 acgccgtcgg ggcgggcttc atccagcggt tcgccaccga aggcgcggcc agcagctcca
     7441 gccagtcctt cttcattcag gatagctggc agcccacgaa ccgcctctcc ttaaacctcg
     7501 gcctccgctt cgagaaggaa gacgttccga ccttcaacgc cgacttcccc ggcatcaact
     7561 tcggcctcgg cgacaagatc gccccgcgcc tcggcgtggc ctacgacctg accggcgacg
     7621 gcaagaccaa gctcttcgcc tcctacggct ggttctacga ccgcttcaag tatgagttgc
     7681 cgcgcggcag cttcggcggc gatttcttcc gccgcgacta cttcgagatt ttccccggca
     7741 gcggctcctt ccagagcttc acctccgcgc gcatcctggg caccaactcg gacgtgttcg
     7801 gcgggcagtg cccaatcgcg aactcgacgg ggctgtcagt ctgccagaac gacttccgca
     7861 tcccgtccaa ccgcggtctt gagttcctgt tcgcgagcgg ggccgttgac ccgaacctga
     7921 aggcagcgcg ccagacggag ttcacggtcg gcttcgagcg cgacctcggc agccacttcg
     7981 ttctggccag ccgctacacc cgcaagaagg ttgaccgcgc catcgaggac atcggcatcc
     8041 cgacgccgga aggctccgag gcctacatca tcggcaaccc cggcatgggg ctggccgccg
     8101 aagtcgccgc cgaagccggc ttcccgtcgg cgaaggcgca acggcagtac gacgccttcg
     8161 aggtgcgcct cgacaagcgg ttctcgaaca actactactt caacgtcaac tacacctaca
     8221 gccgtctcta cggcaactac tccggcctcg ccagctccga cgaaggcccg aacttcggcg
     8281 gtcagcaggg ccgcaacagc ccgaacgtga accggttctt cgacctgccg ttcctcggct
     8341 tcacggccaa cggcgagccg gacaacgggc gtctcgccac cgaccgcccg cacgtcctga
     8401 aattcaacgg ctcgtacaac atgccgtgga cgcgcttcgg cagctcgcgc aactcgacgg
     8461 acttccaggt cttcacgacg gcccagagcg gcacgccgct gaccaccgtc tacaacctgt
     8521 acagcacgaa cgtggcgatc ctcaacggcc gtggcgacct cggccgcacg gagatgttca
     8581 cgcagacgga cattggcatc agccacacct tccggttcgg gaagagcgag aacaagcagt
     8641 tcaagctcga cctcaacatc ctgaatctct tcaacgaggc caacgtgctc aaccgcttca
     8701 cggtcatcag cccgaccaac ttcagcggca ccacgctcag caccggcgac gagatcgaga
     8761 ccatcaaccg catcttcaac ggcggcatcc gcgacctcgt cacctcggcc atcgccgcgg
     8821 ctccgaacac ccgcggcgag gcgcgctaca accaggataa cgcgttccag cttggccgcg
     8881 acatccgctt cggcttccgc tttatcttct aagccgaacc ttcacgcgta aactcagtag
     8941 cccatcccgc cgccgggatg ggctacattt ttttctggcg caatcccggt caaaggacca
     9001 gacggtgaag ttgttcgagt gcaggtccac accgggcttt gattacacag ttcatgccgc
     9061 attcaaaccc ccgcccgtaa aagaaccgcg acaaaatcca tccataaagt tacataaaaa
     9121 tccctcatca taaactcatc cagatagtta tattttttat aaagatgcac tttagcaatt
     9181 tggatatcag tgccgctgac gaccgccgtc acctctctaa acgactgaaa aatcgcgtct
     9241 cttgcgggag gtgaaggaag tgtcgtggct ggaatggcgg ttgccttaaa aattctcgga
     9301 caatccgtgc atggtgcgcc tgtcatgtga gagacacgcg tgaccatcga cctcgtcgga
     9361 cacgtcggct ccgcccaccg gccacttccc gacacatatt tccctgcctc cggcgctcaa
     9421 cgaaaacgcc gcgggcggcc atgcgagcga ggaggttaga atcatgacga acttgttgcc
     9481 ccgcagggga cgcgcccgaa cgacgattct cggggcgctg ctgctctccc ttctggcctg
     9541 cgccaatgtc ttcgcgcaga gcgacaccac gactgtccgc ggcacggtgc gcgacccgca
     9601 gggcggttcc gtcgccggcg cgaacgtcac cctgcacagc gcagccagaa atttcacacg
     9661 cacccagacc accaacgcgg aaggaagcta cgtcttcacc gccgtccccc ccgacaccta
     9721 caccgtcgag gtcgaggttc agggcttcaa gaaggtgacg gtcgccgacg tgcgcgcgct
     9781 cgtcgatacg ccgaccgacg ccgacgtgac gctcgaagtc ggcgccgtca gtgagacgat
     9841 caccgtcacg tccgccaacg aggcgccgct caacacgacc gacgccagcc tcggcaacgc
     9901 cttcgagtcg cagcgcattc aggagttgcc cttgaacgcg cgcaacgtca acaatctgct
     9961 cgcgctccag cccggcgtca cgcgtcaggg cgaggtcaac ggcggccggc gcgatcaggc
    10021 caacttcacg ctcgacggcg tcgatgtcaa cgagcagcag accggcctcg acgtggtcaa
    10081 cgggagcacg gccaccgtgc ccggcgacgc cttcgccagc gtcatccgcg tcaaccccga
    10141 ctccgtacag gagttccgcg tgacgacctc gaacccgtcg gccagccagg gccgctcctc
    10201 cggcgcgcag gtctctttgg tcaccaagtc gggcaccaac gagtggcacg gctcggccta
    10261 cgagttccac cgcaacacgg cgacgaccgc caacgactac ttcaacaacg ccctcggcaa
    10321 gaaccccgac ggcacggacg tggccccgcg cccgaagctt ctgcgcaaca tcttcggcgg
    10381 ctccatcggc ggccccatca aaaaagatcg cctctttttc ttctacagct acgaggggcg
    10441 gcgcgacgta gaggagcagt cggttttgcg taacgtcccg acggcgacgc tgcgccaggg
    10501 catcgtccag tatcgcaata cggatggcga gctcgtccaa ctctccccga ccgacatcgc
    10561 cgcgctctac ccggcgacgg gcggcgtcaa cccggccgcg ctcgccatcc tccagaccgc
    10621 tccgctgccg aacgacttca ccgtcggcga cggcctcaac cgcgccggct tccgcttcaa
    10681 cgcgccgacc tccaacaacc tcaacgtcca catcgcgcgc ttcgacgcga acctgagcga
    10741 ccggcacaac ctgttcttcc gcgccgccta tcaggatgac ctcttcgagc agccctcgat
    10801 gtttcccggc acaccgcagc cgagcctctg ggtcaacccg aaggcttacg ccgtcggcca
    10861 tacgtggacg gtcaccaact cgctcgtcaa caatctgcga atcggactga cgcgcgccgc
    10921 gctgacgcag cagggcgaca gcagcgagac ggccgtcatc ttccgcgacg tgttccagcc
    10981 gcgcacctac cagcgcaccg tcagccgcac cacgccgacg tggaacttcg ttgacgacgt
    11041 gtcatgggtc aagggcaacc acgccgtcca gttcggcggc aacgtccgtc tcatccgcaa
    11101 caaccgcgtc gatttccaga actcgttcga cagcgcctta gtcaactatc agttctacga
    11161 gacgggcggc tccaccgtcc gcaacgcgct cgccgacctc gaccccgatt tcgagttcga
    11221 ctacgggcgg gcggccgtcg ccgtcctcgg tcgcttctcg cagtacagca tcaactcgaa
    11281 cttcgacgcc tcgggccagc cgctcgccgg cggcagtccc gccctgcgct cgctccaaac
    11341 cgaggagtac gaactctacg ggcaggacag ctggaaggtg acgcccaacc tgacgcttac
    11401 ctacggcctg cgctggggcg tcaacacgcc cgtctacgaa cgcaacggcc tccagctcgt
    11461 cccgaccacc atcctcggcg atttcctcga acgccgtctg gcgagcgccg ccgccggcac
    11521 acccttaaac gaattgatct cgttcgacct cggcggcaag gccaacgacc ggcccggctt
    11581 ctactcgacc gacaagaata acttcgcccc gatgttggcc gtcgcctggt cgcccgactt
    11641 cggcgacaac tggttcggcc gcctcatcgg ccgcgacgga cgctcggtgc ttcgcggcgg
    11701 cttccgcatg ctctacgacc gcgtgggcag ccaactcgcc gtcagcgccg aggccgagaa
    11761 ctccttcggc ttcaactccg agaccaccaa cggctcgacc tcgacgaaca cctcgacgcg
    11821 gctcggcccg ctcgtcacgg gccttaaccc tgacgtgcgc tccttcccgc gcatcgacgc
    11881 gccggagtcg ctcaccttcc ccctctcctt cccggccgac gaggttgacc gcatcatcgc
    11941 cggcatcgac caagcgctcg tctcgcccgt ccaatactcg tggaacgtct cctacggccg
    12001 cgagctgccc gcgggactct ccttcgaggc gagctacatc gggcgctcgg cccgcaacct
    12061 cctgctcgtg cgcgacgtga tgcacctcaa caacctcgtc gatagcggct cgggcgctga
    12121 ctggtacacg gccgccgggc gcctcaacga cctccggctc gccgacacgc ccatcgagag
    12181 cgtcgggcct atcccgtact tcgagaacct cttccccggc ctcggcgact ccttcttcgg
    12241 agacccgtcg ctgtcttcga cgcaggccgc ctacctcctg cacgcgcgcg aagaagtcgg
    12301 cgggctgaac ttcaccgact tcacgctcct gcaactcatc ctcgacgacg cgggtagttt
    12361 cccgaacgcg ttcttccacc cgcagtacgc ggccctccag acgctctcga gcgtcggccg
    12421 ctccgactac cacggcggca cgttctccat ccgccagcgc ttccgcgaca agttcctcct
    12481 cgacttcaac tacacgctct cgaagtcgat ggacaacgct tcgacgctgg agacgctgcg
    12541 cgtgctctcg aacgtggtcc gcaaccccat cgacaccgat ctcgaatact cggtctccga
    12601 cttcgacatc cggcacaaca tcaacgccaa ttttctcgcc cagctcccgt tcggccgcgg
    12661 ccgggcgttc ggcggcgaca tgggccgcgt gccgaacgcc atcctcggcg gctggcagtt
    12721 gtccggcatc atgcgctggc actcggggct tccggccggc tcgcccggcg acttcgagtg
    12781 ggcgaccaac tggcaatcgc agagcaacgg cgtgcgcctc agagacgtgc gttcgggcaa
    12841 ctgccccgac gtggacggcc acccgaacct cttctgcaac ccgacggaag cctaccgcag
    12901 cttccgcaac gcgcgcgccg gcgaaaccgg cgaccgcaac atctcgacgc tgcgccttcc
    12961 ctcctacttc gcgatggaca tgggtctgac gaagaatttc cagatgtggt acgccgaagg
    13021 ccacaagctg caattccgct gggaggtctt caacgtcacc aacacgcagc gtttcggcgt
    13081 catcaacacc ctcggactca accccgagcc gttccgcgcc aactccgcgc ccgaagactt
    13141 cggacgctac atcggctcgc agaccccggt cggcgagacg cggcccggac gcgtgatgca
    13201 gttcgccctg cgttacgagt tctgattcct tgcgcggccg caaggactgc aagcgggccg
    13261 ggattgctcc cggcccgctt tttttggcgg caaattttgg cagtaatcgt gaaggacaga
    13321 aacgaagcgc gttcgcgttt tccaaaatcg gggtgaggga ggtagtggga acccgctgtc
    13381 gtcggaaaaa aaataaagcc cccgcgactc tgggcgagag tcttatttgg aagcgggggc
    13441 tttaacgcac cgcgcgccgg cgaagggata ccgtatgtgc gcgggcgtct gcaacggccc
    13501 gctagaaggg gtgtgcgggc cgtaaccgtg aagttgtgaa agatcaaaga gcgggtgaag
    13561 cgctgctggg gtgaaacttg tgttagaggc cgcggcggct cgccgggcgg actcaggcgg
    13621 cgctcaactg aacttccatc gccgtgcgcc gcacggccga gagcagtcgc tcgacatcga
    13681 gtggcttgct caggaagtcg tccgcgccct gccggaagca gtccttttgc gtctcgacgc
    13741 cggactccga gctgatgacg aagaccttcg acgcgggcag gtggcccttg acgtattcga
    13801 gaaggcgcat ccctgtgtcg gtcgagttca gcgtcggcgg cagatgcagg tcgatgatcg
    13861 acacgtccgg ctcgtagacg gtcgcacgac ggagggccga ctggagatcg tgcgccgtca
    13921 gcacctcgaa ctcgtcacac agcatccaga agagctgccg cgtgatggcc acgtcgtcgt
    13981 caacaattaa aagcttcggc ttcatcgact ctccgcctac atacttatgc gacttccggc
    14041 aagacatttc atgaggctgc atgtgcgtgg ctgaaaatgt agcttttagt ttcaatccga
    14101 ttcactaccc tcgcgcacat tgcgcgccct ttgggatcgc gggcgccggc cgcgcacgct
    14161 ttattcgcaa cacgcgtgcc aacgggcaag cgggcgcaaa ggcgtgattt tgccactaaa
    14221 tttgcgtcgc ctgggcggac ggaaggccga agtgtctacg ggagtgaacg gcccggccat
    14281 ccgcatgtgg acatttgttg actgtcgagg ggcgggggaa agcagggatg agggcggagg
    14341 gcggagggat gagggatgaa ggcgcgtcgg caggaggcag cgggcaggag gcagtcaagg
    14401 cgctcgaccg gcatggactg ctcactgctc actgctcact gcttactatc ttctttcatc
    14461 cctccgccct ccgccctcat ccctcgtcag ccgccttcga tgcccagctc gctgacgagg
    14521 cggtggaggt aagagcggct gatctggagg cgcttggcgg cggcggcctt gttgccgccc
    14581 tcactctcga tggcgtcgcg gacgaggcgg cgcttgagcg cgtgcatggc cccttcgagc
    14641 gtggtctcgt cgccggcggc gcccgcgcct gtctccccgg cggcgtgcgc ggccgactgt
    14701 cggagcatct tgtcgggcag gtgctccgcg cacaggcgcg tccccgtggt gaggacgacg
    14761 aggcgctcga cgacgttttc gagttcgcgg atgttgcccg gccagtcgta ctcctgtagg
    14821 gcttcgagga gggcggggtc tagttcggcg ccggcgcggt tgtgcttctc ggcggccttc
    14881 gcggcgaagt gtgcggctaa gagcggcacg tcctcgcgcc gctcgcgcaa agggggcagg
    14941 tgcagcggca cgacgttgag gcggtagaag aggtcgcggc ggaagcgccc ggcctcgacc
    15001 tcttcttcga ggtcgaggtt gctggcggcg acgagtcgga tgtctacttc gattgtcttc
    15061 gtgccgccga ggcgctcgaa ggcgcgctcc tgaagaacgc gcaggagctt gacctgcatc
    15121 gaggggttga gctcgccgat ctcgtctaaa aagagcgtgc ccgtgtcggc cagctcgaag
    15181 cggcccttgc gcgcggcatg cgcgccggtg aacgccccgc gctcgtggcc gaacagctcc
    15241 gactcgatga gcgcctcggg cagcgccgcg cagctcacgg cgatgaacgg ccccaccccg
    15301 cgcgcgctct cctcgtggat ggcccgcgcg ataatttcct tgcccgtgcc gttttcgccc
    15361 gtgatgagca cggtggcgtt cgtgtcggcg acggcgcgcg cctgcttgag aattttttcg
    15421 agcgcgggcg cgcgcccgac gaagcgcccg aagccgcgaa acatgtccag ctccgagcgc
    15481 agccgcagca cttcctcttc gagacgacgg acgcgcgacg cctgcgcgac gatgtgtttc
    15541 agctccgtct cgtcgaaggg cttttcaaag aagccgtagg cgccgcgacg cacggcctcg
    15601 agcgcggcct gcttcttgtc gctgcccgtg atgacgacga cgggaacacc gggacgcatc
    15661 gcccgcgccg cctcgaccac ggccagcccc tccgtgatcc ccgagaggtc gggcggcagg
    15721 tgcagatcgc agagcaccac gtccacgccc ccgcgcccga ggtgctcgac ggcttcgagg
    15781 cgcgtctcgg cttcgaggac gcggtgcgtg tcggcgagcg cccagttgag ctggcggcgc
    15841 agcacgcggt cgtcgtccac gacgagcacc gtcgcctttt catcggctgg ctctgtcatc
    15901 ggtcggctcg gttcggtctg ggtttgtttc tcggaaagcc tgtcaggccc tgagggggtg
    15961 tcacgcgtcg cgctgcccgt ccggcgtcgc atcggtcgcg tcggcggacg aggccgtcct
    16021 ctttctggcc tgtcccgatt gtgcgtgcgg cgtgaacagc gcggatggta gcacgacgcg
    16081 gaagcgcgtg ccagcgccga cgtttgattc gacttcgagg cggccgccgt gcgtctcgac
    16141 gacttcgcgg caagtgtaga ggccgagtcc gatgcccttc gtcttcgtcg tcgagaaggg
    16201 ccggaacaga cgcgtgcgga tgaactcttc gctcatcccc gcgcccgtgt cggccacgct
    16261 gaagaagacg aggtcgccag cgagcgcgcc cgcctcgacc gtgaggcgcc cgccgcgcgc
    16321 gcccatcgct tcgagcgcgt tgacgacgag gttctcgacg acgttctcga cgcgctcggc
    16381 atcgacggtg gcgatcagct cggcggggag acgcttgtcg atctcgtaga gcggcgcgga
    16441 gggttcggcg tgcgcggcga gcacgcggcg gatgacggcg acgaggtctg tgggacgggc
    16501 gtcgcggcgg tactcgccgc tcatcgactt caccggctcg ctcagtcgcg cgacgatgcg
    16561 gcgcagcttg tcggtcgcct cgcgcagtga ggagacggcg tcggcgcgaa acccctcgtg
    16621 gtcgtagaac cgttccatgt tcgacaccag catcgagagg ccggtgatgg agttcttgag
    16681 gtcgtgcgtg agcatcgcgg acagacgcat gaacgactcg aactgccgcg tctcggcctc
    16741 gcgccggagg tgctcgcgca ggcgctcggt catcaggttg aagccctcgg cgagcacgcg
    16801 cgtcgcgccg ctcgaatcga cctccacccg gcgttcgagg tcgccgcccg cgacggccgc
    16861 cgcgccctcg gcgacgcgct cgatgcgccg gcgggcgcgc gtcgtcaaca cgaagacgag
    16921 cgcgagcgcc accgcccccg cgagcagggg gaggacaatg ccggcccacg ccgcgcgccg
    16981 cggcccggcg acggcgcgcg tgtagttttc cgccgccacg agttcgacgc ccagtccctc
    17041 gggccgcgcc agcgcggcca gccaacgctc gccccccggc gtctcgtaga acgcggtcgc
    17101 gccggctgcc gcctcgcgcc tcaccgagtc gaagtggggc acggcctcga cggccgtctg
    17161 ccccgtgagc aactcgttcg tgtggtagac gacgaggccc gaatcggtgt ccacggcgaa
    17221 gaggacgcgc tcggtcgccc gcgacgtatc atccgcggcc gtcgcgcttt cttcagtggc
    17281 gtcttcaagc agcgccgtca gttttgtctc aaggacgagc gcgccggccg ggcgcgcggg
    17341 cgcgtcctcc gggccgaaga tcgggacggt cgagcgcagg ccgctgccgt aaggctcgcg
    17401 cgacggcgcg cgcagaggct ctttgtgcgc cgtcgtgagc gaccacacgc gctcgtcgaa
    17461 gcggagcgtg ctggtgatga agtccttcgt ctgaaatgtc gtccccgtcg cgggtgggcc
    17521 ggaggtcggg gcgcttgcgc ggaacagcgg gcggccgcgt ccgtccagac aggagacggc
    17581 cgccagccga gacgtgccgg caaatagcgc cgcgacggcc gcgcgcacct cctcgggcgg
    17641 ctccgcggcc tccggcgccg tcgtggtcga gtcgagcacg tatttgcgga gcgcgcttag
    17701 gccggcgacg tgtgtgaggt gcgcctcgtg cgcgcgcagc gtggcggtgg ccctccgcgc
    17761 ggcggcgtcg gcgcgtgcgc gcacgtcttc gcggagcaat gcttcgacgg cgcgcgcgct
    17821 cgccgtgtag ttgtagacgc caatcgccgt cgccggcacg atggcgagca gggcgacggc
    17881 ggcgagcagc ttggcccgga gacccacgag attttgtccc tgcgatggat gaaggcgcgg
    17941 acctcgcggc acgttcacgc gccgcgagtg aatccaagtg cggcagggat gctaacgcct
    18001 gcgtgcggag gctgtcaaaa agggcgcggc cccgagcggc gtgcgctggc cgtcgtctta
    18061 cagactgccg gccttgcgtt ccatgtcgcg ccgccacagt tgtttctgcg tgcgatagac
    18121 gagcacgcac agggcggcga gcgagccggc ggcgacgtag ggcagcgccg ccttcatcgg
    18181 tcgcggcccc gcggcgacga gcagcgcgtt gatgacgacg agggcggcgc gaaactccgt
    18241 cggcccgagt ttcatgtggc tgatctcaaa acgctcggtg gccgcgaagg tgaggaagga
    18301 gttgaccatg aaggctccgt agactgcgaa gaccagcagc aggctgagga gcgcgcgctc
    18361 aggcagcacc agcgcgtagc cgacgacgac cgcgcacagg aaggcgtagt cgagcaggtg
    18421 atccatgtag aagccccact tgaccagtcc cgtatcgcgg tgcttgccga ccttgccgtc
    18481 gaagaagtcc gtcaggtact ggcacaggat catcagcgag acggcccaca tccagcgcag
    18541 gtcggtcgtg gcggcgagac ggctgaagaa gacgatgccg ccgcaccaga gaagcgtgag
    18601 caaggtcagg tggtgcgtct ccagccacgc cgggatgcgc ggcaccacgg cccgcgccat
    18661 gcgccgctcc agcggcgaga cgagcgacac gctgctcttg acggccccgg cgaattgttc
    18721 cctttgcatt tttgaatcct ctataaaaaa aatcggcgag gtgaatcttc acctcgccgc
    18781 tgtcattagc aacacggatg ccagccccgt ttgcccggaa attcaacgct ttgccggggc
    18841 gggtgccata tttacctgac gtttagcacg tcgtcgcgcg ccgtcaatga accgccggga
    18901 gccaacttac ctactgctca ccggcgggtt cggcctgtag gtcaactccg ggttgagccg
    18961 gaggacgatg cgttccatgt gagcgatggt gcccgcgggg agtttctgcg cgagtcgcat
    19021 cgcgccctcc ggccccagca cgcgcctctg gggcggcccc ttctccagct ccgcgtaatt
    19081 gtcgtgcagc gtcacgatga cgtactcgtt ggcgtcgccg ccgagcgtgg tgcggtgaaa
    19141 ccagtagccc ttcacgtccg actttttgac gacggggagg acttcgttct tgatgtgatt
    19201 ctcgtaatcc gcgttgcggc ccggcgtgac actgacgaag gcgaccaccg ccatcttcgg
    19261 cgcttctttc atgtccggcg catggctcat ttcgggatac ccctcgatga tggtggtgcg
    19321 gacgctgttg accatccggc ccgccttggc gagccacgcc gcgtatgcct cggggctgcc
    19381 gagtcccttc gcgacggggt cggggccgtc gaagtccgcg aacttctcca cgggcctgac
    19441 gatgtaatag gtgaagcggt cgccgaaggt ggccgtgttc cacacgtcgc gccacttcac
    19501 gccggccttc gcaagcgccg ggttggtctc gttcttcatc atcgcatcga actcggccac
    19561 catctccggc ttcacgctca cgaccatcaa ggcgagcctt tcacggggcg gcggcggagg
    19621 cggtgcggcc gttggggtct gtgcggcggc cactcgcacc gaggcggcga caagcagaaa
    19681 caggacggcg gcgcacgccg ccgcgttgac gaatctttgc acggttgctc tcatcggttt
    19741 tccctccgtt gtgtcgggtg ttgcaggaga aagcgacggg gctatcaaac cccgaaggtc
    19801 gtctgtaccg taatccctcg ttggttgaac gccttgatcg aagagtgcgg acggccacgg
    19861 ccatccgcgg acggcgcgcg ggcgcgtccg aagagttttt atggtgagaa gttaaagacg
    19921 ggcgccggcg gcggcggccc gtgttgagtg ggcttttatt tcaaagtcgg ggtgcgtgtc
    19981 aatagcggac ggggcgtttt tcagggcgcg gcggcggccg gcgtgaagag tttgaggccg
    20041 acgatgccgg cgacgatgag gccgatgcag gcgagacgca acgcctcgcg cgactcgccg
    20101 aaaagaatca tcccggcggc ggccgtgccg accgcgccga tgcctgtcca gaccgcgtag
    20161 gccgtcccga cggggaggct ccgcattgcc gccgagagga taaagaagtc gatgagcatg
    20221 acggcgagcg tgaagacgct cggccacaga cgcgtccagc cgtcggtgta cttcaggccg
    20281 attgcccaga cgatttccag cacgccggcg atgatgagca gagtccacgc catcggaaat
    20341 tccctcccct gccgagtgct tcgctctcac acgcgcgcgc ggccgaaccc ggccagcagc
    20401 ggcgcaagcg tcgcgactat cgactcgtgc gccgcgccgt tcgtcgcgag gatgccgttg
    20461 cggttgagcg tgtcgggccg gttgtagacg agcggctcgc cccagaggtc ggtcatccgt
    20521 ccgccggcct ctcggagcag cgcctcgggc gcgcacgtgt cccactcctt cgtccgcggg
    20581 ctgagatgga tgtagaggtc gcactgccgc tcgacgagca ggcccacctt gatgccgacc
    20641 gagtggcgct tcacttctgc gctgacgccg agcgcgcgca cccccgcgtc catgcgcggc
    20701 ccgcggtgcg aacggctctc ggcgattctc atgcgcgcga cggtcttttc gtcggagacg
    20761 cgcaggcgct ccgcgtcaag accgggccgc ttgacccacg cccccacgcc gggcgccgcc
    20821 cagtagagca cgtccgtggc gggcgcgtag acgacgccga gcaccgcgcg cccttcgacg
    20881 gcgaggccga tctggacggc gaagtcgccg ctgccggcga tgaacccacg cgtcccgtcg
    20941 agcggatcga ccatccacgc gcgcgcgcgc gcgagacgcc ggtcagtgtc cacggactct
    21001 tcggcgagga tgccgtcgtc ggggaaggcg cgcgcaagcc ccgtgacgat cagctcgttg
    21061 acggcgcggt cggccgccgt caccggctcg tccgtgtcgc ccttgtgctc gacccgcagc
    21121 ggccccccgt agtattcgag cgccgccgcc cccgaggcgc gcgccagctc cagcgccaca
    21181 cccagctcgc gctcgtaggc gttctcctcc accggctggc gtttcgctat catgcaaaca
    21241 aaattaacat ggatggacag aaaggacagg ataagagatt tgaaatctca aatctgtaat
    21301 cagctctttc ttcatcctcc ctatcctgcc catccatgtt aattatttcc ctccccttgg
    21361 ttaagctcgc gcgcgacgcg gcgcactacg tcgagcgtca ggtcgatgtc gcggcgcgtg
    21421 gtgcggaagt tggtgatcga ggcgcgcagg gcgaagcggc cgcgcaggga agcgtttgac
    21481 aggtacgcct cgccgccgcg ctggacgcgt tgcatgacgc gctcgttcaa atcgtcgagc
    21541 cgggcgttca cacttgcgcg cgcggccgac tcggttgccg cgtcgagcgc gcggcgcgcg
    21601 tccgcgctca cgtatctgaa acagcagatg ccgagtgtga ccggcgcgag cagctcgaag
    21661 tcttcggcca tgccgacgcg ttcggccatg tattccgcga gcgcgcagtc ttcggcaatc
    21721 gccgcgccga tgcgcgccac gccgtagtag cgcagcatgg cccagacctt gagcgcgcgg
    21781 aaggggcgcg agagttcgag gccgtagtcc cagaaggcga acgcctcgtc gtcctgttgc
    21841 tcgtagacct tgatgtactg ctcctcggtg ccggcgaagg cggcgcgcgc tcgctcgggg
    21901 tagcgcagga tgagacagcc gcagtcgagc ggcgcgtaga gccacttgtg cgcgtcgagc
    21961 gagacggagt cggcctcggc gagtccgtcg aagagtgcgc gcttcgtcgg gtcgagacga
    22021 gcaagcgcgc cgtaggcgcc gtcaacgtga aaccagagct ggtgctcgcg ggcaacggcg
    22081 gccacctccg cgagcggatc gaccgcgccc gtgttcaccg tccccgcgct cgccaccacg
    22141 cagaagggcc gcagcccgac gcgcgcgtca cgctcgatac tttcgcgcaa actccgcacg
    22201 tccattcgga aattgtcatc ggacgcgacg acacgcacct gctcgcgccc gatgccgagg
    22261 atgtccgcgg ccttcgggat ggacaggtgc acctgatcgg agacgtaaat ggtggcgggc
    22321 gctccggcgt tccacagtcc tttttgcgac aggtcagcgg cgtcgcccgc gcctgtcgag
    22381 ttgcgcgccg cgtgcgcgcg gtgcgcgacg aagagggccg tcaggttggc ggccgagccg
    22441 ccgctcgtga gcagaccgcc gcagctcttt tcggcgtccg cgtagccgat catctcggcg
    22501 agccagcgca ccgtcgtgcg ctcgacgacg gtggccgccg gggccgagcg ccacgcggtg
    22561 acgttctggt tgagcgcgga ggcgagcagg ttggcgaatg cgccgacggg cgcggccggc
    22621 gaggcgacgt agccgaacat gcgcgggtgg ccgttgtggc ggctcgcgtc gaagatggcg
    22681 cggcactcgt cgagcacgcg tgcgagcgtg tcgccctcga cgggcagctc agcgcccgcg
    22741 aggcgcgcaa gactttcggc cggcgcttcg gggaagacgg gcagctcggc gattcgcgcg
    22801 aagtaggcgg cggccatctc gcaggcgcgc gccgacagct cccgcagctc ctcgcccgac
    22861 acgtccagcg acgcggactt agcgcgcccg tcgatgaagt ggctcatcgc ttatgtttcc
    22921 cggccttgaa aagtctccgc aagttcgttt cttcaaacct accacaccac ttgagaaatc
    22981 tgtaggggca gggcaagtcc gcggcctgtg cttacggagg aattaacatg gatggacagg
    23041 acagacagga tagataaaga ctgactttca gatttcagat ttcaaatctt ccgatcctgc
    23101 atatcctgtc catccatgtt aatgattctt tcgccgagac gggccgcgga cttgcctgcc
    23161 gctacaacat tgccgcttga tgcgccgtgt gcgggacggg tatagttcgc gacagtcaag
    23221 tcaaacacag ttcgcaagga gaaccaaagg gtatgtccct cgaaggccgg gccacgcaag
    23281 aggggacggc gcgctatcgc gaccgcttcc ccatcgccag cgccgagcaa cacttccgct
    23341 tcgagcaggg cctatggctc tcctccgtcg gcgtgggcac ctatctcggc aaggccaacg
    23401 aggagaccga ccgcgcctac gccgaggcgg ccgcgcgcgc cgtcgagctg ggcgccaacg
    23461 tcatcgacac ggcggccaac taccgcttcc agcgcagcga acgctccatc ggcgcggccc
    23521 tcgcgcgtct gacctccgac ggcggcttca gtcgcgacga gatcgtcatc tgcacgaagg
    23581 gcggctactt ccccttcgac ggcgagccgc cgcgcgacgt gcgctcctac gtcgaggaga
    23641 ccttcgtccg caccggcatc atcgacttct ccgacatcgc cgccggcagc cactgcatga
    23701 cgcccgccta cctgcgtaat caggtcgccc agtcgctccg caacatgggt ctggggacga
    23761 ttgacgttta ctacatccac aaccccgaga cgcagctctc gctggtgacg cgcgccgagt
    23821 tcgagacgcg cctgagcgcg gccttcgcgg cgctcgaagg cgaacgcgcc gccggtcgcg
    23881 tcggctacta cggcgtggcg acgtggaacg gcttccgcgc caagcagggc gcgcgcgact
    23941 atcactcgtt ggagcgcatg gtggaactcg cccgcgaggc cggcggcgcg gacacgcacg
    24001 gcttccgctt catccagctc cccttcaacc tctccatgcc ggaggcgctc gtcgcggaca
    24061 accagacggt cgagggcgcg cgcatgtcca cgctcgaagc ggcgcgggcg ctcggcgtca
    24121 ccgccatcgc cagcgcctcg atcatgcagg gcaaggtggc gcgcgacctc cccgagcacg
    24181 tccgcgagcc gctcggctcg ctctccaccg acgcgcagac cgccatccag ttcgtccgct
    24241 ccacgcccgg catcacgacg gcgctcgtcg gcatgagccg ccgcgagcac gtcgaagaga
    24301 acctgcaact ggcgcgcgtc gcgcccgcct cggccgacga ctaccggcgc ctgttcgagc
    24361 agggcggcga gtagcgaacc gcgtccgtcg cttcctcttt aatcattcgg aacatttcca
    24421 gccgacgcgt tgtctaacct cattcgagta gcttgtctat agcaattatc agttgagtgg
    24481 gtaataaggg agactcaccg cagagtcgca gagaacgcgg aggggcgcgg agacttgaac
    24541 aggctccctc cgcgtgaact ccgcgtcctt tgcgcctctt gcggtgaatc tacttccctt
    24601 tcactcaagt gaacatcacc ctaacgtctc cgcacaagaa gatgctcgcc gtaaaagaat
    24661 tgacgaagac ctaccccagc ggcgtccgcg cgctcaaggg cgtcacactc gaagtgcccg
    24721 ccgggatgtt cgggctgctc gggccgaacg gggcgggcaa gacgaccctg atgaaaatag
    24781 ccgcgacgct gctcgaaccc gactcaggcg gagtcgagat cgacggcgtt gacctcatcg
    24841 cccataagaa gtcggcgcgc cggatgctcg ggtatctgcc gcaggagttc ggactctacc
    24901 cgaccctgac cgccgagcag acgctccact acctcgccaa gctcaagggc gtcctcgatg
    24961 acaaagaacg ccgggcgctc atcgaggcgc tgctcgaaag agtcaacctc tcgtcggcgc
    25021 gcgcccagaa ggttggcggg ttctcaggcg gcatgcggca gcgcctcggc atcgcgcagg
    25081 cgctcatcgg ccagccccga atgattatcg tggacgagcc gaccgccggg ttagaccccg
    25141 aagagcgcgt ccgcttccac aacctgctct cggaaatcgc cggcgagaac acggtggtca
    25201 ttctttccac tcacatcgtc tccgacgtgt cgaacctttg cgggcggatg gcgattatcc
    25261 ggcagggcga gatcatcgcc tcctgcacgc cgcgggaggc gatgggtcga atcacgggcg
    25321 ccatctggga ggccacgctg ccgcgcgaaa aagttttggc cctgaagaag tcgcggctca
    25381 ggatcgtctc ttcgcagatg ttcgagggac gcgtgcgcgt cagggcttac tcgaaggacg
    25441 agcggccggg cgaggagttc agtcccgcgc tcccgtctct cgaagattac tacttcagcg
    25501 tcgccggcca gccggagagt gcgaattgag aagcccgacg cgcaccgctt tcggcgccat
    25561 cttccgcaac gaggttttgc tcaactcgaa gcgtgtcgct ccttacgcga tggccgcgct
    25621 gttcggcggt aacgcgctcc tgtggtgggg ctgggggccg gccgcgagcc gcggcctggc
    25681 gacgaacagc gatcatttca tcgccgagat gatgccggtc ttctccttca tgacgctgcc
    25741 gctgttcacc gccgtcatca tgggagaccc ggtcatcagg gatttccgcg ccggcgtcac
    25801 gccgctgatt ttttccaagc ccgtcggccg cgccacctac ctgctcggaa aattcttcgg
    25861 caacttcttc gtgctcgtct gctgccagct ggttttcccg ctgacgttct tcctgtttca
    25921 atgggttccc aagtcggggc tgctcgtcca gccgccgcgc gtcctgccct acttcaaaca
    25981 cttcctgatt ctgatcgtcg tcacacatct ggttctcgcg gccgtttact tcaccgtcgg
    26041 cacgctcacg cgcaacgcca aaatcgtcta cggtctggcc gtctcttttt acccgctcta
    26101 catttcctac cagacgctct tattgaggag cctgccgacg cgctggcgca tagctctcga
    26161 ccctctgttg atgaacctca gcttgaacgc gcggggccgc agcgcagagt ttctcaacca
    26221 gttggcggtc agctacgact cggacgtgtt ggcgaatcgc gcggcgatga ttctcgccgc
    26281 cggcctcttc ctgacgttcc tttacgtccg gttttcaatg gtcgagcgcg tcaggaagga
    26341 agaggatgac ggccgactca cgaccatcga cctcagaccc gaccgcgtcg tctccggggt
    26401 ggagagttcc agctttgacc ggccggctca ggtcgaagaa gttattcgcg gggaaagggt
    26461 ggcgatcccc aaagtcggga cggcgagcga aggcgtcggc gcgagtctcg aacagttgac
    26521 ggcagctctg ggaatcgagc tgcgtctgct ctccgccgag cgcggcctcg tcgtgctcgt
    26581 gcctaccgcc acgctcctgt gtgtcgccgg gctggcgtat ttcgaagccg tgcccggcga
    26641 gacgcacacc gccgcttacg cggcccgcac ggcggagtcg gtgctgctgt ttctggccgc
    26701 catcgcggtc ttcttcacgg gcgaggcgac ggcccgcgac cgtgagttga gagtcgagcc
    26761 tgtactgtgg agcgcgcccg cccccaactt cgtgctgctg ctctcgaagt tctcggccac
    26821 gcttctgctc tgcgtcgcct tcagcctggt ggtcggcctc gcagccatcg cgctgcaaat
    26881 cttccggggc cacacgccgt ttgaaatctc cgcgtatctc aaggcgtact cgttgatact
    26941 gattcccagc atcgtcttcc tgacggcggc ggccgtcgcg ttgaacgtcc tgctgcgcga
    27001 caagtacgtt gcctacgcga tgagcttcgc cgtcggcggc gcgctgcttt atctattcaa
    27061 taacggctac aaccactggc tctacaaccc cgtgctttac gggctttgga ctcccgccga
    27121 tttaacagac gccgcgagta atcagacact aatcatcaca caccggattt actgtctcgc
    27181 gatggcgtcc ctgctgctct cgctcgcgca cctctgcttc gagcgcaaat cgacaaaagg
    27241 actgcgctcg cagggtcgtc tgagcggcgc gggatggtca cttttgataa tgtcgctttc
    27301 aactgtgacg gccgtcatca cggggttgat gattaacgtc tgagttgata gtgtgtcgct
    27361 tgaaggccgc tcgctcgcgg cggcatgacg cggcgttaga cacttccggg gctgtgttga
    27421 atccgacgga cgatttttat tgtgacgagg cgttgagcgg gcgcacgccc gtgaggaagg
    27481 tcgccgagac ggacaacgtg ctcgccttcc accacacgcg ccccttctac ccggcgcacg
    27541 tcgtcgtcat ccccaaacgt cacatcgcgt ctctcctcgc cctcgacgcg gaggacgacg
    27601 aacttctgct tgaactgctc gcggtcgtcc ggcgcgtcgc cgccgaggtt ctggccgaac
    27661 acggcgcgtg tcgcgtcatc accaatctcg gcgagtatca agactccaag cacctgcact
    27721 ggcacgtcgt ttcgggccaa ccattgagat gacgcggcgc gcgcgcaaag acgaagcagc
    27781 ccgcgcgcgt ctgtttggaa cgcgcgcggg ctgctttgtg cgaagagtga tgtaggtggt
    27841 agttagttac ggccgcggcc gcgacggcga ttgtccgacc cgaagacggg gtggttgcgc
    27901 gagccggcgc cgcggcggta gtagccgttg ccgcggcgat gattattgcg gcggttgtcg
    27961 tagccgtcgc ggcggtcgtc gttgtggcgg gcgcggcgga tctggcggtc gtgctggcgc
    28021 tcgtcgcggc ggatgtcgcg gcgctcctcg cgcgtgatgg tgccgtccga gcggtagccg
    28081 cgtcgctccg tccgattctg gcgctggcgc tcgcgcaaga cccgcgcctc gtcgcgtgtg
    28141 atctcgccgg agcggacgcc gcggcggatg cgttgacgat cattgcgctg gccgaacgtg
    28201 tcggcggccc ccaggccgaa gaacatgacg atggcgccaa gaagaagtgc ttttttcatc
    28261 ggttaaaccc tttcctcaag aagtgtgatt ttgtaaactc gtggcattta cgtttaactg
    28321 ctgcctgcac accgccggag agagttctgg agtgtgattt cacctcggag cacggcaact
    28381 accatgcccg gcccggaggg tcgccgtatt ggcccgtaag ggttgatagt tcaagctccg
    28441 tccgtccgtt ttaaatgtcc ggccgcgggg cgcggctgct aaaatcgaac gcccggtgca
    28501 tggtttccgg cggcgggtgg cgaggtattt ttccgggggc gataacagta ccgcgagcgg
    28561 tagcgagcgg gtgcggcata caatggcagg cccgcaccgc tcacgcgaga cccgcgaggc
    28621 ttccgctcgc ggtactgttc acaagtctgc ggggcggggg ttgaatcaag catgtcacag
    28681 cgtgagacaa ggacgttcgc ggagttctgg cccttctacg tgatggagca cagccggccg
    28741 ggcacgcgct tgctgcacgc ggccggcacg ctcgggagca cgacgcttct ggtcgccctc
    28801 atcgcgacgg ggcgctggcg ctggctgccg ctggtgctcg tcgtcggcta cggcgcggcg
    28861 tgggtcggcc acttcttcgt cgagcacaac cgccccgcca ccttcgcgca cccgctctgg
    28921 tctttcgcgg ccgactacaa gatggtctgg atgatgctca ccggccggat gagcgccgag
    28981 gtcgagcggt cgcgcgccgt cagggccgcg gcacgacaat cctgatctcg ccgacggcgc
    29041 ccgtcgccgg cacgttggct atgtttgagt tgacccaact gccgtccgca cgattgacgc
    29101 tcacgctcag cttcatcccg ataccttcgt acaccttgaa cgagaagcgg cccgcctcgt
    29161 ccgccgtcgc ggcgtccccg atgagcatgt tcgggatgtc ggacgtgtag ctgatggtga
    29221 cgccggcggc cggttggccg tccggccaga cgacgacgcc gctgaccacg cgctcgccgg
    29281 gcagcggcgg cagtcgtatg tcgtagtcct cgacgtgctc gccctcactt atctggacga
    29341 cgcgggcgcg cgccgcctcc gtcacgttct cgtgatagag gacggggaag gggcggctcg
    29401 cggtcggctc gccgtcgaag cggatgcgga tgatgtagcg gccgggcggt atacccttaa
    29461 cctcgtagcg gccctccgcg tcggtcgtcg cgtagccgac ggggccgcgc aaggactgct
    29521 cgccggactc ggccgcgctc agttgcagcg acaccctgga ggccgggcgt cccgcggcgt
    29581 cgaacacgcg cccgctcagt cgcccgttct catcggcgta gaacgacgcg acggcgcacc
    29641 cgcgctcatc gatcctcacc ttgcgctccg gctcattcac catcagcgtc ttcggcaagt
    29701 cgagcttcaa cacgtactcg cccggcggca acccaccgaa gcgatacttc ccttccgcgt
    29761 ccgtctcggc gcgaaacgtc ttgcccttgc ccgcggcctc gattttcgcc ccggagacgg
    29821 ggacgctggc gtctcgttcg ctcccctcat cgtccttctc ccgacgcgcc ctcaacacct
    29881 gcccgtagat ggcgccgccg gcgggcggcc cctgcgagag gccgtcgatg tagcggaggt
    29941 cttcgccggc ctcggcgacg ggctgcgtgc gggagcagat gcccgtgtag agccgctgcg
    30001 tcttctcgtc gcggtacgcg tagatgatgt agcgcccgcc gcgctcgaag ccgtaaccgc
    30061 agtcgccgcc gcccccgccc gtgagcactt cgacgtaggc gtcagccaca ccgcgcaacg
    30121 gccgctccac ggcgaagcgg aacgcgcgcc acccctgttc gtgcttgttc tctccttcgc
    30181 cctcctccac cctcacgttc gagaccccga cgacctgccc gacgaagacg gccgacgccc
    30241 gccagtaagc ctcacacggc ggggcgctgc ccgcgcacga acaggcgcgc gcctcgactg
    30301 agaagacgac gagcgtgagc agaaggaacg agaacagttt gagcgcggtg ttcatctttg
    30361 gcctcatgtc gcctgtcgcc ggcctacttg ccttcgagct tcttcaacat ctggacgccg
    30421 ttatcgttct tcggattcag ctccagcgac tttttgtagc tggcgatggc gagttcgcgc
    30481 tccccgttct ccatgtaggc ttcgccgagg ctgtcgtagg tgttgaacga ctgcgggtag
    30541 aactcgacgt tgagcttgaa gacggcgatg gcttccgcca gcttcttttg tcgcatcagc
    30601 tcatagccga gcaggttgag gcgttcttct ctgacggcga cgttggcggg ctgttccttc
    30661 ctgatttgcc ggtatccggc cagcgcttcg gcggtcttgc ccgacatcaa caggtcgtaa
    30721 ggaatcaaca cgtcatcgcc gacgcgcgcc gcttcgttcg ccccgccgtc ggacttgacg
    30781 cggatggcgc cggcgcgccc ctcggcgttt ctcacgaagg tgtactgcac gttctggtcg
    30841 cgtctgatga aagtgtcttc ggagatgggc aggagttcga ccttcggccc ctgtgtcggc
    30901 tgggcgtaga ggcgcccccc ctccttcgtc acgttcagca ggcggtcggg gttgatgcgg
    30961 aagcggccga cgtaggcgtc gagcctttcg ggcgcgagcg tcgtcacctc gaagggcgcg
    31021 ctcagatagt cttcccacgc gtactcgcgc gcgattgacc taacgatctc ttccataatc
    31081 tggccgttgt cggaattgac catgacgacc gcgccgtagc ctcgctcgcg gtgcgcgagg
    31141 agcatcgcgc ggaagccctc gtcggcgccg ccgtgtccga agtatgtggc cgcgccgtgc
    31201 ttctggatga agaagcccat gccgacgttc tccgacgcga agggcgtgac catccgctca
    31261 acgctttctt tcgagagcac cttgttggac ttgccggcga gagagagttg cacctcgatg
    31321 gcgaacttcg ccaaatcggt cggcgtcgtc cacagcccgg cggcggccat ctcggggtag
    31381 acgtgaatct tgcccttgac ctcgctgccg tccggcttgt agccggtggc ggcgcgcggc
    31441 cgccagtcgt cggggagcgg ctggctgtat gtgctgttgg tcatctggac ggggccgagc
    31501 accgtctctt tggcgatctg cgggtaggat ttgcgctcaa cgtccatgac ggcgagctgg
    31561 gcgatggtgg tgccgccgcc cgagtagcga aagcgcgtgc ccggctccag atcgacacgg
    31621 acggccgccg tgttggcggg cgcggcgccg tcgaggacct gcggaagcgt cgggagcttt
    31681 tcgctgacgg cgtagccggg gaagccgtgg acggtcgtcc ccgcgctgtg gctcaggagg
    31741 ttggcgagcg tcaccttctt cttcgcggtg aactcgttgt cgggcagctt ccaggaggtc
    31801 agacgattat tgatgttctc atctaacgcg agcttgccct cctgcacctt gcgcaacgcg
    31861 accatcgccg ccacgctctt gctgatcgag ccggcctgaa agagcgtctc ggcggtggcc
    31921 gcctccttcg tcccgacatc cttgaggccg taggcgcgcg cccactcgac cttgaaatct
    31981 ttgaagacgg cgacgctgac ggccggcgcc ttgtagtggc gcatccgctc ctcgattgac
    32041 cacgtcgggt cgcccttgat gaccaccgcc gtcaggagac ccttctccac gcggccgatg
    32101 cgcgactcga ccccgccgac gctctgcccg aggctgacga cgtggacgac gcagacgagc
    32161 aggagactaa cgatagcttt tggtctcata aatcctctca ggtgtgggcg aaagttgaat
    32221 ttgtttccga cgggagatta acgcccggcg tgcctgacaa gttgccacat tctagccgcc
    32281 tgatttccgt agcgtcaaaa ttatagtagt gtcccgattc gggtat
//
LOCUS       DASVES010000324         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1381187, whole genome shotgun sequence.
ACCESSION   DASVES010000324 DASVES010000000
VERSION     DASVES010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381187"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1085)
                     /locus_tag="VF240_13415"
     CDS             complement(54..1085)
                     /locus_tag="VF240_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176255.1"
                     /translation="MPVFRSLCLRAPLNVPLTALLCLVAFTPPTSAQELDQYGRWVNG
                     VSEPWFFGAELDKAEVSAAQTRWKLIEEENGRAAGGEWAGDYFVGDETHGSFLRWAPG
                     AGFVLFKVNKCAAQVDDFSYGRVVVSPTLVMLVPERAMRPRGAHGHGHHPSGNHYVPV
                     KFRGERLLIPEGEMSDFGDYVAGLGQFNRHQFFSYIMTTSFFSQFGGSEAEPAAAPVV
                     PPGYERFLKRPLTGRVVSVGRRSIRRDYGYENEDGTGGVYNGLSSLARLTVNVGRAHG
                     AKRGMILRALATGEDIRLTRVGKLTSEGLVVRSLGDNGQETYFDHETNRDKVYPTVVV
                     GWRLTTSPF"
     gene            complement(1195..>2165)
                     /locus_tag="VF240_13420"
     CDS             complement(1195..>2165)
                     /locus_tag="VF240_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7176256.1"
                     /translation="LKMYAANEEGVWNASVEFDERTLQPTYRLLVGVAGSSSGLDIAR
                     RFGIPEAVITAARARVDEQTLAASDYLRRIQREAEEAENLRRALEEERRAAELRREAA
                     QKRSATARTATAGGGTIEGGGDAATPTTAGGVRVVRQGREVGKSKAGAAGEGDELRVV
                     ERAPVAGDRVRLKTLGATGVVERISDGEAEVRVGAVRFRERVANLEVVEGQTRKRDDT
                     LAGRLQRMAAGGPEVKLRDAGARADAELNLIGRTTDEAADAVDKFLDEVYLEGHTHVR
                     VIHGHGTGALRRAVAELLKNHPHVASFGPAPANQGGAGATVVELKQ"
BASE COUNT          311 a          757 c          754 g          343 t
ORIGIN      
        1 gaaagtttga ggaaatcgca tgggcggctg tagatgtcct ctgccacgac gttttagaac
       61 ggtgaggtcg ttagtctcca gccgaccacg actgtcggat agaccttgtc tcggttggtc
      121 tcatggtcga agtaagtctc ctggccgttg tcgcccaggc tcctgacaac gaggccctcg
      181 gacgtgagct tgccgacgcg ggtgagacgg atgtcttcgc cggtcgcgag cgccctgagt
      241 atcatcccgc gcttggcgcc gtgggcgcgg cccacgttga cagtgagacg cgcgagggag
      301 gagaggccgt tgtagacgcc acccgtcccg tcctcgttct cgtagccgta gtcgcgcctg
      361 attgagcggc gcccgacgct gacgacgcgt cccgtgagcg ggcgcttcag aaaacgctcg
      421 tagccgggag ggacgacggg ggccgcggcc ggctccgcct cgctcccgcc gaactgtgag
      481 aagaaggagg tggtcataat gtaagagaag aactggtgcc ggttgaactg acccaagccg
      541 gcgacgtagt cgccgaagtc cgacatctcc ccctcgggga tgagcaggcg ctcgcccctg
      601 aacttcacgg ggacgtaatg gttgcccgaa gggtggtggc cgtgcccgtg cgcgccgcgg
      661 ggccgcatgg ccctctcggg cacgagcatg acgagcgtcg gcgagacgac gaccctgccg
      721 tagctgaaat cgtccacctg cgcggcgcac ttgttgacct tgaacaggac gaacccggcg
      781 ccgggcgccc agcgcaaaaa gctcccgtgc gtctcgtcac cgacgaagta gtcgcccgcc
      841 cactcgcctc cggcggcgcg cccgttctcc tcttcgatca gcttccagcg cgtctgcgcg
      901 gccgagacct ctgccttgtc caactccgcg ccgaagaacc acggctcgct gacgccgttc
      961 acccagcggc cgtactgatc cagctcctgc gcggaggtgg ggggcgtgaa ggcgacaagg
     1021 cagaggagtg ccgtgagggg cacgttgagc ggcgcgcgca gacacagcga cctgaaaact
     1081 ggcatggcgt gactcctcgg gcaggggtgc ttccttacaa aactatccga ccgcgggcgg
     1141 gggcggcaag cccgagccga cggccgcggc tccttctcgc ccgacatcgc ggccttactg
     1201 cttcaactcg acgacggtcg cgcccgcgcc gccctggtta gcgggggcgg ggccgaagct
     1261 ggcgacatgc gggtggttct tgaggagttc ggcgacggcg cgtctgagcg ccccggtgcc
     1321 gtggccgtgg atgacgcgga cgtgcgtgtg gccttcgaga taaacctcgt cgagaaactt
     1381 gtccaccgcg tcggcggcct cgtcggtggt gcggccgatg aggttgagtt cggcgtcggc
     1441 gcgcgcgccc gcgtcgcgca gtttgacctc ggggccgccg gcggccatgc gctggagccg
     1501 gccggcgagc gtgtcgtcgc gcttgcgcgt ctggccctcg acgacttcga ggttcgcgac
     1561 gcgctcgcgg aagcgcaccg cgccgacccg cacctcggcc tcgccgtccg agatgcgctc
     1621 gacgacgccc gtcgcgccca gcgtctttaa gcgcacgcgg tcgcccgcca cgggcgcgcg
     1681 ctcgacgacg cgcaactcat cgccctcgcc ggccgcgccc gccttcgact tcccgacctc
     1741 gcgcccctgc cggacgacgc ggacgccgcc cgcggtcgtc ggtgtcgccg cgtcgccgcc
     1801 gccctcaatc gtccccccgc cggccgtcgc cgtgcgcgcc gtggccgaac gcttctgagc
     1861 cgcctcgcgc cgcaactcgg cggcgcggcg ctcctcttcg agcgcgcggc gtaagttctc
     1921 ggcctcctcc gcttcgcgct ggatgcgccg caggtagtca gaggcggcga gcgtctgctc
     1981 gtccacgcgc gcgcgcgccg ccgtgatgac cgcttcgggg atgccgaagc ggcgcgcgat
     2041 gtcgaggccc gaagaggagc cggcgacgcc gacgagcaga cggtaggtcg gctgcagggt
     2101 gcgctcgtcg aactcgaccg aagcgttcca gacgccctcc tcgttggcgg cgtacatctt
     2161 gagcc
//
LOCUS       DASVES010000325         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1329934, whole genome shotgun sequence.
ACCESSION   DASVES010000325 DASVES010000000
VERSION     DASVES010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329934"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF240_13425"
     CDS             <1..634
                     /locus_tag="VF240_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176257.1"
                     /translation="GEMIKREPEMLSLVELSVATEEARQGKPLDVAAYRRQMRRALRH
                     ESPHAVEKELRALRELAARSAKAGDWPNAGAIYHAALDEAVRGYDDVLREMDEDGDIS
                     ILIDEFALGLTQCLKKSKADAETRRAWLEALLEAELTDIEMGGIDLAPSARAAVLEHA
                     NDEEWAWIEKRLRTAISKSHDWGREALTRFLAAGEKRHGRAGESKTARKK"
     gene            846..>2672
                     /locus_tag="VF240_13430"
     CDS             846..>2672
                     /locus_tag="VF240_13430"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7176258.1"
                     /translation="MLRFEKAAVLGAGTMGAQIAAHLANAGIPTLLLDIAPREPTPEE
                     QAKGLTLDSKDVRDRIARAGFEAAKKAKPAAFFTPEAAALVSVGNFDDDLARLKDCDL
                     IVEAVVENLDIKRGLYERVEKFRRPGSVVASNTSGIPIRQLAEGRSEDFRAHFLGVHF
                     FNPPRYLHLVELIRTEWTKPEVSCALYGFLDRRLGKGVVVAKDRPNFIANRIGTYGGL
                     VTIKTMLEDGYSVEEVDKMTGQAVGRPKTATFRLFDLVGLDVFMHVAKNLYEAVPEDE
                     DRDAYLAPDFARGMVERGLLGNKTKGGFYRRQKGEGGKQEIWTLDVATLEYRPAEKVK
                     LPALDAARNIEDTRERLRTLTWGKDRAGQFLWKTISKTFVYAANRIPEIADTVLEVDR
                     AMKWGFNWELGVFEAWDAIGVEKSVARLEEEGRKVPENVRRMLEAGAKSFYKSEGGQQ
                     SYFDFATGAYVPVAEQPGVAVLKSVKDRTAVVKKNASASLIDIGDGVACLEFHSKMNS
                     IGGDTLQMVKTALAEVERNFVGLVVGNQGQNFSVGANLMLVLLGAQEEEWDEIELMVR
                     AFQTATMSLRYSAKPVVVAPFGMTFGGGCEMTLHADRVRAAAE"
BASE COUNT          548 a          805 c          888 g          431 t
ORIGIN      
        1 cggcgagatg atcaagcgag agcctgaaat gctatctctg gtcgaactct ctgtcgcaac
       61 tgaagaagct cggcaaggca agccgttgga tgtggcggcg tatcgccgtc agatgcgtcg
      121 ggcgctccga catgaaagcc cgcacgcggt agagaaagaa ttgcgggcgc tcagggaatt
      181 ggccgcccgt tcggctaagg ccggcgactg gccgaatgcc ggcgcgatct atcacgcggc
      241 tctggacgag gcggttcgcg gatacgacga cgtgttgcgg gaaatggacg aggacggcga
      301 catctccatc ctgatagacg agttcgcgct ggggctgacc cagtgtctca agaaaagtaa
      361 ggccgacgcc gagacacgcc gggcgtggct ggaggcgctt ctggaggccg agctaaccga
      421 tattgaaatg ggcggaattg acctcgcgcc gagcgcccgc gcggcggttt tagagcacgc
      481 caatgatgag gagtgggcgt ggattgagaa acgtctgaga accgctatct cgaagagcca
      541 cgactggggg cgagaagcac tgacgaggtt tttagctgct ggggagaagc ggcatgggcg
      601 cgcaggcgaa agcaaaacag cccgaaagaa atgatcaagc aggtagcgaa ccacatcacc
      661 taaaggacac gatgggcagg aatgaaatgg aacaggcaga ccgacagaaa acgctgaagg
      721 cagtcctgca atatttcccc caggacaaca caaggttccc gccctggcct tgctgaatga
      781 atgctcattc agtatgatgg ggccgtcctc gactccctga ctttctcgga aaggcaagtc
      841 cccttatgct ccggttcgaa aaggccgccg tcttaggcgc cggcacgatg ggcgcgcaga
      901 tcgccgcgca cctcgccaac gccggcatcc ccacgctgct gctcgacatc gccccgcgcg
      961 agccgacgcc cgaagagcag gcgaagggac tcaccctcga ctcgaaggac gtgcgcgacc
     1021 gcatcgcgcg cgccggcttc gaggcggcga agaaggccaa gcccgccgcg ttctttacgc
     1081 ccgaggcggc ggcgctggtg agcgtcggca acttcgacga cgacctggcg cggctgaagg
     1141 attgcgacct catcgtcgag gctgtcgtcg agaatctcga cataaagcgc ggtctctacg
     1201 agcgcgtcga aaaattccgg cggcccggct cggtcgtcgc ctcgaacacg agcggcatcc
     1261 ccatccgcca gctcgccgaa gggcgctccg aagacttccg cgcacacttc ctcggcgtac
     1321 acttcttcaa cccgccgcgc tatctgcacc tcgtcgagct gatccgcacg gagtggacga
     1381 agccggaggt gtcgtgcgcc ctctacggct tcctcgaccg gcgtttgggg aagggcgtcg
     1441 tcgtggcgaa ggacaggccc aacttcatcg ccaatcgcat cggcacctac ggcggacttg
     1501 tcaccatcaa gacgatgctc gaagacggct actccgtcga agaggtggac aagatgacgg
     1561 gtcaggcggt gggccggccc aagacggcga ccttccgtct cttcgacctc gtcggcctcg
     1621 acgtgtttat gcacgtcgcg aaaaatctct acgaggccgt ccccgaagac gaagaccgcg
     1681 acgcctatct cgcgcccgac ttcgcgcgcg gcatggtcga gcgggggctt ttggggaaca
     1741 agaccaaggg cggcttctac cgccggcaga agggcgaggg tgggaagcag gaaatctgga
     1801 cgctcgacgt ggccacgctc gaataccgcc ccgccgagaa ggtaaaactc ccggcgctcg
     1861 acgccgccag gaacatcgag gacacgcgcg agcgtttgcg gacgctcacg tggggcaagg
     1921 atcgcgccgg gcagttcctc tggaagacga tctcgaagac gtttgtctac gccgccaacc
     1981 gcatccctga aatcgccgac acggtgctcg aagttgaccg cgcgatgaag tggggcttca
     2041 actgggagct gggcgtcttc gaggcgtggg acgccatcgg cgtggagaag tcggtcgcgc
     2101 ggctcgaaga ggaagggcgt aaggttcctg agaacgtgcg gcggatgctc gaagccggcg
     2161 cgaaatcttt ctacaagtcc gaaggcggcc agcagtctta cttcgacttc gccacgggcg
     2221 cttacgtccc cgtggcggag cagccgggcg tcgcggttct gaagtcggtt aaagaccgca
     2281 cggccgtcgt caagaaaaac gccagcgcgt cgctcatcga catcggcgac ggcgtggcct
     2341 gtctggagtt tcactcgaag atgaactcca tcggcggcga cacgctccag atggtcaaga
     2401 cggcgctcgc ggaagttgag cggaatttcg tcggactcgt ggtcggcaat cagggccaga
     2461 acttctccgt cggcgcaaac ctgatgctcg tcctgctcgg cgcgcaggag gaggagtggg
     2521 acgagatcga gctgatggtg cgcgccttcc agacggcgac gatgtctctg cgctactcgg
     2581 cgaagccggt cgtcgtcgcg cccttcggga tgaccttcgg cggcgggtgc gagatgaccc
     2641 tgcacgccga ccgcgtgagg gctgcggcag ag
//
LOCUS       DASVES010000326        15059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_11194, whole genome shotgun sequence.
ACCESSION   DASVES010000326 DASVES010000000
VERSION     DASVES010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15059
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11194"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF240_13435"
     CDS             <1..258
                     /locus_tag="VF240_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176259.1"
                     /translation="QAFFWSTFGRQLPLSAVGQSATHDRFGYDHQHAVDVPLHPDSLE
                     GRAVISYLQAQGITFTAFRAAIPGVATGPHIHIGRPSNRLS"
     gene            432..2108
                     /locus_tag="VF240_13440"
     CDS             432..2108
                     /locus_tag="VF240_13440"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7176260.1"
                     /translation="MKLQLNRKRRMAVVLVAIVVAGVAGFWYWGDGASAAPYMTGRVE
                     RGSLRNTVTATGTLQAVTTVQVGSQASGTISALLVDFNSMVKKGQIIAQLDPAVSQAQ
                     VQQARANLGQAQAGLEQARAGVVQARAGLADARAKALSAGSTAQGQRAGVSGAEANVA
                     VLKAQLDDAASFMRQQESLVQSGIISQRELETARTSYRTAEARYNQSQAQLNQAVVGE
                     KSASSSGQAQATAQVQNAQAQIVQAEAQVRNAQAQVQQAQAALQLSELNASHTTIRSP
                     IDGVVVSRNVDVGQTVAASLSAPTLFTIANDLTQMQVIANIDQADIGLVEQAKGVKFS
                     VDAFPDKEFEGTIRQLRLNPVSAQNVVTYNVVMDVSNPEQKLKPGMTANLSITIDERQ
                     DVLKVPNAALRFTPQLTEEQRAAAAERRAAGGQGQGGERRARRQQNENTNAGAPDANA
                     TATNAAPADGGRRGDFASATSPVTAGQTRRVWVMGADGQPEPRRIKVGLTDGAATEVV
                     EGDLREGEVVITGQNVTGTQQQNRQQGAQSAPGFGGAPRTGGGGGGGRRN"
     gene            2210..3037
                     /locus_tag="VF240_13445"
     CDS             2210..3037
                     /locus_tag="VF240_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176261.1"
                     /translation="MQTAYEIGRQTFEAGAGVAASRAEQQARPVIELEGIHKTYAMGD
                     VEVHALRGVSLTIREGEFVAIMGTSGSGKSTMMNIVGCLDRPTRGVYTLDAQDVSGMS
                     KDERADVRSSKIGFVFQGFNLLSRTSALENVELPMLYANVETAERKRRAVEALHSVGL
                     AGREQNHPNQLSGGQQQRVAIARSLVNNPTLILADEPTGALDSRTSVEVMEIFQRLNR
                     ERRITLVLVTHEPDIAAYADRVVVFKDGRIQKDYRVDAPRDAAEELINLPEIEPEED"
     gene            3082..4308
                     /locus_tag="VF240_13450"
     CDS             3082..4308
                     /locus_tag="VF240_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7176262.1"
                     /translation="MNALAIIRIAFRALARNKMRAALTMLGIIIGVSAVIAMVSIGQG
                     AQASVQAQIEGMGTNLLFVSAGAQNTGGVRGGAGQDGGNNTLNAEDLEAIRREVPSVS
                     MVSPVVNARSQLVAANLNWNASVQGVSQQYPDIRKWAVQSGEFFTDADVRTAARVVVL
                     GQTVADNLFPGADPVGQTVRVTNLPFRVVGVMARKGQDPQGRDQDDLAFAPYTSVQKK
                     LLGNTRLQIAYVSAVSQDATYTAQAQITDLLRQRHKLAPNEGNDFTVRNMSDVAEAAN
                     ETNSTMTILLGCIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARSSAVRTQFL
                     IESIVLSLTGGVLGIVLGVGVSVSIPAMLGWPTLVSTAAVIGSVVFSAVVGVFFGYYP
                     ARKAAALDPIEALRYE"
     gene            4389..5021
                     /locus_tag="VF240_13455"
     CDS             4389..5021
                     /locus_tag="VF240_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7176263.1"
                     /translation="MTNTEVLKMWPGVETATAPEAAAPESARGRDFELAQRAGAGDAE
                     AFEQLYTLHHRRVYGLCLRMMQNPTEAEDLAQDVFIQLFKKIGMFRGESAFTTWLHRM
                     TVNQVLMHFRKKGVRVEQTTESGEMTEQVVAGTEDHARMPVVDRIALDNAIAKLPPGY
                     RAVFVLHDVEGYDHAEVGRMLGVAAGTSKSQLHKARMKLRGLLKRPRPLR"
     gene            complement(5249..6787)
                     /locus_tag="VF240_13460"
     CDS             complement(5249..6787)
                     /locus_tag="VF240_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176264.1"
                     /translation="MKLAEQLVERMADAALTHGERARLRVQLSRALEESGDYEGARAA
                     MGDLWSRVGEVPAVEGLDRRAAAEVLLQAGVLTSGLGSVKQVEGAQEVAKDLISEAAA
                     IFDALGDVEKTAEAQSDLAVCYWRQGEYDGARAWLREALSRLPDDSEVKAVALLRSAI
                     VETVANRLGDSLRVYTDAAPFFEHVSNHVLRGKFHGGFATTLKNIGMAEHREDYIDRA
                     LMEFTAASFHFEQAGHERYCARVENNLAMLFVEAGRAPEAHEHAARARKMFAKLGDGG
                     SVAQVDETLARVLLAEGRDAEAEGVARAAVRALERGDELSLLAEALTTHGTALARTGR
                     FPQALATLRRAIEVAQGAGDREGAGRAVLVVIEEVGEHLSPEELCDAYASADDLLGKT
                     QHPGTLARLNACARRAVAAFKAVGSGSAAAQGEDGAEVFGLPTDWKGFNFKEEVRRFE
                     SLLIERALKEAGGSVTHASRLLGLKHHHSLSSILKGRHNDLLGARTPALSRRRSIFKI
                     KQDA"
     gene            6966..7208
                     /locus_tag="VF240_13465"
     CDS             6966..7208
                     /locus_tag="VF240_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176265.1"
                     /translation="MKTLRQLSASALLMLVFALPAFAGDMHFPVAPPQPPPAAPAPLT
                     QPNSTSGDAALGVETADVDPLTEITLLMLQGVLALF"
     gene            7969..10257
                     /locus_tag="VF240_13470"
     CDS             7969..10257
                     /locus_tag="VF240_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176266.1"
                     /translation="MDRLVQFLSGHEPAVFSNGQFGFDVRLHPALLAVLALLFAALVY
                     FVYVRARARPPGRRLAGLAALRAALFVLLVFLLMKPVVVVPSVVPRSSSVALLFDDSR
                     SMQQPDGPERRTRLDAIRDTLLSAEGTFLPRLREKFRTDLYAFSGELTGLRGGDELLG
                     EGTASDPGGAIAEAARRATVVPLSAVVLVSDGAANVPSDLAAELRNLRARGLPVYTIG
                     VGSAARQTDAELLRVSVPRRVLVGSSAQVEAFVRLAGYGATKVLIAVSEDGRAIRTEE
                     FSLRGAETEAVRMEIVPTTPGTHRYTFEITPLDGELSVENNRREALIEVVEGPRRVLY
                     VEGEPRWEHGKMRAAVGRNEKQVELVSVLRSGENKLYRQGVAGEQELAAGFPATEEEL
                     FAYHGLVLGSVEASFFTAEQLRAVEAFVARRGAGLLALGGRLAFDGGKYAGTPVADLL
                     PLALDGRAGQPEDLSNPVYKPLLTARGATHPVTRLGEDRALSQKIWNELPPVSVPETF
                     GGVKPGATVLVEARREGGGATVPLLAHQRYGRGQTMALATSDTWRWQMRMDSKSGAHE
                     NFWRQMLRYLVSGSPAQVEVATEQDVYAVGDPVRIVADVRDKKYNAVTGARVAARVVK
                     PSGASVDVPLRYTARDDANVYVGEFRPDELGQHRVELSAAGEGVGEGAAQSEFLVAEL
                     NREFYDAALNEDLLRRIAAETGGKYYTLEEAGALIGDLTYRRTDNSTLVTKDLWDMPV
                     NFVLLIGMVSAEWFLRKREGLA"
     gene            10254..11285
                     /locus_tag="VF240_13475"
     CDS             10254..11285
                     /locus_tag="VF240_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176267.1"
                     /translation="MTRRLIKALICGLMVAGAACGVMPRAQTPAQTVPAARPAADPNK
                     FAVIISGASGEEVYAKQFARWTTELRGVLVGRLGFAEDRLKILAESAGEGTPAARATA
                     EEVRRAFESLRGAAGPESTLFVFFIGHGTFDGKQAKFNLVGPDITAGEYASLLGAVPC
                     RRVVVFNLSSASGEFAKTLAGRGRVVVTATRSGQEQNAPRFAEHFIAALTGRESDADQ
                     NGRVSVLEAFDYAARLTAEHYKRSGRLATEHALLEDSGDGVGHEKAEGGDGDIARATY
                     FDSLTVEQAAANAEVGRLLRERTRYEEEIGQLKTRKREMAEGQYESELERLFVGLAKT
                     NRSIKRGGQ"
     gene            11282..13990
                     /locus_tag="VF240_13480"
     CDS             11282..13990
                     /locus_tag="VF240_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176268.1"
                     /translation="MKQHEQTQNIQRRRSRQSIRPSVVCAVLLAVCALLYSQAPAGSA
                     QSADEALPGLAELKKGDYERAVKVLGARLATNPSDAAARKHLLRAYMETGHYAEAETA
                     ARGFLEKWPEAGGVRHELAESLAATGRYAEAVVEFERAAKDLSKTGVLPAERLESDLR
                     RAEVLQLTGQEERAREIFQSLVTFHEENDTEEDNDAKSAPVLTLVARALAHLEKYREA
                     TELYMAAAEADPSHTEAHLGGGELFTEKYNYADASEFFADALRVNPHSARAHLGIAAN
                     KRLEGGAEVSAALERALKINPNLVAARALKAALDLEAGDFDAAAAEIDKALKVNPRSS
                     EAHALRAALAYLQDRDPLPAAAAALATNPRDGRVYDTLSHYATITRRTAQAADFSRRA
                     VELQPRLWRAHLSLGMALLRLGRMDEGRAAVEKSFEGDPFNVWAKNTLDLLDSMRDFR
                     ETRRGNFIIKADAKETEVLAPYAADLLEEAARELTAKYRFTPQGPVMVELFKNHEDFA
                     VRALGLPGLGALGVCFGPVIAQDSPSARPEGQFNWGSTLWHEYTHVITLQMTDYRIPR
                     WFSEGLSVYEERRARPGWGDEWNPSVLRAFSEGRWFKIAELDSGFMRPRRADDVSLAY
                     FQASQVVEYVVERHGFDAVLRMLALYRDKARTPDVLRQALKLTEAEFDRAFVEYVGGK
                     ARPLIEALGAEGGATAAGMPKDAVLKSLGERENFALHLRAGSLYRAEGDAANAARHLK
                     RAVELFPYYTGEGNAYELMADIFEKQGDTAEAAQALELLVRLDENNLPALKRLADLRL
                     KQNDAARALEALRLGFYVSPFDHAAHARAGELLMARSEAGSALGEFQAALALKPPNEA
                     EANYNVARAYHALGKSAEAKRAVLRALEAAPSYEKAQELLLTITGQ"
     gene            14233..>15059
                     /locus_tag="VF240_13485"
     CDS             14233..>15059
                     /locus_tag="VF240_13485"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7176269.1"
                     /translation="MAVETTVMRDEELLDRLATAREKLLREIRKAVIGQDDVVELVLL
                     SLFVGGHSIITGVPGLAKTLLVRTIASVLDLSFKRIQFTPDLMPADITGTEIIEEDRA
                     TGRRALQFIRGPVFANIVLADEINRTPPKTQAALLEAMQEGRVTVQGVSYDLPRPFHV
                     LATQNPIEMEGTYPLPEAQLDRFMFQIRIGYLPEEDEVAVVRQTTSPQDYEFTPQMTA
                     DEIVAFQGLVRQVPAPDTVTRYAVRLAAASRPGSAGAPDFVNRWVTWGASLRASQFLV
                     "
BASE COUNT         2555 a         5011 c         5113 g         2380 t
ORIGIN      
        1 caggctttct tctggtccac gttcgggcgt cagctcccct tgagcgccgt gggacaatcg
       61 gcgacgcacg accgcttcgg ctacgaccac cagcacgcgg ttgacgtgcc gctgcacccc
      121 gacagcctgg agggccgcgc cgtcatcagt tatctgcaag cacagggcat caccttcacc
      181 gccttccgcg ccgccatccc cggcgttgcc accggcccgc acatccacat cggccgaccg
      241 tcaaatcgac tctcataaca ggacactgcg gcgcgcacag cgtgaccgta acgtgacagt
      301 tttcatcctc gcccggtgta accggagcga cgaggaaggt gtctatcaat acgtggggca
      361 gtagaagagt tttactgccc ctttgactaa taggtggggc gctcgggcgt cgggggtgaa
      421 cgatggagtg gatgaaatta caactgaacc gcaagcggcg catggccgtg gtgctcgtgg
      481 cgattgtcgt cgccggcgtc gccggtttct ggtactgggg cgacggcgcg agcgccgcgc
      541 cgtacatgac cggccgcgtg gagcgcggga gcttgcgtaa caccgtcacc gcgacgggca
      601 cgcttcaggc cgtcaccacg gtgcaggtcg gcagtcaggc gtcgggcacc atctcggcgc
      661 tcctcgttga tttcaactcg atggtgaaga agggacagat catcgcgcag ctcgacccgg
      721 cagtctcgca ggcgcaggtg cagcaggcgc gcgccaacct cggacaggca caggccggtc
      781 tggagcaggc gcgcgcgggt gtggtgcagg cgcgggcagg tctggccgac gcgcgcgcca
      841 aggcgctctc cgccggctcg accgcgcagg ggcagcgcgc cggagtgtcc ggcgctgagg
      901 ccaacgtcgc cgtgctcaag gcgcagttag acgacgcggc ctccttcatg cgacagcagg
      961 agtctctggt gcagagcggc atcatttcgc agcgtgagct ggagacggcg cgcacgagtt
     1021 acaggacggc cgaggcgcgc tacaaccagt cgcaggcaca actgaatcag gcggtcgtcg
     1081 gcgaaaagtc cgcgtcaagc tcgggacagg cgcaggccac ggcgcaggtg cagaacgcgc
     1141 aggcgcagat cgtgcaggcc gaggcgcagg tgcggaacgc gcaggcgcag gtgcagcagg
     1201 cgcaggccgc gctccaactg tccgagttga acgcgtcgca cacgaccatc cgctcgccga
     1261 ttgacggcgt ggtggtctcg cgcaacgtgg acgtggggca gacggtggcc gcgagtcttt
     1321 cggccccgac gctttttacc atcgccaacg atctgacgca gatgcaggtc atcgccaaca
     1381 tcgatcaggc ggacatcggc ctcgtcgagc aggcgaaggg cgtcaagttc agcgtcgatg
     1441 cgttccccga caaagagttc gagggcacga ttcggcagct cagactcaac cccgtgagcg
     1501 cgcagaacgt cgtcacctac aacgtggtga tggacgtgtc gaacccggag cagaagctca
     1561 agcccggcat gacggcgaac ctctcgatca cgattgacga gcgacaggac gtgctcaagg
     1621 tgccgaacgc cgcgctccgc ttcacgccgc agctcacgga agagcagcgg gccgcggcgg
     1681 ccgagcgacg cgccgcgggc gggcagggtc agggcggcga gcgacgcgcg cgtcgtcagc
     1741 agaatgagaa cacgaacgcc ggcgcaccgg atgctaacgc cacggcgacg aacgccgcgc
     1801 cggccgacgg cgggcgccgg ggcgacttcg cttccgcgac ctcgccggtg acggcaggcc
     1861 agacgcggcg cgtgtgggtg atgggcgcgg acggccagcc tgagccgcga cgcatcaagg
     1921 tcggtctgac ggacggcgcg gcgaccgaag tggtcgaagg cgacttgcgc gaaggcgagg
     1981 tcgtcatcac gggccagaac gtcacgggca cgcagcagca gaatcgtcag cagggcgcgc
     2041 agtccgcgcc gggcttcggc ggcgcgccgc gcaccggcgg cggtggtggc ggcggccggc
     2101 gtaactaaca acggcgcgag ggacaggcga cggcgtctgt cgccgaagcg acgaaaacgc
     2161 atcggcgtcg gacgccgttc gcaggttgtg acgcggggga gttgaagcga tgcagacggc
     2221 atacgaaatt gggcggcaga cgtttgaagc gggcgcggga gtcgccgctt cgcgcgcaga
     2281 gcagcaggcg cggcccgtca tcgagttgga ggggatacac aagacctacg cgatgggcga
     2341 tgtggaagtc cacgcactac gcggcgtgtc gctcacgatc cgcgagggcg agttcgtcgc
     2401 tatcatgggc acctcggggt cgggcaagtc cacgatgatg aacatcgtcg gctgcctcga
     2461 ccgccccacg cgcggcgtct acacgctcga cgctcaggac gtgtcgggca tgtcgaagga
     2521 cgagcgcgcc gacgtgcgta gcagcaagat cggcttcgtc tttcagggct tcaatctgct
     2581 gtcgagaacg tcggcgctgg agaacgtcga gctgccgatg ctctatgcga acgtcgagac
     2641 ggccgagcgc aaacgccgcg ccgtggaggc gcttcattcg gtgggcctcg cggggcgcga
     2701 gcagaaccac ccgaaccagc tctcgggcgg gcagcagcag cgcgtggcga ttgcgcgctc
     2761 gctcgtcaac aacccgacgc tcatcctcgc cgacgagccg accggcgcgc tcgactcgcg
     2821 cacctcggtc gaggtcatgg agatttttca gcgcctcaac cgcgagcgcc gcatcacgct
     2881 cgtcctcgtc acgcatgagc cggacatcgc cgcctacgcg gatcgcgtcg tcgttttcaa
     2941 ggacggacgc atccagaagg actaccgcgt ggacgcgccg cgcgacgccg ccgaggagtt
     3001 aataaatctg ccggaaatag aaccggaaga ggactgagca acggatatta gttatccatc
     3061 gtatagcctg aagagaggaa catgaacgca ctagccatca tccgtatcgc cttccgcgcg
     3121 ctggcccgca acaagatgcg cgccgccttg accatgctcg gcatcatcat cggcgtctcg
     3181 gccgtgatcg cgatggtgag catcgggcag ggcgcgcagg cgtcggtgca ggcgcagatc
     3241 gagggcatgg gcacgaacct gctgtttgtg agcgcgggcg cgcagaacac gggcggcgtg
     3301 cgcggcggcg ccgggcagga cggcggcaac aacaccttga acgccgaaga cctcgaagcc
     3361 atccggcgcg aggtgcccag cgtctcgatg gtcagccccg tggtcaacgc gcgttcgcag
     3421 ctcgtcgccg ccaatctcaa ctggaacgcg tccgtgcagg gcgtgagcca acagtacccg
     3481 gacatccgca agtgggcggt gcaatcgggc gagttcttca ccgacgcgga cgtgcggacg
     3541 gccgcgcgcg tcgtcgtgct cggtcagacc gtggccgaca acctcttccc cggcgccgac
     3601 cccgtggggc agacggtgcg cgtgacgaat ttgccgttcc gggtcgtcgg cgtcatggcc
     3661 cgcaaggggc aagacccgca ggggcgcgat caggacgacc tcgccttcgc cccttacacg
     3721 tccgtccaga agaaacttct cggcaacacg cgcctccaga tcgcctacgt ctcggccgtc
     3781 tcgcaggacg ccacctacac ggcgcaggcg cagattaccg accttctccg ccagcgtcac
     3841 aagctcgccc cgaacgaggg caacgacttt acggtgcgca acatgtcgga cgtagccgag
     3901 gccgccaatg agaccaacag cacgatgacc attttgctcg gttgcatcgc cggcgtgtcg
     3961 ctcctcgtcg gcggcatcgg catcatgaac atcatgctgg tgtcggtgac ggagcgcacc
     4021 cgcgagatcg gcatccgcat ggcgattggc gcgcgctcct cggccgtgcg gacgcagttt
     4081 ttgatcgagt caatcgttct cagtctgacg ggcggagtgc tgggcatcgt gctcggcgtc
     4141 ggcgtttcgg tctccatccc cgcgatgctc ggctggccga cgctcgtctc caccgcggcc
     4201 gtcatcggct cggtggtctt ctcggccgtg gtcggcgtct tcttcggcta ctacccggcg
     4261 cgcaaggccg ccgcgctcga cccgattgag gcgcttcgtt atgagtgacg acaaatcact
     4321 tcggaagatt ttcaactgac agcgggccgc actcggcgag cgcgcgcccg cgtttacgaa
     4381 gaaagacgat gacgaacaca gaggttttaa aaatgtggcc cggcgtcgaa acggcgactg
     4441 ctcccgaggc ggctgcccct gagtcggcgc gcggcagaga ctttgagctg gcccagcggg
     4501 ccggcgcggg tgatgcggag gcgttcgagc agctctacac gctgcaccac cggcgcgtct
     4561 atggcctgtg cctgcggatg atgcagaacc cgacggaggc cgaagacctc gcgcaggacg
     4621 ttttcatcca gctcttcaaa aagatcggaa tgttccgcgg cgagtcggcg tttacgacgt
     4681 ggttgcaccg gatgacggtc aatcaggtct tgatgcactt ccgcaagaag ggcgtccgcg
     4741 tggagcagac gacggagagc ggcgagatga ccgagcaggt ggtcgcgggg acggaggatc
     4801 acgcgcggat gcccgtcgtt gatcgcatcg cgctcgacaa cgccatcgcg aagttgccgc
     4861 ccggctaccg cgccgtcttc gtcctccacg atgtcgaagg ctacgaccac gcggaggtcg
     4921 ggcgaatgtt gggcgtggcg gccggcacct ccaagtcgca actgcacaag gcgcggatga
     4981 aactgcgtgg cctgctcaag cggcctcggc cgctccgctg agtcggcggc ggcgtcttcg
     5041 ccgcgagggt aaataaagac gcagttactt tcgtcgcggc gcgggcgacg gcgtcggacg
     5101 cttgacgccg cgacggcttc ggcgggataa gttaaccaat acaaaaaatg aaggccggct
     5161 attcacccgc caaggggtga gtagccggcc ttcagagttt cgacgtggga ggttcctgcg
     5221 cgagcgtctg aagtgagatg ggagggcgtc aggcgtcctg ctttattttg aagatgctgc
     5281 gccggcgcga caacgccggc gtgcgcgcgc cgagcaggtc gttgtgccgc cccttgagga
     5341 ttgaggatag cgagtggtgg tgcttgaggc cgagcagccg cgacgcgtgc gtcaccgagc
     5401 cgccggcttc cttcagcgct cgctcgatca gcaaactctc gaagcgccgc acctcttctt
     5461 tgaagttgaa tcctttccaa tcggttggta gcccgaacac ttccgcgccg tcctcgccct
     5521 gagccgccgc cgagcctgag ccgacggctt tgaatgcggc caccgcccgg cgggcgcacg
     5581 cgttcagtcg cgccagcgtg ccggggtgtt gcgttttgcc gagtagatcg tcggcgctcg
     5641 cataggcgtc gcacagttcc tcgggactga ggtgctcgcc cacctcctcg atgacgacga
     5701 gcactgcgcg ccccgcgcct tcccggtcgc ctgccccttg cgccacttcg attgcgcgtc
     5761 gcagcgtcgc gagcgcctgc gggaagcggc ccgtgcgtgc cagcgccgtc ccgtgcgtcg
     5821 tcaacgcctc ggccagcagc gacaactcgt cgcccctttc gagcgcgcgc acggcagcgc
     5881 gcgccacgcc ttcggcctcc gcgtcgcggc cctcggccag caggacgcgc gccagcgtct
     5941 cgtccacctg cgccacactg ccgccgtctc cgagcttcgc gaacatcttc cgcgcgcgcg
     6001 ccgcgtgctc gtgcgcctcg ggcgctcggc cggcctctac aaataacatc gccaggttat
     6061 tctcgacgcg cgcgcagtag cgctcgtggc ccgcctgctc gaagtggaag ctggcggccg
     6121 tgaactccat gagcgcgcgg tcgatgtagt cctcgcgatg ttccgccatg ccgatgttct
     6181 tgagcgtggt ggcgaagccg ccgtggaact tgccccggag cacgtggttg ctaacatgct
     6241 cgaagaaggg cgcggcgtcg gtgtagacgc gcagggagtc gccgagtctg ttcgccacgg
     6301 tctcgacaat cgcgctccga agcagcgcga cggccttcac ctcgctgtcg tcaggcagcc
     6361 gactcaaagc ctcacgcagc cacgcgcgcg ccccgtcgta ctcgccctgc cgccagtagc
     6421 acaccgccag atcggactgc gcctcggccg tcttctccac gtcccccagc gcatcaaaga
     6481 tcgcggcggc ttcgctaatc aaatccttcg ccacctcctg cgctccctcg acctgcttga
     6541 cgctcccgag accactcgtg agcacgccgg cctgcaatag cacttcggcc gccgccctcc
     6601 ggtcgagacc ttcgacggcg ggcacttcgc cgacgcgtga ccacagatcg cccatcgccg
     6661 cgcgggctcc ttcgtagtcg cctgactcct caagcgccct ggagagttgg acgcgcagcc
     6721 tcgcgcgctc gccgtgggtg agcgcggcgt cggccattcg ttcaaccagc tgttcggcta
     6781 acttcatgtg agggttacct ttctttgtgc ttgatgattc ttcgtcatcg tgctgaaatg
     6841 cgtccgcgca tcctgggtgc ctcgggcgac agggagcgcc gacggaactt gttcgggtgc
     6901 tcggtggaga tgatccatcg ttcctagtga cccatcatct taatttccaa tcacaggaga
     6961 aaccaatgaa aacgttacgc caactcagcg cctcggcctt gctgatgctc gtgtttgccc
     7021 tgcccgcctt cgccggtgac atgcacttcc ccgtggcgcc gccccagccg ccccccgcgg
     7081 ctcccgcgcc gctgacccag ccgaactcca cgtcgggaga tgcggccctg ggtgtcgaga
     7141 cggccgatgt cgatccgctg acggagatca cgctgctcat gctgcaaggc gtgctggcct
     7201 tgttttaaga aacgcgccgg cacgaatcgc cccggtctac cgtgagcctg ctgcggccac
     7261 ggccgcccaa gtgtaggtgt acctgaaggg aacgccggga ggcaagaatg cctggcgagg
     7321 aagcacccac ggggcgaaag attaactccg cattgcagag aaagcaataa actttttcgc
     7381 cggcatgcgc ctaccggagg ttgaccggag gcgcgcgccg gggcggagcg cctgaaggac
     7441 acgacctgaa cgaggggccg cgggccgggc cgtggggccg cctccagcct ccccgcgccc
     7501 ggcggcgcgc gcgcgcgcgg ccctgccgcg atgtgccgga aacgttcagg cggccgagca
     7561 tattcagtgt gccggccgcc cgtgcgggcc aagttttaca acccttttac gtcatcgcaa
     7621 acattttttc cgggtcgtga ttgactatac gccggaggct taagcatcag gaaaactcga
     7681 accacgggcg aaatgggcag gggttgcact tctactttcc cgtccccgga cggactcacg
     7741 aatcacccac cttccgacgc gaggcggcgg ctcacgcgtt aacccttgcc gtttctgccc
     7801 gcggggcgtt aattttcata tccggcggcg tcaggtgaac tactcacctc tcgtcttatc
     7861 tttttagggc ggcgcaccgt cgcgcgcgcg cgggacatca aaacgtatcc tcgctgggag
     7921 accgtccgac gctgcccaag cgggccgcgt ccgtcaaagt gtcggcgcat ggatcgactc
     7981 gtacagtttt tgtcggggca cgaaccggcc gttttcagca atggccagtt cggctttgac
     8041 gtgcgcctgc accccgccct gctcgccgtc ctcgcactcc tcttcgccgc cctcgtctat
     8101 ttcgtctacg ttcgggcgcg cgcccgccca cccggccgac gcctcgcggg tctggccgcg
     8161 ctgcgggccg cgctcttcgt cctgctcgtc ttcctgctga tgaagccggt cgtcgtcgtg
     8221 ccgtcggtcg tgccgcgcag cagctccgtc gccctcctct tcgacgactc gcgcagcatg
     8281 caacagccgg acggccctga gcgccggacg cgcctcgacg caatcagaga cacactgctc
     8341 tccgccgagg gcacgttcct gcctcgcctg cgcgagaagt tcaggacaga cctctacgcg
     8401 ttctcgggcg agctgacggg gcttcgaggc ggcgacgaac tgctgggcga ggggacggcg
     8461 agcgatccgg gcggcgccat cgcggaggcc gcgcggcgcg cgaccgtcgt gccgctctcg
     8521 gccgtcgtgc tcgtctccga cggcgcggcg aacgtccctt cagacctcgc ggccgagctg
     8581 cgaaatctgc gcgcgcgcgg cctcccggtc tacaccatcg gggtggggag cgccgcccgg
     8641 cagaccgacg ccgagctgtt gcgcgtgagc gttccccgcc gcgtgctcgt cgggtcgagc
     8701 gcgcaggtgg aagcgttcgt gcgtctcgcg ggctacggcg cgacgaaggt gctcatcgcg
     8761 gtcagcgagg acggccgcgc catcaggacg gaggagttct ccctgcgcgg ggccgagacc
     8821 gaggccgtcc gcatggagat cgtcccgacg actccgggca cgcaccgcta caccttcgag
     8881 atcacgccgc tcgacggcga gttgagcgtg gagaacaacc gccgggaggc gctcatcgag
     8941 gtcgtcgagg ggccgcggcg cgtgctctac gtcgagggcg agccgcgctg ggagcacggg
     9001 aagatgcgcg cggccgtcgg gcgcaacgag aagcaggtgg aactcgtctc cgtgctgcgc
     9061 tcgggcgaga acaagctcta ccggcagggc gtggcgggcg agcaggagtt agccgcgggc
     9121 ttccccgcca ccgaggagga gctgttcgcc taccacggcc tcgtcctcgg cagcgttgag
     9181 gcgagcttct tcacggccga gcagttgcgc gccgtcgagg ctttcgtcgc gcggcgcggc
     9241 gcggggctgc tcgcgctggg cgggcgtctc gccttcgacg gcgggaagta cgcgggcacg
     9301 cccgtcgccg accttctgcc cctcgcgctc gacgggcgcg ccgggcagcc ggaagacctg
     9361 agcaaccccg tctacaaacc tctgctcacc gcgcgcgggg cgacgcaccc cgtcacgcgc
     9421 ctcggcgaag accgcgcgct gagtcagaaa atctggaacg aactgccgcc ggtctccgtg
     9481 ccggaaacgt tcggcggcgt gaagcccggc gcaaccgtct tggtcgaagc gcgccgggag
     9541 ggcggcggcg cgaccgtccc gctcctcgcg catcagcgtt acgggcgcgg gcagacgatg
     9601 gcgcttgcga cctccgacac ctggcgctgg cagatgcgga tggactcgaa gagcggcgcg
     9661 cacgaaaact tctggcgaca gatgctgcgc tacctcgtca gcggctcgcc cgcgcaggtg
     9721 gaggtggcga ccgagcagga cgtttacgcc gtgggcgacc cggttcgcat cgtcgccgac
     9781 gtgagggaca aaaaatacaa cgcggtgacg ggcgcacgcg tcgcggcgcg cgtcgtcaag
     9841 ccgtcgggcg cgagcgtgga tgttccgctc aggtacacgg cgcgcgacga cgcgaacgtc
     9901 tacgtcggcg agttccgccc cgacgagttg ggacagcacc gcgtcgagct gagcgcggcc
     9961 ggcgagggcg tgggtgaggg ggcggcgcag tcggaattcc tcgtcgccga gttgaaccgg
    10021 gagttctacg acgcggcgct gaacgaagac ctgctccggc gcatcgccgc cgagacgggc
    10081 ggcaagtact acacgctcga agaggccggc gccctcatcg gcgatctgac ttacaggcgg
    10141 acggacaact cgacgctggt gacgaaagac ctgtgggaca tgccggtgaa tttcgtcctg
    10201 ctcatcggga tggtgagcgc cgagtggttc ctgcgaaagc gcgaggggtt ggcatgacgc
    10261 gtcgtctcat caaagctctc atctgcggcc tgatggttgc cggcgcggcg tgcggcgtga
    10321 tgcctcgcgc acagacgccg gcgcagaccg taccggccgc gcgtccggcc gccgacccga
    10381 acaagttcgc cgtcatcatc agcggcgcgt cgggcgagga ggtttacgcg aaacagttcg
    10441 cgcgatggac gacggagctg cgcggcgtcc tcgtcgggcg tctgggattc gccgaagacc
    10501 gcctgaagat tcttgcggaa agtgctggcg aaggcacgcc ggccgcgcgc gccacggccg
    10561 aggaggtgcg gcgcgcgttc gagtcgctgc ggggcgcggc ggggccggag agcaccttgt
    10621 tcgttttctt catcggccac gggacgttcg acggcaagca ggcgaagttc aatctcgtcg
    10681 gcccggacat cacggccggc gagtacgcga gtctgctcgg cgcggtgccc tgccggcgcg
    10741 tcgtcgtctt caacttgtcg agcgcgagcg gcgagttcgc gaagacgctc gcgggccgcg
    10801 ggcgcgtcgt cgtcacggcg acgcgcagcg ggcaggagca gaacgcgccg cgcttcgccg
    10861 agcacttcat cgccgcgctc acggggcgcg agtcggacgc cgaccagaac gggcgcgtct
    10921 ccgtgctcga agccttcgac tacgccgcgc gtctcaccgc tgagcactac aagcggtcgg
    10981 gccgcctcgc caccgagcac gcgctcctcg aagacagcgg cgacggcgtc ggccacgaaa
    11041 aggccgaggg cggcgacggg gacattgcgc gtgcgaccta cttcgactcg ctcacggtcg
    11101 agcaggccgc cgccaacgcc gaggtgggga gacttttgcg cgagcgcacg cgttacgagg
    11161 aggagatcgg gcagctcaag acgcgcaagc gcgagatggc cgaaggacag tacgagtcgg
    11221 agctggagcg actattcgtc gggctggcga agaccaaccg gagcatcaag aggggcgggc
    11281 agtgaagcag cacgaacaga cacaaaatat acaacgccgg aggtcgcggc aaagcattcg
    11341 tccgtcggtc gtgtgcgccg tcctgctcgc cgtttgcgcg ttgctctact cacaggctcc
    11401 cgccggttcg gcgcagtccg cggacgaggc tttgccgggg ctggccgagt tgaagaaagg
    11461 ggactatgag cgcgccgtga aggtactagg cgcgcggctg gcgacgaacc catcagacgc
    11521 cgcggcgcgc aaacatctgc tgcgcgctta catggagacg gggcactacg cggaggcgga
    11581 aacggcagcg cgggggttcc ttgagaagtg gccggaggcg ggcggcgtgc gtcacgagct
    11641 ggccgaaagt ctggccgcga cgggcaggta cgccgaggcg gtcgtcgagt tcgagcgcgc
    11701 ggcgaaggat ttgtcgaaga ccggagtcct gccggccgag cggttggaga gcgacctgcg
    11761 ccgcgccgaa gtgctccagc tcacggggca ggaggagcgc gcgcgagaga tctttcagtc
    11821 gcttgtgact tttcacgagg agaacgacac ggaggaagat aacgacgcga agtcggcgcc
    11881 cgtgctgacg cttgtcgccc gcgcgctcgc gcaccttgag aagtacaggg aggcgactga
    11941 gctttacatg gcggctgccg aggccgaccc gtcgcacacg gaggcgcacc tcggcggcgg
    12001 cgagttgttc accgagaagt acaactacgc ggacgcctcg gagttcttcg ccgacgcctt
    12061 gcgcgtcaac ccgcatagcg cgcgcgcgca cttagggata gccgccaaca agcgtctcga
    12121 aggcggcgcg gaggtgtcgg ccgcgctgga gcgcgcgctg aagatcaacc ccaacctcgt
    12181 cgcggcgcgc gcgctcaagg ccgcgctcga tctcgaggct ggcgacttcg acgcggcggc
    12241 ggccgagatc gacaaggccc tgaaggtcaa cccgcgctcg tcggaggcgc acgccctgcg
    12301 cgccgcgctt gcttacctgc aagaccgcga cccgttgccg gccgcggcgg ccgctctcgc
    12361 cacgaatccg cgcgacggcc gggtctacga caccctctcg cactacgcca ccatcacgcg
    12421 ccgcaccgcg caggccgccg acttctcgcg ccgcgccgtc gaacttcagc cgcgtctctg
    12481 gcgcgcgcac ctcagtctcg gcatggcgct cttgcgcttg ggccggatgg acgaggggcg
    12541 cgcggcggtc gagaaatctt tcgagggcga cccgttcaac gtctgggcca agaacacgct
    12601 cgatctgctc gactcgatgc gcgacttccg cgaaacgcgc cgcggaaatt tcatcatcaa
    12661 ggccgacgcc aaagaaacgg aagtgctcgc cccctacgcc gccgatctgc ttgaagaggc
    12721 ggcccgtgag ctgacggcga agtatcgctt cacgccgcag gggccggtga tggtcgagct
    12781 gttcaagaac cacgaagact tcgccgtgcg cgctctgggc ctgccgggcc tcggcgcgct
    12841 cggcgtctgc ttcggccccg tcatcgcgca ggactcgccc tcggcgcggc ccgaggggca
    12901 gttcaactgg ggcagcacgc tctggcacga gtacacgcac gtcatcactt tgcagatgac
    12961 cgactaccgc atcccgcgct ggttctccga agggctgtcg gtctacgagg agcggcgcgc
    13021 gcgccccggc tggggcgacg agtggaaccc ctccgtgctg cgtgcgttct ccgaaggccg
    13081 ctggttcaag atcgccgaac tcgactcggg cttcatgcga ccgcgccgcg ccgacgacgt
    13141 ttcgctcgcc tactttcagg cgtcacaggt cgtcgagtac gtcgtcgagc gccacggctt
    13201 cgacgccgtc ctccgcatgc tcgcgctcta tcgcgacaag gcccgcacgc ccgatgttct
    13261 gcggcaggcg ttgaagctca cagaggcgga gttcgaccgc gccttcgttg aatatgtcgg
    13321 cggcaaggcg cggccgctga tagaggcgct cggggccgaa ggcggcgcga cggctgccgg
    13381 gatgccgaag gacgcggtgc tcaagtcgct cggcgagcgg gagaactttg cgctccacct
    13441 gcgcgccggg tcgttgtacc gcgccgaggg cgacgccgcg aacgcggccc gtcacctcaa
    13501 gcgcgccgtc gagttgttcc cctattacac gggcgagggg aacgcctacg aattgatggc
    13561 cgacatcttt gagaagcagg gcgacacggc ggaggcggcg caggctttgg agctgctcgt
    13621 caggctcgac gaaaataatc tgcccgcgct caaacgactc gccgatctgc gactcaagca
    13681 gaatgatgcg gcgcgcgccc tcgaagccct gcggctcggc ttctacgtca gtcccttcga
    13741 ccacgcggcg cacgcgcgcg cgggcgaact gctcatggcc cggagcgaag cggggagcgc
    13801 gctcggggag tttcaggcgg cgctcgcctt gaagcctccg aacgaggccg aagcgaacta
    13861 caatgtcgcc cgcgcctacc acgcgctcgg caaatcagcc gaggccaagc gcgcggtctt
    13921 gcgcgcgctc gaagccgcgc cgagctatga gaaagcgcag gagttattgt tgacgatcac
    13981 ggggcagtga gcggcgcgat tgtaggggca cggacaagtc cgctgcccgc aagttgcgga
    14041 aagaattaac atggatggac aggatacgcg ggatgaagaa agattgattt aagatttctg
    14101 atttgagatt tcaactcttc ttatcctgcg tatcctgtcc atccatgtta attgttcttt
    14161 cggaaagacg ggcagcggac aagtccgccg cccctacaat gacacggggt ggttgagcag
    14221 aaggagagac gaatggctgt tgagacgacc gtgatgcgcg acgaggaact gctggacagg
    14281 ctggcgacgg cgcgcgaaaa actcctgcgc gagattcgca aggccgtcat cgggcaggac
    14341 gacgtggtcg agttagtcct gctgtcgctc ttcgtcggcg gccactcgat catcacgggc
    14401 gttccgggtc tcgccaagac cctgctcgtg cgcaccatcg cctccgtgct cgatctctcc
    14461 ttcaagcgca tccagttcac gcccgacctg atgcccgccg acatcaccgg caccgagatc
    14521 atcgaggagg atcgcgccac gggacggcgc gcgctccagt tcattcgcgg ccccgtcttc
    14581 gccaacatcg tcctggccga cgagatcaac cgcacgccgc ccaagacgca ggccgcgctt
    14641 ttggaagcga tgcaggaggg ccgcgtcacc gtgcagggcg taagctacga cctgccgcgc
    14701 cccttccatg tgctcgccac acagaacccc atcgagatgg agggcaccta cccgctgccc
    14761 gaggcgcagc tcgaccgctt catgttccag atcaggatcg gctacctgcc cgaagaggac
    14821 gaggtcgcgg tggtcaggca gacgacctcg ccgcaggact acgagttcac gccgcagatg
    14881 acggccgacg agatcgtcgc ctttcagggt ctcgtccggc aggtgcccgc gcccgacacc
    14941 gtgacgcgct acgccgtccg cctcgccgcc gccagccgcc ccggctccgc gggcgcgccc
    15001 gacttcgtca accgctgggt gacgtggggc gcgtcgcttc gcgcctcgca gttcctcgt
//
LOCUS       DASVES010000327        12339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_210097, whole genome shotgun sequence.
ACCESSION   DASVES010000327 DASVES010000000
VERSION     DASVES010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210097"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VF240_13490"
     CDS             <1..690
                     /locus_tag="VF240_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7176270.1"
                     /translation="LVGQVPMAVLAALMIMAGIGAINFREARSIWNTGGAARWSISVT
                     FVATLVLSIPLAVAAGVLLTVVLYLKSSASDVRVLELVRLDDGRIAASKPPARLPGNK
                     VTVLDVDGSLFFAGARTLSEALPTPAGATRPVVVLRLRGYTSVGATLVEVLDDYADAL
                     AEVGGRLYLSGVGEEVGRQLRRAGKLDLDRAVHLVPADDIFGASTGQAVAHASEWLGS
                     TRHDSPLTENE"
     gene            900..1436
                     /locus_tag="VF240_13495"
     CDS             900..1436
                     /locus_tag="VF240_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7176271.1"
                     /translation="MLTVFRIAVAVALSSLLVYSSPQTRARQGAGPVKFSFRSLDGGN
                     VTDADLRGKVAVLIFSDRRLPISKKRAESVRFLVYEFAPRGVVFYLISTDSERPESKN
                     YASDEAVRGYGRKSRLRLPVLRDPEGKTLREYGRDQIPLVVVLDRQGRVDGKPLEGFS
                     LKGSLERTLRSRLRRLTR"
     gene            1992..2942
                     /locus_tag="VF240_13500"
     CDS             1992..2942
                     /locus_tag="VF240_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7176272.1"
                     /translation="MNERMKVLVAYDGSAYADAALEDLRRAGLPREAEALIVSVSDGL
                     VDPSSSIADIAGSALTSRRVTSAIELVREHAAQALEEAKEFAAKAGDRVRSYFPDWEV
                     RAEGIAGTPSQELAQRADEWKPDLIVVGSQGRSALGRFFLGSVSKKLATESRSSVRVA
                     RRTVEKGQGAPPRIMIGVDGSHGAEGAVRSVGRREWPDGTEVKIVAVDDGVSPARIAH
                     ILPTAAGIIRSNNEEAAGKARQMAEWAENELRAVGLQVSVAIVKGEPQHILIEEAQKW
                     DADSIFVGSRGLDQPNEKSGLGGVSTGLVMNAACTVEVVR"
     gene            3111..3680
                     /locus_tag="VF240_13505"
     CDS             3111..3680
                     /locus_tag="VF240_13505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7176273.1"
                     /translation="MSKPVRILGIAGSLRRESYNRAALRAATELAPEGATIETFELDG
                     IPGFNQDEEQNPPAQVTELKRRIREADAILIVTPEYNYSIPGVLKNAIDWASRPYGDS
                     AWNGKPAAIMGASVGSIATARAQYHLRQMMVFLNMFPINQPEVMIGNASERFDAQGKL
                     TDDATKDFIRQLLQNLVEWTRRIGQPQGT"
     gene            3717..5132
                     /locus_tag="VF240_13510"
     CDS             3717..5132
                     /locus_tag="VF240_13510"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEX7176274.1"
                     /translation="MPEIESYDVLVIGSGEAGKYLAWTMARAGHRTAVVERRYIGGSC
                     PNIACLPSKNVIHSAKVASLAARAAEFGVEVGPVATNMEAVQLRKRKMVEDLIKVHSD
                     RYEASGAELIMGEARFVSPRTVRISLNDHGTRVIAGERVFLNVGTRAALPPVPGLAEA
                     KPMTHVEALDLDRLPEHLIVLGGGYVGLELAQAVRRFGSRVTVIERGPRLASREDTDV
                     SAAFLELFHDEGIEVLLGAEVRRVEGRSGGEIRVSVEEADGGRVIEGTDFLVAAGRTP
                     NTQGMGLDLAGIELDRRGYVRVNERLETTAPGTWAMGDCAGSPQFTHVAYDDFRVVRD
                     NLNGGNRTTENRLVPFCMFTDPELARVGLNESEAKNSGIDYRLVKLPMAAVLRTRTIS
                     EPRGFIKMLLDVASDRILGITVLGAEASELMAVVQTAMLGELPYTVLRDAIFTHPTAA
                     EGLNVLLADVAPKAMHEFATS"
     gene            5209..6255
                     /locus_tag="VF240_13515"
     CDS             5209..6255
                     /locus_tag="VF240_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX7176275.1"
                     /translation="MRAVRFAGVGKSAQIADVAKPSPAPGQVLIKIGGAGVCHSDLHV
                     MEEDLGFTPPFTLGHENAGWVAELGEGVTGFKEGDAVAVYGPWGCGRCHACQLSMENY
                     CENWADLGGFGGGLGFDGGMAEYFLVPAARLLVPLEGLSPAKAAPLSDAALTPYHAIK
                     RALPRLNADDTVVVMGVGGLGHMAIQLLRVLAPVRIIAADVDEKKLEQAKALGADEVV
                     NNRDADAAAERILKLTGTRGAGLVLDCVGVQPTIDLGAKLLGRNCVWTIVGLGGGHHD
                     FHHGSTPYGCSMSIPYWGSRVELMEVIAMARDGRIHAETTEFPLEQAVEVYDKLKAGQ
                     ITGRAVLIPDGASR"
     gene            6283..7539
                     /locus_tag="VF240_13520"
     CDS             6283..7539
                     /locus_tag="VF240_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7176276.1"
                     /translation="MSSINEVLAGLQAVSAWQERVYKDFHAHPELSFQETKTAALAAS
                     KLRELDYEVHEGIGRTGVVGILRNGEGQTVLARADMDALPVRENTGLPYASTLTAADM
                     DGETVSVAHACGHDVHVTCLLGAAELLAKARRSWGGTFVALFQPAEEVAGGAKAMVED
                     GLPDRIPKPDVAFAQHVLAYPAGTIGTQAGPVLSAGDSIRLTLHGKGSHGSMPHNSVD
                     AVVLAAMVVVRLQTIVSRETKPGEFAVLTVGSSVAGSKSNIIPDRAQLLVNLRTYDTD
                     VRRRMLDSIERMVRAECEASGSPRPPEFEYYDQYPLTDNDSEVTEKITRAFKDYFGAE
                     TLFDLGRQTASEDFSLIPDAFGTPYTYWGIGGTDPEQYERAHKAGRVEQDIPVNHSEF
                     FAPVIEPTLSAGTRALVVAALTYLSK"
     gene            7558..8298
                     /locus_tag="VF240_13525"
     CDS             7558..8298
                     /locus_tag="VF240_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX7176277.1"
                     /translation="MIQLPGSEEALRALAEFKAYAAGTLLVLDLVGTFVFALSGAVAG
                     VKERLDLFGVLVLSFAAASAGGIMRDLLIGAVPPAAISDWRYLGVSLLAGLVIFFWFP
                     RSERLQKLRNLVLVFDGAGLAVFAVAGTQKALGYGLNPLMSALLGMLTGIGGGMLRDA
                     LLAQVPTVLRSELYAVAALAGASVVVVGHVLNFPPTAMAIAGAVLCFGIRLVAIRRGW
                     RLPVADLPQRFEAGAGGADDQQDKVGKF"
     gene            8372..9319
                     /locus_tag="VF240_13530"
     CDS             8372..9319
                     /locus_tag="VF240_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7176278.1"
                     /translation="MNSDAAQKQPDTAEGAVEMVIETRARDLVEGFKVRRALPSRGRR
                     MVGPFIFLDQMGPEVLRAGKGLDVAPHPHIGLATVTYLFAGELLHRDSLGTVQPIRPG
                     EVNWMTAGRGIAHSERTPQEMRLSSSELFGIQSWVAFPTHHEETEPAFAHHGASELPV
                     IEGEGKLVRLICGSLYGARSPVQTFSEMFYADITLEGGARLPVPAEHEERAAYVVEGS
                     IGLIPGGGTFHAGQLLIFKPGEEINLGAPESSPARVMLLGGEPLDGKRHIWWNFVSSS
                     RERIEQAKEDWKAGRFSPVPEETEFIPLPETGPAVVRYP"
     gene            9520..10410
                     /locus_tag="VF240_13535"
     CDS             9520..10410
                     /locus_tag="VF240_13535"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7176279.1"
                     /translation="MQPITKEKSVKKLRRVQRSTERHWVGDGFPVRTLFAYSDRGAEL
                     SPFLLLDYASPMEFPPTDKRLGVGEHPHRGFETVTIVYEGEVEHRDSSGGGGRIGPGD
                     VQWMTAAGGIVHEEFHGRDFARRGGVFEMVQLWVNLPAENKMSPPGYQAVLNSEIPAV
                     SLPDGQGTVRVIAGEFAGAKGPAKTFTPMHVWDLRLASGRQAELAVPDGYTTALVVLK
                     GALRVSGSEAVEASEVGLFDREGQSIHLECVEDTTALLLCGEPIDEPIVGQGPFVMNT
                     SQEIRQAMADYWSGKMGHLS"
     gene            10450..11016
                     /locus_tag="VF240_13540"
     CDS             10450..11016
                     /locus_tag="VF240_13540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX7176280.1"
                     /translation="MTRILGISGSLRRGSYNSALLRAAVPLLDAGVELEIASLHGIPL
                     YDGDLEAAEGLPQAVRDLKARVVACDGLLLATPEYNNGIPGVFKNAIDWLSRPPSDIP
                     QVFGSRPVAVVGASPGGFGTILAQNAWLPVLHALGTRPWFGGRLLVSRASHVFNDTGE
                     MVDEAMRARLRDFLRGFAQFIQASTAPQ"
     gene            11104..11595
                     /locus_tag="VF240_13545"
     CDS             11104..11595
                     /locus_tag="VF240_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX7176281.1"
                     /translation="MNSQATSQSPPISEMAGIKFIAVESFREQIDEFAQVLEARAYEI
                     YERRGREDGHALEDWSRAEAELRCMVPVGILEGPEQLEVNIRLVGCLCHEIEACLEPR
                     RLLLRAQKEEEESQKPGENDAEQRLNGIFLALNLPDQVDPAKVTAVFKDENLCLTLPR
                     AFA"
     gene            11897..>12339
                     /locus_tag="VF240_13550"
     CDS             11897..>12339
                     /locus_tag="VF240_13550"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7176282.1"
                     /translation="MVDEFGAPVAGAQVSLIVKESVVAHTATGEDGRFFLNTVTVAEG
                     TLVFSAHGFAEIKREWSTANREAKGLRIVLAPAPLAEQVVVTATRTEVRLGDTAASVT
                     LVSSEELGATAALRLDDALRQIPGFQLFRRSGSRTANPTSQGVSLR"
BASE COUNT         2376 a         3593 c         4087 g         2283 t
ORIGIN      
        1 cttgtaggtc aggtgccgat ggcggtgctg gccgcgctga tgattatggc gggcatcggt
       61 gccatcaact tccgcgaggc acgttcgatc tggaataccg gcggggcggc gcgctggtca
      121 atctcggtga ccttcgtggc gacgctggtg ctctcgatcc cgctcgcggt cgccgccggc
      181 gtcctgctga ccgtcgtcct ctatctcaag tcgtcggcga gcgacgtgag ggtgctggaa
      241 ctcgtccgat tggacgacgg gcgcatcgcc gcgagcaagc cgcccgcgcg actgccgggc
      301 aataaagtga cggtgcttga cgttgatggg agtctgttct tcgccggggc gcgcacgctc
      361 tcggaggcgc tacctacacc agcgggcgcg acgcgccccg tcgtcgtctt gcgcctgcgg
      421 ggatacacga gcgtgggcgc gacgctcgtc gaggtgctcg acgactacgc cgacgcgctc
      481 gccgaagtgg gcgggcggct ttacttgagc ggcgtgggtg aggaggtggg caggcagttg
      541 cggcgcgcgg gcaagctcga cctcgaccgg gccgttcatc tcgtccccgc cgacgacatc
      601 tttggtgcct cgaccgggca ggcggtggcg cacgccagcg aatggctcgg cagcacgcgc
      661 catgattcac cgctgacgga gaacgagtaa gccactgccg cggtcgtttt gagcagccgg
      721 gcgaagttgg tttgttcagc ctcaagaaga gagatcgata atgttcgtca tactcgcgca
      781 caactggtgg atgctatgct tgcgcggcat cctcccgctc gtcttcggct tgctctgctt
      841 ggtctatccc ggtggcgtca cgttaaccgt catgaaatca tgtgaagcga gggacaaaaa
      901 tgctgacagt ttttcgtatc gcggtcgccg tcgctctgtc gtcactttta gtttactcat
      961 cgccgcagac gcgcgcccgg cagggggccg ggccggtcaa attttccttc aggtctctgg
     1021 atggcggcaa cgttacggac gccgacctgc gcggtaaagt ggcggtgttg attttcagcg
     1081 acagacgtct gccgatctcg aagaagcggg ccgagtcggt gcgcttcctg gtctatgagt
     1141 tcgccccgcg gggagtggtc ttctacctga tcagtacgga ctccgagagg ccggagtcga
     1201 agaactacgc ctccgacgaa gcggtgcggg ggtacggccg aaaaagcagg ctgcgattgc
     1261 cggtactccg cgacccggaa gggaagacct tgcgggaata cggcagggat caaattccgt
     1321 tggtcgtcgt gctcgacagg cagggccgtg tagacgggaa gcccctcgaa gggtttagcc
     1381 ttaaaggctc tctcgaacgc accttgcggt cccggctgag acgcctgacg cggtagtctt
     1441 ctttagccgt agtgcgacgg ccgggcttta tcgcctcgcc ggcgcgctcc gcaagtttat
     1501 caaacacgta ttacgttaac gtgaaggcct tcacggcgag gtcgaaacca acgcggtctc
     1561 agccgcgttc gtccaatccc ggatggcgac atcttcatca cccacctctc cggcatcaac
     1621 cacaaatcct ctccggtcta aacatcaccc gccggggcgc gcccgccgcc tcataaagtc
     1681 aggattagaa aataaaagtc aagaattgga aagaaagtga gcaggggttg ggctaaactg
     1741 ccaaatccct tttcagaaaa gaaagtgcgc attggtgggc taaccattga gttgaaattc
     1801 aaacgcgact taactgcgac caagccggat gaggatgccg aaagaagcca ggcgacgccc
     1861 gactcagaat atctgaattg gtgtgaccgt aatgcgccgc ctcatgcgcc gcttcgtcga
     1921 gcacggaaac tcacaatgac ccttctgaaa ttacttattg attcggtgat gtgggaagtg
     1981 ggggaagcag gatgaacgaa cgaatgaaag tgctggtcgc atacgacggt tccgcttacg
     2041 ccgatgccgc gcttgaagat ttgcgtcgtg ccggactgcc gcgcgaggcg gaagccctga
     2101 tcgtttcggt gagtgatggc ctggtggacc cctcctcgtc aattgcagat atcgccggga
     2161 gcgccctgac ttcgcgcagg gtaacgtcgg ccatcgagct ggttcgggag cacgccgcgc
     2221 aggcgctcga agaggcgaag gagttcgcgg ctaaagcggg tgatcgtgtc cggtcatatt
     2281 tcccggattg ggaagtacgt gccgaaggca tcgcgggcac gccgtctcaa gaattggcac
     2341 agagggcgga cgagtggaag cccgacttaa ttgtagttgg ttcacagggg cgctccgccc
     2401 tggggcgatt cttcctcggc agcgtctcga aaaagctcgc caccgagtcg cgctcgtctg
     2461 tgcgtgtcgc gcgtcgcacg gtcgaaaagg ggcaaggcgc cccgccgcga ataatgatcg
     2521 gggtggacgg ttcacacggc gcggagggtg cagtccgctc ggtgggcagg cgtgagtggc
     2581 ctgacggaac cgaggtgaag atagtcgccg tcgatgacgg cgtttcgccg gcaaggatcg
     2641 cacacatcct tccgacggcg gcggggatca tcagaagcaa caacgaagaa gcagcgggga
     2701 aagcacggca gatggcggag tgggcggaga atgagctgcg cgccgtcggc ttacaggtgt
     2761 cggtcgcaat cgtgaaaggc gaaccccaac acatcctgat cgaagaggca cagaagtggg
     2821 atgccgattc catcttcgtc ggctcacgtg gattggacca gcctaacgag aaatcaggct
     2881 tgggcggcgt ttcaacgggg ctggtaatga atgcggcctg tacggtcgag gtcgtgcgct
     2941 agagcaaccg gcgttgatgc ggcttgattt cgtgcgacta agttagggag tcttatggaa
     3001 caggcgccga gcgattctac gggcgatggg cggcggaggc gctagcgcct taatcctgat
     3061 tgccacccga caagtaatct aaatccgact gacagcaagg agaccatgaa atgagtaaac
     3121 cagttcgcat tcttggcatc gccggcagcc ttcgtcgtga atcgtacaac cgcgccgccc
     3181 tgcgcgccgc gacagaactc gcgccggaag gcgcgaccat cgaaactttt gagcttgacg
     3241 gcatccccgg attcaatcag gacgaggaac agaacccgcc cgcccaagtc actgagttga
     3301 agcggcgcat tcgggaggcg gacgccatcc tcatcgtgac gccggaatac aactactcca
     3361 ttccgggcgt gttgaagaac gcgattgact gggcgtcgag accctacggc gacagcgcgt
     3421 ggaacggcaa accggcggcg atcatgggcg cttcggtcgg ctcaatcgcc acggcgcgcg
     3481 cccagtatca cctccgccag atgatggtct tcctcaacat gttccccatc aaccagccgg
     3541 aggtgatgat cggcaacgcc tcggagcggt tcgacgcgca ggggaagctg acggatgacg
     3601 cgacgaaaga cttcatccgc cagctactac agaatctcgt ggagtggacc cggcgaatcg
     3661 ggcaacccca agggacgtaa cttcggttcg gcaaagtcaa tcagcgaggt agaaacatgc
     3721 ctgagatcga aagctacgat gtccttgtca tcggaagcgg agaagcgggc aagtacctgg
     3781 cctggacaat ggccagggcg ggacaccgca ccgccgtcgt cgagcggagg tacatcggcg
     3841 gctcctgccc gaacatcgcc tgcctgccga gcaagaacgt gattcatagc gcaaaagtag
     3901 cgtcgttggc tgcacgtgcg gcggagttcg gggtcgaggt aggtccggtc gcgacgaaca
     3961 tggaagccgt ccagctccgc aagcggaaga tggtcgagga cctgatcaag gtccattccg
     4021 accggtacga agccagcggc gcggaattga ttatgggaga ggcccgcttc gtttctccga
     4081 gaaccgtccg catcagtctc aatgaccacg gcacaagagt catcgccggc gagcgtgtgt
     4141 tcctgaacgt gggcacgcgc gcggccctac ctcctgtgcc cggcctggcc gaagccaaac
     4201 ccatgacaca tgtcgaggct ctcgatctcg accgtctgcc ggagcatctt atcgtgttgg
     4261 gcggcggcta tgtcgggctt gaactggcgc aggccgtccg ccggttcggc tcgcgggtga
     4321 cggtgatcga gagaggccct aggcttgcca gccgcgagga cactgacgtc agcgcggcgt
     4381 tcctggagtt atttcatgac gaggggatcg aggtcctgct gggggcggaa gtgcgccgcg
     4441 tcgaggggcg ttccggcggt gagattcggg tcagcgtgga agaggcggac ggcgggagag
     4501 taattgaggg gacggatttc ctggtcgcgg ccggccggac tccgaacacg cagggcatgg
     4561 gactcgactt ggccggaatt gagctggacc ggcgcggcta cgtcagggtc aacgaacgtc
     4621 ttgagaccac cgcgcccggc acctgggcga tgggcgactg cgccggcagc ccgcagttta
     4681 cgcatgtcgc ctacgacgac ttccgcgtcg tgcgtgacaa cctgaatggg ggcaatcgga
     4741 cgaccgagaa ccggctggtt cccttctgca tgttcaccga tccggaactg gcaagggtcg
     4801 ggttgaacga atcggaggcg aagaacagcg gcattgatta ccgcctggtc aagctgccca
     4861 tggcagccgt gctgcggacg cggaccatct ccgagccgcg cggattcata aaaatgctcc
     4921 tcgacgtggc gagcgaccgg attctcggga tcacggttct cggcgccgag gcgagtgaac
     4981 tgatggcggt ggtgcagacc gcaatgctcg gagagcttcc ctatacggtg ctgcgtgatg
     5041 ccattttcac tcacccgaca gcagccgaag ggctcaacgt gctcctggca gacgtcgccc
     5101 cgaaggcgat gcacgaattc gctacctcat aagggtagga gtgatgttga gtcaatgcgt
     5161 tccagagcct gttatgaatt tccctttgga aacacaagga ggtaggtcat gcgagcagtg
     5221 agatttgctg gagtaggcaa gtccgcccag atcgcggatg ttgccaagcc gtcgcccgct
     5281 cccggtcagg ttttgataaa gatcggcggc gcgggcgtgt gtcattcgga tttgcacgtg
     5341 atggaagagg atttaggctt cacgcctccg ttcacgctcg gacatgagaa cgccggatgg
     5401 gtggcggagc ttggagaagg cgtcacaggt tttaaggaag gcgacgcggt cgccgtttat
     5461 ggtccgtggg gctgcggtcg ctgtcatgct tgccagctct cgatggagaa ctactgcgag
     5521 aactgggctg atctcggcgg cttcggcggc ggtctcggtt tcgacggcgg aatggcggag
     5581 tattttctcg tacccgcggc gcgtttgtta gttccgctcg aaggattgag tccggcgaaa
     5641 gccgcgcctt tgagcgacgc cgcgctgact ccgtatcacg ccatcaagcg cgccctgccg
     5701 cgactcaacg ccgatgatac ggtcgtcgtg atgggcgtcg gcggactcgg acacatggcg
     5761 attcaactcc tgcgtgtgct cgcgcccgtg cgcatcatcg ccgccgacgt ggacgagaag
     5821 aagctggagc aggcgaaggc gctcggcgcg gacgaagtcg tcaacaaccg cgacgccgac
     5881 gcggccgccg aacggatatt gaaactcacg gggacgcgcg gcgcgggctt ggtgcttgat
     5941 tgtgtcggcg tgcaaccgac gattgatctc ggtgcgaaac ttttgggacg caactgcgtc
     6001 tggacaatag tcggcttggg cggcgggcat catgactttc atcacggcag cacgccttac
     6061 ggctgctcga tgagcattcc gtattggggg tcgcgcgtcg aattgatgga ggtgattgcg
     6121 atggcgcgcg acggacgaat ccacgcggag acaaccgagt tccctttgga gcaggcagtc
     6181 gaagtctatg acaaactcaa agcggggcaa atcacgggtc gtgccgtgct gataccggat
     6241 ggcgcatcgc gttgatacat tgagcctgag ggagagtcct caatgtcttc aatcaacgag
     6301 gttcttgccg gtctgcaagc ggtctccgca tggcaggaac gtgtttacaa ggacttccat
     6361 gcgcatcccg agctgagttt tcaggaaacc aaaacagccg cgctcgcggc gtcgaaactc
     6421 agggaactcg actacgaagt ccacgaaggg attggtcgca ccggcgtcgt cggcatcctt
     6481 cgaaacggcg aaggccagac cgtgctggca cgggccgaca tggacgcgct ccccgtgcgg
     6541 gagaacaccg gtctgcctta tgcttcgaca ctcactgcgg ctgatatgga cggggaaacg
     6601 gtcagcgtcg ctcacgcctg cggccatgat gtgcacgtga cctgtctgtt gggcgcggcg
     6661 gaactgctgg ctaaagctcg taggtcttgg ggcggcacct tcgttgcgtt gttccaaccc
     6721 gccgaagagg tcgccggtgg cgcaaaggcg atggtggagg acggcttgcc agaccggatt
     6781 cccaaaccgg atgtcgcgtt tgcgcagcac gtgctggcct acccggcggg aacaatcggc
     6841 actcaggcgg ggcccgtact ttccgccggg gatagcattc gcctcaccct gcacggcaag
     6901 gggtcgcatg ggtctatgcc acacaacagc gtggacgcgg tggtgctggc cgcaatggtg
     6961 gtcgtccgcc tccagacgat tgtgtcgcgg gagacgaagc ccggcgagtt tgcggtgctg
     7021 accgtgggca gcagtgtggc cggctctaag agcaatatta tcccggatcg cgcgcagctc
     7081 ctggtcaatc tgcggaccta cgacacggac gtgcgccggc gcatgctcga cagcatcgag
     7141 cgcatggtca gggccgagtg cgaggcttcg gggtcgccgc gcccgcccga gttcgagtat
     7201 tacgatcagt atccgctgac cgacaatgac tcggaggtga cggaaaaaat cactcgcgca
     7261 ttcaaggatt atttcggagc ggaaacatta ttcgacctcg gacggcagac cgccagcgag
     7321 gatttcagcc tcatccccga cgccttcggg acgccctaca cctactgggg gatcggcggc
     7381 acggacccgg agcaatacga gcgcgctcac aaggccggtc gggtagagca ggacatcccg
     7441 gtcaaccatt cggaattctt tgcgccggtc attgaaccga cgctgtccgc cggcacgcgg
     7501 gccttggtgg ttgccgcttt gacgtacctg tcgaagtgag gaaggagggc accgggattg
     7561 atccagctcc cgggaagtga agaggcgctg agggccttag cagagttcaa ggcgtacgcg
     7621 gcgggcacgc ttctggtgct cgaccttgta ggcacgttcg tgttcgctct gagcggcgcg
     7681 gtggccggcg ttaaggaacg tctcgatctg ttcggagtgc ttgtgctttc gtttgcggcg
     7741 gccagcgccg gtggcatcat gcgcgacctg ctgatcggcg cggtcccgcc ggcggcaatc
     7801 agcgactggc gttacctcgg agtctctctc ctcgccgggt tggtcatatt cttctggttt
     7861 ccacgtagtg agcggctgca aaagctgcgc aacctcgtgc tggttttcga cggcgccggc
     7921 ctggcggtct tcgccgttgc cggcacgcaa aaggcgctcg gctacggtct caacccgttg
     7981 atgtccgcgc tgctcggcat gctgaccggg atcggcggcg gaatgctgcg cgacgcactc
     8041 ctcgcccaag tccctaccgt tttacgttcc gagctttatg ccgtagccgc actcgctggc
     8101 gcgtccgtgg tggtagtagg ccatgtgctg aacttcccgc ctaccgcgat ggcaatcgcc
     8161 ggcgcagtcc tctgcttcgg gattcgcctc gttgccatac ggcgcggctg gcgcctcccc
     8221 gtcgccgacc tgccgcaacg gtttgaggcg ggggcgggcg gcgcggacga tcagcaggac
     8281 aaggtaggga agttctaagg tgtgtgcagt tgtttgtatt aaagcataag gtaacgaaag
     8341 ccatcattct cgctaccgat tggagccacg gatgaactca gacgcagcgc aaaagcaacc
     8401 tgacacggcg gagggcgccg tcgaaatggt catcgagacg cgggcgcgcg acctcgtcga
     8461 aggctttaag gtccggcgcg cccttccctc aagaggaagg cgcatggtcg gccctttcat
     8521 cttcttagac cagatggggc ccgaagtttt aagggccggc aaagggctgg acgtggcgcc
     8581 gcacccgcac atcggtctcg ccaccgtgac ctacctcttc gcgggcgagc tgctgcaccg
     8641 cgacagcctc ggcaccgtcc agccgatccg ccccggcgag gtcaactgga tgaccgcggg
     8701 ccgcggtatc gcgcactccg agcgcacgcc gcaagagatg agactgtcga gttctgaact
     8761 cttcggcata cagtcatggg tcgccttccc cacgcaccat gaggagaccg aaccggcctt
     8821 cgctcaccac ggggcgtcgg agcttccggt catcgaaggc gagggcaagc tcgtgcgcct
     8881 catctgcggt tcgctctacg gcgctcgctc gcccgtgcag acattctctg agatgttcta
     8941 cgcggacatc acactggagg gcggcgcgcg gctccccgtc cccgccgagc acgaggagcg
     9001 cgcggcctac gtcgtcgaag gctcgatcgg actcatcccc gggggaggga cattccacgc
     9061 cgggcagctt ctcatcttta agccggggga ggagataaac ttgggcgcgc ctgagtcgtc
     9121 tcccgcccgc gtgatgctgc tgggcggtga gcctttggac gggaagcgtc acatctggtg
     9181 gaacttcgtc tccagttcgc gcgagcgcat cgagcaggcg aaggaggatt ggaaggcggg
     9241 acggttctcg cccgtccccg aagagacgga gttcatcccg ctgccggaga ccgggccagc
     9301 cgtggtgcgg tatccgtaag atataaacaa agccgggagg caacgtcgct ctgccgcgtg
     9361 caaaaaaatg atgacgagga ttcctgaaaa cgaagcatcc cgtcatcaaa tcgtttgagc
     9421 cgggcgagga gtggggctat tgctacgtcg atcagatttt cttcgagtcg ctctcaatcc
     9481 atagagcaca tccagccaat aacaatgaga gagtgagcaa tgcaaccaat aaccaaagaa
     9541 aagtcagtga agaagctccg ccgcgtccag cgcagtacgg agcgtcactg ggtcggcgac
     9601 gggttccccg tgcggacgct ctttgcctac tcggaccggg gggcggaact cagcccgttc
     9661 ctgctcctcg attacgccag cccaatggaa ttcccgccca cggataaacg cctcggcgtc
     9721 ggcgagcacc cgcaccgcgg cttcgagacg gtgaccatcg tctacgaggg cgaagtcgag
     9781 cacagggatt cctccggcgg cggcgggcgg atcggccccg gcgacgtgca gtggatgacc
     9841 gccgcgggcg gcatcgtgca tgaagagttt cacgggcgcg acttcgcccg gcgcggcggg
     9901 gtgttcgaga tggtgcaact ctgggtcaat ttacccgccg agaacaagat gtcgccgccg
     9961 ggttatcagg ccgtcctgaa tagcgagatt cccgccgtca gcctgcctga tgggcagggg
    10021 acggtgcggg tcatcgccgg cgagttcgcg ggagctaagg ggccggccaa gaccttcacg
    10081 ccgatgcacg tctgggacct gcgcctcgcc agcggccgac aggcagagct agcagtaccc
    10141 gatggctata cgacggcgct cgtggtgctc aaaggtgcgt tgcgtgtgag cggctccgag
    10201 gcagtcgagg cgtcggaggt cggactgttc gaccgggagg gacagagcat ccatctcgaa
    10261 tgcgtggaag acacgacggc gctgctgctt tgcggcgagc cgattgacga acccattgtg
    10321 gggcaggggc cgttcgtgat gaacacttcc caggagatcc gccaggcgat ggcggattac
    10381 tggagcggca agatgggcca cttgtcgtga ccggtgggaa ataacccccg cgcaggagga
    10441 gcagacgcta tgacaagaat cttgggaata tcgggaagcc tgcgccgcgg ctcttacaac
    10501 tcggcgctgc tgagggccgc cgtgcccttg ctggacgcgg gcgtggagct ggagatcgcc
    10561 tcactccacg gcatcccgct ttatgacggc gacctggaag ccgccgaggg cctgccgcag
    10621 gcggtgcgcg acctcaaggc gcgtgttgtg gcctgcgacg gattgctctt ggcgacgccc
    10681 gaatacaaca acggtattcc cggcgtgttc aagaacgcga ttgactggtt gtcgcggccc
    10741 ccgtccgaca tcccgcaggt gtttggtagc cgcccggtgg ctgtcgtcgg cgcatcaccc
    10801 ggcggcttcg gcacaattct ggcgcagaac gcttggctgc cggtgctgca cgcgctcggg
    10861 acgcgcccgt ggttcggcgg gcggctgctg gtgtcgcgcg cttctcacgt ctttaacgat
    10921 acaggcgaga tggtggacga agccatgcgc gcgcggttac gcgacttcct gcgtggattc
    10981 gcccagttca ttcaggccag caccgcgccg cagtaaggga ataaatctga gcgcggaaaa
    11041 tttgtgctga cggtcatttg agccgaacgc tcggccactc tcaagaagag aaaggagact
    11101 gaaatgaaca gccaagccac ttcccagtcg ccgcccatat cagaaatggc gggcatcaag
    11161 tttattgcgg tcgagagctt cagagagcag atagatgagt ttgctcaggt gctagaggct
    11221 cgcgcctacg agatatacga aaggcgcggg cgcgaagacg ggcacgcgct tgaggattgg
    11281 agccgcgccg aagcagaact gcgttgcatg gttccggtcg ggattctcga aggccctgag
    11341 cagttggaag tcaacatccg attggtcgga tgtctctgcc atgagatcga ggcctgcctc
    11401 gaaccgcgcc gcctcttgct ccgggcgcag aaagaagagg aagaaagtca aaagccgggg
    11461 gagaatgacg cggagcagcg tttgaatggc atcttccttg cgctcaacct gcccgaccag
    11521 gttgacccgg ccaaagtaac ggccgtcttc aaagacgaga acctctgcct gacgctgccg
    11581 agagctttcg cctgaggcgc aggggcgcgg attagggcga gctggtctca ccaagcggga
    11641 agttgtactc aacgatgaca gagctgcttc tggacagttc tggttaaccc ttgtgatgct
    11701 tcagtcatgt cgcctgcgcc gccggcgctt caaaacccat gcgtatgagt tatggtcgcc
    11761 cggtgtgacg tgccgtgggc gttttccgaa gtatgctgtc gctctagcct gcgctctcgc
    11821 ggtcgcagcc ctgttcaggc cgcaagcttc tgccgcgacc gaagctttac agcaaggagc
    11881 gcggatcgag ggcgaggtgg tagacgaatt cggcgcgccc gtcgcgggcg cgcaagtatc
    11941 gctcatcgtc aaagagtccg tcgtcgccca caccgcgacc ggcgaagacg gaagattctt
    12001 tcttaatacg gtgaccgtcg ccgaaggcac gctcgtcttc agcgcgcacg gctttgcgga
    12061 gatcaagcga gagtggagta cggcaaatcg agaggcgaaa gggctgcgaa tcgtgcttgc
    12121 gcctgctcct ctggcggaac aagtcgtggt gacggcgacg cgcacggaag tgcgcctcgg
    12181 cgacacggcg gcgagcgtca cgctcgtctc gtcagaagaa cttggggcga ccgccgcgct
    12241 cagactcgac gacgcgctca ggcagattcc gggcttccag ttgttccgcc gctcggggag
    12301 ccgcacggcg aatccgacct cgcagggcgt ctccctgcg
//
LOCUS       DASVES010000328         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_993739, whole genome shotgun sequence.
ACCESSION   DASVES010000328 DASVES010000000
VERSION     DASVES010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993739"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VF240_13555"
     CDS             complement(<1..1270)
                     /locus_tag="VF240_13555"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX7176283.1"
                     /translation="MEITFIFVLVLALALVALWRRTASAGSLAPRRAALTLALAVGAA
                     YFASGALAQAPRATPNQARGAGAAAESAPRAGGEANLILPDLASVDFLGGTNGRTLLM
                     FGLVVCALGLGFGLWIYTQLKNLPVHRAMREISELIYETCKTYLLTQGRFILILWVFI
                     GIITGVYFGFLAESYNEATRTIEHGFPPLKVATILLFSLVGIAGSYGVAWFGIRVNTF
                     ANSRTAFASLQGRPYPIYAIPLKAGMSIGMMLISVELLIMLCILLFVPRDYAGPCFIG
                     FAIGESLGAAALRIAGGIFTKIADIGADLMKIVFKIDEDDPRNPGVIADCTGDNAGDS
                     VGPSADGFETYGVTGVALITFILLAVPSATVQVQLLVWIFVMRVMMVITSIAAYAVNQ
                     ARARSKYADADEMDFEAPLTWLVWITSVISV"
     gene            complement(1597..2130)
                     /locus_tag="VF240_13560"
     CDS             complement(1597..2130)
                     /locus_tag="VF240_13560"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX7176284.1"
                     /translation="MFRMFALSVVLVFFAFTPAAHARRPQGAPTVVILVRHGEKEKEP
                     TGDPPLSELGRKTAERLAVIGEGAGVEVVFTSQAKRTRDTAAPLAARRGLTPNVRNVT
                     RETVDAHVRGLAKEILDSHRGKTVLVVGHSNTAPRIAALLSGKTIPDLDDATEFDAIF
                     VVIIPESGAPKLVRAKY"
BASE COUNT          413 a          699 c          822 g          374 t
ORIGIN      
        1 cgaccgaaat aaccgaggtg atccaaacga gccaggtcag cggcgcctcg aaatccattt
       61 catccgcgtc cgcatatttg ctccgcgccc gcgcttgatt gacggcatac gccgcgatgc
      121 tcgtaatcac catcatcacg cgcatgacga aaatccacac cagcagttgc acctgcacgg
      181 tcgcgctcgg cacggccagc aggatgaagg tgatgagcgc cacgcccgtc acgccgtagg
      241 tctcgaagcc gtcggccgaa ggcccgacgg agtcgcccgc gttgtcgccc gtgcagtcgg
      301 cgatgacgcc cggatttcgc ggatcgtctt cgtcaatttt gaagacgatc ttcatgaggt
      361 cggcgccgat gtcggcgatt ttcgtgaaga tgccgcccgc gatgcgcagc gccgccgcgc
      421 ccagactctc gccgatggcg aagccgatga agcaggggcc ggcgtagtcg cgcgggacga
      481 acagcaggat gcagagcatg atgagcagct cgacggagat gagcatcatg ccgatggaca
      541 tgccggcctt caaggggatg gcgtagatcg ggtacgggcg gccctgtagg ctggcgaagg
      601 cggtgcgcga gttggcgaag gtgttgacgc ggatgccgaa ccacgccacc ccgtagctgc
      661 cggcgatgcc gacgaggctg aagaggagga tggtggcgac tttgagcggc gggaagccgt
      721 gctcgatggt gcgcgtcgcc tcgttgtagg actcggcgag aaagccgaag tagacgcccg
      781 tgatgatgcc gatgaagacc cacaggataa ggatgaatcg cccctgcgtg agcaggtagg
      841 tcttacacgt ctcgtaaatc agctcggaga tttcgcgcat cgcgcggtgg acgggcaggt
      901 tcttgagctg cgtgtaaatc cagaggccga agccgaggcc gagcgcgcaa accacgaggc
      961 cgaacatgag gagcgtgcgc ccgttcgtgc cgcctagaaa atcgaccgac gccagatcgg
     1021 gcaggatgag gttggcctca ccgcccgcgc gcggcgcgga ctcggcggcg gctcccgcgc
     1081 cacgcgcctg attgggtgtg gcgcgcggcg cctgcgcgag cgcgcccgag gcgaagtaag
     1141 cggcgccgac ggcgagcgcg agtgtgagcg cggcgcgacg cggcgcaagc gagccggccg
     1201 aggcggtgcg tcgccagagg gcgacgagcg cgagcgcgag gacgagaaca aagatgaacg
     1261 tgatttccac ggttaagaca aatcctttct gttcgtttaa tagcaggcga gataaaactt
     1321 tgccttcaaa ggggcgcaag tctcaaccag agcggcgtct gggcggacgg aggccacgag
     1381 aggggagcct cgggtccgca ctgaccggcc ggttgggtct cgcttaaagc gggaattatt
     1441 gccgatttat gctcgtactg tccacccgcc agtcgcaggt caggagaaag attggttcat
     1501 tacagtaccg cgagcggtag cgagcgggta cgcgtgcgat gagaggcccg catcgcgcat
     1561 cacgacccgc tcgctaccgc tcgcggtact gttcgttcaa tacttcgccc tgacgagctt
     1621 cggcgctccc gactcgggga tgatgacgac gaagatggcg tcgaactcgg tggcgtcgtc
     1681 gaggtcgggg atggtcttgc cgctcaggag ggcggcgatg cgcggcgcgg tgttgctgtg
     1741 tccgacgacg agcacggtct tcccccggtg gctgtccaga atctcctttg ccagccccct
     1801 gacgtgcgcg tccacggtct cgcgcgtgac gttgcgcacg ttcggggtga ggccgcggcg
     1861 cgcggccagc ggcgcggccg tgtcgcgcgt gcgtttggcc tgcgacgtga agacgacctc
     1921 gacgcccgcc ccctcgccga tgacggcgag cctctcggcg gtcttgcgtc caagctcgct
     1981 gagcggcggg tcgccggttg gctccttctc cttctcgccg tggcgcacga ggatgacgac
     2041 tgtgggcgcc ccctgcggcc gacgggcatg cgcggccggc gtgaacgcga agaagacgag
     2101 gacgactgac agggcgaaca ttctgaacat gatcggtttc cttagattcg tttacgcagt
     2161 tcgacggcgg ccgcgctact cgaggctcgc cgccgggtgc gccgggacga gcgtgccgcc
     2221 gcgccttcgg tcgccgcgca cgcgaaggat ggcgagcacc gtgaggtaga gcgcgaggcc
     2281 gacgagggcg gcggccgtga gcgcgttg
//
LOCUS       DASVES010000329         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1569113, whole genome shotgun sequence.
ACCESSION   DASVES010000329 DASVES010000000
VERSION     DASVES010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569113"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1016)
                     /locus_tag="VF240_13565"
     CDS             complement(156..1016)
                     /locus_tag="VF240_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX7176285.1"
                     /translation="MRANLPQYELTAPRTLDDALGLLAREPGAWRPFAGGTDLMVLLE
                     AGRLAHKKFFSVFHLAELRGIEETPEWMSLGALTTYTDVRRNQTLRSEFPMLGRAAAE
                     TGGLAIQNRGTLGGNIANASPAADTPPALLAYGAEIELVSTSGTRRVPYADFHTGYKQ
                     TVMRPDELIARIHLPRPRPGAMHHYRKVGTRRAQAISKVCFAALAEMDEGGRVGEVRL
                     AFASVAPVVLRCRKTEAVLRGRRPDELVVGEAASELSREIVPIDDVRSTANYRRRVAV
                     NLLKDFLSKI"
     gene            complement(1013..1516)
                     /locus_tag="VF240_13570"
     CDS             complement(1013..1516)
                     /locus_tag="VF240_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7176286.1"
                     /translation="MATEKITVSFKVNGREETAEVYPMARLLDVLRESLGLTGTKEGC
                     GEGECGACSVLIDGELVNSCLVPVAQITGAEVLTIEGVATGPETLHAVQAAFITHGGA
                     QCGICTPGMIMAAVKLLEEKPRPTEADIREGLAGNLCRCTGYVKIFESVVRAVGAGER
                     EGARGEG"
     gene            complement(1546..>3480)
                     /locus_tag="VF240_13575"
     CDS             complement(1546..>3480)
                     /locus_tag="VF240_13575"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7176287.1"
                     /translation="AHEDKYLLEEARRSVRVEVEPLPAVFTIEESLQKKEIIWGEDNI
                     FKSYLVDKGDVDAVWGRADFVVEGEYRTGAQEQLYIETNGCIAQATPEAGVTVWGSMQ
                     CPYYVHKALVKLFALPDDKIRVVQTETGGGFGGKEEYPSMIAGHAALLAWKAGRPVKI
                     VYDRAEDMAATTKRHPSITRHRTAVTREGRLLGMEIDFDVDGGAYATLSSVVLSRGTI
                     HAAGPYNCPNVRIRSRAVATNAPPHGAFRGFGAPQSIFALERHMDKVARAVGLSPEEL
                     RRRNFIRRGETTATGQIIHEDVDMEGLLERAFDLSDYRAKSERFVAENARREDVKRGI
                     GFATFMHGAGFTGSGEKFLQSVVGCEATPEGRVRVLAASTEIGQGTNTIFAQIAAEAL
                     ALDPDTIEIGRPDTGNVPNSGPTVASRTCMIVGKLVESAALGLKKQLVGAGMLGDTYT
                     GDEFQSACRAFLERHGALKSVSTYTQPDDIVWDDEHYRGDAYGAYAWAVYVAEVSVDM
                     LTYEARVEDFVAVQEVGRVINPVLAAGQIEGGVAQGVGFALFEKVAWQDGRMMNGQMT
                     NYIIPTAADMPPVRVHFYEHPYGGGPGGAKGIGELPMDGPAPAILNAIENATGCAFNH
                     APLMPEDLMEKLAAVEASVL"
BASE COUNT          529 a         1191 c         1137 g          623 t
ORIGIN      
        1 gggagggcgc gagcgtccat gcgccctccc tcacggtcgg gctactgccc tgccctgcgc
       61 cgagaactca tccacttcat cccccccatc catgttaatt gcctcccacg atgcgggcag
      121 ggacaagccc tgcccctaca aacatatcaa aggcttcaaa tcttcgacag aaagtctttc
      181 aaaaggttca ccgcgacgcg ccgccggtag ttggccgtcg agcgcacatc gtcgatgggc
      241 actatctccc gcgaaagctc cgaggcggcc tcgcccacaa ccaactcgtc cggcctccga
      301 ccccgaagaa cggcctccgt cttccggcag cgcagcacga ccggcgcgac actcgcgaag
      361 gccagacgca cctcgccgac gcgcccgcct tcgtccatct ccgcgagcgc ggcgaagcag
      421 actttcgaga tggcctgcgc gcggcgcgtg ccgaccttgc ggtagtggtg catggcgccg
      481 gggcgcgggc gcggcaggtg gatgcgcgcg atgagttcgt cggggcgcat gacggtttgc
      541 ttgtagccgg tgtgaaaatc ggcgtagggg acgcggcgcg tgccggaggt ggagacgagt
      601 tcgatctcgg cgccgtaggc gagcagggcc gggggcgtgt cggcggcggg cgaagcgttg
      661 gcgatgttgc cgccgagcgt gccgcggttc tggatggcga ggccgcccgt ttcggccgcc
      721 gcacggccga gcattggaaa ctcgcttcgc agcgtctggt ttcggcgcac gtcggtgtac
      781 gtcgtcagcg ccccgaggct catccactcc ggcgtctcct cgatgccgcg cagctccgcg
      841 agatggaaga cgctgaagaa ttttttgtgc gcgagtcgtc cggcttcgag cagcaccatc
      901 aggtctgtgc cgccggcgaa gggccgccac gcgcccggct cgcgcgcgag cagccccagc
      961 gcgtcgtcca gcgtgcgcgg cgctgtcagc tcgtattgcg gcaggttcgc cctcatcctt
     1021 cgcctctcgc accttctctt tcgcctgcgc caactgcgcg caccaccgac tcgaatattt
     1081 tcacgtagcc ggtgcaacgg cagagattcc ccgccagtcc ctcgcgaatg tcggcctcgg
     1141 tcggcctcgg cttctcttcc agaagtttca cggcggccat gatcatgccc ggcgtgcaga
     1201 tgccgcactg cgcgccgccg tgcgtgatga atgccgcttg cacggcgtgc agggtctccg
     1261 gccccgtcgc gacgccctcg atggtcaaca cctccgcgcc cgtgatctgc gccacgggca
     1321 cgagacaact gttgaccagc tccccatcga tgagcacgga gcacgcgccg cactcgccct
     1381 cgccgcagcc ttctttcgtg cccgtcagac ccagcgactc gcgcagcacg tcgagcagac
     1441 gcgccatcgg gtagacctcc gcggtctcct cgcggccgtt cactttgaag ctgactgtga
     1501 ttttttccgt cgccactaac tcacttcatc tcccgccgag gcgcttcaga ggacggacgc
     1561 ctcgaccgcc gccagcttct ccatcaagtc ttcgggcatg agtggggcgt ggttgaaggc
     1621 acagcctgtc gcgttctcga tggcgttgag gatggccggc gcagggccgt ccatcggaag
     1681 ctcgccgatg cccttcgccc cgccggggcc gcccccatat gggtgctcgt agaagtggac
     1741 gcgaacgggc ggcatgtcgg cggccgtcgg gatgatgtag ttggtcatct gcccgttcat
     1801 catccggccg tcctgccacg cgactttctc aaacaaggcg aagccgacgc cctgcgccac
     1861 gccgccctcg atctgcccgg ccgcgagcac tggattgatg acccggccca cttcctgcac
     1921 ggcgacgaag tcttcgacgc gcgcctcata cgtaagcatg tcaacggaca cctcggcgac
     1981 gtagacggcc cacgcgtaag cgccgtaggc gtcgccgcga taatgctcgt cgtcccagac
     2041 gatgtcgtcg ggctgtgtgt aggtgctcac cgacttcagc gcgccgtgtc gctcaaggaa
     2101 ggcgcggcag gcgctttgga actcgtcccc cgtgtaggtg tcgccgagca tgccggcgcc
     2161 gacgagttgc tttttcaatc cgagcgcggc cgactcgacg agcttgccga cgatcatgca
     2221 cgtgcgcgag gcgacggtcg ggccgctgtt gggaacgttg ccggtatcgg ggcgtccgat
     2281 ctcgattgtg tcggggtcga gcgccagcgc ctcggcggca atctgcgcga agatggtgtt
     2341 tgtgccctga ccgatctcgg tgctcgccgc gagcacgcgc acacgcccct cgggcgtggc
     2401 ctcgcagccg acgaccgact ggaggaactt ctcgcccgag ccggtgaagc ccgcgccgtg
     2461 catgaacgtg gcgaaaccga tgccgcgctt cacgtcttcc cgccgcgcgt tctccgcgac
     2521 gaacctctca ctctttgcgc ggtagtcgct cagatcgaag gcgcgttcca gcagcccctc
     2581 catgtcaacg tcttcgtgga tgatctgccc cgtggcggtc gtctcgccgc ggcggatgaa
     2641 gttgcgacgg cgcagctcct cgggcgagag gccgaccgcg cgggcgactt tatccatgtg
     2701 gcgttcgagc gcgaagatgc tctggggcgc gccgaagccg cggaaggctc cgtggggcgg
     2761 ggcgttggtg gcgacggcgc gggagcggat gcggacgttg gggcagttgt aggggccggc
     2821 agcgtggatg gtgccgcgcg acagcaccac cgaggagagc gtggcgtaag cgccgccgtc
     2881 cacgtcgaag tcgatctcca tgccgagcag acgcccttcg cgcgtgacgg ccgtgcggtg
     2941 tcgcgtgatg gacgggtggc gcttggtcgt ggccgccatg tcttcggcgc ggtcgtagac
     3001 aatcttgacg gggcgacccg ctttccacgc gagcagggcg gcgtggccgg cgatcatcga
     3061 gggatattcc tctttgccgc cgaagccgcc gcccgtctcg gtctggacga cgcgaatctt
     3121 atcgtcgggc agcgcgaaga gtttcaccag agccttgtgg acgtagtagg gacactgcat
     3181 cgacccccac acggtgacac ccgcctcggg cgtggcctgt gcgatacagc cgttggtctc
     3241 gatgtagagc tgctcctgcg cgcccgtgcg gtactcgccc tcgacgacga agtccgcccg
     3301 cccccacacc gcgtccacgt cgcccttatc gacgagatac gacttgaaga tattgtcctc
     3361 tccccagatg atctctttct tctgaagcga ctcctcgatg gtgaagacgg cgggcagcgg
     3421 ctcgacctcg acacgcacgg agcggcgcgc ctcttcgagc agatacttgt cttcgtgcgc
//
LOCUS       DASVES010000330         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_109571, whole genome shotgun sequence.
ACCESSION   DASVES010000330 DASVES010000000
VERSION     DASVES010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109571"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /gene="pheT"
                     /locus_tag="VF240_13580"
     CDS             complement(<1..1650)
                     /gene="pheT"
                     /locus_tag="VF240_13580"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7176288.1"
                     /translation="MNISYNWLRELVDVSLAPRELAARLTMLGLAVDAVHEAGDDHVL
                     EIDLTSNRPDCLSHVGVAREAAALEGKSVHLPKTEAARTGGRTEEFASVSIEDEELCP
                     RYAARVVRGVKIAPSPAWLVERLQAIGQRPINNVTDITNFVLHEMGQPLHAFDLATLA
                     GRRIVVRRARSGEKLRTLDGAERELDEQMLVIADAERAVALAGVMGGEETEITEATAD
                     VLVESAYFAPASVRRTSKQLGLQTEASYRFERGTDYEGVRRAQDRCVALICEIAGGEA
                     AKDAIDVYPRRPAPVSVSLRHERVGKLTGLDVSAEESERILTALGFKRDGAGEAAPGI
                     EEVAAGDSAARFVVPSWRADVSLEEDLVEEVARHFGYEKIKDELPASGIAGEYRRGEE
                     RRRAAKRALNALGFDEAISFSFIDTSGEGAFERLPSLQPPAGAGGAEFVYLSNPIIEG
                     AALMRPTLLPGLLGAVRHNLNHGTRNLRLFEAGRVFAAAGGGAGQPVEREALALVLAG
                     AALEEGRAGAARELDFYDLKGALEAASGGAGVTGLEFEPASV"
     gene            complement(1736..2833)
                     /locus_tag="VF240_13585"
     CDS             complement(1736..2833)
                     /locus_tag="VF240_13585"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7176289.1"
                     /translation="MSEVGEHQSQIDSVREAFEQEFGRFARFRSGGDAGAGLSLGDAE
                     AALRDLADLRTRHTGKKSALSQLMKLVGRVAADERADFGQRVQSLRAEVESALDEAEQ
                     SLRAHAAAERIERESIDVTLPGRRPRRGHLHPVTIVRQRLEDIFVAMGYTVEDGPEIE
                     NDYYNFTALNIPELHPARSPQDTFYTTDGFALRSQTSTVQIHAMEKRRPPLRVIAPGR
                     VFRRDTPDATHNPMFFQLEGLLVDRGIHMGHLKGTVTEFLRRLFGSDVRTRFRPSYFP
                     FTEPSAEFDFSCFKCGGSGCRLCKGSGWIELGGSGMVHPNVLRGVGIDPDEFSGFAFG
                     FGIDRMCALLYELDDIRLLFENDVRFLEQFE"
     gene            complement(2862..3236)
                     /gene="rplT"
                     /locus_tag="VF240_13590"
     CDS             complement(2862..3236)
                     /gene="rplT"
                     /locus_tag="VF240_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7176290.1"
                     /translation="MPRAKRGNKRTEKRKKILELAKGYRGTKSKLYRQAKESVERGLK
                     FAYTGRKLKKRDFRSLWIVRIGAAARINGLNYSQFMHGLKTAGIELDRKVLADIAANS
                     PDVFAELAGQAKNAIDNRPAAA"
     gene            complement(3345..3548)
                     /gene="rpmI"
                     /locus_tag="VF240_13595"
     CDS             complement(3345..3548)
                     /gene="rpmI"
                     /locus_tag="VF240_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7176291.1"
                     /translation="MPKLKTHKGAAKRFRTTATGKVKRGHSHARHILTSKSTKRKRLL
                     DIDALVSESDEVRIKRMLPYGRS"
     gene            complement(3618..>4074)
                     /gene="infC"
                     /locus_tag="VF240_13600"
     CDS             complement(3618..>4074)
                     /gene="infC"
                     /locus_tag="VF240_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX7176292.1"
                     /translation="FRPGTDDHDYNFKMKHAHQWLDEGDKVKATIFFRGREITHRELG
                     AKLLERLEKDLAPVGEVEQRPRMEGNQMFFIFTPKRHKTSKHEGAKAEAAAKPKPAAP
                     PPQPVEPTPPAQPTADAQPEPPAQPAPPTQPAPPVPATAATPEGGGQPE"
BASE COUNT          645 a         1340 c         1336 g          753 t
ORIGIN      
        1 cacgctcgcg ggttcaaact caagccccgt gacgcccgcc ccacctgaag ccgcttctag
       61 cgcgcccttc aaatcataaa aatctaactc gcgcgccgcg ccggcccttc cctcctccaa
      121 cgccgcgccc gccaacacga gcgccagcgc ctcgcgctcc acaggctgcc ccgcgccgcc
      181 cccggccgcg gcgaagacgc gtcccgcctc gaatagtcgc aggttgcgcg tgccgtggtt
      241 gaggttgtgg cgcacggctc cgagtaagcc cggcaaaagc gtcgggcgca tcagcgccgc
      301 gccctcgatg atcgggttcg acaggtagac gaactcagcg ccgcctgccc ccgcgggcgg
      361 ttgcaggctc ggcaaccgct cgaacgcgcc ttcgccggag gtgtcgatga agctgaagct
      421 gatggcctcg tcgaaaccga gggcgttcag cgcgcgcttg gccgcgcgcc tgcgctcctc
      481 gcccctgcgg tactcaccgg cgatgccgga cgcgggcagc tcatctttta tcttctcgta
      541 gccgaaatgt cgcgccacct cttcgactaa gtcttcctcc aacgacacgt cggcgcgcca
      601 cgaggggacg acgaagcgcg ccgccgagtc accagccgct acttcctcaa tacccggcgc
      661 cgcctcgccc gcgccgtcgc gcttgaaccc gagcgccgta agtatccgtt cggactcctc
      721 ggcggagaca tccagccccg tcaattttcc gacgcgctcg tggcgcaggg agacgctgac
      781 gggcgcaggc cgtcgcgggt aaacgtcaat cgcgtccttg gccgcctcgc cgcccgcgat
      841 ctcgcagatg agcgcgacac accgatcctg cgcgcggcgc acgccctcgt agtccgtacc
      901 gcgctcgaag cggtaggagg cttcggtctg aaggccgagc tgcttcgacg tgcggcgcac
      961 cgacgcaggc gcgaagtaag cgctctcgac gagcacgtcg gcagtcgcct ccgtgatctc
     1021 cgtctcttcg ccgcccatca ccccggcgag cgcgacggca cgctcggcgt ccgcgatgac
     1081 gagcatctgc tcgtcaagct ctcgctcggc gccgtcgaga gttcgcaact tctctcccga
     1141 gcgcgcgcgg cggacgacta tccgtcggcc ggcgagggtc gcgaggtcga aggcgtgaag
     1201 tggctgcccc atctcgtgga ggacgaagtt ggtgatgtcc gtgacgttgt tgatgggccg
     1261 ctgcccgatg gcctgcaagc gctcgacgag ccacgccggt gacggagcga tcttcacccc
     1321 gcgcacgacg cgcgccgcgt agcgcgggca caactcctcg tcctcgatgc tcacggacgc
     1381 gaactcctca gtccgcccgc ccgtccgcgc cgcttcagtc ttcggcaaat gcacggactt
     1441 gccttcgagc gccgccgcct cgcgcgcgac gcccacgtgc gacaaacagt cgggccggtt
     1501 cgacgtgagg tcgatctcca acacgtgatc gtcgcccgcc tcgtgtaccg cgtccaccgc
     1561 gaggccgagc atcgtgagtc gcgccgccag ctcgcgcggc gcgagcgaca cgtcaacgag
     1621 ttcgcggagc cagttgtagc tgatgttcat aacaaagggg tgataagtga tgagtgataa
     1681 gtgataagaa aagactattt gtttttctta tcacttatca ctcatcactt atcacttact
     1741 cgaactgttc gaggaagcga acgtcgttct cgaagaggag gcggatgtcg tccagctcgt
     1801 agagaagggc gcacatgcgg tcgatgccga agccgaaggc gaagccggag aactcgtccg
     1861 ggtctatccc gacgccgcgc agtacgttcg ggtgaaccat gccggagccg cccagctcga
     1921 tccagccgga gcctttgcag agacgacaac ctgagccgcc gcacttgaag caactgaagt
     1981 cgaactcggc gctcggctcg gtgaacggga agtaggaggg gcggaagcgc gtccgcacgt
     2041 cgctgccgaa gaggcggcgc aggaactccg tcaccgtgcc cttgaggtgc cccatgtgga
     2101 tgccgcgatc tacgaggagt ccttcgagct ggaagaacat ggggttgtgc gtcgcgtcgg
     2161 gcgtgtcgcg gcggaagacg cgccccggcg cgatgacgcg cagggggggc cggcgcttct
     2221 ccatcgcgtg aatctgcacg gtcgaggtct gcgagcgcag ggcgaagccg tcggtggtgt
     2281 agaaggtgtc ctgcgggctg cgggccgggt gcagctcggg gatgttgagc gcggtgaagt
     2341 tgtaatagtc gttctcgatc tcggggccgt cctcgaccgt gtagcccatc gcgacgaaga
     2401 tgtcttcgag tcgctgccga acgatagtga cggggtgcag gtggccgcga cgcgggcgtc
     2461 ggccgggcaa cgtcacgtcg atgctctcgc gctcgatgcg ctcggccgcg gcgtgcgcgc
     2521 gcagagactg ttccgcctcg tcgagcgccg actcgacttc ggccctcaaa ctctgaaccc
     2581 gctggccgaa gtcggcgcgc tcgtcggccg cgacgcgccc aaccagtttc atcaactgcg
     2641 agagtgcgct cttcttgccg gtgtggcgcg tgcgaaggtc agcgaggtcg cgcaaggcag
     2701 cttcggcgtc gccgagcgag aggcccgcgc cggcgtcgcc ccccgagcga aagcgcgcga
     2761 agcgtccgaa ctcttgctcg aacgcttcac gcaccgaatc gatctgtgat tggtgttcgc
     2821 cgacttcaga cactaacaaa gccctcatgg gcgactgtca tttacgcagc cgccggacga
     2881 ttatcaatgg cgttcttcgc ctggcccgcg agttcggcga acacgtcggg cgagttggcg
     2941 gcgatgtcgg cgagcacctt gcggtcgagt tcgatgccgg cggtcttgag gccgtgcatg
     3001 aactgcgagt agttcagccc gttgatgcgc gcggcggcgc cgatgcggac gatccacagg
     3061 ctgcggaagt cgcgcttctt gagcttgcgg ccggtgtaag cgaacttcag cccgcgctcg
     3121 accgactcct tggcctgtcg gtagagcttc gacttcgtcc cgcgatagcc tttcgccagc
     3181 tcgagtatct tcttgcgctt ctcggtgcgc ttatttccac gttttgccct gggcatgatt
     3241 cactcccttc aaagaaacag aggtcagagg tcagaggtca gaggttagtt aagaagcagg
     3301 tgtttgactg acctctaacc tctgacctct gacctctccg tttttcagct tcgcccgtag
     3361 ggcaacatgc gtttgatgcg aacttcgtcc gactcggaga ccagcgcgtc gatgtcgagg
     3421 agacgtttac gcttggtgga cttcgaggtc aggatgtggc gcgcgtgcga gtggccgcgc
     3481 ttgaccttcc ccgtggcggt cgtgcggaag cgcttggccg cgcccttgtg cgttttaagt
     3541 ttcggcatga ttacttctcc ctctcaagta tggcggtcgc cggccggacg gccggaaaac
     3601 cgcgcgtcga aaatttacta ctcgggctga ccgccgccct ccggcgtcgc cgccgtcgcc
     3661 ggcacgggcg gcgcaggttg cgtcggcggc gcaggctgtg cgggcggttc cggctgcgcg
     3721 tccgcggtcg gctgtgccgg cggcgttggc tctacgggct gcggcggcgg tgcggccggt
     3781 ttgggtttgg cggccgcttc ggccttggcg ccctcgtgct tcgaggtctt gtggcgcttg
     3841 ggggtgaaga tgaaaaacat ctggttgccc tccatgcgcg ggcgctgctc gacctcgccg
     3901 accggggcga ggtctttttc gagccgctcc aacagtttcg cgcccagctc gcggtgcgta
     3961 atctcgcgcc cgcggaagaa gatggtggct ttgaccttgt cgccctcgtc gagccactgg
     4021 tgcgcgtgct tcatcttgaa gttgtagtcg tgatcgtccg tgccggggcg aaac
//
LOCUS       DASVES010000331         7234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1181086, whole genome shotgun sequence.
ACCESSION   DASVES010000331 DASVES010000000
VERSION     DASVES010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181086"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF240_13605"
     CDS             <1..190
                     /locus_tag="VF240_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176293.1"
                     /translation="AVGAAAGAPFQTEAQEMKAQRGGVQEDQIAGEQAEGAHNQRRQN
                     RNPSGNDAEAGAEGGENF"
     gene            complement(358..4077)
                     /locus_tag="VF240_13610"
     CDS             complement(358..4077)
                     /locus_tag="VF240_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7176294.1"
                     /translation="MKIRTRQFASLVLCLVFIPLAAPPRAGTQRRARGGEQTGPTNTR
                     EEAYRANNLGVALLEQFRHADGAEQFRRALRLDPGLALARINLAVALFNVPDADAARR
                     EAEAAVALAPRAPQPHYILGLIARSQNRPDDALASFRRVLDADARDVGALVNTGQLLA
                     QQRKYPEAVEAFRAALSEEPYNGTALYNLGTALLRAGQREEGQRMIERFQALRQSGAA
                     TVIGPNYLEQGRYAEAVASTGAEPDLVDPTTPRVAFTDATASVMPQGRAAGSRRARRA
                     AAKPDATSPRAGERKRSLAAALGGGVTLFDFDSDGDLDLFEVTPTSQRLRRNDGGRLV
                     DVTSRSGALAEAAPGIGIGALAGDFNNDERPDLFVLRNGALNLYRNEGRGLFADVTAA
                     AGIPAYPHLSISAAFLDADHDGDLDIFVAGFAQSATDAARESQPGEARRTPVDAPNLL
                     LRNNGDGAFKDFTAEAGLSAKGNAVAVVPTDYDNRRDVDLLVVYHDKQPALYRNLRDG
                     TFRDVGTEAGLRFDATGLTCAAAGDVNKDGFTDFYFGRGAFPGYFAMSDGKGRFNVVD
                     GPAGRAESGMSAKSVERHAAQFLDYDNDGLLDLVAVAINDTSGEVGVWRNTGRGWADV
                     SEHATRGLFSAASDARPSPRVLVSGDVDADGDTDLVFRREGGPLVFARNEGGNRRGAV
                     RVRLAGKVSNRSGVGAKVEMRAGSLSQKHETYSAMPAPAPADVVFGLGGRARADAVRV
                     LWPAGIVQAETEISATGAAAVGRGRVGAVSSVSVTELDRKPSSCPYLYTWNGERFEFI
                     TDFLGGGELGYWLAPGVRSAPDTDEYVRVRGDQLKARGGRYEIRVTNELEEVLFLDRL
                     RLVAVDHPAGVEVYPNEGLGRPDGPALKLYATRSSRAPFAARDEHGHDVLARIAHMDR
                     RYPDDFALHRIRGYAEEHTLTLDLGRDERRDEGRGMRDDVKHGSSYSSLIPPPSPLRG
                     RTLLLLTGWTDYAFSSDNVAAAQSGMKLRPPALQVKDAGGRWQTVIEDIGVPVGRPQT
                     VVVDLTGKLPAGAREVRVVTNMRVYWDQVLLDTSGGDFPVKMTTLEPASAELRWRGFS
                     AEETPDGREPYGYDYARVSHTSPWKVMPGRYTREGDVLPLLLGGVDDMFVVARPGDEA
                     AISFDAAQLPALPVGWARTFLIYADGFSKEMDLNSSSPDTVAPLPFHGMKNYPYGHEE
                     SYPATPAHRDYIERYNTRIVAAPVSRMETMLTGVR"
     gene            4358..5377
                     /gene="rtcA"
                     /locus_tag="VF240_13615"
     CDS             4358..5377
                     /gene="rtcA"
                     /locus_tag="VF240_13615"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEX7176295.1"
                     /translation="MIIIDGSFGEGGGQIIRTSLALSLITGKPFRVMNVRARRDRPGL
                     QRQHLTAVNAAATVGAARVEGAAIGAREFTFVPGEVTAGEYSFPTGTAGSTTLVAQAI
                     LPPLMLAAAPSVVTLEGGTHNVHAPPFEFLEKTFVPLVNRTGAGVSVELVRYGFYPPG
                     GGRLDVLIEPRAVARRLDITELGGVRRAGARALVVKLAPSVGERALEVVRRKLGWEGD
                     ALRLELSQDALSPNVVLTLEVESEHLTEVFTGVGERGVRVETIAERVCDEAARYLEIG
                     APVGAHLADQLLIPLALSGGGSYITGPLSLHTTTNIEIVKKFLPVEITASHLSNELWK
                     VEVFA"
     gene            5470..5964
                     /locus_tag="VF240_13620"
     CDS             5470..5964
                     /locus_tag="VF240_13620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX7176296.1"
                     /translation="MTHDRPIKLYVFDADGTLRRTTVEGQPCPNKPGEWELIPGVRER
                     LSQIDWTCASFGVASNQGGVGTGYMRHEAAWQLLFDMAAAALGSEPPAGSIEMCPHAP
                     YSNCPCRKPKPLMLRRLMHRFRATKRETLFVGDMDRDKEAARRAGVRFLWAHEFFGWE
                     TPGA"
     gene            complement(5961..6710)
                     /locus_tag="VF240_13625"
     CDS             complement(5961..6710)
                     /locus_tag="VF240_13625"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX7176297.1"
                     /translation="MSNMKDPRPETLDRLALRTRPEGMPLMRQTWGKLLFMHWRIDRE
                     VLRPLIPESLSIDTFDGSAWIAVTPFTMWNVRLSFAPPVPGLSSLHELNVRTYVHVDG
                     VPGVWFFSLDANSRAAVVSARTFYQLPYFHAEIALEQEGETINYDLARTDDPPAEFHA
                     SWKTGGNSRFARPDTLEFFLVERYCLYALRGDRLMRARIHHEPWPLRDAELLTLDSTM
                     IESHGLPTPAGEPLLHYAEELDVDIWGPEEV"
     gene            complement(6837..7013)
                     /locus_tag="VF240_13630"
     CDS             complement(6837..7013)
                     /locus_tag="VF240_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176298.1"
                     /translation="MAKSKRQADPGESRAGKSPPSAARNDRPRKGGKRTPAEQGKPTV
                     GKQDVGRGDGKTLH"
BASE COUNT         1122 a         2344 c         2568 g         1200 t
ORIGIN      
        1 ggccgtcggc gcggcggccg gggcgccgtt ccagaccgag gcgcaggaga tgaaggcgca
       61 acgcggcggc gtgcaggagg atcagattgc cggcgaacag gccgagggcg cgcacaacca
      121 gcgccggcaa aaccgcaacc cttcgggcaa cgacgccgaa gccggcgcgg agggtggcga
      181 gaacttctag tcgccgccgg tgctcagggt tgagcatcat gcgggactga ttcaccgcgg
      241 aggcgcaaag gacgcggagt acacgcagag agattcaacc ctctgcgtga actctgcgtc
      301 ctccgcgcct ccgcggtgaa cgcttctcac gcctcactca actttaatac aaacctctca
      361 cctcacaccc gtcagcatcg tctccattct cgacaccggc gcggccacga tgcgcgtgtt
      421 gtaacgctcg atgtaatcgc ggtgcgccgg cgtcgccggg tagctctctt cgtggccgta
      481 ggggtagttc ttcatgccgt ggaacgggag cggcgcgacc gtgtcggggc tggacgagtt
      541 caagtccatc tccttgctga agccgtccgc gtagatgagg aaggtgcgcg cccaccccac
      601 agggagcgcg ggaagctgtg cggcgtcgaa ggagatggcc gcctcgtcgc cggggcgcgc
      661 gacgacgaac atgtcatcga cgccgccgag gagcagcggc agcacgtcgc cctcgcgcgt
      721 gtagcggccc ggcatcacct tccacggcga cgtgtgcgag acgcgcgcgt agtcgtaccc
      781 gtatggctcg cgcccgtccg gcgtctcctc ggccgagaag ccgcgccagc gcagctcggc
      841 cgacgcgggt tcgagcgtcg tcatcttcac ggggaagtcg ccgccggagg tgtccaacag
      901 aacctgatcc cagtagacgc gcatgtttgt gacgacgcgc acctcgcgcg cgccggcagg
      961 aagtttgccc gtcagatcga cgacgaccgt ctgcgggcgg cctacgggca cgccgatgtc
     1021 ctcgatgacg gtctgccagc gcccgcccgc gtctttgact tgcagcgcgg gcggtcgcag
     1081 cttcataccg ctctgcgcgg cggcgacgtt gtcgctcgaa aaggcgtagt ccgtccagcc
     1141 cgtcaacagc agcagcgtgc gcccacgtaa gggtgagggc ggagggatga gggatgaata
     1201 agagcttcca tgttttacat catccctcat ccctctgccc tcatccctcc tttcatccct
     1261 gccgaggtcg agcgtcagcg tgtgttcctc ggcgtagccg cgtatccggt ggagcgcgaa
     1321 gtcgtcgggg tagcgacgat ccatgtgcgc gatgcgcgcg agcacgtcgt ggccgtgctc
     1381 gtcgcgggcg gcgaagggcg cgcgcgacga gcgcgtggcg tagagcttga gggcggggcc
     1441 atcgggacgg ccgagtcctt cgttcggata gacttcgacg cccgccggat gatcgacggc
     1501 gacgagtcgg aggcggtcga ggaagagcac ttcttcgagt tcgttggtga cgcgaatctc
     1561 gtagcgcccg ccgcgggcct tgagttggtc gccgcgcacg cgcacgtatt cgtccgtgtc
     1621 gggcgcggag cgcacgcccg gcgccagcca gtagcccaac tcgccgccgc cgaggaagtc
     1681 cgtgatgaac tcgaagcgct cgccgttcca agtgtaaagg tacgggcaag acgacggctt
     1741 gcggtcgagt tccgtgacgc tcacgctcga cactgcgccc acgcgcccgc gccccacagc
     1801 ggcggcgccc gtcgcggaaa tctccgtctc ggcctgcacg atgccggccg gccacagcac
     1861 gcgcacggcg tcggcgcgtg cgcgcccgcc cagtccgaac accacgtcgg ccggagccgg
     1921 agccggcatg gccgaatagg tttcgtgctt ctggctgaga cttcccgcgc gcatctccac
     1981 cttcgcgccg acgccgctgc ggttgctcac cttccccgcg aggcgcacgc gcacggcgcc
     2041 gcggcggttg ccgccttcgt tgcgcgcgaa gacaaggggg ccgccctcgc gcctgaagac
     2101 gaggtcggtg tcgccgtcag cgtccacgtc gccggagacg aggacgcgcg gcgacggtct
     2161 cgcgtccgaa gctgcgctga acaagccacg cgtggcgtgc tcgctcacgt cggcccaacc
     2221 gcgccccgtg ttgcgccaga cgcccacctc gcctgatgtg tcgttgatgg cgacagcgac
     2281 gaggtcgagc aggccgtcgt tgtcgtagtc gaggaactgc gccgcgtggc gctccacgga
     2341 tttcgcgctc atcccgctct cggcgcgccc tgctggcccg tccacgacgt tgaaacggcc
     2401 tttgccgtcg ctcatggcga agtagccggg gaaggcgccg cggccgaagt aaaagtccgt
     2461 aaagccgtcc ttgttcacgt cgccggcggc ggcgcaggtc agccccgtag cgtcgaagcg
     2521 caggccggct tccgtcccca cgtcgcggaa cgtgccgtcg cgcaggttgc ggtagagcgc
     2581 gggctgtttg tcgtgataga cgacgagcag gtcaacgtcg cgccggttgt cgtagtcggt
     2641 cggcacgacg gcgaccgcgt ttcccttcgc ggagagaccg gcctccgcgg tgaagtcttt
     2701 aaaggcgccg tcgccgttgt tgcgcagcag gaggttcggc gcgtcaacgg gcgtgcgacg
     2761 cgcttcgccc ggctgactct cgcgggccgc gtcagtcgcg ctttgcgcga agccggcgac
     2821 gaagatgtcg aggtcgccgt cgtggtcggc gtcgaggaag gccgcggaga tcgagaggtg
     2881 cgggtaggcg ggaatcccgg ccgcggcggt cacgtcggcg aagagaccgc gcccctcgtt
     2941 gcggtagaga ttgagcgcgc cgttgcgcag cacgaagagg tcggggcgct cgtcgttgtt
     3001 gaagtcgccg gccagcgcac cgatgccgat gcccggcgcg gcttcagcga gcgcgcccga
     3061 gcgagacgtg acatcgacga gccggccgcc gtcgttgcgt cggagtcgct gcgacgtggg
     3121 tgtgacctcg aagaggtcga ggtcgccgtc ggagtcgaag tcgaagagcg tcacgccgcc
     3181 gccgagcgcc gcggcgagcg aacgcttccg ctctcccgcg cgcggcgaag tggcgtcggg
     3241 cttggccgcc gccctgcgcg cgcgcctgct gccggccgcc cttccctgtg gcatcacact
     3301 cgccgtcgcg tcggtgaagg cgacgcgcgg agttgtgggg tcaactaagt ctggctcggc
     3361 gccggtcgag gcgacggcct ccgcgtagcg gccctgttcc agatagttcg ggccgatgac
     3421 ggtcgcggcg ccgctctgac gaagcgcctg aaaacgctcg atcatgcgct gcccctcttc
     3481 gcgctgcccg gcacgcagga gcgcggtgcc gaggttgtag agcgccgtgc cgttgtaagg
     3541 ctcctccgag agtgcggcgc ggaaggcttc gacggcctcg gggtacttgc gctgctgtgc
     3601 gaggagctga cccgtgttga cgagtgcgcc cacgtcgcgc gcgtccgcgt cgaggacgcg
     3661 gcggaaggag gcgagcgcgt cgtcggggcg gttctgcgag cgcgcgatga gaccgaggat
     3721 gtagtgtggc tgcggcgcgc ggggcgcgag cgcgacggcg gcctcggcct cgcggcgcgc
     3781 ggcgtcggcg tcgggcacgt tgaagagcgc gacggcgagg ttgatgcgcg cgagcgcaag
     3841 cccagggtcg agccggagcg cgcggcggaa ttgttcggcc ccgtcggcgt gccggaactg
     3901 ttcgagcagc gcgacgccga gattgttcgc gcgatacgcc tcctcgcgcg tgtttgtcgg
     3961 gccggtctgt tccccgccgc gagcgcgtct ttgcgtgccg gcgcggggcg gcgcggcgag
     4021 cggaatgaag accagacaga ggacgaggga ggcgaattgg cgcgtgcgta ttttcatggt
     4081 taactgtcgg ccagtgtgtt ttcgtatggg ctggagagag tagacactga cgcggcgcgc
     4141 gagcgcaagt ttgtgcgcgt cgttgctcac agggggcgtc gtcgtcgggc agggcaactg
     4201 gcagcagcag ttggagcaaa gcggcagcgc cggcagcccc gttacgaacg cgcaacgggg
     4261 ctgccggctt ttgtttcaaa atccaagctt tatttgttgt gataaggacg cagcgacaac
     4321 cgcgacgagc gcttggcctg agattgcgac agagaagatg attatcattg acggctcatt
     4381 cggcgagggc ggcgggcaga tcatccgcac ctcgctcgcg ctctcgctca tcactggaaa
     4441 acctttccgc gtgatgaacg tgcgcgcgcg gcgcgaccgt cccggcctcc agcgccaaca
     4501 cctgacggcc gttaacgccg ccgccacggt cggggccgcg cgcgttgaag gcgcggccat
     4561 cggcgcgcgc gagttcacgt tcgtccccgg cgaggtcacg gctggcgagt actcgtttcc
     4621 cacggggacg gccggcagca ccacgctcgt cgcgcaggcg attctgccgc cgctcatgct
     4681 ggccgccgcg ccgagcgtcg tcacactcga aggcggcacg cacaacgtcc acgcgccgcc
     4741 cttcgagttt ctggagaaga ccttcgtgcc gctcgtcaac cggacgggcg cgggcgtcag
     4801 tgtcgaactc gtgcgctacg gcttctaccc gccgggcggc ggccggcttg acgtgctcat
     4861 cgagccgcgc gccgtcgcgc gccgcctcga catcacggaa ctcggcggcg tgcggcgtgc
     4921 cggcgcacgc gcgctcgtcg tcaaactcgc gccctcggtc ggcgagcgcg cactggaagt
     4981 ggtcaggcga aaacttggct gggagggcga cgcgctccgg ctcgaactct cacaggacgc
     5041 gctcagcccg aacgtcgtcc tgacgctgga ggtcgagagc gagcacctga cggaagtctt
     5101 cacgggggtg ggcgagcgcg gcgtgcgcgt cgagacgatt gccgagcgcg tgtgcgacga
     5161 ggccgcgcgc tatctggaga tcggcgcccc cgtcggcgcg cacctcgccg accaactgct
     5221 tatccctctg gcgctctcgg gcggcggctc atacatcacg gggccgctct cacttcacac
     5281 gacaaccaat attgaaatcg tgaagaagtt tctgcccgtc gaaatcacgg cctcgcactt
     5341 gagtaatgag ttgtggaagg tggaggtctt tgcctgaaca agagtgtgtt ccgcgttgga
     5401 ctcaccgccg agtcgcaaag gacgcagagt tcacgcgaga gggttgagtc tattcaagag
     5461 acgtttcaaa tgacacacga ccggcccatc aagctctacg tcttcgacgc cgacggcacg
     5521 ctccgccgca cgactgttga aggacaaccg tgcccgaaca agcccggcga gtgggagttg
     5581 ataccgggcg tgcgcgagcg cctttcgcaa atcgactgga cgtgcgcgag ctttggcgtc
     5641 gcctccaatc agggcggcgt cgggacgggc tacatgagac atgaggccgc gtggcaattg
     5701 ctcttcgaca tggccgccgc ggccctcggc tccgagccgc cggccggctc aatcgagatg
     5761 tgcccgcacg cgccatactc gaactgcccc tgtcgcaagc cgaagccgct gatgctccgc
     5821 cgcctgatgc accgcttccg cgcgacgaag agggagacgc tcttcgtcgg cgacatggat
     5881 cgcgacaagg aggcggcgcg gcgcgcgggc gtccgctttc tgtgggcgca cgagttcttc
     5941 gggtgggaga cgccgggggc ttagacctct tccggccccc agatgtccac gtcgagttct
     6001 tcggcgtagt ggagcagggg ttcgcccgcg ggagtgggca ggccgtgcga ctcgatcatg
     6061 gtggagtcga gcgtgaggag ttcggcgtcg cggagcggcc acggctcgtg gtggatgcgc
     6121 gcgcgcatca atcggtcgcc gcgcagggcg tagaggcagt agcgttcgac gaggaagaac
     6181 tccagcgtgt cggggcgggc gaagcgggag ttgccgcccg ttttccagga ggcgtgaaac
     6241 tcggcgggcg gatcgtccgt gcgcgcgagg tcgtagttga tcgtctcgcc ctcctgttcg
     6301 agcgcgatct cggcgtggaa gtagggtagt tggtagaagg tgcgcgcgct cacgacggcc
     6361 gctcggctgt tggcgtcgag cgagaagaac cagacgccgg gcacgccgtc cacgtggacg
     6421 taggttctga cgttcaactc atggagcgag ctgaggccgg gcacgggcgg cgcgaaactc
     6481 agccgcacgt tccacatcgt aaacggcgtg acggcgatcc acgcgctgcc gtcgaaggtg
     6541 tcgatggaaa gcgactcggg gatgaggggg cgcaacactt cccggtcgat gcgccagtgc
     6601 atgaagagga gcttgcccca ggtctggcgc atgaggggca tgccctcggg ccgcgtgcgc
     6661 aaggcgaggc ggtcgagggt ttcggggcgc gggtctttca tattcgacat tcaaattgag
     6721 cgattagcga ttagctgtta gctttcagcc agagaccggc gtcgtcgccg ctgacactca
     6781 ataacttggt tcgctctctc atagctaaca gctaacagcc aatcgctaac agctccttag
     6841 tgtaatgtct tgccgtcgcc gcggccaacg tcttgcttgc cgaccgtggg cttgccctgc
     6901 tcggcgggtg tgcgcttgcc gcccttgcgc gggcggtcgt tgcgcgccgc gctcggcggc
     6961 gacttgcccg cgcgcgactc gccggggtcg gcctgccgtt tactcttcgc cattcgtgtc
     7021 gtcctccctt cggtcgttgc tgccgaaaaa ggtctcgctt catccggccg tcgtgtcgcc
     7081 gccgagcagg gtgagcacct cgccggtaat gtaactcgaa tcggcctcgg aggcgaagaa
     7141 gacgaaggcc ggcgcaatct cctcgggctg cgcgggccgc ttcatcggcg tgtcggcccc
     7201 gaactcggcg acctctcggg ccggcttgtc cgaa
//
LOCUS       DASVES010000332        10239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1025965, whole genome shotgun sequence.
ACCESSION   DASVES010000332 DASVES010000000
VERSION     DASVES010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025965"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1668
                     /locus_tag="VF240_13635"
     CDS             10..1668
                     /locus_tag="VF240_13635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX7176299.1"
                     /translation="MSNVLEIIPLGGLGEFGTNCLCLRYGDEMIVVDVGMGFPEETAY
                     GVDVSVPDFDFLEEYRDSLLAIILTHGHEDHLGALPYLLKKHNLPVYCSHFTAGLAES
                     KLHEHDLTDSVLLHRVDPRDVVEIGSFSVEFIRVSHSLVDCFALAITTPVGTLIHTGD
                     YKVDETPVIGEPIDLRTLRRYGQEGVLALTSDSTNATVPGRTPSERAVIPAFEEIFNE
                     AEGRIIVAAFSSSIHRLQIVLDVAQQFDRKVCVLGRSMLKNVEIADRLGFLDLHDGML
                     VNLNEAKRLADDEIVFLVTGSQGEARAALSQIATQSYKGLQIREGDTVVLSARIIPGN
                     ERAISRLIGEIYKRGGKIIDEKRRLVHVSGHASQEDIRILTEAVRPKFVIPVHGEYRM
                     LYRHKEFLRNHMDFRDDQVILIENGDVLELDGESATVVGKREVGRTFIDDSGFEEIDG
                     ETVRERRQLAYDGVITPVVTISEETGELELPPEIVTRGVIGIDGGGLVRDMQKVVTKT
                     VEGASKEELRDTSLLKERVRLDLKRYVQKQTGARPVIVPVVVQI"
     gene            1743..2186
                     /locus_tag="VF240_13640"
     CDS             1743..2186
                     /locus_tag="VF240_13640"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7176300.1"
                     /translation="MSATDAVELLRRTRVEVAPQTFVVVGMRHEDWGRLLESPELSPR
                     ADSHFMLLRDRFEVTLVVEQEDWRAMRHAARDARVEGDFRLLTFDIELPWHVVGFLAR
                     VTEILAVEGVSVGALTAFSRDHLLIRQEDLGRALRALGPHVEELC"
     gene            2200..2691
                     /locus_tag="VF240_13645"
     CDS             2200..2691
                     /locus_tag="VF240_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176301.1"
                     /translation="MDLLSIIGSLASVATAIGVFVAGWQIRLAKQLARTQFEDTLTQQ
                     FREIIWKIPVDALIGREISEEQYQQTRDDFFRYIDLSNEQVFLRKNNRISKATWELWR
                     DGMKAFLARPSFERAWKEFKAASPDIFKELRRLEDEEFDTDPCTWVQQAVVASKGGVL
                     IEH"
     gene            2681..3451
                     /locus_tag="VF240_13650"
     CDS             2681..3451
                     /locus_tag="VF240_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7176302.1"
                     /translation="MNIDWKAKVVLITGASSGIGRALAVELGRRGASVALLARRAEEL
                     LKVAEEVEREGGRALTLPADVRVATEVSAAVARVEELWGRVDVLVANAGFATVKAASE
                     LRAGEVSDMIAVNFIGASNSVSAVMPGMIKRGSGHLVAISSLASFRGLPKSAAYSASK
                     AAMSTFFESLRVDLKRSGIMVTTIHPGFIRTPLTDGRKSLPFLVEVDEAARKIVRAIE
                     LRRRTYAFPWQLAGLVGLARFLPDALYDRIASRKNFRE"
     gene            3592..4494
                     /locus_tag="VF240_13655"
     CDS             3592..4494
                     /locus_tag="VF240_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7176303.1"
                     /translation="MTYLQAIILGIVQGLTEFIPISSTAHLVFAARLTQLYEGEAMRA
                     EMTTATIAVIQLGTLLAVFVYFAGDIWAISNAFVRDHLALLRGRRGAGVGGGLRGALA
                     GLSEEARLGWLIVIGSIPVGVVGLLLKKQIEGPWTKNLWLIATMMVVVALMLLVAEKV
                     GARRKEMKDLGVGDALAVGFAQVLSLMPGASRSGSTIMGGLFAGETRETAARFSFLLS
                     IPAIAASGLLELGEAIEKLPASSAAPLVVATVISGLVGYASIWFLLRFLRTRSTGVFI
                     IYRIVVGLAIMLLLARGILQPFDS"
     gene            4693..5016
                     /locus_tag="VF240_13660"
     CDS             4693..5016
                     /locus_tag="VF240_13660"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7176304.1"
                     /translation="MDDPKQERISPAQRPRSPRVPVNFAVELEGRDAAGEPFHVQAEA
                     IRVSRGGATLISEVRLQVGAAVRLTPPFGRTLDAEVNGVWTEESDGRQHIGVKLLNEN
                     GWFAE"
     gene            5018..6769
                     /gene="recJ"
                     /locus_tag="VF240_13665"
     CDS             5018..6769
                     /gene="recJ"
                     /locus_tag="VF240_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX7176305.1"
                     /translation="MAIATTGTKATARKRWEVRADDERAAGELARRLSLSPIVASVLV
                     ARGVKSEEEARALLNPSAEQLHDPSLMLGMTESVARVLRAVDTGEPVLIYGDYDVDGT
                     TGTVVLRRALQVLGAKTGFHIPHRFTEGYGIRQDTLERAKAEGYGLVVSVDCGIRAHE
                     PLEWARANGLDVIVTDHHLPDDDEGSPPAFAVLNPNQRGCPYPDKNLAGVGVAFKLAH
                     ALFRERGREHLVKGFLKVVAIGTVADVAQLVGENRVIVALGLADIHKATNPGLRALLE
                     IAGCGDGRGMRASDLGFRLGPRINAAGRMDAARAVVELFETTDHEEARRLAEHLDARN
                     RERQTMQRLITERAVVELETGGFADAPVAIVAGDGWHRGVIGLAASKICERLGRPTVV
                     ISFDEDGHGHGSARSPDGYHLLDGLTTCADLFDGFGGHAHAAGLAIRRENVPELRRRL
                     SEHAARAATAAGPAGIAPAVEIDAELSPESLSLELYEELSALEPYGAGWPRPVFLTRD
                     LRVVGEPRVLKERHLKFAVAGDDGRVHSCIWWGAAESITATPRTGERIELAYTLESNT
                     WREETRLQLCVEDMRSK"
     gene            6867..9278
                     /gene="lptC"
                     /locus_tag="VF240_13670"
     CDS             6867..9278
                     /gene="lptC"
                     /locus_tag="VF240_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX7176306.1"
                     /translation="MQEITRSRATWIGARARAPLVARVLALLALFGGIAFIGVSVYRS
                     RGVEKFVMRGGAAELSKDVVRVVEGYEHREMKDNRLHILLRASREITYTDGHHELEDV
                     SLEVYPETGDRPDRVTAKRTIITDQNMRVLFTGDVNVETRDALKAKTESINYDIRNEV
                     GVSDVPLTFERENVHGRADAATVDAKNKKLDLRGNVEIVVEPKAKDAVAKPGARERPI
                     TIRSPLANFDQNALTLVFSGGATAEQEQDVFSGDILTGFLNAAKKIERIEARGNSYLR
                     SLSDGHAAEVHSADMDFLFDPDQKIKYAKASRDARGRSLDADAEVAFTTPGDAFVDFT
                     PQGERSLISRLVINNRPVITLAAPKSKAGDPNAANKRLISDSVRLFWRATGKDLERAE
                     AEGNAELYVEPVTPSPTADRKTLTAPRFDGDFYEAGNLAKTFVAQGGAKAVIDPIQPS
                     ETRATRTLTSARMTANFVRETQDVERIDADGDARFVERERTLTSQKMEAVFGQGQAMQ
                     KVEAKVNAKFNERDRNGEAATMTYTAGDEVVRLRGGEPVVWDARARLKAPEIDSDMRN
                     KISYARGRVATTYYSQEQTGGAAPFKKTKSPIFIASQDAEFQHETGVGIYTGAARAWQ
                     DDNFVKADRLTLRREQKRMEGDGNVQSALYQARRKDAAGNRSVVPVFATSARMFYADP
                     ERLLHYETSVDIKQGTERIMANAADVYLQPETYEAERTVAQGNVVVTQPGRRGTGDWA
                     QYTAADESVVLTGNPARVEDAAKGDSASNRMTVYLREDRIVSDAGESKQSTGRVRSSH
                     KIKKQ"
     gene            9361..10164
                     /gene="lptB"
                     /locus_tag="VF240_13675"
     CDS             9361..10164
                     /gene="lptB"
                     /locus_tag="VF240_13675"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX7176307.1"
                     /translation="MQTEEIIETRSGEAPAERSAPDVGVPSPTALAAFGLVKSYGKRR
                     VVDEVSLHVEQSEVVGLLGANGAGKTTTFYMMVGLEQPNAGRVMLGKREITKLPMYLR
                     ARLGVGYLPQEPSIFRKLSVEENLLAVLEHMPMRRREQLKRVEELLEEFGIAQVRRTR
                     GDRLSGGERRRTEIARAIATDPQYILLDEPFAGIDPKAVDELQNLILYLSSRGIGILI
                     TDHNVRETLGVTNRAYIMAEGKIFRAGAPLELTDDPEVRRLYLGEKFRM"
BASE COUNT         1886 a         3419 c         3242 g         1692 t
ORIGIN      
        1 aactaaacag tgagcaacgt ccttgagatc atcccgctcg gcggcctcgg cgagtttggg
       61 accaattgtt tgtgtctgcg ttacggcgac gagatgatcg tcgtcgatgt cgggatggga
      121 ttccccgagg agaccgccta cggcgtggac gtttccgtcc ccgactttga cttcctcgaa
      181 gaatatcgcg actccctttt ggccatcatc ctgacgcacg ggcacgagga tcacctcggc
      241 gcgctgccgt acctgttgaa gaagcacaac ctgccggtct actgctccca cttcaccgca
      301 ggactggccg agagcaagct ccacgagcac gacctgacgg actccgtgct gctccaccgc
      361 gtagacccgc gcgacgtggt cgagatcggc tccttcagcg tcgagttcat ccgcgtctcc
      421 cattcgctcg ttgactgctt cgcgctcgcc atcaccacgc ccgtcggcac gctcatccac
      481 accggcgact acaaggtgga cgagacgccc gtcatcggcg aacccatcga cctgcgaacg
      541 cttcggcgct acgggcagga gggcgtgctc gcgctcacct ccgattcgac gaacgccacc
      601 gtgccgggcc gcactccctc ggagcgcgcc gtcattccgg ccttcgagga aattttcaac
      661 gaggccgagg ggcgcatcat cgtcgccgcc ttctcgtcgt ccatccaccg gctccagatc
      721 gttctggacg tggcgcagca gttcgaccgc aaggtctgcg tcctcggccg ctcgatgctc
      781 aagaacgtcg agatcgccga ccgcctcggc ttcctcgacc tgcacgacgg gatgctcgtc
      841 aacctcaacg aggccaagcg gctcgccgac gacgagatcg tcttcctcgt cacggggtcg
      901 cagggcgaag cgcgcgcggc cctgtcgcag atcgccacgc agtcctacaa gggattacag
      961 atcagagaag gcgacacggt cgtgctctcg gcgcgcatca tccccggaaa cgagcgcgcc
     1021 atctcgcgcc tcatcgggga gatttacaaa cgcggcggca agatcatcga cgagaagcgc
     1081 cggctcgtcc acgtctcggg ccacgcctcg caggaggaca tcaggattct gaccgaggcc
     1141 gtccggccga agttcgtcat ccccgtccac ggcgagtacc gcatgcttta ccgtcacaag
     1201 gaattccttc gcaaccacat ggacttccgc gacgatcagg tgatcctcat cgagaacggc
     1261 gacgtgctcg aactcgacgg cgagagcgcg accgtcgtcg gcaagcgcga ggtcggccgc
     1321 accttcatcg acgattccgg cttcgaggag atcgacggcg agacggtgcg cgagcgccgg
     1381 cagctcgcct acgacggcgt catcacgccc gtcgtcacga tcagcgagga gacgggcgaa
     1441 ctcgaattgc cgcccgagat cgtcacgcgc ggcgtgatcg gcatcgacgg cggagggctg
     1501 gtgcgcgaca tgcaaaaggt cgtcaccaag acggtcgaag gcgcctcgaa ggaggaactg
     1561 cgcgacacgt cgctgctcaa ggagcgcgtc cgcctcgacc tcaaacgcta cgtgcagaaa
     1621 cagaccggcg cgcgccccgt tatcgtcccg gtcgtcgtgc aaatctgaga atgaagacag
     1681 cgaggcgacc gccccccggc cgtcgtctcg tctcgtcagg caccttcgat aaacagcctc
     1741 atatgtctgc gacagacgcc gtcgaactgc tgcgccgcac gagagttgaa gtcgcgccgc
     1801 agactttcgt cgtcgtcggc atgcgccacg aggactgggg gcggctgctg gaaagccccg
     1861 agctgagtcc gcgcgccgac tcgcatttca tgctgttgcg cgaccgcttc gaggtgacgc
     1921 tcgtcgtcga gcaggaagac tggcgggcca tgcgccacgc cgcgcgcgac gcacgcgtcg
     1981 agggagactt ccggctcctg acgttcgaca tcgagctgcc gtggcacgtc gtcggcttcc
     2041 tcgcgcgggt gacggaaata ctcgctgtcg aaggcgtctc ggtcggcgcg ctcaccgcct
     2101 tctcgcgcga ccatctgctc atcagacagg aagatttggg ccgggcgcta cgtgccctcg
     2161 gcccgcacgt ggaagaactc tgttgacgga gtcgggcgca tggatttgct gagcatcatc
     2221 gggtcattgg cctcggtggc gacggccatc ggcgtgttcg tcgcggggtg gcaaattcgc
     2281 ctcgccaagc aactcgcccg gactcagttc gaggacacgt tgacgcagca gtttcgcgag
     2341 atcatctgga agattccggt tgacgcgctc atcggacggg aaatcagcga ggagcagtac
     2401 cagcagacgc gcgacgactt cttccgctac attgacctgt cgaacgagca ggtgttcctg
     2461 cgtaagaaca atcgcattag caaggcaacg tgggagcttt ggcgcgacgg gatgaaagcc
     2521 ttcctcgcac gcccgtcgtt cgagcgggcg tggaaagaat tcaaggccgc gtcgccagac
     2581 atcttcaaag agcttcgtag actggaagat gaggaatttg ataccgaccc atgtacctgg
     2641 gttcaacagg cggtagtcgc atcaaaaggt ggcgtgttaa ttgaacattg attggaaagc
     2701 aaaagtcgtt ttaatcacgg gcgcgtcgag cggcatcggg cgcgcgttgg ccgtggagtt
     2761 ggggcggcgc ggcgcgtcgg tcgccctttt ggcgcggcgg gccgaggagt tgttgaaggt
     2821 cgccgaggag gtggagcgcg agggcgggcg ggcgctcaca ctgccggcgg acgtgcgcgt
     2881 cgcgacggag gtgtcggcgg cggtcgcgcg cgtcgaggag ctatggggtc gggttgacgt
     2941 gctcgtggcg aacgccgggt ttgcgacggt caaggccgcg tcggagctgc gtgccgggga
     3001 agtgtcggac atgatcgccg tcaacttcat cggcgcgtcg aacagcgtgt cggccgtgat
     3061 gccggggatg atcaagcggg ggagcgggca cctcgtcgcg atctccagcc tcgcgtcgtt
     3121 tcgggggctg ccgaagtcgg ctgcctacag cgcgagcaag gcggcgatgt ccaccttctt
     3181 cgagtcgctg cgcgttgacc tcaagcggag cggcatcatg gtgacgacga tccaccccgg
     3241 cttcattcgc acgccgctga ctgatgggcg caagagtctg ccgttcctcg tcgaggttga
     3301 cgaggccgcc cgtaaaatcg tccgcgccat tgaactgcga cgccgaactt acgccttccc
     3361 atggcagctc gccggcctcg tcggccttgc ccgattcctg cccgacgcgc tctacgaccg
     3421 catcgcctcc aggaaaaatt ttagagaatg atgaaccagc tattagctgt tagctgttag
     3481 ccttcagcca atcgagattt tttgtgttcg ccgtgacggc gttcgcgttg atgcgtctat
     3541 cgacactgac ttgctagcta acagctaatc gctaatcgct aaaagctccc tatgacctac
     3601 cttcaagcca tcatcctcgg gatcgtgcag gggttgaccg aatttatccc catcagctcg
     3661 acggcgcacc tcgttttcgc cgcgcgtctg acgcagctct acgagggcga ggcgatgcga
     3721 gccgagatga cgaccgccac catcgccgtt atccaactgg gcacgctcct cgccgtcttc
     3781 gtctacttcg ccggtgacat ctgggccatc tccaacgcgt tcgtgcgtga ccatctggcg
     3841 ctgctacgcg gccggcgcgg cgcgggcgtc gggggtggac tacgcggcgc actcgcgggt
     3901 ttgtcggaag aggcgaggct tggctggttg atagtcatcg ggtcgatacc cgtcggcgtg
     3961 gtgggcctcc tgttgaaaaa gcagatcgaa gggccgtgga cgaagaacct ctggctcatc
     4021 gcgacgatga tggtcgtcgt cgcgctcatg ctcctcgtgg ccgagaaggt tggcgcgcgg
     4081 cggaaggaga tgaaagacct cggcgtcggc gacgcgctcg ccgtcggctt cgcgcaggtg
     4141 ctctcactca tgcccggcgc ctcgcgctcc ggctcgacca tcatgggcgg cctcttcgcg
     4201 ggcgagacgc gcgagacggc cgcgcgcttc tcattcctgc tctcgatccc ggccatcgcg
     4261 gccagcgggc tgctcgaact gggggaggcc atcgaaaaac tgccggcttc aagcgccgcc
     4321 ccgctcgtcg tcgcgaccgt catctcgggc ctcgtcggct acgcctcgat ctggttcctc
     4381 ctgcgcttcc tgcggacgcg ttccaccggc gtcttcatca tctaccgcat cgtcgtcggc
     4441 ctcgccatca tgctcctgct cgcgcgcggc attctccagc cattcgacag ctagggcaat
     4501 tcaagtaaaa agtaaaaagg caaaaggcaa aagtgaggga gcaaggtcgc ctcttcactt
     4561 ttgccttttg cctttttact tttgccttcc tctacgctcg cagtcgcgcc cggcatcggt
     4621 taaacttgag tcggcccctt actcacgagc gatcaaaatc ctgtagcaag gaagagggct
     4681 gtgagttcac acatggacga tccgaagcaa gaaagaattt cccccgcgca gaggccccgc
     4741 agcccgcgcg tgccggtcaa tttcgccgtc gagcttgaag ggcgcgacgc ggcgggtgag
     4801 ccgtttcacg tgcaggccga ggccattcgc gtcagccgcg gcggcgccac gctcatctcc
     4861 gaagtccgcc ttcaggtcgg cgcggccgtc cgcctgacgc cgcccttcgg acgcacgctc
     4921 gacgccgagg tcaacggcgt ctggaccgaa gaatcggacg ggcgacagca catcggcgtc
     4981 aaacttctta acgaaaacgg ctggttcgcc gagtaggatg gcaatagcga cgacgggcac
     5041 gaaagcgacg gcgcgaaagc gttgggaggt gcgcgctgac gatgagcgcg cggccggaga
     5101 actcgcgcgc cgcctcagcc tctccccaat cgtcgcctcc gtcctcgtcg cccgtggcgt
     5161 caagtcggaa gaggaggcgc gagcgctcct caacccttcc gccgagcaac tacacgatcc
     5221 ctcgctcatg ctcgggatga ccgagtcggt ggcgcgtgtc ctgcgggccg tggacacggg
     5281 cgagccggtg ctcatctacg gcgactacga cgtggacggc acgaccggca ccgtcgtcct
     5341 gcgacgcgcg cttcaggtgc tcggcgcgaa gaccggcttc cacatcccgc accgcttcac
     5401 cgaaggctac ggcatccggc aggacacgct ggaacgcgcg aaggccgaag gctacggtct
     5461 ggtcgtcagc gtcgattgcg gcatccgcgc ccacgagccg ctggagtggg cgcgcgctaa
     5521 cggcctcgac gtgatcgtga ccgaccacca cctgccggac gacgacgaag ggtcgccgcc
     5581 cgccttcgcc gttctcaatc caaatcagcg cggctgcccg tatcccgaca agaacctcgc
     5641 cggtgtgggc gtcgccttca agctcgcgca cgcactcttc cgcgaacgcg ggcgcgaaca
     5701 cctcgtcaaa ggatttctga aggtcgtggc catcggcact gtagccgacg tggcgcagct
     5761 cgtcggcgag aatcgcgtca tcgtcgcgct cggcctcgcc gacatccaca aggcgacgaa
     5821 tccgggcttg cgcgcgctcc tcgaaattgc cggctgcggc gacgggcgcg ggatgcgcgc
     5881 ctccgacctc ggcttccgac tcggcccgcg catcaacgcc gccggccgca tggacgccgc
     5941 ccgcgccgtc gtcgaactgt tcgagacgac cgaccacgaa gaggctcgcc gcctcgccga
     6001 acacctcgac gcgcgcaacc gcgagcgcca gaccatgcag cgcctcatca ccgagcgcgc
     6061 cgtcgtcgaa ctcgaaacgg gcggattcgc cgacgcgccc gtcgccatcg tcgccggcga
     6121 cggctggcac cgcggcgtca tcggcctcgc cgcctccaaa atctgcgagc gtctgggacg
     6181 cccgaccgtc gtcatctcgt tcgacgaaga cggccacggc cacggctccg cgcgcagccc
     6241 cgacggctac cacctgctcg acggcctgac tacctgcgcc gacctcttcg acggcttcgg
     6301 cggccacgcg cacgccgccg gcctcgccat ccgccgcgag aacgtccccg agctgcgccg
     6361 ccgcctctct gagcacgccg cgcgcgcggc cacggcagcc ggccccgcag gcattgcccc
     6421 ggccgtcgag atcgacgccg agctttcgcc cgaatcgctc tcgctcgaac tctacgaaga
     6481 actgagcgcc cttgagccat acggggcggg ctggccccgc ccggtgtttc tgacgcggga
     6541 cttgcgcgtg gtcggcgagc cgcgcgtctt gaaggagcgt cacctgaagt ttgccgtcgc
     6601 gggcgacgac ggccgcgtcc acagttgcat ctggtggggc gccgccgagt caattacagc
     6661 aacgccccgc acgggcgagc gcatcgaact cgcgtacacg ctcgaatcaa acacctggcg
     6721 cgaagaaacg cgcttacagt tgtgtgtcga agacatgaga agcaagtgat aagtgatgag
     6781 tgataagtga taagaaagag ataagcggat tttcttatca cttatcactc atcacttatc
     6841 actcatcact tatcacggtt ttaaagatgc aggagatcac ccgcagtaga gccacgtgga
     6901 tcggcgcgcg cgcccgcgcg ccgctggtgg cgcgcgtgtt ggccctgctg gcgctgttcg
     6961 gcgggattgc tttcatcggg gtttcggtct atcgctcgcg gggcgtcgag aagtttgtca
     7021 tgcggggcgg cgcggcggag ttgtcgaagg acgtggtgcg cgtcgtcgaa ggctacgagc
     7081 accgggagat gaaggacaac cgcctgcaca tactgctgcg cgcctcccgc gagatcacct
     7141 acacggacgg ccaccacgag cttgaggatg tgagcctcga ggtctacccc gaaacgggcg
     7201 accgccccga ccgcgtcacc gccaagcgca ccatcatcac cgaccagaac atgcgcgtcc
     7261 tcttcaccgg cgacgtgaac gtcgagacgc gcgacgccct caaggccaaa accgagtcga
     7321 tcaactacga catccgcaac gaggtcggcg tctcggacgt gcccctgacc ttcgagcgcg
     7381 aaaacgtcca cgggcgagcc gacgccgcga ccgtggatgc taaaaacaag aagctcgact
     7441 tacgcggcaa tgtcgagatc gttgtcgagc cgaaggcgaa ggacgcggtg gcgaagcccg
     7501 gcgcgcgcga gcggcccatc accattcgtt cgccgctggc caatttcgac cagaacgcgt
     7561 tgacgctcgt cttctccggc ggggccacag ccgagcagga gcaggatgtc tttagcggcg
     7621 acatcctcac cggctttctc aatgccgcaa agaaaatcga gcgcatcgag gcgcgcggca
     7681 actcctatct gcgctcgctc tccgacggcc acgcggccga ggttcactcc gccgacatgg
     7741 acttcctctt cgaccccgac cagaagatca agtatgcgaa ggcgagccgc gacgcgcgcg
     7801 gccgcagtct cgacgccgac gccgaggtcg cctttacgac accgggcgac gccttcgtgg
     7861 atttcacccc gcagggcgag cgcagcctca tctcgcgcct cgtcattaac aatcgccccg
     7921 tcatcacgct cgcggcgccg aagtccaaag cgggcgaccc caatgcggcc aacaagcgcc
     7981 tgatctctga ctcggttcgc ctcttctggc gcgccacagg caaagacctc gaacgcgccg
     8041 aggccgaggg caacgccgag ctgtacgtcg agccggtgac gccctctccc acggccgacc
     8101 gcaagacgct caccgcgcca cgcttcgacg gagacttcta cgaggccggc aacctcgcca
     8161 aaaccttcgt cgcgcagggc ggcgcgaagg ccgtcatcga tcccatccag ccctccgaga
     8221 cccgcgccac gcgcacgctc acctccgcgc gcatgaccgc caatttcgtc cgcgagacgc
     8281 aggacgtgga gcgcatcgac gccgacggcg acgcgcgctt cgtcgagcgc gagcgcacgc
     8341 ttacctcgca gaagatggag gccgtcttcg ggcagggaca ggccatgcag aaggtcgagg
     8401 cgaaggtaaa cgccaagttc aacgagcgcg accgcaacgg cgaggcggcg accatgacct
     8461 acacggcggg cgacgaagtg gtgcgcctgc gcggcggcga gccggtcgtc tgggacgcgc
     8521 gcgcccgcct caaagcgccc gaaatcgact ccgacatgcg taacaagatt tcctacgcgc
     8581 gcgggcgcgt cgcaacgacc tactacagtc aggagcagac gggcggcgcc gcgcccttca
     8641 agaagactaa gagtcccatc ttcatcgcct cgcaggacgc cgagttccag cacgagacgg
     8701 gcgtcggcat ctacacgggc gccgcgcgcg cgtggcagga cgacaatttc gtcaaggccg
     8761 accgcctcac cctgcgccgc gagcagaagc gcatggaggg cgacggcaac gtccagagcg
     8821 cgctctatca ggcgcgacgc aaggacgccg ccggcaaccg ctcggtcgtc cccgtcttcg
     8881 ccacctccgc gcgcatgttc tacgcggacc cggagcgcct gctccactac gagacgagcg
     8941 tggacatcaa gcagggcacc gagcgcatca tggcgaacgc ggccgatgtc tacctccagc
     9001 ccgagaccta cgaggccgag cgcaccgtcg cgcagggcaa cgtcgtcgtc acgcagcccg
     9061 gccggcgcgg caccggcgac tgggcgcagt acaccgcggc cgacgaatcg gtagtgctca
     9121 cgggcaaccc cgcccgcgtc gaggacgccg ccaagggcga ctccgcgagc aaccgcatga
     9181 ccgtctacct gcgcgaagac cgcatcgtct ccgacgccgg cgagagcaaa caatccacgg
     9241 gccgcgtgcg ctcgtcgcac aagataaaga aacagtgata agtgatgagt gataagtgat
     9301 aagaaagaga aaagcggact ttcttatcac tcatcactta tccttttcac tgctctaaag
     9361 atgcagaccg aggagatcat cgagacgaga agcggcgagg cgccggcgga gaggagtgcg
     9421 ccggacgtgg gcgtgccgtc gccgactgcg ctcgccgcgt tcgggctggt gaagagctac
     9481 ggcaagcggc gcgtggttga cgaggtcagc ctgcacgtcg aacagtcgga agtggtcggc
     9541 ctgctcgggg ccaacggggc ggggaagacg acgaccttct acatgatggt cgggctggag
     9601 cagccgaacg cggggcgcgt catgctgggc aagcgggaga tcacgaagct tcccatgtac
     9661 ctgcgcgcgc gcctcggcgt cggatatctg ccgcaggagc cgtcgatctt ccgcaagctc
     9721 tcggtcgagg agaatcttct ggccgtgctc gaacacatgc cgatgcgtcg ccgcgagcag
     9781 ttgaagcggg tggaagaact cctcgaagag tttggcatcg cgcaggtcag gcgcacgcgc
     9841 ggcgaccgtc tctcgggtgg cgagcgtcgc cgcacggaga tcgcccgcgc catcgccacc
     9901 gacccgcagt acatcctgct cgacgagccg ttcgccggca tcgacccgaa ggccgttgac
     9961 gaactccaga acctgatcct ctatctgagc agccgcggca tcggcatcct tatcacggat
    10021 cacaacgtcc gcgagacgct gggcgtcacc aaccgcgcgt acatcatggc cgagggaaaa
    10081 attttccgcg cgggcgctcc tctggagttg acggacgacc cggaggtgcg gcgtctctac
    10141 ctcggcgaga agtttcggat gtgaggggtt tgcgcgctcc ggcgtaaaat cggttataat
    10201 ctcctcgtgg ggcgcgccgt gaagcgcgtg tccgttaga
//
LOCUS       DASVES010000333         6540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1118074, whole genome shotgun sequence.
ACCESSION   DASVES010000333 DASVES010000000
VERSION     DASVES010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118074"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VF240_13680"
     CDS             <1..219
                     /locus_tag="VF240_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176308.1"
                     /translation="MGGAGLLATKLDAPVVPVYIRGLYELKQSGRRGYALPGSVTIVF
                     GEPVKFGAQDDPAEVTREIERRVASLAR"
     gene            238..795
                     /locus_tag="VF240_13685"
     CDS             238..795
                     /locus_tag="VF240_13685"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX7176309.1"
                     /translation="MATIFSHAVAATALGKVYEGEPSPPRFWWLAAACSIIPDFDTFG
                     LIFGIGYGDMLGHRGFTHSLLFALLAGLAVASVFFRDVPRLSEHWWRLVLFFFVATAS
                     HGVLDALTNGGLGVAFFSPFDTRRYFFPWRPVEVSPIGLGFFGPQGFSVIRNEIVWIW
                     MPASLLVVAAWVWRRAFGAWWRGRG"
     gene            1044..2156
                     /locus_tag="VF240_13690"
     CDS             1044..2156
                     /locus_tag="VF240_13690"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX7176310.1"
                     /translation="MLYRSLLRPLLFRLPPETAHEWAVHALAVALRPAPLRRLAARRL
                     ALRPFGELRRFGLSFPNPVGLAAGFDKNGRVLGEMAALGFGFVEAGTVTHLPQPGNPR
                     PRLFRLPLDRALVNRQGFNNEGAAALAARIDGARPDCVLGVNIGKSRAVEIDDAVADY
                     LASFRIVWAHADYVAVNVSSPNTPHLRELQRADRLCALLGALQESNRELSEKNSRRPV
                     PVLLKLAPDLETGELELIVDVALRAGVAGLIATNTTTARAGLQTPAGVVSAAGDGGLS
                     GAPLRERSTRVVAQLHSLSRGRLTIIGVGGIFDAADAWEKICAGASLVQLYTGLVYRG
                     VRIAHDINEGLAALVARHGLSTLDDAVGCRAAELAV"
     gene            2280..3023
                     /gene="pyrF"
                     /locus_tag="VF240_13695"
     CDS             2280..3023
                     /gene="pyrF"
                     /locus_tag="VF240_13695"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7176311.1"
                     /translation="MSDGRGAKDKLIVALDVETAAEAREVFAALRDSAEMFKIGPHLF
                     TSAGPEIVREIVAAGGRVFLDLKFHDIPAVVAAAGREAARLGVSLFTVHAAGGAEMLG
                     RVAESVADTAGREGSERPRVLGVTVLTSADDATLAETGVGCGVGEQVARLAALADASG
                     LDGCIASPHEIALVRRTVTRPGFLVVTPGVRPRTAAHDDQRRVMTPEQAVLAGADYVV
                     VGRAILKAPDRARAAREIVEEMEAVSSEQ"
     gene            3124..3861
                     /locus_tag="VF240_13700"
     CDS             3124..3861
                     /locus_tag="VF240_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7176312.1"
                     /translation="MMPEYWKQGGGRPFVYVSGLDGTGKLFFRQADDLARDHTVISFP
                     LRGEGLYGMPQLVEDLSAVVRDAGFSRATFLGESFGGLLVMAAALAHPEMFERMILVN
                     TFPYFAQRARIHMGVALFRVVPYALLKAHRTRAARHVLFSSDVSEEDRKTFREHTRVV
                     PREGYISRLRAIRDTDLRPRLGEIQAPALVVAGTADRLLDSVGAAKMLAGRLPRARLK
                     LLPGTGHTALLSERVRVRDWLREFEDI"
     gene            3981..5621
                     /locus_tag="VF240_13705"
     CDS             3981..5621
                     /locus_tag="VF240_13705"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX7176313.1"
                     /translation="MNRRTMLLPAVALALVCLLHAAPSACAQTVAGLDPGPFSPTPYR
                     VGERLSYNVSFSNFPTAAHVELFIAGRVQQSGRDGLELRGHVETIGIVSAALYAVNND
                     YVSFIDTATGIPYRTGQVIREGSQSANMLGALNPASGPPVLSDARASAPTPGTFDLLS
                     AVYRLRALPLAQGAIYRLTVQSASITHEVELKVTGRELLKTTVGSSNAIVAQVRVPGN
                     RALNDYRIRIYFSDDELHLPLLVTARHPAGEIRAELTSVEMVTPQATTAQPAPETGAQ
                     VKPPAGVVVLPPQTGGGVVAPPTPRPTPTPVPSTAEAPAASSNLGPPSAPPFAVGEQL
                     NFNFYAGPNSQPIGTASFHVRSRGKYFGREGLLINGLMQTTGAGQTLFPVNDIITSYV
                     DALTLLPFRTELRIQEGPRRVNWVVSADQERGNALFDDGTRVEIQAGTHDLVSVIYAM
                     RSFDLTPGRQTRVALLVNKRARALSVASLRQGTIELGGQSIPAVELLLTTGEPRGDRF
                     ALRLWVSTDRRRLPLRLTAQTPLGVVRGDLAIIPVSVQ"
     gene            5850..6143
                     /locus_tag="VF240_13710"
     CDS             5850..6143
                     /locus_tag="VF240_13710"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEX7176314.1"
                     /translation="MKPEKHLAPAEAKVSSAHAPASMPLDALPRGVQARVLRMEGSGP
                     VAKRLMEMGIVPGAPVSVVRSAPLGDPIEIRVRGYHLALRRSEARTITVKSDE"
     gene            6258..>6540
                     /locus_tag="VF240_13715"
     CDS             6258..>6540
                     /locus_tag="VF240_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HEX7176315.1"
                     /translation="MSTILEAPTLTRKPDDFVATPADSHDARTLIVALAGNPNAGKTS
                     LFNALTGLRQKVANYPGVTVEKKTGRCKLAGGRWADVIDLPGTYSLISRS"
BASE COUNT         1005 a         2351 c         2066 g         1118 t
ORIGIN      
        1 atgggcggcg cggggttgct ggcgacgaag ctcgacgcgc ccgtcgtgcc cgtctatatc
       61 cgtgggcttt acgagttgaa gcagagcgga cggcgcggct acgcgctgcc gggcagcgtc
      121 accatcgtct tcggcgagcc ggtgaagttc ggcgcgcagg acgacccggc ggaggtcacg
      181 cgcgagatcg agcgtcgcgt cgcgtcgctc gcgaggtagg ccgctgtcgc taaccacatg
      241 gccacgatct tttcacacgc cgtcgccgcc accgcgctcg gaaaagttta cgagggcgag
      301 ccatctccgc cacgcttctg gtggctcgcc gccgcttgct cgatcatccc ggacttcgac
      361 acgttcggac tcatcttcgg cataggctac ggcgacatgc tcggccatcg cggcttcacg
      421 cactccctgc tgttcgcgtt gctcgccggc ctcgcggtcg cgtccgtgtt cttccgagac
      481 gtgccgcgac tctcggagca ttggtggcga ctggtgttgt ttttcttcgt cgccacggcc
      541 tcgcacggcg tgctcgatgc gctgacaaac ggcggcctcg gcgtggcctt cttctcgccc
      601 ttcgacacgc ggcgctactt cttcccgtgg cggcccgtcg aggtctcgcc catcgggctg
      661 ggcttcttcg ggccgcaggg cttcagcgtc atccgcaacg agatcgtctg gatttggatg
      721 ccggcgtcac tgctcgttgt agcggcgtgg gtttggcggc gggcgtttgg agcttggtgg
      781 cgcggtaggg ggtgaagttt gtttggctct gacttgagag tgcaggaagt cggactcacc
      841 gcagaggcgc agagggcgcg gagggggcgc cgcagggttg acgcttacgc tcagcgttga
      901 tgcgtcgcac tcaactttga catggcgtga ccattgcaca caccttctcc cgtctagttt
      961 ttgacacgat ttcggcggct ctgttacgct cgtcgccttc ggctcatctc gtaactgaaa
     1021 aatcagtcgc gtccgccctg tccatgctct accgctcact gctgcgcccg ctgctgttcc
     1081 gtctgccgcc cgagacggcg catgaatggg ccgtgcacgc gctcgccgtc gcgctccgcc
     1141 ccgcgcccct gcgccggctc gccgcgcggc gtctcgcgct ccgccccttc ggcgaactcc
     1201 gacgcttcgg tctctcgttc ccgaaccccg tcggcctcgc cgccggcttc gacaaaaacg
     1261 ggcgcgtgct cggagagatg gccgcgctcg gcttcggctt cgtcgaggcc ggcaccgtga
     1321 cgcacctgcc gcagcccggc aatccgcgcc cgcgtctctt tcgcctgccg ctcgaccgcg
     1381 ccctcgtcaa ccgacagggc ttcaacaacg agggcgccgc cgcgctcgcc gcacgcatcg
     1441 acggggcgcg acccgactgc gtcctcggcg tcaacatcgg caagagccgc gccgtcgaga
     1501 tcgacgacgc cgtcgccgac tacctcgcga gcttccgcat cgtctgggcg cacgccgact
     1561 acgtcgccgt caacgtcagc tcgccgaaca cgccgcacct gcgcgagctt cagcgcgccg
     1621 accgcctctg cgcgctcctc ggcgcgcttc aagagagcaa ccgcgagttg tctgagaaga
     1681 actcgcgccg gcccgtcccc gtgctcctga agcttgcgcc cgacctcgaa acgggcgagc
     1741 tggagttgat cgtggacgtg gcccttcgcg cgggcgtcgc ggggctgatc gccacgaaca
     1801 cgacgactgc gcgcgcgggt ctccaaacgc cggccggagt ggtcagtgca gccggcgacg
     1861 gcgggttgag cggggcccct ctgcgcgaac gctcgacgcg cgtcgtcgcc caactccaca
     1921 gtctctcccg cggacgcctg acgattatcg gcgtgggcgg catcttcgac gccgcggacg
     1981 cgtgggaaaa gatttgcgcc ggggcctctc tggtacagct ctacacgggt ctggtctacc
     2041 gaggcgtccg tatagcgcac gacatcaacg agggactggc cgcgctcgtc gcgcgccacg
     2101 gcctgtccac gctcgacgat gccgtgggct gtcgcgccgc ggagctggcc gtctgacgcg
     2161 cgcggccggc ggtcggcatg tccgcccgcc cgttttacat tcggcccgcg cggaaggtat
     2221 cgtgtctgct cgaagcgtaa tgttcgtaca tccatcccga caggcagggg cataaaggtt
     2281 tgagcgacgg cagaggcgcg aaagacaagc tgatcgtggc gctcgacgtg gagacggccg
     2341 ccgaggcgcg cgaggtcttc gcggccctgc gcgacagcgc cgagatgttc aagatcgggc
     2401 cgcacctctt tacatcggcc ggccctgaaa tcgtgcggga gatcgtcgcg gcgggcgggc
     2461 gcgtctttct cgatttgaag ttccacgaca tcccggcggt cgtcgccgcg gccgggcgcg
     2521 aggcggcgcg actgggcgtt tccctcttca cggtacacgc cgcgggcggc gcggagatgc
     2581 tcgggcgcgt cgccgaatcg gtggccgaca cggccgggcg ggaaggttca gaacggccgc
     2641 gcgtcctcgg cgtcacggtg ctgacgagcg cggacgacgc gacactcgcc gagacgggcg
     2701 tcggctgcgg cgtcggagaa caggtcgcgc gcctcgccgc acttgccgac gcgtccggcc
     2761 tcgacggctg tatcgcctcg ccgcacgaga tcgctctcgt ccgccgcacg gtcacacgtc
     2821 cgggctttct cgtcgtcacg ccgggcgtgc gcccgcgcac agcggcccac gacgaccagc
     2881 ggcgcgtgat gacgcccgag caggccgtcc tcgccggcgc cgactacgtc gtcgtcggcc
     2941 gcgcaattct gaaagcgccc gaccgcgctc gcgccgcgcg ggagatcgtc gaagagatgg
     3001 aggcagtaag cagtgagcag taagcagtga gcagcagaca gttacttgat tgttgtcgcg
     3061 cagctcacat ttcaactcaa ctttactgct cactgctcac tgcttaccgc ttactgttaa
     3121 cgcatgatgc cggagtactg gaaacaaggt ggcggccgcc cgttcgttta cgtctcgggt
     3181 ctggatggga cgggaaagct ctttttcaga caggccgacg acctcgcgcg cgaccacacg
     3241 gtcattagtt ttccgctgcg cggcgaggga ctgtacggga tgccgcagtt ggtcgaagac
     3301 ctctcggccg tggtgcgcga cgcgggcttt agccgcgcga cgttcctcgg cgagtcgttc
     3361 ggcgggctgc tcgtgatggc cgcggcgctc gcgcacccgg agatgttcga gcggatgatt
     3421 ctggtcaaca cgttccccta cttcgcgcaa cgagcgcgga ttcacatggg cgtcgctctc
     3481 ttccgcgtcg tcccctacgc gctcctgaag gcgcaccgca cgcgcgccgc cagacacgtg
     3541 ctcttcagct cggacgtgag cgaagaagac cggaaaactt tccgcgagca cacgcgcgtc
     3601 gtgccccgcg agggctacat ctcgcgcctg cgcgccatcc gcgacaccga cctccggccg
     3661 cgcctcggcg agatacaggc gcccgcgctc gtcgtcgccg gcaccgccga ccgcttgctc
     3721 gatagcgtcg gcgcggcaaa aatgctggcc ggtcgtctcc cgagggcgcg cctcaagctg
     3781 ctgcccggca cgggccacac ggcgctgctg tcggagcgcg tccgcgtccg cgactggctg
     3841 cgggagttcg aggacatttg acgagtgctt tcggaacgcg gatgcgcccg ccggcgtgta
     3901 agctgctcgc gcgtcggggt tagccgcacg ccgggggccg cgggccgtgg cggagaagag
     3961 tggaaaaggg gagccgactt ttgaaccgta gaacaatgct gctgccggcc gtcgcgctgg
     4021 ccctcgtgtg cctcttgcac gcggccccgt cggcgtgcgc gcagacggtt gcgggccttg
     4081 accccggccc gttctccccc accccgtacc gcgtcggcga gcgtctctcc tacaacgtct
     4141 ccttctctaa cttccccacg gccgcgcacg tcgaactctt catcgcgggc cgcgtccagc
     4201 aatcgggccg cgacggtctg gagctgcgcg gccacgtcga gaccatcggc atcgtcagcg
     4261 ccgccctcta cgccgtcaac aacgactacg tctctttcat cgacacggcg acgggcatcc
     4321 cctaccgcac cgggcaggtg atccgcgaag gctcgcagag cgctaacatg ctcggcgcac
     4381 tcaatcccgc gtctggccca cccgtgctct ccgacgcgcg ggcttccgcg cccacgcccg
     4441 gcacgttcga cctcctctcc gccgtctacc gcctgcgcgc gctcccgctc gcgcaagggg
     4501 ccatctaccg gctcaccgtg cagagcgctt ccatcacaca tgaggtcgag ttgaaggtca
     4561 cggggcgcga gctgctgaag acgaccgtcg gctcgtcgaa cgccatcgtc gcgcaggtgc
     4621 gcgtgcccgg caaccgcgct ctcaacgact accgcatccg catctacttc agcgacgacg
     4681 agctgcacct gccactgctc gtcacggcgc gccacccggc cggcgagatt cgcgccgagc
     4741 tgaccagcgt cgagatggtc acgccgcagg cgacgaccgc gcagcccgcc ccggagacgg
     4801 gcgcgcaggt aaagccgccg gcgggcgtcg tcgtcctccc gccgcagaca ggcggcggag
     4861 ttgtcgcccc cccgactccg aggccgaccc ccacgccggt gccgtccacg gccgaagcgc
     4921 cagccgcgtc ttcaaatctg gggccgccat cggcgccgcc cttcgccgtc ggcgagcagc
     4981 tcaacttcaa cttctacgcc ggcccaaact cacagcccat cggcaccgcc tccttccacg
     5041 tccgctcacg cggcaagtac ttcgggcgcg aggggctgct catcaacggg ctgatgcaga
     5101 cgaccggcgc gggccagacg ctcttccccg tcaacgatat catcacctct tacgtggacg
     5161 ccctcacgct cctccccttc cgcacggagc tgcgcattca ggagggaccg cgccgcgtca
     5221 actgggtggt ctcggccgat caggagcgcg gcaacgcgct cttcgacgac ggcacgcgcg
     5281 tcgagattca ggccggcacg cacgacctcg tctcggtcat ctacgccatg cgctccttcg
     5341 acctcacgcc gggccggcag acgcgcgtcg cgctcctcgt caacaaacga gcgcgcgcgc
     5401 tctccgtcgc ctcgctacgg caagggacaa tcgaactcgg cgggcagagc atcccggccg
     5461 tcgaactcct gctgacgacg ggcgagccgc ggggagaccg cttcgcgctg cgcctctggg
     5521 tcagcaccga ccgccgccgc ctccccctgc gtctgacggc gcagacgccc ctcggcgtcg
     5581 ttcgcggcga tctggctatc atccccgtct cggttcagtg acccgcgccc cgagtccata
     5641 gaacaatcaa ccctgcggcg caaactcttg cgccctgcgg cgcctccgcg ttgagtacaa
     5701 cgctcactac actcaactca aatatgaact aaacagcata aactcccggc aaaaatcccc
     5761 acccttgttg aaattgaatt ttattttcaa ctatgacata ctcctctggc tctacagagc
     5821 catctttgat tctttgaggc agtcgtccgg tgaaacccga aaaacatctc gcgccagccg
     5881 aggcaaaagt tagcagtgcc cacgccccgg cctccatgcc gctcgacgcg ctgccgcgcg
     5941 gcgtgcaggc gcgcgtgctt cgcatggagg gcagcggccc cgtggcgaag cgtctgatgg
     6001 agatgggcat cgtgccgggc gcgcccgtct cggtggtgcg ctccgcgccg ctcggcgacc
     6061 cgattgagat tcgcgtgcgc ggctatcacc tcgccctccg gcgcagcgag gcgcggacga
     6121 ttacagtgaa aagtgatgag tgatgagtga taagagctgt tagctgttgg cgattagctc
     6181 tcagccaaac agctaacagc taatcgctaa tagcctcttc ttatctctta tcactcatca
     6241 ctcatcactt atcccgcatg agtacgattc tcgaagcccc gaccctgacg cgtaagccgg
     6301 acgacttcgt cgcgacgccc gccgactcgc acgacgcgcg cactctcatc gtggcgctcg
     6361 ccggtaaccc gaacgcgggc aagaccagcc tcttcaacgc gctgaccgga ctccggcaga
     6421 aggtcgccaa ctaccccggc gtcaccgtcg agaaaaagac cggccgctgc aagctcgccg
     6481 gcgggcggtg ggccgacgtc atcgacctgc ccggcaccta cagcctcatc agccgctccc
//
LOCUS       DASVES010000334         6565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_792724, whole genome shotgun sequence.
ACCESSION   DASVES010000334 DASVES010000000
VERSION     DASVES010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792724"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1142)
                     /locus_tag="VF240_13720"
     CDS             complement(66..1142)
                     /locus_tag="VF240_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176316.1"
                     /translation="MRRNLTLLCMLWLALASASSANAATPISVYGAWHCGNDACTWAT
                     VRDMTDFDAKNRWLVDRADGSGQPSVNLVVLSFVQPLKLLNKTTDSQTLNGIPRGMTQ
                     EVVNYFKSRGIRVMLSIGGITYTNAWNQALAANPTQFGLNAAAAAQALGVGIEIDYEE
                     NTTPNLVALQAFIDAYRSVLPYDATGNNHAARLTIDLAAGDRWLIALTRKATADWLNT
                     TTPVLDYANAMVPSRQPNSAATAQADWQEHIDGKAQYNPPILPLAAAKFTGGLYIVTG
                     QSPAPECVNFNNSLQKSTGTYVQNVATKTGTTNGMLGYMFWASECPSTRRICTVPPNT
                     CEGGVGAGARTYNVPIPMPALRQN"
     gene            complement(1222..3363)
                     /locus_tag="VF240_13725"
     CDS             complement(1222..3363)
                     /locus_tag="VF240_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEX7176317.1"
                     /translation="MRVRKFVPAVLSLFCVLSASAQSTGAAATTRQHNLMPVPASVRF
                     NAGRLPITKSFTVAARGHVDERLRAGVERVLRRLEGRTVMELARGLAGDPAAATLVVE
                     AVGPGRAVPSVEEDESYTLDVSDTQAVLKAPTVVGVLRGLETFLQLLDSDRAGYFIPA
                     AQIQDRPRFPWRGLLIDVARHYEPLEVLKRNIDAMAAVKLNVLHWHLTENQGFRIESK
                     KFPRLHGMGSDGQFYTQTEARELIAYARERGIRVVPEFDIPGHATAWLVGHPELGSAP
                     GPYTIERKAGIFDPVLDPTREEVYKFLDTFLGEMAALFPDAYLHIGGDENEGKHWDAN
                     PRIQAFMKEKGLKDNHALQTHFNKRLLTILKKHGKIMMGWDEIFQPDLPNDVVIHSWR
                     GPASLAEAARKGYKGVLSAGYYIDLIFPASQHYAADPLPADSTLTAAEAAHVLGGEAT
                     MWSEWVTHETIDSRIWPRTAAIAERLWSPRAVTDVEDMYRRLAVTSVRLEELGLTHEK
                     NQAMMLRRLAQSRDTAALQTLVSVIEPVKEYRRYQQRPQTMLSPLSGLIDAARPDSDA
                     ARRFAASVDALLSDAPRFEARAQQTRDTLNAWRSAGPELEALMEKSPALQEARPLVKD
                     LSELGTMGLEALSFLTKGVAAPAEWREARLAALEQAAKPKAALEFAVIQSVRELVVAA
                     AESQRLTQMTPAEWKTQVKSLAAPPPKQTRR"
     gene            3588..6209
                     /locus_tag="VF240_13730"
     CDS             3588..6209
                     /locus_tag="VF240_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX7176318.1"
                     /translation="MPGLSFHAFSSAGEGRIPGVRVCVSGVLCLIALAACAANAQTHA
                     TAGGGRKTAILLHDGWTFREAGKDAWHAATVPGCVHTDLLSAGLIEDPFYRDNEKKLQ
                     WIGKTDWEYRTTFRVAPGLLARRRVELVFKGLDTYAEVALNGVVLLRADNMFREWRVD
                     AKPALKSGENVLRVVFRSPINEVLPRMKAVGYELPASNDQGEKTSPYTRKAPYQFGWD
                     WGPRFVTSGVWKAVVLEAWDAARVADFHVSQKNLTAAAAELSANVEVVSDTDARATVV
                     LEDAASGALLAEQAVSLRPGVNPFKLDFRVVRPRLWWPAGLGPQNLYHFRARLLVGAR
                     AVDGATTRTGLRTLELRQRPDRWGKSFEFVVNGVPVFAKGGNWIPADSFPTRVTRERY
                     RQLLTSARDANMNMLRVWGGGVYESDDFYELCDELGLMVWQDFMFACSMYPGDEAFLE
                     NVRAEAVDQVRRLRKYPSIVLWCGNNEVETAWHHWGWKQQLPAKLWDDYLKIFHDVLP
                     RVVAEHDSSRPYWPSSPSSNLEDDADSQKVGDVHYWGVWHAALPFAEYEKQHPRFMSE
                     YGFQSFPLIETVSAYTEPADRDIQSPVMLAHQKHPRGNQLIREYMLREFAEPTDFESF
                     LYVSQVLQAEGIRVGTEHFRRIRPRNMGALYWQVNDCWPVASWSGIDYFGRWKALQHY
                     ARRFYGEVLVSPHEEDGHVRVHVVSDRTRPASATLRVSLLDFAGQVLFEKQVAVAVAP
                     LSSEVYLSLPKEELLRGRDARKVFLHCELSENGSALASNRLFFDAYKNLQLPAPRIDV
                     HVRPKGRALRVSLRADSLARDVHLSAGRLEGVFADNFFDLLPGRAVEVEFRPRRPLGV
                     GELRRSLKVRSLADAFK"
BASE COUNT         1063 a         2017 c         2266 g         1219 t
ORIGIN      
        1 gaagaagcat caactctccg cgtgaactca gcgcactctg cgactcggcg gtgaggacaa
       61 cgcgctcaat tctggcgcag ggccggcatg gggatgggga cgttataggt gcgcgcgccc
      121 gcgccgacgc cgccttcgca ggtgttcggc gggacggtgc agatgcgtcg cgtggacggg
      181 cactccgacg cccagaacat gtagccgagc atgccgttgg tcgtccccgt tttggtcgcc
      241 acgttctgca cgtaagtgcc cgtgctcttc tgcaacgagt tgttgaaatt gacgcactcg
      301 ggggcgggac tctggcccgt cacgatgtag agaccgccgg taaacttggc cgcggccagc
      361 ggcaggatcg gcgggttgta ctgcgccttg ccgtcgatgt gttcctgcca gtcggcctgt
      421 gcggtggccg cgctgttggg ctggcggctc ggcaccatcg cgttggcgta gtctaagacg
      481 ggcgtcgtgg tgttcagcca gtcggccgtg gccttgcgcg tcagcgcgat gagccagcgg
      541 tcgccggcgg cgaggtcgat ggtcaggcgc gcggcgtggt tgttacccgt cgcatcgtag
      601 ggcagcacgg agcggtacgc gtcgatgaac gcctgcaagg cgacgagatt cggggtcgtg
      661 ttctcctcgt agtcgatctc gatgccgacg cctaacgctt gagccgccgc ggctgcgttc
      721 agaccgaact gcgtcgggtt ggcggcgagc gcctgattcc acgcgttggt gtaggtgatg
      781 ccgccgatgg agagcatgac gcggatgccg cggctcttga agtagttgac gacctcctgc
      841 gtcatcccgc gcgggatgcc gttcaaggtc tgtgagtcgg tcgtcttgtt gagcagcttg
      901 agcggttgga cgaaactgag cacgacgaga ttcacggacg gctggccgga gccgtccgcg
      961 cggtctacga gccagcggtt cttggcgtcg aagtccgtca tgtcgcgaac cgtggcccac
     1021 gtgcaggcgt cgttgccgca atgccatgcg ccgtagacgg agatgggcgt ggcggcattg
     1081 gcggacgaag ccgacgcgag tgctaaccac agcatacaca gtagcgtgag gttccttctc
     1141 attacattac tcccttctca ttcgtgcgtg gtgggtgcgc cgaaatccgc aacgcgcgcg
     1201 gacgggcgcg cggcgtcgcc gttaccggcg cgtctgtttc gggggcggcg cggccagact
     1261 cttgacctgt gttttccact cggccggggt catctgcgtc agacgctgtg actccgcggc
     1321 ggcgacgacc agctctctca cgctctggat gacggcgaac tccagcgcgg ccttcggctt
     1381 cgccgcctgt tcgagcgcgg ccagtcgcgc ctcgcgccac tcggcggggg ccgcgacgcc
     1441 cttcgtcagg aaggagagcg cttccagccc catcgtccca agctccgaca aatctttgac
     1501 gagcggccgg gcctcctgta gcgccggcga cttctccatc aaagcctcca gctcggggcc
     1561 ggcgctgcgc cacgcgttca gggtgtcgcg ggtctgttgc gcgcgggcct cgaagcgcgg
     1621 ggcgtcggag agcagagcgt caacgctggc ggcgaagcgg cgggccgcgt cgctgtcggg
     1681 gcgggcggcg tcgatgaggc cactgagcgg gctgagcatt gtctgcgggc gctgctgata
     1741 gcggcggtat tccttgaccg gctcgatgac ggagacgagc gtctgaaggg cggccgtgtc
     1801 tctgctctgt gcgagacgcc ggagcatcat cgcctgattc ttctcgtgag tgagccccaa
     1861 ctcttcgagc cggacgctcg tgacggcgag gcgtcgatac atgtcttcca cgtccgtcac
     1921 ggcgcgcggc gaccagaggc gttcggcgat ggcggccgtg cgcggccaga tgcgcgagtc
     1981 aatcgtctcg tgcgttaccc actcgctcca catcgtcgcc tcgccgccga ggacgtgggc
     2041 ggcttcggcg gcggtgagcg tgctgtcggc cgggagcggg tcggcggcgt agtgctgcga
     2101 ggcggggaag atgaggtcga tgtagtagcc ggcggagagc acgcccttgt agcccttgcg
     2161 cgcggcctcg gcgagcgagg cggggccgcg ccacgaatgg atgacgacat cattcggcaa
     2221 atcgggctga aagatttcgt cccagcccat catgatcttg ccgtgcttct tgaggatggt
     2281 gagcaggcgc ttgttgaagt gtgtttggag ggcgtggttg tccttgaggc ctttctcttt
     2341 cataaaggct tgaatccgcg ggttggcgtc ccagtgcttg ccctcgttct catcgccgcc
     2401 gatgtgcagg taggcgtctg gaaagagcgc ggccatctcg ccgaggaagg tgtcgagaaa
     2461 cttgtagacc tcctcgcgcg tggggtcgag caccggatcg aagatgccgg ccttgcgctc
     2521 gatggtgtac ggccccggcg cgctcccgag ttcgggatgg ccgacgagcc aggcggtggc
     2581 gtgccccgga atgtcgaact cgggcacgac gcggatgccg cgctcgcgcg cgtaggcgat
     2641 cagctcgcgc gcctccgtct gcgtgtagaa ctgtccgtcg gaacccatcc cgtggaggcg
     2701 cgggaatttt ttgctctcga tgcggaagcc ctgattttcg gtgaggtgcc agtgcaggac
     2761 gttgagcttg acggcggcca tcgcgtcgat gttgcgtttg agcacctcca acggctcgta
     2821 gtggcgggcc acgtcgatga gcagcccgcg ccacgggaag cgcgggcgat cctgaatctg
     2881 cgcggccggg atgaagtagc ccgcgcggtc gctgtcgagt agttggagaa aggtttcgag
     2941 tccgcgcagg acgccgacga ccgtcggagc cttgagcacg gcctgcgtgt cggacacgtc
     3001 gagcgtgtag gattcgtcct cctcgaccga ggggacggct cggcccggcc ccacggcctc
     3061 gacgacgagc gtggcggccg ccgggtcgcc cgccagacct cgcgccagct ccatcaccgt
     3121 gcgcccttcg agccgtcgca ggacgcgctc cacgccggcg cgcaaacgct catcgacgtg
     3181 gccgcgggcg gcgacggtga aggatttcgt gatggggagc cggcccgcgt tgaaacgcac
     3241 ggaggcgggc acgggcatca ggttgtgttg gcgtgtggtg gcggccgcgc ccgtgctctg
     3301 ggcggaagcg ctcaggacgc agaacagcga caggacggcg gggacgaatt tacgcactct
     3361 catgtgctcc tcgacagaat tagggttaag ccggttcggg atttgtaccg catagataga
     3421 agggattgtg agtttcgccg atgtgtgtgt gcgccgggcg taagttacgg tttcatactt
     3481 gaggttacag aataaacgcc ggtaaaagct ataacaggtt tcagtcgaat cttcttcgtc
     3541 ttcattcaag cgaaataagt gttgcgacgg caggcgtttt tcgtacaatg cccggactga
     3601 gtttccacgc tttttcgtcg gccggcgaag gtcgcattcc gggcgtgaga gtttgcgtct
     3661 cgggcgtgct gtgcctgatc gcgctcgccg cctgcgccgc caacgcgcag acccacgcga
     3721 cggcaggtgg gggccggaaa accgccatcc tgctgcacga cggctggacg ttccgcgagg
     3781 ccggcaagga cgcgtggcac gcggcgacgg tgcccggctg tgtccacacg gatttgctga
     3841 gcgcgggcct catcgaagac cccttctacc gcgacaacga gaagaagctc cagtggatcg
     3901 ggaagaccga ctgggagtat cgcacaacct tccgcgtcgc gcccggactg ctcgcgcgcc
     3961 ggcgcgtcga gctggtcttc aagggtctgg acacctacgc cgaagtcgcg ctcaacggcg
     4021 tggtgctgct gcgcgccgac aacatgttcc gcgagtggcg cgttgacgcg aagcccgcgc
     4081 tcaagagcgg cgagaacgtg ctccgcgtcg tcttccgctc gcccatcaac gaggttctgc
     4141 cgcggatgaa ggccgtgggc tatgaactcc cggcgagcaa cgatcagggt gagaagacga
     4201 gtccctacac gcgcaaggcg ccctaccagt tcggctggga ctgggggccg cgcttcgtca
     4261 cgagcggcgt gtggaaggcg gtcgtgctcg aagcctggga cgcggcgcgc gtcgcggact
     4321 tccacgtctc acagaaaaac ttgacggcgg cggcggccga attgagcgcc aacgtggaag
     4381 tggtctccga cacggacgcg cgggccacag ttgtgctcga agacgcggcg agcggcgcgc
     4441 tcctcgccga gcaggcggtg agcctgcggc cgggcgtgaa tccgttcaag ctggattttc
     4501 gcgtcgtgcg cccgcgcctg tggtggccgg cggggctggg gccgcagaac ctctaccact
     4561 tccgcgcgcg tctcctcgtc ggcgcacgcg ccgtggacgg tgcgacgacg aggactggct
     4621 tgcgcactct ggagttgcgg cagcggccag accgctgggg aaagagcttc gagttcgtcg
     4681 tcaacggcgt ccccgtcttc gccaagggcg gcaactggat tccggccgac agttttccga
     4741 cgcgcgtgac gcgcgagcgt tatcgccaac tgctcacctc ggcgcgcgac gccaatatga
     4801 acatgctgcg ggtgtggggc ggcggcgtct acgagtcgga cgacttctac gagctgtgcg
     4861 acgagctggg gctgatggtc tggcaggact tcatgttcgc gtgcagcatg tatccgggcg
     4921 acgaggcttt tctggagaac gtccgcgccg aggccgtcga tcaggtgagg cgtctgcgaa
     4981 aatatccgag catcgttttg tggtgcggca acaacgaggt cgagacggcg tggcaccact
     5041 ggggctggaa gcagcagttg cccgcgaagc tgtgggacga ctacctgaag atttttcacg
     5101 acgtgttgcc gcgcgtcgtc gccgagcacg attcgtcgcg cccctactgg ccgagttcgc
     5161 cgagttccaa tcttgaagac gacgctgatt cacagaaagt cggcgacgta cactactggg
     5221 gcgtgtggca cgcggcgctg cccttcgccg agtatgagaa gcaacacccg cgcttcatga
     5281 gcgagtacgg cttccagtcg ttcccgttaa tcgagacggt gagcgcctac accgagccgg
     5341 ccgaccgcga catccagtcg cccgtgatgc tcgcgcatca gaagcacccg cgcggcaatc
     5401 agctcatccg cgagtacatg ctgcgcgagt tcgcggagcc gacagacttc gagtcgttcc
     5461 tctacgtgag tcaggtcttg caggccgaag gtatccgcgt cggcacggaa cacttccgcc
     5521 gcatccggcc gcgcaacatg ggcgcgctct attggcaggt gaacgactgc tggcccgtcg
     5581 cctcctggtc agggatagac tacttcggcc gctggaaggc gctccaacac tacgcgcgcc
     5641 gcttctacgg cgaagtgctc gtcagcccgc acgaggagga cggccacgtc cgcgtccacg
     5701 tcgtctccga ccgcacgcgc cccgcgagcg ccacgctccg cgtgagtctt ctggacttcg
     5761 cggggcaagt gctgtttgaa aaacaggtcg cggtcgccgt cgctccgctg tcgagcgagg
     5821 tctatctgag tttgccgaaa gaagagctgc tgcgggggcg ggacgcccgg aaagtttttc
     5881 ttcactgtga gctgtcggag aacggctcgg cgctcgcgag caaccgcctg ttcttcgacg
     5941 cttacaagaa cctacagctc cccgcgccgc gcatcgacgt tcacgtcaga cccaaggggc
     6001 gcgcgctacg cgtctccctg cgcgctgact ctctcgccag agacgtacac ctctccgccg
     6061 gccgactcga aggcgtcttc gccgacaact tcttcgacct ccttccgggc cgcgccgtcg
     6121 aagtggaatt ccgcccgcga cgcccgctcg gtgttggtga gctgcgaagg agtctgaagg
     6181 tgcgctcgct cgccgacgcc ttcaagtgaa gaattaacat ggatggacag gatggacagg
     6241 agtaagagag atagcagatt tcagatttca gatttgaaat tatcaagact gttcatcctg
     6301 cataccctgt ccatccatgt taataattct ttcgccgaga cgggccgcgg acttgtcttg
     6361 cccctacagt ttgaaggaaa actgacgctg tgaactcact caagatcggc atcggcggcg
     6421 tgcgcggggt ggtgggcgag acgtttacgc cggagctggc ggtggcgttc gcgcaagcct
     6481 tcggcacgta cctcgacgcg gggcgcatcc tcgtctgccg cgacacgcgc tcctcggggc
     6541 cgatggttca cgcggcggtc gtctc
//
LOCUS       DASVES010000335         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1592269, whole genome shotgun sequence.
ACCESSION   DASVES010000335 DASVES010000000
VERSION     DASVES010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592269"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VF240_13735"
     CDS             <1..1011
                     /locus_tag="VF240_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7176319.1"
                     /translation="RIDELTAADWGMQVADVLHYLHSQRPPIIYRDLKPANLMIDGNS
                     GRVMLIDFGIARWVQKEEKGVTAVGTMGYAPPELFSGKAEARTDIYSLGATLFHLLTG
                     SDPQDNPLLIFDFTKNPRPRQINPSISNEMERILMRAVEYSPEGRFRSAAEMRDGLSL
                     HLEKLRAGAVTYGAAIYPGRESAQTIQQEMVYCGFCGGRIAGDDVFCAHCGARQPIAS
                     TQAAVRIARATAQLVVMGTTELEGTFHLQKESNLVGRTDPQSNIFPDVDLSRFDQETK
                     ISRRHARIWRQGDTYLVEDLGSVNGTVVNDSVRLGPRQPRTLENGDKLRLGETTLHFL
                     VG"
     gene            1228..1767
                     /locus_tag="VF240_13740"
     CDS             1228..1767
                     /locus_tag="VF240_13740"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7176320.1"
                     /translation="MTARIEHSPTAAHSAATSEARPAARTTEARAERLRAPFSLRCGA
                     LLIDYTLPVAVIALFTLFARSFDANTRWFGVGLVTLGYVLGVALAVFNLFVLTVITGR
                     TAGKWVTDLRIERRDGTPLSYGRAVLRHLVGYALTLCTLGVGFLLAAFNPEGRALHDL
                     VAGTVVVRRRSVARTRRPR"
     gene            1910..3700
                     /locus_tag="VF240_13745"
     CDS             1910..3700
                     /locus_tag="VF240_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176321.1"
                     /translation="MNRICATCAAELAEGARFCRQCGSPLVSATGRDGGEQVSPQAAT
                     IPLSEEGRTTDGLAGEDPSRRAAPETTRVSRAEMDSILQRAARDTGAGVDPNQTNAQS
                     SGLSQRAAPAEEDLLKTRASFSEEDLMKTRPSVSAAPASSSPPAVPSSPQLTPSSSPR
                     PAPSEEDLMRTRPSLPADFDEELTISVPRPFTGDINPVPVQPTPVTGPLAQRREAGPN
                     AAAAAQPLAPAAPAGQQAPPARRRGPWIIVAVVCAVLLVVGVVGALLAVNYLRRPATD
                     SATVAPVAPVAPDQRQLAEEKVVEAEALLASGDLSGAVERLREAVRLDPNHTSAHRRL
                     GDILLETGARREAIEELRAVTNLEPNDFTAWRALAAAQLAEGLPADAAESYKRLVALT
                     GETEPNDLLAYADALRLSGRAEEANTLYQRLSTSTSADVAAAARQRLSELASAVTAPT
                     PTATREARPNGDANANTSAGTQTTTASNLPAPTPAAQPTAAPAPQPTPPPAQLTPAER
                     YRRGVELWGGNRGAALNEFRAAAAAGISDANYYLGLSLVEGRDLSTLKRAEVVSALGH
                     FQRAQSGSNGAQARRYVQQLEKEYDRIRSQ"
     gene            3774..>4234
                     /locus_tag="VF240_13750"
     CDS             3774..>4234
                     /locus_tag="VF240_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176322.1"
                     /translation="MKNSALRLIVALITFAVGIALVTLWLGYKDAGHTRRAKTPCRLR
                     ARTPPPPAAASGAESPAGESLLPVLSYCELVGNPERYNGKVVRVRAALRMSIHGLFLS
                     DVTCDRYEDYAAVAFDEPRGEELMDAFFGTDGPVRVGRGVVELIAVGRFYKV"
BASE COUNT          689 a         1554 c         1341 g          650 t
ORIGIN      
        1 cgcatcgacg aactcacggc cgccgactgg ggcatgcagg tcgccgacgt tctgcactat
       61 ctccacagcc agcgcccgcc gatcatctat cgcgatttga agcccgcgaa cctgatgatc
      121 gacggcaatt cggggcgcgt catgcttatc gacttcggca tcgctcgctg ggtgcagaag
      181 gaagagaagg gtgtcacggc cgtcggcacg atgggctacg ccccgcccga actcttcagc
      241 ggcaaggccg aggcgcgcac ggacatctac tctctgggcg cgacgctatt tcatctgctc
      301 acgggatcag acccgcagga caacccgctg ctcatcttcg acttcacgaa gaacccgcgg
      361 ccccggcaga tcaacccgtc gatctcgaac gagatggagc gcatcctgat gcgcgccgtc
      421 gagtacagcc ccgagggtcg cttccgctcg gccgccgaga tgcgcgacgg gctctcgctc
      481 catctggaaa agctgcgcgc gggggccgtc acctacggcg cggccatcta tccgggccgc
      541 gagtcggcgc agaccatcca gcaggagatg gtctactgcg gtttctgcgg cggtcgcatc
      601 gcgggcgacg acgtgttctg cgcccactgc ggcgcgcgtc agcccatcgc cagcacgcag
      661 gccgccgtcc gcattgcgcg cgccaccgcg caactcgtcg tgatgggaac caccgagctt
      721 gaagggacgt ttcatctcca gaaggagagc aatctggtgg ggcgcaccga cccgcagtcg
      781 aacatctttc cggacgttga tctgtcgcgc ttcgatcagg agacgaaaat ctcgcgccgc
      841 cacgcgcgca tctggcgaca gggcgacacc tatctggtcg aagacctcgg ctcggtcaac
      901 ggcacggtcg tcaacgacag cgtgcggctc ggcccccgcc agccgcgcac gctcgaaaac
      961 ggcgacaagc tgcgtctcgg cgagacgacg ctgcactttc tggtggggta gctaacagct
     1021 attagctgtt agctattagc cgttagctaa aagctgcccg tggtcagtag tccgttgtcc
     1081 tttgttcgct tcgggcaact cgtcgcacac acatcaaggc tcaacgcggg caaccgaaaa
     1141 caactgacaa ttgaccactg accactgacc actgaccagg gataaagaac agctcttagc
     1201 taacagctaa cggctaacag cttccccatg accgcgagaa tcgaacacag tcccactgcc
     1261 gcccactcgg ccgccacgag cgaggcccgg cccgcggcgc ggacgacgga ggcgcgcgcc
     1321 gagcgcctgc gcgcgccctt ctccctgcgt tgtggtgcgc tcctcatcga ctacacgctg
     1381 cccgtcgccg tcatcgccct gttcacgctg ttcgcgcgca gcttcgacgc gaacacgaga
     1441 tggttcggcg tcgggctggt gacgctcggc tacgtgctgg gggtggccct tgccgtcttc
     1501 aacctcttcg tgctcacggt gattacgggc cgcacggcgg ggaagtgggt gaccgacctg
     1561 cgtatcgagc gccgcgacgg cacgccgctc tcctacggcc gcgcggtgct ccgccacctc
     1621 gtcggctacg cgctgaccct ctgcacgctc ggggtcggct tcctcctcgc cgcgttcaac
     1681 ccggagggcc gggcgttgca cgacctcgtc gccggcaccg tcgtcgtgcg ccggcgcagc
     1741 gtcgcgcgca cgcgccgccc tcgctgaaca agccgtcagt tattagctat tagcagttag
     1801 ccagacataa atccgagcac gttcccgctg atggataaat ctctggttcg cactcttctg
     1861 gctgaccgct aatagctaat agctgatagc ttctgacgaa ggtattcgga tgaatagaat
     1921 ctgtgcaact tgcgcggccg agttggcgga gggcgcgcgc ttctgccgcc agtgcgggtc
     1981 gcctctggtg agcgcgacgg gccgcgacgg tggcgagcag gtctcgccac aggcggccac
     2041 gatccccttg agcgaggagg ggcgcacgac cgacgggctg gcgggcgaag acccgtcgcg
     2101 ccgcgccgcg cccgagacga cgcgcgtcag ccgcgccgag atggactcca tccttcagcg
     2161 cgccgcccgc gacaccgggg cgggcgtaga cccgaaccag accaacgcgc agtcgtccgg
     2221 tctctcacaa cgcgccgcgc ccgccgaaga agaccttctg aagacacgcg cctccttcag
     2281 cgaagaggat ttgatgaaga cgcgcccttc ggtctctgcc gcgcccgctt cgagttcgcc
     2341 gcctgccgtc ccttcaagtc cgcaactcac gccctcttcg agtccgcggc ccgcgccctc
     2401 ggaggaagac ttgatgcgga cgcggccgtc gctccccgcg gactttgacg aggagctgac
     2461 gatcagcgtg ccgcgcccct tcacgggcga catcaacccc gtgcccgtgc agccgacgcc
     2521 cgtcacgggg ccgctcgccc agcggcgcga ggccgggccg aacgccgccg ccgccgctca
     2581 gccgcttgcg cctgccgcgc ctgccggcca acaggctccg cccgcgcgcc ggcgcggccc
     2641 gtggataatc gtcgccgtcg tgtgcgcggt gctgctcgtc gtcggggtcg tcggcgcgtt
     2701 gctcgccgtc aactacctgc gccgccccgc gaccgactcg gccacggtcg cccccgtcgc
     2761 gcccgtcgct cccgaccagc ggcagctcgc cgaagagaag gtcgtcgagg ccgaagccct
     2821 cctcgcctcc ggcgacctga gcggcgcggt cgagcgtctg cgcgaggccg tccgcctcga
     2881 cccgaatcac acgagcgcac atcgccgcct cggcgacatc cttctggaga ccggcgcgcg
     2941 gcgcgaggcc atcgaggagc tgcgcgccgt cacgaatctg gagccgaacg acttcacggc
     3001 ctggcgtgct ctcgccgcgg cgcagttggc cgaggggctg cccgccgacg cggccgagag
     3061 ctacaagcgt ctcgtcgcgc tgaccggcga gacggagccg aacgacctgc tcgcctacgc
     3121 cgacgcgctt cgcctctcgg gccgcgccga ggaggccaac acgctctatc agcgcctctc
     3181 cacttccacg tcggccgacg tggcggccgc cgcccgccag cgcctctcgg aactcgcctc
     3241 ggccgtgacg gcgccgacgc caactgccac acgcgaagcg cgcccgaacg gcgacgccaa
     3301 cgccaacacc tccgccggca cgcagacgac caccgcctcg aatttacccg cgccgacgcc
     3361 cgccgcgcag cccacggccg cgccggcgcc gcagccgacg cccccgcccg cgcagttgac
     3421 gccggctgaa cgctaccgcc gcggcgtcga gctgtggggc gggaaccgcg gcgcggccct
     3481 caatgaattt cgcgcggccg ccgccgccgg catctccgac gccaactact acctcggcct
     3541 cagcctcgtc gaaggccgcg acctcagtac cctgaagcgc gccgaagtcg tctccgccct
     3601 cggtcatttc cagcgggcgc agtccggcag caacggcgcg caggcgcggc gctacgtgca
     3661 gcagctcgaa aaggagtatg accgcatcag gagccagtag cggaagccgc cgctgaagac
     3721 ggccgcgcgg aactgttctc gccgctccgg tgtccaagtt gcggaggtga tttatgaaga
     3781 actcggcact ccgactcatc gtcgcgctca ttactttcgc cgtcggcatc gcgctcgtca
     3841 cgctctggct cggatacaag gacgccggcc acacgcgacg cgcaaagacg ccctgccgtc
     3901 tgagagcgcg aacgccgccg ccgccggccg ccgcgtcggg cgcggagtcg ccggccgggg
     3961 agagcctttt gcccgtcctc tcctactgcg agcttgtggg caaccccgaa cgctataacg
     4021 ggaaggtcgt ccgcgtccgc gccgcgcttc gtatgtccat ccacggcctc ttcctgagcg
     4081 acgtgacgtg cgaccgctac gaagactacg cggcggtagc cttcgacgag cctcgggggg
     4141 aggagttgat ggacgccttc ttcgggacgg acggccccgt gcgcgtcggg cgaggcgtcg
     4201 tcgaattgat cgccgtcgga agattctaca aggt
//
LOCUS       DASVES010000336         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1182282, whole genome shotgun sequence.
ACCESSION   DASVES010000336 DASVES010000000
VERSION     DASVES010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182282"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..2169)
                     /locus_tag="VF240_13755"
     CDS             complement(97..2169)
                     /locus_tag="VF240_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176323.1"
                     /translation="MTRTPRTTLALYALVVISAVLYYCGLAPAGAAPQAGIENIDNRE
                     SFTTAASGLTTIDFSGVAPARGFGKYRPEEGLSAGGATFRARGGARFGPGLIYVISAH
                     YGGMNPLHNTGMLPHLSWGAPNQPGNASLDVSLPAGTTAVGCDLWTQQPYVSTIEVTA
                     TMADGRTHTSVVNTRARPAAAFVGFISDTSIVSLSFRPPKGQAGLLLDNFAYGRRAEG
                     RRVQPTATAAAPKDADAAAPAQPSRPTTSTDAQSPASRPGQSASSNTRPTPDTPASQT
                     TTPSATQSPAPTATQSSSAPARGAIAYVRGGTEIRLVESDGSNDRRLWTHEYAKEGSS
                     IHELAWRPDGKELAFSSSHFNIASLYHADIYAVGPDGKGFRKITNAPDHGELARYPKG
                     AVSVTVRNEQPIWKQSRASTGVFFVYVVGADEPQAVTLPPGASKTLLFKSVADYGDHA
                     QPVVAMYGQYRWFIPGVDVRAGRTINAPAFGISGDGIPLFGAFRPVWRSDGSRLSYRD
                     GLCLINTIPAQPKPGELVFDKLLAGEQPSGACAWDWGPTPALADQIIYSANSDGEGTS
                     VHRVREGGAHPGTKLHAFSELDLQLLTDLRWLPDGSGFLYSFPDLAYEFGNIFRYDFA
                     TKRATQLTNFEGEYARAFDISPDGAWVVFERAKKLDDDKDVDLWVMRSDGREARLLVR
                     GGLAPSWR"
     gene            complement(2241..3566)
                     /gene="murA"
                     /locus_tag="VF240_13760"
     CDS             complement(2241..3566)
                     /gene="murA"
                     /locus_tag="VF240_13760"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7176324.1"
                     /translation="MDKFRITGGNPVRGRVSISGAKNSALPCLAAALLTPETVTLHNV
                     PYVRDCMTQRRLLEDLGATVLVPDLRTHKITASNIELFEAPYELVKTMRASVLALGPL
                     VARFGKAKVSLPGGCAIGTRPIDLHLRGLEGLGAEVSLEAGYVVARAPKGGRLRGGEI
                     TFEKVTVTGTENLMMAAALAAGTTVLHNAAREPEINDLADLLNRMGARVRGAGTPTLT
                     IEGVEHLGGAEHTIIPDRIETGTFLVAAAITGGELEVKDCRPEHSAAVIAKLREAGVE
                     IEEINPSTLRVRRSAEGLRARDITTEPHPRFPTDMQAQYMALMTQAEGASRVTETIFE
                     NRFMHASEMQRMGARIQIDGHTATVEGPTELTGARVQASDLRASACLVLAGLAARGET
                     IIDRVYHIDRGYEKIEAKLRSVGAQIERLVDSVTAPLPPIEAIGAADGV"
     gene            complement(3710..>3969)
                     /locus_tag="VF240_13765"
     CDS             complement(3710..>3969)
                     /locus_tag="VF240_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176325.1"
                     /translation="GLQREVREHEPHIALTPGGDGLRVVRRLVSESPRYLQPGGHLLF
                     EIGFNQHEQVERLIDARVWTLLRIHKDLQSIPRTVALRLRR"
BASE COUNT          588 a         1207 c         1491 g          683 t
ORIGIN      
        1 gccgctgtta ttgataatgg ttagaagagg cggttttgtt cggtcatgtg aaaatcagtc
       61 cgtgatgtcc gctaaagatt ttaccggcgc tggcgctcaa cgccacgacg gggcgaggcc
      121 gccgcgcacg agcagacgcg cctcgcggcc gtccgagcgc atcacccaga gatcaacgtc
      181 tttgtcgtcg tccagcttct tcgcgcgctc gaagacgacc cacgcgccgt cgggagagat
      241 gtcgaaggcg cgcgcgtact cgccctcgaa attggtgagc tgcgtggcgc gtttcgtcgc
      301 gaagtcgtag cggaagatgt tcccgaactc gtaggccaaa tccgggaacg agtagagaaa
      361 gccggagccg tcgggcagcc agcgcaggtc agtgaggagt tgaaggtcaa gctcgctgaa
      421 ggcgtggagt ttcgtgccgg ggtgcgcgcc gccctccctg acgcggtgga cgctggtgcc
      481 ttcgccgtcg gagttcgcgc tgtagatgat ctggtcggcg agcgctggcg tcggccccca
      541 gtcccacgcg cacgcgcccg acggctgctc tcccgcgagg agcttgtcga agaccagctc
      601 gccgggcttg ggctgtgcgg ggatggtgtt gatgaggcag aggccgtcgc ggtagctcag
      661 gcgcgagccg tcgctgcgcc agacggggcg gaaggccccg aagagtggga tgccgtcgcc
      721 gctgatgccg aaggccgggg cgttgatcgt ccgcccggca cgcacgtcca cgccggggat
      781 gaaccagcgg tactgtccgt acatcgcgac gacgggctgg gcgtggtcgc cgtagtcggc
      841 cacggacttg aacaggaggg tcttggaagc gcccggcgga agcgtcaccg cctgcggctc
      901 gtcggccccg acgacgtaga cgaagaagac gcccgtgctc gcgcgcgact gcttccagat
      961 gggctgctcg ttgcgtaccg tgacgctcac ggcgcccttc gggtagcggg cgagttcgcc
     1021 gtggtcgggc gcgttggtta tcttgcggaa gcccttgccg tcggggccga cggcgtagat
     1081 gtctgcgtgg tagagcgagg cgatgttgaa gtggctgctg gagaaggcca gctccttgcc
     1141 gtcgggcctc cacgccagct cgtgaatgga cgagccttct tttgcatact cgtgagtcca
     1201 gagtcgccgg tcgtttgagc cgtccgactc gacgagacga atctcggtgc cgccgcggac
     1261 gtaggcgatg gcgccgcgcg cgggcgcgga ggaagactga gtcgccgtcg gagcgggaga
     1321 ctgagtcgcc gacggggtcg tggtctgact cgctggcgtg tcgggagttg ggcgcgtgtt
     1381 ggaagaggcc gattggccgg gccgcgacgc gggcgattga gcgtcggttg aagtggtcgg
     1441 ccgcgaaggc tgcgccggcg cggctgcgtc cgcgtctttg ggcgcggcgg ccgtcgcggt
     1501 gggttgcacg cggcggcctt cggccctgcg gccgtaggcg aagttgtcga ggagcaggcc
     1561 cgcctgcccc ttcggcgggc ggaagctcag ggagacgatt gacgtgtcgg agataaagcc
     1621 gacgaaggcg gcggcggggc gcgcgcgcgt gttgacgacc gaagtgtgcg tccgcccgtc
     1681 ggccatggtg gcggtgactt cgatggtcga gacgtatggc tgctgcgtcc agaggtcaca
     1741 gccgacggcc gtcgtgccgg cgggcaagct gacgtcgagc gaggcgttgc cgggctggtt
     1801 cggcgcgccc caactgaggt gcggaagcat gcctgtgttg tggagcgggt tcatgccgcc
     1861 gtagtgggcg ctgatgacgt agatgaggcc cggcccgaag cgcgcgccgc cgcgtgcgcg
     1921 gaaggtcgcg ccgcccgccg acagcccctc ctccggccgg tacttgccga agccgcgcgc
     1981 cggcgcgacc ccgctgaagt cgatggtcgt gaggcccgag gcggcggtcg tgaagctttc
     2041 gcggttgtcg atgttttcga tgccggcctg cggcgcggcg ccggcggggg cgaggccgca
     2101 gtagtagagc acggcggaga tgacgacgag ggcgtatagg gcgagggtcg tgcgaggcgt
     2161 gcgtgtcacg ttcttctact cctccttgtg aaattgtcgt ccggggtcgc gttgggaaga
     2221 gtttgtcaac agagctgtgt tcagactccg tccgccgccc cgatggcctc gatgggtggg
     2281 agcggcgcgg tgacggaatc cacgaggcgc tcgatttgcg cgccgaccga gcggagcttg
     2341 gcctcgatct tctcgtagcc gcggtcgatg tgatagacgc ggtcgatgat ggtctcgccg
     2401 cgggcggcga gtccggcgag gacgagacag gccgaggcgc gcaggtcaga cgcctgcacg
     2461 cgcgcgcccg tcagctccgt cgggccttcg accgtggccg tgtggccgtc gatctggatg
     2521 cgcgcgccca tacgctgcat ctcggaggcg tgcatgaagc gattctcgaa gatggtctcg
     2581 gtgacgcgcg acgcgccttc ggcctgcgtc atcagggcca tgtactgcgc ctgcatgtcg
     2641 gtgggaaaac gcgggtgcgg ctcggtggtg atgtcgcgcg cgcgcaggcc ctccgcggag
     2701 cggcgcacac gcagcgtgct cgggttgatc tcctcgatct cgacaccggc ctcgcgcagc
     2761 ttggcgatga cggcggccga gtgttcgggc cggcagtctt tcacttcgag ttcgccgccg
     2821 gtgatggcgg cggcgacgag aaacgtgccc gtctcgatgc ggtcggggat gatggtgtgc
     2881 tcggccccgc cgaggtgttc gacgccctcg atggtcagag tcggcgtgcc cgcgccgcgc
     2941 acgcgcgcgc ccatgcggtt gaggaggtcg gcgaggtcgt tgatctccgg ctcgcgcgcg
     3001 gcgttgtgaa gcacggtcgt cccggcggcg agcgcggcgg ccatcatcag attctccgtg
     3061 cccgtcaccg tcaccttctc gaaagtgatt tcaccgccac gcaggcggcc ccctttcggc
     3121 gcgcgcgcga cgacgtagcc ggcttccaga gagacctccg cgcccaagcc ttcgaggccg
     3181 cgcaggtgga ggtcgatggg gcgcgtaccg atggcgcacc cgcccggcag actcaccttc
     3241 gccttgccga agcgcgcgac gagcgggccg agcgcgagca cggaggcacg catcgtcttg
     3301 accaactcgt agggcgcttc aaacagctcg atgttcgagg ctgtgatctt gtgcgtgcgc
     3361 aggtcgggca ccagcacggt cgcgccgagg tcttcgagca ggcgtcgctg cgtcatgcag
     3421 tcgcgcacgt aagggacgtt gtgcagcgtc acggtctcgg gcgtcaaaag cgccgccgcc
     3481 agacacggca gcgccgagtt cttcgccccg ctgatggaga cgcgacccct tacgggattg
     3541 ccgccggtga tacgaaattt gtccataaag aattagaggt cggaggtcag aggttagagg
     3601 ttagtgaaga cagtttttaa ctgaccactg atctctgacc tccgacctct gaattttggc
     3661 gattctagca cgcgccgcga agcgcaatct agcccgaggc gcggcgcgct caacggcgca
     3721 ggcgcagcgc gacggtgcgg gggatgcttt gcaggtcttt gtgaatacgc aggagcgtcc
     3781 agacgcgcgc gtcgatgagg cgctccacct gctcgtgctg gttgaagccg atttcgaaga
     3841 ggaggtggcc gccgggctgg aggtagcgcg gcgactccga gacgaggcgg cggacgacgc
     3901 gcaggccgtc gccgccgggc gtgagcgcga tgtgcggctc gtgttcgcgc acctcgcgct
     3961 ggagtcccg
//
LOCUS       DASVES010000337         7444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_777544, whole genome shotgun sequence.
ACCESSION   DASVES010000337 DASVES010000000
VERSION     DASVES010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777544"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF240_13770"
     CDS             complement(<1..306)
                     /locus_tag="VF240_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176326.1"
                     /translation="MSARTLFVVVLALLATAGPFDLKPALHSPLELKAAAAHRRGAED
                     AEGAQRVPAAQPTPTPSPTEAPDDEVERVDVDLASVLLTAVDRERRFVTTLRREDIRL
                     "
     gene            complement(353..2116)
                     /locus_tag="VF240_13775"
     CDS             complement(353..2116)
                     /locus_tag="VF240_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7176327.1"
                     /translation="MKIRVKPLLLIPLLLAAGVCARAQDGGFERVLAASGPINLSVKN
                     RLGRVTVTAVEGEEKKVTIRARSPLSGVTVEERDVKIEEAGARLSVVVERESKASAGV
                     GAGGEANRNRIDVVLTVPARTRVEVETEVGAVDVVGPLAEARVTTGTGTIRADVPTDA
                     VRYSLRWTLARPRVYSEVTLGKVKEQRSGAFEISGRLGDQKAKKDARIELSLETARGV
                     ILFGVADAASVPSDLRERELTEAARAVIRSGDADLTDAIRKLVPRLVGEYAETLPPTR
                     FEPTFAARRNPFDVRTSLGAELARLNASVTDRNGRAVGGLSARDFTVVENGEERKVRA
                     VEPSTAPFNLVLLLDVSGSVEERLDFIRKAALAFVETVGPTDRVAITSFRDDVQVISE
                     FTTDRAALARRIKDIQAGGATSLYDALAYTLVDTLKPLRGERTAVVILSDGDDNRSFI
                     PFNELLEAVLESGALVYPLYVPSGLIPSSSAAAPASTIDPMRSRFLTLTSRAREEGRR
                     LAELSGGVYYPITRIEQLQRAYDDVVAQLRTSYSITYESSAGAAAVTGARVRVRVARE
                     GASVRLSPSVGVNNNSPTAAP"
     gene            complement(2113..2328)
                     /locus_tag="VF240_13780"
     CDS             complement(2113..2328)
                     /locus_tag="VF240_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176328.1"
                     /translation="MHLESKSAASVSPALVFELDNGVNYTFNFQMDGRVMLLGFQEGR
                     AVAGSFTEEQITQLRDAITAYLDKRTG"
     gene            2642..3658
                     /locus_tag="VF240_13785"
     CDS             2642..3658
                     /locus_tag="VF240_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX7176329.1"
                     /translation="MKNKILISLLVALALSAVAAFAALPGRGQSQAAGAQQQQAPSSA
                     QDEQAGAAASQTGVKLTASIHERRPAAAVVTGDLSAFAASASKNLSLKYDLTWAFGGK
                     QQRGWYLYASLIGSLLDTNDETGSAGFAAALSRWQRRAGVAPTGILDDETLYRMVSDW
                     QGARIKSRAYPQPSELVTAPPTEFWDPSRPDELRQVRRDAYDAYKRMLAAAVADPSLK
                     LAVTRTGQLAPEEKFLKIVSSFRSREYQEQLRKRQPNAGRAGLAVNSPHFTGCALDLY
                     VGGDPVDTKDANRAIQVQTPVYQWLVRNAGKFGFRPYYYEPWHWEYVGESKAGGVELV
                     GVMK"
     gene            complement(3785..4342)
                     /locus_tag="VF240_13790"
     CDS             complement(3785..4342)
                     /locus_tag="VF240_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176330.1"
                     /translation="MRKTLNGSGLIAALALALALAGCGGGGAGAGAGASSKHPGTDDG
                     AKALLAEFVKPGADHATLSKTLRPSKADYDAVFLPDLAAKADTVYGPAWDGGQMVVAP
                     KEGQTQVLTSSATSDELKSWTGGAAEFPGGWKDVGAQLKPGVKFYRFKFVEPGKDLGM
                     AYDGLAYVNGNWRIFPKPWRVMGGS"
     gene            complement(4398..4964)
                     /locus_tag="VF240_13795"
     CDS             complement(4398..4964)
                     /locus_tag="VF240_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176331.1"
                     /translation="MKTSVRLAAAALFMSACLSGCGDSERYKAEEKFGPINKKMMAAV
                     GDLTAVPAKTELVAEPYVKGKIAVFQARGSKGSAARDNTYFMQIDYFRELEAFYAATP
                     EEVGTVALMHCKTTQKGTYKTDDGKEFPAEAEDCELTMVDRSKAAVVYKKLFEKTPSQ
                     ERKAYGNTMVRESAQTDILQFLKGLPRT"
     gene            complement(5140..5325)
                     /locus_tag="VF240_13800"
     CDS             complement(5140..5325)
                     /locus_tag="VF240_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176332.1"
                     /translation="MTAFWIVLSIMLFAGAELGAGGNFGDTPGLNGGGDAVCTSSARR
                     QCDEQRLGGGEGGWPGF"
     gene            complement(5484..6185)
                     /locus_tag="VF240_13805"
     CDS             complement(5484..6185)
                     /locus_tag="VF240_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176333.1"
                     /translation="MPLLFMFVPPGQEGALTDNGSSVVVTSFKWSKSRQTTEVAEAAS
                     TAPAAAMISTNKNYERGRRANSSAGVANVPAGVSNPTGDTVDSRSAELERNVREARSP
                     KRKAVDGFAYRVKVRNTSAKVIEVVFWEYRFTESANPTNVARRQFLCGVNIKPAREKE
                     LQAFSPSGPGAVISVGSLSDKTGNLYQEQVVINRVEYADGSIWQRKDWNFAEVRLTLA
                     RAIETPWGLEMCRGL"
     gene            6522..7286
                     /locus_tag="VF240_13810"
     CDS             6522..7286
                     /locus_tag="VF240_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176334.1"
                     /translation="MGNPIAGCPIGDKITNTIFEVKLQLSRRTMMGLESAILHFDHHP
                     KRGFRFERFYDGVKMWQASCQFEVRIELDTKFKGAERASIEYRQFICGGVWIRRGSEE
                     WTGDDKPNGNKHFLIPPYAGQTKSIGLPMAPVPGTGLSFQWKEDGEIEAGKIERYGYR
                     DTVPLVKTNEVDSWRPAHSLGHSYLLRDTPSIRGEWGGGDKVDVWIELFFQGFVVQVE
                     RDASDNTQPVRVLKQKSWQYLWQDQRLSEWLKATQI"
BASE COUNT         1301 a         2470 c         2312 g         1361 t
ORIGIN      
        1 gagacggatg tcctcgcggc ggagcgtggt gacgaagcgt cgctcgcggt caacggccgt
       61 caaaagtacg ctcgcgaggt caacgtcaac gcgctcgacc tcgtcgtccg gggcttcagt
      121 gggggagggc gtgggcgtgg gttgcgcggc gggaaccctc tgcgccccct ctgcgtcctc
      181 tgcgcctctg cggtgagctg ccgcggcctt aagctcaagc ggcgagtgca gcgccggctt
      241 cagatcaaac ggccccgccg tcgcgagcag cgccagcacg actacgaaca aagtgcgagc
      301 agacatccct ttccccttga cgattaacct gcgcgagagt tcttcagacg attcacgggg
      361 ccgcggtggg ggagttgttg ttgacgccga ccgaaggact caggcggacg gacgcgccct
      421 cgcgagccac gcgcacgcgc acgcgcgcgc ccgtcacggc cgccgcgccc gcgctcgact
      481 cataggtgat cgagtaggac gtgcgcagtt gcgcgaccac gtcgtcgtag gcgcgctgca
      541 actgttcgat acgcgtgatg gggtagtaga cgccgcccga gagttccgcc agccggcgcc
      601 cctcctcgcg tgcgcgcgag gtcagggtga gaaagcgcga gcgcatgggg tcgatggtgg
      661 acgccggggc cgccgcgctc gacgagggga tgaggcccga cggcacgtag agcggataga
      721 cgagcgcgcc ggattcgagc accgcttcga gcaattcgtt gaagggaatg aaggagcggt
      781 tgtcgtcgcc gtccgagagg atgacgacgg ccgtgcgctc gccgcggagc ggcttgaggg
      841 tatcgacgag cgtgtaggcc agcgcgtcat agagcgaggt ggcgccgccg gcctgaatgt
      901 ccttgatgcg ccgggcgagg gccgcgcggt cggtcgtgaa ctcggagatc acctgcacgt
      961 cgtcgcggaa ggaggtgatg gcgacacggt ctgtggggcc gaccgtttcg acgaaggcga
     1021 gcgccgcctt gcggatgaag tccagacgct cctcgacgct gcccgacacg tccaaaagca
     1081 atacgagatt gaagggcgcg gtcgatggct cgacggcgcg caccttgcgc tcctcgccgt
     1141 tttcgaccac ggtgaagtcg cgcgcggaga gaccgccgac ggcccgtccg ttgcggtcgg
     1201 tgacgctggc gtttaagcgc gcgagttcgg cgccgaggct cgttcgcacg tcgaaggggt
     1261 tgcggcgcgc ggcgaacgtc ggctcgaagc gcgtgggcgg cagtgtctcg gcgtactcgc
     1321 cgacgaggcg cgggacgagc ttgcggatgg cgtcggtgag gtctgcgtcg ccgctgcgga
     1381 tgacggcgcg cgccgcctcg gtcagctcgc gctcgcgcag gtcggagggc acggacgccg
     1441 cgtcggccac gccgaagagg atgacgccgc gcgccgtctc cagactcaac tcgatgcgcg
     1501 cgtccttttt cgccttctgg tctccgagcc ggccgctgat ctcgaaggcg ccggagcgtt
     1561 gttctttgac cttgccgagc gtgacctcgc tatagacgcg cggccgggcg agcgtccagc
     1621 gcaggctgta gcgcacggcg tccgtgggca cgtcggcgcg aatcgtgccc gtgcccgtcg
     1681 tcacacgggc ctcggccagt gggccgacca cgtccaccgc cccgacctcc gtctccacct
     1741 cgacacgggt acgcgccggc accgtcaaga ccacgtcgat gcggttgcga ttcgcctcgc
     1801 cgcccgcccc gacgcccgcg ctcgccttcg actctctctc cacgacgacc gacagacgag
     1861 cgcccgcctc ctcgatcttc acgtcccgct cttcgaccgt cactcccgag agcggggagc
     1921 gcgcgcgaat cgtgaccttc ttctcttcgc cctcgacggc cgtgaccgtg acgcgcccca
     1981 gacggttctt caccgagagg ttgatcggcc ccgacgccgc aagcacgcgc tcgaagccgc
     2041 cgtcttgcgc gcgcgcgcac acgcccgccg ccagcagcag cggaatcagg agaagaggtt
     2101 tgacgcgtat cttcatcctg ttcttttgtc gaggtaggcc gtgatggcgt cgcgcagttg
     2161 agtgatttgt tcctcggtga aggagccggc gacggcgcgg ccctcctgaa agccgaggag
     2221 catcacgcgg ccgtccatct ggaagttgaa ggtgtagttg acgccgttgt ctagctcgaa
     2281 gacgagtgcg ggcgacacgc tcgccgcgct tttcgattcc aggtgcatct cgggcagtct
     2341 ccgtccgtgg tggcttgtaa gtctgagaac cggagtcgtt agtttagcag agccgggcgg
     2401 tggggaacac ggataagacg gatgggacgg atgaacacgg atgtgatcgg tgctgatccg
     2461 ttagaatccg tttcatccgt gttccccacc gcgcaggccg caacgacgta ctgaaacctg
     2521 tcaatcacat tgacgacggg acatcccgcc cgtcgataaa ccgtgacggg gtcgaaatca
     2581 tctgatattt taacggcgta cacctcacct ttttcccgga ctttaacgga gcaccgaaag
     2641 attgaagaat aagattctga tttcgcttct cgtcgccctc gccctgtcgg ccgtcgccgc
     2701 gtttgcggcg ctgcccggcc gcggccagtc gcaggctgcc ggcgcgcagc aacagcaggc
     2761 gccttcgtcc gcacaggacg agcaggccgg cgccgctgcg tctcagaccg gcgtcaagct
     2821 cacggcctcc atccacgagc gacgccccgc cgctgcggtc gtgacgggcg acctctcggc
     2881 cttcgccgcc tccgcctcga agaacctgtc gctcaagtat gatttgacgt gggccttcgg
     2941 cgggaagcag cagcgcggct ggtatctcta cgcgtctctc atcggcagcc tcctcgacac
     3001 gaatgacgag accgggagcg ccggcttcgc cgccgccctc tcgcgctggc agcggcgcgc
     3061 cggtgtcgcg ccgaccggca tcctcgacga cgagacgctc taccgaatgg tctccgactg
     3121 gcagggcgcg cgcatcaaga gccgcgccta cccgcagccc tcggagctgg tcacggcgcc
     3181 gcccacggag ttctgggacc cctcgcggcc cgacgagctg cgacaggtgc gccgcgatgc
     3241 ctacgacgcc tacaagcgca tgctcgccgc ggccgtcgcc gacccttcgc tcaagctcgc
     3301 ggtgacgcgg acgggccagc tcgcgcccga ggagaagttc ctcaagatcg tctcctcctt
     3361 ccgctcgcgc gagtatcagg agcagttacg caagcgtcag cccaacgcgg gccgcgctgg
     3421 actggcggtc aacagcccgc acttcacggg ctgcgccctc gacctctacg tcggcggcga
     3481 ccccgtggac acgaaggacg ccaaccgcgc catacaggtg cagacgcccg tctaccagtg
     3541 gctcgtccgc aacgccggca agttcggctt ccgcccctac tactacgagc cgtggcactg
     3601 ggagtacgtg ggcgagtcga aggccggcgg cgttgagctt gtgggcgtga tgaagtaggg
     3661 tctgcggttt tctcaacagc ttgcagaaaa agggggcggc gcaggtcgat acctgcgccg
     3721 ccctctttat tcatcactcc ggccgagtcc gtctcggact cggccgccac ttctcaaaca
     3781 ctctctagct tccgcccatc acccgccacg gcttggggaa gatgcgccag ttgccgttga
     3841 cgtaggcgag gccgtcgtag gccatgccga ggtctttgcc gggttcgacg aacttgaagc
     3901 ggtagaactt cacgcccggc ttcagttgcg cgcccacgtc cttccagccg ccggggaact
     3961 ccgcggcgcc gcccgtccag ctcttcagct cgtcgctggt ggccgacgag gtcaagacct
     4021 gcgtctgccc ctccttcggg gcgaccacca tctgcccgcc gtcccacgcc gggccgtaga
     4081 cggtgtcggc cttggcggcc agatcgggca ggaagacggc gtcgtaatcc gccttcgacg
     4141 ggcgcagcgt cttgctcagt gtggcgtggt cggcgcccgg cttgacgaac tccgcgagca
     4201 gagccttcgc gccgtcatcc gtgccggggt gcttcgagga cgcgcccgcg cccgcgcccg
     4261 cgccgccgcc gccgcagccg gcgagcgcca gcgccagcgc cagcgcggcg atgagtcctg
     4321 atccgttaag cgtcttcctc ataaacgaca ttcctcctgt gaaatggttt tgactgagcg
     4381 cgctcccgga aactgcttca cgtccgaggc aggcccttca gaaattgcaa gatatcggtc
     4441 tgggccgact cgcgcaccat ggtattgcca tacgccttgc gctcctggct cggagtcttt
     4501 tcaaacaact tcttgtagac gaccgccgcc ttcgagcggt caaccatcgt cagctcgcaa
     4561 tcctcggcct cggccgggaa ctccttcccg tcgtcggtct tgtaggtgcc cttctgcgtc
     4621 gtcttgcagt gcatcagggc gaccgtcccc acttcctcgg gcgtcgcggc gtagaacgcc
     4681 tccaactccc ggaagtaatc gatctgcatg aaataagtgt tgtccctggc cgccgagcct
     4741 ttgctgcccc gcgcctgaaa gacggcgatc ttccctttga cgtatggctc ggcgacgagt
     4801 tccgtcttcg ccggcacggc cgtcaggtcg cccaccgccg ccatcatctt cttgttgatc
     4861 ggcccgaatt tttcttccgc cttgtatctt tcggagtcgc cgcaccccga aagacaggcc
     4921 gacatgaaca gggccgccgc ggccaaacgc actgatgttt tcatcgcttg cttcccgttc
     4981 gtcagatgtg tccgagcgcg cgccttatat caccggacat catctgacgc aaggccgagg
     5041 cgggcaacac gcgtcgcctc tcacacgcgc ccccgataaa ctaaaggcgg cgacatctct
     5101 gccgccgccc ctttcccgat catagttgag acccgcgggc taaaatcccg gccagccgcc
     5161 ctcgccgccg cccaaccgct gctcgtcaca ctgtcgccgc gccgaagaag tgcagaccgc
     5221 gtcgccgccg ccgttcaagc ccggcgtgtc cccgaagttc ccacccgcgc cgagttccgc
     5281 gcccgcgaat aacatgatcg acaacactat ccagaaagcc gtcatcgctt tttccccttt
     5341 cagacgggtg cgcgtctatc agccgcgcgc cctttttatc ctcacttgta aatgcgacac
     5401 cggctctcga aaacggtcac cccctctgga aataatttca gcggcttctt ttatctcgcc
     5461 cctgcgtccc gcttcagcgg ttttcaaaga ccccggcaca tctccaagcc ccagggagtt
     5521 tcgatggcgc gggcgagcgt caaccgcacc tcggcaaagt tccagtcctt gcgctgccag
     5581 atcgacccgt cggcatattc cacgcgattg atcaccacct gctcctggta aagattaccc
     5641 gtcttgtcag acaggcttcc cacgctgatc acagcgcccg gccccgaagg actgaaggcc
     5701 tgcaattctt tctccctggc gggcttgata ttgacgccgc aaagaaactg tcggcgcgcg
     5761 acattcgtcg gattcgccga ctccgtgaag cgatactccc agaacacgac ttcgatgact
     5821 ttcgcgctcg tgttgcggac tttgacccgg taggcaaagc cgtccacggc cttgcgtttg
     5881 ggagagcgcg cttcccgcac gttcctttcc agctccgcgc tacgactgtc aacggtgtcg
     5941 ccggtcggat tgctcacccc cgccggaaca ttagccaccc cggccgagga attggcccgc
     6001 cgtcctcgtt cgtaattttt attcgttgag atcatcgccg ccgccggggc ggtgctcgcc
     6061 gcttccgcga cctcggtcgt ctgacgactc ttcgaccact tgaaactggt cacgaccacc
     6121 gaagaaccgt tgtcagtgag cgcgccctcc tgacccggcg gaacgaacat gaagaggagc
     6181 ggcaagagta agagagttct catggtttgc ctccggcgat gaccgacgta gtctcgactg
     6241 tcaggctacg aagactcgcc cttcgtagaa agctggaagc gggggccgac cgtcttagct
     6301 acgccggctc aaatcgagga atgcgggctg cggcgacgga agcacctcgc ctcccgcctg
     6361 tttctgaatt ggaaagatag ttatcgacca cgccgctgtc aataattgac actgctcacc
     6421 tgcccggcgg gtgcgggcaa gcggtgaagc cccggaaacc aacgcgctcg tcgggtcggg
     6481 tgcaacgcct ggtcaggtaa cggtgcgtct cgacgctcgt agtgggcaat cccatcgccg
     6541 gatgtccaat cggtgataaa ataacgaaca cgatttttga ggtcaagctt caactctcaa
     6601 ggagaacgat gatggggctg gaatccgcaa tcctgcattt cgatcaccat cccaagcggg
     6661 ggttccgctt tgaacgattc tacgacggtg taaaaatgtg gcaggcttcc tgccagttcg
     6721 aggtcagaat cgaactcgac accaaattta agggcgcgga gcgggcttca atcgagtatc
     6781 ggcagttcat ttgcggcggc gtctggattc gccggggcag cgaagaatgg acgggggatg
     6841 acaaacccaa cgggaacaaa cactttctca tccctccgta tgccgggcag accaagtcga
     6901 ttggattacc gatggccccc gttcccggca cgggtctgag ctttcagtgg aaggaagacg
     6961 gcgaaatcga ggccggaaag atcgaacgct acggctatcg cgacacggtc ccgttggtca
     7021 aaaccaacga agtagacagc tggaggccgg cacactcgtt gggtcacagc tatttgctgc
     7081 gcgacacccc gtcgatccga ggggaatggg gaggcgggga taaggtcgat gtctggatcg
     7141 agttattctt tcagggtttc gtggtgcagg ttgagcgtga cgcgagcgac aatacacagc
     7201 ccgtccgggt cctcaaacaa aaaagctggc agtacttatg gcaagaccag aggttgtcgg
     7261 agtggcttaa ggccacgcaa atctaaccgc tcaacccccg gtcagccttt cgccgactaa
     7321 cgctccagat aacccgcgag catcaaacca gacctttaga aggcgcgctg ggggcgagtc
     7381 gggttcatct ggttgttatg cgcggcttgc cagggatatc gtcagaagcg ttggtctgcc
     7441 accc
//
LOCUS       DASVES010000338        12383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1571781, whole genome shotgun sequence.
ACCESSION   DASVES010000338 DASVES010000000
VERSION     DASVES010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12383
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571781"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF240_13815"
     CDS             complement(<1..291)
                     /locus_tag="VF240_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176335.1"
                     /translation="MFTPTIHFRTASRLALALLATLLLAALPFPHAAQKADPPGQQPQ
                     PSQTPPSQPMGGVSTGTALVSATRRTVGVVDPKAPKVFEDVTAQTGLAAFRHR"
     gene            complement(399..2198)
                     /locus_tag="VF240_13820"
     CDS             complement(399..2198)
                     /locus_tag="VF240_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX7176336.1"
                     /translation="MSFSRKPCALLFAATAAHLLAFLFAPLLPHAQTPSPSPSATPAR
                     TGRSYETGAVPSRRPQPSAPQQPSPVTFTDVTAQSGIRFRHAASPTSQKYLPETMGGG
                     VALLDFDADGRLDIYFTNGAALSDPMPRGAAPDKRDPRFWNRLYRQKPDGTFEDVTER
                     SGLRGEGYSMGAAAADYDGDGFVDLYVTSYGGARLYRNRGDSTFEDVTARTNTNAPGW
                     PTSAAWLDYDADGRLDLFVARYMEWDFDRGSIFCGEPRTRAYCHPDNFKGASSLLLRQ
                     KPDGAFEDASAKAGIASSEGKGLGVAVADFDNDGLSDVFVANDGVRQLLFRNSGGGRF
                     EEIALPAGVGYDENGKTFAGMGVDAADYDGDGLVDVFITALSNETYPLFRNNGDQSFT
                     YTTQTAGVGPATLTYSGWGARFADVDNDGWRDLVVAQGHVLDTIEKTSGYLTYKQPPL
                     LLRNTGKNFVNVSATAGPAFSMPLAARGAAFGDLDSDGRLDLVFGQTDGAPIILRNGG
                     TRNHWLGVVLVGAKGNRHGLGARVAVTDSAGRRQIFDVTTAGSYLSSNDPRLIVGLGS
                     ASSVRSVEVRWPSGKSQTVESPAIDKYLTITEK"
     gene            2373..2894
                     /locus_tag="VF240_13825"
     CDS             2373..2894
                     /locus_tag="VF240_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176337.1"
                     /translation="MKKLGTWRVLASVAVIALCAAGVSAQEEQQQAVILAGQQLARVV
                     PPGFYFEGQSAPTQTRNSAAARFGAKRHVIAGMVDTSGYSADVRAKYEGFFITDSPVS
                     VGGEQLGPGAYGFGFTDDGKFNLFDVGGRPLLSVGAAADRVIRRPRPLMMTKADGGVR
                     LYSGRQFVVITAR"
     gene            2954..3400
                     /locus_tag="VF240_13830"
     CDS             2954..3400
                     /locus_tag="VF240_13830"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7176338.1"
                     /translation="MAKSDAATVEEYLEGLPEERREVVSRVREVILSNLPRGYRESMN
                     WGMIAYEVPLEKYPDTYNKQPLGYAALGAQKNYISLHLMCAYQDSELEKKLKEGFAKA
                     GKKLDMGKSCIRFKKLEDIPLEVIGEAVASTPPEKFIEQYEKGRHK"
     gene            3454..4455
                     /locus_tag="VF240_13835"
     CDS             3454..4455
                     /locus_tag="VF240_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7176339.1"
                     /translation="MEHLRYSVAACQTDLPNPVERREMRRNTDRCLSMIDSAVAGSAP
                     FLPVRLIVFPEFAHAAPVFPTVAELHEKLAVQIPNEHTEALEQKAREHRVYIQTGSML
                     ERDARWPGRVFNTTCLIGPRGVLYKYRKVNTWIPYEVHSSPHDLEGYDEPLFPVADTE
                     IGRLGCAICYDWLFPEAIRQLAANGAEVLIRVSAYMDPWGATAPMDWWTIINRARAIE
                     NTAYVVAANQGASLKHYPPYSWPGGSQVVDFDGRLLAEASPGPGERIVVAPLDISALR
                     HERAARRGHHMLAHLRTEAYPVYATHRYPPVETGQDEQGLSYEGNNRLIDEAKNRLP"
     gene            4860..8285
                     /locus_tag="VF240_13840"
     CDS             4860..8285
                     /locus_tag="VF240_13840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7176340.1"
                     /translation="MYTFRSGFSSSMRGAAALLFVIALSLSAHAQSSKGEISGSVTDA
                     NGAAVPKVNVKATNNGTGAVREDTASDDGAYSLQLLDPGLYTVEATAPNFQTYRAENV
                     KVDVGSTQALPITLQAGQITDVVTVTAEAPLVQSESSDRGSVISGREVTELPLSGRNF
                     TQLALLTPGVARAANAGVGGAGPDSRQFNNGDPRAGSGGPGSSNAQGDTPTSRFARSG
                     GGALTVNGQRPTNNNFSLDGVDNNESQFGTVGVFPNPDAIAEFRVTTSVPPAEVGRAS
                     GAVINTTTKSGRNDFFGSLYYYGQNSALNAYHPMLKRDRAIAQSRGDTFIPEKAVQQI
                     HEFGGTFGGRIVRDRTFFFFDYLGQRNNLPFPFTSTVPALGVTSGDFSAYSTIRDPLT
                     GLPFPNNRIPANRLDPIAVRILDLFPDPTANIFNPGEFNRNYASQRNVQERINNYGIK
                     VDHRFNSSNSLTVRFNDQKLKNVRDNFFKTPDDVPAPGLPTAGFGAGEEIGDTRQLVI
                     TDTHTFSPTLLNEFRFGLTNINIAIYNCGVLGACGVSETWGTDLGIPNVNLGGPETSG
                     GPGIGWTGAGFVEFLGDGGLFQAKSRNPYFADSLTILKGNHVVKTGAEIRLRMLETIC
                     GGCAGFEKGAIGFDDGGTGNALANSLLGRTGFAQRSRTFGGPFDLRMQEYGFFVQDDW
                     KVNDRLTLNLGMRYDLLPGPNAATETGGRIGYYFPEDRRIVEASGSGDRIVELDRNNF
                     GPRVGFAYAVNDSKTLVVRGGYGLLYSLDGVDYPAGVRNPPNTNITFDNWTLASGPPT
                     PTPLDPANVPTGTNLFTVDRRQKNGMTHQFQLSTQWQFSPDWSLDVGYVGNRSKNLLA
                     TLQQGSGGQGVARNAAGALINSVLLYSNVAESSYDALQTQLQKRLSNNIQGQVSYTWS
                     HTIDNATGVFNGLGDAKNQGRQGPVDPFNLDADRGNSVLDIRHLLSASAIIDLPFGKG
                     QRYLNDGMGARILGGWQLNFIQSARSGFPFTVTCNCGLIRPSLVGDPFANVPFGRALN
                     PAAFSTTVGITNVTNAAGQTVSYGSLGRNTFRGPAIWNTDMSLFKNTSITEKLKFQLG
                     IEAFNLWNHTNLTVPNNNSSDVGNDRGRPTGFGVFDGAYPGRFLQYRLKFIWN"
     gene            8466..10031
                     /locus_tag="VF240_13845"
     CDS             8466..10031
                     /locus_tag="VF240_13845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176341.1"
                     /translation="MLCRPLLILTIAPVFAAAATTTARAQAGTLTPAAPPRSHVERFS
                     TVEMRAERVEADANSKLTADPRDAAALNSRAVARLFLGRYREAHEDLRRAVSIKPDRA
                     AYHANLGSALWKLGRADEAVAAERAAVRLDDKNFQARYQLGRFLLRLGGRERTTEAVE
                     HLRRALEIDPRQHDVRLELITAYRALGDRASASNHLDFLWDARPSDPRVFYTSALLAT
                     DRDDLTAAIKDFREALRRDPAFHSAWQDLGVAYMKLKRWDEAIETFTELARLRPESVE
                     AAYLHALSLFNAGRAREAEGEARRALRINAGAAEAHTLLGVVLASRGDASAEAVETLG
                     QAAALSPNSFDAHFYLGRVLYSMRDYASAVKDLSAAVRLNPRHAEARFFLGTALEASG
                     DSRAALEQYQELVKIDPQSVYGQLGTGALLVKQGKTEEAVAALARASASDPKNFEAHL
                     ALGRAHALAGRHAEAVASLERAAALAPHRPDARYQLGLSLRRLGRDEDAKREFAIVEK
                     LNADFRAGVTTRH"
     gene            10300..12018
                     /locus_tag="VF240_13850"
     CDS             10300..12018
                     /locus_tag="VF240_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176342.1"
                     /translation="MKTFSELSRVPARDGRPSPALVLSLRLITASLAFVFALQLASSR
                     AHAQPASQRGRPNAVQRGGASTSELSAAARSSLDAAVEALQRDALADAERAARVAVGA
                     APRSPVARNVLGVVLDRLGRRDEAVAEFDAAIRLDPDFVGARNNLARTLAERGRTAEA
                     VAEFERVLKVDPTHAQARFNLGSLHAATGDFAKAAEHFARARATSPDDARLALAFLSV
                     AYRAGRKAEADAAADTVERAALSDARALFTLATVLAQSNQYERAARLYARVNELTPRT
                     YEVLYNLGVALYNLERGEEAARHLAEAADLNPDPAETHFRLGLIASERGDSANAVEEL
                     RHAVERDPKSANYRHLLGREYFRAGFWEGAIAEFTRAAEIEPAQAQHYLSRADANYRK
                     GEAAAAAADFDRAAELDPKIENIGYWQGYTHRVAGDFEVARRRLEKFVAAHPAHADAL
                     ASLGFIAIEQGRLEEAEQPLRRALELDAKNVPALYDYARVALKRRDYAEAVSRFEHVV
                     EKHPAYTQAYYQLFLAHTRLKQTEKAQAALAEFKRLEGLEKQTTQERMADDKVRTQQM
                     LGQSPR"
     gene            12169..>12383
                     /locus_tag="VF240_13855"
     CDS             12169..>12383
                     /locus_tag="VF240_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176343.1"
                     /translation="MRRTTFVALAAVCLAAIATRAQEQQSWSKPLEEWTREDAQKVLN
                     ASPWARRQSIKVEQRRTGRIAGAPNPI"
BASE COUNT         2200 a         4313 c         3930 g         1940 t
ORIGIN      
        1 acggtggcgg aaggcggcaa ggcccgtctg cgccgtcacg tcctcgaaga ccttcggagc
       61 cttcgggtca acgacgccga ccgtgcgccg cgtggccgag acgagcgccg tgcccgtcga
      121 gacgccgccc atcggctgcg atggtggcgt ttgcgacggc tgcggttgct ggccgggcgg
      181 gtcggccttc tgcgcggcgt gtgggaaggg aagggccgcg aggaggaggg tcgccaagag
      241 ggccaaggcg agtcgagacg ctgtgcgaaa gtgaatggtc ggtgtgaaca tagtcggctt
      301 tgactcgtcg gttctgaatt gatgctgact ctactcttga gtgagtgcga agggcggctc
      361 accgcagagg cgcagagggc gcagagaata cgcagaggct acttttccgt aatcgtcaga
      421 tacttgtcaa tcgccggact ctcaaccgtc tgactcttgc ccgacggcca gcggacttcg
      481 accgagcgca cgctcgacgc agagccgagg ccgacgatga ggcgggggtc gttcgacgag
      541 aggtaactgc cggcggtcgt cacgtcgaag atttggcggc ggccggcgga gtcagtgacg
      601 gcgacgcgag cgccgaggcc gtggcggttg cccttcgcgc cgacgagcac gacgccgagc
      661 cagtggttac gtgtgccgcc gttgcgaaga atgatgggcg ctccgtccgt ctggccgaag
      721 acgaggtcaa gacggccgtc cgagtcgagg tcgccgaagg cggcgccgcg ggcggcgagg
      781 ggcatggaga aggcggggcc ggcggtggcc gagacgttga cgaaattctt gcccgtgttt
      841 cgcagcagca gcggcggctg tttgtacgtg agatagccgg aggtcttttc gatggtgtcg
      901 aggacgtggc cctgcgcgac gacgaggtcg cgccagccgt cgttgtccac atcggcgaag
      961 cgcgcgcccc agcccgagta ggtgagcgtg gcggggccga cgccggccgt ctgcgtggtg
     1021 taggtgaacg actggtcgcc gttgttgcgg aagagcggat aggtttcgtt cgagagggcg
     1081 gtgatgaaca cgtccacgag gccgtcgccg tcgtagtcgg ccgcgtccac gcccatgccg
     1141 gcgaaggtct tcccgttctc gtcgtagccg acgccggccg ggagcgcgat ctcctcgaag
     1201 cgtccgccgc ccgagttgcg gaagagtaac tggcggacgc cgtcgttggc gacgaacacg
     1261 tcggagagtc cgtcgttgtc gaaatcggcg acggccacgc cgaggccctt gccctccgaa
     1321 gaggcgatgc cggccttcgc gctggcgtcc tcgaaagcgc cgtccggctt ctgccggagc
     1381 agaaggctcg acgcgccctt gaaattgtct gggtgacagt aggcgcgtgt gcgcggctcg
     1441 ccgcagaaga tggagccgcg gtcgaagtcc cattccatgt agcgcgcgac gaagaggtcg
     1501 aggcggccgt cggcgtcgta gtcgagccag gcggcgctcg tcggccagcc cggcgcgttc
     1561 gtgttcgtgc gcgccgtcac gtcttcaaac gtggagtcgc cgcggttgcg gtagagacgc
     1621 gcgccgccgt aggaagtgac gtagagatcg acgaagccgt cgccgtcgta gtcggcggcc
     1681 gccgcaccca tcgagtagcc ctcgccgcga agacctgagc gctcggtcac gtcttcaaac
     1741 gtgccgtcag gcttctgtcg gtagagtcgg ttccagaaac gggggtctcg cttgtcgggg
     1801 gcggcgcctc tgggcatcgg gtcggagagg gccgcgccgt tggtgaaata gatgtcgagg
     1861 cggccgtccg cgtcgaagtc gaggagggcg actccgccgc ccatcgtttc gggcaggtac
     1921 ttctgcgagg tgggcgaggc ggcgtggcgg aagcgtatgc cggactgcgc ggtcacgtcc
     1981 gtgaaggtga cgggcgacgg ctgctgcggt gctgacggct gaggccgtct tgacggcact
     2041 gcgccggtct cgtaggagcg gccggtgcgc gcgggcgtgg ccgagggaga cggcgaggga
     2101 gtttgtgcgt gggggagcag gggagcgaag aggaacgcga gcaggtgagc ggcggttgcg
     2161 gcgaagagga gcgcgcaagg ttttcggctg aagctcatta caggcaaatc gggccagtgg
     2221 gggagttcgg aatgcgacgc ctcgcggagc gtggacgcgt gcgttttgtg actccgactt
     2281 tacagtcgga agtcccgaat agtacagtct cgccgccgca ccggcaactg catcgggcgg
     2341 gcaacgctaa agtcgcagtc atgggaggac gcatgaaaaa attaggaaca tggagagtgt
     2401 tggcgagcgt cgccgtaatc gccttgtgtg ctgcgggcgt gtcggcgcag gaggagcagc
     2461 agcaggcggt catcctcgcc gggcagcagc tcgcgcgcgt cgtcccgccg gggttctact
     2521 tcgaggggca gagtgcgccg acgcagacgc gcaactcggc cgcggcccgc ttcggcgcga
     2581 agcgtcacgt catcgccggg atggttgaca cctccggcta ctcggccgac gtgcgcgcca
     2641 agtatgaagg gttcttcatc accgactcgc ccgtcagcgt cggcggcgag caactcggcc
     2701 ccggcgccta cggcttcggc ttcaccgacg acggcaagtt caatctcttc gacgtgggcg
     2761 gccgacctct actctccgtc ggggccgcgg ccgaccgcgt tatccgccgg ccgcgcccgc
     2821 tgatgatgac gaaggccgac ggcggcgtgc gcctctacag cggccgtcag ttcgtcgtca
     2881 tcacggcgcg ttgaaggaca aggacatcgg aaggtgcgag gtgattacct gagagccggg
     2941 aagggaattc aacatggcga agagtgatgc ggcgacggtc gaagagtatc tggagggtct
     3001 gccggaagag cggcgcgaag tcgtctcgcg cgtgagggaa gtgattctga gtaatctccc
     3061 gcgcggctac cgcgagtcga tgaactgggg gatgatcgcc tacgaggtgc cgctggagaa
     3121 gtaccccgac acctacaaca aacagcctct cggctacgcc gcactcggtg cgcagaagaa
     3181 ctacatctcg ctccatttga tgtgcgccta tcaggactcc gagttggaga agaagctgaa
     3241 agagggcttc gcaaaggcgg ggaagaaact cgacatgggc aagtcgtgca tccgcttcaa
     3301 gaagctcgaa gacatcccgc tggaagtcat cggcgaggcc gtcgccagca caccgcccga
     3361 gaagtttatc gagcagtacg agaaggggcg gcataagtag acgcagcggg agtagcctgc
     3421 tggcgcggcg aaatataaca agggggacga atcatggaac acctgcgtta ctcggttgcc
     3481 gcgtgccaga ccgatttgcc gaacccggtc gagcgccgcg agatgcgtcg caacaccgac
     3541 cgctgtctct cgatgatcga ctcggccgtc gccggctccg cgcccttcct gcccgtccgc
     3601 ctcatcgtct ttcccgagtt cgcgcacgcc gccccggtct tcccaacggt cgccgagctg
     3661 cacgagaagc tagccgtcca aatcccgaac gagcacaccg aagccttgga gcagaaggcg
     3721 cgcgagcacc gcgtctacat ccagaccggc tcgatgctgg agcgcgacgc gcgctggccg
     3781 gggcgggtct ttaacaccac ctgcctcatc ggcccccgcg gcgtcctcta caagtaccgc
     3841 aaggtgaaca cgtggatacc ctacgaggtg cacagcagcc cgcacgacct cgaaggctac
     3901 gacgagccgc tgttccccgt cgccgacacc gagatcggtc gattgggttg cgccatctgc
     3961 tacgactggc tcttccccga agccatccgc cagctcgccg caaacggcgc cgaggtgctc
     4021 atccgtgtct cggcctacat ggacccgtgg ggcgccaccg cccccatgga ctggtggacg
     4081 atcatcaacc gcgcgcgggc catcgagaac acggcctacg tcgtcgccgc caatcagggc
     4141 gcaagcctca agcactatcc gccatattca tggcccggcg gcagtcaggt cgtcgatttc
     4201 gacggacgcc tcctcgccga ggccagtccg gggccgggcg agagaatcgt cgtcgcgccc
     4261 ttggacatct ccgcactgcg acacgagcgc gccgcccggc gcggtcatca catgctcgca
     4321 cacctccgca cggaggctta ccccgtctac gccacccacc gctatccgcc ggtggagacg
     4381 ggccaggatg agcaaggtct atcctacgag ggcaacaacc gcctcatcga cgaggcgaag
     4441 aaccgacttc cctgattgtt gacgctacgc ccacgcaggc gaacacagat gaaacggatg
     4501 cgacggatga acacggattc atgctcatcc gtctcatccg tcgcatccgt gttcccgccc
     4561 gcacgcgctc aggcgcaccg tcccaactaa tcgaacgaat cgacatcgaa gtacgcgctg
     4621 ccgccctgcg gcttgaaccc gccccaaata aagattgaca ggcggttgaa tctgtaataa
     4681 aaacgacttc gttcgggcgt gcaacaaacc tccctgcgaa tgcggttttc aagttacaag
     4741 ttttcttcag ggacagggct acatctccac ccatcgattc gtcgccggta acgcaccgtg
     4801 cgacggtcat cgctacgggt ggcgcaatca tccggctcac tccacctgcg aggttcgcta
     4861 tgtatacatt ccgaagtggc ttctcctcct ccatgcgcgg agccgcggct ctcctcttcg
     4921 ttatcgcctt atctctttcg gcccacgcgc agtccagcaa gggcgagatt tcgggcagcg
     4981 tcactgacgc caacggagcc gccgtcccca aggtgaacgt gaaggccact aacaacggga
     5041 ctggcgccgt gcgcgaggac acggcgagcg atgacggcgc gtacagcctc cagctcctcg
     5101 accccggcct ctacaccgtc gaggccacgg cgccgaactt ccagacctat cgcgccgaga
     5161 acgtgaaggt ggatgtcggc tccacgcagg cgctcccgat cacccttcag gccggacaga
     5221 tcaccgacgt ggtgacggtg acggccgaag cgccgctcgt gcagagcgag tcgagcgacc
     5281 gcggcagcgt catctcgggc cgcgaggtca cggagcttcc gctctcggga cgcaacttca
     5341 cgcaactggc gttgctcacg ccgggcgtgg cgcgcgccgc caacgccggt gtcggcggcg
     5401 ccgggcctga ctcgcgccag ttcaacaatg gcgacccgcg cgccggctcg ggcggccccg
     5461 gcagctccaa cgcgcagggc gacacgccga cctcgcgctt cgcacgctcc ggcggcggcg
     5521 ccctgaccgt caacggccag cggcccacca acaacaattt ctctctcgac ggcgtggaca
     5581 acaacgagtc gcagttcggc accgtcggcg tcttcccgaa cccggacgcc atcgccgagt
     5641 tccgcgtcac gacgagcgtg ccgccggccg aggtcggccg cgccagcggc gccgtcatca
     5701 acaccacgac caagtcgggc cgcaacgact tcttcggctc gctctactac tacggccaga
     5761 actcggccct gaacgcctat cacccgatgc tcaagcgcga ccgcgccatc gcccagtcgc
     5821 gcggcgacac cttcatcccc gagaaggccg tccagcagat acacgagttc ggcggcacct
     5881 tcggcggccg catcgtccgc gaccgcacgt tcttcttctt cgattacctc ggccagcgca
     5941 acaacctgcc gttccccttc acctcgacgg tgccggccct cggtgtgacc agcggcgact
     6001 tctcggcgta ctcgaccatc cgcgacccgc ttacggggct gccgttcccc aacaaccgca
     6061 tcccggccaa ccggctcgac cccatcgccg tcagaatcct cgacctcttc cccgacccga
     6121 cggccaacat cttcaacccc ggcgagttca accgtaacta cgcctcgcag cgcaacgtgc
     6181 aggagcgcat caacaactac ggcatcaagg tcgatcatcg cttcaactcg tcgaactcgc
     6241 tgaccgtgcg cttcaacgac cagaagctga agaacgtgcg cgacaacttc ttcaagacgc
     6301 ccgacgacgt gcccgcgccg ggtctgccga cggccggctt cggcgccggc gaggagatcg
     6361 gcgacacgcg ccagctcgtc atcaccgaca cgcacacctt cagcccgacc ctcctcaacg
     6421 agttccgctt cggcctgacg aacatcaaca tcgccatcta caactgtggt gtgctgggcg
     6481 cctgcggcgt cagtgaaacc tggggcacag acctcggcat cccgaacgtc aacctcggcg
     6541 gccccgagac ctcgggcggc cccggcatcg gctggacggg cgcgggcttc gtcgagttcc
     6601 tcggcgacgg cggcctcttc caggccaaga gccgcaaccc ctacttcgcc gacagcctga
     6661 ccatcctcaa gggcaaccac gtcgtgaaga cgggcgccga gattcgcctc cgcatgctcg
     6721 agacgatctg cggcggttgc gccggcttcg agaagggcgc tatcggtttc gacgacggcg
     6781 gcacgggcaa cgccctcgcc aactcgctcc tcggccgcac cggcttcgcc cagcgcagcc
     6841 gcaccttcgg cggccccttc gacctccgca tgcaggagta cggcttcttc gttcaggacg
     6901 actggaaggt caacgaccgt ctgaccctca acctcggcat gcgctacgac ctcctccccg
     6961 gccccaacgc cgccaccgag accggcggcc gcatcggcta ctatttcccc gaagaccgac
     7021 gcatcgtcga ggccagtggt tcgggcgacc gcatcgtcga actcgaccgc aacaacttcg
     7081 gcccgcgcgt cggcttcgct tacgccgtca acgactcgaa gactctggtc gtgcgcggcg
     7141 gctacggcct gctctactcg ctcgacggcg tggactaccc cgcgggcgtg cgcaacccgc
     7201 ccaacaccaa catcaccttc gacaactgga cgctcgcctc ggggccgccg acgccgacgc
     7261 cgctcgaccc ggccaacgtg cccacgggga cgaacctctt caccgttgac cgccggcaaa
     7321 agaacgggat gacgcaccag ttccagttga gcacccagtg gcagttctcg cccgactggt
     7381 cgctcgacgt gggctacgtc ggcaaccgct ccaagaacct gctcgccacg ctccagcaag
     7441 gctcgggcgg acagggtgtc gcgcgcaacg ccgccggcgc tctcatcaac agcgtcctgc
     7501 tctactcgaa cgtcgccgag tcgtcctacg acgccttgca gacgcagttg cagaagcgtc
     7561 tgtcgaacaa cattcagggg caggtctcct acacgtggag ccacaccatc gacaacgcga
     7621 ccggcgtctt caacggcctc ggcgacgcca agaatcaggg ccggcagggg ccggtcgatc
     7681 cgttcaacct cgacgccgac cgcggcaact ccgtcttgga catccgccac ctgctctcgg
     7741 ccagcgcgat catcgacctg cccttcggca aaggacagcg ttatctgaac gacggcatgg
     7801 gcgcgcgcat cctcggcggc tggcagttga acttcatcca gtcggcgcgc tccggcttcc
     7861 cgttcaccgt cacctgcaac tgtggactca tccggccgtc gctcgtcggc gacccgttcg
     7921 cgaacgtccc cttcggacgc gccctgaacc ccgcggcttt ctcgaccaca gtgggcatca
     7981 ccaacgtgac gaacgccgcc ggccagaccg tcagctacgg cagcctcggg cgcaacacct
     8041 tccgcggccc ggcgatctgg aacaccgaca tgtcgctctt caagaacacg tcgattaccg
     8101 agaagttgaa gttccaactc ggcatcgagg ccttcaacct ctggaaccac acgaacctga
     8161 cggtgccgaa caacaactcg tcggacgtgg ggaacgaccg cgggcggccc accggctttg
     8221 gcgtcttcga cggcgcctat cccggccgct tcctccagta ccgtctgaag ttcatctgga
     8281 actaggcggg gccccgaggg ggccgcgaca gtcggcaggc ccgaatgcga atcagaagcc
     8341 cggagcggcg gtgagttttt ggccgccgct ccggtttgtt ttagaaggcg ctcggctcgc
     8401 accgagccgc cgctgtctga tagaatcgcc cgcgtccgac gagccgcccc gcccgtgcag
     8461 ttcttatgct ttgtcgcccg ctccttatcc tgacgattgc tccggtcttc gccgccgctg
     8521 cgactacaac ggcgcgcgcg caggccggca cgctcacgcc tgctgcgccg ccgcgctcgc
     8581 acgtcgaacg cttcagcacc gtcgagatgc gcgccgagcg ggttgaagcg gacgccaact
     8641 caaagcttac cgccgacccg cgggacgccg ccgcgctcaa ctcccgcgcc gtcgctcgcc
     8701 tcttcctcgg ccgctaccga gaggcccacg aagaccttcg ccgcgccgtc tcaatcaagc
     8761 ctgaccgggc cgcctatcac gccaacctcg gctccgctct ctggaagctc ggccgcgccg
     8821 acgaggccgt cgccgccgag cgcgccgccg tccgtctgga cgataaaaac tttcaggcgc
     8881 gctaccagct tggccgcttc ctcctgcggc tcggcgggcg cgagcgcacg accgaagcgg
     8941 tcgaacacct ccggcgcgcg ctggagatcg acccgcgcca gcacgacgtt cgccttgaat
     9001 tgatcaccgc ctatcgcgcg ctcggcgacc gcgcttcggc ttccaatcac ctcgacttcc
     9061 tctgggacgc gcgcccttca gacccgcgcg tcttctacac gagcgcgctc ctcgccaccg
     9121 accgcgacga cctcacggcg gccatcaaag acttccgcga ggccctgcgc cgcgaccccg
     9181 ctttccacag cgcgtggcag gatttgggcg tcgcttacat gaagctcaag cgttgggacg
     9241 aggccatcga aaccttcacg gagcttgcgc gcctgcggcc cgaatcggtc gaggccgcct
     9301 acctccacgc cctctcgctc ttcaacgcgg gccgcgcgcg tgaggccgaa ggggaggccc
     9361 gccgcgccct gcggatcaac gccggcgctg ccgaggccca tacccttctt ggcgtcgtcc
     9421 tcgcttcgcg cggcgacgcg agcgccgagg ccgtcgagac gcttgggcag gccgccgcgc
     9481 tcagccccaa cagcttcgac gcacacttct acctcgggcg cgtgctctac tcgatgcgcg
     9541 attacgccag cgcggtaaaa gatttgagcg ccgccgtccg actcaacccg cgccacgccg
     9601 aggcgcgctt cttcctcggc acggcgctcg aagcgtcggg cgactcgcgg gccgcgctcg
     9661 aacagtatca ggagctggtg aagatcgatc cccaatcggt ctacgggcaa ctcggcacgg
     9721 gcgccctgct cgtcaaacag ggaaagacgg aggaggccgt ggcggcgctc gctcgcgcgt
     9781 ctgcgtccga cccgaagaac ttcgaggccc acctcgcgct cgggcgcgcg cacgcgctcg
     9841 cgggccgcca cgccgaagcc gtcgcttctc tcgaacgcgc cgccgcactc gcgccgcacc
     9901 gcccggacgc acgttatcag ctcggactct cgctccgccg gctcgggcgc gacgaagacg
     9961 ccaaaaggga attcgccatc gtggaaaaac taaacgcaga cttccgcgcg ggcgtgacga
    10021 ccagacatta acagcgttgc aagattgtag gggcagggca agtccgcggc ccgcgactct
    10081 caagacattc aaacatggat ggacaggaca aacaggataa gaagatttga aatctccaat
    10141 ctgaaatctc aaatcagcgc tttcttcatc ctacctatcc tgtccatcca tgttaattct
    10201 gctgttgaca cgggccgcgg acaagctctg ctcctacaga catcgaccgc gttgaggttt
    10261 gaacgcagac gctaagctga gagggctgag gtcagggaag tgaagacgtt ttcggagttg
    10321 tcgagagttc cggcgcgcga cgggcgacca agccccgcgc tcgttctctc actccgtctc
    10381 atcacggctt cgctcgcgtt cgtcttcgct ttgcaactcg ccagttctcg tgcccacgca
    10441 caacccgcca gtcaaagagg tcggcccaat gcggtgcagc gcggcggagc gtccacttca
    10501 gagttgagcg ccgcggcgcg ctcctctctc gacgccgccg tcgaagccct ccaacgtgat
    10561 gctctggccg acgccgagcg cgcggcccgt gtggccgtcg gggccgcccc gcgctcgccc
    10621 gtcgcccgta atgtcctcgg cgtcgtgctc gaccgcctcg gccgacgcga cgaggccgtc
    10681 gccgagttcg acgcggccat ccgcctcgac ccggatttcg tgggcgcgcg caacaacctc
    10741 gcgcgcactc tggccgagcg cggccgcacc gccgaggccg tcgccgagtt cgagcgcgtg
    10801 ctcaaggtag acccgacgca cgcgcaggcc cgattcaacc tcggctcgct ccacgccgcc
    10861 acaggcgact tcgccaaagc ggccgagcac ttcgcccgcg cgcgcgccac ttcccccgac
    10921 gacgcgcgcc tcgcgctggc cttcctgagc gtcgcctatc gtgccggccg caaggccgag
    10981 gccgacgcgg cggccgacac cgtcgagcgc gcggccctct cagacgcacg cgccctcttc
    11041 acgctcgcca ccgtgctcgc gcagagcaat cagtacgagc gtgccgcgcg tctctacgca
    11101 cgcgtcaacg agctgacgcc gcgcacctac gaggtgctct acaatctcgg cgtcgcgctc
    11161 tataatctcg aacgcggcga ggaagcggcc cgacatttag ccgaggccgc cgacctcaac
    11221 cccgaccccg ccgagacgca cttccgcctc ggcctcatcg ccagcgaacg cggcgacagc
    11281 gccaacgccg tcgaagagct gcgccacgcc gtcgagcgcg acccgaagag cgccaactac
    11341 cgacacctgc tcggccgcga gtacttccgc gccggcttct gggagggcgc gattgcggag
    11401 ttcacacgcg ccgccgagat cgagccggct caggcgcagc actatctttc gcgggccgac
    11461 gccaactacc gcaagggcga ggcggcggcc gctgccgcag acttcgaccg cgccgccgaa
    11521 ctcgacccga agatcgagaa catcggctac tggcagggct acacgcaccg cgtcgccggc
    11581 gacttcgagg tggctcgccg gcgcctcgaa aagttcgtcg cggcgcaccc ggcgcacgcc
    11641 gacgccctcg ccagcctcgg cttcatcgct atcgagcagg gtcggctcga agaggccgag
    11701 cagccgctcc ggcgcgcgct cgaactcgat gcaaagaacg tccccgcgct ttacgactac
    11761 gcgcgcgtcg ccctcaagcg gcgcgactac gcggaggccg tttcgcgctt cgagcacgtc
    11821 gtcgagaagc acccggccta cacgcaggcg tactatcaac tgttcctcgc gcacacgcgt
    11881 ctcaagcaga cggagaaggc gcaggccgcg ctcgccgagt tcaaacggct ggagggtctg
    11941 gagaagcaga ccacgcagga gcgcatggcc gacgacaagg tgcggacgca gcagatgctc
    12001 ggccagtcgc cgcgctaaga cggccgacgt acttcgcggc cgtgcgtcgc aatcgaagcc
    12061 gccgcgaggg ccgcacgccg gaaggcgtcc gagggtttat acaagggaag ttttcacaaa
    12121 agggaagtag taatgagcat gagttttacc ggaagtgatt cgagaagaat gcgccgcacg
    12181 acgttcgtgg ccctggcggc ggtgtgtctc gctgccatcg cgacgcgcgc gcaggaacag
    12241 cagtcgtggt cgaagccgct tgaggagtgg acgcgggagg acgcgcagaa agttttgaac
    12301 gcttcaccgt gggcgcgcag acagtcgatc aaagttgagc agcgcaggac cggccgcatc
    12361 gccggcgcgc cgaacccgat cat
//
LOCUS       DASVES010000339         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1196873, whole genome shotgun sequence.
ACCESSION   DASVES010000339 DASVES010000000
VERSION     DASVES010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196873"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..1156)
                     /locus_tag="VF240_13860"
     CDS             complement(353..1156)
                     /locus_tag="VF240_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7176344.1"
                     /translation="MEEKITSRHNPLVRRAREVREGKDRGLVFVEGLRLCEEAAHAGL
                     EVAAVLHTDTFARDERAQRLLDSLSTAAAKVVAVSDAVMGQLSDTKSPQGVVLLARRP
                     ASGPDTLDARPGVTPLVVVLHRVQNPSNAGAILRVAEAAGATAVVTTTRTADLFSPKA
                     LRGAMGSTFRLPVWAGAEFGEVLEWCARRGVRTVSTSLGAARTHTELDWRGPRAVVVG
                     SEGAGLGHEEALATDDSIRIPMRAPVESLNVAVAVAVVLYEAARQRSAL"
     gene            complement(1156..2118)
                     /locus_tag="VF240_13865"
     CDS             complement(1156..2118)
                     /locus_tag="VF240_13865"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7176345.1"
                     /translation="MRLPADIESPASPAGGDARGVEFGTFASETRAASTQRRVVVATF
                     NIRYAVGSFLITGGLLRRAGLSRPSRRSALVGGNILRAASLLADGERMPPADVVALQE
                     ADRRTTRAGARHVARELAASLRMNYAYAGMSVPRDVAPRTKQWYLNFEEPFGPGDAGD
                     TGVALLSRLPLEETTRIDLPWNDCPWRPRLALAASIPTEHGPVGVLNVHIDPHGPVEA
                     RLEQHRAVVATADRLFGSGPVVLLGDFNTLTREARDATRRLLESQGFSTPMPSGTATW
                     RAGLLRLHADWIFVRRARVHRWGVARVRGISDHWPVWAGIEAGD"
     gene            2193..2555
                     /locus_tag="VF240_13870"
     CDS             2193..2555
                     /locus_tag="VF240_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176346.1"
                     /translation="MKKQVIAVVDDLLFASKIRGAAEQAGAEVRFVKTADALLEAARS
                     GEPAVIILDLQTSLLDPFAATEMLKADEQTRGVSVVGFFPHVQADLQRRAQAAGIEHV
                     MPRSVFNQRLLEIVGGEL"
BASE COUNT          415 a          823 c          956 g          405 t
ORIGIN      
        1 cgcccgagct tgcggtcgag cacggagccg gcgctctcca gcaccctccg catcagccgc
       61 tcgacgagcg cctcgtcgcg cttcttttcc ttctctggac tcataatggc ctcggccgcg
      121 tcaccccgtt aaagaaaacg cccgccgaac tcggactaca gccgcgcctg cttgccgcgc
      181 cgcgcgccca ggggagaagt gtggcgaagg gttgaatagc ttggctgaca ttctagccga
      241 taaggggggt cgcggagaag caaaggttta gagtttgtct gtcaaaagtc ttccccgttg
      301 ctttcgcgga ggcggggatt cgtcgtgacg agacaccggg cggcgacggc cgtcagagcg
      361 cactcctctg ccgcgccgct tcgtacagga cgacggcgac ggcgacggcc acgttcagac
      421 tctccaccgg cgcgcgcatc ggaatccgta tcgagtcgtc ggtcgccagt gcctcctcgt
      481 gtcccagacc cgcgccctcg gagccgacga cgacggcgcg cggcccccgc cagtccaact
      541 cggtgtgggt gcgcgccgcc ccgagcgacg tgctcacggt gcggacgccg cgacgcgcgc
      601 accactcaag cacctcgccg aactcggcgc ccgcccagac ggggaggcgg aaggtcgaac
      661 ccatcgcgcc gcgcagggcc ttcggcgaga agaggtcggc cgtgcgtgtg gtggtgacga
      721 cggctgtggc cccggcggcc tcggccacgc gcaggatggc cccggcgttc gaggggttct
      781 gaacgcggtg caggacgacg acgagcggcg tcacaccggg ccgcgcgtcg agcgtgtcgg
      841 ggccggacgc ggggcggcgc gcgagcagga cgacgccctg cggcgacttc gtgtctgaga
      901 gttgccccat gacggcgtcg ctgacggcga cgactttcgc ggcggcggta ctgagcgagt
      961 cgagcaaacg ttgggcgcgt tcgtcgcgcg cgaaagtgtc ggtgtgcagg acggccgcga
     1021 cttcgagacc ggcgtgcgcg gcctcttcgc aaagacgaag gccctcgacg aagacgaggc
     1081 ccctgtcctt gccctcgcgc acctcgcggg cgcggcggac gagtgggttg tggcggctgg
     1141 tgatcttctc ttccatcaat ctcccgcttc aatccccgcc cacacgggcc agtgatccga
     1201 tatgccgcgg acgcgcgcga cgccccaacg gtggacacgt gcgcggcgga cgaaaatcca
     1261 gtcggcgtgc agacgcaaaa gaccggcgcg ccatgtcgcc gtgcccgagg gcatgggggt
     1321 tgagaagccc tgactttcaa ggagtcgccg cgtagcgtcg cgcgcctcgc gcgtcagcgt
     1381 gttgaagtcg ccgaggagga cgacggggcc gctgccgaaa agtcggtcgg cggtggcgac
     1441 gacggcgcgg tgttgttcga gacgggcctc gacggggccg tgggggtcga tgtggacgtt
     1501 gagaactcct acagggccgt gctccgttgg gatgctggcc gcaagcgcga gccgcgggcg
     1561 ccacggacaa tcattccacg gcaggtcgat gcgcgtagtt tcttcaagag ggaggcggct
     1621 gaggagcgcc acgcccgtgt ctcccgcgtc gccggggccg aacggctcct cgaagttcag
     1681 ataccactgc ttcgtgcgcg gcgccacgtc gcgcgggacg gacattccgg cataggcgta
     1741 gttcattcgg agcgacgcgg cgagttcgcg cgcaacgtgt cgggcgccgg cgcgggtcgt
     1801 gcggcggtcg gcctcctgaa gggcgaccac gtcggccggc ggcatccgct caccgtccgc
     1861 gagcagggag gcggcccgca ggatgttgcc gccgacgagc gcgcttcggc gcgacggccg
     1921 cgacaggccc gcgcggcgca ggaggccgcc ggtgatgaga aaggagccga cggcgtaacg
     1981 gatgttgaag gtggcgacga ctactctgcg ttgagtagag gcggctcgcg tctctgaagc
     2041 gaacgtgccg aactcgactc cgcgggcgtc gccgccggcg ggcgacgcgg gcgattcgat
     2101 gtcggcggga agtcgcatag aaggcttcag tctataatgt cgtcggacga aattttcagg
     2161 cgttgattga ttccgggcgg gggtgattat agatgaagaa gcaggtgatt gccgtggtgg
     2221 acgatctttt gttcgcgtcg aagattcgcg gggcggctga gcaggccggg gccgaggtgc
     2281 gattcgtgaa gacggccgac gcgctgttgg aggcggcccg gagcggggag ccggccgtca
     2341 tcatcctcga ccttcagacg agtttgctcg accccttcgc ggccacggag atgttgaagg
     2401 ccgacgagca aacgcgcggc gtgagcgtcg tcggcttctt tccgcacgtg caggccgact
     2461 tgcagcggcg cgcgcaggcc gccggaatcg aacacgtcat gccgcgctcg gttttcaatc
     2521 agcggctgct tgaaatcgtc ggcggagagc tataggccgc cggagtatgg caacagtacc
     2581 gcgagcggta gcgagcggg
//
LOCUS       DASVES010000340         9008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1563163, whole genome shotgun sequence.
ACCESSION   DASVES010000340 DASVES010000000
VERSION     DASVES010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9008
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563163"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1173
                     /locus_tag="VF240_13875"
     CDS             109..1173
                     /locus_tag="VF240_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7176347.1"
                     /translation="MATMRATVFHGKDDIRVEEVERPHAGVGEAVIRVTLTTICGTDL
                     HILRGEYPVRPGLVIGHEPVGVVEELGPGVVGYEVGDRVLVGAITPCGQCRGCLSGHL
                     SQCGHGDAFEALGGWRFGNTINGAQAEYLLVPHAQANLAKIPDGMTDEQVVLLADIAS
                     TGFSGAERGGVRIGDSVVVFAQGPIGLCSTVGARLMGAALVIGVDGDSNRLAMARRMG
                     ADVVLDYREVDVVAEVKKLTGGGADVTIEALGAQETFESALRSLRPGGTLSSLGVYSG
                     KLQVPYDAFAAGLGDHRIVTTLCPGGKERMRRLMEMVRSGRVDLTPLLTHTFSLENIV
                     EAYALFGERRDGVMKVAIKP"
     gene            1245..2240
                     /locus_tag="VF240_13880"
     CDS             1245..2240
                     /locus_tag="VF240_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7176348.1"
                     /translation="MRSLIRFILALCFVSAAATGAGQAQDDERLRIDTRLVSVHVSVT
                     DSRGRHVPGLTRDRFEIFDNKARREVAHFSAEEAPFSIGIVYDIHPSTPERVDAALRA
                     IKQFTATLRGEDDFFLMVFNERGSVVVEFVPTADQVTNHLTYAAPKGPASLYDAAFQA
                     AERLRSSRNVKKALLIISDGEDHDSGHSDKDVRDRARDFQIQVYGIGVADPADDPFAG
                     RGRWVLEDITGRTGRRAFPAGADASLGRAALDELSRTSGGTAYFPGGGVKGEQELAAV
                     CAQIALELRRQYALAFYPAEAPGAGWHKLSVRVTPPAGSGKLRLTYRKAYRPPER"
     gene            2247..3647
                     /locus_tag="VF240_13885"
     CDS             2247..3647
                     /locus_tag="VF240_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX7176349.1"
                     /translation="MSAHPCAAKHGAVSSDSPSSERRFAPPSRWLAAAAVIMSVFASS
                     LFWIENPLHARAVIVAGVCLTLWLSEAVPPFVPTLALWLLAPLLMQPLSEDFSLAKVL
                     GWSAEPVLALFLGGFALGVAARRHGIDALIAQVALRLSGGRRLALVALTAAVTACLSM
                     WMSNIAAAAMMISAVHPLVADVPADDRFRRALLLSIAVGANFGGIGTPVGTGPNAIAV
                     AAVSRHHTITFLDWMGFALPLAVGLIVAGVVILALRYRVRGAIRLPDLPRQPLSWSAR
                     CVVAVFLVTVAAWLTEPLHGASSALVALAASAVLFGTGLLDRQDLSRLDWSTLVLIAG
                     GIGLGNLLEKSGVVTAVAAALPWEQAPQLVRVLVLCSASAMLSALMSNTATVTMLIPL
                     AASLDPSPSTAILIAVAASLGMPFVISTPPNAMVYGEGGVKSSDLLLPGLVLMILGCL
                     VVGLTGPYVLKFIGIP"
     gene            3711..4124
                     /locus_tag="VF240_13890"
     CDS             3711..4124
                     /locus_tag="VF240_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176350.1"
                     /translation="MRTFKAGALYFLIAFAAGFALGPIRVLWAAPRFGERAAEMMEMP
                     LMLAVIIPAARWTVRRFAVRPSAGIGAGLIALGLLLAAEFTFVLWLRGLSISEYVAGR
                     DPVSGTAYALMLVVFALLPWLVARRRPNVTHHTRA"
     gene            4156..4893
                     /locus_tag="VF240_13895"
     CDS             4156..4893
                     /locus_tag="VF240_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176351.1"
                     /translation="MKENKTGAKVVGAGMAGAAALSAYLFVIRPWHLRWGATDEETRM
                     TLPGDEVTGDADYQVTHAVTVNAPADAIWPWLAQVGQGRGGFYSYSWLENLFGLRIRN
                     ADRVMPEFQHPEVGDFIRAAPPDWLGGRYAEYAGWHVVVLEPERAMVLRPHPDVEDNT
                     WAFVLKPVDGGKTRLIARARGKRKPSLSASLFNFFVGEPAHFIMERKMLLGIKRRVEG
                     ERAGSLGWGGNRLPLSLKDANPSRDKS"
     gene            complement(5049..5723)
                     /locus_tag="VF240_13900"
     CDS             complement(5049..5723)
                     /locus_tag="VF240_13900"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7176352.1"
                     /translation="MGFCNGMPSEEQIKADVLRELRQESRLKGTAIGVEVRDGTVTLT
                     GTVSSTSEILAALEAANRADTYFYLVNQIDLDAGVRPTDQEIAKSVMGALERDAVINE
                     HSLQVAISNGWVALSGKVERLREREEAERIARRTAGVRGVYNLIEVSPHTPSIENARD
                     AIVAALRRYAEQEAEGIDVDLRNGTADVSGKVHTWTEKQTVLGALTHAPGVERVKDHL
                     RVDPYF"
     gene            complement(5851..6006)
                     /locus_tag="VF240_13905"
     CDS             complement(5851..6006)
                     /locus_tag="VF240_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176353.1"
                     /translation="MRNNGSTNKKSVTIFGVMFGAVAGVAGLVAAAAGVIRHRKAIKR
                     LIEIERM"
     gene            6420..>9008
                     /gene="ppdK"
                     /locus_tag="VF240_13910"
     CDS             6420..>9008
                     /gene="ppdK"
                     /locus_tag="VF240_13910"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX7176354.1"
                     /translation="MSENQNGKWVYLFEEGDGRDKSLLGGKGAGLCEMTHAGLPVPPG
                     FVVTTAACNAFYRDGKQFPEGMWEQVVAGLRRVEEKTGKKFGDADNPLLVSVRSGAPF
                     SMPGMMDTVLNLGLNEQTVQGLAAQTGDLRFALDAYRRFASLFGEIVLGVAHDKFERV
                     MERYKAQTEGGRDTDLTPEQLREIIAAEKQIILAEQRGGAIPEDPYEQLKVAIAAVFN
                     SWMGRRAVDYRRVNRIREDLGTAVNVQAMVFGNMGASSATGVAFTRNPSTGERTLYGE
                     YLMNAQGEDVVAGTRTPQPITQLREELPEAYEQFDRITQLLERHYRDMQDVEFTIERG
                     KLWMLQTRTGKRTGAAAVRVAVDMVSEGLIDKATAVRRVTPEQLDQLLHPTVDPDAAA
                     SVVARGLPASPGAAQGKVVFDPDEAEEMARDGEKVVLVRQETSPDDFHGMVAAQAIVT
                     ARGGMTSHAAVVARGMGKSCVCGASELNIDYGQQQFNVNGTVVTKGEWVTVDGSTGRV
                     FLGRVPTVQPQLGEDFRELMCWADEFRRLRVRANADTPADARVARDFGAEGVGLCRTE
                     HMFFGEGRLAAMREMILADGRGAREKALEKLLPLQKNDFVGIFREMEGLPVTIRLLDP
                     PLHEFLPKMDELLGELSELKMSLQRAQTIREMDALLDRIDEKRKLLQQVERLHEANPM
                     LGHRGCRLGMMFPEVTRMQARAVFEAACQLTGEGRPVAPEIMVPLVSTAEELRRQREL
                     IDEVGRQVMGEHGMTIEYTVGTMIELPRAALMADSIAEHADFFSFGTNDLTQTTYGLS
                     RDDSGRFLSSYVETKVIPDDPFQVLDREGVGQLVRMGTERGRAAKPGLKVGICGEHGG
                     EPRSIAF"
BASE COUNT         1428 a         3005 c         3031 g         1544 t
ORIGIN      
        1 cactgctcgg tcgaagtcgt ccgcgagcaa aagcccgagt agagcgctcc ggccgacggc
       61 gaacactgag ttgaagccga cgggcgcagg ttacgacggg aggcacagat ggcaacgatg
      121 agggcgacgg tttttcacgg caaagacgac attcgcgtcg aggaggtgga gaggccgcac
      181 gcgggggtgg gcgaggccgt catccgtgtg acgctgacga ccatctgcgg caccgacctc
      241 cacatccttc gcggcgagta ccccgtccgc cccggcctcg tcatcggcca cgagccggtg
      301 ggagtcgtcg aggaactcgg gccgggcgtc gtcggttacg aggtcggaga tcgcgtgctc
      361 gtcggggcca tcaccccgtg cggccagtgc cgcggctgcc tctcagggca cctctcgcag
      421 tgcggccacg gcgatgcttt cgaggcgctg ggcggctggc gcttcggcaa cacgatcaac
      481 ggcgcgcagg ccgagtactt gctcgtgccg cacgcgcagg cgaacctcgc gaagattccc
      541 gacgggatga cggacgagca agtggtgctc cttgccgaca tcgcctcaac gggcttcagc
      601 ggcgcagaac ggggaggcgt ccgtatcggc gactcggtcg tggtcttcgc acaagggcct
      661 atcgggctgt gctcgaccgt cggcgcgagg ctcatgggcg cggccctcgt catcggcgtt
      721 gacggcgact cgaaccgcct ggcgatggcg cgccggatgg gcgccgacgt cgtgctcgac
      781 taccgcgagg tagatgttgt cgcagaagtg aaaaagctga cgggcggcgg ggcggacgtg
      841 acgattgaag cactcggcgc gcaggagacg ttcgagagcg ccctgcgatc tttaagaccc
      901 ggcggcacgc tctccagcct cggcgtctat tcggggaagc tgcaagtccc ctacgacgct
      961 ttcgccgcgg ggctcggcga ccaccgcatc gtgacgacgc tgtgccccgg cgggaaggag
     1021 cggatgcgcc gcctgatgga gatggtcagg agcggtcgcg tcgatctgac gccgctgctg
     1081 acgcacacct tctcgctgga gaacattgtg gaagcctacg cgctattcgg cgagcgacgc
     1141 gacggcgtga tgaaggtcgc gatcaagccc tgaagccgcg cccggcgcgc cttgctctgc
     1201 cgaagtggcc ctcgcgaatt agaagacaac agggagtgaa taccatgcgc agtctaatcc
     1261 gattcattct ggccttatgt ttcgtctcgg cggcggcgac cggggccggc caagctcagg
     1321 acgatgagcg cctccgcatt gatacccgcc tcgtctccgt gcacgtctcc gtgaccgact
     1381 ctcgcggccg ccacgtcccg ggtctcaccc gagaccggtt cgaaatcttc gacaacaagg
     1441 cgaggcggga ggtggcgcac ttctcggccg aagaggcccc gttctcaatc gggatcgttt
     1501 atgacatcca cccgtccacg cccgagcgcg tggacgccgc cctgcgcgcg atcaagcagt
     1561 tcacggcgac gctgcgcggg gaggatgact tcttcctgat ggtcttcaac gagcgcggga
     1621 gcgtcgtcgt agaattcgtc ccgaccgccg atcaggtcac gaatcacctg acgtacgccg
     1681 cgccgaaggg gccggcgtcg ctctacgacg cggcgtttca ggcggcggag aggctgcgct
     1741 cttcgcgcaa cgtgaagaag gcactcctga tcatttcgga cggggaggat cacgacagcg
     1801 gccacagcga caaggacgtg cgcgaccgcg cccgcgactt ccagattcag gtctacggta
     1861 tcggcgtcgc cgacccggcg gacgacccgt tcgccgggcg cggccggtgg gtgctcgaag
     1921 acatcacggg ccggacgggc aggcgcgcat tccccgcagg cgcggacgcg tcgctcgggc
     1981 gtgccgcgct cgacgagcta tctcgcacgt ccggcgggac ggcctacttc cccggcggcg
     2041 gggtcaaggg cgagcaggag ttggccgccg tgtgcgcgca gattgcgctg gagttgcgcc
     2101 ggcagtacgc gctcgccttc tacccggcgg aggcgcccgg cgccgggtgg cacaagctga
     2161 gcgtccgggt caccccgccc gccggctcgg ggaagctgcg cctcacctac agaaaagctt
     2221 accggccgcc cgagaggtga gagctgatga gcgcccaccc ttgcgccgcg aagcacgggg
     2281 ccgtgagttc agacagccca tcgagtgaga gacgcttcgc cccaccctcg cgttggctgg
     2341 cggcggccgc cgtcatcatg tcggtcttcg cgtcctcgct cttctggata gagaaccccc
     2401 ttcacgcccg cgccgtcatc gtcgccggcg tgtgtctgac gctctggctg agcgaagccg
     2461 tcccgccctt cgtgccgaca ctcgccctct ggctgctggc gccgctgctg atgcagcctc
     2521 tgagtgagga tttcagtctg gcgaaggtgc tcggctggtc ggcggagccg gtgctcgccc
     2581 tcttcctcgg cggcttcgcc ctcggcgtcg ccgcgcgccg gcacggcatc gacgcgctga
     2641 tcgcccaggt ggcgttaagg ctctcgggcg gtcgccgcct cgcgctcgtc gcgctcaccg
     2701 ccgccgtcac cgcctgcctt tcgatgtgga tgtcgaacat cgccgccgcc gcgatgatga
     2761 tctcggcggt gcatccgctg gtcgccgacg tgcctgcgga tgatcgtttt cgccgtgccc
     2821 tgcttttgag tatcgccgtg ggggcgaact tcggcggcat cggtacgccc gtcggcaccg
     2881 ggccgaacgc catcgccgtc gccgcagtct cgcgtcatca cacgataacc tttctcgact
     2941 ggatggggtt cgccctgccg ctggcggtcg gtttgatagt ggccggtgtg gtgatcttag
     3001 cgctgcggta tcgcgtgcgg ggggcaatca gacttccgga cttgccgcga cagcctctct
     3061 cctggagcgc gcggtgtgtc gtagcggttt ttctcgtcac cgtcgcggcg tggctgacgg
     3121 agcctctgca cggcgcttcg tccgcgctcg tcgcgctggc ggcgagcgcc gtgcttttcg
     3181 gcacggggct gctcgaccgc caagacctct cgcggctcga ctggtcaacc ctcgtgctca
     3241 tcgccggcgg gatcggcttg ggcaacctgc tcgaaaagtc gggggtggtg acggccgtcg
     3301 ccgccgcgtt gccgtgggag caggccccgc aactggtgcg cgtcctcgtg ctctgctcag
     3361 cgagcgcgat gctctcggcg ctgatgagca acaccgcgac cgtcacgatg ctcatcccgc
     3421 tcgccgcgag cctcgacccc tcgccttcga ccgccatcct gatcgccgtc gcggcgtctt
     3481 tgggaatgcc tttcgtcatc agcacgccgc cgaacgcgat ggtctacggc gagggcggcg
     3541 tcaagtcctc cgacctcctg ctgcccggcc tcgtgctgat gatcttgggc tgcctcgtcg
     3601 tcggcctgac cggcccatac gttttgaaat ttatcgggat cccctgagcc cgctcgggtc
     3661 accgacgctg agggccggcc gtgctgcgaa cggatgtgag gtgcaattcc atgcgaacat
     3721 ttaaggcggg cgcactttat ttcctcatcg ctttcgcggc cggctttgcg ctcgggccga
     3781 tccgcgtgct gtgggccgcc ccacgcttcg gcgagagggc cgcggagatg atggagatgc
     3841 ccctgatgct cgccgtaata atcccggcgg cgcgctggac ggttcggcgc ttcgcagtgc
     3901 ggccctctgc gggcatcggc gccggcctca tcgccctcgg cctcctgctc gcggcggaat
     3961 ttactttcgt cctctggctc cggggccttt cgatttccga atacgtcgcg ggccgtgacc
     4021 ccgtgtcggg caccgcctac gccctgatgc tcgtcgtgtt cgccctcctg ccgtggctcg
     4081 tcgcccggcg gcgcccgaac gtgacccatc acacacgcgc atgacgcgtg tgtcaaccac
     4141 gaaggagtga agaagatgaa agagaacaag acaggagcaa aagtcgtcgg ggcgggcatg
     4201 gccggtgcgg cggcgctctc cgcgtacctg ttcgtgatcc gcccctggca tctcaggtgg
     4261 ggcgcgaccg acgaggagac gcggatgacc ctgccgggtg acgaagtgac gggcgacgcg
     4321 gattatcagg tcacgcatgc cgtcaccgtc aacgcccccg ctgacgcaat ctggccctgg
     4381 ctggcgcagg tcgggcaggg gcgcggaggc ttctacagct actcttggct ggagaatctg
     4441 ttcggactgc gcatccgcaa cgccgaccgc gtgatgccgg aattccaaca cccggaggtc
     4501 ggggacttca tccgcgcggc tccccccgac tggctcggcg gcaggtatgc agagtacgcc
     4561 gggtggcacg tcgtcgtgct cgaacccgag cgcgcgatgg tgctgcgccc ccacccggat
     4621 gttgaggaca acacctgggc cttcgtcctg aaacccgttg acggcgggaa aacgcggctc
     4681 atcgcgcggg cgcgcggcaa acggaagccg tcgctttcgg cctcgctgtt taatttcttc
     4741 gtcggcgagc cggcccactt catcatggag cggaagatgc tgctggggat taagcgccgg
     4801 gttgaaggtg aacgggccgg ctcactggga tggggcggca atcgactgcc tctctcactg
     4861 aaagacgcga atccaagtcg cgataagtcg tgaatcgtga atgaagcggt caccattcag
     4921 ctttcggccg ggacgagcgg gccgcggtgc tgtgtgtcag cggcggcagc tctttcggcg
     4981 aatgccgttt gccgaaatgc tgggtgctga ccgcccgatg cacgattcac gacttacaat
     5041 tttcttctct aaaagtacgg gtcaactctc aaatgatctt tgacgcgctc gacgccgggc
     5101 gcgtgggtga gggcgccgag gacggtctgc ttctccgtcc aggtgtgcac cttgccggaa
     5161 acgtcggccg tcccgtttct caggtcaacg tcgatgcctt cggcctcctg ctcggcgtag
     5221 cgccggagcg ccgccacaat cgcgtcgcgg gcgttctcga tgctgggcgt gtgcgggctg
     5281 acctcgatga ggttgtagac gccgcgcacc ccggccgtcc ggcgcgcgat gcgctcggcc
     5341 tcctcgcgct cccgcaggcg ctcgaccttg ccgctgaggg cgacccaccc gttcgagatg
     5401 gcgacctgaa gcgagtgttc gttgatcacc gcgtcgcgtt cgagcgcgcc catcacggac
     5461 ttcgcgatct cctggtcggt gggccgcacg cccgcgtcga ggtcgatctg gttgacgagg
     5521 tagaagtagg tgtcggcgcg gttcgccgct tcgagcgcgg cgaggatttc ggaagtgctg
     5581 gagaccgtgc ccgtcagcgt cacggtgccg tccctgactt ccacgccgat ggccgtgccc
     5641 ttgaggcgcg attcctgccg aagctcccgc agcacgtccg ccttgatctg ctcttcgctg
     5701 ggcataccgt tgcagaatcc catcgctatc acctccgtca gaaaagcccg cgcatcacgt
     5761 tcgccactct cacgccggga agccaggtgg cgcgtgtgta tgggtgccga acttaaacgg
     5821 cactgctcca ctccgcgcgc gcccttccgg tcacatcctc tcgatctcga tgaggcgctt
     5881 gatggccttc ctgtggcgaa tcacgccggc cgcggccgcc accaaaccgg caacgccggc
     5941 caccgccccg aacatcaccc cgaaaattgt cacactcttt ttattcgtgc ttccgttgtt
     6001 tctcatggtg cttatctcct ttcgagaggg ccacgaggtc ggcccgttct tatcaggagc
     6061 aaggctcatg ccgtcgccgg gggaagcgtt gacgccgtgg cgcaaggtgt aaagaccagg
     6121 ctttgcgcgt gattaaaagc ctgagcctct cggcggcgga tcactccgcg ccggaatgtg
     6181 ggctttcacg cagcccgctg gtgaacttta cgcagcgcgg ccttgtggat gggcgccgct
     6241 gccgccccag tcgggccgaa cgcggtgggc acgtctcttg cactcttgga aaaggcgagt
     6301 ggtcggccga gcctttagtc gcgcgcccca ccgccgcaag tatcagggtc gcgcgagtct
     6361 ttggggccgg cgggcataaa attcggtttg aagtgaaatg tactggaaag agtgaacgca
     6421 tgtcggagaa tcagaatggc aagtgggtct acctgttcga ggagggcgac ggccgggaca
     6481 agtctctgct cggcggcaag ggcgcgggcc tgtgtgagat gacgcacgcg ggacttcccg
     6541 tcccgcccgg cttcgtcgtc acgaccgcgg cgtgcaacgc cttctaccgc gacggcaagc
     6601 agttccccga agggatgtgg gagcaggtgg tcgcagggct gcgccgcgtc gaagagaaga
     6661 ctggtaaaaa gttcggcgac gccgacaatc cgctcctcgt ctcggtacgc tccggcgcgc
     6721 ccttctcgat gccgggcatg atggacaccg tcctcaacct cggactcaac gagcagaccg
     6781 tgcagggcct cgccgcgcag accggagacc tccgcttcgc cctcgacgcc taccgccgct
     6841 tcgcctcgct cttcggagag atcgtcctgg gcgtggcgca cgacaagttc gagcgcgtga
     6901 tggagcgcta caaggcgcag accgaagggg gccgcgacac cgacctcacg ccggagcagc
     6961 tccgcgaaat catcgccgcc gagaaacaga tcatcctcgc cgagcagcgc ggaggggcca
     7021 tccccgaaga cccctacgag caactgaagg tggcgattgc cgccgtcttc aactcgtgga
     7081 tgggccgccg cgccgttgac taccggcgcg tcaaccgcat ccgcgaagac ctcggcacgg
     7141 ccgtcaacgt gcaggcgatg gtcttcggca acatgggcgc gtcttcggcg acgggcgtgg
     7201 cctttacgcg caacccctcg accggcgagc gcacactcta cggcgagtac ctgatgaacg
     7261 cgcagggcga ggacgtggtc gcgggcacgc gcacgccgca gcccatcaca cagcttcggg
     7321 aggaactgcc cgaggcttac gagcagttcg accgcatcac gcaactgctg gagcgccact
     7381 accgcgacat gcaagacgtg gagttcacca tcgagcgcgg gaagctgtgg atgctccaga
     7441 cgcgcaccgg caagcgcacg ggcgccgcgg ccgtgcgcgt cgcggtggac atggtgagcg
     7501 aaggcctgat tgacaaggcc acggccgtgc ggagagtcac ccccgagcag ttggatcaac
     7561 tcctccaccc gacggttgac ccggacgcgg ccgcgtcggt cgtcgcgcgg ggactgcccg
     7621 ccagccccgg cgcggcgcag ggtaaggtgg tcttcgaccc ggacgaggcc gaggaaatgg
     7681 cccgcgacgg ggagaaggtc gttctggtga ggcaggagac aagccccgac gacttccacg
     7741 gcatggtcgc cgcgcaggcc atcgtcaccg cccgcggcgg gatgacgagc cacgcggccg
     7801 tcgtcgcgcg cggcatgggc aagtcgtgcg tgtgcggcgc cagcgagttg aacatcgact
     7861 acggccagca gcagttcaac gtcaacggca ccgtcgtcac caagggcgag tgggtcacgg
     7921 ttgacggctc gacgggcagg gtttttctcg gccgcgttcc caccgtgcag ccccaactcg
     7981 gcgaggactt ccgagagctg atgtgctggg cagacgagtt ccgccgtctg cgcgttcgcg
     8041 ccaacgccga caccccggcg gacgcgcgcg tcgcgcgcga cttcggggcg gagggcgtcg
     8101 gcctgtgccg taccgaacac atgttctttg gcgagggccg cctggccgcg atgcgcgaga
     8161 tgattctcgc cgacggccgg ggcgcgcgcg agaaggcgct cgaaaaactc ctgccacttc
     8221 agaagaacga cttcgtcggc atcttccgcg agatggaggg actgcccgtc accatccgcc
     8281 tgctcgaccc gccgctccac gagttcctgc cgaagatgga cgagcttctg ggcgaactct
     8341 ccgagttgaa gatgtctttg cagcgcgccc agacgatcag ggagatggac gccctgctcg
     8401 accgcataga cgagaagcgc aagctcctcc agcaggtcga gcgtctgcac gaggccaacc
     8461 cgatgctcgg ccaccgcggc tgccgcctcg ggatgatgtt cccggaggtg acgcggatgc
     8521 aggcgcgcgc cgtcttcgag gccgcctgcc agctcacggg cgagggccga cccgtcgcgc
     8581 ccgagatcat ggtgccgctc gtctccaccg ccgaggagct gcgccgccag cgcgagctga
     8641 tcgacgaggt cggccgtcag gtgatgggcg agcacgggat gacgattgaa tacaccgtcg
     8701 ggacgatgat cgagctgccg cgcgcggccc tgatggccga ctcgatagcc gagcacgccg
     8761 acttcttctc cttcggcacg aacgacctca cccagacgac ctacgggctt tcgcgcgacg
     8821 actcgggacg cttcctctca tcctacgtcg agactaaggt tatccctgac gacccgttcc
     8881 aggtgctcga ccgcgaaggc gtcggccagc tcgtcaggat gggcacggag cgcgggcgcg
     8941 cggcgaagcc cggcctcaag gtcggcatct gcggcgagca cggcggcgag ccgcgctcca
     9001 tcgccttc
//
LOCUS       DASVES010000341         9458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1183577, whole genome shotgun sequence.
ACCESSION   DASVES010000341 DASVES010000000
VERSION     DASVES010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183577"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VF240_13915"
     CDS             <1..306
                     /locus_tag="VF240_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176355.1"
                     /translation="YFVLRGAAGEWVGSIAGSLLMGTVFAVGFGVARSALNLSAVLFV
                     ANSIGYFVGSALNDYVGGRAGMLLWGAAYGLCLGAGLGAVLHFAQAWRTRALTPERR"
     gene            319..867
                     /locus_tag="VF240_13920"
     CDS             319..867
                     /locus_tag="VF240_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176356.1"
                     /translation="MSGGEFLTRFTVWVAVAGYAAGTAAFALSRGRRRWDTAARLAWT
                     IGCASLLAHVACAFNFYHGWSHAAAYRDTARQTAGATGLDWGGGVYFNYALLIAWVAD
                     VAWWWRRGHDAYRRRPWLLTVAWHAFLIFMVFNATVVFASGVVRWAGLCVCLGLGVAW
                     WFAARDDSPRRPDARTLTVAKD"
     gene            872..2149
                     /locus_tag="VF240_13925"
     CDS             872..2149
                     /locus_tag="VF240_13925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7176357.1"
                     /translation="MNTLSAGMRRPVAGPFALPFYYGWVNLALAALAMVGTLPGRTQG
                     LGLITEQLLADLQIDRVVFARINLWATLAGALFCIGVGRLIDRFGSRVVLTVVAAALG
                     VVVLLMSGARGVASLALFITLTRGFGQSALSVVSITMVGQWFVRRLSLAMAVYTIALS
                     VGFMAAFPAVGAAVVGYGWRAAWMAVGLALLLGLAPLGWLLVRRSPEACGLQPDGERR
                     TVEQDGDAGALAGYTLWQALSTPAFWVFGLASAVYGLTASGIALFNESILAERGFDAS
                     VYHQSLVIVALTALVGNFTGGWLASRWTMNRLLALAMSLLAASLLALPHVRSRAHVAA
                     YAVVMGLAGGFVIVIFFTFWSRAYGRAHLGKIQGAAQALTVVASATGPLLLAECVTRT
                     GSYAAMFYALAAVVACLGLCAWLVPLPRESPSS"
     gene            complement(2146..2670)
                     /locus_tag="VF240_13930"
     CDS             complement(2146..2670)
                     /locus_tag="VF240_13930"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7176358.1"
                     /translation="MVPRTKWVERKFTFDLPAGCLANVLERVRGTSARLEDLTRGVSP
                     ELLTRRDGERWSMQEHAGHLLDLEELDTGRLDDFEAGLATLRAADMSNRKTREAAHNE
                     RRLTDILSELRHGRAEFVRRVEGYNEEFVSRPALHPRLQVPMRVIDLAYFISEHDDHH
                     LASISELLRQFGAR"
     gene            complement(2745..3812)
                     /locus_tag="VF240_13935"
     CDS             complement(2745..3812)
                     /locus_tag="VF240_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7176359.1"
                     /translation="MNITGVLTRAVLLLACVAGASGGARAQEGDRRGRAAAGTGADNA
                     ASRLTGVYRIDVAASDRLYSVVAGASSSLPFGEQQRFFLDLSTRLTPPDQLAIERRGR
                     HTIHIASSRAPRTTFEADARERREATADGYSLVRASLGNDQLVITSRSSSGEGYGVTF
                     TPLDGGRRLRVTRRISTRELREPVVIQSVYDRISEVARWEIYGEPPRGPGAAQRVVAA
                     VIPADPAAKFEAEWAEALRASLDDWVSATNARDIQRQMGYYMPTVQAFYLTRDVPARV
                     VRAEKARVFARARTIDVSAEEPEIIFRESGRVAVMRFRKRYRIEGGPGSRHGEVVQEL
                     RWRRTDEGWRIFSERDVRVIR"
     gene            complement(4037..4846)
                     /locus_tag="VF240_13940"
     CDS             complement(4037..4846)
                     /locus_tag="VF240_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX7176360.1"
                     /translation="MKKDHTQRRDGRPAHAWASPPTDVRASDDVVDLWRLPLGWPPRD
                     AEQFRQTLSADEAERAARFHFARDRDRFTAARALLRAVLALYLRTEPARLRFTYGPHG
                     KPALAGDAAGGDAAAALRFNLAHSHELALVAVARAREVGVDVEHVRPDFATAEVAERF
                     FSRREAEALRALPEGRERVEAFYRCWTRKEAYIKAKGEGLSFPLSSFTVSLAPGEPAA
                     LLGCDTDAGETERWTLVDLEPGADYFAAAAVEGRGWRARTFEAGMNLFERL"
     gene            5061..6617
                     /locus_tag="VF240_13945"
     CDS             5061..6617
                     /locus_tag="VF240_13945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7176361.1"
                     /translation="MKTDSGRSVSVWMATGDVPAREPLAEDTSADVCIVGAGIAGMTT
                     AYLLAREGKKVVVLDDGPIAGGETCRTTAHIVNALDDRYYDLEQWHGESGARLAAESH
                     TAAIDRVEEIVREEGIDCDFERVDGYLFVPPGESTEQLGEELRACHRAGLLEVEYVQR
                     VPYDGYDFGNALRFPRQAQFHILKYLDGVTRAFERLGGRVYTKTHAEKIEGDGGGEGE
                     GESRARVETSSRHAVTSDFVVVATNTPVNDLVSIHTKQAPYRTYVIGARVPSGSVPAL
                     LYWDTPDPYHYVRTQRGEGFDYLIVGGEDHKTGQAEDYDARFQKLEEWTRERFPMVES
                     VELRWSGQVMEPVDGMGYIGRNPGDRANVLIATGDSGNGMTHGTIAGVLLTDLVMGRD
                     NKWATLYDPSRVTVSADTGKEFAKENLNVAAQYADHLTGGEVGSVEEIEAGAGAVVRR
                     GLKKVAAYRDEEGKLYESSAVCTHLGCVVRWNDLEKSWDCPCHGSRFDCRDGHVLNGP
                     AISALSPAEE"
     gene            complement(6681..7457)
                     /locus_tag="VF240_13950"
     CDS             complement(6681..7457)
                     /locus_tag="VF240_13950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7176362.1"
                     /translation="MSKLQRMLGDGLPRPLQHPLEFLFDKRLSHAEQQAVWRVESIRQ
                     TVARRAGSFEAFNRDGKALRVSAPQIARTISVTPEGGTFLYLCSESFRARTILELGGC
                     AGISGCYLASSKYCERFITVEASPDLASLAGANLSLVSSRAEVVNAFFDDALDKILPT
                     FGDGLDLAYIDGHHKYETTLHYFRRLKPHLNIGGLIVFDDIHLSEGMRQAWQVLKGHE
                     GFAYTVGAGRFGIGLWDGSSTEPVHYNLCPYFGCLRKVSA"
     gene            7846..9141
                     /locus_tag="VF240_13955"
     CDS             7846..9141
                     /locus_tag="VF240_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176363.1"
                     /translation="MRLLTAAAIFHLALTMTIFGLGRHGVLPGTFNHDGIAVSFAPDG
                     VMFRDGAAGLSEILGRGEFINWATAHQPFHVKLYSICFALLGPLLGRNIISAEPLNLF
                     YYLTILTLVFILGREAFSRRVGLLAAIIVALWPSLLIHTTQMLRDQLFIIGMLTFILI
                     MLRWLTRIYSWPEALLRAAAGGATAILVWFARDNLAVIMVAAGLLGAGLLVARQFAQR
                     RAQTTNLVGMALMLGLTIGVTQAMPKYREPKDPQQRAEGFVNEASSGTPWGRIGSHVA
                     QARHQFILIYPDAGSNIDRDVRFNSLSDIIRYLPRAAAIGFFAPFPDMWFTSGKKVGS
                     AGRMLVGVECLMMYVVEVLAICGLWRGRRRASVWLLFLVSVVGMMALGLVVVNVGALY
                     RIRYLFLIMTIILAVEGAAAALGPMSRRLAGAEELSATP"
BASE COUNT         1520 a         3040 c         3174 g         1724 t
ORIGIN      
        1 tatttcgtcc tgcgcggcgc ggcgggcgag tgggtcggtt caatcgcggg gtctctgctg
       61 atgggcacgg tcttcgccgt ggggttcggc gtggcgcgct cggcgctgaa cttgtcggcc
      121 gtgctcttcg tagcaaactc catcggatac tttgtcggct cggcgcttaa cgattacgtc
      181 ggcgggcgcg cggggatgct tttgtggggg gccgcctatg ggctgtgtct cggggccgga
      241 ctcggggccg tcctgcactt cgcacaggct tggcgcacgc gagctttgac gccggagcga
      301 agatagtctg gggtctcgat gagcggcggc gaattcttaa cgaggttcac agtctgggtg
      361 gccgtcgcgg gctacgccgc cgggacggcc gccttcgcgc tctctcgcgg aaggcgcagg
      421 tgggacacgg cggcgcggct ggcgtggact attggctgcg cgagtctgct cgcacacgtc
      481 gcgtgcgcct tcaattttta tcacgggtgg agtcacgcgg cggcgtaccg cgacacggcc
      541 cggcagaccg ccggggcgac tggcctcgac tggggcggcg gagtctactt caactatgcg
      601 ctcctcatcg cgtgggtcgc cgatgtcgcg tggtggtggc gacgcgggca cgacgcctac
      661 cgccgacggc cgtggctgct cacggtcgcg tggcacgcgt tcctgatctt catggtcttc
      721 aacgcgacag ttgtttttgc gagcggcgtc gtgcgctggg cggggttgtg tgtgtgtctg
      781 ggcctcggcg tcgcgtggtg gttcgccgct cgtgacgatt caccgcgacg gccggacgct
      841 cggacgctca cggtcgcgaa agattgaacg catgaacacc ttaagcgccg ggatgcgccg
      901 ccctgtggcc ggccccttcg ccctcccgtt ctattacggg tgggtgaatc tcgctctggc
      961 ggcgctggcg atggtcggca cactgcccgg ccggacgcag gggcttggac tcatcaccga
     1021 acaattgctc gccgacctcc agatcgaccg cgtcgtcttc gctcgtatca acctctgggc
     1081 cacactcgcc ggcgcgctct tctgcatcgg cgtcggtcgt ttgatcgacc gcttcggcag
     1141 ccgcgtcgtc ctcaccgtcg tggccgcggc gctcggcgtg gtcgtccttc tgatgagcgg
     1201 cgcacggggg gtggcctcgc tcgcgctgtt catcacgctc acgcgcggct tcgggcagag
     1261 cgcgctctcg gtggtgagca tcacgatggt cggccagtgg ttcgtgcgcc gcttgagcct
     1321 cgcgatggcc gtctacacga ttgcgctgag cgtgggtttc atggcggcct tcccggcggt
     1381 gggcgcggcc gtcgtgggct acggctggcg cgcggcgtgg atggccgtcg gcctcgcgct
     1441 gctcctcggc ctcgcgccac tcgggtggct actcgtgcgg cgctccccgg aggcgtgcgg
     1501 tcttcagccg gatggcgagc ggcggacggt cgagcaagac ggcgacgcgg gcgcgctcgc
     1561 cgggtacacc ttgtggcagg cactctcaac gccggccttc tgggtgtttg gactagccag
     1621 cgccgtctac ggcctgacgg cctccggcat cgccctcttc aacgaatcga ttctggccga
     1681 gaggggcttc gacgcttccg tctatcacca gagcctcgtg atcgtcgcgc tgacggcgct
     1741 cgtcggaaac ttcacgggcg gctggctcgc gtcgcgttgg acgatgaatc ggctcctcgc
     1801 gctggccatg agcctgttgg ccgcgtcgct cctcgcgctg ccgcacgtta ggagtcgggc
     1861 gcacgtcgct gcttacgccg tcgtcatggg gctggccggc ggcttcgtca tcgtcatctt
     1921 cttcaccttc tggagtcgcg cctacgggcg cgcgcacctc ggcaaaattc agggcgcggc
     1981 gcaggcgctc acggtcgtcg cgtcggcgac ggggccgctg ctcctcgccg agtgcgtgac
     2041 gcgcaccggc tcctacgccg cgatgttcta cgcgctcgcg gccgtcgtgg cctgcctcgg
     2101 actgtgcgcg tggctcgttc cgcttccgcg tgaatccccg agttcttaac gcgcgccgaa
     2161 ctgccggagc agctcgctga tggaggcgag gtggtggtcg tcgtgctcgg agatgaagta
     2221 ggcgaggtcg atgacgcgca tgggaacctg caagcgcggg tggagggcgg ggcgggagac
     2281 aaactcctcg ttgtagcctt cgacgcggcg gacaaactcg gcgcgcccgt ggcgcagctc
     2341 cgagaggatg tctgtgaggc ggcgctcgtt gtgggcggcc tcgcgcgtct tgcggttcga
     2401 catgtcggcg gcgcgtaagg tcgcgagtcc ggcctcgaag tcgtcgagcc ggcccgtgtc
     2461 gagttcttcg aggtcgagga ggtggccggc gtgttcctgc atcgaccagc gttcgccgtc
     2521 gcgccgggtg aggagttccg gtgagacgcc gcgcgtcaaa tcttcgagcc gcgcggaggt
     2581 gccgcgcacg cgctctaaga cgttggcgag acagcccgcg gggaggtcga aggtgaactt
     2641 gcgctcgacc cacttcgtgc gtggaaccat tggggaacct ccgctcggaa ttatcgctcc
     2701 gagacggtgc ccgcgtccac gccgcgcagc acgagcggcg gcggtcagcg gatgacgcgc
     2761 acgtcgcgct cgctgaagat tctccagccc tcgtcggtgc ggcgccagcg cagctcctga
     2821 acgacttcgc cgtggcggct gccggggccc ccctcgatgc gataacgctt gcggaagcgc
     2881 atgacggcga cgcggcccga ctcgcggaag atgatctccg gctcctcagc gctcacgtca
     2941 atcgtgcgtg cgcgcgcaaa gacgcgggcc ttctcggcgc gcacgacgcg cgccggcacg
     3001 tcgcgcgtca gatagaaagc ctgcacggtc ggcatgtagt agcccatctg ccgctggatg
     3061 tcgcgcgcgt tcgtggccga cacccaatcg tcgagcgacg cgcgcagcgc ctcggcccac
     3121 tcggcctcga acttcgccgc cgggtcggcc gggatgaccg cggcgacgac cctttgcgcc
     3181 gcgcccggcc cccgcggcgg ctcgccgtaa atctcccacc gcgccacctc ggaaatcctg
     3241 tcgtagacgc tctggatgac gaccggctcg cgcagctcgc gcgtgctgat gcggcgcgta
     3301 acccgcaggc gacggccgcc gtcgaggggc gtgaaggtga cgccgtagcc ctcgccgctg
     3361 cttgagcgcg aagtgatgac gagctggtcg ttgccaagcg aggcgcgcac gagcgagtag
     3421 ccgtcggcgg tcgcctcgcg ccgctcgcga gcgtcggcct cgaaagtggt gcgcggcgcg
     3481 cgcgaggaag cgatgtggat ggtgtggcga ccgcgtcgct cgatggcgag ctggtcgggc
     3541 ggcgtcagac gcgtcgagag gtcgaggaag aatcgctgct gctcgccgaa gggcaggctg
     3601 ctcgaagccc cggcgacgac cgagtagaga cggtcgctcg cggccacgtc gatgcgatag
     3661 acgcccgtca ggcgcgaggc ggcgttgtcg gcgcccgtcc ccgccgccgc ccgtccccgc
     3721 cggtcgccct cctgcgcgcg cgcgccgccc gacgcgcccg cgacacacgc gagaagaagc
     3781 accgccctcg tcaacacccc cgttatgttc atcgccatac acctcacaag ttgttcgtcg
     3841 tgtccgcgcg cctcgcccgc accttgctgc tccgcatctg ctcaccggaa cacacaactg
     3901 actgccttta ttgccgctcg cccaatggtc gtgcggcggg cgagcggggg gttgaatttt
     3961 gataggggat tgacggatta agaatactaa cccgcccgtg gggtgtaaag gttgccggca
     4021 cgccgccgcg ttgtgctcac agtcgctcga atagattcat cccggcctcg aaggtgcgcg
     4081 cgcgccagcc acgcccctcg acggccgccg ccgcgaagta gtccgcgccc ggctccagat
     4141 cgacgagcgt ccaacgctcc gtctcgccgg cgtccgtgtc gcagccgagc agcgcggccg
     4201 gctcccccgg cgcgagtgag accgtgaatg agctgagcgg gaacgaaagc ccttcgccct
     4261 tcgccttgat gtacgcctcc ttgcgcgtcc aacacctgta gaacgcctcc acgcgctcgc
     4321 gtccttcggg cagtgcgcgc agcgcctcgg cctcgcggcg cgagaagaaa cgctcggcga
     4381 cctccgcggt cgcgaagtcc gggcggacgt gctccacatc gacgccgacc tcgcgagcgc
     4441 gcgccaccgc cacaagcgcg agttcgtgtg agtgggcgag gttgaagcgc agcgcggcgg
     4501 cggcgtcgcc gcccgccgcg tcgccggcca gcgccggctt gccgtggggg ccgtaagtga
     4561 agcgcagacg cgccggctcc gtcctcagat agagggcgag cacggcccgg agcagcgcgc
     4621 gcgcggcggt gaagcggtcg cggtctctgg cgaagtggaa gcgcgcggcc ctctccgctt
     4681 cgtccgcgga gagcgtctgc cgaaattgct cggcgtcacg gggcggccaa ccgagcggca
     4741 gacgccagag gtcaaccacg tcgtccgacg cgcgcacgtc cgtcggcggc gacgcccacg
     4801 cgtgcgccgg tcggccgtcg cggcgttgtg tgtgatcttt cttcacttga gtgttttaaa
     4861 ttcgtgtggg cggcgaccga gcgcgccgcg cgggcgaagg aaggattata cgcggcgccg
     4921 gaagattggt cagccctgct ctttcgcggg cgcgggccgc gaaaattttc tcgcgccggc
     4981 caaggcactt tgcctcctac gcgcgtcacg gtgtactttt aaggcaaatc aagacactgt
     5041 ctgtcgaggg ggaagacaac atgaagaccg attcggggcg tagtgtttct gtctggatgg
     5101 ccacgggcga cgtgcccgcg cgcgagccgc tggccgagga cacgtcggcc gacgtgtgca
     5161 tcgtcggcgc cggtatcgcg gggatgacga ccgcctacct gctcgcgcgc gagggcaaaa
     5221 aggtggtcgt cttagacgac ggccccatcg ccggcggcga gacttgtcgc acgactgctc
     5281 acatcgtcaa cgcgctcgac gaccgctact acgaccttga gcagtggcac ggcgagagcg
     5341 gcgcgaggct ggccgccgag agccacacgg ccgccatcga ccgcgtcgag gaaatcgtcc
     5401 gcgaggaggg catcgactgc gacttcgagc gcgtggacgg ctacctcttc gtcccgcccg
     5461 gcgaatcgac cgaacagttg ggcgaggagc tgcgcgcctg ccaccgcgcc gggctgctgg
     5521 aggtcgagta cgtccaacgc gtgccttacg acggatacga cttcggaaac gccctgcgct
     5581 ttccgcgaca ggcgcagttt cacatactta aatatctcga cggcgtgacg cgcgccttcg
     5641 agcgtctggg cgggcgcgtc tacacgaaga cgcacgccga gaagatcgaa ggcgacggcg
     5701 gaggcgaagg ggagggcgag tcgcgcgcgc gcgtcgagac ttcgagccgc cacgccgtca
     5761 cctccgactt cgtcgtcgtc gcgacgaaca cgccggtcaa cgacctcgtc tccatccaca
     5821 ccaagcaggc gccctatcgc acctacgtca tcggcgcgcg cgtgcccagc ggctccgtgc
     5881 cggcgctgct ctactgggac acgcccgacc cgtaccacta cgtccgcacc cagcggggcg
     5941 aaggcttcga ctatctcatc gtcggcggcg aggatcacaa gacggggcag gccgaggact
     6001 acgacgcgcg ttttcaaaaa ctcgaagagt ggacgcgcga gcgttttccg atggtcgagt
     6061 cggtcgagct gcgctggtcg ggtcaggtga tggagccggt ggacggcatg ggctacatcg
     6121 gccgcaaccc cggcgaccgc gccaacgtct tgattgccac gggcgactcc ggcaacggca
     6181 tgacgcacgg caccatcgcc ggcgtgctcc tcaccgacct cgtcatgggc cgcgacaaca
     6241 agtgggctac gctctacgac ccgtcgcgcg tcaccgtgag cgcggacacg ggcaaggagt
     6301 ttgcgaagga gaacttaaac gtcgccgcgc aatacgccga ccacctgacg ggcggcgagg
     6361 tcggctccgt cgaagagatc gaggcgggcg ccggcgccgt cgtccggcgc ggcttgaaga
     6421 aggtggccgc ctaccgcgac gaggagggga agctctacga gagttcggcc gtctgcacgc
     6481 acctcggctg cgtcgtgcgc tggaacgatc tggagaagtc gtgggactgc ccctgccacg
     6541 gctcgcgctt cgactgccgc gacggccacg tcttgaacgg cccggccatc agcgctttat
     6601 cgccggccga agagtgaact tccccgcccg tcgtggcggg gcttggggca tgatgcccga
     6661 tagatgagcc gcggacggtc ttaggcggac actttcctta aacagccgaa gtatgggcag
     6721 aggttatagt ggacaggctc cgtagaggag ccgtcccata agcctatacc gaacctgccc
     6781 gcgcccaccg tgtatgcgaa tccctcgtgc cctttgagga cttgccacgc ctgccgcatg
     6841 ccctccgaca aatgaatgtc atcaaaaaca atcaggcccc cgatgttcaa gtgcggcttg
     6901 aggcggcgaa aatagtgtaa cgtcgtctca tatttatgat gaccgtcgat ataagcgagg
     6961 tcaagaccat ctccgaacgt cggcagaatc ttgtccagag cgtcatcgaa aaatgcgttc
     7021 acaacctctg cgcggctcga aaccagggag aggttcgccc ccgccaatga agctaagtcg
     7081 ggcgatgctt ccacggtgat aaatctttcg caatacttcg acgatgccag gtagcaaccg
     7141 gaaattccgg cacaacctcc caactccaaa atcgtgcgcg ctctgaaaga ctccgagcag
     7201 aggtagagga atgtcccccc ttcgggggta acgctgatgg tgcgggcaat ctgtggggcg
     7261 ctaacccgga gagctttgcc gtcacggttg aaagcttcaa acgagcctgc ccgacgggcc
     7321 accgtctgcc gaatcgactc aaccctccac accgcctgct gttccgcgtg gcttaaccgc
     7381 ttgtcgaaca aaaactccaa cggatgttga agagggcggg gcaatccatc cccaagcatc
     7441 ctttgcagtt tagacatgcc gaaaggagcc acggcccgcc tgtaagccat tcgagcaaaa
     7501 gataaggact tgcgaaatgc tgtcattcag taacgagtga gacgaccata acgaccgtta
     7561 acccgcgcgg gttatctcaa gctagtttaa aaatagtaga agtctggcgc ccgccgcccg
     7621 ccggccgcgg cttttacgcc gcgggccaca acaatacccc cacgctgttg tccgccacca
     7681 ttttgatgta acttgtcggg ccatgatagc agccctcatc agatcggtgc gatacgggat
     7741 ttatattcta actttcgcgg gaccagtaag actgtgaagc aacggatgac ggggatcagg
     7801 gaagccttcc cggcgcgttg gagaaacagg gacgagcaca tccctgtgcg gctcctgaca
     7861 gccgcggcga tttttcatct ggcgctgacg atgaccatct tcgggctggg acggcacggg
     7921 gtcttgccgg gcacgttcaa tcatgacggg attgcggtgt cattcgcccc ggacggggta
     7981 atgtttcgcg acggggccgc cgggctgagt gaaattctcg ggcgcggcga gtttatcaat
     8041 tgggccacgg cgcatcagcc gtttcacgtc aagctctact cgatctgctt tgctctgctc
     8101 ggcccactgc tgggcaggaa tatcatcagc gccgagccgc tcaatctttt ctattacctc
     8161 acgattctga ctttggtctt cattctgggg cgggaggctt tcagccggcg cgtcggcctg
     8221 ctcgccgcca tcattgtcgc gctctggcct tcgctcttaa ttcacacgac gcaaatgctc
     8281 cgggatcagt tgttcatcat cgggatgctg acgttcattt taatcatgct gcgctggctg
     8341 acgagaatct attcgtggcc ggaagcgtta ttgagagcgg ccgccggggg ggcgacggcc
     8401 atcctggtct ggtttgccag agacaatctg gccgtcatca tggtcgcggc cggtctgctc
     8461 ggggcggggc tgctcgtggc ccggcagttc gcgcagcgcc gcgctcaaac gaccaatctc
     8521 gtcgggatgg ccttgatgct cgggctaacc atcggtgtta cgcaagccat gccaaaatac
     8581 cgcgagccga aggaccctca gcagcgcgcc gaaggattcg tcaacgaagc ctcgtcagga
     8641 accccttggg ggcgaatagg ctcacacgtt gctcaagcgc gccatcagtt tatcctgatc
     8701 tacccggacg ccggctcgaa cattgacaga gacgtgcggt tcaacagtct cagcgacatc
     8761 atccgttacc tgccgcgcgc cgccgccata ggtttctttg cgccgttccc cgacatgtgg
     8821 ttcacctcgg ggaagaaggt cgggtcggcc gggagaatgc ttgtcggcgt cgagtgtctg
     8881 atgatgtacg tggttgaagt tttagccatc tgcggcttat ggcgggggcg gcgtcgggct
     8941 tccgtctggt tgcttttctt agtgtcagtt gtcggcatga tggcgctcgg gctggtggta
     9001 gttaacgtcg gcgccctgta ccgcattcgc tatctgttcc tgatcatgac aatcatcctc
     9061 gccgtggagg gggccgcggc cgcgcttggc ccgatgtcga ggaggctcgc cggggctgaa
     9121 gagttgagcg ccacgccttg aagtctcagc gggcggcggc gtcttacagc ccgggtctta
     9181 aactcacgtt ttctgtctct ccagtgtgcc gggaaaaatt tcaccagacg gttgagaggg
     9241 agcaggtgac gacttcagtg aggaggtaca acattgcggc ggccgccatc aggcgggtct
     9301 tacgcggggt cggaaaagac gctgagaatc gctctatctg gcgccgcttc agtcgcaacc
     9361 tttcggtcgg gcttttgggg gcagtgctct ccctggccat taaatcagct caaaccctgc
     9421 tcctgacgag ggcgctccgg atggaggatt atgggcgc
//
LOCUS       DASVES010000342        12398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1210206, whole genome shotgun sequence.
ACCESSION   DASVES010000342 DASVES010000000
VERSION     DASVES010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210206"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..2488
                     /locus_tag="VF240_13960"
     CDS             401..2488
                     /locus_tag="VF240_13960"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7176364.1"
                     /translation="METFQRPELKLRDPADRETDKAALQAIAQAAVDVELFTLPLYMV
                     SMYSIQGTHQITSKGNDFYRGRLWPGFAPSRTPDASANAAAFNHFFSVFIDEMLHLQL
                     ASNIAKALGVMPSYNSPALQDDQYGWVCYGPDKTVLPHILDFKDAVEEYQDIRVKLDA
                     LTLEQNRLFLAIEQNEADAEKVIAKENQKKYFPTVPFENWKAGDALPLFGSIGAMYKC
                     LWQYIEIEYTDGVGLFEYVFNRGTLERDLFNTSTKYHKPEYPNMPTIASGEITVPDAM
                     ENILTMINAITDQGEGQGVAAEIRRARGRVRAGMLGYAPVEGQFRPDAEALDFDYPNY
                     TDQGQPDNPSGQKVARVENGGLDHHDRFLKIDELLKLKTDGVVTWDKWHAAGNEWTPE
                     MLITSEEDYKANKWNLPPADAVAAALNNLKANDPKGDNYKTFSQAAAGSIAGITRILS
                     GFWTNETTSFPMPSMYGSGDRVSICWAVFGKYPDLSGGIAPKKAGQLYHACQGLDLNG
                     KDDPAVCAQVAVYHSCRGSNTCKAEGGCGFVQVQGQSHQCGIKVILDHEQPTRPLPVQ
                     AQSQSSGPPQAGASTKPLFSAPSDNMCASFGGCAVPISASQLYPLPDGSDPEWTAAYM
                     ELFNFEGQTATPVLIKNKMGQKVLQKYDVGDRVYDIAWEAYIKVLKYRDPTQPVPDKP
                     EDGDLRLAFPPST"
     gene            2488..3435
                     /locus_tag="VF240_13965"
     CDS             2488..3435
                     /locus_tag="VF240_13965"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX7176365.1"
                     /translation="MQHTHPAAQQGRPPRRGLPNLGLGVGLRNQHFAYLMSHDPEVDW
                     FEIISENFIDNYGYARRVLERVASARPVVMHGVSLSVGSTDPLDWDYLKNLKALAEFL
                     RPAWISDHLCWTGVAGVNTHDLLPMPLNEESLKHVAARVRRVQDFLERPLVLENPSSY
                     LEFKDSTIAEWDFLSELARETDCGLLLDVNNVYVSSHNHGFDPEHYVRSLPHDAVVQI
                     HLAGPTDCGRFLVDTHDKPVPAPVWELYRLAQELTGGVSTLLEWDAEIPDFPALVAEL
                     RKAEAALKGENPGAPVQKSLAAAAAVSNPVGFQLAPSDE"
     gene            3428..4282
                     /locus_tag="VF240_13970"
     CDS             3428..4282
                     /locus_tag="VF240_13970"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX7176366.1"
                     /translation="MSEERLSLLQHWMKTVVTERGRLDEKLRAAALRHGLSAEDVVAE
                     RRGLSARERLAIYTGGYVLRLLECMRADFPVLRGFVGDPVFDAFARAYIITRPPSAPS
                     LYHLGADFPKFLQETRPSGERFDAGLSAMLDLPPEIARVERARAEVMRAHGTEDDPPP
                     AEPLSPFAVFSEGLRLEATGCLRLLELKFPLVDFLKSSDRGERPEAPAPRTTLVAVGR
                     SDYRVHVEEVTPWQFAFLKACERPAQLYEAVRDAALEAGKETPQVLAEVAVWLPVAFE
                     LGFLRRVA"
     gene            4478..4963
                     /locus_tag="VF240_13975"
     CDS             4478..4963
                     /locus_tag="VF240_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176367.1"
                     /translation="MNIGIVLALVLGGGGGALAASLCPHADCVPAADPVMTAAAEHHD
                     EASRGDHHGHTSEAAEGQHLDASFDTRQQNAPPATRQGVQSTHDRSCDHCVARRPAAP
                     ASELERRSDAPKRDNRVAPAPSARPVSAPAADFVTEVTPHQGAPPGRTSRHVLNNVFR
                     I"
     gene            5120..6859
                     /locus_tag="VF240_13980"
     CDS             5120..6859
                     /locus_tag="VF240_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7176368.1"
                     /translation="MDHSKRLRALTREVFCALVVVSLLVSAGPPAPAQHEGHTSPAPA
                     RPKAKKPARRQTQQRSAARRTTRRRAASRATRTSGAARRPAARPGSQAPAVDPHAGHA
                     QQATPTPTPAATPTPTPQTPTPAPAVDHSIHQTGQPQAPVRRRGSSGFIPEGPVVTLE
                     QLERMASERNPTLAQAAAFVRAAEGRRRQAGAFPNPVVGYFGEELAFRAAGETSEHGV
                     FVEQTISLGGKLGKSKRIFEREREQAEAIAESQRLRVTNSVRVLYYEALGAQQLVELR
                     ADLSALAREAVEITKELYNVGQADRPDQLEIEIEAERAEIESLRARTDWEQAWSALGA
                     LVNNPELQPARLAGSLEDGAAALDQEQLLIVLLRDSPEIRAARAGVERARAVLSRQRA
                     ERIPDLFVRGAVGYNNEILERDGRKTGAEGAIEVGVNVPIFNRNKGGVAAAEAELAIA
                     EREIDRLQLALRTRLASSFREYRNARTMVEKYRTQVVPRARQAYEMYLANFRQMAASY
                     PQVLIAQRTLFQVEVEYARALVEMRRSATALRGFLLGGGLDAVGRPGESGERTEGFRL
                     RSASEASGDADNR"
     gene            6912..8429
                     /locus_tag="VF240_13985"
     CDS             6912..8429
                     /locus_tag="VF240_13985"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEX7176369.1"
                     /translation="MDKSRRNFLRRGTLLGAAGVVAGSKVAHAQQHDAHAATPTPRAT
                     PKTGNRPAQTAGGGIVVPVHTPDVAKMPWTLENGVKVFHLTAEVVKTELLPGKQMYGW
                     GYNGSVPGPTIEVNEGDRVRFVFHNNLPEATTLHWHGLEVPLAMDGVPFISQPLVEPG
                     GMFTYEFTLHQNGTFFYHSHMPMQEMMGMIGFFIIHPKRPHAPRVDKDFGLILQEWAL
                     LPNNPIPNTLAMEFNWLTLNGKAGPATTPLIVRQGERVRIRMVNLGMDHHPMHMHGHQ
                     FYVTGTEGGRAPESTWFPGNTVLVGVAQARDIEFDAVYPGEWMLHCHLPHHMMNQMVS
                     MVGPMAHAGRGSQTGLGMEEGMGVVRQGHALSEDIGPGFGRGTGDTTSQERNTSNLVG
                     PSQQQQGVQTPPSQEQGKGTQVQPEQQSTLGDDASKGRVPGYPQDMIMFMDKEVAKPE
                     TYGLPPGWTASMMGMMTLVRVLPPDVYDEVQRRIREGITEPPQGQPAGVQPHKHE"
     gene            complement(8450..10306)
                     /locus_tag="VF240_13990"
     CDS             complement(8450..10306)
                     /locus_tag="VF240_13990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7176370.1"
                     /translation="MTTDEAHAQTRHETSAQRRPVRRARLFVLCAVVFLVALGVRALH
                     WQDSRHAFIYEGMAVEYKEHALLLYEGDVTRFVRGDEWPLQASIIKHPPGYPLLLAAV
                     YSVFGVSDTPVRALHIVLDALAAVLVLLLALELFGSAAVATIAGLLVALSPQFAYHSI
                     ALVPDPLAAPPILLAFYLLARTGKRPRLMSVAAAGAMIGVSCWLRSNALLLAPFVALL
                     APLLFERGRRLRPALVLAAAGLLVIVPVTIRNAVVYKAFIPLSLSAGITLVEGIGIYD
                     REGRFGLPSSDYAVTKWEAEMYGRPDYLGSRFDPDGVERERSRISHGLAVVRAHPFWF
                     LRVMAHRAASMWRLARVEIVAAEPAVANAPNPGEGERPAWTIEPSGLTKDGDTAAQSE
                     LRTTGDSSALRFAGEQMGTLVVSPPVAVQKNTDYLLRLPLKIEQGSIVVEVRDARRDA
                     VLASTPILHPIDYLDFTRDTQPAVNIMRPFVSGDAEEVRVVVRNASQRPVAAEAGRVE
                     GFALGPSAHAWTRPVRLLVRGAQKLFLTAFVLPFIILGLFLLVRARRWGAIALLLSVP
                     VYYMCTQSALWTEFRYILPMHYFLFILAALGLQQTFMFLRRAVLKLRGGRVA"
     gene            10462..11988
                     /locus_tag="VF240_13995"
     CDS             10462..11988
                     /locus_tag="VF240_13995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7176371.1"
                     /translation="MPWFMNVEELNLVNTASASPADETEVRGADAARLRYRRERAALW
                     DAQARRMDTWRGWGGAYHRRLEELYRFFVPPGSRVLDIGCARGDLLAALEPSVGVGVD
                     FSTEMVERARKRHPHLRFLQADAHELEIEGEFDFIILSDVVNDLWDVQEVFRRVGQLS
                     GPRTRVVLNFYSRLWELPLALAQRAGAATPQLPQNWLTVEDVSGLLSLADFEVIRRRD
                     EILLPLPVPPLSTLCNRYLVKLWPFNVFALTHVLVARPAGRVEQQPAAAAAVPRVSVV
                     VPARNEAGNIADIFRRTPEMGSETELIFVEGGSTDDTYETIEREMAAHPTRSARLLRQ
                     TGRGKGDAVRTGFAHATGDVLMILDSDLTVPPEDLPRFLEVLTSGKADFANGSRLVYP
                     MEREAMRFLNLVGNKFFSLAFSWLLGQPVKDTLCGTKVLRRDDYERIAAGRAYFGDFD
                     PFGDFDLLFGASKQNLKLLDVPVRYRDRTYGATNISRWSHGWLLLRMVAFAARRIKFI
                     "
BASE COUNT         2255 a         4169 c         4094 g         1880 t
ORIGIN      
        1 gtggcgaagc ccgtcgagcc gatggagttg gcggccgtcg tggcgagcct cgccgggcgc
       61 accggaacga agaagtcaga tcatcaccgt ttgatattta ctgccgatac tcggatatgg
      121 cttatgggac gccgtcaggg aactctcact caggcgtcgc cgccgaagtg aaaagacccg
      181 agagatcctt ctcacctcag agtaatttct gccacgctac cgtttgttcc gtgtcccatg
      241 tagccgagac aaagttcttg ctttttcaag agttgcggaa gtaacatccg cccggcatat
      301 ctcaccagtg gccctgaacc gggtcggaat tcgcgagaga catacacaag cagtctgaca
      361 caccttgttc tcactccagc cggaacccac aaggagaaac atggaaactt ttcaacgccc
      421 ggaattgaaa ctgcgcgatc ccgccgacag ggagaccgac aaggccgccc tccaggccat
      481 cgcccaggcc gccgtcgatg tggagctgtt caccctcccg ctctacatgg tctcgatgta
      541 ctccatccag gggacgcacc agatcaccag caagggcaac gacttctacc gcggccggct
      601 ctggcccggc ttcgccccct cgcgcacgcc cgacgccagc gccaacgccg ccgccttcaa
      661 tcacttcttc agcgtcttca tcgacgagat gctgcacctc cagctcgcct cgaacatcgc
      721 caaggcgctc ggcgtcatgc cgagctacaa cagccccgcc ctccaggacg atcaatacgg
      781 ctgggtctgc tacgggccgg acaagacggt gctgccgcac atcctcgatt tcaaggacgc
      841 ggtcgaagaa taccaggaca tccgcgtcaa gctcgacgcg ctcacgcttg agcagaaccg
      901 cctcttcctc gccatcgagc agaacgaggc tgacgccgag aaggtcatcg ccaaggagaa
      961 tcagaaaaaa tacttcccca cggttccctt cgagaactgg aaggcgggcg acgccctccc
     1021 gctcttcggc tccatcggcg cgatgtacaa gtgcctctgg cagtacatcg agatcgagta
     1081 cacggacggc gtcggcctct tcgagtacgt cttcaaccgc ggcacgctcg agcgcgacct
     1141 cttcaacacc tccaccaagt accacaagcc cgagtatccc aacatgccca ccatcgcgtc
     1201 gggcgagatc accgtccccg acgcgatgga gaacatcctg acgatgatta acgccatcac
     1261 cgatcagggc gaggggcagg gcgtggccgc cgagatcagg cgcgcgaggg gacgtgtgcg
     1321 cgccgggatg ctcgggtacg cgcccgtcga ggggcagttc cgccccgacg cggaggcgct
     1381 cgacttcgac tatccgaact acaccgacca gggccagccg gacaacccct cgggccagaa
     1441 ggtcgcgcgc gtcgagaacg gcgggctcga ccaccacgac cgcttcctga agattgacga
     1501 gctgctgaag ctgaagacgg acggcgtcgt cacctgggac aagtggcacg cggcggggaa
     1561 cgagtggacg cccgagatgc tcatcacgag cgaggaggac tacaaggcga acaagtggaa
     1621 cctgccgccg gccgacgccg tggccgccgc gctcaacaac ctgaaggcga acgatcccaa
     1681 gggggacaac tacaaaacct tcagccaggc ggccgccggc tccatcgccg gcatcacgcg
     1741 catcctctcc ggcttctgga cgaacgagac gacgagcttc cccatgccct cgatgtacgg
     1801 ctcgggcgac cgcgtctcga tctgctgggc cgtcttcggc aaataccctg acctctccgg
     1861 cggcatcgcc ccgaagaagg cggggcagct ctaccacgcc tgccaggggc tcgacctgaa
     1921 cggcaaggac gacccggccg tgtgtgcgca ggtcgcggtc taccactcgt gccgcggctc
     1981 gaacacttgc aaggccgagg gcggctgcgg cttcgtgcag gtgcaggggc agagccacca
     2041 gtgcggcatc aaggtcatcc tcgaccacga gcagccgacg cggccgctgc ccgtgcaggc
     2101 gcagtcgcag tcgtcggggc cgccgcaggc gggcgccagc accaagccgc tcttcagcgc
     2161 gccctcggac aacatgtgcg cgagcttcgg cggctgcgcc gtccccatct cggcctcgca
     2221 gctctacccc ctgcccgacg gttcagaccc ggaatggacg gccgcctaca tggaactctt
     2281 caacttcgaa ggccagacgg ccacgccggt tctgatcaag aacaagatgg gtcagaaagt
     2341 gctacagaag tacgatgtgg gcgaccgcgt ctacgacatc gcgtgggagg cgtacatcaa
     2401 ggtgctcaag taccgcgacc ccacacagcc cgtgcccgac aagcccgagg acggcgacct
     2461 gcggctggcc ttcccgccct ccacgtgatg cagcacacgc accccgcagc acagcagggt
     2521 cggccccctc gccgaggtct cccgaacctc ggcctggggg tcggcctgcg caaccagcac
     2581 ttcgcctacc tgatgagcca cgaccccgaa gtggactggt tcgagatcat cagcgagaac
     2641 ttcatcgaca actacggcta cgcgcgccgc gtgctggagc gcgtagcgtc ggctcgcccc
     2701 gtcgtcatgc acggcgtctc gctctccgtc ggcagcaccg acccgctcga ttgggactac
     2761 ctgaaaaact tgaaggcgct cgcggagttc ctccgccccg cgtggatctc cgaccacctg
     2821 tgctggacgg gcgtcgcggg cgtgaacacg cacgacctcc tgcccatgcc gctcaacgag
     2881 gagagcttga agcacgtcgc ggcgcgcgtc cggcgggttc aggacttttt agagcgaccg
     2941 ctggtgctcg aaaacccttc gagttatctg gagttcaagg actcgaccat cgccgaatgg
     3001 gacttcctct cggagttggc gcgcgagacg gactgcgggc tgcttttaga cgtgaacaac
     3061 gtctacgtgt cgagccacaa ccacggcttc gaccccgagc actacgtccg cagcctgccg
     3121 cacgacgcag tcgtccaaat ccacctcgcg ggcccgacgg actgcggccg cttcctcgtg
     3181 gacacgcacg acaagcccgt gcccgcgccc gtgtgggagc tgtacaggct ggcgcaggaa
     3241 ttgacgggcg gcgtctcgac gctccttgag tgggacgctg aaatcccgga ctttcccgcg
     3301 ctggtcgcgg agctgcgcaa ggccgaggcg gctctcaagg gagagaaccc gggcgcgccg
     3361 gtgcagaagt cgctcgcggc ggcggcggcc gtctccaacc ccgtcggctt tcagttggcg
     3421 ccgagcgatg agtgaagagc gcctgagcct cttgcagcac tggatgaaga cggtcgtcac
     3481 ggagcgcggc cgactggacg agaagctgcg cgcggccgcg ctgcggcacg ggctgagcgc
     3541 cgaggacgtg gtggccgagc ggcgcggcct ctccgcgcgg gagcggctcg ccatctacac
     3601 gggcggctac gtgctccgtc tgctcgaatg catgcgcgcg gacttccccg tcttgcgcgg
     3661 cttcgtcggc gacccggtct tcgacgcctt cgccagggcg tacatcatca cgcgcccgcc
     3721 gagcgcgccc agcctttacc acctcggcgc ggacttccca aaatttttgc aggagaccag
     3781 accgagcggc gaacgcttcg acgccgggct gagcgccatg ctcgaccttc cgccggagat
     3841 tgcgcgcgtc gagcgagcgc gggccgaggt catgcgcgcc cacggcacgg aggacgatcc
     3901 accgccggcc gagccgctct cgcccttcgc ggtcttcagc gaggggctac ggcttgaggc
     3961 gaccggctgc ctgcgtctgc ttgagttgaa gttcccgctg gtcgattttc tgaagagttc
     4021 ggacaggggc gagcgccccg aagcgcccgc gccgcgcacc acgctcgtcg ccgtgggacg
     4081 ctccgactac cgcgtccacg tcgaagaggt cacgccctgg cagttcgcct tcctcaaggc
     4141 ttgcgagcgc cccgcgcagc tctacgaagc cgtgcgcgac gcggcgctcg aagccggcaa
     4201 ggagacgccc caggtgctcg ccgaggtcgc tgtctggctc cccgtcgcct tcgagctggg
     4261 cttcctgcgc cgcgtcgcct gaaaacctta tcggttttat tgcagcaacg gcccctacct
     4321 gaatagtgat gtaatgaagc aggcgcacgc aaaatcgcta taagagggtt ctttcgcgct
     4381 gtttcaagtt gtgttagtga aaacttcccg gcgtggacat gcatcaaatt gtgtggtaat
     4441 taaacccata aggcgaaacc ctgtgacaag tcggctggtg aacattggca tcgtgttggc
     4501 actggtgctg ggcggaggtg gaggcgcgct cgccgcatct ctgtgcccgc acgccgactg
     4561 tgtccccgcc gccgacccgg tcatgacggc cgccgcggag catcacgacg aggcttcgcg
     4621 tggcgatcat cacggacaca catcggaggc cgcggagggc caacacctcg acgcgtcctt
     4681 cgacacgcga cagcagaacg ccccgcccgc gacccgacag ggcgttcaaa gcacgcacga
     4741 tagatcgtgc gaccactgcg tcgcccgacg cccggccgcg ccggcgtccg aacttgagcg
     4801 gcgctcagac gcgccgaagc gtgacaaccg cgtcgcaccc gcgccctccg ccagacctgt
     4861 gtcggcgccg gccgccgact tcgtcactga agtcaccccg catcagggcg ccccgcccgg
     4921 tcgcaccagc agacacgtcc tcaacaacgt cttccgcatt tagacccgcc cgcgcgggct
     4981 tcggccgagg tgcgccgccc gccaacccct gcgcttcagt ctctctttaa aacttctgcc
     5041 cggtagacac gacgcggcgc gtccgatgtt cggcgcacgc tcgggtctgc ttttgttacc
     5101 cacaggggag gaattcgtca tggaccactc taagcgcctc agggcgctga cgcgcgaggt
     5161 cttctgcgcg ctcgtcgtcg tctctctgct cgtctcggcg gggccgcccg cgccggcgca
     5221 gcacgagggg cacactagcc ccgcgcccgc gaggccgaaa gctaaaaagc cggcccggag
     5281 gcaaacacaa caaaggtcgg ccgcgcgcag aacaacgcgg cgtcgcgcgg cgagcagggc
     5341 tacgcggact tcgggcgcgg cgcggcgacc ggctgcgcgt cccggcagtc aggcaccagc
     5401 cgtcgatccg cacgccgggc acgcgcagca agccacgccc actccgacgc cggccgcgac
     5461 gccgacaccg acgccgcaga cgccgacgcc cgcgccggcg gtggatcaca gcattcatca
     5521 aaccggacaa ccgcaggcgc cggtgaggag gcgtgggagc agcggattca tccccgaagg
     5581 ccccgtcgtg acgctcgaac agttggagag gatggcgtcc gagaggaacc cgacgctcgc
     5641 gcaggccgct gccttcgtcc gcgccgccga aggccgacgc cgacaggcgg gcgctttccc
     5701 taaccccgtc gtcggttact tcggcgagga actggccttc cgcgccgccg gcgagacgag
     5761 cgagcacggc gtcttcgtcg agcagacgat ttcattgggc gggaagctgg gcaagtcaaa
     5821 gcgcatcttc gagcgcgagc gcgagcaggc cgaggccatc gcggagtcgc agcgcctgcg
     5881 cgtcacgaac tccgtccgcg tgctctatta cgaggccctc ggcgcacagc agttagtcga
     5941 actccgcgcc gacctgtcgg ccctcgcgcg cgaggccgtc gagatcacca aggagcttta
     6001 caacgtcggg caggccgacc gccccgacca actggagatc gagatcgagg ctgagcgcgc
     6061 cgagatcgaa agcttgcgcg cgcgcaccga ttgggagcag gcgtggagcg cgctgggggc
     6121 gttggtgaac aacccggagc tgcagcccgc gcgcctcgcc ggcagcctcg aagacggggc
     6181 cgccgcgctc gatcaggagc agttgctcat cgtcctgctg cgcgacagcc cggagatcag
     6241 ggcggcccgc gccggtgtcg aacgcgcgcg cgccgtgctc tcgcgccaga gggccgagcg
     6301 catccccgat ctcttcgtcc gtggcgccgt cggttacaac aacgagattc tggaacgcga
     6361 cggcaggaag acgggggcgg aaggtgccat cgaagtcgga gtcaacgtcc ccatctttaa
     6421 tcgcaacaag ggcggcgtcg cggcggccga ggccgagctg gccatcgccg agcgggaaat
     6481 cgaccgcctc caactcgcct tacgcacgcg cctcgcttcc tccttccgcg aataccgcaa
     6541 cgcccggacg atggtcgaga agtaccgcac gcaggtcgtc ccgcgcgcgc gccaagctta
     6601 cgagatgtac ctcgcgaact tccgccagat ggccgcctcc tacccgcagg tcttgatcgc
     6661 ccagcgcacg ctctttcagg tcgaggtcga atacgctcgc gcgctcgtgg agatgaggcg
     6721 gagcgccacg gccctgcgtg ggtttctgct tggaggcgga ctcgacgccg tgggccgccc
     6781 cggcgagtca ggcgagcgca ccgaaggttt caggctgaga tcggcgagtg aagcgagcgg
     6841 cgatgcggac aaccgctaaa gttttcgccg cagttatttg tcaaagggtt caaacataca
     6901 ggagtaagaa gatggacaag agtagacgta attttctaag acgcggaaca ctcctcggcg
     6961 ccgcgggagt tgtggctggt tctaaagtcg cccacgcgca acagcacgac gctcacgccg
     7021 cgacgccgac gccgcgggcc acgccgaaga ccggcaatcg tcccgcgcaa acggcgggcg
     7081 gcggcatcgt cgtccccgtc cacacgcccg acgtggcgaa gatgccttgg acgttggaga
     7141 atggagtgaa ggtcttccac ctcacggcgg aagtcgtcaa gaccgagctt ttgccgggca
     7201 agcagatgta cgggtggggc tacaacggca gcgtccccgg cccgacgatt gaagtcaacg
     7261 agggcgaccg cgtccgcttc gtcttccata acaacctgcc cgaagcgacc accctccact
     7321 ggcacggcct cgaagtgccg ctcgcgatgg acggcgtgcc cttcatcagc cagccgctcg
     7381 tcgagccggg cgggatgttc acctacgagt tcacgctcca ccagaacggc accttcttct
     7441 atcactcgca catgcccatg caggagatga tgggcatgat cggcttcttc atcatccacc
     7501 cgaagcgccc ccacgcgccg cgcgtggata aagacttcgg gctgattctg caagagtggg
     7561 cgctgctgcc gaacaacccc atcccgaaca ctctggcgat ggaattcaac tggctcacac
     7621 tcaacggcaa ggccggcccg gcgacgacgc cgttgatcgt ccgtcagggc gagcgcgtgc
     7681 gtatccgcat ggtcaacctc ggcatggatc accacccgat gcacatgcac ggccaccagt
     7741 tctatgtgac ggggacggag ggcgggcgcg cgcctgaatc gacgtggttc cccggcaaca
     7801 ccgttctggt cggagtggct caggcgcgcg acatcgagtt cgacgccgtc tatcccggcg
     7861 agtggatgct ccactgccac ctgccgcacc acatgatgaa tcagatggtc tcgatggtcg
     7921 ggccgatggc gcacgccggg cgcggctcgc agacggggct tgggatggaa gagggcatgg
     7981 gcgtcgtccg gcagggacac gcgctctccg aggacatcgg gccgggcttc gggcgcggga
     8041 cgggcgacac cacctcgcag gaaagaaaca cctcgaacct cgtggggccg agccagcagc
     8101 aacaaggcgt tcagacgccg ccgagtcagg agcaggggaa gggcacgcag gtacagcccg
     8161 aacaacagag cacgcttggc gacgacgcca gcaaagggcg ggtgcccggc tacccgcagg
     8221 acatgatcat gttcatggac aaggaagtgg ccaagccgga gacctacggc ctgccgcccg
     8281 gttggacggc ctcgatgatg ggcatgatga cgctcgtgcg cgtgctcccg cccgacgtgt
     8341 acgacgaagt ccagcgtcga atcagggagg gcatcacgga gccgccgcag ggtcaaccgg
     8401 caggtgtgca gccgcacaag cacgagtaat caagggcgtg cgcggaatgt caggcgacgc
     8461 gtccgcctcg cagcttcagg acggcccggc gcaaaaacat gaacgtctgt tggaggccga
     8521 gcgcggcgag gatgaagagg aagtagtgca tggggaggat gtagcggaac tccgtccaca
     8581 gggctgactg tgtacacatg tagtagacgg ggacggagag gaggagggcg atagcacccc
     8641 agcggcgtgc gcggacgagc aggaagaggc cgaggatgat gaaggggagc acgaaggcgg
     8701 tgaggaatag tttctgggcg ccacggacga ggagacgcac cggacgcgtc cacgcatgag
     8761 cggaggggcc gagggcgaag ccttcgacgc ggcccgcctc ggcggcgaca ggtctctggc
     8821 tggcattgcg tacaacgaca cgcacctctt cagcgtcgcc gctcacgaag ggtcgcatga
     8881 tattgacggc gggctgcgtg tcgcgcgtga agtcgaggta gtcaatcgga tgcaggatgg
     8941 gtgtggaggc gagcacggcg tcgcggcgcg cgtcccggac ttcgacgacg atgctgccct
     9001 gttcgatctt gagcgggagg cgaagcaggt agtcggtgtt cttctggacg gcgacgggcg
     9061 gggagacgac aagggttccc atctgttcac ccgcgaagcg gagcgcggaa gagtcgccgg
     9121 tcgtgcggag ttcggactgg gcggcggtgt cgccgtcttt cgtcaaaccg gaaggttcaa
     9181 tcgtccacgc cggccgctcg ccttcgccgg ggttgggcgc gttggcgact gccggctcgg
     9241 ccgcgacgat ctcgacgcgc gcgagcctcc acatcgacgc ggcgcggtgg gccatcacgc
     9301 ggaggaacca gaaggggtga gcgcgcacga cggcgagacc gtgactgatg cggctgcgct
     9361 cgcgctcaac gccgtccggg tcgaagcgcg agccgagata gtcggggcgg ccgtacatct
     9421 ccgcctccca cttcgtcacg gcgtagtctg aagagggcag accgaagcgc ccttcccggt
     9481 cgtaaatccc gatgccctcg acgagagtta tccccgcgct caaagagagc gggatgaacg
     9541 ctttgtagac gacggcgttg cgaatcgtca cgggcacgat gacgagcagc ccagcggcgg
     9601 cgagcacgag cgccgggcgc agacgccggc cgcgctcgaa cagcagcggc gcgagcagtg
     9661 cgacgaaggg cgcgagcagc agcgcgttcg acctgagcca gcaggagacg ccgatcatgg
     9721 cgccggccgc cgccacgctc atcagacgcg gtcgcttccc cgtgcgcgcg agcagataga
     9781 aagcgagcag gatgggcggg gcggcgagcg ggtcggggac gagcgcgatt gagtggtagg
     9841 cgaactgcgg cgagagcgcg acgagcaggc cggcgatggt ggcgacggcg gcgcttccga
     9901 acagttcgag cgcgagcagg aggacgagca cggccgccag cgcgtcgagc acgatgtgga
     9961 gagcgcgcac cggcgtgtca gagacgccga agacggagta gacggccgcc agcagcagcg
    10021 ggtagccggg tgggtgcttg atgatgctgg cctggagcgg ccactcgtcg ccgcgcacga
    10081 agcgcgtcac gtcgccctcg tagagcagca gcgcgtgctc cttgtactcc acggccatgc
    10141 cctcgtagat gaaagcgtgc cggctgtcct gccagtggag cgcgcggacg ccgagcgcga
    10201 cgaggaagac gacggcgcag aggacgaaga gacgcgcgcg ccgcacgggc cggcgttgcg
    10261 cgctcgtctc gtgtctggtc tgtgcgtgtg cttcgtcggt ggtcatcgga ttcgccttgc
    10321 gtcctcccgc gcgtggggcc gaaggacggc cgcagagtat agcaagctct acaacccggc
    10381 gcaggtatcg cgcacgcgct aaatgagcac gttgtgtaac gccggcgtcg gtgcttcacc
    10441 ttcgttgggc tttgagatag agtgccctgg tttatgaacg tcgaagagtt gaatctggtg
    10501 aacacggcga gcgcgagtcc ggcggatgag acggaggtgc gcggggcgga cgctgcccgc
    10561 ttgcgttacc ggcgcgagcg cgccgcgctg tgggacgcgc aggcccgccg gatggacacg
    10621 tggcggggct ggggcggcgc ttaccaccgg cgtctggagg agctttaccg cttcttcgtg
    10681 ccgccgggca gccgcgtgct cgacatcggc tgcgcgcgcg gcgacctgct ggccgcgctc
    10741 gaaccttcgg tcggcgtcgg cgtggacttc tccaccgaga tggtcgagcg cgcgcgcaag
    10801 cgtcacccgc acctgcgttt tctacaggcg gacgcacacg agttggagat agaaggtgag
    10861 ttcgacttca tcatcctctc cgacgtggtc aacgatttgt gggacgtgca ggaggtcttc
    10921 cgtcgcgtgg ggcagttgtc ggggccgcgc acgcgcgtcg tcctcaactt ctacagccgc
    10981 ctctgggagc tgccgctcgc gctcgcgcag cgcgcgggcg cggccacgcc gcaactgccg
    11041 cagaactggc tcacggtcga agacgtgtcg ggcctgctct cactcgccga cttcgaggtc
    11101 atccgccgcc gcgacgagat actgctgccg ctgcccgtcc cgccgctttc tactctctgc
    11161 aaccgctacc tcgtcaagct ctggccgttt aatgtcttcg cgctgactca cgtcctcgtc
    11221 gcgcggcccg cgggccgtgt cgagcagcag ccggcggcgg cggcggcggt gccgcgcgtc
    11281 tccgtcgtcg tccccgcgcg caacgaggcc ggcaacatcg ccgacatctt ccggcgcacg
    11341 cccgagatgg ggagcgagac cgagctgatc ttcgtcgagg gcggctcgac cgacgacacc
    11401 tacgagacca tcgagcgcga gatggccgca caccccacgc ggagcgcgcg gctcctgcgg
    11461 cagaccggac gcggcaaggg cgacgccgtt cgcacggggt tcgcgcacgc caccggcgac
    11521 gtgctgatga ttctcgactc cgacctcacc gtcccgccgg aagacttgcc gcgcttcctc
    11581 gaagtgctca cgtcgggcaa ggccgacttc gccaacggct cgcgcctcgt ctacccgatg
    11641 gagcgcgagg ccatgcgctt cctcaacctc gtcggcaaca agttcttcag cctcgccttc
    11701 tcgtggctcc tcggtcagcc cgtcaaggac acgctctgcg ggacgaaggt cttgcgccgg
    11761 gacgactacg agcgcatcgc cgcgggccgc gcctacttcg gcgacttcga ccccttcggc
    11821 gacttcgacc tcctcttcgg agcctcgaag cagaacctca agctcctcga cgttcccgtc
    11881 cgctatcgcg accgcaccta cggcgcgacc aacatcagcc gctggagcca cggctggctg
    11941 cttttgcgga tggtcgcctt cgccgcccgc cgcatcaagt tcatttgaga gaagtgagaa
    12001 gtggacaggt aaaaaagtag cgggccggct gcgccctcgc tgcaccgccg gcccgctctc
    12061 gaagcgactc tcacccgaag ggagagaagc aacgagaaac agactctatc accagtcggg
    12121 ccgggctggc aagctctgta cggtaacgca cggccggcaa gcaaaagcga agggcgcgtc
    12181 ttcaaaagag gacgcgccct tcggctcatt gcggacggcg tgtgcggccc cgacgcccgt
    12241 acttttatta ccgtatcggc ggcgactcag ccccggccgg cacgatccag agctgtatgg
    12301 tgagggcgtc gcgctggtca ccgaggcgcg tgacgacgcg gcccggctcg atgccgcgcg
    12361 tcatcgtcag gtagtcgcgg atgcggtcgg cgcggcgt
//
LOCUS       DASVES010000343         4629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_601020, whole genome shotgun sequence.
ACCESSION   DASVES010000343 DASVES010000000
VERSION     DASVES010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601020"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VF240_14000"
     CDS             complement(<1..1082)
                     /locus_tag="VF240_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176372.1"
                     /translation="MNYEGEQLRGLIRDARRRRRLLLALRGAAVCLCVAAATLLLTGW
                     AAHRYRHAEAALLALRLGALFAFVVAAYFSLVRPLARRITDARLARLIEERTPGADDR
                     LVAAVEYSDASEGRRASRAIVGRLRLDADRTAAGVNLDAVVSRRRLAAYGAASLACLL
                     FVAGVLKWGPRAVSEGVAQLVTPSGLAAAAPDMSIAVKPGTARVPKGSDQEITATLAG
                     FDAEAVSFYSRAAGAGDTEWRGEPMEPAKAAAEFQFRIFNIQEATEYFVESNGVRSEV
                     FRLEVVDLPFVKQLDLTLVFPAHARMPAKTVEDGGDIAALKGTTATITARLSARARAA
                     RLVLKDGRRIEMKATGSDFVGALAVSG"
     gene            complement(1242..3302)
                     /locus_tag="VF240_14005"
     CDS             complement(1242..3302)
                     /locus_tag="VF240_14005"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX7176373.1"
                     /translation="MSFLNPLFLLGLAAVAAPILVHLVRRTRARRVEFPALVFVRQIP
                     QRTIRRRTLHNLLLLLLRCLAVLLVVFAFTRPFFSGAGASAETETTRATVFLIDSSLS
                     MRHADHFSEAVRRADTILGELPEDERVALVRFGEGYEVLSEFTAEKHRVRAALAGLGA
                     GFEGTDYEQALRGAESLFTDLRAGGAKRVFLISDFQAAGWQAARATFRLRDDIRLALI
                     DVGGKETTPNVAVTNVEARGVVYGQKYVDKVAVHLSNFGDVARERVGVDLQVNDQTVE
                     KREISLAARDSRVVEFTGFNLGEGPNRCAVRLRADDFEHDNQFYFTLRGETPAKAVII
                     DSAARGLGDSFYLQSALSAGEGLPFAFSVKSAGALDPADVAGYSLVVLNDAGNISGAL
                     AEAVVKFVESGGPVIVATGPNTRAESFNRALERVAPVRLTDAVQLQRGESAAVTDVKT
                     AHPVFEIFRGGATLSSARVFGYHRSEPRERAVVLARFEDGSPALVESSAGAAGRVLLF
                     TSTLGMNWNDLPLTPLYLPLVQQMVRYLGEREAVAWHRLGQTFTVARASGGEPPAIDT
                     PSEERLKTGTRTPDGDLLVTGREPGFYRLRYEGRPDFAAVNVDGREGDFAKLNTDEFL
                     AGVTGGDAGASAEAEADADARVSGAEIEARQRVWWPLLVAALLLFAAEAVLARRTKMV
                     RMIG"
     gene            complement(3403..4368)
                     /locus_tag="VF240_14010"
     CDS             complement(3403..4368)
                     /locus_tag="VF240_14010"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7176374.1"
                     /translation="MWWSRNKKNQSEAAAETSPAPAFAPGPARFLDPEVLARIGSLEL
                     LARAVVEGFISGLHRSPYSGFSTEFKEYRQYNPGDDLRYLDWRLLGRTDRYFIKKYRA
                     DTNAQCHLLVDTSASMRYASAGAVTKLQYAQFLAAALAHLVARQQDSVGLLAFGERVH
                     THVPARNRTGHLRTIYGQLSTLEAGGESRISAALHETADRLTRRGIVVVVSDFYDQPE
                     RLADAFQHLRFRGHDLVAFHLLDRNEMEFDFDDAVLLLEDSETAQQMPVLPDVIAGGY
                     RDRIAAHVDELRRCCAANRVDYELITTDKPLDFALFSFLSRRAGK"
     gene            complement(4415..>4629)
                     /locus_tag="VF240_14015"
     CDS             complement(4415..>4629)
                     /locus_tag="VF240_14015"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7176375.1"
                     /translation="RASQFLVLGGKARAILRGRYNVSCEDVRAVAPAVLRHRILLNFQ
                     AEAEKIDSDEVIRRLVEAVPEPKSGL"
BASE COUNT          788 a         1557 c         1561 g          723 t
ORIGIN      
        1 cccgacactg cgagtgcccc gacgaagtcg gagcctgtgg ctttcatctc gatgcggcgg
       61 ccgtctttca ggacgagccg cgcggcgcgc gcgcgcgcag agagtcgcgc cgtgatggtc
      121 gccgtcgtgc ccttgagcgc ggcgatgtcg ccgccgtcct ccacggtctt cgccggcatg
      181 cgcgcgtgtg ccgggaagac gagcgtgagg tcgagctgct tgacgaaggg cagatcgacg
      241 acttcgaggc ggaagacctc tgagcggaca ccattcgact cgacgaaata ctcggtcgcc
      301 tcctgaatgt tgaagatgcg gaactgaaac tcggccgccg ccttcgccgg ctccatcggc
      361 tcgccgcgcc actcggtgtc gccggccccg gccgcgcgcg agtagaagct gacggcctcc
      421 gcgtcgaagc ccgcgagcgt cgccgttatc tcctgatccg aacccttcgg cacgcgcgcc
      481 gtgccgggtt tgaccgcaat gctcatgtcg ggcgcggcgg cggcgaggcc cgacggcgtg
      541 acgagttgcg ccacgccttc cgagaccgcg cgcggccccc acttcaacac gccggcgacg
      601 aacaacaggc acgcaagcga cgccgccccg taggccgcga gccggcgtcg gctgaccacg
      661 gcgtcgaggt tgacgcccgc cgccgtgcgg tcggcgtcca gcctgagccg cccgacgatg
      721 gcccgcgagg cgcgtcgccc ttctgacgcg tcggagtatt cgaccgcggc gaccagccgg
      781 tcgtccgcgc cgggcgtgcg ttcttcaatc aggcgcgcga gccgcgcgtc ggtgatgcgc
      841 cgcgcgagcg gcctcacgag cgagaagtat gcggccacga cgaaggcgaa gagggcgccc
      901 agacgcagcg cgagcagcgc ggcctcggcg tgtcggtagc ggtgtgccgc ccagcccgtc
      961 aacagcaagg tggccgccgc cacgcacagg cagacggccg cgcccctcaa cgcaagcagc
     1021 aagcgccgtc gccgccgcgc gtcgcggatg agaccccgca gttgttcacc ctcgtaattc
     1081 atcgaatgac ctcgcagtat aaacacgagg gaagaattaa catggatgga caggatgcat
     1141 aggatgaaat catgttattg gatttgagat ttgaaatctg aaatctctca tcctgcttat
     1201 cctgtccatc catgttaatg attcttccgt aaaacttacc ttcatccaat catcctcacc
     1261 atcttcgtcc gccgcgcgag tacggcctcg gcggcaaaga gtaggagcgc cgcgacgagc
     1321 agcggccacc agacgcgttg ccgcgcctcg atctccgcgc cgctcacgcg cgcgtccgcg
     1381 tcggcctccg cctcggcgga cgcgccggcg tcgccgcccg tcacaccggc gaggaactcg
     1441 tccgtgttga gcttggcgaa gtcgccctcg cgtccgtcca cgttgacggc cgcgaagtcg
     1501 ggccgccctt cgtaacgcag gcggtagaac cccggctcgc gccccgtcac gagcagatcg
     1561 ccgtcgggcg tgcgcgtgcc cgtcttcaga cgctcttccg aaggcgtgtc aattgcgggc
     1621 ggctcgcccc ccgacgcgcg cgcgaccgtg aaggtctgtc cgagccggtg ccacgccacg
     1681 gcctcgcgct cgccgaggta gcgcaccatc tgctggacga gcggcaggta gagcggcgtc
     1741 agcggcagat cattccagtt catgccgagg gtggaagtga aaagcagcac gcgccccgcc
     1801 gcccccgcgc tcgactcaac gagagcgggg cttccgtcct cgaagcgcgc cagcacgacg
     1861 gccctctcgc gcggctccga gcggtggtag ccgaaaacgc gcgccgagga gagcgtcgcg
     1921 ccgccccgga agatttcaaa gaccggatgc gccgtcttca cgtccgtgac ggccgccgac
     1981 tcgccgcgct gaagttggac ggcatcagtg agccgcaccg gcgcgactct ctcaagcgcc
     2041 cggttgaaac tctccgcgcg cgtgttcggc cccgtcgcca cgattaccgg gccgcccgac
     2101 tcgacgaact ttacgaccgc ctccgcgagc gcgccggaga tattccctgc gtcgttcaag
     2161 acgacgagcg agtatccggc cacgtccgcc gggtcgagcg cgcccgcgct cttcacgctg
     2221 aaggcgaagg gcaggccctc gcccgccgag agcgcgcttt gcagatagaa gctgtcgcct
     2281 aagccccgcg cggcgctgtc gatgatgacg gccttcgccg gcgtctcgcc gcgcagggtg
     2341 aagtagaact gattgtcgtg ctcgaagtcg tcggcgcgca ggcgcaccgc gcagcggttc
     2401 ggcccctcgc ccagattgaa gccggtgaac tcgacgaccc gcgagtcgcg cgccgcgagg
     2461 ctgatctctc ttttctcgac cgtctggtcg ttgacctgta aatccacgcc gacgcgctcg
     2521 cgcgccacgt cgccgaagtt gctcaagtgg acggcgacct tgtcaacgta cttctgtccg
     2581 tagacgacgc cgcgcgcctc gacgttcgtg acggcgacgt tcggcgtcgt ctctttgccg
     2641 cccacgtcga tgagcgcgag gcggatgtcg tctctcagcc ggaaggtggc gcgcgcggcc
     2701 tgccagccgg cggcctgaaa gtcggagatc aggaagacgc gcttcgcccc accggcgcgc
     2761 aggtctgtga acaacgactc ggccccacgc aacgcctgct cgtaatccgt cccctcgaag
     2821 cccgcgccga ggccggcgag cgcggcgcgc acgcggtgct tctcggccgt gaactcgctc
     2881 aagacctcgt agccctcgcc gaatctcacg agcgcgaccc gctcgtcttc cggcagctcg
     2941 ccgaggatgg tgtcggcgcg gcgcacggcc tccgagaaat gatcggcgtg gcgcatgctg
     3001 agcgaagagt cgatgaggaa aacggtggcg cgcgtcgtct cggtctcggc ggacgcgccg
     3061 gcgccgctga agaaggggcg cgtgaaggcg aagacgacga gcagcacggc gagacagcgc
     3121 agcagcagga gcaacaggtt gtgcagcgtc cgccggcgga tggtgcgctg cgggatttgc
     3181 cgcacgaaga cgagcgccgg aaattccacg cgccgcgccc gcgtgcgccg cacgagatgc
     3241 acgagaatgg gcgcggccac cgccgccagc cccaacagga ataatggatt cagaaacgac
     3301 atctgttagc tgagtgatga gtgataagtg ataagtgata agaaggacaa accgccttct
     3361 ctttcttatc acttatcact catcacttat cacttcctac gttcacttcc ccgcgcgccg
     3421 cgagaggaag gagaagagcg cgaagtcgag cggcttatcc gtcgtaatca actcgtagtc
     3481 aacgcggttg gcggcgcagc accggcgcag ctcatcgacg tgcgcggcga tgcggtcgcg
     3541 gtagccgccg gcgattacgt ccggcagcac gggcatctgc tgggccgtct cggagtcttc
     3601 gaggagcagc accgcgtcgt cgaaatcgaa ctccatctcg ttgcggtcga ggaggtggaa
     3661 ggcgacgagg tcgtggccgc gaaagcggag gtgctggaag gcgtcggcga gccgctccgg
     3721 ctggtcgtag aagtcggaga cgacgacgac gatgccgcgg cgcgttagcc ggtcggccgt
     3781 ctcgtggagc gccgcgctga tgcgcgactc gccgcccgct tcgagggtcg agagctggcc
     3841 gtagatggtg cggaggtggc cggtgcggtt gcgcgccggg acgtgcgtgt ggacgcgctc
     3901 gccgaaggcg aggaggccga ccgagtcctg ctggcgcgcg acgaggtggg cgagcgcggc
     3961 ggcgagaaac tgcgcgtact gtagcttcgt cacggccccc gcgctcgcgt agcgcatcga
     4021 ggcgctggtg tctacgagca ggtgacactg ggcgttggtg tcggcgcgat acttcttgat
     4081 gaagtagcgg tcggtgcggc ccagcagtcg ccagtcgagg tagcgcaaat catcgccggg
     4141 gttgtactgg cgatactcct tgaactcggt cgagaagccc gagtacggcg agcggtgcag
     4201 ccccgagatg aagccctcga cgacggcgcg cgccagaagc tccagtgagc cgatgcgcgc
     4261 cagcacctcg gggtcgagga agcgcgccgg gccgggcgca aaggccgggg ccggacttgt
     4321 ctcggcggcc gcctctgact gattcttttt atttcgactc caccacatga cttttacttg
     4381 tgtaacgaat cctgcactca tctaaaccac aaccctaaag ccccgacttc ggctccggca
     4441 cggcttcgac gaggcgtcgg atgacctcgt cggaatcgat cttctccgcc tccgcttgga
     4501 agttcagaag gatgcgatga cgaaggacgg cgggcgcgac ggcgcgcacg tcctcgcacg
     4561 agacgttgta gcggccgcgc aggatggcgc gcgccttgcc gccgaggacg aggaactgcg
     4621 aagcgcgca
//
LOCUS       DASVES010000344        15294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1630254, whole genome shotgun sequence.
ACCESSION   DASVES010000344 DASVES010000000
VERSION     DASVES010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630254"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF240_14020"
     CDS             complement(<1..279)
                     /locus_tag="VF240_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176376.1"
                     /translation="MPSSEAEKQSRPQPERASAATERTPRPGGPWVEALRRSDVSRLW
                     GELQRLVSNHPLVRASHSAGLLVEEGDRGSAYIDLTQELFVTLLAKSRF"
     gene            976..1308
                     /locus_tag="VF240_14025"
     CDS             976..1308
                     /locus_tag="VF240_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176377.1"
                     /translation="MNRVSTARFRKLRTCPSAEVLIQYGQAGLACDTEERVAHHLTSC
                     DFCGAEMQLLSKHWRRDFPALKIAGDMSLSLRRLAEDLLTTLPDLNRARFAETICDMD
                     RLTVSDAV"
     gene            complement(1455..3302)
                     /locus_tag="VF240_14030"
     CDS             complement(1455..3302)
                     /locus_tag="VF240_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7176378.1"
                     /translation="MNEPTHLHLPTPATIPEMCLTALRAHMKRDAVNLKRDGLWIHLP
                     SEAIETRIRAVALGLSGMGVRKGDRVALLSENRPDWSIVDLAILSLGAVNVPIYTTQA
                     PEQVRYILEDSGAAVLFVSGRKVFRHAQAEIEAVAGLQNIVFFDADGIEPGGRVMLLT
                     TLEQAGAELDRANPEGFDCAWRGVGSEDLATIIYTSGTTGEPKGVMLTHSNFVSNVLA
                     ISQSLPISADDVSLSVLPLSHIFERTVFYVFCWCGVSVHYAPSFDVVAEYLREVRPTI
                     MTAVPRLFERVYHRIVKKGTSEGGLKEKLFRWSLGVGQRYAELRDKGEPAPPLLELQH
                     EAASRLVFSKWREGVGGRLRYFVSGGASLSPALSYAFFGAGINILQGYGMTETCIVSA
                     NRPDDNRVGSVGVPFPGVDVAIDPEGEILVRGPNVMLGYYNHPQETEAAFTPHGWFKT
                     GDVGRLDERGHLFITDRKKELFKLSNGKYVAPQLLESLIKQSELVSQVVVVGAGRKQP
                     AALVVPDWEALAAAAGDGVEGKRQKREEWSRDPAAVKLVQREVAQLTSSLADYERVRR
                     VALLPEEFTIDGGEMTPTLKIKRRVVDEKYGALIDRLYDGGAGAAAKDE"
     gene            complement(3363..4325)
                     /locus_tag="VF240_14035"
     CDS             complement(3363..4325)
                     /locus_tag="VF240_14035"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX7176379.1"
                     /translation="MRILVTGGAGFIGSHLCERLLDEGHEVLCLDNFFTGRRANVLHL
                     LDHKSFELIRHDVIEPILLEVDQVYNLACPASPIHYQYNPVKTVKTSVMGAINMLGLA
                     KRVRARILQASTSEVYGDPEVHPQPESYWGSVNPIGPRSCYDESKRLAETLMMDYHRQ
                     NRVDTRIIRIFNTYGPRMLENDGRVVSNFIVQALRGEELTIYGDGSQTRSFCYVDDLV
                     DGMIRLMNVEPGDAPPGIDVHDPVNVGNPGEFTMRQLAEEVGRSVGVEVRLRHLPLPQ
                     DDPRQRRPDITRAQQLLGWQPTVPLREGLKRTVAYFSERVKKPR"
     gene            complement(4435..6018)
                     /locus_tag="VF240_14040"
     CDS             complement(4435..6018)
                     /locus_tag="VF240_14040"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7176380.1"
                     /translation="MTTSISNVSAEAPRPARRRRKDSERALTTRPKGAPNTERTAFSR
                     YIVLALCLALVLSTLAFGTVHNWSLAVFQGTAGVVVALWAADAWRARRLRFSKNFLQL
                     PLAGLFLLGLFQLLPLAGAPAPGDPAAALATAPARTLSFDPYATRLVLIQLAGLFVYF
                     AAALAFIDSARRLRLIVRTVIVFGFALALFGLIQFFVSPDRIFGLRETRQALGFGPFI
                     NRHHFANYMVMAAGLPLGLLFAGAIERERRLLYLFSAVLMGIALMMTNSRGGMLALVA
                     EVFFLVVVSGLVRRPAHHGGDGGEDAQEHTRARALLLRAAAGLGIVVALIAGVVFFGG
                     EDSLNRLLGTVNSEDPTTGRLHFWRGTVEIIRAHPWLGAGLGAFSAVYPRYDTSNGSI
                     YRLEQAHNDYLQLLSDGGIVAFALGLLFVAGLFAMALRRMQSTDAFRRGVALGALSGC
                     FAALVHSFFDFPLHTTSNGLLFLTLAALATAGGRVEQTRRRRKRKQQGRRETPAGAGE
                     EVVGADGARARASEGAATA"
     gene            complement(5948..7345)
                     /locus_tag="VF240_14045"
     CDS             complement(5948..7345)
                     /locus_tag="VF240_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176381.1"
                     /translation="MPDKDQIKTVGVEALWRRVLVVLPALAVLFASWTAARWCMGSTL
                     ASNPPAVESVIAAARVRPNDPQVQEALAQIGGILHAAERWAPDDPQVHYTLGVFARKS
                     FAPEELREVLVRYERAVSLAPHDHRLWMELGRARGQAGDEEGGALALRHAVSLAPHYS
                     MPRWYLGNLLLRAGREPEAFAELRRAAEIDPTLHTQIFTTAWSYFGGDVRAVGAAVGD
                     SYEARARLVQHLLTQKRLDEALILWGTLKTEERREQAASGASLMNALIEAGRYRKALE
                     VYGEVQPAAAQDVAVGKLFNGGFERDVTANSGNPFDWIVRPVPQAQMNIDARNAREGR
                     RSLRLEFSAPSEFTLGQFSQLVLVEPRARYRLSYAVRTEEVKSAATLVVQVTDAAGVA
                     FAGGAAAPAGTSDWQQVIVEFTAPAAEAVTLRLVRVGCSDGACPIFGKVWYDDFNLQR
                     VGGSAAAGAPAPKGQ"
     gene            complement(7448..9271)
                     /locus_tag="VF240_14050"
     CDS             complement(7448..9271)
                     /locus_tag="VF240_14050"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176382.1"
                     /translation="MLFTTEQLLAVAAALALALVLTPLVRAYARSRKMVAQPKPDRWH
                     RAPTAMLGGLAVFLTVIIIYAAFVPHTPQGLAVMAASSFLFLVGLIDDLLHIKPYQKL
                     IGQMMGAAMLIYFGAATVGLGGLTLPWTASVPVNMAITIFWLIGITNAVNLLDNMDGL
                     AAGIAAIASAFLAVNFIGNGQAIEALMLAVFAAALAGFLVYNSNPATIFMGDCGSMFI
                     GFFLASTSLLAASGGRSRSFLPVLAVPVLTLFIPIFDTTFVTLIRKLSGRGVSQGGRD
                     HTSHRLVALGMSERKAVWMLYGFAALSGLLALFVREVAFDVSLAAVLGFTVVLTLVGV
                     YLAGVKVYDEEEVRAARDKPLVAFLVDLSYKRRVFEVFLDAALIILSFYASYLLLYGP
                     VNEIGATELFLRTVPVLVFVKMGAFLVAGVYRGIWRYVSLDNLVVYVKAVVAGSVASV
                     LVLLFAFRFQDYSRSVFVLDAIILFVLLAGSRVAFRMLRKVMPMGSSRTGRRVLIYGA
                     GDAGELLLREILNNPELNYAPVGFADDDRHKMGKVIHGLRVFGGNGKLRGICETLHVE
                     EVLISSLKLSEDRVRDIVRTCSDARVSVKRMRIQIDDLSGE"
     gene            complement(9539..10525)
                     /locus_tag="VF240_14055"
     CDS             complement(9539..10525)
                     /locus_tag="VF240_14055"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7176383.1"
                     /translation="MRVLITGGAGFIGSHLTDAYMARGDEVFCIDDLSTGSFDNIAHL
                     KANPRYHYTIDSVNNRRLMAELVDQCDVIFHLAAAVGVKLIVESPVRTIETNVHCTEV
                     VLAQAAKKKKKVLVASTSEVYGLSSEVPFREDGNLVMGATTKGRWSYACSKAIDEFLA
                     LAYWREQRLPTVVVRLFNTVGPRQTGQYGMVIPTFVKQALAGTPITIYGDGKQTRCFG
                     YVGDVVGALVKLMETEEAVGQVFNIGSNEEVSILELAERVKSLVGSKSEIVFVPYDEA
                     YEEGFEDMPRRVPDTSKIERAVGFRPTVTLDEILRTVIDYYGRRPAAAGGQA"
     gene            complement(10542..11888)
                     /locus_tag="VF240_14060"
     CDS             complement(10542..11888)
                     /locus_tag="VF240_14060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7176384.1"
                     /translation="MKVVRIIARLNVGGPARHVAWLSAGLVERGVETVLVAGTVPPGE
                     DDMGYFSEALGVRPHIIPEMSREVSPKDAVTVWKLYRLLVRERPDVVHTHTAKAGTVG
                     RVAGLLYRWLTPSALWGRPRSVRFVHTYHGHVFHSYYGPRKTRLFLAIERALARVATD
                     RVVVISPRQLEEIGGHFRVGRPAQFAVIPLGLDMRAFADWAERRPRLRRELGAGDDEI
                     LVGIVGRLTAIKNHALFLAAAEHYKRHAAREAETEGRTLRRVRFVVVGDGLLRRQLEA
                     QAASLGIIDDVIFLGTRDDPENFYPALDVVALTSLNEGTPLTLVEAMANARPVIATAV
                     GGVPDLLGGLDPALLQRRPRGWQVCERGVLVEECDAESFSRALDYLIENEELRLGMGA
                     RGLEFVARQYSVERLLADIHRLYEELIDPAESAAQQGARAAGPGTDITRARRGRAS"
     gene            complement(12061..12813)
                     /locus_tag="VF240_14065"
     CDS             complement(12061..12813)
                     /locus_tag="VF240_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7176385.1"
                     /translation="MKGVVLAGGLGTRLRPLTSVTNKHLLPVYDKPMIYYPIQTLVNA
                     GVRDIMIVTGGNSAGDFLKLLKNGKDFGLKHLNYTYQEGEGGIAAALDLCEDFSDGEP
                     ICVVLGDNIIEGSIGRAVRSYRHQGGGAKILLKKVSDPQRFGVPELDGRRVVRIEEKP
                     AEPKSDYAVIGIYMYDAEVFRIIKTLKPSGRGELEITDVNNAYIGRDEMSWDELEGWW
                     TDAGTFESLLHASNLVAETGANKMEDVKAISG"
     gene            complement(12946..14832)
                     /gene="asnB"
                     /locus_tag="VF240_14070"
     CDS             complement(12946..14832)
                     /gene="asnB"
                     /locus_tag="VF240_14070"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7176386.1"
                     /translation="MCGITGIVRREGRQVDEALLASMCEAIRHRGPDEDGFYHGERTG
                     LAMRRLSIIDLAGGQQPIHNADRSAWIVFNGEIYNYKELRKQLADLGHEFYTDSDTEA
                     VVHAYTRYGSDAPKHLRGMFAFAIWDERKQELFLARDRVGKKPLLYARTPSGDFVFGS
                     EFQALLQHPEVSRDIDHEAIDRYLSFMCVPAPLTAYRMIRKLEPGHSLRLTRDGEIKI
                     ERYWMPDFNRKEKWTEEEASERAVELLRDAVRVRLMSEVPLGAFLSGGIDSSAVVALM
                     SEESSAPVKTFSIGFDEQDFSELHHARRVAELVGADHHEFIVRPDAMEVLPTLVEHYG
                     EPYADSSAIPTYYVARETRRHVTVALNGDGGDECFAGYDRYAAMRLAETYRRVPAALR
                     GAFIEPALSLMPSSEVRRSYLGRVKRFVRAASLPSAERYAQWVSVFDDAAKQMLYTEG
                     FRAETRMQRASDLIAPWFTMANGAGVVDAALLADTMTYLPNDLLVKVDIASMAVSLEA
                     RSPFLDHHVIEFAASLPEHLKLRGRTTKYLLKRALRGLVPEENLTRGKMGFGVPIGHW
                     FRGRMEPFLRETLLSEKSLGRGFFKPERVRGLVEEHTVRGLDRSHHLWILLMLELWFQ
                     RFID"
     gene            complement(14836..>15294)
                     /locus_tag="VF240_14075"
     CDS             complement(14836..>15294)
                     /locus_tag="VF240_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176387.1"
                     /translation="AITAIPVGMIYLLFAPFPWQLVNLRQSLTLPEMLVWWGAFPFLV
                     VGLWFTVRYRMRQALPILVFTTMLTLAYSVFQGNVGTAYRQRSQLLVFYFIFASVGLV
                     IARETKEDKQREARQARLLAQNATALALARRKQAQEWQSIADRLSEKIGF"
BASE COUNT         2645 a         4883 c         5270 g         2496 t
ORIGIN      
        1 gaagcgcgac ttggcgagga gggtgacgaa caactcctgc gtcagatcga tgtaggcgct
       61 gccgcggtcg ccctcctcga cgagcagccc ggcgctgtgc gaggcgcgca ccagcgggtg
      121 gttgctcacg aggcgctgca attcccccca cagccggctc acgtccgagc gccgcagcgc
      181 ctcgacccac ggcccgcccg ggcgcggcgt gcgctccgtc gcagcagagg cgcgctccgg
      241 ctggggacgt gactgttttt cggcttcgga actgggcatg ggattgttca agtaatgatt
      301 attgtcggcg ggggttgcgg tcatgttaat cgcaatcact taccggagca agccttatgg
      361 aggacggcga gcaggttttc gactgggaag ttgcccgatg aggagcaagg gcggcggcgt
      421 tattgacctt cgaccacggt cctggcagac cgcgtggggc tacttagtac tcgaaggcgc
      481 cgttaccagg aagagtttgc gctccgtaag tcccttttgg attttgacct tgccgggatt
      541 tccgcgacct gtgaccgctg aaggggaaac gggtgcggtc atcgtgcagg ggtccgaggg
      601 gcgctcgtgg cggcccttcg gaggacggaa tggatgttag cagcaaaccg gaaattgggc
      661 aacatctttt tacaccgacc tcaacctgtt gattgccaaa cacaaaaggg gggtaaaagg
      721 tgtccgccgt cgcccccgaa gattggaaaa aaggggggat tgagttttta ctttgatgat
      781 tcaagcgaag cgcccgatga caaaaggata gacggcttga acagcggccc tctcgccgca
      841 cacttgccct agcctttatt cgtgctgtgc ctctccaccg ccgggcgctt gcgatgccgg
      901 ccccgctctc tcgccccgaa gagagcagtg tcgggcagtc ggcgttagct atgcaagatc
      961 atggaaggag cgaccatgaa ccgtgttagc acagccagat tccgtaaact tcggacgtgc
     1021 ccgtccgccg aggtgctgat ccagtacggg caggcggggc tcgcgtgcga caccgaggag
     1081 cgtgtggccc accacctcac ctcgtgcgac ttctgcgggg ccgagatgca gctactctcc
     1141 aaacactggc ggcgcgattt ccccgctctc aagatcgcgg gcgatatgtc tctgagtttg
     1201 cgccgtctgg ccgaagacct gctgacgacc ctgccggact taaaccgcgc gcggttcgcc
     1261 gagacgatct gcgacatgga ccgcctgacg gtgtcggacg ccgtctagtc agaagatttc
     1321 aggcggcctc acgcgaagac ccctcgcccg aaattgccgc tctgccctct ctcttagccc
     1381 caataaagct cacatcttag accccgaacg aaacgtcttc gagttgcgcc gcgtggcgcg
     1441 ggcgccgccc gcgcctactc gtctttcgcc gccgcgcccg cgccgccgtc gtagaggcgg
     1501 tcgataagcg cgccgtactt ctcatcgacc acgcgcctct tgatcttgag cgtcggcgtc
     1561 atctccccgc cgtcgatggt gaactcttcg gggaggagcg cgacgcggcg gacgcgctcg
     1621 tagtcggcga ggctggaagt gagctgcgcc acctcgcgct gcaccagctt gacggcggcc
     1681 ggatcgcggc tccactcctc cctcttttgt cgcttaccct ccacgccgtc gccggcagcg
     1741 gcggcgagcg cctcccagtc gggcacgacg agcgcggccg gctgcttgcg ccccgcgccc
     1801 accaccacca cctgactcac caactcgctc tgcttaatca gactctccag aagctgcggc
     1861 gcgacgtact tgccgttcga gagcttgaac agctccttct tgcggtcggt gataaagagg
     1921 tggccccgct cgtcgaggcg gccaacgtcg cccgtcttga accagccgtg cggcgtgaaa
     1981 gccgcttcgg tctcctgcgg gtggttgtag tagccgagca tgacgttcgg cccacgcacg
     2041 aggatttcgc cctcggggtc gatggccacg tcaacgccgg ggaacggcac gccgacggag
     2101 ccgacgcggt tgtcgtcggg gcgattggcc gagacgatgc aggtctcggt catgccgtag
     2161 ccttgcagga tgttgatgcc cgcgccgaag aaggcgtagg agagcgcggg cgacagcgac
     2221 gcgccgcccg agacgaagta gcgcaggcgc ccgcccacgc cctcgcgcca cttggagaag
     2281 accagccggc tcgcggcctc gtgttgcagt tcgaggagcg gcggcgccgg ctcgcccttg
     2341 tcgcgcagct cggcgtagcg ctgtcccaca ccgagcgacc agcggaagag tttctccttg
     2401 aggccgccct cggacgtgcc cttcttgacg atgcggtggt agacgcgctc gaagaggcgg
     2461 ggcacggccg tcatgatggt cgggcgcact tcgcgcaggt actcggccac cacgtcgaag
     2521 gacggcgcgt agtggacgga gacgccgcac cagcagaaga cgtagaagac cgtgcgctcg
     2581 aagatgtgcg agagcggcag aacggagagc gaaacgtcgt cggccgagat gggcaggctt
     2641 tgcgagatgg cgagcacgtt cgagacgaag ttcgagtgcg tgagcatcac gcccttcggc
     2701 tcgcccgtcg tccccgacgt gtagatgatc gtcgccaaat cctcgctgcc gacgccgcgc
     2761 caagcgcagt cgaagccctc ggggttggcg cggtcaagct cggcgccggc ctgctccagc
     2821 gtggtcaaaa gcatcacgcg gccgcccggc tcgatgccgt ccgcgtcgaa gaagacgatg
     2881 ttctggaggc cggcgaccgc ctcgatctcg gcctgcgcgt ggcggaagac cttgcggccc
     2941 gagacgaaga gcacggccgc gcccgagtct tcgaggatgt agcgcacctg ttcgggggcc
     3001 tgcgtggtgt agatggggac gttgacggcg ccgaggctga ggatggccag atcgacaatc
     3061 gaccagtcgg gccggttctc cgagagcagc gccacgcggt cgcccttgcg cacccccatg
     3121 cctgagaggc ccagcgccac ggcgcggatg cgcgtctcaa tcgcctccga cggcaggtgt
     3181 atccacagcc cgtcgcgctt gaggttaacg gcgtcgcgct tcatgtgcgc gcgcagggcc
     3241 gtgagacaca tctcggggat ggtcgccggc gtgggcaggt gcaggtgtgt aggctcgttc
     3301 atgagtgata agtgataagt gaaaagtgat aagaaggata aggaagctgt ctttcttatc
     3361 acttatcgcg gctttttaac tctttcggag aagtaggcga ccgtccgttt gagtccttcg
     3421 cgcaggggga cggtgggttg ccagccgagt agctgctggg cgcgcgtgat gtcggggcgg
     3481 cgctggcgtg ggtcgtcctg tgggagcggc aggtgacgca ggcgcacctc gacgccgacg
     3541 gagcggccga cctcctcggc gagctgcctc atggtgaact cgccgggatt gccgacgttg
     3601 acggggtcgt gtacgtcgat gccgggcggc gcgtcgcccg gctcgacgtt catcaggcga
     3661 atcatgccat cgacgaggtc gtccacgtag cagaaggagc gggtctgcga gccgtcgccg
     3721 tagatggtca gctcctcgcc gcgcagcgcc tgcacgatga agttggagac gacgcggccg
     3781 tcgttttcca gcatccgggg gccataggtg ttgaagatgc gaatgatgcg cgtgtccacg
     3841 cggttctgcc ggtggtagtc catcatcagg gtctcggcga ggcgcttcga ctcgtcgtag
     3901 cacgagcgcg ggccgatggg gttgacgctc ccccagtagc tctcgggctg cgggtgaact
     3961 tcggggtcgc cgtagacttc cgacgttgac gcctgaagga tgcgcgcgcg cacgcgcttg
     4021 gcgaggccga gcatgttgat ggcgcccatc acgctggtct tgacggtctt gacggggttg
     4081 tactggtaat ggatgggcga ggcggggcag gcgagattgt agacctgatc gacttcaagg
     4141 agtatcggct cgatgacatc gtggcggatc agctcgaagg acttgtggtc gagcaggtgg
     4201 aggacgttcg cgcgccgccc cgtgaagaag ttgtcgaggc agagcacctc gtgcccctcg
     4261 tcgaggaggc gctcacacag gtgcgagccg atgaagcccg ccccgccagt tacaagtatt
     4321 ctcatcttct tttgtgacct tggctgactg atatccgaca tctgaaaagg gaacgcggat
     4381 taaacggata aggacggatc aaatccgtgt tcatccgtac tcatccgtgt tctctcacgc
     4441 cgtggccgcg ccttcactcg cgcgcgcacg cgcgccgtcg gcgccgacca cttcctcccc
     4501 cgcccctgcc ggagtctccc gccggccctg ctgttttcgc ttccgccgcc ggcgcgtctg
     4561 ctcgacgcgg ccgcccgccg tggcgagggc ggcgagggtg aggaagagga ggccgttaga
     4621 ggtcgtgtgc agggggaagt cgaagaagct gtggacgagg gcggcgaaac aaccggaaag
     4681 cgcgccgagg gcgacgccgc ggcggaaggc gtcggttgac tgcatgcgcc ggagcgccat
     4741 cgcgaagagg ccggcgacga agaggagtcc gagcgcgaag gcgacgatgc cgccgtcgga
     4801 gagcagttgg aggtagtcgt tgtgcgcctg ttccagacga tagatgctgc cgttgctcgt
     4861 gtcgtagcgc gggtagacgg cgctgaaagc gcccaggccc gcgccgagcc acgggtgggc
     4921 gcggatgatc tcgaccgtgc cgcgccagaa gtggaggcgc ccggtggtcg ggtcctcgga
     4981 gttgaccgtg ccgagcaggc ggttgaggct gtcctcgccg ccgaagaaga cgaccccggc
     5041 gatgagggcc acgacgatgc cgaggcccgc ggccgcgcgc agcagcagtg cgcgcgcgcg
     5101 cgtgtgctcc tgagcgtcct cgccgccgtc gcccccatgg tgcgccggcc gccgcaccag
     5161 tcccgagacg acgacgagga agaagacctc ggcgactaag gccagcatcc cgccgcgcga
     5221 gttcgtcatc atgagcgcga tgcccatcag cacggcgctg aagagataaa gcaggcggcg
     5281 ctcgcgctcg atggcgccgg cgaagagcag acccagcggc agccccgcgg ccatcaccat
     5341 gtagttggcg aagtggtggc ggttgatgaa ggggccgaag ccgagggcct gccgggtctc
     5401 gcgcaggccg aagatgcggt cggggctgac gaagaactgg atgaggccga agagggcgag
     5461 cgcgaagccg aagacgatga cggtgcgcac gatgagccgg agacggcgag cgctgtcgat
     5521 gaaggcgagc gcggcggcga agtagacgaa cagccccgcg agttggatga gcacgaggcg
     5581 cgtggcgtag gggtcgaagg agagcgtgcg cgcgggcgcg gtggcgagcg ccgcggcggg
     5641 gtcgcccggc gcgggcgcgc ccgcgagcgg gaggagttgg aagaggccga ggaggaagag
     5701 gcccgcgagt ggaagttgga ggaagttctt gctgaagcgc aggcggcggg cgcgccaggc
     5761 gtcggccgcc cagagcgcga cgacgacgcc ggccgtcccc tgaaagacgg cgagcgacca
     5821 gttatgcacc gtgccgaagg cgagcgtcga gaggacgagc gcgagacaga gcgcgaggac
     5881 gatgtaacgg ctgaaggcgg tgcgctccgt gttgggtgcg cccttcggcc gcgtcgtcag
     5941 cgcccgctca ctgtcctttc ggcgccggcg cgccggccgc ggcgcttccg ccgacacgtt
     6001 ggagattgaa gtcgtcatac cagactttac cgaagatggg gcacgcgccg tccgagcacc
     6061 ccacgcgcac gagcctgagc gtcacggcct cggccgccgg cgccgtaaac tccacgatca
     6121 cctgctgcca gtcgctcgtc cccgccggcg cggccgcgcc gcccgcgaac gccaccccgg
     6181 cggcgtccgt gacctgaacg acgagcgtcg ccgcgctctt cacctcctcc gtgcggacgg
     6241 cgtaggagag gcgatagcgc gcccgcggct cgacgaggac gagctgcgag aactgaccca
     6301 acgtgaactc gctcggcgcg ctgaactcca gacgcaggga gcgccgtccc tcgcgggcgt
     6361 tgcgcgcgtc aatgttcatt tgcgcctgcg ggacggggcg gacgatccag tcgaaagggt
     6421 tgccggagtt ggccgtcacg tcgcgctcga agccgccgtt gaagagtttt cccacggcga
     6481 catcctgcgc ggccgccggc tgcacctcgc cgtagacctc cagcgccttg cggtaacgtc
     6541 cggcttcgat gagcgcgttc atcagactcg cgcccgaggc ggcctgctct ctgcgctcct
     6601 cggtcttgag cgtcccccac agaatcagcg cctcgtccag acgcttctgc gtgagaagat
     6661 gttggacgag acgggcgcgc gcctcgtagg agtcgccgac ggccgcgccc acggcgcgca
     6721 cgtcgccgcc gaagtagctc cacgcggtgg tgaagatttg ggtgtgcagc gtggggtcga
     6781 tctcggcggc gcggcgcagc tcggcgaacg cctccggctc gcggcccgcg cgcagcagca
     6841 ggttgccgag ataccagcgc ggcatcgagt agtgcggggc gagcgagacg gcgtggcgca
     6901 gggcgagcgc gccgccctcc tcgtcgccgg cctgtccacg cgcgcggccc agttccatcc
     6961 acagacggtg gtcgtgcggc gcaagcgaca cggcgcgctc gtagcggacg agcacctcac
     7021 ggagttcctc gggcgcgaag ctcttgcggg cgaagacgcc gagcgtgtag tgaacctgcg
     7081 ggtcgtcggg cgcccacctc tcggcggcgt gcaggatgcc gccgatttgc gccagcgcct
     7141 cctgcacctg cgggtcgttg gggcggacgc gcgccgcggc gatcaccgat tcgaccgccg
     7201 gcgggttcga cgccagcgtg cttcccatac accagcgcgc cgccgtccac gacgcgaaca
     7261 gcaccgccaa cgccggcagc accaccaaca cgcgccgcca cagcgcctcg acgccgacgg
     7321 tcttgatctg atccttatcg ggcataatta agggagtcag aagtcaggag tcagaagtcg
     7381 gaatgagagg cggcttcatc ttcttcattc cgacttctga ctcctgactt ctgactcctt
     7441 gcactcttca ttccccgctc aggtcgtcga tctggatacg catgcgtttg acggagacgc
     7501 gcgcgtccga gcaggtgcgg acgatatcgc gcacgcgatc ctcggagagt ttgaggctgg
     7561 agatgagcac ctcctcgacg tgcagcgtct cgcagatgcc gcgcagcttg ccgttgccgc
     7621 cgaagacccg gaggccgtgg atgaccttcc ccatcttgtg ccggtcgtcg tcggcgaaac
     7681 cgacgggggc gtaattgagt tcggggttgt tgagtatctc gcgcaggagc agttcgccgg
     7741 cgtcgcccgc gccgtagatg agcacgcgcc gccccgtgcg cgacgacccc atcggcatca
     7801 ccttgcggag catgcggaag gcgacgcggc tcccggcaag cagcacgaag aggatgatgg
     7861 cgtcgaggac gaagacggag cgcgagtagt cctgaaagcg gaaggcgaag aggagcacca
     7921 gcacgctcgc caccgacccg gcaacgaccg ccttgacgta gaccaccaga ttgtcgaggc
     7981 tcacgtagcg ccagatgccg cggtagacgc ccgccacgag gaaggccccc atcttgacga
     8041 agacgagcac cggcaccgtc cgcaggaaca gctccgtcgc cccgatctcg ttgacggggc
     8101 cgtagagcag caggtacgag gcgtagaagg agaggatgat gagcgccgcg tctaagaaga
     8161 cctcgaagac tctgcgcttg taggagagat cgacgaggaa ggcgacgagc ggcttgtcgc
     8221 gcgccgcgcg cacctcctcc tcgtcgtaga ccttgacgcc ggcgagatag acgccgacga
     8281 gcgtcagcac caccgtgaac ccgagcacgg ccgcgaggct cacgtcgaac gcgacctcgc
     8341 gcacgaagag ggccagcagc cctgagagcg ccgcgaatcc gtagagcatc cagacggcct
     8401 tgcgctccga catccccaga gcgaccagac ggtgcgacgt gtggtcgcgc ccgccctgtg
     8461 agaccccgcg cccggagagc ttgcggatga gggtgacgaa ggtggtgtcg aagatcggga
     8521 tgaagagggt gagcacgggg acggccagca ccggcaggaa cgagcgcgag cgcccgcccg
     8581 aggcggcgag gagcgaggtc gaggccagga agaagccgat gaacatcgag ccgcagtcgc
     8641 ccatgaagat ggtggccggg ttcgagttgt aaacgagaaa tccggcgagc gcggcggcga
     8701 agacggcgag catcagcgcc tcgatggcct gcccgttgcc gatgaagttg acggcgagga
     8761 aggccgaggc gatggcggcg atgccggcgg cgaggccgtc catgttgtcg agcaggttga
     8821 cggcgttcgt aatcccgatg agccagaaga tcgtgatcgc catgttgacg ggcaccgacg
     8881 ccgtccacgg caaggtgagt ccgccaaggc cgaccgtcgc cgcgccgaag tagatgagca
     8941 tggcggcgcc catcatctgc ccgatgagtt tctggtacgg cttgatgtgc aggaggtcgt
     9001 cgatgagtcc gacgaggaag aggaaggaac tggcggccat cacggcgagt ccctgcggcg
     9061 tgtgcgggac gaaggccgcg tagatgataa tcaccgtcag gaagacggcc aagcccccga
     9121 gcatcgccgt cggcgcccga tgccagcggt cgggcttcgg ctgcgcgacc atcttgcggc
     9181 tgcgcgcgta ggcgcgcacg agcggggtga gcacgagcgc cagcgcgagc gccgccgcga
     9241 cggccagaag ttgttcggtg gtgaagagca tgaatccggg gactgaatga cgacgcctct
     9301 gtcgggacgc ttgaaaagct attcgatgcg gcgtgagaaa tcaagcgggc aggcttgaga
     9361 aaacagtacc gcgagcggta gcgagcgggt acgcctgtga tgggaggccc gccgcgcgca
     9421 cgcgacaccc gcttgctacc gctcgcggta ctgtcccccg ttacgggcgg cgcgctgacc
     9481 cggcgcgatg cgccgggcta tcacacggcc gccccttcgg gacttcccaa acagattctt
     9541 aagcttgacc gccggccgcc gcgggccgcc ggccgtagta gtcgatgacg gtgcggagga
     9601 tttcgtcgag cgtgacggtg gggcggaagc cgacggcccg ctcgatcttc gaggtgtcgg
     9661 ggacgcggcg cggcatgtcc tcgaagccct cctcgtaggc ttcatcatag ggcacgaaga
     9721 cgatctctga cttcgacccc acgagcgact tcacgcgctc ggccagctcc agaatcgaga
     9781 cctcctcgtt cgagccgatg ttgaagacct gaccgaccgc ctcctcggtc tccatcagct
     9841 tgacgagcgc gcccaccacg tcgccgacgt agccgaagca gcgcgtctgt ttgccgtcgc
     9901 cgtagatggt gatcggcgtc ccggcgagcg cctgcttgac gaaggtcggg atgaccatgc
     9961 cgtactgacc cgtttggcgc ggcccgaccg tgttgaagag gcggacgacg accgtgggca
    10021 gccgttgctc gcgccagtag gcgagggcga ggaattcgtc gatggccttc gagcaggcat
    10081 aagaccagcg ccccttcgtc gtcgcgccca tcacgaggtt gccgtcttcg cgaaacggca
    10141 cctccgacga cagcccgtag acctccgagg tcgaggccac cagaaccttc ttcttcttct
    10201 tcgccgcctg cgcgagcacc acttcggtgc agtggacgtt cgtttcgatg gttcgcaccg
    10261 gcgactcgac gatgagcttg acgccgacgg ccgccgcgag gtggaagatc acgtcgcact
    10321 gatcgacgag ttcggccatg agccggcggt tattgacgct gtcgatggtg tagtggtagc
    10381 gcgggttcgc cttcaggtgc gcgatgttgt cgaagctccc cgtggagagg tcgtcgatgc
    10441 agaagacctc gtcgccgcgc gccatgtagg catccgtgag gtgtgagccg atgaagccgg
    10501 cgccgcccgt gattagtact cgcaaatgtc ttctccctct tttaagacgc gcggccccgg
    10561 cgggcgcgcg tgatgtccgt gcccggcccc gcggcgcgcg caccttgctg cgccgcgctc
    10621 tcggccgggt cgattaactc ttcgtagagc ctgtggatgt ccgccagcag gcgttcgacc
    10681 gaatactggc gcgcgacgaa ctcaaggccg cgcgcgccca tcccgagccg cagctcctcg
    10741 ttctcgatca ggtagtcgag cgcgcggctg aaactctcgg cgtcgcactc ttcgaccaac
    10801 acgccgcgct cgcacacctg ccagccgcgc ggccggcgct gtagcagcgc ggggtcgaga
    10861 ccgccgagga ggtcgggcac gccgcccacg gccgtcgcga tgacgggccg cgcgttggcc
    10921 atcgcctcga ccagcgtcag cggcgtcccc tcgttcagag aggtgagcgc caccacgtcg
    10981 agcgccgggt agaagttctc cgggtcgtcg cgcgtgccga ggaatatgac atcgtcaatg
    11041 atgccgagcg aggccgcctg cgcttcgagc tgccggcgca gcagcccgtc gccgacgacg
    11101 acgaagcgca cgcgccgaag cgtccgccct tcggtctccg cctcgcgcgc ggcgtggcgc
    11161 ttgtagtgct cggccgccgc gaggaagagc gcgtggttct tgatcgcggt gaggcggccg
    11221 acgatgccga ccaatatctc gtcgtcgccc gcgccgagtt cgcgccgcag ccgcgggcgc
    11281 cgctcggccc agtcggcgaa ggcgcgcatg tctaatccga gcgggatgac ggcgaactgc
    11341 gcggggcgcc cgacgcggaa gtgtccgccg atctcttcga gctggcgggg gctgatgacc
    11401 acgacgcggt cggtggcgac gcgcgccagc gcccgctcga tggcgaggaa gagacgcgtc
    11461 ttccgagggc cgtagtagct gtggaagacg tgcccgtgat acgtgtgtac gaagcgcacg
    11521 cttcggggac ggccccacag ggccgagggc gtgagccagc ggtagagcag cccggccacg
    11581 cgtcccacgg tgccggcctt cgccgtgtgc gtgtgtacca cgtcggggcg ctcgcgcacg
    11641 agcagtcggt agagcttcca gacggtgacg gcgtccttcg gcgagacctc gcggctcatc
    11701 tccgggatga tgtggggccg cacgcccaag gcttcggaga agtagcccat gtcgtcttcg
    11761 cccggcggca cggtgcccgc caccagcacc gtctccacgc cgcgctccac cagccccgcc
    11821 gacagccacg ccacgtggcg cgccggcccg cccacgttca gtctggcaat gatgcggacg
    11881 actttcataa gggagctgtt agctaatagc tgttagctgt tagctaaaac tgtcagtggt
    11941 cgcttgtctg caccgggcag ctcaacgctc gcgtcgaagg gcggaaggct cgacgaacaa
    12001 acaactgact acggactacg gacaaagaac agcctttagc taacagctaa tagctaatag
    12061 ctaaccgcta atcgctttca cgtcttccat cttgttagcg cccgtctcgg cgacgaggtt
    12121 cgaggcgtgg aggaggctct cgaaggtgcc ggcgtccgtc caccagcctt cgagttcgtc
    12181 ccacgacatc tcgtcgcgcc cgatgtaggc gttgttcacg tcggtgatct ccagctcgcc
    12241 gcggcccgag ggcttgaggg tcttgatgat gcggaagacc tcggcgtcgt acatgtagat
    12301 gccgatgacg gcgtagtcgg actttggctc ggcgggcttc tcctcgatgc ggacgacgcg
    12361 ccggccgtcg agttcgggca ctccgaagcg ttgcgggtcg gacaccttct tgagaaggat
    12421 tttggcgccg ccgccctgat gacggtagga gcgcacggcc cgcccgatgc tgccctcgat
    12481 gatgttgtcg ccgaggacga cgcagatggg ttcgccgtcg gagaagtctt cgcacaggtc
    12541 gagcgccgcg gcgatgccgc cctcgccctc ctgataggtg tagttgaggt gcttgaggcc
    12601 gaagtccttc ccgtttttga ggagcttgag gaagtcgccg gccgagttgc cgcccgtgac
    12661 gatcatgatg tcacgcacgc ccgcgttgac gagcgtctga atcgggtaat agatcatcgg
    12721 cttgtcgtag acgggcagca gatgcttgtt cgtcaccgac gtgagcggcc tcagacgcgt
    12781 ccccagccct ccggctaaca cgacaccttt cattaagcgc ctccgatgtt gagctaatag
    12841 ctgttagctg ttagctgaag aaaaccaacc ggaaattttg actcgtggcg gccacgtccg
    12901 cgacggcccc tgccgtgagc tgacagttag tgtccgcccc cttgttcagt cgatgaatct
    12961 ctggaaccag agttccagca tcaggagtat ccagagatgg tgggagcggt caagcccgcg
    13021 cacggtgtgc tcttcgacga ggccgcgcac gcgctccggt ttgaagaagc cgcggcccag
    13081 cgatttttcc gagagcagcg tctcgcgcag gaacggctcc atgcgcccgc ggaaccagtg
    13141 gccgatgggc acgccgaagc ccatcttgcc gcgcgtcaga ttctcctccg gcaccagccc
    13201 ccgcagcgcg cgcttcaaaa gatacttcgt ggtgcgcccg cgcagcttca ggtgctccgg
    13261 gagcgaggca gcgaactcga tgacgtggtg gtcgaggaat ggcgagcgcg cttccaagct
    13321 tacggccatg ctcgcgatgt ccactttgac gagcaggtcg ttgggcaggt aggtcatcgt
    13381 gtcggcgagg agcgccgcgt ccaccacgcc cgccccgttg gccatcgtga accacggcgc
    13441 gatcaagtcc gacgccctct gcatgcgcgt ctcggcccga aatccttcgg tgtagagcat
    13501 ctgcttcgcc gcgtcgtcga agacgctcac ccactgcgcg taacgctcgg ccgaggggag
    13561 cgaggcggcg cgcacgaacc gcttcacccg gccgaggtag ctgcgccgca cctcggagga
    13621 cggcatgagc gagagcgccg gctcgatgaa cgcgccgcgc agggccgcgg gcacgcggcg
    13681 ataggtctca gcaagacgca tcgccgcgta acggtcgtag ccggcgaagc actcgtcgcc
    13741 gccgtcgccg tttaaggcca cggtgacgtg ccggcgcgtc tcgcgcgcga catagtaagt
    13801 cggaatggcc gaggagtcgg cgtaaggctc gccgtagtgc tcgacgaggg tcggcagcac
    13861 ttccatggcg tcggggcgga cgatgaactc gtggtggtcg gcgccgacga gttcggcgac
    13921 gcggcgcgcg tggtgaagct cggagaagtc ctgctcgtcg aagccgatgg agaaggtctt
    13981 gacgggcgcg ctcgactcct cgctcatcag cgccacgacc gcgctcgaat cgatgccgcc
    14041 tgagaggaac gcgccgaggg gcacctccga catcagacgc acgcgcacgg cgtcgcgcaa
    14101 aagctcgacg gcgcgctcgc tcgcctcctc ttccgtccac ttctccttcc ggttgaagtc
    14161 gggcatccag taacgctcaa tcttaatctc gccgtcgcgc gtgagacgca aagagtggcc
    14221 cggctccagc ttgcggatca tgcggtaggc cgtgagcggg gccggcacgc acatgaagga
    14281 gagatagcgg tcgattgcct cgtggtcgat gtcgcggctc acctcggggt gctgaagcag
    14341 cgcctgaaac tcggagccga agacgaagtc gcccgagggc gtccgggcgt agagcagggg
    14401 ctttttgccg acgcggtcgc gcgcgaggaa tagctcctgc ttgcgttcgt cccagatggc
    14461 gaaggcgaac atgccgcgca ggtgtttcgg cgcgtcggag ccgtagcgcg tgtaggcgtg
    14521 gacgaccgct tcggtgtccg agtcggtgta gaactcgtgg ccgaggtcgg cgagttgctt
    14581 gcgaagctcc ttgtagttgt agatttcgcc gttgaagacg atccacgccg agcgatccgc
    14641 gttgtggatg ggctgctggc cgccggcgag gtcgatgatc gagaggcggc gcatggcgag
    14701 gccggtgcgc tccccgtggt agaagccgtc ctcgtcgggg ccgcggtggc ggatggcctc
    14761 acacatcgag gccagcagcg cctcgtcaac ctgtcgcccc tcgcggcgca cgatccctgt
    14821 gatgccacac attccctaaa aacctatttt ctccgacaac cggtcggcaa tcgactgcca
    14881 ctcctgcgcc tgcttgcgcc gggccaaggc cagcgccgtc gcattctgcg ccaacagcct
    14941 cgcctggcgg gcctcgcgct gcttatcctc cttcgtctcc ctggcgatga cgaggccgac
    15001 ggaggcgaag atgaagtaga agacgagcag ttgcgagcgc tgccgatagg ccgtccccac
    15061 gttgccctga aagaccgaat acgccagcgt cagcatcgtc gtgaatacca atatcggcaa
    15121 cgcctgccgc atccggtagc gcacggtgaa ccacagcccg acgacgagga aggggaaagc
    15181 cccccaccac accagcatct cgggcagcgt caggctctgc cggaggttga ccaactgcca
    15241 cgggaacggc gcgaacaaca ggtagatcat ccctacgggg atcgccgtga tcgc
//
LOCUS       DASVES010000345        23098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_216411, whole genome shotgun sequence.
ACCESSION   DASVES010000345 DASVES010000000
VERSION     DASVES010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23098
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216411"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1360
                     /locus_tag="VF240_14080"
     CDS             26..1360
                     /locus_tag="VF240_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7176388.1"
                     /translation="MKFIRYSKFHGFDVSGVNLGDLMEGLQDSLLDSGFDDDYYWTRN
                     RRDPDTSLDALRQALLQALMEMGEISRRDVEQMLADNDGQFRGSQLEELLNQLIERLV
                     EEGYLKLREETLAERMRRQGQAGPGEVGEPLPKNVKFEVTEKGLDFLGYKTLRNLLGS
                     LGKSSVGRHDTPELATGVEAAAGPKPYEFGDTVNLDVNTTLLSAIRREGLGVPLNLEY
                     SDLHVQQCDYQSSCATVVMLDCSHSMILYGEDRFTPAKKVALALAHLIRTQYPGDSLK
                     VVLFHDDAEELPLARLASTQVGPHHTNTAEGLKLARRLLQAQRKEMKQIVMVTDGKPT
                     ACFLDDAPATAATARGGARRFGEIHKGRRLYKNSMGGDPVVMEATFREVQACRKAGVQ
                     VNTFMLATDSYLVEFVKQMTAMTNGKAYFATPGNLSQFVLIDFLKRKKSRVK"
     gene            1440..1949
                     /locus_tag="VF240_14085"
     CDS             1440..1949
                     /locus_tag="VF240_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7176389.1"
                     /translation="MDTIWKTIIWQQFGASIDMLENALLACPDEMWGERSEQPEYWYL
                     IYHTLFWLDLYLSGSVEGFSPPAPYTLDELDPAGVMPERVYAKEELQTYLRHCRRKCR
                     ATIESLTDEKAHQLCVFGRGEVSFAELLLYNMRHVQHHSAQLNLILRRKTNSAPGWVA
                     RAKNSHGRE"
     gene            2216..3544
                     /locus_tag="VF240_14090"
     CDS             2216..3544
                     /locus_tag="VF240_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176390.1"
                     /translation="MRAKSIYSGVALLVSVLSCPNFAAAQGAPIDASVARQYFGEART
                     ACEQDGGKLWGRSLCGPMLFVDPLTRAVVANQKDAEGLLTAKDDVFVGRLSDRQPVAN
                     AYITWAGVRWTMIMWPFMTKDPLQRVKLMMHESFHRIQNDLKFPAVGGANDHLDTRDG
                     RVWLQLEWRALRQALTSGGAARRRAVEDALTFRKYRRSLFPKAEAAERELEMNEGLAE
                     YTGFKLSAAAEPQLIAYLTRQIEQAPGRPTFVGSFAYSSGPAYGILLDASLPDWRKGL
                     TPQDDLGELLRRAHRIKLPSDLKARAEKQAAQYDAAALRLTESAREATRRKLIAEYRM
                     RLVDGPTLLAPKTATGRVAFNSTNIVPLEGVGTVYPTATVRDEWGVLEVSNGALMVSE
                     GGRITKVYVPAPADLDARPLKGDGWTLELNSGWVVIPGERKGDYILKRPE"
     gene            complement(3578..5548)
                     /locus_tag="VF240_14095"
     CDS             complement(3578..5548)
                     /locus_tag="VF240_14095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176391.1"
                     /translation="MARRVLSIALRYAIALVAYALVILLPIGLQRLFKISFDVTPLVI
                     AVMIASAWYLGTGPGLVVAFAFELTLDYFAAKAGADPFTAKAAAIAFNRLVLFVSLVV
                     FAGSRRGAERRLREQREWLRVTLSSIGDAVIATDLDGSVNFINPTAEALTGWTTAEAI
                     RKPLGQVFRVISEETREPVESPFSIIKREGLVVGLANHTTLIARDGREIPIEDSGAPI
                     KDSDGRVIGVIVVFHDVSERRRAAREREQMLKREQAARLAAESADRIKDEFLATVSHE
                     LRTPLTAILGWAAMINLGPLEEETVRNALDVIERNARSQAQIIDDILDVSRIINGKLR
                     IDSRPVVFASVVQAAIDTLRPAAAAKDITLTTALEQTNVLVTGDPDRLQQIIWNLVAN
                     SIKFTPKGGRVEVHLERTAAHLEVRVSDSGVGIDEQFLPHVFERFRQADSSTTRAHGG
                     LGLGLAIVRHLAELHGGTVLAESDGTGRGASFTVRLPQANGRDESAATHAGESRVGPA
                     RTEPALMGGTGDSSADEPDLTGLRVLVVEDEPDTLEILRVALGQYGAQVRAASSSAGA
                     LEAFSEWKPDVLVSDLGMPGEDGFTLIRKVRSLAPEQGGSVPAAALTAYVREEDRLRA
                     LAAGYQVHVPKPVEPSALASVVARLARRAGGS"
     gene            complement(5692..6351)
                     /locus_tag="VF240_14100"
     CDS             complement(5692..6351)
                     /locus_tag="VF240_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7176392.1"
                     /translation="MSHTPAHEELKEQAALYALGALETAEARDFERHLAEGCEACAAE
                     LRDFEGVAAQFALASAPARPPAGAREKLLSRIAAAAEEPRPEVSPEGAPAPDVLALAP
                     DLLVVRRDAGEWEETPDPGVFVKMLFVDREHDTVTRLVRLDPGARIPSHRHLGYEQCL
                     IVEGGMRAGHLHLRAGDFNCAAPGTIHEEISTEEGALLLIVAPANYEVLGGKREHGFN
                     G"
     gene            6758..8800
                     /locus_tag="VF240_14105"
     CDS             6758..8800
                     /locus_tag="VF240_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX7176393.1"
                     /translation="MNYPRPSLRLSLCLATLVAVFCAPPAALALGDDWRPIEASELAL
                     KSPVVEPDADAEVLFWDVRIDDDQADLVFKHYVRIKVFTDRGRESQSKVDIPYLTKFV
                     QIKDVAGRTIKADGSILELKKSDIFERDIVKANGLKLKAKSFAMPGIEAGAIIEYRWR
                     EVRPGAMVRYQRLHFQRDIPMQRVTYHVKPLQSPYFPFAMRYQQFRTQAGSKFEKEKE
                     GFFAFTQTNVPAFREEPHMPPEDQVRSWMLVYYSPDTNLEPAKYWKDYGRRVHELFKG
                     RVKGGDDVKKAAAEIVGDAATPEQKLERIYEFCRTKIQNVSDDASGMSAAEIEKAKEN
                     KSASDTLKRGVGNGGDIDMLFGALASAVGFDVRVAMAADRGDIFFDATFADDYFINPA
                     SIAVRVGEGWRFFNPGYGYLPFGMLRWQEEGTATLVTDPKEPTFVLPPVSAPEKSLQK
                     RIARLKLSEDGTLEGDVRLEYTGHYAVEMKEYNDDDSPAEREETLKGRIKSRLPGAEI
                     SNIRVENVTDYAKPFAYQFHVRVPGYAQRTGKRLFIRPAFFQFGLDPLFPTSARRHAV
                     YFHYPWSEDDSVEIGLPEGFALDNAEAPTPYGADAISTYKPTLAVTKDGRTLVYKRQF
                     TFNSRTAEGSPMILPAGGYSRVKAFFDEIHKQDSHTVSIKQGAAATPTATSDAAKP"
     gene            8971..10953
                     /locus_tag="VF240_14110"
     CDS             8971..10953
                     /locus_tag="VF240_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HEX7176394.1"
                     /translation="MLRRFITLSALLIVCAPAAALASTTAGDEPPAWLRQAAATAAPV
                     YDKKVPAVVLHDEGAMTVAPDGRVTTVSSYAVRVLTREGRGYASAIAYYETGTDKVKE
                     IRAWMLRADGTLVKKYGKDETVDAAVSLDDVYNEARARRISGVDEAEVGYVFGFQITT
                     EERPYFAQAKWYFQQRIPVLVSRMTLSLPAGWRASGVTLNHATVEPATVGNSYAWELR
                     NLQPIEPEPESPSVMTLAPRLAVNYAPAGAAVSSGAARAFSTWSDVSKWYSELSDPQA
                     EPDDELALKARELAAGAKTELEKIRAVGRFVQNIQYISIQIGIGGYRPHRATEVFAKK
                     YGDCKDKATLMRAMLRALGLKSYPVLIFSGDSTYVREEWASPTQFNHCIVAVRVSDET
                     QAASVVTHPVLGRLLIFDATDDHTPVGDLPDHEQGSWALVAAGAEGTLMRMPVTPPEA
                     NRLEREAEVTLTADGLITAVLRERSQGQSAVRQRGMFHQLSRADYQKMVEGWITTNGA
                     TLARLSKVEPTDDHAGGRFSLDVDFSAERYAQTMANRLLVFKPVIVSHTGMPAVGASQ
                     RKHPVVLKGRAFSETVRFKLPDGFEVDELPEALKLDSDFGSYAASYEVKDGQLLFKRT
                     LVQRASTVPADKYKLLRDFFGRIRAVEDSPVVLARK"
     gene            complement(11045..11905)
                     /locus_tag="VF240_14115"
     CDS             complement(11045..11905)
                     /locus_tag="VF240_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX7176395.1"
                     /translation="MHPKVILNGKLTDATRARLPLASSAHLHGRGVFTTVAIYAGRPL
                     LWPLHWARLREGAERLGVELAPHDEETVRADLLRLIEANGVGRGRARVTLLARAAHGL
                     WKLAGSDALASDLLLMTGEARAAENEVVALTVSPYRVNTLSPLVGVKSVNYLEPVLAW
                     EEARARDFDEAVRVNERGEVVSAAMANIFWVVEGRIHTPALSVGALAGTTRDCCIDIA
                     RDLSVPLVEGVYALHDLGEADEIFLTSAGLGVSLVTSFDFHTYTVSAGSVAARMREAF
                     RQVTLDKSKE"
     gene            11990..12175
                     /locus_tag="VF240_14120"
     CDS             11990..12175
                     /locus_tag="VF240_14120"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7176396.1"
                     /translation="MFTRRVRIPAEVSVSALDLRARGEFTIDRGDFNVRATSAFHGMV
                     RVRDKLKLTFDIVARQI"
     gene            complement(12490..14880)
                     /gene="purL"
                     /locus_tag="VF240_14125"
     CDS             complement(12490..14880)
                     /gene="purL"
                     /locus_tag="VF240_14125"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX7176397.1"
                     /translation="MIPPEVVSAHNLTPEEFSRIEELLGREPTMTELGVFSVMWSEHC
                     SYKSSRTHLGRLPTTGERVIVPPGENAGVVDVGDGWCAAFKVESHNHPSFIEPFQGAA
                     TGVGGILRDIFTMGARPVASMNSIRFGPLEHPEHGRRNRTLLAGVVAGIAHYGNAFGV
                     ATVGGEVAFDDAYSLNPLVNAFALGLVRRDQIFFGKAEGVGNPVLYVGAKTGRDGIHG
                     ATMASAEFDEEALEKRPTVQVGDPFLEKLLLEACLEVMRGGAVAGIQDMGAAGLTSSS
                     CEMAARAGNGLDIDLDLVPQREQGMTAYEIMLSESQERMLIVAHRGRERQVVDVFRKW
                     DLDAVVIGRVTDTGSVRVSQHGEMLADIPVKYLTDEAPRYERPMRAPQPGATTDDTVR
                     DEAKQVITEPAGTGCGGVPVNASVVNADEVQEILEAPSTVVSNSEADHDDALRRLLAA
                     PNLASKEWVYRQYDHMVRTNTAVLPGADAAVVRIKETRRALAMCLDGNGRYCAVSPRE
                     GAALVVAEAARNVVCVGARPIAITNCLNFASPERPEVMWAFSEVIDGMAEACRALDTP
                     VISGNVSFYNETEGRGIHPTPVIGMVGLIEDVRRVVQTGFKAEGHLIALLGETGDDLG
                     VSEYAATVEGRTVGEMIAAGARLPRLDLVRERAVQEAALRAIDEGLLQSAHDTSDGGI
                     SVAIAECCFSSLNRVGVGADVELTGDVPTTAQLFGETPSRIVVSFEESARARVEEIAA
                     AANCPISVIGRTGGARLRVAVNGRACVSQDLGELERAWRGTLADKLQAEVLAAAAE"
     gene            complement(14993..15691)
                     /locus_tag="VF240_14130"
     CDS             complement(14993..15691)
                     /locus_tag="VF240_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7176398.1"
                     /translation="MTKTRFAKVICTSAALLLVAAGAPAYTAARGQQGTTLARSKSAG
                     ARKQKKRGAQATARKSNTGAAATKKGLPGVVAASASLPRVREIDEAGLKALLEEHAKG
                     GRRLLVNFWATWCTPCREEFPDLVKIDQEFAGSEDFEFITISTDEVAEIGTSVPTFLA
                     EMNAAAIPAYLLNAKDPEAAIALIDKSWHGDLPATFLFGPGGEILFAHKGRVKADELR
                     AAIKDSGKTVISDK"
     gene            complement(15702..16307)
                     /locus_tag="VF240_14135"
     CDS             complement(15702..16307)
                     /locus_tag="VF240_14135"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7176399.1"
                     /translation="MKRFSACALLLVASLALCVSAFAGGEDTPAAGAAIGSTVADFKL
                     PDAEGKERTLASLAGAKGTVLIFVSTQCPVSNAYNERMEKLAQEMKARGVSVVGINSN
                     VAEKRDDIKSHAAERGLSFAILKDEGNRIADRLGAGVTPEAFLLDANNRLVYRGRIDN
                     SRSGDAVTSSELRDAIDAVLAGKPVAKSEVRAFGCSIKRAS"
     gene            complement(16541..16714)
                     /locus_tag="VF240_14140"
     CDS             complement(16541..16714)
                     /locus_tag="VF240_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176400.1"
                     /translation="MPKWLLYGLAAAGQFAAAAALFFYGDRVVIPVVLIFAGLCMTAA
                     AVGSAIEARRGKA"
     gene            complement(16757..17278)
                     /locus_tag="VF240_14145"
     CDS             complement(16757..17278)
                     /locus_tag="VF240_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176401.1"
                     /translation="MPIRDDILRLAEWWADPGEYKPWPEELISFFEVAEAEQAPSYDE
                     AQKSLTTLSTGAYVGGAVRHWCGIFACHVLREAGANVRWTLLGGKIVGKGANQVKYVP
                     GNQGMQPGDVAMIPKSNHHFIVTGVDYGSNTLWSVDGNTSGQMIRSLTKPIKYSSQEA
                     AAKTIYGYYRVLV"
     gene            complement(17334..17489)
                     /locus_tag="VF240_14150"
     CDS             complement(17334..17489)
                     /locus_tag="VF240_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX7176402.1"
                     /translation="MSEQRLTCADCGREFAFSAEEQKFFNEKGFTPPKRCKECRQAKR
                     EQRGERR"
     gene            complement(17486..17830)
                     /locus_tag="VF240_14155"
     CDS             complement(17486..17830)
                     /locus_tag="VF240_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176403.1"
                     /translation="MTEKETLQSAADEARKTARAAREFAALLDDYAKHVTQPGWWQKA
                     DDLHYSAMSKLRVVNAGLAACNKFIAASGNGAPAPPAERAGEKKAGAKREAAAEAPKT
                     PTVRFVRKGGKK"
     gene            complement(17831..18127)
                     /locus_tag="VF240_14160"
     CDS             complement(17831..18127)
                     /locus_tag="VF240_14160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEX7176404.1"
                     /translation="MKLISTDEAAERLGVSGRRVRQLIDEGKLAAQYVGGGYVIDETA
                     LEGVKVYGKPGRPPSDNGQKKASREALKRKALARWEGEGGKLAPAKKSAKKKGK"
     gene            18724..19860
                     /gene="hisZ"
                     /locus_tag="VF240_14165"
     CDS             18724..19860
                     /gene="hisZ"
                     /locus_tag="VF240_14165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX7176405.1"
                     /translation="MSESLSKVPGGMRYYFGAEARLRRHVEDTAMSVFDGWSYEEITT
                     PSVDYYALFEFGMGHEEARRAFRFTDTDGRMLALRPDVTSSVARAAATLLARRERPLR
                     LCYTGQVFRQQSPSHAHWRRESEQLGCEHIGSRQGAADVEILAVAAELLGRLGLDGRF
                     SITLNDVGVFNGVAERLALDPEARDRMRRLIDIRDAAELRRFLAPFTREEEARAFARL
                     VELPGKSELLTEARRLIKNTRSAEALERLEYLWHVVEALGLSDKFEVDLGDLSGLDYY
                     TGLTFKIYVEGVGTRAGGGGRYDQLTANFGRPEPAVGFVLDLDAITGALSQTAALAPA
                     SEPERRAERLAGDDAGALFLRALERRAGGGRVILDAGEAERCRS"
     gene            19848..20486
                     /gene="hisG"
                     /locus_tag="VF240_14170"
     CDS             19848..20486
                     /gene="hisG"
                     /locus_tag="VF240_14170"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7176406.1"
                     /translation="MQELTIAIPKGRLQAPTLERLGRVGIDAPDEALTSRRLWVKDTR
                     ERYRFIFVKPVDVPVYVEHGIADCGVVGRDVLLESEADLLQPLDLKIGRCRVVVASHK
                     PELLSNGFGMMRVATKYPRIAAEYFGSRGIPVEIIELAGSVELAPVLGLSDCIVDLVE
                     TGRTLDENGLRVVALVAECTARLVVNRASFQLKAGAVSQLIRALGESAEAER"
     gene            20487..21818
                     /gene="hisD"
                     /locus_tag="VF240_14175"
     CDS             20487..21818
                     /gene="hisD"
                     /locus_tag="VF240_14175"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7176407.1"
                     /translation="MRERVKGLIEIVAREDVEARARVLRRIMGRSIALDADVLERVAK
                     IVADVRRRGDAALVDYARQFDGVALNPAELRVDEESLRRTAARVEADVLAALRESIRS
                     VRAFHEFERQDSWEVEAGRGVRLGQRVTPVGRAGLYVPGGTASYPSSVVMNVVPAQVA
                     GVGRIVVATPPRTLHENPSVAAALVELGVTEVYAVGGAQSVAALAFGTETVPRVDVIT
                     GPGNKYVAAAKKLVFGAVGIDSIAGPSEVVIVADETARADFVAADLLAQAEHGDDASA
                     VLITTSAALAEGTSFELVRQARDLPRRSIVERSLAQYGAIIIVGNVKEACELVNELAP
                     EHLEVIAREEERIVKGVRSAGAIFCGAHAPEAVGDYLAGPNHVLPTGGAARFSSALGV
                     HNFIKRTSIIRYTPAELERTAPMIAALAGAEGLEAHARSAMIRLKGGGQDA"
     gene            21811..22881
                     /gene="hisC"
                     /locus_tag="VF240_14180"
     CDS             21811..22881
                     /gene="hisC"
                     /locus_tag="VF240_14180"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7176408.1"
                     /translation="MRSPSESVKARVRELRPYALSPERARVKLNQNENPWDAADAIKR
                     ETLERVRERAWSRYPDFVPAALHERLAAFTGWRPDGVIAGNGSNELIQALMMVTVGEG
                     ARVLISEPTFALYRQIATILGGEVRSLPLTPEFEFDADALLTDVRVSQPALTIICSPN
                     NPTGSVMANDDLAALAEASRGLLVIDEAYFEFAEETAAPLLEQYPNIVILRTFSKALA
                     MAALRVGYLLAAPELAREVAKAVLPYNLNALSQTAAEVAVESYETQFQPLIKRIIRER
                     ERLYAEMSRVPGLAPVRSRGNFLIVRSAIEPRRIFDELLARDILVRDVSGYPMLKDYF
                     RVSTGTPAENDLLIAALREIFT"
     gene            22878..>23098
                     /locus_tag="VF240_14185"
     CDS             22878..>23098
                     /locus_tag="VF240_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176409.1"
                     /translation="MTSNSLTTETRTDAPQQTTARSATVRRQTKETDVSVALTLDGGG
                     NARVNTGVAFLDHMLELFARHALFDLAVE"
BASE COUNT         4000 a         7674 c         7469 g         3955 t
ORIGIN      
        1 aagtaccaga gccaatgggg aagtaatgaa gtttatccgt tattccaaat tccacggctt
       61 cgacgtgtcg ggcgtcaacc tcggcgacct gatggagggc ttgcaggact cgctgctcga
      121 ctccgggttc gacgacgact actactggac gcgcaaccgg cgcgaccccg acacgtcgct
      181 cgacgcgctg cggcaagctc ttttgcaggc gctcatggag atgggcgaaa tctcccggcg
      241 cgacgtggag cagatgctcg ccgacaacga cgggcagttt cgcggctcgc agctcgaaga
      301 gttgctcaac cagttgatcg agcgcctcgt cgaagagggc tatctgaagc tgcgcgagga
      361 gacgctcgcg gagcggatgc ggcggcaggg tcaggcgggg ccgggcgagg tgggcgagcc
      421 tctgcctaag aacgtgaagt ttgaagtgac cgagaagggg ttagattttc tcggctacaa
      481 gacgctccga aaccttctgg ggagcctcgg caagtcgtcc gtcggccgac acgacacgcc
      541 ggagctggcg acgggcgtgg aggccgccgc cgggccgaag ccctacgagt tcggcgacac
      601 cgtcaacctc gacgtgaaca ccacgctcct gtcagccatc cggcgcgagg gtctgggtgt
      661 gccgctcaac ctcgaatact cagacctcca cgtccaacaa tgcgactacc aatcgtcgtg
      721 cgccaccgtc gtcatgctcg actgctcgca ctcgatgatt ctctacggcg aggatcgctt
      781 cacgcccgcc aagaaggtcg cgctcgcctt ggcgcatctc atccgcacgc agtaccccgg
      841 cgactcgctc aaggtcgtcc tcttccacga cgacgccgag gagctgccgc tcgcgcgtct
      901 ggcctccaca caggtcggcc cgcatcacac gaacacggcc gagggcctca agctcgcgcg
      961 ccgtctcctc caggcgcaac gcaaggagat gaagcagatc gtcatggtca ccgacggcaa
     1021 gcccaccgcc tgcttcctcg acgacgcgcc ggcgacggcg gcgactgcgc gcggcggcgc
     1081 ccgccgcttc ggcgagatac acaaggggcg gcggctctac aagaactcga tgggcggcga
     1141 ccccgtcgtg atggaggcca ccttccgcga ggtgcaggcg tgccgcaagg ccggcgtgca
     1201 ggtcaacacc ttcatgctcg ccaccgacag ctacctcgtc gagttcgtca aacagatgac
     1261 ggccatgacg aacggcaagg cctacttcgc cacgcccggc aacctctccc aattcgtgct
     1321 catcgacttc ctcaagcgca agaagagccg cgtcaagtag tcgccgcatc ggcgccgcct
     1381 gaggggtcct cggtgaaacc gcaaacgccc tccccccgct ctcgctgggc tttccttcaa
     1441 tggataccat ttggaagacg atcatctggc agcaattcgg ggccagtatc gacatgctcg
     1501 aaaatgccct gctcgcctgt cccgatgaga tgtggggcga gcgctccgag cagccggagt
     1561 actggtatct gatttatcac acgctcttct ggctcgacct ttatctgtcc ggctcggtcg
     1621 aaggtttttc gccgcctgcg ccttacacgc ttgacgagct ggacccggcg ggggtgatgc
     1681 ccgaacgcgt gtatgcgaaa gaagaactcc aaacttacct gcggcattgt cgccggaagt
     1741 gccgggcgac gattgaatcg ctaacggacg agaaggcgca tcaactctgt gtgttcggcc
     1801 ggggcgaggt cagcttcgcg gaattgcttt tgtataatat gcgtcacgtt cagcatcact
     1861 ccgcacagct caacctgatt cttcggcgga agaccaattc cgcgccgggc tgggttgcca
     1921 gagcgaagaa cagccacggc agggagtgaa ggttagcgta gacctgtgga aaggaagccc
     1981 gttgacgaat atttcaaagc atcacaacag ggcaattaca ttacagcgcg acctcttcga
     2041 gattcctgac ggggtgacgt ttctgaattg cgcgaatcgc gccgcatctt tacaacgatg
     2101 aatccgacat cgaaaggctt ttcagcgtgc taagagaatt tgcttgaaag gcttgtgccg
     2161 ctcatcgcga cgccgaacaa gtcattagaa tcgacagata ctttcgggag gaaccatgcg
     2221 agccaaatct atttactccg gcgtcgcact actggttagt gtcctgtcgt gcccgaactt
     2281 cgccgcggca caaggcgcgc cgattgacgc gtccgtcgct cgtcagtatt tcggcgaagc
     2341 gcggacggcc tgtgaacagg acggtggcaa gctgtggggt cggtcgcttt gcggccccat
     2401 gctgtttgtc gatccgttga cgcgcgcggt ggtcgcaaat caaaaggacg cggagggcct
     2461 tctcaccgcg aaggatgacg tgttcgtcgg gcgtctgtcc gacagacagc ccgtggcgaa
     2521 tgcttacatc acgtgggccg gcgtgcggtg gacgatgatc atgtggcctt ttatgaccaa
     2581 agaccctctt caacgagtta agttgatgat gcacgagtcg tttcatcgca tccagaacga
     2641 ccttaaattt ccggcggtgg gcggcgcgaa cgaccacctc gatacgaggg acgggcgtgt
     2701 ctggctgcaa ctcgaatgga gggcgctgcg gcaagctctg acgagtgggg gggcggcgcg
     2761 gcggcgcgcg gtcgaagacg cactcacgtt ccgcaagtac cgtcgctcac tgttcccgaa
     2821 ggcggaggcg gccgagcgcg aactggaaat gaatgaaggc ctcgcggaat acacggggtt
     2881 caagctgagc gccgcggccg agccgcaact aatcgcctat ctcaccagac agatcgaaca
     2941 ggcccccggc aggccgactt tcgtcgggtc tttcgcttac agttccggcc ccgcctacgg
     3001 gattctgctg gacgcttccc tcccggactg gcgcaaaggt ttgacgccgc aggacgatct
     3061 gggagaactg ttgcggaggg cgcaccgaat taagctcccg tccgacctga aggcgagggc
     3121 tgaaaaacag gcggcccagt acgacgccgc cgcgctcagg ctgacggagt ccgcgcgcga
     3181 agcgacgcga cgaaaactga ttgccgagta tcgtatgcgc ctcgttgacg ggcctacctt
     3241 gctggcgccg aagaccgcca cggggcgcgt cgcttttaac tcgaccaaca tcgtcccgct
     3301 cgaaggcgtg gggacggttt atccgaccgc gacggtgcgc gacgagtggg gcgtgctgga
     3361 ggtctcgaac ggggcgctga tggtcagcga gggtggcagg attacgaagg tgtatgtccc
     3421 tgctccggcc gacctcgacg cgcggcccct gaagggcgac ggctggacgc tggagttgaa
     3481 ttccggctgg gtagtgattc cgggcgagcg caagggcgat tacattttga agcggcccga
     3541 atagtaatca gagttccatt cagaggcggg cgagcgttca agacccgccc gcgcgccgcg
     3601 ccagcctcgc caccaccgac gccagcgccg acggctcgac cggcttgggg acgtgaacct
     3661 gatagccggc ggcgagcgcg cggaggcgat cctcttccct gacgtaggcg gtcagcgcgg
     3721 cggccgggac ggagccgccc tgctccggcg cgagggagcg cactttacgg atgagggtga
     3781 agccgtcttc gcccggcatc cccaggtcgg agaccaacac gtcgggcttc cactccgaga
     3841 aggcttcgag cgcgccggcg ctcgacgagg cggcgcgcac ctgtgcgccg tactggccga
     3901 gcgccacgcg gagaatctcc agcgtgtccg gctcgtcctc gaccacgagc acgcgcaggc
     3961 ccgtcaggtc cggctcgtcc gccgaactat cgcccgtccc ccccatcagg gccggctccg
     4021 tccgagccgg cccaaccctc gactcgcccg cgtgtgtcgc agccgactcg tcgcgcccgt
     4081 tcgcctgcgg cagacgcacg gtgaacgacg cgccgcgccc cgtgccgtcg ctctccgcca
     4141 gcaccgtccc gccgtgtagc tcggcgaggt gccggacaat cgccagaccc agaccgaggc
     4201 cgccgtgcgc gcgcgtcgtc gaagagtcgg cctgtcggaa tcgctcgaag acgtgcggca
     4261 gaaactgttc gtcgatgccg acgccgctgt cgctcaccct cacttcgagg tgcgccgccg
     4321 ttcgctccag atgaacctcg acccgcccgc ccttcggcgt gaacttgatg ctgttcgcga
     4381 ccagattcca gatgatctgt tgcaggcggt cggggtcgcc ggtgaccaga acgttggtct
     4441 gttcgagcgc ggtcgtgagg gtgatgtctt tggcggcggc ggccgggcgc agggtgtcga
     4501 tggcggcctg gacgaccgag gcgaacacga cggggcgcga gtcgatgcgc agcttgccgt
     4561 tgatgatgcg cgagacgtcg aggatgtcgt cgatgatctg cgcctgcgac ctcgcgttgc
     4621 gctcgatcac atcgagcgcg ttgcggacgg tctcctcttc gagcgggccg aggttgatca
     4681 tggccgccca gccgaggatg gccgtcaggg gcgtgcgcag ctcgtgcgag accgtggcca
     4741 gaaactcgtc cttgatgcgg tcggcggact cggccgcgag gcgcgccgcc tgttcgcgct
     4801 tgagcatctg ctcgcgctcg cgcgccgcgc gccggcgctc gctcacgtcg tggaagacga
     4861 cgatcacgcc gatgactcgg ccgtccgagt ctttgatggg ggcgccgctg tcttcaatcg
     4921 ggatttcccg gccgtctctg gcaatcagcg tggtgtggtt ggccagcccg acgacgagtc
     4981 cctcacgttt gatgatcgag aacggactct cgaccggctc gcgcgtctcc tcgctgatga
     5041 cgcggaacac ctggccgagc ggcttgcgga tggcctcggc ggtcgtccag ccggtgaggg
     5101 cttcggccgt cgggttgatg aaattgacgg agccgtcgag gtcggtggcg atgacggcgt
     5161 cgccgatgct ggagagcgtc acccgcagcc actcgcgctg ctcgcgcaga cgccgctcag
     5221 cgccccggcg cgagccggcg aacaccacca gactcacgaa gaggacgagg cggttgaagg
     5281 cgatggccgc ggccttcgcg gtgaaggggt cggccccggc cttggcggcg aagtagtcga
     5341 gtgttaattc aaaggcgaag gcgacgacca gccccggccc cgtccccaga taccatgcgc
     5401 tggcgatcat caccgcgatg accagcgggg tcacgtcgaa acttatcttg aacaggcgct
     5461 ggaggccgat ggggagcagg atgaccagag cataggccac cagcgcgatg gcgtagcgca
     5521 gcgcgattga tagaactctt ctcgccatct tgaattctct gaaccgagat tgcggcgtcg
     5581 agtttatggc ctcgcgaggg tgggttgcat tcgtccgtga aatggtgcgg ggggaacacg
     5641 gataagacgg attggacgga tcgacacgga tttgatccgt gcttatccgc ctcatccgtt
     5701 aaatccgtgt tccctcttcc cgcccaacac ctcgtagttc gcgggcgcga cgatcaggag
     5761 cagcgcgcct tcctccgtgg aaatctcttc gtggatagtg cccggcgcgg cgcagttgaa
     5821 gtcgccggcg cgcaagtgta ggtggcctgc gcgcatgccg ccttcgacga tgagacactg
     5881 ctcgtagccg agatgacgat gcgagggaat gcgcgcgccg gggtcgaggc ggacgagtct
     5941 ggtcacggtg tcgtgctccc tgtccacgaa gagcatcttg acgaagacgc cggggtcagg
     6001 ggtctcctcc cactcgcccg cgtcgcggcg gacgacgagg aggtctggcg cgagcgcgag
     6061 cacgtccggc gcgggcgcgc cctcgggcga cacttccggc cgcggttcct cggcggcggc
     6121 agcgatgcgc gacaggagtt tttcgcgcgc gccggccggc ggacgggcgg gggcggaggc
     6181 gagcgcgaac tgtgcggcca cgccctcgaa gtcgcgcagc tcggcggcgc acgcctcgca
     6241 gccttcggcc aggtggcgct cgaagtcgcg ggcctccgcc gtctccagcg cgccgagcgc
     6301 gtagagggcc gcctgctcct tcaactcttc gtgtgcgggc gtgtgactca tttcgagcgg
     6361 cttcggtcct ctgtcgtcat gtcggtgagg acgcgcggca gaaggccgta gacgaggcgg
     6421 ctcatctcgt cgcgggcgta ctataacatc aaattcgggc gccggccgaa atgtcccggc
     6481 ggtcgggcgc gcgcggcgcg ggacgcacgc cgggagggag aaagcggccg ccggccgccc
     6541 ccgccctcaa ctcccggcgc gcccggttca gactttgaga tttgcttcca ctttcgtggt
     6601 attttccgct tgcgcttttt cgcttcgcct gtataaaatc tcatgcgtcg ctacctctcg
     6661 cggggcaaac ccggcgcccg accccgcctc cgacaatctt ctccacgtca aagccacgca
     6721 cgaacgcgcg cggcctcccc gatgaaaggt cgatagtatg aactaccccc gtccctcgct
     6781 ccgcctttcg ctgtgtctgg cgacgctcgt cgccgtcttc tgcgcgccgc ccgcggccct
     6841 cgccctcggc gacgactgga ggcccattga agcgtccgaa ctcgcgctca agtcgccggt
     6901 cgtcgagcct gacgccgacg ccgaagtcct cttctgggac gtgcgtatcg acgacgatca
     6961 ggccgacctc gtcttcaagc actacgttcg catcaaggtc ttcaccgacc gcggacgcga
     7021 gtcgcagagc aaggtggaca tcccctacct caccaagttc gtccagatca aggacgtggc
     7081 cgggcgcacc atcaaggccg acggctccat cctcgaactc aagaagagcg acatcttcga
     7141 gcgcgacatc gtcaaggcca acgggctgaa gctgaaggcc aaatcgtttg cgatgccggg
     7201 catcgaggcc ggcgcaatca tcgagtaccg ctggcgcgag gtgcggcccg gcgcgatggt
     7261 cagataccag cgcctccact tccagcgcga catccccatg cagcgagtca cctatcacgt
     7321 caagccgctc caaagcccct acttcccctt cgccatgcgt taccagcagt tccgcacgca
     7381 ggcgggcagc aagtttgaga aggagaagga aggcttcttc gccttcacgc agacgaacgt
     7441 cccggccttc cgcgaggagc cgcacatgcc gcccgaggat caggtgcgct cgtggatgct
     7501 cgtctactac tcgcccgaca ccaatctcga accggcgaaa tattggaagg actacggcag
     7561 gagagttcac gagttgttca aggggcgggt aaaggggggc gacgatgtga agaaggcggc
     7621 cgccgagatc gtcggcgacg ccgcgacacc cgagcagaag ctcgaacgca tatacgagtt
     7681 ctgccgcact aaaatccaga acgtgagcga cgacgcctcg gggatgagcg ccgccgagat
     7741 cgagaaggcc aaggagaaca agtcggcgtc agacaccttg aagcgcggcg tcggcaacgg
     7801 cggcgacatc gacatgctct tcggcgccct ggcctcggcc gtcggcttcg acgtgcgcgt
     7861 ggcgatggcg gccgaccgcg gcgacatctt cttcgacgcg accttcgccg acgactactt
     7921 catcaacccg gccagcatcg ccgtgcgcgt cggcgagggg tggcgcttct tcaatcccgg
     7981 ctacggctac ctgccctttg ggatgctgcg ctggcaggag gaagggacgg ccacgctcgt
     8041 caccgatccg aaggagccga ccttcgtcct cccgccggtc tcggcgcccg agaagagcct
     8101 ccagaagcgc atcgcccgtc tgaaattatc cgaggacggc acgctcgaag gcgacgtgcg
     8161 tctcgaatac acgggccact acgccgtcga gatgaaggag tacaacgacg acgactcgcc
     8221 ggccgagcgc gaggagacgc tcaagggccg catcaagtcg cggctgccgg gcgcggaaat
     8281 ttctaacatc cgcgtcgaga acgtcacgga ctatgcaaag ccgttcgcct atcagtttca
     8341 cgtccgagtg cccggctacg ctcagcgcac gggcaagcgt ctcttcatcc gcccggcctt
     8401 cttccagttc ggcctcgacc cgctcttccc cacgagcgcg cgccgtcacg cggtctactt
     8461 ccactacccg tggtcggagg acgactccgt cgagatcggg ctgcccgaag ggttcgcctt
     8521 agacaacgcc gaggcgccca cgccctacgg cgccgacgcc atcagcacct acaagccgac
     8581 gctcgccgtg acgaaggacg ggcgcacgct cgtctacaag cgccagttca ccttcaacag
     8641 ccggacggcc gaaggctcgc cgatgatcct gcccgccggc ggctactcgc gcgtcaaagc
     8701 cttcttcgac gagattcaca agcaggactc gcacaccgtc tcgatcaagc agggcgcggc
     8761 cgccacgccc acggccacgt ctgacgccgc caagccgtag aaggtttgag aagtgataag
     8821 tgatgagtga tgagtgataa gaaggagcta ttagcgatta gctgttagct gtttggctga
     8881 gagctaatcg ccaacagcta acagctctta tcactcatca cttatcacgt agtcctggcc
     8941 ttattgcctc gcacacgaaa gatgaaagaa atgcttcgac gatttatcac actctcggcg
     9001 ctgctcatcg tctgcgcccc ggcggccgcg ctcgcctcca cgacggccgg cgacgagcct
     9061 cccgcgtggc tgcggcaggc agccgccacg gccgcgcccg tctacgacaa gaaggttccg
     9121 gccgtcgtcc tccatgacga gggggcgatg acggtcgccc cggacggacg cgtgaccacg
     9181 gtgtcgagct acgcggtgcg cgtcctcacg cgcgaggggc gtggctacgc ctcggccatc
     9241 gcctactacg agacgggcac ggacaaggtc aaagagattc gcgcgtggat gctgcgcgcg
     9301 gacggcacac tcgtcaagaa gtacggcaag gacgagacgg tggacgccgc cgtgagcctc
     9361 gacgacgttt acaacgaggc gcgcgcgagg cgcatctccg gcgttgacga ggccgaggtc
     9421 ggctacgtct tcggcttcca gatcacgacc gaggagcgcc cctacttcgc gcaggcgaag
     9481 tggtactttc agcagcgcat ccccgtcctc gtctcccgca tgaccttgag cctgccggcg
     9541 ggctggcgcg cctcgggcgt cacgctcaac cacgcgacgg tcgagccggc cacggtcggc
     9601 aactcctacg cgtgggagct tcgcaacctc cagcccatcg agccggagcc ggaaagcccc
     9661 tcggtgatga cgctcgcccc gcgcctcgcc gtcaactacg cgcccgccgg cgccgccgtg
     9721 tcgtcgggcg cggcccgcgc cttctcgacg tggtcggacg tgtcgaagtg gtatagcgaa
     9781 ttgtctgacc cacaggccga gccggacgac gagctggcgc tcaaggcgcg ggagctggcg
     9841 gccggcgcga agaccgagct tgagaagatc agggccgtcg gccgcttcgt ccagaacatc
     9901 cagtacatct ccattcagat cggcatcggc ggctaccgcc cgcaccgcgc gacggaagtc
     9961 ttcgccaaaa agtatggcga ctgtaaggac aaggcgacgc tgatgcgcgc gatgctgcgc
    10021 gccctcggcc tcaaatccta ccccgtgctc atcttctccg gcgacagcac ctatgtccgc
    10081 gaggagtggg cctcgcccac gcagttcaac cactgcatcg tcgccgtccg cgtgtcggat
    10141 gagacgcagg cggcgagcgt cgttacgcac cccgtgctcg gtcgcctgct catcttcgac
    10201 gcgaccgacg accacacgcc cgtgggcgat ctgcccgacc atgaacaggg gagctgggcg
    10261 ctcgtcgccg ccggggccga aggcacgctc atgcggatgc ccgtgacgcc gcccgaggcc
    10321 aaccgactgg agcgcgaggc cgaggtcacg ctgacggccg acggcctcat cacggccgtc
    10381 ttgcgcgaac gctcgcaggg gcagtcggcc gtgcgccagc gcgggatgtt ccaccaactg
    10441 tcgcgcgccg actatcagaa gatggtcgag ggctggatta cgaccaacgg cgcgaccctc
    10501 gcccggctct cgaaggtcga gccgacggac gaccacgccg gcgggcgctt ctctctggac
    10561 gtggacttct ccgccgaacg ctacgcgcag acgatggcga accgcctgct cgtcttcaaa
    10621 cccgtcatcg tctcgcacac ggggatgccg gcggtcggcg cgtcgcagcg caagcacccg
    10681 gtcgtcctca agggccgggc cttcagcgag accgtccgct tcaaactccc cgacggcttc
    10741 gaggtggacg aactgcccga agccctcaag ctcgactccg acttcggctc ctacgccgcc
    10801 tcctacgagg tcaaagacgg ccaactcctc ttcaagcgca cgctcgtcca acgcgcctcg
    10861 accgtccccg ccgacaagta caaactcctc cgcgacttct tcggccgcat ccgcgccgtc
    10921 gaagactcgc ccgtcgtcct tgccagaaaa taacagtacc gcgagcggaa gcctcgcggg
    10981 tctagcgtgc gttgaggcaa gttgccatca cagccggacc cgctcgctac cgctcgcggt
    11041 actgctactc tttcgacttg tctaaagtca cctgccggaa cgcctcgcgc atgcgggcgg
    11101 cgacgctgcc ggccgagacg gtgtaggtgt ggaagtcgaa ggaggtgacg aggctcacgc
    11161 cgaggccggc ggaggtgagg aagatttcgt cggcttcgcc gaggtcgtgc agggcgtaga
    11221 cgccctcgac gagcggcacg gagaggtcgc gcgcgatgtc gatacagcag tcgcgcgtcg
    11281 tgccggccag agcgccgacg gagagcgcgg gcgtgtggat acgcccttcc accacccaga
    11341 agatgttggc catcgccgcc gagacgacct cgccacgctc gttcacgcgc acggcctcgt
    11401 cgaagtcgcg cgcgcgcgcc tcctcccacg cgagcaccgg ctccagatag ttgacgctct
    11461 tgacgccgac gagcggcgag agcgtgttca cgcggtaggg cgagaccgtg agcgcgacga
    11521 cttcattctc cgccgcgcgg gcctcgcccg tcatcaacag caggtcggag gcgagcgcgt
    11581 cggagccggc cagcttccac aacccgtgcg ccgcccgcgc cagaagtgtg acgcgcgcgc
    11641 ggccccgccc gacgccgttg gcctcgatga gcctcaggag gtcggcgcgc acagtctctt
    11701 cgtcatgcgg cgcgagttcc accccgagcc gctcggcgcc ctcgcgcaga cgcgcccagt
    11761 gcaacggcca gaggagcggc cgccccgcgt agatggcgac ggtcgtgaac accccgcgcc
    11821 cgtgcaggtg cgccgaactc gccagtggca ggcgcgcgcg cgtcgcgtcc gtgagcttgc
    11881 cgttgaggat gaccttcggg tgcatctttg gaagccccgc acgtcggttt aactttccga
    11941 aagtctacga acacgcggag gatacaacga acggcgcgcc ggaagctaac tgttcacgcg
    12001 gcgcgtccgc atccccgccg aggtttccgt gagcgcgctc gacctacgcg cgcgcggtga
    12061 gttcacgatt gaccgcggcg acttcaacgt ccgcgcaacc tccgccttcc acggcatggt
    12121 gcgcgtccgc gacaaattga agctcacctt cgacatcgtc gcgcgccaga tttgagcctc
    12181 gcagagcgcg aaggaaaaag ataacagggc cgcgagcgcc gtcaggcgcg gaagaatgta
    12241 gcccacggcg tgagccgtgg ggttagcgac acaacataga ccaagccccg gaggggcgaa
    12301 agaatggtga cgggcttcgt gctcaacgcc gccattcttt cgcccctccg gggcttttaa
    12361 atctgtcacg tcccgttacc cacggctcac gccgtgggct acattcttcc gccgcttgcg
    12421 cggctggttg acgcccgcgc tcaagcctcg cttatcaaac gtctgcacac gattcacgtc
    12481 acttcgatct cactccgccg ccgcggccaa cacctccgcc tgtaacttgt cggcgagtgt
    12541 gccgcgccac gcccgctcta attcgcccaa gtcctgcgag acgcacgcgc ggccgttcac
    12601 cgcgacgcgc agacgtgcgc cgcccgtccg cccgatgacg ctgatgggac aattcgccgc
    12661 cgccgcgatc tcctcgacgc gcgctcgcgc cgattcttcg aaactgacga cgatgcgcga
    12721 cggcgtctca ccgaagagtt gcgccgtggt gggaacgtcg cccgtgagtt ccacgtccgc
    12781 gcccacaccc acgcggttga gcgaggagaa gcagcactcg gcgatggcga cactgatgcc
    12841 gccatccgag gtgtcgtgcg ccgactggag cagcccctcg tcgatggcgc gcaaggccgc
    12901 ctcctgcacc gcgcgctcgc gcacgaggtc gagtcgggga aggcgcgcgc cagccgcgat
    12961 catctcgccc accgtgcggc cctcgaccgt cgccgcatac tcgctcacgc cgaggtcgtc
    13021 gcccgtttcg ccgaggagcg cgatcaaatg cccttcggcc ttgaagcctg tttggacgac
    13081 gcgccgcacg tcctcgatga gacccaccat gccgatgacg ggcgtcgggt ggatgccgcg
    13141 cccctcggtc tcgttataga aggagacgtt gccggagatg accggcgtgt cgagcgcgcg
    13201 gcacgcctcg gccatgccgt cgattacttc ggagaacgcc cacatcacct cggggcgctc
    13261 gggcgaggcg aagttgagac agttcgtgat agcgatgggg cgcgcgccga cgcagacgac
    13321 gttgcgcgcg gcctcggcga cgactaacgc cgcgccctcg cgcggactga cggcgcagta
    13381 gcgcccgttg ccgtcgagac acatggcgag cgcgcgtcgc gtctccttga tgcggacgac
    13441 ggccgcgtcc gcgcccggca gcacggccgt gttcgtcctg accatgtggt cgtactgcct
    13501 gtagacccac tccttcgagg cgaggttggg cgcggcgagc aggcggcgca gcgcgtcgtc
    13561 gtgatccgcc tctgagttcg agacgaccgt gctcggcgct tcgagaattt cctgcacctc
    13621 gtccgcattc acgaccgaag cgttgacggg gacgcccccg cagcccgttc cggcgggttc
    13681 agtgatgacc tgcttggcct cgtcgcgcac ggtgtcgtcc gtcgttgcgc ccggttgcgg
    13741 cgcgcgcatg gggcgctcgt agcgcggcgc ttcgtccgtc agatacttga cgggaatgtc
    13801 ggccagcatc tcgccgtgct ggctgacgcg gacgctaccc gtgtccgtga cgcgcccgat
    13861 gacgacggcg tccaagtccc acttgcggaa cacgtcaacg acctgtcgct cgcgcccgcg
    13921 atgggcgacg atgagcatgc gctcctgcga ctccgagagc atgatctcgt aggccgtcat
    13981 gccctgttcg cgttggggca cgaggtctaa gtcgatgtcg agaccgttgc cggcgcgcgc
    14041 cgccatctcg cacgacgaag aggtcagccc cgccgcgccc atgtcctgaa tgccggccac
    14101 cgccccgccg cgcatcactt cgaggcacgc ttcgagcagg agtttttcga ggaaggggtc
    14161 gcccacctgc accgtgggcc gcttctccag cgcctcctcg tcgaactcgg ccgaggccat
    14221 cgtcgcgccg tgaatgccgt ctcttccggt cttcgcgccg acgtagagga cgggattgcc
    14281 gacgccttcg gccttgccga agaaaatctg gtcgcgtcgg acgagaccga gggcgaaggc
    14341 gttgacgagc gggtttaagg agtaagcgtc gtcgaacgcg acctcgccgc cgacggtggc
    14401 gacgccgaag gcgtttccgt aatgcgcgat gccggcgacg actccggcga ggagcgtgcg
    14461 gttgcggcgt ccgtgctcgg ggtgttcgag ggggccgaag cgaatgctgt tcatcgaggc
    14521 gacggggcgc gcgcccatcg taaagatgtc gcgcaggatg ccgcccacgc ccgtggccgc
    14581 gccctgaaac ggttcgatga aggaaggatg gttgtgcgac tcgaccttga aggccgcgca
    14641 ccagccgtcg cccacgtcca ccacccctgc gttctcgccc ggcggcacga tgacgcgctc
    14701 gcccgtcgtg ggcagccggc ccagatgcgt gcgcgaagac ttgtacgaac agtgctccga
    14761 ccacatgacg gagaagacgc ccagctccgt catcgtcggc tcgcgcccga gcagttcctc
    14821 aatgcgggag aactcttccg gggtcaggtt gtgtgcggag actacttcgg gaggaatcat
    14881 aggaaagtga taagtgatga gtgataagtg ataagtgcaa tggtaaagag cgagaaggat
    14941 tctatcttgt cacctgtcgc ttgtcactca accactcatc tcttatcact catcacttat
    15001 cacttatcac cgtcttcccg ctgtctttga tggccgcgcg cagctcgtcg gccttgacgc
    15061 ggcccttgtg cgcgaagagg atttcgccgc cggggccgaa gaggaaggtg gccgggaggt
    15121 cgccgtgcca gctcttgtcg atgagggcga tggcggcctc cgggtctttg gcgttcagca
    15181 ggtaggcggg tatcgcggcg gcgttcatct cggcgagaaa cgtggggacg ctcgtgccga
    15241 tctcggcgac ctcatcggtc gagatggtga tgaactcgaa gtcttcggag ccggcgaact
    15301 cctgatcaat cttgacgagg tcggggaact cttcgcggca gggcgtacac cacgtcgccc
    15361 agaagttgac gagcaggcga cggccgccct tcgcgtgctc ttcgagcagg gcctttaagc
    15421 ccgcctcgtc gatctcgcgc acccgcggca acgacgccga cgccgcgacg acgccgggca
    15481 gccccttctt cgtcgccgcc gcgcccgtgt tcgacttccg cgccgtcgcc tgcgcgcccc
    15541 gcttcttctg cttccgcgcg ccggcggatt tcgagcgcgc gagcgttgta ccctgctggc
    15601 cgcgagcagc cgtgtacgcg ggtgcgccgg cggcgacgag caggagtgcg gcgctcgtgc
    15661 agattacttt tgcgaaccgg gttttggtca tgaattgatt ttcaggacgc gcgcttgatc
    15721 gagcagccga aggcgcgcac ctccgacttg gcgacgggct tgccggcgag cacggcgtca
    15781 atcgcgtcgc gcagctcact cgaagtgacc gcgtcgccgc tccgcgagtt gtcgatgcgc
    15841 ccgcgataga cgaggcggtt gttcgcgtcg agcaggaagg cttcgggcgt cacgccggcg
    15901 ccgagccggt cggcgatgcg gttgccctcg tccttgagga tcgcgaagga gaggccacgc
    15961 tcggcggcgt gcgacttgat gtcgtcgcgc ttctcggcga cgttcgagtt gatgccgacg
    16021 acgctgacgc cgcgcgcctt catctcctgc gcgagcttct ccatccgctc attgtaagcg
    16081 ttcgagacgg ggcactgcgt cgagacgaag atgagcacgg tgcccttcgc gcccgccagc
    16141 gacgcgagcg tgcgctcctt gccttcggcg tcgggcagct tgaagtcggc gaccgtcgag
    16201 ccgatggcgg cgccggccgc gggcgtgtct tcgccgccgg cgaaggccga gacgcagagg
    16261 gcgagcgagg cgacgaggag gagggcgcag gcggagaatc ttttcatgac gcggtagtcc
    16321 tttcgggaat ttcgttcaat cggggaagac gaaagattag cagcgcgggc gcggggccgg
    16381 caagcggggc gttgtaaagg ttgtgtaaaa aggggcgccg tgtaaacgcg ccgcgtcatc
    16441 aacgagtcgc ctgacatccg tcgtcatggt ttgcgcgaac gttgcgcgag cgccgtgccg
    16501 aaaagcagac tttccacgct gtccgaagcc cgctcgcgaa tcacgcctta cccctgcgag
    16561 cctctatggc gctccctact gcggcggccg tcatgcacag gcccgcgaag atgaggacga
    16621 ccgggatcac gaccctgtcg ccgtagaaga acaaggcggc ggccgccgcg aattggccgg
    16681 cggcggcgag gccgtacagt agccacttcg gcatcgtcaa aacccccttc tcgcggccga
    16741 gtccgcgcgg gcgtcttcag accagcaccc ggtaatagcc gtagatagtc ttcgcggcag
    16801 cctcctgcga cgaatactta atcggcttgg tcagggaacg aatcatctgc cccgacgtgt
    16861 tgccgtccac cgaccacaaa gtgttcgagc cgtagtctac cccggtgacg atgaagtggt
    16921 gattcgactt cgggatcatc gccacgtcgc cgggctgcat cccctgattg ccgggcacgt
    16981 acttgacctg attggcgcct ttgcccacga tcttcccgcc cagcagcgtc cagcgcacgt
    17041 tcgcccccgc ctcccgcagg acatggcagg cgaagatgcc gcaccagtgc ctgaccgcgc
    17101 ccccgacgta ggcgcccgtg gacagggtcg tcaggctctt ctgggcctcg tcgtaggaag
    17161 gggcctgctc ggcctccgcc acctcgaaga aggaaatcaa ctcctcgggc cagggcttgt
    17221 actcgcccgg gtcggcccac cactccgcaa ggcggagaat gtcgtcgcga atcggcatca
    17281 gtcactccct cctcacattg cttacgcggc tccgcttcgg gcctttcgca ctctcacctc
    17341 cgctcgccgc gctgctcacg cttggcctgc cgacactcct tgcaccgctt cggcggcgtg
    17401 aagcccttct cgttgaaaaa cttctgctcc tcggcgctga aggcgaactc ccgcccgcag
    17461 tcggcgcacg tcaatcgctg ctcgctcatt tcttaccgcc tttcctcacg aatcgcactg
    17521 tgggagtttt gggcgcttcc gccgccgcct cccgcttcgc cccggccttc ttttcgccag
    17581 cccgctcggc gggtggtgcg ggggccccgt tgccgctcgc cgcgatgaat ttattacaag
    17641 ccgcgaggcc ggcgttgacg accctcaact tgctcatcgc cgagtagtgc aagtcgtcag
    17701 ccttctgcca ccagcccggc tgcgtaacgt gcttcgcata gtcgtctaac agggcggcaa
    17761 attcccgcgc cgctcgcgcc gtctttcggg cttcatcggc cgcgctctgc aatgtctctt
    17821 tttccgtcac ttacttgcct ttcttcttcg ctgacttctt tgccggagca agtttgccgc
    17881 cctcgccttc ccaccgcgca agggctttcc gcttcaaggc ttcccggctg gccttctttt
    17941 ggccgttatc gctcggcggc ctgccgggct tgccgtacac ttttacgccc tccaacgcgg
    18001 tctcgtcgat cacgtagccc ccgccgacgt attgcgcggc gagcttgccc tcgtcaatca
    18061 attgacgcac gcgccgcccg ctcacaccca aacgctccgc ggcctcgtct gtacttatca
    18121 acttcatggc cgcggattat ggcactgaaa ataaacttcc gcaaccggaa acactctctt
    18181 accccaatct tccacccgag gtaaattttt cacgtcggaa agatgagaag cagggggcaa
    18241 ggcactgagc acgtcacgca acgggaatcc gcgaggctcg cctttaaaat tgaacgggga
    18301 cgacctgtaa aacttgtcgt cgcgtgtcga gttaaaactg tgcgcgtcgt ccttatcagt
    18361 gcggcccacg ccgaactcgc gtggagtcag cgcacgtttg acaatcggag agccggtcgg
    18421 ctataaaggc cactcgcaga caagagacga aatatagtta tgaattccca gagaatgcat
    18481 cacgtcaacg gcacgcacca ccatacccat cacgggcatg gcggcggcgc gttggcgact
    18541 gcgttcttcg gcgaagaaca tttgcgagac tagcgaaggc tcgcaaaaac agatcgcgac
    18601 aaacccgccg ccggtgactg aagaagtccg gcggcggatt ttgttttggg cgaagggcaa
    18661 aagggcgaac ccctatgacc gatgatgcga ccgatgcggc cgacagggcg gcgatggcga
    18721 tttatgtctg aatcactttc caaagttccg ggcggcatgc gctactactt cggcgcggag
    18781 gcgcgtctgc gccgtcacgt cgaagacacc gcgatgtctg tcttcgacgg atggtcttac
    18841 gaggaaatca ccacgccttc cgttgactac tacgccctgt tcgagttcgg tatggggcac
    18901 gaggaagccc ggcgggcctt caggtttacg gacaccgacg ggaggatgct cgcgctcagg
    18961 ccggacgtga cctcgtcggt cgcgcgcgcc gcggccacgc tcctcgcgcg ccgtgagcgc
    19021 cccttgcgcc tctgctacac cggacaggtg ttccggcagc agtcgccctc acacgcgcat
    19081 tggagacgcg agtcagagca acttggctgt gagcacatcg gctcccggca gggcgcggcc
    19141 gatgtcgaga tactcgccgt cgcggccgaa ctcctcgggc gtttgggact cgacggccgc
    19201 ttctccatca cgctcaacga tgtcggcgtg ttcaacggcg tcgccgaacg cctcgccctc
    19261 gaccccgaag cgcgcgaccg gatgcgccgc ctcatcgaca tccgcgacgc ggcggaactg
    19321 cgccgtttcc ttgccccctt cacgcgcgag gaagaggccc gcgccttcgc gcgactggtg
    19381 gaactccccg gcaagtcgga gttactgaca gaggcgcgga gattgataaa aaacacgcgc
    19441 tcggctgagg cgcttgagcg ccttgaatac ttgtggcacg tcgtcgaggc gcttggtctg
    19501 tcggataagt tcgaggtcga tctcggcgat ttgtcgggac tcgattatta cacgggcctc
    19561 acgttcaaaa tttacgtcga gggcgtcggc acgcgggccg ggggcggcgg gcgctacgac
    19621 caactgactg ccaatttcgg gcggccggaa ccggcggtcg gattcgtgct cgatctggac
    19681 gccatcaccg gcgccctctc gcaaacggcc gctctcgccc cggcgtcaga gccggagcgc
    19741 agggccgagc gattggcggg agacgacgcc ggcgcacttt tcctccgggc gcttgagcgt
    19801 cgcgcgggcg gcgggcgcgt catcctcgac gcgggggagg ctgagcgatg caggagttga
    19861 ccatcgccat ccctaaaggc cggctccagg ccccgaccct cgaacggctc gggcgtgtcg
    19921 gcatcgacgc gcctgacgag gcgctcacgt ctcgtcgtct gtgggtgaaa gacacgcgtg
    19981 aacgctatcg cttcatcttc gtcaaacccg ttgacgtgcc cgtctacgtc gagcacggca
    20041 tcgcggactg cggcgtggtc gggcgcgacg tgctcctcga atccgaagcc gacctcctgc
    20101 aaccgctcga tcttaaaatc gggcgctgcc gcgtcgtcgt cgcctctcac aagccggaac
    20161 tactctcgaa cggcttcggc atgatgagag tggcgacgaa gtacccgcgc atcgccgccg
    20221 agtatttcgg gtcgcgcggc atccccgtgg agatcatcga gctggccggg tcggtcgagc
    20281 tggcgcccgt gctcggcctg agcgactgca tcgttgacct tgtggagacc ggcaggacgc
    20341 tcgacgagaa cggcctccgc gtggtcgcgt tggtggccga gtgcacggcg cggctcgtcg
    20401 tcaatcgcgc aagctttcag ttgaaggccg gggcggtcag tcaactcatc agggcgctcg
    20461 gcgagagcgc ggaggccgaa aggtaagtgc gagaacgggt gaaaggtttg atcgagatag
    20521 tcgcccgcga agacgtggaa gcgcgcgctc gtgtgctccg gcgaatcatg ggccggagca
    20581 tcgcgctcga cgccgacgtg ttggagcgcg tggcgaagat cgtggcggac gtgcgccggc
    20641 ggggcgacgc ggcgctcgtt gactacgcac ggcagtttga cggcgtcgcg ctcaaccccg
    20701 ccgagctgcg ggttgacgag gagagcttgc gccgcacggc cgcgcgagtg gaggcggacg
    20761 ttctcgcggc gctgcgcgag agcatcagga gtgttcgcgc cttccacgag ttcgagcgac
    20821 aggactcgtg ggaagtggaa gcggggcgcg gcgtccgtct cggccagcgc gtcacgcccg
    20881 tcggccgcgc gggactgtac gtccccggag ggacggccag ctatccatct tccgtggtga
    20941 tgaacgtcgt gccggcgcag gtcgcggggg tcggccgcat cgtcgtcgcg accccgccgc
    21001 gcacccttca cgaaaacccg tcggtggcgg cggcgctcgt cgagctgggc gtcacggagg
    21061 tctacgctgt cggcggcgcg cagtccgtcg ccgcgcttgc cttcgggacg gagacggtgc
    21121 cgcgcgtgga tgtcatcacg gggccgggca acaagtacgt cgcggcggcg aagaagctcg
    21181 tcttcggggc ggtcggcatt gattctattg ccgggccgag cgaggtggtc atcgtcgccg
    21241 acgagacggc gcgcgctgac ttcgtcgccg cagacttgct cgcgcaggcg gagcacggcg
    21301 acgacgcctc ggccgtgctg atcacgacca gcgccgccct cgctgaagga acgtcctttg
    21361 agcttgtgcg acaggcgcga gacctgccgc gccgctcgat agtcgagcgg tctctcgcgc
    21421 agtacggggc gatcatcatt gtcgggaacg tgaaggaagc ctgcgagctg gtcaacgagc
    21481 tggccccaga gcaccttgag gttatcgcgc gcgaggaaga gcggatagtc aaaggggtgc
    21541 ggagcgccgg ggcgatcttc tgcggcgcgc acgcgccgga ggcggtaggc gattatctag
    21601 cggggccgaa tcacgtgctg ccgacgggcg gggccgcgcg gttctcgtcc gcgctgggcg
    21661 tccacaactt catcaagaga accagcatca tcaggtacac gcccgcggag cttgagcgaa
    21721 ccgcgccgat gatcgcggcg ctggcagggg ccgagggttt agaggcccac gcgcgctcgg
    21781 ccatgattcg tctgaaggga ggtgggcaag atgcgtagtc cgtcggagag cgtcaaggcg
    21841 cgcgtgcgcg agctgcgccc ctacgcgctc agccccgaga gggcgcgggt caagctcaat
    21901 cagaacgaga acccgtggga cgcagccgac gccatcaagc gcgagacgct cgaacgcgtg
    21961 cgcgagcgcg cgtggtcgcg ctaccccgac ttcgtgcccg ctgcactaca tgagcggctc
    22021 gccgccttca ccggctggcg gcccgacggc gtcatcgccg gcaacggatc gaacgagctg
    22081 attcaggctt taatgatggt gactgtcggc gaaggcgccc gcgtccttat ctcagagccg
    22141 accttcgcac tctaccgcca gatcgccacc atcctgggcg gcgaagtcag aagcctcccg
    22201 ctcacgcccg agtttgaatt tgacgcggac gctttactaa ccgacgttcg agtctcgcaa
    22261 cccgcgctca cgatcatctg ctcgccgaac aatccgaccg gctccgtgat ggcgaacgac
    22321 gatctggccg cgctggctga agcctcgcgg ggcctgctcg tcatcgacga agcctacttt
    22381 gaatttgcgg aggagacggc cgcgccgctc cttgagcagt atccgaacat cgtcattctt
    22441 cgcaccttct caaaggcgtt ggcgatggcc gcgctgcgcg tcggctatct gctggccgcg
    22501 ccggagctgg cgcgcgaagt ggcgaaggcc gtcctccctt acaacctcaa cgccctctcg
    22561 cagactgccg ccgaggtcgc cgtcgaaagc tacgagacgc agttccaacc gctgatcaag
    22621 agaatcatcc gcgagcgcga aagactctac gcggagatgt cgcgcgtgcc gggtcttgcg
    22681 cccgtccgct cgcgcggcaa ctttttaatc gtgcgttccg caatcgagcc gcgccgcatc
    22741 ttcgacgagc ttctggcgcg cgacatcctc gtccgtgacg tgagcggcta cccgatgctc
    22801 aaagattatt tccgcgtgag cacggggacg cccgccgaga acgaccttct catcgcagct
    22861 ctccgggaga ttttcacatg acgagtaact cactcaccac cgagacgcgg acggacgcgc
    22921 cgcagcaaac aacggcgcgc tccgccaccg tccgccggca gacgaaggag acggacgtca
    22981 gcgtcgcgct cacgctcgac ggcggcggca acgcgcgggt caacaccggc gtcgcctttc
    23041 tcgaccacat gctcgaactc ttcgcccgtc acgccctctt cgacctcgct gttgagtg
//
LOCUS       DASVES010000346         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_795859, whole genome shotgun sequence.
ACCESSION   DASVES010000346 DASVES010000000
VERSION     DASVES010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795859"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1479)
                     /locus_tag="VF240_14190"
     CDS             complement(46..1479)
                     /locus_tag="VF240_14190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7176410.1"
                     /translation="MGIIFERPLRRASALCLALLLAAAPAASQQSPPPQPTPATAGDA
                     TAASPAPAAANRAVSSADAAAQVTEFEVGGLKVLVKRRANSQTVVAGLFLRGGAQNVT
                     AANAGVESLMLDVATEASRNFPRERLRKELARMGTGLGNGVNYDYSALTLNTTRFNFD
                     RSWEIFTDVALRPSFVPEDFERVRNRVAVSMRNEADTPDSYLQVLQARAAYAGHPYLN
                     DPRGNSETISRLTLADVRRYHERAMQTSRLLLILVGDVDPQMIRERVAATFGKLPRGD
                     YRPVALPQLSFAAGSVEVTERALPTNYVQGVFSAPPLTSEDIYPMRIASSILQSRVVV
                     EVRYKRNLSYAPDSFLYSQGANVGGIYVTSTDANQSVRLMLEEIARLQREPISADELQ
                     GTVQHFLTRHYLAQETNAAQAGELAQYELLGGGWRNAFSFLDRLRAVTPADVQRVAKN
                     YIRNIRFVVIGDPKSVDKTIFTPQSGG"
     gene            complement(1567..3063)
                     /locus_tag="VF240_14195"
     CDS             complement(1567..3063)
                     /locus_tag="VF240_14195"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7176411.1"
                     /translation="MKRQLFVFALALTMAAGTFAAPVAAQRRQPAARPAPNNPAANPA
                     AQQQQQRAPVFQQAQYVNRVLPNGLEIIVLEDHSVPLVTVELAVKNGSYTEPPELNGL
                     SHLYEHMIFKANRAARDGAEYLKTIDQLGIIYNGQTQEELVNYYFTTTTPNFPVAMRF
                     LRDAALYPLFDPNQFERERAVVIGELDRHESNPFGYLQLAMNDKLFYKYPSRKNPGGS
                     RETVSKATTDQMRLIQGRYYVPNNSAIVVTGDVKPDDAYRLAEELFGGWPRRARNPFE
                     EFPPVEHPPLERSEGTIVKQPVQNIILMIGWHGPSIGKDDAATYAADVFSFILRQPDS
                     RFQRALADTGLVTGANIGYYTQRNVGPINVVAQTTPEKARAALRAIYNEIAHFNDPDY
                     YTDEQLETAKSLLESDDMYSREKLSEYTHTISFWWASTGIDYFRSYHANLRRTSRADI
                     SRYVTTYIQGKPHVGLALMSEESARASQLTPEDLTGEAKAAGASASRR"
     gene            complement(3333..>3805)
                     /locus_tag="VF240_14200"
     CDS             complement(3333..>3805)
                     /locus_tag="VF240_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176412.1"
                     /translation="RIASAVSRTGRVADARFLRVAGAGRAVAAIHDDGAAGGTHLFVL
                     EGRGERFRVTGRSALDADGFRDARWTQEAVDLDSDGYHEVLCTGVLARGTTDARRYVL
                     YSPRTRQTYTLSVAPNTRGARQIKWSPNALTRAAHPFRAALQHRASMALPSRIL"
BASE COUNT          568 a         1111 c         1446 g          680 t
ORIGIN      
        1 ggcgcatctt ttcttttcgg ggcctgaaga ttgaagattg aggcgtcagc cgccagattg
       61 cggtgtgaag atggttttgt ccacgctctt ggggtcgccg atgacgacga agcggatgtt
      121 gcggatgtag tttttggcga cgcgctgcac gtcggccggc gtgacggcgc ggaggcggtc
      181 gaggaagctg aaggcgttgc gccagccgcc gccgagcagc tcatactgag ccagctcgcc
      241 ggcctgcgcg gcgttggtct cctgagcgag gtagtggcgc gtcaggaagt gctggacggt
      301 gccctgtagc tcgtcggccg aaatcggctc gcgctggaga cgcgcgatct cttcgagcat
      361 gaggcggacg gactggttgg cgtccgtcga ggtcacgtag atgccgccga cgttggcgcc
      421 ctgactgtag aggaacgagt cgggcgcgta ggagaggttg cgcttgtagc gcacctcgac
      481 gacgacgcgg ctctggagaa tcgacgacgc gatacgcatc gggtagatgt cttctgacgt
      541 gagcggcggc gcggagaaga cgccctgcac gtagttcgtg ggcagcgctc gctcagtcac
      601 ctcgacgctg ccggcggcga aggagagctg cggcagggcg acggggcggt aatcgccgcg
      661 cggcagcttc ccgaaagtcg cggcgacgcg ctcgcggatc atctgcgggt ctacgtcgcc
      721 gacgaggatg agcagcagcc gcgaggtctg catggcgcgc tcgtgatagc ggcgcacgtc
      781 ggcgagcgtg aggcgcgaga tcgtctcgga gttgccgcgc gggtcgttca ggtacgggtg
      841 gccggcgtag gcggcgcgcg cctgcaagac ttgcaggtag gagtcggggg tgtcggcttc
      901 gttgcgcatg gagacggcga cgcggttgcg gacgcgctcg aagtcctcgg ggacgaagga
      961 gggccgcagg gccacgtcgg tgaaaatctc ccacgagcgg tcgaagttga aacgcgtcgt
     1021 gttgagcgtg agcgcggagt agtcgtagtt gacgccgttg cctagccccg tgcccatccg
     1081 cgccagctcc ttgcgtagcc gctcgcgcgg gaagttgcgg ctggcctcgg tcgccacgtc
     1141 gagcatcaga gactcgacgc ccgcgttggc ggccgtgacg ttttgcgcgc cgccgcgcag
     1201 gaagagaccg gcgaccaccg tctggctgtt ggcgcgccgc ttgaccagaa ccttgaggcc
     1261 gcccacttca aactccgtga cctgcgcggc ggcgtcggcg gaagacacgg cccggttcgc
     1321 cgcggcgggc gctggactcg ccgccgtcgc gtcgcccgcc gtcgcgggcg tcggttgcgg
     1381 tggcggcgac tgctgcgacg cggccggagc ggcggcgagc aggagcgcga gacagagcgc
     1441 ggaagcccgg cgcaagggtc gctcgaaaat aattcccatt ctgttctcca acgtgtttgc
     1501 tgtcgttgct gtcgtgattg ccgtttaaga gtcgccgcga ttacggaacg ggagccggcg
     1561 cgcgcctcac ctgcgcgacg cgctcgcccc ggccgccttc gcctcgccgg tcaggtcttc
     1621 cggcgtcagt tgcgaggcgc gcgccgattc ctcggacatc agtgcgaggc cgacgtgcgg
     1681 cttgccctga atgtaggtgg tgacgtagcg gctgatgtcg gcgcgcgagg tgcggcgcag
     1741 gttcgcgtgg tagctgcgga agtagtcgat gcctgtggag gcccaccaga agctgatggt
     1801 gtgcgtgtac tccgagagct tctcgcggct gtacatgtcg tcggattcga ggagcgactt
     1861 ggccgtctcc agttgctcgt ccgtgtagta gtcggggtcg ttgaagtggg cgatctcgtt
     1921 gtagatggcg cgcagcgcgg cgcgggcctt ctcaggcgtg gtctgcgcga cgacgttgat
     1981 ggggccgacg ttgcgctgcg tgtagtagcc gatgttggcg ccggtgacga ggcccgtgtc
     2041 ggcgagcgcg cgctggaagc gcgagtcggg ctgtcggagg atgaaggaga aaacgtcggc
     2101 cgcgtaagtc gcggcgtcgt ccttgccgat ggaggggccg tgccagccga tcatcaggat
     2161 gatgttctgg acgggctgct taacgatggt gccctcgctg cgttcgagcg gcgggtgctc
     2221 gaccggcggg aactcctcga acgggttccg cgcgcggcgc ggccagccgc cgaagagttc
     2281 ctcggcgaga cgataggcgt cgtccggctt cacgtcgccg gtgacgacga tggccgagtt
     2341 gttcggcacg tagtagcggc cctgaatgag acgcatctgg tcggtcgtcg ccttggagac
     2401 cgtctcgcgg ctgccgcccg gattcttgcg gctcgggtac ttgtagaaga gcttgtcgtt
     2461 catcgccaac tggagatagc cgaacgggtt cgactcgtgg cggtcaagct cgccgatgac
     2521 gactgcgcgc tcgcgctcga actgattggg gtcgaagagc gggtagagcg cggcgtcgcg
     2581 caggaagcgc atggcgacgg ggaagttggg cgtggtggtg gtgaagtagt agttgaccag
     2641 ctcctcctgc gtctgcccgt tgtagatgat gccgagctgg tcgatggtct tcagatactc
     2701 ggcgccgtcg cgggccgcgc ggttggcctt gaagatcatg tgctcgtaga gatgcgacag
     2761 cccgttgagt tcgggcggct cggtatagga gccgttcttg acggccagct cgaccgtgac
     2821 gagcggcacc gagtggtctt cgagcacgat gatctccagc ccgttcggca gcacgcggtt
     2881 gacgtactgc gcctgctgga acacgggcgc gcgctgctgc tgctgctgcg cggcggggtt
     2941 ggcggccgga ttgttcggtg cgggccgtgc ggccggctgt cggcgctgag cggcaaccgg
     3001 cgcggcaaac gtgcccgcgg ccattgtcag ggcgagcgcg aagacaaaga gttggcgttt
     3061 caacggtatc cccttttgga aaatcagcat tgccgtggaa tcacccgcgc gcgcgataac
     3121 ctctggggcg aggcggcagt acggctcgga atggattcgc tgagctttga ggtcgatgat
     3181 agcacgcagg gttgctcgcc tcgcaatagc ggcccgacga gcagacacgg ttcggccatg
     3241 tagatgtaaa agaacgcggc cgacgaattg cccggttcaa ttcgtcggcc gcgttctttt
     3301 acatctacga cgctatcgcg ctgactgacg gcttacaaaa ttcttgacgg gagggccatc
     3361 gacgcgcggt gttggagggc ggcgcggaag gggtgggccg cgcgggtcag ggcgttgggc
     3421 gaccacttga tctggcgcgc gccgcgtgtg ttgggcgcga ccgagagggt gtaggtctgc
     3481 cgtgtgcgcg gggagtagag cacgtagcgg cgggcgtccg tcgtgccgcg cgcgagcacg
     3541 cccgtgcaga gcacttcgtg gtagccgtcg ctgtcgaggt caacggcctc ctgcgtccag
     3601 cgggcatcgc ggaagccgtc ggcgtcgagc gcggagcgcc ccgtgacgcg gaagcgttcg
     3661 ccccggccct ccaagacgaa caggtgtgtc ccgcccgcgg ccccgtcgtc gtggatggcg
     3721 gcgacggccc gcccggcgcc cgcgacccgg aggaatcggg cgtcggcgac gcggcccgtg
     3781 cgcgagaccg ccgaggcgat gcgta
//
LOCUS       DASVES010000347         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_633276, whole genome shotgun sequence.
ACCESSION   DASVES010000347 DASVES010000000
VERSION     DASVES010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633276"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VF240_14205"
     CDS             complement(<1..344)
                     /locus_tag="VF240_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7176413.1"
                     /translation="MTERIYNFSAGPAVLPLPVLEQAREHMLSLPGVGMSVMEISHRS
                     KTFDEIIGGAESGLRELLGIPDDYHVLFLQGGASLQFSMIPMNLLPEGASADYILTGS
                     WGKKALKEAKKQG"
     gene            complement(496..1704)
                     /locus_tag="VF240_14210"
     CDS             complement(496..1704)
                     /locus_tag="VF240_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HEX7176414.1"
                     /translation="MRVLIADKFEQSGRDGLDAAGCDYSYQPDVTGDALVEAIRAYGP
                     EVLVVRSTKVTEPMFDAGPLKLVVRAGAGYNTIDVAAASRRGVYVSNCPGKNAVAVAE
                     LAFALMLALDRRIADNVAELRAGRWNKKEFSKARGLLGRTLGLVGTGQIGREMGARAR
                     AFGMRVVAWSRSLTEERAGALGVERMDSPLDVARASDVVSVHLALTPDTRGIINDEFF
                     AAMREGAFLINTARGEVVDQPALLRAMREKGIRAGLDVYAAEPTSSSGEFTDEIAREP
                     NLYGTHHIGASTDQAQEAIAAETVRIIRAFKETGEVPNVVNLAARTPATHTLVVRHRD
                     RPGVLASVLDSIKAADINVQEMENIVFEGAEAAVARINLDAAPSADALEQLKAANADI
                     IEISLIELRD"
     gene            complement(1895..3130)
                     /locus_tag="VF240_14215"
     CDS             complement(1895..3130)
                     /locus_tag="VF240_14215"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX7176415.1"
                     /translation="MAVIHPFRALRPPADKVSEVAAVPYDVVNSEEARALAADKPHSF
                     LHVSRPEIDLSAGADLYADATYQKAAENFQKLIASAPLETEAEPRLYLYRLRMGDHEQ
                     TGVAACVSVDEYDAGLIRKHEKTRPDKENDRTRHLLELRAQTGPVFLTYRTDRRIDAL
                     VEGEAQSAPLYDFEAEDGVRHTVWRVSAPEPLVRRFAEVPMLYIADGHHRAASAGRAR
                     AALRDANPGHTGREEYNRFLAVVFSADQMQILPYNRAVKDLNGRTPEDFLAAVREKFD
                     VTDDANPRGPGRRGHFHMYLGGRWYGLTPTGSSSAAVSNDPVSSLDVSVLQDNLLDPV
                     LGIKDVRTDKRIDFVGGIRGTEELVRLVDEGRAAVAFALYPTSIEDLIRVSDAGSVMP
                     PKSTWFEPKLRDGILIHSV"
BASE COUNT          527 a          985 c         1187 g          621 t
ORIGIN      
        1 ccctgcttct tcgcctcctt gagcgccttc ttgccccaac tgccggtgag aatgtagtcg
       61 gccgacgcgc cctccggcag caggttcatc gggatcatcg agaactggag actcgcgccg
      121 ccctggagga aaagcacgtg atagtcgtcg gggatgccga gcagctcgcg caggccgctc
      181 tccgcgccgc cgatgatctc gtcgaaggtc ttcgagcggt ggctgatctc catcacgctc
      241 atgccgacgc cgggcaagct cagcatgtgc tctcgcgcct gttcgagcac gggcaggggc
      301 aacacggccg ggccggcgct gaagttatag attctctcgg tcatgtgtct ctcctggaaa
      361 aaaacgtcaa tcgtgaatcg taaatcgtaa atcgtgaaat ggaggttgaa cctgactcag
      421 cttgaatgtc caatcgaagg ccggttgatg ttcaactcgt tcgattcacg attcacgatt
      481 gacgattcac ggcctttaat ccctgagttc gatgagactg atctcaatga tgtcggcgtt
      541 ggcggccttc aattgttcga gcgcgtcggc gctcggcgcg gcgtcgaggt tgatgcgggc
      601 gacggcggcc tcggcgccct cgaagacgat gttctccatc tcctgcacgt tgatgtcggc
      661 ggccttgatg ctgtcgagca cggaggcgag cacgccgggg cggtcgcggt ggcggacgac
      721 gagcgtgtgc gtggcgggcg tgcgggcggc gaggttgacg acgttgggca cctcgcccgt
      781 ctccttgaag gcgcggatga tgcggacggt ctcggcggcg atggcctcct gcgcctgatc
      841 ggtcgaagcg ccgatgtggt gcgtgccgta gaggttcggc tcgcgcgcga tctcgtcggt
      901 gaactcgccg gacgaagagg tcggctccgc ggcgtacacg tcgagaccgg cgcggattcc
      961 cttctcacgc atcgcgcgca gcagggccgg ttgatcgacg acctcgccgc gcgccgtgtt
     1021 gatgaggaaa gcgccctcgc gcatcgccgc gaagaactca tcgttgatga tgccgcgcgt
     1081 gtcgggcgtg agcgcgaggt ggacgctcac cacgtctgag gcgcgggcga catcgagggg
     1141 actgtccatg cgttcgacgc cgagagcgcc cgcgcgctcc tcggtcaggc tccggctcca
     1201 cgcgacgacc ctcatgccga aggcgcgtgc gcgcgctccc atctcgcggc cgatctgccc
     1261 tgtgccgacg aggccgagcg tgcgccccag caggccccgc gccttcgaga actccttctt
     1321 gttccaacgc cccgcgcgca gctccgcgac gttgtcggcg atgcggcggt cgagcgcgag
     1381 catcagcgcg aaggccagct ccgccacggc gacggcgttc ttgccggggc agttcgagac
     1441 gtagacgccg cgacgcgagg cggccgccac gtcgatggtg ttgtagccgg cgccggcgcg
     1501 cacgacgagc ttgagggggc cggcgtcgaa catcggctcg gtgactttgg tcgagcggac
     1561 gacgagcacc tcggggccgt aggcgcggat ggcctcgacg agcgcgtcgc ccgtcacgtc
     1621 cggctggtaa gagtagtcgc agccggcggc gtcgaggccg tcgcggcccg actgttcaaa
     1681 tttgtcggcg atgagcacgc gcatctcttc ggtcgcctcc tgcgaaagag ctattagctg
     1741 ttagcttttt tgtcagtcgt cagtcgtcag ttgttagcgt tgggcaactt gtcacttgcg
     1801 tcgcaggctt ctacaggcgt tacgaaagca gctaaccact gaccacggac aaagaacagc
     1861 tcttagctga tagctaacag ctaacagcta acagctaaac gctatgtatc aaaatcccgt
     1921 cgcgcagctt cggctcgaac caggtcgatt tgggcggcat gacggagccg gcgtcggaga
     1981 ctcggatgag gtcttcgatg gaggtcgggt agagggcgaa ggcgaccgcc gcgcggccct
     2041 catcgacgag cctgacgagt tcctccgtgc cgcggatgcc gccgacgaag tcgatgcgtt
     2101 tgtcggtgcg cacgtccttg atgccgagca cggggtcgag caggttgtcc tgaagcacgc
     2161 tcacgtcgag cgaggagacg gggtcgttgc tgacggcggc cgacgagctg cccgtggggg
     2221 tgagtccgta ccagcggccc ccgaggtaca tgtggaagtg gccgcgtcgg ccggggccgc
     2281 gggggttggc gtcgtcggtc acgtcgaact tttcgcggac ggcggcgagg aagtcttcgg
     2341 gcgtgcgtcc gttgaggtct ttgacggcgc ggttgtaggg gagaatctgc atctggtcgg
     2401 cggagaagac gacggcgagg aagcggttgt attcctcgcg ccccgtgtgg ccggggttgg
     2461 cgtcgcggag cgcggcgcgg gcgcggccgg cgctggcggc gcggtggtgg ccgtcggcga
     2521 tgtagagcat gggcacttcg gcgaagcggc ggacgagtgg ttcgggcgcg ctcacgcgcc
     2581 agacggtgtg acgcacgccg tcctcggcct cgaagtcgta gagcggcgcg ctttgcgcct
     2641 cgccctcgac gagcgcgtcg atgcgccggt cggtgcggta ggtgaggaag acggggccgg
     2701 tctgcgcgcg cagctcaagg aggtgacgcg tgcggtcgtt ctccttgtcg ggacgagtct
     2761 tctcgtgctt gcggatgagt cccgcgtcgt actcgtccac tgagacgcaa gccgcgacgc
     2821 ccgtctgctc gtggtcgccc atgcggaggc gatagagata gaggcgcggc tcggcctcgg
     2881 tctccagcgg ggcgctggcg atgagcttct gaaaattctc ggcggccttt tgataggtgg
     2941 cgtcggcgta gaggtctgcg ccggcgctca ggtcgatctc ggggcgcgag acgtggagga
     3001 agctgtgggg cttgtctgcg gcgagagcgc gcgcctcttc gctgttgacc acgtcgtagg
     3061 ggacggcggc gacctcggaa actttgtctg cgggcggacg cagggcgcgg aaaggatgga
     3121 tgacggccac ttgccttttc ctccgttgcg gatcgaaata agtgcggagc tttggaggcc
     3181 taagactatc cgcacacgcc gcatcagggc aagtcgccgg aggcgttgga acacggatga
     3241 gacggattaa gacggatgtg cacggattaa ggattgacgc ggattgtcat ccgtgttaat
     3301 ccgttctcat ccgtctcatc
//
LOCUS       DASVES010000348         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_902675, whole genome shotgun sequence.
ACCESSION   DASVES010000348 DASVES010000000
VERSION     DASVES010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902675"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VF240_14220"
     CDS             complement(<1..774)
                     /locus_tag="VF240_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX7176416.1"
                     /translation="MPHPIPTPFYEDAGRGLRLYRGDALELLKNARSGIFDLIFADPP
                     YFLSNDGITCQAGRMVSVNKGVWDRATTFEEIYDFNKTWLGECRRLLKPNGAIWVTGT
                     SHNIYSVGYALQTLGYKILNDIAWYKVNPPPNLSCRYFTHATETILWARRDPKGKHTF
                     NYAEMKRENRDKQMQSLWHIKPPGQREKRYGKHPTQKPEALLDRIVRASSNPHDLVLD
                     PFCGSGTTGVACARLGRRFIGIDLMVSYLELAVARLSDEV"
     gene            complement(1116..1460)
                     /locus_tag="VF240_14225"
     CDS             complement(1116..1460)
                     /locus_tag="VF240_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176417.1"
                     /translation="MPPTPKRFTRAYGAEMSSINGLDLTCEADEANLMARLISLETAK
                     LQDDTKVTKAVYERDLTTVLGHLIFEEFTTENDANSRAAIHKAKKEDEVCRGKAFIKN
                     KSTNVIVFREQP"
     gene            complement(1482..2462)
                     /locus_tag="VF240_14230"
     CDS             complement(1482..2462)
                     /locus_tag="VF240_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176418.1"
                     /translation="MKKTPLYLLLCVVLLATGCPKYRPPVNFDAKTLAGRINSHFAAK
                     QAQYYNALGSNDPGMARRTRNELIEDALPYVDAAYEDFITGLTVGRDRTNFIADVVEL
                     GTSAAIGITNGERSIQILGVALTAFRGGRRSADLNFYKEQSTPILITKMDGNRAKVRA
                     TIIIREREGVDTYPIGAAVSDIVAYYNAGTLVRAFTELHKDTADQTKTQEDNLQVLKG
                     VPLTMEATQVFRDLSVAASNILTGLNTGVNSADVPTKDAATTKLKKIVSVLEKNTAAA
                     EALKNANVTSTGSDGPTLISALIDIRTAAALVNDNALLNTVNQAIVDNGQ"
BASE COUNT          493 a          794 c          993 g          637 t
ORIGIN      
        1 gacttcgtcg ctcagccgcg caaccgcgag ttcgaggtaa gacaccatta agtcgatgcc
       61 gatgaaccgt cgtccgagcc gcgcgcaggc gacgccggtg gtgccgctgc cgcagaaggg
      121 gtcgaggacg aggtcgtgtg ggttggagga ggcgcggacg atgcggtcga gcagggcctc
      181 gggcttctgc gtcgggtgct tgccgtaacg cttctcgcgc tgaccgggcg gcttgatgtg
      241 ccagagcgac tgcatctgct tgtcgcggtt ctcgcgcttc atctccgcgt agttgaaggt
      301 gtgtttgccc ttggggtcgc gtcgcgccca gaggatggtc tcggtggcgt gcgtgaagta
      361 gcggcacgag aggttgggcg gcgggttgac cttgtaccag gcgatgtcgt tgaggatttt
      421 gtagccgagc gtctggagcg cgtagccgac gctgtagatg ttgtgcgacg tgcccgtcac
      481 ccagatggcg ccgttcggct tgagcaggcg tcggcactcg ccgagccacg tcttgttaaa
      541 atcgtaaatc tcctcgaagg tcgtcgccct gtcccacacg cccttgttga cgctgaccat
      601 ccgcccggcc tgacaggtga tgccgtcgtt ggagaggaag tagggcgggt cggcgaagat
      661 taaatcgaag atgccgctcc gggcattctt cagcagctcc agcgcgtcgc cccggtagag
      721 gcgcaggcca cgcccggcgt cttcgtagaa cggggtcgga atggggtgag gcatttccgc
      781 ttcccacaat actgtgtctg caaactcgga gcctgccgga cgggttgaag acatgaacgc
      841 gcgggcgcgg ccgacccccg cccttaacct cctcgaaagc gtccacgatc taagatcgaa
      901 tccgccggaa cgtcaaggcg cgggccggtc aagtgatcta ctttcagcgg tttgcggcca
      961 cgtgggcagg ctctcgcggg cggggtgaga ggagaatgtt gatatcggcc agcggctcgt
     1021 ggacgccgtg ggctaggtgg aaaaagagag gggcgcggcg gatgacaagc cgccgcgccc
     1081 ctcctgagag gctcgcgtct tggcttctct gccggctagg gctgttcgcg gaagacgatc
     1141 acgttggtgg acttgttctt gatgaaggcc ttgcccctgc acacttcgtc ctccttcttg
     1201 gccttgtgga tggccgcgcg gctgttcgcg tcgttctcgg tcgtgaactc ctcaaaaata
     1261 agatgaccga ggaccgtcgt gaggtctctc tcgtagactg ccttcgtcac cttagtgtcg
     1321 tcttgcagct tcgcggtctc taagctgatg agcctggcca tgaggttcgc ttcatcggcc
     1381 tcacacgtga ggtccaaccc gttgatggaa ctcatctccg cgccgtaagc tctcgtgaat
     1441 ctcttgggag tgggtggcat gtttgctctt cctttctggc gctactggcc gttatctacg
     1501 atggcttggt tgaccgtgtt cagcagggcg ttgtcgttga cgagggcggc cgccgtcctg
     1561 atgtcaatca gagcagagat cagggtcggg ccgtctgagc ctgtcgaggt gacgttggcg
     1621 ttctttaaag cctcggcggc ggctgtgttt ttctccagca ccgacacgat ctttttcagc
     1681 ttcgtcgtgg ccgcgtcctt cgtcggcacg tcggcgctgt tcacgccggt gttgaggccg
     1741 gtgaggatgt tgctggccgc gaccgaaagg tcgcggaaca cttgcgtggc ctccatggtc
     1801 aggggcacgc ccttgagcac ctgtaagtta tcttcctgcg ttttcgtctg atccgcggtg
     1861 tctttgtgca gctcagtgaa ggcccgcacg agagtacccg cgttgtagta ggcgacgatg
     1921 tcggaaacgg ccgccccgat ggggtaggtg tccacgccct ctctttcgcg gatgatgatg
     1981 gtggcgcgca ctttggcgcg gttgccgtcc atcttggtga tcaggatggg agtagattgc
     2041 tccttgtaaa aattgaggtc ggctgaccga cgcccgcccc tgaaggcggt gagggcgacg
     2101 cctagaatct ggatggagcg ctcgccgttc gtgatgccga tggcagccga cgtacccagc
     2161 tcgacgacgt cggcgatgaa gttcgtgcgg tcgcgcccga cggtcaggcc ggtaatgaaa
     2221 tcctcgtagg cggcgtcaac gtagggtagt gcgtcttcaa taagttcgtt ccttgttctc
     2281 ctcgccatgc cggggtcgtt gctcccgaga gcgttgtaat attgcgcctg cttggcggcg
     2341 aagtgactgt tgattcgacc ggcaagggtc ttggcgtcga aattcacggg aggtctgtat
     2401 ttcgggcagc cggtggcgag taacacgacg cagagcaaca ggtagagggg ggtcttcttc
     2461 atggtcttgc ctctgtttca ggagtcaggt gaaattgcca aagccgaggg gatcgtctcg
     2521 gcccagagtc ccgagggaaa agtggttaga agtctgacac ggaacttgcg gccgtagagt
     2581 cggccacagc gctagagccc gcgcgaagcg gctgcgttaa taccaacttg attgaaaaat
     2641 agcaatactt attttcgccg tcgcgggcgc tccgcggtca accccgccag acgagaaagg
     2701 gacggcccgt tgcacgtctc taacctttaa tgacggagcg cggcaaaacg tttcagcgaa
     2761 ccgtgcggaa attttcaggg cgagagaaat cgggtgtggt gtcagaccgc ggcgcgtaag
     2821 gattgggcga tgagggggac gatgcggcgg gggacggcga tggcttcttt gatgtggcgg
     2881 gcgtgggtga agtggccggc gacgtggagg cccgaga
//
LOCUS       DASVES010000349        14738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1410203, whole genome shotgun sequence.
ACCESSION   DASVES010000349 DASVES010000000
VERSION     DASVES010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410203"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..643)
                     /locus_tag="VF240_14235"
     CDS             complement(68..643)
                     /locus_tag="VF240_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176419.1"
                     /translation="MPDDPIRASRLGILEKLGLTERRLSGLKVRELTDNQRIKIIGTD
                     LELTTSSITLTPGKPRIGKSHLTFLTPVLVLTEELASNNVALFTSSVPGATFPSVQIE
                     FPQIKKNKPHLVEFNVSLFDKSKTYKFRVFHFPLGGFQDISLTQTQVITALVPAIPNF
                     SGDLGASIQQRNTVGENIGWSLHSVRVTSIS"
     gene            957..2486
                     /gene="ffh"
                     /locus_tag="VF240_14240"
     CDS             957..2486
                     /gene="ffh"
                     /locus_tag="VF240_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7176420.1"
                     /translation="MFESLSDKLKRALKNLRGEGVLTPEHVDAALREIRMALLEADVN
                     YKVAKDFIASVKEKAEGQHVWQELKPSEQVVKIVYDELVSLLGGTSSRLVFTKQAPNV
                     VMIVGLQGSGKTTSTGKIAMWLAKNQERKPLLVSTDVNRPAAREQLRVIAKATGQSIF
                     EKPESNDPVELAREAFRHAQQTGYDTLLIDTAGRLHVDDELMEELVRIKGETRPVEIL
                     FVADAMTGQDAVRSGEEFHRRVGVTGVVLTKMDGDARGGAALSIKQVTGQPVKFVGVG
                     EKYDALEPFYPERIAQRILGMGDVLSLIEQVQDKVDQDEAAEQIKKLQKNEFSLEDFR
                     GQLRQVKRLGSFSKIMKLLPDQLLGGMGMPELNEEQSEQMEKELKRTEAIIDSMTRQE
                     RNDHRLLNAGRRRRIAMGSGVTVADVNQLVKQYVEMRQMMKQFSGMGLFGGGGGGGGG
                     MKNRMMRRMAGMMGMPDVSGMMGGGGAAGEDGDAGALPAAPGSGRKKIRKKPRHKKRR
                     G"
     gene            2574..2858
                     /gene="rpsP"
                     /locus_tag="VF240_14245"
     CDS             2574..2858
                     /gene="rpsP"
                     /locus_tag="VF240_14245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7176421.1"
                     /translation="MLAIRLMRMGAKKAPSYRVIVKEKLSKRDGAYIEKVGFYDPTRN
                     PAEVRLDMDRVNYWVSKGAQPTDTVRQLIKTFARTQQAASAESDTAASGA"
     gene            2888..3121
                     /locus_tag="VF240_14250"
     CDS             2888..3121
                     /locus_tag="VF240_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX7176422.1"
                     /translation="MKEAVELIVKALAHDPESVDVRELNRDQRTTVIEVRVAQGDMGK
                     VIGRQGRTIKALRVLLHAAGMKHNHRYVLEVVE"
     gene            3118..3678
                     /gene="rimM"
                     /locus_tag="VF240_14255"
     CDS             3118..3678
                     /gene="rimM"
                     /locus_tag="VF240_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7176423.1"
                     /translation="MSGARDDDGGVDWETVELLAVARVAKTRGVRGEVVANLLTDFPE
                     RFDRLEELVAVGPAGERELLVIENHWFHGPRVVFKFEGYDAPETAQVLVGRELAVPES
                     EAVELEEGAFYDWQLAGCRVETTEGQDLGAVRELLRAGGDAPLLVVRDDDGRERLIPF
                     AESICVEVDIDARLIRVDPPDGLLEL"
     gene            3764..4507
                     /gene="trmD"
                     /locus_tag="VF240_14260"
     CDS             3764..4507
                     /gene="trmD"
                     /locus_tag="VF240_14260"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7176424.1"
                     /translation="MRFDILTIFPDFFGAVFDYGIVRRALSAGLVEVEAHDLRRWTHD
                     RHKIVDDRPFGGGDGMVLKPEPIFEAVESLAGAGEARAFREAGRRVVLLSPQGRQLTQ
                     ELAAELATCEQVVVICGRYEGVDERVSEALVTDEVSIGDYVLSGGEPAAVVLVDAIVR
                     LIPGALGSETSAVNESFAGGLLDFPHYTRPPDFRGLRVPEILLGGHHAEIARWRRRRA
                     LEKTRRNRPDLFEKLELSADELRELDEPE"
     gene            4640..5038
                     /gene="rplS"
                     /locus_tag="VF240_14265"
     CDS             4640..5038
                     /gene="rplS"
                     /locus_tag="VF240_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX7176425.1"
                     /translation="MNRLDMIEKPQMRADIPAMEPGDTVRVHVRIKESETKERLQVFE
                     GVVIALKGGGVRETVTVRKTSFGVGVERIFPVHAPVIGKIELIRHGRVRRAKLYYLRE
                     LRGKAARIRERDTRPAGHAKGATASNKGNK"
     gene            5137..5781
                     /locus_tag="VF240_14270"
     CDS             5137..5781
                     /locus_tag="VF240_14270"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7176426.1"
                     /translation="MPRRLSSICTTFEEEARSRGHRLVAGVDEVGRGALAGPVVASAV
                     ILDPEAPLPAGLDDSKKLTPLQRERIAGELRQTVVCYAVGVIYPDEIDATNILIATRR
                     AMTEALKGLRPCAEFLLIDAVQLRESALPQRAIIHGDSISASIAAASVLAKTFRDRLM
                     REYDSLHPGYNFSRHVGYGTREHYEAIRLRGPCAIHRRTFRGVLPETDSAPAGQ"
     gene            complement(5825..6220)
                     /locus_tag="VF240_14275"
     CDS             complement(5825..6220)
                     /locus_tag="VF240_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176427.1"
                     /translation="MEIIFEILFEVVFEFILQVFGELLFEVGLRSISEPFKDRETRSP
                     VLAFVGYSLAGAAMGGLSLLIFPGPLVRGVGFRGGSLIVSPLLAGAAMTGLGWLRRRQ
                     GKRLLRLDKFAYGAVFAFGMALVRFLFTS"
     gene            complement(6413..6868)
                     /locus_tag="VF240_14280"
     CDS             complement(6413..6868)
                     /locus_tag="VF240_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX7176428.1"
                     /translation="MLTRLLRFGHTGADVKHVQERLNILGAYFTIYGMEPPSRHPQLE
                     MDGIFGPKTFTRVLEFQGQERLDPDGIVGPITYGRFREIGVRESVSVPSDPPITIILF
                     RPIGSRLTEVLHMLHEDRLTKILPRSSLFLRETVRLGDPWPIIPVPNNM"
     gene            7144..8268
                     /locus_tag="VF240_14285"
     CDS             7144..8268
                     /locus_tag="VF240_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7176429.1"
                     /translation="MHIAIDAHSVGTGLAGNETYAANLVESLAAVDGDNRYTLYVTKE
                     QAVNRFSGRWPNFEVRRTLPHSPLVRVPLTLSRELRRRPVDVLHVQYTAPPFAPCPVV
                     ATIHDLSFEHLPETFKRRSWMQLRLTVRRTARRARHVIAPSEYTRADLIKTYGLRPER
                     VSVTPLAASPHFRPVREATEIERVCNLYGIRGSYILGVGSIQPRKNLARLIEAYADLR
                     RAGFGDKLPQLVLVGRQAWLYGETLRAVTRERVAHRTIFTGYVSEDDLPALYTGALCF
                     AYPSYFEGFGLPVLEAMACGAPVLAGDRTSLPEVVGDAGLLVDPFDTGAIARSLARLI
                     EDAELRAALRVKGLERARQFDWRETARSTLGIYRSATEEE"
     gene            8348..9523
                     /locus_tag="VF240_14290"
     CDS             8348..9523
                     /locus_tag="VF240_14290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7176430.1"
                     /translation="MRIAILGTRGVPASYGGFETFAEQLSTRLVARGHEVTVYCRAHY
                     VSPRQLEYKGVRLKVLPTIRHKYLDTVVHAFLSSLHAAGAGRFDAALICNAANAPIAP
                     VLRASGTPVAINVDGLEHKRRKWNWFGRNYYRLAERLAVWLPNETVTDARVIQDYYLT
                     RYSARSTMIAYGAEVERRLDRAAVRQWGVEPNRYVLYVSRLEPENNAEMVIEAFKRVR
                     TPHKLLIVGDAPYAHEYIQNLRAHARRDRRIIFTGFVFGDAYRALQQNAYLYVHATEV
                     GGTHPALLEAMGYGNCVLTLDTPENLEAVGDAAIPYADAKDLESKLQRVLRDGAIVST
                     YRRRAQDRVRELYDWDYVVDRYEDLFARMAGLPTPRATEAPAREETVKHEESAVKKV"
     gene            complement(9605..10453)
                     /locus_tag="VF240_14295"
     CDS             complement(9605..10453)
                     /locus_tag="VF240_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176431.1"
                     /translation="MIKKSSPPFARACLAALFVFAAAGVALSQAGQSPAQSPAPPSVK
                     ASPPPADERAEAVIKRAVEALGGRAFLDVRTVVSSGYFTPYKDGVATLPIRFTDYLAF
                     PDRERTEFRGGGVRSIQTYSGDRGWLLDGMKRSINDATPEQVGDFRLAMRTSLDNVLR
                     GWWRTGGAKIEHAGRREAGLGKRNEVVRLSYPDGFAVEFEFGARDGLPAKVLYRKKNS
                     EGEEVEEEDRYAQILSVGGVSAPFVIDHFRAGTQSSRINYEKVDFNQPVPDSLFERPA
                     DAKAIK"
     gene            complement(10600..12885)
                     /locus_tag="VF240_14300"
     CDS             complement(10600..12885)
                     /locus_tag="VF240_14300"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX7176432.1"
                     /translation="MVDLGTYKDRFAESGQRVFERAVQESRNRDQNYIAVEHILSALA
                     NEEDELFNSMMRDLSLDPRSVKVLIEKRLEAARYQNVAKGIRIAPETIDLFKRAMERA
                     RAQGRKTIEATDLFMALSQDERSVFVGVLRNLGASPESIVEQVRARVRTREQEDEQYK
                     KKFELPPYLKHFGVSLNRLARADKIPPTIGREMEIQQMIEILCHRERANSPMLVGEPG
                     VGKTAVVEGLARLVELEPEKVPARLRNSHIVQLQMGGLVAGTMLRGMFEERIKGIIDE
                     VKESEDLILFIDEAHTIIGAGAALGTSSDAANMFKSALARGEMRIIGATTNTEFKEYI
                     AEDEALARRFRQVKVAEPTISETREILFGLRPRLEKNYSVTISDDAINTALEMAPKYI
                     RNLHLPDKAIGWLDTAAVKVEINEPDAMVVRPEHIIDVISQESRIPRDMIFRDTTDRF
                     SNMEDELGHRVIGQKEAVHSVAQRLRLNKGPLKESHYKPDGVLLFLGPTGVGKTELAK
                     AVAESMFGDDEKMVRIDMSEYMDGTIGIEKLIGMPRGIVGSERGGILTEQLRENPYTV
                     LLLDEVEKASPTLMNLFLQAFDEGWLTDGRGKKVYLSDAIVIMTSNLGSENFKKYEKP
                     LGFGTKTLGDMKAIKGDVMKAAETRFSAEFRNRIDEIVIFSPLTMDEVREIARLYLGR
                     LRAQMEHQGKVLDITEEAINLLTEKGFSTTYGARFLKRHIDEKVKLPITAMWKTAPRF
                     VVDVREGEVTIDAAEDAAEVS"
     gene            complement(13233..>14738)
                     /gene="groL"
                     /locus_tag="VF240_14305"
     CDS             complement(13233..>14738)
                     /gene="groL"
                     /locus_tag="VF240_14305"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7176433.1"
                     /translation="KFGSPVITKDGVTVAKEIQLKDNYENMGAQMVREVASKTNDIAG
                     DGTTTATVLAQAIFREGVKNVTAGANPMLLQRGLQRGVEAAVKALEKMSKTVKDKSDL
                     ANVAAVSANNDREIGDLIAEAMEKVGRDGVVTVEESRTLQTELDLVEGMQFDRGYLSP
                     YFVTNADRMECVLDDPLILIHEKKVAAMRDLLPLLEQVLQKGRSLLIIAEDVEGDALA
                     TLVVNRLRGTVKVCAVKAPAFGDRRKAILEDLATLTGGRLITEDIGVKLESVTLEDLG
                     TAKRVIIDKDSTTVVEGAGEPRAIEGRVQAIRRQMDETTSDYDREKLQERLAKLVGGV
                     AVIRVGAATETEMKEKKMRVDDAVNATRAAAQEGIVPGGGVALVRAARALDKLDAEGD
                     ELTGIRLLKRAMQEPLRRIAENAGVDGSIAINVVEESKGKTGFNAATGKYEDLASAGI
                     IDPTKVVRTALQMAVSVAGLLLTTDAAVTELPEPKRAAAPQMGGGEDYGDY"
BASE COUNT         2610 a         4691 c         4739 g         2698 t
ORIGIN      
        1 ttctccgcct cggtcgccgc cttcggcatg ctgcttcggc gactcgaagc acaaggaggg
       61 gacgagacta actgatggag gtgacgcgca cggaatgaag cgaccagccg atattctcac
      121 cgacggtgtt ccgttgttgg atcgaagccc ccaggtcgcc ggagaagttg gggatggccg
      181 gaaccagagc cgtgatgacc tgcgtctgcg tcagggagat gtcctgaaag ccgccgagcg
      241 ggaagtgaaa gacgcggaac ttgtaggtct tcgacttgtc gaataacgaa acgttaaact
      301 ccaccagatg cggcttgttc ttcttgatct gcgggaactc aatttgtacg ctggggaagg
      361 tcgcgccggg gacggaagag gtgaaaagcg cgacgttgtt gctcgcaagt tcttcagtca
      421 ggacgagcac cggcgtgaga aatgtaaggt ggctcttccc gatgcgcggc ttaccgggcg
      481 tcagggtgat ggagctggta gtcagctcca agtccgtgcc gattatcttt atgcgctggt
      541 tgtcggtcaa ttctctgacc ttcaaaccgc tcagtcgtct ctcagtgagg ccgagtttct
      601 caaggatgcc aagtctggac gctcgaatcg ggtcgtctgg catggtcgcg agtacctccg
      661 ctgtggattt tcgtttcatg ccccaacggg acacggggca aggcgcggcg gattctatct
      721 ccgtccccct cctttagcaa tctattttgc ccgcctctat cgtgatcttc cgccgtcgcg
      781 gggacgcttc tctagtcaaa agtgttccgc ctcgcgacga cgaagcagaa tcgcctccgt
      841 cttacgccgc gggtcgctcc ccacgccggc cccgagcgcg tagtcccttt ccttgacagt
      901 atttaccggc gtcgctatca tccttgtttt cggctggggc cggccgtcgt gtctgtatgt
      961 tcgagtcttt atccgacaag ctgaagcggg cgctgaagaa tctgcgcggc gagggcgttt
     1021 tgacgccgga gcacgtggac gccgccctgc gcgagattcg gatggcgctc cttgaggccg
     1081 acgttaacta caaggtcgcc aaggacttca tcgcgtcggt caaggagaag gccgaaggcc
     1141 agcacgtctg gcaggagctg aagccctcgg agcaggtcgt caagatcgtc tacgacgaac
     1201 tcgtctccct gctcggcgga acctcgtcgc gcctcgtctt caccaagcag gcgccgaacg
     1261 tcgtgatgat cgtcggcctg caaggctcgg gcaagacgac ctcgacgggc aagatcgcca
     1321 tgtggctggc gaagaaccag gagcgcaagc cgcttctggt ctcgacggac gtgaaccgac
     1381 ccgcggcgcg cgagcagttg agggtgattg cgaaggcgac gggacagtcg atctttgaaa
     1441 agcccgagtc gaacgacccc gtggagttgg cgcgcgaagc cttccgccac gcgcagcaga
     1501 cgggttacga cacgctgctc atcgacacgg ccggacgcct ccacgttgac gacgagttga
     1561 tggaggagct ggtcaggatc aagggcgaga cgcgccccgt cgagattctg ttcgtcgccg
     1621 acgcgatgac tggtcaggac gccgtgcgct cgggcgaaga gtttcatcgg cgggtcggcg
     1681 tgacgggtgt ggtcttaacc aagatggacg gcgacgcgcg cggcggcgcg gccctctcca
     1741 tcaagcaggt gacggggcag cccgtgaagt tcgtcggcgt cggcgagaag tatgacgccc
     1801 ttgagccgtt ctatcccgag cgcatcgcgc agcgcatcct cggcatgggc gacgtgctgt
     1861 cgctgatcga gcaggtgcag gacaaggtcg atcaggacga ggccgccgag cagatcaaga
     1921 agctccagaa gaatgagttc tcgctcgaag acttccgcgg ccaactccgg caggtcaagc
     1981 gcctcggctc gttctcgaag atcatgaagc ttttgcccga ccagctcctc ggcggcatgg
     2041 ggatgcccga gctgaacgag gagcagtccg agcagatgga gaaggaactc aaacgcaccg
     2101 aggcgatcat cgactcgatg acgcgacagg agcgcaacga ccaccggctc ctcaacgccg
     2161 gccgccgccg acgcatcgcg atggggtcgg gcgtcaccgt ggccgacgtt aaccagctcg
     2221 tcaagcagta cgtcgagatg cgccagatga tgaagcagtt ttcggggatg ggcctcttcg
     2281 gcggcggcgg tggtggcgga ggcgggatga agaaccggat gatgcgccgc atggccggca
     2341 tgatgggcat gcccgacgtg agcggcatga tgggtggcgg cggcgccgcg ggcgaagacg
     2401 gagacgccgg cgcgttgccg gccgcccccg gctccggccg caagaaaatc agaaagaaac
     2461 caagacacaa aaaacggaga ggttagaggt tagaggttag aggttagtga aagaccgaaa
     2521 tgctttttac tgacctctga cctctgacct ctgacctctc aaccggaggt tgaatgttag
     2581 caatcagact catgcgtatg ggcgcgaaga aggcgccgtc gtaccgcgtc atcgtcaagg
     2641 aaaagctgtc gaagcgcgac ggcgcttaca tcgagaaggt cggcttctac gacccgacgc
     2701 gcaaccccgc cgaggtgcgc ctcgacatgg atcgcgtcaa ctactgggtg tcgaagggcg
     2761 cgcagccgac cgacaccgtc cggcagttga ttaagacttt tgcccgcacg caacaggccg
     2821 cgtcggccga gtccgacacg gcggcgtccg gcgcgtaaag aagttgtccg tggactgctg
     2881 agcgaagatg aaggaagccg tcgagttgat cgtcaaggcg ctggcgcacg accccgagtc
     2941 ggtggacgtg cgcgagctga atcgcgacca gaggacgacc gtcatcgagg tgcgcgtcgc
     3001 gcagggcgac atgggcaagg tcatcgggcg gcagggccgc accatcaagg ccctgcgcgt
     3061 gctgctccac gccgccggca tgaagcacaa ccaccgctac gtcctcgaag tcgtcgaatg
     3121 agcggcgcga gggacgacga cggcggcgtc gattgggaga cggttgagct gttggccgtc
     3181 gcgcgcgtgg cgaagacgcg cggggtgcgc ggcgaggtcg tcgcaaacct tctgaccgac
     3241 ttccccgagc gcttcgaccg gctcgaagag ttggtcgccg tcggccccgc gggcgagcgc
     3301 gagttgctgg tgattgaaaa ccactggttc cacggcccgc gcgtcgtctt caagttcgag
     3361 ggctacgacg cgccggagac ggcccaagtg ctcgtcggtc gtgagctggc cgtccccgag
     3421 tcggaggcgg tcgaactcga agagggcgca ttctacgact ggcagctcgc cggctgccgc
     3481 gtcgagacga ccgaggggca agacctcggc gccgttcgcg aactgcttcg cgccggcggc
     3541 gacgccccgc tcctcgtcgt gcgagacgac gacggcaggg agcgcctgat acccttcgcc
     3601 gagagcatct gcgtcgaggt ggacatcgac gcccggctga ttcgggtcga tccgccggac
     3661 gggctgttgg aactatagag gtcagaggct agaggttaga ggtcagtaaa aacttttcgg
     3721 tctttcacta acctctgacc tctaacctct gacatctgta gttatgcgtt ttgacatcct
     3781 gacgatcttc ccggactttt tcggcgcggt cttcgactac gggattgtgc ggcgcgcgct
     3841 ctcggccggg ctggtcgagg tcgaggcgca cgacctgcgg cgctggacgc acgaccggca
     3901 caagatcgtg gacgaccgcc ccttcggcgg gggcgacggg atggttctca agccggagcc
     3961 gatctttgag gcagtggaat ctttggccgg ggccggcgag gcgagggcgt ttcgcgaggc
     4021 gggccgccgc gtcgtgctgc tgtcgccgca ggggcgacag cttacacagg agttggccgc
     4081 cgaactcgcg acgtgcgagc aggtcgtcgt catctgcggg cgctacgagg gcgtggacga
     4141 gcgcgtctcc gaggcgctgg tgaccgatga ggtctccatc ggcgattacg tgctgtcggg
     4201 cggcgagccg gcggccgtcg tcttggtgga cgcgattgta aggctcatcc ccggcgctct
     4261 ggggagcgag acctcggccg tcaacgagtc gttcgccggg gggctgctcg actttccgca
     4321 ttacacgcgc ccgcccgatt ttcggggcct gcgcgtgccg gagattttgc tcggcggaca
     4381 ccacgccgag atcgcccgct ggcgtcgccg ccgggcgctt gagaagacgc ggcgcaaccg
     4441 ccccgacctc ttcgagaagt tggaactctc cgccgacgag cttcgagaac tcgacgaacc
     4501 ggaatgagat ttgaaaccgc gtgtgagatt tgaaacacgt ttgatgacga caaagccgtc
     4561 agcttccggc gccagcaagg ctgatagcta atagctgaca gttaatagct gaaagccgac
     4621 cgctttttga ggtgaaatta tgaaccgctt agacatgatc gagaagccgc agatgcgcgc
     4681 ggacattccg gcgatggagc cgggcgacac ggtgcgcgta cacgtaagga tcaaggagtc
     4741 ggagacgaag gagcgcttgc aggtgttcga aggcgtggtc atcgccctca agggcggcgg
     4801 cgtgcgcgag accgtgacgg tcaggaagac gagcttcggc gtcggcgtcg agcgcatctt
     4861 ccccgtccac gctcccgtca tcggcaagat cgaactcatc cgccacgggc gcgtccgccg
     4921 cgccaagctc tactacctgc gtgagctgcg cggcaaggcc gcccgcatcc gcgagcgcga
     4981 cacgcgcccg gccggccacg ccaagggcgc caccgcctcc aacaagggca acaagtaaag
     5041 gcaggtgata agtgatgagt gacaagtgat gagaaagaga aagccactgt cttcttcttt
     5101 ctcatcactt atcactcatc actcatcacg tcttttatgc cccggcggct gtcttccatc
     5161 tgcacgacgt tcgaggagga ggcccgctcc cgcgggcacc gtctggtggc tggcgtggac
     5221 gaggtggggc gcggagcgct cgccggcccc gtcgtcgcgt cggccgtcat cctcgaccct
     5281 gaggcgccgc tccccgctgg gctggacgac tcgaagaagc tcacgccctt gcagcgcgag
     5341 cgcatcgccg gcgagctgag acaaacggtt gtctgctacg ctgtcggcgt catctatccc
     5401 gacgagatcg acgcgacgaa cattctcatc gccacgcggc gcgcgatgac agaggcgctc
     5461 aaaggtctcc ggccctgcgc cgagttcctg ctgattgacg ccgtccagct ccgcgagtcc
     5521 gcgctcccgc agcgggccat catccacggc gactccatct cggcctccat cgccgccgcc
     5581 tccgtcctcg ccaagacctt ccgcgaccgc ctgatgcgcg agtacgactc gctccacccc
     5641 ggctacaact tctcgcgcca cgtcggctac ggcacacgcg agcactacga ggccatccgc
     5701 ctgcgcggcc cgtgcgcgat tcaccgccgc acgttccgcg gcgtgctccc ggaaacggat
     5761 tcggcgcccg cggggcagta aagcgctggg cactgttccc ttccaacctc gtgaggcacg
     5821 gccttcaaga cgtgaaaagg aaccgcacca gcgccatacc aaaggcgaag accgccccgt
     5881 aggcgaactt gtcgagccgc agcagacgct tgccctgccg ccgccgcagc cagcccaggc
     5941 ccgtcatcgc ggcccccgcc aaaagcggcg agacgatcag gctgcccccg cgaaagccca
     6001 cgccacggac gagcggcccc ggaaagatga gaaggctcag accgcccatc gccgcgcccg
     6061 cgagcgagta gccgacgaag gccagtacgg ggctgcgcgt ctcccggtcc ttaaacggct
     6121 ccgagatgct ccgcaggccc acctcgaaca acagctcgcc gaacacctgc aagatgaact
     6181 cgaaaacgac ctcgaacagt atctcgaaaa taatttccat cggccggcga tgattctcct
     6241 ccgccaagac tgcgggccgc cgttaactta tgccaacgct ttacgccgcg caacgaacca
     6301 ccgctggact tccgtccccg ccgcacaaac tcattagagc gtgtgagacc gacgactgct
     6361 gccgactgct gcttgcccac ggttgcacac gttggcggcc ggcaatcagc tgttacatat
     6421 tgttcggcac gggaatgatc ggccagggat caccgagccg aacagtctct ctaagaaaca
     6481 acgatgatcg gggcaggatc ttagtcaacc tgtcttcgtg taacatgtgg aggacttcag
     6541 tcaatctgct accaatcgga cgaaacagta taatagtgat gggcgggtcc gacgggacgc
     6601 tcacgctttc acgaacgccg atctcccgga aacggccata ggtaatcggc cccacaatgc
     6661 cgtccgggtc gaggcgctcc tgaccctgaa attcgagtac gcgggtgaac gtcttggggc
     6721 cgaagatacc gtccatctcc aactgcgggt gcctcgacgg cggctccatc ccgtagatcg
     6781 taaaataagc gcccaggatg ttcagcctct cctgaacgtg cttcacgtct gcacccgtat
     6841 ggccgaaccg cagtagtctg gttagcacga gtaacctccc tttcgctcgc tgtgactgga
     6901 tgccaaaagg ctccagcctc atttcgcccg ttactggcgg ccgagtcgtt ggtaacgggg
     6961 cgcagaaagt gaggagctga aggccttcgc cccgcggcca gtcgtcgatt cggagaggat
     7021 gaggcggcct gacattagct tagcttcctg gggtgagtca aaaatgtcag aggccatcgc
     7081 aagagtgtta tcatctgacc agcgctatct aatttgatta ctgggaggag ttgtcgtccg
     7141 cccttgcaca tcgccataga cgcgcactcg gttgggacgg gtctggccgg caacgagacc
     7201 tacgcggcga acctcgtcga gtcgctggcc gccgttgacg gcgacaaccg ctacacgctc
     7261 tacgtgacga aggagcaggc cgtcaaccgc ttcagtgggc gctggcccaa cttcgaggtg
     7321 cgccgcaccc tcccacactc gccgctcgtg cgcgtccccc tgacgctctc aagagagctg
     7381 cgccgccgcc ccgtggacgt gctccacgtc cagtacaccg cgccgccgtt tgcgccctgc
     7441 cccgtcgtcg ccaccatcca cgacctctcc ttcgagcacc tgcccgagac cttcaagcgt
     7501 cggagctgga tgcaactgcg gctcacggtg cggcgcacgg cgcgtcgggc gcgtcacgtc
     7561 atcgccccct cggagtacac ccgcgccgac ctcatcaaaa cttacggcct gcggcccgag
     7621 cgcgtcagcg tcacgccgct ggcggcctcg ccgcacttcc ggcccgtccg cgaagcgaca
     7681 gaaatcgaaa gagtttgcaa tctatatggg attcgcgggt cgtatatcct gggggtcggg
     7741 tcgattcagc cgcggaagaa tctcgcgcgc ctgatcgagg catacgccga cttgcgccgg
     7801 gccggctttg gcgataagct gccgcaactt gttctggtcg ggagacaggc gtggctctac
     7861 ggagagacct tacgcgccgt cacgcgcgag cgggtcgccc atcgcacgat ctttaccggc
     7921 tacgtatcag aagacgacct ccccgcactg tacacggggg cgctctgctt cgcataccca
     7981 tcctacttcg agggcttcgg gctgcccgtg ctggaggcga tggcgtgcgg cgcgcccgtg
     8041 ctggcgggcg accggacgag cctgccggag gtcgtcggcg acgccggcct gctcgtcgat
     8101 ccgttcgaca cgggcgccat cgcgcgctct ctggcgcggc tcatcgaaga cgcggaacta
     8161 cgcgccgccc tgcgcgtgaa aggactcgaa cgcgcacgcc aattcgactg gcgcgagacg
     8221 gcgcgctcga ctctcggaat ctaccggagt gccacggagg aggagtgata agtgatgagt
     8281 gataagtgat aagaaggaag atggcttttt cttatcactt atcactcatc acttatcact
     8341 gtttttgatg cggatagcga ttctgggaac gcggggggtg ccggcgagct acggcgggtt
     8401 cgagaccttc gccgagcagc tctcgacgcg actcgtggcg cgcggccacg aggtgacggt
     8461 ctactgccgc gcgcactacg tctccccgcg acaactcgaa tacaagggcg tccgcctcaa
     8521 agtcctgccc acgatccgcc acaagtatct cgacacggtc gtccacgcct ttctctcgtc
     8581 gctgcacgcc gccggcgccg ggcgcttcga cgccgcgctc atctgtaacg ccgccaacgc
     8641 ccccatcgcc cctgtcctgc gcgcttcggg cacccccgtc gccatcaacg tggacggcct
     8701 cgaacacaag cgccgcaagt ggaactggtt cggccgcaac tattatcgcc tcgccgagcg
     8761 cctcgccgtc tggctgccga acgaaacggt gacggacgcg cgcgtcatcc aggactacta
     8821 cctgacgcgc tactcggcgc gctcgacgat gatcgcctac ggcgcggagg tggagcgccg
     8881 cctcgaccgc gcggccgtgc gccagtgggg agtggagccg aaccgctacg tgctctacgt
     8941 ctcgcgcctc gaacccgaga acaacgccga gatggtcatc gaggctttca agcgcgtgcg
     9001 caccccgcac aagctcctca tcgtcggcga cgcgccctac gcgcacgagt atatccagaa
     9061 cctgcgcgcc cacgcccgcc gcgaccgccg catcatcttc accgggttcg tcttcggcga
     9121 cgcctatcgc gcgctccagc agaacgctta tctctacgtc cacgccaccg aggtcggcgg
     9181 cacgcacccg gcgctgctcg aagcgatggg ctacggcaac tgcgtgctca ccctcgacac
     9241 gcccgaaaat ctggaggccg tcggcgacgc cgccattcct tacgcggacg cgaaagacct
     9301 ggagtcgaag ctccagcgcg tgctgcgcga cggcgcgatt gtgagcacct accgccggcg
     9361 cgcgcaggat cgcgtccggg agttgtacga ctgggactac gtcgttgacc gctacgagga
     9421 tttattcgcg agaatggccg gcctgccgac cccgcgcgcc actgaggctc cggcccgaga
     9481 ggagacggtc aagcacgaag agtcggcggt gaagaaggtc tgaacgcgtg ggcaggggaa
     9541 cacggatcaa acggatgagg acggattcgt atcaatccgt cctcatccgt ttgatccgtg
     9601 ttccttattt gattgctttg gcgtcggccg ggcgctcgaa gagtgagtcg gggacgggtt
     9661 ggttgaaatc gaccttctcg tagttgatgc ggctcgactg cgtgccggcg cggaagtggt
     9721 cgatgacgaa cggcgccgac acgccgccga cgctcaggat ttgcgcgtag cggtcttcct
     9781 cctccacctc ctcgccttcc gagttcttct tccggtagag gactttggcg ggcagcccgt
     9841 ctctggcgcc gaactcgaac tcgaccgcga acccgtccgg gtaagagagg cgcacgacct
     9901 cgttgcgctt gcccagaccc gcctcgcgcc gcccggcgtg ctcgatcttc gcgccgcccg
     9961 tgcgccacca tccgcgcagg acgttgtcga ggctcgtccg catggcgagg cggaagtcgc
    10021 cgacctgttc gggggtggcg tcgttgatgg agcgcttcat gccgtcgagg agccagccgc
    10081 ggtcgccgga gtaggtctgg atgctgcgga cgccgccgcc gcgaaactcc gtgcgctcgc
    10141 ggtcggggaa ggcgaggtag tcggtgaagc ggatgggcag ggtggcgacg ccgtccttgt
    10201 acggcgtgaa gtagccgctg gagacgaccg tccgcacgtc gaggaaggcg cgcccgccca
    10261 acgcctcgac ggcgcgcttg atgacggcct ccgcgcgctc gtccgccggc ggcggcgaag
    10321 ccttgacgga cggaggggca ggggactgag cggggctttg gccggcttga gaaagtgcga
    10381 cgcccgcggc ggcgaagacg aaaagcgcgg ccagacacgc gcgcgcgaag ggtggagaag
    10441 atttcttaat catggtgttt ttataaatcg gatgagtggg gagctgagac gaccggcgag
    10501 agagtataaa gcctgaaggg gcgttcggac aaaagttgga ggcaataaac cccggctttg
    10561 tccaaacgcc ccttcaagga ttcttcgtcg cccccgtctt caggagactt cggcggcgtc
    10621 ctcggccgcg tcgatagtga cctctccctc gcgcacgtcc acgacgaagc gcggagccgt
    10681 cttccacatc gccgtgatcg gaagtttcac cttctcgtcg atgtggcgct tgaggaagcg
    10741 cgcgccgtag gtcgtgctga agcccttctc ggtgagcagg ttgatggcct cctcggtgat
    10801 gtcgagcacc ttgccctgat gctccatctg cgcgcggagc cggcccagat agaggcgggc
    10861 gatctcgcgc acttcgtcca tcgtgagcgg cgagaagatg acgatctcgt cgatgcggtt
    10921 gcgaaactcc gccgagaagc gcgtctcggc ggccttcatc acgtcgccct tgatggcctt
    10981 catgtcgccc agcgttttcg tcccgaaccc gagcggcttc tcgtacttct tgaaattctc
    11041 cgagccgagg ttcgaggtca taatgacgat ggcgtcggag agataaacct tcttgccgcg
    11101 cccgtccgtg agccagccct cgtcgaacgc ctgaagaaag agattcatca gcgtcgggct
    11161 ggccttctcg acttcgtcga gcaggagcac cgtgtacggg ttctcacgca actgctccgt
    11221 cagaatcccg ccgcgctccg agccgacgat gccgcgcggc atcccgatca gtttctcgat
    11281 gccgatagtg ccgtccatgt attccgacat gtcgatgcgg accatcttct cgtcgtcgcc
    11341 gaacatcgac tcggcgacgg ccttcgccag ctcggtcttg ccgacacccg tcggcccgag
    11401 gaagaggagc acgccgtcgg gcttgtagtg cgactccttg agcggcccct tgttgagacg
    11461 caggcgctgc gcgacggagt ggacggcttc cttctgccca atgacgcggt gccccagctc
    11521 gtcctccatg ttgctgaagc ggtcggtcgt gtcgcggaag atcatgtcgc gcgggatgcg
    11581 cgactcctgc gagataacat cgatgatgtg ctcgggcctg accaccatcg cgtctggctc
    11641 gttgatctcg accttgacgg ccgccgtgtc cagccagccg atggccttgt cggggaggtg
    11701 caggttgcgg atgtacttcg gcgccatctc cagcgccgtg ttgatggcgt cgtcggagat
    11761 ggtgacggag tagttcttct ccagacgggg ccggaggccg aagaggattt cgcgcgtctc
    11821 ggagatggtc ggctcggcca ctttcacctg ccggaagcgg cgggcgaggg cctcgtcctc
    11881 ggcgatgtac tccttgaact ccgtgttggt cgtcgcgccg atgattcgca tctcgccgcg
    11941 cgccagcgcc gacttgaaca tgttggcggc gtcactggat gtgccgagcg ccgcgcccgc
    12001 gccgatgatg gtgtgggcct cgtcgataaa gaggatcagg tcttcactct ccttgacctc
    12061 gtcgatgatg cccttgatgc gctcctcgaa catgccgcgc agcatggtgc cggcgacgag
    12121 tccgcccatc tggagctgga cgatgtgcga attgcgaagc cgcgccggaa ccttctccgg
    12181 ctcaagctcg acgagacgcg ccaagccttc gaccactgcc gttttgccga cgcccggctc
    12241 gccgacgagc atgggtgagt tggcgcgttc gcggtggcag aggatttcga tcatctgctg
    12301 aatttccatc tcgcggccga tggtcggcgg aattttgtcg gcgcgggcga ggcggtttaa
    12361 agagacgccg aaatgcttga ggtaaggcgg cagctcgaac ttcttcttgt actgctcgtc
    12421 ctcctgctcg cgcgtccgca cccgtgcgcg cacctgctcg acgattgact ccgggctggc
    12481 cccgaggtta cgcaggacgc cgacgaagac gctgcgctcg tcctgcgaga gcgccatgaa
    12541 gaggtctgta gcctcgatgg tcttgcgccc ctgagcgcgc gcgcgctcca tggcgcgctt
    12601 gaagaggtcg atggtctcgg gcgcgatgcg gatgcctttg gcgacgttct ggtagcgcgc
    12661 ggcctccaga cgcttctcga tcaacacctt gaccgagcgc gggtctaagg agaggtcgcg
    12721 catcatcgag ttgaagagtt cgtcctcctc gttggcgagg gcgctgagga tgtgttcgac
    12781 ggcgatgtag ttctggtcgc ggttgcgtga ttcctgcacg gcgcgctcga agacgcgctg
    12841 gccgctttcc gcgaaccgat ctttgtaagt tccaaggtct accattttat ttcccttcca
    12901 tgatgaccgg agactgtacg tcgggagcgt cgcacctcgg ccctcacaaa accccttgat
    12961 agtttacgcg cggcgacgaa aatcggtttc cgtggtcgaa aacccgtgat ttcgccccgc
    13021 gcgtccacac cctttggatg caatgaactg tgtccgccgt tgtgccctgc ggcgagaagc
    13081 gcgttgcgct tcaggacact tcggatagta gcacaaaggc ggaagcaaat tttcggccat
    13141 gttatcaagg caaaagtaaa aaggtaaaag gcaaaagcgg aagagacact tcccacacac
    13201 ttttgccttt ttccttttac cttttgcctt ctttagtaat cgccgtagtc ttcgccgcct
    13261 cccatctgcg gggcggcggc cctcttcggc tcgggcaact cggtgacggc cgcgtcggtc
    13321 gtcaggagca gcccggcgac ggagacggcc atttgcaagg ccgtgcggac gaccttcgtc
    13381 gggtcgatga tgccggcgga agccaggtct tcgtacttgc ccgtggccgc gttgaagccg
    13441 gtcttgccct tcgactcctc gaccacgttg atggcgatgg agccgtcaac gcccgcgttc
    13501 tcggcgatgc ggcgcagcgg ctcctgcatc gcgcgcttga gcagacggat gcccgtcagc
    13561 tcgtcgccct ccgcgtcgag cttgtcgagc gcgcgcgcgg cgcgcaccaa cgccacgccg
    13621 ccgccgggga cgatgccctc ctgcgccgcc gcgcgtgtgg cgttgaccgc gtcgtcaacg
    13681 cgcatcttct tctctttcat ctcggtctcg gtggccgcgc caacgcggat caccgcgacg
    13741 ccgccgacga gcttggcgag gcgctcctgc aacttctcgc ggtcgtagtc cgaggtggtc
    13801 tcatccatct gccggcggat ggcctggacg cgtccctcga tggcgcgcgg ctcgcccgcg
    13861 ccctcgacga cggtcgtcga gtccttgtcg atgatcacgc gcttggccgt gcccaaatct
    13921 tcgagcgtga ccgattcgag cttgacgccg atgtcctcgg tgatgagacg cccgcccgtg
    13981 agcgtggcga ggtcttcgag gatggccttg cggcggtcgc cgaaggccgg ggccttgacg
    14041 gcgcagacct tgacggtgcc gcgcaggcgg ttgacgacga gcgtggcgag cgcgtcgccc
    14101 tccacgtcct cggcaatgat gaggagcgag cggcccttct ggagcacctg ttcgaggagc
    14161 ggcaggaggt cgcgcatggc ggcgaccttc ttctcgtgga tgaggatgag cgggtcgtcg
    14221 agcacgcact ccatgcggtc ggcgttcgtc acgaagtagg gcgacaagta gcctctgtcg
    14281 aactgcatgc cctcgaccag atcaagctcg gtctggagcg tgcgcgactc ttcgaccgtg
    14341 acgacgccgt cgcggccaac cttctccatc gcctcggcga tgaggtcgcc gatctcgcgg
    14401 tcgttgttgg cggagacggc ggccacgttg gcgaggtcag acttgtcctt caccgtcttc
    14461 gacatcttct ccaatgcttt aacggccgcc tcgacgcccc gttgaaggcc gcgctggagc
    14521 aacatcgggt tggcgccggc cgtgacgttc ttgacgccct cgcggaagat ggcctgcgcg
    14581 agcacggtgg ccgtcgtagt gccgtcgccc gcgatgtcgt tcgtcttcga cgcgacctcg
    14641 cgcaccatct gcgcgcccat gttctcgtag ttgtccttga gctgaatttc tttggcgacc
    14701 gtcacgccgt ccttggtaat aacgggcgag ccgaactt
//
LOCUS       DASVES010000350        12645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_634608, whole genome shotgun sequence.
ACCESSION   DASVES010000350 DASVES010000000
VERSION     DASVES010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634608"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..2851
                     /locus_tag="VF240_14310"
     CDS             386..2851
                     /locus_tag="VF240_14310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7176434.1"
                     /translation="MGEVYKAEDLKLNQTVALKFLPEDFARDRAALEKFFGEVRTARQ
                     VSHPNVCRVFDIGETGGSHFLSMEFIDGDDLSSLLRRIGRLPHDKAVEIARQLCAGLH
                     AIHQVGVLHRDLKPANAIIDGRGKARITDFGIAGLESELRDGAGLAGTPAYMSPEQIT
                     GTELTKKSDIYSLGLVLYEIFTGRQAFEGETINELIRKHKRAAPTTPSEFVKEIDPLV
                     EQTILRCLEKDPRERPASAMQVALMLPGGNPLEAAIAAGETPSPEMVAAAPKKGALKP
                     AVAVACLSAVVALFAFIVFFSGRVKSSEWIPLDKPPEVLAERARQIIDRLGYTDAPTD
                     TVYSFGSAGSYINYAGREDAPPDSFEKIRTGQPLLLYFWHRTSPRYLIPNAGTEVNFF
                     DPPHDVSGMTSVSLDVRGRLVEFQAVPPQVEEKETHNTPADWSTLFAEAGLDIRSYKE
                     IESQWTPPFFADERRAWEGRHVDHAEIPVRIEAAAFHGRPVYFTVISPWDKPARQTDW
                     RRAVGSTGIILAITFFSLLSAALIIARYNLKAGRGDTKGAFKLAVFVFSVITLARLID
                     AEHVPDLGGEIWLFFQGVSLALFISTVTWIFYIALEPFLRRRWSELLISWSRLMAGDF
                     RDPLVGRDVLVGGLMGLFSTAVNYLTILVPQWSGVATPPIMQGNPEAFSGVGGMAIHF
                     LHIFAERIYFTIFLLFLLLLLLTVLRKKWLAIAAMWLIIFLTDVDFAGGHQWIFWLHL
                     LLTATFITLAAARFGLLTLYSFSLFSSLSSAFPITSDFSNWYAGSTLFVFVVLMGLAI
                     YGFHTSLAGQKIFEAKFLTDVES"
     gene            3132..6134
                     /locus_tag="VF240_14315"
     CDS             3132..6134
                     /locus_tag="VF240_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7176435.1"
                     /translation="MLNRLIRLCACLVFIFGAAPKHHARQQQAAATLRGIVTDPNGAV
                     IAGALIRATQTSTGTARETQTNDEGAYVLTNLPPSEYEVRVEARGFASKVTKSPVVLQ
                     VGQNVTLDVPLEIDVRESMTVDLESTVQLIDTNASQVDQVIDEREIRNLPLNGRNFLE
                     LALLTPGNSPAPNFDPTKTNTVVISSAGQFGRGGSVTVDGADTNDDVVGGAVQNISQD
                     AVQEFQIATNRFDARLGRTGSAVINIVTKSGTNAFHGSAAAFFRDAKLQGLPATFDRA
                     SAQEEPPFDRQQYAFTLGGPIRRDRAWFFGSFEYRNQDGAVLVGARDPARRVITRGFA
                     DAPLDDLLSTNRVDLAPSDRDRLSFRYSVQDDESVGASTLVRSIGSASQRQSSHNRAH
                     SFLANYTRTLTAEAVNDFRFSYSTFINRTVPVAPGPQLTFPSIQDGASFRVPQETRQK
                     RFQFSDTLTMLRGNHTLSVGGEFQHVRSGFRLDVFQQGRVELIEDFPDFDRNGDGRTD
                     DDDLLFAVTLRSAFPDRPLLIPDANNNYFAAFVQDDWRLRPNLTLNLGLRYEIDSDVK
                     NVSRYDEINPIVRPFLRGTRSRDANNFAPRVGFNWATRDGRTSLRGGYGIYYDRVTLQ
                     IQSLERGLDGRALPIEVRAGNLFFIPPPCLFDPSCGQFPPPAPTLSSPFTGFLLPGAG
                     AGGINIIDNDLQNPLVQQMTIGIQHEFARDYVVRADYLHNFGTHFIIGRTIGQVFNPV
                     VGGPDLVKNLESSVKTKYDGLLLSVEKRFSRPVQFRASYTLSKAFNYANDDQIPFSNG
                     PANPENLQLEYGPTPNDQRHRFTFAGVFDAPLGLRLSPLLTLASGVPMDILLPDGSAR
                     LPSLQRNAGGRLFETGAELNAFITQLNAAGGVGAQPLPLVSDDARFSDGFSSLDLRVS
                     KDFRIGERVRLEPIAEVFNLFNVTNILGVSNVNYSGFSNVLVRDSNEASTPGFLRSSS
                     FGRPVTTAGGVFGSGGARAFQFAARLTF"
     gene            6286..7620
                     /locus_tag="VF240_14320"
     CDS             6286..7620
                     /locus_tag="VF240_14320"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7176436.1"
                     /translation="MSSTDKLKRQIGLRTATALVVGEVIAVGIFLAPAGMAKSLGSPA
                     LVFGVWLAMGLMALCGALCYGELAARFPEAGGGYVYLREAYGPAVAFLYGWMAFLVMD
                     PGLTAALAVGMAGYAAYSLGLSTLGTKAVAIVAIVAVAAVNIRGVRLGGWFVRWLTVV
                     KLGLLLLILLWGFALRLGDWGNFTPFVAQRAGSTPLAGALAGGLVAAFFSFGGWWDVS
                     KLAGEVRDPERTLPRALAYGVLTVTLVYILTSAVFIYLVPLDRVTSSETFAAQAGEAL
                     FGRLGGQFFSGVVIIAVLGSLVGIVMSAPRVYFAMARDRVFPAGAARLHPRYGTPARA
                     IALQAALASVLVVLGNFEQIVSYFIFVVVVFIALTVAALFVLRRERPAAGGYSTPGYP
                     VTPVIFLLLIVLLLLLLGGNNPKQAFLGVAVVAAGLPFYWLVFRRAPGGSES"
     gene            7640..7924
                     /locus_tag="VF240_14325"
     CDS             7640..7924
                     /locus_tag="VF240_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176437.1"
                     /translation="MTWIKTIPFDEADEKLRRAMEAQRGLYPAEYAEPVHPTPDGETS
                     GIVASHSLIPDALYHAFATFGVLTSPDLPLTRAQHEMITTVVSVANRCHY"
     gene            7961..8209
                     /locus_tag="VF240_14330"
     CDS             7961..8209
                     /locus_tag="VF240_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176438.1"
                     /translation="MDEELVAALQADYTTARISEQDRVMLDFVAQLTKDATRITPDDH
                     ARLRAHGFDDKGILQITLIASWFNYINRVADALGVGRS"
     gene            8425..9561
                     /locus_tag="VF240_14335"
     CDS             8425..9561
                     /locus_tag="VF240_14335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7176439.1"
                     /translation="MPTENLHALAALIRRERDTLLAEWRQEVRQLSVAHHLDVPTLND
                     HIPDLLEELADELEAHVDESMIGELKKNSVIHGLDRLRLGFDIEEVVGEYNALRGVLQ
                     DLIERHNLSLRGPVNRTINRVIDMSIGLAVKTYATQKALEIQQRREEHLAFVAHDLRS
                     PLSVISMAAQLLERTVPDVVKDERAAMLLETMHRNVGRLNSLVVKVVQEEANLKAKVN
                     ERVERRKVELRALVEVLISDIHPLAEAAHTRLLNEVPAELTAFADANMLTLIFQNLFS
                     NAITYTPGGEVIIGARESKEPDFLECWVSDNGAGIPADRLEKVFDKLETDPNKKSGMG
                     LGLAIVKQFVEAHGGQVTVDSKLGQGSTFRFTIPYQAEVVSEHS"
     gene            complement(9692..12211)
                     /locus_tag="VF240_14340"
     CDS             complement(9692..12211)
                     /locus_tag="VF240_14340"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX7176440.1"
                     /translation="MSEDDSSRDDLRLLIRRLESLEHVLRGHTQRLYTIERRLGIDFL
                     YSPPQQQHPPAEEIKTEPSAAAPPRLDGEPDAPFKTPTPTPNPTAASMPQAEPRASGQ
                     TQPYESPYGRRGAGADAGPAAGAGWARSGGGGAAGSVLFGSGEHATAQPKRARDMESL
                     VGGSWFSWLGIIAIVFGVAFFLKYAFDSEWVGPGARVALGAVAGLSIIVLAEVLRARG
                     LRQYAHVLSGGGLLILYLSVYAARAFYEMMGQPVAFLLMSLVTTGAVALSVRHDALAI
                     AILGLIGGFFTPVLLSTGQDNQVGLFTYVALLDAGVLAVAHFKRWRVLNFLSFFATAA
                     MFVGWMDTHYLPEKLGPSVFFLTLFFLLFSALAPVQNVLGRQGARWYDVLLVVLNATF
                     YYTLAYSLLDGAGYTPGPLGFFTLAVAALYALLYVLTRSRRPADRLLAYSYVGAAVTF
                     LTLALAVWLEQQWMTVGWAVEGLALVWVGLRGRERALRRVAMFVFVVALVRWFAADMS
                     DFGYSAMGAGFVPLWNARAFSAAVLVAALVAAAWAYRRAALDDEEERAGVSAFLTFSA
                     NAVALMLLTLDANDYFEARLARLAGESGDQWGRVENTRQLTLSALWTLYGAAALSIGI
                     GRARRGLRYAGLALLAVAALKVLLLDLRFYDAAWHAPLFNQTFAAFALLVAALCYALR
                     LYSRAEKFEETDRVVPALVVAANLLAVVALSAEASGYFAAGSGPDEPTAGAARDLRLA
                     RELSLSIVWALYGGALLAYGHLRRRRLPRLLALALLGLTTLKVFFWDLSSLDRIYRII
                     SFVVLGLILLAVSYLYQKSQRAAGEEEAEATGAASEGQPSG"
     gene            complement(12443..>12645)
                     /locus_tag="VF240_14345"
     CDS             complement(12443..>12645)
                     /locus_tag="VF240_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176441.1"
                     /translation="AAQAGGVAAQKAGAPKVVAHAGGEASGAAKNSDLITKVAETAGH
                     TAGRVARAVTNVIETLTGGKKR"
BASE COUNT         2307 a         4169 c         3884 g         2285 t
ORIGIN      
        1 ctccgcggct cggcggtgaa tcacggctga aggccactca ccaccgagtc gccgaggacg
       61 ccgaggtcgc gcagagaact gaaattagga cacgaccaaa attattggtg tcgtcgtccg
      121 acaagtcata taacataccg ctccgatgaa ccgttgcccc gcctgctcaa ccgccgtcgc
      181 tgaaaatgtt ctcgcctgcc caacctgcgg cgctgtgctg gcaggcacac agccgaccgt
      241 gcgccaccct ccgccggaag aaaacagcag accccggaaa agctctcaga cgagttccac
      301 aagctcgcgc aacgaaggtc atttcatcgc gggaacgata ctgaacgagc ggtatcgcgt
      361 catcggcctg ttaggcagag ggggaatggg cgaggtctat aaagccgaag acctcaagct
      421 taatcaaacg gtcgcgctga agttcctgcc ggaagatttc gcccgagacc gcgcggcgct
      481 cgaaaagttc ttcggcgaag tccgcacggc gcgtcaggtg tcgcacccaa acgtctgtcg
      541 cgtcttcgac atcggggaga cgggcggctc tcacttcctc tcgatggagt ttatcgacgg
      601 cgacgacctg tcgtcgctgc tgcggcgcat cggccggctt cctcacgaca aggcggtgga
      661 aatcgccaga caactctgcg ccggacttca cgccattcat caggtgggag ttttgcaccg
      721 cgacctcaaa cccgccaacg ccatcatcga cgggcgcggc aaggcgagga ttacggactt
      781 cggcatcgcg ggtctggaga gcgagttgag agacggcgcg gggctggccg gcacgcccgc
      841 ctacatgtcg cccgaacaaa ttacgggcac agagttgacg aagaagagcg acatctactc
      901 gctcggcctc gtgctctatg aaatcttcac aggcaggcaa gcctttgaag gcgagacgat
      961 caatgagctg attcgcaaac acaaacgggc cgcgccgacc accccttcgg agttcgttaa
     1021 agagatcgac ccgctcgtcg agcagacaat tcttcggtgt ctggaaaaag acccgcgcga
     1081 gcgtcccgct tcggcgatgc aggtggcgtt gatgctgccg ggcgggaacc ctctggaggc
     1141 ggccatcgcc gccggggaaa cgccctcgcc cgagatggtt gccgccgcgc cgaagaaggg
     1201 cgcgctcaaa ccagccgtcg ccgtcgcctg cctgtcggcg gtcgtcgcgc tattcgcgtt
     1261 catcgttttc ttttcgggca gagtaaagag ttccgaatgg attccgctcg acaagccgcc
     1321 cgaagtttta gcggaacgcg cccgccaaat catcgacagg ctcggctaca ccgacgcgcc
     1381 gaccgacacg gtctactcgt tcggctctgc cggaagttat attaattacg ccggacggga
     1441 agacgctccg cccgacagtt ttgaaaaaat ccgcacggga caacccctgt tgctctattt
     1501 ctggcatcga accagcccgc gttacctcat cccgaatgcg ggtactgaag taaatttttt
     1561 cgaccctccg cacgacgttt ccggaatgac gagcgtctcg ctggacgtgc gcggccgcct
     1621 cgtcgaattt caagccgtcc cgccgcaagt cgaagagaaa gagacgcaca acaccccggc
     1681 cgattggtca acgcttttcg cggaggcggg cctcgacata cgcagttaca aagagatcga
     1741 atcacaatgg acgccgccct tctttgccga cgagagaagg gcctgggaag gcagacacgt
     1801 tgaccacgcg gagattcccg tccgcatcga ggcggccgcc tttcacggaa ggccggttta
     1861 tttcaccgtc atctccccct gggacaagcc tgcgagacaa acagattgga ggagagcagt
     1921 cggcagcacg ggcattattt tagccattac attttttagc cttctgtcag ccgccctgat
     1981 aatcgcccgt tacaatttga aagccgggcg cggcgatacc aaaggcgcgt tcaaactcgc
     2041 cgtcttcgtc ttttcggtga tcaccctcgc ccgactcatc gatgccgaac acgtccccga
     2101 cctcggcgga gaaatctggc ttttttttca gggcgtcagt ctcgcgcttt tcatctccac
     2161 cgtgacgtgg attttttaca tcgcgctcga accgtttctc cgccggcgct ggtcggagtt
     2221 gctaatctcc tggagccgtc tgatggcggg agatttccgc gacccgttgg tcgggcgcga
     2281 cgttctggtc ggtgggctga tggggctttt cagcacggcc gttaattacc tgacgattct
     2341 cgtgccgcaa tggtcgggcg ttgccacccc gccgattatg caggggaacc ccgaagcgtt
     2401 ttcgggagtc ggcggaatgg cgatccattt tttacacatc tttgccgagc ggatttactt
     2461 tacgattttt cttttatttc tgcttttact tctgcttacc gttttacgaa aaaaatggct
     2521 ggcgatagcg gcgatgtggc taattatttt tctcaccgac gtagattttg cgggcggtca
     2581 tcaatggatt ttctggcttc acctgctttt gacggcgacc ttcatcacgc tggccgcagc
     2641 gcgtttcggt ctgctcaccc tttactcctt ttctctcttt tcctccctga gttccgcttt
     2701 tcccatcacc tccgattttt ccaactggta tgccgggagc actttattcg tcttcgtcgt
     2761 gctgatgggc ttggcgattt acggctttca cacatcgctc gccggacaaa agatttttga
     2821 agcgaaattc ctgacagatg tcgagagtta aaattgaagc tgttataaat agatgttagg
     2881 cggctggtgc tgaacggaac ttgataaccg tattttcctg ctcgccatca ttgactagac
     2941 gaaacatcgg cggtgcatta aattcgacgt gtcggtggtc atttattgaa tgcgccttcg
     3001 cgatattaaa gcccacgccg tgtctgagac ccaacccgca ggatgagggg cgcacgactt
     3061 cgagcgtcgt tgtcgtgccc cgatgccggt acgaaacatc gtccttccga acatttctta
     3121 gaggaagatt tatgctcaat cgactcatca ggctgtgcgc ctgtctcgtg ttcatcttcg
     3181 gcgccgcccc caagcatcac gcccggcaac agcaagcggc ggcgacgctc aggggcatcg
     3241 tcacagaccc gaacggcgcg gtcatcgccg gcgcgctcat cagggcgacg caaacgtcca
     3301 cgggcacggc ccgcgagaca cagacgaacg acgaaggcgc ttacgtcctg acgaacctgc
     3361 cgccgagcga gtacgaggtg cgggtggagg cgcgtgggtt cgcttccaaa gtcactaagt
     3421 cgcccgtcgt cctgcaagtc gggcagaacg tcaccctcga tgtgccgttg gaaattgacg
     3481 tgcgcgagtc gatgaccgta gaccttgaaa gcacggtgca gctcatcgac acgaacgcct
     3541 cgcaggtgga tcaggtgatc gacgagcgcg agatcagaaa cctgccgctc aacgggcgca
     3601 acttcctcga actcgcgctg ctcacgcccg gcaactcgcc cgcccccaat ttcgacccga
     3661 ccaagaccaa cacggtcgtc atctcctcgg ccgggcagtt cggccgcggc gggagcgtca
     3721 ccgttgacgg cgcggacacg aacgacgacg tggtcggcgg cgccgtgcag aacatctcgc
     3781 aggacgcggt gcaggaattc cagatcgcca ccaaccgctt cgacgcgcgg ctcggccgca
     3841 ccggctccgc cgtcatcaac atcgtcacca agtcgggaac gaacgctttc cacggctcgg
     3901 ccgccgcctt cttccgcgac gcaaagttgc agggcctccc cgccacgttc gaccgcgcct
     3961 cggcgcagga ggagccgccc ttcgaccgac agcagtatgc gttcaccctc ggcggcccca
     4021 tccggcgcga ccgggcgtgg ttcttcggct ccttcgagta tcgcaatcag gacggcgcgg
     4081 tgctcgtcgg cgcgcgcgac ccggcccgcc gcgtcatcac gcgcggcttc gccgacgcgc
     4141 cgctcgacga tctcctctcg accaaccgtg tagaccttgc gccgagcgac cgcgaccggc
     4201 tcagcttccg ctactcggtt caggacgacg agagcgtcgg ggcgagcacg ctcgtccgct
     4261 ccatcggctc ggcctcgcag cgacagtcga gccacaaccg cgcgcactcc tttctcgcca
     4321 actacacgcg cacgctcacc gccgaggccg tcaacgattt ccggttcagc tacagcacct
     4381 tcatcaaccg caccgtgccc gtcgcgccgg ggccgcagct caccttcccg agcattcagg
     4441 acggcgcctc gttccgcgtc ccgcaggaga cgcgccagaa acgtttccag ttctccgaca
     4501 cgctgacgat gcttcgcggc aaccacacct taagcgtcgg cggcgagttc cagcacgtcc
     4561 gctccggctt ccggctcgac gtgttccagc agggccgcgt cgaactcatc gaagacttcc
     4621 ccgacttcga ccgcaacggc gacgggcgca ccgacgacga cgacctgctc ttcgccgtca
     4681 cgctccgcag cgccttcccc gaccggccgc tcctcatccc cgacgccaac aacaactact
     4741 tcgccgcctt cgttcaagac gactggcggc tgcgccccaa cctgaccctc aacctcggcc
     4801 tgcgttacga aatcgactcc gacgtgaaga acgtcagccg ctacgacgag atcaacccca
     4861 tcgtccgccc cttcctgcgg ggcacgcgct cgcgcgacgc gaacaacttc gccccgcgcg
     4921 tcggcttcaa ctgggcgact cgagacgggc gcacctccct gcgcggcggc tacggcatct
     4981 actacgaccg cgtcactctc cagattcaat cgctggagcg gggcctcgac ggccgcgcgc
     5041 ttcccatcga ggtgcgcgcc ggcaacctct tctttatccc gccgccgtgt ctcttcgacc
     5101 cctcctgcgg gcagttcccc ccgcccgcgc ccacgctctc ctctcccttc accggcttcc
     5161 tcctgcccgg cgccggggcg ggcggcatca acatcatcga caacgacctc cagaacccgt
     5221 tggtgcagca gatgaccatc ggcatccagc acgagttcgc gcgcgactac gtcgtgcgcg
     5281 ccgactacct gcacaacttc ggcacacact tcatcatcgg ccgcaccatc ggccaagtct
     5341 tcaatcctgt ggtcggcggc cccgacctcg tcaagaatct tgagtcgagc gtcaagacga
     5401 agtacgacgg cctgctcctg agcgtcgaga agaggttttc gcggccggtt cagttccgcg
     5461 cctcctacac gctctcgaag gcgttcaact acgcgaacga cgatcagatt cccttctcga
     5521 acggccccgc caacccggaa aacttacagc tcgaatacgg ccccacgccc aacgaccagc
     5581 gccaccgctt caccttcgcc ggcgtcttcg acgcgccgct cggcctgcgt ctctccccgc
     5641 tgctcacgct cgcctccggc gtgccgatgg acatcctcct ccccgacggg agcgcgcgcc
     5701 tcccctcctt gcagcgcaac gcgggcggcc gactcttcga gacgggcgcg gagctgaacg
     5761 ccttcatcac gcaactcaac gcggcgggcg gcgtcggcgc tcaacccctg ccgctcgtct
     5821 ccgacgacgc gcgcttcagc gacggcttca gctcgctcga cctgcgcgtc tcgaaggact
     5881 tccgtatcgg cgagcgcgtg cgcctcgaac ccatcgccga ggtcttcaac ctcttcaacg
     5941 tgacgaacat cctcggcgtc tcgaacgtca actactcggg cttctcaaac gtgctcgtca
     6001 gagacagcaa cgaagcgtca actccgggct tcctgcgctc gtcgagcttc gggcggcccg
     6061 tcacgaccgc cggcggcgtc ttcggctcag gcggagcgcg cgccttccag ttcgcggcgc
     6121 gtctgacgtt ctgaagaagc tgtgagctat tagccgttag ctgttagctc acggccgtgc
     6181 gccgtggcgc gcgtcccaca ggggcactaa agtcctcgcc cgctctcttc taaagctaat
     6241 agctaacagc taatggctaa tggctaatgg ctaacgccta atcccgtgag ttcaacggac
     6301 aagctcaagc gacagattgg cctgcggacg gccacggcgc tggtcgtcgg cgaggtgatc
     6361 gccgtcggca tctttctggc gcccgccggg atggcgaagt cgctcggctc gcccgcgctc
     6421 gtcttcggcg tctggctcgc gatgggtttg atggcgctct gcggcgcgct ctgttacggg
     6481 gagttggcgg cgcgtttccc agaggccggg ggcggctacg tctatctgcg cgaggcttac
     6541 gggccggcgg tcgccttcct ctacgggtgg atggccttcc tcgtgatgga cccggggttg
     6601 acggccgcgc tcgccgtcgg catggccggc tacgcggcct actcgctcgg cctctcgacg
     6661 ttggggacga aagccgtcgc catcgtcgcc atcgttgcgg tggccgccgt caacatccgc
     6721 ggggtcaggc tcggcggctg gttcgtccgc tggctgaccg tcgtcaagct cgggctgctc
     6781 ctgctcatcc tgctgtgggg cttcgcgctg cgtctgggcg actggggaaa cttcaccccg
     6841 ttcgtcgccc agagagcggg ttcgactcca ctcgcggggg ctttggccgg gggactggtg
     6901 gccgccttct tctccttcgg cggctggtgg gacgtgagca aactggcggg cgaagtacgc
     6961 gaccccgagc gcacgcttcc ccgcgcgctg gcctacggcg tgctcacggt gacgctcgtt
     7021 tacatcttaa ccagcgccgt cttcatctac ctcgtgcccc tcgaccgcgt cacttcgagc
     7081 gagacgttcg cggcgcaggc gggcgaggcg ctcttcgggc gcttgggcgg gcagttcttc
     7141 tccggcgtcg tcatcatcgc cgtgctcggc agtctcgtgg ggatcgtcat gtcggccccg
     7201 cgcgtctact tcgcgatggc gcgcgaccgc gtgtttccgg cgggggcggc ccgtctgcac
     7261 ccgcgctacg gcaccccggc gcgggccatc gccctgcaag cggcgctcgc ctctgtgctc
     7321 gtcgtcctcg gcaattttga acagatcgtc tcgtacttta ttttcgtcgt cgtcgtcttc
     7381 atcgcgctga ccgtggcggc gctcttcgtg ctccgccgcg aacgcccggc cgcgggcggc
     7441 tactcgactc ccggctaccc cgtgacgccg gtgatatttt tgcttttgat cgtcctgctg
     7501 ctcttgctgc tcggtggtaa caacccgaag caggcgtttc tgggcgtcgc ggtggtggcg
     7561 gcggggctgc cgttttactg gctcgttttt cgccgtgcgc ccggcggctc tgaaagttga
     7621 aagagaggaa tcacaaccca tgacgtggat caagaccatc cctttcgatg aggccgacga
     7681 aaaactgcgc cgggcgatgg aggcgcagcg cggactctac cccgcggagt atgccgagcc
     7741 ggtgcatccg acccccgacg gcgagacatc gggcatcgtg gcctcgcaca gcctcatccc
     7801 cgacgccctc taccacgcct tcgccacctt cggcgtactg acctcacccg acttgccgct
     7861 cacgcgggcg caacacgaga tgatcaccac cgtggtctcg gtcgccaacc gctgccacta
     7921 ttgaatcgag tcgcacgcag agtttctgcg tcgagtcacg ttggatgaag aactggtcgc
     7981 ggccttgcag gcggactaca cgactgcgcg catctcggaa caagaccggg tcatgttgga
     8041 cttcgtcgcc caactaacta aggacgccac gcgcatcacg cccgacgacc acgcgcgtct
     8101 gcgcgcccac ggcttcgacg acaaaggcat ccttcaaatc accctcatcg cttcgtggtt
     8161 caactacatc aaccgggtcg ccgacgcgct cggcgtcggc agaagttaaa tcaaacgcgc
     8221 gccggtgtcg cctcagatgt acatccttgt attacctcgt ataatgaatt tccccagcta
     8281 gtgagaattc cggccggagc gccgtcgccg ccgcgcccct cttgtagtga atcgaggagt
     8341 gagggtcgcc gcggagctaa acctttgaac gggacctcga cagtgtccag ctccactatg
     8401 acctgacgct tgacggagta aaaattgccc actgaaaatc tccacgcgct ggccgcgctg
     8461 atccggcgag agcgcgacac tttactcgcc gaatggcggc aggaggtgcg gcaactttcc
     8521 gtggctcatc acctcgacgt gccgacctta aacgatcaca tccccgattt acttgaggag
     8581 ctggccgacg agctggaagc gcacgtcgat gagtcgatga tcggggagtt aaaaaagaac
     8641 tccgtcattc acgggctgga tcggctccgc ctcgggttcg acatcgagga ggtcgtgggg
     8701 gagtacaacg ccctgcgcgg cgtcctgcaa gaccttatcg aaaggcacaa cctgagtctt
     8761 cgcgggccgg tgaatcgaac cattaaccgc gtcatcgaca tgtcaatcgg actcgcggtg
     8821 aagacctacg ccacgcaaaa agccctggag atacagcagc ggcgtgagga gcacctggcc
     8881 ttcgtcgccc acgacctgag gtctccactg tcagtcatct cgatggccgc tcagcttctc
     8941 gaacgtactg tcccggacgt ggtcaaggat gagcgggccg cgatgttgct cgagaccatg
     9001 caccgcaacg tcggccgctt gaattcactt gtcgtcaaag tagttcagga agaagcgaat
     9061 ctcaaggcga aggtcaatga aagagtggag cggcggaaag tagagctgcg ggcgttggtt
     9121 gaagtgctca tcagcgacat ccatccgttg gccgaggctg cacacacgag attgctcaac
     9181 gaagtgcccg cggaactgac agcgttcgct gacgccaaca tgttgaccct tatctttcag
     9241 aacttattct ctaacgcgat tacttacact cccggcgggg aagtcatcat cggggcgaga
     9301 gagagcaaag aaccggattt cttagagtgt tgggtgagcg acaacggcgc ggggattccg
     9361 gcagaccgtc tggaaaaggt cttcgacaaa ttagagaccg accccaacaa gaaaagcggc
     9421 atgggtctcg gcctggccat cgtcaagcaa ttcgttgaag cgcacggcgg tcaggtgacg
     9481 gttgacagta aactcggtca aggctcgacg tttcgcttca ccatacctta ccaagcagag
     9541 gtagtgtcag aacactcata aagccccacc catcaagccg gggagccgcc cttcgcgcaa
     9601 acgcatccgc gcgcattcac cacacgaccg ttccgccaga cgctcgaacg ccacgtcgca
     9661 gacacccgcg gtcatccgag tccacgcgga ctcaaccgga cggctgccct tctgacgccg
     9721 cgcccgtcgc ctccgcttcc tcttcgcccg ccgcgcgctg gctcttctgg tagaggtagg
     9781 agacggccag caggatgagg ccgaggacga cgaaggagat gatgcggtag atgcggtcga
     9841 gcgaggagag gtcccagaag aagaccttga gggtggtgag gccgaggagc gccagcgcga
     9901 gcaggcgcgg cagacgccgc cggcgcagat gtccgtaggc gagcagcgcg ccgccgtaga
     9961 gcgcccagac gatggagagt gagagttcgc gcgcgagacg caggtcgcgc gcggcgccgg
    10021 cggtaggctc gtccggcccc gacccggccg cgaagtagcc gctcgcctcc gcgctcagcg
    10081 cgacgacggc cagcaggttg gccgcgacga cgagcgccgg cacgacccgg tcggtttcct
    10141 caaacttctc ggcgcgcgag tagagacgca gcgcgtagca gagcgcggcg acgaggaggg
    10201 cgaaggcggc gaacgtctgg ttgaagagag gcgcgtgcca ggcggcgtcg tagaagcgca
    10261 aatcgagcaa gaggactttg agggcggcga cggcgagcag cgccagtccg gcgtagcgca
    10321 gaccccgacg ggcgcggccg atgccgatgg agagcgcggc ggccccgtag agcgtccaca
    10381 gcgccgagag cgtcagttgc cgcgtgttct cgacgcggcc ccattggtcg ccgctctcgc
    10441 ccgccagcct tgcgagacgc gcctcgaagt agtcgttcgc atcgagcgtg agcagcatga
    10501 gggcgacggc gttggcggag aaggtgagga aggccgagac gcccgcgcgc tcctcctcgt
    10561 cgtcgagggc cgcgcgccgg taggcccacg cggccgcgac gagcgcggcg acgagcacgg
    10621 ccgccgagaa ggcgcgcgcg ttccatagtg ggacaaagcc cgcgcccatc gcgctgtagc
    10681 cgaagtcgct catgtcggcc gcgaaccagc ggacgagcgc gacgacgaag acgaacatgg
    10741 ccacgcgccg gagcgcgcgc tcgcgaccgc gcaaccctac ccacacgagc gccaatcctt
    10801 cgaccgccca cccgacggtc atccactgct gttcgagcca cacggccagc gccagcgtca
    10861 ggaacgtgac ggccgcgccg acgtagctgt aggcgaggag acggtctgcg gggcgccggc
    10921 tccgcgtcag cacgtagagc agcgcgtaga gggccgcgac ggcgagcgtg aagaagccga
    10981 gagggccggg cgtgtagccg gcgccgtcaa ggagcgagta ggcgagcgtg tagtagaagg
    11041 tcgcgttgag gacgacgagc aacacgtcgt accagcgcgc gccctgccgg ccgagaacgt
    11101 tttggacggg ggcgagggcc gagaagagca ggaagaagag cgtcaggaag aagacgctcg
    11161 gcccgagttt ctccggcaag tagtgcgtgt ccatccagcc gacgaacatc gcggccgtgg
    11221 cgaagaagga gaggaagttg aggacgcgcc agcgcttgaa gtgggcgacg gcgaggacgc
    11281 ccgcgtcgag cagggcgacg taggtgaaga gcccgacctg attgtcctgt ccggtcgaga
    11341 gcagcacggg cgtgaagaaa ccgccgatga gtccgaggat ggctatggcc agcgcgtcgt
    11401 gtcggacgga gagcgcgacg gcgcccgttg tcacgagcga catcaggagg aaggcgacgg
    11461 gctgccccat catctcgtag aaggcgcgcg cggcgtagac cgagaggtag agaatgagca
    11521 ggccgccgcc cgagagcacg tgcgcgtact gcctgaggcc ccgcgcgcgc aggacttcgg
    11581 cgaggacgat aatggagagt ccggccacgg ctccgagcgc gacgcgcgct ccggggccga
    11641 cccactcgct gtcgaaggcg tacttgagga agaaggcgac gccgaagacg atggcgatga
    11701 tgccgagcca actgaaccaa ctcccgccga cgagcgactc catgtcgcgc gcccgcttcg
    11761 gctgagccgt cgcgtgctcc ccactgccga acagcacact gcccgccgcg ccgccgcctc
    11821 cacttcgcgc ccatcccgcg cccgcagccg gcccggcgtc cgcgcccgcg ccgcgccgcc
    11881 cgtagggcga ttcgtagggt tgcgtctgac ccgacgcgcg cggctccgct tgcggcatcg
    11941 acgcggctgt cggattcggc gtgggcgtag gcgtcttgaa cggcgcgtcc ggttcgccgt
    12001 cgagtcgcgg cggggcggcg gctgacggtt cagttttgat ttcttcggcc ggcgggtgtt
    12061 gttgttgcgg cggggagtag aggaagtcga tgccgagtcg gcgctctatg gtgtagagac
    12121 gctgcgtgtg ccccctgagc acgtgctcaa gtgattcgag ccggcggatg agcaggcgga
    12181 ggtcgtcgcg cgaggagtcg tcttcggaca tagggtttcc ttccgacgaa agagagggtt
    12241 cttccggcgc cgggcgctcc ctcctcgcga catgatagtt caattcgctt caaagttgga
    12301 ttgagtcgag cgagtttgtc tgcgggctgg aaccgggggg acgaaggcgc ataaaaaaga
    12361 agtcagacgc cgggtgtcgg atgtcagtca agacggcgcg tcttttaact gacatccggc
    12421 gcccggcgtc tgacctctgc ggttaacgtt tcttgccgcc ggtcagggtc tcgatgacgt
    12481 tcgtgacggc cctcgcgacc cggcccgccg tgtgtccggc ggtctcggcg actttcgtga
    12541 tcaggtcaga gtttttggct gcgccgctag cctccccccc ggcgtgagcc accacttttg
    12601 gagcccccgc cttttgagcc gccacgcccc ccgcctgtgc cgcgc
//
LOCUS       DASVES010000351         7043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1335697, whole genome shotgun sequence.
ACCESSION   DASVES010000351 DASVES010000000
VERSION     DASVES010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335697"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VF240_14350"
     CDS             complement(<1..571)
                     /locus_tag="VF240_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7176442.1"
                     /translation="MSAEVKERPPENLQRGLGLVDATMIVVGSMIGSGIFITSAESTR
                     LVGAPGWLLVAWALAGVLTITGALCCAELAAMMPKAGGQYVFLREAYGPGLGFLFGWS
                     LFLVIQTGTIAAVAVAFARFLGVLTPGVANNRYLVEPVHLGATGYAVSLSTEQLVAVL
                     LILVLTLTNTSGLRTGKFIQNTFTFTKTAA"
     gene            complement(695..2341)
                     /locus_tag="VF240_14355"
     CDS             complement(695..2341)
                     /locus_tag="VF240_14355"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176443.1"
                     /translation="MKRCPECRRTYADESLNFCRVDGVPLVADAAAADESEPTRFMPA
                     PTAASTGELPTEVFVRGGASGGVGTSASLTTPLPPGRRRRARAIDSLAVLPLENATGE
                     PQAEYLSDGITEAIINALSQLPRLRVVPRATVFRYKGSGADPQEVGRQLNVRAVVTGR
                     MHLLGDLLVVSAELVDVEQHAQVWGEQYRHKHEDIFTLQERISQEITTKLRLRLSGED
                     RKRLRKRPTENTEAYHLYLKGRYYLSKRTTDWIRKGVEHFQRAIDIDPAYALAHAGLA
                     DAYAFLASSTGEQPPAEFYPKAEAAARRALEIDDTLAEAHSSLGFYHLLYEYDFERAG
                     RAFARAVELNPNYANAHDGQSFYFKAIDRHAEAVRACRRAQQLDPLSLFANVSLGWAY
                     HFARQYETALEHARKALELDPQFIFAHWLAGMAHAQRGALDEAAAALALAVEHSGGGL
                     TFKAHLGYVSALAGRRDEARGLLGELEGHAQVRYVPAYYFALIHLGLAKRAAAIEGFE
                     RAYEERSGFLAFIHVEPTLDPLRDEPRFKELARRIGPASQ"
     gene            complement(2352..3125)
                     /locus_tag="VF240_14360"
     CDS             complement(2352..3125)
                     /locus_tag="VF240_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176444.1"
                     /translation="MSDETGRPDAAPAPEGASRKLLATHAVLVGLTPLIPVPMVDDLV
                     KSHLLRRMVRELAAGRGWQLSHEEVEALTTERGGDGCLGGCLGQALLYPFKKVFRKIF
                     FFLEWKRAADLTSRTYHHGYLIDHALAPRADGARLIERKTAAEVGAAIDAVCREAPVK
                     PLESAVGATFRGSKNVLVGAAGILERALRRRGAPRPDPRAVAEAVESVEQEEERQIEG
                     VTRGLQGAVAEIPDEHFRALRYALDARLGVVTPERRERL"
     gene            3354..4691
                     /locus_tag="VF240_14365"
     CDS             3354..4691
                     /locus_tag="VF240_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7176445.1"
                     /translation="MIKDQFKELIETKRARVGVVGLGYVGLPLVVEFAQQGFHGVGFE
                     VDAKKADTINAGESYIGDVSSSAVKEMVEAGRLLATTDFDRLAECDAIIICVPTPLRK
                     TKEPDISYILAASGEIQKRLRRGQLVVLESTTYPGTTDEVLLPMFTETGLGLDKDFLL
                     AFSPERVDPGNPQFKTHNIPKVVGGCTDDSTEAAAMLYGQIVASVHPVTSARVAEAAK
                     LLENTFRAVNIGMANEMARLCYTLGIDTWEVIRAAATKPFGFMPFYPGPGLGGHCIPL
                     DPHYLSWKARQHGFESRFIGLAEEVNSRMPDHVVRLISDGLNDERKSLNGSRVLILGV
                     AYKKDIDDVRESPALSIIDRLRAKGADVRYHDPYVAELRFDDAHTEGSGEPIQSVLLT
                     DDELRASDCAVIVADHTGVDYERVCALSKLIIDTRNALGGELRRKSCAKIIRL"
     gene            complement(4774..5718)
                     /locus_tag="VF240_14370"
     CDS             complement(4774..5718)
                     /locus_tag="VF240_14370"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7176446.1"
                     /translation="MRILITGSRGFVGGGVGRFAARAGHEVLGVGRATQAAPAWPGAY
                     AQADVISSDLAPLVRDFAPDAVIHAAGTASVGASFVSPLDDLRAALLSFANTLDAVRR
                     SGRAPVVLFPSSAAVYGRPAELPAREDAPLNPISPYGFHKAACELLAREYAECFGARV
                     TVCRLFSVFGEGQRRLLVWELYKQLAGPAREVWLEGTGAETRDYLHIDDAADALLRLI
                     EREVAEAEPDAGRLRVVNVASGEETNVLELAERVRALVAPGKEVRCRALERPGDPQRW
                     RADTTRLRALAPGWQPRPFAQRLAQCVAAWRAGVVEHG"
     gene            complement(5861..>7043)
                     /locus_tag="VF240_14375"
     CDS             complement(5861..>7043)
                     /locus_tag="VF240_14375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7176447.1"
                     /translation="RAAPPAERAAERRRTPFVFVFDERTPTPDRDAGSARMFHILRSL
                     ARWSRPVFIPFNRPTGTNYESLLWREGIETASLVDYARLLRERRPYAAIFSRPEVADA
                     LLEDVRRASPGIKIVYDMVDAYFIRIGREAELSGDARARAEAARYREIETRLAQASDL
                     FWCASPEDRRALEREVSGRPVEIIPTIHTPHGRGLPFDERRDLLFVGNLYHRPNSDGV
                     QHFMREIFPLVREALPGVRLTIVGDNSSPEISAYASDAVRVTGYVPDVDPFFERSRVF
                     VAPIRFGAGVKGKIGESLAYGLPVVTTPVGAEGMGLEDGTSAMITPDARAFADAVVRL
                     YLDGELWQKLSDNGYAHVREHFSPEVVERRINEPLMRLGRAGSLPGQNTQQEQAAKPI
                     L"
BASE COUNT         1143 a         2307 c         2458 g         1135 t
ORIGIN      
        1 gcgccgccgt cttcgtaaac gtgaaagtgt tctgtatgaa cttgcccgtc cgaagcccgg
       61 aggtgttcgt cagtgtgagc acgaggatga ggaggacggc gacgagttgt tcggtcgaga
      121 ggctgacggc gtagccggtc gcgccgagat ggacaggctc gacgagatag cggttgttgg
      181 ccacgcccgg cgtgagcacg ccgaggaagc gcgcgaaggc gacggcgacg gccgcaatcg
      241 tgcccgtctg aatgacgagg aagagcgacc agccgaagag gaacccaagc ccgggcccat
      301 aagcctcgcg caggaagacg tactgcccgc ccgccttcgg catcatcgcc gccagctccg
      361 cgcagcacag cgcgcccgtg atggtgagca cgcccgcgag cgcccacgcc acgagcaacc
      421 agccgggcgc gccgacgaga cgggtcgatt ccgccgacgt gatgaagatg ccggagccga
      481 tcatcgagcc gacgacgatc atcgtcgcgt ctacgaggcc gaggccgcgt tgcaagttct
      541 ccggcggacg ttcttttact tccgctgaca tctgtttggt ctgttgctta aaaggttgaa
      601 ccggcgaggc agcgcgtatg ttgtttcttc ggcactccgc tgtcaagaac ttttgagcga
      661 ggccgcgcaa gacttcagcg tccgccggtg ttgtttactg agacgccggg ccgatgcgcc
      721 gcgcgagttc tttaaaacgc ggctcgtcgc gcagggggtc gagcgtgggt tcgacgtgga
      781 tgaaggcgag gaagccggag cgctcttcgt aggcgcgttc aaatccttcg atggcggcgg
      841 cgcgcttggc caaaccgagg tggatgaggg cgaagtagta ggccgggaca tagcgcacct
      901 gcgcgtggcc ctcaagctcg ccgagcaggc cgcgcgcctc gtcgcggcgg ccggcgaggg
      961 ctgagacgta accgagatgc gccttgaagg tcagcccgcc gccggagtgt tcgacggcga
     1021 gcgcgagcgc ggctgctgcc tcgtcgagcg cgccccgttg cgcgtgggcc atcccggcga
     1081 gccagtgcgc gaagatgaac tgcgggtcga gttcgagcgc cttgcgggcg tgctcgagcg
     1141 cggtctcgta ctggcgcgcg aagtggtagg cccagccgag gctgacgttg gcgaagagcg
     1201 agagcgggtc taactgctgc gcgcggcggc aggcgcgcac cgcctcggcg tgccggtcga
     1261 tggccttgaa gtagaagctc tggccgtcgt gcgcgttggc gtagttcggg ttcaactcga
     1321 cggcgcgcgc aaacgcccgg cccgccctct cgaagtcata ctcgtagagc aggtggtaga
     1381 agccgagcga ggaatgagcc tcggcgagcg tgtcgtcgat ctccagcgcc ctgcgcgccg
     1441 ccgcctcggc cttcgggtag aactcggcgg gcggttgctc gccggtcgag gaggcgagga
     1501 aggcgtaggc gtcggcgagg cccgcgtggg cgagggcgta ggcggggtcg atgtcgatgg
     1561 cgcgttggaa gtgttcgacg cccttgcgta tccagtcggt cgtgcgcttg ctcaggtagt
     1621 agcggccctt gaggtagagg tgataggcct cggtgttttc ggtcggccgc ttgcgcagac
     1681 gcttgcggtc ttcgccgctc agacgcagcc gcagcttcgt agtgatctcc tgcgagattc
     1741 tctcctgaag cgtgaagatg tcttcgtgct tatggcgata ctgctcgccc cagacctgcg
     1801 cgtgctgttc gacatcgacg agttccgcgc tcacgacgag caggtcgccg agcaggtgca
     1861 tgcgtcccgt gactaccgcc cgcacgttca actgacgccc gacctcctgc ggatcggccc
     1921 cgctcccctt gtagcggaag acggtggcgc gcggcacgac gcgcaggcgc ggcaactgcg
     1981 agagggcgtt gatgatggcc tcggtgatgc cgtcggagag atactcggcc tgcggttcgc
     2041 ccgtcgcgtt ctccagcggg agcacggcga gcgagtcgat ggcgcgcgcg cgccggcgcc
     2101 ttccgggcgg cagcggcgtc gtcagagagg cggacgtgcc caccccgccc gacgcgccgc
     2161 cgcgcacgaa aacttcggtc ggcaactcgc ccgttgacgc tgccgtcggc gcgggcatga
     2221 agcgcgtcgg ctccgactcg tcggccgccg cggcgtcggc gacgagcggc acgccgtcca
     2281 cgcggcagaa gttcagagac tcgtccgcgt aagtccggcg acactcgggg cagcgtttca
     2341 tcgggcgtcc gttatagcct ctcgcgccgt tcgggggtaa caacgccgag gcgcgcgtcg
     2401 agggcgtagc gcagggcgcg gaaatgttcg tcggggattt ctgcgacggc gccttgcaga
     2461 ccgcgtgtga cgccctcgat ctggcgctcc tcctcctgct ccacagactc gaccgcctcg
     2521 gccaccgcgc gcgggtcggg ccggggcgcg ccgcgtcgcc ggagcgcccg ctcaaggatt
     2581 ccggccgcgc cgacgaggac gttcttcgag ccgcggaagg tggcgccgac ggccgactcc
     2641 agaggcttga cgggcgcctc acggcacacg gcgtcgatgg ccgcgccgac ttcagcggcc
     2701 gtcttccgtt cgatgagacg cgctccgtcg gcgcgcgggg cgagcgcgtg gtcgatgaga
     2761 tagccgtgat gataggtgcg gctcgtcagg tcggcggcgc gcttccattc gaggaagaag
     2821 aaaatcttgc gaaagacttt cttgaacgga taaagaagcg cctgccccag acatccgccg
     2881 aggcacccgt cgccgccgcg ctcggtcgtc agcgcctcga cctcttcgtg actgagctgc
     2941 caaccacggc cggcggccag ctcccgcacc atccgccgca gcaggtgact cttgacgagg
     3001 tcgtccacca tggggacggg gatgagcggc gtcagcccga cgagcacggc gtgcgtcgcc
     3061 agaagtttcc ggctcgcgcc ctcgggcgcc ggcgccgcgt cggggcgtcc tgtttcatca
     3121 gacattattc accttgcgaa gtttccgaga tgattcacgc gcggccgtct tccttgcgga
     3181 cgacaatata tcacgccgca tttcatggcc gcgggcgcgc ccgccgccga cggggcgagc
     3241 gcgcacctgc ttgacacccg cgcgcccttt ccatagtctt caaatcgggg acgaccactc
     3301 cgacccgcca acattaaatc cgggctaaac cacctagaat aacgtttacc agaatgatta
     3361 aagatcaatt caaggaactc atcgagacga agcgcgcgcg cgtcggcgtc gtcggtctcg
     3421 gctacgtcgg tctgccgctc gtcgtcgagt tcgcgcagca gggcttccac ggcgtcggct
     3481 tcgaggtcga tgcgaagaag gccgacacga tcaacgccgg cgaaagctac atcggcgacg
     3541 tatcttcgtc ggccgtcaaa gagatggtcg aggccggaag gcttttggcc acgaccgact
     3601 tcgaccgcct cgcggagtgt gacgccatca tcatctgcgt ccccacacct ttgcggaaga
     3661 cgaaggagcc ggacatctcc tacatcctcg ccgcctccgg cgagattcag aagcgcctgc
     3721 gccgtgggca gctcgtcgtg ctggaatcga cgacctaccc cggcaccacc gacgaggtgc
     3781 tcctgccgat gttcacggag acgggcctcg ggctggataa agacttcctg ctggccttct
     3841 cgcccgagcg cgtcgatccc ggcaacccgc agtttaagac gcacaacatt ccgaaggtcg
     3901 tcggcggctg cacggacgac tcgaccgagg cggcggccat gctctacggc cagatcgtcg
     3961 cgagcgtcca ccccgtcacc tcggcgcgcg tggccgaggc ggcgaagctt ctggagaaca
     4021 ccttccgcgc cgtcaacatc ggcatggcca acgagatggc gcggctgtgc tacaccctcg
     4081 gcatcgacac ctgggaggtc atccgcgccg ccgccaccaa gcccttcggc ttcatgccct
     4141 tctaccccgg ccccggcttg ggcggccact gcatcccctt agacccgcac tatctttcgt
     4201 ggaaggcgcg ccagcacggc ttcgagtcgc gcttcatcgg actggccgag gaggtcaact
     4261 cgcgcatgcc cgaccacgtc gtccgcctca tcagcgacgg cctcaacgac gagcgcaagt
     4321 ccttaaacgg ctcgcgcgtg ctcatcctcg gcgtcgccta caagaaggac atcgacgacg
     4381 tgcgcgagtc gccggcgctt tccatcatcg accgcctgcg cgccaaaggg gcggacgttc
     4441 gctaccacga cccctacgtc gcggaactgc gcttcgacga cgcgcacacc gaaggctcgg
     4501 gcgagccgat tcagtccgtg ctcctgacgg acgacgagct gcgcgcttcg gactgcgccg
     4561 tcatcgtcgc cgaccacacg ggcgtggact acgagcgcgt ctgcgcgctc tccaagctca
     4621 tcatcgacac ccgcaacgcc ctcggcgggg agctgcgccg gaagagctgc gcgaagatca
     4681 tcaggctgta gggagccgga aataagaaca cggataagac ggatgagcac ggattcacac
     4741 ggattgaatc cgtgctcatc cgttttatcc gtcctatccg tgttccacga cgccggcccg
     4801 ccaggcggcc acacactgcg cgagcctctg cgcgaatggg cggggctgcc agccgggcgc
     4861 gagcgcgcgc agacgcgtgg tgtcggcgcg ccagcgttgc gggtcgccgg ggcgctccag
     4921 agcgcggcaa cgcacctcct tgccgggcgc gacgagcgcg cgcacgcgct ccgccaactc
     4981 caacacgttc gtctcctcgc cgctcgcgac gttcacgacg cgaaggcgtc cggcgtccgg
     5041 ttctgcttcg gccacctcgc gctcgatcag tcgcagcagc gcgtcggccg cgtcgtcgat
     5101 gtgtagatag tctcttgtct cggcgcccgt gccttcgagc cagacttccc gcgcggggcc
     5161 ggcgagttgt ttgtacaact cccagacgag cagccgccgc tgcccctcgc cgaagacgga
     5221 gaacagccgg cagaccgtga cgcgcgcgcc gaagcactcg gcgtactcgc gcgccagcag
     5281 ctcgcaggcg gccttgtgga agccgtaagg cgagatggga ttgagcggcg cgtcttcgcg
     5341 tgcgggcagc tcggcggggc ggccgtagac ggcggcgctc gaagggaaga ggacgacggg
     5401 cgcgcggccc gagcggcgca cggcgtcgag cgtgttggcg aagctgagga gcgcggcgcg
     5461 gaggtcgtcg aggggcgaga cgaaagaggc gccgacggag gccgtgccgg cggcgtggat
     5521 gacggcgtcg ggggcgaagt cgcggacgag gggcgcgagg tcggagctaa tcacgtcggc
     5581 ctgcgcgtat gcgccgggcc acgcgggggc ggcctgcgtg gcccgcccca cgccgagcac
     5641 ctcgtgcccc gcacgcgcgg cgaagcgccc gacgcccccg ccgacgaagc cgcggctgcc
     5701 tgtgattaaa attctcaccg cttcttctca aaagaaattt acatggatgg gcaggataca
     5761 agagtgatga gtgatgagtg atgagtggta agagaaagcg tgacttgctt ccttcttatc
     5821 actcatcact catcactcat cacttcttct cgccctgccc ctacagaatt ggtttcgccg
     5881 cctgttcctg ttgtgtgttc tggccaggca gacttccggc ccggccgagt cgcatcagcg
     5941 gctcattgat acggcgctcg acgacttcgg gcgagaagtg ctcgcggacg tgcgcgtagc
     6001 cgttgtcaga gagcttctgc cacaactcgc cgtcgaggta gagacgcacg acggcgtcgg
     6061 cgaaggcgcg cgcgtccggg gtgatcatgg cgcttgtgcc gtcttcgagt cccatgcctt
     6121 cagcgccgac gggcgtggtg acgacgggca ggccgtaggc cagcgactcg ccgatcttgc
     6181 ccttgacgcc cgcgccgaat cgaatcggcg cgacgaagac gcgggagcgc tcgaagaacg
     6241 gatcaacgtc gggcacgtag ccggtcacgc gcaccgcgtc ggaggcgtag gcgctgatct
     6301 cgggggagga gttgtcgccg acgatggtga gtcgcacgcc gggcagggct tcgcgcacga
     6361 gggggaagat ttcacgcatg aagtgctgga cgccgtccga gttcgggcgg tggtagaggt
     6421 tgccgacgaa gaggaggtcg cgccgctcgt cgaatgggag gccgcgcccg tgcggcgtgt
     6481 ggatggtcgg gatgatctcg acggggcggc cgctcacctc tcgctccagc gcgcgccggt
     6541 cttcgggcga ggcgcaccag aagaggtcgc tcgcttgcgc gaggcgcgtc tcgatctcgc
     6601 ggtagcgcgc ggcctcggcg cgcgcgcgcg cgtcgccact caactcggcc tcgcgcccga
     6661 tgcggatgaa gtaggcgtcc accatgtcgt agacgatctt gatgccgggt gacgcgcgcc
     6721 gcacgtcttc gaggagcgcg tcggccactt cggggcggct gaagatggcg gcgtaggggc
     6781 gtcgctcgcg cagcagccgc gcgtaatcga cgaggctcgc cgtctcgatg ccctcgcgcc
     6841 agagcaggct ctcgtagttt gtccccgtgg ggcggttgaa tggaatgaag acggggcggc
     6901 tccagcgggc gagcgagcgg aggatgtgga acatgcgggc gctgccggcg tcgcggtcgg
     6961 gcgtgggcgt gcgctcgtcg aagacgaaga cgaagggcgt gcgccgtcgc tccgccgcgc
     7021 gctccgccgg cggcgcggcg cgg
//
LOCUS       DASVES010000352        14611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_783604, whole genome shotgun sequence.
ACCESSION   DASVES010000352 DASVES010000000
VERSION     DASVES010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783604"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2971)
                     /locus_tag="VF240_14380"
     CDS             complement(<1..2971)
                     /locus_tag="VF240_14380"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7176448.1"
                     /translation="MNSRHTSFGRRVATLLGTLVLPLLVCASAHAQGTSSVRGTIADA
                     QGNVVAGATVTLTNIETNAARTATTSDEGGYSFELIQPGNYQLEAEATGFKKGVVTDV
                     RALVAKPTEVNIALEVGAVTESVTVAAGSGEVLLNTQDATLGNNFVAEQIAQLPLEAR
                     NVATLLTLQPGVTRGGSVAGSRADQANVTLDGVDINEQQSNQIGAVSSNSTDPSTNTV
                     LRLNSEAVEEFRVVTSNANAQMGRSSGAQVSVVTKSGGNAFSGSAFWFHRPTILTAND
                     FFNNRSGVERPALIRNTYGGSIGGPIKQDKLFFFYSYEARKDRSQTSAVRVVPLASLG
                     RGELRFVNPSGGITTLNAAQIATATNGVGVSPISQAALAAAAARYPANEFGTQGDSPN
                     GTTLLNTAGYRFNVSQPIDLNSHVARFDYNLTDRQTLFVRGNAIHDLIGGVPWFPDTP
                     SPDTWSHPSGLAVGHTWTISNRFVNNFHYGMTRQAFSQQGDAGKNEVYFRFIFAPVLD
                     SRTLTRITPTHNITDDFSWIAGNHSAQFGTNIRIIRNKRTSYSNAFDTAYTNPSYYSA
                     SGTILSDAINGVSPIGSGQASVVQNSLAALLGRLSTYTARFTFDKDGTLLEPGAPTER
                     EFATEEYDFYVQDTWKFRQNLTFTYGLRYGLSMPIYETQGFETKPNIALSEYLRRRVA
                     ASEQGQNYIEPLVVDLSGPANNRETMYPLDKNNFQPRVGVAWSPNFKSGFLGAVFGGE
                     DQSVIRGGFAVTNDYIGQQLAVRFDTVNSLGFSSSQTSPANFCNFTTRVCPLFTGYAQ
                     DVRSLPIIVAPTGLTFPQQQPADNSLRIESSLDEEIISPTSYSWNLTWERKLPKGLVI
                     QASYIGRDAKNLLAARDVMMPNNLRDPQSGMDWYTAAGTIEDIRRNLAAQGITAASPR
                     AAQLAAINAVAPIAYFENLFGSIPGIGTTLCGSRGAGMNATRAVLCDALVFNRNDWTT
                     TQAELDFALLDAGRDQLF"
     gene            complement(3539..4177)
                     /gene="ribA"
                     /locus_tag="VF240_14385"
     CDS             complement(3539..4177)
                     /gene="ribA"
                     /locus_tag="VF240_14385"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX7176449.1"
                     /translation="MREEQTIDCPWSRQPLTVERVSSARLPTEVGDFLIAGYRSLTSD
                     EEFVALYKGDIRPELPTLARIHSQCLTGDVFGSTKCDCGRQLRRAMELIEGEGRGAIV
                     YQMQEGRGIGIINKIRAYALQDEGADTVEANERLGLAADLREYRQCAEVLFDLGLCQV
                     KVLSNNPLKLRAMEEAGLRVVERVSIEVEAADPAMHYLRTKKEKMGHLLEIE"
     gene            4476..5528
                     /locus_tag="VF240_14390"
     CDS             4476..5528
                     /locus_tag="VF240_14390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7176450.1"
                     /translation="MKEDERHAAGDAAAGEARDTVHGAADDSHIHTTDVAPALTGGRV
                     RRPVLVFLRGELLAAPIPLERDEVVLGRALEADVRVDDARASRLHASITTERDPATGE
                     ARFRVRDLQSTNGTIVNGERITDALLQDGDKLLVGEHLIRFDLLDDIDREFQRHIHRL
                     LAHDELTGLLTSKSFFSELRREAARAEAETRPFCVLMMDIDHFKEVNDRHGHLVGSQT
                     IADVAAVIKDAMRAGDVAARFGGEEFAAFLLNADCAQGIVAAERVRTSVESHVFSSKR
                     RGSSAEIDGPLRLTISVGVAAYPEDARDPIELVELADTALYRAKNSGRNLVSAYCAPQ
                     SEATARGESRLAPRAE"
     gene            5943..6599
                     /locus_tag="VF240_14395"
     CDS             5943..6599
                     /locus_tag="VF240_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176451.1"
                     /translation="MISRLNLASRPFRNRTLPWTTAVVVSLVSLLVIVYVLTEGSRAR
                     AQADAAERTVAQMREERKVIEQQASAVRQELPPDQAEVLEAAHALVSRKTFSWSQLFS
                     DLESAMPESVRLQRINVREVSQRAGQTRAELEMTVVGRSPDDVTTMMTEMSRLGSFAA
                     TPMTENFKSERGERGYEWTLRVSYVQRLKSSGGGDAAVESADAAAREASGAERAEARR
                     "
     gene            6599..7399
                     /locus_tag="VF240_14400"
     CDS             6599..7399
                     /locus_tag="VF240_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176452.1"
                     /translation="MSRETPQARLDARAGVRRFIVSRQRSRLGVPEIVALGSAALLLL
                     AALASYMLFLRPQRARSEALREEQGRLERQLQTARSEGQQSESTQASVERILVSLRDF
                     EEQHLGGSTPNGTTVVIKKLNDLIRRHNLRISGGLAFTPFETGGAPGAQPRSTGVSKP
                     IQAIYPGIGISISVEGRYADLRRFIRDVETDERFVVINSIELEGITDTAARNFSAVEP
                     PAGEGPAAPSSGPTARGTFVSLRLDMAAYFRRPAGAASDDATTPGATR"
     gene            7426..8346
                     /locus_tag="VF240_14405"
     CDS             7426..8346
                     /locus_tag="VF240_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176453.1"
                     /translation="MALFEGKTPAERNKTIAALAFGLIALILLGRMFFGSSGSGTTTT
                     TNANRRQAARNASTAPGAAQQDDRIDTAPRPVVYERVVYDGGDAGRNIFAFFARPQST
                     SVALPAISPTPPPTPTPPPPLLLASLAPPNVYAQTGEFALKLSGDKFTAAVRVYVDNQ
                     EVPTRYDGAQQLTATVPSSLITAPGNRQITVRTPDGVLYSNQASLNVMQPPVPTYTYI
                     GFISRPRTSETAVLKDQKNDLLSVQKNDVVGGRFQVMGITARSVELQDKDLKIKHTLP
                     YIAGVPGGPIPRGAVPPPPPPSDDGGDEEP"
     gene            8350..9045
                     /locus_tag="VF240_14410"
     CDS             8350..9045
                     /locus_tag="VF240_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176454.1"
                     /translation="MNLRTPTLTTRPTHGARVVVNAGVAAASAPGQCGYALVALLALM
                     TIIMLAATAAAPNIIQQSQREAERESIIRGEEVADAIESFVRLTNRPPRSMEELLEGA
                     NPQGRTKKVRVLRSYAARDPLARASDGEWRLVQPRGKEIVEFQQALMLYMEGRPLPPS
                     PEAWKNQARVQLTGVTDLGRGSGGGDSGGEDTSANANVPFIGVASRSRRESVVTYFGI
                     ERHDEWVFTPIYR"
     gene            9613..10083
                     /locus_tag="VF240_14415"
     CDS             9613..10083
                     /locus_tag="VF240_14415"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX7176455.1"
                     /translation="MLHVALVEPEIPPNTGNVARLCAATRTPLHIVGVAGFRLDDRAV
                     RRAGLDYWPEVDLRRHRDLSALYAALPSARFLYLTTKAEKPYYEWEFADGDCLVFGRE
                     TKGLPEDLLRANWGRCLTIPMPNARVRSLNLATSVAVVLYEALRQTGALESRGG"
     gene            10202..12010
                     /gene="typA"
                     /locus_tag="VF240_14420"
     CDS             10202..12010
                     /gene="typA"
                     /locus_tag="VF240_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7176456.1"
                     /translation="MLREDIRNIAIIAHVDHGKTTLVDAMLKQSGTYRDNEQVIERVM
                     DSMDLERERGITIMAKNTSVRYGDVKINILDTPGHADFGGEVERVLKMVDGVMLLVDA
                     AEGPLPQTRFVLRKALEAHLPAIVVVNKIDRQDARPEEVVDEIYDLFIDLDADGEQID
                     FPILYAVSREGVAKKNLSDDSRTLRPLFDQIVETIPAPKALRADALQLLVANLDYSEY
                     VGRLAIGRIFSGEIAAGDTVAVAKRDRTLQKLRVKELYVFEGLKREKVERAAFGEIVA
                     LAGMEGIEIGETITSADDPRPLPVIAVDEPTISMIFGVNNSPFAGTEGKYVTSRQIME
                     RLDRELLSNVAIRVEPTESPDQFKVSGRGELQLAILIEMMRREGYELQVSKPEVITHH
                     EGGQLVEPIEQVVVDCPEEFIGVVTEALGRRKGQMTKMVNHGTGRVRLEFETPSRGLI
                     GFRNEFLTETKGTGLLNTLFLRWGPGQGAMRGRTTGSLVSDRTGEATTFALYNLQERG
                     TMFIRPNTRVYEGMVVGENARAVDLDVNIIKEKKLTNMRAASADEAMRLVPVKDLSLE
                     QALEFIADDELVEVTPASIRLRKRVLQANRRPKKEA"
     gene            complement(12285..12659)
                     /locus_tag="VF240_14425"
     CDS             complement(12285..12659)
                     /locus_tag="VF240_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7176457.1"
                     /translation="MSSTKRRILCAESNKDVGELIARLLENKGYEVETVQTAAQCMSL
                     AREGGFDLYILNDSYIDADSIELCQQLRALDSAIPVLLFSLDSSGYDRRKALGSGVQL
                     YVSKTSDFVALVQTIDQLMQSR"
     gene            complement(12759..13172)
                     /locus_tag="VF240_14430"
     CDS             complement(12759..13172)
                     /locus_tag="VF240_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7176458.1"
                     /translation="MPPDQRRVLCAEPSEDICTLLSVMLGHSGYEVVSASSVHDCLEL
                     ARTQRFDLYMLDDDYIDGTAVELCKQLRVLTPETPILFFSTAALERDRQQGLAAGASA
                     YLTKPDDIFEIVETVNSILLPRAAGRAAAGDAESP"
     gene            complement(13358..13558)
                     /locus_tag="VF240_14435"
     CDS             complement(13358..13558)
                     /locus_tag="VF240_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176459.1"
                     /translation="MSVRDDTPEEGAPERPAGNDAAAAPRPAPERAPLGYYYDDGTNY
                     ETYDPRAEEERESQDGETGAKL"
     gene            13608..>14611
                     /locus_tag="VF240_14440"
     CDS             13608..>14611
                     /locus_tag="VF240_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX7176460.1"
                     /translation="MKPFRLRATLHTLLILTSLLLPRAAAGQAQRQQQQPARFELTVD
                     SIMRGPDLVGYAPTQVVWSQDGARVYFRWKRAGETRLKEPDTYVVAADGTGLRKLSEE
                     EARRQAPPSGGELSKDKKMSVFVEDGDVFLYDHTTAARRQVTRTTDAESNARFTRDGR
                     AVYFTRQNNLFVLSLDGGSLEQLTDVRTGGSGGAAEQRAAGGRGAQRQGGSSGGDDAQ
                     RRGTESQEVLKREERALLEAVRERAEQREEQEAKRKRRETRKPFNVPTGQTVANLTLS
                     PDGRFVVAAVSEPASGAKNTIVPNYVTESAYTEDISSRTKVGDTQGRSRLAVISVETG
                     "
BASE COUNT         2560 a         4767 c         4884 g         2400 t
ORIGIN      
        1 agaagagctg gtcgcggccc gcgtcgagca gggcgaagtc gagttcggcc tgcgtcgtcg
       61 tccagtcgtt gcggttgaag acgagggcgt cgcagaggac ggcccgcgtg gcgttcatgc
      121 ccgcgccacg cgacccgcac aacgtcgtgc cgatgccggg gatactgccg aacaggtttt
      181 cgaagtaggc gatcggggcg acggcattga tggccgccag ttgcgcggcg cgcggcgacg
      241 cggccgtgat gccctgcgcc gcgaggttgc ggcggatgtc ttcgatggtt ccggcggccg
      301 tgtaccagtc catgccggac tgcgggtcgc ggagattgtt cggcatcatc acgtcgcgcg
      361 cggcgaggag gttcttcgcg tcgcgcccga tgtaggaggc ctgaatcacg aggcccttcg
      421 gcagcttccg ctcccacgtg aggttccaac tgtagctggt gggcgagatg atctcctcgt
      481 cgagagagga ctcgatgcgc aggctgttgt cggcgggctg ctgctgcggg aaggtcagcc
      541 ccgtgggcgc gacgatgatc ggcaggctgc gcacgtcctg agcgtagccc gtaaagagcg
      601 ggcagacgcg ggtggtgaag ttacagaagt tggccgggct cgtctggctc gaagagaagc
      661 cgagcgagtt gaccgtatcg aagcggacgg cgagctgctg accgatgtag tcgttggtga
      721 cggcgaagcc gccgcggatg accgactggt cttcgccgcc gaagacggcg ccgaggaagc
      781 cgctcttgaa gttgggcgac caggcgacgc cgactctcgg ctggaagttg ttcttgtcga
      841 gcgggtacat cgtctcgcgg ttgttggccg ggccggagag atcaaccacg agcggctcga
      901 tgtagttctg tccctgctcg gacgccgcca cgcggcggcg caggtactcg gagagcgcga
      961 tgttcggctt ggtctcgaac ccctgcgtct catagatcgg catgctcagg ccgtagcgca
     1021 ggccgtaggt gaaggtcagg ttctggcgga atttccacgt gtcctgcacg tagaagtcgt
     1081 actcctcggt ggcgaactcg cgctcggtgg gcgcgccggg ttcgaggagc gtgccgtcct
     1141 tgtcaaaggt gaagcgcgcc gtgtaggtcg agaggcggcc gaggagcgcg gccagcgagt
     1201 tctgcaccac ggaggcctgg ccgctgccga tgggtgagac gccgttgatg gcgtccgaga
     1261 gaatcgtccc cgaagcggag tagtaggacg ggttggtgta ggccgtgtcg aaggcgttgg
     1321 agtacgacgt gcgcttgttg cggatgatgc ggatgttggt gccgaactgc gccgagtggt
     1381 tgccggcgat ccacgagaag tcatccgtga tgttgtgcgt cggagtgatg cgcgtgagcg
     1441 tgcgcgagtc gagcacgggc gcgaagataa agcggaagta tacttcattc ttccccgcgt
     1501 cgccctgctg cgagaatgcc tgtcgcgtca tgccgtagtg gaagttgttg acgaagcggt
     1561 tgctgatcgt ccacgtgtgg ccgacggcga ggcccgaagg gtgcgaccag gtgtcggggc
     1621 tcggcgtgtc ggggaaccat gggacgccgc cgatgaggtc gtggatggca ttgccgcgga
     1681 cgaaaagggt ctgccggtcg gtcaggttgt agtcgaagcg cgcgacgtgc gagttgaggt
     1741 cgatgggctg cgaaacattg aagcggtagc ctgccgtgtt gagtagcgtc gtgccgttcg
     1801 ggctgtcgcc ctgcgtgccg aattcgttgg ccggataccg ggcggcggcc gccgccagcg
     1861 cggcctgcga tatggggctg acgcccacgc cgttggtggc tgtggcgatc tgggcggcgt
     1921 tgagcgtcgt gatgccgccc gacgggttga cgaaacgcag ctcgccgcgc ccgaggctcg
     1981 ccagcgggac gacgcgcacg gcgctcgtct ggctgcggtc tttgcgggcc tcatagctgt
     2041 agaagaagaa gagcttgtct tgcttgatgg ggccgccgat ggagccgccg taggtgttgc
     2101 ggatgagcgc cgggcgctcg acgcccgaac ggttgttgaa gaagtcgttg gccgtcagga
     2161 tggtcgggcg atggaaccag aaggccgagc cggagaaggc gttgccgccc gacttcgtga
     2221 cgaccgagac ctgagcgccc gaggagcggc ccatctgcgc gttggcgttg gaggtgacaa
     2281 cgcggaactc ttcgacggcc tccgagttga ggcggagcac ggtgttcgtc gatgggtcgg
     2341 tgctgttgct ggagaccgcg ccgatctggt tgctctgctg ctcgttgatg tccacgccgt
     2401 cgagcgtgac gttggcctgg tcggcgcgcg agccggcgac cgagccgccg cgcgtgacgc
     2461 cgggctggag cgtcaggagt gtggcgacgt ttctcgcttc gagcgggagt tgggcgatct
     2521 gctcggcgac gaagttgttg ccaagcgtgg cgtcctgcgt attcaggagg acctcgcccg
     2581 agccggccgc gaccgtcact gattcggtga cggcgccgac ttcgagcgcg atgttgacct
     2641 cggtcggctt ggcgacgagg gcgcgcacgt ccgtgacgac gcccttcttg aagccggtcg
     2701 cctcggcttc gagctggtag ttgccgggct ggatcagctc gaaggagtag ccgccctcgt
     2761 cgctggtggt ggccgtgcgc gcggcgttcg tttcaatgtt ggtgagcgtg acggtggcgc
     2821 ccgcgacaac gttgccctgc gcgtcggcga ttgtgccgcg cacggaggaa gtgccctgcg
     2881 cgtgcgcgct ggcgcagacg agtaggggta ggacgagcgt gccgagcaga gtggcgacgc
     2941 gacgcccgaa ggacgtatgc ctgctgttca tcgttccctc caaagttggg taagagaccc
     3001 ggcgcggccg ctcaggggcg gaaccggcgg gcggggggtg agacgtaaat gtcatcacgg
     3061 tctcaatctt atttggcttg agttaagact cggaccgggg cgcctggaac ccgtgcgggt
     3121 ctcgcgcgcg cgccgcgctc cgattgaaca ccctcaggat gagaatgatt ccgggtattt
     3181 aagggctatg cttgtcgcgg gcctgaacgg ctccggctgt cggtctgctc tcgtccaccg
     3241 cttgcggccg tggggggcgc gttcgcgccc ttcaggtcaa taatttggac gccgaaggtt
     3301 cgggcaactg tcgttccacg tcaccctccg ccatcatgcg gctaataaca tggtaagact
     3361 caggacttta ggcgacaccg cccggcggaa ggcggggcat tataatccaa ataatccaaa
     3421 aacctagagt tgtgattcat atttttggat agagacgcgg cggcgtgaaa agcgcgggcg
     3481 ggcggggcgg gccggaaaga attcagaagt ggagggacga agccgccgct cggctccctc
     3541 attcaatctc cagcaggtgg cccatcttct ctttcttcgt gcgcaggtag tgcatggccg
     3601 ggtcggccgc ctcgacctcg atggagacgc gctcgacgac acgcaatccc gcctcctcca
     3661 tcgcgcgcag cttcagggga ttgttcgaca gcaccttgac ctgacacagc cccagatcga
     3721 agagcacctc ggcgcactgc cgatactcgc gcaggtcggc ggccagtccc agacgctcgt
     3781 tggcctccac cgtgtcggcc ccttcgtctt gcagggcgta ggcgcgaatc ttgttgatga
     3841 tgcctatccc gcggccctcc tgcatctggt agacgatggc gccgcggcct tcgccctcga
     3901 tcaactccat ggcgcgccgg agttgccggc cgcagtcgca cttggtggag ccgaagacgt
     3961 cgcccgtcag gcattgcgag tggatgcgcg caagcgtcgg cagttcgggc cggatgtcgc
     4021 ccttgtagag cgcgacgaat tcctcatcgc tcgtgagcga gcggtagcct gcgatcagga
     4081 aatcgcccac ctcggtcggc agccgcgccg acgagacgcg ctcgaccgtc agcggctgcc
     4141 gcgaccacgg gcaatcaatg gtctgctctt ctctcaaatc tggaactcct cggcctgccg
     4201 tgatgactca ctgcgcgcgg cctcacgcgc ccgacacctc gcgcccggcc gcccgcgccc
     4261 ggactggttt cgccgcgagg tgttacgagt ccggcaagta tagaagagcg gcgggcgggc
     4321 cacaagacag ggagaagacg cgtcaagcgt caagcgtcaa tcgtcaagcg tgtttgagca
     4381 gcattcggct tgagcagcga atcccgaatc aacgtccgca gcaacccgcg cgatttacga
     4441 tttaccattc acgatttacg ctttaagatg cccgcgtgaa agaggacgaa cgccatgccg
     4501 cgggcgacgc cgccgccggc gaagcacgcg acaccgtcca cggggccgcg gacgacagcc
     4561 acatccacac gactgatgtc gcccccgcgc tgacgggcgg acgcgtgcgc cggcccgtgc
     4621 tcgtcttcct gcgcggcgag ctcctcgccg cccccatccc tctggagcgc gacgaggtcg
     4681 ttttgggccg cgcgctcgaa gccgacgtgc gcgttgacga cgcccgcgcc tcccgcctcc
     4741 acgcctcgat caccaccgag cgcgaccccg ccacgggcga ggcacgcttc cgcgtccgcg
     4801 acctccaatc gaccaacggc accatcgtca acggcgagcg catcaccgac gccctgctcc
     4861 aggacggcga caagctgctc gtcggcgagc acctcatccg cttcgacctc ctcgacgaca
     4921 tcgaccgcga gttccagcga cacatccacc gtttgctcgc gcacgacgag ctgacgggcc
     4981 tgctcacgag caagtcgttc ttctcggagc tgcgccgcga ggccgcgcgg gccgaggccg
     5041 agacgcgccc cttctgcgtc ctgatgatgg acatcgacca cttcaaggag gtcaacgacc
     5101 gccacggcca cctcgtcggc agccagacca tcgcggacgt ggccgccgtc atcaaggacg
     5161 cgatgcgcgc cggcgacgtg gccgcgcgct tcggcggcga ggagttcgcc gcctttctcc
     5221 tgaacgccga ctgcgcgcag ggcatcgtcg ccgccgagcg cgtccgcacc tcggtcgaga
     5281 gtcacgtctt ctcgtccaag cgccgcggct cgtcggccga gatcgacggg ccgctacgcc
     5341 tcaccatcag cgtcggcgtc gccgcctacc ctgaagacgc gcgcgacccc atcgagctgg
     5401 tcgaactcgc cgacacggcc ctctaccgcg ccaagaactc cgggcgcaac ctcgtcagcg
     5461 cctactgcgc gccgcagtcc gaagccacgg cccgcggcga gtcgcgcctc gccccgcgcg
     5521 ccgagtgaag gctgcgccct cttggaaaca ccgacgcgca catctgagag gaaaccattg
     5581 cggcgcgggc aaggtcgtca actcgcgcgc cgtcagccag gcgacgggca cgacgcgccc
     5641 gcggccgtaa acgaagtcag ggaaaagtaa aaaggcagaa gtgagagggc aaaggagggc
     5701 cgcgcggcct ctttttgccc ctcgcttttt tgcaaccgga cgcgccgcgc aacgtgtaaa
     5761 gctgccggct cggggcaggc cgcgcgccgc gcggcgcgcg cgcggattcc acggccttat
     5821 cggccttttg ccttttgcct ttcctgcgcg ctatgcttgc ttgacggcga ggccgtgtga
     5881 agtagacgcc ccgactctta agcgccgcct ccgcaccgga actcggaaag acgaagactc
     5941 cagtgatctc cagactcaat ctcgcgagcc gccccttccg caaccggacg ctgccgtgga
     6001 cgacggccgt cgtcgtcagc ctcgtctcgc tgctcgtcat cgtctacgtg ctgacggaag
     6061 gctcccgcgc ccgcgcgcag gccgatgcgg ccgagcgcac ggtggcgcag atgcgtgagg
     6121 agcgcaaggt catcgagcag caggcgtcgg ccgtgcgtca ggagttgccc ccggatcagg
     6181 ccgaagtcct cgaagccgcc cacgccctcg tcagccgcaa gaccttctcc tggtcgcaac
     6241 tcttctccga cctcgaatcg gccatgcccg agagcgtgcg cctccagcgc atcaacgtgc
     6301 gcgaagtctc gcagcgcgcc gggcagacgc gcgccgaact cgaaatgacc gtggtgggcc
     6361 gcagccccga cgatgtcacc accatgatga ccgagatgag ccgcctcggc tccttcgccg
     6421 ccacgcccat gacggagaac ttcaagtcgg agcgcggcga gcgcggctac gagtggacgc
     6481 tgcgcgtgag ctacgtgcag cgcctgaaaa gttctggcgg cggcgacgcg gccgtcgaga
     6541 gcgcggacgc tgcggcgcgg gaagcgtcgg gcgcggagcg cgcggaggcg cgccgttaat
     6601 gtcgagagag acgccgcagg cgagattgga cgcgcgcgcg ggagtgaggc gattcatcgt
     6661 ctcgcggcag cggagccgtc tcggcgtccc cgagatcgtg gcgctcggct cggccgcgct
     6721 cctcctgctg gcagcgctcg cgtcttacat gctcttcctc cgcccgcagc gagcgcgctc
     6781 cgaggcgctg cgtgaagagc agggaaggct ggagaggcag ttgcagaccg cgcgctcgga
     6841 ggggcagcag tccgagagca cgcaggcgag cgtcgagcgg attctcgtct cgctgcgcga
     6901 cttcgaggag cagcacctcg gcggcagcac gccgaacggc acgaccgtcg tcatcaagaa
     6961 gttgaacgac ctgatccgcc gccacaacct gcgcatcagc ggcggcctcg ccttcacgcc
     7021 cttcgagacc ggcggcgccc ccggcgcgca gccccgcagc accggcgtca gcaagccgat
     7081 tcaggccatc taccccggca tcggcatcag catctcggtc gagggccgct acgccgacct
     7141 gcgccgcttc atccgcgacg tggagacgga cgaacgcttc gtcgtcatca actccatcga
     7201 actcgaaggc atcacggaca cggcggcgcg caacttctcg gccgtcgagc cgccggcggg
     7261 cgaaggccct gccgccccgt cgtccgggcc gacggcgcgc ggcaccttcg tctctctgcg
     7321 gctcgacatg gccgcatact tccgtcgccc cgccggcgcc gcctcggacg acgcgacgac
     7381 tccgggcgcg acgcgctgac gccgaggtgc gcgacaggat ttttcatggc actctttgaa
     7441 ggcaagactc ccgccgagcg caacaagacc atcgccgcgc tggccttcgg tctcatcgcc
     7501 ctcatcctgc tcggccgcat gttcttcggc tcatcaggct cgggcacgac gacgaccacg
     7561 aacgccaacc gccggcaagc cgcgcgaaac gccagcaccg caccgggcgc cgcgcagcag
     7621 gacgacagga tcgacacggc gccacggccc gtcgtctacg agcgcgtcgt ctacgacggc
     7681 ggcgatgccg ggcgcaacat cttcgccttc ttcgcccgcc cgcagtctac ttccgtcgcc
     7741 ctgccggcca tcagcccgac gcccccgcct acgccgacac cgccgccccc gcttctcctc
     7801 gcctcgctcg cgccgccgaa cgtctacgcg cagaccggcg agttcgccct caagctctcc
     7861 ggcgacaagt tcacggccgc cgtgcgcgtc tacgtcgata accaggaagt gccgacgcgc
     7921 tacgacggcg cgcagcaact caccgccacc gtcccgtcct ccctcatcac cgcgccgggc
     7981 aatcgccaga ttaccgtccg cacgcccgac ggcgtgctct actccaatca ggcgtcgctc
     8041 aacgtgatgc agccgcccgt gccgacctac acctacatcg gcttcatcag ccgcccgcgc
     8101 accagcgaga cggccgtcct caaggatcaa aagaacgacc tgctcagcgt ccagaagaac
     8161 gatgtcgtcg gcgggcgctt ccaggtcatg ggcatcacgg cgcgctccgt cgaactccag
     8221 gacaaagacc tcaagatcaa gcacacgctg ccctacatcg cgggcgtgcc cggcggcccc
     8281 atcccgcgcg gcgccgtccc gccgccgccg cccccctccg acgatggcgg cgacgaagag
     8341 ccataagccg tgaacctccg aacaccaaca ctgaccaccc gccccacgca cggcgcgcgc
     8401 gtcgtcgtga acgccggcgt cgccgcggcc tcggcgcccg gccagtgcgg ctacgccctc
     8461 gtggccctgc tcgcgctgat gacgatcatc atgctcgcgg cgacggccgc cgcgccgaac
     8521 atcatccagc agagtcagcg cgaggcggag cgcgagtcaa tcatccgcgg cgaggaggtc
     8581 gccgacgcca tcgagagctt cgtgcgcctg acgaaccgcc cgccccgctc catggaagaa
     8641 ctgctcgaag gcgccaaccc gcaggggcgc accaagaaag tccgcgtgct ccgcagctac
     8701 gccgcgcgcg acccgctcgc gcgcgccagc gatggtgagt ggcgtctcgt tcagccgcgc
     8761 ggcaaggaga tcgtcgagtt ccagcaggcg ctcatgctct atatggaggg gcgtccgttg
     8821 ccaccgtcgc ccgaggcatg gaagaatcaa gcgcgcgtcc aactcacggg cgtcaccgac
     8881 ctcggccggg gcagcggcgg cggcgactcg ggcggcgagg acacttccgc caacgccaac
     8941 gttcccttca tcggcgtcgc cagccgcagc cggcgcgaat ccgtcgtcac ctacttcggc
     9001 atcgagcgtc acgacgagtg ggtcttcaca cccatctacc gttagccttc accccgaaaa
     9061 aattcactca cttcgcgcac gcgcactccg actgaagcgc gtgagaccgg catgagacgc
     9121 gactctccgc gagcgcgtcg cacgcgtcgc gcgaaaaatt ttcacataag ttattgacaa
     9181 gtgtttgtaa tatgtttata tatccaacat gttgtgcgcc aaacactctg ggccacaagg
     9241 tgaaagttga cacagtatct tgtggtcaag acctaaactg aaaaggagca gctacaactt
     9301 atggcaacta agaaggcagc taagaagagc ggagcgaagg gcggagcaaa gaaggctggc
     9361 gccaaaaagg gcgcagcgaa gaagtccggc tcgaagaagc gctaacaacc cggttcgaac
     9421 aagcggtaac tttaaccttc aactgcgcac acaaaactct gcgcccctcc gggggcttta
     9481 caaaaggacg gcgattgcat cagatgcacg ccgtcctcat tcttttagaa gaggtcagag
     9541 gtcagaggtt agataaaaac taatccgtct ttaactgacc tctaacctct gacctctgtc
     9601 ctctgttttt ttatgctcca cgtcgcgctc gtcgagccgg agataccgcc gaacaccggc
     9661 aacgtcgcgc gcttgtgcgc cgcgacgcgg acgcccctgc acatcgtcgg cgtcgcgggc
     9721 ttccgcctcg acgaccgcgc cgtgcgccgc gccggcctcg actactggcc cgaggtcgat
     9781 ctccgccgcc accgcgacct gagcgcgctc tacgccgccc tgccctccgc gcgcttcctc
     9841 tatctcacga ccaaggccga gaagccctac tacgagtggg agttcgccga cggcgattgc
     9901 ctcgtttttg gccgggaaac taagggtttg cccgaggatt tgctgcgcgc gaactggggg
     9961 cgttgcctga cgattccgat gccgaatgcg cgcgtgcgca gcctcaacct cgccacctcg
    10021 gtcgccgtcg tcctctacga ggcgctcagg cagacggggg cgctcgaaag ccgcggcggg
    10081 tgacaggcga tgtgcgacag gttagaatcc ttccttaagt cgtcatcatc aaacatctac
    10141 ttccgttttt tccggcgtgt gagggatgag agtcttatcc tgtcacacgt catgaggttt
    10201 tatgctcaga gaagacattc gcaacatcgc tatcatcgca cacgtcgatc acgggaagac
    10261 caccctcgtg gacgcgatgc tcaaacagtc gggcacgtac cgcgacaacg agcaggtcat
    10321 cgaacgcgtc atggactcga tggatttgga acgcgagcgc ggcatcacga tcatggcgaa
    10381 gaacacttcg gtgcgctacg gcgacgtgaa gatcaacatc ctcgacacgc cgggccacgc
    10441 cgacttcggc ggcgaggtcg agcgcgtcct gaagatggtg gacggcgtga tgcttttggt
    10501 ggacgccgcc gaggggccgc tgccgcagac gcgcttcgtg ctgcgtaagg ccctcgaagc
    10561 gcacctgccg gccatcgtcg tcgtcaacaa gatcgaccga caggacgcgc gccccgagga
    10621 ggtggtcgat gaaatctacg acctcttcat cgacctcgac gccgacggcg agcagatcga
    10681 ctttcccatc ctctacgccg tctcacgcga aggcgtggcg aagaaaaatc tctccgacga
    10741 ctcgcgcacg ctgcgcccgc tcttcgacca gatcgtcgag acgatccccg cgccgaaagc
    10801 tttgcgcgcc gacgccctgc aactcctcgt cgccaacctc gactacagcg agtatgtggg
    10861 ccggctcgcc atcggccgca tcttctcggg cgagatcgcg gcgggcgaca cggtggccgt
    10921 cgccaagcgc gaccgcacgt tgcagaagct gcgcgtcaag gagttgtacg tcttcgaggg
    10981 gctgaagagg gagaaggtcg agcgcgcggc tttcggcgag atcgtggcgc tggccgggat
    11041 ggagggcatc gagatcggcg agacgatcac gagcgccgac gacccgcgcc cgctccccgt
    11101 catcgccgtg gacgagccga ccatctcgat gatcttcggc gtcaacaact cgccgttcgc
    11161 cggcacggaa ggcaagtacg tgacgtcgcg tcaaatcatg gagcgcctcg accgggagtt
    11221 gctctcgaac gtggccatcc gtgtggagcc gaccgagtcg cccgaccagt tcaaggtctc
    11281 aggccgcggc gaattgcagc tcgccatcct catcgagatg atgcggcggg agggctacga
    11341 gctacaggtc tcgaagccgg aggtcatcac gcaccacgag ggcgggcagt tggtggagcc
    11401 gattgagcag gtcgtggtcg attgcccgga ggagttcatc ggcgtggtga cggaggcgct
    11461 gggccgtcgc aaggggcaga tgacgaagat ggtcaaccac gggacggggc gcgtccggct
    11521 ggagtttgag acgccgtcgc gcggcctcat cgggttccgc aacgagttcc tcaccgagac
    11581 gaaggggacg gggctgctca acacgctgtt cctccgctgg gggccggggc agggcgcgat
    11641 gcgcgggcgc acgaccggct ctctggtctc cgaccgcacg ggcgaggcga cgaccttcgc
    11701 gctctacaac ctacaggagc gcgggacgat gttcatccgc cctaacacga gggtctacga
    11761 ggggatggtc gtcggcgaga acgcgcgtgc cgttgacctg gacgtgaaca tcatcaagga
    11821 gaagaagctc acgaacatgc gcgccgcctc ggccgacgag gcgatgcggc tcgtccccgt
    11881 caaagacctg tcgcttgagc aggcgctcga attcatcgcc gacgacgaac tggtcgaggt
    11941 cacgcccgct tcgatacgtc tcaggaagcg cgtgcttcag gcgaaccgga ggccgaagaa
    12001 ggaagcataa gcctctgagg aaggtaaaag tcagagggcc gtatcgcagg cgacagcgag
    12061 ccgcggagcg gcggcagaat gtagcccacg gcgcgagccg tggggacacg tccgattaag
    12121 aaatcgaagc cccggagggg cgaaagaatc gcggccgatg cgaagcgcaa gctcacaatt
    12181 ctttcgcccc tccggggctt tttgcttttt cttcttgctg ctaccacggg ctaacgcccg
    12241 tggctacatt ctgccgccgc tccgcggctc cttggcgccg gtgattagcg cgactgcatg
    12301 agctggtcga tggtctggac gagggcgacg aagtcggagg tcttgctgac gtagagttgg
    12361 acgccggagc cgagcgcctt gcggcggtcg tagccggagc tgtcgagcga gaagaggagg
    12421 acggggatcg cggagtcgag ggcgcgcaac tgctggcaga gttcgatgct gtcggcgtcg
    12481 atgtaggagt cgttgaggat gtagagatcg aagccgccct ctcgcgcgag gctcatacac
    12541 tgggcggccg tttgcactgt ctccacctcg tagcccttgt tctccagcag gcgcgcgatc
    12601 agctcgccca cgtccttgtt cgattcggcg cagaggatgc gcctctttgt tgacgacatg
    12661 cggcccactc cggcagagag ttgcagagcg agaagacacc ttggggggtg aatttaattg
    12721 tcgggggaat gcaaatttag accgatgacg gatgcgcgtc aaggagattc tgcgtcgccc
    12781 gccgcggcgc ggccggcggc gcgcgggagc aggatggagt tgacggtctc gacgatttca
    12841 aagatgtcgt cgggctttgt gaggtaggcc gaggcgccgg cggcgaggcc ctgctggcgg
    12901 tcgcgctcga gcgccgcggt cgagaagaag aggatgggcg tctcgggcgt gagcacgcgc
    12961 agttgcttgc agagttcgac ggcggtgccg tcgatgtagt cgtcgtcgag catgtagagg
    13021 tcgaatcgtt gcgtccgggc cagctccaga cagtcgtgga cggaggaggc ggagacgacc
    13081 tcatagccgc tgtggccgag catcacggag aggagcgtgc agatgtcctc gctaggctcg
    13141 gcgcagagta cacgcctttg gtcgggaggc atggggggta ctccttcaac gctgagaagg
    13201 ggaactcttt ttgagagttg ttgaaaagag tgtccgcgcg atggtcggtt tgaagtgttt
    13261 cgctctggct tcgcgcggac agggcaacct gaacagtgag aatttagcct caaagccggc
    13321 gacgggtcaa cacttttcgc gccgcccccc gcactactca gagtttggcc ccggtttcgc
    13381 cgtcttgact ctctcgctcc tcttccgcgc gggggtcgta ggtctcgtaa ttcgtgccgt
    13441 cgtcgtagta gtagccgagc ggcgcgcgct ccggggcggg gcggggcgcg gcggcggcgt
    13501 cgttgccggc ggggcgctcg ggcgcgccct cttctggtgt atcatctcga acgctcatcc
    13561 gacaaaaatc ctccgcggcc gacagcttaa caaggactgc gacgaacatg aaacctttcc
    13621 gccttcgcgc cacgctgcac acgcttctga tcctgacttc gctcctcctg ccgcgcgccg
    13681 ccgccgggca ggcccagcgt cagcagcagc agccggcgcg attcgagttg acggttgata
    13741 gcatcatgcg cggccccgac ctcgtcggct acgcgcccac gcaggtcgtc tggtcgcagg
    13801 acggcgcgcg cgtctacttc cgctggaagc gcgcgggcga gacgcggctg aaggagccgg
    13861 acacatacgt cgtggccgcc gacggcacgg gccttcgcaa gctgtcggag gaagaggcgc
    13921 gcagacaggc gccgccgtcg ggcggcgagc tgtcgaagga caaaaagatg agcgtcttcg
    13981 tcgaggacgg cgacgtcttt ctctacgatc acacgacagc cgcgcgccgg caggtgacgc
    14041 gcacgaccga cgccgaatca aacgcgcgct tcacgcgcga cggccgcgcc gtctacttca
    14101 cgcgccagaa caacctcttc gtcctctcgc tcgacggcgg ctcgctcgaa caactgacgg
    14161 acgttcgcac tggcggcagc ggcggcgcgg cggagcaacg cgcggcgggc ggccggggcg
    14221 cacaacgaca gggaggcagc agcggcggcg atgacgcgca gcggcggggc acggaaagcc
    14281 aggaggttct gaaaagggag gagcgcgcgc tgctcgaagc cgtgcgcgag cgcgccgagc
    14341 agcgtgagga gcaggaggcc aagcgtaagc ggcgcgagac gcgcaagccc ttcaacgtcc
    14401 ctacggggca gaccgtggcg aacctcacgc tctcgcccga cggccgcttt gtcgtcgcgg
    14461 ccgtcagtga acccgcctcc ggcgctaaaa ataccatcgt cccgaactac gtcaccgagt
    14521 ccgcctacac cgaagacatc tcctcacgca cgaaggtcgg agacacgcag gggcgctcgc
    14581 gcctcgccgt catctcggtc gagacgggcg a
//
LOCUS       DASVES010000353         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1455284, whole genome shotgun sequence.
ACCESSION   DASVES010000353 DASVES010000000
VERSION     DASVES010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455284"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VF240_14445"
     CDS             <1..850
                     /locus_tag="VF240_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176461.1"
                     /translation="KVAGEAARAREEAERLAEDFDLRERADEVRTHAERAAEEATRRA
                     REAGDHLRGAGKSAGERAGEVFGGAKRYYERASRVASAGASATRLTTATTRGVSRAYD
                     WARENPKRAIMVTLSLAGGVQMGAAFPGLDAVLLGAHPHWLTHSALPVWALRKAGEKF
                     DSYLQEREELVAAGELDEAERKRVEFERKIARYVGAPLLGAFSCAAGLTMWAQILQPG
                     TVVGAPISWLIGGNPLLSGIWLFANGVICFQQGYKFFMIALRDQEDAQRIVREIRGLL
                     PAAASA"
     gene            1002..1649
                     /locus_tag="VF240_14450"
     CDS             1002..1649
                     /locus_tag="VF240_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7176462.1"
                     /translation="MMSTSVASNEMARREQVRRGRLLEYLTVGWNSLEGIIALGAGFA
                     AGSIALVGFGFDSIIEVTSGVALLWRLHLDAPERRERAEQISLKIVGVSFLALAAYVA
                     YDAVRSLITRESPEASYVGIALAALSLVVMPLLARAKRGVAAKINSRAMQADSRQTDI
                     CAYLSAILLGGLILNALLGWWWADPVAALVMTPIIVKEGVEALRGETCCDEGACH"
     gene            1649..1900
                     /locus_tag="VF240_14455"
     CDS             1649..1900
                     /locus_tag="VF240_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176463.1"
                     /translation="MLTPTTTYILLLIVQAFHLLHHRLVKRHISFAEVVSAAVLCVPP
                     FAVPLPAWLFIGAHLSLIAIQIVGSLWIRKLSPDWSKSE"
     gene            2002..2934
                     /locus_tag="VF240_14460"
     CDS             2002..2934
                     /locus_tag="VF240_14460"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7176464.1"
                     /translation="MMRKSILAFLLLSSLAGLATAQTGVVKLEGQVVCCADCWAEADR
                     TKVEYGTAEDLLKAQSCVANGDPTLLAVREGDKFTLYQLEQAKYRLPGKNWLDFVGKR
                     IAVTGAVRKKKNAGVIRVDALEVLAPSLAEQEAANVVGKEVDLILKDPFGTEQRLSAL
                     KGRIVVLNFWATYCVPCRKEMPDLAAIQNEYAALGVQVVGASADAPEDRAKVLEFVKE
                     TKVNFPVWTGASTADMLRFGLGAALPGTVIVGRDGRVAKVISGIVNQADLKKQIEAML
                     ASAEKTGAAQRQATKREQAASVKEKRSEASSVPS"
BASE COUNT          542 a          858 c          988 g          569 t
ORIGIN      
        1 caaggtggcg ggcgaggccg cgcgtgcgcg cgaagaggcc gagcgtctgg ccgaagactt
       61 cgacctgcgc gagcgcgccg acgaagtacg cacgcacgcc gagcgcgcgg ccgaggaggc
      121 gacgcgtcgc gcgcgcgagg ctggcgacca tttgcgcggc gcgggcaaga gcgcgggcga
      181 gcgcgccgga gaggtcttcg gcggcgcgaa gcgttactac gagcgcgcct cgcgcgtcgc
      241 cagcgccggc gcgagcgcca cacggctaac gacggccacg acgcgtggcg tctcgcgcgc
      301 ctacgattgg gcgcgcgaga acccgaagcg cgccatcatg gtcacgctct cgctcgctgg
      361 cggcgtgcag atgggagccg ccttccccgg actcgacgcc gtgctcctcg gcgcacaccc
      421 gcactggctc acccactcgg cgctgcccgt ctgggccttg cgtaaggccg gcgagaagtt
      481 cgacagttac ctgcaagagc gcgaggagtt ggtggcggcc ggcgaactgg acgaggccga
      541 gcgcaagcgc gtcgagttcg agcgcaagat cgctcgctac gtgggcgcgc cgctcctcgg
      601 cgcgttcagt tgcgccgcgg gcctgacgat gtgggcgcag attctccaac ccggcacggt
      661 cgtcggcgcg cccatcagtt ggctcatcgg cggcaacccc ttgctctcag gcatctggct
      721 tttcgccaac ggcgtcatct gcttccagca aggctacaag ttcttcatga tcgcactccg
      781 cgatcaggag gacgcgcaga gaatcgtcag ggaaataagg ggcttgctgc ctgcggctgc
      841 ttcggcctag aagcgcggtg gcggccgttt tgtcgggaaa tcaagaccgg aagtaaactg
      901 gaagcacact atcgaaggcg gcaaacgaag gcagcgcacg aagaggccac ccacaatggg
      961 cggcctcttc gtgtttctgc ccggcccgga cttatacttg aatgatgagc acaagcgtgg
     1021 cgagtaatga gatggcccgc cgcgaacagg tgcggcgcgg gcgcctgctc gaatacctga
     1081 cggtgggctg gaactctctt gaaggaatca tcgccctcgg cgcgggcttc gccgccggca
     1141 gcatcgcttt ggtcggcttc gggttcgatt cgataattga agtcacgtcg ggcgtcgctc
     1201 tgctctggcg gttacattta gacgctcccg aaaggcgcga gcgggctgag caaatttctc
     1261 tcaaaatcgt cggcgtcagc tttctcgcgc tcgcggccta tgtcgcctat gacgccgtca
     1321 ggtcgttaat cacgcgcgag tcgccggagg caagttatgt cggcatcgct ctggctgcgc
     1381 tctctttagt cgtgatgccg cttttggcgc gggcgaagcg cggtgtggca gcgaagatta
     1441 acagccgcgc catgcaggcc gactcgcggc agacggacat ttgcgcctac ctttccgcca
     1501 tcctgctcgg cgggctgata ctcaacgcgc tcctcggatg gtggtgggcc gaccccgtcg
     1561 ccgcgttggt gatgacgccg attatcgtga aggaaggcgt cgaggcgctg cgcggcgaga
     1621 cctgctgcga cgaaggggcg tgccattgat gctgacgccg acgacgactt acatcctcct
     1681 gctcatcgtg caggcgtttc acctgttaca tcatcgtctg gtgaaacgtc atatcagctt
     1741 cgcagaggtt gtttcggcgg cggtgctttg cgtcccgcca tttgccgtgc cgcttccggc
     1801 gtggcttttt ataggcgcgc atctgtcgtt gatagccatt caaattgtcg ggagtttgtg
     1861 gatcaggaaa ctatcgcctg attggtcaaa gtcggaatag attcgttttt aacccgactc
     1921 agacacttct ggttttagat gtgcgagctc tctgttgaaa gctgcgctcg tgctgttcgg
     1981 taacatcagt ggagttggag aatgatgcga aaaagtattc tggcgttttt actattgtca
     2041 tcgctcgccg gactggcgac ggcgcaaaca ggcgtggtca aactcgaagg tcaggtcgtg
     2101 tgctgcgccg attgttgggc ggaagccgac cgcacgaaag ttgaatacgg cacggcggaa
     2161 gatctgctga aggctcaatc ctgcgtggcg aatggcgacc cgacgttgct tgcggtacga
     2221 gagggcgaca agttcactct ctatcagctt gagcaggcaa agtatcgact gcccggtaag
     2281 aactggctcg atttcgtggg caagcgcatc gccgtgacag gcgcggtgcg gaagaagaaa
     2341 aacgcgggcg tgattcgcgt cgatgctctg gaagtgctcg ccccgagtct cgcggagcag
     2401 gaagccgcga acgtcgtcgg caaggaagtt gatttaatcc tcaaagaccc gtttggcacg
     2461 gagcagcgtt tgagtgcgct caagggccgg attgtcgtct tgaatttctg ggcgacgtat
     2521 tgcgtgccat gccgcaagga aatgcccgac ctcgcggcca ttcaaaacga gtacgccgct
     2581 ctgggggttc aggtagtcgg ggcttcggcc gacgcgccgg aagaccgcgc caaggttctg
     2641 gagttcgtca aagaaacgaa agttaatttc cccgtctgga cgggcgcgag cacggcggat
     2701 atgttgcgct tcggcctcgg cgcggcctta cccggtacgg tcatcgtcgg cagggacggg
     2761 cgcgtggcca aagttatttc cggcatcgtc aatcaggccg atttgaaaaa gcagatagaa
     2821 gccatgctcg cctcggcgga gaagacgggc gcggcgcagc gtcaggcgac gaagcgtgag
     2881 caggcggctt ccgtcaagga gaagcgaagt gaagcgtctt ccgtcccgtc ttgaccgctt
     2941 tgacccggtg agggccg
//
LOCUS       DASVES010000354         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_480394, whole genome shotgun sequence.
ACCESSION   DASVES010000354 DASVES010000000
VERSION     DASVES010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480394"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VF240_14465"
     CDS             complement(<1..1122)
                     /locus_tag="VF240_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX7176465.1"
                     /translation="MLRNTILLAGFLIAATALSLDNLTNAQSQSPSAPAEWLTHAERT
                     DYRETPNYADTIAYARRLDQHSPWVRLTSFGRSGEGRELHLLVAAKGDTFTPEAARKS
                     GKPVVLIQACIHAGETDGKDAGLALLRDIAVTKTHEKLLDRAVVLFIPIYNADGHERR
                     SPYNRINQNGPAEMGWRGNASNLNLNRDYMKADAPETRAWLRLWNEWSPDLFIDCHVT
                     DGADFRYTVTYQFEQHENVLPPVRRWSREVFEGRILPAAEAMDSANLFSPYMVFRDNR
                     DPAKGIDSFIATPRFATGYVPVVRNRPALLIETHMLKPHRLRVRGTYDILRAALEEVN
                     KNPDELLRTAREADASVVQGGSRYDPAGKVALSVELTDKP"
     gene            complement(1203..1394)
                     /locus_tag="VF240_14470"
     CDS             complement(1203..1394)
                     /locus_tag="VF240_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176466.1"
                     /translation="MYLSPGFTWGETLLSVAVLVGILGLSAVVTNWFARKMYVRCGPC
                     GALNARRRTHCRVCENPFD"
     gene            complement(1394..2173)
                     /locus_tag="VF240_14475"
     CDS             complement(1394..2173)
                     /locus_tag="VF240_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176467.1"
                     /translation="METVTGIFRSRTDAERAAEQLRTQGIAEDKINLLAPGTSEGQVE
                     AAVPTTETEQPGMGGALGGAVGGALGVAGGLHLGAAAASLFIPGVGPVLAAGIIGAAL
                     LGAGGAAAGAAAGSAAEGAVAGGLPHDEIFVYEDALRQGRTVIIAVVEDDQAEVARVA
                     LSNNGAESIDAAREQWWVGLRDAEEAVYSEEGGDFNVDEGDYRSGFVAALHPLARGKS
                     YDDDQERLRECYGGACSSEAFRRGYARGRGHQSGLEEKFKS"
     gene            2494..2868
                     /locus_tag="VF240_14480"
     CDS             2494..2868
                     /locus_tag="VF240_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176468.1"
                     /translation="MFDIIDKHWPMSVGDGKATRKKKPRLLAITTDCCTGCAGSPACI
                     EYCPIEGCMFWVPDEDHPPFGRIEVDPYLCIGCQKCISKGPDGAFLDGCPWDAIEMIP
                     TDDWEAVHGINLPDAPPPPPTT"
     gene            complement(2876..>3593)
                     /locus_tag="VF240_14485"
     CDS             complement(2876..>3593)
                     /locus_tag="VF240_14485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7176469.1"
                     /translation="DEQIEKYFNEGGNPRHVQHALSQIGEGLEAGKPGVGRWFPRVVE
                     ASGSQSADVRMTAAWVMGLEHRRDDFHEALLRLLADPEPIVRRNAALALVRFGDPSCR
                     PELLAMLLPHTVTARTEGIALTALTAGTPVRRDTLLLRYKSKPNLDDEVRSPLPGKIS
                     QAMVKEGDNFRAGDVLFVIAPDGEHVRDALIGLQFFGEAEDLPEMERFAVGVEGMSEE
                     VKKQAAATAEAVRRRSSGRS"
BASE COUNT          595 a         1171 c         1174 g          653 t
ORIGIN      
        1 cggcttatcc gttaactcaa cgctcagcgc cacctttccg gccgggtcgt agcggctgcc
       61 tccctgaacg accgatgcgt cggcctcgcg ggcggtgcgg agtagttcgt cggggttctt
      121 gttcacctct tcaagcgcgg ctctcaggat gtcgtaggtg ccgcggacgc gcaggcggtg
      181 cggcttgagc atgtgcgtct cgatgaggag cgcggggcgg ttgcggacga cgggcacgta
      241 gccggtggcg aagcggggcg tggcgatgaa actatcgatc ccctttgcgg ggtcgcggtt
      301 gtcgcgaaaa accatgtagg gcgagaaaag gttggcggag tccatcgcct cggcggccgg
      361 gagaatgcgc ccctcgaaga cctcgcgcga ccaacggcgg acgggcggca ggacgttttc
      421 gtgttgctcg aactgatagg tgacggtgta gcggaagtca gcgccgtccg taacgtgaca
      481 gtcgatgaag aggtcgggcg accactcgtt ccagaggcgc agccaggcgc gcgtctcggg
      541 cgcgtcggcc ttcatatagt cgcggttgag attgaggttt gaggcgttgc cgcgccagcc
      601 catctcggcg gggccgttct ggttgatgcg gttgtagggg ctgcggcgct catgcccgtc
      661 agcgttgtag atgggaataa atagcaccac ggcgcggtcg agcaatttct cgtgagtctt
      721 ggtgacggcg atgtcgcgca gtaacgcgag gccggcgtct ttgccgtcgg tctcgccggc
      781 gtggatgcag gcttgaatga ggacgacggg cttcccggac ttgcgcgccg cctcgggcgt
      841 gaaggtgtcg cccttggcgg cgacgagcag atgcagctcg cgtccctcgc cgctccgccc
      901 gaaggaggtc aggcgaaccc acggcgagtg ctggtcgagg cgtcgggcat aggcgatggt
      961 gtcggcgtag ttcggcgtct cgcggtagtc ggtgcgctcg gcgtgtgtca gccactcggc
     1021 cggcgccgag ggcgactgac tctgcgcgtt cgtcaaatta tccagactca aggcggtggc
     1081 ggcgatcaga aagccggcga gcaaaatcgt gttgcgaagc atcaacggtc tccacctgta
     1141 cgggcgttgg agtgggccgc aggcgtgtga aagaagcagg ccggggaagg cgccgccgcc
     1201 gctcagtcga acggattctc gcacacacgg cagtgtgtgc gccgtctcgc gttgagcgcg
     1261 ccacacggcc cgcaacggac gtacatcttg cgcgcgaacc agttggtgac gacggcgctt
     1321 aagccgagta tgccgacgag gacggcgacg ctcaggagcg tctcgcccca ggtgaaaccg
     1381 ggcgacaggt acatcaactc ttaaacttct cctccagccc cgactggtga ccccggccgc
     1441 gcgcgtagcc gcgccgaaag gcttctgatg agcaggcgcc tccgtaacac tcgcgcagtc
     1501 gttcctgatc gtcgtcgtaa ctcttgcccc gcgcaagcgg atggagcgcc gccacgaagc
     1561 cgctccggta gtcgccttcg tccacgttga agtcgccgcc ctcttccgag tagacggcct
     1621 cctcggcgtc gcgcaggccg acccaccact gctcgcgcgc cgcgtcgatg ctctcggctc
     1681 cgttgttgga gagcgcgacg cgcgccacct cggcctgatc gtcttcgacc acggcaatga
     1741 ttaccgtgcg gccctgccgt agcgcgtcct cgtagacaaa aatctcgtcg tgcggcagac
     1801 cgccggccac ggcgccctcg gccgctgacc cggcggccgc gccggccgcg gccccacccg
     1861 cgccgagcag cgccgcgccg atgattccgg cggcgaggac ggggccgacg cccggtatga
     1921 agaggctggc cgctgccgcg ccgaggtgca atccgccggc cacgccgagc gcgccgccga
     1981 cggctccccc aagcgccccg cccatgccgg gctgctcggt ttcggtcgtg ggcacggcgg
     2041 cctcgacctg tccctccgaa gtgcccggag cgagaaggtt aatcttgtcc tccgcgatgc
     2101 cttgcgtgcg tagttgttcg gcggcgcgct cggcgtccgt gcgcgagcgg aatattccgg
     2161 tgaccgtctc catgcgtgtt caaggtttgg gcgcagcgaa tgtgtgcgtg cgtccgggtc
     2221 gcctttcccc gacctctgtg tggacaccta cttgtccgta tcaatcaaga cttgtttggc
     2281 tggaaagatt ctatcacgaa gactccgcgt aataacgcga acacgcggga agaagtgcgt
     2341 gtatggcgca ggctccgtcc gggtcataat cccgcttgag ggcaacgttc gcctccatac
     2401 ctgacgccgg aaaattgatt gtggggagtg cgcgcgccgt gttaaagttg gcgtgtcttc
     2461 ttgaactccc cgctgttatt tctggagtta taaatgttcg acatcatcga caagcactgg
     2521 ccgatgtccg tcggcgacgg caaggccacg cgaaagaaga agccgcgcct gctggccatc
     2581 accaccgact gctgcacggg ctgcgccggc tcacccgcct gtatcgaata ctgccccatc
     2641 gagggctgca tgttttgggt gccggacgaa gaccatccgc ccttcggccg catcgaggtt
     2701 gacccatatc tctgcatcgg ctgccagaag tgcatcagca aaggccccga cggcgccttc
     2761 ctcgacggct gcccatggga cgccatcgag atgatcccaa ccgacgactg ggaggccgtg
     2821 cacggcatca acctgcccga cgcgccgccc ccgcccccga cgacttaaag caccatcaac
     2881 ttcttccaga cgaacggcgg cggacggctt cggcagtcgc cgccgcttgt ttcttcacct
     2941 cttccgacat tccctcaacc cccacggcga aacgttccat ctctggcaag tcttccgcct
     3001 cgccaaaaaa ttggagaccg atgagcgcgt cgcggacgtg ctcgccgtcg ggcgcgatga
     3061 cgaagagaac atctccggct ctgaagttgt cgccctcctt caccatcgct tgcgaaatct
     3121 ttccgggcaa tggagaacgc acttcatcat ccagattcgg cttgctcttg tacctgagca
     3181 acaaggtatc tctgcggacg ggtgtgccgg ccgtgagggc cgtcagggca atgccttccg
     3241 tccgcgctgt cacggtgtgc gggaggagca tcgccagaag ctcggggcgg caactcggat
     3301 caccgaagcg cacgagggcg agcgcggcgt tgcgcctgac aatcggctcg gggtcagcga
     3361 ggagtcgcag cagcgcctcg tggaagtcgt cccggcggtg ttccagcccc atcacccacg
     3421 ccgccgtcat gcgaacgtcc gccgactgac tgcccgaggc ttcgacaacg cgcgggaacc
     3481 agcgcccgac gcccggcttc cccgcctcca acccttcacc tatctgcgac agtgcgtgct
     3541 ggacgtggcg cgggtttcca ccctcgttga aatacttctc gatctgctcg tcg
//
LOCUS       DASVES010000355         6986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_109512, whole genome shotgun sequence.
ACCESSION   DASVES010000355 DASVES010000000
VERSION     DASVES010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109512"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VF240_14490"
     CDS             complement(<1..452)
                     /locus_tag="VF240_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7176470.1"
                     /translation="MNKLRILLAEDHETIRDGLKLLLNAQPDMEVVGEAANGRVAVQL
                     AQQLLPDVVVMDVSMPELNGLQATKKLKETCPQVKVLTLTRHTDDGYLQQLLQAGVSG
                     YVLKQSKSVELLRAIRAVVAGQAYLDPAITERAVTQIRDGRKAASGFSA"
     gene            complement(445..3807)
                     /locus_tag="VF240_14495"
     CDS             complement(445..3807)
                     /locus_tag="VF240_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7176471.1"
                     /translation="MSDSSEQNSQPPAGPSERAASFPVIGLGASAGGLQALKDFFTNM
                     PVESGMAFVVIMHLSPKHESHAASLLQATTGMPVTQVTEAVKVEPNHVYVIPPTKNLE
                     MSDGHINLTEPERLHGGKQVAIDLFFRTLGDTHKEHAVGIVLSGMGSDGTNGLKRIKE
                     QNGLAFAQDPKDAEHDSMPRHAIGSGLVDFVLPVAEMPGKLIEVWRNASRIRLPPATE
                     PEVVVSLEDADEAALNDIFLLLRSHTGHDFTHYKRATVLRRIERRLQVNALLELTAYR
                     DFMREHPVEAQALLKDLLISVTNFFRDRAAFDELEREVVPLLFHEKTTGDQVRVWVAG
                     CATGEEAYSVAMLLLEYAERLPSPPAIQIFATDIDEAAIATAREGSYTETIEADVSME
                     RLRRFFTKESGGYRIRKDVRERVLFAVHNLIKDPPFSRLDAVTCRNLLIYLNREAQTH
                     IFELFHFVLLPNGFLFLGNSESADGVANLFTTVDKRERIFRAESVTRARLRVPSLPLS
                     LPPPRGSAPQAQGARAQQQITFAELHQQLLEQYAPPSVIIDERNDIVHLSDRAGRFLQ
                     FVGGEPSHNLLKVVHPELRIELRTALFQAAQTGKSCVARRVYITRDGRDLYVNMIARP
                     VGGDAPSPNYFLVLFDEVEGLTGARSDVANAGDETEPVVRLLEEELHTSKEQHQVTVE
                     QYETQAEELKASNEEMQSMNEELRSATEELETGKEELQSVNEEIQTINSELKSNLAEL
                     AIANSDLKNLIASTDLATIFVDPNLNIKFYTPRAQDIFSLIPSDIERPLSDITHRLDY
                     ADLLPDVGRVLADSNKREQEVASREGRWYRAQLGPYRGGGERTEGVILTFVDFTTRKL
                     AEIETLQAKESLERQTISLMEANAALQAEINERLRGERERGQLIRHIVFAQEEERRRI
                     AREMHDQFGQQLTVLKIKLDALKEDCGDNENLCEQVTALQAVASQLDADVDYLVWEMR
                     PTALDDLGLQAALSTYVESWSKHFGVPVQLHASGMDRERLTPESETALYRIAQEALNN
                     VAKHAQAASVAVVLERRAAQVSLIVEDDGVGFDLQQVFETGDQGLGLIGMRERAALVG
                     GTVEIESQPNEGATVVVRIPVPSAPPAGETDE"
     gene            4031..4477
                     /locus_tag="VF240_14500"
     CDS             4031..4477
                     /locus_tag="VF240_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7176472.1"
                     /translation="MRRREGDGCPVAGALQMLGDKWTMLIVRDLAGAPRRTTELLGAL
                     HPISSRTLVERLRDMERDQLVERRNLGGSPPHVEYLLTGRGRQLLPLLEALRAAGEAL
                     DCNACDDRFARSGDYCEACPNRHAAPRPAPARPHARSRGDDSIVLL"
     gene            4621..5541
                     /locus_tag="VF240_14505"
     CDS             4621..5541
                     /locus_tag="VF240_14505"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7176473.1"
                     /translation="MKKLLLVLCVFFAPAAFAQKKVDVAAELRSVVQAEREFARTAAA
                     SGMRASFLAFIADDGLLFRPGPVSGKKWWTERPERPGLLSWEPVYADVARAGDIGYTT
                     GPWEFRAGAPADKPAGYGQFMTIWGKQPDGTWKFLLDIGTSNPAPTGLPPSLSFASDF
                     RKNTDKDKLSVNTARVRDELLKLERDFASASAKDTAAAFDAHAAGDIRLMRDGHFPFL
                     GKEAMRKALAAEAGALAWEPAQAVASRSGELGYTYGTYELRRAEASAVERGHYVRIWK
                     KKSDGGKKSDDSWKVVLDLLSPLPPPPSAN"
     gene            5553..6884
                     /locus_tag="VF240_14510"
     CDS             5553..6884
                     /locus_tag="VF240_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7176474.1"
                     /translation="MRNNRQGVGERPQSRASRRAKLLLALAGVVFGLLLAEVALRATG
                     YSFPEFYTTDERRGYALRPGAAGWYRKEGASYVRINSDGLRDREHAREKPTDTFRIAV
                     VGDSYAEALQVELAASFWAVMERRLQACAAQFAGGRRVEVINFGVSGYGTAQELITLR
                     ERVWDYSPDLVLLAFTTNNDITDNSRALKKTDQTPYFVLRDGRLSLDDSFRETRAFRL
                     NRSPLGRLGASLVSGSRVVQLTQQAHAALRAYMKRPRAAAASPPAQQTARARRQTVLL
                     THRSAGSSPPQTQPTTTKDGATPGEELGIDNLVYRAPPDQTWGEAWRVTEELLKTMRD
                     EVRGRGAEFLVVTLSNGIQVYPDPAARAAFMQRVGATDLFYPDARVRALGEREGFPVL
                     NLAPALQEYADRERVFLHGFGRELGNGHWNELGHRVAGEMIAENLCAGAAR"
BASE COUNT         1187 a         2313 c         2189 g         1297 t
ORIGIN      
        1 gcagaaaagc cgctcgcggc ttttctgccg tcacgaatct gagtgacggc cctttcggtg
       61 atcgccgggt cgagatatgc ctgaccggcg acgacggcgc gaatcgcgcg cagcagctcc
      121 acggacttgc tctgcttcag cacgtaaccg ctcacgcccg cctgtagcag ttgttgcagg
      181 tagccgtcgt ccgtgtggcg cgtcagggtc agaaccttta cttgcgggca tgtctccttg
      241 agcttcttcg tcgcctgaag accgttcagc tccggcatgg aaacatccat cacgacgaca
      301 tcgggcagga gctgctgcgc gagctgcacg gcgacgcgcc cgttggcggc ctcgccgacg
      361 acctccatgt cgggctgcgc gttgagcaga agcttcagcc cgtcgcggat tgtttcgtga
      421 tcctcggcta aaaggatgcg gagcttattc atccgtctct cccgccggcg gcgcgctcgg
      481 cacgggaatc cgcacgacca cggtggcccc ctcgtttggc tgcgactcaa tctcgaccgt
      541 gccgccgacg agtgcggcgc gttcgcgcat cccgatcagc cccagtccct gatcgccagt
      601 ctcgaacacc tgctgcaagt cgaaaccgac gccgtcgtct tcgacgatca gggagacttg
      661 ggcggcccgg cgttcgagca cgaccgcgac gctggcggct tgcgcgtgtt tggcgacgtt
      721 gttgagggct tcctgcgcga tgcgatagag cgcggtttcg ctctccggtg ttaagcgctc
      781 tctatccatg ccgctcgcgt gcagttgcac gggaacgccg aaatgttttg accagctctc
      841 gacgtaggtg gagagcgcgg cctgtaaacc gagatcatca agcgccgtcg ggcgcatttc
      901 ccagaccaga taatcaacgt cggcgtcgag ttggctggcg accgcttgta atgcggtcac
      961 ctgttcgcat aggttctcat tatcaccgca gtcttctttg agcgcgtcga gcttaatttt
     1021 gagcacggtc aattgctgcc cgaactgatc gtgcatctcg cgcgcgatgc gccggcgctc
     1081 ctcctcctgt gcgaagacga tgtggcgtat aagctgcccg cgctcacgct cgccgcgcag
     1141 ccgctcgttg atttccgcct gcaaggcggc gttcgcctcc atcagcgaga tggtctggcg
     1201 ctccaaactt tccttcgcct gcaacgtctc gatctccgcc agcttgcgtg tggtgaaatc
     1261 gacgaaggtg agaatcacgc cttccgtgcg ctctccgccg ccgcggtatg ggcccaactg
     1321 cgccctatac cagcgcccct cgcggctcgc cacttcctgc tcccttttat tcgagtcggc
     1381 gagcactcgt cccacgtccg gcaacaggtc ggcgtagtcg agtctgtgcg tgatgtcgga
     1441 gagcggccgc tcgatgtccg aggggatcaa gctgaaaatg tcctgcgcgc gcggcgtgta
     1501 gaatttgata ttcaggttgg ggtcaacgaa gatggtcgcc agatcggttg aggcgatcaa
     1561 atttttcagg tcgctgttgg cgatggccag ctcggcgaga ttactcttca actcgctgtt
     1621 gatggtctgg atttcctcgt tgaccgattg caactcttcc ttgcccgttt ccagctcctc
     1681 ggtggccgaa cgaagctcct cgttcatcga ctgcatctct tcgttcgagg ctttcagctc
     1741 ttcggcctgc gtctcgtact gctcgacggt cacctgatgc tgctctttcg acgtgtgcaa
     1801 ctcctcttcc aggaggcgca cgaccggctc agtttcgtcg cccgcgttcg ccacgtcgct
     1861 tctcgcgccc gtcagccctt cgacttcgtc aaagaggacg agaaagtaat tcggcgacgg
     1921 cgcgtcgccg ccgacgggac gggcgatcat gttgacgtaa agatcgcgcc cgtcgcgggt
     1981 gatgtatacg cgccgtgcta cacatgattt gccggtctgc gccgcttgga acagggccgt
     2041 gcgaagctca atccgaagct ccgggtgtac gaccttcagg aggttatgac tcggctcgcc
     2101 gcccacgaat tgcagaaatc tgccggcgcg gtcggacagg tggacgatgt cattgcgctc
     2161 gtcgatgatg acgctcggcg gcgcgtattg ctccaaaagt tgttggtgca gctcggcgaa
     2221 cgtaatctgt tgctgcgcgc gcgccccttg cgcctgcggc gcggagccgc gcgggggcgg
     2281 caggctgagc ggcaggctcg gcacgcgcag ccttgcgcgg gtgacgcttt cggcgcgaaa
     2341 gatgcgctct cttttatcca cggtcgtaaa caggttcgcc acgccgtcgg ccgattccga
     2401 attgcccaga aacagaaacc cgttggggag cagcacgaag tggaacaact cgaagatgtg
     2461 cgtctgcgct tcgcggttga gatagatcag caggttgcga caggtgacgg cgtcgaggcg
     2521 cgagaacggc gggtctttaa tcagattgtg gacggcgaac agcacgcgct cgcgcacgtc
     2581 cttcctgatg cggtaaccgc ccgactcttt cgtgaagaag cgccgcagac gttccatcga
     2641 cacgtccgcc tcgatggttt ccgtgtagct gccctcgcgc gccgtggcaa tcgccgcctc
     2701 gtcgatgtca gtggcgaaga tttgaatggc gggaggagac gggagccgct ccgcatattc
     2761 caacagcagc atcgccaccg agtacgcctc ttcacccgtc gcgcaaccgg ccacccacac
     2821 gcgcacctga tcgcccgtcg tcttttcgtg aaaaagcagg ggcacgacct cgcgctccag
     2881 ctcgtcgaaa gccgcgcggt cacgaaagaa gttcgtcaca ctgatgagca aatctttcaa
     2941 taacgcctgc gcttctacgg gatgctcgcg catgaagtcg cggtaagccg tgagttccag
     3001 caaagcgttg acctgaaggc gtcgctcgat gcggcgcaac acggtcgcgc gcttatagtg
     3061 cgtgaagtcg tgtcccgtgt gtgagcgcag gagcaaaaag atgtcgttca gggctgcctc
     3121 gtccgcatcc tccagactga cgaccacttc cggctcagtt gccggcggca acctgatgcg
     3181 gctcgcgttt cgccagactt caatgagctt gcccggcatc tccgccaccg gcagcacgaa
     3241 atcaaccaga ccgctgccta tcgcatggcg cggcatcgaa tcgtgttcgg cgtctttcgg
     3301 gtcttgcgcg aacgccagcc cgttctgctc cttgatgcgt ttgaggccgt tagtgccgtc
     3361 cgaacccatc cccgacaaca cgatcccgac ggcgtgctcc ttatgcgtat cgccgagcgt
     3421 gcgaaagaaa agatcgatgg cgacctgctt ccccccgtgc aggcgttccg gttcggtgag
     3481 gttgatgtgc ccgtcgctca tctcaaggtt cttcgtcggc gggatgacgt agacgtggtt
     3541 cggctcgacc ttgacggctt cggtgacctg tgtgaccggc atcccggtcg tcgcctgcaa
     3601 cagcgaggcg gcgtggctct cgtgcttcgg cgacaggtgc atgatgacga cgaaggccat
     3661 cccgctctcg acgggcatgt tcgtgaagaa atcctttaac gcctgcaatc cacccgccga
     3721 cgcgcccagc ccgatgacgg gaaaagacgc tgcccgttcc gacggccccg ccggtggctg
     3781 agaattttgc tccgaactgt cgctcataag gttcacggcc cttccctttg gccacgaagt
     3841 atcacacgaa acaattaata ggtcacgagc gagactgccc cgcggcccct gttttgtgtc
     3901 agccgccgcc cggtttgtgc taagcttcgc gccggccgcg cgaaagattg atgggccggc
     3961 gacgccgggg cacgcccgcg gaggtttgtg ataagtgcgt cgggcgcggc gggtggggga
     4021 agagccgaaa atgcgacgac gcgaaggcga cgggtgcccg gtggcgggcg cgctccagat
     4081 gctcggcgac aagtggacga tgctcatcgt ccgcgacctc gcgggcgcgc cgcgccgcac
     4141 cacggaactg ttgggcgcgc ttcaccccat cagcagccgc acgctcgtcg agcgcctgcg
     4201 cgacatggag cgcgaccagc tcgtcgagcg gcgcaacctc ggcggcagcc cgccgcacgt
     4261 cgagtacctg ctcacgggtc gcggtcgtca gctcctgccg ctcctcgaag ccctgcgcgc
     4321 ggcgggcgag gcgctcgact gcaacgcctg cgacgaccgc ttcgcccgct ccggcgacta
     4381 ctgcgaagcc tgccccaacc gccacgccgc gcccaggccc gctccagcgc gcccacacgc
     4441 gcgcagccgc ggcgacgatt ccatcgtctt gttgtaattg agttcaaagt tcaaagttca
     4501 aagttcaaag gccgtacagc ttttgacttt gaactttgaa ctttgaactt tgaactaatg
     4561 ttgactttga actttgaact ttgaactttg aacttcatag ttgtgttcgg gagttagcgg
     4621 atgaagaaac tgcttctcgt cctgtgtgtg ttcttcgcgc ccgcggcctt cgcgcagaaa
     4681 aaggttgacg tggcggcgga gctgcgttcg gtcgtgcagg ccgagcggga gtttgcgagg
     4741 acggcggccg cgagtgggat gcgcgcgtct ttcctcgcct tcatcgccga cgacggcctg
     4801 ctcttccggc ccggccccgt gagcggcaaa aagtggtgga cggagcggcc cgagcggccc
     4861 ggtctgctct cgtgggagcc ggtctacgcc gacgtagcgc gcgccggcga catcggttac
     4921 accacggggc cgtgggagtt tcgggcgggc gctccggcag acaagcccgc cggctacggc
     4981 cagttcatga ccatctgggg caagcagccc gacggcacat ggaagttcct gctcgacatc
     5041 ggcacgtcga acccggcgcc cacgggcctg ccgccctcac tctccttcgc ttcggacttc
     5101 aggaagaaca cggacaagga caagctgagc gtcaacaccg cgcgagtgcg cgacgagctg
     5161 ttgaagctgg agcgcgactt cgcgtcggcc tccgcgaagg acacggccgc ggccttcgac
     5221 gcgcacgccg ccggtgacat acgcctgatg cgcgacggcc acttcccctt cctcggcaaa
     5281 gaggcgatgc gtaaggcgct cgccgccgaa gcgggcgcgc ttgcgtggga gccggcgcag
     5341 gccgtcgcgt cgcgctcagg cgaactcggc tacacctacg gcacatacga actgagacgc
     5401 gctgaggcgt cggcggtcga gcgcggtcac tacgtgcgta tctggaagaa gaagtcggac
     5461 ggcgggaaga agtcggacga cagttggaag gtcgtgctcg atctgctcag cccgctcccg
     5521 ccgccgccga gcgcgaattg aagaccgccc cgatgcgaaa caaccgtcag ggggtgggtg
     5581 agcgtcctca gtcccgcgcc tcgcgacgcg ccaaactatt gcttgcgctc gccggcgtcg
     5641 tcttcggatt gctgctcgcc gaggtcgccc tgcgcgcgac cggctactcc ttcccggagt
     5701 tctacacgac cgacgagcgg cgcggctatg cgctccggcc cggcgcggcc gggtggtatc
     5761 gcaaagaggg cgcctcttac gtccgcatca actcggacgg cctgcgcgac cgcgagcacg
     5821 cgcgcgagaa accaacagac accttccgca tcgccgtcgt cggcgactcc tacgccgagg
     5881 ccctgcaggt cgagctggcg gcctccttct gggccgtcat ggagcgccgc ctgcaagcgt
     5941 gcgcggcgca gttcgccggc gggcgtcgcg tcgaggtcat caacttcggc gtctccggct
     6001 acggcacggc gcaggagttg atcacgctgc gcgagcgcgt ctgggactac tcgcccgacc
     6061 tcgtccttct ggccttcacc accaataacg acatcaccga caactcgcgc gccctcaaga
     6121 agaccgacca gacgccctac ttcgtgctcc gcgacggccg gctctccctc gatgattcct
     6181 tccgcgagac gcgcgccttc cgcctcaacc gctcgcccct cggtcggctc ggcgcatcgc
     6241 tcgtctccgg ctcgcgcgtc gtccagctca cgcagcaggc gcacgccgcg ctgcgcgcct
     6301 acatgaagag accacgcgcc gccgccgcgt cgccacccgc gcagcagacc gcgcgcgccc
     6361 gacggcagac agtgcttctg acacaccgca gcgccgggtc gtcgccgccg caaacgcagc
     6421 cgacgacaac aaaggacggc gcgacgcccg gcgaggagtt gggaatcgac aacctcgtct
     6481 accgcgcgcc gcccgaccag acgtggggcg aggcgtggcg cgtcacggag gagttgctca
     6541 aaacgatgcg cgatgaagtc cgcgggcgcg gcgcggagtt tctcgtcgtc acgctctcaa
     6601 acggcataca ggtctacccg gacccggccg cccgcgccgc cttcatgcaa agggtcggcg
     6661 ccacagacct cttctacccg gacgcgcgcg tccgcgccct cggcgagcgc gaaggcttcc
     6721 ccgtcctcaa tctcgcgccc gcgcttcagg agtacgccga ccgcgagcga gtctttctcc
     6781 acggcttcgg ccgggaactc ggcaacggcc actggaacga actcggccac cgcgtcgcgg
     6841 gcgagatgat tgccgaaaac ctctgcgcag gcgcggcgcg ttgaagatgt ttaatgagtc
     6901 gcggagcgac ggaagaatgt agcccacggc gtcgggccgt gggtcagcgt cgcttaaaaa
     6961 cacggaagcc ccgtcagggg cgaaag
//
LOCUS       DASVES010000356         6115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1016304, whole genome shotgun sequence.
ACCESSION   DASVES010000356 DASVES010000000
VERSION     DASVES010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016304"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1056
                     /locus_tag="VF240_14515"
     CDS             175..1056
                     /locus_tag="VF240_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176475.1"
                     /translation="MSSTVETREKRVAGGGEAANLNWSLWARQTLAVLRLELGRNFLG
                     RRALLLYVLAGLPLSVLAAVVVFLPSAGGETEGFGELGTAYSVIYGALILRTLLFFGC
                     AWVFMNLFRGEVVDRSLHYYFLAPVRREVLVVGKYLSGLVATVTLFGVSTLGSFALLY
                     FGLFPSQSMEYVFNGPGFAQALTYAGITTLACVGYGAVFLIVGLFFRNPIIPAGLIYG
                     WEWINFLLPPLLKKVSVIHYLHSLVPVPPPQGPFAVLAEPTPAWISVPSLLIFTALTL
                     VLAGLHIRRQEISYAGE"
     gene            complement(1107..1928)
                     /locus_tag="VF240_14520"
     CDS             complement(1107..1928)
                     /locus_tag="VF240_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7176476.1"
                     /translation="MAERMRLEDRTAVITGAAGGIGRAIAVSLARRGCHLALADVDEA
                     GMAGTEELVLAHGVRVTRQRLDVADRAAVAGFPARVAAEHPGVDLLVNNAGVAAGGTF
                     EQVSEEDFEWLFEINFWGVVRMTRAFLPALRASGDARVVNLSSVFGLVAPPEQVAYAA
                     SKFAVRGFSEALRHELEGSGVGVTVVHPGGVATSISEQARVPAGVSAEEVERRREQFR
                     KLLRLPPEVAGETIVRGIERRQARVLVGSDAKLISVIARILPVSYWKVLALRAGK"
     gene            complement(1921..3438)
                     /locus_tag="VF240_14525"
     CDS             complement(1921..3438)
                     /locus_tag="VF240_14525"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7176477.1"
                     /translation="MNYFSRVGEPSMEHVDVVVVGAGLSGIGAACHLRMECPAKSFVI
                     LEGRAALGGTWDLFRYPGVRSDSDMYTLGYRFRPWQDPRAIADGPAILNYIRETAEEF
                     GVDKKIRYDHRVRRASWSSADALWTVEAEHVNTVVRLTCNFLYLCTGYYDYEGGYTPE
                     WPGVEQFGGLIVHPQKWPEDLDYEGKRVVVIGSGATAVTLVPAMAERAGHVTMLQRSP
                     TYVISRPAQDVVANWLRRRLSARAAYALTRWKNVLLGMFFYRLARSRPAVFKRMLAKG
                     VRRHLGEEYDLKHFTPQYNPWDQRLCLVPDSDLFRAIRDGRASVVTEHIETFTETGLH
                     LKSGEHLDADIVVTATGLVLKLMSGLQLVVDGAPVDLSKTMAYKGMMYSDVPNLASAF
                     GYTNASWTLKCDLTAEYVCRLLKHMDRHGYAQCTPHVNDTEVTEEPILDFTSSYVRRA
                     LHTLPRQGSKTPWRLHQNYVRDLSMLRYGRVDDGAMEFKAALGERRAGGGAAGHG"
     gene            complement(3687..4322)
                     /locus_tag="VF240_14530"
     CDS             complement(3687..4322)
                     /locus_tag="VF240_14530"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7176478.1"
                     /translation="MIESNSAGVGAVILAAGSSSRMGSPKQTLQYRGKSLLRRAALAA
                     LGAGCHPVIVVTGAYAELSRRELEGLGVREVLNTGWETGMASSVRAGVEGLVNADADA
                     DAVVLMLCDQPHVTADVISGLVAAHRAIGSSVVASTYGGSFGVPALFARALFAELTRL
                     EGRSGAKEVIKRHASETHFLPFPSGEVDVDTPDDFSRLSNPTALNNKEYKN"
     gene            complement(4356..5570)
                     /locus_tag="VF240_14535"
     CDS             complement(4356..5570)
                     /locus_tag="VF240_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX7176479.1"
                     /translation="MNEVRDLVEAFDVANARSERCALATVVSVEGSSYRRPGARMLVC
                     EGGGSTGTISAGCLESDVIEHAKRVIRAAVAKLVEYDTASTSDEMAWGLGLGCNGIVR
                     VLVEPLALRSPYVEALRRSCEAHASASPMTVATVYQHTPSGPASAAAHVAIGARLFID
                     EDGEAGRENLSDEVASLIEGDVRQSARAESAGVRVYEVAGGSLKVLVETLLPPVPLIV
                     FGAGHDALPVVELARELGWQTEVVDPQARPASRSRFAMADRVTLARPEEVGAQVSITP
                     RTLTLLMSHNYSHDVTLLRFLLSSPARYIGVMGPRKRTERMLSELAANEETSFLEEAD
                     LTRLYSPAGLDIGANAPAEIALSIVAEMRAALDGRRGGMLREHRGSIHSRQGNGERVP
                     LAEEQALEVAVA"
     gene            complement(5583..>6115)
                     /locus_tag="VF240_14540"
     CDS             complement(5583..>6115)
                     /locus_tag="VF240_14540"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7176480.1"
                     /translation="RFVSVSEVMKRNGLAEIIETFEARPSKEREKYATLAHGAQFVEV
                     KIDPDLGNVRVTRVIEVTACGKIINPKASHSQEIGGVVWGIGMALQEATEIDHRYGRI
                     MNPNLQHYHVPVNADVHEVETMFVEEDDRVVNPLGVKGMGELGMVGIPAAIANAVFHA
                     TGRRLRELPITPDKLL"
BASE COUNT         1017 a         2052 c         1895 g         1151 t
ORIGIN      
        1 ccacaacgac cggcacgggc tgttcgtcaa gacgcgccgg ccagatgact tctacctcct
       61 gctcaaccgg ctcgtgacgg agaatcatct tcaggtcgaa tcggtcgccc cggcggacga
      121 tgacttgaat gccgtttacc aatacttgat cagttccgaa ggagggcgtg taagttgagt
      181 tctacggttg agacgaggga gaagcgtgtg gcggggggcg gcgaggcggc gaatcttaac
      241 tggtcgctct gggcgcggca gacgctcgcc gtcctgcgcc tcgaactcgg aaggaatttc
      301 ctcggccgcc gcgcgctgct gctctacgtg ctcgcggggc tgccgctgtc ggtgctcgcg
      361 gcggtcgtcg tcttcctgcc gtccgcgggg ggggagactg agggcttcgg ggagttgggc
      421 accgcctact ccgtcatcta cggcgcgctg atcctgcgca cgctcctttt cttcggctgc
      481 gcgtgggtct tcatgaattt gtttcgcggc gaggtcgttg accggagctt gcactactac
      541 tttctcgcgc ccgtgcgacg cgaagtgctc gtcgtcggga aatacttgtc ggggctggtg
      601 gcgaccgtca cgctgttcgg tgtctcgacg ctcggctcgt tcgccctgct ctacttcggt
      661 ctcttcccat cacagagcat ggagtatgtt ttcaacggcc ccggtttcgc gcaggcactc
      721 acgtatgctg gcatcacgac gctcgcgtgc gtcggttacg gagcagtttt tctgatcgtc
      781 ggtctgttct tcagaaaccc gatcatcccg gctgggttga tttacggctg ggagtggatc
      841 aactttctgc tgccgccgtt actgaagaag gtgagtgtca ttcattacct tcactcgctc
      901 gtgcccgtcc ccccgccgca agggcctttc gccgtgctcg ccgagccgac gcccgcctgg
      961 atttccgtcc ccagcctgct catcttcacc gcgctgacac tcgtcctcgc cggccttcat
     1021 atccgccgac aagaaatcag ctacgcgggt gaatagacgt gaatctaaag tgcgccgtaa
     1081 gctgccacaa cttccgaact ggagcatcat ttacctgccc tcagcgctaa caccttccag
     1141 taggacaccg gcaggatgcg cgcgatcacc gagatgagct tggcgtcgga gcctacgagg
     1201 actcgcgcct gtcggcgctc aatgccgcgc actatcgtct cgccggcgac ctccgggggc
     1261 agccttaata acttccggaa ctgctcgcgg cggcgctcca cctcctcggc gctcacgccc
     1321 gccgggacgc gggcctgctc cgagattgaa gtggcgacgc cgccggggtg tacgaccgtg
     1381 acgccgacgc ccgagccttc cagctcgtga cgcagcgcct cggagaatcc gcgcaccgcg
     1441 aacttgctcg ccgcgtaggc cacctgctcc gggggcgcga cgagtccgaa cacgcttgag
     1501 aggttcacca cgcgcgcgtc gccgctcgcg cgcaacgcgg gcaggaaggc gcgcgtcatg
     1561 cgtaccacgc cccagaagtt gatctcgaac agccactcga aatcttcctc ggagacctgc
     1621 tcgaacgtgc cgcccgcggc gacgcccgcg ttgttgacga gcagatcgac accggggtgc
     1681 tccgcggcga ctcgcgccgg gaagcctgcg acggcggccc ggtcggctac gtcgaggcgt
     1741 tggcgagtca cgcggacgcc gtgcgctagc acgagttcct ccgtaccggc catccccgct
     1801 tcgtctacgt cggccaacgc gaggtggcaa ccccgacgcg ccagcgacac ggcaatcgcc
     1861 cgcccgatgc cgcccgccgc accagtgatt accgccgtcc ggtcttcgag gcgcatgcgc
     1921 tcagccatgc ccggccgccc ccccaccggc gcggcgctcg cccagagctg ccttgaactc
     1981 catcgccccg tcgtccacac gcccgtaccg aagcatcgag aggtcgcgca cgtaattctg
     2041 gtgcaggcgc cagggcgtct tcgatccctg ccgcggcagc gtgtggagcg ccctccgcac
     2101 gtagctggag gtgaagtcga ggatcggctc ctccgtgacc tcggtgtcgt ttacgtgcgg
     2161 cgtacactgc gcgtagccgt gccggtccat gtgcttcagg agccggcaga cgtactcggc
     2221 cgtgaggtcg cacttcaacg tccaggaggc gttcgtatag ccgaacgcgg aggcgagatt
     2281 tgggacatcg ctgtacatca tgcccttgta ggccatcgtc ttcgacaggt ctacgggcgc
     2341 gccgtccacc acgagttgca gcccgctcat gagctttagc acgagccccg tggccgtcac
     2401 gacgatgtcc gcgtcgaggt gctcgccgga cttgagatgt aggcccgtct ccgtgaaggt
     2461 ctcgatgtgt tcggtgacca ccgaagcccg gccgtcgcgt atcgcccgga agaggtctga
     2521 gtccgggacg aggcacagcc gctgatccca cgggttatat tgcggcgtga agtgcttgag
     2581 gtcatattcc tcgccgaggt gacgcctcac ccccttcgcc agcatcctct taaagacagc
     2641 gggccgcgac cgggccagac gatagaagaa catccccagc agcacgttct tccagcgcgt
     2701 gagcgcgtag gccgcgcggg cgctcaggcg tcggcggagc cagttggcca ccacgtcctg
     2761 tgcgggccgt gagatgacgt aggtggggga gcgctggagc atggtgacgt ggccggcgcg
     2821 ctcggccatc gcggggacga gtgtgacggc ggtggcgccg ctgcctatca ccaccacccg
     2881 cttgccctcg tagtcgaggt cttccggcca cttctgcggg tggacgatca gcccgccgaa
     2941 ctgctcgacg cccggccact ccggggtgta cccgccctcg tagtcgtagt agccggtgca
     3001 gagataaagg aagttgcacg tgagccggac gaccgtattt acatgctcgg cctccacagt
     3061 ccagagcgcg tctgctgacg accaggaggc gcgtctcacc cggtggtcgt agcggatctt
     3121 cttgtccacg ccgaactcct cggccgtctc gcgaatgtag ttgaggatgg caggcccgtc
     3181 ggcgatggcc ctggggtcct gccaggggcg gaacctgtag ccgagcgtgt acatgtcgga
     3241 gtccgaccga acgcccggat agcggaacag gtcccaggtg ccccccagcg cggcgcgccc
     3301 ctcgaggatt acaaaactct tcgcagggca ctccatcctc aggtggcagg ccgcgccgat
     3361 gcccgacagc cctgccccca ccacaaccac gtccacgtgc tccatgctcg gctccccaac
     3421 ccggctaaag taattcatgt ggcgcgcgtg tcagcgcagc actcccgcaa gaattggtat
     3481 ccgacgttac cagcagccat tggtactatc aagtatcagg ccgggttggg tggccgggag
     3541 actttggtgt gatgcctatc gagaggtgag tagtaggtga tgagtgataa gccactaaac
     3601 tttttcgttt ggcgaaggct gttgcgcgtt agcacaaccc gacacaagac agattgtaat
     3661 tatcgataag aatagaaatg cttctttcag ttcttgtact ccttattgtt aagcgctgtg
     3721 ggatttgata ggcgcgagaa atcatccgga gtatccacgt ccacctcacc gctcgggaag
     3781 ggtaagaagt gcgtctccga agcgtgtctc ttgatgactt ctttcgcccc ggacctgccc
     3841 tcgagtcgcg ttagctcggc aaagagcgct ctggcgaaaa gggccggcac gccaaaactt
     3901 ccgccgtagg ttgaggcgac gactgagctt ccgatggcgc ggtgcgccgc gacgaggccg
     3961 gagatgacat cggccgtgac gtggggttgg tcacacaaca tcaagacgac tgcatccgca
     4021 tcggcgtccg cgttgaccag cccctcgacg cccgcccgga ctgacgacgc catcccggtc
     4081 tcccatccgg tattcaacac ctctcgcacg ccaagccctt ccagctcccg tctcgacagc
     4141 tcggcgtatg cgcccgtcac cacgattacg ggatggcacc ccgcgcccag cgcggccagc
     4201 gccgcgcgcc tgagcaggct cttaccccga tattgaagcg tctgtttcgg gctgcccatg
     4261 cgggacgatg accccgccgc cagaatcacc gctccgacgc cggccgagtt gctttcgatc
     4321 actgtgggaa tgctctccgc gtcggccttc gcgcctcacg ccaccgccac ttcaagggcc
     4381 tgctcctcgg ccagggggac gcgctcgccg ttaccctgtc tgctgtggat agagccacgg
     4441 tgctcgcgca acattccgcc gcgccgccca tccagcgccg cgcgcatctc ggcgacgatt
     4501 gacagagcga tttcggcggg agcgttcgcc ccgatgtcca gaccggccgg ggaataaagg
     4561 cgtgtcaggt ccgcttcttc aaggaaggat gtttcttcgt tcgccgccaa ttcactcagc
     4621 atccgttcgg tgcgtttgcg agggcccatg actccgatgt aacgcgccgg ggaggacaac
     4681 aggaacctga gcaacgtcac gtcatgcgag tagttgtgcg acatcagaag cgtcagtgtc
     4741 cgcggcgtaa tgctgacctg cgcgccgacc tcttcggggc gcgcgagtgt gaccctgtca
     4801 gccatcgcaa accgtgagcg gctggcggga cgggcttggg gatcgacgac ctcagtctgc
     4861 catcccaatt cacgcgcgag ttcgacgacc ggcagcgcgt catgccccgc cccaaagacg
     4921 atgagcggga cggggggcag gagcgtttct actaagacct tcaaggagcc gcccgctacc
     4981 tcgtagactc tgacgccggc cgactccgcg cgcgccgact ggcgcacgtc gccttcgatg
     5041 agcgacgcca cctcgtcgct caagttctcg cggccggcct cgccgtcttc gtcgatgaag
     5101 agtcgcgcgc cgattgcaac gtgcgcggct gcggaggccg ggccagaagg cgtgtgttga
     5161 tagacggtcg cgaccgtcat gggcgaggcg ctcgcatgtg cctcgcacga acgtctcaga
     5221 gcctcgacgt agggcgagcg tagggcgagc ggctcgacca gcacgcgcac gatgccgttg
     5281 cagccaagcc caagccccca ggccatctcg tcgctcgtcg aggcggtgtc gtactcgacg
     5341 agtttcgcca cggccgccct gatgacgcgc ttcgcgtgct cgatgacgtc gctctctaag
     5401 cacccggcgc tgatcgtgcc ggtactgcct cccccttcgc agaccagcat ccgcgccccg
     5461 ggccgccggt atgaagaccc ttcgacgctc accacagtcg ccagggcgca ccgctcgctt
     5521 ctggcgttcg ccacgtcgaa cgcttcgact aaatcccgca cttcattcat agttcagccc
     5581 ccttacagca gcttgtccgg cgtaatcggc agttctctga gacgcctgcc cgtggcatgg
     5641 aagacggcgt tggcgatggc cgccgggatg ccgaccatcc ctagctcgcc catgcccttg
     5701 acgccaagcg ggttgactac gcggtcgtcc tcctccacga acatcgtctc gacctcgtgc
     5761 acgtcggcgt tgaccggcac gtggtagtgc tggaggttcg ggttcatgat gcggccgtag
     5821 cggtgatcga tctcggtcgc ctcctgaagc gccatcccga tgccccagac cacgccgccg
     5881 atctcctgac tgtgcgacgc cttggggttg atgatcttgc cgcaggccgt cacctcgatg
     5941 acgcgcgtga cgcggacgtt cccgagatcg gggtctatct tgacctcgac aaactgcgcc
     6001 ccatgcgcca gcgtcgcgta cttctcgcgc tctttagagg ggcgcgcctc gaaagtctcg
     6061 atgatctcgg cgaggccgtt gcgtttcatg acttcggaga cgctgacgaa acgcg
//
LOCUS       DASVES010000357         7696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_256788, whole genome shotgun sequence.
ACCESSION   DASVES010000357 DASVES010000000
VERSION     DASVES010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256788"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1616)
                     /locus_tag="VF240_14545"
     CDS             complement(27..1616)
                     /locus_tag="VF240_14545"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX7176481.1"
                     /translation="MEHVKDDAEPPPQQQARESAMNRFLSFIERAGNVLPHPATLFVI
                     LAAAVIIISAIAAAAGVSVTHPGTKEVIRSESLLTVAGLHRILTGLVTNFTGFAPLGV
                     VLVAMLGIAVAEGSGLISAVLRSLVLSAPRKLLTFVVVLAGVLTHVASDVGYVLLIPL
                     GAMIFLAVGRHPIAGLAAAFAGVSGGFSANFLLGPTDPLLAGFTQEAARIIHPGYVVT
                     PAANYYFMAASAIMITISGTYITEKVIVPRLGEYKGDEKPEELKELTAAERRGLKFAL
                     VVALLFVAFVLAGLVPASGYLRNLKTGGVLDSPFLTGVVAFIFFAGALIGLAYGIGAR
                     TIKSDGDMMKAMEKAMSSMSLYLVLAFFAAQFVAFFNWTHLGVITAVKGAEFLKSSGI
                     SGYPIPMIIAFVCITVLLDLILGSASAKWAVMAPVFVPMFMLVGYSPELTQAAYRVGD
                     SVANIITPLMSYFPLVVAFAQRYDKRAGIGTVMATMLPYSVTFFILWTLLLIFWYLMG
                     LPLGPGAGIFYQPAAPPPPAG"
     gene            complement(1771..2157)
                     /locus_tag="VF240_14550"
     CDS             complement(1771..2157)
                     /locus_tag="VF240_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX7176482.1"
                     /translation="MRFLTLKELIELHRRLIEQSGGSPGIRDLGLLESAMAQPLMTFG
                     GEDLYPTVVEKASALGFSLIKNHPFVDGNKRAGHAAMEVFLVLNGHEIDAGVDDQEGV
                     ILRLAAVEMGREEFTDWLRAHVAEKK"
     gene            complement(2157..2354)
                     /locus_tag="VF240_14555"
     CDS             complement(2157..2354)
                     /locus_tag="VF240_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176483.1"
                     /translation="MSSITIDLSPERLLELQKLAARLGVRPEELARLSIEELLARPDE
                     AFEDAADYVLGKNEELYRRLA"
     gene            complement(2403..4214)
                     /locus_tag="VF240_14560"
     CDS             complement(2403..4214)
                     /locus_tag="VF240_14560"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX7176484.1"
                     /translation="MDEKAGKQTGAPLPVAAPRANGNGNGNGHAKESLSLVPSGNGGA
                     ADDAGAKAVAVSRVGGGVRVTPPSGDAAVATPEDEFALRARGWRGVWRTLQIARVLGV
                     LALFLFLDGYDARRKFNRRVAARLREEARARGRVALFQEWSRDVDRQAADLLTRAVRH
                     FVFRGARGSEEREARLHGQAAWLRRGLIGLGPTFIKIGQALATRADLLPLPYIKELTT
                     LQDQVPPFPTSEAYARIESELGHKLSELYAEIDAEPVAAASLGQVYRARLHTGEEVAV
                     KVQRPNLRETISFDVAVLERLVRHITTRYPQANENADWEGMLREFRQVIFEEMDYAQE
                     GRNAERFRANFREWRVVHVPFIHWTHTSPRVLTMEFIRGTKVVDIEGLRARRISPVKV
                     NRLLVRTYLKQLLEDGFFHADPHPGNLLVMDDGRVAFFDFGMTGRITPQLQSLMIDAF
                     FHVVERDISGLAQDLLNLNFLKPGVDAARVRPVVEGLFQHYINLKLGDVNFKELTYDL
                     AEVMYDYPFRLPYNFTYIMRALMTLEGIGIVTDPGFSFFDTAKPYAKEFMLRREGKQF
                     RKLFVDRLLGRGGGDGRIEWGKMWKLAKMAAKTYLEK"
     gene            complement(4541..5494)
                     /locus_tag="VF240_14565"
     CDS             complement(4541..5494)
                     /locus_tag="VF240_14565"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX7176485.1"
                     /translation="MRLFHGYENAEIARPTVLTLGVFDGLHLGHQLIARTVVEQARAL
                     EAVPTVITFDPHPRAVLHPESAPPLLQTLDQKVEALSLLGVEQTIVIRFTREFARVRA
                     AEFLRDVVRDRLQARAVYLGRGFAFGHEREGDIELLRRVSGELGFTASEAPEVRLRGR
                     RISSSLVRELLSAGKVNLARRMLGRPYGVEGRVVRGHERGRTLGFPTANLLPRNRVIP
                     RAGVYVTATLAGGVWRRSVTNVGVRPTFEAGAATSVETFVLGWEGDLYGDVVRVRFLR
                     RLRDEMKFASVEELKRQIDRDVGRAEEYFQHRNVRRSLAVV"
     gene            complement(5552..5704)
                     /locus_tag="VF240_14570"
     CDS             complement(5552..5704)
                     /locus_tag="VF240_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176486.1"
                     /translation="MKRLVAGGKDVRALEERQNLCPRLRRGDGVGGASRRFTSGYLLD
                     AAPRLK"
     gene            complement(5800..6570)
                     /locus_tag="VF240_14575"
     CDS             complement(5800..6570)
                     /locus_tag="VF240_14575"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7176487.1"
                     /translation="MKFTLLGTGTSTGVPSIGCDCETCVSDDPRDKRLRVSVLVEHAG
                     LQVLIDTSSDFRQQALRQGIRRLDAVLITHCHADHVFGLDDIRPLNFRHGPLGVYANE
                     RAWVDLRRIFKYVFEPSHFGGGLPQVVPHTVVDGAPFSLGPDLVVTPLEVIHGRLPVI
                     AYRFNDFAYATDLSEMPRATLDGLRDLDVLVLDCLRIHNPHPTHLTLERALSYIEELR
                     PRRTYLTHITHDVKHARDSARLPPGVEFGYDGLVISDE"
     gene            complement(6709..7305)
                     /locus_tag="VF240_14580"
     CDS             complement(6709..7305)
                     /locus_tag="VF240_14580"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX7176488.1"
                     /translation="MSEEKPTFKFTDRRLFNADGTPREDVREEREAELAAQAAATSAA
                     APPAPPPPTARAPEAPPQQTRPAAPSTGEPATAADSARGAVPGPGAMPSEEDDPQEFL
                     MVVEFVASFAAEALGMSTHPEAAGQAHVNLPLARQCIDMLATLERKTQGNLSAEEKRF
                     LDLILSQLRMHYVQLTQQRPAGGAAPRGFSGSDITGGR"
BASE COUNT         1386 a         2430 c         2563 g         1317 t
ORIGIN      
        1 cggtcgggct actgcctgtt tcgacctcac cccgcgggcg gtggcggcgc agccggttgg
       61 tagaaaatcc ccgcgcccgg cccgagcggc aggcccatca gataccagaa gatcaggagc
      121 agcgtccaca gtatgaagaa ggtcacggag tagggcagca tcgtcgccat cacggtgccg
      181 atgccggctc gcttgtcgta gcgttgcgcg aaggcgacga cgagcgggaa gtaggacatg
      241 agcggcgtga tgatgttggc caccgagtcg ccgacgcggt aggccgcctg ggtgagttcc
      301 ggcgagtagc cgacgagcat gaacatcggc acaaacaccg gcgccatcac cgcccacttc
      361 gccgacgcgc tccccagaat caaatccagc agcaccgtga tgcagacgaa cgcgatgatc
      421 atcgggatgg ggtagccgct gatccctgac gacttcaaaa actccgcgcc cttgacggcc
      481 gtaatcaccc cgaggtgcgt ccagttgaag aaggcgacga actgcgccgc gaagaaggcg
      541 agcacgaggt agagcgacat cgacgacatc gccttctcca tcgccttcat catgtcgccg
      601 tcggacttga tggtgcgcgc gccgatgccg taggccagcc cgatgagcgc gccggcgaag
      661 aagatgaagg cgacgacgcc cgtgaggaac ggggagtcga gcacgccgcc cgtcttgagg
      721 ttacgcaggt agccgctcgc ggggacaagg cccgcgagga cgaaggcgac gaagagcagc
      781 gccacgacga gcgcgaactt cagtccgcgt cgctcggccg cggtcaactc ctttagctcc
      841 tccggcttct cgtcgccctt gtactcgccc aggcgcggca cgatgacctt ctccgtgatg
      901 tacgtccccg agatggtaat catgatggcc gaggcggcca tgaagtagta gttcgccgcc
      961 ggggtgacga cgtagccggg gtggatgatg cgcgccgcct cctgcgtgaa gcccgcgagc
     1021 agcgggtcgg tggggcctaa caggaagttc gcgctgaagc cgcccgacac tccggcgaag
     1081 gcggcggcga gtcccgctat cgggtggcgc cccacggcga gaaagatcat cgcgccgagc
     1141 ggaatcagga ggacgtagcc cacgtctgag gccacgtgcg tcagcacgcc cgccaggacg
     1201 acgacgaagg tgagcagctt gcgcggcgcc gagagcacga gcgagcgcag caccgccgag
     1261 atgaggccgc tcccttcggc caccgcgatg ccgagcatcg ccacgagcac gacgccgagc
     1321 ggagcgaacc ccgtgaagtt cgtcaccaac cccgtcagga tgcggtgcag ccccgccacc
     1381 gtcagcaggc tctcggagcg gatgacctcc ttcgtgccgg ggtgcgtgac cgacacgccg
     1441 gcggcggccg caatcgccga gatgatgatg acggcggcgg cgaggatgac gaacagcgtc
     1501 gccgggtgcg gcaggacgtt gcccgcgcgc tcgatgaagc tgaggaaacg gttcatcgcc
     1561 gactcgcgcg cctgctgctg cggcggcggc tcggcgtcgt ctttcacgtg ctccatgttg
     1621 tgccttctcg cacggccgcg agggccgcgg cggggtggac tagctaatgg ccttcagatg
     1681 tagggccacg gcgggccggt tgccgccttt taaatcgcgg ggatggtaaa ccgggagcgg
     1741 ggccgggtaa agaactattt ttgaaaatcc ctacttcttt tccgcgacgt gtgcacgtag
     1801 ccaatcggta aactcctcgc gccccatttc gaccgccgcc aagcggagga ttactccctc
     1861 ctgatcgtca actcccgcgt caatttcatg tccgtttaag acgagaaaga cttccatcgc
     1921 ggcatggccc gcgcgcttgt tgccgtctac gaaaggatgg tttttgatta aagagaagcc
     1981 gagggccgaa gccttctcga cgacggtagg ataaaggtct tcgccgccga acgtcatcag
     2041 cggctgcgcc atggctgatt ccagaagccc gaggtcacgg atgcccggcg agccacccga
     2101 ctgttcgatg agccgccgat gaagctcgat caactctttc agcgtgagga atcgcatcac
     2161 gccagtcgcc tgtacaactc ctcgttcttt ccgagtacgt agtcggcggc gtcctcaaag
     2221 gcctcgtcgg gccgggccag caactcctcg atgctcaggc gggcgagttc ctcggggcgg
     2281 acgccgagcc gcgcggccag cttctgaagt tccagcagac gctccggcga aaggtcgatg
     2341 gtgatgctgc tcatccttca tcctccggcg acagattagt accgggcaat gttaccccag
     2401 ttctattttt ccaggtacgt cttggcggcc atcttcgcga gcttccacat cttgccccac
     2461 tcgatgcgcc cgtcgccgcc accacgcccg agcagacggt caacgaagag cttgcggaac
     2521 tgcttgccct cgcgccggag catgaactcc ttggcgtagg gcttggccgt gtcgaagaag
     2581 ctgaagccgg gatcggtgac gatgccgatg ccttccagag tcatcagcgc gcgcatgatg
     2641 taggtgaagt tgtagggcag gcggaacggg tagtcgtaca tcacttcggc cagatcgtag
     2701 gtcagctcct tgaagttcac gtcgccgagc ttgagattga tgtagtgctg gaaaagccct
     2761 tcaacaacgg ggcgcacgcg cgcggcgtcc acgcccggct tgaggaagtt gaggttgagg
     2821 aggtcttgcg cgagtcccga gatgtctcgc tcgacgacgt ggaagaaggc gtcgatcatc
     2881 aacgactgga gctgcggcgt gatgcggccc gtcatgccga agtcgaagaa ggcgacgcgg
     2941 ccgtcgtcca tcacgaggag gttgccgggg tgcgggtcgg cgtgaaagaa gccgtcctcg
     3001 agcaactgct tcaggtaagt gcgaaccagc agccggttga ccttgacggg cgagatgcgg
     3061 cgcgcgcgca agccctcgat gtccacaacc ttcgtgccgc ggatgaactc catcgtcagc
     3121 acgcgcggag aggtgtgcgt ccagtggatg aaggggacgt ggacgacgcg ccactcgcgg
     3181 aagttggcgc ggaagcgttc ggcgtttcgg ccctcttgcg cgtagtccat ctcctcgaag
     3241 atgacctgac gaaactcgcg gagcatcccc tcccagtcgg cgttctcgtt ggcttgcggg
     3301 tagcgcgtcg tgatgtggcg gacgagacgt tccagcacgg ccacgtcgaa agagatggtc
     3361 tcgcgcaagt tgggtcgctg caccttgacg gcgacctcct cgcccgtgtg cagacgcgct
     3421 cggtagacct ggcccagcga agctgcggcg accggctcgg cgtcgatctc ggcgtagagt
     3481 tccgagagct tgtggccgag ttcgctctcg atgcgcgcgt aggcttccga ggtggggaag
     3541 ggcggcacct gatcctgaag cgtggtgagt tctttgatgt aggggagagg caggaggtcg
     3601 gcgcgcgtgg cgagcgcctg cccgatcttg atgaaggtcg gccccagacc aatgagaccg
     3661 cgcctcagcc acgcggcctg accgtggagt ctggcctcgc gctcctcgga gccgcgcgcg
     3721 cctcggaaga cgaagtggcg caccgcgcgc gtgaggagat cggcggcctg tcggtcaacg
     3781 tcgcggctcc actcctgaaa gagggcgacg cggccacgcg cacgcgcctc ctcgcgcaga
     3841 cgcgcggcca cgcgccggtt gaacttgcgc cgcgcgtcgt agccgtcgag aaacaggaag
     3901 agcgcgagca cgccgagcac gcgcgctatc tggagcgtcc gccacacgcc gcgccacccg
     3961 cgcgcgcgca acgcaaactc gtcctcaggc gtcgccaccg cagcgtcccc gctcggcggc
     4021 gtcacacgaa cgcccccgcc cacgcgactc accgcgacgg ccttcgcccc tgcatcgtcg
     4081 gccgcgccgc cgttccccga cggcacgagc gagagagatt ctttcgcgtg accgttgccg
     4141 tttccattcc cgttcgcgcg cggcgcggcg accggcaaag gcgctcccgt ttgtttaccc
     4201 gctttttcgt ccatgatccc tgacttgcaa aagaagactg atgacggcct cagtttgaaa
     4261 aatgcgaatt aagacatgcg cgccgaatcc acatccggcc cgtttacggg cgaaactgct
     4321 gccccgcggc cccacccgcg cccctaaacg gtacggtctc gcggcccgcc gcgttcataa
     4381 aatcaaagcc cgtaccacag aaatagttca aagttcaaag tttaaagttc aaaggaatac
     4441 agggttcaaa cggaaatact ttgatgcgaa ggacgctgac ttggaacgtt gaactctgtg
     4501 tttcctttga actttaaact ttgaactttg aactctgtct ttagacgacc gcgagcgagc
     4561 gacgcacgtt acggtgttgg aaatactctt cggcgcggcc gacatcgcgg tcgatttgtc
     4621 gcttcagctc ttcgaccgag gcgaacttca tctcgtctcg caggcgccgc aggaagcgca
     4681 cgcgcaccac gtcgccgtag aggtcgccct cccatccgag gacgaaggtc tccaccgagg
     4741 tcgccgcgcc cgcctcaaag gtgggccgca cgccgacgtt cgtcacgctc cggcgccaca
     4801 cgcccccggc gagcgtggcc gtcacgtaga cgcccgcgcg cgggatgacg cggttgcgcg
     4861 gcagcaggtt ggccgtcggg aagccgagcg tgcgcccgcg ctcgtgcccg cgcacgacgc
     4921 gcccctccac gccgtagggc cgccccagca tccgccgcgc cagatttacc ttgcccgccg
     4981 aaagcagctc gcgcacgagg ctggagctga tgcgccgtcc gcgcagtcgc acctccggcg
     5041 cctccgaggc cgtgaagccg agttccccgc tcaccctccg caaaagctcg atgtcgccct
     5101 cgcgctcgtg cccgaaggcg aagccgcgcc cgagatagac ggcgcgcgcc tgaaggcggt
     5161 cgcgcaccac gtcgcgcaaa aactccgcgg cgcgcacccg cgcgaactcc cgcgtgaagc
     5221 ggatgacgat ggtctgttcg acgccgagta agctgagcgc ctcgaccttc tgatccaacg
     5281 tctgcaacag cggcggcgcg ctctccgggt gcagcacggc gcgcgggtgc gggtcgaagg
     5341 tgatgacggt cggcaccgct tccaacgcgc gcgcttgctc gaccacggtg cgcgcgatca
     5401 actggtgccc cagatgcagc ccgtcgaaca cgcccagcgt cagcaccgtc ggccgcgcaa
     5461 tctccgcgtt ctcgtatccg tggaagagtc tcataagcag gtgatgagtg atgagtgatg
     5521 agtgataagt gataagagcc tgtttgaaaa ctcatttcag ccgcggagcg gcgtcgagaa
     5581 ggtagccaga cgtgaaacgt ctggatgcgc cgccgacacc atccccgcgt cggagacgcg
     5641 ggcagaggtt ctggcgctcc tccagagcgc ggacatcttt cccgcccgct accagacgtt
     5701 tcacgtctgg ctaccttctc ggtgcgcctc cggcgcataa atttgagttt tcaaacaggc
     5761 tctaagtgat aaaaaaaccc gcttctctct tatcacttat cactcatcac ttatcactaa
     5821 tccgtcgtag ccgaactcga cgccgggggg caggcgggcg gagtcgcgcg cgtgcttcac
     5881 gtcgtgggtg atgtgcgtca agtaggtgcg gcgcgggcgc aactcttcga tgtacgaaag
     5941 cgcgcgctcc agcgtcaggt gcgtcgggtg cgggttgtgg atacgcaggc agtcgagcac
     6001 gagcacgtct aagtcgcgca ggccgtcgag cgtggcgcgg ggcatctcgc tgaggtcggt
     6061 cgcgtaggcg aagtcattga agcggtaagc gatgacgggc aggcggccgt ggatgacttc
     6121 gagcggcgtg acgacgaggt cggggccgag cgagaacggc gcgccgtcaa caactgtgtg
     6181 cgggacgacc tgcgggagac cgccgccgaa gtgcgatggc tcgaagacgt acttgaagat
     6241 gcgccggaga tcgacccacg cgcgctcgtt ggcgtagacg ccgagggggc cgtggcggaa
     6301 gtttaaggga cggatgtcgt cgaggccgaa gacgtggtcg gcgtgacagt gtgtgatgag
     6361 cacggcgtcg agccggcgga tgccctgacg cagggcctgc tggcggaagt ccgaggaggt
     6421 gtcgatgagc acctgaagcc ctgcgtgctc gacgaggacg gagacgcgca ggcgcttgtc
     6481 gcgcgggtcg tcggagacgc acgtctcgca gtcgcagccg atggacggca cgcccgtcga
     6541 tgtccccgtg cctagaagcg tgaatttcat caaggccgta aatcgtaaat cgtgaatcgt
     6601 gaatcgtaaa tcgtggttga gggtgtttca acctgagcgg cgccgttcaa gccgaacgag
     6661 cgccatcccc atcgattgac gattcacgat tcacgattga cggctttttc atctcccgcc
     6721 cgtgatgtcg ctgcccgaga agccgcgcgg ggccgcgccg ccggcggggc gctgctgcgt
     6781 gagttggacg tagtgcatac gcagttgcga gaggatgagg tcgaggaaac gtttctcctc
     6841 ggcgctgagg ttgccctgcg tcttgcgttc gagtgtggcg agcatgtcga tgcactggcg
     6901 ggcgagcggc aggttgacgt gcgcctgacc ggccgcctcc gggtgcgtgg acatccccag
     6961 tgcctcggcg gcgaaagagg cgacgaactc gacgaccatc aggaactcct gcgggtcgtc
     7021 ttcctcactc ggcatcgctc cggggccggg gacggcgccg cgcgcggagt cggcggccgt
     7081 ggccggctcg ccggtggacg gggcggcggg tcttgtctgc tgcggcggcg cttcgggcgc
     7141 gcgggccgtg ggggggggcg gcgcgggcgg ggccgccgcg gacgtggccg ccgcctgtgc
     7201 ggccaactcg gcctcgcgtt cctcgcgcac gtcttcgcgc ggcgtgccgt cggcgttgaa
     7261 cagccgccgg tcggtgaatt tgaaggtggg cttttcttcg ctcatatgga cgttcgggtg
     7321 ccctcctgag aaggctcaag ctcgtgaatt ggactactgt tttccggctc agaggatggt
     7381 agcactcact ccgcgcggtc gcaagaaacg gtgattcgcg tatcgtcgaa ctcgcctgcc
     7441 gatagccccg tcaggggcga aagagtgtag cccacggcgt gagccgtggg gacaggcgcc
     7501 agaaaacatt agaagccctg gaggggcgaa agaatcgcag acttgcgcgt tgcgccggtc
     7561 gcgattcttt cgcccctccg gggcttggtt cgtattacgc tgccgattcc cacggctcac
     7621 gccgtgggct acattcttcc gcccctgacg gggctaacgg ctcgcgagtt cgacgacacg
     7681 cgaatcaccg tttctt
//
LOCUS       DASVES010000358         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_637564, whole genome shotgun sequence.
ACCESSION   DASVES010000358 DASVES010000000
VERSION     DASVES010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637564"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VF240_14585"
     CDS             complement(<1..441)
                     /locus_tag="VF240_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176489.1"
                     /translation="MSRIGKSVPRKEGRDKVTGRAAYVDDIRLPGMLHGATVRSPAAR
                     GRVKEIVFDPSIPWDEFTIVTAKDIPGANCVALILLDQPYLADGVVNHPEEPIVLLAH
                     EDKYLLEEARRSVRVEVEPLPAVFTIEESLQKKEIIWGEDNIFKS"
     gene            complement(608..>2774)
                     /locus_tag="VF240_14590"
     CDS             complement(608..>2774)
                     /locus_tag="VF240_14590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7176490.1"
                     /translation="MLALVLMVGVVIDDAIVVLENIFRFIEEKKMHPAEAAREATKDI
                     GLAVLATTLSLVVIFLPVSFMSSISGRFLYQFGITAAVAIMVSLLVSFTLTPMMSSRM
                     LRAEDAGKGHSGGHDDEGGEVAGSRAGFYARIDRFYTRLLAFSMRHRLAVSVLAVAVV
                     ASSVPLYRAVKQEFIPTNVDEAEFEVSLNAPEGANLAVMTEAMQVLEKELMTTPGVTM
                     VLANAGGGFLGGINQAGAYVRIAPHEERTLSFTKVWNKTLEGKPWEAFRGNYTQQDVM
                     QEARRRLRKFSPMRGSVRNAPSFNFGSGGRSDIDFVLRGPDILELDRYANELLARAEK
                     LGGIVDADTSLKLNKPELRVEIDRQRAADLGVDTSDIATSLRIMVGGDQEASRFQDES
                     VNESYDVQLRLTESDRSEAAGIERLYVPSTRGGLVRLDNLVRIKRDYSPSRIDRLDRE
                     RQVSVRATVAPGFALADRIEAMRGAVREMNLPAAYTTTISGRGRELERTFNEFIWAFL
                     LSVIFMYMILASQFESVIHPITILLSLPLAVPFALLSQYLTGNTLNLYSALGILVLFG
                     VVKKNSILQIDHMNNLRAKGLERLEAIMQGNRDRLRPILMTTLALVAGMLPLALGTGP
                     GAEERRSIAIVVIGGQTLSLLLTLLVTPVAYSLFDDLAALGKWRRASSRITAFPARLA
                     TGLRAVFARTRVKKDDASDDDADVIPEAHGKHAASGGGD"
BASE COUNT          491 a          837 c          969 g          477 t
ORIGIN      
        1 cgacttgaag atattgtcct ctccccagat gatctctttc ttctgaagcg actcctcgat
       61 ggtgaagacg gcgggcagcg gctcgacctc gacacgcacg gagcggcgcg cctcttcgag
      121 cagatacttg tcttcgtgcg cgagcaggac gattggctct tcggggtggt tgacgacgcc
      181 gtcggcgaga tatggctggt cgaggaggat gagcgcgacg cagttcgcgc cgggaatgtc
      241 tttcgccgtg acgatggtga attcgtccca ggggattgaa gggtcgaaga cgatctcttt
      301 gacgcgcccg cgcgcggcgg ggctgcgaac ggtcgcgccg tggagcatgc cgggcaggcg
      361 gatgtcgtca acgtaggcgg cgcggccagt caccttgtcg cgcccctcct tgcgggggac
      421 ggattttccg attctgctca tggacatcag ggaggctccg ggtcgggcta ctttacaata
      481 actctccggc agaaggaaca cggaataaac gaatgcgacg gattgacaca gattcgatcc
      541 gtgctcatcc gtcgcatccg tctaatccgt gttcccctca cctgaccggc gtcagtcgaa
      601 gctcgaatca atcgccgccg ccggacgcgg cgtgtttgcc gtgcgcctcg ggaattacgt
      661 cggcatcgtc gtcgctcgcg tcatccttct tgactctggt tcgggcgaag acggcgcgca
      721 acccggtcgc gagtcgtgca gggaaggctg taatgcgcga cgacgcgcgc cgccacttgc
      781 cgagggcggc gaggtcatcg aagagcgagt aggcgacggg cgtgacgagg agcgtcagga
      841 ggagcgagag cgtctggccg ccgatgacga cgatggcgat ggagcggcgc tcttcagccc
      901 ccggccccgt gccgagggcg agcgggagca tgccggcgac gagcgcgagc gtcgtcatca
      961 ggatggggcg caggcggtcg cggttgccct gcatgatcgc ctccagccgc tccagaccct
     1021 tcgcgcgcaa gttgttcatg tggtcgatct ggagaatcga gttcttcttg acgacgccga
     1081 acaggacgag gatgccgagc gccgagtaga ggttcagggt gttgccggtc agatactgcg
     1141 agagcagcgc gaacggcacg gcgagcggca ggctcagcaa gatcgtgatg gggtggatca
     1201 ccgactcgaa ctgcgaggcg aggatcatgt acatgaagat gaccgagagc aggaacgccc
     1261 agatgaactc gttgaaggtg cgctcaagct cgcgcccgcg cccggaaatc gtggtcgtgt
     1321 aggcggccgg gaggttcatc tcgcgcacgg ccccgcgcat ggcctcgatg cggtcggcga
     1381 gcgcaaagcc gggcgcgacg gtggcgcgca cggagacttg ccgctcgcgg tcgaggcggt
     1441 cgatgcgcga agggctgtag tcgcgtttga tgcggacgag gttgtcgagg cggacgaggc
     1501 cgccgcgggt cgagggcacg tagaggcgct cgatgcccgc cgcttcgctg cggtcgctct
     1561 cggtgaggcg caactgcacg tcgtaggact cgttgaccga ctcgtcctga aagcgcgaag
     1621 cctcctgatc gccgcccacc atgatgcgta aagaggtggc gatgtcggag gtgtccacgc
     1681 cgaggtcggc ggcgcgctgg cggtcgatct ccacacgcag ctccggcttg ttgagcttga
     1741 ggctggtgtc ggcgtccacg atgccgccga gtttttcagc gcgggcgagc agctcgttgg
     1801 cgtagcggtc gagttcgagg atgtcggggc cgcgcaggac gaagtcgatg tcggagcggc
     1861 cgccgctgcc gaagttgaac gacggcgcgt tgcgcacgct gccgcgcatc ggcgagaact
     1921 tacgaaggcg ccggcgcgct tcctgcatca cgtcctgttg cgtgtagttg ccccggaatg
     1981 cttcccacgg tttgccttcg agcgtcttgt tccagacctt ggtaaacgag agcgtgcgct
     2041 cctcgtgcgg ggcgatgcgg acgtaagcgc ctgcctgatt gatgccgccg aggaagccgc
     2101 cgcccgcatt ggcgagcacc atcgtgacgc ccggcgtcgt catgagttcc ttctccaaca
     2161 cctgcatcgc ctcggtcatc accgcgaggt tggccccctc cggcgcgttc agagaaactt
     2221 cgaactcggc ctcgtccacg ttcgtcggga taaactcctg cttcaccgcg cggtagagcg
     2281 gcacgctcga agcgacgacc gccacggcga ggacggagac ggcgaggcga tggcgcatcg
     2341 agaaggcgag caggcgcgtg tagaagcggt cgatacgcgc gtagaagccg gcgcgcgagc
     2401 cggccacttc gccgccttca tcgtcgtgcc cgcccgagtg gcccttcccg gcgtcctcgg
     2461 cgcggagcat gcgcgaagac atcatcggcg tcagcgtgaa ggagacgagc aagctaacca
     2521 tgatggccac ggccgccgtg atgccgaact gataaaggaa gcgccccgag atcgacgaca
     2581 tgaaggagac cggcaggaag atgacgacca gagacagcgt cgtcgccagc acggcgagac
     2641 cgatatcttt ggtcgcctcg cgcgccgcct ccgcggggtg catcttcttc tcctcgatga
     2701 agcggaagat attttcgagc acaacgatgg cgtcgtcgat gaccacgccg accatgagga
     2761 cgagcgcgag catc
//
LOCUS       DASVES010000359        20185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_831614, whole genome shotgun sequence.
ACCESSION   DASVES010000359 DASVES010000000
VERSION     DASVES010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831614"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..911)
                     /locus_tag="VF240_14595"
     CDS             complement(129..911)
                     /locus_tag="VF240_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX7176491.1"
                     /translation="MKIQIARDARPQVRALLLAAGLSVALWFIPYAWVLTYPFQLFVT
                     FIHEGGHALAAVLTGNSVRSLSVSLDTSGLTETWVAPGGFFSGFFISSAGYLGAMAFG
                     AMLLWLVRRRVKSSVVLYGSAAVITALTVVFGFLVPLTNFSFSPFTILSGGLITVGLV
                     AAARFLSRPAASFLVSFLAVQCVLNAIFNLRDLAVLSLGTGAHTDAGNMQAATGIPAI
                     FWALMWAAVAFVILSLALRAYSVAREKPSQPDLPFEDSPLEV"
     gene            complement(1036..2760)
                     /locus_tag="VF240_14600"
     CDS             complement(1036..2760)
                     /locus_tag="VF240_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176492.1"
                     /translation="MKRTLPLVASLAAATLLAFLCRTPPARAQEAHEHQHEPSEKLGR
                     VNFNVSCNPQARKQFNRAVAWLHSFEYEEAEKVFNEVTVTDPRCGMGYWGIAMSNYHP
                     IWAPPSAAELKKGLSAIEKAKAAGSLTGRERDYIAAIEVFYKDSDKLDHRTRAFAYSE
                     AMERLYRRHPSDREAGVFHALSLVATGMMANDKGYANEKKAARILNRVLAREPQHPGV
                     THYLIHGYDYPALAHLALPAARSYAKIAPASAHALHMPSHIFTRLGLWREAIRSNIDA
                     KAAAKAHAVRNRLPGVWDEQFHAMDYLAYAYLQGAQDKQAEGVLDELKKIQRAEPENF
                     KVAYASTAIPARFALERRQWNEAARLPLPAGTLGTFPWQRFRWAEAHIHFARAVGAAR
                     TGDTASARQEVEKLAAIRQALVKVKGDYDWATQVEIKHQVASGWLAHAEGKHEEALRL
                     MRAAAELDDATEKHPVTPGTILPAREQLGELLLELKRPTALQEFETSLRSAPNRFNGL
                     YGAARAARLAADQKRAKIYYAKLMALCREADSVRPEIEEAKAFLAGANVAATRLESRR
                     PGRHHAHR"
     gene            complement(2822..3277)
                     /locus_tag="VF240_14605"
     CDS             complement(2822..3277)
                     /locus_tag="VF240_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7176493.1"
                     /translation="MQIEIDDLRGSEIHELLQEHLRSMALHSPPESVHALDIEALRRP
                     EVTFWTVWENGELLGCGALTELDSRHAEIKSMRTSSSHLRKGVAKNLLSHILEEARRR
                     GYSRVSLETGSMEAFAPARQLYANFGFTYCEPFADYVEDPYSVFMTREL"
     gene            complement(3384..4061)
                     /locus_tag="VF240_14610"
     CDS             complement(3384..4061)
                     /locus_tag="VF240_14610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7176494.1"
                     /translation="MAETMSTIRTDFDRLALLSGEEWDHSSQYHDFLLRRVPSPCRES
                     LDIGCGAGVFSRLLAKSSERVLALDLSPNMIRIARERSASFPNIDFQVADVMTRELPA
                     ERFDCIATVATLHHLPMTEVLPKIKSALKVNGVLLILDLFQNEGLVDALTSALAAPVS
                     MGLKLIRHGRVMAPRHVREAWAEHGRHDSYLTMAQVHQVCGDMLPGAEVRKHLLWRYS
                     IIWKKVA"
     gene            complement(4122..5174)
                     /locus_tag="VF240_14615"
     CDS             complement(4122..5174)
                     /locus_tag="VF240_14615"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX7176495.1"
                     /translation="MVNRQSIAHTPTCDSPFKLILPQARFTIYYLPFTALKVMNIASN
                     ILDSIGDTPIVPLRKIVPPGSARVLAKLEWANPTGSMKDRMAKAVIEAAEASGRLQPG
                     GAVVEYTGGSTGISLAMVCAAKGYRLQIVTSDAFSEEKIRTMQAFGAQVTCLPSDNKK
                     INEKLIKDMIDTAHRISQQPGHWWVNQISNQDAAAGYYSMGEEIWEQTDGEVDAFVHS
                     VGSAHSLHGAAKALRQHKPELHVAAVEPSETAVLSGKSPGSHKIEGIGIGFIPPHWKP
                     DEVDEILTDSTEEAIQMCRRLAREEGIFAGTSSGLNVLAALRVAERLGPEATVVTLMV
                     DSGLRYMSTDVYRADQ"
     gene            complement(5182..5319)
                     /locus_tag="VF240_14620"
     CDS             complement(5182..5319)
                     /locus_tag="VF240_14620"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX7176496.1"
                     /translation="MSWSSRYFGGLPVEAAAEVLKVSSDTVTREWRRAKAWLHREMQR
                     T"
     gene            complement(5481..5954)
                     /gene="moaC"
                     /locus_tag="VF240_14625"
     CDS             complement(5481..5954)
                     /gene="moaC"
                     /locus_tag="VF240_14625"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX7176497.1"
                     /translation="MSELSHVDEEGRLRMVDTGAKALSARRARASARVLMSPETVAAL
                     RERRTPKGDPLETARLAGIMAAKRTADLIPLCHTLPLTHADVRAEIRDDGVHIEAEAS
                     TVAQTGVEMEALTAAAVAALTVYDMCKALDKGIQITDVRLEEKTGGKSGDWKRQG"
     gene            6148..6744
                     /locus_tag="VF240_14630"
     CDS             6148..6744
                     /locus_tag="VF240_14630"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX7176498.1"
                     /translation="MKKLLFAMVMTCVGLGISAAEVSAQQNPMVGGAAMYKSKNIVEN
                     ALNSPDHTTLVAAVKAAGLVETLAGKGPFTVFAPVNSAFDKLPAGTVETLLKPENKDM
                     LTQILTYHVVPGKMDAKKIMSAIKKGGGTASFKTVSGNTLTASMSGNMVVLRDEKGGM
                     STVTIANVMQSNGVIHVVDTVLMPGEKMMMSDGMKKTM"
     gene            complement(6951..8168)
                     /gene="glp"
                     /locus_tag="VF240_14635"
     CDS             complement(6951..8168)
                     /gene="glp"
                     /locus_tag="VF240_14635"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX7176499.1"
                     /translation="MIPVAEAVRIVLEKTEALDAERVALREAPGRVLAEPVVADTDLP
                     PFDRAQMDGYAVRSTDLAQLPARLRVVGEAAAGQGWRGTLRAGEAVRIMTGAPLPTGA
                     DGVQQVELARESEGGDEVEIMRATVPGQFFVPRASEIKKGAVVLRAGEEVGAASVAAL
                     ASFGYAEVSVRRRPRVAVLATGAELVRVDERPGADQIRDSNTHSLAAYAALAGAVVEQ
                     MPFAGDEPELLQRLIADASTRSDVVVLSGGVSMGRYDFTKASMRALGAEVFFERVALR
                     PGKPSVFARLPGAKQTLLFGLPGNPVSVSVTFNLFARTALRAMQGALEPMLKEERGVL
                     SRRTRGTVERTSYLPARLFTDEHGRLMAEPLKWGGSSDFVAFARAEALVIVAADVREV
                     EAGSVVQLVRLPV"
     gene            complement(8235..8996)
                     /locus_tag="VF240_14640"
     CDS             complement(8235..8996)
                     /locus_tag="VF240_14640"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7176500.1"
                     /translation="MHTERIKPERAARAIFAERYDGARVIFLAGSVVRGEATPASDLD
                     LVVVYERLPHAYREAFFHAGWPVETFVHDPETLRYHFESDRRRGIPSLMRMVTEGIEI
                     PGASEFSAGVKRSAAELLEAGPPRWDAAELMRRRYRLSDWVDDIRYPRTPEELVASGT
                     FLYADLADFFLLSQNLGTAHSKTIPRRLREVDAGFAERFGNAFEALFVEKQPAPVITL
                     VEEVLEQFGGILFEGFRRNSAPAERALSEAPPADA"
     gene            9532..10932
                     /locus_tag="VF240_14645"
     CDS             9532..10932
                     /locus_tag="VF240_14645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176501.1"
                     /translation="MTRRSDLRLIVTFALLTFLLTAGVILAWENLLLPPFYSWVDARY
                     PGEANVQTRWNIQQRVEHFFISITVDVVVVTLLVGMLRAQQRRLAQSEGRYRSLFEHA
                     RDGIGVVHAGSRRLIEANSKFCEVLGCKPPEVIGRKLDEFLRTPDAPRPPLISELLDS
                     GKSGEGEMSILTAGGASLPVSFSFHSLSTEGEEELLTLIIRDLSSRKRLEAENEEIQR
                     QLFQSSKLASLGELSAGVAHEINNPLNGIINFAQLLKDEPAPRSEFEQQMIDGVIDEG
                     ERIAQIVRGLLTFARNDSQELHRVRLEDAVKASVALFGRQLEKDGIRVEVEFAADLPP
                     MRADASRLRQVVVNLISNAHHALREKPPRAGEQKVFRISARRVVAAAGVVVRTEFYDN
                     GAGIRREDLGKVFDPFFTTRRETGGTGLGLSITFGIIRDHGGTITVASEEGKFTRFTM
                     DLPAAGAYEIEGVEYV"
     gene            10925..12085
                     /locus_tag="VF240_14650"
     CDS             10925..12085
                     /locus_tag="VF240_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176502.1"
                     /translation="MFRVLLVDDEPSLRFTMSEFLKRDGYEVRAVSDYAEAAAVGNEH
                     VDVAVVDVNLPGGSGVDLLRQFSGREPYVPVIMITGEPTLSLLPDIVRAGAYDFIAKP
                     VVKDVLLRAVARASEKKRLTEEKGRLEQEVRRHAEELETRVAERTAELLAAHTRLDRQ
                     EKIASLGRIAAQVAHEVKNPLAGLLLYSMHLRDRVAAKLPDDELRLIDKIIETTRYMD
                     RTCEQVLDFARPISLTPLPADLNLIVADVLHILEPQLAAGAIERRLELGEPEVAGVLD
                     QTSIRGALTNLILNAVQAMPSGGTLAVKTLRADGALKVEVSDTGVGMNEEQLGNIFEP
                     FYSTKSRGLGLGMPYVRKVVEEHRGTIQVESRPSAGTTIRVSLPVGAEGVSA"
     gene            12082..13479
                     /locus_tag="VF240_14655"
     CDS             12082..13479
                     /locus_tag="VF240_14655"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7176503.1"
                     /translation="MSRQRILVVDDEASVAGALELILGEQGYEVSTAPTVREAEALLA
                     GRPFDLAFVDLRLPDDDGIGLLQRIRGDAPDTQVILMSAHGSLDITIEAIKRGAFYYV
                     EKPFTPEQVVMLAKRALQFEEIRRENRLLKSALGGDGDDFGLVGNHAKIRQVRAAIRT
                     AAPSDASVLIEGESGTGKELIAAALHSQSARSRGPFIRINCAAIPSELIESELFGYRR
                     GAFTGADRDKRGLVEAAAGGTLLMDEIGEMPVYLQTKLLRVLQERRVRRLGDEQEFDV
                     DFRLVSATNRDTAQMVAEGTLRKDLYFRISTVRIKMPPLRERLDDLVVLAERFLLRYA
                     EKYQKRLRGISTAALSLLTRYDWPGNVRELESAVEHAVLFAQEEQIMPEDLPEQLHDV
                     RAASFRCVIPPYVTMEEIEREAIAQTLERTGGNVKKTAQILSYHRPTLYRKLKKFGLL
                     RDDLTHAQADVARGE"
     gene            13653..14036
                     /locus_tag="VF240_14660"
     CDS             13653..14036
                     /locus_tag="VF240_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7176504.1"
                     /translation="MGKTEILIIDDERSVADALKVILESEGFDVTLAASGRDGIERAR
                     QEKFRLAITDLRLPDMSGLEVLQTLRAENPGLRVILTTSYGTPKILAEAIGFGAEGTL
                     DKPFAPSEIVRLITSVLERTPDARS"
     gene            14490..16499
                     /locus_tag="VF240_14665"
     CDS             14490..16499
                     /locus_tag="VF240_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX7176505.1"
                     /translation="MKKRSHILLTAALLLCSPALTSTTTRAQEDKTAPDAKASVQKDA
                     KVAPVATPAPVRGPQKIVKDGVEVEFTIEPVTPSGASPELLAGQDAVVRFRVRDTATG
                     TPLSGVRPSAWINRRTGSAPPDAQQCRELIQSYLQGSLRARPDVDLNAYYILALNEEP
                     NLSVIDPLLGFGGSKLVTLVFLKSPGADWALTADRSRLFVSMPAAGLVAVVDTTTWKV
                     VADVSAGARPTRVRLQPDGKYLWVANDAEGAGASGGVTVIDTASLKVAASIPTGAGTH
                     DLAFSEDNRAAFVTNRDEGTLSIIDVRKLEKVKDIKTGAPASALAFSPLSKALYVANE
                     TDGSLSVVDGQSYELRARIAAQPGLRSVRFAPGGRWGFVPNATRSVVHIFDASTNRLV
                     HDVPVGKTPDQVAFTDAFAYVRSLGAEEVSAIRLSTVGKEPDVVKFPGGQAAPGTSRS
                     EALSADAFVPAPESSTMLLANPADRMIYYYSEGMAAPMGNFQNYKREPRAIKVVDRSL
                     REESLGNYATTLRLPADGIYNVSLLMDSPRVVHCFEATAKPNPAMKQEQKTALRVEYL
                     NKEQPALRPGASYKIRFRLTDAATGQPKEDLKDVRVLFFLSPGIWQTRDFARSVGKGI
                     YELEVTPPQEGVYMIFVESRSQGVSFRHLPHLTLSADAAPPGSSQ"
     gene            16604..17407
                     /locus_tag="VF240_14670"
     CDS             16604..17407
                     /locus_tag="VF240_14670"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7176506.1"
                     /translation="MNSKTLSSFARRASLVLAAALVAGASDAPTVRAQKKKKPQPARV
                     ESPEASAHYSCPMHPSIKSAKPGRCPKCKMELRLTKDEPAEAKTVIAKTPTPAASDSV
                     SGMNMPDTELLDQDGRKVRFYTDLVKDKTVVINFIFTTCTTVCPPLGATFARVQRELG
                     ERTGRDVYFISVSVDPVTDTPERLKAWGAKFKAGPGWTFVTGAKPRVNEVLAALSASS
                     ASREDHTPTILIGNDAKRQWTRAYGLTAPSKIVQIINDAASGRLASNTK"
     gene            17452..18108
                     /locus_tag="VF240_14675"
     CDS             17452..18108
                     /locus_tag="VF240_14675"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7176507.1"
                     /translation="MIKRVLLVAAVCLSLASTAAAQQTAKPPAPPPDPHAGHGAPARA
                     EQEPTVAAKYFTDVELVNQDGQKVRFYSDVLKGKTVVVNAFFTTCTSVCPPMNRSMEK
                     IQEAFGDRVGKDVFLVSLTVDPATDTPVRLKEYAAKFHAGQGWMFLTGKKENLDWALY
                     KLGQYVENKDDHKTVIIIGNEATGLWKKAFGMAKVEELIKLVKEVVDDKGDGKTSAAQ
                     "
     gene            18143..19777
                     /locus_tag="VF240_14680"
     CDS             18143..19777
                     /locus_tag="VF240_14680"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7176508.1"
                     /translation="MLQSLKLTITLLALAFFAAFTLLGAPAGAESWAVQGTTTTTARP
                     QPLTEQERRGRSLYLRGESASGSEIVAVVGELDVPASTMTCAGCHGARGEGKTEGGVT
                     AGSLVWSHLVKPYGHMHPSGRKHANPFTESSFIRAVSNGVDPEGNDLLAAMPRYRMSA
                     ADMADLIAYLKRIETDLDPGLSDTSLRVGVVLPAKGVLAETGAAMKDVLAAYFDGVNS
                     RGGVYNRKIELRVAEAGDDGAATAAAARAFARQEQVFAFVGGISVGADAELAALARDE
                     EIPFIAPSTLTPQTGTPPNRYVFYLLSGVAEQARALVNFAASQKELNKKRAAIVYTEG
                     TLAVAAAAATEDQAKKSGWAGVTKQGYKADGFDAAQIVRRLKQEGAESVFVFGGEASA
                     LVKEAAALGWTPHVFFLGMMAGRDFPGEAPAGFKDRVFLSFPTIPADVTPAGVAEFRA
                     LQEKYKFAPRHVAAQLSAFAAAKIFVEGLQRVGRDLSRERLVTALEGFYDFETGVTPR
                     LTFGPNRRVGAAGAYVVTIDPEKKEFVPASGWVKAY"
BASE COUNT         3746 a         6602 c         6409 g         3428 t
ORIGIN      
        1 tcggcgcgtg cgccgtcgtc gtctgagaag ggaggaggcc gtcggatggg gcgtgcggcg
       61 tcggcggcgg ggccgtcgtc gccgcctctt ccggcggcct ctcgaatgct gcgctcacag
      121 tttagttttc agacttcgag cgggctgtcc tcgaagggca ggtcgggctg gctcggcttc
      181 tcgcgcgcga ccgagtaggc ccgcagagcc agcgagagga tgacgaaggc gacggccgcc
      241 cacatcagcg cccagaagat ggcggggatg cctgtggccg cctgcatgtt gccggcgtcg
      301 gtgtgcgcgc ccgtgccgag cgagagcacg gcgaggtcgc gcaggttgaa gatggcgttt
      361 aagacacact gcacggcgag gaagctgacg aggaagctgg ccgcggggcg actgagaaac
      421 cgggccgccg cgacgaggcc gaccgtgatg agtccgcccg agagaatcgt gaacgggctg
      481 aaggagaagt tcgtgagcgg cacgaggaag ccgaagacga ccgtgagggc cgtgatgacc
      541 gccgccgagc cgtagagcac gacggacgat ttcacccggc gacgcacgag ccacagcaac
      601 atcgcgccga aggccatcgc cccgaggtag cccgcgctcg aaatgaaaaa gcctgagaag
      661 aagccgcccg gcgcgaccca cgtctcggtc agcccgctcg tgtcgagcga gacgctgagg
      721 ctgcgcacgg agttgcccgt gagcacggcg gcgagcgcgt ggccgccctc gtggatgaag
      781 gtgacgaaga gctggaacgg gtaggtcaga acccacgcgt aggggatgaa ccagagggcg
      841 acgctcaggc cagcggccag aagcagcgcg cgcacctgcg ggcgcgcgtc ccgcgcgatt
      901 tgaatcttca tctttggaaa atctccactc gaaagaactc aaacatttct cgctgtggtg
      961 tacgacattc tacgacgaag cgttccgtgg cgcaataagc agttgctttt caacctgtta
     1021 cgtcattgac gactcttacc tgtgtgcgtg atgcctgccc ggccgtcggg attcgagcct
     1081 cgtagccgca acattcgcgc cggcgaggaa cgctttcgcc tcctcgatct cgggcctgac
     1141 gctatcagcc tcgcggcaca gcgccatgag cttagcgtag taaatcttcg cccgcttctg
     1201 atctgccgcg agccgggccg cgcgcgccgc gccgtaaagc ccattgaagc gattgggcgc
     1261 gctgcgaagc gaggtctcga actcctgcaa ggccgtaggc cgcttcaact ccagtagtag
     1321 ctcgcctagc tgctcgcgcg ccggaagaat cgtccccggc gtcacggggt gtttttcagt
     1381 cgcgtcgtcc aactcggccg ccgcgcgcat cagccggagc gcttcttcat gtttgccctc
     1441 ggcgtgcgcc agccagcccg aagcaacctg atgcttgatc tcgacctgtg tggcccaatc
     1501 gtaatcacct ttaactttaa cgagcgcctg ccggatggcc gccaacttct cgacctcctg
     1561 tcgagccgaa gccgtatcgc cggtgcgcgc cgccccgacc gcgcgcgcaa agtggatgtg
     1621 ggcttcggcc cagcggaagc gctgccaggg aaacgttccc aaagtcccgg cgggcaatgg
     1681 caacctggcc gcttcgttcc attgccgccg ttcgagcgcg aagcgtgcgg ggatggccgt
     1741 ggaggcatag gccaccttga aattctccgg ctcggctctc tgaatcttct tcaactcatc
     1801 gagcacgccc tccgcctgtt tgtcctgcgc gccttgcagg taggcgtaag cgagatagtc
     1861 catcgcatga aactgctcat cccacacgcc gggcagacgg tttctgacgg cgtgagcctt
     1921 cgccgccgct ttggcgtcga tgtttgagcg tatcgcttct cgccacaagc ccagccgcgt
     1981 gaaaatatgt gaaggcatgt gcagggcgtg ggctgatgcg ggcgcgatct tcgcatagct
     2041 ccgcgcggcg ggcagcgcca gatgagccag cgccgggtag tcgtagccat gtatgaggta
     2101 gtgagtgacc cccggatgct gcggctcacg cgccagcacg cgattcaaaa tccgcgcggc
     2161 cttcttttcg ttggcatagc ccttatcgtt cgccatcatc cccgtcgcga cgagcgacag
     2221 cgcatggaag acgccggcct cacggtctga cgggtggcgc cggtaaagcc gctccatcgc
     2281 ttcactgtaa gcgaacgcgc gcgtccgatg atcgagcttg tcggagtctt tgtaaaaaac
     2341 ctctatcgcc gcgatgtaat ctcgctcgcg tccggttagt gagccggcgg cctttgcctt
     2401 ttcgatggcg ctcaatccct tcttcaactc cgccgcgctc ggcggcgccc agatgggatg
     2461 gtagttgctc atcgcgatgc cccagtaacc catgccgcat cgcgggtcgg tgacggtgac
     2521 ttcgttgaac accttctccg cctcttcata ctcgaaagaa tggagccagg cgacggcgcg
     2581 attgaattgc ttcctggcct gtgggttgca ggagacgttg aagttgaccc gcccgagctt
     2641 ttctgaaggt tcgtgttgat gctcgtgcgc ctcttgcgct ctggcaggcg gggtgcgaca
     2701 gagaaaggcg aggagagtgg ccgcggctaa tgatgcgacg agcggaagag tgcgtttcac
     2761 aggttcttct cctttggggg aagcggaagg gcgaggggcg gtagagcgtc taaaccaaaa
     2821 ctcaaagctc tcttgtcata aatacgctat acgggtcttc aacataatcc gcaaacggct
     2881 cgcagtatgt aaagccaaaa ttagcgtaga gttgacgggc aggcgcgaaa gcttccatcg
     2941 atcccgtctc taaacttacc cggctgtaac cacggcgtct ggcttcttca aggatgtgac
     3001 tgagaagatt tttcgccacg cccttcctaa gatgtgacga agaagtccgc atggatttga
     3061 tttcggcatg tcgagagtcc agctccgtga gtgctccaca gccgagcagt tcgccgtttt
     3121 cccacacggt ccaaaacgta acttccggcc ggcgtaatgc ctcaatatcg agggcatgca
     3181 cactctccgg cggcgagtgt agcgccatgc tccgaagatg ttcctgcaac agttcatgta
     3241 tctcggagcc tctgagatcg tctatttcga tttgcatgag aaactccact tattgagtgg
     3301 cgaattttca tcacacaacg ccgaacgccg ggccatgtgg cgtgccggga atggtcagcc
     3361 tgttcgcctg ctacgtgccg ccgtcatgct actttcttcc agatgatcga atagcgccac
     3421 agtaaatgtt tcctcacctc cgcgccggga agcatgtccc cacaaacctg atggacctga
     3481 gccatggtga gataagaatc gtgccgcccg tgctcagccc atgcttcgcg aacatggcgg
     3541 ggcgccatca cccgaccgtg cctgatcagc ttcaatccca tactcaccgg cgccgccaat
     3601 gcgctggtga gcgcgtcaac cagtccctca ttttgaaaca aatcgagaat cagtagaacc
     3661 ccgttgacct tcagggcgct cttaatcttc ggcagcacct ccgtcatggg cagatgatgt
     3721 agagtggcga ccgtcgcaat acaatcgaac ctttcagccg gaagctctcg cgtcatcacg
     3781 tcggcgacct gaaaatcaat gttcggaaac gacgctgagc gttctctggc aatgcggatc
     3841 atgttcggcg atagatcgag tgcgagcacg cgctctgaag acttcgccag aaggcgtgag
     3901 aagacgcccg cgccgcagcc gatgtccagc gactcccggc agggtgacgg gacacggcgc
     3961 aacaggaaat catggtattg gctgctgtga tcccactctt cgccggagag cagcgcgagg
     4021 cgatcaaaat ctgtccgaat ggtagacatt gtttcagcca tagaggccat ctaacgctgg
     4081 aattgacccg ccgcggcgcc agtccacgca cacgaaccat gctactggtc tgctcgatac
     4141 acatccgtac tcatgtatct cagaccggaa tccaccatca gtgtgaccac tgtagcctcg
     4201 gggccgagac gttcggcgac gcgaagggcg gcgagaacgt tgaggccgga tgaagtgccg
     4261 gcgaagatgc cctcttcgcg cgctaagcgg cggcacatct gtatagcttc ttccgtcgag
     4321 tccgtcagga tttcatcaac ctcgtccggc ttccaatgcg gcgggatgaa gccgatgccg
     4381 atgccctcga tcttgtgtga gccgggcgac ttgccggaca acaccgccgt ttcggacggc
     4441 tccacggcgg cgacatggag ttcaggcttg tgttgacgca gagctttcgc cgctccgtga
     4501 agggagtgag cggagcctac cgaatggaca aaggcgtcaa cctctccgtc tgtttgctcc
     4561 cagatttctt cacccatcga ataatagccg gcggcggcat cttgattact aatctggtta
     4621 acccaccagt gtccgggttg ctgactgatg cggtgggcgg tgtcgatcat gtctttaatc
     4681 agcttctcgt taatcttctt gttgtcgctc ggaagacagg ttacctgagc gccgaatgcc
     4741 tgcatggttc gtatcttttc ttcgctgaag gcgtccgaag tgacgatctg aagacgatag
     4801 cctttcgccg cgcaaaccat cgccagcgag attcccgttg agccgcccgt gtactcgacg
     4861 acggcgccgc cgggttgcaa gcgcccgctc gcttccgccg cttcgataac ggctttggcc
     4921 atcctgtctt tcatactgcc ggtcggattc gcccattcca actttgctaa aacgcgtgcg
     4981 gagccgggcg gcacaatctt ccgaagcggg acaatcggcg tgtcgccaat ggagtcgaga
     5041 atgttagaag caatgttcat aacctttaaa gccgtgaatg gtaaatagta aatcgtgaat
     5101 cgtgcttgag gcaggatcaa cttgaatggc gagtcgcagg tcggagtatg ggcaatcgat
     5161 tgacgattta ccattcacga tttacgtcct ctgcatctcc cgatgcagcc acgccttcgc
     5221 gcgccgccac tcgcgcgtga ccgtgtcgga ggagaccttc aacacctcgg ccgccgcttc
     5281 gacggggagg ccgccgaagt agcgcgaact ccacgacacg gccttttgcg ctcgtcgatc
     5341 tccgagagcg cggcgagcgc cgaacggtcg ccgtccgacc acgcggccag cagctccttc
     5401 atgctcggcg gcgagtcggg ggacgattcc tcagaatcac tcacttcatc tttcatccct
     5461 catccctcat ccctcgcccc tcatccctgc ctcttccagt cgcccgactt gccgccggtc
     5521 ttctcttcga gacgcacgtc ggtgatctgg attcccttgt cgagcgcctt gcacatgtcg
     5581 tagacggtga gcgcggcgac cgcggcggcc gtcagcgcct ccatctccac gccggtctgc
     5641 gcgacggtgg acgcttcggc ttcgatgtgg acgccgtcgt ctcgaatctc ggcgcgcacg
     5701 tcggcgtgcg tgagcgggag cgtgtggcag agggggatga ggtcggccgt gcgcttggcg
     5761 gccatgatgc cggcgaggcg ggccgtctcc agcgggtcgc ccttcggagt gcgccgctcg
     5821 cgcagggccg ccacggtctc cggcgacatc aagacgcgcg ccgaggcgcg tgcgcgccgc
     5881 gcgctcagtg ccttcgcgcc cgtgtccacc atccgcaggc gtccctcttc gtccacgtga
     5941 gacagctcgc tcatggggtg agagtaaaac cgattagacg tgcgcgaagc aagccctgcc
     6001 gcacgcggcg cgcgccggtc tttttttacg caagaatgtc tggcgaaagt gtgtctggaa
     6061 aaaaggtgag gcggcgcaat ccgcgcgcgt gtcgtcccgc gtaattctgg caggctaagg
     6121 aatccaaaac tgagaaaggt ttaagcaatg aaaaaactac tgttcgctat ggtcatgacg
     6181 tgcgtcggcc tcggcatctc cgccgcggaa gtttcggcgc agcagaatcc gatggttggc
     6241 ggagcggcga tgtacaagag caagaacatc gtcgagaacg ctttgaactc gccggatcac
     6301 acgacgctcg tcgccgccgt caaggccgcc ggcctcgtcg agacgctcgc gggcaagggg
     6361 ccgttcacgg tcttcgctcc ggtcaactcc gctttcgaca agctccccgc cgggacggtc
     6421 gaaacgctgc tcaagcccga gaacaaggat atgctcacgc aaatcctgac ctaccacgtc
     6481 gtccccggca aaatggacgc caagaagatc atgagcgcca tcaagaaggg cggcggcacg
     6541 gcctctttca agaccgtcag cggcaacacc ctgacggcgt cgatgagcgg taacatggtt
     6601 gtgctgcgcg acgagaaggg cgggatgtcc accgtcacca tcgccaacgt gatgcagtcg
     6661 aacggcgtca tccacgtcgt tgacaccgtg ctcatgcccg gcgagaagat gatgatgtcc
     6721 gacggtatga agaagacgat gtaacgtctc ttcgagacaa aaaaattcaa ggggcgcctc
     6781 gttcaaaaga cggggcgccc ctcattattt ttcggcgaga ctgttttgcg tgcgccctcc
     6841 ctcacggtcg ggcttgcggc cccggatgtg cctcaaggcg gaagcccgac cgtgagggag
     6901 ggcgttcaga tgccacactc aaatgtcatc cataataaac gcgccgtccg tcaaacgggc
     6961 aggcgcacta gctggacgac gcttccggcc tcgacctcac gaacatccgc ggcgacgatg
     7021 acgagcgcct cggcgcgcgc gaaggcgacg aagtcggacg agccgcccca cttcagcggc
     7081 tccgccatga gccggccatg ttcgtctgtg aacaagcgcg cgggcaggta actcgttctc
     7141 tcgaccgtgc cgcgcgtccg gcgcgagagc accccgcgct cctctttgag catgggttcg
     7201 agcgcgccct gcatcgcgcg cagcgccgtg cgcgcgaaca ggttgaaggt gacggagacc
     7261 gagacgggat tgccgggcag cccgaacagg agcgtctgct tcgcgcccgg caggcgcgcg
     7321 aagacgctcg gcttgcccgg ccgcagcgcg acgcgctcga agaagacctc cgcgccgagg
     7381 gcgcgcatcg acgccttcgt gaaatcgtag cgccccatcg agacgccgcc cgagagcacg
     7441 accacgtcgg agcgcgtcga tgcgtcggcg atcagtcttt gcagcagctc cggctcgtcg
     7501 cccgcgaagg gcatctgctc caccaccgcg ccggcgagcg cggcgtaggc ggcgagcgag
     7561 tgagtgttcg agtcgcgaat ctggtcggcg ccgggccgct cgtccacgcg gacaagctcg
     7621 gcgcccgtcg cgagcacggc cacgcggggg cgtcttcgca cggacacctc ggcgtagccg
     7681 aacgaggcga gggcggccac gctcgccgca cccacctcct cgcccgcgcg caacaccacc
     7741 gcgccctttt tgatctcact cgcgcgcggg acgaagaact gccccggcac ggtcgcgcgc
     7801 atgatctcga cttcgtcgcc gccctctgac tcgcgcgcga gctcgacctg ctgcacgccg
     7861 tcggctcccg tgggcagcgg cgcgcccgtc atgatacgca cggcctcgcc cgcccgaagc
     7921 gtcccacgcc agccctgccc ggccgccgcc tcgcccacga cgcgcagacg cgccgggagt
     7981 tgtgcaaggt ctgtcgagcg cacggcgtag ccgtccatct gcgcgcggtc gaacggcggc
     8041 aagtccgtgt cggccacgac cggctcggcc agcacgcggc ccggcgcttc gcgcagcgcg
     8101 acgcgctcgg cgtcgagggc ttctgtcttc tccagaacga tgcggacggc ttcggcgacg
     8161 ggaatcataa gcggcgggaa ttgtagcaga gcgggaaagc atggacgcct gagccgcaac
     8221 gcgcgcgccg cggctcaggc gtccgcgggc ggtgcttcag aaagcgcgcg ctccgcgggc
     8281 gcggagttgc ggcggaagcc ttcaaagagg atgccgccga attgctccaa cacttcctcg
     8341 acgagcgtga tgacgggcgc gggttgcttc tccacgaaga gggcctcgaa ggcgttgccg
     8401 aaacgttcgg cgaagccggc gtccacttcc cgcagacgac gtgggatagt cttgctgtgc
     8461 gccgtcccga gattttggga gagcaggaag aagtcggcga ggtcggcgta gagaaacgtg
     8521 cccgaggcga cgagttcttc gggcgtgcgc gggtagcgta tgtcgtccac ccagtcgctc
     8581 aggcggtagc gccggcgcat cagctcggcc gcgtcccagc gcggcggccc ggcctccaaa
     8641 agctccgccg cgctgcgctt caccccggcg gagaactcgc tcgccccagg tatctcgatg
     8701 ccttcggtca ccatgcgcat gagcgagggg atgccgcgcc gccggtcgga ctcgaaatgg
     8761 tagcgcagcg tctcgggatc atggacgaag gtctcgacgg gccagcccgc gtggaagaac
     8821 gcctcgcggt aggcgtgggg caggcgctcg tagacaacga cgaggtcgag gtcggaagcc
     8881 ggcgtcgcct ccccgcgcac gaccgacccg gcgagaaaga tgacgcgcgc gccgtcgtac
     8941 ctctcggcga aaattgcgcg cgcggcccgt tcgggtttga ttctttccgt atgcatcaag
     9001 tgtccaacga ctgaacatct ccacagaaat ttttgccact gatactcttg cataggaagg
     9061 cgagagagtt ccagcacggg atgctcgtct ctcgccctct aacgccggaa ttgagccgcc
     9121 gcgtatgcga acagtaccgc gagcggaagc ctcgcgggtc acgcgcatgc gcggcgggcc
     9181 tctcatcgta ggccgcaccc gcgaggcttc cgctcgcggt actgtacttc taacagcggg
     9241 tttttgaagt cacggcgcgc ccagccgctt gacgacttca aaaacctgta gcttgtactt
     9301 cagtatcgga tggcgtaaat tactctccta atatttacga gttgccgcct cagcgacggg
     9361 cgttcgccgt gggaacggcc gggcgacatt caaacctccc cctctaaccc gggcagcttc
     9421 gtcccggtag cctcctccgg catgggttcc tgctcgcaca gtgacccggt taataatctt
     9481 tctcccgctg ccgcctcaat cgccaagcag cggtgcatac ccccgacagg tatgacaagg
     9541 agatcagacc tcagactcat cgtcaccttc gccctcctca cgttcttact tacggcgggg
     9601 gtgattctgg cgtgggaaaa tttgctcctc ccgcccttct attcgtgggt tgacgcgcgc
     9661 taccccggcg aggccaacgt ccagacgcgc tggaacatcc agcagcgggt cgaacacttt
     9721 ttcatctcga tcacggtaga cgtggtggtc gtgacgttgc tggtcggcat gttgcgcgct
     9781 cagcaacgcc ggctggcgca aagcgaaggg cgctaccggt cgctcttcga gcacgcccgc
     9841 gacggcatcg gcgtcgtcca cgcggggagc cgccggctga tcgaggcgaa ctccaaattc
     9901 tgcgaagtgc tcggttgtaa gccgccagag gtaatcgggc gtaagctcga cgagttcctt
     9961 cgcacgccag acgctccgcg gcctcccctc atctcggagt tgcttgacag cgggaagtcg
    10021 ggcgagggcg agatgtccat cctgacggcc gggggcgctt ccttgcccgt ctccttctcc
    10081 ttccactcgc tctcgaccga gggcgaggaa gaactgctca ccctgatcat ccgcgacctg
    10141 tcgtcgcgca agcggctgga ggcggaaaac gaggagattc agcggcagct cttccagtcg
    10201 tcgaagctgg cctcgctcgg cgagctgtcg gcgggcgtgg cgcacgagat caacaacccg
    10261 ctcaacggca tcatcaactt cgcgcagttg ctcaaggacg agccggcgcc gcgttcggag
    10321 tttgagcagc agatgatcga cggcgtcatc gacgaggggg agcgcatcgc gcagatcgtg
    10381 cgggggctgc tgaccttcgc ccgcaacgac tcgcaggaac tgcaccgcgt ccgccttgag
    10441 gacgcggtca aggcatccgt cgcgctcttc ggccgccaac tggagaagga cgggattcgg
    10501 gtcgaggtcg aattcgcggc cgatctgccg ccgatgcgcg ccgacgcttc gcgcctgcgt
    10561 caggttgtcg tcaacctcat cagcaacgcc catcacgccc tccgtgagaa gccgccccgt
    10621 gccggcgagc agaaggtctt tcgcatctcg gcgcggcgcg tcgtcgcggc cgccggcgta
    10681 gtcgtccgca cggagttcta cgacaacggc gccggcatca ggcgtgagga tttgggcaaa
    10741 gtctttgacc cgttttttac gacccgccgc gaaaccgggg gcacgggcct cggcctgagc
    10801 atcaccttcg ggatcatccg cgaccacggc gggacgatca cggtcgcgag cgaagagggg
    10861 aagttcacgc gcttcacgat ggacttgccc gcggctggag cttacgagat cgagggggtc
    10921 gagtatgttt agggtgctgc tggtcgatga cgagccgagt ctgcgcttta cgatgtcgga
    10981 attcctgaag cgggacggct acgaggtgcg cgccgtctcc gactacgccg aggcggcggc
    11041 cgttgggaac gagcacgtgg acgtggcggt cgtggacgtt aacctgccgg gcgggagcgg
    11101 cgttgacctc ctgcggcagt tcagcggtcg cgagccttac gtccccgtca tcatgatcac
    11161 cggcgagccg acgctctcac tcctgcccga catcgtgcgg gccggcgctt acgacttcat
    11221 cgccaagccc gtggtcaagg acgtgttgct cagggccgtc gcgcgcgcca gcgagaagaa
    11281 gcggctgacg gaagagaagg ggcggcttga gcaggaggtc aggcggcacg ccgaggaatt
    11341 ggagacgcgg gtcgccgagc gcacggcgga actcctcgcg gcgcacacgc ggctcgaccg
    11401 ccaggagaaa atcgcctccc tcggccgcat cgcggcgcag gtggcccacg aagtgaagaa
    11461 cccgctcgcg gggctgctgc tttactcgat gcacctcaga gacagggtcg ccgccaagct
    11521 gcccgatgac gagttgaggc tcatcgacaa gatcatcgag acgactcgtt acatggatcg
    11581 cacgtgcgaa caagtcctcg acttcgcgcg ccccatcagc ctcacgccgc tgcccgccga
    11641 cctcaacctc atcgtcgccg acgtgctgca catacttgag ccgcaactgg cggccggggc
    11701 gattgagaga cgtctggaac tcggcgagcc ggaagtcgcg ggcgtgctcg accagacctc
    11761 catccgcggc gccctgacga acctcatcct caacgccgtg caggccatgc cctcgggcgg
    11821 cacgctcgcc gtcaagacct tgagggcgga cggcgcgctc aaggtggaag tctccgacac
    11881 gggcgtcggc atgaacgagg aacagttggg aaacatcttc gaacccttct actcgaccaa
    11941 gagccggggc ctcgggctgg gcatgcctta cgtcaggaaa gtcgtcgagg agcaccgggg
    12001 gacgattcaa gtcgagagcc gcccatcggc ggggacgacc atccgggtct ccctgccggt
    12061 gggcgcggag ggagtttcag catgagccgc caacgaattc tcgtggttga tgacgaggcg
    12121 agcgtcgcgg gcgcgctgga gctgatcctc ggcgagcagg gttacgaggt gagtacggcg
    12181 ccgacggtgc gcgaggccga ggcgctgctc gcgggccgcc ccttcgacct cgcgttcgtt
    12241 gacctgcgcc tgcccgacga cgacggcatc ggcctcctcc aacgcatcag gggcgacgcg
    12301 cccgatacgc aagtgatcct gatgagcgcg cacggctcgc tcgacatcac catcgaggcc
    12361 atcaagcgcg gggccttcta ttacgtcgag aagcccttca cgcccgaaca ggtggtgatg
    12421 ttggcgaagc gcgcgctcca gttcgaggag attagacgtg agaaccggct actgaagagc
    12481 gcgctcgggg gcgacggcga cgacttcggc ctcgtcggca atcacgcgaa gatacgacag
    12541 gtgcgcgccg ccatccgcac cgccgcgccg tctgacgcct ccgtcctcat cgagggcgag
    12601 tcgggcacgg gcaaggagct gatcgcggcg gccctgcaca gccagagcgc gcgctctcgc
    12661 ggccccttca tccgcatcaa ctgcgccgcc atcccctcgg agctaatcga gtcggagctg
    12721 ttcggctacc gccgcggcgc tttcaccggg gcggatcgcg acaagcgggg cttagttgag
    12781 gccgcggcgg gcggcaccct gctcatggac gagatcggcg agatgcccgt ctatctccag
    12841 acgaagctgc tgcgcgtcct tcaggagcgc agggtgcgcc gtctcggcga cgagcaggag
    12901 ttcgacgtgg acttccgcct cgtctcggcg accaaccgcg acacggcgca gatggtcgcc
    12961 gagggaacgc tacgcaaaga cctctacttc cgcatcagca ccgtccgcat caagatgccg
    13021 cccctgcgcg agcgcctcga cgacctcgtc gtgctggccg aacggttcct gctgcgctac
    13081 gccgagaagt atcagaaacg cctgcgcggc atctcgaccg cggctctctc gctgcttacg
    13141 cgctacgact ggccgggcaa cgtccgcgag ctggagtcgg ccgtcgagca cgccgtcctc
    13201 ttcgcgcagg aagagcagat catgcccgaa gacctgcccg agcagctcca cgacgtgcgc
    13261 gccgccagct tccgctgcgt catcccgccc tatgtgacga tggaagagat cgaacgcgaa
    13321 gccatcgcgc agacgctcga acgcaccggg ggaaacgtca agaagaccgc gcaaatcctc
    13381 agctaccacc gcccgacgct ctaccgcaag ctgaagaagt tcggcctcct gcgcgacgac
    13441 ctgactcacg cgcaggcgga cgtagcccgc ggggagtaga ccggtctact ttccgggcgc
    13501 gccctccatc ccacaaatca ccacacgcgg catgctcgcc cgaatgggca cgacggttgc
    13561 cactgttcat tcgtcgccgg aaggcgggcg agacggggaa acacgcattg acgggtgtgc
    13621 gattactccc cccgcggaca tccaggcacg tgatgggaaa aacggaaata ctgatcatcg
    13681 acgacgagcg gagcgtggcc gacgctctca aggtgatcct tgagagcgaa ggcttcgacg
    13741 tgacgctggc ggcgagcggg cgcgacggta tcgagcgcgc aaggcaggaa aaatttcgcc
    13801 tggcgattac cgacttgcgc ctcccggaca tgtcgggtct ggaagttttg cagacactcc
    13861 gggcggaaaa ccccggcctc cgcgtcatcc tcaccacctc ctacggcacg ccgaaaatcc
    13921 tggccgaggc gatagggttt ggcgcggagg gcacactgga taaacccttc gccccttcgg
    13981 aaatcgttcg cctgataacc tccgtgcttg agcgcacgcc cgacgctcgg tcgtgatcgg
    14041 gcgtcgggcc gcgcgccgcg ctcccgagct gatgaacgct cgtcgccgac cgcccctcag
    14101 ccatgctctc gacgacgggc gcgtcagcgt gtgccgggtg attcgcactg aggcaatagc
    14161 agcggcccgt gtttcacggt aaaacacact cgccggaata gtcccgccgc cacgctcgga
    14221 aacgcccgcg attacagtcg ccaggaaaaa aaatatcagg catgatggtt gctctaaggt
    14281 agcttgctgt tatcagtcga aggccgtcgg cgtcaccctt ctccctcaag cggcgagttc
    14341 gaagcaagta cgcggcgacc accggacgta accagcgaag ccgggccatc gccgtgcgcg
    14401 ccgctcaaga ggagtgcagc ggtcggcccc tacacacgct cgccctcgcc gcccgacaat
    14461 cacgaaccgt ctctgataga accacaacga tgaagaagag aagccacata ctgctcaccg
    14521 cggcgctgct cctttgctcg ccggcactca cttccaccac gacgcgcgcg caggaggaca
    14581 agacggcccc cgacgcgaag gcgtcagttc agaaggacgc gaaagtcgct cccgtggcga
    14641 cgcccgcgcc cgtgcgcggc ccgcagaaga tcgtgaagga tggggtcgaa gtcgagttca
    14701 cgattgagcc ggtgacgccg tcgggcgctt cgcccgaact gttggccggg caggacgccg
    14761 tcgtgcgctt ccgcgtccgc gacacggcga ccggaactcc gctctccggc gtcaggccct
    14821 cggcctggat caaccggcgc accgggtcgg cgccgcccga cgcccagcag tgccgcgagc
    14881 tgatccagtc gtacctgcaa ggctccctgc gggcgcgccc cgacgttgac ctcaacgcct
    14941 actacatcct cgccctcaac gaagagccga acctctcagt catcgacccg ctgctcggct
    15001 tcggcggcag taagctcgtc acgctcgttt tcctcaagag tcccggcgcc gactgggcgc
    15061 tcaccgccga ccgctcgcgt cttttcgtca gcatgcccgc ggccggcctg gtagccgtcg
    15121 tggacacgac gacgtggaag gtcgtcgcgg acgtgagcgc gggcgcgagg ccgacgcgcg
    15181 tccgcttgca gccggacggg aagtatctat gggtcgccaa cgacgcggag ggagcgggcg
    15241 cgtcgggcgg cgtcacggtc atcgacacgg cgagcttgaa agtcgcggcg agcattccca
    15301 cgggcgcggg cacgcatgac ctcgccttct cggaggacaa ccgcgcggcc ttcgtcacca
    15361 accgcgacga gggcacgctc tcaatcatcg acgtgcggaa gctcgaaaag gtgaaggaca
    15421 tcaagaccgg cgcgcccgcc tcggcgctcg ccttctcgcc gctgagcaag gcgctctacg
    15481 tggccaacga gacggacggg agtctcagtg tcgttgacgg ccagagctac gagttgcgtg
    15541 cgcgcatcgc ggcgcagccc ggtctgcgct cggtgcgctt cgcgcccgga ggccgctggg
    15601 gcttcgtccc gaacgcaacc cgctcggtcg tccacatctt cgacgcctcc accaatcgcc
    15661 tcgtccacga cgttcccgta ggcaagacgc ccgatcaggt cgccttcacc gacgctttcg
    15721 cctatgtccg ctcgctcggc gccgaggagg tgtcggcgat ccgcctgagc acggtcggca
    15781 aggagccgga cgtggtgaag ttccccggcg ggcaggccgc gccgggcacg tcgcgcagcg
    15841 aggcgctgag cgccgacgcc ttcgtccccg cgcccgaaag cagcaccatg ctcctcgcca
    15901 acccggccga ccggatgatt tactactact ccgagggcat ggccgccccg atgggcaact
    15961 ttcagaacta caagcgcgag ccgcgcgcca tcaaggtcgt tgaccgaagc ttgcgcgagg
    16021 agtcgctggg caactatgcg acgaccctgc ggctgccggc cgacggaatt tataacgtct
    16081 ctctcctgat ggattcgccg cgcgtcgtcc actgtttcga ggcgacggcc aaaccgaacc
    16141 cggcgatgaa gcaggagcag aagaccgcgc tccgcgtcga atatctcaac aaggaacagc
    16201 cggcgctccg tcccggcgcg agctacaaaa tccgcttccg gctgacagac gcggctacgg
    16261 ggcagccgaa ggaagacctg aaggacgtgc gcgtgctgtt cttcctctcc cccggcatct
    16321 ggcagactcg cgacttcgcg cgctccgtag gcaaagggat ttacgagctg gaggtcaccc
    16381 ccccgcagga gggcgtctac atgatcttcg tcgagagccg gtcgcagggg gtttcgttca
    16441 ggcatttgcc ccacctcacc ttgtcggcgg acgccgcgcc gcccggaagc tcacagtgac
    16501 cgcggcccgt tgacttcaga cgaggtaggc cgtgcgagcc tgatggtgaa ctgcaaagtt
    16561 ccggcgacgc ccggcaagag tgtggacttc ggaggaagga aagatgaaca gtaagacttt
    16621 atcttcgttc gcgcgtcgcg cgtcgctcgt gctggcggcg gcgctcgtgg ccggcgcgag
    16681 cgacgcgccc acggttcgcg cgcagaagaa gaagaagccg cagcccgcgc gggtcgagtc
    16741 gccggaggcg tccgcgcact actcgtgccc gatgcacccg tcgatcaaat ccgcgaaacc
    16801 cggcaggtgc cccaagtgca agatggagct gcgtctgacg aaagacgagc cagccgaggc
    16861 gaagaccgtg attgcgaaga cgccgacgcc ggccgcatcc gactcggtgt ccggcatgaa
    16921 catgcccgac accgagctac tcgatcagga cgggcgcaag gtgcgcttct acaccgacct
    16981 cgtcaaagac aagaccgtcg tcatcaattt catcttcacc acctgcacga ccgtctgccc
    17041 gccgctcggg gcgacgtttg cgcgcgtgca gcgcgagttg ggagagcgca cggggcgcga
    17101 cgtgtacttc atctcggtgt cggttgaccc ggtgacggac acgcccgagc gtctgaaggc
    17161 gtggggcgcg aagttcaagg cggggccggg gtggacgttt gtgacgggag cgaagccgcg
    17221 ggtgaacgag gtgctcgccg cgctctccgc ctcgtccgca agccgcgaag accacacgcc
    17281 gaccatcctc atcggcaacg acgcgaagcg gcagtggacg cgcgcctacg ggctgaccgc
    17341 gccgagcaaa atcgtccaga ttatcaacga cgcggccagt ggccgcctcg catcgaacac
    17401 gaaatagtcg aagccgccga aacagtcaaa gtaatcgaag gagccgaagc aatgataaag
    17461 agagttctac tcgtcgccgc cgtttgcctg tcgctcgcct ccacggccgc ggcgcaacag
    17521 acggccaagc cgcccgcccc gccgcccgac ccgcacgcgg gccacggcgc gcccgcgcga
    17581 gccgagcagg agccgaccgt cgccgcgaaa tatttcacgg acgtggagct tgtgaatcag
    17641 gacggccaga aggtgcgttt ctactcggac gtgctgaagg gcaagaccgt cgtcgtcaac
    17701 gccttcttca ccacctgcac ctccgtctgc ccgccgatga atcgcagcat ggagaagatt
    17761 caggaggcgt tcggcgaccg cgtggggaag gacgttttcc tcgtctccct cacggtcgat
    17821 cccgcgaccg acacgcccgt gcgactcaag gagtacgccg cgaagttcca cgccgggcag
    17881 ggctggatgt tcctcacggg caagaaggag aatctcgact gggcgctcta taagctcggc
    17941 cagtacgtcg agaacaagga cgaccacaag acggtcatca tcatcggcaa cgaggcgacc
    18001 ggcctctgga agaaggcgtt cgggatggcg aaggttgagg agctaatcaa gctcgtcaaa
    18061 gaggtggtcg atgacaaggg tgatggcaag acttcggccg cccagtaaaa actcccgacc
    18121 gccaacgagt ccgcgcttcg gcatgcttca aagtctcaaa ctcacgatca cccttctcgc
    18181 tctggcgttc ttcgccgcct tcacactcct cggcgctccg gccggagctg agtcgtgggc
    18241 cgtgcagggc acgacgacga cgacggcgag gccgcagccg ctcaccgagc aggagcggcg
    18301 cgggcgctcc ctctacctgc gcggcgagag cgcgtcgggg agcgagatcg tggcggtagt
    18361 gggcgagctg gacgtgccgg cctcgaccat gacctgcgcg ggctgccacg gcgcgcgggg
    18421 cgagggcaag accgagggcg gcgtgacggc cggcagcctc gtctggtcgc acctcgtgaa
    18481 gccttacggc cacatgcacc cgtcggggcg caagcacgcc aatcccttca ccgagtcatc
    18541 gttcatccgc gccgtctcaa acggcgtaga cccggaaggc aacgacctgc tggcggcgat
    18601 gccgcgctat cggatgtcgg ccgccgacat ggccgacctc atcgcctacc tcaagcgcat
    18661 cgagaccgac ctcgaccccg gtctctccga cacgagcctc cgtgtcggcg tcgtcctccc
    18721 ggccaaaggc gtgctggccg agacgggcgc ggcgatgaaa gacgtgctcg cggcctactt
    18781 cgacggcgtc aacagtcgcg gcggggtcta caaccgaaag atcgagctgc gcgtcgccga
    18841 ggcgggagac gacggcgcgg cgacggccgc cgccgcgcgc gccttcgcgc gacaggagca
    18901 ggtcttcgcc ttcgtcggcg gcatcagcgt cggcgcggac gccgagctgg cggcgctggc
    18961 gcgcgacgag gagattcctt tcatcgcgcc ctcgacgctc acgccgcaga ccgggactcc
    19021 gcccaaccgt tacgtctttt acctcctgtc gggcgtcgcc gaacaggcgc gcgctctggt
    19081 caacttcgcg gcgtcgcaaa aggagttgaa taagaagcgc gccgcaatcg tctacacgga
    19141 gggcacgctc gccgtcgcgg ccgccgcggc cacggaagat caggcgaaga agtccggctg
    19201 ggccggagtc acaaagcaag ggtacaaagc agacggcttc gacgccgcgc agatcgtccg
    19261 gcggctgaag caggaggggg ccgagtcggt cttcgtcttc ggtggcgagg cgtcggcgct
    19321 cgtcaaagag gccgcggcgc tgggctggac gccgcacgtt ttcttcctcg gtatgatggc
    19381 cgggagagac ttccccggcg aggcgccggc cggcttcaag gacagggttt ttctctcctt
    19441 cccgaccatc ccggccgacg tgaccccggc cggcgtggcc gagtttcgcg cgctgcaaga
    19501 gaagtacaaa ttcgctccgc gccacgtggc ggcgcagctc tcggccttcg ccgccgccaa
    19561 aatcttcgtc gaggggctac agcgcgtcgg gcgcgacctg agccgcgaga ggctcgtcac
    19621 ggcgctcgaa ggcttctacg acttcgagac gggcgtcacc ccgcgtctta ccttcgggcc
    19681 gaaccggcgg gtcggggcgg cgggcgctta tgtcgtgacg attgaccctg agaaaaaaga
    19741 gttcgtgccg gcgagcggat gggtcaaggc ttactgaaac ggaatagaag gctctccgcc
    19801 ggaatcactt catcgaccgc acttcatcga ccgtacttca tcgaccgttt agaggcggac
    19861 acaccccgcc tctaaacggt cgtgggttat ggtcatccgc aaatcgcaac cgtgctcgat
    19921 aagcccgcct ccaaaccgta accaataagc tcgaacgaaa cccgcttcag ttcaacgacg
    19981 gacgacgcgc gcgccccaac ccggcgacgg acgtgtgcgc gcgcccgcct cgtctcaccc
    20041 gcgacgtgat acatggtgaa acactacgcg ctccctcgtg ccgcacgctg aaacatcccc
    20101 gccgagatgt tcaacctcga aacttcataa tcacatagtt attgaaccgt aatgaacagg
    20161 gcacggcgct tgctaatcgc aacac
//
LOCUS       DASVES010000360         6104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1026265, whole genome shotgun sequence.
ACCESSION   DASVES010000360 DASVES010000000
VERSION     DASVES010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026265"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VF240_14685"
     CDS             <1..396
                     /locus_tag="VF240_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176509.1"
                     /translation="CDNTKSNLGTVQFRRKYTNNTGGPITRLRFRITEITTLNNRLAT
                     EADYRALHNTGNILVSTSGGNVTVESLQLEPPSDAVTNGGALNSTLAAGTITTMTPLA
                     NGSHINVNFLFGVQQIGDYRVFITIEALP"
     gene            699..1913
                     /locus_tag="VF240_14690"
     CDS             699..1913
                     /locus_tag="VF240_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HEX7176510.1"
                     /translation="MPASELIYDWNTVEPQMAAPGRPISFDDETLRDGLQSPSVAEPP
                     VEQKIELLHLMDALQIDTADIGLPGAGGTHAAGVERLAREIVEQKLRIRPNCAARTHQ
                     NDIRPIAEISQRAGIAIEACTFIGSSPIRFYAEEWTLDKLLRLTEDAVTFAVSEGLPV
                     MFVTEDTTRADPETVRALYTAAIRAGARAVCVCDTVGHATPDGARSVVRFVKRIIEDE
                     GGGVRLDWHGHQDRGLGVINSIAAIEGGADQVHGSALGVGERVGNTPMDQLLVNLKLM
                     GWITNDLSRLREYCDKAAEACQWSIPVNYPVFGRDAFRTATGVHAAAVIKSFRKGDDE
                     LADLIYSGVPAGLFGMRQTIEIGPMSGKSNVIYWLESRGLDPADERVQRIFDHAKSAH
                     GVLSEDEVMGLV"
     gene            2053..2451
                     /locus_tag="VF240_14695"
     CDS             2053..2451
                     /locus_tag="VF240_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7176511.1"
                     /translation="MRKNISSGTRWEPLVGYSRAVRVGDVVHVSGTTATDADGNIVGA
                     GDAYAQAVQTLRNIERALTAAGASLRDVVRTRMYVRDIERDWESVGRAHGEFFGDVRP
                     ATSMVEVSRLIDPEMLVEIEADAHVNAADS"
     gene            2545..3780
                     /locus_tag="VF240_14700"
     CDS             2545..3780
                     /locus_tag="VF240_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7176512.1"
                     /translation="MNTSAAAVSAERVVSDLVADARVRRAFRLFEKCAAEIDAEHVRI
                     CEVPAPPFGEEERAKFLAEKFRNSGLADAGLDEEGNCVALRAGKSLRPLLIVSAHLDT
                     VFPAGTDLTVRRDGAKMRAPGVGDDGCGLVALVALLRALEDCSIETEGSLLFVGTVGE
                     EGEGNLRGVRHLLTAGEWAGQVDAFLSLDGPGVERITNAAVGSRRHRVLMRGTGGHSW
                     GDFGAANPVHALGRAVARLAAYPAPPSPRTTFNVGRVRGGEGVNVIPREAEMDVDLRS
                     ESADELARLDAFFRRAAREAADEENAARRPGTPPLELEVKLIGDRPGGVTPPTSPLVR
                     LAEEATRAVGSRPQLDCSSTDSNLPISLGVPALTLGSGGASGNSHTHEEWYDPRGRDL
                     GLKRALLVILGTIGLAGDA"
     gene            complement(3942..4988)
                     /locus_tag="VF240_14705"
     CDS             complement(3942..4988)
                     /locus_tag="VF240_14705"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7176513.1"
                     /translation="MALTGQLSDMSLAELIEFFCNQRKTGRLKVDYHRGHGVFFIKEG
                     ELVDAKVGQLSGAEAVYFALTMPNAAFDFSPEVPPTRRTISESWTQVVLEGLRRLDEG
                     LSPSESDAFGGWSPSESDMALFMDQVDRLGAATHKSEGQTAESTPLSMMVENAGADGG
                     SRKKLMMAGAAGCIVLAVVIGAIPFLRGSSKTAASATSKDATPPETAAGAPTGDATAN
                     PAAPEAVAPALEVSASADDAALAAQREQREREQRLREQRERERRADETARKTEAAAGA
                     NANSSTPAAAPAGPKSVRVQITYDEAGRVTAASVAGASPGAEAFGGTAVRVARGKRFP
                     AGKAGSTVVTIPVN"
     gene            complement(5045..>6104)
                     /locus_tag="VF240_14710"
     CDS             complement(5045..>6104)
                     /locus_tag="VF240_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7176514.1"
                     /translation="VRRVKTETAKITAKGGKPVEGPRVLLETATYDIKGGKIDNAYFL
                     AASGGTLTGKEVYKYDDKGNIVEMTLQGEDGNLLSKETYTYDFDAFGNWTKMITSVAV
                     IEGGKLSFEPTEVTYRSLSYFVDEATLARMSQPAAAPAAATSVPAAATSAPAPAAASP
                     DKQPASVPADNKTAATPLKSNAAPASAERADAKGVKTDGARMAAAPPPMVSTDRTGAA
                     GSSPMASVVRTGSSSAGPVVNSEGEAPARETPERVIARGPVKPVSGGILNGKAMTLPM
                     PLYPEMARRAHKMGVVIVEVIVDVTGRVISAKAVEGPTMLQAAAEAAARQARFSPTLL
                     SGQPVRVSGQITYNFTIK"
BASE COUNT          968 a         2156 c         2005 g          975 t
ORIGIN      
        1 tgcgacaaca cgaagtccaa tctcggcacg gtgcagttca ggaggaagta caccaacaac
       61 acgggcggcc ccatcacgcg cctgcgtttc cgcatcaccg agatcacgac gctcaacaac
      121 cgcctcgcca ccgaggccga ctaccgcgcg ctacacaaca ccggcaatat actggtgagc
      181 accagcggcg ggaacgtcac ggtcgagagt ctccagctcg aaccgccgtc cgacgccgtg
      241 accaacggtg gcgctctcaa ctccaccctc gcggccggca ccatcacaac catgaccccg
      301 ctcgcgaacg ggtctcacat caacgtcaac ttcctgttcg gcgtccaaca gataggcgac
      361 tatcgcgtct tcatcaccat cgaggcgctt ccgtaaaaaa aaggccgcgg cgtcgctagc
      421 cgacgcggcc gtcgccggaa ggcgcgccgc acacccctcg cgagggtgag cggcgcgcct
      481 tccgcttttt tacgaaggag tttccacgcg gctcgcactt cgggcccgcg cgcgtcgaag
      541 aaagccgcgc gatgtgaggc cggtcgcggc acgcggagcc gcgacggcgc gtctgcttga
      601 catgccccat cagccgactg tttaatccaa tttctcctcg gaaaaacgct ggaagaagac
      661 gacaaagatt tcctgccgac agggaggtgc cgctagaagt gcccgcaagt gaactcatct
      721 acgattggaa cacggttgag ccgcagatgg ccgcgccggg gcgccccatc agcttcgacg
      781 acgagacgct gcgcgacggt ctgcaatcgc cctccgtcgc cgagccgccc gtcgagcaga
      841 agatcgagct gctccacctg atggacgctc tccagatcga cacggccgac atcggcctgc
      901 cgggcgcggg cggcacgcac gccgccggcg tcgagcgtct ggcgcgcgag atcgtcgagc
      961 agaaattacg gattcgcccg aactgcgccg cgcgcaccca tcagaacgac atccgaccca
     1021 tcgccgaaat ctcgcagcgc gccggaatcg ccatcgaagc ctgcaccttc atcggctcct
     1081 cgcccatccg cttctacgcc gaggagtgga cgctcgacaa gctcctgcgg ctcacggagg
     1141 acgccgtcac cttcgccgtc tccgaggggc tgcccgtgat gttcgtcacc gaggacacga
     1201 cgcgcgccga cccggagacc gtgcgcgccc tctacacggc ggccatccgc gcgggcgcgc
     1261 gcgccgtctg cgtctgcgac accgtcggcc acgccacgcc cgacggggcg cgctcggtcg
     1321 tccgcttcgt caagcgaatc atcgaagacg agggcggcgg agtccgcctc gactggcacg
     1381 gccatcagga tcggggcttg ggcgtcatca actcgattgc ggcgattgag ggcggggccg
     1441 atcaggttca cggctcggcg ctcggcgtcg gcgagcgcgt gggcaacacg ccgatggatc
     1501 aattgctcgt caacctgaag ctgatgggct ggatcacgaa cgatctgtcg cgcctgcgcg
     1561 aatactgcga caaggccgcg gaggcgtgcc agtggtcgat acccgtcaac tacccggtct
     1621 tcggccgcga cgcctttcgc accgccacgg gcgtccacgc cgcggccgtc atcaaatcct
     1681 tccgcaaggg cgacgacgag ctggccgacc tcatctactc gggcgtcccc gccggtctct
     1741 tcgggatgcg gcagaccatc gagatcgggc cgatgagcgg caagtccaac gtcatctact
     1801 ggctcgaatc gcgcggcctc gaccccgccg acgagcgcgt ccaacgcatc ttcgaccacg
     1861 ccaagagcgc gcacggcgtg ctcagcgaag acgaagtgat ggggctggtg tgaagagagc
     1921 tgtcagctat tagcaattag ctgttagctc aagacaggag catcagcggc gtcgccgacg
     1981 gttatcgaaa ctctatcgtc cgctctaaat tctggctaac ggctgaaagc taacagctaa
     2041 cagcttcctt ttatgcgaaa aaacatttcg agcgggacgc gttgggagcc gttggtcggc
     2101 tactcgcgcg cggtgcgcgt cggcgacgtg gtgcatgtgt cggggacgac ggcgacggat
     2161 gctgacggaa acatcgtcgg cgccggggac gcctacgcgc aggccgttca gaccctgcgt
     2221 aacatcgagc gggcgctcac ggccgccggc gcttcgctgc gcgatgtggt gcggacgcgg
     2281 atgtacgtcc gcgacatcga gcgcgactgg gaatcggtcg ggcgcgcaca cggcgagttc
     2341 ttcggcgacg tgcggcccgc caccagcatg gtcgaggtga gccgcctcat cgaccccgag
     2401 atgctcgtcg agatcgaggc cgacgcgcat gttaacgcgg ccgactcgta agcgcgcgcc
     2461 ggcccggccg acatctgagc gcgcgccgga gcgcgagcgc gcgagtccat tgaagttgcc
     2521 gccgcccgcc cgctcttaac tcctatgaac acttccgccg ccgcagtctc cgccgagcga
     2581 gtcgtctctg atctcgtcgc cgacgcgcgc gtccggcgag ccttccgcct cttcgagaag
     2641 tgcgcggccg agatcgacgc cgagcacgtc cggatctgcg aagtgcccgc cccgccgttc
     2701 ggcgaggagg agcgcgcgaa gtttctcgcg gagaaattcc gcaacagcgg cctcgccgac
     2761 gccggcctcg acgaagaggg caactgcgtc gccctgcgcg cgggaaaaag tttgcgcccg
     2821 ctgctcatcg tgagcgcgca cctcgacacg gtcttcccgg ccggcaccga cctcaccgtc
     2881 cgtcgcgacg gcgcgaagat gcgcgcaccg ggcgtcggcg acgacggctg cggactcgtc
     2941 gccctcgtcg ccctcctgcg cgcgctcgaa gattgttcga tagaaaccga ggggtcgctt
     3001 ttgttcgtcg ggacggtcgg cgaggagggc gaggggaacc tgcgcggcgt gcgccatctg
     3061 ctgacggcgg gcgagtgggc ggggcaggtc gatgcctttt tgagtctgga cggccccggc
     3121 gtcgagcgca tcacgaacgc ggccgttggc tcgcgccgcc atcgcgtcct gatgcgcggc
     3181 acgggcggac actcctgggg cgacttcggc gcggccaacc ccgtccacgc gctgggccgc
     3241 gccgtcgcgc gcctcgcggc ctaccccgcc ccgcccagcc cgcgcacgac cttcaacgtc
     3301 gggcgcgtgc gcggcggcga gggcgtcaac gtcatcccgc gcgaggccga gatggacgtt
     3361 gacctgcgct cggagtcggc cgacgagctg gcgcgtctcg acgccttctt ccgccgcgcc
     3421 gcgcgcgagg cggccgacga ggagaacgcc gcgcgtcggc ccggcacgcc gccgctcgaa
     3481 ctcgaagtga aactcatcgg cgaccgaccc ggcggcgtca cgccgcccac gtctccgctc
     3541 gtccgtctcg ccgaggaagc gacacgcgcc gtcggctcgc gcccgcaact cgactgctcc
     3601 tccaccgact ccaacctccc catctcgctc ggcgtccccg ccctcacact cggcagcggc
     3661 ggcgcctccg gcaactcaca cacgcacgaa gagtggtacg acccgcgcgg ccgcgacctc
     3721 ggcctcaagc gcgccctcct cgtcatcctc ggcacaatcg gtctggccgg cgacgcctga
     3781 caaaactgct ggtcggagga gtctgtctaa aacacgtggg cggcggcctt gtccacaggc
     3841 cgccgcccac gttcccatgt cgtgcggacg cagaagcggc ggcaaataca attgccgccg
     3901 cttccctttc ggggtttaga ctaaagtacg gggccgaccg ctcagttgac cgggatggtg
     3961 accacggtcg agccggcctt gcccgcgggg aagcgcttgc cgcgcgcgac gcggacggcc
     4021 gtcccgccga aggcttcggc gccgggactc gcgcccgcca ccgacgcggc cgtgacgcgg
     4081 cccgcctcgt catatgtaat ctggacgcgc acggacttcg gccccgccgg agccgcggcg
     4141 ggcgtggagg agttggcgtt cgctcccgcc gccgcctcgg tcttgcgtgc ggtctcgtcg
     4201 gccctgcgct cacgctcgcg ctgttcgcgg agcctctgct cacgctcgcg ctgctcgcgc
     4261 tgcgcggcga gcgcggcgtc gtcggcgctg gcactcactt cgagggcggg cgcgacggct
     4321 tcaggagctg cggggttcgc cgtcgcgtcg ccggtcggcg cgccggcagc cgtctccggc
     4381 ggcgtcgcgt ccttcgaggt cgcgctggcc gccgtcttag aagagccgcg caggaagggt
     4441 atcgcaccga tgacgacggc cagcacgatg catccggccg cacccgccat catcaacttc
     4501 ttacgactgc cgccatcggc gcccgcgttc tccaccatca tcgagagcgg cgtggactcg
     4561 gccgtctggc cctcggactt gtgtgtggcc gcgcccaggc gatccacctg atccatgaag
     4621 agcgccatgt cggactccga aggcgaccag ccgccgaagg cgtccgattc gctcggcgac
     4681 agcccctcgt cgagccggcg caggccctct aagacgacct gcgtccacga ctcgctgatc
     4741 gtgcggcgcg tgggcggcac ctcggggctg aagtcgaacg cggcgttggg catggtgagt
     4801 gcgaagtaga cggcctcggc cccgctcaac tgcccgacct tcgcatctac cagctcgccc
     4861 tccttgatga agaagacgcc gtggccgcgg tggtaatcga ccttcagacg gccggtcttg
     4921 cgctggttgc agaagaactc gatcagttcc gccagcgaca tgtcgctgag ttgtccggtc
     4981 agtgccatcg tcagttactc ctcttgagtg tcggggccgc gagtgttggt tgttgtgcgt
     5041 gccgctactt gatcgtgaag ttataagtga tctggccgga gaccctgacg ggctggcccg
     5101 agagcagcgt cggcgagaag cgcgcctgac gcgccgccgc ctccgccgcc gcctgcaaca
     5161 tggttgggcc ttccacggcc ttcgccgaga tcacacgccc cgtcacgtcc actatcacct
     5221 cgacgatgac cacccccatc ttgtgcgcgc gtctggccat ctcgggatag aggggcatgg
     5281 gcagagtcat ggccttgccg ttgaggatgc cgccggagac cggcttgacg gggcctcgcg
     5341 cgatcacccg ctccggggtc tcgcgcgccg gggcttcgcc ctcggagttg actacggggc
     5401 cggcgctcga cgagccggtt ctgacgacgg aagccatcgg cgacgacccg gccgcgcccg
     5461 tcctatcggt cgaaaccatg ggcggcggcg cggcggccat ccgggcgccg tcagtcttca
     5521 ctcccttagc gtcagcacgc tcggccgacg ccggcgcggc attagacttc aaaggggtgg
     5581 ccgcggtctt gttgtcggcc gggacggagg cgggctgctt gtcaggggaa gcggcggccg
     5641 gagccggagc gctcgtcgcg gcggccggca cgctggtcgc ggcggcaggc gccgctgcgg
     5701 gctgcgacat ccgggccagc gtcgcctcat caacaaagta ggagagcgac cggtaggtga
     5761 cttcggtcgg ctcgaacgac agcttgccgc cttcgatgac ggcgaccgac gtgatcatct
     5821 tcgtccagtt gccgaaggcg tcgaagtcgt aggtgtaggt ttccttgctc aggaggtttc
     5881 cgtcttcgcc ttgcagagtc atctcgacga tgttgccctt gtcgtcgtac ttgtagacct
     5941 ctttgcccgt gagcgtcccg ccggaagcgg ccaggaagta cgcgttgtcg atcttgccgc
     6001 ccttgatgtc gtaggtcgcc gtctccagga gcacgcgcgg cccctcgacg ggcttgccgc
     6061 ccttggccgt gatcttcgcc gtctcggtct tcacgcggcg cacc
//
LOCUS       DASVES010000361        15557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_833744, whole genome shotgun sequence.
ACCESSION   DASVES010000361 DASVES010000000
VERSION     DASVES010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15557
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833744"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF240_14715"
     CDS             complement(<1..260)
                     /locus_tag="VF240_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX7176515.1"
                     /translation="MTGATDMRQYQDLLKVILSRGVEHSDRTGVGTISYFGYQTRFDL
                     RQGFPIVTTKRVPFRWIFEELNWFLSGDTDEATLRARGVDIW"
     gene            complement(438..941)
                     /locus_tag="VF240_14720"
     CDS             complement(438..941)
                     /locus_tag="VF240_14720"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7176516.1"
                     /translation="MPPLSDKSFERARSVYMLMFQAQDPNFESRVRESFARQQLMQTL
                     GAALTLVAPGLVEIELPYQASLTQQHGFLHAGVIASVLDSACGYAAFSLMPADAAVLS
                     VEFKVNLLAPARGERLLARSEVKRAGRTVTVCTADAFAVEGEQSKVVATMLGTMMCLR
                     GRGDLVG"
     gene            complement(925..1329)
                     /locus_tag="VF240_14725"
     CDS             complement(925..1329)
                     /locus_tag="VF240_14725"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX7176517.1"
                     /translation="MQKIKLILKFLFAAFFVLAGLNHFTNADFYLKIMPPYLPLHLLL
                     VYLSGAAEIVLGLLLLIPKFTRVAAWGLIALLVAVFPANIHMAINHEFYPEYNVAAVW
                     LRLPLQLVLIALAYWYTLPVAQRGRGGDAAAV"
     gene            1686..2294
                     /locus_tag="VF240_14730"
     CDS             1686..2294
                     /locus_tag="VF240_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176518.1"
                     /translation="MLPQVLLTLLSNPLGLATVASPFIVNHINSEKAKLAAQTAHRES
                     ELKQANEIAGEVSNAMDNLAYLSKQTMFGIVFRKLSTPEDQAKWKAYQEASMRWESTN
                     STTLAQMEMYFGADNAATLKKIQDDFEILASQVDAAFYERTTSKWFIEDKEGTKHDFR
                     KKYFPVWNRLIDEMTELSKEMIRQIQYEEVGSLRHNTNLIQS"
     gene            complement(2549..2911)
                     /locus_tag="VF240_14735"
     CDS             complement(2549..2911)
                     /locus_tag="VF240_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176519.1"
                     /translation="MSQAAKSLFVFGIYLCGLGLLLLLVPNLLLQVFGAPPTNEVWIR
                     INGMFVLCLSFFYVQAARKELTVFIRWTVWARIAVIFYFAAFVLLVSAPKALLLFGLI
                     DLLSAIWTWLALKKDEAH"
     gene            complement(2943..3404)
                     /locus_tag="VF240_14740"
     CDS             complement(2943..3404)
                     /locus_tag="VF240_14740"
                     /inference="COORDINATES: protein motif:HMM:NF044878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176520.1"
                     /translation="MNREEFEQFKHESFEYLKTQQGRLVNEFGMGGYERWDYDQDTGE
                     FIFSDNGVPKVGADFQVVGSISTISDTWLWSWANPSILDDRKKDMHRVRSFGEEHGLT
                     ELTEERWPADEDAGWAMTNIAARILGARGAYRCPVDNGFLFLIFTDVWRVA"
     gene            complement(3434..4141)
                     /gene="pepE"
                     /locus_tag="VF240_14745"
     CDS             complement(3434..4141)
                     /gene="pepE"
                     /locus_tag="VF240_14745"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HEX7176521.1"
                     /translation="MRLLLLSNTKNPGAPALAHAEGAIKDLLGARRRSVLYVPYATVM
                     WSWDESAVTMRAVFERMGLACESVHTAADPAAAVERAEAVFVGGGNTFQLLRALYDTR
                     LISALRERVRAGMPYIGASAGTNVVCPTIKTTNDMPIIEPPTFDALSLVPFQINPHYT
                     EARLEGHGGETRDDRIAEFLRVNPGVYVVGLREGTMLRVDGSEARLIGARTARVFYGG
                     RASVECGAGDSLEFLFS"
     gene            complement(4262..5794)
                     /locus_tag="VF240_14750"
     CDS             complement(4262..5794)
                     /locus_tag="VF240_14750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7176522.1"
                     /translation="MTTRARRRRERARHSADALCGYNPMMPEEVRVLRLGEMILRRKG
                     RPAGRLVQAIVSVALRLFFRRIETSNASAVPTAGPLIFVLNHPNGLIDPALVFCALPR
                     RISFLAKSTLFRMPVVGSLMRAVDALPLYRRVDEGEDTARNRRTFEACHALLARGRCI
                     AIFPEGVSHDEPRLLPLKTGAARIALGAAAVGAAADGGEEGFGVDTETGAGLDLQIVP
                     VGLYYTSKTTFRSEALLRFGEPFGVPLVALAEDGDPPREAVRRLSARIEEGLRAVTLN
                     VETQGELEVAKRAEQLFSSLYEGIRIETSVAEQFHFLRRFSLGRRMPARSDEGERGRR
                     LHERLLRYDEELRRVGLAPENLSVSSHSAWFVFRHFLLRGAALLLLSPLALIGAALHL
                     PAYAGATVLSLLFPRHGPDVAAATAKILASILLVPLTWLVVSAVVLLLWGWRAALFAL
                     PAAALCGYVAMRTLEELYDMRGWFKAAFLLLRQRHLFLRLLLERRSLHNELKALGDDA
                     GR"
     gene            5905..6150
                     /locus_tag="VF240_14755"
     CDS             5905..6150
                     /locus_tag="VF240_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7176523.1"
                     /translation="MADEDNTNPEWGENESAESASAPSPEVTHTVKAGDTLSKLAREY
                     YGDDKHHRRIFEANRDTLDDPDDIKVGQELKIPPATE"
     gene            6370..9264
                     /locus_tag="VF240_14760"
     CDS             6370..9264
                     /locus_tag="VF240_14760"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176524.1"
                     /translation="MTPERWEQISRVFKSALERRGVERAEYLTAACAGDTELRREVEI
                     LLGSFDTEGAPVSNDGHAPAGEEPQLNVAEGAPPGLEGREVGRYIIREPLGAGGMGVV
                     YRAEDRVLGRAVAIKFIEKGGDHVGNAARRFLREARAASQLNHPNIVVVHEIAETDEH
                     SYLVMELVRGSSLREHIRRRSLSLPEALDIAAQTASALADAHAHSLIHRDIKPENILV
                     DQRGTAKLADFGLARAFEATRPLSQEGAPTLAGVESLTGAGAVVGTLPYMSPEQLRKE
                     PLDERTDVFSFGIVLHEMVAGRHPFAAASSFEIAAGILGRGRVETADLPAGLPEALRV
                     LLVRLLEKDRVRRASSFVEIGRELNQIKRLVEGSASGAEGMMATQRFGAPVEAQNQTG
                     RPTAPEVTSPTGRRTTPVSIVHDSSHHPAGASAQSLWTGAPRTVLVLPFEAVGTQEET
                     SFIGVGLASVIMTALAKVGDISVLSKAAGAGRAIETGLGATELARELGANILLEGEVM
                     RAGKNLRINARLSDVESGRVIWSDQYRGDESELFQIQDAVCESVASALKVSVSREARV
                     EMAQPATNNIDAFELYSKGRAFLERRDQKGNVDLAAGMFYEALRMDQQFALAQAGLGE
                     CFWLKYEATREQLWVERAVAASDRALVLDPRQAQVRITLGIVYHGTGRLERAVEELER
                     ALELQPLSDDAHRWLGQCFMQLDQMERARSHFERAVELRPGYWDNYNWLGNFYYIAGD
                     YTAAAEQFRRVIMFQPDSHLGYNNLGGIHCVLGRYDDAAAMHRRAIEIRPAATTYSNL
                     GTDYFHLGLYEKALAAYRSAVEHEPGKDIYRRNLGDAFLRLGREEEARAEYERACALL
                     AEGLAVKPNNARQLGRLAVCQAKLGRAAEARLSAERATALEPRNTHLMYQSAVVHALT
                     GDEAGAADWLGRALKHGYSRTEVECDPDLDALRESAAYKSLLGQPPAGVS"
     gene            complement(9443..9985)
                     /locus_tag="VF240_14765"
     CDS             complement(9443..9985)
                     /locus_tag="VF240_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176525.1"
                     /translation="MMKRTLPLLLLLALACATGCEPKKDTPNANVNTSTNVGIRQENA
                     NVNSNANNTIAPALHTKEDKTVALIIGEDAAGKLQIFVAPEDIKLLKSKNQKLRFHVF
                     NNTEVDLKEVVITFVTENPMDGNFTVGDIKAGNDKNSSTQKIKTGVADGKYVYGIKAI
                     GNTSPDPVAVMNSPEVEIAT"
     gene            10639..11061
                     /locus_tag="VF240_14770"
     CDS             10639..11061
                     /locus_tag="VF240_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7176526.1"
                     /translation="MSMKLYVGNLSFQTSSEDLQQLFGQAGTVESASVVEDRETGRSR
                     GFGFVEMATAEEGQAAITQFNGKEVNGRALTVNEARPREDRGGRGGGGGGGGRGGFGG
                     GGGGGRGGYGGGGGGGRGGYGGGSGGSGGGGGNREPRW"
     gene            11203..12432
                     /locus_tag="VF240_14775"
     CDS             11203..12432
                     /locus_tag="VF240_14775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7176527.1"
                     /translation="MDFSQLGLQPALVRVCESLGYTEPTPIQKQAIPVILGGADLIGC
                     AETGTGKTAAFLLPLIQRMSEQARPGVRLLVLAPTRELATQIDASYRSLAPKKNALRC
                     VTVIGGTGMASQTAGLRRGASVVVATPGRLLDHMERGAVNFATLDALVLDEADRMLDM
                     GFLPSIRRILAALPKQRQTMLFSATMSEAIEQLARTHMRSPKMVEVNQRNKPPGLVAQ
                     TAYPVATESKTALLLDLLEREREEFERVLVFTRTRRGAERLSHILEAREHKVNRIHAD
                     RTQPQREAALRGFKDGKTRVLVATDIAARGIDVDSISHVINYDVPETPEDYVHRIGRT
                     GRAGNPGRAITLLTPVEELSMRAIERLTGQAVERVLLPGFGGMQPSGTGGPGARRTAS
                     AGRGQLGRRSFRPRRGR"
     gene            12565..12927
                     /locus_tag="VF240_14780"
     CDS             12565..12927
                     /locus_tag="VF240_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176528.1"
                     /translation="MGSEMFAVGQRVDKLCAVCGVERGHVVASVTKRGQISRVSCPTC
                     GTRSSFKSGSRTSGARDGAQSGAPYDPTRTYRTGQSMMHPAFGFGEVTAVIEPQKIDV
                     LFTDRLRRLIHSRTPAAT"
     gene            13211..13864
                     /locus_tag="VF240_14785"
     CDS             13211..13864
                     /locus_tag="VF240_14785"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176529.1"
                     /translation="MTLTRNRSLNVVGSLLTLLLLSVASFAQEAAPPQQQALTKESFP
                     QVRIKNFGQMDARFYRGAQPKEDQYETLKALGITTVIDLQAEPKEYERRIVESLGMRY
                     VNIPMVEKKYPKPEDVAAFLKLADDPATGKFYLHCAGGRHRTGVMGAVYRFNKYGWDY
                     EHVYREMKDFDFYTAWGHGAFKDFVQDYWQQVQTAKLSAPAVQPAPAEAVFVGGNKK"
     gene            14123..14224
                     /locus_tag="VF240_14790"
     CDS             14123..14224
                     /locus_tag="VF240_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7176530.1"
                     /translation="MKKIKQAGFTCIELAVAASILGGMLLALLPAVQ"
     gene            complement(14304..>15557)
                     /locus_tag="VF240_14795"
     CDS             complement(14304..>15557)
                     /locus_tag="VF240_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176531.1"
                     /translation="HASEGRARILRNFYELGRRSLDYPPADSRDPVAYVIPAGQGRDE
                     NLSKMIGALVEQGVEVHRMDSELHLSHTSRDARDAHETPGGSYLVLLAQPYRTNVQAL
                     FERQVYPDRRTASGEAERPYDVAGWTLPMQMGLEVLPVVGIREPARERRLTPVLSEDD
                     VRRDLGLPPRLGDASPVRSPLERPVRLALYKSWTASMDEGWTRYVFDTFNVPYTSLRD
                     RDVRAGNLRQKFDVIVLPSMRTREIVEGRPPVRAPAEFAGGITEAGVEHLRRFVEEGG
                     TLVCFDASTELAIKRFRLPVKNVLEGLKSSDFYGPGSILRIEVDTTHPIARGMAREAD
                     AYFIQSQAFEVEEREGVRVVARYAGKDAVLRSGWLLGPQHLAGKAALVEVALGRGRVR
                     LFGFRPQHRAQTWGTFPFIFNAISR"
BASE COUNT         3034 a         4960 c         4925 g         2638 t
ORIGIN      
        1 ttccagatgt ccacgccgcg cgcgcgcaaa gtggcctcgt cggtgtcgcc ggagagaaac
       61 cagttcaact cttcaaaaat ccatcgaaaa ggcacccgct tggtcgtcac gatggggaac
      121 ccctgacgca gatcgaatct cgtttgatac ccgaaatatg aaatcgttcc gacgcccgtg
      181 cggtcggaat gctccacgcc acgactcaga atgaccttca gcaaatcctg atactgtctc
      241 atgtctgttg ccccggtcac gactgacctg acacgtgtca tctatccccg aatcaaccca
      301 cgccgaagcg aatagcacac agcatacgcc ggatgatacg agtagcaaag aaaagtcatt
      361 gaggggtctg gttgaatata aaagacctgc ggagttgcgc gtccgctgtg cggcgtcgtc
      421 aggctgatga gagtgattta accgacgagg tcgccacgcc ctcggagaca catcatggtt
      481 ccgagcatcg tggccacgac cttgctctgc tctccctcga ccgcaaacgc gtcggccgta
      541 cacacggtca ctgtccgccc cgcgcgcttc acctccgagc gcgccagcag acgttcgcca
      601 cgggccggcg cgagcaggtt gaccttgaac tcgacgctca agaccgccgc gtccgcgggc
      661 atcaggctga acgcggcgta cccgcacgca ctgtcgagca ccgaggcgat gacgcccgcg
      721 tgaagaaagc catgctgttg cgtcaggctg gcttggtaag gcagttcgat ctccaccaag
      781 ccgggcgcga caagcgtcaa cgcggccccg agcgtttgca tcaactgctg gcgggcgaaa
      841 ctttcgcgaa ctcggctctc gaagtttggg tcctgagctt gaaacatcaa catataaaca
      901 ctgcgcgccc gctcgaacga cttgtcagac agcggcggca tccccacctc tccctctttg
      961 agcgacaggc aacgtgtacc agtaagccaa tgctatgagc acaagttgaa gcggcaatct
     1021 taaccacact gccgcgacat tatactccgg gtaaaactcg tgattgatcg ccatgtgaat
     1081 gttcgcagga aagaccgcga ccaacagcgc gatcagaccc caggccgcca cgcgcgtgaa
     1141 cttcggaata agaagtaaga ggcccaaaac tatttcagcc gccccgctca gataaacgag
     1201 gagcagatgc aagggcaggt aaggcggcat aatcttcagg tagaagtcag cattggtaaa
     1261 gtgattgagg ccagccagca cgaagaacgc cgcaaataaa aatttcaaga taagcttgat
     1321 tttctgcatc cgccacagct tacgataact cgccctttgc ggcctatcgc cggaattaac
     1381 ccgcgccgag cgagcagcat tctagcaccg gctcaataaa gagcgatgaa aagaatgcta
     1441 tcgaggcgtc gggttcaatg agttgttcgg cgcggcatcc atcctgccgt tgcgacagcc
     1501 aatctgctgc tcggcatgaa gctctcaact gactaacgct tcagcgttga catatctctg
     1561 aaactgaagc tatcttgctt cagcttgtga tagcttatta ctcgtaatct tcatgcgggc
     1621 gagttagaag gcaacgacaa ccctccctat catcaacatc atcctgaggc tattggaggt
     1681 gctaaatgct tccccaagta ttgttgactc tcctaagtaa tcccttgggt ctggctacgg
     1741 tagcatctcc atttattgtg aatcacataa actcggagaa agctaagctt gccgcccaga
     1801 cggctcatcg ggagtcggaa cttaaacagg cgaatgaaat agccggtgaa gtcagtaacg
     1861 cgatggataa tctggcttat ctcagtaaac agacaatgtt tggtatcgtt ttccgaaaac
     1921 taagcactcc tgaagatcag gcgaagtgga aggcgtacca ggaagcatca atgagatggg
     1981 aaagtactaa ttcaacaacc cttgctcaaa tggagatgta tttcggtgcg gataacgccg
     2041 caacccttaa aaagattcaa gatgattttg agattttagc aagccaagtt gacgccgctt
     2101 tttacgaaag gacaaccagc aagtggttta ttgaggataa ggaaggtact aagcatgatt
     2161 ttaggaaaaa gtactttcct gtttggaata ggttaataga cgaaatgacc gagctttcca
     2221 aggaaatgat tagacaaatt cagtatgaag aagttggttc ccttcgacat aacaccaacc
     2281 ttatccagtc ttgatccagc gttattctcc ggtgactgtc gcctcgccag acttgcgagc
     2341 gtggcagtgc cgtagcattc ttctcactta gcacctcatt ggcctgagaa aaaaacagtg
     2401 gccttaccgc cgaacgctgg gattgagccg ccgcgcacac aacgacacca gccttcaatc
     2461 tgacgggtga aagccgcgct ctttcgcggt tggcttgaat gatttattcg gcggcgtgtt
     2521 gttatccacg cgcctcagaa tatgttcttc aatgagcttc atcttttttc aaagcaagcc
     2581 atgtccaaat cgccgacaac aaatcaatca ggccaaacaa caacaacgct ttcggagccg
     2641 agaccaacag cacaaaagcg gcgaagtaaa aaattaccgc gatgcgcgcc cacaccgtcc
     2701 agcggataaa aactgtcaac tcctttcgcg ccgcctgaac gtaaaagaag ctcagacaaa
     2761 gcacaaacat tccattgatt cttatccaca cctcgtttgt cggcggcgcc ccgaaaacct
     2821 gtaaaagcag atttgggacg agcagcaaaa gcaaacctaa tccgcagagg taaattccga
     2881 agacaaacaa acttttagcc gcttgactca taccttaaaa attaagtttt tactctgccg
     2941 cgtcaggcga cgcgccacac gtccgtgaaa attaggaaga ggaagccgtt atcgacgggg
     3001 cagcggtagg cgccgcgggc gccgagtatg cgggcggcga tgttcgtcat cgcccagccg
     3061 gcgtcctcgt cggcgggcca acgctcctcg gtcagctcgg tcaggccgtg ctcctcgccg
     3121 aagctgcgga cgcggtgcat gtcctttttt ctgtcgtcga ggatgctcgg gttggcccac
     3181 gaccagagcc acgtgtcgga gatcgtggag atggagccaa ccacctgaaa gtccgcgccc
     3241 accttgggga cgccgttgtc ggagaagatg aactcgccgg tgtcctgatc gtagtcccag
     3301 cgctcgtacc ctcccatgcc gaactcgttg acgagccgcc cctgctgcgt cttgaggtac
     3361 tcgaaagact cgtgcttgaa ctgttcgaat tcttcccggt tcattcccgc cccgaatcgt
     3421 cttgcgactg actttaactg aaaaggaact ccagcgaatc gcccgcgccg cactcgacgc
     3481 tggcgcggcc gccatagaag acgcgcgccg tccgcgcgcc gatgagtctc gcctcagagc
     3541 catcgacccg gagcatcgtc ccctcgcgca gcccgacgac gtagacgccg gggttgacgc
     3601 gcagaaactc cgcgatgcgg tcgtcgcgcg tctcgccgcc gtgtccttcg agtcgcgcct
     3661 ctgtgtagtg agggttgatc tggaagggga cgagagagag cgcgtcgaag gtgggcggct
     3721 cgatgatggg catgtcgttc gtggtcttga tggtcgggca gacgacgttc gtgcccgcgc
     3781 tcgcgccgat gtagggcatc ccggcgcgga cgcgctcgcg cagggcgctg atgagacgcg
     3841 tgtcgtagag cgcgcgcagc agttggaagg tgttgccgcc gccgacgaag acggcctcgg
     3901 cgcgttcgac cgccgccgcc gggtcggccg ccgtgtgtac cgactcacag gcgagtccca
     3961 tgcgctcgaa cacggcgcgc atcgtgacgg cgctttcgtc ccaactccac atgacggtcg
     4021 cgtagggcac gtagaggacg gagcggcggc gcgcgccgag gaggtctttg atggcccctt
     4081 cggcgtgcgc cagcgcgggc gcgccggggt tcttcgtatt gctgagaagg agaagtctca
     4141 tcgtggtgtt agatttcagt cgagtgtcgg gtaactaaga ctcaccgcag actcaggtaa
     4201 aaattcacac tgaagtctag ccgagccagc ggctcgaagg aaacgacgcg tgcagcctcg
     4261 tttagcgacc cgcgtcgtcg ccgagcgcct tcaactcatt atgcagcgag cggcgttcga
     4321 gcagaagccg cagaaagaga tgtcgctggc gaagcaggag gaatgcggcc ttgaaccagc
     4381 cgcgcatgtc gtagagttct tcgagcgtcc gcatggcgac gtagccgcac agggcggcgg
     4441 ccggcagggc gaagagggcc gcgcgccagc cccagagcag gaggacgacg gccgagacga
     4501 cgagccacgt cagcggcacg agcaggatgg aagcgagaat cttggcggtg gcggcggcca
     4561 cgtcggggcc gtggcgtggg aagaggaggg acaacaccgt cgcgccggcg taggccggaa
     4621 gatgcagggc cgcgccaatc agcgccagcg gcgagagcaa caggagcgcc gccccgcgca
     4681 gcaggaagtg tcggaagacg aaccacgccg agtgagacga gacggagaga ttttccggcg
     4741 caagccccac gcggcgcagc tcctcgtcgt agcggagcag gcgttcatgc aagcgccggc
     4801 cgcgctcgcc ttcgtcgctg cgcgcgggca tgcgccgccc cagtgagaag cgccggagaa
     4861 agtggaactg ctcggcgacc gaggtctcga tgcggatgcc ctcgtagagc gaagagaaga
     4921 gttgttcggc gcgcttcgcc acttccagct caccttgcgt ctccacgttc agcgtcaccg
     4981 cgcgcaagcc ctcctcgatg cgcgcggaga gccgtcttac ggcttcgcgc ggcgggtcgc
     5041 cgtcttccgc gagcgcgacg aggggcacgc cgaaaggctc gccgaagcgc aacagcgcct
     5101 cgctgcgaaa agtggtcttc gacgtgtagt aaagtccgac ggggacgatc tgcaaatcca
     5161 gacccgcgcc cgtctccgta tcgacgccga atccttcctc gccgccgtcg gcggcggcgc
     5221 cgacggccgc cgcgccgagc gcgatgcgcg ccgcgcccgt cttgagcgga agcaggcgcg
     5281 gctcgtcgtg cgagacgcct tcggggaaga tggcgatgca acgcccacga gctaacagcg
     5341 cgtgacacgc ctcgaacgtc cgacggttgc gcgccgtgtc ttcgccttcg tccacgcgcc
     5401 ggtagagcgg caacgcatcg acggcgcgca tcagtgagcc gaccacgggc atgcgaaaaa
     5461 gcgttgactt ggcgaggaaa gagatgcgcc gtgggagcgc gcagaagacg agcgccgggt
     5521 cgatgaggcc gttcgggtgg ttgaggacga aaatgagcgg ccccgccgtg ggcaccgccg
     5581 atgcgttact cgtttcgatg cgccggaaga acagacggag cgcgacactg acgatggcct
     5641 gcaccagacg ccccgccggc cgacccttcc gccgcagaat catctcgccc agccgcagca
     5701 ctcgcacctc ttccggcatc atcggattat agccgcaaag cgcgtcggcc gagtgccgtg
     5761 cgcgctcccg ccgccgccgc gcgcgcgtcg tcatttgacc tgagggcgga actgttatag
     5821 aagcggagta gtgcgagtac gaccgaaagg gacaggtccg aggacgaccg tcatcaaatt
     5881 ccgctacacc gggaggagaa aacaatggca gacgaagata acaccaaccc cgaatggggc
     5941 gagaacgagt cggccgagag cgcgagcgcg ccttcgcccg aagttacaca caccgtgaag
     6001 gcgggcgaca cgctctcgaa gctcgcccgc gagtattacg gcgacgacaa gcaccaccgg
     6061 agaatcttcg aggccaaccg cgacacgctc gacgacccgg acgacatcaa ggttggacag
     6121 gaactgaaaa tcccgcccgc caccgagtaa gcttcaacgg ccccgaaggg agggcgcccc
     6181 gcttgcggga tcgccctccc gccacacttg ccgcccgccc cgttcggcgc gcgcgaggcc
     6241 cgccgagacc cgcaagattc ttcctcttga catttccctg acgtgtacta gattgaccgc
     6301 gtgaacgctc cgccgccgcc catgcgaggc gaaggcggcg cccgccgaat tttttacgag
     6361 gcagcccgaa tgacgcccga gcgttgggag cagatcagcc gcgtgttcaa gtccgcactg
     6421 gagcggcggg gcgtggagcg cgcggagtac ctgacggcgg cctgcgccgg cgacacggag
     6481 ctgcggcgcg aggtcgaaat ccttctcggc tccttcgaca cggaaggcgc gcccgtttct
     6541 aatgacggac acgcacccgc cggagaggaa cctcagctca atgtcgcaga gggcgcgccg
     6601 cccggtctgg aggggcggga ggtcggccgc tacatcatcc gtgagccgct cggagcgggc
     6661 gggatgggcg tcgtctacag ggccgaggac agggtgctgg gccgcgccgt cgccatcaag
     6721 ttcatcgaga agggcggcga ccacgtgggc aacgccgccc gtcgtttcct gcgcgaggcc
     6781 cgcgccgcct cgcagctcaa ccaccccaac atcgtcgtcg tccacgagat cgccgagacg
     6841 gacgagcact cctacctcgt catggaactg gtgcgcggct cgtcgctgcg cgagcacatc
     6901 cgccggcgct cgctctcgct gcccgaggcg ctcgacatcg ccgcgcagac cgcttcggct
     6961 ctcgccgacg ctcacgccca ctccctcatc caccgcgaca tcaagcccga gaacatcctc
     7021 gtcgatcaac gcgggacggc gaagctcgcc gacttcgggc tggcccgcgc cttcgaggcg
     7081 acgcgcccgc tctcccagga gggcgcgccc accctcgccg gcgtcgagtc gcttaccggc
     7141 gcgggcgccg tcgtcggcac ccttccctac atgtcgcccg agcagcttcg caaggagccg
     7201 ctcgacgagc gcaccgacgt gttcagcttc ggcatcgtcc ttcacgagat ggtcgcgggc
     7261 cgccacccct tcgccgccgc cagctccttc gagatcgccg cgggcatcct cggcaggggc
     7321 cgggtcgaga ccgccgacct tcccgccggt ttgcctgaag cactgcgcgt gctgctcgtg
     7381 cgcctgctcg aaaaagaccg cgtgaggcgc gcgtcgtcct tcgtcgagat cgggcgcgag
     7441 ttgaatcaga tcaagagact cgtggaaggc tcggcctccg gcgccgaagg aatgatggcg
     7501 acgcaacgct tcggcgcccc ggtcgaggcg caaaaccaga cgggccgccc caccgcgccg
     7561 gaagtgacat cccccacggg gcgtcggaca acacccgtct ccatcgtcca cgattcgtcg
     7621 caccacccgg ccggcgcctc cgcgcagtcg ttatggacgg gcgcgccgcg caccgtcctc
     7681 gtgctcccct tcgaggccgt cgggacgcag gaggagacga gcttcatcgg cgtcggtctg
     7741 gcctccgtca tcatgacggc gctggcgaag gtcggcgaca tctcggtgct gtcgaaggcg
     7801 gccggcgctg ggcgcgccat cgagacgggg ctgggggcga cggagctggc gcgggagctg
     7861 ggcgcgaaca tcctgctcga aggcgaggtg atgcgcgccg gaaagaattt gagaatcaac
     7921 gcgcgtctga gcgacgtgga gtcgggccgc gtcatctgga gcgaccagta ccgcggcgac
     7981 gagtcggagc tgttccagat tcaggacgcc gtctgcgagt cggtcgcctc ggccctcaag
     8041 gtcagcgtct cgcgcgaggc gcgcgtcgag atggcgcaac cggcgacgaa caacatcgac
     8101 gccttcgaac tctactcgaa gggccgcgcc tttctcgaac gccgcgacca gaagggcaac
     8161 gtcgatctgg ccgccggcat gttctacgag gcgctacgca tggatcagca gttcgcgctg
     8221 gcgcaggcgg gtctcggcga gtgcttctgg ctgaagtatg aggcgacgcg cgaacagctc
     8281 tgggtggagc gggccgtggc ggccagcgac cgcgcgctcg tcctcgaccc aaggcaagcg
     8341 caggttcgca tcacgctcgg catcgtctac cacggcacgg gccgcctcga gcgcgccgtc
     8401 gaggagcttg agcgcgccct cgaactccag cccttaagcg acgacgcgca ccgctggctc
     8461 gggcagtgct tcatgcagct cgaccagatg gagcgcgccc gctcccactt cgagcgggcc
     8521 gtcgagcttc gccccggcta ctgggacaac tacaactggc tcggcaactt ctactacatc
     8581 gccggcgact acacggccgc cgccgagcag ttccgccgcg tcatcatgtt ccagcccgac
     8641 agtcacctcg gctacaacaa cctcggcggc atccactgcg tcctcggccg ctacgacgac
     8701 gccgccgcca tgcaccgccg cgccatcgag attcgcccgg ccgccaccac ctactcgaac
     8761 ctcggcacag actactttca cctcggtctc tacgagaagg cgctcgccgc ctaccgtagc
     8821 gccgtcgagc atgaacccgg caaagacatc taccggcgca acctcggcga cgccttcctg
     8881 cggctcggca gggaagagga ggcgcgcgcc gaatacgagc gggcgtgcgc gcttctggcc
     8941 gaaggactcg cggtcaagcc caataacgcg cggcagctcg ggcgtctcgc cgtctgtcag
     9001 gccaagctgg ggcgcgcggc cgaggcccgc ttaagcgcgg agcgcgcgac cgcacttgag
     9061 ccgcgcaaca cacacctcat gtaccagtcg gccgtcgtcc acgcgctcac gggcgacgag
     9121 gcgggggccg ccgattggct cggccgcgcc ctgaagcacg gctacagccg gacggaggtc
     9181 gagtgtgacc ccgacctcga cgcgctgcgc gagagcgccg cctacaagtc tctcctcggc
     9241 cagccgccgg ccggcgtctc ctgacgcgcc gccgcgcgcg acggcccgcc cgcgagtcct
     9301 gcaagcagtt ttcacaccac ctgtctgatg taattcgcca cgcatttaga ggcgggccgc
     9361 gcttgccggc ccgtgcataa agtgtcggga cggcgagcgc ggcccgcctc tgaaattcac
     9421 tggtaagcta aaacgaaacg ctctaggtgg cgatctccac ttcgggggaa ttcatcacgg
     9481 ccaccgggtc gggggaggtg ttaccgatgg ccttgatgcc gtagacatac ttaccatcgg
     9541 ccaccccggt cttgatcttc tgcgtcgagc tgtttttgtc gttgccggcc ttgatgtcgc
     9601 cgaccgtgaa gttgccgtcc atcggattct cggtcacaaa ggtgatgacg acttccttaa
     9661 ggtctacctc cgtgttgttg aagacgtgga agcggagctt ctgattcttc gacttcaaga
     9721 gcttgatgtc ctcgggcgca acgaagattt gcagcttgcc cgcagcgtcc tcgccgatga
     9781 tgagcgcgac ggtcttgtct tctttggtgt ggagggcggg ggcgatggtg ttgttggcgt
     9841 tggagttgac gttcgcgttt tcctgcctga tgccgacgtt ggtgctggtg ttgacgttgg
     9901 cgttgggagt gtccttcttc ggctcgcagc ccgtggcgca agccagggcg agcagcaaaa
     9961 gcagcgggag tgttcgcttc atcattgagg ggtttctcct gtctgagagg gcttcgggaa
    10021 agtggccggt cgcgcgccgt gtcgcgccgc acggccgggg ccgcagagag ataggactgt
    10081 cgagcgccga gtcgtggaag ggcgcggccg ggaaggcgcg gccgcactgg ccggatcggt
    10141 caaacgtccg agcgtcgtca cacccttgag tccgagaaag cgtgcggatt gtacggcgaa
    10201 ttctcacaaa ctgtccacag aaatcttgga cgagcgcggc tccgtttcat cgccgcgcgg
    10261 ccacgcgggc cgctcatccg tgggcggcct tctcgacgtg taatcgacgg ctgaaattaa
    10321 tgtctcgcgg ggcgaattga cagttgaact gttcacgacg acggaggctg tcgcggccca
    10381 cggcttgcaa ccggggccgc taatagtgca tcatattgca cggttagtcc ttctctcttg
    10441 catcaacggt gatgcgccac gagcttgttt tgtctgctgc ggcgttggga tggttcgaga
    10501 gttggttgat ttcaatttta gaagccgctc ctcaagagca tccgaacgcg agccttcgct
    10561 gcccaccgtt acatcgaagc cacttaaatt cgcagcttct ctcagacact cctgcgctaa
    10621 aggagaaggt acacgtatat gtcaatgaaa ctctatgtcg ggaacctgtc gttccagact
    10681 tccagcgaag acttgcagca gttgttcggg caggccggga ctgtggaatc ggcctcggtg
    10741 gtggaagacc gtgagacggg ccgctcgcgc ggcttcggct tcgtcgagat ggcgacggcc
    10801 gaggaggggc aggcggcgat cacgcagttt aacggcaagg aagtcaacgg gcgggcgctg
    10861 acggtcaacg aggcgcggcc gcgagaggat cgtggaggac gcgggggcgg cggcggcggc
    10921 ggcggtcgtg gcggcttcgg cggcggcggc ggcggcggtc gtgggggcta cggcggaggc
    10981 ggcggcggtg gacgcggcgg ctacggcggt ggttccggcg gctcgggcgg cggcggcggc
    11041 aaccgcgagc ctcgctggta agcacacggt cggcccgagg gccgactcaa ttcaagcgaa
    11101 gcgcccccgg cgatgtcgaa tcccgccggg ggcgctcaca aactttcagg taagagaagg
    11161 cgctcgcgcg ttcaagcgtt acagtagcgc cgaaggaaat tcatggactt ttctcaactg
    11221 ggactccagc ccgcgctcgt gcgcgtctgc gagtctctcg gatacaccga accaacaccg
    11281 attcaaaaac aggcgattcc tgtgattctc ggcggcgccg acttgattgg ctgcgccgag
    11341 accggcaccg gcaagacggc cgcgttcctt ctcccgctca tccagcggat gtcggagcag
    11401 gctcgccccg gcgtccggct gctcgtcctc gcgccgacgc gcgaactggc gacccagatc
    11461 gacgccagct accgctcgct cgcgcccaag aagaatgcgc tgcgttgcgt gaccgtcatc
    11521 ggcggcacgg gcatggcgag tcagacggcc ggcctccggc gcggcgcgag cgtcgtcgtc
    11581 gcaacgcccg gccgactgct cgaccacatg gagcgcggcg cagttaactt cgcgaccctc
    11641 gacgctctcg tgctcgacga ggccgaccgc atgctcgaca tgggcttcct gccctcgatc
    11701 cggcgcatcc tcgcggcgct gccgaaacag cgtcagacga tgctcttctc ggccacgatg
    11761 tcggaggcga ttgaacaact cgcgcgcacg cacatgcgct cgccgaagat ggtcgaagtc
    11821 aatcagcgca acaagccccc ggggctggtc gcacagaccg cctacccggt ggccacggag
    11881 tcgaagacgg ctctgctgct cgatttgctg gagcgcgagc gcgaagagtt cgagcgcgtg
    11941 ctcgtcttca cgcgcacgcg gcgcggggcc gaacggctct cgcacatcct cgaagcgcgc
    12001 gagcacaagg tgaaccgcat tcacgccgac cgcacgcagc cgcagcgcga ggccgccctg
    12061 cgcggcttca aggacggcaa gacgcgcgtc ctcgtcgcca ccgacattgc ggcgcgcggc
    12121 atcgacgttg actccatctc gcacgtcatc aactacgacg ttcccgagac gccggaagac
    12181 tacgtacacc gcatcggccg cacgggccgg gccggcaacc ccggtcgcgc catcacgctg
    12241 ctcaccccgg tggaagaact gtcgatgcgc gccatcgaac ggctcacggg gcaggcggtc
    12301 gagcgcgtcc tgctgcccgg cttcggcggc atgcagccat cgggcacagg aggcccgggc
    12361 gcgcgtcgca cggcctcggc cggacgcggc cagctcggtc gccgctcctt ccgcccgcgg
    12421 cgcggcaggt aacgcgcggc gcgtgaactt ctcacgcgcc ccctcgctcg tcaaagccgc
    12481 ccgctgcggc cgtcatcgtt tgtgaatggt gcttcggcgt ccgctcggcg agtgggcgcc
    12541 gaaaacagaa aggaagagtg agtgatgggg agtgaaatgt tcgcggtcgg ccagcgggtg
    12601 gacaagctgt gcgcggtgtg cggggtcgag cgcggtcacg tcgtggcgtc cgtcaccaag
    12661 cgtgggcaga tttcgcgcgt gagttgtccg acctgcggca cgcggagttc gttcaagagc
    12721 ggctcccgaa cgtcgggcgc gcgcgacggg gcacagtccg gcgcacccta cgacccgacg
    12781 cgcacctacc gcacgggcca gagcatgatg caccccgcct tcggcttcgg cgaagtgacc
    12841 gcggtcatcg agccgcagaa gatcgacgtg ctcttcaccg accgcctgcg ccgtctgatt
    12901 cacagccgca cgcctgccgc aacctgaagt gaagcgtcgt acactcgcga ccccggccgg
    12961 cgtcctcaat cgacggtcaa tgtgaatagg gcttaagtaa taaagttcag gtaagataag
    13021 cgggcgacgc gggcgcggac gcggcggcta gttgatacaa cctggagggc actcccgcat
    13081 ctaaattctc gattgacgat gaggtccctt cagtggcacg atagtcattc gacggacttc
    13141 gccccacccc cacaagcgaa cgacacccct tgaagctgga agctggttct cggactctgg
    13201 aggaagattg atgactctga cccgtaatcg ttccctcaat gtcgtcggtt ccctgttaac
    13261 gcttctacta ctctcggtcg cctccttcgc gcaggaagcc gcgccgccgc aacagcaggc
    13321 gctgacgaaa gagtcgttcc cccaggtcag gatcaagaac ttcggccaga tggacgcgcg
    13381 gttctaccgc ggcgcgcagc cgaaggaaga ccagtacgag acgctcaagg cgctcggcat
    13441 cacgaccgtc atcgaccttc aggcggagcc gaaggagtat gagcggcgga tcgtcgaatc
    13501 gctcgggatg cgctatgtga acatcccgat ggtcgagaag aagtacccga agcccgagga
    13561 cgtggcggcc ttcctgaaac tcgccgacga ccccgcgacc ggcaagttct acttgcactg
    13621 tgccggcggc cggcaccgga cgggtgtgat gggcgccgtc taccgcttca acaaatatgg
    13681 ctgggactac gaacacgtct accgcgagat gaaggacttc gacttctaca ccgcgtgggg
    13741 ccacggcgcc ttcaaggact tcgtccagga ctactggcag caagttcaga ccgcgaagtt
    13801 gagcgcgccg gccgtccagc ccgcgcccgc cgaggcggtg ttcgtcggcg gcaataagaa
    13861 gtaaaagtta agcactcctg agaacgaaga ggggcgcttt cctgataggg gaagcgcccc
    13921 tttcaattca ccaaccaccc tgcccgacga ttacgactcc tgtttggacg actcgcacgc
    13981 ctgagaatat tgttgggcgg agccgcgccc tacgaatttt tgtgtgatcc ctttcgcctg
    14041 acttctccgc gttagtaaat gacggggcgc agatggttct tacgccccgc actaaacagg
    14101 aggagaggaa aaggagcaca ccatgaagaa gattaaacag gccggtttca catgtatcga
    14161 actcgccgtc gccgcgtcga tcctcggggg aatgctgctg gccctactgc cggcggtgca
    14221 gtaatagtcg tcaccaacaa caacaagagg gcggccgagt caccgggaag tgactcggcc
    14281 gccctctttc atctcaagcg tttctagcgc gagatggcgt tgaagatgaa ggggaaggtg
    14341 ccccacgtct gagcgcggtg ttgcgggcgg aagccgaaga ggcggacgcg gccccggccc
    14401 aacgcgacct cgacgagcgc cgccttgccc gcgaggtgct gcggcccgag gagccacccc
    14461 gagcgcagca ccgcgtcctt ccccgcgtag cgcgcgacga cgcgcacgcc ctcccgctcc
    14521 tccacctcga acgcctgact ctggataaaa tatgcgtccg cctcgcgcgc catgccgcgt
    14581 gcgatggggt gtgtggtgtc aacctcgatt cgcaggatcg agccggggcc gtagaagtcc
    14641 gacgacttca gaccttcgag gacgtttttg acgggcagac gaaaacgctt gatggcgagt
    14701 tcggtcgagg cgtcgaaaca gacgagcgtg ccgccctcct cgacgaaacg gcgcaggtgc
    14761 tcgacgccgg cctccgtgat gccgcccgcg aactcggccg gcgcacgcac gggcgggcgc
    14821 ccctcgacga tctcgcgagt ccgcatcgaa ggcaaaacga tcacgtcgaa cttctggcgc
    14881 aaatttccgg cgcgcacatc ccggtcgcgc agggaagtgt aaggcacgtt gaaggtgtcg
    14941 aagacgtagc gcgtccagcc ctcgtccatc gaggccgtcc aacttttgta gagcgcgagg
    15001 cggacggggc gctccagagg gctgcgcacg ggcgacgcgt cgccgaggcg tggcgggagt
    15061 ccgaggtcac gccgcacgtc gtcttcgctg agaacgggcg tcagtcgccg ttcgcgcgcc
    15121 ggctccctga tgccgacgac cggcagcacc tccagaccca tctgcatcgg cagcgtccac
    15181 ccggccacgt cgtaagggcg ctcggcctcg cccgaagcgg tgcgccggtc ggggtagacc
    15241 tgccgctcaa acagcgcctg cacgttcgtg cggtagggct gcgcgaggag gacgaggtag
    15301 ctgccgccgg gcgtctcgtg cgcgtcgcgc gcgtcgcgcg acgtgtgcga cagatgcagc
    15361 tccgagtcca tgcgatgcac ttcgacgccc tgctcgacga gcgcgccgat catcttcgag
    15421 aggttttcgt cgcggccctg accggcgggg ataacgtagg cgacggggtc gcgtgagtcg
    15481 gcgggcggat agtcgagcga gcgccggccg agttcgtaga agtttcggag gatgcgcgcg
    15541 cgtccctcgg aggcgtg
//
LOCUS       DASVES010000362         9713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_640546, whole genome shotgun sequence.
ACCESSION   DASVES010000362 DASVES010000000
VERSION     DASVES010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640546"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="VF240_14800"
     CDS             complement(<1..1808)
                     /locus_tag="VF240_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176532.1"
                     /translation="MALAIGTVIYLVVLKASGLDLVALKGGGVGPGVATLQFIVATAA
                     MALIVMTAGPLKNFLQERIDRFFYGERYDLRHGLLDFGRTLSATTTLEPLLDALVSRL
                     QQVLGVERIAIFIEDARAPSGYRVARAAGLASGLIVPPDFREMIRTRSAQEGIVRADD
                     IDLAPEAVGFVRRALHYYVPCVVRGRMVAVIGLGRSVGGALLSSEDLEILRTVSGYVA
                     VAIENSLLYQEQHERAEELVLLKEFNESIVESINVGLVAVDSEGRVTACNTSLEELLG
                     VRRDDAFGARVEDLFAEDFAETLGQVLGVSRWRLGEVRHIYKLHTAARQGRSLILNIS
                     IAPLKRPDSPDHTGALVVLEDVTGRVRLEEQLQQREKLSSIGLLAAGVAHEINTPLTG
                     VSSYTQMLLGTVADTDPKHALLQKIRRQTDRATGIVNNLLNFSRTGGVTDFAEVDLNR
                     VLEDTLQLLETQLRHSHVEVRREYGPRVPRVFGNPGQLQQVFTNLLLNARDAIPDGGT
                     VTLRTRADEEGDVVTVEVEDDGIGIAAENVARIYDPFFTTKGVGRGTGLGLAVSYGIV
                     QEHAGHISVESSPGRGTRFRITLPTAATRARLQAAS"
     gene            complement(1750..3510)
                     /locus_tag="VF240_14805"
     CDS             complement(1750..3510)
                     /locus_tag="VF240_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176533.1"
                     /translation="MKSSVLTTKAWVLLIAAAVLVAAGTLNFAQRLTHTPPLTDGVEW
                     VQTRDGRIVAASVAPDSAAGRKGVFGIAPDDRLIAISTSDGREGGEAVERVSDVQIFI
                     DESSTTGQLRYLIERPSYPEDTRFYYVYLENLTPLATWTTRDLYVNAVGIVYLLVGLF
                     VFFKQGERAPFVLHFTALCLAAFVFHFYKPFGNFKDVDLAVDFLDHTALQLFAPLFLH
                     FCAIYPVRHRLSDKRRWLAALLYVPALVLIALTAFFDFTVYLRAVLPASIYNALLAAY
                     TGLPDGFAGWLSQAEFVCLMVALAAGSGVLVYRFLRSESAVVRQQLKWVVWGSAVAVT
                     PFTLVYAVDYLSGVGLSSVLSESAARRLTDAAILPLVLIPLAFGNSVVRYRLMDVDMV
                     VRRTAVYALTTLAVALAIGTVIYLVVLKASGLDLVALKGGGVGPGVATLQFIVATAAM
                     ALIVMTAGPLKNFLQERIDRFFYGERYDLRHGLLDFGRTLSATTTLEPLLDALVSRLQ
                     QVLGVERIAIFIEDARAPSGYRVARAAGLASGLIVPPDFREMIRTRSAQEGIVRADDA
                     RRGAGHRHGHLSRGAQGFGP"
     gene            complement(3903..5684)
                     /locus_tag="VF240_14810"
     CDS             complement(3903..5684)
                     /locus_tag="VF240_14810"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7176534.1"
                     /translation="MRKSYMRTGLVLLAIFVACGENAPSAFAAGGGGRRPVGTVTGSV
                     KDSQGNPLAGAVISLLREGAEGIVKEVRSAADGTYKATVAPGTYVLRAVASGFSPTTF
                     PAVTVKASAELVYRFNLEPTGQGLTTPERRADRDDPKYKIRASFRRRHIFNANESAGA
                     ATGETAEGTTDTATATGETMAEETADIDTRRFWASRPHGVVQTFYAASPGDDHGGFAS
                     INFAALTPVSPNVDLVFVGQAGAFDRFEATARVRAGAHHRLSATVGGARLPLGRKGAL
                     LLGDDMLEQLSVRATDEWVVREGVVVVLGLDYSRFLGAGGEGSFSPRLGFQFDADART
                     RLRAAYAPGSDAGMRVEQLTLGEDSDVLFKELAGQAVAILDGRAVAERSRRLEFSIER
                     VIDENSTVEATAFFDTVDGRGVGLLGMPLGAFAAGGGDDAALADVANQQGGARGLRVV
                     YTRRVGQRLKATAAYAVGRGQALSEEALTSPEQLFQDAIFQTAAAQLDADVRDGTRVR
                     TVLRFSPRAAVFAIDPFAGRLAVYDPSLSILVTQDLPNFGLPVRAEATLDARNLLDLL
                     SSVEDGEGMLSVNAMRRMVRGGISVRF"
     gene            6079..7383
                     /locus_tag="VF240_14815"
     CDS             6079..7383
                     /locus_tag="VF240_14815"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7176535.1"
                     /translation="MTEAIRTTRLPNGLTVLSEHMPSLRSATVGIWVRRGSRHESAPL
                     NGICHFIEHAVFKGTRRRTALDIAVESDRLGGNLDAYTTHELTGFAMKVVDSALPQGF
                     DLIADMLSAPRFDEEDLRREQKVIIEEMKMVEDTPDELLMELFSARYFPEHSLGRPIE
                     GTAETVSTFDSQRTTRFHERAYAPRNLVVAAAGNVEHDALLELAARLFGDAAADTVDD
                     KDAPEDAPRAEGDLSAPAPAAPIFVERKKELEQAHMILATPWPSSRDEDRYAGTLLAS
                     IIGGGTSSRLWQSVREERGLAYSVGAAASHFTDAGIFQIYAGTSPEHLDEVLDLSLAE
                     LRRILREPVSEDELRLVKDQAVSSILLGLESTSARAGTLARQEIVHGQRISPDEIIRR
                     LEAVTPEDLLRLARTYFRADSLALGALGDLNGFKVDRARLEM"
     gene            7925..8365
                     /locus_tag="VF240_14820"
     CDS             7925..8365
                     /locus_tag="VF240_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7176536.1"
                     /translation="MAIRKVTFDTNVFVDYDAAYFPAGFLMSAVVMQELAAGALDTSR
                     LKELEIARRRYLKEGRLLVPDSEDWLQAGKILNSMYRGTATNNRGRRVKIDRETQRRI
                     LRDVLIARTALMAKATVVTADVSDFEMIQAYCSVRVEAAANFFA"
     gene            8412..9623
                     /locus_tag="VF240_14825"
     CDS             8412..9623
                     /locus_tag="VF240_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176537.1"
                     /translation="MKAFTCPQCGASLEYERIVTVTVKCHYCNSVVVVPVDLRPPPPP
                     TPPRPEREPLNSFGDSRPNKTVPALIVALVLIGGLSLLIVGLSRSSSNTNRSTGLFST
                     PTPRRTPTPVPTPDPGYTVAFTFGAEGTGPGFFKDEMDVAVDGEGRVYVSDDTRRVQR
                     FDASGNFLGTWTIPSETKWYKRLRGGPDKLLAPRWGSQVYAVLAGVVAKFEGATGEVL
                     GAAHGSDYVHDATLIPDGGLLMVTQKGDDDELVLMGGDGRVSRRTHRFVSSLLDKQLT
                     VKALRVAADGTGNTFALYALGDVYGEHWYDDDDLAVFRFTPEGKYVSRFGSGGHEPGQ
                     FGVPSAIAVDNQSRIYVCEPFNKVHIYADDGRHLSTFEAPHMVKALTFDAANHLYIAG
                     GHKVSKLVLTR"
BASE COUNT         1737 a         3176 c         3234 g         1566 t
ORIGIN      
        1 tcgctcgcgg cctgaaggcg ggcgcgtgtg gccgcggtcg ggagtgtgat gcggaacctt
       61 gtgccgcgcc ccggcgaact ctcgaccgag atgtgaccgg cgtgctcctg cacgatgccg
      121 taggagacgg cgagacccaa ccccgtcccg cggccgacgc ccttcgtcgt aaagaacggg
      181 tcgtagatac gcgcgacgtt ctcggcggcg atgccgatgc cgtcgtcctc gacctcgacc
      241 gtcaccacgt cgccctcttc gtcggcgcgc gtgcgcaaag tcacggtgcc gccgtcgggg
      301 atggcgtcgc gggcgttcaa gaggaggttg gtgaagacct gctggagctg gcccggattg
      361 ccgaacacgc gcggcacgcg ggggccgtac tcgcgcctca cctcgacgtg gctgtggcgc
      421 aactgtgttt cgagcaactg gagcgtgtct tcgagcacgc ggttcaagtc aacctcggcg
      481 aagtccgtaa cgccgcccgt gcgcgagaag ttgagaaggt tgttgacgat ccccgtagcg
      541 cggtcggtct ggcggcgaat cttttgcaag agcgcgtgct tcgggtcggt gtcggccacg
      601 gtgccgagga gcatctgcgt gtaggaggag acgcccgtca gcggggtgtt gatctcgtgg
      661 gcgacgccgg cggcgagcag gccgatgctg gagagttttt cgcgctgctg caactgttct
      721 tcgagccgga cgcggcccgt cacgtcttcc aagacgacga gcgcgccggt gtggtcgggc
      781 gagtcggggc gcttgagggg cgcaatcgag atgttgagga tgagcgagcg gccctgccgc
      841 gcggcggtgt gaagcttgta gatgtggcgc acttcgccca agcgccagcg cgagacgccg
      901 agcacctggc cgagcgtctc ggcgaagtct tcggcgaaga ggtcttcgac gcgcgcgccg
      961 aacgcgtcgt cgcgacgcac gccgagcaat tcttcgagag aggtgttgca ggccgtcacc
     1021 cgcccctcgg aatccacggc caccagaccc acattgatcg actcgacgat ggactcgttg
     1081 aactccttca aaagcaccaa ctcctcggcc cgctcgtgct gctcctgata gagcaggctg
     1141 ttctcgatgg cgacggcgac gtagcccgag acggtgcgaa ggatttccag gtcttccgag
     1201 gagagcaggg cgcctcccac cgagcggccc agcccgatga cggcgaccat ccggccgcgc
     1261 acgacgcacg gcacgtagta gtgcagcgcg cggcggacga acccgacggc ctcgggggcg
     1321 aggtcgatgt cgtcagcgcg cacgatgccc tcctgcgccg agcgcgtgcg gatcatctcg
     1381 cggaagtccg gcggcacgat caacccgctc gccagccccg cggctcgcgc cacgcgatag
     1441 cccgacgggg cgcgcgcgtc ctcgatgaag atggcgatgc gctcaactcc cagaacttgc
     1501 tggaggcggc tgacgagcgc gtctaagagc ggctccagcg tggtcgtcgc cgagagcgtg
     1561 cggccgaagt cgaggaggcc gtggcgcagg tcgtagcgtt cgccgtagaa gaagcggtcg
     1621 atgcgctctt gcaggaaatt cttgagcggc ccggccgtca tcacgatgag ggccatcgcg
     1681 gccgtggcga cgatgaactg gagcgtggcc acgcccggcc cgacgccgcc gcccttgagc
     1741 gccaccagat caaggcccga agccttgagc accacgagat agatgaccgt gccgatggcc
     1801 agcgccacgg cgagcgtcgt cagcgcgcac gatgccctcc tgcgccgagc gcgtgcggat
     1861 catctcgcgg aagtccggcg gcacgatgag cccgctcgcc agccccgcgg cgcgcgccac
     1921 gcgatagccc gacggggcgc gcgcgtcctc gatgaagatg gcgatgcgct caactcccag
     1981 aacttgctgg aggcggctga cgagcgcgtc taagagcggt tccagcgtgg tcgtcgccga
     2041 gagcgtgcgg ccgaagtcga ggaggccgtg gcgcaggtcg tagcgttcgc cgtagaagaa
     2101 gcggtcgatg cgctcctgta ggaaattctt gagcggcccg gccgtcatca cgatgagggc
     2161 catcgcggcg gtggcgacaa tgaactggag cgtggccacg cccggcccga cgccgccgcc
     2221 cttgagcgcc accagatcaa ggcccgatgc cttgagcacc acgagataga tgaccgtgcc
     2281 gatggccagc gccacggcga gcgtcgtcag cgcgtagacg gccgtgcggc gcacgaccat
     2341 gtccacgtcc atcaggcgat agcgcacgac cgagttgccg aaggcgagcg ggatgagcac
     2401 cagcggcagg atggccgcgt cggtgagccg ccgcgccgcc gactcgctca gcacgctgga
     2461 gaggcccacg cccgagaggt aatcgacggc gtagacgagc gtgaagggcg tgacggcgac
     2521 ggccgaaccc cacacgaccc acttcaactg ctggcgcacc acggccgact cgctacgcag
     2581 gaagcggtag acgaggacgc ccgacccggc ggcgagcgcc accatcaggc agacgaactc
     2641 ggcctgagag agccacccgg cgaagccgtc gggcaacccc gtgtaggcgg cgagcagcgc
     2701 gttgtagatg gaggcgggca gcaccgcgcg caggtagacc gtgaagtcga agaaggccgt
     2761 gagcgcgatg agcacgaggg ccggcacata gagcaacgcg gccagccagc gccgcttgtc
     2821 cgagaggcgg tggcgcacgg gatagatggc gcagaagtgt aggaagagcg gggcgaacag
     2881 ttgcaacgcg gtgtggtcta aaaaatccac cgcgaggtca acgtccttga agttgccgaa
     2941 gggcttgtag aagtggaaga cgaaggcggc gaggcagagc gccgtgaagt gcaggacgaa
     3001 gggcgcgcgc tcgccctgct tgaagaagac gaagaggccg acgagcaggt agacgatgcc
     3061 gacggcgttg acgtagaggt cgcgcgtcgt ccaggtcgcg agcggcgtca ggttttcgag
     3121 atagacgtag tagaagcgcg tgtcttccgg gtaggacggc cgctcgatga ggtagcgcag
     3181 ttggccggtc gtgctcgact cgtcgatgaa gatttgcacg tcggagacgc gctcgaccgc
     3241 ctcgccgccc tctcgcccgt cgctggtgct gatggcgatg agccggtcgt cgggcgcgat
     3301 gccgaagacg cctttgcggc ccgcggctga gtcgggggcg actgaggcgg cgacgatccg
     3361 gccgtctcgg gtctgcaccc actcgacacc gtcggtgagc ggcggggtgt gcgtcaggcg
     3421 ctgggcgaag ttgagggtgc cggccgcgac gagcaccgcc gccgcgatca ggagcaccca
     3481 agccttcgtt gtcagcaccg agcttttcat ctttcgtctc gacgcgcccg cagcactgtc
     3541 gattcaactc cctaaagccc tacttaaact aaagttatga agcaaggccc attccaatcg
     3601 gacggggccg tgcggaatgc tccgggtcgc acgaccggca gggatgcgcc cgcccgacgg
     3661 aagcgcattt ttacggcgtg attagcccgc ccgggcgcgc tggaaagtct tcctgatgcc
     3721 cggccggtcg cccgactttt caattcagtt tttcgtattt ccttccgaaa tttcggagaa
     3781 atagcgcatt ctaggcaaaa gccccgcgtc ttgcgcaagg cgcggggtgg gtgggccatc
     3841 gttcgtttaa ctgtctcatc agactcccga agcctcacga gaccgagggc gcgtgagagt
     3901 tcttaaaacc tgacggagat gccgccgcgc accatgcgcc gcatcgcgtt cacggagagc
     3961 atcccctcgc cgtcctcgac gctggagagc aagtcgagca ggttgcgtgc gtcgagcgta
     4021 gcctcggcgc ggacgggcaa gccgaagttg ggcaagtcct gcgtgacgag gatgctcagg
     4081 gaagggtcgt agacggcgag ccggccggcg aacgggtcga tggcgaaaac ggcggcgcgg
     4141 ggcgagaaac gaagcacggt gcggacgcgc gtgccgtcgc ggacatcggc gtcgagctgc
     4201 gcggcggcgg tctggaagat ggcgtcctgg aaaagttgtt cggggctggt cagcgcctct
     4261 tccgacaatg cctgaccgcg cccgacggcg taggcggccg tggccttgag gcgctggccg
     4321 acgcggcgcg tgtagaccac tctcaggccg cgcgcgccgc cctgctgatt ggcaacgtcg
     4381 gcgagggccg catcgtcgcc gccgcccgcg gcgaaggcgc cgagcggcat gccgaggagg
     4441 ccgacgccgc gcccgtcaac ggtgtcgaag aaggcggtgg cctcgacggt cgagttctca
     4501 tcaatcacgc gctcgatgct gaactccaga cggcggctgc gctcggcgac ggcgcggccg
     4561 tcgagaatgg cgacggcctg accggcgagt tccttgaaca gcacgtcgga gtcctcgccg
     4621 agcgtcaact gctcgacgcg catgccggcg tcggagccgg gagcgtaggc ggcgcgcagg
     4681 cgcgtgcgcg cgtcggcgtc gaactggaag ccgaggcgcg ggctgaagct gccctcgccg
     4741 cccgcgccga gaaagcgcga gtagtcgaga ccgaggacga cgaccacacc ctcacgcaca
     4801 acccactcgt ccgtggcgcg cacggagagc tgttcgagca tgtcgtcgcc gagcaggagc
     4861 gcgcccttgc ggcccagagg cagacgcgcg ccgcccaccg tcgcgctcag gcggtgatga
     4921 gcgccggcac gcacgcgcgc cgtcgcctcg aagcggtcga acgcgccggc ctgtccgacg
     4981 aagacgagat cgacgttggg gctgacgggc gtgagcgcgg cgaagttgat gctcgcgaag
     5041 ccgccgtggt cgtcgccggg cgaggccgcg tagaaagtct ggacgacgcc gtgcggccgg
     5101 ctcgcccaga agcgccgcgt gtcgatgtcg gccgtctctt cggccatcgt ctcgcctgtc
     5161 gccgtggccg tgtcggtcgt gccctcggcc gtctcgcccg tggccgcgcc cgccgactcg
     5221 ttcgcgttga agatgtggcg gcggcggaac gaggcgcgga ttttatactt cgggtcgtcg
     5281 cggtcggcgc ggcgctcggg cgtggtgagt ccctgacccg tgggttcgag gttgaagcgg
     5341 tagacgagtt ccgcggaagc cttaacggtg actgcgggga aggtcgtcgg gctgaatcca
     5401 gaggctacgg cgcgcaggac gtatgtgccg ggcgcgaccg tcgccttgta ggtgccgtcg
     5461 gcggcgctac gcacctcctt gacgattcct tcggcgccct cgcgcagcag cgagatgacg
     5521 gcgccggcga gcggattgcc ctgactgtct ttgaccgagc cggtgacggt gccgacgggg
     5581 cggcgaccac cgccaccggc cgcgaaggct gaaggcgcgt tctcgccgca cgcgacgaag
     5641 atcgcgagca gcaccagccc cgtccgcatg taggattttc tcatcggtgc caaacctccc
     5701 ggcgcgcccc ggcgcgcaag cgcaggagac gccctctcca cgtcacacat cttttccttc
     5761 tgttcttttc agacggcgtc catgaacgag gggttacgcg cctttacggc tttacggctg
     5821 cgacgccaag aacgagggac acgggagacc gggtgtccga cccgcttgaa cgcaagttag
     5881 gcgcattttc cgccaacatt cgcaggctgt caagcgggcc gggcgctcca aaccgtccgc
     5941 ccgccggtct ttcgtgtccg ctatgatgcg ccccgagcgg ggccgggttt gcccgcgccc
     6001 ggccggcgtg ttacgattag ctttgttaag aaaacctttg atttccgcca tttttcaccc
     6061 aattttcaac agaagcaaat gaccgaagca atacgcacca cccgactccc caacggcctg
     6121 accgtgctta gcgagcacat gccgtccctg cgctcggcca ccgtcggcat ctgggtcagg
     6181 cgcggctcgc gccacgagtc ggcccccttg aacggcatct gccacttcat cgagcacgcc
     6241 gtcttcaagg gcacgcgccg ccgcacggcc ttagacatcg ccgtcgaatc cgaccgcctc
     6301 ggcggaaacc tcgacgccta caccacgcac gagttgaccg gcttcgccat gaaggtcgtc
     6361 gattcggccc tgccgcaggg cttcgacctc atcgccgaca tgctctcggc cccgcgcttc
     6421 gacgaggaag acctccgacg cgagcagaag gtcatcatcg aagagatgaa gatggtcgag
     6481 gacacgcccg acgagctgct catggagttg ttctccgcgc gctacttccc cgagcactcc
     6541 ctcggccgcc ccatcgaagg cacggccgag accgtctcca ccttcgacag ccaacgcacc
     6601 acacgtttcc acgagcgcgc ctacgctccg cgcaacctcg tcgtcgcggc ggccggcaac
     6661 gtcgagcacg acgcacttct tgagttggcc gcgcgtctct tcggcgacgc cgccgccgac
     6721 acggttgacg acaaagacgc gccggaggac gcgccacgcg cggagggcga cctctcggcg
     6781 ccggcgccgg ccgcgcccat cttcgtcgag cgcaagaagg agcttgagca ggcgcacatg
     6841 attctggcga cgccctggcc ctcctcgcgc gacgaagacc gctacgccgg caccctgctc
     6901 gcctcgatca tcggcggcgg cacctcctcg cgtctctggc agtcggtgcg cgaggagcgg
     6961 ggcctcgcct actcggtcgg cgcggcggcg agccacttca ccgacgccgg catctttcaa
     7021 atctacgccg gcacctcgcc cgaacacctc gacgaagtgc tcgacctctc gctcgccgag
     7081 ctgcgccgca tcttgcgcga gccggtctcg gaagacgagc tgcggctcgt caaggatcag
     7141 gccgtctcat cgattctcct cgggctggaa tcgacgagcg cccgcgccgg cactctcgcc
     7201 cgtcaggaga tcgttcacgg ccagcgcatc tcgcccgacg agatcatccg ccggctcgaa
     7261 gccgtcacgc ccgaagacct cctccgcctc gcccgtactt acttccgcgc cgactccctc
     7321 gccctcggcg ccctcggcga cctcaacggc ttcaaggttg accgggcgcg actggaaatg
     7381 tgaaaagctg tgaatcgtaa atcgatttgc tgaccgccgt ctgactcgtg gattgccgct
     7441 caagccggat acctctcaac gacaattcac aatttacggt tcaagattta tgattcaaca
     7501 gagggcaaat acgagcaacg tcttcacgga ggttaatcgc atggcattgc aaaaaaaggc
     7561 gacggcaaaa agccccaaga acggtcgagc tgcatcgacc aaacacacgg ttgtacggtc
     7621 gagcgttacg gggaggcttg tagttgcccc aaagtcaggc ggttgggcag taaagcgtga
     7681 aggcggcaag cggactcgtg tctacaccac gcaggcagac gccattaagg ctgccactaa
     7741 gactgcgcgc gacagcaaat ctggtgttgt aattcatggc aaggacggac ggatcaagca
     7801 ggtatcgaca tcccgcgcag actctctaat gctcgatgtg tggaagtccg cgcgtcaagg
     7861 ccaggggggt tccaagaatt ccaagggcag ctctaagagt tattccaaga atggatagcg
     7921 cggtgtggcg ataaggaaag tcaccttcga cacgaacgtg ttcgtcgatt acgacgcggc
     7981 ctacttccct gccgggttcc taatgtcggc cgtcgtgatg caggaactcg cagccggcgc
     8041 gctcgacacc tctcggctaa aggagcttga aatagcccgg cgccgttacc tgaaggaagg
     8101 gcggctccta gtacccgata gtgaggactg gcttcaggcc ggtaagattc ttaactccat
     8161 gtatcgagga acagcaacca ataatcgagg gcgccgagtg aagattgaca gggaaacgca
     8221 gcggcggatc ctccgcgatg tcctgatcgc gcgcacggcc ctcatggcaa aggcgaccgt
     8281 ggttaccgcc gacgtgagtg attttgaaat gattcaggcg tactgttccg tgcgtgtaga
     8341 agccgccgcc aactttttcg cataagccaa caacgtccaa acgtctggag attcacgatt
     8401 cacggcttct catgaaggcg ttcacctgcc cccagtgcgg cgcgtcgctt gaatacgagc
     8461 ggatcgtcac cgtcaccgtt aagtgtcact actgcaactc ggtggtcgtc gtccccgtgg
     8521 acttgcgccc gccgccgcct cccacgccgc cgcgtcccga acgggagccg ctcaactctt
     8581 tcggcgactc gcgtcctaac aagaccgtcc cggccctcat cgtcgcgctc gtcttaatcg
     8641 gcggcctctc gctgctcatc gtcggcctca gccgctcgtc gtcaaacacg aatcgctcca
     8701 ccggcctctt ttcgaccccc acgccgcggc gcacgccgac gcccgtgccc acgcccgacc
     8761 ccggctacac cgttgcgttc accttcggcg cggagggcac cggccccggc ttcttcaagg
     8821 acgagatgga cgtggccgtt gacggcgagg gccgcgtcta cgtctcggac gacacgcggc
     8881 gcgtgcagcg tttcgacgcg tcgggcaatt tcttgggcac ctggacgata ccttcggaga
     8941 cgaagtggta caaacgcctg cgcggcgggc ctgacaagct cctcgccccg cgctggggca
     9001 gtcaggtcta cgcggttctg gccggcgtcg tcgccaagtt cgagggcgcg accggcgagg
     9061 tcttgggcgc cgcccacggg tcggactacg tccacgatgc gacgctcatc cccgacggcg
     9121 ggctgctgat ggtcacgcag aagggcgacg acgacgaact cgttctgatg ggcggcgacg
     9181 gccgcgtctc gcgccgcacg caccgcttcg tcagctctct gctcgataag cagttgacgg
     9241 tcaaggcgct gcgcgtcgcg gccgacggga cgggcaacac cttcgccctc tacgcgctcg
     9301 gcgacgtgta cggcgagcac tggtacgacg acgacgatct ggccgtcttc cgcttcacgc
     9361 ccgagggcaa gtacgtcagt cgcttcggca gcggcggtca cgagccggga cagttcggcg
     9421 tcccctccgc gattgccgtc gataaccaaa gccgcatcta cgtctgcgaa cccttcaaca
     9481 aggttcacat ctacgccgac gacggccgcc acctctcgac cttcgaggcc ccgcacatgg
     9541 tcaaggccct gaccttcgac gcggccaacc atctctacat cgccggcggc cacaaggtct
     9601 cgaagcttgt gctcacgagg taaaacggtg ataagtgatg agtgataagt gatgagtgat
     9661 aagtgacggg tggtgagtgt ttagtgacaa gttgaagagc atgtcttaaa ctt
//
LOCUS       DASVES010000363        37349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1433207, whole genome shotgun sequence.
ACCESSION   DASVES010000363 DASVES010000000
VERSION     DASVES010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 37349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37349
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433207"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF240_14830"
     CDS             complement(<1..318)
                     /locus_tag="VF240_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176538.1"
                     /translation="MKSEMRIGVASLALLLALSPTAATAAAQKESKEPPKQSPQGEVK
                     AAHPAPTPGERLEPDDATVARPVASKEMLANRIGQRSEEEAAVLPFYNNFLTQYRLGP
                     EDVI"
     gene            complement(367..1755)
                     /locus_tag="VF240_14835"
     CDS             complement(367..1755)
                     /locus_tag="VF240_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEX7176539.1"
                     /translation="MSSTRFNTRTPLLIAAEALLLFGATVAAAYVRLGMEDAHFELTV
                     KHGYLKAALVGLFCLAAFYLYDLYDFVVMHDRRELVLRLIQALGLAWVALAILFYAVP
                     PLMVGRGVSLISLPLALTLMVGWRVAIHWLLGHPNVGERILIVGSGESAIEVAREALE
                     RRDAGFRIVGFVDNRPELLGQSLVNPRVIGLTKDLAEVVRRESVDRIVVAMGERRGQF
                     PVEELLDLSLSGGVAIEECASFYERLTGRVSLDMLRPSWLIFSGRGRQARLSSAARTF
                     LHRGAALVGGLLSLPLALLTALLVKLESPGPALYRQERVGKNGRVFTIMKFRSMRLDA
                     ERDGPVWASKAGDERTTRVGRVIRKIRVDEIPQFWNILKGEMNFVGPRPERPHFVSQL
                     AEEIPFYEQRHLIAPGLTGWAQIKYPYGASIEDARQKLQYDLFYIKNQSLMLDAVILF
                     ETVKTILFGRGT"
     gene            complement(1902..3029)
                     /locus_tag="VF240_14840"
     CDS             complement(1902..3029)
                     /locus_tag="VF240_14840"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX7176540.1"
                     /translation="MGRVYDALKRAAETNGAKGQGSGGDEQSRGGATERAAGNGANGH
                     GAGGDAAHASASSVVAPLPAPEQLLGRSAHITAPGSAPAGSAPNERTGRPVGSALPDM
                     QASRVAGATLGAAGSMRADAFQSLEVTSARVEPHIIAVTQPNSPHAERFRSLRTRVLH
                     AGERKKMQAFVITSAGIMEGKTMTAVNLSWLLAQTDGVRALLIDADLRKPTAGDYLGI
                     DAPFGLSEVLAGEATLEESIIRLTPAGLHLLPGGEPRDNVAELLSGPRFGRVLKELRG
                     LYDYIIIDTPPLGIFTDATLVISRADGVLVVARAGRTRFSALERLLEPIPRERVLGIV
                     LNGADEPPGENYNNNYYYRRPRYQRRDEAPAETAEVVEEKA"
     gene            complement(3031..4110)
                     /locus_tag="VF240_14845"
     CDS             complement(3031..4110)
                     /locus_tag="VF240_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7176541.1"
                     /translation="MYLDFYGLKELPFALTPDPRFIYFTPSHTEAMANLHYGIESGKG
                     LIVVTGEVGTGKTTILRWMMQRLDRTVLVGYIFNPRMSVEEFYQHIASSRLLNVQSWH
                     NKSELIIALGDTLEERHARGLRTVLIVDEAHGLSTAVLEEIRLLSNFESDTAKQLQIV
                     LTGQPELREVLNRQELRQLKQRVALRCEIRPLPDVEETERYISSRLLVAGAERTDIFT
                     ETAIDFVFRCSNGIPRLVNNLCDNALLSGFAAGAETINRAIIEEVADTFDLLPTASRG
                     RQTGEERESQSRIFTAQGRAELWAAGAGTDEGRGPMAGAAHAHEEREMERELERELGS
                     DATAVADSGGDLGRASDGWGREGGK"
     gene            complement(4207..5832)
                     /locus_tag="VF240_14850"
     CDS             complement(4207..5832)
                     /locus_tag="VF240_14850"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX7176542.1"
                     /translation="MSVELRQRKPAEYARMLWRRKWMIMLPAAAITLAVGWAVWKLPN
                     IYESTTLLTVRPPSISPSIVPQLTGDDLTIRINSIGQEVVSRSSLEPLILKFNLYAAE
                     RQRDEPMDVLVELMKTRDLAVKINTTRNDITNGFYLSFRSSDPKAAQAVAAELASKYT
                     KAQTVASTQDATATRDFFERRLQESKQQLDEIDRRRLQFMMQNVTHLPSHSQALVGQL
                     AGLREEQKSLNTSLDFLRQRRSSLVSQGGEFEKRRAEEIDLFIDNIQDPKTTPAYASL
                     VARREQLRSDKDRMLTELRPKHPDVVMKQTELDNVQREIDEMVADYKQRIEDRRKRLE
                     GASDPRQHSMKEELQKVDGDMTVLQKRLALNDQQTAEVNRRLGGMPTAEVGLESINRD
                     YQTAKTVYDELLEQSEKAKLISDVTSNSQGESIEVIDAASLPQQPVAPKRPLLMFLGL
                     VAGLCTGLVFAAAFEVPKLLTIQNREDAEHYTGLPVLVTLPQMLTPREERRLRLRRVA
                     LTTAGLASAVLSVPALYAALRLTRLLEVFAFRG"
     gene            complement(5918..8389)
                     /locus_tag="VF240_14855"
     CDS             complement(5918..8389)
                     /locus_tag="VF240_14855"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176543.1"
                     /translation="MRFFECLRARGFAPALLAVLLACAVLTAACSDPEKLKAEHVTRG
                     EAFLKERKFHEAALEFRNAIQIDEALASAHWGLARAYEGLERFNESFDEMNRALKLDP
                     SNKEARYKIGSYLLIGSQRGSKVTNPELLEEAGIHAEEILKQDANYVPAHILLANVLA
                     ARNGPNDRVEALKKINHAIELDPARVESYISLAGFYRLVGDLGEAEKTFKRAIQLNDR
                     ASLAYVEYGKFLAQQRRTDEAEAQFRKAVEVDPTNQDVHWILASFYLLNNRLDRAEEA
                     YKALAALDPTRPDGRTKLADFYASTGRLDDALSVYRESLQLFPEYARAHYRVGELLLQ
                     KGDRPGAAAKAEEILKKNGRDPEALLLRARLRLEQGQTKEAIADLSGILEQEPRSQLA
                     LYFMSEAQFRDGQIEQARSFTGDLERFHPNFLPAKLMQLQVNLSGGDPEMVRRQATEL
                     LDRLRETPPSAQQTPQLLSEIKVRTLTARGMANMRLRGAAKNAGAAASLLAAARADME
                     AARDVWPNSPSSYTNLAGIAVVEGKYDEAFSLYERALTLDGTNFQALDGLVQLYGATN
                     RFQEARARLDALTAEHPGVAHLQYLKGTAYAQGTTQGVPPDAEATEAGFRRAIELDPD
                     FVAAYQKLAELYFNTNQPERSVAEYQKVTERRPNDAVTFSKIGMVEFSRRNLDAAAEN
                     YRRALAINPGEEIAANNLAMLYAEYEKGNLDEAVRLAQEVVRRRPEQPGYADTLGWVY
                     YKKGLYGPAVEQLQKAVSLSAATGGDNSVYRLHLGLALTGKGDRAAARRELLAAQRLA
                     QAEAVGPLRRPKAPVEDIQRALESL"
     gene            complement(8625..9275)
                     /locus_tag="VF240_14860"
     CDS             complement(8625..9275)
                     /locus_tag="VF240_14860"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEX7176544.1"
                     /translation="MKTRDRHFWVLVVLLVAGGAVVQFWERAGEARVPRSALAELPAS
                     LGEWSQRRADVRFDQATEAVLKADDYVLRDYALADGRMASLYVGYYASQRAGATYHSP
                     LNCMPGSGWILSDPAVIRVQPADGSAAFEANRYVIQNASERFALVYWYQGRGRAVASE
                     YWDKLYTAWDSMRSRRSDGAMVRVIVPMRRSEEEAMNLAAELAARVSPELPRFVPN"
     gene            complement(9288..10331)
                     /locus_tag="VF240_14865"
     CDS             complement(9288..10331)
                     /locus_tag="VF240_14865"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEX7176545.1"
                     /translation="MQTTERISFRPLLIAGAIAFAYWGVLAKLGHFWWTDENYSHGLL
                     VPFIIGYILWSERTRLARDAGRPWPALGLAGAACALAALWAGVAGAELFVQRMSLVLL
                     LASVAVYFWGGRILRVVVVPLLLLALAIPIPTIVFNKVAFPLQLFASRCAVWAMRAFD
                     IPVLREGNIIEFLPLNSMTTKKLEVVEACSGIRSLMTLVTLAVVYAYFTSPKSDGGDD
                     GGNSRSWYARNFKVLRAVLIVLAAVPIAILTNAGRVSGTGVLARYYGTEVADGFFHSF
                     SGWVVYVVAFLLLFAFGWLIDRFDPGRGRPRGGGSSAAATEEGAKADTGSDGKGVTVG
                     APSAAMTGGGEAV"
     gene            complement(10681..13374)
                     /locus_tag="VF240_14870"
     CDS             complement(10681..13374)
                     /locus_tag="VF240_14870"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7176546.1"
                     /translation="MTNLRLAIASRRSFSLALTAAALFILCLSATAQVRTDMGVYAEG
                     PTLTPPPVGGKFIDSLSGVEVMRLTDGANCPAGCGTYYSYWPTSNSDNTRVLVGNYGS
                     PAFLLRFDPVNFARGVREELPMEPTRGTPFWEGAVWSRTDPNTLYCTVGAAILAYHPD
                     TRRYDLLADLTAQLPGEYMIQTSFSRDGNRVSFTRKRSSDYAPVGFGVYDRALGRLML
                     SVLRTDMDETTLSGDGRWVVVKTNKQGPGTVEQLVYNVETGVSDPIIDDRDGAPGHGD
                     AGTDTYVGYDNNTNRMNWRRLSSPHAAAPLLTLPDWTQAIHTSMLADDGGWALVGLFG
                     EAPAPAPAPLRKELIQVATDGSGLVRRLLHHRSVYRKYEDSPRANISYDGRFVFYTSN
                     MGGTRTDLYVAKITPPGAAPAPSPTPSPTPAPTPTPTPTPTPTPTPDAGALMATVTSP
                     LSGSQFNAVANLTVTATATNTDAVVTKVEFYSGATLIGTDTTAPYGCAWNNVAAGNYT
                     LTAKATDSKGRSVVSNGVTVQIKRSTGSVGRAKKNAQTTSNQLSDLTGSSSSSVEGGG
                     ISTAQSSVVPKLDAVASDIQQAYGDFVEESGSYAAAGKIDGELQNALNYARLATGLAR
                     DGNLKDAKGALRVAIDHLEFANVYMAHGDVPNPAAVTEFFVRQHYVDFLNREPDDSGL
                     AFWTNEIASCGSDAQCVEVKRVHVSAAFFLSIEFQETGFLIHRLNKVSYGRMPSMSEL
                     QAGAAEIGQGVIVGAPGWRQKLEANMAAFYREWVRRRDFGAKYDSLTNAQYVDAIINN
                     AGVTLSPEEHHSWVALLDAGTERASVLRQVVERKEFVQSETIPGFVMMEYMGYLRRDP
                     DPVGYNFWLVKLQNHGGDFVAAEMVKSFIVSGEYRGRFGQQ"
     regulatory      complement(13387..13472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      complement(13820..13932)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            14185..14847
                     /locus_tag="VF240_14875"
     CDS             14185..14847
                     /locus_tag="VF240_14875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7176547.1"
                     /translation="MNKLSKVISCITLVFLLLAASEARADTVEITSGFVSIGGVYPPG
                     RGTFRTINFSLAGDNFTFNGTEVDGTTQQVTTCTFGPCPAGTLINSSSVVRLQGVGSA
                     TINGLFYPITQPLGSLLTFTGESISIPSSGEPVIFITTPFTMTGTLNVVVPPGTSVFS
                     ATAVGSGFATLTLRQFESGYVLTTIRYDFQRPIPEPSTIILLCTGLAGVVRVTRRRRS
                     KI"
     gene            complement(15055..15744)
                     /locus_tag="VF240_14880"
     CDS             complement(15055..15744)
                     /locus_tag="VF240_14880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7176548.1"
                     /translation="MASEYDKYVSAEWEMFVGDASRARESLEAVAGLDVGRVLDLGCG
                     AGQELLPFATELGASCVGVDVTPEVGLVGRRLFAANAPSAPVTFVRAAAELLPFDEGS
                     FDVVICRLALPYTDNARCLSELARVLRPGGALLLKIHHARYYTRKLRDSLRAGELPSA
                     VHAVRVLAAGLIYQLTGRQPRMRLPSRETFQTVTLLRRELARRGLEIQRELRDSNPLT
                     PSFLISRHAPR"
     gene            complement(15989..17515)
                     /locus_tag="VF240_14885"
     CDS             complement(15989..17515)
                     /locus_tag="VF240_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7176549.1"
                     /translation="MKKALIASLSVAAALALVYLGYTFAQHQVARPRVVNPGAKTITV
                     RPGDNLQRALDAAASGDEVVLSSGATYTGNFVLPVKAGDAFITVRSARCGELPEGVRV
                     SPDSAPLMARLATPNVSPVLRAPAGSHHWRLRCLEFTQGAAMKGEGYNLIQLGEGASQ
                     ENQKTLDDAPHHLEFDRVLVRARDDQTAVQRGITLNSASTSVTNSHISGIKWAGVETQ
                     ALGGWNGPGPFLIENNYLEAASINILFGGAAPTIPNLVPSDIVIRNNTLYKRPEWRGR
                     GYALKNLFELKNARRVRVTGNVMENSWADAQTGWAVIFNTFRDAGWEVVEDVTFSGNV
                     IRNVTNGVNLRGLDTGDTRVRLRRVAVSDNLIELGAHGAGARAFQVLNGSEGVSFDHN
                     TVRGAVSIALSLDTAEGFRHADLSFTNNLLPHGEYGVFGNGGAIGTAALNRFASAWAF
                     VGNALYARPSGAWPPYPPGNFFPGDEAGTAGLKGADGLPVGVRAQTPAPAPTARRKAD
                     "
     gene            complement(17583..18779)
                     /locus_tag="VF240_14890"
     CDS             complement(17583..18779)
                     /locus_tag="VF240_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176550.1"
                     /translation="MSENDAGLLAKLSWSLPWLARYPFWRAGAAARRAREGGAPLHLI
                     VVVANHFEPAWSERGGFLPLDEQRRRLDQWCETARATGAAVRDHDGTPYRHTNFYPAE
                     QYHAPILDTMAGLQAEGFGEVEVHLHHGVETPDTEAGLRRALVEFRDLLAEEHGCLSR
                     ETPGGPPRYAFVHGNLALANSAGGRFCGVDNEMRVLAETGCYADMTLPAAPLQPQVPR
                     LNAIYECGRPLEEAVPHRTGPSLRVGRRPQLPVILTGPLVFNWSRRVRGLPVPRLDDG
                     VMAANYPSDFERLERWAGARISVLGRPEWVFLKLYCHGFFTHDQDACIGEGARRFWSE
                     ALEESARTGRYKIHFASAREAFNIAAAAVDGRQGEPGQYRDYQLRPIMNEQAAQPTPA
                     RDALAM"
     gene            complement(18776..19627)
                     /locus_tag="VF240_14895"
     CDS             complement(18776..19627)
                     /locus_tag="VF240_14895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7176551.1"
                     /translation="MSSHAREVARGERFEFGDNWSRFLAALDDGRIREAEESLRLMLG
                     VESLAGRSFLDIGSGSGLFSLAARRLGARVHSLDYDPRSVACTAELRRRYFPEDPAWR
                     VEEGSALDADYIRSLGLFDVVYSWGVLHHTGQMWLALENAQLPVKPGGRLFVAIYNDT
                     GSQAARWKWIKKTYNRLPRFLRPPFAALAVAPDEIKPALRSLVTLRPQEYVRRWTDYD
                     RNRGMSRWHDIIDWVGGYPYEVATPDEIFDFYRARGFTLTRLKCGGVGLGCNEFVFVR
                     DGGAQTT"
     gene            complement(19652..20314)
                     /locus_tag="VF240_14900"
     CDS             complement(19652..20314)
                     /locus_tag="VF240_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7176552.1"
                     /translation="MSSAARQRGVKFGRAGRRRQAWAVVAVLVIAWPPVAWAAALALV
                     ETAGIARADALVVLSGSAAYVERSRRAAELYHARRAPRVILTNDNRRGGWSEALQTNP
                     LFVERAREELLRAGVPAESIEVLPEAVAGTHDEALAARAYAERAGARSLLFVTSAYHT
                     RRALWTLRRAFRGSGVEVGVEAVEEGAQTPPAATWWLYRSGWGAVALEYPKLLYYYWK
                     YR"
     gene            complement(20307..21350)
                     /locus_tag="VF240_14905"
     CDS             complement(20307..21350)
                     /locus_tag="VF240_14905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7176553.1"
                     /translation="MIGPYSPPRGGVQAHVTALREYLLGRGVACEVIDLSPNRRDEGA
                     GVYGPSGGLGVARLLLTLRYDVAHLHVGGNLSARLAALLLLCGSLPGRRTVLTFHSGG
                     YPSSPEGRAARPRSLRGLALGRLDRVVAVNEEVAAWFRQVGVEARRVRLISPYALPAA
                     PPAVQPPGPLRSFFAGHRPVLVSVGGLEPEYDLPLQIEALGRLREKFPRAGLVLVGSG
                     SLEEDLRRSVAAKSYGEHVLVCGDVERAVTLRVIADSDLMLRTTLYDGDSIAVREAVH
                     FGVPVVATDRAPRPAGVRVVPAQDLARLCAAADECLREGARRTPGDVLGFENLEAVLA
                     LYEELLASKSRDE"
     gene            complement(21388..22587)
                     /locus_tag="VF240_14910"
     CDS             complement(21388..22587)
                     /locus_tag="VF240_14910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7176554.1"
                     /translation="MSHSDSQSRGRPPASEQLRGRDIICFSHDWSGDPLSKTHLMRLL
                     ARDNRVLWVNSIGYRTPTVSKADAGRAFRKLAAAATPIRNPEPNIYVLSPLAIPAYGS
                     VAVRTINRHLLRLQVRRAMRKLGFRRPVNWVFNPPAAVVAGTLGEEQLIYQCVDEYTA
                     FTGVSGEVMAVFEEKLLRMADLAIVSSERLLQSRGKINPHAVLVRHGVDFAHFSRALD
                     PETKVPAEIASLPRPVIGFFGLVADWVDVDLMAEVAKHFKHGSLVILGKVTTDVSVLE
                     ALPNVHLLGRKPYESLPAYCKGFDVALMPFRLNELTLNANPLKVREYLAAGLPVVSTP
                     IPEVEVLGQCRIADGPRETIREVEAALKDPGPSRARSEAIRPESWEARLDEIRQHLAA
                     LAPRARA"
     gene            complement(22641..23429)
                     /locus_tag="VF240_14915"
     CDS             complement(22641..23429)
                     /locus_tag="VF240_14915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7176555.1"
                     /translation="MRPHGTAYLMYHELARPGRALCQDDPGYASYVVGESDFRGHLAA
                     LKADGFEGVSAGRALGDGGGAARRVAITFDDGCETDLVSAAPLLSEFGFGATFYVVAG
                     FVGGRGYLAPGQVRELAEQGFEVGSHSMTHRLLNGLADAEVRRELAESKERLEQWTGR
                     RIEHFSCPGGRWDARVAAAARAAGYRSVATSRVGANAPGGDPFRLARLAVRRGAGARD
                     VVRAARTESLFARRARAAVLDLAKGLLGDALYQKVHAKVGTRSK"
     gene            complement(23429..24223)
                     /locus_tag="VF240_14920"
     CDS             complement(23429..24223)
                     /locus_tag="VF240_14920"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7176556.1"
                     /translation="MLRGVIHTHSTYSDGEYTLSELREVLVAAGCSFACMTDHAEYFD
                     EERLRAYVAECEALSDEHFRFIPGLEYECERRMHVLGYGVTTLAGTTDPQKVIAHIER
                     AGGVSVIAHPMDSMFEWIETFEVLPRGIETWNSKYDGRAAPRPGTFKLLARLRERRPG
                     MLAFYGVDYHWKKQFRELYNLVECDAPGRADVLAALASGSYVGAKGDLRLPASGELPE
                     ALLGEFGDVNSRSMRRRRLIKKVKKVADRLGLKIPAPVKAQLRRIF"
     gene            complement(24195..25448)
                     /locus_tag="VF240_14925"
     CDS             complement(24195..25448)
                     /locus_tag="VF240_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7176557.1"
                     /translation="MRVLVLDGNENQAVACVRSLARAGHRVLVGSSASWSKAGWSRGA
                     SGTFRYPAPQADAADFVRRVAEEAAREPGTLVLPMTERTTLPLSERRGQIFEAGGRLV
                     LPPAATVRRAFDKQETTALAAALGVAVPRTALVSDADAARRVMREARYPVVLKPRSSE
                     EVSAAGGVVATGAPLYARDAGEFEGAYADLSRRASAVLVQEFVGGTGAGYFALMREGE
                     VRAEFAHRRVRDVRPTGSGSAVRVSVRPEARVREASLAILGALGWHGVAMVEFRIGPD
                     GTPVFMEVNGRFWNSLPLAVYAGVDFPALVAELAERGDVGRQAPYAEGVRCRWLLGDF
                     RHLIEVWRGAPAGYAGEFPMRAAALAAFLRPVAGTFHDNFAWRDPLPELGDWLDFAFR
                     RLPGGLKKLAGRREEVNAQRGYSHS"
     gene            complement(25521..27482)
                     /gene="asnB"
                     /locus_tag="VF240_14930"
     CDS             complement(25521..27482)
                     /gene="asnB"
                     /locus_tag="VF240_14930"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7176558.1"
                     /translation="MCGINGIALSSRSGRRVEAGLVERMRDVLTHRGPDDCGLFVEGS
                     VGLGHRRLAIVDVAAGHQPMTNEDGSLQIVYNGEVYNHADHRPGLEARGHVYATHCDT
                     ETILHLYEEKGAACVEDLRGMFAFAIWDARRRELFIARDRLGVKPLYYALTEDGSLYF
                     ASEIKALLEAGAVRAELNYAALPDYLANHAPSGEETLYRGVRRLLPGHTLLWRDGEVK
                     VERYWDVTFSDDGRGNEGRREADLIEEWSELFRESVRLRLMADVPLGMFLSGGIDSSA
                     IAAVMSKLVDEPIKTFSVAFNEREANELEYARMVSGAFGTDHHEVVVSPDEFFDALPL
                     LVWHEDEPLAHLASIPLYFVSRLAQRHVKVVLTGEGSDELLAGYARYRKTVYNLALGR
                     GYETLAPAPLRRAVARAVAGLPAGSRVRQKLGRTFLCLPADLESLYFDNFAVFPRAMQ
                     RRLLTPETAERAGSTDPYAGVRRLVEETDADSLLNRLLYADTKTYLHELLMKQDQMSM
                     AASIESRVPFLDHKLVEFSARLPERLKLRGWTTKYVLRRAMKGVLPEPILTRPKMGFP
                     VPFGAWLRGRHRGVLDEYVLGERARARGIFEADFVGQLASEHVAGLRDHSSRLWSLVN
                     FEVWLRQFIDGEQLFDAREARAEPAAVAV"
     gene            complement(27488..29995)
                     /locus_tag="VF240_14935"
     CDS             complement(27488..29995)
                     /locus_tag="VF240_14935"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX7176559.1"
                     /translation="MMKGALKKLKRLKGRGLGELRVRGAQALAARAERFGVGARLPSD
                     AGLARLLLPAPRGPESTDAESLLAHFRRAPRAFFKAFEESEATRAELRRRFADSSAVT
                     ERAARAAAGRFDLLGLKDLSFGKPVDWNFEPVAGKHSPMTHWSRFAELDAEQAGDKKI
                     IWELNRHQHFLTLGRAYWHTGDEAHARAFAAHLASWMEQHPPKAGVNWMSSLEVAFRS
                     ISWLWALHFFKDSPHLAPDTYARALKYLYLNGRHLETYLSTYYSPNTHLTGEALGLYY
                     LGAALPELRPAARWRAAGRRILLEQLTRQVRPDGVYFEQASYYQRYTADFYTHFLLLA
                     RAAGEPSDAEVAPALSALCDHLMHLTAPDGTTPFYGDDDGGRLAPLDERPPADFRATL
                     ATAAAVHARPDYKYVAGPASEETLWLAGLDGLQVFDALDARPPAEASRAFRDGGYYVM
                     RDGWARESSYMLLDCGPHGAMNCGHAHADALSFTLAARGRTLLVDSGTCTYTGSGEWR
                     DHFRHTAAHNALVVDGESSSVAAGAFSWSAVARCEAREWLSRPRFDFFEGAHDGYRRL
                     PDPVTHRRSVLFIKGDYFIIRDEADARARHRYGLRFHLAPDAPSQVESGRSAVRLLDS
                     SAPGVELHAHGGGGRWHAEEDWVSTCYGSRSAAPVWTFEAEGEGPQDFLTVVLPRAAG
                     AEAARVAELTHDGGAAHSLEVALGGARDLLVIGRAGHTVSAGQLVTDFAWTWARLAAD
                     GSLAEAVLVGGGTRFHFGGRELLASPRRLRCAVARTVEGRLEFETESEGGESTDGVTP
                     PDLVSVGGAPKGRDNLSKEGRGPAARAVETPHPRRGL"
     gene            complement(29999..31012)
                     /locus_tag="VF240_14940"
     CDS             complement(29999..31012)
                     /locus_tag="VF240_14940"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7176560.1"
                     /translation="MKHSALKLLTRAGAFAPFRAANRGRVLILTYHRFGRGGDPLATP
                     EAEFDAHLRYLKQHYTLVPLSRVAAHYAGAGELPARAACVTIDDGFADAYEVAFPLLQ
                     RHGVPATLFVITDFLDGRAWMWTDKLRHVALAAEGARLEAEVGGRTFDLRLAGRDTRI
                     SAAGRVNSELKRLAPRERESAIERIAAAHGVALPALPPAEFGPVTWAQARELDAAGVE
                     IGSHTVSHPILTTTDDDELRRELRDSRARLEAELGREVELFCYPNGVFDERVRRETAS
                     AGYLCAVTTEPGLNERRGADPLALRRVPAETDLPHFVQSTSGFEQLKNRLRRREPAAV
                     DVY"
     gene            complement(31009..31872)
                     /locus_tag="VF240_14945"
     CDS             complement(31009..31872)
                     /locus_tag="VF240_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HEX7176561.1"
                     /translation="MTEKGSDRKFRVILLTHGGAELVLERLLGVEGVEVAGVFVETET
                     TPRRDWREKLRRSIRYDGWAATAAKLAGQLLRRRAGTDVMEVGRDALRETCTARGVPF
                     HLVANYHTEEAAELLRAAQADLGVIYGTNIIRESVFRLPRLGSINLHQGLAPFYRGGP
                     PVFWELYNGEREVGLTVHFVAAKVDTGEILLQEAAPLAYDDAHGDDFEAFIEEYTARL
                     RARSAELVARAVGMIAAGTYEARPQDTSLGKRYRLPVKREKDELRRRLRARRRQASAA
                     AKGHAADAEGR"
     gene            complement(31930..33108)
                     /locus_tag="VF240_14950"
     CDS             complement(31930..33108)
                     /locus_tag="VF240_14950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7176562.1"
                     /translation="MPRNVLQLMGSFHQGGSERQAAQLTRLLAESGRYRVRVACLNPE
                     GVLRGEVERLNLGEIDAYPLNSFYDRRFVAQLRRFAAFLRAESVDLVQTHDFYSNVFG
                     MAGAALAGVGARVAAKRETGGMRTAAQRRVELIAYRLARAVVANSEAVKEQLVRDGVP
                     ARKVAVVYNGLDPARVRPPADFSRAEALAGLGLPADVGRRFVTLVANLRHAVKDHPTF
                     LRAARRVRGEFPSAAFVLAGEGELETPLRALAAELGIADAVFFTGRCTQVGALLAASD
                     VCVLSSTAEGFSNSILEYMAAGRPVVATDVGGAREAVAEGETGHLVPAGDDERMAARV
                     GGLLRDPVRAREMGERGRERVARQFSCDAQLARTEALYEQLLERNRTASGSERVRVAG
                     "
     gene            complement(33114..35036)
                     /gene="asnB"
                     /locus_tag="VF240_14955"
     CDS             complement(33114..35036)
                     /gene="asnB"
                     /locus_tag="VF240_14955"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7176563.1"
                     /translation="MCGIAGFIEPTSTGVEEGPSRPALIDRMCRVMAHRGPDEQGAEV
                     LGRAAVGMRRLSIIDLAGGHQPMRGCDERVRIVFNGEIYNYKELQRDLEARGHKFRTR
                     SDTEAIVHAYEEFGDACVERLRGMFAFAVWDGRNERLFAARDRAGEKPLYYTTTPGGS
                     FVFGSELKCLLEHPGVARETDPAALDAYLTLGYVPEPLSVFRGVRKLEAGHRLVLEKG
                     RVTTESYWDFPVETDDPPRSEEEYVEELRARLDESVRMMLVADVPLGAFLSGGVDSST
                     IVGLMSRATNAPVKTFSIGFREDSFDELKYARLAARHFETDHRELIVTPEVCQLVDEL
                     VRHFDEPFADQSSVPTYLVSKLAREHVKVVLSGDGGDELFAGYTRYAVEQGRQGFARL
                     PRLMRRGLMAPLAGRLPHGARGRNFLHNVALDPAERYLDNVSTFNELGKGALYTADFR
                     RALGGREPAAEIFRGHAARASAGGGVASLLYLDAKTYLPGDILTKVDRMSMAASLEAR
                     VPLLEHKLIEFVSRIPASLKMRGTESKYVFKRAVRGLVPDEILDRPKQGFGVPIGEWI
                     NRELRERIRDTFADARTRTRGIAEAAYVDVLLAEHERGRRDHSNALWSLFILELWHRA
                     YADAAPVGEELEAIAT"
     gene            complement(35127..36329)
                     /locus_tag="VF240_14960"
     CDS             complement(35127..36329)
                     /locus_tag="VF240_14960"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7176564.1"
                     /translation="MTTVAEILLWASVFALFYTYAGYPLLVWVVSRVRARPWTRAAYE
                     PSVTVVITAYNEERDLAAKLENTLALDYDPAKLEVLVASDCSTDGTDEIVRSFARRGV
                     RLHRQPERLGKTAAQNAAVELAGGEVILFSDATTLYRRDVLRAITPNFADASVGCVAG
                     HLVYVDTANSSTGQGARSYWGYETFLRRHESRACSLVGAPGCLYAVRRAAYVPLYHEA
                     CSDFIIATKMVEQGLRAVYEPAAICTEETNSRSGKELNMRVRIIAQTLADLWRHRAML
                     NPLRSGFYAVQLISHKLLRYLVPGFLLTCLAASLALAPGSGFYTAAAIAQVLCYGAAV
                     AGLLLERAGLHSRLLALPQYFLLANVAAAVAFFKFARGERYARWEPIREGGAGGQSAQ
                     LRDRQGDA"
     gene            complement(36326..>37349)
                     /locus_tag="VF240_14965"
     CDS             complement(36326..>37349)
                     /locus_tag="VF240_14965"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7176565.1"
                     /translation="ISPGEAWDNFVEFLKVISIFVVIVNVVRTERRFRGMLILAMAVS
                     VLVSFNAFDDYRSGLLEMRGERVKGVLGGLFANPNDLALHLVSMVPLAAGLALARRGA
                     LKKICYGVCAALFVAGIVVSFSRGGFLGLVAVTGVVAWKLGRKNRVVVAALMAVTFAV
                     FVAVAPGEYGSRITSMFGGDITGSVDARRDLLWRSVLVALRYPIFGVGLGNFHHRGAQ
                     EQVSHNAYTQVAAEMGIAALVVYVMFMVTSIRGLRRIERESFSEKDRAGFYYLSIGLQ
                     ASLIGYMVSSFFASVAHLWYVYYLVGYAVCLRRLYVLKYGEAGSEPSGAKLDHPQIES
                     ARIPST"
BASE COUNT         5989 a        12380 c        12992 g         5988 t
ORIGIN      
        1 gatcacgtcc tcggggccga gccgatactg cgtgaggaag ttgttgtaga agggaagcac
       61 ggcggcctcc tcctcgctcc gctgcccgat gcggttggcg agcatctctt tggaggcgac
      121 ggggcgggcg accgtggcgt cgtccggctc cagccgctcg cccggcgtcg gcgcgggatg
      181 tgccgccttc acctcgccct gcggcgattg cttcggcggc tccttcgact ccttctgcgc
      241 cgcggccgtg gcggccgtcg gcgaaagcgc gagcaggagc gcgagtgatg cgacgccgat
      301 tctcatttcc gatttcatcg tcttaacctc caaaacttcc gttaacaact ccgaagagtc
      361 cgagccttat gtgccccgcc cgaagaggat cgtcttcacc gtctcgaaga ggatcacggc
      421 gtcgagcatg aggctctggt tcttgatgta gaaaagatcg tactgaagct tctgccgcgc
      481 gtcctcgatg gaggcgccgt aggggtactt gatctgcgcc cagccggtga ggcccggcgc
      541 gatcaggtgg cgctgctcgt agaagggaat ctcctcggcg agctggctga cgaagtgcgg
      601 ccgctcgggg cgcgggccga cgaagttcat ctcgcccttg aggatgttcc agaactgcgg
      661 aatctcgtcc acgcgtatct tgcgaatcac ccggccgacg cgcgtcgtgc gttcgtcgcc
      721 ggccttgctc gcccacacgg ggccgtctct ttcggcgtcc aggcgcatcg agcgaaactt
      781 catgatcgta aacacccgcc cgttcttgcc gacgcgctcc tgccggtaaa gcgcggggcc
      841 gggcgactcc agcttgacga gcagggccgt gaggagcgcc agcggaagcg acagcagccc
      901 gccgaccagg gccgcgccgc gatgcaggaa cgtgcgtgcc gcactgctga ggcgcgcctg
      961 ccggccccgg cccgagaaga tgagccagga ggggcgcagc atgtcgagcg agacgcgccc
     1021 cgtcagccgc tcgtagaagc tggcgcactc ctcgatggcg acgccgcccg acagactcag
     1081 gtcgagcagt tcctcgacgg ggaactgtcc gcgccgctcg cccatcgcga cgacgatgcg
     1141 gtcaaccgac tcgcgccgca cgacctcggc taagtcttta gtcagtccga tgacgcgcgg
     1201 gttcaccaag ctctggccga ggagttcggg ccggttgtcc acgaagccga cgatgcggaa
     1261 gcccgcgtcg cgtcgttcca aagcctcgcg cgccacctcg atggccgact cgcccgagcc
     1321 gacgatcagg atgcgctcgc cgacgttggg gtggccgagc agccagtgaa tcgccacgcg
     1381 ccagccgacc atcagcgtga gcgccagtgg cagcgagatg agcgacacgc cgcgaccaac
     1441 catcagcggg ggcacggcgt agaagaggat ggcgagtgcg acccacgcga gaccgagcgc
     1501 ctggatgaga cgcaacacca gctcgcgccg gtcgtgcatg acgacgaagt cgtagaggtc
     1561 gtagagatag aaagcggcga ggcagaagag gccgacgagc gcggccttca ggtagccgtg
     1621 cttgacggtg agttcaaagt gcgcgtcctc catcccgagg cgcacgtagg cggcggccac
     1681 cgtggcgccg aagagcaaaa gcgcctcggc ggcgataagc agcggcgtcc tcgtgttgaa
     1741 gcgcgtcgaa ctcacggact ctcaatgacc tctcaacaaa agctgttagc tgttagcttg
     1801 aagatagcga accagagtgt tcgagtgcct gcgaggcgtt cccttcggcg tctgccgtgg
     1861 gctgaaagct aatagctcac agctaatcgc taatcgctct ctcacgcctt ctcctcgacg
     1921 acctcggccg tttccgccgg ggcctcgtcg cgccgctggt agcgcgggcg gcggtagtag
     1981 tagttgttgt tgtagttctc gcccggcggc tcgtccgcgc cgttgaggac gatgccgagg
     2041 acgcgctcgc gcgggatggg ttcgagcagt cgttcgagcg cggagaagcg cgtgcgcccc
     2101 gcccgggcga cgacgagcac gccgtcggcc cggctgatga cgagcgtggc gtcggtgaag
     2161 atgccgagcg gaggggtgtc gatgatgatg tagtcgtaca gcccgcgcag ctccttgagg
     2221 acgcggccga agcgcggccc cgacaaaagc tcggcgacgt tgtcgcgcgg ctcgccgccc
     2281 ggcagcaggt gcagccccgc cggagtcagg cggatgatcg actcttcgag cgtggcctcg
     2341 ccggccagga cttcggagag gccgaagggg gcgtcgatgc caaggtagtc gccggccgtc
     2401 ggcttgcgga ggtcggcgtc gatgagtagc gcgcgcacgc cgtcggtctg cgcgagcagc
     2461 caggagaggt tgacggccgt catcgtcttg ccctccatga tgccggcgct cgtgatgacg
     2521 aaggcctgca tcttcttgcg ctcgccggcg tggagcacgc gcgtccgcaa agagcggaac
     2581 cgctcggcgt gcggcgagtt cggttgggtg acggcgatga tgtgtggttc gacgcgcgca
     2641 gacgtgacct ccagagactg gaaggcgtcg gcgcgcatcg aaccggcagc gcccaaagtt
     2701 gccccagcaa cacgcgaagc ctgcatgtcg gggagtgccg acccgacggg gcgtccggtg
     2761 cgctcattgg gcgctgatcc tgcgggcgcc gaccccggcg ccgtgatgtg tgcggagcgc
     2821 ccgagcaatt gctccggcgc ggggagcggc gcgacaacgc ttgacgccga cgcgtgcgcg
     2881 gcgtcgccgc ccgctccgtg gccgttcgcg ccgttgcccg cggcgcgctc cgtcgccccg
     2941 ccgcgcgact gctcgtcgcc cccacttccc tgacccttcg cgccgttcgt ctccgcggcg
     3001 cgctttaatg cgtcgtagac tcgacccatc tcacttaccc ccttcgcggc cccagccgtc
     3061 ggacgcgcgg ccgaggtcgc cgccactgtc cgccaccgct gtggcgtccg agccaagttc
     3121 ccgctctaac tcgcgctcca tctcgcgctc ctcgtgagcg tgcgcggccc cggccatcgg
     3181 gccgcgcccc tcgtctgtcc cggcgcccgc cgcccaaagc tccgcgcgcc cctgcgcggt
     3241 aaagatgcgc gactgcgact cgcgctcctc gcccgtctgc cgtccgcgcg aggccgtggg
     3301 cagcaggtcg aaggtgtcgg ccacctcctc gatgatcgcg cggttgatgg tttcggcccc
     3361 tgcggcgaag cccgagagca gcgcgttgtc gcagaggttg ttgacgagac gcgggatgcc
     3421 gttcgagcag cggaagacga aatcgatggc cgtctccgtg aagatatccg tgcgctccgc
     3481 gcccgcgacc agcagacgcg acgagatgta gcgttccgtc tcctccacgt caggtagcgg
     3541 cctgatctcg caccgaagcg ccacgcgctg cttgagctgg cgcagctcct gacggttcaa
     3601 gacctcccgc agctcgggct ggcccgtcag gactatctga agctgcttgg ccgtgtcgga
     3661 ctcgaagttg gagagcagtc gtatctcttc gagcacggcc gtggagagtc catgcgcctc
     3721 gtcaacgatg agcacggtgc gcaggccgcg cgcgtgccgc tcttcgagcg tgtcgccaag
     3781 agcgatgatc agctccgact tgttgtgcca cgactgcacg ttgagcaggc gggacgaggc
     3841 gatgtgctgg tagaactctt cgacgctcat ccgcgggttg aagatgtagc cgacgagcac
     3901 cgtgcggtcg agccgctgca tcatccagcg caggatggtg gtcttacccg tgcccacctc
     3961 gccggtgacg acgattaagc ccttcccact ctcgatgccg tagtgcaaat tcgccatcgc
     4021 ctccgtgtgc gacggcgtga aatagatgaa gcgcgggtcg ggcgtcagcg cgaacggcag
     4081 ttctttgaga ccatagaagt ctaaatacat ctcaacctcc ggttgaagag gtcagaggtc
     4141 agaggtcagt taaaaggtca tctgttttta ctaacctcta acctctaacc tctgacctct
     4201 ctttcctcat cctctgaacg cgaacacctc aagcaggcgt gtcagccgca gggcggcgta
     4261 gagcgcgggg acgctgagga cggccgaggc caacccggcg gtcgtgagcg ccacgcggcg
     4321 caggcgcaac cggcgctcct cgcgcggcgt gagcatttgc gggagcgtca ccagcacggg
     4381 caggcccgta tagtgctcgg cgtcctcgcg gttctggatc gttaacagct tcggcacctc
     4441 gaaggcggcg gcgaagacta gccccgtgca gaggcccgcg acgaggccga ggaacatcag
     4501 cagcgggcgc ttcggcgcca cgggctgctg cggcaaactc gccgcgtcga tgacctcgat
     4561 tgactcgccc tgcgagttcg acgtgacatc cgagatgagc ttcgccttct cgctctgctc
     4621 cagcaactcg tcgtagacgg tcttggccgt ctggtagtcg cggttgatgg attcgagccc
     4681 gacctccgcg gtgggcatgc cgccgaggcg ccggttgacc tcggcggtct gctggtcgtt
     4741 gagggcgagc cgcttctgca acacggtcat gtcgccgtcc accttctgca actcctcctt
     4801 catgctgtgc tggcgcgggt cgctcgcgcc ttcgagccgc ttgcgccggt cctcgatccg
     4861 ctgcttgtag tcggcgacca tctcgtcgat ctcgcgctgg acgttgtcga gctcggtctg
     4921 cttcatgacc acgtccgggt gcttcggccg cagctccgtg agcatccggt ccttgtcgga
     4981 gcggagctgc tcgcggcggg cgacgagcga ggcgtaggcg ggcgtcgtct tcgggtcctg
     5041 gatgttgtcg atgaagaggt cgatctcctc ggcccgacgc ttctcgaact cgccgccctg
     5101 cgagacgagc gaggagcggc gctggcgcag gaagtcgagc gacgtgttga ggctcttctg
     5161 ctcctcgcgc aggccggcga gctggccgac gagcgcctgc gagtgcgagg gcaggtgcgt
     5221 cacgttctgc atcatgaact ggaggcggcg gcggtcgatc tcgtcgagtt gctgcttgga
     5281 ctcctggagc cggcgctcga agaagtcgcg ggtcgccgtc gcgtcctgcg tcgaggccac
     5341 cgtctgcgcc ttcgtgtatt tcgacgccag ctcggccgcc acggcctgag cggcctttgg
     5401 gtcggagctg cggaaggaaa gatagaagcc gttggtgatg tcgttgcggg tggtgttgat
     5461 cttgacggcg aggtcgcgcg tcttcatgag ctcgacgagc acgtccatcg gctcgtcgcg
     5521 ctgccgctcg gcggcgtaga ggttgaactt caggatgagc ggctccagtg aagagcggct
     5581 gacgacctcc tggccgatgc tgttgatgcg gatggtgagg tcgtcgcccg tgagctgcgg
     5641 gacgatggac ggcgagatcg acggcggacg caccgtcaac agcgtcgtcg attcgtaaat
     5701 gttcggcagc ttccacaccg cccacccgac cgccagcgtg atggccgccg ccggcagcat
     5761 gatcatccac ttgcgccgcc aaagcatgcg cgcgtactcg gccggcttcc gctgtcttaa
     5821 ttcaacactc ataagctcac tccgttcgcc gtgataagtg acgagacaaa aaccgtctgc
     5881 tctttcttat cactcatcac ttatcactca tcactcctca aaggctctcc agcgcccgct
     5941 gaatgtcctc gaccggtgct ttgggccggc gcagcggccc gacggcctcg gcctgagcga
     6001 gacgctgggc ggccagcaac tcgcggcgcg cggcggcgcg gtcgcccttg cccgtgaggg
     6061 cgaggccgag gtggaggcgg tagacggagt tgtcgccgcc ggtcgccgcc gagagcgaga
     6121 cggccttctg aagttgttcg acggccggcc cgtagaggcc cttcttgtag tagacccagc
     6181 cgagcgtgtc ggcgtagccg ggctgctcgg ggcggcggcg gacgacctcc tgcgcgagac
     6241 gcacggcctc gtccaagttg cccttctcgt attcggcgta gagcatcgcg aggttgttgg
     6301 ccgcgatctc ctcgccgggg ttgatggcga gggcgcggcg gtagttctcg gccgcggcgt
     6361 cgaggttgcg gcggctgaac tcgaccatgc cgatcttgct gaaggtgacg gcgtcgttgg
     6421 gccgccgctc cgtcaccttc tggtactcgg ccacggagcg ctcaggctgg ttcgtgttga
     6481 agtaaagctc ggccagcttc tggtaggcgg ccacgaagtc ggggtcgagt tcgatggcgc
     6541 gccggaagcc ggcctcggtc gcctccgcgt cgggcggcac gccctgcgtg gtgccctgcg
     6601 cgtaggccgt gcccttgagg tattggaggt gggccacacc ggggtgctcg gcggtgaggg
     6661 cgtcgaggcg cgcgcgcgct tcctggaagc ggttcgtcgc gccgtagagc tggacgaggc
     6721 cgtcgagcgc ctggaagttg gtgccgtcga gggtaagcgc gcgctcgtag agagagaacg
     6781 cctcgtcgta cttgccttcg acgacggcga tgccggccag gttggtgtag gacgaggggg
     6841 agttaggcca cacgtcgcgc gcggcctcca tgtcggcgcg cgcggcggcg aggagcgagg
     6901 ctgccgcgcc cgcgtttttg gccgcgccgc gcaggcgcat gttggccatg ccgcgcgccg
     6961 tcagcgtgcg gactttaatc tcggagagca gttgcggcgt ctgctgcgcc gagggcggcg
     7021 tctcgcgcag cctgtcgagg agttcggtgg cctgccggcg aaccatctcg gggtcgccgc
     7081 ccgagaggtt aacctgcaac tgcatcagct tcgcgggcag gaagttcggg tggaagcgtt
     7141 cgaggtcgcc ggtgaacgag cgcgcctgct cgatctgccc gtcgcggaac tgcgcctcgg
     7201 acatgaagta gagggcgagc tgcgagcgcg gctcctgttc gagtatcccg ctgaggtcgg
     7261 cgatggcctc tttggtctgg ccctgttcga ggcggaggcg cgcacgcagc agcagcgcct
     7321 cggggtcgcg gccgttcttc ttcaaaatct cctcggcctt cgccgcggcg ccggggcggt
     7381 cgcccttctg gaggaggagt tcgccgacgc ggtagtgggc gcgcgcgtac tcggggaaga
     7441 gttggaggct ctcgcgatag accgagagcg cgtcgtcgag gcggcccgtg ctggcgtaga
     7501 agtcggcgag cttggtgcgg ccgtcggggc gcgtggggtc gagcgcggcc agagccttgt
     7561 aggcctcctc ggcgcggtcg agccgattgt tgaggaggta gaagctggcg agaatccagt
     7621 gtacgtcctg attcgtcggg tcaacttcga cggccttgcg gaactgcgcc tcggcctcgt
     7681 cggtgcgccg ctgctgcgcg aggaacttgc cgtactcgac gtaggccagc gaggcgcggt
     7741 cgttcaactg gatggcgcgc ttaaaggtct tctcggcctc gcctaagtca ccgacaagac
     7801 ggtagaagcc ggcgaggctg atgtaggact cgacacgcgc gggatcgagt tcgatggcgt
     7861 gattgatctt cttgagcgcc tcgacgcggt cgttggggcc gttgcgcgcg gccaggacgt
     7921 tggccagcag aatgtgcgcg ggaacgtagt tggcgtcctg cttgaggatt tcttcggcgt
     7981 ggatgccggc ctcttcgagg agttcggggt tggtgacctt gctgccgcgc tggctgccga
     8041 tgaggaggta gctgccgatc ttatagcgcg cctccttgtt cgacgggtcg agcttgaggg
     8101 cgcggttcat ctcgtcgaac gactcgttga accgttccag cccctcgtag gcgcgggcca
     8161 gcccccagtg ggccgaggcc aacgcctcgt cgatctggat ggcgttacga aactccagcg
     8221 ccgcctcgtg gaacttgcgc tccttgagga aggcttcgcc gcgggtgaca tgttcggctt
     8281 tgagcttttc ggggtcggag caggcggccg tcaggacggc gcaggcgagc aggacggcga
     8341 gcagcgccgg ggcgaagccc cgcgcgcgta ggcactcgaa gaatctcatt cgtggcactc
     8401 ctatcggaat cggcgtcggg ccgagcacgc tgtcgggccc ggggcagggc ctttcctcgt
     8461 ctttagcctg acgctcgtct taacttcaca aaactcccgc agcgggcaag gcaacgaagg
     8521 tttgtgctcg gtctccctca ggcgttagcc cggaaaggtg acgaactact ataaaagacc
     8581 aatccggccg ggtttccaat aaccggcccg gcgtgtttca acccctagtt cgggacaaag
     8641 cgcggcagct cgggcgagac gcgcgcggcc agctccgccg ccagattcat cgcctcctcc
     8701 tccgagcgtc gcatggggac gatgacgcgc accatcgcgc cgtccgagcg ccgggagcgc
     8761 atgctgtccc aggccgtgta gagcttgtcc cagtattcgc tggccacggc gcggccacgg
     8821 ccctgatacc agtagacgag cgcgaagcgc tcgctcgcgt tctggataac gtagcggttg
     8881 gcctcgaagg ccgcggagcc gtccgccggc tggacgcgga tgacggccgg gtcgcttaga
     8941 atccagcccg aaccgggcat gcagttgagc gggctgtgat aggtagcgcc ggcgcgctgc
     9001 gaggcgtagt agccgacgta gagcgaagcc atgcgcccgt cggcgagcgc gtagtcgcgc
     9061 agcacgtagt cgtcggcctt gagcacagcc tcggtcgcct gatcgaaacg cacgtcggcc
     9121 cgtcgctgac tccactcgcc gagcgaggcg ggcaactctg cgagcgcgct gcgcgggacg
     9181 cgcgcctcgc cggcgcgctc ccagaactgc acgaccgccc cgcccgcgac gagcagcacg
     9241 acgagcaccc agaaatgtct gtcccgagtc ttcatctcac aaatccttca gacggcttcg
     9301 cccccgcccg tcatcgccgc cgacggtgcg ccgaccgtga cgcccttccc gtcgctgccc
     9361 gtgtcggcct tcgcgccctc ttcagtcgcc gccgcgctac tgcctccgcc gcgagggcgg
     9421 ccgcggccgg ggtcgaagcg gtcgatgagc cagccgaagg cgaagagcag caggaaggcc
     9481 accacgtaga cgacccagcc ggagaaggag tggaagaagc cgtcggccac ttccgtcccg
     9541 tagtagcgcg cgagcacgcc cgtgccgctg acgcgcccgg cgttcgtcag gatagcgatg
     9601 ggcacggcgg ccagcacgat taggacggca cgcagcactt tgaagttgcg cgcataccaa
     9661 ctgcgactgt tgccgccgtc gtcgccgccg tcggacttcg gcgaagtgaa gtaagcgtag
     9721 acgaccgcga gcgtcactag cgtcatcagg ctgcggatgc cgctgcaagc ttcgacgact
     9781 tcgagcttct tcgtcgtcat cgaattcaac gggagaaact cgataatgtt gccttcgcgc
     9841 aggaccggga tgtcgaaagc gcgcatcgcc cagacggcgc accgcgaggc gaagagctgg
     9901 agcgggaagg cgaccttgtt gaagacgatg gtcggaatcg ggattgccag cgccagcagc
     9961 agcagcggca cgacgaccac gcgcagaatg cgcccgcccc agaagtagac ggccacggac
    10021 gcaagcaaga gcacgagtga catccgctgc acgaacagtt cggcgccggc cacgccggcc
    10081 cacagcgccg cgagcgcgca cgccgcgccc gccaacccca gcgcgggcca ggggcgcccg
    10141 gcgtcgcggg ccagacgagt gcgctccgac cagaggatgt agccgatgat gaaggggacg
    10201 agcaggccgt gcgagtagtt ctcgtccgtc caccagaagt ggccgagttt ggcgagcaca
    10261 ccccagtagg cgaaggcgat ggcgccggcg atgaggagtg gcctgaaaga aatccgctcg
    10321 gtcgtctgca ttgtgtttga ggattggcgc aaaatgtgga cagcagatca cacagcccgt
    10381 ggcccagtgg tttcgcgaag acggctgctg gccgcccccg tcgcggggag acgcgagcca
    10441 agagcttagc gcaactgggg gggggacggc aacatgagtc cgacctcgag gagtggcgcg
    10501 ggagggtcgg cggccccacc ctttgcgtat ccggcaaacc ccgcaggcgc agactggcac
    10561 ttcaaacgga gtgaggcgcg ggggttttcg gcctctccgc cgcctcccgc gcctcgcttc
    10621 gtgcgcggcc caccctgagg gccgcgaagt ccttaccgcc tcctcgaggg aggttcatta
    10681 ctactgctgg ccgaagcgcc ctctgtactc gcccgagacg ataaaggatt tgaccatctc
    10741 ggccgcgacg aagtcgccgc cgtggttctg gagcttgacg agccagaagt tgtagcccac
    10801 cgggtccggg tcgcggcgca ggtaacccat atactccatc atgacgaagc ccgggatggt
    10861 ttcgctctgc acgaactcct tgcgctcgac cacctgccgc agcacggagg cgcgctccgt
    10921 gcccgcgtcc aagagcgcca cccacgagtg gtgctcctcg gggctcaacg tcacgcctgc
    10981 gttgtttatg atcgcgtcta cgtactgcgc gttcgtcagg ctgtcgtact tcgcgccgaa
    11041 gtcgcgccgc cggacccact cccggtagaa ggccgccata ttagcctcca acttctgtct
    11101 ccacccgggc gcgccgacga taaccccctg accgatctcc gcagcgccgg cctgaagttc
    11161 cgacatcgac ggcatccggc cgtaggacac cttgttgagc cggtggatga ggaagccggt
    11221 ctcctggaac tctatcgaga ggaagaacgc cgccgagacg tgaacgcgct tgacctcgac
    11281 gcactgcgcg tccgacccgc aacttgcaat ctcgttggtc cagaaggcca gcccgctgtc
    11341 atccggctcg cggttaagga aatccacgta atgctgccgg acgaagaact ctgtcacggc
    11401 cgcggggttg ggcacgtccc cgtgggccat gtacacgttg gcgaattcca ggtggtcgat
    11461 ggccacccgc agggcgccct tcgcatcctt aaggtttccg tcgcgggcga gacctgtagc
    11521 gagcctcgcg tagttcaagg cgttctgtag ctcgccgtcg atctttccgg ccgccgcgta
    11581 agagccgctc tcctcgacga agtcaccgta agcctgctga atgtcggaag ccacggcgtc
    11641 tagtttgggc acgactgagc tttgcgccgt cgagatgccg ccgccctcca cactgctgct
    11701 gctgctcccg gtcaaatcgg agagctggtt gctagtcgtc tgggcgttct tcttcgcccg
    11761 acccacggac cccgtggacc tcttaatctg caccgtaacc ccgttggaga cgacgcttct
    11821 gcccttgcta tccgtcgcct tggcggtcag ggtgtaattg cccgccgcga cgttattcca
    11881 cgcgcagccg taaggggcgg tggtgtccgt gccgatcagt gtggctcccg agtaaaactc
    11941 caccttggtg acaacggcgt ccgtattggt ggccgtcgca gtgaccgtaa ggttggcgac
    12001 ggcgttgaac tgcgagccgc tgagcgggct cgtgacggtc gccatcaaag cgcccgcgtc
    12061 aggcgtgggc gtgggcgtcg gagtaggcgt gggcgtgggc gttggtgccg gcgtcggact
    12121 cggtgtcggc gatggcgccg gcgccgcgcc gggcggcgtt atctttgcca catacaggtc
    12181 cgttcgcgtc ccgcccatgt tcgacgtgta gaacacgaac cgaccgtcgt acgagatgtt
    12241 cgcacggggg ctgtcctcgt atttgcggta gacgctacgg tggtgcagga gcctccgcac
    12301 taggcccgag ccgtccgtgg cgacctgaat gagttccttg cggagcgggg cgggggcggg
    12361 agccggggct tcgccgaaga ggccgacgag cgcccagccg ccgtcgtccg cgagcatcga
    12421 cgtgtggatg gcctgtgtcc aatcgggcag tgtcagcagg ggggcggcgg cgtgcgggga
    12481 actgaggcgc cgccagttca tccggttggt gttgttgtcg tacccgacgt atgtatccgt
    12541 gccggcgtcc ccgtggccgg gcgccccatc ccggtcgtcg atgatggggt cggagacccc
    12601 ggtctccacg ttgtagacga gctgctcgac cgtgccaggc ccctgcttat ttgtcttcac
    12661 cacgacccac cgcccgtcgc cgctaagtgt cgtctcgtcc atgtcggtgc gcagcacgct
    12721 taacatcagc ctgcccaacg cgcggtcgta gacgccgaag cccacgggcg cataatcgga
    12781 tgagcgtttg cgggtgaagc tgacccggtt gccgtcccga gagaacgagg tctgaatcat
    12841 gtactcaccc gggagctgcg ccgtaaggtc ggccaggagg tcataccgtc tcgtgtcggg
    12901 gtggtaggcg aggatggccg cgcccacggt gcagtagagc gtgttggggt ccgtgcggct
    12961 ccacaccgcg ccctcccaga agggcgtgcc gcgcgtcggc tccatgggca actcttccct
    13021 cacgccgcgc gcgaagttca cggggtcgaa gcgcagcagg aaggccgggc tgccgtagtt
    13081 gccgacgagg acgcgcgtgt tgtcggagtt agacgtgggc cagtaggagt agtaagtgcc
    13141 gcagcccgcc gggcagttgg ccccgtccgt gaggcgcatg acctccacgc ccgacagcga
    13201 gtcgatgaac ttgcccccga cgggcggagg cgtgagcgtc ggaccttccg cgtagacgcc
    13261 catgtccgtt ctgacttgcg ccgtcgccga caggcagagg atgaacaggg cggcggccgt
    13321 cagggcgagg gaaaaagagc gacggctggc gatagccagt ctcagattgg tcacggctgg
    13381 gactccttcg gtaaccggac gatcttggcg atttgctgcc gagactcctg gctttgcgaa
    13441 cccgcatcac tgcgggggtg cctttatcgg taatatcagc gctaaacggt taacgcagac
    13501 gagaagctgt gatctgactg gtgagtgggc gtaggagcgt aacggtgacc gggaggcgtg
    13561 cggttgcacg cctccggcca cgattcgctg gctgaaaagg tagcttcgag ttatcggtag
    13621 aaggggtatg aggtgcttcg gagaattcag gaccgacgat ggcaagatag gctgcgcccg
    13681 cgcgtggttc cggttacgga gtcaaagtct ccatcgccgc acgccgaacg gacattgtgt
    13741 gaggtccggg ctgggcgccc gaccagacga ctggcgtgct gagcaaggga tattgagccc
    13801 ttaactgtct ccgatcgcct tcggcagcca gacaatctca cacttcgcga cttcaaagat
    13861 aaagagcatt gaagcgttgg gagattcacg gctttgtgcc cccgttttac agcgggttta
    13921 ccttttcgtg tgcaatcgtc agattcacaa agaacattgc cgcaatgatg cagcagtcac
    13981 aataattaaa cattttgaca actatgttgt caattgtttt ctttgataga atttgattaa
    14041 tatcagtgcc accttagaat tttgataatg gaggagctat tagttgtatt ataacgcaga
    14101 gaagtgagaa gtccggtaga caattccggt cgtctggcaa gtgcgaaaac agaaccccaa
    14161 atataagcct gaataaaaaa aaatatgaac aagctctcca aagtcatctc gtgtatcacg
    14221 ctggttttcc tactcctggc agcctccgaa gcgcgggccg acaccgtaga aataactagc
    14281 ggattcgtct ctatcggcgg cgtgtaccca ccaggccggg gtacattccg caccataaat
    14341 ttttcactcg cgggagataa ctttacgttt aacggtactg aggtcgacgg gactactcag
    14401 caggtgacga cttgcacctt cggcccctgc cccgccggta ctttaatcaa ctcaagcagc
    14461 gttgtgagac ttcagggtgt cggctcggcc acgatcaacg gcctgttcta cccaattacc
    14521 cagccgcttg gcagcctgct aacattcacc ggggaaagca tctctattcc atccagcggc
    14581 gaacccgtca tcttcatcac tacacctttc acgatgacgg gcacgctcaa cgttgtcgtc
    14641 ccgccgggaa cgtccgtttt cagcgcgacc gctgtgggtt ccgggtttgc gacacttacc
    14701 ttgcgacaat ttgagtccgg ctacgtcctc accacaatcc gctacgactt ccagaggccg
    14761 atacctgaac cgagcaccat aatattactc tgtacgggtc tagcgggtgt ggtaagagtt
    14821 acccgccgcc gccggtctaa gatttagggt ggaaaaactc ggcaactgcg ggcgaccagg
    14881 ctgacatgga cgctgcggcc cgcagaacac caatctccgc ttttgatgac aaccgagtcg
    14941 ctgcaagccc tgcccctccg cacttcaatc ttgtcagatt attatcctca acagagatga
    15001 tcttccggca tagctgaggg acgcccgatc tggaataggc gatcagcttg tgacttaacg
    15061 cggagcatgt ctggagatta agaacgaagg ggtcagggga ttcgagtcgc gcagttcgcg
    15121 ctgaatttcc aggccgcggc gcgctagttc gcgccgcagg agggtgacag tctgaaacgt
    15181 ctcccgactc ggtaggcgca tgcgcggctg ccgccccgtg agctggtaga taagcccggc
    15241 cgccaggacg cgcacagcgt gcaccgccga gggcaattcg ccagcacgta gcgagtcgcg
    15301 tagtttgcgt gtgtaatagc gagcatggtg aattttaagc aggagtgccc cgccggggcg
    15361 cagtacgcgc gccaactcgg agaggcaacg cgcgttatcc gtatacggga gtgcgaggcg
    15421 acagatgact acgtcgaaag agccttcgtc aaagggcagt aactccgctg cggcacgaac
    15481 gaaggtcaca ggggcggagg gcgcgttggc cgcgaacaac ctcctcccta cgagcccaac
    15541 ttccggcgtc acgtcaactc cgacacacga cgcgcccagc tcggtagcga acggcaggag
    15601 ctcctgccct gccccgcagc ctaggtcaag gacacgcccc acgtcgagcc cggccacggc
    15661 ctccagagac tcgcgagcgc gcgacgcatc gccgacgaac atctcccact cggcgctgac
    15721 atacttgtcg tactcgctcg ccatcggccc gccaacctcc cgccgcatca gaatgtcgct
    15781 ccgcgtcggc ctaactcacg gctgtaatca ctgccacccg ttgcgtactc cgtgagtaaa
    15841 aaaagcaggc cgctaatccg gtagtcgcct gcgcccccga aacttcgccc cggccgccgg
    15901 ccgcggccac atcctcggcg gggttgtccc cggagcggac tcggcctaca ttcgccgcca
    15961 cagcgtcgcc gcctctctgt ggtgcaggtt aatctgcctt gcgacgcgcc gtcggagcag
    16021 gggcgggcgt ttgcgcccgc acgccgaccg gtagcccgtc cgcgcccttc agacccgccg
    16081 tcccggcttc gtcgccgggg aagaaattac ctggggggta gggcggccag gcaccgctcg
    16141 gcctggcgta gagcgcgtta cccacaaacg cccaggccga ggcgaatcgg ttaagcgccg
    16201 ccgtcccgat ggcgccgccg ttgccgaaga cgccgtactc tccgtgcggg agcaggttgt
    16261 tcgtgaacga gaggtcggcg tgcctgaagc cctccgccgt gtcgaggctt agggcaatcg
    16321 agaccgcccc cctcaccgtg ttgtggtcga acgagacgcc ctccgacccg ttgagcacct
    16381 ggaaggcccg cgccccggca ccgtgcgccc ccaactcgat caggttgtcc gagaccgcca
    16441 cccgccgcag ccggacccgc gtgtcgcccg tatccagccc gcgcaggtta acgccgttcg
    16501 tgacgttgcg gatgacgttg cccgagaagg tgacgtcctc gaccacctcc cagccggcgt
    16561 cgcggaacgt gttgaagatg acggcccacc ccgtctgcgc gtccgcccag ctgttctcca
    16621 tcacgttgcc ggtgacgcgg acccgccggg cgttcttcag ctcgaagagg ttcttgaggg
    16681 cgtagccccg cccgcgccac tccgggcgct tgtagagcgt gttgtttctg atcacgatgt
    16741 cggaggggac caggttcggg atggtcgggg cggcgccgcc gaagaggatg ttgatggagg
    16801 cggcctccag gtagttgttc tcgatcagga aggggccggg gccgttccag ccccccagcg
    16861 cctgcgtctc caccccggcc cacttgatgc cggagatgtg gctgttcgtg accgacgtcg
    16921 aggcgctgtt gagggtgatg cctctttgga cggccgtctg gtcgtcgcgc gcgcgcacta
    16981 acacgcggtc gaactccagg tggtgggggg cgtcgtcgag cgtcttctgg ttctcctggc
    17041 tggcgccctc gcccagttgg atcaggttgt atccctcccc cttcatcgcc gccccctgcg
    17101 tgaactccag gcaccgtagc cgccagtggt gcgacccggc cggcgccctc agcaccggcg
    17161 agacgttggg cgtcgcgagg cgcgccatca gcggcgctga gtcgggcgag acgcgcaccc
    17221 cctccggcag ttccccgcac cgcgccgagc gcaccgtgat gaaggcgtcg cccgccttga
    17281 ccggcagcac gaagttgccc gtgtaggtcg cccccgaaga taggaccacc tcgtcgcccg
    17341 aggcggcagc gtctaaggca cgctgcaagt tgtcaccggg tctgaccgtg atggttttcg
    17401 cacccgggtt gacgacccgc gggcgggcga cctgatgctg tgcgaatgta taacccaggt
    17461 acacgagcgc gagggcggcc gccaccgaca gcgacgcgat cagcgctttc ttcacctctt
    17521 gctgcttcat aaaacccggc gcccccaacg ccccatcgcg cgtcccgagg aaaccgtcag
    17581 agctacatgg cgagtgcgtc gcgcgccggg gtcggctgcg cggcctgctc attcatgatc
    17641 ggacggagct gataatcgcg gtactgcccc ggctccccct gccggccgtc caccgccgcc
    17701 gccgctatgt tgaaagcctc gcgggcgctc gcgaagtgaa tcttgtagcg ccccgtccgg
    17761 gccgactctt ccagggcctc cgaccagaag cggcgcgcgc cttcaccgat gcacgcgtcc
    17821 tgatcgtggg tgaagaagcc gtggcagtag agcttgagga agacccactc cgggcggccg
    17881 agcaccgaaa tgcgtgcgcc ggcccagcgt tcgaggcgct cgaagtccga ggggtagttc
    17941 gcggccatca cgccgtcgtc gagacgcggc acgggcaggc cgcgcacgcg ccgcgaccag
    18001 ttgaagacga gcggccccgt caggataacg ggcaactgtg gccggcggcc gacgcgcagg
    18061 gacggccccg tgcggtgtgg cacggcctct tcgagcggtc gcccacattc gtagatggcg
    18121 ttgaggcggg gcacctgcgg ttgcaggggc gcggccggca gcgtcatgtc ggcgtagcag
    18181 cccgtctcgg cgagtacgcg catctcgtta tcgacgccgc agaagcgccc gcctgccgag
    18241 ttcgccagag cgaggttgcc gtggacgaag gcgtagcgcg gcggcccgcc cggcgtctcg
    18301 cgcgagaggc acccgtgctc ttcggccaac aggtcgcgga actcgacgag cgcgcggcgc
    18361 aggcccgcct ccgtgtcggg cgtctcgacg ccgtggtgga gatgcacctc gacctcgccg
    18421 aagccttcgg cctgcaagcc ggccatcgtg tcgaggatgg gcgcgtggta ttgctcggcc
    18481 gggtagaagt ttgtgtggcg gtagggcgtg ccgtcatggt cgcggacggc cgcgccggtc
    18541 gcgcgcgccg tctcgcacca ctggtcgagc cgccgccgtt gctcgtcgag cgggaggaag
    18601 ccgccgcgct cactccatgc cggctcgaag tggttggcga cgaccacgat caggtggagg
    18661 ggtgcgccgc cctcgcgcgc gcgccgcgcc gccgcgcccg cgcgccagaa cgggtatcgc
    18721 gccagccacg gcagcgacca ggagagcttc gccagcagtc ccgcgtcgtt ctcgctcatg
    18781 tcgtttgtgc cccgccgtcg cgtacgaaga cgaactcgtt gcagcccagg ccgacgccgc
    18841 cgcacttaag cctggtcagc gtgaatcccc gcgcgcggta gaagtcgaag atttcgtcgg
    18901 gcgtcgccac ctcgtagggg tagccgccga cccagtcgat gatgtcgtgc cagcgggaca
    18961 tcccccggtt ccggtcgtag tccgtccagc gtctgacgta ctcctgcggc cgcagcgtga
    19021 cgagagagcg cagggccggc ttgatttcgt cgggcgcgac ggccagcgcg gcgaagggcg
    19081 ggcgcaggaa gcgcggcagg cggttgtagg ttttcttgat ccacttccag cgcgcggcct
    19141 ggctcccggt gtcgttgtag atggcgacga acaggcggcc gccgggcttg accggcagtt
    19201 gtgcgttctc cagggccagc cacatctggc ccgtgtggtg gagcacgccc cacgagtaga
    19261 ccacgtcgaa gaggccgagt gacctgatgt agtcggcgtc gagcgccgag ccctcctcga
    19321 ccctccacgc ggggtcttcg gggaagtagc ggcggcgcaa ctccgccgtg caggcgaccg
    19381 agcgcgggtc gtagtcgagc gagtggacgc gcgcgccgag gcggcgcgcg gcgaggctga
    19441 aaaggccgct gcccgagccg atgtcgagga agctgcggcc cgcgagcgac tcgaccccca
    19501 gcatcagacg gagcgactcc tcggcctcgc gaatcctgcc gtcgtcgagc gcggcgagga
    19561 agcggctcca attgtcaccg aactcgaacc gctcgccgcg cgcgacctcg cgggcatgtg
    19621 aactcatctc catatctctc ccgcaccacg cctagcgata cttccaataa tagtagagca
    19681 atttcgggta ctccagcgcg accgcccccc agccgctccg gtacagccac caggtggcgg
    19741 ccggcggcgt ctgcgccccc tcctccactg cctcgacgcc gacctcgacg ccgctaccgc
    19801 ggaaggcgcg gcgcagcgtc cacagcgcgc gccgggtgtg gtacgccgag gtcacgaaga
    19861 gcagggagcg tgcgccggcc cgctcggcgt atgcgcgcgc ggccaacgcc tcgtcgtgtg
    19921 tgcccgcgac cgcctcgggc agcacctcga tgctctccgc cgggacgccc gcgcgcagca
    19981 actcctcgcg cgcccgctcg acgaagagcg ggttggtctg tagcgcctcg gaccagccgc
    20041 ctctccggtt gtcgttcgtc aggatcacgc gcggcgcgcg gcgcgcgtgg taaagctcgg
    20101 cggcgcgccg cgagcgctcg acgtaggccg ccgacccgct cagcacgacg agcgcgtcgg
    20161 cgcgcgcgat gccggccgtc tcgaccagcg ccagggccgc cgcccacgcg accgggggcc
    20221 acgcaatgac cagtaccgcc accacggccc acgcctgccg gcgccgcccc gcgcgcccga
    20281 actttacacc acgttgtcgg gcggcactac tcatcgcggg acttcgacgc cagcagctct
    20341 tcgtagagcg cgaggacggc ttcgaggttt tcgaaaccca gcacgtcgcc gggcgttcgc
    20401 cgggcgccct cccgcaggca ctcgtcggcg gccgcgcaga gccgcgcgag gtcttgcgcg
    20461 gggacgacgc gcacgcctgc ggggcgcggc gcgcggtcgg tggcgacgac cgggacgccg
    20521 aagtgcacgg cctcgcgcac ggcgatggag tcgccgtcgt agagcgtcgt gcgcagcatc
    20581 aggtcgctgt cggcgatgac gcgaagggtc acggcccgct ccacgtcgcc gcacaccagg
    20641 acatgctcgc cgtatgactt cgcggccacg ctgcgccgca aatcctcttc gaggctcccc
    20701 gagccgacca acacgagtcc cgcccgcggg aacttctcgc gcaggcggcc gagggcctcg
    20761 atctggagcg gcaggtcgta ctcgggttcg agcccgccga cggagacgag tacgggccgg
    20821 tggcccgcga agaaggagcg cagcggcccc gggggctgga cggcgggcgg ggcggcgggg
    20881 agcgcgtaag ggcttatgag gcgtacgcgg cgggcctcaa cgccgacctg ccggaaccat
    20941 gcggcgacct cctcgttgac cgcaacgacg cggtcgagac gcccgagcgc caggccgcgt
    21001 aaggagcgag gccgcgcggc gcgcccctcg ggcgacgacg ggtagccgcc cgaatgaaac
    21061 gtcagcacgg tcctgcgtcc cggcagcgac ccgcagagta gcaagagcgc ggcgaggcgc
    21121 gcgctgaggt tgccgccgac gtgcaggtgc gccacgtcgt agcgcagcgt caagaggaga
    21181 cgcgcgacgc caagcccgcc gcttgggccg tagacgcccg cgccttcgtc tcggcggttc
    21241 gggctcaggt cgatgacctc gcaggcgacc ccgcgcccca agaggtattc gcgcagcgcc
    21301 gtgacgtgcg cctgcacgcc cccgcgcggc ggcgagtaag gcccgatcaa aaggactctc
    21361 aggggcggca tcgtcttggc ggggtcttta cgcgcgggcg cggggtgcga gcgccgcgag
    21421 gtgttggcgg atttcgtcga gccgcgcctc ccagctctcg gggcggatgg cctcgctgcg
    21481 cgcgcgcgag gggccggggt ctttgagcgc ggcctcgacc tcgcggatgg tctcgcgggg
    21541 gccgtcggcg atgcggcact ggccgaggac ttcgacctcg gggatgggcg tcgagacgac
    21601 ggggagcccg gccgcgaggt actcgcgcac cttcagaggg ttcgcgttga gcgtcagctc
    21661 gttcaggcgg aagggcatca gcgccacgtc gaagcccttg cagtaggcgg gcaaagactc
    21721 gtagggcttg cgccccagca ggtgaacgtt cggcagggct tcgaggactg aaacgtcggt
    21781 cgtcaccttg cctaagataa ccagcgagcc gtgcttgaag tgcttcgcca cctcggccat
    21841 caggtcaacg tccacccagt cggccacgag cccgaagaag ccgatgacgg ggcgcggcag
    21901 ggaggctatc tccgcgggca ctttcgtctc cgggtcgagc gcgcggctga agtgcgcgaa
    21961 gtcaacgccg tggcggacga gcacggcgtg cgggttgatc ttgcctcgtg actgaaggag
    22021 gcgctccgag cttacgatgg cgagatcggc catgcgcaac agtttctcct cgaagacggc
    22081 catcacctcg cccgagacgc cggtgaaggc cgtgtactcg tcaacgcact ggtagatcaa
    22141 ctgctcctcg ccgagtgtgc cggcgacgac cgcggccggc gggttgaaga cccagttgac
    22201 ggggcggcgg aacccgagct tgcgcatcgc gcgcctgacc tggaggcgga gcaggtggcg
    22261 gttaatcgtg cgcacggcca cgctcccgta agcggggatc gccaaagggc tcaggacgta
    22321 gatattaggc tcggggttgc ggataggggt cgccgccgcg gcgagcttgc ggaaggcgcg
    22381 gcccgcatcg gccttcgaga cggtgggcgt gcggtagccg atggagttga cccagagtac
    22441 gcggttgtcg cgcgcgagca ggcgcatcag gtgcgtcttc gagagcgggt cgccgctcca
    22501 gtcgtggctg aagcagatga tgtcacgccc gcgcaactgc tcggaggcgg gtgggcgccc
    22561 acgactctga ctgtcgctgt ggctcacagg tgtttggctc aaaacttttg actcttcatt
    22621 ctcttgattc cgacgatctt tcacttcgag cgcgtgccga ccttggcgtg aaccttctgg
    22681 tagagggcgt cgcccaggag tcccttcgca aggtcgagca cggcggcgcg cgcgcggcgc
    22741 gcgaacaggg actcggtgcg ggctgcgcgg acgacgtcgc gcgcgccggc tccgcggcgt
    22801 accgccaggc gcgcgaggcg gaaggggtcg ccgccggggg catttgcgcc gacgcggctc
    22861 gtcgccacgg agcgatagcc cgccgcgcgc gctgcggcgg cgacgcgggc gtcccagcgc
    22921 ccgccggggc aggagaagtg ctcgatgcgc cggccggtcc actgttccaa acgctctttc
    22981 gactcggcga gttcgcgccg cacctccgcg tcagcgaggc cgttgagcag gcggtgcgtc
    23041 atcgagtggc tgccgacctc gaagccctgt tcggccaact cgcgcacctg gccgggtgcg
    23101 aggtagccgc ggccgcctac gaagcccgcg acgacgtaga aggtagcgcc gaagccgaac
    23161 tccgagagca gcggcgcggc cgacaccagg tcggtctcgc agccgtcgtc gaaagtgata
    23221 gccacgcgcc gcgccgcgcc cccgccgtcg ccgagggcgc ggccggcgct gacgccctcg
    23281 aagccgtcgg ccttgagcgc ggcgaggtgg ccgcggaagt ccgattcgcc gacgacgtag
    23341 ctcgcgtagc cggggtcgtc ctggcagagc gcgcgcccgg gtcgcgccag ctcgtggtac
    23401 atgaggtagg cggtgccgtg tggtctcatc agaagattct gcggagctgg gccttgacgg
    23461 gcgcggggat tttgaggccg aggcggtcgg ccaccttttt caccttcttg atgagacggc
    23521 ggcggcgcat cgagcgcgag ttgacgtcgc cgaactcgcc gagcagggcc tcgggtagct
    23581 cgcccgaagc gggcaggcgc aggtcgccct tcgccccgac atagctgccg gaggccagcg
    23641 ccgccaagac atccgcgcgc cccggcgcgt cgcactcgac caggttgtag agttcacgga
    23701 actgcttctt ccagtgatag tcgacgccgt agaaggcgag catgccgggc cgccgctcgc
    23761 gcaaccgcgc gagcagcttg aaggtgccgg gccgcggcgc agctcggccg tcgtacttcg
    23821 agttccaggt ctcgatgccg cgcggcaaga cctcgaacgt ttcgatccat tcgaacatcg
    23881 agtccatcgg gtgcgcgatg acggagacgc cgcccgcgcg ctcgatgtgc gcgatgacct
    23941 tctgcgggtc ggtcgtcccg gcgagcgtcg tcacgccgta gccgaggacg tgcatgcgcc
    24001 gctcgcactc gtattcgagt ccggggatga agcggaagtg ctcgtcggag agcgcctcgc
    24061 actccgcgac gtaggcgcgc agccgctcct cgtcgaagta ctcggcgtgg tcggtcatgc
    24121 aggcgaagga gcagcccgcc gcgacgagca cctcgcgcag ttcggagagc gtgtactcgc
    24181 cgtccgagta cgtactatga gtgtgaataa cccctctgag cattgacctc ctcacgccgc
    24241 cccgccaatt ttttgaggcc gccgggcagg cggcggaaag cgaaatcgag ccagtcgccc
    24301 aactccggca aagggtcgcg ccaggcgaag ttgtcgtgga aggtgccggc caccggccgg
    24361 aggaaggcgg cgagcgcggc cgcgcgcatc gggaactcgc cggcgtaacc ggccggcgcg
    24421 ccgcgccaga cctcgatcag gtggcggaag tcgccgagca gccagcggca gcgcacgcct
    24481 tccgcgtatg gcgcctgccg gcccacgtcg ccgcgctcgg ccagctccgc gaccagcgcc
    24541 gggaaatcaa ccccggcgta gacggccagc ggcagcgagt tccagaagcg gccgttgacc
    24601 tccatgaaga ccggcgtgcc gtcggggccg atgcggaact cgaccatcgc cacgccgtgc
    24661 cagccgagcg caccgaggat cgcgagagaa gcctcgcgca ctcgcgcttc gggtcgcacg
    24721 ctcacgcgca cggcgctgcc cgagcccgtc ggccgcacgt cgcgcacgcg ccgatgggcg
    24781 aactcggcgc gcacctcgcc ctcgcgcatc agcgcgaagt agccggcccc cgttccccca
    24841 acgaactcct gcacgagcac ggcggaggcg cgccgcgaga ggtcggcgta agccccctcg
    24901 aactcgcccg cgtcgcgtgc gtagagcggc gcgcccgtcg ccacgacgcc ccccgcggcc
    24961 gagacctctt cggacgagcg cggcttgagc acgaccgggt aacgggcctc gcgcatcacg
    25021 cgccgggccg cgtcggcgtc cgagacgagc gccgtgcgcg gcacggcgac gccgagcgcg
    25081 gcggccagcg ccgtcgtctc ctgcttgtcg aaggcgcgcc tgacggttgc ggccggcggc
    25141 aggacgaggc gaccgccggc ctcgaagatt tgcccgcggc gctcggagag cggcagcgtc
    25201 gtgcgctcgg tcatcgggag cacgagcgtg cccggctcgc gcgcggcctc ctcggcgacg
    25261 cggcggacaa agtcagccgc gtcggcctgc ggggccgggt agcggaatgt ccccgacgcc
    25321 ccgcgcgacc acccggcctt cgaccacgaa gcggacgacc cgacgagcac gcggtggccc
    25381 gcgcgcgcca gagacctcac gcacgcgacc gcctgattct cgttgccgtc gagcactaac
    25441 actctcatag cttcacgact gaatggaagt gacgagttcg ggacggcgga tgagagattc
    25501 gccgtccgcc gtcccatctt tcaaacggcg acggcggccg gctcggcgcg cgcctcgcgg
    25561 gcgtcgaaga gttgttcgcc gtcgatgaat tgacgcagcc agacctcgaa gttgacgagc
    25621 gaccagaggc gcgacgagtg gtcgcgcagg cccgcgacgt gttcggaggc gagttggccg
    25681 acgaagtccg cctcgaagat gccgcgcgcg cgggcgcgct cgccgaggac gtattcgtcg
    25741 agcacgccgc ggtggcggcc gcgcagccac gcaccgaagg ggacggggaa gcccatcttg
    25801 ggccgcgtca ggatgggctc gggcagcacg cccttcatcg cgcggcgcag cacgtacttc
    25861 gtcgtccacc cgcgcagctt cagacgctct ggcagccgcg ccgagaactc gacgagcttg
    25921 tggtcgagga acgggacgcg gctctcgatg gacgcggcca tgctcatctg atcctgcttc
    25981 atcagaagct cgtgcaggta ggtcttggtg tcggcgtaga gcaggcggtt gagcaggctg
    26041 tcggcgtcgg tctcttcgac gaggcggcgc acgcccgcgt aagggtcggt cgagccggcg
    26101 cgctcggccg tctcgggcgt gagcagacgg cgttgcatgg cgcgcgggaa gacggcgaag
    26161 ttgtcgaagt acaaactttc gagatcggcg ggcaggcaga ggaaggtgcg ccctagtttc
    26221 tgccggacgc gcgagccggc gggcaggccg gcgacggctc gcgcgacggc gcggcgcagg
    26281 ggcgccggcg cgagcgtctc gtagccgcgc ccgagcgcga ggttgtagac cgtcttgcgg
    26341 tagcgcgcgt agccggccag cagctcgtcg ctgccctcgc ccgtgagcac gaccttaacg
    26401 tggcgctggg cgaggcgcga gacgaagtag agggggatgc tggcgaggtg cgccagcggc
    26461 tcgtcctcgt gccagacgag gagcggcagc gcgtcgaaga actcatcagg gctgaccacc
    26521 acctcgtggt ggtcggtgcc gaacgccccg gagaccatcc gggcgtattc gagctcgttg
    26581 gcctcgcgct cgttgaaggc gacggagaaa gtcttgatcg gctcatccac cagcttgctc
    26641 atcacggcgg cgatggccga cgagtcgatg ccgccggaga ggaacatgcc taagggcacg
    26701 tcggccatca gccgcaggcg caccgattcg cggaacagct ccgaccactc ctcgatcaag
    26761 tccgcctcgc ggcggccttc gttcccccgg ccgtcgtctg agaaggtcac gtcccagtag
    26821 cgctcgactt tcacctcccc gtcgcgccag agcagcgtgt ggccggggag caggcggcgc
    26881 accccccggt agagcgtctc ctcgcccgag ggcgcgtggt tggcgaggta gtcgggcagc
    26941 gccgcgtagt tcagctccgc gcggacggcg cccgcctcca gcagcgcctt gatctccgag
    27001 gcgaagtaga gcgagccgtc ctccgtcagc gcgtagtaga gcggcttgac gccgaggcgg
    27061 tcgcgcgcga tgaagagttc ccggcggcgc gcgtcccaga tggcgaaggc gaacatgccg
    27121 cggaggtctt cgacgcaagc cgcgcccttc tcctcgtaga ggtggaggat cgtctcggtg
    27181 tcgcagtggg tggcgtagac gtggccgcgc gcttcgaggc cggggcggtg gtcggcgtgg
    27241 ttgtagactt cgccgttgta gacgatctgg agcgagccgt cctcgttcgt catcggctgg
    27301 tggccggcgg ccacgtccac gatggcgagg cggcggtggc cgaggccgac cgagccctcg
    27361 acgaagaggc cgcagtcgtc cggcccgcgg tgcgtgagca cgtcgcgcat gcgttcgacg
    27421 agcccggcct cgacgcgccg tcccgacctg ctcgacagag ctatcccgtt aatgccgcac
    27481 ataatcttta aagccctcga cgtgggtgag gcgtctcgac cgcccgcgcg gccgggccgc
    27541 gcccctcctt tgacaggtta tcacgcccct tgggcgcgcc gccaactgat actaaatcgg
    27601 gcggggtgac gccgtctgta ctctcgccgc cctccgactc cgtctcgaac tccagccggc
    27661 cctcgaccgt gcgcgcgacc gcgcagcgca agcgccgcgg cgaggccagc agctcgcgcc
    27721 cgccgaagtg gaagcgcgtg ccgccgccga cgagcaccgc ctccgccaga ctcccgtcgg
    27781 ccgcgagccg cgcccaagtc cacgcgaagt ccgtgacgag ctgccccgcg gagaccgtgt
    27841 gaccggcccg gccgatgacg aggaggtcgc gcgcgccgcc caacgcgact tcgagcgagt
    27901 gcgcggcccc gccgtcgtgc gtcagctccg ccacgcgcgc ggcttcggcc ccggccgcgc
    27961 gcggcaggac gacggtcagg aaatcctgcg ggccttcgcc ctcggcctcg aaagtccaga
    28021 ccggggcggc ggagcgcgag ccgtaacagg tcgagaccca atcctcttcc gcatgccagc
    28081 ggccgccccc gccgtgagcg tgcagctcga cgcccggcgc cgacgagtcg agcaggcgga
    28141 cggccgagcg cccactctcg acttgcgacg gggcgtcggg ggcgaggtgg aagcgcagcc
    28201 cgtagcggtg gcgcgcgcgc gcgtccgcct cgtcgcggat gatgaagtag tcgcccttga
    28261 tgaagaggac ggagcggcgg tgcgtgacgg ggtcgggcag gcgtcggtag ccgtcgtgcg
    28321 cgccctcgaa gaagtcgaag cgcgggcggc tcagccactc gcgcgcctcg cagcgggcca
    28381 ccgcagacca actgaaggcg ccggccgcga cggaagacga ctcgccgtcc acgacgagcg
    28441 cgttgtgcgc ggccgtgtgg cggaagtggt cgcgccactc gcccgagccg gtgtaggtgc
    28501 aggtgcccga atcgacgagg agcgtgcgcc cccgggcggc gagcgtgaac gagagcgcgt
    28561 cggcgtgcgc gtggccgcag ttcatcgcgc cgtgcgggcc gcagtcgagg agcatgtagc
    28621 tcgactcacg cgcccaaccg tcgcgcatca cgtagtagcc gccgtcgcgg aaggcgcgcg
    28681 aagcttcggc gggcggccgc gcgtcgagcg cgtcgaacac ttgaagcccg tcgagccccg
    28741 cgagccacag ggtctcttcc gacgcaggcc cggcgacgta cttgtagtcg gggcgcgcgt
    28801 ggacggcggc cgccgtcgcg agggtggcgc ggaagtctgc gggcggccgt tcgtcgagcg
    28861 gggcgaggcg gccgccgtcg tcgtcgccgt agaagggggt cgtgccgtcg ggcgcggtca
    28921 ggtgcatgag gtggtcacag agggccgaga gcgccggcgc gacctccgcg tccgagggct
    28981 cgccggccgc gcgcgcgagc agaaggaagt gcgtgtagaa gtcggccgtg tagcgctggt
    29041 agtagctggc ctgctcaaag tagacgccgt cgggcctgac ctgccgtgtc agttgttcga
    29101 ggaggatgcg ccgccccgcc gcgcgccacc gcgcggcggg gcgcaactcc ggcagcgcgg
    29161 cgccgaggta gtagaggccg agcgcctcgc ccgtcaggtg cgtgttcggg ctgtagtagg
    29221 tcgagaggta cgtctccagg tggcggccgt tgaggtagag gtacttcagg gcgcgcgcgt
    29281 aagtgtcggg cgcgaggtgg ggcgagtctt tgaagaagtg aagcgcccag agccatgaga
    29341 ttgagcggaa ggcgacttcg aggctgctca tccagttgac gcccgccttc ggcgggtgct
    29401 gctccatcca cgacgcgagg tgcgcggcga aggcgcgcgc gtgcgcctcg tcgcccgtgt
    29461 gccagtaggc gcgccccagc gtgaggaagt gctggtgtcg gttcagctcc cagatgatct
    29521 tcttgtcgcc ggcctgctcg gcgtccagct cggcgaagcg gctccagtgg gtcatcggcg
    29581 agtgcttgcc cgcgacgggc tcgaagttcc aatcgacggg cttgccgaag gagaggtctt
    29641 tgaggccgag caggtcgaag cggcccgcgg cggcgcgcgc ggcgcgctcc gtgacggcgg
    29701 acgagtcggc gaagcggcgg cgcaattcgg cgcgggtggc ctccgactct tcgaaggctt
    29761 tgaagaaggc gcgcggcgcg cggcggaagt gcgcgagcag gctctcagcg tccgtgcttt
    29821 cggggccgcg cggggcgggc aggagaagtc gcgcgagccc cgcgtctgag ggcaagcgcg
    29881 cgccgacgcc gaaccgttcg gcgcgcgcgg ccagcgcctg agccccgcgc acgcgcagct
    29941 cgccgagccc ccggcccttc agccttttaa gtttcttgag cgcgcccttc atcattcttt
    30001 agtagacatc gacggccgcg ggctcgcggc gtcggaggcg gttcttcaac tgctcgaagc
    30061 cgctcgtgct ctgcacgaag tgcggcaggt cggtctcggc cgggacgcgg cggagcgcga
    30121 gcgggtctgc gccgcgccgc tcgttgagac cgggctcggt cgtcacggcg cagaggtagc
    30181 cggcgcttgc ggtctcgcgc cggacgcgtt cgtcgaagac gccgttgggg tagcagaaga
    30241 gttcgacctc gcgcccgagt tcggcttcga gccgcgcgcg tgagtcgcgc aactcgcgcc
    30301 ggagttcgtc gtcgtcggtc gtcgtcagga tggggtgcga gacggtgtgc gagccgatct
    30361 cgacgccggc cgcgtccagc tcgcgcgcct gcgcccaggt gacggggccg aactccgcgg
    30421 ggggaagggc gggcagcgcc acgccgtgcg cggcggcgat gcgttcgatg gccgactcgc
    30481 gctcgcgcgg ggcgagtcgc ttcagctccg agttgacccg gccggccgca ctgatgcgcg
    30541 tgtcgcgccc cgcgagcctg aggtcgaaag tgcgcccgcc cacttcggct tcgagccgcg
    30601 cgccttccgc ggcgagcgcg acgtggcgca gcttgtcagt ccacatccaa gcgcggccgt
    30661 cgagaaagtc cgtgatgacg aagagcgtgg ccggcacgcc gtgacgttgg agcaggggga
    30721 aagcgacctc gtaggcgtcc gcgaagccgt cgtcaatcgt cacgcacgcg gcgcgcgcgg
    30781 gcaactcgcc cgcgcccgcg tagtgggccg cgacgcggga gagcgggacg agggtgtagt
    30841 gctgtttaag gtagcggagg tgcgcgtcga attcggcctc gggcgtggcg agcgggtcgc
    30901 cgccgcggcc gaagcggtgg tatgtgagga tgagcacgcg gccacggttc gccgcccgga
    30961 agggggcgaa ggctccggcc cgcgtcagca gtttgagcgc cgaatgcttc atcgcccctc
    31021 cgcgtcggcg gcgtgccctt tggcggcggc cgaggcttgg cggcggcgtg cgcgcaggcg
    31081 gcgccgcagc tcgtccttct cgcgcttgac cggcagccga taccgtttgc cgagactggt
    31141 gtcctgcggg cgcgcttcat aagtgcccgc ggcaatcatc ccgacggcgc gcgcgaccag
    31201 ctcggccgag cgggcgcgca ggcgtgcggt gtactcttcg atgaaggctt cgaagtcgtc
    31261 gccgtgcgcg tcgtcgtagg cgagcggcgc ggcctcctgc aaaagaatct cgccggtgtc
    31321 caccttggcc gcgacgaagt ggacggtgag tccgacctca cgctcgccgt tgtagagttc
    31381 ccagaagacg ggcggcccgc cgcggtagaa gggcgcgagc ccctgatgaa gattgatcga
    31441 gccgaggcgc gggaggcgga agaccgactc gcggatgatg ttcgtcccgt agatgacccc
    31501 gaggtccgcc tgcgccgcgc gcagaagctc cgcggcctct tcggtgtggt agttggcgac
    31561 gaggtggaag gggacgccgc gtgctgtgca cgtctcgcgc agcgcgtcgc gaccgacctc
    31621 catcacgtcc gtgcccgcgc ggcggcggag caactgcccc gccagcttcg cggcggtggc
    31681 cgcccagccg tcgtagcgga tggagcggcg cagcttctcg cgccagtccc gccgcggcgt
    31741 ggtctccgtc tcgacgaaga cgcccgccac ctcgacgcct tcgacgccga gcaggcgttc
    31801 gagcacgagt tcggccccgc cgtgcgtcag caggatgacg cggaacttcc tgtcgcttcc
    31861 cttctcagtc atcactttta acagaaccgc gagcggtcgc gagcgggtgc gagttacata
    31921 atgcgggctc taccccgcaa cacgtacccg ctcgctaccg ctcgcggttc tgtttctttc
    31981 taagagttgt tcgtagagcg cctcggtgcg cgcgagttgc gcgtcgcacg agaactgtcg
    32041 cgcgacacgc tcgcgcccgc gctcgcccat ctcgcgcgcg cgcacggggt cgcgcaggag
    32101 tccgccgacg cgcgcggcca tccgttcgtc gtcgccggcc gggacgaggt gtccggtctc
    32161 gccctcggcg acggcctcgc gcgccccgcc cacgtcggtc gcgaccacgg ggcggccggc
    32221 cgccatgtat tcgaggatgg agttcgagaa gccctcggcc gtggaactca gcacgcacac
    32281 gtccgaggcg gcgagcagcg ccccgacctg cgtgcagcgc cccgtgaaga agaccgcgtc
    32341 ggcgatgccc agttcggccg ccagcgcgcg cagcggcgtt tcgagttcgc cctctccggc
    32401 gaggacgaag gcggccgacg gaaactcccc gcggacgcgg cgggcggcgc gcaggaaggt
    32461 cgggtggtct ttgacggcgt ggcgcaggtt ggcgacgagc gtgacgaagc gccgccccac
    32521 gtccgccgga aggcccagcc cggcaagcgc ctccgcgcgc gagaagtcgg cgggcggccg
    32581 gacgcgcgcg gggtcgaggc cgttgtagac gacggcgacc ttgcgggcgg gcacgccgtc
    32641 tcgcactaac tgttccttga cggcctcgga gttggcgacg accgcccgcg cgagacggta
    32701 cgcgatcagc tcgacgcgcc tctgcgccgc ggtgcgcatc ccgccggtct cgcgcttggc
    32761 ggcgacgcgc gcgccgacgc ctgccagcgc cgcgccggcc atcccgaaga cgttcgagta
    32821 gaagtcgtgc gtctgcacga ggtctacgct ttcggcgcgc aggaaggcgg cgaagcggcg
    32881 gagttgtgcg acgaaccgcc ggtcgtagaa ggagttgagc gggtacgcgt ctatctcgcc
    32941 caggttcagc cgctcgacct ccccgcgcag cacgccctct gggttgaggc aggcgacgcg
    33001 gacgcggtag cgccccgact ccgcgagcag gcgcgtgagc tgcgcggcct gccgctccga
    33061 gccgccctgg tggaagctcc ccatcagttg gaggacgttg cggggcatgg ctctcaagtg
    33121 gctatcgctt ccaactcctc cccgaccggc gccgcgtcgg cgtaggcgcg gtgccagagt
    33181 tcgaggatga agagcgacca gagcgcgttc gagtggtcgc ggcgtccgcg ctcgtgctcg
    33241 gcgagcagca cgtcaacata ggccgcctcg gcgatgccgc gtgtccgcgt gcgcgcgtcg
    33301 gcgaaggtgt cgcggatgcg ctcgcgcagc tcgcggttga tccactcgcc gatgggcacg
    33361 ccgaagccct gcttcgggcg gtcgaggatt tcgtcgggga cgagcccgcg cacggcccgc
    33421 ttgaagacgt acttcgactc cgtcccccgc atcttcaaag aggccgggat gcgcgacacg
    33481 aactctatca gcttgtgttc gagcagcggc acgcgcgctt cgagcgaggc ggccatgctc
    33541 atgcggtcaa ccttggtcag gatgtcgccc ggcaggtagg tcttcgcgtc gaggtagagg
    33601 agcgacgcga cccctccgcc cgcgctcgcc cgcgcggcgt gcccgcggaa gatttcggcc
    33661 gccggctcgc gccccccgag cgcccgccgg aagtcggccg tgtagagcgc gcccttgccc
    33721 agctcgttga aggtcgagac gttgtcgagg tagcgctcgg cggggtcgag cgcgacgttg
    33781 tggaggaagt tgcggccgcg cgcgccgtgc gggagacggc cggcgagcgg ggccatcaag
    33841 ccacgccgca tgaggcgcgg caggcgcgcg aacccctggc gaccctgctc gaccgcgtag
    33901 cgcgtgtagc ccgcgaacaa ctcgtcgccg ccgtcgcccg agagcaccac cttgacgtgc
    33961 tcgcgggcga gcttcgagac gaggtaggtc ggcacggacg actggtcggc gaacggctcg
    34021 tcgaagtgtc ggacgagttc gtcaaccagt tggcagacct cgggcgtgac gatcagctcg
    34081 cggtggtcgg tctcgaaatg acgggcggcg aggcgcgcgt acttcagctc gtcgaagctg
    34141 tcctcgcgga agccgatgga gaaggtcttg acgggcgcgt tcgtggcgcg gctcatcagg
    34201 ccgacgatgg tgctcgaatc caccccgccc gagaggaacg cgccgagcgg cacgtcggcg
    34261 acgagcatca tccgcacaga ctcgtcgagc cgcgcgcgca actcctcgac gtactcctcc
    34321 tcgctccgcg gcgggtcgtc cgtctcgacg gggaagtccc agtaagattc cgtcgtgacg
    34381 cgccccttct ccaaaacgag gcggtggccg gcttcgagct tgcggacgcc gcggaagacg
    34441 ctcaggggct ccggcacgta gccgagcgtg aggtaggcgt cgagcgccgc ggggtctgtc
    34501 tcccgagcga cccccgggtg ttcgagcagg cacttcagct ccgagccgaa gacgaacgag
    34561 ccgccgggcg tcgtggtgta gtagagcggc ttctcgccgg cgcggtcgcg cgcggcgaag
    34621 aggcgttcgt tgcggccgtc ccagacggcg aaggcgaaca tgccgcgcag gcgctcgacg
    34681 cacgcgtcgc cgaactcctc gtaggcgtgg acgattgctt cggtgtcgga gcgcgtgcgg
    34741 aacttgtggc cgcgcgcttc gaggtcgcgc tgcaactctt tgtagttata aatctcgccg
    34801 ttgaagacga tgcggacgcg ctcgtcgcag ccccgcatcg gctggtgtcc gcccgcgagg
    34861 tcgatgatcg agagccgccg catgccgacg gcggcgcggc cgaggacttc cgccccctgc
    34921 tcgtccggcc cgcgatgagc catgacgcgg cacatacggt cgatcagcgc ggggcgcgac
    34981 ggcccctctt caacacccgt cgatgtcggc tcgatgaatc cggcaatacc acacataacg
    35041 tttacttaaa attgcacgtc ggcctcggga acacgtagga ggctgtcttc aggatcgaag
    35101 gcatcgaatg gccctcccta atccacctaa gcgtccccct gtcgatctct taattgtgcg
    35161 ctctggcccc ccgccccacc ctcccggatc ggctcccagc gagcataccg ctcgccgcgc
    35221 gcgaatttaa agaaggcgac cgccgccgcc acattcgcca gtaggaagta ttgcggcagc
    35281 gccagcaacc gtgagtgcag cccggcgcgt tcgagcaaga gccccgccac cgccgcgccg
    35341 tagcagagca cctgcgcgat ggccgccgcg gtgtagaagc ccgagccggg cgcgagcgcc
    35401 agcgaggcgg ccaggcaggt gagcaggaag ccgggcacga ggtagcgtaa tagcttatgg
    35461 gagatgagct ggacggcata aaaaccggag cgcagagggt tgagcatcgc gcggtgccgc
    35521 cagaggtcgg cgagggtctg cgcgatgatt cgcacgcgca tgttcagctc cttaccggag
    35581 cggctgttgg tctcctcggt gcagatggcg gcgggctcat aaacggcccg cagcccctgc
    35641 tcgaccatct tcgtcgcgat gatgaagtcg ctgcacgctt cgtgatagag ggggacgtag
    35701 gccgcacgtc gcaccgcgta gaggcaaccc ggcgcgccga ccagagagca ggcccggctc
    35761 tcgtgccggc ggaggaacgt ctcgtagccc cagtaggaac gcgcgccctg tccggtgctc
    35821 gagttggccg tatcgacata gacgagatgg ccggcgacgc agccgaccga ggcgtccgca
    35881 aagttcggcg ttatggcgcg cagcacgtct cgccggtaaa gggtagtggc gtcggagaag
    35941 aggatcacct cgccgccggc aagttcgacc gcggcgttct gcgcggccgt cttaccgagg
    36001 cgctcgggct gccggtgcag gcgcacgccg cgccgggcga aagagcgcac gatctcgtcc
    36061 gtcccgtcgg tcgaacaatc ggacgctacc aacacttcaa gcttcgcagg gtcgtagtcg
    36121 agcgccagcg tattctcaag cttggcggcg aggtcgcgct cctcgttgta cgcggtgatg
    36181 acgacggtga cggacggctc gtatgctgcg cgcgtccagg gccgcgcccg cacgcgactt
    36241 accacccaca ccagcagggg atacccggca taagtgtaga agagcgcgaa aacgctcgcc
    36301 cacaaaagga tttccgcgac agtagtcatg tggaggggat gcgggccgat tcgatctggg
    36361 ggtggtccaa tttggctccg gaaggctcac tcccggcctc gccgtatttt agcacgtaaa
    36421 ggcggcgcag gcagacggcg taacctacca gatagtatac ataccagagg tgcgcgacgg
    36481 aggcgaaaaa gctgcttacc atgtacccga ttaagctggc ctgcaacccg atggagaggt
    36541 agtagaagcc ggcccggtct ttctccgaaa aagactcacg ctcaattcga cgcagacctc
    36601 tgatagatgt gaccatgaac atgacataga cgaccagggc cgctatgccc atctcagccg
    36661 cgacctgcgt gtaggcatta tgactcacct gctcttgcgc gcccctgtgg tgaaagttgc
    36721 ccaggcccac gccgaagata gggtatctca gcgccaccag tacggaccgc caaagcaggt
    36781 cacgacgggc gtcaaccgag ccggtgatgt caccgccgaa catcgacgtg atccgcgacc
    36841 cgtactcgcc cggcgccacc gcgacaaaga ccgcgaaggt aacggccatc agcgcggcca
    36901 cgaccacccg atttttacga cccagtttcc atgcaacgac gccggtcacg gccactaagc
    36961 cgaggaaccc gccgcgcgag aaggagacga cgatgcccgc gacgaagagc gccgcgcaca
    37021 cgccgtaaca tattttcttc agggcccccc gccgggcgag cgcgagtccc gccgcgagcg
    37081 gcaccatcga gacgaggtgg agggccaggt cgttgggatt ggcgaagagt ccgcccagca
    37141 cgcccttcac gcgttcacca cgcatctcta aaagcccgct ccggtagtca tcgaaggcgt
    37201 tgaaactcac cagcacgctc accgccatcg ccagaatgag catcccgcgg aagcgccgct
    37261 ccgtacgcac cacgttaacg atgacgacga atatggagat gactttaagg aactcgacga
    37321 agttgtccca agcctcgccg gggctgata
//
LOCUS       DASVES010000364         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_834518, whole genome shotgun sequence.
ACCESSION   DASVES010000364 DASVES010000000
VERSION     DASVES010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834518"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1312)
                     /locus_tag="VF240_14970"
     CDS             complement(179..1312)
                     /locus_tag="VF240_14970"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX7176566.1"
                     /translation="MHQIEVATTRETLLDNPEMILNMGPQHPSTHGVLRVILKLDGET
                     VIDLDCDIGYLHRGMEKIAESRLYPMIAPYWDRLDYIAAVSNGLVWVETVEQMIGLEV
                     PPRAHYVRTILTELNRIASHLLWLATHGLDIGSFTVLLWALREREEILKIFEQQFGAR
                     LTCHAWRIGGMPNDTYEGFTEDVQRFIDNFPKRLEEYETLLSENRIWLGRTVGIGIIS
                     AADAIAIGLSGPALRGSGVAWDIRKSRPYAAYAELDFDIPVGENGDTYDRYLVRLEEM
                     RQSRRIVQQALNQLPDGPVMAKVPKVIKPPVGDYYHSIEGPKGEIGCYLVSDGTSRPH
                     RMRVRPPCLINLQALRQMCIGHLVADVVAIIGTLDIVLGEIDR"
     gene            complement(1414..1665)
                     /locus_tag="VF240_14975"
     CDS             complement(1414..1665)
                     /locus_tag="VF240_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176567.1"
                     /translation="MHPKDLAAKHDVKVFDSPEAAAKEGFALGERLTTRNVWNRDSSA
                     SAVLFDLIGKRKRGEVEAVALVLDPFGVTGAYKAVNRES"
     gene            complement(1694..>2520)
                     /locus_tag="VF240_14980"
     CDS             complement(1694..>2520)
                     /locus_tag="VF240_14980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7176568.1"
                     /translation="EVNKDVPGGASPAVGDPTQSPSQSDLEKAAKIAEAKARAAAAKE
                     AGAAAGTPKAPVKKKDEGPKPVDASDNPLVRRLRERLGDGLLGAETFLSQLSIRVERT
                     RNVEACRALRDDAETPFDYLSDVTCVHWPENFEAPFEIVYNLYSINGNERVRVKVNAD
                     AEGGVESMTGVWPAANWMEREVFDLYGVQFQNHPDLRRLLLPPDWEGHPLRKDFPLEF
                     MENNWTTKHLPEFSEVQQEQLEQRRAYGLEILSTADERRIREIFRDGKELLPKEHR"
BASE COUNT          414 a          754 c          846 g          506 t
ORIGIN      
        1 atctcgcgcg cgccgcggct gtcaatcagc atcccgaacg gcgcgaacgc ctcgcgcgtc
       61 atcttctgaa ccgggaggcg gataacattc ataaaagcag tgatgagtga taagtgatga
      121 gtgataagaa agagaagacg gattttgtct tatcacttat cactcatcac ttatcacttc
      181 acctgtctat ctcgccgagc acgatgtcga gcgtgccgat gatggcgacc acgtcggcga
      241 cgaggtggcc gatgcacatc tgccggagcg cctgaaggtt gatgagacac ggcgggcgga
      301 cgcgcatccg gtgcgggcgg ctcgtgccgt ccgagacgag atagcagccg atctcgccct
      361 tcggcccctc gatggaatga tagtagtcgc cgacgggcgg cttgatgacc ttcggcacct
      421 tcgccatcac ggggccgtcg ggtaattgat tcaaagcctg ctggacgatg cgacgcgatt
      481 ggcgcatctc ttcgaggcgg acgaggtagc ggtcgtatgt gtcgccgttc tcgccgacgg
      541 ggatgtcgaa gtccagctcg gcgtaggccg cgtaggggcg gctcttgcgg atgtcccacg
      601 cgacgccgga gccgcgcagc gccggccccg aaagcccgat ggcgatggcg tccgcggcgg
      661 agatgatgcc gatgccgacc gtgcggccga gccagatgcg gttctccgag agcagcgtct
      721 catactcttc gaggcgcttc gggaagttgt cgatgaagcg ttgcacgtct tcggtgaagc
      781 cctcgtaggt gtcgttgggc attccgccga tgcgccacgc gtggcaggta agacgcgcgc
      841 cgaactgctg ctcaaaaatc ttgagtatct cctcgcgctc gcgtaaggcc cagagcagga
      901 cggtgaagga gccgatgtcg agtccgtgcg tggcgagcca gagcagatgc gaggcgatgc
      961 ggttgagttc cgtgagaatc gtgcggacgt agtgcgcgcg cggcgggact tcgaggccga
     1021 tcatctgctc gacggtctcg acccagacga ggccgttgga gacggcggcg atgtagtcga
     1081 gtcggtccca gtagggcgcg atcatcgggt agagacggct ctcggcgatc ttctccatgc
     1141 cgcggtggag atagccgatg tcgcagtcga ggtcgatgac cgtctcgccg tcgagcttga
     1201 ggatgacgcg cagcaccccg tgcgtcgagg ggtgctgcgg ccccatgttg agaatcatct
     1261 cggggttgtc gagcagcgtc tcgcgcgtgg tggcgacttc tatctggtgc ataagaaccg
     1321 tgaatcgtaa atcgtgaatc gtaaatcgtg ctcgctcgtc caagcttgag aggcaaaccg
     1381 cttcgcggcc gtgtgctgac cgcgattgac gatttacgat tcacgattta cggctttata
     1441 agctcctgtg acgccgaagg ggtcgagcac gagggcgacc gcctcgacct cgccgcgctt
     1501 gcgcttgccg atgaggtcga agaggacggc cgaggcgctg ctgtcgcggt tccagacgtt
     1561 gcgcgtcgtc aggcgttcgc cgagcgcgaa gccctccttc gcggcggcct cggggctgtc
     1621 gaagactttc acgtcgtgtt tggcggcgag gtctttcggg tgcatcgtcg tttcggttcg
     1681 gccgaacttt ggtttacctg tgctccttcg gtagaagctc cttgccgtcg cggaagattt
     1741 cgcggatgcg gcgctcgtcg gccgtcgaga ggatttcgag accgtaggcg cggcgctgtt
     1801 cgagttgctc ctgctgcacc tcggagaact ccggcaggtg cttcgtcgtc cagttgttct
     1861 ccatgaattc gagcgggaag tctttgcgga gcgggtgtcc ctcccagtcg ggcggcagca
     1921 gcaggcggcg caggtcgggg tgattctgga attgcacgcc gtagaggtcg aacacttcgc
     1981 gctccatcca gttcgccgcc ggccacacgc ccgtcatgct ctcgacgccg ccctcggcgt
     2041 ccgcgttcac cttcacgcgc acgcgctcgt tcccgtttat cgaatagagg ttgtagacga
     2101 tctcaaacgg cgcctcgaag ttttcgggcc agtggacgca ggtcacgtcg ctcaaatagt
     2161 cgaagggcgt ctcggcgtca tcacgaagcg cgcgacaggc ttcgacgttg cgcgtgcgct
     2221 cgacgcgaat cgagagctgg ctgaggaagg tctccgcccc gaggaggccg tcgccgagtc
     2281 gttcgcgcag gcgacggacg agcgggttgt cagaggcgtc tacgggcttc ggcccctcgt
     2341 ccttcttctt gaccggcgcc ttcggcgtgc ccgccgccgc acccgcctcc ttcgcggccg
     2401 cggcgcgggc ctttgcctca gcgatcttcg cggccttctc aagatcggac tgtgacggac
     2461 tctgcgtggg gtcgccgacg gcgggcgacg ccccgcccgg cacatcctta ttaacttctg
//
LOCUS       DASVES010000365         8890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1224684, whole genome shotgun sequence.
ACCESSION   DASVES010000365 DASVES010000000
VERSION     DASVES010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224684"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VF240_14985"
     CDS             complement(<1..1167)
                     /locus_tag="VF240_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX7176569.1"
                     /translation="MSRVSRLVLAVVFLSLAAIVSGAQQNEEFFPGGTYDPAIPTPAS
                     VLGYEVGTHHTDYAGLERWLAALRPSERVRVVRYGESIEHRPLYLVVISSPANLARLD
                     AVRADMRRLSEARATTAADAARIAASTPAVAWMNHANDGNESAAFESAIQTMYQLAAG
                     TDELTRLVLDRLVVVVNPAHNPESHERFVNWYNSAQVGRTGTADRAAAEHNAPWGMST
                     NNNHYQIDLNRDAFFVTQPETAALVRAFGEWNPVVFADHHGQTKNMFFPPPAEAVNLN
                     VTREQFDWMDKIGHAIGAAFDQRGWSYYRRGRFDLFYAGFWDSWPTLNGAVGLTFETD
                     GGGDKGLAWEREDKTVLTLRDGVLHHHTATMATLRLTAENKEQFLTGLHRYKQSA"
     gene            complement(1625..2119)
                     /locus_tag="VF240_14990"
     CDS             complement(1625..2119)
                     /locus_tag="VF240_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7176570.1"
                     /translation="MHELLEELPASKPEAYRLLAAQLAGLLEGERDLIANAANFSALL
                     YHTLSGLNWAGLYFHKGGELVLGPFQGKPACVRIALGKGVCGTAAESRRTVLVENVHE
                     FPGHIACDGASNSEVVVPLLRGDRLVGVLDLDSPSLARFDAEDREGLERLAEIFLDAT
                     DVDE"
     gene            2333..2569
                     /locus_tag="VF240_14995"
     CDS             2333..2569
                     /locus_tag="VF240_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176571.1"
                     /translation="MIEVQIGLHEESSVELEIFTPQLLALRIMGQSERARTSLLTREQ
                     AVCLRAALDELIAELIPTAPAEPDAAGAARLRAA"
     gene            2660..3427
                     /locus_tag="VF240_15000"
     CDS             2660..3427
                     /locus_tag="VF240_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7176572.1"
                     /translation="MTTGVRADYDPFAWFYDEYWCRGVTGDFLHALERLLLPRLAARA
                     RLLDLCCGTGRIAANLTARGFSVTGLDISAEMLSYARRNAPAAEFVAADARAFSLPPT
                     FHAAISTFDSINHILSLGELAAAFRNVRRSLVGGGVFFFDVNLEMGFREHWREHYSAI
                     EAERVCLVKGYYDAAARLGRYDFTLFRQLNGCWHRSDFSISERCYTKAELRDSLGRAG
                     FSRIKFFDAPTAAGLREHRGRVFILAEKSDRTNDDDD"
     gene            3417..4379
                     /locus_tag="VF240_15005"
     CDS             3417..4379
                     /locus_tag="VF240_15005"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX7176573.1"
                     /translation="MTINFVDFLLALLVLLSIVHGWQRGFLYGLLDLARWIAGLMLGL
                     RYYQPVARWLAANFDWSSIWYKPAAFLMTASAVGIVVQLVGQALLGRLSKDVHEGTTN
                     RLLGTLPGAAGGLVFAAITSALLLAVPLPETLRESARESTSVNRLAALAENLESRLSP
                     IFDEAIAQTLNKLTVQPESGESVELPFKVAVTRPRPELEAEMLELVNRERVAAGLRAV
                     APDPEIAEVARRHSVDMFGRGYFSHVTPEGHSPFDRMNEGGVTYRTAGENLALAPTLT
                     IAHTGLMNSPGHRANILRPEFGRLGIGIVDGGARGLMVTQNFRN"
     gene            complement(5038..5283)
                     /locus_tag="VF240_15010"
     CDS             complement(5038..5283)
                     /locus_tag="VF240_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176574.1"
                     /translation="MYIYTIQAMPGQKAKFEGDTSVKYQVKRVNTEIAASKKGRSEII
                     RTNLEYEDAMRLADGFNDKEGKSQTIREEVARQKNPS"
     gene            complement(5383..8016)
                     /gene="hrpB"
                     /locus_tag="VF240_15015"
     CDS             complement(5383..8016)
                     /gene="hrpB"
                     /locus_tag="VF240_15015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEX7176575.1"
                     /translation="MQPLPIDSLLPEIIESLRRAPSLVVEAPPGAGKTTRVPAALLDS
                     GFLGDGGEVWVLEPRRMAARMAARRVAEERGEAVGETVGYQVRFEDVSGARTRLRFVT
                     EGVFTRRLLSDPQLSKAGAVVLDEFHERHLQADIALAVVRRLQLTSRPDLRVVAMSAT
                     LDAAPVARYLGDCPALRSEGRLFDVAVEHLARPDERPLHEQVASAVRRLLSEELSGDV
                     LVFLPGAAEIRRAQEACAALAEQHALLVLPLHGELSAAEQDAAVRPADRRKIILSTNV
                     AETSVTIEGVVAVVDSGLARVAGHSPWSGLPALAVARVSQASATQRAGRAGRTRPGRA
                     LRLYTAQDFAARPAHDEPEVRRLDLAEAALELHAAGVRDLGEFGWFEPPPPASLDAAR
                     ALLRRLGAIDERGEVTATGRRMLRLPLHPRLARVVVAADERGVGAEACAVAALIGERD
                     IREREIFQQPRAPRREAVRHGPSDALELLDLFEEAARAGFTPARLRALGLNPSAVRNA
                     ERVRRQLERQMKRRGPESVRREPDTSARESEATAARESALLMSILTGYPDRVGRRRAG
                     GAGAKTEGVEILLAGGGAAELAPESVVRRAEFLVAVDAEERRGAQAGGAGRGARAGAV
                     VRLASAIEPEWLIDLYPDAVRETTEAVWNAEAERVEVVSRLLYEQIVLTEGRAAGASD
                     VASGVLAGAALAAGWDAFAEREAVERFLARVEFAAVALPEENFPSLAEEDVCAALARL
                     CEGRRSFAELREAARAGALFDLLRARLTPEQTRLLARSAPERVSLAGGRQVRVRYGRG
                     AQPWVASRLQDFFGMREGPRVGGGRTALVLHLLAPSQRPVQVTTDLAGFWQRHYPQVR
                     RELSRRYPRHAWPEDPLNPSR"
     gene            complement(8044..8352)
                     /locus_tag="VF240_15020"
     CDS             complement(8044..8352)
                     /locus_tag="VF240_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176576.1"
                     /translation="MHLVQILLPLYDNDGRRFGADAFDRVRNELADRFGGVTAFRRSP
                     AEGLWKDEGGAVARDEVTIFEVMSEELDRAWWGVYRAELERRFQQEKMVVRATEFVKL
                     "
     gene            complement(8448..>8890)
                     /locus_tag="VF240_15025"
     CDS             complement(8448..>8890)
                     /locus_tag="VF240_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176577.1"
                     /translation="PGFFSVNLRASKTIGFGGAPAAAAGGGDAAGQGGRRGGGGGGGG
                     RRGGGGGGRGGGGGGRGGGGGGTLGGEGSGETASRYNLTFSVNVQNLFNRTNLGTPIG
                     NLNSPLFGQSTGGSGRFGFGGGGGGGGQQTAGNRRVELQLRLSF"
BASE COUNT         1419 a         3007 c         2941 g         1523 t
ORIGIN      
        1 ggccgactgc ttgtagcgat gcagccccgt caaaaactgc tccttgttct cggccgtcag
       61 acgcagcgtc gccatcgtcg ccgtgtggtg atggagcact ccgtcgcgca gtgttaagac
      121 ggtcttgtcc tcccgctccc acgcgaggcc cttgtcgccg ccgccgtcgg tctcgaaggt
      181 gaggccgacc gcgccgttca gtgtcggcca cgaatcccag aagccggcgt agaagaggtc
      241 gaagcgcccg cgccggtagt agctccagcc gcgctggtcg aaggcggcgc cgatggcgtg
      301 gccgattttg tccatccagt cgaactgctc gcgcgtgacg ttgaggttta cggcctcggc
      361 cggcggcggg aagaacatgt tcttcgtctg cccgtggtgg tcggcgaaga cgaccgggtt
      421 ccactcgccg aaggcgcgca cgagcgcggc cgtctcgggc tgcgtgacga agaaggcgtc
      481 gcggttgaga tcaatctggt aatggttgtt attggtgctc atgccccacg gggcgttgtg
      541 ctcggcggcg gcgcggtcgg ccgtgcccgt gcgtccgacc tgcgcgctgt tgtaccagtt
      601 gacgaaacgc tcgtggctct cgggattgtg cgccgggttg acgacgacga cgaggcggtc
      661 gaggacgagg cgcgtgagtt cgtcggtgcc cgcggcgagc tggtacatgg tctggatggc
      721 cgactcgaag gcggccgact cgttgccgtc gttggcgtga ttcatccagg cgacggccgg
      781 ggtggaggcg gcgatgcgcg cggcgtcggc cgccgtggtc gcgcgcgctt cactgaggcg
      841 gcgcatgtcg gcgcgcacgg cgtcgaggcg ggcgaggttg gcgggcgaag agatgacgac
      901 gaggtagagc ggccggtgct caatcgactc gccgtagcgg acgaccctca cgcgctcaga
      961 agggcggagc gcggcgagcc agcgctccag accggcgtag tcagtgtggt gcgtcccgac
     1021 ctcatagccg aggacggaag cgggagtcgg gatggcggga tcgtaagttc cgccggggaa
     1081 gaattcttcg ttctgctgtg cgccggacac gatggcggcg agagagagaa agaccactgc
     1141 gagtacaagg cggctgactc gtgacatgaa cgctccttgg aaaagggatg gaaggcggac
     1201 gggaaattac cacggcggcg cggggctggc aataacaatc aacgcgccgt agtgtcttga
     1261 atgcggagtt gatgccagtt atctatgccg gatagactgc cggtgcgaag ccgtgatagg
     1321 ctgctcgcta ctatgagcac cactacactg atcatcgtca tcgtcgtact tctcctactc
     1381 ttcggcggcg gctacggctt ctcccgccgt gggcggtaag agcgacccgg cgagagtcaa
     1441 atcaaccggc ggactagagc cgcccccgcg cttttttgat gagcttcggg gcgggccgtt
     1501 gagagtctgc ccctcgtaat tttcagaaac acagatttaa gttatcactc aacgtgccga
     1561 cgcaatcgat tgcgtcggca cgttgtcgcg ttcatttgaa acttgattcc gccgccgggt
     1621 tcgtctattc gtccacgtcc gtcgcgtcga ggaaaatctc cgccagccgc tccaaaccct
     1681 ctctgtcctc ggcgtcgaag cgcgcgagcg aggggctgtc gaggtcgagc acgccgacga
     1741 ggcggtcgcc gcgcaggagc gggacgacga cttcggagtt cgacgcgccg tcgcaggcga
     1801 tgtggccggg gaactcgtgg acgttctcca ccaacaccgt gcgccggctc tcggccgccg
     1861 tgccgcagac tcccttgccc agcgcgatcc gcacgcaggc gggcttgccc tggaaggggc
     1921 cgagcaccag ctcgccgccc ttgtggaaat aaagtccggc ccagttcagg ccagatagcg
     1981 tatggtagag gagcgcggag aaattggccg cgttggcgat gagatcgcgc tcgccctcca
     2041 gcagccccgc gagctgcgcg gcgaggagac ggtaagcttc gggcttcgac gcgggcagtt
     2101 cttccagcaa ctcgtgcatg gcccctactt taaacgccgc gcacggcccc cgtcgagcgc
     2161 gacggcgtgg gcaacagaac gggcagcgca cccatcgaaa taccgtagag acgcggcccg
     2221 tcgcgcacac gcgcatcggc cgccctactc gaacgactct ccctgcttag acggcggccg
     2281 cgctttgtgg ccgcgcgatc gcaaggggga acagttggtg atgaaggaac aaatgatcga
     2341 agtacagatc ggtcttcacg aagagagcag cgtcgaattg gaaatcttca cgcctcagct
     2401 cctcgccctg agaatcatgg ggcagtccga gcgcgcccgc acgtcgctgc tcacgcgcga
     2461 gcaggccgtc tgcctgcgcg cggcgctcga cgagttgatc gcggagttga ttcccaccgc
     2521 gccggccgag ccggacgcgg cgggggccgc ccggctgcgt gccgcctgaa acctctcccg
     2581 cccgaaccga gccgtcggcc ccgcccggcc gtccgctcgg tttttgcgat tcccctcccg
     2641 ccgtttatat tgtcaggcaa tgacgacagg cgtgcgcgca gactacgacc cgttcgcgtg
     2701 gttctacgac gagtactggt gccgcggcgt gacgggcgac ttcctgcacg cgctcgaaag
     2761 gctcctgctg ccgcgtctcg cggcgcgcgc tcgcttactc gacctctgtt gcgggacggg
     2821 ccgcatcgcc gccaatctca ccgcgcgggg cttcagcgtc accggcctcg acatctccgc
     2881 agagatgctc tcttacgcgc gccgcaacgc gcccgccgcc gagttcgtcg cggccgacgc
     2941 acgcgccttc tcgctcccgc caaccttcca cgcggccatc tccactttcg acagcatcaa
     3001 ccacatcctc agtctgggag agttggccgc cgccttccga aacgtccggc gctcgctcgt
     3061 cgggggcggc gtcttcttct tcgacgtgaa tctggagatg ggattccggg aacactggcg
     3121 ggaacactac tcggcaatcg aggcggagcg ggtgtgtctg gtgaagggct actacgatgc
     3181 agcggctcgg ctcgggcgct acgacttcac gctgttccgc caactgaatg gttgctggca
     3241 tcggagcgac ttctccatct cggagcgttg ctacacgaag gccgagctgc gcgattcact
     3301 cggccgcgcg ggcttttctc gcatcaaatt ttttgacgcg cccacggctg ccggtctccg
     3361 agagcaccgg ggccgcgtct tcatcctcgc tgagaagtca gatcggacaa acgacgatga
     3421 cgattaactt cgttgatttt ttgctcgccc tgctcgtcct gttgagcatc gtgcatgggt
     3481 ggcagcgcgg cttcctctac gggctgctcg atctcgcgcg ctggatcgcc ggcctcatgc
     3541 tcgggctgcg ctactaccag ccggtcgctc gctggctcgc cgcgaacttc gactggtcgt
     3601 ccatctggta caaacccgcc gccttcctca tgacggcgag cgcggtcggc atcgtcgtcc
     3661 agctcgtcgg gcaggcgctt cttgggcgac tgtcgaagga cgtacatgag ggcacgacca
     3721 accgcctgct cggcacgctg cccggcgcgg ccggcggcct cgtcttcgcc gccatcacgt
     3781 cggcgctgct tttggccgtg ccgctgcccg agaccctgcg cgagtcggcg cgcgagagca
     3841 cgtccgtcaa tcgtctggcc gcgctggccg agaaccttga gtcgcggctt tcgcccattt
     3901 tcgacgaggc catcgcgcag acgctcaaca agctgaccgt gcagcccgaa tcgggcgagt
     3961 cggtggagct gcccttcaag gtcgccgtca cgcggccgcg gccggagctt gaggcggaga
     4021 tgctggagtt agtgaaccgc gagcgcgtcg cggcgggcct gcgcgcggtc gcgcccgacc
     4081 cggagattgc cgaggttgcc cggcgtcact cggtggacat gttcgggcgc ggctacttct
     4141 cgcacgtcac ccccgagggc cacagcccct tcgaccgcat gaacgagggc ggcgtcacct
     4201 accgcaccgc cggcgagaac ctcgcgctcg cgccgaccct gaccatcgcc cacacggggc
     4261 tgatgaactc gccgggtcat cgcgccaaca tcctccggcc cgagttcgga cgcctcggca
     4321 tcggcatcgt ggacggcggc gcgcgcggtc tgatggtgac gcagaatttc cgtaactgaa
     4381 aataagctgt tagctgttag ctttcagcca gccaaaggcg tgaaccgaag ttaacgcagt
     4441 gatgaatcaa actctggtcg cgcgtcctcc ggctgaaagc taacagctaa tcgctaaccg
     4501 ctcttgaaaa tagaagaggc ccggtcgcgc tctccccttc gcgaccgggc ctcttcggcg
     4561 aggcgttaaa aacgtctcgc cgtttatcta aacaactacc gtgccagtcg ggcgtgccga
     4621 acggcccctc gccgaaacct cgcttttagg gggttagagc aacgtgcggg acttgccccg
     4681 gccctgtgag catctgtttg tgttaacgat ttcgtcggcg ggccatccga tatgggtagt
     4741 ccggcgggca acaaaaatgg atggtaggtc taatgctctt cggtacgctg tctgtaagca
     4801 gccaccgcct cgatggctcc ctcgtgcatc cgctcggggg tcacatatgt ccagtgcttt
     4861 tcaaactcat cgacgctcat cgccggctgt gcggcccgtt ccttattctt tagccactgc
     4921 tccgcccacc gcctgatttc ggggtcacga ttaagagccg ttcgtgcttc atgcatgctg
     4981 ataggagcgc tcatagttta tgtgtttggt ctttcgtgtt ttttaagagt gatgccgcta
     5041 tgacgggttc ttctgccggg caacttcttc tctgatggtc tgcgatttcc cttccttgtc
     5101 attgaagccg tccgcaagcc tcatcgcgtc ttcgtattcc agattggttc taataatctc
     5161 ggaacgccct ttttttgaag ccgctatctc ggtattgacc cgcttaacct gatatttaac
     5221 ggacgtgtcg ccttcaaact ttgccttctg acccggcatc gcttgtatcg tgtaaatata
     5281 catatcggtt tcctttgtag acaggataac gactgtagac aggataacga cttcatgtag
     5341 aaatgggcaa gtgatttact ggaagataac tcgacaatca cactaacgcg acgggttcag
     5401 agggtcttcc ggccaggcgt ggcgcgggta gcggcgcgag agttcgcggc gcacctgcgg
     5461 gtagtggcgc tgccagaagc cggcgaggtc ggtcgtcacc tgcacggggc gctggctcgg
     5521 cgcgagcagg tgcagcacga gcgccgtgcg cccgcccccg acgcgcggcc cctcgcgcat
     5581 cccgaaaaaa tcctgaagcc gcgacgcgac ccacggctgc gccccgcgcc cgtagcggac
     5641 gcgcacctgt ctgccgcccg cgagcgacac gcgctcgggc gcggagcgcg ccagcagtcg
     5701 cgtctgctcg ggcgtgagcc gcgcgcgcag caggtcgaac aacgcgccgg cgcgcgccgc
     5761 ctcgcgcaac tcggcgaagc tgcgacggcc ttcgcacagg cgggcgagcg ccgcgcacac
     5821 gtcctcctcc gcgagcgacg ggaagttttc ttccggcagc gcgacggccg cgaactcgac
     5881 gcgcgcgagg aaacgctcga ccgcctcgcg ctcggcgaag gcgtcccacc cggcggcgag
     5941 cgcggccccg gcgagaactc ctgacgccac atcagacgcg ccggccgcgc gcccctccgt
     6001 cagcacgatt tgttcgtaga gcagacggct cacgacctcg acgcgctcgg cctcggcatt
     6061 ccacacggcc tcggtcgtct ctcggacggc gtcggggtag aggtcgatca accactccgg
     6121 ctcaatcgcg ctggcgagac gcaccacggc gcccgcgcgc gcgccgcgcc ccgcgccgcc
     6181 cgcttgcgcg ccgcgccgct cctcggcgtc cacggccaca agaaactccg cgcgtcgcac
     6241 gacgctctcc ggcgcaagct cggccgcgcc cccgcccgcg agcagtatct cgacgccctc
     6301 tgtcttcgcc cccgcgccgc ccgcgcgtcg ccgccccacg cgatccgggt agcccgtcag
     6361 aatcgacatc aacaaggccg actcgcgcgc cgccgtcgcc tctgactcac gcgcagacgt
     6421 gtctggctcc cgcctcactg actccggccc gcgtctcttc atctgccttt cgagttgacg
     6481 cctgacgcgc tccgcgttgc gcacggccga cgggttgagg ccgagggcgc gcaggcgcgc
     6541 gggcgtgaac ccggcgcgcg cggcttcttc aaagaggtcg aggagttcga gcgcgtcgga
     6601 ggggccgtgg cgcacggcct cgcgtcgcgg cgcgcgcggt tgttggaaaa tttcccgctc
     6661 gcggatgtcg cgctcgccga tgagcgcggc gacggcgcac gcctccgccc cgacgccgcg
     6721 ctcatcggcc gcgacgacga cgcgtgcgag acgcgggtgc aacggcagac gcagcatccg
     6781 tcggcccgtc gcggtcactt ctccccgttc gtcaatcgcg ccgagtctgc ggagcagcgc
     6841 acgggcggcg tcgagcgagg ccggtggcgg cggctcgaac catccgaact cgccgaggtc
     6901 tcgcacgccg gcggcgtgga gttcgagcgc ggcctcggcg aggtcgagcc ggcgcacttc
     6961 cggctcgtcg tgcgcggggc gggcggcgaa gtcctgcgcg gtgtagagac gcagggcacg
     7021 accgggccgc gtgcgtccgg cgcgtccggc gcgttgcgtg gccgacgcct gactgacgcg
     7081 cgcgacggcg agcgcgggca gacccgacca cggcgagtgg ccagcgacgc gcgccagtcc
     7141 agagtccacg accgcgacga cgccttcaat cgtcaccgaa gtctcggcga cgttcgtcga
     7201 gaggatgatc ttgcgtcggt cggcggggcg cacggccgcg tcctgttcgg cggcagacaa
     7261 ctcgccgtgg agcggaagca cgagcaacgc gtgttgttcg gcgagcgcgg cgcaagcctc
     7321 ctgcgcgcgg cgtatctcgg ccgcgccggg gaggaagacg agcacgtcgc ccgagagttc
     7381 ttcggagagc aggcggcgga cggcagacgc gacctgttcg tgcagcgggc gctcgtcggg
     7441 gcgcgcgagg tgttccacgg caacgtcgaa gaggcggccc tcgctgcgga gcgcggggca
     7501 gtcgccgaga tagcgtgcga cgggtgcggc gtcgagcgtg gccgacatgg cgacgacgcg
     7561 gaggtcgggt cgcgaggtca gttgcagccg acgcacgacg gcgagcgcga tgtcggcctg
     7621 aaggtgtcgc tcgtggaact cgtcgaggac gaccgctccc gccttcgaca gttgcgggtc
     7681 ggagagcagg cggcgcgtga agacgccctc ggtgacgaag cgcaggcgcg tgcgcgcgcc
     7741 cgacacgtcc tcgaagcgca cctgatagcc gaccgtctca cccaccgcct cgccgcgctc
     7801 ctcggccaca cgccgcgccg ccatccgcgc cgccatccga cgcggctcca gcacccacac
     7861 ctcgccgcca tctcccaaaa aaccagagtc gagcagcgcg gccggcacgc gcgtcgtctt
     7921 gcccgcgccg ggcggcgctt cgacgacgag gctcggcgcc cgtcgcagcg actcgatgat
     7981 ttccggtagc aggctatcga tggggagggg ctgcatggct tgaggcgacc atgctaacac
     8041 aggctaaagc ttcacgaact cggtggcgcg cacgaccatc ttttcctgtt ggaagcgccg
     8101 ttcaagctcc gcacgataga cgccccacca cgcgcggtct aattcttcgg acatcacctc
     8161 gaagatggtc acttcgtcgc gcgcgacggc gccgccttca tccttccaca gaccctcggc
     8221 gggcgagcgg cggaaggcgg tcacgccccc gaagcggtcg gccagctcgt tgcgcacgcg
     8281 gtcgaaggcg tcggccccga agcgccggcc gtcgttgtcg tagagcggca ggaggatttg
     8341 aacgaggtgc ataagcggaa tagtgagcag tgagcagtaa gcagtgagca gttaagactc
     8401 ggtatgcgtc ttaactgctc actgcttact gctcaccgct cacttcctta gaagctgagc
     8461 cgaagttgta gctcgacgcg gcggttgccg gccgtttgct ggccgccgcc tccaccgccg
     8521 ccgccgaagc cgaagcggcc ggagccgccg gtggactggc cgaacagcgg cgagttgagg
     8581 ttgccgatgg gcgtgccgag gttcgtgcgg ttgaagagat tctgcacgtt gacggagaag
     8641 gtcaggttgt agcggctcgc ggtctcgccg ctgccttcgc cgcctaacgt cccgcccccg
     8701 ccgccaccac ggccaccgcc gccgccaccg cggccgccac caccgccgcc gcgtcgacca
     8761 ccgccaccac cgccgccgcc acgtcggccg ccttgcccgg ccgcgtcgcc gccgccggcc
     8821 gccgccgccg gcgcgccgcc gaagccgatg gtcttactcg cgcgcaagtt gacgctgaag
     8881 aagcccggcc
//
LOCUS       DASVES010000366         9950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_449932, whole genome shotgun sequence.
ACCESSION   DASVES010000366 DASVES010000000
VERSION     DASVES010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449932"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VF240_15030"
     CDS             complement(<1..534)
                     /locus_tag="VF240_15030"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176578.1"
                     /translation="MPRPKKTAGELNGPGTLQRFAETAETVAATTKKLEKAALLGAYF
                     RELSDDDLARAARYFAGQQFALRDARTTNVGGGVLRDALSEVTGLDVENLRPRYVRLG
                     DSGDMAYEVFLETKAANLRPTLTLAATEQFVELLSGTRGTKSKKALVVEALRNATPLE
                     AKYLVKLLTGDLRIGLKE"
     gene            complement(631..1581)
                     /locus_tag="VF240_15035"
     CDS             complement(631..1581)
                     /locus_tag="VF240_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX7176579.1"
                     /translation="MTGKTPRIVSLLPSATEIACALGLQDALVGVTHECDYPPTVVGK
                     PKLTASHISHESMSSREIDHAVRSQLDGHGSIYSLDEELLRELRPDLIITQELCDVCA
                     VSYKTVEHAAARLFDGQCRVVSLEPTTISDILSNVRTVGELTGREAEAAKVVDQLAAR
                     IDALAARLAGVEHRPRTLMLEWLEPPFAPGHWVPEQVALAGGDHTFGRAGEKSYVTTA
                     EEIRAYAPEAIVLVPCGYYKEDTLRALGSARLPEGWAELPAVREGRVWAVDATAYFSR
                     PGPRVVEGAEILARLLHPEIFGAHGEGEAMRVPAELMKAA"
     gene            complement(1660..1947)
                     /locus_tag="VF240_15040"
     CDS             complement(1660..1947)
                     /locus_tag="VF240_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176580.1"
                     /translation="MPRKDARGERVRVGDTVRVVGVPDLGGMSAECRAESLPVFRHLV
                     GKYKRVVEFDEYGHAWLTFRIRKGPHAGLHWVGIEPWLLRVRRTRAKISRP"
     gene            complement(2047..2319)
                     /locus_tag="VF240_15045"
     CDS             complement(2047..2319)
                     /locus_tag="VF240_15045"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEX7176581.1"
                     /translation="MLFMVIERFKNRDAVAVYRRHREQGRMLPEGLKYVESWVEPDFD
                     RCFQLMECEDPRLIRQWTARWQDLVEFEVVPVVTSKEAAEAMSPLL"
     gene            2587..3276
                     /locus_tag="VF240_15050"
     CDS             2587..3276
                     /locus_tag="VF240_15050"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7176582.1"
                     /translation="MLVDKPTDIARTRPDGSPLKPITEVLLERRATNKFTDEEVSAEV
                     LDAVLRLGAQAPSGYNFQPWRFVVVRDAESRRRLQQVAFNQPKVAQAPVVIIAVGMKE
                     EWKETADEVLREGARRGAGNPDKVEQARKGAVQFISGMDAGLWVNRHTMIAVTTMMLV
                     AEAYGYDTAPMEGFDAAGVKREFAIPEEGEVVCLLAIGRAAEPDKPYTGRFPVERIVF
                     AERFGDPWRDK"
     gene            3273..3689
                     /locus_tag="VF240_15055"
     CDS             3273..3689
                     /locus_tag="VF240_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176583.1"
                     /translation="MSSESFDRNMEIVAAHLASLTVKQEQLQESQARAEARLDRSEEQ
                     FKRSSERLDRVERVLGSAIRLGSRQLKETRESVNALIAAQERTTEALARLADSQARTD
                     AKMAETDERLNILVNTVERYISERRNGGGNGSAGGQ"
     gene            3774..4145
                     /locus_tag="VF240_15060"
     CDS             3774..4145
                     /locus_tag="VF240_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176584.1"
                     /translation="MHYAETRFEGAVDHIAVKSDDLPRDVEEYKRMGFRLETLYDDWA
                     MLRDARGFGVALLAPGSKHPPHMGLRVETREALEEFARREGRPLKEHRDRTVSFYTRG
                     AGGQSVEIIYYPPDYKKDGDK"
     gene            4216..4371
                     /gene="tatA"
                     /locus_tag="VF240_15065"
                     /pseudo
     CDS             4216..4371
                     /gene="tatA"
                     /locus_tag="VF240_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            4768..5856
                     /gene="tatC"
                     /locus_tag="VF240_15070"
     CDS             4768..5856
                     /gene="tatC"
                     /locus_tag="VF240_15070"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7176585.1"
                     /translation="MASTLLESGRDNDERDEPEQRLGGQMSFLEHLDELRRRLVRSAL
                     FIAVAFCFCWYFSDYVYNFLSVPVRQALAEAQRRPVPLQGLTGEETILPLSSVKEGDT
                     GRYVFGEAIKLGSSVIPAGASVQVRVARDRSEQIGVFTDEAVIAGVTVIPRGVRLPLD
                     LQASPDALPGIEDKLIVTTAMEPFSLYLQVALYAAIALSVPFLLLQVWGFVAPGLYPH
                     ERGYAIPFVLLSTISFVLGAAFAYYVLFPPAARYLLGLGQDFRLLLKATDYFDFIVLI
                     MLAMGVVFQMPAVTYVLARIGLVTAALLVRSWRISLVVILVAAAVLSPTSDVLNMMLF
                     AAPMAVLYLVSILIAWMFGRQRQTEVRG"
     gene            6193..7842
                     /locus_tag="VF240_15075"
     CDS             6193..7842
                     /locus_tag="VF240_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX7176586.1"
                     /translation="MARHPWMRSLALAFSLAASLQPAALAQQKDKRTADQDPLERERN
                     VKKELREVYKKWLEQDVTYIITDEEKRAFRKLATDEEREQFIEIFWRRRDPDPDTDEN
                     EYREEHYERIAYTNQHFASGIPGWKTDRGRIYITFGPPHGKESHPMGGSFDRPMHHGG
                     GSTSVYPFETWFYRHLPGVGSGIEIEFVDPTGTGEYRLARSPDEKDAMLHIPGAGLTL
                     SEQLGLSDKSDRVSRYGSFGYTGPWARVQDSPFDYLQRITQLSKPPSVNSQLEKSLLA
                     ESSTGAVEDDPLRFDLRIDFFKQDEGRVVTAFTVQTENRELSFKDVGGIQTARLNIFG
                     RVMSVAGRKVASFEDPVTTVATVEDLEAKRDQKSAYQRLVALTPGTYKIDMIVRDVTS
                     GKQHVASHGFTVPKYDAKLLATSTIVLAARLHGLEGQPPVGMFTIGNYKVIPNVSGTF
                     KRGQEVGIYAQIYNAGIDQTTLRPSVDVEYALVRDGKDVYRQTEDWQGLSDAGQRLTL
                     ARLLPTDQLTPGSYEIAIRIRDRVSGQTLTPVSKFTVTAEK"
     gene            8117..9700
                     /locus_tag="VF240_15080"
     CDS             8117..9700
                     /locus_tag="VF240_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7176587.1"
                     /translation="MRQPATRFALLSFVLLVFALPAYAQEDLDARLKEIDEYAAKAGR
                     DWKAPGFSVAIVKDDRVVFVKGYGVRELGKPDAVDADTIFAIGSNTKAFTSAALAVLV
                     DEGKVSWDDPVTKHLPGFQLFDPYATREMSVRDLLSHRSGLATFAGDLLWYESSYGRD
                     EIIRRARHLRPASSFRSRFGYQNIMFLAAGEIVPAVAGKSWDDFLKEKFFDPLGMRRT
                     LTRHADFARASNVAAPHNEVDSRLRSIPHGNVDNVGGAGAIKSSAADMAQWVRLQLAR
                     GTRDGRKIFGTAASREMWTPHAVLGAVSESAEKFNPTTHFGLYGLGWILNDYQGRLLV
                     SHGGGLDGMISRVALMPEERLGLVVLTNSETSLSSALSNKILDVFLGVARRDWSADFL
                     ARTKQAKETSEAAARKVEDARVPNTKPSLSLESYAGVYTGEMFGDARVTAEGGRLVLR
                     LLPSPKFVGDLEHWHFDTFRVRWREGVVYPFPRGFVTFTLDAQGKPAELKLDVPNFDF
                     DFKEIELRRAPDAQTPRSN"
     gene            9729..>9950
                     /locus_tag="VF240_15085"
     CDS             9729..>9950
                     /locus_tag="VF240_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176588.1"
                     /translation="MHKEPQRRINDTLARGVRLRCPACGRASIFETPFRRRPACPSCR
                     VSFEREEGYFVGAISINLVTTEAALLIVYL"
BASE COUNT         1701 a         3488 c         3050 g         1711 t
ORIGIN      
        1 ctccttcagc ccgatgcgga ggtcgccggt gagcagcttg acgagatact tggcttcgag
       61 cggcgtcgcg tttcgcagcg cctcgacgac caacgctttc ttgctcttcg tgccgcgcgt
      121 tccgctcagt agctcgacga actgctcggt cgcggcgagc gtgagtgtcg ggcgcaggtt
      181 cgcagccttt gtttcgagga agacttcgta agccatgtcg cccgagtcgc cgaggcggac
      241 gtagcgcggg cgtaggttct ccacgtcaag cccggtcact tcactgagcg cgtcgcgcaa
      301 aacgccgccg ccgacgttcg tcgtgcgcgc gtcgcgcagg gcgaactgct ggccggcgaa
      361 gtagcgcgcg gcgcgcgcca ggtcgtcgtc cgacaactct cggaagtagg cgccgagcag
      421 cgcggccttc tcaagtttct tagtcgtcgc cgcgaccgtc tcggccgttt cggcaaagcg
      481 ttggagcgtg ccggggccgt ttagttctcc ggcagttttt ttggggcgcg gcatggcggg
      541 attatcaacg gcggcggcgc gggatgtgtg cgcgcttcat tgttttttcg tagacgtttt
      601 tgacgcggcg ccacttgcct cgaaaagcaa tcaggcggcc ttcatcaatt cggcggggac
      661 gcgcatggcc tccccttcgc catgcgcgcc gaagatttcg gggtggagca ggcgcgcgag
      721 gatttcggcc ccctcgacga cgcgcgggcc ggggcggctg aagtacgcgg tcgcgtccac
      781 ggcccacacg cggccctcgc ggacggccgg aagctccgcc cacccttccg gcagacgcgc
      841 acttccgagc gcccgcagcg tgtcctcctt gtagtagccg cagggcacga ggacgattgc
      901 ctcgggcgcg taggcgcgaa tctcttcggc ggtcgttacg tatgatttct cgccggcgcg
      961 cccgaacgtg tgatcgcccc cggcgagcgc cacctgctcg ggcacccagt ggccgggcgc
     1021 gaacggcggc tccagccatt cgagcatcag cgtgcgcggc cgatgttcga cgccggcgag
     1081 tcgggccgcc agcgcgtcga tgcgcgcggc gagctggtcc acgaccttcg ccgcctccgc
     1141 ctcgcgcccc gtcagctcgc cgaccgtgcg gacgttggag aggatgtcgc tgatcgtcgt
     1201 cggctccagc gacaccacgc ggcactggcc gtcgaacaga cgcgccgccg cgtgctccac
     1261 cgtcttgtag ctgacggcgc acacgtcgca cagctcctgc gtgatgatga ggtcggggcg
     1321 cagctcgcgc aacagctcct cgtcgagact atagatggag ccgtggccgt cgagctgcga
     1381 gcggacggcg tggtcgatct cgcggctcga catcgactcg tgcgagatgt gactggccgt
     1441 aagtttcggc ttccccacga ccgtgggcgg gtagtcgcac tcgtgcgtga cgccgacgag
     1501 cgcgtcctgt aggccaagcg cgcaggcgat ctccgtcgcc gagggcagca gcgaaacaat
     1561 gcgcggcgtc tttcctgtca tcgaacaccc ctccggggca cttgttttaa ctttcgtcca
     1621 cgagacccgg cgcggaggcg cggccgatct caacgctcat cagggccggc tgattttcgc
     1681 gcgcgtccgg cgcacacgca acagccacgg ctcgatgccg acccagtgca ggcccgcgtg
     1741 cggccccttg cggattctaa acgtgagcca cgcgtggccg tattcgtcga actccacgac
     1801 ccgcttgtac ttccccacca gatgccggaa gactggaagc gactccgcgc gacactccgc
     1861 gctcatgccg ccgaggtcgg gcacaccgac gacccgcacg gtgtcgccca cccgcacgcg
     1921 ctcgcccctt gcatccttac gcggcattcg tcgcatgtct tagccgacct ggaagataga
     1981 aagcacgctg agcgtgcgcc tccgccgaaa gccgttgccg gattgcggcc cgcggtctga
     2041 tgaatctcag agcagtggag acatcgcctc cgcggcttcc ttcgacgtga cgaccggaac
     2101 cacttcaaat tccaccaggt cttgccagcg ggccgtccac tgccggatga gccgggggtc
     2161 ttcacattcc atcaactgaa agcacctgtc gaagtcaggc tcgacccaac tctcgacgta
     2221 tttcaaaccc tccggcagca tccgcccctg ctccctgtgt cgccggtata ccgccaccgc
     2281 gtcacgattc ttgaaacgct cgatgaccat gaacagcatc ccatcccctc tccggtataa
     2341 gcctaccgac ctgcgcctca gtaaggcaat caggagcaca cgcgccgccg cttatgcagg
     2401 cgccggccat ccgacacact tttagcgccg cccacggcaa aactcaaatc aacgagagcg
     2461 gcgcgcgtcg tccttcggga tcgccccacg cgccggccgc cactccaccg caagtttcgc
     2521 actcgcgcga cgagagggta caatcttccc gccattaaaa ctccgacacg gccagaagat
     2581 tgacacatgc tcgtagacaa gccaaccgac atagcgcgca cgcgccccga cggctcgccg
     2641 ctcaagccga tcaccgaggt gctgctcgaa cgccgcgcga cgaataaatt caccgacgag
     2701 gaggtgagcg ccgaagtcct cgacgccgtc ctgcgtctcg gggcgcaggc cccgtccggc
     2761 tataatttcc agccgtggcg cttcgtcgtc gtgcgcgacg ccgagagccg ccggcgtctc
     2821 cagcaggtcg ccttcaacca gccgaaggtc gcgcaggcgc ccgtggtgat tatcgccgtc
     2881 gggatgaagg aggaatggaa ggagacggcc gacgaggtct tgcgcgaggg cgcgcggcgc
     2941 ggcgcgggca accccgacaa agtggagcag gcccgaaagg gcgccgtcca gttcatctcc
     3001 ggcatggacg caggtctgtg ggtcaaccgc cacacgatga tcgccgtcac cacgatgatg
     3061 ctcgtcgccg aggcttacgg ctacgacacg gcgccgatgg agggcttcga cgccgccggg
     3121 gtgaaacgtg agttcgccat ccccgaagag ggcgaggtcg tctgcctgct cgccatcggc
     3181 cgcgccgccg agccggacaa gccctacacg ggccgcttcc ccgtcgagcg catcgtcttc
     3241 gccgagcgct tcggcgaccc gtggagagat aaatgagcag cgaaagtttc gacagaaata
     3301 tggaaatagt ggctgcccac ctggcttcgc tgacggtcaa gcaggagcag cttcaagagt
     3361 cccaggcgcg tgccgaggcg cggctcgacc gctccgagga acagtttaaa cgctcctcgg
     3421 agcggctcga ccgcgtggag cgtgtcctcg gctcggccat ccgcctcggt tctcgtcagt
     3481 tgaaagagac ccgagagagc gtcaacgctc tcattgccgc acaggaaaga accaccgagg
     3541 cgctcgcgcg actggccgac tcccaagccc gcacggacgc gaagatggca gagacggacg
     3601 agcgtctaaa catcctcgtc aacaccgtcg agcgttacat cagcgagagg cgcaatggcg
     3661 gcggcaacgg ctcggccggc gggcagtagt cagcggcacg gcgcaggacg ttgctgcaag
     3721 catggccgct ttcccgtcgc cgacgccggg cgacatttct gttggaggtt gtgatgcatt
     3781 acgccgagac gcgcttcgag ggcgcggtcg atcacatcgc ggtgaagtcg gatgacttgc
     3841 cgcgcgacgt tgaagagtac aagcgcatgg gcttccggct ggagacgctc tatgacgact
     3901 gggcgatgct ccgcgacgcg cgcggcttcg gtgtggccct gctcgccccc ggctcgaagc
     3961 acccgccgca catgggcctg cgcgtcgaga cacgcgaggc gctcgaagag ttcgcccgcc
     4021 gcgagggccg ccccctcaag gagcaccgcg accgcaccgt ctccttctac acgcgcggcg
     4081 ccggcggcca gtcagtcgaa atcatctatt acccgccgga ctacaagaag gacggtgata
     4141 agtgatgagt gatgagtgat aagagagaaa ctgattttct catcactcat cactcatcac
     4201 ttatcacgct tgattatgtc ttccttcctt gccatcttcg agtttctggg gacgaccgag
     4261 atgctggtga tcgcggtcgt ggcgttgatc atcttcgggc cgcgcaagct gcccgaaatc
     4321 gggcgcacgg tcggcaagag cctcgccgag tttaagcgcg cctccgaaga tttcaggcgg
     4381 acgtgggagc gcgaggtcga gatcgaccgc atcgagagcg atctgcgcgc cgacgtggag
     4441 ggcgccggta agactcccgc actcgacgct gcgacggact ggccgggcga cgccacagcg
     4501 gcggtggcga cggcggggcc ggcggccgac gaagatgcgt ggggagagcg caccattgcg
     4561 cgcgcgcccg gcggagcctt cgagcacacc gagtcgggcg aggtcgaaag cgcgcccatt
     4621 ggagactccg gcccggcgac cggcgacgcc cacgccgaag agtccacgcg caaacacgac
     4681 tggctgtagg acggccccgc gccgtcggca gtagcgccgg cgccgtttgt ttgttcgttc
     4741 gttcgttcga ttctccaact ttccgcgatg gcttcgaccc tacttgagag cgggcgcgac
     4801 aacgacgagc gcgacgagcc ggagcaacgg ctcggcgggc agatgtcgtt tctcgaacac
     4861 ctcgacgagc tgcgccggcg cctcgtccgc tccgccctct tcatcgccgt cgccttctgc
     4921 ttctgctggt acttctccga ttacgtctac aactttctct ccgtccccgt ccgccaggcg
     4981 ctggccgagg cgcagcggcg acccgtgccg cttcaggggc tgacgggcga agagaccatc
     5041 ctccccttga gttccgtcaa ggagggcgac acgggccgct acgtgtttgg cgaggccatc
     5101 aaactcggct caagcgtcat cccggcgggc gcgtcggttc aggtcagggt ggcgcgcgac
     5161 cgttcggagc agatcggcgt cttcacggac gaggccgtga tcgcgggcgt caccgtcatc
     5221 ccgcgcggcg tccgtctgcc cctcgacctt caggccagcc ccgacgccct ccccggcatc
     5281 gaggacaagc tcatcgtgac gaccgccatg gagcctttct cgctctacct acaggtggcg
     5341 ctctacgcgg ccatcgcgct ctccgtgccc ttcctgctcc ttcaggtctg gggcttcgtc
     5401 gcgcccggcc tctacccgca cgagcgcggc tacgccatcc ccttcgtcct gctctcgacc
     5461 atctctttcg tcctcggcgc ggccttcgcc tactacgtcc tcttcccgcc ggccgcccgc
     5521 tacctgctcg gactcggaca ggacttccga ctgcttctga aggcgaccga ctacttcgac
     5581 ttcatcgtcc tcatcatgct cgccatgggc gtggtcttcc agatgccggc cgtgacctac
     5641 gtgctcgcgc gcatcgggct ggtgacggcc gcgctgctcg tgcgctcgtg gcgcatctcg
     5701 ctcgtcgtca tcctcgtcgc cgcggccgtg ctctcgccca ccagcgacgt tctcaacatg
     5761 atgctcttcg ccgctccgat ggcggtgctc tacctcgtct ccatcctcat cgcgtggatg
     5821 ttcggccggc aaaggcaaac agaggtcaga ggttagaggt cagaggtcgg taagaatcag
     5881 ctattactga cctctgacct ctaacctctg acctctgtcg cgggcaaccg gaaggccccc
     5941 tttcggcatc ttgactgtgt tcggtcgagg ctctaaaatc gggtagaaat tcaggcggtg
     6001 cttgggttta cggccccggc ggtgtggagg caaaggaact cttcaggcgt cgccgttttt
     6061 tccggcctcc ccgttcattt cagattcagt ttcatcggcg acgacttccg taacgcggcg
     6121 gcggcagcgg agagtcaaag tcagccgctg ccggtttaat cacttcaatg caattcgaca
     6181 ggagttaaaa cgatggctcg tcatccgtgg atgcgctcac tggccctggc cttctcgctc
     6241 gcggcctccc tccagcccgc ggcgctggcc caacagaagg acaagcggac ggccgatcaa
     6301 gacccgcttg agcgcgagcg taacgtgaag aaggagctgc gcgaggtcta caagaagtgg
     6361 ctcgaacagg acgtgaccta catcatcacg gacgaagaga agcgggcctt caggaagctc
     6421 gccacggatg aggagcgcga gcagttcatc gaaatcttct ggcgacgccg cgaccccgac
     6481 ccggacacgg acgagaacga gtaccgcgaa gagcactacg agcgcatcgc ctacaccaac
     6541 cagcacttcg cctccggcat ccccggctgg aagaccgacc gcggccgcat ctacatcacc
     6601 ttcggcccgc cccacggcaa ggaatcgcac ccgatgggcg gctccttcga ccgccccatg
     6661 caccacggcg gcggctccac ttccgtctac cccttcgaga cttggttcta tcgccacctg
     6721 cccggcgtcg gctccggcat cgagatcgaa ttcgtcgatc ccaccggcac cggcgagtat
     6781 cgcctcgccc gctcgcccga cgagaaggac gccatgctgc acatccccgg cgccggcctg
     6841 accctctccg agcagctcgg actctccgac aagagcgacc gcgtctcacg ctatggcagc
     6901 ttcggctaca ccggcccatg ggcgcgcgtg caggacagcc cgttcgacta cctccagcgc
     6961 atcacgcagc tttccaagcc gccgtcggtc aactcccagc ttgagaagtc tctgctcgcc
     7021 gaaagcagca ccggggcggt cgaggacgat ccgctgcgct tcgacctgcg tatcgacttc
     7081 ttcaagcagg acgaggggcg tgtggtgacg gccttcaccg tccagaccga gaaccgcgaa
     7141 ctgagcttca aggacgtggg cggcatccag acggcgcgtc tcaacatctt cggccgcgtg
     7201 atgtcggtgg cgggccgcaa ggtcgcctct tttgaagacc cggtgacgac ggtggcgacg
     7261 gtcgaggatt tggaagcgaa gcgcgaccag aagtcggcct accagcgtct ggtggcgctc
     7321 acgccgggca cctacaagat cgacatgatc gtgcgcgacg tgacttcggg caagcagcac
     7381 gtcgccagcc acggcttcac cgtgccgaag tacgacgcca agctgctcgc cacctcgacc
     7441 atcgtgctcg ccgcgcgcct gcacggcctc gaaggccagc cgcccgtcgg catgttcacc
     7501 atcggcaact acaaggtcat ccccaacgtc tcgggcacgt tcaagcgcgg tcaggaggtc
     7561 ggcatctacg cgcagattta caacgcgggc atcgaccaga cgacgctccg gccgtcggtc
     7621 gatgtggagt acgcgctcgt cagggacggc aaggacgttt accgccagac ggaagactgg
     7681 cagggactct ccgacgccgg ccagcgcctg acgctcgcgc gcctgctccc gaccgaccag
     7741 ctcacgcccg gcagctacga gatcgccatc cgcatccgcg accgcgtctc cggccagacg
     7801 ctcacgcccg tctccaagtt cacggtcacc gccgagaagt aggcgccggc cccttcgagc
     7861 ggaaacaact tcaggcggcg cgcccttccg gtcgagagcg cgccgcctga atctttttcc
     7921 tacctccgcg tgaactctgc gctcattgcg tctctgcggt gaatctatct ccctttcaca
     7981 caagtaaaca tcgctcaaaa aaacatgccg cccgctacgc cgcacccgca cgactctgtt
     8041 agcatgaccc ccgtcgcttt tcccgcttcg gcccgcagcc cttttcaaga ttttcaagac
     8101 ttccgaggtg tccgccatgc gccaacccgc gacccgcttc gccctcctgt cgttcgtcct
     8161 cctcgtcttc gcacttcccg cttacgcgca ggaagacctc gacgcccgcc tcaaggagat
     8221 cgacgagtat gcggccaaag ccggacgcga ctggaaggcg cccggcttca gtgtcgccat
     8281 cgtcaaggac gaccgggtgg tcttcgtcaa aggctacggc gtgcgcgagc ttggcaagcc
     8341 cgacgcggtg gacgcagaca ccatcttcgc catcggctcg aacacgaagg cgttcacgtc
     8401 ggccgcgctc gccgtgctcg tggacgaggg caaggttagt tgggacgacc ccgtcacgaa
     8461 gcacctgccg ggcttccagc tcttcgaccc ctacgcgacg cgtgagatga gcgtgcgcga
     8521 cctcctcagc caccgcagcg gcctcgccac cttcgccggc gacctgctct ggtacgaatc
     8581 ctcctacggc cgcgacgaga tcatccggcg cgcgcgccac ctgcggcccg cctcctcctt
     8641 tcgcagccgc ttcggttacc agaacatcat gttcctcgcc gccggagaga tcgtccccgc
     8701 cgtcgccggc aagtcgtggg acgactttct caaagagaaa ttcttcgacc cgctcggcat
     8761 gcgtcgaacg ctgacgcgcc acgccgattt cgcgcgcgcc tctaacgtcg ccgcgccgca
     8821 caacgaggtg gactcccgcc tgcgctccat cccccacggc aacgtggaca acgtgggcgg
     8881 ggccggggcg atcaagtcgt cggccgcgga catggcccaa tgggtgcgcc tccagctcgc
     8941 gcgcggcaca cgcgacggcc ggaagatttt cggcacggcc gcgtcgcgcg agatgtggac
     9001 gccgcacgcc gtcctcggcg cggtgagcga gagcgcggag aagtttaatc cgacgacaca
     9061 cttcggtctc tacggcctcg gctggattct gaacgactat cagggacgcc tcctcgtctc
     9121 acacggcggc ggcctcgacg gcatgatctc gcgcgtcgcg ctgatgcccg aggagcggct
     9181 cggcttagtc gtcctcacca acagcgagac ctcgctttcg tccgccctct ctaacaagat
     9241 tctcgacgtc ttcctcggcg tcgcccgccg cgactggagc gccgacttcc tcgcgcgcac
     9301 gaagcaagct aaggaaactt ccgaggccgc cgccaggaag gtcgaagacg cgcgcgtccc
     9361 caacacgaag ccgtcgcttt cgcttgagtc ttacgccggc gtctacacgg gcgagatgtt
     9421 cggcgacgcg cgggtcacgg ccgagggcgg ccgtctcgtc ctgcgcctgc ttccctcgcc
     9481 caagttcgtc ggcgatctgg agcactggca cttcgacacc ttccgcgtcc gctggcgcga
     9541 gggggtcgtc tatcccttcc cgcgcggctt cgtcaccttc accctcgacg cgcagggcaa
     9601 gcccgccgag ttgaagctcg acgtgcccaa cttcgacttc gacttcaagg aaatagaatt
     9661 gcgccgcgcg cccgacgcgc aaacgccgcg cagcaactaa cgctcggcgt cgcccaatga
     9721 gtcggcccat gcacaaagag ccacagcgcc gcatcaatga cacactcgcg cgcggcgtga
     9781 ggcttcgttg ccccgcctgc gggcgcgcgt ccatcttcga gacgccgttc cggcgcaggc
     9841 ccgcctgccc ctcttgccgc gtgtcgttcg agcgcgagga gggctacttc gtcggcgcca
     9901 tctcgatcaa cctcgtcacg acggaggcgg cgctcctcat cgtctacctc
//
LOCUS       DASVES010000367         5316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1126478, whole genome shotgun sequence.
ACCESSION   DASVES010000367 DASVES010000000
VERSION     DASVES010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126478"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..297)
                     /locus_tag="VF240_15090"
     CDS             complement(34..297)
                     /locus_tag="VF240_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176589.1"
                     /translation="MTEQRIGSYILEELIGRGGMGVVYKGRHAEMPRIVQPAQQGPPN
                     RPFVMWVREIQMFGARLLTLSVPMRLALTPTVPRAEASRVCLQ"
     gene            complement(449..1198)
                     /locus_tag="VF240_15095"
     CDS             complement(449..1198)
                     /locus_tag="VF240_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176590.1"
                     /translation="MVTKGQEAAQRILAIIVIMAMLSVMGIPLTVIIFFGTVVYFVWR
                     AVQRSEQQDVKNIFDFYISANEVLRDDEKRWYGFEIDAVIKEGEGVLQMMSDPPPLVH
                     FALGALYGRAGEDEASAEHLAYVVESEHGDEGRRLAASPELRRYVQVLRRLEREPAEA
                     PLTMAAIRTLERLRRTRAEGLLEESRARLQGAAHRLPAATNTQRETLGAGLTPVAGTR
                     SQPTLAPETRSVPPPISEVLRDVYEEEKKTA"
     gene            complement(1257..2327)
                     /locus_tag="VF240_15100"
     CDS             complement(1257..2327)
                     /locus_tag="VF240_15100"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX7176591.1"
                     /translation="MTKDSKRVAVVAGDGIGPEVTDEAVAVLERVRETHGVALELQHF
                     DWGADKFLKEGVSLPEGALEMLARDFDAIFSGAFGDPRVPSNQHAEDILLGMRRGLDL
                     YINLRPVRLLDARLTPLKGRETKDIDFVVFRENTEGAYCGAGGFLKRGTPDEIATQEE
                     LNTRRGVERIITAAFEYARSEGRGRVTMADKSNVQRFGGDLWQRVFREVAAQYSEIEA
                     KHQYVDAMAMFMVLDPSQYEVIVTNNLFGDILTDLGAALQGGLGLAASGNIHPGRVSL
                     FEPVHGSAPPLAGRNEANPAGAILSAALMLEYLGHGAAARSVERAVGAAIAEGATTRD
                     LGGSLSTTAAGAEVRRRIMGEA"
     gene            complement(2351..3367)
                     /locus_tag="VF240_15105"
     CDS             complement(2351..3367)
                     /locus_tag="VF240_15105"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7176592.1"
                     /translation="MSGGCVLLIAIAVLQLTPRARASVHSQTGGHGAAQQPAQDEATA
                     AFDEKRAYEHVRKLVEFGPRPSGSKQLEKSREYIVAELKKYGLKVTTDEFKVETPAGR
                     RKMVNVTAEVAGDSADFIILASHYDTKLFKNLRFVGANDGGSSTGVLLELARAISSTA
                     RKPRFGYRLVFFDGEEAVCREWDECSKPGAPDNTYGSRRYVAALQERDELKRLRAMIL
                     LDMVGYRRLQLGRDSMSTKWLVDVIWQAARERGYGEQFVEREEGIGGDDHEPFLKAGV
                     PAVDIIQLNSYPHWHTAEDTLDKIAPRSLKVVGDAVLSSLPRVEEALWKKDEGRMKTG
                     ASKQ"
     gene            complement(3391..3981)
                     /locus_tag="VF240_15110"
     CDS             complement(3391..3981)
                     /locus_tag="VF240_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7176593.1"
                     /translation="MSVNVEWKTEDGGRTGVVAEGAYLWDAAKRLGVRLPAECDGRGE
                     CDTCAVAVEEGATLLSALTDAERVRLSPERLLAGERLACQTKVERGGQLVLRPVPVTE
                     REPTADESVRDFRKDFAEMPLKRKLTTLVELEATAAFHTLSAVADFPFAAFNKGLEFM
                     AGFGRQMSRRERDARRPAEHRPAGAGPAPEAGQKKD"
     gene            complement(4149..4667)
                     /locus_tag="VF240_15115"
     CDS             complement(4149..4667)
                     /locus_tag="VF240_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX7176594.1"
                     /translation="MPEKFTEEKDIFHRHIQHAGLKRTAQRDLILEVFLRTEEHLSSE
                     DLYNLIKQEDPTVGHTTVYRTLKLLVEAGLAREVRFGDGRMRYEHNYKHPHHDHMICT
                     ECGTTIEFFSAELEAIQDAILAKHKFVPIQHSLRIFGVCEKCQSTTAARPERDESAAE
                     PRVRFANAPSTH"
     gene            complement(4911..>5316)
                     /locus_tag="VF240_15120"
     CDS             complement(4911..>5316)
                     /locus_tag="VF240_15120"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX7176595.1"
                     /translation="GVAPYGGNYHVLKRAIEHFNLDTSHFTGQLWNKGRTIGPKQPLQ
                     KYLSNELQISSYKLKSRLISEGLFAPVCSDCGQTEWLGKPIPLELDHIDGNRSDNQLS
                     NLRLLCPNCHAFTPTYRGRNKSSKLSSVLPSA"
BASE COUNT          877 a         1747 c         1702 g          990 t
ORIGIN      
        1 cctcgcgggt ctcgcgtgcg cgccgcgggc ccctcattgt aggcataccc gcgaggcttc
       61 cgctcgcggt actgttggag tgagcgccag gcgcatggga acactcagtg ttaggagccg
      121 agcgccgaac atctgtattt cgcgcaccca catgacgaag gggcgattgg gcggcccctg
      181 ctgcgctggc tggacgatgc gcggcatctc ggcgtgacgc cccttgtaga cgacgcccat
      241 gccgccgcgc ccgataagct cttcgaggat gtaactgccg atgcgctgct cggtcatccc
      301 tgcaccgtcg aactaggcca acagataatt gatgagcgtg agaatcagtt tgactgacga
      361 acttatatca gaaaaaacgc gcccgacaaa aagagaggcg cggcgaacct cggatggtcg
      421 ccgcgcctcg ctcctcgcgg gtgtccgctc aggccgtctt cttctcttcc tcgtaaacgt
      481 cgcgcaggac ttcggagatg ggtggaggca cgctccgggt ctcgggggcg agcgtgggct
      541 gagagcgcgt gcccgccacc ggggtcaggc ccgcgccgag cgtctccctc tgtgtgttcg
      601 ttgcggccgg gagtcggtgc gccgcccctt gcagccgcgc gcggctctct tcgagcagcc
      661 cctcggcgcg cgtgcggcgc aggcgctcaa gggtgcggat ggcggccatc gtcagaggcg
      721 cctcggccgg ctcgcgctcc agccggcgca acacctgcac gtagcgtcgc agctcgggcg
      781 aggccgcgag ccggcggccc tcatcgccgt gctcgctctc gacgacgtag gcgaggtgtt
      841 cggccgacgc ctcgtcctcg ccggcgcggc cgtagagcgc gccgagcgcg aagtggacga
      901 gcggcggggg gtcggacatc atttgcagca cgccctcgcc ctccttgatg acggcgtcga
      961 tctcgaagcc gtaccaacgt ttctcgtcgt cgcgcagcac ctcgttggcg ctgatgtaga
     1021 agtcgaagat gttcttaacg tcctgctgct cggaacgctg gacggcgcgc cagacgaagt
     1081 agacgaccgt gccgaagaag atgatgacgg tcagagggat gcccatcacg ctcaacatcg
     1141 ccatgatgac gatgatggcc agaatcctct gcgctgcttc ctgccccttc gtgaccataa
     1201 ggctcccccg cccttgtcct cgtcgaagct acaggccgcc tagtcgcgcc tcggcctcac
     1261 gcctcgccca taatacgccg ccgcacctcc gccccggccg cggtcgtcga gagcgagccg
     1321 ccgaggtcgc gcgtcgtcgc gccctccgca atcgccgccc cgaccgcccg ttcaaccgag
     1381 cgcgccgccg ccccgtggcc gaggtattct agcatcaacg ccgcgctcag aatagcacct
     1441 gccggattgg cttcgttccg gcccgcgagc gggggcgcgc tgccatgcac cggctcgaac
     1501 agcgacacgc ggccgggatg aatgttcccg gaagcggcca gccccaagcc cccctgcaac
     1561 gccgcgccga ggtcggtcag gatgtcgccg aacagattgt tcgtgacgat cacctcgtac
     1621 tgcgacgggt cgagcaccat gaacatcgcc atcgcgtcaa cgtactggtg cttcgcttct
     1681 atctcggaat actgggcggc gacttcgcga aagacgcgct gccagaggtc gccgccgaag
     1741 cgttggacgt tcgacttgtc ggccatcgtc acacgccccc gcccctccga gcgcgcatat
     1801 tcgaaggcgg ccgtgatgat gcgttcgacc ccgcgccgcg tgttcaactc ctcctgcgtg
     1861 gcaatctcgt cgggcgtccc ccgcttcagg aagccgccgg cgccgcagta agcgccctcg
     1921 gtgttttcgc ggaagacgac gaagtcgatg tccttagtct cacgtccctt gagcggcgtg
     1981 agacgcgcgt cgaggaggcg gacggggcgg aggttgatgt agaggtcgag gccgcggcgc
     2041 atgccgagca ggatgtcctc ggcgtgttgg ttcgagggca cgcgcgggtc gccgaaggcg
     2101 ccggagaaga tggcgtcgaa gtcgcgcgcg agcatctcca gcgcgccctc gggcagactc
     2161 acgccctctt tgagaaactt gtcggcgccc cagtcgaagt gttgcagctc cagcgcgacg
     2221 ccgtgcgtct cccgcacgcg ctcaagcacc gcgacggcct cgtctgtcac ttccggcccg
     2281 atgccgtcgc ccgccacaac cgccacccgc ttcgaatcct tcgtcacgcg tctctccgag
     2341 tcattggaat ctactgctta ctggctcccg tcttcattcg tccctcatcc ttcttccaca
     2401 atgcctcctc cacacgcggc aggctactga gcacggcgtc gccgacgacc ttcagactac
     2461 gtggggcgat tttgtcgagc gtgtcttcgg ccgtgtgcca gtgcgggtag ctgttcagtt
     2521 gaatgatgtc cacggcgggg acgccggcct tgaggaacgg ctcgtggtcg tcgccgccga
     2581 tgccctcctc gcgctcgacg aactgctcgc cgtagccgcg ctcgcgcgcc gcctgccaga
     2641 tcacgtccac gagccacttc gtgctcatcg agtcgcggcc gagttgcagc cgacgatagc
     2701 cgaccatgtc gaggaggatc atcgcgcgca ggcgcttcaa ctcgtcgcgc tcttgcagcg
     2761 cggcgacgta gcggcggctc ccgtaggtgt tgtccggcgc gcccggcttc gagcactcgt
     2821 cccactcgcg gcagacggcc tcttcgccgt cgaagaagac gagacggtaa ccgaagcgcg
     2881 gcttgcgcgc cgtggaagag atggcgcggg ccagctccag caggacgccc gtggaggagc
     2941 cgccgtcgtt ggcgccgacg aagcgcaggt tcttgaacag cttggtgtcg tagtggctgg
     3001 cgaggatgat gaagtcggcg ctgtcgcccg cgacctccgc cgtgacgttg accatcttgc
     3061 gccgcccggc gggcgtctcg accttaaact cgtcggtcgt aaccttcagg ccatacttct
     3121 taagctccgc gacgatgtac tcgcgcgact tctccaactg cttcgacccg gaaggccgcg
     3181 gcccgaactc gacgagtttt ctgacgtgct cgtaggcgcg cttttcgtcg aaagccgcgg
     3241 tggcctcatc ctgtgcaggt tgttgggccg cgccgtgccc gccggtctgt gagtggacgg
     3301 acgcgcgcgc gcgcggcgtg agttgcagga cggcgatggc gattagcagc acgcagcccc
     3361 cggacaataa tttctcgcac gccggcacgg tcagtccttc ttctggcccg cctcaggggc
     3421 cgggccggcc ccggcggggc ggtgctcggc ggggcggcgc gcatcgcgct cgcggcgcga
     3481 catctggcga ccgaagccgg ccatgaactc aagccccttg ttgaaggcgg cgaacgggaa
     3541 gtcggcgacc gccgagagcg tgtggaaggc ggccgtcgcc tcaagctcga cgagtgtggt
     3601 cagcttccgc ttgagcggca tctcggcgaa gtctttgcgg aagtccctga ccgactcgtc
     3661 ggccgtcggc tcgcgctccg tgacgggcac gggccggagc acgagctggc cgccgcgctc
     3721 gaccttcgtc tgacaggcga gacgctcgcc cgcgagcaaa cgctccgggc taagacgcac
     3781 gcgctcggcg tcagtcagcg ccgagaggag cgtggcgccc tcctcgacgg cgacggcgca
     3841 cgtgtcgcac tcgccgcgcc cgtcgcattc ggccggcaga cgcaccccca gccgcttcgc
     3901 cgcgtcccac agataagccc cttccgcgac gacgcccgtg cgccctccgt cttcagtctt
     3961 ccactcgacg ttaacactca ttagcagaca cctctacggt caaaatctcg cacacagttg
     4021 ttccggggag ccgtcttgaa agttacaccc acgacgcgaa gaaggcaaaa ggaaaaaggt
     4081 aaaaggcaaa agtgcaaggg cgggaatcct tttcactttt gccttttacc tttttccttt
     4141 tgccttcctc aatgtgtgct cggggcgttg gcgaagcgga cgcgcggctc ggcggccgac
     4201 tcgtcgcgct cggggcgggc ggccgtggtg ctctggcact tctcgcagac gccgaagata
     4261 cgcagggagt gctggatggg cacgaacttg tgcttggcga ggatggcgtc ctgaatggct
     4321 tccagctcgg ccgagaagaa ctcgatggtc gtcccgcact cggtgcagat catgtggtcg
     4381 tgatgcgggt gcttgtaatt gtgctcgtag cgcatgcgcc cgtcgccgaa gcgcacctcg
     4441 cgggccagcc ccgcttcgac gaggagcttg agcgtgcggt agacggtcgt gtggccgacg
     4501 gtcgggtctt cctgcttgat gaggttgtag aggtcttcgc tggacaggtg ctcctcggta
     4561 cgcaggaaga cttcgaggat gaggtcgcgc tgcgccgtgc gcttcagacc cgcatgctga
     4621 atgtgccggt gaaagatgtc tttctcttcg gtgaattttt caggcatagg cgcttccccg
     4681 cgactccgca aaccgtcaca ctaacttttc cgcgaaccca aactgtgcgc ccattctaac
     4741 acccgtctcg ccgcccgcgc gagtggtttg tgaggcccgc gcgccacatg ctatcttccc
     4801 acgaacacgg ccggggtggc gaaatggcag acgcagcgga cttatccgtt tgagcaccca
     4861 gagcgaaacc tccggcgtga atggggtcaa attcggtgaa cccttagcag tcaggctgat
     4921 ggcaacaccg agctaagttt ggaggatttg ttcctgccgc gataggtcgg ggtgaatgca
     4981 tgacagttgg gacaaagtag ccgcaagtta gagagttggt tatcggagcg attgccgtcg
     5041 atgtgatcca gctccagagg gattggtttt ccgagccatt ccgtctggcc acagtcggaa
     5101 cacaccggag caaacagccc ctcggaaatg agcctgcttt ttaacttgta agagcttatc
     5161 tgaagctcgt tgctcaagta cttttgcagc ggctgttttg ggccgattgt cctgccttta
     5221 ttccaaagtt gtccggtgaa gtgggaagtg tccaggttaa agtgctcaat agcccttttc
     5281 agaacgtggt aattcccacc gtaaggagcg acgcca
//
LOCUS       DASVES010000368         5903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1681033, whole genome shotgun sequence.
ACCESSION   DASVES010000368 DASVES010000000
VERSION     DASVES010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681033"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VF240_15125"
     CDS             <1..843
                     /locus_tag="VF240_15125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176596.1"
                     /translation="VVAIVVGVGIGALQGSLVAYANVPAFIVTLGGLLAWRGMLLRVS
                     RGETIPLQIPFFISLGRDLLAPSVGYALAALAVAAIVITNLRRNSARRRHGLALSGAG
                     ATAARIVLPSVVIVAFVAYLNTRGGVPIPVVVLLAVALVGAFLMRNTTFGRYLYAIGG
                     NPDAARLSGLSIRRHILAAFCVLGALAGVAAILHTARLQAATPDAGTLMELDAIAACV
                     IGGTSLMGGRGTVFGAILGALIMASLDNGMTILGVQNSTQYVIKGAVLVIAVWFDMLG
                     RRKG"
     gene            1003..4572
                     /locus_tag="VF240_15130"
     CDS             1003..4572
                     /locus_tag="VF240_15130"
                     /EC_number="3.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004339 - glucan 1,4-alpha-glucosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009251 - glucan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan 1,4-alpha-glucosidase"
                     /protein_id="HEX7176597.1"
                     /translation="MRKIFLHVLALCLLLSFTTTRSLAQARAGGDAPGAPGRDAHWES
                     AGKQGVGTSNTLESKVWFTLRGGVLTEIYYPTVDVAATQALQFVVVSGGNRRVETEAE
                     DATHRVEVADARSLTFRQINTAKSGAWTITKTYIADPRRHTVLVEVFFTSRPAGAHTL
                     YVYHDPSLANSGMHDAAWTQDGALLASEADKASALVSGVGFNEMTNGYLGTSDGLTQL
                     RRAGRLTPYARAADGNVVQVASLPRVTKSGAVSFTLALAFGKEPREALSNARASLAAG
                     FRSVRAEYETGWHEYLGRLRRVAPKVQAQFDMAAMVLKAHEDKTYRGAMIASLSVPWG
                     GGASANEPEVGGYHLVWSRDLYQVATAFHALGDKASAERALNYLFTVQQKADGSFPQN
                     SWLDGRPFWGSLQHDEVAYPLILAQQLGRTDRETYVKHVRPAADFIVRTGPWTPQERW
                     EEESGYSPSTIAAEIAGLVCAAEIARRHDDHASEAIYLAAADEWARNVEQWTATSTGK
                     HGDGNYYLRITGDGDPNDGSLLELNNGAGSFDEREIVDAGFLELVRLGIKPAADPLVA
                     KSLAVIDRRIKVETPGGASFYRYNHDGYGEMDDGRPWNFDGKYTGKGRLWALLAGERG
                     EYELARAQAARARLRLDAMLGFANEGRMIPEQVWDKPQTPRPDFSFGEGTGSATPLAW
                     SMAQFIRLAASLREGRNVALPEAVAARYARRATPPARADVSFNAPDERELKIAEAGKS
                     IKLAGRVARGGRAYLLFGGERRELKTDESGRYEFEFVAPAGESQVVVGTNAASGATSF
                     HRVSLRALTPEERRKAEGEQAPPRLLERIRNAKTSPLIDGDRVTFVYRGRARRVEVVG
                     DFTSWSPRSLVMREAPGSDVKYYTREFARDARAEYKLIADGEWMLDPLNSNRNDNGVG
                     GENSNFAMPGYRPSAYVVEPRDINPLRASSGGRMGEAGSNVPDISTPTFVLDEVPSKI
                     LGGSRKINVFLPRSYANSNEALPLPVLYVQDGSEYIRRAQAAIIADKLIEQKKVAPFI
                     IVFVDPADRMKDYWASDQFADFMARELVPFIDARFRTQPRREGRALIGASLGGVTSIW
                     AGLKHPEVFSRVGAQSSALQIDDERVVSALNRLAPAGGSGSLPLRFYMDVGRMEPVLD
                     AHRRVRVMLRAKGYPVTYRELEAGHNYTAWRDLLADALIALWK"
     gene            complement(4894..4965)
                     /locus_tag="VF240_15135"
     tRNA            complement(4894..4965)
                     /locus_tag="VF240_15135"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4931..4933),aa:Val,seq:cac)
     gene            complement(5080..5580)
                     /locus_tag="VF240_15140"
     CDS             complement(5080..5580)
                     /locus_tag="VF240_15140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7176598.1"
                     /translation="MKRQHNTALRARGFSLVELMIVIAIIGILVGVGVPAWRNAVIAG
                     NETAAIQNLRNIATEQRTYFNMNGRNGYATFDQLQIDPKFKGDAPVVEGFVYTLKIVP
                     KTQSSPPSFTLNADPQAKDGIGATGKRHFYIDSNVGNVRTNPNGPAGPNDSGDGSDAA
                     PAEAAK"
BASE COUNT          991 a         1975 c         1910 g         1027 t
ORIGIN      
        1 gtcgtggcca tcgtcgtcgg cgtcggcatc ggcgcgttgc aggggagcct cgtcgcctac
       61 gcgaacgtcc cggctttcat cgtcacgctc ggcggcctgc tcgcgtggcg cgggatgctc
      121 ctgcgtgtca gtcgcgggga aaccatcccg ctccagattc cgttcttcat ctctctcggt
      181 cgcgacttgc tggccccgtc cgtcggctac gcgctcgccg cgctggccgt cgcggccatc
      241 gtcatcacaa acctgcgacg caactccgcg cgccgccgcc acggcctcgc actctcgggc
      301 gccggggcga cggccgcgcg catcgtgctg ccgtcggtcg tcatcgtcgc cttcgtcgcc
      361 tatctgaaca cgcgcggggg cgtgcccatc cccgtcgtcg tcctgctggc cgtggcgctc
      421 gtcggcgcat tcctgatgcg taacacgact ttcggccgct acctctacgc catcggcggc
      481 aaccccgacg ccgcgcgcct ctccggtctc agcatccgcc ggcacatcct cgcggccttc
      541 tgcgtgctgg gcgcgctcgc cggcgtggcc gccatcctcc acaccgcgcg ccttcaggcc
      601 gccacgcccg acgccgggac gctgatggaa ctcgacgcca tcgccgcctg cgtcatcggc
      661 ggcacctcac tgatgggcgg gcgcgggacg gtcttcggcg ccatcctcgg cgcgctcatc
      721 atggcgagcc tcgacaacgg catgaccatc ctcggcgtcc agaactcgac gcagtacgtc
      781 atcaagggcg cggtgctcgt catcgccgtc tggttcgaca tgttgggccg gcgcaaaggc
      841 taagagcgcc ttctgaagaa acaattaaca tggatggaca ggatgggcag gatgaagaaa
      901 aagctgattt gagatttcag attcgagatt tgagatttca gatttcaaat cttctcatcc
      961 tatccgtcct gtccatccat gttaatttct tccgcgactc agatgagaaa aatcttcctc
     1021 cacgtccttg ccctctgcct cctcctgtct ttcacgacga cgcgctcact cgcgcaggct
     1081 cgcgccggcg gcgacgcgcc cggcgcaccc ggccgggacg cgcattggga gagcgccggc
     1141 aagcagggcg tcggcacctc gaacacactc gaatcgaagg tctggttcac gctgcgcggg
     1201 ggcgtgttga cggaaattta ttatccgact gttgacgtgg ccgccacgca ggcgttgcag
     1261 ttcgtcgtcg tcagtggcgg caaccgccgc gtcgagaccg aggccgaaga cgccacgcat
     1321 cgcgtcgagg tggcggacgc gcgctcgctt accttccggc agatcaatac ggcgaagagc
     1381 ggcgcgtgga cgatcacgaa gacctacatc gccgacccgc gacgccacac cgtgctcgtc
     1441 gaagtcttct tcacgtcgcg ccccgccggg gcgcacacgc tctacgtcta tcacgacccc
     1501 tcgctcgcca acagcgggat gcacgacgcg gcgtggacgc aggacggtgc gctgctcgcg
     1561 tcggaggctg acaaggcttc ggcgctcgtc tccggcgtcg gcttcaacga aatgacgaac
     1621 ggctacctcg ggacgagcga cggcctgacg caactgcgtc gcgccggacg gctcacgcca
     1681 tacgcgcgcg cggccgacgg caacgtcgtg caggtggcga gcctgccgcg cgtgacgaag
     1741 tcgggggccg tctcctttac tctcgcgctc gccttcggaa aggagccgcg cgaggcgcta
     1801 tcgaatgcgc gcgcgtcttt ggcggcgggg ttccgcagcg ttcgcgccga atacgagacg
     1861 ggctggcatg agtaccttgg caggctgcgg cgtgtcgcgc cgaaagttca ggcgcagttc
     1921 gacatggcgg caatggtttt gaaagcgcac gaggacaaga cgtatcgcgg ggcgatgatc
     1981 gcgtcgctgt cggtgccgtg gggcgggggc gcgagcgcca acgagccgga ggtcggcggc
     2041 tatcatctcg tctggtcgcg cgacctctat caagtggcca cggccttcca cgcgctcggc
     2101 gacaaggcat cggccgagcg cgcgctgaac tacctcttca ccgtccagca gaaggcggac
     2161 ggcagttttc ctcaaaactc atggctcgac ggccgcccct tctggggctc gctccagcac
     2221 gatgaggtcg cctacccgct catcctcgcc caacaactcg gacgcaccga ccgcgagact
     2281 tacgtcaaac acgtccgccc cgccgccgac ttcatcgtcc gcacggggcc gtggacgccg
     2341 caggagcgtt gggaggaaga atccggctac tcgccctcga ccatcgcggc cgagatcgcc
     2401 ggactcgtgt gcgcggccga aatcgcgcgc cgtcacgacg accacgcttc cgaggccatc
     2461 tacctcgccg cggccgacga gtgggcgcgc aacgtcgagc agtggacggc gacctcgacc
     2521 ggcaagcacg gcgacggcaa ctactacctc cgtatcacgg gcgacggcga cccgaacgac
     2581 ggctcgctgc tcgaactcaa caacggcgcc ggctccttcg atgagcgcga gatagttgac
     2641 gccggctttc tcgaactcgt ccgtctcggc atcaagccgg ccgccgaccc gctcgtcgcc
     2701 aagtcgcttg ccgtcatcga ccgccgcatc aaagttgaaa caccgggcgg cgcttctttc
     2761 taccgctaca accacgacgg ctacggcgag atggacgacg ggcggccgtg gaacttcgac
     2821 ggcaagtaca cgggcaaggg gagactgtgg gcgctcctcg ccggcgagcg cggcgagtat
     2881 gaactcgcgc gcgcacaggc cgcgcgtgcg cgcctgcgtc tcgacgcaat gctcgggttc
     2941 gccaacgagg gacgaatgat cccggaacag gtctgggaca agccacagac gccgcgcccg
     3001 gactttagtt tcggggaggg aaccggctca gccaccccgc tcgcgtggtc gatggcgcag
     3061 ttcatccggc tcgccgcgag cctgcgggag gggcgcaacg tcgcactccc cgaggcggtc
     3121 gccgcgcgct acgcgaggcg tgcgacgccg cccgcccgcg ccgatgtctc cttcaacgcg
     3181 cccgacgaac gggagttgaa aattgcggaa gcgggaaaga gtatcaagtt agcggggcgg
     3241 gtcgcgcggg gcgggcgcgc gtacttactc ttcgggggcg agcggcgcga gttgaagacg
     3301 gatgagagtg gtcgttacga attcgagttc gtcgcgccgg ccggcgagtc gcaagtcgtc
     3361 gtcgggacga acgcggcttc gggggcgacg agttttcacc gcgtctcgct gcgcgctctg
     3421 acgccggagg agaggcggaa ggcggaggga gagcaagcgc cgccgcgttt gcttgagcgc
     3481 atcaggaacg ccaaaacgtc tccgctcatt gacggcgacc gcgtcacctt tgtctatcgc
     3541 ggtcgggcgc ggcgcgtgga ggtcgtcggg gatttcacga gctggagtcc gcgttctctg
     3601 gtgatgcgcg aggcgccggg gtcggacgtg aaatactaca cgcgcgagtt cgcgcgcgat
     3661 gcccgcgccg agtacaagct catcgccgac ggcgaatgga tgctcgaccc gctcaactcg
     3721 aatcgaaacg acaacggagt cggcggcgag aactccaact tcgcgatgcc gggctatcgg
     3781 ccctcggctt atgtcgtcga gccgagggac atcaatccgt tgcgcgcatc gtcaggcggg
     3841 cgaatgggtg aagccggctc caacgtcccc gacatctcaa cacccacttt cgtgctggat
     3901 gaggtgccca gcaaaatcct cggcgggagc cgcaagatca acgtcttcct cccgcgctcc
     3961 tacgcgaaca gcaacgaggc tttgccgctt cccgtgctct acgtgcagga cggctccgag
     4021 tacatccggc gcgcgcaggc cgccatcatc gccgacaagt tgatcgagca gaaaaaagtc
     4081 gctccgttca tcatcgtctt cgtcgatccg gctgaccgca tgaaggacta ctgggcgagc
     4141 gaccagttcg ccgacttcat ggcgcgggaa ctcgtcccct tcatcgacgc ccgcttccgc
     4201 acgcagcccc ggcgtgaggg acgcgccctt atcggcgcga gcctcggcgg cgtcacctcc
     4261 atctgggccg gtctcaagca cccggaagtg ttctcgcgcg tcggcgcgca gtcgtccgcc
     4321 ctccaaatcg acgacgagcg ggtcgtctcg gcgctcaaca ggctcgcgcc ggccggcggc
     4381 tccgggagtc tgccgctcag gttctacatg gacgtgggcc gcatggagcc ggtgctcgac
     4441 gcgcaccgcc gcgtccgcgt catgctccgc gcgaagggct accctgtcac ataccgcgaa
     4501 ctcgaagccg gccacaacta cacggcctgg cgcgacctgc tcgccgacgc gcttatcgcc
     4561 ctgtggaagt aaaaaggcaa aaggcaaaag ttgggaagcc gagccacgga cgccaacgag
     4621 ccgccggagg cggcgaaaga gtttagccca cggcgtgagc cgtggggacg agttcaatta
     4681 aagaatcgaa gccccggagg ggcgaaagaa tctcgaagtt gagtacgagt ctcgtcgaga
     4741 ttctttcgcc cctccggggc ttcaggttat ttctaaacgc tgttccccgg tctcacgacc
     4801 ggggctacaa tcttccgccg ctccgcggct cgttgaggcc cgcaacgacg cttgtgactc
     4861 ggcccctcac aattatcttc tgccttactc tgatgcccgc tgtaggactt gaacctacga
     4921 ccccacgcgt gtgaagcgtg tgctctacca ctgagctaag cgggcggtcg ttcggcggtg
     4981 tgcgaccggc gtggacgcgg gatgctaaca aagcgcaagc ggcggcgtca agaaactcgg
     5041 ccggaggcgt gcgctcggca aacttcaaag gccgcgttgc tacttcgcgg cctcggccgg
     5101 ggccgcgtcg ctgccgtcgc cggagtcgtt cgggccggcg gggccattgg ggttggtgcg
     5161 gacgttgccc acgttcgagt cgatgtagaa gtggcgcttc cccgtagccc cgatgccgtc
     5221 cttcgcctgc gggtcggcgt tgagggtgaa ggagggcggg ctgctctgcg tcttcgggac
     5281 gatcttcagg gtgtagacga agccctcgac gacgggcgcg tcgcccttga atttcgggtc
     5341 gatctggagc tggtcgaaag tggcgtagcc gttgcgcccg ttcatgttga agtaggtgcg
     5401 ctgctcggtg gcgatgttgc gaagattctg gatcgccgcc gtctcgttgc cggcgatgac
     5461 ggcgttgcgc caggccggca cgccgacgcc gacgaggatg ccgatgatcg cgatcacgat
     5521 catcagctcg accagtgaga agccgcgcgc gcgcagggcc gtgttgtgct gtcgtttcat
     5581 cttgccagta cctcgccggc ccatctacac gagccggccg cgtttaagtt ccaaaataag
     5641 ggcgactctc tgaacagcca cgctacgggg ctgctctcgg attggaaatt ttactacacg
     5701 gatgcggcgg aagccaccgg gcggggcgga agcctttggg aaggggtaag ggttaagggg
     5761 gaaaggtaaa agacggacag ggtcgccgta ccctcacggt tgttcgtctt cttcgagcgc
     5821 gccgacggct tcgcccttac cccttacccc ttaccctttc cccctttttc ccctgccacc
     5881 ttttgccttt cccccttcca ctt
//
LOCUS       DASVES010000369        15834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_454836, whole genome shotgun sequence.
ACCESSION   DASVES010000369 DASVES010000000
VERSION     DASVES010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15834
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454836"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..263)
                     /locus_tag="VF240_15145"
     CDS             complement(33..263)
                     /locus_tag="VF240_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176599.1"
                     /translation="MSTETITIPVDAEAAEAYRGASAEQRRKIQLLLGLWLKEAISAD
                     PAALKELMTDIGGKAQARGLTSEELKSILEEE"
     gene            complement(317..1297)
                     /gene="queG"
                     /locus_tag="VF240_15150"
     CDS             complement(317..1297)
                     /gene="queG"
                     /locus_tag="VF240_15150"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX7176600.1"
                     /translation="MSETIHDDAGRARSLSAKIRERARALGFEKVGIVRADALNEEAG
                     RLAQWLARGLHGEMAWMARAPERRTNPRELLPGARSVVAVAMNYYTPHEHAARPATGK
                     ISRYAWGDDYHDVLGERLRALLAWIEEECPGAEGRVCVDAQPAMDKAWAVRAGLGWIG
                     KHTNLITREYGSWVFLGELLLDLELEYDTARVEDHCGTCTLCIDSCPTGAIVEPYVVD
                     SNLCISHATIELRAPAEIPAAVAQRADGWLYGCDACQDVCPWNRFEQPTPEPRFEPRP
                     GNVSADLREILELTPDTYAARFRHSPIKRAKLSGLQRNARALLERRNKAE"
     gene            1467..2771
                     /locus_tag="VF240_15155"
     CDS             1467..2771
                     /locus_tag="VF240_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7176601.1"
                     /translation="MATNEQAGAGAETVEQKCGRLLFIGLAVTRLDKPTREMLKRVRP
                     GGVILFGRNVESPAQVALLNAQIRDCLAGRVLIGIDQEGGLVDRFRNICEPAPSAKAV
                     RNAGRADLAERFGALTARALRLLGFNINFAPVLDLGGDNDDNGLRGRTFGNDPGTVSR
                     LAGAYLDGLQRNHIVGCGKHFPGLGGSTVDSHRRLPVVSHTWEQIFERDLVPFMDLMT
                     HRPGERLHSVMVSHAAFPDMSEFVQAWFRRAGDPKTLTDLHRFPATISGNVVMRLLRQ
                     ALRYDGLVITDDMEMGAVVQTLSVAEASLSAIEAGSDMVLICEQEANFDAARNRLVEA
                     VGEGRLSERAVSAAVRRIDRLLKMAGEPEQFDSDEFAEVSRETGVLKQLLKSAEDTGE
                     YAPLFGTEEGDLRRSTNFGADDPVGNVGDIAEQIDAESKLIN"
     gene            3042..4442
                     /locus_tag="VF240_15160"
     CDS             3042..4442
                     /locus_tag="VF240_15160"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX7176602.1"
                     /translation="MRRTLAPILILSLLSSSFAQQQPQPQPSAPTPAPRVRAADYQDE
                     AARIIGAALTDDTAYRRLAYLGDRIGHRLSGSTQLEEAIRWAVSEMKRDGLDVRAEKV
                     MVPRWVRGAESVELIEPVRRSLVMLGLGNSVGTPPEGVTAEVVVVKSFDELDALGERV
                     KGKIVLYNVPFTNYGATVRYRGAGASRAARWGAVASLVRSVGPASLQTPHTGALGYDD
                     AQPKIPAAALTTEGAEMLQRMQERGERILLRLRMEAKFLPDSESANVVADLKGRERPD
                     EVVLVSGHFDSWDVGQGAHDDGGGCMMAWEAVRLLKKLGLRPRRTVRVVLYTNEENGL
                     RGGQGYLDAHRAEVAKHVLAIESDSGTFRPTGFGLGEGASVQARADITEIAKLLAAIK
                     ADRIGANGGGADIGPLMREGVPGMGLNVDGPYFNYHHTPADTVDKIDPQDMALCVAAM
                     AVMAYVVADMPASLNK"
     gene            complement(4716..7481)
                     /locus_tag="VF240_15165"
     CDS             complement(4716..7481)
                     /locus_tag="VF240_15165"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7176603.1"
                     /translation="MFQKKTLRLLVAALVVASQMLAVAAQDSAVPKIQFEKYSLPNGL
                     QVILHVDRKLPVVHVNQWFHVGSKNEKVNRSGFAHLFEHMMFQGSKNAPGEYFSYVEK
                     AGANLFTGGVNGTTNEDRTNYFATVPSSNLEYLLWLEADRIATLTDVLDKTKLDNQRD
                     VVKNERRQGLENVPYGRWDTLVTENIFPARHPYSWTVIGSHEDLTAATADDVKDFFKT
                     YYTPNNLSLVVAGDFDPAEAKRLVEKYFGPLPPGPSLDRPTRNVTTLVGEKIIEVNDR
                     VPQERVYMNWVTAPRFEPGDGELDIISTILTDGLSSRLNKTLVYDKQVASDVSAFQQS
                     REIASIYTIIATARPGASLPQIEQTITDEIARLAKEGPTPAELNRAKVKLESQYISGL
                     ERIGGFGGKSDLLNLYNTYLGDPNKFDFDLGRYRNATAEGVRDTAARWLNTRNRLLVR
                     FHPETSQLPQKADLDRSKQPAMGADKAYSAPEVKTAKLENGLEIFVVERKELPKVAVA
                     LSTRVGTAADPADKAGLVQMAYATMDMGTKTKNAIQIQDALGDLGTAIFAGPSRESST
                     TGFEVLKRNLNPAMTLFADVVLNPTYPASEIEREKKITLDNITQQMNDPNGIANRVRN
                     MVVFGADHPYGRPGLGFTSTVARITPEDLARFHQTYLKPSNSALFFAGDITVAEATAL
                     ARQHFGSWTGAAPPAQNIAAPQFASGGKVYLINKPDAAQTVVLQVLGAPQRKSDEFYA
                     FNLADAVWGGGFQSRLNLNLREQKGYSYGTFSNPVQYDRGSIWVAAGGVQTDKTKESL
                     VEFVSELKAIAGGKPITAEELADAKGNRVRGYAQQFESLGQLVGQVAQLWANGLPLTE
                     LQRLPDETQRATLAAVNAAAVKYAVPGKSTLILVGDLSKIEAGVRELNLGEIVILDAE
                     GRPVTQR"
     gene            7795..9816
                     /locus_tag="VF240_15170"
     CDS             7795..9816
                     /locus_tag="VF240_15170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7176604.1"
                     /translation="MPNELQREEPPDHETAHRRGADLHGPELAPYWLLAIIESADDAI
                     ISKTLESIITSWNKGAERIFGYTAQEAVGKPVTMLIPADHLDEEPAILARIRAGDRIE
                     HYETVRVRKDGTPIDISLTVSPIRNAAGQIIGASKIARDISTRRQSELAQAQLANIIE
                     SADDAIISKSLDSIITGWNKGAERIFGYTAEEIIGKSVTILIPADHPDEEPAIIARLR
                     RGERIEHYETVRIRKDGTPIDISLTVSPIRNAAGQIIGASKIARDITGLKRAERERER
                     LHKSEREARERAEELSHLKDEFLATVSHELRTPLTAILGWAHMLRTGQSDGDSNLKAF
                     EAIERNARSQAQLIEDLLDVSRIITGKLRIDVRPLDPGSFIDSAIEAVRPAAEAKGVR
                     VQKIMDTGAVSVAGDPVRLQQVFWNLLTNAIKFTPRGGRVQIRLERVNSHIEIAVADT
                     GAGITPEFLPHVFDRFRQADQKTTRRYGGLGLGLSIVRHLVELHGGTVRAESAGEGQG
                     STFTVLLPVAPVYQTGVEGGRVHPAARDTLPSYECADRLDGLRLLVVDDEQDTRELLK
                     AGLGRYGAEVTAAGSAAEALEAVRAAVPDLLISDIGMPGEDGYDLIRRVRALSAEAGG
                     RVPAIALTAYARVEDRMQALRAGYQMHVPKPVEMAELVAVAASLVRRGK"
     gene            complement(9891..11000)
                     /locus_tag="VF240_15175"
     CDS             complement(9891..11000)
                     /locus_tag="VF240_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176605.1"
                     /translation="MRNKIASTSVFTLLASAVFALLLSAGSSAASQEQPTIAKDSIQM
                     TPYTFNVYRGDYDKWSWVPRIAFRVNGPIPSGGQLYVEYTIPGAPAVKFDCPTEETEK
                     GRWWKTECGGRGIPEDKGSLYTGPVSFAIKLRNELAGADTTIFNGKTKVEKSLSSEHG
                     PKAAGKFVYFVNHDWNLPIGYVYLVPNSLKGWERPDFHVSFWVRGDAYNFKPHLFHNG
                     KEVGKKSLEGQEVGRAGCESDVENSTTQSVADTVPQKAKWARVRCSFPNIVGWDKSGD
                     NYDLFGPPHSLGASPGEYELKILWNNKLARSIKFTVGTDGKFDNGIASANKLGSDRVI
                     VPVQIIGDQDGAWNKMAWKTDAFYGSPLTGFTAAP"
     gene            complement(11230..12006)
                     /locus_tag="VF240_15180"
     CDS             complement(11230..12006)
                     /locus_tag="VF240_15180"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7176606.1"
                     /translation="MSKTRIVFIQRTARRRAFSFRLIAAGVALLCLVAAPVAAQTFRG
                     TILGTVTDPQGAVVQGAAVTARNTGTGLERTATTDADGNYTIAELPIGTYDVVVRATG
                     FAEGRVVGATVEVAGERRADVSLLVSGSAENVVVNATTTQVESTSNTLGGTIPTRQVE
                     DLPINGRDFTKFLVLVPGATGDPSGATDSPGSFGLFSANGNRGRSINYLLDGTDMNDG
                     YRNLPAINEAGVFGTPATILPVASFACTACAADARRREHG"
     gene            12306..13727
                     /locus_tag="VF240_15185"
     CDS             12306..13727
                     /locus_tag="VF240_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7176607.1"
                     /translation="MLIDDNNRKLIAQLRRTMNELAAPLGQSIRVVWQGGGAPDARAV
                     LDNQLLLTAQHFIDLREASDEEKQHYYGQVCAVFGLRAEDNTFPLSRSRDLPPHLLRR
                     LDRTPACVSYLDTFDARHGTAHADRARRCLFQFANLVIKSDGRITPAEELALVEFKHA
                     LYPEGAPASAVGGAGATAEAEAAPEEELPPPRPLEELLAELDALVGLERVKADVRQIA
                     NFIRVQKMREEKKLKTLPVSRHLVFYGNPGTGKTTVARLLSEIYRSLGVVRRGHLVET
                     DRAGLVAGYVGQTAIKTKEAVARSRGGMLFIDEAYTLSSGGAGDFGREAVETLLKLME
                     DHRDDLVVVVAGYTGKMQEFLDSNPGLRSRFNRHLRFDDYDTAQLVKIFQTFCKRADF
                     RITPEAEAQLAARFDVLCLMRDETFGNARTARNIFEVAISKQANRVVALTDITADALS
                     CIEPADIPTEEELRACGIITPER"
     gene            13944..15005
                     /locus_tag="VF240_15190"
     CDS             13944..15005
                     /locus_tag="VF240_15190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176608.1"
                     /translation="MSRVTRVALWLILGLSLSAAWAPAARARSSAVNTTPHVALQGRN
                     RISGHVFAEARRPVPDIYVELMDEVYTTIARARTNGSGFYSFEGLSTGNFKLKVLPYG
                     TDFEEQTRDITIYNISVVSGAGASSEQVDFYLKTRGSLVTSPFAAPGTVFAQDVPEEA
                     RKHYERGVALLGEKKDKEAFDSLKRSLEIFPRYYMALDRLGTEYVLRGYKTAAYILLT
                     EAVKVNPRGFSSLFGLGMSEFQLGMMNEAAESFRRATLSYDGSINAHMYLGLALKRTG
                     KLAQAEASLKRARDVGKGQTPEVHWHLARLYGEQKRYAEAADSLELYLKTQPQNPDPQ
                     KVAKIQQVIRQLREKATKG"
     gene            complement(15193..>15834)
                     /locus_tag="VF240_15195"
     CDS             complement(15193..>15834)
                     /locus_tag="VF240_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7176609.1"
                     /translation="GYVILGVNNRGSSGYGKTFFAADDGKHGREPLWDCIEAKKYLAS
                     LGYVDESKIGIIGGSYGGYMVLAALAFKPAEFAVGVDIFGVSNWVRTLQSIPPYWESF
                     RKALYKEMGDPATDLENLRAISPLFHADKIRRPLIVLQGKNDPRVILPESDEIVEAVK
                     KNNVPVEYVVFDNEGHGFTKKVNEIRANKAILEFLDKHLKGATGASESTSRAN"
BASE COUNT         2654 a         5156 c         5271 g         2753 t
ORIGIN      
        1 cgatgacgtt cgtatcaaaa acgaggcgca agttattcct cttcgagaat cgactttagc
       61 tcctcgctcg tcaggcccct cgcctgagct ttgccgccga tgtcggtcat caactccttg
      121 agtgcggcgg ggtcggcgga gatggcctcc ttgagccaga ggccgagcag cagttgaatc
      181 ttccgccgct gctcggcgga ggccccgcgg taagcctcgg cggcctccgc atcaaccggg
      241 atggtgatcg tctctgttga catcttggct tccccccttc agacacgcct tcgtaatcgt
      301 aagtcttaca gagagcctac tccgccttgt ttcgcctctc aagcagcgcg cgtgcgttgc
      361 gctggagacc ggaaagcttg gcgcgcttga tggggctgtg gcggaagcgc gcggcgtagg
      421 tgtcgggcgt gagttcgaga atctcgcgga ggtcggccga gacgttgccc ggccgaggct
      481 cgaagcgcgg ctcgggggtg ggctgctcga agcggttcca ggggcagacg tcctggcagg
      541 cgtcgcagcc gtagagccag ccgtcggcgc gctgggccac ggcggcgggg atttcggcgg
      601 gcgcgcgcag ctcgatggtc gcgtgggaga tgcagaggtt ggaatcgacg acgtaaggct
      661 cgacgatggc gcccgttggg cacgagtcga tgcagagcgt gcacgtgccg cagtggtctt
      721 cgacgcgcgc ggtgtcgtat tcgagttcga ggtcgagcag gagttctccg aggaagaccc
      781 acgagccgta ctcgcgcgtg atgaggttcg tgtgcttgcc gatccacccg aggccggcgc
      841 gcacggccca cgccttgtcc atcgccggct gcgcgtccac gcagacgcgc ccttccgcgc
      901 cggggcattc ttcttcgatc cacgcgagga gcgcgcgcag gcgctcgccc agaacgtcgt
      961 gatagtcgtc gccccatgcg tagcgcgaga ttttgcccgt ggccggacgg gcggcgtgct
     1021 cgtgcggcgt gtagtagttc atcgcgacgg cgacgaccga gcgcgcgccg ggcaaaagct
     1081 ctcgcggatt cgtgcgacgc tccggcgcgc gcgccatcca cgccatctca ccgtgaagtc
     1141 cgcgcgcgag ccactgcgcg agccggccgg cttcctcgtt gagcgcgtcg gcgcgcacga
     1201 tgccgacctt ctcgaatccg agcgcgcgcg cacgctccct gatcttcgcc gagagggagc
     1261 gggcgcggcc cgcgtcgtca tgtatggtct cgctcatgtc tcgctgtgaa gtataacaaa
     1321 gcggcgcgcg cctcgcgctc gccgcacgcc cctcgcacgc ctcgccccgg cggtgctaca
     1381 attcacgcat tctcccgatc aagtttgcag acacacatga gaggccggcg cgcgcgcgct
     1441 caggccgggc gcgggtcgag atattgatgg cgacgaacga acaggcgggc gcgggcgcgg
     1501 agacggtcga gcagaagtgt ggccgcctgc tcttcatcgg actggcggtg acgcggctcg
     1561 acaagccgac gcgcgagatg ctcaagcgcg tccgaccggg cggcgtcatc ctcttcggcc
     1621 gcaacgtcga gtcgccggcg caggtcgcgc tcctcaacgc gcagatccgc gactgcctcg
     1681 cgggccgcgt cctcatcggc atcgatcagg agggcggcct cgttgaccgc ttccgaaaca
     1741 tctgcgagcc ggcgccctcc gcgaaggccg tgcgtaacgc cggccgcgcc gatcttgccg
     1801 aacgcttcgg cgcgctcacc gcccgcgccc tccggctgct cggcttcaac atcaacttcg
     1861 cccccgtgct cgatctcggc ggcgacaacg acgacaacgg actgcgcgga cgcaccttcg
     1921 gcaacgaccc cggcaccgtc tcgcgtctcg ccggcgcata cctcgacggc ctccagcgca
     1981 atcacatcgt cggctgcggc aaacacttcc ccggcctcgg cggctcgacc gtcgattcgc
     2041 accgtcgcct gcctgtcgtc tcgcacacgt gggagcagat tttcgaacgc gacctcgtgc
     2101 ccttcatgga tttgatgacc caccgccccg gcgagcgtct tcattccgtg atggtcagcc
     2161 acgccgcctt ccccgacatg tccgagttcg tgcaggcgtg gttccgccgc gcgggcgacc
     2221 ccaagacgct gaccgacctc caccgcttcc ccgccaccat cagcggcaac gtcgtcatgc
     2281 gcttgctccg tcaggcgctg cgctacgacg gcctcgtcat caccgacgac atggagatgg
     2341 gcgctgtcgt ccagacgctc tcggtcgccg aggccagcct tagtgccatc gaggccggct
     2401 ccgacatggt gctcatctgc gagcaggagg ctaacttcga cgccgcgcgc aaccgtctcg
     2461 tcgaggccgt cggcgagggg cgtctttcgg agcgcgccgt ctcggccgcc gtccggcgca
     2521 tcgaccgcct gctcaagatg gccggcgagc cggagcagtt cgactcggac gaattcgccg
     2581 aagtctcacg cgagaccggc gtgctcaagc agttgctcaa gtcggccgag gacaccggcg
     2641 aatacgctcc gctcttcgga accgaggagg gcgacctgcg ccgctcgacg aacttcgggg
     2701 ccgacgaccc cgtgggcaac gtcggcgaca tcgccgagca gatcgacgcg gagtcgaaac
     2761 tcattaactg accacgcagc cccggcaggg gcggaagaat gtagcccacg gcgcgcgtcc
     2821 gtggggacag ccgcgttaaa aatttcgtag ccccggaggg gcgaaagaac ctcaagcgac
     2881 gtcacgcgaa ggcttgaggt tctttcgccc ctccggggct acgcatggcc tcgtcctttc
     2941 agttcccacg gacgcgagcc gtgggctaca tctggccgcg cctccggcgc tccttgattt
     3001 attcgcacgg acacccaaca ctctaacgga ggtcaaacgc cttgcgccgc acactagcgc
     3061 cgatcttaat tctctctctt ctcagttcgt cattcgcgca acaacagccg caaccgcagc
     3121 cttccgcgcc gacgcccgcc ccgcgcgtgc gcgccgccga ctaccaggac gaggccgcgc
     3181 gcatcatcgg cgccgcgctc accgacgaca ccgcctaccg ccgcctcgcc tatctcggcg
     3241 accgcatcgg ccaccgcctg agcggctcta ctcaactcga agaggcgatt cgctgggccg
     3301 tctcggagat gaagcgcgac ggtctcgacg tgcgcgccga gaaggtgatg gtgccgcgct
     3361 gggtgcgcgg cgcggagagc gtcgagttga tcgagccggt gaggcgcagt ctcgtcatgc
     3421 tcggtctggg caacagcgtc ggcactccgc ccgagggcgt gacggcggag gtcgtcgtcg
     3481 tcaaaagctt cgacgaactc gacgcgctcg gcgaacgtgt gaaaggcaag atcgttcttt
     3541 acaacgtgcc gttcacgaac tacggcgcga cggtgcgcta ccgcggcgcg ggcgcttcgc
     3601 gcgcggcacg ctggggcgcc gtcgcctcgc tcgtccgctc cgtcggcccc gcgagcctcc
     3661 agacgccgca caccggcgcg ctcggctacg acgacgcgca gcctaaaatc ccggccgccg
     3721 ccctgaccac cgagggcgcc gagatgcttc agcgcatgca ggagcgcggc gagcgcatcc
     3781 tgctgcggct caggatggag gcgaagtttt tgcctgactc ggagtcggcc aacgtcgtcg
     3841 ccgacctcaa ggggcgcgag cggcccgacg aggtcgtgct cgtcagcggt cacttcgact
     3901 cgtgggacgt gggtcagggc gcgcacgacg acggcggcgg ctgcatgatg gcgtgggagg
     3961 cggtgcgcct gttgaagaag ctcggcctgc ggccacggcg cacggtgcgc gtcgtgctct
     4021 acacgaacga ggagaacggc ctgcgcggcg ggcagggcta tctcgatgca caccgcgccg
     4081 aagttgcgaa gcacgtcctc gccatcgaga gtgattcggg caccttccgt cccaccggct
     4141 tcggtctcgg cgagggcgca tcggtgcaag cgcgcgccga catcacggag atcgccaaac
     4201 tcctggccgc catcaaggcc gaccgcatcg gcgcgaacgg cggcggcgcc gacatcggcc
     4261 cgctcatgcg cgagggcgtc cccggcatgg gcctcaacgt cgatgggcct tacttcaact
     4321 accaccacac gccggccgac accgtcgata agatcgaccc gcaggacatg gccctctgcg
     4381 tcgccgcgat ggccgtgatg gcctacgtcg tcgccgacat gcccgcctct ctcaacaagt
     4441 aaggcgcaag ccaccggcgt gacgcaacga gcgccggagg cgcggccaga tgtagcccac
     4501 ggcgtgagcc gtgggtagac cgttgacaag aaatttgaaa gccccggagg ggcgaaagaa
     4561 ccgcgatgag actcgtgctc aacgtcgcga ttctttcgcc cctccggggc ttccgtgtat
     4621 tttcaaccgc tattccccgg tctcacgacc ggggctacat tcttccgccg ctttcagcgg
     4681 ctcgttgccg cccgcggggg ttgctgcgtt tgtcgttacc gctgcgtcac gggtcggccc
     4741 tcggcgtcga ggatgacgat ctcgccgagg ttcagctcgc gcacgccggc ctcgatcttc
     4801 gagaggtcgc cgacgaggat gagcgtcgat ttgccgggca cggcgtactt cacggcggca
     4861 gcgttgacgg ccgcgagcgt cgcccgctgc gtctcgtccg gcagacgctg caactccgtg
     4921 agcggaagac cgttggccca gagctgggcc acctgcccga cgagctgtcc cagagactcg
     4981 aactgctgcg cgtagccgcg cacgcggttg cccttggcgt cggccagctc ctcggccgtg
     5041 attggcttgc cgcccgcaat cgccttcagc tcgctgacga actcgaccag agactccttc
     5101 gtcttgtccg tctgcacgcc gccggcggcc acccagatgc tcccgcggtc gtactgcacg
     5161 gggttcgaga aggtgccgta ggagtagcct ttctgctcgc gcaaattcag gttgaggcgc
     5221 gactggaagc cgccgcccca gacggcgtcg gcgaggttga aggcgtagaa ctcgtcggac
     5281 ttgcgctgcg gggcgccgag gacttgcagg acgaccgtct gcgccgcgtc cggcttgttg
     5341 atgagataga ccttgccgcc gctcgcgaac tgcggcgcgg cgatgttctg cgcgggcggg
     5401 gccgcgcccg tccacgagcc gaagtgctgg cgggcgagcg ccgtcgcctc ggccaccgtg
     5461 atgtcgcccg cgaagaagag cgcggagttc gacggcttca gataggtctg gtggaagcgc
     5521 gcgaggtctt ccggggtgat cctggcgacg gtcgaagtga agccgagacc gggacggccg
     5581 taaggatgat ccgcgccgaa gacgaccatg ttgcgcacgc ggttggcgat gccgttgggg
     5641 tcgttcatct gctgcgtgat gttatcaagc gtgatctttt tctcgcgctc gatctccgag
     5701 gccgggtacg tggggttcaa gaccacatcg gcgaagaggg tcatcgccgg gttgaggttg
     5761 cgtttgagca cctcgaagcc ggtggtggac gactcgcgcg aggggccggc gaagatggcg
     5821 gtgccgaggt cgccgagcgc gtcctgaatc tggatggcgt tctttgtctt cgtgcccatg
     5881 tccatcgtcg cgtaggccat ctgcacgagg cccgctttgt cggcggggtc ggccgcggtg
     5941 cccacgcgcg tcgagagcgc cacggccacc tttggcaatt cttttcgctc gacgacgaaa
     6001 atctccagcc cgttttccag tttcgccgtc ttcacctcgg gcgcgctgta cgccttgtcg
     6061 gcgcccatcg cgggctgctt cgagcggtcg aggtcggcct tctggggaag ctgcgaggtc
     6121 tcggggtgga agcggacgag gaggcggttg cgcgtgttga gccagcgggc ggctgtgtcg
     6181 cgcacgccct cggccgtggc gttgcggtag cggcccaaat cgaagtcgaa cttgttgggg
     6241 tcgccgaggt aggtgttgta gagattgagc aggtcggact tgccgccaaa acccccgatg
     6301 cgctcaagac ccgagatgta ctgggattcg agcttgacct tcgcgcggtt gagttcggcc
     6361 ggggtcggcc cttctttggc gaggcgggcg atctcgtccg tgatggtctg ctcgatctgc
     6421 ggaagcgacg cgccggggcg ggccgtggcg atgatggtgt agatgctggc gatctcgcgc
     6481 gactgctgga aggccgaaac gtccgaggcc acctgcttgt cgtagacgag cgtcttgttg
     6541 agacgtgaag agaggccgtc ggtcaggatc gtggagatga tgtcgagttc gccgtcgccc
     6601 ggctcgaagc gcggcgccgt gacccagttc atatagacgc gctcctgcgg cacgcggtcg
     6661 ttgacctcga tgatcttctc gcccacgagc gtggtgacgt tgcgggtcgg gcggtcgagc
     6721 gaggggccgg gggggagcgg gccgaagtat ttctcgacga gccgcttggc ctcggccggg
     6781 tcgaagtcgc cggcgacgac gagcgagagg ttattcggcg tgtaataggt cttgaagaaa
     6841 tctttgacgt cgtcggcggt cgcggccgtc aggtcttcgt ggctgccgat gaccgtccac
     6901 gagtaggggt gtcgggccgg gaagatgttt tcggtgacga gcgtgtccca gcggccgtaa
     6961 gggacattct ccaagccctg ccggcgttcg ttcttcacca cgtcgcgctg gttgtcgagc
     7021 ttcgtcttgt cgagcacgtc ggtgagcgtg gcgatgcggt cggcttcgag ccagaggagg
     7081 tattcgaggt tcgacgaggg cacggtggcg aagtagttgg tgcgatcctc gttggtcgtg
     7141 ccgttgacgc cgccggtgaa gaggttggcg ccggccttct cgacgtagga gaagtattcg
     7201 ccgggcgcgt tcttcgaccc ctgaaacatc atgtgctcga agaggtgggc gaagcccgag
     7261 cggttcacct tctcgttctt cgagccgacg tggaaccact ggttgacgtg gactacgggg
     7321 agcttccgat caacgtgcag gatgacctga aggccgttgg gcaggctgta cttctcaaac
     7381 tggattttgg ggacggcgct gtcctgcgcc gcgacggcga gcatctgcga ggcgacgacg
     7441 agcgcggcga caagcaggcg cagggtcttc ttctgaaaca tcttcacgag gtgactcctt
     7501 tcaaaattct ggtgcaaatc tggaagacag agaaacgcct aaacgtaaag gtgaagcgga
     7561 tagtttctcg actgagtggg ctggcttgta aaaacttccg gggacggacg ccaagactac
     7621 acgctgccgc ccgccgctgt caaaagtcta agcagccggc gtcggcgagc acgcgcggca
     7681 atctaaaaag cggcgtctta gtaataaccg ggcgtggtct gtataatccc caccgccccc
     7741 cgagaacttc ccctccgtcg ccctttcatt cgacccggcc ggataagtgt acctatgccc
     7801 aatgaacttc agcgggagga accgcccgac catgaaacag cgcatcgtcg gggggccgac
     7861 ttgcatggcc ccgagctggc gccctactgg ctcctggcca tcatcgagtc ggcggacgac
     7921 gccatcatca gcaagaccct tgagagcatc atcacgagct ggaacaaggg ggccgagcgc
     7981 atcttcggct acacggccca ggaggcggtc ggaaagcccg tcacgatgct catcccggcc
     8041 gaccatctgg acgaggagcc ggccatcctc gcgcgcatca gggccggcga ccgcatcgag
     8101 cactacgaga ccgtccgcgt ccgcaaggac ggcacgccca tcgacatctc gctgaccgtg
     8161 tcgcccatcc gaaacgccgc cggccaaatc atcggcgcct ccaagatcgc gcgcgacatc
     8221 agcacgcgaa ggcaatcgga gctggcgcag gcgcagctcg ccaacatcat cgagtcggcg
     8281 gacgacgcca ttatcagcaa gtcgctcgac agtatcatca cgggttggaa caagggggcc
     8341 gagcgcatct tcggctacac cgccgaggag atcatcggca aatccgtcac gatcctgata
     8401 cccgcggatc accccgacga ggagccggcc atcatcgcgc ggcttcgccg gggcgagcgc
     8461 atcgagcact acgagaccgt ccgcatccgc aaggacggca cgcccatcga catctcgctg
     8521 accgtgtcgc ccatccgtaa tgccgccggc cagatcatcg gcgcctccaa gatcgcgcgc
     8581 gacatcactg gcctgaagcg cgccgagcgc gagcgcgagc ggcttcataa gagcgagcgg
     8641 gaggcgcgcg agcgggccga ggagttgagc cacctcaagg acgagttcct cgccaccgtc
     8701 tcgcacgagc tgcggacgcc tttgaccgcc atcctcgggt gggcgcacat gctccgcacg
     8761 gggcagtccg acggcgactc taacctcaag gccttcgagg ccatcgagcg gaacgcgcgc
     8821 tcgcaggcgc agctcatcga agacctgctc gatgtctcac gcatcatcac cggcaagctc
     8881 agaatagatg tccggccgtt agaccccggt tcattcatcg actcggccat cgaggccgtc
     8941 aggcccgcgg cggaggccaa gggcgtgcgc gtgcagaaga tcatggacac gggcgccgtc
     9001 tccgtcgccg gcgaccccgt tcggctgcaa caggtgttct ggaatctgct cacgaacgcc
     9061 atcaagttca ccccgcgcgg cgggcgtgtg caaatcaggc tggagcgcgt caactcgcac
     9121 atcgagatcg ccgtcgccga caccggcgcg gggatcacgc ccgaattcct gccgcacgtc
     9181 ttcgaccgct tccgtcaggc cgaccagaag accacgcgcc gctacggcgg cctcggtctc
     9241 ggcctctcga tagtgcgcca tctggtcgag cttcacggcg ggacagttcg ggccgagagc
     9301 gccggcgagg ggcagggatc gaccttcacg gtgctgctgc cggtcgcccc cgtctaccaa
     9361 actggtgtcg aaggcgggcg cgtgcacccg gccgcgcggg acaccctgcc ctcttacgaa
     9421 tgtgccgaca ggctcgacgg gctgaggctg ctggtggtgg acgacgagca ggacacgcgg
     9481 gagttgttga aggcgggcct cgggcgctac ggggccgagg tgacggccgc cggctccgcg
     9541 gccgaggcgc tggaggcggt gcgggccgcc gtccccgatc tgctgatcag cgacatcggg
     9601 atgccgggcg aggacggcta cgacctgatt cgtcgcgtgc gcgcgttgag cgccgaagcc
     9661 ggcgggagag tgccggccat cgcgctgacg gcctacgcga gggtcgagga tcggatgcag
     9721 gcgctgcggg ccggttacca gatgcacgtc ccgaagccgg tcgagatggc ggaactggtg
     9781 gccgtcgccg ccagcctcgt ccgacgcggc aaatagtacg gcgtgaatga aagcggccgc
     9841 gccgctcggc ggcgtcttga ttattcaaat caagacgccg ccccttcggt ttacggcgcg
     9901 gctgtgaaac ccgtgagcgg gctgccatag aacgcgtcgg tcttccaggc catcttgttc
     9961 cacgctccgt cctgatctcc gatgatttgc acggggacga taacgcggtc gctgccgagc
    10021 ttgttggcgg aggcgatgcc gttgtcgaac ttcccgtccg tgccgacggt gaacttgatg
    10081 gagcgcgcca gcttgttatt ccacagtatc ttcaactcgt attcgcccgg actcgcgccg
    10141 agcgagtggg gcggcccgaa caggtcataa ttgtcccccg acttgtccca gccgacaatg
    10201 ttcgggaagc tgcacctgac gcgcgcccac ttcgccttct gcgggacggt gtcggcgacg
    10261 gattgcgtgg tgctgttctc cacgtccgac tcgcaaccgg ccctgccgac ctcctgaccc
    10321 tcgagagact tcttgcccac ctccttgccg ttgtggaaca ggtgaggctt gaagttatac
    10381 gcgtcgccgc gcacccagaa cgagacgtga aagtccgggc gctcccaccc cttcaaactg
    10441 ttagggacaa ggtagacgta accgatgggc aggttccagt cgtggttgac gaaatagacg
    10501 aacttgcccg cggccttggg gccgtgctcg ctggagaggg acttctccac tttcgtcttg
    10561 ccgttgaaga tagtggtgtc ggcgccggcc agctcgttgc gcagcttgat ggcgaagctc
    10621 accgggccgg tgtagagact gcccttgtct tcggggatgc cccggccccc gcactcggtc
    10681 ttccaccagc ggcccttctc cgtctcctcg gtcgggcagt cgaacttcac cgccggcgcg
    10741 ccggggatgg tgtattcgac gtagagctgg ccgccgctgg ggatcggccc gttgacgcga
    10801 aatgcgattc tgggaaccca actccacttg tcgtagtcac cgcgatagac gttgaaggtg
    10861 tagggggtca tctggatgga gtctttggcg atggtcggct gctcctggct cgcggcggag
    10921 cttccggccg aaagcagcag ggcgaacact gccgacgcca aaagtgtgaa gaccgaagtg
    10981 gacgcgatct tgtttctcaa gttgcacctc ctgaattttt ccacagttta aattttcaag
    11041 cgccgctcgg gatgaaggcg agcaatgcgg tcgggattgc cggggcgaaa atattatcga
    11101 ttcactttgt gtgtcaacga ttcaacgggc ttccggggtg gatttcggtc gggtgtcgtg
    11161 aaggggaggg gaacacggat gagacggatg agtacggatt gattcgtgtt aatccgtccg
    11221 caatccgtct catccgtgtt ccctccgccg tgcatcggcg gcgcacgccg tacacgcgaa
    11281 cgaagcgacg gggaggatgg tggcgggcgt gccgaacacg ccggcctcgt tgatggcggg
    11341 aagattccgg tagccgtcgt tcatgtcggt gccgtcgagc aggtagttga tcgagcggcc
    11401 gcgattgccg ttcgcgctga agaggccgaa ggagccgggc gagtcggtgg cgcccgaggg
    11461 gtcgccggtg gcgccgggga cgagcacgag aaacttggtg aagtcgcgcc cgttgatcgg
    11521 caggtcttcc acctgccgcg tcgggatcgt cccgccgagt gtgttgctgg tggactcgac
    11581 ctgcgtggtc gtggcgttga ccaccacgtt ctccgcgctg ccggagacga gcagcgacac
    11641 gtccgcgcgc cgctcgcccg cgacctcgac tgtggccccc acgaccctcc cctccgcaaa
    11701 gcccgtcgcg cgcacgacga catcgtaagt gcctatcggc agctcggcga tggtgtagtt
    11761 gccgtcggcg tcggtggtag ccgtgcgctc aagccccgtg ccggtgttcc gcgcggtcac
    11821 ggccgcgccc tgcacgaccg cgccctgcgg gtcggtgacg gtgccgagga tcgtgccgcg
    11881 gaaggtctgt gcggcgaccg gcgccgccac gagacacaga agcgcgacgc cggccgcgat
    11941 gagccggaac gaaaacgcgc ggcggcgggc cgtccgttga atgaagacaa ttctggtctt
    12001 tgacacgcga gtagtctcct tcgggattcg tctgtgtgga gcggcgaggg gctgcacgtg
    12061 aagcgtcacg acttataatc ccgaagcgcc gcgggcgtca acaaaatgct tggagcgcgt
    12121 cggccttgag tgccgacacg cacagtaccg cgagcggaag cctcgcgggt gcggccatga
    12181 tgagagaccc gccccgcgcg cgttagaccc gctcgctacc gcacgcggta ctgtgtcgcg
    12241 ctcacggcaa ggtgcgcggg tgttataatc gcgcgccgtt ttggttagga gggtttgagt
    12301 cgtaagtgct gattgatgac aacaacagga agctcatcgc gcaactgcgg cggacgatga
    12361 acgagctggc cgcgccgctc ggccagagca tccgcgtcgt ctggcagggc ggcggcgcgc
    12421 ccgacgcgcg cgccgtctta gacaaccagt tgctgctcac ggcccagcac ttcatcgatc
    12481 tgcgcgaggc gtcggacgag gagaagcagc actactacgg gcaggtctgc gccgtcttcg
    12541 ggctgcgggc cgaggacaat acctttccgc tctcgcgctc gcgcgacctg ccgccgcacc
    12601 tgctgcgccg gctcgaccgc accccggcgt gcgtctccta cctcgacacc ttcgacgcgc
    12661 gccacggcac ggcccacgcc gaccgcgcgc gccgctgtct cttccagttc gccaacctcg
    12721 tcatcaagtc cgacggacgc atcacgccgg ctgaggagct ggcgctcgtc gagttcaagc
    12781 acgcgctcta ccccgagggc gcgcccgcca gcgccgtggg cggcgccggc gccacggccg
    12841 aagccgaagc cgcgcccgaa gaggagctgc ccccgccgcg cccgctcgaa gagcttttgg
    12901 ccgaactcga cgcgctcgtc gggctggagc gcgtgaaggc tgacgtgcgc cagatagcca
    12961 acttcatccg cgtgcagaag atgcgcgagg agaaaaaact caagacgctt cccgtctcgc
    13021 gtcacctcgt cttctacggc aaccccggca cgggcaagac cacggtggcg cgcctgctct
    13081 cggaaatcta tcgctcgctc ggcgtcgtgc ggcgcggcca cctcgtcgag accgaccgcg
    13141 ccggtttggt cgccggctac gtcgggcaga cggccatcaa gacgaaggag gcggtcgcgc
    13201 gctcgcgcgg cgggatgctc ttcatcgacg aggcgtacac gctctcctcg ggcggcgcgg
    13261 gcgacttcgg ccgcgaagcc gtcgagacgc ttttgaagct gatggaggat caccgcgacg
    13321 acctggtcgt cgtcgtcgcc ggctacacgg gcaagatgca ggagttcctc gattccaacc
    13381 cgggcctgcg ctcccgcttc aaccggcatc tgcgcttcga cgactacgac accgcgcagc
    13441 tcgtcaaaat tttccagacc ttctgcaagc gggccgactt ccgcatcacg cccgaggccg
    13501 aagcccagct cgccgcgcgc ttcgacgtgc tctgtctcat gcgcgacgag accttcggca
    13561 acgcccgcac cgcccgcaac atcttcgagg tcgccatcag caagcaggcc aaccgtgtcg
    13621 tcgcgctcac ggacatcacg gccgacgcgc tctcctgcat cgagccggcc gacatcccga
    13681 cggaggagga gctgcgcgcc tgcggcatca tcacgcccga acgttagagg cgcggggggt
    13741 gggccgcttc ggggcgggag gctggtacgg tgaggatgga atccgagggc gcgcttcgga
    13801 acgtataatt tggcggccgg aggggcgctt ggaagcgcgc ctccgttaaa aattctggac
    13861 ggcgcgcgcc aatctgctta caattatgtc tcccttgcac gtctcccttg cagtagtccg
    13921 cagtcccgcc gaggtaatta attatgtcta gagtcacacg cgtcgcgctc tggctcattc
    13981 tgggcctctc cctgtcggcc gcgtgggcgc cggccgcccg cgcgcgctcg tccgccgtca
    14041 acactacgcc ccatgtcgcc cttcagggca gaaaccgcat ctccggtcac gtcttcgccg
    14101 aggcgcgccg gccggtgccc gacatttacg tcgagctgat ggacgaggtg tacacgacca
    14161 tcgcccgcgc gcgcaccaac ggctcggggt tctactcgtt tgaaggtctc tccaccggca
    14221 acttcaagct caaggttctg ccctacggca ccgacttcga ggagcagacg agagacatca
    14281 ccatctacaa catctcggtc gtttccggcg ccggggccag cagcgagcag gtcgatttct
    14341 atctcaagac gcgtgggagc ctcgtcacca gccccttcgc ggcgccgggc acggtcttcg
    14401 cgcaggacgt gccggaagag gccaggaagc actatgagcg gggcgtcgcg ctgctgggcg
    14461 agaagaagga caaggaagct ttcgatagtc tgaagcggtc gctggaaatc ttcccgcgct
    14521 actacatggc gcttgaccgt ctgggcaccg agtacgttct gcgcggctac aagacggcgg
    14581 cctacatcct cctgacggag gcggtcaagg tcaacccgcg cggcttctcc agcctgttcg
    14641 gtctgggaat gtcggagttc cagttgggga tgatgaacga ggcggcggag agcttccggc
    14701 gggcgacgct ctcctacgac ggctcgatca acgcgcacat gtacctgggt ctggcgctga
    14761 agcggacggg gaagttggcg caggcggagg cgtcgctcaa gcgcgcgcgc gacgtgggca
    14821 agggccagac gccggaggtg cactggcacc tcgcccggct ctacggcgaa cagaagcgct
    14881 acgccgaggc cgccgactcg ctcgaactct atctgaagac ccagccgcag aaccccgacc
    14941 cgcagaaggt ggcgaagatt cagcaggtca tccgacaact gcgcgagaag gccactaaag
    15001 gttagaccga aaagcgcgag cggcgcgccg cccggatcgc ttggagttga gtgtagggtg
    15061 gttcggcttc accgcaagag gcgcaaagga cgcagagtcc aagcagaggg ttgaatctct
    15121 ccgcgtggac tctgcgtcct ttgcgcctct tgcggtgagt taatcctgac tcacactcaa
    15181 gtgaaaagtg ccctagttgg cgcgcgaggt gctctcagac gcgccggtcg cgcccttgag
    15241 gtgcttgtcg aggaattcga gaatcgcctt gttggcgcga atctcgttga ccttcttcgt
    15301 aaagccgtgc ccctcgttgt cgaagacgac gtactcgacg gggacgttgt tcttcttcac
    15361 ggcttcgacg atctcgtccg actccggcag gatgacgcgc gggtcgttct tgccctgcaa
    15421 cacgatgagc gggcggcgta tcttatccgc gtggaaaagc ggcgagatgg cacgcaggtt
    15481 ttcgaggtcg gttgccgggt cgcccatctc tttgtagagg gctttacgga acgattccca
    15541 gtagggcggg atcgactgaa gcgtgcgcac ccagttcgac acgccgaaga tgtccacgcc
    15601 gacagcgaac tcagcgggct tgaaggcgag cgcggccagg accatgtagc cgccgtaaga
    15661 gccgccgatg atgccgatct tcgattcgtc aacatagccg agcgaggcga gatatttttt
    15721 cgcttcgatg caatcccata gaggctcgcg cccgtgcttg ccgtcgtccg ccgcgaagaa
    15781 ggttttgccg tagccgctcg aaccgcgatt gttgacgccg aggatgacgt agcc
//
LOCUS       DASVES010000370         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_643058, whole genome shotgun sequence.
ACCESSION   DASVES010000370 DASVES010000000
VERSION     DASVES010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643058"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..>2506)
                     /locus_tag="VF240_15200"
     CDS             complement(202..>2506)
                     /locus_tag="VF240_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176610.1"
                     /translation="DFLTAALVAAGLDLRAALLCTAVPLALALTGIIYTFALRVLREG
                     DDELHAAPVAEALVMQNDSAVNAEAEPATNERELETTPDAAGARRLTGKEACVSAALA
                     TLLFLLNGGLGFLYFYGDWRGSGRSLAQFWQTLAINYANMPERQIYWTNLISDTLLPQ
                     RTSLFGMPAALMIFTAFAIVWRGWSARVVGNRDDDGVAGVRDAWAGWRVLFAAGVLAG
                     LLPLFHAHSFGAVGFVSGLLFLLRPRRAWLAFWLPAVALALPQFAGLALHLAGNGFMR
                     LQTGWRYDGGSAWGFLIFCLTNVGVPALLVVPAWLAAPRVWRRFHLAFAGLLVFSLVV
                     VVSPNDYDNIKLMYYWYAHACALVAAWLVRLATRARAGFRVLAATLALASVASGLLAL
                     RHESANVKLLYSADEVEAARFVREHTPPRTLFLTAPAVHQPILSLAGRAVVRGDTAWL
                     WSHGYDFPARERDVRAIYTGSDDAATLLSHYRIDFVYLGRRERESLRAEEAFFKVNLP
                     VFYRRGDITIYDARPLTRPPAGDETARPAGTKRPVSEDAERGASVAHLPFASPPPREF
                     AARLERDPAQFLVEFPRAAFAVYRYHMVARGRWPEYREFMEDMRHVGSGVRVGAAGWE
                     RQLEANKLELTDAWPAREEFRRAFDGLGAGEFIDALYKNAGVELSRTEHNRLAAALEA
                     GAETRASLLRHVAEERELFSRDYDTAYVLTHYFGYLRRGPADPPDKDLDGFNFWLRIL
                     QRTRDYRSVTRVFIESGEYKDQGKGQK"
BASE COUNT          425 a          805 c          918 g          358 t
ORIGIN      
        1 ccctgaactc gctccacgcc gaccgcagcc gcgccctccg catgggtgag cgcggccgcg
       61 agaaactgct cgcgatgaat ctctcctggg acaacgtggt cgaacgcctc atctccgcgg
      121 cggagtaagg caaaagtaaa aaggcaaaag gcaaaagtga aggcgccgga cgcgcgttat
      181 gtcctcttca cttttgcctt tttacttttg ccctttgcct tgatctttgt actctcccga
      241 ctcgatgaag acgcgtgtga cgctgcggta gtcgcgtgta cgttggagga tgcggagcca
      301 gaagttgaag ccgtcgaggt ctttgtcggg ggggtcggcg gggccgcggc ggaggtagcc
      361 gaagtagtgc gtgagcacgt aggccgtgtc gtagtcgcgc gagaaaagct cgcgctcctc
      421 ggcgacgtgg cggagcaggg aggcgcgcgt ctccgcgccc gcttcgagcg ccgccgcgag
      481 ccggttgtgc tccgtgcgcg aaagctcgac gccggcgttt ttgtaaagcg cgtcaatgaa
      541 ctcgcccgcg ccgaggccgt cgaatgcgcg tcgaaattcc tcgcgcgcgg gccacgcgtc
      601 cgtcagctct agcttgttgg cttcaagctg gcgctcccac cccgcggcgc cgacgcggac
      661 gccgctcccc acgtggcgca tgtcctccat gaactcgcgg tactccggcc agcgcccgcg
      721 ggcgaccatg tggtagcggt agacggcgaa ggccgcgcgc gggaactcga cgaggaactg
      781 cgccgggtct cgttcgagac gcgcggcgaa ctcgcgcggc ggcggcgatg cgaaggggag
      841 gtgcgcgacc gacgcgccgc gctcagcgtc ttcgctcacg ggcctcttcg tgccggcggg
      901 ccgcgccgtc tcgtcgcccg ccgggggccg cgtgaggggg cgcgcgtcgt agatggtgat
      961 gtcgccgcgt cggtagaaga ccgggaggtt gaccttgaag aaggcttctt cggcgcggag
     1021 tgattcgcgc tcgcggcgtc cgagatagac gaagtcgatg cggtagtggc tcagaagcgt
     1081 ggccgcgtcg tcgctgccgg tgtagatggc gcgcacgtcc cgttcgcgcg cggggaagtc
     1141 gtagccgtga ctccagagcc acgccgtgtc gccgcgcacg acggcgcgtc ccgccagact
     1201 caggattggc tgatggacgg cgggcgcggt caggaagagc gtgcgtggcg gcgtgtgctc
     1261 gcgcacgaag cgcgcggcct cgacctcgtc ggcgctgtag agcagtttga cgttcgcgct
     1321 ctcgtgccgg agcgcgagca ggcccgaggc gacagaggcg agggcgagcg tcgcggcgag
     1381 cacgcggaag ccggcgcgcg ctcgcgtggc gagacgcacg agccaggcgg cgacgagcgc
     1441 gcaggcgtgc gcgtaccagt agtacatcag cttgatgttg tcgtagtcgt tgggcgagac
     1501 gacgacgacg agactgaaga cgagcagccc cgcgaaggcg aggtggaagc gccgccagac
     1561 gcgcggcgcg gcgagccacg ccggcacgac gagcagcgcg ggcactccga cgttagtcag
     1621 acagaagatg agaaagcccc acgcgcttcc gccgtcgtag cgccagcccg tctggagacg
     1681 catgaagccg ttgccggcga gatggagcgc gaggccggcg aactgcggga gcgcaagcgc
     1741 gacggccggc agccagaagg cgagccacgc gcggcgcggc cggagcagga acagcaatcc
     1801 gctgacaaaa ccgaccgcgc cgaacgagtg cgcgtgaaag agcggcaaca gaccggcgag
     1861 cacgcccgcc gcaaacagca cgcgccatcc cgcccaggcg tcacgcacgc cggcaacgcc
     1921 gtcatcatcc ctattcccca cgacgcgagc cgaccagccg cgccagacga tggcgaacgc
     1981 cgtgaagatc atcagcgcgg cgggcatgcc gaagaggctc gtgcgctgcg gcaggagcgt
     2041 gtcactgatg aggttcgtcc agtagatttg ccgctcgggc atgttggcgt agttgatggc
     2101 gagcgtctgc cagaactgcg cgaggctccg gccgcttccg cgccagtcgc cgtagaagta
     2161 gaggaagccc aggcccccgt tgagcagaaa gagaagggtc gcgagcgcgg ccgacacgca
     2221 cgcctccttc cccgtcaaac gtcgcgcgcc ggcggcgtcg ggcgtcgtct ctaactcgcg
     2281 ctcattcgtc gccggttccg cctccgcgtt cactgccgag tcgttctgca tcacgagagc
     2341 ttcggcgacc ggcgcggcgt gcaattcatc gtcgccttcg cgcaggacgc gcagggcgaa
     2401 cgtgtagatg atgccggtga gcgcgagcgc gagcgggacg gcggtgcaga ggagcgcggc
     2461 gcgcaggtcg agacccgcgg cgacgagcgc ggccgtgagg aagtcg
//
LOCUS       DASVES010000371         7127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_49730, whole genome shotgun sequence.
ACCESSION   DASVES010000371 DASVES010000000
VERSION     DASVES010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49730"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF240_15205"
     CDS             <1..205
                     /locus_tag="VF240_15205"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX7176611.1"
                     /translation="EFDRLFLTGMIQHHDGALMMVKDLFDTAGAGQDAELFNFATDVD
                     SGQRAEIRIMQTMLGEKPVEEKR"
     gene            202..2220
                     /locus_tag="VF240_15210"
     CDS             202..2220
                     /locus_tag="VF240_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176612.1"
                     /translation="MNRVHTIAWRLLTASIVLCSCFAAGTVAQTASPSPQPSPTPTPE
                     AKPASQEGANPFTPEAAPPLPAGMTGSDTGDPRSRLSPGMYEAGEAAMGIKHLMLLKK
                     PDAFQLGSDDPDHPKVQKALSLLGVADASKIPKSQQLATAGLAFANSDLAFQGNHLFL
                     GNFYGVNIYDISDPLKSRLLTSMVCPGGQGDVSVYKNLLFMSVEQPNGRLDCSPQGFP
                     PGPPPAAGQEKNPPPPAAQKDRFRGVRIFDIADIRNPKQVASVQTCRGSHTHTLVVDP
                     KDKNNVYIYVSGTSFVRQSEELAGCSGETPDKDPNTALFRIDVIKVPLQAPQSAQIVS
                     SPRLFMDPRTGALNGLSSGGTHGKDGLEKPSDTDQCHDITVYSAIGLAAGACSGNGIL
                     LDIKDPVRPKRIDAVNDPNYAYWHSASFSNDGKKVVFTDEWGGGLGARCRANDPNKWG
                     ANAIFRLANNKLSFASYYKLPAAQGDSENCVAHNGSLVPVPGRDIEVQAWYQGGISVM
                     DFTDAEHPVEIAYFDRGPIDPKMLVLGGAWSAYWYNGHIYSSEIARGLDIFELTPTQH
                     LTQNEIDAAKAVRVAAFNVQNQEKIEWPRRLVVAKAYVDQLERSQALPADRIADLRQA
                     IQSAESRKELGKLKSLVPSLEQSAGLTKSAADSSRLRALAEILRRPAL"
     gene            2341..3000
                     /locus_tag="VF240_15215"
     CDS             2341..3000
                     /locus_tag="VF240_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7176613.1"
                     /translation="MKRLLRGILGFALAYVLVGAVLDRVIFPETEPGPDYYPRQGQTF
                     VSETEGFRQTILKREGGLAWVELVLEPRAPGPPEHIHTSFAENFIVAEGALSLLVNGE
                     KKLLRAGESLLVTPGTPHKPFNETDSRVVVRGPLTAEYGMPEQFTVFLTQAYGFFDES
                     ESNGRPPKALMQMSRFSPKYDVWLAGPPVALQKAVFFIIGPTARLFGYRTRYEKYVPN
                     S"
     gene            3097..4851
                     /locus_tag="VF240_15220"
     CDS             3097..4851
                     /locus_tag="VF240_15220"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176614.1"
                     /translation="MRRREARLAEAARRMQRFRKVGFLRLAALAAGLVLIWLVIGGLA
                     FWWLLPPAVVFLALAEVQARISRERRRCERAADLYDEGLARLDDRWAGKGATGERFLD
                     GPHPYAADLDLFGPGSLFELLSRARTRAGEETLAHWLLHPAPPSVVRARQAAVSELRS
                     RLGLREDLALLGEGVRSGEDAHSLAAWATAPPWPVSTGLRATAIALALLAVVTLILWI
                     AGFGGTPFLVALIMGRAFAFRYRVPVSRVVNAVDAAGRDLALLSGVLGRLEQERFTSP
                     RLAELRAALDVEGLAASRQIARLNGLIDLLDAQRNAMFAPLAFLLLWPVQLAIAFEGW
                     RRSSGAAVVRWLAAVGELEALSSLAGHSYERPQDPFPELAEGGACFEGEGLGHPLIPE
                     SRNVRTDLRLSDERLRVLIVSGSNMSGKSTLLRTVGTNTVLALAGATVRARRLRLSPL
                     QVGASIRIQDSLQAGASRFYAEITRLRQIVGLTGEPPPVLFLLDEILHGTNSHDRRIG
                     AEGVVRGLIERGAIGLVTTHDLTLARIADELAPQAANVHFEDHLEGGKMSFDYQLRPG
                     IVQRSNALELMRSVGLEV"
     gene            5069..5476
                     /locus_tag="VF240_15225"
     CDS             5069..5476
                     /locus_tag="VF240_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176615.1"
                     /translation="MLLIKTALTLASVLAPLLQAGCGSAPPHRPSESKPRVVAPAQSA
                     SSDHQRIVEKISAIVAKQLDLEVREVDVNVPLSKQKKAADELDVVEIVMNVEDAFGIE
                     IKDEEIGESLEVVSRDLSVKKLADIVSKKRSPK"
     gene            5881..>7127
                     /locus_tag="VF240_15230"
     CDS             5881..>7127
                     /locus_tag="VF240_15230"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX7176616.1"
                     /translation="MAIGCLGAPMVWAQGDYSAVQRNLPTREAQQRLVAGLTPVRPKM
                     RLRRALNAPKPAPPSRQRQEYDYSSTPEARRGVAELEKVKRNIVRSDWAVEARPGKER
                     VIFASAEGGEQELEYDTAELTALATRAAPRGINHASVGGVRGAELEQPSPFMLAGWSG
                     GADNRIVKPINATYPINHRVLMRIGELNENCSGALVGRRLVLTAAHCIVQPDLSYNTH
                     AFRARRSGTQMPYGTATSVGYWYAAKWVSNNCHVNRTWECSPHDWAIILLADNAWNNS
                     PNGTPGWMGYWVYGQNYIQQNAVSHNDGYPASWWSNKPANHQVNQPYGQTAACTATGF
                     EWPFDGVASYYRLGCDMSGGHSGSPNWTDYPGSNGPYVIGIAMWSHCFECTDATGTWK
                     SHPSGFRGMTHYLANLITNKRIA"
BASE COUNT         1391 a         2169 c         2219 g         1348 t
ORIGIN      
        1 ggagttcgac cgtctgtttt taaccggaat gattcagcac cacgacggtg cgttgatgat
       61 ggtcaaggat ttgttcgata cggcgggcgc ggggcaggat gcggaactgt tcaatttcgc
      121 gacggacgtt gacagcggcc aacgtgctga gattaggatt atgcaaacca tgctgggtga
      181 aaaacctgta gaggagaaac gatgaaccga gtccacacta tcgcttggcg tttactaaca
      241 gcttccattg ttttgtgctc gtgtttcgca gccgggacgg tggcgcaaac ggcctcgccg
      301 tcgccgcagc cttcgcccac gccgacgccg gaagccaaac ccgccagcca ggagggcgcg
      361 aatccgttta ctcctgaagc ggcgccgccg ctgccggcgg ggatgacggg ttcagataca
      421 ggcgacccgc gcagccgtct gtcgccggga atgtacgagg cgggtgaagc agcgatgggc
      481 atcaagcacc tcatgctgct caaaaagccc gatgcgtttc aactcggctc cgacgatcct
      541 gatcatccga aggtgcagaa ggcgctgagc ctcctcggcg tcgccgacgc ctcgaagata
      601 ccaaagtctc aacaactggc tactgccggg cttgctttcg ccaactcgga ccttgcgttc
      661 cagggcaacc acctgtttct gggaaacttt tatggcgtca acatctacga catctccgat
      721 ccgttgaaga gcagattgtt gacttcgatg gtgtgtccgg gcgggcaggg cgacgtgtca
      781 gtttacaaga acctgctctt tatgtcggtg gagcagccga acggccgact cgattgcagc
      841 ccacagggtt ttccgcccgg gcctccgccc gccgcgggac aagagaagaa tccgcctccg
      901 ccggctgcgc aaaaagatcg tttccgcggg gtcaggattt tcgatatcgc cgatatcagg
      961 aatccgaagc aggtggcctc ggtgcaaacc tgccgcggct cgcacacgca tacactggtg
     1021 gtcgatccga aggataagaa caacgtctac atctacgtct cgggaacctc ttttgtccgt
     1081 caaagcgaag agctggccgg ttgctccggt gagaccccgg acaaggaccc caatactgcg
     1141 ctctttcgca ttgacgtgat taaggtgccg cttcaggcgc cgcaaagtgc tcagatagtg
     1201 tcgagtcctc ggctgtttat ggatcccagg acgggcgctc tgaacggcct cagtagcggc
     1261 ggcacacacg gcaaggacgg attggaaaag ccgtccgaca cagaccagtg ccacgatatt
     1321 accgtgtact cagccatcgg actcgcggcc ggcgcctgct cgggaaacgg aattctgctg
     1381 gacatcaaag acccagtccg tccaaagcgc atagacgcgg taaacgatcc gaactatgcg
     1441 tattggcact cggcctcgtt ttcgaatgac gggaaaaaag tcgtgtttac cgatgagtgg
     1501 ggcggcggac ttggtgcgcg ctgccgcgcg aacgatccga acaaatgggg cgccaatgcg
     1561 attttccgcc tggcgaataa caagctgagc ttcgcgagct actacaagtt gccggccgcg
     1621 cagggcgaca gcgagaactg tgtcgcgcac aacggctcgc tcgttccggt tcccggccgg
     1681 gacatcgagg tacaagcctg gtatcagggc ggcatctccg tcatggactt caccgacgcg
     1741 gagcatccgg ttgagattgc ctatttcgac cgggggccga ttgatcccaa gatgctcgtg
     1801 ctcggcggcg cctggtccgc ttactggtac aacggccaca tctactcgtc agagatcgcg
     1861 cgcggcctcg acatctttga gctgacgccg acgcagcacc tcacgcagaa cgagatcgac
     1921 gccgcgaaag cggttcgggt ggctgcattc aatgtgcaga atcaggagaa aatcgagtgg
     1981 ccccgacggt tggtcgtagc gaaggcgtat gtcgatcagc tggagcgttc gcaagctctg
     2041 cctgctgacc gaatcgcaga cctgcggcag gcgattcaaa gtgcagagag ccgaaaagag
     2101 cttggaaaac tcaaaagcct cgtgccgtcg ttggagcaga gcgccggcct gacgaagagt
     2161 gcggcagact catctcgatt gcgagccttg gctgaaatcc tgaggcgacc cgcgctttga
     2221 tcgtttcgag cgcaagctcg ggcgctgttt gagaatacgc gccgagagcg tcaactcgaa
     2281 ccgttaggcg ctggcgactt acgaagctgt atggcaagta ggctggtgtt tctattgaag
     2341 atgaaaaggc tgcttcgggg gattctcggc ttcgctctgg catacgtcct cgtcggcgcc
     2401 gtgctcgacc gggtcatctt cccggaaact gaacccggcc cggactacta cccgcggcaa
     2461 ggtcagacgt ttgtcagcga gaccgaaggt tttcgccaga cgattcttaa acgcgagggc
     2521 ggtctcgcgt gggttgaact ggtgctggag cctcgcgctc cggggccgcc cgagcacatc
     2581 cacacctcct tcgcagaaaa tttcatcgtg gccgagggcg ccttaagcct cctcgtcaac
     2641 ggcgaaaaga aactcctgcg cgccggggag tcgctgctgg taactcccgg aaccccgcac
     2701 aagcctttca acgagaccga ttcgcgtgtg gtggtcagag gcccgctgac tgcggagtac
     2761 gggatgcccg agcagttcac tgtctttctc acacaggcgt atggattctt cgacgagtcg
     2821 gagtcgaacg gccgaccgcc gaaggctttg atgcagatgt cgaggttcag ccctaagtac
     2881 gacgtgtggc tcgccggccc gccagtcgca ctacagaagg ccgtgttctt catcatcggc
     2941 ccgacggccc ggctcttcgg ttatcgcaca cgatatgaga agtatgtgcc gaacagttag
     3001 ctcagaatgg tttaagctgt aaggatgaat gagacagagg atggcctctt aaacgaatta
     3061 acggaacaca gggcggcgga accccggctt gaatatatgc gccgccgcga agcgcgcctc
     3121 gcggaagcgg cgcgccggat gcagcgtttt cgtaaggtcg ggtttctgcg cctcgccgcg
     3181 ctggccgccg ggttggtgct gatctggctc gtcataggcg ggctggcctt ttggtggctg
     3241 cttcctccgg cggtcgtctt cctcgccctg gcggaagtac aggcgcgcat ttcacgggag
     3301 cggcggcgct gtgagcgcgc ggccgacctt tacgatgagg ggctggccag actcgatgat
     3361 cgttgggccg gaaagggggc gacgggcgaa cgcttcctcg acggccctca cccctatgcc
     3421 gcggacctcg atctcttcgg ccccggatca ctcttcgaac tgctttccag agcgcgcacc
     3481 cgcgccggcg aagagacgct ggcccactgg ctgctccacc ccgcgccgcc cagtgtggtg
     3541 cgcgcccggc aggcggccgt gtctgagctg cgctcacggc tcggtttgcg cgaagacctc
     3601 gcgctgctcg gcgaaggcgt ccgctccggc gaagatgctc attcgctcgc cgcgtgggcc
     3661 actgccccgc cgtggcccgt ctcaacgggg ctgcgcgcga cggcgatagc gctggcgctg
     3721 ctcgcggtcg tcactctcat cctgtggatt gccggattcg ggggcactcc ctttctcgtc
     3781 gcgctcatca tgggaagggc gttcgccttc cgctaccggg ttccggtcag tcgtgtcgtc
     3841 aacgccgttg acgccgcggg gcgtgacctc gcgctgctgt cgggagtgct gggccgtctt
     3901 gagcaggagc gattcaccag tccccgcctc gccgagctgc gcgcggcgct cgacgttgaa
     3961 ggcttggctg cgtcacgaca gatcgcccgg ctcaacggct tgattgatct gctcgacgcc
     4021 cagcgcaacg cgatgtttgc gccgctggcc ttcctcttac tgtggccggt gcagctcgcc
     4081 atcgccttcg aaggctggcg ccgaagctca ggagcggccg tggttcgctg gctggccgcg
     4141 gtgggcgagc ttgaggctct cagttctctg gcgggccatt cttacgagcg tccgcaggac
     4201 cctttccccg aactggcaga gggcggcgcg tgctttgagg gcgaagggct tggtcatccc
     4261 ttgatccctg aatcacggaa cgtccgcacg gacctgcgcc tgtcagacga gcgactgcgc
     4321 gtgctcatcg tcagcggctc gaacatgtcg ggcaagagca cgctgctgcg cacggtggga
     4381 accaatacgg tgctggcgct ggcgggcgcg acggtccgtg cccgccgcct gcgcctgtcg
     4441 ccgcttcagg tgggggcctc gattcgcata caggattcgc tccaggccgg ggcctcgcgc
     4501 ttttacgcgg agataacgcg cctgcgccag atcgtggggt tgaccgggga gccgcccccg
     4561 gtgcttttcc tgcttgacga aattctgcac ggaactaact cgcacgaccg ccgcatcgga
     4621 gccgagggcg tggtacgcgg tctcatcgag cgcggcgcca tcgggctggt cacaactcac
     4681 gacctgacgc tggcgcgcat cgccgacgag cttgcgcctc aggcggccaa cgtccatttc
     4741 gaggatcacc tcgaaggggg gaagatgagc ttcgactatc agttgcgccc cggtatagtg
     4801 caacgaagca acgccctcga attgatgcga tcagtcgggc ttgaggtctg aatcgaagca
     4861 acagagcagc ggcgtaatca atgttgtttg accatatcgt cgtcgccgcc gagcaactca
     4921 ttcaacccga cgcctcgata gcattctttt catcgttgat ttattcttcc cccattgaat
     4981 acctgcgctc ggcgcgggtt aattccggcg ttagatgtgc tgcttgtctg aatcagtaga
     5041 ccattaggcc cgaatgttct gaggaaagat gctgctaatc aaaacggcat tgacgctggc
     5101 gagtgtgtta gcccccttat tgcaagccgg ctgcgggagc gcgccgcctc accgaccttc
     5161 ggaaagtaaa ccgagggtag ttgcacctgc tcaatccgcg tcctctgatc accaacgaat
     5221 cgtagagaaa atcagtgcca ttgttgccaa gcagctcgat cttgaagtac gcgaggttga
     5281 tgtgaacgtg cctttgtcga agcagaaaaa agcggcggat gagttggacg ttgtggagat
     5341 tgtcatgaac gttgaggatg ccttcggtat tgaaatcaag gacgaggaaa ttggagagtc
     5401 tctggaagtg gtcagcagag atttatctgt caaaaagctt gccgacattg tgtcgaaaaa
     5461 aaggtctccg aagtgaccgc gcatctcaca acggcatgca gcggacgcgc aaatcagtgg
     5521 ccgtcttaaa ttcaaggaaa gggagcgcga cggactcgtc gagcaggtga tcatcgtccg
     5581 catccgctac gacgacggcg ccgtctggca ggcccgttga gaagttgatg ttaatgaact
     5641 cgattccgta gaagacgccc ggctcgcccc gaactcattt acgacagcag atccgctggc
     5701 ggccgcaaga ataagtcgcc gggggaacgg cgaataggga tatgatgccg ctggcagcga
     5761 catgatggga gttcggcggg cgttacacgt ccgattccca ctctccacat acggccccgg
     5821 taaatgttct ccaaaggagg atgtgcgatg aagaccaggc ttgttacgtc agttgtagcc
     5881 atggcaatcg gttgcctcgg agcgcccatg gtttgggcgc agggcgacta ttcagccgtc
     5941 caacgaaacc tgcctacacg cgaagcgcaa cagagacttg tcgccgggct gactcccgtc
     6001 cggccgaaga tgcgcctgcg ccgcgccctc aacgccccga agcccgcccc tccctcgcgg
     6061 cagcgtcagg agtatgacta cagcagcacg ccggaggcgc gcaggggcgt ggcggaactc
     6121 gaaaaggtga agcgcaacat cgtcaggagc gactgggcgg tcgaggctcg acccggcaag
     6181 gagcgcgtca tcttcgccag tgcggagggc ggggagcagg agcttgagta cgacacggcg
     6241 gaactcaccg cgctcgcgac ccgcgcggcc ccgcgcggca tcaaccacgc gagtgtggga
     6301 ggcgtccggg gggctgagtt ggaacagccg tcgccgttca tgcttgccgg ctggtcgggc
     6361 ggcgccgata accgcatcgt caagccgatc aacgcgacgt atcccatcaa ccatcgcgtg
     6421 ctcatgcgta tcggagaact caacgagaat tgcagcgggg cgctcgtcgg ccggcgtctc
     6481 gtcctcaccg cggcgcactg catcgtccag ccggacctca gttacaacac ccacgccttc
     6541 cgcgctcgcc gctccggcac gcagatgccc tacggcaccg ccacgagcgt cggctactgg
     6601 tacgcggcca agtgggtctc caacaactgc cacgtcaacc gcacgtggga gtgttcgccg
     6661 cacgactggg cgatcattct tttggcggac aacgcgtgga acaactcccc gaacggcacg
     6721 cccggctgga tgggctactg ggtctacggc cagaattaca tccagcaaaa cgccgtcagc
     6781 cacaacgacg gctacccggc gagctggtgg tcgaacaaac ctgcgaacca tcaggtgaac
     6841 cagccctacg gccaaacggc cgcctgcacc gccacgggtt tcgagtggcc cttcgacggc
     6901 gtcgcttctt actatcgtct ggggtgcgac atgagcggcg gccacagcgg ctcgcccaac
     6961 tggaccgact accccggctc gaacggcccc tacgtgatcg gcatcgcgat gtggtcacac
     7021 tgcttcgaat gcaccgacgc gacaggaacc tggaagtcgc atccgagcgg cttccggggc
     7081 atgacccact acctcgccaa cctcatcacc aacaagcgca tcgccta
//
LOCUS       DASVES010000372        12771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1050742, whole genome shotgun sequence.
ACCESSION   DASVES010000372 DASVES010000000
VERSION     DASVES010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050742"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VF240_15235"
     CDS             <1..261
                     /locus_tag="VF240_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX7176617.1"
                     /translation="DDPFQVLDREGVGQLVRMGTERGRAAKPGLKVGICGEHGGEPRS
                     IAFCHETGLDYVSCSPFRVPVARLAAAQAALADRREAETTDK"
     gene            467..1819
                     /locus_tag="VF240_15240"
     CDS             467..1819
                     /locus_tag="VF240_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176618.1"
                     /translation="MRERLIAEYHELLKADETLTPEFFARLKGAMTVRRLVYGERQIG
                     VSLRPHLLTRAQYDDLARSSEVLAGAFEKITAALIANPSMMSEVGLNEREQSLALVNP
                     GFSNSSVTTRLDAFIGREGVKFVEYNAENPSSLTDQSGLNQILFEVRALQKISGRYRL
                     RQFDPAEALLDALLETFREWGGRSVVPNVAIVDWAGVPTEGEFILLRNYFVGRGVPTV
                     ICSPEELEYDGTRLRRGDFPVDLVYKRVIIHELLARCDDMHPLMRACRDGRVCLANPF
                     RCKIAHKKAAFELLTDDERGGWFTSEEREVVRRTVPWTRRVRERKTSHRGREVDLIEY
                     VRRERELFIIKPNDDYGGQGITLGPRAGEQEWDEAIGRALVGDYVVQEVVELPVEEFP
                     VFGESEWSMQPMYVDTNPFLFRGRVEGAMVRLSGSPVVNVTSGGGETGFFVIEGAAGE
                     "
     gene            complement(1794..2054)
                     /locus_tag="VF240_15245"
     CDS             complement(1794..2054)
                     /locus_tag="VF240_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176619.1"
                     /translation="MRLLFVAVLVFVINLPFGYWRSRVPARSRQWFLAIHLPVPAVIA
                     LRVLSGLGWQLASFPVLVGAFFLGQYVGGCLPRLFTRRPLPQ"
     gene            complement(2153..3775)
                     /locus_tag="VF240_15250"
     CDS             complement(2153..3775)
                     /locus_tag="VF240_15250"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX7176620.1"
                     /translation="MSEERQAAPVEAEEKQQPPPKSPSLLRNYLSLAGGAVAAASLTS
                     IVMLFLIELTSGSDNPYLGILTYIILPGFMLFGVVLVLLGMWRERRRRRRTPDAVPTF
                     PVLDLNDPRRRRNFIGLAGVVFVFIFMSAFGSYRAYEHTESVQFCGESCHVMKPELTA
                     FRVAPHANVRCVDCHVGPGPEWYVRSKLSGVRQLYHVTFGTYSRPIKSPVHNMRAATE
                     TCAHCHWPDKFHGDEMKEFKHFGYDEANSPRETRMLVHVGGGSPESGPVSGIHWHMNL
                     ANEITFVSTDEQRQDIAWVSWKDRAGNVVEYTARDSKLTPEQIAAAPKRRMDCIDCHN
                     RPTHIYQPPDRSVDDSLAAGKLDASLPFIKRESVALLSKSYATTDEAVHSIANGLEAF
                     YRDNYPDVYSGKSDAIRGAVSEVQRIFQTYTFPEMKTDWQTHPNNIGHFYAQGCFRCH
                     DGDHVSNTGLTISKDCRICHTTLDQTEGGVRTQAANGLFRHPVALGTQGEFQCAACHK
                     GNRGFEHPLNLGDISQFQCTDCHAGKVWTTSR"
     gene            complement(3990..4313)
                     /locus_tag="VF240_15255"
     CDS             complement(3990..4313)
                     /locus_tag="VF240_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7176621.1"
                     /translation="MYRIKMLAVALITLPLLVAALAGQSPTTASASPDDFDAAAVYKA
                     KCAMCHGQKAEKKFDATKADEPLVEIILKGKDDVTPKMPAYEAKGITAEQAKALTAHM
                     KSVKQ"
     gene            complement(4553..5470)
                     /locus_tag="VF240_15260"
     CDS             complement(4553..5470)
                     /locus_tag="VF240_15260"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7176622.1"
                     /translation="MHEPLEQHPSGPSATRASRWRRWLSLAAIASACLVFALTLLLGA
                     RASTAPAPSEVETSTGEIVTAESQADYSRFTHTNAQHARLPCALCHKRDDNSPRPVRS
                     VGHTPCSGCHTQQFADTSSPICTICHADPQSGAVKPFPTLRSFNVRFDHATHARGGAR
                     PASNCSACHRPERRGVALSIPISFGAHTTCFQCHTPGAQAGGQDISSCNTCHNLGGYR
                     RTPETAPAFRVNFSHAEHTRKGLGCNECHTVRAGAPQGRQVTSPQPLMHHASPRAQSC
                     MTCHNDRRAFGGDDFSDCKRCHEGNTWHF"
     gene            complement(5486..6736)
                     /locus_tag="VF240_15265"
     CDS             complement(5486..6736)
                     /locus_tag="VF240_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176623.1"
                     /translation="MKLRAATLIVFLSVVLLWLAPPRAASTIAAAPGVETEPQRRSRQ
                     GPRRAPAGRAPRIDYSNFDHATAQHQKACDSCHKFPSANWKQARQKEPFPDIAEYPEH
                     SSCLDCHRQQFFARERPQPKICSVCHVGATPRNTVRWPFPTLADTYDASPKGQRAASD
                     FGVSFPHDKHEGLFGELEIEGSREARYVRASFKTDAAPRQDDPAKQNAACATCHQTFQ
                     PQGDADDEYVTARPKTLAEDAFWLKKGTFKTSPRDHASCFTCHSTEGGIEPMPTNCAA
                     CHKLLPAAARLGLVSAHDDFDPKLAASMNVSDRATLRRWAGREVARFRHEWPPHDLAC
                     TACHKTAQMNAAEPQARRVPVLSCGGEGTGCHIEPTPDGLLNTAVARKLADPAFTCTS
                     CHVVNGRLLTPASHTAALPAATKK"
     gene            complement(6980..9250)
                     /locus_tag="VF240_15270"
     CDS             complement(6980..9250)
                     /locus_tag="VF240_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176624.1"
                     /translation="MRSKAKHAARTAAGLALCLALGAAAASAQQPSPSPNTAPPPSPS
                     PQKSADAQTQTTAPAESGEYAGPYRITSSVEIGVRGLSVGGDLNKYQSDLNYKAGPRL
                     FDASLLMQARQGEGEAFDTLLVTTTGWGSDPNGHVRVSAEKSRWYNFDASYRRFKYFR
                     FLNNIANPFYTTRPTDPATGQHGYDTRQQVGDFDLQLLPKNDRIRFNVGFSPTRYSGP
                     VFTTWHYGGDDFMLLSQAKYRSNDFRVGADWKLGPVDFSFQQGHRRFTEETAIDNDGI
                     NLGVNPAASNATLRSIERIQPVHGRVNYSRFSAHTLLAKKLDITGRLIYSSATTDFDW
                     TETVVANNFNTRITNIPGAINPPNLLPLGQFNFFGDAKRPNTLGDLGVTYLATDRFRL
                     SNTFRVETFQISGGTFYNGVFNLTRANGTGAVTLRPAGNAYEFTKYRKFQNTIEGDYE
                     FSERFSMRLGFRHGRRHIETFFRGANLASNGAPPLPDDAHEEDNQTNTVLFGFKARPV
                     RGWLLSLDAEHGTSDNVFTRTGNYNFTTIRARSRYAVRRNLRFNLGFIARENSNPSEI
                     DVNGSAVSLADFGVDIKSRVFTSSVDWTANDKLSFGAGYNYHWQNSDAVIEYAFGPGV
                     PNAGIRGHSLYFVRNHYFNFDVSAQPFRRVSVYAAYRVNRDAGQGNRLSNYSAGEFIS
                     SYPMHYQSPEARVSIRLNRRLDWNLGYQYYNYNESRLATYNQGTFIISPRPQNYHAHL
                     PYTSLRIYFGRSGDGR"
     gene            complement(9322..10341)
                     /locus_tag="VF240_15275"
     CDS             complement(9322..10341)
                     /locus_tag="VF240_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEX7176625.1"
                     /translation="MNRLRTIKLALVAAWVLGAVLLMGGTSLSRPTAEATQKPPPGQA
                     AQPGDYVGEDTCMQCHADQAEHYARTPHADLKNEPSWKGKVVGCESCHGPGKAHVEAG
                     GEKALIRHFENESPKQISDTCLSCHAGREEHNNYRRGEHWRNDVGCIDCHSSHSPTTG
                     ENRAGSNAFISGANAEKPGFAGEKMLKASEPQLCMQCHTETKHQFNKPFRHKVLEGAM
                     KCSDCHNAHGGFELKQARLATGADAACIKCHTDKQGPFTYEHAPVKTEGCASCHTPHG
                     SANPRLLRTSSVAQLCLECHTQDHGVGALEPVGPAHRLDLQYRDCTACHVKVHGSHTS
                     PVFMR"
     gene            11152..12672
                     /locus_tag="VF240_15280"
     CDS             11152..12672
                     /locus_tag="VF240_15280"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX7176626.1"
                     /translation="MSSIRSANASTSSKSSAAAVAPAVAPSSVIDRTMLLLSGVRFGV
                     GLLVALAAACMVGMLIMQANMEGFDKYYADLTPSQKLLYGKLGFFDIYHTWYFNALLL
                     VLSLNIVLSSIDNFPKAWTYLSRPKLDASPQWLGGQELHAALTVDGDDSQAVAMRVGA
                     ACSSLHYRTTITEEAGRHVVFAERGAWNRLGAYAVHVALLIIFVGGFLTAQLGHTGQV
                     ALAPGESANVMTETVFNLDQPLQVSVALPFEVECTDIQQKLIDKTGPTSPVNTLDWLT
                     RIRIKDPERGETEALVHMNAPFDYRGYRFFQSSFVAEGKARSIRLRVTPERPGAAAEE
                     VELMRDSTAQLADGAVIKFAGFFSDFVLNGSRGDSKSPEYVNPAAEIQVVDAAGVAAR
                     GFAFTSTAGAGPMVGRAFGGYKFELMDFEKVGAAHVLSVQKDPGATVVYVGFALLILT
                     LVGVFMFSHQRMWALVERGEGAHFQITLGGNTNRNRLGFEDRFKRLVKALNSTEAS"
BASE COUNT         1974 a         3952 c         4518 g         2327 t
ORIGIN      
        1 gacgacccgt tccaggtgct cgaccgcgaa ggcgtcggcc agctcgtcag gatgggcacg
       61 gagcgcgggc gcgcggcgaa gcccggcctc aaggtcggca tctgcggcga gcacggcggc
      121 gagccgcgct ccatcgcctt ctgccacgag acgggcctcg actacgtctc ctgctcgccc
      181 ttccgcgtcc ccgtcgcgcg cctcgccgcc gcacaggccg cgctcgcaga cagacgcgag
      241 gcggagacga cggacaagta atcaccgccg gcctataacg gaggtcgccg gaattaacat
      301 ggatgaacag atcagaaacg ggtgatgagt gatgagtgat gagtgataag aaagaaaaga
      361 agctgttagc tgttggttaa tcgctaagag ctaactgctt cttatcactt atcactcatc
      421 actcatcact tcttcatcct gcccatccat gttaattttc gtgcccatgc gcgaacgact
      481 gatcgctgag tatcacgaac tgctcaaggc ggacgagact ttgacgccgg agtttttcgc
      541 gcggctcaag ggcgcgatga cggtgcggcg gctggtctac ggcgagcgcc agataggagt
      601 ctcgctcagg ccccacctgc tcacccgcgc gcagtacgac gacctcgcgc gctcctcgga
      661 agtcctcgcc ggtgcgttcg agaaaatcac ggcggctcta atcgccaacc cttcaatgat
      721 gagcgaggtc ggcctcaacg agcgcgagca gagcctcgcg ctcgtcaacc ccggcttctc
      781 caactcgtcc gtgacgacac ggctcgacgc cttcatcggc cgcgagggtg tgaagttcgt
      841 cgagtacaac gccgagaacc cttcgagcct caccgaccag tcgggcctca accagattct
      901 cttcgaggtg cgcgcgcttc agaaaatctc cgggcgctac cgcctaaggc agttcgaccc
      961 cgcggaggcg ctgctcgacg cgctgctgga gaccttccgc gagtggggag gccgaagcgt
     1021 cgtgcccaac gtcgctatcg ttgactgggc gggcgtgccg accgaaggcg agttcatcct
     1081 gctgcgaaac tacttcgtcg gccgcggcgt gccgaccgtc atctgctcgc ccgaagagct
     1141 ggagtacgac ggcacgcgcc tgcgccgcgg cgactttccg gtagacctcg tttacaagcg
     1201 cgtcatcatc cacgaactgc tcgcgcggtg cgacgatatg cacccgctca tgcgcgcctg
     1261 ccgcgatggc cgcgtctgtc tggccaaccc cttccgctgc aagatcgcgc acaagaaggc
     1321 cgccttcgag ttgcttacgg atgacgagcg cgggggctgg ttcacgtccg aagagaggga
     1381 agtcgtccgc cgcacggtgc cgtggacgcg ccgcgtccgc gagcggaaga cttcgcaccg
     1441 ggggcgcgag gttgatttaa tcgagtacgt gcgccgcgag cgcgagttgt tcatcatcaa
     1501 gccgaatgac gattacggcg gccagggcat cacgctcggg ccgcgcgcgg gcgagcaaga
     1561 gtgggacgag gcgatagggc gtgcgctcgt gggcgactac gtcgtgcagg aggtggtgga
     1621 gctgccggtc gaggagttcc ccgtcttcgg cgaaagcgag tggtcgatgc agccgatgta
     1681 cgtggacacc aacccgttcc tcttccgcgg gcgcgtcgag ggcgcgatgg tgcgcctttc
     1741 cggctcgccc gtcgtcaacg tcacctcggg cggcggcgag acgggcttct tcgtcattga
     1801 gggagcggcc ggcgagtgaa gagtcgcggg agacagcctc cgacgtactg cccgaggaag
     1861 aaagcgccga cgaggacggg gaacgaggcg agctgccagc ccaatccgct caggacgcgc
     1921 agcgcgatga ccgcgggcac cggcaggtgt atggccagga accactgccg cgagcgcgcg
     1981 ggcacgcggg agcgccagta gccgaacggc aggttaatca caaagacaag tacggcgacg
     2041 aacagtagtc tcattgtcca cctttatgga gtgagcagta agcagtgagc agtcaaccac
     2101 catttcgcct tagctgctca cggcacaccg ctcactcctt tgctatccca cctcaacggc
     2161 tagtcgtcca caccttcccc gcgtggcagt cggtgcactg gaactgcgag atgtcgccga
     2221 ggttgagggg gtgttcgaag ccgcggttgc ccttgtgaca ggcggcgcac tggaactcgc
     2281 cctgcgtgcc gagcgcgacg gggtggcgga acaatccgtt cgccgcctgc gtcctgaccc
     2341 ctccctccgt ctggtcgagc gtggtgtgac agatgcggca gtccttgctg atcgtgagtc
     2401 ccgtattgct tacgtggtcg ccgtcgtggc agcggaagca cccttgggcg tagaagtggc
     2461 cgatgttgtt cggatgcgtc tgccagtcgg tcttcatttc ggggaaggta taggtctgga
     2521 agatgcgctg cacctccgag accgcgccgc ggatcgcatc actcttgccg gaatacacgt
     2581 cggggtagtt gtcgcggtag aacgcctcca gcccgttagc gatggaatga accgcctcgt
     2641 cggtcgtcgc gtaggacttc gagagcagcg cgaccgactc gcgcttgatg aagggaagag
     2701 aggcgtcgag cttcccggca gcaagcgagt cgtcaacgga gcggtcgggc ggctggtaaa
     2761 tatgcgtcgg gcggttgtgg cagtcgatgc agtccatccg gcgcttcggg gccgcggcga
     2821 tctgctccgg cgtgagcttg gagtcgcgcg cggtgtactc gacgacgttg cctgcgcggt
     2881 ctttccaact gacccaggcg atgtcctgcc gctgctcgtc cgtggagacg aaggtgatct
     2941 cgttggcgag gttcatgtgc cagtggatgc cggagacggg gccggactcg gggcttccgc
     3001 cgccgacgtg gacgagcatc cgggtctcgc gcggcgagtt ggcctcgtcg tagccgaagt
     3061 gcttgaactc cttcatctcg tcgccgtgga acttgtccgg ccagtggcag tgggcgcacg
     3121 tctcggtcgc ggcgcgcatg ttgtgcacgg ggcttttaat cggccgcgag taggtgccga
     3181 aggtgacgtg gtagagctgg cgcacgcccg agagcttcga gcgcacgtac cactccggcc
     3241 ccggcccgac gtggcaatcg acgcagcgga cgttcgcgtg cggcgcgacc ctgaaggcag
     3301 tcagctccgg cttcatcacg tgacagctct ctccgcagaa ctgcaccgac tcggtgtgct
     3361 cgtaggcgcg gtagctgccg aacgcgctca tgaagatgaa gacgaagacc acgccggcga
     3421 ggccgatgaa gtttcggcgc cggcgggggt cgtttaagtc gagcacgggg aatgtcggaa
     3481 ccgcgtcggg cgtgcgccgc cgcctgcgcc gctcgcgcca catccccagc aggacgagga
     3541 cgacgccgaa gagcatgaag ccggggagga tgatgtaagt gaggatgccc aggtaggggt
     3601 tgtccgaccc cgaggtcagc tcgatgagga acagcatcac gatgctggtg aggctcgcgg
     3661 cggcgaccgc cccgccggcg aggctcaggt agttgcggag caggctcggg gatttcggcg
     3721 gaggctgttg cttctcctcc gcctcgacgg gtgcagcttg tctctcctcg ctcatgtgcc
     3781 tccttcttcg cggtcgcggc cggcgctccc gatgcgcgcc ggacactctc gcccctgcta
     3841 tctgaatcct ttcggggccg ccacgcgaac ataaagggcc gcccgcgccg tcaccgctcg
     3901 ggcgggtgcg acgcgggcgg ccctttatgt tccatgtcgg cgcacgcgcc cggcggcgtg
     3961 tgccggccgc tccgcgcgct tcctcccggc tactgcttca cagacttcat gtgggcggtg
     4021 agcgccttcg cctgttcggc cgtgatgccc ttcgcctcgt aggcgggcat cttgggcgtc
     4081 acgtcgtctt tgcccttgag gatgatctcg acgagcggct cgtcggcctt cgtcgcgtcg
     4141 aacttcttct ccgccttctg gccgtggcac atcgcgcact tcgccttgta gacggccgcc
     4201 gcgtcgaagt cgtcgggcga ggcgctggcc gtcgtgggcg actgcccggc gagggccgcg
     4261 accagcagcg ggagggtgat gagggcgacc gcgagcatct ttattctgta catgattccg
     4321 tcttccttcc tcgaatctgt cggcggccgg cgccgcctgc cctcgtcggt gcaacgggcg
     4381 tgccggatgg ctgctgccgc gggagggcgt ttttccgggg ataaagacgg aactgcgacc
     4441 ccgccccgtc cgggcggcgt gctttcgcgc accttgatgg gtaactttcc gcaaggctcc
     4501 ggttctcatc agacggctaa gtcatggcac gccacgtccg taagctgata attcagaagt
     4561 gccacgtgtt cccctcgtgg cacctcttgc agtcggagaa gtcgtcgcct ccgaaggcac
     4621 gccggtcgtt gtgacatgtc atgcagctct gcgcgcgcgg gctcgcgtga tgcatgagcg
     4681 gctgcgggga cgtgacctgc cggccctgcg gcgcgcccgc cctcaccgtg tggcactcgt
     4741 tgcagcccag acccttgcgc gtgtgctcgg cgtggctgaa gttgacgcgg aaggccggcg
     4801 ccgtctcagg cgtccggcgg tagccgccga ggttgtggca ggtgttgcac gaagagatgt
     4861 cctgaccacc ggcttgcgcg cccggcgtgt ggcactggaa acatgttgtg tgcgcgccga
     4921 agctgatggg aatcgagagc gcgacgccgc ggcgctcggg ccggtgacag gccgagcagt
     4981 tcgacgcggg gcgcgcgccg ccgcgcgcgt gcgtcgcgtg gtcgaagcgg acgttgaagc
     5041 tgcgcagggt ggggaacggc ttgacggcgc ccgactgtgg gtcggcgtgg cagatcgtgc
     5101 agatcggact cgacgtgtcg gcgaactgct gcgtatggca ccccgagcag ggtgtgtggc
     5161 cgacggagcg cacggggcgc ggcgagttgt cgtcgcgctt gtggcacagg gcacacggca
     5221 gacgtgcgtg ctgcgcgttg gtgtgcgtga atctcgaata gtcggcctgc gactctgctg
     5281 tgacgatttc gcccgttgaa gtctcgacct ccgacggggc gggcgcggtg gaggcgcggg
     5341 cgccgagcag gagcgtgagt gcaaagacga ggcaggcgga ggcgatggcg gcgagcgaga
     5401 gccaacggcg ccagcgcgac gcgcgcgtcg ccgacgggcc ggacggatgt tgctcaagcg
     5461 gttcgtgcat gacagacatc gccgctcact tcttcgtcgc cgccgggagc gcggccgtgt
     5521 gtgacgccgg cgtgagcagc cgcccgttca cgacgtggca cgaggtgcag gtgaatgccg
     5581 ggtcggcgag cttgcgcgcg accgccgtgt tgagaagccc gtcgggcgtc ggctcgatgt
     5641 ggcagccggt gccctcgccg ccgcagctca gaacgggcac gcgccgggcc tgcggctcgg
     5701 cggcgttcat ctgcgccgtc ttgtggcagg ccgtgcaggc aaggtcgtgt ggcggccact
     5761 cgtggcggaa gcgcgcgacc tcacgtccgg cccagcgccg cagcgtcgcg cggtctgaga
     5821 cgttcatcga ggcggccagc ttcgggtcga agtcgtcgtg cgcgctcacg aggccgagcc
     5881 gcgcggccgc gggcaggagc ttgtgacacg cggcgcagtt ggtcggcatc ggctcgatgc
     5941 cgccttcggt cgagtggcac gtgaagcacg acgcgtggtc gcgcggtgag gttttgaacg
     6001 tgcccttctt gagccagaag gcgtcttcgg ccagagtctt cggccgcgcc gtcacgtact
     6061 cgtcgtcggc gtctccctgc ggttgaaagg tctgatgaca cgtcgcgcag gcggcgttct
     6121 gtttggccgg gtcgtcctgc cgcggcgcgg cgtcggtttt gaacgaggcc ctgacgtagc
     6181 gcgcctccct gctcccttca atttctaact cgccgaacag tccctcgtgc ttgtcgtgcg
     6241 ggaaggagac gccgaagtcg gaggcggccc gttgcccctt tggcgaggcg tcgtaagtgt
     6301 cggcgagcgt agggaagggc cagcgcacgg tgttgcgcgg ggtggcgccg acgtggcaga
     6361 ccgagcagat tttcggctgg ggccgctcgc gcgcgaagaa ctgctggcgg tggcagtcga
     6421 ggcaggagga atgttcgggg tattcggcga tgtcggggaa gggttccttc tgccgcgcct
     6481 gcttccagtt ggccgagggg aatttgtggc acgagtcgca ggccttctgg tgctgcgcgg
     6541 tggcgtggtc gaaattcgag taatcaatgc ggggcgcccg cccggcgggg gcgcggcgtg
     6601 gcccctgtcg tgagcgacgc tgcggctcgg tttcgacgcc gggcgcggcg gcaatggtcg
     6661 aggccgcgcg cgggggcgca agccacagga gcacgaccga aaggaagacg atcagcgtgg
     6721 cggcacgtag tttcatcggc tcacccggcg agtacgcggg gcattaaaac acagatgcca
     6781 cgacgagcaa taacacattc cgtggggcaa aaaacacacc cgcgtccgaa gacgccttcg
     6841 agagcggcgc ggccgccgca gcgagttcat cacgtgtggg cagactcggc cgcccctccg
     6901 tgtgggaggc ggccgggcct gcgttgagtt gtcaatcgtc aggggcgcgg gactgccgcg
     6961 cccctgacgc gcgtggttgc tacctgccgt cgccgctgcg gccgaagtag atgcggagcg
     7021 acgtgtaggg caggtgggcg tggtagttct ggggccgcgg gctgatgatg aaagtgccct
     7081 ggttgtatgt ggcgaggcgg ctctcgttgt agttgtagta ctggtagccg aggttccagt
     7141 cgagcctgcg attgaggcga atcgagacgc gcgcctcggg cgactggtag tgcatcgggt
     7201 aggaggagat gaactccccg gccgagtagt tcgagagccg gttgccctgc cccgcgtccc
     7261 tgttgacgcg gtaggccgcg tagacggaga cgcgtctgaa gggctgcgcc gacacgtcga
     7321 agttgaagta gtggttcctc acgaagtaga ggctgtggcc gcggatgccg gcgttgggca
     7381 cgccggggcc gaaggcgtac tcgatgacgg cgtcgctgtt ctgccagtgg tagttgtagc
     7441 cggcgccgaa tgagagctta tcgttggccg tccaatcgac ggacgaggtg aagaccctcg
     7501 acttgatgtc tacgccgaag tcggcgagcg agacggcgct gccgttaacg tcgatctcgg
     7561 aggggttgga gttctcccgc gcgatgaagc cgaggttgaa gcggaggttg cggcggacgg
     7621 cgtagcggct ccgcgcgcgg atggtcgtga agttgtagtt gcccgtgcgc gtaaagacgt
     7681 tgtcggaggt gccgtgctcg gcgtcgagcg agagcagcca gccgcgcacg ggccgggcct
     7741 tgaagccaaa gaggacggtg ttggtctggt tgtcctcctc gtgcgcgtcg tcgggcaggg
     7801 ggggcgcgcc gttgctggcg aggttggcgc cgcggaagaa ggtctcgatg tggcggcggc
     7861 cgtggcggaa gccgaggcgc atggagaagc gttcgctgaa ctcgtagtcg ccctcgatgg
     7921 tgttctgaaa cttccggtac ttggtgaact cgtaggcgtt gccggccggg cggagcgtga
     7981 cggcgccggt gccgttggcg cgcgtgaggt tgaagacgcc gttgtagaag gtgccgccgc
     8041 tgatctggaa ggtctcaacg cggaaggtgt tcgagaggcg gaagcggtcg gtggcgagat
     8101 aggtcacgcc taagtcgccg agggtgttgg gccgcttggc gtcgccgaag aaattgaact
     8161 ggccgagggg caagaggttc ggcgggttga tggcgccggg gatgttggtg atgcgggtgt
     8221 tgaagttgtt ggcgacgacc gtttctgtcc agtcgaagtc ggtcgtggcg ctgctgtaga
     8281 tgaggcggcc cgtgatgtcg agcttctttg cgagcagcgt gtgcgcgcta aaccggctgt
     8341 agttgacgcg gccgtggacg ggctggatgc gctcgatgct gcggagggtg gcgtttgagg
     8401 ccgccgggtt gacgccgagg tttatcccat cgttgtcgat ggcggtctct tcggtgaagc
     8461 ggcggtggcc ctgctggaag gagaaatcga cggggccgag cttccagtcc gccccgacgc
     8521 ggaaatcgtt ggagcggtac ttggcctgtg acagcagcat gaagtcgtcg ccgccgtagt
     8581 gccaggtggt gaagaccggg ccgctgtagc gcgtgggcga gaagccgacg ttgaagcgga
     8641 tgcggtcgtt cttcggcagc agttgcaggt cgaagtcgcc gacctgctgg cgggtgtcgt
     8701 agccgtgctg acccgtagcg gggtcggtcg gccgggtggt gtagaacggg ttggcgatgt
     8761 tgttgaggaa gcggaaatat ttgaagcggc ggtagctcgc gtcgaagttg taccacctgg
     8821 atttctccgc gctgacgcgg acgtggccgt tggggtcgga accccagccg gtggtcgtga
     8881 cgagcagggt gtcgaaggct tcgccctcgc cttggcgggc ctgcatgagc aggctcgcgt
     8941 cgaagaggcg cgggcctgcc ttgtagttga ggtcgctctg atacttattg aggtctccgc
     9001 cgacgctcag gccgcggacg ccgatctcca cgctggaggt gatgcggtag gggccggcat
     9061 attccccgct ctcggcgggg gctgttgttt gagtctgagc gtcggcggac ttctgcgggc
     9121 tgggtgacgg cggcggtgct gtgtttgggc ttgggctggg ctgctgcgcc gaggcggcgg
     9181 ccgcgccgag cgcgaggcac agcgccagcc cggccgccgt gcgcgccgcg tgtttagctt
     9241 tgcttctcat gtcgtccatc ctctcgctcg aacattctcg ttcgagactt cacggccgta
     9301 cttcggcggc ggcgaccgtc ctcaacgcat gaagacgggg ctggtgtgcg acccgtggac
     9361 tttgacgtgg caggccgtgc agtccctgta ttgcaggtcg agccggtggg ccgggccgac
     9421 tggctctaac gcgccgacgc cgtgatcctg cgtgtggcac tcaaggcaga gctgcgcgac
     9481 gctgctcgtc cgcagcagcc tcgggttggc cgagccgtgc ggcgtgtggc aggaggcgca
     9541 cccttcggtc ttgacgggcg cgtgctcgta tgtgaacggc ccctgcttgt ccgtgtggca
     9601 cttgatgcag gcggcgtccg cgcccgtggc gaggcgcgcc tgcttcagct cgaagccccc
     9661 gtgcgcgttg tggcagtcgg agcacttcat ggccccttcg agcaccttgt ggcggaacgg
     9721 cttgttgaac tggtgcttcg tctcggtgtg gcactgcatg cagagctgcg gctcgctcgc
     9781 cttcaacatc ttctcgccgg cgaagccggg cttctcggcg ttcgccccgg agatgaaggc
     9841 gttcgagccg gcgcggttct ctcccgtggt gggcgagtgc gacgagtggc agtcgatgca
     9901 gccgacgtcg ttgcgccagt gctcgccgcg ccggtagttg ttgtgctcct cgcggccggc
     9961 gtggcacgaa aggcaggtgt cggagatttg cttcggggac tcgttctcga agtggcggat
    10021 gagcgccttt tcgccgcccg cctcgacgtg cgccttccca gggccgtggc acgactcgca
    10081 gcccacgacc ttgcccttcc acgaaggctc gttcttgagg tcggcgtgcg gcgtgcgtgc
    10141 gtagtgctcg gcctggtcgg cgtgacactg catacaggtg tcttcgccaa cgtagtcgcc
    10201 gggctgcgcg gcctgacccg gcggcggctt ctgcgtggcc tcggccgtcg ggcgcgagag
    10261 cgacgtgccg cccatgagga gcacggcgcc cagaacccac gcggcgacca gcgccagctt
    10321 gatggttctc agcctattca tcttttgcct ccaaacttct cggccccggc cggggcctcc
    10381 gcatcgtccc tcgaaagggt cccagcgact gtcgttacgg cccggcgggg caacgcgggt
    10441 atgagtgctc aaagagcctg aaaaaagacg acggccgacg cggcgaggcg acggccgtca
    10501 cgaaaggtca gaagttgtcg tcgtcgttcg ccttctgccg gcgcaggcgc acggcgggcc
    10561 ggcggcggcg gccgtcttcg ccgcgaccac gctcgcccac acgtctgaga gtgacacatg
    10621 accctaccca tccgccgcgg gcggccccac ttcgaggccc gccccggcgg ccttcacgcc
    10681 agacgtgtgc aacgcacgtt ccgcgacgag ttggcgacca cagttgtcag ggatttcgcg
    10741 aggtggctgc gaggtgactg cgaggcgatt ttattcgata acctgccgca ggcaataact
    10801 ttttttgtta ctggatggtt gaacttttaa tgcgcgctcg ccagcgcgcg cgaccactca
    10861 cggtcggctg cggcgtgagg ataatttccc acggctgcga gaaatttccc gcgcgcggcg
    10921 cggccccggc cagctcgttg actgaagacg cgggggcgaa ttggcgtcgc gtgtcgagtc
    10981 gcgcgctggc acatcagttg ccgcttcggt gtgtgttgaa atcgggtcgc tcggtcgcgg
    11041 gggggtgatt gttcgctccg gcgcgcacgg cagatggccc tccacttcgt cggacgccgg
    11101 ggccagcccg aagtcccgac gagccacaca ctaaacgccg ggccagaaac gatgtcatcc
    11161 atcagaagcg caaacgcctc cacgtcgagt aagtcgagcg ccgccgccgt cgctcccgcc
    11221 gtcgccccct cctccgtcat cgaccgaacg atgctgctcc tgagcggcgt gcgcttcggc
    11281 gttgggcttc tcgtcgcact ggccgcggcc tgcatggtgg ggatgctcat catgcaggcc
    11341 aacatggagg ggttcgacaa gtattacgcc gatctcaccc cttcgcagaa actcctctac
    11401 ggcaaactcg gcttcttcga catctaccac acgtggtact tcaacgcgct cctgctcgtc
    11461 ctctccctca acattgtcct ctcatcaatc gacaacttcc cgaaggcgtg gacatatctc
    11521 tcccgcccta agctcgacgc gtctccccaa tggctcggcg ggcaggagct gcacgcggcg
    11581 ctgacagtcg atggcgacga ttcacaggcc gtcgccatgc gcgtcggcgc cgcgtgcagt
    11641 tcgttgcact acaggacgac gatcaccgag gaggcgggcc ggcacgtggt cttcgccgag
    11701 cgcggcgcgt ggaaccgtct gggcgcctac gccgtccacg tcgcgctgct catcatcttc
    11761 gtgggcggct tcctgacggc gcagctcggc cacacgggcc aggtggccct tgcccccggc
    11821 gagagcgcca acgtgatgac cgagaccgtc ttcaacctcg accagcccct acaggtgtcg
    11881 gtcgccctcc ccttcgaggt cgagtgcacg gacatccagc agaagctcat cgacaagacg
    11941 gggccgacct cgcccgtcaa cacgctcgac tggctgacgc gcatcaggat taaagacccc
    12001 gagcgcggcg agaccgaggc gctcgtccac atgaacgcgc ccttcgacta ccgcggctac
    12061 cgcttcttcc agtcgagctt cgtcgccgaa ggcaaggcgc gctcgatacg cctccgcgtc
    12121 acgcccgagc ggccgggcgc cgcggccgaa gaggtcgaac tcatgcgtga ctccacggcg
    12181 caactcgcgg acggggccgt cattaagttc gccggcttct tctccgactt cgttctgaac
    12241 ggctcacgcg gcgactcgaa gtcgcccgag tacgtgaacc cggccgccga aatccaggtc
    12301 gtggacgccg cgggcgtggc cgcgcgtggc ttcgccttca cttcgacagc gggcgcgggg
    12361 ccaatggtcg ggcgcgcgtt cggcggttac aagtttgagc tgatggactt cgagaaggtg
    12421 ggcgcggcgc acgtcctgtc ggtgcagaaa gaccccggcg ccacggtcgt ctacgtcggc
    12481 ttcgcgctgc tcatcctgac gctcgtcggc gtcttcatgt tctcgcacca acgcatgtgg
    12541 gcgctcgtcg agcgcggcga gggcgctcac ttccaaatca ctctgggcgg gaataccaac
    12601 cgcaaccggc tcgggttcga ggaccgcttc aagcggctag tcaaagccct gaattcgacg
    12661 gaggcttcat aagtcatgag tagagtcctg aaacaaacgc cggccgcgtt cgcgccggaa
    12721 atcggcttca aactgcccag tgcgtccgcc tacctgccga ccgtgttcgc c
//
LOCUS       DASVES010000373        13491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1225778, whole genome shotgun sequence.
ACCESSION   DASVES010000373 DASVES010000000
VERSION     DASVES010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13491
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225778"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..952)
                     /gene="larE"
                     /locus_tag="VF240_15285"
     CDS             complement(62..952)
                     /gene="larE"
                     /locus_tag="VF240_15285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX7176627.1"
                     /translation="MTETVVDLSIEDAARDGEAAAAGSALAKEDALRVWLRAAGSVLV
                     AFSGGVDSTYVALVANEELGERALCVTGDSASLSEHQREQVRELAARLRLNHLVVRTD
                     ELNDSDYLRNASDRCYYCKTELYEKLLPLASEHGLACVVDGSNADDLGDHRPGRAAAA
                     ERGVRSPLVEVGIRKQELRLLSRRAGLPTWDRPASPCLSSRIAYGTPVTIERLRAVET
                     GEDILRALGFREFRVRHHDELVRIEVAPAELDRALRREVADELARRFRALGFRYVTLD
                     LHGYRTGAMNEILKPVISDE"
     gene            1236..2705
                     /locus_tag="VF240_15290"
     CDS             1236..2705
                     /locus_tag="VF240_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176628.1"
                     /translation="MPSRTQQNAFTFVLGLLLTLFCCAAPALAQDEDFDEDAPDPIKL
                     LRRGQAAHARGVEKKSEEDLRAALEFYEQAIRLNPEFAEAEYQKGVVLGVLGRGAEAE
                     KSFRRAMEINPEWLMPPAALGQMLARTPGREREAEEVLRRALELDPMSGPLLFALAEL
                     RRLAGDTVGALELLKRATADGDPAPSAMWVARGEIEKASGDIPSALKSLSRAVSLDSA
                     SARARFKRAELYVEAKDFARAAQDLQALEEPAKADAGMALSVASLYARAGDKDAARRV
                     LDSLPEPARQLPDAKRMRASLEDVKCEATPEAREALERMLADEPNNATLNSCLGELYR
                     TSDPPRSLAYYKRAAELQPANVKYATGYGAALIQLRRFDEAATLLRRVVAAAPDDYVA
                     RANYATALYELKLYRQALDEYAWMSIARPELAVIHFFVGIAHDRLGEYEEALRAYETF
                     LARADARTNQLEIDKVNLRLPSLRNQIKRGEGVKKKGGR"
     gene            2702..3334
                     /locus_tag="VF240_15295"
     CDS             2702..3334
                     /locus_tag="VF240_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176629.1"
                     /translation="MNAASHSFHTRLPAPARVLLAALLCAACLSIAAHAQTVEPADSA
                     PPKKYVPAAMKSQLAAVRNMKDRVKLTLTLLDERLQAASGYTSSENFSAAGNELGIYQ
                     ALVDDVIHFLHLNGKDNNRTRDLFKRVELALRSHVTRLETIRRITPSEEAVHIRSCIE
                     FVREARGRALDAFYDDDSVMRLPSPRQRKETNDGSAKSDAPPTEKKPDQN"
     gene            3390..3941
                     /locus_tag="VF240_15300"
     CDS             3390..3941
                     /locus_tag="VF240_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176630.1"
                     /translation="MKRHHAPRRRPHALLLLALFASLLPARIQVASTAQREHLTPEEI
                     EQVRVNQTLDLRTGVFVKAVERRLAAIADPTAAEKLSAKELEQWGAVKGTRAQLLSDI
                     DRILEEAVTNIEDAAIHSAKSSLVPKAVRRLAEAAKVFLPQLTALNTSASDDAERGAI
                     ERALEKLQEIIDAAGKQPAEVKK"
     gene            4654..5229
                     /locus_tag="VF240_15305"
     CDS             4654..5229
                     /locus_tag="VF240_15305"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7176631.1"
                     /translation="MDSRYYPGVEADTVQLVRELRQLFDSNEAYEVQTMQVHSTAIIQ
                     ARKAGMLREMTGLSAALTIRITPEHGGTRVEIGMQKWFDKVAVAAVAFIISAGLLAAL
                     PAIGAYWQHKLTENAWKIIEAHIAHKAGGYVPPMPGRCGTCGSVNVSGSPFCSGCGAD
                     MRAVRTCNSCGSQVKDSAARFCNRCGKPLTG"
     gene            complement(5250..5492)
                     /locus_tag="VF240_15310"
     CDS             complement(5250..5492)
                     /locus_tag="VF240_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176632.1"
                     /translation="MLSEQSFRERECIHRAGGAEGGLYRGTAYVEHLQRLSSYEALQF
                     AGRVTPFFWSDAAQIVVWLCDECAAELRLRDYAAAS"
     gene            5665..6048
                     /locus_tag="VF240_15315"
     CDS             5665..6048
                     /locus_tag="VF240_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7176633.1"
                     /translation="MEETRRADVTILVVEDYEDTSLAMRLALEDLGYRILEASDGEQA
                     VRIALEERPDIILMDLSLPVLDGLDATGRIRQHPEMADTVIVAVTAHIEPDYRTKALA
                     AGCNAYVTKPIDFDWLNDLIGNLLS"
     gene            6335..7405
                     /locus_tag="VF240_15320"
     CDS             6335..7405
                     /locus_tag="VF240_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176634.1"
                     /translation="MNTKTKSKINPLSRIPRQQRLVMAVRGGAGAGKSHFIRSMSEAG
                     LGRLCIFDVERKARLLKGVGTQFDALEIHEPDELPEFIHWALEGEGREQNYGCFALDS
                     WAAYFSAKHSEMVAAVRERTGDPLAQPSAEELAADQMILQGVLRRLCMESGKSVVIVD
                     QIPAKGKEAKEDNEVGRVLPMTASGLEYFVDVMVEVSMQERDGEITRVFQVIKSNSPV
                     FEVGFEMTGNIQFKDFIEHMRRTQGAELSMDAPKPSPVPDIIEEKPAPLTVVQPQMTV
                     QELVAKAEKMGFKQSDVVMGAKVYHNQPNIYRLTDEQIIDLERRMTARAEKLRAADAP
                     EEGRQAAAAGDASSRTRNLKRA"
     gene            7763..8347
                     /locus_tag="VF240_15325"
     CDS             7763..8347
                     /locus_tag="VF240_15325"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX7176635.1"
                     /translation="MGSGRLEAFSDGVLAIIITIMVLELKVPHGADLASLGPLIPVFL
                     SYVLSFIYVGIYWNNHHHLLHAVRHVNGSILWANLHLLFWLSLIPFVTGWMGENHFAA
                     WPVAFYGVVLLLAAIAYFILARALISEHGRDSALAKALGKDFKGKVSVVFYAVAIPLS
                     FVNSWLACALYVLVAVMWLIPDRRIERALTEPTA"
     gene            8611..9006
                     /locus_tag="VF240_15330"
     CDS             8611..9006
                     /locus_tag="VF240_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176636.1"
                     /translation="MKHNLILTIASLLSILFMSFHHADDVVRGMAPGTFSNLVPVFFL
                     LVWLYGTLVLAGRRAGYVVILVASLLASGIPVVHMRGSGLVGGIAKSSGDFGEFFFVW
                     TLIAADVTALFSVILSAHALWRLRRGQSR"
     gene            complement(9123..10640)
                     /locus_tag="VF240_15335"
     CDS             complement(9123..10640)
                     /locus_tag="VF240_15335"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7176637.1"
                     /translation="MRNAIGAPLIAPLVAMLAMTAATGALAEDWPMFRGPNGSGVSVS
                     TGLPEVFGPEKNVVWKTPLPPGHSSPVLTRDRVFVTAYDKEKASSGQPSAVGAQGNEG
                     EKNHKLLVICLDRQTGKLLWQREVPRSREGRLQNVNNPASPSPVTDGTNVYVFFQEFG
                     LVSYDGEGRERWRRPLGPFNMFYGFGASPILVDDKVILPVDQDSPSSYLIAVDKTSGR
                     VRWKVDRPAVISGYSTPIIYQPKQGPKQIVVPESFQLSAYSVADGKRVWWVRGLACEM
                     KSIASSDGEYVYINGWGFPQNQPGEQISTIPLEEAWKRYDKNNDKLIAKSEVAGAEKM
                     DRMLKSAFEAFDMDRDEKLNVKDWEVFRGMMASENGLLAIRMGGQGDQTATAIRWRYQ
                     KPVPQVPSTLLYKGVLYMINDSGILLSFDPATGSVIKQGRLHGAIDKYFASPVAADGK
                     VFVVGQGGQVSVLKAAGDWEVLAVNELDDEVFATPAIADGRVYIRTRSALYSFGK"
     gene            11118..12197
                     /locus_tag="VF240_15340"
     CDS             11118..12197
                     /locus_tag="VF240_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176638.1"
                     /translation="MSACEKIKDRITIGLIIIGVFITGLLAPRTEAQKREEKKSQTIP
                     TIAFISSRFDPALKPPAWLLAAEIYLMNGDGTNVRRITHNTTGEGFPALSPDGKRIVF
                     ESNRLRKESDPINWASLFVMNVDGSGEASLVPGNSATWSRDSKSVAFHASASGKSKPI
                     TGLPGAASIDSDIFILNVDDFLSKKSPARNITNNPDAVDDDPDWSPDGKKIVFTSHAA
                     TDDQTNSATAEIYLIGANGKGKSKRLTNNTEEERAPDWSPDGKLIAYMCRKGEAAEGR
                     SIGTFEICVMNADGSGQKRITNNRVPELTVSWSPDGKQLVFHRAVGGVGQFQLFTINA
                     DGTGEKQLTFPPGFNGFPSWGRAKM"
     gene            12515..12811
                     /locus_tag="VF240_15345"
     CDS             12515..12811
                     /locus_tag="VF240_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX7176639.1"
                     /translation="MTYIVTEDCVNCKYTDCAAVCPVEAFHELSDRLYINPDTCIDCD
                     ACVPECPVEAIYADSTLPAHFAEWLELNAQAADYPILSGKIPALYASGCKGNPE"
     gene            12869..>13491
                     /gene="ygiD"
                     /locus_tag="VF240_15350"
     CDS             12869..>13491
                     /gene="ygiD"
                     /locus_tag="VF240_15350"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX7176640.1"
                     /translation="MAESMPAIFFGHGNPMNAVSRNEYTEGWAAIGREIPRPAAVLCV
                     SAHWYLPATLVTAQNAPRTIHDFGGFPRELFEVQYPAPGAPQLAARVRDLLAPVTVGL
                     DESWGLDHGAWSVLCHVFPDADVPVIQLAIDETQPASFHYELGRRLAPLRDEGVLIVG
                     SGNLVHNLHAYGWGRRNVEPFDWAVRFEKLARASLLAGDHAPLVAYES"
BASE COUNT         2694 a         4364 c         3954 g         2479 t
ORIGIN      
        1 ataagtgatg agtgataagt gataagtaag aagaaagcgg gtctgccttt cttatcactt
       61 atcactcatc acttatcact ggtttcagga tttcgttcat cgcccccgtg cggtagccgt
      121 gcaggtcgag cgtcacgtag cggaagccga gggcgcggaa gcggcgggcg agttcgtcgg
      181 ccacctcacg gcgcagggcg cggtcgagtt cggcgggcgc gacctcgatg cgtaccaact
      241 cgtcgtgatg tcggacgcga aactcgcgga agcccagagc gcgcaggatg tcttcgccgg
      301 tctccacggc gcgcaggcgc tcgatggtga cgggcgtgcc gtaggcgatg cgcgaggaga
      361 gacagggact cgcgggcctg tcccacgtcg gcagcccggc cctccggctt aacagacgca
      421 actcctgctt ccgtatcccg acctcgacga gcgggcttct gacgccgcgc tcggccgccg
      481 ccgcgcggcc cggccggtgg tcgcccaggt cgtcggcgtt cgatccgtcc accacacaag
      541 cgaggccgtg ctcggaggcc agcggcagaa gtttttcgta caactcggtc ttgcagtagt
      601 agcagcggtc ggatgcgttt cggaggtagt cggaatcgtt aagctcgtcg gtgcggacga
      661 cgaggtggtt gagccgcaga cgcgcggcca gctcgcgcac ctgttcgcgc tggtgctcgg
      721 agaggctcgc cgagtcgccc gtcacgcaga gggcgcgctc gcccaactcc tcgttggcga
      781 cgagggcgac atatgtactg tcaaccccgc cggagaaggc gacgaggacg gagccggccg
      841 cgcgcagcca gacgcgcaac gcgtcctcct tcgcaagcgc cgacccggcg gcggcggctt
      901 ccccgtccct cgcggcgtct tcgattgaaa ggtctacgac cgtctctgtc accaacaccc
      961 ctctcgtcgg gacactcttt cggccccgaa cagactccgc gcggcgccgc agcgatttct
     1021 caacctgagt tgttagagct ttacggcggc aaatgttagc gcagtggtcg gggccgcgca
     1081 acccacaagt ctctttcatg catccacacg agatgcgcgc ctgcgtagtt tggacgatgc
     1141 tttcgagcgc gtgctatatt caaccgcgga ccggacggac aatttcttct cccgcgaact
     1201 ccattcaaga tcgcttgaag gtgcagcttt tccagatgcc ttcccgcact caacaaaacg
     1261 cgttcacctt cgtcctcgga ctcctgctca cgctcttctg ctgcgccgcg cccgcgctcg
     1321 cccaggacga agacttcgac gaggacgcgc ccgaccccat caaacttctc cggcgcggtc
     1381 aggcggccca cgcgcgcggg gtggagaaaa aatcggagga agacctccgg gcggccctcg
     1441 agttttacga gcaggccatc cggctcaacc ccgagttcgc cgaggccgag tatcagaagg
     1501 gcgtcgtgct gggcgtgctc gggcgcggcg cggaggccga gaagtctttc cgccgcgcga
     1561 tggagatcaa ccccgagtgg ctgatgccgc cggcggcgct cggacagatg ctcgcgcgca
     1621 cgccgggccg cgagcgcgag gccgaagagg ttttgcggcg cgccctcgag ttagacccga
     1681 tgagcggccc gttgctcttc gcgctcgccg agttgcgccg gctcgcgggc gacactgtgg
     1741 gcgcgctcga actactcaag cgcgccaccg ccgacggtga cccggcgccg agcgcgatgt
     1801 gggtcgcgcg cggcgagata gagaaagcgt caggcgacat accgtcggcg ttaaagagtc
     1861 tgtcgcgcgc cgtgagcctc gattccgcca gcgcccgcgc gcgcttcaag cgcgccgagt
     1921 tgtacgtcga ggcaaaagac ttcgcgcgcg ccgcgcagga cttgcaggcg ctcgaagagc
     1981 cggcgaaggc tgacgccggc atggccctga gcgtggcctc gctctacgcg cgcgcgggcg
     2041 acaaggacgc ggcgcggcgc gtgctcgact cgctgccgga gccggcccga cagttgcccg
     2101 acgccaagcg catgcgcgcg tcgctcgaag atgttaaatg cgaggcgacg ccggaggcgc
     2161 gcgaggcgct ggagcgaatg ctcgccgacg aacccaacaa cgccacgctc aactcctgcc
     2221 tcggcgaact ctatcgcacg tctgacccgc cgcgctcgct cgcctactac aagcgcgcgg
     2281 ccgagcttca gccggcgaac gtcaagtacg ccaccggcta cggcgcggcg ctcatccagc
     2341 tccgccgctt cgacgaggcg gccacactgc tccggcgtgt cgtcgccgcc gcgcccgacg
     2401 actacgtggc tcgcgccaac tacgccacgg cgctctacga actcaagctc tacaggcaag
     2461 ccctcgacga gtatgcatgg atgtccatag cgcggcccga gctggccgtc atccacttct
     2521 tcgtcggcat cgcacacgac cgcctcggcg agtacgagga ggccctgcgc gcttacgaga
     2581 cgtttctcgc gcgcgccgac gcccgcacca accagctcga gatcgataag gtcaatctgc
     2641 gactgccctc tctgcgtaac cagatcaagc gcggcgaggg cgtcaagaag aagggtggac
     2701 gatgaacgcc gcgtcccaca gtttccacac gcgccttccg gcgcccgccc gcgtcctgct
     2761 cgcggcgctc ctgtgcgcgg cttgcctgag catcgccgcg cacgcccaga cggtcgagcc
     2821 ggccgacagc gcgccgccga agaaatacgt ccccgccgcg atgaagtcgc agctcgccgc
     2881 ggtcaggaac atgaaggatc gcgtcaagct cacgctcacg ctcctcgacg agcgactcca
     2941 ggcggcctcc ggctacacgt cgtccgaaaa cttcagcgcg gccgggaacg agctgggcat
     3001 ctatcaggcg ctcgtcgatg acgtcatcca cttcctccat ttgaacggca aagacaacaa
     3061 ccgcacgcgc gatctcttca agcgcgttga gctggctttg cgctcgcacg tcacgcgcct
     3121 ggagacgatc aggcgcatca ctccttcgga ggaggccgtt cacatcagga gttgcatcga
     3181 gttcgtgcgc gaggcacgcg gccgcgcgct cgacgccttc tacgatgacg actcggtcat
     3241 gcgtctgccg tccccacggc agcgcaaaga gacgaacgac gggagcgcca agagcgacgc
     3301 accgcccaca gagaagaagc ccgaccagaa ctgacgcgcg cgccccggcc gacgcccgcg
     3361 cagcgcgccg ggacgcttac acgaacgcca tgaagagaca ccacgcccca cgtcgcagac
     3421 cgcacgcgct ccttctgctc gcgctcttcg cttccctgct cccggcgcgc atacaagtcg
     3481 cttcgacggc gcagcgagag catctgacgc cggaagagat cgagcaggtg cgcgtcaacc
     3541 agacgctcga tctgcgcacg ggcgtcttcg tcaaggccgt cgagcggcgc ctcgcggcca
     3601 tcgccgaccc gacggccgcc gagaagctct cggccaaaga gctggagcag tggggcgccg
     3661 tcaaggggac gcgcgcgcaa ctcctctctg acatcgaccg catcctcgaa gaggccgtca
     3721 ccaacatcga ggacgcggcc atccacagcg cgaagagttc gctcgtcccg aaggccgtgc
     3781 gccggctcgc cgaggccgcc aaagtcttcc tcccgcagtt gacggccctg aacacgtccg
     3841 cgtccgacga cgccgagcgc ggcgccatcg aacgcgccct cgaaaagctg caagagataa
     3901 ttgacgccgc gggcaagcag ccggccgaag tgaaaaagtg agtgggcgtt gcgaagtcgt
     3961 gtgcgcgaag ctgcccgcac gcgcaccatt cgcaacggcc tcccggcatc gtctctccat
     4021 cgacctcacg tcgaagaacc tgcaccggga tgagcactca tctaactttt tctaaattat
     4081 ttccccttgc aaaattgttg cgttgacagc cctgtcgatt agggatatac aaccgctccc
     4141 tcgcaagggg tcactcgctt ccgttgacga aatcctcaca gataccgtca acctcacgct
     4201 aatgaaacat ggcggtataa acctttcgtg atgccgttga gcaacttcgc ctttaaccgg
     4261 cgtagtcgcc gcatccctac gtgccggcat cgcggaagtg agtgacgaaa aacgcttcgc
     4321 acggcgaaga acaaaaatgt cctcacccca aaacaatcac tagcgaattc cctgctagtt
     4381 ttgacgcccg caagtctaca gagacttgcg ggccttcttc tttactacaa tccaccccgc
     4441 cggaattcaa ccctgcggcg cccctccgcg ccctgcggcg cctccgcggt gaatctacat
     4501 cgctttcact caagtggaat ctcggctcca cgcgccctcc gcacgcgtgc gcgcgcttcg
     4561 cggttgaacc cctcaagctc tttcggcgta agatagtggg cgcctgttag agcgggcggg
     4621 cgagactgat gcgcgaaagg attttctaac tttatggaca gcagatatta tcccggcgtc
     4681 gaggccgaca ccgttcagct cgtgagagag ctacgccaac tcttcgactc taatgaggct
     4741 tacgaagtcc agaccatgca ggtccactcg accgccatca ttcaggcgcg caaggcgggc
     4801 atgttgcgcg agatgacggg cctgtccgcc gcgctcacca tccgcatcac gccggagcac
     4861 ggcggcacgc gcgtcgagat cggcatgcag aagtggttcg acaaggtggc cgtggcggcc
     4921 gtcgccttca tcatctcggc ggggctgctc gccgccctcc ccgccatcgg cgcctactgg
     4981 cagcacaagc tcaccgaaaa tgcctggaag ataatcgagg cccacatcgc gcacaaggcc
     5041 ggcggctacg tcccgccgat gcccggccgc tgcggcacct gcggctccgt caacgtctcc
     5101 ggctccccct tctgctccgg gtgcggcgca gacatgcgcg ccgtccgcac ctgcaactcc
     5161 tgcggctcac aggtcaaaga ctccgccgcg cgcttctgca accgctgcgg caagccgctc
     5221 accggctgac cctcccggcc gcggccccct caagaggccg cggcgtaatc acgcagacgc
     5281 aattcggccg cgcactcgtc gcacagccac acgacgatct gcgcggcgtc cgaccagaag
     5341 aagggcgtca cgcgcccggc gaactggagc gcctcgtagc tcgaaagcct ttgcaggtgc
     5401 tcgacgtaag cagtgccgcg gtagagtccg ccctccgcgc cgcccgcgcg gtgaatacac
     5461 tcacgctccc gaaacgattg ctcgctcaac ataagaatcc gttcctttgt cactgggaat
     5521 aatctaaggt gcgccgggag agttggcggg ccgacatctt ggcgcgggaa cataataaac
     5581 acgcggggcc ggcgcgcatc gttgcaaagt ggttacaaag agattatttt gctgcctcgc
     5641 ccttgaagga gttgaagccg gagcatggaa gagacacgac gcgccgacgt gacgatcctc
     5701 gtcgtcgagg attacgagga caccagcctg gcgatgaggc ttgcactcga agacctcggc
     5761 taccgcatcc ttgaggcgtc tgacggcgag caggccgtcc gcatcgccct tgaggagcga
     5821 cccgacatca tcctcatgga cctgagcctg cccgtcctcg acggactcga cgccacaggc
     5881 cgcatccgcc agcaccctga gatggccgac accgtcatcg tcgccgtcac cgcccacatc
     5941 gaacccgact accgcaccaa agccctcgcc gccggctgca acgcctacgt caccaagccc
     6001 atcgatttcg actggctcaa cgatctcatc ggcaacctcc tgtcttaaat caactgccag
     6061 tcgcgccgtg atagcacacg gattgaacgg ataagacgga tttacacgaa tttccgtgca
     6121 aatccgttaa aatccgtctt atccgtgttc ccttgcactt tttttcgttt catcgatgaa
     6181 attctgttcc attcctgccc gactcgtgtt atagtccccg ccgagttaaa ggtcactctc
     6241 aaggggagag ggctaaaccc gccgatttac agcggtcggc gctcgttgcc tgaaccacac
     6301 gccggaacgg ccatttcgag gaaagaggag atttatgaac acgaagacga aatcgaagat
     6361 caatccgctc agtcgtattc cgcggcaaca gcgtctggtg atggccgtgc gcggcggggc
     6421 gggcgcgggc aaaagccact tcatccgcag catgtccgag gcgggcttgg gccgcctctg
     6481 catcttcgac gtggagcgca aggcgcggct cctgaagggc gtcggcacgc agttcgacgc
     6541 gctcgaaatc cacgagccgg acgagctgcc cgagttcatc cactgggcgc tcgaaggcga
     6601 gggccgcgaa cagaactacg gctgcttcgc cctcgactcg tgggcggcct acttctccgc
     6661 caagcacagc gagatggtcg cggccgtgcg cgagcgcacc ggcgacccgc tcgcccagcc
     6721 ctcggccgaa gagctggcgg ccgaccagat gatcttgcag ggcgtgttgc gtcggctctg
     6781 tatggagagc ggcaagtccg tggtcatcgt cgatcagatt cccgcgaagg gtaaggaggc
     6841 gaaggaggac aacgaggtgg gccgggtgct gccgatgacg gcgagcggcc tcgaatattt
     6901 cgtggacgtg atggtcgaag tctccatgca ggagcgcgac ggcgaaatca cgcgcgtctt
     6961 tcaggtcatc aagtccaaca gccccgtctt cgaggtcggc ttcgagatga ccggcaacat
     7021 ccagttcaaa gacttcatcg agcacatgcg ccgcacgcag ggcgcggagc tgtcgatgga
     7081 cgcgccgaag ccctcgcccg tccccgacat catcgaggag aagccggcgc cgctcacggt
     7141 cgtgcagccg cagatgaccg tgcaggagct tgtggcgaag gccgagaaga tgggcttcaa
     7201 gcagtctgac gtggtcatgg gcgcgaaggt ctaccacaac cagccgaaca tctaccgcct
     7261 caccgacgag cagatcatcg acctcgaacg ccgcatgacg gcgcgcgccg aaaagcttcg
     7321 cgccgccgac gcgcccgagg aaggccgaca ggccgccgcc gccggcgacg cttcttcacg
     7381 cacgaggaat ctcaagcgcg cgtaagtctt ctcctgtgac ttgctgcccg ccgaacgcgt
     7441 gtgggccgac gtttgaacgt cggcccacac gcgttttcgt tctcatgacg gttgacaccg
     7501 gcgccccgcc tgtcatagaa gcacgggctg tgggccgctc aagatattct ccgaggtcgc
     7561 tcgctgcgag gggcctcggc ggcgggacgg aagattcggc ttttgccgcc ccattatgta
     7621 attagaccga accactcggc ttttcactct ctattgcgta gttatgccga atgggtcggc
     7681 ttttcacgcc ccacagtaga gttcggccga atcattcagc acaatatgcg ttaggcgtta
     7741 ccacgttaaa tggagattgt gaatgggcag tgggcgattg gaagcgttta gcgatggcgt
     7801 gctcgctatc atcatcacca tcatggtgct ggaattgaag gtgccgcacg gggccgacct
     7861 ggcctcttta ggcccgctga tccccgtatt cctgagctat gtgctcagtt ttatttacgt
     7921 cggcatctac tggaacaacc accaccacct gttacacgca gtccggcacg tcaatggcag
     7981 catcctgtgg gccaacctcc atctgttatt ttggctgtcc ctaatcccct tcgtcaccgg
     8041 ctggatgggc gagaaccact tcgccgcctg gcccgtcgcc ttttatggcg tcgtgttact
     8101 attggctgcg atagcctact tcatccttgc ccgcgcactc atttcagaac acggcaggga
     8161 ttcggcttta gcgaaggcgc tgggtaaaga cttcaaaggc aaggtgtcgg tggtgtttta
     8221 tgctgtggcg attccacttt cctttgtgaa ttcctggctg gcctgcgcgc tgtacgtctt
     8281 ggtggcggtc atgtggctca tccctgaccg gcgcattgag cgggcgctta ctgagccaac
     8341 agcgtgatta gccgcgtcac gcctaacaac ggcatgcagc ggacgcgcaa gatagcggcc
     8401 ctctcaagat gaggttgagc gcgcgctgct gatgccgggc gttagcccgc tcatcccggt
     8461 cgtatgtcaa cggattctgg tcgagtccat cagacagaga aaaggaaaat gtctatcaga
     8521 caaatgcctt atcccctcca atcgaatact gtcaaccgat catgtgcctt cactgagagg
     8581 cgcacgcaaa tctaacaagg tgaatatcaa atgaaacaca accttatctt aaccatcgcg
     8641 tctctgctct cgatcctctt catgtcgttt caccatgcgg acgacgtcgt ccgcgggatg
     8701 gccccgggaa cgttctcgaa cctcgttccg gtgttcttcc tgctcgtctg gctgtacgga
     8761 acgctggtgc tcgccggacg gcgagcgggg tacgtcgtca tcctcgtcgc gtcgctcctt
     8821 gcgtcgggca tccccgtcgt ccacatgagg gggtcgggac ttgtcggcgg catcgccaag
     8881 tccagcggag acttcggaga attcttcttc gtctggacgc tcatcgcggc cgacgtgacc
     8941 gcgctcttct ccgtcatcct ctcggcgcac gcactgtgga gactgcgacg gggccagtcc
     9001 cggtagtccg tcggccaccg caaattaacc gtgagcgtcg agttgaggcc accgcccaac
     9061 cctactgtgc agggcttctc gacgagcgtg accgagcacc gtggttccct tgccagcaag
     9121 cgttatttgc caaaactata aagcgcgctg cgagtcctga tgtaaacccg cccgtcggca
     9181 atcgctggtg tggcgaagac ctcgtcgtcc aactcgttga ccgcgagcac ctcccaatcg
     9241 ccggcggcct tcaacacaga cacctgacca ccctggccga ccacaaacac cttgccgtcg
     9301 gccgccaccg gcgacgcgaa gtatttatca atagccccat gcaggcggcc ctgcttgatg
     9361 acactgccgg tcgccggatc gaacgagagc agaatcccgc tgtcattgat catatacagg
     9421 acgcctttgt agagaagtgt tgaaggaacc tgcggcaccg gtttctgata gcgccaacga
     9481 atcgccgtcg cggtttgatc gccttgtcct cccattctga tggcgagcag gccattctct
     9541 gaggccatca tgccgcggaa cacttcccag tccttcacgt tcaacttctc gtcgcggtcc
     9601 atgtcgaacg cctcgaaggc ggatttcaac atcctgtcca tcttctccgc gcccgccacc
     9661 tccgacttcg cgattagctt gtcgttattc ttatcgtatc tcttccatgc ctcttcaagc
     9721 ggaatcgtgc tgatttgctc tccgggttga ttctgcggga agccccagcc gttgatgtac
     9781 acatattccc cgtcgctgct ggcaatcgat ttcatctcgc acgccagacc gcgcacccac
     9841 caaactcttt tgccatcggc gaccgagtaa gccgagagct ggaacgactc gggaacaaca
     9901 atctgcttcg gcccctgctt cggttggtaa ataatcggcg tcgagtaacc cgagatgacc
     9961 gcgggccggt caaccttcca acgcacgcgc ccgctcgttt tatcgaccgc gatcagatat
    10021 gagctgggac tgtcctgatc gacgggcagg atcactttgt catcaacgag aatgggagac
    10081 gccccgaatc cgtagaacat attgaacggc ccgagcgggc gccgccaacg ctcgcggcct
    10141 tcgccgtcgt aagacaccaa cccgaactcc tggaaaaaga catagacatt cgtgccgtcc
    10201 gtaacggggc tgggcgaggc aggattattg acgttctgca aacggccctc acgcgagcgc
    10261 ggcacttccc gctgccacag cagctttcct gtctgacggt caagacagat aaccaacagc
    10321 ttatgattct tctcgccttc gttcccctga gcgccgactg ctgatggctg accgctgctg
    10381 gccttttcct tgtcgtatgc agtgacgaac acgcgatctc gcgtcagcac cggcgaagaa
    10441 tggccgggag gcagcggcgt cttccaaacc acattcttct cagggccgaa cacttccggc
    10501 agcccggtcg agacggaaac gccgctcccg ttcgggccgc gaaacattgg ccagtcttcg
    10561 gcgagcgcac ctgtcgcagc cgtcatcgcg agcatcgcga ccagcggcgc gatcaatgga
    10621 gcaccgatgg cgttcctcaa cttcaacacc ttccggcgcg agtttgtcct gagagagcga
    10681 aaagttttgt ttagcacgcg aaccatcctt aagagagctt gcaaggcaag cgcgattcta
    10741 attcaagcgg ggcgcaaaag aaacgacgca cgccgcccag cttgattctg aagcagaggg
    10801 gttagccttc gagaaaggca aaatacacga cgaccgcagg tggccgcgcc tgttgcggtt
    10861 gacagcacgg cacagtctgt catagaagca agtgcggctg tttgcggcag tttcagcagg
    10921 ggccgggagt ttaaaaaccg gcccctcttt tttgtattcc gggcgaaatt gtttgtctga
    10981 gtttcaggtg acgctcgaag ttgtcacact cgggtcacac tcgatttttg tatgctatat
    11041 gtttaccact atagtctact caacagtgtg ccacagcgaa gcagtgagtg gtcaaagggg
    11101 taaaaggaat aataattatg agcgcctgcg agaaaatcaa ggatcgaatc actatcggct
    11161 taataattat cggagtcttt atcacaggac tactcgcgcc acgcacggag gcacaaaaac
    11221 gggaggaaaa gaaatcgcag acaataccga ccattgcttt cataagcagc cgtttcgacc
    11281 cggcgctcaa acccccggcg tggctactcg ccgccgagat ttatctgatg aatggagacg
    11341 gcacaaacgt ccggcgaatt acgcacaaca cgaccggaga gggattccct gcgctgtctc
    11401 ctgacggcaa gcggatagtt tttgaaagta accggctgcg taaagagtct gatccgatca
    11461 attgggcgag cctgtttgtg atgaatgttg atgggagcgg ggaggcttct ctggttcccg
    11521 gaaattcggc gacgtggtct cgggacagca aaagtgtagc ttttcacgcc tccgcgtcgg
    11581 gcaagagcaa accgataacg gggcttcccg gtgccgcctc aatcgacagc gacatcttca
    11641 tcttgaacgt cgatgatttt ctcagcaaga agtcaccggc aaggaacatc acgaacaacc
    11701 ccgacgccgt agacgacgac cctgattggt cgcccgacgg taagaagatc gtttttacga
    11761 gtcatgctgc caccgacgat caaacaaatt ccgctacagc cgagatttac ctcattggtg
    11821 cgaacggaaa gggaaagtcc aagcggctca caaacaacac cgaggaggaa agggcgcccg
    11881 actggtcgcc agacggtaag ttgattgcct atatgtgcag aaaaggcgag gctgccgagg
    11941 gacgctcaat cggaacgttc gagatatgcg tcatgaatgc cgacggcagc ggacaaaaac
    12001 gaattaccaa caaccgcgtc ccagagttga ccgtgtcgtg gtcacccgac gggaaacagc
    12061 tggtattcca cagggcggtc ggcggagtcg gacagttcca gctcttcacg ataaacgcgg
    12121 acggcaccgg cgaaaagcaa cttacgtttc cccccggatt taacggtttt ccgagctggg
    12181 gaagggcaaa gatgtaaaca aattaaaagc tgcatttaag gtagccttca agatacccgg
    12241 atgcacactc agtttacagc ctattgctga cggcgattag cgggcgatta aacaggcggc
    12301 acttctgaac acaatacgat agtgcgttac gaaagtcggt tttagaggcg gcgtttatat
    12361 gtaaggcacg tcgaacaagt cattggagcc gaccgcgagt tagcttgccg ctcatcgaaa
    12421 acttgtccgt tcgcggcgtt gcgcgcgcgg gcctgccata gaagcaaatc aataatcatt
    12481 tccccgcgag ggggtttaat tcaaaggagc cggcatgact tacatcgtca cggaagactg
    12541 cgtgaactgc aagtacacgg actgcgcggc cgtctgcccc gtcgaagcgt ttcacgagct
    12601 gagcgaccgc ctctacatca acccggacac ctgcatcgac tgcgacgcct gcgttcccga
    12661 atgccccgtc gaggccatct acgcggactc caccctgccc gcgcactttg ccgagtggct
    12721 cgaactcaac gcgcaggcgg ccgactaccc catcctcagc ggcaaaatcc ccgccctcta
    12781 cgcgtcgggt tgcaaaggca atcccgaata aagcgtgcgg ccgcgtgaga cgcgcggccc
    12841 ctgttggctc gtttatgagg aggcacgtat ggccgagtcg atgccggcga ttttcttcgg
    12901 gcacggcaat ccgatgaacg ccgtgtcgcg caacgagtac accgaagggt gggcggccat
    12961 cgggcgagag attccgcggc ctgcggccgt gctctgcgtg tccgcgcact ggtacttgcc
    13021 ggccacactc gtcacggccc agaacgcgcc gcggacgatc cacgacttcg gcggcttccc
    13081 ccgcgaactc ttcgaggtgc agtatcccgc gcccggcgca ccgcaactcg ccgcgcgcgt
    13141 ccgcgacctg cttgcgcccg tcaccgtcgg actggatgag agttggggct tagaccacgg
    13201 cgcgtggtcg gtgctctgtc acgtcttccc cgacgccgat gtgcccgtca tccagctcgc
    13261 catcgacgag acgcagccgg cgtcgttcca ctacgagctg gggagacggc tcgcgccgct
    13321 acgcgacgag ggcgtgctca tcgtggggag cggcaacctc gtccacaacc tgcacgccta
    13381 cgggtggggc cgccgtaacg tcgagccgtt cgactgggcg gtgcgtttcg agaaactggc
    13441 gcgcgcgagc cttctcgccg gcgaccacgc cccgctcgtc gcctacgaat c
//
LOCUS       DASVES010000374         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_61367, whole genome shotgun sequence.
ACCESSION   DASVES010000374 DASVES010000000
VERSION     DASVES010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61367"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1011
                     /locus_tag="VF240_15355"
     CDS             112..1011
                     /locus_tag="VF240_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176641.1"
                     /translation="MMPFKRASFPLALFVALCLVTLGVSCGRGGDDDEDDDGAVAETT
                     LPTYSPTGKEGTLQGTISFNGAAPAPGVISMDADSKCASSNPDPKTEDMVVADGKLQN
                     AFVYISEGKLTEGNKALSGLGFAAGQPAVLDQQGCQYRPHVIGVMTRQKVNITNSDQT
                     AHNVNVQATKNDKLNPSQPASSPPIVAEFKRPETLIPVKCNQHPWMKAYIGVLGHPFF
                     AVTGPDGSFEIKGLPPGAYTLVAWHEKLKEQKQQITVGPSETKSGLTFTFNAATASDE
                     LDGGSLRVMPALEIPFLGGQKHH"
     gene            1036..1971
                     /gene="cyoE"
                     /locus_tag="VF240_15360"
     CDS             1036..1971
                     /gene="cyoE"
                     /locus_tag="VF240_15360"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX7176642.1"
                     /translation="MKGLAIDIHTTAEVVEAAAVVVARPALRERLSAYFELTKPRITL
                     FIALTSAAGFLLGSVPGALDYARLFHALLAVSLLSSGIATLNQYMERDLDRLMRRTSS
                     RPLPSGRLEPAQALLFGIALTVTAEVYLALFVNLLSAVVGLFIAAGYLLCYTPLKTKT
                     TLSTFVGAFPGAAPPLIGWTAATGRLDLEAWVLFAILFLWQFPHFFAIAWMYREDYAR
                     AGVKMLPVVEPEGRMTGQQIVLWALLLVPVSLFPVAVGLSGKLYLYGALVLGLLYLAS
                     SVVAAFSSTRQDARRLLLASVLYLPLLFGLMVANR"
     gene            2111..>2918
                     /locus_tag="VF240_15365"
     CDS             2111..>2918
                     /locus_tag="VF240_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7176643.1"
                     /translation="MGRLLAIFIWVMTILSVAMFFNPRWWFPPAITEHGPAYDQQYLL
                     TIIVVGVAFASAQIALGYAVWRFRDTGDGTRAVYSHGNNRLEMIWTVITALIFIGVAL
                     LGQRVWAALHFNPAPAGSAQVRVLAQQFAWNFHYAGPDSRFGRTDPNLIDDSSSPPRG
                     NIGLDETDAAAKDDAVSSTLITQVDRHVELVLRGRDVTHSIWVPELRFKQDLVPGMDI
                     RVHFKPQKVGRYELACAELCGALHYQMKSYLLVLPEQQYAELMQLPQGEFT"
BASE COUNT          492 a         1029 c          847 g          550 t
ORIGIN      
        1 gcgtgcgttc gtccggcgtg gccgccttcc gcaatgccgg tcgcggcagt ttttcgccct
       61 gagagtttcg cgctatgatg agcacacaca tcagtcgatc ccggaggagt aatgatgccg
      121 tttaaacgcg cgagctttcc ccttgcactc ttcgtcgccc tctgtctcgt cacgctcggc
      181 gtctcctgcg ggcggggcgg cgacgacgac gaagacgacg acggagcggt cgccgaaacg
      241 accttgccca cctacagccc caccggcaag gagggcacgc ttcaggggac gatcagtttc
      301 aacggcgcgg cgccggcacc gggcgtcatc tcgatggacg ccgacagcaa gtgcgcgtcg
      361 tcgaatcccg accctaagac ggaagacatg gtcgtcgccg acggcaaact ccagaacgcg
      421 ttcgtctaca tcagcgaggg taagttgacc gagggcaaca aggcgctcag cggcctcggc
      481 ttcgccgcgg gccagccggc cgtgctcgat cagcaggggt gccagtatcg cccgcacgtc
      541 atcggcgtga tgacgcgcca gaaggtcaac atcacgaaca gcgaccagac ggcgcacaac
      601 gtcaacgtgc aggcgacgaa gaacgacaag ctcaacccgt cgcagccggc gagttcgccg
      661 cccatcgtgg ccgagttcaa gcggcccgag acgctcatcc ccgtcaagtg caaccagcac
      721 ccgtggatga aggcttacat cggcgtgctc ggccacccgt tcttcgccgt caccggcccc
      781 gacggctcct tcgagatcaa gggcctgccg ccgggcgcct acaccctcgt cgcctggcac
      841 gagaagctga aagagcagaa gcagcaaatc accgtcggcc cgagtgagac caagtcggga
      901 ctgaccttta cgttcaacgc cgccacggcc tccgacgaac tcgacggcgg cagcttgcgc
      961 gtcatgcccg ccctggagat tccattcctc ggcggccaga agcatcactg acgacggcgc
     1021 tttattgaga accgaatgaa gggtctggcg atagacattc acacgaccgc cgaggtcgtc
     1081 gaggcggccg cggtggtggt cgcgcgtccc gccttgcgcg aacgtctctc ggcctacttc
     1141 gagctgacca agccgcgcat cactcttttt atcgcactca cctcggccgc cggctttctc
     1201 ctcggctccg tgcccggcgc actcgactac gcgcgtctct tccatgcgct cctcgccgtg
     1261 tcgctcctct cgtccggcat cgcaacgctc aatcagtaca tggagcgcga cctcgaccgg
     1321 ctgatgcggc gaacttcttc gcggccgttg ccgtcgggcc gtctggagcc ggcgcaggcg
     1381 ctcctcttcg gcatcgcgct caccgtcacg gcggaagttt atctcgcgct cttcgtcaac
     1441 ctgctctcgg ccgtcgtcgg cctcttcatc gcggcgggct acctgctctg ttacacgcct
     1501 ctgaagacga agacgacgct ttcgaccttc gtcggcgctt tccccggagc cgccccgccg
     1561 ctcatcggct ggacggccgc cacggggcgg ctcgacctcg aagcatgggt gctctttgcc
     1621 atcctcttcc tgtggcagtt cccacacttc ttcgccatcg cctggatgta ccgggaagac
     1681 tacgctcgcg cgggcgtgaa aatgctgccc gtcgtcgaac cggaggggcg catgaccgga
     1741 cagcagatcg tcttatgggc gctgctgctc gtacccgtca gcctgttccc cgtggccgtc
     1801 ggactatcgg gtaagctcta cctttacggc gcgctcgtgc tcggcctcct gtatctggcc
     1861 agcagcgttg ttgccgcgtt ctcgtcaacc cggcaagacg cgcgccggct gctcctggcc
     1921 tccgtgctct atctgccgct cctgttcgga ctgatggttg ctaacaggtg ataagtgatg
     1981 agtgataagt gataagaagc tgttagcttt tagctgttgg ctgttagcta atcgctaaga
     2041 gctaacagct aacagccccc tttctttctt atcactcatc actcatcact catcacttct
     2101 ggagattgat atgggtcgcc tgctggccat cttcatctgg gtaatgacga tcctctcggt
     2161 ggcgatgttc ttcaacccgc gctggtggtt cccgcccgcc atcaccgagc acggcccggc
     2221 ctacgaccaa cagtatctcc tgacgatcat cgtcgtcggc gtcgccttcg cctccgcgca
     2281 gatcgccctc ggctacgccg tctggcgctt ccgcgacacc ggcgacggca cgcgcgccgt
     2341 ctactcccac ggcaacaatc gtctggagat gatctggacg gtcatcaccg ccctcatctt
     2401 catcggcgtc gccctcctcg gccagcgcgt gtgggccgcc ctgcacttca accctgcgcc
     2461 ggccggctcg gcacaggtca gggtgctcgc gcaacagttc gcgtggaact tccactacgc
     2521 cggcccggac agccgcttcg gccgcaccga ccccaacctc atcgacgact cctcctcgcc
     2581 accgcgcggc aacatcggcc tcgacgagac ggacgccgcc gcgaaggacg acgccgtcag
     2641 ctccacgctc atcacgcagg tggacaggca cgtcgagctt gtgctgcgcg ggcgcgacgt
     2701 gactcacagc atctgggtgc ccgagttgcg cttcaagcaa gacctcgtcc ccggcatgga
     2761 catccgcgtc cacttcaagc cgcagaaggt gggtcgctac gagctggcct gcgccgagtt
     2821 gtgcggcgcg ctccactacc agatgaagag ctacctgctc gttctgcctg agcagcagta
     2881 cgccgagctg atgcagctcc cgcagggcga gttcaccc
//
LOCUS       DASVES010000375        20596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_83165, whole genome shotgun sequence.
ACCESSION   DASVES010000375 DASVES010000000
VERSION     DASVES010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20596
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83165"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF240_15370"
     CDS             complement(<1..388)
                     /locus_tag="VF240_15370"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes a two-step reaction, first charging a
                     glycine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX7176644.1"
                     /translation="MPAPNIETIVSLSKRRGFVFPSSDIYGGLGSAWDYGPLGVELCN
                     NVKQAWWRWMVHERDDMEGIDSSIILNRAVWKYSGHEDTFSDPLVDCRDCNARLRADK
                     LEFDAMANAHVEAAIRAKDVKCPNCGS"
     gene            complement(617..778)
                     /locus_tag="VF240_15375"
     CDS             complement(617..778)
                     /locus_tag="VF240_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176645.1"
                     /translation="MAEGEGGGGNAGVVAIVVIFVIVLLVLFFGWGRFFGGGGNKDIN
                     IKVDTPATK"
     gene            complement(1029..2075)
                     /locus_tag="VF240_15380"
     CDS             complement(1029..2075)
                     /locus_tag="VF240_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176646.1"
                     /translation="MLTRSRMTRDLLPRVARRALAVAALLAVFASAAPLTPEAHAQQQ
                     QKKNGKPPTEGVIEISTEPGGYPIFIDDVYSGETTENMRVFKQTPGPHKVKIVFPNGP
                     PWEHTVNTTAGRKDCIRLTYRPRTPLPPPEVSFCPYSVNVTATATARDGDQITFTADA
                     AYAGQKSDLTYTWTVDPPTATITRGDRTPIITVDSTGIGNRRITATVVVEDSSGDPAC
                     RQQNRAETRFIPPPPPPPPYLDRFVSVAPDYDKARLDYLGDEMQRNPTYRAFLIAYGG
                     CGRRDRSPEVLLKRAYSYLVGERKMDANRITGIIGGYREHNGYDLHVQSPGENDPRPS
                     PTRGPCIAGRSTRR"
     gene            complement(2081..2755)
                     /locus_tag="VF240_15385"
     CDS             complement(2081..2755)
                     /locus_tag="VF240_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7176647.1"
                     /translation="MKHQAPRILALMLSGLLAAAAPARAGTIHFADVMQAAAAGRRGP
                     WADLRLRALQQQGGKTASGLVATTEQKQDAGASTGNAAGASDGSTTGNNPTSLISQDP
                     AQQPQGTVQTIDLGDISGTVCDCGEAPLEDVILKGGGFPWLSLVGIPLICLTGICTGG
                     EEVCVDCGTTSPTPNPTPPPPPPGIPEPATLLLFGSGLLALGAGSRRRRALKALEVEA
                     RDQEVV"
     gene            complement(3058..5343)
                     /gene="pnp"
                     /locus_tag="VF240_15390"
     CDS             complement(3058..5343)
                     /gene="pnp"
                     /locus_tag="VF240_15390"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7176648.1"
                     /translation="MTARKYLKESIKLGERELTVETGRVAKQAHGSVVIQYGETILLV
                     AAVGADKPREGIDFFPLTVEYREHNYAAGRIPGNYFRREGRPGEKEVLTARMIDRPCR
                     PLFTEGYKNETQVIASVISADDENDPDVIAITGASCALYLSDIPFPNPIAGVRVGLID
                     GRYIINPTYDEVRESRLNLIVAGTEEAITMVEAGAKGVSEEIMVEALMLAHKEINRLC
                     RWQKELYKALGVEKRTVEPPALNEEMLGEVERNYAEKMRQALDTSEQGKIASYAGIDS
                     LKKEVVESYPEDQPEQRQMAKKVFDHLKEKIFRDDILNKRRRPDGRRFSEIRGIDCEV
                     GWLPRVHGSALFTRGETQAIVTSTLGTKQDEQFMDDLESGEIKRRFLLHYNFPHFSVG
                     EVGRFGSTSRREIGHGALARRALEPVLPTVEEFPYSLRIVSDITESNGSSSMASVCGG
                     SLSMMDAGVPIKAAVAGVAMGLVMEGNKYAILSDIAGAEDHYGDMDFKVAGTADGITA
                     LQMDIKIAGVNAQIMAEALEQARKGRLHILERMNEALSESREDLSPHAPRIITIKINP
                     DRIRDVIGPGGKIIRAIVEETGAKIDVEDDGTIFIATADGDAAQAAVAKIRGLTAEAE
                     IGEEYLGTVSRIVDFGAFVEIFPGTDGLLHISEIAEHRVRDVRDELKEGQQIMVKCIG
                     KEGNKIKLSRRAVLRQEKQKAAGAGGGDGDFEGGGDVEVEDQGGGDSPGGDDSGGGND
                     RGENGRGGGGGGRGEGGRRRR"
     gene            complement(5788..6057)
                     /gene="rpsO"
                     /locus_tag="VF240_15395"
     CDS             complement(5788..6057)
                     /gene="rpsO"
                     /locus_tag="VF240_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7176649.1"
                     /translation="MSLSREVKESIVQEYRAKEGDTGSPQVQVALLTRRINDLTEHFK
                     THKKDNHSRRGLLKMVSQRRSMLDYLKRIDIEQYHELIGRLGLRR"
     gene            complement(6232..8748)
                     /locus_tag="VF240_15400"
     CDS             complement(6232..8748)
                     /locus_tag="VF240_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7176650.1"
                     /translation="MCRARLRELFRLLVVSAGASVSSAAALGTPLAQFDYRFLLLAAA
                     TLLLSSRVAVRIPRTGGRITVSDTGIFLTMLLCGGEQAVLLATADGAFSTLRISRKPL
                     TILFNAGVMACSTFLTVGLLRLCFGDEAVGAAGGQYSAPMAAVCLMACVQYAANSWLV
                     AIDAALRVGESLWRTWKESYLWTSVTYFAGAWAAHLTARLVSEYGFHSVIAATPIVAI
                     VYFTYRTYLKSLEATAAQAAQAERHVGELSRYIAEQERISLALQESEQRFRSAFNHAA
                     GTALVSPEGRWLQVNHSLCRMLGFTEQELLATDFQAVTHPEDLAEDLASTRRLLEECD
                     AIRPAEKRYLHRDGREVWVLQSASVVRDQCGAPLHLIFQIQDITDRKRNEELVRFAAY
                     HDALTGLPNRALLTDRLTRAIARARRSADYQHAVLFLDLDRFKVVNDSLGHTLGDALL
                     VQLSRRLEACVRDDDSVARLGGDEFAILLDGIRDAGVPIRVAERIKAALARPFDLGGH
                     EIYTTASIGIAYSTADYLSSDDMLRDADTAMYRAKSAGKARHEIFDTLMHSSAVELLR
                     LENDLRRALDRGELRVFYQPIVMLATGRIVGFEALSRWQHPERGLIPPAEFIPLAEET
                     GLIVPLGLHVLREACRQMRVWQDEFPSARSLSVSVNLSARQLSQPDLVHCVRQALEDS
                     GLAPYCLKLEITETVLMENAVSAIEMLMQLRRLGVQLSVDDFGTGYSSLSYLHRLPID
                     ALKIDRSFVGRMDEDGESTGIVEIIMMLASKLNKMAVAEGVETQEQARQLTALGCELG
                     QGYFFSPPVCAESAAGLLRGVVVGPAGLAEGVQEALSSVN"
     gene            complement(8827..10800)
                     /locus_tag="VF240_15405"
     CDS             complement(8827..10800)
                     /locus_tag="VF240_15405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX7176651.1"
                     /translation="MRDSKRRSLAAWFVSLFLLVAPWSGIVSGDRAGAQSLLGGLTQT
                     VQSVTQIIKVSPDLLSLVLGGRVGERVDVIVQTNGPAGLTLDLLLGVLGGLVKADYRN
                     LNARAVNIPVASVLQLAASLDVAYVSPDRGVSVLGHVSATTGADAARTAGAAVPLDGT
                     GVGIAVIDSGIDAAHKSFLDRGNATRVVFGRDFTGEGRTDDPYGHGTHVAALVAGNGR
                     ISNGAYTGVAPNARLVNLRVLDSRGTGRVSGLLGALDWLLANRTAYNIRVVNMSLGAP
                     AVDSYKYDPVCRAVRKLVDKGVVVFAAAGNEGKDGAGQKTYGRIHTPGNEPSAVTVGA
                     ANTFGTDARSDDAVATYSSRGPTRSYWIEKGGTRHYDNLVKPDLVAPGNKLTSAAAEG
                     NLLVTSHPELDAGVSPAAGRRMMTMSGTSMATPVAAAAAALMLQANPKLTPNLVKALL
                     MYTSQPLAGYNAFEQGAGLLNVEGAVRLARLVRTDLSSSTPLGAPLLVATAPPSPATS
                     IAGQSFSWSQGIVLNYTYVTGLGLITKYQKFYGSGALLGDGVIAGDAVITRNLSMMSD
                     GIQIGEAIMTSSGVIAGDGYVFCAYGVLLGQGKLLDDGEVFGDGVIAGDGVIAGDGVI
                     AGDGVIAGDTLLGGDHTSWMQALVDDGTDFFAY"
     gene            11293..12537
                     /locus_tag="VF240_15410"
     CDS             11293..12537
                     /locus_tag="VF240_15410"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7176652.1"
                     /translation="MDAKDDKDPERARIVVVGGGYGGLFTAMQLERAGAVTLVTTEPN
                     FLHTPLLYEYMSGEVEAWHIAPAYEELLGERIRFVQGAVTNVDFEAREVEVAGRVRTL
                     SYDYLVLAVGGVTNFWGVEGADKHAMPFRKIRHADDLRARMIEALDSIQPDAPPQDAR
                     RAATFVVVGAGASGVELSTKMSDLLYDAFSRRGLKGEPHVVVVEMGREAVPGMDAEIR
                     RVVERALYERRIEIHTETRVVKVTPDGAVIEHAGERQEIKASGVVWTAGVRTNPLVEK
                     LDVERDRRGLIVVEPSMQVKGRPGVFALGDIAAYTDVPPSLFGTAQLAFQESDLVAAN
                     VQALIDGGVPSRGHFKELGEAVSLGTADAAVLASGQVVTGQLARQARFALYTTRLPTW
                     QHRLKVGAAWFFGGKTPRPLGL"
     gene            12774..13430
                     /locus_tag="VF240_15415"
     CDS             12774..13430
                     /locus_tag="VF240_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7176653.1"
                     /translation="MNEQTEDRRDFLKRASMAVIALPFLISCKTDTLAQKTENELSLI
                     KKNAMPSGTEARGAIDVPDNVSWKTSLSKKSDKDEPMIISGTIFQPDGRTPAPNILIY
                     FYHTDSDGYYGRRGEVRDGHFRGWMLTGANGKYEFSSIKPAPYPNRTEAAHVHMTLTG
                     RKFKEDSINAILFEGDKFITTEERNSVGKWGEPNPILKLEKGADGILRGTRDFKLWKV
                     "
     gene            13696..14304
                     /locus_tag="VF240_15420"
     CDS             13696..14304
                     /locus_tag="VF240_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7176654.1"
                     /translation="MIFNTLSVGSGQSTTTDGHRIVPIRQMSQDVEILYGDPEKVGEP
                     FVMRIRELPGGVIPPHKHPVDEHITVVQGTLYFGVGDKFDRAALKELKAGSYAFIPKG
                     STMFGYTPEAAIVQVHGVGPFHIHWRAGTNWRDSLKTLDSPDAGTVFKFRRGERVTTK
                     RGRGRIRQGYDSGEIIGYEIDGEDGRLFMAEEGEIKRLGDKK"
     gene            complement(14649..15770)
                     /locus_tag="VF240_15425"
     CDS             complement(14649..15770)
                     /locus_tag="VF240_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7176655.1"
                     /translation="MSDRDDDDRKPNYDLTTPNIYPPQQNPRPPARTPYQSGGDFDRT
                     SVNIPTQGEGGRRQPPPPPSTGSGNKYDLTMVNIHGPLGADEDDEAAYARVSSQTAGQ
                     QHAPPYTAQYPTADYQQPPPQPAQPAEAQQRSAPVWARVAGWGLLAFSLLLAGGFVAY
                     LLWPSSGFTLKVLNAPHGSKVFVDDIPMGVSQQESGILTHGLRPGEAREVRVAHEGYV
                     DWRTTVEGRRGETTVLRVKMTPLPKTESALPEADDQFEQDLEEFGRVRIYSITFDPGS
                     DVIRGESKPPLDRIVGVLKKRTDWALTIEGHTDSTATAQYNQELSQRRAAAVKSYLQT
                     GGIDAARLSTVGYGASKPVSGNDTPVGRALNRRVEFIKK"
     gene            complement(15789..16880)
                     /gene="dusB"
                     /locus_tag="VF240_15430"
     CDS             complement(15789..16880)
                     /gene="dusB"
                     /locus_tag="VF240_15430"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX7176656.1"
                     /translation="MKPFKIRDVEINPPLVLSPMAGVTDVPFRALLKRRGGIGLTVSE
                     FISVEGLTRNNPKSKRQMRFFDYERPFAVQIFGGRPERMRMAAELAEEVGADILDINC
                     GCPAPKVVKHGGGSGLLRDLPRLETILKDIKKAISIPLTIKIRAGYYDYNINALETAR
                     LAEACGVEHIALHGRTKEQGYKGRANWDLVRQIKEVVSVPVSGSGDILTVEDAFARWR
                     ETNCDGLLIGRGAMANPWIFRQIEDAAAGREPFEPTLEDKRAVLLEYFELLRADMPEM
                     AAVGRMKQLAGQFTRGLPGGARFRQVIYHSHSVSEILERIGDYFETIRAGLFYAGEGD
                     PPEGESEPPALDSCEAATEELRGHHDRAA"
     gene            complement(16964..17506)
                     /locus_tag="VF240_15435"
     CDS             complement(16964..17506)
                     /locus_tag="VF240_15435"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HEX7176657.1"
                     /translation="MKTALATLFLAAGLASATAVTAQHTQHSTDDKGGKQDKQTTAAA
                     EKAADDREHIRRGEKIGDSPTVPFAHVLKEPQKYAGKRVVVEGVVERVCKSEGCWMQI
                     APEAGAAESVRVTFDHKFSVPKDADKMRFRAEGEFSVKVLTKEEVEHLVKDDGAKIKT
                     NPDGTANEVSFLATGVELWK"
     gene            17596..18096
                     /locus_tag="VF240_15440"
     CDS             17596..18096
                     /locus_tag="VF240_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176658.1"
                     /translation="MRGRLIQSIGIAWTLLFGAAVVWLYTAQPQSVADVATKASVTAG
                     TYEVDRARAQAAQALFAREQYRAARDEWVRADPERSDARTQFYVAYAFYREGWGRVGS
                     DDALFRQGLEAVNLAISLSPGQTLRVDDPELKIQTAAELKAEIESGLELTAADFNPLK
                     VFRERK"
     gene            18116..18889
                     /locus_tag="VF240_15445"
     CDS             18116..18889
                     /locus_tag="VF240_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176659.1"
                     /translation="MTDNRSRNRALVEFLLLPTIFLTVALLGGLRVSAGTGAFVFVVP
                     PLVTLICALLLLALAARGRLVRLERWLSGDNRPLTNVAHALTLAALFFASAQAFNSVL
                     PEAGLLRWMFSFFFLWTLWNNQFALFDARRLLRSLAVLFGTAFLLKHLLLAGLFAPGG
                     SLARRLVGGLLEGVTLGAIDAPAYAPATGYISFFTLLLYVAGLALLPSAPTVDDAPEG
                     ADALLAQAADDRALPAGGEAVFETPAIEERRARVIDMDE"
     gene            19068..20312
                     /locus_tag="VF240_15450"
     CDS             19068..20312
                     /locus_tag="VF240_15450"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7176660.1"
                     /translation="MMEQQANGIEDPGASLARAASRGELAQVRLLLDAGADPRAKDRH
                     GSTALFYAAGAGHYEIARLLLEGGADPEASNVVGMTPHAVATSRGHAQLAALIESHKT
                     GGRSGGVEGALDRGRPALLRAAREGDAAGVRRLLEAGADADTRDESAWTPLMLAAVGG
                     HTEAVGMLLAGGAQPNARNDKGWTPLMLAVSLGDAGLARALLDAGADPNVRDNEGLTP
                     LMQAVGENNVECARLLLERGADAGLTNHSGETAQSIAARHAFPEVLQLFGETETHNAD
                     AATPAFDLTSPEQLFRTEWVGEPDTEPSARDAEPGGAAVPAEVEATSESQSLVPAQSA
                     PESVERLIMALEAMRQQRPAAPLADIAHKLMLTLQEAAALTGLSQRRLREAIRAGQLK
                     AQIIGRGWRIKRTDLEDYVRDL"
BASE COUNT         3422 a         6519 c         6930 g         3725 t
ORIGIN      
        1 tcgagccgca gttagggcat ttaacgtcct ttgctcgaat cgctgcttcg acgtgagcgt
       61 tagccatagc gtcgaattcc agcttgtcag ctcgcagccg cgcgttgcaa tcgcggcagt
      121 ccacaagcgg gtcgctgaac gtgtcctcgt ggccggaata cttccagacc gcgcggttga
      181 ggatgatgct tgagtcgatg ccctccatgt cgtcgcgctc gtgaaccatc caacgccacc
      241 atgcctgctt gacgttgttg cagagttcga cgccgagcgg gccgtagtcc cacgcgctcc
      301 ccaagccgcc gtagatatcc gaggagggga agacgaagcc gcggcgcttc gagagcgaga
      361 cgatggtttc gatgttgggc gccggcacgt ttttctccgt gaatcgtcaa tcgtcaatcg
      421 aaaatcgtgg tcacgcttag gctcaactta agctacaaag cgcaggtcgg atgacggtca
      481 acccgccgat ttacgattga cgatttacga ttgacgagaa taacaggcgc gcgcgggaaa
      541 tgaaaaggag aggcgagcga cgcgcctcgg cgggcgcgcc gtccctctcc tctcgcgggc
      601 cgatgccctg cccggtctac ttggtcgcgg gcgtatcgac cttgatgttg atgtccttgt
      661 tgccgccgcc gccgaagaat ctgccccatc cgaagaacag gacgagcagg acgattacga
      721 agatcaccac gatggccacg acaccggcat tgccgccgcc gccttcacct tctgccatgt
      781 tatctcctcc tagtgatagg tcaaacgcgc ctttacttgc cccggcgcgc gggcctggct
      841 acgggagccg gcccttcgat taagcgatcc gcctgaaaag tcttttcgca cgcgcctcac
      901 cccggccttg ttaccacacg tctccgagtc gcgtcaacgc ccctcgaaac aagcgccgtg
      961 cccctcgcgg cccgaggggc acggcgcttt gactgacatt cgcaaagacg ccccgcctgg
     1021 cccttaggct agcggcgcgt gctcctgccc gcgatacacg gccccctggt cggactcggg
     1081 cgcgggtcgt tctcgcccgg cgactgcacg tgcagatcgt acccgttgtg ctcacggtag
     1141 ccgccgatga tcccggtgat gcggttggcg tccatcttgc gctctcccac gaggtaggag
     1201 taggcgcgct tcaggagtac ttcgggcgaa cggtcgcggc gcccgcagcc gccgtaggcg
     1261 atgaggaagg cgcggtaggt cgggttgcgc tgcatctcgt cgcccaggta gtcgagccgt
     1321 gccttatcgt agtcgggggc gaccgagacg aagcggtcta agtagggcgg aggcgggggc
     1381 gggggcggga tgaagcgcgt ctcggcccga ttctgctgac ggcaggcggg gtcgccggag
     1441 gagtcctcaa cgacgactgt ggccgtgatg cgtctgttgc cgatgcccgt ggaatcgacc
     1501 gtgatgatcg gcgtgcggtc gccgcgcgtg atggtggccg tgggcgggtc aaccgtccag
     1561 gtgtaggtga gatcagactt ctggcccgcg tatgccgcgt cggccgtgaa ggtgatctgg
     1621 tcgccatctc tggcggtggc cgtcgccgtg acgttcacgg agtacgggca gaatgacacc
     1681 tccggggggg gaagaggggt gcgcggccgg taggtgagcc tgatgcagtc cttgcgcccc
     1741 gcggtggtgt tgaccgtgtg ctcccagggc gggccgttcg ggaagacgat cttaactttg
     1801 tgcgggccgg gcgtctgttt gaagacgcgc atgttttcgg tcgtctcgcc ggagtaaacg
     1861 tcgtcgatga agatggggta gccgcccggc tcggtcgaga tttcgatgac gccctccgtc
     1921 gggggcttgc cgttcttctt ctgctgctgc tgcgcgtggg cctctggcgt gagcggcgcg
     1981 gcggaagcga agacggcgag cagcgcggcg acggcgaggg cgcgacgcgc cacgcgcggc
     2041 aagaggtctc gcgtcatgcg gctccgtgtc aacatgatct tcagacaacc tcctgatcgc
     2101 gtgcttccac ttcgagcgcc ttgagggcgc ggcggcggcg ggagccggcg ccgagcgcga
     2161 gcaggccgga gccgaagagc aggagcgtgg ccggctcggg gatgcccggc ggcgggggcg
     2221 gcggggtcgg gttcggggtc ggactagtcg tcccgcagtc tacgcaaacc tcctcgccgc
     2281 cggtgcagat gccggtcagg cagatgagcg ggatgccgac gagggaaagc caggggaagc
     2341 cgcccccctt gaggatcaca tcctcgaggg gcgcctcccc gcagtcgcag accgtgccgg
     2401 agatgtcgcc gaggtcgatg gtctgaaccg tgccctgcgg ctgctgggcc gggtcctggc
     2461 tgatgagcga cgtggggttg ttgccggtgg tggagccgtc ggacgcgccc gcagcgttac
     2521 cggtgctggc gcccgcgtcc tgcttctgct cggtggtggc gacgaggccg gaagctgtct
     2581 tgccgccctg ctgctggagg gcgcggaggc gaaggtctgc ccaggggccg cggcgacccg
     2641 cggcggccgc ctgcatcacg tcggcgaagt gtatggtgcc ggcccgtgcc ggcgccgccg
     2701 ctgcgagcag gccgctgagc atcagcgcga ggatgcgtgg ggcttgatgc ttcatcttga
     2761 gaggtcgcct cctgaaatcc ccggctcggc cggaaacttc aatggaaagt tctataaaga
     2821 cagatgcgcc ggccccggtc cgtcttccgc ttaatgttcg ggcccgcgcg gtcaattgtt
     2881 tacggcttga ccactcgtcc gacttgctca aagggaaaag acgcggaata gcttgttaat
     2941 tccaatttcg attgtcaaat taaactcgcg gggcgctcaa ctgtcaagga aaaaagaggc
     3001 ccgcccgcgg gggcgaacct cttccgtgtc aaaacttggc cgaaagcggc gggtgagcta
     3061 gcgccgccgc cgtccaccct cgccacggcc accaccacca ccgccacgac cgttctcgcc
     3121 gcggtcgttg ccgccgccgc tatcgtcgcc gccggggctg tcgccgccgc cctgatcttc
     3181 gacctcgaca tcgccgccgc cctcgaagtc gccgtcgccg ccgccggcgc ccgccgcctt
     3241 ctgtttctcc tgccggagca cggccctgcg ggagagcttg atcttgttgc cctccttgcc
     3301 gatgcacttg accatgatct gctgcccctc tttgagttcg tcgcgcacgt cgcggacgcg
     3361 gtgctcggcg atctcggaga tgtgcagcag gccgtcggtg ccggggaaga tttcgacgaa
     3421 ggcgccgaaa tcgacgatgc gcgagacggt gccgaggtac tcctcgccga tctcggcctc
     3481 ggccgtgagt ccgcgaatct tggccacggc cgcctgtgcg gcgtcgccgt cggcggtggc
     3541 gatgaagatc gtgccgtcgt cctcaacgtc gatcttggcg ccggtctcct cgacgatggc
     3601 gcggatgatc ttgccgcccg gtccgatcac gtcgcggatg cggtcggggt tgatcttgat
     3661 agtgatgata cgcggcgcgt gcgggctcaa gtcctcgcgc gactcgctga gggcctcgtt
     3721 catgcgctca aggatgtgca gtctgccttt acgggcctgc tccagcgcct cggccatgat
     3781 ctgcgcgttg acgccggcga tcttgatgtc catctggagg gccgtgatgc cgtcggccgt
     3841 gccggccacc ttgaagtcca tgtcgccgta gtggtcttcg gcgcccgcga tgtcggagag
     3901 gatggcgtac ttgttgccct ccatcaccag acccatcgcc acgcccgcga ccgccgcctt
     3961 gatggggacg cccgcgtcca tcatcgagag actgccgccg cagacgctcg ccatcgacga
     4021 ggagccgttc gactccgtga tgtcggagac gatgcgcagc gagtacggga actcctcgac
     4081 cgtgggcagg acgggttcga gcgcgcgacg cgcaagcgcc ccgtggccga tctcgcggcg
     4141 ggaggttgag ccgaagcgtc cgacctcgcc gaccgagaag tgcgggaagt tatagtggag
     4201 caggaagcgg cgtttgattt cgccggattc gaggtcatcc atgaactgct cgtcctgctt
     4261 ggtgccgagc gtcgaggtga cgatggcctg cgtctcgccg cgcgtaaaca gcgccgagcc
     4321 gtggacgcgc gggagccagc cgacctcgca gtcgatgccg cgaatctcgg agaagcgtcg
     4381 gccgtcggga cgacggcgct tgttgagaat gtcgtcgcgg aaaatcttct ccttcaggtg
     4441 gtcgaagacc ttcttggcca tctgccgctg ctcgggctga tcctcaggat aggattcgac
     4501 gacctccttc ttgagcgagt cgatgccggc gtaagaggcg atcttaccct gctcggaggt
     4561 gtccagagcc tgacgcatct tttcggcgta gttgcgctca acctcgccga gcatctcctc
     4621 gttcaaggcg ggcggctcga ccgtgcgctt ctcgacgccg agcgccttgt agagttcctt
     4681 ctgccagcga cagagacggt tgatctcctt gtgcgcgagc atcagggcct cgaccatgat
     4741 ctcctcggag acgcccttgg ccccggcctc gaccatcgtg atggcttcct cggtgccggc
     4801 gacgatgaga ttgaggcgcg actcgcgcac ctcgtcgtag gtcggattga tgatgtagcg
     4861 gccatcgatg agtccgacgc ggacgccggc aatcgggttc gggaacggga tgtcggagag
     4921 gtagagggcg cacgacgcgc cggtgatggc gatcacgtcc gggtcgttct cgtcgtcggc
     4981 cgaaatcacc gaggcgatga cctgcgtctc gttcttgtag ccttcggtga agagcgggcg
     5041 gcaggggcgg tcgatcatgc gggcggtcag gacttccttc tcgccggggc ggccctcgcg
     5101 ccggaagtag ttgccgggga tgcgccccgc cgcgtagttg tgctcgcggt actcgaccgt
     5161 gagcgggaag aaatcgatgc cttcgcgcgg cttgtcggcc ccgacggcgg cgaccaagag
     5221 tatcgtctcg ccgtactgga tgacgaccga gccgtgcgcc tgtttggcga cgcggcccgt
     5281 ctcgacggtg agttcgcgct cgccgagctt gatggattct ttcaaatact ttctggctgt
     5341 catgtgtttt cgtctcctta cgggcgcgac gcccgcgccg cgctggcggc ctcgggccgg
     5401 ggcgtctcgt ccgccccgca agcgccctgt taaattttta ttcgtggccg gcggccccgg
     5461 cgcggcgcgc tacgtgtggc gcgtcgcgtg aacgtttcgg tcgcgtgacc gtgcggcgga
     5521 aaaagaagac ggcaccgggc ttctggtgtc gtgaagcccg gtgccgcttg tctctagacg
     5581 atccgccggc gtggccgctc tcctggggcg tcggcccggc cgggaggtct cgcgccgtca
     5641 cgttcggcac cgcccgcggg cttttctgtt ggacgcctcg gagtgatgtt ttttacaggg
     5701 gggcgcctgg ttgccgtgcg ccccccgcgc gttcaagcta acccgaacgg cgcgactgag
     5761 ctttcaggag tgggccgcta gagaggtcta gcggcgcagg cccagtcggc cgatgagttc
     5821 gtggtactgc tcgatgtcga tccgcttgag atagtcgagc atacttctgc gctgcgagac
     5881 catcttgagc aggccgcgcc gcgagtggtt gtccttcttg tgggtcttga aatgctcggt
     5941 aaggtcgttg atgcgccgcg tgagcaacgc cacctgaacc tgcggcgacc cggtgtcgcc
     6001 ttccttcgcg cgatattcct ggacgattga ttctttgact tccctcgata acgacatact
     6061 gttcgagatg atcctcccag accgcagtca agcccgtctg tatccacagg caaccgcaag
     6121 agttagtaaa acttacccgg agcaatctag catgcggccc cgaggagtgt ccagccgggg
     6181 ccgcacgccc gatgtccgcc cctaattcct gctccggccc gccccaagct ctcaattgac
     6241 gctcgacagc gcctcctgca ccccttcggc cagccccgcc ggccccacca ccaccccgcg
     6301 gagcaggccc gcggccgact cggcgcagac cgggggcgag aagaagtagc cctgtcccag
     6361 ctcgcagccg agggctgtca gttgccgcgc ctgctcctgc gtctccaccc cctcggcgac
     6421 ggccatcttg ttgagcttgg aggcgagcat catgatgatc tcgacgatcc ctgtgctctc
     6481 cccgtcctcg tccatgcggc cgacgaagga gcggtcgatc ttcagggcgt cgatggggag
     6541 gcggtggagg taggagaggc tggagtagcc ggtgccgaag tcgtctacgc tcaactggac
     6601 gccgagtcgc cgcaactgca tgagcatctc gatggcgctc acggcgttct ccatcaggac
     6661 ggtctcggtg atctccagct tcagacagta aggcgcgagg cccgagtcct ccagggcttg
     6721 cctgacgcag tggaccagat cgggttgcga gagctgccgg gccgagaggt tcacgctgac
     6781 gctcaacgag cgcgccgagg ggaattcatc ctgccagacg cgcatctgcc ggcacgcctc
     6841 gcgcaggacg tgcagcccga gcgggacgat gaggccggtc tcctcggcca gcgggatgaa
     6901 ttcggccggc gggatcagcc cgcgctccgg gtgctgccag cgcgagagcg cctcgaaccc
     6961 gacgatgcgc cccgtggcca gcatgacaat cggctggtag aagacgcgca gctccccgcg
     7021 gtcgagcgcg cgccgcaggt cgttctccag cctgaggagt tcgacggcgc tggaatgcat
     7081 cagcgtgtcg aagatttcgt gccgggcctt gccggccgac ttggcgcggt acatggcggt
     7141 gtccgcgtcg cgcagcatgt cgtcggagct gagatagtca gcggtcgagt aggcgatgcc
     7201 gattgaggcg gtggtgtaaa tctcgtgccc gccgaggtcg aacgggcgcg cgagcgccgc
     7261 cttgatgcgc tcggccacgc ggatggggac gcccgcgtcg cggatgccgt cgagcaggat
     7321 ggcgaactcg tcgccgccga ggcgagcgac cgagtcgtcg tcgcgcacgc acgcctccag
     7381 gcggcgggag agttgcacca gcagcgcgtc gccgagcgtg tggcccaagc tatcgttgac
     7441 gaccttgaag cggtcgaggt cgaggaagag cacggcgtgc tggtaatcgg cggagcggcg
     7501 cgcgcgcgcg atggcgcggg tcaggcggtc ggtgaggagc gcgcggttgg gcagccccgt
     7561 gagcgcgtcg tggtaggccg cgaagcggac cagctcctcg ttgcgcttgc ggtcggtgat
     7621 gtcctgaatc tggaagatga ggtggagggg ggcgccgcac tggtcgcgga cgaccgaggc
     7681 gctctggagc acccagacct cgcgcccgtc ccgatgcagg taacgcttct cggccgggcg
     7741 gatcgcgtcg cactcttcga gcagccggcg cgtgctcgcc aaatcttcgg ccaggtcttc
     7801 ggggtgcgtg accgcctgga agtcggtcgc caacagctcc tgctccgtga agcccaacat
     7861 ccggcagagg ctgtggttga cctggagcca gcggccttcc ggcgagacca gcgccgtccc
     7921 ggccgcgtgg ttgaaggcgc tgcggaaccg ctgctcactc tcctggaggg cgaggctgat
     7981 gcgctcctgc tcggcgatgt agcgcgacag ctcgccgacg tggcgctcgg cctgcgcggc
     8041 ctgcgcggcc gtcgcctcca agctcttcag gtaggtccgg taggtgaagt agacgatggc
     8101 gacgatgggg gtcgcggcga tgaccgagtg gaagccgtac tcgctcacga gacgggccgt
     8161 gaggtgggcg gcccaggcgc cggcgaagta ggtgaccgaa gtccagaggt aggactcctt
     8221 ccacgtgcgc cagaggctct cgccgaccct gagcgccgcg tcgatggcga cgagccacga
     8281 attggcggcg tactgcacgc aagccatcag gcagacggcg gccatcggcg ccgagtactg
     8341 gccgcccgcg gcgccgacgg cctcgtcccc gaagcagagg cggagcaggc cgacggtgag
     8401 gaaggtcgag caggccatca cgcccgcgtt aaagagtatg gtgagcggtt tgcggctgat
     8461 gcgcagggtg gagaaggcgc cgtcggccgt cgccagcagc acggcctgct cgccgccgca
     8521 gagcagcatc gtgaggaaga tgcccgtgtc ggagacggtg atccggccgc ccgtgcgcgg
     8581 aattctgacg gcgacgcgcg agctgaggag gagcgtggcc gcggcgagga gcaggaagcg
     8641 gtagtcgaac tgcgcgagcg gcgtgccgag cgccgcggcg gacgagaccg aggcgcccgc
     8701 cgacaccacc aacagcctga acagctctct gaggcgtgct ctacacatga ctttcggact
     8761 gcggcgcggg ggttagccgc gcgggatggt cagtgggttc cggtgaggag tccggggccg
     8821 tgagagttaa tacgcgaaga agtcagttcc gtcgtccacc agcgcctgca tccaggacgt
     8881 gtggtcgccg cccagcagcg tgtcgccggc gatcacgccg tcgcccgcaa tcactccgtc
     8941 tccggcgatg accccgtcgc cggcgatcac gccgtcgccg aagacttcgc cgtcgtcgag
     9001 cagcttgccc tggccgagca gcacgccgta ggcgcagaag acgtagccgt cgcccgcgat
     9061 gacgccgctg ctggtcatga ttgcttcgcc gatctgaatg ccgtcggaca tcatggacag
     9121 gttgcgtgtg atgacggcgt cgccggcgat cacaccgtcg ccgagcagcg ccccgctgcc
     9181 gtagaacttc tggtacttcg tgatgaggcc gaggccggtc acgtaggtgt agttgagcac
     9241 gatcccctgc gaccaactga agctctggcc ggcgattgaa gtggccgggg agggcggcgc
     9301 ggtggccacg aggagcggcg cgccgagcgg cgtgctcgac gagaggtcgg tgcggacgag
     9361 ccgggcgagg cgcaccgcgc cttcgacgtt cagaagcccc gcgccctgct cgaacgcgtt
     9421 gtagccggcg agcggctgcg acgtgtacat cagcagagcc ttgacgaggt tcggcgtcaa
     9481 tttcgggttg gcctggagca tcagcgcggc ggctgcggcg gcgacgggcg tcgccatcga
     9541 agtgccgctc atcgtcatca tccttcggcc ggcggccggg ctgacgcccg cgtccagttc
     9601 cgggtggctc gtgacgagga ggttcccttc cgccgcggcg ctcgtgagct tattgccggg
     9661 cgcgacgagg tcgggcttga cgaggttgtc gtagtggcgc gtgccgccct tctcgatcca
     9721 gtagctgcgg gtaggcccgc gcgagctgta ggtggcgacc gcgtcgtccg agcgcgcgtc
     9781 ggtgccgaag gtgttcgcgg cgccgaccgt gacggccgac ggctcgttgc ccggcgtgtg
     9841 aatccgcccg taggtctttt gccccgcgcc gtccttgccc tcgttacccg ccgcggcgaa
     9901 gacgacgacg cctttgtcta cgagtttgcg cacggcccta cacacggggt cgtacttgta
     9961 cgagtcaacg gcgggcgctc ccagactcat gttgacgaca cggatgttgt aggccgtgcg
    10021 gttcgccaac agccagtcga gcgcgcccaa aagccccgag accctacccg tgccgcgcga
    10081 gtcgagcacg cgcaggttga ctagtctggc gttgggcgcg acgcccgtat acgcgccgtt
    10141 cgagatgcgc ccgttgccgg cgacgagcgc ggcgacgtgc gtgccgtggc cgtaggggtc
    10201 gtccgtgcgg ccctcgccgg tgaagtcgcg gccgaagacg acgcgggtgg cgttgccgcg
    10261 gtcgaggaaa gatttgtgtg cggcgtcgat gcccgagtcg atgacggcga tcccgacgcc
    10321 cgtcccgtcc agcggtacgg ccgcgccggc ggtgcgcgcg gcgtcggcgc cggtcgtcgc
    10381 cgagacgtgc ccgaggactg agacgcctct gtcgggcgag acgtaggcca cgtcgaggct
    10441 ggcggccagt tgcaggacgg aggcgacggg gatgttcacg gcgcgcgcgt tgaggttcct
    10501 gtagtcagcc ttcaccagcc cgcccaggac acccagcagg aggtcgagcg tcaggcccgc
    10561 ggggccgttc gtttgaacga tcacatcgac gcgctcgccg acgcgcccgc cgagcaccag
    10621 actcagcagg tcgggcgaca ccttgatgat ctgcgtcacg ctctggacgg tctgcgtcag
    10681 ccccccaagg agcgactgcg ccccggccct gtcgccggac acgatgccag accacggggc
    10741 gacgagaagg aacagcgaga cgaaccacgc cgccaatgac ctgcgcttgc tgtctctcac
    10801 tgaacctcct cgcgaaaaca ttagccgggc gacacacgcc gcggacacag gggacgcagt
    10861 tgcccacaat ggcaatcgac gtaccgccgc cgccggcccc atcagcgcga ttttcggccc
    10921 ggaaatgacg ctcgacggtg ggcgcgtcga gcggggcgtt catttcgcgc ttcgtccgcg
    10981 gtcagtatga gctgaccgtc cgtgtcagga tgaacgagtg tgagcgcccc ctgaagagcc
    11041 gcgcgcgccg cgggtttggc aacctgcgaa gacggggtca cagtccgtcg ctctctgcca
    11101 acacattact ggccgtcacg gcgaaatccc tgaaaagcgg cacgcacgcg gtatcggcac
    11161 gcggcttgtc atcaaatcag cgagcgggcg aggggcgggg ccacgggccg agcgccgggt
    11221 gtgcccaatc ccgtcattct ttgctaacat gcccgcgctg ttcgacgaac cccgaggcgg
    11281 gagaggattc cgatggacgc caaagatgac aaagacccgg agcgggcgcg catcgtcgtg
    11341 gtcggcggag gctacggcgg gctgttcacg gccatgcaac tggagcgcgc aggcgcagtg
    11401 acgctcgtca ccaccgagcc gaacttcctg cacacgccgc tgctctacga gtacatgtcg
    11461 ggcgaggtcg aggcgtggca catcgcgccc gcctacgaag agcttctggg cgagcgcatc
    11521 cgcttcgtgc agggcgcggt gacgaatgtt gacttcgagg cccgcgaggt cgaggtggcg
    11581 ggccgcgtcc gcacgctctc ttacgactac ctcgtgctcg ccgtcggcgg cgtaactaac
    11641 ttctggggcg tcgagggcgc tgacaaacac gcgatgccct tccgcaagat tcgccacgcc
    11701 gacgacctgc gcgcgcgcat gatcgaggcg ctcgactcca tccagcccga cgccccgccg
    11761 caggacgcac gccgcgccgc caccttcgtc gtcgtcggcg cgggcgcttc cggcgtcgaa
    11821 ctctcgacga agatgtccga cctgctctac gacgccttca gccgaagagg gttgaagggc
    11881 gagccgcacg tcgttgtcgt cgagatggga cgcgaggcgg tgccggggat ggacgcggag
    11941 attcgccgcg tcgtcgagcg ggcgctctac gagcggcgca tcgaaatcca cacggagacg
    12001 cgcgtcgtga aggtgacgcc cgacggggcc gtcatcgagc acgccggcga gcggcaggag
    12061 atcaaggcgt cgggcgtggt gtggacggcg ggcgtgcgga cgaacccgct cgtcgaaaag
    12121 ttggacgttg agcgcgaccg gcgcggcctc atcgtggtcg agccgtcgat gcaggtgaag
    12181 ggccgccccg gcgtcttcgc cctcggcgac atcgcggctt acacggacgt gccgccgagc
    12241 ctcttcggca cggcgcagct cgcctttcag gagtctgacc tcgtcgccgc caatgtgcag
    12301 gcgctcatcg acggcggcgt cccctcgcgc ggccacttca aggagctggg cgaggccgtc
    12361 agcctcggca ccgccgacgc ggccgtcctc gcctccggtc aggtcgtcac cggccaactc
    12421 gcccgacagg cccgcttcgc cctctacacg acgcgcctgc ccacatggca gcaccgcctc
    12481 aaagtcggcg ccgcatggtt cttcggcggc aagacgccgc gcccgctcgg gctgtgaagc
    12541 cggccaaagt gtgtctaagc cgcatggtgc ggtatcacac gccgcaaggt ggtgttatgc
    12601 cgcaccatgc ggcctaatca atagttagat gcttctttaa tcgccagcct tgaggatgag
    12661 atgaacaagt taattgttgt ttcactgatt gcgacggtga tagtcgcaaa ttttgttgga
    12721 agaaaactga gtggtgctgc gtctaattag cggtaaattg tttggagaag actatgaatg
    12781 aacaaacaga agaccggcgc gattttttga aacgtgcttc aatggcggta atcgcacttc
    12841 cttttttaat cagttgcaaa accgatacgc tggctcaaaa aactgaaaat gaattgagcc
    12901 tgataaagaa aaacgcgatg ccatccggca cggaagcgag gggagcgata gatgttcccg
    12961 acaatgtttc ttggaaaacc tctttgtcga aaaagtctga caaagacgaa ccgatgatta
    13021 tctcggggac gatctttcag cccgacggca gaacgcccgc gccgaacatc ttaatttatt
    13081 tttatcacac tgacagcgac ggctattacg gcagacgcgg cgaagttcga gacggacatt
    13141 ttcgcggctg gatgctaacg ggtgcgaacg gcaaatatga atttagctcg atcaagcccg
    13201 cgccttatcc gaatcgaaca gaggcggcgc acgttcatat gacgctgacc ggaagaaaat
    13261 tcaaagaaga ttccattaac gcgattttgt ttgaaggaga taaatttatc acgacggaag
    13321 aacgaaattc ggtgggaaaa tggggcgaac ctaatcccat tctgaaactc gaaaagggtg
    13381 cggacggcat tttgcgcggc acgcgagatt tcaagttgtg gaaagtttaa tcggattgaa
    13441 tgaaaacaaa gaagacgctt cacatttctg cggcgttgtg cgaagcaaac cgcgtggtga
    13501 aaataacggc gcatctaaca agtcgcggta ggggacggcg ggatagcggg ctgctcatgt
    13561 tgcgtccttc ggcgtatgtt gtgtggtctc gcccgccgcc tctcagctcc agcgttagac
    13621 cgctaaagga atctttccat cggagatgcc agaatgttga agcgagttat ctacgtgcta
    13681 gtcctgagtt gccttgtgat tttcaacacc ctgagtgttg ggtcgggaca gtcaacaacg
    13741 actgacgggc accgcattgt ccccattagg cagatgtctc aggacgttga aattctttat
    13801 ggcgaccctg aaaaggtggg cgagcctttc gtgatgcgca tccgggagct gcccggcggc
    13861 gtcatccctc cccacaagca tccggtcgat gagcatatta ccgtggtgca ggggacatta
    13921 tatttcggcg tgggggataa attcgaccgc gcggctctga aggaattgaa ggccggtagt
    13981 tacgccttca ttcctaaagg gagcacgatg ttcggctaca cgcccgaggc ggcgattgtt
    14041 caggttcacg gcgtcggccc gttccacatt cattggcgcg ccgggactaa ctggcgtgac
    14101 agcctgaaga cgttggacag cccggacgcc gggacggtat tcaagttcag gaggggcgaa
    14161 cgggtgacta ccaaacgcgg gagagggcgc atacgtcagg gttatgattc gggggaaata
    14221 attgggtacg agatcgacgg cgaagacggg aggttgttca tggcagaaga aggggagatt
    14281 aagcgtctcg gagacaagaa atgaattgcg ccggatgcgg cccgcgcggt ctgacaactc
    14341 attcaacccg actctcaatt agcttgtctc ttaaactcag tgttgattgc tcgccggttg
    14401 attcggaggt tagacctcta cgctgtcgca gaaatgtatg agtgaaaagt tagcgctctc
    14461 aaacattctg ggcacagttg ttcgtctccc gacttttagg atttcgcgcg aagactttct
    14521 tcgattcgat aacaagcacc ttgctttcgg cgtagcctgt acctggatag ttggtatcgg
    14581 aagaagtgag atttacccga atggaggggg cacttttttt tgcccgctgc cccttcatca
    14641 gccgccgcct acttcttgat gaattcgacc cgccggttga gggcgcggcc cacgggcgtg
    14701 tcgttgcccg acacgggctt tgacgccccg tagccgaccg tgctcagacg cgccgcatca
    14761 atcccacccg tctgcaagta gcttttcacc gcggcggcgc gacgctgcga caactcctga
    14821 ttgtattgcg cggtcgccgt cgagtcggtg tgtccctcaa tcgtcaaggc ccagtcggtt
    14881 ctcttcttca gcacgcccac gattctatcg agcggcggct tcgactcgcc tcggatcacg
    14941 tccgagccgg ggtcgaaagt gatgctgtag atacgcaccc gcccgaactc ttccaagtcc
    15001 tgctcgaact ggtcgtcggc ttcgggcagg gccgactcgg tcttcggcag cggcgtcatc
    15061 ttcacgcgga gcaccgtggt ctcgccgcgc cggccctcga ccgtggttcg ccaatcgaca
    15121 tacccctcgt gcgccacgcg cacctcgcgc gcttcgcccg gcctcaagcc gtgcgtcagg
    15181 atcccgcttt cctgctggga gacgcccatc ggaatgtcgt caacgaatac cttgctgccg
    15241 tggggcgcgt tcaaaacctt gagcgtgaag ccggaactgg gccacaggag ataggccacg
    15301 aatccgcccg ccagcagcag cgagaacgcc agcaaccccc agcccgcgac ccgcgcccag
    15361 acgggggcgc tgcgctgctg cgcttcggcc ggttgcgccg gctgcggagg aggctgttga
    15421 tagtcggcgg tcggatactg cgccgtgtat ggcggcgcgt gttgttgccc cgcggtctgc
    15481 gaggacactc tggcgtaggc ggcctcgtcg tcctcatccg cgcccagagg gccgtgaatg
    15541 ttcaccatcg tcagatcata cttgttgcct gagccggtcg aaggcggcgg tggcggctgg
    15601 cgtctaccgc cttcaccctg agtagggatg ttgactgacg tgcgatcaaa atcgccccct
    15661 gattggtaag gggtgcgcgc tggaggccgg gggttttgtt gaggcggata gatgttcggc
    15721 gtcgtgaggt cgtaattagg cttccggtcg tcgtcgtctc tgtcactcat aatcgatttc
    15781 cattccggtc acgccgcgcg gtcgtggtga cctctcagct cttcggtcgc cgcctcgcac
    15841 gagtcgagcg ccggcggctc agactcaccc tccggggggt cgccctcacc ggcgtagaag
    15901 aggcccgcgc ggatggtctc gaagtagtcg ccgatgcgtt cgagaatctc cgagacggag
    15961 tgcgagtggt agatgacctg acggaagcgc gcgccgccgg gcaggccgcg cgtgaactgc
    16021 cccgccaact gcttcatgcg gccgaccgcc gccatctcgg gcatgtcagc gcgcaacagc
    16081 tcgaagtatt cgaggagcac ggcgcgcttg tcttcgagcg tcggctcgaa gggttcgcgc
    16141 ccggcggcgg cgtcttcgat ctgccggaaa atccacgggt tcgccatcgc gccgcggccg
    16201 atgagcaggc cgtcgcaatt ggtctcgcgc cagcgggcga aggcgtcttc cacggtgagg
    16261 atgtcgccgc tgccagagac ggggacgctc accacctcct tgatctgccg caccaaatcc
    16321 cagttggccc gccccttgta gccctgctcc ttcgtgcgcc cgtgcagcgc gatgtgctcg
    16381 acgccgcacg cctcggcgag gcgcgccgtc tccagcgcgt tgatgttgta atcgtagtag
    16441 ccggcgcgaa tcttgatggt gagcgggatc gagatggcct tcttgatgtc tttgagaatc
    16501 gtttcgagac gcggcaggtc gcgcagcaga ccggagccgc cgccgtgctt gacgaccttc
    16561 ggcgcggggc agccgcagtt gatgtcgagg atgtcggcgc cgacctcttc ggccagctcg
    16621 gcggccattc tcatccgctc gggccgcccg ccgaagattt gcacggcgaa ggggcgctcg
    16681 tagtcgaaga aacgcatctg ccgcttcgac ttcgggttgt tgcgcgtcag gccctcgacc
    16741 gagatgaact ccgagaccgt caggccgatg ccgccgcgcc gcttgagcag cgcgcgaaac
    16801 ggcacgtcgg tgacgcccgc catcggtgag agcaccagcg gcgggttgat ctcgacatcg
    16861 cgaatcttga agggcttcat aaagcaaccg ggtaaggggt aaaggggaaa gggtaagggt
    16921 ggttcttcgc ctttccccct tcccctttac cccttacccg aatttatttc cagagttcga
    16981 cgcccgtggc gaggaagctg acctcgttgg cggtgccgtc ggggttggtc ttgatcttgg
    17041 cgccgtcgtc tttgacgagg tgttcgacct cttccttcgt cagcaccttg acgctgaatt
    17101 cgccctcggc gcggaaccgc atcttgtcgg cgtccttcgg cacgctgaac ttgtggtcga
    17161 aggtgacgcg gacgctctcg gcggcgcccg cctccggcgc gatctgcatc cagcagcctt
    17221 cggacttgca gacgcgctcg acgacgcctt cgacgaccac gcgcttgccc gcgtacttct
    17281 gcggctcctt caaaacgtgg gcgaagggga cggtcggcga gtcgccgatc ttctcgccgc
    17341 gtctgatgtg ctcgcggtcg tcggcggcct tctcggcggc ggcggtcgtc tgcttgtctt
    17401 gtttgccgcc cttatcgtct gtggagtgct gagtgtgctg ggccgtgacg gcggtagccg
    17461 aggcgaggcc cgcggcgaga aaaagtgtgg cgagtgcagt tttcatggca gaattgaagt
    17521 gcctttcgtt gaattcggaa acatcaatag attgtagaac atcatccgag gctttcgcgc
    17581 atcggaagta tagcaatgcg aggccgactc atccaaagca tcgggatagc gtggacgctc
    17641 ctcttcgggg cggccgtcgt ctggctctac acggcgcaac cccagagcgt ggccgacgtg
    17701 gccacgaaag cgagcgtcac ggccggcacc tacgaggttg accgcgcgcg cgctcaggcg
    17761 gcgcaggcgc tgtttgcgcg ggagcaatac cgggcggcgc gcgacgagtg ggtgcgagcc
    17821 gacccggagc ggagcgacgc gcgcacgcag ttctacgtgg cttacgcctt ctaccgcgag
    17881 ggctggggcc gcgtggggag cgacgacgcg ctcttccggc agggtctcga agccgtcaac
    17941 ctcgccatca gcctctcgcc cggccagacg ctccgcgtgg acgaccccga gctgaaaata
    18001 cagacggcgg ccgaactcaa ggccgagatc gaatcggggc tggagctgac ggccgccgac
    18061 ttcaacccgc tcaaagtttt tcgcgagcgc aagtgattgc cgggagaagt cgtcgatgac
    18121 ggacaaccgc tcaaggaatc gcgcgctcgt cgagtttctg ctgctcccaa ccatcttcct
    18181 gacggtggcg ctgctggggg gcttgcgcgt gagcgccggg acgggcgctt tcgtcttcgt
    18241 cgtcccgccg ctcgtcacgc tcatctgcgc gctgctgctg ctggcgctcg ccgcgcgcgg
    18301 tcgtctcgtc aggctggagc gctggctgag cggcgataac cgcccgctca cgaatgtggc
    18361 gcacgcgctc acgctcgccg cgctcttctt cgcctccgca caggcgttca actcagtgct
    18421 ccccgaggcg gggctgctac gttggatgtt ctcgttcttc tttttgtgga cgctctggaa
    18481 caaccagttc gcgctgttcg acgcgcggcg cttgcttcgc agcctcgccg tcctcttcgg
    18541 caccgccttc ctcctcaaac accttctgct cgccggactc ttcgcgcccg gcgggagtct
    18601 cgcccggcgt ctcgtcggcg ggctgctcga aggcgtcacg ctcggcgcca tcgacgcgcc
    18661 ggcctacgcg cccgccaccg gctacatctc cttcttcaca ctcctgctct acgtcgccgg
    18721 cctcgcgctg ctgccgagcg cgcccacagt ggacgacgcg cccgagggcg cggacgctct
    18781 cctcgcgcag gccgccgacg accgcgcgct ccccgccggc ggcgaggccg tgtttgagac
    18841 ccccgcgatt gaagaacgtc gcgcgcgcgt catcgacatg gacgagtgag cgccgacgag
    18901 tgggatttgt cgcgcgcgtc ggccgccatg acgtgcgctc ggcgcatgcg tcgcgcgtcg
    18961 gaggtggcag gttaaagcga gcgcgctcta ctcggttctt gtctccaaca tttagcggct
    19021 cgtatgctgc tataatttcg gtgaccgcgc tggagcggcg gaaaacgatg atggaacaac
    19081 aggcaaacgg catcgaagac cccggcgcaa gtctggcgcg ggcggcctcg cgcggcgaac
    19141 tcgcgcaggt gcgcctcctc ctcgacgcgg gcgccgaccc gcgggcgaaa gaccggcacg
    19201 gcagcacggc gctgttctac gcggcggggg ccggtcacta cgagatcgcc cgcctcctgc
    19261 tcgaaggcgg agccgacccc gaggcgtcca acgtcgtcgg catgacgccg cacgcggtcg
    19321 ccacgtcgag gggacacgcg cagctcgcgg cgctcatcga gagccacaag accggcggcc
    19381 ggagtggtgg cgtcgaaggg gcgctcgacc gcgggcgtcc cgccctgttg cgcgccgcac
    19441 gcgaaggcga cgcggccggc gtccgcaggc ttttggaggc cggagccgat gcggacacgc
    19501 gcgacgagag cgcatggacg ccgctgatgc tggcggcggt cggcggccac acggaggccg
    19561 tcgggatgct cctcgccggc ggcgctcagc caaacgcccg caacgacaag ggctggacgc
    19621 cgctcatgct cgccgtctcg ctcggcgacg ccggtctggc ccgcgccctc ctcgacgcgg
    19681 gcgccgaccc caacgtcaga gacaacgagg ggctgacgcc gctcatgcag gccgtcggcg
    19741 aaaacaacgt cgagtgcgcg cgtctcctgc tggagcgcgg cgccgacgcc ggcctcacta
    19801 atcactctgg cgagacggcg cagtcaatcg ccgcccggca cgctttcccg gaagtcctcc
    19861 aattgttcgg cgagacggag acgcacaacg ccgacgccgc gacgcccgcg ttcgatctga
    19921 ccagccctga acaactcttt agaaccgagt gggtcggcga gccggatacg gagccgagcg
    19981 cgcgtgatgc ggagccgggc ggcgccgcag tcccggcgga ggtcgaggcc acttccgaga
    20041 gccagtcgct cgttcccgcc cagagcgcgc cggagtcggt cgagagactg atcatggccc
    20101 tcgaagccat gcgccagcag agaccggccg cgccgctcgc ggacatcgcg cacaagctga
    20161 tgctgacgct tcaggaggcg gccgccctca ccggactttc gcagaggcgt ctgcgcgagg
    20221 ccattcgcgc cggacaactg aaggcgcaga tcatcggccg cggctggcgc atcaagcgca
    20281 ccgacctcga agactacgtg cgcgacctct gacattaaga gtctgtttca aaacctcaac
    20341 gcgggtgata actgtagggg cagggcttgt ccgcggccct tgctgtgaag caattaacat
    20401 ggatggacag gataatcagg ataagagatt tgaaatttga aatctcaaat ctgaaatcag
    20461 cgctttcttc atcctaccca tcctgcccat ccatgttaat tcttctgtag gcacgggccg
    20521 cggacttggc ctgcccctac agttttgcca cgcgctttga gtgttaagta tgaggcgcaa
    20581 cagaaaaggg agggcc
//
LOCUS       DASVES010000376         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_362302, whole genome shotgun sequence.
ACCESSION   DASVES010000376 DASVES010000000
VERSION     DASVES010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362302"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2135
                     /locus_tag="VF240_15455"
     CDS             <1..2135
                     /locus_tag="VF240_15455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7176661.1"
                     /translation="QTPQTPATAQDRDEVIRISTEIVQTDVMVFDRSGKFIEGLRPED
                     FDLRVDGRPQRIEFFERIQAGSVDEDAQLRAARGGGVGTVRAASPKDAGKSLPLDRGR
                     VIFFFVDDMHLSAGSAGRLHKMLMRFVDEEMGQNDQAAFASTTGQIGFLQQLTGDKVV
                     LRAAVNRIRHRPYNVKDMETPAMTEAQALAIDQNNPSVIDWFVRQITRENPMISPELA
                     RNMVESRSRALLQQASYITGQTLRTLENLVRGTAQLSARKIVFFVSDGFHIDRRNSDT
                     QHHLQRIADAASRAGAVIYSMDARGLSNDFSDASVKGQFDPEGQLVSTQFDTGASLQE
                     PLRTLAAETGGRALLNTNALGSAVTRSLEETSRYYLLAWRPEDTRARDSKQRRLEVSV
                     RGRPELNVMMRRGYFLTQPAAPSSERAERKKQRAEPEPKTPPADAELFEALRATYPRD
                     SLPTSLALAYVNAGKDTMVLTAALEIDGDQLTYKDEGGTRGAIADLAGAVYDDRGKVV
                     TTLRQELGVLPPLAEVASRQRVVQSYQFRLAPGLYQVRFAARDRGSKRLGSAMQWVEI
                     PDPKKRSFSLSSIFVGERVPSEAPPAAADEGPDAALMRGVTMTADRRLARTSWLRFVT
                     FIYNAANSPVPADVVLQVQVFRDDQPVLTTPLRKIETRDVPDIARIPYAAEIPLSTFP
                     VGHYVLQVTAIDRAAKASASQRINFSVE"
     gene            complement(2237..3832)
                     /gene="serA"
                     /locus_tag="VF240_15460"
     CDS             complement(2237..3832)
                     /gene="serA"
                     /locus_tag="VF240_15460"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX7176662.1"
                     /translation="MTEIKILVADDVSESGLEPLRAAGFAVEKRTGLKGAELSEAVRE
                     CDGLVVRSETKVTAELMESAGRLRAVGRAGVGVDNIDVPAATQRGIIVMNAPDGNTMT
                     TAEHTVALLLALARRVPQGHASLRGGLWERKKFVGVELRGKTLGVVGLGRIGRVVASR
                     ARAFDMKVVAFDPFVAPGQAQDSDVELATLEEVCARADFITVHTPLTPETRGIIGERE
                     FARMKPGVRVINCARGGLVDERALYAAIKDGRVAGAALDVFEQEPPPADHPLLALDEV
                     VATPHLGASTREAQEGVALTVAEQMRDYFLTGALRGAVNVPAIGAEALGALQPYLVLA
                     ERLGRFQAQLIDDPVREVALEYAGEMADTDAAPVTRAFLAGLLRHMSARVNAVNAFLI
                     AGERGIEVTTSHKRGTGGEMRPPVRTRVVTTTGEHTVAGALFGPRSEGRITEIDGFVV
                     EAIPSGHMVVTRSSDVPGVIGRVGTMLGERGVNISRFHLGRRERGGEAIAVIETDGAV
                     GDDTLSELRSFEQVLSARRIELS"
BASE COUNT          654 a         1453 c         1217 g          576 t
ORIGIN      
        1 agcaaacgcc gcagacgccc gccaccgccc aggaccggga cgaagttatc cgcatcagca
       61 ccgagatcgt ccagacggac gtgatggtct tcgacaggtc gggcaagttc atcgagggcc
      121 tgcgccccga ggacttcgac ttgcgcgtgg acggacgacc gcagcgcatc gagttcttcg
      181 agcgcatcca ggccgggagc gtggacgagg acgcgcaact gcgcgccgcc cgcggcggcg
      241 gcgttggcac agtgcgcgcc gcatcgccga aggacgccgg taagtctctg ccgctcgacc
      301 gcggccgcgt catcttcttc ttcgttgacg acatgcacct gtcggccggc agcgccgggc
      361 gtttgcacaa gatgctcatg cgcttcgtcg atgaggagat ggggcagaac gatcaggccg
      421 ccttcgcctc gaccaccggg cagatcggct tcctccagca gctcacgggc gacaaggtgg
      481 tgctgcgcgc cgccgtcaac cgcatccgcc accgccccta caacgtgaag gacatggaga
      541 ctccggcgat gaccgaggcg caggcgctcg ccatcgacca gaacaacccg agcgtcatcg
      601 actggttcgt ccgccagatc acgcgcgaga accccatgat ttcccccgag cttgcccgca
      661 acatggtgga gtcgcgctcc cgcgcgctcc tccagcaagc ctcgtacatc acgggacaga
      721 cgctccgaac gctcgaaaac ctcgtgcgcg ggacggctca actgagcgcc cgcaagatcg
      781 tcttcttcgt ttcggacggc ttccacatcg accgccgcaa ctccgacacg cagcaccacc
      841 tgcaacgcat cgccgacgcc gcctcgcgcg ccggcgccgt catctactcg atggacgcgc
      901 gcggcctctc caacgacttc tcggatgcct cggtgaaggg acagttcgac cccgaaggtc
      961 agctcgtcag cacccagttc gataccggcg cttccctaca ggagccgctg cgcacgctcg
     1021 ccgcggagac gggcgggcgc gccctcctca acaccaacgc gctcggctcc gccgtcaccc
     1081 gttcactcga agagacctcg cgctactacc tcctcgcctg gcgtcccgaa gacacgcggg
     1141 cgcgcgactc gaagcagcgt cgtctggagg taagcgtccg cggccggccc gagttgaatg
     1201 tgatgatgcg ccgcggctac ttcctgacac agcccgccgc cccgtcgagc gagagagcgg
     1261 agcggaagaa gcagcgcgcc gagccggagc cgaagacgcc gccggccgac gccgagctgt
     1321 tcgaggcgct gcgcgccacc tacccgcgcg actccctgcc gacctcgctc gcgctcgcct
     1381 acgtcaacgc cggcaaggac acgatggtgc tcacggcggc cctcgaaatc gatggcgacc
     1441 agctcaccta caaagacgag ggcgggacgc ggggcgccat cgccgatctg gccggcgcgg
     1501 tgtacgacga ccgcggcaag gtggtgacga cgctgcggca ggagttgggg gtactgcccc
     1561 ccttagccga agtcgcctcg cgccagcgcg tcgtccagag ctaccagttc cgcctcgcgc
     1621 cgggcctcta tcaggtgcgc ttcgcagcgc gcgaccgcgg gagcaaacgc ctcggcagcg
     1681 ctatgcagtg ggtcgaaatc cccgatccga agaagaggtc gttctccctg agcagcatct
     1741 tcgtcggcga gcgcgtgccg tccgaagcgc cgccggcagc cgccgacgag gggccggacg
     1801 cggcgctgat gcgcggcgtc acgatgaccg ccgaccggcg gctggcgcgc acctcctggc
     1861 tgcgcttcgt gaccttcatc tacaacgccg ccaacagccc cgtgcccgcc gatgtcgtgc
     1921 ttcaggtgca ggtcttccgc gacgaccaac ccgtgctcac caccccgctc cgcaagatcg
     1981 aaacccgcga cgtgcccgac atcgcgcgca tcccctacgc cgccgaaatc ccgctcagca
     2041 ccttccccgt cggccactac gtccttcaag tcaccgccat cgaccgcgcc gccaaagcca
     2101 gcgcctcgca gcgcatcaac ttttcggtgg aataaaaaaa ggagtcagga gtcagaagtc
     2161 agaagtcaga ataaagaagg cgaaccccgc ttttattctg acttctgact cctgactcct
     2221 gactcctttt ccttgcctaa ctcagctcga tgcgccgggc cgagagcacc tgctcgaagg
     2281 agcgaagctc ggagagcgtg tcgtcgccca cggcgccgtc tgtttcgatg acggcgatgg
     2341 cctcgccgcc gcgctcgcgc cggccgaggt ggaagcggct gatgttgacg ccgcgctcgc
     2401 cgagcatggt gcccacgcgc ccgatgacgc cgggcacgtc gctgctgcgt gtgacgacca
     2461 tgtgacccga cgggatcgcc tcgacgacga agccgtcgat ctcggtgatg cgtccctcgg
     2521 agcgcggccc gaagagcgcg cccgccaccg tgtgctcgcc ggtggtcgtc acgacgcgcg
     2581 tcctcacggg cggccgcatc tcgccgcccg tcccgcgctt gtgcgaagtc gtcacctcga
     2641 tgccgcgctc gccggcgatg aggaaggcgt tgacggcgtt gacgcgcgcg ctcatgtggc
     2701 gcagcaggcc ggcgaggaag gcgcgcgtca cgggcgcggc gtccgtgtcg gccatctcgc
     2761 cggcgtactc cagagcgacc tcgcgcacgg ggtcgtcgat caactgcgcc tggaagcggc
     2821 ccaatcgctc ggccagcacg aggtacggct gcaaggctcc aagcgcctcc gcgccgatgg
     2881 cggggacgtt gacggcgccg cgcagcgcgc ccgtcaggaa gtagtcgcgc atctgctcgg
     2941 cgaccgttaa ggccactccc tcctgcgcct cgcgcgtcga agcgccgagg tgcggcgtcg
     3001 ccacaacttc atcgagcgcg agcaacgggt ggtcggccgg cggcggctcc tgctcgaaca
     3061 cgtcgagcgc cgcgcccgcc acgcgtccat ccttgatggc cgcgtagaga gcgcgctcgt
     3121 ccacgaggcc gccgcgcgcg cagttgatga cgcgcacgcc cggtttcatg cgcgcgaact
     3181 cccgctcgcc gatgatgccg cgcgtctcgg gcgtcagggg cgtgtggacg gtgatgaagt
     3241 cggcgcgcgc gcaaacctct tcgagcgtcg ccagctccac gtccgagtcc tgcgcctgac
     3301 cgggcgcgac gaacgggtcg aaggccacga ccttcatgtc gaaggcgcgc gcccggctcg
     3361 ccacgacgcg cccgatgcgc ccgaggccga caacgccgag cgtcttcccg cgcagctcga
     3421 cgccgacaaa cttcttgcgc tcccacagtc cgccgcgcaa actcgcgtgg ccctgcggca
     3481 cgcgacgcgc gagcgcgagc aagagcgcca ccgtgtgctc ggccgtcgtc atcgtgttgc
     3541 cgtccggcgc gttcatcacg atgatgccgc gctgcgtcgc cgccggcacg tcgatgttat
     3601 caacgccgac tcccgcccgc ccgaccgccc gcagcctccc ggccgactcc atcaactccg
     3661 ccgtcacctt cgtctcgctg cgcacgacca gaccgtcgca ctcgcgcacg gcctccgaca
     3721 gttccgcgcc cttcagcccc gtcctcttct cgacggcaaa ccccgccgcc cgcaacggct
     3781 ccagcccgct ctcgctcaca tcgtcggcaa ccagaatttt aatttcagtc ataaaaacag
     3841 aagtcaggag tcaggagtca ggagccagaa tgaagtccgc gttcgccttc ttcatactga
//
LOCUS       DASVES010000377         7470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_645064, whole genome shotgun sequence.
ACCESSION   DASVES010000377 DASVES010000000
VERSION     DASVES010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645064"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..3560
                     /locus_tag="VF240_15465"
     CDS             105..3560
                     /locus_tag="VF240_15465"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX7176663.1"
                     /translation="MRSSNNCVNRSLSRALFVLCALSLLIASVALTRPDAPSPARGGA
                     SVETPLTTKAVSETYGQIPLSFEANRGQTDESVNFLARGAGYTLFLKPTEAVFTLTRR
                     GGAPGAVSAEQSMPDASTPTARNVGAPDTAGTERPAVLRMKLVGADARASVEGAEELA
                     GRVNYFNGNDPAKWRTDVPTFGRVRYPEVYPGIDVVYYGNQRQLEYDFVVAPGVDARS
                     IALEFDGADKLEVDAAGDLLLTLGEEVIRQPKPLVYQEVAGARREVAGGYALEEGGRV
                     RFALGEYDARLPLVIDPVLVYSTYLGGSNTDLARAIAVDSSGRAYVTGYTNSTNFPTA
                     NAFQASLNGTAGAFGNRDVFVTKLNAEGTAFVYSTYLGGSGGEEARGIAVDSLGSAYV
                     AGFTSSTNFPIANAIQATKGATNEDAFVTKLNADGTALVYSTYLGGGESSEFGEAIAV
                     DSSGAAYVTGSTFSDDFPTLNPIQATFGGGSTDAFVTKINADGTALVYSTYLGGSGSP
                     GSTQPETGFGIAADSAGNAYVTGETNSANFPVANAVQGTFGGGISDAFVAKISATGNA
                     FVYSTFLGGGAQDAGEGVKADASGNTYVAGFTVSTNFPVANALQGANAGGQDAFVTKL
                     NANGSTLVYSTYFGGTGGEIAFGIAIDASGSVYLGGSTGSTNTLPTANAIQCTRNGGQ
                     DTFIAKFNATASALVYSTYLGGSGSDDGQGIALDPSNNAYLTGSTTSTDFPTVNPIQS
                     ANAGGSPFGDAFVTKVSDNTTGPASQLQFTQTAPSVQEDVTSLTLTVQRIGDTAGPVT
                     VDYATADGTASERSDYTTALGTLSFAAGESAKNVVLLINEDSKVEGNETFTVTLSNPT
                     GGGTLSCLTATATVQITDDTSEPATNVIDDASIFVGQHYHDFLNRQSDPAGQAFWTEQ
                     LTACGADAACIDERRQNVSAAFFLSIEFQRTGYEVIRIYKATFTDSPGRPRGLPRYRE
                     FLRDTQEVARGIVVGHGPWEQQLDDQPLAFARRWVQTTEFVAQFPLGTPAGQYVDKLF
                     LNSEVTPTTAERDAAIAAYGAGDTEGRAQALLSVTRSNSVYNRQYNPAFVLMQYIGYL
                     RRNPNDAPDASYAGYDFWLTKLNQFSVAGEDVVNENVALGRVRRAEMVRAFIISGEYR
                     GRFGP"
     gene            complement(3757..4548)
                     /locus_tag="VF240_15470"
     CDS             complement(3757..4548)
                     /locus_tag="VF240_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176664.1"
                     /translation="MSRRLTSRTRVKFLTHLLRLPTHSALAGASFGVAFAIASLLLAP
                     QTLAKRVRAPGGARAAVVVDERLAALRDAPGLSANLVQRLGRGRAVSLRGPKVARDGV
                     TFYRVGVTSRTSGWVQGEALASSARAGDDERLLRLISGSEGYDRLERAVIFIETFPRS
                     PLRPAALLLLGETADDAARTLSREAARRLDEREMAAGGAPPHSYFLNFSGLDRYRRQG
                     IIFTFDAAKKQFQYDGAAWRELVRRHPRSPEAERARARLGERGMK"
     gene            complement(4620..5180)
                     /gene="ruvC"
                     /locus_tag="VF240_15475"
     CDS             complement(4620..5180)
                     /gene="ruvC"
                     /locus_tag="VF240_15475"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7176665.1"
                     /translation="MIVLGIDPGSEITGWGVIEGTPRRYRMINYGVVKANARERFPAR
                     LLKIHAGVEGVIEKYRPDVCAVEESFFAVNPKTALKLGHVRGVVLLAAEKAGLEIAEY
                     APRLIKQTVVGYGAAEKHQVQAMVRVLLALKELPKPFDAADALATAICHMHHAGLASL
                     KAKTPPARARLEALLATTNTRRRAPR"
     gene            complement(5312..6055)
                     /locus_tag="VF240_15480"
     CDS             complement(5312..6055)
                     /locus_tag="VF240_15480"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7176666.1"
                     /translation="MSGHSKWHSIKHKKGALDAKRGKLFTKLIKEISVAARTGGGDPD
                     ANARLRKAISDAKGANMPNDTIDRAVQRGAGQEGGVIYDEITYEGYGPGGVAVMVDSM
                     TDNRNRTVAEIRHIFGKNGGNLATSGAVNWIFEKKGYIVVGKDAKPEEELFEIVTEAG
                     AEDLRDDEDNFEIITAPADFEQVLDAVKRAGIEPHVAEVDMVPKEYKKLEGAEAKQML
                     KLVEALEDNDDVQKVFANFDISEADMASA"
     gene            complement(6213..6500)
                     /locus_tag="VF240_15485"
     CDS             complement(6213..6500)
                     /locus_tag="VF240_15485"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX7176667.1"
                     /translation="MIRYKESDGRITFDVRVVPRASKTAVAGEHDGALRVRVAAPPVE
                     GAANEELVRFLARELGVAVRSVEIVAGHTSKSKRVRVTGACVELISKLAEE"
     gene            complement(6513..7190)
                     /locus_tag="VF240_15490"
     CDS             complement(6513..7190)
                     /locus_tag="VF240_15490"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX7176668.1"
                     /translation="MFQRFEAVEAPAPTAAAGAITAIGYVFLFVWYVIVAVIVAMIAL
                     MVLRFILNYADLNPFSRPVIFVRRLTDPFVNPVRRALLGFGIKPNGAPLFVILLTILL
                     GYFVLMLAASVLNTAAGIALSALSGRAGGIVALLGYILYGLLSLYSLLIVVRIIFSWG
                     QVSYSNRVMRFLMNATDPILLPLRRIIPPLGMFDISPIVAFLIIWLFQGAIAMTLLRG
                     WPIAFYM"
     gene            complement(7251..>7470)
                     /locus_tag="VF240_15495"
     CDS             complement(7251..>7470)
                     /locus_tag="VF240_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX7176669.1"
                     /translation="PWSDDPELARPWFRRLRALQQTWIGTGVPADALRHLSMGMSHDF
                     EAAIEEGATMVRVGTAIFGARAPRPASE"
BASE COUNT         1321 a         2497 c         2385 g         1267 t
ORIGIN      
        1 gctcaccgaa gcaagaccgc tctttaactc acgcgcaata tctaaagtcc ccgcggcaat
       61 cttgcgggga atatacccga agcgattcaa aaagtgagga tgagatgaga tcgtcaaaca
      121 actgtgtcaa ccgctccctc agccgggcgc tcttcgtgct gtgtgccttg agtctactca
      181 tcgcgtcggt ggcgctgacc cggccggacg ccccatcgcc cgcgcgcggc ggcgcgtccg
      241 tggaaacgcc gctcacgacc aaagccgtga gcgagacata cggccagata cctttgagct
      301 tcgaggccaa ccgggggcag accgacgagt cggtcaactt cctcgcgcgg ggcgcgggct
      361 acacgctgtt cctgaagccg accgaggccg tcttcacttt gacgcgccgg ggcggcgctc
      421 ccggcgccgt gagcgccgag cagtcgatgc ctgacgcctc gacaccgacg gcgcgcaacg
      481 tgggcgctcc cgacaccgcc ggcacggagc gcccggcggt gctccgcatg aagctcgtcg
      541 gcgcggacgc gcgcgcgtcg gtcgaaggcg cggaagagct ggcgggccgg gtcaactatt
      601 tcaacggcaa cgatccggcc aaatggcgca cggacgttcc caccttcggg cgcgtgcgct
      661 acccggaagt ctatccgggc atcgacgtgg tgtattacgg caaccagcgt cagctcgaat
      721 acgacttcgt ggtcgcgccg ggtgtggacg cgcgctccat cgcgcttgag ttcgacggcg
      781 cggacaagtt ggaagttgac gcagcgggcg acctgctgtt aacgctgggc gaggaggtga
      841 ttcgacagcc gaagccgctc gtctatcagg aggtggcggg cgcgcggcgc gaggtcgcgg
      901 gcggctacgc gctggaggag ggcggacgcg tccgcttcgc gctcggcgag tacgacgcgc
      961 ggctcccgct cgtcatcgat ccggtcttag tctattccac ctacctcggc ggcagtaaca
     1021 ccgatctcgc cagagccatc gctgtggact cctccggcag agcttacgtc acgggttaca
     1081 ccaactcgac taatttcccg acggctaacg cctttcaggc gagtcttaat ggtacggccg
     1141 gcgcttttgg caacagggat gtcttcgtca ccaagctcaa cgcggagggc acggccttcg
     1201 tctactcgac ttacctcggc ggcagcggcg gcgaggaggc cagaggcatc gccgtggatt
     1261 ctttgggcag cgcctacgtc gccggcttca cctcgtcaac caacttcccc atcgccaacg
     1321 ccatacaagc cacgaagggc gcgaccaacg aggacgcttt cgtcaccaag ctcaacgccg
     1381 acggcacggc cctcgtctat tcgacctacc tcggcggcgg tgaaagttcg gagtttggcg
     1441 aggccatcgc ggttgattct tccggcgcgg cttacgtcac cgggagcacc ttctcggacg
     1501 acttccccac cttgaacccc attcaagcca cgttcggcgg cggctccacc gacgctttcg
     1561 tgacgaagat caacgcggat ggcacggcgc tcgtctattc gacttatctc ggcggctctg
     1621 gctcccccgg cagcactcaa ccggagaccg gctttgggat cgcggctgat tccgcgggca
     1681 acgcctacgt cactggagaa accaactcgg ccaacttccc cgtggccaac gccgtgcagg
     1741 gcacgttcgg cggcggcatc tccgacgctt tcgtcgccaa gatcagtgcg acgggcaacg
     1801 cgttcgttta ctcgactttc ctcggcggcg gcgcgcagga tgctggcgaa ggcgtcaagg
     1861 ccgacgcctc cggcaacaca tacgtcgccg gattcaccgt gtcaaccaac ttccccgtgg
     1921 ccaacgccct gcaaggcgcc aacgccggcg gtcaggacgc tttcgtcacc aagctcaacg
     1981 ccaacggttc gacgctcgtc tactcaactt acttcggcgg cacgggcggc gagatcgctt
     2041 tcggcatcgc gattgacgcc tccggcagcg tgtatctcgg gggaagcacc ggctcgacaa
     2101 acacccttcc gacggccaac gccattcagt gcacgcgcaa cggcggccag gacaccttca
     2161 tcgcgaagtt caacgcgacg gcctcggcgc ttgtctactc gacctatctc ggcggcagcg
     2221 gctccgacga cggacaaggc atcgcgctcg acccatcgaa caacgcttac ctcaccggct
     2281 caaccacttc gaccgacttc cccacggtca accccatcca aagcgcgaac gccggcggct
     2341 caccgttcgg cgacgccttc gtgacgaagg tcagcgacaa caccacgggg ccggcctcgc
     2401 agctccagtt cacgcagacc gcgccctccg tgcaggagga cgtgacctcc ctcacgctca
     2461 ccgtccagcg catcggcgac accgccggcc ccgtcaccgt tgactacgcg actgccgacg
     2521 gcacggcctc cgagcgcagc gactacacca ccgccctcgg caccctcagc ttcgccgccg
     2581 gcgagtcggc gaagaacgtc gtcctgctca tcaacgaaga ctcgaaggtc gaaggcaacg
     2641 agacgttcac cgtcacactc tcgaatccaa cgggcggcgg cacgctctcg tgcctgacgg
     2701 cgacggccac cgtccagatc acggacgaca cgtcggagcc ggcgaccaac gtcatcgacg
     2761 acgcgtcgat cttcgtcggc cagcactacc acgacttcct caaccgccag agcgaccccg
     2821 ccggtcaggc gttctggaca gagcaactca ccgcctgcgg cgcggacgcc gcctgcatcg
     2881 acgagcgccg gcagaacgtc tcggcggcct tcttcctctc catcgagttc cagcggacgg
     2941 gctacgaagt gatccgcatc tacaaggcca cgttcaccga cagccccggc cgcccgcgcg
     3001 gcctgccgcg ctaccgcgag tttttgcgcg acacgcagga ggtggcgcgc ggcatcgtgg
     3061 tcggtcacgg cccgtgggag cagcagttgg acgatcagcc gctcgccttc gcgcgccgct
     3121 gggtgcagac gacggagttc gtcgcgcagt tccctctggg aacgccggcg gggcagtacg
     3181 ttgacaagct gttcctcaac tcggaggtga cgccgacgac ggccgagcgc gacgcggcca
     3241 tcgcggccta cggcgcgggc gacacggagg ggcgcgccca agcgctgctc tcggtgactc
     3301 gcagcaactc ggtttacaac cggcagtaca acccggcctt cgtgctgatg cagtacatcg
     3361 gctacctgcg ccgcaacccg aacgacgcgc ccgacgccag ctacgcgggc tacgacttct
     3421 ggctcacgaa gctcaatcag ttcagcgtgg ccggcgagga cgtggtcaac gaaaacgtgg
     3481 cgcttggcag ggtgaggcgc gccgagatgg tcagggcctt catcatctcc ggcgagtacc
     3541 gcggccggtt cgggccgtga tattgcagct atcagttgca gtaggtagta agtcggattc
     3601 accatcgccg gattcgcaaa gggcgcgggt tcgcgtagag agattcaacc ctccgcgctt
     3661 cctccgcgcc ctgcggcgcc tctgcggtga atctacttcc ctttcactca actcacaaaa
     3721 tcactctaat cacgcgcgac gaccgtcgcg gggcgctcac ttcatccccc gctccccgag
     3781 gcgtgcgcgc gccctctcgg cctccgggct gcgcgggtgg cggcgcacta actcgcgcca
     3841 cgccgcgccg tcgtattgaa actgcttctt cgcagcgtcg aaggtgaaga tgattccctg
     3901 tcttcggtag cggtcgaggc cgctgaagtt gaggaagtag ctgtgcgggg gcgcgccgcc
     3961 ggcagccatc tcgcgctcgt cgagacggcg cgcggcctcg cgagagagag tccgcgccgc
     4021 gtcgtcggcc gtctcgccga gcaggagcaa cgccgcgggc cggagcggcg agcgcggaaa
     4081 cgtttcgatg aaaatgacgg cgcgctccag acggtcgtag ccctcggagc cgctgatgag
     4141 tcgcaacagg cgctcgtcgt cgccggcgcg cgcgcttgag gccagcgcct cgccctgcac
     4201 ccacccactc gtccggctcg tcacgcccac gcggtagaaa gttacgccgt cgcgcgcgac
     4261 cttcgggccg cgcaacgaca ccgccctccc gcgcccaagt cgctgcacaa ggttcgccga
     4321 aaggccgggc gcgtcgcgta gcgcggccag acgctcgtcc acgacgacgg ccgcgcgcgc
     4381 gcccccgggc gcgcgtactc tcttcgccag agtctgcggc gcgagcagta aagaagcgat
     4441 tgcgaaagca acaccaaagg aagcgcctgc gagggctgag tgcgttggca gacgcaggag
     4501 atgtgtcagg aatttgacac gcgtgcgact tgtgaggcgt cgagacatgg gcttactcct
     4561 tttcgggtcg gtgaggttga aaggaggcgt gccgcgtgcg gccccgtttg cgtgagagtt
     4621 cagcgggggg cgcggcgtcg cgtgttcgtt gtcgctaaca aagcttcgag ccgggcgcgg
     4681 gccggcggcg tcttcgcctt caatgacgcc agccccgcgt ggtgcatgtg gcagatggcc
     4741 gtggcgaggg cgtcggcggc gtcgaagggc ttgggcagct ccttgagcgc gagcagcacg
     4801 cgcaccatcg cctgcacctg atgcttctcg gcggccccgt agccgacgac cgtctgcttg
     4861 atgagccgcg gcgcgtactc ggcgatctcc agcccggcct tttccgccgc cagcagcacg
     4921 acgccgcgca cgtggccgag cttgagcgcc gtcttcggat tgacggcgaa gaacgactcc
     4981 tcgacggcgc acacgtcagg ccggtacttc tcgatgaccc cctcgacgcc cgcgtgaatc
     5041 ttcaacaacc gcgccgggaa ccgctcgcgc gcattcgcct tcaccacgcc gtagttaatc
     5101 atccggtaac gccgcggcgt gccctcgatg acgccccacc cggtgatctc gctgcccgga
     5161 tcgatgccta gaactatcat agagtgataa gtgatgagtg ataagtgatg agaaatagct
     5221 gttagcgatt agcaattagc tgctcggctg aaagctaaca gctaacggct aacagctctt
     5281 atcacttatc actcatcact tatcacggcc gtcaggccga agccatgtcg gcctcgctga
     5341 tgtcgaagtt ggcgaagacc ttctgcacgt cgtcgttgtc ttcgagcgcc tcgacgagct
     5401 tgagcatctg tttggcctcg gcgccttcga gctttttgta ctccttgggc accatgtcca
     5461 cttcggccac gtgcggctcg atgccggcgc gcttgacggc gtcgagcacc tgctcgaagt
     5521 cggcgggcgc ggtgatgatc tcgaagttgt cctcgtcgtc gcgcaggtct tcggccccgg
     5581 cctcggtaac gatctcgaac agctcctcct cgggcttggc gtctttgccg acgacgatgt
     5641 agcccttctt ttcaaatatc cagttgaccg cgccggaggt ggcgaggttg ccgccgttct
     5701 tgccgaagat gtggcgaatc tcggcgaccg tgcggttgcg gttgtcggtc atcgaatcga
     5761 ccatcacggc gacgccgccg gggccgtagc cctcgtaggt gatctcgtcg tagatgacgc
     5821 caccctcctg accggcgccg cgctggacgg cgcggtcgat ggtgtcgttc ggcatgttgg
     5881 cgcccttggc gtcggagatg gccttgcgca gccgcgcgtt ggcgtccggg tcgccgccgc
     5941 ccgtgcgggc cgccaccgag atttccttga tcagcttcgt gaagagcttg ccgcgcttgg
     6001 cgtcgagcgc gcccttcttg tgtttaatgg agtgccactt ggagtgtcct gacatgacgg
     6061 atgagttcct tgaatttact ggtgttcgtt cggcgcggaa gcgggtcgga atatatcacg
     6121 cggtttttcg cgcccgcaac cggggaatgc gaacagcgtt cagcaatcga cgttcagcct
     6181 ccatgcccct gttcagcatc gcttccttgt catcactctt ccgccaattt gctgatgagt
     6241 tcaacacacg cgcccgtgac ccggactctc ttggacttcg acgtgtggcc ggcgacgatc
     6301 tcgacggagc gcaccgcgac gcccagctcg cgcgcgagaa agcgcaccag ctcctcgttg
     6361 gccgcgccct cgacgggcgg cgcggcgacg cgcacgcgca acgcgccgtc gtgctcgccg
     6421 gccacggcgg tcttagaagc gcgcgggacg acgcgcacgt cgaaggtgat gcggccgtca
     6481 gattccttgt agcggatcac gccggaagcc gctcacatgt agaaggctat gggccagccg
     6541 cgcaggagcg tcatggcaat cgcgccctga aagagccaga tgatgaggaa ggcgacgatg
     6601 ggcgagatgt cgaacatgcc gagcgggggg atgattcgcc ggagcggcag caggatgggg
     6661 tcggtcgcgt tcatcaggaa gcgcatcacg cggttcgagt aggacacctg cccccacgag
     6721 aaaatgatgc ggacgacgat gagcaggctg tagagcgaga gcaggccgta gaggatgtag
     6781 ccgagcagcg cgacaatgcc gccggccctc ccgctgagcg cgctcagggc gatgccggcc
     6841 gcggtgttca ggacgctggc ggcgagcatg agcacgaagt agccgagcag gatcgtcagc
     6901 aggatgacga agagcggggc gccgttgggc ttgatgccga agcccagcag tgcgcggcgg
     6961 acggggttga cgaacgggtc ggtcaggcgt cggacgaaga tgacggggcg gctgaagggg
     7021 ttgaggtcgg cgtagttgag gatgaaacga aggaccatca gcgcgatcat ggcgacgatg
     7081 acggcgacga tcacgtacca cacgaagagg aagacgtaac caatcgccgt tatcgccccg
     7141 gccgcggcgg tgggggcggg agcctcgacg gcttcgaatc tctgaaacat caaaccgagc
     7201 gtcttcataa aactttcgcc tcgataaaaa ctttctcaac gtataccgat tcactcggaa
     7261 gccggacgcg gagcgcgcgc cccgaagatg gccgtcccga cgcgcaccat cgtcgcgccc
     7321 tcctcgatgg ccgcctcgaa atcgtggctc atgcccatcg agaggtgccg cagcgcgtcg
     7381 gccggaacgc cggtgccaat ccacgtctgc tgtagggctc gcagccggcg gaaccagggc
     7441 cgggccagct ctggatcatc gctccacggt
//
LOCUS       DASVES010000378         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_118095, whole genome shotgun sequence.
ACCESSION   DASVES010000378 DASVES010000000
VERSION     DASVES010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118095"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VF240_15500"
     CDS             <1..964
                     /locus_tag="VF240_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7176670.1"
                     /translation="RDGHEVTLFNRGQHNPELFPEVEKLRGDRDGGLDALRGRSWDAV
                     IDTCGFVPRVVRASAELLAGAVGLYVFISTISVYPDYSRPVDEDSPVGKLEDESREDY
                     KGEAYGPLKALCERAVEEALPGRTLVVRPGLIAGPHDPTNRFAYWPRRVARGGEVLAP
                     APPERLVQFIDARDLADWTIRMAASGATGVFNATGPDYKLSMGALLEECRRVLGGDAR
                     FTWVDEQVLLENKVEAWSELPLWITGEDPAMRYFMEANCGRAFKAGLTFRPLAETVRD
                     TFEWDESQAGRAPAPDDRPDATLKPERERELLEAWHKTKAAAGA"
     gene            complement(1050..1475)
                     /locus_tag="VF240_15505"
     CDS             complement(1050..1475)
                     /locus_tag="VF240_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7176671.1"
                     /translation="MTIIRNVVCGREPYFVRESASVLEAAEFMASRNIGAVCVLNDGD
                     RLSGIFSERDLLQRVIVAGLDPASLPIRDVMSPPRAVISCDETPHEALERMEQVGSRH
                     LPVMDGERWIGMLSMRDIMRVEISEQGAELKLLHEYIQH"
     gene            complement(1563..2594)
                     /locus_tag="VF240_15510"
     CDS             complement(1563..2594)
                     /locus_tag="VF240_15510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7176672.1"
                     /translation="MRHVENPPNPWLHTSVEWVGEPPESKVEVFEETETRRIITHNNS
                     PDVGFDYSVNCYRGCTHACSYCFSRPTHEYLGYGAGTDFETKIVAKVRAPDLLRAEFL
                     RPSWAGHTLVFSFTSDPYLPLEAHYKLTRRCLEVCLEFRNPVGIVTKSALVRRDAQLL
                     AALARDASASVFFTIPFDDYQTARALEPFAPSPDARFRAMRELSDAGVPVGLGISPVI
                     PGLNDPHIPRLLKRAREAGAREAFMGMLKLPGSVAPYFEQRLRERLPTKADRVMARVR
                     EAREGKLNSSAFGERMRGKGEYWRMVERSFRVHCERLGMNEDGGGADDDGERPNTFRR
                     PTAQVSLFD"
BASE COUNT          480 a          895 c          985 g          479 t
ORIGIN      
        1 gcgtgacggg cacgaggtga cgctcttcaa tcggggccag cacaacccgg agcttttccc
       61 ggaggttgag aaactgcgcg gcgaccgcga cggcggtctc gacgccttgc gcggacggag
      121 ttgggacgcc gtgatcgaca cctgcgggtt tgtgccgcgc gtcgtgcgcg cgtcggccga
      181 gttgctggcc ggggctgttg gtctctacgt cttcatctca accatctccg tctaccccga
      241 ctacagccgg cccgtggacg aagactcgcc cgttggcaaa ctcgaagacg aaagccggga
      301 ggattacaag ggtgaggcat acgggccgct gaaggctctg tgtgagcgcg cggtggagga
      361 ggcgctgccc ggccgcacgc tcgtcgtccg ccccggcctc atcgccgggc cgcacgaccc
      421 gaccaaccgc ttcgcctact ggccgcgccg cgtcgcccgc ggcggcgaag tgctcgcgcc
      481 cgccccgccc gagaggctgg tgcagttcat cgacgcgcgc gacctcgccg actggacgat
      541 tcgcatggcc gcgtcgggcg cgaccggcgt cttcaacgcc acgggaccgg attacaaatt
      601 gtcgatgggc gcgctccttg aagaatgtcg tcgcgtgttg ggcggcgacg cgcgcttcac
      661 atgggttgat gaacaagttc ttctggagaa caaggtggag gcgtggtcgg agttgcccct
      721 gtggatcacc ggcgaagacc cggcgatgcg ctacttcatg gaggcgaact gcgggcgggc
      781 cttcaaggcc ggtctcacct tccgccccct cgccgagacc gtccgcgaca ctttcgagtg
      841 ggacgagagt caagcgggcc gtgcgccggc cccggatgac aggccggacg cgaccctcaa
      901 gccggagcgc gagcgcgaac tgctcgaagc gtggcacaag acgaaagcgg cggcgggcgc
      961 gtaaacagta ccgcgagcgg tagcgagcgg gtgttgcccc gtgggacgcg aacccgcgag
     1021 gcttccgctc gcgatactgt tttgaaaagt cagtgctgga tgtattcgtg gaggagtttc
     1081 agctcggcgc cctgttcgct gatctcgacg cgcatgatgt cgcgcatcga gagcatcccg
     1141 atccagcgct cgccgtccat cacgggcagg tgccggctcc cgacctgctc catccgctcc
     1201 agcgcctcgt gcggcgtctc gtcgcaactg atgacggcgc gcggcgggct catcacgtca
     1261 cggatgggga gcgacgccgg gtcgagtccc gcgacgatga cgcgctggag gaggtcgcgc
     1321 tcggagaaga tgccgctcag gcggtcgccg tcgttgagca cgcagacggc cccgatgttg
     1381 cggctcgcca tgaactcggc agcctccagc acgctcgccg actcgcgcac gaagtaaggc
     1441 tcgcgcccgc agacaacatt tcggatgatc gtcatcgccc ttccactccc ctccggccgt
     1501 aaattccgaa cacgaactcg cgggcgcatt ataagcccga acgggcgtga cttttcaagg
     1561 ttttagtcga agagcgacac ttgcgccgtg ggcctgcgga aagtgttcgg cctttcgccg
     1621 tcgtcatccg ccccgccgcc gtcctcgttc atccccagcc gctcgcagtg aacgcggaag
     1681 ctgcgctcga ccatcctcca gtactcgcct ttcccgcgca tcctctcgcc gaacgcgctc
     1741 gaattcaact tcccctcgcg cgcctcgcgc acacgcgcca tgacccggtc ggccttcgtc
     1801 ggcaggcgct cgcgcaaacg ctgctcgaag taaggcgcga cgctgccggg caacttgagc
     1861 atgcccatga aagcttcgcg cgcgccggcc tcgcgcgcgc gtttcaggag gcgcgggatg
     1921 tgcgggtcgt tgaggccggg gatgacgggg gagatgccta gcccgacggg gacgcccgcg
     1981 tccgagagtt cgcgcatcgc gcggaagcgc gcgtcgggtg agggggcgaa cggctcgagg
     2041 gcgcgcgccg tctggtagtc gtcgaagggg atggtgaaga agacgctggc cgacgcgtcg
     2101 cgcgcaagcg cggcgaggag ctgcgcgtcg cggcggacga gggccgactt ggtgacgatg
     2161 cccacggggt tgcgaaactc aaggcagact tcgagacagc ggcgcgtgag tttgtaatgc
     2221 gcttcgagcg ggagatatgg atcggaggtg aaggagaaga cgagcgtgtg gccggcccac
     2281 gacgggcgca ggaactcggc gcgcagcagg tcgggggcgc gcaccttggc gacgatcttg
     2341 gtctcgaagt cggtgccggc tccgtagccg aggtattcgt gcgtcgggcg gctgaagcag
     2401 tagctgcacg catgtgtgca gccgcgatag cagttgacgg agtagtcgaa gccgacatcg
     2461 gggctgttgt tgtgggtgat gatgcgtcgg gtctcggtct cctcaaagac ctcgaccttc
     2521 gactcgggcg gctcgcccac ccactcaacg ctcgtgtgga gccaggggtt cgggggattc
     2581 tctacgtggc gcattgattt actcccgcgc ggccgggccg cgtgccgaac tggatttgtc
     2641 ggatcgggag cttgcggaag ttattttaca tcattactaa caaaagcaag gatgaaggat
     2701 gaaagcggaa ggatgaataa agattgattt actgattcaa tgagggctgg ccggcgcgca
     2761 cagtaccgcg agcggaagcc tcgcgggtct cgcttgcgcg gggcgggcct cacatcacag
     2821 tgacacccgc tcgctaccg
//
LOCUS       DASVES010000379         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_645933, whole genome shotgun sequence.
ACCESSION   DASVES010000379 DASVES010000000
VERSION     DASVES010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645933"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VF240_15515"
     CDS             <1..1197
                     /locus_tag="VF240_15515"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7176673.1"
                     /translation="GLIKIMNGEIFILGGKRTPMCSYVGALKDVKAIDLGATAARGAL
                     EATGVAAEEIDHTVFGNALQTSGDAIYGARHVALKAGVPAERPALTVNRLCGSGIQSI
                     VSGAHMILLGEAETALVGGMESMSMAPHAIYGARAGFGLGQGKLEDTLMVALLDTYCN
                     TTMAGTAENLARRYEISREEQDKYALRSQQEAARAQGQGVFAEEIVPVEVKTRKGVKT
                     IDTDDHLRPDTTLEILAKLPTAFSKDGFVTAGNASGIVDGAAALVIAGAEYVRRKGAK
                     PAARIVSWAYAGVEPEVMGIGPVPAIRKALEKANLKLEEIDLVEVNEAFAGQYLAVEK
                     ELGLERARTNVNGGAIALGHPLGASGTRLVLTALLELQRRGGRYGLASACIGGGQGIA
                     IVFERI"
     gene            1329..3188
                     /locus_tag="VF240_15520"
     CDS             1329..3188
                     /locus_tag="VF240_15520"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEX7176674.1"
                     /translation="MSAFPRAPEIFLPNRRHLLTPAALAACALFVCLITSAVTARAQE
                     SFAPNIVISQIYTRGGEQGAAFQSDYIELFNRGNTSIDINGFALQLTTSNNTVTAITF
                     SSSQGIMVGPGKYVLLEMARGTSGQPLPAPDFPSIGSFTPNLSPTAGYVALYRRSPTV
                     FFGGCPSGDDLLDMVGYGAGAACFEGGAPAPAPSEPTTALVRDRGGCDDFNTNALDLR
                     VGAANPRNRQTSAALCTITPGPSTIEFAATQFDAAESDGRAAITVTRVGETVAPATVE
                     FIVADGAANDRQDYTTAWGTLRFEPGETSKTFRILLTDDAAQEDNETVQMALANPTGL
                     ATLGTRALASLVIQDNDFGSAAPNPDDDPTFFARQHYHDFLNREPDASGLAFWSNQIN
                     ECGTNAACREVRRINTSAAFFLSIEFQETGFLVHRIYRAAFSESAARPRGLPRYREFV
                     RDSQTISRGVVVGEGDDWRLRLDTNQREFVKEFVERAEFKALYPATMSAAEYVDALNR
                     NAGGFITSPLREQMIFNLQSGAFDRAEVLRIFAENRAFTAAQKNPAFVLMQYFGYLRR
                     NPDDAPEAGLNFDGYDFWLAKLNQFGGDFQRAEMVKAFITSAEYRQRFAAAAP"
     gene            3584..>4271
                     /locus_tag="VF240_15525"
     CDS             3584..>4271
                     /locus_tag="VF240_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176675.1"
                     /translation="MLGLLLPAGNLFKDLMPAAHAAPLVANPTSTIFINEIHYDNTGT
                     DAGEFVEIAGPAGTSLTGYSIVRYNGSDGAVYTTPAATTTLPATIPSQQGGYGTATIS
                     YPANGIQNGAPDGLALVGPGNVLIQVLCYEGTFTAVGGAAAGQTCTDIGASEVGTEAL
                     GLSLQLTGTGTTYGEFTWTGPVTASAGAVNAGQSFGAPTPTPTPTPTPTPEPTPTPTP
                     TPEPTPTPTPT"
BASE COUNT          748 a         1551 c         1265 g          707 t
ORIGIN      
        1 ggacttatca agattatgaa cggtgaaatt tttatcctcg gcggcaagcg cacgccgatg
       61 tgctcctacg tcggggcgct caaggacgtg aaggcgattg acctcggagc tacggccgcg
      121 cgcggcgcac tcgaagccac gggtgtggcg gccgaagaga tcgatcacac cgtcttcggc
      181 aacgccttgc agacctcggg cgacgccatc tacggcgcgc gccacgtcgc cttgaaggcc
      241 ggcgtgcccg ccgagcggcc cgccttgacc gtcaaccgcc tgtgcggcag cggcatccag
      301 tcaatcgtct cgggcgcgca catgattctg ctgggcgagg ccgagacggc tttggtcggc
      361 ggcatggaat cgatgtcgat ggccccgcac gccatctacg gcgcgcgcgc cggcttcggt
      421 ctcggtcagg gcaagctcga agacaccctg atggtcgcgc tcttagacac ctactgcaac
      481 acgacgatgg ccggcacggc cgagaacctc gcgcgccgct acgaaatctc gcgcgaggag
      541 caggacaagt atgccctgcg ctcgcagcag gaggccgcgc gcgcgcaagg ccagggcgtc
      601 ttcgctgagg agatcgtccc cgtcgaggtc aagacgcgca agggcgtgaa gacaattgac
      661 acggacgatc acctgcgtcc cgacacgacg ctcgaaattt tggcgaagct cccgaccgcc
      721 tttagcaagg acggatttgt gacggccggc aacgcctccg gcatcgtgga cggcgcggcg
      781 gcgctcgtca tcgccggcgc ggagtacgtc cgtcgcaagg gcgcgaagcc ggcggcccgg
      841 atcgtgagct gggcctacgc cggcgtcgag ccggaagtga tgggcatcgg ccccgtcccg
      901 gcgattcgca aggcgctgga gaaggcgaac ctgaagctcg aagagatcga cttagtcgag
      961 gtcaacgagg cgttcgccgg gcagtacctc gccgtcgaga aggagttggg gctggagcgc
     1021 gcgcgcacga acgtcaacgg cggcgccatc gccctcggcc acccgctcgg cgcttcgggc
     1081 acgcgcctcg tcctcacggc gctccttgag ctgcaacgcc gagggggtcg ctacggtctg
     1141 gcgtcggcct gcatcggcgg cggtcagggc atcgccatcg tcttcgagcg catctaaaac
     1201 gcgcaaaaga accgccgaag gcgcgcgcgt ccgttgggac ttttgagtac ttcgacgcgc
     1261 gcgccttgcc gcgtcatccc gaacgacaat ttgttagaga ccgagctatt tattaacgga
     1321 gtatatttgt gtccgcattt cctcgcgccc ccgaaatttt cctccccaat cgtcgtcatc
     1381 ttctaacgcc cgccgcgctc gcggcctgcg ccctttttgt ttgcctcatc acgtctgccg
     1441 tgacggcgcg ggcgcaggag tccttcgccc ccaacatcgt catcagccaa atctacacgc
     1501 gcggcggcga gcagggcgcg gccttccaga gcgactacat tgagctgttc aaccgcggga
     1561 acacgagcat cgacatcaac ggcttcgcgc tccagttgac gacgagcaac aacaccgtca
     1621 cggcaatcac gttctcctcc tcgcagggca tcatggtcgg gccgggcaag tacgtcctct
     1681 tggagatggc ccgcggcacg tcggggcaac cgctgcccgc gcccgacttc ccctccattg
     1741 gttccttcac cccgaacctc agccccaccg ccggttacgt cgcgctctac cggcgctcgc
     1801 ccacggtgtt cttcggcggc tgtccttcgg gcgacgacct cctcgacatg gttggctacg
     1861 gcgcgggggc cgcctgcttc gaaggcggcg ccccggcccc cgcgccctcc gagccgacga
     1921 ccgcgctcgt gcgcgaccgc ggcgggtgcg acgacttcaa caccaacgcc ctcgatctgc
     1981 gcgtcggcgc agctaacccg cgcaaccgcc agacgagcgc cgcgctctgc accatcacgc
     2041 cggggccgag caccatcgag ttcgcggcga cgcagttcga cgcggccgag tcggacggcc
     2101 gcgccgccat caccgtcacg cgcgtgggtg agacggtcgc cccggccacg gtcgagttca
     2161 tcgtcgccga cggcgcggcc aacgaccggc aggactacac gaccgcatgg ggcacgctgc
     2221 gcttcgagcc gggcgagacc tcgaagacct tccgtattct cctcacggac gacgccgcac
     2281 aggaagacaa cgagaccgtc cagatggcgc tcgccaaccc gaccggcctc gccacgctcg
     2341 gcacgcgcgc gctcgcctcg ctcgtcatcc aggataacga cttcggctcc gccgccccga
     2401 atcccgacga cgacccgacc ttcttcgcgc gccagcacta tcacgacttc ctcaaccgcg
     2461 agccggacgc ctcgggcctc gccttctggt cgaaccagat caacgagtgc ggcacgaacg
     2521 ccgcctgtcg cgaggtgcgc cgcatcaaca cgagcgcggc cttcttcctc tccatcgaat
     2581 ttcaggagac gggcttcctc gtccaccgca tctaccgcgc cgccttctct gagagcgccg
     2641 cgcggcctcg cggcctgccg cgctaccgcg agttcgtccg cgactcgcag accatcagcc
     2701 gcggcgtcgt ggtcggcgag ggcgacgact ggcgtctccg actcgacaca aatcagcgcg
     2761 agttcgtcaa agagttcgtc gagcgcgccg agttcaaggc gctctacccg gcgacgatgt
     2821 cggcggctga gtatgttgac gcgctcaacc gcaacgccgg gggcttcatc acgtcgccgc
     2881 tgcgcgagca gatgatcttc aacctccagt cgggcgcctt cgaccgcgcc gaggtgctgc
     2941 gcatcttcgc cgagaaccgc gcgttcaccg ccgcccagaa gaatccggcg ttcgtcctca
     3001 tgcaatactt cggctacctg cgccgcaacc cggacgacgc gccagaagcc ggtctcaact
     3061 tcgacggcta cgacttctgg ctcgcgaagc tcaaccagtt tggcggcgac ttccagcggg
     3121 ccgagatggt caaggcgttc atcacgagcg cggagtaccg gcagcgtttc gccgcggccg
     3181 cgccctagcg aaaagcaggg atgaagtgaa gacaagatgc cttctttcat ccctcatccc
     3241 tcatccctgc ttttccgcga gcggcgccgc atttaacatt taactgatat gtaagcgtct
     3301 attaaccggc ccgtgcaacc ggcggctgta tacactcatc tagtacaggt cacatttcca
     3361 ggggaaacgg cttgcgggtt gactcctaat tttttttcag gtacaagcca agcgtcgcct
     3421 cggttcggtc atccacgccc acgcgtcctc cggcagtaac ggacgttggc cttaacaacc
     3481 acccatccaa gagaaaggat ctgctcccgt gtctttagcc caccactcaa ggcgagcgtc
     3541 tgtctccgtc cgccgtcaac tcgtttcgtc attactaacc ctgatgctcg gcctcctcct
     3601 cccggccggc aacctcttta aagacctgat gcccgcggcg cacgccgccc ccctagttgc
     3661 caaccccacg tccacgatct tcatcaacga gattcactac gataataccg gcactgacgc
     3721 cggcgaattc gtcgagatcg ccggccccgc cggaaccagc ctgaccggct attccatcgt
     3781 tcgctacaat ggcagcgacg gcgcggttta caccaccccc gctgccacca ctacccttcc
     3841 ggcaacgatc ccgtcacagc agggcggcta tggaactgct accatctcct acccggcgaa
     3901 tggcatccag aacggcgcgc cggacggcct ggcccttgtc gggccgggca acgtcctcat
     3961 tcaggtcctc tgctacgagg gcaccttcac ggcggtcggc ggcgcggcgg ccgggcagac
     4021 ctgcaccgac atcggtgctt cggaggtcgg cacggaagct ctcggcctct cgctccagct
     4081 cacgggcacc ggaaccacct acggtgagtt tacgtggacg gggccggtga ccgcctcggc
     4141 cggcgcggtc aacgcgggcc agtccttcgg cgccccgacc cccacgccca cgcccacgcc
     4201 gactccgact cccgaaccca caccgacccc gactccgacc cctgaaccta ctccgacgcc
     4261 cacgccaacg c
//
LOCUS       DASVES010000380         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_63617, whole genome shotgun sequence.
ACCESSION   DASVES010000380 DASVES010000000
VERSION     DASVES010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63617"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..2303
                     /locus_tag="VF240_15530"
     CDS             384..2303
                     /locus_tag="VF240_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7176676.1"
                     /translation="MRLPHTKHHPKPLAACALLLVCLFACDSAQAQTAPPKVSKVEPP
                     SWWALHTINPVRLLVRGTNLHGARVTSQSAELEAFGALTNPAGTYLFVNVSIRPTARP
                     GDYPLTVETPSGRASIPFRINERLDASKNFQGITTDDVLYLIMPDRFANGDTSNDAPA
                     DAPPEANSRANPRAWHGGDLRGIIDRLPYLKELGVTALWLNPWQDNWNGLNRCDKPWC
                     PSTYYHGYHTVDYYGVEDRFGTLATLRELVDKAHAVGIKVVHDQVANHVVVKHPWAAD
                     PPLQNWFHGTVAKHALNPFRGDTLLSPHAPASERQPTLDGWFADDMPDMNQEEPEVAR
                     YEIQNALWWVGVTGMDGIRQDTIQYMPRAFIRDLSRALHAEHPRMWMVGEVFDRDPAH
                     VSFFLGGRLGWDGIDTELDSVFDFPVWQASLDVFTGKKPMRALRDALKYDALYPDAHR
                     LTTMANNHDVKRFMSLEGATPEGSMLHVAFTLSIRGAPQLYYGEEIAMPGGDDPDNRR
                     DFPGGFPRDPRNAFTRAGRTPAEQRMFEWTRDWIRLRREHPAMRRGRQVDLFYDDDAY
                     AFARAGEGETVVLVFNRSAAPKQITLPAAALEARDGALLLPLLVAKDTVTVAAGKATL
                     TAPARTAVAYKVSDK"
     gene            2419..>2921
                     /locus_tag="VF240_15535"
     CDS             2419..>2921
                     /locus_tag="VF240_15535"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7176677.1"
                     /translation="MSFPRTSGVILHPTSLPGRYGVGDLGDEAYKFIDFLASAGQHLW
                     QVLPLGPTGYGNSPYQCFSAFAGNTLLVSPERLLADGLLGAADVADAPEFVEGEVEYG
                     RAIEFKNALLRKAFENFRAGTEPSIRSEFDSFRHDAADWLDDYALYRALKRERGEIAW
                     TEWEPALV"
BASE COUNT          510 a         1042 c          912 g          457 t
ORIGIN      
        1 gcgcccgaca tgacggacgc gcgctggcac gcgcgccgcg gcgcgcgtcg catgacgcag
       61 tcggtcgcgc gcggacgcgg gcagatgccg gccttcgccg gcaagctcac gaaggctgag
      121 atcgccgcga ccatcgctta cgtccgcacg ctccgaaagt aagcgcgggc agtcgccggc
      181 gaaagatttt tgttccctac agggcgcagt ccgatttaga atcggcgcga gtcatacccc
      241 cgagttttag ctgtagcaaa gtagagacgt tcgtacacca cttcggacga attggagcga
      301 gtgtgcgtgg cgggcgaccg gttggccggg caggacacta ttgcccggcg gcgcaccggc
      361 ccgcaacgca cgcctccgcc ttcatgagac ttccacacac gaaacaccac ccgaagccgc
      421 ttgccgcgtg cgcgctcctg ctcgtctgcc ttttcgcgtg tgactcggca caggcacaga
      481 cggccccgcc gaaagtgtcc aaagtcgagc cgccttcgtg gtgggcgctt cacacgatca
      541 acccggtgcg tctgctcgtg cgcggcacga acctgcacgg ggcgcgggtc acttcgcaga
      601 gcgcggagct tgaggcgttc ggcgcgctca cgaatccggc gggcacatac ctcttcgtca
      661 acgtttcaat ccgccccacg gcgcgccccg gcgactatcc gctgacggtt gagacgccct
      721 cggggcgcgc gtccattcct ttccgcatca acgagaggct cgacgcgtcg aaaaactttc
      781 agggcatcac gacggacgac gtgctctacc tcatcatgcc cgaccgcttc gcgaacggcg
      841 acacgtcgaa cgacgcgccc gccgacgcgc cgcccgaggc gaacagccgc gcaaacccgc
      901 gcgcgtggca cggcggcgac ctgcgcggca tcatcgaccg cctgccttat ctcaaggaac
      961 tcggcgtcac cgccctctgg ctcaacccgt ggcaggacaa ctggaacggt ctcaaccgct
     1021 gcgacaagcc ttggtgcccg agcacgtatt accacggtta tcacaccgtg gactactacg
     1081 gcgtcgaaga ccgcttcggc acgttggcga cgctgcgtga gctggtcgat aaggcgcacg
     1141 ctgtcggtat caaggtcgtc cacgatcagg tggctaacca cgtcgtcgtc aagcacccgt
     1201 gggccgccga cccgccgctc caaaactggt tccacggcac cgtcgccaag cacgccctca
     1261 accccttccg cggcgacacg ctgctctcgc cccacgcgcc ggcaagcgag cgccagccga
     1321 cactcgacgg gtggtttgcc gacgacatgc cggacatgaa tcaggaggag ccggaggtgg
     1381 cgcgctacga gatacagaac gcgctctggt gggtcggcgt caccggcatg gacggcatcc
     1441 ggcaggacac catccagtac atgccgcgcg ccttcatccg cgacctctcg cgtgcgctcc
     1501 acgccgagca cccgcgcatg tggatggtcg gcgaggtctt cgaccgcgac ccggcgcacg
     1561 tcagtttctt cctcggcggc cggctgggct gggacggcat cgacacggaa ctcgattccg
     1621 tcttcgactt ccccgtgtgg caggcgtcgc tcgatgtgtt cacgggcaag aagccgatgc
     1681 gggcgttgcg cgacgcgctg aagtacgacg cgctctaccc ggacgcgcac cgcctgacga
     1741 cgatggccaa caaccacgac gtaaagcgtt tcatgtcgct cgaaggagcc acgcccgaag
     1801 gctcgatgct tcatgtcgcc ttcaccctct ccattcgcgg cgccccgcag ctctactacg
     1861 gcgaggagat cgccatgccc ggcggcgacg accccgacaa ccggcgcgac ttccccggcg
     1921 gcttcccgcg cgacccgcgc aacgccttca cgcgagcagg gcgcacgccc gccgagcagc
     1981 gcatgttcga gtggacgcgc gactggatac gcctgcgccg cgagcacccc gccatgcgcc
     2041 gcggccggca agtcgatctc ttctatgacg acgacgcata cgccttcgcc cgcgcgggcg
     2101 agggcgagac ggtagtgctc gtcttcaacc gctccgccgc gccgaagcag atcacgctgc
     2161 cagcggcagc ccttgaggca cgagacggcg cgcttctgct gcccctgctc gtcgccaaag
     2221 acaccgtgac cgtcgcggcg gggaaggcaa cgctgacggc gccggcgcgc acagcggtcg
     2281 cgtacaaggt gagtgataag tgagaacagt accgcgagcg gtagcgagcg ggtctgcctg
     2341 cgacaagagg cccgcacctc gcacgcgata cccgctcgct accgctcgcg gtactgtttc
     2401 atcgcttgga gatagtcatt gagcttcccg agaacgagcg gcgtcatcct gcacccgacg
     2461 agtttgccgg ggcgctacgg cgtcggcgac ctcggcgacg aagcatataa gttcatcgac
     2521 tttctcgctt ccgccggcca acatctctgg caggtgctcc cgcttgggcc gaccggctac
     2581 ggcaattcgc cctatcagtg cttctcggcc ttcgccggca acaccctgct cgtcagcccc
     2641 gaacgcctcc tcgccgacgg actgctcggc gcggccgacg tggccgacgc gcccgagttc
     2701 gtcgaaggcg aagtcgagta cggccgcgcc atcgagttca agaacgcgct gctcagaaag
     2761 gcgttcgaga atttccgcgc cggcacggag ccatcgattc gctcagagtt cgactccttc
     2821 cgtcacgacg cggccgactg gctcgacgac tacgcgctct atcgcgccct caagcgcgag
     2881 cgcggcgaga ttgcgtggac ggagtgggag ccggcgctcg t
//
LOCUS       DASVES010000381        10916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1262252, whole genome shotgun sequence.
ACCESSION   DASVES010000381 DASVES010000000
VERSION     DASVES010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262252"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VF240_15540"
     CDS             <1..1473
                     /locus_tag="VF240_15540"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7176678.1"
                     /translation="DVGKGLSQVGASANANADAATHMDAHAATRADAVASSTSSHVSS
                     THLSSTHVTVIPRARVARGRFEPAKALDALAGVVGPQNVSLRGESVVVAPCTTAEVRE
                     VLRLTGGTGWPVVPAGGETWTDAGNPLACAGRVVVNTTRLTGVVEHEPADLVATARAG
                     TTLVHFNGALALARQWLPLDPPDDGRATLGGVAATGTGGAQSLAYSPPRSHVLGMTVA
                     LADARIIHVGGRVVKNVAGYDLCKLFVGSHGTLGLILDVTFKLRPRPRREATVVAQAR
                     DTRTLLEAGRAIIAANLSPVAVELLSAQVVAGAGIETAGGSAGLLLRFAGTDEAVAWQ
                     TAKAVETIGARSRLVDVNVLDDDAAVWAGLQSAALSLPARIVLRARVPSAGLPDVLDA
                     VSNVRAGDGSRLCWHAGVGDGRLRLFDDEERTDVEHAAEVERLRVAARRLGGSLVVER
                     APVAVKAIVDAWGVSDSTGALMRRVKSQLDPDGVFSPGRF"
     gene            1575..2957
                     /locus_tag="VF240_15545"
     CDS             1575..2957
                     /locus_tag="VF240_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX7176679.1"
                     /translation="MSHETQLGGALAANGEKILACIHCGLCLEACPTYVHTGDENDGP
                     RGRIYLMRAVEEGQLPVESASFRTHISRCLGCRACESACPAGVEYGHLLEAARSDIAA
                     VAPPRGATARLLNFALRHVWTHPRRLRLAFKLARVFRDCGVPRVLRALGAARLLPSRT
                     AFALALLEASAPRSLSLGDRLDDKSSDRAGGRSTPRVDSATEPASPARKALLFTGCVM
                     EGLFARVNRATGRVLAANACAAVAPDEQVCCGALHAHAGDLEGARRLARRNIDAFADE
                     AERAIVTNAGGCGAMLASYDKLFAEGDTYAERARQFAARIRDVSQQLEAVGQREGAPL
                     GDAGAVTYDASCHLIHGQRAFAAPLKILAAIPRLRFVPLEGSDVCCGGAGVYNLLEVE
                     MSERVLGEKLARVGDTGARLLATGNPGCHMQIGAGALLAGSDLRVCHPVELLDESYRR
                     AGFYAADESV"
     gene            complement(2969..4195)
                     /gene="nagA"
                     /locus_tag="VF240_15550"
     CDS             complement(2969..4195)
                     /gene="nagA"
                     /locus_tag="VF240_15550"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX7176680.1"
                     /translation="MSEKKKRDGHLLLRGASITGARGVLSEGCLLIEQGRIKRVFGPE
                     EPLPAHDVETIDLEGLTLLPGFIDIHIHGALGVDVNEADANGLGRVSRFLVARGVTSW
                     LPTLVPAPDEDYASAARSVEQLMREQEHETEAESRAAEEPGARALGLHYEGPFVSQHQ
                     CGALRTQFFRSYAEPSDLERLALVGRADAVHMTTLAPEIDGGVELVRELVARDWVVSI
                     GHTRAGVDVLEQARAAGARHMTHFMNAMPQFHHRTPGPVGWGLLADDVTCDLIADGVH
                     CEALALRLAVRCKTPERIALISDAVAPTGLGEGEHRVWGETIRVEGGRTSNERGSIAG
                     SVISMLDAVRLCLSLGMTTAEVARMAAHVPARVLGIDADCGSIESGKRADLVALDADF
                     NVRLTVVGGRVAFRAD"
     gene            4347..5528
                     /locus_tag="VF240_15555"
     CDS             4347..5528
                     /locus_tag="VF240_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176681.1"
                     /translation="MFVLTASHKIITARGLLACGLLLLPIGTTAQTPPPNDCSGATPQ
                     LAAARAAEERSDWSAALSNYQTALEHAPRCVELLINLGATHNRLGQPEQAIVTFQRAA
                     TLNPNLAVAHLNLGITFFRARRYEDAGAALARAVEIEQSNVQARQMLALTLIAREQFT
                     EAAAQLEWVLAREESNRGVLLALGECYLRLKRYREAAERLGRLVQLDPNSAAARLRHG
                     EALDNSGESELALGAFQRAVQLDARLSEAHYALGYAFLKRGNLAEAASAFEAELRLNP
                     DMLLARYHLGKVYLQQERLAEATESLTRVVDASPEFRPGLEALGQALLKQKEAARAVV
                     MLERAVKLGPDWPDGHALLGRAYMAAGRRAEAGRAFAMAQQLAARERKRLEEKVDKQK
                     P"
     gene            complement(5644..9123)
                     /locus_tag="VF240_15560"
     CDS             complement(5644..9123)
                     /locus_tag="VF240_15560"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7176682.1"
                     /translation="MFKRIIALVACALTLSATTAFAQEVTGSILGTVTDTAGAAVPNA
                     EVTLTSTNTREERKIQTDEDGNYTFTQLRPGVYDIAVRTQGFKEHVSQGFEVNVNDRK
                     TLNFTLEAGAVSETITVTSEAPLIQTTPTVGDVVENRRVVEIPLNNRNFMQLLTLIPG
                     VTSSGSSEIGIGLTNVVDFSINGTRRNSINFLVDGVTNTDVGSNVTLLSVPTVDSIQE
                     FRVITSVPSAEFGRSGGGVVNIITRGGGSDFHGTLYEFVRNDRLNANSFFNNANGTFG
                     PNDATVLAGLATAGEPRTPRPKLRYNNFGYLVSGPIFIPGHYNTNRDKTFFLFSHEFR
                     RIIRAPSLASAITVPSLLERQGNFSEAGNLPIFDPLTNLQFPGNIIPSARINPAAVAL
                     LSLYPEPNIPSTTAGRAPNRYAVVTPNIQNTRQETIRIDHNFSSTHRLTGRYTHDLSA
                     TRELGGLFFGATIPDIATTNTNVPGEVLAIGLTSTFGPNIVNEATFTFSGNKITSDLI
                     GRYNSDLSVPHNEIFEQNNSGLPPIINITGQPTIGSGQLFDVKYRNWNPKNNVTMIRG
                     SHTFKFGGEVSFESKDENAASNTQGTYGFTGITTRVGNNAGIGLADFLLGRAASYAEP
                     ERDVIEHLRFGRTEFYFQDTWKVRPNFQLDYGIRYYRFRQPYDTEDVLATFLPEFYDR
                     SRAPTCANVSCTLFTLSTFDLTNGFAYAGQNSPFGRQVQKSDKNDWGPRIGFAWDPRG
                     EGKMVLRGGYGVYYDQALVGIVEQNSFTTPPFNNSISLTGTFASPVPFGSPAPVAATT
                     RGNLGTVNATTDPFVTPIVQQWSLTWQQEPFRNTVFEVGYAGSAGNHLIRPVDINAPT
                     PTEILNAASGVAGCDPALLASNNPANCINLARPFRGFGTITDRQTTATSRYHGLISSF
                     RMRTAKGLTAQMSYTWSKNITDATNDRDAIDVPQIRTDFTLERAVSRLDRTHVFVASY
                     VYEVPTFGSGFAKTTAGRLLLSGWEIAGITTAQTGLPLNRVVQGSATVPARGSRPNLI
                     SDPTQNIPVNPAGGIPYAFNPLAFQTTLSGQIGNSPRSPFRFPSQFFTDVNLSKNLIF
                     TENIRVQLRAEFFNIFNKTIFNDVFQTIPDRLPTDAAFNSVQNLININSQFGQFFSTR
                     DPRQVQLGLKFYF"
     gene            complement(9253..10878)
                     /locus_tag="VF240_15565"
     CDS             complement(9253..10878)
                     /locus_tag="VF240_15565"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX7176683.1"
                     /translation="MKRHLRRAALAAAVAALLFSQQPARLRAATGAASEARYDLVITN
                     ARVVDGAGNPWFRADVGVREGRIARVGRIAPEEAARVIDARGQILAPGFIDVHAHVED
                     IYNLPEAENFVRMGVTTLVSGNCGGSAVNVSEYLDGVRSKPLAVNLATLVGHGSVRAA
                     VMGTANREPSAEELQKMEGLVEQAMRDGAVGLSTGLIYVPGTYAKTGEVVALARIAAR
                     HGGVYATHMRNEGERVLDAIRESIHIGEQAGLPVEISHFKISNKRWWGRTGETLGLVR
                     EARARGLQVTVDQYAYTASSTSLDSRIPSWVLEGGREEGKKRLADKPTRARVVKEMRD
                     GLKRSGFKDYSYAFVASHRADPALNGKNIAEITKQARGRADIGSQIEQILSMYEAGGA
                     SMVYHSMGEEDVRRIMREPFTMIASDSGVRRHGEGVPHPRGYGNNARVLGRYVREQGV
                     IPLEDAVRKMTSLPAQTFGLFDRGLVREGMAADLVVFDEKTVADLATFDQPHQYAAGI
                     IHVFVNGESVVADGQLTAARPGAAIRHAPRGKM"
BASE COUNT         1730 a         3465 c         3798 g         1923 t
ORIGIN      
        1 gatgttggta aaggtctgtc gcaggtgggc gcgtcggcga acgcgaacgc cgacgccgcg
       61 acgcacatgg acgcccacgc cgcgacgcgc gctgacgccg tcgcttcttc aacttcaagt
      121 cacgtctctt caactcatct atcttcaaca cacgtcaccg ttatcccgcg cgcacgcgtt
      181 gcgcggggcc gtttcgagcc ggcgaaggcg ctcgatgcgc tggccggagt cgtagggccg
      241 cagaacgtct cgctcagggg tgagtcggtc gtggtcgcgc cgtgtacgac ggcggaagta
      301 cgcgaagttt tgaggctgac cgggggcacg ggctggccgg tcgtgcccgc tggcggcgag
      361 acttggactg acgccggtaa cccgctcgcg tgcgccggcc gcgtcgttgt caacacaacg
      421 cgcctcaccg gcgtcgtcga gcacgagccg gcagacctcg tcgccacggc ccgcgccggc
      481 acgacgctcg tccactttaa cggcgcactc gcgctcgcgc gacaatggct cccactcgac
      541 ccgcccgacg acgggcgcgc gacgctcggc ggcgtcgccg cgaccggcac gggcggcgca
      601 cagtctctcg cctactcccc gccgcgctcg cacgtcctcg gcatgaccgt ggcgctcgcc
      661 gacgctcgca tcattcacgt cggcggccgc gtggtgaaaa acgtggccgg ctacgacctc
      721 tgcaagctgt tcgtcggcag ccacggcaca ctcggtctga tactcgacgt gaccttcaaa
      781 ctccgcccgc gcccgcgccg cgaggcgacc gtcgtcgcgc aggctcgcga cacgcggacg
      841 cttctcgaag ccggccgcgc aatcatcgct gcgaacctct cgcccgtcgc cgtcgagctt
      901 ctctcggcgc aggtagtggc cggcgccggg atcgaaaccg ccggcgggag cgccggcctg
      961 ctcctgcgtt tcgcgggcac tgacgaggcc gtcgcgtggc agaccgcgaa ggccgtcgag
     1021 actatcggcg cgcgcagcag gctcgtcgat gttaatgtgc tcgacgacga cgcggccgtc
     1081 tgggcgggcc tccagtccgc cgctctgagc ctgccggcgc gcatcgtgtt gcgcgcgcgc
     1141 gtgccttccg cggggttgcc tgatgtgctc gacgccgtat cgaacgtgcg ggcgggcgat
     1201 ggttcaagat tgtgttggca cgcaggggtc ggcgacggcc ggctccgatt gttcgacgac
     1261 gaggaaagga cggacgtgga gcacgcggcg gaggtcgaac gtctccgcgt agctgctcgg
     1321 cggctcggcg gctcactcgt cgtcgagcgc gcgcccgtgg cggtgaaggc catcgtggac
     1381 gcgtggggtg ttagtgattc gaccggcgcg ctcatgcggc gcgtcaagtc gcaactcgac
     1441 cccgacggag ttttcagtcc ggggcgcttc taacagtacc gcgagcggta gcgagcgggt
     1501 ttgacgcgag cgagacgggc ctctggtcga tggcacaccc gctcgctacc gctcgcggta
     1561 ctgttcaccc aagcatgtca cacgagacac aactcggcgg cgcgctcgcg gcgaacgggg
     1621 agaagattct ggcctgcatc cactgcggcc tgtgtctgga ggcttgcccg acttacgtcc
     1681 acacgggcga cgagaacgac gggccgcgcg ggcgcattta cctgatgcga gccgtcgagg
     1741 aggggcaact ccccgtagag tccgcctcct tccgcacgca catcagccgt tgcctgggct
     1801 gtcgcgcgtg cgagagcgcg tgcccggcgg gcgtcgagta cgggcacctg ctcgaagccg
     1861 cgcgctccga catcgccgcc gtcgcgccgc cgcgtggggc cacggcgcgc ctgctcaact
     1921 tcgcgctccg acacgtctgg acgcatccgc gccggctgcg actcgcgttc aaactcgcgc
     1981 gcgtcttccg cgactgcggc gtcccgcgtg tgctgcgcgc gttgggcgcg gcgcgtctcc
     2041 ttccgtcgcg caccgcgttc gcgctggcgc tcctcgaagc gtccgctccc agatcgttga
     2101 gcttaggaga tcgtttagac gataaatcat ccgaccgcgc cggcggtcgc agcactccga
     2161 gggtggacag tgcgacggag ccggcgtcgc cggcaaggaa ggcgctcttg ttcacgggct
     2221 gtgtgatgga aggattgttc gcgcgtgtga atcgcgcgac ggggcgcgtg ctcgcggcga
     2281 acgcctgcgc ggccgtagcg ccggacgaac aggtctgctg cggggcgctc cacgcgcatg
     2341 ccggcgacct cgaaggcgca cgccggctcg cccgccgcaa catcgatgcc ttcgccgacg
     2401 aagcggagcg ggccatcgtc accaacgccg gcggctgcgg cgcgatgctc gcctcgtatg
     2461 acaaactctt cgccgaaggc gacacttacg cggagcgcgc ccgtcagttc gccgcacgca
     2521 tccgcgacgt gagtcaacaa ctcgaagccg tgggccagcg cgagggcgca ccgctcggcg
     2581 acgcgggggc cgtgacctac gacgcctcct gccatctcat ccacggccag cgcgcctttg
     2641 ccgccccgct gaaaatactc gccgcgattc cccgccttcg cttcgtcccc ctcgaaggca
     2701 gcgacgtgtg ctgcggcggg gccggcgtct acaacctgct cgaagtggag atgtcggagc
     2761 gcgtgctcgg cgagaaactg gcgcgcgtcg gcgacaccgg cgcgcgcctg ctcgccacgg
     2821 gcaatccggg ttgccacatg cagatcggcg ccggcgcact cttggccggg tcagacctgc
     2881 gcgtctgtca ccccgtcgaa ctcctcgacg agtcctatcg gcgcgcgggt ttctacgccg
     2941 ccgacgagtc agtgtaatcc ttgaaagttt agtccgcgcg gaaggccacg cgcccgccga
     3001 cgacggttag cctgacgttg aaatccgcgt cgagcgcgac caagtcggcg cgcttgcccg
     3061 actctatgga gccgcagtcg gcgtcgatgc cgagcacgcg cgccgggacg tgcgcggcca
     3121 tgcgggcgac ttccgcagtc gtcatgccga gtgaaagaca gagacgcacg gcgtcgagca
     3181 tggagatgac cgagccggcg atactcccgc gctcgttgct tgtgcgtccg ccctcgacgc
     3241 gaatcgtctc gccccagacg cggtgctcgc cttcgcccag ccccgtcggc gccaccgcgt
     3301 cgctgatgag ggcgatgcgc tccggcgtct tgcaacgcac ggcgagacgc agggccagcg
     3361 cttcgcagtg aactccatcg gcgatgaggt cgcaggtcac atcgtcggcg agcaagcccc
     3421 agccgacggg gccgggcgtg cggtggtgaa actgcggcat ggcgttcatg aagtgcgtca
     3481 tgtgacgcgc cccggccgcg cgcgcttgtt cgagtacatc gaccccggcg cgtgtatggc
     3541 cgatggagac gacccagtcg cgcgcgacga gttctcgcac cagctccacg ccgccgtcga
     3601 tctcgggcgc gagcgtcgtc atatgcacgg cgtcggcgcg gccgacgagg gcgaggcgtt
     3661 cgaggtctga aggctcggcg taggagcgga agaactgtgt gcggagcgcg ccgcactggt
     3721 gttggctgac gaacggcccc tcgtagtgga ggccgagggc gcgcgcgccg ggttcttccg
     3781 ccgcgcggga ttccgcttcc gtctcgtgct cctgctcgcg catcaactgc tcgacggagc
     3841 gcgccgcgct cgcgtagtct tcgtcgggcg ccgggacgag cgtcggaagc caggaggtga
     3901 cgccgcgcgc aacgaggaag cgcgacacgc ggccgaggcc gttggcgtca gcttcgttca
     3961 cgtcaacgcc caaagcgccg tggatgtgga tgtcgatgaa gccggggaga agcgtcaacc
     4021 cctccagatc aatggtctca acgtcgtgcg cggggagagg ttcttcaggc ccgaacacac
     4081 gtttgatcct cccctgctcg atcaggagac atccttccga gaggacgccg cgcgcgcctg
     4141 taatggacgc tccacgcaga agcaaatgac cgtcacgttt cttcttctca ctcataatct
     4201 cgatgccttc attcacatgc gtctcaagtc aactcgcgaa caacactcca acagagagcg
     4261 gccaatcgta aaatccaacg cttggctaat caagttgcat cctctatgat tttgtaagtg
     4321 ttacccgctc tggcaacgat tccatcatgt tcgtcctgac agcttcgcac aagattatca
     4381 ccgctcgcgg tctcctggca tgcggccttc tgctgctccc gattgggacg acggcacaga
     4441 cgccgccgcc caatgactgc tccggcgcga ctccacagct tgccgcggcg cgcgcggccg
     4501 aggagcgctc agactggagc gccgcgcttt ccaactatca aacggccctc gaacacgcgc
     4561 cccgctgcgt cgaacttttg ataaatctcg gcgcgactca caatcgcctc ggccagccgg
     4621 agcaggccat tgtgacattt caacgcgccg cgacgctcaa tccgaacctc gctgtggcgc
     4681 acctcaactt aggcattacg tttttccgcg cgcgccgcta cgaggacgcc ggcgcggcgc
     4741 tcgcacgcgc ggtcgagatc gaacagtcca acgtgcaggc gcgacaaatg ctggcgctca
     4801 cactgatcgc gcgcgagcag ttcaccgaag ccgccgcgca gctcgaatgg gtgctggcgc
     4861 gcgaggagtc gaatcgtggg gtgctgctgg cgctcggcga atgctatctg cgtctgaagc
     4921 gataccggga ggctgccgag cgcctgggcc gtctcgtgca gttagaccca aactcggcgg
     4981 cggcgcggct ccgccacggc gaggcgctcg acaactccgg cgaatccgag ttggccctcg
     5041 gcgctttcca acgggccgtt cagctcgacg cgcgtttgtc cgaggctcac tatgccctcg
     5101 gctacgcctt cctcaagcgg ggcaatctcg ccgaggcggc cagcgccttc gaggccgagc
     5161 ttcggctcaa ccctgacatg ttgctggcac gctaccatct cggcaaggtt tatctgcaac
     5221 aagagcggct cgccgaggcg acggaatcgt tgacccgcgt ggtcgatgcg tcgccggagt
     5281 tccgccccgg tcttgaggca ctgggccaag cgcttttgaa acagaaggag gcggcgcgcg
     5341 ccgtcgtcat gctcgaacgt gcggtcaaac tcggccccga ctggcccgac ggccacgcgc
     5401 ttctcgggcg tgcctacatg gccgccggac gccgggcgga ggccgggcgg gcgttcgcca
     5461 tggcccagca actggccgcg cgcgagcgca agagactcga agagaaggta gacaagcaga
     5521 agccctgacc tcgacagtac cgaggtcagg gctttcagct tgctcagtct taacggtcgt
     5581 ggtacgaggg ccgactcggc gggagtcggc cctcgaactg taagcgcacc gaggacgatt
     5641 cgcctagaag tagaacttca agccgagctg aacctgacgc gggtcgcggg tcgagaagaa
     5701 ctgaccgaac tgcgagttga tgttgatcag gttctggacg ctattgaagg ccgcgtcggt
     5761 cggcagccga tcagggatcg tctggaacac gtcgttgaag atcgtcttgt tgaagatgtt
     5821 gaagaactcc gcgcgcagtt gcacacggat gttctccgtg aagatcaggt ttttcgacag
     5881 attcacgtcc gtgaaaaact gacttgggaa gcggaacggg gagcgcggcg agttgccgat
     5941 ctggcccgac agcgtcgtct gaaacgccag cgggttgaag gcgtatggga tgcccccggc
     6001 cgggttgacg gggatgttct gagtcgggtc gctgatgagg ttcgggcggc tgccacgcgc
     6061 cgggacggtg gccgaaccct ggacgacgcg gtttaagggc agccccgtct gcgccgtcgt
     6121 gatgccggcg atttcccagc cgctcagcaa gaggcggccc gccgtcgtct tggcgaagcc
     6181 cgagccaaac gtcgggactt cgtagacgta gctggcgacg aagacgtgcg tgcggtcgag
     6241 acgcgagacg gcccgctcca gagtgaagtc ggtgcgaatc tgcggcacgt cgatggcgtc
     6301 gcggtcgtta gtcgcgtcgg tgatgttctt cgaccatgta tacgacatct gcgccgtcag
     6361 ccccttcgcc gtcctcatgc ggaacgagct gatgaggccg tggtagcgag aggtggcggt
     6421 cgtctgccgg tcggtgatcg tgccgaagcc gcggaaggga cgcgcgaggt tgatgcagtt
     6481 ggccgggttg ttcgaggcga ggagcgccgg gtcgcagccg gccacgccgc tcgcggcgtt
     6541 gagaatctcg gtcggggtcg gcgcgttgat gtccacgggc cggatcaggt ggttgccggc
     6601 ggagccggcg tagccgacct cgaagaccgt gttgcggaaa ggctcctgct gccaggtgag
     6661 cgaccactgc tggacgatgg gcgtgacgaa cgggtcggtc gtcgcgttga ccgtgccgag
     6721 gttgccgcgc gtcgtcgccg cgacgggcgc ggggctgccg aacgggaccg ggctggcgaa
     6781 cgtgcccgtc aggctgattg agttgttgaa aggcggcgtc gtgaaggaat tctgctcgac
     6841 gatgccgacg agcgcctgat cgtaatagac gccgtagccg ccgcgcagaa ccatcttgcc
     6901 ttcgccgcgc gggtcccagg cgaagccgat gcgcggcccc cagtcgttct tgtcgctctt
     6961 ctggacttgc cggccgaagg gcgaattctg gcccgcgtag gcgaagccgt tcgtcaggtc
     7021 gaaagtgctg agggtgaaca gggtgcaaga gacgttggcg cacgtcggcg cgcgcgaccg
     7081 gtcgtagaac tccggcagga acgtcgccag cacgtcttcc gtgtcatagg gctggcggaa
     7141 gcgatagtag cggatgccgt aatcgagttg gaagttgggg cgcactttcc acgtgtcctg
     7201 gaagtagaac tcggtgcgcc cgaagcgcag gtgctcgatc acgtcgcgct ccggctcggc
     7261 gtagctggcc gcgcgcccga gcaggaagtc ggccagcccg atgccggcgt tattgcccac
     7321 gcgcgtcgtg atgcccgtga agccgtaggt gccctgcgtg ttgctggccg cgttctcgtc
     7381 cttcgactcg aagctcacct ccccgccgaa cttgaacgtg tgcgacccgc ggatcatggt
     7441 tacgttgttc ttcgggttcc agttgcggta cttcacgtcg aagagctgcc cggagccgat
     7501 ggtgggctgc cccgtgatgt tgatgatcgg cggcaggccc gaattgttct gctcgaaaat
     7561 ctcgttgtgc gggacgctca agtccgagtt gtaacgcccg atgaggtctg acgtaatctt
     7621 gttaccggag aaggtgaagg tggcctcgtt gacgatgttc ggcccgaagg tgctggtcag
     7681 gccgatggct aacacctcgc cggggacgtt ggtgttggtc gttgctatgt cagggatcgt
     7741 ggcgccgaag aagaggccgc ccagctcgcg cgtcgcggag aggtcgtgcg tgtagcgacc
     7801 cgtgagccga tgggtcgagc tgaagttgtg gtcgatgcgg atagtttcct gccgtgtgtt
     7861 ctggatgttg ggcgtgacga cggcgtagcg gttcggcgcg cgccccgccg tggtcgaagg
     7921 gatgttcggc tcggggtaga ggctcaggag cgccaccgcg gccggattga ttcgcgccga
     7981 cgggatgatg ttgccgggga attgcaggtt cgtcagcggg tcgaagatcg gcaggttgcc
     8041 ggcctcggag aagttgccct gacgttcgag caggctgggc accgtgatgg ccgaagccag
     8101 actcggcgcg cggatgatgc ggcggaactc gtgcgagaac aggaagaacg tcttatcccg
     8161 attcgtgttg tagtggccgg ggatgaagat ggggccgctg acgagatagc cgaagttgtt
     8221 gtagcgcagc ttgggccgcg gcgtgcgcgg ctcgccggct gtggcaagcc ccgccagcac
     8281 agtcgcgtcg ttcggcccga aggtgccgtt ggcgttgttg aagaagctgt tggcgttgag
     8341 gcggtcgttg cggacgaatt cgtagagggt gccgtggaag tcgcttcccc cgccgcgcgt
     8401 gatgatgttg acgacgccgc cgccggagcg cccgaactcg gccgaaggca ccgaggtgat
     8461 gacgcggaac tcctgaatcg agtccaccgt cggcacggag agcagcgtga cgtttgagcc
     8521 aacgtcggtg ttggtcacgc cgtcaacgag gaagttgatc gagttgcgac gggtgccgtt
     8581 gatgctgaag tcaacgacgt tcgtcagtcc gatgccgatc tcggaggagc cggaactggt
     8641 gacgcccggg atgagggtga gcaactgcat gaagttgcgg ttgttgagcg gaatttcgac
     8701 gacgcgccgg ttctccacca cgtcgccgac cgtgggcgtc gtttggatga gaggcgcttc
     8761 gctcgtgacc gtgattgtct cgctgacggc gccggcttcg agcgtgaaat tgagcgtctt
     8821 ccggtcgttg acgttcactt caaacccctg actgacgtgc tccttaaagc cctgcgtgcg
     8881 gacggcgatg tcgtagacgc ccggccgaag ctgcgtgaac gtgtagttgc cgtcttcgtc
     8941 cgtctgtatc ttgcgctcct cgcgcgtgtt cgtactggtc agagtaacct cggcgttggg
     9001 cacggccgca cccgccgtgt cggtcacggt gcccagaatc gagccggtga cttcctgtgc
     9061 aaacgccgtc gtcgcgctca gtgttaaggc gcaggcgacg agggcgataa ttcgcttgaa
     9121 cattagctac ctccaaagag ttacagggga gtactgtcaa tgatgtctgt acaaatggta
     9181 aaggccggga gttgccgtcc ggtctcgcgc catccgtcgg atttttttcg tgtgcccttc
     9241 ctctactgat ctttacatct ttccacgcgg cgcgtgccgg attgcggcgc cggggcgcgc
     9301 ggccgtcagt tggccgtcgg cgacgaccga ttcgccgttg acgaagacgt ggatgatgcc
     9361 ggccgcgtac tggtgcggct ggtcgaaggt ggcgaggtcg gccacggtct tctcgtcgaa
     9421 gacgacgagg tcggcggcca tcccttcgcg caccagcccg cggtcgaaga ggccgaaggt
     9481 ctgggccggc agcgaggtca tcttgcggac ggcgtcttcg agcgggatga cgccctgctc
     9541 gcggacgtag cgcccgagga cacgggcgtt gttgccgtag ccgcgcgggt gcggcacgcc
     9601 ttcgccgtgg cggcgcacgc ccgagtcgga ggcgatcatc gtgaacggct cgcgcatgat
     9661 gcggcgcacg tcttcctcgc ccatcgaatg gtagaccatc gaggcgccgc ccgcctcgta
     9721 catcgagagt atctgttcga tctgcgagcc gatgtccgca cggccgcgcg cctgcttcgt
     9781 gatctcggcg atgttcttcc cgttgagcgc cgggtcggcg cggtggctgg cgacgaacgc
     9841 gtaagagtag tccttgaagc cgctccgctt caagccgtcg cgcatctctt tgacgacgcg
     9901 ggcgcgtgtc ggcttgtcgg ccagacgctt cttcccctcc tcgcgcccgc cttcgagcac
     9961 ccatgagggg atacgcgagt cgagcgaggt ggacgaggcc gtgtaggcgt attgatcgac
    10021 cgtcacctga agaccgcgcg cgcgggcctc gcgcacgagg cccaaggtct cgcccgtgcg
    10081 gccccaccag cgcttgttgc tgatcttgaa gtgggagatt tccacgggga gtccggcctg
    10141 ctcgccgatg tggatgctct cgcggatggc gtcgagcacg cgctcgccct cgttgcgcat
    10201 gtgggtggcg tagacgccgc cgtggcgggc ggcgatgcgg gcgagggcca ccacttcgcc
    10261 ggtcttggcg taggtgccgg gaacatagat gaggccggtg gagaggccga cggcgccgtc
    10321 gcgcatcgcc tgctcaacca gaccctccat cttttgcaac tcctcggccg atggctcgcg
    10381 gttggccgtg cccatcacgg cggcgcgcac cgagccgtgg ccgacgagcg tggcgaggtt
    10441 gacggcgagc ggcttgctac ggacgccgtc gaggtactcg gagacgttga cggccgagcc
    10501 gccgcagttg ccggagacta gagtggtcac gcccatgcgg acgaagtttt ccgcctcggg
    10561 caggttgtag atgtcttcga cgtgcgcgtg aacgtcgatg aagccggggg cgagaatctg
    10621 tccgcgggcg tcgatgacgc gcgcggcctc ctcgggggcg atgcggccga cgcgggcgat
    10681 gcggccttca cggacgccca cgtcggcgcg gaaccacggg ttgccggcgc cgtcaacgac
    10741 gcgggcgttc gtgatgacga gatcgtagcg ggcttccgaa gcggcgcccg tcgcggcgcg
    10801 tagccgcgcg ggttgctgcg agaagaggag tgcggcgacg gcggccgcga gggccgcgcg
    10861 cctcaggtgt cttttcatca aatcaaaatt cgcggacggg gaaaaggggc gcgggc
//
LOCUS       DASVES010000382         4401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_132888, whole genome shotgun sequence.
ACCESSION   DASVES010000382 DASVES010000000
VERSION     DASVES010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132888"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF240_15570"
     CDS             <1..531
                     /locus_tag="VF240_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176684.1"
                     /translation="KLELTDNAVRAHLLTLERDGLVRQSGVQRGARKPHFAYELTAEA
                     ERLFPKAYDALLNQLITALKGRLPAGALEDVLREVGRSLAARHASGQTPVDLEGRLQN
                     ALEVLKALGGSPSVEREDGRLIIRSGGCPLSAAVVEHPEVCELAEALVSEIVGLPVRE
                     RCVRGESPKCCFELGD"
     gene            complement(560..1507)
                     /locus_tag="VF240_15575"
     CDS             complement(560..1507)
                     /locus_tag="VF240_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7176685.1"
                     /translation="MKILIAYDGSQSADTGIDGLQRAGLPTQNVEALVVSVAEVWLPP
                     PGDEVIDDTFPLQVSAGVKRARERAARIVGQAQALAERGGERVRRIFPQWTISLEAAH
                     GSPACELLNRAGEWRPQLIIVGSRGHTALGRFVLGSVSQKVLTEAQTSVRVSRRATGA
                     GAAADHVLVGVDGSTGAQAAVRAVAARGWSPGSEVRVVVAQDLIKAFPVSLLIPPAGE
                     FVDEVNETESTQAEEIAAEAVKELRAGLDDKGVTVSSVIDAGDPKQVLVRHAEEFGAD
                     CIFMGATGFSSRVERFLLGSVSAAVAARAHCSVEVIRAG"
     gene            complement(1606..2517)
                     /locus_tag="VF240_15580"
     CDS             complement(1606..2517)
                     /locus_tag="VF240_15580"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX7176686.1"
                     /translation="MLVNGLVTKAVSDEENRPARWRWDAFARFSEQLAASPWVHRLAR
                     LGYAAEGLLYVIVGGTASLAAVNVGGRVRGARGALNLIVAQPFGRLVVAFVAVGLCGY
                     ILRRFVQVLVEPAEGVPPKPIMRILRRVGFALSGLAHVGIALAALRLVLGLAALSSGG
                     PPAARGWATLLFVWKPLDGWLTLLVGLVIIGVAIFYFYKAVSRRFTMDLELERMSRRV
                     ERATLACGVAGYAGRGTGFLIMGAFLVYAGWYVEEVEARGFGDILRALEEQPFGAWIL
                     IAAAVGLTAYGVYLLLAARHLRLIAAW"
     gene            complement(2480..3571)
                     /locus_tag="VF240_15585"
     CDS             complement(2480..3571)
                     /locus_tag="VF240_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7176687.1"
                     /translation="MAFARRVLIATLVVVCVVLVLYFVWYAADLLMLVFAGVLVSILL
                     RGFSRFLGQKTGIGRGLSLAIVAVALVALIIAVALLVTGRIGEQMSELRQQLPQAVEN
                     VKRYFGQYEWARNAFDGLPNLNDWLAERGGTIVTRLTGLASTTLGTVVNVLVVVVIGL
                     YLASQPDLYSRGIKHLLPFRHRERAGEILGVIDAALWHWLGGRFGLMLVNGGLTALGL
                     WLLGVPLALTLGLLAGLLNFVPNFGPWIAAVPAVLIAFLQGPQQALYVALLYLALQSV
                     DGYILTPLVDRKSVELPPVLTITAQVLLGLAFGFIGILLASPLTAVAMILVKMLYVED
                     VLGDHVMHEGGEAEGEKGAGKRAGDEGRV"
     gene            complement(3743..3910)
                     /locus_tag="VF240_15590"
     CDS             complement(3743..3910)
                     /locus_tag="VF240_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176688.1"
                     /translation="MKVWATLDPTDGGLAINNKDTSASGVVQLAVPGFSSGIVKRMTT
                     PSANHEDQEMS"
     gene            complement(4045..>4401)
                     /locus_tag="VF240_15595"
     CDS             complement(4045..>4401)
                     /locus_tag="VF240_15595"
                     /inference="COORDINATES: protein motif:HMM:NF022766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HEX7176689.1"
                     /translation="DKSRVINLSATLSIRNTDAQNPISIASVRYYGTNGALVREYLNK
                     AISLAPMASTDFVVAEDDTSGGAGANFIVEWSAGAEVTEPVVEAVMISTAQQQGISFV
                     SAGRVIKDRTPRASGR"
BASE COUNT          882 a         1497 c         1279 g          743 t
ORIGIN      
        1 aagctcgaac tgacggacaa cgccgtccgc gctcacctgc tcacgcttga acgcgacggc
       61 ctcgtccggc agagcggcgt gcagcgcgga gcgcgcaagc cgcatttcgc ctacgagctg
      121 acggccgagg ccgagcgcct cttccccaaa gcctacgacg ccctgctcaa ccaactgatc
      181 accgcgctga agggccgcct cccggcgggc gcgctcgaag acgttctgcg cgaggtcggc
      241 aggtctctgg ctgcccggca tgcttcaggc cagacacccg ttgacctgga gggccgattg
      301 cagaacgcgt tggaggtgtt aaaagctctc ggcgggtcgc cgagtgtgga aagagaggac
      361 ggcaggctaa tcatccgcag cggcgggtgc ccgctctcgg cggcggtcgt cgagcacccg
      421 gaggtgtgcg agctggcgga agcactcgtc tccgagatcg tcggcctgcc ggttcgggag
      481 cgctgcgtcc ggggggagtc ccccaagtgc tgctttgagc tgggcgacta gaatgaagca
      541 ggtgccttgg atttgcaggc taacctgcgc ggatcacttc gaccgagcag tgcgcgcgcg
      601 ccgcgactgc cgccgacacg ctccccaata agaagcgctc gacgcggctg ctgaagccgg
      661 tcgcgcccat aaagatgcag tcggcgccga actcttcggc gtggcggacg agcacctgct
      721 tcgggtcgcc tgcgtcaatc acagacgaca cggtgacgcc tttgtcgtcc agcccggcgc
      781 gcagttcctt caccgcctcc gccgcgatct cctccgcctg cgtgctctcc gtctcgttca
      841 cctcatccac aaactcgccc gccggtggga tgaggaggga gacagggaag gctttaatca
      901 aatcctgcgc gacgacgaca cgcacctcac tgcccggcga ccaaccgcgc gcggcgaccg
      961 cccgcacagc cgcctgcgcc cctgttgaac cgtcaacgcc gacaaggacg tgatcggccg
     1021 ccgctcctgc gcctgtcgca cgacgcgaga cgcgcactga agtctgcgct tcggtcaaca
     1081 ctttctgtga cacgctgccc agcacgaacc ttcccagcgc cgtgtgaccg cgcgaaccga
     1141 cgataatgag ttggggccgc cattccccgg cgcgattcag caactcgcac gcgggcgagc
     1201 cgtgagcggc ttccaggctg atcgtccact gcggaaagat gcgcctcacg cgctcgcccc
     1261 cgcgctccgc caacgcctgc gcctgtccga cgatgcgcgc cgcgcgttcg cgcgcacgtt
     1321 tgacgcccgc cgaaacctgg agcgggaagg tgtcgtcgat cacctcgtcg cccggcggcg
     1381 gaagccacac ttccgcgacc gagaccacga gcgcctcgac gttctgcgtt ggcagccccg
     1441 cgcgctgcaa cccgtcaatc cctgtgtcgg cgctctgaga gccgtcgtaa gcaattagta
     1501 ttttcatcct tgaattcacc tcctgagaat atccggcgcg agaatgatat atcttccgac
     1561 tgcggcaccc gtgattgaat caaccataaa tggccgtaag agtaattacc aggcggcgat
     1621 tagccgcaga tgccgcgccg ccagcagcag gtacaccccg taggcggtca gccccacggc
     1681 ggcggcgatc aaaatccacg ccccgaaggg ctgctcttcg agcgcgcgga ggatgtcgcc
     1741 gaacccgcgc gcttcaactt cctccacgta ccacccggcg tagacgagaa acgcccccat
     1801 aatcagaaac cctgtgccgc gccccgcata gcccgcgacg ccacaggcta acgtcgccct
     1861 ttcaacgcgg cggctcatgc gttcgagttc gaggtccatc gtgaaccggc ggctcaccgc
     1921 cttgtagaaa tagaatatgg cgacgccgat aatgacgagc ccgactaata gggtcagcca
     1981 cccgtcgagc ggcttccaca cgaacaacag ggtcgcccat ccgcgcgcgg ccggcggccc
     2041 gccggaactc agggccgcca gccccagcac cagtctgagc gccgccagcg cgatgccgac
     2101 gtgcgcgagc ccgctcaagg caaaacccac gcgtctgagg atacgcatga tcggctttgg
     2161 cggcacgcct tcggcgggct cgactaagac ctgcacgaag cggcggagga tgtagccgca
     2221 gagtccgacg gcgacgaagg cgacgactag ccgcccgaac ggttgcgcga cgatcaggtt
     2281 gagcgcgccc cgcgcccccc tcacgcgccc gccgacattg actgccgcca gcgacgccgt
     2341 gccgccgacg atcacgtaaa gcaaaccttc cgccgcgtag ccgagcctcg ccagccgatg
     2401 gacccacggg ctggccgcga gttgttcgga gaatcgggca aaggcgtccc atcgccaccg
     2461 cgccggtcgg ttctcctcgt cagacacggc cttcgtcacc agcccgttta ccagcacctt
     2521 tctcaccctc cgcctcgccg ccctcgtgca ttacgtggtc gccgagcacg tcttcgacgt
     2581 agagcatctt gacgagaatc atggcgaccg ccgtgagcgg cgatgcgagt aggatgccga
     2641 tgaagccgaa cgccaacccc aacagcactt gcgccgtgat cgtcaacacg ggcggcagtt
     2701 ccacgctctt gcggtcaacg aggggggtca gaatgtagcc gtcaactgat tgcagcgcga
     2761 gatagagcag cgcgacgtac agggcttgct gcggaccttg taagaaagcg atgagcacgg
     2821 caggtacggc cgcgatccac ggtccgaagt tcgggacgaa gttaagcagc cccgcgagca
     2881 gaccgagcgt cagcgcgagc ggcacaccca agagccacaa ccccagagcc gtcaacccgc
     2941 cgttgacgag catcaagccg aagcgaccgc cgagccagtg ccacaacgcc gcgtcaatga
     3001 cacctaagat ttctccggcg cgctctcgat gccggaaagg cagcaggtgt ttgatgcctc
     3061 ttgaataaag atcaggctgc gaagcgaggt agagaccgat gacgacgacg acgaggacgt
     3121 tcacaaccgt gccaagtgtc gtcgaagcca accccgtgag gcgcgtgacg atggtgccgc
     3181 cgcgctcggc gagccaatcg ttgaggttcg gcaaaccatc aaacgcgttt cgcgcccatt
     3241 cgtattgtcc gaagtagcgt ttcacgttct cgacggcttg cggaagttgc tgtcgcagtt
     3301 cgctcatctg ctcgccgatg cgacccgtca ccaacaacgc tacggcaatg atgagggcga
     3361 cgagcgcgac cgcgacgatt gccagcgaaa gcccgcgccc aatgcctgtt ttctgaccta
     3421 aaaacctgct gaagccgcgc agcagaatcg agacgagcac gcccgcgaag acgagcatca
     3481 gcaaatccgc cgcgtaccaa acgaaataga gtacgagcac cacgcacacc acgacgaggg
     3541 tcgcgatgag cacgcgccgc gcgaacgcca acgactcgct ttcccgtccc gcctctgtct
     3601 ctccccgcga gttcaaatct gaagtgattt tcatttgttt caccagcggt catcactcaa
     3661 ttcaacagcg gtagatacaa atggatttgc gattttatta attttcgaca cctaccgaat
     3721 ttgacgactc ccgggcttta cctcaagaca tttcctggtc ttcatgattg gccgacggcg
     3781 tggtcatgcg ttttacgata ccggaactga agccggggac agctaactgc accacgccgg
     3841 atgccgacgt atctttgtta ttgatcgcca gaccaccgtc ggtcgggtcg agcgtcgccc
     3901 acactttcac aggcgcgccg aagacctcgg ggttacaaaa cacatcacca gaatttctct
     3961 ctgaattaca gatatggtca caaagcgggt agtgacttaa acagatcttc cgccgatgga
     4021 cgaccgggct tttctaacct cgcatcacct gcccgatgcc cggggtgttc gatccttaat
     4081 gacgcgccct gcactcacga atgaaatccc ttgctgctga gccgtgctga tcatgaccgc
     4141 ctcgaccacg ggctccgtca cctcggcgcc ggcgctccac tccacaatga agttagctcc
     4201 cgccccgccg ctcgtgtcgt cctccgccac cacaaagtcg gtcgaagcca tcggagccag
     4261 actgatcgcc ttgttcaggt actccctaac cagcgcacca ttcgtgccgt agtagcgcac
     4321 cgaggcgatg cttataggat tttgagcatc cgtgttccgg atgctgagcg tggcgcttaa
     4381 gttgatcacg cggcttttat c
//
LOCUS       DASVES010000383         5844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1005495, whole genome shotgun sequence.
ACCESSION   DASVES010000383 DASVES010000000
VERSION     DASVES010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005495"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1889
                     /gene="dnaG"
                     /locus_tag="VF240_15600"
     CDS             42..1889
                     /gene="dnaG"
                     /locus_tag="VF240_15600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7176690.1"
                     /translation="MRFPQTFIDDLRRQADIVRVVGDYVTLKKKGTNWMACCPFHQEK
                     SPSFSVSPSKDIFYCFGCSKGGSVFNFVMEMERVSFPEAVRIVAEKSGMPLPKMEGEG
                     KFEARRQEADAVVELNAWALEWWEQQFGGDNEEARFAREYVEGRGITEETRRAFRLGY
                     APNSWDALSSHLRRKGASAADIEGSGLVVKKDAGGSYDRFRGRVIFPVLDAQGRAVAF
                     GARAIRAGDEPKYLNSPETAAYTKGRHLFGLNLTRDEIRLKKFAILVEGYLDLIVPYQ
                     LGVKNVVASLGTALTPEQARLLARFARRVVVNYDGDRAGVSAAKRAIETLLAEDFEVK
                     VLALPDGADPDEFLRAHGAEEYNRRRGEALPHIQFVLAQAQRDRNLRSPAEKAQAVDE
                     VLPYLRAVRHPVQKREYFDMAMDALRVDDDALRNDLWKTISPRAQAAVASSAARPAPE
                     EVKERFARAAALRPTVAEHRLLELLVHDAELRRDVLPRLEEDDYAELPTAAVFRALRE
                     LHARGADPDFTALSELTAGDDVASDFVPLLLMGESERAEGEATDDLLAEAESCLQALR
                     LMRVDWRIRELAAEITEADRAGDEARRDRLIMEDLELKRRKQQMSEVRN"
     gene            1974..3704
                     /gene="rpoD"
                     /locus_tag="VF240_15605"
     CDS             1974..3704
                     /gene="rpoD"
                     /locus_tag="VF240_15605"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7176691.1"
                     /translation="MEEKDQDDVMQRLLELGGDKKFLTFDDLNRELPDNIVSPDDIED
                     VLQKLEGSGVPVADSDERLIEQATVVVSPEDLDGELDEDIELDLSAGALEKTNDPVRL
                     YLREMGVVPLLTREGEVSIAKRIERGQVKTQKAISRSPIAVRELLRMGDELEAGKTAI
                     RDVVTFSDQAELTGQEDKADEYLQWTVEGINNIRRVFKAGLKEWERLRAETKLTRGKK
                     SKKMLRLRRKVARMRLDISQEMKGLNMTEKARQRLIDSIRKVHREVRVQERDTETYNE
                     KLTRKRIRPDEEREWKRQITNARRRLQQIEAEHGVSPIEIKRSLQSIVTGEHETAQAK
                     RELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEWRRGYKFSTYATWW
                     IRQAITRAIADQARTIRIPVHMIETINKLIRTSRALVQELGREPTSDEIARKMDIPVS
                     KVRKVLKIAQEPISLETPIGEEEDSHLGDFIEDKSIQNPADSVVASNLREITDEVLAT
                     LTPREEKVIKMRFGLGSSGSEHTLEEVGQHFAVTRERIRQIEAKALRKLRHPSRSRKL
                     KAFLEGTRPV"
     gene            3796..5670
                     /locus_tag="VF240_15610"
     CDS             3796..5670
                     /locus_tag="VF240_15610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7176692.1"
                     /translation="MSKKIIESYRRRLAREEGHITKRGARLRVALCYPNVYAVGMANL
                     GFHSVYEIFNQIPEVSCERVFLPDDDEMREYERAGTSLLSLETQTSVGDFDVLAFSIS
                     FETDYYHMAQLLRLARIPLLAAERTHHDPLIVMGGAASFLNPEPIADFTDVVAVGEGE
                     ILVPRLADIILENADEGGTKEDLLLKLARNGRGFYVPSLYEVTHNDDGTIAAYEVTAE
                     GVPAKVGRMVAAENPKEGSLRRAIRRGDWDVAARLKGEEVFAPSTVVWAPDAEMGERF
                     LIEISRGCSQGCRFCWAGFNYWPPRVVPARDILAKAAAWRSKTDKIGLVSTAVCDHPE
                     ISEILSGLRAMDYKISVSSLRLDQIEDELLDALVDSGDHQIAVAPETGSDRLRKVINK
                     NLTNDEIVDICGAVFDRGMLNLKLYMMAGLPTETEEDLEAMVALVERVKDRMIETGRR
                     FGRVGKIIPALNGFVPKPNTPFQWEPICEERELKRRLKWLSKNLARIPNVEVRVMSAR
                     VAHEQALFSLGDRSVGRVIESMTRHRGDLQAALRETNFDADFHTGRRRTYDEVLPWEI
                     VDAGLGREFMEREHERAQIAKSTAPCPSVDTCTRCGVCPTTWLAAAPPGLVQLTAAAV
                     "
BASE COUNT         1124 a         1934 c         1880 g          906 t
ORIGIN      
        1 cccggtagca tgatgtagac cctttcgccg ccacgaaaac gatgcgcttc ccgcagacat
       61 tcatcgacga cctgagaagg caggcggaca tcgtccgcgt ggtgggcgac tacgtgacgc
      121 tcaagaagaa gggcacgaac tggatggcct gctgcccgtt ccatcaggag aagtcgccct
      181 cgttctcggt cagcccgtcg aaagacatct tctattgttt cgggtgtagc aagggaggtt
      241 cagtcttcaa cttcgtgatg gagatggagc gcgtgtcgtt ccccgaggcg gtgcggatcg
      301 tggcggagaa gtcggggatg ccgctgccga agatggaggg cgaggggaag tttgaggcgc
      361 ggcggcagga ggccgatgcg gtcgtcgagt tgaacgcgtg ggcgctggag tggtgggagc
      421 agcagttcgg cggggacaac gaggaggcgc gcttcgcgcg tgagtacgtc gaagggcgcg
      481 gcatcacgga ggagacgcgg cgggcgtttc ggctcggcta cgcgccgaac agttgggacg
      541 cgctgagttc gcacctccgg cgcaagggtg caagcgcggc cgacatcgag ggcagcggcc
      601 tcgtcgtcaa gaaggacgcg ggcggctcct acgaccgctt ccgcgggcgc gtcatctttc
      661 ccgtgctcga cgcgcagggg cgagccgtcg ccttcggcgc acgcgccatc cgcgccggcg
      721 acgagccgaa gtatctcaac tcgcccgaga ccgcggccta caccaagggc cgccacctct
      781 tcggcctcaa cctcacgcgc gacgagatca ggctcaagaa attcgccatc ctcgtcgagg
      841 gttatctgga cttaatcgtc ccctatcagc tcggcgtgaa gaacgtcgtg gcgagtctcg
      901 gcacggccct gacgccggag caggcgcgtc tgctcgcgcg gtttgcgcgc cgcgtggtgg
      961 tcaactatga tggcgaccgc gccggagtct cggccgcgaa acgggccatc gaaacccttc
     1021 tcgcggaaga cttcgaggtc aaggtgctgg ccctgcctga cggggccgac ccggacgaat
     1081 tcctgagggc gcacggcgcc gaagagtaca accggcggcg gggggaggcg ctcccacaca
     1141 tccaattcgt gctcgcgcag gctcagcgcg accgcaacct ccgcagcccg gccgagaagg
     1201 ctcaggccgt ggacgaagtg ctcccatacc tgcgcgccgt ccgtcatccc gtccagaagc
     1261 gcgaatactt cgacatggcg atggacgcgc tgcgcgttga cgacgacgcc ctgcgtaacg
     1321 atctttggaa gaccatcagc ccgcgtgctc aggcggccgt ggcatcttcg gccgcgcggc
     1381 ccgcgcccga ggaggtcaaa gagaggttcg cgcgcgccgc cgcgctccgg ccgacggtcg
     1441 ccgagcaccg tttgcttgag ctgctcgtcc acgacgcgga gttgcgccgc gacgtgctcc
     1501 cgcgactcga agaggacgac tacgcggagc tgcccacggc cgccgttttc cgcgcgctca
     1561 gagagctgca cgcccgcggc gccgaccccg acttcaccgc cctgagcgag ctgaccgccg
     1621 gcgacgacgt ggcctcggac ttcgtgccgc tgctgttgat gggcgagtcg gagcgcgccg
     1681 agggcgaggc gaccgacgac cttctggccg aggccgagag ctgtctgcaa gccctgcgcc
     1741 tgatgcgcgt tgactggcgt atccgagaac tcgccgccga gattaccgag gccgaccgcg
     1801 ccggcgacga ggcgcgccgc gaccgcctca tcatggaaga cctggagttg aaacgccgca
     1861 aacaacagat gtcagaggtc aggaattagg cggggccgaa aacttgcgtt accggcctcc
     1921 agcctctaat ctctgacctc tcgtttttcg gaggtggaca acaagccttg agcatggaag
     1981 agaaagatca ggacgacgtc atgcagcgcc ttctggagct gggcggcgac aaaaagtttc
     2041 tcaccttcga cgacctcaac cgcgaactcc ccgacaacat cgtctccccg gacgacatcg
     2101 aggacgtgct ccagaagctc gaaggctccg gcgtccccgt cgccgattcc gacgagcgcc
     2161 ttatcgagca ggccacggtc gtcgtctccc ccgaagactt ggacggcgag cttgacgagg
     2221 acatcgagct tgacctctcc gccggcgcgc tggaaaaaac caacgacccc gtccgcctct
     2281 atctccgcga gatgggcgtc gtcccgctct taacgcgcga gggcgaagtc tccatcgcca
     2341 agcgtatcga gcgcgggcag gtcaagacgc agaaggccat ctcgcgctcg cccatcgccg
     2401 tgcgcgaact cctccgcatg ggcgatgagc tggaagcggg caagaccgcc atccgcgacg
     2461 tggtcacctt ctccgatcag gctgagctga cggggcagga ggacaaggcc gacgagtatt
     2521 tgcagtggac ggtcgagggc atcaacaaca tccggcgcgt cttcaaggcg ggcctcaagg
     2581 agtgggagcg gctgcgcgcc gagacgaagc tgacgcgcgg caagaagtcg aagaagatgc
     2641 tccggctccg gcgcaaggtg gcgcgcatgc gcctcgacat ctcgcaggag atgaaggggc
     2701 tgaacatgac ggagaaggcc cgccagcgcc tcatcgactc catccgcaaa gtccacaggg
     2761 aggttcgcgt ccaggagcgc gacaccgaga cttacaacga gaaactgacg cgcaagcgca
     2821 tccgccccga cgaagagcgc gagtggaagc gtcagatcac gaacgcccgc cgccgcctcc
     2881 agcagatcga ggccgagcac ggcgtctccc ctatcgagat caagcgttcg ctccagtcaa
     2941 tcgtcaccgg cgagcacgag accgcacagg ccaagcgcga actcatcgag gccaacctcc
     3001 gcctcgtcgt ctccatcgcc aagaagtaca ccaaccgcgg cctccagttt ttagacttga
     3061 ttcaggaggg caacatcggc ctgatgaagg ccgtggacaa gttcgagtgg cggcgcggct
     3121 acaagttctc gacctacgcg acgtggtgga ttcggcaggc catcacccgc gccatcgccg
     3181 atcaggcgcg caccatccgc atccccgtcc acatgatcga gaccatcaac aagctcatcc
     3241 gcaccagccg cgcgctcgtg caggagctgg ggcgcgagcc gacgagcgac gagatcgcgc
     3301 gcaagatgga catccccgtc tcgaaggtgc gcaaggttct gaagatcgcg caggagccga
     3361 taagtctgga gacgcccatc ggcgaggagg aagactcgca cctcggcgac ttcatcgagg
     3421 acaagtcgat ccagaacccg gccgactcgg tcgtcgcctc caacctgcgc gagattacgg
     3481 acgaggtgct ggcaacactg acgccccgcg aggagaaggt catcaagatg cgcttcggcc
     3541 tcggctcatc gggttccgag cacacgctcg aagaggtcgg ccagcacttc gccgtcaccc
     3601 gcgagcgcat ccgccagatt gaggccaaag ccctgcgaaa gctccgccac ccctcgcgct
     3661 cgcgcaagct caaagccttt ctcgaaggca cgcggccggt ctgacagcgg gcgcggcgga
     3721 cgtttaactg gtaaactgat ggcaagcctg aagcatcaaa gctcgggctt gccatcaacg
     3781 ttttaagtcg aaagtatgtc gaagaagatc atcgaaagtt accggcgcag gcttgcgcgc
     3841 gaagaggggc acatcacgaa gcgcggcgcg cgcctgcgcg tggcgttgtg ctacccgaac
     3901 gtctacgccg tcggcatggc caacctcggc ttccactccg tctacgaaat cttcaaccag
     3961 attcccgagg tctcctgcga gcgcgtcttt ctgcccgacg acgacgagat gcgcgagtac
     4021 gagcgcgcgg gcacgtcgct cctgagtctg gagacgcaga cgagcgtcgg cgacttcgac
     4081 gtgctggcct tctccatctc gttcgagacc gactattacc acatggcgca actgctgcgc
     4141 ctcgcgcgca tcccgctgct cgccgcggag cgcacgcacc acgacccgct catcgtcatg
     4201 ggcggcgccg cttcgtttct caaccccgaa cccatcgccg acttcacgga cgtggtcgcc
     4261 gtcggcgagg gcgaaatcct cgtcccccga ctcgccgaca tcatccttga gaacgccgac
     4321 gagggcggca cgaaggaaga cctgctgctg aagctggcgc gcaacggtcg gggcttttac
     4381 gtcccctcgc tctacgaggt gactcataac gacgacggca ccatcgccgc ctacgaggtg
     4441 acggccgagg gcgtgcccgc gaaggtcggc cgcatggtcg cggccgagaa cccgaaggaa
     4501 ggctctctgc ggcgcgcgat tcgccgcggc gattgggacg tggccgcccg cctcaagggc
     4561 gaagaggtct tcgcaccctc cacggtcgtc tgggcgccgg acgcggagat gggcgagcgc
     4621 ttcctcatcg agatttcgcg cggatgctcg caggggtgcc gcttctgctg ggccggtttc
     4681 aactactggc cgccgcgcgt cgtcccggcc cgcgacatcc tcgccaaagc cgccgcgtgg
     4741 cgctcgaaga ccgacaagat cggcctcgtc tccacggccg tctgcgacca ccccgagatc
     4801 agcgagattc tctcgggcct gcgcgcgatg gactacaaga tttcggtctc cagcttgcgg
     4861 ctcgaccaga tcgaagatga actcctcgac gcgcttgtcg attccggcga ccaccagatc
     4921 gccgtcgcgc ccgaaacggg gagcgaccgc ctccgcaaag tcatcaacaa gaacctgacg
     4981 aacgatgaga tcgtggacat ctgcggggcg gtgttcgacc gcgggatgct caacctgaag
     5041 ctctacatga tggcggggct gccgaccgag accgaggaag acctcgaagc gatggtcgcg
     5101 ctcgtcgagc gcgtcaaaga caggatgatc gagacgggcc ggcggttcgg ccgcgtcggc
     5161 aaaatcatcc cggccttaaa cggcttcgtg cccaagccga acacgccctt ccagtgggag
     5221 ccgatctgcg aggagcgcga gctgaagcgg cgtctgaagt ggctctcgaa gaatctggcg
     5281 cgcatcccca acgtcgaggt gcgcgtgatg tcggcgcgag tggcgcacga gcaggcgctt
     5341 ttttcgctcg gcgaccgctc ggtcggccgc gtcatcgagt ccatgacgcg ccaccgcggc
     5401 gaccttcagg ccgccctgcg tgaaacgaac ttcgacgccg acttccacac cggccgccgg
     5461 cgcacctacg acgaggtgct gccctgggag atcgtggacg ccggcctcgg tcgcgagttc
     5521 atggagcgcg agcacgagcg cgcgcagatc gccaaatcga cggcgccctg cccctcggtg
     5581 gacacctgca cgcgctgcgg cgtctgcccc accacctggc tcgccgccgc gccgcccgga
     5641 ctcgtccagc tcaccgccgc cgcggtctga agtaaggcaa aagtaaatgg aaaagcgtta
     5701 ccaagtgttc gtaagctcca cttacgctga cctacgcgag gaacgccagc gggtgattca
     5761 agccctgatg gagatggact gtatcccttc cggcatggaa ctattccccg ctgctgatga
     5821 ggaacaatgg gagtttatca atag
//
LOCUS       DASVES010000384        10452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1481346, whole genome shotgun sequence.
ACCESSION   DASVES010000384 DASVES010000000
VERSION     DASVES010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481346"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VF240_15615"
     CDS             complement(<1..695)
                     /locus_tag="VF240_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176693.1"
                     /translation="MKRFKQGRSTASVLVVALALLCASLGPLSAPTALAQSRRVPAQE
                     PQKKNKRPDPGQQPRPGEQPPDVEPSPPADAIEDTEPVKVSTALVNLEAVVIHKKTKQ
                     VITGLKKENFAVFEDGVQQQVSNFGTPEAPITVAMVLEYSNLMDQKLRADNYEPGLFE
                     VLRPMAMFLSQFIKPPADYVSVIAYDMRVTPITDFTNDPTRINQTINLLLRNRPVSSE
                     ANLFDALKLTLVGG"
     gene            complement(947..1783)
                     /locus_tag="VF240_15620"
     CDS             complement(947..1783)
                     /locus_tag="VF240_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176694.1"
                     /translation="MKVKFSRAIVAVALAVAASSAVLADTIRLKNGSIIRGQVVGFRD
                     EQFTVLMEPTARGRRNRIQVYIEEVESIEFDGAPAGSTTADAGDTQTAAPVDNRASNP
                     APSRNTSQPSEQPRMQGAGNPPPANNSRTSDTGAAQPSPSSSSPSSQFFPVRVRVRAD
                     NAANGWTDSGLMVRKGQRLRISASGRISLGQGRFATPTGLSRVADNEKLMRDEPTGAL
                     IAVVGDDNDEFVYIGAGREFYATRDGRLFLGINEGNLADNTGTYDALIEAEPVTSGQA
                     RP"
     gene            2118..2792
                     /locus_tag="VF240_15625"
     CDS             2118..2792
                     /locus_tag="VF240_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176695.1"
                     /translation="MEFAACTPRPVSPDGFNPEAVLPFGLTAAHVHHAMDDFIRLMHV
                     ANVQLHGKQIQRLETILAPANFSGMVGDFMAATIPNYCDGLARNMFHNGHPDMIPKGR
                     FPDDSVQYAHEGIEIKASRYLAGWQGHNPENAWLMVFVFGSNRPADALKGMAPRPFEF
                     QMVVGAQITEADWGVAGRREGGRRTPTAGVLRTGREKMMANWIYKAPDLTRKEEATLL
                     AAPPPS"
     gene            complement(2743..3711)
                     /locus_tag="VF240_15630"
     CDS             complement(2743..3711)
                     /locus_tag="VF240_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX7176696.1"
                     /translation="MTTAKLVEAPTGLALDYTHRVIGRSLILHADCLEWLSRIPENSL
                     HGIVTDPPYGVKEYDFDQIEKRANGNGGIWRIPPSFDGHTRQPLPRFTALNQKERKQI
                     HTFFVEWAKVVERSLRPGAHLFIATNAFLSQLVFSALVAGGLEFRGELIRFVRTFRGG
                     DRPKNAENEFPNVSSLPRSAYEPWGILRKPLTKGMKVSECLREFQTGGLRRTPDDKPF
                     TDIIPSGRTPRSEKEIAGHPTLKPQAFMRQIVHAVLPLGVGVIADPFMGGGSTIAAAE
                     AMGVASVGVERFVDYYELSIKAIPKLAAIQTTREEEQQEGSLLPFE"
     gene            complement(3708..3899)
                     /locus_tag="VF240_15635"
     CDS             complement(3708..3899)
                     /locus_tag="VF240_15635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7176697.1"
                     /translation="MNLLTTKEVAERLGVTPRRVLAMIQAEQLPAEKFGRDYMIKEKD
                     LKLVEDRKVGRPRKNGMHH"
     gene            complement(4062..4138)
                     /locus_tag="VF240_15640"
     tRNA            complement(4062..4138)
                     /locus_tag="VF240_15640"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4102..4104),aa:Ala,seq:cgc)
     gene            complement(4385..5608)
                     /locus_tag="VF240_15645"
     CDS             complement(4385..5608)
                     /locus_tag="VF240_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX7176698.1"
                     /translation="MSTERLYYADSHLLEFDARVITSYKVEGGRRELVLDRTAFYPTG
                     GGQPFDTGAIGDARVVDCLEGDDGKVVHVVEGDAPGVGETVHGRVDWPRRLDHIQQHT
                     GQHLLSQALVELHGAQTRSFRMTADACEIDVELRDPSDERVWRAVERANEVVWGNRPV
                     SVHHVTAEEAARLPLRKDSAREGELRVVEIEGFDFSPCGGTHAQRTGEVGTIVARGWE
                     RAKGMTRIEFAAGVRAARDYARANQAARAASSLLTVGREEIGRSVERLLEENKQLARR
                     NRALEDVAARVEADELLAESAARDAAGASIVARVFDARDAESLRRIASALVSRPAAPS
                     VVALLGARDGDAARLVFARTASAPGDMGALMRDACATLEGRGGGRPDMAQGGGRRFDL
                     LADVIDAAARSLEAT"
     gene            5769..7238
                     /locus_tag="VF240_15650"
     CDS             5769..7238
                     /locus_tag="VF240_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX7176699.1"
                     /translation="MTDARDVDLRELARREADEAGFATGFGPEVESELRELGRGGRGV
                     EGSEGVRDLRELLWSSIDNRESRDLDQVEYAERLPGGEVRLRIGVADVDSCVPRGSAI
                     DRHARANTVSVYTPGAVFHMLPEQLATELTSLLEDAERLAVVTELTVAEDGRVIATDV
                     YRAAVRNRRKLVYEPVGEWLDGRSGVPAEVAGVEGLEEQIRLQDETAERLRALRRTNG
                     ALELETVEADFVVVDGRAVDLVTKTRTRSQSIIENFMVAANTSMAEYLEARGVPSLRR
                     VVRRPERWSRMAEIAERFNERLPREPDSRALSDFLARRRRADPAGYAELSLSVMKLVG
                     SGEYLVEAPGREQEGHFGLAVDDYTHSTAPNRRYPDLVTQRCLKATLAGGASPYTVSE
                     LGEIAEHCNRMEDAARKVERRMRKAATAMLLGARAGEVFEAVVTGAKPKGTFVKLAAP
                     PAEGRVVRGERGLDVGDRVRVRLVSTDPERGFIDFARVS"
     gene            7423..7902
                     /locus_tag="VF240_15655"
     CDS             7423..7902
                     /locus_tag="VF240_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176700.1"
                     /translation="MSNDPKEMSDEEIRAQVVELGFGGDRRRFEEFCRVLREGLPVGT
                     GVALRGSVITNERYEDGRPFDAKGKGTSDLDVTLVGEEVRECWKQDAYYIPGLHTMPL
                     HDEDPDCAPNLDGLRRELQQIARRPVSFQATSNIVLFARDVLMSQPYFMLIEQAEAP"
     gene            7899..8657
                     /locus_tag="VF240_15660"
     CDS             7899..8657
                     /locus_tag="VF240_15660"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7176701.1"
                     /translation="MILKLLSYNIRFGGRGRERALADVIRAAAPDLVVFQEATDTRVV
                     EAVAESAGLPHHAARRDHSIAYAGRIEVAHHAWHHPAGSRHPFMELVLADDHTRVFGL
                     HLSARFSKWSERRRAREMRALLDGIREHQEGFHVLVGDFNTLAPGELLELSRMPRWIR
                     ALVWLSGRELQRDTIQMLLDAGYRDGFRLLHPDVKGYTLPAWDPSVRLDYVFLPARDA
                     ARLKSCDVLDAAGAAAASDHLPLLAEIDLPVAAA"
     gene            8753..>10452
                     /locus_tag="VF240_15665"
     CDS             8753..>10452
                     /locus_tag="VF240_15665"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176702.1"
                     /translation="MFVRVRLSLRRAVVASAFVLVQLISSPDALAQQGTNAGPPLRIS
                     YTLSMPAPETHLFEVQVDVAGLRGNTPLEFQMPRWSPGRYAIFDFAKNVQEARAVTLC
                     PPGAQCKGRELAVRRLDTQTWSVETADGGASFRYKVFADDLSGTFSQLDTRHASFNGG
                     SVFVYVGGHKQDPVTLKIRAPAGWKVANGYTRSPEQTEFAFPNYDVLIDTPTDVAPDW
                     TMDTFDVDGKTYRVLVHSFGPEGGKRAAFVRDIEKVVREQVRMMEGAPDYEQYTFLFH
                     FDPTARGGDGMEHLNSTQIVSTRALADEGAYAGSLSTAAHEFFHVWNVKRLRPAGLGP
                     WDFTRPVVTRGLWIAEGFTNYYETLSRRRAGVIDDAHLLRNYSGTITGVENSPGSRLM
                     SAEDSSLLASFLDRGSSEQLTNLANTSVSYYPKGEVLGLVLDLLIRAKSQGRASLDDV
                     MRRMYREFYLESAKDSYYLKGRAYTPADFERVVSETAGADMSWFFERHVRAPEQPPYD
                     EAFAAVGLRLKRTPAKQPHTAGIGFDFRAHPLLRVGYLSTGSAAQRAGLRQGDVLVTI
                     G"
BASE COUNT         1807 a         3346 c         3438 g         1861 t
ORIGIN      
        1 ccgccgacga gcgtgagctt gagggcgtcg aagaggttgg cctcgcttga gacggggcgg
       61 ttgcggagca ggaggttgat ggtctggttg atgcgcgtcg ggtcgttggt aaagtccgtg
      121 atgggcgtga cgcgcatgtc gtaggcgatg acggagacgt agtcggcggg cggcttgatg
      181 aactgcgaga ggaacatggc catcggccgc agcacctcga agagtcccgg ctcgtagttg
      241 tcggcccgca gcttctgatc catcaggttg ctgtattcca gaaccatcgc caccgtgatg
      301 ggcgcttcgg gcgtgccgaa gttcgagacc tgctgctgga cgccgtcctc gaagacggcg
      361 aagttctcct tcttcagccc cgtgatgacc tgcttcgtct tcttgtggat gacgacggcc
      421 tccagattga cgagcgccgt cgagaccttt accggctcgg tgtcttcgat ggcgtcggcg
      481 ggcggcgacg gctccacgtc gggcggctgc tcgccagggc gcggctgctg gccggggtcg
      541 gggcgcttgt ttttcttctg cggctcctgc gcgggcacgc gtctcgactg cgcgagcgcg
      601 gtgggcgcgc tcagggggcc gagcgacgcg cagaggagcg ccagagcgac gacgaggacg
      661 gaggcggtgc tgcggccttg tttgaatctc ttcatggacg aaaaaacctc ttgcgacagg
      721 gggccgggga atcattttca ggggcgacgc ttacggccga atatacgccc aaaagccgta
      781 aatcgaaaat cgttaatcgt caatcgatgg ggtgtcgctc actcaacccg agcggcgccg
      841 ctcaggtcgg gtctgactca accacgattg acgattgacg attcacgatt tacggccttt
      901 ggatgccgca actctcgcgc gtgttggccg cgcgcgccgg ccgcggtcag ggccgggcct
      961 ggccgctggt gacgggttcg gcctcgatga gggcgtcgta ggtgccggtg ttgtcggcca
     1021 ggttgccctc gttgatgccg aggaagaggc ggccgtcgcg tgtggcgtag aactcacggc
     1081 ccgcgccgat gtagacgaac tcgtcgttat cgtcgccgac gacggcgatg agcgcgcccg
     1141 tcggctcgtc gcgcatcagc ttctcgttgt cggcgacgcg cgacagcccc gtcggcgtgg
     1201 cgaagcgccc ctgcccgagc gagatgcgcc ccgacgccga gatacgcagc cgctgacctt
     1261 tacgcaccat cagccccgag tccgtccagc cgttggcggc gttgtcggcg cgcacgcgca
     1321 cgcgcacggg gaagaactgc gatgacggcg acgacgatga aggcgaaggc tgcgccgcgc
     1381 ccgtgtcaga cgtgcgggag ttgtttgccg gcggcgggtt gccggcgccc tgcatacgcg
     1441 gctgctcgga gggctgcgag gtgttgcggc tcggggccgg gttggacgcg cggttatcga
     1501 ccggggcggc cgtctgcgtg tcgccggcat cggcggtcgt ggagcctgcc ggcgcgccgt
     1561 cgaactcgat ggactcgacc tcctcgatgt aaacctgaat gcggttgcgc cggccgcgcg
     1621 ccgtcggctc catcagcacc gtgaactgct cgtcgcggaa gccgacgacc tgaccacgga
     1681 tgatcgagcc gttcttgagc cggatggtgt cggcgaggac ggccgacgaa gcggcgacgg
     1741 ccagagccac ggcgacgatg gcgcgcgaga atttaacctt catgttctcc tccgtggggc
     1801 gagcgcatcg cccccttgcg aaagcttcca gacatggcac gtgtagcaga gagacgggcg
     1861 ctttggcaag cgggggaaag ttctccgtcc ccgtggatgt gcgcggcgcg cgcgcgggtt
     1921 gcccgccact tgcgcgcccg cgcgcggcgg cgcaagtttc caccccgcca aatggaaacg
     1981 acaggccaga gcggcctgcc gtttcggcgt ctcgaatctc gtggtggagc agaagggggc
     2041 tcgaatccct agcctttgca taggacggtt attgacggtt ctcgcggaaa tagtaaactc
     2101 cccgaatgag tagcgaattg gaattcgcgg cgtgcacgcc tcgccccgtc agtcctgacg
     2161 gtttcaatcc cgaggccgtt ctgcctttcg gtcttacggc agcgcatgtt catcatgcga
     2221 tggatgactt tataaggtta atgcatgtcg ccaacgtgca actacacggc aagcaaattc
     2281 aacgactgga gactattctg gcccctgcta atttcagcgg catggtcggt gatttcatgg
     2341 cggcgaccat tcccaactat tgcgacgggc ttgccaggaa tatgtttcac aacggtcacc
     2401 cggacatgat tcccaagggg cgcttcccgg atgattctgt tcaatacgcg catgaaggga
     2461 ttgaaatcaa agcgtcgcgc tatttggccg gatggcaggg gcataacccc gagaatgcgt
     2521 ggttgatggt tttcgtcttc ggtagcaaca gacccgctga cgctctcaaa ggcatggcgc
     2581 ccaggccgtt tgaatttcag atggtcgtgg gtgcgcaaat tacggaggcg gactggggtg
     2641 ttgcagggcg tagggaggga ggccgacgta ccccgaccgc cggcgtcctc cgcacaggac
     2701 gagaaaagat gatggcgaac tggatttaca aagcccccga ccttactcga aaggaagaag
     2761 cgacccttct tgctgctcct cctccctcgt agtctggata gcggcaagtt tcggaatcgc
     2821 cttgatgctt agttcgtagt aatccacaaa tcgttcaacc cccaccgacg cgacacccat
     2881 cgcctccgcc gccgcgatgg tcgaaccacc gcccatgaaa gggtcggcga tgactccgac
     2941 gccgagcgga agcacggcgt gaacgatttg gcgcatgaac gcttgcggct taagcgtcgg
     3001 atgcccggca atctcttttt cgctccgggg agttcgccca ctcggaataa tatctgtgaa
     3061 cggtttatcg tcgggcgtac gacgtaaccc gccggtctgg aattcgcgca agcactcact
     3121 tactttcatt cctttggtaa gcggcttccg caggatgccc cacggctcat aagctgaacg
     3181 cggcaaactg gacacgttcg gaaattcatt ctccgcattc ttcgggcggt cgccgccgcg
     3241 aaacgtccgc acgaaccgaa tcaactcacc gcgaaactcc agcccgcccg caactaatgc
     3301 ggaaaagact aactgggaaa gaaaagcatt tgtggcaata aaaaggtgcg cgccgggccg
     3361 aagggagcgc tcgacaacct tcgcccattc aacaaagaag gtgtggattt gcttccgctc
     3421 tttttgattc aaggccgtga agcgggggag aggttggcgg gtgtgcccgt cgaaagacgg
     3481 cgggatgcgc cagatgccgc cattaccgtt cgcgcgtttt tctatctgat caaaatcgta
     3541 ttccttcacc ccgtagggcg gatcagtgac gataccgtgc aggctgtttt ccggaatgcg
     3601 gcttagccac tctaaacaat ccgcgtgcaa gatgagcgaa cgcccgatca cccggtgagt
     3661 gtagtcgagt gctagtcccg ttggggcttc aactagtttg gctgtggtca atggtgcatc
     3721 ccattcttgc gcggccggcc caccttgcgg tcttcgacca acttcaaatc cttttcctta
     3781 atcatgtaat cacgcccaaa cttttcggcg ggtaactgct cggcttgaat catggcaagc
     3841 acgcggcgtg gtgtgacacc taaccgctca gccacctctt ttgtcgtcag caagttcata
     3901 aaggcgttta tagcaaagag gaaactgttc cgcaagcaga cttatttctc atgaaacttc
     3961 aacccgacac ggcgaacgat cattaagatt ttaatgcatt tgctaaaggg ggtcgtaaac
     4021 agaaacagcc gcccgaaggc ggctgcaatc tgtcaatttt ttggtggagc agagggggct
     4081 cgaacccctg gcctccgcat tgcgaacgcg gcgctctgcc aaactgagct actgccccac
     4141 gcgagagttg atgataggag ccgcgccgag agactgtcaa gcacgacttc cgcatcagta
     4201 attgtagggg cagggcaagt ccgcggcccg caaggtgcgg aaggaattaa catggatgga
     4261 caggacaagc aggataagaa aatcaaatta tcaatcctgc gtatcctgtc catccatgtt
     4321 aattccttcc ttcggaaaga cgggccgcgg acttgtccct gcccctacag ttttgaaggg
     4381 cgttctaagt tgcttcaagg ctgcgcgcgg ccgcgtcgat tacgtcggcg agcagatcga
     4441 agcggcggcc cccgccctgc gccatgtcgg gacgaccgcc gccgcgtcct tcgagggtcg
     4501 cgcaggcgtc acgcatgagc gcgcccatgt cgccgggggc ggaggctgtg cgtgcgaaga
     4561 cgaggcgggc ggcgtcgccg tcgcgcgcgc cgagcagggc gacgacggag ggcgcggcgg
     4621 ggcgcgagac gagggcggag gcgatgcgcc gcaggctctc ggcgtcgcgg gcgtcgaaga
     4681 cgcgggcgac gattgacgca ccggccgcgt cgcgcgccgc tgattccgcg agcagttcat
     4741 cggcctcgac gcgcgccgcc acgtcttcga gcgcgcggtt gcggcgggca agctgcttgt
     4801 tctcctccag cagacgctcg acggagcggc cgatctcctc gcggcccacg gtgaggaggg
     4861 agctggcggc gcgcgccgct tggttcgcgc gcgcgtagtc gcgagcggcg cgcacgccgg
     4921 cggcaaactc gattcgcgtc atgcccttcg cgcgttccca tccgcgcgcg acgattgtgc
     4981 ccacctcgcc agtgcgctgc gcgtgcgtgc cgccgcaggg gctgaagtcg aagccttcga
     5041 tctcgacgac gcggagttcg ccctcgcgcg ccgagtcctt gcgtagcggc aaacgggcgg
     5101 cctcttcggc ggtgacgtgg tggacgctca cggggcggtt gccccagacg acttcgttgg
     5161 cgcgctcgac ggcccgccac acgcgctcgt ccgaggggtc tcgcaactcc acgtcgatct
     5221 cgcaggcgtc ggccgtcatg cggaagctgc gcgtctgcgc gccgtggagt tcgacgagcg
     5281 cctgcgagag tagatgctgg cccgtgtgtt gctggatgtg gtcgagccgg cgcggccaat
     5341 cgacgcggcc gtgaaccgtc tcgcccacgc cgggcgcatc gccctcgacg acgtggacga
     5401 ccttcccgtc gtcgccttcc agacaatcga cgacgcgcgc gtcgccgatg gcgccggtgt
     5461 cgaaaggctg accgccgccc gtcgggtaga aggccgtgcg gtcgagcaca agctcgcgtc
     5521 gcccgccctc gaccttgtaa gaggtgatga cgcgcgcgtc gaactccaac agatgcgagt
     5581 cggcgtagta gagtcgctcg gtggacatgg cggcaagttt atcatcaacg caaggtcaaa
     5641 gggggaaggg gtaaagggta acggggaagg ggaagaaatt agcctttcgc tttcgccctt
     5701 accctttacc ccttcccccg ccgtggctcg tgccgcgttt gtgacaaagg gcggttataa
     5761 tcccgcgcgt gacggatgcg agagacgttg acctgcggga gcttgcgcgc cgcgaggcgg
     5821 acgaggcggg gttcgccacg gggttcggcc ccgaggtcga gagcgagctg cgggagcttg
     5881 gccgtggcgg gcggggcgtc gaagggtcgg aaggcgtgcg cgacctgcgc gagcttttgt
     5941 ggtcttccat cgacaaccgg gagtcgcgcg acctcgatca ggtggagtac gccgagcgcc
     6001 tgccgggcgg cgaggtgcgc ctccgcatcg gcgtcgccga cgttgactcc tgcgtcccgc
     6061 gcggctccgc catcgaccgg cacgcgcgcg ccaacaccgt ctccgtctac acgcccggcg
     6121 ccgtcttcca catgctgccc gaacagctcg cgaccgagtt gacctcactg ctcgaagacg
     6181 cggagcgtct cgccgtcgtc acggagttaa ccgtcgccga ggacggacgg gtaatcgcca
     6241 ccgacgttta ccgcgcggcg gtgcgtaacc gccggaagct cgtctacgag ccggtcggcg
     6301 aatggctcga cgggcgctcg ggcgtccccg cggaggtcgc cggcgtcgaa ggacttgagg
     6361 agcagatacg tttacaggac gagacggccg agcgtctgcg cgcgctgcgc cggacgaacg
     6421 gcgcgctgga gttggagaca gtcgaggccg acttcgtcgt cgttgacggt cgggcggtcg
     6481 atctcgtcac gaagacgcgc acgcgctcgc aaagcatcat cgaaaacttc atggtcgcgg
     6541 cgaacacgtc gatggccgag tatctggagg cgcgcggcgt gccgtcgctg cgccgcgtcg
     6601 tgcgccggcc cgagcgttgg agtcgcatgg ctgagatcgc cgaacgcttc aatgaacgcc
     6661 tgccgcgaga gccggattcg cgcgccctgt cagacttcct cgcgcggcgg cgtcgggccg
     6721 acccggcggg ctacgcggag ctgtcgctgt cggtgatgaa gcttgtgggt agcggcgagt
     6781 atctggtcga agcgccggga cgcgagcagg aggggcactt cggtctcgcc gtggacgact
     6841 acacgcattc gacggcgccc aaccgccgct accccgacct cgtcacgcag cgttgcctga
     6901 aggcgacgct cgcgggcggc gcgtcgcctt acacggtgtc ggagttgggt gagatcgcgg
     6961 agcactgcaa ccggatggag gacgcggcgc ggaaggtgga gcggaggatg cggaaggcgg
     7021 cgacggcgat gcttctgggc gcgcgcgcgg gcgaggtgtt cgaggccgtc gtgacgggcg
     7081 cgaagccgaa gggaacgttt gtgaagctcg cggcgccgcc ggccgagggc cgcgtcgtcc
     7141 ggggcgagcg cggtctggac gtgggcgacc gcgtgcgtgt gcgtctggtt tcgaccgacc
     7201 cggagcgggg cttcatcgac ttcgcgcgcg tcagttagag ttagcgagaa cggtttgcgg
     7261 ctgaaggaag aattaacatg gatggacgag aacaagtgat aagtgatgag tgataagtga
     7321 taagaaagag aaaaacggtt ttactcttat cacttgttct cgtccatcca tgtgaaaatc
     7381 cgcctgcgcg tgcggcgcgt ggtcaatggg agaaattgag ctttgagtaa tgacccgaaa
     7441 gagatgtcgg acgaagagat tcgcgcacag gtcgtcgaac tcggcttcgg cggcgaccgg
     7501 cgtcgcttcg aggagttctg ccgcgtgttg cgcgaggggc tgcccgtggg gacgggtgtg
     7561 gccctgcgcg gcagcgtcat caccaacgaa cgctacgagg acggccgccc cttcgacgcc
     7621 aagggcaagg gcaccagcga cctcgacgtg acgctcgtcg gcgaagaggt cagggagtgc
     7681 tggaagcagg acgcctacta catccccggc cttcacacca tgccgctcca cgacgaagac
     7741 cccgactgcg cgcccaacct cgacggcctc cgccgcgagc tgcaacagat cgcccgtcgc
     7801 cccgtcagct ttcaggccac ctccaacatc gtcctcttcg cgcgcgacgt gctgatgagc
     7861 cagccctact tcatgctgat cgagcaagcg gaagcgccgt gatcctcaag ctcctcagtt
     7921 acaacatccg cttcggcggc cgcggccgcg agcgcgcgct agccgacgtg attcgcgccg
     7981 ccgcgcccga cctcgtcgtc tttcaggagg cgaccgacac gcgcgtcgtc gaggccgtcg
     8041 ccgagtccgc cgggctgccg caccacgcgg cgcggcgcga ccactccatc gcctacgccg
     8101 gccgcatcga agtcgcgcac cacgcgtggc atcaccccgc aggctcgcgc caccccttca
     8161 tggagctggt gctggccgac gaccacacgc gcgtcttcgg actccacctg agcgcgcgct
     8221 tctcgaaatg gagcgagcgg cgtcgcgcgc gcgagatgcg cgcgttgctc gacggcatac
     8281 gcgagcatca ggagggcttc catgtgctcg tcggcgactt caacacgctg gcgccgggcg
     8341 agctgttgga gttgagccgg atgccgcgtt ggattcgcgc gctcgtgtgg ctgagcgggc
     8401 gcgagctaca gcgagacacc atccagatgt tgctcgacgc cggctaccgc gatggcttcc
     8461 gcctccttca tcccgacgtg aagggctaca cgctccccgc gtgggacccc agcgtccgac
     8521 tcgattacgt cttcctgccg gcgcgcgacg ccgcgcgtct caagtcgtgc gacgtgctcg
     8581 acgcggcggg cgccgccgcg gcctccgacc acctcccgct cctcgccgag atcgacctcc
     8641 ccgtggccgc cgcctgagat cgtttttgac acgcgcggcc cgatgtgcga ataatcgcgg
     8701 tgccccggcg ctcccctgcg acagttaaaa tcaacttttc ggagtcaacc gaatgtttgt
     8761 ccgcgttcgc ctttctctgc gccgcgccgt cgtcgcgtcc gccttcgtcc tcgtccaact
     8821 aatctcttcg cccgacgccc tcgcgcagca agggacgaac gccgggccgc cccttcgcat
     8881 cagctacacg ctctcgatgc ccgcgccgga gacgcacctg ttcgaggtgc aggttgatgt
     8941 cgcgggattg cgtggcaaca cgccgcttga gttccagatg ccgcgctggt cgccgggccg
     9001 ctatgccatc ttcgacttcg ccaagaacgt gcaggaggcg cgcgccgtca cgctctgtcc
     9061 tcccggcgcg cagtgtaagg gccgggagtt ggccgtccgt cgcctggaca cgcagacgtg
     9121 gtcggtcgag acggccgacg gcggcgcgtc cttccgctac aaggtcttcg ccgacgatct
     9181 ctccggcacc ttctcgcaac tcgacacgcg ccacgccagc ttcaacggcg gctccgtctt
     9241 cgtctacgtg ggcggccaca agcaagaccc cgtgacattg aagattagag cgcccgccgg
     9301 ctggaaggtc gcgaacggct acacccgaag tcccgagcag accgagtttg ccttccccaa
     9361 ctacgacgtg ctcatcgaca cgccgaccga cgttgctccc gactggacga tggacacctt
     9421 cgacgttgac ggcaaaactt accgcgtcct agtccactcc ttcggccccg agggcggcaa
     9481 gcgcgccgcc ttcgtccgcg acatcgagaa ggtagtgcgc gagcaggtgc ggatgatgga
     9541 gggcgcgccc gactacgagc agtacacctt cctcttccac ttcgacccga ccgcccgcgg
     9601 cggcgacggc atggagcacc tcaactccac acagatcgtc tccacccgcg cgctcgccga
     9661 cgagggcgct tacgcgggca gtctctcgac ggccgcgcac gagttcttcc acgtctggaa
     9721 cgtcaagcgc ctgcgccccg cgggcctcgg cccgtgggat ttcacgcgcc ccgttgtcac
     9781 gcgcggtctc tggatcgccg agggcttcac gaattactac gagacccttt cgagacgccg
     9841 cgccggcgtc atcgacgacg cccacctgct ccgcaactac tcgggcacca tcacgggtgt
     9901 ggagaactcg ccggggagcc gcctgatgag cgccgaagat tcatctctgc tcgcctcgtt
     9961 cctcgaccgt ggcagcagcg agcagctcac gaacctcgcc aacacgtccg tcagctacta
    10021 cccgaagggc gaagtgcttg gcctcgtcct cgacctcctc atccgcgcca agtctcaggg
    10081 ccgcgcctcg ctcgacgacg tgatgcggcg catgtatcgc gagttctatt tagagagtgc
    10141 gaaagattct tactacctga aggggcgcgc ctacacgccc gccgacttcg agcgcgtcgt
    10201 ctcggagacg gccggcgccg acatgagttg gttcttcgag cgccacgtgc gcgcgccgga
    10261 gcagccgccc tacgacgagg cgtttgccgc cgtcggcctt cgcctcaaac gcacgcccgc
    10321 gaagcagccg cacacggccg gaatcggctt cgacttccgc gcccacccgc tactgcgcgt
    10381 cggctatctc agcaccggct ccgcggccca gcgcgccgga ctccgacagg gcgacgtgct
    10441 cgtcaccatc gg
//
LOCUS       DASVES010000385         3912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_903895, whole genome shotgun sequence.
ACCESSION   DASVES010000385 DASVES010000000
VERSION     DASVES010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903895"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2997)
                     /locus_tag="VF240_15670"
     CDS             complement(139..2997)
                     /locus_tag="VF240_15670"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HEX7176703.1"
                     /translation="MRHVALAPRRLYVLLSFLLVMLAPATARAVPIVALMDFNRLVQF
                     DSSSPGTIISSGLVTGIIPGDRLIAIDFRPATGQLYGFSTGDRLYVIDPQTRAVIRTL
                     GPISPTINGNAIALDFDPSRDRLRVINDDAGRQNFLVDPDPATGGGVSVETPSEFAPD
                     DVNALARSTIHGLAYDNNFLGAAHSRLWAIHRGVGSNVPYLVTIAPAGGGQIRTVGPV
                     LGVTDAAHVTSDIGFDIAPVGGSAYAVLKINSPTITVTHLYEVNLATGAATQLGAIGE
                     PGTVTDIAVVPPPLIVYAVNEQNELIKFNSATPSNIISRVKITGLLDNEEIYDIDFRP
                     TTDFRPGREELYGLGRAGSGATATSSRIYVINPETGVAAPPSVTNPLMPALAGVPSFD
                     FNPIGESLRVVTSTEQNLMISTDLGELSFMATPLAYVPGDPNAGRDPNVLAAAHSNNF
                     PGATSTTLYVIDRDQPGFGAHLSRVGDAGGARLAANSGRMFTVAQSVGFGVPVDDIGF
                     DIAPGVEGTALLSTISMDEAFSRLSVFGTDRAGGGHVGPIGQTGALERLRDLAVAPTA
                     TFRFETDITQIVEDCTSVAVNVLRVGDASNEATVDYATVERMGAINQRRNYTAARGTL
                     HFAAGQTSAVIKVLISEDAHLEKGIDHGVPFNGIFFELMLTNPSNGHTLGDAGNRFTN
                     VHVLDDEDTGAEPNPINDSHTFVCQHYHDFLGREPDAAGLAFWTNNIESCGASAACRA
                     VKRIETSAAFFLSIEFQQTGFLVYRTHAAAFGSTRVGGAVPLALEEFLFDTERVARGV
                     IVGPGDWQARLENNRRAYFDEFTQRPAFEAVYPRALAAAGFVDALNANTSGSLAPAER
                     DALAAGLETGAETRATVLRKVVENAAFRQRELNRAFVLTQYFGYLRRNPNDFPDSDFS
                     GYNFWLAKLNQFGGDFRAAEMVKAFITSGEYKSRFGNP"
     gene            complement(3211..>3912)
                     /locus_tag="VF240_15675"
     CDS             complement(3211..>3912)
                     /locus_tag="VF240_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7176704.1"
                     /translation="IIVPGNHDVPYYRVWERFLAPLDKYRRFITEDLEPFYSDDEIAV
                     LGINTARSLTFKNGRVNIRQIEKISEHLCPFADDVTKIVVTHHPFDVPEGHDESDLVG
                     RAARAMEALARCGADLLLAGHLHVGRTGHTATRYKIKGHSALVVQAGTATSTRHRGET
                     NSFNVVRVKHPYVNVERLVWQPEHASFTPASAETFQHTPDGWARLPDEVAAGITFEHR
                     SRTLQPDDPTGSAGS"
BASE COUNT          590 a         1106 c         1535 g          681 t
ORIGIN      
        1 aggttctttc gcccctccgg ggcttcagat tttgtttgac gactgttcca cgggctaacg
       61 cccgtggcta cattcttccg ccgcctccgg cggctcgctg ccgcccgcga ctcaactccc
      121 tcatttagac ggcggccgtt acgggtttcc gaaccggctc ttgtactcac cgctcgtgat
      181 gaaggccttg accatctcgg ccgcgcggaa gtcgccgcca aactgattga gcttcgcgag
      241 ccagaagttg tagccggaga aatcgctgtc ggggaagtcg ttcgggttgc gacgcagata
      301 gccgaagtac tgcgtcagga cgaaggcgcg gttcagctcg cgctggcgga aggccgcgtt
      361 ctcgacaact ttcctgagca cggtggcgcg cgtctcggcc cctgtctcca gcccggcggc
      421 gagcgcgtca cgttcggccg gcgcgagcga gccggaggta ttggcgttga gggcatcgac
      481 gaagccggcc gccgccagcg cgcgcgggta gaccgcctcg aaggccgggc gctgcgtgaa
      541 ctcgtcgaag tacgcgcgcc ggttgttctc cagacgcgcc tgccagtcgc ccggcccgac
      601 gatgacgccg cgcgcgaccc gctccgtgtc gaacaggaac tcctctaacg cgagcggcac
      661 agcgccgccg acgcgtgtgg agccgaaggc cgcggcgtgt gtgcggtaga cgaggaagcc
      721 ggtctgctgg aattcgatgg aaaggaagaa ggcggccgac gtttcgatgc gtttgacggc
      781 gcgacaggcg gccgaagcgc cgcacgactc gatgttgttc gtccagaagg cgaggcccgc
      841 cgcgtcaggc tcgcggccga ggaagtcgtg gtagtgctgg cagacgaagg tgtgcgagtc
      901 gttgatgggg ttcggctccg cgcccgtgtc ctcgtcgtcg aggacgtgga cgttcgtgaa
      961 ccgattgccg gcgtcgccga gcgtgtggcc gttcgatggg ttcgtgagca tcagctcgaa
     1021 gaagatgccg ttgaagggca cgccgtggtc aatgcccttc tcaaggtggg cgtcctcgct
     1081 gatgaggact ttgatgacgg ccgaggtctg gccggcggcg aagtggagcg tgccgcgcgc
     1141 cgccgtgtag ttgcggcgtt ggtttatcgc tcccatgcgc tcgacggtcg cgtaatcaac
     1201 ggtcgcctcg ttcgacgcgt cgcccacgcg caggacgttg acggcgacgg atgtgcagtc
     1261 ctccacgatc tgcgtgatgt cggtctcgaa gcggaaggtg gcggtcgggg ccaccgcgag
     1321 gtcgcgcaga cgctcaagcg cgccggtctg tccaataggg ccgacgtggc cgccgcccgc
     1381 gcggtcggtg ccgaagacgg agagacgcga gaaggcttcg tccatcgaga tggtcgagag
     1441 cagggccgtg ccctcgacgc cgggggcgat gtcgaagccg atgtcatcga cggggacgcc
     1501 gaagccgacg ctctgggcga cggtgaacat ccggccgctg ttggcggcga gtcgcgcgcc
     1561 gccggcatcg cccacgcggg agaggtgcgc gccgaagccg ggctggtcgc ggtcgatgac
     1621 gtagagcgtg gtggaggtgg cgccggggaa gttgttcgag tgggcggcgg ccagcacgtt
     1681 cgggtcgcgt cccgcattgg ggtcgccggg gacgtaggca agcggcgtcg ccatgaagct
     1741 gagttctccg agatcggtgc tgatcatcag gttctgctcg gtgctggtga cgacgcggag
     1801 gctctcgcca atggggttga agtcgaacga cgggacgccc gcgagcgcgg gcatcagcgg
     1861 gttggtgacg gagggcggcg cggccacgcc cgtctccggg ttgatgacgt agatgcggct
     1921 gctcgtggcg gtcgcgcccg agccggcgcg gcccagtccg tacagttcct cgcggccggg
     1981 gcggaagtca gtcgtggggc ggaagtcgat gtcgtaaatc tcttcgttgt cgagcaggcc
     2041 cgtgatcttc acgcggctga tgatgttcga gggggtcgcg ctgttgaact tgatgagttc
     2101 gttctgctcg ttgacggcgt agacgatgag cgggggcggg acgacggcga tgtctgtgac
     2161 cgtgcccggc tcgccgatgg cgccgagctg cgtggccgcg cctgtggcga ggttgacctc
     2221 gtagaggtgc gtgacggtga tcgtggggct gttgatcttc aggacggcgt aggcggagcc
     2281 gccgacgggc gcgatgtcga acccgatgtc gctcgtgacg tgggcggcat cggtgacgcc
     2341 gagcacgggg ccgacggtgc gaatctgccc gccgccggcg ggcgcgatgg tgacgagata
     2401 ggggacgttt gagccgacgc cgcggtggat ggcccagagt cgggagtggg ccgcgccgag
     2461 gaagttgttg tcgtaggcca gcccatggat ggttgagcgc gccagcgcgt tcacgtcgtc
     2521 gggggcgaac tcggacggcg tctccacgct cacgccgccg cccgtggccg ggtcggggtc
     2581 aacgaggaag ttctggcggc ccgcgtcgtc gttgatgacg cggaggcggt cgcgcgaggg
     2641 gtcgaagtcg agggcgatgg cgttgccgtt gatggttggg ctgatgggac cgagcgtgcg
     2701 aatgacggcg cgcgtctgtg ggtcgatgac gtagaggcgg tctccggtgc taaagccata
     2761 gagctgcccg gtggccgggc ggaagtcgat ggcgatgagc cggtcgccgg ggatgatgcc
     2821 ggtgaccaga cccgaagaga tgatcgtgcc gggcgagctg ctgtcgaatt gtacgagccg
     2881 gttgaagtcc atgagcgcga cgatgggcac ggcgcgcgcg gtcgcgggtg cgagcatcac
     2941 gagaaggaag gagaggagga cgtacagccg gcggggcgcg agggcgacgt gacgcatgga
     3001 acgactcctt gttggcaatg tgaatgcgga caacttgagc cgacaacttg agccgaccgc
     3061 gcagggcctt accgggcgcc tcgcgacggc cgtgagttcg agcatgatag ttctcgacga
     3121 atgggctgac cgagtttcgg ccgggcgacg gggagtcggg gaaaagtcta tggccggtgg
     3181 cgcgtgtaag tcaacggctt tgttttcggc tcaggagccg gccgagcctg tggggtcgtc
     3241 gggctggagg gtgcggcttc ggtgctcgaa ggtgatgcca gcggcgactt cgtcggggag
     3301 acgcgcccag ccgtcgggcg tgtgctggaa ggtctcggcc gaggcgggcg tgaacgaggc
     3361 gtgttcgggt tgccagacga gacgctcgac gttgacgtaa gggtgcttga cgcggacgac
     3421 gttgaaggaa ttggtctcgc cgcggtggcg cgtggaggtg gccgtgccgg cctgaacgac
     3481 gagcgcggag tggcccttga tcttgtagcg cgtggcggtg tggccggtgc ggccgacgtg
     3541 gaggtggccg gcgagcagga ggtcggcgcc gcagcgggcg agcgcctcca tcgcgcgcgc
     3601 ggcgcggccc acgaggtcgc tctcgtcgtg cccctcgggc acgtcgaacg ggtggtgcgt
     3661 gacgacgatc ttggtcacgt cgtcggcgaa ggggcacagg tgttcgctga tcttttcgat
     3721 ctgccggatg ttgacgcgcc cgtttttgaa cgtgagcgaa cgcgcggtgt tgatgccgag
     3781 gacggcgatc tcgtcgtccg agtagaaggg ttcgaggtct tcggtgatga agcgccggta
     3841 cttgtcgagc ggggcgagga agcgctccca gacgcggtag tacggcacgt cgtggttgcc
     3901 ggggacgatg at
//
LOCUS       DASVES010000386         7340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1620469, whole genome shotgun sequence.
ACCESSION   DASVES010000386 DASVES010000000
VERSION     DASVES010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620469"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2858
                     /locus_tag="VF240_15680"
     CDS             <1..2858
                     /locus_tag="VF240_15680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7176705.1"
                     /translation="QGFATLVQESFPLTVGQAVSLNLRMKVSQVEERVTVTAVPTVDV
                     VKTESSTTLNERAISNLPVLGRKFEDLLTLTPGVSIVQGPDGDEINFVGQRGVFNNIS
                     LDGGDYNNGFFGEQAGGQRAAIDIPLDAVQEFQVIATGASAEFGRTAGGIVNVITKSG
                     GNDFHGSLFHFQRLEALTANTSDGRSLTDFHREQFGGTVGGPIKRDRTFFFAAVESIR
                     ENLTRGNLSESLGACPATAPTILANEALINTNTECQRLALLDFFRTTRQQEEGLPTER
                     PISNTAVLGKLDSDITPNNKLSLSYNFDYSKNTNQTFDVTTYGNSANGIEGPSKIGVF
                     NLNLFSTVTPNVVNEAHFTYSRENRPRAAVDSNVPADTAMGFATTFRFGAPFFLQPTV
                     DEIFWRTQLRDNVSVIAGAHTVKVGGEWIHSKNIQTFRGFFTGRYIFDSVAGFLRYAS
                     PASLGPGFGPRTMGCSNGTYVDVLSPCPAGSTPTGGPLLLYLQDAPTGNSDIPPGFSD
                     IKNEDLAVFAQDSWKVRPNFTLNYGLRWEAQIFPDPVVPPAETAYGRFLSDPRFPSDG
                     TLPNQKSQFQPRVGFAWDIGGKGKSVLRANYGIYYARQNMLTQVGSITTNGVQQQTIF
                     RNTGLIGLLLGFGIPQSAVAPTWPGLVTPPVLPAGTFNDFTGVRVFSRDYQNPRIYTT
                     NFAFEQELATNWAAYVDFTHAKGVHLPRFINVNIGDLPAVLPANGNSVVYTGAPPFGP
                     QLGDVFVASSVGKSLYRGLTLGVRKRFSRGYQLEGNYVFSKDLDDDSNERDPFTDRTF
                     NRFDLQKDYALSDRDIRHRFNFYTYMELPGGFDANLRLQARTAQPTTFNPLGTGTGAP
                     CSESNSLTRVVAGIDCGRNHLRKNNEFFSFDWRLQRPFRFSENVALIPVVEMFNTFNT
                     ENNVNPLITPGLFNFDGFLRQGVGDPRIVQLALRFLF"
     gene            complement(2953..3630)
                     /locus_tag="VF240_15685"
     CDS             complement(2953..3630)
                     /locus_tag="VF240_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX7176706.1"
                     /translation="MEGTGVVPLTRVKICCIGSVEEARLAVEYGASAVGLVSEMPSGP
                     GVIDEGLIAEIAATVPPGVATFLLTCKDDPAVIVEQQRRTRVGVVQICDRLEAGAHLK
                     LRRAMPGVALVQVVHVTGPESVEEAASVAPHVDAILLDSGNQSLAVKELGGTGRVHDW
                     ELSRRIRATAGVSVFLAGGLNAGNVAEAVSRVRPFGVDVCGGVRTDGRLDEGKLSDFF
                     RSVAAAG"
     gene            complement(3631..4155)
                     /locus_tag="VF240_15690"
     CDS             complement(3631..4155)
                     /locus_tag="VF240_15690"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7176707.1"
                     /translation="MLRANIFAALLMLACLPASARAQDVKKAPDFALRDLRGRSVRLR
                     DFRGRVVLLNFWATWCPPCRAEAPELVHWQRKHGGAGLQVVGVTQPPASRAAVRRFVR
                     RAKVNYPVLFGTRGTRTLFDAGETLPVTAVIDREGNLSALIEGIILPEEFEQKVRPLL
                     YPRARFKRGTGRAL"
     gene            complement(4165..5526)
                     /locus_tag="VF240_15695"
     CDS             complement(4165..5526)
                     /locus_tag="VF240_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7176708.1"
                     /translation="MSRLEFAPKLKEIEIVDIKKGLGLFKPKPGRQVSFAALKEALKK
                     AGYTLASAEITVAGTLAREGKNWRVVAEGTGQHFALEGTDVGRLLTGAEEGSRVEAAG
                     DWQTVGEKTNARERLAPRLLKKTGGAEETKPAARAREKSLTAGAYFVGAHFVGGLFES
                     ETHFDAPAATKSAPPMAKPAAPIRTTSPGLTVYKGGAVTPRLYFVKQHAGGLDVRRQV
                     LDLSVSYTPTPRLQLEAEAPLARTSFDDGEMEGSGFGLGNVTLWAKYRFYRSVKTYGD
                     RQASARVGLELPTGKKDAPTSAQAGVPDFVRQQLTPISGGFAPHFDVAFSQAGGRLIF
                     GGNVEGVVRAERAGFRTGHELRANTDVEYVLLPRRYDTPGGELFVILETNFVWRGRGR
                     MGGAVVDDSKATEFYLSPGLQYAARPRLVFEGSLQLPVVRHTGAQLLKTDYNLLLGMK
                     YLF"
     gene            complement(5548..5670)
                     /locus_tag="VF240_15700"
     CDS             complement(5548..5670)
                     /locus_tag="VF240_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176709.1"
                     /translation="MKRLPKRLACVAALLLACAASAAAQVSKVEMRIDGYLCGN"
     gene            5896..7194
                     /locus_tag="VF240_15705"
     CDS             5896..7194
                     /locus_tag="VF240_15705"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX7176710.1"
                     /translation="MSANQKDTLTITDNRTGQQYEVPITDGTIRAMDLRQIKSGDEDF
                     GLMTYDPAFMNTASCKSTITYIDGDRGILEYRGYPIEQLAERSSYLEVAYLLLHGELP
                     TAEQLANWTRHITFHTFINENIKKVLDGFHHSAHPMGMFEAMVAALSTFYPEAKNIND
                     EPCRQKQIYRLIAKVPTIAAFAYRHRIGLPYAYPDNELSYEGNFLNMMFKTTELKYQP
                     NAVLERALSVLFILHADHEQNCSTNAMRSIGSAETDPYSSLAGASAALYGPLHGGANE
                     AVLRMLKEIGSVASIPAFIQRVKSGDGRLMGFGHRVYKSYDPRAKIIKKTAYEVFEVT
                     GKNPLLDIALELERIALQDDYFVTRKLYPNVDFYSGLIYQAMGFPVEMFPVLFAIPRT
                     SGWIAQWEEMLLDPDQKIARPRQIYVGATRRDYVPLEQRT"
BASE COUNT         1326 a         2703 c         2082 g         1229 t
ORIGIN      
        1 agcagggttt cgccaccctc gtgcaggaga gttttcccct cacggtcggg caggccgtct
       61 cgctgaattt gcggatgaag gtctcgcagg tcgaggagcg cgtgaccgtc accgccgtcc
      121 cgacagtgga cgtcgtgaag accgagtcga gcacaacctt gaacgagcgc gccatctcga
      181 acctgcccgt cctgggccgc aagtttgaag atttgctgac gctcaccccc ggcgtctcca
      241 tcgtgcaggg gccggacggc gacgagatca acttcgtcgg ccagcgcggc gtcttcaaca
      301 acatcagtct cgacggcggc gactacaaca acggcttctt cggcgagcag gccggcggcc
      361 agcgcgccgc catcgacatc cccctcgacg ccgtgcagga gtttcaggtc atcgccacgg
      421 gcgcctcggc cgagttcggc cgcacggcgg gcggcatcgt caacgtcatc accaagtcgg
      481 gcggcaacga cttccacggc tcgctcttcc acttccagcg gctcgaagca ctcaccgcca
      541 acacctccga cgggcgctca ctcaccgact tccaccgcga gcagtttggc ggcaccgtcg
      601 gcggccccat caaacgcgac cgcacatttt tcttcgccgc cgtcgagagc attcgcgaga
      661 acctgacgcg cggcaacctg agcgagtcgc tcggcgcctg ccccgcgacg gccccgacca
      721 tcctcgccaa cgaggcgctc atcaacacca acaccgaatg ccagcgtctc gccctgctcg
      781 acttcttccg cacgacgcgc cagcaggagg agggcctccc caccgagcgc cccatcagca
      841 acacggccgt gctcggcaag ctcgactctg acatcacgcc caacaacaag ctctcgctct
      901 cttacaactt cgactattcg aagaacacca accagacctt cgacgtgacg acctacggca
      961 actccgccaa cggcatcgag gggccgtcga agatcggcgt cttcaacctc aacctcttta
     1021 gcaccgtcac gccgaacgtc gtcaacgagg cccacttcac ctacagtcgt gagaaccgcc
     1081 cgcgcgcggc cgtcgattca aacgtacccg cggacaccgc gatgggcttc gccaccacct
     1141 tccgcttcgg cgcaccgttc ttcctccagc cgaccgttga cgaaattttc tggcgcacgc
     1201 agttgcgcga caacgtctcg gtcatcgccg gcgcgcacac cgtcaaggtc ggcggcgagt
     1261 ggattcacag caagaacatc cagacgttcc gcggcttctt cacgggccgc tacatcttcg
     1321 actcggtcgc cggcttcctg cgttacgcct cgcccgcgtc cctcggcccc ggcttcggcc
     1381 cgcgcacgat gggctgctcg aacggcacat acgttgacgt tctctcacct tgccccgccg
     1441 gctccacccc gacgggcggc ccgctccttc tctacttaca ggacgcgccc acgggcaact
     1501 ccgacatccc gcccggcttc tccgacatca agaacgaaga cctcgccgtc ttcgcgcagg
     1561 actcctggaa ggttcgtccg aacttcacgc tcaactacgg cctgcgctgg gaggcgcaaa
     1621 tcttccccga ccccgtcgtc cctccggccg agaccgctta cggacgcttc ctctccgacc
     1681 cgcgtttccc ctccgacggc acgctcccca atcagaagag ccagttccag ccgcgcgtcg
     1741 gcttcgcgtg ggacatcggc ggtaagggca agtcagtcct gcgcgccaac tacggcatct
     1801 actacgcgcg ccagaacatg ctcacgcagg tcggctccat caccaccaac ggcgtgcagc
     1861 agcagaccat cttccgcaac accggactca tcggtctcct gctcggcttc ggcatcccgc
     1921 agtcggccgt cgcgcccacc tggccggggc tggtgacgcc gcccgtcctc ccggccggca
     1981 ccttcaacga cttcacgggc gtgcgcgtct tcagccgcga ctaccagaac ccgcgcatct
     2041 acaccaccaa cttcgccttc gagcaggagc tggccacgaa ctgggccgcc tacgttgact
     2101 tcacacacgc caagggcgtc cacctgccgc gcttcatcaa cgtcaacatc ggcgacctgc
     2161 ccgcggtgct gcccgccaat ggcaactcgg tcgtctacac gggcgcgccg cccttcggcc
     2221 cgcagctcgg cgacgtgttc gtggcgtcga gcgtcggcaa gtcgctctac cgcggactca
     2281 cgttgggcgt gcgcaagcgc ttcagccgcg gctaccagct cgaaggcaac tacgtcttct
     2341 ccaaagacct cgacgacgac tccaacgagc gcgacccgtt caccgaccgc accttcaacc
     2401 gcttcgacct ccagaaagat tacgcgctct ccgaccgcga catccgccac cgcttcaact
     2461 tctacaccta catggagctg cccggcggct tcgacgccaa cctccgcctt caggcgcgca
     2521 ccgcgcagcc gacgaccttc aacccgctcg gcacggggac gggcgcgccg tgcagcgagt
     2581 cgaactcgct gacgcgcgtc gtggcgggca tcgactgcgg acgcaaccac ctgcggaaga
     2641 acaacgagtt cttctccttc gactggcggc tccagcgccc gttccgcttc tcggaaaacg
     2701 tggcgctcat ccccgtcgtc gagatgttca ataccttcaa caccgagaac aacgtcaacc
     2761 cgctcatcac gcccggactc ttcaacttcg acggcttcct ccgtcagggc gtcggcgacc
     2821 cccgcatcgt gcagttggca ctgcggttct tgttctgacc ggacgcggca gggaacacgg
     2881 atgggacgga tgaacacgga tcaaatccgt cttcatccgt cccatccgtc ttatccgtgt
     2941 tccctttatg atctaccctg ctgcggcaac gcttctgaaa aaatccgaca acttcccttc
     3001 gtccaaacgc ccgtccgtcc gcacgccgcc gcacacgtca acgccgaacg gtcgcacacg
     3061 gctgaccgct tcggccacgt tgcccgcgtt gagaccgccg gcgaggaaaa cgctcacgcc
     3121 cgccgtcgcg cgtatccgcc ggctgagttc ccaatcgtgc acgcgccccg tcccgccgag
     3181 ttccttgacg gcgagcgact gattgccgga gtcgagcagg atggcatcga cgtgcggcgc
     3241 caccgacgcg gcctcctcaa ccgactccgg ccccgttacg tggacgacct gcacgagcgc
     3301 cacacccggc atcgcccgcc tgagcttcag gtgcgcgccc gcttcgagcc ggtcgcaaat
     3361 ctggacgacg cccacgcgcg tccgccgctg ctgctcgacg attacagcgg ggtcgtcctt
     3421 gcacgtcagc agaaaagtag ccacgccggg cggcaccgtc gccgcgatct ccgcgatcaa
     3481 cccttcgtcg atgacgccgg ggccgctcgg catctcggag acgaggccca ctgccgacgc
     3541 cccatactca accgccagac gcgcctcctc cacactcccg atgcaacaga ttttcacgcg
     3601 cgtcaacggc acgactcctg tcccctccac tcacaacgcc ctgcccgtcc cgcgtttgaa
     3661 tcgggcgcgc gggtagagca gtggcctcac cttttgctca aactcctcgg gcaggatgat
     3721 gccctcgatc agcgcgctca ggttgccctc gcggtcgatg acggcggtga cgggcaacgt
     3781 ctcgcccgcg tcgaagagcg ttcgcgtccc ccttgtgccg aacaggacgg gatagttgac
     3841 cttcgcgcgg cgcacgaaac gccgcacggc cgcgcggctc gccggcggct gcgtgacgcc
     3901 gacgacttgc agccccgcgc cgccgtgctt cctttgccaa tggacgagtt cgggcgcctc
     3961 cgcgcggcat ggcgggcacc acgtcgccca gaagttgagg agcactactc ggccgcggaa
     4021 gtcgcgcaga cgcacgcttc gcccgcgcaa atcgcggagc gcgaagtcgg gcgccttctt
     4081 cacgtcctgc gcgcgcgcgc ttgctggaag gcacgcgagc attagtaacg cggcgaagat
     4141 gtttgccctg agcacggttc gatttcagaa aagatacttc atgccaagca gcaggttgta
     4201 gtcggtcttc aacaattgcg ccccggtgtg tcggacgacc gggagttgga ggctgccctc
     4261 gaagacgagg cgcggtcggg ccgcgtactg aagccccggc gacaggtaga actccgtcgc
     4321 cttgctgtca tcgacgaccg cgccgcccat ccggccccgc ccgcgccaca cgaagttcgt
     4381 ctccagaatg acgaacaact cgccgcccgg cgtgtcgtac cggcgcggca ggagcacgta
     4441 ctccacgtcg gtgttggcgc gcagctcgtg gcccgtgcgg aagcccgcgc gctcggcgcg
     4501 cacgacgccc tcgacgttgc cgccgaagat caagcgcccg cccgcctgcg agaaggccac
     4561 gtcgaagtgc ggggcgaagc cgccgctaat cggcgtgagc tgctggcgga cgaagtccgg
     4621 tacgcccgcc tgcgcggacg tgggcgcgtc cttttttccc gtcggcagct caagcccgac
     4681 gcgcgccgac gcctgccggt cgccgtaggt tttcacagac ctgtagaagc gatacttggc
     4741 ccacagcgtg acgttgccca gaccgaagcc cgagccttcc atttccccgt cgtcgaagga
     4801 ggtgcgcgcg aggggcgctt ccgcttcgag ctggagacgc ggcgtcggcg tgtaggagac
     4861 gcttaggtcc aacacctgcc ggcgcacgtc gaggccgccg gcgtgttgct tcacgaagta
     4921 gaggcgcggc gtgacggcgc cgcccttgta gacggtcagc cccggcgagg tcgtgcgaat
     4981 cggcgcggcg ggcttcgcca tcggcggcgc cgactttgtc gcggcgggcg cgtcaaagtg
     5041 cgtctcactc tcgaagagac cgcccacgaa gtgcgcgccg acgaaatacg ccccggccgt
     5101 cagggacttt tcacgcgcgc gcgcggcggg ctttgtctcc tccgccccgc ccgtcttctt
     5161 caacagccgc ggggcgaggc gctcgcgcgc gttcgtcttc tccccgaccg tttgccagtc
     5221 gcccgcggcc tcgacgcgcg agccttcctc ggcccccgtc agcaggcgcc ccacgtccgt
     5281 cccttcgagg gcgaagtgct gacccgtgcc ttcggcgacg acgcgccaat ttttaccctc
     5341 ccgcgcgagc gtgcccgcga cggtaatctc ggccgaggcg agcgtgtagc cggccttctt
     5401 cagcgcctcc ttcagcgcgg cgaacgaaac ctgccggccg ggcttcggct taaacaagcc
     5461 gaggcccttc ttgatgtcta cgatctcgat ctctttcagc ttcggtgcga actcaagacg
     5521 gctcacggcc ttctggatgt tgaagactca attgccgcac aggtagccgt cgattcgcat
     5581 ctccacttta ctgacctgcg ccgcggcgct cgccgcgcac gcgagcagca gcgccgccac
     5641 gcaggcgagc ctcttgggta gtctcttcat caaacccttc tccttaacga tggccaggat
     5701 taattcattg tcaacaatgg atagaccctg cgcgaaagta taaaccctgt agccatcttc
     5761 agagtcaagc aaccacacgc ggccccgcgc gctcgccgcc ccagcgcctt tgaatagttg
     5821 ccggaaaagc aatatgctag gctcaaacag tatccccaaa aagtgagacc ggacgcgccg
     5881 aggagaggac gagccatgag tgcgaaccag aaagacacgt tgaccatcac tgacaaccga
     5941 acgggccagc agtacgaggt gcccatcacc gacggcacga tccgcgcgat ggacctgcgc
     6001 cagataaaat ccggggacga agacttcggc ctgatgacct acgaccccgc cttcatgaac
     6061 accgcctcct gcaagagcac catcacctac atcgacggcg accgcggcat cctcgaatac
     6121 cgtggctacc ccatcgaaca gttggccgaa cgtagcagct atctggaggt cgcctacctt
     6181 cttctgcacg gcgagctgcc caccgccgaa cagctcgcga actggacgcg ccacatcacc
     6241 ttccacacct tcatcaacga gaacatcaag aaggtcttgg acggcttcca ccacagcgcg
     6301 cacccgatgg gcatgttcga ggcgatggtc gccgcgctct cgaccttcta ccccgaggcg
     6361 aagaacatca acgacgagcc gtgccggcag aaacagattt accgactcat cgccaaggtg
     6421 ccgaccatcg ccgccttcgc ctaccggcac cgcatcggtc tgccctacgc ctaccccgac
     6481 aacgaactct cctacgaggg caacttcctc aacatgatgt tcaagacgac ggagttgaag
     6541 tatcagccca acgcggtgct tgagcgcgcc ctgagcgtcc tcttcatcct ccacgccgac
     6601 cacgagcaga actgctcgac caacgccatg cgctccatcg gctcggccga gaccgacccc
     6661 tactcctctc tcgccggcgc ctcggccgcc ctctacggcc cgctccacgg cggggccaac
     6721 gaggccgtgc tccgcatgct caaggagatc gggtcggtcg ccagcattcc cgcgttcatc
     6781 cagcgcgtca agagcgggga cgggcggctc atgggcttcg gccaccgcgt ctacaagtcc
     6841 tacgacccgc gggcgaagat catcaagaag accgcctacg aggtgttcga ggtcacgggc
     6901 aagaacccgc tgctcgacat cgcactcgag ctcgagcgga tcgcgctgca ggacgactac
     6961 ttcgtgacgc gcaagctgta tccgaacgtg gacttctact cggggctgat ctatcaggcc
     7021 atgggtttcc ccgtcgagat gttccccgtc ctctttgcca tcccgcgcac ctccggctgg
     7081 atcgcgcagt gggaggagat gctgctcgat cccgatcaga agatcgcccg tccgcggcag
     7141 atttacgtcg gcgccacgcg gcgggactac gtgccgctgg agcaacggac gtaaaggctc
     7201 aggaatcagg gaaccgggat cagcgtaaga aatcaaccga gccgtgatgc ggtttctctt
     7261 agcagcactg gtgctgtttg cgatgcagtc tcagcgccct ggttcccctg attccagatc
     7321 cctgatccct gattcccata
//
LOCUS       DASVES010000387        23768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_986971, whole genome shotgun sequence.
ACCESSION   DASVES010000387 DASVES010000000
VERSION     DASVES010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23768
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986971"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1063)
                     /locus_tag="VF240_15710"
     CDS             complement(146..1063)
                     /locus_tag="VF240_15710"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7176711.1"
                     /translation="MQLRLLLSHGAFVALLCLLLCPVAAHAQVSQNCPLPAPTGYVND
                     YAGVIDAASKERMEAVLTNLKGRAEVEFAVVTVKTTGERAIFDYSLALARCWGLGSKE
                     GDKNALLLVVAVDDRKFQPQVSRHLEGDLPDGLVTTILREKLPAAFRAGEYGRGLSEA
                     VLTLVATLADKRGFSIEGIDQSYVYRPRAQGPTRGRPVPVGRNSLGLGACCLIGFVLL
                     LVLSSIGRRGGGGGRGGRGGGWGGGGGLLNALILGQVLSNLGGRGSSGWGGGGGGGGS
                     SSGWGGGGGFGGFGGGGDFGGGGGPGGDW"
     gene            complement(1164..1955)
                     /locus_tag="VF240_15715"
     CDS             complement(1164..1955)
                     /locus_tag="VF240_15715"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX7176712.1"
                     /translation="MNFGKILFFGLLVALGNVLGGLILARPGATVRSLRTLKYLVALG
                     AGFMLAAIFIEIMPNVVELWSASGAGGSRGEGGVLSAMVLLLAGYLLVHFVEHTIAPH
                     FHFGAETHPEALIRPSAAYTAIGGLTIHTFFDGVSVAAAFLVGHKLGLLVFLAVLLHK
                     VPEGFTAASIVLASGRSTRSAFLSTLVIGAATLAGVLTVALLQSRVTDAVLYALPFSA
                     GVTLYVAASDLIPEVNHLEEKNPLVSLVVFVGVALFYLFHLLIEG"
     gene            2253..2693
                     /locus_tag="VF240_15720"
     CDS             2253..2693
                     /locus_tag="VF240_15720"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7176713.1"
                     /translation="MLRGNYTARIDTKGRLKVPTAFRRYIEDKYGAEFYVTSLSGDCA
                     RIYPLPEWETIEQRLALLPSMDPARRKFLDRTNYYGQQAAMDAQGRVLIHPLLRKSAG
                     VVGDVAVLGYLTYLEVWELERFERQLLSDPYREEDEAAIARLGI"
     gene            2804..3760
                     /gene="rsmH"
                     /locus_tag="VF240_15725"
     CDS             2804..3760
                     /gene="rsmH"
                     /locus_tag="VF240_15725"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7176714.1"
                     /translation="MAETGGGPVAPHRPVLLTEVVALLAPERGGLFIDATLGLGGHSE
                     ALLEASPDVRVLGLDRDREALRLATERLARFGSRFRAAHANFKEVARVMSDAGERDAA
                     GLLADLGVSSLQFDTAGRGFSFRHDAPLDMRMDAEGQEETAAELLAQLPEEEIARVIY
                     EYGEERRSRRIARWIVERRERGAPIQTTTELAELVARAVGHKRGERIHPATRTFQALR
                     IAVNRELEGLGEFIERAVDLLQPAGRLAVISFHSLEDRVVKRTLQRLAGKCECDPRFP
                     VCTCGARRAVELLTRRPVVPTAEEAEDNPRARSAKLRACQKL"
     gene            3862..4338
                     /locus_tag="VF240_15730"
     CDS             3862..4338
                     /locus_tag="VF240_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176715.1"
                     /translation="MRRVPQQHANSYVRRERDPRTLARQALLLASCVVLAAGFVLAAR
                     QKIAAVQYGYRSEELRRERERLLEEQHRLLVAVEESSSPVHLERAARTLGLQTARAAQ
                     IGVSETVTPERGGVEDMSGAARTGAEGADSKPRRKSGGVTAAPASAAFTGAAMQRR"
     gene            4492..6477
                     /locus_tag="VF240_15735"
     CDS             4492..6477
                     /locus_tag="VF240_15735"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEX7176716.1"
                     /translation="MGAVSARLVHLQTSQHEWHLRRARSQQQQTEPAGGERGLILDRA
                     GRELARSVEVESFFAVPVEIEDAQTAAARLASALGIDAVALAERLRRGRETGRDFIWV
                     ARRVEDREAAAVRALGIKGIHSTKEPKRRYPNGALAAHVLGFVGMDEAGLAGVEQFHN
                     VALAGERGRLFVEGDARRKSYESEQTPLRAPQSLVLTIDQTVQYLTERALNAAVARAR
                     AKSGAAVVLDPRTGEILALANAPTFDPNTPGEAKAEVRRNDALQNIYEPGSTFKVVAF
                     SAALEEGLAKPDERIDCQMGTINVAGRIVHDHTAFGTLTMTEALAKSSNVAAIKLGLR
                     VGDARMHDYMTRFGFGAKTGIELPGETAGLLRNYTRWQPSSMGSIAMGQEVGVTPLQM
                     AAAFAALANDGVRVAPHLLRETRNADGTVASRAEPEARRVVSPETARTIRHMLESVTL
                     KGTAKAAQLEGYTAAGKTGTAQKIDPRTRTYSTTKHVASFVGFAPVEAPEVVVAVVID
                     EPVGAYHGGDVAAPVFKEIAENVLPYMSVEPDVEPKQMTPAEGMLARQSRATEAEAAK
                     KEKEAAAAQTESALLPVVAGRDGRGDAVVFAASRERAMLMPDLRGHSVRDAARICAQL
                     GLDLQALGEGRAVRQHPAGGASIEAGQLVRVEFGRSD"
     gene            6845..8314
                     /gene="murF"
                     /locus_tag="VF240_15740"
     CDS             6845..8314
                     /gene="murF"
                     /locus_tag="VF240_15740"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX7176717.1"
                     /translation="MKIRRAAEQMGASARAAVGKLFDNEISGYSIDSRTVGEGELFFA
                     LSPDDYARHGFTGTHFTDAHLFIPDAFARGALAAVALSERVAGDAALEAVAERLLLVE
                     DCIDALQVLAQRELKQWGRPVVGITGSAGKTTTKDLTAHVLAASGRRILRSKKNYNNE
                     LGLPLSVLQMVSEGAAPDDYDVAVLEMGMSTPKELARLVEIAPPDVGVELLVAPVHLE
                     FFGSVEAIAVAKAQLVDGIKPGGTAVLNADDERVAAMRARHTKGPTLTFGIDSRADVM
                     ATEIENAGLGLTRFTLRTPMGEARAELPMPGRHNLMNALAAAATATVFNIEPREIADA
                     LRTAAPSEMRGVVIRFREGFTVVDDSYNSNPRSLLSMAEAVAQGGEGVKRKVIVAGEM
                     LELGPEASAMHREAGRDIGRLGVDVLWGVRDLAREIIAGAQEAGMSEEATRFFDSSEE
                     AAEALVHFVRPGDLVLVKGSRGVQTDKVVKLLKERYEKE"
     gene            8397..9557
                     /gene="mraY"
                     /locus_tag="VF240_15745"
     CDS             8397..9557
                     /gene="mraY"
                     /locus_tag="VF240_15745"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7176718.1"
                     /translation="MIYHIFFEWLFKSLSAGGTESVFVKALNVFQYVTFRTAYATITA
                     LLISLVFGGRVIRALRRLKVGQMIREEGPQTHIAKRGTPTMGGVLIIGSVLISTLLWA
                     RLNSLYIWIILISTTLFAAIGFVDDYQKVAKKQSLGLTGRKKLLGQFLIAVGVWLALY
                     VGTQYGLADYSWRLSVPFFKDTAIRNITLIGPFLYLPFIAVVLLGFSNAVNLTDGLDG
                     LATSVTVVAMSTLTAFTYISGDARWAGYLGLEHQESIGELTVFCGAMVGASLGFLWYN
                     APPAEVFMGDVGSLAIGGAIGTIGVLTKQEFILLVVGGVFVIEALSVMIQVASFKTTK
                     RRVFKMSPLHHHFEILWEQRFGQRTVETKVVFRFLIIAILFALLSLSTLKLR"
     gene            9682..11088
                     /gene="murD"
                     /locus_tag="VF240_15750"
     CDS             9682..11088
                     /gene="murD"
                     /locus_tag="VF240_15750"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7176719.1"
                     /translation="MDLVGKKALVFGAGRSGVAASRFLAARGAVVALNDRKALIDWPN
                     EALHLKADGVGLMAGEVPSWLLDQIELVVVSPGVPANSIPVRYAERAGAEVIGEVELA
                     FRFLKGRVVGITGTNGKTTTTTLVGELLKDAGLNVQVGGNIGTPLVSLADESRDDGWT
                     VVELSSYQLETVKEFRPAVAVVLNVMPDHMDRYESLMDYAAAKHRIFLNQTFEDVAVL
                     NADDELVSSWARGLSAHVVLFSTERELEEGLFLRGRELVCRTPDGERVLMTRDEMQLK
                     GLHNVQNVLAALAAGLACGASPESMRETVVRFAPVEHRLERVAEIGGVTFYNDSKATN
                     VDAAVKAVEALSEEPGRIVLILGGRGKNAPYAPLAPLIEKKGRALVLVGEDAERIEQE
                     LGSYASVERAADMPDAVRRAHAQAQSGDAVLLAPACASFDMFQSYEHRGRVFKEEVKR
                     MESEVGSQESEEKAVAPS"
     gene            11124..12317
                     /gene="ftsW"
                     /locus_tag="VF240_15755"
     CDS             11124..12317
                     /gene="ftsW"
                     /locus_tag="VF240_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX7176720.1"
                     /translation="MARKLQSDEWLFAATVGLALFGVVMVYSASAVVAANQNQNQYHY
                     VVRQGVWTLVGLGVMLWAMQFDYNRLRSPVIVYGLLGLSAVLLVAVFGFSPRNGARRW
                     IQLPGGFTFQPSEVTKLALTLFLARFLERRAGEEGSFWRTFVPCVLVTGVLAGLVIAE
                     PDLGTAMMLGVVCVALLWTAGARVKHMALAAAPALVGVAYLVLFVPFRFKRLVSFLDP
                     WADAQGAGYQVVQSLLAIGSGGADGLGFTQGRQKLFFLPYAHSDFIFAVVGEELGLYG
                     ALGVIALFGIFLWRGMRAALHAPDRFGMLLGVGVVTTIVVQALFNISVTLALLPTKGI
                     PLPFISYGGSSLVPTLFAVGVLLNISQYAGVRGSEMLVNDAQTPLPPRTRTATTACAA
                     SPRHG"
     gene            12327..13445
                     /gene="murG"
                     /locus_tag="VF240_15760"
     CDS             12327..13445
                     /gene="murG"
                     /locus_tag="VF240_15760"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX7176721.1"
                     /translation="MRALIAAGGTGGHIYPGIAVAKEIMRRDANSVVRFVGTAKGLET
                     RLVPQAGFELSLIESAGLVNMSLAARARGAMVLPKSFVAARRLIRSFGPDIVVGAGGY
                     VSGPVLLTAAMMRVPTLVMESNAVPGFTNRVLARFVRAAAVSFEAAAPHFRGKATVTG
                     NPIRREFFDIREKERDSSGGDNTAARFSLLIFGGSQGARAVNEAMMAALPHLAARRDV
                     LRVTHQTGKLDFERVRAAYEAAGWTASADVREYIDDMVSAFAAADLIISRAGATSSFE
                     LMAAGRPAIMVPLPGQLEQRKNAEALQQAGAARMILQNELSGATLAREIDTLVADPER
                     VAEMGRKARSLARGDAAVATVDLIEKLTRGQRSEVRGQ"
     gene            13510..14919
                     /gene="murC"
                     /locus_tag="VF240_15765"
     CDS             13510..14919
                     /gene="murC"
                     /locus_tag="VF240_15765"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7176722.1"
                     /translation="MFRRIQHVHFVGIGGIGMSGIAEVLANMGFRVSGSDMRRSNVTD
                     RLVALGAEFFEGHKAENVSDAHVVVRSTAVRDDNPEVEEARRRSIPVIARAEMLAELM
                     RLKPYNVAVAGSHGKTTTTSMVATVLGHAKMDPTIVVGGIVGSFGSNARLGKSDLMVV
                     EADESDRSFLMLTPTIAVVTNIDREHMDYYRDMTDVRKCFADFVNKVPFYGAAILCLD
                     DPHVQAVIPEVTRRRVTYGLSAQADVSAREVRYDGDFGSRFTVRRAGEPVGQVSLRVP
                     GLHNVYNALAAIAVGFELDVPFETIALGLAEFAGVNRRFQHKGEEAGVLVVDDYGHHP
                     TEIQATLAAAKIGSAGRRMLALFQPHRYTRTKDLMDEFARSFNNADVVLITDIYAASE
                     DPIEGITAETLVEAIRRYGHKNVRHTGSLEDGVAAILEEAREGDMVLTLGAGSISRAG
                     DQLIEKLRERGQRSEARGQ"
     gene            14919..15974
                     /gene="murB"
                     /locus_tag="VF240_15770"
     CDS             14919..15974
                     /gene="murB"
                     /locus_tag="VF240_15770"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX7176723.1"
                     /translation="MTTATMHALAQLTAAVLSEAEVVSEEGNISGTRERDALIEEAAA
                     RTGVRAERGRRFAELTSLRVGGAIDWVLFPETEEQAAGAAGALDDAGVAWRVLGAGSN
                     ILADDREHRYVVLSMREVKGGAVFEGERVSVSAGYSLPRLCVDAARQGLSGIEGLGGI
                     PGTVGGALWMNAGAYGHEIGEVVETVRVARGGRVVEIPGSEVKWNYRHTSFKEGELLL
                     GATLRLRADDPERIRARMEEAKQKRFSTQPHGARSAGCFFKNPPGSGFSTGKMIDEMG
                     MKGERRGGAVVSPVHANFIVTEGEGAQASDALALAEEIRERFRREHGIELEYEVELWG
                     ADREFKDEGGKTKDETD"
     gene            16162..17307
                     /locus_tag="VF240_15775"
     CDS             16162..17307
                     /locus_tag="VF240_15775"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX7176724.1"
                     /translation="MREQVVTPRAGRAAAPRARKETAAAGGQGAGQRPASRKARPASS
                     AAAGKGRQRAFTWRSALAVAPAVGKAVLAVCVGLLVFAGYRAAASASFFQLKTVEVEG
                     AARASRDEIKGMVARAVGADGVWNSDLEELSHGLRELQWVRAAYVQRVLPSGVRVRVV
                     EREPRMVARNAAGRLYWVDEEGVALGPAAPDEGVFFLRGLEEDGDDGARRRNRERMAL
                     VLRLKDDWEKTSLSERVSEVNLHDLRDVRVQLAGGDSEVEVRLGGDHAELFRSALKTL
                     DEKRQTPLGQHVNYIVMIPGKAPVFGYPASLHPSAVSGATATGGEVETKDEAGAAPRG
                     PTPPADPAAARGAERRKQTAPRKKEKDKRDAATGDAPRGAVRERRVG"
     gene            17346..18608
                     /gene="ftsA"
                     /locus_tag="VF240_15780"
     CDS             17346..18608
                     /gene="ftsA"
                     /locus_tag="VF240_15780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX7176725.1"
                     /translation="MAKRATHTVGLDIGTSRVTCIIGEPGEGGFVDIVGIGEADARGL
                     RKGVIVNPEAAVEAIRKAVEDAERMSGLEAEEVSINLAGSHIMGFNGQAIVAVSGRDR
                     EITQDDVRRAIDSACAIQLPAGREIVDRLPQEFIVDDQDGINDPVGMIGARLAVKTHI
                     VTSPVTARQNAINAVNRAGLMVADMVLDQLASAEAALTDEDKEFGAALVDVGAETTGL
                     VIYQRGAVQHTAVFPLGGSHFTNDIAFGLRTPIPEAERIKRSTGCASSTSLSEFERSE
                     LVEVPSVGGRAPRQLSRQILCDILQPRAEEVLMHVADEIRDSGWERQLSSGVVMTGGG
                     ALLDGITEVAEQVFDAPVRVGYPERDRFGGLIEDVQSPAWTAAAGLCLIAQRAQNAEM
                     RAVAARRGTTGGLVAIVSKFRNRFSGIF"
     gene            18752..19918
                     /gene="ftsZ"
                     /locus_tag="VF240_15785"
     CDS             18752..19918
                     /gene="ftsZ"
                     /locus_tag="VF240_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7176726.1"
                     /translation="MTPEGRQPGSGINFFIDEDPPITGARIKVIGVGGGGGNAVNRMI
                     EAGIEGIEFLVANTDLQALKRSRAPIKIQLGGKLTKGLGAGANPEIGRDAALEDTEKI
                     IEALEGADMVFVTTGLGGGTGTGAAPIIASLATELNALTVAVVTKPFHFEGKRRMQQS
                     EIGLRELRECVDTVITIPNERLLHTVDRNMSLQDAFKMADDVLRQAVQGISDLITVPG
                     LINLDFADVKAIMQGMGLALMGAGRASGEHRAMEATQQAISSPLLEEATIEGAKGVLI
                     NITGGYDLTLYEVNEAASIIREAADDDANIIFGAVIDETLRDEMKITVIATGFDKEIA
                     ELGTTTSAAHAVGGAVVPPSRYTPTPTGELPPRPSGPPRSDDLDVPTFIRKKAD"
     gene            20230..20721
                     /locus_tag="VF240_15790"
     CDS             20230..20721
                     /locus_tag="VF240_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176727.1"
                     /translation="MNRKLIRTTLAEVKGRGDTPQHREGGGGGGGREFVSRGGAPSRG
                     GSGGGPPRDRDSGGSSGGGMSAAALGRKKVPPAETNAEIFYYKKQMDQHTEMVIVLLD
                     DEEIEGTIEWYDRNALKINRQGAPNVMLLKHNIKYMYKAEERDGSDSDDADDRGDDGE
                     DDE"
     gene            20809..21882
                     /gene="pdxA"
                     /locus_tag="VF240_15795"
     CDS             20809..21882
                     /gene="pdxA"
                     /locus_tag="VF240_15795"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX7176728.1"
                     /translation="MTDITHTQPHGAPPAEGSARRRPRIAVTMGDPAGIGPEVVLKAV
                     AERAVLDACVPVIVGDAQFLAHTARKLDLQCGYDIVRRGEAFPADADGPVIYHLDNIR
                     GNVEPGVESGSAGRAAAEYIEAAVELCMTGDAAAVSTAPINKRSLFLGGYSFPGHTEF
                     LAHLTGATDCAMAFVASNLRIVLISTHVPLAEAIRMVERERIVRVVRLTERELRRWGI
                     ERPRIAVAALNPHGAEGGLFGMEESAEIVPAVQECQEQHGLDARGPFSADTVFLRASR
                     GEFDAVVSCYHDQAMIPVKCLSFGEAVNVTLGLPFVRTSVDHGTAFDIAGRGVAEHSS
                     MTEAITLAVRLARADGTGQAVEV"
     gene            22041..22451
                     /locus_tag="VF240_15800"
     CDS             22041..22451
                     /locus_tag="VF240_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7176729.1"
                     /translation="MSEQPTQPSRRILVADDDPAILRLVTAIIEKEGCEVVTARDGRE
                     AYKHLQSGGDFIAGVFDVMMPHIQGPELVRYMRSERRLMKIPVMMMTAEQNPKLSSDS
                     FAAGAVVFLPKPFTTSQLQVMLRMLIDKGTQQSS"
     gene            22568..23323
                     /locus_tag="VF240_15805"
     CDS             22568..23323
                     /locus_tag="VF240_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176730.1"
                     /translation="MRQARNNATRFDASGSRTTHTGLLLLALALLCVVAAATAGAQSG
                     RRKTAPISPVPTPTPAAPEAEGESESESRSRRQVGGPAQLSLVVYRLDNAFPYADYNV
                     EDVLMGGLMDRLTRSKDLSASRGGKRISRKEAQEIAKSESQSHVVLVELEEDIGASGG
                     RSGGAGQTDTRTLAVKTYVYEMGTGTLKFVDQTSQRPYRGQTTSIGGIRLPLPTTRTR
                     MERFPGELQLEQAARDAADRLMHRFKLRPPPDN"
     gene            23455..>23768
                     /locus_tag="VF240_15810"
     CDS             23455..>23768
                     /locus_tag="VF240_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176731.1"
                     /translation="MNSFNPPLPLRVLRGSAVNHPPTSFQVKFHAGRPLTLLLCAMLL
                     VIPSASFATEKKPKKAIKDSDARKVIAATPGFDLRTGRVRVKDVSLAGATPVVVNAEV
                     EIA"
BASE COUNT         4195 a         7655 c         8137 g         3781 t
ORIGIN      
        1 ttgtagtccg aacgcagctc ggcgtactcg cccgtcgccg ccgagccgta gagcacgacc
       61 gacgcgagat tctcgccgtg cgtcgcccgc aagtcgccga tcagatggtt gaatgcttcc
      121 tgcccaatcc tgttcgccat agtctttacc agtctccgcc ggggccgccg ccgccgccga
      181 agtcgccgcc gccgccgaat ccgccgaacc cgccgccgcc gccccagccc gacgacgatc
      241 caccgccacc accgcctccg ccccaaccgc tcgaaccccg cccgccgaga ttgctaagca
      301 cctgaccgag gataagcgcg ttcagaagac cgccacctcc gccccagcct ccgccacgtc
      361 cgccccgccc gccaccacca ccgcgccggc cgatggaaga gagcaccagc aggaggacga
      421 agccgatgag gcagcacgcg cccagaccga gactgtttct gccgacgggt acgggccgcc
      481 cgcgcgtcgg gccttgggcg cgggggcgat agacgtaact ctggtcgatg ccctcgatgc
      541 tgaagccgcg tttgtccgcc agcgtcgcta ccagagtcag caccgcctcc gacaatcccc
      601 gcccgtactc gcccgcgcgg aacgccgccg gcagtttctc gcgcaggatc gtcgtgacga
      661 gaccgtccgg caaatcccct tcgagatgcc ggctcacctg cggctgaaac tttctgtcat
      721 cgacggcgac cacgagcagc agcgcgttct tgtcgccctc cttcgagccg aggccccagc
      781 agcgcgccag cgccagcgag tagtcgaaga tcgcccgctc gcccgtcgtc tttacggtga
      841 cgacggcgaa ctcgacctcg gcccgcccct tgagatttgt cagcaccgcc tccatccgct
      901 ccttcgacgc cgcgtcgatg acgcccgcgt aatcgttgac gtagccggtg ggcgcgggga
      961 ggggacagtt ctgagacact tgcgcgtgcg ccgcgacggg gcacaggagc agacagagca
     1021 gcgcgacgaa agcgccgtgc gagagtagga gccggagttg catcggttgt tgtgatgata
     1081 gcagcaagca ggcggaaaga aaaaagacgc ggggggcgcc cgcgcgtctg cgccggagac
     1141 gcgcgcgggc gcgtgtcttc gcttcagccc tcgatgagca ggtggaagag gtagaacagg
     1201 gcgacgccga cgaagacgac gagcgagacg agcgggttct tctcttcgag gtggttgacc
     1261 tccgggatga gatcgctcgc cgccacgtag agcgtgacgc ccgccgagaa gggaagcgcg
     1321 tagagaaccg cgtccgtcac gcgcgactgg agcagcgcga cggtgagcac gccggcgagc
     1381 gtcgccgcgc cgatgacaag cgtcgagagg aaggcgctac gcgttgagcg cccggaggcc
     1441 agcacgatgg aggcggccgt gaacccctcg ggaaccttgt gcagcagaac ggcgaggaag
     1501 acgagcagcc cgagtttgtg gccgaccaga aaggcggcgg ccacggagac gccgtcgaaa
     1561 aacgtgtgga tggtgaggcc gccgatggcc gtgtaggcgg ccgacgggcg gatgagagcc
     1621 tccgggtgtg tctccgcccc aaagtggaag tgcggcgcaa tcgtgtgctc gacaaagtgg
     1681 acgagaagat aaccagccag cagaagcacc atcgcactca aaacgccgcc ttcacccctg
     1741 ctgccgcccg cacccgacgc gctccagagt tccaccacat tcggcatgat ctcgatgaaa
     1801 atggccgcca gcatgaagcc ggctccgagg gccacgaggt atttgagcgt gcggaggctg
     1861 cggacggtcg cgccggggcg tgccagaatc agcccgccca ggacgttccc cagcgccacg
     1921 agaaggccga agaagaggat tttgccgaag ttcatctagg ctctagagga cgccccgtcc
     1981 cgtaaatgga tgcttaagca cagcggtcac tatagcgcaa gccgcacgac tttgctacgc
     2041 cactcttaag cacaggccgc accatatcgt gcttaaaata atcaatttaa catcattttt
     2101 ctgcttgaca gccttcgttg gacaagcgta ctatgcgcgg caggtgggaa aaagtggcca
     2161 aaagtgggcc tttcagagta gcttgtggcc attcattaaa gtatcgctct aacttttaag
     2221 cagccctcgc cccacatccg gccacaaccc ttatgctgag aggaaattac acggcccgga
     2281 ttgatacgaa gggacgtttg aaggtgccga cagcgtttcg gcgatatata gaggacaagt
     2341 acggggcgga gttttacgtc acaagcctga gcggggattg cgcgcgcatc tacccgctgc
     2401 ccgagtggga gacgattgaa cagcggttgg cgcttctgcc ctcgatggac ccggcgaggc
     2461 gcaagttcct tgacaggacg aactactacg gacaacaggc ggcgatggac gcgcagggac
     2521 gcgtgctcat ccacccgctg ctacgcaaaa gcgcgggcgt cgtgggcgac gtagcggtac
     2581 tcggatattt gacatacctg gaggtttggg agctggagag gtttgaacga caacttctct
     2641 ccgaccccta cagggaagag gacgaggcgg caatcgctcg actcggaatc tgagagcagt
     2701 ggcaggtgag gggtgacaag ttgagaggga aagttttttc ttcgactcga tcactcgtca
     2761 ctcgtcactt gccactgctc tccgatggca cggggcggtg gtgatggcgg agacaggagg
     2821 ggggcctgtc gcgccccatc gccccgtgtt gctcactgag gtggtcgcgc tgctcgcacc
     2881 cgagcggggc ggcctcttca tcgacgccac actcggactc ggcgggcaca gcgaggcgct
     2941 cctcgaagcg tcgccggacg tgcgggtgct cggcctcgac cgcgaccgcg aggcgctacg
     3001 tctggccacg gaacggctcg cgcgtttcgg ctcgcgcttc cgcgccgcgc acgcaaactt
     3061 caaagaggtc gcccgcgtga tgagtgatgc gggcgagcgc gacgcggcgg gtctgctggc
     3121 cgacctcggc gtctcatcgc ttcagtttga cacggccgga cgcggcttca gcttccgcca
     3181 cgacgcgccg ctcgacatgc ggatggacgc cgaggggcag gaggagacgg ccgccgaact
     3241 cctcgcccaa cttcccgagg aggaaatcgc gcgcgtcatc tacgagtacg gcgaggagcg
     3301 tcggtcgcgc cggatagcgc ggtggattgt tgagaggcga gagcgcgggg cgccgataca
     3361 gacgacgacg gagctggccg agttggtggc gcgcgccgtc ggccacaagc gcggcgagcg
     3421 gattcacccg gcgacgcgaa ccttccaggc gctgcgcatc gccgtcaacc gggagctgga
     3481 aggtctcggc gagttcattg aacgggcagt cgatctttta caaccggcgg gccgcttggc
     3541 cgtcatcagc ttccactcgc ttgaggatcg cgtcgtgaag cgcacgctcc agcgcctcgc
     3601 cggcaagtgc gaatgcgacc cgcgcttccc ggtctgtact tgcggcgcgc ggcgcgcggt
     3661 cgagttgttg acgaggcggc cggtcgtgcc gaccgcggag gaagcggaag ataacccgcg
     3721 cgcgcggagt gccaagctgc gcgcctgcca gaagctctaa tcaagagacc gagggaaccc
     3781 aaccctcacc cgacacgcaa gtctgccgac gcgatcaact ccctgttttt tggtaaagcg
     3841 ttccgaggcc cgataacaaa gatgagaaga gtgccccagc aacacgcaaa ctcctacgtc
     3901 cgccgcgagc gcgacccgcg caccctcgca cggcaggcgt tgcttctggc gagctgtgtc
     3961 gtgctcgccg ccggtttcgt gctcgcggcg cggcagaaga tcgccgccgt gcagtacggc
     4021 taccgcagcg aggagttgcg gcgcgagcgc gagcgacttc tggaggagca gcatcggctg
     4081 cttgtcgccg tcgaggaaag ctcctcgccc gtgcatctcg aacgagcggc gcggacgctc
     4141 ggcttacaga cggcgcgcgc ggcgcagatc ggggtgagcg agaccgtgac gcccgaacgt
     4201 ggcggcgtgg aggacatgtc cggggccgcg cggacggggg ctgagggcgc ggactcgaag
     4261 ccgcgcagga agtcgggcgg tgtgacggcg gcgcccgcgt ccgcggcatt cacgggtgcg
     4321 gcaatgcagc gccgttgaaa agggagggcg aacgaaggcg cgcttccgaa gagcttgatg
     4381 agagggccgg ttctgctaat gcccaggagc cactccgcgg tgcaaaagag ggtgcgacct
     4441 gacgtctcac ggcgacgtgc gatagcggtc gccctttttc tttgcgcgtg gatgggggcg
     4501 gtctcggcgc ggctcgtcca cctgcaaacg tcgcagcacg agtggcacct gcggcgcgct
     4561 cgctcgcagc agcagcagac cgagccggcg ggcggcgagc gcggcctcat cctcgaccgc
     4621 gcgggccgcg aactggcgcg cagcgtcgag gtcgaatcat tcttcgccgt gcccgtcgag
     4681 atcgaagacg cgcagacggc ggccgcgcgc ctcgcgtccg cgctcgggat agacgccgtc
     4741 gcgctcgccg agcgtctgcg acgcgggcgg gagacggggc gcgacttcat ctgggtcgcg
     4801 cggcgggtgg aagaccgcga ggccgcggcc gtccgcgcgc tcggcatcaa gggcatccat
     4861 tcgacgaagg agccgaagcg gcgctacccg aacggcgcgc tcgccgcgca cgtgctcggc
     4921 ttcgtcggca tggacgaggc gggcctggcc ggcgtcgagc agtttcacaa cgtcgcgctc
     4981 gcgggcgagc gtgggcgcct cttcgtcgag ggcgacgcgc gccgcaagtc ttacgagagc
     5041 gagcagacgc ctttgcgagc gccgcagtcg cttgtgctga cgattgatca gaccgtgcaa
     5101 tatctgacgg aacgggcgct caacgcggcg gtcgcgcgcg cacgcgccaa gtcgggcgcg
     5161 gccgtcgtcc tcgaccctcg cacgggtgag attctggcgc tcgccaacgc cccgacgttc
     5221 gaccccaaca cgccgggcga ggcgaaggcc gaggtgcggc gcaacgacgc gcttcagaac
     5281 atctacgaac ccggctcgac cttcaaggtc gtcgccttct cggccgctct ggaggaaggt
     5341 ctcgccaagc ccgacgagcg catcgactgc cagatgggca cgatcaacgt cgcgggccgc
     5401 atcgtccacg accacacggc cttcggcacg ctgacgatga ccgaggcgct ggcgaagtcg
     5461 agcaacgtcg ctgccatcaa gttgggcctg cgggtcggcg acgcgcgcat gcacgactac
     5521 atgacgcgct tcggcttcgg cgcgaagacc ggcatcgagc tgccgggcga gaccgccggg
     5581 ttgctgagaa actacacgcg ctggcagccg agttcgatgg gttcaatcgc gatggggcag
     5641 gaagtcggcg tcacgccctt gcagatggcc gcggcgtttg cggcgctcgc caacgacggc
     5701 gtccgcgtcg cgccgcacct gttgcgcgag acgcgcaacg ccgacggcac cgtggcctcg
     5761 cgcgccgagc cggaggcgcg acgtgtcgtc agccccgaga cggcgcgcac catccggcac
     5821 atgttggaga gcgtgacgct gaagggcacg gcgaaggcgg cgcagctcga aggctacacg
     5881 gccgccggca agaccggcac ggcgcagaag atcgacccgc gcacgcgcac ctattcgacc
     5941 accaagcacg tggcctcgtt cgtcggcttc gcgccggtcg aggcgccgga ggtggtcgtc
     6001 gccgtcgtca tcgacgaacc cgtgggcgca taccacggcg gcgacgtggc cgcgcccgtc
     6061 ttcaaggaga ttgccgaaaa cgtcctgccc tacatgagcg tcgagccgga cgtggagccg
     6121 aagcagatga cgcccgccga ggggatgctc gctcgccagt cccgcgcgac ggaagcggag
     6181 gcggctaaga aggagaaaga agcggcagca gcacagaccg agtcggcgct gctgccggtg
     6241 gtcgccgggc gcgacgggcg cggcgacgcg gtcgtcttcg ccgcgtcgcg cgagcgcgcg
     6301 atgctgatgc ccgacctgcg cggccacagc gtgcgcgacg cggcgcgcat ctgcgcgcaa
     6361 ctcgggcttg accttcaggc gctcggcgag gggcgcgccg tcagacagca cccggcgggc
     6421 ggcgcgagca tcgaggccgg acagttggtg cgcgtcgagt tcgggcgcag cgattgagaa
     6481 ggattgagtc attgactcaa tgaaaaacag taccgcgagc ggtagcgagc gggtctgcct
     6541 ccgacgagag gcccgcaaca cgcactggcg cccgctcgct accgctcgcg gtactgtttt
     6601 aaatttcaac cccggaattt cgagatgcac aatcgccagg cgacgcagat taacgaacac
     6661 acggacggcg ggcatgaaga gatgagcaga agaatcgccg gatgccgcga gcgtttgcgc
     6721 cgtaatcgcg cgacccacac aatagctcag cagtgcgccg cgcccgattc cgatgggcgc
     6781 gcgacggggg aagacacagg ctccagcgcc cccgcgcaac gggcgcacag gaaagagtct
     6841 gagcgtgaag attagacgag cggccgaaca gatgggggcc tccgcgcggg cggcggtcgg
     6901 gaagttgttt gacaatgaaa taagtggata ctcaatcgac tcgcgcaccg tgggggaggg
     6961 tgaactcttc ttcgccctct cccccgacga ctacgcgcgc cacggcttca cgggcacgca
     7021 cttcacggac gcgcacctgt tcatccccga cgccttcgcg cgcggcgcgc tggcggccgt
     7081 ggccctctcg gagcgcgtcg cgggggacgc cgctctcgaa gctgtggctg aacgccttct
     7141 gctcgtcgag gattgcatcg acgctctaca ggtgctcgcg cagcgcgaac tgaagcagtg
     7201 gggccgaccc gttgtcggca tcacggggag cgcgggcaag acgacgacga aggacttgac
     7261 ggcacatgtg ctcgccgcct cgggccggag gattttgagg agcaagaaga actacaacaa
     7321 cgaacttggc ctgccgctct ccgtcctcca gatggtctcg gagggcgccg cgcccgacga
     7381 ctacgacgtg gccgtgctgg agatgggaat gtccacgccg aaggagctgg cgcggctcgt
     7441 cgagatcgcg ccgccggacg tgggcgtgga gcttctggtc gcgcccgtcc acctggagtt
     7501 cttcggctcg gtcgaggcga tagcggtggc gaaggcgcaa ctcgtggacg gcatcaagcc
     7561 gggtgggacg gccgtcttaa acgccgacga cgagcgcgtc gccgcgatgc gcgcgcgcca
     7621 cacgaagggg ccgacgctca cgttcggcat cgacagccgc gcggacgtga tggcgaccga
     7681 gatcgagaac gccggcctcg gtctcacgcg cttcaccctg cggacgccga tgggcgaagc
     7741 gcgggcggaa ctgccgatgc cggggcgaca caatttgatg aacgcactcg cggccgccgc
     7801 cacggccacc gtttttaaca tcgagccgcg cgagatcgcc gacgcgcttc gcacggcggc
     7861 gccctcggag atgcgcggcg tggtcatccg cttccgcgaa gggttcaccg tggtggacga
     7921 ctcttacaac tcgaacccgc gctcgctgct ttcgatggcc gaggccgtgg cgcagggcgg
     7981 cgagggggtg aagcggaagg tgatcgtcgc cggcgagatg ctcgaactcg gccccgaggc
     8041 gtccgccatg caccgcgagg cgggccgcga catcggccgg ctcggcgtgg acgtgctctg
     8101 gggcgtgcgc gacctcgcgc gcgagattat agccggcgcg caagaagccg gaatgagcga
     8161 agaggcgacg cgcttcttcg actcgtccga ggaggcggct gaggcgttag ttcacttcgt
     8221 ccggcccggc gacctcgtcc tcgtcaaagg ctcgcgcggc gtgcagacgg acaaggtcgt
     8281 gaagctgttg aaggagaggt acgagaagga gtgatgagtg atgagtgata agtgataaga
     8341 aaatcctgat tcaatttctt atcacttatc actcatcact catcactgcg aagccgatga
     8401 tctaccacat cttcttcgag tggctcttca aatccctctc ggcggggggg acggagtcgg
     8461 ttttcgtcaa ggcgctgaac gtcttccagt acgtgacgtt ccgcacggcc tacgcgacga
     8521 tcacggcgct gctgatctcg ctcgtcttcg gcgggcgcgt catccgggcg ctcaggcgtc
     8581 tgaaggtcgg acagatgatt cgcgaggagg ggccgcagac gcacatcgcc aagcgcggca
     8641 ccccgacgat gggcggcgtg ctcatcatcg gctccgtgct catctcgacg cttttgtggg
     8701 cgcgtctcaa cagtctctac atctggatca tcctgatttc gacgacgctc tttgcggcca
     8761 tcggcttcgt ggacgactac cagaaggtcg ccaagaagca gagcctcggc ctgacggggc
     8821 gcaagaaact gctcgggcaa tttttgatag ccgtcggcgt ctggctcgcg ctctacgtcg
     8881 gcacgcagta cgggctggcc gactactcgt ggcgattgag cgtgccgttc ttcaaggaca
     8941 cggccatcag gaacatcacc ctgatcggcc cgttccttta tctgccgttc atcgcggtcg
     9001 tgctcctcgg attctcgaac gcggtgaacc tgacggacgg cctcgacggg ctggcgacga
     9061 gcgttacggt ggtggcgatg tccacgctga cggcgttcac ctatatcagc ggcgacgcgc
     9121 gttgggccgg ctacctcggt ctcgaacatc aggagagcat cggcgagctg acggtctttt
     9181 gcggggcgat ggttggggcg agcctcggct ttctgtggta caacgcgccg cccgccgagg
     9241 tcttcatggg cgacgtgggg agcctcgcca tcggcggggc catcggcacg ataggagttt
     9301 tgacgaagca ggagttcatc ctgctggtgg tcggcggcgt gttcgtcatc gaggcgctgt
     9361 cggtgatgat tcaggtggct tcgttcaaga cgacgaagcg gcgcgtcttc aagatgtcgc
     9421 cgctccatca ccactttgaa attttatggg agcagcgttt cggacagagg acggtggaga
     9481 cgaaagtcgt cttccgcttc ctcatcatcg cgatcctgtt cgcgctcctg agtctttcga
     9541 ccctgaagct gcgctgagga gagcagtttg aaactaacgt ggatggacag gatcggccgg
     9601 gtacacagaa atagacagcc gacccttctt tcctcaaatt ccgcgtcccc cgtcctcccg
     9661 tgttcgttct gtgattcgga gatggacctg gtcggcaaaa aagctctggt cttcggtgcg
     9721 ggccgcagcg gcgtggcggc ttcacgcttc ctcgccgcgc gcggcgccgt cgtcgccttg
     9781 aacgaccgca aggcgctcat cgactggccg aacgaggcgc tgcacctcaa agccgacggc
     9841 gtcggcctga tggcgggcga ggtgccttcg tggctgttgg atcagatcga gctggttgtc
     9901 gtcagccccg gcgtgccggc caacagcatc ccggtgcgct acgccgagcg cgcgggcgcg
     9961 gaggtgatcg gcgaggtcga gctggcgttt cggtttttga agggccgcgt ggtcggcatc
    10021 acgggcacca acggcaagac gacgacgacg acgctcgtcg gcgagctttt gaaggacgcc
    10081 ggactaaacg tgcaggtcgg cggcaacatc gggacgccgc tcgtctcgct cgccgacgag
    10141 tcgcgcgacg acggctggac ggtggtcgag ctttcgagct atcaactcga aaccgtcaaa
    10201 gagttccggc ccgcggtcgc cgtcgtcttg aacgtcatgc ccgaccacat ggatcgctac
    10261 gagagtttga tggactacgc ggcggccaag caccgcatct tcctcaacca gacgtttgaa
    10321 gacgtggcgg tcttgaacgc ggacgacgaa ctcgtctcct cgtgggcgcg agggctgagc
    10381 gcgcacgtcg tcctcttcag caccgagcgc gagctggaag aggggctgtt cctccggggc
    10441 cgtgaactcg tctgccgcac gcccgacggc gaacgcgttt tgatgacgcg cgacgagatg
    10501 cagctcaagg gtctgcacaa cgtgcagaac gtgctggcgg cgctcgcggc cgggttggcc
    10561 tgcggcgcca gccctgagtc gatgcgtgag acggtcgtgc gcttcgcgcc ggtcgagcac
    10621 cggctggagc gtgtcgcgga gattggcggc gtcaccttct acaacgactc gaaggcgacg
    10681 aacgtggatg cggccgtcaa agccgtcgag gcgctctccg aagagccggg gcgcatcgtc
    10741 ctcatcctcg gcgggcgcgg caagaacgcg ccctacgcgc cgctcgcgcc gctgattgaa
    10801 aagaagggcc gcgccctcgt cttagtcggt gaggacgccg aacgcatcga gcaggagctt
    10861 ggttcttacg cgagcgtcga gcgcgccgcc gacatgcccg acgccgtccg ccgcgcgcat
    10921 gctcaggccc aaagcggcga cgccgtgctg ctcgccccgg cctgcgccag cttcgacatg
    10981 ttccagagct acgagcaccg tgggcgcgtg ttcaaggaag aggtaaagag gatggagtca
    11041 gaagtcggga gtcaggagtc agaagagaaa gcggtcgcgc cttcttaatt ctgactcctg
    11101 actcctgact tcccgaagtt gttatggccc gcaagctaca gagtgacgaa tggctgttcg
    11161 cggcgaccgt ggggctggcg ctcttcggcg tggtgatggt ttacagcgcg tcggcggtgg
    11221 tggccgcgaa tcagaatcag aaccagtatc actacgtcgt gcggcagggc gtgtggacgc
    11281 tcgtgggcct gggcgtgatg ctgtgggcga tgcagttcga ctacaaccgt ctgcgcagcc
    11341 ccgtcatcgt ctacgggttg ctcgggctgt cggccgtgct gctcgtcgcg gtcttcggct
    11401 tctcgccgag aaacggcgca cggcgttgga tacaactgcc gggaggcttc accttccagc
    11461 cgtcggaggt gacgaagctc gcgctgacgc tcttcctcgc gcgctttctc gaaagacgcg
    11521 cgggcgagga aggctccttc tggcggacgt tcgtgccgtg cgttttggtg acgggcgtgc
    11581 tggcgggcct cgttatcgcg gagccggatt tggggacggc gatgatgctg ggcgtcgtct
    11641 gcgtcgcgct gctgtggacg gccggggcgc gcgtcaaaca catggcgctg gcggcggccc
    11701 cggcgctcgt cggcgtcgcc tacctcgtcc tgttcgtgcc gtttcggttc aagcggctcg
    11761 tctcttttct cgacccgtgg gccgacgcgc agggcgcggg ctatcaggtc gtccagtcgc
    11821 tactcgccat cggctcgggc ggggccgacg gcctcgggtt cacgcagggg cgacagaaac
    11881 ttttcttcct gccctacgcc cactccgact tcatcttcgc ggtggtcggc gaggaactcg
    11941 ggctttacgg cgcgctcggc gtgatcgcgc tcttcggcat ctttttgtgg cgcgggatgc
    12001 gcgcggccct acacgcgccc gaccgtttcg ggatgctgct cggcgtcggc gtggtgacga
    12061 cgattgtcgt gcaggcgctc tttaacatca gcgtgacgct ggcgctgctg ccgacaaagg
    12121 gcatcccgct gccgttcatc tcctacggcg gctcgtcgct cgtcccgacg ctcttcgccg
    12181 tcggcgtgct cttgaacatc tcgcagtacg cgggcgtgcg cggcagcgag atgttagtga
    12241 atgacgcgca gacgccgctg ccgccccgaa cgcgaaccgc gacgaccgcg tgcgctgcgt
    12301 cgccgcggca cgggtgagcc ggggtgttgc gcgcactgat tgccgccggc gggacgggcg
    12361 ggcacatcta tcccggtatc gcggtggcga aagaaatcat gcgccgcgac gcgaactcgg
    12421 tcgtgagatt tgtcggcacg gccaaaggat tggagacgcg gctcgtgccg caggcgggtt
    12481 ttgaactttc gctcattgaa tcggcggggc tggtgaacat gtcgctcgcg gcgcgggcgc
    12541 gtggggcgat ggttctgccg aaaagtttcg tggcggcgcg gcggctgata cgcagcttcg
    12601 gccccgacat cgtcgtcggc gcgggcggct acgtctccgg gccggtgctt ttgacggcgg
    12661 ccatgatgcg cgtgccgacg ctcgtcatgg agtcgaacgc ggtgccgggg ttcacgaacc
    12721 gcgtgctggc gcgtttcgtc cgcgcggcgg ccgtctcgtt cgaggcggcg gccccccact
    12781 ttcgcggcaa ggccacggtt accggcaacc ccatccggcg cgagttcttt gacattcgag
    12841 agaaggaacg cgacagcagc ggcggcgaca acacggcggc gcgtttctca ctcctcatct
    12901 tcggcggctc gcagggcgcg cgcgccgtca atgaggcgat gatggcggcg ctgccgcacc
    12961 tcgcggcgcg acgcgacgtg ctccgcgtca cacaccagac cggcaagctc gacttcgagc
    13021 gcgtgcgcgc cgcatacgag gccgccggct ggactgcttc ggcggacgtg cgcgagtaca
    13081 tcgacgacat ggtctcggcc ttcgccgcgg ccgacctcat catttcgcgt gccggggcca
    13141 cttcgagctt cgagctgatg gccgcgggac ggcccgcgat catggtgcct cttccggggc
    13201 agctcgaaca acgcaagaac gccgaggcgc tccaacaggc cggcgccgcc cgcatgatct
    13261 tgcagaacga actctccggc gcgacgttag cgcgcgagat cgacacgctt gtcgccgacc
    13321 ccgagcgcgt cgccgagatg ggccgcaagg cacgcagcct tgcacgcggc gacgccgcgg
    13381 tggcgaccgt ggatttgatt gaaaaactga caagaggtca gaggtcagag gtcagaggtc
    13441 agtgaaagca ttaaaacact gtcttctact aacctctaac ctctaacctc tgacctcttt
    13501 ggcttgaata tgttccgtcg catccaacac gttcacttcg tcggcatcgg cggcatcggc
    13561 atgtcaggca tcgccgaggt gctggcgaac atgggcttcc gcgtgtccgg ctctgacatg
    13621 cggcgctcaa acgtgacgga caggctcgtg gcgttgggcg cggagttctt tgaaggtcac
    13681 aaagcggaga acgtctcgga cgcgcacgtg gtcgtgcgct cgacggccgt gcgcgacgac
    13741 aacccggagg tggaggaggc gcgccgtcgt tcgatacccg tcatcgcgcg cgccgagatg
    13801 ctggccgagt tgatgcggct caagccgtac aacgtcgccg tcgccggctc gcacgggaag
    13861 acgacgacca cttcgatggt ggccaccgtg ctcggccacg ccaagatgga cccgaccatc
    13921 gttgtcggcg gcatcgtcgg ctcgttcggg tcgaacgcgc gcctcggcaa gagcgacctg
    13981 atggtggtcg aggccgatga gagcgaccgc tcgtttctga tgctgacgcc gaccatcgcc
    14041 gtcgtcacca acatcgaccg cgagcacatg gactactacc gcgacatgac ggacgtgcgt
    14101 aaatgtttcg ccgacttcgt caacaaggtg ccgttctacg gcgccgcgat tctctgtctc
    14161 gacgacccgc acgtgcaggc cgtcatcccc gaagtgacgc gccgccgcgt gacctatgga
    14221 ctgtcggcgc aggccgacgt ttccgcgcgc gaggtgcgct acgacggcga cttcggctcg
    14281 cgtttcacgg tgcggcgcgc gggcgaaccc gtcgggcagg tgagtctgcg agtgccgggt
    14341 ttgcacaacg tctacaacgc gctggcggcc atcgccgtcg gcttcgagtt ggacgtgccg
    14401 ttcgagacca tcgctttggg cttggcggag ttcgccggcg tcaaccgacg cttccagcac
    14461 aagggcgagg aggcaggcgt ccttgtggtg gacgactacg gacaccaccc gaccgagata
    14521 caggcgacgc ttgcggccgc caagatcggt tcggccgggc ggaggatgct ggcgctcttc
    14581 cagccgcacc gctacacgcg gacgaaggac ttgatggacg agttcgcgcg ctctttcaac
    14641 aacgccgacg tggtgctcat caccgacatc tacgcggcga gcgaagaccc tatcgagggc
    14701 atcactgccg aaactctggt ggaggccatc aggcgctacg gccacaagaa cgtgcgccac
    14761 acgggctcac tcgaagacgg agtggccgcc atactcgaag aggcgcgtga aggcgatatg
    14821 gtgctcacgc ttggcgcggg cagcatcagc cgcgcgggcg atcagttgat cgagaagctg
    14881 cgggaaagag gtcagaggtc ggaggccaga ggccagtgat gacgacggcg acgatgcacg
    14941 cgctggctca gttgaccgca gcagttttga gcgaagcaga agttgtgagt gaagagggaa
    15001 acatctccgg cacgcgggaa cgcgacgcgc tcatcgagga ggccgccgcg cgaacgggcg
    15061 tgcgtgcgga gcgtggacgg cgcttcgccg agttgacttc gctgcgcgtc ggcggggcga
    15121 ttgactgggt gctgttccct gagacggagg agcaggcggc cggcgcggct ggcgcactcg
    15181 acgacgctgg cgtggcgtgg cgcgtgctcg gagccggcag caacatcctc gccgacgacc
    15241 gcgagcatcg ctacgtcgtt ctttcgatgc gcgaggtgaa gggcggggcc gtgttcgagg
    15301 gcgagcgcgt ctcggtgagc gcgggctact cgctgccgcg tctgtgtgtg gacgcggcgc
    15361 gtcaggggct gtcggggatt gaaggcttgg gcggcatccc cggcaccgta ggcggcgcgc
    15421 tctggatgaa cgcgggcgcc tacggccacg agatcggcga ggtggtcgag acggtgagag
    15481 tcgcgcgcgg gggccgggtc gtcgaaattc ccggaagtga agtgaagtgg aactatcgtc
    15541 acacctcctt caaggaaggc gagctgttgc tgggcgcgac gctcaggctg cgcgcggacg
    15601 acccggagcg gatacgcgcg cggatggagg aggccaaaca gaaacgtttc agcacgcagc
    15661 cgcacggcgc acgctcggcc ggctgtttct tcaagaatcc gcccggctcg ggctttagca
    15721 cggggaagat gatcgacgag atgggaatga aaggcgagcg tcgcggcggc gccgtggtct
    15781 cgcccgtcca cgctaacttc atcgtcaccg agggcgaggg cgcgcaagcc tccgacgccc
    15841 tcgcgctcgc cgaggagata cgcgaacgct tccgccgcga gcacggtatc gaactcgaat
    15901 acgaagtcga gctgtggggc gccgacaggg agtttaagga cgagggtgga aagacgaagg
    15961 atgaaacgga ttgagtcatc gggtcatcaa ctcaatgagg agtggccgtc acgcacagta
    16021 ccgcgagcgg tagcgagcgg gtctgacgcg cgcgaggcga gccgcttatc aaggccgcac
    16081 ccgctcgcta ccgctcgcgg tactgattga gccgttgggc cgatgagtaa atgaatcaat
    16141 cctcatgggg gaagtaagaa gttgagagag caagtcgtca caccgagagc aggtcgcgcc
    16201 gccgcgccgc gggccaggaa ggaaactgcc gccgcgggcg ggcagggcgc cgggcagcgt
    16261 cccgcgtcgc gcaaagcccg tcccgcttcg tccgccgcgg cgggcaaggg ccggcagcgc
    16321 gccttcacgt ggcggagcgc actcgccgtc gcgccggcgg tcgggaaggc cgtgcttgca
    16381 gtgtgcgtcg ggctgctcgt cttcgcgggc taccgggcgg cggcttcggc gtcgttcttc
    16441 cagttgaaga cggtcgaggt cgagggcgcg gcgcgcgcct cgcgcgatga gatcaagggc
    16501 atggtcgcgc gcgcggtcgg cgctgacggc gtttggaact ccgacctcga agagttgagc
    16561 cacggcctgc gagagttgca gtgggtgcgc gccgcatacg tgcagcgcgt gctgccctcg
    16621 ggcgtgcgcg tgcgcgtcgt cgagcgcgag ccgcgcatgg tcgcgcgcaa cgcggcgggt
    16681 cgcctgtatt gggtggacga agagggggtc gcgctcgggc cggccgcgcc cgacgaaggc
    16741 gtcttcttcc tccgcggtct tgaagaagac ggggacgacg gcgcgcgccg gcgcaaccgc
    16801 gagcgcatgg cgctggtgct gcgtctgaag gacgattggg agaagacttc cttgagcgag
    16861 cgcgtcagtg aagtcaacct ccacgacctg cgcgacgtgc gcgtgcagct cgcgggcggt
    16921 gattccgagg tcgaagtccg gctcggcggc gaccacgccg agttgttccg cagcgccctc
    16981 aagacgctcg acgagaagcg gcagacgccg ctcggccagc acgtcaatta catcgtgatg
    17041 attccgggca aggccccggt tttcggatac ccggcgagcc tgcacccttc ggccgtgagc
    17101 ggcgcaacgg ccacgggcgg cgaagtcgag acgaaggacg aagcgggcgc tgcgcctcgc
    17161 ggcccgacgc cgccggccga cccggccgca gcgcgaggcg cggagcgcag gaagcagacc
    17221 gcgccgcgga agaaagaaaa ggacaagcgg gacgcggcga ccggcgacgc accacgcggg
    17281 gcggttcgcg agcgacgggt cgggtgagcc gacagagaac actctctcaa aggatgagac
    17341 cagcaatggc aaaacgcgct acgcacaccg tcggcctcga catcgggacg agtcgcgtga
    17401 cgtgcatcat cggcgagccg ggcgagggcg gtttcgtgga catcgtcggc atcggcgagg
    17461 cggacgcgcg cggactccgt aagggcgtca tcgtcaaccc cgaggcggcc gtcgaggcga
    17521 tccgcaaggc ggtcgaggac gccgagcgga tgagcgggct ggaggccgag gaggtttcca
    17581 tcaacctcgc cggctcgcac atcatgggct tcaacggcca ggccatcgtc gccgtctcgg
    17641 gccgcgaccg cgagatcacg caggacgacg tgcgccgggc gattgattcc gcgtgcgcca
    17701 tacaactccc cgccggccgc gagatcgttg accgtctccc gcaggagttc atcgtcgatg
    17761 atcaggacgg catcaacgac ccggtcggca tgatcggcgc gcgtctcgcc gtcaagacac
    17821 acatcgtcac cagccccgtc accgcgcgcc agaacgccat caacgccgtc aaccgcgccg
    17881 gcctcatggt cgccgacatg gtgctcgacc aactcgcctc ggccgaggcc gcgctcaccg
    17941 acgaagacaa agagttcggc gcggcgctcg tggacgtggg cgcggagact acgggcctcg
    18001 tcatctacca gcgcggggcc gtccagcaca cggccgtctt ccctctgggc ggctcccact
    18061 tcactaacga catcgccttc ggactgcgca cgcccatccc cgaggccgag cgcatcaagc
    18121 gttcgacggg ttgcgcgtcg agcacttcct taagcgagtt cgagcggagc gaactcgtcg
    18181 aggtgccctc ggtcggcgga cgcgcgccgc gccaactctc gcgacagatt ctgtgtgaca
    18241 tcctccagcc gcgcgccgag gaggtgctca tgcacgtggc cgacgagata cgtgattccg
    18301 gttgggagcg gcagctctcg tcgggcgtgg tgatgacggg cgggggcgcg ctgctcgacg
    18361 gcataacgga ggtggccgag caggtcttcg acgcgcccgt gcgcgtcggc taccccgagc
    18421 gcgaccgctt cgggggactg atcgaggacg tacagagtcc ggcgtggacg gccgcggcgg
    18481 gcctctgtct gatcgcgcag cgggcgcaga atgcggaaat gcgggccgtc gcggcccggc
    18541 gcgggacgac gggcggactc gtcgccatcg tctcgaaatt ccgcaatcgt tttagtggta
    18601 ttttctgaca atgtgttgta tgctgccgcg ccagccggca caatatgtag tacccaaaat
    18661 attgttgtgg tcggcgcgca cagcccaaga taaccgcacg tgctttccga aggccgtgga
    18721 aagcccagga aagaggaagg actcataact tatgacaccg gaaggccgcc agccgggcag
    18781 tggcattaat tttttcatcg acgaggaccc gcccatcacc ggtgcgcgca tcaaggtgat
    18841 cggcgtcggc ggcggtgggg gcaacgccgt caaccgcatg atcgaggccg gcatcgaggg
    18901 catcgagttc ctcgtcgcca acaccgacct tcaggctttg aagcgctcgc gcgcgcccat
    18961 caagattcaa ctcggcggca aactgaccaa gggactcggc gccggcgcca accctgaaat
    19021 cgggcgcgac gccgcgctcg aagacacgga gaaaatcatc gaggcgctcg aaggcgccga
    19081 catggtcttc gtcaccactg ggctgggagg cggcacgggc accggcgccg cgccgatcat
    19141 cgccagcctg gccacggagc ttaacgcgct gaccgtggcc gtcgtcacca agcccttcca
    19201 cttcgagggc aagcgccgga tgcagcagtc cgagattggg ctgagggagc tgcgcgagtg
    19261 cgtggacacc gtcatcacca tcccgaacga gcgcctgctg cacacggttg accgcaacat
    19321 gtctttgcag gacgccttca agatggccga cgacgtgctg cgtcaggccg tgcagggcat
    19381 cagtgacctg atcaccgtcc ccggactcat caacctcgac ttcgccgacg tgaaggccat
    19441 catgcagggc atgggcctcg ctctgatggg cgcggggcgt gcttcgggcg agcaccgcgc
    19501 catggaggcc acgcagcagg ccatctcgtc gcccctgctc gaagaggcga ccatcgaggg
    19561 cgcgaagggc gtgctcatca acatcacggg cggctacgac ctcacgctct acgaggtcaa
    19621 cgaggccgcg tccatcatcc gcgaggcggc cgacgacgac gccaacatca tcttcggcgc
    19681 ggtcatcgac gagaccctgc gcgacgagat gaagatcacc gtcatcgcca cgggcttcga
    19741 caaggagatc gccgagcttg gcaccaccac ctcggccgcg cacgccgtcg gcggcgccgt
    19801 cgtcccgccg tcgcgctaca cgccgacgcc gacgggcgaa ctcccgccgc gcccctccgg
    19861 gccgccacgc tccgacgacc tcgacgtgcc gaccttcatc cgcaagaagg ctgactgaag
    19921 aaggcaaaag gcaaaagtaa aaaggcaaaa atggatgagc aaaccgctct cccacttttg
    19981 cctttttact tttgcctttt gccttcaagt tgtgtagcat gacgcccatc cctgacagga
    20041 tgaattcaac cgcgggtaaa accgcttctc gtctttctca tcaggagcac agcccgcgcg
    20101 aggggtgacg tgaagtctta cacgagactc cgcccctttg tcggccgggc gcgtgcgcgg
    20161 ccgtgcggcg cgaggccggc ggcaccgccc cgtgccatcc ttttgtactc attcttggag
    20221 gtgaaacagg tgaaccgtaa actcatccgt actacgttgg ctgaggtcaa ggggcgcggc
    20281 gacactcccc agcacaggga gggcggcggt ggcggtggcg gccgcgagtt cgtcagtcgc
    20341 ggcggcgcgc ccagccgggg cgggtcgggc ggcgggccgc cgcgtgatag agattcgggc
    20401 ggctcctccg gcggcgggat gtcggcggcg gccctcggcc gcaagaaggt tccgccggcc
    20461 gagaccaacg cggagatttt ctactacaag aagcagatgg atcagcacac cgagatggtc
    20521 atcgtcctcc tcgacgacga ggagatcgag ggcaccatcg agtggtacga ccgcaacgct
    20581 ctgaagatca accgccaggg cgcgcccaac gtcatgctcc tcaagcacaa catcaagtac
    20641 atgtacaagg ccgaggagcg cgatggcagc gacagcgacg acgcggacga ccgcggcgac
    20701 gacggcgaag acgacgagta gtcgccgccg ccgttcgcgc cgagcgcgtc gcccccgctg
    20761 tcccggccgg taattcctcg ttcacctcac ctgttgtacc atcgcgcaat gaccgacatc
    20821 acgcacacgc agccacacgg cgcgccgccc gccgagggat cggcgcgccg ccggccgcgc
    20881 atcgccgtca cgatgggcga cccggccggc atcggccccg aggtcgtctt gaaagccgtg
    20941 gccgagcgcg cggtgctcga tgcctgcgtg cccgtcatcg tcggcgacgc gcagttcctc
    21001 gcgcacacgg cgcgcaaact cgaccttcag tgcggctacg acatcgtgcg ccgcggcgag
    21061 gcgttccccg ctgacgcgga cggccccgtc atctaccacc tcgacaacat ccgcggcaac
    21121 gtcgagccgg gagtcgagtc ggggtcggct gggcgcgcgg ccgccgagta tatcgaggcc
    21181 gccgtcgagc tgtgcatgac gggcgacgcc gccgccgtct cgaccgcgcc gatcaacaag
    21241 cgctcactct tcctcggcgg ctacagcttc cccggccaca ccgaattcct cgcgcacctg
    21301 acgggggcga ccgactgcgc gatggccttc gtcgcctcga acctgcgtat cgtcctcatc
    21361 tcgacacacg tcccgctggc ggaggccatc cgcatggtcg agcgcgagcg catcgtgcgc
    21421 gtggtgcggc tcaccgagcg cgagctgcgg cggtggggca tcgagcggcc gcggatcgcc
    21481 gtggcggccc tgaacccaca cggcgccgag gggggcctgt tcgggatgga ggaatcggcc
    21541 gagatcgtcc cggccgtgca ggagtgtcag gagcagcacg gcctcgacgc gcgcgggccg
    21601 ttctcggccg acacggtgtt tttgcgcgcc tcgcgcggcg agttcgacgc cgtggtttcg
    21661 tgctaccatg atcaggcgat gatccccgtg aagtgcctct cgttcggcga ggccgtcaac
    21721 gtcacactcg gcctgccctt cgtccgcacc tcggtcgatc acggcacagc cttcgacatc
    21781 gcgggccggg gcgtcgccga gcactccagc atgacggagg ccatcacact ggccgtgcgt
    21841 cttgcgcgcg ccgacgggac gggacaggcc gtggaagtat gaaaaaggtg atgagtgata
    21901 agtgataagt gataagaaag tagcttagct attagctttt agctttcagc acttggctaa
    21961 cagctatgag ctaacagcta acggctctta tcacttatca cttatcacct gtcacttcga
    22021 aaaccggagg ttttcgacag gtgtctgagc aaccaacgca accctcgcgg cgtattctcg
    22081 tggccgacga cgacccggcg atcctgcgtc tggtgacggc gatcatcgaa aaggaaggct
    22141 gcgaggtggt gacggcgcgc gacgggcgcg aggcctacaa gcacctccag tcgggcggcg
    22201 acttcatcgc gggcgtcttc gacgtgatga tgccgcacat tcaggggccg gaactcgtcc
    22261 gttacatgcg cagtgagcgg cgtctgatga agatacccgt gatgatgatg acggccgagc
    22321 agaacccgaa gctctcctcc gacagcttcg ccgccggcgc cgtcgtcttc ctgcccaagc
    22381 ccttcacgac ctcgcagctt caggtcatgc tgcggatgct catcgacaag ggcacccagc
    22441 aaagctcctg agcggcggcg ccgggcgcgc tacggcgtcg gccgccgcga atccccacgc
    22501 gtctccccac gcgacgtgag ccttcccggg cgcgcgcggc cggacttgaa aaccgaggag
    22561 acacgagatg cgacaagctc gaaacaatgc gacgcgcttc gacgccagcg ggagtcggac
    22621 gactcacacg ggtctgctcc tgctggccct cgcgctgctg tgcgtcgtgg ccgccgcgac
    22681 ggcgggcgcg cagtcggggc gacgcaagac ggcgccgatc agccccgtcc ccacgccgac
    22741 gcctgccgcg cccgaggcgg agggcgagtc tgagtcggag agccggtcgc ggcggcaagt
    22801 cggcggcccc gctcaactct ctctcgtcgt ctaccggctc gacaacgctt tcccctacgc
    22861 cgattacaac gtggaagacg tgttgatggg cggtcttatg gatcgcctca cgcgctcgaa
    22921 ggacttgtcg gcgagccgcg gcggcaagcg catctcgcgc aaggaggcgc aggagatcgc
    22981 caaaagcgag agccaatcgc acgtcgtcct cgtggaattg gaggaggaca tcggggcgag
    23041 cggcggtcgc agcgggggag ccgggcagac cgacacgcgc acgctcgccg tcaagaccta
    23101 cgtctacgag atggggacgg ggacgctcaa gttcgtcgat cagacatcgc agcggcccta
    23161 tcgcgggcag acgacgagca tcggcgggat acgcctgccg ctgcccacga cgcggacgcg
    23221 tatggagcgc ttccccggcg agctgcaact cgaacaggcc gcgcgcgacg ccgccgaccg
    23281 gctcatgcac agattcaaac tgcgtccgcc gcccgacaac tgagccgccg cccgccgaag
    23341 cgcccgccgc ctgtcggctt gagtgcggcc ggagtgaaat gcgtctcaag gctcgaagcg
    23401 ccccaatacg cggcgcgcgg aggacaaaag aatttgagat ttgaagtttc aaatttgaac
    23461 tcattcaacc ctccgctccc cttgcgcgtc ctccgcggct ctgcggtgaa ccacccgccg
    23521 acctcctttc aagttaaatt ccacgcggga cgcccgctca cgttgctgct gtgtgcgatg
    23581 cttctcgtca tcccttcggc ctcattcgcc acagagaaga aacccaagaa ggccatcaag
    23641 gatagcgacg cgcggaaggt gatcgccgcc acgcccggct tcgaccttcg cacgggccgg
    23701 gtgcgtgtga aagacgtgtc gctcgcgggc gcgacgcccg tcgtcgtcaa cgccgaggtc
    23761 gagatcgc
//
LOCUS       DASVES010000388        12907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_648336, whole genome shotgun sequence.
ACCESSION   DASVES010000388 DASVES010000000
VERSION     DASVES010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648336"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..778
                     /locus_tag="VF240_15815"
     CDS             164..778
                     /locus_tag="VF240_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176732.1"
                     /translation="MELTERQRDAVSELINIAFARTGAALSELTGHRVLLNAPDVSVL
                     PTSELPAALADFIPGEVASIHQVFGGPVAGDALLLLNQDGALRLTDLLTDGQSPSDRL
                     DESAREVLTEVGNILLNACLGMFGNLLDVRVSFSVPRLHIETLDELLASLTTGEGGPR
                     YALVIYTAFHVRDSSVKGFLVMILSVASLDRLIQEVEAWEGRQD"
     gene            823..2481
                     /locus_tag="VF240_15820"
     CDS             823..2481
                     /locus_tag="VF240_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176733.1"
                     /translation="MTRTSPNEETLLNAALLRWMNDLAGHGILITDAELNIRAWNRWL
                     EEHSELGADEVVGRNLLEVYPELEKRGLYRHYKWILEGQTRILSQRLHGHLLPLPTDP
                     NLSGFSEMQQTARISPLIHDGRVIGTLTVVEDVTERVAREAELQAQVDARTQAFAREK
                     AAREEAEGANRHKDEFLATVSHELRTPLTSILGWSNMLLAGILSGDAHTRALETIHRN
                     AEALNQLISDLLDVSRIISGKLRLDLRTVELHTIIESAVEATRPAAEAKGIRLTAVLD
                     PRAGPINGDADRMQQVIWNLLTNAIKFTPADGQILVKLERAGSRMEMTVRDSGIGIHP
                     EFLPHIFDRFRQADPATNRVHGGMGLGLSIVHQLVELHGGTVRAESEGVGKGAAFVIS
                     LPCFDFEGGSEGAGRLPLAAVGQLEIDCPPSLQGLRVLVVDDEADTLDMIRAVLEHCK
                     MEVITAGSASEALEAIIQLRPDVLLSDLGMPEEDGYALIAKVRALPAEGGGQIPAAAL
                     TAYVRAEDRVKVLRSGFQLHMPKPFEPHELVSAVANLAGRLNDG"
     gene            3033..4238
                     /gene="cax"
                     /locus_tag="VF240_15825"
     CDS             3033..4238
                     /gene="cax"
                     /locus_tag="VF240_15825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX7176734.1"
                     /translation="MPTSSTAKPDSANPVPTPPSRSWLRPSLNWLLVFVPIAIALRFV
                     PALHNPTALFVVSCIAIIPLAGWMGRATEHLAEHLGQGVGGLLNATFGNAAELIIALF
                     ALSKGLTGVVKASITGSIIGNILLVLGLSFLAGGLKFRKQEFNRVAAGVSATALTLGA
                     IALVIPTVFHQAAAMVPVAQGGWTPAKEQGLSLAIAVVLFLTYALTLVFTLVTHRDLF
                     GGDESKAIGREAGEELTRMEAERGAHAPWSVGKAVGVLVGATAFVALISEFLVGAIEE
                     ARESLGLTEVFVGVIVVAIIGNAAEHSSAILMAWRNKMDISIGIAVGSSLQIALFVAP
                     LLIFASYLFGTPMNLEFTVPEVVAVVASIIIVEQISSDGESNWIEGLQLLSVYAVLGI
                     LFYFLPDAH"
     gene            complement(4235..4627)
                     /locus_tag="VF240_15830"
     CDS             complement(4235..4627)
                     /locus_tag="VF240_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176735.1"
                     /translation="MENNEAAQLEITRGEIAAAKSEVRRAGAGAYVKLARALRGGDAR
                     ARLRAVPGSPKLLLASRLLAWCKPFLVLLSPLSLLAAPSWWWALAGVAAAFLLLNAAQ
                     TEVNLELGARLLAADRKRKRGPGCDDGA"
     gene            4829..5161
                     /locus_tag="VF240_15835"
     CDS             4829..5161
                     /locus_tag="VF240_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX7176736.1"
                     /translation="MYAFEGDVEGGLQTYGEALWWTAMLLTSLGSEYWPRTAEGRALC
                     FILSLYGFAVFGYVTAALATFFVGRDAEDEEAELPGAKSLEDLRAEVAALRVDIRSLR
                     ETRGYGEG"
     gene            complement(5200..5670)
                     /locus_tag="VF240_15840"
     CDS             complement(5200..5670)
                     /locus_tag="VF240_15840"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX7176737.1"
                     /translation="MLDWGLTFARDLALAVWLGGLIVIDLVETPARFRVPAIDRNQVV
                     AVGREVFAALNRTEVIVGALLVAASALLAGRAADVSRKSLAALFGVGVMWLVALAQFF
                     WARPRMSAATKDLDLLNRRPEDARFRVFRRWHKTYVALDLVKIALGLAALGMWV"
     gene            complement(5870..6523)
                     /locus_tag="VF240_15845"
     CDS             complement(5870..6523)
                     /locus_tag="VF240_15845"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX7176738.1"
                     /translation="MGRKAAVRMQRLDITNLIEFNDLKFNPVVLVNEPDLRLVLLCLR
                     AGQQVPEHSAAGGITVQAITGRVTFYEGDEACEMSAGTLVRLDAGRAHRVEAHTDAAL
                     LVTMVKTPRAARPDDGPPAAEREIDLCLLPRSERHPLVFDVFDRLAVGESFIVLNDHD
                     PHPLRMQIDQLREGELGWEYVVRGPDTYRVRLTRIAPPVGDAGPVNSGRAESPAAVG"
     gene            complement(6528..7163)
                     /locus_tag="VF240_15850"
     CDS             complement(6528..7163)
                     /locus_tag="VF240_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX7176739.1"
                     /translation="MINNESYQAKEFNLSGLNGISDQTLEMHFKLYEGYVTNTNTLNE
                     KIAELLFDGKVDQEEMPAYSELTRRLGFEYNGMVLHEYYFENMKRGGSGDGDKASAFF
                     QSAEQSFGSYDIWKADFVSVGKMRGVGWAICYQNPANARLSNHWITLHETGNVSGFAP
                     VLVMDVWEHAFLLDYKPAERPRYIEAFFSNIDWDACEARLLKGAAARSGAA"
     gene            complement(7238..7525)
                     /locus_tag="VF240_15855"
     CDS             complement(7238..7525)
                     /locus_tag="VF240_15855"
                     /inference="COORDINATES: protein motif:HMM:NF016109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop repeat-containing protein"
                     /protein_id="HEX7176740.1"
                     /translation="MEALGWAGTVLVVVAYYPQIHHLYVERCAWGISRLTWLIWLAAS
                     TLLLIYCSLRREVMLAVVQAVNIAAIVTTLLLVRRSKRVCPHHLKIAETTT"
     gene            complement(7617..8540)
                     /locus_tag="VF240_15860"
     CDS             complement(7617..8540)
                     /locus_tag="VF240_15860"
                     /inference="COORDINATES: protein motif:HMM:NF022604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HEX7176741.1"
                     /translation="MKSHMPQDAPSGRGISRRKILTAGGATLVGATLAGPLLETVLGQ
                     AMNGQDMRRRGIMSKEGGTKPTFLPEAGSNDPVAHSLAENLFWNEQMMEHAKFFVMLM
                     PGPELAGPRGRAEQFQQTFAGQLEKARAASLDRGNYAAFNRSTVELVKPYSDFKHKMR
                     DEQAAGRLQSLVWPSFFEHTAREAERFAKRLDQFSRGDTSTDMKEAAEFWTIIMGEHA
                     DFIAHLLDPEENALIQKAMQTSAAFRQMHKSVPSSKGPVERAVDDIIDFKTAAERGIQ
                     TGKIRSIIHPALADHVRREALKAADELKRAA"
     gene            complement(8681..10312)
                     /locus_tag="VF240_15865"
     CDS             complement(8681..10312)
                     /locus_tag="VF240_15865"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7176742.1"
                     /translation="MTHKEITIAKTGELKDGEMRQVSAGGTDILLARVGGKYHAVGAH
                     CTHYGAPLVEGALCGERVVCPWHHACFNVATGDLEEPPALDALPRYGVRVERGSVIVS
                     LSDEITDRRTPAMTKRDSSSDARLFVILGGGAAAYAAAQTLREDGFKGRVLMITREDR
                     APYDRPNLSKDYLQGRAEPEWIPLRADEFFAEHDIEVLRDKEVVRVDAAAKTVTFAGG
                     NTLACDALLVATGGTPRRLGIPGSGLKNIYVLRSFADADALVEAAGRASRAVVIGASF
                     IGMETAASLTERGLPVTVVAPGGVPFEKTLGAEIGGLFRRVHEARGVRFKLGAGAARF
                     EGDGAVEVVVLDSGERVEADLVVVGVGVRPATDFLEGVQLHEDGGVYVDRHLRAADGV
                     YAAGDIAYFPSSPTGERQRIEHWRTAQQQGRAAAHNMAGKTVEFEGVPFFWTRQFDAG
                     LIYVGHAASWDEIIFEGDVPSQDFLAFYVKDERVLAVAGMNRDCEMAGIEELMRLDRM
                     PSPARLRDGPVDFLELLTDDGAGDPLSRDAVVVMV"
     gene            complement(10519..11097)
                     /gene="ribA"
                     /locus_tag="VF240_15870"
     CDS             complement(10519..11097)
                     /gene="ribA"
                     /locus_tag="VF240_15870"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX7176743.1"
                     /translation="MAEARLPTDFGEFRIVGYRSLNSDEEFVALARGELRADRPTLVR
                     IHSQCLTGDVFGSVRCDCGRQLRAAMELIGRDGCGAVVYQLQEGRGIGIINKIRAYAL
                     QDEGADTVEANERLGLEVDLRRYDQCAEILLDLGLRRVRVMSNNPAKIQAIGRAGMEA
                     VERVALEVEPHDGFAHYLRTKKERMGHLIHAA"
     gene            complement(11218..12048)
                     /locus_tag="VF240_15875"
     CDS             complement(11218..12048)
                     /locus_tag="VF240_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176744.1"
                     /translation="MSKVLLVSDDPSLREGIREALAGGGYAIQCAPDGRRGAVMLAEE
                     PTDLIITDWTTTEGDAVESLRRLRFEYHNAKRLAVVEGGTPEAVIDALRWHVCDFLTR
                     PFTADELRAAVRGALDGCPAARVEVVSAHPGWVELLAPGDLAAISPLQKLLAQLDADL
                     PPEVHEAISYAFREMLSNAVEYGCRLDPAQRVEVSYVRLGRAIVCRIKDPGAGFDPAR
                     LAHAAINNPDDDPLRHAALREERGLRAGGFGIMLTSRLVDELAFNERHNELMFVKYLS
                     "
     gene            complement(12081..12512)
                     /locus_tag="VF240_15880"
     CDS             complement(12081..12512)
                     /locus_tag="VF240_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176745.1"
                     /translation="MEHIFEGTPAADFSSDAQTRVSSPGYQAYQILRLGFTVAPILAG
                     LDKFFGLLVNWDQYLPPFVNNLTGGHGHELMLAVGVIEIVAGLGVWFKPKIFAYVVSA
                     WLLLIIFNLLMIPGYFDVALRDFGLALGALALARLSHEFDR"
     gene            12665..>12907
                     /locus_tag="VF240_15885"
     CDS             12665..>12907
                     /locus_tag="VF240_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX7176746.1"
                     /translation="MQTTKLDERFFDSTRGRIVSLLRDGSKTVNDLASKLELTDNAVR
                     AHLLTLERDGLVRQSGVQRGARKPHFAYELTAEAERL"
BASE COUNT         2156 a         4305 c         4124 g         2322 t
ORIGIN      
        1 gagatggacg cggacgcgcg cgtgatcgtc gccagcgccg acatccagac ttcgacgcgg
       61 gcgatggtcg aagaggccgg cgcccgcgct ttcatcaaca agccgttcgc cgtggagaac
      121 gtgctcggcg cggtcaactc cgtactgggg gggaagtagc acgatggaac taaccgaacg
      181 ccagcgcgac gcggtctccg agttaatcaa catcgcgttc gcgcgcacgg gggccgcgct
      241 ctccgagttg accgggcacc gcgtcctgct caacgcgccg gacgtctccg tcctcccgac
      301 ttccgagctg cccgcggcgc tcgccgactt catccccggc gaagtcgcct ccatccacca
      361 ggtcttcggc gggccggtcg cgggggacgc gctcctgttg ctcaatcagg acggcgcgct
      421 gcggctgacc gacctgctga cggacgggca gtccccttct gaccgcctcg acgagtcggc
      481 gcgcgaggtg ctgaccgagg tggggaacat tctactcaac gcctgcctgg ggatgttcgg
      541 caacctgctg gacgtgcgcg tctcgttctc ggtgccgcgc ctgcacatcg agacgctcga
      601 cgagctgctc gcctccctca cgactggcga gggcgggccg cggtacgccc tggtgatcta
      661 cacggccttc cacgtccgcg acagctccgt caaagggttc ctcgtgatga tcctgagcgt
      721 cgcctcgctc gaccgcttga ttcaggaagt cgaagcctgg gaagggcggc aggattagtc
      781 ttagacgccg accgtcggcc ggggcgccgg gcatgactcc ccatgacgag aaccagtccg
      841 aatgaagaga cgttgctcaa cgcggcgctg ctgcgttgga tgaatgatct cgcggggcac
      901 ggcatcctga ttacggacgc cgagttgaat atccgcgcgt ggaaccgctg gctggaggag
      961 cacagcgaat tgggcgcgga cgaagtggtg gggcggaacc ttctggaagt ctacccggag
     1021 ctggaaaaga ggggtttgta tcggcactac aagtggattc tggagggtca gacgaggata
     1081 ctttcgcagc gcctgcacgg ccacctgctg ccgctgccca ccgacccgaa tttgagcggc
     1141 ttctcggaga tgcagcagac ggcgcgcatc tcgcctttaa tccacgacgg ccgcgtcatc
     1201 ggcacactga ccgtcgtcga ggacgtcacc gagcgtgtgg cgcgcgaggc ggagttgcaa
     1261 gcgcaggtgg acgcgcgcac ccaggctttc gccagagaga aggccgcgcg cgaagaggcg
     1321 gaaggagcca accggcacaa ggacgagttc ctcgccaccg tttctcacga gctgcgcacg
     1381 ccgctcacct ctatcctcgg ctggtcgaac atgcttctcg ccggcatcct gagcggggac
     1441 gcgcacaccc gcgcgctcga aaccatccac cgcaacgcgg aggcgctgaa ccagctcatt
     1501 agcgacctgc tcgacgtttc acgcatcatc agcggcaaac ttcggctgga cctgcgcacg
     1561 gtcgaactgc atacgattat cgagtcggcc gtcgaggcga cccgccccgc ggcggaagct
     1621 aaaggcatcc gcctgaccgc ggtgctcgac ccgcgcgcag ggcccatcaa cggcgacgcc
     1681 gaccgcatgc aacaggtcat ctggaacctg ctcaccaacg ccatcaagtt cacgcccgcg
     1741 gacgggcaga ttctcgttaa gttggagcgc gccggttcgc ggatggagat gacggtgcgg
     1801 gattccggca tcggcatcca cccggagttt ttgcctcata tcttcgaccg ttttcgtcag
     1861 gccgaccccg ccaccaacag ggttcacggg ggcatgggac tgggactgtc catcgtccac
     1921 caactggtgg agttacacgg cggcacggtg agggccgaga gtgagggcgt ggggaagggg
     1981 gcggctttcg tcatcagcct gccctgcttc gatttcgagg gcgggtcgga gggggcgggg
     2041 cgattgcccc tcgccgctgt cggtcagttg gagatcgatt gcccgccctc tcttcagggc
     2101 ttgcgggtat tggtcgttga cgacgaggcg gacacgctcg acatgattcg cgcggtgctc
     2161 gaacattgca agatggaagt catcacggcc gggtcggcgt ccgaggcgct ggaggcaatc
     2221 atacagttgc ggcccgatgt gttactcagc gacctgggga tgccggaaga ggacggctat
     2281 gctctcatcg ccaaagtgcg ggcgctcccg gccgagggtg gcggacaaat cccggcggcg
     2341 gccctgaccg cgtacgtgag ggcggaagac cgcgtgaagg tgctccgctc gggtttccag
     2401 ttacacatgc cgaagccgtt cgagccgcat gaattagtat cggcggtggc gaacctcgcc
     2461 ggccggctta acgatggtta atctctttca ggggttgtgt tgcttcggcg aggtctaaca
     2521 atcaaccacc ccctccaaca ccccctaacc cgaacctaca cgtgcccgcg gccctctgtc
     2581 atttaccttc cctgggtgcg agcagagaga actcgattgt gcgctcatcc acgcaaggag
     2641 ggcgcagatc ctccttgcgg ctgcggtgag aaacccgggc ccggcaaaat ttataaatgt
     2701 atggcggaga gggtgggatt cgaacccacg gtggcctttc gaccactccg gttttcgaga
     2761 ccgaagggcc gggccgtgaa gggtcggtaa aatcaacttc agggaggcat cgggagagtg
     2821 ccaatccccg cccgtcacgc caaatatcgc gccacccgga cgccaccgat tcgtcgatcc
     2881 ttcgtggacc caacttaaaa ctggctcaca gcaaatacgc gccggatcgg gagttgtgag
     2941 gactgaaaat cacaatactg accggcaccg catcgattgc ccggcccggc gtcagatttc
     3001 acccccgcgg gcgaatgtgg taaatagttt ccatgccaac atcctcgacc gccaaaccgg
     3061 attccgccaa ccccgtgccc acgccgcctt cgcgctcgtg gctcaggccc tcgctcaact
     3121 ggctcctcgt cttcgtgccc atcgccatcg ccctgcggtt cgtgccggcg ctgcataacc
     3181 cgacggcgct gttcgtcgtc tcgtgcatcg ccattatccc gctggccggc tggatgggcc
     3241 gcgccaccga gcacctcgcg gagcacctcg gacagggtgt cggcgggctg ctcaacgcca
     3301 ccttcggcaa cgccgccgaa ctgatcatcg cgctcttcgc cctctcgaag gggctgaccg
     3361 gcgtcgtcaa ggcgtcgatc accggctcga tcatcggcaa catcctgctc gtcctggggc
     3421 tttcgttcct ggcgggcggc ttgaagttcc gcaagcagga gttcaaccgc gtggccgccg
     3481 gcgtctcggc cacggcgctc accctcgggg ccatcgcctt ggtcatcccg acggtcttcc
     3541 accaggcggc ggcgatggtg ccggtcgcgc aaggggggtg gacgcccgcg aaggagcagg
     3601 gcctgtcgct cgccatcgcc gtcgtgctct tcctgaccta cgcgctcacg ctcgtcttca
     3661 ccctcgtcac gcaccgggat ctgttcggcg gcgacgagtc gaaggcgatt gggcgggagg
     3721 ccggcgagga gctgacgcgc atggaggccg agcgcggcgc gcacgcgccg tggtcggtcg
     3781 gcaaggcggt cggcgtgctc gtgggcgcga ccgccttcgt ggcgctcatc tcggagtttc
     3841 tggtcggggc catcgaggag gcgcgcgagt cgctcggcct cacggaggtc ttcgtcggcg
     3901 tcatcgtcgt cgccatcatc ggcaacgccg ccgagcactc gtcggccatc ctcatggcct
     3961 ggcgcaacaa gatggacatc agcatcggca tcgccgtcgg gtcgagcctc cagatcgccc
     4021 tcttcgtcgc accgctcctg atcttcgcct cctacctgtt cggcacgccg atgaatctgg
     4081 agttcacggt gccggaggtc gtggccgtcg tcgcctcgat catcatcgtc gagcagatca
     4141 gcagcgacgg cgagagcaac tggatcgagg ggctgcaact gctctccgtc tacgccgtcc
     4201 tggggatact cttctacttc ctgccggacg cacactaggc cccgtcgtcg cagccgggcc
     4261 cgcgcttccg cttgcgatcc gccgccaata gccgcgcgcc gagttcgagg ttcacctcgg
     4321 tctgcgccgc gttcagcagg aggaacgcgg ctgcgacgcc ggcgagcgcc caccaccacg
     4381 acggggcggc caacagggag agcggcgaca ggagaaccag gaacggcttg caccacgcga
     4441 gcaggcggct ggccagcagc agcttcggcg agccggggac ggcgcgcagg cgagcgcggg
     4501 cgtcgccgcc gcgcagcgcg cgggcgagtt tgacgtaggc gcccgcgccc gcgcgcctga
     4561 cctccgactt ggcggccgcg atctcgccgc gggtgatttc caactgcgcc gcctcgttgt
     4621 tttccatgag agttcgagat gagaagcgcg ctggcctcct cgctgctcat cctggtgata
     4681 atggcacacc tttctgccgg ttggcctcag gcggcggatt gggagcagat tggtggacgg
     4741 gctgaaatta agatcgaggt cgatgcagcc ggccccctca cgacactcct ccccgttcac
     4801 gcgccagccg cccgcggggg tcgctggcat gtacgcgttc gagggtgatg ttgagggcgg
     4861 gctgcaaacc tatggcgagg cgctgtggtg gacggcgatg cttctgacct cgctgggctc
     4921 ggagtactgg ccgcgcaccg cggaggggcg ggcgctgtgc ttcatcctct cgctctacgg
     4981 ctttgcggtc ttcggctacg tcacggccgc actcgccact ttctttgtcg gccgggacgc
     5041 ggaggacgag gaggcggagc tgccgggcgc gaagtcgctg gaagacctgc gggcggaggt
     5101 cgcggcgctg cgcgtcgaca ttcgctcgct gcgagagact agaggctacg gggaggggta
     5161 acaggtgacc tcaaaagcac ccgggtgagt cctcccccac tagacccaca tccctaacgc
     5221 ggcgaggccg agcgcgatct tgaccagatc gagcgcgacg taagttttat gccaccgccg
     5281 gaagacccgg aagcgcgcgt cctcgggccg gcgattcaac aggtcgaggt ctttcgtggc
     5341 cgccgacatg cgcggcctcg cccagaagaa ctgcgcgagc gcgacgagcc acatcacgcc
     5401 gacgccgaac agggccgcga gtgactttcg cgaaacgtcc gcggcgcggc ccgcgagcag
     5461 ggcgctcgcg gcgacgagca gcgccccgac gatgacctcc gtgcggttga gggccgcgaa
     5521 cacctcccgc cccaccgcca ccacctgatt acggtctatg gccgggacgc ggaaccgcgc
     5581 cggggtctcc acaaggtcga tgacgatcaa cccgccgagc cacaccgcga gcgccaaatc
     5641 tcgcgcgaac gtcagccccc agtccaacat cccttatttc gccccctcgc tccaccgaca
     5701 aacgtctgac atcgccgccg tactccccta ccacccgatc aggccgagcc accgggccgc
     5761 gaagaccgcc gcgacaataa ccaccaggat gacggacgcg cgcgggaact tcttcatcgg
     5821 gcattcgttc gtcgccatta ttcattctcc ccaacattac tcgccgacac taaccgacgg
     5881 cggcgggtga ttccgcccgg ccggaattca ccggccccgc gtcccccacc ggcggcgcga
     5941 ttctggtgag ccggacgcga tacgtgtctg gcccgcgcac gacgtattcc caccccaact
     6001 ccccttcgcg cagctgatcg atctgcatcc gcaaagggtg cggatcgtgg tcgtttaaga
     6061 cgatgaaaga ttcgcccacc gccagtcggt cgaagacgtc gaacacgagc gggtgccgtt
     6121 cggaccgcgg cagcaaacac aagtcgattt cacgctccgc cgcgggcggg ccgtcgtcag
     6181 gccgggcggc ccgcggcgtt ttgaccatcg tgacgagcag cgcggcgtcg gtgtgcgcct
     6241 cgacgcggtg cgcccggccg gcgtcgaggc gcacgagcgt cccggccgac atctcgcaag
     6301 cttcgtcgcc ttcgtagaag gtgacgcggc cggtgatcgc ctgcaccgta atgccgcccg
     6361 ccgcggagtg ttcgggaact tgctgtccag cccgcaggca taaaagcaca aggcgcaggt
     6421 caggctcgtt cacaagcact acggggttga acttcaggtc gttgaattca ataaggttgg
     6481 tgatgtcaag tcgctgcatt cttaccgctg cctttcttcc cattcattta cgcggcgccg
     6541 gatcgcgccg ccgcaccctt cagcaggcgc gcctcgcagg cgtcccagtc gatgttggag
     6601 aagaaagcct cgatgtacct gggtcgctcg gccggcttgt agtcgaggag aaaggcgtgc
     6661 tcccacacgt ccatcacgag cacgggcgca aaccccgaga cgttacccgt ctcgtgcagc
     6721 gtgatccagt ggttcgacag acgagcgttg gccgggttct ggtagcagat ggcccagccc
     6781 acgccgcgca tcttgccgac ggagacgaag tcggccttcc agatgtcgta gctgccgaac
     6841 gactgctcgg cgctttgaaa gaaggcggac gccttatccc catcgccact gccgccgcgc
     6901 ttcatgttct cgaagtaata ctcgtggagc accatgccgt tgtactcgaa ccctaatcgg
     6961 cgcgtcagct ccgagtaagc gggcatctcc tcctgatcga ccttgccgtc gaagagcagc
     7021 tctgcgatct tctcgttcaa cgtgttggtg ttcgtgacgt agccctcgta gagcttgaag
     7081 tgcatctcca gcgtctggtc ggagatgccg ttcaggccgc tcaggttaaa ttccttcgct
     7141 tggtacgatt cgttgttaat cattctctta ttcctttcac ctttctctgg agagctctcc
     7201 gaagtctgtc gcaggtgagc gctagcgccc gttactgtta cgttgtcgtc tcggcgatct
     7261 tcaggtggtg tgggcagacc cgtttgctcc tccgcacgag aagcagggtg gtcacgatgg
     7321 ccgcgatgtt gacggcctgc acgaccgcta acatgacctc ccggcgaagt gagcagtaga
     7381 tcaaaagcag cgtgctggcg gcgagccaga tcagccaggt caaacggctg atgccccacg
     7441 cgcacctttc gacgtacagg tgatgtatct gcgggtagta cgcgacgacg acgagcacag
     7501 tccccgccca tcctagtgct tccatattcc gacccttcgc agacggggtg atcccttcga
     7561 gggggaggtg cgagcgtggc ggccgccctc cctccccggc gtagcgtcaa cgaaggtcac
     7621 gcggcgcgct tcagttcgtc ggcggccttc agggcctcgc gccgcacgtg gtcggcgagc
     7681 gccgggtgaa tgatgctcct gatcttcccg gtctggatgc cgcgctcggc ggcggtcttg
     7741 aagtcgatga tgtcgtccac cgccctttcg accggcccct tcgacgaggg cacgctcttg
     7801 tgcatctggc ggaaggcggc gctcgtttgc atcgccttct ggatgagtgc gttctcctcg
     7861 gggtctaaga ggtgcgcgat gaagtcggcg tgctcgccca tgatgatggt ccagaactcc
     7921 gccgcctcct tcatgtcggt cgacgtgtcg ccgcgcgaga actggtcgag ccttttggcg
     7981 aagcgctcgg cctcgcgcgc cgtgtgctcg aagaagctcg gccacacgag gctttgcagg
     8041 cggcccgcgg cctgctcgtc acgcatcttg tgcttgaagt cggagtaagg cttgacgagt
     8101 tcgacggtcg agcggttgaa ggcggcatag ttgccacggt cgaggctcgc ggcgcgcgcc
     8161 ttctcaagct gaccggcgaa ggtctgctgg aactgctctg cccggccgcg cggcccggcc
     8221 aactccggcc ccggcatcag catgacgaag aacttcgcgt gctccatcat ctgctcgttc
     8281 cagaacaggt tctcggcgag cgagtgggcg acggggtcgt tcgagcccgc ctccggcagg
     8341 aaggtcggct tcgtgccgcc ctccttcgac atgatccccc ggcgccgcat gtcctgacca
     8401 ttcatcgcct gcccgagcac ggtctccaga agtggccccg ccagggtggc cccgaccagt
     8461 gtggccccgc cggcggttaa aattttgcgg cgcgagattc cccggcctga gggggcgtcc
     8521 tgcggcatgt ggcttttcat ctttctctac ctcctgtgtt tgtaagtggc aggcgggagg
     8581 ggccgtctgt caggtttatg ctcgggggcg atgatctaat cggcgggtga cgggccgccc
     8641 gcactttgcc ggcagcgagt gtcggcaccc gccccttccg tcaaaccatg acgacgacgg
     8701 cgtcacggct gagcgggtca ccggcgccgt cgtcggtaag tagctcaaga aaatccacgg
     8761 ggccgtcgcg gaggcgggcg ggcgacggca tccgatcaag ccgcatcagt tcttcgatcc
     8821 ccgccatctc gcaatcgcgg ttcatgccgg cgaccgccag cacgcgctcg tctttcacgt
     8881 agaaggccag gaaatcctgt gagggcacgt cgccctcaaa gatgatttcg tcccagcttg
     8941 cggcgtgtcc gacgtatatg agtcccgcat cgaactggcg tgtccagaag aacgggacgc
     9001 cctcaaattc gacagtcttc cccgccatgt tgtgcgccgc ggcgcggccc tgctgctgcg
     9061 ccgtgcgcca gtgctcgatg cgctggcgct cgcccgtggg cgaggaggga aagtaggcga
     9121 tgtctccggc ggcgtacacg ccgtcggcgg cgcgcaggtg tctgtccacg tacacgccgc
     9181 cgtcctcgtg cagttggacg ccttcgagaa agtccgtcgc ggggcgtacc ccgactccga
     9241 cgactaccaa atccgcctcg acgcgttcgc ccgaatccag cacgacgacc tcgaccgcgc
     9301 cgtcgccctc gaagcgggcc gcgcccgcac cgagtttgaa ccgcacgccg cgggcctcgt
     9361 gcacccgccg gaacagcccg ccgatctccg cgccgagcgt cttctcgaag ggcactccgc
     9421 cgggcgcgac cacggtcacg gggagtccgc gctcggtgag actggcggcc gtctccatgc
     9481 cgatgaagct ggccccgatg acgacggcgc gggacgctcg cccggcggcc tcgacgagcg
     9541 cgtccgcgtc cgcgaagctg cgcaggacat agatgttttt cagcccggag ccggggatgc
     9601 cgaggcggcg cggcgtgccg cccgtcgcca cgagcagcgc gtcgcaggcg agtgtgttgc
     9661 cgcctgcgaa ggtgaccgtc ttcgccgcgg cgtcgacgcg cacgacctct ttgtcgcgca
     9721 gcacctctat gtcgtgctcc gcgaaaaact cgtccgcgcg cagcggaatc cactccggtt
     9781 cggcgcggcc ttgcaggtaa tccttactca ggttcgggcg gtcgtagggc gcgcggtctt
     9841 cacgcgtgat catcagcaca cggcccttga atccgtcctc gcgcagggtt tgcgccgctg
     9901 cgtaagcggc cgctccgccg ccgagaatga cgaacagccg ggcgtccgac gaagaatccc
     9961 gcttcgtcat cgcgggcgtc ctgcggtctg tgatctcgtc ggagaggctc acgatgacgc
    10021 tcccgcgctc gaccctcact ccgtagcgcg gcagcgcgtc gagcgcgggc ggctcctcaa
    10081 ggtcgcccgt cgcgacgttg aagcaggcgt ggtgccaggg gcagacgacc cgctcgccgc
    10141 agagcgcgcc ctcgacgagc ggcgcgccgt agtgcgtgca gtgcgcgccg acggcgtggt
    10201 atttaccccc gacgcgcgcc aacaggatgt cggtcccgcc ggccgagact tgcctcatct
    10261 ccccgtcctt gagttcgccg gtcttggcaa tagttatttc tttgtgcgtc atcagacctt
    10321 ccccaccgcc gggcgatcac tctcttccgg gcttgcgcca atcattcagg agttgctcca
    10381 ggccccgtac aggagacgtc taactttgga cttggaaccc aatgccgggt agcgtgttaa
    10441 cacctaacga ccgcgggtgt taactcctgc cctccctcca agccccttgg tggcggcttg
    10501 ttttaacggt tcatagtttt atgccgcgtg gatgaggtgg cccatcctct ctttcttcgt
    10561 cctgaggtag tgtgcgaagc cgtcgtgcgg ctcgacctca agcgccaccc gctcgacggc
    10621 ttccatcccc gcccgcccga ttgcctgaat cttcgccggg ttgttcgaca tcacccgcac
    10681 ccggcggagg ccgaggtcga ggagtatctc cgcgcactgg tcgtaccggc gcaggtccac
    10741 ctccaggccc aatcgctcgt tggcctcgac ggtgtcggcc ccctcgtcct gcaacgcgta
    10801 ggcgcgaatc ttgttgatga tgccgatgcc gcgcccctcc tggagttgat agacgacggc
    10861 cccgcaccca tcgcgcccga tgagttccat cgcggcacgc agttgccggc cgcagtcaca
    10921 cctgacggag ccgaagacgt cgcccgtcag gcactgcgag tgaatgcgga cgagggtcgg
    10981 gcggtctgcg cgcagctcgc cgcgggcgag cgcgacgaac tcctcgtccg agttgagcga
    11041 ccggtagccg acgatgcgga actcgccgaa gtcggtcggc agccgcgctt cggccaccca
    11101 cacgaccgtc ctcggattcg ataacctgtg gcggtcggga gacgaccggc gacttccgac
    11161 ccgggtaacg tttgacgttt cttcctgctc acttccaaac attttctcgg cccccactca
    11221 ggacagatat ttcacgaaca tcaactcgtt gtgccgctcg ttgaaagcca actcgtccac
    11281 gagtcggctg gtcagcatga tgccgaagcc gccggcgcgc aggccgcgct cttcacgcaa
    11341 cgcggcgtgg cggagtgggt cgtcgtcggg gttgttgatg gccgcgtgcg ccagtcgggc
    11401 cgggtcgaag cccgcgcccg ggtctttgat ccggcagacg atggcccgcc caagccgcac
    11461 gtagctgacc tcgacgcgct gcgccgggtc gagccggcaa ccgtattcga cggcgttgct
    11521 gagcatctcg cggaatgcgt agctgatcgc ctcgtgcacc tccggcggga ggtcggcgtc
    11581 gagctgcgcc agtaatttct gaagtgggga gatggccgcg agatcgccgg gcgcgagcag
    11641 ttcgacccac ccagggtgcg ccgagacgac ctcgacccgc gccgccgggc acccgtcgag
    11701 tgcgccacgc accgcggcgc gcagttcgtc ggcggtgaaa ggtctggtca ggaagtcgca
    11761 gacgtgccag cggagcgcgt cgatgaccgc ttccggggtt ccgccctcaa cgacggccag
    11821 tcgcttggcg ttgtggtact caaagcgcag acgcctcaat gactcgacgg cgtcgccctc
    11881 cgtcgtcgtc cagtcagtga tgatcaggtc ggtcggctcc tccgcgagca tcacggcgcc
    11941 gcgccgcccg tccggcgcgc actgtatcgc gtagcccccg cccgcgagcg cctcccggat
    12001 cccttcgcgc agcgacggat catcactgac cagaagtacc ttgctcatga gtccccgaat
    12061 gccctccagt ccccgcgact tcagcgatca aactcgtggc tcaggcgcgc gagcgcgagc
    12121 gcgccgagcg cgaggccgaa atcgcgcagc gccacgtcga agtagcccgg aatcatcagc
    12181 aggttgaaga tgatcagtaa gagccatgcc gaaacgacgt aagcgaaaat cttcggcttg
    12241 aaccagacgc cgagcccggc cacgatctcg atgacgccga cggcgagcat caactcgtgt
    12301 ccgtggccgc ccgtcaggtt gttaacgaac ggtggcaggt actgatccca gttcaccagc
    12361 agcccgaaaa acttatctag cccggctagg atgggagcta cagtgaatcc caggcgcagg
    12421 atttggtagg cctggtaacc gggactggag acgcgtgttt gcgcgtcgga agagaaatct
    12481 gcggccggag tgccttcaaa aatgtgttcc accttcttaa ccctcctcgg cttggagcca
    12541 gtaatctttt ccgcgaggtc ttcttttctc ttaaaataca cctttccact aataagtcaa
    12601 tgactatttt gacttattgt gtttttcagt ttttccgtgt atattcgcgg tttagtgagg
    12661 gctgatgcag acgactaaac ttgacgagcg atttttcgac agcacgcggg ggcgcatcgt
    12721 tagcctgctg cgcgatgggt cgaagacggt caatgatctg gcgtctaagc tcgaactgac
    12781 ggacaacgcc gtccgcgctc acctgctcac gcttgaacgc gacggcctcg tccggcagag
    12841 cggcgtgcag cgcggagcgc gcaagccgca tttcgcctac gagctgacgg ccgaggccga
    12901 gcgcctc
//
LOCUS       DASVES010000389         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1621339, whole genome shotgun sequence.
ACCESSION   DASVES010000389 DASVES010000000
VERSION     DASVES010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621339"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..612)
                     /locus_tag="VF240_15890"
     CDS             complement(328..612)
                     /locus_tag="VF240_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX7176747.1"
                     /translation="MILLFLTCAVLLFIFLVVALWWLKRRTSRSGLNLIGRVASVERE
                     LNPEGHVLIDGELWPARTRTPARVERGSMNVRVVGARGHFLEVEPLERGA"
     gene            complement(714..2477)
                     /locus_tag="VF240_15895"
     CDS             complement(714..2477)
                     /locus_tag="VF240_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176748.1"
                     /translation="MLKRIFVTVAIALSLVTLSAGQAARASQATAERVLVLPFQNTSG
                     FKDYNWVGESFADSLSELLNVPGLSVVSGDERGIVYQQLRLPETVIPSRATAIKIGRE
                     ALATLVVVGTYEVVPAADDKSVASIRGSARIIRVNEGRLTGVLMTDGRWATHEYFFGD
                     ALTNLQTMQGKLAYQVLYERDKALPVAQNEILSRASKIPSRAFESYVKGRMTNDKEKK
                     SNYLRNAMIEYERANPGTVYAQAAFELAELYYAQGDWQKAGEHFSMLQSKEAHYAEAA
                     FYAAMSFWKMNDAERALGALIPLAKEAPLTSIYNNTGAISAQAARAAQKPEERERLLQ
                     QALTFLGRASETTPDDPFVRFNYGYALMLAGKQPEAAEQLRGAIKANPRDGQALFLFA
                     KALERAGQTEAAAANDNEARKYFSDYAKAQVEWQKSQSPAALPVKFQLKPTFNRHDYI
                     AELRARDDRVRQETEGTSAQNLLAKAREMYAGGRDDEALQELRRVVMIEPMNAEAYLL
                     TGRIYQRRGDLEPAINQLKTAIFWDNERKFIDAYVLLGRIFLERGDRAMAMSYARSAL
                     QIDPNHQEAIALHRQVELGSR"
     gene            complement(2597..2672)
                     /locus_tag="VF240_15900"
     tRNA            complement(2597..2672)
                     /locus_tag="VF240_15900"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2636..2638),aa:Glu,seq:ttc)
     gene            complement(2839..3594)
                     /locus_tag="VF240_15905"
     CDS             complement(2839..3594)
                     /locus_tag="VF240_15905"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7176749.1"
                     /translation="MTHTSALFNFRRLLLVALLASALAGCSAFPAAQQTRRAPAPTPD
                     KPANTQATPEPPAPSIERATSEPYTGDLSIFEDPEREKNLQIDRVMDLLEISEGRSVA
                     DIGAGSGWFTVRAARRVGAGGSVYAVEINEEFVKHIEKRAAAEQLSNVRAVLGREDDP
                     ALPPSSVDAVLILKTYHEVAQPVRLLSNLRRSLREGALVGVIDREGKGDDHGISKALV
                     IEEAASAGYALVSEHDFVKPDKMDYFLIFRARR"
     gene            complement(3833..>4256)
                     /locus_tag="VF240_15910"
     CDS             complement(3833..>4256)
                     /locus_tag="VF240_15910"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7176750.1"
                     /translation="SGRRAAAAMVEAGAHAATDVTGFALLGHAFELARASSVTLEIDA
                     ARVPLIPGALTLAAEGLLTSGDKSNREYVGEDVALGDGVGKELASLLFDPQTAGGLLI
                     SIEETRAGALLEALRETYPDATSIGRVVARRARLIEVS"
BASE COUNT          700 a         1289 c         1516 g          751 t
ORIGIN      
        1 gccatgtcag ttgatagtgt ggctttctag gagaaacggg cgggaagcgg gctttgaaat
       61 aggtcgcccc aaaagcggga gaacccggcc gggaggccag gttcctgtaa ggagagatgt
      121 gtgattgggt cgctcggcgg gcggctggcg tagcggcagg ccacgctcag agaagtgatg
      181 gcggcggggt cacggtttaa cttcctccgt gcttgccgac aatgtataga gcacgtcgcg
      241 tgccactgga aatatttttc tggaacgggg cgggcattgt gcgtaagggc cgcgacggcc
      301 gacgccgtac acttcggccg gagtgagtca cgcgccccgc tcaagcggct cgacttccag
      361 gaagtggccg cgcgcgccga cgactcgcac gttcattgag ccgcgctcga cgcgggccgg
      421 cgtccgcgtg cgcgcgggcc acagttcgcc gtcgatgagg acgtggccct ccgggttcaa
      481 ctcgcgctcg actgaggcca cgcggccgat gaggttgagt cccgaacgcg acgttctccg
      541 cttgagccac cagagcgcga cgacgaggaa aataaaaagc aggacggcgc acgtcagaaa
      601 gagaagaatc atcgtttcag gtaagtagtc cgtgaagacg aggggcgcgc gccgaagtgt
      661 tgcttctggc gcgcgcccct caagtgttcg tcgtcaacgg tcgccgcccc agcttaccgg
      721 gagccgagtt cgacctgacg gtggagcgcg atggcctcct gatgattggg gtctatctgg
      781 agggcgctgc gggcgtagct catggccatc gcgcggtcgc cgcgttcgag gaaaatgcgc
      841 ccgaggagga cgtaagcgtc gatgaacttc cgctcgttgt cccagaagat ggcggtcttc
      901 agttggttga ttgccggctc gaggtcgccg cggcgctggt agatgcgccc cgtgagcagg
      961 tacgcctcgg cgttcatcgg ctcgatcatc accacgcgcc gcagttcctg gagggcctcg
     1021 tcatcgcgcc cgccggcgta catctcgcgc gccttcgcca gcaggttctg cgcgctcgtg
     1081 ccctctgtct cctgtcggac gcggtcgtcg cgcgcgcgca gctcggcgat gtagtcgtga
     1141 cgattgaacg tcggcttcag ttgaaacttg acggggagcg cggccggcga ctgcgacttc
     1201 tgccactcga cctgcgcctt cgcgtagtcg gagaaatact tgcgcgcttc gttgtcgttg
     1261 gcggccgccg cctccgtctg cccggcgcgt tcgagcgcct tggcgaagag gaagagcgcc
     1321 tgcccgtcgc gcgggttggc cttgatggcg ccgcgcagtt gctcggcggc ttcgggctgc
     1381 ttgccggcga gcatcagcgc gtagccgtag ttgaagcgga cgaaggggtc gtcgggcgtc
     1441 gtctcagagg cgcggccgag gaaggtgagg gcctgctgga gcagacgctc gcgctcctcg
     1501 ggcttctgtg cggcgcgcgc ggcctgtgcg gagatggcgc cggtgttgtt gtagatgctt
     1561 gtgagcggcg cttctttcgc gagcgggatg agcgccccga gcgcgcgctc agcgtcgttc
     1621 attttccaga agctcatcgc ggcgtagaag gccgcctcgg cgtagtgcgc ctccttgctc
     1681 tggagcatgg agaagtgctc gccggccttc tgccagtcgc cctgagcgta gtacaactcg
     1741 gccagctcga aggccgcctg cgcgtagacg gtgccggggt tggcgcgctc gtactcgatc
     1801 atcgcgttac gcaggtagtt cgacttcttc tccttgtcgt tcgtcatccg tcccttgacg
     1861 taactctcga aggcgcgcga gggaatcttc gaggcgcggc tgaggatttc gttctgcgcg
     1921 acgggcagcg ccttgtcgcg ctcgtagagc acctgatagg cgagcttgcc ctgcatggtc
     1981 tggaggttgg tgagcgcgtc gccgaagaag tactcgtgcg tggcccagcg cccgtcggtc
     2041 atcaagacgc cggtcagtcg cccctcgttg acgcggatga tccgggccga gccgcggatc
     2101 gaggcgaccg acttatcgtc cgcggccggg acgacttcgt aggtgccgac gacgacgagc
     2161 gtggccagcg cctcgcgccc gatcttgatg gccgtggcgc gcgaggggat gaccgtctcg
     2221 ggcagacgca actgctggta gacgatgccg cgctcgtcgc ccgagacgac cgagaggccg
     2281 ggcacgttga gcagttccga gagcgagtcg gcgaagcttt ctcccaccca gttgtaatcc
     2341 ttgaagcccg aagtgttctg gaagggaagc accagcacgc gctcggccgt ggcctgcgag
     2401 gcgcgcgcag cctgccccgc ggagagggtc acgagggaga gggcgatggc gactgtgacg
     2461 aagattcttt taagcatcga tatcaaacct gccagtccgg caaaaagtcg agaccgccaa
     2521 cgcggagtct ggtgtgcgct ggcggcctct cataacccgc gaaaggagca gttctaaaac
     2581 gcggccgtcg aaagtgtggc gtcccgtacg ggagtcgaac ccgtgtcgcc gccgtgaaag
     2641 ggcggtgtcc taggccacta gacgaacggg actcgcttcg actcggcgga cgattcgagc
     2701 taacttaccg tacaggttag gaggtgtcaa ccacacccga cgcgcggctt gggcgcacga
     2761 cttttgatgt ccgggccagc ggaaaagtat gctcaaaacg cggaaaaaga acgggaaggc
     2821 ggcgcgtgcg cgcacatctc agcggcgggc gcggaagatg aggaagtagt ccatcttgtc
     2881 gggcttgacg aagtcgtgct cggagacgag ggcgtagccg gcgcttgcgg cctcttcgat
     2941 gactaatgct ttcgagatgc cgtggtcgtc gcccttgccc tcgcggtcga tgacgccgac
     3001 gagcgccccc tcgcgcagtg agcggcgcag gttcgacagg agtcgcaccg gctgcgcgac
     3061 ctcgtggtag gtcttcagga tgaggacggc atcgacactc gacggcggga gcgcggggtc
     3121 gtcctcacgg cccagcacgg cgcggacgtt cgagagctgc tctgcggcgg cgcgcttctc
     3181 gatgtgtttg acgaactcct cgttgatctc gacggcgtag actgagccgc ccgcgccgac
     3241 acgccgcgcc gcgcggacgg tgaaccagcc cgagccggcg ccgatgtcgg ccacactccg
     3301 gccttcactg atttcgagca agtccatcac gcggtcgatc tgtaaattct tttcgcgctc
     3361 ggggtcttca aagatggaga ggtcgcccgt gtaaggctcg ctcgtcgcgc gctcaatcga
     3421 cggcgccggc ggctcgggcg tcgcctgcgt gttcgcaggc ttgtcgggtg tcggcgcggg
     3481 cgcgcgacgc gtctgttgag ccgcggggaa ggccgagcac ccggcgagcg cggaggcgag
     3541 cagcgcgacc aacaggagac ggcgaaagtt gaagagtgcg gacgtgtgag tcacgtcctt
     3601 atgttagccg aaagtggccg ggatgaaagc cgcagagacg cgcaaatgtc tccggcgaac
     3661 gtaaaaaatt gggtagtggc ccaagttggg gaggcggggc ggaagcccga ccgtcaggga
     3721 gggcgccgcg tgcgtgggcc gcaagcgccc tccctgacgg tcgggcttcc gcctctatct
     3781 ccccgagtga acccactttc aaaaattgta aaggggagtg gacgcggcgg ccttagctaa
     3841 cttcgatgag ccgcgctcga cgcgcgacga cgcggccgat acttgtggcg tcggggtaag
     3901 tttcgcgcag ggcttcgagg agcgcgccgg cgcgtgtctc ttcgattgag atgaggaggc
     3961 cgccggccgt ctgcgggtcg aagaggaggc tcgcgagttc tttgccgacg ccgtcgccga
     4021 gcgccacgtc ttcgccgacg tactcgcggt tcgacttgtc gccgctcgta agcaaccctt
     4081 cggcggcgag cgtgagcgcg ccggggatga gggggacgcg cgcggcatca atctccagcg
     4141 tgacggagct tgcgcgcgcc agctcgaagg cgtggccgag gagggcgaag ccggtcacgt
     4201 ccgtcgccgc gtgcgcgccc gcctcgacca tcgcggccgc cgcccggcga ccggag
//
LOCUS       DASVES010000390        12055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_282564, whole genome shotgun sequence.
ACCESSION   DASVES010000390 DASVES010000000
VERSION     DASVES010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282564"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VF240_15915"
     CDS             complement(<1..938)
                     /locus_tag="VF240_15915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7176751.1"
                     /translation="MNIGQIVGNYKIEQKLGEGGMGAVFRGIDMMLERDVAIKVLRPE
                     LASQPQVVERFRSEAVTVAKLNHPNVATLYSFFRQGEDFLMVMEFVRGVTLDHVIEQR
                     GAIPPEQAVPLFCQVLDGIQHAHDFGIIHRDIKPANMAVTEKGTLKVLDFGIARLLGT
                     ARMTRQGSVIGTIEYMSPEQVKGLETDARSDIYSLGMLLYEMLTGRIPFDLTNEFELM
                     RAQIEQMPIRPRQLNADIPEVVEQAIWRAIAKSPDDRFQSASDFRGELLDAGFAAGAR
                     LAPLTGGASAFTTRPLGQPPASNPSLGYAGAPTQTDA"
     gene            complement(935..1792)
                     /locus_tag="VF240_15920"
     CDS             complement(935..1792)
                     /locus_tag="VF240_15920"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX7176752.1"
                     /translation="MSKRTRSGEAAAERAAYDIVASVRTDKGCVRETNEDSGQMFRPA
                     DAAVLRERGVLVVVADGMGGHSSGEVASRMAVELVSRLYYEADGDAQEALSGAVAEAN
                     RQIYAASQEDEGRQGMGTTCTALALLGGAAFAAHVGDSRLYMLRAGQIYQLTEDDSHV
                     MELVRHGLISKEEARTHEDKNVILRALGTAPEVEVSAVSPVEAREGDYYLLSSDGLHD
                     LVRDEEIARVLAGAADVHAAAEQLIALAKERGGHDNITVGIVGLASPAASLPEAQNMR
                     ATREVEAAR"
     gene            complement(1899..3179)
                     /locus_tag="VF240_15925"
     CDS             complement(1899..3179)
                     /locus_tag="VF240_15925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7176753.1"
                     /translation="MSRSLLINQSIGEYRIESFLGAGGMGEVYRASHSKIGRLAAVKV
                     LTSATQEGGGFVERFFNEARIQASLQHPNIATLYDFCEVAGQPCIIMEYVDGLTVAER
                     IAAHGAPLPPSETARVFEKVVEAIEHIHRHGIIHRDIKSNNIKVSSSGQVKLLDFGIA
                     KGQTSPGLTQVGSVIGTIQYLAPELICGGTADGRVDIWALGVLLYEMVTGRVPFDADS
                     LGELCDQIKRARYVAPAQLNPAVRPEVARVIERCLKENPAERYQSAGDLLRDARRLAT
                     APPPHSRPDETASEESSERETEKTGAAKTIPLIAAAAVVVVLGIVGLVALFVLTSTPA
                     GAPEPAAAKTAVPQGAARSAPADAEERAVELQVSDGRAEVYRDGKLLGTTPYNLKARV
                     GEHVRLTLRRPGYTDEPVAFTVSEKKSYTFSMSK"
     gene            complement(3253..4953)
                     /locus_tag="VF240_15930"
     CDS             complement(3253..4953)
                     /locus_tag="VF240_15930"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7176754.1"
                     /translation="MERIVLRHLSGSKANQVEEFPLNHVKELIFGRDPSATVKYDPDR
                     DDLVGRQHARITQDPNDPSQFIITDLNSRNGTFVNRQRLTGTSRLNIGDAVQFGPGGP
                     EFVFEVEPRPAGAPKATRAVSTGSMAPTMHGPPTREASVPGSGAPAFNPQPNPAGGVK
                     SGVGKATVERMISHNVTEAKKKQGRGFLLAGGAALVVVVGLLAAVGGFFIWRARSAAS
                     TEVAGVKQAIDAERAGEKAVAPRKANEIVKDFGGAVVKISASWRLVSQQGSGLVYHQY
                     MNIQGAPRAVYVRINDTKIEPYLTYNSNPLNVGIGGAHSGSGFAVSSDGFILTNRHVG
                     AAWETAYEFPPSANGGVILGQGPDGKMAVLGVVEQAPRDWVPARSKQDNIAYTGRNDR
                     LDVTFPKSDRRLEAKLSAVSPRHDVAMIKVDSPGAVPKVELNDNYDSIQPGETSIVLG
                     YPAISPPVLGIVRSQDIFNTDTEVREIPDPTVTVGNIGRVLRSQEATGGQDKQLVSIF
                     GDAYQLQINTTGAGNSGGPVFDEQGRVIGIFYAGRSVGTTSITFAVPIRYGRELMGLK
                     "
     gene            complement(5481..6371)
                     /gene="panC"
                     /locus_tag="VF240_15935"
     CDS             complement(5481..6371)
                     /gene="panC"
                     /locus_tag="VF240_15935"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX7176755.1"
                     /translation="MEIINRRQRMASVARKLRREEDKTIGLVPTMGALHEGHLSLVRE
                     ARRMCDVVVVSVFVNPAQFGPGEDFARYPRDLTKDTTQLSDYNVEYIFAPAVEEIYPK
                     NFATYVTVEGLSEQFEGASRPGHFRGVATVLTVLFNTVRPDFAFFGQKDAQQTLVVKR
                     LVRDLAFDTEVVVLPTVREESGLALSSRNNYLSREERDAAVVLYRALLRARESYREGE
                     RSARKIADAVRTAVEAEPRARLDYVGVTDAETMEKLERATDDRAVLVAVAAFVGKTRL
                     IDNIIIQPARERRATGALGG"
     gene            complement(6466..7362)
                     /gene="panB"
                     /locus_tag="VF240_15940"
     CDS             complement(6466..7362)
                     /gene="panB"
                     /locus_tag="VF240_15940"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7176756.1"
                     /translation="MVYLKQQRPEKVTVPSLRASKERGERLVCLTAYDYPTARIVDEA
                     GTDIILVGDSLGNVVLGYDSTVPVTLDEMVAHTRSVRRGVERALLVADMPYGSYHTGA
                     DDAVRAALRLVKDGGAEAVKLEGGRARVEVVRRLVGEEIPVMGHLGLTPQSLNKLGSY
                     RLQAKTANAARQLVEDAQALEEAGVFAIVLEVVPGEVAKVVTETLRVPTIGIGAGPDC
                     DAQILVLHDLVGLSFSRTRPRFVRQYADLRAAFTEAIGRYADDVRTGAFPSEAESYPL
                     PADAAAELNLPAPAGDKKTEKP"
     gene            complement(7567..8088)
                     /gene="folK"
                     /locus_tag="VF240_15945"
     CDS             complement(7567..8088)
                     /gene="folK"
                     /locus_tag="VF240_15945"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7176757.1"
                     /translation="MNEEVEKDLTAYVGLGSNLGDRAGNLLLGVRGMLASGLRVARLS
                     CVYETEPVGVTGQPAFLNMVAELSGPLPTPEQLLARLLRIEHAAGRTREVAHGPRTLD
                     LDLLLYGDHEQQTDLLILPHPRLHLRRFVLAPLAELIPEGTHPALHQTFAQLLAAVDD
                     NAAVGRWQPTGLI"
     gene            complement(8133..8684)
                     /locus_tag="VF240_15950"
     CDS             complement(8133..8684)
                     /locus_tag="VF240_15950"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEX7176758.1"
                     /translation="MDDTLAAHYLENALRNFRELKSLAEKALAQVGDDEFFRQIDDES
                     NSLALVVKHMAGNLRSRWSDFLTTDGEKPDRRRDFEFALAPGDTRASIHEAWEHGWAI
                     LFDSLGSLSPADLLRKVRLRGEPYTVVEAVNRQLTHYGQHTGQIIFLAKHLRSKDWKT
                     LSIARGASETFNAEMKEQFDGER"
     gene            8714..10411
                     /locus_tag="VF240_15955"
     CDS             8714..10411
                     /locus_tag="VF240_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176759.1"
                     /translation="MSQLGALVWLKWRLFRNSMRSRKAVAGRLASSLATLAALVFSLS
                     VAAGLGAAAYFVSSPEALEDPELSRLTGGAFVFFFFALTFIYLMWAVVPLGLEGGSRF
                     EPRRMLLYPISLGKLFAVDFLSELTNLSSVFAVPVVGAICLGAGLARGATARALGLAA
                     LALVFGVVLSKLCSTFVGSLMAGRRTRGEALLAIMGAVLGLSGVLMGQLIESYTRYAA
                     RYEGALRGLRWTPAGALSVGLTDGLAAGSGDAAYFQAAATLAVYTAAAVVLTYMIARR
                     TALGVGGAKKSAARQAAAGRAAAANGGGWELPWMSAELSAVVEKELRYALRNAQLRGL
                     ALMAVSLTVVMRLASGRGSMGRLWPGASEYTEGMQMTYGVLYVFLMVSALTTNLFGYE
                     GAGMRTYVLAPVERRVILLGKNLAMTLIIVVLATVSVAANAIFFRDLSWLALLATALS
                     SVTFASLFALGGNWLSINFPKRIDIGRRMNRSGVAGLLILPLIVVLAAPPAAASLAGW
                     GAGSVVLRYVILALFALASLALYSVVVRRQARRYSEREVEIMEAVTGRDDDSGQVMS"
     gene            10528..11895
                     /locus_tag="VF240_15960"
     CDS             10528..11895
                     /locus_tag="VF240_15960"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEX7176760.1"
                     /translation="MARRKYNRNQRHAPATDRSFQEYVFSVPAQQTSRTELMRLVRGG
                     EDSFLELKVKLSNPERIAQGIVALANTGGGVIVFGVNDNMGVEGVDDAEAVRDELVRL
                     CREEAVPALMPYIDIVSFDSGRRVVAIEVEGKRRPYRTRDGRFFLRVGAEKREATREE
                     LSELLDEARPLGHENLMAVGARVEDIDESLLWSFVREFDGDTAPGRTLSAQGYPVGEV
                     LERDLLLGAGVGDGLGAAGAEVVPTVAGLLLFGRDERVSALLPRSVLTATRYAGDNAQ
                     APKVETVELAGNLHTLFESALRFVERYCDLWDTRPRAFSGRATEDAAAASAEAESDAP
                     VAARANYHRGSVREALANALAHRDLALRDVTTRLNVFDRSVELVNPRRAAGFTPAMQR
                     ALRYGVPQRLNPQLAALFASPAYGLGLAPGGLPALLRDSRLFSGRKTEIHLFNDEFRL
                     RLHGA"
BASE COUNT         1781 a         3828 c         4310 g         2136 t
ORIGIN      
        1 gcgtccgtct gcgtgggcgc gcccgcatag ccgagcgacg ggttgctcgc gggcggctgg
       61 ccgagcggcc tggtcgtgaa ggcggacgcg ccgccggtga gcggggcgag gcgcgcgccc
      121 gcggcgaagc cggcgtccag aagctcgccg cggaagtcgc tcgcgctctg gaagcgatca
      181 tcgggcgact tggcaatggc ccgccagatg gcctgctcga cgacctcggg gatgtcagcg
      241 ttgagttggc gcggccggat gggcatctgc tcgatctgcg ccctcatcag ctcgaactcg
      301 ttcgtcaggt cgaaggggat gcgccccgtg agcatctcgt agagaagcat gccgagagag
      361 tagatgtcgg agcgcgcgtc agtctccaga cccttgacct gttcgggcga catgtactcg
      421 atggtgccga tgacgctgcc ctgtcgggtc atgcgcgccg tcccgaggag gcgggcgatg
      481 ccgaagtcga gcaccttgag cgtgcccttc tcggtgacgg ccatgttggc cggcttgatg
      541 tcgcggtgga tgatgccgaa gtcgtgcgcg tgctgaatgc cgtcgagcac ctgacagaag
      601 agggggacgg cctgctcggg cgggatggcg ccgcgctgct cgatgacgtg atcgagcgtg
      661 acgccgcgga cgaactccat caccatcagg aagtcttcgc cctgacggaa gaagctgtag
      721 agggtggcga cgttcgggtg gttgagcttg gcgaccgtga cggcctcgga gcggaagcgc
      781 tcgacgacct gcggctggct ggccagttcg ggcctgagca ccttgatggc cacgtcgcgc
      841 tcgagcatca tgtcgatgcc gcggaagacg gcgcccatcc cgccctcgcc gagcttttgt
      901 tcgatcttgt agttcccgac gatctgtccg atgttcatct cgcagcctcc acctcgcgcg
      961 tcgcgcgcat gttctgggct tccgggaggg acgcggccgg ggaggcgagg ccgacgatcc
     1021 cgaccgtgat gttgtcgtgg ccgccacgct ccttggcgag cgcgatcaac tgctccgcgg
     1081 ccgcgtgtac gtccgcggcc ccggccaaca cccgcgcaat ctcctcgtcg cggacgaggt
     1141 cgtgcagccc gtccgagctg aggagatagt agtcgccctc gcgcgcctcg acgggactga
     1201 cggccgagac ctcgacctca ggggccgtgc cgagcgcgcg caggatcacg ttcttgtcct
     1261 cgtgcgtgcg cgcctcctcc ttcgagatga ggccgtggcg gaccagctcc atgacgtgcg
     1321 agtcgtcttc ggtcagttgg tagatttggc cggcgcgcag catgtagagg cggctgtcgc
     1381 cgacgtgtgc ggcgaaggcc gcgccgccga gcagcgccag cgccgtgcag gtcgtgccca
     1441 tgccctgccg gccctcgtcc tcctgcgagg cggcgtaaat ttgccggttc gcctccgcga
     1501 cggcgccgct cagagcctcc tgcgcgtcgc catccgcttc atagtagagg cggctgacca
     1561 gctcgacggc catccggctg gcgacttcgc ccgaactgtg tccgcccatg ccgtcggcga
     1621 cgacgacgag cacgccccgc tcccgcagca ccgccgcgtc cgcgggccgg aacatctggc
     1681 cggagtcctc gttcgtctcg cggacgcagc ccttgtcagt gcggacgctg gcgacgatgt
     1741 cataagccgc cctctcagcc gccgcctcgc ccgacctcgt tcgtttggac attccttgca
     1801 cccctttgtt ggtgataagt gatgagtgat aagtgataag aaaaaccaga ttgctctttc
     1861 ttatcactta tcacttatca cttatcactc atcacttatc acttgctcat gctgaacgtg
     1921 taggacttct tctcgctgac ggtgaaggcc accggctcgt cggtgtaacc cggtcgccga
     1981 agtgtcaggc ggacgtgctc gccgactcgc gccttcaggt tgtagggcgt cgtgccgagc
     2041 agtttgccgt cgcggtagac ctccgcgcgc ccgtccgaca cctgaagttc gacggcccgc
     2101 tcctcggcgt ccgcgggggc ggagcgcgcg gcgccctggg ggacggccgt cttcgccgcc
     2161 gccggctcgg gcgcgcccgc gggcgtcgag gtcagcacga agagcgccac cagccccacg
     2221 attcccagca cgacgacgac ggccgccgcc gcgatcaacg gaatggtttt cgcggcgccg
     2281 gtcttctccg tctcgcgctc ggacgattcc tcgctggccg tctcgtcggg ccgggagtgc
     2341 ggcggcggcg ccgtcgcgag tcgccgcgcg tcgcgcaaca aatccccggc gctctggtat
     2401 cgctcggcgg ggttctcctt cagacagcgc tcgatgaccc gcgcgacctc cggcctgacg
     2461 gccgggttga gctgcgcggg cgctacgtag cgcgcgcgct tgatctggtc gcagagttcg
     2521 ccgagggagt cggcgtcaaa aggcacgcgg cccgtgacca tctcgtagag caaaaccccg
     2581 agcgcccaga tgtctacccg cccgtcggcc gtgccgccgc agatgagttc cggcgcgaga
     2641 tactggatgg tgccgatgac gctgccgacc tgcgtgaggc ccggactcgt ctgtcccttc
     2701 gcgatgccga agtcgagcag cttgacctga cccgaggagc tgaccttgat gttgttcgac
     2761 ttgatgtcgc ggtggatgat gccgtggcgg tggatgtgct ctatggcctc gacgaccttc
     2821 tcgaagacgc gggcagtctc ggagggtggc agcggcgcgc cgtgtgcggc gatgcgctcg
     2881 gccacggtca gcccgtccac atactccatg atgatgcagg gctggccggc gacctcgcag
     2941 aagtcgtaga gcgtggcgat gttcgggtgc tggaggctgg cctggatgcg cgcctcgttg
     3001 aagaagcgct cgacgaagcc gccgccctcc tgcgtggccg acgtgagcac cttgacggcg
     3061 gcgaggcgcc cgatctttga gtgcgacgcg cgatagacct cgcccatccc gcccgcgccc
     3121 agaaaggact cgatgcggta ctcgcctatc gactggttga tgagaaggct tctgctcatc
     3181 ggaaaggatc agggatgagg gatgagggat gaaggatgaa tcggaatctg ttcatccctc
     3241 atccctcatc ctttatttaa gtcccatcaa ctcgcgcccg tagcggatgg ggacggcgaa
     3301 ggtgatggat gttgtgccga cgctgcggcc ggcgtagaag atgccgatga cgcggccctg
     3361 ctcgtcgaag acggggccgc ccgagttgcc ggcgccggtg gtgttgatct ggagctggta
     3421 ggcatcgccg aaaatgctga cgagctgttt gtcctgaccg cccgtggcct cctggctgcg
     3481 gagcacgcgg ccgatgttgc cgaccgtgac ggtcgggtcg ggtatctcgc gcacctcggt
     3541 gtcggtgtta aagatgtcct gcgacctgac gatgccgagc acgggcggcg agatggccgg
     3601 gtagccgaga acgatggatg tctcgcccgg ctggatggag tcgtagttgt cgttaagctc
     3661 gaccttcggc accgcgccgg gactgtccac cttgatcatc gccacgtcat gacgcggcga
     3721 gaccgccgac agcttcgcct ccagccgccg gtcggacttg gggaaggtca cgtcgaggcg
     3781 gtcgttgcgg cccgtgtagg cgatgttgtc ctgcttgctg cgcgcgggaa cccagtcgcg
     3841 cggggcctgc tcgacgacgc cgaggacggc catcttgccg tcagggcctt ggccgaggat
     3901 gacgccgccg ttggcgctcg gcgggaactc gtaggccgtc tcccacgccg ccccgacgtg
     3961 gcggttcgtg aggatgaagc cgtcgcttga gacggcgaag cccgagcctg agtgcgcgcc
     4021 gccgatgccg acgttcaagg ggttgctgtt gtaggtcagg tacggctcga tcttagtgtc
     4081 gttgatccgg acgtagacgg cgcggggcgc gccctggatg ttcatgtact gatggtagac
     4141 aagcccgctg ccctgctgcg agacgagccg ccaggaggcg ctgatcttga cgaccgcgcc
     4201 gccgaaatcc ttgacgatct cgttggcctt tctcggcgcg acggccttct ccccggcccg
     4261 ctcggcgtcg atggcctgct tcacccccgc gacctcggtc gaggcggccg agcgcgcgcg
     4321 ccagatgaag aagccgccga cggcggccag caggccgacg accacgacga gcgccgcgcc
     4381 gccggccagc aggaatccgc ggccctgctt cttcttcgcc tcggtgacgt tgtgcgaaat
     4441 catgcgttct acggtcgcct tgccgacacc gcttttcacc ccgccggccg ggttgggctg
     4501 cgggttgaag gccggcgcgc ccgaaccggg cacgctggct tcgcgcgtcg gcggcccgtg
     4561 catggtcggg gccatcgagc cggtggagac ggcgcgcgtc gccttgggcg cgcccgccgg
     4621 gcggggctcg acctcgaaga cgaactcggg gccgccgggg ccgaactgca ccgcgtcgcc
     4681 gatgttgaga cgcgaggtgc cggtgagtcg ctgccggttg acgaacgtgc cgttgcggct
     4741 gttgaggtcg gtgatgatga actgcgaggg gtcgttcggg tcttgcgtga tccgcgcgtg
     4801 ctgacgcccg acgaggtcgt cgcggtcggg atcgtatttc actgtcgccg aggggtcgcg
     4861 gccgaagatc agctctttga cgtggttgag gggaaactct tccacctgat tggccttcga
     4921 gccgctcagg tgcctgagga cgatgcgttc catggggatg tctcctctaa tctaactttg
     4981 cgagtctggc gccgcgccgc gtgcggttga ttcgtcccgc ccggccaagt gcggaggatg
     5041 gttgagtgtg cgcggcggcg gcgatggcat ttggtttggg aacgagcagg ggcatgctcg
     5101 tggttcacgg cgcgccgtgc cccttcgtgc ccgaagtgtt cgcggccacc gaccgtctac
     5161 cgtctggtct gatttcaatg attcgagcgc gggcaggtaa gattctggaa aaagctgccg
     5221 ggagtaatgt tcatccgcag agagcgaggg ctgagatgaa cgccaccaga gtcgcacgct
     5281 ccaacaatct tccgtcggcc cagcgggcga aattatacat caaactcaat tgaccgcaag
     5341 gattccaccc cgcgcttaaa acgccccggt tcaagtggcc acgcgctccc gagatgtggc
     5401 cgccgtaggc agcccccggc ggcctctcga ccgacgccgc ggcggctgac cgtcactcgc
     5461 ctgagcgccc gcgcgggact tcaaccgccc agtgcgccgg tggcgcggcg ctcgcgggcg
     5521 ggctggatga tgatgttgtc gatgaggcga gtcttgccga cgaaggcggc gacggcgacg
     5581 aggacggcgc ggtcgtccgt ggcgcgctca agcttctcca tcgtctcggc gtcggtgacg
     5641 ccgacgtagt cgagacgggc gcgcggctcc gcttcgacgg ccgtgcgcac cgcgtcggca
     5701 atcttgcggg cgctgcgctc gccctcgcgg tagctctccc tggcgcgcag gagggcgcgg
     5761 tagaggacga cggccgcgtc gcgctcctcg cggctgagat agttgttgcg cgaagataag
     5821 gcgaggcccg actcctcgcg cacggtgggc aggacgacga cctcggtgtc gaaggcgagg
     5881 tcgcgcacga gtcgcttgac gacgagggtc tgctgcgcgt ccttctggcc gaagaaggcg
     5941 aagtcggggc ggacggtgtt gaagaggacg gtcaagaccg tcgccacgcc gcggaagtgg
     6001 ccgggtcgag aagcgccctc gaactgctcg gagagacctt cgaccgtgac gtaggtggcg
     6061 aagttcttcg gataaatctc ctcgaccgcc ggggcgaaga tgtactcgac gttgtagtcg
     6121 gagagctgcg tggtgtcttt cgtgaggtcg cgagggtagc gcgcgaagtc ttcgccgggg
     6181 ccgaactgcg cggggttgac gaagacggag acgacgacca cgtcgcacat ccgccgcgcc
     6241 tcgcgcacga gtgagaggtg gccctcgtgg agcgcgccca tcgtcggcac cagcccgatg
     6301 gtcttgtctt cctcgcgccg cagcttgcgc gcgacggacg ccatgcgttg acggcggttg
     6361 atgatttcca taaaaagtca ggagtcagga gtcaggagtc aggagtcaga ataagaagcg
     6421 agtaaacttt ttcattctga cttctgactt ctgacttctg actcctcatg gcttttccgt
     6481 cttcttgtcg ccggcgggcg cggggaggtt cagctcggcg gcggcgtcgg ccggcagcgg
     6541 gtacgattcg gcctcggagg ggaaggcccc cgtgcgcacg tcgtcggcgt agcggccgat
     6601 ggcctcggtg aacgcggcgc gcaggtcggc gtactggcgg acgaagcgcg ggcgcgtgcg
     6661 gctaaacgag aggccgacga ggtcgtggag gacgagtatc tgcgcgtcgc agtctggccc
     6721 cgccccgatg ccgatggtcg gcacgcgcaa agtctccgtg accactttgg cgacctcgcc
     6781 ggggacgact tcgagcacga tggcgaagac gccggcctct tcgagcgcct gcgcgtcctc
     6841 gacgagctgg cgggcggcgt tggccgtctt cgcctgaagc cggtagctgc cgagcttgtt
     6901 caaggactgc ggcgtcagcc ccagatggcc catcacggga atctcctcgc cgacgaggcg
     6961 gcgcaccacc tcgacgcgcg cccggcctcc ttcgagcttg acggcctcgg ccccgccgtc
     7021 cttgaccaga cgcagggcgg cgcgcaccgc gtcgtccgcc cccgtgtggt aggagccgta
     7081 aggcatgtcg gcgacgagca gggcgcgctc gacgccgcgg cggacggagc gcgtgtgcgc
     7141 gaccatctcg tcgagcgtga cgggcacggt cgagtcgtag ccgaggacga cgttgcccag
     7201 agagtcgccg acgaggatga tgtcggttcc ggcctcatcg acgatacgcg cggtcgggta
     7261 gtcgtaggcc gtcaggcaga cgaggcgctc gccccgctcc ttgctcgcgc gcagggaggg
     7321 cacggtcacc ttctcgggcc gctgctgttt gaggtagacc atcggagaga cccttaaact
     7381 gtttcctgga attcgagggc gccgaattat aggcccgcac ggcgggcggc agcaagaaga
     7441 aagggatgag ggcggaaggt ggaaaggagt acgcacagta gcgcgagcgg tagcgagtgg
     7501 gtctgttcac gcgagacaag ccattagcgc agccgcaccc gctcgctacc gctcgcggta
     7561 ctgtcgttaa atcaatcccg tcggttgcca gcgcccgacc gcggcgttgt cgtccacggc
     7621 ggcgaggagt tgggcgaaag tctggtggag ggcgggatgt gttccttcgg ggatgagttc
     7681 ggcgaggggg gcgaggacga agcggcggag gtggaggcgc gggtgtggga ggatgaggag
     7741 gtcggtctgc tgttcgtggt cgccgtagag caggagatcg aggtcgagcg tgcgcgggcc
     7801 gtgcgccact tcgcgcgtgc gcccggcggc gtgctcgatg cgaagcaggc gcgcgagcag
     7861 ttgctcaggc gtcgggagag ggccggagag ttcggcgacc atgttgagga aagcgggctg
     7921 ccccgtcacg cccaccggct cggtctcgta gacgcaggag aggcgcgcga ctcgcaggcc
     7981 ggaggccaac atcccgcgca cgccgagcag gaggttgccg gcgcggtctc cgaggttcga
     8041 gccgaggccg acgtaggcgg tcaagtcctt ctcaacttct tcgttcataa actgaactgc
     8101 ggcgtcttca ctcatactcc gcgacgcggc attcaacgct cgccgtcgaa ctgctccttc
     8161 atctccgcgt tgaaagtttc ggaggcgccg cgcgcgatgc tcagcgtctt ccagtctttc
     8221 gagcggaggt gcttggcgag gaagatgatc tggccggtgt gctggccgta atgcgtgagc
     8281 tggcggttga cggcctcgac gaccgtgtaa ggctcaccgc ggaggcgaac cttgcggagc
     8341 aggtcggccg gcgagagcga gccgagggag tcgaagagga tggcccagcc atgctcccat
     8401 gcttcgtgga tggaggcgcg cgtgtcgccg ggggcgaggg cgaactcgaa gtcgcggcgg
     8461 cggtcgggct tttcgccgtc ggtcgtcagg aagtctgacc agcgggagcg caggttgccg
     8521 gccatgtgct tgacgacgag ggcgaggctg ttcgactcgt cgtctatctg ccgaaagaat
     8581 tcgtcgtcgc ccacctgcgc cagcgccttc tcggcgagac tcttaagctc gcggaaattg
     8641 cggagggcgt tttcgagata gtgtgcggcg agtgtgtcgt ccatgccggg cattttagta
     8701 taagctgcaa accatgtctc aactgggcgc gctcgtttgg ctgaagtggc gtctgttccg
     8761 caactcgatg cgctcgcgca aggccgtcgc ggggcggctg gcgtcgtcgc tggcgacgct
     8821 ggcggcgctc gtcttctcgc tctcggtcgc cgcggggctg ggagcggccg cctacttcgt
     8881 ctcgtcgccg gaggcgctcg aggacccgga gttgtcgaga ctcacgggcg gcgccttcgt
     8941 cttcttcttc ttcgcgctga cgttcatcta tctgatgtgg gccgtcgtgc cgctggggct
     9001 ggagggcggg agccggttcg agccgcggcg gatgctgctc taccccatct cgctcgggaa
     9061 actcttcgcg gtcgatttcc tgagcgagct gacgaacctc tcgtcggtct tcgccgtccc
     9121 cgtggtgggg gcgatctgtc tgggggcggg gctggcgcgg ggcgcgacgg cgcgcgcgtt
     9181 ggggctggcg gcgctggcgc tcgtcttcgg cgtcgtgctc tcgaagctgt gctcgacgtt
     9241 cgtcggctcg ctgatggcgg ggaggcggac gcgtggcgag gcgttgctgg caatcatggg
     9301 cgcggtgctc ggtctgtcgg gcgtgttgat ggggcagttg atcgagagct acacgcgcta
     9361 cgccgcgcgc tacgagggcg ctctgcgggg tttgcgttgg acgccggcgg gggcgctctc
     9421 cgtggggctg accgacgggt tggcggcagg cagtggagac gcggcttact tccaggcggc
     9481 ggcgacgctg gcggtctaca cggcggcggc ggtcgtcctc acctacatga tcgcgcggcg
     9541 cacggccctc ggcgtgggcg gcgcgaagaa gagcgcggcc cgtcaggccg cggcgggaag
     9601 ggcggcggcc gctaacgggg gcggctggga gttgccgtgg atgtcggcgg agctgtcggc
     9661 cgtcgtggag aaggagttgc gctacgctct gcgtaacgcg cagttgcgcg gccttgccct
     9721 gatggccgtg tcgctgacgg tcgtgatgag gctggcgtcg ggccggggga gcatggggcg
     9781 tttgtggccg ggcgcgagcg agtacaccga agggatgcag atgacctacg gcgtgctcta
     9841 tgtcttcctg atggtctcgg cgctgaccac gaatctcttc ggctacgagg gcgcggggat
     9901 gagaacttac gtgctcgcgc ccgtggagcg gcgggtgatt ctgctgggga agaatctggc
     9961 gatgacgctc atcatcgtcg tgctcgcaac cgtctcggtc gcggccaacg ccatcttctt
    10021 ccgcgacctc tcgtggctgg cgcttttggc gacggcgctc tcctccgtca ccttcgcctc
    10081 cctcttcgcg ctgggcggca actggctctc gatcaacttc ccgaagcgca tcgacatcgg
    10141 tcgccggatg aaccgctcgg gcgtggcggg gctgctgatt ctgccgctca tcgtggtgct
    10201 cgcggcgccg cccgcggcgg cctcgctcgc cggctggggc gcgggaagcg tcgtcttgag
    10261 gtatgttata ctcgcgctgt tcgcgctcgc gtcgcttgcc ctctacagtg tcgtcgtcag
    10321 gcggcaggcg cgccgctact cggagcgcga ggttgaaatc atggaggccg tgacgggtcg
    10381 cgacgacgac tccggccagg tcatgagctg acgcggaggg cgcgcgcgca accgtgcgcc
    10441 cggcccgacg cgtttacaaa tccagaggcg ctgaccgact caagcgcccg ggcgggttcc
    10501 ctacagcgaa gccccgtcca gacacaaatg gctcgcagaa aatacaatcg taaccagcgg
    10561 cacgcccccg ccaccgaccg ctcttttcag gagtacgttt tctccgtccc cgcgcagcag
    10621 acctcgcgca cggagctgat gcggctcgtg cgcggcggcg aagactcctt cctcgaactc
    10681 aaagtaaaac tctcgaaccc cgagcgcatc gcgcagggca tcgtcgcgct cgccaacacg
    10741 ggcggcggcg tgatcgtctt cggcgtcaac gacaacatgg gggtcgaagg cgtggacgac
    10801 gccgaggccg tgcgcgacga actcgtccgc ctctgccgcg aggaggccgt ccccgcgctc
    10861 atgccctaca tcgacatcgt ctcgttcgac agcggccggc gcgtcgtcgc catcgaggtg
    10921 gagggcaagc gccgccccta ccgcacgcgc gacgggcgct tcttcctgcg tgtcggggcg
    10981 gagaagcgcg aggccacacg cgaggagctg tccgagcttc tggacgaagc gcgtccgctc
    11041 gggcacgaga acctgatggc cgtcggcgcg cgcgtcgaag acatcgacga gtcgctgctg
    11101 tggagtttcg tgcgcgagtt cgacggcgac actgcgccgg gccgcacgct ctccgcgcag
    11161 ggctatcccg tgggtgaggt tttagagcgc gacctgcttt tgggggcggg cgtcggcgac
    11221 gggttggggg cggccggggc cgaggtcgtg ccgacggtgg cgggcctgct gctcttcgga
    11281 cgcgacgagc gcgtgtcggc gctgctcccg cgctcggtgt tgacggccac gcgctacgcg
    11341 ggcgacaacg cgcaggcgcc gaaggtcgag acggttgaac tcgccggcaa cctccacacg
    11401 ctcttcgagt cggcgctgcg cttcgtcgaa cgctactgcg acctctggga cacgcgcccg
    11461 cgcgcgttct ccggccgggc cacggaggac gcggcggcgg cgtcggccga agcggagtcg
    11521 gacgcgcccg tcgccgcgcg cgccaactat catcgcggct cggtgcgcga ggcgctcgcc
    11581 aacgcgctcg cgcaccgcga cctcgcgctg cgcgacgtga cgacgcgtct caacgtcttc
    11641 gaccgttccg tcgagctggt caacccgcgc cgcgccgccg gcttcacgcc cgccatgcag
    11701 agggccttgc gctacggcgt gccgcaacgt ctgaacccgc agctcgcggc cctcttcgcc
    11761 agccccgcct acggcctcgg gcttgcgccc ggcggactgc ccgcgctgct gcgcgactcc
    11821 cgcctcttct cgggccgcaa gaccgagata cacctcttca acgacgagtt tcgcctgcgc
    11881 ctccacggcg cctgagaaga cagtgagcag taagcagtaa gctgtaagca gtcaagacaa
    11941 gtaaggccgg ccggagcatc atgcaccggg cggccttctt ggttatccgg cggcatttta
    12001 gtcttctgaa aatggataaa gtcgagttgc gcggtcgaaa taatttgcgt tgaca
//
LOCUS       DASVES010000391         5894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_440311, whole genome shotgun sequence.
ACCESSION   DASVES010000391 DASVES010000000
VERSION     DASVES010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440311"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1240
                     /gene="speY"
                     /locus_tag="VF240_15965"
     CDS             101..1240
                     /gene="speY"
                     /locus_tag="VF240_15965"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX7176761.1"
                     /translation="MAKRKVSKFLSGRRIDPRPITGDEALADLVDETFLAYNAGRLRE
                     ACQLFARKMLEPDVTVGLSLTGALTPAGLGISALIPLMRAGFVDWIITTGANLYHDTH
                     FGIGLALHQGNAQTSDITLREEEVVRIYDIFFDYTVLLDTDAFFRRLIEGEEFQRPMS
                     TAEFHNLCGKYVREREHKLGLENKSFLAAAYEFGVPLYTSSPGDSSIGMNVAAKALQG
                     NKLAFDPSLDVNETAAIVLAAKRGAIHGRGDRGTKHGGKSAVFILGGGSPKNFMLQTE
                     PQIQEVLGIDERGHDYFLQVTDARPDTGGLSGATPGEAVSWGKVDPDRLPDAVVCYVD
                     STIALPVITAYALARHEPRELKRLHDRRGELMDLLRAEYEKSKRR"
     gene            1385..1864
                     /locus_tag="VF240_15970"
     CDS             1385..1864
                     /locus_tag="VF240_15970"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7176762.1"
                     /translation="MAKKITDDQPRVYFDCAKCPAYCCSIYERVSVKKRDITRLAKHF
                     GVSYATAQRRYTTKWQDERVLRRVKDVIFPETCMFLDQEKRCCTIYHGRPGVCREYPD
                     RVRCAYYDVLQFERRQQNDETVIPLVQITFMEVIKTETTNDNEGGTERVWEWEPEEK"
     gene            1965..2189
                     /locus_tag="VF240_15975"
     CDS             1965..2189
                     /locus_tag="VF240_15975"
                     /inference="COORDINATES: protein motif:HMM:NF041655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MM0924 family protein"
                     /protein_id="HEX7176763.1"
                     /translation="MREFLAKMTGKELDVLCSGSPGLRGKVLKIEGGVLHLKDDNKQL
                     CYVAVDHIIAVWEVRADKDQRPGFITHLPK"
     gene            complement(2298..2951)
                     /locus_tag="VF240_15980"
     CDS             complement(2298..2951)
                     /locus_tag="VF240_15980"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX7176764.1"
                     /translation="MSLFKSFPRMCAACALLGLLATTAFAQEARPRIAATTTSTETSG
                     ATRLENEPMVISLATTDEAEPPAATTVAPGLRLSGLNMAILDAIEGRLGTPYRLGSEG
                     PNRYDCSGFVWSVFQEVGVNFERGSARTFWSRFAVPTEEEKYKFGTLVFFNHLGHIGI
                     VADAGGFYHASSSRGVVYSTFNDYWAKRIVGFRRVPPALLETAAANFARRADVAAGR"
     gene            3329..4813
                     /locus_tag="VF240_15985"
     CDS             3329..4813
                     /locus_tag="VF240_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176765.1"
                     /translation="MLSVKSRVTGRRVDAAARTRVLERLQETGFTPLIRTMRLGAFEE
                     ALAAGGEPPKDMERLMAAWTDAESDAPYVRGDLFCFESHIFFLIFADDAASDDAGLRA
                     GIIHDAEADALERQLESFCRNIDESVDAESARGGEGVGGVESGANGGVAQGDAEWRAQ
                     EVSAPESFTRFVASMSEGGAAGLAPPQSARGETAERLRAVEILEDAEARRLLRRLSDA
                     QAEGRTAEMLLGAGREGESLPEGLVRRLAGAALVRRELLISCRKDGRSLFRLPSADAL
                     AVMTASHAMCSECGAAVADERAEELVTPTPLAGSMLKDGAWLVSRMRAALAELGVAET
                     DVATRPATNDSDALMMANAAGESFLFVLRDGDFTLAHARRAIDVEADTEVSHLVVVAT
                     GKVQEDARSRLREHQKRRARTGAELDLLAVEGVDAAAGELRQALERVTQKSLAGELYE
                     LDAGFGMNAGAFVAACFRLTQKPVALQDRAASAAGALAGSLREI"
     gene            5016..5753
                     /locus_tag="VF240_15990"
     CDS             5016..5753
                     /locus_tag="VF240_15990"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7176766.1"
                     /translation="MSSERNGQVFNAGFALAVGLVLASIVFAWAYTRRQGADQTITVT
                     GSAKRRIKSDLVVWRANISYQSAQLAEAYGALKDRVPRVKAYLVSKGVPEDRIVVSSI
                     STKTMQDKDEGGEETGQINGYVLSQTLEVRSDDMEKITKISREVTELIDQGILLESQA
                     PEYHYTKLSDLKIEMLGEAAKDAKVRAQQIASSTGSEVGSVRSARMGVMQITAADSNE
                     VSDYGVNDTSSLEKDIAAVVNVSFAVD"
BASE COUNT         1129 a         1796 c         1983 g          986 t
ORIGIN      
        1 gaatcaggtc tttaagaaaa tcccggcttg ggaagacgtg cagatttcat atgccgagcg
       61 ggagtgagtg atcacgaaag ttgagagcga agggggaggg atggcgaaga ggaaggtgag
      121 caaattcctg tcggggcggc gcatcgaccc gcgcccgatc acgggcgacg aggcgctggc
      181 tgatctcgtc gatgagacgt ttctagctta caacgccggc cgtctcaggg aggcgtgcca
      241 actcttcgcg cgcaagatgc tggagccgga cgtgaccgtc ggtctctcgc tcacgggcgc
      301 gctcacgccg gcgggcctcg gcatctccgc gctcatcccg ctcatgcgcg ccggcttcgt
      361 cgattggatc atcaccaccg gcgcgaacct ttaccacgac acccacttcg gcatcggcct
      421 cgcgctccac cagggcaacg cgcagacctc cgacatcacg ctccgcgaag aggaggtcgt
      481 ccgcatctac gacatcttct tcgactacac ggtgctcctc gacaccgacg ccttcttccg
      541 ccggctcatc gagggcgagg agtttcagcg gccgatgtct acggccgagt ttcacaacct
      601 gtgcggcaag tacgtgcgcg agcgcgagca caagctgggg ctggagaata agtcgttcct
      661 cgccgccgcc tacgagttcg gcgtcccgct ctacacgtca agccccggtg attcgtccat
      721 cggcatgaac gtcgccgcga aggcgctgca aggaaacaaa ctcgccttcg acccgtcgct
      781 cgacgtgaac gagacggcgg ccatcgtgct cgccgccaag cgcggcgcca tccacggccg
      841 aggcgaccgc ggcacaaaac atggcggcaa gtcggccgtc ttcatcctcg gcggcggctc
      901 tccgaagaac ttcatgctcc agaccgagcc gcagattcag gaagtcttag gcatcgacga
      961 gcgcggccac gactacttcc tacaggtgac ggacgcccga cccgacacgg gcggactctc
     1021 aggggcgacg cccggcgagg ccgtctcgtg gggcaaggtt gaccccgacc gcctgcccga
     1081 cgccgtcgtc tgctacgtcg attcgaccat cgcgctgccc gtcatcaccg cctacgcgct
     1141 cgcgcgccac gagccgcgcg agctgaagcg gctccacgac cggcgcggcg agttgatgga
     1201 cttgctccgg gccgaatacg aaaagtcgaa gagaagataa agcgcgtaaa tcgtcaatcg
     1261 taaatcgtga atcgtgaacc ggcggggttg acctttgtcc ggcttgcgat ggataactca
     1321 aggcgattaa ctttcaacca cgattgacga tttacgattc acgatttacg gccttggaga
     1381 gttaatggca aaaaagatta cggacgatca gccgcgcgtc tacttcgact gcgcgaagtg
     1441 cccggcctat tgctgctcaa tctacgagcg cgtctcagtt aagaagcgcg acatcacgcg
     1501 actggccaaa cacttcggcg tctcctacgc gacggcgcag cgtcgctaca ccaccaagtg
     1561 gcaggacgag cgcgtgctgc gccgcgtcaa ggacgtgatc ttccccgaga cgtgcatgtt
     1621 cctcgaccag gagaagcgct gctgcacgat ctaccacggt cggcccggcg tctgccgcga
     1681 gtaccccgac cgcgtgcgtt gcgcctatta tgacgtgctg caattcgagc gccggcagca
     1741 gaacgacgag accgtcatcc ccctcgtcca gatcaccttt atggaggtga tcaagaccga
     1801 gacgacgaac gacaacgagg gcggaacaga gagggtctgg gagtgggaac cagaagaaaa
     1861 gtgataagtg atgagtgata agtgataaga aggaagagag gcgacttctt ctctttctta
     1921 tcacttatca ctcatcactt atcactattt tgggaggtgt tgagatgaga gaatttctgg
     1981 cgaagatgac cggaaaagag ttggacgtgt tatgcagcgg gtcgccgggc ctgcggggca
     2041 aggtcttgaa gatcgagggc ggcgtgctcc acctcaagga cgacaataag caattgtgct
     2101 acgtggccgt tgaccacatc attgccgtct gggaggtgcg cgccgacaag gatcagcggc
     2161 ccggcttcat cacgcacctg ccgaagtagg cggcccaacg gcggtggctc cgcgtgccgc
     2221 gccaaaaagc cgaaaggccg acgcttcaag gggagaagag cgtcggcctt cctcaaactc
     2281 gcctgcgggg gacgagttta tctgccggcg gccacgtccg cccggcgggc gaagttggcg
     2341 gcggccgtct caagaagcgc gggcggcacg cggcggaagc cgacgatgcg cttggcccag
     2401 tagtcattga aagtcgagta gacgacgccg cggctcgaag aggcgtggta gaagccgccc
     2461 gcgtcggcga cgatgccgat gtggccaagg tggttgaaga agacgagcgt cccgaacttg
     2521 tacttctcct cctccgtggg gaccgcaaac cgcgaccaga acgtgcgcgc gctcccgcgc
     2581 tcgaagttga cccccacctc ctgaaacacg ctccacacaa atcccgagca gtcatagcga
     2641 ttcggcccct cggagccgag gcggtagggc gtgccgaggc ggccctcaat cgcatcgagg
     2701 atggccatgt tgagaccgga gagtcgaagc cccggcgcaa cggtcgtcgc ggcgggcggc
     2761 tcggcctcgt cggtcgtcgc gagcgagatg accatcggct cattttcgag ccgcgtcgcg
     2821 ccgctcgtct cggtgctggt ggtggtggcg gcgatgcgcg ggcgtgcttc ctgcgcgaag
     2881 gcggtcgtgg cgagcaaacc tagaagcgcg catgccgcac acatgcgggg gaacgattta
     2941 aacaaagaca taaccggggg aagtacttct cctcttcctc aaagctttcg gctgaattac
     3001 tttagggaac tgcttgatgt ggggctgaat agtcgcgcga gggcgacgtg cgagaatcat
     3061 cctacaggcg tgttacagac cccacaagcg gcaatgtgac tgtttcgtga tactccaaac
     3121 aggtcgatgt agtttgaccg gaaagagtcc gtcatcaaga cggcaagtgg ctcacattga
     3181 atgaattgtg ggaagtcggt acacattcag cgactcttca gacgagattc cgctgacatt
     3241 caaaggatgg cctcaaaggc acatagcatt ttgcgcgcgc ggcgtgatag cattcgcggc
     3301 caagaagcgg ttgtaaggag caacgttcat gctttctgtc aaaagcaggg ttaccggtcg
     3361 gagggtcgat gcggcggccc gcacgcgtgt ccttgagagg cttcaggaga cgggcttcac
     3421 gccgctcatt cgcacgatgc ggctcggcgc cttcgaggag gcgcttgcgg cgggcggcga
     3481 accccccaaa gatatggaac ggctgatggc ggcctggact gacgccgagt ctgacgcgcc
     3541 ctacgtccga ggcgacctct tctgcttcga gtctcacatc ttcttcctca tcttcgccga
     3601 cgacgcggcg tccgacgatg ccggcctgcg cgcaggcatc attcatgacg ccgaggcgga
     3661 cgcgctcgaa cgccagctcg aatctttctg ccgtaacatc gatgagtccg tggacgcgga
     3721 gtcggcgcgt ggcggcgagg gcgtgggggg cgtcgagtct ggcgcgaacg gcggcgttgc
     3781 gcagggcgac gcggagtggc gcgcgcaaga ggtaagtgcg cccgagagtt tcacacgttt
     3841 cgtcgcgtcg atgtctgagg gcggggccgc ggggcttgcg ccgccacaat ccgcgcgcgg
     3901 ggagacggcg gagcgtctgc gcgcggtgga gatactggaa gacgcggagg cgcgacgcct
     3961 gctgcgtcgc ctcagcgacg cgcaggccga ggggcgcacc gccgagatgt tgctcggcgc
     4021 ggggcgcgag ggcgagtcat tgccggaggg gctggtgcgg cgtctggcgg gcgccgcgct
     4081 tgtgcgccgc gaactcctca tcagttgccg caaggacggg cgctcgctct tccgtctgcc
     4141 gtcggcggac gcgctggccg tgatgacggc gagccacgcc atgtgcagcg agtgcggcgc
     4201 ggccgtcgcc gacgagcgcg ccgaggagtt ggtcacgccg acgccgctcg cggggtcgat
     4261 gctcaaggac ggcgcgtggc tcgtcagccg gatgcgcgcg gcgctcgctg agttgggagt
     4321 cgctgaaact gatgtcgcca cgcgccccgc gacgaacgac tcggacgcgc tgatgatggc
     4381 caacgccgcc ggcgagtcgt tcctcttcgt cctgcgcgac ggcgacttca cgctcgcgca
     4441 cgcgcggcgg gccatcgacg tggaggccga cacggaggtg tcgcacctcg tcgtggtggc
     4501 gacggggaag gtgcaggagg acgcgcgctc acgcctgcgc gaacaccaga agcggcgagc
     4561 gcgcacgggc gccgagcttg acctgctcgc cgtcgagggc gtggacgcgg cggccggcga
     4621 gttgcggcag gcgctcgaac gcgtgacgca gaagtctctg gcgggcgagc tgtacgagtt
     4681 ggacgcgggc ttcggaatga acgccggcgc cttcgtcgcc gcgtgcttcc gactgacgca
     4741 gaagcccgtg gcgttacaag accgcgcggc ctcggcggcg ggcgcgctcg ccgggagcct
     4801 gcgcgagatt taggcgctca actacacgca gacttttacc tgacctcgct cactcaaacc
     4861 gtatcctcgc ttcccgtgcg cgccgtgcgc aacggtgtgg ttatccacgt aatccgccgc
     4921 gagcgcgcgg cgcatcctgt gcgcggccgt gaaggcggcg caagccagtc gggcgacggg
     4981 cgttcacgtg ttggcataag gaggtgccac aacagatgag cagcgagaga aacggacaag
     5041 ttttcaacgc ggggttcgcg ctggcggtgg ggctggtgct ggcctcaatc gtcttcgcct
     5101 gggcttacac ccgcaggcag ggcgccgacc agacgatcac cgtcaccggc tcggccaagc
     5161 ggcgcatcaa gtcagacctc gtcgtctggc gcgccaacat ctcttatcag tcggcgcagc
     5221 tcgcggaggc ctacggcgcg ctcaaggaca gggtgccgcg cgtcaaggcg tacctcgtct
     5281 cgaagggcgt ccccgaggat cggatcgtcg tctcgtcgat ttcgaccaag acgatgcagg
     5341 acaaggacga gggcggcgag gagacgggac agattaacgg ctacgtcctg tcccagaccc
     5401 ttgaggtgcg ctcggacgac atggagaaga tcacgaagat ttcgcgtgag gtgacggagc
     5461 tgatcgacca gggcatcctg ctcgaatcgc aggccccgga gtatcactac acgaagttga
     5521 gcgatctgaa gatcgagatg ctgggcgagg cggcgaagga cgcgaaggtg cgcgcgcagc
     5581 agatcgcttc gagcacgggg agcgaggtgg ggagcgtccg ctcggcccgc atgggcgtga
     5641 tgcagattac ggcggccgac tccaacgagg tctccgacta cggcgtcaac gacacgtcct
     5701 cgctggagaa ggacatcgcg gcggtcgtca acgtcagctt cgccgtggac tgaaaagccg
     5761 tcaacgacga gcgaatttaa ggggcgcgcc gtctcacaag cggcgcgccc ctttgtgtct
     5821 ctgctcacgg acgggacagt accgcgagcg gtagcgagcg ggtctacttg ggtatgaaga
     5881 cccgctcgct accg
//
LOCUS       DASVES010000392        16029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_314698, whole genome shotgun sequence.
ACCESSION   DASVES010000392 DASVES010000000
VERSION     DASVES010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16029
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314698"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VF240_15995"
     CDS             complement(<1..410)
                     /locus_tag="VF240_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176767.1"
                     /translation="MSNHARPFLLVIACCCAALSANMRFGTSAQTQSTRRVTFNTHES
                     LSLNPTLSGDGRRVVFETTAAPAGGVPGFRTVSATVFAHGFTLRELAASRAPAPAVSQ
                     DGSRVAFSSMSDPAGRNPDGNPEIFLHDGLSLTQL"
     gene            complement(710..1000)
                     /locus_tag="VF240_16000"
     CDS             complement(710..1000)
                     /locus_tag="VF240_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176768.1"
                     /translation="MKTGRTLLLLAALAAAHYPAPVVAQQPQVRRYKIYCVEGAVRVE
                     KLTMDELREEFKGPVCELARTGFESMSVARRAAKRFGGVGAPCRCAEARKAA"
     gene            complement(1047..3791)
                     /locus_tag="VF240_16005"
     CDS             complement(1047..3791)
                     /locus_tag="VF240_16005"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7176769.1"
                     /translation="MKFRHRPTCFAAVLLAASLLASATASSQRRATSAGGRPQPEAFV
                     IRVGVISYNSYASGRRQYSNELRAAAAAYKGENVVFQLAVGSYSDVLDWYNKELVDIA
                     FLTPQPVAELRSSLGDEELSRRYLATHASNGGDGVTTPAFEYHSSCYVGKSTPIENVE
                     QLREKARQGRLSFLFVDPLSLSGNIFPRHLLRELGVTNIADNPTDAGLNSATFTYGSK
                     ASIDMLAEAEEIESLFVEDREGEPLENFVEERKWPVAFFSAPARAPEGLKKIDFPALD
                     TPKLPEEVVLATPSFLQHRDKLLRILRVDQPRAFFRSQPYDVSWLKRFDGVSKWVTDL
                     GVKASDPHNRMFTLERIGRILRNYGAERQRPARLALVLSGGGAKCAYQIGAIAAIEAM
                     VREDDTETEAAAEAVAEADGAKKESNIDIDLVVGTSGGAINALAVALGLTRDLPEIQL
                     LEEDPLVKTWKGFEQKDFFSPMPAAYYLLALTTALLQAVLLAWLFVVVVIVGRLIGRD
                     LTWLYRFVGPAMIALAAADALAQLSGARVDTRGGHFWQHVSLLFTFNLYLTAACLLVL
                     GLLLTALLLRRENYLLWFRVTHTLMRNAFLFVVCALIAYSLFRAETLSRADTVESTLA
                     KKLTALVSPKASARLAAGGEADTAQLFSKISNSDLKPMLQRDLVITGSVLDGGQAKAA
                     GTHLHASLPPDIYFFHDKPKCGDEKGTPACDPDAPRDAQGLFRDFDPKNGRNYLLDVV
                     IGSSSIYPIFPARKLADYGQIVDGGFAHNSPVEAAVMWKATHIILVEASPTPTTSESH
                     LWQNAQNALNHLYIQAQLSDLRAHGKVEMFILRPEETPEEPWMCTFDFNSDSISHAIR
                     RGYDDAGMGTKPRFERVHGEPVFELVDYRNTADAVDADNQCVCPEKPAANGEAVPNID
                     "
     gene            complement(4074..5984)
                     /locus_tag="VF240_16010"
     CDS             complement(4074..5984)
                     /locus_tag="VF240_16010"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX7176770.1"
                     /translation="MKDFREHIEGDHITVFDGAMGTMLYAKGVYINRSYDELNLTNPD
                     LVREVHQEYIRAGAEVVETNTFGASAHKLQQYGLEGSMRLINVAAARLAREAAGDRAF
                     VAGAVGPLGLRIEPYGPTSFEEAKELFKHQAEALLEGGVDLFLLETFSDISEMRQAIR
                     AVRELCDLPVVAQMTVQNDGSTSFGTTPEIFTSRLDEWGADVIGLNCGVGPAILLSAV
                     EKMRAVTTRKLSAQPNAGFPRDVQGRQFYMCSPEYMAKYAKRLIQAGVKFVGGCCGTT
                     PAHIKLISDAVRAVSPRIYRGAGALDASSAQPARIEELTPADIRVVPPEERSNWARRL
                     ARGEFVTTVEVLPPKGCDPDKTLDSIRLLKEAGVQAVNIPDGPRAQTRMSAQATALLV
                     EREIGIESVLHYCCRDRNLLGMMSDLLGAAALGLRNLLLITGDPPKMGPYPDATAVFD
                     IDSIGLTNMVNKLNHGLDLGNNPIGCPTSFCIGVGVNPGSLNLDEEIKRFEWKVEAGA
                     EFAITQPVFDTEQLCDFLRRIEHVRIPVVAGIWPLVSYRNAEFLHNEVPGVRVTPSIM
                     ERMRVASERGKEAARDEGLQIARELLTEVRDRVQGVQVSAPFNNVRYALEVFDALPEF
                     AARARPEKVAGG"
     gene            6315..6824
                     /locus_tag="VF240_16015"
     CDS             6315..6824
                     /locus_tag="VF240_16015"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7176771.1"
                     /translation="MSISQSLLPEFKQEMANTRKTLERVPDDKFDWKPHEKSTTMGDL
                     ATHLANILTWALHGLNKDSIDIAPPGEAPLRAKPAATRDEVLETFDKNLADALAAIAA
                     ATDERLMQPWTLLHGGKTVFTLPRVAMLRGFVMNHTIHHRAQLGVYLRLNDIPVPSIY
                     GPSADEGGF"
     gene            6969..7736
                     /locus_tag="VF240_16020"
     CDS             6969..7736
                     /locus_tag="VF240_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7176772.1"
                     /translation="MNLFGYRTAAQRYTKGRPYFHPKVIARVHDFLGLTGPVEAALDV
                     GCGSGLSAVALAEIARRVVGVDASHEMLALAPPDARVSYAAAPAEELPFGDGAFDLLT
                     ASQAFHWFDRHGFLKEARRVLRPGGHLVVYDHYFAARMEENEAFRVWFREQYLGKYPS
                     PKRGDIVFTESEAGEYGFRLLGVEYYDDALKFTQESLIDYLTTHSNIIAAVEGGGGES
                     IDEARAWLAESAGPLFGGMDEATFLFRGPIWYLRRGD"
     gene            complement(7755..8588)
                     /gene="mutM"
                     /locus_tag="VF240_16025"
     CDS             complement(7755..8588)
                     /gene="mutM"
                     /locus_tag="VF240_16025"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX7176773.1"
                     /translation="MPELPEVELVARALDHHARGRRITAARLLRPGLAPDIAPRTFAR
                     LLRGALVESVTRRGKHLLFQLDCGRVLITHLRMTGRFLLLPQHRPLPPHTHALFQLDD
                     GRRLVFSDQRHFGMMKLVSTDELHLARELQHLAPEPFSDDFDDRYLRGVLAGSRRTLK
                     ATLLDQTKVTGLGNIYAAEVLFLARVNPFLASSDLSARRVPRLRRSILEVLSESIAHG
                     STMNVDPENIDGSYYGGAYEGRWRVYDREGEPCLDCRASVRRLSHAGRSTYFCPRCQR
                     R"
     gene            complement(8699..10492)
                     /locus_tag="VF240_16030"
     CDS             complement(8699..10492)
                     /locus_tag="VF240_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176774.1"
                     /translation="MPPPYDTVISIGGQSLVDGVWRLLGPALEGLSNPLPLALPGFSN
                     ASMRVTRLTPVFPGTPPPTGGAALEVLVNVELNAEALLNVNVATESISISLGPQQLNL
                     TSLTGTIDLPTLTGNLTNLNISGNTNPVGSSTTLLNDIDLGPGTGTLNLPASTANLTN
                     GNLTGTLNLTLPRVPLPAVVPVAVDLTPSAPLVVSALLQLTVRGIDPTTRFGLLIEVG
                     NPSVGAVALDPALAAMLTTSLQSAVNQIVQQLGIPNVITQSPVNAATVSALLAPLANV
                     VRAALTDALRSLLAATGRLIYPPAGAGASCDVQVLPTVADASLAVAGGAYVLQIGFRR
                     PSSTDIATLPPFAPAGIADSTLLVGNRFLLELLCCLVQRLPGFTFPVAASNATTDVTG
                     ASHLMCCNLNGATVNLGPVALNGGLSVCIDGSSGSPKSITLVGGFGQGAPVGSLPLAT
                     IDVRFTLPLTFDLDDVASLANLRVSGSPAVSVAVSPNPWLVFIIAIGGLLLIGPAVAV
                     VANALLLASCIMASRLLDSAVRTVLGAASLVRSPVSIPPGVFEAFGKLVPVTVTIDDL
                     TAHSVLQTPTSPWAVLPRIGRDRPSKPDSVD"
     gene            complement(10588..12030)
                     /locus_tag="VF240_16035"
     CDS             complement(10588..12030)
                     /locus_tag="VF240_16035"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEX7176775.1"
                     /translation="MTSRAEQRTTIRFGTDGWRGVIASEFTFANVARVAQAYADYLLS
                     QRPIESDRPRTPPHVIVGFDRRFLSEQFASTATEVLAGNGVRISSFDADVPTPLVSWA
                     VRERGAQGGVVITASHNSATFNGFKIKAPFGGSATPEMTVAVEALMDANSPRRGEASA
                     GDDLTSSVESYREQVASYVDLERLRGARAHVVVDPMHGTGGQWVESFLGGGQLTAETI
                     RGERDPLFGGVAPEPIDRNLAPLKRRVKETGALVGLATDGDADRVGAVNEAGETLTMH
                     EVVPLILLHLARARRMTGGVVRTFSQSVLLRRIAAAEGLKIFETPIGFKYIADLMLRE
                     DILIGAEESGGIGVRGHLPERDGILNSLLFLETIVAAGKTPTALVREMHREFGEFYYD
                     RRDLHTDVARGLALVAELATTPPAGVAGFEVAGVERTDGTKLLFKDESWLLFRQSGTE
                     PVLRIYSEATSKEKVRQLLEAGSALAQAVH"
     gene            complement(12027..13076)
                     /locus_tag="VF240_16040"
     CDS             complement(12027..13076)
                     /locus_tag="VF240_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX7176776.1"
                     /translation="MQALILAGGKGTRLRPLTLYTPKPVVPICNRAFLLYQIDTLRRA
                     GIHDITLSLSYQPLKIEQQLGDGTDFGVKINYTVEPQPMGTAGAYKYAEDFIRQPTVV
                     FNGDILTDLDLQAVISEHRERGAMATVVLAPVDNPSAYGLVETESDGRIRAFREKPKA
                     DEISVNTINAGTYVLEPQVLDLIPQGENYSFEYGLFPDLLKRGEKFYAHVPDAAYWID
                     IGTPARYLQSHLDILAGRVRNVETPPLRGDFDAATHAEIDPLSMVADNCTVKPGAQII
                     NSVLGEGVYVEERARVENSVMWAHTRVGAGAQVFGAIVGRGCHIGRAAQLGAGAVLGD
                     KSSLNDYTQTGGGLS"
     gene            13341..14531
                     /locus_tag="VF240_16045"
     CDS             13341..14531
                     /locus_tag="VF240_16045"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7176777.1"
                     /translation="MPDRREAVIIGAARTPTGKFMGALKDFTAPQLGAMVVGEAVRRA
                     GVRPEDVDEVIMGCVIQAGLGQNPARQAALKGGIPFGVSAVTINKVCGSGLKSVMMAA
                     QGIQLGDSEVVVAGGMESMSNAPYLLPKAREGYRMGNAQIVDSMINDGLWCAFENYHM
                     GNTGEVVAERYGVTRAEQDEYAYNSHRKAAAAIKAGKFKDEIVPVEIPQKKGAPVLFD
                     TDETVREDTSVEVLGKLRPAFKKEGGTVTAGNAPGVNDGASALVVTSLERAREMGAEP
                     LARIVAQATSGIQPELVMMAPVEAIRKVVSKAGWSLSEVDLIELNEAFSVQAIAIMRE
                     LELDPARVNVNGGAVALGHAIGQSGSRLLTTMLYEMRRTGARRGLAALCLGGGNAVAL
                     AVER"
     gene            14633..15163
                     /locus_tag="VF240_16050"
     CDS             14633..15163
                     /locus_tag="VF240_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176778.1"
                     /translation="MTPADHNKAIGIMHLIYGGFFTLMTLMMLVFFGFFAALMSSIPQ
                     EPGAPPAAFFSAIFGFIVVIYALLSIPSLVAGYALLKRKSWARVAGIVASILAGVSFP
                     FGTALCVYSLWYFFGEQGRDYEARLAGAHRWPGSLNHAAPSGWSAQQQQQQQQQDAES
                     RQSAYHPPPSPPDWRG"
     gene            15191..>16029
                     /locus_tag="VF240_16055"
     CDS             15191..>16029
                     /locus_tag="VF240_16055"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF004474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX7176779.1"
                     /translation="MSETIGVVGAGTMGNGIAQVAARAGHRVLLRDVAPEFLERGMGA
                     IDRSLQRDVDKERLKAEEKREIVGRIETTTELAALAGADFIVEAVTEKLEVKAEVFRQ
                     LDEIAKPEAILASNTSSISITKLGAATCRADRVIGMHFMNPVPVMKLVEVIRGIATSD
                     DTWERTRALTERLGKTALECHDSPGFVSNRVLMPMINEAIFALHEGVATRESIDGIMK
                     LGMNHPMGPLTLADFIGLDVCLAILNVLHTELGDPKYRACPLLKRYVDAGWLGRKSGR
                     GFY"
BASE COUNT         2727 a         4907 c         5699 g         2696 t
ORIGIN      
        1 agttgtgtga gggagaggcc gtcgtggaga aagatttcgg ggttgccgtc ggggttgcgc
       61 ccggcggggt cgctcatgct gctgaaggcg acgcgcgagc cgtcctgcga gacggcgggc
      121 gcgggcgcgc gcgaggcggc gagttcacgg agagtaaacc catgagcgaa gacggtcgcg
      181 gagactgtgc ggaagccggg cacgccgcct gcgggagcgg cggtcgtctc gaagacgacg
      241 cgccggccgt cgccgctaag cgtggggttg agactgaggc tttcgtgcgt gttgaaggtg
      301 acacgacgcg ttgactgcgt ctgtgcggaa gtgccgaagc gcatgttggc ggacagcgcc
      361 gcgcagcagc aggcgatgac gagcagaaag ggccgtgcgt gattagacaa ggcttcgctc
      421 ccgtgtgagg tgcgcccaca ttgtgtgcgg acgcgttgag gaaacgagct gccacaggcg
      481 cgacggcgca acgtgagggg ccaacgagcc tgggccttcg gacgtaaacg aaatcgacgc
      541 tgcgaaaata gtcgggcgcg ttaaggcttc ggcgggtgcc gagcgtgatg aggaaatacg
      601 cccttcggac gaatgcgacg ccgggcattc tacaactttc gccggcgagc gcaagagaaa
      661 actggcgcga agggtgcgcg ccgtggcggg aggccgcaaa cgggcggcgt caggcggcct
      721 tgcgtgcctc ggcgcaacgg cagggcgcgc cgacgccccc gaagcgcttg gcggcccgac
      781 gcgcgaccga catcgactcg aagccggtgc gcgccagctc gcagaccggc cctttaaact
      841 cctcgcgcag ctcatccatc gtcagcttct cgacgcgcac cgcgccctcg acgcagtaaa
      901 tcttatacct gcgcacctgc ggctgctgcg cgacgactgg ggcggggtag tgtgcggcgg
      961 cgagggcggc gagtagcagg agcgtgcgtc ccgttttcat ggtcggttcc tccgtggcgg
     1021 gcggcggccg gcgcgcgcgc gtccgtctaa tcgatgttgg ggacggcttc gccgttggcc
     1081 gcgggttttt cggggcagac gcattggttg tcggcgtcca cggcatcggc ggtgttgcgg
     1141 tagtccacca gctcgaagac cggctcgccg tggacacgct cgaagcgggg cttggtgccc
     1201 atgccggcgt cgtcgtagcc gcggcggatg gcgtggctga tggagtcgga gttgaagtcg
     1261 aaggtacaca tccacggctc ctcgggggtc tcctcggggc ggaggatgaa catctcgacc
     1321 ttgccgtggg cgcgcaggtc tgagagctgc gcctgtatgt agaggtggtt gagggcgttc
     1381 tgcgcgttct gccagaggtg cgactcgctc gtcgtcgggg tcggcgacgc ctcgacgagg
     1441 atgatgtgcg tggccttcca catcacggcc gcctcgacgg ggctgttgtg ggcgaaaccg
     1501 ccgtccacga tctggccgta atcggccagc ttgcgcgcgg ggaagatggg gtagatggag
     1561 cttgagccga tgaccacgtc gagcaggtag tttctcccgt tcttagggtc gaagtcgcgg
     1621 aagaggccct gcgcgtcgcg gggcgcgtcg gggtcgcacg ccggcgtccc cttctcgtcg
     1681 ccgcacttcg gcttgtcgtg gaagaagtag atgtcgggcg ggagcgacgc gtggaggtgc
     1741 gtgccggcgg ctttggcctg ccctccgtcg agcaccgagc cggtgatgac gaggtcgcgc
     1801 tggagcatcg gcttgagatc gctgttggaa atcttcgaga acagttgggc cgtgtcggcc
     1861 tcgccgcccg ccgccagccg ggccgacgcc ttggggctga cgagcgccgt gagtttcttc
     1921 gccagcgtgc tctcgacggt gtcggcacgc gagagcgtct cggcccggaa gagcgagtag
     1981 gcgatgaggg cgcagacgac gaacaggaag gcgttgcgca tcagcgtgtg ggtgacgcgg
     2041 aaccacagca gatagttctc gcggcgcagg agcagggccg tcagcagcag gccgaggacg
     2101 aggagacagg cggccgtcag gtagaggttg aaggtgaaga gcagggagac gtgttgccag
     2161 aagtgcccgc cgcgtgtgtc cacgcgcgcc ccggacagtt gcgcgagcgc gtcggcggcg
     2221 gccagcgcga tcatggcggg gccgacgaag cggtagagcc acgtcaggtc gcggccgatc
     2281 agcctgccga cgatgacgac gacgacgaag agccacgcga ggagcacggc ctgaagcagt
     2341 gcggtcgtta gcgctagcag atagtaggcc gccggcatcg ggctgaaaaa gtccttctgc
     2401 tcgaacccct tccaggtctt caccagcggg tcttcttcga gcagttggat ttccggcagg
     2461 tctctcgtca gaccgagcgc gacggcgagg gcgttgatgg ccccaccgga ggtgccgacg
     2521 acgaggtcga tgtcaatatt cgactccttc ttcgcgccgt cggcctcagc cacggcttcg
     2581 gccgcggcct cggtttccgt atcgtcctcc ctgaccatcg cctcgatggc ggcgatggcg
     2641 ccaatctggt aggcgcactt ggccccgccg cccgagagca cgagggcgag acgcgccggg
     2701 cgctggcgct cggccccgta gttgcgcagg attctcccga tgcgttcgag cgtgaacatg
     2761 cggttgtgcg ggtcgctcgc cttgaccccg aggtctgtga cccacttcga cacgccgtcg
     2821 aaccgcttga gccaactcac gtcgtaaggc tgcgacctga aaaatgcgcg gggctgatcg
     2881 acgcggagga tgcgcaggag cttgtcgcgg tgttggagga aggagggcgt ggcgagcacc
     2941 acctcctcgg gcagcttcgg cgtgtccaga gcggggaagt caatcttctt cagtccctcg
     3001 ggcgcccgcg ccggggccga gaagaaggcc accggccact ttctctcctc cacgaagttt
     3061 tcgagcggct cgccttcgcg gtcttcgacg aagagagact caatctcctc ggcctcggcg
     3121 agcatgtcga tggaagcctt gctgccgtag gtgaacgtcg cggagttgag gcccgcgtcc
     3181 gtcgggttgt cggcgatgtt cgtgacgccc agctcgcgca gcaggtgccg ggggaagatg
     3241 ttgccggaga gcgagagcgg atcgacgaag aggaaggaga ggcggccctg ccgggccttc
     3301 tcccgcaact gctcgacgtt ttcgatgggc gtgctcttcc cgacgtagca ggagctgtga
     3361 tactcgaagg cgggcgtggt gacgccgtcg ccgccgttgc tggcgtgcgt ggcgagatag
     3421 cggcggctca gctcctcgtc accgagcgac gagcgcagct cggccacggg ctgcggcgtc
     3481 aggaaggcga tgtcaaccag ctccttgttg taccagtcga gcacgtcgct gtaggagccg
     3541 acggcgagct ggaagacgac gttctcgccc ttgtaggcgg cagcggcggc gcgcagctcg
     3601 ttgctgtatt gccggcggcc gctggcgtag gagttgtagg agatgacacc gacccggatg
     3661 acgaacgcct cgggctgtgg gcggccgccg gcggaggtcg cgcggcgttg cgagctggcc
     3721 gtggcggagg ccagaaggga ggcggcgaga agaacggcgg cgaaacaggt gggacgatgc
     3781 ctgaacttca tgtggcacct ctgctagtcg gagatgtggg gcgcgctttg ggacttgggc
     3841 gcgcttgtga gcagcggggc agacgcctca ggtcaggaga ggctcaaaga cgctttccaa
     3901 gggccggccg gcagcgattc tacaacttcc ggcgcgtaaa gcaacaggaa atcggaagac
     3961 gcgttcgacg gccctcgccc cggcttgggg cttgggccgc acgcctcacc cgtctgtata
     4021 tgcgtccggc gtcgaagtcg tttcgcggcg gccgctccgc cgcgcctcgc gtctcagccg
     4081 cccgcgacct tctcgggccg ggcgcgggcg gcgaactcgg gcagcgcgtc gaagacttcg
     4141 agggcgtagc ggacgttgtt gaagggtgcg gagacctgca cgccctgcac gcggtcgcgc
     4201 acctccgtga gcagctcgcg ggcgatctgt aagccttcgt cgcgcgcggc ctctttgccg
     4261 cgctccgagg cgactctcat gcgctccatg atcgagggcg tgacgcgcac gcccggcact
     4321 tcgttgtgca ggaactcggc gttgcggtag gagacgagcg gccagatgcc ggcgacgacg
     4381 gggatgcgga cgtgctcgat gcggcggagg aaatcgcaga gctgttcggt gtcgaagacg
     4441 ggctgcgtga tggcgaactc cgccccggcc tccaccttcc actcgaaacg cttgatctct
     4501 tcgtcgaggt tgagcgagcc ggggttgacg cccacgccga tgcagaagct cgtcgggcag
     4561 ccgatggggt tgttgccgag gtcgaggccg tggttgagct tgttgaccat gttggtcagg
     4621 ccgatggagt cgatgtcgaa gacggccgtg gcgtccgggt aaggccccat cttcggcgga
     4681 tcgccggtga tgagcaggag gttgcgaagg cccagtgcgg cggcgccgag caagtcgctc
     4741 atcatgccga gcaggttgcg gtcgcggcag cagtagtgga gcacggactc gatgccgatc
     4801 tcgcgctcga cgagaagcgc ggtggcctgc gcgctcatgc gcgtctgcgc gcgcggcccg
     4861 tcgggaatgt tgacggcctg cacgcccgcc tctttgagca gtcgaatcga gtcgagcgtc
     4921 ttatcggggt cgcagccctt tggcggcagc acctcgacgg tcgtgacgaa ctcgccgcgc
     4981 gcgagccgtc gcgcccagtt cgagcgctct tcgggtggga cgacgcggat gtcggccggc
     5041 gtcaactctt cgatacgcgc cggttgtgcc gacgacgcat ccagcgcgcc ggcgccgcgg
     5101 tagatgcgcg gggagacggc gcgcacggcg tcggagatga gcttgatgtg cgccggcgtc
     5161 gtcccgcagc agccgccgac gaacttcact cccgcctgaa tgagccgctt ggcgtacttg
     5221 gccatgtact cgggcgagca catgtagaac tgccggccct gcacgtcgcg cgggaaaccg
     5281 gcgttgggct gcgccgagag cttgcgcgtg gtgacggcgc gcatcttctc gacggctgag
     5341 agcaggatgg ccgggccgac gccgcagttg agaccgatca cgtcggcgcc ccactcgtcg
     5401 aggcgcgaag tgaagatttc cggtgtcgtg ccgaaagagg tgctgccgtc gttctggaca
     5461 gtcatctgcg cgacgacggg caggtcgcac aactcgcgca cggcgcggat ggcctggcgc
     5521 atctcggaga tgtcggagaa cgtctccagc aggaacaaat cgacgccgcc ttcgagcaga
     5581 gcctcggcct gatgcttgaa cagctccttc gcttcctcga acgaggtcgg gccgtatggc
     5641 tcgatacgca ggccgagcgg gccgaccgct cccgcgacga aggctcggtc gccggccgcc
     5701 tcgcgggcga gacgcgccgc cgcgacgttg atgaggcgca tgctcccttc cagcccgtac
     5761 tgctggagct tgtgggccga tgcgccaaag gtgttcgtct cgacgacctc ggcgccggcg
     5821 cggatgtatt cctgatgcac ctcgcgcacg aggtcggggt ttgtcaggtt cagctcgtcg
     5881 taggagcgat tgatgtagac gcccttcgca tagagcatcg tccccatcgc cccgtcgaag
     5941 acggtgatgt ggtcgccctc gatgtgttct ctgaagtctt tcataaggca ggagtcagaa
     6001 gtcaggagtc agaagagaag cagtcaggtt attcattctg actcctgact tctgactcct
     6061 gcctcctcaa aaaaaatatc ccgcgctaaa caacgcggga cttcacgccg gaacctgaag
     6121 tttgcttgaa gcgcctcggg aagcgccgtg ctgtttagcg gttattttac gtggccgcaa
     6181 gtcgccttaa ctcaccacgt ttttccgaag ccgcttatac gccgcgcgcc gccggcttgt
     6241 caagcaacgc ttctgtttgc aacccccgcc cgtctgcggt aaaagaccaa catcaaaccc
     6301 cccggaggtc aacaatgagc atcagccaat cgcttctgcc cgagttcaaa caggagatgg
     6361 ccaacacccg caagacgctc gaacgcgtcc ccgatgacaa gttcgactgg aagccgcacg
     6421 agaagtccac gacgatgggc gacctcgcca cgcaccttgc aaacatcctc acgtgggcgc
     6481 tccacggatt gaacaaagac tccatcgaca tcgccccgcc gggcgaggcg ccgctgcggg
     6541 ccaagccggc cgcaacacgc gacgaggtgt tagagacgtt cgacaaaaat ctcgccgacg
     6601 cgctcgcggc catcgccgcg gcgacggacg agcgcctcat gcagccgtgg acgcttctgc
     6661 acggagggaa gactgtcttc accctgccgc gcgtggccat gctgcgcggc ttcgtcatga
     6721 accacaccat ccaccaccgc gcgcagctcg gcgtctacct gcggctgaac gacatccccg
     6781 tcccgtccat ctacggccca tcggccgacg agggcggctt ctagtaggac aggcattcct
     6841 gcctgtcgtg agcgcgaaag cgcgaataaa cttcgcgcgc acattctaac ttgagattcg
     6901 gcgcatcacc tgttcgcgct ggcgcgctca cgacaggcag gaatgcctgt cctacttcaa
     6961 atgccgcgat gaacttgttc ggctacagga ctgcggcgca gagatacacg aagggtcggc
     7021 cttacttcca cccgaaggtc atcgcgcgcg tccatgattt tctcggactc acggggccgg
     7081 tcgaggccgc gctcgacgtg ggctgcggct cgggtctctc ggccgtggcc ctcgcggaga
     7141 ttgcgcggag agtcgtcggc gttgacgctt cccatgagat gctcgcgctc gcgccgccgg
     7201 acgcgcgcgt ctcctacgcg gccgcgccgg ccgaagagtt gcccttcggc gacggcgcgt
     7261 ttgatctgct gacggcctcg caggctttcc actggttcga ccgccatggc tttctcaaag
     7321 aggctcgcag ggtcttacgg cccggcgggc atctggtcgt ctacgaccac tacttcgccg
     7381 cgcggatgga ggagaacgaa gcgttccgcg tgtggttccg cgagcagtat cttggaaaat
     7441 acccaagccc gaagaggggc gacatcgtct tcacagagtc ggaggcgggc gaatacggct
     7501 tccgcctcct cggcgtcgaa tactacgacg acgctttgaa gttcacgcag gagagtttga
     7561 tcgactacct cacgacacac agcaacatca tcgcggccgt cgagggcggc ggcggcgaga
     7621 gcatcgacga ggcgcgggcg tggctggccg aaagcgcagg gccgttgttc ggggggatgg
     7681 acgaggcgac gtttcttttc cgggggccga tctggtattt gcggcggggc gactgagcgc
     7741 gtttcacggc cgggttaacg ccgctggcag cgcgggcaga agtaggtgga gcggccggcg
     7801 tgtgagaggc ggcggacgct cgcgcggcag tcgaggcaag gctcgccctc gcggtcgtag
     7861 acgcgccagc ggccttcgta agcgccgccg taatagctgc cgtcgatgtt ctcgggatcg
     7921 acgttcatcg tcgagccgtg cgcgattgac tcggagagga cttcgaggat cgagcggcgc
     7981 aggcgcggga cacggcgcgc gctgaggtcg gacgacgcga ggaagggatt cacgcgggcg
     8041 aggaaaagga cttcggcggc gtagatgttg ccgaggccgg tgaccttcgt ctggtccaga
     8101 agcgtcgcct tcagcgtgcg gcgcgagccg gcgagcacgc cgcgcaggta acggtcgtcg
     8161 aagtcgtcgg agaaaggctc gggcgcgagg tgttggagtt ccctcgcgag atgaagctcg
     8221 tcggtcgaga cgagtttcat catgccgaag tggcgctggt cggagaagac gaggcggcgg
     8281 ccgtcgtcga gttgaaagag ggcgtgggtg tgtggcggga gcggcctgtg ctgagggagc
     8341 aggaggaagc ggccggtcat gcgcaggtgc gtgatgagca cgcgcccgca gtcgagttgg
     8401 aagagtaggt gcttgccgcg ccgtgtgacc gactcgacga gcgcgccgcg caggagccgc
     8461 gcgaacgtgc gcggggcgat gtcgggcgcg aggcccggcc gcaggagccg cgccgcggtg
     8521 atgcgccggc cgcgcgcgtg gtggtcgaga gcgcgtgcga cgagttcgac ctcgggtagt
     8581 tcgggcatct gtccggtctt cagggtctcc gcgctcagtt tgtggttttt actaaagcca
     8641 gcacacgaac gtcagagttc aggtgggcgc gctgtccggc tccgggtcga tccagtcatc
     8701 agtcaacact atcgggcttg cttggacggt cgcggccgat gcgcggcagc acggcccacg
     8761 gcgatgtcgg ggtctgtaat acgctgtgcg ccgtgaggtc gtcgatggtg accgtcaccg
     8821 gcaccagctt gccgaaggcc tcaaacaccc cgggcgggat cgagacgggc gagcggacga
     8881 gcgacgccgc gcccaggacc gtgcggacgg cgctgtcgag caaccttgag gccattatgc
     8941 aggaagcgag cagcagggcg ttggcgacga ccgcaaccgc ggggccgatg agcaggaggc
     9001 cgcctatcgc gataatgaat accaaccaag ggttgggact gaccgccacc gatactgccg
     9061 gcgacccgga cacacgcagg ttggccagcg acgccacgtc gtcgaggtcg aaagtgagag
     9121 gcagagtgaa tctgacgtcg attgttgcga gcggaaggga gccgacagga gcgccttgcc
     9181 cgaagccgcc gacaagtgta atcgacttcg ggctgccgct cgacccgtcg atgcacaccg
     9241 agagaccgcc attgagcgcg accgggccga ggttgaccgt ggcgccgttg aggttgcaac
     9301 acatcaggtg cgacgcgcca gtgacgtcgg tggtcgcgtt cgacgcagcg accgggaacg
     9361 tgaacccggg caatcgctgg acgaggcagc acaggagttc gaggaggaac ctgttgccga
     9421 cgagcagggt gctgtcggcg atgccggcgg gcgcgaacgg cgggagcgtc gcgatgtcgg
     9481 tgctgctcgg gcggcggaag ccgatttgca gcacatacgc gcctcccgcg acggccagag
     9541 acgcgtcggc gacggtgggc agaacctgta cgtcgcacga cgcgccggcg cccgcgggcg
     9601 ggtagatcag acggccggtc gcggcgagca ggctcctgag cgcatccgtg agcgccgcgc
     9661 ggacgacgtt ggcgagtggc gcgagcaacg cggacaccgt cgccgcgttg accgggcttt
     9721 gcgtaatcac gttcggaatg ccgagctgct gcacgatctg gttgacggcg ctctggagac
     9781 tcgtcgtgag catggcggcg agtgccggat cgagcgcgac ggcgccgacg ctggggttgc
     9841 cgacttcgat gagcagaccg aaacgcgtcg tcgggtcgat gcccctgacg gtaagttgga
     9901 gcagcgcgct caccacgagt ggcgcggaag gcgtgaggtc aaccgcgacg ggcacgacgg
     9961 ccgggagcgg aacgcgcggc agcgtcaggt tcagcgtgcc ggtgaggttg ccattggtga
    10021 ggtttgccgt gctcgccggc aaattgagcg tgcccgtccc cggcccgaga tcgatatcgt
    10081 tgaggagtgt ggtgctgctg ccgacggggt tggtgttgcc gctgatgttg aggttggtca
    10141 gattaccggt cagggtgggc aggtcaatcg tccccgtcag gctcgtcagg ttcagttgtt
    10201 gcgggccgag cgagatgctg atgctttcgg tagcgacatt gacgttcagt agcgcctccg
    10261 cgttcagctc gacattgacg agcacttcga gcgcggcgcc acccgttggt ggcggcgtcc
    10321 ccggaaagac tggagtgagg cgcgtaacgc gcatcgacgc gttgctaaag cccggcaacg
    10381 cgagcggcaa agggttggat aacccttcga gcgccggccc gagcaggcgc cagacgccat
    10441 cgacgagcga ctgcccgccg atactgatga ctgtatcgta tggtgggggc atgtgggtct
    10501 ccgtaagtct cgaactgttg tttcaaaaaa cgcgcgcgcc cggcatctgc ggtgagtata
    10561 acgcgccata cgttcgggtg aaacaaatca atgaacggct tgagcgagcg ccgagccggc
    10621 ctccaaaagc tgtcgcacct tctccttcga cgtggcctcg gaataaatcc tcaagaccgg
    10681 ctccgtgccc gactgccgga agagtagcca cgattcatcc ttgaagagaa gcttcgtccc
    10741 gtccgtgcgc tccacgcccg cgacctcgaa cccggccacc ccggcgggcg gagtcgtggc
    10801 cagctccgcg acgagcgcga ggccacgcgc cacgtccgtg tggaggtcgc gccggtcgta
    10861 gtaaaactct ccgaactcgc ggtgcatctc gcgcaccaac gcggtcggcg tcttgccggc
    10921 ggcgacgatg gtttcgagga agagaagcga gttgagtatg ccgtcgcgct cgggcaggtg
    10981 gccgcgcacg ccgatgccgc ccgactcctc ggcgccgatg aggatgtctt cgcgcagcat
    11041 caggtcggcg atgtacttga agccgatggg ggtctcgaaa attttcaagc cctcggccgc
    11101 cgcgatgcgc cgcagcagca ccgactgcga gaaggttcgc accacgccgc ccgtcatccg
    11161 gcgcgcgcgc gcgaggtgga gcaggatgag cgggacgacc tcgtgcatcg tcagcgtctc
    11221 gcccgcttcg ttcacggcgc cgacgcggtc ggcgtcgccg tccgtggcca gtccgacgag
    11281 cgcgccggtc tccttgacgc ggcgcttgag cggcgcgagg ttgcggtcaa tcggctccgg
    11341 ggccacgccg ccgaagagcg ggtcgcgctc gccccggatg gtctcggccg taagctgccc
    11401 gccccccagg aaactctcga cccactgccc acccgtgccg tgcatcggat cgaccacgac
    11461 gtgcgcgcgc gcgccgcgca gccgctccag atcgacgtag gaggcgacct gttcccgata
    11521 gctttcgact gaagaagtca ggtcgtcgcc cgccgaagcc tctccgcgcc ggggcgagtt
    11581 cgcgtccatt agcgcctcga cggcgacggt catctccggc gtcgcgctcc cgccgaacgg
    11641 ggccttgatc ttgaagccgt tgaaggtcgc cgagttgtgc gaggcggtga tgacgacgcc
    11701 gccctgcgcg ccgcgctcgc gcacggccca cgagacgagc ggggtcggca catcagcgtc
    11761 gaagctggaa atgcggacgc cgttgccggc gaggacttcc gtggcggtgc tcgcaaactg
    11821 ctcggagagg aagcgtcggt cgaagccgac gatgacgtgg ggcggggtgc gcggtcggtc
    11881 ggactcaatg gggcgctgcg acagcaggta gtcggcgtaa gcctgcgcga cgcgcgcgac
    11941 gttggcgaag gtgaactcgc ttgcgatcac gccgcgccag ccgtcggtgc caaacctgat
    12001 ggtggtacgc tgctcggcgc gtgaggtcac gagaggccgc ctcccgtctg cgtgtagtcg
    12061 ttcaatgaag acttgtcgcc gaggacggcg cccgcgccca actgcgccgc gcgcccgatg
    12121 tggcagccgc gcccaacgat ggccccgaag acctgcgcgc ccgctccgac gcgcgtgtgc
    12181 gcccacatca ctgagttttc gacgcgcgcg cgctcctcga cgtagacgcc ctcgccgagc
    12241 accgagttga tgatctgcgc gccgggcttc accgtgcaat tgtcggcgac catcgagagc
    12301 gggtcgatct cggcgtgagt ggccgcgtcg aagtcgccgc gcaggggcgg cgtctcgacg
    12361 ttcctgacgc gtccggcgag gatgtccaga tgcgactgga ggtagcgcgc cggggtgccg
    12421 atgtcgatcc agtaggccgc gtcggggacg tgcgcgtaga acttctcgcc gcgcttcagg
    12481 aggtcgggga agagaccgta ctcgaaggaa tagttctcgc cctgcgggat gaggtcgagc
    12541 acctgcggct ccagaacata ggtgccggcg ttgatggtgt tgacgctgat ctcgtcggcc
    12601 ttgggcttct cccgaaaggc gcggatgcgg ccgtccgact cggtctcgac gagaccgtag
    12661 gcgctcggat tatcgacggg cgcgagcacg accgtggcca tcgccccgcg ctccctgtgc
    12721 tcactgatga cggcttgcag gtcgaggtcg gtgaggatgt cgccgttgaa gacgaccgtc
    12781 ggctggcgga tgaagtcttc ggcgtacttg taagcgccgg ccgtgcccat cggctgcggc
    12841 tcgacggtgt agttgatctt gacgccgaag tcggtgccgt cgccgagctg ctgctcgatc
    12901 ttcaggggct ggtaggagag cgagagcgtg atgtcgtgga tgccggcgcg gcgcagcgtg
    12961 tcgatctggt agagcaggaa cgcgcggttg cagatgggaa cgacgggctt cggggtgtag
    13021 agcgtcagcg gccggagccg cgtccccttg ccgccggcga gtatgagagc ctgcatgttc
    13081 ggcgtaaaac tttcgttgct ggattcgcgt gcgacaggcc gaaggtgagt aggttttaac
    13141 gccttcgccc cggcacgcgc ggcggagtct aacatccgcg attcactgtt tacaaacgac
    13201 cccgcggcga acgcttcctt gacacccctc gcgagccgac atgataatca aaccataaaa
    13261 tcaagcactc gttagattgt ttatgaggat gactccggct cacatgcggc ggtcaatttc
    13321 ggggcaaggg aggagcagtt atgccggaca gaagagaggc cgtcatcatc ggcgcggcgc
    13381 gcacgccgac gggcaagttt atgggcgcgc tcaaggactt tacggcgccg cagctcggcg
    13441 cgatggtcgt cggcgaagct gtacgccgcg ccggcgtgcg gcccgaggac gttgacgagg
    13501 tcatcatggg ctgcgtcatt caggcgggtc tgggtcagaa cccggcgcgg caggcggccc
    13561 tcaagggcgg catcccgttc ggcgtctcgg ccgtgacgat taacaaggtc tgcggctcgg
    13621 ggttgaagtc ggtgatgatg gccgcgcagg gcatccaact cggcgactcg gaggtggtcg
    13681 tggcgggcgg catggagtcg atgtcgaacg cgccgtacct gcttccgaag gcgcgcgagg
    13741 gctaccggat ggggaacgcc cagatcgtcg attcgatgat caacgacggg ttgtggtgcg
    13801 cgttcgagaa ctatcacatg ggcaacaccg gcgaggtcgt cgccgaacgc tacggcgtga
    13861 cgcgcgccga gcaggacgag tatgcctaca actcgcaccg caaggccgcg gcggcgatta
    13921 aggccgggaa gtttaaggac gagatcgtgc ccgtcgagat tccgcagaag aagggcgcgc
    13981 ccgtcctgtt cgacacggac gagacggtgc gcgaagatac gtcggtcgag gttctcggca
    14041 agctccgccc ggccttcaaa aaggaaggcg ggacggtgac ggccggcaac gcgccgggcg
    14101 tcaacgacgg ggcgtcggcg ctggtcgtga cctcgcttga gcgggcgcgg gagatgggcg
    14161 cggagccgct ggcgcgcatc gtggcgcaag ccacgtcggg gatacagccg gagctggtga
    14221 tgatggcgcc cgtcgaggcc atccgcaaag ttgtgagcaa ggccgggtgg tcgctgagcg
    14281 aggttgactt gatcgagttg aacgaagcct tctcggtgca ggccatcgcc atcatgcgcg
    14341 agcttgagct tgacccggcg cgcgtcaacg tcaacggcgg ggcggtcgcc ctcggccacg
    14401 ccatcgggca atccggctcg cgcctgttga cgacgatgct ctacgagatg cgccgcacgg
    14461 gcgcccgtcg cggactcgcg gcgctgtgcc tcggcggcgg caacgccgtc gcgctggccg
    14521 ttgagaggta ggattaaacc agtgataagt gatgagtgat aagtgataag aaaatcggct
    14581 tgctctttct tatcacttat cactcatcac ttatcaccat aaaggttgta acgtgacacc
    14641 ggctgaccac aacaaagcaa tcggcatcat gcatctcatc tacggtgggt tctttacgtt
    14701 gatgacgctg atgatgctcg tcttcttcgg cttcttcgcg gccctgatga gcagcattcc
    14761 gcaggagccg ggcgcgccgc cggccgcgtt cttctcggcc atcttcggct tcatcgtcgt
    14821 catctacgcg ctgctgagca tcccgagtct cgtggccggc tacgcgttgt tgaagcggaa
    14881 atcgtgggcg cgcgtcgcgg ggatagtggc gtcgattctg gcgggcgtca gcttcccgtt
    14941 cgggacggcc ctgtgcgtct actcgctgtg gtacttcttt ggcgagcagg gtcgagacta
    15001 cgaggcccga ctcgcgggcg cgcaccgttg gcccggctca ctcaaccatg ccgccccctc
    15061 cggttggagc gcgcagcagc agcaacagca acaacagcag gacgcggaat cccggcagag
    15121 cgcctaccac ccgccgcctt cgccgccgga ctggcgcggg tgaaagattt ttaatatcgg
    15181 agagcaaaag gtgagcgaga caatcggtgt ggtcggagcg gggacgatgg gcaacggcat
    15241 cgcgcaggtg gcggcgcgcg ccggccaccg cgtgctgctg cgcgacgtgg cccccgaatt
    15301 cctcgaacgc gggatggggg ccattgaccg cagcctccag cgcgacgttg ataaggaacg
    15361 cctgaaggcc gaagagaaac gcgagatcgt cggccgcatc gagacgacga cggagcttgc
    15421 ggcgctcgcc ggcgccgact tcatcgtcga ggccgtgacg gagaagctgg aggtcaaggc
    15481 ggaagtcttc cggcagctcg acgagatagc gaagcccgaa gccatcctcg cctccaacac
    15541 gtcgtccatc tcgatcacca aactgggcgc ggccacgtgc cgggccgacc gcgtcatcgg
    15601 catgcacttc atgaacccgg tgcccgtgat gaaactcgtc gaagtgatcc gcggcatcgc
    15661 cacctcggac gacacgtggg agcggacgcg cgcgctcacc gaacgcctcg gcaagacggc
    15721 gctcgaatgc cacgactcgc ccggcttcgt ctccaaccgc gtgctgatgc cgatgatcaa
    15781 cgaggccatc ttcgcgctcc acgagggcgt cgccacgcgc gagtcaatcg acggcatcat
    15841 gaagctcggc atgaatcatc cgatggggcc gctcacgctc gccgacttca tcggcctcga
    15901 cgtgtgcctc gccatcctca acgtcctgca cacggaactg ggcgacccga agtatagagc
    15961 gtgccctctg ctcaaacgct acgtggacgc cggctggctc ggcaggaagt ccgggcgcgg
    16021 cttctatga
//
LOCUS       DASVES010000393        20375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_842307, whole genome shotgun sequence.
ACCESSION   DASVES010000393 DASVES010000000
VERSION     DASVES010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20375
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842307"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..567)
                     /locus_tag="VF240_16060"
     CDS             complement(325..567)
                     /locus_tag="VF240_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176780.1"
                     /translation="MIRIDYDHQANYRGRDPEFAAEQLAKFGRFGKGAFGSNPVFDAE
                     HLYNFPHLSGSKDGRSNTKVFVPGNFKGTPTGPTPT"
     gene            complement(564..1442)
                     /locus_tag="VF240_16065"
     CDS             complement(564..1442)
                     /locus_tag="VF240_16065"
                     /inference="COORDINATES: protein motif:HMM:NF033520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEX7176781.1"
                     /translation="METKLIALYLLICRLYDTKPVLKQQRLSNNHQPLFSDEELLTIY
                     MFGHLQGHTSGRRIYDYIRHHWRVWFPALPAYQAFNRRLNELAPAFELLIEELLTSAR
                     WQIQPNDDRLIDSMPVMLARGTRANRARVAHGLADTGFCATKQQHYRGVKLHVLAARR
                     SAKLPLPEKIHLSKASQHDLAALRELDPLMPANSGLFADKAYFHAATETEFKERGSFL
                     VASYKRHRNEPETNVPTLSNRFVSTIRQPLESLFNWLIQRMDLQNASRVRSSQGLKVH
                     CYGKLAVACLLLTFYS"
     gene            1505..2239
                     /locus_tag="VF240_16070"
     CDS             1505..2239
                     /locus_tag="VF240_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176782.1"
                     /translation="MHTSEEWVKAAHFSQVQHQRYNPIHESGTGTEMILRFLKYSLIT
                     LLLCLAYDLTNPCVGQAKHNNKRHAPTTALQNPPVGQGINAPKPPVKKKTLLTTLRLK
                     GLSDAELIKLVTEQGVEFYLTGEDEHELRSAGASAALIDAVRKNVKTDIDGAGRIRIL
                     ADSSLSNYFAYRRGHRFYVVMPGKNFSNLESTVSSSPELGAIEMQWLQLDTDTVFSLA
                     LPQGVMARVEQSGEQLDVILVRRISK"
     gene            complement(2436..2624)
                     /locus_tag="VF240_16075"
     CDS             complement(2436..2624)
                     /locus_tag="VF240_16075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7176783.1"
                     /translation="MEFLTTKQVAERLGITPRRVQALVTAGRLPATKFGRDYLIKEKD
                     LKLVEERKVGRPKKTEQK"
     gene            complement(2862..4352)
                     /locus_tag="VF240_16080"
     CDS             complement(2862..4352)
                     /locus_tag="VF240_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7176784.1"
                     /translation="MNVPAILQAAATGHTAVVQSLLAEGVDINEKGRNGDTALMRAAA
                     NGQLETVQLLLDEGADINAQRGDGMTALILAAFFGHLDVVLALINKGANLSARDRTGM
                     TALDRARSKGETDTVNILTSTRTIDAQTSVNERGAGRPITSKVTEELLSQSSRADSDG
                     SVDDILDLPSAPGLLTSDTQRDTPLQLEGDLLSPEPPRDLSQSELTEPPGVSRVPPVE
                     PRTALSQHAGRVVDALRESDPAGEGGLADDNFNVPETVSPISSPEGLQTGLHAHSKRV
                     LKNGIPAATFGPVNETPLQSKPVSSLKYASLFLAIMFGSAALTWTIVLRMGGFPNSYE
                     APSILPSQGEGAANRIGGAGTGTPTKGESPQKGARASSDDAVLTAALGDWVAATNAKD
                     IEKQMSFYAPRLDTFYRLRNVSSSIVRDEKINFFARAREVHISTSEPEIRYSRNALRA
                     SMRFRKVYLIDIGLEVLRGEIIQELVWRKTKNGWKVVGERDVKVIR"
     gene            complement(4679..4752)
                     /locus_tag="VF240_16085"
     tRNA            complement(4679..4752)
                     /locus_tag="VF240_16085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4716..4718),aa:Arg,seq:acg)
     gene            complement(4869..5906)
                     /locus_tag="VF240_16090"
     CDS             complement(4869..5906)
                     /locus_tag="VF240_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7176785.1"
                     /translation="MGTRGFFHKISMRLLCAAALLLAACVFGAAPRARAQDDDTVRVE
                     SNLVLLNVGVADRRGRAVTDLARGDFAVYEDGVRQNITSFEPTNAPFSLVILLDTSGS
                     TLSFRTTLKQAAVRFVDALAPDDRIAVVSFNEKVKTLQSFTTDRKKIGFAILELAEGR
                     GQTSLYKGVRHALAALAEEGNRRKAVVVITDGKDSELERSDRVSAATSGTGAQSVAIL
                     KPESSTALRAALDMADRQGVTVYPLALPSGDLRLVHDPTPQQVAVYTAARARLQELAN
                     RTGGRLHVIQRLEDMGRLYAEVAADMRTLYTITYQPAGERKRDGAWRAINIEVSRAEL
                     IARTRPGYYSR"
     gene            complement(6213..6881)
                     /locus_tag="VF240_16095"
     CDS             complement(6213..6881)
                     /locus_tag="VF240_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176786.1"
                     /translation="MRLRLYCNRYGHFKDALVCSVNCVYRTRCHDFALYYDAHRAEVD
                     AEVTDYYDARREGRGGEAASARGASVAGASADAPARAPKLVTPVEMRALIRLEVKREM
                     SEVVFIWVDKDDRAELVETPEVLRRAERGQKPKHIYKVSQEMELRYQLVPRKSIDRAK
                     RAAAVDEERAVARRRARTQKTPPAPVPFPAPVPAAQHDEASAAASPARRTRARSRVAK
                     IAGR"
     gene            7201..7689
                     /locus_tag="VF240_16100"
     CDS             7201..7689
                     /locus_tag="VF240_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176787.1"
                     /translation="MYKPNFCAECGERVARKRWRVWTSRRFCPACDVRGRTRRITAPL
                     ALGACLVVCGFVLGRAGRPAPPPLVVVRGGAATGEIASRDASGGSPTDEGGAGRPAPG
                     YGADGTASERPTDPLEVVSICGARTKKGTPCSRRVRGSGRCWQHRGKPAVLPPQKLIV
                     PG"
     gene            complement(7990..9012)
                     /locus_tag="VF240_16105"
     CDS             complement(7990..9012)
                     /locus_tag="VF240_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7176788.1"
                     /translation="MKITRLAQTFAFALLLALAPTALAQESTSTQTATTSVASGQKMK
                     LKGVVTRRDADTFTVQADGGGLTTVLLTDRTSVKTKGGFFGGGTNYATTNLLRGLRVE
                     VEGRGDSSGQLVAEKVRFGDKDLRVAQSIEARVDPVESRVGTAEGRIGTVEQNAQRMS
                     GQLDELAAVSNAARGGAVAALETAESAVAGVNATNERISALDDFTAQQSLNVSFKHRS
                     AVLTPDAKLQLDEMVKQTTNARGYMLEVSGYAYDFRAKESNRRLSQQRADAVVRYLAE
                     NHRIPLRRIVTPFGYGNTVEPIADNTTREGRAENRRAEVRLLVNKGLTGSAPMMNEGK
                     VSTATP"
     gene            complement(9283..10671)
                     /locus_tag="VF240_16110"
     CDS             complement(9283..10671)
                     /locus_tag="VF240_16110"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7176789.1"
                     /translation="MTTTHAPRLLRRTRAGRLALIALSAALVVVVLPTLARDARAQDT
                     VGPAPSEMAPPGAFVIRGARIVTVSGPDIENGTLVLRNGRIEAVGTNVNVPAGATEID
                     ARGLLVYPGLIDAETNLGLGEISSGAPGTLDTTELGDMNPNAQAWVAVNPHTSHLEVT
                     RVAGVTSVVSMPQGGVVSGQAAFLNLAGTTQRELAIVPTVALVIDYPRVGGGFGFGGG
                     AAVTPEALTARDRRVDELRRMLRDAEAYGRAADAYRRDPKAVPRPDTDLVLAALVPYV
                     RGERPVMFRADRERDIRAAVGFAEEMKLRPVVVGGSEAWKVATFLKEKNVPVIFDAMW
                     GLPLREDDPYDTLYESPAKLQQAGVRFAISTGDGGAHVRDLPFQAGMAVAFGLPKDEA
                     LKSITLYPAQMMGVADRVGSIEAGKLANIVVTDGDILDARTNVRHLFIGGRQIPLVSR
                     HTVLYEQSKDRP"
     gene            complement(10668..12170)
                     /locus_tag="VF240_16115"
     CDS             complement(10668..12170)
                     /locus_tag="VF240_16115"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7176790.1"
                     /translation="MMRRLTALAICLALTLSWWGASGARAQQTQRSGGAQRSGGAATT
                     TTAAAQGGETLIKGATVLTVTKGRLENTDVLIRNGKIAGVGPGLRAGASARVIDAAGK
                     FVMPGIIDAHSHAMIDGSVNECTLSVTSMTRVQDVLNPTDVNLYRHLAGGVTSLLLLH
                     GSCNAIGGQSATVKIKYGRPLAEFPFPGAPPGIKFALGENPKRTNQPTAPGQQRRYPA
                     TRMGVEEVIRQAFTRARDYRAAWDEYRAASRTNKSLFPPRRDLQLEPLVEILEGKRYV
                     HAHSYRADEILMLIGIADEFGFKIRTFQHVLEGYKVAGEIARHGAGASSFADFWSYKM
                     EAYDAIPYNAAIMTRHGVNVSINSDSSERARRLNVDAAKMMKYGGLTEEEALKLITWN
                     PAWQLGIQERVGSIEVGKDADLAIWTGHPLSTMSRVETTFVDGDIFFDRNLDFARRNA
                     LEGERKALEGAEPNRAPTQGGTPPTAPRGRPSRASDAHDDDQIIDGGDDQ"
     gene            complement(12267..14351)
                     /locus_tag="VF240_16120"
     CDS             complement(12267..14351)
                     /locus_tag="VF240_16120"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7176791.1"
                     /translation="MRNRPQRIFALFAAAGVALSALQAVPGSAQQTLPAATRGVGTDT
                     YAITNARVVTVSGPVIERGTVVVRDGLIAAVGERVAVPPDARNIDGAGLTVYPGVIDA
                     STNLAIPRPTPAASPGGGGGGFPGFQPVQVSATSPNSSQLPGLQPEILAADIIRPGGA
                     EVEASRNAGITAALTTPRGAVFLGQSALINLSGDTPQQMIVRSPVALHVGLSPIGQGQ
                     YPGSLMGVLTTIRQMLLDAQRYRDSNEIYDRNPRGLRRPGPDKSLAALLPVLARQVPV
                     VMQAEREREITRALDIAQEFNLRLIINGGAEADRLAARLKAQNVPVLLTLNFPLRSAT
                     PSPEADPEPTRVLRERVEAPKVAGRLAQAGVRFAFQSGGMTSMADYLANAAKAVDAGL
                     ARDEAVRALTLRPAEILGVADRLGTIEAGKIANLTVTRGDIFAKDRRIAHVFIDGRPV
                     DLRPSATAAPGATNATGNWSLRVNLGGGQQEVSVTLNLQQQGERLTGSIQGPLGSGQI
                     GAGSLAGADLRFTVPVTLPGPGSQTTDATFTGTLTGNEMRGTVQVVGRDPGTFTGART
                     GGPTPSPGGAAGTGTQPAPAAAPTPDAAAVPDLSGNWSIEVDVGERVIAATLQFQQSG
                     ATLSGSMQSEFGTSPISGGSVRPDGFRFSTQVNIGQVVDVTFEGTASGNTMQGTANAP
                     TGAASFTGRRTP"
     gene            complement(14669..15181)
                     /locus_tag="VF240_16125"
     CDS             complement(14669..15181)
                     /locus_tag="VF240_16125"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7176792.1"
                     /translation="MNQRTLAALLAVAALAITAACGGDSNTNANRVNTNTANRAATPT
                     ATPTPANTNRAVNANITRDEYNREQARYDDDARRSGRTIGSGINDRWLWTKVRAQLLG
                     ADDLRESTINVDVENDVVTLTGSVANGNQKIRAADIAKKTDGVKSVKNNLTVNASGSN
                     SNAAPAANRK"
     gene            complement(15606..15908)
                     /locus_tag="VF240_16130"
     CDS             complement(15606..15908)
                     /locus_tag="VF240_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176793.1"
                     /translation="MKKSKIAALVAGTALAALTFACGGDANHNGNANVSNLNTNRPAN
                     TSISNANVTISNSNVTVVPPANTSSNTSSMNGNMSGSNMNMSRSNTNAGATNANRP"
     gene            complement(16199..16483)
                     /locus_tag="VF240_16135"
     CDS             complement(16199..16483)
                     /locus_tag="VF240_16135"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX7176794.1"
                     /translation="MADEIKCARCGQKRDALGYAPYPTELGQKIGAEVCQQCWSEWLQ
                     KQQMIINHYGLNLMEPDAQAFLFDNAKGFFFTAGQDLAQIDTTKEGTVKW"
     gene            complement(16590..17087)
                     /locus_tag="VF240_16140"
     CDS             complement(16590..17087)
                     /locus_tag="VF240_16140"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7176795.1"
                     /translation="MNEQTGPQVGQKAPDFTLPDGDGNDWRLSEQRGRVVVLLFYPGD
                     ETPVCTRQLCAVRDRWEDYSGTGAEVVGISTDSVEKHRSFAENRRLPLRLLSDPEARV
                     VKLYGVASWLPGRAARAVVVIDPRGRVSYRRVQPLSLLRPKDDDVLAAIRAASSDGDD
                     AAEGP"
     gene            complement(17084..18106)
                     /locus_tag="VF240_16145"
     CDS             complement(17084..18106)
                     /locus_tag="VF240_16145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7176796.1"
                     /translation="MNEQRPSYLALYESGELRRRVEALELLLARCTVCPLDCGNNRTK
                     GELARCYSGRLPVVSSYTPHFGEEPALVGTGGAGNVFFGNCNLRCVYCQNYQISQTHR
                     EQIKNEVTHERLAGMLLELQARGCHNINFVSPTHFAPQMARAILIACSGGLRLPVVYN
                     TNAYDSVEVLRLLDGVVDIYLPDLKYADSSEGYAFSKVPGYAEHARAALAEMYRQTGD
                     RLVFDDAGLLQRGLVVRLLVLPNDVAGVRDSLEWIRETLSPRVAVSLMAQYYATNKAA
                     HDERFILLSRRITEREWLRAVSALEELGMEEGWLQEYDGAAHYYRPDFNNPETPFKDI
                     RDFQKT"
     gene            complement(18103..18657)
                     /locus_tag="VF240_16150"
     CDS             complement(18103..18657)
                     /locus_tag="VF240_16150"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX7176797.1"
                     /translation="MDALSSALAVLSAMITPAVLISACGTFILSTSVRLGRVVDRVRS
                     LSDRFEEMARTEGEVQLFEERRAVIFNQLDMLTSRARILQRSMTVFYTAGGIFVLTSV
                     AIGVVAAVDGARYSWVPVAMGLLGSLFLFWGSVLLILEARLALSTITAEMDFIWQLGR
                     TLAPKHLVDEHSPHHNRLTLRRRP"
     gene            18946..20313
                     /locus_tag="VF240_16155"
     CDS             18946..20313
                     /locus_tag="VF240_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176798.1"
                     /translation="MSDKDLEDRDDATGGGGGESGGLSDGDEGSGGGGDAPETGARGG
                     GLAIGRATGVGGGGAEGGDAGGGTAVGEAGGGSSTGGGSGSTGGGGATGGGSSSSTSG
                     GGSSSTSGGGSGGASSGGTSGGGETTGSGGGGGRGGSRGASGGGSTGGAGGGGASGGG
                     RGASGGSSSGGSGGSAGGGGSARSGGTSGGGGALGSGGGGGGLRSGSGAAKSGAAGGA
                     RSGSAGVGTKGGSSKGGSRAGSAAARGGSSAGATGGGGGARGSGGGATKGAGVVTGGG
                     KAGAARAGAKAGSTKGGAKKGGATKSSAATRGGVAGGRGGAGGGAARGGGVGASKGGA
                     RSGTAAGRGGAGKGAAKKGGASKSGATRGGTAGRGGSGAASRGGAATGGAGGRGGAGA
                     GRSGVAKGGARGGAGGASKSGAARKGGGGGRPAGGGTARGTGGGRGGSKGGGSKSGGS
                     RRGGG"
BASE COUNT         3560 a         5980 c         7041 g         3794 t
ORIGIN      
        1 tccctgacgg cgtcccataa gccatatccg agtatcggca gtaaatatca aacggtgatg
       61 atctgacttc ttcgttccgg tgcgcccggc gaggctcgcc acgacggccg ccaactccat
      121 cggctcgacg ggcttcgcca catgcacctg gaagccggtg aggagcgccc gcgtgtggca
      181 gttttacagg tagtgttcta tgaaagattg gcacccactc ggacccaagt ggcggcggtc
      241 tcgacgggtc taccctcgcc cccccgctgc cgagcaaccg catcgtcatc gcgcagtcga
      301 agaccacgcc gtcgagcgta cagctcaggt cggagtaggt cctgtgggcg tccccttaaa
      361 gtttccagga acgaacacct tggtgttact tctgccgtct ttgctacctg aaagatgcgg
      421 gaaattatag aggtgctctg catcaaaaac tggattacta ccgaatgcac ctttaccgaa
      481 acggccgaat ttagcgagct gttcagcggc aaattctgga tcgcgtccac ggtaattcgc
      541 ctgatgatca taatctatgc gaatcaagag tagaaagtga gcaacaaaca ggcgacggcg
      601 agtttgccat agcaatgcac tttcagccct tgacttgagc gaacgcgcga agcgttctgt
      661 agatccattc tttgaatcag ccagttgaat aaactttcca gcggctggcg aatcgttgag
      721 acaaagcgat tggagagcgt cggcacgttg gtttcaggtt cgttgcgatg acgtttgtat
      781 gatgccacca gaaatgaccc gcgctctttg aactccgttt ccgtggcggc gtgaaaataa
      841 gccttgtcgg cgaacaatcc gctgtttgcg ggcatcagcg ggtcaagttc gcgcaaggct
      901 gctaaatcat gttgcgaggc ttttgataag tgaatcttct ccggcaacgg caacttcgcg
      961 cttcgccgcg cggcaagcac atgcagcttg acgcccctgt aatgctgttg tttggtggcg
     1021 caaaagcctg tatccgctaa tccatgagcg actcttgcgc gattggcgcg tgtgccgcgc
     1081 gccagcatca cgggcatcga gtcaatgagt cggtcatcat tgggttgaat ttgccatcgg
     1141 gcactggtta agagttcttc aatgaggagt tcaaaggccg gggcaagctc attcaagcgg
     1201 cggttgaagg cttgataggc aggcaaggca ggaaaccaca ctcgccaatg gtgacggatg
     1261 taatcataga tgcgccgccc gctcgtgtga ccttggagat gtccaaacat gtagatcgtc
     1321 aaaagctcct cgtccgagaa tagcggctga tgattattgc tcaagcgttg ctgtttgagg
     1381 acgggtttcg tatcgtagag gcggcagatg agcaggtaga gtgcgataag tttagtttcc
     1441 acaaaaccat cttatcgcct cttgctcatc tgctttttct attcttgatc cgcataagct
     1501 atacatgcat acctcagaag aatgggtcaa ggcggctcac ttttctcaag ttcaacatca
     1561 gagatataat cccattcatg aaagcggcac gggtacagaa atgattcttc gttttcttaa
     1621 atattcatta ataacgctac tgctttgtct tgcatatgac ctcactaacc cgtgtgtcgg
     1681 tcaagctaaa cataataaca agagacatgc acccaccacc gcattacaaa atccgcctgt
     1741 aggccaagga ataaatgccc ccaaaccccc ggtaaagaaa aagaccctcc tgactacatt
     1801 gcgcctaaaa gggctgtctg acgcagagtt gattaaactt gtcacagagc agggcgttga
     1861 attctactta acaggtgaag atgagcacga gttgcgctcg gcgggcgctt cagcagcatt
     1921 gattgacgct gtacgtaaga atgtcaaaac ggatattgac ggtgcgggaa gaataaggat
     1981 tctcgctgac tcctcactta gcaactactt cgcttatcgt agaggccacc gattttatgt
     2041 cgtgatgccg ggaaagaact tttctaattt agaaagcacg gtttcgtcat cgcccgaatt
     2101 aggggctata gaaatgcaat ggctacaact ggacacggat acggtgttct cgttggcact
     2161 gccgcaggga gtaatggcgc gcgtcgaaca gagcggtgag caactagatg taatcttagt
     2221 gagacgtatt tctaaataga gagtaatagc cagccacttc taacggcaat agttacaaaa
     2281 tattagtaga ctaaaactag atgtacatca tcttgaaatg gctaattgca aataacaaaa
     2341 aaataaaaaa tatttctgct cgaatcaaat tgctagttca gcaaaaacag cacccctacc
     2401 cttagacaac ttgaggcaag tccaaatcta aaattctatt tctgctcagt cttcttcggc
     2461 cttccaactt ttctttcttc tactaacttc aaatccttct ctttaatcag ataatctcga
     2521 ccgaatttag ttgctggcaa tcttccagcc gtgacaagtg cctgaactct tctaggcgtg
     2581 attcctaatc tttctgctac ttgtttggta gttaaaaatt ccataggaaa atagttatag
     2641 aactaaagta attattcgtc aagaagaaga aaaagattga tattttatac ttttaggagt
     2701 ataaataatc aaaaggggaa cataaggggg aaataagaaa aggccactat ttccagtagc
     2761 cttaacttat tgatttattt aatgcaccct gcatgactcg aacgtgcgac cttccgattt
     2821 caaagctcta cggaagagtg cccacagcgt aaggcggttt atcaccttat gaccttaaca
     2881 tcacgttcgc caacgacctt ccacccgttt ttcgtcttcc gccagaccaa ttcctgaatg
     2941 atctctccac gtaacacctc cagcccgatg tcaatcaaat aaactttgcg aaaacgcatg
     3001 gaggctctga gggcgtttcg gctgtatctg atctcgggct cgctcgtgct gatgtgaacc
     3061 tcccgtgccc gcgcaaagaa gttgattttt tcatcgcgga cgatgctgct cgacacattt
     3121 cgcagacggt agaaggtatc gagcctgggc gcgtaaaaac tcatctgctt ttctatgtct
     3181 ttagcattgg tcgccgctac ccagtcaccc aacgccgcgg tcaaaactgc atcgtcgctg
     3241 cttgctcggg cgcccttctg cggcgactcc ccctttgtcg gagtgccagt cccggctccg
     3301 ccaatccgat tagctgcacc ttcgccctga gacgggagaa ttgatggcgc ctcgtagctg
     3361 tttgggaagc ctcccatccg taggacaata gtccaggtta aagcagcaga gccaaacatg
     3421 atcgcgagaa acagactggc gtacttaagc gaggaaacag gtttgctttg aagaggggtc
     3481 tcattaacgg ggccgaatgt cgccgcagga attccatttt tgaggactcg cttggagtga
     3541 gcgtgcagtc cggtttgtaa accctccggg gatgatatcg gtgaaaccgt ttcggggaca
     3601 ttaaagttat cgtccgcaag gccgccctcc cccgcgggat cggattcgcg cagcgcgtcc
     3661 acaaccctgc cagcatgttg agacaacgcc gtgcgtggtt ccacaggagg aacgcgcgag
     3721 acgccaggcg gttcagtcaa ctcggactgt gataaatccc tcggcggttc aggcgataac
     3781 agatctccct ctaattgaag cggggtgtca cgttgagtat cagatgtcag aagccctggc
     3841 gcgctgggca ggtcgagtat atcgtcaacc gacccatcag aatctgccct cgaactctgt
     3901 gatagcaatt cttcagttac ttttgatgtg attggccgtc ctgcccctcg ctcattcact
     3961 gacgtttggg cgtcaattgt tctggtagaa gttagaatat ttacagtgtc cgtctcgccc
     4021 tttgacctcg cccgatccag ggccgtcatg ccggtcctgt ctctggcgct taaattggcg
     4081 cctttgttaa tcagggccag cacaacgtcc aaatggccga aaaaggcagc gaggattaag
     4141 gctgtcatcc catcgcccct ttgtgcattt atgtcggccc cctcgtccag caaaagctgt
     4201 acggtttcta attgcccatt cgccgcagcg cgcatcaggg cggtatctcc gttacggcct
     4261 ttttcattga tgtccacgcc ctcagccaat aaagactgaa ccacagccgt atgcccggtc
     4321 gctgccgcct gtaatattgc cggcacattc ataaatccga actcacttaa cccgcgattt
     4381 tgattcaccc gtgcggaata ataacgctac tggcggtcta acaggactgt agatgataat
     4441 gaccgctgac tacagcaaga ctcaaccttt aatttgacga atactgtaat tccacagaaa
     4501 ggttcttctg ttcaaatttt actcacactc ttcacgacaa cccgtgccag cccttacacg
     4561 caagcaagca cagtcaatac atgcaaagat atctgtgcct tggaagttgg cacactatag
     4621 gcacacaaaa caagaaaggg cagccgcgcg gctaccctta acttattgac ctgattaatg
     4681 cacccggcac gactcgaacg tgcgaccttc tgattcgtag tcagacgctc tatccaactg
     4741 agctacgggt gcgtggcggt ggaagtggga aaaaatatac gggcggtcaa ggtgtgtgtc
     4801 aagcgagcgt aaggctgagg aagttgggcc ggttgagact cacgcgaacg ggcgtttgag
     4861 ggtgggggtc agcgcgagta gtagccgggg cgggtgcggg cgatgagttc ggcgcgggag
     4921 acttcgatgt tgatggcgcg ccaggcgccg tcgcgcttgc gctcgccggc gggctggtag
     4981 gtgatggtat agagggtgcg catgtcggcg gcgacctcgg cgtagaggcg gcccatgtct
     5041 tcgaggcgtt ggatcacgtg caggcgtccg cccgtgcggt tggccaactc ctggagacgt
     5101 gcacgggcgg ccgtgtagac ggccacctgc tgcggcgtcg ggtcgtggac gagtcgcagg
     5161 tcgcccgagg gcagcgcgag cgggtagacg gtgacgccct gccggtcggc catgtcgagc
     5221 gccgcgcgca gggccgtgct cgactccggc ttgaggatgg cgaccgactg tgcgcccgtg
     5281 cccgacgtgg cggcggagac gcggtcggag cgctccagct cggagtcctt gccgtccgtg
     5341 atgacgacga cggccttgcg ccggttgccc tcttcggcga gcgcggcgag cgcgtgcctg
     5401 acgcccttgt agagagaggt ctggccgcgt ccttcggcca gctcgaggat ggcgaagccg
     5461 atcttcttgc ggtcggtcgt aaacgactga agggtcttga ccttctcgtt gaaggagacg
     5521 acggcgatgc ggtcgtcggg cgcaagcgca tcgacgaagc ggacggccgc ctgcttcaag
     5581 gtggtgcgga agctgagggt ggagccggag gtgtcgagga ggatgacgag actgaagggc
     5641 gcgttcgtgg gctcgaagct ggtgatgttc tgccggacgc cgtcctcgta gacggcgaag
     5701 tcgccgcgcg cgaggtcggt gacggcgcgc ccgcggcggt cggcgacgcc gacgttgagc
     5761 agcacgaggt tcgactccac gcgcaccgtg tcgtcgtcct gcgcgcgcgc gcgcggcgcg
     5821 gcgccgaaaa cgcaggcggc cagcagcagg gccgccgcgc agagtaatct catcgagatt
     5881 ttgtggaaga agcctcgtgt tcccatcgcg agaaaatgtc tgatcgaaaa ctcagtatgc
     5941 aagggcgcgc gcgccgaacg caagcggcag acgacaccca acacgcgacg cggccgacgc
     6001 gcttaaaagg cgccccgggc cgaaatagcg gcgcagtgtc ggccccttac aggcgtctaa
     6061 ccggccagag agtgtttggt gctcgggagc gggcgggagt tgcacggaag acagggcgga
     6121 gggcggaagg atgaagggtg gaaggattga gtggacgcct cagttaacgc ccttactcga
     6181 cctcttcatc cctccgccct ccgcccttca tttcacctgc cagcgatctt ggcgacgcgg
     6241 gagcgcgcgc gggtgcgtcg tgcgggtgac gcggcggccg acgcctcgtc gtgctgcgcc
     6301 gccgggacgg gcgcggggaa ggggacgggc gcgggcggcg tcttttgcgt gcgcgcgcgg
     6361 cgacgggcca cggcgcgctc ctcgtctacg gccgccgcgc gcttggcgcg gtcgatgctc
     6421 ttgcgcggca cgagctggta gcgcagctcc atctcctgcg agaccttgta gatgtgcttc
     6481 ggcttctgcc cgcgctcggc gcggcgcagc acctcgggcg tttcgaccag ctcggcgcgg
     6541 tcgtccttgt caacccagat aaaaaccact tcggacattt ctctcttaac ctccagacga
     6601 attaacgcgc gcatctcgac cggcgtgacg agcttcggcg cgcgcgccgg cgcgtcggcc
     6661 gacgcgccgg ccactgacgc gccgcgcgcg gacgcggctt cgccgcctcg accttcgcgc
     6721 cgcgcgtcgt agtagtcagt cacctcggcg tccacctcgg cgcgatgcgc gtcgtagtag
     6781 agcgcgaagt cgtgacagcg cgtgcggtag acgcagttga ccgaacagac cagagcgtct
     6841 ttaaagtgcc cgtagcggtt gcagtagagt ctgagacgca tcgttgaaac acgcgccgcg
     6901 cggcggcggt tgcctgcccg cgcgtcttcc ggcgctggcc gaagccgccg ggcgagcggg
     6961 tccgttcggg agcaggagta atttaacacg gacgtgccgc gcgtgcaaca aaagattgtc
     7021 cgtgaaacaa ggggcggata tacgcgtcgg ccagttgggt ttataatccg gcgcgcacgg
     7081 gcgagaaact cacacgccga cttcccacgg aaaaatcgag cgcgcccgcg ccgcccgctt
     7141 agaaagcgtc tacttaataa cggcccgtcg aacctcactt cgaaggaggc gcctcaccga
     7201 atgtacaaac ccaatttctg tgccgagtgc ggcgagcgtg tcgcgcgcaa gcgctggcgc
     7261 gtgtggacga gccggcgctt ctgtcccgcg tgcgacgtgc gcgggcgcac tcggcgaata
     7321 actgcgccgc tcgccctcgg agcctgtctg gtcgtctgtg gattcgtgct cgggcgcgcc
     7381 gggcgacccg ccccgccgcc cctcgtcgtg gtgcgcggag gggcggcgac aggagagatc
     7441 gcgtcgcgag acgcgagtgg aggtagtcca acggatgaag gcggggctgg ccggccggcg
     7501 ccgggttacg gggccgacgg gacggcgtcg gagcgcccga ccgacccgct ggaagtggtc
     7561 tccatctgcg gggcgcgcac gaagaagggg acgccctgct cgcggcgcgt gcgcggcagc
     7621 gggcgctgct ggcagcaccg cggcaaaccg gccgtgctgc cgccgcaaaa attaatcgtg
     7681 ccgggttgaa ggttggatat tgaacggagg acggggactg acgctcttcg gagcggtctc
     7741 aagggccggg agtcggcgag ggagtgcgga ggggcgagcc tgacgctggg gtcgtcgcca
     7801 aagaaaaaaa ttggcggcag gttcccccat aaacctgccg ccgcggtcgg ccacttgcgt
     7861 ggcctgcctt aaagtttcac tcagtcccag aaggattgtc cctgaaacga atcatacaga
     7921 cgcgaccccc gacgtaggtt gcggttatgt tgcgtctgcg tgacgttacg cttgccgagt
     7981 cccgttcggt tacggcgtgg ccgtcgagac cttgccctcg ttcatcatgg gggccgaccc
     8041 cgtcaggccc ttgttgacga gcaggcgaac ttcggcgcgg cggttctcgg cgcggccttc
     8101 gcgcgtcgtg ttgtcggcga tgggttcgac cgtgttgccg tagccgaacg gggtgacgat
     8161 gcgccggagc gggatgcggt ggttctcggc cagatagcgc acgacggcgt cggcgcgctg
     8221 ctggctcagg cggcgattcg actccttggc ccggaagtcg taggcgtagc cggagacttc
     8281 gagcatgtag ccccgggcgt tggtcgtttg cttgaccatc tcgtcgagct ggagtttggc
     8341 gtcgggcgtg agcacggcgc tgcggtgctt gaagctgacg ttgaggctct gctgggcggt
     8401 gaagtcgtcg agggccgaga tgcgctcgtt ggtggcgttg acgccggcga cggccgactc
     8461 ggcggtctcc agcgcggcca cggcgccgcc ccgcgcggcg ttcgacacgg cggccagctc
     8521 atctaattga cccgacatcc gctgcgcgtt ctgctcaacg gtgccgatgc gcccctcggc
     8581 cgtgccgacg cgcgactcca ccggatcgac gcgcgcctca atcgactgcg cgacccgcaa
     8641 gtccttgtcg ccgaacctga ccttctcggc cacgagctgc cccgaggagt cgccgcgccc
     8701 ctcgacctcg acacggagtc cgcgcaggag gttcgtcgtc gcgtagttgg ttccgccgcc
     8761 gaagaagccg cccttcgtct tgacgcttgt gcggtcggtc agaaggactg tcgtcagccc
     8821 gccgccgtcg gcctgaacgg tgaacgtgtc ggcgtcgcgg cgagtcacca cgcccttgag
     8881 cttcatcttc tgcccgctcg ccaccgaggt ggtcgcggtc tgcgtggaag tgctctcctg
     8941 cgccagcgcc gtgggcgcga gcgcgagcag cagggcgaaa gcgaaggttt gcgccagtcg
     9001 ggtaattttc atgtgttgtg tcctctccga agttttaagt ttcgtgtcca aagtgtgtag
     9061 ccagcggaaa tgaaggcgac gggcgaggtc gcaacgggca cctgattctt ctggctgtat
     9121 tcagtattcc agacaagtta cccgatttgg gtcgccccgt tggttgcgat tgctttgtga
     9181 atctcgtgta agcaggttgc gaaaaaaggc aaaaggcaaa agtgaagagg cggccgagcc
     9241 ttcccacttt tgccttttgc ctttttactt ttgccttgag tcctacggtc tgtccttgga
     9301 ctgttcgtaa agcactgtgt ggcggctgac gaggggaatc tgccggccgc cgatgaagag
     9361 gtgtcggacg ttggtgcgcg cgtcgaggat gtcgccgtcg gtgacgacga tgttggcgag
     9421 ttttcccgcc tcgatggagc cgacgcggtc ggcgacgccc atcatctgcg ccgggtagag
     9481 cgtgatcgac ttcagcgcct cgtccttcgg cagaccgaac gcgacggcca tgccggcctg
     9541 gaaggggagg tcgcggacgt gcgcgccgcc gtcgcccgtg gagatggcga agcgcacgcc
     9601 ggcctgctga agcttggccg ggctttcgta gagcgtgtcg taggggtcgt cctcgcgcag
     9661 ggggagtccc cacatggcgt cgaagatgac ggggacgttc ttttccttga ggaacgtcgc
     9721 caccttccac gcctcgctcc cgccgacgac gacggggcgg agcttcatct cctcggcgaa
     9781 gccgacggcc gcgcggatgt cgcgctcgcg gtcggcgcga aacattacgg gccgctcgcc
     9841 gcgcacgtag ggcacgaggg ccgcgagcac gaggtcggtg tcgggacgcg gcacagcttt
     9901 ggggtcgcgc ctgtaagcgt ctgccgcgcg gccgtaggct tcggcgtcgc ggagcatccg
     9961 gcgcagctcg tcaacgcggc ggtcgcgggc cgtgagggct tcgggcgtga cggccgcgcc
    10021 cccgccgaag ccgaagccgc cgccgacgcg cgggtagtcg atgacgagcg cgaccgtggg
    10081 gacaatggcc agctcgcgtt gcgtcgtgcc ggcgaggttg aggaaggcgg cctgaccgga
    10141 gaccacgccg ccctgcggca tggagacgac gctcgtgacg ccggccacgc gcgtcacttc
    10201 gaggtgcgac gtgtgcgggt tgacggcgac ccacgcctgc gcgttcgggt tcatgtcgcc
    10261 gagttcggtg gtgtcgaggg tgccgggcgc gccggaggag atttcgccga ggccgaggtt
    10321 ggtctcggcg tcgatgagac cgggatagac gagcagaccc cgcgcgtcga tctcggtggc
    10381 gccggcgggc acgttgacgt tagtgccgac ggcctcgatg cgaccgttgc gaagaacgag
    10441 cgtgccgttc tcgatgtcgg ggccggagac ggtgacgatg cgcgcgcccc ggatgacgaa
    10501 ggcgcccgga ggggccatct ccgagggcgc ggggccgacg gtgtcctgcg cgcgcgcgtc
    10561 gcgggcgagc gtgggcagga cgacgacgac gagggccgcc gacagcgcga tgagtgcgag
    10621 tcggccggcg cgagttcggc gaaggagtcg cggcgcgtgt gtggtcgtca ctggtcgtca
    10681 cctccgtcga tgatttggtc gtcgtcgtgc gcgtcagagg cgcggctggg gcgtccgcgc
    10741 ggggctgtgg gcggcgtgcc gccctgcgtg ggcgcgcggt tcggctcggc cccttcgagg
    10801 gccttgcgct cgccctcaag ggcgttgcgg cgggcgaagt ccagattgcg gtcgaagaag
    10861 atgtcgccgt caacgaaggt cgtctcgact ctcgacatgg tgctcaaggg atggcccgtc
    10921 cagatggcga ggtcggcgtc cttgcccacc tcgattgagc cgacgcgctc ctgtatgccg
    10981 agctgccacg ccgggttcca ggtgatgagc ttgagggctt cctcttcggt gaggccgccg
    11041 tacttcatca tcttggcggc gtccacgttc aaacgccggg cgcgctcgct ggagtcggag
    11101 ttgatcgaga cgttgacgcc gtggcgcgtc atgattgcgg cgttgtaggg gatggcgtcg
    11161 taggcttcca tcttgtacga ccagaagtcg gcgaaggagg aagcgccggc gccgtggcgg
    11221 gcgatctcgc cggcgacttt gtagccttcg agcacgtgct ggaatgtcct gatcttgaag
    11281 ccgaactcgt cggcgatacc gatgagcatc agaatctcgt cggcgcggta gctgtgggcg
    11341 tggacgtacc ttttaccttc gaggatttcg acgagcggtt cgagctggag gtcgcggcgc
    11401 ggcggaaaga gactcttgtt cgtgcgcgac gcggcgcggt actcgtccca cgcggcgcgg
    11461 tagtcgcgcg cgcgcgtgaa cgcctgacgg atcacttcct cgacacccat gcgcgtcgcc
    11521 gggtagcgac gctgctggcc cggcgcggtc ggctggttgg tgcgcttcgg gttttcaccg
    11581 agcgcgaact tgatgcccgg cggcgcgccg gggaagggga actcggcgag cgggcggccg
    11641 tacttgatct tcaccgtggc gctttggccg ccgatggcgt tgcaggagcc gtggaggagg
    11701 aggagcgagg tcacgccgcc ggcgaggtgc cggtagaggt taacgtccgt cgggttgaga
    11761 acgtcctgca cgcgcgtcat cgaggtgacg gagagcgtgc actcgttgac cgagccgtcg
    11821 atcatcgcgt gcgagtgggc gtcgatgatg ccgggcatga cgaacttgcc ggcggcgtcg
    11881 atgacacgcg cgctcgcgcc ggcgcgcagg cccggcccga cgccggcgat cttcccgttg
    11941 cggatcagaa cgtcggtgtt ctccagtcgg cccttcgtca ccgtcaggac ggtggcgccc
    12001 ttgatgagcg tctcgccgcc ctgcgcggcg gccgtcgtgg tggtcgccgc gccgccgcta
    12061 cgctgagcgc cgccgcttct ttgcgtctgc tgcgcgcgcg cgccactagc gccccaccaa
    12121 ctgagcgtca gagccaaaca aatcgcgagt gctgtcagtc gtctcatcat tttctccaag
    12181 ccgtgataag tgatgagtga taagtgataa gaaagagaag gacaattctt gtcttatcac
    12241 ttatcactta tcactcatca ctactgttac ggcgtgcgcc ggcccgtgaa tgaggcggcg
    12301 ccggtcggcg cgttggcggt gccctgcatg gtgttgccgc tcgcggtgcc ctcgaaggtc
    12361 acgtcaacga cctgaccgat gttgacctga gtcgagaagc ggaagccgtc ggggcggacg
    12421 gagccgccgc tgatgggcga cgtgccgaac tcgctctgca tcgagccgga aagcgtcgcg
    12481 cccgactgct ggaactggag cgtcgcggcg atgacgcgct cccccacgtc cacttcaatg
    12541 ctccagttgc cggagaggtc gggcacggcg gcggcatcgg gcgtgggagc ggcagcaggc
    12601 gcgggttgcg tgcccgtccc tgccgcgccg cccggcgaag gtgttggccc gcccgtgcgc
    12661 gcgccggtga acgtgccggg gtcgcggccg acgacctgaa ccgtgccgcg catctcgttg
    12721 cccgtgagcg tgccggtgaa agtggcgtcc gtggtctggc tgcccggccc cggcagcgtg
    12781 acgggaacgg tgaagcggag gtctgcgccc gcgagtgagc cggccccgat ctggcccgag
    12841 ccgagcggcc cctgaatcga ccccgtgaga cgctcgccct gctgctggag gttgagcgtc
    12901 acggaaactt cctgctgacc gccgccgagg ttgacgcgga gcgaccagtt gcccgtggcg
    12961 ttcgtcgcgc cgggcgcggc cgtcgccgac gggcggagat cgacggggcg gccgtcgatg
    13021 aagacgtgcg cgatgcgccg atctttcgcg aagatgtcgc cgcgtgtgac ggtgaggttg
    13081 gcgatcttgc cggcctcgat tgtgccgagg cggtcggcga caccgagtat ctcggccggt
    13141 cggagggtga gcgcgcgcac ggcctcgtcg cgcgcgagtc ccgcatcgac ggccttcgcc
    13201 gcgttggcga ggtagtcggc catcgaggtc atgccgcccg actggaaggc gaagcgcacg
    13261 ccggcctgcg cgagtctgcc ggccaccttc ggcgcttcga cgcgctcacg cagcacgcgc
    13321 gtcggctcgg ggtcggcctc gggcgagggc gtggccgagc ggagcgggaa gttgagcgtc
    13381 aaaaggacgg ggacgttctg cgccttgagt cgcgcggcca gacggtcggc ctccgcgccg
    13441 ccgttgatga tgaggcggag gttgaactcc tgtgcgatgt ctaaggcgcg tgtaatctcg
    13501 cgctcacgct ccgcctgcat caccacgggc acctgccgcg cgagcacggg cagcagtgcg
    13561 gccagcgact tgtccgggcc gggccggcgc aggccgcgcg ggttccggtc gtaaatctcg
    13621 ttcgagtcgc ggtagcgttg tgcgtcgagg agcatctgcc ggatggtcgt caaaacgccc
    13681 atgagcgagc cggggtactg gccctgaccg atgggcgaca gaccgacgtg gagggcgacg
    13741 ggcgagcgca cgatcatctg ctgcggggtg tcgcccgaga gattgatgag cgccgactgc
    13801 ccgaggaaga cagcgccgcg cggcgtggtc agcgccgccg tgatgccggc gttgcgcgaa
    13861 gcctccacct cggcgccgcc gggccggatg atgtcggccg cgaggatttc gggttggagg
    13921 ccgggcagtt ggctggagtt gggcgacgtg gccgagacct gcacgggctg aaagccgggg
    13981 aagcctccgc ccccgccgcc gggcgacgcc gcgggcgtcg ggcgcgggat ggcgagattg
    14041 gtgctcgcgt cgatgacgcc gggatagacg gtcaggcccg cgccgtctat gttgcgcgcg
    14101 tcgggcggca cggcgacgcg ctcgccgacg gccgcgatca acccgtcgcg gacgacgacc
    14161 gtgccgcgct cgatgacggg gccggagacg gtgacgacgc gcgcgttcgt aatggcgtag
    14221 gtgtctgtgc cgacgccccg cgtggcggcg ggcaacgtct gctgcgccga gccggggacg
    14281 gcctgaagcg cactgagcgc gacgcccgcg gcggcgaaga gcgcgaagat tctctgtgga
    14341 cggtttcgca taggagttcg actcccgacc gggaagttag atggaggagc gtcgggcgca
    14401 gcggcgcacg cgccgccgcg cacagaagac gcgccggatt gttttcggta aagctttatt
    14461 ggctgggatg cctgctgcgc ccgcgtgaag cggaagcgcc gccccgcgcc ggaagagacg
    14521 tggggacggg ggccgcttgt tttaacgcgc gccgtcgccg agcaggtgcg cggcgacggc
    14581 gcgaaaagtt ttcgccatgt atcccgccgg cgcacaagcg cacgccgctc atcttcaggg
    14641 cgtgcgcttg cgcgtcagtg gggtaatgtc acttccggtt cgcggcgggc gcggcgttcg
    14701 agttactgcc gctggcgttg accgtcaggt tgttcttgac gctcttgacg ccgtcggtct
    14761 tcttggcgat gtcggccgcc ctgatcttct ggttgccgtt ggcgaccgag ccggtgagcg
    14821 tgacaacgtc gttctccacg tcaacattga tcgtcgactc gcgcaggtcg tcggccccga
    14881 ggagctgggc gcgaaccttc gtccagagcc agcggtcatt gatgccggag ccgatggtgc
    14941 ggccgctgcg gcgggcgtca tcgtcgtagc gggcctgctc gcggttgtac tcgtcgcggg
    15001 tgatgttagc gttgacggcc cggttggtgt tggcgggcgt gggcgtggcc gtcggggtgg
    15061 cggcgcgatt ggccgtgttg gtgttgacgc ggttggcgtt ggtgttgctg tcgccgccgc
    15121 aagcggccgt gatggcgagc gccgcgaccg cgagaagcgc cgcgagtgtc cgttgattca
    15181 tgctgcctcc tgaaatgatg cttggttaac gtccgacaac ccgcaggccc ggccccggcg
    15241 gcgtgcgcgc gacccatcgc gggcgcgcgc ctgtgccctc gggagactga ctcggactct
    15301 aagggttgtg aatcttggcg ggtatattaa cgccgtccgg tgaagaggac aaggggaaag
    15361 caaggcggaa ggatgagggc ggagggatga gggatgaggg atgaaggcga gtcggcagga
    15421 ggcagcgggc agtcggcagt caaggcgctc gaccggcatt gactgctcac tgctcactgc
    15481 tcactgctta ctatcttctt tcatccctca tccctccgcc ctcatccctg ctcttacgcc
    15541 gacggccacc gtccccgttg ggaagcggtg gccgtcgatt ttggatgaac cgaacgcggg
    15601 ccgggttagg gccggttggc gttggtggcg ccggcgttcg tgttgctgcg gctcatgttc
    15661 atgttgctgc cgctcatgtt gccgttcatg ctcgaggtgt tgctgctggt gttcgccggc
    15721 ggaacgaccg tgacgttgga attggaaatc gtcacgttgg cgttcgagat ggaggtgttc
    15781 gccgggcggt tggtgttcag gttgctgacg ttggcgttac cgttgtggtt cgcgtcgccg
    15841 ccgcaagcga aggtcagggc cgcgagcgcg gtgccggcga cgagtgctgc gattttgctc
    15901 ttcttcatgt tttctcctgt gactgtctct gattttggcc tagcttttgg aaacccattg
    15961 atgtgcgccg caagacgcac gaaccctgcc gggagaggcc gggcgcgtgc gtcttacagt
    16021 gaatgatgca ataaggtcgt tgaaaaccca tctgccagcg cggcatatta cctactaatg
    16081 ttcgcaatac gcaagtgaaa aagaccacac tggggtaata aatttacaaa aaaaagaccc
    16141 ggcccagccg ccccccacgg gtctgaaagg gccaaagcgc cccctcgcgg ctccgtcgtc
    16201 accacttgac ggtgccctct ttcgtcgtgt cgatctgcgc gaggtcttgc ccggcggtga
    16261 agaagaagcc cttcgcgttg tcgaacagga aggcctgcgc gtccggctcc atgaggttca
    16321 ggccgtagtg attgatgatc atctgctgct tctggagcca ctcgctccag cactgctggc
    16381 agacctcggc ccctatcttc tgccccagct cagtcgggta gggcgcgtag cccaacgcgt
    16441 cgcgcttctg gccacagcgc gcgcacttga tctcgtcggc catcttctga aaacctcctc
    16501 cggcgggcgg cgcaggtttt cccgcacacg caaaaattcc ctcacggcag gcggataaaa
    16561 tatcacacac cgcgcccaca cgcccgcgct caggggcctt cggcggcgtc gtcgccgtcg
    16621 ctgctggccg cgcggatggc cgcgagaacg tcgtcgtcct tcggccgcag gagcgacagg
    16681 ggctgcaccc tgcgatagct gacacggccc cgcgggtcga tgacgacgac tgcgcgtgcc
    16741 gcgcgccccg gcaaccacga ggcgacgccg taaagtttga cgacgcgggc ctccgggtcg
    16801 gagagcaggc gcagggggag ccggcggttc tcggcgaagc tgcggtgttt ctccaccgag
    16861 tcggtcgaga tgccgacgac ctccgcgccc gtgcccgagt aatcctccca gcggtcgcgc
    16921 accgcgcaca actgccgcgt gcagacgggc gtctcgtcgc cggggtagaa gaggagtacg
    16981 accacacggc cgcgctgctc cgacagacgc cagtcgttgc cgtcgccgtc cggcagagta
    17041 aagtcggggg ccttctgccc cacctgcggc cctgtttgtt cgttcatgtt ttctgaaagt
    17101 cgcggatgtc tttgaaggga gtctcggggt tgttgaagtc ggggcggtag tagtgcgcgg
    17161 cgccgtcgta ctcctgaagc cagccctcct ccatgccgag ttcttcgagc gcggagacgg
    17221 cgcgcagcca ctcgcgctcg gtgatgcggc gcgagagcag gatgaagcgt tcgtcgtggg
    17281 cggccttgtt cgtcgcgtag tactgcgcca tgagcgagac ggcgacgcgc gggcttaaag
    17341 tttctctaat ccattcgagg gagtcgcgca cgcccgccac gtcgttgggc agcacgagca
    17401 gtcggacgac gaggccgcgc tgtagtagtc ccgcgtcgtc gaagacgagc cggtcgcccg
    17461 tctgccggta catctccgcg agcgcggcgc gggcgtgctc ggcgtagccg gggactttcg
    17521 agaaggcata accctcggag gaatcggcgt acttgaggtc gggcaggtag atgtccacca
    17581 cgccgtcgag gaggcggagg acttccacgg agtcgtaggc gttagtgttg tagacgacgg
    17641 gcagacgcag gcccccactg caagcgatga ggatcgcgcg ggccatctgc ggggcgaagt
    17701 gcgtggggct gacgaagttg atgttatgac agccgcgcgc ttgcagctcc agcaacatcc
    17761 cggcgaggcg ctcgtgggtg acctcgtttt tgatctgctc gcggtgggtc tgcgaaatct
    17821 ggtaattctg gcagtagacg cagcgcaggt tgcagttgcc gaagaagacg ttgccggccc
    17881 cacccgtgcc gacgagcgcc ggctcctcgc cgaagtgcgg cgtgtaggag gagacgacgg
    17941 ggaggcggcc cgagtagcag cgggcgagtt cgcccttcgt gcgattgttg ccgcagtcga
    18001 gcgggcagac ggtgcagcgc gcaagcagaa gctccagcgc ctcgacgcgc cgccgcagct
    18061 cgccgctctc atagagggct agatatgagg gtcgttgttc gttcacggcc ggcgcctcag
    18121 cgtgagccgg ttgtggtgcg ggctgtgctc gtcaacgagg tgcttggggg cgagcgtgcg
    18181 gccgagctgc cagatgaagt ccatctcggc cgtgatggtc gagagcgcga ggcgggcttc
    18241 gaggataagc aggacgctcc cccagaaaag gaagagcgag ccgaggaggc ccatcgcgac
    18301 gggcacccag gagtagcgcg cgccgtccac ggcggcgacg acgccgatgg cgacgctcgt
    18361 caggacgaag atgccgcccg ccgtatagaa gacggtcatc gagcgctgga ggatgcgggc
    18421 gcgggaggtc agcatgtcga gctggttgaa gatgacggcg cggcgctcct cgaaaagctg
    18481 cacctcgccc tcggtgcgcg ccatctcctc gaagcggtcg gagagcgagc ggacgcggtc
    18541 aacgacgcgg ccgagccgca cagaggtcga gaggatgaag gtgccgcagg cggagatgag
    18601 cacggcgggc gtgatcatcg ccgacaggac ggccagagct gaagagagtg cgtccacgtt
    18661 tcggtttccg agtttcggct tccgggggcc gcgcggcggc gcgtcgggac atttataacg
    18721 tcgcgcgcgc cggacgagca accgccgcgc gcgtgacggg tggcgcgcgc gttgcgattc
    18781 gcagatattt tctctcctca tgtgaaagtg tggttcaggc caacgtgagg tgtgttatcc
    18841 tccgcgacaa ctaataaaga ggtcttaatc taacgacatc acggcgctga agaggatgtt
    18901 ttctcttccg gcgggtgttt acttttcaca aaggagacgc tggaaatgtc tgataaagac
    18961 ttggaagaca gagacgacgc gacaggtggc ggcggcggcg aatccggcgg cctgtcagat
    19021 ggtgatgaag gctctggtgg cggcggcgac gcgccggaga cgggcgcgcg cggcggcggc
    19081 ctggccatcg gccgcgccac gggcgtcggc ggcggcggtg ctgagggcgg cgacgcgggc
    19141 ggcggaactg ccgtgggcga ggccggcggc ggcagttcga ccggcggcgg ttccggttcc
    19201 acaggtggcg gcggagcgac cggcggcggt agcagcagca gcacgtccgg cggcggtagc
    19261 agtagtacgt cgggcggcgg cagcggcggc gcgtccagcg gcggaacttc tggcggcggt
    19321 gagacaaccg gctcgggcgg tggcggaggt cgtggcggct cgcgcggcgc gtcgggcgga
    19381 ggatcgaccg gcggcgcggg tggcggcggc gcgtcgggtg gtggtcgcgg cgcgtcgggc
    19441 ggttcgtcgt cgggcggctc aggcggctct gccggaggcg ggggcagtgc gcgcagcggc
    19501 ggcacgagcg gcggcggtgg tgcgctcggt tccggcggag gcggaggcgg cttgaggagt
    19561 ggttccggtg ccgcgaagag tggcgcggcc ggaggcgcca ggagcggcag cgccggcgtc
    19621 ggcactaagg gcggcagttc taaaggtggc agtcgtgccg ggtctgccgc cgcacgcggc
    19681 ggttcaagcg ccggggccac aggtggcggc ggcggtgctc gcggtagtgg cggcggcgct
    19741 acgaagggcg cgggcgttgt gacgggcgga ggcaaggccg gggcggccag agccggcgcg
    19801 aaggccgggt caacgaaagg cggcgccaag aagggcggcg cgaccaagag cagcgccgcg
    19861 accagaggcg gcgtcgccgg tggacgcggc ggagccggcg gcggtgctgc gagaggcggc
    19921 ggtgtgggcg cgtccaaggg cggcgcgcgt tcaggcacgg cggccggacg cggcggcgcc
    19981 ggcaagggcg ccgcgaagaa aggcggcgcg tcgaagagtg gcgcgacgcg cggcggcacg
    20041 gccggtcgcg gtggcagcgg cgcggcgagt cgaggcgggg ccgctacagg cggcgccggt
    20101 ggacgcggcg gagcgggcgc gggtcgcagc ggagttgcca aaggcggtgc gcgcggaggc
    20161 gccggcggcg cgtcgaagag cggtgcggcg cgtaagggcg gcggcggtgg tcggcccgcg
    20221 ggcggcggca cggcgcgcgg cacaggcggg gggcgtggcg gctcaaaagg cgggggctcc
    20281 aaaagtggtg gctcacgccg ggggggaggc tagcggcgca gccaaaaact ctgacctgat
    20341 cacgaaagtc gccgagaccg ccggacacac ggcgg
//
LOCUS       DASVES010000394         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_746580, whole genome shotgun sequence.
ACCESSION   DASVES010000394 DASVES010000000
VERSION     DASVES010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746580"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..510)
                     /locus_tag="VF240_16160"
     CDS             complement(4..510)
                     /locus_tag="VF240_16160"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX7176799.1"
                     /translation="MSAGRADAGEDLTHAEADGGDDDNAARRSQRLDALTAVLALVGL
                     ADAVYLTAEHLSGRSVVCMVTSGCDAVLQSQYASLPGGWPLASLGALAYFAVFSLATL
                     SAFDYAHTSTLLRVVVAAMFAFTLWLLYVQAFVLEAFCSYCLLSAAVTTTLAALQVAR
                     LLLRPKNA"
     gene            complement(507..1193)
                     /locus_tag="VF240_16165"
     CDS             complement(507..1193)
                     /locus_tag="VF240_16165"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7176800.1"
                     /translation="MPLIIIGGVLLLAVVGGALLMNSNRSGSGVASGGPNTAQTQAPA
                     APRPNPLPGAEPPRAKGPESAAVTLEEFGDYQCPSCGVFNPVVNKMKATYGDRLRVVF
                     RHLPLQSIHKNAAMAARAAEAAGLQNRFWEMHDLLYESQKEWSDLPDPRPKFAEYAQR
                     AGVSDVERFKTDLESQSVSARIVADVRRASSLGISGTPSVLINGVQVRGVTESELRRE
                     IETALAGAVK"
     gene            1393..2070
                     /locus_tag="VF240_16170"
     CDS             1393..2070
                     /locus_tag="VF240_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7176801.1"
                     /translation="MSHTGSLHVRAETVQRPGADEAVSIYDRFTGLYDMMFRVNRYRH
                     SIERYLRARPLNLPAGARVLDAGCGTGLLTLALLRVLELPANITAVDLSAASLQTARR
                     ATAKEPARARHAVHFARVNALTLPFPDDSFDLVVTSGVLEYLPLAEGMHELARVIAPG
                     GYFFHLPVEPSPATRLLELMFRFKAHPPRAVHDNTVRHFRVIERHRFSPLEPISWTKK
                     AVLAQKP"
     gene            complement(2179..3159)
                     /locus_tag="VF240_16175"
     CDS             complement(2179..3159)
                     /locus_tag="VF240_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7176802.1"
                     /translation="MSRLTFMLALLLSLLPGLSFAVAQEGNARKPQPAAFASSAKLQQ
                     AVDEAAREALARFGEKGLKEKNLAITVIDLTEAQAPVSASYRGTEPIYPASVVKLFYL
                     AAAHRWLEDGRLKETEELQRALRDMIVDSSNDATHYVVDTISGVSNGAELSPREMEKW
                     AFKRNAVNRHFASLGYTVGPGALNVNQKPWCEGPYGRERVFLGKNFENRNKLTTDATA
                     RLLAEIVLGRAVTPARSARMLELMKRDFAGKSDDPDDQAHGFTGIALEPGARYWSKAG
                     WTSTTRHDAAYLELPGGRRLVIVTFTTDNAKEREVIPTVAREVIKAMNRK"
     gene            3281..4582
                     /locus_tag="VF240_16180"
     CDS             3281..4582
                     /locus_tag="VF240_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7176803.1"
                     /translation="MEQTTEAGLVRGIGKWDLVALIINSMVGAGIFVLPARAYGLIGP
                     YSLLAFIVCALIVALIILCFAEVGSRFQGTGGPYLYAREAFGPAAGFQVGWMFWLARL
                     TSSATNCNLLVVYLGYFFPTATTGGWRAAIITTVIVGLTAINYVGVRDAAVVSNLFAV
                     GKLLPLLLFIGVGFFFIAPENFNASTLPESGSFSTAVMLLVYAFTGFENASVPTGEVR
                     DPRRNMPIAILIAVGVVAVFYISIQAVCVGALGPALAASERPVADAGRVFLGAAGASL
                     IVAGAAVSIIGNLNAGVLTTPRLPFAMAERGELPAALSATHPRFHTPHVAILLTSALL
                     LALTLASSVIYALTLSTIARLLAYAATCLALPVLRRRTDAPPALFRVPGGVGVAVAAL
                     LLCVWLLSQSTGREARDAAIAVAVGLVVYAVFRLRRVRSTQ"
     gene            complement(4653..>4918)
                     /locus_tag="VF240_16185"
     CDS             complement(4653..>4918)
                     /locus_tag="VF240_16185"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7176804.1"
                     /translation="TRRTLDGKNPKGWVPEQKITVEEAVRAYTFGSAYAEFADKVKGA
                     IAPGMLADIVILDRDIFKIDANDIEKARVTLTIVDGRVVHEKK"
BASE COUNT          728 a         1665 c         1613 g          912 t
ORIGIN      
        1 gcactaggca tttttcgggc gcagcaacag gcgggcgact tgtaacgcgg cgagggttgt
       61 ggtgacggcg gccgagagca ggcaatagga gcagaacgct tcgaggacga acgcctgtac
      121 gtagagaagc cagagcgtga aggcgaacat cgcggccacg acgacgcgca gcagcgtcga
      181 ggtgtgagcg tagtcgaagg cagagagggt ggcgagcgaa aagaccgcga agtaggcgag
      241 cgcaccgagc gaggcgagag gccagccgcc cggcagagag gcgtactggc tttgcagcac
      301 cgcatcgcag ccagaggtga ccatgcagac gacgctgcgc cccgagaggt gttcagccgt
      361 caggtagacg gcgtcggcca ggccgacgag cgcgaggacg gcggtcagcg cgtcgagacg
      421 ctgcgaccgc cgcgccgcgt tgtcgtcgtc gccgccatcc gcctcggcgt gtgtcaggtc
      481 ttcgcccgcg tccgcgcggc ccgcgctcac ttcactgccc cggcgagcgc cgtctcgatc
      541 tcccggcgca gttccgattc ggtcaccccg cgaacctgca cgccgttgat gaggacggaa
      601 ggcgtgccgc tgatgccgag cgaagaggcg cggcgcacgt cggccacgat gcgcgcgctc
      661 acgctctggc tttcgaggtc ggtcttgaaa cgctccacgt cggagacccc cgcgcgttgc
      721 gcgtactcgg cgaacttcgg gcgcgggtcg ggcaggtcgc tccactcctt ctggctctcg
      781 tagagcaagt cgtgcatctc ccagaagcgg ttctgaagtc cggcggcttc ggcggcgcgc
      841 gcggccatcg cggcgttctt gtgtatcgac tggagcggga ggtgtcggaa gacgacgcgc
      901 aggcggtcgc cgtatgtggc cttcatcttg ttgacgaccg gattgaagac gccgcacgag
      961 gggcactggt agtcgccgaa ctcttcgagg gtgacggccg cgctctccgg ccccttcgcg
     1021 cgcggcggct cggcgccggg tagcgggttc ggccgagggg ccgccggcgc ttgtgtctgc
     1081 gccgtgttcg ggccgccact ggcgaccccg ctgcccgagc ggtttgaatt catgagcaac
     1141 gcgccgccga cgactgccag gaggagaacg ccgccgatga tgatcagcgg cattgagcta
     1201 tttttcttgt ttgcttggtt acgactcatc gttaaatcac caccatgttg ttcgcggggc
     1261 gcgacgcggt cggtcgcgcg cgtcttgcgc gccacgtctt atttgttatg cttaggccgc
     1321 cggctcggat acaccccttt ttcaaacggc taaaagttct accaaaacca gagaggcgac
     1381 gcggctacag gcatgagtca caccggttcc ctgcatgtac gggcggagac cgtccagcgc
     1441 cccggcgcgg acgaagccgt ctccatctac gaccgcttca cgggcctcta cgacatgatg
     1501 ttccgcgtca accgctaccg gcacagcatc gagcggtact tgcgcgcgcg cccgctcaat
     1561 cttccggcgg gggcgcgcgt cctcgacgcc ggatgcggga cgggcctgct cacgctggcc
     1621 ctgctgcgcg tgctcgaact gcccgcgaac atcacggccg ttgacctctc ggccgcgtcg
     1681 ctccagacgg cccgccgcgc caccgcgaag gagccggcgc gcgcgcgcca cgccgtccac
     1741 ttcgcccgcg tcaacgcgct caccctgccc ttccccgacg actccttcga cctcgtcgtc
     1801 acgagcggcg tgctcgaata cctgccgctc gccgagggga tgcacgagct ggcgcgcgtc
     1861 atcgcgcccg gcggctactt cttccacctg cccgtcgagc cgtcgccggc gaccagatta
     1921 ctcgaactca tgttccgctt caaggcgcac cccccgcgcg ctgtccacga caacaccgtc
     1981 cgccacttcc gcgtcatcga gcgccaccgc ttctccccac tcgaacccat cagctggacg
     2041 aagaaggccg tgctggccca aaagccgtaa atcgtcaatc gtcaatcgtg aatcgcggtt
     2101 gagtgaccgc tcggcttgag cagtgttgtc ttcaagtcgg gcggccgtca accgcatcga
     2161 tttacgattg acgatttact atttacgatt catggccttt atcacttcgc gcgcgaccgt
     2221 ggggatgact tcgcgctcct tggcgttgtc ggtggtgaag gtgacgatga cgaggcggcg
     2281 accgccgggg agttcgagat aggcggcatc gtggcgcgtc gtcgaagtcc agccggcctt
     2341 cgaccagtag cgcgcgcccg gttcgagggc gatgccggtg aagccgtgcg cctgatcgtc
     2401 ggggtcgtcg gacttgcccg cgaagtcgcg cttcatcagt tcgagcatcc gggcgctgcg
     2461 cgcgggggtg acggcgcggc ccagcacgat ctccgcgagt agccgcgcgg tggcgtccgt
     2521 ggtcagcttg ttgcggttct cgaaattttt gccgaggaag acgcgctcgc gcccgtatgg
     2581 gccttcgcac cacggcttct ggttgacgtt gagggcgccg gggccgaccg tgtagccgag
     2641 cgaggcgaag tggcggttga cggcgttgcg cttgaaggcc cacttctcca tctcgcgcgg
     2701 cgaaagctcc gcgccgttcg agacgcccga gatggtgtcc acgacgtagt gagtcgcgtc
     2761 gttcgacgag tccacgatca tgtcgcgcag tgcgcgttgc agttcctcgg tctccttgag
     2821 tcgcccgtct tcgagccagc gatgcgccgc cgcgaggtag aacaacttga cgacgctcgc
     2881 cgggtagatc ggctctgtcc cgcgatagct cgcgctcacg ggcgcctgcg cttcggtcag
     2941 gtcgatgacg gtgatggcga ggttcttctc cttcagtccc ttctcgccga agcgcgccag
     3001 cgcctcgcgc gcggcctcgt ccacggcctg ctgtagtttc gcggacgacg cgaacgccgc
     3061 cggttgaggc ttccgcgcat tgccttcctg cgcgacggcg aacgacaggc cgggcagcag
     3121 gctgagaagc agggccaaca taaacgtcag acgtgacatc aatcctttaa cctttcttaa
     3181 cgactgatat actcccgcgt gcagagggcg cgcatcactt cggtgcgcgc ccggcgcgtc
     3241 gaagaaatct tcttaacttc taacaggtga ggacatttcc gtggagcaga caacggaggc
     3301 cgggctggtt cgagggatcg gaaagtggga cctcgtcgcg ctcatcatca acagcatggt
     3361 cggggccggc atcttcgtcc tgcccgcgcg ggcctacgga ctaatcggcc cttacagtct
     3421 gctcgccttc atcgtctgcg ccctcatcgt ggcgctcatc atcctctgct tcgccgaggt
     3481 gggcagccgt ttccagggga cgggcggccc gtacctctac gcgcgcgagg ccttcgggcc
     3541 ggcggccggc ttccaggtcg ggtggatgtt ctggctcgcg cgcctcacgt cgtcggcgac
     3601 caactgcaat ctgctcgtcg tctacctcgg atacttcttt ccaacggcga cgacgggcgg
     3661 ctggcgcgcc gccatcatca ccaccgtgat cgtcgggctg acggcgatca actacgtcgg
     3721 cgtgcgcgac gcggccgtcg tcagcaacct gttcgccgtc ggcaagctcc tgccgctgct
     3781 gctgttcatc ggcgtcggct tcttcttcat cgccccggaa aacttcaacg cctcgacgct
     3841 gcctgagagc ggctcgttct cgaccgccgt gatgctgctc gtctacgcgt tcacgggttt
     3901 cgagaacgcg agcgtgccga cgggcgaggt gcgcgacccg cgtcgcaaca tgcccatcgc
     3961 catcctcatc gcggtcggcg tggtcgcggt tttttatatc tcgattcagg ccgtgtgcgt
     4021 cggggcgctc gggccggcgc tcgccgcgtc ggagcggccg gtagccgacg cgggccgcgt
     4081 gtttctcggc gcggccgggg cgtcgctcat cgtcgcgggc gcggccgtct cgatcatcgg
     4141 caacctcaac gcgggcgtgt tgaccacgcc gcggctcccc ttcgccatgg ccgagcgcgg
     4201 ggagttgccc gccgccctct cggccacaca cccgcgcttc cacaccccgc acgtcgccat
     4261 cctgctcacg tcggcgctgc tgctcgcgct gacgctcgcc agctcggtca tctacgcgct
     4321 gacgctctcg accatcgcgc ggctgctggc ctacgccgcg acgtgtctcg cgctgccggt
     4381 gctgcgccgc cgcacagacg ccccgccggc cctcttccgc gtgcccggcg gagttggcgt
     4441 ggcagtcgcc gcgctactgc tctgcgtctg gctgctctca cagagcacgg ggcgcgaggc
     4501 gcgcgacgcc gccatcgccg tcgccgtggg cctcgtcgtc tacgccgtct tcaggttacg
     4561 gagagtgcgc agcacgcagt gagtttggat tccactagcg ccgtagacgc ggctggttga
     4621 gcacttgtcc tctgctcact gctcaccgct cactatttct tctcgtggac gacgcggcca
     4681 tcgacaatcg tcaacgtgac gcgcgccttt tcgatgtcgt tcgcgtcgat cttgaagatg
     4741 tcgcggtcga ggatgacgat gtcggcgagc atccccggcg cgattgcacc tttaactttg
     4801 tcggcgaact cggcgtaggc ggagccgaag gtgtaagcgc gcacggcctc ctcgacggtg
     4861 atcttctgct cgggcaccca ccctttcggg ttcttgccgt cgagcgtgcg gcgggtga
//
LOCUS       DASVES010000395         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1623151, whole genome shotgun sequence.
ACCESSION   DASVES010000395 DASVES010000000
VERSION     DASVES010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623151"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..943)
                     /gene="sdhB"
                     /locus_tag="VF240_16190"
     CDS             complement(149..943)
                     /gene="sdhB"
                     /locus_tag="VF240_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX7176805.1"
                     /translation="MSAQTHARAAAEGAKKAPPEFVDFKIRRRDAPEKQPYWEEFRIP
                     YRPNLNIISALMEIRKNPVTRAGKQTTPPVWDMNCLEQVCGICTMVINGRVRQSCSAL
                     IDQLDQPITLEPMSKFPNVRDLVVDRQQMFDHLKRVKAWVEIDGTHDLGPGPKMSQED
                     VAERYAYSRCMTCGCCLEACPQYDGDNYIGPQAIAQVRLMNMHPTGQMSRDDRLSAIT
                     GDDGISNCGNAQNCVRVCPMSIPLTKAIYEENRETVIHGLFGWLKR"
     gene            complement(940..>2905)
                     /gene="sdhA"
                     /locus_tag="VF240_16195"
     CDS             complement(940..>2905)
                     /gene="sdhA"
                     /locus_tag="VF240_16195"
                     /inference="COORDINATES: protein motif:HMM:NF006392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX7176806.1"
                     /translation="GLAAAMKIAEAGHEVDLLSVVPVKRSHSVCAQGGINGAVNTKGE
                     GDSPFKHFDDTVYGGDFLANQPPVRDMCEMAPAIIYLFDRMGVPFSRTPEGLLDFRRF
                     GGTLHHRTAFAGASTGQQLLYALDEQVRRHEAAGLVKKYEHWEMLSLIMDDQQVCRGL
                     VAMDKSTLELRAFPADAVIVATGGPGLIFGKSTNSMVCTGSAVSACYQQGARYANGEF
                     IQVHPTSIPGEDKLRLMSESARGEGGRVWVPKQKEDKRGPSSIPESERWYFLEEKYPA
                     YGNLVPRDIATREIFQICLDGFGVGGDNQVYLDLTHIPAETLDRKLGAILEIYEMFVG
                     DDPRHVPMRIFPGVHYSMGGLWVDFKQRTNIPGLLAAGECDYSIHGANRLGANSLVSC
                     VYGGFVAAPAAIEYAKGVERNGGADDSKIYEQETKRQQELNDALVKQAGGENQYVIHD
                     EMGKWMTDNVTVVRYNDRLQATDEKLLELIDRYRHISINDSNMWATHALPHARQLQNM
                     LQLARVITLGALNRNESRGAHYKPDFPERDDDNWQKTTIAEFTGEGPVFSYEAVDVTL
                     IEPRKRDYSKGKQGAAGAKGGTAESSPTKGGAAQVPASGWDQKPENTSGPAVWGQNKE
                     QVDKSVSGDIETRREQGKELKDAGGKSDEV"
BASE COUNT          443 a          881 c          993 g          588 t
ORIGIN      
        1 gccccccgtc gccaccatca cgccggaagt ttgaaaccgg aatcgcttac gcttagggga
       61 aaccgccaca ccggcacaat cgaattgacg acaaaagagt cgccgcccgt gatacaggcg
      121 gcgactcttt tcatcagcac aaggcggctt agcgtttgag ccagccgaag aggccgtgga
      181 tgacggtctc gcggttctcc tcatagatgg ccttcgtcag cggtatcgac atcgggcaga
      241 cgcggacgca gttctgggcg ttgccgcagt tggagatgcc gtcgtcgccg gtgatggcgg
      301 agaggcggtc gtcgcgcgac atctgccccg tcgggtgcat gttcatcagg cgcacctggg
      361 cgatggcctg cgggccgatg tagttgtcgc cgtcatattg tggacaggct tcgaggcagc
      421 agccgcaggt catgcagcgc gagtaggcgt agcgttcggc cacgtcctcc tgcgacatct
      481 tggggccggg gccgaggtcg tgtgtgccgt cgatctccac ccatgccttg acgcgcttga
      541 ggtggtcgaa catctgctgg cggtctacga cgaggtcgcg gacgtttgga aatttcgaca
      601 tcggctccag cgtgattggc tggtcgagtt ggtcgatgag tgcggaacac gactggcgga
      661 cgcggccgtt gatgaccatc gtgcagatgc cgcagacctg ttcgagacag ttcatgtccc
      721 agacgggcgg cgtggtctgc ttgccggcgc gggtgacggg gtttttgcga atctccatca
      781 gggcggagat gatgttgagg ttcggccggt agggaatgcg aaactcctcc cagtagggct
      841 gcttttcagg agcgtcgcgc cgcctgatct tgaaatcgac gaactccggg ggggccttct
      901 tcgcaccctc ggcggcggcc cgtgcgtgtg tttgtgcgct catacctcgt cggatttccc
      961 ccctgcgtct ttgagttcct tgccctgctc gcggcgcgtc tcgatgtctc ccgacacgct
     1021 cttgtcaacc tgctccttgt tctggcccca caccgccggc cccgaagtgt tctccggctt
     1081 ctgatcccag cccgaggcgg gcacctgcgc cgcgccgccc ttcgtcggag agctttcggc
     1141 cgtgccgcct ttcgcgccgg cggccccctg cttgcccttc gagtagtcgc gcttgcgcgg
     1201 ctcaatcagc gtcacgtcca cggcctcgta ggagaagacc ggcccctcgc cggtgaactc
     1261 ggcgatggtc gtcttctgcc agttgtcgtc gtcgcgctcg gggaagtcag gcttgtaatg
     1321 cgcgccgcgc gactcgttgc gattgagcgc gccgagagtg atgacgcgcg cgagctgcaa
     1381 catgttctgg agctgtcggg cgtggggcag ggcgtgcgtg gcccacatgt tcgagtcgtt
     1441 gatggagatg tggcggtagc ggtcgatcag ctccagaagt ttttcgtccg tcgcctgaag
     1501 gcggtcgttg tagcgtacca ccgtcacgtt gtcggtcatc cacttgccca tctcgtcgtg
     1561 gatgacatac tggttctcac cgcccgcctg cttgacgagc gcgtcgttga gttcctgctg
     1621 gcgcttggtt tcctgttcgt agattttcga gtcgtcggct ccgccattgc gctcgacgcc
     1681 cttcgcatac tcgatggcgg ccggggcggc gacgaagccg ccgtagacgc aggagacgag
     1741 cgagtttgcg ccgaggcggt ttgcgccgtg gatggagtag tcgcattcgc cggcggcgag
     1801 caggcccgga atgttcgtgc gctgcttgaa atcgacccac agaccgccca tcgaataatg
     1861 gacgccgggg aagattcgca tggggacgtg gcgcgggtcg tcgccgacga acatctcgta
     1921 gatttcgagg atggctccga gcttgcgatc cagcgtctcg gccgggatgt gcgtcaggtc
     1981 gagatagacc tgattgtcgc cgccgacgcc gaagccgtcg aggcagattt ggaagatttc
     2041 gcgcgtcgcg atgtcgcgcg ggacgaggtt gccgtaggcc gggtacttct cttcgaggaa
     2101 gtaccagcgc tcgctttcgg ggatggagga cgggccgcgc ttgtcctcct tctgtttcgg
     2161 cacccacacg cgcccgccct cgccgcgggc ggactccgac atcagacgca gcttgtcttc
     2221 gcccggtatc gacgtggggt gaacctgtat gaactcgccg ttggcgtagc gcgcgccctg
     2281 ctggtagcag gcggagacgg ccgagccggt gcagaccatc gagttggttg acttgccgaa
     2341 gatgaggccg gggccgcctg tggcgacgat gaccgcgtcg gccggaaacg cacgcagctc
     2401 aagcgtgctc ttgtccatcg ccaccagacc acggcagacc tgttggtcgt ccatgatgag
     2461 cgaaagcatt tcccagtgct catacttctt cacgagtccg gcggcctcgt ggcggcgcac
     2521 ctgctcgtcg agcgcgtaga ggagttgctg ccccgttgag gcgccggcaa aggccgtgcg
     2581 gtggtggagc gtgccgccga agcgccggaa gtcgaggagt ccttcgggcg tgcgcgagaa
     2641 ggggacgccc atgcggtcga agaggtagat gatggcgggg gccatctcgc acatgtcgcg
     2701 cacgggcggc tggttggcga ggaagtcgcc gccgtagacc gtgtcgtcga agtgcttgaa
     2761 gggagagtcg ccttcgccct tggtgttgac ggcgccgttg atgccgccct gcgcgcagac
     2821 cgagtgggag cgtttgacgg gcacgaccga cagcagatcg acctcgtgac cggcctcggc
     2881 gatcttcatc gccgccgcga gtccg
//
LOCUS       DASVES010000396        28546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_263289, whole genome shotgun sequence.
ACCESSION   DASVES010000396 DASVES010000000
VERSION     DASVES010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263289"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VF240_16200"
     CDS             complement(<1..297)
                     /locus_tag="VF240_16200"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX7176807.1"
                     /translation="MTKATATVYVCQNCGKQSPKWLGKCPDCGEWNSFAEERARALAG
                     KAGAAAARGGVRLREVKPVAYGDIESQDDARVPSGIDEFDRVLGGGIVPGSLVLI"
     gene            complement(607..918)
                     /locus_tag="VF240_16205"
                     /pseudo
     CDS             complement(607..918)
                     /locus_tag="VF240_16205"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(986..2266)
                     /locus_tag="VF240_16210"
     CDS             complement(986..2266)
                     /locus_tag="VF240_16210"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7176808.1"
                     /translation="MSTAEAVATTEEELNPFLIATQQFDIAADYLEMDESMRQVLRNS
                     KRQLIVSIPVKMDSGETRVFEGFRVQHNIARGPAKGGIRFHPGVSLDEVKALAAWMTW
                     KCATVGIPYGGGKGGVICDPKSMSKNELERLTRRYAFEIAPIIGADRDIPAPDVYTDE
                     QTMAWIMDTISMVRGHTELGVVTGKPISLGGSQGRAEATARGCLYVLREACRVRGVNL
                     SGARVAVQGFGNAGANIARLMAEDGARVIAVCDSGAGVHNPQGLDVQAALRHKAETGT
                     LKGAPNSKPLDRCDDVLEVECDILLPSALENAITLSNVERVNTKIIAELANGPVTPGA
                     DRVLFDRGVFLIPDILANAGGVTVSYYEWVQDQYSFFWPEKQVNDTLEQTMRRAFEDV
                     YATAQRYKTDMRTGAYILAVGRVAEATRVRGIFP"
     gene            2534..3355
                     /locus_tag="VF240_16215"
     CDS             2534..3355
                     /locus_tag="VF240_16215"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7176809.1"
                     /translation="MFRNLVESGSHATDIKRRGSFFAAAVAFYAILLTAAGIGSIYAV
                     NVRLDDREELEVLALMSFPPQPATAEPVRRDAPRPAGGGQTTQAARRPEHSAIIPYDG
                     TREVASAGTREVSPTLRNIEIGADNGIDPAHVAGPIRTGVPGGLRTDGPVVADDGDIV
                     APPARTPVKVEPPKPETPRVLRLASSIISSKAINKPKPDYPAIAIRMHVEGTVPVQIL
                     IDEQGRVVQAQATSGHPLLQKAAADAARRATFSPTFLSDKPIKASGVIYYNFVIQ"
     gene            complement(3474..4142)
                     /locus_tag="VF240_16220"
     CDS             complement(3474..4142)
                     /locus_tag="VF240_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7176810.1"
                     /translation="MIKLLTSLIVVLVSAGGARADVITFFDFNDSNLTSDRGQSANIT
                     TNFGATAFAAGSTVNADPDGAGPELASTAGQALNLVGSANNGNHIQFGVSTLSLASIQ
                     ISFAAQRSSTGFNNNELLFSADGGTSFTSAGFFIPAATFGLLAFDLSGFAALDNQAEV
                     IFRIVFNGASSSTGTNRIDNIAVTGTGAAEPVPEPVSVMMLGTGLASAAWSIRRRGKG
                     VGRS"
     gene            complement(4407..5576)
                     /gene="alr"
                     /locus_tag="VF240_16225"
     CDS             complement(4407..5576)
                     /gene="alr"
                     /locus_tag="VF240_16225"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7176811.1"
                     /translation="MSGGDYNFEGRRPTWAEVDLDALASNYRVVRRHVGAGVKVLAVV
                     KADAYGHGAAECARRLEAEGADWFGVASPEEGVELRRAGVTRPVLSFGGFWEGQAETC
                     IGERLAPAVYRLDMAAALDEAARVSGVVADVHVKIDTGMGRLGVRYEGAAEFAAKLAG
                     FKNIRVDGLLTHFAVADEPERLCFTDDQVKRFRDALEAFRAHGHRPAHEHMANSAATF
                     AHQQTWGNMVRPGGVLYGLWRDVLPAGSRDPGLRPVMSVRSRVTLLKRVRRGETLGYG
                     CTFEASRDMMVATLPIGYHDGYMRALSNRGRVIVRGTYAPVVGRVSMDLTLIEVTDVA
                     GAAEGDVVTLLGTDGALGVPAEDLAQTAGTISYEVTCGVSGRVPRLHVPIDKRGV"
     gene            complement(5716..8331)
                     /gene="dnaB"
                     /locus_tag="VF240_16230"
     CDS             complement(5716..8331)
                     /gene="dnaB"
                     /locus_tag="VF240_16230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX7176812.1"
                     /translation="MTRYPVDSARDQMLERPLPHSAESERAILGAIILDNGMVNQAIE
                     LLRPEDFYIRANQFVFRAMISLSERGAEINPILLGEELRREGVLEQTGGLAYVSELTY
                     GLPHFTNLAHYGKIVHDKSLLRQLVKVSNKITSEALEEEDEASHILDHAEQMIFALAD
                     ERTRQGFAHVKPIADQLLEKVQEMSGRTTVITGLATGFKELDEMTSGLQPADLIIVAA
                     RPSMGKCLAFDSEIVMADGGVATIEEVYRVRGARLLTLGDDFKFAVARPSDYVDDGVK
                     PVFRVATRLGRVVETTLTHPFLTLDGWQPLSRLKAGDRIAVPRVLSIFGEGEMRECEV
                     KLLAYLLGDGCLRGASPVFTVGKPALRADFETAVANFGGVETKQANSKDRTFSLRVRK
                     AKGYKENALTAWLRGLGVYGCDSHSKFIPGGVFRLKREQLALFLNRLFSTDGWATVLS
                     SGQSQLGFASVSERLARQVQHLLLRFGVIATLKKRAVKYKGGRRVAWQLDITDGRSIR
                     TFIEEIGIHGKEAALASVTRALSARRYQTNRDLVPVEVWGRVATLKGGESWASLARRA
                     GLRGRTNIHVGQRALSRNRLLALGETLGSVELRCLAESDVYWDEIVSIEFAGEKQVYD
                     LTVPATHNFVANDICVHNTSLALTLAQNAAIIAGAVVGVFSLEMSKESLVMRMLCSEG
                     RVDAHRFRGGYLHREEWARLAGALGTLADTRIYIDDTPGISVLEMRAKARRLAAEQKR
                     LDLIVVDYLQLMSGSSRRIESRQQEVSLISRELKGLAKELNVPLVALSQLSRAPEARS
                     DHRPQLSDLRESGSIEQDADLVSFIFREEQYNRTEENAGMAELIIAKQRNGPTGTVKL
                     AFLKEFTRFENMWRE"
     gene            complement(8511..9107)
                     /gene="rplI"
                     /locus_tag="VF240_16235"
     CDS             complement(8511..9107)
                     /gene="rplI"
                     /locus_tag="VF240_16235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX7176813.1"
                     /translation="MAQTTVLLREDVDNLGARGEIVKVKAGYARNYLLPRKLAVKATA
                     GNVKQIEAERGALLKKEARERSTAEGQAALMNALRLEFERKVGEHGILYGSVTSMDIA
                     EALKAKGYEIDRRRITLREAIKETGEFSVGVRLHRDVTVEIPVIVTGEGGAQTAGAAA
                     AAAPAAEAATPAADEGESASEEPAAAASDEANENSPVE"
     gene            complement(9126..9335)
                     /gene="rpsR"
                     /locus_tag="VF240_16240"
                     /pseudo
     CDS             complement(9126..9335)
                     /gene="rpsR"
                     /locus_tag="VF240_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(9603..9995)
                     /gene="rpsF"
                     /locus_tag="VF240_16245"
     CDS             complement(9603..9995)
                     /gene="rpsF"
                     /locus_tag="VF240_16245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX7176814.1"
                     /translation="MAEKRIYEIIYIVDTDTTEEETARLTETLRQTITDQGGVIVKDE
                     SMGRRNLAYRIGRKTEGIFMFFEVEGTGREIAELERRMRVMDQVIRYLTVRVDEDRQR
                     AEKFKARRALRASKRPGATAAAGAGSRE"
     gene            complement(10152..10721)
                     /gene="pth"
                     /locus_tag="VF240_16250"
     CDS             complement(10152..10721)
                     /gene="pth"
                     /locus_tag="VF240_16250"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX7176815.1"
                     /translation="MLVVGLGNPGAQYEWTRHNLGFMLVDRAARAAGREVKRLECRAL
                     VGRAEIEGRAVELVKPQTYMNLSGESVACLLRKRAGLTPSIGLLVVCDDIAIPFGSVR
                     LRPRGSSGGQKGLKNIIDVLGADEFARLRIGIRPEHPVGDTARFVLENFSKAQRAELE
                     TILDRSADALRAVLRDGIDKAMAQYNGDK"
     gene            complement(10782..11462)
                     /locus_tag="VF240_16255"
     CDS             complement(10782..11462)
                     /locus_tag="VF240_16255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX7176816.1"
                     /translation="MAERKEITLRAQLREGRGKNDAGRLRRSGLVPLTVYGDGNDAVS
                     AAAPLAELAAILRSDTGHNTIFTLDIEGVGESEVLFLHRQIDPVRGRLIHADLRRLVR
                     GQKIEVTVSLHLDGDPVGVREGEGILEQLLREVQVRCAPREIPEALHVDVSNLGVHEV
                     LHVSDIQLTENVEILTDPETAVATVAVLREEPVEEVAVEEEAVEPEVIGKGKKEDEGE
                     GEREKGKG"
     gene            complement(11680..12627)
                     /locus_tag="VF240_16260"
     CDS             complement(11680..12627)
                     /locus_tag="VF240_16260"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7176817.1"
                     /translation="MSTTGGIKVFSGNANRALTNEICNYLSCDPGVATSARFSDGEYN
                     FQITENVRGADVFIVQPTCPPTDAHLMELLVMLDAFRRSSAERVTAVIPYYGYARSDK
                     KDRPRVPIASKLVANLITTAGADRVLTIDLHAAQIQGFFDIPVDHLYAAPVFIGYYQE
                     NGLPNLTVVAPDTGGAERARAYAKRLDAELALCDKRREKANEAEVMNVVGDVRGRNCL
                     IVDDMCDTGGSLTKVAKALREAGADRIHACFTHPVLSGRACEQLKGSDIEMTVTTNTI
                     PLRGEACNLDNVRVLSIAPLLAKAIKSIHEETSVSSLFV"
     gene            complement(12713..12784)
                     /locus_tag="VF240_16265"
     tRNA            complement(12713..12784)
                     /locus_tag="VF240_16265"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12750..12752),aa:Gln,seq:ttg)
     gene            complement(12799..13728)
                     /gene="ispE"
                     /locus_tag="VF240_16270"
     CDS             complement(12799..13728)
                     /gene="ispE"
                     /locus_tag="VF240_16270"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7176818.1"
                     /translation="MSDDRFTLPAFAKINLQLRVLGRRDDGYHEIETVFQTITLRDRL
                     TFEPLDDGRIELSCDTAEVPADESNLVHRAALALRQRYGVQAGARVTLEKNIPAQAGL
                     GGGSADAAVALLGLTRLWKLAPGLTELAELGARLGADVAFFLTGGTALGTGTGATITA
                     LEDAPARHLVVVTPGVKVSTAEAYKALNAPALTKAESAANLSVSRAESGISGSICEAL
                     RNDFEAVVFRLHPETGRAREALLAAGARDALLSGSGSSVFGFFDSKGDAERARGALGP
                     ETGWRVFACATLSRAEYLRALGRCAGLLSGAGP"
     gene            complement(13798..14445)
                     /locus_tag="VF240_16275"
     CDS             complement(13798..14445)
                     /locus_tag="VF240_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7176819.1"
                     /translation="MSDKSCLPAQAHPLLLVALVAFAALPVSANETNTKPAARPVARL
                     VSTSRDEVASRPRPRVIANYRAVPAATTSPRPAATSAASPTRSESRLLDLINEERRAH
                     GRRPLAWDGALTRMARHHSENMSRQGFFSHVDRDGLSMTGRAELLGVHGWKALAENLA
                     YNQGYDDPESYAVEGWMHSEKHRANILKGEFTHAGLGVARGADGRIYFTQVFMER"
     gene            complement(14778..16676)
                     /locus_tag="VF240_16280"
     CDS             complement(14778..16676)
                     /locus_tag="VF240_16280"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176820.1"
                     /translation="MRQSSRTTARAWSRASVLAPALLLAVSAAPSVSAQNANSPLAPP
                     PASSAQGASSGANTPDARQTDGPIAAPTRERRAQAYAKLLEGQRHYSAARTGSLTMDG
                     LRSAQSAFQQAADLDPTLSEARTALAEIAFFFLNDLPLAEREASAAVRINRDNVGAHR
                     VLSRVHALQSKLAEATPERASAERAVAELREIVRLRPGDAEAWALLGEFYFALGRDDE
                     AAEAFRRWAGAPAPIDVRFFQVITKGRELTPDAANARLAETLLRLGKTQEAAVAARQA
                     WMMSPENPAHLQLFILASAQLAQAHADESRFDEAIKIYEDLLKARGVGDTLLADRSVR
                     RFAAPLLGEVALLRQRAGQAAEASAMIERMRRVLGAEDPAPDVYAVDLLREQGKSGEA
                     LEAARTARRRLPDDAQLVYREARLLAESGRVDEAASLYRGRLKGAPTDYDEYLSLASV
                     LITGGRGREAADAARKALELVPANQDELTVRALLLLSSAQDRAGDFKGSEESLRQILS
                     KDPDNVAALNNLGYFLTERNERLGEALEMIQRAVRAAPGNASYLDSLGWVYFKLGKLE
                     EAERYLRDAARRNPNSAAIQEHLGDLFNSRGMAEQARAAWQKALSLSTEPADIIRIKT
                     KLGDGASR"
     gene            complement(16673..17209)
                     /locus_tag="VF240_16285"
     CDS             complement(16673..17209)
                     /locus_tag="VF240_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176821.1"
                     /translation="MSRNSESDSVVEDELHGRHRAAVLTIYAVLALTVLLAALAFAGV
                     FSDKVGFDPVIDGALRISVVFFGLGAVALRRAMFSGTRLQAIASLRGPSGLLAHLQKT
                     TMQVAAIAAAIAVMGFALTLLRGGAAGETPAMVWIGLIAVAVLLYAYPRRSAWRRVLQ
                     SAREGEVDDASSAKGTTA"
     gene            complement(17415..19052)
                     /gene="bamD"
                     /locus_tag="VF240_16290"
     CDS             complement(17415..19052)
                     /gene="bamD"
                     /locus_tag="VF240_16290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7176822.1"
                     /translation="MRIRNLMLSAVGAAVLWAAPSAFAVVHAQPAGGAAQSNLTPAQR
                     LAVLRSKLEQTRRTLNSSIAGLNAKDAGKDAEDARARLSGLEKEVASLLGDVQDMRGR
                     LDRAERYEVSDVDKLEAAATDLDTRVQSGLRATISERQAYTGTAETKKKKKGGGFFSK
                     IFPFGGGGDEDKYSELIGSVAPGRDRVLFEEATKEARKSNYETARALYSVIISTYPDS
                     PYLQLSKLAIGDTFYLEGETSALIQASSAYQDWLSFFPTHPLADDVMLKMAEVEMRRM
                     GLPDRDISNAKKAEQRIKVLLQQFPQTSLRPDAELRLREVQEVLAKHNHMVGDHYWDR
                     YRRGVSPNPKGAQSRYREIVEKTPHYSGMADVLAKLAQTYLDEEEPDEAAKYLQQIAR
                     YHPNSSHVERAREQLAAIGVPAPDPDPAYINREEPEGPGFFSGLKLELLGTTPKTIEK
                     KGILIGGSKSGDLIDEAIKNGGILPATVPTVPVKRTDPGRDVRPLPAANPPATAPTNT
                     PTIQPTQPGPPKEGSNPNRPPATQPTQPATPPPGDKP"
     gene            complement(19162..19824)
                     /gene="rpe"
                     /locus_tag="VF240_16295"
     CDS             complement(19162..19824)
                     /gene="rpe"
                     /locus_tag="VF240_16295"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7176823.1"
                     /translation="MIEIAPSILSADFARLGAEIEAAERGGATLVHVDVMDGHFVPNI
                     TIGLPVVRSLARATRLPLDAHLMIEEPGRYAEQFAEAGAAMVSVHVEADPHLHRTLAG
                     IRKQGAKAGVVLNPATPVAALEEAIEFADYVLVMSVNPGFGGQQFIRAATEKVRRLKR
                     MIDERGLPTRIEIDGGIDRETIAEVAAAGAEIMVAGTAVFGQGDPERAVRELLAASVQ
                     MA"
     gene            complement(19869..20525)
                     /locus_tag="VF240_16300"
     CDS             complement(19869..20525)
                     /locus_tag="VF240_16300"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX7176824.1"
                     /translation="MLGLILCAFAFGLAGTVYLSLRSPSVEVPDVTNKTFNEGESALE
                     TTGLSIRERARRYKSDVRPGVILDQFPRAGAVVKQGQTVAVIVARAPKEGEAPSSDEI
                     VEGEEKKAEAETAAAVANENQNRPRRKTNTNKNTNANVNANNRNSNASTGGSNANAGG
                     TQNANRTTAPNANRNANSARNGNTNDRPGRNTNNRSASTTGARNANSTRNANGNKRPN
                     "
     gene            complement(20693..22105)
                     /gene="rsmB"
                     /locus_tag="VF240_16305"
     CDS             complement(20693..22105)
                     /gene="rsmB"
                     /locus_tag="VF240_16305"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX7176825.1"
                     /translation="MSDERKGRGARKGGGVGGPVAEGRAAAVSPARWAAFGILRRVEE
                     EGAFAAPLLANLADSMRADDRALCYELVLGVLRRQLWLDKLLEHYAGRDAQTLDAPVR
                     HALRLGLYQLRFLTRVPARAAVNESVNLAHAARLHSAASFINAVLRRAGREPDYDPAA
                     AVADAVERAAVETSHPAWLIARWADAFGRQEAFDFARANNAHAPVSFRLNPLRAEASE
                     VIDKLRAAGVRVEPSQIAPGGWRMEESGDASPLLRAWADDGSLYMQDEASQLVAHVLG
                     ARAGERVLDVCAAPGSKTTHVAALAGGRAHVVAGDIHAGRLRLLVELATRQGLKGIRP
                     VALDAESPLPFADASFDRVLVDAPCTGAGTLRHNPEIRWRIRPSDILELSARQLAILH
                     GAARVVRPGGRLVYSTCSVEPEENEGVAAAFLGGVVDFRQVEAAPAPARLLLNTGAAR
                     TWPHRDGTDGFFVAAFERLA"
     gene            complement(22113..23054)
                     /gene="fmt"
                     /locus_tag="VF240_16310"
     CDS             complement(22113..23054)
                     /gene="fmt"
                     /locus_tag="VF240_16310"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7176826.1"
                     /translation="MRLIFMGTPASAVPSLRRCVEDGHEVVAVWTQPDRPAGRGNRLH
                     VPPVKEFALSRGLTVRQPEKIRTDAAFDLFTHRTVDAVVVVAYGRILPPAYLRAPLRG
                     CVNVHFSLLPKYRGAAPVNWAVVRGENESGVTTMLMDEGLDTGPILLQRATPIGAGET
                     APELLARLAEDGAELLSQTLAQFAELEARPQDESAATHAPILRREDGLIDWGMSAADI
                     ERRVRGFQPWPNAHTTLQGRRLVVLKSEVVEEFEEGASHTPRPGEVVEARGDRLIVAC
                     GGGTSLRLFDVQPEGKRRMDARDFINGTHVAVGEKLG"
     gene            complement(23175..24104)
                     /locus_tag="VF240_16315"
     CDS             complement(23175..24104)
                     /locus_tag="VF240_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176827.1"
                     /translation="MSKQQHSLTARARLAAALTLLLACFSGAHAQQAGGAKGAMIFAV
                     SGEMGTTIDPVVLVSGGKFVSPPMDESGDDPLAKFAGEYYREGRKFRIISGGGEAGSL
                     TVKRSHVADECFRTGADVELESPVKIGRVRLALATDSDTLGRGQGTRRAPTEAERAAA
                     LKLAEESMRRRRVPAAVLKTLGAINLTATDLDGDGRAELIGSFVAKQGKQSRHLLFLI
                     ALPQADTFKTGLARYETVVAKDLPDPSVIDEVGNGGFLSEILVDQVDLDGDRVGEVVT
                     TGASFEGQWYYIYKRGRGGWSRAYESSNYRCAY"
     gene            complement(24236..25825)
                     /gene="pruA"
                     /locus_tag="VF240_16320"
     CDS             complement(24236..25825)
                     /gene="pruA"
                     /locus_tag="VF240_16320"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX7176828.1"
                     /translation="MQNEFRNESFTDFSGEENASAMREALERVRGELGRDYPLVIGGE
                     RIMTGRTFESFNPANKTQVVGRFQKATEELARLAVERASETFKTWSRTPAEERAALLF
                     RVADLLRERRHQMSAWMVYEVAKTWAEADGDTAEAIDFCDFYAREMLRYAGGQPLVKI
                     EGEENRLEYIPLGVGVVIPPWNFPLAIMAGMTVASIVTGNTVVLKPSSDAPTIAYKFF
                     ELLEEAGVPPGVVNFTTGSGAEVGDVIVDHPKTRYVAFTGSKEIGLRINERAAKVNAG
                     QIWIKRVVAEMGGKDAIIVDAGVDLDEAATGVVQSAFGFQGQKCSACSRAIIHTQVYD
                     SMIEKIVERTERLKLGDPNAPGTSLSAVINNKAFKSINEYIERGKADGGRVVAGGGAD
                     GEQGFFIEPTVIADVKAGDTIEQQEIFGPVLACIRARDFDDALAIANDTEYGLTGAVY
                     TNDEEKLERARREFHVGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAGGRDYLGLFMQ
                     AKVVSEKIRPGASDKEKRTAI"
     gene            complement(26070..26585)
                     /gene="def"
                     /locus_tag="VF240_16325"
     CDS             complement(26070..26585)
                     /gene="def"
                     /locus_tag="VF240_16325"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7176829.1"
                     /translation="MALLKIVKYGDPVLEQSGDPVTEFDDGLKKLVGDMFETMYSASG
                     VGLAAPQVGLSKRLFVMDCSGGDDPGARVALINPVVLRVEGDQTGDEGCLSFPGIYFN
                     VKRSLRAVVRAQDINGEEFEYDGLELEARCMLHETDHCDGIVFIDHTTPLKRELVKRK
                     IKKLAKAGKWV"
     gene            complement(26830..27861)
                     /locus_tag="VF240_16330"
     CDS             complement(26830..27861)
                     /locus_tag="VF240_16330"
                     /inference="COORDINATES: protein motif:HMM:NF045090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HEX7176830.1"
                     /translation="MGFRLSKSIRLGKFFRLNLSRSGIGGSVGVGPLRVGTGPRGPRL
                     SVDLPGAGVSYVKHLGSSGGGRRTRVAHALPAAPPSAPEVVTQETPEPGFFAPGHEKD
                     FVEGLNELGAGRADTALQHFLSAADEEPGAAVYAASILSRHTEGRARAVEMLERLVQS
                     DAELPTPLMRKYLPGVEAPVQITPSVRASVALDELAVALLLVEFYQEQGRVDDATGLL
                     EEVADLDPEPALTLSLCELYADAGAWDGIIDRAQGVEVSDDLTLEIVIFYGRAMQEKG
                     LHEAAVSVFTGALRKKKGRSPALLREAVYRRALSYLKLGRAAQANRELQKLYAEDPTF
                     RDVERLIQG"
     gene            complement(28109..>28546)
                     /locus_tag="VF240_16335"
     CDS             complement(28109..>28546)
                     /locus_tag="VF240_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7176831.1"
                     /translation="TETATFSQSFVRVGLMPDGGSTFFLPRLVGRTRAFELMATGATV
                     KAEEALRLGLVNRVVAANELEAVVGELAARMAAAPQPSLARIKGALSRAEQEELAAAL
                     EYEADGQADCFRSADFREGVAAFMQKREPVFGKHKTSAAKGDE"
BASE COUNT         4635 a         9424 c         9334 g         5153 t
ORIGIN      
        1 gatgagaacg aggctgccgg gcacgatgcc gccgccgagc acgcggtcga actcgtcgat
       61 gccggagggg acgcgcgcgt cgtcctgcga ctcgatgtcg ccgtaagcga ccggcttcac
      121 ctcgcgcaga cgcacgccgc cgcgcgccgc ggccgccccc gccttcccgg cgagtgcgcg
      181 cgcccgctcc tcggcgaacg aattccactc gccacagtcg ggacacttcc ccagccactt
      241 gggagattgt tttccgcagt tctgacagac gtaaacggtc gccgttgcct tcgtcaatgc
      301 tgttaaatcc cttctctgat aaagcagtct tcgcgggtat aaaactcacg cccaacttct
      361 aaattaacgc ctgtatttta acattttgcc ggccgattgg ccgttcgacg tggccgcggt
      421 tttgaccttc atgttgaata ccgggacagg tgattcaccg cagaggcgca gagggcgcag
      481 aggggccgca gagggtcgaa tggctcactc cgcgtcgagt gaatcgttca agcctgcggc
      541 gtagtctctg cgtcctctgc gcctctgcgg tgagtccaac ttacattacg ctcaagccga
      601 gagcactcac tccccttcca acaaagcctt gatgttctcc gtcggccgac cgagcacggc
      661 gcgcgctcca cgttcgacga tgggccgctg aagcaggtca ggatactcga ccatgagccg
      721 aataatctca tcgtcggaga gttcgcgttt gccgagtccg agttctttat agacagcctc
      781 gctcgtccgc agcagctcgc gcggcgccag ccccatcttc ttgatcaact cgcgcagctt
      841 tccctctcca agcggctcga cgtagtagtt gaccttcccg aaatcgaccc cgctctcacg
      901 gagcaggcga tgcgcctcgc ggcacttggt tcaagtcggc ttctcataga cggtaatctt
      961 ctcagacata aattgctctc attccctatg ggaaaatccc gcgcacgcgc gtcgcctcgg
     1021 ctacccggcc gacggcgagg atgtaggcgc cggtgcgcat gtcggtcttg taacgctgcg
     1081 cggtggcgta cacgtcttcg aaggcgcggc gcatcgtctg ctccagcgtg tcgttgacct
     1141 gcttctcggg ccagaagaag gagtactgat cctgcaccca ctcgtagtag ctgacggtga
     1201 cgccgccggc gttggcgagg atgtccggga tcaggaagac gccgcggtcg aagagcacgc
     1261 ggtcggcgcc gggggtgacg gggccgttgg ccagctcggc gatgatcttc gtgttgacgc
     1321 gctcgacgtt cgagagcgtg atggcgttct ccaaagccga cgggagcaga atgtcgcact
     1381 cgacttcgag cacgtcgtca cacctgtcaa gcggcttgga gttcggcgct cccttgagcg
     1441 tgccggtctc ggccttatgt ctgagcgccg cctgcacgtc gagaccctgc gggttgtgga
     1501 cgccggcccc cgaatcgcag acggcgatga cgcgcgcccc gtcttcggcc atcagccgcg
     1561 cgatgtttgc cccggcgttg ccgaagccct gcacggcgac gcgcgcgccc gagagattga
     1621 cgccgcgcac gcggcacgcc tcgcgcagca cgtagagaca cccgcgggcg gtggcctcgg
     1681 cgcgcccctg cgagccgccg agcgagatgg gcttgcccgt gaccacgccc agctccgtgt
     1741 ggccgcgcac catcgagatg gtgtccatga tccaggccat cgtctgctcg tcggtgtaca
     1801 cgtcgggcgc cgggatgtcg cgatccgccc cgatgatggg cgcgatctcg aaggcgtagc
     1861 ggcgcgtgag ccgttccagc tcgttcttcg acatcgactt cggatcgcag atgacgccgc
     1921 ccttgccgcc gccgtaaggg atgccgacgg tcgcacactt ccacgtcatc cacgcggcga
     1981 gcgccttgac ctcgtcgagc gagacgccgg ggtggaagcg gatgccgccc ttggcggggc
     2041 cgcgcgcgat gttgtgctgg acgcggaagc cctcgaagac gcgcgtctcg ccgctgtcca
     2101 tcttgacggg aatggagacg atgagctgcc gcttggagtt gcgcaagacc tgccgcatcg
     2161 actcgtccat ctccagatag tcggccgcga tgtcgaactg ctgcgtggcg atcaggaagg
     2221 ggttgagttc ctcctcggtg gtggccaccg cttcggctgt gctcatgggc gtgattcctc
     2281 tccgggactt tcggtggatg ggtaagcctt aaacttcagg cgcgtgcggc acatcctaga
     2341 agaaaatagt tcaaagttca aagttcaaag ttcaaagtca acagactcca catgcgcttt
     2401 gaactttgaa ctttgaactt tgaacttaaa gtcggacttt gaactatttt ggaacaggaa
     2461 catcgcgcgc ggagtctaaa gttgtggaag ctcccggacc cgattggcac cggcaagagg
     2521 gaggacattg gagatgttta ggaatctggt cgagtccggc tcacacgcca cggacatcaa
     2581 gcggcgcggc tctttcttcg ccgccgccgt cgccttctac gccatacttc tcacggccgc
     2641 cggcatcggc agcatctacg ccgtcaacgt ccgcctcgac gaccgcgagg agcttgaggt
     2701 gctggcgttg atgagctttc cgccgcagcc cgcgacggcc gagccggtgc gacgcgatgc
     2761 gccacggccc gcgggcgggg gtcagacgac gcaggccgcc cggcgtcccg aacactccgc
     2821 catcatccct tacgacggaa ccagagaagt cgccagcgcc ggcacacgtg aggtcagccc
     2881 cactctcagg aacattgaaa tcggcgccga caacggcatc gacccggcgc acgtggccgg
     2941 ccccatccgc acgggcgtgc cgggcggctt gagaacggac ggccccgtcg tcgccgacga
     3001 cggagacatc gtggccccgc ccgcgcggac tcccgtgaag gttgagccgc cgaagccgga
     3061 gacgccgcgc gtgctgcggc tggcgagttc gatcatcagc agcaaggcca tcaacaagcc
     3121 gaagcccgac tacccggcca tcgccattcg gatgcacgtg gagggcaccg tccccgtgca
     3181 gattctgatc gacgagcagg gacgcgtcgt tcaggcgcag gccacgagcg ggcacccgct
     3241 gctccagaag gccgccgccg acgccgcgcg ccgcgccacc ttctcaccca cgttcctgtc
     3301 ggacaagccc atcaaagctt ccggcgtcat ctactacaac ttcgtgattc agtagaccgc
     3361 aaaggcccgg gcgggccggc aacgtcctta tccacgctgt cggcccgccc cccgccacgt
     3421 ttcccggcgc ggggggcgcc tccgccctcg ccgcgtgcgc ggggccgtcc cgttcaactg
     3481 cggccgacgc ccttcccgcg gcggcggata gaccatgccg cgctcgccag ccccgtgccc
     3541 aacatcatca ccgacaccgg ctccgggacg ggttccgccg cgcccgtgcc cgtgaccgcg
     3601 atgttgtcta tccggttcgt gccggtcgag ctcgaggccc cgttgaagac gatgcggaag
     3661 atgacctcgg cctgattgtc gagcgccgcg aaccccgaga ggtcgaaggc caacagcccg
     3721 aaggtcgccg ccgggatgaa gaacccggcc gaggtgaagc ttgtccctcc gtccgcgctg
     3781 aacagcagct cgttgttgtt gaatccggtg ctgctcctct gagccgcgaa cgaaatctgg
     3841 atcgatgcca agctcagcgt gctgacgccg aactggatgt ggttcccgtt gttggcgctg
     3901 ccgaccagat tgagcgcctg gccggccgtc gaggcgagtt cggggccggc cccgtcgggg
     3961 tcggcgttga cggtgctgcc cgccgcgaac gcggtcgcgc cgaagttggt ggtgatgttc
     4021 gcgctttggc cccggtccga agtcaggttc gagtcgttga agtcgaagaa ggtgatgaca
     4081 tcggcccgcg ccccgccggc cgacaccaac acgacgatca gactggtgag gagtttaatc
     4141 atgagtttgc ctctcttcct tttaggttgc tgggtttttg agtgtgtgcg cgccgacaat
     4201 ccgggtggac ggatcgccgg agcggctgcg aattgagaac cgcacccaac cttgtaagcc
     4261 ctttggtcga ggctgccgcg ccggacggac acacctccgc gcccgtcatg cagagcaggt
     4321 tgttagaatt gaaaaggctc ggcccgccac ttgaggttgt gaggattgcg ggccgctcgc
     4381 cactaaatcg ctgggaagca ataccgctaa acgccccgct tgtcaatagg gacgtggagg
     4441 cgcggcaccc ttccgctcac gccgcaggtg acttcatagg aaatcgtgcc ggcggtctgc
     4501 gcgaggtctt ccgcggggac gccgagcgcg ccgtcagttc cgagcagcgt caccacgtcg
     4561 ccctccgcgg ctcccgccac atccgtgacc tcaattagcg tcaggtccat cgagacgcgc
     4621 ccgaccacgg gggcgtatgt gccacgcacg atcactcgac cccggttcga gagggcgcgc
     4681 atgtagccgt cgtggtagcc gatgggcagc gtcgcgacca tcatgtcgcg cgaggcttcg
     4741 aaggtacaac cgtagccgag cgtctcgccg cgccgcacgc gctttaagag cgtgacgcgc
     4801 gagcgcaccg acatcacggg ccgcaggccg gggtcgcgag agccggctgg cagcacgtcg
     4861 cgccacagcc cgtagagcac gccgccgggc cgcaccatgt tgccccacgt ctgctgatgg
     4921 gcgaacgtcg cggccgagtt cgccatgtgc tcgtgcgcgg gacggtgtcc gtgcgcgcga
     4981 aaggcttcga gcgcgtcgcg gaaacgcttc acctgatcgt ccgtgaagca caggcgctcc
     5041 ggctcgtccg ccacggcgaa atgcgtcaac agaccatcaa cacgaatgtt cttaaacccc
     5101 gcgagctttg cggcgaactc cgccgccccc tcgtagcgca cgccgagccg gcccatcccc
     5161 gtgtcgatct tgacatgcac gtccgcgacc acgcccgaca cgcgcgcggc ctcgtcgagc
     5221 gcggcggcca tgtcgagccg gtagacggcc ggcgccaaac gctcgccgat gcacgtttcg
     5281 gcctgcccct cccagaagcc gccgaacgaa agcaccggcc gcgtcacgcc cgcgcggcgc
     5341 agctcaacgc cttcctccgg cgaggccacg ccgaaccagt ccgccccttc ggcctccaga
     5401 cggcgcgcgc actcggccgc gccgtgcccg taggcgtcag ccttcaccac cgccaacacc
     5461 ttcacgcccg cgccgacgtg ccgtcgcacc acgcggtagt tcgacgccag cgcgtcgagg
     5521 tcaacttccg cccacgtcgg gcgtcgccct tcaaaattat aatcgccgcc cgacatcgtc
     5581 acctcacatc gaccacagac tcacaagccc gacctcgccc attgtgctcc gccttcaaca
     5641 caaaacaaaa ccgccgacga acggcggtca cacatcaact tcaatatcga atcgtaaaca
     5701 aacccttcgc gcgtcttact cgcgccacat gttttcaaac cgcgtgaact ctttcaggaa
     5761 cgccagcttc accgtccccg tcgggccgtt gcgctgcttg gcgatgatca gttcggccat
     5821 ccccgcgttc tcctccgtcc ggttgtactg ctcctcgcgg aagatgaagc tcacgaggtc
     5881 ggcgtcctgc tcaatcgatc cgctttctct caagtccgag agctgcggcc ggtggtcgct
     5941 gcgcgcttcg ggagcgcgcg atagttgcga gagcgcgacg agcgggacgt tcagctcctt
     6001 ggccagcccc ttcaactcgc gcgagatcag cgacacttcc tgctggcgcg actcgatgcg
     6061 gcgcgacgac cccgacatca gttgcaggta atcgacgacg atcaggtcga gccgcttctg
     6121 ctcggccgcg agccgacggg ccttggcgcg catctccagc acggagatgc cgggcgtgtc
     6181 gtcgatgtag attctcgtgt cggccagcgt gccgagcgcg ccggcgagtc gcgcccactc
     6241 ctcgcggtgg aggtagccgc cgcggaagcg gtgcgcgtcc acccgcccct ccgagcacag
     6301 catcctcatc accagcgact ccttcgacat ctccagcgag aacaccccga caaccgcccc
     6361 cgcgatgatc gccgcgttct gcgccagcgt cagcgccaga ctggtgttgt ggacgcagat
     6421 gtcgttggcg acgaagttgt gcgtcgccgg aacggtgagg tcgtagacct gcttctcacc
     6481 cgcgaactct atggagacaa tctcgtccca gtaaacgtcg ctctctgcca ggcatcgaag
     6541 ctcgacactt cccagtgtct cgccgagagc aagcaggcgg ttgcgcgaaa gagcgcgctg
     6601 cccgacgtgg atgttcgtgc gaccgcgcag gcccgcccgg cgcgcgagcg acgcccatga
     6661 ctctccacct ttgagagtgg caacgcgccc ccagacctcg acggggacga ggtcgcgatt
     6721 cgtctggtat ctcctcgcgg aaagcgcgcg ggtgacagat gcgagcgccg cctctttccc
     6781 gtggatgccg atctcttcga tgaacgtcct gatcgaacgc ccgtcagtga tgtccaactg
     6841 ccaggcgact cgccgcccgc ctttatactt aactgcgcgc ttcttcagcg tggcgatgac
     6901 gccgaaccgc aggagcagat gttgaacctg acgcgcgagc ctttcactta ccgaagcaaa
     6961 gccgagttgc gactgcccgc tgctcagaac agttgcccag ccgtcggtcg agaacagtcg
     7021 gttcaggaac agcgccaact gttctctctt gagcctgaaa acccctccgg ggataaactt
     7081 cgagtgactg tcacagccgt agacgcctaa cccccttagc caagcggtga gcgcgttctc
     7141 cttataccct ttcgcctttc ggacgcggag cgagaaagtg cggtctttgc tgttcgcctg
     7201 tttcgtttca acaccgccga agttcgccac cgccgtctcg aagtccgcgc ggagcgcagg
     7261 cttcccgacc gtgaagacgg gcgaagcgcc tcgcaggcac ccgtcgccga ggagataggc
     7321 cagcagcttc acctcgcact cgcgcatctc gccctcgccg aagatgctca acactcgcgg
     7381 cacggcgatg cggtcgccgg cctttaatcg cgagaggggt tgccacccgt ccagagtcag
     7441 gaaggggtgc gtcaaagtgg tctccaccac cctcccaagc ctcgtggcca cgcggaagac
     7501 gggcttgacg ccgtcgtcca catagtcgga cgggcgcgcc acggcgaact taaaatcatc
     7561 gccgagggtg agcagccgcg ccccgcgcac gcggtagact tcctcaatcg tggccacgcc
     7621 gccgtcggcc atgacgatct cgctgtcgaa ggccagacac ttgcccattg acgggcgggc
     7681 ggcgacgatg atgaggtcgg cgggctggag gccggaggtc atctcgtcga gttctttgaa
     7741 gccggtggcg aggccggtga tgacggtcgt gcggcccgac atctcctgaa ctttttcgag
     7801 cagttggtcg gcgatgggct tgacgtgggc gaagccctgt cgggtgcgct cgtcggcgag
     7861 ggcgaagatc atctgctcgg cgtggtcgag gatgtggctg gcttcgtcct cctcttcgag
     7921 cgcctctgag gtgatcttgt tcgagacctt gacgagttgg cgcaggagcg atttgtcgtg
     7981 gacgattttg ccgtagtgcg cgaggttggt gaagtgcggc agaccgtagg tcagctcgga
     8041 gacgtaggcg aggccgccgg tctgttcgag cacgccctcg cgacgcagct cctcgccgag
     8101 gaggatgggg ttgatttcgg cgccgcgctc actcaaagag atcatcgcgc ggaagacgaa
     8161 ctggttggcg cggatgtaga agtcttcggg gcggaggagt tcgatggcct gattgaccat
     8221 cccgttgtcg aggatgatcg ccccgaggat ggcgcgctcg ctctcggcgc tgtggggcag
     8281 cgggcgttcg agcatctgat cgcgcgcgga gtcaaccgga tatcttgtca tcgggagacc
     8341 gaacgattcg cgtctactgc ggagaacaaa agaccggccg cgcccgccgg ccggagtcga
     8401 gacaaaatcg accgcggcgc tgatttcgac gtggcgcgag ccggtgctct tatacaacag
     8461 cgcgaacgca cgcgacaaca gtcatgttga cgcgcgcgcc cgaccgagtt ttattccaca
     8521 ggtgagtttt cgttcgcctc gtcggacgct gcggccgccg gctcttccga cgcgctctcg
     8581 ccttcgtcgg cggcgggcgt tgcggcttcg gcggcgggcg cggcggcagc ggcggcgccc
     8641 gccgtctgcg cgccgccctc gcctgtgacg atgacgggaa tctccaccgt cacgtcgcgg
     8701 tgcagtcgca cgccgacgct gaactcgccc gtctccttga tcgcctcgcg cagcgtgatg
     8761 cgccggcggt cgatctcgta gcccttagcc ttgagggctt cggcgatgtc catcgaggtc
     8821 acggagccgt agaggatgcc gtgctcgccg accttgcgct cgaattcgag ccggagggcg
     8881 ttcatcagcg cggcctggcc ttcggcggtc gaacgctcgc gcgcctcctt cttcaagagc
     8941 gcgccgcgct cggcttcgat ctgcttgacg ttgccggccg tcgccttgac ggcgagcttg
     9001 cgcggcaaca ggtagttgcg cgcgtagccg gccttgacct tcacgatctc gccgcgagcg
     9061 ccgaggttgt ccacgtcttc acgtaacagg actgtcgtct gtgccatctc tggtgtcctc
     9121 ctcccttatt ccgccgcgta ggggatgagc gcgatgttgc gcgcgcgctt gatggcctcg
     9181 gccagcatcc gctggtgagg cgcgcacgtg cccgagatgc gccgcgggag aatcttgccg
     9241 cgctccggga tgtagctggt gagcagcttc acgtccttgt agtcgatgag atcgacctta
     9301 tcgagacaga agcggcacac cttgcggcgc cggaagcgcc cacctccgcg accgcctccg
     9361 ccgcggcggt cgtcgcgccc gcccgatggc tggtgaaccg gcccgtcgtc gtctgtttca
     9421 aaatctccgt cgtagtttct cgacataatt acactctcaa cttttctctt gggcgaccgc
     9481 ctcgcctgtc ccgcaccgtg ccggggcagg agaccgagcg ccgccaggtc agccgtcaaa
     9541 ttccgtttcg gccacacgcg ccaagggacg cttactgaaa accgttccct cggcgaccgg
     9601 ccctactcgc gcgacccggc ccccgcggcg gcggtggcgc ccggtcgctt cgacgcgcgg
     9661 agcgcgcggc gcgccttgaa cttctcggcg cgctgacggt cttcgtccac gcgcaccgtc
     9721 aggtagcgaa tcacctgatc catgacgcgc atgcggcgct ccagctcggc gatctcgcgg
     9781 cccgtcccct cgacctcgaa gaacatgaag atgccttcgg tcttgcgccc gatgcggtag
     9841 gcgaggttgc ggcgccccat gctctcgtcc ttgacgatga cgccgccctg atccgtgatc
     9901 gtctgccgga gcgtctccgt cagacgcgcc gtctcctcct cggtcgtgtc cgtgtcaacg
     9961 atgtaaatga tctcgtaaat tctcttctct gccaaaacca tcctccttct ggatgtctag
    10021 ccccgcccgc gggcgaagca aggaagaatt aaggagtgat aagtgatcag tgatgagtga
    10081 taagagctat tagcttaaag ctgttagcta acaactaaga gcattgcttt cttcttatca
    10141 cttatcactc atcacttatc accattgtat tgcgccatcg ccttgtcgat gccgtcgcgg
    10201 aggacggcgc ggagcgcgtc ggcgctccgg tccaaaatcg tttcgagttc ggcgcgctgg
    10261 gccttcgaga aattctcgag gacgaagcgc gccgtgtcgc ccaccgggtg ctcgggccgg
    10321 atgccgatgc gcagtcgcgc gaactcgtcg gcgccgagca cgtcgatgat gttcttcaag
    10381 cccttctggc cgccgctcga cccgcgcggg cggagccgaa ccgagccgaa gggaatcgcg
    10441 atgtcatcgc aaacgaccag cagaccaata gacggcgtaa ggcccgcgcg cttacgcagg
    10501 aggcaggcga ccgactcgcc gctcaggttc atgtacgtct gcggtttgac gagttcgacg
    10561 gcgcggccct cgatctccgc cctgccgaca agcgccctgc actccaaccg tttgacctcg
    10621 cgccccgccg cccgcgccgc ccggtcaacg agcatgaagc cgaggttgtg gcgcgtccac
    10681 tcgtactgcg cgcccggatt gccgaggccg acgaccagca taagcaccgg ggaagggcga
    10741 aggggaaaag ggcaaggggc ggaggcgaat ttctcctgcc cttacccctt ccccttttcc
    10801 ctttcccctt ccccttcgtc ttccttcttg cccttcccga tcacctcggg ctccacagcc
    10861 tcttcctcga ccgcgacctc ctcgaccggc tcctcgcgca ggacggcgac cgtggcgacg
    10921 gccgtctccg ggtcggtcag gatttcgacg ttctccgtca actggatgtc ggagacgtgg
    10981 agaacctcgt ggacgccgag gttcgacacg tcaacgtgaa gggcctcggg gatttcgcgc
    11041 ggcgcacaac gaacctgaac ctcgcgcagg agctgttcga ggatgccctc tccttccctg
    11101 acgccgacgg ggtcgccgtc aagatgcagc gacaccgtga cctcgatctt ctggccacgc
    11161 acgagccggc gcagatcggc gtgaatcaga cggccgcgga cggggtcgat ctgccggtgg
    11221 aggaagagca cttcactttc gccgacgccc tcgatgtcga gcgtgaagat cgtgttgtgg
    11281 cccgtgtcgg agcggagaat cgcggccagc tcggcgagcg gcgcggcggc cgacacggcg
    11341 tcgttgccgt caccatagac ggtcaacggc accagccccg accgacgcag tcggcccgcg
    11401 tcgttcttgc cccggccctc gcgcagttgc gcgcgtaacg taatttcttt tctctctgcc
    11461 atactcactc ccaatgaaaa aagcgttcag ccttcggcga tcagcaaaca gctaacgagg
    11521 tcactcgtca gcggcccgtt gtccgttgtt catttccgcg gacttgaaag tctcaaggcc
    11581 gaggaacgaa aacaactgac cactgaccat tgactacgga cagcctttag ctgatagctg
    11641 accgctgact gctgaacgct tctcagtttt tgcccttcgt cagacgaaga gcgaagagac
    11701 gctcgtctcc tcgtggatcg acttgatcgc cttcgccaga agcggtgcga tgctcaaaac
    11761 cctgacgttg tcgagattac acgcctcgcc ccggagcggg atcgtgttag tcgtcaccgt
    11821 catctcgatg tccgaaccct tcaactgctc gcacgcgcgg cccgagagca ccgggtgcgt
    11881 aaagcacgcg tgaatcctgt cggccccggc ctcgcgcaaa gccttcgcga ctttcgtcag
    11941 cgagccgccc gtgtcgcaca tgtcatcgac gatgaggcaa ttcctgccgc gcacgtcgcc
    12001 gaccacgttc atcacctcgg cctcgttggc cttctcacgg cgcttgtcgc acagcgccag
    12061 ctcggcgtcg agccgcttgg cgtaggcccg cgcgcgctcg gccccgcccg tgtcgggcgc
    12121 caccaccgtc agattcggca gcccgttctc ctggtagtag ccgatgaaga ccggggcggc
    12181 gtaaaggtga tcgacgggga tgtcaaagaa gccctgtatc tgcgccgcgt ggaggtcgat
    12241 ggtcagcacg cgatccgcgc cggccgtcgt aatcaggttc gccaccagct tcgacgcgat
    12301 gggcacgcgc ggccgatctt tcttgtccga gcgcgcgtag ccgtagtatg gaatcaccgc
    12361 cgtcacgcgc tcggccgagg agcggcggaa ggcgtcgagc atcacgagca gctccatcag
    12421 gtgcgcgtcc gtcggcgggc acgtcggctg cacgatgaaa acgtcggccc cgcgcacgtt
    12481 ctccgtgatc tgaaagttgt actcgccgtc cgagaagcgc gccgacgtgg cgacgccggg
    12541 gtcgcaactc aggtagttgc aaatctcgtt cgtcagcgcg cggttcgcgt tgcccgagaa
    12601 gactttgatg ccgccggtgg tgctcatctt tgagtgatga gtgatgagtg atgagtgata
    12661 agagtaagaa agctgtcttt cttatcactt atcactgatc actgatcact agctggggag
    12721 gtaggactcg aacctacgaa tgccggttcc aaagaccggt gccttaccac ttggcgactc
    12781 cccaattccg ttcacgtctc acggccccgc tcctgagagc agacccgcgc accggccgag
    12841 cgcacgcaag tactcggccc gcgagagcgt ggcgcacgcg aagacgcgcc agcccgtttc
    12901 cgggccgagc gccccgcggg cacgctccgc atcccccttg ctgtcaaaga aaccgaagac
    12961 gctcgagccg ctgcccgaga gcaacgcgtc gcgcgcgccc gccgccagaa gcgcctcacg
    13021 cgcccgcccc gtttcggggt ggagtcggaa gactacggcc tcgaaatcgt tccgcagcgc
    13081 ctcacagata gaacccgaaa ttcccgactc tgcgcgcgaa acagacaggt tagcggcgct
    13141 ctcagccttt gtcaaggcag gtgcgttcag cgccttgtag gcttccgcgg tcgagacttt
    13201 gacgcccgga gtgaccacga ccagatgccg cgcgggcgcg tcttcgagcg ccgtgatggt
    13261 cgcgcccgta cccgtcccga gcgccgtccc gccggtcaga aagaaggcca cgtccgcacc
    13321 gagacgcgcg cccagctcgg ccagttcggt cagcccaggc gcgagcttcc acaggcgcgt
    13381 gaggccgagg agcgcgacag cggcgtccgc cgaaccgcct ccgagtccgg cctgcgcggg
    13441 gatgttcttt tcgagcgtga cgcgtgcgcc ggcctgcacg ccgtagcgtt ggcgcagggc
    13501 gagcgccgct ctgtggacga ggttgctctc gtccgccggc acttcggccg tgtcgcagga
    13561 gagttcgatt cgcccgtcgt caagcggctc gaaggtcagg cggtcgcgaa gcgtgatcgt
    13621 ctggaagacg gtctcgatct cgtggtagcc gtcgtcgcgc cgcccaagga cgcggagttg
    13681 gaggttgatc ttggcgaagg ccgggagggt gaacctgtcg tctgacataa gaagagcggc
    13741 cggcgctact ttccagcgcc ggccgcccga agattacacc gtcctcgcga ggaagcgtta
    13801 gcgctccatg aagacctgcg tgaaatagat gcgaccgtcg gccccgcgcg cgacgccgag
    13861 gcccgcgtgc gtgaactcgc ccttcaggat gttggcccgg tgcttttcag agtgcatcca
    13921 gccctcgacg gcgtaagact ccgggtcgtc gtagccctga ttataggcga ggttctcggc
    13981 gagcgccttc cagccgtgga cgcccagaag ctctgcccgg ccggtcatgc taagcccgtc
    14041 gcgatccacg tggctgaaga agccctgccg cgacatgttc tccgagtgat gacgcgccat
    14101 gcgtgtcaaa gcgccgtccc aggcgagcgg cctgcggcca tgcgcccgac gctcctcgtt
    14161 gatcaaatcc agtagacgac tctcgctccg tgtcggggac gccgccgacg tggccgccgg
    14221 ccgcggagac gttgtcgccg ccggcacggc gcggtagttg gcgatgacgc gtgggcgcgg
    14281 gcgcgaagcc acctcatcgc gggaggtgga gaccagacgc gcgacgggcc gcgctgccgg
    14341 cttcgtgttc gtctcgttgg ccgagaccgg aagcgcggcg aaggccacga gcgcgacgag
    14401 caggaggggg tgcgcctgcg cgggcagaca acttttgtca gacaaccggg ggaaactcat
    14461 tggggtaaag cacctctgac ttcaaatgtc ggcagagagg gcgccttttt cagcaactga
    14521 ccgttggttg gggctaacgg ccaatcgcgt tgtcaatatt acaactcggt ttaaaccccg
    14581 tcaatctcta atacacggag actgccggct catcccgagc ctaaatcata accccttgtg
    14641 gtctaccatt tagccggttg gatgggtggt caagcctgta gcgtccggga gaacaggggg
    14701 gatggacttc tacaaagatt gactgcccga aggaaggacg gacgtaatct acacacaccc
    14761 cggcccggag gcaagggtta tctactggcg ccgtcgccta acttggtctt aatcctgatg
    14821 atgtcggccg gttctgtcga gagggaaagg gctttttgcc acgcggcgcg ggcctgttcg
    14881 gccatcccgc ggctgttgaa aaggtcgccc agatgctctt gaatcgccgc cgagttcggg
    14941 ttgcggcgcg cggcgtcccg tagataccgc tcggcctctt ccagtttgcc gagtttaaaa
    15001 tatacccagc ccagactgtc gagatacgag gcgttacccg gcgcggcgcg gacggcgcgc
    15061 tggatcatct ccagcgcctc gccgaggcgt tcgttgcgct ccgtgaggaa gtagccgagg
    15121 ttgttgaggg cggccacgtt gtcggggtct ttggaaagaa tttgacgcag agactcctcg
    15181 gagcctttga aatcgccggc gcgatcctga gcggacgaga gcagcaggag cgcgcgcacg
    15241 gtaagctcgt cctgattggc cgggacgagt tcgagcgcct tacgcgccgc gtcggcggcc
    15301 tcgcgcccgc gtccacccgt gatcaagacg ctcgccagcg aaaggtattc gtcgtaatcc
    15361 gtcggcgcgc ccttgagccg cccgcgatag aggctcgcgg cctcatcgac gcgccccgac
    15421 tcggcgagca gacgggcttc tctgtagacg agttgtgcgt cgtcggggag tcggcgccgc
    15481 gccgtgcgcg cggcctcaag ggcctcgcca cttttgccct gctcgcgcag aagatcgacg
    15541 gcgtaaacgt ccggcgccgg gtcttcggct ccgagcactc gacgcattcg ctcaatcatc
    15601 gccgacgctt ccgctgcctg tccggcgcgc tgccgcagga gggcgacttc gccgagcagt
    15661 ggggcggcga agcgcctcac gctcctgtcg gcgagcagcg tgtcgccgac tccgcgggcc
    15721 ttgagcaggt cttcgtaaat tttgatggct tcatcgaaac gcgactcgtc ggcgtgggcc
    15781 tgcgccagtt gtgccgaggc gaggatgaag agttgcaggt gggcgggatt ttccggggac
    15841 atcatccacg cctgacgcgc ggcgacggcc gcctcttgcg tttttccgag acgcagcaac
    15901 gtctcggcga ggcgcgcgtt ggccgcgtcg ggcgtgagtt ccctgccctt ggtgatgacc
    15961 tgaaagaagc gcacgtcgat gggggccggt gcgcccgccc agcggcggaa cgcttcggcc
    16021 gcctcgtcgt cgcgcccgag ggcgaagtaa aactctccga ggagcgccca cgcttcggcg
    16081 tcgccggggc gcaggcgaac tatctcacgc agttccgcaa cggcgcgctc ggcgctcgcg
    16141 cgctcaggcg tggcttcggc gagtttgctc tggagcgcgt ggacacgcga gaggacgcgg
    16201 tgcgcgccca cgttgtcgcg gttgatgcgg acggcggccg acgcctcgcg ctcggccagc
    16261 ggaaggtcgt tgagaaagaa gaaggcgatc tcggcgagcg cggttcgcgc ctcggagagc
    16321 gtggggtcaa ggtcggccgc ctgctggaag gcgctctgcg ccgagcgcag gccgtccatc
    16381 gtgaggctgc cggtgcgtgc ggcggagtaa tgacgctgcc cttcgagcag cttggcgtaa
    16441 gcctgcgcgc gccgctcgcg ggtcggcgcg gcgatggggc cgtcggtctg ccttgcgtcg
    16501 ggcgtgttgg cgccagagct tgcgccctgc gcggaggagg cgggcggagg cgcgagcggg
    16561 ctgttcgcgt tctgcgcgga gaccgagggc gcggccgaga cggccagcag caacgccgga
    16621 gccaggacgc tcgcacgaga ccacgcgcgt gctgtcgttc gcgaagactg cctcaagctg
    16681 ttgtcccttt cgcggacgac gcgtcgtcca cctcgccctc acgtgctgat tgcaatacgc
    16741 gccgccacgc cgagcggcgc gggtaggcat agagcagcac ggcgacggcg atgagaccga
    16801 tccagaccat cgcgggcgtc tcgccggcag cgccgccgcg gaggagcgtc agtgcgaagc
    16861 ccatcacggc gattgcggcg gcgatggccg cgacctgcat cgtcgtcttt tgcaggtgcg
    16921 cgagcagccc ggaggggcct cgcagggagg cgatggcttg caggcgtgtg cccgaaaaca
    16981 tcgcgcgccg cagggccacg gctccgaggc caaagaagac gacggagatg cggagcgccc
    17041 cgtcgatgac ggggtcgaag ccgaccttat ccgagaagac gccggcgaag gcgagcgcgg
    17101 cgagcaggac ggtcagggcg agtacggcgt agatggtcaa caccgccgct cggtggcggc
    17161 cgtgtagctc gtcttcaaca acgctgtcgc tttcgctgtt ccggctcaca ccttcacctc
    17221 ttccgcgtag atttcggact tgtctctcag atgtctgtcg tggcgggggg tgcgaaggat
    17281 attaacaccc gccctgttta ccggcaaagt tttcgcggcg ggcgttcatg cgtgtaaaga
    17341 aagaggcgac cgactcgctt attcggagcc ggtcgcctga agggttcaca cagtttctta
    17401 cgcgcgccgt gccgttacgg cttgtcgccg gggggcggcg tggccggctg cgtcggctgc
    17461 gtcgcgggcg ggcggttcgg gttgctaccc tccttgggcg ggccgggctg cgtcggctga
    17521 atcgtgggcg tgttggtggg ggccgtggcc ggagggttcg ccgcgggcag cggtctcacg
    17581 tcgcggccgg ggtcggtgcg cttgacgggc acggtgggca cagtcgcggg gagtatgccg
    17641 ccgttcttga tggcctcgtc gatgaggtcg ccggatttgc tgccaccgat gaggatgcct
    17701 ttcttctcga tggtcttggg agtggtgccg aggagttcga gtttgaggcc gctgaagaag
    17761 cccggccctt ccggctcctc gcggttgatg taggccgggt cggggtcggg cgcgggcacg
    17821 ccgatggccg ccaactgctc gcgcgcgcgt tcgacgtggc tgctgttcgg atggtagcgg
    17881 gcgatctgct ggagatactt cgcggcctcg tccggctctt cctcgtcgag ataggtctgg
    17941 gcgagtttgg cgagcacgtc ggccatgccg ctgtagtgcg gggtcttctc gacgatctcg
    18001 cggtagcgcg actgcgcgcc cttcgggttc ggcgagacgc cgcggcggta gcgatcccaa
    18061 tagtggtcgc cgaccatgtg gttgtgcttg gccagaactt cctgcacctc gcgcaggcga
    18121 agctcggcgt cgggccggag gctggtttgc gggaactgct ggagcagaac cttgatgcgc
    18181 tgctcggcct tcttggcgtt cgagatgtcg cggtcgggca ggcccatgcg acgcatctcg
    18241 acctcggcca tcttgagcat cacgtcgtcg gccagcgggt gcgtcgggaa gaacgacagc
    18301 cagtcctgat aggccgacga cgcctggatg agcgcgctcg tctcgccttc gaggtagaag
    18361 gtgtcgccga tggcgagctt ggagagctgg aggtaaggcg agtccgggta agtcgagatg
    18421 atgacgctgt agagagcgcg ggcggtctcg tagttggact tgcgcgcctc cttcgtcgcc
    18481 tcctcaaaga gcacccggtc gcggcccgga gcgacgctgc cgatcaattc ggagtacttg
    18541 tcctcgtcgc cgccgccgcc gaacgggaag attttgctga agaagccgcc gcccttcttt
    18601 ttcttcttcg tctcggctgt ccccgtgtaa gcctgtcgct cgctgatcgt ggcgcgcagg
    18661 cccgactgca cgcgcgtgtc gaggtctgtg gccgccgcct ccagcttgtc aacgtcgctc
    18721 acttcgtagc gctcggcgcg gtcgagccgg ccgcgcatgt cctgcacatc tccgaggagg
    18781 gaggcaacct ccttctccag cccgctgagg cgcgcgcgcg cgtcctcggc gtccttgccg
    18841 gcgtccttcg cgttgaggcc ggcgatggag ctgttgagcg tgcgccgcgt ctgttcgagc
    18901 ttcgagcgca agactgcgag gcgctgcgcc ggcgtcagat tactctgcgc cgcgccgccg
    18961 gccggctggg cgtggactac ggcgaaggcc gacggcgccg cccacaacac cgccgcgccc
    19021 acggccgaca gcatgagatt gcgaattcgc attagtagaa cctccaaaaa caagtgataa
    19081 gtgatgagtg ataagtgatg agagaaggca tctgtttttt cttttcttat cacttatcac
    19141 tcatcactca tcaccttctt ttcaagccat ctgcaccgag gccgcgagga gttcgcggac
    19201 ggcgcgctcg gggtcgccct gaccgaagac ggcggtgccg gcgaccatga tctcggctcc
    19261 ggcggcggcc acctcggcga ttgtttcgcg gtcgatgccg ccgtcgattt cgatgcgcgt
    19321 cggaagcccg cgctcgtcga tcatgcgttt gaggcggcgc accttctcgg tcgccgcacg
    19381 gataaactgc tgcccgccga agccggggtt gaccgacatc acgaggacgt agtcggcaaa
    19441 ctcgatggcc tcctccagcg ccgcgacggg agtggccggg ttgaggacga cgccggcctt
    19501 cgcgccctgc ttgcggatgc ccgcgagcgt gcggtgcagg tgcgggtcgg cctcgacgtg
    19561 aacggagacc atcgccgcgc ccgcctcggc aaactgctcg gcgtacctgc ccggctcttc
    19621 gatcatcaga tgcgcgtcga ggggcaggcg ggttgcgcgc gccagcgagc ggacgacggg
    19681 caaaccgatg gtgatgttgg gcacgaagtg gccgtccatc acgtccacgt ggacgagggt
    19741 cgcgccgccg cgctcggcgg cctcgatctc ggcgccgagg cgcgcgaagt cagcggacaa
    19801 aatcgagggc gcgatctcta tcacaggttc gctctccgaa tcactttcaa tttgagacgc
    19861 cgccccgctc agttggggcg tttgttgccg tttgcgttgc gcgtcgagtt ggcgttgcgc
    19921 gcgccggtcg tgctggcgct gcggttgttc gtgttccggc cgggacggtc gttcgtgttg
    19981 ccgttgcggg cggagttggc gttgcggttc gcgttcgggg ccgtggtgcg gttcgcgttc
    20041 tgcgtgccgc cggcgttagc gttcgagccg cccgtgctgg cgttcgagtt gcggttgttg
    20101 gcgttgacgt tggcgttcgt gttcttgttc gtgttggtct tgcggcgcgg gcggttctgg
    20161 ttttcgttgg cgacggcggc ggccgtctcc gcctcggcct tcttctcctc gccctcgacg
    20221 atctcgtcgg acgagggcgc ctcgccctct ttgggcgcgc gcgcgacgat gacggcgacc
    20281 gtctgcccct gcttgacgac cgcgcccgcg cgcgggaact ggtcgaggat gacgccgggt
    20341 ctcacgtccg acttgtagcg cctggcccgc tcgcggatgc tcaagcccgt ggtctcaagg
    20401 gccgactcgc cttcgttgaa agtcttatta gtaacgtcgg gcacctcgac cgaggggctg
    20461 cgtagcgaaa ggtagaccgt gccggccagc ccgaacgcga aggcgcacag gatgaggccg
    20521 agcataccga tctttccgag tgccgcgaga atcgttcgca aagtaaattg tgccgggtgg
    20581 tcaagcagtc gcgggcgcat gcggcgtgtg cggcgcgcgc ccggcagcgg ttacaagcgg
    20641 cgaagtttaa cacccgcttg tcaggggccg caaggcactc gggcggcgac ctttaagcga
    20701 ggcgctcgaa ggcggcgacg aagaatccgt ccgtgccgtc gcggtgcggc caggtgcgcg
    20761 ccgcgcccgt atttaagagc agccgcgcgg gcgcgggcgc cgcctccacc tgtctgaaat
    20821 caacgacgcc gcccagaaag gccgccgcca cgccctcgtt ctcctccggc tcaaccgagc
    20881 aggtcgaata gactaaacgc ccgccggggc gcacgacgcg cgcggccccg tgcaaaatcg
    20941 ccagttggcg cgcggacaat tccaaaatgt ccgagggtct gatgcgccag cgaatttcgg
    21001 ggttgtgccg gagcgtgccg gcgccggtgc agggggcgtc aacgagcacg cggtcgaagg
    21061 aggcgtcggc aaagggcagc ggcgattcgg cgtcgagggc gacggggcga atgcccttca
    21121 gcccctgccg cgtggcgagt tcaacgagga ggcggaggcg gcccgcgtgg atgtctccgg
    21181 cgacgacgtg ggcgcggcct ccggcgagcg cggcgacgtg cgtcgtcttg ctgcccggcg
    21241 cggcgcacac gtcgagcacg cgctcgcccg cgcgcgcgcc cagaacgtgc gcgacgagtt
    21301 gcgacgcctc gtcctgcatg tagagcgaac catcatcggc ccacgcgcgc agcaggggcg
    21361 acgcgtcgcc cgactcttcc atacgccagc cgccgggcgc gatttgcgac ggctcgacac
    21421 ggacgccggc cgcgcgcagc ttgtcgatca cttcgctcgc ttcggcgcgc agcgggttga
    21481 gtcggaaaga gacgggcgcg tgtgcgttgt tggcgcgggc gaagtcgaac gcttcctgcc
    21541 ggccgaacgc gtcggcccag cgcgcgatga gccacgcggg gtgtgaggtc tcgacggccg
    21601 cgcgctcgac cgcgtcggcg accgcggcgg ccgggtcgta atccggctcg cggccggcgc
    21661 gacggagcac ggcgttgatg aacgacgcgg cggaatgcag acgcgcggcg tgcgcgaggt
    21721 tgaccgactc gttgacggcc gcacgcgccg ggacgcgtgt gagaaagcgg agttgataca
    21781 gccccagcct cagggcgtgg cggacgggcg cgtcgagagt ctgggcatcg cgcccggcat
    21841 agtgctcaag gagtttgtcg agccagagtt gtcggcgcag cacgccgagc acaagctcgt
    21901 agcagagggc gcggtcgtcg gcgcgcatcg agtcggcgag gttcgcgagc agcggcgcgg
    21961 caaacgcgcc ctcttcttcg acgcgtcgca gaatcccgaa cgcggcccaa cgcgcgggcg
    22021 acaccgccgc tgcacgcccc tccgcgaccg gcccgccgac gccgcctccc ttccgcgcgc
    22081 ccctcccttt tcgttcgtca ctcaaaattt cgtcagccca acttctcgcc gacggcgacg
    22141 tgcgtgccgt tgataaaatc gcgcgcgtcc atgcggcgct tgccctcggg ctgaacgtcg
    22201 aacaggcgca gcgacgtgcc gccgccgcag gcgactatca ggcggtcgcc gcgcgcctcg
    22261 accacctcgc cgggcctcgg cgtgtgcgac gcgccttcct caaactcttc gacaacttcc
    22321 gacttcagga cgacgaggcg tcggccctga agcgtggtgt gtgcgttcgg ccacggttgg
    22381 aagccgcgca cgcgccgctc gatgtcggcg gcgctcatgc cccagtcgat gaggccgtcc
    22441 tcgcggcgaa ggatgggcgc gtgtgtcgcc gcgctttcgt cctgcggtcg cgcttcaagc
    22501 tcggcgaact gcgcgagcgt ctggctcaaa agctcagcgc cgtcctcggc gagccgcgcg
    22561 agcagttccg gcgcggtctc gcccgcgcca atcggcgtcg cgcgctgcaa gagaatcggc
    22621 cccgtgtcga gaccctcgtc catgagcatc gtcgtcacgc cggactcgtt ctcgccgcgc
    22681 acgacggccc agttgacggg cgcggccccg cgatacttcg gcagcagcga gaagtggacg
    22741 ttgacgcagc cgcgcagggg cgcgcgcaag taagcgggcg ggagaatgcg cccgtaggcg
    22801 acgacgacga cggcatccac cgtccgatgt gtgaagaggt cgaaagccgc gtcggttcta
    22861 atcttttcgg gttggcggac agtgaggccg cggctgaggg caaactcctt gacggggggg
    22921 acgtggagac ggttgccgcg gcccgccggc cggtcgggct gcgtccagac ggcgacgacc
    22981 tcgtgcccgt cctcgacgca gcgccgcagc gacggcaccg ccgacgcggg cgtgcccatg
    23041 aagatcagac gcataagagc tgttagcggt tagctattag ctgtaagcca gatgcgagca
    23101 gcgaggtctg tgttcagcga cggcgcgcca gccggcgcat gccgtgagct aacagctact
    23161 agctaacagc ttccctaata cgcacacctg tagtttgagc tttcgtaagc cctgctccag
    23221 ccgccgcggc cgcgtttgta gatgtagtac cactgaccct cgaagctggc cccggtggtg
    23281 acgacttccc ccacccggtc gccgtcaaga tcaacttgat cgacgaggat ttcggagagg
    23341 aagccgccgt tgcccacctc gtcgatgacg ctcgggtcgg gcaagtcctt cgccacgacc
    23401 gtctcgtaac gcgcaagccc ggtcttgaac gtgtcggcct gcggcaacgc gatcaggaag
    23461 aggagatgac gcgactgctt gccctgcttg gcgacgaagc tgccgatcaa ttcggcgcgg
    23521 ccgtcgccgt cgaggtcggt cgccgtcagg ttgatggcgc cgagcgtctt gagtacggcg
    23581 gcggggacgc ggcggcggcg catcgactct tccgcgagct tcagagccgc ggcgcgctcc
    23641 gcttccgtgg gcgcgcgccg cgtgccctgt ccgcggccga gggtgtcgga gtcagtggcg
    23701 agggccagac ggacgcgccc gatcttgacc ggcgactcta actccacgtc ggctccggtg
    23761 cggaaacact cgtcggcgac gtgcgaacgc ttcaccgtca ggctgccggc ctcgccgccg
    23821 cccgagatga tgcgaaactt gcggccctct cggtagtact cgcccgcgaa cttcgccagc
    23881 ggatcatcgc ccgactcatc catcggcggg gagacgaact tcccgccgct gaccaagacg
    23941 accgggtcga tagtcgtccc catctcgccc gacacggcaa agatcatcgc ccccttcgcg
    24001 ccgccggcct gttgcgcgtg cgcgcccgag aagcacgcga gcagcagcgt cagcgccgcc
    24061 gcgagtcgcg ctctcgccgt caacgaatgc tgttgtttgc tcatcgtcaa ctatgtcccg
    24121 attgttccgc tcagatgttt gaacagtacc gcgagcggta gcgagcgggt gtagatgcgc
    24181 ggtgcgggcc tctcgtcgca gccgcactcg cttgctaccg ctcgcggtac tgttttcaga
    24241 tcgccgtgcg cttctccttg tcgctcgcgc cggggcggat tttctcggag acgactttgg
    24301 cttgcatgaa gaggccgagg tagtcgcgcc cgcccgcttt cgagtcggtg cccgacatgt
    24361 tgaagccgcc gaaggggtgg acgccgacga gcgcgcccgt gcatttgcga ttcaggtaga
    24421 ggttgccgac gtggaattcg cggcgcgcgc gctccagctt ctcctcgtcg ttcgtgtaga
    24481 cggcgcctgt gagtccgtac tcggtgtcgt tggctatcgc cagcgcgtcg tcgaaatcgc
    24541 gcgcgcgaat gcaggcgagg acggggccga aaatctcctg ctgctcgata gtgtcgccag
    24601 ctttcacgtc ggcgatgacg gtcggctcga tgaagaagcc ttgctcgccg tcggcgccgc
    24661 cgcccgcgac gacgcgcccg ccgtctgcct tgccgcgctc gatgtactcg ttgatggact
    24721 tgaacgcctt gttgttgatg acggcgctca ggctcgtccc cggtgcgttg gggtcgccca
    24781 gcttgagacg ctcggtgcgc tcgacgatct tctcaatcat cgagtcgtaa acttgcgtgt
    24841 ggatgatggc gcgcgagcag gccgagcact tctgaccctg aaagccgaag gccgactgca
    24901 caacgcccgt ggccgcctca tcgagatcga cgcccgcgtc aacgatgatg gcgtccttgc
    24961 cgcccatctc ggcgacgacg cgcttgatcc agatttgtcc cgcgttcacc ttcgccgcgc
    25021 gctcgttgat gcgcaggccg atctccttcg agccggtgaa agcaacgtag cgcgtcttcg
    25081 ggtgatcgac gatcacgtcg ccgacttccg cgcccgagcc tgtcgtgaag ttaacgacgc
    25141 cgggaggcac gccggcctct tcgagcagct cgaagaactt gtaggcgatg gtcggggcat
    25201 cggacgaggg cttcagaacg acggtgttgc cggtgacgat agaggcgacc gtcataccgg
    25261 ccatgatagc gagcgggaaa ttccagggcg ggatgacgac gccgacgccg agcgggatgt
    25321 attcgagccg gttctcctcg ccttcgatct tcacgagcgg ctgccctccg gcgtagcgca
    25381 gcatctcgcg cgcgtagaag tcgcagaagt cgatggcctc ggcggtgtcg ccgtcggcct
    25441 cggcccacgt cttcgccacc tcgtagacca tccacgccga catctggtga cggcgctcgc
    25501 gaagcagatc ggcgacgcgg aacagaagcg cggctcgctc ctcggccggc gtgcgactcc
    25561 acgtcttaaa ggtctcgctg gcgcgctcga cggccaggcg agccaactcc tccgtggcct
    25621 tctggaagcg tccgaccacc tgcgtcttgt tggcggggtt gaagctctcg aaggtgcgcc
    25681 ccgtcatgat gcgctcgccg ccgatgacga gcgggtagtc gcgccccagc tcgcccctca
    25741 cgcgctccag cgcctcgcgc atcgcgctgg cgttttcctc cccgctgaag tcggtgaacg
    25801 actcgttccg aaactcgttc tgcataagtt gctcctcaat aaatgatgaa gtcaggagtc
    25861 agaagtcaga agtcagaagt atgtggccgg gttacttgcc gcccacgtcg gaagattcaa
    25921 gccgggcggg ctagaacttc tgactcctga ctcctgactc ctgactactt ttttttcatg
    25981 ctaccacaac gcggacgcaa aaagcgcggc cggactttca agctggaagc gtccggccgc
    26041 gcgatttcgt tccgtgttct gcggttcgct cagacccact tgccggcctt cgccagcttc
    26101 ttgatcttgc gcttgaccag ctcgcgcttg agcggcgtgg tgtggtcgat gaagacgatg
    26161 ccgtcgcaat ggtcggtctc gtggagcatg caccgggcct caagctcaag gccgtcgtac
    26221 tcgaactcct cgccgttgat gtcctgcgcg cgaacgacgg cccgcaagct acgtttcacg
    26281 ttgaagtaga tgccggggaa cgacaggcag ccctcgtcgc ccgtctggtc gccttcgacg
    26341 cggagcacga cggggttgat gagcgcgacg cgcgcgccgg gatcgtcgcc gcccgagcag
    26401 tccatcacga acagacgttt cgagagtccg acctgcggcg cggccagccc gacgccgctc
    26461 gccgagtaca tcgtctcgaa catgtcgccg acgagctttt ttagcccgtc gtcgaactcg
    26521 gtgacggggt cgcccgactg ttcgagcacc gggtcgccat attttacgat cttcagaagt
    26581 gccatcttta attcccgaaa cttcggcgtt ttgcctgagt ttcgatgcta atcttacgcg
    26641 aaatgatgct tcgaggccaa cggcggagaa aggatgaggg atgagggatg agggatgagg
    26701 gatgaaggat gaaggggagt cagcagtaag cagtaagcgg taggcagtca aggcgctcgc
    26761 ccggccttga ctgcttactg cttactgctc actgcttact atcttctttc atccctcatc
    26821 cctccgccct catccctgga tgagtcgttc cacgtcgcgg aaggtcgggt cttcggcgta
    26881 gagcttctgg agttcgcggt tggcctgtgc ggcacggccg agcttgaggt aagagagggc
    26941 gcggcggtag acggcctcgc gcagcagggc ggggctgcgt cccttcttct tcctcagcgc
    27001 gccggtgaag acgctcacgg ccgcctcgtg gagtcccttc tcctgcatgg cgcggccgta
    27061 gaagatgacg atttcgagcg tcaggtcgtc cgagacttcg acgccctgcg cgcggtcgat
    27121 gatgccgtcc cacgcgcccg cgtccgcgta caactcgcag agcgagagcg tcagcgccgg
    27181 ctccgggtct aagtcggcga cttcttcgag cagccccgtg gcgtcgtcca ctcttccctg
    27241 ctcctgatag aactcgacga gcagcaacgc aacggccaac tcatcgagcg ccaccgaggc
    27301 gcgcacgctc ggcgtaatct ggacgggcgc ctcgacgccg ggcagatact tccgcatgag
    27361 cggcgtcggc aactccgcgt cgctttgcac gagccgctcc agcatctcga cggcgcgtgc
    27421 gcgcccctcc gtgtgtcgcg agaggatgga agccgcgtag acggccgcgc ccggctcttc
    27481 gtccgccgcg gagaggaaat gctggagggc cgtgtccgcg cggcccgcgc cgagttcgtt
    27541 caatccttcg acgaaatcct tctcgtgccc cggtgcgaag aagcccggct cgggcgtctc
    27601 ctgcgtgacg acctccggcg cggacggcgg tgcggcgggc agtgcatgcg cgacgcgcgt
    27661 ccgccgtccg cccccactac tgcccaaatg cttgacgtag ctcacgcccg cgcccggcag
    27721 atcgacactc agacgcgggc cgcgcggccc cgtccccacg cgcagcgggc cgacgccgac
    27781 gctcccgccg atgccgctcc ggctcaggtt gagtcggaaa aatttgccga gccggatgct
    27841 cttgctcaga cgaaagccca ctcgtcgcgc gctcctttca tttgttccaa aggctcaacg
    27901 ccagtcacgt ctgtcggggc agagcttgtc cgcggccccc gcttccagaa gaattaacat
    27961 ggatggacag gagttgcagg atataaagac agatttcaaa tttcagattt caaatcttcc
    28021 catcctgcgt atcctgtcca tccatgttaa ttccgccgta gcttcgggcc gcggacttgc
    28081 cctgccccta cagttttgaa agacactttc actcgtcgcc cttcgcggcg ctcgtcttgt
    28141 gcttgccaaa caccggctcg cgcttctgca tgaaagccgc gacgccttcg cggaagtcgg
    28201 cggagcggaa gcagtcggcc tgaccgtcgg cttcgtattc gagggcggcg gcgagttcct
    28261 cctgctcggc gcggctgagt gcgcccttga tgcgtgcgag cgagggctgc ggcgcggcgg
    28321 ccatccgcgc ggccaactcg ccgaccactg cctccaactc attcgcggcg acgacccggt
    28381 tgacgaggcc gagccgcaac gcctcctccg ctttgacggt cgcgccggtc gccatcagct
    28441 cgaaggcgcg ggtgcggccg acgagacgcg gcaaaaagaa agtgctgccg ccgtcgggca
    28501 tgaggccgac gcggacgaag ctctggctga aggtggccgt ctccgt
//
LOCUS       DASVES010000397        10126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1215266, whole genome shotgun sequence.
ACCESSION   DASVES010000397 DASVES010000000
VERSION     DASVES010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215266"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VF240_16340"
     CDS             complement(<1..733)
                     /locus_tag="VF240_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176832.1"
                     /translation="MKVSTLRAKSFVVLIALSLLTSCTQLPDLKPFADATTRMAGAMR
                     QGYAQTEGLLIRAELDSPRINELRARWDSTKKAINAIVAYSDALAALADAGNKGEESA
                     GALISSLEGVQTAVSALLPGIPGIPATVTTVFRKINGVVAKLRARKALKEAVNDADEA
                     LNATATILSLNFQELARLNRAAGQEILSKLEFDNNTLRDYHDGLTAEKNQITPVLTMI
                     LAYQNKDNESTRPITLRDLRNSDPGF"
     gene            complement(1119..2123)
                     /locus_tag="VF240_16345"
     CDS             complement(1119..2123)
                     /locus_tag="VF240_16345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7176833.1"
                     /translation="MTNYGAPLDLIIIGAGPAGISAADAAAREGLDYLVIERGLIADT
                     VYRYPVGLTVFSTPEELEMREGTLRPCREKPTREELLSHYVRFALEESLRINTEEEAV
                     EMERDADADGFVVRTTRSRYRARRVLVATGAMARPRRLNVPGEDLPKVHQRFVEPYPY
                     VRKDALVVGGGNSAAEAALFLAEGGARTTLAIWPADWENRDPKRGAIKHWVRAPLERE
                     IERGRLRLFPFSEVEEIGPRFVKIKDAGGKTLTLPNDAVFILVGSDPDLTLLRRLGVE
                     TKRAGLCDVPVYDPHTFETNVSGLHVAGHFTHARHIKEAIAVPRRIVPLIAQSLRASV
                     "
     gene            complement(2266..5967)
                     /locus_tag="VF240_16350"
     CDS             complement(2266..5967)
                     /locus_tag="VF240_16350"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7176834.1"
                     /translation="MNKHPLPLQLFQVLEEEYLSLHGDLTPKEEVTVNGRPGIVAMLD
                     YEFHAGHVKAPGRLVKKLLAKQRKDTGKGHLPSHLRGAAPKLRQALDDYADPGKRAAA
                     DLEGGLDALLKVHIYEPDAFKYVNLQDATRTLVENYADAPAGSDALAYVNRLLLEEAF
                     PDELEKVSDIRQAAMFKRLHGQKQSAICLSGGGIRSGTFALGLLQGLARFNLLKEFDY
                     LSTVSGGGYIGGWLTAWLYRHPRGLDGVTEDLGRAAPKSKIDPDPAAVRHLREYSNFI
                     TPKAGLMTADTWAFIAIYLRNLLLNWMVLFPLVLAVLALPRLNVSLLYSQADWAGRAP
                     AALAPLAAAFDLRHIFLGVGSLLLVLAIAYVSLSRPAVSAQLIERSKFWRPRRGQRSF
                     LKWCLLPLVGAAFCLTTYWAWSSEASQGDAVSQFSGLNIFGVEITKKMWYVLFGIAIP
                     VVSWLISSFFLERFQHPKEFTKLEVTVFILLVLVGAIGGWLMWWATLQEWSNPAVRDF
                     VEAYAAGWPAEWYACLAVPVALVVLLLLTTVFVGLTSRGFFGGAELLNDEDREWWARF
                     NAWVLIAAMAWAVFSILSIHGPRLFFAAPSWVASAGGISGLISLLAGYSAGTPAHDDQ
                     ASKGGLLKAFFHQHLLPLLALVFLLVFFIAMSLLVTGLLYAAWPLNGQSMWGGANFLA
                     MPFGRPTHMAVVHFARFWYVLVFILIAAGICTFLALRINLNKFSLHAGYRNRLIRAYL
                     GASRLKAERRENPFTGFDPEDNVPMHELRPVLFHEGDFEDLEGLAGKIARAEDPTSKF
                     LEEQLSAATRDELKDYDGATPLPQRLRINLITDLNQVLESEMFVVNTAGGADAPCLVK
                     SAARGGAQQSDHVILGKRHALRAVYPAEIKKKYPPPHRLLHVINTSLNLVGGKNLAWQ
                     QRKAEPFAITPLHSGCFRVGYRRSRDYGGHQGISLGTAAAVSGAAASSNMGYYTTSPV
                     ISLVLTLFNVRLGWWLGNPGPAGIKTYYREAPRLSLSPVLHEAFGLTDDTHKYVYLTD
                     GGHFENIALYEMVLRRCRVIVVSDAAQDGEFQFNDLGNAVRKIRIDLGVPIDFDEMPI
                     YKKQPGDKKASYWAFGKIGYSQADPGAEDGVLIYVKPAVYGENEPRDVLQYKQANDDF
                     PHQSTGDQFFDEPQLESYRMLGFYIMNRLCDNEFDTTTGVALPQRNLNIAEFIRAARQ
                     NYAQHTKGGEPVWLKELFVADKNYLVRDH"
     gene            complement(6183..6980)
                     /locus_tag="VF240_16355"
     CDS             complement(6183..6980)
                     /locus_tag="VF240_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176835.1"
                     /translation="MRGPCCEPFSVADACLTASALSFTKATIKQGCRGQYNCTAFPSE
                     PSHDGDAAASHCQEARAARPCRVDFNPGLFNFVTTAVGRREMFCPGQNKEVRIGPYLA
                     VGASTYLDKNELQGDQDVKVKDTNGGDNRAELDTERAKIDNDLNYYAEGGLIGNFFRF
                     KKNPDSDTVSQELFLQAIIGYGRYEALTGFNPGKSGFFNDSRNRFIGKLRIFPTFLDT
                     APDGGADSSPMFGVEINAGRGPDQIKFFTGVATAFKLFSGKKKTDTQ"
     gene            7286..8314
                     /locus_tag="VF240_16360"
     CDS             7286..8314
                     /locus_tag="VF240_16360"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7176836.1"
                     /translation="MSRHDQSSESAPRPAGLRAKLRGILIPFTTPFDAEGGVDVRAVR
                     ANVGRWIGTGVSGYVALGSTGERVHLDEDERLMVVEAAREAAPLESAYVVGVGAHSTR
                     GTIRETAAAARAGADAVLVITPHFYRASMTQEALLTHYRAVADASPVPLILYNIPQNT
                     GVALAPETVASLSEHANVAGIKDSSGDVVNFAEMLRQAGGRGSKREHQHGREFVSMTG
                     HAGVLYPALAAGAGGAVLAVACVVPELCVAVHQAFRAGDHARARALGERLLPVARAVT
                     TRYGIGGLKFALDLRGYAGGRVRAPLRMPDAEARSEIARLLEEAADFDDANSAEVVNR
                     QSSIVNRG"
     gene            8414..10099
                     /gene="bshC"
                     /locus_tag="VF240_16365"
     CDS             8414..10099
                     /gene="bshC"
                     /locus_tag="VF240_16365"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEX7176837.1"
                     /translation="MTTGEGNCQGTPQAAGLRVERLPFERIPQQSRLFLDYLRDPLTL
                     QKFYPTAVRFHYETPARAAEVLKAHEVERGALADALGEMNAAWGAGAETLASVERLRS
                     PQSVAVVSGQQVGLFTGPLYTIYKALSAVKLARCLTERGVEAVPVFWMATEDHDWPEV
                     QRAEVTGCDGRLESVGVPAELHAEGAPVGGVLLDASVEETIAGLLVALPRSEFTSDLE
                     QLVRDCYLRGRTYGEAFARMLTALTGKFGLVLLDPTDARLKRLAAPLYERAARRAPEI
                     AAALVERSRELESAGYHAQVHTTPDTFPLFIHDSEGARRAITRSAESRYQAKGGECEW
                     TLAELAEMTAREPERFSPNVTLRSVVQDYLLPTVAYFGGAAEVAYFAQTAEVYRLLAR
                     PVTPIFHRASMTLVERRVGRTLERYGLRMEDFFAGLDAVAARVVEEHLGRDVALAFGR
                     TDETIERALDDLGAEIAKFDPTLADALKGGRGKIAHHLEGLRTRFHRAQLERDNVVHR
                     QLERAANTFYPDKTLQERRVNVTSLLARHGRDALDWIFDTIDPDSADHQIVYL"
BASE COUNT         1664 a         3171 c         3593 g         1698 t
ORIGIN      
        1 cgaagccagg gtcggagttt cgcaggtcgc ggagggtgat cggccgggtg ctttcgttat
       61 ccttgttctg gtaggcgaga atcatcgtca ggacgggggt gatctggttc ttctccgccg
      121 tcaggccgtc gtgatagtcg cgcagggtgt tgttgtcgaa ctccagcttg ctgaggattt
      181 cctgtccggc ggcccggttg aggcgcgcga gttcctgaaa gttgagcgag aggatggtgg
      241 ccgtcgcgtt gagggcctcg tcggcatcgt tgaccgcctc cttgagcgcc ttgcgcgcgc
      301 gcagcttggc gacgacgccg ttaatcttgc ggaacaccgt ggtcacggtc gcggggatgc
      361 cggggatgcc gggcagtagc gccgagacgg ccgtctgcac gccttcgagc gacgagatca
      421 gcgcgccggc cgactcctcg cccttgttcc cggcgtcggc cagggccgcg agcgcgtcag
      481 agtaggcgac aatcgcgttg atggcttttt tggtcgagtc ccagcgggcg cgcagctcat
      541 tgattctcgg gctgtcgagt tcggcgcgga tgagcaggcc ctcggtctgc gcgtagccct
      601 gccgcatcgc gccggccatc cgggtggtgg cgtcggcgaa cggcttgagg tcgggcagtt
      661 gggtgcacga cgtgaggagc gacagggcga taaggacgac gaagctcttg gcccgaagcg
      721 tggagacttt cattgtgttt cttacctttc gagaaggggc cgcgcgccgc ggggaacgtg
      781 gctggcccgt cggaaacgtc cgcgagagaa gacgccgtga aaagatggac gcgcggggcg
      841 ggcgaaagat gacgccgcgc ggccgagggc gccggtaaca aacgtcgccg ggcagcgggc
      901 caactcacaa gcccgtccgt cggctcaagt ccagacgcct tgcaacggca actctacacg
      961 ttatgcgggc cggggtcgaa acatttcacg ggatgcccgc gcgggagata aaaaagaaga
     1021 ggccgggtcg gaagcgatct gccgcgagga ggacgggcgg ggcggcgacg caggtcgcgg
     1081 cgttggtcgt gaggggggac gcttcagtgc ccttcggctc agactgaggc gcgtaaggat
     1141 tgggcgatga gggggacgat gcggcggggg acggcgatgg cttctttgat gtggcgggcg
     1201 tgggtgaagt ggccggcgac gtggaggccc gagacgttgg tctcgaaggt gtgggggtcg
     1261 tagacgggaa cgtcgcagag gccggcgcgc ttcgtctcga cgccgaggcg gcgcaggagc
     1321 gtgaggtcgg ggtcgctgcc gacgaggatg aagacggcgt cgttggggag tgtcagggtc
     1381 ttgccgccgg cgtcttttat cttgacgaag cgcgggccta tctcttcgac ctcggagaag
     1441 ggaaacaggc gcaggcgccc gcgctcgatc tcgcgttcga gcggggcgcg cacccagtgc
     1501 ttgatggcgc cgcgtttggg gtcgcggttc tcccagtcgg cgggccagat ggccagagtg
     1561 gtgcgcgcgc cgccctcggc gagaaagagc gcggcctcgg ccgcggagtt gccgccgccg
     1621 acgacgagcg cgtctttgcg cacgtagggg tacggctcga cgaaacgctg gtggactttg
     1681 ggcagatctt cgccgggaac attcaggcgg cggggacggg ccatcgcgcc cgtggcgacg
     1741 agcacgcgcc gcgcgcggta acgcgagcga gtcgtgcgga cgacgaagcc gtccgcgtcc
     1801 gcgtcgcgct ccatctcgac ggcctcctcc tcggtgttga tccgcaggct ctcttcgagc
     1861 gcgaagcgga cgtagtgcga gagcagttcc tcgcgcgtcg gcttctcgcg gcaggggcgg
     1921 agtgtgccct cgcgcatctc caactcctcg ggcgtggaga agaccgtgag gcccacgggg
     1981 tagcggtaga cggtgtcggc gatgaggccg cgctcgatga cgaggtagtc aagcccctcg
     2041 cgcgccgccg cgtcggccgc ggagattccc gccggccccg cgccgatgat gatgaggtcg
     2101 agtggcgctc cgtagttcgt catgcgtctg gccgcaatcc tcgcatgtcc cgctccagtc
     2161 atcaactgtc ggcgccaccc atcggccgcc cccgcggaca aaaaattgcc cggcgctgct
     2221 cacaaacggg gcggcgccgg gcagttctga ttggcttgcg gcggcttagt gatctctcac
     2281 cagataattc ttatcggcta cgaagagttc tttgagccat acgggttcac cgcctttggt
     2341 gtgttgcgcg tagttttggc gggcggcgcg gatgaactcc gcgatgttca gattgcgctg
     2401 cggcagcgcg acgccggtcg tcgtgtcgaa ctcgttgtcg cagaggcggt tcataatgta
     2461 gaagccgagc atccggtagc tttcgagttg cggctcgtcg aagaactggt cgccggtgga
     2521 ctggtgcggg aagtcgtcat tggcctgctt gtactggagc acgtcgcgcg gctcattctc
     2581 gccgtagacg gccggcttga cgtagatgag aacgccgtcc tcggcgccgg ggtcggcttg
     2641 gctgtagccg atcttgccga aggcccagta ggacgctttc ttgtcgcccg gctgcttttt
     2701 gtagatgggc atctcgtcga agtcgatggg gacgccgagg tcgatcctaa tcttgcggac
     2761 ggcgttgccg aggtcgttga attggaactc gccgtcctgc gcggcgtcgc tgacgacgat
     2821 gacgcggcag cggcggagca ccatctcgta gagggcgatg ttctcgaagt ggccgccgtc
     2881 ggtgaggtag acgtacttgt gcgtgtcgtc ggtcaggccg aacgcctcgt gcaggacggg
     2941 cgagagcgag aggcgcgggg cctcccggta gtaggtcttg atgccggccg ggccggggtt
     3001 gccgagccac cacccgaggc gcacgttgaa gagcgtcagg acgagcgaga tgacgggcga
     3061 ggtggtgtag tagcccatgt tggagctggc ggccgcgccc gagacggcgg cggccgtgcc
     3121 cagcgagatg ccctggtgac cgccgtagtc gcgcgaccgg cggtagccga cgcggaagca
     3181 gccagagtgc agcggggtga tggcgaaagg ctcggccttg cgctgctgcc acgccaggtt
     3241 cttgccgccg acgaggttga gcgaggtgtt gataacgtgc aggaggcggt gcggcggggg
     3301 gtacttcttc ttgatctcgg ccgggtacac ggcccggagc gcgtggcgct tgccgaggat
     3361 gacgtggtcg ctctgctgcg cgccgccccg cgcggcgctc ttgacgaggc acggggcgtc
     3421 ggcgccgccc gcggtgttga cgacgaacat ctcgctctcg agcacctggt tgaggtcggt
     3481 gatgaggttg atgcgcagac gctgcgggag gggggtcgcg ccgtcgtaat ccttcagctc
     3541 gtcgcgcgtg gcggcggaga gctgctcctc gaggaacttc gaggtcgggt cttcggccct
     3601 cgcgatcttg ccggccagcc cttcgaggtc ctcgaagtcg ccctcgtgga agaggacggg
     3661 gcgcagctcg tgcatcggga cgttgtcctc ggggtcgaag ccggtgaagg ggttctcgcg
     3721 ccgctccgcc ttgaggcgcg aggcgccgag gtaggcgcgg atcaggcggt tgcgataccc
     3781 ggcgtggagg gaaaacttgt tgaggttgat gcgcagcgcg aggaacgtgc agatgcccgc
     3841 cgcgatgagg atgaacacca ggacgtacca gaaccgcgcg aagtggacga ccgccatgtg
     3901 cgtggggcgg ccgaaaggca tggcgaggaa gttcgccccg ccccacatcg actgcccgtt
     3961 caacggccag gccgcgtaga gcaggccggt gacgaggagc gacatcgcga tgaagaagac
     4021 cagcaggaac accagcgcca ggagcggcag caggtgctgg tggaagaatg ccttgagcag
     4081 cccgcccttg ctcgcctgat cgtcgtgcgc gggcgtgccg gcgctgtacc cggcgagcag
     4141 cgagatgagg ccggagatgc cgccggccga ggccacccac gacggcgcgg cgaagaagag
     4201 gcgcgggccg tgaatcgaga ggatgctgaa gaccgcccag gccatggccg cgatcaggac
     4261 ccacgcgttg aagcgcgccc accactcgcg gtcctcgtcg ttaagcaact cggccccgcc
     4321 gaagaagccg cggctcgtga ggccgacgaa gacggtcgtc aaaagcagca gcaccacgag
     4381 cgcgacgggg acggcgaggc aggcgtacca ctcggcgggc caccccgcgg catacgcctc
     4441 gacgaagtcg cgcaccgccg ggttcgacca ctcctgcagc gtggcccacc acatcagcca
     4501 cccgccgatg gcgccgacga ggacgagcag gatgaacacc gtgacctcca gcttggtgaa
     4561 ctccttcggg tgctggaacc tttcgaggaa gaaggagctg atgagccagg agacgacggg
     4621 gatggcgatg ccgaacagca cgtaccacat cttcttcgtg atctccacgc cgaagatgtt
     4681 gaggcccgag aactgcgaga cggcgtcgcc ctgactcgcc tcgctcgacc acgcccagta
     4741 agtcgtcagg cagaaggccg cgcccaccag cggcagcaga caccacttga gaaaactcct
     4801 ctgcccgcgc cgcggccgcc agaatttgct gcgctcgatg agctgcgcgc tgacggccgg
     4861 gcggctgagg ctgacgtagg cgatggccag caccagaagg agggagccga cgccgaggaa
     4921 gatgtggcgc aggtcgaagg ccgcggccag cggcgccagg gccgccgggg cgcgcccggc
     4981 ccagtcggcc tgcgagtaga gtagcgagac gttgaggcgc ggcagcgcca gcaccgccag
     5041 cactagcggg aagagcacca tccagttgag cagcaggttg cgcagataga tggcgatgaa
     5101 ggcccaggtg tcggccgtca tcagcccggc cttcggcgtg atgaaattgc tgtactcgcg
     5161 caggtgccgg acggccgccg ggtcggggtc gatcttcgac ttcggggcgg cgcggccgag
     5221 gtcttcagtg acgccgtcga ggccgcgcgg gtgcctgtac agccaggccg tcagccagcc
     5281 gccgatgtag ccgccgcccg agacggtcga gaggtagtcg aactccttga gcaggttgaa
     5341 gcgcgccagc ccctggagca gcccgagggc gaaggtgccg ctgcggatgc cgccgcccga
     5401 gaggcagatg gccgactgct tctgcccgtg gaggcgcttg aacatcgccg cctggcggat
     5461 gtcggagacc ttctccaact cgtcggggaa cgcctcctcc agcaggagcc ggttgacgta
     5521 ggccagcgcg tcggacccgg cgggcgcgtc cgcgtaattc tcgacgaggg tgcgcgtcgc
     5581 gtcttgaagg ttgacgtact tgaaggcgtc cggttcgtag atgtgcacct tcagcagtgc
     5641 gtcgagcccc ccttccaggt cggccgccgc ccgcttcccg gggtcggcgt agtcgtcgag
     5701 cgcctgcctg agcttgggcg cggcgccgcg caagtggctg ggcaagtgcc ccttgcccgt
     5761 gtctttgcgc tgcttcgcga gcagcttttt gacgagccgc cccggcgcct tgacgtgccc
     5821 cgcgtggaac tcgtagtcga gcatcgccac gatgccgggg cgcccgttga cggtaacctc
     5881 ctccttaggc gtgaggtcgc cgtggaggct gaggtactct tcttccagca cctgaaagag
     5941 ttggagcggg aggggatgtt tgttcatgtt cggcacctga gaggggcttt ggggccagga
     6001 gggaatggcc gacgggcctg catcgcgccg gccggccgct caaacataca ggcctttttc
     6061 gcttatacat cccgcgtcgg ccgacgagtc acggccagca cgggaggtcg atggcaacaa
     6121 gaaggttcta caacttcagg cgtcaccggc ggctggcgac cgcctgcggt gtgtctgagc
     6181 atttattgtg tgtcagtctt tttcttaccg ctgaacagct taaacgcagt agcgaccccc
     6241 gtgaagaact tgatctggtc tggccctcgt ccggcgttga tctctacgcc gaacatgggc
     6301 gaggagtcgg cgcctccatc tggggccgtg tctaagaagg ttgggaagat tctcagcttc
     6361 ccgataaatc tgtttcggga atcgttaaag aaaccagact tgccgggatt gaacccggtc
     6421 agtgcttcat agcgtccgta gccgatgatg gcctgcagga acaactcctg agacaccgtg
     6481 tcgctgtccg gattcttctt gaagcggaag aagttgccga tgagaccgcc ctcggcgtag
     6541 tagttgagat cgttgtcgat tttcgcgcgc tcggtatcga gttcggcccg attgtctccc
     6601 ccattggtgt ccttcacctt aacgtcctgg tctccctgta actcattctt atcgagatag
     6661 gtggacgcgc ccacggcgag gtaaggcccg attctgactt ccttgttctg gccggggcaa
     6721 aacatttcac ggcggcccac cgcggtggtg acaaagttga agaggcccgg gttgaaatcg
     6781 acccgacacg gacgcgccgc gcgcgcctcc tgacaatggg aagcggcggc gtcgccgtcg
     6841 tgcgatggct cagacggaaa cgcagtgcag ttatactgcc cgcggcaacc ctgtttgatc
     6901 gtagcttttg tgaaagatag agccgacgcg gtaaggcatg cgtcagcgac cgagaagggt
     6961 tcacaacagg ggccgcgcat cgggattgcg cgccatcagt gaccaacggc cgtcgagcaa
     7021 cggcggcacg ataatactgt cccggtgcaa tgtcaataac attatgcaaa gtgtttgctg
     7081 ttggggttat ggcccgtcgc gacccgtggc aaagggccgt ccaaaggccg cccatgccga
     7141 cgaccacggc cgcggcgggc actcgactct gacggtctaa acgtcgggag tgggggccgt
     7201 cagtggcggc ttcaacgcgg cacaggtcag gcggcgacat catctggttt gcgtgccttc
     7261 agcgcgcctc gttataatcc ccggcttgag ccgacacgac caatcatccg aaagcgcccc
     7321 gcgcccggcc gggctgcggg caaagctccg cggcatactc attccattca cgaccccctt
     7381 cgacgccgag ggcggcgtgg acgtgcgcgc cgtgcgcgca aacgtcgggc gctggatagg
     7441 gacgggcgtg tcgggctacg tggcgctcgg ctcgacgggc gagcgcgtgc atctggacga
     7501 agacgagcgg ttgatggtcg tcgaggcggc gcgcgaggcg gcgccgttgg agtcggccta
     7561 cgtcgtgggc gtgggcgctc actcgacgcg agggacgatc cgcgagactg ccgccgcggc
     7621 gcgcgccggg gccgacgccg tgctcgtcat cacgccccac ttctaccgcg cgtcgatgac
     7681 gcaagaggcg ctcctcacac actatcgcgc ggtggccgac gcttcgcccg tgccgctcat
     7741 cctctacaac atcccacaga acactggtgt cgcgctcgcg cccgagacgg tggcgagcct
     7801 ctcggagcac gcaaacgtcg ccggcatcaa ggacagctcg ggcgacgtgg tgaacttcgc
     7861 ggagatgctc agacaggcgg gcgggcgcgg gagtaagcgg gagcatcagc acgggcgcga
     7921 gttcgtgtcg atgacggggc acgcgggcgt gctctatccg gcgctcgcgg cgggtgcggg
     7981 cggggcggtg ctggccgtcg cctgcgtcgt gccggagctg tgcgtcgccg tgcatcaagc
     8041 cttccgcgcc ggcgaccacg ctcgcgcgcg cgctctcggc gagaggctcc tgccggttgc
     8101 gcgcgccgtg acgacgcgct acggtatcgg cggactgaaa ttcgcgctcg acctgcgggg
     8161 ctacgccggc ggccgagtgc gcgcgccgct ccggatgccg gacgccgaag cgcgctccga
     8221 gattgcgcgg ctgctcgaag aggccgctga cttcgacgat gcgaacagtg ctgaggtcgt
     8281 caatcgtcaa tcgtcaatcg tgaatcgcgg ttgagccggt atcgcgttga gcgtggcagc
     8341 tcaagttgag ttggagcaga ctccgaacga ttgacgattc acgattgacg attcacggct
     8401 tggcgggaga gttatgacga cgggcgaagg aaactgtcag ggcacgccgc aggcggcggg
     8461 gctgcgcgtc gagcgcctgc ccttcgagcg tatcccgcaa cagtcgcgtc tgttcctcga
     8521 ctacctccgc gacccgctca cgctacagaa gttctacccc acggctgtgc gcttccacta
     8581 cgagacgccc gctcgcgccg ctgaagttct caaggcgcac gaggtcgaac gcggcgcgct
     8641 cgccgacgcg ctcggcgaga tgaacgcggc gtggggcgcg ggcgcggaga cgctcgcaag
     8701 cgtcgagcgt ctgcgctcgc cccaatcggt cgcggtcgtc tccggccagc aggtcggcct
     8761 cttcacgggg ccgctctaca ccatctacaa ggctctgtcg gccgtgaagc tggcgcgttg
     8821 tctgaccgag cgcggcgtcg aggccgtgcc cgtcttctgg atggcgaccg aggatcacga
     8881 ctggcccgag gtgcagcggg cagaggtcac gggctgcgac ggccggcttg agtcggtcgg
     8941 cgtccccgcg gagttgcacg cggagggcgc gcccgtcggc ggcgtcctgc tcgacgcctc
     9001 cgtcgaagag accatcgccg ggctgctcgt cgccctgccg cgctccgagt tcacgtccga
     9061 cctcgaacaa ctcgtgcgcg actgctacct acggggccgc acttacggcg aagccttcgc
     9121 gcgcatgttg acggcgctca ccggcaagtt cggcctcgtg ttgctcgacc cgacggacgc
     9181 gcgcctcaag cgtctggccg cgccgctcta cgaaagggcc gcgcgccgcg cacccgagat
     9241 cgccgcggcc ttagtcgagc gcagccgaga gcttgagtcg gccggctacc acgcgcaggt
     9301 acacaccacg cccgacacct tcccgctctt cattcacgac tcggagggcg cgcgccgcgc
     9361 catcacgcgc agcgcggaga gtcgctatca ggcgaaaggc ggtgagtgcg agtggacgct
     9421 tgctgagctg gccgagatga ctgcgcgcga acccgaacgc ttcagcccga acgtcacgct
     9481 ccgctccgtc gtgcaggact acctgctccc caccgtggcc tacttcggcg gcgcggccga
     9541 ggtcgcctac ttcgcgcaga cggccgaagt ctaccgcctg ctcgcgcgcc ccgtcacacc
     9601 catcttccac cgcgccagca tgaccctcgt cgagcggcgc gtgggccgca cgctcgaacg
     9661 ctacggcctg cggatggaag acttcttcgc gggactcgac gccgtggccg cgcgcgtcgt
     9721 cgaagaacac ctcggccgcg acgtggcgct ggctttcggc cggacggacg agacaatcga
     9781 gcgcgctctc gacgacctcg gcgccgagat cgccaagttc gacccgacgc tcgccgacgc
     9841 gctcaagggc ggccgcggca aaatcgcgca ccacctcgaa ggcttgcgga cgcgcttcca
     9901 ccgcgcccaa ctggagcgcg acaatgtcgt ccaccgccag cttgagcgcg cggccaacac
     9961 cttctacccc gacaagacgc ttcaggagcg ccgcgtcaac gtcacctcgc tcctcgcgcg
    10021 ccacggccgc gacgccctcg actggatttt cgacaccatc gaccccgact cggccgatca
    10081 tcagatagtt tacttatgag tgataagtga tgagtgataa gtgata
//
LOCUS       DASVES010000398        13144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_845268, whole genome shotgun sequence.
ACCESSION   DASVES010000398 DASVES010000000
VERSION     DASVES010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13144
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845268"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF240_16370"
     CDS             <1..366
                     /locus_tag="VF240_16370"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEX7176838.1"
                     /translation="TVAIVSAGGVHLREQEPFNIADELGDLTFRVIPGDADSSQLTVT
                     HHHYDHADADRDINVVFPLDVLRDLRDEGFIGEVARKHVGYMGYTMQLKAMYEGTAPE
                     IANEIDRGSRADLVVLTGG"
     gene            415..669
                     /locus_tag="VF240_16375"
     CDS             415..669
                     /locus_tag="VF240_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176839.1"
                     /translation="MRGIPTVLITVSPEVSAQMRPPRALYPKGFKVGNSLGRPGMREL
                     QRQVLRDALQLLTENTRPGSFVTREYAEYGEQYEPPRVRK"
     gene            936..1952
                     /locus_tag="VF240_16380"
     CDS             936..1952
                     /locus_tag="VF240_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HEX7176840.1"
                     /translation="MTITQDQLRRIMPGANSARLALFLPHLQAAMDEFQINTPLRAAA
                     FLAQLAHESGQLRFMEEIWGPTSAQRRYEPVTSLSRTLGNTVTGDGFRFKGRGPIQLT
                     GRANYQRFGGLLGLDLVGNPSLAATPEVAFRIAALFWQNRGLNAFADTGNFREITRRI
                     NGGFNGLADRVNFYNRAKAILGVGSAAGPAGASRAGASRPSGRAGASPGRAGASPGRA
                     GAGAEDGGLSRGIMPGDPKHARMLSEGGAKAGAKAGAKKAGAKKAGARKAAGKKAGAK
                     KAAAKKGGAKKAVAKKAVAKKAGTKKGAAGKGGAGATKGAAKKTGARKGAASAKKSGA
                     KKRR"
     gene            2044..3162
                     /locus_tag="VF240_16385"
     CDS             2044..3162
                     /locus_tag="VF240_16385"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX7176841.1"
                     /translation="MLRTIGLVTGGGDAPGLNAVIRAVVKTAAGEYGLRVIGIEDSFE
                     GLLDETRTRELRPEDVRGLLPRGGTVLGTRNRGRFVERGAEGGHSHPDEIFKQAIANL
                     ERLGVDALVAVGGEGTLAIASEFERRGFHVVGVPKTIDNDLAATELTFGFVTALDIAT
                     EALDRLHTTAESHDRVMVLEVMGRHAGWIALHSGVSGGADVILIPEIPFSMERVAGKV
                     LERERCGSQFSIIVAAEGACEIAGKEVYQDAGSQQHSPRLGGIGHHVAAELGERTGKE
                     ARCVVLGHLQRGGSPNAFDRMLATNFGSAAVRALVRGERGVMVALQAANVITVPISHA
                     IANLKTVSPDSQLVRTARDIGVSFGATDEARHHKYEVC"
     gene            complement(3299..4354)
                     /locus_tag="VF240_16390"
     CDS             complement(3299..4354)
                     /locus_tag="VF240_16390"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7176842.1"
                     /translation="MIRTPSEKRKLVLFIDDDPSLLRLGRLTLERAGYQFLGALDGRE
                     GLRYARASRPDIILLDYMMPDISGKEVFRELVASTDAQLRRTPVIMLTARTNNDAEQR
                     ELMEMGLAAYLHKPFGYRELLNIIDNVLVLSQIKERNRVLELEARRAFVATVKTLIGL
                     LFAKDSYTGQHSQSVARLAEGLALQCGLSEEEAMYVKLGALLHDVGKIVVPETVLCKP
                     GALTPDEEDVMRRHVYHGEQALSGVPHMEQVRSMISHHHEWWDGTGYPAGLAGEEIIL
                     GARIISVVDAYDAMTSDRPYRARLPLAVAVERLRHGVGTQFDPVLVGTLFDYLSTHEQ
                     HEPQELDLQFLEELCVV"
     gene            4694..5188
                     /gene="greA"
                     /locus_tag="VF240_16395"
     CDS             4694..5188
                     /gene="greA"
                     /locus_tag="VF240_16395"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7176843.1"
                     /translation="MTEVIKQKLRDELETLESELRVHLPREIKRALEFGDLRENSEYK
                     AALERQSFVRARIGQLRQRISEISSIDISNIPRDRAAYGSTLVLYDPEREEELTYRLV
                     TSEESDPAAGLISTTSPIGRSLMGREEGDEVKVVTPAGAREFEIRSLRTLHDEAAEEK
                     KDEG"
     gene            complement(5322..6674)
                     /locus_tag="VF240_16400"
     CDS             complement(5322..6674)
                     /locus_tag="VF240_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7176844.1"
                     /translation="MLRRKTDLLERLRVPKPCSADWDSMSGNDRVRFCEHCRLSVNDL
                     SRMTRKEATRLVANSGGRLCVRYVQLPGGGVLTADAAPALYHIARRASRLAAGAAAAA
                     ISFGASVAAQTQSPSGRQSASGVEINVAARAAGRDALEPSGATLAGAVTDPLQARVAG
                     TSVTLSNTQTGETRTASSNDEGEYAFSNVPPGTYTLRFAAAGFRDYEAANVSVQGGGQ
                     GRVDATLQIGEITGGVMVMVAEPSEPLVKAAFKDDLAEVEKLLARGADANVLDRGIDS
                     TALAQAVAHANLKMVKALLDAGADINAEDRAGRTALMTLDEDATPDLVRALLDAGAEV
                     KVRDDDGNSALAHAAARSTAEVVRRLLDAGAKINMRNDEGRTALMLAAEVGELENARA
                     LVAAGADYNLKDDEGKTALDFARGGEYEEVTALLVAHGAVEGDKPKTEAEEEEEDEEP
                     "
     gene            7049..7891
                     /locus_tag="VF240_16405"
     CDS             7049..7891
                     /locus_tag="VF240_16405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7176845.1"
                     /translation="MFGASIGRGAQRIIGAMVRGLSRSSINPNVLTVIGVSINVFSGV
                     LFGLGHFFWAGVVLIVANLFDMLDGQVARLTGRVTRFGGFLDSSLDRLSDMAAFVGLM
                     VFYARYTDDPASLAYGQHSPLNVFLAGAALAGSVLVSYASARAESLIPKCDVGFLRRP
                     ERVVLLIIGGLSTHPGWSSPFANRMPAVLWVLTVGSYWTFAYRMYHTWSELQRTEGAT
                     TSNAAASDTTPRETVGEQTATAQTAAGPDTPQASAGAGGASNAGAGERHGTADVVRRP
                     QASG"
     gene            7901..8869
                     /locus_tag="VF240_16410"
     CDS             7901..8869
                     /locus_tag="VF240_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176846.1"
                     /translation="MSDNRRSSAKVSDNRCSFAGRERKRAALCPCCGAGVEGDMEAGC
                     AGCGARRVGPPLPRPERELPAYGHAFFSAALGLLLASAFVVATALALFERDAFALGLG
                     ALLAAAERAAWRLKWTLLPAAFIAAWACARLNRHMRRAPSRFVGHGFARAGLALSCAS
                     ALALALFVVASVPERLRRRELGRRAGEEATLYATDRVLFAYRARFGTYPSAPADLRRL
                     PDPDGSVAATVALLQSGSYAPEAEVASLAPKGRGRRRAAAPRLRSVSARGRDDSAGAG
                     LSLTSYELTLPGRDKVLGTADDLRLRDGVPLKAGDPPRRLATPTPV"
     gene            8899..9189
                     /locus_tag="VF240_16415"
     CDS             8899..9189
                     /locus_tag="VF240_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176847.1"
                     /translation="MGIFDIFRRRKSDDEASRRAQLLRTGRIAEGSIFDVVTDEAGHV
                     TQIFFNYTINGVDYESSQLLDPGQLLRQGDYFPGARVTIRFHPQRPGNSVVV"
     gene            9278..10831
                     /gene="accC"
                     /locus_tag="VF240_16420"
     CDS             9278..10831
                     /gene="accC"
                     /locus_tag="VF240_16420"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7176848.1"
                     /translation="MFRKILIANRGEIAVRVMRACREMDISTVAVYSEADAAALHVRL
                     ADEAYLLGPASSAESYLRIDRVIDAALRSGAEAVHPGYGFLAENAQFARAVTEAGLTF
                     IGPPAEVMELMGSKTSARRAATEAGAPVVPGTTEALASLAELKETARGLGYPVMLKAA
                     AGGGGKGMRLVETEAELASSFEAARSEAASSFGDDAVYLEKAVERPRHIEIQIFSDAH
                     GNHLHLGERECSIQRRHQKVIEEAPSPIDDPVLRASMGEAAVRVARACAYVGAGTVEF
                     LVSDVTREFYFLEMNTRLQVEHPVTELVTGFDLVREQIRVAAGEPLSFRQEDVVLRGH
                     AVECRVYAEDPENNFLPSPGRITHLRVPAGPNVRDDGGVYAGAEVSIHYDPMISKLAT
                     WGRTRAEAIAAMRRALDEYTVAGIKTTIPFFRQVMRDPEFLDGRLDTGFIARFEERRR
                     AALDAEENAGEAKDEEVVRRDMAAVAAALDYERRQRQQVSSADEGRARESSAWKMSGR
                     AALLQSRVR"
     gene            complement(10846..11445)
                     /locus_tag="VF240_16425"
     CDS             complement(10846..11445)
                     /locus_tag="VF240_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176849.1"
                     /translation="MTQNVLRQGRGLTRLGLLFVVPALAALMLASPAGRSSAVAPVSE
                     PAEPAFQVLPKPWTAVGSTGVIDEDSLNFFAFSNTDLGFRAGANALKIVARYNVTNTF
                     DNNANPNRPGWTRLEMGSTTPNSTIIEASLFQIRSCDQQQVLICTARNRSGDTPCARC
                     TFANSAVDFTNNLYYVEVTLSRGSAQTPSPRLHTLRLRQ"
     gene            11697..12224
                     /locus_tag="VF240_16430"
     CDS             11697..12224
                     /locus_tag="VF240_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7176850.1"
                     /translation="MKLIADIDGERHTLEVGREGGGRVVAEVDGRRHELEASEVEPGV
                     YLFVREGEVFEIHLGGAAGAAGASPPEISVRHRGGHGAYAVRVIDPKRLRGARAAAGH
                     DAGRAQITAPMPGKVVRVLVELGQEVEAGQPVVVVEAMKMQNELKSPKAGAVVALHAA
                     VGATVNSGDILAEIE"
     gene            12301..13068
                     /locus_tag="VF240_16435"
     CDS             12301..13068
                     /locus_tag="VF240_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX7176851.1"
                     /translation="MNRQLDASSLIPPPSSLADLRVLLWDIDGTLIRSRRTGAFKEYT
                     IPVLESVFGTAGRLRELSVSGMTDLQIVAESLRDEGFTPREIRARLRELSALYLAALE
                     RAAGAREGPPLFEVLPGVREVLTAAQAHPRYLSALLTGNIEPAARLKMRLVGLADFFT
                     LPGAFGDDSHDRRDLPALASERINRHLGLALLPSQFIVIGDTPNDIACARHFGARAIG
                     VGTGRVNSVETLRPHSPDALLPDLSDTNRVMEVLARL"
BASE COUNT         2069 a         4455 c         4465 g         2155 t
ORIGIN      
        1 accgtcgcca tcgtcagtgc gggcggcgtg catctgcgtg agcaggagcc gttcaacatc
       61 gcggacgagt tgggcgacct gaccttccgc gtcatccccg gcgacgcgga ctcttcgcag
      121 ttgacggtca cgcaccacca ctacgaccac gcggacgccg accgggacat caacgtcgtc
      181 ttcccgctcg acgtgctgcg cgacctgcgc gacgagggat tcatcggcga ggtcgcccgc
      241 aagcacgtcg gctacatggg ctacacgatg cagctcaagg cgatgtacga gggtacggcc
      301 cccgaaatcg ccaacgagat cgaccgcggc tcgcgcgccg acctcgtcgt cttgacgggc
      361 ggttgaccga acgtctgtca ccgcacggtc gttgcggtcc agcgcgagat cgagatgcgc
      421 ggcatcccca cggtgctcat caccgtcagc cccgaggtct cggcgcagat gcgcccgccg
      481 cgcgccctct accccaaagg cttcaaggtg ggcaacagtc tgggccgccc cggcatgcgc
      541 gagctacagc ggcaggtgct gcgcgacgcg ctccaattgc tgacggagaa tacgcggccc
      601 ggctcgttcg tcacgcgcga gtacgccgag tacggcgagc agtacgagcc gccgcgcgtc
      661 aggaagtgag cggaggcacg ccggcggcgc gtgcggggcg cgagccgccg gcgtgcgtct
      721 ttcgcgccga aagtttttaa ttgtctgccg ccgactctct gctacaatcg ccgccccgtc
      781 cgctggtagc ttttccattc catctcacct ccgaacgggc tttgaacgag cgggccgaac
      841 gactcgtcga ggcggcgctt ccgacgccag cgcgcgagtc agtctcgacc gtagaatact
      901 tacgcgaaaa tccccttgtc tgaggagaac aacaaatgac gattacccaa gaccaactgc
      961 ggcgcatcat gcccggcgcc aactcggccc ggctcgcgct tttcctccct caccttcagg
     1021 ccgcgatgga cgagttccag atcaacacgc cgctgcgcgc ggcggccttt ctggcgcagc
     1081 tcgcgcacga gtcgggccag ctccgcttca tggaagagat ttgggggccg acctcggcgc
     1141 agcgccgcta cgagccggtg acgagtctgt cgcgcaccct cggcaacacg gtgaccggcg
     1201 acggcttccg cttcaagggc cgcggcccga tccagctcac ggggcgcgcc aactaccagc
     1261 gcttcggcgg cctgctcggc ctcgacctcg tgggcaaccc gtcgctcgcc gccacccccg
     1321 aggtcgcctt ccgcatcgcc gccctcttct ggcagaaccg cggcctcaac gccttcgccg
     1381 acaccggcaa cttccgcgag atcacccgcc gcatcaacgg cggcttcaac ggcctcgccg
     1441 accgcgtcaa cttctacaac cgcgccaagg cgattctggg cgtcggctcc gccgccggcc
     1501 ccgcgggtgc atctcgggcc ggcgcgtcga ggccgtcggg ccgcgcgggc gcatcaccgg
     1561 gccgcgcagg tgcgtcaccg ggccgcgcgg gtgcaggggc cgaggacggc ggactctccc
     1621 gcggcatcat gcccggcgac ccgaaacacg cgcgcatgct gagcgagggc ggcgcgaagg
     1681 ccggcgcaaa ggccggtgcg aaaaaggcgg gcgcgaagaa agctggcgcc aggaaagccg
     1741 ccgggaagaa ggcgggtgcc aagaaggccg cggctaagaa gggcggcgca aagaaagccg
     1801 tcgcgaagaa ggctgtcgcc aagaaagccg gcacgaagaa gggcgcggcc ggcaagggcg
     1861 gcgcgggcgc cacaaagggc gcggcgaaga agaccggggc caggaagggc gccgcgtcgg
     1921 cgaagaagtc gggcgcgaag aagcgtcgct gacgacagta ccgtgagcga tagcgagcgg
     1981 gtcgggtcac gcgaagcccg aagcgctcgc tatcgctcgt agtaatctct cgctttctca
     2041 actatgcttc ggacaatcgg actggtgacc ggcggcggcg acgcgccggg gctgaatgcg
     2101 gtgatccgcg cggtggtcaa gaccgccgcg ggcgaatacg gcctgcgcgt catcggcatc
     2161 gaggacagct tcgagggact gctcgacgag acgcggacgc gcgagctgcg ccccgaggac
     2221 gtgcgcgggc tgctcccgcg cggcggcacg gtgctcggca cgcgcaaccg cgggcgcttc
     2281 gtcgagcgcg gcgcggaggg cggacactct catccggacg agatattcaa acaggccatc
     2341 gcgaacctcg aacggctcgg cgtcgatgcg ctcgtcgccg tcggcggcga gggcacgctc
     2401 gccatcgcct cggagttcga gcgtcgcggc ttccacgtcg tcggcgtccc caaaaccatc
     2461 gacaacgatc tggcggcgac ggagttgacc ttcggcttcg tcaccgcgct cgacatcgcc
     2521 accgaggcgc tcgaccgcct gcacaccacc gccgagtcgc acgaccgcgt gatggtgctc
     2581 gaagtgatgg gccgccacgc cggctggatc gcgctgcact caggcgtctc cggcggggcc
     2641 gacgtgattc tcatccccga gattccgttc tccatggagc gcgtcgccgg gaaggtgctt
     2701 gaacgcgagc gttgcgggtc gcagttcagc atcatcgtcg ccgccgaggg cgcgtgcgag
     2761 attgcgggca aagaggtcta tcaggacgcg ggcagccagc agcactcacc gcgcctcggc
     2821 ggcatcggcc accacgtcgc ggcggagctt ggggagcgga cgggcaagga ggcgcgctgc
     2881 gtcgtgctcg ggcacctcca gcggggcggc agccccaacg ccttcgaccg gatgctcgcg
     2941 accaacttcg gctcggctgc cgtccgcgcc ctcgttcgcg gcgagcgcgg cgtgatggtc
     3001 gccctccaag ccgccaacgt catcaccgtc cccatctcac acgccatcgc caacctcaag
     3061 accgtctccc ccgacagcca actcgtccgc accgcccgcg acatcggcgt ctccttcggc
     3121 gctacggacg aggcgcggca tcacaagtac gaagtctgct gaaaagaagg gagtcaggag
     3181 tcagaagtca gaagtcagaa tgaaaagcgc gctcgccttc ttcattctga ctactggctt
     3241 ctgactcctg actccctatt ctggcttctg gcttctggct cctgactccc tcgccttgtc
     3301 agactacgca gagttcttcg aggaattgca ggtcgagttc ctgcggctca tgttgctcgt
     3361 gtgtgctgag gtagtcgaag agggtgccga cgaggacggg gtcgaactgc gtgccgacgc
     3421 catggcgcag acgctcgacg gcgacggcga gcggcaggcg cgcgcggtag ggccggtcgc
     3481 tggtcatcgc gtcgtaggcg tccacgaccg agatgatgcg cgcgcctaag atgatctcct
     3541 cgccggcgag gcccgccgga tatcccgtgc cgtcccacca ctcgtggtga tgcgagatca
     3601 tcgagcgcac ctgctccatg tgcggcacgc cggagagcgc ctgctcgccg tggtagacgt
     3661 ggcggcgcat cacgtcctcc tcgtccggcg tcagcgcgcc cggcttgcag agcactgtct
     3721 ccggcacgac gatcttgccc acgtcgtgca agagcgcccc cagcttgacg tacatcgcct
     3781 cctcttccga cagcccgcac tggagggcga ggccctcggc cagacgcgcc accgactgcg
     3841 agtgctgccc cgtgtagctg tccttggcga agagcaggcc gatgagcgtc ttgaccgtgg
     3901 cgacgaaggc gcggcgcgct tcgagttcga ggacgcggtt gcgctccttg atctggctga
     3961 ggactaaaac gttgtcgatg atgttcaaaa gctcgcggta gccgaagggc ttgtggagat
     4021 aggcggccag tcccatctcc atcagctcgc gctgctcggc gtcgttattg gtgcgggccg
     4081 tgagcatgat gacgggcgtg cgccggagct gcgcgtccgt gctggcgacg agttcgcgga
     4141 acacttcttt cccgctgatg tcgggcatca tgtaatcgag gaggatgatg tcgggtcggg
     4201 aggcgcgcgc atagcgcagg ccctcgcgcc cgtcgagcgc gccgaggaac tggtagccgg
     4261 cgcgctccag cgtcaaccgc cccagtcgca gcagcgacgg gtcgtcgtca atgaagagga
     4321 cgagttttct tttctccgaa ggtgttctga tcacggcgtg ttgcggcacg cctccccggc
     4381 gcctccgtcg ggtgcggcgc gctccctccc ttcatggatg gcgaacgagg ggccgcgagg
     4441 taggcccaca gacaaaggga cgcgcggctc gtctgggcac aaattacagg agaggaggtt
     4501 cgagcggcgc gggcgccgat tcttgcggag gcgtggcaag ctggcaggga gaccgaactc
     4561 aaaaaaggct tcggagcttg cccccgtttc cgaaatgacg ccggacgaag tatattccag
     4621 tggcgacttt taataccatt gcgttttggt tacattttgg cagaggcgtt gatatggtcg
     4681 gagactttgg gacgtgactg aagtaatcaa gcaaaagctg cgcgacgaac tggagacgct
     4741 tgagtcggag ctgcgcgtac acctgccgcg cgagatcaag cgcgcgctcg aattcggcga
     4801 cctgcgcgag aactccgagt acaaggcggc gctcgaacgc cagagcttcg tcagggcgcg
     4861 catcggccaa ctgcgtcagc gcatcagcga aatctcctcc atcgacatct ccaacatccc
     4921 gcgcgaccgc gccgcctacg gctccaccct ggtcctctac gaccccgagc gcgaagagga
     4981 gctgacctac cgtctcgtca cctcggagga gagcgacccg gcggccggac tcatctccac
     5041 cacctctccc atcggccgca gcctgatggg ccgcgaggag ggcgacgagg tcaaggtcgt
     5101 gacgccggcc ggcgcccgcg agttcgagat acgcagcctg cgcaccctgc acgacgaggc
     5161 ggcggaagaa aaaaaggatg agggatgagg gatgagggat gaaagaagat agtaagcagt
     5221 gagcagtaag cagtaagcag tcaaggccgg tcgagcgttc tgactgctca ctgcttactg
     5281 cttactccct ttcatccctc atccttgctt ccgcgcgagc ctcaaggctc ttcgtcctct
     5341 tcttcctctt ccgcttccgt cttcggtttg tcgccctcca cggcgccatg tgcgacgagc
     5401 aacgccgtca cttcttcata ttcgcctccc cttgcgaagt cgagcgcggt cttgccctcg
     5461 tcgtccttca ggttgtagtc ggcccccgcc gccaccagcg ccctggcgtt ctccaactcg
     5521 cccacctccg ccgcgagcat gagcgccgtc cggccctcgt cgttcctcat gttgattttc
     5581 gcgcccgcgt cgagcagccg gcggacgact tcggcggtgc ttctggcggc cgcgtgggcg
     5641 agcgcggagt tgccgtcgtc gtccctcact ttgacctcgg ccccggcgtc gagcagggcg
     5701 cgcacgaggt cgggcgtcgc gtcttcgtcg agcgtcatca gggccgtgcg gcccgccctg
     5761 tcctcggcgt tgatatcggc cccggcgtcg agcagggctt tgaccatctt caggttcgcg
     5821 tgggcgacgg cctgtgcgag cgccgtcgag tcgatgcctc gatcaaggac gttggcgtcg
     5881 gcgccgcgcg cgaggagttt ttccacctcc gccagatcgt ctttgaacgc cgccttcacg
     5941 agcggctcgc tcggttcggc gaccatcacc attacgcccc ccgtgatttc gccgatctga
     6001 agggtcgcgt ccacgcggcc ctgtcccccg ccctgcacgg agacgttcgc ggcctcgtag
     6061 tcgcggaagc cggcggccgc gaaccggagc gtgtaagttc cgggcggcac attactgaaa
     6121 gcgtattcgc cctcgtcgtt tgaagacgcg gttcgggtct ccccggtctg tgtgttgctg
     6181 agcgtcacgc tggttccggc gacgcgcgcc tgtagtgggt ccgtgactgc ccccgcgagc
     6241 gtcgccccgc tcggctccaa cgcatcacgc ccggccgcgc gcgccgcgac gttgatttcg
     6301 acgccgctcg cggactgccg gccggacggc gactgcgtct gcgcggcaac gctcgcgccg
     6361 aagctgatgg cggcggcggc ggccccggcg gcgaggcgcg aggcgcggcg cgcgatgtgg
     6421 tagagcgccg gcgccgcgtc ggccgtgagg acgccgccgc ccggcagttg cacgtacctc
     6481 acgcacaggc gcccgcccga gttggcgacg agcctcgtcg cttccttccg cgtcatgcgc
     6541 gagaggtcgt tgacgctcag gcggcagtgt tcgcagaagc gcacgcggtc gttgccgctc
     6601 atcgagtccc agtcggcgga acagggcttg gggacgcgga ggcgttcgag aagatcggtc
     6661 ttgcggcgta gcacaggcgt tctcctttcc aaccgtcgtt gttgcggaga gccgggggag
     6721 agacgctctt cggtcattga aacgtgcgag gcgtgggagc gcgccgcgtc gggcgtgccc
     6781 tttgcgcctc tgatgtgggc gcgccgcgac cgggttgctg cggcgtcgga ttttttatgg
     6841 acgtgcgggg cgcacgggga tgcggccacg cgcacgcgcc gcaagtcgcg cgttcttcgc
     6901 gcgcccttcg ggcgacttgc ttgacaagca tttcggcgcg ttcctatgat gcagggctga
     6961 cgcggcgcgc tgggcgcgcg gctcaccaag tcggctcgcg agagacgaac tccttcccaa
     7021 tctggacgca agcgaaagag gcgattaaat gttcggggcg agcatcggcc gcggcgcgca
     7081 gcggatcatc ggcgcgatgg tgcgggggct gtcgcgcagc tcgatcaacc ccaacgtgct
     7141 gacggtcatc ggcgtctcga tcaacgtctt tagcggagtc ctcttcggac tcggacactt
     7201 cttctgggcc ggcgtcgtcc tcatcgtcgc caatctcttc gacatgctcg acggtcaggt
     7261 ggcccgcctc acgggtcgcg tcacgcgctt cggcggcttc ctcgactctt cgctcgaccg
     7321 cctctccgac atggccgcct tcgtcggcct gatggtcttc tacgcgcgct acaccgacga
     7381 ccccgcgagc ctcgcctacg gccagcactc tcccttaaac gtcttcctcg ccggcgcggc
     7441 gctcgccggc tccgtgctcg tctcctacgc cagcgcgcgc gccgaaagtc tcatcccgaa
     7501 gtgcgacgta ggcttcctgc gacgccccga gcgcgtcgtg ctgctcatca tcggcggcct
     7561 ctcgacgcat cccggatgga gcagtccctt cgccaaccgc atgcccgccg tcctctgggt
     7621 gctcaccgtc ggctcctact ggacgttcgc ctaccgcatg tatcacacgt ggagcgagct
     7681 gcaacgaacg gagggcgcga cgacgagcaa cgccgctgcc tctgacacga cgccgcgcga
     7741 gacggtcggc gagcagactg ctacggcaca gacagcggcg gggccggaca cgccgcaagc
     7801 atctgcgggg gccggcgggg cgtctaacgc gggtgcggga gaacggcacg gcacggccga
     7861 tgtcgtccga cgcccgcagg cttcgggctg agggtcattg atgagcgaca acagacgctc
     7921 ctccgcgaag gtgagcgaca acagatgttc tttcgcgggt cgcgagcgca agcgcgcggc
     7981 cttgtgtcct tgctgcggcg caggcgtcga gggcgacatg gaagcggggt gcgcggggtg
     8041 cggggcgcgt cgcgtcggcc cgccgctgcc gcgcccggag cgagagctgc ccgcttacgg
     8101 tcacgccttc ttctccgccg cgcttggtct cctgttagcc tccgccttcg tcgtcgccac
     8161 ggcccttgcg ctcttcgagc gcgacgcctt cgcgctcgga ctcggcgcgc ttctggccgc
     8221 cgccgagcgg gccgcgtggc gtttgaagtg gacgcttctg ccggccgcgt tcattgccgc
     8281 ctgggcgtgc gcgcgtctga accggcacat gcgtcgcgcg ccgtcgcgct tcgtcggcca
     8341 cggatttgcg cgcgccggtc tggcgctctc gtgtgcgtcg gcgctcgccc tcgccctgtt
     8401 cgtcgtcgcg tctgttcccg aacgcctgcg ccgtcgcgaa ctcgggcggc gggccggcga
     8461 ggaggccacg ctttacgcga ccgaccgcgt gctcttcgcc taccgcgcgc gcttcggcac
     8521 ttatccgtca gcgcccgccg acttgcgccg gctgcccgac cccgacggct ccgtcgccgc
     8581 cacagtggcc ctcttgcaat caggcagcta cgcccccgag gccgaggtcg cctcgctcgc
     8641 gccgaagggg cgcggccgtc gccgcgcggc cgcgccgcgt ctgcgtagcg tgtccgcgcg
     8701 tggccgcgac gactctgcgg gcgcgggtct gtctctgacg agctacgagc tgacgctgcc
     8761 gggcagagac aaggtgctgg gcacggccga cgatcttcgc ctgcgcgacg gcgtgccgct
     8821 caaggcgggc gaccctccgc ggcggctggc gacgccgacg cccgtctgag actgccgacc
     8881 gccacgtcaa tatcgatcat gggaatcttc gacatcttcc ggcgcagaaa atcggacgac
     8941 gaggcgtcgc gccgcgcgca actcctccgc acgggtcgca tcgccgaagg ctccatcttc
     9001 gacgtggtca ccgacgaggc gggccacgtc acccaaatct tcttcaacta caccatcaac
     9061 ggcgtcgatt acgagtcctc tcagttgctc gacccagggc aacttctccg tcagggcgac
     9121 tacttccccg gcgcgcgcgt caccatccgc ttccacccac aacgccccgg caactcggtg
     9181 gtggtctaaa gacagaggtc agaggctaga ggttagaggt cagtcaggag cttgccttta
     9241 ctgacctcta acctctaacc tctgacctct actgcttatg tttcgcaaaa tcctcatcgc
     9301 caaccggggc gagatcgccg tgcgcgtgat gcgcgcgtgc cgggagatgg acatctccac
     9361 ggtcgccgtc tactcggagg cggacgccgc ggccttgcac gtcaggctcg ccgacgaggc
     9421 ttatctcctc ggccccgcgt cgtcggcgga aagctacctg cgtatcgacc gcgtgattga
     9481 cgcggcgctc cgctccggcg ccgaggccgt ccaccccggc tacggcttcc ttgcggagaa
     9541 cgcgcagttc gcgcgcgcgg tcactgaggc cggactcacg ttcatcgggc cgcccgccga
     9601 ggtgatggaa ttgatggggt cgaagacgag cgcgcggcgc gcggcgacgg aggcgggcgc
     9661 gccggtcgtg ccggggacga ccgaggcgct cgcgtcgctt gcggagctta aggagacggc
     9721 ccgtgggctt ggctaccccg tgatgctcaa ggcggcggcc ggcggcggcg gcaagggcat
     9781 gcgcttagtc gagacggaag cggaactcgc gtccagcttc gaggcggcgc gctcggaggc
     9841 ggcttccagc ttcggcgacg atgcggtcta tctggagaag gccgtcgagc ggccgcgcca
     9901 catcgaaatc caaatcttct ccgacgcgca cggcaaccac ctgcatctcg gcgagcgcga
     9961 gtgctccatc cagcgccgcc accagaaggt catcgaggaa gcgccgtcgc cgattgacga
    10021 ccccgtgttg cgcgcgtcga tgggcgaggc ggccgtgcgc gtggcgcgcg cctgcgccta
    10081 cgtcggggcg ggcaccgtcg agtttttagt ctccgacgta acgcgcgagt tttactttct
    10141 ggagatgaac acacgcttgc aggtcgagca cccggtgacg gaactcgtca cggggttcga
    10201 ccttgtgcgc gagcagatac gcgtggcggc gggagagccg ttgtcgttcc ggcaggagga
    10261 cgtggtgttg cgcggacacg ccgtcgagtg ccgcgtctac gccgaagacc cggagaacaa
    10321 cttcctgccg tcgcccggcc gcatcacgca cctgcgcgtg ccggcggggc cgaacgtgcg
    10381 cgacgacggc ggcgtctacg cgggcgcgga ggtctcgatc cactacgacc cgatgatctc
    10441 gaaactcgcg acgtggggcc gcacgcgcgc cgaggccatc gcggcgatgc gtcgcgcgct
    10501 cgacgagtac acggtcgccg gaatcaagac gacgattccc ttcttccgcc aggtgatgcg
    10561 cgatccggaa tttctcgacg gccgactcga caccggcttc atcgcgcgct tcgaggagcg
    10621 ccgccgcgcc gcgctcgacg ccgaagagaa cgcgggcgag gcgaaggatg aagaggtcgt
    10681 gcggcgcgac atggcggccg tcgccgccgc gctcgactac gagcgacgac aacggcagca
    10741 ggtgtcttcc gcggacgagg gccgcgcgcg ggagtcgagc gcgtggaaga tgtcgggccg
    10801 cgccgcgctc ctccagtcgc gcgtcaggtg atgggccgcc ccggcctatt gtctgaggcg
    10861 cagggtgtgc agacgcggcg aaggcgtctg cgcgcttccg cgcgacaagg tgacttcgac
    10921 gtagtagaga ttgttggtga agtccaccgc gctgttggcg aaggtgcagc gcgcgcacgg
    10981 ggtgtcgccg ctccggttgc gcgccgtgca gatgagcacc tgctgctggt cacagcttct
    11041 aatctggaac aggctggcct cgatgatggt gctgttcggc gtagtggaac ccatctccaa
    11101 cctcgtccag cccggacggt tcgggttggc gttgttgtcg aaggtgttcg tcacattgta
    11161 gcgggcgacg atcttgaggg cgttggcgcc ggcccggaag ccgaggtcgg tgttggagaa
    11221 ggcgaagaag ttgagcgagt cttcgtctat gacgccggtc gagccaacgg ccgtccacgg
    11281 cttcgggaga acttggaacg cgggttcggc tggttcggac accggcgcga ccgcggaact
    11341 gcggccggcg ggcgacgcga gcatcagtgc cgcgagcgcg ggcacgacga agagcaggcc
    11401 gagccgggtc aggccccggc cctgtcgcaa gacgttctgt gtcatgggaa gcacctccgg
    11461 tgtgggaacg cccgccgaaa gacgttcggc gcaggcgggc gtcgcgtgtt tagtgagagt
    11521 gagcggcgag gcggcgacgg ttacggggcg ggtgcggcgc ttggtgagag ccggaaccaa
    11581 aggtcacgcg gtacgcgtgg cttatacatc ggcctccacg cgctgtcaat aaaacgttcg
    11641 gcggaaggcg cccccggtga gcgcgccggg ccggcgtact cgacagcctt tgtcttatga
    11701 aactaatagc tgacatcgac ggcgagcgtc acacgctcga agtggggcgc gagggcggcg
    11761 ggcgcgtcgt cgccgaagtg gacggccgcc ggcacgaact cgaagcgagt gaggtggagc
    11821 cgggcgtcta cctcttcgtc cgcgaggggg aagtgtttga gatacacctc ggcggcgcgg
    11881 cgggcgcggc gggcgcgtcg ccgccggaaa tcagcgtccg acatcgtggc gggcacggcg
    11941 cgtatgccgt cagggtgatc gacccgaagc gactgcgcgg ggcgcgcgcc gccgccggcc
    12001 acgacgcggg gcgcgcgcaa atcacggcgc cgatgccggg caaggtcgtc cgcgtcctcg
    12061 tcgagctggg gcaggaggtg gaggccggtc agccggtcgt cgtcgtcgag gcgatgaaga
    12121 tgcagaacga gttgaagtcg ccgaaggcgg gcgccgttgt cgcgctgcac gccgcggtgg
    12181 gcgcgaccgt caactcgggc gacatcctcg ccgagatcga gtgagggatg agggcggagg
    12241 gatgaatgaa aggctgttcg actcatcgct taatgtgttt gctcgaactg aggatggcac
    12301 ttgaaccgac aactcgacgc ctcatccctc atccctccgc cctcatccct cgcagacctg
    12361 cgcgtcctgc tgtgggacat cgacgggacg ctgatccgtt cgcgtcgcac gggggcgttc
    12421 aaggagtaca cgatccccgt gctggagagc gtgttcggga cggcggggcg gctccgggag
    12481 ttgtcggtct cggggatgac cgacttgcag atcgtcgccg agtctctgcg cgacgaaggg
    12541 ttcacgcccc gcgagattcg cgcgcgcctg cgcgagctga gcgcgctcta cctcgcggcc
    12601 ctggagcgcg ccgccggcgc ccgggaaggg ccgcctctct tcgaggtgct ccccggcgtg
    12661 cgcgaagtcc tgacggccgc gcaggcgcac ccgcgttacc tctccgcact gctgacgggc
    12721 aacatcgagc cggccgcgcg tctcaagatg cgcctcgtcg gcctcgccga cttcttcaca
    12781 ctgcccggcg ccttcggcga cgactcgcac gaccgccgcg acctgcccgc cctcgcgagc
    12841 gagcgcatca accgacacct cggcctcgcc ctgctccctt cacagttcat cgtcatcggc
    12901 gacacgccaa acgacatcgc atgcgcccgt cacttcggcg cccgcgccat cggcgtcggt
    12961 acgggccgcg tcaacagcgt cgagacgctg cgcccacact ccccggacgc gctcctgccc
    13021 gacctctcgg acacaaaccg cgtgatggaa gttctcgcca gactataatc agagagggga
    13081 aagtgaaaga cgaaagcggg aagcaattcc tccccacttt cgtctttcac tttcccctct
    13141 aggt
//
LOCUS       DASVES010000399         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1284081, whole genome shotgun sequence.
ACCESSION   DASVES010000399 DASVES010000000
VERSION     DASVES010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284081"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..973
                     /locus_tag="VF240_16440"
     CDS             182..973
                     /locus_tag="VF240_16440"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7176852.1"
                     /translation="MKICRFISDAFEGPRFGIVEGSEVLPLPAGFSLREMSGEAADAR
                     TERPVSIASVRLLAPVTPSKIVCVGRNYREHAAELGNPMPEEPLLFMKAPSALAATGD
                     FIELPPASQRVEHEGELAVVIGRRARRLNDSDDPLACVLGYACLNDVTARDLQRRDVQ
                     FTRAKSFDTFCPVGPHVATGIDPLDLKVETRVNGETRQSGRTSQMAFPVPHLVRYISH
                     CMTLEPGDLIATGTPAGVGPLNDGDVVEVEVEGVGVLSNPVRHAD"
     gene            1014..1661
                     /gene="fsa"
                     /locus_tag="VF240_16445"
     CDS             1014..1661
                     /gene="fsa"
                     /locus_tag="VF240_16445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX7176853.1"
                     /translation="MKFFLDTASLQEIRDAQQMGLIDGVTTNPSLVAKEGDVEFKQHI
                     AAICEITQGPVSAEVTSIDTEGMLREGREYASIAPNVVVKCPLTKDGLRATRTLTDEG
                     TRVNVTLCFSAAQAILAAKAGAAYISPFVGRLDDIAHDGMQLIRDIIEIYDNYDWYTE
                     VLVASIRHPLHVVDAARAGADVATLPFKVLEQLWKHPLTDRGLEQFLDDWRKSGR"
     gene            1685..2203
                     /locus_tag="VF240_16450"
     CDS             1685..2203
                     /locus_tag="VF240_16450"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX7176854.1"
                     /translation="MAGVAIDEESAGRTVFVESRKYDGRVHRSWNAHLLRVEGALIVV
                     EGTFEEEIVHPNLGVIEPGTSSREFYWTDRWYSVFRFQRPAGELRNYYCNVNEPARFD
                     GRTLRFVDLDIDLLVAPDMTYTVLDEDEFAEHAVRFKYPPETRERARAALAELIDLVT
                     RRQFPFGAGAKR"
     gene            2200..>3228
                     /locus_tag="VF240_16455"
     CDS             2200..>3228
                     /locus_tag="VF240_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7176855.1"
                     /translation="MKPKPTPVPATKIERLRERLRRAAEGGGPERVERQHAAGKWTAR
                     ERVEFLLDEGTFEEFDQLVVHRSRDFELDKQLYPGDGVVTGHGRVDGRRVFVFAQDFT
                     VFGGSLSETHAEKICKVMDMAMKVGAPVVGLNDSGGARIQEGVVSLGGYADIFLRNTL
                     ASGVVPQISCIMGPCAGGAVYSPAITDFNVMVRDTSYMFITGPEVIRAVTHEDVTKEE
                     LGGADTHNRVSGVAHFAADTDEHALRLTRELLSFLPSNNMEEAPRLTTSDPSERADER
                     LNTLVPEASNQPYDIRDAIHPVIDDGYFFEVQPHFAPNIVVGFARLAGRSVGIVANQP
                     AYLAGVLDI"
BASE COUNT          602 a         1146 c          955 g          525 t
ORIGIN      
        1 aatctccctc tggacggctg gacgaagctc aatcgcacgc tgctcgaccg cacgacctat
       61 caggatgact ccgtgcagtc gggcatcaaa tatttttatt acctgacggc cgtagactcc
      121 gccggcaacg tcagcaagcc ctcggacgtg gcttccgaaa ccgtcccctg aaatggctgc
      181 catgaagatt tgtcgtttca tcagcgacgc ctttgagggg ccgcgcttcg gcatcgtcga
      241 aggctccgag gtgcttcccc tcccggcagg attctctctt agagagatgt ccggtgaggc
      301 ggccgacgcg cgtaccgaga ggcccgtgtc aatcgctagc gtgcgactgc ttgcgcccgt
      361 cacaccctcg aagatcgtct gtgtcgggcg taactaccgc gagcacgccg cggagcttgg
      421 caaccccatg ccggaagagc cgctgctctt catgaaggcc ccttccgcac tcgccgccac
      481 cggcgacttc atcgagctgc cccccgcatc tcagagagtc gaacatgaag gcgaactcgc
      541 cgtcgttatc ggacggaggg cgcggcgtct caacgactcc gacgacccgc tcgcttgcgt
      601 cctcggctac gcctgtctca acgacgtgac cgcccgcgac ctccaacgcc gagacgtgca
      661 gttcacccgc gccaagtcgt tcgacacctt ctgccccgtc ggcccacacg tcgccaccgg
      721 catcgacccg ctcgacctca aggtcgagac acgcgtcaac ggcgagacac gccagagcgg
      781 tcgcacgagc cagatggctt tccccgtccc gcacctcgta aggtatattt cgcactgcat
      841 gacgctggag ccgggcgact tgattgccac cggcaccccg gccggcgtcg ggccattgaa
      901 cgacggcgac gtggttgagg tggaggttga aggcgttggc gttttgagca accccgtcag
      961 gcacgcagac tgaggcggcc tgattcgttc tcgcagagtg agaaaggtca aagatgaaat
     1021 tcttcctcga caccgcgagc ctccaggaga ttcgcgacgc tcagcagatg ggactcatcg
     1081 acggcgtgac gaccaacccg tcgctcgtcg ccaaagaggg cgatgtggaa ttcaaacaac
     1141 atatcgccgc catctgcgag atcacccaag gcccggtctc cgccgaggtg acgagcatcg
     1201 acaccgaagg gatgctccgg gaggggcgcg agtacgcgtc aatcgctccc aacgtcgtcg
     1261 tcaagtgccc gctcacgaag gacggcctgc gcgccacgcg cacgctcacc gacgagggca
     1321 cccgcgtcaa cgtcacgctc tgcttctcgg ccgcgcaggc tatcctcgcc gccaaggccg
     1381 gcgccgccta catcagcccc ttcgtcggcc gactcgacga catcgcgcac gacgggatgc
     1441 agctcatccg cgacatcatc gaaatctacg acaactacga ttggtacacg gaagtactcg
     1501 tcgccagcat ccgccacccg ctccacgtcg tcgatgccgc acgcgccggc gccgacgtgg
     1561 cgaccctgcc cttcaaggtg ctcgaacaac tctggaagca cccgctcaca gaccgtgggc
     1621 tggaacagtt tctcgacgac tggcgaaaga gtggccgcta aatcccgctt cacacggagg
     1681 acacatggcg ggggtggcaa ttgatgagga gtcggccggg cgcacagtct tcgtcgagtc
     1741 gcgcaagtac gacggacgcg tccaccgctc atggaacgcg catctgttac gcgtcgaggg
     1801 cgcgctcatc gtcgtcgaag gcacattcga ggaggagatc gttcatccca acctcggcgt
     1861 catcgagccg gggacaagca gccgggagtt ctactggacg gatcgctggt acagcgtctt
     1921 ccgctttcag cggcccgccg gcgagttgcg gaattattac tgcaacgtca acgagccggc
     1981 ccgcttcgac ggccgcactc tccgcttcgt cgatctcgac atcgacctcc tcgtcgcccc
     2041 cgacatgact tacaccgtcc tcgacgaaga cgaattcgcg gagcacgccg tgcggttcaa
     2101 atacccgccc gaaacgcgcg agcgggcgcg ggccgcgtta gctgagctga tcgaccttgt
     2161 cacacgccga cagttcccct tcggggccgg agccaaacga tgaaaccaaa gccgacgccg
     2221 gttccggcaa ccaagatcga gcgcctgcgc gaacgcctgc gccgcgccgc ggagggcggc
     2281 gggccggagc gcgtcgagcg ccagcatgca gccggcaagt ggacggcgcg cgagcgcgtc
     2341 gagttcctgc ttgacgaggg cacgttcgag gagttcgacc agctcgtcgt ccaccgctcg
     2401 cgcgacttcg aactcgacaa acagctctac cccggcgacg gcgtcgtcac cggccacggc
     2461 cgcgttgacg gccgccgcgt cttcgtcttc gcgcaggact tcaccgtctt cggcggctcc
     2521 ctctccgaga cccacgccga gaagatttgc aaagtgatgg acatggcgat gaaggtcggc
     2581 gcgcccgtcg tcggtctcaa cgattcgggc ggcgcgcgca tacaggaggg cgtcgtcagc
     2641 ttaggcggct acgccgacat cttcctgcgc aacacgctcg cctcgggcgt cgtcccgcag
     2701 atcagctgca tcatggggcc ctgcgccggc ggggccgtct acagccccgc catcaccgac
     2761 ttcaacgtga tggtgcgcga cacctcttac atgttcatca ccgggccgga ggtcatccgc
     2821 gccgtcacgc acgaggacgt gaccaaggaa gagctcggcg gggccgacac gcacaaccgc
     2881 gtctccggcg tcgcgcactt cgccgccgac acggacgagc acgccctgcg gctcacgcgc
     2941 gaactgctct ccttcctccc ctcgaacaac atggaggagg ccccgcgcct caccacttca
     3001 gacccgtcgg agcgcgccga cgaacgcctg aacacgctcg tccccgaagc ctcgaaccag
     3061 ccctacgaca tccgcgacgc gatccacccg gtcatcgacg acggctactt ctttgaagtc
     3121 cagccgcact tcgccccgaa catcgtcgtc ggcttcgcgc gcctcgccgg ccgcagcgtc
     3181 ggaatagtcg ccaaccagcc cgcttatctg gcgggcgtcc tcgacatc
//
LOCUS       DASVES010000400         9765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_281655, whole genome shotgun sequence.
ACCESSION   DASVES010000400 DASVES010000000
VERSION     DASVES010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281655"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3305
                     /locus_tag="VF240_16460"
     CDS             <1..3305
                     /locus_tag="VF240_16460"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX7176856.1"
                     /translation="LVYTFTRTGDTTGALVVNCTVAGTADSSTDYTQTGATAFTPPTG
                     TVTFSAGNSTATVTIDPTLDSTAEPNETVVLTLAAGADYNVGTPSAATGTIDNDDTAV
                     SVAVSPGAVDEDGATNLVYTFTRTDSSGALTINFSVGGTATFNTDYTQTGAATFTPPT
                     GTVSFADGSLTATVTVDPSADATTESDETVVLTVTSGTGYTVGAPASASGTITNDDTE
                     VTVAVSPLSTTEDGAGNLVYTFTRNGVTTGALTVNFTVGGTADSNTDYGQTGATAFTP
                     PTGTVDFIAGSSTATVTVDPTADNTAEADETVILTVTSGAGYNVGTPSAATGTITNDD
                     AGVTVAVSPSTVEEDGATNLVYTFTRTGFTGAALTVNLTVGGTAAFSTDYTQTGAATF
                     TAIDGTVTFGAGNSTATVTVDPSADATSEPDETVILTVISGAGYEVGSPDSATGTINN
                     DDNAVSVAVSPLNVDEDGATNLVYTFTRTDSTEAQTVNFSVGGTAAFSTDYTQTGADT
                     FGATGTVSFAVGSLTATVTVDPSADTTVEPNETVILTVTAGTGYSVGSPSSATGTINN
                     DDTDVTVAVSPLSTTEDGAGNLVYTFTRNGVVSSGLTVNISVGGTAAFSTDYTQTGAA
                     SFTASSGTVTFTAGNSTAAVTVDPTADGAAELDETVILTVTAGTGYNVAIPNSATGTI
                     INDDTEVAVAVSPSAVDEDGATNMVYTFTRTGIISGALTVSFSVSGTAIFAPPNTDYT
                     ATTTMGDTFTATSGTVTFAAGSSTATITVDPFGDADMEPNETVILTVTAGAGYTVGSP
                     ASATGTILNDDNSPPTVAVGYGQCGGVSTGTLNLAVGDVETPAASLTMSATSSNTAVV
                     PLANISFGGSGPTRTVTITAVPQTAVALSDVTITVNDGTLSSSINIRVIVGTNKSETI
                     SIGTTTVGTDMIFARNGDDTINAGAGNDLVCGGNNVGVINGGLGDDTLDGGNGNDILR
                     GGDGNDLLLGGGGNDTIEGGNNNDTLDGGTGTDTLRGESGDDTLTGGSGPDSFNGGAG
                     NDTATDFNVGQGDTKVDVIEVGALFPALLRSEVFGYLASGNLWLVNPFTDVVTPPSHF
                     MTGDGPLRGLRP"
     gene            complement(3525..4049)
                     /locus_tag="VF240_16465"
     CDS             complement(3525..4049)
                     /locus_tag="VF240_16465"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7176857.1"
                     /translation="MQSSEIRLPLPRGGTVSVRPVTAEDEGFLLTVYAGTREDELAQV
                     EWAEGQKEMFVRWQFEMQRREYDARFPDAEYGVILVDGRPAGRVWVGTDEEQIRLLDI
                     ALLPEAQKRGVGTLLLRRLMDEAARAGKPLRHTVFVLNNDAKRFYERLGFAVIEDLGA
                     YQHMEWRPAATPGS"
     gene            4814..>9765
                     /locus_tag="VF240_16470"
     CDS             4814..>9765
                     /locus_tag="VF240_16470"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7176858.1"
                     /translation="MNKSFPTPRRRSLKAFTAGLISYLLLVGPLAPLALAARPHAPRA
                     GVASPAPESGRRAAPLAAAPAPLAFAPNIVATKVDSFNDLGADGKADPGQTITYTVTI
                     TNNGPDPATGVTFTDMVDANTSLVPGSVQTQPVAVADAFNVIGNVRIQVPDGASDLLA
                     NDRDPDTGNNTGLTASGPTTSTNNGNVTVNADGSFSYNPAPGFTGTDTFTYTITDSGG
                     AMDTGTVTLTVSGLVWFINSAAAVGGDGRLTSPFNCYVGANVPASTTCYSATAVDDPG
                     DNIFLYSGSYTGGDTLQNNQKLIGQGATTTLDAVAGVTVPANSDPLPALNGNPSTVTI
                     TTTAASTNGVNLAAGAANVFTLRGFTVGNTTGIDIASGANFGTLNVSEVTLSGTGRPL
                     SLTSGALAATFNSITATAAPGGQGINLAGLTGTMAVTGGTSITNPGTQCILVGTTTAS
                     INFGNTTCSGGTDGVSLQNNSSGTRTFGTLSVSNNSGAGFLHTGGGGTTVVGATTITN
                     PGTNGVSVAGSTTSVSFGNTSITGSGAAGVLYTGSSGNLSFADLDITPDNNVQAISTN
                     GNAGTLSATSGAIATSGAAALNLNSTVLNLVFDSVSAATNGSANNAINIVGGSGAVSA
                     PAGTITGGTNPAVIVNGGSVSLAYGGGISQSNNAALLSVLGGHTGTLTFQTGTLSATG
                     GTGLQFDNADGAYNFNGTTTLAGGDAGVDILNDSGGAFNFSAATTITSPSGTAFNVNT
                     GAPNVDYNGTITQNTSGQRVVNVDGTTGSTVLFDGTVTGGQNSTGVRINNANGNVTFT
                     ATLTLGTSVARMGSQAVTIAGGSGAKTFGVVSIFTTGASGIGIGATTSTGAITTASGT
                     VDSAGAAAVNIAGTSAASRTPLNVQLTTVNTNAGTNGIILQNTSATGSPGGFRILGTS
                     AGICGGQVTNTTTPSVAATVTAANTADCTGGTITGATGDGIRLNNADRVSLTRVRVIN
                     GAQSGIFGTGVNGFELISSFISNNGNAADEVGLKFTDVINPGTVNGLVGTALNGANPT
                     RIINSTVRQSGEFNVEVVNRTGTLTDLFVDGCQFTDTKSNAGPADADGFNVEMQNTAV
                     ATVRLQNSVFSNNFTQGAQLSAIDSANLTATVTSSTFANNFEGFVCNHSGNSDLVCTV
                     GGDVGTQGNHFEGNPSLAESGALVVLSNGSSATVSASLTGKVKNNHVTAPANMVNHSI
                     LAFISGVGTPSSLNISNNTVNGGAFGNPILVDTPDTGSSPNFSVTANNNVLSGGSSHG
                     VNVTARQSSTSCVLIQNNTGTPPGASAVVRVRQVSPATYNLKQGVSASLVPATVLTDN
                     NPGASNSVAGTINVVSNAACTTPIASLLGVPNRTFSEVASRQTPAATANVTARDSGGT
                     REREALSARASAIDAPASGVSAGGFARADSPAFSDGWADTFLPARTASINGLFASALR
                     YSPSNVAGPLASAYLASPAATAAFSGETVGPINIGTLPATKSVTIKFRVTVDSTVTAT
                     NISNQGTVSGTNFSPVQTDDPDAAGSANPTLTPVDLPDVTVAVSPASALEDGADNLVY
                     TFARQGATTSSLTVNFSVGGTATFSTDYTQTGAATFTPPTGTVVIASGSSTATVTVDP
                     SADSTVEPDETVILTVTSGTAYDVGSPSVATGTITNDDTDV"
BASE COUNT         1788 a         3560 c         2996 g         1421 t
ORIGIN      
        1 atctcgtcta caccttcacc cgcaccggcg acaccaccgg ggcactggtg gtgaactgca
       61 cggtcgccgg gacggccgac tccagcaccg actacacgca gaccggcgca accgcgttca
      121 ccccgcccac cggcacggtg acgttcagcg ccggcaactc gacggcgacc gtgacgattg
      181 atccaacgct tgactccacg gctgagccga acgagacggt cgtcctgacg ctcgctgcgg
      241 gcgccgacta caacgtcggc acgcccagcg cggccacggg caccatcgac aacgacgaca
      301 ccgccgtgtc ggtggcggta tcaccggggg cggtggacga ggacggggcg actaacctcg
      361 tctacacgtt cacccgtaca gactccagcg gcgcgctcac catcaacttc tcggtgggcg
      421 ggacggcgac cttcaacact gattacacgc agacgggcgc ggcgaccttc accccgccca
      481 ccggcacggt gagctttgct gatggcagct tgacggccac cgtgaccgtt gatccgtcgg
      541 ctgacgcgac gactgagtca gacgagacgg tcgtcctgac cgtcaccagt ggaaccggct
      601 acaccgtcgg cgctcccgct tcggcgtccg ggacgatcac caacgacgac acggaggtga
      661 cggtggcggt atccccgtta tcgaccaccg aggacggtgc ggggaacctc gtctacacct
      721 tcacgcgtaa cggcgtcacc accggcgcgc tcacggtcaa cttcacggtc ggcgggacgg
      781 ccgactcgaa caccgactat gggcagaccg gcgcaaccgc gttcaccccg cccactggca
      841 cggtggactt catcgccggc agctcgacgg cgaccgtaac ggttgacccg acggcagaca
      901 acacggccga ggcggacgag acggtgatcc tgaccgtgac ttccggcgcc ggctacaacg
      961 tcggtacgcc cagcgcggcc accggcacca tcaccaacga cgacgcgggc gtgaccgtgg
     1021 cggtgtcgcc gtccacggtt gaggaagacg gggcgaccaa tctcgtctac acgttcaccc
     1081 gtaccggctt caccggcgcc gcgctgactg tgaacttaac ggtcggcggg acggccgcct
     1141 tcagcaccga ttacacgcag accggcgcgg cgacgtttac cgccatcgac ggcacggtga
     1201 cgttcggcgc cggcaactcc accgcgacgg tgacggttga cccgtcggcg gacgcgacga
     1261 gcgagccgga cgagacggta attctgaccg tgatttctgg cgccggctac gaggtcggct
     1321 cgcccgactc ggccacgggc accatcaaca acgacgacaa cgccgtgtcg gtggcggtgt
     1381 cgccgttgaa tgtggatgag gacggggcga cgaacctcgt ctacaccttc acccgtacag
     1441 actccaccga ggcgcagacg gtgaacttct cggtgggcgg gacggccgcc ttcagcaccg
     1501 actacacgca gaccggcgcg gacaccttcg gcgcgaccgg cacggtgagc ttcgccgtcg
     1561 gcagtctgac ggccaccgtg accgtcgatc cgtcggccga caccacggtc gaacccaatg
     1621 agacggtgat cctaacggtg acagcaggga ccggctacag cgtcggctcg cccagctctg
     1681 ccacggggac cattaacaac gacgacactg acgtgacggt ggcggtatcc ccgttgtcga
     1741 ccaccgagga cggtgcgggg aacctcgtct acaccttcac gcgtaacggc gtcgtcagca
     1801 gtgggctgac ggtgaacatc tcggtcggcg ggacggccgc cttcagcacc gactatacgc
     1861 agaccggcgc ggccagcttc accgcatcgt ccggcacggt gacgtttacc gccggcaact
     1921 cgacagcggc ggtgacggtt gacccgaccg ctgacggcgc ggccgaactc gacgagacgg
     1981 tgatcctgac ggtgaccgcg gggacgggct acaacgtggc gattcccaac tcggccaccg
     2041 gcaccatcat caacgacgac acggaggtgg cggtggcggt atctccgtcc gccgtcgatg
     2101 aggacggcgc gaccaacatg gtctacacct tcacgcgcac cggcatcatc tcgggcgcgc
     2161 tgacggtcag cttctcggtc agcgggacgg ccattttcgc ccctcccaac accgactaca
     2221 ccgcgacgac gacgatgggg gacaccttca ccgcgacttc cggcaccgtg accttcgccg
     2281 ctggcagttc gacagcaacc ataacggttg acccattcgg ggacgcagac atggagccca
     2341 acgagacggt gatcctgacg gtgaccgcag gcgcgggcta caccgtcggc tcgcccgctt
     2401 cggcgaccgg caccatcttg aacgacgaca acagcccgcc gaccgtcgcg gtcggttacg
     2461 gccagtgcgg cggcgtgtcg accggcacgc tgaacctcgc ggtcggggac gtggagacgc
     2521 cggcggccag cctgaccatg agcgccacct cgtcgaacac cgcggtggtg cccctcgcca
     2581 acatctcgtt cggcggcagc gggcccactc gcactgtaac gatcactgcc gtaccccaga
     2641 ctgccgtcgc gctctcggac gtcaccatca cggtgaacga cggtacatta tcgagctcga
     2701 tcaacatcag agtcatcgtc ggcaccaaca agagcgagac gatctccatc ggcaccacca
     2761 ccgtgggcac cgacatgatc ttcgcccgca acggcgacga cacgatcaac gccggggccg
     2821 gtaacgatct ggtctgcggc ggcaacaatg tcggcgtgat caacggcggt ttaggcgacg
     2881 acacgctcga cggcggcaac ggcaacgaca tcctccgggg tggggacggc aacgaccttc
     2941 tcctcggcgg gggtggtaat gacaccattg agggcggcaa caacaacgac acgctcgacg
     3001 gcgggacggg caccgacact ctccggggtg agagcggcga cgacacgctg accggcgggt
     3061 caggccccga cagcttcaac ggcggggcgg gcaatgacac ggcgactgac ttcaacgtcg
     3121 gccagggaga caccaaagtt gatgtcatcg aggtgggcgc gctcttcccg gcgctcctga
     3181 gaagtgaggt gtttggctac ctcgcctccg ggaacctgtg gctggttaac ccattcacgg
     3241 atgtcgtgac tcccccgagc cacttcatga ccggcgacgg ccccctccgc gggctgcggc
     3301 cgtagagtcg aaacagtacc gcccgtgacg cgagccagac ggcggcacgg gcggaaataa
     3361 aaggggcggc cggcttctca accacaagcc ggccgcccct cgtttttact cgacacaatt
     3421 cccggcctgg gccgtcacac cctatcgacc tgacctgcgg cggcgacggc cgcgacacct
     3481 gaacggcggc aacttatgcg ccggcgttcg tggctgcgtc gtcttcaact gcctggagta
     3541 gccgccgggc gccattccat gtgctggtaa gcgccgaggt cttcgatgac ggcgaagccg
     3601 aggcgctcgt agaagcgttt ggcgtcgttg ttgagtacga agaccgtgtg gcgcaggggc
     3661 ttgcccgcgc gcgcggcctc gtccatcagc cggcgcagga ggagcgtccc gacgccgcgc
     3721 ttctgcgcct ccggcagcag cgcgatgtcg agcagacgaa tctgctcctc gtccgtgccg
     3781 acccagactc tcccggccgg ccggccgtca accaggatca cgccgtactc cgcgtcgggg
     3841 aaccgcgcgt catactcgcg ccgctgcatc tcgaattgcc accgcacgaa catctccttc
     3901 tgaccctcgg cccactcgac ctgcgcgagt tcgtcctcgc gcgtgccggc atagaccgtg
     3961 agcaagaatc cctcgtcctc cgccgtcacg gggcggacgc tgaccgtgcc gccgcgcggc
     4021 agggggagcc ggatttcgct actctgcatc ggagaatcgt tcctcgaaag cttgcgggac
     4081 gcccgcaccc ttctgtccgt tcgggccggg gccgtccctc ccgttacgcc gacagtctat
     4141 tgtacccgcg caaagactga caactcgttg cacgtattct tcctcgacgg aaaacaaagc
     4201 cgccgcccgg agcccggcgc gtaacttgtc gagccgtcgg cgagcgcaga accagatttc
     4261 tgtgaatgag ccggccccgt aatcaatctg tccttgaaaa gaccggttac aaccgttaca
     4321 atcacggctc ctgagttctt tgttagctca gaagaccgtt agttcctttc agcaattacg
     4381 acagccgtac agcccggcgt ggcccttcgc ccgctgcacc ctttgaccct tatgcttacg
     4441 agccgaggcg ggcctcgccc tcattctgca cctcggtgtg tgctctttcg ccctcgcgcc
     4501 gggcgctcct ggtctccatg tcagcccagt ccgtggcgcc ggccggcggc gaccagtcgc
     4561 acaacaacat gatgccgtgc ctgaccttct acttcaacat cgccctccag ggcgtcttcc
     4621 cgccgagagg ttaaggcgga cgaataatca ggcggggcgg cccgccgacc aaagagctgc
     4681 tcccatcaat aaaagatact cctgttgatc tctgatgtcg tcaaccaacg atgtcgccaa
     4741 ccaacccctc ccctcactct atttggtctc tcgcccgcgc cacgcgcggc cagaaggatt
     4801 ctgaaggaga gttatgaaca agtcgttccc cacaccccgc cgccgctccc tcaaggcgtt
     4861 taccgccggc ctcatctctt acctcctgct cgtcgggccg ctcgcgccgc tggcgctggc
     4921 cgcgcgcccc cacgcgcccc gtgccggcgt cgcgtcgccc gcgccggagt caggcaggcg
     4981 cgccgcgccg ctcgccgccg cgcccgcgcc cctggcgttc gcgcccaaca tcgtggcgac
     5041 gaaagtggac tccttcaacg acctcggcgc ggacggcaag gccgaccccg ggcagaccat
     5101 cacctacacg gtgacgatca ccaacaacgg ccccgacccg gccaccggcg tcaccttcac
     5161 cgacatggtg gacgccaaca cgtcgctcgt ccccggctcg gtgcagacgc agcccgtcgc
     5221 cgtcgctgac gccttcaacg tcatcggcaa cgtccgcatc caggtgcccg acggcgcgtc
     5281 agaccttctc gccaacgacc gcgacccgga caccggcaac aacacggggc tgacggcctc
     5341 ggggccgacg acctcgacca acaacggcaa cgtcacggtc aacgccgacg gctcgttcag
     5401 ctataacccg gcgccgggct tcaccggcac cgacaccttc acctacacga tcaccgattc
     5461 gggcggcgcg atggacaccg gcacggtgac gctcaccgtc tcgggcctcg tctggttcat
     5521 caactccgcg gccgccgtgg gcggcgacgg gcggctcacg tcgcccttca actgctacgt
     5581 cggcgcgaac gtcccggctt ctactacctg ttactcggcg acggcggtgg acgaccccgg
     5641 cgacaacatc ttcctctaca gcggcagcta cacgggcggc gacacgctcc aaaacaacca
     5701 gaagctcatc ggccagggcg cgacgacgac gctggacgcc gtggcgggcg tcaccgtccc
     5761 cgccaacagt gacccgctgc cggcgctcaa cggcaacccg tccacggtca cgatcacgac
     5821 taccgccgcc tccaccaacg gcgtcaacct cgccgccggc gcggccaacg tcttcacgct
     5881 gcgcggcttc acggtaggca acacgacggg catcgacatc gcgtccgggg ccaactttgg
     5941 cacgctgaac gtcagcgagg tgacgctcag cggcacgggc cgcccgctga gcctcacgag
     6001 cggcgcgctg gcggcgacct ttaacagcat caccgcgacc gcggcccccg gcggacaggg
     6061 catcaacctc gcgggactga cgggcacgat ggcggtcacc ggcggcactt cgatcaccaa
     6121 cccggggacg cagtgcatcc tggtcgggac gacgacggcg agcatcaact tcgggaacac
     6181 cacctgttcg ggcggcaccg acggcgtgag cttgcagaac aactcttcgg ggacgcgcac
     6241 cttcgggacg ctctcggtct cgaacaacag cggcgcgggc ttcctccaca cgggcggcgg
     6301 cggcacgacc gtcgtcggcg ccacgaccat caccaacccg ggcacgaacg gcgtcagcgt
     6361 cgcgggctcg acgacctccg tcagcttcgg caacacgagc atcacggggt cgggcgcggc
     6421 cggggtgctc tacaccggca gcagcggcaa cctgagcttc gccgacctcg acatcacgcc
     6481 cgacaacaac gtgcaggcca tctcgaccaa cggcaacgcc ggcacgctct cggcgacgag
     6541 cggcgccatc gccacgtctg gggcggcggc cctgaacctc aactcgaccg tgctcaacct
     6601 cgtcttcgac tcggtgtcgg cggccaccaa cggcagcgcc aacaacgcca tcaacatcgt
     6661 cggcggcagc ggcgcggtct cggccccggc cggaaccatc acgggcggga cgaacccggc
     6721 cgtcatcgtc aacggcgggt cggtcagcct cgcctacggc ggcggcatct cgcagtcgaa
     6781 caacgccgct ctgctgagcg tcctgggcgg gcacacaggc acgctcacct tccagaccgg
     6841 cacgctctcg gccacaggcg gcacgggcct ccagttcgac aacgccgacg gcgcctacaa
     6901 cttcaacggc acgacgacgc tcgcgggcgg cgacgccggc gtggacatcc tcaacgactc
     6961 cggcggcgcc ttcaacttct cggccgccac gaccatcacc agcccctcgg ggacggcctt
     7021 caacgtcaac accggcgcgc ccaacgttga ctacaacggc accatcacgc agaacaccag
     7081 cggccagcgc gtggtcaacg tggacgggac gaccggcagc actgtcctct tcgacggcac
     7141 ggtgacgggc ggccagaaca gcaccggcgt ccgcatcaac aacgccaacg gcaacgtcac
     7201 gttcaccgcg acgctcacgc tcggcacgag cgtcgcgcgc atgggcagcc aagccgtgac
     7261 gattgcgggc ggatcgggcg cgaagacctt cggcgtggtc agcatcttca cgaccggcgc
     7321 atcgggcata ggcatcggcg cgacgaccag caccggcgcg atcaccacgg cgagcgggac
     7381 ggttgactcg gcgggcgcgg ctgccgtcaa catcgccggc accagcgccg cgagccgcac
     7441 gccgctcaac gtgcagctca ccaccgtcaa caccaacgcc ggcactaacg gcatcatcct
     7501 tcagaacacc tcggccacgg gcagccccgg cggcttccgc atcctcggca ccagcgccgg
     7561 catctgcggc gggcaggtga ccaacaccac gacgccttcg gtcgccgcca ccgtcacggc
     7621 ggccaacacc gccgactgca cgggcgggac gattaccggc gcgaccggcg acggcatccg
     7681 cctcaacaac gccgatcggg tctcgctcac ccgcgtgcgg gtcatcaacg gcgctcagag
     7741 cggcatcttc ggcaccgggg tcaacggctt cgagctgatc tcgtccttca tctccaacaa
     7801 cggcaacgcc gccgacgagg tcggcctcaa gttcaccgac gtcatcaacc ccggcaccgt
     7861 caacggcctc gtcggcacgg ccctcaacgg ggcgaacccg acccgcatca tcaactcgac
     7921 ggtgcggcag tcgggcgagt tcaacgtcga ggtggtcaac cgcacgggca cactcactga
     7981 cctgttcgtt gacggctgcc agttcaccga cacgaagtcg aacgccgggc cggccgacgc
     8041 cgacggcttc aacgtggaga tgcagaacac ggcggtggcc acggtgcggc tgcagaacag
     8101 cgtcttttcg aacaacttca cgcagggggc gcagctgtcg gccattgaca gcgccaacct
     8161 gacggcgacg gtgacgagca gcacgttcgc gaacaacttc gaggggttcg tctgcaacca
     8221 ctcggggaac tccgacctcg tctgcacggt gggcggcgac gtgggcacgc agggcaacca
     8281 cttcgagggt aaccccagcc tcgccgagtc cggggcgctc gtcgtgctca gcaacggcag
     8341 ctcggccacg gtgagcgcga gcctgacggg gaaggtcaag aacaaccacg tcacggcccc
     8401 ggcgaacatg gtcaaccact cgatcctcgc cttcataagc ggggtgggga cgcccagctc
     8461 gctcaacatc agcaataaca cggtcaacgg cggggcgttc gggaacccga tcctcgtgga
     8521 cacgcccgac acgggcagct ccccgaactt ctcggtcacg gcgaacaaca acgtcctatc
     8581 aggcggcagt agccacggcg tcaatgttac ggcgcggcag agctccactt cctgcgtgct
     8641 cattcagaac aacaccggca ccccgccggg cgcgtccgcc gtcgtgaggg tgcggcaggt
     8701 gagcccggcg acctacaatc tcaagcaggg ggtctcggcc tcgctcgtcc cggcgaccgt
     8761 gctgacggac aacaacccgg gggcgtcgaa ctccgtcgcc ggcacaatca acgtcgtgag
     8821 taacgccgct tgcacgacgc ctatagcgtc gttgcttggc gtccccaacc ggactttctc
     8881 ggaagtcgcc tctcgacaga cacccgcggc taccgcgaat gtgacggcac gagattccgg
     8941 cggcacacgg gaacgggagg ctctctcggc gagagcgtcc gcaattgacg cgcccgcctc
     9001 gggtgtgtcc gccggcgggt ttgcacgtgc cgactccccg gcgttctccg acgggtgggc
     9061 cgacaccttc ctgcccgccc gcaccgcttc catcaacggc ctattcgcct ctgccctccg
     9121 ctactccccg tcgaacgttg cgggtccgct cgcgagcgcg tacctcgctt ctcctgccgc
     9181 aacggctgcc ttcagcggcg agacggtcgg cccgatcaat atcggcacgc tgcccgcgac
     9241 caagagcgtc accatcaaat tccgggtcac ggttgatagc actgtgaccg ccacgaatat
     9301 ctccaaccag ggtacggtgt ccggcacgaa tttctcgccc gtgcagactg acgatccgga
     9361 cgctgctggc tcggccaacc cgaccttgac tccggtagac ctgccggacg tgaccgtggc
     9421 ggtgtcgccc gcctcggctt tggaggacgg agcagacaac ctcgtctata cgttcgcgcg
     9481 ccagggggcg acgactagca gcctgacggt gaacttcagc gtcggcggga cggccacctt
     9541 cagcaccgac tacacgcaga ccggcgcggc gaccttcacc ccgcccaccg gcacggtcgt
     9601 gattgccagc ggcagctcga cggccacagt gaccgtcgat ccgtcggctg actccacggt
     9661 cgagcctgac gagacggtga tcctgacagt cacgagcggg accgcctacg acgtcggctc
     9721 gcccagcgtg gccacaggga ctatcaccaa cgatgacacg gacgt
//
LOCUS       DASVES010000401         4899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1624407, whole genome shotgun sequence.
ACCESSION   DASVES010000401 DASVES010000000
VERSION     DASVES010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624407"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VF240_16475"
     CDS             complement(<1..391)
                     /locus_tag="VF240_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX7176859.1"
                     /translation="MTFINYASREINCKIVYYGPGLCGKTTNLQYIYDSTAPTSKGKL
                     ISLATETDRTLFFDFMPLELGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQEERMDANIESLYNLEENLQANGYD"
     gene            complement(477..959)
                     /locus_tag="VF240_16480"
     CDS             complement(477..959)
                     /locus_tag="VF240_16480"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX7176860.1"
                     /translation="MTDTPIILHEQQYEQILAVLGKLRLDSFARVVFLVDKNGQQIAV
                     QGDVGNIDTTSLASLAAGNVAATGGMARLIGEKDFPTLSHEGERESIHICIIGRVLLV
                     VVFDERSSLGLVKLRVKQAGRELALIFDEVARLSAARKAADESMFAEITDEDIDSLFN
                     "
     gene            complement(1375..1599)
                     /locus_tag="VF240_16485"
     CDS             complement(1375..1599)
                     /locus_tag="VF240_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176861.1"
                     /translation="MAAGRACLAAPLFAGFDAGRVCPAARFASDAFLAGVALRTGAFS
                     AAERFFKAGFGRAVLAAGFVLLAVDFVAVL"
     gene            1724..2269
                     /locus_tag="VF240_16490"
     CDS             1724..2269
                     /locus_tag="VF240_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7176862.1"
                     /translation="MAPQRPARPAPAPPRQPTRDEAAALKAFERAHKEFTRGRFSEAR
                     QLFRALIEQHAGVAEVTARARTYLVIAESRMRSENALPRDAEALYDRGVIELNRGDYV
                     AAQEMFERALKREPEAADIHYALAATRARLGAVEPALQSLARSLELKPSLRVRVPHDL
                     DFTLLRNEPEYERLLYPPARP"
     gene            2454..3524
                     /locus_tag="VF240_16495"
     CDS             2454..3524
                     /locus_tag="VF240_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX7176863.1"
                     /translation="MDKLQIELEHGLKLVAIGGGTGLSTLLSGLKEMVGRASEAGLWL
                     ESLSAIVTVSDDGGSSGRLRDELQMLPPGDIRNCMVALSEDSTLLARLFRHRFRGDGQ
                     LGGHSFGNLFLAALTEVTGDFVEAVRLSSEVLASKGRIYPATLNDVRLVAELEDGSRV
                     RGETNISASRSPIRRLHLEPEHCLPLPEALVAIRSADVITVGPGSLYTSILPNLLVAR
                     VAEIIGDSRAVKIFICNLMTQPGETDGYTARRHIEAVHAYAPEIRFDYVVVNDRHVST
                     EQAVAYAAEGAHQIGLTDHLLEREYGGATEIVRADLLDEGEKVRHSPQKLARVIAACY
                     EQSATLEHRHSAPAATPASVLS"
     gene            3533..>4899
                     /gene="glmU"
                     /locus_tag="VF240_16500"
     CDS             3533..>4899
                     /gene="glmU"
                     /locus_tag="VF240_16500"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX7176864.1"
                     /translation="MEKHSQPAAPQPQPPTAAGPLDVLILAAGQGTRMRSETAKVLHR
                     LGGRPIVTHVCLTAAALSPRRVFVVVGHQAEEVKRIVEEELGEGVVQFVTQAEQRGTG
                     DAVMSAREALGSGDDNSTLLVLSGDVPLVRGETLGALVHQHRTHRGRGAASTLLSVKL
                     DDPTGYGRVVRDAEGRFERIVEQKDASPEEREIREINAGIYCFETRALFSALERVQPA
                     NAQGEYYLTDVPGILRADGDDVSVFLHGDAREVSGINTRVELAEFERLLRNRTLRRLM
                     LDGVTVIDPQHTYVSTDARVGRDTVLHPGVQIEGRTRIGESCEIRAGVRITNSRVGNG
                     VTVKDYSVVVDSDIADNCSVGPFAHLRMGTRMEEQSVVGNFVEVKKSSLGRKTKAMHL
                     TYLGDATVGDETNIGAGTVTCNYDGKQKHPTVIEDNVRIGSDTMLVAPVRVGARSVTA
                     AGSVVT"
BASE COUNT          878 a         1704 c         1516 g          801 t
ORIGIN      
        1 ggtcgtagcc gttggcctgg aggttctctt cgaggttgta gagcgactcg atgttggcgt
       61 ccatgcgctc ctcctgcgag tcggcgacga agaccacgcc gtccaccccc ttgaggatga
      121 gcttgcgcga ggcgtcgtaa aagacctgcc ccggcacggt gtagaggtgg aagcgcgtct
      181 tgaaaccgcg caccgtgccc agctccagcg gcatgaagtc gaagaagagc gtgcggtcgg
      241 tctcggtcgc gagactgatg agcttgccct tcgacgtggg ggccgtcgag tcgtagatgt
      301 attgcaggtt ggtcgtcttg ccacacagcc ccggcccgta gtagacgatc ttgcagttga
      361 tctcgcgcga tgcgtaattg atgaacgtca taagaaaccc gtgataggcg ataggtgata
      421 agtgataagt taagaccgac tgctttgtct tatcacttat cactcatcac tcatcactaa
      481 ttaaagagac tgtcgatgtc ctcgtcggtg atctcggcga acatggattc gtcggccgcc
      541 ttgcgcgccg ccgacaggcg cgcgacctcg tcgaagatca gcgccagctc gcgcccggcc
      601 tgcttgaccc gaagcttcac cagcccgagg ctcgaacgct cgtcgaagac gacgacgagg
      661 agcacccgcc cgatgatgca gatgtggatg ctctcgcgct cgccctcgtg cgacagcgtc
      721 gggaagtcct tctcgccgat gagccgcgcc atcccgcccg tcgcggccac gttgccggcg
      781 gccagagacg ccagactcgt cgtgtcgatg ttgcccacgt caccttgcac ggcgatctgc
      841 tggccgttct tgtccacgag gaaaacgacc cgcgcgaacg agtccagccg cagcttgccc
      901 aacacggcca atatctgctc gtattgctgc tcgtgcaata tgataggagt gtctgtcatc
      961 agtacaggtt ctttctttcg cgggacgtgg acgttgcagg agtggcccgg agagttccgg
     1021 gagtcgaaag cgtgtggctt aaattgagga ttcgaaagga gtttactcgc ttacaaggcc
     1081 gcacgtccta tccgagcttc aaggcgatga cgggcatcgc aaccccgcca ctgactctaa
     1141 catagcgttt gaagagtcat caactgtttt tccatgttgg ccaccacgcg gggcgacttc
     1201 tgcgtggctc accgcccgca aattttgcta ctacgttgat ttatgccaca tcgcgtgtta
     1261 aactgcgccc catgttcgac agcccaaccc tggggctatc tgtgaaaacc cggtgctgcg
     1321 catgagcgac caagtcgtca agagtgctaa aaggctagcc tcccgcccgg cctcctacag
     1381 caccgcgaca aaatccacgg ccaaaagtac aaaacccgct gccaaaacgg cgcggccgaa
     1441 gccggctttg aaaaaacgct cggcggcgga gaaagctccc gtgcgcaagg cgacgccggc
     1501 caggaaggcg tcggaggcga agcgcgccgc agggcagacg cgcccggcgt cgaagccagc
     1561 aaacaagggg gccgccaaac aggcacgccc cgcggccaaa tcgcgcgccg caactcccgt
     1621 caaatcaacc aaaaaggccg cggcccctgc ggcgaagtct caaaagcaga aacaggcgcc
     1681 gtcgccttcg gcaaaatctc acacggcgaa gtcgtcgaag cctgtggccc cacagaggcc
     1741 ggcaagaccc gcgcccgcgc ctcctcgtca gccgacgcgc gatgaggccg cggcgctcaa
     1801 agccttcgag cgcgcccaca aggagttcac gcgcggccgt ttctcggagg cgcgccagct
     1861 cttccgcgcg ctcatcgaac agcacgccgg cgtggccgag gtgacggcgc gtgcgcgcac
     1921 ctatctggtc atcgccgaat cgcgcatgcg ctcggaaaac gccctgccgc gcgacgccga
     1981 ggcgctctat gaccgcggcg tcattgaact taaccgaggc gactacgtgg ccgcgcagga
     2041 gatgttcgag cgcgccctga aacgtgagcc ggaggccgcg gacattcatt acgcgctggc
     2101 ggcgacgcgc gcacgccttg gcgccgtcga gccggcgctg caatcgctcg cgcgctcgct
     2161 cgaattgaag ccttccctgc gcgtgcgcgt gccgcacgat ctggatttca cgctcctgcg
     2221 caacgagcca gagtacgagc gcctgctcta tccgcccgcc cgcccgtagt gcacgcgccg
     2281 gccactctcg tccccttcct ccccgcttcg gaaatccaac ggcccgctcc cagcgtaaat
     2341 tgtggcgctt tcgccttccc ggcgctttca aagccctttt cttttgagta tgatttcgtt
     2401 gtggcgcgcc ggagagcgcg cgcggttcgc ccgccatacg tcccggagga ttgttggata
     2461 agttacagat cgaacttgag cacggcctga agctcgtcgc catcggcggc ggcacgggtc
     2521 tctcgacgct tctgtccggc ctcaaagaga tggtcggccg agcgtcggag gcggggctgt
     2581 ggctcgaatc gctctcggcc atcgtcacgg tctcggacga cggcggaagc tccggccgcc
     2641 tgcgcgacga gttgcagatg ctcccgcccg gcgacatacg caactgcatg gtcgcgctct
     2701 ccgaggactc cacacttctg gcccgactct tccgccaccg cttccgcggc gacggccagc
     2761 tcggcggcca cagcttcggc aatctcttcc ttgccgcgct caccgaggtc acgggcgact
     2821 tcgtcgaggc cgtccgcctt tcttccgaag tcctcgccag caagggccgt atctacccgg
     2881 ccactctcaa cgacgtgcgg ctcgtcgccg aactcgaaga cggaagccgt gtgcgcggcg
     2941 agacgaacat ctcggcctcg cgctcgccca tccgccgcct acatctggag ccggagcatt
     3001 gtctgccgct ccccgaggcg ctcgtcgcca tccgctcggc cgacgtgatc accgtcggcc
     3061 ccggctcgct ctacacgagt atcctgccaa acctcctggt cgcgcgcgtg gccgagatca
     3121 tcggcgactc gcgcgccgtc aagattttca tctgcaacct gatgacgcag cccggcgaga
     3181 ccgacggcta cacggcgcgc cggcacatcg aggccgtcca cgcctacgcg cctgagatca
     3241 ggttcgacta cgtcgtcgtc aacgaccggc atgtaagcac ggaacaggcc gtggcctatg
     3301 ccgccgaggg cgcgcaccag atcgggctga ccgaccacct gctggagagg gagtacggcg
     3361 gcgcgacgga gatcgtgcgc gccgacctcc tcgacgaagg cgagaaggtg cgccacagcc
     3421 cgcagaagct cgcgcgcgtc atcgccgcct gttacgagca gtcagccacc ctcgagcatc
     3481 gccacagcgc gccggcggcg acgcccgcct ccgtcctgag ctaaggaacc gaatggagaa
     3541 gcactcccag cccgcagcac cgcagccgca gccgccgacg gccgccgggc cgctcgacgt
     3601 gctcatcctc gccgccgggc aaggcacgcg catgcgctcc gagacggcca aggttctgca
     3661 ccgcctcggc gggcgtccca tcgtcacgca cgtctgtctg acggccgccg cactgagtcc
     3721 gcggcgcgtc ttcgtcgtcg tcggccatca ggccgaggag gtcaaacgaa tcgtcgagga
     3781 ggaattgggc gagggcgtcg tgcaattcgt cacgcaggcc gagcagcgcg gcacgggcga
     3841 cgccgtgatg tccgcccgcg aggcgctcgg ctcgggcgac gacaattcga cactgctcgt
     3901 gctctcgggc gacgtgccgc tcgtccgcgg cgagaccctc ggcgcgctcg tccaccagca
     3961 ccgcacgcac cgcgggcgcg gcgcggcctc cacactcctc tccgtcaaac tcgacgaccc
     4021 aaccggctac ggccgcgtgg tgcgcgatgc cgagggccgc ttcgagcgca tcgtcgagca
     4081 gaaggacgcc agcccggagg agcgcgagat acgcgagatc aacgccggca tctactgctt
     4141 cgagacgcgc gctctcttct cggcgctgga gcgcgtgcag cccgccaacg cgcagggcga
     4201 gtattacctg acggacgtgc ccggcatcct gcgcgccgac ggcgacgacg tgagcgtctt
     4261 cctgcacggg gacgcgcgcg aggtctccgg catcaacacc cgcgtggagc tcgcagagtt
     4321 cgagcgcctg ctccgcaacc ggacgctgcg ccggctgatg ctcgacggcg tgaccgtcat
     4381 cgacccgcag cacacttacg tctccacgga cgcgcgcgtc ggccgcgaca ccgtcctcca
     4441 ccccggcgtt cagatcgagg ggcgcacgcg tatcggcgag tcgtgcgaga tccgcgccgg
     4501 cgttcgcatc accaactcgc gcgtcggcaa cggcgtcacc gtcaaggact actcggtcgt
     4561 cgtcgattcc gacatcgcgg acaactgctc ggtcggcccg ttcgcgcact tgaggatggg
     4621 cacgcggatg gaggagcagt cggtcgtcgg taacttcgtc gaggtgaaga agtcatccct
     4681 tgggcgcaag acgaaggcca tgcacctcac ctatctgggc gacgcgaccg tgggcgacga
     4741 gaccaacatc ggcgccggca ccgtcacctg caactacgac gggaaacaga agcacccgac
     4801 cgtcatcgag gacaacgtcc gcatcggctc cgatacgatg ctcgtcgcgc ccgtccgcgt
     4861 cggcgcgcgc tccgtcacgg ccgccggctc cgtcgtcac
//
LOCUS       DASVES010000402         6345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_294242, whole genome shotgun sequence.
ACCESSION   DASVES010000402 DASVES010000000
VERSION     DASVES010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294242"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..5971
                     /locus_tag="VF240_16505"
     CDS             467..5971
                     /locus_tag="VF240_16505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7176865.1"
                     /translation="MSNHARPLLLVLACCCAALSANMRFGTSAQTQSTRRVTFNTHES
                     LSLNPSVSGDGRRVVFETTAGHAGGVPGFRTVSATVFAHGFTLHELAASRAPAPAVSQ
                     DGSRVAFSSMSDPAGRNPDGNPEIFLHDGLSLTQLTDTRADDPGQRAAHGSFQPSMSD
                     DGRFIAFASNRNLAGRNADAGLEIFLYDTATHTITQLTDSTADRAAEDAKISGDGSRI
                     AFVRTRDASGDASESNSELVIMERATGAERVVALNVPGLTFTYGRAVSDDGSRVVYSA
                     RTAPNTTQVFLYDGRNQLVRQLTTLGSRSADVPLHPTISGDGSRVAFATRRAVAGFGG
                     DGGVELYLYDIPTARLSRITDAPASATAEVVSSLNDDGSLLAFNFPRVLADPSTPTEF
                     ANNSEIFLATPEPRPPFAEDLRVVNAATPRAATSTIAPDSIAIALGARLALSAREATR
                     LPDGSFPLEIGGTRVSVNGRPAQLFYAASGQVNFHVPAETETGAAQVSVHTPDGYEQR
                     GTVTVLPAAPGVFTQTGDGSGEAVALDNTTLRRSPFDVTDTGGDPRRLIIFCTGARHA
                     AGVSVSLGSAPVTVESVVPSPDLPGLDQVHVVLSTRHRRTGAATLLLNADGLAANSTR
                     LTTTSDGPPPRPALLQLSPAAALIPVGGETQFRVAAFDALGEEIPGVPIDFNSESPGI
                     ARVGPDGLASALAPGVADITARAGDTYATARLTVVERTLVVNEVLADPPDGPAGDANH
                     DGARSGADDEFVELLNGSESSLDLSGWTLRTRALGGTNETTRHRFANGETVPPGDALV
                     IFGGGDFNPHDPAFGGSPARTASTGGLGLTNSGLSVIVRDASGNLVTEFTFGRDDDPL
                     GDTANQSITRSPDVTGAFIPHTNAPGATGRRFSPGTRADGSFFLPRAGVLTAVDLQPD
                     SASIHTGQTAQFNARALDQFGRPSPGASIEFTSSDERVATVDQVTPDAAGGAAMATLS
                     GRGDGAARVIAVAREGAASVESRPADIFVTPEPPRATRVVVTPPSASINRGGEVQFTA
                     TAFDQHGRPLENVVFSWSSADAGVATVDAGGRARGMGLGDVRVNASAPDGRGGFVAGE
                     AALDVRVPLFINEVLADVPPDAASTAEVEGDANRDGIRNSDDEFVEIFNHSDTPLDIS
                     GITVSDSTSRRFTFPASTTLGALRSAIVFGGGSPPALDPAFGGSLVFTSAALGLGDAG
                     DTVSLMLNVSGVQHTIASLTFGGTGEAPAPSNQSLTRSPDSDAGHTPAVYVPHADAHA
                     ADGRVFSPGTRADGTPFGSPPPARVEVQPASMSVGVGETQSFTARAFASNGNEEFEIP
                     NVSFIWRSSDTSRLTLTPSTGAATTVTSHAGGDAVVYARAGGAEGTANIVINAPTPTP
                     TPTPSPTPSPSPTPTPSPTPLPTPTPTPEPTPSENVVINEVDSDQAGTDTAEFIELFD
                     GGAGHTPLDGLVVVLFNGGADTSYAAFDLDGFDTDAAGYFTLGSSAVSGVDRVFADGL
                     LQNGADAVALYRGSAADFPNGTAVVTASLLDALVYDTGDPDDAGLLALLNAGQTQVDE
                     DAGGGGQTNSNQRCPNGAGGRRNTFTYDQYSPTPDAQNVCMSTPTPTPTPTPTPSPTP
                     TPTPSPTPTPEPTPVPTPTPPPIPTPTPTPGPSPPPVPTAGQVIINEALVAFATSSTQ
                     ERKDFVELFNTSGQPLDISGLRITFRPAGSGNSPSTVTLPGAVGSGTTLIQARGYFLV
                     VNGAGAFGVAADFDAASPGLDFNNTTGGIKIEIDGTKLDGLSYQGAATPPASPFNDFG
                     EGSIFIFTAGTTNDLVRTPDATDTGDNASDFRRNGSSNSVTPKAVNPAPP"
     gene            complement(6048..>6345)
                     /locus_tag="VF240_16510"
     CDS             complement(6048..>6345)
                     /locus_tag="VF240_16510"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX7176866.1"
                     /translation="SGLGVQNPANLLIMREQLPEALIIVDAGVGTASDAAVAMELGAD
                     AVLMNTAIAEAQNSEQMARAMRLAIEAGRLAYLAGRMPRRLYASASSPLSGVVR"
BASE COUNT         1024 a         2484 c         1849 g          988 t
ORIGIN      
        1 gtcgagaagc tgacgatgga tgagctgcgc gaggagttta aagggccgat ctgcgagctg
       61 gcgcgcaccg gcttcgagtc gatgtcggtc gcgcgtcggg ccgccaagcg cttcgggggc
      121 gtcggcgcgc cctgccgttg cgccgaggca cgcaaggccg cctgacgccg cccgtttgcg
      181 gccgtcgcgc cacggcgcgc acccgtcgcg ccagttttct cttgcgctcg ccggcgaaag
      241 ttgtagaatg cccggcgtcg cattcgtccg aagggcgttt ttcctcatca cgctcggcac
      301 ccgctgaagc cttaacgcgc ccgactattt tcgcagcgtc gatttagttt acgtccgaag
      361 gcccaggctc gttggcccct cacgtcgcgc cgtcgcgcct gtggcagctc gtttcctcaa
      421 cgcgtcagca ctgttatgtg cgcgcacctc acacgggagc gaagccttgt ctaatcacgc
      481 acggcccctt ctgctcgtcc tcgcctgctg ctgcgcggcg ctgtccgcca acatgcgctt
      541 cggcacttcc gcacagacgc agtcaacgcg tcgtgtcacc ttcaacacgc acgaaagcct
      601 cagcctcaac ccgtccgtga gcggcgacgg ccggcgcgtc gtcttcgaga cgaccgccgg
      661 gcacgcgggc ggcgtgcccg gcttccgcac agtctccgcg accgtcttcg ctcatgggtt
      721 tactctccac gaactcgccg cctcgcgcgc ccccgcgccc gccgtctcgc aggacggctc
      781 gcgcgtcgcc ttcagcagca tgagcgaccc cgccgggcgc aaccccgacg gcaaccccga
      841 aatctttctc cacgacggcc tctccctcac acaactcacc gacacgcgcg ccgacgaccc
      901 cggccaacgc gccgcacatg gcagcttcca gccgtcgatg agcgacgacg gccgcttcat
      961 cgccttcgcc tccaatcgaa acctcgcggg ccgtaacgcc gacgccggcc tcgaaatctt
     1021 cctttacgac accgccaccc acaccatcac acaactcaca gactccacgg ccgaccgcgc
     1081 cgcggaggac gcgaagatca gcggcgacgg ctcccgcatc gccttcgtca gaacccgcga
     1141 cgcttccggc gacgcctccg aatcaaactc agaactcgtc atcatggaac gcgcgacggg
     1201 cgccgaacgg gtcgtggcct tgaatgttcc gggcctcacg ttcacttacg gccgcgcggt
     1261 cagcgacgac ggctcgcgcg tcgtctactc ggcgcggaca gcccccaaca caacgcaggt
     1321 cttcctctac gacgggcgca accagttagt gcggcagttg accaccctcg gctcgcgctc
     1381 cgcggacgtg cccctccacc cgaccatcag cggcgacggc tcccgcgtcg ccttcgccac
     1441 gcgccgcgcc gtcgcgggct tcggcggcga cggcggcgtc gagctatatc tctacgacat
     1501 ccccaccgcc cggctctcgc gtatcaccga cgcgcccgcg tccgccaccg ccgaggtcgt
     1561 ctcctcactc aacgacgacg gctcgctcct cgccttcaac ttcccacgcg tcctcgccga
     1621 cccctcgact ccaactgagt tcgccaacaa ctccgagata ttcctcgcca cgcccgaacc
     1681 gcgcccgccc ttcgcggaag acctccgcgt ggtgaacgcc gcgacgccca gagccgcgac
     1741 ttccacgatt gcgcccgact ccatcgccat cgccctcggc gcgcgtctcg ccctctccgc
     1801 acgcgaagcc acgcgcctgc ccgacggctc tttcccgctc gaaattggcg gcacccgcgt
     1861 gagcgtcaac ggccgccccg cgcaactctt ttacgccgcc tcgggacagg tcaacttcca
     1921 cgtccccgcc gagaccgaga cgggcgccgc gcaggtttcc gtccacacac ccgatggcta
     1981 tgagcaacgc ggcacggtca ctgtcctgcc cgccgcgccc ggcgtcttca cacagaccgg
     2041 agacggctcc ggtgaggcgg tcgcactcga caacaccacg ctccgccgct cgcccttcga
     2101 cgtgacagac acgggcggcg acccgcgccg cctcatcatc ttctgcaccg gagcgcgaca
     2161 cgcggccggc gtctccgtga gcctcggcag cgcacccgtc acggtcgaat cagtcgtccc
     2221 ctcgcccgat ctccccggcc tcgatcaggt tcacgtcgtc ctctccacgc ggcatcgcag
     2281 gacgggcgcg gccacgctcc ttttaaatgc cgacggcctc gccgccaact ctacaagact
     2341 cacaaccacg agcgacggcc cgccgcctcg tcccgcgctt ttgcaactat cacccgccgc
     2401 ggcactcatc cccgtcggcg gcgagacgca gtttcgcgtg gcggccttcg acgccctcgg
     2461 cgaagagata ccaggcgtgc ccatcgattt caactctgag tcgccgggca tcgcgcgtgt
     2521 cggccctgat ggactcgcga gcgcactcgc gccgggtgtg gccgacatca cggcgcgcgc
     2581 cggcgacaca tacgctaccg ctcgcctgac ggtcgtagag cgcacgctcg tcgtcaacga
     2641 agtactcgca gacccgcccg acggccccgc gggcgacgct aatcacgacg gcgcgcgcag
     2701 cggcgccgac gacgagttcg tcgaactctt aaacggctcc gagtcgtcgc tcgatctctc
     2761 cggctggacg cttcggacgc gcgcactggg cggcacgaat gaaacgaccc gccaccgctt
     2821 cgctaacggc gagaccgtcc cgcccggcga cgccctcgtc atcttcggcg gcggcgactt
     2881 caatccccac gatcccgcct tcggcggctc accggcgcgc acagcttcaa ccggcggcct
     2941 cgggctgaca aactcgggcc tctcggtcat cgtccgcgac gcctccggca atctcgtcac
     3001 cgagttcacc ttcggccgcg acgacgaccc cctcggcgac accgccaacc aatccatcac
     3061 gcgctcaccc gacgtgacgg gcgccttcat cccccacacc aacgcgcccg gcgcgacggg
     3121 ccgaagattt tcccccggca cgcgcgccga cggctccttc tttctcccgc gcgcgggcgt
     3181 gctgacggcg gtcgatctcc aaccagattc cgcgtcaatc cacacgggtc agacggcgca
     3241 gttcaacgcg cgcgccctcg accagttcgg acgcccgtcg ccgggcgcgt ccatcgagtt
     3301 cacttcgagc gacgagcgcg tggcgacggt ggatcaagtc acacctgacg cggccggcgg
     3361 cgccgccatg gcgacactgt cgggccgcgg cgacggcgcc gcgcgagtca tcgccgtcgc
     3421 gcgtgaaggg gctgcgagcg tcgagagccg gccggcggac atcttcgtca cgcctgagcc
     3481 gccgcgcgcg acacgcgtcg tcgtcacgcc tccctccgca tccatcaacc gcggcggcga
     3541 ggtgcagttc accgccacgg ccttcgacca gcacggccgt ccgttggaga acgtcgtctt
     3601 cagttggagt tctgccgatg cgggcgtcgc cacggttgat gcgggagggc gcgcgcgggg
     3661 catggggctg ggcgacgtgc gcgtcaacgc gagtgcgccg gacgggcgcg gcggctttgt
     3721 cgcgggcgag gccgcgctcg acgtgcgcgt ccccctcttc atcaacgaag tgctcgcgga
     3781 cgttccgccc gacgcggctt cgaccgccga ggttgaaggc gacgccaacc gtgacggcat
     3841 acgcaactcc gacgacgaat tcgtcgaaat cttcaaccac tccgacactc cgctcgacat
     3901 ctccggcatt accgtctccg actcgacgag ccggcgcttc accttccccg ccagcaccac
     3961 gctcggcgcg ctccgctcgg ccatcgtctt tggcggcggc tcgccgcccg cgctcgaccc
     4021 cgctttcggc ggctcgctcg tcttcacctc cgccgcgctc ggcttgggcg acgcgggcga
     4081 cacggtctcc ctcatgctca acgtctccgg cgtgcaacac acgattgcgt cgctcacctt
     4141 cggcggcacg ggagaagcgc ccgcgccctc gaaccaatcc ctgacccgct ccccagactc
     4201 cgacgccggg cacacgccgg ccgtctacgt cccgcacgcc gacgcccacg ccgccgacgg
     4261 ccgcgtcttc tcgcccggca cacgcgccga cggcacgccc ttcggctcgc cgccacccgc
     4321 ccgcgtcgaa gttcaaccgg cttcgatgtc tgtcggcgtc ggcgagactc agagtttcac
     4381 cgcccgcgct ttcgcgagca acggcaacga agagtttgaa atcccgaacg tctctttcat
     4441 ctggcgttcg agtgacacat ccagactgac gctcacacct tcgaccgggg ccgcgactac
     4501 cgtcacgtct cacgccggcg gcgacgccgt cgtctacgcc cgcgcaggcg gtgcggaggg
     4561 gaccgcaaac attgtcatca acgccccgac tccaacgccc acgccaactc cgtcgccaac
     4621 tccttcacca tcgcccacac cgacgccatc gcccacgccc ctcccaacgc cgacgccgac
     4681 ccctgagccg acgccgtcag agaatgtcgt catcaacgaa gttgactccg atcaggccgg
     4741 cacggacacg gccgagttca tcgagttgtt tgacggcggc gcagggcaca ccccgctcga
     4801 cggattggtc gtcgtcttat tcaacggcgg cgccgatact tcatatgccg ccttcgacct
     4861 cgacgggttc gacaccgacg ccgccggcta tttcacactc ggcagcagcg ccgtgagcgg
     4921 tgttgatcgc gtctttgccg acgggctttt gcagaacggg gccgatgcgg tcgcgctgta
     4981 tcgaggcagc gccgccgact ttccgaacgg cacggcggtc gtgaccgcca gcctgctcga
     5041 cgcgctcgtc tacgacactg gcgacccgga cgacgccggc ctgctcgcgt tgctcaacgc
     5101 gggtcaaacg caggtggacg aagatgccgg cggaggcggc cagaccaact ccaaccagcg
     5161 ttgtccgaac ggcgcgggcg gccggcgcaa cacatttacc tacgatcaat actcaccaac
     5221 tcccgacgcg cagaacgtct gcatgtcaac gccgacgcca acacctacac cgacgccaac
     5281 gccttctcct actccgactc caacgcccag tccgacacct acaccggaac cgacgcccgt
     5341 cccgacgcca actccgcctc ccatacccac tccgacgccg acgccggggc cttctcctcc
     5401 tcccgtcccg accgccgggc aggtcatcat caacgaggcg ctcgtggcct tcgccacctc
     5461 ctcgactcag gagcgaaaag acttcgtcga gcttttcaac acgagcggcc aaccgctcga
     5521 catcagcggc ctccgtatca ccttccggcc ggcggggtcg ggcaactcgc cgtccaccgt
     5581 cactctaccg ggcgcggtcg gcagcggcac gacactcatt caagcccgcg gctacttcct
     5641 cgtcgtgaat ggagccggcg ccttcggcgt cgcggcggat ttcgacgcgg cttcgcccgg
     5701 tctcgatttc aacaacacca cgggcgggat caaaattgaa atcgacggga caaaactcga
     5761 cggcctctcc tatcaagggg ccgcgacgcc gcccgcctcg cccttcaacg acttcggcga
     5821 aggaagcatc ttcatcttca cagccggcac gacaaacgat ctcgtccgca cgcccgacgc
     5881 gaccgacacc ggcgacaatg ccagcgactt ccgtcgcaac ggctcgtcca acagcgtcac
     5941 gccgaaggcc gtcaacccgg cgcccccata aaaaattcga gtggggagag acgaggcgct
     6001 gtctctcccc actcgaatga tgtttgatgc ccgagcgtgc cggctcttta gcggacgacg
     6061 ccggagagcg gactcgacgc gctggcgtag aggcgtctgg gcatccggcc ggcgaggtat
     6121 gcgaggcggc cggcttcgat ggcgaggcgc atggcgcggg ccatctgttc cgagttttgg
     6181 gcctcggcga tggcggtgtt catcaggacg gcgtcggctc cgagttccat ggcgacggcc
     6241 gcgtcgctgg cggtgccgac gcccgcgtcc acgatgatga gtgcttcggg caattgctcg
     6301 cgcatgatga gcaggttcgc cgggttctgc acgccgaggc cggag
//
LOCUS       DASVES010000403         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_70553, whole genome shotgun sequence.
ACCESSION   DASVES010000403 DASVES010000000
VERSION     DASVES010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70553"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VF240_16515"
     CDS             complement(<1..567)
                     /locus_tag="VF240_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176867.1"
                     /translation="MSSLLEAREVSRIYQMGSTQVAALDGVSAAVGEGEFVALLGTSG
                     SGKSTLLNLFGGLDRPSAGEIVYEGRSLAPLSKREMARYRRRAVGMIFQNFNLIPAMT
                     AAENVELALAFGKVRGRERAERARELLGRVGLGERAAHRPSELSGGEQQRVAIARALA
                     NRPRVLLADEPTGNLDSTRAHELLALLRG"
     gene            complement(730..1092)
                     /gene="trxA"
                     /locus_tag="VF240_16520"
     CDS             complement(730..1092)
                     /gene="trxA"
                     /locus_tag="VF240_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7176868.1"
                     /translation="MAEHVVEVRDETFESDVLRHERPVIVDFGAPWCPPCRALEPTFE
                     ALAGEFDGAVRFLKLNIDDNPETTQRYGVRGIPTLILFREGREVERIVGAPARKVIAR
                     MIEKHAGESEGVARASSS"
     gene            1350..2018
                     /locus_tag="VF240_16525"
     CDS             1350..2018
                     /locus_tag="VF240_16525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7176869.1"
                     /translation="MNNSDTNSARHGGADLFRDLSAEELAEIERISVTRALPRGFVLY
                     TPGETGEALFLLRGGSVQIYRLSPEGRKLTIAHLLPLSFFGEMSCIGQGMYDAYAETT
                     DDSEVMALNCADLERLLATKPQIARRILEAFGRRVLEAEQQLEDIAFKGLVARVASLL
                     LREAEGDEVTGMSHQDIAERLGVYRETATNALNDLKAAQIINTGRKRISIIDRERLRR
                     VAGA"
BASE COUNT          348 a          724 c          681 g          331 t
ORIGIN      
        1 gccgcgcagg agagcgagga gttcgtgcgc gcgcgtcgag tcgagattgc ccgtcggctc
       61 gtcggcgagg agaacgcgcg ggcggttggc gagggcgcgg gcgatagcga cccgctgctg
      121 ctcgcccccc gatagctccg acgggcggtg cgcggcgcgc tcgccaagtc cgacgcggcc
      181 caacaactcg cgcgcccgct ccgcgcgctc gcgccccctg accttgccga aggcgagggc
      241 cagctcgacg ttctcggcgg cggtcatcgc cgggatgagg ttgaagttct gaaagatcat
      301 tccgacggcg cggcgtcgat agcgggccat ctcgcgcttc gagagcgggg cgagcgagcg
      361 tccttcgtag acgatctcgc cggccgacgg gcggtcgaga ccgccgaaga gattcagcag
      421 cgtcgatttc cccgaccccg aggtgccgag caaagcgacg aactccccct cgccgaccgc
      481 cgccgacacg ccgtcgagcg cggccacctg cgtgctcccc atctggtaga ttcttgagac
      541 ctcgcgcgcc tctaaaaggc tggacatggc gattcaccct cgaacgcggc ccgtgccggt
      601 ggcgaagatt accatgaaag cgtgcgggcg tgcgtaccct tgtgcggggc cgtgtggcgt
      661 gtcaaaagga aagacggcgt gcgccgaaat acgacgcacg ccgtctgact ttcccagcct
      721 tgattgtctc tacgacgaac tcgcgcgcgc gacgccttca gactcaccgg cgtgcttctc
      781 gatcatgcgc gcgatgacct tgcgggcggg cgcgccgacg attcgctcca cctccctgcc
      841 ctctcggaag aggatgaggg tcgggatgcc cctgacgccg taacgctgcg tcgtctcggg
      901 gttgtcgtcg atatttagct tcaggaagcg caccgcgccg tcgaactcgc cggccagcgc
      961 ctcgaacgtc ggctcaagcg cccggcaggg cgggcaccac ggcgccccga agtccacgat
     1021 caccggccgc tcgtgccgca gcacgtcgct ctcgaaggtc tcgtctctca cttccacaac
     1081 gtgttccgcc attgttcgtt cacctctttc ggcgccggcc gcgtggccgc aaatccaacc
     1141 cggccggtcg cctttgtttt gcgcgacgat tatgcccgcg acagcgcgcc gccgtcagta
     1201 atctcgatta caaagcgcgc gcctccgctt ctgtaataat gcgcggggcc gcgcccgagc
     1261 gcgcacgcgc cgtggtcaaa gcgcgcgcgc ggacgtagaa ttttccatgt caggcgcgtt
     1321 gccccacggg cgtcaggatg aatcgagaga tgaacaattc agacacaaac tctgcgcgac
     1381 acggcggggc cgacctcttc cgcgacctca gcgccgagga gctggcggag atcgagcgca
     1441 tcagcgtcac gcgcgctctg ccccgcggct tcgtgctcta cacgccgggc gagacgggcg
     1501 aggcgctctt tctcctgcgc ggcgggtcgg ttcagattta ccgtctctcg cccgagggcc
     1561 ggaagctcac catcgcgcac ctgctgccgc tttccttctt cggcgagatg agctgcatcg
     1621 gtcagggcat gtacgacgcc tacgccgaga cgaccgacga ctccgaggtc atggccctta
     1681 actgcgccga cctcgaacgc ctgctggcga cgaagccgca gatcgcgcgc cgcatcctcg
     1741 aagccttcgg ccgccgcgtg ctcgaagccg agcagcagtt ggaagacatc gccttcaagg
     1801 gactcgtcgc gcgcgtcgcc tcgctcctgc tccgcgaggc ggagggcgac gaggtgacgg
     1861 ggatgtcgca tcaggacatc gccgaacgcc tcggcgtcta ccgcgagacg gccaccaacg
     1921 cgctcaacga cctgaaggcc gcccagatca tcaacaccgg caggaaacga atttccatca
     1981 tcgaccggga gcgcctgcgg cgcgtcgcag gcgcgtgagt ggtggtaagt aacagggtac
     2041 tcccggcaga aaggcgcggc ggccggggcg gaatgaagcc gccc
//
LOCUS       DASVES010000404         9915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_459786, whole genome shotgun sequence.
ACCESSION   DASVES010000404 DASVES010000000
VERSION     DASVES010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459786"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VF240_16530"
     CDS             <1..217
                     /locus_tag="VF240_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176870.1"
                     /translation="QGLTELGGTVRVIDERQRQADERQRQSDERQREADERNRETDER
                     LSALINTVERYISERRNGKSDNGKSDN"
     gene            324..1331
                     /gene="pta"
                     /locus_tag="VF240_16535"
     CDS             324..1331
                     /gene="pta"
                     /locus_tag="VF240_16535"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX7176871.1"
                     /translation="MNILEQIRQRAAADPQHIVLPEGEDPRTAAAAAACARDRLARVT
                     VLGGEEKIRAAAREAGADLSGVEIIDHRRAADFEKVAAYYHELRRAKGLMPDEARIAL
                     QDPLYYGNLMVRAGRADGSVAGATNTTAHTVRAALQCIGVREGYKLVSSFFVMALPDA
                     RFGADGALIYADCGVVIEPSAAELAEIAIASAESCRALLGVEPRVAMLSFSTKGSAKH
                     KLIDKVVEATKTVRARRPDLEIDGELQADAALVPAVGESKAPGSGVAGRANVLVFPDL
                     QAGNIAYKLTERLAGATAIGPILQGLDRPANDLSRGCKAEDIVDAVAITAVQAQARKQ
                     G"
     gene            1524..2711
                     /locus_tag="VF240_16540"
     CDS             1524..2711
                     /locus_tag="VF240_16540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7176872.1"
                     /translation="MIPRLKFLSLFFAAVFILPAATAHAQSGVRPKPTPADDGDERVF
                     TEEVRIPVFATDERGRFDPALEIPDVLVVEDDVPQQVKSVRRIPASVLLLLATGGELN
                     PSMRVSTTREVALNVVENLRDGDRVAIVEFDSRAQVAQGWTADREEAAHALRSKLAFG
                     RGSRLSQAIIRAAQLFEGEPAGNRHLVVVTDGVEAPARIDAKDALLVLGVDTPESKAQ
                     AAEATRRLIDAQATVHVVSYAALGRKAMKERERPKEVAGMAQSRQDIAVAGINPTQPI
                     GMRGPGINGPSVNSGLRIDPTMKRVHKAYERAMKKGEDRLKALTEETAGRLIQPATEE
                     ELIERGAEVAREIGAQYVVTYRPKRSLGSSPATEYRRIRVHARRIGLNVRARRGYVVG
                     AMR"
     gene            complement(2808..3959)
                     /locus_tag="VF240_16545"
     CDS             complement(2808..3959)
                     /locus_tag="VF240_16545"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7176873.1"
                     /translation="MPEVQESRAHVLIADDEPEIRSVLLELLGAGYECAAVSSAEEAL
                     ELLREQSFDLVISDIMMGGMSGLEMVPHVLRLRPETAVVMLSGVQTVESAVGAMRAGA
                     FDYVMKPFDLLHVEAAVRRALEHKCLRDAKRRYENQLEEMVAQRTAELDRTLHSLEDA
                     YRSTLKALTSALEARDHETRGHSERVVSFSLRLGRELGLAPTHLRSLEFGSLLHDIGK
                     IGVPDAILRKPAALDEEEWARMREHPLHGQKILEGVEFLEGASRVVAQHHEKWDGSGY
                     PLGLRGMEIDITARIFSVADAFDAMTSDRVYRRGRPYEAAAAELEEYAGRQFDPEVVR
                     AFHRVPPEDWEEMRARSLEALRERAASAQRRGVAAARDAAAPAVSLRAG"
     gene            4198..4281
                     /locus_tag="VF240_16550"
     tRNA            4198..4281
                     /locus_tag="VF240_16550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4232..4234,aa:Leu,seq:caa)
     gene            complement(4305..4703)
                     /locus_tag="VF240_16555"
     CDS             complement(4305..4703)
                     /locus_tag="VF240_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176874.1"
                     /translation="MTRKILSLVLVALLMNLVAVPHAYAGTKEEKQARLAAKVKANIL
                     KLGTGEASRVKVVLRDKTKMEGYVSAAGDETFTVTDPKTGAATAVAYPQVRSVKGNNL
                     STGAKIAIGASVAAAVIFLILWKVIVDEDE"
     gene            5145..5684
                     /gene="msrB"
                     /locus_tag="VF240_16560"
     CDS             5145..5684
                     /gene="msrB"
                     /locus_tag="VF240_16560"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7176875.1"
                     /translation="MNRRLFLRSGIVMGAAALLSKSGLASALSRGGALNAGQEVAALR
                     KVVKSDAEWKRILTPDQFNVTRRKGTEAPYTSPLNKVHAKGVFACVCCDLPLFSSDTK
                     FESGTGWPSFWAPIAKQNVREEVDDSMSERRTEVLCARCDAHLGHVFDDGPKPTGLRY
                     CMNGLALKFVRSGGARRRA"
     gene            5703..6104
                     /gene="msrB"
                     /locus_tag="VF240_16565"
     CDS             5703..6104
                     /gene="msrB"
                     /locus_tag="VF240_16565"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7176876.1"
                     /translation="MNKKITKTEEEWRSELTPEQYAVARQKGTERPFTGEYHDSKERG
                     TYKCAACGNELFTSDAKFDSGCGWPSFTAPTAADNVRTEVDTSHGMRRTEVLCASCDS
                     HLGHVFEDGPAPTGLRYCINSVILDLEKEEA"
     gene            6166..7581
                     /locus_tag="VF240_16570"
     CDS             6166..7581
                     /locus_tag="VF240_16570"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX7176877.1"
                     /translation="MLSNGRPRALRRDGRLLRVGALALLVLLVVNCSGEQDTEAQRRR
                     QQQPDRNAAAQSPTPRAPGGVLTGKDALGDWTTDAPGVRRRITTADLPRPFETRSVDN
                     GPRLVARPQDAWPKAPAGFKVEEFAAGLEDPRLIRAAPNGDLFVAESRAGKIRVLRPS
                     AGAGKPEANEVFVSGLDRPFGIAFYPPGPDPQYVYVANTGAVVRFPYQSGDLRARGPV
                     ETIVPDIPGGGLLRGGGHWTRDVVFSRDGRKMFVSVGSQSNVWEGERANEERRANILE
                     YNPEGTGERVYAWGIRNAVGLAVNPQTGELWASVNERDGLGDDLVSDYITRVREGGFY
                     GWPWFYLGPNQDPRHAGKRADLRDKVIVPDVLIQSHSASLQMEFYTGAQFPAEYRGDA
                     FASEHGSWNRARRTGYKVIRVPVRDGAPTGEYEDFMTGFVTPEGDVWGRPVGVATGRD
                     GALFVTDDGSKTVWRVSYQGG"
     gene            7854..9779
                     /locus_tag="VF240_16575"
     CDS             7854..9779
                     /locus_tag="VF240_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7176878.1"
                     /translation="MFVSINARLLFDALRILSLSFAFILLFCIASSEASAAPRWSAKL
                     DGRVVFYQATELGVLIAGTEKSLYAIEAETGEVLWRRKDARLEETDVAAVPGTDVLLL
                     SFERGDKTRLEAVDLLTGGALWRTERVRGSVMQMAFEPNTGLLGAVFVRDARGRAKEG
                     FKRKPTVRVFDLREGREVWKRDLESEVEMMPALVGGDGEVPYTLDNYRAPAFLDGRLY
                     LFYEGVTSLDARNGEERRREKFRVNEEGLALTEADPVADERHVYFSGRGRVRAVARAD
                     GREAWESKDLGLTPEIALVGGALFVRTGGQFTSLEDGEAVARGPYGVSAIDSATGKTL
                     WRYKGADKGITNMVIPNRSTVVVADRDDLIVIDATTGKRRSKLAHGIERAAFVLLNER
                     GQTVVGGRNEIAAFDLAAGREVWRERHTPPGRGIFRTVAAVAARAASLYFRYGGAVST
                     AFGGYRLLSTAGSLRFSGLAAHATVPELTSLATDYSRELLRERFSPYGILSRARGAVG
                     TARDAAGVLRDLPSVRERLATTRPSINVPRPSADDVQERLLDRLDPASQLERLSRFLW
                     RRQRLAALRGQWMYFYTDLRGEGHGLVGVNVNTGAPERAVRLGDPDQRFISDEAAGLL
                     YSSKDNRLNAFPLGTRD"
BASE COUNT         1705 a         3390 c         3231 g         1589 t
ORIGIN      
        1 ccaagggctg acggagttgg gcgggaccgt gcgcgtcatc gacgaacggc agcgtcaggc
       61 cgacgagcgt cagcgacagt ccgacgagcg gcaacgtgaa gcagacgagc ggaaccgcga
      121 gacggacgag cggctgtccg cactcatcaa caccgtggag cgttacatca gcgagaggcg
      181 caacggcaag tcagacaacg gtaagtcaga caactaagcc gcgcccggcc gcgcgggcga
      241 atgaccgcgc gccgataatt ttagacgagc gaccgaaaca gcgtcgcgtc tcattcatcg
      301 ccccgacctc gactccatac tccatgaaca ttctcgaaca gatacgacag cgcgccgccg
      361 ccgacccgca gcacatcgtc ctgcccgagg gcgaagaccc gcgcacggcg gccgcggcgg
      421 ccgcgtgcgc gcgcgaccgc ctggcacgcg tcaccgtgct cggcggcgag gagaagattc
      481 gcgccgcggc gcgcgaggcg ggcgcagacc tctcgggcgt cgagatcatc gatcaccgcc
      541 gcgcggccga cttcgagaaa gtggccgcct actatcacga gctgcgccgg gcgaagggac
      601 tgatgcccga cgaggcgcgc atcgccctcc aagacccgct ctactacggc aacctcatgg
      661 tgcgcgcggg ccgcgccgac ggctccgtgg ccggcgcgac caacaccacg gcccacaccg
      721 tccgcgccgc gctccagtgc atcggcgtgc gcgaagggta caagctcgtc tccagcttct
      781 tcgtcatggc cctgcccgac gcgcgcttcg gcgccgacgg cgcgcttatc tacgccgact
      841 gcggcgtggt catcgaacct tcagccgccg aactcgccga gatcgccatc gcttcggccg
      901 agagttgccg cgcgctgctc ggggtcgagc cgcgcgtcgc catgctctcg ttctcgacga
      961 aggggagcgc caaacataag ttgatcgaca aggtggtcga ggccacgaag accgtgcgcg
     1021 cgcgccggcc cgatttggag atcgacggcg agttgcaggc cgacgcggcg ctcgtccccg
     1081 cggtcggaga atcaaaggcg ccggggtcag gtgtcgcggg ccgcgccaac gtcctcgtct
     1141 tccccgacct acaggccggc aacatcgcct acaagctcac cgagcgcctc gccggcgcca
     1201 cggccatcgg ccccatcctg caaggactcg accgccccgc caacgacctc tcgcgcggct
     1261 gcaaggccga agacatcgtt gacgccgtcg ccatcacggc cgtccaggcg caagcacgga
     1321 aacagggatg aggaagagca gtaagcagta agcagtgagc agtgagcagt gagcagtcaa
     1381 taccagccca gcgtcctgtg tcctgactgt ttactgctta ctgctcactg ctcactccct
     1441 ttcatccttg cttctggcga accgcgcggc ctccggccgg catcagagtc ttgtaagcaa
     1501 catcccgacg gcggagttaa gccatgatcc cgcgcctcaa gttcctgtca cttttcttcg
     1561 ctgccgtctt tattttgccc gccgccacag cgcacgcgca gagcggagtc cgcccgaagc
     1621 cgacgccggc cgacgacggt gatgagcgcg tcttcacgga ggaggtacgc atccccgtct
     1681 ttgcgaccga cgagcgcggg cgattcgacc cggcgctcga aataccggac gtgctcgtcg
     1741 tcgaggacga cgtgccgcaa caggtcaaga gcgttcgccg catcccggcg agcgtgcttc
     1801 tgctcctcgc cacgggcggc gagctgaacc cgtcgatgcg cgtctcgacg acgcgtgagg
     1861 tcgccttgaa cgtcgtggag aatctgcgcg acggcgaccg cgtcgccatc gtcgagttcg
     1921 acagccgggc gcaggtcgcg caaggttgga cggccgaccg cgaggaggcg gcgcacgcgc
     1981 ttcgcagcaa gctcgccttc gggcgcgggt cgcgtctgtc gcaggccatc attcgcgcgg
     2041 cgcaactctt cgagggcgag ccggcgggca accgccacct ggtcgtcgtc accgacggcg
     2101 tcgaggcccc ggcgcgcatc gacgctaaag acgcgctgct cgtcctcggc gtggacacgc
     2161 ccgagtcgaa ggcgcaggcc gccgaggcga cgcgccggct catcgacgcg caggccaccg
     2221 tccacgtcgt cagctacgcc gcgttggggc ggaaggcaat gaaggagcgg gagcggccca
     2281 aggaggtggc cggcatggcg cagagtcggc aggacatcgc cgtcgccggc atcaacccca
     2341 cgcagcccat cggcatgcgc ggccccggca tcaacggccc ctccgtcaac tcggggctgc
     2401 gtatcgaccc gacgatgaag cgcgtccaca aggcatacga gcgcgcgatg aagaagggcg
     2461 aggatcgtct gaaggcgctg accgaggaga cggccggtcg cctgattcag ccggcgaccg
     2521 aggaggagtt gatcgagcgg ggcgcggagg tcgcccgaga gatcggcgcg caatacgtcg
     2581 tcacctaccg ccccaagcgc tcgctcggca gctcgcccgc caccgagtat cggcgcatac
     2641 gcgtccacgc gcgccgcatc ggcctcaacg tgcgcgcccg ccgcggctac gtcgtcggcg
     2701 cgatgcgata agccgaaagg ggtaaagggt aaaggggaag gggtaaggga aagcaagaca
     2761 gcttctccct taccccttcc cctttaccct ttaccccttc cagttcttca gccggcgcgc
     2821 agcgagacgg cgggcgcggc tgcgtcgcgc gccgcggcga ctcctctgcg ttgcgccgac
     2881 gcggccctct cccgcagcgc ctccagagag cgtgcgcgca tctcctccca gtcttccggc
     2941 ggcacgcgat ggaaggccct gacgacctcc gggtcgaact gccgccccgc gtactcctcg
     3001 agttcggcgg ccgccgcctc gtagggccgt ccgcgccggt agacgcggtc gctcgtcatc
     3061 gcgtcgaagg cgtcggccac cgagaagatg cgcgcggtga tgtcgatctc cataccacgc
     3121 aggcccagcg ggtagcccga gccgtcccac ttctcgtggt gctgcgcgac gacgcgcgac
     3181 gcgccttcca gaaactccac gccctctaaa atcttctggc cgtgcagcgg gtgctcgcgc
     3241 atccgcgccc actcctcctc gtcgagcgcg gccggcttgc gcaggatggc gtcgggcacc
     3301 ccgatcttgc cgatgtcgtg caggagcgag ccgaactcca gcgagcgcag atgcgtcggg
     3361 gccagcccca actcgcgccc gagcctgagc gagaagctga cgacgcgctc ggagtggccg
     3421 cgcgtctcgt ggtcgcgcgc ttcaagagcg gaggtgagcg ccttcaaagt cgagcggtag
     3481 gcgtcttcga gcgagtgcaa cgtccggtcg agttccgcgg tgcgttgcgc caccatctct
     3541 tcgagttgat tctcgtagcg gcgcttcgcg tcgcgcagac acttgtgctc cagcgcccgg
     3601 cgcaccgccg cctcgacgtg gagcaggtcg aacggcttca tcacgtagtc gaacgccccg
     3661 gcgcgcatcg ccccgacggc gctctcgacc gtctgcacgc cgctcagcat gacgacggcc
     3721 gtctcgggcc gcaggcgcaa gacgtgcggc accatctcca gccccgacat cccgcccatc
     3781 ataatgtcgc tgatgacgag gtcgaacgat tgctcccgca acagctccag cgcctcctcg
     3841 gccgagctga cggcggcgca ctcgtagccg gcccccagca actccaacag gacgctgcgg
     3901 atttccggct cgtcgtccgc gatcagcacg tgcgcgcgcg actcctgcac ttcaggcatc
     3961 gatatagact ccgggatatt gagagagtgt tgtttaagaa cgtgagggac aaaaagacgg
     4021 cgccggtcgc tacagaaggc cgaccggccg ccgcctgagg caaaaccatc ttaccccctt
     4081 acaccccctt cagacaaccc tctttgtcag gggatgcaac ttttttgcat gcctcgatac
     4141 ctcgctgccc acatttgacg acccggcggc ccaacgccta tcatgcaacc cgccagcgcc
     4201 ggagtgatga aattggtata catagcagac tcaaaatctg ccgtccgcaa gggcatgggg
     4261 gttcgactcc cccctccggc atcatcaaca acttacgcgc atcctcactc gtcctcatcg
     4321 acgatgactt tccacaggat gaggaagatc acggcggcgg cgacgctggc gccgatggcg
     4381 atcttggcgc ccgtcgagag gttgttaccc ttgacgctcc tgacctgcgg ataggcaacg
     4441 gccgtcgccg cccccgtctt cgggtcggtg acggtgaagg tctcatctcc ggcggcgctc
     4501 acgtaacctt ccatcttcgt cttgtcccga agcaccacct tcacacgcga cgcctccccg
     4561 gtgcccagct tgaggatgtt cgccttcacc ttcgcggcga ggcgggcctg cttttcttcc
     4621 ttcgtgccgg cgtaagcgtg cggcacggcg accaagttca tgagcaacgc gacgagcacg
     4681 agcgacagga tttttctggt catcacttcc tccgttgtgg aattggaact gcggcaatac
     4741 tgcgagagca aatagcgcgc cccagcatgg ggcttgaatc gacgtatcgg gactcagaaa
     4801 tcggcgcgct cggagtgccg gcgccacacc tgaaagataa ttatggcgtt ggttcgatcc
     4861 ttcgcgcccc ctccgcgtcc tttgcgcctc ttgcggtgaa tctacttccc cttcactcaa
     4921 gtgaacatcg ctctcatgcc acagcttttg taaggcccgg aggcaaaccg cggagaggcg
     4981 taaccgcgta tatttaacgc tcgcgtctta cgccgacagg agtcgcggcc gagggttgat
     5041 gcttatcggg ggcgcggccg gtgttgacct tacggctcat gtctcggctg caacgctgct
     5101 gaagtgccgg cgcatcgaag atgacatatt aaaggagtat gggaatgaac aggcgtcttt
     5161 ttctgaggtc gggtattgtc atgggggcgg cggccctgtt atcaaagtcg ggcctcgcgt
     5221 ccgccctgag tcgcggcggc gctttgaacg cggggcagga ggtggccgcg ctcaggaagg
     5281 tggttaaatc ggacgcggag tggaaacgga ttctgacgcc cgaccagttc aacgtgacgc
     5341 ggcgaaaagg caccgaggcg ccctacacca gcccgctcaa caaggttcac gccaaaggcg
     5401 tcttcgcctg cgtctgctgc gatctgccgc tgttcagctc ggacacgaaa ttcgagtccg
     5461 gcacgggctg gccaagtttt tgggctccaa tcgccaaaca gaacgtgcgc gaagaagtgg
     5521 acgacagcat gagcgagagg cggacggaag tgttatgcgc gaggtgtgac gcgcacctcg
     5581 ggcacgtctt cgacgacggc cccaaaccca cggggctgcg ctactgtatg aacgggctgg
     5641 cgctcaagtt tgtgagaagc ggcggcgcgc gccggcgggc gtaaagcaga gggaggccga
     5701 aattgaataa gaagatcacg aagaccgaag aggagtggcg gagcgaactc accccggagc
     5761 agtatgcggt ggcccgtcag aaagggacgg agcggccctt caccggcgag tatcacgact
     5821 cgaaggagcg gggcacatac aagtgtgcgg cctgcggcaa cgagctgttc acctccgacg
     5881 cgaagttcga ctccggctgc ggctggccga gtttcactgc cccgacggcc gccgacaacg
     5941 tccgcaccga ggttgacacc agtcacggca tgcgccgcac ggaagtcctg tgcgcgagtt
     6001 gcgactcaca cctcgggcac gtctttgaag acggccccgc gcccacgggg ctgcgctact
     6061 gcatcaactc cgtcatcctc gatctggaaa aagaagaggc gtgaccctcg gccccggaca
     6121 tgaatcgctc cccctcgtac cagcaattcg tggagtgaat ttatcatgtt gagtaacggg
     6181 cgcccgcgcg cgctccggcg tgacggccga cttttgagag tcggcgcact cgcacttctc
     6241 gtcctcctcg tcgtcaactg ctccggcgag caggacacgg aggcgcagcg gcggcggcag
     6301 cagcaacccg acaggaacgc cgccgcgcag agtcccacgc cgcgcgcgcc gggcggcgtg
     6361 ctcacaggca aagacgctct gggcgattgg acgacggacg cgccgggcgt gcgccggcgc
     6421 atcaccacgg ccgatttgcc ccgccctttc gagacgcgct ccgtggacaa cgggccgcgt
     6481 ctcgtcgcgc gaccacagga cgcgtggccg aaggcgccgg ccgggttcaa ggtcgaggag
     6541 ttcgccgccg ggcttgaaga cccgcgtctg attcgcgcgg cgccgaacgg cgacctcttt
     6601 gtcgccgaaa gtcgcgcggg gaagattcgc gtcctgcgcc cgtctgcggg cgcaggaaag
     6661 cccgaggcga acgaagtttt tgtttccggc ctcgaccgcc cgttcggcat cgccttctac
     6721 ccgcccggcc ctgacccgca atacgtctac gtcgccaaca ccggcgcggt cgtgcgtttc
     6781 ccctaccaga gcggcgacct ccgcgcgcgc ggccccgtgg agacaatcgt gcccgacatc
     6841 cccggcggcg ggcttttgcg cggcggcggc cactggacgc gcgacgtggt cttctccaga
     6901 gatggccgca agatgttcgt ctccgtcggc tcacaatcaa acgtctggga gggcgagcgc
     6961 gccaacgagg agcgacgcgc caacatcctt gagtacaacc ccgaagggac gggcgagcgc
     7021 gtctacgcgt ggggcatccg caacgcggtg gggctagccg tcaacccgca gaccggcgaa
     7081 ctgtgggcgt cggtgaacga gcgcgacggc ctcggcgacg acctcgtctc cgactacatc
     7141 acgcgcgttc gcgagggcgg cttctacggc tggccgtggt tctacctcgg cccgaatcaa
     7201 gacccgcgac acgcgggcaa gcgcgccgac ttgcgcgaca aggtgatcgt ccccgacgtg
     7261 ctcatccagt cgcactcggc ctctctccag atggagttct acaccggcgc gcagttcccc
     7321 gccgagtacc gcggcgacgc cttcgcctcc gagcacggct catggaaccg cgcgcgccgc
     7381 acgggctaca aggtgattcg cgtgcccgtg cgcgacggcg cgccgacggg cgagtatgag
     7441 gatttcatga ccgggttcgt cacgcccgag ggcgacgtgt gggggcggcc cgtcggcgtc
     7501 gccacgggcc gcgacggcgc gctgttcgtg acggacgacg gctcgaagac cgtttggcga
     7561 gtttcgtatc aaggcgggtg acgatgagcc accgtaggtg gcggcagaat gtagccacgg
     7621 gcgctgggcc cgtggtagca gcccggaata attaacagag ccccggaggg gcggaagaac
     7681 agcgaccggc atcgtgctca acgtcgagat tcttccgccc ctccggggct tcaatatttg
     7741 ttgtcgcatc ttacccacgg cttacgccgt gggctacatt cttccgccgc ctccggcggc
     7801 tcgcgtcgtc tcggttcttt cgcagcaagc tcccggcgag gtaaactgtc ctcgtgtttg
     7861 tctcaatcaa cgctcgcctg ctcttcgacg cgttaagaat cctctccctg tctttcgcgt
     7921 tcatcctttt gttctgcatc gcctcttcgg aggcgtcggc ggcgccgcgc tggtctgcca
     7981 agctcgacgg tcgcgtggtg ttctatcagg cgacggagct gggcgtgctc atcgccggga
     8041 cggagaagtc tctgtacgcc atcgaggcgg agacgggcga ggtgttgtgg cgtcggaagg
     8101 acgcgcgcct cgaagagacg gacgtggcgg ccgtccccgg cacggacgta ctccttttga
     8161 gttttgagcg cggcgataag acgcgtctgg aggcggtcga tctgctgacg ggcggcgcgc
     8221 tgtggcgcac ggagcgtgtg cgcggctccg tgatgcagat ggcgtttgaa ccgaacacgg
     8281 ggctgctcgg cgccgtcttc gtgcgcgacg cgcgcgggcg tgcgaaggag ggtttcaaac
     8341 gcaagccgac ggtgcgtgtg ttcgatctgc gggaggggcg cgaggtctgg aagcgcgatc
     8401 tcgaaagcga ggtcgagatg atgcccgcgc tcgtcggcgg cgacggcgag gtgccctaca
     8461 cgctcgacaa ctatcgcgcg ccggccttcc tcgacggccg tctctatctc ttctacgagg
     8521 gcgtcacctc tctcgacgcg cgcaacggcg aggagcgccg gcgcgagaag tttcgcgtca
     8581 acgaggaggg gctggcgctc accgaggccg acccggtcgc cgacgagcgc catgtctatt
     8641 tctccggccg gggccgcgtg cgcgccgtcg cgcgcgccga cgggcgcgag gcgtgggagt
     8701 cgaaggattt ggggctgacg ccggagatcg cgctcgtggg cggcgcgctc ttcgtgcgga
     8761 cgggcgggca gttcacaagc ctcgaagacg gcgaggcggt ggcgcgcggc ccctacggcg
     8821 tgagcgccat cgactccgcg acgggcaaga ccctctggcg ctacaagggc gccgataagg
     8881 gcatcacgaa tatggtcatc cccaaccgct cgaccgtcgt cgtcgccgac cgcgacgacc
     8941 tcatcgtcat cgacgcgacg acgggtaagc gccgctcaaa gctcgcgcac ggcatcgagc
     9001 gcgccgcctt cgtgctctta aacgagcgcg gccagacggt ggtcggcggc cggaacgaaa
     9061 tcgccgcctt cgacctcgcc gctgggcgcg aagtctggcg cgagcgccac acgccgcccg
     9121 gtcgcggtat cttccgcacg gtcgcggcgg tcgcggcgcg cgccgcctcg ctctacttcc
     9181 gctacggcgg cgccgtctcc acggccttcg gcggctatcg cctcctgagc acggccggga
     9241 gcctgcgctt ctccggtctc gccgcgcacg ccacagtccc cgaactcacg agcctcgcca
     9301 ccgactactc gcgcgaactc ctgcgcgagc gcttctcgcc ctatggcatc ctctcgcgag
     9361 cgcgcggcgc tgtcggcacc gcgcgcgacg ctgcgggcgt gttgcgtgac ctgccctcgg
     9421 tgcgcgagcg cctagcgacg acgcggccgt ccatcaacgt gccgcgccct tcggccgacg
     9481 acgtgcagga gcgcctgctc gaccgtctcg accccgcctc gcagctcgaa cgcctctcgc
     9541 gcttcctctg gcgccggcag cgtctcgccg ccctgcgcgg gcaatggatg tacttctaca
     9601 ctgacttgcg cggcgagggc cacggcctcg tcggcgtcaa cgtcaacacg ggcgcgcccg
     9661 agcgcgccgt ccgcctcggc gaccccgacc aaaggttcat cagcgacgag gccgccggcc
     9721 tcctctattc ctcgaaagac aaccgcctca acgcctttcc gctcggcacg cgcgactgac
     9781 gaattcgcac ccacgtcaaa aagagttcgt ttcaaaacct caacgcgaat gatgtctgta
     9841 ggggcagggc caagtccgcg gcccgctgct acggaggaat taacatggat ggacaggatg
     9901 tgcaggatga agaac
//
LOCUS       DASVES010000405         7078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1626248, whole genome shotgun sequence.
ACCESSION   DASVES010000405 DASVES010000000
VERSION     DASVES010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626248"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VF240_16580"
     CDS             complement(<1..1493)
                     /locus_tag="VF240_16580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7176879.1"
                     /translation="MRPSSRKDFLFRGLAPALLALQLVAAPAPAAQQPAAATATTQKP
                     ATATLTAAERAAIERVKLETVREVVTRLSSKEMEGRGTAQAGGERAAKYLADKFAAFG
                     LKPLGDAGTYLQAVKFKATEVQSETFLKAGDATLKHGSDFILPPPFTYEQADVTAPVV
                     FIGYGVTSTELKRDDLAGLDLKGKIVVMLSGRPRGVDEAAWAKASSPQAKVVGLFGRG
                     IGGLLIAGVGSEKQPFSVIAKYLSRRQVSLASDPEIPFKLPPTLLLSDAAMEKLFAGS
                     GATYAQTLAKAAGGEMVSRELGREATIHVRVKKEQGTGSNVVGLLEGSDPKLKEEAVV
                     YTAHYDAYGIGADGTIYPGAADNALGASMITAIAEALSKTSPRPRRSVIFLAVTGEEY
                     GLLGANHWVNNPTWPLAKVAANINHDSAGTEVYGPVKRLLGWGQEHSGLGPVFEEAVA
                     ATGNIVTPDPLPEENVFLRSDHYEFVKKGVPALMLAGGPAGDPAVWIA"
     gene            complement(1725..4442)
                     /locus_tag="VF240_16585"
     CDS             complement(1725..4442)
                     /locus_tag="VF240_16585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7176880.1"
                     /translation="MPPAAGRSRRGARIVKFEEQPEGFRRRWTRRLFRPAIVIPLVFL
                     TTIALGLLGYYYYVFSQRIDRLLRGEVFTRSAGIYGAPKEIRVGDNFSAEDLVARLQR
                     VGYVDKAQQADSSRGRYSTNGSVLDVEPSKDATIDGRRQFARLRVQFGKGGRSIAGIS
                     NLDDNGAKAQSAWLEPEQITSVAGTEREKRKVVGYKDLPPHLVKAIVATEDRTFFDHH
                     GLNLRGIVRAFVRRYDSDPNSPIARQGGSSITQQLVKNLFLTPEYSLKRKLAEAYMSV
                     IIETRLSKEEIFALYCNEAYLGQRAGYSIKGFGEAAESYFNKDVTSLTLPESALLAAL
                     IRSPNRYNPYRHPEVATERRNQVLKDMLATGAINEKEAAQSQAEPLKVVRTKGRIDTS
                     EAPYFIDYAQSQLADVISDTSAAERLRIYTTVDLDLQRAAYAAVTKQLAALDKVQSKR
                     FPPGTLQAALVAMRADTGEVVAMVGGRDYEKSQLNRATDAMRQPGSVFKPFVYAAALN
                     TAYDPIPRVITPATVYIDEPKTFSFGTQEYSPGNFGDQYTNGPVTLRDALVKSLNVIT
                     VEVAQEVTVGRVTSIASKAGLPKPKQNYLAHALGTNEATPLQVASAYTAFAQGGRRTA
                     PMAIGRVTTGSGNTVVAPSAQKNEVMTPEVAYVMTSMMKDVVNRGTAAKVRARGFKAN
                     VAGKTGTSRDGWFAGYTPSLVCVVYVGFDDNADLGMTGADSALPIWTDFMQAALAAHP
                     EWGGDWQMPANVQQAEIDPKTGKLAPPESVSKRVELFIANTAPVEESVGSEDDTLGEG
                     EEGEGDGVDEPAPPEAIVVPPLDDAPPPRERRPGRQGEDDSERPRLSGLVTLDIDPTT
                     GFIATEACPVRRSKTFPIGEEPRRYCGPEYHTGRVIIPSDSRPRRVSPQ"
     gene            4720..5796
                     /locus_tag="VF240_16590"
     CDS             4720..5796
                     /locus_tag="VF240_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEX7176881.1"
                     /translation="MSPVKNLLQRSRSRLAYSIEALAASVAIGTAGFCLIEGWSIGDS
                     LYMTLMTVTTVGYGPPQPLSPAGRNFAIFFMVVGVGTAAYAFSSAVQAFVQSEIIAVF
                     GRRRRQREMSKLHDHFIVCGAGRVGARIVAELQRAGLAFVVIERDPQKAGELNLPDTQ
                     LIVRDATLDETLREAGVERARGLVACLPDDADNLYVVLSARTLNRDLHIVARAVEEAA
                     EPKLIKAGASRVVAPTIIGSHRMATALLQPAVADFMDSVAAADLDLAFEQVEVEADSE
                     LAGRKLRFTPISAELNTVVIAIRRRGGEMIFNPSGDTRIETGDTLIPIGRAESLAAVR
                     RMAKGSSNDSSKFKVQSSKDEPPS"
     gene            5827..6669
                     /locus_tag="VF240_16595"
     CDS             5827..6669
                     /locus_tag="VF240_16595"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7176882.1"
                     /translation="MRILVTGGSGYLGTHVRTFFGAADFSRRTGRDVLSESDASMAAD
                     YDAVIHLAAHLSKDPADAELSFRTNADGAANLLRHMRPSAAFIYASTKDVYGSHADRY
                     EEVPETCSTDYCGQSALEWSKLIGEHYANYHARARGVRSCIFRLSATYARPSVGNEPG
                     FVTHYVESVKRRRPIRLPAGGAPVRDLLHVDDFSRACRAFIDSNLTHAMYNLGGGRAN
                     ALSLRRVVETVGRIIDIEPVIDEAAPGPAPVPFNYTSDLARVRAELGWRPEIGVEEGL
                     RSLL"
     gene            6794..>7078
                     /locus_tag="VF240_16600"
     CDS             6794..>7078
                     /locus_tag="VF240_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176883.1"
                     /translation="MRERSIQPERIKRVVVRGANWVGDAVMTAPALRELRRVLPDAHI
                     ALATRPWAEGIFEGADFIDELLPLSGKSKGATALLSQARELRRRRFDLALL"
BASE COUNT         1143 a         2329 c         2355 g         1251 t
ORIGIN      
        1 gcgatccaga cggcggggtc gccggcgggg ccgcccgcca gcatcagcgc cggcacgcct
       61 ttcttgacga actcgtaatg gtcggagcgc aggaagacgt tctcctcggg cagcgggtcg
      121 ggggtgacga tgttgccggt cgccgcgact gcttcctcaa agacggggcc gaggccggag
      181 tgctcctgcc cccacccgag gagacgcttg accggcccgt agacttccgt ccccgcgctg
      241 tcgtggttga tgttggccgc gaccttggcg agcggccacg tcgggttgtt gacccagtga
      301 tttgcgccga gcagcccgta ctcctcgccc gtcacggcca gaaagatgac ggagcggcgg
      361 ggccgcggag aggtcttcga gagcgcctcg gcgatggccg tgatcatcga ggcgccgagc
      421 gcgttgtcgg cggcgccggg gtagatggtt ccgtccgcgc cgatgccgta ggcgtcgtag
      481 tgtgcggtgt agacgacggc ctcctccttg agcttcgggt cggagccttc gagcaggccg
      541 acgacgttgc tgcccgtccc ctgctccttc ttcacgcgga cgtgaatcgt cgcctcgcgg
      601 cccagctcgc gtgaaaccat ttcgccgccc gccgccttcg ccaaagtctg cgcgtaggta
      661 gcgccggagc cggcgaagag tttctccatc gcggcgtcgc tcaagaggag tgtgggcggg
      721 agcttgaagg ggatttcggg atcggaggcg agcgagacct gtcggcgcga gaggtatttg
      781 gcgatgaccg agaagggctg cttctcactc ccgacgcctg cgatgagcag gccgccgatg
      841 ccgcgtccga agaggccgac gaccttcgcc tgcgggctgg aagctttggc ccacgcggcc
      901 tcgtcaacgc cacggggtcg tccgctcagc atgacgacga tcttgccctt gaggtcgagg
      961 ccggcgaggt cgtcgcgctt gagttccgtc gaggtgacgc cgtagccgat gaagacgacg
     1021 ggcgcggtca cgtccgcctg ctcgtatgtg aacggcggcg ggaggataaa gtcggagccg
     1081 tgcttgagcg tcgcgtcgcc cgccttgagg aaggtttcgg actgcacctc ggtggccttg
     1141 aacttgacgg cctgaagata tgtgccggcg tcgccgaggg gcttgagacc gaaggcggcg
     1201 aacttgtcgg ccaggtactt cgcggcgcgc tcgccgccgg cctgcgccgt gccgcgcccc
     1261 tccatctcct tcgaggagag ccgggtgacg acctcgcgga cggtctccag cttgacgcgc
     1321 tcgatggccg cgcgctcggc ggcggtcagc gtcgccgttg cgggtttctg cgtggtcgcg
     1381 gtcgcggcgg ccggctgctg cgcggccggc gcgggggcgg cgacgagttg gagggcgagc
     1441 agcgcggggg cgagtccgcg aaacaggaaa tctttacgag acgatggacg cattaaaaaa
     1501 ttctccaatc ggttcgattg ccaaagtggg agtgcttata cggctcttag ttgttaaagg
     1561 ttgccggaag gcaaagatga aaacagtacc gcgagcggta gcgagcgggt ctggatacgc
     1621 agggcgagac tccggtcgca ggcgcacccg ctcgctaccg ctcgcggtac tgtgcttgag
     1681 tcaatgagcc ggtgagtcaa tcacttcatc cctcatcctt cgcctcactg cggcgagacg
     1741 cggcgcgggc gcgagtcgct ggggatgatc acccggccgg tgtgatactc ggggccgcag
     1801 tagcggcgcg gctcctcgcc tatcgggaag gtcttcgagc ggcggacggg gcacgcctcg
     1861 gtggcgatga agccggtcgt cgggtcgatg tcgagcgtga cgaggccgga cagacgcggg
     1921 cgctccgagt cgtcctcacc ctgccggccg gggcggcgct cgcgcggtgg tggtgcgtcg
     1981 tccagaggcg gcacgacgat ggcctccggc ggcgcaggtt catcgacgcc gtctccctca
     2041 ccctcttcgc cctcgccaag tgtgtcgtct tcgctcccga cactctcttc gacgggcgcc
     2101 gtgttagcga taaatagctc cacgcgtttc gagaccgact cgggcggcgc gagcttgccg
     2161 gtcttcgggt cgatctcggc ctgctggaca ttagcgggca tctgccagtc gccgccccac
     2221 tcggggtgcg cggcgagcgc cgcctgcatg aagtcggtcc agatggggag cgccgagtcc
     2281 gcgcccgtca tgccgaggtc ggcgttgtcg tcgaagccga cgtagacgac gcagacgagg
     2341 ctcggcgtgt agccggcgaa ccacccgtcg cgactcgtcc cggtcttgcc ggcgacgttg
     2401 gccttgaagc cgcgcgcgcg caccttcgcg gccgtgccgc ggttgaccac gtccttcatc
     2461 atcgaggtca tcacgtaggc gacctcgggc gtcatcactt cgttcttctg cgcgctgggc
     2521 gcgaccacgg tgttgccgct gcccgtggtg acgcggccga tggccatcgg cgcggtgcgc
     2581 cgcccgccct gcgcgaaggc cgtgtaggcc gaggccactt gcagcggcgt cgcctcgttc
     2641 gtccccagag cgtgcgcgag atagttctgc ttcggcttcg gcagacccgc cttcgacgcg
     2701 atactcgtca cgcgcccgac cgtcacttcc tgcgccacct cgaccgtaat cacgttgaga
     2761 ctcttaacga gcgcatcgcg caacgtgacg gggccgttcg tgtactggtc gccgaagtta
     2821 ccgggcgaat actcctgcgt gccgaagcta aaggtcttcg gctcgtcgat gtagacggtg
     2881 gccggcgtga tgacgcgcgg aatggggtcg taggcggtgt tgagcgcggc ggcgtagacg
     2941 aagggcttga agacggagcc gggctggcgc atggcgtcgg tggcgcggtt gagctgactc
     3001 ttttcgtagt cgcgcccgcc gaccatcgcc accacctcgc ccgtgtcggc gcgcatggcc
     3061 acgagcgccg cctgaagcgt gcccggcggg aaacgcttcg actgtacttt gtcgagcgcc
     3121 gcgagttgct tggtgacggc ggcgtaggcc gcgcgctgta agtcgagatc gaccgtggtg
     3181 tagatacgca ggcgctcggc cgccgaggtg tcggagatca cgtcggcgag ctgcgactgc
     3241 gcgtagtcga tgaagtaggg cgcttctgac gtgtcgatgc ggcccttcgt gcggacgacc
     3301 ttgagcggct cggcttgcga ctgcgcggcc tccttctcgt tgatggcgcc ggtggcgagc
     3361 atgtccttga gcacctgatt gcggcgctcg gtcgccacct cggggtgtcg gtaggggttg
     3421 tagcgattcg ggctgcggat gagcgcggcc aggagcgccg actcgggcag cgtcaaactc
     3481 gtcacgtcct tgttgaaata ggattcggcc gcttcgccga agcccttgat ggagtagccg
     3541 gcgcgctggc cgaggtatgc ctcgttgcag tagagcgcga aaatctcctc cttcgagagc
     3601 cgcgtctcga tgatgacgga catgtaggct tcggcgagct tgcgctttaa ggagtactcg
     3661 ggcgtgagga agagattttt gacgagctgc tgcgtgatgg aggagccgcc ctgccgcgcg
     3721 atgggcgagt tggggtcgga gtcgtagcgg cggacgaagg cgcggacgat gccgcggagg
     3781 ttgaggccgt gatggtcgaa gaaggttcta tcttcggtgg cgacgatggc cttgacgaga
     3841 tgcggcggga ggtctttgta gccgaccacc tttcgcttct cgcgctccgt gccggccacg
     3901 gaagtaattt gctccggctc aagccacgcg ctctgcgcct tggccccgtt gtcgtcgagg
     3961 ttgctgatcc cggcgatact ccggccgccc ttgccgaact gcacgcggag acgggcgaac
     4021 tgccgccggc cgtcgatggt cgcgtccttg ctcggctcca cgtcaaggac ggagccgttc
     4081 gtcgagtagc gcccgcgcga gctgtcggcc tgctgcgcct tgtccacgta gccgacccgc
     4141 tggagacgcg cgaccaaatc ttcagccgag aagttatcgc cgacgcgaat ctccttcggc
     4201 gcgccgtaga tgccggccga gcgcgtgaag acttcgccgc gcagcaagcg gtcgatgcgc
     4261 tgcgagaaga cgtagtagta atagccgagg aggccgaggg cgatggtcgt gagaaaaacc
     4321 agagggatga cgatggccgg gcggaagaga cggcgcgtcc agcggcgacg gaagccctcg
     4381 ggctgctcct cgaatttgac gatgcgcgcg ccgcgcctgc tcctgcctgc tgcgggcggc
     4441 acgtgccctg tcggtgggtg ccggacggcc atcaagtgaa tagtaagtcg taaatcgtga
     4501 atcgtaaatc gtaaatcgta aatcgttaaa agccgtgaac cgtgaatcgt caacaaccgg
     4561 acgcgagaat tgcccgccct gcctcgataa ttaaccgctc aacgcccgaa aagctcaacc
     4621 gcgattcacg atttacgatt gacgattcac gacattattc gttaccgtgg ccttctcgtt
     4681 gtcggccgtc cgattcgatg cccgcggtgg aagcccacga tgtcccccgt caaaaacctt
     4741 ttgcagcggt cgcgctcgcg cctcgcctac tccatcgagg cgctggccgc ctccgtcgcc
     4801 atcggcaccg cgggcttctg cctcatcgag gggtggtcaa tcggcgacag cctctacatg
     4861 acgctgatga cggtgacgac cgtcggctac gggccgccgc agccgctctc gccggcgggt
     4921 cgcaacttcg ccatcttctt catggtcgtc ggcgtgggca cggccgccta cgccttttcg
     4981 agcgccgtac aggcgttcgt ccaatcagag atcatcgccg tcttcggcag acggcggcgg
     5041 caacgcgaga tgagtaagct ccacgaccac ttcatcgtct gcggggcggg gcgcgtcggc
     5101 gcgcgcatcg tcgccgagct gcaacgggcg ggtctcgcct tcgtcgtcat cgagcgcgac
     5161 ccgcagaagg ccggcgaact gaacctgccc gacacccaac tgatcgtccg cgacgccacg
     5221 ctcgacgaga cgctgcgcga ggccggcgtg gagcgcgccc gcggcctcgt cgcctgtctg
     5281 ccggacgacg ccgacaatct ctacgtcgtg ctctcggcgc gcacgctcaa ccgcgacctc
     5341 cacatcgttg cccgcgccgt cgaggaggcg gccgagccga agctcatcaa ggccggcgcg
     5401 agccgcgtcg tcgccccgac gatcatcggc agtcaccgca tggcgacggc gctcttgcag
     5461 cccgcggtcg ccgacttcat ggactcggtc gccgccgccg acctcgacct cgccttcgag
     5521 caggtggagg tcgaagccga ctccgaactc gcggggcgca agctgcgctt cacgcccatc
     5581 agcgcggaac tcaacacggt cgtcatcgcc atccgccgcc ggggcggcga gatgatcttc
     5641 aacccctcgg gtgacacgcg catcgagacc ggcgacaccc tcatccccat cggccgcgcc
     5701 gagtcgctcg ccgccgtcag acgcatggcg aaagggagtt caaacgacag ttcaaagttc
     5761 aaagttcaaa gttcaaagga cgagccgccg tcttgacttt gaactttgaa ctttgaactt
     5821 tgaactatgc gtattttagt gacaggcggc agcggctacc tcggcacgca cgtccgaacc
     5881 ttcttcggcg cggccgactt ctcacgccgc acgggccgcg acgtgctctc cgagtcggac
     5941 gcttcgatgg cggccgacta cgacgccgtc atccatctcg ccgctcacct cagcaaagac
     6001 cccgccgacg ccgaactctc tttccgcacc aacgccgacg gagccgccaa cctgctgcgc
     6061 cacatgaggc ccagcgccgc cttcatctat gcctcgacga aagacgttta cggctcccac
     6121 gccgaccgct acgaagaggt gcccgaaacc tgctcgaccg actactgcgg ccagtcggcc
     6181 ctcgaatggt cgaagctcat cggcgagcac tacgccaact accacgcccg cgcgcggggc
     6241 gtgcgctcct gcatctttcg cctgtcggcc acatacgccc gcccgtccgt aggcaacgag
     6301 ccgggtttcg tcacgcacta cgtcgagtcg gtcaagcgac gccgccccat ccgtctcccc
     6361 gccggcggcg cgcccgtgcg cgacctgctc cacgtggacg acttttcccg cgcctgccgc
     6421 gccttcatcg actccaacct aacccacgcc atgtataatc tcggcggcgg gcgcgcgaac
     6481 gctctgagcc tgcgccgcgt cgtcgaaacc gtcgggcgca taatcgacat cgagccggtc
     6541 atcgacgaag ccgcccccgg ccccgcgccc gtccccttca actacacgag cgacctcgcg
     6601 cgcgtccgcg ccgaactcgg ctggcggccg gagattggcg tcgaagaagg gcttagaagt
     6661 cttctgtgaa tgaaagaaat ataaacatgg atggatgaga acaagtgata agtgataagt
     6721 gataagacaa aacccgtctt ctctttctca tcactcatca cttatcactc atcactccat
     6781 gtaatgattt gccgtgagag aacgcagcat tcagcccgaa aggatcaagc gcgtcgtcgt
     6841 gcgcggcgcg aactgggtgg gcgacgccgt gatgacggcg ccggcgctca gggagctgcg
     6901 ccgcgtcctg cccgacgcgc acatcgcgct ggcgacgcgc ccgtgggccg aggggatttt
     6961 cgagggcgcc gacttcatcg acgaactcct cccgctcagc ggcaagtcta agggcgccac
     7021 cgccctcctc tcacaagcgc gcgagctgcg ccgccgacgc ttcgacctcg cgctcctc
//
LOCUS       DASVES010000406         8314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_71366, whole genome shotgun sequence.
ACCESSION   DASVES010000406 DASVES010000000
VERSION     DASVES010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71366"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..709
                     /locus_tag="VF240_16605"
     CDS             299..709
                     /locus_tag="VF240_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX7176884.1"
                     /translation="MSKNKRPRTIPMTARVRAELSGVIQGRTAGPVFSSTRTGVNLVE
                     IKKGFKRACELAGIPHGQSTPSGLTFHDLRHTFATRLAERGVGETARMALLGQSNTKM
                     VRRYSHATPEALLEAVQRLEQKAGEVVEFRRKAG"
     gene            complement(797..873)
                     /locus_tag="VF240_16610"
     tRNA            complement(797..873)
                     /locus_tag="VF240_16610"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Met,seq:cat)
     gene            1046..2017
                     /gene="hemB"
                     /locus_tag="VF240_16615"
     CDS             1046..2017
                     /gene="hemB"
                     /locus_tag="VF240_16615"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX7176885.1"
                     /translation="MPELVHRPRRLRRREGLRGLVREARLTRENLVLPLFVCAGTGVR
                     RAVASMPGVFNLSVDEAAREAAAAFDAGVRGVLLFGLPESKDETASGAYARGGVVQEA
                     IRAVRGAAPELVVIADTCLCEYTSHGHCGVVRGGEVLNDESLELLARTAVSQAEAGAD
                     VVAPSAMMDGQVGAIRAALDEAGYEHTAVMSYAVKYASALYGPFRDAADSAPAFGDRR
                     SYQMDPANAREALREAELDYAEGADILMVKPAGAYLDVVRAVRERFDVPLAAYQVSGE
                     YSMIKAAAERGWIDERRVMLETLTGIRRAGADIIITYYAVEAARELR"
     gene            complement(2099..2482)
                     /locus_tag="VF240_16620"
     CDS             complement(2099..2482)
                     /locus_tag="VF240_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176886.1"
                     /translation="MLNASAFALALQAEPQAAEPMRLPLSFMTIVLVLLVGGAVGWLV
                     AAVLGFSRARAFGPSTRWFAFSALCLLLYHIQWFAWVVFGFNQRDRDAMERVIYFGGF
                     FNLFILLGSICAIIGFVQLNKNTDD"
     gene            complement(2700..2918)
                     /locus_tag="VF240_16625"
     CDS             complement(2700..2918)
                     /locus_tag="VF240_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176887.1"
                     /translation="MKSLLDVVLGIVGLAALVFAVYQFYLFAATTDPQGNTPHLWKAI
                     AGAVVLCVCALALFLRHSGSEEEIHITQ"
     gene            complement(3192..5105)
                     /gene="selB"
                     /locus_tag="VF240_16630"
     CDS             complement(3192..5105)
                     /gene="selB"
                     /locus_tag="VF240_16630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX7176888.1"
                     /translation="MLSIVVGTAGHIDHGKTSLVRALTGVDADRLPEEKRRGITIDLG
                     FAELDLGDVRVGFVDVPGHERFVKNMLAGAHGLDAVALVIAADEGVMPQTREHFDICR
                     LLGVGAGLVVLTKTDAADAELLELVRTEAEELVKGSFLEGAAVVAVSSRTGAGVEELK
                     TRLREVALAAPARSAGWVARLPVDRAFTVRGFGAVVTGTLVAGEIAEGEELELLPARA
                     RVRVRGLQVHGASVGAARAGQRTAVNLGGVEAASVERGMVLAPPGRLRPTQMFDAEVE
                     VLAAASRPLRTRQRVRVHAGAAEVLGRVAVVEESGEVAPGGRGFAQFRLESPIVVVPG
                     ERFIVRAYSPQRTIAGGLVIDAHAAKRRGRERREARARLDALAAGDDAARLALYVEAA
                     GALGLSHADLAARTGWTDEALAAAAGEAQGRGAVVETEGVFVGRDVLGQLAAGAVAEV
                     EAFHAREPLARGLARETLRERVFAHAAPEVFRAALRRAETEGALASERDVVRSGRHSR
                     ALTPADEALRDRLEKIYVETALEPPALDDAFERAGAGARRDHARKILQLLIDDRALVR
                     VRDDLFFHRASIDGLVAALRAYAARHEPERLIDVAAFKELSGVSRKYAIPLLEHFDRE
                     RVTRRAGDRRIIL"
     gene            5507..6733
                     /locus_tag="VF240_16635"
     CDS             5507..6733
                     /locus_tag="VF240_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176889.1"
                     /translation="MPDSVRRGLAEGYGADALSSEDAESGESERQQQQQTRLRLLQQR
                     AVDDDGDGARCGLPTLATAGDVREVVQYLKKKPEGVSVLEAVEDVRRRIFEPRKVAAY
                     EMWGLVERRNARLLLTPLGREFARRLEPESEAYRALLRRTEAYHAALLWMRRQHADII
                     THTEVCAFWQQLFGAEAHAQTNEGHVVCFFHLCQAAQLGAVTVGKRGQPARLRIDREE
                     LADYLETEGERASAEAPAAAAESDARPARPHTFGDDEETRPAAAGGALRIFVSAGRAS
                     ALAAQVETTLAIAGDVCEVFTRGDADSLLPPEQQARAMREADAAVFVVSSEDYRPDES
                     GEHVIRQALLIQIAVAVILYDGRVALLCETGCCVPPSLSGLRRFAFADDVLTWEVGIG
                     LLKAAKEFQTGTSRAA"
     gene            6773..8191
                     /locus_tag="VF240_16640"
     CDS             6773..8191
                     /locus_tag="VF240_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176890.1"
                     /translation="MKTLFVIVVFFCCLLPAAAAQSPSSAHAEELPRGQIIERLAARD
                     DPSQSYALYLPSHYSTNRAWPILYCFDPLGRGSVPVERFREAAEKYGWIVVGSNNSRN
                     GPLAPSVAAARAVWADTRARLSIDDRRLYAAGFSGGARHAVRVNQLCQNCLAGVIACG
                     AGFPPDIDPSASSRFALFGMAGADDFNFPELKLLDERLARLGFPHRLEVFDGGHSWAP
                     AHVCTKAVEWMELQAMRAGRRTRDEKLIGELWDKMLAKAVDDEAAGRVYDAWVAYRSL
                     AEDFEGLRDVAGEAGRAARLGESKEVRRALDEEGAQIRRQQNLFDRIRALHESRSGAE
                     EPAVVLMEFRRAVAALGKAAGAGGDTGERRVARRTLRQLFAHYYESAANLRDRGVESG
                     RVVSHLEVAAEIIPDNPQVLFELASAYAVNREKKKALTTLRKSVESGFTDADAVAGNE
                     SLAPLRDEAAFKEIVERLREKP"
BASE COUNT         1431 a         2642 c         2906 g         1335 t
ORIGIN      
        1 aggaagtcgc cggaccgtac ggaaagccgg cccgtgtcgc acatgagggc ggcctgttct
       61 accgccctcg tctcagcagg cacttgggtc ggacgaactt cgtctccaca cagggtacta
      121 tacaaactat gctccggtta atcttgggct aaagaatgga acatcaacag tgactggtgt
      181 ctggattttg ccgttggggc tatgcggtta tggctgttcg atagaggcgc acgagaacac
      241 aaactttggg aatctgcgct gaaaagcaag tcggagggtt ttatctggga ggcagtagtt
      301 gagcaagaat aagagacccc gcaccatccc gatgactgcg cgggtgaggg ctgaactctc
      361 gggtgtgatt cagggtagga cggcggggcc ggtgttttct agcacccgga ctggagttaa
      421 ccttgtcgag atcaagaaag gcttcaagag ggcgtgcgag ttggcaggca ttccgcacgg
      481 gcaaagtacg ccgagcgggc tgacatttca cgacctgaga cacaccttcg ccacccggtt
      541 agccgagagg ggcgtggggg agacggcaag gatggctctg ctcggacagt cgaatacgaa
      601 gatggttcgc cgttacagtc acgccacgcc tgaggcattg ctggaggccg ttcaaaggct
      661 tgaacagaag gcgggcgagg tggtggaatt tcggcgcaag gcgggatgag ggtcaccatt
      721 cggtcgccat ccggccgagt gagaagggcc ggtcgcgccc cggccctccg taaacgattg
      781 acagtgtgaa ggtaattggt agcggggggg agacttgaac tcccgacctc ggggttatga
      841 atcccgcgct ctaaccggct gagctacccc gccatgaaga tgggggttaa ttatagggac
      901 gtgatattaa aggtcaagcg ggttgtggga agcggcgtgg gtattggcgc gcgaggaagc
      961 gggtaagtgt cgggtcgttt gcggcggtgt gtgggggcgc gtatcatcgc ggcgtaaact
     1021 catcaactca tacggggatt gtttgatgcc tgagctggtt cacaggccgc gacggctgcg
     1081 gcgacgggag gggttgcggg ggctggtgcg tgaggcgcgg ctcacgcgcg agaatctggt
     1141 gctgccgctg tttgtctgcg cgggcacggg cgtgcgccgc gcggtggcct cgatgccggg
     1201 cgtcttcaat ttgtcggtgg acgaggcggc gcgcgaggcg gccgcggcct tcgacgccgg
     1261 cgttcgtggc gtgctcctct tcgggctgcc cgagtcgaag gatgagacgg cctcgggcgc
     1321 ctacgcgcgg ggcggcgtgg tgcaggaggc gattcgcgcg gtgcgaggcg cggcccccga
     1381 gttggtcgtc atcgccgaca cgtgcctgtg tgagtacacg tcgcacggcc actgcggggt
     1441 ggtgcgcggg ggcgaggtct tgaacgacga gtctttggag ttgctggcgc gcacggccgt
     1501 cagtcaggcg gaggcggggg ccgacgtggt cgcgccctcg gcgatgatgg acggtcaggt
     1561 aggagccatc cgcgccgcgc tggacgaagc aggatacgag cacaccgccg tcatgtccta
     1621 cgccgtgaag tacgcctcgg ccctctacgg gccgttccgc gacgcggccg acagcgctcc
     1681 ggccttcggc gaccgacgct cctaccagat ggatcccgcg aatgcgcgcg aggcgttgcg
     1741 cgaggccgaa ctcgactacg cggagggcgc cgacatcctg atggtcaagc cggcgggcgc
     1801 ttacctcgac gtggtgcgcg ccgtgcgcga gcggttcgat gtgccgctgg cggcctatca
     1861 ggtgtcgggc gagtattcga tgatcaaggc ggcggccgag cgcgggtgga tcgacgagcg
     1921 gcgcgtgatg ctggagacgc tgacgggcat ccgccgcgcc ggcgccgaca tcatcatcac
     1981 gtactacgcc gtcgaggcgg cgcgagagct aaggtaaaag taaaaaggca aaaggcaaaa
     2041 gtgtggggca cacatgcttc gtcacttttg ccttttgcct ttttactttt accttgtctc
     2101 agtcgtctgt atttttgttg agttggacga agccgatgat ggcgcagatg ctcccgagca
     2161 ggatgaagag gttgaagaag cctccgaagt agatgacgcg ctccatcgcg tcccggtcgc
     2221 gctggttgaa gccgaagacg acccacgcga accactggat gtggtagagc agcaggcaga
     2281 gcgccgagaa ggcgaaccag cgcgtcgagg ggccgaaggc gcgcgcgcgc gagaagccga
     2341 gcaccgcggc gacgagccag cccaccgccc cgcccaccag cagcaccagc acaatcgtca
     2401 tgaaggagag cggcaggcgc attggctctg ctgcctgagg ctctgcctga agggcgaggg
     2461 cgaaggcgga agcgttgagc atcgaaggat tcatctcctg tcggaatgtg tgtaaaggta
     2521 aacgtaaagc cgcccgcatc cgctcggggc acgtcggccc cgggcggggt tgcaagcggc
     2581 gtccgtggtc taaaatcgat gtcggccgaa ggagccgagg cggcatgctc cgcgcgcggc
     2641 cgcgggcgtc gccgccaagc ggccgcgccg gtcttatgcg tcggcgaccg tcggcgcact
     2701 cactgcgtga tgtgtatctc ctcctcgctg cccgagtggc ggaggaagag cgcgagcgcg
     2761 cacacgcaca ggacgaccgc gccggcaatc gccttccaga gatgcggcgt gttcccctgc
     2821 gggtcggtag tcgccgcaaa caggtagaat tgatagaccg cgaacacgag cgcggccaaa
     2881 cccacgatcc caagcacaac gtccaacagg cttttcatgt tacctccagc gtccgctacg
     2941 cccagatagt caacggcttg aaagataaag ccttgtgagt ccgatgaatg tgtcgggttc
     3001 tcgcttgaag aaactgccag aattcgccgc ctatgttagc gccctcgcct gtcagaagca
     3061 agcccttcgt aaatcgtcaa tcgtgaatcg tacatcgaaa aggctggggc agccctccgc
     3121 cttgcgctgt cctgctcagg ccgaatgtcg ttcaaacacg attcacgatt gacgattgac
     3181 gatttacggc cttaaagaat tattcgccgg tcgccggcgc ggcgcgtgac gcgctcgcgg
     3241 tcgaagtgtt cgaggagcgg gatggcatac ttgcgcgaca caccggagag ttccttgaag
     3301 gcggcgacat cgatgagacg ctcaggctcg tggcgcgcgg cgtatgcgcg cagggcggcg
     3361 acgagaccgt cgattgacgc gcggtgaaaa aagaggtcgt cgcggacgcg cacgagcgcg
     3421 cggtcgtcga tgagaagctg gaggattttg cgtgcgtggt cgcgacgtgc gccggcgccg
     3481 gcgcgctcga aggcgtcgtc gagcgcgggc ggctccagcg ccgtctcgac gtagattttt
     3541 tccagacggt cgcgcagggc ctcgtcggcc ggcgtgagcg cgcggctgtg gcggccggag
     3601 cgcaccacgt cgcgctcgga ggcgagcgcg ccctccgttt cggcccgccg gagcgcggcg
     3661 cggaaaactt cgggggcggc gtgggcgaag acgcgctcgc gcagcgtctc gcgcgcaagg
     3721 ccccgcgcca aaggctcgcg cgcgtgaaac gcttcgacct ccgcgaccgc tccggccgcc
     3781 agttgcccga gaacatcgcg cccgacgaag acgccctcag tctccacgac ggcgccgcgc
     3841 ccctgcgcct caccggcggc ggcggcgagc gcctcatccg tccagcccgt gcgcgccgcg
     3901 aggtcggcgt gcgacaggcc gagcgcgccg gccgcttcga cgtagagtgc gagtcgcgcc
     3961 gcgtcgtcgc ccgcggcgag tgcgtcgagg cgcgcgcgcg cctcgcgccg ctcgcgtccg
     4021 cgtcgcttcg cggcatgcgc gtcgatgacg aggccgccgg cgattgtccg ctgcggcgag
     4081 taggcgcgga caatgaaccg ctcgccgggg acgacgacga tgggcgactc cagacggaac
     4141 tgcgcgaagc cgcgcccgcc cggcgcgacc tcgcccgact cttcgacgac ggcgacgcgg
     4201 cccagcactt cggccgcgcc cgcgtggacg cgcacgcgct ggcgcgtccg cagcgggcgc
     4261 gaagccgccg cgaggacttc cacttcggcg tcgaacatct gcgtcgggcg cagacgcccc
     4321 ggaggcgcga gcaccatccc gcgctccacc gaggccgctt cgacgccgcc gaggttcacg
     4381 gccgtgcgct gccccgcgcg ggccgcgccg accgacgcgc cgtgtacctg tagcccacgc
     4441 accctcacgc gcgcgcgcgc cggcagaagc tccagctcct cgccctcggc gatctcgccc
     4501 gcgacgagcg tgcccgtcac caccgcgccg aagccgcgca ccgtgaaggc gcgatccacc
     4561 ggcagacgcg cgacccaccc ggccgagcgc gctggcgccg cgagcgctac ctcgcgaagt
     4621 ctcgtcttca actcttcgac gcccgcgccc gtccgcgagc tgacggcgac cacagccgcg
     4681 ccttcgagga acgacccctt taccagctcc tcggcctccg tgcggacaag ctctaagagt
     4741 tccgcgtcgg ccgcgtcggt cttggtgagc acgacgagtc cggcgccgac gccgagcaag
     4801 cggcagatgt cgaagtgctc gcgcgtctgc ggcatgacgc cctcgtcggc ggcgatgacg
     4861 agggcgacgg cgtcgaggcc gtgcgcgccg gcgagcatgt tcttgacgaa gcgctcgtgg
     4921 ccgggcacgt ccacgaaccc gacgcgcacg tcgcccaagt cgagttcggc gaagccgagg
     4981 tcgatggtga tgccgcgccg cttctcttcg ggcaggcggt cggcgtccac gcccgtcagg
     5041 gcgcgcacga gagaggtctt gccgtggtcg atgtgtccgg cggtgccgac gacgatggag
     5101 agcatgggtg ggtgaatcgt aaatcgtgaa tcgtaaatcg tcaaaagccg tcaatcgtga
     5161 atcgtatatc gtaaagccgc gcccggcgtc ggccgacgtg gctactcaag actcgcgcgt
     5221 caaccacgat tgacgattga cgattcacga ttgacggctt tttggcgttt gcaagggctt
     5281 tgcaagcggg ggcggcgctt cactcccgtc caatgttaat ccgcgtcgtc atcaaaagca
     5341 gcctttccat cacaaaatca gttcgggccg tagccgctca ggcatctttg cccgtgcgac
     5401 gggcagtcaa aggaagcgtc catccgttca gggaacgagc acgggtgaaa atcgagcagc
     5461 gaagggaagg aagcactccc ccatgtcaag gttgttgagt tcgacgttgc cggacagtgt
     5521 gagacgcggc ctcgcggaag gctacggcgc ggacgcctta agctcggagg atgcggagag
     5581 cggcgagagc gagcgccagc agcagcagca gacccgcctg cggctcctgc aacaacgggc
     5641 cgttgacgac gacggcgacg gcgcccgctg cggcctcccg acgctggcga cggccggcga
     5701 cgtgcgcgag gtcgtgcagt atctgaagaa gaagcccgaa ggggtctccg tcctggaggc
     5761 ggtggaggac gtgaggcgtc gcatcttcga gccgcgcaag gtcgccgcgt atgagatgtg
     5821 gggtctggtc gagcgacgga acgcgaggct gttgctgacg ccgctgggcc gggagttcgc
     5881 gcgccggctc gaacccgagt ccgaagcgta ccgcgcgctg ctgcggcgga cggaagccta
     5941 ccacgcagcc ctgctctgga tgcgccggca acacgccgac atcatcacgc acacggaagt
     6001 ctgcgccttc tggcagcaac tcttcggcgc cgaagcgcac gcacagacga acgagggcca
     6061 cgtcgtctgc ttcttccacc tgtgtcaggc cgcgcagctc ggcgctgtga cggtcggcaa
     6121 gcgcgggcag ccggcgcgcc tgcgtatcga ccgcgaagag ctggccgact atcttgagac
     6181 ggaaggagag cgcgcgtcgg cggaagcgcc ggccgccgcg gccgagtcgg atgcacgacc
     6241 cgcgcggccg cacaccttcg gcgacgacga ggagacgcgt ccggcggccg cgggcggggc
     6301 actgcgtatc ttcgtctccg cgggccgcgc ttccgcactc gccgctcagg tcgagaccac
     6361 tctggccatc gccggcgacg tgtgtgaggt cttcacgcgt ggcgacgccg actcgctcct
     6421 cccccccgaa cagcaggcgc gggcgatgcg cgaggcggac gccgccgtct tcgtcgtctc
     6481 gtccgaagac tatcgcccgg atgaatccgg cgagcacgtc atcaggcaag cgttgctcat
     6541 ccagatcgcc gtggccgtca tcctctacga cggccgtgtc gcgctgctgt gtgagacggg
     6601 gtgctgcgtg ccgccgtccc tgtcagggct gcgacgcttt gcgttcgccg acgacgtgct
     6661 gacgtgggag gtcggcatcg ggctgctgaa ggccgcgaaa gagtttcaga caggcacatc
     6721 ccgcgcggcc tgacctgact tctaatggcg accctcattc gcccgccgcc cgatgaaaac
     6781 tctgttcgtc atcgtcgtct tcttctgctg cctcctgccc gccgctgccg cgcagtcgcc
     6841 gtcgagcgct cacgcggagg aactgccgcg cggacagatt atcgaacgtc tcgccgcgcg
     6901 cgacgatccg tcgcaaagct acgcgctcta cctcccgtcc cattattcga caaatcgcgc
     6961 gtggccgatt ctctactgct tcgaccctct cgggcgcggc agtgtccccg tcgaaaggtt
     7021 ccgcgaggcg gccgagaagt acggctggat cgtcgtcggc tccaacaact cgcgcaacgg
     7081 gccgctcgcg ccgtcggtcg cggcggcgcg agccgtgtgg gccgacacgc gcgcgcgtct
     7141 ctccatcgac gaccgacggc tctacgcggc cggcttctcc ggcggcgcac gacacgccgt
     7201 ccgcgtcaac cagctctgcc agaactgtct cgccggggtc atcgcctgcg gcgccggctt
     7261 cccgcccgac atcgacccct cggcctcctc ccgcttcgcc ctcttcggca tggccggcgc
     7321 cgacgacttc aacttccccg agttgaaact cctcgacgag cgtctcgccc ggctcggctt
     7381 cccacaccgc ctcgaagtct tcgacggcgg ccactcgtgg gcgcccgcgc acgtctgcac
     7441 gaaggccgtt gagtggatgg agcttcaggc gatgcgcgcg gggcggcgca cgcgtgacga
     7501 aaaactcatc ggcgaactct gggacaagat gcttgcgaag gccgtggacg atgaggcggc
     7561 cgggagagtc tacgacgctt gggtcgccta ccgttcgctc gcggaagact tcgagggctt
     7621 gcgcgacgtg gcgggcgagg cgggcagggc tgctcgcctc ggcgagtcga aagaggtgag
     7681 gcgggcgctc gacgaagagg gcgcgcagat tcgccggcag cagaatttgt tcgaccggat
     7741 tcgggcgctc cacgagtcgc gctcgggcgc ggaggagccg gcggtcgtgt tgatggaatt
     7801 ccgcagggcc gtcgctgcac tggggaaggc ggccggcgcg ggcggcgaca cgggcgagcg
     7861 ccgcgtggcg cgacgcacgc tgcgccagtt gttcgcgcac tactacgagt cggccgcgaa
     7921 ccttcgggat cgcggcgtgg agagcgggcg cgtcgtctcg cacctcgaag tggcggcgga
     7981 gattatccct gacaacccgc aggttttgtt tgaactcgcc agcgcctacg cggtcaacag
     8041 ggaaaagaag aaggcgctca cgacgctcag gaaatccgtg gagagcgggt tcaccgatgc
     8101 ggacgccgtc gccggcaatg agtcgcttgc gccgctacgc gatgaagcgg ctttcaaaga
     8161 gatcgtcgaa cgcctgcgag agaagccgtg acacctgtcg ggtggaagag ctttcctgcc
     8221 ttaagagaat caaacttgaa tctaaggtaa gtcggactca ccgcagaggc gcagaggacg
     8281 cagaggaggc gcagagggtc gagcagttca ctcg
//
LOCUS       DASVES010000407        19429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1219513, whole genome shotgun sequence.
ACCESSION   DASVES010000407 DASVES010000000
VERSION     DASVES010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19429
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219513"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..679
                     /locus_tag="VF240_16645"
     CDS             491..679
                     /locus_tag="VF240_16645"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HEX7176891.1"
                     /translation="MPASEKQTENKRPDGKFAEGNKLGRRFRPGQSGNPKGRPKSITL
                     SEAYRKLLAQVDERPRRE"
     gene            1383..4340
                     /locus_tag="VF240_16650"
     CDS             1383..4340
                     /locus_tag="VF240_16650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7176892.1"
                     /translation="MKVFGGYEIEGELGRGGMGVVYLARQIELNRRVALKMLTGHHGP
                     DELQRFLEEAETAAGLNHTNIAHIYEIGEHDGAPFFSMEFVEAGSLADRLRKELPSPE
                     DAAQLLVNVARALHFAHQNGVVHRDMKPANVLLDPDGVPKVTDFGIAKRLKADANLTR
                     SGVVIGTPTYMAPEQAKGNSRHVGPAADVYSLGAILYEMLAGRPPFLPEDSETAITVR
                     VLTEDPVSPAYHRPEIPRDLETICMKCLGKEPRDRYASAAALAEDLRRYLDDEPILAK
                     PPNTVSSSIKWVRRNPWKFVATATTLLLAVAGLALLARWELYQRPRLEYAAQVAWVNG
                     CLEPLEKIGEENASQRAAYLRLTRRGRFGPIVKAEVLNARGHPAVLRRILNPEMIPIY
                     MEGLAGAQLYAEARQETTKVEFWFDERNQAREATGRDRNGQVNWRMLYDHQDASGSFG
                     HTARARFVNMRGFDATSRDGASHMEFERDAKGRDMKISFFNAAGKPAANGEGVYGYTL
                     ERDDAGRIVRLVNLGLDGQPAANRVGMIAFNMSWGREDRLEVRDAKGQPSVWNGVAAV
                     VRENDGSGNPVRVTNLDPEGKPVSAAETEWSVQEMKRNERGELTQRTYFKADTDGALK
                     QISQVNVTYDDFGHPADVQFAGATSWRSALRHDANGNVIEEKLLDAEGKPRAGDKGYA
                     IKRFTYTSGSSGVRIEQTYFDAAGEKTYSTGAGGYHRLIDEFDATGALRRQTMDEHDP
                     ARFKYYRYVSEPEYDPQGRLRHNVSRFEDAQGNLATDADLLFTATEDFYDENGRLTTE
                     WKIGCALEDFGGPVLRLDTEWHSNGKMKRWVKQICDANRQPLSFLSNGNGARHEEEYD
                     AKEQRERIYDTGFDEKLVGFSTREAKFSGGNLQSVTHTRSDGTVLDSVRVIITAVAPP
                     EGQPKSAELRAGDQLIASNGKSVTSAYAWVTGGFAGGWIEVLREGRRIRIDGFGPGVL
                     GVDLQDRAPAGKQ"
     gene            4432..5067
                     /locus_tag="VF240_16655"
     CDS             4432..5067
                     /locus_tag="VF240_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176893.1"
                     /translation="MADSPTTPPGRKAPVKDELNLLEVLRLLIETEKATTTSDLLKYA
                     SQYGAPEAEERLRVLEAENIPLDLAFESISVHIRLLAYKRNSELLAGCRGQKVGLILP
                     LPPHLPNLFLPVADVTFLEPSERTVHGSLHDFMERAVKGARACRAKAEEMQALVFEAF
                     RDDDNLFVEVAVAEVLEPKLLPAGIRLIAHLRPHRNPSDVQIQPAVEVSFI"
     gene            5064..6518
                     /locus_tag="VF240_16660"
     CDS             5064..6518
                     /locus_tag="VF240_16660"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX7176894.1"
                     /translation="MKMPLRFLPLFGVMAVFFAYCAPVWAAGGAPAAGTAPALIDSWE
                     MTWPAGVLMLVLLFGAFFAMTRYEVPLPLAMTLVSLAFLVIQWESAPVILREGFAHYA
                     DITVLFTAVAIPAHMIERSQGFKWVAAWLGHQLGKVRLRHPRLTTPLLVGILLFATYV
                     TAGLMHNVTSILIMTPVIIRLCASYHVPSRWILSAALIASNLGGFSTRWGDTPNIIES
                     ATWGLTAGDFMREVLPANLIVMAALIIVATILTQRGISQRSRQAGVTQVPFYTLQVAQ
                     TAADWKKAKADLAIDRRLLTIGLVSLVFFIVLHVIFTKLAIAIGAITIVTAVLLERPK
                     ERYHTLTSLGFDVYLAFAAIFVLAGCVSHSWIGSTLYNQLIAANAAPWVIAVTGYFGT
                     TFTEAASWATAVSVPIQQLDPSHAAAWALGGGICAGSSSLVTAASAGIILAEESQRFK
                     DPEHAITFRRYLAFGLPFSLFMLVFYSLYFTFVR"
     gene            6529..8349
                     /locus_tag="VF240_16665"
     CDS             6529..8349
                     /locus_tag="VF240_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176895.1"
                     /translation="MAEQRPTAEPSPFLGDLAVVWKRRKQIWRLLPRADKLGFGSGLL
                     IMALVAFLENQIPLLLGKFFDGVTNVAAQPKELMPFVIRALAILTALYIGKESLQLVR
                     RWLVSRTATRIEANMMVRLVGHLLKIDLGALARERVGSLHGRISRSIEGFVKFLRVSF
                     TDFLPAVLTAGVALYYGLATNWKIGLIMVCVVPLSLLITIWQVSSQKGIRESLLRAKE
                     GLDGTVVEQLGGLEYIRAANTYPTEVARIDTAAEARRAREMKHGLAMARFDWAKAINE
                     GLFHVAIIGYAIVLASRGEIEYGTVITFSLLYLNIARPLKEVHRILDETYSSSQQVSV
                     LLEMLNQPQDQSFEVVTVRAPRLDGSIPLLECQDLVVDYTTPEGAPRRILNGLTLAIR
                     QGQTIGIAGRSGSGKSTWLRCMLRLIHPASGEVLVGGVPIGVLSREDIGKSIGYVSQV
                     PFVFSGTVSENIAYGCGATTSDKIQDAARQAHIHEEILQMSQGYDTMLTERGGNLSGG
                     QRQRIALARMFLKNPPVLILDEGTSALDNISERRVRAAIDHARQSHTVIMVAHRLTTL
                     NQTDCIFVFDEGRVVEQGAFDDLVAKNGVFAELVRSAEAT"
     gene            8370..9425
                     /locus_tag="VF240_16670"
     CDS             8370..9425
                     /locus_tag="VF240_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7176896.1"
                     /translation="MSTKSVTFVWPGDLHLETDDRPNYQTAHWMADEVNELIRPDFVQ
                     FAGDNVQHARDKEWELFKNVTSKLKMPFHALVGDHDAHHDPGCHSYQAQLGATYDAFT
                     VGGYRFIRLNTNQYKPVGLSDEQVIWFRYEVDAALARGERVIVFQHNYPFQVWENYAG
                     PGIEEWREIMQTRPVTALFSGHTHYGQMANDGRNVYFAMRSIGDPEGGAAGYAIVHLD
                     GEDLALTYRTREERGPIALITHPRRVILATKPAHIVTGPTECHARGWSATPITSAQAR
                     TDEGDWSEMRQTGEMTWSFPIPGDTLAKGEHTLEVRLIDENKDEGTDRITFACDLSGR
                     YNPYPMVEPVVKGTKFC"
     gene            9703..10479
                     /locus_tag="VF240_16675"
     CDS             9703..10479
                     /locus_tag="VF240_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176897.1"
                     /translation="MSLFGTRHLLVAVSTFIIGIGTFAAIWGMPWVLTRSVTIKSQVA
                     AHLLDDRGAVNGLLLASGDQLRFSPQTGEAVAAQINSGDEVTATGRAGSKSDYGREVR
                     VEQISANGRTIIEAGSGPKHPRKSRDGSMPQGPGGRPAPPAAAAQGGAKTEPNVAPSG
                     DADANQTENGQPAPPETFKATGAIKDHLVNRHGDVDGLILSGGVQVRFSPKVGELVVA
                     AEQGADTQVSIEGTGVRNERGTVMRPALITVGNQTIALGR"
     gene            10608..12311
                     /locus_tag="VF240_16680"
     CDS             10608..12311
                     /locus_tag="VF240_16680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176898.1"
                     /translation="MTTLDAIRKMSLRARLAMLAAFALVALLVALFVAWRLARATEVF
                     ALRQADSSLHAAARDLARAFHAHPSGYQTIEEAAARPDRPAAPRDKGPRERPLPPHVQ
                     TLFAAYRDPLSRLTAATLHRFPETAGGFYRATDGALVGQAFPANAGTGGQMVLASDRT
                     DMIRALAKQVNEAGAPATRAMQTGSDRIIVVAYPVQADSSEVEDISSRADGDSSSPIT
                     AAWTMQRLSNLTGVSDKANLAALAVLILSIIVVSGLALTTVRDLRSGVSGIEQGLAGL
                     TNDLSQQIAPPLTPELARITGAINQLADTLRVNLARQSELEHDLRRSERLSALGRLIA
                     GVAHEVRNPLAAIKLKVQMARRAKSAPDKLDDTFRVITEEIDRLDALVRRLLEFSRTR
                     PPEQSKLDLCELVRHRAALFNDVAERAGVKIETRVSPDGVMIEGNTERLAQVLDNLIQ
                     NALEAMPDGGQLAISCQVVTTEDGLAVARLGVEDTGRGIPPEGHERIFEPFFTARETG
                     TGLGLAIAREIVEAHGGRINFVSRAGSGAFFLIELPQRVQAEDSKRFDQRPGFNSGGA
                     E"
     gene            12308..12634
                     /locus_tag="VF240_16685"
     CDS             12308..12634
                     /locus_tag="VF240_16685"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX7176899.1"
                     /translation="MKTVILLIISNIFMTIAWYGHLKHKERPIVWLILISWSVALFEY
                     IFQVPANRIGSQTYSVTQLKIMQECITLVVFTVIAYFFFDQTLKWNNFVSYALIVGAV
                     YFSFAH"
     gene            12639..14051
                     /locus_tag="VF240_16690"
     CDS             12639..14051
                     /locus_tag="VF240_16690"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7176900.1"
                     /translation="MKEDAAQVLIIDDETNLRESLAELVESEGFRAAQAANGEEAVRV
                     LRAGATVADVVFLDMRMPKLDGLSVLRLIQEEKLTDAPVIVISAFGDSSKMIEAMRLG
                     AYDYITKPLDLDETVAILRRAAEQRRLNREAEALRRDGSTNESAKEAASSDRQFEMLG
                     VSRAMRDIFKQIGRIAATDATLLITGESGTGKELVARAVHRHSARSRGPFVAVNCGAL
                     PENLIETELFGHERGAFTGAERQKKGRFELSHTGTLFLDEVGELSPAAQVKLLRAVEA
                     RRFERVGGTESVGVDVRVIAATNRDLKHEVEEKRFREDLFYRLNVIEVRLPPLRERLA
                     DVPQLAESFLERAVSRHALKTKILSPAALRELLAYDFPGNVRELENMVERAAVTSSGE
                     VILPEHLFGGDHATATIAPQNAESKLFELPFHRAVAALERELIRRALEAAGGNRAGAA
                     RMLGINRRLLYSKMDEHRIT"
     gene            14490..15140
                     /locus_tag="VF240_16695"
     CDS             14490..15140
                     /locus_tag="VF240_16695"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7176901.1"
                     /translation="MTDQLLGLLSTYGLPALFVILAVSSAGVPFPITLLLIASGSFVA
                     QGEMGLWQVLMICSAGAIIGDQIGYWLGRSGGRKFVRRITERFGGAEMIKRAEAFTKR
                     WGGVGIFLSRWLVTPLGPWLNLTSGITGYSWPRFLFWDVLGEVLWVVIYVSLGNIFSD
                     RVGALAELLGNLTWVIVGVIVAVILGWKLLQYFRSGDESSEETKATAVAYKTVVEG"
     gene            16232..16684
                     /locus_tag="VF240_16700"
     CDS             16232..16684
                     /locus_tag="VF240_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176902.1"
                     /translation="MAEAEQHSEQQAVYAQQGDYEAAREAADNAAYATYEADSGAGGD
                     DHMGQADAEKYNMDWAVHEEKQADYYADNAAAYAAEGDFENAERSASSAAAHQESADH
                     FGDLGEHGGDLAVYDPSSAVATGGLTNRLSIRPPPKWIRGSIRGWIRV"
     gene            complement(16883..17635)
                     /locus_tag="VF240_16705"
     CDS             complement(16883..17635)
                     /locus_tag="VF240_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176903.1"
                     /translation="MKITALLACTILLAAPSLAQRKSHDYKLPTGALIIETQPVKTNR
                     ALILWVLNPRRIPRDLPDEPYTCPEETRGSYYSGATRVSLVDTRTRRIINTLAVRQEY
                     NEGVDEFDTPYQIHSGSHYRVGDVPAGREGKPTIMWLRDYNGDGRALEFALFDALACM
                     GLQTTLIGYSERQDKVIQYPVRLVVEDKGKRSVEIRRWVDYLFSGKPKGAGYWRYEID
                     YRGRGGPLAKYEIRYNRRAERFEGKVVYTEGE"
     gene            complement(17795..18391)
                     /locus_tag="VF240_16710"
     CDS             complement(17795..18391)
                     /locus_tag="VF240_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176904.1"
                     /translation="MISGTGTQVFIPKKSPAGGILLWAVLHLILGFVAWFVLTTILMS
                     IVERVVGPVGEVGLADVIVVLFLLSGTIVVPLCVFLRWYVATTLAIALDAETGITLTN
                     KSMRRGEQIKHYHWQDVTGTQMIGTTSGEGIHTEYTFYVYTADGGEIKIPRSMGSFDY
                     FLDIVNRATTHLTYTWMYVDYTIAGRDQGWHQVTRAQG"
     gene            complement(18985..19260)
                     /locus_tag="VF240_16715"
     CDS             complement(18985..19260)
                     /locus_tag="VF240_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176905.1"
                     /translation="MRVPVEQVPWTLRDVEHIPPEEIQAAMLLIVGHAVGIGVDSLLS
                     ETARLFGFSRVGDRIRERLLGQLKSLTRARKLALSGDSVTLRGTAAR"
BASE COUNT         4025 a         5854 c         5748 g         3802 t
ORIGIN      
        1 agaagcaaga gcaaatttca aacagggttg agcgtcgggc gagttcattt caacacagag
       61 ggcgcaaagg gcacagagcg cacagagaaa gacaaattgc ttcctctgtg cgctctgtgc
      121 cctttcccca cacaagctga acggcggccc ccgagggccg ccgccggctg acgtaacctg
      181 ttgaatttgt tggtagcggg ggcgtgactc gaacacgcga ccttggggtt atgaatccca
      241 gaagaggcac accacttacc ctcacgtaaa cgaatacaac cgaatgaaac ggacttcgta
      301 gccgccagag tgggttaggt tacatgaggt taagtttcat aaggtcacca tcctgacgcc
      361 atcggtatga agacgttggt ggcgacgaag agctgcgccg cctgcgtacg atgacagatg
      421 gttcgcacaa gtgggaagag tcagggttgt gactcggctt tttccataat ctcgccttgg
      481 aggtgtttcc atgcctgcgt ctgaaaaaca gacggaaaac aagcggcctg atggcaaatt
      541 cgccgaaggt aacaagctcg ggcggcgctt ccggcccggc cagtccggca acccgaaggg
      601 gcggcccaag tcaattacgc tctccgaagc ctaccgcaaa ctactcgccc aagtggacga
      661 acgtccccgg cgtgagtgac cgagacttgg ggccgtcgaa gttgtcggga attaaactca
      721 ctgacgagca gcgggagaag gccgccgaga ttcgccgggg gcgggccggc tgataaacgg
      781 acggtgaaca aacggcaagg cgatgatttg acgagagcgt tacctgagat tgaagcctat
      841 ccggttccgt caactccagc gggcggcgcg caaactcgcg aacatccagc aggagagggc
      901 gaagatggcg cgggcgcgtc aagactctga agccagtcaa caaggtcgcc taacgcttaa
      961 atcaagtccc cgccctcgac ccggctgata acctatggca aagaagagta tgaaagacgc
     1021 cctcgggggg tttgaacgcg cggcgggtga tgccgggcgt taggcggctg gttgagtggt
     1081 aaagatacag gaactgaata tgtcataact cggatgtgaa tgcggtggca cgatctctga
     1141 tacaacagat aatatccctg agcttgaagg gaacgtggcg gagagggtag ggtttagaac
     1201 ccgcgtcggc ctttcgacca ctccggtttt ctgacgcggt gtcagtggag ctgtgcccgc
     1261 gtgttcaatc ttcttcgggc ggcgatggga ttatattctc ggtgagtttg tgtgataatc
     1321 cgcccatgcc tgatcacagt gtaagcccgc ctccgtcacc atcgagcccg ctaaagcctc
     1381 ccttgaaagt attcggcggt tacgagattg agggagagct cgggcgcggc ggaatgggtg
     1441 tggtttatct ggcgcgccag atcgagctga accgccgcgt cgcgctcaag atgttaaccg
     1501 gccatcacgg gccggatgaa ttgcagcggt ttctcgagga agcagagacg gcggccgggc
     1561 taaatcacac caacatcgcg cacatttacg aaatcggaga gcacgacggc gcgccgttct
     1621 tctcgatgga atttgtggag gccggctcgc tcgcggatcg tctgcgcaaa gagctgccct
     1681 cgccggagga cgccgcgcag ttgctggtca acgtcgcacg cgcgctgcac ttcgcccatc
     1741 aaaacggcgt ggtgcatcgc gacatgaagc cggccaacgt gttgctcgat cctgacggcg
     1801 tcccgaaggt cactgacttc ggaatcgcga agcgtcttaa agccgacgct aacctgaccc
     1861 gttccggcgt cgtgatcggc acgccgacct acatggcgcc ggagcaggcg aaggggaaca
     1921 gccggcacgt cgggccggcg gccgacgttt attcgttggg cgcgatcctc tacgaaatgc
     1981 tcgcagggcg cccgcctttc ctgccggaag acagcgagac agcaatcacc gtgcgcgtcc
     2041 ttaccgagga cccggtctcg cccgcatacc atcggcccga gattccccgc gacctcgaaa
     2101 ccatctgcat gaagtgcctc gggaaagaac cgcgcgaccg ttacgcgagc gccgcggccc
     2161 tcgccgaaga cctgcggcgc tatctcgacg acgaaccgat tctcgccaag ccgccgaaca
     2221 ccgtttccag cagcataaaa tgggtgcggc gaaatccatg gaaatttgtc gcgacggcga
     2281 ccacactgtt gctcgccgtc gccggtctgg cacttctcgc gcggtgggag ctttatcagc
     2341 gtccgcgtct ggagtatgcg gcgcaggtgg cgtgggtcaa cgggtgcctg gagccgctgg
     2401 agaagatcgg cgaggagaac gcttcacagc gcgcagccta cttgcgattg acgcggcgcg
     2461 ggcggttcgg ccccatcgtc aaggcggaag tcttgaacgc gcgcggtcac ccggccgtgc
     2521 tgcgtcggat tttgaacccg gagatgattc cgatctacat ggagggcttg gccggggcgc
     2581 aactttatgc ggaggcgcga caggaaacca caaaggtcga attctggttt gacgagcgca
     2641 atcaggcgcg ggaagcgacc ggacgtgatc ggaacggcca ggtgaactgg cgcatgctct
     2701 acgatcatca ggacgcctct ggttccttcg gtcacaccgc gcgggcacgc ttcgttaaca
     2761 tgcgcggctt tgacgccacc agtcgcgacg gcgcgtcgca catggaattc gaacgcgacg
     2821 ccaaaggccg cgacatgaag atcagcttct tcaacgctgc cggcaagccc gccgccaacg
     2881 gggaaggcgt ttacgggtac acgctcgagc gcgacgacgc gggccgcatc gttcggttgg
     2941 taaatctggg gctcgacggc cagcccgcgg cgaatcgcgt gggtatgatc gccttcaaca
     3001 tgagctgggg cagggaggat cgattagaag tccgcgacgc gaagggacaa ccgtccgtgt
     3061 ggaatggcgt cgccgcggtc gtgagggaga acgatggctc gggcaacccc gtgcgcgtca
     3121 ccaatctcga ccctgaggga aagcccgtga gcgccgcgga gacggaatgg tcggtgcagg
     3181 aaatgaagcg caacgaacgt ggcgaactga cgcaacgcac ctatttcaaa gccgacacgg
     3241 acggcgcgct caaacagatc agccaggtca acgtcactta cgacgatttt ggtcacccgg
     3301 cggatgttca attcgccggc gcgacctcat ggcgaagcgc cttgcgccat gacgcgaatg
     3361 gcaacgtcat cgaggaaaaa cttctcgacg ccgagggcaa gccgcgcgcg ggtgataaag
     3421 gttatgcgat caaacgcttc acctacacgt ctggatcgag cggcgttcgg atcgagcaaa
     3481 catatttcga tgcggccggg gaaaaaactt acagcacggg tgcgggcggg tatcaccgat
     3541 tgattgacga attcgatgcc accggggccc tgcgtcggca gacgatggac gagcacgatc
     3601 cggcgcggtt caaatattac cggtatgtca gtgagccgga atacgatcct caagggcgtc
     3661 tccgtcacaa cgtttctcgc tttgaggacg cccaggggaa cctggccacc gacgccgatc
     3721 tgctcttcac cgcgaccgaa gatttttacg atgagaacgg ccgcctcacg accgaatgga
     3781 aaattggctg cgccctggag gattttggcg gcccggtctt gcgcctcgat acggaatggc
     3841 actccaacgg aaaaatgaag cgctgggtga aacagatctg cgatgcgaat cgccaaccgt
     3901 tgtctttcct ttccaacgga aacggtgcgc gtcacgaaga ggagtacgac gcgaaagaac
     3961 agcgggagcg catttacgat accggattcg acgagaagct ggtcggattc agcacgcggg
     4021 aagcaaagtt ttccggcggc aatcttcaaa gcgttaccca cacgcgtagc gacggaacgg
     4081 tgctcgattc ggtgcgcgtc atcatcaccg ccgtcgcgcc gccggaaggc caaccgaaat
     4141 ccgcggaact gagagcgggc gatcagctta tcgcctccaa cggcaaatcg gtgacgagcg
     4201 cctacgcatg ggtaaccgga ggcttcgccg ggggctggat agaagtcttg cgtgaaggcc
     4261 gacgcattcg catcgatggt ttcggccccg gtgtgcttgg agttgaccta caggatcgcg
     4321 cgcccgcagg aaagcaataa ttacccaaaa gttccttgac gaatttgcac ggctttgcga
     4381 ataatgcgct tcgcactgct ttgcaaatca tgctgtacgt gaaagggagc gatggctgac
     4441 tcaccaacta cacccccggg taggaaggcg ccggtcaaag acgaactcaa tttgcttgaa
     4501 gttttgcgcc ttttaatcga gacagaaaaa gcaaccacta cttccgacct gctgaagtac
     4561 gcttcccagt acggcgctcc cgaagctgaa gaacgactgc gcgttctcga agcggagaac
     4621 atccctctcg atcttgcgtt cgagtcgatc agcgtgcaca tacgcctcct cgcctataaa
     4681 cggaacagcg aattactcgc gggctgccgc ggtcagaaag tagggctcat tctcccactc
     4741 ccgccacatt tgccgaatct atttcttccc gtggcggacg tgacatttct cgaaccgagc
     4801 gagagaacgg ttcacggctc gctgcacgac ttcatggagc gtgcggtgaa aggggcgcgc
     4861 gcctgccgcg cgaaagcgga ggagatgcag gcgctcgtct tcgaagcgtt tcgtgacgac
     4921 gacaatttgt ttgtggaggt agcggtggcc gaagtgctcg agccgaaact ccttccggcc
     4981 ggcatccgcc tgatcgccca ccttcggccg catcgaaatc ccagcgacgt tcagattcaa
     5041 cccgcggttg aggtttcatt catatgaaaa tgccgttgcg ctttctcccc ttgtttggcg
     5101 tgatggccgt tttcttcgct tattgcgcgc ccgtctgggc ggccgggggg gcaccagcgg
     5161 ctggcaccgc tcccgcgcta atcgattcct gggagatgac ctggcccgcc ggcgtgctca
     5221 tgctcgtgct gctgttcggc gcgttcttcg ccatgaccag gtacgaggtg ccgctgccgc
     5281 tggcgatgac cctcgtgagc ctggcgtttc tcgtcatcca atgggaaagc gcgccggtaa
     5341 tactccgcga aggcttcgcg cattacgcgg acatcacggt gttatttacc gccgtcgcca
     5401 ttccggcgca catgattgag cgctcgcagg gattcaaatg ggttgccgca tggctgggcc
     5461 atcaactggg caaggtcagg ctgcgccacc cgcgcctcac gacgccgctg ctcgtcggca
     5521 tcctgctctt tgccacttat gtcaccgctg gactcatgca caacgtcacc tcgattctga
     5581 tcatgacgcc ggtaatcatt cggctgtgcg cgtcgtatca cgtgccgtcg cgctggattc
     5641 tgagcgcggc actcatcgcc agtaatctcg gcgggttttc cacgcgttgg ggcgacacgc
     5701 ccaacatcat cgaatcggcg acgtggggcc tgacggccgg cgatttcatg cgcgaggtgc
     5761 tgcccgcgaa cctgatcgtg atggcggcgc tcatcatcgt ggctacgatc ctcacccaac
     5821 gcggaatcag ccaacggagc cgacaggccg gtgtcaccca ggttcctttc tatactttgc
     5881 aggttgcgca gaccgcggcg gactggaaaa aggcaaaagc cgacctcgcc attgatcgcc
     5941 gcctgctcac gatcggtctc gtgtcgctcg tctttttcat cgtgttgcat gtgatcttca
     6001 ccaagctcgc catcgccatc ggtgcgatca caatcgtcac cgcagtttta ctcgagcgac
     6061 caaaagaacg ctatcacacg ctcacctcgc tgggctttga cgtttatctc gccttcgccg
     6121 ccatcttcgt tctcgccggc tgcgtctcgc attcgtggat cggcagtacg ctttacaacc
     6181 aactcatcgc cgcgaacgcc gcgccctggg tcatcgccgt caccggctat ttcggcacca
     6241 ccttcacgga ggcagcttca tgggccacgg ccgtttcggt tcccatccag caactcgacc
     6301 catcgcacgc cgccgcctgg gcgctcggcg gcggcatctg cgccggcagc agttcgctgg
     6361 tcacggccgc gtcggcgggg atcattctgg cggaggaaag tcagcgcttc aaggaccccg
     6421 aacacgccat cactttccgc cgttaccttg ctttcggcct gccgttctcg ctctttatgc
     6481 tcgtctttta cagcctctat ttcacgttcg tgcgttaact cgttagtcat ggccgaacag
     6541 cgcccgacag ccgaaccgtc acccttcctg ggcgatctcg ccgtcgtctg gaaacgccgg
     6601 aagcagatct ggcgacttct tccgcgcgcc gataagctcg gcttcgggtc gggcttgctc
     6661 atcatggcgc tcgtcgcctt cctcgagaac cagattccct tgctcctggg gaagtttttt
     6721 gatggcgtca cgaacgttgc ggcccagccg aaggagctga tgcccttcgt cattcgggcg
     6781 ctggcaattc tcaccgcgct ctacataggg aaggagtcgt tgcaacttgt ccggcgctgg
     6841 ctggtaagcc gcaccgccac acgcatcgaa gccaacatga tggtgcgcct ggtcggccat
     6901 ctgctcaaga tcgatctggg cgcgctcgcg cgcgagcgcg tcggatcgct gcacggccgc
     6961 atctcacgca gcatcgaagg tttcgttaaa tttctccgag ttagctttac cgacttcctc
     7021 cccgcggtgc tcaccgcagg ggtggcgctt tactacggcc tcgccactaa ttggaaaatc
     7081 ggcctcatca tggtctgcgt ggtgccgctc tccctgctga ttacgatctg gcaggtcagc
     7141 tcgcagaagg gtattcgcga aagtcttttg cgggcgaaag aaggtctgga cgggacagtg
     7201 gtcgagcagt tgggcggcct cgagtacatc cgcgcggcca acacttatcc gacggaagtg
     7261 gcgcgcatcg acacggccgc ggaagcgcgg cgggcgcgag agatgaaaca cgggttggcc
     7321 atggcgcgat tcgattgggc caaagcgatt aacgaagggc tttttcacgt ggcgatcatc
     7381 ggctacgcca tcgtgctcgc ctcccgcgga gagattgaat atggcaccgt gatcaccttc
     7441 tcactcctct acctcaacat cgcgcggccg ctgaaggaag tgcatcgcat tctcgacgag
     7501 acctactcga gcagccagca agtaagcgtg ctgctcgaaa tgttgaacca gccgcaggac
     7561 caatctttcg aggtcgtcac tgtgcgtgcg cctcggctcg acggcagcat tccgttgctg
     7621 gagtgccagg acctggtcgt tgattacact acgcccgagg gcgcgccgcg gcgcatcctc
     7681 aacggactga cgctggcaat tcgtcagggc cagaccatcg gcatcgccgg tcgctccggc
     7741 tcgggaaaat caacctggct gcgttgcatg ctgcgattga ttcatcccgc gtccggcgaa
     7801 gtcctggtgg gcggcgtacc catcggcgtg ctctcccgcg aagacatcgg gaaaagcatc
     7861 ggctacgtca gccaggtgcc ctttgttttt tcagggaccg tctcggaaaa catcgcctac
     7921 ggctgcggcg cgaccacttc ggacaaaatt caggacgccg cccgccaggc gcacatccac
     7981 gaagaaatcc tgcagatgtc ccagggttat gacaccatgc tcaccgaacg cggcggcaat
     8041 ctttccggcg gccagcgtca gcgcatcgcc ctcgcgcgga tgtttctcaa aaaccccccc
     8101 gtcctcatcc tcgacgaagg aacctccgcg ctcgataata tcagcgagcg gcgtgtccgc
     8161 gccgccatcg accacgcgcg tcaatcgcac accgtcatca tggtcgccca tcgcctaacc
     8221 acccttaatc aaacggactg catcttcgtc ttcgacgaag gccgcgtcgt cgaacagggc
     8281 gccttcgatg accttgtcgc aaagaatggc gtcttcgccg aactcgtgcg gagtgccgag
     8341 gccacctaga gaggaaaccg gataatcaaa tgagcaccaa atcagtcacc ttcgtgtggc
     8401 cgggcgatct ccatctcgaa acggacgacc gccccaatta ccagaccgcc cactggatgg
     8461 cggatgaagt caatgaactg atccggccgg acttcgtcca gttcgccggc gacaacgtgc
     8521 agcacgcccg cgacaaagaa tgggaactct ttaaaaacgt gacgagcaaa ttgaagatgc
     8581 cattccacgc actggtcggc gatcacgacg cgcatcacga tcccggttgc cactcgtatc
     8641 aggcgcaact cggcgcgacc tacgatgcgt tcacggtcgg tggttatcgt ttcatccgtc
     8701 tgaacacgaa tcagtacaag ccggtcggtt tgagcgacga acaggtaatc tggtttcgct
     8761 atgaagtgga cgccgccctc gcgcgcgggg agcgcgtcat cgtttttcag cataattatc
     8821 ctttccaggt gtgggaaaat tacgccgggc cgggcatcga agagtggcgc gagatcatgc
     8881 aaacccggcc ggtcaccgcg cttttctccg ggcacaccca ctacgggcag atggccaacg
     8941 atggtcgcaa tgtttatttc gcgatgcgat caatcggcga tccggaaggt ggagccgccg
     9001 gttacgccat tgtgcatctt gatggggagg acctcgcact cacctatcgc accagagagg
     9061 aacgaggccc aatcgcgctg attacgcacc cgcgccgggt cattctggca acaaagcctg
     9121 cgcatattgt gacaggcccg accgaatgtc acgcgcgagg atggtcggcc acgccgatca
     9181 cctcagcgca ggcgcgcacc gacgaaggag actggagcga gatgcgccag acaggtgaga
     9241 tgacgtggag tttccccatc cccggcgaca cgctggcaaa gggcgagcac accttggaag
     9301 tgcgtctaat cgacgaaaac aaagacgaag ggacagatcg gattactttc gcctgcgact
     9361 tgtcgggccg atacaatcct tacccgatgg tcgagccggt ggtaaaggga acgaaattct
     9421 gctgaaaaca gcacacaccg caccgctccc gctattactt tccttagttg aagtgtgaga
     9481 aaaaagcacg acgccgctgt gctctcttct cacacattca acccacatca ttactcacct
     9541 cggatggacg cgccgctttt gttggctctt taagacgctt cggcttcggc acatccattg
     9601 ccctgtagcc aatcgcagca cattcattgg cggaagccgt gagtctggcc tgccggatgc
     9661 accgttttgt gctccgtacc atgatgagag ggagtaagaa tcatgtctct attcggcaca
     9721 cgccatctcc tggtggctgt cagcaccttt atcatcggta tcggaacttt tgcggctatc
     9781 tgggggatgc cgtgggttct gacgcgcagt gtcacaatta agagtcaggt cgccgcacat
     9841 ctgctcgatg accggggcgc ggttaacggg ctgctgctcg ccagcggcga ccagttgcgt
     9901 ttcagcccgc aaacaggcga agcggtcgcg gcgcaaataa attcgggcga cgaagtaact
     9961 gcgacggggc gcgccggctc aaaatcagat tacgggcgcg aagttcgtgt cgaacagatt
    10021 tccgcgaacg ggcgcaccat catcgaagcc gggtcggggc caaagcatcc acgtaagtca
    10081 cgtgacggga gtatgcctca aggccctggc ggccgacccg ctccgcctgc tgcagccgcg
    10141 caagggggag caaagacgga accgaatgtc gcaccgagtg gcgatgcaga cgctaatcag
    10201 acggagaatg ggcagccggc gccgccggag acattcaaag ccaccggcgc gatcaaagac
    10261 catctggtca accgtcacgg cgacgtggac ggcttgatcc tgtcgggcgg agtgcaggtg
    10321 cgtttttcgc cgaaggttgg cgagcttgtg gtagccgccg agcagggggc agacactcag
    10381 gtgagcatcg aaggcacggg cgtgcggaac gagcgcggca cggtgatgcg cccggcgcta
    10441 atcacggtcg gcaaccagac tatcgcactg gggcggtgac cacgacggac agccacgtgg
    10501 actgcgctga cggttgcctc agaatcgaag gccgcgccgg ttgaggcatt gacaggatgg
    10561 cttgtatggc tagcgcggca cgagcacagg atcaaccgga acagaagatg acgacgctgg
    10621 atgcaatcag aaagatgagt ttgcgagcgc ggctggcaat gctggccgcg ttcgcgctcg
    10681 tcgcgctcct cgtcgccctc ttcgtcgcct ggcgtttggc tcgcgcgacg gaagtattcg
    10741 ctctccgcca ggctgactct tcactgcacg cggcggcgcg tgatctggcg cgtgcgtttc
    10801 atgcccatcc gtcaggttat caaaccattg aagaggccgc ggctcggccc gaccgcccgg
    10861 cagcgccccg tgataaaggc ccacgcgaac gcccattgcc gccacatgtg caaacgcttt
    10921 ttgccgcgta tcgggacccg ctctcacgcc tcacggccgc cacgctccat cgtttccctg
    10981 agacggccgg aggcttctac cgggccaccg acggcgcgct ggtcggccaa gccttcccgg
    11041 caaatgctgg aacaggcggc caaatggttt tagcctcgga caggactgat atgatccgcg
    11101 cgctggcaaa gcaggtaaac gaagcgggag cgcccgccac gcgcgcgatg caaacgggga
    11161 gcgaccgcat catcgtcgtc gcctacccgg tgcaagcgga cagctcagag gttgaggaca
    11221 tctcatctcg ggcggatgga gactcatcaa gtcccatcac ggcggcgtgg accatgcaac
    11281 gtctctcgaa cctcacgggc gtctctgaca aagctaatct ggcggcgctc gcggtgctca
    11341 tcttgagtat catcgtggtc agcgggctgg ccctcacgac cgtcagggac ctacgctccg
    11401 gcgtgtcggg catcgagcag ggactggcgg gtctcacgaa tgatctcagt caacaaatcg
    11461 cgccgcctct gacgccggaa ctcgcgcgca ttaccggggc gatcaatcaa ctggccgaca
    11521 cgttgcgcgt caacctcgcg cgccagtccg aactggaaca cgacctgcgg cggagcgaac
    11581 ggctctctgc tctaggtcgc cttatcgcgg gggtcgcgca tgaggtacgc aacccactgg
    11641 cggcgatcaa gctcaaagtc cagatggcgc gccgcgccaa gagtgcgcct gataagttag
    11701 acgatacttt ccgcgtgatc accgaagaga tagatcgact ggatgcgctc gtccgccgcc
    11761 tcctggaatt cagtcggacg cggccacccg aacaaagcaa gctcgatttg tgcgaacttg
    11821 tccggcaccg cgccgcgctc ttcaatgacg tagctgagcg cgccggcgtg aagatcgaaa
    11881 cccgtgtgtc tccggacggc gtgatgatcg aaggcaacac ggagcgtctg gcgcaggtgc
    11941 ttgacaacct gatccagaat gcgctggagg cgatgcccga cggcggacaa ctcgcgatca
    12001 gttgtcaggt cgtcactacc gaagatggcc tcgcagtggc ccgcctcggc gtcgaggaca
    12061 cggggcgcgg catccccccg gaaggtcacg agcgcatctt cgagcctttc tttacggccc
    12121 gcgagacggg cacgggtctc ggtctggcga ttgcgcgcga gatagtagag gcgcacggcg
    12181 ggcgcatcaa ctttgtcagc cgcgccggga gcggcgcgtt ctttctgatc gagttgccac
    12241 aacgggtaca ggctgaggac agtaaacgat tcgatcaacg accgggattt aactcagggg
    12301 gcgcggaatg aagacggtca tcttactcat catctcgaac atttttatga ccatcgcctg
    12361 gtatggtcac ctcaagcaca aagagaggcc gattgtctgg ttgatcttga ttagctggtc
    12421 ggtggcgctc tttgaataca tctttcaggt tccggcgaat cgcatcggct ctcagacata
    12481 cagcgtcacg caattgaaaa tcatgcagga gtgcatcaca ctggtggtct tcacggtgat
    12541 cgcgtatttc ttcttcgacc aaacgctcaa gtggaacaac ttcgtctcct acgcgctcat
    12601 cgtcggggcg gtctacttct cgttcgcaca ttagcaatat gaaagaagac gcggctcaag
    12661 ttctgatcat cgatgacgag acgaacctgc gggagagtct cgccgagttg gttgaaagcg
    12721 agggcttccg cgcggcacaa gcggccaatg gcgaggaggc cgtccgcgtg ttgcgcgccg
    12781 gcgcgaccgt cgccgatgtc gtcttcctcg acatgcggat gccgaagctg gacggactca
    12841 gtgtgctacg cttaattcag gaagagaagt tgaccgacgc gccggtcatc gtcatcagcg
    12901 cttttggcga cagctccaag atgatcgagg cgatgaggct cggcgcctac gactacatca
    12961 ctaagcctct cgatctggat gaaacggtcg ccatcctgcg ccgtgccgcc gagcagcgac
    13021 ggctcaaccg cgaagcggag gcgttgcgcc gagacggttc tacaaacgag agcgctaaag
    13081 aggcggcgtc tagtgaccgg cagttcgaga tgctcggagt gagccgcgcg atgcgcgaca
    13141 ttttcaaaca gatcggacgc atcgcagcga ccgacgccac gcttctcatt acgggtgaat
    13201 cggggacggg caaagagctg gtcgcgcgtg ccgtccaccg acattcggcg cgctcccgcg
    13261 ggccgttcgt cgccgtcaac tgtggcgccc tgcctgaaaa tttgatcgag acggaacttt
    13321 tcggccatga gcgcggcgcg ttcacgggcg cagaacgtca gaagaaaggg cgcttcgaac
    13381 tatcccacac gggtacgctc tttctcgacg aggtcggaga actgagcccg gcggcgcagg
    13441 tgaaactgct gcgcgccgtc gaggcgcgcc gcttcgagcg cgtcgggggc accgagtccg
    13501 tcggcgtaga cgtgcgcgtc atcgccgcga cgaaccgcga cctgaagcac gaggtggaag
    13561 agaagagatt ccgcgaagat ctgttctacc gcctgaacgt catcgaagtg cgattgccgc
    13621 cgctgagaga aaggctcgcc gacgtaccgc aactagccga atcatttctc gaacgcgccg
    13681 tttcacgcca cgcgctcaag actaaaattt tgtcgcccgc cgcgcttcgc gaactgctcg
    13741 cttacgactt tcccggcaac gtgcgcgaac tcgaaaacat ggtcgagcgt gccgccgtca
    13801 cttcaagcgg cgaagtcatt ctgccggaac atctgttcgg aggcgaccac gcgacggcga
    13861 ccatcgcgcc tcaaaacgct gaaagcaaat tgtttgaact tccattccac agggcggtcg
    13921 ccgcgctcga gcgcgagttg atccggcgcg cgctcgaagc cgccggcggt aatcgcgcgg
    13981 gggcggcgcg catgctcgga attaatcgcc gtttgctata ttctaaaatg gatgaacacc
    14041 ggatcactta acgagacgac atagaattat ccggcggtcc gcaggggaag gcagatgagg
    14101 ggcagacgaa tagttcaaac cttgaaagga tttaattggc ggcagtggct ggtgctcgcc
    14161 gcctttctct tcatcatagg atttaccggg ctgcacgctt ttcgcttcgt gcgcgatgcc
    14221 gtttactgga gctatcaccg agatgaggag attcgcgggt ggatgcctgt cggctacgtc
    14281 gcccactctt atcgtgtacc tccccacgtc ctgtatcaag cgcttggtct gccgcacaag
    14341 ccgccggaca agcgcccctt gcgtgagata gcgcgcgcgc agaatcgttc gatggacgaa
    14401 gttaaggcgg tcctgcaaga cgcgattgtt cacgttcggc ctccgtatcc accaccgccg
    14461 cccccgcccc cggataaggg aggcccgccg tgaccgatca acttttagga ttgctctcga
    14521 cctacggctt gcccgcgctc ttcgtcattc tcgctgtctc ctctgcgggc gtgcctttcc
    14581 ccatcacgct gctgctcatc gcctccggct ctttcgtcgc gcagggcgag atgggattat
    14641 ggcaagtgct aatgatatgc agcgcgggcg ccatcatcgg cgatcagatc ggctactggc
    14701 taggtcgctc gggcggtcgc aaattcgttc gccgaatcac ggaacggttt ggcggcgcgg
    14761 agatgatcaa gcgggccgaa gcgtttacca aacgatgggg cggggtaggg atttttttga
    14821 gccggtggct ggtgacgccc ctcggcccgt ggctcaacct gacgagcggc atcaccgggt
    14881 actcatggcc tcgttttctt ttctgggatg tcctaggcga agtgctgtgg gtcgtgatct
    14941 atgtgagcct gggcaacatc ttcagcgacc gcgtgggggc gttggccgaa ctgttaggca
    15001 acctgacatg ggtaatagtc ggcgtcatcg tggcggtcat cctggggtgg aaactgcttc
    15061 agtattttcg cagcggcgac gagtcgtcag aggaaacaaa ggctacggcg gtggcttaca
    15121 aaaccgtggt tgaaggttga gttgcaaaaa atgctgtagt cgtaaaagtc tacaattgcc
    15181 atcacgtttt ccagccccaa tgcaatcaca aaaaagcaca taagtagcga atccttggaa
    15241 tgtcaatccg attcctaaac caaagtagaa ggcacctccg gttcagtgat acatgccctg
    15301 agctttacgc atggaacccg tggctcgtga acttcaatgt ccggctgttc cccgtcccgg
    15361 tgcggacgac ctgatcaggg gcgccatccg gcagtgacgc ttcgatctcc agggtgaccg
    15421 tcacctccgc tcccacgtgc cccgcaaggt gagcgatgac ctcgtcggag atccgactcg
    15481 catcccggcc gacgcgcgcc gggtccaggt tcaccgtgcc gtggaaccgc cgcagcctcc
    15541 gctccggtgg cggaggctgc ggcggaggcg gaccggggcc aggacccggg ggctgatccg
    15601 gtcggggtca ggggcaggcg taacttcggc ggccatctgc caccgcgcca ggtccggctt
    15661 caccagcacc cccgcatcgt cggccgtcag cgacacccgg gcagcaagtc cttggcatgc
    15721 ttccactgac cgtcggtcag atcaggcggg tggatttggt taggcatacc caaaacttac
    15781 ctgacccctt ctgacgggca gctacttttc aaacatcttt ctaagctgac tttcgtgcgt
    15841 ccaacatagc cttccatgct ttaccggccc gacgaccctc accgatatcc ataaccttga
    15901 ggaatggggc aggagcgtca aattcagttc tggctaagag gctggcaggg ggatcactgg
    15961 catcattagg tcgaacgaaa gacagacggt aacgatcctt tctaattctg aatttgacgc
    16021 ccccgccaaa gtaataccag ggaaacttta gatcgctcac ttcggagagg gggacatcga
    16081 agatacgtcc ctcctcggta gtgaaggcaa ggcgtccatt agccaacccg agaactccag
    16141 gcacgctacc ggtagcaccg accaggagcc atgccgcagt gcaaagagcg ccatcggtca
    16201 tttgatttcc catcctttca agtgggcacc attggcggaa gccgaacaac acagcgagca
    16261 gcaggccgtg tatgcccaac aaggcgacta tgaggcggca cgcgaggccg ccgataacgc
    16321 cgcctacgcg acttatgaag ccgactccgg ggcgggcggc gatgaccaca tgggacaggc
    16381 cgatgccgaa aagtacaata tggattgggc cgtccacgaa gaaaaacagg ccgactatta
    16441 cgcggacaat gccgcggcct acgctgcgga aggcgacttc gagaatgcgg aaaggtctgc
    16501 ctcgtcggcc gccgcacacc aggagtcggc cgaccatttc ggcgatctag gcgagcacgg
    16561 cggagacctc gccgtttatg atccttcttc agcagtcgcg acggggggac ttacgaatcg
    16621 actttcgatt cgtccgccgc ccaagtggat acggggttcg attcggggct ggatacgagt
    16681 atgaactcgg gagtcgattc ggggacggac gatatttaac gtcagttcgc actgacgttc
    16741 gcaggcaggc cgtgtggcgc cccacgccgc aaatagctct gtcaaatcat tctcgactgt
    16801 ccagttcgac ctttacgcct ggacgccctg cttgcgagtc cggttgaatg agttgagaga
    16861 acccaccgtt tagaaaatca ttctattcgc cttccgtata cacaacttta ccttcgaatc
    16921 tctccgcccg tctgttatag cgtatttcat atttagccag cggcccgcct ctgccacgat
    16981 aatcaatttc atatctccag tagccggcgc ccttcggttt tccactgaac aaataatcca
    17041 cccaacgcct gatctcaact gaacgctttc ctttatcctc aactactaag cgaaccggat
    17101 attgaattac tttgtcttga cgctcactat atccgatcaa agtggtttga agccccatac
    17161 atgccagcgc gtcaaataaa gcgaattcta aagcccgacc atctccattg taatctctca
    17221 gccacatgat ggtaggcttt ccttcacgcc ccgccgggac atctccgaca cggtaatgcg
    17281 agcctgagtg aatttgataa ggagtatcga actcatcaac cccttcgtta tactcctgcc
    17341 tcactgcaag ggtattgatg attctgcgcg tccgtgtatc caccagcgag actcgtgttg
    17401 cgccgctgta ataactcccc ctcgtttctt ccgggcaggt gtaaggctca tcggggaggt
    17461 cacgaggaat cctcctgggg ttgagcaccc acagaatcaa agctctattc gttttgacgg
    17521 gttgagtttc aataattaaa gcgccagtcg gaagcttgta gtcatggctt ttacgttgtg
    17581 ccaaagatgg ggccgctaaa agaattgtgc aagccagaag cgctgttatt ttcatttcac
    17641 actcaatgtc gagagggggc taacgcccgg catcacgcga tgcgcattca aacgtaatta
    17701 ctgctaaatc tagtggatga aggccgcgct cttcgcggtc gggtgcagac cgttgctcgg
    17761 cgcgatgtcg cagcataact gagttaccgg cgattcatcc ttgcgcccga gtcacttgat
    17821 gccaaccttg gtccctccct gcgattgtat agtcaacgta catccacgtg tacgtgaggt
    17881 gtgtcgtcgc ccgattaaca atatctaaga aataatcgaa gctccccatc gatcgcggta
    17941 tttttatctc ccccccatcc gccgtataca cataaaatgt atactctgta tgaataccct
    18001 cgcccgaggt tgtgccgatc atttgcgtcc cagtgacatc ttgccagtgg tagtgtttga
    18061 tttgctcgcc gcgccgcatg ctcttgtttg tcagcgttat gccggtctcc gcatctaaag
    18121 cgatggcgag agtggtggca acataccagc ggagaaagac gcacagcggc accactatgg
    18181 ttccacttag tagaaagagg acaacgatga cgtcggcgag tccaacctcg ccgacggggc
    18241 cgacaactct ctccacgatt gacatcagaa tcgtcgtcag cacgaaccat gcgacaaacc
    18301 ccagaatcaa atgtaagaca gcccataaca gtatgccccc cgcgggcgac tttttgggta
    18361 tgaacacttg tgtcccggtt cccgatatca cttctcacct catttgaaag atgttgtcga
    18421 ttgaatacgc gccgaatgca tagtaggccg caccccgcgg cctactatgt ctggcagtcg
    18481 gcttctaccg cggcccctgg taatctgcca cccaggaggg gggcgcgtct ttcgccgccg
    18541 gccgctaagg catgatgtcg aaatcttccg cgtttgactc ccagtcgagg ggcgaaagca
    18601 cccgaccgat ggcctcgtgg aggccgtcgc ccagcccgtc cgtgaatcag cagacgaaga
    18661 ggcgcgagtc gtagtccggg tcgtcagtct tcacgccgcc gggtgaagtg aacttggtca
    18721 ccgactggaa gctcgggagc aacttcgtcc ccgcagcaac gtcgacgcca gcggcctctc
    18781 aggcggcatg tctcgcgccg cccggtcgcg aaggccttcg cggatgtaac ggcgcgcggt
    18841 ttcggggcga cctcgcggac ccccgctaac gtgcgcgtca gcagtagcga ctccggcgtc
    18901 accgcccgcc aacgtgtaac tgcccgtcct cccgccttct cccatgtcct ccactctcca
    18961 tgttcaaggg catcgcgcag cgggtcatcg ggcggcggtc ccgcgaagtg taaccgagtc
    19021 tcctgagagc gcgagtttac gcgcccgcgt cagggacttg agctggccga gcagacgttc
    19081 ccgaatcctg tcgccgaccc ggctgaagcc gaacaggcgc gccgtctctg agagcagcga
    19141 gtcgacgccg atgccgaccg cgtggccgac gatcagcagc atcgccgcct gaatctcctc
    19201 gggcgggatg tgctcgacgt ccctcagcgt ccagggcacc tgctcgaccg gaactcgcac
    19261 gacaacatcc ctagtgcctg ccggccacag gaagtcgccg gaccgtacgg aaagccggcc
    19321 cgtgtcgcac atgagggcgg cctgttctac cgccctcgtc tcagcaggca cttgggtcgg
    19381 acgaacttcg tctccacaca gggtactata caaactatgc tccggttaa
//
LOCUS       DASVES010000408        17959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1432015, whole genome shotgun sequence.
ACCESSION   DASVES010000408 DASVES010000000
VERSION     DASVES010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432015"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..1108)
                     /locus_tag="VF240_16720"
     CDS             complement(416..1108)
                     /locus_tag="VF240_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176906.1"
                     /translation="MNFELLVSDVRKSFRDPAGGALEVLRGATFTLKAGAALAVVGAS
                     GAGKSTLLHVLSGLEAADSGGISLGGFDIARAHGEELARWRGREVGFVFQFHHLLADL
                     TAEENVALPLVVARARWRVARGEAARMLDAVGLAGRAAHRPGELSGGEQQRVAVARAL
                     VRRPRLVLADEPTGNLDARNAADVGALLLTLCRDVSACLVVATHNEQLARTCDRRLVL
                     RDGRLHAEDPAA"
     gene            complement(1209..2483)
                     /locus_tag="VF240_16725"
     CDS             complement(1209..2483)
                     /locus_tag="VF240_16725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7176907.1"
                     /translation="MPYELFLALRYLRARRGRRVAQLTALAAVAGIAFGVAALIFALS
                     LANGFRDELRDKILRGTAHVTASRADGARIADWRGVAARLRDVEGVAAAEPTTYAGAL
                     LSGPDGAAYALVRGVDSGSAHALGEIRRTLEGGAVEDLFRAEAAGGVGVAETAPVIVG
                     AELAARVGLGRVGAEGWLVSGEESAASPAGFEPRARRVRVVGVFRAGLFEYDSAWVYA
                     PLDAASAGTASTTGGATGASASVISVEAKDVYEAGEVAARLRDALGPGWAVVDWREAN
                     RPLFAALELERRTVALIIALIVVVAALNITTTLALVVVERRADIAVLRTLGAGARSVT
                     ALFVIEGALVGAAGALAGVALGLACVAAANYFGLVRLPPDVYSLASVPLHASARDVSV
                     VALAAFAVSLLAAVYPARRAAHARPAEVLRQG"
     gene            complement(2623..4305)
                     /locus_tag="VF240_16730"
     CDS             complement(2623..4305)
                     /locus_tag="VF240_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176908.1"
                     /translation="MTSPLPITDAAPRRRPAKRRRHHHFTPSIKFYALLTAALFAALV
                     VAHLPVISLPYYWDEAGYFIPAARDLYLTGDPVPTSSPSNAHPPLVPAYVALAWHLFG
                     FHIAVARVSMLLVAALALAGLFRLSERAAEGRAVAWASVLCTALYPVFFAQSSLVHLD
                     VAAAALTLWGLVLYLPPPGATREGEAVTEKTDEGGVTTEKTDEGGADFGGASGLRRRV
                     ACVALFALAALAKETAVLAPLALAGWEVVGLVVRRFRPEAARRVFLELNRPAWHTPLL
                     ALALAPLLAWLLYHQRRTGYFFGNPEYFRYNVEATMNLARVIDAAGWRLRHVLSHMNL
                     WVLTAATAIAMLLRPLSDGGARERRRIDARVQMVFAVVVAAYVVALSIVGGAVLARYM
                     LPVLPLVILVCVSTLRRRVRRWPLVVAIVCAAFVAGLFWRPTFYSIPWEDNLSYRHFV
                     LLHRDAARFIEETYEDPRVFTAWPARDELTNPAYGYTTRPLRVTVGPQDFGRESLEEA
                     RRRKSEYDTALVFSTHGGPSLEELTQAIGGRVRFREERGGQWVAVLEVSSEQ"
     gene            complement(4280..5158)
                     /locus_tag="VF240_16735"
     CDS             complement(4280..5158)
                     /locus_tag="VF240_16735"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176909.1"
                     /translation="MEKRRILIVDSNEELRTLLASVLSELGHEVVATGDRTTALARED
                     LEDFDLIISDLTEDSDAGGGIEIIGELKRKRLMVPVVVSSEETQQNGVIKAFKLGAAN
                     FVRKPYEREELRTIVDKTLGYKLRFVDDLKVLPYVRERVEFELPSDLSLMNGVLHYLI
                     ERVAGLGIIQPERSNLFIALDEAFVNAVKHGNKNDPTKLVRITADLSANEARFQIEDE
                     GDGFDPSAIPDPLDPANLFKASGRGVLLIYNIMDEVKYNDRGNRLTMVKRPDKSLDSE
                     LIESLSPPDDLSAPNH"
     gene            5393..6151
                     /locus_tag="VF240_16740"
     CDS             5393..6151
                     /locus_tag="VF240_16740"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7176910.1"
                     /translation="MFSEPMRAPADPDTDERAHGTDADAPADERVRRAYAFGDLSGHK
                     LKDRLLIRAADLAFYALIRTVGRTVRFRVEGWEYWEAATRDGGLPVYTFWHDRVFLAT
                     YFWRRRRIVVMTSRSFDGEYIARFIRRFGYGAARGSSTRGGVGALVEMVRLVRQGCPT
                     AFTIDGPRGPRHVAKMGALLLAKKTGQSVLPFSVNAERFWEVPSWDRFQIPKPFTRAT
                     VRIAPPIHVPPDADDSALEAKREELQRALDAVAI"
     gene            6358..8376
                     /locus_tag="VF240_16745"
     CDS             6358..8376
                     /locus_tag="VF240_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176911.1"
                     /translation="MSTSHSGLRVLSLILLASASALAQEPAAPARPRQSAAITITATA
                     TERGLRFAAVGPVRQTRLEVYDASGARVFDSDFLPGNVRDWAAPEQGDDRDGAYTCVL
                     TARDLMGRFSVKQAAVLVEGGRAALSLGEAEPSGGVGASRGEAAGAATLSTHDGRDGA
                     VTATAGDLTLRTGDILAGKDEERMRVTAEGRVGVGTDKPEATLDVAGEVRARDGFRFS
                     DGSRLTISDAGALVRLSSDGTPLPGWGTRPETQAGALADTVVDGDLVFTNPGFPNYGR
                     DIRLSDNLGGVRIYGAPSLSASPAGAAIQFFGNGHALFPGQAYVDAGAHNSAAVIFRS
                     APTGGTIAERMRVTSAGNVGIGTNDPQAKLHVNGTVNFTGLRTQATYVPNIIGGNSSN
                     AVTAGILGATIGGGGHPDAANRVTDIFGTVGGGVGNRAGNNAGTTEDAYAATVSGGDT
                     NTASGPSSTVGGGTLNHASGLHSTIPGGSDNRAQGLWSFAAGTYADSQHDGSFVWSDS
                     TRWNIHTYFSSTAPNQFLINATGGVGIGTNAPGARLHVSGGAILLDNNQGLFLKNTSG
                     AQKRALLGDPSNILRVGSGGSQGFDEIRFDVGSFGEAMAINASGNVGVGTISPQRKLH
                     VSDAMRLQPIASPPASPASGDLYFDTSEALCVYVSGAWAKLAGPGACQ"
     gene            8532..9704
                     /locus_tag="VF240_16750"
     CDS             8532..9704
                     /locus_tag="VF240_16750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7176912.1"
                     /translation="MINQERIKNLLLELVQIDSVSRKEREVAERIKQLCEEMGAQVEI
                     DDAGEKVGGNSGNVVARFPGTIPGAETIMMSAHMDTVVPGEGVKPVVEGDIIRSDGTT
                     VLGGDDKSGCAVIIETIRCLQEQNIPHAPIEAVFSICEEVGLLGAKHVDVSKLASKYG
                     LVFDSDDPGFLFTKGPSANHFEFKIYGLESHAGVAPEEGISAIRIAAEAISAMKLGRI
                     DKETTANIGVIQGGEATNITTNFVLLRGEARSLDEEKLNKQTEHMVKCLEAAAAKYEV
                     TVAGVTTKGRVEAHVNREYYAMDVPETSRVVQLVYEAAKRKGLKVEAVASGGGCDANI
                     FNKRGIECANFGTGMRAIHTVKEWLDVKDMYASAEMTLEVMRLNGELALKAAGQNA"
     gene            9895..11085
                     /locus_tag="VF240_16755"
     CDS             9895..11085
                     /locus_tag="VF240_16755"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEX7176913.1"
                     /translation="MQRLFTPLLLLLAVTLFVGGAAARVQKPEPRKLFPVEGVGGGTS
                     FIDRTGRVVIAPDGIDFTDVVKDGQYRLPMPPSWRDADGDGDYPGRRRRARIEDFSEG
                     LASFSIDTRPGSHRVRLAYGFVDETGRVVIAPTFNRAPGDFHDGRALVKGEDGRRGYI
                     DRTGKTVVAPVYKFAWPFSEGLALVSPDGESYGFIDPDGGVRIPLRFVGGGHFSEGLA
                     RVWVGDYQAAYIDRAGRVLFRLSEGDHGGEFREGLAMLRVGGRDGKCGFIDRAGRTVI
                     PVEFDDARDFSEGKALVKRGDSWGFIDRSGRFVIPPIYAYGSSFSEGLAAVAVAREGT
                     EAGWGYIDHAGNVRIPLEYDHAAPFSGGLAAVDRTMDGLSLGVDAYIDQQGRVVWEKP
                     PRGW"
     gene            11179..12894
                     /locus_tag="VF240_16760"
     CDS             11179..12894
                     /locus_tag="VF240_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176914.1"
                     /translation="MPTPSVERLLARLDELKRPSGAAGRKRLRTLLSQVARRRLRDAD
                     SLIRLHEILVFMRAYPQGAGLLRQTESILKTFSRRIAEARDAGADLSPLAEPEVSGIA
                     GTEFSAIFSYEVVRWLAGAHPSRVEIDWDDYEDRAHLNVVWPRLLPLFEEDAYVDTRA
                     PFLKWLRAARRRGETDLQWLMRGFDRLPVSDKERAELFDPLKIWVRWRLGDTRAARTH
                     ARRSVRKIFFHTEPLLGRRDVSLARELEGPPLAVEKLTRADAERMLAVARDAMAVRYR
                     ELHGFTYPDTRSVVRADAGRGTEIYAWGVPPARRLPLLAYLSMMVFKNGVPAAYAEGL
                     TLFERTEIGFNLFYTFRDGESAWTYARVMRLLRQLHGVETFSVEPYQLGSSGNEEGIE
                     SGAFWFYRKLGFRPTEAEPSKLLRAEERRLAARPAYRTPPATLRRLASGYALYETPRA
                     EAGAWDDFRVRHIGLAVQRRTRDRFGGDPERTRAVSAAQVARALGVSTDAWTDDERRA
                     FERLSLALALIPDLARWTPDERRLVVRIIRTKAGPDETKYARLLHGHTRLRRALIELG
                     STVKH"
     gene            12982..13929
                     /gene="era"
                     /locus_tag="VF240_16765"
     CDS             12982..13929
                     /gene="era"
                     /locus_tag="VF240_16765"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX7176915.1"
                     /translation="MQEEESTPEEESAAPAGREDYRSGYVALVGRPNAGKSTLLNRLV
                     GEKIAAVSNKPQTTRHQIRGVITRPEGQLVFVDTPGVHQPGYLLNRRMMASVHDALEG
                     VDLICLIRDASVSTGNGDRFVLDLVKQTGKPALLLLNKTDKLSDKTALLPLIEWYSAE
                     HEWAGVVPLSARKGDAVAALVSEVVKHLPTGAPIFGEDELTDQPLRSLVSEIVREKIL
                     QTTGDEIPYVTAVVTERYDESEPGLTRIFCAIYVERNSQKRIVIGKGGERLKEVGTRA
                     RRDIERLTQTRVYLELFVKVEQDWRNSERALNDLGINER"
     gene            14011..14901
                     /locus_tag="VF240_16770"
     CDS             14011..14901
                     /locus_tag="VF240_16770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7176916.1"
                     /translation="MNWYALDLTCEAEAREAAEYALMEAGALGTESSFGAGPDGRAVE
                     GPFARVTGYFDDAPQIERVRAALLDALHVYGLASTAVRDIGAREVPERDWLAEWKRGW
                     QPVEVGRSFLVAPPWSEVGAEPGRVVVRIEPGMAFGTGTHETTRLCLVAVEQHFRGGR
                     LLDVGTGTGVLAIAAALLRPDSVAEACDTDPSAVTIAHENAMLNGVAERINFRVGTVD
                     DSTAPADFVCANLTADVILPLLPALRDAARGGLLLLSGILDTQAEAVTTRLRELGLEN
                     YEITTDGEWVAITIQVISDK"
     gene            14964..15770
                     /locus_tag="VF240_16775"
     CDS             14964..15770
                     /locus_tag="VF240_16775"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX7176917.1"
                     /translation="MSLRRFYAPSDAFAPSGDGAAREVVLSSEESRHLRDVLRLRAGD
                     EATVFDGEGREFACVVREPGGGRGSRGEARGGAAAVLDVRGPVEPPRPESPLALTLAV
                     ALLKGEKFDLVVQKATELGVRAVVPVITRRADVRLRDARDAGARLERWRRLALEAAKQ
                     SGRARVPAVAEPVEFSEFVATAASEDTRRLMFSERDGASLAEALGKADAASMAVTALV
                     GPEGGWESEEIDAAREAGWRVVTLGGRVLRAETAAIAVAALLQHLCGDLR"
     gene            16024..16299
                     /locus_tag="VF240_16780"
     CDS             16024..16299
                     /locus_tag="VF240_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176918.1"
                     /translation="MSFDKDDVVGRARVDLAKRLGVAEGEVEEESVEEAEFPDMALGA
                     PQGDEMSGMMITPGWRIRLKAGARSYEYRADERQLRLCDFKGRNYRI"
     gene            16419..17864
                     /locus_tag="VF240_16785"
     CDS             16419..17864
                     /locus_tag="VF240_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7176919.1"
                     /translation="MMNRQNFVWHASLALMLVAVAAFIGQVRHWQTHTPPPFVEAPRA
                     EFPIHDREVPDAHREARDILVRFRAGVSDERIAEITRERNDAVDDQIESVEGLFEIDD
                     LDDVDPQEVVAEYAALPEVEYAEANEAIELDPVERFSIPAEQQASEAAAPGAGENLPN
                     DPMLGDQWALVNRGLRNGKERADIGAALAWAKTQGSERVVVAVLDSGVDYTHPDLMEN
                     MWLRPGDMPAYMDEHLGAVDDLRGFNAVDNLRDPMDDNGHGTHCAGIVGAVGNNGEGV
                     AGVNWKVEIMPLKFMSSTGTGTTKDAIEAINYVIDRKRAGVNVRVISASWGSTLRSKA
                     LEDVIRKAGKEGILFIAASGNSTADTDRRPHFPSSYNLPNVISIAALTRKDELAGFSN
                     FGVKSVHLAAPGAEILSTWLNGQYREASGTSMATPVVSGVAALILSVDNDLSAEELRS
                     RLLDSVDKLDVLGGKVSSGGRVNAARAVGAK"
BASE COUNT         3034 a         6265 c         5929 g         2731 t
ORIGIN      
        1 ccgccccgca cagacacgca aacgccagcg cggcgcgcaa aaatctcttg tccatcggtc
       61 ttaactcctt cggaagcttc ggtgtgtgga acccaggcgt gaggccggcg gcggggcgaa
      121 gcgcgcgcgg ccgacccggc gaccgcacac aatttttata acacgcggcc ggggcctgtg
      181 gacagaagtt tgtgacgcga ggcggaaagg caagcggcag taggcagtcg tgttggcgtc
      241 tgtcatgcct gcgtttatag cggcttacag ttgagtggat agtaagtcaa ctcaccgccg
      301 aggcgcagaa ggcgcagaga tcacgccgca gggttgaatc tctccgcgcc cgcttcgcgt
      361 cctgcggcgc ctctgcggtg aatcaatgtc gctttcactc aagtgaacgt cgcgctcatg
      421 cagcggggtc ttcggcgtgg aggcgaccgt cgcgtaagac gaggcggcgg tcgcaggtgc
      481 gcgcgagttg ttcgttgtgc gtggcgacga cgaggcaggc gctcacgtcg cggcagagtg
      541 tgagcaggag cgcgcccacg tcggcggcgt tgcgcgcgtc gagatttccg gtcggctcgt
      601 cggcgagcac gaggcgcggg cggcggacga gtgcgcgcgc gacggccacg cgctgctgct
      661 cgccgccgga aagctcgccc ggccgatgtg cggcgcgccc cgcgaggccc acggcgtcga
      721 gcatccgcgc cgcctcgcca cgcgccacgc gccaacgcgc gcgcgccacc acgagcggca
      781 gcgccacgtt ctcctccgcc gtcaggtcgg ccagcaggtg gtggaactga aacacgaacc
      841 cgacctcccg gccgcgccaa cgcgccaact cctctccgtg agcgcgcgcg atgtcgaacc
      901 cgcccaaact gatgccgccc gagtccgcgg cctcaagccc cgagagcacg tgcagcagcg
      961 tggacttccc cgcccccgac gcgcccacga ccgccagcgc cgcgcccgcc ttcaacgtga
     1021 atgtcgcgcc gcgcaagacc tccagcgccc cgcccgccgg gtcgcgaaaa gactttcgca
     1081 cgtcgctcac caggagttca aagttcaaag ttccaggttc aaagtcagga cgcccgcccg
     1141 tcgccgtctc cgcacccgcc gactcttcca actttgaact ttgaactttg aactttgaac
     1201 tcactttttc atccttgccg cagcacctcg gccgggcgcg cgtgcgccgc gcggcgcgcc
     1261 gggtagacag ccgcgagcag gctcacggcg aacgcggcga gcgccacgac cgacacgtcg
     1321 cgcgcgctcg cgtggagcgg gacggaggcc aatgagtaga catcgggcgg cagacgcacc
     1381 agcccgaagt agttcgcggc ggcgacgcac gccaagccca acgcgactcc cgcgagcgcg
     1441 cccgccgccc cgacgagcgc cccctcgatg acgaacaggg ccgtgacgct gcgcgcgccc
     1501 gccccgagcg tccgcagcac cgcgatgtcc gcgcgccgct cgacgacgac gagcgcaagc
     1561 gtcgtcgtga tgttgagcgc ggccaccacg acgatgagcg cgatgatgag cgccacggtg
     1621 cgccgttcga gttcgagcgc ggcgaacagc ggccgattag cctcgcgcca gtccacgacc
     1681 gcccatcccg gcccgagcgc gtcccgcaga cgagcagcca cctcgcccgc ctcgtaaacg
     1741 tctttcgcct cgacgctgat gaccgacgcg ctcgcacccg tcgcgccgcc cgtcgtgctc
     1801 gccgtgccgg ccgacgccgc gtcgagcggc gcatagaccc atgccgagtc gtactcgaac
     1861 agtcccgcgc ggaagacgcc gacgacacgc acgcgacgcg cacgcggctc gaaccccgcg
     1921 ggcgacgccg ccgactcctc gcccgagacg agccagccct ccgcgcccac acgacccagg
     1981 cccacacgcg ccgccagctc cgcgccgacg atcacgggcg ccgtctccgc aacgcccacg
     2041 ccgcccgccg cctccgcgcg aaataaatct tcaacagcgc cgccctcaag cgtccgccgg
     2101 atttctccga gcgcgtgcgc cgaccccgaa tcgacgccgc gcacgagcgc gtaggccgcg
     2161 ccgtcggggc cgctcaggag cgcccccgcg taggtcgtcg gctcggccgc ggccacgcct
     2221 tccacgtcgc gcaggcgcgc ggccacgccg cgccagtccg cgatccgggc gccgtcggcg
     2281 cgcgacgccg tgacgtgcgc cgtgccccgc aggattttgt cgcgcagctc gtcccgaaag
     2341 ccgttcgcca gactgagcgc aaagattagc gccgccacgc cgaacgcgat gccggcgacg
     2401 gccgccagcg ccgtgagttg cgccacgcgc cgcccacggc gcgcgcgcaa gtatctgagc
     2461 gcgagaaaga gttcgtaagg catagaattc gagtgggcag taagcagtaa gcagtaagca
     2521 gtaagcagtg agcagtttca gccgtcaccg ctcacgcgcg ttctttcagg tgcgactacc
     2581 attctactgc tttccgctca ccgctcacct actgctcact gctcactgct cactgctcac
     2641 ttccaacact gccacccact gcccgccgcg ctcctcgcgg aagcggacgc gcccgccaat
     2701 tgcctgtgtc aactcttcga gcgacgggcc gccgtgcgtc gagaagacga gcgccgtgtc
     2761 gtattcggat tttcgccgcc gagcttcttc cagactctcg cgtccgaagt cctgtggccc
     2821 gacggtgacg cgcaggggcc gcgtcgtgta gccgtaggca gggttcgtta gctcgtcgcg
     2881 cgcgggccag gcggtgaaga cgcgcgggtc ttcgtaagtc tcctcaatga agcgggccgc
     2941 gtccctgtgc agcaggacga agtggcggta ggagaggttg tcttcccacg gtatcgagta
     3001 gaaggtgggc ctccagaaga ggccggcgac gaaggccgcg cagacgattg caacgacgag
     3061 cggccagcga cgcacgcgcc gccgcagcgt cgagacgcag acgaggatga cgagcggcaa
     3121 gacgggcagc atgtagcgcg ccagcaccgc cccgccgacg attgacagcg cgacgacgta
     3181 ggccgcgacg acgacggcga agaccatctg cacgcgcgcg tcgatgcgcc ggcgctcgcg
     3241 cgccccgcca tcgctcagcg ggcgcaggag catcgcgatg gccgtcgcgg cggtcagcac
     3301 ccacagattc atgtggctga ggacgtgtcg gagtcgccag ccggccgcgt cgatgacgcg
     3361 cgcgaggttc atcgtcgctt cgacgttgta gcgaaagtat tccgggttgc cgaagaagta
     3421 gccggtgcgc cgctggtgat agagcagcca cgcgagcagc ggcgcgagcg ccagcgcgag
     3481 cagcggcgtg tgccacgcgg ggcggttcaa ttccagaaag acgcgccgcg ctgcctccgg
     3541 tctgaacctc ctgacgacga gaccgacgac ttcccaaccg gccagcgcca gcggcgcgag
     3601 cacggccgtc tccttcgcca gcgccgccag cgcgaacagc gcgacacaag ccacgcgccg
     3661 tcgcagaccc gacgccccgc cgaaatcagc gccgccttca tccgtcttct ccgttgtaac
     3721 gccgccctcg tccgtcttct ccgtcaccgc ctcgccctcc ctcgtcgctc ccggtggcgg
     3781 caggtagagg acgagtcccc agagcgtgag cgccgccgcg gctacgtcga ggtggacgag
     3841 cgaactctgc gcgaagaaga ccgggtagag cgccgtgcac aggacgctcg cccacgccac
     3901 cgcgcggccc tcggcggcgc gctcggacag gcggaagagg ccggcgagcg ccagcgccgc
     3961 gacgaggagc atgctcacac gcgcgaccgc gatgtggaag ccgaacaagt gccacgcgag
     4021 ggcgacgtag gccggcacaa gcggcgggtg tgcgttcgag ggcgaggagg tgggcacggg
     4081 gtcgcccgtc aggtagaggt cgcgcgcggc cgggatgaag tagccggcct cgtcccagta
     4141 gtagggcaga gagatgacgg gcagatgagc gacgacgagt gcggcgaaga gggcggccgt
     4201 gagcagcgcg tagaatttta tcgagggcgt gaagtgatgg tggcggcggc gcttcgcggg
     4261 gcgacgacgg ggcgcggcgt cagtgattgg gagcggagag gtcatcgggg ggcgagagcg
     4321 attcgatgag ttcggagtcg agcgacttgt cggggcgctt gaccatcgtc agccggttgc
     4381 cgcgatcatt gtacttgacc tcgtccatga tgttgtaaat caggagcacg ccgcggcccg
     4441 aagccttgaa gagattggcc gggtcgagcg ggtcggggat ggcgctgggg tcgaagccgt
     4501 cgccctcgtc ctcgatctgg aagcgcgcct cgttcgccga gagatcggcc gtgatgcgga
     4561 cgagcttggt cgggtcgttt ttgttgccgt gcttgacggc gttgacgaac gcctcgtcta
     4621 aggcgatgaa gaggttcgag cgttcgggct ggatgatgcc taagccggcg acgcgctcga
     4681 tgaggtagtg gagcacgccg ttcatcaacg agaggtcgct cggcagctcg aactccacgc
     4741 gctcgcgcac gtagggcagc accttgaggt cgtccacgaa gcggagcttg tagcccaagg
     4801 tcttatcgac gatggtgcgc agctcctcgc gctcgtaggg cttgcggacg aagttggcgg
     4861 cgccgagctt gaaggctttg atgacgccgt tctgctgcgt ctcctcggag gagacgacga
     4921 cgggcaccat gagacgcttg cgtttgagtt cgccgatgat ctcgatgccg ccgccggcgt
     4981 cggagtcttc ggtgaggtca gagatgatga ggtcgaagtc ttcgaggtct tcgcgggcca
     5041 gggcggtcgt gcggtctccg gtggcgacga cctcgtggcc cagctcgctg agcacgctcg
     5101 cgaggagcgt ccgcagctct tcgttgctgt ccacaatgag gatgcgcctt ttctccaacc
     5161 gacctctctt cccgctaagg ctttatatga tggggagggc acgactttcg ggcaacgcgc
     5221 gcttgtgcga gtgaagacac acgctttccg cctacgctgt acgaagccgc acagggcagg
     5281 gtttccacta tatcgcacaa aaaacggcgg aaggccagcc gcgccggaga cgtgcccggc
     5341 cccggccatc cactgaagcc gagaatcatt tctgttagac tcgaaacgcc cgatgttctc
     5401 cgaacccatg cgagcgcccg ccgacccaga cacagacgag cgcgcccacg gcaccgacgc
     5461 cgacgcgccg gccgacgagc gcgtgcgccg cgcctacgcc ttcggcgacc tctccggcca
     5521 taagctcaaa gaccgcctgc tgatacgcgc ggccgatctc gccttctacg cgctcatccg
     5581 gacggtcggc cgaacggtgc gcttccgcgt cgaaggctgg gagtactggg aggccgccac
     5641 gcgtgacggg ggcttgcccg tctacacctt ctggcacgac cgcgtctttc tcgccaccta
     5701 cttctggcgc cgacgccgca tcgtcgtgat gacctcgcgg agcttcgacg gcgaatacat
     5761 cgcgcgcttc atccgccgct tcggctacgg cgcggcgcgc ggctcttcca cccgcggcgg
     5821 cgtcggcgcg ctcgtcgaga tggtcagact cgtccgtcag ggctgcccca cggccttcac
     5881 catcgacggc ccgcgcggcc cccgccacgt cgccaagatg ggcgcgctcc tcctcgccaa
     5941 gaagaccggc cagagcgtgc tgcccttctc cgtcaacgcc gagcggttct gggaagtccc
     6001 cagttgggat cgttttcaaa tcccgaagcc cttcacgcgc gcgacggtgc gtatcgcccc
     6061 ccccatccac gtcccacccg acgccgacga ctccgccctc gaagccaagc gcgaagaact
     6121 ccaacgcgcc ctcgacgccg tcgccatctg aggcgataca gcgcgaaaag cattgacttg
     6181 aggtgcaggc gcgggcaata tcctgagcca cgtcacgctt acgagccacg ggtttaagat
     6241 accgccgcca cgccgctttt tcgccagatg ttttgccgtt tccaaagctg atccccgttt
     6301 tatcatttac tcgatgacgc tcaaccctcg acgacttccg ctgaaggagt ttctcccatg
     6361 tctacttccc actcagggct gcgtgttctt tccctgatcc ttttggcctc ggcctctgct
     6421 ttggcgcaag agccggcagc ccccgcccgg ccccgtcaga gcgccgccat cacgatcacg
     6481 gccacggcca ccgagcgggg cctgcgcttc gccgccgtcg gccccgtcag acagacccgc
     6541 ctcgaggtct acgacgcctc cggcgcacgg gtcttcgact cggacttcct gcccggcaac
     6601 gtccgcgact gggcggcccc tgagcaggga gacgaccgcg acggcgctta cacctgcgtg
     6661 ctgacggcgc gcgatctcat ggggcgcttc agcgtcaagc aggccgcggt gctggtcgag
     6721 ggcgggcgcg cggcactgtc actgggcgag gccgagcctt cgggcggggt tggggcgagc
     6781 cggggtgagg ccgcaggggc ggcgaccctc agcacgcacg acgggcgaga cggggccgtg
     6841 acggccactg cgggcgacct cacgctgcgc accggcgaca tcctcgcggg gaaagatgag
     6901 gagcggatgc gcgtcaccgc cgaggggcgg gtgggtgtcg gcaccgacaa gcccgaggcg
     6961 acattagacg tggccggcga ggtgcgcgcc cgcgacggct tccgcttctc cgacggcagc
     7021 cgcctgacga tcagtgatgc cggggcgctc gtgcgcctgt cctcagacgg cacgccgctg
     7081 ccgggctggg gcactcggcc cgagacgcag gccggcgcgc tcgccgacac ggtcgtcgac
     7141 ggcgacttgg tatttactaa ccccggcttc ccgaactacg gcagagacat ccggctctcg
     7201 gacaacctgg gcggggtgag aatctatggt gcgccctcgc tgagtgccag cccggccggt
     7261 gcggccatcc agttcttcgg taacgggcac gcgctgtttc cgggtcaggc gtatgtggac
     7321 gcgggggcgc acaactccgc ggcggtgatc ttccgctcgg ccccgacggg tgggaccatc
     7381 gccgagcgga tgcgggtcac ctccgccggc aacgtcggca tcgggacgaa tgacccgcag
     7441 gccaagctgc acgtcaacgg cacggtcaac ttcacggggc tgcggacgca ggcgacctat
     7501 gtccccaaca tcatcggcgg caacagcagc aacgcggtga cggcgggcat cttgggcgcc
     7561 accatcggcg gtgggggaca ccccgatgcc gccaaccgcg tcacggacat cttcggcacg
     7621 gtgggcgggg gcgtggggaa tagggcgggc aacaatgcgg gcacgacgga agacgcgtat
     7681 gccgctacgg tcagcggggg cgataccaac accgccagcg gccctagttc caccgtcggc
     7741 gggggtacgt taaaccacgc cagcggcctt cattccacta tacccggcgg gtccgacaac
     7801 agggcgcagg ggttgtggag cttcgccgcc ggcacgtacg cggactccca gcatgacggc
     7861 tcgttcgtct ggtctgacag caccagatgg aacattcaca cgtacttctc ctcgacggcc
     7921 cccaaccagt tcctcatcaa cgccacgggc ggcgtcggca tcggcaccaa cgcccccggg
     7981 gcgaggctcc acgtcagcgg tggggcgatc ctgctcgaca acaaccaggg cctgttcctc
     8041 aagaatacct ccggcgctca gaagcgcgcc ctgctaggcg acccgagtaa catcctgcgc
     8101 gtcggcagcg gcggctcgca ggggttcgac gaaatacgct tcgatgtcgg ctcctttggt
     8161 gaggcgatgg ccattaacgc cagcggcaac gtcggcgtcg gcacgatctc gccgcagcgc
     8221 aagctccacg tctcggacgc catgcgcctc cagcctatcg ccagtccgcc ggccagcccg
     8281 gcgagcggcg acctctactt cgacacgtcc gaggcgctgt gcgtttacgt cagcggcgcg
     8341 tgggccaagc tggccgggcc gggcgcctgc cagtgagtgc ggcttcagac gccgcccgcc
     8401 ttgtagcccg caggcggcgg cgtgtagtaa attagcgccc aagcaatctg aaaccccgaa
     8461 cctcggattc cgaaacggga ggcggcctgc gcccccgaag tcaggtgccc gacgtttgcc
     8521 ggaggcgact aatgattaat caggagcgca tcaagaacct tctgctcgaa ctcgtccaga
     8581 tcgactcggt ctcgcgcaaa gagcgcgagg tggccgagcg catcaaacaa ctgtgtgaag
     8641 agatgggcgc gcaggtcgag atcgatgacg ctggcgaaaa ggtcgggggc aactccggca
     8701 acgtcgtcgc gcgctttccc ggcaccatcc ccggcgccga gaccatcatg atgtcggcgc
     8761 acatggacac ggtggtgccc ggcgagggcg tcaagcccgt ggtcgagggc gacatcatcc
     8821 gctcggacgg cacgaccgtt ttgggcggcg acgacaagtc gggctgcgcc gtcatcatcg
     8881 agaccatccg ctgtctgcaa gaacaaaata tcccgcacgc gcccatcgaa gccgtcttct
     8941 ccatctgcga agaggtcggg cttttgggcg ccaagcacgt ggacgtgtcg aagcttgcgt
     9001 cgaagtacgg cctcgtcttc gacagcgacg accccggctt cctcttcacc aaaggcccct
     9061 cggccaacca cttcgagttc aaaatctacg gcctcgaatc gcacgccggc gtcgcccccg
     9121 aggaaggcat ctcggccatc cgcatcgccg ccgaggccat cagtgcgatg aagctcggcc
     9181 gcatcgacaa ggagaccaca gccaacatcg gcgtcattca gggcggcgag gccaccaaca
     9241 tcaccaccaa cttcgtcctc ctccgcggcg aggcgcgctc gctcgacgag gagaagctga
     9301 acaagcaaac cgagcacatg gtcaagtgtc tggaagcggc ggccgccaag tatgaggtga
     9361 cggtcgcggg cgtgaccacg aaggggcgcg tcgaggcgca cgtcaaccgc gagtactacg
     9421 cgatggacgt gcccgaaacg tcgcgcgtcg tgcagctcgt ctacgaggcc gcgaagcgca
     9481 agggcctcaa ggtcgaggcc gtggcctcgg gcggcggctg cgacgccaac atcttcaaca
     9541 agcgcggcat cgagtgcgcc aacttcggca cggggatgcg cgccatccac accgtcaagg
     9601 agtggctcga cgtgaaagac atgtacgcct ccgccgagat gacgctcgaa gtcatgcgtc
     9661 tcaacggcga gctggccctc aaggccgcgg ggcagaacgc ctgagccgcg ccacgcaccc
     9721 ggacgcccgc gaggccgcgc cgacatcaat tcaacgtcgg cgcggcctcg ctccggcccg
     9781 tcgcctaatc gcgagtcggc ccgacgcctg ctcgcaaacc cgcggcccct ctcgcgttcc
     9841 aacggcatga cacgtaaaca cgtcgcacgt ggaggccgca gactcaactc cccgatgcag
     9901 agactcttca ccccgcttct ccttctgctc gccgtgacac tcttcgtcgg cggcgccgcc
     9961 gcgcgcgtgc agaagccaga gccgcgcaaa ctcttccccg tcgagggcgt gggcggcggc
    10021 acaagcttca tcgaccgcac gggccgagtg gtcatcgcgc ccgacggcat cgacttcacc
    10081 gacgtcgtca aagacggcca gtaccgcctg cccatgccgc cctcttggcg ggatgccgat
    10141 ggtgatggcg attatcccgg ccgccggcgg cgggcgcgca tcgaagactt ctccgagggc
    10201 cttgccagct tcagcatcga cacgcgcccc ggctcgcacc gggtccgcct cgcctacggc
    10261 ttcgttgacg agaccggcag ggtggtcatc gcgccgacct tcaatcgcgc ccccggcgac
    10321 ttccacgacg gccgcgcgct cgtgaagggc gaagacggca ggcgcggcta catcgaccgc
    10381 acggggaaga ccgtcgtcgc gcccgtctac aagttcgcgt ggcccttctc cgagggtctg
    10441 gccctcgtct cgcccgacgg cgagtcctac ggcttcatcg accccgacgg gggcgtccgc
    10501 atcccgctgc gcttcgtcgg ggggggccac ttctcagagg ggcttgcgcg ggtgtgggtc
    10561 ggcgattatc aggccgccta catcgaccgc gcgggccgag tcctcttccg cctgagcgag
    10621 ggcgaccacg gcggtgagtt tcgggagggg ctggcgatgc ttcgggtcgg cggccgcgat
    10681 ggcaagtgcg gcttcatcga ccgcgcgggg cgcaccgtca tccccgtcga gtttgacgac
    10741 gcgcgcgact tctccgaggg caaggcgctc gtcaagcgcg gcgactcctg gggcttcatc
    10801 gaccgctcag gccgcttcgt catcccgccc atttacgcct acgggagcag cttctccgag
    10861 ggcttagcgg ccgtcgccgt cgcgcgcgag gggacggagg ccggatgggg ctacatcgac
    10921 cacgcgggca acgtccgcat ccctcttgag tacgaccacg ccgcgccatt ctccggcgga
    10981 ctcgccgccg ttgaccgcac gatggacggc ctctcgctcg gcgtggacgc ctacatcgac
    11041 cagcagggcc gcgtcgtctg ggagaagcct ccgcgcggtt ggtgagcctc acgcgcgccg
    11101 cccgcatgag ttgacttgcc cacgggcgcg ggttagaagt cttccgctcg actcagcccc
    11161 gcacgcgtgg gttcacctgt gccgacaccg tcagtcgaac gcctgctcgc ccgcctcgac
    11221 gaactcaagc gcccctccgg cgcggcgggg cggaagcgcc tgagaacgct gctgtcacaa
    11281 gtcgcccgcc gccgcctccg cgacgccgac tcgctcatcc gcctccacga aatcctcgtc
    11341 ttcatgcgcg cctacccgca gggcgcgggg cttctgcgac agaccgagtc gatactcaag
    11401 acgttcagcc ggcgcattgc tgaggcgcgc gacgcggggg ccgacctctc gccgcttgcg
    11461 gagccggagg tgtccggcat cgccggcacg gagttctccg ccatcttcag ctacgaggtc
    11521 gtgcgctggc tcgccggggc gcacccctcc cgagtcgaga tcgactggga cgactacgaa
    11581 gaccgcgcgc acttgaacgt cgtctggcca cgactgctcc cgctgtttga agaagacgct
    11641 tacgtcgata cgcgcgcgcc cttcctcaag tggctgcgcg cggcccgccg tcgcggcgag
    11701 accgacctcc agtggctcat gcgcggcttc gaccgcttgc ccgtctcaga caaagagcgc
    11761 gccgagttgt tcgaccctct caaaatctgg gtgcgctggc ggctcggcga cacgcgcgcc
    11821 gcgcggacgc acgcgcgccg aagcgtccgc aaaatcttct tccacaccga gccgcttctg
    11881 ggacgccgcg acgtgtcgct cgcgcgcgag cttgaagggc cgccgctcgc ggtcgagaaa
    11941 ttaacgcgcg ccgacgccga gaggatgctc gccgtcgcgc gcgacgcgat ggccgtgcgc
    12001 taccgcgagc tgcacggctt cacctatccc gacacccgct cggtcgtgcg cgccgacgcc
    12061 ggccgcggca ccgaaattta cgcgtggggc gtgccgcccg cgcgccgcct gccgctgctc
    12121 gcctatctct ccatgatggt cttcaagaac ggcgtgcccg cggcctacgc cgagggcctc
    12181 acgctgtttg agcgcacgga gatcggcttc aatctcttct acaccttccg cgacggcgag
    12241 tcggcgtgga cttacgcgcg cgtgatgcgt ctgctccggc agctccacgg cgtcgagacc
    12301 ttttccgtcg agccgtatca gctcggcagc tcgggcaacg aggagggcat cgagtcgggc
    12361 gctttctggt tctaccgcaa gctcgggttt cgccccaccg aggccgaacc gtcgaaactg
    12421 ctgcgcgccg aggagcgccg gctcgccgcg cgccccgcct accgcacgcc cccggccacg
    12481 ctccgccgcc tcgcctccgg ctacgcgctc tacgagacgc cgcgcgccga agcgggcgcg
    12541 tgggacgact tccgcgtccg ccacatcggt ctcgccgtcc agcgccgcac acgcgaccgc
    12601 ttcggcggcg accccgagcg cacgcgcgcc gtctccgccg cacaggtcgc ccgcgcactc
    12661 ggcgtcagca ccgacgcttg gacggacgac gagcgccgcg ccttcgaacg tctctccctc
    12721 gcgctcgccc tcatccccga cctcgcgcgc tggactcccg acgagcgccg cctcgttgtc
    12781 cgcatcatcc gcacgaaggc cggccccgac gagacgaagt acgcgcgcct gctccacggc
    12841 cacacgcgtc tgcgccgcgc cctcatcgaa ctcggctcga ccgtcaagca ttaacggccg
    12901 cgcgctcggt cgaactctcc ccggcgttgg cggcgcgggg cggcaggcgt tacacttcgg
    12961 tcttaacaaa gttcactatc gatgcaagaa gaagaatcca cgccggaaga agaatccgcc
    13021 gcgcccgcgg ggcgggaaga ctaccgcagc ggctacgtcg cgctcgtcgg gcggccgaac
    13081 gcgggcaagt ccactctgct caaccggctc gtcggcgaga agatcgcggc cgtctccaac
    13141 aagccgcaga cgacgcgcca tcagattcgc ggcgtcatca cgcgccccga gggacagttg
    13201 gttttcgtgg acacgccggg cgtccaccag ccgggctacc tcctcaaccg ccgcatgatg
    13261 gcctccgtcc acgacgcgct cgagggcgtc gatctcatct gtctcatccg cgacgcttca
    13321 gtctcgaccg gcaacggcga ccgcttcgtc ctcgacctcg tcaagcagac gggcaagccc
    13381 gccctcctgc tcctcaacaa gaccgacaaa ctgagcgaca agaccgcgct cctgccgctc
    13441 atcgagtggt actcggccga gcacgagtgg gccggcgtcg tcccgctctc ggcccgcaag
    13501 ggcgacgccg tcgcggcgct cgtcagtgag gtggtcaaac atctccccac tggcgcgccc
    13561 atctttggcg aagacgaatt gaccgaccaa cccttgcgct ccctcgtctc cgagatcgtc
    13621 cgcgagaaga ttttgcaaac caccggcgac gaaattccct acgtgacggc cgtcgtcacc
    13681 gagcgttacg acgaatccga gccgggactg acgcgcatct tctgcgccat ctacgtcgag
    13741 cgcaattcgc agaagcgcat cgtcatcggc aagggcggcg agcgcctcaa ggaggtcggc
    13801 acccgcgccc gccgcgacat cgaacgcctc acgcagacgc gcgtctatct cgaactcttc
    13861 gtcaaggtcg aacaggactg gcgcaactcc gagcgcgcct taaacgactt gggcatcaac
    13921 gaaaggtgat aagtgataag tgataagtga taagaaaaga cagatgcttt ctcttatcac
    13981 ttatcactca tcacttatca cgcaaagcct atgaactggt acgcgctcga cctgacctgt
    14041 gaagcggagg cgcgcgaggc cgccgagtat gcgttgatgg aggcgggcgc gctgggcacg
    14101 gagagttcct tcggggcggg gccggacggc cgcgccgtcg aaggcccgtt cgcgcgcgtc
    14161 accggctact tcgacgacgc gccgcaaatc gagcgcgtgc gcgccgcgct gctcgacgcg
    14221 ttgcacgtct acggcctcgc ctccacggcc gtgcgcgaca taggcgcgcg cgaggtgccc
    14281 gagcgcgact ggctcgccga gtggaagcgg ggctggcaac ccgtcgaggt cggccgcagc
    14341 ttcctcgtcg cgccgccgtg gagcgaggtg ggggcggagc cgggccgcgt cgtcgtccgc
    14401 atcgagccgg gcatggcctt cggcacgggc acgcacgaga cgacgcgcct gtgcctcgtg
    14461 gccgtcgagc aacatttccg cggcggccgt ctcctcgacg tgggcacggg cacgggcgtg
    14521 ctcgccatcg ccgccgctct gctccgcccc gactcagtcg ctgaagcctg cgacacagac
    14581 ccgtcggccg tgaccatcgc gcacgaaaac gccatgctca acggagtggc cgagcgcatc
    14641 aacttccgcg tcggcaccgt ggacgactcg acggcccccg ccgacttcgt ctgcgccaac
    14701 ctgacggccg acgtgattct cccgctgctc cccgccctcc gcgacgccgc ccgcggcggc
    14761 ctcctcctcc tctccggcat cctcgacaca caggccgaag ccgtcaccac gcgcctgcgc
    14821 gaactggggc tggagaacta cgaaatcacc acggacggcg agtgggtggc aataacgatt
    14881 caagtgataa gtgataagtg ataagtgata agaaaatcag cttccctctt atcacttatc
    14941 actcatcact tatcacttct cctatgtctc tgcgaaggtt ctatgcaccg agtgatgctt
    15001 tcgcgccgtc gggcgatggt gcggcgcggg aggttgtgtt gtcgtcggag gagtcgcggc
    15061 acttgcgcga cgtgctgcgg ttgagggcgg gcgacgaggc gacggtgttc gacggcgagg
    15121 ggcgggagtt cgcgtgcgtg gtgcgcgagc cgggcggcgg gcgcggcagc cgtggggagg
    15181 cgcggggggg cgcggcggcg gtgctcgacg tgcgcggccc cgtggagccg ccgcggcccg
    15241 agtcgccact cgcgctcacg ctcgccgtcg cgctgctcaa gggtgagaaa ttcgacctcg
    15301 tcgtgcagaa ggcgacggaa ctcggcgtgc gcgccgtcgt gcccgtcatc acgcgccgcg
    15361 ctgacgtgcg cctgcgcgac gcgcgcgacg ccggcgcgcg actcgaacgc tggcggcggc
    15421 tggcgctcga agcggccaag cagtcggggc gcgcgcgcgt gccggccgtc gcggagccgg
    15481 tcgagttttc agagttcgtc gcgacggccg cgtcggaaga cacgcggcgc ctgatgttct
    15541 cggagcgcga cggcgcatcg ctcgccgagg cgctgggcaa agcggacgcg gcttcgatgg
    15601 ccgtgacggc gctcgtcggg ccggagggcg gttgggagtc cgaagagatc gacgcggcgc
    15661 gcgaggccgg ctggcgcgtc gtcacactcg gcggccgcgt cctgcgcgcc gaaacggccg
    15721 ccatcgccgt cgcggccctc ttgcaacacc tctgcggaga cctcagatag agccgtcaat
    15781 cgtgaatcgt caatcgtcaa tcgtggttga gccaaggccc gtcttgagcg gccccgctca
    15841 aggcgatttg agcgacaact ccatcgattc acgattcacg attgacgatt cacgagcttt
    15901 tgcctttacg gccgcgcggg agcagaatac gtaaagcgtt gaaagtggtg gaacggcgtc
    15961 gcggccggcg gcgtaattca gtttgttgag aacgcaccta gcggcagggg gataaacttt
    16021 ccgatgtctt tcgacaaaga cgacgtggtc gggcgggcgc gggtcgatct ggcgaagcgg
    16081 ctcggcgtgg ctgagggtga ggtcgaagag gagtcggtcg aggaggcgga gttccccgac
    16141 atggctctgg gcgcgccgca gggcgacgag atgagcggga tgatgatcac gccgggctgg
    16201 cgcatccgcc tcaaggcggg cgcgcgctcc tacgagtacc gcgccgacga acgccaactc
    16261 cgcctctgcg acttcaaggg caggaactac aggatttgag gagctgttag ctcttagcta
    16321 aagacaatag cggcggacgc gccgaagata aaacagtgcg gttgaagacg ctggctgaaa
    16381 gctaacagct aagagctaac agctctccga ggagtgaaat gatgaaccga cagaatttcg
    16441 tctggcacgc gtcgctggcg ttgatgctgg ttgcggtggc cgctttcatc gggcaggttc
    16501 gtcattggca gacgcacacg cccccgccct tcgtcgaggc tccgcgcgcc gagttcccca
    16561 ttcacgaccg cgaggtgccg gacgctcacc gcgaggcccg agacatcctc gtgcgcttcc
    16621 gcgcgggcgt ctccgacgag cgcatcgcgg agatcacgcg cgaacgcaac gacgcggtgg
    16681 acgaccagat agagtcggtc gaaggtctct tcgagatcga cgacctcgac gacgttgacc
    16741 cgcaggaggt cgtcgccgag tacgcggcgc ttcccgaggt cgagtacgcg gaggcgaacg
    16801 aggcgattga actcgacccg gtcgagcgct tcagtatccc ggcggagcag caagcgtcgg
    16861 aagcggccgc gccgggggcg ggcgagaatt tgccgaacga cccgatgctc ggcgaccagt
    16921 gggcgctcgt caaccgcggc ctccgcaacg gcaaggagcg tgccgacatt ggcgcggcgc
    16981 tcgcttgggc gaagacgcag ggcagtgagc gcgtcgtcgt cgccgtcctc gacagcggtg
    17041 tggattacac gcaccccgac ctcatggaga acatgtggct gcgtcccggc gacatgcccg
    17101 cctacatgga cgagcacctc ggcgcggtgg acgacctccg cggcttcaac gccgtcgata
    17161 acctgcgcga cccgatggac gacaacggcc acggcacgca ctgcgcgggt atcgtcggcg
    17221 cggtcggcaa caacggcgag ggcgtggccg gcgtcaactg gaaggtcgag atcatgcccc
    17281 tgaagttcat gtcctccacg ggcacgggga cgaccaagga cgccatcgag gccatcaact
    17341 atgtcatcga ccgcaagcgc gccggagtca acgtccgcgt catcagcgcc agttggggct
    17401 cgacgctccg ctccaaggcg ctcgaagacg taatccgcaa ggccggcaag gagggcatcc
    17461 tctttatcgc cgccagcggc aactccacgg cggacacgga tcgccgcccg cacttccctt
    17521 cgagctacaa cctgcccaac gtcatctcca tcgccgcgct gacacgcaag gacgaactcg
    17581 ccggcttctc caacttcggc gtcaagagcg tccacctcgc cgcgcccggc gcggagattt
    17641 tgagcacgtg gctcaacggc cagtaccgcg aagcctccgg cacttcgatg gcgacgccgg
    17701 tcgtctcggg cgtggcggcg ctcattctct cggtcgataa tgacctttcg gccgaagagc
    17761 tgcgctcgcg tcttctcgac tccgttgaca agctcgacgt gctcggtggc aaggtctcca
    17821 gcggcgggcg cgtcaacgcg gcccgcgccg tcggcgcgaa ataaatcggt caacgagccg
    17881 ccggaggcgg cgcatgggac tatagtggct tgcagttgag tgggtagtag gtcaactcac
    17941 cgcagaggcg cagagggcg
//
LOCUS       DASVES010000409         5505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1466156, whole genome shotgun sequence.
ACCESSION   DASVES010000409 DASVES010000000
VERSION     DASVES010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466156"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..668)
                     /locus_tag="VF240_16790"
     CDS             complement(144..668)
                     /locus_tag="VF240_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7176920.1"
                     /translation="MRRHIFAYPLVLLAACAIFAQAGRERAAEIAPAMHVAPAEHVAG
                     HSFHVALQPETIQWKPFYEGAEIAVLEGDPAAAGSHYVIRIKHRDGLKVPPHWHPFDE
                     HLTVITGTWVMGMGEKYDLTGAQEFGAGAYLVSPKNVPHYALSKGETIVQVHGTGPFQ
                     AVFVNPEDDVFKKK"
     gene            952..2418
                     /locus_tag="VF240_16795"
     CDS             952..2418
                     /locus_tag="VF240_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7176921.1"
                     /translation="MAQELFNSQDWISAADRGDADTVERFLAEGADVDVCTPGSETAL
                     MRAAARGHAPLVRVLLTRGADPNLSRRDGWTALMLAAFFGHAETARELLRRGADIEAT
                     DRNGSTASKWATSRGHLEVARLINDASAAGVSIEKEIEKVAPVVETGKDAKADTLRPI
                     APTPERAPEINAAVTPPILTPTSAARRVEPAGDAREELDEVTIATAVRKSSAAPAAPT
                     HAPTPAQVFMQAHAPAPIPAHTHAPPAASIFERADELRAEMLAVSPEVRREVNELAAR
                     AARRADGGARFWRSAALGMALLAVGAALLAYKFGGAGAPSAGDGARFAATPEASAQPA
                     TAAPIEMQPAVPVTPIASPLPADPASQGVGTVGLAPGTQPSVPTYFPPATTDAATTVE
                     TARPRETYPANQPAGRASGGDPALVNLGQSERTADDAPALPTRRPQDSAASDNTRTEA
                     PATRPARDDSAQRSTRTTRPADEPRPTPTPKGKVVNWP"
     gene            complement(2615..3184)
                     /locus_tag="VF240_16800"
     CDS             complement(2615..3184)
                     /locus_tag="VF240_16800"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7176922.1"
                     /translation="MSDTTKIIAFALCAGLAILAPGGASPAHAQRKAGGGGTVACDAE
                     AYANDPDPRGMNVRSGPGANYQVVGNLPNQDVDGILVHLTGSQGEWVRIDRAVEQGGD
                     TERVLFKGMGWVHGSLLSVDGVGGLDGGSPLYRGPVAKGRVLVRVPVDGGGATVRGCR
                     GRWMYVEHKGVKGWAAPGTLCSNALTNCS"
     gene            complement(3334..3915)
                     /locus_tag="VF240_16805"
     CDS             complement(3334..3915)
                     /locus_tag="VF240_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176923.1"
                     /translation="MRNKTLIGIAFLVGTSFSLLARGQRAELQTLPQTITLFSRITHK
                     DDGYGRSAFNFRHGVRSDVEGWERLTFNRAHLLYGSISIDGDTDWFSVSMGGEDPSRI
                     KDLGELEWSDIHHTPFLPANPQLSTGIRFPGIDETFESSSDGRVTQAVAGHMYVVHIK
                     NRESDLYAMFRVDALERNDHCAISWKVVPSPED"
     gene            4022..4282
                     /locus_tag="VF240_16810"
     CDS             4022..4282
                     /locus_tag="VF240_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEX7176924.1"
                     /translation="MSDLSGNVRHPREMGATEVTAFLSHLATARNVSASTQNQALSAL
                     LFLYRDVLGMTLPWLGDPLTSRLSSPGRRCAPSSRTAPARSG"
     gene            4349..4579
                     /locus_tag="VF240_16815"
     CDS             4349..4579
                     /locus_tag="VF240_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176925.1"
                     /translation="MDFGYGEVVVRDGKGGKDRRTPLPGPLSDPFRIHLWRVKALHEH
                     DLRAGFGCVELPQALSRKYPRACRESRKRCAR"
     gene            complement(4783..>5505)
                     /locus_tag="VF240_16820"
     CDS             complement(4783..>5505)
                     /locus_tag="VF240_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176926.1"
                     /translation="YLDFFNREPDASGLAFWMGQTTGCGNADLQVCRNNVSGAFFVSG
                     EFQETGYFVYKMYQAAYGVRAGEPVPTTLREFLPDLQQVGRGVVIGQAGAEAQLEANK
                     AAYAAEFAARARFAALNAMTPAEFVNALHANTENALTQAERDSLVSRLTLGQITKARA
                     LREVAENGAVNQKFFTRAFVLMQYYGYLRRNPYDAPELNLDFGGYNFWLGKLNQFGGN
                     HISSQMVQAFITSGEYERRFGP"
BASE COUNT          928 a         1936 c         1723 g          918 t
ORIGIN      
        1 ggcttgaagt tctttcgccc ctccggggct tcaaatactt tccggcatct gttccccggt
       61 ctcacgaccg gggaacagat gccgccgcct ccggcggctg gcggtgccta tgacttctaa
      121 cgcgcgccct tcgccgcatt cacctacttc ttcttgaata cgtcgtcctc cgggttaacg
      181 aagacggcct gaaacggccc cgtgccatgc acctgaacga tggtctcgcc cttcgacagg
      241 gcgtagtgcg ggacgttctt cggcgagacg aggtaagcgc ccgcgccgaa ctcctgcgcg
      301 cccgtcaggt cgtacttctc tcccatgccc atgacccacg ttcccgtgat gacggttaag
      361 tgctcgtcga acgggtgcca gtgcggcggc accttcagcc cgtcgcggtg cttgatgcgg
      421 atgacgtagt gcgagccggc ggctgcgggg tcgccttcca gcacggcgat ctccgcgccc
      481 tcgtagaagg gcttccactg aatcgtctcc ggctggagcg cgacgtgaaa gctgtgtccg
      541 gcgacgtgct cggccggcgc gacgtgcatc gcgggcgcga tctcggccgc ccgctcccgg
      601 cccgcctgcg cgaagatggc gcacgccgcc agcagcacga gtgggtatgc gaaaatgtgt
      661 cttctcatcg tctcgcgtct cctcccggtg gccccgttga gccgtcctac tcttaacgcg
      721 cagtgcggcg gcgaaaagga tcatctccca cgctcgtggg cgaacgcgcg cgccggcggg
      781 ggccgtggcg cgcggcgaac agttcacgcc tgttgtggcc gcgtgcgccg gccgttatac
      841 tccaacgtgc gggcctgctc gccacgcgcg gcgcggcgac aaagtctcga aatttatccc
      901 gcgaagcgga cgcgcgggcc cgacacgccg caccggccgc cgactgctct catggctcag
      961 gaactcttca actcacagga ttggataagc gcggccgacc gcggcgacgc ggacaccgtc
     1021 gaaaggtttc tggccgaggg cgccgacgtt gacgtgtgca cgccgggcag cgagacggcc
     1081 ctgatgcgcg ccgccgcgcg tggccacgcc ccgctcgtcc gcgtgctcct cacgcgcggc
     1141 gccgacccga accttagtcg gcgcgacggc tggacggcgc tcatgctcgc agccttcttc
     1201 ggccacgccg agacggcgcg cgaactcctg cgccgcggag ccgacatcga ggcgaccgac
     1261 cgcaacggct cgacggcctc caagtgggcc acctcgcgcg gccacctcga ggtcgcgcgg
     1321 ctcatcaacg acgcctcagc ggcgggcgtg tcgattgaga aggagattga aaaggttgcg
     1381 cccgtcgtgg agacggggaa ggacgcgaag gccgacacgc tgcggccaat cgcgccgacg
     1441 ccggagcgtg cgccggaaat caacgccgcc gttacgccgc cgatactgac gccgacgagc
     1501 gccgcaaggc gggtcgaacc agcgggcgat gcgcgggaag aattggacga agtcaccatc
     1561 gccaccgccg tcaggaagtc ctccgccgcg cctgccgcgc cgacccacgc tcccacaccc
     1621 gcgcaggtct tcatgcaagc gcacgcaccc gcaccaatcc cggcgcacac gcacgcgccg
     1681 ccggccgctt caatcttcga gcgcgcggat gagttgcgtg cggagatgtt agccgtttca
     1741 cctgaggtca ggagggaagt taacgagctg gccgcgcgcg cggcgcggcg cgcggacggc
     1801 ggcgcacgct tctggcgctc ggcggcgctc gggatggcgc tcctggccgt cggcgccgcc
     1861 ctgctcgctt acaaattcgg tggcgcgggc gcaccctcgg ccggcgacgg cgcgcgcttc
     1921 gcggccacgc ccgaggcgtc ggcgcaaccc gcgacggccg cgcccatcga gatgcagccg
     1981 gccgtgcccg tcacgcccat cgcctcgccc ctgcccgcag accccgcctc gcagggcgtc
     2041 ggcacggtgg gccttgcgcc ggggacacag ccgagcgtcc cgacatactt cccgcccgcc
     2101 acgaccgacg cggcgaccac ggtcgagacc gcccggccgc gtgagacata tccggccaac
     2161 cagccggccg ggcgcgcgtc cggcggcgat ccggcattgg ttaatctcgg gcagtcggag
     2221 cgaacggccg acgatgcgcc ggcgcttccg acgcgtcggc cacaggactc cgccgcgtcc
     2281 gacaacacgc gcaccgaggc cccggcgacg cgccccgcgc gcgacgactc cgcgcagaga
     2341 agcacccgca ccacccgtcc ggccgacgag ccgcgcccca caccgacgcc gaagggcaaa
     2401 gtcgtcaact ggccctgacc ccgccacgtc ggacggacgc cacctatttt cagcttaact
     2461 actctagccc ccctcttttt ataagtttca tgggccggcc gcagggatcg ggtcttaatt
     2521 gatttgtgaa ggccctcccc ttggggtgta tgagcagcgg ggcgatgcgc ggtcgggtgc
     2581 atgccgttgt caggtggcgc acgcggcccc cggtttagct acaattcgtg agtgcattcg
     2641 agcagagcgt gccaggagcc gcccaaccct tcaccccctt atgctcgacg tacatccacc
     2701 ggccacggca tccacgcacc gtggccccgc ccccgtcaac cggaaccctg acgagaacgc
     2761 ggcccttcgc cacgggcccg cgatagagag gactcccacc gtccaacccg cccacgccgt
     2821 cgacgctgag cagcgagccg tgtacccacc ccatcccctt gaagagaacc cgctccgtgt
     2881 caccgccctg ctctaccgcg cggtcgattc tcacccactc accctgcgag cccgtgagat
     2941 ggacaaggat gccatcgaca tcctgattcg gcagattgcc gacgacctga taattagctc
     3001 ccggccccga gcgcacgttc atcccgcggg ggtccgggtc gttggcatac gcctccgcgt
     3061 cgcaggcaac ggtgccgccg ccgcccgcct tccgctgagc gtgcgcgggg ctggcgcccc
     3121 ctggggcgag gatggccagc cccgcgcaga gggcgaacgc aataattttg gttgtgtcac
     3181 tcatagcgca ccttctctca gtgccagaat aacattgggg catctaacgc ccggcatcag
     3241 ccgcgcgcac gggccataga tgataaaagc caacgtgatt tgcgcgtccg ctgcataccg
     3301 ttgttaggcg cggctgataa tttactcctc ggtttaatcc tcaggcgacg gcactacctt
     3361 ccaggagata gcgcagtgat cattacgctc aagagcatcg acccggaaca tcgcatacag
     3421 gtcagattcc cgattcttga tatgcaccac gtacatatgg ccggccactg cttgtgtcac
     3481 ccggccgtcg gaagatgact cgaacgtttc atctatccct gggaatctaa taccagtcga
     3541 cagctgaggg ttagccggga ggaagggcgt gtggtggatg tcagaccact ctaattcgcc
     3601 caaatcttta atccggctcg ggtcttcacc acccattgag acgctgaacc aatccgtatc
     3661 gccatcaatg gagatactcc catacagaag atgtgcacga ttgaaagtga ggcgctccca
     3721 tccttccaca tcgctcctga cgccatgccg gaaattgaag gctgatcttc cgtaaccatc
     3781 atccttgtgt gtgattctgg agaagagcgt gattgtttgg ggtagagttt gcaactcagc
     3841 cctctggccg cgcgctagca aactgaacga cgtgccaacc agaaaggcaa tacctatcaa
     3901 ggttttgttt ctcacgcccg cctcctttca actttgcgcc taacgccgga gctaagccgc
     3961 cgcgcatcca actgcgacag cgccaagttc tcgataagag gccgcctgat gcgcggtcgg
     4021 cttgagcgac ttgtcaggca acgtgcgcca ccccagggag atgggcgcca ccgaggtgac
     4081 ggcgttcctc tctcacctcg ccacggcccg caacgtctcg gcctcgaccc agaaccaggc
     4141 gctctcggcc ctgctcttcc tttaccggga tgtgctcggc atgacgctgc cctggctcgg
     4201 cgacccgctc acgtcccggc tgtcttcacc agggaggagg tgcgcgccgt cctctcgcac
     4261 cgctccggcc agaagtggct gatggcttcc ttcctctacg gctcggggct caggctctcc
     4321 gagtgtctga agttgagggt cgaggtgcgt ggacttcggc tatggtgagg tcgtggtcag
     4381 ggacggcaaa ggcggcaaag acaggcgcac gccgctaccc ggcccactgt ccgatccctt
     4441 ccgcatccac ctctggcgtg tgaaggccct gcacgagcac gatctgcgag ccggcttcgg
     4501 ctgcgtcgag ttgcctcagg cgctctcgcg taagtacccg cgcgcctgcc gcgagtcaag
     4561 gaagcgatgc gcgcggtaag gctcacacca agcacggggg ctgtcacacc cttcgccact
     4621 cgttcgccac gcacctgctc gatgacggct acgacctgcg gacgatccag cagctgtagc
     4681 ggcggggcgt ggagccacac cggtaaaggc acgtcgcgga cgcccttcct gatttttggc
     4741 ggcaggaagg cccaccgccg agggggcaga gttggtggcc tatcacggcc cgaagcggcg
     4801 ctcgtactcg cccgaggtga tgaaggcctg caccatctgc gagctgatgt gattgccgcc
     4861 gaactggttg agcttcccca gccagaagtt gtacccgccg aagtccaggt tcaattccgg
     4921 cgcatcgtag gggttcctgc gcagataccc gtagtactgc atgaggacga aggcgcgggt
     4981 gaagaacttc tggttgacgg ccccgttctc ggccacctcg cggagcgccc gcgccttcgt
     5041 gatctgcccg agcgtcaggc ggctgacgag gctgtcgcgc tcggcctgcg tgagcgcgtt
     5101 ctccgtgttc gcgtgcagtg cgttgacgaa ctccgcgggc gtcatcgcat tgagcgcggc
     5161 gaagcgcgcg cgcgcggcga actcggcggc gtaggccgcc ttgttggcct ccaactgcgc
     5221 ctcggccccc gcctgcccga tgaccacccc gcggccgacc tgctggaggt cgggcaaaaa
     5281 ctcccgcagc gtcgtcggca ccggctcgcc cgcgcgcacc ccgtaggccg cctgatacat
     5341 cttgtagacg aagtagcccg tctcctgaaa ctcgccggag acgaagaagg ccccggagac
     5401 gttgttgcgg cagacctgca agtcggcgtt gccgcagccc gtcgtctggc ccatccagaa
     5461 ggcgaggccc gaggcgtccg gctcgcggtt gaagaagtcg aggta
//
LOCUS       DASVES010000410         6442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1238914, whole genome shotgun sequence.
ACCESSION   DASVES010000410 DASVES010000000
VERSION     DASVES010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238914"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VF240_16825"
     CDS             complement(<1..296)
                     /locus_tag="VF240_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176927.1"
                     /translation="MSTNNQNGHRGADENPRQQKRRTPWPLMVVAALLVIVPFAYWYG
                     TWFGRSLSDEQIEKYFNEGGNPRHVQHALSQIGEGLEAGKPGVGRWFPRVVEASG"
     gene            complement(334..756)
                     /locus_tag="VF240_16830"
     CDS             complement(334..756)
                     /locus_tag="VF240_16830"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX7176928.1"
                     /translation="MDHTALPHLNAALNATSFLLLLAGFYFISRRQIGAHRACMLGAL
                     GVSMLFLVSYLIYHFGYRGMTRFTGQGAVRYFYLTILLTHTVLAVVIVPLVALTLGRA
                     LRGDFPRHRRIARWTFPIWLYVSATGVVVYVMLYHLYR"
     gene            complement(780..1028)
                     /locus_tag="VF240_16835"
     CDS             complement(780..1028)
                     /locus_tag="VF240_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176929.1"
                     /translation="MSEKEDLTLRVLRLFPEWGRETLDLHELFEAGDSNDPDARREVL
                     YTVEMLVGEGLLEERGNDFYALSPEGRRVLQEQAGGEG"
     gene            complement(1064..1465)
                     /locus_tag="VF240_16840"
     CDS             complement(1064..1465)
                     /locus_tag="VF240_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7176930.1"
                     /translation="MSEADNVSVVQRAFEAFGGGDVPAFLSFLSEDVEWTIAGPPSVP
                     YAGERRGHEGVTEFLQAIGGAVEFERFEPQEFIAQGDRVAVVGFERARLRSSGRTFDN
                     PWVLLFTLKDGKITRFRSYEDTYAVAQSFAG"
     gene            complement(1468..1698)
                     /locus_tag="VF240_16845"
     CDS             complement(1468..1698)
                     /locus_tag="VF240_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176931.1"
                     /translation="MTQDDRLARIDVSRQTTRGRACPSCGGKIIPSDRAIYQAAAGTT
                     DTFPLWQCERCGYEEMGQKPKAPPKPSHPKKV"
     gene            complement(1685..1975)
                     /locus_tag="VF240_16850"
     CDS             complement(1685..1975)
                     /locus_tag="VF240_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176932.1"
                     /translation="MRHSTERAWRSTPLRLAALIAVGLVLVLIAPAGADAQCAMCKSA
                     VQGAGEQATRAMNSGILVLLIPPVAIFCSIFAVFIKYKNGDERADTEKHDAR"
     gene            complement(1972..2589)
                     /locus_tag="VF240_16855"
     CDS             complement(1972..2589)
                     /locus_tag="VF240_16855"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX7176933.1"
                     /translation="MSRVLSSARGLFGKQDVLLVFLCVLLAGVFLALAATNLSGGISG
                     LLSIDGLFLTAVALTMALVFISIPAMYLRERGMLNNPFTLGEGVPAARTAEAADLHFE
                     GTTKLFLSVLGALLFLTLIEVLLAYFKVSLLVMLTLLIGLSLIKAALIIAYFMHLRFE
                     RLSLVLTLIPILCICICLLFVFFPDSRRSHDLRAVQVKDAVEEAR"
     gene            complement(2612..3283)
                     /locus_tag="VF240_16860"
     CDS             complement(2612..3283)
                     /locus_tag="VF240_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7176934.1"
                     /translation="MSSEAHAHAGQVITADWGGGTLPYRVGHKKLGMWLFIVSDAMTF
                     SAMLIVYSYVRASSDEWPTPYEFYPSIIFSTVMTLCLLTSSYTMVKAVQASAHNNLRS
                     ARRWLFGTILGGGAFLVLHIIEWINVIDHGLRPFSLPAEWAQRWPNAPPLFGASFFAI
                     TGLHMFHVLTGAIYLGWIMLRIKHTRHEDVEISGLYWHFVDLVWMFVFPLIYLMSFDP
                     TAGGH"
     gene            complement(3290..4006)
                     /locus_tag="VF240_16865"
     CDS             complement(3290..4006)
                     /locus_tag="VF240_16865"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7176935.1"
                     /translation="MVTTVTGTEQQRAVRTKKGIGLGPHPPGGNGSRKNGGGGDRSPG
                     GGGEEGERWSPDRYRIGVYVGLASIVMMFTALASAYIVRAGLPTSTDWRPTAMPSVVV
                     VSTVLIVISSLTMMLAQKSLGRGDEQPFRRWLALTLLLGLAFLGSQLLAWRQLVAQGV
                     YLATNPHSSFFYVLTGLHGLHLLGGILGLSYLLLRAARGAGGGEAGAKRRAVSHMVGV
                     YWHFMDGLWVFLFMLLFFWR"
     gene            complement(3981..5885)
                     /locus_tag="VF240_16870"
     CDS             complement(3981..5885)
                     /locus_tag="VF240_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7176936.1"
                     /translation="MAEVDPTHHETHEEADVHGASAAAHAAHHHGPPTGFIRKYIFSI
                     DHKVIGIQYIFLALFSVFVGMFMSVLMRIKLTWPMTGVPVLETIFGAAAAPGGVMSPE
                     LYLSLVTMHGTMMVFFVLTTAPQGGFGNYILPIQVGADDMAFPVLNMLSFWVTLVGLI
                     VLVAAIFASGSGVVGPTGGWTGYPPLSAVGHLLNVPGMGMGMNLWIISIAIFCVGSLL
                     GALNFITTLLNLRTRGMSLMRMPLTCWSWFTTAVLALLAFPVLLAAGILLFLDRNAGT
                     SFFIPTGLSDGTRVLDHQGGSPLLWQHLFWFFGHPEVYIAILPGMGVTSHILSTFARK
                     PVFGYRAMVFAMFAIGLLGFFVWGHHMFMSGMSPYSAMAFSVLTLAIGVPSAIKTFNW
                     LGTLWGGKIRFTTPMLFALGFVSLFVAGGITGLVLGQTTLDFSMHDTYFVLAHFHLVM
                     GVAAIFGMFAGIYFWFPKMFGRMMSEKLGKIHFWPTMIGVFTIFVPMHAMGNVGMPRR
                     FSSFMEYRFLDRLDGLVMLVTIAVIITAASQFIFLFNFFWSMFKGTRASGNPWEATTL
                     EWATPTTPPPHDNFGGNVPTVYRGAYEFSVPGAERDFIMQHETEAEGRGDESLRGQEG
                     KGNGHNGHGH"
     gene            complement(5938..>6442)
                     /locus_tag="VF240_16875"
     CDS             complement(5938..>6442)
                     /locus_tag="VF240_16875"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176937.1"
                     /translation="AGSAQIRVLAQQFAWNFHYAGPDSRFGRTDPNLIDDSSSPPRGN
                     IGLDETDAAAKDDAVSSTLITQVDRPVELVLRGRDVTHSIWVPELRFKQDLVPGMDIR
                     VHFKPQKVGRYELACAELCGALHYQMKSYLLVLPEQQYAELMQLPQSEFTRRVTQLYE
                     QYPLPQY"
BASE COUNT         1282 a         2002 c         2116 g         1042 t
ORIGIN      
        1 cccgaggctt cgacaacgcg cgggaaccag cgcccgacgc ccggcttccc cgcctccaac
       61 ccttcaccta tctgcgacag tgcgtgctgg acgtggcgcg ggtttccacc ctcgttgaaa
      121 tacttctcga tctgctcgtc gctcaagctc cggccgaacc atgtgccgta ccagtaggcg
      181 aacggcacga tgactaacag cgcggccaca accatcagcg gccatggcgt gcgccgcttc
      241 tgttgccggg ggttttcgtc cgctccccga tgaccgttct ggttgttcgt tgacacttcc
      301 tttacgccgc gcccgccgcg tctcgtcccc gcattagcga tacaaatgat aaagcatgac
      361 gtaaaccacg acgcccgtcg cggagacgta gagccagatg gggaaagtcc aacgcgcgat
      421 gcggcgatgt cgggggaagt cgccgcgcag tgcccggccc agagtcaggg caactaaggg
      481 gacgatgacg acggcgagaa cggtgtgagt cagcaggatc gtcaggtaga aatagcgaac
      541 tgctccctgc cccgtgaacc gcgtcatccc tcggtaaccg aaatgataaa tcaggtacga
      601 gacgagaaat aacatggaga cgccgagcgc cccgagcatg caggcgcggt gcgcgccgat
      661 ctggcggcgg gagataaagt aaaagccggc gaggagcagg agaaagctcg tggcgttgag
      721 ggcggcgttg aggtgcggga gggctgtatg gtccatcaac aataattcta acagaaggtc
      781 taaccttccc cgcccgcttg ctcctgcaaa accctgcggc cctccggcga gagcgcgtaa
      841 aaatcgttcc cgcgctcttc gagcagacct tcgccgacca gcatctcgac cgtgtagagg
      901 acttcgcggc gcgcgtcggg gtcgttgctg tcgcccgcct cgaacagttc gtggaggtcg
      961 agcgtttccc tcccccactc ggggaacagg cgcagtacgc ggagagtcaa atcttccttc
     1021 tcagacatct tcaacatgtc gggcggccgc gcgacgcttc gtctcaaccg gcgaaggatt
     1081 gcgccacggc gtaggtgtct tcataagagc ggaagcgcgt gatcttcccg tccttgagcg
     1141 tgaagagcag cacccacgga ttatcgaacg tccgcccaga ggagcgcagc cgggcgcgct
     1201 cgaagccgac gacggctact cggtcgccct gcgcgatgaa ctcctgcggc tcgaaccgct
     1261 cgaactcgac cgcgccgccg atggcctgca agaactccgt cacgccctcg tgcccgcgcc
     1321 gctcgcccgc gtaaggcact gacggcggcc cggcaatcgt ccactctacg tcttcggaga
     1381 ggaagctgag gaaggcaggc acgtcgcccc cgccgaaagc ttcgaaggcc cgctgaacca
     1441 cgctcacgtt gtcggcttcg ctcataatta gactttcttg ggatgggagg gtttgggcgg
     1501 cgccttcggc ttctggccca tctcctcgta gccgcagcgt tcgcactgcc acagcgggaa
     1561 ggtgtctgtc gtgccggcgg ccgcctgata gatggcgcgg tcggaaggga tgatcttgcc
     1621 gccgcaggag gggcacgcgc gccccctcgt ggtctgccgg ctcacgtcga tccgcgcgag
     1681 tctgtcatct tgcgtcatgc ttctccgtgt cggctcgctc gtcgccgttt ttgtacttga
     1741 tgaagacggc gaagatcgaa cagaagatgg cgaccggcgg gatgagcagg acgagtatcc
     1801 ccgagttcat cgcccgcgtg gcctgttcac cggcgccctg aacggccgac ttgcacatcg
     1861 cgcactgcgc gtccgccccg gccggcgcga tcaggacgag caccaacccc acggcgatta
     1921 gtgccgcgag acgtaggggg gtacttcgcc atgcgcgctc agttgaatgt ctcaccgggc
     1981 ctcctccacc gcgtctttga cctgtaccgc gcgcaggtcg tgggagcgcc tgctgtcggg
     2041 gaagaagacg aagagcaggc agatgcagat acagaggatg gggatgagcg tcagcacgag
     2101 gctaagccgc tcgaagcgga ggtgcatgaa gtaagcgatg atgagcgcgg ccttgatgag
     2161 cgagaggccg atcagcagcg tcagcatgac aagtaggctg actttgaaat aggccagcag
     2221 cacctcgatg agcgtcagga aaagtagtgc gccgagcacc gagaggaaga gtttggttgt
     2281 cccctcaaag tgcaggtcgg cggcctcggc ggtgcgcgcc gcggggacgc cctccccgag
     2341 cgtgaacgga ttgttgagca tcccgcgctc gcgcagatac atggcgggga tgctgatgaa
     2401 gacgagcgcc atcgtgagcg cgacggccgt caggaatagg ccgtcgatac tcaggaggcc
     2461 gctgatgccg cccgagaggt ttgtcgccgc aagcgccaga aagaccccgg caagcagcac
     2521 gcacaaaaac accaaaagta cgtcctgctt gccgaacagt ccccgcgcgg aactcaaaac
     2581 acgactcatc cgtttagctc ctcctctctc ttcagtgccc cccggcggtc gggtcgaacg
     2641 acatcaggta gatgagcggg aagacgaaca tccagaccag atcgacgaag tgccagtaga
     2701 ggccgctaat ctccacgtct tcgtggcggg tgtgtttgat ccgcagcatg atccacccga
     2761 ggtagatggc gcccgtcagg acgtggaaca tgtgaaggcc cgtgatggcg aaaaaggacg
     2821 cgccgaacag cgggggcgcg ttcggccacc tttgcgccca ctcagcgggg agcgagaacg
     2881 gcctcagccc gtggtcgatg acgttaatcc actcgatgat gtggagcacg aggaacgcgc
     2941 cgccgccgag gatcgtcccg aaaagccaac gccgggcgga gcgcaggtta ttgtgcgccg
     3001 acgcctgcac ggccttgacc atcgtgtaac tcgacgtgag caggcacagc gtcatcacgg
     3061 tggagaagat gatgctcgga tagaactcgt agggcgtcgg ccactcgtcg ctcgacgcgc
     3121 ggacgtagct gtagacgatg agcatcgccg agaaggtcat cgcgtccgac acgatgaaga
     3181 gccacatccc gagtttcttg tggccgacgc ggtagggaag tgtgccgccg ccccagtccg
     3241 ccgtaatcac ctgccccgcg tgcgcgtgcg cttcgctgct caagacgcgt cacctccaga
     3301 agaataggag catgaacaaa aacacccaca ggccgtccat gaaatgccaa tagacgccca
     3361 ccatgtgcga gacggcccgc cgcttcgccc cggcctctcc gcccccggcc cccctcgcgg
     3421 ccctcaacag aaggtaactc aggccgagga tgccgcccaa taggtgcagg ccgtgcagcc
     3481 ccgtcagcac gtagaagaaa gagctgtgcg ggttcgtggc gagatagacg ccctgcgcca
     3541 cgagttgccg ccacgcgaga agctgcgacc ccagaaaggc gagacccagc aagagtgtca
     3601 gggccagcca acgcctgaag ggctgttcgt caccgcggcc caggctcttc tgcgcgagca
     3661 tcatcgtcag actgctgatg acgatgagca cggtgctgac gacgacgacc gagggcatcg
     3721 ccgtggggcg ccagtcggtc gaagtcggca accccgcgcg gacgatgtag gcgctggcga
     3781 gcgccgtgaa catcatcacg attgaggcga ggccgacgta gacgccgatg cggtagcggt
     3841 cgggtgacca acgctcgccc tcttccccgc cgccgccggg tgagcggtcg ccgccgccgc
     3901 cgttcttgcg cgagccgttg ccgcccggtg ggtgcggccc gaggccgatt cccttcttcg
     3961 tgcggacggc gcgctgctgc tcagtgcccg tgaccgttgt gaccattccc cttgccctct
     4021 tgccccctca gactttcgtc accccgcccc tcggcttccg tctcgtgctg catgatgaaa
     4081 tcgcgctcgg cgccggggac ggagaactcg tatgcgccgc gatagacggt cggcacgttg
     4141 ccgccgaagt tgtcgtgcgg cggcggggtc gtcggcgtcg cccactccag cgtcgtcgcc
     4201 tcccacggat tgccggaggc ccgcgtcccc ttgaacatgc tccagaagaa gttgaagagg
     4261 aagatgaatt gcgacgcggc cgtgatgatg acggcaatcg tgaccagcat caccagcccg
     4321 tcgaggcgat caaggaacct gtactccatg aaggacgaga agcggcgcgg catgccgacg
     4381 ttgcccatcg cgtgcatcgg gacgaagatg gtgaagacgc cgatcatcgt cggccagaag
     4441 tgaatctttc ccagcttctc actcatcatc cgcccgaaca tcttggggaa ccagaagtag
     4501 atgccggcga acattccgaa gatggcggcc acgcccatca cgaggtggaa gtgcgcgagc
     4561 acgaagtagg tgtcgtgcat cgagaagtcg agcgtggtct ggccgaggac gagacccgtg
     4621 atgccgcccg cgacgaagag cgagacgaag ccgagcgcaa aaagcatcgg ggtggtaaat
     4681 cgtatcttgc cgccccagag cgtgccgagc cagttgaagg tcttgatggc cgagggcacg
     4741 ccgatggcca gcgtcaatac cgaaaaggcc atcgccgagt agggactcat gccgctcatg
     4801 aacatgtggt ggccccagac gaagaagccc aaaagcccga tggcgaacat ggcgaagacc
     4861 atcgcgcggt agccgaagac gggcttgcgt gcgaaggttg agaggatgtg cgaggtgacg
     4921 cccatgcccg gcaggatggc gatgtagact tcggggtggc cgaagaacca gaagaggtgc
     4981 tgccacaaaa gcggcgagcc gccctgatgg tcgagcacgc gcgtgccgtc gctcagaccc
     5041 gtcgggatga agaagctggt gccggcgttg cgatcaagga acaggaggat gccggcggcg
     5101 aggagcaccg gaaacgcgag gagcgccagc acggccgtcg tgaaccacga ccagcacgtg
     5161 agcggcatcc gcatcaaaga catcccgcgc gtgcgcaggt tcaggagcgt ggtgatgaag
     5221 ttgagcgcgc ccaagagcga gccgacgcag aagatggcga tactgatgat ccagaggttc
     5281 atccccatgc ccatgccggg gacgtttaag agatgcccga cggcgctcaa gggcgggtag
     5341 cccgtccagc cgccggtcgg cccgacgacg ccgctgcccg aggcgaagat ggcggcgacc
     5401 aggacgatga ggccgacgag cgtcacccag aaggagagca tgttgaggac ggggaaagcc
     5461 atgtcatctg ccccgacctg aatgggaagg atgtaattgc cgaagccgcc ctgcggcgcg
     5521 gtcgtcagca cgaagaagac catcatcgtg ccgtgcatgg tcacgagcga cagatatagc
     5581 tccggcgaca tcacgccgcc cggcgcggcc gccgccccga agatcgtctc cagcacgggc
     5641 acgcccgtca tcggccacgt cagcttgatt cgcatcagca ccgacatgaa catcccgacg
     5701 aagaccgaga acagggcgag gaagatgtac tggatgccga tgaccttgtg gtcgatgctg
     5761 aagatgtact tcctgatgaa ccccgtgggc gggccgtggt ggtgcgcggc atgagcggcg
     5821 gcggaggcgc cgtgaacgtc ggcctcctcg tgcgtctcgt ggtgagtggg atcgacctcg
     5881 gccatctctc ttgtgtcctc ccggcgcgag cgagcgccaa ggctatctca gggttcatca
     5941 gtactgcggt aacgggtact gctcgtagag ctgcgtgacg cggcgggtga actcactctg
     6001 cgggagctgc atcagctcgg cgtactgctg ctcaggcaga acgagcaggt agctcttcat
     6061 ctgatagtgg agtgcgccgc acaactcggc gcaggccagc tcgtagcgac ccaccttctg
     6121 cggcttgaag tggacgcgaa tgtccatgcc ggggacgagg tcctgcttga agcgcaactc
     6181 gggcacccag atgctgtgtg tcacgtcgcg gcctcgcaag acgagttcga cgggccgatc
     6241 cacctgcgtg atgagtgtgg agctgacggc gtcgtccttc gcggcggcgt ccgtctcgtc
     6301 gaggccgatg ttgccgcgcg gtggcgagga ggagtcgtcg atgaggttgg ggtcggtgcg
     6361 cccaaagcgg ctgtccgggc cggcgtagtg gaagttccac gcgaactgtt gcgcgagcac
     6421 cctgatctgc gccgagccgg cc
//
LOCUS       DASVES010000411         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_497277, whole genome shotgun sequence.
ACCESSION   DASVES010000411 DASVES010000000
VERSION     DASVES010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497277"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VF240_16880"
     CDS             complement(<1..1424)
                     /locus_tag="VF240_16880"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7176938.1"
                     /translation="MTRILGISAFYHDSAACLVVDGRVVAAAQEERFSRVKHDHRFPA
                     HAARYCLEEAGLSASELDYVGFYDKPLLKFDRLLETYLDYAPAGFPSFLKAMPLWMRE
                     KLWMPDLIRTELAKAEGAEDHREAKRRAKRYEFKLLFGDHHESHAASAFYPSPFEEAA
                     VLTIDGVGEWATSSIGVGRGHELKLLKELRFPDSLGLLYSAFTYYTGFRVNSGEYKLM
                     GLAPYGEPRFVPIIKDKLLEVRDDGSLRMNHEFFSYSQGLRMTNRRFDKVFGGRPRRP
                     ESRITQREMDLASSIQCITEEVMSKMVAHTHRETGMRRLCMAGGVALNCVSNGRILRE
                     GPFEDIWIQPAAGDAGGAVGIALSIWHRYLDKPRTSPEKAGTWRSPRDAHNGDIPPYA
                     DGMSGSYLGPRDTEENIEEFLKSHSLPYKKYEREELPGAVADLLAAEKVVGLHQGRME
                     FGPRALGARSIIGDARSPRMQSVM"
     gene            complement(1516..1674)
                     /locus_tag="VF240_16885"
     CDS             complement(1516..1674)
                     /locus_tag="VF240_16885"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEX7176939.1"
                     /translation="MSKMQVFSELWEFMKHNKKFWLAPIIITLLLVGFLLAVTQGSAL
                     APFIYTLF"
     gene            complement(1694..2092)
                     /locus_tag="VF240_16890"
     CDS             complement(1694..2092)
                     /locus_tag="VF240_16890"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HEX7176940.1"
                     /translation="MKKRSYRAERDFGLVVGTVFTLLGAWWLYRGKFEWVAPVMLCVG
                     AALVLSGLVLPRALVLPNRGWMALASVLGYVMTRVVLALVYFLLVTPIGVVKRLTGWD
                     PLGRRTRRGESYWQTYPERQQDPRHFEKMY"
     gene            complement(2300..>2620)
                     /locus_tag="VF240_16895"
     CDS             complement(2300..>2620)
                     /locus_tag="VF240_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX7176941.1"
                     /translation="FLYVEDCADGILRAAALYDDSPPVNIGNGREVFISELVETVARM
                     TGFEGEIRWQPARPNGQPKRQLDTTRAAEKFGFRAQVTLEEGLRRTIAWYENTFPAAN
                     AQKN"
BASE COUNT          472 a          844 c          864 g          440 t
ORIGIN      
        1 ttcatcaccg actgcatgcg gggcgagcgc gcgtcgccga tgatgctgcg tgcgcccaaa
       61 gcgcgcgggc caaactccat gcggccctga tgcagcccga cgaccttctc cgcggcgagc
      121 aggtcggcca ccgcgccggg caactcctcg cgttcgtact ttttgtaagg cagcgagtgt
      181 gacttcagaa actcttcgat gttctcttcg gtgtcgcgcg ggccgagata agagccgctc
      241 atgccgtcgg cgtagggcgg gatgtcgccg ttgtgcgcgt cgcgcggcga gcgccacgtc
      301 ccggccttct cgggactcgt gcgcggctta tcgaggtagc ggtgccagat ggagagcgcg
      361 atgccgacgg cgccgccggc gtcgcccgcc gcgggctgaa tccagatgtc ctcgaagggg
      421 ccttcgcgca ggatgcgacc gttcgagacg cagttgagcg ccacgccgcc ggccatgcag
      481 aggcggcgca tcccggtctc gcggtgcgtg tgggcgacca tcttcgacat cacctcctcg
      541 gtgatgcatt ggatgctgct cgccaaatcc atctcgcgct gtgtgatgcg cgactcgggc
      601 cggcggggac ggccgccgaa gactttgtcg aagcgccggt tcgtcatccg caagccctgc
      661 gagtagctga agaactcgtg gttcatccga aggcttccat cgtcgcgcac ttcaaggagc
      721 ttgtccttga tgatggggac gaagcgcggc tcgccgtagg gggcgaggcc catcaacttg
      781 tactcgcccg agttgacgcg aaagcccgtg taataagtga aggccgagta gagcaggccg
      841 aggctgtcgg gaaagcgcag ctccttcaaa agtttcagct catggccgcg gccgacgcca
      901 atggaagaag tcgcccactc gccgacgccg tcgatggtca gcaccgccgc ctcctcgaac
      961 ggcgacgggt agaaggccga ggcggcgtgg ctctcgtggt ggtcgccgaa gaggagtttg
     1021 aactcgtaac gcttggcgcg ccgcttcgcc tcccggtgat cctccgcgcc ctcggccttc
     1081 gccagctccg tgcggatgag gtcgggcatc cacagcttct cgcgcatcca gagcggcatc
     1141 gccttgagga aggaggggaa gcccgccggg gcgtagtcga gataggtctc cagcagccgg
     1201 tcgaacttca aaagcggctt gtcgtagaag ccgacgtaat ccagctccga ggccgacagg
     1261 cccgcctcct ccagacagta gcgcgcggcg tgagccggga agcggtggtc gtgcttgacg
     1321 cgcgagaagc gctcctcctg cgcggcggcg accacgcgcc cgtccacgac gaggcacgcc
     1381 gccgagtcgt ggtagaaggc cgagatgccg aggatgcgtg tcataagaag gtgataagtg
     1441 atgagtgata agtgataaga aagagaaagc gcgccgccgt cttccttctt atcacttatc
     1501 actcatcact tatcactaga acaacgtgta gatgaacggg gcgagcgccg acccctgggt
     1561 gacggcgagg aggaagccga cgaggaggag cgtgatgatg atgggggcga gccagaactt
     1621 tttgttgtgc ttcatgaact cccacagttc actgaagacc tgcatcttcg acatgacgcg
     1681 tgtctcaacc ccttcagtac atcttctcga aatggcgcgg gtcctgctgc cgctccgggt
     1741 aggtctgcca gtagctctcg ccgcgacgcg tgcgccgccc caaagggtcc caacccgtca
     1801 ggcgtttgac gacgccgatg ggcgtgacga gaaggaagta gaccagcgcg aggacgaccc
     1861 tcgtcatcac gtagccgagc acggaggcca gcgccatcca gccacggttc gggaggacga
     1921 gcgcgcgcgg caacaccagc cccgacaaca ccagcgccgc cccgacgcac agcatgacgg
     1981 gcgcgaccca ttcaaattta ccccggtaga gccaccacgc gccgaggagc gtgaagaccg
     2041 tgccgacgac gagtccgaag tctcgctcgg cgcgatacga acgctttttc acggccccgt
     2101 ccaacagtcg cacaccccct ctttctccac ccccggcgga agcggaaaat gatacccgtt
     2161 tcaaccgcca accgccaaca gtaccgcgag cggtagcgag cgggtctgct cgggtaagcg
     2221 acccgctcgc taccgctcgc gttactgtgc gtggcggcgc cggagcgagc gcgtccccgt
     2281 gcgtgcgcgt cgcgggctat cagttcttct gggcgttggc cgccgggaag gtgttttcgt
     2341 accacgcaat cgtccggcgc aggccctctt cgagggtgac ctgcgcgcgg aagccgaact
     2401 tctcggcggc gcgcgtggtg tcgagctggc gcttgggctg gccgttgggc cgggcgggct
     2461 gccagcgaat ctcgccctcg aaacccgtca tgcgcgccac cgtctcgacc agctcgctaa
     2521 tgaacacctc gcgcccgttg ccgatgttga cgggcggaga gtcgtcatag agagccgccg
     2581 cgcgcaggat gccgtcggcg cagtcctcga cgtagaggaa
//
LOCUS       DASVES010000412        20193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1664779, whole genome shotgun sequence.
ACCESSION   DASVES010000412 DASVES010000000
VERSION     DASVES010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20193
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664779"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VF240_16900"
     CDS             <1..495
                     /locus_tag="VF240_16900"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX7176942.1"
                     /translation="VLRMLNEIGSVERVPEYMKRVKAGEFRLMGFGHRVYKNYDPRAR
                     IIKQIAYEVFEVTGKDPLLDLALELERIALEDEYFVKRRLYPNVDFYSGIIYRAMRFP
                     VDMFPVLFAIPRTSGWLAQWAEMLGDPDQKIARPRQIYLGQRTRDYVPMEKRNGQAEG
                     AAAS"
     gene            703..1755
                     /locus_tag="VF240_16905"
     CDS             703..1755
                     /locus_tag="VF240_16905"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX7176943.1"
                     /translation="MLPTRGYATDGPTSQLQPFDFERREPGPRDVLIEIQYCGVCHSD
                     IHQARNEWGNSIYPMVPGHEIVGRVARVGAQVSRFKEGDLAGVGCFVDSCRVCANCKE
                     GEDHYCENHLVATYNGTEMDEKTPTYGGYSSQVVVDEDYTLKVPASLPLPNVAPLLCA
                     GITTYSPLRRFKVGPGQRVGVVGLGGLGHMGVKLAASMGAGVTVFSTSPSKESDARSL
                     GAHDFVVTKDPENLVALAGKFDFILDTVSAAHDLNMYLALLRREAVMVLVGGPEKPLE
                     VQAFSLITNGRTLAGSMIGGIRETQEMLDYCAEHNITSDIELIPIGRIDEAYERTVKG
                     DVRYRFVIDMESLKGK"
     gene            complement(1902..2174)
                     /locus_tag="VF240_16910"
     CDS             complement(1902..2174)
                     /locus_tag="VF240_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX7176944.1"
                     /translation="MDWRVLLATFGTIFLAEMGDKTQLAAMTMAAQTKRPWTVFVGAS
                     LALICVSAVGVLVGGTLGHYLPLEWIKRVAAVAFIVIGVLMLLGKF"
     gene            2232..3938
                     /locus_tag="VF240_16915"
     CDS             2232..3938
                     /locus_tag="VF240_16915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176945.1"
                     /translation="MLGYCAMLQRAEPSQRVSGAHARLWWLIAGRIVAALLLLGASVV
                     WTRFFLQDGIAVAGARAPVPRGALPIGAAVLVLSLVYAAALRLTRLSVVVQAGAQFFC
                     DALLVTWLVSQTGDLHSPYAALYILVIAIASFYLGAREVMLVAAGCALFYTAAMLSIS
                     LGFVSARGAETAAMPLAEVLGTMGLNAAVCLVVGLLAARLADRQTRSDVQLRQTAHAL
                     ASLRRLHERIVDSIRSGVITTDLSRRIYTVNTAAEEMIGYGAADLRGQDVSILFGDIR
                     AQIEQSLRAAAGGHTSPRFEADCLTAEGFRVRLGYSVSPLSGEAGDTTGLVITFQDLT
                     DVRAMEETSRRQERLSAVGRVAAGIAHEIRNPLAAMRGSIQVLRDDLADDPGRQELME
                     IILRESDRLNRIITDFLTYARPRQVSLAAVDLREPLRETFALLRHSPEMRPDHTLEEV
                     LPDEPVAASADENAIKQVFWNLSRNALIAMPEGGSLRAEMETLPQGRVRITFTDSGRG
                     MSRSQVERLFEPFSSSTTGGTGLGLSIVYQIIRDHGGTINVRSREGHGTTITVELPGA
                     KP"
     gene            3982..4290
                     /locus_tag="VF240_16920"
     CDS             3982..4290
                     /locus_tag="VF240_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176946.1"
                     /translation="MTGEEMQKAMDFVVSQQAQFASDIGELKDVVARLANATLSRFEA
                     TDRKRDDVDRRISALVDSQLRTEESIRKTEENISRTEDNVRALTAVVDRYFSEGRNGK
                     "
     gene            4323..5747
                     /locus_tag="VF240_16925"
     CDS             4323..5747
                     /locus_tag="VF240_16925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7176947.1"
                     /translation="MAKLLIVDDEQGMRQLLSLVFGRAGHSVRAAESGRRAVEMLRSE
                     PADVIVSDVKMPDMSGIEMLRAVREFLPDAAVVMMTAFATVETAREAFKLGADDFIQK
                     PFDVDELKLIVEKALERLRLLQENRAFKRAQRERGRLDQIIGHSPRMQAVYQMIETVA
                     AVQSTILITGESGTGKEMVARAIHNLSPRSDKPFMPINCGAFTETLLESELFGYTRGA
                     FTGANANRKGLFEAADKGTIFLDEVGEMSPTMQVKLLRVLQERKVRPVGAHEETSIDT
                     RVIAATNRDLAAMVKQGGFREDLFYRISVIPIELPPLRERREDIPTLIEHFVQKFCAQ
                     TGRSLGITEGATELLERYSWPGNVRELEHTIERSVALERTDTIQADSLPQSVTNYSPS
                     RAASSAFELPDEGLNLVAHLEHLEKSYLLEALRRTGGNQTRAAEVLHLSVRSLRHLLD
                     KHGIRSLTAQLRSDAASSTRGGAE"
     gene            6221..6712
                     /locus_tag="VF240_16930"
     CDS             6221..6712
                     /locus_tag="VF240_16930"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7176948.1"
                     /translation="MTQKKGQKGFSLIELLIVVAIIGIIAAIAVPNLLSSRRAANEGS
                     AQSSVRTIHSANATYQATTGNGEYGDVGDLLAEKLIDPVLGTGAKSGYTFESTPVASA
                     PATGDLAKFHATGDPTTDSGVSQSGTRNFCIAEDGVMRGGVGTSKAASRAACDALTAM
                     GNQ"
     gene            6884..7690
                     /locus_tag="VF240_16935"
     CDS             6884..7690
                     /locus_tag="VF240_16935"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7176949.1"
                     /translation="MSSRERRPNSWRRIMAIARNTFRESVRDKVLYNLVFFVLLLTGA
                     AIFLGDLSAGEEFKIIADLGLSATLLFGVFIAIFVGVGLVYKEIERRTIYSIFSKPVG
                     RGEFLVGKYIGLCLTLLVNLLVMGAGVTLALMYVRRPHNPSVLYVWPALLLIYAELTI
                     ITAAALLFSSFSSPSLSALLSFLLFIIGHFSADLKAFAVSLGTQGAKVFFTTLYYLLP
                     NLANYSFITPASYGHAPEPRALATSLLYAVAYNVALLAAASLIFRRRNFK"
     gene            7708..8649
                     /locus_tag="VF240_16940"
     CDS             7708..8649
                     /locus_tag="VF240_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176950.1"
                     /translation="MDDRGEGGATARAERRTLLALVAAVLFGLGSAAALTRWSESLRP
                     VAGAPEVADELYLKPETARRLSLNFNGLVADWYWMRSLQYVGRKVLAYEGQLQLDDLS
                     PLKLKVLAPLLEQTTTLDPHFYAAYEYGAVVLPAVDREAAVRLVQKGIDANPREWRLY
                     HQLGYIHWQGGQFKEAARVYEEGAQQSDAPTWMRAMPARLEAEGGDRSLAREMFTRLH
                     NEAGDEQIKELAIKRLAQLRSLDERDAIRRVLAIYRERALRCPESWAAIAPVLSRVPV
                     LRLGPDGAPLDPTDKPYVLTAGGCEVELGLGSEILRQ"
     gene            8698..9642
                     /locus_tag="VF240_16945"
     CDS             8698..9642
                     /locus_tag="VF240_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176951.1"
                     /translation="MTETVIQIENLTKDYEVGFLRKRRVRALDSLTVAVGRGEIFGFL
                     GANGAGKTTTLKLLMRLMYPTAGGATILGRDIGDVAMHARIGYLPEQPYFYDYLTARE
                     FLEYSAELFGYARDERRRRAAEVLARVRLDEKAWDRQLRKFSKGMLQRVGLAQSIVND
                     PEIVFLDEPMSGLDPVGRREVRDLIASLRDEGKTVFFCSHILSDIEVLCDRAAVLRKG
                     RLAHVGRLEELRQMGGERAMELIVAGADVVRLESELEGIEGARVTATPGGARVEVPSE
                     RQVDAALSAVRIAGARLVSIQPVGNPLEELFVNDEAGA"
     gene            complement(9798..10532)
                     /locus_tag="VF240_16950"
     CDS             complement(9798..10532)
                     /locus_tag="VF240_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7176952.1"
                     /translation="MTRCSKCEREVDAGQNFCPYCAEPLTATGREQLEQAARSSGAMQ
                     SAGQGRERFLRDSDLEPPSVVPLSSFDERRGETEKSQRKLFVVVGAASAVVLAGLVLL
                     FSTAAGKSLMERLTGAPPPDRLEGAARAGSPEFDQNVQKIFVDFNADDDATTAARPIG
                     DTVISMKPTLRNFTNRTINGLELRAAGYDASGQVVKARNFVIIPGRQSELEPNKTMSP
                     SLVLEGVKQGEMPASLRIEVTAFRFK"
     gene            complement(10572..12911)
                     /locus_tag="VF240_16955"
     CDS             complement(10572..12911)
                     /locus_tag="VF240_16955"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7176953.1"
                     /translation="MIDARTRLLVMLAVLSLPLLIISLFQLHSYQRNLGEQSRATAQF
                     ESASAARALEEWIAANHDTAAQPSPLGEGAAGSLYEYLHRHGAEGASFVVYDPRGEIV
                     PNPSAGGTPAHPQRSEARQVTEGRWSDGVKRVTGYGRAEPEGWGVAVGIPVPENTPAG
                     RDILMLTATWALTLFASSLLAWWAVGRFTKPLEKLTVTAASLGEGNLEERAEVETQDE
                     VGSLAASFNSMAANLQDKFEELQRQGAFISEVLDSLPLGVVVLDAGMTVRKANTTFAR
                     FVGRDASTLAGCGLYEAAAGLAVLSEVVEDVRRTRRAFVNYGQSLELVAPREGEDEGP
                     KHWDVILWPVTEQSGGPGSLILILSEVSKRVRAEKLASAAFAAEKSRAGELESVINQM
                     DEGVVIVDARGRYRVNPAGRRILGREPSEFRDGVEALNEDMGLRRVEGTALAPDDTPI
                     CRAFNKGEHVPAERLKIVRGDGEERVLAVGATPLVGEGGRRDGAASVFRDITDEVRHH
                     EELVAAYDRLREHDRLKSAFVANMSHELRTPLNVIIGLSQILQRDPQMPLAPLQDDAI
                     TRVERNARALLDLVNDMLNYSRLEAGRSALRIEPVNVAEVVESVAEEFAHEVGEKKLK
                     VRAEVSPELGAIRTDRQRLRQVLSCLVSNAIKFTSTGTIVVSAAPVDEARWYIEVSDT
                     GIGMTSDALTYIFDGFRQVDDRLARSYSGVGLGLAIARKIVELLDGEITVESRPGEGS
                     RFRITWPREVRMRTGTGSLVTDDARVVSDIADWRARMNR"
     gene            complement(12938..13477)
                     /locus_tag="VF240_16960"
     CDS             complement(12938..13477)
                     /locus_tag="VF240_16960"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HEX7176954.1"
                     /translation="MPERFLPLFREHADSLIRAWADAVYADSRTDLPVLLSYRELVEH
                     LPELFDELANVLDEAAQYELIVEATRRLRFHAQVRFQQGCLMDEVARELFLLRDALGD
                     FLWREGLDAADGDIRKLRDALRRANVFVDELISQTILVFASSLRPSVPTRRSTWPPPR
                     RRKTDFPEREEHEGDERKR"
     gene            complement(13613..14005)
                     /locus_tag="VF240_16965"
     CDS             complement(13613..14005)
                     /locus_tag="VF240_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7176955.1"
                     /translation="MVGVEKDVKTVLVVEDFEDNRFMMRRLLEMSGYRVIEAVNGQEA
                     VEKACAERPDLILMDLSLPHLDGLAATRRIRQYDGLGKVPIVAVSAHDTADFHADALA
                     AGCNEYVTKPIDFDQLENLLSRLVPQET"
     gene            14226..14885
                     /locus_tag="VF240_16970"
     CDS             14226..14885
                     /locus_tag="VF240_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176956.1"
                     /translation="MKPLTVDLQDEESLRDLGSASVQVVHDLKNQLNGLKLYATFLRK
                     RLEKGERPADELETVGKLIAGLERAANDMNALVRLGRPLDLHRQPHTDLSRILSAASE
                     GQKVETEAGAYEGEFDPAALTEALKGVGEGARVMLEQSRQTGALAIHLRREDTEGAPL
                     AVIEWRGAARREEGGADAFKSFAGPAGMRLALAARVIRAHGGEVAHEADILRARLPLS
                     T"
     gene            14996..16384
                     /locus_tag="VF240_16975"
     CDS             14996..16384
                     /locus_tag="VF240_16975"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7176957.1"
                     /translation="MERKEVRILVVDDEAMMSDSLKQNLEEEGYTADTASNGAEAIEQ
                     FDRGGHHLVICDLQLPDIDGIEVLRHAKDTSPSTEVIVMTGYGTVARAVEATKAGAFW
                     FLEKPFDFDVLQPLVEKAMERRDLMSQTETMRRQLATRTEYFNIIGSSKSMQTIYETI
                     ESVAKSDANVLIVGESGTGKELIANAIHYNSLRAKKPFIKVNCAALPKELIESELFGH
                     TKGAFTGAHADKDGLIQHAAGGSLLLDEIAEMPVELQPKLLRVLQERSYRKIGSERTY
                     AVDFRLISSTNRPPADAIRDGALRDDLFYRISTITIHVPPLRDRSEDIQLLAEHFLKM
                     YARKYQRPINGISQTAYQRLFGHTWPGNVRELQNVLERAVLLAKGNKIEPVDLPFENG
                     ALPEKGGQAAGWDVPPNMTLEDIEKLVIERTLQRTGGNKQAAANLLGIYRPRLYSKIR
                     KYKIDVPALVAS"
     gene            16485..18242
                     /locus_tag="VF240_16980"
     CDS             16485..18242
                     /locus_tag="VF240_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176958.1"
                     /translation="MLILRMHDLKRFARYFRPYKKSLVLGTLCILASVAAGLLVPGLV
                     GRAIDDLRQRPTWGAMTSAALVIVAVKIVSGVFLFLQRRILIGMSRHVEYDLRQDFYA
                     HLQRLPLSFFQSMRTGDLMARATNDLGAVRQLAGPMIMYTLQTVFVVVIVLPMMFLIS
                     VRLTLLLLLTMPLVSLTVKYFGQQVHVRFEKIQEFFSHISARAQENFSGVRVVRAYAQ
                     EDAEVAAFDDLNRQYAERNLSLVRISAVMRPLLTFFIGLGYVLIIWYGGRLAVRGEIT
                     VGQVVEFNLYLTTLIWPLIALGYVANLYQRGTASLKRMNAIFAIEPAIDDRAGVKEQP
                     AVRGRIEFRNLTFAYTEHSEPVLRDIDLTIEAGQTVAFVGRTGSGKSTLMNLIPRIVE
                     APPDTVFVDGVSVRDYPLAQLRSAIGYVPQETFLFSETLAENIAFGVEKSVRAEVEWA
                     AEIAGLSDDVREFPDGFDTMVGERGLTLSGGQKQRTAIARAVLRQPRILILDDALSSV
                     DTYTEEKILAQLRGVMRERTSLIVSHRVSTVRDADLICVLSEGRIVERGTHEELLAAG
                     GEYAELYELQLLEEELAAS"
     gene            18276..20138
                     /locus_tag="VF240_16985"
     CDS             18276..20138
                     /locus_tag="VF240_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7176959.1"
                     /translation="MATAVQQFHEEEAIDKAYDRAHSRRLLRYLRPYRRLIIPALLLT
                     LLANGLGVIQPKFFQWAIDWYIVPGNLSGLGLIAFAFLAVKVLQLFVSYLQTVFLQSV
                     GQHVMYDLRSELYRKLQRQEVAYFDRNPVGRVMTRLTSDVDALNELFTSGVIEGLGDV
                     VTIFAIVGFMLWMDWRLTLVALVTVPLLFAATVWFRKHARNGYDLVRTRLARINAFLQ
                     EHLSGAAVVQLFNRERKSFTQFHDVNDDHRRAHIETIFYYSVFFPLVDLIGALGVALI
                     IWYGGWRVMGASTSPGEQALTIGALVAFIQYSQQLFQPIRDISDKYNVLQSAIVASHR
                     IFKTLDLPVAVTTPNAPKKTGRAQGRVEFRNVWFAYKEEDWVLKDVSFAVEPGQSIAL
                     VGHTGSGKTTITNLLMRFYDVQRGQVLLDGVDVREWDLKHLRENFAVVLQDVFLFSGT
                     VEGNIRLGREEIAAERVEWAAREVRAHDFISRLPGGYKSEVRERGAGLSVGQKQLVSF
                     ARALAFDPAILILDEATSSIDTETEQLIQQAVERVLRDRTSLVVAHRLSTVQRADRIV
                     VLHHGEIREQGTHQELLTLRGLYWRLYKLQYADGARVRGAHPPGLPEGRMQYGD"
BASE COUNT         3532 a         6980 c         6181 g         3500 t
ORIGIN      
        1 gtcttgagga tgctcaacga gatcggctcc gtcgaacgcg tgcccgagta catgaagcgc
       61 gtgaaggccg gcgagttccg tctgatgggc ttcggccacc gcgtctacaa gaactacgac
      121 ccgcgcgcgc gcatcatcaa gcagatcgcc tacgaggtct tcgaggtgac gggcaaagac
      181 ccgctcttag acctcgcgct cgaattagag cgcatcgcgc tcgaagacga gtacttcgtc
      241 aagcgccgcc tctacccgaa cgtggacttc tactcgggga tcatctatcg cgccatgcgc
      301 ttccccgtgg acatgttccc cgtcttgttc gccatcccgc gcacctcggg ctggctcgcg
      361 cagtgggccg agatgctcgg cgaccccgac cagaagatcg cccgcccacg ccaaatctac
      421 ctcggccagc gcacgcgcga ctatgtgccc atggagaagc gcaacgggca ggccgagggc
      481 gccgccgctt cataagacgc tgacaacatt ctatgggcgt ggcatgtccg cggcctgtct
      541 tcgggaaaga attattaaca tggatggtca ggatgcacgg gataagatga tttgaaatct
      601 caaatcttga atcagagatt gagttttccc tcttcatcct gttactcctg tccatccatg
      661 taaattccaa aaattcgata gaacgaacag cggggagaac acatgctacc gacacgcgga
      721 tacgccaccg acggcccaac gtctcaactt caacccttcg acttcgagcg gcgtgagccg
      781 gggccgcgcg acgtgctcat cgaaatccag tattgcggag tctgccactc cgacatccat
      841 caggcgcgca acgagtgggg caactccatc tacccgatgg tgcccggcca tgagatcgtc
      901 gggcgcgtcg cgcgcgtcgg cgcgcaggtg tcgcgcttca aagagggcga cctcgccggc
      961 gtcggctgct tcgtggactc gtgccgcgtc tgcgccaact gcaaggaagg cgaggatcac
     1021 tactgcgaga atcatctcgt cgccacctac aacgggacgg agatggacga gaagacgccg
     1081 acctacgggg gctactcttc gcaggtggtc gtggacgaag actacacgct caaggttccg
     1141 gcgagtcttc cactccccaa cgtcgccccg ctgctctgcg ccggcatcac gacttactca
     1201 ccgctgcggc gcttcaaggt cgggccgggt cagcgcgtcg gcgtcgtggg cttgggcggt
     1261 ctgggacaca tgggtgtaaa gctcgccgcc tcgatgggcg caggcgtcac ggtcttcagc
     1321 acctccccgt cgaaggagtc ggacgcgcgc tcgctcggcg cgcacgactt cgtcgtcacg
     1381 aaagaccccg agaaccttgt ggcgctggcc ggcaagttcg acttcatcct cgacacggtg
     1441 tcggcggccc acgacctcaa catgtatctc gccctgctgc gccgcgaggc ggtgatggtg
     1501 ctcgtcggcg gccccgaaaa gcctctggag gtgcaagcct tctcgctcat caccaacggc
     1561 cggacgctcg ccggctcgat gatcggcggc atccgcgaga cacaggagat gctcgactac
     1621 tgcgccgaac acaacatcac ctcggacatc gagctgattc ccatcgggcg aatcgacgag
     1681 gcttacgagc gcaccgtcaa gggagacgtg cgctaccgct tcgtcatcga tatggaatcg
     1741 ctgaaaggca agtgagcagt aagcagtgag cagtaagcag taaaaacaaa gaccgcgccc
     1801 ccacatctga gcgggacgcg cggacgggaa caagtaaggc agccggagca tgacgcgccg
     1861 tgctgcctta cttgttttta ctgctcactg ctcactgctt ttcagaactt cccgaggagc
     1921 atcagcacgc cgatgacgat gaaggcgacg gccgcgacgc gcttgatcca ttcgagcggg
     1981 aggtagtggc cgagtgtgcc gccgacgagc acgccgacgg ccgagacgca gatgagcgcc
     2041 agcgacgccc cgacgaagac cgtccacgga cgcttcgtct gcgcggccat cgtcatcgcc
     2101 gccagttgcg tcttgtcgcc catctccgcc agaaagatcg tcccgaaggt cgccagaagt
     2161 acgcgccagt ccatcgcctt tgcacccgcc tttcgttttt tttcagacag tatgagcgcg
     2221 ccccgccttt tgtgctaggc tactgcgcga tgcttcagcg ggccgaaccg tcgcagagag
     2281 tttcgggcgc gcacgcgcga ctctggtggc tgatcgccgg gcggattgtc gccgccctcc
     2341 tcctgctggg cgcgagcgtc gtctggacgc gcttcttcct gcaggacggc atcgccgtgg
     2401 cgggcgcgcg cgcgcccgtg ccgcgcggcg cgctgcccat cggcgcggcc gtcctcgtcc
     2461 tctccttagt ctatgcggcg gcgcttcggc tcacgcgcct gagcgtcgtc gttcaggccg
     2521 gcgcacagtt cttctgcgac gccctgctcg tcacctggct cgtctcccag acgggcgacc
     2581 tccattcgcc ctacgccgcg ctctacatcc tcgtcatcgc catcgccagc ttctacctcg
     2641 gcgcgcgcga ggtgatgctc gtggccgccg ggtgcgccct gttctacacg gcggcgatgc
     2701 tctccatctc gctcggcttc gtgagcgcgc gcggggccga gaccgccgcg atgccgctcg
     2761 ccgaagtgct cgggacgatg ggactcaacg ccgccgtctg cctcgtcgtc ggcctgctgg
     2821 cggcgcgtct ggccgaccgg cagacgcgct ccgacgtgca gctccggcag accgcgcacg
     2881 cgctcgcctc cctgcgccgc ctgcacgagc ggatcgtcga ttcgatccgc tcgggcgtca
     2941 tcacgaccga cctctcgcgg cgcatctaca cggtgaacac ggccgccgag gagatgatcg
     3001 gctacggcgc ggccgacctg cgcgggcagg acgtgtcgat cctcttcggt gacatccgcg
     3061 cgcagatcga acagtctctg cgcgccgccg ccggcggtca caccagcccg cgcttcgagg
     3121 ccgactgctt gacggctgag ggctttcgcg tccgcctcgg ctacagcgtc tcgccgctct
     3181 cgggcgaggc cggcgacacc acagggttag tcatcacctt tcaggatttg acggacgtgc
     3241 gcgcgatgga ggagacctcg cgccggcagg agcgcctctc cgccgtgggc cgcgtcgccg
     3301 ccggcatcgc gcacgaaatc cgcaacccgc tcgccgccat gcgcggctcc atccaggttt
     3361 tgcgcgacga cctcgccgac gaccccggcc ggcaggagtt gatggagatc atcctgcgcg
     3421 agtccgaccg actcaaccgc atcatcaccg acttcctgac ctacgcgcgc ccccgacagg
     3481 tctcgctcgc cgccgttgac ctgcgcgagc cgctgcgcga aaccttcgcg ctactgcgcc
     3541 acagccccga gatgcgcccc gaccacacgc tcgaagaggt cttgcccgat gagccggtcg
     3601 cggcgtcggc cgacgaaaac gccatcaaac aggtcttctg gaacctctcg cgcaacgccc
     3661 tgatcgcgat gcccgagggc ggctccttgc gcgccgagat ggagacgctg ccgcagggcc
     3721 gcgtccgcat caccttcacg gactcggggc gcggcatgtc gcgctcgcag gtcgagcgcc
     3781 tcttcgagcc gttctcctcc tcgacgaccg gcggcaccgg cctcggcctc tcgattgtct
     3841 atcagataat ccgcgaccac ggtggtacaa tcaacgtccg cagccgcgag ggacatggga
     3901 cgacgatcac cgtggagttg ccgggtgcga agccttgagc cgcggccagg gtttcgcgat
     3961 gctcaaaccg aggagggaac gatgaccggc gaagagatgc aaaaggcgat ggatttcgtc
     4021 gttagtcaac aggcgcaatt cgcatccgac atcggcgagt taaaagatgt cgtggcacgg
     4081 ctggcgaacg cgaccctgag ccgcttcgag gccaccgacc ggaagcgcga cgacgttgac
     4141 cggcgcatct ccgcgcttgt ggactctcag ttgcggacgg aagagagtat tcgtaaaacg
     4201 gaagagaaca tcagtaggac ggaggataac gtccgagcgt tgactgctgt cgttgatcgc
     4261 tacttcagtg agggccgcaa cggcaaatag ctttcgcaag ttttcccctc acgatttccg
     4321 ctatggctaa acttctaatc gttgacgacg aacagggcat gcggcagctt ttgtcgctcg
     4381 tcttcgggcg cgcgggacac agtgtgcgcg cggccgagag cgggcggcgc gccgtcgaga
     4441 tgctgcgctc cgagccggcc gacgtgatcg tctcggacgt gaagatgccg gacatgtcag
     4501 gcatcgagat gctgcgcgcc gtgcgcgaat tcctgcccga cgcggccgtc gtgatgatga
     4561 cggccttcgc caccgtcgag acggcccgcg aggcgttcaa actcggcgcg gacgacttca
     4621 tccagaagcc cttcgacgtt gacgaactca agctcatcgt cgagaaggcg ctcgaacgcc
     4681 tccgtctcct tcaggagaac cgcgccttca agcgcgccca gcgcgagcgc ggccggctcg
     4741 accagatcat cggccactcg ccgcgcatgc aggccgtcta ccagatgatc gagacggtgg
     4801 ccgccgtcca gtcaaccatc ctgattacgg gcgagtcggg cacgggcaag gagatggtcg
     4861 cgcgcgccat ccacaacctc agcccgcgct ccgataagcc tttcatgccc atcaactgcg
     4921 gcgctttcac cgagacgctt ttggagtcgg agctgttcgg ctacacgcgc ggcgccttca
     4981 ccggcgccaa tgccaatcgc aaggggctgt tcgaggccgc cgacaagggc acgatttttc
     5041 tcgacgaggt cggcgagatg tcgccgacga tgcaggtcaa gctgctgaga gtccttcagg
     5101 agcgcaaggt gcggcccgtc ggcgcgcacg aggagacctc aatcgacacc cgcgtcatcg
     5161 ccgccaccaa ccgcgacctc gccgcgatgg tcaagcaggg aggcttccgc gaagacctct
     5221 tctaccgcat ctccgtcatc ccgatagagc ttcccccttt gcgcgagcgc cgcgaggaca
     5281 tccccacgct catcgagcac ttcgtccaga agttctgcgc gcagaccggc cgctcgctcg
     5341 gcatcacgga gggcgcgacc gagttgctcg aacgctactc ctggccgggc aacgtgcgcg
     5401 aactcgaaca caccatcgag cgctccgtgg cgctggaacg caccgacacc attcaggccg
     5461 actcgctccc gcaatcggtg accaactata gcccctcgcg cgccgcctcc tcggccttcg
     5521 agctgcccga cgagggcctc aacctcgtgg cccacctcga acatctggag aagagttacc
     5581 tcctcgaagc cctgcgccgc acgggcggca accagacgcg cgcggccgaa gtcctccacc
     5641 tctccgtccg tagcctgcgt cacctcctcg acaagcacgg catccgcagt ctcacggccc
     5701 aactccgctc cgacgccgcc tcgtccacgc gcggcggcgc ggaataagtc ggccgcacgc
     5761 gcggcgctga cggccgtcgg cgacggtcac gtccgtcagc catcgacggc acccggcttg
     5821 catagctttc gcgtgcgggg actcactcct ccgcctcaca ctctcacaat tttttctgga
     5881 acgttctggt aggcttttct ggaacgcgcg gcacgacttc cggccgccgg gccgggccgc
     5941 gtctttgaca aaatgcggca ccgctcgtct gacgattttt gttaaagcga cagttttccg
     6001 cccgaccgct ggtgtcactc acgaacctca cgccgcggcc acccttgcaa atattccagc
     6061 cgattagcgc ctcccgcgcc ggccccgccc tctggcacga cgcttgtaga aacacacggc
     6121 gacagaatcg cgcaacgcgg cgcgttgccc aagacgacga cgacccgaag ggcgcgagac
     6181 aaaccaaaac ctcaaacaag ttcaaggagc actcagaaca atgacccaga agaaaggcca
     6241 gaagggattc tccctcatcg aactgctgat cgtcgtcgcg atcattggca tcatcgccgc
     6301 catcgcggtt cccaatctcc tctcctcccg ccgcgcggcc aatgaaggct cggcgcagtc
     6361 ctccgtccgc accatccaca gcgccaacgc cacctatcag gccaccaccg gcaacggcga
     6421 atacggcgat gtcggcgacc tgctcgccga gaagctgatc gacccggtgc tgggcactgg
     6481 cgcgaagagc ggctacacct tcgagtccac ccccgtcgcc tccgcccccg cgaccggcga
     6541 cctcgcgaag ttccacgcga ccggcgaccc cacgaccgac tcgggcgtct cgcagtccgg
     6601 cacccgcaac ttctgcatcg cggaagacgg cgtgatgcgc ggcggcgtcg gcacctcgaa
     6661 ggccgcgagc cgcgccgcct gcgacgctct cacggccatg ggcaaccagt aagacttcgg
     6721 ttagccaatt cggaaaccgc gggggacgga gttcacgctc tgtcccccgc tctacgtgcc
     6781 ggggcgcgcg ccgcgcgcca cgcggcgaat acgggaaggt cgtatgttgg aaactgacgg
     6841 cgtgttaaaa ttcgcggcgc tgagagagga ggctcaaccc accgtgagtt ctcgagagcg
     6901 ccgcccgaat tcatggcgac gcatcatggc catcgcgcgc aacaccttcc gcgagtcggt
     6961 gcgcgacaag gtgctctaca acctcgtctt cttcgtgctg ctgctcacgg gcgccgccat
     7021 cttcctcggc gacctgtcgg cgggcgagga gttcaagatc atcgccgacc tcggcctgag
     7081 cgcgacgctt ctgttcggcg tcttcatcgc gatcttcgtc ggcgtcggtc tggtctacaa
     7141 ggagatcgag cggcgcacga tttattcgat cttctcgaag ccggtcgggc gcggcgagtt
     7201 tctcgtgggc aagtacatcg gcctgtgcct gacgctgctg gtcaacctcc tcgtgatggg
     7261 cgccggcgtg acgctggcgc tcatgtacgt gaggcgtccg cacaatcctt ccgtcctcta
     7321 cgtctggccg gcgctcctgc tcatctatgc cgagctgacc atcatcacgg ccgccgcgct
     7381 cctcttctca tccttctcct cgccttcgct ctcggcgctg ctctccttcc tcctgttcat
     7441 catcggacac ttcagcgccg acctcaaagc cttcgccgtc tcgctcggca cgcagggcgc
     7501 gaaggtgttc ttcaccactc tctactacct cctgcccaac ctcgccaact acagcttcat
     7561 cacgcccgcc tcctacgggc atgcccccga gccgagggct ttggccacct cactactcta
     7621 cgccgtcgcc tacaacgtcg ccctgctggc ggccgcgtcc ctcatcttcc ggcgtcgtaa
     7681 tttcaaatga gcgcgcgaga caacacgttg gacgacaggg gagaaggagg agcgacggcg
     7741 cgcgccgagc gccggacgct gctcgcgctc gtcgccgcag tcctctttgg actcggctcg
     7801 gccgccgccc tcacgcgttg gagcgagagc ctccggcccg tcgcaggcgc gccggaagtc
     7861 gccgacgagt tgtacctcaa gccggagaca gcccggcgct tgagtctcaa cttcaatggc
     7921 ctcgtcgccg actggtactg gatgcgctcg ctccagtacg tcggccggaa agtgctcgct
     7981 tacgagggcc aacttcagct cgacgactta agccctttaa agctcaaagt gctcgccccg
     8041 ctgctcgaac agacgaccac gctcgacccg catttctacg cggcatacga gtatggcgcg
     8101 gtcgtcctgc cggccgtaga ccgcgaggcc gccgtccgcc tcgtccaaaa aggcatcgac
     8161 gccaacccgc gggagtggcg tctctatcat cagctcggct acatccactg gcagggcgga
     8221 cagttcaaag aggctgcccg cgtctacgaa gagggcgcac agcaatcgga cgcgccgacg
     8281 tggatgaggg cgatgccggc gcgcctcgaa gccgagggcg gcgaccgcag ccttgcacgc
     8341 gagatgttca cacgcttgca caacgaggcg ggcgatgagc agatcaagga gctggccatc
     8401 aagagactgg cgcagctccg ctcgctcgac gagcgcgacg ccatccgccg cgtgctcgcc
     8461 atctaccgcg agcgcgcgct ccgctgcccc gagtcgtggg ccgccatcgc gccggtcttg
     8521 agtcgcgtcc ccgttctcag gctcgggccg gacggcgcgc cgctcgaccc gacagacaaa
     8581 ccttacgtcc tcaccgcggg cggttgcgaa gtcgaactcg gcctcgggtc ggaaattctc
     8641 cgacagtaag cacgaggccg ccgcgcccga cacttcagcg gaagggattc ctgagcgatg
     8701 accgagacag ttatccagat tgagaacctg accaaagact acgaggtggg cttcctgcgg
     8761 aagcggcgcg tgcgcgcgct cgactcgctc acggtcgccg tcgggcgcgg ggaaatcttc
     8821 ggcttcctcg gcgccaacgg cgccgggaag acgacgacgc tgaagctcct gatgcgtctg
     8881 atgtacccga cggcgggcgg cgccacgatc ctcggacgcg acatcggcga cgtggccatg
     8941 cacgcgcgca tcggctacct gcccgagcag ccatacttct acgactacct cacggcccgc
     9001 gagttcctcg aatactcggc cgagctgttc ggctacgcgc gcgacgagcg gcggcggcgt
     9061 gctgccgaag tgctcgcgcg cgtccgcctc gatgagaagg cgtgggatcg ccagttgcga
     9121 aagttctcga aggggatgct tcagagggtc ggtctcgccc agtcaatcgt caacgacccc
     9181 gaaatcgtct tcctcgacga gccgatgtcg ggcctcgacc ccgtcggccg ccgcgaagtg
     9241 cgcgacctca tcgcgagctt gcgcgacgag ggcaagaccg tcttcttctg ctcgcacatc
     9301 ctctccgaca tcgaagtgct ctgcgaccgc gcggccgtcc tgcgtaaagg ccgactcgcg
     9361 cacgtcggcc ggctcgaaga gctgcggcag atgggcggcg aacgcgcaat ggagctgatc
     9421 gtggcgggcg cggacgtggt caggctggag tcggagcttg agggcatcga gggcgcgcgc
     9481 gtgacggcca cgccgggcgg cgcgcgcgtc gaagtcccga gcgagcggca ggtggacgcg
     9541 gcgctcagcg ccgtccgtat cgcgggcgca cgcctcgtct ctatccagcc cgtcggcaac
     9601 ccgctcgaag agctgttcgt caacgacgag gccggcgcct gattcaacgt catgccggga
     9661 gcgcggtcat cccgacgact caaacagtac cgcgagcggt agcgagcggg tgcggtcttc
     9721 caagaccaga acccgctcgc taccgctcgc ggtactgttt gtcgctgata gccggcgtgc
     9781 gcccatgctc ccggcgttta cttgaatctg aacgccgtga cttcgatgcg aaggctcgcg
     9841 ggcatctcgc cctgcttgac gccttcgagg acgagcgagg gcgacatggt cttgttcggc
     9901 tcaagctcgc tctgcctgcc gggaatgatg acgaagttgc gcgccttgac gacctgcccg
     9961 gaggcgtcgt agccggcggc gcgcagctcc agcccgttga tggtgcggtt ggtgaagttg
    10021 cggagggtcg gcttcatcga gatgacggtg tcgccgatgg gccgggcggc ggtcgtggcg
    10081 tcgtcgtcgg cgttgaagtc aacgaagatt ttttgaacgt tctgatcgaa ctcgggcgag
    10141 ccggcgcggg cggcgccttc gagacggtcg gggggcggcg ctcccgtgag gcgctccatc
    10201 agcgacttgc ccgcggccgt cgagaacagc agcaccagcc ccgcgagcac cacggccgag
    10261 gccgcgccga cgacgacgaa cagcttacgc tgcgacttct ccgtctcccc tcggcgctcg
    10321 tcgaacgagg agagcgggac gacgctcggc ggctctaaat cagaatcgcg gaggaatctt
    10381 tcgcgcccct gccccgccga ctgcatggcc cccgaagagc gtgcggcctg ctccaactgc
    10441 tcgcggccgg tggccgttaa aggctccgcg cagtaagggc agaagttctg cccggcgtcc
    10501 acttcccttt cgcatttgct gcaacgcgtc acggccgttt aagtcctcct ctccgccggc
    10561 aagaagttac atcacctgtt catacgcgcg cgccagtcgg caatgtcgga gacgacgcgc
    10621 gcgtcatcgg tgacgagcga gccggtgccc gtgcgcatgc gcacctcgcg cggccaggtg
    10681 atgcggaagc ggctgccctc tccggggcgg ctctcgaccg tgatctcgcc gtcgagcagt
    10741 tcgacgatct tccgcgcaat cgcgaggccg aggccgacgc cgctgtagga gcgcgcgagc
    10801 cggtcgtcca cctgccggaa gccgtcaaaa atgtaggtga gcgcgtcgct cgtcatgccg
    10861 atgccggtgt cactcacctc gatgtaccaa cgcgcctcgt caaccggcgc ggcgctgacg
    10921 acgatggtgc cggtcgaggt gaacttgatg gcgttcgaga ccagacacga gagcacctgc
    10981 cggagcctct gccggtcggt gcgaatggcg cccagctccg gcgagacttc ggcgcgcacc
    11041 ttcagcttct tctcgcccac ctcgtgcgcg aactcttcgg ccacggattc taccacctcc
    11101 gcgacgttca ccggctcgat gcgcagggcc gagcggcccg cttcgaggcg cgagtagttg
    11161 agcatgtcgt tgacgaggtc gagcagggcg cgcgcgttgc gctccacgcg cgtgatggcg
    11221 tcgtcctgaa gcggcgcgag cggcatctgc gggtcgcgct ggagaatctg cgacaggccg
    11281 atgatgacgt tcaagggcgt gcgtagctcg tggctcatgt tggcgacgaa ggccgacttc
    11341 aggcggtcgt gctcgcgcag ccggtcgtag gccgcgacca actcctcgtg gtgtctgacc
    11401 tcgtcggtga tgtcgcggaa gaccgaggcc gcgccgtcgc gccggccgcc ctcgccgacg
    11461 agcggcgtgg cgccgaccgc cagcacgcgc tcctcgccgt cgcccctcac gatcttcagg
    11521 cgctccgcag ggacgtgctc gcccttattg aacgctcggc agattggcgt gtcgtcgggc
    11581 gccagcgccg tgccctcgac tctacgcagt cccatgtctt cgttcagcgc ctcgacaccg
    11641 tcgcggaact ccgacggctc gcgcccgaga attctgcggc ccgccgggtt gacgcggtag
    11701 cggccgcgcg cgtccacgat gacgacgccc tcgtccatct ggttaatcac gctctccagc
    11761 tcaccggcgc gcgacttctc ggcggcgaag gcggccgagg ccagcttctc ggcgcgcacg
    11821 cgcttggaga cctccgagag gatgaggatt agactaccgg ggccgcccga ttgttccgtc
    11881 accggccaca gaattacgtc ccagtgcttc ggcccctcgt cctcgccctc gcgcggcgcg
    11941 acgagttcga gcgactgccc gtagttaacg aaggcgcggc gagtgcgccg cacgtcctcg
    12001 accacttcac tgaggacggc cagaccggct gccgcctcgt acagaccgca gcccgccagc
    12061 gtcgaggcgt cgcgtccgac gaagcgcgcg aacgtcgtgt tggccttgcg cacggtcatc
    12121 ccggcgtcga gcacgacaac gccgaggggc agactgtcga gcacttcgct gatgaaagcg
    12181 ccctgccgct gcaactcctc aaatttgtcc tgaaggttcg ccgccatcga gttgaagctc
    12241 gcggcgagtg agccgacttc gtcctgcgtc tcgacctccg cgcgctcctc aaggtttccc
    12301 tcgccgagcg aggcggccgt cacggtgagt ttctccagcg gcttcgtaaa gcggccgacc
    12361 gcccaccacg ccagcaggct cgacgcgaag agggtgagcg cccacgtcgc cgtcagcatg
    12421 aggatgtcgc ggcccgcggg cgtgttctcc ggcacgggga tgccgacagc cactccccac
    12481 ccctccggct ccgcgcgccc gtagcccgtc acacgcttca cgccgtcgct ccagcgtccc
    12541 tccgtgacct gccgcgcttc ggagcgttgc gggtgcgcgg gcgtcccgcc ggccgaaggg
    12601 ttgggcacga tttcgccgcg tgggtcgtag acgacgaagg acgcgccctc agccccgtgc
    12661 cggtgcagat actcatagag ggagccggcg gcgccctcgc ctaagggcga gggttgcgcg
    12721 gctgtgtcgt ggttggcggc gatccactct tcgagggcgc gcgcggccga cgccgactcg
    12781 aactgggccg tcgcacgcga ctgctcgccg aggttgcgct ggtagctatg cagttggaaa
    12841 aggctgatga tgagcagtgg cagagacagg acggcgagca tcaccagcaa ccgcgtgcgc
    12901 gcgtcgatca taactagtgt ctctgccttt cttgtcatca gcgtttacgc tcatcgccct
    12961 catgctcttc gcgctccggg aagtcggtct tgcgtcgccg tggcggcggc catgttgatc
    13021 tcctcgtcgg cacgcttggc cttaaagaag acgcaaatac cagaatcgtc tgcgaaatca
    13081 gttcatcaac aaaaacgttg gcgcggcgca gcgcgtcgcg cagctttcgg atatccccgt
    13141 ccgcggcgtc aagcccctcg cgccacagaa aatcgcccag tgcatcgcgt aacagaaaca
    13201 actcgcgtgc cacttcgtcc atcaggcagc cctgttgaaa cctcacctgc gcgtgaaatc
    13261 gcagccgccg cgtggcctcg acgatcaact catactgcgc cgcctcgtcg agcacgttcg
    13321 ccaactcgtc gaacaactcc ggcaggtgtt cgaccaactc cctgtacgag agcaacacgg
    13381 gcaggtcggt gcggctgtcg gcgtagaccg cgtcggccca cgcgcggatg agcgagtcgg
    13441 cgtgttcccg gaagaggggc aggaaccttt cgggcataga gtgaaagccg tcaatcgtca
    13501 atcgtcaatc gtcaatcgtg gttgagcggt gcccaacttg tgtggcacgt cgcgagtcgc
    13561 ggggcgggca atcttagcga ttgacgattg acgattgacg attcacggcc ctttacgtct
    13621 cttgcggaac gaggcggctc aggaggtttt cgagctggtc gaagtcgatg ggcttggtga
    13681 cgtactcgtt gcagccggcg gcgagcgcat cggcgtggaa gtctgccgtg tcgtgcgcgg
    13741 agacggcgac gatgggcact ttcccgaggc cgtcgtactg gcgtatgcgg cgcgtggcgg
    13801 cgaggccgtc cagatgcggc aggctcaaat ccatcaggat caggtcgggg cgctccgcac
    13861 acgccttttc cacggcctcc tgaccgttga ccgcctcgat cacacggtag ccgctcattt
    13921 cgagtaaacg cctcatcata aagcggttgt cctcaaaatc ctcgaccacc agcacggtct
    13981 tcacgtcctt ttctactccc accataaccc cgctcgcttc caccaagttc tgcttgaaaa
    14041 cacgctcgca cgcgcccccg gctgacgtat gactagcaaa cagttccctt ttttgttatt
    14101 attccgcatc gaaatacaac acaaaaattg gcacagtttg agtttaatcc gtacatactg
    14161 cgaggtacgt ccggcccgtt agacgggtcg gaccgaacac ggaagctcgc gggttccgga
    14221 gctagatgaa gcctttaacc gtagatttac aggacgaaga gtctctccgc gacctcggca
    14281 gcgcttccgt gcaggttgtc catgacctca aaaaccaatt gaacggtctc aaactctatg
    14341 ccactttcct gcgcaagcgt ctggagaagg gcgagcgccc ggccgacgaa ctggagaccg
    14401 tgggcaagct catcgccggg ctggaacgcg cggccaatga catgaacgcg ctcgtccgcc
    14461 tgggccgccc gctggacttg caccggcagc ctcatacgga cctctcgcga atcctgagcg
    14521 ccgcgagcga aggtcagaag gtggagacgg aggccggcgc ctatgagggc gagtttgatc
    14581 cggcggcgct cacggaggcg ctgaagggcg tcggcgaggg cgcgcgggtg atgttggaac
    14641 agtcaagaca gacgggcgcg ctcgccatcc acctccggcg tgaagacacg gagggggcgc
    14701 ctttggcggt catcgagtgg cgcggggcgg ccaggcgtga agagggaggc gccgacgcct
    14761 tcaagtcctt cgccggcccg gcgggtatgc gcctcgctct cgccgcccgc gtcatccgcg
    14821 cccacggcgg agaggtggcg cacgaggccg acatcctgcg cgcgcgcctg ccgctcagta
    14881 catgaggaag tcaggagtca gaagtcagga gtcagaaata aaaacagcgg gtcgttttta
    14941 ttctgactcc tgactcgcgg actcctgact ccattcttct aaagggggag cagaagtgga
    15001 acggaaagaa gtgcgaatcc tggtggtgga cgacgaggcg atgatgtccg actcgctcaa
    15061 gcagaatctt gaggaggaag gctacaccgc cgacacggcc tcgaacggcg cagaggccat
    15121 cgaacaattc gaccgtggcg gccatcacct cgtcatttgc gatctccagt tgcccgacat
    15181 cgacggcata gaggtgctcc gacacgcgaa ggacaccagt ccctcgaccg aggtcatcgt
    15241 gatgacgggc tacggcacgg tggcgcgcgc cgtcgaggcg acgaaggccg gagccttctg
    15301 gttccttgag aagcccttcg acttcgacgt gctccagccg ctcgtcgaga aggcgatgga
    15361 gcgccgcgac ctgatgtcgc agaccgagac catgcgccgc cagctcgcca cgcgcaccga
    15421 atacttcaac atcatcggct cctcgaagtc gatgcaaacc atctacgaga ccatcgagtc
    15481 ggtcgccaag tcggacgcca acgtcctcat cgtcggcgag tcgggcacgg gcaaggagct
    15541 aatcgccaac gccatccact acaactccct gcgcgccaag aagcccttca tcaaggtcaa
    15601 ctgcgccgcg cttcccaaag agctgatcga gtcggaactc ttcggccaca ccaagggggc
    15661 gtttaccggc gcgcacgccg acaaggacgg actcatccag cacgcggcgg gcgggtcact
    15721 gctcctcgac gagatcgccg agatgcccgt cgaactccag ccgaaacttt tgcgcgtcct
    15781 acaggagcgc agctaccgca agatcggctc ggagcgcacc tacgccgtgg acttccgtct
    15841 catctcctcg accaaccgtc cccccgccga cgccatccgc gacggcgcgc ttcgcgacga
    15901 cctcttttac cgtatctcga ccatcaccat tcatgttccg cccctccgcg accgcagcga
    15961 agacatccag ctcctcgccg agcacttcct gaagatgtac gcgcgcaagt atcagcggcc
    16021 catcaacggc atctcgcaga cggcctacca gcgtctgttc ggccacacgt ggccgggcaa
    16081 cgtgcgcgag ctacagaacg ttttagagcg cgccgtgctg ctcgccaagg gcaacaagat
    16141 cgagccggtg gatttgcctt tcgagaacgg cgcgcttccc gaaaagggcg ggcaggccgc
    16201 cggctgggac gtgccgccca acatgacgct cgaagacatc gagaagctcg tcatcgagcg
    16261 cacgctccag cgcaccggcg gcaacaagca ggcggcggcc aatctcctcg gcatctaccg
    16321 cccgcgcctc tacagcaaaa tccgcaagta caagatcgac gtgcccgcgc tcgtcgcatc
    16381 ttaaagagct gagcatcgga cgacgaaggc ccgcccggca cggcgggcct tttgttgtgg
    16441 gcgcgcgtgc gcgtttggct ttcggctcgt tcatcccgca tcatatgctc atccttcgta
    16501 tgcacgactt aaagagattc gcgcgctact tccgccctta taaaaaatcc cttgttctgg
    16561 gcacactctg catcctcgcc tcggtggccg ctgggctgct cgtgcccggc ctcgtcgggc
    16621 gcgccatcga cgacctgcgc cagcgtccga cgtggggcgc gatgacgagc gcggcgctcg
    16681 tcatcgtcgc cgtcaagatc gtcagcggcg tcttcctctt cctccagcgg cgcatcctga
    16741 tcggcatgtc gcgccacgtc gagtacgatc tgcggcagga cttctacgcg cacctccaac
    16801 gcctgccgct ctccttcttc cagtcgatgc gcacgggcga cctgatggcg cgcgccacca
    16861 acgacctcgg cgccgtccgg cagctcgccg ggccgatgat catgtacacg cttcagaccg
    16921 tgttcgtcgt ggtcatcgtc ctgcccatga tgtttctcat cagcgttcgc ctcacgctgc
    16981 tgctcctgct gacgatgccg ctcgtctcgc tcaccgtgaa atacttcggt cagcaggtac
    17041 acgtccgctt cgagaagatt caggaattct tctcacacat ctcggcccgc gcgcaggaga
    17101 acttctccgg cgtccgcgtg gtgcgcgctt acgcgcagga agacgccgaa gtcgcggcct
    17161 tcgacgacct caaccgccag tacgccgagc gcaacctgag tctggtacgc atctcggccg
    17221 tgatgcgccc gctgctcacg ttcttcatcg gccttggtta tgtgctcatc atctggtacg
    17281 gcggccggct cgccgtgcgc ggcgagatca cggtcgggca ggtcgtcgag ttcaacctct
    17341 acctgacgac tctcatctgg ccgcttatcg cgctcggcta cgtcgccaac ctctaccagc
    17401 gcggcacggc gagcctcaag cgcatgaacg ccatcttcgc catcgagccg gcgattgacg
    17461 accgcgccgg cgtcaaggag cagcccgccg tgcgcgggcg catcgagttt cgcaacctga
    17521 cgttcgccta cacggagcac tccgagccgg ttttgcgcga catcgacctg accatcgagg
    17581 ccgggcagac ggtcgccttc gtcgggcgca ccggctccgg caaatcgacc ctgatgaacc
    17641 tcatcccgcg catcgtcgaa gcacctcccg acacggtctt cgtggacggc gtctccgtgc
    17701 gcgactaccc gctcgcgcaa ctccgcagcg ccatcggtta cgtcccgcag gagaccttct
    17761 tgttcagcga gacgctcgcc gagaacatcg cctttggggt ggaaaagtcg gtacgcgccg
    17821 aggtcgagtg ggcggccgag atcgccggac tctctgacga cgtgcgcgag ttccccgacg
    17881 gcttcgacac gatggtgggc gagcgcggcc tgacgctctc aggcgggcag aagcagcgca
    17941 cggccatcgc ccgcgccgtc ctccgacagc cgcgcatcct gatcctcgac gacgcgctct
    18001 cctccgtgga cacctacacc gaggagaaaa tcctcgcgca actgcgcggc gtgatgcgcg
    18061 agcgcacgag cctcatcgtc tcgcaccgcg tctcgaccgt gcgcgacgcc gacctcatct
    18121 gcgtcctctc tgagggccgc atcgtcgagc gcggcacgca cgaagaactg ctcgccgccg
    18181 gcggcgagta cgccgagctg tacgagctgc aactgctcga agaggaactg gcggcgagct
    18241 gaggcgcaac taaaaaggag gggttttaga aagaaatggc gacggcggtt caacagttcc
    18301 acgaagaaga ggccatcgac aaggcatacg accgcgcgca ctcgcgccgg ctcctgcgct
    18361 atctgcgccc ctaccgccgg ctcatcatcc ccgcgctact gctcacactc ctcgccaacg
    18421 gcctcggcgt catccagccc aaatttttcc agtgggccat cgactggtac atcgtccccg
    18481 gcaatctctc gggcttgggc ctcatcgcct tcgccttcct cgccgtcaag gtgctccagc
    18541 tcttcgtctc ttacctccag acggtcttcc tccagtcggt cggccagcac gtcatgtacg
    18601 acctgcggag cgaactctac cgcaagctcc agcggcagga ggtcgcctac ttcgaccgca
    18661 accccgtcgg ccgcgtgatg acgcgcctga cctcggacgt ggacgctctt aacgaactct
    18721 tcacctccgg cgtcatcgag ggtctcggcg acgtggtgac catcttcgcc atcgtcggct
    18781 tcatgctttg gatggactgg cgactgacgc tggtggcgct cgtcaccgtg ccgctcctgt
    18841 tcgccgccac ggtctggttc cgcaagcacg cgcgcaacgg ctacgacctc gtccgcacgc
    18901 gcctcgcgcg catcaacgcc ttccttcagg agcacctctc gggcgcggcc gtcgtgcagc
    18961 tcttcaaccg tgagcgcaag tcgttcacac agttccacga cgtgaacgac gaccaccgcc
    19021 gcgcgcacat cgagaccatc ttctactatt cggtcttctt cccgctcgtc gatctcatcg
    19081 gcgcgctcgg cgtcgcgctc atcatctggt acggcggctg gcgcgtgatg ggcgcatcca
    19141 cttcccccgg tgagcaggcc ctgaccatcg gcgcgctcgt cgccttcatc cagtactcgc
    19201 agcagctctt ccaacccatc cgcgacatct cggacaagta caacgttctc cagtcggcca
    19261 tcgtcgcctc gcaccgcatc ttcaagacgc tcgacctccc cgtcgccgtc accacgccca
    19321 acgcgccgaa gaagacgggg cgcgcgcagg ggcgcgtcga gttccgcaat gtctggttcg
    19381 cctacaagga agaagactgg gtgctgaagg atgtgtcctt cgccgtcgag ccgggccagt
    19441 caatcgcgct cgtcggccac accggctccg gcaagacgac gatcaccaac ctcctcatgc
    19501 gcttctacga cgtgcaacgc gggcaggtgt tactcgacgg cgttgacgtg cgcgagtggg
    19561 acttgaaaca cttgcgcgag aacttcgcgg tcgtccttca ggacgtgttt cttttcagcg
    19621 gcacggttga gggcaacatc cgtctggggc gtgaggagat tgcggccgag cgtgtcgagt
    19681 gggcggcgcg cgaggtgcgc gcgcacgact tcatctcgcg cttgcccggc ggctacaagt
    19741 cggaagtgcg cgagcgcgga gccggtctct cggtcggaca gaagcagctc gtcagcttcg
    19801 cccgcgcgct ggccttcgac cccgccattc tcatcctcga cgaagccacc agctccatcg
    19861 acaccgagac cgaacagctc atccagcagg ccgtcgagcg cgtgctgcgt gaccgcacct
    19921 cgctcgtcgt cgcgcaccgc ctctccaccg tccagcgcgc cgaccgcatc gtcgttctcc
    19981 atcacggcga aatccgcgag caaggaactc atcaggaact cctcaccctg cgcggtctct
    20041 actggcgtct ctacaaactg caatacgccg acggcgcgcg cgtgcgcgga gcgcatccgc
    20101 cgggcctgcc ggaagggcgc atgcagtacg gcgattgacg cacgggcaat cccttgaaag
    20161 cagaaagggc gggaactttc atgccccgcc ctt
//
LOCUS       DASVES010000413         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1239496, whole genome shotgun sequence.
ACCESSION   DASVES010000413 DASVES010000000
VERSION     DASVES010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239496"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VF240_16990"
     CDS             complement(<1..423)
                     /locus_tag="VF240_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7176960.1"
                     /translation="MRLADRTAFVTGGGRGIGRAVALAFAREGARVMVVARTAAEVER
                     VAGEIKSERGGDDASAHHATCDVSDPASVRAAFAVCAEAFGRPPDVLVNNAGIAESAP
                     VAKTSDEMWARHLAVNLSGVFYCTREALPPMVERGWGRV"
     gene            complement(524..1300)
                     /locus_tag="VF240_16995"
     CDS             complement(524..1300)
                     /locus_tag="VF240_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7176961.1"
                     /translation="MYDNIRVAHDAGIVTITLSRPEKLNAFAGHMRRDLAEALEHAGA
                     DASVRCVVVTGAGRAFCAGADVQFMSELMERDDVEEFRRLLGAGRRVLTTIRQMTKPV
                     VASVNGAAYGAGFNLALACDLRLAAESASFSQSFVKVGLHPDWGGTYFLPRVVPTNIA
                     CEMFFLGDAFDSQTALRFGLVNRVVSDAELETETRRLAERLRDAPRASVAAAKQAVYM
                     SEGESLERMIQYETEAQIQCFQSPEARKRVRAFLERKKER"
     gene            complement(1402..2247)
                     /locus_tag="VF240_17000"
     CDS             complement(1402..2247)
                     /locus_tag="VF240_17000"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7176962.1"
                     /translation="MAEAEAKVQQGAPAADASKELVRYEVREGVALLTLNDPPANTYS
                     YDMMRQLDRRVLEARMDDRVQVVVITGEGEKFFCAGANIAMLTEVTPTFKYYFCLHAN
                     ETLSRLEQTPKIVVAAINGHCVGGGLEVALAADLRVARRGAGRMGLPEINLGVLPGTG
                     GTQRLLRIVGKSRAIELLTTGELFDFERGRELGLVNQLFDADTSASFMEQALTYARQF
                     TTPGRAALAAGRIKRAVQTGAEIPFESALALERELQQQLFQSDDAKEGLAAYVEKRKP
                     EFKGR"
     gene            2460..2558
                     /locus_tag="VF240_17005"
     tRNA            2460..2558
                     /locus_tag="VF240_17005"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2494..2496,aa:Sec,seq:tca)
     gene            2672..3805
                     /gene="dprA"
                     /locus_tag="VF240_17010"
     CDS             2672..3805
                     /gene="dprA"
                     /locus_tag="VF240_17010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7176963.1"
                     /translation="MIQDWIALNMTPGVGPRAAAKLLERFGSAEGVFGALRAELARLR
                     LRPETVESIALKDRHEEAARELERVREIPGADVLVLDDGAYPALLREIADPPVTLYVR
                     GAWAECMEAPCAAVVGSRRASTYGQNVALMLARDLAARGVTVVSGLARGIDAAAHRGA
                     LEGAGRTVAVLGTGIDGVYPRDHRKLADEILERGGALVSQFPLETPPVSENFPYRNRI
                     ISGLSLGVCVVEAAENSGSLITARLAMEQNREVYAVPGNITSRNSFGTNYLIKSAGAK
                     LVQQWQDVVAEFPPEVAAAILPPEVKKKEAGAAGPASRVPNDLSGPERAVFKLLSTDD
                     PVHIDALAAASKLSISELSGVLLGLEMRELVRQLPGKCFVRKL"
     gene            complement(3972..5294)
                     /locus_tag="VF240_17015"
     CDS             complement(3972..5294)
                     /locus_tag="VF240_17015"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176964.1"
                     /translation="MDEMLRPGQAIRCEPSGMTCTVETLLGAGGQGEVYKAALGGESV
                     AVKWFFPQMGTAEQRAAIELLVRKGPPSEHFLWPIEITLAAGAPSFGYVMRLRPPQFK
                     NIVDLMTRRIEPTFRALATAGLNLSHNYLLLHSQGLCYRDISFGNAFFDPATGDILIA
                     DNDNVSVDGAGVLGVLGTPRFMAPEVVRGEAVPSTQTDLFSLSVLIFYMLVVAHPLEG
                     KKETEIKCLDLPAMTKLYGAEPLFIFDPADNSNAPVAGIHDNALAFWRIYPQFLRDIF
                     TRAFTDGIRDPLHGRVRESEWRGVMVRLRDSIFYCPHCSLENFYDGEALKARGGRAGL
                     CWSCATELRLPPRIRVGKSVVMLNHDAQLFPHHVDDQRLYDFSRSVAAVAAHPSNPGV
                     WGLKNLSPDKWVISTPDGAVKDVEPGRSATLAVGTKIRFGRAEGEIRT"
BASE COUNT          880 a         1744 c         1810 g          943 t
ORIGIN      
        1 gacgcgtccc cagccgcgct cgaccatcgg gggcagcgcc tcgcgcgtgc agtagaagac
       61 gccggagagg ttgacggcga ggtgccgcgc ccacatctcg tcgctcgtct tggcgacggg
      121 ggcgctctcg gcgatgccgg cgttgttgac gagcacatcg ggcgggcggc cgaaagcctc
      181 agcgcacacg gcgaaggcgg cgcgcacgct cgcggggtca gacacgtcgc acgtcgcgtg
      241 gtgcgccgaa gcgtcgtcgc cgccacgctc cgacttgatc tccccggcca cgcgctcgac
      301 ctcggccgcc gtgcgcgcca cgaccatcac gcgcgccccc tcacgcgcga acgccagcgc
      361 cacggcgcgt ccgatgccgc gcccgccgcc cgtcacgaac gccgtcctgt cagcaagtct
      421 catgatgaag aagtgatgag tgatgagtga tgagtgataa gaaaaggcag aagacggatg
      481 ctttctctta ttacttatca ctcatcactt atcacctgct ttcttacctc tccttcttgc
      541 gctcaaggaa cgcgcggaca cgcttgcgcg cctccggcga ctggaagcac tgtatctgcg
      601 cctcggtctc gtactgaatc atgcgctcaa ggctctcgcc ctcgctcatg tagacggcct
      661 gcttggccgc ggcgaccgag gcgcgcggcg cgtcgcgcag gcgctcggcg agacggcgcg
      721 tctccgtctc aagctcggcg tccgagacga cgcggttgac gaggccgaag cgcagcgcgg
      781 tctgagagtc gaaggcgtcg ccgaggaaga acatctcgca ggcgatgttg gtggggacga
      841 cgcgaggcag gaaatacgtg ccgccccagt ccgggtggag gccgaccttg acgaacgact
      901 ggctgaagga ggccgactcg gcggcgagcc gcaggtcgca ggcgagcgcg agattaaagc
      961 ccgcgccgta ggccgctccg ttgaccgagg cgacgaccgg cttggtcatc tgtctgatgg
     1021 tcgtcagcac gcgccggccc gcgccgagca gtcgccggaa ctcctccacg tcgtcgcgct
     1081 ccatcagctc cgacatgaac tgcacgtccg ccccggcgca gaacgcccgc cccgcgcccg
     1141 tcacgaccac gcagcgcacc gaggcgtccg cgcccgcgtg ctccagcgcc tccgccagat
     1201 cgcgccgcat gtgccccgcg aacgcattca gcttctcggg ccggctcagc gtgatcgtca
     1261 cgatgcccgc gtcgtgcgcg actcggatgt tgtcgtacat aagtgacgag tgataagtga
     1321 tgagtgataa gtgataagag aaagactttg tcttctgcct tttcttatca cttatcactc
     1381 atcacttatt acctcccttt atcaccttcc tttaaattcc ggctttcgct tctcgacgta
     1441 ggcggcgagt ccttctttcg cgtcgtcgga ctggaagagt tgctgttgga gttcgcgctc
     1501 taaggcgagg gcggactcga aggggatttc ggcgccggtc tggacggcgc gcttgatgcg
     1561 gccggcggcg agggcggcgc gccccggcgt ggtgaactgg cgcgcgtagg tcagcgcttg
     1621 ctccatgaag gaggcgctgg tgtcggcgtc gaagagttgg ttgacgaggc cgagttcgcg
     1681 gccgcgctcg aagtcgaaca gctcgcccgt cgtcagcaac tcgatggcgc gggacttgcc
     1741 gacgatacgg aggaggcgtt gcgtgccgcc ggtgccgggc aggacgccca gattgatctc
     1801 aggcaggccc atgcgaccgg cgccgcggcg ggcgacgcgg aggtcggcgg cgagggcgac
     1861 ttcgaggccg ccgccgacgc agtggccgtt gatggcggcg acgacgatct tcggcgtctg
     1921 ctccagacgc gacagggtct cgttggcgtg aaggcagaag tagtatttga aggtgggcgt
     1981 gacctccgtg agcatcgcga tgttcgcccc ggcgcagaag aacttctcgc cctcgcccgt
     2041 gatgacgacg acctgcacgc ggtcgtccat gcgcgcttcg agcacgcggc ggtcgagctg
     2101 gcgcatcatg tcgtaagagt atgtgttggc cggcgggtcg ttgagcgtca ggagcgcgac
     2161 gccctcgcgc acctcgtagc ggacgagttc tttcgacgcg tcggccgcgg gcgccccttg
     2221 ttgaaccttc gcttcagctt cagccatgac gtgttcggct ccgttggtcg tcggatatga
     2281 attgcgaaat tcgggatgta cggccgtggc ggcatgatag cgtgcgcgcc gacggcaggt
     2341 aaagcgcgcc ccgcgccggg gcacgcgttc ggtaagcgcg cacgatgcaa cggacgcgct
     2401 tgacagccgc gccgcgcgcc gcgtaggttc tttggcgttc gtccctcgcc acctcacgcg
     2461 gaggcgcatc tgttctggtg aacggcgcgg tcttcaaaac cgtctgcggg cctgtgagag
     2521 caggcccggg tgggttcgat tcccacacgc ctccgccaaa aaaaagtgag cagtgagcag
     2581 taagcagttg gccgcttcag cgactcgacg cttatcgccg aacctgtttt caactgctca
     2641 ctgcttactg ctcactgctc actctcctca catgattcaa gactggatcg ctcttaacat
     2701 gacgccgggc gtggggccgc gcgcggcggc gaagcttttg gaacgtttcg ggtcggccga
     2761 gggcgtgttc ggcgcgctgc gcgcggagct tgcgcggctg cggctgcggc ccgaaacggt
     2821 cgaatcaatc gcgctcaagg acaggcacga ggaggcggcg cgtgagctag agcgcgtgcg
     2881 cgaaatcccc ggcgccgacg tgctcgttct cgacgacggc gcttacccgg cgctcctgcg
     2941 cgagatcgcc gacccgcccg tcacgctcta cgtgcggggc gcgtgggccg agtgcatgga
     3001 ggcgccgtgc gcggcggtcg tcggctcgcg gcgcgcttcg acctacgggc agaacgtcgc
     3061 cttgatgctc gcgcgcgatc tggccgcgcg cggcgtgacg gtcgtctcgg gcctcgcccg
     3121 cggcatcgac gcggcggcgc atcgcggcgc gctcgaaggc gcggggcgca ccgtggctgt
     3181 tctcggcacc ggcatcgacg gcgtctaccc gcgcgaccac cgcaagctcg ccgatgagat
     3241 actcgaacgc ggcggcgcgc tcgtctcgca gtttcctctg gagacgccgc ccgtctcgga
     3301 gaatttcccc tatcgcaacc gcatcatcag cggcctctcg ctgggcgtct gtgtcgtcga
     3361 ggccgccgag aactccggct cgctcatcac ggccagactc gcgatggagc agaaccggga
     3421 ggtctacgcc gtccccggca acatcacttc gcgcaactcc ttcggcacga actacctcat
     3481 caagtcggcc ggcgctaagc tcgtgcaaca gtggcaggac gtggtcgccg agttcccgcc
     3541 cgaagtggcc gctgccatac tcccgccgga ggtgaagaag aaagaggccg gcgcggcggg
     3601 gccggcgtct cgcgtgccca atgatctttc ggggccggag cgcgcggtct tcaagctgct
     3661 ctcgacggac gaccccgtgc atatcgacgc gctcgccgcc gcgagtaagc tttccatctc
     3721 ggagttgtcg ggcgtgctgc tcggactgga gatgcgcgaa ctcgttcgcc aactccccgg
     3781 caaatgtttt gttcgcaaac tctgaaggca gtagcccgac cgtgagggag ggcgtatagc
     3841 tcccgcgtca tgcgccatcc ctcacggtcg ggctactgcc ttttgttcct gcgccctctg
     3901 gcctctgcgg tgaatctatt ccccgcttca ctcaactgaa catctctaca gtcgaaggcc
     3961 ggcgcgcgcc gtcacgtcct gatctcgcct tccgccctgc cgaaacgtat cttcgtgccc
     4021 acggcgaggg tggcgctgcg gcccggctcc acgtccttca ccgcgccgtc gggggtgctg
     4081 atgacccact tgtccggcga caaatttttg aggccccaca cgccggggtt cgacgggtgc
     4141 gcggccacgg cggcgacgct tcgggagaag tcgtacaacc tctggtcgtc aacgtgatgc
     4201 gggaagagct gcgcgtcgtg gttgagcatg acgaccgact tgccgacgcg gatgcggggc
     4261 gggagccgca gctccgtggc gcacgaccag cacagacccg cccggccgcc gcgcgccttc
     4321 agcgcctcgc cgtcgtagaa gttttcgagc gagcagtgag gacagtaaaa aatcgagtcg
     4381 cgcaggcgaa ccatcacgcc gcgccactcg ctctcgcgca cgcgtccgtg aagcggatcg
     4441 cgtatgccgt cggtaaaggc tctcgtgaag atgtcgcgca aaaactgcgg gtagatgcgc
     4501 cagaaggcga gcgcgttgtc gtggatgccg gcgacggggg cgttggagtt gtcggcaggg
     4561 tcgaagatga agagcggctc ggcgccgtag agtttggtca tcgccggcag gtcgagacac
     4621 ttgatctcgg tctccttctt cccttcgagc ggatgggcga cgacgagcat gtagaagatg
     4681 agcacggaga gcgagaagag gtcggtctgc gtggaaggga cggcttcgcc gcggacgacc
     4741 tcgggggcca tgaagcgcgg ggtgccgagc acgccgagca cgcctgcgcc gtccaccgag
     4801 acgttgtcgt tgtcggcgat gaggatgtcg ccggtggcgg ggtcgaagaa ggcgttgccg
     4861 aaggagatgt cgcggtaaca gagtccctgc gaatggagca gcaggtagtt gtgcgagagg
     4921 ttgaggccgg cggtggcgag cgcgcggaag gtcggctcga tgcgccgcgt catcaggtca
     4981 acgatgttct tgaattgtgg tggcctgagc cgcatcacat agccgaagct cggcgccccg
     5041 gccgcgagcg taatctcaat gggccagagg aagtgttcgc tgggcggccc cttgcggacg
     5101 agcagctcga tggccgcgcg ctgctcggcc gtccccatct gcgggaagaa ccatttcacg
     5161 gcgaccgact cgccgccgag cgccgccttg tagacttcgc cctgcccgcc cgcgccgagc
     5221 agcgtctcca ccgtgcaggt catccccgac ggctcgcagc ggatggcctg acccggcctt
     5281 agcatctcat ccatcaacgc ctcccgacgt aagagttcaa agttcaaagt tcaaagttca
     5341 aaggacgagc cgtcttcctg actttgaact ttgaact
//
LOCUS       DASVES010000414        13765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_812998, whole genome shotgun sequence.
ACCESSION   DASVES010000414 DASVES010000000
VERSION     DASVES010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812998"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1645
                     /locus_tag="VF240_17020"
     CDS             107..1645
                     /locus_tag="VF240_17020"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7176965.1"
                     /translation="MNTDTRDAGTATGAERARRSLDRFIFASLLALGFLVAAPYGAVD
                     PWWEGAFEASVFALGALWMLEGMLAGGGWVVPEQRLLLPVIALILFAAAQTLALAPAR
                     EVAGVLVGASLSADTQETWRFVIKLAALALALALLLRYTSGARRLRALVHAAAGVGFL
                     SAIFGVLRLVGGRETVAFVSGRLAANRDGFAQFINRNHFAFLAEMALGVALGLLIHAA
                     RRRRRLPAYAALALTVWTALVLSNSRGGILSMFVALISTALLYTSPAAKEREASESSS
                     AVRREEDETHGGRDAQRSRRGRRRRGWRARAGRDSWVFKGALLASLLVVTLVGVLWVG
                     GEKLAERIEALPTDVAAEGPRWGDRRAEIWRAAWQLFLDHPLTGVGFGAFRAAVPTHH
                     DASGEMSLEQAHNDYLELLASGGIFGAALFAWLIWMIVGRARRSLRSPDSFRRAAAAG
                     AAAGLLAVAAHSLFDFGLHVTANAYVLVMLIAVVCAGARVEGEPRKQAAAASRVRSRA
                     EEGD"
     gene            1653..2924
                     /locus_tag="VF240_17025"
     CDS             1653..2924
                     /locus_tag="VF240_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176966.1"
                     /translation="MFDRRRLLRHLLAAAFMLACVVGSRHAFRAGMSRLMAETTPRLS
                     VNTEGADALGVAGRAVELNASDPEAHDALAVALARRGEFDAAERALERAATLRPRYFR
                     QWQRLGRARTLTGYREGALAAYAEAVRLAPFYAEPRWQLGNELLRGGRTAEAFAELRR
                     AAESRPALAAYTAELAWRAYDGEARAVAGAVAPHTAQGRVALARFFARRGRESEALEQ
                     YRAGGAEAAEGERRSLVSDLIAAGKYRAAREVWAGDAAASSGADAPGIGDGGFESQSS
                     LAGAGFEWQFARDQPAVRFSLDSAAPRDGARSLLVTFGGDPDPSARLVTKLFTVEPGA
                     RYRLVFAARSEELVSAGLPVVALLDPADARVLAQAEPVPVKTDGWREYQIEFTTPADG
                     ALLIVRRSGCAVRTCPIFGRLWLDAFTLQKL"
     gene            3350..7606
                     /locus_tag="VF240_17030"
     CDS             3350..7606
                     /locus_tag="VF240_17030"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX7176967.1"
                     /translation="MPRKSAARHSVRSTTNFKLLSIVLLCAAVVLAALTSGAGASAQA
                     EACLTPTVQIITDPAGDQSGAPLANQSFDIRTISIAEDYRNFEFSRLVITMKVTSLTT
                     LPPNGAWRTRFTFGGVSYFVVMETDTSSAVRYEYGDFGGTGGSLRTLGTAVSGTNSAD
                     GNIVITVNSSNVGNAAAGQMLTAMSGATQQFVGTPEVGGGYLGMDSTTTTSTYTLAGK
                     PAACVMSTPTPTPTPTPGGGGGGVCNASGISATTDPAGDHNATIGTAQQDIREVLVAE
                     PYFTDGSSKLVFTVKVESLDPNNLPANAIWKVYFNVGTTTHFVAAFNDPATGASFDYG
                     AKDANGIDQTAGAADAGSFDGAAKSINIIVSNGKVGSPGAGQQLAAVTGTTQLLIGSG
                     EVGGSLQQVDTTTPGSYTLVGNAACAPDSTPTPTPTPTTGAGPEYTIYQPPSGMGQSA
                     GEPSVGINWKTGKVFFIASTQTLRVTFDDCVQPATAKWEDVSFPTTNRATLDPILYTD
                     SATGRTFVSQLLGKASSTVYTDNDAGVNGQGVGDWIQSQGSGINSGVDHQTLGGGPFA
                     APLTRDPADTTLYPNAVYYASQDAAVAQAAVSLDGGQTFGPAVPMYNLLQCGGIHGHL
                     KVAPDGTAYVPNKQCGSTQGVVVSENNGASWVVRPVTGSTASSGIKDPSVGIATDGRI
                     YFGGTDGNSLPFTAVSRDKGVTWTNLQRIGAGLGIVNATFPQMIAGDPDRAAFAFLGS
                     TTGGDGQSPITAGNPFKGAWHLYIATTYDGGVTWTTINATPGDPVQQGSICNSGTVVC
                     EREPNDRNLLDFNDIQIDKQGRPYAAYADGCITSSCINGDYRNNDYAARASIARQSGG
                     KTLFAAFDPTGATVPKSPAVTAQSDAAGVHLSWTPPDDGGSPITGYKVYRRAQGAPAP
                     TLLASLPATQLSYDDTTADPATTYLYHVRAVNIIGESDFCPGSEVVPSLVPDHCNLPG
                     VRVTSDPAGDTTPPPPVAPTSVDIRSVSVAEPFFGAGVNKLVITLKVGTGAAPASSQW
                     YVIWSRPPGTPDAATFDRNYVAMKTSATGAISYEYGKVAPPNANLPTRVGDADAGTFD
                     PATGTITITISNSKLDNVGVGQGLNTLDARSFWNRADGQPVTGLQATDTTPAGGYELV
                     GNAACEPNEAPVAVLTATPKSGLAPLNVAFNAAGSTDADGDAIVEYTFNFGDGASETR
                     TTSQLGDAAKTAAHTYTTPGSYRAFLSVKDAAGNSSLNVAEELITVNAGGCAVNWALA
                     SNGASASASSTSNSRDYSPANTIDGDNTGVGWEQGGGWNDDTRDLWPDWLDVNFNGAQ
                     AVNEIRVYTLQNDYSQPVVPDETTDASYYGISDFEVQAWDGTQFVTVPGGLITGNTKA
                     MRVITLGAPVTTSAIRIKVNAGRIHFSRIVEVEAIGCAAP"
     gene            7736..8350
                     /locus_tag="VF240_17035"
     CDS             7736..8350
                     /locus_tag="VF240_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176968.1"
                     /translation="MVGKLSATVLLCLFLFQSDGCGSRSGGGNQNSKPSATQATPTSA
                     PAASPGVAQSPAGACLLIDKSEIESLQGEQMRGAEPSTGTEADMAFSDCYYTVISSDG
                     SKNLSVHLRVLRDDPQSAGRGALRRFWEDRFRGAKEKKKAEKPRVVAGVGEEAFWLGN
                     NVMGALYVLAKDRIVRVSVGGAEEEDAKIEKSRKLAEKAINHLD"
     gene            8878..13743
                     /locus_tag="VF240_17040"
     CDS             8878..13743
                     /locus_tag="VF240_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX7176969.1"
                     /translation="MRNGRFIRATLIATTLASALVFSLNPSSAQLGGTVTVNTTAVSG
                     GGTQQQAMPKEFDVRGPDGVPKGTALRAPQPAQLTALASLGNSVGSRLSVQYNGLTAT
                     PRHLFSHGSYLTAPSAAAPETIARDFIAANRGLFRFSNSDLRNLRLKSRAVLPDMGTT
                     VLLFEQMVGGLSVYNGSVLVNVNRTGRIMSVGGESFPNLSITNAAAITPEQPLSDAYT
                     ITPEQAITNAAADLGVSGFTPQSAGTQQVPRNFGNLPYESETAPKFKRGVFTDDIVVT
                     KVIFPVGDQARLAYRFVLTTPQFSGIMWESIVDAQTGKVLRRISLTSFQQGGGTGVGR
                     RGTFRPDVQDLVEAHNAAGTAKGKVTDTTPTALSGYRGFGRSTAPGNAPTYGNESAGA
                     NDRLAGRGFKQSQVFARNENPLIYNTGFGQVVRGFPDATNPSAASPLGWFYLPTNTGG
                     TEIAAANDVRAATRAYGYTMSAEAKTRNLPANSPAGNGDQPFSATLTALGSPLALGDG
                     RTLTNVFQSNYTEGNNVNVADDRADDNEGSHGVRGYDANRQFTAAHFDYTMGYEYGGI
                     NAGGTPFYPASANPDVYPGAVTLFYYNNLIHDYLYSIGFTEATWNFQQDNFGKGGIGH
                     DGLSAQVQDGSGTNNANMSTAADGSAPRMQMYLFTEDVFRRSDGDFDFDVVAHEYYHG
                     VSNRSAAKGSSGCLGVAFFGESGGQGEGWSDTIAQSMTDDDSTGEFVTGEQDKGIRTI
                     PVTNFRWSYGAIDGRKLNRRDQMTADPVGAPGVGTGIPPTTPAYQVHHVGTVFSAMTW
                     DMRELLIMKQKVGGVFPGVFFDGTRRLGSGTSFFIGERQVQSVDTKHPIDYRDSFGTH
                     AIVSPTPNTTTVPPTLGTIPVVVPTINAADHIVRPGMVAGEFATNGNRQGPLSTAVSQ
                     GARLADTLILKGLQIAPCNPSIVDTRDGILLADKELTGGENRAVIWRAFASHGVGALA
                     ASSHSGAATDPASSMVPVIVEDFSVPASVLACEQQGPLAPPTFALTNPSDNTVQINVT
                     PVAGAARYIVSRSQNENGPFVQVAEGAGTSYTDSNLPAGAYFYQVRASRDTEFNCVST
                     ANTLSIAVTGVVIPPAPLFAGVDRVDDPQDGARLIVSWKPATSLNPTAQIVYDVFSVD
                     TVEQGDGTQNASFTPSPSNRIATGITGTSYNHTGLSLADVQYYIVQARDANNSTLDTN
                     NAGNTVTKFNAPTVAAVSGTPVFALETFETTAANSRFTPGLTESGGNPSTASATFQRV
                     VGEQFGGYGTAGKMFAPDFSPGHETSPGTCPTALSCGGASDYYTMIGGPAGLTLTSTS
                     ILEFDHSFNQEAFFDGGVLELKVGAPFVNGDATPFPDNVSVYDLANFTIEGNYNAPLD
                     GTLEATVKGSPLQGRLAYTGAKGLHHTRISLNDFAPGSTYNPQSLPVYIRFRNTSDVA
                     SSNGVDQGWYVDNVVINNLACSVNVAGASSGATATASSTQTSRNYSPAGAIDGERTGE
                     SWESGGGWNDDTRGLWPDTLTINFNGEQTISEVRVYTLQNDYHNPVEPTPLTPADYYG
                     ILDFEVQTCNGETCTTVPVTGTVTGNDKAMRIITLGTPINATGVRIKVNAGRVYFSRI
                     VEVEAFGCSAQ"
BASE COUNT         2431 a         4933 c         4275 g         2126 t
ORIGIN      
        1 gttacggcgc ggccggcagg cggcgcgtca gtgaagagtt cagcgccgaa gtgatgaccg
       61 cgcgcacgct cgacctctac cgcgaagtct caaactcgcc cgcccgatga acaccgacac
      121 gcgcgacgcc gggacagcta caggcgccga gcgggcgcgc cgctcactcg accgcttcat
      181 cttcgcgtcg ctgctcgcgc tcggctttct cgtcgcggcg ccctacggcg cggtcgatcc
      241 gtggtgggag ggagcgttcg aggcgtcggt cttcgcgctc ggggcgctgt ggatgctgga
      301 gggaatgctc gcgggtggcg gttgggtcgt gcccgagcaa cggctgctgc tccccgtcat
      361 cgccctgatt cttttcgcgg cggcgcagac tctcgcgctt gcgcccgcgc gcgaggtggc
      421 gggcgtgctg gttggcgcga gcctgagcgc cgacacgcag gagacgtggc gcttcgtcat
      481 taagctcgcg gcgctcgcgc tcgcgctcgc actcctgctg cgatacactt cgggcgcgcg
      541 ccgcctgcgc gcgctcgtcc acgcggcggc cggcgtcggg ttcctgagcg caatcttcgg
      601 cgtcctgcgt ctcgtcggcg ggcgcgagac ggtcgcgttt gtctccgggc gactcgccgc
      661 caaccgcgac ggcttcgcgc agttcatcaa ccgcaaccac ttcgccttcc tcgccgagat
      721 ggccctcggc gtcgcgctcg gactgctcat ccacgccgca aggcgacgcc gacgcctccc
      781 cgcatacgcc gctctggcgc tcaccgtctg gacggcgctc gtcctctcga actcgcgcgg
      841 cggcatcctc tcgatgttcg tcgccctcat ctcgaccgcg cttctgtaca cgtctccggc
      901 cgcgaaggaa cgagaagcgt ctgagtcttc gtcagccgtt agaagagaag aagatgagac
      961 gcatggagga cgagacgcgc aaaggtcgcg ccggggccgg agacgacgcg ggtggcgcgc
     1021 tcgcgccggg cgcgactcgt gggtctttaa gggtgcgctg ctcgcgtccc tgctcgtcgt
     1081 gacgctcgtc ggtgtgctgt gggtgggcgg cgagaagctg gccgaacgca tcgaggcgct
     1141 gccgacggac gtggctgccg aggggccgcg ctggggcgac cgccgcgcgg agatttggcg
     1201 cgcggcgtgg caactctttc tcgaccaccc gctcacgggc gtgggcttcg gcgcgtttcg
     1261 ggccgccgtc cccacacacc acgacgcctc cggcgagatg tcgctcgaac aggcgcacaa
     1321 cgactatctc gaactgctcg cgagcggcgg catcttcggc gcagcactct tcgcgtggct
     1381 catctggatg atcgtcggcc gcgcgcgccg ctccctgcgc tcgcccgact ccttccgacg
     1441 cgccgccgcg gccggagccg ccgctggcct tttggccgtc gccgcccaca gccttttcga
     1501 tttcggactc cacgtcaccg ccaacgcata cgtcctcgtg atgctcatcg ccgtcgtctg
     1561 cgctggcgct cgcgtcgaag gcgagccgcg taagcaagcg gcggccgcgt cgcgcgtgag
     1621 gtcgcgagcg gaggagggtg actgacgtgg agatgttcga caggcgacga ctattgaggc
     1681 atctgttagc cgccgccttc atgctcgcgt gcgtcgtggg gtcgcgtcac gccttccgcg
     1741 ccggcatgtc gcggctgatg gcggagacga cgccgcgcct gagcgtcaac accgagggcg
     1801 cggacgcttt gggagtcgcc gggcgagccg tcgaactcaa cgcgtccgac ccggaggcgc
     1861 acgatgcgct cgccgtcgcg ctcgcgcgtc gtggcgaatt cgacgcggcg gagcgcgcac
     1921 ttgagcgcgc cgccacactc cgcccgcgtt atttccgtca atggcaaagg ctggggcgag
     1981 cacgcacttt gacgggctat cgggaagggg cgctcgcggc atacgcggag gccgtgcgcc
     2041 tcgccccgtt ctacgccgag cctcgctggc agttgggcaa cgagctgctg cgcgggggga
     2101 gaaccgcgga ggctttcgcg gagctgcgcc gcgccgccga gagccggccg gcgcttgcag
     2161 cctacacggc cgagcttgcc tggcgcgcat acgatggcga agcgcgcgcg gtggccgggg
     2221 ccgtcgcgcc gcacacggcg caggggcgcg tggcgctcgc gcgcttcttt gcgagacgtg
     2281 ggcgcgaatc agaggcgcta gagcaatacc gcgcgggcgg cgcggaggcg gctgagggcg
     2341 agcggcgctc gctcgtgtca gatttgatcg cggcagggaa gtatcgcgcc gcgcgggagg
     2401 tgtgggcggg ggacgcggcg gcgtcgtcgg gcgcggacgc gccgggtatc ggcgacggcg
     2461 gcttcgagtc tcaaagcagt ctggccggtg cgggcttcga gtggcagttc gcgcgcgacc
     2521 agccggcggt caggttctcg ctcgactcgg ccgcgccgcg cgatggggcg cgcagcctgc
     2581 tcgtcacctt cggcggcgac cccgacccct cggcgcgtct cgtcaccaaa ctcttcacgg
     2641 tcgagccggg cgcgcgctac cgcctcgtct tcgccgcgcg ctccgaagaa ttagtcagcg
     2701 ccgggctgcc cgtggtcgcg ctcctcgacc ccgcggacgc tcgcgtgctc gcgcaggcgg
     2761 aacccgtacc cgttaagacc gacggctggc gcgagtatca aatcgagttc acgacccccg
     2821 ccgacggcgc gctcctcatc gtccgccggt cgggctgcgc ggtgaggacg tgccccatct
     2881 tcggccgcct ctggctcgac gctttcacct tgcagaagtt gtaacctcat acgaactcgc
     2941 ttgagtgaat gcagagtaga ttcaccacgg aggcgcagat gacgcagagt tcacgccgca
     3001 gggttgaatt ctctgcgccc cctctgcgtc ctctgcgcct ctgcggtgag tccaacatcc
     3061 catacactca actctgaaat ctgaaatacc cccacttcgg caccctatcc caagcctcaa
     3121 ccactccaca cgaaacccga cgtcgccgcc tgacgcttcg caagcgaagg gctaagtgtt
     3181 gaaataatta atcctgacga cactcccgcg cgcgtgagca ccgccgccga agaatatttt
     3241 tcgggcctca agtatttctc aaccgggtgg ggatattttc tgtctcgtaa tctggaaaca
     3301 caaagttttg accgaccaca accacgaaat ttcaatgagg agatgcgaca tgccccgtaa
     3361 atctgccgcc cggcacagtg ttcgttccac cacgaacttc aagcttcttt caatcgtgct
     3421 cctgtgcgcc gccgtcgtgc tcgccgcgct cacgtccggc gcgggcgcct ccgcgcaggc
     3481 cgaagcgtgc ctgacgccga ccgtgcagat catcaccgac ccggccggcg accagagcgg
     3541 ggcgccgctg gccaatcaga gttttgacat tcgcacgata tcgattgccg aggattacag
     3601 aaattttgaa ttcagcaggc tcgttattac gatgaaggtg acgagtctca ccacgctgcc
     3661 gccgaacggc gcgtggagaa cgcgcttcac cttcggcggg gtgagttatt tcgtcgtcat
     3721 ggaaaccgac acgagttcgg cggtcagata tgagtatggt gatttcggcg gcacgggcgg
     3781 ttctctccgc acattgggca ctgccgtctc cggcacgaac tccgcggacg gcaacatcgt
     3841 cattaccgtc aatagcagca acgtgggtaa cgcggcggcg ggccagatgc tcacggcgat
     3901 gagcggggcg actcagcaat tcgtcggcac ccccgaggtc ggcggcggct acttgggcat
     3961 ggattcgacg accaccacct ccacctacac gctggccggc aaaccggcag cctgcgtgat
     4021 gtcaacgccg accccgacgc cgacgccgac acccggcggc ggcggcggcg gggtgtgcaa
     4081 tgcgtctggc atatcggcca ccaccgaccc ggcgggcgac cacaacgcca ccatcggcac
     4141 ggcgcagcag gacatccgcg aagtgctcgt cgccgagcct tacttcaccg acggcagcag
     4201 caagctcgtc ttcaccgtga aggtcgaaag cctcgacccg aacaacctgc cggccaacgc
     4261 catctggaaa gtctacttca acgtgggaac cactactcac ttcgtcgccg ctttcaacga
     4321 cccggcgacg ggcgcctcct tcgattacgg cgcgaaggac gccaacggca tcgaccagac
     4381 ggcgggcgcc gccgacgccg gctcgttcga cggtgccgcc aaatcaatca acatcatcgt
     4441 ctccaacggc aaggtcggca gccccggcgc cggccagcag ctcgcggccg tcaccggcac
     4501 gacgcagttg ctcatcggct ccggcgaggt cggcggctcc ttgcagcagg tggacacgac
     4561 cacgcccggc agttacacgc tcgtcggcaa cgccgcgtgc gcgcccgact cgacgcccac
     4621 gccgactccg acgccgacca ccggcgcggg gccggagtac accatctatc agccgccgtc
     4681 aggcatgggc cagagcgccg gcgagccgtc cgtcggcatc aactggaaga cgggcaaggt
     4741 cttcttcatc gccagcacgc agacccttcg cgtcaccttc gacgactgcg tccaaccggc
     4801 cacggccaag tgggaagacg tttccttccc gacgaccaac cgtgccacgc tcgaccccat
     4861 cctctacacc gattcggcca cgggccgcac cttcgtctcg caactgctcg gcaaggccag
     4921 ctcgaccgtc tacaccgaca acgacgccgg agtgaacggc cagggcgtcg gcgactggat
     4981 acagagtcaa ggctccggca tcaactccgg cgtcgatcac cagacactcg gcggcggccc
     5041 gttcgccgcg ccgctcaccc gcgaccccgc ggacacgacg ctctacccga acgccgtcta
     5101 ctacgcctcg caggacgcgg ccgtggcgca ggcggccgtc agtctcgatg gcgggcagac
     5161 cttcggcccg gccgtcccga tgtataacct gctccagtgc ggcggcatcc acggccacct
     5221 caaggtcgcg cccgacggca cggcctacgt gcccaacaag cagtgcggct ccacgcaggg
     5281 cgtggtcgtc tccgagaaca acggcgcgtc gtgggtcgtg cgccccgtca ccggctcgac
     5341 cgcctcgtcc ggcatcaaag acccatcggt cggcatcgcc accgacggcc gcatctactt
     5401 cggcggcacc gacggcaact ctctgccctt cacggccgtc tcgcgcgaca agggcgtgac
     5461 gtggacgaac ctgcaacgca tcggcgcggg cctcggcatc gtcaacgcca ccttcccgca
     5521 gatgatcgcc ggcgaccccg accgcgccgc cttcgccttc ctcggctcga ccacgggcgg
     5581 cgacggccag tcgcccatca ccgccggcaa cccgttcaag ggcgcgtggc acctctacat
     5641 cgccacgacc tacgacggcg gcgtgacgtg gacgaccatc aacgccacgc cgggcgaccc
     5701 cgtccagcaa ggctccatct gcaactccgg cacggtcgtc tgcgaacgcg agccgaacga
     5761 ccgcaacctg ctcgacttca acgacattca aatcgacaag cagggccggc cctacgccgc
     5821 ctacgccgac ggctgcatca cctcgtcctg catcaacggc gactaccgca acaacgacta
     5881 cgcggcccgc gcctccatcg cccgtcagtc cggcggcaag accctgttcg cggccttcga
     5941 cccgaccggc gcgaccgtgc cgaaatcgcc cgccgtcacg gcccagagtg acgccgccgg
     6001 ggttcacctg agctggacgc cgcccgacga cggcggctcg cccatcacgg gctacaaagt
     6061 ctaccgccgc gctcagggtg cgcccgcgcc gaccctcctc gcgtcgctcc cggccacaca
     6121 gctcagctac gacgacacga cggccgaccc tgccacgacc tacctctatc acgtgagggc
     6181 ggtcaacatc atcggcgaga gcgacttctg ccccggcagc gaagtcgtcc cgtcgctcgt
     6241 gcctgaccac tgcaacctgc cgggcgtgcg cgtcacctcc gacccggccg gcgacaccac
     6301 gccgccgccg cccgtggccc cgacttccgt ggacattcgt tcggtctccg tcgccgagcc
     6361 tttcttcggc gcaggggtta ataaactggt catcaccctg aaggtgggga cgggcgccgc
     6421 gccggccagc agccagtggt acgtcatttg gagccgcccg ccgggcacgc ccgacgcggc
     6481 cacattcgac cgcaactacg tcgccatgaa gacgagcgcg acgggagcca tctcctacga
     6541 gtacggcaaa gtcgcgccgc ccaacgccaa cctgccgacg cgcgtgggcg acgccgacgc
     6601 cggaaccttc gacccggcga cggggacgat caccatcacc atctccaaca gcaagctgga
     6661 taacgtcggc gtcggtcagg gattgaacac gctggatgcc cggagcttct ggaaccgggc
     6721 cgacggtcag ccggtgacgg ggctgcaagc caccgacacc actccggcgg gcggctacga
     6781 gctggtgggc aacgccgcgt gcgagccgaa cgaagcgccc gtcgcggtct tgacggcgac
     6841 gccgaagtcc ggcctcgcgc cgctcaatgt cgctttcaac gccgccggct cgaccgacgc
     6901 cgacggcgac gccatcgtcg aatacacgtt caactttggc gacggcgcct ccgagacgcg
     6961 cacgacatcg cagctcggcg acgccgcgaa gacggccgcg cacacttaca cgacgccggg
     7021 cagctaccgc gcgttcctga gcgtcaagga cgccgccggc aactcgagcc tcaacgtggc
     7081 cgaagagttg atcacggtca acgccggcgg gtgcgccgtc aactgggcgc tcgcctccaa
     7141 cggcgcttcg gccagcgcct cgtccacctc caacagccgc gactactcgc ccgccaacac
     7201 catcgacggc gacaacaccg gcgtcggctg ggagcagggc ggcggctgga acgatgacac
     7261 gcgcgacctc tggcccgact ggctcgatgt taacttcaac ggcgcacagg ccgtcaacga
     7321 gattcgggtc tacaccttgc agaatgacta cagccaaccc gtcgtgcccg acgagacgac
     7381 cgacgccagc tactacggca tttccgactt cgaggtgcag gcgtgggacg gcacacagtt
     7441 cgtcaccgtg ccgggcggcc tcatcaccgg caacacgaag gccatgcgcg tcatcacgct
     7501 gggcgcgccc gtgacgactt cggccatccg catcaaggtc aacgcgggtc gcatccactt
     7561 ctcgcgcatc gtcgaggttg aggccatcgg ctgcgccgcg ccgtagccct gagccggaaa
     7621 gataaaggat gagggatgaa ggatgaggga tgaaggatga gggatgaaaa tcgcttccac
     7681 ttcatccctc atccctcatc cttcatccct caatttttca taagggagct tcccgatggt
     7741 cggcaaactc agcgccacag ttctgctctg ccttttcctc ttccagtctg acggctgcgg
     7801 ctccagaagc ggcggcggca atcagaactc gaagccgtct gccacacagg cgactcccac
     7861 gtccgccccg gccgccagtc ccggcgtggc gcagtcgccg gccggcgctt gtctgctaat
     7921 cgacaagtcc gaaatcgagt ctctacaggg cgagcagatg cgcggggccg agccgagcac
     7981 ggggacggaa gccgacatgg ccttctcgga ctgctactac acggtcatct cctccgacgg
     8041 gtcgaagaac ctctccgtcc acctccgtgt gttgcgcgac gacccgcaaa gcgcgggcag
     8101 gggtgcgctc aggcgtttct gggaggacag gttccgtggg gcgaaagaga agaagaaggc
     8161 ggagaagccg cgcgtggtcg ccggcgtcgg cgaggaagcc ttctggctcg gaaacaacgt
     8221 catgggggcg ctctacgtgc tggcgaaaga ccggatagtc cgcgttagcg tgggcggcgc
     8281 cgaagaggag gacgcgaaga tcgagaagtc ccgaaagtta gcagagaagg cgattaatca
     8341 cctcgattaa acggagtcca tgatgaaatt caaacttaat cgcaaaaccc gcatgctcaa
     8401 gcgacgggcc gccatcatgc ttatcgcgct ttggataatc ggcgcggtgt ttgcgatgac
     8461 catgctcttc agagcctgac gccgaagcct gtaggcgctg tccgcggccg ctcctcgcgc
     8521 cccgtgcgcg cgccccgccg gaaaagaatc gacgtgcgcc accgatgggg acggccgcac
     8581 ccgtttggtc acggggcgga agcgcccaag tgcccctctt gcgccgcctc aaaaactttt
     8641 ccagcgccgc gcgccaccgt ctcaagtcgc ctcaaccacc tgccgcgacc accccgcccg
     8701 catcccgcaa aatagttaag ttccactgtt cccgtagttt agcggacgct cacgggggcg
     8761 cgagcgtgac ctcaagtatt tctcaactcc ctcggattat tttctgtgac gttaagagcg
     8821 aaaagctcag ccgtgaatcc ccgagagggg cagccgccaa tctttaagga gtaaataatg
     8881 cgaaacggaa gatttatcag ggccacactc atcgcgacga ccctggcgtc cgcactggtc
     8941 ttcagcctca acccctcctc cgcgcaactc ggcggaaccg tcaccgtcaa caccacggcg
     9001 gtgagcggcg gcggcacgca gcagcaggcg atgcccaaag agttcgacgt gcgtggcccc
     9061 gacggcgtgc ccaaggggac ggccctgcgc gccccgcaac cggcccagtt gacggcgctc
     9121 gcttcgctcg gcaacagcgt cggctcccgt ctctcggtac agtacaacgg gctgacggcc
     9181 accccgcgcc acctcttcag ccacggcagc tatctgacgg cgcccagcgc ggcggccccc
     9241 gaaacaatcg cgcgcgactt catcgccgcc aaccgcggtc tcttccgctt cagcaacagc
     9301 gacctccgaa acctaagact caagagccgc gcggtgttgc ccgacatggg cacgaccgtt
     9361 ctgcttttcg agcagatggt gggcggcctg agcgtctata acggcagcgt gctcgtcaac
     9421 gtcaaccgca ccgggcgcat catgagcgtc ggcggcgaga gcttcccgaa cctctccatc
     9481 accaacgccg ccgccatcac gcccgagcag ccgctctccg acgcctacac catcactccc
     9541 gagcaggcca tcacgaacgc ggcggctgat ttgggcgtga gcggattcac accccagagc
     9601 gcggggacgc agcaggtgcc gcgcaacttc ggcaacctcc cttacgagtc cgagaccgcg
     9661 cccaagttta agcgcggcgt cttcaccgac gacatcgtcg tcaccaaggt catcttcccc
     9721 gtgggcgatc aggcgcgcct cgcctacagg ttcgtgctca cgacgccgca gttcagcggc
     9781 atcatgtggg aatcaatcgt ggacgcgcag acgggcaagg ttctgcgccg catcagcctg
     9841 acttcgttcc agcagggcgg cggcacgggc gtgggccgtc gcggcacctt ccgcccggac
     9901 gtgcaggacc tcgtcgaagc gcacaacgcg gcgggcacgg cgaaaggtaa agtgaccgac
     9961 accactccca ccgcgctttc cggctaccgc gggttcgggc gctccacggc tccgggcaac
    10021 gctccgacct acggcaacga gtctgccggg gcgaatgacc gcctcgcggg tcgcggcttc
    10081 aagcagagtc aggtcttcgc gcgcaacgaa aacccgctca tctacaacac cggcttcggt
    10141 caggtggtgc gcggcttccc cgacgccacc aacccttcgg ccgcctcgcc cctcggctgg
    10201 ttctacctgc cgacgaacac gggcggaacc gagatcgccg ccgccaacga cgttcgcgcc
    10261 gccacgcgcg cctacggcta caccatgtcg gccgaggcca agacgcgcaa cctgccggcc
    10321 aactcgccgg cgggcaacgg cgaccagccc ttctccgcca cgctcacggc gcttgggtct
    10381 cccctggcgc tcggcgacgg ccgcacgctc accaacgtct tccagtcgaa ctacaccgag
    10441 ggcaacaacg tcaacgtcgc cgacgaccgc gccgacgata acgaaggctc acacggcgtc
    10501 cgcggctacg acgccaaccg gcagttcacc gccgcccact tcgattacac gatgggttac
    10561 gagtacggcg gcatcaacgc cggcggcacg ccgttctacc cggcgtcggc caacccggac
    10621 gtgtaccccg gcgcggtgac gctcttctac tacaacaacc tcatccacga ttacctctac
    10681 agcatcggct tcaccgaggc gacgtggaac ttccagcagg acaacttcgg caagggcggc
    10741 atcggccacg atggtctgag cgcgcaggtg caggacggct ccggcaccaa caacgccaac
    10801 atgagcaccg cggccgacgg ctcggccccg cgcatgcaga tgtatctctt caccgaggac
    10861 gtgttccgtc gctccgacgg cgacttcgac ttcgacgtgg tggcgcacga gtactatcac
    10921 ggcgtctcga accgctcggc ggcgaagggt tcgtcgggct gtctcggcgt ggccttcttc
    10981 ggcgagtcgg gaggacaggg cgaaggctgg tccgacacca tcgcgcagtc gatgaccgac
    11041 gacgactcga ccggcgagtt tgtcaccggc gaacaggaca aaggcatccg caccatcccc
    11101 gtcaccaact tccgctggtc atacggcgcg attgacggca ggaaactcaa tcgccgcgac
    11161 cagatgacgg ccgaccccgt gggcgccccc ggcgtcggca ccggcatccc gccgaccacc
    11221 cccgcttatc aggtacacca cgtcggcacg gtcttcagcg cgatgacgtg ggacatgcgc
    11281 gagctgctca tcatgaagca gaaggtgggc ggcgtcttcc ccggcgtctt cttcgacggc
    11341 acgcgacgcc tcggctccgg cacgtcgttc ttcatcggcg agcgtcaggt gcagtcggtc
    11401 gataccaagc acccgattga ctaccgcgat tcgttcggca cgcacgccat cgtgtcgccg
    11461 acgcccaaca cgacgaccgt cccgcccacc ctcggaacca tcccggtggt cgtccccacg
    11521 atcaatgccg ccgatcacat cgtccgcccc ggcatggtcg cgggggagtt cgccactaac
    11581 ggaaaccggc aggggccgct ctcgaccgcg gtcagtcagg gcgcgcgtct cgccgacacg
    11641 ctgattctca aaggactcca gatcgccccg tgcaacccgt ccatcgtgga cacgcgcgac
    11701 ggcattctgc tggccgacaa ggaactgacc ggcggcgaga atcgcgccgt catctggcgg
    11761 gccttcgcca gccacggcgt cggcgcgctg gccgcctctt cacactccgg cgcggcgacc
    11821 gacccggcaa gctcgatggt tccggtcatc gtcgaagact tcagcgtccc ggcgagcgtg
    11881 ctcgcctgcg agcagcaggg gccgctcgcg ccgccgacct tcgcgctcac caacccgtcc
    11941 gacaacaccg tccagatcaa cgtcacgccg gtcgccgggg ccgcgcgtta catcgtctcg
    12001 cgcagccaga acgagaacgg gccgttcgtg caggtggccg agggcgcggg aacttcctac
    12061 accgacagca acctccccgc gggcgcctac ttctatcagg tgcgcgcgag ccgcgacacg
    12121 gaattcaact gcgtctccac cgccaacacg ctgagcatcg cggtgacggg cgtcgtgatt
    12181 cctccggccc cgcttttcgc cggcgtggat cgggttgacg acccgcagga cggcgcgcgc
    12241 ctgatcgtga gctggaagcc ggcaacgtcg ctcaatccga ctgctcagat cgtctacgac
    12301 gtgttcagcg tggacaccgt cgagcagggc gacggaaccc agaacgcttc gttcacacct
    12361 tccccgtcga accgcatcgc gacgggcatc accggcacgt cgtacaacca cacgggtctg
    12421 tctctggccg acgttcagta ctacatcgtc caggcccgcg acgcgaacaa cagcaccctt
    12481 gacacgaaca acgcgggcaa cacggtcacg aagttcaacg cgccgacagt cgcggccgtg
    12541 agcggcacgc cggtcttcgc gctggagacc ttcgagacga ccgccgcgaa cagccggttc
    12601 acgccggggt tgacggagtc gggcggaaac ccgagcacgg cgtcggcgac cttccagcgt
    12661 gtagtgggcg agcagttcgg cggctacggc acggccggca agatgttcgc gccggacttc
    12721 agccccggcc acgagacgag tcccggcacc tgcccgaccg cgctcagttg tggcggcgca
    12781 agcgactact acacgatgat cggtgggcct gccggcctca ctctgacttc gacttcaatt
    12841 ctggagtttg accactcgtt caatcaggaa gccttcttcg acggcggcgt gctcgaactc
    12901 aaagtgggcg ctcctttcgt caacggcgac gcgacgccgt tcccggacaa cgtcagcgtc
    12961 tacgacctcg ccaacttcac catcgagggc aactacaacg ccccgctcga cggcacgctg
    13021 gaggccacag taaagggttc accgctgcaa ggccgtctcg cttacacggg tgcgaagggc
    13081 ttgcaccaca cgcggatttc tctgaacgac tttgctccgg gcagcacgta taacccgcag
    13141 agcctcccgg tctacatccg cttccgcaac acctcggatg tggcctcgtc caacggtgtc
    13201 gatcagggct ggtacgttga caacgtggtg attaacaacc tcgcttgcag cgtcaacgtg
    13261 gccggcgcat cgagcggcgc gaccgccacc gcgtcctcga cccagacgag ccgcaactac
    13321 tcgcccgcag gcgccatcga cggcgaacgc acgggcgaga gctgggaatc gggcggcggc
    13381 tggaacgacg acacccgcgg cctctggccc gacacgctga ccatcaactt caacggcgag
    13441 cagaccatca gcgaggtgcg cgtctacacc ctgcaaaacg actaccataa cccggtggag
    13501 ccgacgcctc tgactccggc cgactactac ggcatcctcg acttcgaggt gcagacctgc
    13561 aacggagaga cctgcacgac ggttcccgtg acagggacgg tgacgggtaa cgacaaggcg
    13621 atgcggatca tcaccctggg gacgcccatc aacgccacgg gggtacgcat caaggtcaac
    13681 gcgggccgcg tctacttctc gcgcatcgtc gaggtcgagg cgttcggctg ctcggcgcag
    13741 tagattttta aagcggggcg tgcgg
//
LOCUS       DASVES010000415         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1292346, whole genome shotgun sequence.
ACCESSION   DASVES010000415 DASVES010000000
VERSION     DASVES010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292346"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VF240_17045"
     CDS             <1..876
                     /locus_tag="VF240_17045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7176970.1"
                     /translation="LLLLVIDSIFIPNFFHPNNFRNILLQIAPTMLVATGMTFVIATG
                     GIDLSVGAVMAIASAVAATSIDSGTWVAVPLALCVATAAGAFNGALISGFRIQPIIVT
                     LALLISGRGVAQVINGGRLIPFSDPTFEYLGRGYVGPVPVQVLVMFVVVAAAIFVMRA
                     TAFGRYVVAVGGNESAARLSGVKVNRTKVLVYALSGLLAGLAGLIETARQGASDAPNV
                     GLNIELDAIAATVVGGTALTGGRATVVGTLIGALIMQVITTSINMKGYEYAWSLVIKA
                     AIIVVAVYIQRPKVA"
     gene            933..>2483
                     /locus_tag="VF240_17050"
     CDS             933..>2483
                     /locus_tag="VF240_17050"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX7176971.1"
                     /translation="MSRHFQSPPRPTRKRLIRALGCVCLWAVAAAAQQAGPTYANPVI
                     AGDFPDPSVIRVGDDYWAMTTTGTWAPHFPILHSRDLVNWRVAGYVFRRKPAWVKSDF
                     WAPEITEDRGRFFAFYTARRDEGPKRVGTLCVAVATARRPEGPYTDRGPLVCEIRELR
                     NVGSIDAFFVRDERDVPHLVWKADGNDAEPDVPTSVFAQRLSADGTKLLGRRREILRN
                     TEGWERHVTEGSDIVRRNGWFYHFYSGNACCGRGCDYALGVARSRRLLGPWEKSRRNP
                     ILDDNSDWQCPGHGTIVTTADGRDFLLYHSYRRRRDTFNVGREALLDEVKWGADGWPT
                     INDGRGPTSLAASPHAAPVSDDEGEVFDDFAGPQLGLEWQWPMFFEQSARVEPSRGGR
                     LVVEIPKGARADEWTAAVLARRTTSGSYVATTQVDLRGTTAGARAGLSAYSWREWSVG
                     VAAGGGKIAVWRREGKDLRTLATADAPSAPAVQLRMTASGGETYRFAYSVDGREWTDV
                     GGPVSAGYI"
BASE COUNT          371 a          879 c          843 g          390 t
ORIGIN      
        1 ctgctgctgc tcgtcatcga ctcgatcttc atcccgaatt tcttccaccc gaacaacttc
       61 cgcaacatcc tcctccagat cgcgccgacc atgctcgtcg ccaccggcat gaccttcgtc
      121 atcgccacgg gcggcatcga cttgtcggtg ggcgcggtga tggcgattgc gtcggccgtc
      181 gccgccacgt cgatagacag cggcacgtgg gtcgccgtgc cgctcgcgct ctgtgtcgcc
      241 acggccgcgg gagccttcaa cggcgcgctc atctccggct ttcgcatcca gccgatcatc
      301 gtcacgctgg cgcttttgat ttcggggcgc ggcgtggcgc aggtcatcaa cggcgggcgc
      361 ctgattcctt tcagcgaccc gacgttcgag tatctcggcc gcggctacgt ggggccggtt
      421 cccgtgcagg tgctcgtgat gttcgtcgtc gtcgccgcgg ccatcttcgt gatgcgggcg
      481 acggccttcg ggcgctacgt cgtggcggtg ggcggcaacg aatcggcggc gcgactctcc
      541 ggcgtgaagg tcaaccggac gaaggtgttg gtctacgcgc tgagcgggct tttggccggt
      601 ctcgccggac tcatcgagac ggcgcggcag ggggcgagcg acgcgccgaa cgtcggcctc
      661 aacatcgagc ttgacgccat cgccgccacg gtcgtcggcg gcacggccct cactggcggg
      721 cgcgccacgg tcgtcggcac gctcatcggc gcgctcatca tgcaggtcat caccacgagc
      781 atcaacatga agggctacga gtacgcctgg tcgctcgtca tcaaggccgc catcatcgtc
      841 gtcgccgtct acatccagcg gccgaaagtc gcctgacgcc ggggacgacg ccgaaaggaa
      901 atgagacgac cgacaggaag gctgccgaag caatgtctcg acacttccaa agcccgccgc
      961 ggccgacgcg aaagcgcctc atccgggcgc tcggttgcgt gtgcctctgg gcggtcgcgg
     1021 cggcggcgca gcaggcgggg ccgacctatg ccaacccggt catcgcgggc gacttccccg
     1081 acccgtcggt gattcgcgtc ggcgatgact actgggcgat gacgacgacg gggacgtggg
     1141 cgccgcactt ccccatcctc cactcgcgcg acctcgtcaa ctggcgcgtc gccggctacg
     1201 tcttccggcg caagcccgcg tgggttaaga gcgacttctg ggcgccggag atcacggaag
     1261 accgcgggcg cttcttcgcc ttctacacgg cgcggcgcga cgaggggccg aagcgcgtgg
     1321 ggacgctgtg cgtggccgtc gccaccgccc gccgtcccga ggggccgtac accgaccgcg
     1381 gcccgctcgt ctgcgaaatc cgcgagcttc ggaacgtcgg ctccatcgac gccttcttcg
     1441 tccgcgacga gcgagatgtt ccacacctcg tctggaaggc cgacggcaac gacgccgagc
     1501 cggacgtgcc gacctccgtc ttcgcgcagc gcctctccgc agacggaaca aagctcctcg
     1561 gccgtcgccg ggaaattcta cgcaacaccg aaggctggga gcgccacgtc accgaaggct
     1621 ccgacatcgt ccgccgcaat ggttggttct accacttcta ttcgggcaac gcctgctgcg
     1681 ggcgcggttg cgattacgcg ctcggcgtcg cgcgctcgcg ccgcctcctc ggcccgtggg
     1741 agaagagtcg gcgcaacccc atccttgacg acaactctga ctggcagtgc cccggccacg
     1801 gcaccatcgt caccacggcc gacggccgcg acttcctcct ctaccactcc taccggcgcc
     1861 gccgagacac cttcaacgtc gggcgcgagg ccctgctcga cgaggtgaag tggggcgccg
     1921 acggctggcc gacaatcaac gacggccgcg ggccgacgag cctcgccgcg tcgccccacg
     1981 ccgcgcccgt gtcggacgac gaaggggaag tcttcgacga cttcgcgggg ccgcagctcg
     2041 gactcgaatg gcagtggccg atgttcttcg agcagtcggc gcgcgtggag ccttcgcggg
     2101 gcggccgcct cgtcgtcgaa atcccgaagg gcgcgcgcgc agacgagtgg acggccgcgg
     2161 tcctggcccg caggacgacc tcgggcagtt acgtggcgac gacacaggtt gacctgcgcg
     2221 gcacgaccgc gggcgctcgc gcggggcttt cggcctacag ttggcgtgag tggtcggtgg
     2281 gcgtcgccgc gggcggcgga aaaatcgcgg tgtggcggcg tgagggcaaa gacttgcgca
     2341 cgctggccac cgccgacgcg ccctccgcgc ccgccgtcca actgcggatg acggcgagcg
     2401 gcggcgagac ctaccgcttc gcctacagcg tggacgggcg cgagtggacg gacgtgggcg
     2461 gccccgtctc ggccggctac atc
//
LOCUS       DASVES010000416         4802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_657816, whole genome shotgun sequence.
ACCESSION   DASVES010000416 DASVES010000000
VERSION     DASVES010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657816"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VF240_17055"
     CDS             complement(<1..1250)
                     /locus_tag="VF240_17055"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX7176972.1"
                     /translation="MGEHHVETSSDDCKVRAFMRALLNDLGALERMLDEGRIESGVRR
                     IGAEQEMFLVDRHMRPAPVAGEVLGRVNDARLTTEIARFNLEANLTPRLLEGDALRRL
                     EGEVGEVIRLVRDGARQFGADVLLAGILPTLRRSDLDLSNITPSPRYYQLNDAIMRMR
                     GGAINAHIKGLEEVNVTSDNIMMLSSNTSFQVHLQVGPEEFVPLYNMAQAVTAPVLAA
                     AVNSPIWLGNRLWQETRLALFQHSADARNSSQLARQFPTRVGFGEGWLRRSVVELFRE
                     DIARFRVILTTEVDEDPAEVLARGGVPRLKALGLHNGTVWWWNRPCYGVSEGKAHLRI
                     ENRVIPSGPTVLDEVANAAFFTGLMQALPAVYGEIDKLISFDDAKANFLSAARHGLDA
                     QFTWVGGERQTAAGLILEHLLPLAR"
     gene            1425..2084
                     /locus_tag="VF240_17060"
     CDS             1425..2084
                     /locus_tag="VF240_17060"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7176973.1"
                     /translation="MTFDGQIAAGSATPGEYCAGVGHAPEEVIRLLGERRRREEQIVE
                     PCRLDLLPLTRLIGYIIDKHHVYARRQVAAIAALLAKLSAGHENSPPELARVRGLFKV
                     LRQELLFHMEKEEVSLFPQIIRTETNLCRPEPPAAPDFGPMRDPVRMLMHEHDQLCAM
                     LDEIRAATDDYTPPRGGGASYRALCQSLRDLEFNLLQLIHLEDSVLFPRALGAEPRRH
                     S"
     gene            2461..3534
                     /locus_tag="VF240_17065"
     CDS             2461..3534
                     /locus_tag="VF240_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176974.1"
                     /translation="MSASVSVEHRRPVLLGLAASALLTVLVVVGSRNLEHYDAALFGY
                     TVASVVAFGAVVYRYSLWLRRPATRVYWRRGWQLFRERRKLMANTVSAGRTIAGNLVE
                     QKFIARRGFTRWLMHALIMWGCIISALITFPLVFGWIHFKLEGERGYRTYLFGFPLNA
                     LDGRSVAAWVMFHALDFTAVMVILGCAIAIRRRLRDRGAIALQQYMLDFVPHLLLISV
                     SVTGLMLTVSSLWLAGYMYSFIALSHQALVIMTLFYLPFGKLFHVVQRPASIGVELYQ
                     ERAREMPQARCARCGAEFVAEMWLGDLKQVIDQLGFDYRMPDGRTLQDYCPRCKRVMR
                     GLSYASLPDRYEKVFEGGMKDEG"
     gene            3591..>4802
                     /locus_tag="VF240_17070"
     CDS             3591..>4802
                     /locus_tag="VF240_17070"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7176975.1"
                     /translation="MAKVGNLEKIVETFGPHLHDEPHGGWRADRIIDKVVPTHCPYCA
                     VQCGINLLVEKNHVVGFEPRYDFPVNEGRLCPKGVTAYLQVHHPDRLLYPLIKRNGMF
                     ERASWDEALDLVVSKLKGLQEKHGKDSIGVYSGSSLTTEKTYLMGKFARAGLGTRYVD
                     YNGRLCMVSAAAGNNKAFGIDRAANPWDDIPLAEVLLIAGANCAETFPVLNKFLWQQR
                     DNGGRWIIVDPRETPTARQGDIHLQLKPGTDVALANALLHVIVEEGLTDEAFIAARTN
                     NWEETKQVAARYTPLVASEITGVPSEKIVQAARLYGRAKTSMMMHARGIEHHTNGVNN
                     VLSYINLVLATGQIGRPGSGYGTITGQGNGQGGREHGQKADQLPGQRSIMNPEHRKHV
                     CEVWDLPESELP"
BASE COUNT          824 a         1602 c         1533 g          843 t
ORIGIN      
        1 cgcgcgagcg gcaggaggtg ttcgaggatg aggcccgcgg ccgtctgccg ctcgcccccg
       61 acccacgtga actgcgcgtc gaggccgtgg cgcgcggccg agaggaagtt cgctttcgcg
      121 tcgtcgaagg agatgagttt gtctatctcg ccgtacaccg cggggagcgc ctgcatcagc
      181 cctgtgaaga aggcggcgtt cgcgacctcg tccaggactg tcggccccga cgggatgacg
      241 cggttctcaa ttcgcaggtg cgccttgcct tcactcacgc cgtagcacgg gcggttccac
      301 caccagaccg tgccgttgtg taggccgagg gctttcaggc ggggcacgcc gccgcgcgcg
      361 agcacctcgg cggggtcttc gtcaacctcg gtcgtcagga tgacgcggaa gcgggcgatg
      421 tcttcgcgga agagttcgac gacggagcgc cgcagccacc cctcgccgaa gccgacgcgg
      481 gtcgggaact ggcgggcgag ctgcgacgag ttgcgcgcgt ctgccgagtg ctggaagagg
      541 gcgaggcgcg tctcctgcca caggcggttg ccgagccaga tgggggagtt gaccgccgcc
      601 gccaagacgg gcgcggtgac agcctgcgcc atgttgtaga gggggacaaa ctcttcgggg
      661 ccgacctgca agtgcacctg gaagctcgtg ttcgaggaga gcatcatgat gttgtcggac
      721 gtgacgttga cctcttcaag ccccttgatg tgggcgttga tcgcgccccc gcgcatccgc
      781 atgatcgcgt cgttcaactg gtagtagcgc gggctgggcg tgatgttgct caggtcgagg
      841 tcggagcgcc gaagcgtcgg cagaatcccg gcgagcagaa cgtcggcccc gaactggcgc
      901 gcgccgtcgc gcaccagccg gatgacctcg cccacttccc cctccagccg ccgcaacgcg
      961 tcgccttcga gcaggcgcgg cgtgaggttg gcttcgaggt tgaagcgcgc gatctcggtc
     1021 gtcagacgcg catcattcac acgccccagc acttcgccgg cgacgggcgc gggtcgcatg
     1081 tggcggtcga cgaggaacat ctcctgttcg gccccgattc ttctgacgcc gctctcgatg
     1141 cgcccctcgt cgagcatccg ctccagcgcc ccgaggtcgt tcaacagcgc ccgcatgaac
     1201 gcacgcacct tgcaatcgtc actactcgtc tcgacgtgat gttcgcccat aatttttttc
     1261 cgtggacagc ccgacagggt aagcagggcc gcggtctgat tactgagttg cgcccggcgg
     1321 cgccttaacc cctaggtggt gctctggaaa gcaaattgca taccctcgtc cgtgagtcgc
     1381 ggcggggcgg gcgcgtttga atcgcattcg aggggggacg catcatgaca ttcgacggcc
     1441 agattgcggc ggggtcggcc acgccgggcg agtactgcgc gggagtagga catgcgccgg
     1501 aggaggtcat caggcttctg ggcgagcgac ggcgacggga ggagcaaatc gtcgagccgt
     1561 gtcggctaga cctgctcccg ctcacacggc ttatcggcta catcatcgac aaacatcacg
     1621 tctacgcgcg ccggcaggtg gccgccatcg ccgcgctcct cgccaagctc tccgcggggc
     1681 acgaaaatag cccgccggag ttggcccgcg tgcgcggcct cttcaaggtt ttgcggcagg
     1741 agttgctttt ccacatggag aaggaggaag tctcactgtt cccgcagatc atccgcacgg
     1801 agacgaacct gtgccggccg gagccgccgg cggcccccga cttcggcccg atgcgcgacc
     1861 ccgtcaggat gctgatgcac gagcacgacc agttgtgcgc gatgctcgac gaaatccgcg
     1921 cggcgacgga cgactacacg ccgccacgcg gcggcggcgc cagttaccgg gcgctctgcc
     1981 agtccttgag agacctcgag ttcaacctgc tccaactcat acaccttgaa gacagcgtgc
     2041 tcttcccgcg cgccctcggc gcggagccgc gccggcactc gtgaaggggg agattcggga
     2101 gcgcccgcgc cgaccgggag ggcgcaaaga atatttttag ccttcgccgc gcgcccctcg
     2161 tgtctgtgcg gggtggtcgc cccgaatgga aatttcccca acgccgaagg ggcggctgcg
     2221 ggagtttccc caacgcacac gcttcatcct cggtgcggcg gcgcgtgatt ccacagttta
     2281 gtgatattta tttttcaccg ttgcggttat catcaaactc cgagaaccac tgaaatgatt
     2341 tgtacacatg acgccggcgc tcccagtctc aggcggccgc gttgagggtg ggggcgctga
     2401 cgcgctcact catgcgtttc ggacgcgtcc ccggcttgca gactctttca aggaaagctc
     2461 atgtcagctt ccgtatccgt cgaacaccgc cgccccgtgc tgctcggcct cgcggcctcg
     2521 gcgctgctga ccgtgctggt cgtcgtcggc tcgcgcaacc tcgaacacta cgacgcggcg
     2581 ctcttcggct acacggtggc gtcggtcgtc gccttcggcg cggtcgtcta ccgctactca
     2641 ctttggctgc gccggcccgc cacgcgcgtc tactggcgcc gcggctggca gctcttccgc
     2701 gagcgccgga agctgatggc taacacggtg agcgcaggca gaaccatcgc cggcaacctc
     2761 gtcgaacaaa aattcatcgc ccggcgcggc ttcacgcgct ggctgatgca cgcgctcatc
     2821 atgtggggct gcatcatctc ggcgctgatt acgttcccgc tcgtcttcgg ctggattcac
     2881 ttcaagctcg aaggcgagcg cggctaccgc acatacctct tcggcttccc gctcaacgcg
     2941 ctcgacggac gcagcgtcgc cgcgtgggtg atgttccacg cgctcgactt cacggccgtc
     3001 atggtcatct tggggtgcgc catcgccatc cgtcgccgcc tgcgcgaccg cggcgccatc
     3061 gccttgcagc agtacatgct cgacttcgtg ccgcacctcc tgctcatctc ggtctccgtg
     3121 acggggctga tgctcaccgt gtcgagcctc tggctggcgg gctacatgta ttccttcatc
     3181 gcgctgtcgc accaggcgct cgtcatcatg acgctcttct acctcccgtt cgggaagctc
     3241 tttcacgtcg tccagcgccc ggcgtcaatc ggcgtcgagc tttaccagga gcgggcgcgc
     3301 gagatgccgc aggcccggtg cgcgcgctgc ggcgcggagt tcgtcgcgga gatgtggctc
     3361 ggcgacctga agcaggtcat cgaccagctc ggcttcgact accggatgcc cgacgggcgg
     3421 acgctgcaag actactgccc gcgctgcaag cgcgtgatgc gcggactcag ttacgcctcg
     3481 ctgcccgacc gctacgagaa agtctttgag ggagggatga aggatgaagg atgacggatg
     3541 aataagacgg ggtgaagctt cctcttcatc cttcatccct ctattttctt atggctaaag
     3601 tcggaaactt agaaaaaatc gtcgagacct tcgggccgca cctccacgac gagccgcacg
     3661 gcggctggcg ggccgaccgc atcatcgaca aggtcgtgcc cacccattgc ccgtactgcg
     3721 ccgtccagtg cggcatcaac ttgctcgtcg agaaaaatca cgtagtaggt tttgagccgc
     3781 gctacgactt ccccgtcaac gagggccgcc tctgcccgaa gggcgtcacg gcctacctgc
     3841 aagtccacca cccggacagg ttgctttacc cgctcattaa acgtaacggc atgttcgagc
     3901 gcgcgtcgtg ggacgaggcg ctcgacctcg tcgtctcgaa gctcaagggg ctgcaagaga
     3961 agcacggcaa agattcgata ggcgtttact ccggctcatc gctgacgacg gagaagacgt
     4021 acctgatggg caagttcgcg cgcgcgggct tgggcacgcg ctacgtggac tacaacgggc
     4081 ggctctgcat ggtctcggcc gccgccggga acaacaaggc gttcggcatc gaccgcgccg
     4141 ccaacccgtg ggacgacatc ccgctcgccg aagtcctcct gatcgccggc gccaactgcg
     4201 ccgagacctt ccccgtcctc aacaagttcc tctggcagca gcgcgacaac ggcggccgct
     4261 ggatcatcgt cgatccgcgc gagacgccga ccgctcggca gggcgacata cacctccagc
     4321 tcaagcccgg caccgacgtg gcgctcgcca acgctctgct ccacgtcatc gtcgaggaag
     4381 gcttgactga tgaggctttc atcgccgcgc ggacaaacaa ctgggaagag acgaaacagg
     4441 tggccgcgcg ctacacgccg ctggtcgcgt cggaaatcac gggcgtgccc tcggagaaga
     4501 tcgtgcaggc cgcgcgcctc tacgggcgcg ccaagacttc gatgatgatg cacgcgcgcg
     4561 gcatcgagca ccacacgaac ggcgtcaaca acgtcctctc ctacatcaac ctcgtactcg
     4621 ctaccggcca gatcggccgc cccggcagcg gctacggcac catcaccgga cagggcaacg
     4681 ggcagggcgg gcgcgagcac gggcagaagg ccgaccaatt gccggggcaa cgctccatta
     4741 tgaatcccga acaccgcaag catgtctgcg aggtctggga cttgcccgaa agtgagttgc
     4801 cg
//
LOCUS       DASVES010000417         7738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_271293, whole genome shotgun sequence.
ACCESSION   DASVES010000417 DASVES010000000
VERSION     DASVES010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271293"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1514)
                     /locus_tag="VF240_17075"
     CDS             complement(36..1514)
                     /locus_tag="VF240_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176976.1"
                     /translation="MIFIIGIVSISMLPVFAQNIASLDGPISGERGHGSNHPASLEKE
                     KKLNGLKKRMRALQAYILSVDPSSKDHQAAARSIDEIQSQIRELEANLSGSGAPHITI
                     NNSDARSVLTFFGGSAASKDYVKSGDTCVSEECFQFKRKVDSLPLGWPGRFARGLQYQ
                     YQRNEQTRSVLLSADVIIDNPQRYLSQHTLTLKFTELFPGRLPTYKRGNDYLKNYPGV
                     TSDTLNNLLCGDRPLITCLTKSGNWFERALMGTSVYVTLSERATIQQGTIVTSPRFGT
                     PYQWNGGVTFDPAKLFPTATDWKGTFVEVQKIDGALAYLGAADARSGRRRPWETPWPA
                     AFIPKVEFKRLSRFDFMKFQDVLIAAPFPSRALNTWTFTWDLTRVIPDTNSRFDADAI
                     AEALLKLEKGFKHKEQPTPTLQKQCILHFTNEDTPTDVQSSSSAETCQAIAKAVKAES
                     YALACESREGSGEQMRVIDRASGPLTRLVESPVKPARNFCKW"
     gene            complement(2024..2095)
                     /locus_tag="VF240_17080"
     tRNA            complement(2024..2095)
                     /locus_tag="VF240_17080"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2061..2063),aa:Cys,seq:gca)
     gene            complement(2184..2255)
                     /locus_tag="VF240_17085"
     tRNA            complement(2184..2255)
                     /locus_tag="VF240_17085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2221..2223),aa:Gly,seq:gcc)
     gene            2467..5178
                     /locus_tag="VF240_17090"
     CDS             2467..5178
                     /locus_tag="VF240_17090"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7176977.1"
                     /translation="MAVTTKRTRLVLGGLFFVTMFVYAGGIAYQTFETRRSSTGWNAL
                     VRGGRVIVEEVRPGGPAAELRPDDVVVALDGRRVDGPASVRDLAAREPGHEYALSVER
                     EGRPMELSLLTTTPPSFANTYYTLLVVVIPLIFICTGLAVFALRPEDKQAVLLALMFG
                     TFIPSGAATLRGLPDWLADAMVVGAVVSSFFAAFFFHFFLIFPERSPLLRRFPRLEYA
                     LYVPQLLLVAPYVAYTAYVQSAAVGNLFAERERTGPAGVAVASLIVAYVVAGVASLAF
                     NYRHAGRQSRRKMRIVVAGCALGFLPSLLLTAIYFYGPTRIDETRFKWLSAAAGVAFL
                     AFPVSFAYAIVRHQVIPVRLMFRRGVRHVFVARGSVLLEVVTVGLALTLFLQYIFNYV
                     RPSSGLVIGVVSGVFSVLVWNLTTELHYRVIAPAIDRRFFRRAYNAQQILADLGQSLR
                     PLTDMRETAQFVAARLREALQPENVSVFLRDAATGDYVCAVSSHHIDAGRVTLDGCAA
                     DLVLPREAFAVRRLRESSQPLAVDFHDPQSWASMLLSADLELTVSRRRESETLRQINS
                     SLLVPVATKSELLAVISVGPRLGDLPYTREDRQVLTAVALQMSFAVENALLLKRKVEE
                     DRLRREVEMATEVQRRLFPERPPWLDSVELAGICLPARGVGGDYYDFILLDEERVGVA
                     VADVAGKGISAALLMSTVQASLRSRAEQVDDDSLTDLVASMNRLLNRSTNSASYASFF
                     YALFDGRTRLLTYVNAGHNPPLLVRARARAARAGSSPAVEARRLSVGGGAETGGVLIS
                     SGAGAQTSYLTTGGPVIGLFDEFVYEQETIKTESGDVIVAYTDGVTEALNPHGEEFSQ
                     ERLEALVEEFAHLPAEELMRRIIEDLRRWCLNTPQHDDVTLVVVKVK"
     gene            complement(5313..>7738)
                     /gene="priA"
                     /locus_tag="VF240_17095"
     CDS             complement(5313..>7738)
                     /gene="priA"
                     /locus_tag="VF240_17095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7176978.1"
                     /translation="FTYRLPLALREEARVGARVLAPLGRKMTTGYVVALHSELDPATE
                     LDDAELKEAEELLDAEPLITPEVLEITRWISEYYVAPWGEVLKAALPAGINATVEQVL
                     TITPEGRDELARLPPRRAVTTKAEILRLTAGEGETTLHALARDFGQARASKAARELER
                     AGWVTLSHRSRSAAARAKRRKAVRLLPPASHAENGAARPLTNAQQRVVETLIKGGGEM
                     YFAALLEAADASASAVQSLERRHMVEVFVSDVRRDPLAGAILPNADDLKLTDDQRNVL
                     GEIEGALGERKYAAFLLHGVTGSGKTEIYIRAMRSALRKGRTAMMLVPEIALTPVFSR
                     RLRAHFGDLVAIFHSSLTTGERFDEWGRIRRGEARVVIGTRSAVFAPVSDLGIVVVDE
                     EHESSYRQQESPYYNGRDTAVMRALKERAVVVLGSATPSLESFHNARRGKYRHLQLPT
                     RVADRPMAKAEIIDMRQVFRGRGKQEVFSPDLLAAIEETHERGHQSIILLNRRGYSVF
                     ILCRSCGERVQCPNCDVTLTYHRGERCLICHYCNMRARVPEACPACRGEFIHYVGEGT
                     EQIEEILQKRFPALRIARLDRDTTSRRSTYEQAILRFGAGELDMLVGTQMIAKGHDFH
                     NVTLVGVVSVDAGLALPDFRAAERTFQLITQVAGRAGRGELPGRVLIQTFHPHHYALR
                     HACAQDYDGFFEEEIHHRKSMSYPPFVALASLLIHGDELGRAQATATEVRAALDRANA
                     DRHVRVLGPAPAPLARLRGEHRIHILLKSRDRNRLRNVIELALADASAVPGCDTTCVN
                     VEIDPVNLM"
BASE COUNT         1357 a         2305 c         2544 g         1532 t
ORIGIN      
        1 cgttagcgtg tattaagacc gctttctatt gcgactcacc acttgcagaa gttacgagcg
       61 ggcttgaccg gcgattcaac gagacgtgtc aaaggcccgc tagcgcgatc aataacacgc
      121 atttgttcac ccgaaccttc cctcgactca cacgcaaggg cgtaactctc agccttaaca
      181 gccttagcga ttgcttgaca ggtctcagca ctggacgatg actgcacatc agtgggcgtg
      241 tcttcgttag taaagtgaag gatgcactgt ttctgcaatg tgggtgtagg ctgttctttg
      301 tgcttgaaac ccttttccaa tttgagcaac gcctccgcta tcgcatcggc atcaaatcga
      361 ctattggtgt cggggattac gcgcgtcaga tcccaggtga aggtccacgt gtttaaggcc
      421 cgactcggaa acggcgcggc gatcagcaca tcttgaaact tcatgaagtc gaaccgactt
      481 agccttttga actcgacttt aggaataaag gctgccggcc acggcgtctc ccacggccgg
      541 cgacgccccg acctggcgtc ggccgcgccg aggtaggcga gcgcgccgtc aattttttgc
      601 acttcaacga acgtgccttt ccagtccgtg gccgtaggga agagcttcgc cgggtcgaag
      661 gtgactccgc cgttccactg gtaaggcgtc ccgaacctgg gggaggttac gatggtcccc
      721 tgctgtatcg tcgcgcgctc cgacagcgtg acgtaaaccg aagtccccat aagtgcccgc
      781 tcaaaccaat tgccgctttt agtgaggcag gtaattaacg gcctgtcccc acacagtagg
      841 ttgtttagcg tgtcactcgt gaccccgggg taatttttaa ggtaatcatt ccccctctta
      901 taggtcggca ggcgacctgg gaatagctcg gtaaatttaa gggtgagggt gtgttgactt
      961 agataacgtt gcggattgtc tatgatgaca tcggcactta aaagcaccga cctcgtttgt
     1021 tcatttcgct ggtactggta ctggagtcca cgcgcgaagc gtccgggcca accgagcggt
     1081 agagaatcaa ccttacgttt aaattggaag cattcctcgc ttacgcaggt gtcgcccgac
     1141 ttcacatagt ctttcgaggc ggcgctcccg ccgaagaatg tgaggacact tcttgcgtct
     1201 gaattattta tcgtaatgtg tggcgcacca ctacccgaca ggttagcctc taattctctt
     1261 atctgcgact ggatttcgtc tatggagcgt gctgccgcct gatgatcctt cgatgaagga
     1321 tcgacagata ggatataggc ttgtaatgcc ctcattctct tctttagacc gtttaatttc
     1381 ttctccttct caagagatgc aggatggttg ctaccgtgcc cccgttcgcc acttatggga
     1441 ccatctaatg acgcaatatt ttgggcgaaa accggaagca tcgaaataga aacaattccg
     1501 atgatgaaga tcaaggtgcg agatagatct ctcatgatgg ttcccttatt tggggtgagt
     1561 aaagtgaaag tagccgaagt aagacggcca ccaaagttac acagcagagc ttttctagct
     1621 ggtttgaagt taagccgact cggtgaaggc accggcttgt aggtagacgg attacggaag
     1681 aaggcggtga gggtgaaaat ctcaccgaca attaagccgc atggtgacct atcagttagg
     1741 acggctcacc acgttcgatg gtaaagcggc cgcaacttac tccttaataa attagttgtc
     1801 aacacttttg ttcagcatcc tagtctcgca gcgccatgtt gggacgtggt gattatttcg
     1861 ggcaaaattt aggacataag gattattatc aggcccccag aacatgaacc ggctgcgact
     1921 ccatccatca agaatggaaa cgcggcgtag gacttggcag accgttggca gaccaaacaa
     1981 ctacgggcag cctataggct gcccgtagtt attagtatta aagaggcggc ggtcggaatc
     2041 gaaccgacga ataaaggttt tgcagacctc tgccttacca cttggctacg ccgccggact
     2101 aagggatgag ggcggagggt tgaaggatga ataggaggtt gtctcgcttc atccctcatc
     2161 cctccgccct catcccttac ggaagcggga gacgggattt gaacccgcga cttcgacctt
     2221 ggcaaggtcg cactctacca ctgagttact cccgcgtgag cggattaatt ctacgaatcc
     2281 gcgtcacatt gtcaagcgtc gtggtgtgtt aaggaaagag cggaaaggat tgatggggaa
     2341 ggggaagtga gttgtacgcg ggtggagact tcagccccgc cacacccccg ctctcccccg
     2401 tctgcgccat gtttcttcgg aggtaactgt ggcatgatgg gtgcgaaagt tgccgagggc
     2461 tgaagcatgg cagtgacgac caaacggacg cggctggtgc tgggtggcct cttcttcgtg
     2521 acgatgttcg tctatgcggg cggtatcgct taccagactt tcgaaacgcg gaggagctcg
     2581 acgggctgga acgcgttggt gcggggcggg cgtgttatcg tcgaggaggt caggcccggc
     2641 gggccagccg cagagttgcg gccggacgac gtggtcgtcg ccctcgatgg ccggcgggtc
     2701 gatggcccgg cgtccgtccg ggaccttgcg gcgcgcgagc cgggtcacga atacgccctg
     2761 tcagttgagc gcgaggggcg gcccatggaa ctctcgctac tcacgacgac gcccccgtcc
     2821 ttcgccaaca cctactacac gctgctcgtc gtcgtcatcc cactcatctt catctgcacg
     2881 gggctggcag tcttcgcact caggccggag gacaagcagg ccgtgctgct ggcgctgatg
     2941 ttcgggactt tcatcccgag cggcgccgcc acgctgcggg ggctgcccga ctggctcgcc
     3001 gacgcgatgg tcgtgggggc tgtcgtctca agtttcttcg cggccttctt cttccacttc
     3061 ttcctcatct tccccgagcg ttcgccgctg ctccggcgct tcccgcgact ggagtacgcg
     3121 ctctatgtcc cacaactcct gctggtggcg ccttacgtag cctacacggc atacgtgcag
     3181 tcagcggcgg tcggcaatct gttcgcggag cgggagcgaa ctggccccgc gggtgtggcc
     3241 gtcgcctcgc tgatcgtcgc ctacgttgtg gccggggtgg cgtcgcttgc gttcaactac
     3301 agacatgcgg gcagacagtc gcggcgcaag atgagaatcg tcgtggccgg gtgcgcgctc
     3361 ggctttctgc cgtcgctgtt gctaacggcc atctacttct acgggccgac gcgcatcgat
     3421 gagactcgct tcaagtggtt gagcgcggcg gcgggcgtcg cctttctggc cttccccgtc
     3481 tcgttcgcct acgccatcgt ccgccatcag gtcatccccg tccgcctcat gttccgacgc
     3541 ggcgtccgac acgtcttcgt cgcgcgcggc tcggtgctcc tcgaagtggt cacggtcgga
     3601 ctggcgctga cactcttcct gcaatacatc ttcaactacg tgcggccttc gagcgggctg
     3661 gtgatcggtg tggtctcggg cgtgttctcg gtgctcgtgt ggaacctgac cacggagctg
     3721 cactaccgcg tcatcgcgcc ggccatcgac cggcgcttct ttcgtcgcgc ctacaacgcg
     3781 cagcagattc tcgccgacct cggacagtcg ctgcgcccgc tcacagacat gcgcgagacg
     3841 gcgcagttcg tcgccgcgcg tctgcgcgaa gctctccagc cggagaacgt gagcgtcttt
     3901 ttgcgcgacg ccgcgacggg cgattacgtg tgcgccgtct cgtcgcacca catcgacgcc
     3961 gggcgcgtga cgttagacgg ctgcgccgcc gacctcgtgc tcccgcgcga ggcgtttgcg
     4021 gtgcggcgtc tgagggagtc gtcgcaaccc ctcgctgtag atttccatga cccgcagtcg
     4081 tgggccagca tgctgctctc agccgatttg gagctgacag tttcgcgccg gcgcgagagt
     4141 gagacgctgc gacagatcaa ctcgtcgctg ctcgtccccg tggcgacgaa gagtgagctg
     4201 ctcgccgtca tctcggtcgg cccgcgtctc ggcgacttac cctacacacg cgaggacagg
     4261 caggtgctca cggccgtcgc gttgcagatg tccttcgccg tggagaacgc gctgctgctc
     4321 aagcgcaagg tggaggagga caggctgcgg cgcgaggtgg agatggcgac ggaagtgcag
     4381 cggcgtctct tcccggagcg ccccccgtgg ctcgactccg tggagctggc gggcatctgt
     4441 ctgccggcgc gcggcgtcgg cggcgactat tacgacttca tactgttaga cgaggagcgc
     4501 gtcggcgtgg ccgtcgcgga cgtggcgggg aagggaatct cggcggcgct tttgatgtcc
     4561 accgttcagg cgtcgctccg cagccgcgcc gagcaggtgg acgacgacag cctgacagac
     4621 ctcgtcgcgt cgatgaaccg cctgctcaac cgctcgacca actcggccag ctacgcctcc
     4681 ttcttctacg cgctctttga cggccggacg cggctgctga cttacgtcaa cgccggccac
     4741 aacccgccgc ttctagttcg cgcgcgcgcc cgcgccgcgc gggccgggtc gtcgcccgcg
     4801 gtcgaggcgc gtcgcctgtc ggtcggcggc ggcgcggaga cgggcggcgt gttgatttcg
     4861 agcggggcgg gcgcgcagac gagttatctg acgacggggg ggccggtcat cggcctgttc
     4921 gacgagttcg tctacgagca ggagacgatc aagaccgaga gcggcgacgt gatcgtcgct
     4981 tacacggacg gcgtcacgga ggctttgaac ccgcacggcg aagagttcag tcaggaacgc
     5041 cttgaggcgc tcgtcgaaga attcgcgcac ctgccggccg aggagttgat gaggcgcatc
     5101 atcgaagacc tgcgacgctg gtgcctgaac acgccgcagc atgatgacgt gacgctcgtg
     5161 gtcgtgaaag taaagtaaaa aaacagtacc gcgagcggta gcgagcgggt gctcctgtga
     5221 tgagagtcct gactcctgca cgcgagaccc gctcgctacc gctcgcggta ctgttatcac
     5281 accaagcccc gccgtcgcgc gcggtactgt ttctacatca agttcaccgg atcaatctcg
     5341 acattcacac aggtcgtgtc gcagcccggc acggcggagg catcggcgag cgccaactcg
     5401 atgacgttgc ggaggcgatt gcggtcgcgc gacttgagca ggatgtggat gcgatgctcg
     5461 ccgcgcagac gcgcgagcgg ggccggggcg gggccgagga cgcggacgtg tcggtcggcg
     5521 ttggcgcggt cgagcgcggc gcggacttcc gtggccgtcg cctgtgcgcg cccgagttcg
     5581 tcgccgtgga tgagcagtga ggccagcgcg acgaagggcg ggtagctcat gctcttgcgg
     5641 tgatgtatct cttcctcgaa gaagccgtcg tagtcctgcg cgcaggcgtg acgcaacgcg
     5701 tagtggtgcg ggtggaaggt ctggatgagg acgcgccccg gtagctcgcc gcgtccggcg
     5761 cgtccggcca cctgcgtgat gagctggaag gtgcgctcgg cggcgcggaa atcgggaagc
     5821 gccagaccgg catcgacgga gacgacgccg accagagtca cgttgtggaa gtcgtggccc
     5881 ttggcgatca tctgcgtgcc gacgagcatg tcgagttcgc cggcgccgaa gcggaggatg
     5941 gcctgctcgt aggtcgagcg gcgcgaggtg gtgtcgcggt cgaggcgggc gatgcggagg
     6001 gcggggaagc gcttctgtaa aatctcttcg atctgctcgg tgccctcgcc gacgtagtgg
     6061 atgaactcgc cgcggcaggc cgggcacgcc tcggggacgc gcgcgcgcat gttgcagtag
     6121 tggcagatga gacagcgttc gccgcgatga taggtgagcg tcacgtcgca gttcgggcac
     6181 tgcacgcgct cgccgcagga gcggcagagg atgaagacgg agtagccgcg ccggttgagg
     6241 aggatgatcg actggtggcc gcgctcgtgc gtctcctcaa tggcggcgag caaatcgggc
     6301 gagaagactt cctgcttgcc gcgtccgcga aagacctgac gcatgtcgat gatctcggct
     6361 ttggccatcg ggcggtcggc gacacgcgtc gggagttgga ggtgtcggta cttgccgcgg
     6421 cgcgcgttgt ggaatgattc gagtgagggc gtggccgagc cgaggacgac gacggcgcgc
     6481 tccttcaagg cgcgcatgac ggccgtgtcg cgcccgttgt agtagggcga ctcctgctgc
     6541 cggtaagacg actcgtgctc ctcgtccacg acgacgatgc cgagatcgga gacgggagcg
     6601 aagacggccg agcgcgtgcc gatgacgacg cgcgcctcgc cccggcggat gcggccccac
     6661 tcgtcgaaac gctcgcccgt ggtgagcgac gaatggaaga tggcgacgag gtcgccaaag
     6721 tgcgcgcgca ggcggcgcga gaagacgggg gtgagcgcga tctcggggac gagcatcatc
     6781 gccgtgcgcc ctttgcggag cgcggagcgc atcgcgcgga tgtagatttc cgtcttcccg
     6841 ctccccgtca cgccgtgcag gaggaaggcg gcgtacttgc gttcgccgag cgcgccttcg
     6901 atctcgccga gcacgttgcg ctggtcatcg gttagcttga ggtcgtcggc gtttggaagt
     6961 attgcgcccg ccagcgggtc gcggcgcacg tcgctgacga agacttcgac catgtgccgc
     7021 cgttcgagcg actgaacggc cgaggcgctc gcgtccgccg cttccaggag cgccgcgaag
     7081 tacatctccc cgccgccctt gattaaagtt tcgacgacgc gttgctgcgc gttcgtgagc
     7141 ggacgcgcgg cgccgttctc cgcgtgcgac gcaggcggca gcagacggac ggccttgcgt
     7201 cgcttggcac gcgcggcggc gctgcgcgag cgatgcgaga gcgtgaccca accggcgcgc
     7261 tcaagctccc gcgccgcctt cgaggcgcgc gcctgcccga agtcgcgcgc gagcgcatgg
     7321 agcgtcgtct cgccctcccc cgcggtgagg cggaggattt cagccttcgt ggtgacggcg
     7381 cggcgtgggg ggaggcgcgc gagttcgtcg cggccctccg gcgtgatggt caaaacctgt
     7441 tcgacggtgg cgttgatgcc ggccgggagc gcggccttca acacctcgcc ccacggggcg
     7501 acgtagtact cacttatcca tcgcgtgatc tccagaactt ccggcgtgat gagcggctcg
     7561 gcgtcgagca gttcctcagc ttctttgagt tcggcgtcgt cgagttccgt ggcggggtca
     7621 agctcggaat gtaaggcaac gacgtagccg gtcgtcatct tccgtccgag cggggcgagc
     7681 acgcgcgcgc cgactcgcgc ctcctcgcgg agcgcgagcg gcagacgata ggtgaagg
//
LOCUS       DASVES010000418        30709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_76501, whole genome shotgun sequence.
ACCESSION   DASVES010000418 DASVES010000000
VERSION     DASVES010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 30709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30709
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76501"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VF240_17100"
     CDS             complement(<1..1242)
                     /locus_tag="VF240_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7176979.1"
                     /translation="MPASREGLRATAMFCLIATVLAAAVLATASAQSGGFSPQSPAKE
                     YIYVGGRLVATEEPSAMSPAAPTNAGAAGPSGLSWTDNSNNETGFKIESCKLVNSACT
                     QWQQIATVGANVVKYREGDLLGCEYKYRVRATNGVGDSAYSNEARPKPPCLIEGGGSL
                     SEEAVIWANVSGATASGNNLSKSIPSYAWDAGAVSTRAIASGDGYVQITPDHTGTYRM
                     FGLSKGDSDQNYTDIDYCLGLGEGILVVYEAGVSRGYVGTYAAGDKLAVVVEAGGVKY
                     YRNDTLLYTSTVSLSPASYPLRLDTSLSTSYSTLTDAKIAAVSFGGLQPPEAVTWKNV
                     TSTVQAVGNTLRKVSGTNTWYDAGASSIKGIDPGDGYMEFTPGEASTWRMVGLGNGDT
                     SVHYADIEYAFYMVGGGGLHIY"
     gene            complement(2454..3278)
                     /locus_tag="VF240_17105"
     CDS             complement(2454..3278)
                     /locus_tag="VF240_17105"
                     /inference="COORDINATES: protein motif:HMM:NF033520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEX7176980.1"
                     /translation="MNDELIITSYVIIDDVMRSLNHSSHPLAKVTDAEVLLVAVVAAK
                     YFRNNHERALWVLTELGYLSERLSTSRLNRRLHAVADWLALTLEALGELFASGAAFII
                     DSMPLPVCLRARARRNKKVRGRDYCGYCAAKREKFFGWRLHLVCTPEGLPVSFAIVAG
                     GYHDLTPLHELTFLLPAGACVFGDKAYNSAPDEAAILAETGVRVIPIRRKNMAQHEWA
                     DEFDLKRYRKGIETLNSQLEKMGAQQLHARTNAGFEVKVHASLFALACSNINQQSR"
     gene            6370..9141
                     /locus_tag="VF240_17110"
     CDS             6370..9141
                     /locus_tag="VF240_17110"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX7176981.1"
                     /translation="MPRPRLLRPYAARLLLFTFVLTLAVWSALPPGYAAKALPTAPAA
                     SNYVVTTTGDAPDSNTADGVCNDGAGNCTLRAAIEQANVDDTSDMITFAPGVTGVISL
                     LTPLPDLTSMTITGPGPSQLTVRREDAAPSTFRVITLLPFRSLNISGITISGGYSGRG
                     GGIRVEGSPVSLTNVVVSGNTADGGISFGSEGRGAGIYQSAGVLNLINSTVSGNVTKN
                     AANNSGHHGAGIYTTSNLNVTGSTIAGNRIENGGGAGGVGLGGGIYVASGSVALTNST
                     VSGNSAGATGGQGGGIFKQGGTVMAKSCTVTENTADSAGGGIANSAGTFTLQNCIVAN
                     NAANSAPDLFGAFSSQGYNLVRDVTGATITGATAHNITNLDPLLGPLQNNKGPTQTHA
                     LLSGSPAVDAGDSGGLTTDQRGLLRPIDLSAVNVSDGADIGAYEVQAGQIQFGSATYS
                     VAEGAGFATLSVTRTGGDSVATSATFATSDGSATAGADYVGATGTITFAADEVAKDIT
                     VPITDDSLIESDETFTVTLSAPTGGATLGTRSAAAVIIVSDDVGPALPFVQFSASEAS
                     VGEAAGALNVSVQRTGDLSGTSSVSYATSNGTANDRSDYTAAYGMLIFTPGQESKTIT
                     VFVTDDRYQEPNETFTITLSGASGAALGLNQSVTATIASDDSASGLSPVKGGPSFDPE
                     FFVRQHYLDFLNRAPDAAGLAFWSNQTTNCGDADLLVCRINVSAAFFLSGEFQETGFY
                     TIRAQRVAYGKKSTEEATRVTFAQFIRDARQVGEGFVDGQPGAGAVLENNKNAYALQV
                     AGEAAFAQRFPQTTADAYVDALFASAGVTATAQERQNAVNAYGGGGAAGRAATLRSVA
                     DSASVRNAEFRSAFVLMEYFGYMRRDPDQAGYTFWLGKLNEFGGNYIAAEMVKAFITS
                     GEYINRFGQ"
     gene            complement(9640..10434)
                     /locus_tag="VF240_17115"
     CDS             complement(9640..10434)
                     /locus_tag="VF240_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176982.1"
                     /translation="MFTHPELLVSRLLFVGLIVEAFDIFKLRRAFGDDGMFSRSNIAI
                     LTSGARWQVRIGATAGGSAAMMLAMAAQGLSALVVIIKGTHTRAGMYSALICLVTNGY
                     VRSRRQIGGSGAEQLTFIVLVTFGLVLLAGGTDSARRLGDAFIAAQVVLAYFASGVAK
                     AASPIWRNGGAMTGILSTEGYGLPGLAKLLGRYPKLDWLLCWSVIGWEITFPLVLFVP
                     KPLMLVLLSVGVVFHVSCAIVMGLNRFVWAFCGCYPAVWATAMLLS"
     gene            complement(10418..10957)
                     /locus_tag="VF240_17120"
     CDS             complement(10418..10957)
                     /locus_tag="VF240_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176983.1"
                     /translation="MDIPLLTIAAIFAVWWAISVCNQFRSGAWTLRLRRHIPLGLIPL
                     WTFFAPNPARADSRLLWREEHGNEWGVWRELHFGFAPASSRWLVNPELIPNKAVTDLV
                     GSLLRVRPKREDRSALLCSPYLTLLSIVLAQFRRTGCSSIQFAIVRTSTDFPARRVEV
                     VFLSEVHDTADSVTHVYSS"
     gene            complement(10972..11298)
                     /locus_tag="VF240_17125"
     CDS             complement(10972..11298)
                     /locus_tag="VF240_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176984.1"
                     /translation="MQDKQGKKSSPHKPIKKTSLSHMELAAIDLTITALQAAGHKIND
                     RIAKDDNPREQWATAWADAHHPLTELTERDREIIARIKELAGQLSSRTSLKELLNARG
                     KIVQGG"
     gene            complement(11641..12210)
                     /locus_tag="VF240_17130"
     CDS             complement(11641..12210)
                     /locus_tag="VF240_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176985.1"
                     /translation="MVKAKRSDVVKATATLRERFISTLSAANKRAPAAGALEAFRQTL
                     KECEGEKAAPWRDVTDPLDAALAVLLTTDASTLTTDAIAELWTLQAREFKDDLGIKTA
                     PPLEKALIQHAAVCWVRLSILEMRYTNVMKQSITLTLGAYWEKRLTAAQKRFTRACES
                     LERVRKLSARVPKVQINVAAEGGRQVNVA"
     gene            complement(12215..12787)
                     /locus_tag="VF240_17135"
     CDS             complement(12215..12787)
                     /locus_tag="VF240_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176986.1"
                     /translation="MPKAKRVVSLAEREKEKWEREAGLYAEAMMKDSPSAEARAYYDA
                     QLDADPEEWRRHGDLTAEALDLAFEKFWLGYVTKESVRRGAELLKQELGYAEALPAER
                     ILIEHAVLCHVRLGMIEHLYSRNTSGSYTVSLAEHYEKRLTLAQRRFTRSVVTLARVR
                     GLLARADAARESARRASSARSLAVLKQMAG"
     gene            complement(12774..13085)
                     /locus_tag="VF240_17140"
     CDS             complement(12774..13085)
                     /locus_tag="VF240_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176987.1"
                     /translation="MPWEERSGRRYYYRKVRKGTRVFSVYEGGGLTGALAEARDKDER
                     RERVRSREEVRRELAHADRVEALVERSWLVAETAAKSVLESAGYHLHHRQWRLKRNAE
                     G"
     gene            13572..14249
                     /locus_tag="VF240_17145"
     CDS             13572..14249
                     /locus_tag="VF240_17145"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX7176988.1"
                     /translation="MCGRFTQRADRKALLKSFAVAEVPHLEPRYNIAPTQDVLGVHQS
                     PDGREATFFKWGLVPSWAKDTSMSARLINARSETVAEKPAFRQAFKQRRCIIPADGFY
                     EGQRTEGRKQPFFFRMRDERSFGFAGLWEHWEGEGGKVINSFTILTTEANEVLRPVHD
                     RMPVILHPEDYELWLGAGARELDLVKEMLRPYTSGEMVGYPVGVAVNSPRNQGAGLIE
                     RAAVNSA"
     gene            14273..14683
                     /locus_tag="VF240_17150"
     CDS             14273..14683
                     /locus_tag="VF240_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7176989.1"
                     /translation="MTYALWIVQALLALLFLFAGGTKLVIPPDVLASMGSPNQIPLPG
                     WFVRFIGVAEVLGALGLLLPGLLRVKPWLTPLAAAGLVIIMIGATALTMAADGVAAGV
                     VPLVVGLLAAFVAYGRWRLAPHAARREMIRRRSS"
     gene            14714..15160
                     /locus_tag="VF240_17155"
     CDS             14714..15160
                     /locus_tag="VF240_17155"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7176990.1"
                     /translation="MTTENSRAADEARIRELIEERVRAIRAKDVDALMSNHAPDVVMF
                     DALDPLRYVGSEAVRERAGQWLSWYQGPVGYEVRDLSVTAGGEAAFCHYLYRVSGTMT
                     NGSEVNMWVRSTVCLRNTEGEWVVTHEHTSVPFDAESGKASVDLKP"
     gene            15335..15931
                     /locus_tag="VF240_17160"
     CDS             15335..15931
                     /locus_tag="VF240_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7176991.1"
                     /translation="MSKVVAIMSMSLDGYVADLNDGVAEVFDWYMNSGDVEFHTGGSD
                     PMTFKVSGPSAEHLRGLWSELGAVLTGRRTFDVAHGWGGNHAWGPAFVLTDHIPAGWP
                     RPDSTVHFVTDGIESAVNQAKAAAAGKSVGVHGADTIQQFLNAGLIDELSIDIAAVLL
                     GSGVRLFDHLAGTPAVLGNPTVIPGVGVTHLRYPVRKA"
     gene            16126..16611
                     /locus_tag="VF240_17165"
     CDS             16126..16611
                     /locus_tag="VF240_17165"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7176992.1"
                     /translation="MVKPAKNTICLWYDGDAEDAARFYAETFPDSSVGAVHRAPGDFP
                     SGKQGDVLTVEFTVMGIPCLGLNGGPEFKHNEAFSFQVATTDQAETDRYWNAIIGNGG
                     QESACGWCKDKWGLSWQITPIALTEAITNPDRAAAKRAFDAMMQMKKIDIAAIEAARR
                     G"
     gene            complement(16980..17613)
                     /locus_tag="VF240_17170"
                     /pseudo
     CDS             complement(16980..17613)
                     /locus_tag="VF240_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            18336..20102
                     /locus_tag="VF240_17175"
     CDS             18336..20102
                     /locus_tag="VF240_17175"
                     /inference="COORDINATES: protein motif:HMM:NF036842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176993.1"
                     /translation="MQDSILFWNAVALEANRVSHSDPDKRQQNGPTLSSRAIAIVHLA
                     MYDAYAGVVGGAFARYLPAPPPPPPPGSSARDAVAGAAHRTLSRLYSAQTEFFDAQLS
                     CFNIADPSFAFGEAVGQALLDLRANDMDARSCGYMPSNDRGRHRVDPDNPGQGFDSPF
                     YGAQTRTFAVRRRHRSDPPPFGDGGVTKYLQALRDVRARGIRPDLTGTLPNPLFNRRR
                     TSEQTLTGIYWGYDGANRLGTPPRLYNQIVRQVAVERGNTEAQNARLFAYLNAALGDA
                     GILCWEQKYCHDFWRPVVGIREHDETLGPEAPHGTITGVNFNDADPSWLPLGAPSTNS
                     PGMKNFTPNFPAYPSGHATFGAAALHITRMFYGIAPNNKSGDPLFPNEFVSDEFNGGS
                     RDNDGTVRPRHTRFFPGGLWDMIIENALSRIFLGVHWIFDAFNFTASGGNLTPSLTDE
                     DIGGVGLGLRIARDIFAHGGGSAGAPQMTPAGVNPPIITPDPGPPAPPLPVHPAQPTA
                     QRNCVAARAAGSKKSGKRGAKKSSKKGATKGAAKKAGKGAAPPEMVGQQQPAWPSGLS
                     EQSEDQPGNTGPGAWPSGISEK"
     gene            20607..22931
                     /locus_tag="VF240_17180"
     CDS             20607..22931
                     /locus_tag="VF240_17180"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX7176994.1"
                     /translation="MKKIVCLSVFVISLVSMLQHPNVLSQGNPQSPEQIGKPCGADPA
                     CDPNSPKQGTFKAPINTDGKPKGWVVKFYHKKHHQKRESWVDEAHGRGLTHIKKHAKE
                     FDLVDPHAELELLSVERDDLGITNVLFEQVYKGVPVYGGKLAIRFDANGELLTYPAEE
                     GERRGIDGQIQKGKERKGIDGKIYATAKHVDTKPRLDSAQAITAAKSALSYTGPFSKS
                     PEAELFILPRQVGNSGPADLSGGATLVYKVELLIEDGVRTPERHWYFVDAHDGSVVWH
                     FDAMGQGAGQSWYSGYVWIPTGWFAWNNTGPHCGLTQWNNGFWLWDGSGYRGNMETLD
                     LKNTQPAYNFYYYSMMRFDGVDDRWGMPTEYGAWPAPDCNWQRQQAGVDTQYGMALAW
                     DYFLDTHGRRGIDNAGFRMFSRIHYGNNFDWAAWNGYNLTFGDGSSGRPWVSIDAVAH
                     EWTHGINEKTANLHLGGESGASAESFSDIFGTMIEFYASNWVNPPDWLVGEDFFPGGK
                     RDMYNPPSKGHPDHFNNRVYPGHCWPESANDNCGVHSNSGIQNKAFFLLAVGGTHPYS
                     GISVPGVGNDVAARVFYRALTSHLLAKPYARLYQVRNATYNAAVELYGYHSTVGDKVE
                     EAWRAVGVPSNPIYESWYFVEQHYKDFLNQSPDPGGWDYWANQINRNCHPNDQGCIEW
                     RRVMVSRAFWESGAFRERAEVKNSGLLNPPGSARPYDNRQFVRWCYKMYLNREPDQGG
                     WDFWTNDLNGHGDYNITINAFLRSGDYHYRYAFL"
     gene            22947..23597
                     /locus_tag="VF240_17185"
     CDS             22947..23597
                     /locus_tag="VF240_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176995.1"
                     /translation="MKWAGIHVTVKKEAIPVGPLILCGILAALSNCSPRQAKDRGVAQ
                     APGGALRGRNAVLELPPQARGLALPTEGDMWVVVVSTTAGFSGDGAGGVAITSQGGVA
                     AWHPSTEYRHPCRAELGGEAVRAVAQSTTSASPERWSASYVNPERPQGVADDLGTELV
                     LYLRKANGVEKHVSRWHESASHTRPKDLAALHEAALKIQSQVLGSCVEPPQGSKGG"
     gene            complement(23778..24752)
                     /locus_tag="VF240_17190"
     CDS             complement(23778..24752)
                     /locus_tag="VF240_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7176996.1"
                     /translation="MQLNQACTKFVSGYFSTNERTKKTKAAYRSDLTQFYVFAGEELL
                     LKSVSGALIERWCAHLHQRGYSPTSIRRKMVVLKVFCSYWVRKGSLRESPFWRVKLSY
                     GRVEQLPRALSAREMRKLLSQAKRNNSSTTAFLNNEGAARLDSFRASSPEYRSLRNLA
                     LVDLLFATGLRVGEVSSLDVQDFFMRESVFRVKGKGGRDRLAVVVDEETVRLQREYLE
                     VRTRIETRSPALFLNSSGQRLTTQGIANVIAQLREEAGIERHVTPHMLRHTVATLLLH
                     NGVDIRIVQEFLGHASIATTQRYTHVTKEHLRGVLRKRHPSLGMRTKK"
     gene            25119..26090
                     /locus_tag="VF240_17195"
     CDS             25119..26090
                     /locus_tag="VF240_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176997.1"
                     /translation="MTIAVVVAVARAAALVVAAYFDNSLWLPGEGVGLFEHPGVLAIV
                     VGDAVLFLLCVYASRMTRSVGRRLPTDRRPLVQRYFRLIILRSIFDRGGSFSKMLLFL
                     SVIGALSLVNQTIQLTDASAYYGHDTFDSILHRRSFWVMRVNLFISWCIIIPLLSSYL
                     IAHTKAIRLFFRRCDGHGLVKFQPGHPDQHGGFTFFGWLDTIYAIGLLTVLIEVFLLI
                     ITHRRVTAGNLFSLMGITVGGVLISVLSIYEVVRVVRRQERLLKMAAFVRTVRGSRQL
                     SQDYLALVYDAKFSPYSRTALRLAVALRAAALIPVAIRAMTYITSKL"
     gene            complement(26074..26619)
                     /locus_tag="VF240_17200"
     CDS             complement(26074..26619)
                     /locus_tag="VF240_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7176998.1"
                     /translation="MRAILNPGNIVSLVRVPLALLFVGLFRREPGSLLTASVVVAVIA
                     QFTDHLDGYLARRTTGKTVTGWLFDSVADRAFYIAAVIAFDREYGLGSFLVWSFTFRE
                     ICLYAFRVGVGDFDQLVPGFRKLVLIHAGVVRLAIVIGCLVPYGILPASLNNSTVVSA
                     ILAAATAFGYLCLYVLVKALR"
     gene            28145..28711
                     /locus_tag="VF240_17205"
     CDS             28145..28711
                     /locus_tag="VF240_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7176999.1"
                     /translation="MAARMGDILDRETSSHTSFASFVDHYLRLIDFPSDVIEVLENGD
                     INLFEAEQLARVTAERLGVTTSQAKRTRAALLASHLQTKASGEKLRQRVNELFHTSSA
                     EAGETSIESAELATELEDLEDFDPYDSTHLFWEQLKQLGFAFREIRREDVTDEEIEEL
                     LKASEPILAILGRIQRRREQTKIIKVKL"
     gene            complement(29025..30086)
                     /locus_tag="VF240_17210"
     CDS             complement(29025..30086)
                     /locus_tag="VF240_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177000.1"
                     /translation="MMTELKEKTAGVLRVPKPKHKLGLKVPTALRLPHDDLIKPTLPS
                     QTTQTTPASHTSLTRHSDSQPDTAFEISPRRDFTKVPNSVAREVIPAGEFKGKSSQLY
                     QCLYSMTRGAIVPKRAVRISRPRLMKLAGIGARVTFDSNITHLCTIGLLQVRQIAGEH
                     EGNEYTVLLPEERTSATLTTLTSQSSVSSHAHKVDGLDRLETSQTSQGVNIESIDSSV
                     SPKTSFKTSTERSDDDEAFAALREVERELTGRSAGANQWREVAEVLATELRIAAARTT
                     VSSVPAFLAEHLRRRLFKKDRRQLAAEEASAPAVSPLPAGVDVKKCPDCQGSGYYYPE
                     GFEKGVARCQHANLRKAEG"
     gene            complement(30091..>30709)
                     /locus_tag="VF240_17215"
     CDS             complement(30091..>30709)
                     /locus_tag="VF240_17215"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7177001.1"
                     /translation="SPAGTKLEPLPLSEVIVESPVESLDLVPADIRLARFEMQPDYLT
                     HRLRNQLKEHGQDYDLVFIDCPPQLGKLLTGALYSADFVLVPCSADAMGLQGLSDLAF
                     TIEQVRKNVNADLEMLGAVINLYKPQRNLSAESRAAIESATEMVGHVFDTNLHDYSKI
                     AEAPSQKLPAVLYAKTHRASDQLWSLTDEVLDRLQISRHKISAVK"
BASE COUNT         6312 a         9065 c         9103 g         6229 t
ORIGIN      
        1 gtagatgtgc aggccgcccc cgccgaccat gtagaaggcg tactcgatgt ccgcgtagtg
       61 gacgctcgtg tcgccgttgc cgaggccgac catccgccat gtgcttgcct cgcccggcgt
      121 gaactccatg tacccgtcgc ctggatcgat gcccttgatc gaggacgctc cggcgtcgta
      181 ccaggtgttg gtgcccgaca ccttccgcag ggtgttgccg accgcctgga cagtgctggt
      241 gacattcttc cacgtgacgg cctcgggcgg ctggaggccg ccgaaggaga cggcggcgat
      301 cttcgcgtcg gtcagggtcg agtaggaagt gctgagcgaa gtgtccaggc gcagcgggta
      361 ggaggcaggg ctgagcgaga ccgtgctggt gtagaggagt gtgtcgttcc ggtaatactt
      421 gacgcccccg gcctcgacga ccacggccag cttgtctcct gcggcgtaag tccccacata
      481 ccccctggag acgccggcct cgtagacaac gaggatgccc tccccgaggc cgaggcagta
      541 atcaatgtcc gtgtagttct gatccgagtc ccccttgctc agcccgaaca tcctgtaggt
      601 gccggtgtgg tccggcgtga tctggacgta gccgtcgccg gaggcgatgg cgcgggtcga
      661 gacggcgccg gcgtcccagg cgtaggaggg aatggacttg gagaggttgt tccccgacgc
      721 cgtggcgccc gagacgttcg cccagatgac ggcctcctcc gagagtgagc ctcctccttc
      781 aatcaaacaa ggaggcttgg gcctcgcctc gttggagtat gccgaatcac ctaccccgtt
      841 cgtggccctg acgcggtact tgtattcgca gcccagaagg tcaccttcgc gatacttaac
      901 tacgttcgcc ccgaccgtgg ctatctgctg ccactgggtg cacgcgctgt tgacgagttt
      961 gcaggattcg attttgaacc ctgtctcgtt gttcgagtta tcagtccatg acagcccaga
     1021 cggcccagcg gccccagcgt ttgtcggggc tgccggggac atggcggaag gctcttccgt
     1081 ggcgacgagc cggccgccga cgtagatgta ctctttggcc gggctctgcg gcgagaagcc
     1141 gcccgactgc gccgaggcgg tcgccagcac tgccgcggcg agcacggtcg cgatcaggca
     1201 gaacatggcg gtggcccgca gcccttcacg cgaggcaggc atagggagaa ggcgtcgtcc
     1261 tcccggctga tgcttcttac tcttcgacat gagcgattct ttccacgcgg gcgcgccatg
     1321 tctcaggcgg cgcggggacc gccgtggcat gatatgggcg tatgggcgtt atctgagctg
     1381 tatgtgtggg cacgccgcaa gaccggcttc gcggtgacgt tgagtccgtg ccgccgggtg
     1441 cgctaagatg cgggctgttc gcgggcgacg cacctgctac gctttcgggc ggcggaggct
     1501 gtgttgttca aaggcccggt caccaccgat tggtggtgac cgggcctgat tacttcttac
     1561 tcacgagacg gatcacctcg ccgcacacgg tcagccaccg cggagcgtgc gagtgagcta
     1621 cgtgagtgga cggggcgacg cagtgaaggc gcatcggaaa accttgatga cggtggaagt
     1681 gaggctagcg aaggggagtg ttcgatagca ctcgtgagat gcgtgccgag gagcgagcgg
     1741 gagactaatg caagatactc ccgctgtcaa tgacaatttt cttgcatccc caaagacacc
     1801 gaaatgacga gtttggttcc cagcggaaat caagaatccg gggatagaca tgggtggggc
     1861 gggtgtgtcc catcggggcg ggcgcctcaa ctgcccgccc tgcgcggcgt caggtgaagt
     1921 gtccggcgcg gcctcacgcg agggacgtga gtggatggcg gcgggggaag gagtagcccc
     1981 cgccgtcatt atttttgtgc aaagcccgac gacgccctga cgggcacgcg tccgataaac
     2041 tagctcacaa agaggcgtga aagaccccct agcgggaggc ggcttgaccc ccgcggtgtg
     2101 tttgggcgcc gggtgagggg aggggtctgc gacgatctcc atggggtatc aaccgccggc
     2161 cattttccat ggggtatcag actgacgcga gtgctcatgg ggtatcgaga ctttgcgagt
     2221 gctcatgggg tatggggcgc gtgggtgcct ttgcgtgttc cgctccaacg atctgtcatg
     2281 gggtatcacc cggtcaggta gtgctcatag ggtatcgagg atttgcaagt gcatataggg
     2341 tatcatccgc tcggaccacc gccgggtggt caagcggccc tcgccgcggc ctgagagcgc
     2401 atgctcgtcc acgcaccttc ggaacttcaa gaggtatgcc gccagcacaa aatctacctt
     2461 gattgctggt tgatgtttga acatgccagc gcgaacaaag aggcatggac tttaacttca
     2521 aagccggcgt tggtgcgcgc gtgcaactgt tgcgcgccca tcttctctaa ctgcgagtta
     2581 agcgtctcga tgcctttccg atagcgcttg aggtcgaact catctgccca ttcatgttgc
     2641 gccatgttct tgcgccgaat ggggatgact cgcaccccgg tctcagccaa gatcgccgcc
     2701 tcgtcaggcg cgctgttgta ggccttgtcg ccgaacacgc acgcacctgc gggcaacaga
     2761 aaggtcaact cgtgcagagg cgtcaggtcg tgataaccac cggcgacgat ggcgaagctg
     2821 accggcagcc cttcgggcgt gcacaccaga tggagtcgcc agccgaagaa cttctcacgc
     2881 ttcgcggcgc agtagccaca gtagtcgcgc ccacgcacct tcttgttacg gcgcgcgcga
     2941 gcgcggaggc agaccggcag cggcatactg tcaatgatga aggccgcgcc cgaagcgaac
     3001 agttcgccga gggcttccag ggtcaacgcg agccagtcgg cgacggcgtg cagcctccgg
     3061 ttcaagcgcg aggtgctgag acgctccgag aggtagccaa gttcagtcaa gacccacaag
     3121 gcgcgctcgt ggttgttacg gaaatacttg gcagcaacga ccgccaccag caggacttcc
     3181 gcatctgtga ctttcgccag agggtggctg ctgtgattca aagagcgcat cacgtcgtca
     3241 atgatgacgt aactcgttat aatcagctcg tcgttcatgc gtgtctccgg tttgagtgat
     3301 aggtttggtc accatcattc tcaagcctgt gccgcatgaa cggcaactag caatcaagat
     3361 aaaatctgta tggtcaaaag aaaaattata ggactccggg agagtacttc acctctccaa
     3421 ctttgaggtc tctatgacaa tacactccgc cggtcatatc gcgcctccgc ttctggatgt
     3481 cattggtaac ttggatggcc agggccgggg gtttgccgca tttctgacac gtcatagggt
     3541 tttcagatcg tccccgtgct ggcgtgtatt ttccctgttg ggtggggttg ccaccgcgtt
     3601 cgaaaatgct agggtttgct aggggttttt ctttccaaag gctagctttt gctagccttt
     3661 tcttgggtct tgaaggtacc tattttaacc acctcggcgc gccctaacgt cggacgttgc
     3721 atacacttag catactagaa gactttccct gccgggtgtc acacactctt gggtttttgc
     3781 tagctttttc tactcgtcca gactctagga tttgctaggg ttttgtcggc cgctgttttc
     3841 acacagtcgc gagtggcgcg cgaacgtctt tgccggccct tctgtgccag gtctaatttc
     3901 tcttccgcgt tggcgagcgc cgccagcaag tcgccagatc gccacagact agcgggagtg
     3961 ggacggcggc gagaaactcc tccgtgggtc atatactgtc tcgtccctta tagcaggtca
     4021 ccagccgcag aggctcatag gtatcgttca acatggggta ttgactttgt tagtgcggac
     4081 aaacgctgcc acatcaccat gctcggtgac accggagcag gcacgcaaag ctcttcacgg
     4141 ccgcccgtgc ggagccccag cgagggcggg ccggagtcaa ccgtgaagtc ctaaatgtta
     4201 agttttatta agttctccgt cgctgctttc tccgcctttt ttctaccctt agttttgagc
     4261 ctacccactc gtctcctgac acttcggaaa taattcccgg ccatagcatt tttcagtaag
     4321 cacatatgcc gacagtcatc cgcctttttt taggaggcga agtttcgccg cgcgggcctc
     4381 agcgtgtgcg gcgttggcgc ggcgcatggc ggccaggtac tgagtcacgg acgggttagc
     4441 tggcaacttg gcgagttccc gtttgagatg ggtcatgctc tcgcggtgaa gcctctcgcg
     4501 gagctgtcgc gcccgccgct catcctcctc gacctgcgcg gggcttctga agaacagcgg
     4561 gcggtacttc gaatagcgcc cgtgcttgaa tctcgggtgt tccgctccgc gaggcgactt
     4621 gccgccgtgc aggttgcacc ggcgtcggtc tggcgccggt cgacggcggc aattcccccc
     4681 ggcgcgtgtg cgagcgccgc agaggtcctc atggggtatg ctctcgttca tggggtatcc
     4741 aaaatcagca cttcgctacc cccgtccgcc ggggaggcct gaccggggcg tgccgccctt
     4801 aaggatgggg cgaccttcca cttttctacc gtcatcataa ttgcggctga gccgtcatag
     4861 ggtatcccgc gccagccctt tttgcggcca acttcggcca actcattatt aaagctctcc
     4921 gatttctccg gtttgacccc tccgagcacg taaagttatg tcggattctg ttaagtcgaa
     4981 atgttaagtt ttactaagtc ggacgcgcgc cggaaatacc gacaattatc gacctcgccg
     5041 cgggcgaaat ctcgggtttt ctcaagtttt ccaataacaa gttttactat agcggtttgc
     5101 agttgagtgc gaccgtttag aggcgggcta cggccgccgg ctcaagattc gagggttgtg
     5161 acggcgggca tcgcccgcct ctaaacggtc ggtggcgcgg ttacacccaa ctgcaaaccg
     5221 ctataagttc tgaattgcat cagtgattta catcactttc catcataccg gcagaaatgt
     5281 aaggttctgt caggtctcaa atgctagctt ttgctagtcc gagcagaggg aagcaggcgg
     5341 gcagggtgta gcacaaaggg cgtcgaattc ttaggttttc ttaggtattc aagcgcccga
     5401 aagttctagg tttatctagc atgtcgatgc tagtaaaatc gccgccccct ccagctatgc
     5461 aaaatcgtga agaagtgtga aacgcagggg gccagaaggc gatccagcat gaggtatcga
     5521 cgctgggcgc gtagcatgag gtatcagcgg ccgaggtcaa agcggaagaa aaccggagag
     5581 gtcgcgccgg cacgtcatag ggtctccaag acctcatagg gtttccaaag acacgcccgg
     5641 gcagagcgac ggccaggggt tccccgctag cctgggttcc caacccgcca cgatcttcga
     5701 gcgccaccac cgcggagagt caaagcgacc gggtgaaggc gacgcggctg cggcacatcg
     5761 ggctgaaatc ggcgagccgc tggggtggtc acgcgcgacg acccacagca ttggcgtgat
     5821 gatgttgcag aagccggagc cgcgacccgt gaccatctgg gcgcaacgcc tcgtcatact
     5881 agaaaccatc cttggcccgt tgcaagccgc gttgctagca ctcgccatcc ggcgtaaatt
     5941 catgcgttga agattctggt gatggacata aaaagtatta tcggacgtaa taactctagt
     6001 ttgtgtggcg acacgttccc tgattcagca tagggcattt aaagcgcata aagcgcaaac
     6061 ggatgggcca ggacgttggc ctgacccggc gtccggctcc tgaccccgtg gccggtccat
     6121 accctgcccc gtgccctagg ctggcagatg tgttgggccg ttttcgtgtc atcagttcgc
     6181 ccggagtgca tttggcagtc atgctctcag acggcgactt tgagtcctag tcgccggtca
     6241 agcagttttc accgctaaat cgctcgagga ataagcgtcc caggtgcaac cggttgcgcc
     6301 ccgacgtcgt ctaagacacc tccccgacgt gttccgaacc ataatccaaa gccttatcta
     6361 ggagtaatca tgccccgccc tcgcctcctg cgcccttacg ctgcccgcct gctcttgttc
     6421 acctttgtcc tcacgctcgc cgtgtggtcc gccctcccgc ccgggtacgc ggcgaaggcg
     6481 ctaccgacag ccccggcggc cagtaactac gtcgtcacca ccacgggcga cgccccggac
     6541 agcaacacgg ctgacggcgt ctgcaatgac ggcgcgggaa attgcacgct gcgcgccgca
     6601 atcgagcagg ccaacgtcga cgacacgtct gacatgatca ccttcgcccc gggcgtcacg
     6661 ggggtgataa gccttttgac cccgttgccc gacttgacga gcatgaccat cacgggtccc
     6721 ggcccatcgc agctgaccgt gcgaagggaa gatgcggcgc ccagcacatt ccgcgtcatc
     6781 accctcctac ccttcaggtc gctgaacatc tcgggcatca ccatctccgg ggggtactcg
     6841 ggcaggggcg gaggcatacg agtcgagggc tcgcccgtct ccctaacgaa cgtggttgta
     6901 agcggaaaca cggccgacgg cggcatcagc tttggaagcg agggccgcgg cgcgggcatc
     6961 tatcaatcgg ccggcgtact caacctgata aacagcacgg tgagcggtaa cgtcaccaag
     7021 aatgcggcca acaatagcgg ccatcacggc gcgggcatat acaccaccag caacctgaac
     7081 gttacgggta gcaccatcgc aggcaacaga attgagaacg ggggcggggc gggcggcgtc
     7141 ggcctgggcg gcgggatcta cgtcgcttcc ggctcggtgg cgctgacaaa tagcaccgtg
     7201 agcggcaaca gcgccggcgc cacagggggg caaggcggcg gtattttcaa gcagggcggt
     7261 acggttatgg cgaagagttg tacggtgact gagaacacgg ccgacagtgc gggcggcggc
     7321 atcgccaaca gtgccggcac cttcaccctt caaaactgca tcgtagccaa caacgccgcc
     7381 aattccgccc ccgacctctt cggagctttc agctcgcagg gctacaacct tgttagggat
     7441 gtgaccggcg cgacaattac tggcgcgacc gcgcacaaca tcacgaacct cgacccgctc
     7501 ctcggcccac tccagaacaa caaaggccct acgcagacac acgcactact gtctgggagc
     7561 ccggccgttg acgccggcga cagcggcgga ttgacgaccg accagcgcgg cttgcttcgc
     7621 cctatcgacc tgtctgctgt caacgtctcc gatggcgctg acatcggggc ctacgaggtg
     7681 caggccgggc agatacagtt cggctcggcg acctactccg tggcggaagg cgccggcttc
     7741 gcgactctta gcgtaactcg tactggaggc gactccgtag ccacctcagc gaccttcgcc
     7801 acgtctgacg ggtcggccac cgccggcgcc gattacgtgg gcgcgaccgg aacaatcacc
     7861 ttcgctgccg acgaggtggc gaaagacatc accgtgccga ttaccgacga ctccctcatc
     7921 gagtcggacg agacctttac ggtgaccttg agtgcgccga ccggcggagc cacgcttggc
     7981 actcggtcgg cagccgccgt gattatcgtc agcgatgacg tggggcccgc acttcccttc
     8041 gtacagttct cggcctccga agcgagcgtg ggcgaggcgg ccggcgcgct aaacgttagc
     8101 gtccagcgca cgggcgacct gagcggcacg tcttcggtct cctacgctac ctccaacggc
     8161 accgccaacg acaggtccga ctacacggcc gcttacggca tgctcatctt cacccctggc
     8221 caagagagta agacgattac tgtgttcgtc accgacgacc gctaccagga accgaacgag
     8281 accttcacca tcacgctgag cggcgcgtcg ggcgcggccc taggcctaaa tcaatcggta
     8341 actgcgacca tcgcgagcga cgattcagcg agtggcttga gcccggtcaa gggcgggccc
     8401 tcgttcgacc cggagttctt cgtgcgtcag cactacctcg acttcctcaa ccgcgcgccc
     8461 gacgccgcgg gtctggcctt ctggtcgaac cagacgacca actgcggcga cgccgacctc
     8521 ctcgtctgcc ggatcaacgt ctctgccgcc ttcttcctgt cgggagaatt tcaggagacc
     8581 ggcttctaca cgatccgggc gcagcgagtg gcctacggga agaagtccac ggaggaggcg
     8641 acgcgcgtca ccttcgcgca gttcatccgc gacgcgcggc aggtgggcga agggttcgtt
     8701 gacggccagc ccggcgcagg cgccgtgctg gagaacaaca agaacgccta cgccctgcag
     8761 gtcgccggcg aggcggcctt cgcgcaacgc ttcccgcaga cgacggccga cgcctacgtg
     8821 gacgcgctct tcgcctcggc gggcgtcact gccacggcgc aggagcggca gaacgccgtc
     8881 aacgcctacg gcggcggggg cgcggcgggg cgcgcggcga cgctgcgctc ggtggccgac
     8941 tccgcgagcg tacgtaacgc agagttccgg agcgctttcg tgctgatgga gtacttcggc
     9001 tacatgcgga gggacccgga ccaggccggg tacaccttct ggctgggcaa gctcaacgag
     9061 ttcggcggca actacatcgc ggccgagatg gtcaaggcct tcatcacctc gggcgagtac
     9121 atcaaccggt tcgggcagta ggcccgttca ggcaggaaat caaaagcgga aggggcgcgc
     9181 tcggccatgg atggcggcgc gccctttcct gttccgatca ctgtcctcac ctcagcaacg
     9241 agtggacacc tccctcagcc gtcacggatt catctggggc ggacgtaatg acgattatcc
     9301 gacgtgataa cgtgaaacat cgccggaatg agagcctgac cacaagatgt aggctgtcga
     9361 gatgtgcaaa aactgacggc gggagatgtt tcacgcccat tttgaaattg cgcgtccgac
     9421 cacaacatct tgcgccgctg ttttgattgt taagagcagc ggcgtgtacg gccacatgat
     9481 gttgtagaac acggtagggt tgagacagga gagtcaactg gacgttataa atattgtccc
     9541 gttggatttt attttgcttt gatttattaa aagcacaatg ccttagaatt gttagtagag
     9601 atgaaggtac agtttcggga atcccgccgt gccgccctat caactcagga gcatcgcagt
     9661 ggcccacacc gccgggtagc agccgcagaa tgcccaaacg aaccgattca gtcccatcac
     9721 tatggcgcag gacacgtgga agacgactcc gacagagagg agcacgagca tcagtggctt
     9781 gggtacaaag agcacgagag gaaacgtgat ctcccagccg atcaccgacc agcacaagag
     9841 ccagtcgagc tttgggtatc ttcccagcag cttcgcaaga ccggggagac catagccttc
     9901 ggtcgaaagg attccggtca tcgctccccc gttccgccaa atcggcgacg ccgccttagc
     9961 cacgcctgat gcgaaatacg cgaggaccac ttgggccgcg atgaacgcat cgccaagacg
    10021 ccgagccgag tctgttccgc cagcaaggag cacgagaccg aacgtgacga gcacgatgaa
    10081 ggtgagttgc tcagcgccac taccgccgat ctgccgccgc gaacgcacgt aaccgttcgt
    10141 gacgagacag atcagagccg aatacatccc ggcgcgagtg tgcgttccct tgatgattac
    10201 gactaatgcc gacaaccctt gtgcagccat cgcaagcatc atcgcggcgc tgccgccggc
    10261 cgtcgcaccg atccttactt gccagcgcgc accgctggtc aggatcgcaa tgttagagcg
    10321 agagaacatc ccatcgtcgc cgaaggcccg gcgcagcttg aagatgtcga acgcttcaac
    10381 gatcaatccg acgaatagaa ggcgggaaac aagaagctca ggatgagtaa acatgagtga
    10441 ccgagtcagc ggtgtcgtgg acttcggaga ggaacacgac ctcgacgcga cgggcgggga
    10501 aatctgtgga ggtgcgcacg atagcgaact ggatcgagga gcatcccgtc cgtctaaatt
    10561 gggcgaggac aatgctcaat agagtgaggt acggcgaaca gagcagcgcg ctgcgatctt
    10621 cccgttttgg gcgcacgcga agaagcgaac cgacaagatc ggtgaccgct ttgttcggaa
    10681 taagctccgg gttcactaac catcggctgc tcgcaggtgc aaacccgaag tgcaactccc
    10741 gccacacacc ccattcattg ccatgctcct cacgccacag caatcgtgaa tcggcgcggg
    10801 ctggattcgg cgcaaaaaat gtccagagcg ggatcaatcc caacgggata tgacggcgga
    10861 gtcgcaatgt ccatgcgccg gagcgaaact gattgcacac actgatcgcc caccaaacag
    10921 cgaatatcgc cgcgattgtg agcaacggga tatccatcac ggcccagcct ttcagccgcc
    10981 ctgaacgatc ttgcctctcg cattcagcag ctccttcaaa gacgtgcgcg agctaagttg
    11041 gccagcgagt tccttgatac gcgcgatgat ctcccggtcg cgctcggtca gttcagtgag
    11101 agggtgatga gcatctgccc acgcagtcgc ccattgctcg cgcgggttgt cgtctttggc
    11161 gatcctgtcg ttgatcttgt gaccagcggc ctgcaacgcg gtgatcgtca gatcaatcgc
    11221 ggctaattcc atgtgggaca gactggtctt ctttattggt ttgtgcggac tgctcttctt
    11281 tccttgtttg tcttgcattt aatcctcctc aggtgttcgg cactcgacgg gtagcgccta
    11341 tctggtatga ccggacgttg acgtgagtgt ccagtgagac gttagaagtg gggccacaag
    11401 cgagggtaca atatgactat cataactagt tgtcaataga tgtattgatg tttatggcct
    11461 atggctttat ggtcaaaaac atcaatagaa cttaaagtta tatgaaagtc tgattttatg
    11521 ggcaaagcct ctaagacata agaatggtta tcggatttca gtgatgtaat agaacattaa
    11581 agataaagag ccaggtatca gacttggctc tttgtctact cctatacttg atattgggcc
    11641 ttaagctacg ttgacctgcc gccctccctc cgccgccacg ttgatctgca ctttcgggac
    11701 gcgagccgag agcttccgca cgcgctccaa cgactcgcac gcccgcgtga acctcttctg
    11761 cgccgccgtc agccgcttct cccagtacgc gcccagcgtc agcgtgatgg actgcttcat
    11821 gacgttggtg tagcgcatct ccaagatcga gagacgaacc caacacacgg ccgcgtgttg
    11881 gatcaaagcc ttttcgagtg gcggcgcggt cttgattccg agatcgtctt taaactccct
    11941 ggcctgtagc gtccacaact ctgcaatggc gtcggtcgtt aaggtcgaag cgtcggtcgt
    12001 caacaggacg gcgagcgccg catcgagcgg gtcggtcacg tcccgccacg gcgccgcctt
    12061 ctcgccttca cactccttta aagtctgccg gaaggcttcg agggcgccgg ccgccggtgc
    12121 ccgcttgttc gcggcgctca gcgtactgat gaagcgttcc ctgagcgtcg ccgtagcctt
    12181 caccacatcg gaccgtttcg ctttgaccat ttcgtcaccc cgccatctgc ttgagtacgg
    12241 ccagcgaccg cgcgctcgac gccctgcgcg ccgactcccg cgccgcgtcc gcccgcgcca
    12301 gcaggccgcg caccctggcc agcgtcacga cggagcgcgt gaaccgccgc tgcgccagcg
    12361 tcagccgctt ttcgtaatgc tcggcgaggc tgaccgtgta gctgcccgat gtgttgcgcg
    12421 agtaaaggtg ctcgatcatg ccgagccgga cgtggcacag cacggcgtgc tcgatcagga
    12481 tgcgctcggc cggcaacgcc tcggcgtagc ccaactcctg tttgagaagt tcggcgccac
    12541 gtctcaccga ctctttggtg acgtagccga gccagaactt ctcgaaagcc aggtcgagcg
    12601 cctccgcggt caggtcgccg tggcggcgcc actcctcggg gtcggcgtcg agctgcgcgt
    12661 cgtagtaggc gcgggcttcg gctgaggggc tgtccttcat catcgcctcg gcgtacagtc
    12721 ccgcctcgcg ttcccacttt tctttctcgc gctcagccag tgagacgacc cgtttagcct
    12781 tcggcattcc tcttcaacct ccattgcctg tggtgcaggt ggtagcccgc tgactccagc
    12841 accgacttgg ccgccgtctc ggcgacgagc cacgaccgct cgaccagtgc ctccacccgg
    12901 tcggcgtgcg ccagctcgcg ccgcacctct tcgcgggagc gcactcgctc ccgccgctcg
    12961 tccttatccc gcgcctcggc gagcgcgccg gtcagtcccc cgccctcgta gacggagaag
    13021 acgcgggttc ccttccgcac cttgcggtag tagtagcggc gcccgcttct ctcttcccac
    13081 ggcataagtt ttttgtgcta cggatttttc cctgccccgc ttgcctcata cttggccagc
    13141 ttccgctcga tcctcttcgc ggctttgatt cgcgcgcgca gcatgacgcc tatcaggcag
    13201 tgcttggagt agaggccgtg cttgtaattc gggtgggcga tgccgaagag gaatttgccg
    13261 ccgtggaggt tgcagcgagt acggcccgga gtaggcttac gtcggcactt cccgccggag
    13321 cgtgtgcgcg caccgcaaga gctttcatgg ggtatgacct cgttcatggg gtatcccaaa
    13381 attcagattc ttggcgactt agaataaggg ccgaccacca gcgcttatca gaggcgcccg
    13441 cgaacgcgcg tcatttctct tgtcgggagc actcctcggg ccgcatcttg tcagagaaac
    13501 gttgtttcag gcaagaaata tctttataat ctgctcacaa agttcggatt tcacgcggct
    13561 ggaggccaag tatgtgtgga cgattcacgc agcgggcgga ccggaaggcg ctcttgaagt
    13621 cattcgcggt ggcggaggtg ccgcacttag agccgcgcta caacatcgcg ccgacgcagg
    13681 acgtcctcgg cgtccatcaa tcgccggatg ggcgcgaggc gacgttcttc aagtgggggc
    13741 tagtgccgtc gtgggcgaag gacacttcga tgagcgcgcg cctgatcaac gcccgctcag
    13801 agaccgtggc ggagaagccg gccttccgcc aggcgttcaa acagaggcgt tgcatcatcc
    13861 cggccgacgg cttctacgag gggcagcgga cggaaggcag gaagcagccc ttcttcttcc
    13921 gcatgcgcga cgagcgctcg ttcggcttcg cggggctgtg ggagcattgg gagggcgagg
    13981 gcggcaaggt catcaactca ttcaccatcc tgacgacgga agctaacgag gtgctgcggc
    14041 ccgtgcacga ccggatgccc gtcatcctgc acccggagga ttacgaattg tggcttggcg
    14101 cgggcgcgcg ggaactcgat ctcgtcaaag agatgctgcg gccttacacg tccggggaga
    14161 tggtgggtta ccctgttggc gtggctgtta acagcccgcg caaccagggc gcggggctca
    14221 ttgagcgtgc ggccgtgaac tcggcgtaag gcccggacgg aggagatcta tcatgaccta
    14281 cgcgttgtgg atcgttcagg cgctgctcgc gctcctgttc ctgttcgccg gcgggacgaa
    14341 attggtcata ccgcccgacg tactggcgtc gatgggatcg ccgaaccaga taccgctgcc
    14401 ggggtggttc gtccgcttca tcggcgtggc cgaggtgctc ggcgcgctcg gccttttact
    14461 gccggggctg ctgcgcgtca agccatggct gacaccgttg gccgccgccg ggctggtcat
    14521 catcatgatc ggcgcgacgg cgctcacgat ggcggccgac ggggtcgcgg ccggggtggt
    14581 cccgctagtt gtggggctgc tcgccgcctt cgtcgcctac ggccggtggc gactggcgcc
    14641 tcacgcggcg cggcgggaga tgattcggcg gaggtcgagt tgaggacgac aagaacaatt
    14701 aaagggagag tagatgacga ctgaaaacag ccgggccgct gacgaagccc ggatacgcga
    14761 gctcatcgag gaacgggtca gggcgatccg cgccaaggac gttgacgcgc tgatgagcaa
    14821 ccacgcgccg gacgttgtga tgttcgacgc cctcgacccg ttgcggtacg tgggctcgga
    14881 ggcggtcaga gagcgcgccg gtcagtggct ctcgtggtat caagggccgg tcggctacga
    14941 ggtgcgcgac ctgagcgtca cggcgggcgg cgaggcggcc ttctgccatt acctttacag
    15001 ggtcagcggg acgatgacca acgggagcga agtcaacatg tgggtgcgct caaccgtctg
    15061 cctccgcaat acggaaggcg agtgggtggt cacgcacgaa catacgtccg tgcctttcga
    15121 cgcggagagc ggcaaggcgt cggtagacct caagccatag cggcgcgcgg gcggggcgta
    15181 aaccgtcggc gacccacgta aggggccaca gtcgggtctg atcaggatta gacggacgaa
    15241 ccttgggtct aatcacgaag ttgggcggac gatccgggag gacagcgcgc gataaccctg
    15301 aacgcggtct ccacccctcg aaaggaacat cgcaatgtcg aaggttgtcg ccatcatgtc
    15361 catgtcgctc gacggctacg tcgccgacct caacgatggc gtggccgaag tgttcgactg
    15421 gtacatgaac tcaggagacg tcgagttcca caccggaggg tcggacccca tgacgttcaa
    15481 ggtttcgggg ccgagcgccg agcaccttcg cggtctctgg tctgaacttg gtgccgtgct
    15541 caccggtcga cgcactttcg acgtcgccca tggctggggc ggaaatcacg cctggggacc
    15601 ggcattcgtc cttaccgacc acatccccgc cggatggccg cggcccgact cgactgtcca
    15661 cttcgtgacc gacggcatcg aaagcgccgt gaaccaggcc aaggccgccg ccgccgggaa
    15721 gtccgttggg gtccacggcg ctgacaccat ccagcagttc ctgaatgccg gcctcatcga
    15781 cgaactttcc atcgacatcg cggcggttct tctcggctcc ggagttcgac tcttcgacca
    15841 cctcgccggc acgccggccg tcctcggcaa cccgacggtg atcccgggag tcggcgttac
    15901 acacctgcgc tacccggtac gcaaggcgta gtcctacccc acgcctttct cgcccggcgg
    15961 tgagccttga cgttcacgct cgagtgcgcc aacgaggtgc ccacaagtag cgcgccgccc
    16021 aacaaggacg ttgcagcgac cgacgcggtc gtccgctgcg ctccctctcg cgttcgccgc
    16081 tgaatgcccc tatcgttcgg cggctcattc caatggagaa gtgacatggt caaaccagct
    16141 aagaacacga tttgcctttg gtacgacgga gacgccgaag acgcggcgcg attttacgcc
    16201 gagacctttc ccgattcatc cgtcggggcg gtgcaccgcg ccccgggaga ctttccttcg
    16261 gggaagcaag gggatgtgtt gacggtcgag tttacggtga tgggaattcc ctgcctcggg
    16321 ctcaatggcg gacccgagtt caagcacaac gaagcattct cgttccaggt cgcaactaca
    16381 gaccaggccg aaacggatcg gtactggaat gcgatcatcg gcaatggggg tcaggagagc
    16441 gcgtgcggct ggtgcaagga caaatgggga ttgtcgtggc agattacgcc gatcgccctg
    16501 acggaagcga tcaccaaccc cgatcgggct gcggccaagc gcgcgttcga tgcgatgatg
    16561 cagatgaaaa agatcgacat cgctgcaatc gaggcggcac gccgcggttg acgcatgctt
    16621 tgattagcgc tctggcctca tgccaccgaa atggcggatt cacccgagcc cgcatagctt
    16681 gggtgcatcc gtccttcttg gtgccgtcgt gcgggccggg tgatcctggt cgttcggtca
    16741 gctactttta cataattgcc gaactcggac gcacttcgct gcgctaaacg cgggtgcggg
    16801 cagatagctc gagagccgac acataagagt tatgcgtcgg ccctcaagcc atttgtttta
    16861 ctggcaaatt cttaccgttt gggcggttgg tgaataataa tgacatactt atgattgatc
    16921 aatccaccga acgcacctcc cctaatttcg ggtggacatc ttgtaagggg ctagagtagt
    16981 taaagcgggt attttcgcag tagcttccac aactcagcat acacgttgtt ctttctcaag
    17041 ttgaatctat attcgcactc cttcaagtgc caatagaact tctccggggc gacgccatga
    17101 aactgctgga gccgatgctt agcataactc cagaagctct cgatgccgtt gacgtgtgcc
    17161 gtgccacgca cgaactcgct atgcccgtgg ttgactctga agtgcttggc atagcccacg
    17221 tcaaccaggc cgtcgtagcc gcgcgaggcc gtccgagttg atgactgctt cgggcgcgac
    17281 tctgccgcga ataatggcct gtaaggtgac ctttttacag tccggcacga tctccgtgta
    17341 gacgcgcccc tcgcgcttgt gcagcccgaa gacgatggtc ttgccgcccg ccccgcgacc
    17401 gcgcttgcca cgcacgcgac gagcgccgaa gtaagactca tcgacttcaa cttcgccgga
    17461 cgagagcggt gaatcacgtt cgcattcttc ggcgatgcgc cccctgattt tgagaaagat
    17521 cgtggtgaca ctcttacgcg tcaagccggt cagttgacgg aatttcttgg cggaaattcg
    17581 ggaacggcga taaaacgggt tcggtcgttc catctcagta gtttagcacc tcccagaaag
    17641 tgcttaacta ctctagcccc cttgtaatta tgctacggca aacggccatg aggtagggac
    17701 gttgctgggg cgactattat ctacgcccgg acaccgtatt ggctccaaga ccccgcacgg
    17761 ctttggtcgg tagagtcgta tcagcgagcg tgccgattcc tatggtaggt acacccgtac
    17821 gctcgggtct tgccgggagg tgagagcgct gctcgccatg acaatcaggt ggtgggtcag
    17881 tttgcacctg aaaactatat ttttcagccg tgcgcgcgcg gcctcatgaa atgtcttagc
    17941 gtgaaatcgc atacagattg taggcgagag tatttagagt gatcgcctcg ggcaatccgg
    18001 ttgcggagca gtcgcggcgg atttgaaatt gacccattaa cgacaatcag caataattga
    18061 tatctaccag ctaagagcat atcctcgcgc gcgtctaacg catcattttc gtgctgagga
    18121 actccccgac ccgtcggccc tcataaacga gtatagatcg caaccgcgcc cggcggccgc
    18181 agcactagca ggccggcgga cgctccgtcg gcaggcgaac gacgcacgcc acatcctaac
    18241 ctgggtcgtg gatgttcagt gtgaatagcc cccgcgttac gcgcccgtcg cccgcacctc
    18301 tcaacctcat caaccccagt caggaggcgt aagcgatgca agactcaatc ctcttctgga
    18361 acgcggtcgc gctcgaagcc aaccgcgtca gccatagtga ccccgacaag cggcaacaga
    18421 acgggccgac gctctcgtcg agggcgatag cgattgtcca cctggcgatg tacgacgcct
    18481 acgccggcgt cgtcgggggc gcgttcgcgc gctatctgcc cgcgccgccc ccgccgccgc
    18541 cgcccggctc gtccgcccgc gacgccgtcg ccggtgccgc ccacaggacg ctgtccaggc
    18601 tttacagcgc ccagaccgaa ttcttcgacg cgcagctgag ctgcttcaac atcgctgacc
    18661 cgagcttcgc cttcggtgag gccgtcggcc aggcactgct cgacctgcgc gccaacgaca
    18721 tggacgcgcg cagttgcggc tacatgccct cgaacgaccg cggcaggcac cgcgtcgacc
    18781 cggacaaccc cggccagggc ttcgactcgc cgttctacgg ggcgcagacg agaaccttcg
    18841 ccgtcagacg cagacacagg tctgatccgc cgcccttcgg cgacgggggc gtcacaaagt
    18901 atttgcaggc cctcagggac gtgcgggcgc ggggcatcag gcccgacctg acggggacgc
    18961 tacccaaccc gctcttcaac aggcggagaa cctcagagca gaccctcacc ggcatctact
    19021 gggggtatga cggagcgaac cgcctgggca cgccgccgcg tctctacaac cagatcgtcc
    19081 gccaagtggc cgtcgagagg ggcaacaccg aggcgcagaa cgcccgcctc ttcgcctatc
    19141 tcaacgccgc gctgggtgac gcgggaatcc tgtgctggga gcagaagtac tgccacgact
    19201 tctggcggcc ggtcgtcggc atccgcgagc acgacgagac gctcgggccc gaggcaccgc
    19261 acggcacgat cacgggcgtc aacttcaacg acgccgaccc gtcgtggctg ccgctcggcg
    19321 caccgagcac caactcgccg ggcatgaaaa acttcacgcc gaacttcccg gcctaccctt
    19381 cggggcacgc caccttcggg gccgccgctc tgcacatcac ccggatgttt tacggcatcg
    19441 ccccgaacaa caagagcgga gaccccctct tcccgaacga attcgtctcg gacgaattca
    19501 acggcggcag ccgggacaac gacgggacgg tacgcccgcg ccacacgcgc ttcttccccg
    19561 gtggcctgtg ggacatgatc atcgagaacg ccctcagccg catcttcctc ggcgtccact
    19621 ggatcttcga cgccttcaac ttcaccgcga gcggcggtaa cctgaccccg tctttaaccg
    19681 acgaggacat cggcggcgtg ggcctcggcc tgcgaatcgc cagagacatt ttcgcgcacg
    19741 gcggtggcag cgccggcgcg ccccagatga ccccggccgg cgtcaacccg ccgatcatca
    19801 cgcccgaccc cggcccacct gcgccgcccc tgccagtcca cccggcacag ccgacggcgc
    19861 agagaaactg cgtcgccgcg agagccgccg ggtcgaagaa gtcgggcaag cggggcgcga
    19921 aaaagtcgag caagaagggg gccactaaag gtgctgcgaa gaaagcgggt aaaggtgcgg
    19981 cgccgcccga gatggtcggt cagcagcagc ccgcgtggcc gtccggactc tccgaacaga
    20041 gcgaggatca acccggaaac acgggcccag gcgcgtggcc atccgggatt tccgagaagt
    20101 gatgcccatg cagttggccg tcggctgtgg gttttcgtcc ctcgcagccg acggccaaac
    20161 ttagagccgt ttatagctct gtctcctcta attggagtct attgcctctt gcacctagtt
    20221 cgcgtctcct cgctactatt cctcatcata tccaaggatg ttgacggccg attgctccaa
    20281 gatctcccgc ccgtggtcgt atctcaccac cgtcttcagg ctgcggtgct tactcagcat
    20341 ccacacagat ctataagtgt gcaagggttc acgtccggct ccttcgtgaa cccttaacac
    20401 cgacgcccaa ggagaagcgc ttccacattt ggacttgcaa atgtcccgcc gaaaactctt
    20461 gacacccaaa atcgggagca gtataaaccg acgcatcgaa cacgggcggg ccctctccca
    20521 cgtaaagcgg ttggtgaaat cctcacggac tccgtcaacc tcatttcaaa tgacactcgg
    20581 agtaatctaa ataggagaca taggtcatga aaaagattgt gtgcctatca gtctttgtca
    20641 tttcacttgt ctcgatgctc cagcatccga atgttctcag ccaggggaat cctcaaagcc
    20701 ctgaacaaat cggtaagcct tgtggggcag accccgcgtg tgaccccaac agcccgaaac
    20761 agggcacttt taaagcgcca atcaatactg acggcaaacc aaaagggtgg gtcgtaaagt
    20821 tctaccacaa aaagcatcat caaaaacgtg agtcctgggt agatgaagcc cacgggcgtg
    20881 gcttaactca cattaaaaag cacgctaaag aatttgatct tgttgatcct catgcggagc
    20941 ttgagctgct ctcagttgag agggatgatt taggtatcac taacgtcctt tttgagcagg
    21001 tgtacaaggg agtgccggtt tacgggggca aactggcgat acgctttgac gcaaacggtg
    21061 aactcctcac ctaccccgcc gaagaaggcg agaggagagg aattgacggt caaatccaga
    21121 agggtaagga gcggaaaggg attgacggta aaatctatgc aactgccaag cacgtcgata
    21181 caaaaccccg cctggactcc gcgcaggcga ttaccgcggc caagtccgca ctaagctaca
    21241 cggggccttt ctccaaatcg ccggaagcag aactcttcat actgccacgt caagtcggga
    21301 attccgggcc tgccgacttg tcgggcggtg cgacgctggt ctacaaagtc gagcttttaa
    21361 tcgaggatgg agtcaggacg cccgagcgcc actggtactt cgtggatgcg catgacggaa
    21421 gcgtcgtgtg gcatttcgac gcgatgggtc aaggggcggg gcagagttgg tatagcgggt
    21481 atgtgtggat tcctacgggc tggtttgcct ggaataatac cggcccgcac tgcggactca
    21541 ctcaatggaa caacgggttc tggctatggg acggcagcgg ttaccgcggt aatatggaaa
    21601 cccttgattt gaagaatacg cagccggcct ataactttta ttactattcc atgatgcgtt
    21661 ttgacggggt tgatgatcgt tggggtatgc cgacggagta tggcgcgtgg ccggctccgg
    21721 actgtaactg gcagcgccag caggcgggcg tcgacacgca atacggaatg gcactggcgt
    21781 gggattactt tttggatacc cacgggcgcc ggggcattga caacgcgggc ttccgcatgt
    21841 tcagccgcat tcactacggg aataatttcg actgggctgc ctggaacgga tacaacttga
    21901 ccttcgggga tggctcttcg ggccgcccgt gggtgtctat cgatgccgtc gcgcacgagt
    21961 ggacgcacgg gataaacgag aagacggcca acctccacct cggcggcgag tcgggcgcct
    22021 ccgccgaatc tttcagcgac attttcggca ccatgatcga gttttacgcc agcaattggg
    22081 tcaacccccc cgactggctc gtcggggagg acttcttccc cggcggtaag cgcgacatgt
    22141 acaacccccc gagtaagggg caccctgacc attttaataa tcgagtgtac ccaggccatt
    22201 gttggcccga gtcggccaac gacaattgcg gcgttcacag caactcgggc atccagaaca
    22261 aagcgttctt cctgctggcc gtagggggca ctcatccgta cagtggcatc tcagtccccg
    22321 gagtcggcaa tgatgtggcg gcgcgagtct tctaccgcgc cctgacctcc cacctcctcg
    22381 ccaagccgta tgcgcgtctc taccaggtca gaaacgccac ctacaacgcc gccgtcgaat
    22441 tatatggcta ccacagcacc gtgggggata aagttgaaga ggcgtggagg gccgtcggcg
    22501 ttccgagtaa tcccatttat gagtcgtggt acttcgtcga gcagcactac aaggacttcc
    22561 tcaaccagag cccggacccg ggcggctggg actactgggc gaatcagatt aacaggaact
    22621 gccaccccaa cgatcaaggc tgcatcgagt ggcggcgagt catggtgtcc cgcgcgttct
    22681 gggaatccgg cgccttccgg gagcgtgcgg aggtgaagaa ctcgggtctt ctcaacccgc
    22741 cggggtcggc ccgtccctac gacaatcgcc agttcgtgag gtggtgctac aagatgtact
    22801 tgaatagaga acccgaccag ggcggctggg atttctggac aaacgacctc aacggacacg
    22861 gcgattacaa catcacgatc aacgccttcc tccgctccgg cgattaccat tacaggtacg
    22921 cattcctgta gacgcgggaa gcccgaatga agtgggccgg catccatgtc accgtgaaga
    22981 aagaggccat tccggtcggc ccattaatcc tctgtggaat cctcgcggcg ctgtcaaatt
    23041 gctcgccacg ccaggcgaaa gacaggggcg tcgctcaggc ccccggcggc gccctccgcg
    23101 gcaggaacgc cgtgttggaa ttgccgccgc aggcgcgggg gctcgcgctc cccaccgagg
    23161 gcgacatgtg ggtcgtggta gtcagcacga ccgccggctt ttcaggtgac ggggccggcg
    23221 gggtggccat cacttcgcaa gggggtgtgg cagcctggca cccgtccacg gagtaccgcc
    23281 acccttgccg cgcggagctc gggggcgaag cggtgcgcgc cgtggcgcaa tcgaccacat
    23341 cggcaagccc ggagcgatgg agtgcgagct acgttaaccc cgagcgtccg cagggggtcg
    23401 ccgacgacct cgggacggaa ttagtcttgt acctgcgcaa ggcgaatggc gtcgagaaac
    23461 acgtcagccg gtggcacgaa tccgcctcac acacccgccc gaaagacctg gcagcgcttc
    23521 acgaggcggc cctcaagatt caatctcaag tactcggcag ctgcgtggaa ccccctcaag
    23581 gttctaaagg cggatgaagg ccgtcggctc gcgggtagcg aggctgttgg cagctaccgg
    23641 caccgccaaa aggccgcacc ctgtagcgaa cgtctggagg gtagtgtcgc atgtgaaccc
    23701 ttgtcctgct ggttagcaca aactttccac cttgggccct tcgcacttca attcgcttcc
    23761 ggcaatacta cgcgatttca ttttttagta cgcatcccta aagaagggtg gcgcttgcgc
    23821 agcacccctc tcaaatgttc tttggtgacg tgtgtatatc gctgagtcgt ggcgatggat
    23881 gcgtgcccga ggaattcctg gactatcctg atgtccacgc cgttatgcag gagtagcgtc
    23941 gccaccgtgt gacgcagcat gtgcggcgtc acatgccgct ctatccccgc ctcctcccgg
    24001 agctgcgcga tgacgttcgc gataccttga gtcgtcaggc gctggcctga ggagttcagg
    24061 aagagtgccg gactccgcgt ctcaatccgt gtcctgactt ctaggtactc ccgctggagg
    24121 cgtaccgtct cctcgtcaac cactacggcc agccgatccc tgccgccttt ccctttaact
    24181 ctgaagaccg actccctcat gaaaaagtcc tgaacgtcga gggatgacac ttcacctacg
    24241 cgtaaacccg tcgcgaaaag cagatcgacc agcgccagat tccggaggga tcggtattcg
    24301 ggggacgacg ctctaaagga gtctaatcgg gctgcccctt cgttattaag aaaagccgtg
    24361 gtggaagaat tattcctctt ggcctgtgag aggagtttcc gcatctcacg cgcgctgagc
    24421 gcccggggca gttgttccac ccgcccgtag ctcagcttga cccgccagaa gggggactcc
    24481 cgcagcgagc ccttgcgcac ccagtaggag cagaagactt tgaggacaac catcttccgg
    24541 cggatcgaag tgggcgaata ccctcgctgg tggaggtgtg cacaccagcg ctcgatcaga
    24601 gccccgctca ccgacttcag gagaagctcc tcgccggcga atacgtagaa ctgcgtcagg
    24661 tctgagcggt acgcggcctt cgtcttcttc gtccgctcgt tagtagagaa atacccgctg
    24721 acaaactttg tgcacgcttg gtttagttgc ataccttgtg cgccaaatag acaccggcga
    24781 ttcttcttta ggatggaaga gcttcctgtg agctttgaaa gagggtggta ggtgaaaatc
    24841 tgggggagtc taacggggta agattttgat ttcaagtggc gggcgctcgc tgtataatcc
    24901 gggccactcc gttggcactc gcgtaagcga agacctgtgc cctcatacaa ggtggaacgc
    24961 gccagccata aacactaaat cagtaagggg aagctttctt aattaagcga aatcccctta
    25021 ctagttaaca acatatggat aactaggaat tatcaccagc ctgaggagac tttactttgg
    25081 tttcggcttg gccgcacagg atcagaccca ggatcggaat gacgattgcc gttgttgttg
    25141 cggttgcgcg agcggcggcg ctcgtcgtgg ctgcgtattt cgataattcg ctgtggctac
    25201 ccggcgaagg ggttgggctg ttcgagcatc caggcgtact ggccatcgtg gtgggcgacg
    25261 ccgttctctt ccttttgtgt gtgtacgcaa gtcggatgac gcgatcagtg gggagacggc
    25321 tcccgacaga tcggcgaccc ttggttcaac ggtatttcag gctgatcata cttcgcagta
    25381 tctttgacag gggcggaagt ttcagcaaga tgctgttgtt tctgtcggtg atcggcgcac
    25441 tctcccttgt gaatcagact atacagctga cagatgccag cgcctattac ggtcacgaca
    25501 ccttcgacag tatcttacat cgccgaagct tctgggtgat gcgcgtgaat ttgttcatat
    25561 catggtgcat cataatccca ctgttatcca gttatctcat cgcgcacacg aaggcgatcc
    25621 ggctcttctt tagaaggtgc gatgggcacg gcctcgttaa gttccagccc gggcaccccg
    25681 atcaacatgg aggctttacc ttctttggct ggctcgacac catctacgcg ataggtttac
    25741 tcaccgtact cattgaagtg ttcttattaa tcattaccca taggcgagtg accgccggga
    25801 atcttttcag cttgatgggt atcacggttg gcggcgtctt aatctctgtt ctttcaattt
    25861 atgaagtagt acgtgttgtc aggcgccaag aacggctact caagatggcg gcattcgtga
    25921 ggactgtcag gggctctcgc cagttaagtc aggattacct ggcgctcgtc tacgatgcca
    25981 agttcagtcc gtacagcagg accgcattac gtcttgccgt cgcgctccgt gccgccgccc
    26041 taattcctgt tgcgatccgg gcgatgacgt acatcacctc aaagctttaa caagaacata
    26101 caggcacaag tacccgaatg cggtggcggc cgcaagaata gccgacacga ccgtggagtt
    26161 gtttaatgag gctggcaaga tcccataagg caccagacaa ccgatgacaa ttgcaaggcg
    26221 cacaacgccc gcgtgtataa ggaccagttt ccgaaatcct ggtacaagtt ggtcgaaatc
    26281 accaacgccc actctaaagg catacaagca gatctctcga aacgtgaaac tccacacgag
    26341 aaacgagcca agaccgtact cgcgatcaaa ggcaatcacg gcggcgatat agaacgcgcg
    26401 atctgcgacg ctgtcgaaca gccagcctgt tactgtctta cccgtagtac gtcgcgccaa
    26461 gtacccgtcc aagtggtcag tgaattgagc aatcactgca acaacgacgc ttgcggtcag
    26521 cagcgaccct ggttctcgac gaaatagtcc aacgaagagc aatgccagtg gcacccgtac
    26581 gagggacaca atgttgcctg gattgaggat cgcgcgcacg ataattccta gttatccaaa
    26641 atccactcaa aactgtcagt atctcgaacc caataggcaa agcggcgggc ggcatctcat
    26701 aatgccgccc gccgccttta acttctgact ctttccgttg atctttacaa ggttctgccc
    26761 cgaatctctg gctcgacgta tctgtcggca ggggagactt acccactagc gtccagttct
    26821 tctaccggaa gggttcaatt ccggctcctt cgtgaaccca gatcaagctt acggcagagt
    26881 atgaggattg gtagtgagaa tgccgatttt cataagcaat tttcgataaa taaaacatta
    26941 aataaggcac ttgcgtagtg gttcagtttg aaagctgaat cggaaaatca gtaaaacgtt
    27001 gcacgcatga acgaacgttg actgtcaggc aggacgagag gataatttcg gcgcattggg
    27061 ggtgatttat ggaacagaag gtactgagaa gcaagaaaca catttccaag cgacggaata
    27121 cgtttaactt tgcgttcgca cctaagcagg gcatttctaa aattttgtct gattaacgaa
    27181 tccaccagcg ggttcaacaa aaaagtgggc aactaagctc caagtttgat atgttgagca
    27241 ctcggcatct ttgacaatca agcgtcatta gcatgcgcat tgaggcccag cagcacggat
    27301 gatctgtgcc acccgcgggg caatggtcga taaaacctcg gcgggccgaa tcagcgatgc
    27361 aatattaagt gcaattgcac ttaggcgcaa atgtcccata ctgaagtacc catcaggagg
    27421 aatctcctga tgggtacttc ggcgagaaga agaccacggc atctagcaga aaaactgctg
    27481 cggatcaggc aggcatttgg cgattcgcag aatgggctga taaggcgctt tgagctaact
    27541 gacgagatta atcagtctga tgtctctgcg ttcgagcgtg gcactcgcga gccaccgctg
    27601 tacgttttat tgaaatactc ggaagctagc cgtgtgtggg ttaatgtcct tatcgacgac
    27661 aacatagatt taccgaagag actacctagt aaagatatga gtaatggcat tcgcgtcgcg
    27721 caacatgcac aggccaaaaa gaagttgcgg cacacgaggt aattccccaa gtcgaacacc
    27781 cataaactca aggtgagttt tgtgttcact tccggcccct ttgtgtgccc aatgagttaa
    27841 aaaggtagaa tattgcctat tctaaatgta atatcatccc ctgtgtaaca tcatcccgac
    27901 tcggagtcga tatcaaacat tgagagccac aagacgccga cagcgtaaag ccgatttgct
    27961 tgcctcgccg gcagcagtcc actccgtcat tgagaaactg cgcttggcgc tccccaactt
    28021 aatcccacac aaagacaaag agttggtgcg cctactccgt gctgccaggc acgcccagcg
    28081 ctaccccgcg accgatacga agcgtggacg ccccggtaga tggaagcgtg aggatctgct
    28141 cagggtggcc gcacgcatgg gtgacatctt ggaccgtgaa acatcctcac acacctcctt
    28201 cgccagcttt gtcgatcatt acctgcggct gattgacttc ccttccgacg tgattgaggt
    28261 gcttgaaaat ggagatataa acctttttga ggctgaacaa ctggcgcgcg tcaccgccga
    28321 acggctcgga gttacgacca gccaagctaa acgcacacgt gctgcgctac ttgcctccca
    28381 tttgcaaacg aaagcctcgg gcgagaagct tcggcagcgg gtcaatgaat tattccatac
    28441 ttcatcggct gaagcaggag agacaagcat tgaaagcgct gaattggcta ccgaactgga
    28501 ggatttagag gatttcgacc cctatgattc tacacacctc ttttgggagc agcttaagca
    28561 actgggcttc gccttcaggg agattcggcg tgaggatgtg acggatgagg agatcgagga
    28621 gctactgaaa gcgagcgagc cgattctggc gatcctaggt aggattcagc ggcgccgaga
    28681 gcagacgaaa attatcaaag tgaaactctg atcggtgtct gtttagtaaa acatgtgaag
    28741 atggcctaaa aatcggcctc cgtttagcaa aatatctgaa gatttttgag gagccgaaat
    28801 gcccgttata agttattgat ttatttagct atttgttcaa tttacccctt tcactggttt
    28861 taccccctgt ttagtaaaaa ttctgaaggt ttacatagga gatgtgaaac gtagcgtgga
    28921 acatcttctg gagggtcaat tctgaaatga gtaagggcat gaatcgggac ggcgacggcc
    28981 gcaacaactt gggagaaatc gagtaacaac ctgcatcgcc tgaatcagcc ttccgccttc
    29041 cgtaagttgg cgtgctgaca ccgtgccact cccttctcga agccttccgg gtagtagtag
    29101 ccggagccct gacagtccgg gcacttcttc acgtccacgc cggcaggcag gggcgatact
    29161 gcgggcgcgc tcgcctcctc agccgcaagc tggcggcggt cctttttgaa caagcgtcgt
    29221 cgcaggtgct cggccaagaa ggccggcaca cttgagacag ttgtgcgcgc ggccgcgatc
    29281 cgcaactccg tcgctaatac ctcggccacc tcgcgccatt gattcgctcc ggcacttctg
    29341 cccgtcagtt ccctttcaac ctcgcggagc gcggcgaaag cctcatcatc atcgctccgc
    29401 tcagtacttg tcttaaaaga agtcttgggc gagacggaac tatcaataga ttcaatattt
    29461 acaccctgac tagtctgact agtttctagc ctgtctagtc cgtctacttt atgggcatgg
    29521 ctagaaacac tactctgact agtcagagta gtcagagtag cggaggttct ttcctctggt
    29581 agtaaaacag tatattcatt accctcgtgt tcgccggcga tctgacgaac ctgtagcaag
    29641 ccaatggtac agaggtgtgt gatgttggag tcgaaagtga cgcgtgcgcc gatgccggct
    29701 aacttcatga gtctcggtcg ggagatacgc actgccctct taggaacaat ggcgccgcgc
    29761 gtcattgagt agagacattg gtaaagctgg ctactcttgc ccttgaattc cccggcaggg
    29821 atgacttcac gcgccacgga atttggaacc ttggtgaaat cgcgcctcgg cgaaatctca
    29881 aacgccgtat ctggctgact atcactatgc ctagtcagac tggtatgact agcaggggta
    29941 gtctgagtag tctgactagg cagggtcggc ttgatgaggt cgtcgtgtgg aaggcgtagg
    30001 gcggtaggaa ccttcaaacc tagcttatgc ttgggtttag gcacgcgcag aacgcccgct
    30061 gtcttttctt tcagttctgt catcattcac ctatttcacg gcggaaatct tgtgtcgcga
    30121 gatttgaagc cggtcaagca cctcatcggt aagcgaccag agttggtcag aggcccggtg
    30181 ggttttggcg tagagtaccg ccgggagctt ttgggagggg gcctcggcga tctttgaata
    30241 gtcgtgcaga ttggtgtcaa acacgtggcc gaccatttcg gtcgccgact caatagcagc
    30301 gcgcgactca gcagagagat tcctttgcgg tttatagaga ttaatcacag cgccgagcat
    30361 ctcaagatcg gcgttgacgt ttttgcggac ctgctcgatc gtgaaggcga ggtccgagag
    30421 gccttgcagg cccatcgcat cggcggagca gggaactagg acgaagtcgg ccgagtagag
    30481 tgcgcctgtg agcagcttgc ccagttgcgg tgggcagtct atgaagacga ggtcgtagtc
    30541 ttggccgtgc tccttcagtt ggttgcgtag ccggtgcgtc aggtaatcgg gttgcatctc
    30601 gaagcgcgcg aggcggatgt cggccggcac gaggtcaagg ctttcgaccg ggctttcgac
    30661 gatgacttcg gagaggggaa gtggttcaag cttggtgccg gcgggcgag
//
LOCUS       DASVES010000419         4600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1436611, whole genome shotgun sequence.
ACCESSION   DASVES010000419 DASVES010000000
VERSION     DASVES010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436611"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /gene="glgB"
                     /locus_tag="VF240_17220"
     CDS             <1..1237
                     /gene="glgB"
                     /locus_tag="VF240_17220"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX7177002.1"
                     /translation="IGVIMDWVPAHFPKDAWALGRFDGTALYEHLDPRLGEHPDWGTY
                     IFNYGRNEVRNFLLASALFWIDECHIDGLRVDAVASMLYLDYSRKEGAWVPNRFGGRE
                     NLEAISLLRELNEAVHRRHPGVLMIAEESTSWPLVTRPPSAGGLGFNLKWNMGWMHDT
                     LEYFKVDPLHRRHFHNKLTFGLVYAWSENFILPFSHDEVVHMKGSMLTKMFGGREWKA
                     PNLRALYGYMWAHPGKKLLFMGGEIGQWREWDNDGSIDWHLLDEPEHAGIHSLVRELN
                     RALRAQAPLYEADSEPAGFLWLDVDNALENVVAFARLAPSTGAGLVCVSNFSPVIRPG
                     YRLALPRPGAYHLLLNTDAPAFGGTGSPLAETFEAEPLPWNQQPFSALIDLPPLSTLW
                     FEAPAAPAIPPSAESQPPA"
     gene            1234..2070
                     /locus_tag="VF240_17225"
     CDS             1234..2070
                     /locus_tag="VF240_17225"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX7177003.1"
                     /translation="MNTQSDSTSEMDSTAGGLPLVIAHRGASSAAPENTLVAFHLARL
                     EGADGVEFDVRLARDGVPVCIHDATLRRTGRRDDLVSALTSAQLDEREVGDWFNLKFP
                     SAARPEYANECVPTLESTLDALRDWARAIYLEMKFERGEDFTPLAARVVEAIRARGME
                     DRVVVESFTLEAVREVGRIAPGMRKAALFKRTTGRLMPSARRIVATAVEVGAGELGLG
                     RSLVRPALLDAARRAGLKSHVWTADTPAWAERARALGLCAVITNRPALMRAAVDALRD
                     ED"
     gene            2097..2942
                     /locus_tag="VF240_17230"
     CDS             2097..2942
                     /locus_tag="VF240_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7177004.1"
                     /translation="MKRTPPQTDRIFVRPSQYTLRYSNAAHARVDLEATADFRFLFVL
                     DGELRRNAPGEAPAHTLLFLAPGDTLRASGVGRVEHVTLLLAPAFVLDCAVRARMTRD
                     DALITFRERAVSSDERLTRVARDIAEELLRDDAPGRDLLFASLVEQALVEVLRRYANI
                     QRSADVELSRAGLVDRRIRRAVELMHAHLDRDLPLEELAGAAYLSPFHFSRLFKKLTG
                     AAPHAYLASLRNARAQTLLAETDLSVTEVAERVGYSSSSHFSKAFRQATGLSPRAFRN
                     ALVRA"
     gene            3299..4510
                     /locus_tag="VF240_17235"
     CDS             3299..4510
                     /locus_tag="VF240_17235"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX7177005.1"
                     /translation="MMNRFSIRLAAALMAFLFGITASAAFIATNRVRPEDVPAPPLVD
                     SPQTLKQSGQSLEMVFVLDTTGSMGGLLEGAKQRIWGIVNEVMQTSSRPSVRIGLVAY
                     RDRGDAYVTQIVPLTDDLDRVYTTLMDFNADGGGDTPENVRRALSDGVHKAGWSRPSG
                     RVAQILFLVGDAPPHDDYQDEPAVEKTVSEAVSRGMIVNTIQCGQMPQTTPIWQQIAR
                     GGEGQYFAIAQDGGVEVVSTPYDSRLSELGSSLGRTFTAYGGGGGEKGEKHRDDAFAA
                     QAATESKVAESAAPTAAADRAINKALNSRAYVGDLLQDVENGTLKLDSVKDEDLPADL
                     KKLSADERSKEIQKRLDDRKKMRAEIVALSKQRDEHIATERRKRTGGQGGFDSAVATA
                     LKAQLARKGIK"
BASE COUNT          792 a         1717 c         1357 g          734 t
ORIGIN      
        1 catcggcgtc atcatggact gggtgcccgc gcacttcccg aaagacgcgt gggcgcttgg
       61 gcgtttcgac ggcaccgccc tctacgaaca cctcgacccg cgcctcggcg agcaccccga
      121 ctggggcacg tacatcttca actacggccg caacgaggtg cgaaacttcc tgctcgccag
      181 cgcgctcttc tggatcgacg agtgccacat cgacggcctg cgcgtagacg ccgtcgcctc
      241 gatgctctac ctcgactaca gccgcaagga gggcgcgtgg gtgcccaacc gcttcggcgg
      301 gcgcgagaac ctcgaagcca tcagcctttt gcgcgaactc aacgaggccg tccaccgccg
      361 ccatcccggg gtgttgatga tcgccgaaga gtcaacgtcg tggccgctcg tcacccgccc
      421 gccctcggcc gggggcttgg gcttcaacct caagtggaac atggggtgga tgcacgacac
      481 gctcgaatac ttcaaggtag acccgctcca ccgccggcac ttccacaaca agctcacctt
      541 cggcctcgtc tacgcctgga gcgaaaactt catcctcccc ttctcgcacg acgaggtggt
      601 gcatatgaag gggtcgatgc tgacgaagat gttcggcggg cgcgagtgga aggccccgaa
      661 cctgcgcgcc ctctacggct acatgtgggc gcaccccggc aagaagctgc tcttcatggg
      721 cggcgagatc gggcagtggc gcgagtggga caacgacgga agcatcgact ggcacctgct
      781 cgacgagccg gagcacgcgg gcatccactc gctcgtccgc gaactcaatc gcgccttgcg
      841 cgcgcaagcc ccgctttacg aagccgactc cgagccggcc ggcttcctct ggctcgacgt
      901 ggacaacgcg ctcgaaaacg tcgtcgcctt cgcgcgcctc gcgccctcga ccggcgcggg
      961 cctggtctgc gtctctaact tctcgcctgt catccgcccc ggctatcgtc tcgcactccc
     1021 ccgccccggc gcttaccatt tgctcttaaa caccgacgcc cccgccttcg gcggcacagg
     1081 ctcgccgttg gccgagacgt ttgaagcgga gccgcttccg tggaaccagc agcccttctc
     1141 cgccctcatc gacctgccgc cgctttcgac cctctggttc gaggcgcccg ccgcgccggc
     1201 gattccgccg tccgccgaat cacaacctcc cgcatgaata ctcaatcgga ttcaacctcg
     1261 gagatggatt caactgcggg cggcctcccg ctcgtcatcg cacaccgcgg cgcttcgtcc
     1321 gccgcgcccg agaacactct ggtcgccttc cacctcgccc gccttgaagg cgccgacggc
     1381 gtggagttcg acgtgcgact cgcgcgcgac ggcgtccccg tctgtatcca cgacgcgacg
     1441 ctgcgccgga cggggcgacg cgacgactta gtctccgcgc tcacgtccgc gcaactggac
     1501 gagcgcgaag tcggcgactg gtttaacctg aaattcccat cggcggcgcg acccgagtat
     1561 gcgaatgagt gcgtgccgac acttgagagc acgctcgacg cactccgcga ttgggcgcgc
     1621 gccatctatc tggagatgaa gttcgagcgg ggcgaagatt tcacgccgct cgccgcgcgt
     1681 gtggtcgagg cgatacgcgc gcgcgggatg gaggatcgtg tcgtcgttga gagcttcacg
     1741 ctcgaagccg ttagagaggt cgggcgcatc gcccccggga tgcggaaggc cgcgctcttc
     1801 aagcggacga cggggcgact gatgccttcg gcgcggcgca tcgtcgcgac ggccgtcgaa
     1861 gtcggcgccg gcgaactcgg gctgggccgc tcgctcgtcc ggcccgcgct gctcgacgcc
     1921 gcccggcgcg cgggcttgaa gtcacacgtc tggacggccg acacacccgc ctgggctgag
     1981 cgcgcccgcg cgctcggtct gtgcgccgtc atcaccaacc gcccggcgct catgcgtgcg
     2041 gcggtcgatg cgctgcgtga tgaagactga cggccggcta cgctgtcaca cacctgatga
     2101 aaagaactcc gccccagaca gacaggattt tcgtgcggcc gtcgcagtac acgctccgct
     2161 actcaaacgc ggcccacgcc cgcgtcgatc tcgaagcgac cgcggacttc cgttttctct
     2221 tcgtgctcga cggcgaactc cgccgtaacg cgccgggtga agcgcccgcg cacacgctcc
     2281 tgttcctcgc ccccggcgat acgttgcgcg cgtcgggcgt cggccgcgtc gagcacgtca
     2341 cactcctcct cgccccggcc ttcgtcctcg actgcgccgt gcgcgcccgc atgacgcgcg
     2401 acgacgccct gattactttc cgcgagcgcg ccgtctccag cgatgagcgc ctgacgcgcg
     2461 tcgcccgcga catcgccgaa gagcttctgc gcgacgacgc gcccggccgc gaccttctct
     2521 ttgcctcgct cgtcgagcag gcgctcgtcg aagtcctgcg tcgatacgcg aacatccagc
     2581 gctcggccga cgtggaactt tcgcgcgccg gcctcgttga ccggcgcatc cgccgcgccg
     2641 tcgagttgat gcacgcgcat ctcgaccgcg acctaccgct cgaagaactc gccggggccg
     2701 cgtatctctc gccgttccac ttctcccgtc tcttcaaaaa actcaccggc gccgcgcccc
     2761 acgcctacct cgcctcgctc cgcaacgcac gcgcgcagac cctcctcgcc gagaccgacc
     2821 tctccgtcac cgaagtcgcc gagcgcgtcg gctacagtag ctccagccac ttctccaaag
     2881 ccttccgaca ggccaccggc ctctcgcccc gcgccttccg caacgcgctc gtgcgggcat
     2941 agagcatcag ccagactgtc gatgccgaca gtaccgcgag cagtagcgag cgggtctggc
     3001 ctacgtgggg cgggccttta tcgcagccgc acccgctcgc taccgctcgc ggtactgttc
     3061 atgcctgagt cgcgcccagg caccccctca gttttttcgt gcaagccccg ccacgccctg
     3121 ccgttcaatc ttcggaaacg ccgcgacgga cacagcgagt ggctaaccct gccgcgcttc
     3181 cgaacgatct ttaccgcccg ccgcgtgagt tttccgccgc ccggaacttt ctccgttgac
     3241 gggtgtctat tgaaagaaac gggccgccag acacggccca ccagaaggaa aggaacttat
     3301 gatgaaccgc ttctccatcc gactcgccgc cgcgctcatg gccttcctgt ttggaattac
     3361 cgccagcgcc gccttcatcg ccaccaaccg cgtccgcccc gaagacgtgc ccgcgccgcc
     3421 gttggtcgat tcgccgcaga cattaaaaca gtccggccag tcgcttgaga tggtcttcgt
     3481 cctcgacacc accggctcga tgggcggcct gctcgaaggg gccaagcagc gaatctgggg
     3541 catcgtcaac gaggtgatgc aaacctcctc gcgcccctcc gtccgcatcg gcctcgtcgc
     3601 ttaccgcgac cgcggcgacg cctacgtcac ccaaatcgtc cccctcaccg acgacctcga
     3661 ccgcgtctac accacgctca tggacttcaa cgccgacggc ggcggcgaca cccccgagaa
     3721 cgtccgccgc gcgctctccg acggcgtgca caaggccggc tggtcacgcc cctccgggcg
     3781 cgtcgcgcaa atcctcttcc tcgtcggcga cgcccctccg cacgacgact atcaggacga
     3841 acccgccgtc gagaagaccg tctccgaggc cgtcagtcgc ggcatgatcg tcaacaccat
     3901 ccagtgcgga cagatgcccc agaccacacc catctggcaa cagattgcgc gcggcggcga
     3961 gggtcaatac ttcgccatcg cgcaggacgg cggcgtcgaa gtggtctcca cgccctacga
     4021 ttcgcgtctc tccgaactcg gcagcagcct cggcaggact ttcacggcct acggcggcgg
     4081 cgggggcgag aaaggcgaga agcaccgtga cgacgccttt gcggcgcagg ccgcaactga
     4141 gtcgaaggtc gccgagagcg ccgccccgac ggccgccgcc gaccgcgcca tcaacaaggc
     4201 tctgaactcg cgtgcctacg tcggcgacct tctgcaagat gtcgagaacg gcaccctgaa
     4261 gctcgattcc gtcaaggacg aagacctccc ggctgacctc aaaaaactgt cagccgacga
     4321 gcggagcaag gagattcaga agcgcctcga cgaccgcaag aaaatgcgcg ccgagatcgt
     4381 cgccctctcc aagcagcgcg acgaacacat cgccaccgag cgccgcaagc gcaccggcgg
     4441 ccagggcgga ttcgactccg ccgtcgccac cgccctcaaa gcccaactcg cacgcaaagg
     4501 catcaagtaa ggcaagtaag cagtaagcag tgagcagtga gcagtgagca gtaagcagtg
     4561 agcagtcaac gccagtagcg cgtcctgact gctcaccgct
//
LOCUS       DASVES010000420         7659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_855532, whole genome shotgun sequence.
ACCESSION   DASVES010000420 DASVES010000000
VERSION     DASVES010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855532"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..569)
                     /gene="rpsL"
                     /locus_tag="VF240_17240"
     CDS             complement(162..569)
                     /gene="rpsL"
                     /locus_tag="VF240_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX7177006.1"
                     /translation="MPTINQLVRKGRKAVKYKTASPALQSSPQKRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNSIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGTL
                     DSVGVADRKQGRSKYGAKRPKGGAAAPAGKGKK"
     gene            1182..1838
                     /locus_tag="VF240_17245"
     CDS             1182..1838
                     /locus_tag="VF240_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177007.1"
                     /translation="MAQQGRQNVLDNYVTVAERVAAFYQTYPQGRINTQIIQHDTETG
                     FILMRAEIYRSTDDASPSATGHAFEVRSESYVNKTSYIENAETSSVGRALALLGFEVR
                     RGIASREEMDKTARMQPPERAPVERAPAEKPSPSADKAATRAAAPKAEEEPPADINAE
                     ILQAAAVLGYDAAKVRKWVNQRFEVTGGLESLTNHDKSEILQVFREKAQSAQTKAAQR
                     "
     gene            1860..2150
                     /locus_tag="VF240_17250"
     CDS             1860..2150
                     /locus_tag="VF240_17250"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7177008.1"
                     /translation="MKIEEAIKAKRGEIRRIAERHGASNVRVFGSVARGEARPESDVD
                     LLVDVTSQTSSWFPAGLILDLERLLGRRVEVVTEKALNKDIREKVLREAIQL"
     gene            complement(2435..2857)
                     /locus_tag="VF240_17255"
     CDS             complement(2435..2857)
                     /locus_tag="VF240_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177009.1"
                     /translation="MSNPPGPPVPMEEGVNQWLRAHLATIEQRIRKQAETLAKAEGRD
                     DIEPKDIAQAAQLYAPGELFTAPPRERLTLKEKVLSSISGITLISALLAIAFGIIGYA
                     AAAKGSEITSGAWDIAKIFAGAVVGSTGAAVSTAVKRD"
     gene            complement(3198..4070)
                     /locus_tag="VF240_17260"
     CDS             complement(3198..4070)
                     /locus_tag="VF240_17260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7177010.1"
                     /translation="MSIFNQLFPIASTRKFFVLLTLALLLANAYVVTAQNTANGTNSN
                     TGNSNINANLNSNANVNTNANGNQNGNANGNVSGNTNRRGGEPSATPDPKTEEIKVLI
                     AKTIWFPLAVTAIFAAVLIPFSMVIVRAIRFSRSTFNSPLGLPEGSLRAMLAFMLVAL
                     LGFYVYASVLSFSDFKPPEFLIGIVATVIGFYFGSRTSEDRGATTPRTGTVQGDVTDK
                     TGTSAGGANVELSQPDGKKLTQKADAKGKYKFDNVPAGDYDIQASLQGHAPSDPTRVK
                     VTAGAPLTVDLKLK"
     gene            complement(4752..>7659)
                     /gene="rpoC"
                     /locus_tag="VF240_17265"
     CDS             complement(4752..>7659)
                     /gene="rpoC"
                     /locus_tag="VF240_17265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7177011.1"
                     /translation="KAIKIHPLVCTAFNADFDGDQMAVHIPLSPEAQIEAAVLMLASN
                     NILSPANGQPIAVPSQDIVLGCYYLTKDKAGAKGEGRIFSSTDEVLLALDAHEVETQT
                     PIKLRVRGELIDLTQEHDTQDVMRAAVQENVSRVITTTVGRVIFNDQIPKGLAYFNGT
                     LKKKGLQSLVNFCHLRLGHETTVKMLDDLKSLGFLYATKAGISIGIDDLVTPDAKKEL
                     VSKADRDVIEVEKQYRDGVITNGERYNKVIAIWSEVTDKVAKEMFLAMSAKEKATGEQ
                     NPIMIMSDSGARGTAQQIRQLAGMRGLMAKPSGEIIETPIRSNFREGLNVLQYFISTH
                     GARKGLADTALKTADSGYLTRRLVDVAQDVIISEPDCGTLKGITTTAIIEGGEEIESL
                     RDRIVGRTALEDVVDPVEGAVLVEANDEINEDLSVEIQRSGVQRVRIRSVLTCESRRG
                     VCIKCYGRNLATGKPVDIGEAVGVIAAQSIGEPGTQLTMRTFHIGGTARLEESSKHEA
                     RMDGTIKYVELNVVKNRRGEIIAMNRNGALTIIDERGREVARHQIVYGAHIHAEDGAR
                     VKEDQVLATWDPFTFAILTEVAGQIKYQDLKEGVTVDEEVDEVTGQSRVVVKDSPDEK
                     KQPRLEIRSASNKVLKTYQMPVRANLMVHDGDQAEPGDVIAKIPRETTKTKDITGGLP
                     RVVELFEARRPRETAIMSEIEGMVSLGPIAKGKRKIIVTGDDGEQREYDIPRGTHINV
                     QEGDRVRAGEALMDGPLNPHDILRVLGMNRLQEYLTNEIQEVYRLQGVNINDKHIEVI
                     VRQMLRWVKISDVGDTDFLLDEQVDRFRFSDENARVTADGNGEPAKAEPLLLGITKAS
                     LSTDSFISAASFQETTRVLTEAAISGRIDYLRGLKENVIMGRLIPAGTGMEYYRNVRV
                     DRDPTVDQAREEPSIDIADLLPAPPEVPTPTTVKAVAAGDGDAEVETEADVDDE"
BASE COUNT         1373 a         2294 c         2411 g         1581 t
ORIGIN      
        1 tcacgacgat ctgcaactct tcttctaggc atgatcagct ctcagctttt agcagttagc
       61 tgttagcgaa aaactaacag cgccaacgct ctcatttaat ccttaagccg tccgttacga
      121 attcaaagct aatagctaat agctaatagc taacggctcc cttacttctt gcccttgccg
      181 gccggtgcgg cggcgccgcc cttcggccgc ttggcgccgt acttggagcg cccctgcttg
      241 cggtcggcga cgcccaccga gtcgagcgtg ccgcggatga cgtggtagcg cacgccgggc
      301 aagtctttga cgcggccgcc gcggatgagc acgatggagt gctcctgaag gttgtggccg
      361 acgccgggga tgtaggtcgt cacctcgatg ctattggtca ggcgcacgcg cgccaccttg
      421 cgaagcgccg agttcggctt cttgggcgtc tgcgtgtaga cgcgcgtgca gacgccgcgc
      481 ttctgcgggc tggactgaag cgccgggctg gcagtcttgt acttcaccgc tttgcggccc
      541 ttacgcacga gctggttgat ggtcggcact ggtcttctct tccttccgtt cggagccggt
      601 gacctgtccg caagcgtaaa ggcggcctgt tcctgcctct taccggaaaa tttccggcag
      661 tcagcgcaag acgggctttc gggcctcgca agcggttgat gttgaatttg agggacgttc
      721 tggctgcgtc ctaacggacg ggcgtggatg ctggcctccc ccgaagccga aacttttctg
      781 tggcgtcgca aattgctgga ggcgagccgt aaggctcggt tctcgcgccc ccgtcgccgg
      841 gccgcgttct ccgtaaggaa ttgcgcgggg cggcggggct ttacgccaca atatttgtca
      901 aacggccgta agcccccacg ggcacgaccg ggcaacgcct ccacaccggc caaaaccttc
      961 taagcgttgc tctatgggga aatggactgc aagtccaaac gggagactat acgggcgacg
     1021 ccccatcctg tcaagcgcgc cccgtagcct cacggccgac agacgccggc gcggccgttt
     1081 tctcggcccc gtgtttctgc tacagtttac ccgcgaacgc gattttgatg cggcgcggcc
     1141 tcttttcgaa gccgccgcgc gggcaaaaaa ggggtgaaaa aatggcgcag cagggcagac
     1201 agaatgtttt ggacaactac gtgaccgtgg ccgagcgggt ggccgcgttc tatcagactt
     1261 acccacaggg acgcatcaac acacagatca tccaacacga caccgagacg ggcttcatcc
     1321 tcatgcgcgc cgaaatctat cgctccacgg acgatgccag cccctcggcg acggggcacg
     1381 ccttcgaggt gcgctcggag tcttacgtga acaaaacttc ttacattgag aacgcggaga
     1441 cgagttccgt gggccgggcg ctggccctgc tcggcttcga ggtgcgccgc ggcatcgcca
     1501 gccgcgagga gatggacaag accgcacgca tgcagccccc cgaacgcgcg cccgtcgaaa
     1561 gagcgccggc cgagaagcct tcaccctccg ctgacaaggc ggccacgagg gccgccgcgc
     1621 ccaaggccga ggaggagccg cccgccgaca tcaacgccga aattcttcaa gccgccgccg
     1681 tcctcggcta cgacgccgcc aaggttcgca agtgggtcaa ccaacgcttc gaggtcaccg
     1741 gcggcctcga aagcctcacc aaccacgaca agagcgagat actgcaagtc ttccgcgaga
     1801 aggcccagag cgcacaaacg aaagcggcgc aaaggtagct tgctgaagca tgtgtgatta
     1861 tgaaaatcga ggaagccatt aaagcgaagc gcggcgaaat ccgccgcatc gccgaacgac
     1921 acggcgcgtc gaacgtgcgc gtgttcggct ccgtcgcgcg cggcgaggca agacccgaga
     1981 gtgacgttga tttgctcgtt gacgtgacct cacaaacaag ctcatggttt ccggcggggc
     2041 tgatcctcga tttggaaaga ctgctggggc ggcgagtcga agtcgttacg gagaaggcgc
     2101 ttaacaaaga cattcgcgag aaggtgttgc gcgaagccat acagctatga aagatgacgc
     2161 ggtttacctg cgccatgtcg cggaaaggtg gatggctacc taatccaatc cctgcattgg
     2221 ccgcctaatc cccagaccaa cacagatttc ttagcaaaca tgccaggccc aggagattag
     2281 gcggctaaat gggcgtaatc catgacggcg attagtacgc ataatttaaa ttagattgtt
     2341 ccctctggtg acaacatccc acaagtaaaa cgaagccccc accggcgcga caaagaaaag
     2401 gctcaggccg caaaacggcg ggttggcggc gggatcaatc acgtttgacc gcagtagata
     2461 cggctgcccc ggtagagccg accaccgcgc ccgcgaatat cttggcgatg tcccaggcac
     2521 cggaggtgat ctcactgccc tttgccgccg cggcgtagcc gatgatgccg aaagcaatcg
     2581 ccaagagcgc ggatattagt gtgatacctg agatcgatga caacaccttc tctttgaggg
     2641 taagtctctc tctcggcggg gctgtgaaca gttcgcccgg tgcgtacagt tgcgccgcct
     2701 gcgctatgtc cttaggttca atgtcatcgc gtccctcagc cttcgcgaga gtttcagcct
     2761 gtttcctgat tctctgctcg atggtggcga ggtgggcgcg gagccattgg ttcacgcctt
     2821 cttccatggg cacgggcggg ccgggaggat tactcataag tagaccttct tccgttttaa
     2881 gttttgacgt gaggccggcg tagctctcgc cggcgggaag ccttcttgag cgcgggcaac
     2941 atattccaac tctggatggg tgtcaataat aaaggggcaa ggctctcgcc ccgccccctt
     3001 ctgccatccg gccgcgtcgc cagatacgga aggggcgggg ttaaggctcc gccactcttc
     3061 atcatcagcg cccaagacgg agcgcatcgg gagcgacgcc ctcagaagca agcagcggtt
     3121 cgcgctggag cgccagagga ctaacgccga ggccgcgcgc ggcgcgaggc acttcgctta
     3181 gccttacaca gtgcgagtta cttcaacttc agatcgacgg tcagcggcgc gcccgccgtc
     3241 accttcaccc tcgtcgggtc acttggcgcg tggccttgta acgaagcttg aatatcgtaa
     3301 tcgcctgccg gaacgttatc gaatttatac ttgcccttgg cgtcggcttt ctgcgtaagt
     3361 ttcttgccgt ctggttggga caactcaaca ttcgctcctc cggctgaagt cccagtcttg
     3421 tccgttacat ccccctgtac ggtgccagtc cgtggagttg tggcgcctct gtcttcgctg
     3481 gtgcgcgagc cgaaatagaa gccgatgacg gttgcgacga taccgatcag gaactccggt
     3541 ggcttgaaat cggagaaact caggacgctt gcataaacgt agaagcccaa caacgcaacg
     3601 agcatgaagg cgagcatcgc tctcagggaa ccctctggta acccgagcgg gctattaaac
     3661 gtcgagcgcg agaatctaat ggctcgcacg atgaccatac tgaatggaat caacacggcg
     3721 gcgaatatcg cggtgacggc gaggggaaac cagatagttt tggctatcaa aactttgatc
     3781 tcctctgtct ttggatctgg agtcgctgac ggttcgcccc ccctccggtt agtattgccg
     3841 ctaacattgc cattcgcgtt gccattctgg ttgccgttgg catttgtatt cacgttcgcg
     3901 ttactgttga gattagcgtt aatgttcgag ttaccggtat tgctgttcgt accgttggcg
     3961 gtgttttgtg cggttacgac atacgcattg gcaagcaata gagcaagcgt aagcagcacg
     4021 aagaatttgc gtgttgaagc gatggggaaa agctggttga agatagacat gcgtttgctc
     4081 ctttcgcatt tcatgaccgg gttgaatgag ttgttgggag gtggtgtcca ctagatgtgc
     4141 caccgccgcg gaagactcaa atcacgactc tcagttaatc tccccaaccg cgcctgcgcc
     4201 cgtctgggag ttgtagtaaa gaatcctttt cgttgtgttg acgatgtgcg tccaccctgt
     4261 tgccggccca ctgttctcta aatgtttatg ccatttcggg gcaaggcatt tcatcgggtc
     4321 gcccgtatta aagtcgtggc ctacggctgc ggcgcctacg ccgccatcct cgacgccgac
     4381 gaaattagtc tgttgaaatg agataggggc gaagcctccg cccctatccg tccacgtgga
     4441 cggatagatc aacccttccc gccgcgcgct gacacacgcc gaacttcacc cttccgtcgg
     4501 agcgagaggg gcgtgtgatg tggaccttac tgaatcgggg gtggcctcac ggcctcccgt
     4561 ctgcgcgccg ctgatgcctg gctaattcgg gcgttatgct gtcgtcttac atttgtgagt
     4621 gtgcagagaa aacctgactc aataccctga cgaatcttca gagaaagtat tgggcgggaa
     4681 agttaaaggg ccggcggggg tttctaaacc cccgccgacc cttttctact tcggacagtt
     4741 gaaggcgaag cttactcgtc gtccacgtcg gcttccgttt ccacctctgc gtcgccgtcg
     4801 ccggcggcca cggccttgac ggtcgtgggc gtggggactt cgggcggcgc ggggaggagg
     4861 tcggcgatgt cgatggaagg ctcctcgcgg gcctgatcga cggtcgggtc gcggtcaacg
     4921 cggacgttgc ggtagtactc catgccggtg ccggccggga tgagccgccc catgatgacg
     4981 ttctccttga ggccgcgcag gtagtcgatg cggccggaga tggccgcctc cgtgagcacg
     5041 cgtgtggtct cctggaagga ggccgcggag atgaaggagt ccgtcgagag cgacgccttc
     5101 gtgatgccca gcaggagcgg ctcggccttg gccggctcgc cgttgccgtc ggccgtgacg
     5161 cgggcgttct cgtcggagaa gcggaagcgg tcaacctgct cgtcgaggag gaagtccgtg
     5221 tcgcccacgt cggagatttt cacccagcgg agcatctggc ggacgatgac ctcgatgtgc
     5281 ttgtcgttga tgttgacgcc ctgaagacga tagacctcct ggatttcgtt cgtcaggtac
     5341 tcctgaaggc ggttcatgcc gagcacgcgc aggatgtcgt gcgggttgag cgggccgtcc
     5401 atcagcgcct cgccggcgcg gacgcggtcg ccctcctgca cgttgatgtg cgtgccgcgc
     5461 gggatgtcgt actcgcgctg ctcgccgtcg tcgcccgtga cgatgatctt gcgcttgccc
     5521 ttcgcgatgg ggccgagcga gaccatgcct tcgatctccg acatgatggc cgtttcgcgg
     5581 ggccgacgcg cctcgaagag ttcgaccacg cgcggcaggc cgcccgtgat gtctttggtc
     5641 ttcgtcgtct cgcgcggaat cttcgcgatc acgtcgcccg gctcggcctg atcgccgtcg
     5701 tgcaccatga ggttggcgcg caccggcatc tggtaggtct tgagaacctt gttcgacgcg
     5761 ctgcgaattt ccaagcgggg ctgcttcttc tcgtcgggcg agtccttgac gacgacgcgc
     5821 gactgtcccg tcacctcatc cacttcctcg tcaaccgtga cgccttcctt caagtcctga
     5881 tacttgatct ggccggcgac ctcggtgagg atggcgaagg tgaacgggtc ccatgtggcg
     5941 agcacctgat cttccttgac gcgcgctccg tcctcggcgt gaatgtgcgc gccgtagacg
     6001 atctggtggc gcgccacctc gcggccgcgc tcgtcgatga tggtcagggc gccgttgcgg
     6061 ttcatcgcga tgatttcgcc gcggcggttc ttgacgacgt tcagctcgac gtacttgatc
     6121 gtgccgtcca tgcgagcctc gtgcttcgag gactcttcga ggcgggccgt gccgccgatg
     6181 tggaacgtgc gcatcgtgag ctgcgtgccc ggctcgccga tactctgcgc ggcgatgacg
     6241 ccgacggcct cgccgatgtc caccggcttg cccgtggcga ggttgcggcc gtagcacttg
     6301 atgcacacgc cgcggcgcga ctcgcacgtc agcaccgagc ggatgcggac gcgctggacg
     6361 ccggagcgtt gaatctcgac ggagaggtct tcgttgatct cgtcgttggc ctcgacgagg
     6421 acggcgccct cgaccgggtc aaccacgtct tcgagcgccg tgcggcccac gatgcggtcg
     6481 cgcaaagact cgatctcctc gccgccctcg atgatggcgg tcgtggtgat gcctttgagc
     6541 gtgccgcagt caggctccga gatgatcacg tcctgcgcca cgtccaccag acggcgcgtg
     6601 agatagcccg agtcggcggt cttcaaggcc gtgtcggcga ggcccttgcg cgcgccgtgc
     6661 gtcgagatga agtactggag gacgttcaag ccctcgcgga agttcgagcg gatgggcgtc
     6721 tcgatgattt cgccggaggg cttggccatg aggccgcgca tgccggcgag ctgccgaatc
     6781 tgctgcgcgg tgccgcgcgc gccggagtcc gacatgatca tgatcgggtt ctgctcgccc
     6841 gtcgccttct ccttcgcgct catcgcgagg aacatctcct tggcgacctt gtcggtcacc
     6901 tccgaccaga tggcgatgac cttgttgtag cgctcgccgt tggtgatgac gccgtcgcgg
     6961 tactgcttct cgacctcgat cacgtcgcgg tcggccttcg agaccaactc tttcttggcg
     7021 tcgggcgtga cgaggtcgtc gatgccgatg gagataccgg ccttcgtggc gtagaggaat
     7081 ccgagactct tgaggtcgtc gagcatcttg acggtcgtct cgtggccgag gcgaaggtgg
     7141 cagaagttga cgagcgactg gagacccttc ttcttgaggg tgccgttgaa gtaggccaga
     7201 cccttcggga tttggtcgtt gaagatgacg cggcccaccg tcgtggtgat gacgcgcgag
     7261 acgttctcct gcacggcggc gcgcatcacg tcctgcgtgt cgtgctcctg cgtcaggtcg
     7321 atgagttcgc cgcggacgcg cagcttgatg ggcgtctggg tctccacctc gtgcgcgtcg
     7381 agggcgagca gcacctcgtc ggtcgagctg aagatgcggc cctcgccctt cgcgccggcc
     7441 ttgtcctttg ttaggtaata gcagccgagg acgatgtcct gcgagggcac ggcgatgggc
     7501 tggccgttgg ccgggctgag gatgttgttg gaggcgagca tgagcacggc cgcctcgatc
     7561 tgcgcctcgg gcgagagcgg gatgtggacg gccatctggt ccccgtcgaa gtcggcgttg
     7621 aaggccgtgc agacgagcgg gtgaatcttg atggccttg
//
LOCUS       DASVES010000421        11906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_469758, whole genome shotgun sequence.
ACCESSION   DASVES010000421 DASVES010000000
VERSION     DASVES010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469758"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VF240_17270"
     CDS             <1..975
                     /locus_tag="VF240_17270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX7177012.1"
                     /translation="QEVRWCPGCGDYAILNNIQKVMPELGIPREKIVFVSGIGCSSRF
                     PYYMNTYGFHSIHGRAPAVATGIKASNPDLSVWVITGDGDGLSIGGNHFMHAIRRNLD
                     INIILFNNRIYGLTKGQYSPTSEFGKKTKSTPMGVIDYPVNPLSLAIASEATFVARAI
                     DIDVKHLGAMVQEAARHKGVSFVEVFQNCNIFNDGAFDSFAPSNVRSENVLYLEHGKP
                     LVYGKARDKGIRMSGTLPEVIQLGSETGLTEDDCLVHDAHVEDPSVAFMLARMEQPNY
                     PQPVGIFRDVQRESYESLMAGQIEAAVAKQGRGSLNKLINSGDMWVVE"
     gene            complement(1064..1384)
                     /locus_tag="VF240_17275"
     CDS             complement(1064..1384)
                     /locus_tag="VF240_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177013.1"
                     /translation="MVSRKTQSKAGIAEVRRGQQMGDERSQPSKVDEAKRATPALRGR
                     RKTESKFYADESSQSVGSDGEAPRDNSPSVPAAIPTGTRLGESGGERAFKQRQSRASK
                     KRPG"
     gene            complement(1528..4128)
                     /gene="lon"
                     /locus_tag="VF240_17280"
     CDS             complement(1528..4128)
                     /gene="lon"
                     /locus_tag="VF240_17280"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7177014.1"
                     /translation="MPEDTFDDVERAVEMSGKSADTPPPPKVARGRGAPPSADAEPKP
                     EPEQSAPAKGQSSGGEPGVFEIPVLPLQNTTLFPGTIVPLAAGRPRSVSAVEAALATE
                     EKLVVCVTVRDGRALEAEAVPPDDLYTVGTLVTIKRMMRAEEAVQIIVQGVDRVKVLQ
                     WSQTDPHLRARVHVLPVPTKRDDEHVEALFRNVQQLIQRALALLPEIPPEVRAAVMNT
                     SDPVQIAYFLGSLLNLGVEQEQQMLEADTADELLQIAHSRLAREIEILELRSRISNEA
                     QGEMQKAQRDYFLRQQMKAIQKELGEDEGGEAAEAAMMRERLDQADLPEDVRKEAERE
                     LRRMERLPQAAPDYHVIRSYLEYILELPWKKSSEDKLDLNEARRILDEDHYGLEDVKE
                     RILEFLAVVKLRPDSKSPILCFVGPPGVGKTSLGMSIARALGRQFERLSLGGVRDEAE
                     LRGHRRTYIGAMPGRIIQSLRRAAVNNPLMMLDEIDKLGADFRGDPASALLEILDPQQ
                     NHTFRDHYIDLPFDLSRVFFIATANSLGPIPMPLRDRMEIIYLAGYSDQEKLHIARRY
                     LIPRQTKENGLAPEQIEITDDAVNLMAARYTREAGVRQLERTVGSVARKVALKVAQGD
                     AERVIVDAADLKELLGPPRFYPEEARKEVPVGVATGMAWTEMGGQLLFIEATLLPGSS
                     GLTLTGQLGEVMQESARAARSYLWSHAAEFGIDPQMFKSYGFHLHVPAGAIPKDGPSA
                     GVAITSAMASLTTGRRVRPDTAMTGEITLSGLVFPVGGIKEKILAAHRAGLRRVVMPE
                     RNEADTEDIPEDVRKELELVFVKTTAEAVEATLEKLVATPPPPAAPATDEGDRASKQP
                     APEAVRVRER"
     gene            complement(4166..4540)
                     /locus_tag="VF240_17285"
     CDS             complement(4166..4540)
                     /locus_tag="VF240_17285"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7177015.1"
                     /translation="MARSIEGYLRMLPKADGMLPAGRLWAPPADVYRTRDGWIVKLEL
                     AGIRADELEVEISGAQLSVRGCRRDTVCTEGMVCQQIEITYSRFEKTIRFPCPIEGAA
                     VERDYRDGLLILYLKSSEHCEE"
     gene            complement(4638..5612)
                     /locus_tag="VF240_17290"
     CDS             complement(4638..5612)
                     /locus_tag="VF240_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7177016.1"
                     /translation="MSRYRDDRDDYYNSERGRSERRREEFYDDDRQAAPPTRGGGYER
                     GGGSEDYGGGGRERERYYERPPSDYGRADYEPRYGRGDYDEERTVVRRTAGSGAIARR
                     RYEEDDSGEYERGGERGGGRSILDEGRDFFRRTFGGRPARRPGGPSRSHVRCRDIMTR
                     DVTVATRETTLEGVARMMRDEDTGVIPVIDRAANLTDLAETDRPDINRVEANVVGSSG
                     KLVGLITDRDIVVRALAEGKDPRTVRAEEIMTTDIHTAHPNDRVVEAVRKMGDKQVRR
                     IPILDRDGTLRGIISMADVALETEDDHELAEALEEISSGSTFWNRVFG"
     gene            complement(5926..6948)
                     /locus_tag="VF240_17295"
     CDS             complement(5926..6948)
                     /locus_tag="VF240_17295"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7177017.1"
                     /translation="MLKNKFFTAAAALAICCVFAAQAAAASKEITVRGQLRPTVESGG
                     WVIVAGEQKYLLINADRFKGESWFRKDAEVEAVGETKNAITIYMEGTPFEARTLRPTG
                     KRAEESAAASGNGALTGGAAAAVAELTRITVSGEATVQAQPDTAIVSIAVVTQNASAS
                     EAQAENASRTEATVRAVKAAAGAGAEVKTGGYSLQPQYSYKPNESPQINGYQVRNSVI
                     VTMSDLTKVGAVIDAASRAGANTVDYLNFTLRRDRPARSQALAEATRDARAKAQTLAE
                     ALGGRVVRIVEVQEGPLYRPPVPLARGTVGRMAEMQASADTPVEVGSLDIRAQVQLVA
                     EVEMRP"
     gene            7128..7484
                     /locus_tag="VF240_17300"
     CDS             7128..7484
                     /locus_tag="VF240_17300"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7177018.1"
                     /translation="MQTLGPKFPNSRRSARTGELVTVGRVEDVPEGRGATVELKGGRE
                     LALYNVCGRFHAVENFCPHKGAPLADGALCGPTVTCDWHGWRFDVTTGACLNRPGESI
                     EAYEVVIEDGWIKIRV"
     gene            complement(7591..7821)
                     /locus_tag="VF240_17305"
     CDS             complement(7591..7821)
                     /locus_tag="VF240_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX7177019.1"
                     /translation="MADPNDRVPENASGTFYVDMQCIDCDVCRDTAPDNFTRADSNGY
                     SFVYKQPTSADERALCEEALLACPVEAIGSDG"
     gene            complement(7908..9026)
                     /gene="nadA"
                     /locus_tag="VF240_17310"
     CDS             complement(7908..9026)
                     /gene="nadA"
                     /locus_tag="VF240_17310"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX7177020.1"
                     /translation="MTVLTETRPEAPALEDYFGLSDAELRERIETVRRQLGRRLVILG
                     HHYQRDDVIRHADYTGDSFKLSQLTSKRPDAEFIVFCGVHFMAESADILKPGDQKVIL
                     PDLGAGCSMADMATAEQVEDAWDQLEALGIIGEGSRVVPVTYMNSSAAIKAFCGEHEG
                     VVCTSSNAVPLFEKYLQEADKLFFFPDQHLGRNTGHRFGIPADEMVLWDPHQELGGNT
                     EEQLRRARIILWKGHCSVHGRFRPWHVEQVRRETPGIKVLVHPECTREVVEMSDLSGS
                     TEFIIKTIREAPAGSRWAVGTEVNLVNRLVKQFPDKEIRLLAPDLCMCATMYRIAPQN
                     LAWALESLLAGHVVNQITVPEETAAWARVALDRMLQIK"
     gene            9611..10582
                     /locus_tag="VF240_17315"
     CDS             9611..10582
                     /locus_tag="VF240_17315"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7177021.1"
                     /translation="MKNNQRARLSRVSRLAVFALLGVTLVAGAAVWGVPGASANHPVL
                     VEGNCDSPVPGTNLVEKGTCGDFDGDGRIGTAEDTDGADRIFGTINAALGPGTGAAAG
                     TGANHNGRITIVKSGRFAEQIYIGQNLQGAGGPGVAIPGNVTIEAAPGVEANIDAILQ
                     GDPAGGNVARTAGTGIQIVYTNASRVVTLRNLIIRNFANGIQTFLDSRVHIDNCRLEN
                     NLNYGIQVTTNSRVVISNSRIDGTGYRTGIGSSVPVPGTGILVENNAKALIENTTISH
                     SAGTGIDNTTGFTANVVLFKVGSYFNTSNIGNNGALVAPNPGFATID"
     gene            complement(10713..10898)
                     /locus_tag="VF240_17320"
     CDS             complement(10713..10898)
                     /locus_tag="VF240_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177022.1"
                     /translation="MAGLFEQRQTRRRRLAKLSFEEKIEIVERLRDLRLNRSFMRTKE
                     TGEQFDEYVRPEEMTRP"
     gene            complement(10913..11422)
                     /locus_tag="VF240_17325"
     CDS             complement(10913..11422)
                     /locus_tag="VF240_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177023.1"
                     /translation="MEKTLQVLNRMEAEGVVGRYAIGGAIAATFYVEPVSTFDVDVFF
                     AAEGASDALLSLTPLYDYLAALGYEPAGEAVEIEGWPVQFLPVFTPLLAEALTQAVEI
                     RFKKTPTRVMRAEHLVAVMLETGRAKDLARVIQFLEEGAVELSALEGVLARHGLAERW
                     REFRNRFDV"
     gene            complement(11460..>11906)
                     /locus_tag="VF240_17330"
     CDS             complement(11460..>11906)
                     /locus_tag="VF240_17330"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX7177024.1"
                     /translation="EPGSSIMPGKVNPTQSEALTMVAVQVLGNDAAIGFAGSQGNFEL
                     NVFNPVMIHNLLHSIRLLRDACRSFVHHCVEGITLNRERIEENLRNSLMLVTALNPHI
                     GYDNAAKLAKNAHRRGISLRESAVELGLLTGEQFDEYVKPEEMTHP"
BASE COUNT         2020 a         3876 c         3859 g         2151 t
ORIGIN      
        1 caggaggtca ggtggtgtcc gggctgcggc gactacgcca tcctcaacaa catccagaag
       61 gtgatgcccg aactcggcat cccccgcgag aagatcgtct tcgtcagcgg catcggctgc
      121 tcctcgcgct tcccctacta catgaacacc tacggcttcc actccatcca cggccgcgcg
      181 ccggcggtgg ccaccggcat caaggcctcg aacccggact tgagcgtctg ggtcatcacc
      241 ggcgacggcg acggactctc aatcggcggc aaccacttca tgcacgccat ccgccgcaac
      301 ctcgatatca acatcatcct cttcaacaac cgcatctacg gactcaccaa gggccagtac
      361 tcgcccacct cggagttcgg caagaagacg aaatccaccc cgatgggcgt catcgactac
      421 cccgtcaacc ccctgtcgct cgccatcgca tctgaagcga ccttcgtcgc ccgcgccatc
      481 gacatcgacg tgaagcacct cggcgcgatg gtgcaggagg cggcccgcca caagggcgtc
      541 agcttcgtcg aggtcttcca gaactgcaac atcttcaacg acggcgcgtt cgactccttc
      601 gccccgagca acgtgcgctc ggagaacgtg ctctatcttg agcacggcaa gccgctggtc
      661 tacggcaagg cgcgagacaa gggcatccgc atgtcgggca cgctcccgga agtgatccaa
      721 ctgggtagtg agaccggact caccgaagac gactgtctcg tccacgacgc gcacgtcgaa
      781 gacccctcgg tcgccttcat gctcgcgcgc atggagcagc ccaactaccc gcagccggtc
      841 ggcatcttcc gcgacgtgca gcgcgagtcc tacgaatcgc tgatggccgg tcagatcgag
      901 gccgccgtcg ccaagcaggg ccgcggcagt ctcaataagc tcatcaacag cggcgacatg
      961 tgggtcgtcg aataaggcga aagtaaaaag gcaaaaggca aaagtggaaa ggcatcacgc
     1021 ctcttcactt ttgccttttg cctttttact ttcgccttga cgctcagccg gggcgttttt
     1081 tagacgcgcg ggattgtctc tgcttgaagg cgcgctcgcc gccggactcg ccaaggcggg
     1141 tgccggtggg gatggcggcc gggacggagg ggctgttgtc gcgcggggcc tcgccgtcgc
     1201 tgccgaccga ttgcgaggac tcgtcggcgt agaacttgct ctcggtcttg cggcgaccgc
     1261 gcagggccgg cgttgcgcgc ttggcctcgt ccaccttcga cggctggctg cgctcatcgc
     1321 ccatctgctg cccgcgtcgc acctcggcga tgcccgcctt gctctgtgtc ttcctgctca
     1381 ccattttccg cacctttcaa aagccgtaaa tggtaaatgg taaatcgtga ttaacgctcc
     1441 cgccgcttga gataaacgcc ggaagctgaa cgatgctcaa cctcgattta cgatttacga
     1501 ttcacgattt acgattcacg gttcacttca tctttcgcga acacgcacgg cctcgggggc
     1561 gggctgcttc gaggcccggt cgccttcatc ggtcgcgggc gccgccggcg gtggcggagt
     1621 ggcgacgagt ttctctaacg tcgcttccac ggcctcggcc gtcgtcttga cgaagaccag
     1681 ctccagctcc ttgcgcacgt cctcagggat gtcctcggtg tcggcctcgt tgcgctcggg
     1741 catgacgact ctccgcaacc ccgcgcggtg cgcggccagg attttctcct tgatgccgcc
     1801 gacggggaag accagacccg agagtgtgat ctcgccggtc atcgccgtgt cggggcgcac
     1861 acgccgaccc gtagtgagcg aggccatggc cgacgtgatg gcgacgccgg ccgaggggcc
     1921 gtccttcggg atggccccgg cgggcacgtg caggtggaag ccgtaggact tgaacatctg
     1981 cgggtcgatg ccgaactcgg cggcgtgcga ccacaggtaa gagcgcgccg cgcgcgccga
     2041 ctcctgcatc acctcgccga gctgacccgt cagtgtcagg ccggagcttc cgggcaacag
     2101 cgtcgcctcg atgaagagga gctgtccgcc catctccgtc caggccatcc ccgtcgcaac
     2161 gccgacggga acctccttgc gcgcctcctc ggggtagaag cgcggcgggc caagcaactc
     2221 cttgaggtcg gcggcgtcaa cgatgactcg ctccgcgtcg ccctgcgcga ccttcagggc
     2281 caccttgcgc gcgaccgagc cgacggtgcg ctccagttgc cgcaccccgg cctcgcgcgt
     2341 gtagcgcgcg gccatcaggt tgacggcgtc gtccgtgatc tcgatctgtt cgggcgcgag
     2401 gccgttctct tttgtctgtc gcgggatgag atagcggcgc gcgatgtgga gcttctcctg
     2461 atcgctgtag ccggcgaggt agatgatctc catgcggtcg cgcaggggca tggggatggg
     2521 gccgagcgag ttggccgtgg cgatgaagaa gacgcgcgag aggtcgaacg gcaaatcgat
     2581 gtagtggtcg cggaacgtgt ggttctgctg cgggtcgaga atttccagaa gtgccgaggc
     2641 cgggtcgccg cgaaagtccg cgccgagctt gtcgatctcg tcgagcatca tcaaggggtt
     2701 attgaccgcg gcgcggcgca aagattggat gatgcggccg ggcatggcgc cgatgtaggt
     2761 gcgccggtgg ccgcgcagct ccgcctcgtc gcgcacgccg cccagagaga ggcgctcgaa
     2821 ctgccggccc aacgcgcggg cgatggacat ccccagagaa gtcttgccga cgccgggcgg
     2881 gccgacgaag cacaggatgg gactcttgga gtcgggccgg agcttgacga cggcgaggaa
     2941 ttcgaggatg cgctccttca cgtcttcgag accgtaatgg tcttcgtcga gaatgcggcg
     3001 cgcttcattg aggtcgagtt tatcttccga ggattttttc cacggcagct caaggatgta
     3061 ttcgaggtag gagcggatga cgtggtagtc gggcgcggcc tgtggcaggc gctccatgcg
     3121 gcgaagctcg cgctcggcct ccttacgcac gtcttcgggg aggtcggctt ggtcgagtcg
     3181 ctcgcgcatc atcgcggcct cggccgcttc gccgccctcg tcctcgccca gctccttctg
     3241 gatggctttc atctgctggc gcaggaagta gtcgcgctgc gccttctgca tctcgccctg
     3301 cgcctcgttg gagatgcgcg agcgcagctc cagaatctcg atctcgcgcg cgaggcggct
     3361 gtgggcgatc tggaggagtt cgtcggccgt gtcggcttcg agcatctgct gctcctgctc
     3421 gacgccgagg ttgaggagcg agccgaggaa gtaggcgatc tgcacggggt cgctcgtgtt
     3481 catcacggcg gcgcgcacct cgggcggaat ctcgggcagg agcgcgagcg cccgctggat
     3541 gagctgctgg acgttgcgga agagcgcctc gacgtgttcg tcgtcgcgct tggtcggcac
     3601 agggaggacg tggacgcgcg cgcgcaggtg cgggtcggtc tgcgaccact gaagcacctt
     3661 cacgcggtca acgccctgca cgatgatctg gacggcctcc tcggcgcgca tcatgcgttt
     3721 gatggtgacg agtgtgccca ccgtgtagag atcgtcgggc gggacggctt cggcttcgag
     3781 cgcgcggccg tcgcgcacgg tgacgcagac gacgagcttt tcctccgtgg cgagggccgc
     3841 ctcgacggcc gagaccgagc ggggccttcc ggcggccagc ggcacgatgg tgccggggaa
     3901 gagcgtcgtg ttctgcaagg ggagcaccgg aatctcgaag acgcccggct cgcctccgct
     3961 cgattgcccc ttcgcggggg cgctctgctc cggctccggc ttcggctccg cgtcggccga
     4021 cgggggcgcg ccccgacccc gcgcgacctt cggcgggggc ggcgtatcag cagacttgcc
     4081 gctcatctcc acggctcgct caacgtcgtc gaatgtgtct tcgggcatat caaaaattct
     4141 ctccccgtcc ggcgcgctac gggcctcact cttcgcagtg ttcggaactt ttaaggtaga
     4201 ggatgagcag gccgtcgcgg tagtcgcgct cgacggcggc gccctcgatg gggcagggga
     4261 agcggatcgt cttctcgaag cggctgtagg tgatttcgat ctgctggcag accatgccct
     4321 cggtgcagac cgtgtcgcgc cggcagccgc ggacgctgag ctgagcgccg ctgatttcca
     4381 cttccaactc gtcggcgcgg atgccggcca gctccagctt gacgatccac ccgtcgcgcg
     4441 tccggtacac gtcggccggc ggcgcccaca gccgccccgc cggcagcatc ccgtccgcct
     4501 tcggcagcat tcgcaggtag ccttcgattg aacgagccat ttgataagtg atgagtgata
     4561 agtgatgagt gatgagaaag agataagagg cggccgcctt ttcttatcac ttatcactca
     4621 tcactcatca ccagctctta gccgaaaacg cggttccaga acgtggagcc gctggagatt
     4681 tcttcgaggg cctcggcgag ttcgtggtcg tcctcggttt cgagggccac gtcggccatc
     4741 gagatgatgc cgcgcagcgt cccgtcgcgg tcgaggatgg ggatgcggcg cacctgcttg
     4801 tcgcccatct tgcgcacggc ctcgacgacg cggtcgttcg ggtgggccgt gtggatgtcg
     4861 gtcgtcatga tctcctcggc gcgcacggtg cgcgggtctt tgccctcggc gagcgcccgc
     4921 acgacgatgt cgcggtcggt gatgaggccg acgagcttgc cgctgctgcc cacgacgttc
     4981 gcctcgacgc ggttgatgtc gggccggtcg gtctcggcaa ggtcagtgag gttggccgcg
     5041 cggtcgatga cgggaatgac gcccgtgtcc tcgtcgcgca tcatgcgggc cacgccttcg
     5101 agcgtggtct cgcgggtggc caccgtcacg tcgcgcgtca tgatgtcgcg gcagcggacg
     5161 tgcgagcgcg aggggccgcc gggacggcgc gcgggacggc cgccgaaagt gcgtcggaaa
     5221 aagtcgcgtc cctcgtcgag gatggagcgg ccgccgccgc gctcaccacc gcgctcgtac
     5281 tcgcccgagt cgtcttcctc gtagcgccgg cgggcgatgg cgccgctgcc agcggtgcgg
     5341 cgcaccaccg tgcgctcttc atcgtagtcg ccgcgtccgt aacgcggttc atagtcggcg
     5401 cggccgtagt cggaaggcgg gcgctcgtag tagcgttcgc gctcgcgacc gccgccgccg
     5461 tagtcttccg agccgccgcc acgctcgtag ccgccgccgc gcgtgggcgg ggccgcctgc
     5521 cggtcgtcgt cgtaaaattc ctcgcgccgg cgctcactgc gcccgcgttc gctgttgtag
     5581 tagtcgtccc tgtcgtcgcg atatcttgac atctcgtcct cccatgaatg atttgccgca
     5641 gccgaggccc cgcggccgaa gacaagcagt agccgcggcc gacccgtccg gggcggcgcg
     5701 tcttcgagac acaccgaccc gtggccggga ttctctatcg tttcagcatc aaggggaact
     5761 ttagcgaggc gattatacca gagtgagcag cgagcagtaa aaagacagtg agcagtgagc
     5821 agtgagcagt aagcagtaga aaagaaacgc ggccgggttg actgaacacc ggccgcgttc
     5881 cgattgtttt tactgcttac tgctcactgc tcactgctca cttcttcacg gtcgcatttc
     5941 gacctccgcg acgagctgca cctgtgcgcg gatgtcgagc gagccgacct cgacgggcgt
     6001 gtcggccgac gcctgcatct cggccatgcg gccgaccgtg ccgcgtgcta aagggacggg
     6061 cgggcgatag agcggcccct cctgcacctc gacgatgcgg acgacgcgcc cgcccagcgc
     6121 ctcggctaaa gtctgcgcct tggcccgcgc gtcgcgcgtc gcctcggcga gcgcctgact
     6181 gcgcgcgggg cggtcgcggc gcagagtgaa gttaagataa tcgaccgtgt tggccccggc
     6241 gcgtgaggcg gcgtcgatga cggcgccgac cttcgtcaaa tccgacatcg tcacgatcac
     6301 gctgttacgc acctgatagc cgttgatctg cggcgactcg ttgggcttgt agctgtactg
     6361 cggctggagg ctgtagccgc ccgtcttgac ctccgcgccg gcgccggccg ccgccttgac
     6421 ggcgcgcacc gtcgcctccg tccggctcgc gttctccgct tgcgcctcgg acgcgctcgc
     6481 gttctgcgtg acgacggcga tggagacgat ggccgtgtcg ggctgcgcct gcacggtggc
     6541 ctcgcccgag acggtaatgc gcgtcagctc ggcgacggcg gcagcagcgc cgcccgtgag
     6601 cgcgccgttg ccggacgccg cggcggactc ttccgcgcgc ttgcccgtag gccgcagggt
     6661 gcgcgcctcg aacggcgtgc cctccatgta aatcgtgatg gcgtttttcg tctcgccgac
     6721 ggcttcgacc tccgcgtcct tgcgaaacca gctctcaccc ttgaagcggt cggcgttgat
     6781 gagcagatac ttctgctcgc ccgcgacgat cacccagccg cccgactcca cggtcgggcg
     6841 cagctggccg cgcaccgtaa tctctttcga cgcggccgcg gcctgcgccg caaacacgca
     6901 gcagatggcg agcgccgccg ccgcggtaaa aaatttgttc ttcaacattg ccgtaaaccc
     6961 ctttcttttt ttcgtcgtgg acgccccgaa ggggaaagag tatttgagac gtcggggagg
     7021 cgtcctcgct cgaatgaacg cgcggggcgc gcacgccttg cgccggcggc cgtcgcgtcg
     7081 ccctggtttg ccgggtcttc cgacagtttg ttacgatttc gcgagcgatg caaacactcg
     7141 gcccgaaatt tccaaactct cgacggagcg cgcgcaccgg cgaactcgtc acggtcggac
     7201 gcgtcgagga cgtgcccgag gggcgcggcg cgaccgtcga actcaagggc gggcgggagc
     7261 ttgcgctcta caacgtgtgt ggccgcttcc acgccgtcga gaacttctgc ccgcacaagg
     7321 gagcgccgct cgccgacggc gcgctgtgcg gcccgacagt cacctgcgac tggcacggct
     7381 ggcgcttcga cgtgactacg ggcgcgtgcc tcaaccgtcc gggcgagtcg attgaggctt
     7441 acgaagtcgt catcgaggac gggtggatta agatacgcgt ctgacgagga ggggtaagcg
     7501 aggagggtca ggggtaaagg ggaaggggta agggaaagac aacttcttct cccttacccc
     7561 ttccccttta ccctttaccc acaaagaccc tcagccgtcg ctgccgatgg cctcgacggg
     7621 acaggccaaa agcgcctctt cgcacagcgc gcgctcgtcc gcgctcgtgg gctgcttgta
     7681 gacgaaggaa tagccgttgc tgtcggcgcg ggtgaaattg tcgggcgccg tgtctcggca
     7741 cacgtcgcag tcgatgcact gcatatccac gtagaaagtg ccggaagcgt tctccggcac
     7801 gcggtcgttg gggtcagcca taagaaagtg ataagtgatg agtgataagt gatgagaaag
     7861 agataagaag cgactgtctt ttcttatcac tcatcactta tcacttatca ctttatttga
     7921 agcatgcggt cgagggcgac gcgcgcccaa gccgctgtct cctcgggcac ggtgatttgg
     7981 ttgacgacgt ggcccgcgag gagcgactcc agcgcccacg cgaggttctg tggcgcgatg
     8041 cgatacatcg tcgcgcacat gcagaggtcg ggcgcgagca gacggatttc cttgtcggga
     8101 aattgtttga cgagccggtt gacgaggtta acctcggtgc cgaccgccca gcgcgagccg
     8161 gccggggcct cgcgaatcgt cttgataatg aactcggtcg agccgctcag gtcgctcatc
     8221 tcgacgacct cgcgggtgca ttcggggtgg acgagcactt tgatgccggg cgtctcacgt
     8281 cgcacctgct cgacgtgcca cgggcggaag cggccgtgca ccgagcagtg gcccttccag
     8341 aggatgatgc gcgcgcggcg caactgctcc tcggtattgc cgcccaactc ctgatgcggg
     8401 tcccagagca ccatctcgtc ggcggggatt ccgaagcggt ggccggtgtt gcgcccgagg
     8461 tgctggtcgg ggaagaagaa gagcttgtcg gcctcctgca aatacttctc aaacagcggc
     8521 acggcgttcg acgaggtgca gaccacgccc tcgtgctcgc cgcagaacgc cttgatggcc
     8581 gccgacgagt tcatgtaggt gacgggcacg acgcgcgacc cctcgccgat gatccctaac
     8641 gcttcgagct gatcccacgc gtcctccact tgctcggccg tcgccatgtc ggccatcgaa
     8701 cagccggcgc cgaggtcggg cagaatcact ttctggtcgc ccggcttgag gatgtcggcc
     8761 gactcggcca tgaagtggac gccgcagaag acgatgaact ccgcgtcggg gcgcttcgac
     8821 gtgagctggg agagcttgaa ggagtcgccg gtgtagtcgg cgtggcggat cacgtcgtcg
     8881 cgctggtagt gatggccgag gatgacgagg cggcggccga gctgtcggcg gacggtctcg
     8941 atgcgctcgc gcagctccgc gtccgacagc ccgaagtagt cttcgagcgc gggggcctcg
     9001 ggcctggtct cggtgagaac tgtcatccgt ctgccctccc tgtttgccgt gccacgctca
     9061 gcacagacgg cgcaagcggc gcctgcgcga aacgcgaaat atagaacttt aagatacctc
     9121 gtgcaagcgc ccatccgaag gagcgccgcg cgagcaaaat ccgcgaacgc gtgcggcagc
     9181 ggccagtcat gcgcgttcat ggatttagat gccccggccg gtgctttaga tgtgtccggg
     9241 ccacaagcgc gtctcacttc agcgggtgat ggaaggacga cggggcggac ataaaaaaat
     9301 gagttctcct gagggagaag ttaaggtata aatgcgtagc caaagcaaaa tttgctgctg
     9361 ccggaacagt gtcgctcgcg aatgaaggct cgacctgtat gcgtcccaaa gaaattcatc
     9421 cccgcccccg cgtcaacaac ccatgacacg gaagctctct ggttagtaaa ccgataaccc
     9481 ggccagacaa tttctgccaa actcaacgtc aatgttcaga gcgccggccg ccggcgagat
     9541 gcccaacgag cctgaagggg gcttgtcagc agtgagtctc ttgcaccaat tccaccgaag
     9601 gagaagaaga atgaaaaaca atcagcgcgc gcgcttgtcc cgcgtctccc gcctcgcagt
     9661 gttcgccctg ctgggcgtca cgctcgtcgc cggcgccgca gtctggggcg tgcccggggc
     9721 ctcggccaac caccccgtct tagtcgaggg caactgcgac agccccgtac cgggcactaa
     9781 tctcgtcgaa aagggcacct gcggtgactt cgacggcgac ggacgcatcg gcacggccga
     9841 ggacaccgac ggcgccgacc gcatcttcgg caccatcaac gccgcgctcg gccccggcac
     9901 gggcgccgcc gccggcacgg gcgccaacca caacgggcgc atcaccatcg tcaagtcggg
     9961 tcgcttcgcc gagcagatct acatcgggca gaacctgcaa ggggcaggcg gccccggcgt
    10021 cgccatcccg ggcaacgtga cgattgaggc cgcgcccggc gtcgaggcca acatcgacgc
    10081 cattctgcaa ggcgaccccg ccggcgggaa cgttgcgcgc acggcgggca cgggcatcca
    10141 gatcgtctac acgaacgcca gtcgcgtagt cacactgcgc aacctcatca tccgcaactt
    10201 cgccaacggc atccagacct ttctcgactc gcgcgtccac atcgacaact gccggctgga
    10261 gaacaacctc aactacggca tccaggtgac caccaactcc cgcgtcgtca tcagcaactc
    10321 gcggattgac gggacgggct atcgcaccgg catcggttcc agcgtccctg ttcccggcac
    10381 cggcattctg gtcgagaaca acgcgaaggc attgattgaa aacacgacca tctcgcacag
    10441 cgccgggacg gggattgaca acaccaccgg gttcacagcc aacgtggtgc tcttcaaggt
    10501 cggctcctac ttcaacacct cgaacatcgg caacaacggc gcgttggtcg cgcccaaccc
    10561 cggcttcgcg acaatagact aagagcgagc ggagccgatg acctccaacg aagaagaagc
    10621 tccggggcgg gtatgttatc ccgccccgga gcttcttcta ctttaccgcg gcggccgctt
    10681 cggcggcggc aaggccgtcc ccggccccac agctaggggc gcgtcatctc ctccggcctg
    10741 acgtactcgt cgaattgctc gcccgtttcc ttcgttcgca taaaggagcg gttaagtcgc
    10801 aaatcgcgga ggcgctcgac gatctctatt ttttcttcaa atgaaagctt tgccagccgc
    10861 cgccggcgcg tctgcctttg ctcgaataac ccagccattt cgatagggtc tttcatacgt
    10921 cgaatctgtt cctgaattcg cgccatcttt cggccagccc gtgcctcgcc agaacacctt
    10981 cgagcgcgga caactcaacg gccccctctt cgaggaactg gatgacgcgc gccaaatctt
    11041 tcgcgcgccc cgtctccaac atcaccgcca ccagatgctc cgcacgcatg acgcgggtcg
    11101 gggttttctt aaacctgatc tcgaccgcct gcgtcagggc ctccgcgagc agcggggtga
    11161 acaccggaag aaactgcacc ggccagcctt ctatttcgac cgcctcgccg gcaggttcgt
    11221 acccgagggc ggcgaggtag tcgtagagtg gcgtgagcga gagcagtgcg tcggacgccc
    11281 cctcggcggc gaagaagaca tccacgtcga acgtggagac aggttcgacg taaaacgtgg
    11341 ccgcaatcgc gccgccgata gcgtagcggc cgacgacgcc ctcggcctcc atccggttaa
    11401 gcacctgaag cgttttctcc acgctgtttc cgccagcaga ccgtgcgccc ctttggcctt
    11461 cacgggtgcg tcatctcctc gggcttaacg tactcgtcga actgctcgcc cgtcagaagc
    11521 cccagctcga cggccgactc gcgcagggag atgccgcgcc ggtgggcgtt cttggcgagc
    11581 ttcgcggcgt tgtcgtagcc gatgtggggg ttgagcgcgg tgacgagcat gagtgagttg
    11641 cgcaggttct cctcgatgcg ctcgcggttg agcgtgatgc cttcgacgca gtggtggacg
    11701 aagctgcggc aggcgtcgcg caggaggcgg atggagtgga gcaggttgtg aatcatcacg
    11761 gggttgaaga cgttcagctc gaagttgccc tgcgagccgg cgaacccgat ggcggcgtcg
    11821 ttgcccaaca cctgcacggc gaccatcgtc aacgcctccg actgcgtcgg gttgaccttg
    11881 cccggcatga tcgacgagcc gggttc
//
LOCUS       DASVES010000422         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1624285, whole genome shotgun sequence.
ACCESSION   DASVES010000422 DASVES010000000
VERSION     DASVES010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624285"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..1585)
                     /locus_tag="VF240_17335"
     CDS             complement(422..1585)
                     /locus_tag="VF240_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177025.1"
                     /translation="MRAKHKIATGRVRRTGKAATGGAIVCVFYLVAVFADFLAPNDFR
                     EQSRREPSSPPTVLHFRDERGDFHARPFIHPRRLTDPLHRLYSEDTSRRYALTLFARG
                     YRYKLFGLVETDRHLIGLRPEGDAVSASAGDAGAASTDNIKSAAAVASSSDAAPRLYL
                     LGTDGLGRDRFARLLRASRFTLLVGPLGTLLASLLGVALGCAAGYAGRAVDSVLMRVA
                     DTMMALPTLVIIIAARAAFPLELPPTRAAVLLVGLFVLTGWAEMARLARGLILALRRR
                     EFVLAAESLGLGRGRVLFRHILPNAAGPLLVQATLMLPAFLLAETALSYLGVGVQEPE
                     PSWGNMLTAASDISLLGESPFVLLSPALCIFLFVLGVRLLGDGLRVGQEKTSA"
     gene            complement(1582..2550)
                     /locus_tag="VF240_17340"
     CDS             complement(1582..2550)
                     /locus_tag="VF240_17340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177026.1"
                     /translation="MRSGVLKLIVSRAAQGLVVLVVVSALTFALLAAAGGDALDAMYN
                     DPHVSLEVIESLRASYGLDRPLPVRYARWLTGALRGDLGFSFYYRAPVGSLLLPRFLH
                     TLVLGALALSIAWGLALALGALSARAPRGWADRSAGALILLASSTPRIVLALLALVVA
                     ARWALFSTGDGGGGGGKPMRALLAALVLSAPLVALFLAQTREGLREALGEEFVRVARA
                     KGLSERRVVFAHAMRAALNPLVTVFGYSLGGVVSGSVIVETILGWPGLGQLSVTAVRS
                     RDVPLLMAVVVVTSTAVLVGNLIADILLRLNDPRIGWDESGKAAGA"
     gene            2716..>4344
                     /locus_tag="VF240_17345"
     CDS             2716..>4344
                     /locus_tag="VF240_17345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7177027.1"
                     /translation="MTTRFRRPRRNAFILTLLLLLPMGACQQATAPPANTTNTATAGE
                     RTAGVRGGSLTYRLTEPPTGFNPVMASREADFVVSFFLAAGRLIEFDHDKRSYAPSLA
                     ESWRLLEDGRTVELTLRDGLKFSDGQPLSTADVEFTMRAIYSEPSSIIRQSLLFSERP
                     AETRVLDARRFQLVFPEQVAAPETYLVNIPVLPRHRLEEELRAGNFGKAYGVTSDPQQ
                     IVTSGAFAFESVAPGERVRLKRNPHYWKKDSAGTQLPYLDNIAIEIVGDQNAALTRLR
                     EGGLDLVDRIRPSDYASLQQQTGGVRAVDLGPGLSTDHIFFNLNEGARPPRAVADKQA
                     WFKDARFRRAVSHAIDRDSVASGTLQGLATPLYNFVSPANREWVAADVPRTEFSVERS
                     RALLAEAGFSVRGTAEAPELFDAGGRRVEFTLIVPQESGPRVSTAAAVQEDLAKVGIL
                     VNVAPIETGELRRRTLQSYDYEAALFGLGITDFDPSSASGLLASGSPDHQWHPAQTKP
                     TTDWETRLDALVAQLARERDTERRRAVFRDIQLLM"
BASE COUNT          790 a         1566 c         1402 g          586 t
ORIGIN      
        1 caagtcggcc ggcccgcgcc ttcccccaca accaaaaacc cttgaagaaa aatttctatt
       61 ctgaatccga gcctgcgaac tttgcgtgcg actccgggga gcgactcttc tctgccaaag
      121 gggccgctcc ccggagccgc gaccacccga cgaattgcca ccccgacatg gctcctcgcc
      181 ggtggaatca aaagcccttg ccgacggccg tcggcttccg ggcctggcaa gcacgagaaa
      241 gattcccctc tcgcctgtcg gaagcagcga cccccatcac cgtccgacgt gcacaagtta
      301 cccaacctca cttgagaaaa acttgagtca agctggaaaa aatcaccccc cgtccccgcg
      361 acagaagaca atccgtttag caacggcatt tagcgaagtt gatgaggtcg ccgaaaaaat
      421 tttaggccga agttttttcc tgtcccaccc gcagcccatc gccgaggagg cgcacgccga
      481 ggacgaaaag gaagatgcag agggcgggcg aaagcaggac gaacggcgac tcgccgagca
      541 gcgaaatgtc cgatgccgcc gtgagcatgt tgccccagct cggctccggc tcctgcacgc
      601 cgacgccgag gtaagacagc gccgtctcgg ccagcaggaa ggcgggcagc atcagcgtgg
      661 cctgcacgag cagcggcccg gcggcgttgg gcaggatgtg ccggaagagc acgcgccctc
      721 gcccgaggcc cagactctcg gcggcgagca cgaactcccg gcggcgcaac gcgagaatta
      781 accctcgcgc gagccgcgcc atctcggccc agcccgtcag gacgaacaga ccgacgagca
      841 gcacggccgc acgcgtcggc ggcagctcca gcggaaaggc ggcgcgcgcc gcgatgatga
      901 tgacgagtgt gggcagcgcc atcatcgtgt cggccacacg catcaggacg gaatcgaccg
      961 cgcgccccgc gtacccggcg gcgcacccga gcgcgacgcc gagcaggctc gcgagcagcg
     1021 tccccagtgg gccgaccaga agcgtgaagc gcgacgcgcg cagcagccgc gcgaagcggt
     1081 cgcgcccgag gccgtcagtg ccgagcaggt agagacgtgg ggcggcgtcg gaggaagacg
     1141 cgacggcggc agcagacttg atgttgtccg tggacgccgc gcccgcgtcg ccggcagacg
     1201 ccgacacagc gtcgccttca ggacgcaggc cgatgaggtg cctgtccgtc tcgacgaggc
     1261 cgaagagttt gtacctgtag ccgcgtgcga agagcgtgag tgcgtagcgc cgcgaggtgt
     1321 cttcggagta gagcctgtgg agcgggtcgg tcagacgtcg cgggtggatg aaggggcggg
     1381 cgtgaaaatc cccacgctcg tcgcggaagt ggaggacggt cggcggcgac gaaggctcgc
     1441 ggcgcgactg ctcgcggaag tcgttcggcg cgaggaagtc cgcgaagacg gcgacgaggt
     1501 agaagacaca gacaatcgcg ccgcccgtcg cggccttccc cgtgcgacgc acgcggcccg
     1561 tcgcgatttt gtgtttcgcc ctcacgcgcc cgccgccttg cccgactcat cccagccgat
     1621 gcgcgggtcg ttgagtcgca ggaggatgtc cgcgatgaga ttcccgacaa gcacggccgt
     1681 cgaggtcacg acgacgacgg ccatcagcaa cggcacgtcg cgactgcgga cagccgtgac
     1741 gctcaactgc cccagccccg gccagcccag gatggtctcg acgatcacgg agccgctcac
     1801 cacaccgccc agcgagtagc cgaagacggt gacgagcggg ttcagcgccg cgcgcatcgc
     1861 atgagcgaag accacacgcc tctcgctcaa gcccttcgcg cgcgccaccc tcacgaactc
     1921 ctcgcccaaa gcctcgcgca gcccctcgcg cgtctgcgcc agaaagagcg cgacgagcgg
     1981 cgccgagagc acgagcgccg ccagcagcgc ccgcatcggt tttccaccgc cgccgccgcc
     2041 gtcgcctgtc gagaaaagcg cccagcgcgc ggcgacgacg agggcgagga gcgcgagcac
     2101 gatgcgcggc gtcgaggaag cgagcaggat gagcgcgccc gccgagcggt cggcccaccc
     2161 gcgcggcgcg cgtgccgaga gcgcccccag cgcgagcgcg agtccccaag cgattgacag
     2221 ggcgagcgcg ccgaggacga gcgtgtgcag gaagcgcggc aggaggagcg agccgacggg
     2281 cgcgcggtag tagaaggaga agccgaggtc gccgcggagc gcgcccgtga gccagcgcgc
     2341 gtaacgcacg gggagcggcc ggtcgaggcc gtagctggcg cgcaggctct cgatcacctc
     2401 aagggagacg tgcgggtcgt tgtacatcgc gtcgagcgca tcgccgccgg ccgccgccag
     2461 cagcgcaaac gtcaacgccg agacgacgac gaggacgacg aggccctgcg ccgcccgcga
     2521 cacgatcaac ttgagcacgc cgcttcgcat gagtcgctgc tgaaaagttc tcccgcgcgc
     2581 ccggcagtat atatcacgcg cagtttttag ttctggacac gcggcgaaca ttcgtgctcc
     2641 aatccgcccg aacctcaccg gcatcaatcg ggcgcgagac gctattcgac tcctgcccta
     2701 agccccgagg gaagcatgac gacaaggttc cgccgcccgc gacgcaacgc gttcattctc
     2761 actctcctgc tcctgctgcc catgggcgcg tgccagcagg caaccgctcc tccggccaac
     2821 acgacgaaca cggccacggc gggcgagcgc acggccggtg tgcgcggcgg ctcgctcacc
     2881 taccgcctga ccgagccgcc caccggcttc aaccccgtca tggcgtcgcg cgaggccgac
     2941 ttcgtcgtct ccttcttcct cgcggccggg cgcctcatcg agttcgatca cgacaaacgt
     3001 tcctacgcgc cgtcgctcgc cgagagctgg cgtcttctgg aagacggccg cacggtcgaa
     3061 ctcacattgc gcgacggtct caagttctcc gacggccaac cgctttcgac cgccgatgtc
     3121 gagttcacga tgcgcgccat ctactccgag ccgtcgtcga tcatccgcca atcgctgctc
     3181 ttcagcgaac gtccggccga gacgcgcgtg ctcgacgcgc ggcgcttcca gctcgtgttc
     3241 cccgaacagg tcgccgcgcc cgaaacctac ctcgtcaaca ttcccgtgct gccgcgccac
     3301 cgactcgaag aggaactgcg tgcgggcaac ttcggcaagg cttacggcgt cacctccgac
     3361 ccgcagcaga ttgtcacctc cggggccttc gccttcgagt cggtcgcgcc cggcgagcgc
     3421 gtccgtctca aacgcaaccc gcactactgg aaaaaggatt cggcggggac gcagcttccc
     3481 tacctcgaca acatcgccat cgaaatcgtc ggcgaccaga acgccgcgct cacgcgtctg
     3541 cgcgaaggcg gtctcgatct ggtagaccgc atccgcccgt cagactatgc ctcgctccag
     3601 cagcagacgg gcggcgtgcg cgccgtcgat ctcgggccgg ggctttcgac cgaccacatc
     3661 tttttcaatc tcaacgaggg cgctcgcccg ccgcgcgccg tcgccgacaa gcaggcgtgg
     3721 ttcaaggacg cgcgcttccg ccgcgccgtc tcacacgcca tcgaccgcga ctccgtcgcc
     3781 tcgggcacgc ttcagggcct cgccacgccg ctctacaact tcgtctcccc ggcgaaccgc
     3841 gaatgggtgg ccgccgacgt gccccgcacc gagttcagcg tcgagcgctc ccgcgcgctc
     3901 ctcgccgagg ccggcttctc ggtgcgcggg acggcggaag cgccggagct tttcgatgcc
     3961 gggggccgcc gcgtcgagtt cacgctcatc gtgccgcagg agagcggccc gcgcgtgagc
     4021 acggcggcgg ccgttcagga agacctcgcg aaggtcggca tactcgttaa cgtcgcgccc
     4081 atcgaaacgg gcgaactgcg gcggcgcacg cttcagagct acgactacga ggccgcgctc
     4141 ttcggtctcg ggatcaccga cttcgacccc tcctcggcct cgggattgct cgcctcgggc
     4201 agccccgatc accagtggca cccggcgcag acaaagccca cgaccgactg ggagacccgg
     4261 ctcgacgcgc tcgtcgccca actcgcgcgc gagcgcgaca ccgagcgccg tcgcgccgtc
     4321 ttccgcgaca tccaattgct gatg
//
LOCUS       DASVES010000423         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_663320, whole genome shotgun sequence.
ACCESSION   DASVES010000423 DASVES010000000
VERSION     DASVES010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663320"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2950
                     /locus_tag="VF240_17350"
     CDS             <1..>2950
                     /locus_tag="VF240_17350"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7177028.1"
                     /translation="IDRKVALIDDQLTVISDEFQDRRGRITVKNYQIESADAGDKAEM
                     RQELQQMKAQKARFEMPSESGRNEQKEFDYAQLEGLYNDLKREKAALLTERGEKLKPV
                     SDLRRQRDEYLKNNVAGVTEDQVKKLLAKNENFEVKMRQINIQGDQVVDRCETCHLGT
                     REPLEIDAGDMMSAGRRNRPDQMAKAFVSHPSKELLQIHDPEKFGCASCHGGNGRATT
                     SIEKGHGRHRFWLHPMYERDNMEAGCQQCHMQDRVLVGSPILNKGKDLFTERGCVGCH
                     RYEGFDRETDSLAVARQEIKQLEEQVAANRREALSAQAKRDESGISDEENQRLLAQIE
                     SLTVTSSQIESRVDQLNIQSRYLMQDQKKVGPNLKDVRIKLVKEWIPEWLKDPQSFRP
                     GTKMPTFWYLSGKEGISVPPARQEEERKAISAYLWQSAFDGRLPQQPTGNAATGKQLF
                     ETRGCLACHSIGDGESVIGAEFAANLQKVGQKANYEYLVRWVHNPRERTSPYCPKERK
                     DLTPADYAAKNLPYVFDANHSKCPNDGAELQIQNMTVMPNFRLSDQDSRDIATYLKSL
                     SPPATYPDASYMDDPALKAKGGELIKTYGCAGCHEIRGFEEEQRIGKELTTEGATPLE
                     RLDFALMTHEAEDGHDPLNLYPEKKNERWYNHKGFFERKIEDPSIYDRGKEKEPQDHL
                     RMPEPFMKDDWRAALTTFLLGSKGDEGSNVPKSFYYTPNSLQKDIQDGWWVVKKYNCM
                     GCHQIQVGQERPALTRLPWYEEAGELGKRQWPPGLLTEGARVDPNWLLRFLRDPSLSG
                     LSEEELTAHSAGAGATRAGGRASAGGASGATTARPAGAAATASTAQLQPAAPASGQAS
                     QPAAGGSALGLPPQPGENINGLRRNLAPRMPTFNFSPNELRILVRFFMAYASQQEPYI
                     KERIEPLTPEETSVARAFFSSGTQPCLSCHLTGDPTHDQTAYAPNFLDAAQRLKPAWT
                     LRWILDPPKIW"
BASE COUNT          631 a          977 c          919 g          423 t
ORIGIN      
        1 catcgaccgc aaggttgcgc tcatcgacga ccaactgacg gtcatctccg acgagtttca
       61 ggacaggcgc gggcgcatca ccgtcaagaa ctatcaaatc gagtcggccg acgcgggcga
      121 caaagcggag atgcgtcagg aacttcaaca gatgaaggcg cagaaggcgc gcttcgagat
      181 gccttcggag tcgggccgca atgagcagaa ggagttcgac tacgcgcagc tcgaaggtct
      241 ctacaacgac ctcaagcggg agaaggcggc gctcctgacc gagcgcggtg agaagttgaa
      301 gccggtctcg gatttgcgcc gtcagcgcga cgagtatttg aagaacaacg tcgcgggcgt
      361 caccgaggat caggtcaaaa agctgctggc caagaacgag aacttcgagg tcaagatgcg
      421 gcagatcaac attcagggcg atcaggttgt tgaccgctgc gagacctgtc acctcggcac
      481 gcgcgagccg cttgagatcg acgccggcga catgatgtcc gccgggcggc gcaaccggcc
      541 cgaccagatg gcgaaagcgt tcgtcagcca cccgagcaag gaactgctcc agattcacga
      601 ccccgagaag ttcggctgcg cgagctgcca cggcggcaac ggccgcgcca ccacgagcat
      661 cgagaagggc cacggccgcc accgcttctg gctccacccg atgtacgagc gcgacaacat
      721 ggaggccggc tgccagcagt gccacatgca ggatcgcgtc cttgtcggct cgcccattct
      781 caacaagggc aaagacctct tcaccgagcg cggctgcgtc ggctgccacc gctacgaagg
      841 cttcgaccgc gagactgatt cgctcgccgt cgcccggcag gagatcaagc agctcgaaga
      901 acaggtcgcc gccaaccggc gcgaagctct ctccgcgcag gccaaacgcg acgagtcagg
      961 catcagcgac gaggagaacc agcggctgct cgcgcagatc gagtcgctga cggtgacgag
     1021 cagccagatc gagtcgcgcg tcgatcagtt gaacatccag tcgcgctact tgatgcagga
     1081 tcagaaaaag gtcgggccga acctgaagga cgttcgcatc aaactggtca aggaatggat
     1141 acccgagtgg cttaaagacc cgcagtcgtt ccggcccggc acgaagatgc cgaccttctg
     1201 gtatctgtcg ggcaaggagg gcatcagcgt gcccccggcg cggcaggagg aggagcgcaa
     1261 ggccatctcg gcctacctct ggcagtcggc cttcgacggg cgtctgccgc agcagccgac
     1321 gggcaacgcc gccaccggca aacaactctt cgagacgcgc ggctgtctcg cctgccactc
     1381 catcggcgat ggcgagtcgg tcatcggcgc ggagttcgcc gccaatctcc agaaggtcgg
     1441 acagaaggcc aactacgaat atctcgtccg ctgggtacac aacccgcgag agcgcacctc
     1501 gccctactgc ccgaaggagc gcaaggattt gacgccggcc gactacgcgg cgaagaacct
     1561 gccctacgtc ttcgacgcca accactcgaa gtgtccgaac gacggcgccg aactccaaat
     1621 ccagaacatg acggtgatgc ccaacttccg tctctcggat caggactcgc gcgacatcgc
     1681 cacatatctc aagtcactct ctccgccggc cacctacccg gacgcgtcct acatggacga
     1741 ccccgccctg aaggcgaagg gtggcgagtt gattaagact tacggctgcg ccggctgcca
     1801 cgagatacgc ggcttcgagg aggagcagag aatcggcaag gagttgacga ccgagggcgc
     1861 gaccccgctc gaacgcctcg acttcgcgct gatgacgcat gaggccgaag acggtcacga
     1921 cccgctcaac ctctaccccg aaaagaagaa tgagcggtgg tacaaccaca agggattttt
     1981 cgagcgcaag atcgaagacc cgtccatcta cgaccgtggc aaggagaagg agccgcaaga
     2041 tcacttgcgg atgcccgagc ctttcatgaa ggacgactgg cgcgccgcgt tgacgacgtt
     2101 cctgctcggc agcaagggcg acgagggcag caatgtgccg aagtcgttct attacacccc
     2161 gaacagtctt cagaaagata ttcaggacgg gtggtgggtc gtcaagaagt acaactgtat
     2221 gggctgccac cagattcagg tcgggcagga gaggcccgct ctcacgcgtc tgccgtggta
     2281 cgaggaggcc ggcgagctgg gcaagcgcca gtggccgccc ggactactca cggagggcgc
     2341 gcgcgtcgat ccgaactggc tcctgcggtt cctgcgcgac ccctcgctca gcgggctttc
     2401 ggaagaagaa ctgaccgccc acagcgcggg cgcgggcgcc acgcgagccg gcgggcgcgc
     2461 gagcgcgggc ggcgcaagcg gagcgacgac cgcacgaccc gccggcgccg ctgcgaccgc
     2521 ctccaccgca caactccaac ccgccgcgcc ggccagcggt caggcgtcac aaccggccgc
     2581 gggaggctcc gcgctgggct tgccgccaca gccgggcgag aacatcaacg gtctgcgccg
     2641 caacctcgcg ccccgcatgc ccaccttcaa cttctcgccc aacgagctac gcatcctcgt
     2701 gcgcttcttc atggcctacg cctctcaaca ggagccgtac atcaaggagc gcatcgagcc
     2761 gctgacgcct gaagagacga gcgtcgcgcg ggccttcttc tcgtcgggta cgcagccgtg
     2821 tctgtcgtgt cacctgacgg gcgaccccac gcacgaccag acggcctacg cgccgaactt
     2881 cctcgacgcc gcgcagcgac tcaaaccggc ctggacgctt cgctggattc tcgacccgcc
     2941 gaaaatctgg
//
LOCUS       DASVES010000424         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_904301, whole genome shotgun sequence.
ACCESSION   DASVES010000424 DASVES010000000
VERSION     DASVES010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904301"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VF240_17355"
     CDS             complement(<1..740)
                     /locus_tag="VF240_17355"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7177029.1"
                     /translation="MLSVTGATSAESASVVAYATSTNNPYPRSPSKDALKWADAELKA
                     MTLDEKIGQLISVGINARFLNQESDEFRALLRQIEQNHVGGIILFRGPVYESVHLVNR
                     MQARARRPLLISADLEAGAGMRFEDTINFPWNMAVGATGDPTFARRQGEVTAREARAL
                     GVQQVFAPVADVNNNAANPVINVRSYGEDPAEVSRYVAAFVEGLQNGGVIATAKHFPG
                     HGDTSVDSHRGLPEISVDRARLDAVELVP"
     gene            complement(1072..>2202)
                     /locus_tag="VF240_17360"
     CDS             complement(1072..>2202)
                     /locus_tag="VF240_17360"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177030.1"
                     /translation="YFGGMEAVIWVEVVQLGIYLTGALAAALVLASAIGGGFGGAWAL
                     GEAAGKFDVIDMGLRQTNYSFGLPLTGRSLSFSLPFDLTKTYTLWAGLIGGCFLTMST
                     HGTDQYLVQRYLCTDRPRRAAAALLSSGAVVFAQFIGFLIIGVLLYAFYQPYNDPLYT
                     NYAESCARLLPDAAAKCLADPAFAQSATASFPFTGGDRVFPDFITRHLPSGLSGLVVA
                     AIFAAALSSSLNAIAATAVNDLYRPFRPRRDDRHYLRVSHWLTLLWGVAQILIALLFM
                     QTTRSALDQALSVASLFNGPVLGVFLVGAFLRRVGEPPALVGMITSMTVMLYIRFWTP
                     IAWTWYVLIGSAITFFVAWAASYAFAPAPLDKQDELAPDAAD"
BASE COUNT          425 a          658 c          760 g          359 t
ORIGIN      
        1 gggaccagct cgacggcgtc gagacgcgcg cggtcaacgc ttatctccgg cagcccgcgg
       61 tgcgaatcaa cactcgtgtc gccgtggccg gggaaatgtt tggcggtggc gatgactccg
      121 ccgttttgca gcccctcgac aaaggcggcg acgtagcgcg acacctccgc cgggtcttcg
      181 ccgtaggagc gcacgttgat gacggggttg gcggcgttgt tgttcacgtc ggccacgggc
      241 gcgaagactt gctgcacgcc gagggcgcgg gcctcgcgcg ccgtcacctc gccctgccgg
      301 cgcgcgaaag tggggtcgcc cgtcgcgccg acggccatgt tccaggggaa gttgatggtg
      361 tcttcaaaac gcatcccggc gccggcttcg aggtcggccg agatgagcag ggggcggcgg
      421 gcgcgcgcct gcatccggtt gacgaggtgg acggactcgt agacggggcc gcgaaagagg
      481 atgatgccgc cgacgtggtt ctgctcgatt tgccggagca gtgcgcggaa ctcgtcgctc
      541 tcttgattga ggaagcgggc gttgatgccg acggagataa gttgcccgat tttctcgtcg
      601 agcgtcatcg ccttcagctc cgcgtcggcc cacttgagcg cgtccttcga cgggctgcgc
      661 gggtagggat tgttggtaga tgttgcgtat gcgactacgg atgctgactc agcactcgtc
      721 gcccctgtca cgctcagcat gcctgaaggc gtgagcagag aagccgccag taagagcggc
      781 gtcagagttt tccgccgtag gtttccagta ctgatatgcc tgatcttcat gttagaaagc
      841 tcgaatcatc tgaccttgtg tctgtagggg cagggacaag tccactgccc gtgctgcgaa
      901 gcaattaaca tggatgggca ggatagttag gatgagaaga tttgaaatct gaaatctcaa
      961 atctgaaatc tcaaatcagc gctttcttca tcctacccat cctgtccatc catgttaatt
     1021 attcttcccc aaagacgggc aggaacaagc cctgccccta caaatgcaaa tttagtcggc
     1081 cgcatccggg gcaagctcgt cctgcttgtc cagcggggcg ggcgcgaagg cgtagctcgc
     1141 cgcccacgcc acgaagaagg tgatggcgct gccgatgagc acgtaccacg tccacgcaat
     1201 cggcgtccag aagcgtatgt agagcatcac ggtcatgctc gtaatcatcc cgacgagcgc
     1261 gggcggctcg cccacgcgcc gcaggaaggc gccgacgagg aagacgccga gcacggggcc
     1321 gttgaagagc gaggcgaccg agagcgcctg atcgagcgcc gagcgcgtgg tctgcatgaa
     1381 gaggagcgcg atgaggattt gcgcgacgcc ccacagcagc gtcagccagt gcgagacacg
     1441 caggtaatgc cggtcgtcgc ggcgcgggcg gaacgggcgg tagaggtcgt tgacggccgt
     1501 cgcggcgatg gcgttcagag acgacgagag cgccgccgcg aagatggccg cgacgacgag
     1561 acccgaaagg cccgacggca aatgtcgcgt gatgaagtcg gggaagacgc ggtcgccccc
     1621 cgtgaacggg aacgacgcgg tcgcggactg cgcgaaggcc gggtcggcga gacacttcgc
     1681 ggccgcgtcg ggcagcaggc gcgcgcaaga ctcggcgtag ttcgtgtaga gcgggtcgtt
     1741 gtagggctgg tagaaggcgt agagcaacac gccgatgatg aggaagccga tgaactgcgc
     1801 gaagacgacc gcgccgctcg acagcaacgc ggcggccgcg cggcggggcc ggtcggtaca
     1861 caggtaacgc tgcacgagat actggtcggt gccgtgcgtg ctcatcgtca ggaagcagcc
     1921 gccgatgagt ccggcccaga gcgtgtaggt cttcgtcaaa tcgaacggca agctgaagct
     1981 caggctccgc cccgtgagcg gcaggccgaa cgagtagttc gtctggcgca atcccatgtc
     2041 gataacgtcg aacttgccgg cggcctcgcc gagcgcccac gccccgccaa agccgccgcc
     2101 gatggccgag gcgaggacga gcgcggcggc gagcgcgccc gtgagataaa ttccgagctg
     2161 cacgacctcg acccagatga ccgcctccat cccgccgaag ta
//
LOCUS       DASVES010000425         7517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1534983, whole genome shotgun sequence.
ACCESSION   DASVES010000425 DASVES010000000
VERSION     DASVES010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534983"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..2974)
                     /locus_tag="VF240_17365"
     CDS             complement(116..2974)
                     /locus_tag="VF240_17365"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEX7177031.1"
                     /translation="MAFAVLVGLLALALYPHQGSATFRRAASTAAEAGSDSTSSPASV
                     STSAPGAGLNGAAGHSSDSPFSTAAAAPPAALFMQAGPEDVESFAADCVTPKTSFAAG
                     ETACAKATNLSGVLGFERVVYWISPEGTILQTDSVSTASPTATRVVGARGGWKLYLST
                     ASDGSLRKASFFSVSDAANKAVDLSVYDAIDRRISSYAAGHSITYLVVVTNTGPDAAT
                     DVVLTETPANNATFDTVPPVQQSGPAFTCTRPSGSTCTLASMAAGETAEFAFVYTVDT
                     GTAAGTEVNHSVTVTNDLEEEEELHEGDNESTATTNITGEVTAATCALNCPDDITVVA
                     NATQGGNPGAFVTFSSAEGLGDCGEISSNTPSGSFFPLGTTNVSVSSTEGAGACTFDV
                     TVVTAGAPTVTCPGDVVVAAPAGESEATVNPGTPSTSPSSGVSVSGQRSDNELLNAPY
                     PIGTTHITWTATDTSNGLSSTCTQKIIVTSDTCADDETDPAINAPPDVLLDTQAEQTG
                     VCGLIVPESQLGTPDVSDNCVVNVARTGVPAGNFFPIGATTVTYTATDAAGNTATDTQ
                     IITIRDKTPPVIYAPANATYVCPSEVPAASASQAGGPLLDNTGQLVRDASGDLVPGGQ
                     PYDSCGAPTVGVTQTDNGGAGSVANPLVITRTYTATDSHGNTASASQTITVADGAAPT
                     IALDGANPQVVECHTSYTELGATASDNCSANFAATPSGTVNVNVPGTYTITYNATDAA
                     GNAATPVTRTVNVVDTTAPVMSCPADIVVYLPLNSTAVSMPVSFAPTATDSCDASVPV
                     TTTIASGSIFNVGTTNVTASATDDAGNTSSCSFNVTVLYNFTGFFSPVDTVPTLNVVK
                     AGSAIPVKFSLSGNKGLGIFAVGSPASGTIPCDAQAPLDPVEETVTAGGSSLNYDAAS
                     DRYSYIWKSEKEWANTCRQLVITLNDGSVHIANFKFTK"
     gene            3613..4629
                     /gene="rfbB"
                     /locus_tag="VF240_17370"
     CDS             3613..4629
                     /gene="rfbB"
                     /locus_tag="VF240_17370"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7177032.1"
                     /translation="MRKLFVTGGAGFIGSAFIRLLLDESPDCLVTNFDALTYAGNLDN
                     LSGLDGDSRHRFVRGDIGDRAGVLSALEEGTEAIVNFAAESHVDRSIASADEFIRTNV
                     LGTQVLLDAARERGVKKFVQVSTDEVMGSLPDDDTAFFTEASPFEPNSPYSASKAAAE
                     HFVRAAQHTHGLDTVVTRCGNNYGPRQFPEKFLPLIISNAMRDEPLPLYGDGRYTRDW
                     IYVEDHCRAILMVMQKGRSGAAYNIGARNERRNLEVAESVLRKLGKPLSLIRHVKDRP
                     GHDRRYAIDPSRVETELGWRPRETWETGLEKTVRWYAENGEWVERARSGAYREYYEQQ
                     YGKI"
     gene            4722..5576
                     /gene="rfbD"
                     /locus_tag="VF240_17375"
     CDS             4722..5576
                     /gene="rfbD"
                     /locus_tag="VF240_17375"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX7177033.1"
                     /translation="MKILVTGAGGMVGRALVKHCEGRGDEVRPFDRAALDIADERAVS
                     ETIGRERPEAVVNCAAWTDVDGCEDDPARAHAANALGPEHLAAACRRAGAVLVTISTD
                     YVFDGRKSGFYTQRDEPNPQSVYGRAKLEGERRAQSACARSIVVRTGWIFGEGGKSFL
                     SRVVETGRRGERLKAIQDAWGTPTYADDLAARLRRLAELDLPGVYHVANAGDGTSYYE
                     FTRAALGAAGGDVSLIEGVSVDSLSRPAPRPRNSRLRCLLSEAVGLPPLPDWREALAR
                     FVAVGARE"
     gene            5776..>7517
                     /locus_tag="VF240_17380"
     CDS             5776..>7517
                     /locus_tag="VF240_17380"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7177034.1"
                     /translation="MMSEDNRLLPVTTIDRALDRPLSDLAASAKGGYGASSNDPTHLR
                     DYLNVVLKRKWLILSLVLVVTTLVTIQMYRMPTVFEAETTLQIDPKRESILARKDQIV
                     INTGRGGGDAVYAQTQLRLLENRALARQVIRTLDLQNNPAFLGDQAQASFVSSLRRIF
                     LREKKGTRSTAEDQSMGTIPEVTWENSRDEKLTAEELRRFEPYEDTLAANLKVEPETG
                     TNLVKLRYRHTDPEMAQKIVNTLANIFAYNNFDRETSGSDRNTRVLAEKIAELQLKIT
                     QLEERKYNYAKANNLPFDTKQGSDLEAERLAILSRQVLEAENLLKEAETQWNEAKKGD
                     PQTIPQVQDNQRVQKLRNQLDELEQQKAELMEVYTEEHPQVKKLDKKITALSASMKKA
                     PTDIVSSLEKRYRAAQEKVSKLKTDYFRQKGTTTTQSLAMIQYGTILQDLETNKQDLN
                     VLRQRLREIENVNVDRINNVSVTTYSRRGNPVGPPRVRNIMLALLLSLGAGVGLAFLL
                     DYLDDTLKSVDDVDRYLHLPSLALIPATRGESRSRLKSGGPAPAEGEVTALALTTDVS
                     SPVAEAYRHLRTSLL"
BASE COUNT         1298 a         2440 c         2500 g         1279 t
ORIGIN      
        1 gcccgggctg gtttcgcgtg aactgaacac cttatctgtc ggatcatttc ctcaaggtag
       61 aaggcttggg ccgtcgcggg cggggccgcg gccccgcctg cgacggcctt ccgcctcact
      121 tggtgaactt gaagttggcg atgtggacgc tgccgtcgtt cagcgtgatg acgagctgac
      181 gacaggtgtt cgcccattcc ttctccgact tccagatata gctgtagcgg tcgctggcgg
      241 cgtcgtagtt caggctgctc ccgcccgccg tcaccgtctc ctcgacggga tccagcggcg
      301 cctgggcgtc gcacgggatg gtccccgacg caggggagcc tacggcgaag atccccagcc
      361 ccttgttccc gctcagactg aacttgaccg ggatggcgct gcccgccttc accacgttca
      421 gggtaggtac ggtgtccacc ggcgagaaga agccggtgaa gttgtaaagc accgtgacgt
      481 tgaagctgca cgacgaggta ttgcccgcgt cgtcggtcgc cgacgccgtc acgttggtcg
      541 tgccgacgtt gaagatcgag ccggaggcga tggtcgtcgt cacggggacg ctggcgtcgc
      601 aggaatccgt cgccgtcggc gcgaaactca ccggcatcga gacggccgtg gagttcagcg
      661 ggaggtagac gacgatgtcg gccgggcaac tcatcaccgg cgccgtcgta tcgaccacgt
      721 tcaccgtgcg cgtgaccggc gtggccgcat tgccggccgc gtcggtggcg ttgtaagtga
      781 tcgtgtaggt gcccggaacg ttcacgttaa cggttcccga aggcgtggcc gcgaagttgg
      841 ccgagcagtt gtccgaggcg gtcgccccga gttcggtgta ggacgtgtgg cattccacga
      901 cctgcgggtt ggccccgtcg agcgcgatgg tcggcgccgc gccgtcggcg accgtgatgg
      961 tctgcgaggc gctcgccgtg ttgccgtgcg agtcggtggc cgtgtaggtg cgcgtgatga
     1021 cgagcgggtt ggcgaccgag ccggcgccgc cgttgtcggt ctgcgtcacc ccgactgtcg
     1081 gcgcgccgca gctgtcgtaa ggctggccgc cgggcacgag atcgccgctg gcgtcgcgca
     1141 cgagctggcc ggtgttgtca agcaggggcc cgccagcctg agacgcactg gccgcgggca
     1201 cctcgctcgg gcagacataa gtggcgttcg cgggggcgta gatcaccggc ggcgtcttgt
     1261 ctctgatggt gatgatctgc gtgtcagtgg ccgtgttgcc ggcggcgtcg gtcgccgtgt
     1321 aggtgacggt cgtggccccg atggggaaga agttgcctgc cggcacgccc gtgcgcgcga
     1381 catttacgac gcagttatca ctcacgtcgg gagtccccag ttggctctcg gggacgatca
     1441 ggccgcacac cccggtctgc tcggcctgcg tatccaaaag cacgtcgggc ggcgcgttga
     1501 tggcgggatc cgtctcatcg tccgcgcagg tatccgaggt cacgatgatc ttctgggtgc
     1561 aggtcgagct gaggccgttg ctggtatccg tagccgtcca agtgatgtgc gtcgtcccga
     1621 tggggtaggg cgcgttcagg agttcgttgt cactcctctg acccgacacg gacacgcccg
     1681 acgaagggct ggttgacggc gtccccggat tgacggtcgc ctcggactcg ccggccgggg
     1741 ccgccacgac cacatcgccg gggcaggtga cggtcggagc gcctgccgtg acgaccgtga
     1801 cgtcgaaggt gcaagcgccg gcgccctcgg tcgatgagac gctgacgttc gtcgtgccga
     1861 gggggaagaa ggagccggac ggcgtgttgc tgctaatctc accgcagtcg cccaagccct
     1921 ccgcgctgga gaaggtcacg aaggcgcccg ggttcccgcc ctgcgtggcg ttcgccacga
     1981 cggtgatgtc gtccgggcag ttcagcgcgc acgtcgcggc cgtcacttcg ccggtgatgt
     2041 tcgtggtggc ggtcgattcg ttgtcgccct cgtgcaactc ctcctcctcc tcgaggtcgt
     2101 tcgtgacggt gacgctgtgg ttgacctcgg tgccggccgc cgtgccggtg tccaccgtgt
     2161 agacgaaggc gaactcggcg gtctcgccgg cggccatgct cgcgagcgtg cacgtcgagc
     2221 cgctcggccg ggtgcaggtg aaggcggggc cggactgctg gaccggcggg acggtgtcga
     2281 aggtcgcgtt gttggccggc gtctcggtca ggacaacgtc ggtggcggcg tcggggccgg
     2341 tgttggtgac gaccacgagg taggtgatgc tgtggccggc ggcgtagctc gaaatgcgcc
     2401 ggtcaatggc gtcgtagacg gagaggtcta ccgccttgtt cgcggcgtcg ctcaccgaga
     2461 agaaggacgc cttgcgcagc gagccgtcgc tcgccgtgga gaggtagagc ttccacccgc
     2521 cgcgcgcgcc gacgacgcgc gtggccgtcg gcgaagccgt gctcacgctg tcagtctgga
     2581 ggatggttcc ctcggggctg atccagtaga cgacgcgctc aaagcccagc acccccgaca
     2641 ggttcgtggc cttcgcgcag gccgtctcgc cggcagcgaa gctcgtcttc ggcgtcacgc
     2701 agtcggctgc aaaggattcg acgtcttccg gaccagcctg catgaacaac gccgcgggag
     2761 gcgcggccgc ggccgtggag aaaggactat cggagctgtg cccggcggct ccgttcagtc
     2821 cggcgccggg ggcgctcgtg gagacggaag ccggagaaga agtggagtcg gagccagctt
     2881 ccgcggcggt tgaagccgcg cggcggaagg tggcagagcc ttgatgtggg tagagggcca
     2941 gtgccagcag cccgaccagc accgcgaaag ccacggtacg gaggccggcg acgttagcgc
     3001 gtttcttact tttcgcgctc ggcgtcgttt tttgaaggat ggctgatatt ttgcggtggc
     3061 ggggcagggg ggtaactcgg cgttccatat tccgtctctc cactttttga tttttactta
     3121 agttgtgccg ctctcttcca ccgcgcttta aacttcagta cttcgccggg cgtgatgtcc
     3181 gtctgtcggt cgggagctga aggcatgaga tgggcaagag cttctgaggc gtcatgaaag
     3241 gagtggtact tttcgagtga ctgttagttg cttgctaacc aggtaacagg cttggcgtgg
     3301 aaacaccgga ggcgaagtaa atccgggggc acgtctagca gattcaaacc ctgacagggg
     3361 acgcatcgta tgccaaacgt cgtcccggcg tcaaggaaat aattcataac tctcgcggaa
     3421 tcgtgtcggc agtatgctgg acggcttttc ttgacactct gagaaatgcc tccttatact
     3481 caaaaacttc agacagccgg tagctccagt catccgcaca tgctaattat ctcatccgat
     3541 agactaagga tttcatagcc cggcaagttg tacttagcct ccgacatttt cgggccagga
     3601 tcgaaatcaa gtatgcgtaa attgttcgtt accggcgggg ccggcttcat cggctcggcc
     3661 ttcatcagat tattgctcga cgagtcgccg gactgcctcg tcaccaactt cgacgcgctc
     3721 acctacgccg gcaatctcga caacctctcg ggcctcgacg gcgactcgcg ccaccgcttc
     3781 gtccgcggcg acatcggcga ccgcgccggc gtcctctccg cgctcgaaga gggcacggag
     3841 gcgatagtca acttcgccgc cgagtcgcac gtcgatagga gcatcgcctc ggccgacgag
     3901 ttcattcgca ccaacgtcct gggcacgcag gttctcctcg acgcggcgcg tgagcgcggc
     3961 gtcaaaaaat tcgtgcaggt ctcgaccgac gaggtgatgg ggagcctgcc cgacgacgac
     4021 acggccttct ttaccgaggc gtcgccgttt gagccgaaca gcccctactc ggcctcgaag
     4081 gccgcggccg agcacttcgt ccgcgcggcg cagcacacgc acggcctcga cacagtcgtc
     4141 acgcgctgcg gcaacaacta cgggccgcgc cagttccccg agaaattcct gccgctcatc
     4201 atctcgaacg cgatgcgcga cgaaccgctc ccgctctacg gcgacgggcg ctacacgcgc
     4261 gactggattt acgtcgaaga ccactgccgc gccatcctca tggtgatgca aaagggtcgc
     4321 tcgggcgcgg cctacaacat cggcgcgcgt aacgagcggc gcaacctcga agtcgccgag
     4381 tcggtgctcc gtaaactcgg caagccgctc tcgctcatcc gccacgtcaa agaccgcccc
     4441 ggccacgacc gccgctatgc gattgaccct tccagggtcg agaccgagtt gggctggcga
     4501 ccgcgcgaga cctgggagac gggcctggag aagactgtgc gctggtacgc cgaaaacggc
     4561 gagtgggtcg agcgcgcgcg cagcggcgcc taccgcgagt actacgagca gcagtacggg
     4621 aaaatttaga ggtcagaggt cagaggtcag aggttagtga aaggcggatt ggtttttact
     4681 gacctctaac ctctgacctc tgacctcttc tcttaatgct tatgaaaatt cttgtgacgg
     4741 gcgcaggcgg gatggtcggc cgcgctctgg tcaaacactg cgagggccgc ggcgacgagg
     4801 tgcggccctt cgaccgcgcc gctctggaca tcgccgacga gcgggccgtc tccgagacaa
     4861 tcgggcgcga gcggcccgag gcggtcgtca actgcgcggc gtggactgac gtggacggct
     4921 gcgaagacga ccccgcgcgc gcccacgccg ccaacgcgct cggccccgag catctggccg
     4981 ccgcctgccg ccgcgccggc gccgtgctcg tcaccatctc gaccgactac gtcttcgacg
     5041 gccgcaaaag tggcttctac acgcagcgcg acgaaccgaa cccgcagagc gtctacggcc
     5101 gggccaagct cgaaggcgag cgccgcgcgc agtcggcgtg cgcgcgctcc atcgtggtgc
     5161 ggacgggctg gattttcggc gagggcggaa aaagttttct gagccgcgtc gtcgaaacgg
     5221 ggcggcgcgg cgagcgtctc aaggccatcc aggacgcatg gggaacgccg acctacgcgg
     5281 acgacctcgc cgcgcgcctg cgccggctcg ccgaactcga cctgcccggc gtctaccacg
     5341 tggccaacgc cggcgacggc acgagctact acgagttcac gcgcgccgcg ctcggggcgg
     5401 ccgggggcga cgtgtcgctg atcgagggag tgagcgtaga ctcactctcg cggcccgcgc
     5461 cccggccacg caactcgcgc ctgcgctgcc tgctctccga ggctgtgggg ctgccgccgc
     5521 tccccgactg gcgcgaggcg ctggcgcgct tcgtggccgt cggcgctcgg gagtgagtgg
     5581 cgggcgcccg ctccgggggg cgcgtcgcag gcttttaggt cactcggcgg gccgggcggg
     5641 cgcgtgtaaa aacgcgggcc ggcgttagtt ttccacagct ctcggttttg ccgcgtcgcc
     5701 cgcgtttggt atgatttcgg gcgctgaccc gacggcccga gattctgttc ctattctgaa
     5761 gaggttttaa gcattatgat gtccgaagac aaccggctgc tccctgtcac cacgatagat
     5821 cgcgccctcg accgcccctt gagcgatctg gcggcgagcg ccaagggggg ctacggcgcc
     5881 tcgtcgaacg acccgacgca cctgcgcgac tatctcaatg tcgtcctcaa gcgcaagtgg
     5941 ctcatcctca gcctcgtgct cgtcgtcacg acgctcgtca ccatccagat gtaccgcatg
     6001 ccgactgtct ttgaagcgga gacgacgctc cagatcgacc ccaagcgcga gagcatcctc
     6061 gcccgcaagg accagatcgt catcaacacg ggccgcggcg gcggcgacgc cgtctacgcc
     6121 cagacgcagc tccgtctgct ggaaaaccgc gcgctcgccc gccaggtcat ccgcacgctc
     6181 gacctccaga acaaccccgc cttcctcggc gaccaggcgc aggcgagctt cgtctcgtcg
     6241 ctgcgccgca tcttcctgcg tgagaagaag ggcacgcgct cgacggccga agaccagagc
     6301 atgggcacca tccccgaggt gacgtgggag aactcgcgcg acgagaagct gacggccgag
     6361 gagctgcgcc gcttcgagcc ttatgaggac acgctggccg ccaacctcaa ggtcgagcca
     6421 gagacgggca ccaacctcgt caagctgcgc taccgccaca ccgaccccga gatggcgcag
     6481 aagatcgtca acacgctggc caacattttc gcctacaaca acttcgaccg cgagacgtcc
     6541 ggctccgacc gcaacacccg tgtcctggcc gagaagatcg ccgagctgca actgaagatc
     6601 acgcaactcg aggagcggaa gtacaactac gccaaggcga acaatctgcc cttcgacacc
     6661 aagcagggct ccgatctgga ggccgagcgc ctcgccatcc tgagccgcca ggtgcttgag
     6721 gccgagaact tactgaagga ggccgagacg cagtggaacg aggccaagaa gggcgacccg
     6781 cagacgatcc cgcaggtgca ggacaaccaa cgcgtccaga agctacgcaa ccagctcgac
     6841 gagttggagc agcagaaggc cgagctgatg gaggtctaca cggaagagca cccgcaggtg
     6901 aagaagctcg ataagaagat caccgcgctc agcgcgtcga tgaagaaggc gcccaccgac
     6961 atcgtcagct cgctggagaa acgctaccgc gccgctcagg agaaagtgtc gaagctcaag
     7021 accgactact tccggcaaaa gggcacgacc accacgcaga gtctggcgat gatccagtac
     7081 ggcacgatcc ttcaggacct ggagacgaac aaacaggact tgaacgtgct gcgccagcgc
     7141 ctgcgcgaga tcgagaacgt gaacgttgac cgcatcaaca acgtgagcgt gacgacctac
     7201 agccggcgcg gcaaccccgt cgggccgccg cgcgtgcgga acatcatgct cgcgctcctg
     7261 ctctctctcg gcgccggcgt cggcctcgcc ttcctgctcg actacctcga cgacacgctc
     7321 aagagcgtcg atgacgttga ccgctacctg cacctgccgt cgctcgcgct catcccggcc
     7381 acccgcggcg agagccgctc gcgtctcaag agcggcggcc ccgcgccggc cgagggcgag
     7441 gttacggcgc tggcgctcac cacggacgtg agctcgcccg tcgccgaggc ttaccgccac
     7501 ctgcgcactt cgctcct
//
LOCUS       DASVES010000426         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_663403, whole genome shotgun sequence.
ACCESSION   DASVES010000426 DASVES010000000
VERSION     DASVES010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663403"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..1546)
                     /locus_tag="VF240_17385"
     CDS             complement(473..1546)
                     /locus_tag="VF240_17385"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7177035.1"
                     /translation="MNILGNFFTDDLAIDLGTVNTLIYAPERGVVLNEPSAVAIHKYT
                     GEVLEVGAEAYKMLGREPKDVEVFRPIRGGTINNFEAAEKMLTQFIRRVLGSNHRRSH
                     MVVGVPGSSTPIEQRSVRDAARDAKATRVDLVDEGLAAALGAGLTFEDERAHLVVDIG
                     GGTTNIAIVASGAVVASHSLSVAGNAMDEAIRDYVRSKYEMQLGERAAEGLKKELGAA
                     EAVEDESKQKKMEVVGKQLTDGLAKVVEVSAAEIREALEPVISEIIAGARKAIEESKP
                     DVTADIYETGAILTGGGALLRGMAERLQKELRLHVAVAEDPLAAVAVGAGRLLAAPER
                     LHRAAIRDDVPVWEAQEELVVNW"
     gene            complement(1825..2109)
                     /locus_tag="VF240_17390"
     CDS             complement(1825..2109)
                     /locus_tag="VF240_17390"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX7177036.1"
                     /translation="MRLDLFLRASRLVVRRTLAQELCEAGVVLVNGVAARSSREVRAG
                     DEITLKRVRSILTVRVTEIPTGKQVSKSSAASLYEIVSSQNLESDDPFAV"
     gene            complement(2212..3345)
                     /locus_tag="VF240_17395"
     CDS             complement(2212..3345)
                     /locus_tag="VF240_17395"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7177037.1"
                     /translation="MKFNLLARAVFAALLLVFVAAPVARAQEEEGTAIVLDEPVVQVN
                     NDVVMLSQLRREMKSFKEALKARGMGEPEAEAELAKRQPEIIFSLITEALLIQKGKDI
                     GGLSDRIEAEVNREMLRVCKGQNLPTLERCEEAMRGEGITPEEIRQTLRAQFTKQAVF
                     QQEVDAKIYYGFTDAELQKYHAEHRDKFQSVTLSEIFLSTAGRKPEEVGAKAAQLVAQ
                     ARAGGDFGALAEANSEREVNGERVSKKTKGRIEDNGKPRWFLVSDLASVSQKIADAIK
                     PLKANQITDAVQTDEGFVIYKVHERDDVYNENQVRNEMLRDGGRLDKERVAYIASLRK
                     EAYIKPADNYKEVLQPFLDKDQPATVSKDSGDKNSKDKKPQKQ"
     gene            complement(3455..>3738)
                     /locus_tag="VF240_17400"
     CDS             complement(3455..>3738)
                     /locus_tag="VF240_17400"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX7177038.1"
                     /translation="PPEPVESLFAYARLRRMAEAVGVVSIDRTPTGLALKLRENARVS
                     PERLLALVGGATGASFSPSGVLRLEAIEGEGGSLIEVAHRTLLELRAAD"
BASE COUNT          650 a         1189 c         1141 g          758 t
ORIGIN      
        1 gactttcgca gaggcgtctg cgcgaggcca ttcgcgccgg gcaactgagg gcgcagatca
       61 tcggccgcgg ctggcgcatc aagcgcaccg acctcgaaga ctacgtgcgc gacctctgac
      121 attaagagtc tgtttccaaa cctcaacgcg ggtgataact gtaggggcag ggcttgtccg
      181 cggcccgtta ccaacggaag caattaacat ggatggacag gataatcagg ataagagatt
      241 tgaaatttga aatctcaaat ctgaaatcag cgctttcttt atcctaccca tcctgcccat
      301 ccatgttaat tcttctgtag gcacgggccg cggacttgcc ctgcccctac agttttgcca
      361 cgcgctttga gtgttaagta tgaggcgcaa cagaaaaggg agggccgaag ccctcccctg
      421 tcgtatcgtg cggtcgccag tgtcgctggc cgagcgcgtc gagttacaag tcctaccagt
      481 tgacgaccaa ttcctcctga gcctcccaga cgggcacgtc gtcgcggatg gccgcgcggt
      541 gcagccgctc gggcgcggcg agcagacgcc cggcgccgac ggcgaccgcc gccagcgggt
      601 cttccgccac ggccacatgc aggcgcagct ccttctgcaa gcgttccgcc atcccgcgca
      661 aaagcgcccc gccgcccgtc aggatcgctc ccgtctcgta gatgtcggcc gtcacatcgg
      721 gcttcgactc ctcgatggcc ttgcgcgcac cagcgatgat ttccgagatc accggctcca
      781 gcgcctcgcg tatctccgcc gcggagactt cgaccacctt cgccagcccg tcggtcaact
      841 gcttgccgac aacttccatc ttcttctgct tggactcgtc ttcgacagct tcggccgcgc
      901 cgagttcctt cttcagacct tcggcggcgc gctcgcctaa ttgcatctcg tacttcgagc
      961 gcacgtagtc gcggatggcc tcgtccatcg cgttgccggc gacgctcagg ctgtgcgagg
     1021 cgacgacggc gcccgaggcg acgatggcga tgttggtggt gccgccgccg atgtccacga
     1081 cgaggtgcgc gcgctcgtcc tcgaaggtga gacccgcgcc gagggcggcg gccagtccct
     1141 cgtcaaccag atcgacccgc gtggccttgg cgtcgcgggc ggcgtcgcgc accgaacgct
     1201 gctcgatggg agtggaggag ccgggcacgc ccactaccat gtggctgcgt cgatggttgc
     1261 tgccgagcac gcggcggatg aactgcgtga gcatcttctc ggccgcctcg aagttgttga
     1321 tggtgccgcc gcggatgggg cggaagacct ccacgtcctt cggctcgcgg ccgagcatct
     1381 tgtaagcctc ggcgccgact tcgagcacct cgcccgtgta cttgtggatg gccaccgccg
     1441 acggctcgtt gaggacgacg ccgcgctcgg gcgcgtagat gagcgtattg accgtgccga
     1501 ggtcgatggc cagatcgtcc gtaaagaagt ttcccagaat gttcataaag ctctccggtg
     1561 accgaaaaac cgatgcgagg agttatcgca cacaggattc aacgaacagg tggcaaaccg
     1621 cgtaccattt ggcgcaggtg tgtccggccg cgggatttaa ggcagtgagg tggggggcgc
     1681 gtttcgattc gagacaacca ctcgcacatt ccgccgcctg tcgtgggcgg aagacggacg
     1741 ccgttaattg cggcctacta cggcaaatac tgcccatacg aatgggcgac ggcaatcact
     1801 tgcgatttgc tcgtgagtag atattcaaac ggcgaatggg tcgtctgatt ccaggttttg
     1861 agaactgacg atttcgtaga gtgaggcggc gctggatttg gagacctgct tgccggtggg
     1921 aatttctgtg acccggacgg tcagaatgct tcgcacacgc ttcaaagtga tttcatcgcc
     1981 ggcgcgcacc tcgcgcgaag aacgcgccgc cacgccgttc accagcacca ccccggcctc
     2041 gcacaactcc tgcgccagcg tgcgccgcac cacgagccgg ctcgcccgaa ggaagagatc
     2101 aagacgcata aggatggtga taagtgatga gtgataagtg ataagtgatg agtgataaga
     2161 aagaatagtg tcgtcttctt ctctttctta tcacttatca ctcatcacct atcactgttt
     2221 ttgaggtttc ttatccttgg aatttttgtc gccggagtcc ttgctcaccg tggccggctg
     2281 atccttgtcg aggaacggct ggaggacttc cttgtaatta tccgcgggct tgatgtaggc
     2341 ctccttgcgg aggctggcga tgtaggcgac gcgctctttg tcgaggcgac cgccgtcgcg
     2401 cagcatctcg ttacgcacct gattctcgtt gtagacgtcg tcgcgctcgt gaactttgta
     2461 gatgacgaag ccctcgtcgg tctggacggc gtcggtgatc tggttggcct tgagcggctt
     2521 gatggcgtcg gcgatcttct ggctgacgga cgccaggtcg gagacgagga accagcgcgg
     2581 cttaccattg tcttcgatgc ggcctttggt cttcttcgac acgcgctcac cgttcacctc
     2641 gcgctcggag ttggcctcgg ccaacgcgcc gaagtcgccg ccggcgcgcg cctgcgcgac
     2701 gagctgcgcg gccttggcgc ccacctcctc gggcttgcgc ccggccgtgc tcaggaaaat
     2761 ctccgagagc gtcacgctct gaaacttgtc gcggtgctcg gcgtgatact tctgcaactc
     2821 ggcgtccgtg aagccgtagt aaatcttcgc gtccacctcc tgctggaaga cggcctgctt
     2881 ggtgaactgc gcgcgcagcg tctggcgaat ctcctcgggc gtgatgccct cgcccctcat
     2941 cgcctcctcg caacgctcca gcgtcgggag gttctgtccc ttgcacacgc gcagcatctc
     3001 gcgattgacc tcggcctcga tgcggtcgct taagccaccg atgtctttgc ccttctggat
     3061 caggagcgcc tcggtgatga ggctgaagat gatctcgggc tggcgcttgg cgagttcggc
     3121 ctcggcctcc ggctcgccca tgccgcgcgc cttgagcgcc tccttgaacg acttcatctc
     3181 gcgtctgagc tgcgagagca tgaccacgtc gttgttgacc tgcacgaccg gctcgtcgag
     3241 gacgatggcg gtgccctcct cctcctgcgc gcgcgcgacg ggggcggcga cgaacaccag
     3301 aagcaacgcg gcgaagacgg cgcgcgccag taagttaaat ttcacagctt ttaaactcct
     3361 cacagtttta tttcgtgtgc cgggattgat gattttacaa gccgggtggc gctatcagac
     3421 ggcaagcctt ccggtcgcga acggaacggg cattctaatc cgccgcgcgc aactccagca
     3481 acgtgcggtg cgcgacctcg atgagcgagc cgccctcccc ttcaattgcc tccaaccgca
     3541 acacgccgct tggcgagaag ctggcgcccg ttgcgccgcc cacgagtgcg agcagccgct
     3601 cgggcgagac gcgcgcgttc tcccttagct tcagggccag ccccgtcggc gtccggtcga
     3661 tggagacgac cccaacggcc tcggccattc gtcgcagacg cgcgtaagcg aacaggctct
     3721 cgaccggctc gggcggac
//
LOCUS       DASVES010000427        24559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_430754, whole genome shotgun sequence.
ACCESSION   DASVES010000427 DASVES010000000
VERSION     DASVES010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 24559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24559
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430754"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1004
                     /locus_tag="VF240_17405"
     CDS             249..1004
                     /locus_tag="VF240_17405"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX7177039.1"
                     /translation="MNREAMYAIMQGAGTTDYELYLDTPALLACQKDYQDLCNQDELQ
                     FQIVHQIEELWMKLIGYTLLDIDECIQAQRTNRVITLFRRVHRIQELMNMQFSLLETM
                     SPKDYQGIRATLGNGSGQESPGFRSLLRMSRQLWQSFETHYLDAHGLTVEKIYDSEYS
                     HSDAYVVAEALAEYDSLFRQFRFQHIQLIRRSIGLGARSLKGRPVEMLEAGIRYQFFP
                     ELWAIRDQMTDNWGATYGVVRDSLGCPHAHAAA"
     gene            991..2112
                     /locus_tag="VF240_17410"
     CDS             991..2112
                     /locus_tag="VF240_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7177040.1"
                     /translation="MQPHKHDFLLPDGTYLLSHSVGCLPRAAREAADQFFELWGTRGG
                     EAWDGWLASIGDFQQSLATLLNGEAAEFCPQANISSALAKILSSLPRRKGKNKLLLSE
                     LDFPSAGFALAQAERAGYTIEFIPADSDTSSPDCWQQHITPDTQLVLITHVLYGNSRL
                     NPVAEIIRCAQQHEVLTLVDVAQSVGVVPIDLRAWNADFVVGSSIKWLCGGPGAGFLW
                     ANPAGVERFEPSDVGWFSHEDPFEFRIQSFRYAPDARRFWGGTPSVLPYVIARASIDV
                     VNRVGVATVREHNQTLTDRLVRAALERGLTLRTPHDPARRGGTVCVAFRDSQRVYQQL
                     KEERIFVDVRPAYGVRFSPHIYNDAEEIDHAIASFETAA"
     gene            2148..3203
                     /locus_tag="VF240_17415"
     CDS             2148..3203
                     /locus_tag="VF240_17415"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7177041.1"
                     /translation="MDELLDPAAGTPPALLLRRLIGSYRITHAIKAAAELGVTDHLGE
                     RPQSAGELAARLHVNPQALYRLLRTLVSIGLLADAGDGLFTLTPVGACLRRDAPDGMR
                     AWALCEGAEYYQSAWMNLPHAIQTGETAFEYTHGMPFYQYLARHPETGHGFSQAMLDY
                     ARLIATAVVAGYDFARVRRVVDIGGSHGHLLSAILNANPIVSGVLFDRPAVIERAEER
                     IRGTELAGRAELVAGDFFESLPAGGDLYILSRILMDYDDDASVRLLRNCHRAMTPGGR
                     VLIVQLLMPEQGGDAARERLFEAAVSDLNMLVLTGGRERTEGEYRTLLGLAGFRLARV
                     VPSQALVSLIEGERIET"
     gene            3229..4008
                     /locus_tag="VF240_17420"
     CDS             3229..4008
                     /locus_tag="VF240_17420"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7177042.1"
                     /translation="MTNVLTQLVDELNAGRVRVVDLTLPLDSETPVIGLPPMFAASPC
                     FSMEVISRYDERGPVWYWNTLHVGEHTGTHFDAPVHWATGKDLPDNRCDTIPARRFVG
                     PACVIDVTAEVAADADFLLTADHVLAWEAEHGHIPAGAWLLLRTDWSKRTDPAAFLNV
                     AEDGPHSPGFDPSASKLLAYERDILGVGVETIGTDAGQAGRFDPPFPNHSIMHGAGKF
                     GLASLCNLDQLSATGAVVIAAPLKIVDGSGSPLRVIALSPD"
     gene            4288..4854
                     /gene="mug"
                     /locus_tag="VF240_17425"
     CDS             4288..4854
                     /gene="mug"
                     /locus_tag="VF240_17425"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEX7177043.1"
                     /translation="MAPHPRRPTKEELLAAAGKTVPDLIAPRLRVLFCGINPGLYSGA
                     VGCHFARPGNRFWPALYAAGFTERQLSPFEERELLKSGYGITNVVERATATAAELSAH
                     EFVEGGRRLAAKVERYRPRCLAVLGIGAYRAAFALPKARLGRQDETIGDAVVWVLPNP
                     SGLNAHYQPKDLARVFGELRSAVVGDRR"
     gene            complement(5105..5461)
                     /locus_tag="VF240_17430"
     CDS             complement(5105..5461)
                     /locus_tag="VF240_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177044.1"
                     /translation="MEKQAYVVGNRVEKLCAICGVERGHVVASVTKQGRISRVDCPKC
                     GTRSTFKSGVKLVGTGDAPEPGALYDRTRSYRKGQLITHPSFGAGEVTAVIEPQKIDV
                     LFADRVRRIIHETSPV"
     gene            complement(5582..5917)
                     /locus_tag="VF240_17435"
     CDS             complement(5582..5917)
                     /locus_tag="VF240_17435"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7177045.1"
                     /translation="MSMKLYVGNLSFQTSSEDLQQLFAQAGTVESAAVVEDRDTGRSR
                     GFGFIEMSSKEEGAAAIAKFNGYDLGGRALNVNEAKPREDRGGGGGGRNGGGGGRGGY
                     GGGGGGNRY"
     gene            6344..7231
                     /locus_tag="VF240_17440"
     CDS             6344..7231
                     /locus_tag="VF240_17440"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7177046.1"
                     /translation="MERLTEIKRERESINWTTTIFVSIFHLGAIAALFMFSWEALAVS
                     VFLLWLAGSPGIGMGFHRLLTHRGFKTPKWVEYLLSICGTLTLEGGHIDWVVTHRIHH
                     AHTEVPGKDPHTPREGGWWSHMGWILRGTAQQHGEAVMMRYAPDLMRDRFHVLLNKVY
                     WLPLVVVGAALLYFGGWSVFMWGVFGRVVFGWHSTWFVNSATHLWGKRRFVTTDDSTN
                     SWWVALLTFGEGWHNNHHAHPRAARHGLMWYEVDMNWYGIRALELMGLATDIKLISPA
                     AIRAAAAAAAEPELLRRAA"
     gene            7325..8245
                     /locus_tag="VF240_17445"
     CDS             7325..8245
                     /locus_tag="VF240_17445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7177047.1"
                     /translation="MPSYNAAMRIGGRRKTVIFFLTFGILLVALALALNVGWILLNWR
                     QVALLVLGIVFFTLIITGLVLNTTFLIREIRRNEQHDAFINSVTHELKTPIASIRLYL
                     ETLKTRDVNEAQRKEFYDIMLADSDRLLQTVEQVLRAGRASHKQRLANVSVIDLGRVV
                     RECLDLTRVRYNLNPDALRYVESMGDARARVAGDEDELRAAVSNLLENAVKYSDKEVH
                     ISVEVAALDERRLAVRVADRGVGIPSSQLKRIFKRFYRVQGGFMKRFKGTGLGLFIVR
                     SIVEKHGGRVFAESAGLGQGSTFTIHLPRA"
     gene            8252..9055
                     /locus_tag="VF240_17450"
     CDS             8252..9055
                     /locus_tag="VF240_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7177048.1"
                     /translation="MSAVLIVEDEQHLADGLRFNLEAEGYTVETVADGEAALTLLIDR
                     REHFDAVVLDVMLPGKDGFTVASELRQAGRFVPVLMLTARGRPEDVLRGFESGADDYL
                     PKPFELAILIARLNGLLRRRGWFQLDPDISSPPDGPAAPSGASQSESLKDGKRAADLV
                     GELFVFDGKTIDFGALELRTRDQTMRLTLMEARLLRHLIGHAGRVVSRKSLLEDVWGL
                     QEDTDTRAIDNFIVRLRKYIEDEPARPRHLLTVRGVGYRFVAEPGRPAA"
     gene            9105..10271
                     /locus_tag="VF240_17455"
     CDS             9105..10271
                     /locus_tag="VF240_17455"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX7177049.1"
                     /translation="MFKSKSASFVVAALLSVVGARAACAQALYELPENVETRWASPEN
                     PRGEKGRGAQAAGGRKGSPTIAIRAGASAVLAEAQNTSGMVRRIWMTIPERSPQMLRG
                     LRLDMFWDGAARAAVSAPLGDFFGVGLGQMAQFESALFSNPEGRSFNCVVPMPFKTGM
                     KIVMTNESGRDLPELFYDVDYTLGDRHPVGTLYFHAHWRRENPTTLQRDYEILPSVAG
                     RGRYLGANIGVIVDRDTYFNTWWGEGEVKIYLDGDAQFPTLAGTGAEDYIGTAWGQGR
                     FAHLYQGSPVADEQNMRWCFYRYHVVDPVYFRRDVRVTMQQIGYLADHSRGGLARTAR
                     RLYRAGPGLVEKDIKEDGKFERTDDWSSCAYFYLDRPENNLPQLDAAEKRMAGL"
     gene            10285..11868
                     /locus_tag="VF240_17460"
     CDS             10285..11868
                     /locus_tag="VF240_17460"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177050.1"
                     /translation="MRFKRVRKLGRAASASVFILLLWSCAAQTAAQEPNYARLERAAE
                     MIRRGQLAQAETELNTVLRARPEEANALNLLGVVRAQQKRFEEAERLFLRAVKSSPTL
                     VGAFVNLGQLYISTGDEERALRAFDAAAALAPDHPEVNYHLAAIREGRREFARALEHL
                     GKIPGELRGLEHLQLVVRCLLGLGRADEAAGLVAPLRSAQNTAATVPPEDAAGFAALF
                     AAHARTDVALEILGAARGRAPDSFAVLYNLGATHLQRRELERAEEFYRRALAARPDDG
                     ATLRALAGVERARGNLEKSLGYVERALRLDPDSRALLYDYGWTALNLDRIPQALPALE
                     RLHRAHPQESAYIYALAVARFYNNEDAQALALLRRYIGLNPRDPRGHYVLGVMLQLMM
                     RRADARVALAESFDLTPTADAAHYLGLVAYDEADLAQAEHWLRRAVELEPSHAPARAA
                     LGMTYARRKDLAAARAELERAAALDPQDLTAAYQLGLLYTRLGERERARAMLAVADRL
                     RAEQRQTGRAGLRLAEPPK"
     gene            12242..15982
                     /locus_tag="VF240_17465"
     CDS             12242..15982
                     /locus_tag="VF240_17465"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7177051.1"
                     /translation="MKVNRLVVLALLALCSTWGVSAHAQSDRGTITGTVTDQSGAVVA
                     AAKVTATATDTGEVRETTTSGEGSYTFAEVKAAPYRISVEAQGFKTATTEDVKVAVQV
                     TRTVDITLEVGVVGDTVTVTAEDTPVIQTESAVRQTNVNERQVKELPLAVAAESGGRT
                     PLAFIFLDSNVSAGSGTTGRGTDASNFRVSGGQGLGTEILIDGASTRRAQNGTFFSEV
                     APGPNAFQEFTISTSSYSAEFGSSSGGIVNFTLKSGGNDFHGELYELHRNDAFNANTY
                     VNNALGLARPRDHQNNFGGNIGGPIYLPRFGEGGPAYWSGKNRAFFFFNYEGFRFARQ
                     ENTLVSVPTARMRAGDFSELLTDPYVLSSLGGPVQIYDPTQAPGSRTAIPGNRLDLYR
                     GGAVLDPAGVALANLFPAPTRAGVFRNYDARSLSPTTMDNYVGKTDFMLSDRQRLGVS
                     YSYRKLDSIKGFGGRPAFTRLPPPFTQQEVWNQAFKSHFARVQHDITLSPTVINHLNL
                     GFTRYDVANMNFGEGIQPSSFGVPAGATANFALPRVGFPGYGDFITGGDPRSYIDAGS
                     TFFTDHLYDNTVQISDALTWIKGRHTMKFGGDVRIQQFNVTQQIDPGGSFNFRHDQTA
                     ENVVVGTNEEGNPITQVQGGWPIASLVTGATEFSFQTVTAVDPGWRYFYPAVFFTDDI
                     KLTQRLTLNVGARYEISYPRTESKGRLRGFDPDAINPAVGIRGALVGAAGQGGVSAEH
                     EGLARPDYSNFGPRVGFAYALNDKTVARGGIGIYYAPILYGFEGGNTIEASLGYRNSA
                     TPNDGCPRCRRPDFGSLQARYFLRNLPASGPLAPTADPNDQFVNQGIAVDYFNKDYRT
                     GRTVQYSLDVQRSLPSKFAASIGYIGHRATRLRSNFERLNAVPLDALKLGYPILNTSL
                     ADARNNAAFVTYAQSVSVPLPAVPYAGFNGTVAQALRPFPQYGRIRSQLESNGQSWYN
                     AMQAKLDRRYSNGIQFGASYTFSKLITDASEDLFGGSPLGEILQNPYDRRQLRSVSAN
                     NPYHVFVFNYILELPFGKGRRFANRGGAVDKILGGWQVSGIHRYQSGLPLVVRDTEPG
                     RAGFLSQVGFEGALRPNLSGQPILTGNPETGIRFQLVNPGAFSAPPNFQAPPTDNVTT
                     AAYRNYYANPLAFFGAAPPVIDSARYLPFFSENVSLLKKTHITETTTLEFRAEVFNVF
                     NRHRYFGPANDLRFGDFGNSGAIDNRDVYAPREIQLGLRFIF"
     gene            16068..17792
                     /locus_tag="VF240_17470"
     CDS             16068..17792
                     /locus_tag="VF240_17470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177052.1"
                     /translation="MARLRAHSTHALICAAALCVSAPHAAATTGAAARARTPAQSPQR
                     KGGRSPRKSANEAARLVGEGVAALERDERARARELFERALQIEPSNSDAHTYLGALAD
                     QSGDLAAAERHFAAAARAAPASAPARNNHGAALMRLGRTREAAREFAAALRLDAGHAG
                     ALVNLAQIRFAAGTPDGLRAAYELLERARAAAPGDAEIARSLLVTALRLGERAAVARH
                     FRDYTSALARASAGGGDAAAVGTGASALPSARAELGAALLEGGFAEEAAEELAAGAAG
                     EPSKAEYIVMLARSHMARKDVKAAGRALEGALARGLEAAPIYAALAEVYEASGHVENA
                     IPAMRLAIERDPRNESYRFRYGMLLTDTHAPAAAVVRLEEALKEFPRSSRLWFALGVA
                     QAALNRADEAAKSFERARDLDPKFAPALAYLGMTHDVRGRFAEAVELYERALALDDRL
                     AAAHYLAAEAILKQAPADETRAEPHLKRAVELDPRFAPARVSLAKIYMRGERFEETVA
                     QLQSAVAADPALAAAHYQLGRALLRLKRNSEGQAALAEFKRLSEGQREQAREGPREIM
                     RRLANVRF"
     gene            17811..18560
                     /locus_tag="VF240_17475"
     CDS             17811..18560
                     /locus_tag="VF240_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177053.1"
                     /translation="MSRHSVRTCALLFACVFLGRTTVSAQQASATPSPSPHSQPQQPP
                     PPRAFDEWADGFDGDKLDESKWERFTFEGGGGGKLEVKEGELRLRSGGGTRAGVRSRE
                     EFAGEQFSVEARVAKVGPAYPDPNQKGEPIGFAALTILFDGSGRNRVEWVLTSAGRFE
                     AWAVVDGRAEQLDDRNLGTKMKSPVIAIIRRGEEFLFVLNGPDSPPQDARVGLRKTVK
                     NLPRRFRVMLYGFSSSENNWDAVRVVTPRRQ"
     gene            18599..19798
                     /locus_tag="VF240_17480"
     CDS             18599..19798
                     /locus_tag="VF240_17480"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX7177054.1"
                     /translation="MKPFGPRQSARAAGLLAALALCAAAAAGPSVRAQRAPRAGAAAS
                     SATYANPVFDEDFPDPTVIRASDGWFYAYATTTNIGGRVYHIQAARSRDLVSWERLPD
                     VLPAKPAWSSQHDLLWAPDVHQRGRTFYLYFSNRLNDDLAARFKRENNAESSKEDVFC
                     LGVATARLPIGPFTDSGRPLKCGLSFVNIDPMAFDDPRTGRKYLFWGSGFQPIRVQEL
                     AADRLSFKAGSRPVELVAASKDLPYQTLIEGAWVVYRGGFYYLFYSGENCCHGPLQQI
                     KYAVLVARARQVEGPYQTLAQARGGRSSAILERSDAWIAPGHNSIVEDDAGQSWIVYH
                     AIDVKRPYMTATLKDDRNVRRVMLLDRLAYRGGWPFVEGGGKPSRGPRPAPFVRRGRP
                     PPRRGRE"
     gene            19952..21829
                     /locus_tag="VF240_17485"
     CDS             19952..21829
                     /locus_tag="VF240_17485"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7177055.1"
                     /translation="MMTRSENISAAKWPTLLLLAASLMLACAPPARARVAQASSEAPR
                     AEHPRPDFVRADWQTLNGRWEFEFDDADRGLAERWYDLAAGKGFSKTILVPFAFQTRL
                     SGVGVTDFHDVVWYRRAVQAPDSFRRPGRRILLNFGAVDYEATVWVNGEQAGFHRGGH
                     VSFSLDVTDLLKAGDNSVVLRVFDPSADRTVPRGKQFWRPKSEGIWYTRTTGIWQPVW
                     MESAGDTHVRRLRVTPDVDNSRVSVEAVLNKTAPGLRLRTVVKLKGEVHAQSEVACAD
                     ARPLAVHKLNEQRLWSPWEPTLYDLTVELVGAGGQVVDRVESYFGQRKVSTHDGRVYL
                     NNNPYYLRLILDQGYWPESLLTPPTDEAMRRDIELSKAMGFNGARKHQKAEDPRWLYW
                     ADRLGYFVWGEMANAQDYSEEYAARFADEWQQIVARDFNHPSIIVWVPINESWGVPDI
                     FTSRAQQDHARTMYHLLRSIDPTRLVIDNDGWEHTDETDLFALHDYARTGEELAEKYK
                     ILETDTSRIPRNGRDALALGYKYNGSPFVLTEFGGIAYRVGAPKAANEWGYSGIEPTK
                     EAMLKRLDGLVKAVVANKAWAGFCYTQLTDVEQEINGLLTYDRRPKAAPEEFKRIFEQ
                     R"
     gene            21934..23103
                     /locus_tag="VF240_17490"
     CDS             21934..23103
                     /locus_tag="VF240_17490"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX7177056.1"
                     /translation="MIDTGMLRRAFRRLSAEEPRLFRAPGRVNLIGEHTDYNDGFVLP
                     MAIDRGTVVAAAARPDRKVRAYSLNLNQTVEFDLDGPGAGRRGVWLDYVEGVARELEK
                     RGTRLRGADLALDSDVPVGAGLSSSAALEISVGLALASVAGAEIDGVTLALAGQQAEH
                     NYVGTQCGIMDQFVAAMGRRRTALLIDCRTLATIHIPLDSASAAIVICDTRVKHTLAN
                     SEYNTRRAECERGVEILKGLLPQEVRALRDVSVEDFERHQVSLPEPVRRRVRHVVTEN
                     ARTLLAAEALRAGDLEGVGRLMALSHRSLRDDYEVSCRELDTLVRVAETTEGVIGARM
                     TGAGFGGCTVNLVRHGALENFRKTLSLECNRAGQHAPAVYVVESSDGAKEVTTDK"
     gene            23193..24206
                     /locus_tag="VF240_17495"
     CDS             23193..24206
                     /locus_tag="VF240_17495"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7177057.1"
                     /translation="MSSIRDVARTAGVSTATVSHVVNNTRFVSEEVRARVLAAVEQCG
                     YYPNAHARGLASGRSRILGLVISDISNPFFPELVKAIETAAFEQGYDVLLSNTNYDPD
                     RTSHYVRRLIERKVAGVALMTSELDAALIDELARRDVCVVFLDLGEPGRHMSNLRVDY
                     EAGIEEAVRHLVRLGHRRVTFVSGPAHLRSAERRLEAFRRSLARHLPGAPVEVYDGDF
                     RLDGGRRAVRRMHADGELPTAVLAANDMMALGAMAELRALGVEVPRDVSVVGFDNIEF
                     AALSQPALTTVCLPRSELGRRAVEALLETIGHTEQRGVEIEIPTHLVVRGSTARARGV
                     END"
BASE COUNT         4156 a         8603 c         7874 g         3926 t
ORIGIN      
        1 ccggcggcgg ccccctgggg tcgccgttca gcttgtgtgg ggaaagggca cagagcgcac
       61 agaggaagca ctttgtcttt ctctgtgaac tctgtgccct ttgcgccctc tgtgttgaaa
      121 tgaactcgcc cgacgctcaa ccctgtttga aatttgctct tacttctcct gatccctctc
      181 gcccctgctt tgcgcgttag tctatgggac gctaaaaccg gcgcgttgaa ccacaggagg
      241 aggatgctat gaaccgagaa gcgatgtatg ccatcatgca aggcgcggga accacggatt
      301 acgagctgta cctcgacacg ccggcgctgc tcgcgtgcca gaaggactat caggatttgt
      361 gcaatcagga tgagcttcag ttccagatcg tccaccagat agaggaactg tggatgaagc
      421 tgatcggtta tacgctcctc gacattgatg agtgcattca ggcgcagcgc acgaatcgcg
      481 tcatcacgtt gttccgacgt gtccaccgca tacaggagct gatgaacatg cagttctcgc
      541 tgctggagac gatgtcgcca aaggattatc agggcatccg cgcgacgctc ggcaacggga
      601 gcggtcagga atcacccgga ttccgcagcc tgctgagaat gagccggcaa ctctggcagt
      661 cgttcgaaac ccactacctc gacgcgcacg ggctgacggt cgaaaaaatc tacgactcgg
      721 aatacagcca tagtgacgcc tacgtggtcg ccgaggcgct ggccgagtac gactcactgt
      781 ttcgccagtt ccggttccag cacatacagc tcattcggcg ctcgattggt ctgggcgcac
      841 gctccctcaa agggcgtccg gtcgaaatgc tggaggcggg catccgctac caattcttcc
      901 ccgagctgtg ggcgatcaga gaccagatga ctgacaactg gggcgcgacc tacggcgtcg
      961 tccgcgactc gctcgggtgc ccacacgctc atgcagccgc ataagcacga cttcctgctg
     1021 cccgacggga cctacctgct cagccattcc gtcggctgct tgccgcgggc ggcgagggag
     1081 gctgccgacc agttcttcga gttgtggggg acgcgagggg gcgaggcatg ggacggctgg
     1141 ctggcgtcaa tcggcgactt tcaacagtcg ctggcgacgt tgctgaacgg cgaggccgcc
     1201 gagttttgtc cgcaggccaa catctcttcc gcgctggcga aaatcctgtc gtcgctccca
     1261 cggcgcaaag gtaagaataa gcttctgctc tccgaactcg atttcccttc ggcgggcttc
     1321 gcgctggcgc aggcggaacg agcgggctac accatcgagt tcatcccggc ggacagcgat
     1381 acgagttcgc ctgattgctg gcagcaacac ataacacccg acacacaact cgtgctgatc
     1441 acgcacgtct tatacggtaa ctcccggctg aacccggtcg cggagatcat ccgctgcgca
     1501 caacaacacg aggtcttgac gctggttgat gtcgcccagt cggtgggcgt ggtgccgatt
     1561 gacctgcgcg cgtggaacgc ggatttcgtg gtcgggtcgt ctatcaagtg gctgtgcggc
     1621 ggccccggcg ctggctttct ctgggcgaac cctgccggcg tggagcgttt cgagccgtcg
     1681 gacgtgggct ggttttcgca cgaagaccca tttgagttcc gtattcaaag cttccggtac
     1741 gcccccgacg cgcggcgctt ctggggcggg acgccgtcgg tcttgcccta tgtgatcgca
     1801 cgcgcgagca tcgatgtcgt caaccgtgtc ggcgtggcga ccgtgcggga gcacaaccag
     1861 acgctcaccg accgtctcgt gcgcgcggcg cttgagcgtg gtctgacgct tcgcacgccg
     1921 cacgacccgg cgcggcgtgg gggaacggtt tgcgtcgcct tccgggactc gcagcgggtc
     1981 taccagcaac taaaagaaga gagaattttc gtagacgtgc gcccggcata cggcgtgcga
     2041 ttctcgcccc acatctacaa cgacgcggaa gagatcgatc atgctatcgc ctccttcgag
     2101 acggcggcgt gacggaagat gagcagccga caggagggtg cgtcaggatg gatgagttgc
     2161 tcgacccggc agcgggtacg ccgccggcgc tgcttctccg acgactgatc ggcagttatc
     2221 ggatcacgca cgcgatcaag gcggcggccg agcttggtgt tacagaccac ctgggcgaga
     2281 gaccgcagag cgccggcgag ctggccgccc ggctgcatgt gaatccgcag gcgctctatc
     2341 gcctgctgcg gacactggtg agcatcggcc tcctcgccga cgcgggtgac ggcctcttta
     2401 ccctcacgcc ggtcggcgcg tgcctgcgac gggacgcccc cgacgggatg cgcgcctggg
     2461 cgctgtgcga aggggccgag tattaccaga gcgcatggat gaaccttccg cacgcgatac
     2521 agaccggcga gaccgccttc gagtacacgc acggcatgcc gttctatcag tacctcgccc
     2581 ggcatcccga gacggggcac ggcttttcgc aggcgatgct cgattacgca cggctgatcg
     2641 ccacggccgt ggtggccggc tacgatttcg cgagggttcg ccgggtcgtg gacatcgggg
     2701 gcagtcacgg acacttactg tcggcgatct taaacgcgaa tccaatagtc tccggcgtgc
     2761 tctttgacag accggcggtg atcgagcggg cggaggaacg tatccggggg actgaactcg
     2821 cgggtcgcgc cgagctggtc gcgggcgact tctttgagtc gctgccggcg ggcggcgacc
     2881 tctacattct ttcacgcatc ctcatggact acgacgacga cgcttcggtg cgcctgctgc
     2941 gaaactgcca ccgtgcgatg accccgggcg ggcgcgtgct gatcgtccaa ctgttgatgc
     3001 cggaacaggg cggcgacgcg gcgcgagagc ggctgtttga ggccgccgtc tctgacctca
     3061 acatgctggt gctgacgggc ggccgtgagc gcaccgaagg cgagtatcga acgctgctcg
     3121 ggctggcggg tttcaggttg gcgagagtcg ttccctcgca ggcgctggtc agtctcatcg
     3181 aaggcgaaag gatagagaca tagtgattag gagggagaag ctcacgtcat gacgaatgta
     3241 ctcacacagt tggtagacga actcaacgcc ggacgggtgc gcgtcgttga cctgacactg
     3301 ccgctggatt cggagacgcc ggtgatcggc ctgccgccga tgtttgccgc ctcgccgtgt
     3361 ttttcgatgg aagtcatctc gcgttatgac gagcgggggc cggtctggta ctggaacaca
     3421 ctgcacgtcg gcgagcacac cggcacgcac ttcgacgcgc ccgtccactg ggcgaccggc
     3481 aaagacctgc cggataatcg ctgcgacacg atcccggcgc ggcgcttcgt cggccccgcc
     3541 tgcgtgatcg atgtgacggc cgaggtggcg gccgacgctg attttctgct taccgcggat
     3601 cacgttctgg cgtgggaggc tgagcacgga catatcccgg cgggcgcgtg gctcctgctc
     3661 agaaccgatt ggagcaaacg cacggacccg gcggctttcc tcaacgtcgc cgaggacggg
     3721 ccgcatagcc ccggcttcga tccgtcagcc tcgaagctgc tcgcctacga gcgcgatatc
     3781 ctgggcgtcg gcgtggaaac catcggcacc gatgcgggac aggcggggcg gttcgacccg
     3841 cccttcccca accatagcat catgcacggc gcggggaagt tcggtctggc gagcctctgc
     3901 aatctcgatc agttgagcgc gaccggggca gtggtgattg cggcgccgct caagatcgtg
     3961 gacggcagcg gaagtccact gcgggtgatc gcgcttagtc cggactaagc aggaggcaat
     4021 tgcagtgata cgcaaggccc tggtaagcac cgccgcgtgc aggtgtgatg gccgtcactg
     4081 tggtgtcttc cggcataaac aataaatctt gaccatggcc tgtgtcgcat gctacatagc
     4141 gtgcccgctc gttgccgacc agtttttcag gcgtcaaccg cgggccggtt aatttgaacc
     4201 attcctcgct cccacgcagg tcgtcggcat aggcgcaata gtcacagtcg tgtctgtcgt
     4261 ggctatcgcg ctaaacctcc ccgactgatg gctcctcatc cacgcaggcc aacgaaggaa
     4321 gaacttctcg cggcggcggg gaagactgtg ccggacctga tcgccccacg gttgcgcgtc
     4381 ctgttctgcg gtatcaatcc ggggttgtac tcgggcgccg tcggctgcca tttcgcgcgg
     4441 ccgggcaacc gcttctggcc ggcgctttat gccgccgggt tcaccgagcg acagctctcg
     4501 cccttcgagg agcgcgagtt gttgaagagc gggtacggga tcaccaacgt ggtcgagcgc
     4561 gcgaccgcga cggccgccga gctgtcggcc catgaatttg tcgagggcgg gcggcgtctg
     4621 gcggccaagg tcgaacgcta ccggccgcgg tgcctggcag tgttaggaat cggggcgtac
     4681 cgcgccgcgt tcgcactgcc gaaagcccga ctcggccggc aggacgagac gattggcgac
     4741 gccgtggtct gggtgctgcc gaatccgagc ggcctgaacg cccactacca gccgaaggat
     4801 ttagcacgcg tattcgggga gttgcgctcg gcagtcgtgg gtgatcgccg atagggcttg
     4861 tcacccgccg gagggcgtga tagagtgcgc gtcgcattct tcccccacaa gaggaagtct
     4921 gcgaaaaggc cgccggcaca gcgagggtgc cggcggcctt ttcttcttcc ggccaccgaa
     4981 cggtcaggag ggaacacgga tcagacggat gaaaacggat tcgtgtcaat ccgttttcat
     5041 ccgtctgatc cgtgttcccc ggtcgcgcgg cgacgctcaa ccatacccac aatcgaaatc
     5101 cgctctagac cggggaggtt tcgtggatga ttctgcggac gcggtcggcg aacagtacgt
     5161 ctatcttctg cggctcgatg acggccgtca cctcgcccgc gccgaacgac gggtgcgtga
     5221 tgagctgtcc cttacgatat gagcgcgtcc ggtcgtaaag cgcgccgggt tccggggcgt
     5281 cgcccgtgcc cacgagcttc acgccgctct tgaacgtgct gcgcgtgccg cacttcgggc
     5341 aatcgacgcg cgagatgcga ccctgcttcg tgacggacgc caccacgtgc ccccgctcca
     5401 cgccgcagat ggcgcacaac ttctccaccc gattgccgac cacgtatgcc tgcttttcca
     5461 tcaagtcccc ccgacaccca gactgctgga gtttatagac aaaaagtaaa agcccccggc
     5521 gcgtttaccc acgccggagg ctttctcgaa aaagagtcgc cggtctatgc aggcgacttg
     5581 cttagtagcg gttgccgccg ccgccgccgc cgtagccgcc acgtccgccg ccgccgccat
     5641 tgcgaccgcc gccgccaccg ccgcgatcct cgcggggctt ggcctcgtta acgttcagcg
     5701 cgcgcccgcc caggtcgtaa ccgttgaact tcgcgatggc cgcggcgccc tcttccttcg
     5761 aggacatctc gataaagccg aagccgcgcg agcggcccgt gtcgcgatct tcgacgaccg
     5821 cggccgactc gaccgtaccg gcctgcgcga acaactgctg caagtcttcg ctcgaagtct
     5881 ggaacgagag gttgccgacg taaagtttca ttgacataac tgtgtacctc tcccctgtgg
     5941 gagtatcaaa agaagcttcg aattaaaggg gcttcgtctg aacggtagcg gcgaaggctt
     6001 gtgttcgggt gctctcgaag gagcggcttc tgaagtcgaa atcaccctgc tctcggacta
     6061 tccaaacgcc gccgcgcgat tcgcgactgc tgggcgcatc accgttgatg tttgagagaa
     6121 ggaattaacc tgccaagtag gatgccctag ttcgcctcag gttgcaagcg cgcgcaccta
     6181 tatctcactc cccctaacct gtttcttttc tgtcacatca cgacggacga atgcttacac
     6241 aactattaca aacagatggg gcgcgtcttg ctacgatgtc cacgtcgagc gcgtccaatt
     6301 ggacggccgg cgaattctaa ctaacaagag ggcgtgaggg cgtatggaga gactgacaga
     6361 aatcaaacgc gagcgagaga gcatcaactg gacgaccacc attttcgtgt ccatcttcca
     6421 tctgggcgca atcgccgccc tcttcatgtt cagttgggag gcgctggccg tgtccgtctt
     6481 cctgctgtgg ctcgcgggca gccccggcat cggcatgggc ttccaccggc tgctcacgca
     6541 ccgggggttc aagacgccga agtgggtcga gtatctgctc tccatctgcg gcacgctcac
     6601 cctcgaaggc ggtcacatcg actgggtcgt cacccatcgc atccaccacg cgcacacgga
     6661 ggtgccgggc aaagacccgc acacgccgcg cgagggcggc tggtggtcgc acatgggctg
     6721 gattctccgc ggcacggcgc agcagcacgg cgaggcggtg atgatgcgct acgcgcccga
     6781 cctgatgcgg gatcgcttcc acgtcctgct caacaaggtc tactggcttc ccctcgtcgt
     6841 cgtcggcgcg gcgctcctct acttcggcgg ctggtcggtc ttcatgtggg gcgtcttcgg
     6901 gcgcgtggtc ttcgggtggc actccacctg gttcgtcaac tcggcgacgc acctgtgggg
     6961 caagcgccgc ttcgtcacca cggacgactc gaccaacagt tggtgggtgg cgctcctcac
     7021 cttcggcgag ggctggcaca acaaccacca cgcgcacccg cgcgccgccc gccacggact
     7081 catgtggtac gaggtggaca tgaactggta cggcatccgc gcgctcgaac tgatgggcct
     7141 tgccacagac atcaagctca tcagcccggc cgccatccgg gccgcagccg cagccgcagc
     7201 cgagccggag ttactcaggc gcgcggcata gcaaggcaaa agtaaaaagg caaaaggcaa
     7261 aagtgaaaga gcaggccgcc tcttcacttt tgccttttgc ctttttactt ttgccttctc
     7321 acttttgcct tcctacaatg ccgccatgcg tatcggcggt cgtcgcaaga cggtcatctt
     7381 ctttctgacg ttcggaattc tcctggtcgc gctggcgctg gcgctcaacg tcgggtggat
     7441 tctgctcaac tggcgtcagg tcgcgctcct cgtcctcggc atcgtcttct tcaccctcat
     7501 catcaccggc ctcgtcctca acacgacttt cctcatccgc gaaatccgcc gcaacgagca
     7561 gcacgacgcc ttcatcaatt ccgtgacgca tgagctgaag acgccgattg cctcgatccg
     7621 cctctacctc gaaaccctga agacccgcga cgtgaacgag gcgcagcgca aagagttcta
     7681 cgacatcatg ctcgccgaca gcgaccggct cctgcaaacc gtcgaacagg tgttgcgcgc
     7741 cgggcgcgcc tcgcacaagc agcggctcgc caacgtctcg gtcatcgacc tcggccgggt
     7801 cgtgcgcgag tgcctcgacc tgacgcgcgt ccgctacaac ctgaaccccg acgcgctccg
     7861 ttacgtcgag tcgatgggcg acgcgcgcgc ccgcgtcgcg ggcgatgaag acgagctgcg
     7921 cgccgccgtt tcgaatcttt tggagaacgc cgtcaagtac tcggacaagg aggtgcatat
     7981 ctccgtcgag gtggcggcgc tcgacgagcg gcgcttggcc gtgcgcgtcg ccgaccgcgg
     8041 cgtcggcatt ccgagcagcc agctcaagcg catcttcaaa cgcttctacc gcgtgcaggg
     8101 cggcttcatg aagcggttca aagggacggg cctcggactc ttcatcgtgc gctccatcgt
     8161 cgagaagcac ggcgggcgcg tcttcgccga gagcgcgggt ctcgggcagg gcagcacgtt
     8221 caccatccac ctcccgaggg cataaaaagc gatgagcgcg gtgctaatcg tcgaagacga
     8281 acaacatctg gccgacggcc tgcgtttcaa cttggaggcc gaaggctaca cggtcgagac
     8341 ggtggccgac ggcgaagccg cgctcaccct cctcatagac cggcgcgagc acttcgacgc
     8401 cgtcgtgctc gacgtgatgc tccccggtaa ggacggcttc accgtcgcgt cggaactgcg
     8461 ccaagccggg cgcttcgtgc cggtgctgat gctcacggcg cgcggccgcc ccgaagacgt
     8521 gctgcgcggg ttcgagtcgg gcgcggacga ctacctcccc aagccgttcg agctggcaat
     8581 cttaatcgcg cgtctcaacg gactgctccg ccgccgcggg tggttccaac tcgacccgga
     8641 catctcctcg ccaccggacg ggccagccgc cccgtcgggc gcgtcccagt ctgaaagttt
     8701 gaaggacggc aaacgggcgg ccgatctcgt gggcgagctg ttcgtcttcg acgggaagac
     8761 gattgacttc ggggcgctgg aactgcgaac ccgagaccag accatgcgtc tgaccttgat
     8821 ggaggcgcgc ctcctccgtc atctgatcgg gcacgcgggg cgggtcgtgt cgcgcaagtc
     8881 gctgctcgaa gacgtgtggg gactacagga agacacggac acgcgcgcca tcgacaactt
     8941 catcgtccgg ctcagaaaat acatcgaaga cgaacccgcc cggccccggc acctcctgac
     9001 ggtcaggggc gtcggctatc gcttcgtcgc cgagccgggc cgcccagccg cctgaacgcg
     9061 tgtcgccaca ctcaaccgaa aactgttaga ggagggaatg cgtgatgttc aaatcgaagt
     9121 cggcgtcctt cgtcgtcgct gcgctgttgt cggtcgtcgg ggcgcgcgcg gcctgcgcgc
     9181 aggccctgta cgagctgccg gagaatgtgg agacgcgttg ggcgagcccc gagaacccga
     9241 ggggcgagaa ggggaggggc gcgcaggcgg cgggcgggcg caagggttcg ccgacgattg
     9301 ccatcagggc gggcgcgagc gccgtgctgg ccgaggcgca gaacacttcc ggcatggtgc
     9361 gacgcatctg gatgaccatc cctgagcgct cgccgcagat gctgcggggc ctgcgtctcg
     9421 acatgttctg ggacggcgcc gcgcgagcgg ccgtgagcgc gccgctcggc gatttcttcg
     9481 gcgtcggcct cggccagatg gcgcagttcg agtcggcgct cttctcgaat cccgagggac
     9541 gcagcttcaa ctgcgtcgtg ccgatgccct tcaagaccgg catgaagatc gtgatgacga
     9601 acgagagcgg cagagacctg ccggaactct tctacgacgt ggactacacg ctcggcgacc
     9661 gccaccccgt cggcacgctc tacttccacg ctcattggcg gcgcgagaac ccgacgacgc
     9721 tgcaacgcga ctacgaaatc ctgcccagcg ttgcgggtcg cggccgttac ctcggcgcca
     9781 acatcggcgt catcgttgac cgcgacacct acttcaacac gtggtggggc gagggcgaag
     9841 tgaaaatcta cctcgacggc gacgcgcagt ttcccacgct cgccggcacg ggcgcggagg
     9901 actacatcgg cacggcctgg gggcaggggc gcttcgcgca cctctatcaa ggctcgcccg
     9961 tcgccgacga gcagaacatg cgctggtgct tctaccgcta ccacgtcgtc gatcccgtct
    10021 acttccgccg cgacgtgcgc gtcacgatgc agcagatcgg ctacctcgcc gaccacagcc
    10081 gcggcggact ggcgcgcacc gcacgccgcc tctaccgcgc cggccccggc ctcgtcgaga
    10141 aggacatcaa agaggacggc aagttcgagc gcacggacga ctggtcgagc tgcgcctact
    10201 tctacctcga ccgccccgag aacaatctgc cgcaactcga cgcggcggaa aagagaatgg
    10261 ccgggttgtg agacttaatc ggccgtgaga ttcaaacgcg tgaggaagtt ggggcgcgcg
    10321 gcgtcggctt ccgttttcat cctcctgcta tggtcgtgtg cggcgcagac cgcggcgcag
    10381 gagccgaact acgcgcgtct ggagcgggcc gcggagatga tccggcgggg gcaactcgcg
    10441 caggcggaga cggagttgaa caccgtccta cgcgcgcgtc ccgaggaggc gaacgcgctc
    10501 aacctgctcg gcgtcgtgcg cgcgcagcag aagcgtttcg aagaggccga gcggttgttc
    10561 ctgcgcgccg tcaagagttc gcccacactc gtcggagcct tcgtcaacct cggccagctc
    10621 tacatcagca ctggcgacga agagcgcgca ctgcgggcct tcgacgcggc ggccgcgctc
    10681 gcgcccgacc accccgaggt taactatcac ctcgcggcaa tccgcgaagg gcgtcgggag
    10741 tttgcgcgcg cgctcgaaca cctcggaaag atacccggcg agttgcgcgg ccttgagcat
    10801 ctgcaactcg tcgtcaggtg tctgctcgga ctcggccgcg cggacgaggc ggcgggcctc
    10861 gtcgccccgc tcaggtcagc tcagaacacc gccgccaccg tcccgcccga agacgcggcc
    10921 ggcttcgccg ccctcttcgc cgctcacgcc cgtactgacg tggcgctgga aatcttgggg
    10981 gccgcgcgcg ggcgcgcgcc cgactccttc gccgtcctct acaacctcgg cgcgacacac
    11041 ctccagcggc gcgagcttga gcgcgccgaa gagttctacc gccgcgcgct cgccgcgcgt
    11101 cccgacgacg gcgcgacgct ccgcgccctg gcgggcgtag aacgcgcgcg cggcaatctc
    11161 gaaaaatctc tcggctacgt cgagcgcgct ctgcgactcg accctgactc ccgcgcgctc
    11221 ctctacgact acggctggac ggccctcaac ctcgaccgca tcccccaggc cctgcccgcg
    11281 ctcgaacgcc tccaccgcgc acacccgcag gagtccgcgt acatctacgc gctggccgtc
    11341 gcccgcttct acaacaacga ggacgcgcag gccctcgccc tcctcagacg ctacatcggt
    11401 ctcaacccgc gcgacccgcg cggccactac gtgctcggcg tgatgctcca actgatgatg
    11461 aggcgcgccg acgcgcgcgt cgcgctcgcc gagagcttcg acctcacccc cacggccgac
    11521 gcagcccact acctcggcct cgtcgcctac gacgaggccg acctcgcgca ggccgaacac
    11581 tggctccggc gcgccgtcga actcgaaccc tcgcacgcgc cggcccgcgc cgccctcggc
    11641 atgacctacg cccggcgcaa agacctcgcc gcggcgcgcg ccgagcttga gcgcgccgcc
    11701 gcgctcgacc cgcaagacct caccgcggct taccaactcg gcctgctcta cacgcgcctc
    11761 ggcgaacggg agcgggcgcg cgccatgttg gccgtggccg accgcctgcg cgccgagcag
    11821 cgtcagaccg ggagggccgg attgaggctg gccgagccgc cgaagtgagt cgttcgagca
    11881 agtgagtttg cgcgcatccg agtcgtccgc gcgagcgcgc aaaaccccgg tcgtagagtt
    11941 cgctcccgta cagaggccgc ccggccgccg aaatttactg ttgacaaggc gagagcgtcg
    12001 ctgttataca cctgcccatc cggttaaccg tttgcgtaaa cgatttccgg cgacaatcct
    12061 ggattcttac ttggagttga aagtccccgc gagacgcccg gaggcggtcg tggggcgcac
    12121 cgccttccgc cgccgcgtcg gggaagggat gccaggtttc ctctcgcggt aaagcccttt
    12181 ggggcgcgcg cgcgtcttcg gcgtttccca tctcggcgca ggcaaagcaa caggagggtt
    12241 catgaaagtt aacaggctcg tcgtgctcgc actgctggcc ctctgctcga cgtggggcgt
    12301 gagcgcccac gcccagagcg accgcggaac catcaccggc accgtcacgg atcagagcgg
    12361 cgccgtcgtc gccgcggcga aggtcaccgc cacggccacc gacaccggcg aggtgcgcga
    12421 gacgaccacc tccggcgaag gctcctacac cttcgccgag gtgaaggccg cgccctacag
    12481 aatctccgtc gaggctcagg gcttcaagac ggcgaccacc gaggacgtga aggtcgccgt
    12541 gcaggtgacg cgcacggtgg acatcacgct ggaagtcggc gtcgtcggcg acacggtcac
    12601 ggtcacggcc gaggacacgc ccgtcattca gacggagagc gccgtcaggc agacgaacgt
    12661 caacgagcgg caggtgaagg agctgcccct cgccgtggcc gccgagtcgg gcgggcgaac
    12721 ccccctggcc ttcatcttcc tcgacagcaa cgtgagcgcc ggctccggca ccacggggcg
    12781 gggcaccgac gcctctaact tccgcgtcag cggcgggcag ggcctcggca ccgagattct
    12841 gatcgacggc gccagcacgc ggcgcgcgca gaacggcacc ttcttctccg aggtcgcgcc
    12901 ggggccaaac gccttccagg agttcaccat ctcgacttcg agctactcgg ccgagttcgg
    12961 cagctcctcg ggcggcatcg tcaacttcac cctcaagtcc ggcggcaacg actttcacgg
    13021 cgagctctac gagctgcacc gcaacgacgc cttcaacgcc aacacctacg tcaacaacgc
    13081 cctcgggctg gcgcgtccgc gcgaccacca gaacaacttc ggcggcaaca tcggcggccc
    13141 catctacctg ccgcgcttcg gcgaaggcgg cccggcctac tggagcggca agaaccgcgc
    13201 cttcttcttc ttcaactatg agggcttccg cttcgcccga caggagaaca cgctggtgag
    13261 cgtgcccacg gcccgcatgc gcgccggcga cttctccgag cttctgaccg acccctacgt
    13321 gctgtcctcc ttaggcggcc ccgtccaaat ctacgacccg acccaggcgc ccggctcgcg
    13381 caccgccatc cccggcaacc gcctcgacct ctaccgcggc ggcgccgtgc tcgacccggc
    13441 cggggtagcc ctcgccaatc tcttcccggc cccgacgcgc gccggcgtct tccgcaacta
    13501 cgacgcgcgc agcctctcgc cgacgacgat ggacaactac gtcggcaaga cggacttcat
    13561 gctctccgac cgccagcgcc tcggcgtcag ctactcctac cgcaagctcg acagcatcaa
    13621 gggcttcggc gggcgcccgg ccttcacgcg cctgccgccg cccttcacgc agcaggaggt
    13681 ctggaaccag gccttcaagt cgcacttcgc gcgcgtgcag cacgacatca ctctttcgcc
    13741 cacggtcatc aaccacctca acctcgggtt cacgcgctac gacgtggcga acatgaactt
    13801 cggcgagggc atccagccca gctccttcgg cgtcccggcc ggagcgacgg ccaacttcgc
    13861 gctgccgcgc gtcggcttcc ccggctacgg cgacttcatc acgggcggcg acccgcgctc
    13921 ctacatcgac gccggctcga ccttcttcac cgaccacctc tacgacaaca cggtgcagat
    13981 ctccgacgcg ctgacgtgga tcaaggggcg gcacacgatg aagttcggcg gcgacgtgcg
    14041 catccagcag ttcaacgtca cgcagcagat cgacccgggc ggctccttca acttccgtca
    14101 cgaccagacg gccgagaacg tcgtggtggg gactaacgag gagggcaacc ccatcaccca
    14161 ggtgcagggc ggctggccca tcgcgagcct cgtcacgggc gccaccgagt tctccttcca
    14221 gaccgtgacg gccgtcgatc ccggctggcg ctacttctac ccggccgtct tcttcaccga
    14281 cgacatcaag ctcacgcagc gcctgacctt aaacgtcggc gcgcgttacg aaatctccta
    14341 cccgcgcacg gagtcgaagg gccgcctgcg cggcttcgac cccgacgcca tcaacccggc
    14401 cgtcggcatc cgcggcgcgc tcgtcggcgc ggccggtcag ggcggcgtct cggccgagca
    14461 cgagggactg gcgcgtcccg actactcgaa cttcggcccg cgcgtcggct tcgcctatgc
    14521 cctcaacgat aagaccgtgg cccgcggcgg catcggcatc tactacgcgc cgatcctcta
    14581 cggcttcgag ggcggcaaca ccatcgaggc gtcgttaggc taccgcaact ccgccacgcc
    14641 caacgacggc tgcccgcgct gccgccgccc cgacttcggc tcgctccagg cgcgctactt
    14701 cctgcgtaac ctgcccgcct cggggccgct cgcgcccacg gccgatccca acgatcagtt
    14761 cgtcaatcag ggcatcgcgg ttgactactt caacaaggac taccgcacgg gccgcaccgt
    14821 ccagtacagc ctcgacgtgc aacgctcgct gccgtcgaag ttcgccgcct ccatcggcta
    14881 catcggccac cgcgccacgc gcctgcgctc gaacttcgag cgcctcaacg ccgtcccgct
    14941 cgacgccctc aagctcggct acccgatcct caatacctcg ctcgccgacg cgcgcaacaa
    15001 cgcggccttc gtcacctacg cgcagtcggt cagcgtgccg ctgccggccg tgccttacgc
    15061 cggctttaac ggcacggtcg cgcaggccct gcggccgttc ccgcagtacg gccgcatccg
    15121 cagccagctt gagagcaacg gccagagctg gtacaacgcc atgcaggcca agctcgaccg
    15181 gcgctactcc aacggcatcc agttcggcgc ctcctacacc ttctcgaagc tcattactga
    15241 cgcctcggaa gacctcttcg gcggctcgcc cctcggcgaa atcctgcaaa acccctacga
    15301 ccggcggcaa ctacgctcgg tctcggctaa caacccgtac cacgtctttg tcttcaacta
    15361 catcctggag ctgcccttcg gcaagggccg ccgcttcgcc aaccgcggcg gcgccgtcga
    15421 taagattctg ggcggctggc aggtgagcgg catccaccgc taccagtcgg gactgccgct
    15481 cgtggtgcgc gacacggagc cgggccgcgc cggcttcctc agccaggtcg gcttcgaggg
    15541 cgcgctccgg cccaacctgt cggggcaacc catcctgacc gggaatcccg agaccggcat
    15601 ccgcttccag ctcgtcaacc ccggagcgtt ctctgcgccg cccaacttcc aggcgccgcc
    15661 aaccgacaac gtgaccaccg cggcctatcg caactactac gccaacccgc tcgccttctt
    15721 cggcgccgcg ccgcccgtca tcgacagcgc gcgctacctg cccttcttca gcgagaacgt
    15781 gagcctcctg aagaagacgc acatcaccga gacgacgacg ctggagttcc gcgccgaggt
    15841 ctttaacgtc ttcaaccggc atcgatactt cggacccgca aacgacctcc ggttcgggga
    15901 cttcggcaac tcgggggcca tcgacaaccg cgacgtatac gcgccgcgcg agattcaact
    15961 cggactccgg ttcatcttct gacacgcccc tcgcgggctt tcggccgtgt gcggcgcggg
    16021 gaggtgactc ggggtcgcct tctcccgcgc cgcacacgct taagggaatg gctcgtctgc
    16081 gcgcgcacag cacccacgcc ctcatctgcg ccgcggccct ctgcgtctcg gccccacacg
    16141 ccgccgcgac caccggcgcc gccgcgcggg cgcgcacccc ggcgcagtct ccgcaacgca
    16201 aaggtggccg ctctccgcgg aagagcgcca acgaagccgc gcgtctggtc ggcgagggcg
    16261 tggccgcgct tgagagggat gagcgagcgc gcgcgcgaga actcttcgag cgcgcgctcc
    16321 aaatcgagcc gagcaactcg gacgcgcaca cctacctcgg cgcgctcgcc gaccagtcgg
    16381 gcgacctcgc cgcggccgag cgacacttcg ccgccgcggc gcgcgctgcc cccgcgtcgg
    16441 ccccggcgcg caacaaccac ggagccgccc tgatgcggct cggccgcacg cgggaggcgg
    16501 cccgcgagtt cgctgcggcg ctccgcttag acgccggcca cgcgggcgcg ctcgtcaacc
    16561 tcgcgcagat acgcttcgcc gccggcacgc ccgacggcct gcgcgccgcc tacgaactgc
    16621 tagagcgcgc ccgcgccgcc gcgcccggcg acgcggagat cgcccgctcg ctcctggtta
    16681 ccgccctgcg cctcggcgag cgcgccgctg tcgcccgcca cttccgcgac tacacgtcgg
    16741 cgctcgcgcg cgcgtcggcg ggcggcgggg acgcggcagc agtcggcacg ggcgcaagcg
    16801 ccttgccgtc ggcgcgcgcg gagttgggag cagcgctttt ggagggcggc ttcgccgaag
    16861 aggcggcgga ggagttggcc gcgggggcgg ccggcgagcc gtcgaaggcc gagtacatcg
    16921 tcatgctggc gcgctcacac atggcgcgca aggacgtgaa ggcggcgggg cgcgcgctcg
    16981 aaggcgcgtt ggcgcggggg ctggaggccg cgccgatcta cgcggcgctg gccgaggtct
    17041 acgaggcgag cgggcacgtc gagaacgcca tcccggcgat gcgcctcgcc atcgagcgcg
    17101 acccgcgtaa cgagtcttat cgcttccgct acggaatgct cctgacggac acgcacgcgc
    17161 cggcggccgc cgtcgtccgg ctggaggagg cgcttaagga gttcccgcgc tcttcgcgtc
    17221 tgtggttcgc gctcggcgtc gcgcaagccg cgctcaaccg cgcggacgag gccgccaaat
    17281 ccttcgagcg cgcccgcgac ctcgacccga agttcgcccc ggccctcgcc tatctcggca
    17341 tgacgcacga cgtgcgggga cgcttcgcgg aggccgtcga actctacgag cgcgcgctcg
    17401 ccctcgacga ccgcctggcc gccgcccact acctcgccgc cgaggcgata ctcaagcagg
    17461 cgccggccga cgagacgcgc gccgagccgc acctcaaacg cgccgtcgaa ctcgatcccc
    17521 gattcgcgcc cgcgcgcgtg tcgctcgcga aaatctacat gcgcggcgaa cgcttcgagg
    17581 agaccgtcgc gcagctacag tcggccgtcg ccgccgaccc cgcgttggcc gccgcgcact
    17641 accagctcgg gcgcgccctt ctgcggctca agcgcaactc ggaggggcag gccgcgctcg
    17701 ccgagttcaa gcgtctgagc gagggtcagc gcgagcaggc gcgcgaaggg ccgcgcgaga
    17761 tcatgcgccg cctcgcaaac gtccgattct aaaattccgc gggaggtctc atgtcgaggc
    17821 attcggtacg aacgtgcgcg ctgctcttcg cctgcgtctt cctcgggcgg acgaccgtct
    17881 cggcgcaaca ggcgtcggcc acaccctcgc cgtcgccaca ttcgcagcca caacagccgc
    17941 cgccgccgcg cgccttcgac gagtgggccg acggcttcga cggcgacaag ctcgacgagt
    18001 cgaagtggga acgcttcacc ttcgagggag gcggaggggg caagctcgaa gttaaggaag
    18061 gcgagttgcg cctacgaagc ggcggcggca cgcgcgcggg cgtgcggagc agggaagagt
    18121 ttgccggcga gcagttcagc gtcgaggcgc gggtggcgaa ggttggcccg gcgtaccccg
    18181 acccgaacca gaagggtgag ccgatagggt tcgccgcgct gaccatcctc ttcgacggct
    18241 cggggcgcaa ccgcgtcgag tgggtgctga cgagcgccgg gaggttcgag gcgtgggccg
    18301 tggtggacgg ccgcgccgag cagctcgacg accgcaatct cggcacgaag atgaagagcc
    18361 cggtcatcgc catcatccgc cgcggcgaag aattcctctt cgtcttaaac ggccccgaca
    18421 gcccgccgca ggacgcgcgg gtcgggctga ggaagacggt caaaaatttg ccgcgccgct
    18481 tccgcgtcat gctctacggc ttcagctcgt cggagaacaa ctgggacgcg gtgcgcgtcg
    18541 tgacgccacg gcggcaatga accccggccc cctcactcaa ttgagagcac gaagactggt
    18601 gaaaccattc ggcccacgac aaagcgcgcg cgccgccggc ctcctcgccg cgctggccct
    18661 gtgcgccgcc gccgccgccg gcccgtccgt ccgcgcacag cgcgcccccc gcgccggcgc
    18721 cgccgccagc agcgccacct acgccaatcc cgtcttcgac gaagacttcc ccgacccaac
    18781 ggtcatccgc gcctccgacg gttggttcta cgcctacgcc acgaccacca acatcggcgg
    18841 ccgggtctac cacatccagg ccgcgcgctc gcgcgacctc gtcagttggg agcgcctgcc
    18901 cgacgtgctg cccgccaagc ccgcgtggtc gagtcagcac gacctgctct gggcgcccga
    18961 cgtgcaccag cgcggccgca ccttctacct ctacttctcg aatcgcctca acgacgacct
    19021 cgccgcgcgc ttcaaacgtg aaaacaatgc cgagtcgagc aaggaggacg tgttctgtct
    19081 cggcgtggcg acggcccgct tacccatcgg ccctttcacc gacagcggcc gcccgctcaa
    19141 gtgcggactc tcgttcgtca acatcgaccc catggccttc gatgacccgc gcacgggccg
    19201 caagtatctc ttctgggggt cgggttttca gcccatcagg gtgcaggagt tggccgccga
    19261 ccgcctgagc ttcaaggccg ggagccgccc cgtcgagttg gtcgcggcca gcaaagacct
    19321 gccgtaccag acgctcatcg agggggcgtg ggtcgtctac cgcggcggct tctactacct
    19381 cttctactcg ggcgagaact gctgccacgg cccgctccag cagatcaagt acgccgtgct
    19441 ggtcgcccgc gcgcgccagg tcgaggggcc ttaccagacg ctcgcgcagg cgcggggcgg
    19501 ccggagcagc gccatccttg agcgcagcga tgcgtggatc gcgcccggcc acaactccat
    19561 cgtcgaggac gacgccgggc agtcgtggat cgtctaccat gccatcgacg tgaagcggcc
    19621 ctacatgacc gccacgctca aggacgaccg caacgtgcgg cgcgtgatgt tgctcgaccg
    19681 cctcgcctac cgcggcggct ggcccttcgt cgaaggcggc ggcaagccct cgcgcggccc
    19741 gaggcccgcg cccttcgtcc gccgcggccg gccgcccccg cgtcggggga gagaatgagc
    19801 ggcgcggcca aaaaagaggc ggaagcccga ccgtcaggga gggcgcctgc tgtcgcacac
    19861 gctacgccct ccctgacggt cgggcttccg ccccatctgc tcaacctgag gcacaaagat
    19921 tgatgaagac acgcgccgtg aggaaaggtt gatgatgacg cgaagcgaaa acatctcagc
    19981 cgcaaagtgg ccgacgctgc tgctcctggc ggcgtcgctg atgctcgcgt gcgcgccgcc
    20041 ggcgcgggcg cgcgtggcgc aggcgtcgtc tgaggcgccg cgcgccgagc acccgcgccc
    20101 cgacttcgtg cgcgccgact ggcagacctt gaacggccgc tgggagttcg agttcgacga
    20161 cgccgaccgc ggcctcgccg agcgctggta cgaccttgcg gcgggcaagg gcttctcgaa
    20221 aacgatcctc gtccccttcg ccttccagac ccgcctgagc ggcgtcggcg tcaccgactt
    20281 ccacgacgtg gtctggtaca ggcgcgccgt gcaggcgccc gactcgttcc gccgccccgg
    20341 ccgccgcatc ctgctcaact tcggcgcggt cgattacgag gcgaccgtct gggtcaacgg
    20401 cgagcaggcg gggtttcacc gcggcggcca cgtctccttt tccctcgacg tgaccgacct
    20461 cctcaaggcg ggcgacaact cggtcgtcct gcgcgtcttc gacccttcgg ccgaccgcac
    20521 cgtcccgcgc ggcaaacagt tctggcggcc caagtccgaa ggcatctggt acacgcgcac
    20581 caccggcatc tggcagcccg tgtggatgga gtcggccggc gacacacacg tccgccgcct
    20641 gcgcgtcacg cccgacgtgg acaactcgcg cgtctccgtc gaggccgtcc tcaacaagac
    20701 ggcgccgggc ctgcggctgc gcaccgtcgt caagctcaag ggcgaggtgc acgcgcagtc
    20761 ggaggtcgcc tgcgcggacg cccgcccgct cgccgtccac aagctgaacg aacagcgtct
    20821 gtggtcgccg tgggagccga ctctctacga cctgacggtg gagcttgtcg gtgcgggcgg
    20881 gcaggtcgtg gaccgcgtcg agagctactt cggccagcgc aaagtgagca cgcacgacgg
    20941 ccgcgtctac ctcaacaaca atccgtacta cctgcgcctc atcctcgatc agggctactg
    21001 gcccgaaagt cttctcaccc cgccgaccga cgaggccatg cgccgcgaca tcgagctgtc
    21061 gaaggcgatg ggcttcaacg gcgcgcgcaa gcaccagaag gcggaagacc cgcgctggct
    21121 ctactgggcc gaccgcctcg gctacttcgt ctggggtgag atggccaacg cgcaggacta
    21181 ctcggaggag tacgccgcgc gcttcgccga cgagtggcag cagatagtgg cgcgcgactt
    21241 caaccacccg tcgatcatcg tctgggtgcc catcaacgag tcgtggggcg tgcccgacat
    21301 cttcacgagc cgcgcgcagc aggatcacgc ccggacgatg tatcacctgt tgcgctcgat
    21361 tgacccgacg cggctcgtca tcgacaacga cggctgggag cacaccgacg agaccgacct
    21421 cttcgccctc cacgactacg cccgcacggg cgaggagctg gccgagaagt ataaaatcct
    21481 ggagaccgac acgagccgca tcccgcgcaa cgggcgcgac gcgctcgccc tcggctacaa
    21541 gtacaacggc tcgcccttcg tcctgacgga gttcggcggc atcgcctacc gcgtcggcgc
    21601 gccgaaggcc gcgaacgagt ggggctactc cggcatcgag ccgacgaagg aggcgatgct
    21661 caagcggctc gacgggctgg tcaaggccgt cgtcgccaac aaggcgtggg cgggcttctg
    21721 ctacacgcag ctcacggacg tggagcagga gatcaacggc ctgctgacct acgaccgccg
    21781 gccgaaggcc gcccccgaag agttcaagcg aatcttcgag cagcggtaga ggcggccgac
    21841 cggcccggcg gttcgactcc cggccccgtc acgggggctg tttgagagaa cgatggaacg
    21901 cgagacaaaa acggcgcgcg tcggaggcgc gagatgatcg acacggggat gctgcgccgc
    21961 gccttcaggc gtctgagcgc ggaagagccg cgcctgttcc gcgcgcccgg ccgcgtcaac
    22021 ctgatcggcg agcacactga ctacaacgac ggcttcgtcc tgccgatggc catcgaccgc
    22081 ggcaccgtgg tggcggctgc tgcgcgcccc gaccggaagg ttcgcgccta ctcgctgaac
    22141 ctcaaccaga cggtggagtt cgacctcgac ggccccggcg caggccgccg cggcgtgtgg
    22201 ctcgactacg tcgagggcgt cgcgcgcgag ctggagaagc gcggcacacg cttgcgcggc
    22261 gccgacctcg cactcgattc cgacgtgccc gtcggcgcgg gcctctcctc ctcggcggcg
    22321 ctcgaaatct cggtcggcct cgccctggcc tcggtcgcgg gcgccgagat cgacggcgtc
    22381 acgctcgcgc tcgccggcca gcaggccgag cacaactacg tcggcacgca gtgcggcatc
    22441 atggatcagt tcgtcgccgc gatggggcgc cgccggacag cgcttctgat agactgtcgc
    22501 acgctggcga cgatccacat cccgctcgac tcggcgagcg ccgccatcgt catctgcgac
    22561 acgcgcgtca agcacacgct cgccaactcc gagtacaaca cgcggcgggc tgagtgcgag
    22621 cgcggcgtgg agattctcaa gggactgctg ccgcaggagg tgcgggcgct gcgcgacgtg
    22681 tcggtcgagg atttcgagcg gcatcaggtg agtctgccgg agcctgtgag gcggcgcgtc
    22741 cgccacgtcg tgacggagaa cgcgcggacg cttctggccg ccgaggccct gcgcgccggc
    22801 gacctcgaag gcgtgggccg gctgatggcc ctctcgcacc gcagcttgcg cgacgactac
    22861 gaggtgagtt gccgcgagct cgacacgctg gtgcgcgtgg ccgagacgac cgagggcgtc
    22921 atcggcgcgc gcatgacggg cgccggcttc ggcggatgca ccgtcaacct cgtccgccac
    22981 ggcgccctcg aaaattttcg caagaccctc tcgctggaat gcaaccgcgc cggccaacac
    23041 gccccggccg tctacgtcgt cgagtcgagc gacggggcga aggaagtgac aactgacaag
    23101 tgataagtga tgagtgataa gtgataagaa agagaaggca gggttttctt atcactcatc
    23161 actcatcact catcacttgt cacgcgaagc ctatgagtag catcagggac gtggcgcgca
    23221 cggccggtgt ttcgaccgcg accgtgtcgc acgtcgtcaa caacacgagg ttcgtctccg
    23281 aggaggtgcg ggcgcgcgtg ctcgcggccg tcgagcagtg cggctactac ccgaacgcgc
    23341 acgcgcgcgg cctcgcctcg ggccggagcc gcatcctcgg cctggtcatc tccgacatct
    23401 cgaacccgtt cttccccgaa ctcgtcaagg ccatcgagac ggccgccttc gagcagggct
    23461 acgacgtgtt gctgtcgaac accaactacg accccgaccg cacgtcgcac tacgtccgcc
    23521 gcctcatcga gcgtaaggtg gccggcgtcg ccctgatgac ctcggaactc gacgccgcgc
    23581 tcatcgacga gctggcccgc cgcgacgtgt gcgtcgtctt cctcgacctc ggcgagccgg
    23641 ggcggcacat gagcaacctg cgcgtggact acgaggccgg catcgaggag gccgtgcgcc
    23701 acctcgtccg gctgggccac cgccgcgtca ccttcgtcag cggccccgcg cacctgcgct
    23761 cggccgagcg ccgcctcgaa gccttccgtc gctcgctcgc gcgccacctg cccggcgcgc
    23821 ccgtcgaagt ctacgacggc gacttccggc tcgacggcgg gcgacgggcc gtgcgccgga
    23881 tgcacgccga cggggagctg cccacggccg tcctcgccgc caacgacatg atggcgctcg
    23941 gcgcgatggc cgagctgcgc gcgctcggcg tcgaggtccc ccgcgacgtg tcggtcgtcg
    24001 gcttcgacaa catcgagttc gccgcgctct cgcaacccgc tttgacgacc gtctgtctgc
    24061 cgcgctccga actcgggcgt cgcgccgtcg aggcgctgct ggaaaccatc gggcacacgg
    24121 agcagcgcgg cgtcgagatc gaaatcccga cccacctcgt cgtccgcggc tcgacggcgc
    24181 gggcgcgcgg cgtggaaaac gattgaacac agcgaaaaag attggttagt gggtcagtcg
    24241 aggaaaaaag aggcggaagc ccgaccgtca gggagggcgc agacggcggc gcacgttgcg
    24301 ccctccctga cggtcgggct tccgcaccgc ctccccaact tgagccacta ccaaagattg
    24361 aacgaggagg agggggacag atgcggaaga cagatttgag tgggcacacg tcctgcgaag
    24421 attcgcgacg ccggtcgcgg cgtgtgcggc gtgcgggccg tgcggcgtgc gcgctcgcgc
    24481 tgttgtttgt tctcagcatc ggcgcggcgg ggcagcggcg cgccgtctac acgaacccgg
    24541 tcgcggccgg agactaccc
//
LOCUS       DASVES010000428        16514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_276214, whole genome shotgun sequence.
ACCESSION   DASVES010000428 DASVES010000000
VERSION     DASVES010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16514
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276214"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2827)
                     /locus_tag="VF240_17500"
     CDS             complement(80..2827)
                     /locus_tag="VF240_17500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177058.1"
                     /translation="MRKLLSLSLCLLVAIFVVAQEEKKSSAGKTSAQGGKSKPAAGKS
                     KPDAVKKTAASRPKPAEAKEDEAALMAELDSLKDLATGERVARLEAFVAAHPQGGGAR
                     VRATELLAGARAALGDERLRAGDRAGGVALFRKVVEGLTPDASDKLFVEVVSRLPANL
                     YATGHRDEGLDLARRVEAKAAGNAKRLLGVAGFYLSVELADDAARAAEEAARLAPESA
                     AARQALGAAHRQALRLERAAEEFARALELEPNSSASRRSLADLRRATGKPDAALLLYR
                     RQLEAEPQDAGARAGLVLSLFEAGRREEGETELLKTLAEQPDNLPLFVGAAYLFASEG
                     DAARALDFAGRAVGLEPRLRWVWARLAYARAMLAQKRPLEAEQAVLLARELGRFPTLD
                     YELATALAASGRYDEAAEELAKTFALKDGQVETNLAGRNLARAADFIELLAPERRASL
                     FQHKPADTADNARMLKALLAFHLAVGSSESATIDEKGAAAAGLEFVAGSDEMRAFRQL
                     YVAGKLLERGAALPSVVERTDEAMSGVDAATGAPAAVVALFADELRERRAQARSMGAA
                     MSLPSVSRDVMSKILRGRIEDLAGWALYKQGNSAEAVVRLKRALSVLPTTTVWWRSAA
                     WHLGAAHEASGNQRDALASYVGVYKLAPDPARRTVIESLYRRINGTTDGLERLLNNPA
                     EVASRQRPSATTAAPASVEPKQTLERPATEPDKVAAETTPTPAATPTPSPEEPAPLPT
                     PTPDATPAAPEPTAMPTPEPMPTPTPEPTPTPTPSAPETPESPAPTPTPTPDATATPM
                     TVAANTTATTTAEATPAKPTTGDGACALTISEEAVSLQTGGGSAVVTVGLGGAGDLSK
                     VSARTVNWADIVILAEPRKPDDDTAAQRYSISSVSGKPGRYTVTFTSPCGKRDVQVEV
                     K"
     gene            complement(2969..4768)
                     /locus_tag="VF240_17505"
     CDS             complement(2969..4768)
                     /locus_tag="VF240_17505"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7177059.1"
                     /translation="MTNTSRLLYPKSARAARSLALAIIFTLLPAAPAASQRRSSPPQT
                     PAADRRVAPMAATTATTGQRPRLVLLIAVDQFRYEYLERFGDLFSQGGLRRLLRGGAS
                     WTQANYDHVPTETAPGHATMMTGTWPSENGIIGNDWFEREPEGDKGRRVNNVEDEKAK
                     LFGGGASERAASPRRLLASTLGDEMRLASAGRSKVVGVSLKDRSAILPAGRHANAAYW
                     YSPETGRMVTTDYYMAQPPAWVARFNQTPPADKYVGAAWERLLTDASVYTRRAGPDDP
                     EWEWKPAGAERDSSVFPHKLAAGAPDRKFYDALQSTPFANEITLDFAKEAIVNEALGA
                     DDDTDVLTVSFSANDYVGHRFGPYSQEAMDMALRVDKQIESLLDFVERRVGLARTLVA
                     FTADHGVAPVPEHAATLNLPGRRVKQDDVRAKVKEAFRARFGRPTDRDRGDDYLLEYT
                     AKNGQLYLNLAALRRDGVSREEAERVAGEAALQLPGVARYFTRTQLTGGGISPADAVA
                     RRVLHGYNARRGGDVVIVMEPYILVVTYTADHYSPYSYDTHVPLIIMGAGLAPGSYRQ
                     PATPADIAPTLAALLRLLPPSSATGRVLEEAIR"
     gene            4888..5424
                     /locus_tag="VF240_17510"
     CDS             4888..5424
                     /locus_tag="VF240_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177060.1"
                     /translation="MEEPERELTEHLLEAARRVGFSAAGGEDTDLSNYLSLRAANDQL
                     RQRGAGALLDAFTALAGEANRRGAAVTLARTEPHRFHVGNSTMVGTRLTLSRGVRSLA
                     VETGWPRAPRDGFVQGGGLARARVTHFGDRSAGEDLMLVLDVGGTPVWVVPGGGSAHA
                     ELTPERLCQHVIRLLSEK"
     gene            5763..6374
                     /locus_tag="VF240_17515"
     CDS             5763..6374
                     /locus_tag="VF240_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177061.1"
                     /translation="MTNRRTNLLAFAAAALFSLLLPTAANAQQGPWWGQGRNDDNYGR
                     RNGRLSDQDRRVLRDVARRIDDRSGSFQRNVDRALDRSRIDDTPREDNINDRVRDFRR
                     AAERFRNRAGDSNNINNSRGEAQQLLNSAANIDRLVRRVRLDGRAQSDWSQIRADLRT
                     VASFYNLRLSDYDSNDGIYGRDDRRDNRRAPTVQWPRNWPRPF"
     gene            6702..7580
                     /gene="rsmI"
                     /locus_tag="VF240_17520"
     CDS             6702..7580
                     /gene="rsmI"
                     /locus_tag="VF240_17520"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX7177062.1"
                     /translation="MDTAAKGTLYLVATPIGNLEDVTRRALRVLTEVSVIACEDTRHT
                     RRLLERFQITTKTVSYHEHNEHERAAALCARLEAGESVALVSDAGTPAVSDPGYRLVR
                     AAIERGLRVVPVPGPTAFVAALIASGLPTDSFYFGGFLPPRAHARRLRFEEVKNLQAT
                     LVFYEAPHRVARALADAREVLGARQAAVARELTKLHEEVVRGALGELCEHFSAEGAAR
                     GEMVLVIDRAATGDAVATQGATAAAPAGLAARVAELEREGLDQRAALKRAARELGLTR
                     DEAYRRLVSERAREKM"
     gene            complement(7677..9056)
                     /locus_tag="VF240_17525"
     CDS             complement(7677..9056)
                     /locus_tag="VF240_17525"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX7177063.1"
                     /translation="MRFYFIEFTYLLAGILFILGLKGLSHPETAKRGMHLAEFGMLMA
                     IVGTLLHFSIVSYELIITGLVIGGLIGGAMAYWVPMTAMPQRTALSHAFGAFAAALVG
                     IAEYMRHGAELGGVKMAAINFEVMLGSLTTTGSLMAFAKLQGMTRGTPMTFRGQNVFN
                     MALLAAVVGLFFYTLVEPGAAAAFYVMIGLAFLFGVMLVLPIGSADMPVVMSLLNSYA
                     GLAAAATGFVLDNKVLIIAGTLDGFSGLILSILMCRAMNRSLTNVLFGAFGSESAAAG
                     AGTGEEGHMREVGPEDVAIQLAYAKQVVFVPGYGMATAQAQHAVRELAGVLEERGVSV
                     KYAIHPVAGRMPGHMNVLLAEANVPYSSLYELEQINPEFPQTDVAVVIGANDVVNPDA
                     RDNPQSPIAGMPILEVDRAHSVIVLKRGKGKGFSGLENPLFFKPVTGMLYGDAKDSLI
                     KLTQAVQQA"
     gene            complement(9072..9416)
                     /locus_tag="VF240_17530"
     CDS             complement(9072..9416)
                     /locus_tag="VF240_17530"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX7177064.1"
                     /translation="MKPEALIAAFYVFALAAFLGYQVISRVPPLLHTPLMSATNAISG
                     ISLVGSIVIAGSEGHDAYRLLGYPLSTLLGFVAVTCATINVVGGFMITDRMLRMFKRK
                     ELPAAKGAKGKK"
     gene            complement(9526..10671)
                     /locus_tag="VF240_17535"
     CDS             complement(9526..10671)
                     /locus_tag="VF240_17535"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX7177065.1"
                     /translation="MNIAVLRETQPGEARVALVPESVKKLIAVKAGVTVESGAGARSG
                     ASDDDYRSAGAEVSDERDAVLSSADVLAAVNRPAEEDMRRLKRDAVLIGFLRPLDEPA
                     SLRPLAELHITSFAMELVPRITRAQAMDALSSMATVAGYKAVLTAADRLPRMFPLLMT
                     AAGTVPPARVLVLGAGVAGLQAIATARRLGAVVDAYDVRAAAGEQVRSLGANFLEVDL
                     GGIETEDKGGYAKELTPEALERGRQLIAKHARTSDVIITTAQVPGRPAPLLVTEDAVA
                     GMRRGSIIVDLAGPSGGNCALSRPGEDTDANGVSIMCPLNLPATVPVHASQLYSRNVT
                     AFLSPLIKDGQLVIDMKDDVVGPSCVTHGGAFVNERVASALGATASR"
     gene            10902..10973
                     /locus_tag="VF240_17540"
     tRNA            10902..10973
                     /locus_tag="VF240_17540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10935..10937,aa:Glu,seq:ctc)
     gene            11370..12299
                     /locus_tag="VF240_17545"
     CDS             11370..12299
                     /locus_tag="VF240_17545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177066.1"
                     /translation="MRRAHILRAAAVVTLVVQFCMAEATAQERGAAQEQPAAVTLTTE
                     AAKAEARAHRARGLEAYRAGRFAEAAREFERATQALPDYAEGYNDLAMARTSLGEFGA
                     VVELLERAVRLQPRRAEMQYNLGTAYYRAGRFEESVAALREAVRLQPAHPWAHGNLGV
                     SLYDLGRYEEAAREFGRAAELQPRRAETHYLLALCHEQAGRRAQAVESLLEAVRLRPR
                     FAEAHNSLGVAYFGDGRHSEATDAFRRAVEIKPDYAEARYNLGAASLAREARDTAVEQ
                     YRALRALDAGLAAKLHNGLFGGMLLDVSGRAPE"
     gene            12338..12967
                     /locus_tag="VF240_17550"
     CDS             12338..12967
                     /locus_tag="VF240_17550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7177067.1"
                     /translation="MDVAGLMTGAGAYTAAGASMDVSGDLIARARRGDEEAFRLIFER
                     WARPVAGFILCMVHGRDAADELAQETFLRAYRNLGRLRDDSKLSTWLFGIARRVALEH
                     LRARRRETSPAEPVGDARRHARAAEGEADSPAALLLDKELGRMVGDALAALDEDKRTV
                     FALKVYQQRSYEEIAEITGFTPAKVRNDLHRARAEMRRRLAPYMGVTAR"
     gene            12964..13860
                     /locus_tag="VF240_17555"
     CDS             12964..13860
                     /locus_tag="VF240_17555"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7177068.1"
                     /translation="MNCEESRPLLEEYFDGELAGAAAARVAAHLASCGSCAALYESLG
                     REQEFYRVAEPEAATAANLWAGVSARLEGERGGGRAARLWRTVRGRAAFAAFGAPRFS
                     PALTAAAVLTAVVLTAGVMTYLNAGRQPQPETASVARQGAAESDIAPRTGNDNERGDS
                     ARPVPARRAEGDAAMKSSASGVGEQRRAVASVVAEGGRRARPHPEVQRRDDTADELVR
                     EAEHKYVAAIALLARDVRRRRSALDSEEAARFERTLAAVDRAIAETRRAARSRPADPV
                     AAQYMLAAYSKKVDLLRDMASH"
     gene            13867..15228
                     /locus_tag="VF240_17560"
     CDS             13867..15228
                     /locus_tag="VF240_17560"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7177069.1"
                     /translation="MRPRAIKTIATLLVAAAAFAPLCVSRVRAAQQQQPPPQQSQTGV
                     VAVPPAGAAGAERASRVSAKLERGGRVAVDNFTTGRIRVTGWDKDTVEATATSERGDE
                     AVTIRIDDTAGGRRVVLDTSYADDDRYKPAGDLNLEKKIGKTVDDRLLQRVVRPDLAD
                     ELSAPSAKARAPRPTVSDKAGAEPRKGEADPTSQADAVKPKPTPAPEVVKPPDDEGAF
                     IRRPREIHIEVRVPRYAELELIRVFRSPVEVSDIDTTVVIVGERGEVRLKGVGAAEVR
                     TRSGRVEVEDVRGVADIFTVGGPIAVRRTPGDVLARTIDGRIELACVGGRVDVTNTDG
                     FTSLAGIGGDVEANTTSGDIAFAGALRAEGRYELRSITGRVEAAIAAAGGAGFNASLS
                     SYRGSVSTDFELKLREDTSRDPVNRRLVGRHGDGRAQLTLDSFEGAVRLAKAPPDAAK
                     LCR"
     gene            15503..16123
                     /locus_tag="VF240_17565"
     CDS             15503..16123
                     /locus_tag="VF240_17565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7177070.1"
                     /translation="MNLSTPPSDAELLRLMSAGDEDAFTNIYRRRQAGVYRFALQMSG
                     SEAVAEDVTQEVFMVLMRDADKFDPTRGSLAAYLYGIARNLVLRSFQKERPLVALSEE
                     DEDEGGAHGELVAHHDPLGDLTRGETVARVRQAVLALPEHYREVVVLCELHEMSYVEA
                     AEALECAVGTVRSRLHRGRALLVERLRHDGRTNAAPQALNTVRCFA"
     gene            16120..>16514
                     /locus_tag="VF240_17570"
     CDS             16120..>16514
                     /locus_tag="VF240_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177071.1"
                     /translation="MNCHNFQSIADELGRGRLMDAAQREDALGHADTCAPCAARLADG
                     RALTAGLRTLAAQSVGQEAPARVESALLAAFRQRHAAAAQLDNAPQTNAPASVVVPLS
                     SHRRGASPWRLPRWAQGAAVAAASVLLVVG"
BASE COUNT         2569 a         5759 c         5640 g         2546 t
ORIGIN      
        1 tctatccacg aagacacacg aagaggcgcg aaccggagtt aactcccatt cgcgcctctt
       61 cgtgtgtctt cgtggatgct tacttgactt cgacctgcac gtcgcgcttg ccgcagggcg
      121 aggtgaaggt gacggtgtag cggccgggct tgccgctgac ggagctgatg ctgtaacgct
      181 gcgcggccgt gtcgtcgtcg ggcttgcgcg gctcggcgag gatgacgatg tcggcccagt
      241 tgactgtgcg ggcgctgacc ttggagaggt cgcccgcgcc cccgaggccg acggtgacga
      301 cggccgagcc gccgcccgtc tggaggctga cggcctcctc gctgatggtg agcgcgcacg
      361 cgccgtcgcc cgtcgtcggt ttggcgggcg tggcctcggc cgtcgtcgtc gccgtggtgt
      421 tcgcggcgac ggtcatgggt gtcgcggtcg cgtcgggcgt aggtgtaggc gtgggcgctg
      481 gagactccgg cgtctcggga gccgagggtg tcggcgttgg cgtcggctcc ggcgtcggcg
      541 tcggcatcgg ctccggtgtc ggcatcgctg tcggctcagg cgcggcggga gtcgcgtccg
      601 gggtgggcgt cggcagcggc gccggctcct cgggcgaagg cgttggggta gccgcgggcg
      661 tgggcgtggt ctcggccgcg accttgtccg gttccgtcgc gggcctttcg agcgtctgct
      721 tcggctcgac gctcgcgggc gcggcagtag tagcggaggg gcgctggcgc gaggcgacct
      781 cggccgggtt gttgagcaga cgttcgaggc cgtcggtcgt gccgttgatg cggcggtaga
      841 gggattcgat gacggtgcgg cgcgccgggt cgggcgcgag cttgtagacg ccgacgtagg
      901 aggcgagcgc gtcgcgctgg ttgccgctcg cctcgtgcgc ggcgccgagg tgccacgcgg
      961 ccgagcgcca ccagactgtt gtcgtcggca gcacgctcag cgcgcgcttg agtcgcacga
     1021 ccgcttccgc cgagttgccc tgcttgtaga gcgcccaccc ggcgaggtct tcgatgcgtc
     1081 cgcgcaggat tttcgacatc acgtcgcgcg agaccgaggg cagactcatc gccgcgccca
     1141 tcgagcgcgc ctgcgcgcgg cgctcgcgca gctcgtcggc gaagagcgcg acgacggccg
     1201 ccggtgcgcc cgtggccgca tccacgccgc tcatcgcctc gtccgtccgc tcgacgaccg
     1261 aggggagcgc cgcgccgcgc tccagcagct tgcccgccac gtagagctgt cggaaggcgc
     1321 gcatctcgtc agagccggca acgaactcca aaccggccgc cgccgctccc ttctcatcaa
     1381 tcgtggcgga ctccgacgac cccacggcga ggtggaaggc gagcagggct ttgagcatcc
     1441 gcgcgttgtc ggccgtgtcg gccggcttgt gctggaagag gctcgcgcgg cgctcgggcg
     1501 cgagtagttc gatgaagtcg gccgcgcgcg cgaggttgcg ccccgccagg ttcgtctcca
     1561 cctgaccgtc cttcaacgca aaagtcttcg cgagttcctc ggccgcctcg tcgtagcgac
     1621 ctgaggcggc gagcgccgtg gccagctcgt agtcgagcgt ggggaagcgg cccagctcgc
     1681 gcgccagcag gacggcctgc tcggcttcga gcgggcgctt ctgcgcgagc atggcgcgcg
     1741 cgtaggcgag ccgcgcccac acccagcgca ggcgcggctc aaggccgacg gcgcggcccg
     1801 cgaaatcgag cgcgcgcgcc gcgtcgcctt cagacgcgaa gaggtaagcc gcgccgacga
     1861 agagcggcag gttgtcaggc tgttcggcga gcgtcttcag aagctcggtc tcgccctcct
     1921 cgcgccggcc cgcctcgaag agcgagagca cgagtccggc gcgcgcgccc gcgtcctgcg
     1981 gctcggcttc gagttgccgg cggtagagca acagcgccgc gtcgggcttc cccgtggcgc
     2041 gacgcaggtc ggcgagactg cggcgcgagg ccgacgagtt tggctcaagc tccagcgcgc
     2101 gggcgaactc ttcggccgcg cgctccagcc gcaacgcctg ccggtgcgcc gcgccgagcg
     2161 cctgacgggc ggccgccgac tcgggcgcga gacgcgcggc ctcctcggcg gcgcgcgccg
     2221 cgtcgtcggc cagctccacg ctcagataga agcccgcgac gccgaggagc cgcttcgcat
     2281 tgcccgcggc cttcgcctcg acgcggcgcg cgagatcaag tccctcgtcg cgatgacccg
     2341 tcgcgtagag gttcgcgggg agtcgcgaga cgacttcgac gaaaagtttg tcggacgcgt
     2401 ccggcgtcaa gccctcgacg accttgcgga acagagccac tccgcccgcg cggtcgcccg
     2461 cgcgcagacg ctcgtcgccg agcgcggcgc gcgcgcccgc cagaagctcc gtggcccgca
     2521 cgcgcgcccc cccgccctgc gggtgcgcgg cgacgaaggc ttcgagccgc gcgacgcgct
     2581 cgcccgtggc gaggtctttg agggagtcga gttcggccat cagcgccgcc tcgtcttcct
     2641 tcgcctccgc cggctttggc cggctcgcgg cggtcttctt cacggcgtcc ggcttcgact
     2701 tgcccgcggc cggcttcgac ttcccgccct gcgcggatgt tttgccggcg gaagacttct
     2761 tctcctcctg cgccacgacg aaaatcgcga cgagaagaca cagcgagagt gaaagtagct
     2821 ttctcataat ttagaactca acaacagtac cgcgagcgga agcctcgcgg gtatgcctgc
     2881 gaccagagtc ccgccgcacg cacgcgagac ccgctcgcta ccgctcgcgg tactgttata
     2941 tctcaacaat cccaacgcgg ttctgtcctc acctgatcgc ctcttcgagc acgcggcccg
     3001 tcgcgctgct cggcggcagg aggcgcagca gcgcggcgag cgtcggcgcg atgtcggcgg
     3061 gcgtggccgg ctgccggtag ctgccgggcg caagtcccgc gcccatgatg atcaggggca
     3121 cgtgcgtgtc gtaggaatag ggcgagtagt ggtcggccgt gtaggtgacg acgaggatgt
     3181 aaggctccat gacgatgacg acatcgccgc ctcttcgcgc gttgtagccg tgcagcacgc
     3241 ggcgggcgac cgcgtcggcc ggcgagatgc cgccgcccgt gagttgcgtg cgcgtgaagt
     3301 agcgcgccac tcccggcaac tggagcgccg cctcgcccgc cacgcgctcg gcctcctcgc
     3361 ggctcacgcc gtcgcgccgc agggccgcga gattgagata gagctggccg ttcttcgccg
     3421 tgtattcgag caggtaatcg tcgccgcggt cgcggtctgt ggggcggccg aagcgcgccc
     3481 gaaacgcctc cttcactttc gcgcgcacgt cgtcctgctt cacgcgccgg cccggcagat
     3541 tcaacgtggc cgcgtgctcg ggcacgggcg cgaccccgtg gtcggccgtg aaagcgacga
     3601 gcgtgcgcgc caagccgacg cggcgctcga cgaagtcgag gagcgattcg atctgtttgt
     3661 ccacgcgcaa ggccatgtcc atcgcctcct gactgtaagg gccgaagcga tggccgacgt
     3721 agtcgttggc ggagaagctg acggtgagca cgtccgtgtc gtcgtcggcg ccgagcgcct
     3781 cgttaactat tgcctccttc gcgaagtcga gggtgatctc gttggcgaag ggcgtgctct
     3841 ggagcgcgtc gtagaatttt cggtcgggcg cgcccgcggc caacttgtgc gggaagacgc
     3901 tcgagtcgcg ctcggcgccc gccggcttcc actcccactc ggggtcgtcc ggcccggcgc
     3961 ggcgcgtgta cacggaggcg tccgttagca ggcgctccca cgcggcgccc acgtacttgt
     4021 cggccggcgg cgtctggttg aagcgggcga cccacgcggg tggttgtgcc atgtaatagt
     4081 cggttgtgac catgcggccg gtctcggggc tgtaccagta agccgcgttg gcgtggcgtc
     4141 cggcggggag gatggcgctg cgatctttga gcgagacgcc gacgaccttc gagcgcccgg
     4201 ctgaggcgag gcgcatctcg tcgccgagcg tggaggcgag cagacggcgc gggctggcgg
     4261 cgcgctcgct cgctccgccg ccgaagagct tcgccttttc gtcctcgacg ttgttcacgc
     4321 gccggccctt gtcgccttcc ggttcgcgct cgaaccagtc gttgccgatg atgccgttct
     4381 cggagggcca cgtgcccgtc atcatcgtcg cgtggccggg cgcggtctcc gtggggacgt
     4441 ggtcgtagtt ggcctgtgtc cacgaagcgc cgccgcgcag cagccggcgc aggccgccct
     4501 gcgagaagag gtcgccgaag cgttcgaggt attcgtagcg gaactgatcg acggcgatga
     4561 gcaggacgag acgtgggcgc tgtccggtcg tcgccgtcgt cgcggccatc ggcgcgacgc
     4621 gcctgtcggc cgccggcgtc tgcggcggtg agcttcggcg ctgactcgcg gcgggggccg
     4681 ccggcagcag cgtgaaaatg atggcgagcg cgagtgagcg agcggcgcgg gcggacttcg
     4741 gatacaggag tcggctggtg ttggtcatgt gtctttggcg aatgggccgc gtgtttgatt
     4801 tagtgggaga cgcggggcat gatagcaccg ccgcgcggac gtgcgcgagc cgcgccggcc
     4861 gacgactttc cccgagagac gaccccattg gaagaacccg aacgtgagct gacggagcac
     4921 ctgctggagg ccgcgcgtcg cgtgggcttc tcggccgcgg gcggcgaaga cacagacctc
     4981 tccaactacc tttcgctgcg cgccgccaac gaccaattgc gccaacgcgg ggccggcgcc
     5041 ctcctcgacg ccttcaccgc gctcgccggc gaggccaacc gccgcggggc cgccgtcacg
     5101 ctggcgcgca cggagccgca ccgcttccac gtcggcaact ctacgatggt cggcacgcgc
     5161 ctcaccttaa gccgcggcgt gcgctcgctc gccgtcgaga ccggctggcc gcgcgccccg
     5221 cgcgacggct tcgtgcaggg cggaggtctg gcccgcgcgc gcgtcacaca cttcggcgac
     5281 agaagcgccg gcgaagacct catgctcgtc ttagatgttg gcggcacgcc cgtctgggtc
     5341 gtgcccggcg gaggcagcgc gcacgccgag ctaacgcccg agcgcctgtg ccaacacgtc
     5401 atccggctcc tcagtgagaa atgatattta agcgcgtcgc ctcgctacga cgggcagacg
     5461 atatgcggcg agccttgcac gatttcgtaa aactcgactg cgtcgattga ttgcctcgaa
     5521 tctctcgatt gattacgtcg cctttgttca aacacgcctg aagtctcgcc tcaacggcct
     5581 tttcccttcc tcaaacactg cggccccgtc ccagctctgt ctgcggcata tcgcttgccc
     5641 tccgcgagcg tggattggtc gcctcttcgc acagtcccca gacggacgcg acccccgacg
     5701 gcgggcttcc caggcaacag cttccgccgc caacacgcac agcaccgaag gagacaacac
     5761 agatgaccaa ccgacgcaca aacctgctgg cgtttgccgc cgccgcactc ttctcgctcc
     5821 tcctgcccac cgccgccaac gcgcagcaag gcccctggtg gggtcagggc cgcaacgacg
     5881 acaactacgg ccgccgcaac ggccggctca gcgatcagga ccgccgcgtc ctgcgcgacg
     5941 tggcgcgccg catcgacgac cgcagcggca gcttccagcg taacgttgac cgcgcgctcg
     6001 accgcagccg catcgacgac accccgcgcg aggacaacat caacgaccgc gtccgcgact
     6061 tccgccgcgc cgccgaacgc ttccgcaacc gcgccggcga cagcaacaac atcaacaaca
     6121 gtcgcggcga ggcccagcag ctcctcaact ccgccgccaa catcgaccgc ttagtccgcc
     6181 gcgtccggct cgacggccgc gcccagtccg actggtcgca gattcgcgcc gatctgcgta
     6241 ccgtcgccag cttctacaac ctgcgcctga gcgattacga ctccaacgac ggcatctacg
     6301 gccgcgacga caggcgcgac aaccgccgcg ctcccaccgt ccagtggccc cgcaactggc
     6361 cgcgcccgtt ctgaaaacag tgagcagaaa gcagtaagga gtcaacgagc cgccggaggc
     6421 ggcggcagag tgtagcccac ggcgcgagcc gtgggaacaa gtcagattaa tgaaccgaag
     6481 ccccggaggg gcgaaagaat cttggccggc gcgaagcgca agctcgcaga ttctttcgcc
     6541 cctccggggc ttcctgttgt ttcctacgac ggtgccacgg gcccagcgcc cgtggctaca
     6601 ttctgccgcc gctcctcggc acgttgactc cttactgctt tcttcctact gcttactgct
     6661 cactgcttac tgctcactgc tcactcccct ataatccccg catggacacg gcggcgaagg
     6721 gcacgctcta tctggtggcg acgcccatcg ggaatctcga agacgtgacg cggcgggcgc
     6781 tgcgcgtgct cacggaagtg agtgtgatcg cctgcgagga cacgcgccac acgcgccgtc
     6841 tgctcgaacg ctttcagatc acgacgaaga ccgtcagcta ccacgagcac aacgagcacg
     6901 agcgcgccgc cgcgctgtgc gcgcggctcg aagccggcga atcggtggcg ctcgtctcgg
     6961 acgccggcac cccggccgtc agcgaccccg gctaccggct cgtgcgcgcc gccatcgagc
     7021 gcggcttgcg cgtcgtgccc gtgccggggc cgaccgcctt cgtcgccgcg ctcatcgcct
     7081 cgggcctgcc gaccgacagt ttttacttcg ggggcttcct ccccccgcga gcgcacgccc
     7141 gccgcctgcg cttcgaggag gtcaaaaatc tacaggccac gctcgtcttc tacgaggcgc
     7201 cgcaccgcgt cgcgcgcgcc ctcgccgacg cgcgcgaagt cctcggcgcg cgacaggcag
     7261 ccgtcgcgcg cgagctgacg aagctgcacg aggaggtggt gcgcggcgcg ctcggcgaac
     7321 tctgtgaaca cttctccgcc gaaggcgcgg cgcgcggcga gatggtgctc gtcatcgacc
     7381 gcgcggccac aggcgacgcg gtcgccactc aaggcgcgac tgccgccgcc ccggcgggac
     7441 tcgccgcgcg cgtcgccgaa ctcgaacgcg aaggtctcga ccagcgcgcc gccctcaagc
     7501 gcgccgcccg cgaactcggt ctcacccgcg acgaagccta ccgccgcctc gtctccgagc
     7561 gcgcgcgcga aaagatgtga acgggtaaag acgtgaacgt tgaaaggcgt atgcggccgg
     7621 cgcgccctgg gtgaggactt ttaaaagacg aatcgcccgg ccggcccata agtcgttcag
     7681 gcttgctgca ccgcctgcgt gagcttgatg agcgagtctt tggcgtcgcc gtagagcatg
     7741 ccggtgacag gcttgaagaa gagggggttt tccagaccgg agaagccctt ccccttaccg
     7801 cgcttgagga cgatgacgga gtgcgcgcgg tcaacttcga gaatcggcat tccggcaatc
     7861 gggctttgcg ggttgtcgcg cgcgtcggga ttgaccacgt cgttggcgcc gatgacgacg
     7921 gccacgtccg tctgcgggaa ctccgggttg atctgctcca gctcgtagag cgaagagtag
     7981 ggcacgttgg cttcggcgag cagcacgttc atgtggccgg gcatccggcc ggcgacgggg
     8041 tggatggcgt acttcacgct cacgccgcgc tcttcgagca cgccggccaa ctcgcggact
     8101 gcgtgctgcg cctgcgccgt ggccatgccg tagccgggga cgaagacgac ctgcttggcg
     8161 taggcgagtt gaatcgccac gtcctctggg ccgacctcgc gcatgtgccc ttcttcgccc
     8221 gtgcccgcgc ccgccgcggc cgactcgctg ccgaaggcgc cgaacaggac gttggtgagc
     8281 gagcggttca tcgcgcggca catgaggatg gagaggatga ggccggagaa gccgtcgagc
     8341 gtgccggcga tgatgagcac cttgttgtcg agcacaaacc cggtggcggc cgccgcgagg
     8401 cccgcgtagg aattcagaag cgacatcacc accggcatgt cggccgagcc gatggggagc
     8461 accagcatca cgccgaacaa aaacgccagc ccgatcatca cgtagaaggc cgcggccgcg
     8521 cccggctcga cgagcgtgta gaagaagagt ccgacgacgg ccgccagcag cgccatgttg
     8581 aagacgttct gcccccgaaa cgtcatcggc gtgccgcgcg tcatcccctg caacttggcg
     8641 aaagccatca ggctccccgt cgtcgtcaaa gagccgagca tcacctcgaa gttaatcgcc
     8701 gccatcttga cgccgccgag ttccgcgccg tggcgcatgt attcggcgat gccgacgagc
     8761 gcggcggcaa acgcgccgaa ggcgtgcgac agggcggtgc gctgcggcat cgccgtcatc
     8821 ggcacccagt aggccatcgc cccgccgatg aggccgccga taacgaggcc cgtgatgatc
     8881 agctcgtagc tgacgatgct gaagtgcagc agcgtgccga cgatggccat cagcatgccg
     8941 aactccgcga ggtgcatccc gcgcttcgcc gtctcggggt ggctcaggcc cttcagcccc
     9001 aggatgaaga ggatgccggc gagcagatag gtgaactcga taaagtagaa tctcaacggc
     9061 cacgcctccc cttacttttt acccttcgcg cccttcgccg ccggcagctc tttgcgcttg
     9121 aacatgcgca gcatgcggtc ggtaatcata aagccgccga cgacgttgat cgtcgcgcag
     9181 gtgacggcca caaaacccag caaggtgctc agggggtagc ctaacagtcg atacgcgtcg
     9241 tgcccctcgg agccggcgat gacgatggag ccgacgagcg agatacccga gatggcgttc
     9301 gtcgccgaca tcagaggcgt gtgcaacagc ggcggcacgc gcgagatcac ctgatagccg
     9361 agaaacgccg ccagcgcgaa cacgtagaac gcggcgatga gtgcttccgg tttcatttca
     9421 cttatgactc ctgttcgttc atcctcacag cgcttcgcct tgcgcctttg cgcctttgcg
     9481 tgaaacttgc tgacgctgtg aagaggtctc tcgcaaaggc gcaatttaac gactcgccgt
     9541 cgcccccaat gcggaggcca cgcgctcgtt gacgaacgcg cccccgtgcg tgacgcagga
     9601 ggggccgacc acgtcgtcct tcatgtcgat gacgagctgt ccgtctttga tgagcgggct
     9661 taagaaagcc gtcacgttgc gcgaatagag ttggctggcg tggacgggga cggtcgccgg
     9721 caagttcagc gggcacataa tcgaaacgcc gttcgcgtcc gtgtcctcgc ccggccgcga
     9781 gagcgcgcag ttgccgcccg aggggccggc cagatcgacg atgatcgagc cgcggcgcat
     9841 ccccgctacc gcgtcctctg tgacgagcag aggcgcggga cggccgggca cctgcgccgt
     9901 cgtgatgatc acgtccgagg tgcgcgcgtg tttggcgatg agctggcgtc cgcgttccag
     9961 cgcctccggc gtcaactcct tcgcgtagcc gcccttgtcc tccgtctcga tgccgccgag
    10021 gtctacttcg aggaagtttg cgccgagcga gcgcacctgt tcgccggccg cggcgcgcac
    10081 gtcgtaagca tcgacgaccg cgccgagcct gcgcgccgtg gcaatcgcct gaagacccgc
    10141 gacgcccgcg cctaggacga gcacgcgcgc cggcggcacc gtgccggcgg ccgtcatcag
    10201 cagcgggaac atgcgcggca gtcggtcggc ggccgtgagt acggccttgt agcccgcaac
    10261 ggtggccatc gaagagagcg catccatcgc ctgcgcgcgc gtgatgcgcg ggacgagttc
    10321 catggcgaac gaagtgatgt gcagctcggc gagcggacgc agcgacgccg gctcgtcgag
    10381 cggacgcagg aagccgatga ggacggcgtc ccgtttcaga cgccgcatgt cctcttcggc
    10441 gggtcggttg acggcagcga gcacgtcggc cgacgagagc acggcgtcgc gctcgtcaga
    10501 gacctcggcg ccggcactcc ggtagtcgtc gtccgacgcg ccggagcgcg cccccgcgcc
    10561 gctctcgacc gtcaccccgg cctttacggc gatgagcttc ttcaccgact cgggcacgag
    10621 cgcgacgcgc gcctcccccg gctgtgtctc ccgcaacacc gcgatgttca tctgaaccct
    10681 ctacctcaac gcttgggctt cgactgagct tcaggcaggc cactgatgtg catgcgccgg
    10741 agtatatcac cggccgcgcg tcgcgctcca aaagttttcg ggcgctaccg cccttcgccg
    10801 cgccgcgcgt ccggccgcaa tgcttgctaa tcactggcgc gctacatata atttcagcct
    10861 gcttcaaagc ggcatcgacc gtggcattgg aagcaggtcg cggcccgttc gtctaggggt
    10921 taggacgtgg ccctctcaag gccgaaacac gagttcaaat ctcgtacggg ctataaagga
    10981 agtgaatcaa tcctcggggc gctcgcatga gcgccttttt gtttttgcct ctcacaccat
    11041 tcccttctcc acactgaatc ccgcccgaaa tttcggattt aaatcgaaga aaacggcaac
    11101 cggcgttcgt caaaaaaagt tgagagcttt ggcgcgcggc cgacactaac tatgcgtgac
    11161 cagccggacg cctttcggca gtagcgcgcg cgtgcgttcg ggaaagtcgg tcaacgccct
    11221 tcggccgggg ggcggtagtt gatgacaggg gacagccgcg gccggcgcta aagtgtcgcc
    11281 gctcggccgc cctttcggta tttaccgccg ccctcgccgg ccacggtctc cgggagttct
    11341 gtagccccgg aagtgaaggg agtttcggta tgagacgcgc gcacattctt cgggcggccg
    11401 ccgtcgtcac gctcgtcgtc caattctgta tggcggaggc gacggcgcaa gagcgcggcg
    11461 cggcgcagga gcagccggcc gccgtgacgt tgaccacaga ggcggcgaaa gccgaggccc
    11521 gggcgcaccg cgcgcgcggt ctggaggctt accgcgcggg ccgctttgcg gaggcggccc
    11581 gtgagttcga gcgggcgaca caagcgttgc ccgactacgc cgagggctac aacgatctgg
    11641 cgatggcccg cacgtcgctc ggcgagttcg gcgcggtcgt cgagctgctg gagcgcgcgg
    11701 ttcgtttgca gccgcgtagg gccgagatgc aatacaacct cggcacggcc tactaccgcg
    11761 cgggtcgttt tgaagagtcg gtcgcggcat tgcgggaggc tgtccgcttg cagcccgctc
    11821 atccgtgggc gcacggcaac ctcggcgtgt cgctctacga cttgggccgc tacgaagagg
    11881 cggcgcggga gttcggacgc gccgctgaac tgcaaccgcg ccgggccgag acgcactacc
    11941 tgctcgcgct atgccacgaa caggcggggc ggcgcgcgca ggccgtcgag tctttgttag
    12001 aagccgtgcg tctgcggccc cgtttcgcgg aggcgcacaa ctcgctcggc gtggcatact
    12061 tcggcgacgg ccgacacagc gaggcaacgg acgccttccg gcgcgccgtc gaaatcaaac
    12121 ccgactacgc cgaggcgcgc tacaatctgg gcgcggcgag tctggcgcgc gaggcgcgcg
    12181 acacggccgt cgagcagtac agggcgctcc gggcgctcga cgcggggctg gccgcgaagc
    12241 tccacaacgg tctcttcggg ggcatgctgc tcgacgtgag cgggcgcgct cccgagtagt
    12301 tcttcaaatt ccgagacggg ggtgggcgca gctgtcgatg gacgttgctg gtctcatgac
    12361 gggagcgggc gcgtacactg cggcgggagc ttcgatggac gtttccggcg acctcatcgc
    12421 gcgggcgcgg cgcggcgacg aggaggcctt ccgcctgatc ttcgagcgct gggcgcgccc
    12481 cgtcgccggc ttcatcctct gcatggttca cgggcgcgac gcggccgacg aactcgcgca
    12541 ggagaccttc ctgcgcgcct accgcaacct cggccgcttg cgcgacgact cgaagctctc
    12601 gacgtggctc ttcggcatcg cgcgccgggt cgcgctcgaa cacctcagag cacgccgccg
    12661 cgagacttcc cccgcggagc ctgtcggcga cgcgcgccgc cacgcacgcg cggctgaagg
    12721 ggaggccgac tcgcccgccg ctctgctgct cgacaaggaa ctggggcgca tggtcggcga
    12781 cgcgctcgcc gcgctcgacg aggacaagcg caccgtcttc gcgctcaagg tctaccagca
    12841 gcgcagctac gaggagatcg ccgagatcac cggcttcacg ccggctaaag tccgcaacga
    12901 tttgcaccgc gcgcgcgccg agatgcgccg ccggctcgcg ccttacatgg gagtcactgc
    12961 gagatgaact gcgaagagag ccgaccgctc ctcgaagagt atttcgacgg cgaactcgcg
    13021 ggcgccgccg ccgcgcgcgt cgccgcgcac ctcgcgtcct gcgggtcgtg cgcggccctc
    13081 tacgagtcgc tcgggcgcga gcaggagttc taccgcgtgg ccgagccgga ggccgcgacg
    13141 gcggcgaacc tctgggcggg cgtgagcgcg cgtcttgaag gcgagcgcgg cggagggcgg
    13201 gcggcgcgtc tgtggcgaac ggttcgcggt cgtgccgcgt tcgccgcttt cggcgcgccg
    13261 cgattcagcc cggcgctcac ggccgccgcc gtgctcacgg ccgtcgttct gacggccggc
    13321 gtgatgacgt acctcaacgc ggggcggcag ccgcaaccgg agacggcgtc cgtcgcgcga
    13381 cagggcgccg cggagtcgga tatcgcgccg cgcacaggta atgacaacga acgcggcgac
    13441 agcgcaaggc ccgtgcccgc ccggcgcgcg gagggcgacg cggccatgaa gtcttccgcg
    13501 tcgggcgtcg gcgaacaacg gcgcgctgtt gcgtcggtgg tcgcggaggg cggcagacgc
    13561 gcgcggccgc accctgaagt ccagcgacgc gacgacacgg ccgacgaact cgtgcgcgag
    13621 gccgagcaca agtacgtcgc cgccatcgcg ctgctcgcgc gcgacgtgcg tcgccggcgt
    13681 tccgcgctcg actcggaaga ggccgcgcgc ttcgagcgca cgctggccgc cgttgaccgc
    13741 gccatcgccg agacgcgtcg cgccgcccgc agccgccccg cagaccccgt cgccgcgcaa
    13801 tacatgctcg ccgcctactc gaagaaagtt gacctgctgc gcgacatggc gagccactga
    13861 ccgacgatgc gcccaagagc aattaaaact atcgccacac tcctcgtcgc cgccgcggcc
    13921 ttcgcgcccc tctgcgtgtc gcgcgtgaga gcggcgcagc agcagcaacc cccgccgcaa
    13981 caatcgcaga cgggagttgt cgccgtgccg cccgccggcg cggcgggcgc ggagcgggcg
    14041 agccgtgtca gcgcgaagct tgagcgcggg ggccgcgtcg ccgtcgataa cttcaccacg
    14101 ggccgcatcc gcgtgacggg ctgggacaaa gacacggtcg aggcgacggc gacgagcgag
    14161 cgcggcgacg aggccgtcac aatccgcatc gacgacacgg ccggcggtcg ccgcgtcgtc
    14221 ctcgacacgt cctacgcgga cgacgacagg tacaagccgg ccggtgactt aaaccttgag
    14281 aagaagatcg gcaagaccgt ggacgaccgt ctcctccagc gcgttgtgcg tcccgacctc
    14341 gccgacgagc tgtcggcccc gagcgccaag gcacgcgcgc cgcgccccac cgtgagcgat
    14401 aaagccgggg ctgaacctcg taagggcgag gccgacccca catctcaggc cgacgccgtg
    14461 aagcctaaac cgacgcctgc ccccgaggtc gtcaagcctc cggacgatga gggcgcgttc
    14521 atcaggcggc cccgcgagat tcacatcgag gtcagggttc cgcgctacgc cgagctggaa
    14581 ctgatcagag tttttcgcag ccccgtcgag gtgtccgaca tagacactac cgtcgtcatc
    14641 gtcggcgagc gcggagaagt tcgtctgaag ggcgtggggg cggccgaggt acgcacacgc
    14701 agcgggcgcg tcgaggtcga ggacgtgcgc ggcgtggccg acatcttcac cgtcggcggc
    14761 cccatcgccg tgcgccgcac gccgggcgac gtgctggcgc gcaccatcga cggccgcatc
    14821 gaactggcct gcgtcggcgg gcgcgtggac gtgaccaaca cggacggctt cacctcgctc
    14881 gccggcatcg gcggcgacgt ggaggccaac acgacgagcg gcgacattgc gtttgcgggc
    14941 gcgctgcgcg ccgagggccg ctacgagctg aggtcgatca cgggccgcgt cgaggcagct
    15001 atcgcggcgg caggcggcgc gggcttcaac gcctcgctct cttcctaccg cggctcagtc
    15061 tccaccgact ttgaactcaa gctgcgcgag gacacctccc gcgaccctgt caaccgccgc
    15121 ctcgtcggcc gccacggcga cggccgcgca caactcacgc tcgactcctt cgagggcgcc
    15181 gtccgcctcg ccaaagcccc gcccgacgcg gcgaagctgt gccgctgaac tcaatgcttg
    15241 aatatgccct gcattgattc accgcggagg gcgcggagat ggcgccgcag ggttgaatcg
    15301 gctcaaccct ctcgcgtgaa ctccgcgccc tccgcgcctc cgcggtgaat ccagtctgcc
    15361 ttacactcaa gtaaaagttg ctctaaactc tcgttggtcg aacacaaagc caatccgccc
    15421 caaacttttt tctggaactt tcccgccccg gcccgcgaat caccagtggc aagcagttgc
    15481 gggccgacga atttaggcgc gaatgaatct ctccacaccc ccaagcgacg cggaactgct
    15541 ccggctcatg tcggccggag acgaggacgc cttcaccaac atctaccggc ggaggcaggc
    15601 cggagtctac cgcttcgcct tgcagatgag cgggtcggag gccgtggccg aggacgtgac
    15661 gcaggaggtc ttcatggtgc tgatgcgcga cgcagacaaa ttcgacccca cgcgcggctc
    15721 cctcgccgcc tacctctacg gcatcgcgcg caacctcgtg ctccgctcct tccagaagga
    15781 gcgcccgctg gtcgcgctct ccgaagagga cgaggacgag ggcggcgcgc acggcgaact
    15841 cgtcgcgcac cacgacccgc tcggcgatct gacgcgcggc gagacggtcg cgcgtgtccg
    15901 tcaggcggtg ctcgcgctgc ccgagcacta ccgggaggtc gtcgtgctct gcgagctgca
    15961 cgagatgagt tacgtcgagg cggccgaggc gctcgaatgc gccgtgggca ccgtccgctc
    16021 ccggctccac cgcgggcgcg cgcttttagt cgagcgcctg cgccacgacg gacgcaccaa
    16081 cgccgccccg caggcactca acaccgtgag gtgcttcgca tgaactgtca caacttccag
    16141 agcatcgcgg acgagttggg ccgcggccgt ctgatggacg ccgcgcaacg cgaagacgcg
    16201 ctcggtcacg ccgacacttg cgcgccttgc gccgcacgtc tcgccgacgg ccgcgcgctc
    16261 acggccggcc tccgcacgct ggcggcgcag tccgtcggac aggaagcccc cgcgcgcgtc
    16321 gagtcagcac tgctcgcagc cttccgccag cgccacgccg cggcggcgca actcgacaac
    16381 gctccgcaaa cgaacgcgcc ggcgtcggtg gtcgtgccgc tctcttccca taggcggggc
    16441 gcttccccct ggcggctccc gcgctgggcg cagggcgcgg ccgtcgcggc cgcctcggtg
    16501 ctcctcgtcg tcgg
//
LOCUS       DASVES010000429        12777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1092338, whole genome shotgun sequence.
ACCESSION   DASVES010000429 DASVES010000000
VERSION     DASVES010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12777
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092338"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF240_17575"
     CDS             complement(<1..443)
                     /locus_tag="VF240_17575"
                     /inference="COORDINATES: protein motif:HMM:NF033579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX7177072.1"
                     /translation="MTNKPKRQYRIRNWHEYNSALVRRGSLTLWVEQRVVGRWHDTAA
                     PVRRGRRRVYSDLAITCALTLREVYQLPLRATQGLVRSVLRLLGADSPAPHYSTLSRR
                     AATLAVKLPRLSQGPLHLAVDSTGVKLYGEGEWKVRLHGTEKRRT"
     gene            811..2004
                     /locus_tag="VF240_17580"
     CDS             811..2004
                     /locus_tag="VF240_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177073.1"
                     /translation="MRKRLFVALAFLTLAGFGFQTASAQLPGGLPRIPRPGKPKATPT
                     PAPAESARPAPKSETQPAAQSQPDTGAAAAPAAGQELPPIAKDWVLVRADTRTSYKGN
                     FDVWSWLPLVTFRSNIERPSGARYYVVVTQPNGAPWIELKCDSDTNGIGHECGSPHTP
                     DEQGINAVGVISFTIKMKNPLAGTDQTLFTGKAKVEKAPSGDHSTRWPNKFSYFVNSD
                     WALPIGYVFKDDYWPAVAFTVRGTNSSGLQLHVFYKGQEVGAQQGPFSCSSTELNGTP
                     TRSTAEAVPQGGTWERVSCSLKGLRWDGEEVEGYHTLVRNPGEYEVKVLWNKKLSRTF
                     KFTVGPDGRLADNGIARANGINAERVIVPVQVVGPQDGQWDRNAWKTEAFFGHPLKGF
                     AGPPQ"
     gene            2071..3273
                     /locus_tag="VF240_17585"
     CDS             2071..3273
                     /locus_tag="VF240_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177074.1"
                     /translation="MRKQLLVFAALLTLACLGPQAVSAQLPGGLPRIPRPGKPKATPT
                     PAPTAQPAPANENRPAAQPQPAAASPAPAQPPAAAPAGGLAVLKHLVRVDTRAVTSYK
                     GDANTYSWTPDITFDTSGGTPSGASYYAVVSLPTGAPWLELECDWAGGGRNKSYFLCG
                     GPKVPEDKGTTATGLFPFAIKLRNELKGTDETLFTGKVKIEKAPGDNGTPAERAKKSF
                     FYPNMDWALPVGYVYYSPADNHLYTGFWVRGSTYKVAPHVFYQGKEIGLVGDCDSNVE
                     VGDAYIRSVKPAPFWKYVECELLSVKIKDERDEFSLMSNPGEYEIKVLWNDELSRSIK
                     FTVGRDGQLAGGPPLLYRVRDLDGERQPGVIVPVAILDAQDGPWDKNAWRTGAFYGNP
                     PTGFSPPQ"
     gene            3473..4720
                     /locus_tag="VF240_17590"
     CDS             3473..4720
                     /locus_tag="VF240_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177075.1"
                     /translation="MGKRIFLALALSSCVGFGLHTAVAQLPGGLPRIPRPGRPKATPT
                     PAPTAQPAPSGETRPAAQPQPDAPAATNPAPPAAAPRPQAGGITLTKTLIRFWPYTVT
                     SYQGNYNNVWSWFPTVRFNTEGGPLPSGGQYYVEVAQPGGGAWVKLKCVNTTIGGPTF
                     ECGDRNDLEQSGTLATGVFPFVIKMRNPLEGTEGKTLFTGRAKVEKVLSNEHGPQAVK
                     KFVYYANQDWNLPIGQVYIDSDDHLNVRFWIRGEGAGGLEAHPMYRGEEVGRYQEHGY
                     QYGGASCSPDIEYFPTQSAGAGAPQEAKWARVNCKFGSVVTKPAKNVPQWHVLGANPG
                     EYEIKVLRNKRLARSIKFSIGPDGKIVDNRIAANSGMGAWDNHILVPVTIIGEQDGTW
                     DKNAWKTEAFYGHPLAGFTWPPQ"
     gene            5221..6525
                     /locus_tag="VF240_17595"
     CDS             5221..6525
                     /locus_tag="VF240_17595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7177076.1"
                     /translation="MKLLLIAPEGLEVQGFKGSKHAHHLNLALIAALATPYFEDIKIV
                     EEEFETLDLDEPADLVGITMMTCQAERGYQLADHFRKRGVRTICGGSHATFMAEECGE
                     HFDAVVVNEVEMIWEEIMADFMADRLKSVYHTDQLLDLSLLPMPRKELFRDNWKGKFA
                     DVIQAGRGCPLGCEFCTVTNMYGKTFRTRPVEHIVEEIKRFKSKRLFFVDDNIFLSRN
                     YAYELCEALIPLKIDWGSQGSMELICRDEELLKLAKRSGCISLFVGIETVDQETLNKN
                     HKQFNKVQKYEENIRKMHRAGINVIGSFIFAFEEDTAECFSKVYDFAVQNRLSMVNCG
                     IMTPFPGSVTYDKARRDKKIVDFRWGQYTGSNLVWRHPNMSRAEVEARYDRFRQQFYS
                     WSSILKRAWRNRAHPLYYWSMNLHLWWLYHVEPRRAPRCLPP"
     gene            6888..8657
                     /locus_tag="VF240_17600"
     CDS             6888..8657
                     /locus_tag="VF240_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX7177077.1"
                     /translation="MEALNHVAHEHERGFHFVGFDSREQFFSYQHVHAEALRRAAHLS
                     ALGLRKGDRLALVVSDGGEFVMSFFGAVMAGVVAVPISSPFTQKAPASYVENVARIVR
                     AAGARMLLTTDKIRPTVQGVSELAGALERLVTVEEAFAGETPAFEPPRVRPEDLCFFQ
                     FTSGSTSSPRGVMISHANLMANVTAFMGPFGIDSRPGDVGVSWLPLFHDMGLIGFILA
                     PLATNRQAVIISTTAFARDPRIWLRTLHKYRGSVTYAPNFAYALTAKRVRDQDLEELD
                     LSCVRVAGCGAEPIHAPTLRSFAERLAPVGFNSDALLPSYGLAESTLAVTLHRAGAPL
                     RTDKIDADALKWGRAAPADEQTKQVSEVVSCGDCFPDHQVVIVDEDGAVLDERRVGEI
                     LARGPSIARGYYNTPEATRATWQDGWLHTGDRGYIADGELFVCGRIKDLIIIRGANFY
                     PHDIESAVRDLPGIRHGNVAAFGVMDGGQEKLTVIAETNGRDDSELGKLIAERVREAV
                     GLEVHRLVFVPSGTLPKTSSGKLQRHKVKELFERGQLTAADAQARRAAPGATGQSQEI
                     SAGGAPPLLGVRQFAGDLEEQAG"
     gene            8706..9596
                     /locus_tag="VF240_17605"
     CDS             8706..9596
                     /locus_tag="VF240_17605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7177078.1"
                     /translation="MSTREDIAVTYDVANDFFRLWLDERMMYSCALFEGTDDLETAQL
                     NKLRYFHDGVRVTPDKRVLDIGCGWGGNLEFLARDMGVRDAVGITLSEAQFEEVRAKA
                     VPGVSVECVSYKDYRPEQKFDAAISIGMFEHVATPEQARSGEQIAVYRDYFRRVWEWT
                     NPGSWFGLQSVISLRIPRDRRDLHRLGWGTSTIFPGAITPRLEAIMASVNPYWEVMEV
                     KTRREHYARTSAEWLRRLKGNEAAIRARWGDELFADYVKYLGGCVMVFDKGYQSLVQI
                     LLRRTDERADFDTLAKMEGK"
     gene            9597..9851
                     /locus_tag="VF240_17610"
     CDS             9597..9851
                     /locus_tag="VF240_17610"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7177079.1"
                     /translation="MTNLDHGDLIKEALAFTAPEEMPQEFSPDSTLGEMGISSITALE
                     MAGYIEEKLSIVFPDDELSQVNTLRDFDQLIRRHTLATQE"
     gene            9871..10743
                     /locus_tag="VF240_17615"
     CDS             9871..10743
                     /locus_tag="VF240_17615"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX7177080.1"
                     /translation="MSVSRAYHEQMYRIYHEFFDVAETKRRWHPFRDLPWEKMDKSKN
                     TEELAICLETFCGVELYVPDYTANAFNMTRTIFGQAWFEANWGYEESKHGLSFREYLV
                     QSGLRTPEQYAQYEEEIFSKVWRLPFTTRRQMTCYGVLQESATYLIYEAQKKRAQQEE
                     NELLEKLFFFISRDEAAHRGFYQKVLVYELEEDRPGTLADLAHVISHFEMPGVPLIPE
                     FQKRLLVNGVGITSQHFLQRGIFPLLKAIGTSRSELIKILGQQKAREAERKKGEEMPP
                     AVPLAEAEPAMASK"
     gene            11026..11853
                     /locus_tag="VF240_17620"
     CDS             11026..11853
                     /locus_tag="VF240_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7177081.1"
                     /translation="MSARWWAGARAAERCVVFIPALAAPQEYCRFFAAFLARQGWGVL
                     TFDYRSVGASKDSASDETATLDDWARLDIPAAVSEARRRSGAQFLAVFAHSIGGQLLG
                     QSPARREIDAALLIAAQRGIPKLFKGRGRLRVSYAYAVFTILIRSLGYLPVSKLTLPE
                     RCSAQALLQWIRWGRTGVFTDASGVNVEPGYAEYRGPLTAVRVADDDYAPAPAVEALT
                     ALYASSPTRRETIEPQKYGPKPVGHFGFFHPRAPRALWSDVESWLRRMEEEARSLKS"
     gene            12118..>12777
                     /locus_tag="VF240_17625"
     CDS             12118..>12777
                     /locus_tag="VF240_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7177082.1"
                     /translation="MQSTPLIFEEIEKAVSLYPEHTALLQLLDGEGEVRYTFRQTLRL
                     AKNLSRALKGRGFGKGDKVVFWAPLSPHWVIAYLGVLYSGCVVVPLDVEYGSDELAFV
                     LAELDSRLIFTTREKLPLLRSATEGRPAEMTVVSLNGVGEADAATAQVEELFEESEAD
                     PAVPPVSPEDVATIFYTSGTTGKPKGVVIEHGAVAAGVLSLKQYIEFIPGDNVLAVVP
                     AH"
BASE COUNT         2467 a         4046 c         4060 g         2204 t
ORIGIN      
        1 cacgtccggc gcttctccgt gccgtggagc ctgaccttcc actcgccctc gccgtagagc
       61 ttcacccccg tcgagtccac cgccaggtgc agtgggcctt gactcaggcg cggcagcttc
      121 actgcgagag tcgccgcccg gcgcgacaaa gtcgagtagt gcggggcggg cgagtccgca
      181 ccgagcaagc gcaacaccga gcgcaccagc ccctgcgtgg ctcgcagcgg cagttggtag
      241 acctccctga gcgtgagcgc gcaggtgatg gcgaggtcgg agtagacgcg acggcggccg
      301 cggcgcacgg gcgcggccgt gtcgtgccac ctgcccacga cgcgctgctc gacccagagg
      361 gtgaggcttc cgcgccgcac gagggctgag ttatattcat gccagttgcg gatgcggtac
      421 tggcgcttag gcttgttggt cacgcatcga gactagcaga cgtaccgcct ccgcgacctt
      481 ttatgcaaca acgcccccgc gccgcccaac aagtcattcg agccgaccgc gactcaacgt
      541 tgctttcatc cgcgagattt gctgctcgtc aatgctcgcc gcggcgctca attccggcat
      601 tagacagttc ttaaggggct agaaagttga acccttgccc ctttccctta ttcggtatag
      661 agtgcgcccc cgcacggggg ttgacctgcc gagttaatcc cccagcgggg cgtggcacgt
      721 ggtgggcctc gctaccggag actttggtgg gcgacgagtt tggagccaca aatggcgctc
      781 acccatttcc caaggcaaag gagaatctag atgagaaagc gtctgttcgt cgccttagca
      841 tttctgaccc tcgcgggttt cggctttcaa acggcttcgg cgcagttgcc gggcggcctc
      901 ccccgcatcc cgaggcccgg caagccgaag gcgaccccga ccccggcccc ggccgagagc
      961 gcgcggcccg cgccgaagag tgagacacag ccggccgcgc agtcccagcc ggacacgggc
     1021 gccgcggcgg ccccggctgc cgggcaggaa ctgccgccca tcgccaaaga ctgggtctta
     1081 gtcagggccg acacgcgcac gtcctacaaa ggcaacttcg acgtctggag ctggctgccg
     1141 ctcgtcacat tccgctccaa catcgagcgg ccgagcggcg cccgctacta cgtcgtggtc
     1201 acccagccga acggcgcccc ctggatagag ctcaaatgcg actcggacac gaatggcata
     1261 ggccacgagt gcgggagccc gcacacgcca gacgagcagg gcattaacgc cgtcggggtc
     1321 atctccttca ccatcaagat gaagaacccg ctggccggca ccgaccagac gctcttcacc
     1381 gggaaggcga aggtcgagaa agccccttcc ggcgatcaca gcacgaggtg gcccaacaag
     1441 ttctcctact tcgtgaattc cgactgggcc ttgcccatcg gctacgtctt caaggacgac
     1501 tactggcccg ctgtcgcgtt cacggtccgc gggacaaatt cgtcgggcct gcaactccac
     1561 gtgttttata aggggcagga ggtgggcgcg cagcagggcc ccttctcctg ctcttccacg
     1621 gagctgaacg ggacgcccac gcgctcgacg gccgaggcgg tgccgcaggg ggggacgtgg
     1681 gaacgcgtgt cgtgtagcct caagggcttg agatgggatg gtgaggaagt tgagggctat
     1741 cacaccctcg tcaggaaccc gggggagtac gaggtcaagg tgctgtggaa taagaagctg
     1801 tcgaggacat tcaagttcac cgtcggcccc gacggcaggc tcgccgataa cggcatcgcc
     1861 cgtgccaacg ggatcaacgc cgagcgcgtc atcgtgcccg tccaggtcgt cggcccacag
     1921 gacggccagt gggatcgcaa cgcctggaag accgaagcct tcttcggtca cccgctcaag
     1981 ggcttcgcgg ggccgccgca gtaggcggcg cactcctaga gacgaggtct catcccccgc
     2041 acgcctattc aagacacaag gagcaccagc atgagaaagc agctactcgt cttcgcagca
     2101 ttattaaccc tggcctgcct cggcccgcag gcggtctcgg cgcagttgcc gggcggcctc
     2161 ccccgcatcc cgaggcccgg caagccgaag gccacgccta ccccggcccc gaccgcgcag
     2221 cccgcgccgg ctaatgaaaa tcggcccgcc gcgcagccgc agccggcggc agccagcccc
     2281 gcgcccgcac agccgccggc cgccgccccg gcaggcggtc tcgccgtcct gaaacatctt
     2341 gtccgggtgg ataccagagc ggtcacgtcc tataaggggg atgccaacac ctatagctgg
     2401 acgcccgaca tcacgttcga caccagcggg ggtacgccga gcggcgccag ctactacgct
     2461 gtggtctcac tccccaccgg cgccccctgg ctggagttag agtgcgattg ggcggggggg
     2521 gggcggaaca aatcatattt cctgtgcggc ggcccgaaag tccccgagga taagggcacg
     2581 acggcgacgg gcctctttcc cttcgccatc aagctgcgga acgaactgaa gggcaccgat
     2641 gagacgctct ttaccggcaa ggtcaagatc gaaaaagcgc cgggcgacaa cggcacgccc
     2701 gcggagcgcg ccaaaaaatc cttcttctac cccaacatgg attgggcgct gcccgtcggc
     2761 tacgtttatt acagccccgc ggacaaccac ctctacaccg ggttctgggt gcgcgggagc
     2821 acctacaagg tagccccgca cgtgttctat cagggtaagg agataggatt ggtgggggac
     2881 tgcgattcca acgtcgaggt cggggatgcg tacatacgct cggtcaagcc cgcccccttc
     2941 tggaagtacg tcgagtgcga actgctctcc gttaaaatca aggacgaaag ggatgagttc
     3001 agcctgatgt cgaaccccgg cgagtacgag atcaaggtgc tgtggaacga cgagctgtcg
     3061 cgctcgatca agttcacggt cggccgcgac ggccagctcg ccggcggccc ccccctactc
     3121 tacagggtgc gggacttaga cggggaacgg caacccggcg tcatcgtgcc cgtcgcgatc
     3181 ctcgacgcac aggacggccc gtgggacaaa aacgcctggc ggaccggcgc cttctacggc
     3241 aacccgccga cgggcttctc gccgccgcag tagcccggtt gagtgttggg gaggcagact
     3301 caccacagag acacagagat cgcaaagggg gcacagggag ttgaattcct caacattccc
     3361 acaggccagc tcgtgggcgg gcttgtcgaa ggtgcctttg taaattgaga gacacgcccc
     3421 agagacacaa ggggcgccgc tcatccattt tcaagataga aggagcatcc agatgggaaa
     3481 gcgtatcttc ctcgcgctcg cgttatcgag ctgcgtcggt ttcggcctac acacggccgt
     3541 ggcacagttg cccggcggcc tcccccgcat cccgcgaccc ggcaggccga aggccacgcc
     3601 gaccccggcc ccgaccgcgc agcccgcgcc gtcgggcgag acacggcccg ccgcgcagcc
     3661 gcagccggac gcgcccgccg cgacgaatcc cgccccgccc gcggcggccc cacgtccgca
     3721 ggcgggaggc atcacgctaa ccaagaccct gatccggttc tggccctata cggtcacctc
     3781 ctaccagggg aactataaca atgtctggag ctggttcccc acggtcagat tcaataccga
     3841 gggggggccg ctgcccagcg gcgggcaata ttacgtcgag gtcgcacagc ccggcggcgg
     3901 cgcgtgggtg aagctcaaat gcgttaacac cacgattggc ggcccgacct tcgagtgcgg
     3961 cgaccgtaac gatctggagc agagcggcac gctcgcgacc ggcgtcttcc ccttcgtcat
     4021 caagatgcgc aacccgcttg agggcaccga ggggaagacg ctcttcaccg gcagggcgaa
     4081 ggtcgaaaag gtgctctcga acgagcacgg ccctcaagcc gttaagaagt tcgtttacta
     4141 cgccaaccag gactggaatt tgcccatcgg gcaggtctac atcgactccg atgaccatct
     4201 caacgtcagg ttctggatcc gcggcgaggg cgcagggggc cttgaagcac atccgatgta
     4261 ccggggcgag gaggtgggac ggtaccaaga gcacggctac cagtacggcg gggccagctg
     4321 ctcgcccgac atcgagtact tcccaacaca gagcgcgggt gcgggggcgc cgcaggaggc
     4381 gaagtgggcg cgcgtcaact gcaaattcgg gagcgtagtc accaagccgg ctaagaacgt
     4441 cccgcaatgg cacgtgctcg gcgcgaatcc cggggagtac gagatcaagg tgctacgcaa
     4501 caagaggctg gcgcgctcga tcaagttctc catcggaccc gacggcaaga tcgttgataa
     4561 ccgcatcgcc gccaacagtg ggatgggggc gtgggacaac cacatcttag tgcccgttac
     4621 tattatcggc gagcaggacg gaacgtggga caagaacgcc tggaagaccg aagccttcta
     4681 cggccacccg ctcgccggct tcacttggcc gccgcagtaa gacaaagggc gccgccttaa
     4741 ttgattaagg cggcgcccac gccagcgtca gttaagcttc cacaccgggt cggcgcgcgc
     4801 cccgggcgcg acggcgcggc acacaggggg ctggccgggc aatcatctgc gtctcagcgc
     4861 aggcgctgcc tgacaacggc atgcacccga ccgcgaatca gcgggcctct tatcgtgaac
     4921 ttggctgtac gccggggccg tcgcccgtga gggtagggcc ggcggaacag ggccggcgtt
     4981 tgagtcgtca ggagttgtcg taaggtaatc tccaataacg aaatagtcgc tattgacatc
     5041 tatttcgcgg aagcatatct tgcccgcgtc aagggccgtt tgacagccgg caggagtaat
     5101 ctggtcagcc tcacgttcta aattacaaac aggtggcaaa aggggaggcg accaaagggc
     5161 gaatcctaac gcgtcagaga tggatgggac ttaacacgtt ccgtcaggga ggtgtccgac
     5221 atgaagttgt tgctcatcgc gccggaaggt ctggaggttc agggcttcaa aggttccaag
     5281 cacgcccacc acctcaacct ggctttgatt gccgcactcg ccacacccta cttcgaggat
     5341 atcaagatcg tcgaggagga gtttgagacg ctcgacttgg acgagccggc cgacctcgtg
     5401 ggcatcacca tgatgacctg ccaggccgag cgcggctacc aactggccga ccactttcgc
     5461 aagcggggcg tccgcaccat ctgcggcggc agccacgcga ccttcatggc cgaggaatgc
     5521 ggcgagcact tcgacgccgt cgtggtcaac gaggtcgaga tgatctggga agagatcatg
     5581 gctgacttca tggccgaccg gctcaagagc gtctaccaca ccgaccagct cctcgacctc
     5641 agcctgctgc ccatgccgcg caaggagctt ttccgcgaca actggaaggg caagttcgcc
     5701 gacgtgatcc aggccggccg cggctgcccg ctcggctgcg agttctgcac ggtcacgaat
     5761 atgtacggca agacgttccg cacccggccc gtcgagcaca tcgtcgagga gatcaaacgc
     5821 ttcaagtcca agcgcctctt cttcgtcgat gacaacatct tcctgtcgcg caactacgcc
     5881 tacgagttgt gcgaggcgct catcccgctc aagatcgact ggggcagcca gggttcgatg
     5941 gagctgatct gccgcgacga agagctgctg aagctggcca agcgttcggg ctgcatcagc
     6001 ctgttcgtcg gcatcgagac cgtggatcag gagaccctga acaagaacca caagcagttc
     6061 aacaaggtgc agaagtacga ggagaacatc cgcaagatgc accgcgccgg catcaacgtc
     6121 atcggctcct tcatcttcgc cttcgaggag gacacggccg agtgcttcag caaggtctac
     6181 gacttcgccg tccagaaccg cctctccatg gtcaactgcg gcatcatgac gcccttcccc
     6241 ggctcggtca cttatgacaa ggcccggcgc gacaagaaga tcgtcgattt tcgctggggc
     6301 cagtacaccg gctctaacct cgtgtggcgg caccccaaca tgagccgggc ggaagtcgag
     6361 gcgcgctacg accgcttccg ccagcagttc tactcgtggt cgtcgattct caaacgcgcc
     6421 tggcgcaacc gcgcgcaccc gctctattac tggagcatga acctgcacct gtggtggctc
     6481 taccacgtcg agccgcgtcg agcgccgcgc tgcctgccgc cgtgaggccg tgcctctctt
     6541 tggcttttat agatctgtgt gcgacttcag tccggggcgg gctgcgaggg ggacacggat
     6601 gagacggatt aaaacggatg aagacgaatc gcatccgtgc tcatccgccc gatccgttta
     6661 atccgtgttc cccacccggc gcgcaacaca gacgcacggc gggactcttc acaccaactt
     6721 attttctcaa cgcttccgaa gggaagtgat agctcctgac tctgctacct gctaccatgc
     6781 gctctaccgc ctgatcgtta gacccggcag cccgcaagag ccgaatgtag atgcaaccac
     6841 ccactcctga aaactcgccg cgagtgacgc ccccacccga gacgttagtg gaggcgctga
     6901 atcacgtcgc gcacgagcac gagcgcggct tccacttcgt ggggttcgac tcgcgcgagc
     6961 agttcttctc ttatcagcat gtccacgcgg aggcgctgcg gcgtgcggcg cacctctccg
     7021 cgctcggcct gcggaaaggt gatcggctgg cgttggttgt gtctgacggg ggcgagttcg
     7081 tcatgagttt tttcggcgcg gtgatggccg gcgtggtggc cgtgcccatc tcttcgccgt
     7141 tcacgcagaa ggcgccggcc agctacgtcg agaacgtggc gcgcatcgtc cgggcggcgg
     7201 gcgcgagaat gcttctgacg acggacaaga tcaggcccac ggtgcagggc gtctcggaac
     7261 tggcaggtgc gctcgaacgc ttggtcacgg tagaggaagc gtttgccggg gagactcccg
     7321 cgttcgagcc gccccgcgtg cgacctgaag acctttgttt ttttcagttc acctcgggca
     7381 gcacttcctc acccagaggg gtgatgatct cgcacgccaa cctgatggcc aacgtcacgg
     7441 ccttcatggg gccgttcggc atcgacagcc ggcccggaga tgtcggcgtc agttggctgc
     7501 cgctttttca cgacatgggg ctgatcggat ttattctcgc gccgctggcc actaatcgac
     7561 aggcggtgat tatttcgacc accgcctttg cgcgcgaccc gcgcatctgg ctgaggacgc
     7621 tgcataaata ccgtgggagc gtcacctacg ccccgaactt cgcctacgcc ctcacggcga
     7681 aacgcgtgcg cgatcaggat ttggaggagc tggacctctc ttgcgtccgc gtcgccggct
     7741 gcggcgcgga gccgattcac gccccgacac ttcgcagctt tgccgagcgg ctggccccgg
     7801 tcggatttaa ctccgacgct ctgctgccca gctatggact ggccgaatcg actttggccg
     7861 tcacccttca tcgcgcgggc gcgccgctgc gaaccgataa gatagacgcc gacgcgctca
     7921 agtggggccg cgccgcgccg gccgacgagc agacgaaaca ggtgtcggag gtcgtctcct
     7981 gcggcgactg tttccccgac catcaagtcg tgatcgttga tgaggacggt gctgttttgg
     8041 acgagcggcg ggtgggcgaa atccttgcgc gggggccgag catcgcgcgt ggttactaca
     8101 acaccccgga ggcgacccgt gcgacgtggc aggacgggtg gctgcacacg ggagaccgcg
     8161 gctacatcgc cgacggagag ctttttgtct gcgggcgcat taaagacctc atcatcattc
     8221 gcggggccaa tttctatccg cacgacatcg agtcggccgt gcgcgatctg ccgggcatcc
     8281 ggcacggcaa cgtcgcggcc ttcggcgtga tggacggggg tcaggagaag ttgaccgtga
     8341 ttgccgaaac gaacgggcgc gatgattcgg agttgggcaa gttgatcgcc gagcgcgtcc
     8401 gcgaggccgt cgggcttgaa gtccaccgtc tggtgtttgt gccttcaggg actctgccca
     8461 aaacttccag cggcaaattg cagcggcaca aagttaaaga actcttcgag aggggacaac
     8521 ttaccgccgc cgacgctcag gccagaagag cagcgcccgg cgctacgggg cagtcgcaag
     8581 agatttccgc cgggggcgcg ccgccgctcc tcggcgtccg gcagttcgcg ggagacttag
     8641 aagagcaggc gggatgagcg aattacaatt acatagacat cagggaaagt agaggggcac
     8701 tgtccttgtc aacgcgagaa gacatagcgg ttacttacga cgtggcgaac gatttctttc
     8761 gcctgtggct cgacgagcgc atgatgtact cctgcgcgct cttcgagggc acggacgatc
     8821 tcgaaacggc tcaactcaat aaactccgtt acttccacga cggcgtgcgg gtcactcccg
     8881 acaagcgcgt gctcgacatc ggctgcgggt ggggaggcaa tctggagttt ctcgcgcgcg
     8941 acatgggcgt gagggacgcc gtcggcatca cgctttcgga agcgcagttt gaagaagtca
     9001 gggcgaaggc cgtccccggc gtgtcggtcg agtgtgtctc ttacaaggat taccggccgg
     9061 agcagaagtt cgacgccgcg atcagcatcg gcatgttcga gcacgtcgcc acgccggagc
     9121 aggcgcgctc gggcgaacaa atcgccgtct accgcgacta cttccgacgc gtctgggaat
     9181 ggacgaatcc cggctcgtgg ttcggtctcc agagcgtcat cagcctgcgc atcccgcgcg
     9241 accggcgcga cctgcaccgg ctcggctggg gcacctccac gattttcccc ggcgcgatca
     9301 ccccgcgtct ggaggccatc atggcttcgg tcaatcctta ctgggaggtc atggaggtga
     9361 agacgcggcg ggagcattac gcccggacga gcgccgaatg gctgcggcgg ctcaagggca
     9421 acgaggcggc gattcgcgcc cgctggggcg acgagttgtt tgccgactac gtgaagtatc
     9481 tgggcgggtg cgtgatggtg tttgataaag gctaccaatc acttgtacaa atactcctgc
     9541 gccgcacgga tgagcgcgcc gattttgaca cactcgccaa gatggaggga aaataagtga
     9601 cgaacctcga ccacggggac ttgatcaaag aggcgctggc gttcaccgcg ccggaagaga
     9661 tgccgcagga gttctccccc gacagcacgc tcggtgagat gggcatcagc tccatcaccg
     9721 cgctggagat ggccgggtac atcgaggaaa aattatccat cgtgttcccg gacgacgaat
     9781 tgtctcaggt caacaccctt cgcgatttcg accagttgat ccgccgtcac acactggcaa
     9841 cgcaggaatg agtcgtaaaa ggagcaggcg atgagcgtat cccgcgcata ccacgagcag
     9901 atgtaccgca tctaccacga gttcttcgac gtggccgaga cgaagcggcg gtggcacccg
     9961 ttccgcgacc tgccgtggga gaagatggac aagtcgaaga acacggaaga gctggccatc
    10021 tgtctggaaa cattttgcgg cgtcgagttg tacgtccccg actacacggc caacgccttc
    10081 aacatgacgc gcacgatctt cggtcaggcg tggttcgagg ccaactgggg ctacgaggaa
    10141 tcgaagcacg gcctgagctt ccgcgaatac ctcgtccagt cgggcctgcg cacgcccgag
    10201 cagtacgcgc agtatgaaga ggagattttc agcaaggtct ggcggctgcc gttcacgacg
    10261 cggcggcaga tgacctgtta cggcgttctt caggagtcgg cgacttacct gatttacgag
    10321 gcgcagaaga agcgggcgca gcaggaagaa aacgagttgc tggagaagct cttcttcttc
    10381 atcagccgcg acgaggcggc gcaccggggc ttttatcaga aggtgctcgt ctacgagctg
    10441 gaggaagacc gccccggcac gctggccgac ctcgcacacg tcatctcaca cttcgagatg
    10501 ccgggggtgc cgctgattcc cgaattccag aaacgcctgc tcgtcaacgg cgtcggaatc
    10561 acatctcagc acttcttaca gcgcggaatc ttcccgctcc tcaaagcgat tggaaccagc
    10621 cggagcgagc tgataaaaat actcggccag cagaaggcca gagaagctga aagaaagaag
    10681 ggcgaggaga tgcctccggc tgttcccctc gcggaagccg aaccggcgat ggcgtcgaag
    10741 tgaaagtcga ggtgacggcc ggaagggaag acgcggccgt tccgccccgg ctcgcatggc
    10801 tatcattgtt cggtgcgccc gacgcctgag cggccgtcgt cgtccacctt cgacacgccc
    10861 ggcctcgcct gcttctccct cacggccctc gccctgaagc aacttcacta acactccttc
    10921 ggcaagcttc gcgcgcgcgc gaggcggccg ggtgagaggt gatatatttc gcagcagccg
    10981 gttcaaagag agatcattca aatcgtcgcg ggagacggcc gtgtcttgtc ggcgcgttgg
    11041 tgggcggggg cgcgggccgc ggagcggtgt gtggtcttca tcccggcgct ggccgccccg
    11101 caggagtact gccgattctt cgccgccttc ctcgccaggc aagggtgggg ggtgctgacg
    11161 ttcgactatc gcagcgtcgg cgcgtcgaag gattccgcgt cggacgagac ggccactctg
    11221 gacgattggg cgcggctcga catcccggcc gccgtctccg aggcgcgacg ccgaagcggg
    11281 gcacagtttc tcgccgtgtt cgcgcacagc atcggcggcc aactgttggg gcagagtccg
    11341 gcccgcaggg agattgacgc cgccctgctc atcgcggcgc agcgcggcat ccccaagctc
    11401 tttaagggac gcgggcggct gcgcgtctct tacgcgtatg cggtcttcac gatcctgatt
    11461 cggtctttag gttatctgcc ggtctccaaa ctcacgttgc cggagcggtg ttccgctcag
    11521 gcgttacttc agtggatcag gtgggggcgg acgggcgtct ttacggacgc cagtggcgtc
    11581 aacgtggagc cgggctacgc cgaatatcgt gggccgctca cggccgtccg cgtcgccgac
    11641 gacgactacg cgcccgcgcc cgccgtcgaa gccttaacgg ctctgtacgc aagctcgccg
    11701 acgcggcgcg agaccatcga gcctcagaag tacggcccca agcccgtcgg ccacttcggc
    11761 ttctttcacc cccgcgcgcc gcgcgccctg tggtcggacg tggaatcatg gctgcgacga
    11821 atggaggaag aggcgcgttc cttgaagtct tagagcgacg ttcacttgag tgaacaggga
    11881 atagattcac cgcggaggcg cggaggacgc agaggaagcg cggaggcttg acgctctcac
    11941 tcaacgtcga gtctaaggct ccagtctctg cgtgaacttt gcgtcctccg cgcctccgcg
    12001 gtgaatctac ttcgctctca ctcaactgaa agcggctata gttaccctgt ggcaggcggc
    12061 ggagacattg taccgggccg ggcgggtcgc catgcaaacg gagtctggaa gaggcgcatg
    12121 cagtcaacac ccctcatctt tgaagagatt gaaaaagcgg tcagcctcta cccggagcac
    12181 acggcgttgt tgcagttgct cgacggcgag ggggaagtcc gctacacctt ccggcagacc
    12241 ctgaggctgg caaagaattt aagtcgggcg cttaagggaa gaggattcgg caagggcgat
    12301 aaggtcgtgt tctgggcgcc gctcagccct cactgggtca tcgcttatct gggagttctc
    12361 tacagcggtt gcgtcgttgt gcctctggac gtggagtacg gaagcgatga actggcgttc
    12421 gtcctcgccg aactcgactc ccgcctcatc ttcacgactc gcgaaaaact cccgctgctg
    12481 cggagcgcga cggaagggcg tccagcggag atgaccgtcg tctctttaaa cggagtcggc
    12541 gaggcggacg ccgctactgc tcaggtggag gagttgttcg aggagagcga ggccgacccc
    12601 gcagtgcctc ccgtctcgcc ggaggacgtg gccacaatct tctacacgtc cggcaccacg
    12661 gggaagccca aaggggtggt catcgagcac ggggccgtgg ccgccggcgt cttgtcgctg
    12721 aagcagtaca tcgagttcat tcccggcgac aacgtcctcg ccgttgtccc ggcgcat
//
LOCUS       DASVES010000430         7161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_663924, whole genome shotgun sequence.
ACCESSION   DASVES010000430 DASVES010000000
VERSION     DASVES010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663924"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF240_17630"
     CDS             <1..406
                     /locus_tag="VF240_17630"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7177083.1"
                     /translation="SVARSRFQMLLLTIFAAVALALAGVGLFGVMSYTVTQRTHEIGI
                     RMALGARAGDILRMVVRQGMVLVGVGVALGLAGAFALTRFISAMLYGVSATDPATFAG
                     VAVLLGLVALLACYLPARRATKVDPMVALRYE"
     gene            485..2923
                     /locus_tag="VF240_17635"
     CDS             485..2923
                     /locus_tag="VF240_17635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177084.1"
                     /translation="MGTLLQDIRYGFRMMWKRPGFTAIVVLTLGLGIGANTAIFTVVN
                     AALLRGLPYEEPERLVHLFETTPQKNFGRREASYPDFLDWRQNQTFEGLAAYSGGGGF
                     ALAGADGSELVESGRVTANFFDVLGVRPALGRTFREGEDEAGAERVVVLSHGAWQRRF
                     GGDAEVVGRTLTLSGESYTVVGVLPPQFQFAARGSAEMWAPWRPGEGQRTRRFMHWVN
                     VVGRIKTGVSQEQARAELANIARRIETEHHESHAGTNIVLVPLQEQFVGQLKPLLLVL
                     LGAVGFVLLIACTNVANLLLVRGAARQKEFAVRAALGAGRRRLVRQMLAESLLLALVG
                     GALGLVLAQWGVDALVAAIPQQRLNSMPYLRGLGLDTGTLVFTAALSVGVSLLFGLAP
                     AFQASKLDLNATLKEGGGKGAAGASRSRLRSALVVTEVALSLVLLVGAGLMLKSLSRL
                     LEVDPGFDTKNLLTFRLALPATEYGDYDRVTNFHRQLLSRLEALPGVRGVATVGSMPI
                     LGGNTTRFYAAGQSVPAPGTETEANLRNISAGYFRVVGMPLVSGREFSERDTKDSPPA
                     IVVNQTLAARVFPGEEAVGQRLVFTGDGGPPVEIVGVVGDERINGLDAAVTPVVYYPY
                     LQDTPPSTGTVVLVRTSGDASAMAEVVRGETRALEPGALFFGARTMEGIIADSPATFM
                     RRYPTLLIGVFAAVAFLLASVGIYGVVSYTVSQQTHEIGVRMALGAQTRDVLRLIVGR
                     GMALALVGVGVGLVGAFALTRLMSSLLFGVSPTDPQTFAVVAALLVAVALAACLVPAR
                     RATRVDPMTALRYE"
     gene            3030..5513
                     /locus_tag="VF240_17640"
     CDS             3030..5513
                     /locus_tag="VF240_17640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177085.1"
                     /translation="MKNLWQDVRYGARVLWKRPGFTAVAVLALALGIGANTAIFSVVN
                     SVLLRPLPYPQPERLVRVTVANVQRGTRDNPAAYLDFADWQQQTNSFEALAAYSSGSA
                     SLTGDGSAPEQLNGVVTTPDMFKVMGVSPALGRTFTAEEAARIAARGEKKEGAEGQGS
                     GAAAAPQVVVIGHGLWQRRFGGDPSIVGRDVMLDNAPSTVVGVMPEGFRYPLDYDEVE
                     FWAPLDTSNEFMRHRGASFLDVVARLKSGVSQEQASAELAAVSERLAADYPNTNRTRR
                     AETQSLHERLVGDIRPALLVLLGAVGCVLLIACANVANLLLVRAASRQKEFAVRTALG
                     ASRWRIMRQLLTESFLLSLAGGVAGLLFALWGVDLLLAVVPADIPRMGGISTDARVLV
                     FTLGATLATGLVFGLAPALQASKTSLNESLKEGERGSTASRNRLRGGLIVAEVALSLV
                     LLAGAGLLVKSFWKLTAVNPGFNPEGVLTATMTLSDNKYEEDDQQREAYRRALERVAA
                     MPGIDSAGLISPLPMSGNSIGTNFTVNNNTFDAPENVYRGERKAVSADALRTLGIPVV
                     RGRAFTERDGEGAPRVFLVSEELARKCFPDGDALGRQMSMRDLGGISTGEIVGIVTDV
                     KYEGLDAETPPAYYVSYLQTPSNGLSVVVRARAGTDAAALAPTLRAAVLEADKDQPLA
                     EVRTMSAMLANSLARERFQMLLLSIFAGTALLLAAVGLFGVMSYTVAQRTHEIGIRMA
                     LGAQTRDVMRLIIGRGMALTLAGVGVGLVGAFALTRLMSGLLYGVSAHDPATFALVTA
                     LLVIVAFLSCYLPARRATKVDPMVALRYE"
     gene            5603..>7161
                     /locus_tag="VF240_17645"
     CDS             5603..>7161
                     /locus_tag="VF240_17645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177086.1"
                     /translation="MGTLGRDVRYAVRMMWKRPGFTLVAVMALALGIGANTTIFSLVN
                     ALLLRPLGGVEDPSRLVAVFTSDFSSGLYGGSSYPDYLDLRDQCESFAGLAAFDDSVA
                     NLNADDEAERLRGQSVTGNYFNVLGVGAAAGRLLTPADDEASAAERVVVISHDLWRRR
                     FGSDAALVGRSVLLDGHPHTVVGVAAKEFRGTRLGSTGPAFWAPFNQPDVAARSRGSR
                     GIGIVGRLKVGATLEQAEAEVATLAARLAQSYPKTNLGTLARPEEPRPMSVAQEGRIG
                     PGQQRAVWTASALLLAVVGFVLLIACANVANMMLARASSRRREIAIRLALGAGRLRIM
                     QQLLTESVLLALVGGVAGLLIALWTADLLPTFFPSEDAAALDFSLDWRVLGFAAAVSL
                     VSGVLFGLAPALQATRPDVVVALKDDAGAGGAGPRTVNLRNALVVAQVALSLVLLIGA
                     GLFLRSLRNAASADPGFDASGVLIARLELRGDEAKAERGQLFYRELQERVSSLAGVRS
                     VGLTRIVPLSGG"
BASE COUNT          996 a         2484 c         2458 g         1223 t
ORIGIN      
        1 ctcggtcgcg cgcagccgct tccagatgct cctgctcacc atcttcgcgg ccgtcgcgct
       61 ggcgctcgcc ggcgtgggcc tcttcggcgt gatgtcgtac acggtgacgc agcgcacgca
      121 cgagatcggc atccgcatgg cgctcggcgc gcgcgccggc gacatcctgc ggatggtcgt
      181 gcggcagggg atggtgctcg tcggcgtcgg cgtcgcgctc ggcctcgccg gggccttcgc
      241 cctgacgcgc ttcatctcgg ccatgctcta cggcgtgtcg gccaccgacc cggcgacctt
      301 cgcgggcgtc gccgtgctgc tcgggttggt cgccctcctc gcctgctacc tgccggcgcg
      361 gcgcgcgacg aaagtcgatc cgatggtggc acttagatac gagtgataag tgatgagtga
      421 tgagtgataa gaaagagcaa tctgtctttt ctcatcactc atcacttccc gaagggattg
      481 agtcatggga acactgctgc aagacattcg ctacggcttt cggatgatgt ggaagcgacc
      541 cggcttcacg gccatcgtgg tgctgacgct ggggctgggc attggcgcga acacggccat
      601 cttcacggtg gtcaacgcgg cgctcctgcg gggcttgccc tacgaggagc cggagcggct
      661 ggtgcatctg ttcgagacca cgccgcagaa gaacttcggg cggcgcgagg cttcgtatcc
      721 tgacttcctc gactggcggc agaaccagac gttcgaggga ctggcggcct actccggcgg
      781 cggcgggttc gcgctcgccg gggccgacgg ctccgaactc gtcgagtcgg gccgcgtcac
      841 cgctaacttc ttcgacgtgc tcggcgtgcg ccccgctctg ggccgcacct tccgcgaggg
      901 cgaagacgag gcgggggccg agcgcgtcgt cgtgctcagc cacggcgcgt ggcagcgtcg
      961 cttcggcggc gacgcggagg tggtcggccg cacgctcacg ttgagcggcg agtcgtacac
     1021 ggtcgtcggc gtcttgccac cgcagtttca gtttgcggcg cgcggctcgg ccgagatgtg
     1081 ggcgccgtgg cggccgggcg aggggcaacg cacgcgccgc ttcatgcact gggtcaacgt
     1141 cgtcggcaga atcaagacgg gcgtcagcca ggagcaggcg cgcgccgagt tggcgaacat
     1201 cgcgcggcgc atcgagacgg agcatcacga gtcgcacgcg ggcacgaaca tcgtgctcgt
     1261 tccgttgcag gagcagttcg tcgggcagct caagccgctg ctgctcgtgc tgctcggggc
     1321 cgtcggcttc gtgctcctga tcgcgtgtac gaacgtggcg aatcttttgc tcgtgcgcgg
     1381 cgcggcgcgg cagaaggagt tcgccgtgcg cgcggcgctc ggggcgggcc gtcgtcggct
     1441 ggtgcgacag atgttggccg agagtctgct gctggcgctc gtcggtgggg cgctcgggct
     1501 tgtgctggcg cagtggggcg tggacgcgct cgtcgccgcc atcccgcagc agcgcctgaa
     1561 ctccatgccc tacctgcgcg gtctcggtct cgacaccggc acgctcgtgt tcacggccgc
     1621 actctccgtc ggcgtcagcc tcctcttcgg cctcgccccg gccttccagg cgtcgaagct
     1681 cgacctcaac gcgacgctca aggagggcgg cggcaagggc gcggcgggcg cctcacgcag
     1741 tcgcctgcgc agcgcgctcg tcgtcactga ggtggcgctc tcgctcgtcc ttttagttgg
     1801 cgcggggctg atgttgaaga gcctgtcgcg gctgctcgaa gttgaccccg gcttcgacac
     1861 gaagaacctg ctcacgttcc gtctcgccct gcccgccacg gagtacggag attacgaccg
     1921 tgtcaccaac ttccaccggc aactgctctc gcgcctcgaa gccctgcccg gcgtgcgggg
     1981 cgtcgccacg gtcggctcga tgccgatact cggcggtaac accacgcgct tctacgccgc
     2041 cgggcagagc gtccccgcgc cgggcacgga gacggaggcc aacctgcgca acatcagcgc
     2101 cggctacttc cgcgtggtgg gcatgcccct cgtctctggc cgcgagttct ccgagcgcga
     2161 cacgaaggac tcgccgccgg ccatcgtcgt taaccagacg ctcgccgcgc gcgtcttccc
     2221 cggcgaggag gcggtcgggc agcgtctggt ctttacaggc gacggcggcc cgccggtgga
     2281 gatcgtcggc gtcgtcggcg acgagcgcat caacgggctg gatgcggcgg tgacgccggt
     2341 cgtctattac ccgtacctgc aagacacgcc gccctcgacc gggacggtcg tgctggtgcg
     2401 taccagtggc gacgcgtccg cgatggcgga agtcgtccgc ggggagacgc gcgcgctgga
     2461 gccgggggcg ctcttcttcg gggcgcggac gatggagggg atcatcgccg actcgccggc
     2521 caccttcatg cgtcgctacc cgacgctttt gatcggcgtc ttcgccgccg tggccttcct
     2581 gctcgcctcg gtcggcatct acggcgtcgt ctcctacacg gtcagccagc agacgcacga
     2641 gatcggcgtc cgcatggcgc tgggcgcgca gacgcgcgac gtgctccggc tcatcgtcgg
     2701 gcgcgggatg gcgctggcgc tcgtcggcgt cggcgtcggc ctcgtcggag ccttcgccct
     2761 gacgcggctc atgtcgagtc ttcttttcgg cgtcagcccg accgacccgc aaaccttcgc
     2821 ggtcgtcgcg gcgctgctcg tcgccgtggc gctggcggcc tgtctcgtcc ccgcgcgccg
     2881 cgccacgcgc gtcgatccga tgacggcttt gagatacgag tgataagtga tgagtgataa
     2941 gtgatgagtg ataagtgata agaaagagca atctgtcttt tctcatcact catcacttat
     3001 cactcatcac ttctgaaaga gatttcgtga tgaaaaactt gtggcaggac gtgcgctatg
     3061 gggcgcgggt gttgtggaag cggcccggtt ttacggccgt ggcggtgctc gcgctcgctt
     3121 tggggatagg ggcgaacacg gccatcttct cggtggtcaa ctcggtgctg ctgcggccgt
     3181 tgccgtaccc gcagccggag cgtctggtgc gcgtgactgt cgcgaacgtc cagcggggga
     3241 cgcgcgacaa tccggccgcg tatctggact tcgccgactg gcagcagcag acgaactcct
     3301 tcgaggcgct ggccgcatac agctctggga gcgcgtcgct gacgggcgac ggtagtgcgc
     3361 cggagcagtt gaacggcgtc gtcaccacgc cggatatgtt caaggtgatg ggcgtctctc
     3421 ccgcgctcgg ccgcaccttc acggccgagg aagcggcgcg catcgcggcg cgcggcgaga
     3481 agaaggaagg ggccgaaggg cagggcagcg gcgcggcggc agcgccgcag gtggtcgtca
     3541 tcggccacgg cctgtggcag cgtcgcttcg gcggtgatcc atcaatcgtc ggccgcgacg
     3601 tgatgctcga caacgcgccc tcgaccgtgg tcggcgtgat gcccgagggc ttccgctacc
     3661 cgctcgacta cgacgaggtg gagttctggg cgccgctcga cacctcgaat gaattcatgc
     3721 gacaccgcgg cgcgtcgttc ctcgacgtgg tcgcgcggct taagtccggc gtgagccagg
     3781 agcaggcgtc ggccgaactc gccgccgtct ccgagcggct ggcggccgac tatccaaaca
     3841 cgaaccgcac ccggcgggcc gagacgcagt cgctccacga gcgtctggtc ggcgacatcc
     3901 gccccgcgct cctcgtgctg ctcggcgcag tcgggtgcgt gctcctcatc gcgtgcgcta
     3961 acgtcgccaa cctcctgctc gtgcgcgcgg cctcgcgaca gaaggagttc gccgtccgca
     4021 cggcgctcgg cgcgtcgcgc tggcgcatca tgcggcaact gctgactgag agttttctgc
     4081 tctcgctcgc gggcggggtg gcggggctgc tcttcgcgct gtggggtgtt gacctcctgc
     4141 tcgccgtcgt acccgccgac atcccgcgca tgggcggcat cagtacggac gcgcgcgtcc
     4201 tcgtcttcac gctcggggcc acgctcgcca ccggcctcgt cttcggactt gcgcccgcgc
     4261 tgcaagcctc gaagacgagc ttgaacgaat cgctcaagga gggcgagcgc ggctcgaccg
     4321 cctcgcgcaa ccgcctgcgc ggcggcctca tcgtcgccga ggtcgcgctc tcgctcgtgc
     4381 tcctcgcggg cgcgggcctg ctcgtcaaga gcttctggaa gttgacggcc gtcaaccccg
     4441 gctttaaccc cgagggcgtg ctgacggcga cgatgactct ctccgacaac aagtatgagg
     4501 aggacgacca gcagcgcgaa gcctaccggc gcgcgttaga gcgcgtggcg gcgatgccgg
     4561 gcattgactc ggccggactc atctccccgc tgccgatgag cggcaacagc atcggcacca
     4621 acttcaccgt caacaacaac accttcgacg cgccggagaa cgtctaccgc ggcgagcgca
     4681 aggccgtcag cgccgacgcc ctgcgcacgc tcggcatacc cgtcgtgcgc ggccgcgcct
     4741 tcacggagcg cgacggcgag ggcgcgccgc gcgtcttcct cgtcagcgaa gagctggccc
     4801 gcaagtgctt ccccgacggc gacgcgctcg gccgccagat gtcgatgcgc gacctcggcg
     4861 gcatctcgac gggcgagatc gtcggcatcg tgaccgacgt gaagtacgaa ggtctcgatg
     4921 cggagacccc gccggcctac tacgtttcgt atctccagac gccgagcaac ggcctctccg
     4981 tcgtggtgcg cgcccgcgcc ggcacggacg ccgcggccct cgcccccacg ctgcgcgccg
     5041 ccgtgcttga ggccgacaag gatcagccgc tcgccgaggt ccgcacgatg agcgcgatgc
     5101 tggcgaactc gctggcgcgc gaacgcttcc agatgctcct gctcagcatc ttcgccggca
     5161 cggcgctcct gctcgccgcc gtcggcctct tcggcgtgat gagctacacg gtcgcccagc
     5221 gcacgcacga gattggcatc cgcatggcgc tcggcgcgca gacgcgcgac gtgatgaggc
     5281 tcatcatcgg gcgcgggatg gccctcacgc tcgccggcgt cggcgtcggt ctcgtcgggg
     5341 ccttcgccct gacgcggctc atgtccggcc tgctctacgg cgtgagcgcc cacgaccccg
     5401 cgaccttcgc gctcgtcacg gcgctcctcg tcatcgtcgc cttcctctcg tgttacctgc
     5461 ccgcgcggcg cgcaacgaag gtcgatccga tggtggcgct gagatacgaa taataagagg
     5521 tcagaggtca gaggttagag gtcagtaaaa actgtcttca ctaacctctg acctctaacc
     5581 tctgacctct ctggagattg ttatgggaac acttggccgt gacgtgcgat acgccgtgcg
     5641 gatgatgtgg aagagaccgg gcttcacgct ggtggcggtc atggcgctgg cgctcggcat
     5701 cggcgccaac acgacgatct tcagcctcgt caacgcgctg ctgctcaggc cgttgggcgg
     5761 cgtcgaagac ccgtcgcgcc tcgtggccgt cttcacgagc gatttcagca gcggcctcta
     5821 cggcggctcc tcttaccccg actacctcga cctgcgcgat cagtgcgaat ccttcgctgg
     5881 cttggccgcc ttcgacgact cggtggcgaa tctcaatgcg gatgacgagg cggagcggct
     5941 gcgcgggcag tccgtcaccg gcaactattt caacgtcctc ggcgtgggcg cggcggccgg
     6001 ccgcctgttg acacccgcgg acgacgaggc gtcggccgcc gagcgcgtcg tcgtcatcag
     6061 ccacgacctc tggcggcgtc gcttcggctc cgacgcggcc ctcgtcggcc gcagtgtcct
     6121 gctcgacgga cacccgcaca cggtcgtcgg cgtcgccgcg aaagaattca gagggacgcg
     6181 cctcggctcg acggggccgg ccttctgggc gccgttcaac cagccagacg tggcggcccg
     6241 gagccgcggc agtcgtggga taggcatcgt cgggcgtctc aaagtcgggg cgacgctcga
     6301 acaggccgaa gccgaagttg cgaccctcgc cgcgcggctc gcccagagct atcccaagac
     6361 caacctcggc acgctcgcgc ggcccgaaga gccgcggccc atgagcgtgg cgcaggaggg
     6421 ccgcatcggc cccgggcagc agcgggccgt gtggacggcc tcggcgctcc tgctcgcggt
     6481 cgtcggcttc gtgctgctca tcgcgtgcgc taacgtcgcc aacatgatgc tggcgcgggc
     6541 ctcttcgcgc cggcgcgaaa tcgccatccg tctcgcgctc ggggccgggc gtctgcgcat
     6601 catgcagcaa ctcctgaccg agagcgtgct cctcgcgctc gtcggcgggg ttgccggcct
     6661 gctcatcgcc ctctggacgg ccgacctcct gccgaccttc tttccgagcg aggacgccgc
     6721 cgccctcgac ttcagcctcg actggcgcgt gctcggcttc gccgccgccg tctcgcttgt
     6781 gagcggcgtc ctcttcggac tcgcgcccgc ccttcaggcg acacggcccg acgtggtcgt
     6841 ggctctcaag gacgacgcgg gcgcgggcgg cgcggggccc cgcaccgtca acctgcgcaa
     6901 cgcgctcgtc gtcgcgcaag tcgccctctc actcgtgctg ctcatcggcg cgggcctctt
     6961 cctgcgcagc ctgcgtaacg ccgcgagcgc cgaccccgga ttcgacgcct cgggcgtgct
     7021 catcgcgcgg ctcgaactcc gcggcgacga ggcgaaggcc gagcgtggac aactcttcta
     7081 cagggaattg caggaacgcg tctcgtcgct cgcgggcgtc cgctcggtgg gcctgacgag
     7141 gatcgtgccg ctcagcggcg g
//
LOCUS       DASVES010000431        11524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1246270, whole genome shotgun sequence.
ACCESSION   DASVES010000431 DASVES010000000
VERSION     DASVES010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246270"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VF240_17650"
     CDS             <1..616
                     /locus_tag="VF240_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177087.1"
                     /translation="AGAFASIVALVALAAIFGSIYFGGGESRQPVGVAQNTPAASAPS
                     SVAPSVTPSVVENPPAPVVTNDKTMTVATNDVKPSKRNRTAAVNNIVRVPKDAREDVA
                     PAGEVAVVQQSVPGEENYLRTIASLSKVVELGGPDVLRPQVRASYERNIVVIDKAIVE
                     TRRAALRNPKDADTTSFLFSAYQNKIDLLSTVADQAQVATLGRD"
     gene            640..1641
                     /locus_tag="VF240_17655"
     CDS             640..1641
                     /locus_tag="VF240_17655"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7177088.1"
                     /translation="MKLLSSILALLVLSAPAVAHGQAQQAPAPPAPPAVSQAERTVAA
                     ARDVLVELSLESGDVVVRGWDRDEVRARSDEAPRIELRKYASPAPGEAKDMSGAAKDA
                     PARRVEVFVLHSKDDPVRPGESSGSGNVELNVPRGATVFLKVQSGDIEVSNVAEARIE
                     SASGDIDLTGISKGVDIDAFSGDISVADSRGSIRLRSLSGSVEATNLKPNDEKDEFTV
                     KSMSGDILLENVTHAKIEGETTSGNVRFDGALATGGGYKLQTTSGDLTMSLPAAASFR
                     VNARIILSGEIITDFVVKTAAPKDKPGPLNRLDGVVGTGDADINLSAFSGTVHLRKN"
     gene            1938..2819
                     /locus_tag="VF240_17660"
     CDS             1938..2819
                     /locus_tag="VF240_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7177089.1"
                     /translation="MNRIAGLLLATALTTQLAAASISGAASSDGGSVASASVAPAVAV
                     VAARPAPQDQGNEFRWTGRLASGRVIEVKGVNGRVNAEGTAAGGEVEVVATKRARQSD
                     PASVRVEVVEHADGVTICAVYPNGDRNKQNRCVPGEGGNMSVQNNDVQVDFTVRVPAG
                     VRFHGKTVNGGVEADNIAADVEAYTVNGGISVSTTGLVKAMTVNGSIRATMGRADWQG
                     ELQFATVNGGIDLIFPSTLSTEVEAETLNGHIETDFPITAQGRLSKRRLEGVINGGGR
                     ELRLKTVNGNVEIRRAS"
     gene            3001..5160
                     /locus_tag="VF240_17665"
     CDS             3001..5160
                     /locus_tag="VF240_17665"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7177090.1"
                     /translation="MMSMTAAALALCLTAGGVRSQQQHTRGDLTEEFHQTYQLAPGGR
                     VHLENINGDVRVRGWDRPEVKVDAVKYAHTRERLEEARIEVDVTPQSVHIETRYPYRS
                     TNWTSDHEGRRNNPATVEYVLTVPRGALISQIELINGALDMEGLTGDVRASSINGKVT
                     ARDLTGPVRLSTINGPLEATFASFTNPISLGSVNGPVSIFLPSDADAEVRAGTVHGAI
                     TNEFNLPVKRGRYVGHNLAGRFGAGTMRVKLDNVNGPIRLRRAQDNRPLGPVTNLLSE
                     TVGGEGEWDGVDREIDEGVSEAAREAQREVREALRESKRERDQERREREREKQQEQRE
                     REREKLGAQRDHLQEERERKQEELERQRERVQEQSERERERLEEQREREREKLEVQRE
                     RQEALRETEQERREALREAEEERRRLRTEATQVAREAARVSREVSREVARAMGGRDRD
                     NSGDQRQIERVSNTLAAVGVPRVRVENFDGPITVIAWDKPEVMYTAVKRAHDQRELQG
                     ISIKTQTDNTTNVNQGTTARADSTVTISTSFDKSKAHQIEAKGAKIDWYSSDASVELE
                     VYVPRKSVLSISSADGRLRVDGVNGEITMHTGDGGIDVSEARGRLRADTNDGRINIVN
                     FNGDVTAQTGDGRILLEGDFGALSARTGDGSISLVVPEGASAIIETNAETVVSDGMAV
                     EEGAGAGGGSLRRWKVGAGGRVYTLRTGDGQIILRRR"
     gene            5405..8572
                     /locus_tag="VF240_17670"
     CDS             5405..8572
                     /locus_tag="VF240_17670"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX7177091.1"
                     /translation="MKNGFSLAAALCVGAAICVLGNPAAAQTPAARALAASRIVADDY
                     APSGTAAASALPVRRIASLRAAETLVGSRVTITFDTPITDYTAARDGGRFLLRVPRAA
                     ASFVPTALVGSGFTEVSVERQRDDLLFTFTLEPATGASVRQSFNRLEVNFVRQAAAQG
                     QQQGSAAQPAPTPAPAGGNPAPASGQTQPADSIPTTTSEEVKATEGKSATALATDVKT
                     PNAGRVNLPPEKAVAVAIRRFDKPPVIDGKLDDEVWKRATIFKDFIQYRPVDLVAPSK
                     PTEAFIGYDSKFIYVAFHAFDEPGKVRATIAKRDAIFDDDTVGIFLDTFDDGRRAYEL
                     FFNPLGVQADATFVEGVSEDFSTDIVMESKGALSDDGYVVEVAIPFKSLRYVAGKDKK
                     WGLHLLRTIKRFNNEQSSWMPISKDNSSILGQRGHLTGLEGVSTERTLELIPSLTVSQ
                     EGQRVRTVSPLDAGRLAGGSDTGRFLNRPAGFDPGLSAKYTITPQVTLDFTYNPDFAQ
                     VEADATVVTANQRFPIFFQEKRPFFLEGKEIFETLISAVHTRAIVDPDYAVKLTGRQG
                     RNTFGVLYASDKAPGNLNEEQRDFVRDTRNPFEEREAFAKILDKNATVGILRFKRDFG
                     QENHIGLLATTYNFIDQYNHVAGADARFRLNKTTTFTTQVLGSVTHRPFFYAEEGGVF
                     DRKEKGMAYSAFINMNGRNWGYEAGITGRSKFFRADVGFNRRFDTNNPVVFVRYQASE
                     RPKNWLVSWRVFNGFNSNFDWKGRHQRYNNETQLQLRLQRQTWLGVGVEEGYERVFEE
                     EFGPTRAAQRLVVERRFSSVLAAGLPACDQFGTLPPPVADDPETPENEELRVPFCTFY
                     GADTERSVNRKSLYAYAESAPSKKYSFNLFTSYNWGVIDLDFGTSNPSYPRVSPAALT
                     LGGDAPLDPGRGNEWFLEMGGAYQPTDALRVSLNYTKNRLTRNDTGLVAFDDNIFSLR
                     GTYQFTRFWFARARADYTTLGSRMRMQYLVGWAPNPGTNFYVGYNDDLNRNGLNPFTS
                     DLEPGFRRNGRLFFIKMSYLFRRSF"
     gene            8942..10141
                     /locus_tag="VF240_17675"
     CDS             8942..10141
                     /locus_tag="VF240_17675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7177092.1"
                     /translation="MAALCALVLAAVSGCKSDYPAGAQQERPGGGGGGAREARQVKTA
                     RVAEMPVGQTVTVNGTLAAYDQAEIGAKVAGRLQIVTVDLGSVVRRGQLVARLDPRDY
                     ELRVQQSVAALQQTRARLGLPLEGNTDRVDPEQTGTVKSARALLEEAKVTRDRAATLV
                     QQGIIARAEYDSASASLRVAESRYQDAIEEIRNREGLLSQRRSELALARQQLTDTAVY
                     APFDGTVEEKLGSVGEFVAAGAPLIRLVKMNPLRFRAEIPEREAASVHAGQNLRVTVE
                     GSPRAYAGRVVRLSPTIKEQNRILVVEADISNDGLLRPGSFARAEIVTSDADMAVTVP
                     TSAVVTFAGIEKVIIVDNGKALEKPITTGRRVSEWTEVVSGVNVGDAVVLEPGNLQTG
                     QPVSVVE"
     gene            10224..>11524
                     /locus_tag="VF240_17680"
     CDS             10224..>11524
                     /locus_tag="VF240_17680"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7177093.1"
                     /translation="MQKLAEICIRRPVFASMIILALVVVGAAGYMGLGVDRFPSVDLP
                     TVSVRTGLPGGSPEEVENLLTRPIEEVINTVDGIDELRSISGQGSSFVIATFKLDRDI
                     ETAAQDVRDRVATVVRQLPEDATPPTVFKFNNDSAPILTVALTADRPLRELTELGDKT
                     VRPQLERVGGVGEAAVVGGIDRAISVWIDAERLAAYQIPITAVRQAISRQNADVPGGN
                     VSSGREELTLRTMGRYTDARAMNDLVVATVDGAPIRLRDVGRVEDGTREQRSLARLNG
                     TPTVTLEIRRQSGANTLDVINGVKRELVRAQAQLPTDVKLEIIRDQSRYIEAAQHEIQ
                     THLILGSILASLVVLLFMRSWRSTLIAAVAIPASVISTFGMMRALDFTLNSVTMLALV
                     LMVGVVIDDAIVVLENIFRFIEEKKMHPAEAAREATKDIGLA"
BASE COUNT         2115 a         3967 c         3690 g         1752 t
ORIGIN      
        1 ggccggcgcg tttgcgagca tcgtcgccct cgtcgccctc gcggcaatct tcgggagcat
       61 ctacttcggc ggcggcgaga gccggcagcc cgtgggcgtg gcccaaaaca ctcccgcggc
      121 ctccgcgccg tcctctgtgg ccccaagtgt gactccgtca gtcgtcgaaa atcctccggc
      181 gccggttgtc acgaatgata agacgatgac ggtggccacg aacgatgtaa agccgtcaaa
      241 gcgcaacagg acggccgctg tgaacaacat tgttcgtgtg ccgaaagatg cgagggaaga
      301 tgtcgcgccc gccggtgagg tcgctgttgt gcagcagtca gtgcccggcg aagagaacta
      361 tctgaggacc atcgcgtcac tctcgaaggt ggtcgaactc ggcggccccg acgtgctccg
      421 gccgcaggtg cgcgcctcct atgagcgcaa catcgtcgtc atcgacaagg ccatcgtgga
      481 gacgcgccgc gcggcgctcc gcaacccgaa agacgccgac accacctcgt tcctgttctc
      541 ggcctaccag aacaagatcg acctgctctc gaccgtcgcc gatcaggcgc aggtcgcgac
      601 cctcggacgt gattaaattt gagacggact tatcctgaca tgaaactgct ttcatccatt
      661 ctcgctctac tcgtcctctc ggcgcccgct gtcgcgcacg ggcaggcgca gcaggcgccc
      721 gccccgccgg ccccgccggc cgtgtcgcag gccgagcgca cggtcgccgc cgcgcgagac
      781 gtactggtcg agctgtcgct cgaatccggc gacgtggtcg tgcgcggctg ggatcgcgac
      841 gaggtgcgcg cgcgctccga cgaggccccg cgcatcgagc tgcgtaaata tgcgtcgccc
      901 gcccccggcg aggcgaagga catgtcgggt gcggccaaag acgcgccggc gcgtcgcgta
      961 gaggtcttcg ttcttcactc gaaggacgac cctgtgcggc cgggcgagtc gagcggttcg
     1021 ggcaacgtcg agctgaacgt cccgcgcggc gcgaccgtct tcctcaaggt gcagagcggc
     1081 gacatcgaag tctccaacgt ggccgaggcc cgcatcgaga gcgcctccgg cgacatcgac
     1141 ctcacgggca tctcgaaggg cgtcgatatc gacgcgttca gcggcgacat ctcggtcgcc
     1201 gactcgcgcg gttcgattcg tctccgctcg ctgagcggct cggtcgaggc gacgaacctg
     1261 aagccgaacg acgaaaagga cgagttcacc gtcaagtcga tgagcggcga catccttctg
     1321 gagaacgtca cacacgcgaa gatcgagggc gagaccacga gcgggaacgt gcgcttcgac
     1381 ggcgcgctcg ccacgggcgg cggctacaag cttcagacga cgagcggcga cctgacgatg
     1441 tctctgcccg ccgccgcttc cttccgcgtt aacgcccgta tcatcctcag cggcgagatc
     1501 atcaccgact tcgtcgtcaa gaccgcggcc ccgaaggaca agcccggccc gctcaaccgg
     1561 ctcgacggcg tcgtcggcac aggcgacgct gatatcaatc tctcggcctt cagcggcacc
     1621 gtccatctca gaaagaattg acgctccgca acgcgcggtt ttcgcgcgcg cccgtccagg
     1681 agcacgacga gggatgaggg cggagggatg aaggatgaag gggagaccag tcctgtctga
     1741 ttcatcccgc atgcctccgc cctcatccct tgcctccccc gctcgcggta ctgatttttc
     1801 tgccacaagt gccgcgcgcg cggtgttcaa acagtagata ggcagctacc gggtagccga
     1861 aacgtgcggt ggggctgcgg tcgaagaatt ctatgaccgg cgtcgggcgt cgggaatcga
     1921 ggagaaagag aatgcacatg aacaggatcg ccggccttct tctggcaaca gctttgacga
     1981 cgcagctcgc ggccgcgtcc atttccgggg ccgcgtcgag cgacggcggg tcagtcgctt
     2041 ccgcgtcggt cgcgccggcc gtcgccgtgg tcgccgcccg gcccgcgccg caggatcagg
     2101 gcaacgagtt tcgctggacg ggccggctcg cttcggggcg tgtgattgaa gtgaagggcg
     2161 tgaacgggcg cgtcaacgcc gaggggacgg cggccggcgg cgaggtggaa gtggtggcca
     2221 cgaagcgcgc gcgccagagc gacccggcga gcgtgcgcgt cgaggtggtg gagcacgccg
     2281 acggcgtgac catctgcgcc gtctacccga acggcgaccg gaataagcag aaccgctgcg
     2341 tccccggcga gggcgggaac atgagcgtcc agaataacga cgtgcaggtc gatttcacgg
     2401 tgcgcgtgcc ggcgggcgtg cgcttccacg gcaagaccgt caacggcggc gtcgaagccg
     2461 acaacatcgc ggccgacgtg gaagcctaca cggtcaacgg cggcatcagc gtctcgacca
     2521 cgggacttgt taaggccatg accgtcaacg gctccatcag ggccacgatg gggcgcgccg
     2581 actggcaggg cgagcttcag ttcgccaccg tcaacggcgg catcgatctc atcttcccct
     2641 cgacgctcag caccgaggtg gaagccgaga cgctcaacgg ccacatcgag accgacttcc
     2701 ccatcaccgc gcagggccgt ttaagcaagc gccgactcga aggcgtcatc aacggcggcg
     2761 ggcgcgagct gcgcctcaag acggttaacg gcaacgtcga gattcgccgc gcgtcgtgag
     2821 caagctatta gctgttagct attagctatc agcaagaggc aggcgggcga aggattttat
     2881 ttcatcggcg gcagcggatt aggcggcaac gggccgtgcg ctgccggcaa acagctaaca
     2941 gctaatcgct aatagctaac taatactgga aggtgatccg agatgatgag taagcgtcga
     3001 atgatgagca tgaccgcggc ggccctggcg ctgtgtctga cggcgggcgg cgtgcgctcg
     3061 cagcagcagc atacgcgcgg cgacctgacg gaagagttcc accagaccta tcagctcgcg
     3121 ccgggcgggc gcgtccatct ggagaacatc aatggcgacg tgcgcgtccg cgggtgggat
     3181 cgccccgagg tcaaggtcga tgccgtcaag tacgcccaca cgcgcgagcg tctggaggag
     3241 gcccgcatcg aggtggacgt taccccgcag tccgtccaca tcgagacgcg ctacccgtat
     3301 cgctcaacga actggacgag cgaccacgag ggccgacgca acaacccggc cacggtcgag
     3361 tatgtgctca cggttccgcg cggggcgctc atcagccaga tcgaactcat caacggggcg
     3421 ctcgacatgg agggtctgac cggcgacgtt cgggcctctt cgatcaacgg caaggtcacg
     3481 gcgcgcgacc tgaccggccc cgtccgcctc tcgaccatca acggcccact ggaggcgacc
     3541 ttcgcgagct tcaccaatcc gatctcgctc ggctccgtca acgggccggt cagcatcttc
     3601 ctcccctccg acgccgacgc cgaagtgcgc gccggcaccg tccacggcgc gatcaccaac
     3661 gaattcaatc tacccgtgaa gcgcggccgc tacgtcggcc acaacctcgc cggacgtttc
     3721 ggcgccggga ccatgcgcgt caagttggac aacgtcaacg gccccatccg cctccgccgc
     3781 gcgcaggaca atcgccccct cggccccgtg accaatctcc tctcggagac ggtcgggggc
     3841 gaaggcgagt gggacggcgt tgaccgggag atcgatgagg gcgtgagcga ggccgcgcgc
     3901 gaggcgcagc gcgaggtgcg cgaggctctg cgggagtcga agcgcgagcg cgatcaggag
     3961 cggcgtgagc gcgagcgaga gaaacagcag gagcagcgcg agcgcgagcg cgagaaactc
     4021 ggcgcgcagc gcgaccatct ccaggaggag cgcgagagga agcaggagga gttggagcgg
     4081 cagcgcgagc gtgtgcagga acagagcgaa cgtgagcgcg agcgtctgga agagcagcgc
     4141 gagcgggaac gcgagaagct ggaagtgcag agggagcggc aggaagcttt gcgcgagacg
     4201 gagcaggagc gccgcgaggc gttgcgcgag gccgaggagg agcgccgccg cctgcgcacc
     4261 gaggccacgc aggtcgcccg cgaagccgcg cgcgtctccc gagaggtgtc gcgcgaggtc
     4321 gcgcgcgcca tggggggtcg cgaccgtgat aacagcggcg accagcgaca gatcgagcgc
     4381 gtgtcgaaca cgctggcggc cgtcggcgtg ccgcgcgtga gggtcgagaa tttcgacggc
     4441 cccatcaccg tcatcgcgtg ggacaagccg gaggtgatgt acacggccgt caagcgcgcc
     4501 cacgaccagc gcgagttgca gggcatctcc atcaagaccc agaccgacaa caccacgaac
     4561 gtcaatcagg gaaccaccgc gcgcgccgac tccacggtca ccatctccac cagcttcgac
     4621 aagtcgaagg cgcaccagat cgaggccaag ggggcgaaga tcgactggta cagctccgac
     4681 gcctccgtgg agcttgaggt ctacgtgccg cgcaagtccg tcctcagcat ctcctcggcc
     4741 gacgggcggc tgcgcgttga cggcgtcaac ggtgagatca ccatgcacac cggcgacggc
     4801 ggcatcgacg tgagcgaggc ccgtggtcgc ctgcgtgccg acaccaacga cggccgcatc
     4861 aacatcgtca acttcaacgg cgatgtgacc gcgcagaccg gcgacggccg catcctcctc
     4921 gaaggcgact tcggcgcgct ctcggcgcgc accggcgacg gctccatctc gctcgtcgtg
     4981 cccgaaggcg cgagcgccat catcgagacc aacgccgaga cggtcgtcag cgacgggatg
     5041 gccgtggagg aaggagcggg cgcgggcggc ggaagcctgc gccgctggaa agtgggcgcg
     5101 ggcgggcgcg tctacacgct ccgcacgggc gacggccaga taattctgcg gcggcgctga
     5161 caacctgcgc cgcctctgcg cgcctccaag cgccagaggc gggcggccat gtcggaagtc
     5221 ggaggaaagg aacattctcc gcttcgcgaa cgtctaagtg tggcgaagcg cgaactcgac
     5281 aggtcgcccg gctaatcaat cggatttatt tctcgaacat cggacacaag ctcgtgagcg
     5341 tttgacgagc cgggcgcttc gtacacccgc gacgaagcgc cgcatcacaa atggaatcct
     5401 cgaaatgaag aatggcttta gtctggccgc cgccctctgc gtgggcgcgg ccatttgtgt
     5461 tttaggtaac ccggccgcgg cccagacgcc cgccgcgcgc gccctcgccg cctcgcgcat
     5521 tgtcgccgac gactatgcgc cgtcgggcac ggccgccgcg tcggcgcttc ccgtcaggcg
     5581 catcgcctct ctgcgcgccg ccgagacgct cgttggctcg cgcgtgacca tcacgttcga
     5641 cacgcccatc accgactaca cggccgcgcg cgacggcggg cggtttctgc tgcgcgtccc
     5701 gcgcgccgcg gcgtcgttcg tcccgaccgc gctcgtcggc agcggcttca ccgaggtctc
     5761 ggtcgagcgg caacgggacg atctgctctt cacgttcact ttggagccgg cgacgggcgc
     5821 gagcgtccgc cagagcttca accgcctcga agtgaacttc gtccggcagg cggcggcgca
     5881 ggggcagcag caaggctcgg ccgcgcagcc cgccccgacc cccgcgcccg cgggcggcaa
     5941 cccggccccg gcttcgggtc agacgcagcc cgccgacagc atccccacca ccacctccga
     6001 ggaggtcaag gccaccgaag gcaagtcggc gacggcgctc gccactgatg ttaagacccc
     6061 gaacgcgggg cgcgtcaacc tcccgccgga gaaggccgtc gccgtcgcca tcaggcgctt
     6121 cgacaagccg cccgtcatcg acggcaagct cgacgacgaa gtgtggaagc gggcgacaat
     6181 cttcaaggac ttcatccagt accggcccgt cgatctggtc gcgccctcga agccgaccga
     6241 ggccttcatc ggctacgact cgaagtttat ctacgtcgcc ttccacgcct tcgacgagcc
     6301 gggcaaggtg cgcgccacca tcgccaaacg cgacgccatc ttcgacgacg atacggtcgg
     6361 catcttcctc gacaccttcg acgacggccg gcgcgcctac gagctgttct tcaacccgtt
     6421 gggcgtgcag gccgacgcca cgttcgtcga gggcgtgagc gaagacttca gcacggacat
     6481 cgtcatggag tcgaagggcg cgctcagcga cgacggctac gtcgtcgagg tcgccatccc
     6541 gttcaagtct ttgcgctacg tcgccggcaa ggacaagaag tggggcttgc acctgctgcg
     6601 caccatcaag cgtttcaaca acgagcagag ttcgtggatg ccgatctcga aagacaactc
     6661 cagcatcctc ggccagagag gacacctcac cggcctcgaa ggcgtctcca ccgagcgcac
     6721 cctcgaactc atcccgtcgc tgaccgtctc gcaggagggg cagcgcgtcc gcaccgtcag
     6781 cccgctcgac gccggccgcc tcgccggcgg ctccgacacg ggccgcttcc tcaaccgccc
     6841 cgccggcttc gatcccggtc tgtcggcgaa gtacaccatc acgccgcagg tcacgctcga
     6901 cttcacttac aatcccgact tcgcgcaggt cgaggccgac gccacggtgg tcacggccaa
     6961 tcagcgcttc cccatcttct ttcaggagaa gcgccccttc ttcctcgaag gcaaggagat
     7021 tttcgagacg ctcatcagcg ccgtccacac gcgcgccatc gtcgatcccg actacgccgt
     7081 caagctcacg ggccggcagg gacgcaacac cttcggcgtg ctctacgcct ccgacaaagc
     7141 gcccggcaac ctcaacgaag aacagcgcga cttcgtccgc gacacccgca acccatttga
     7201 ggagcgcgag gcgttcgcca agattctcga caagaacgcg accgtcggca tccttcgctt
     7261 caagcgcgac ttcgggcagg agaaccacat cgggctgctg gcgacgacct acaacttcat
     7321 cgaccagtac aatcacgtcg ccggcgccga cgcccgcttc cgtctcaaca agacgacgac
     7381 cttcaccacg caggtcttgg gcagcgtcac gcaccgcccc ttcttctacg cggaggaggg
     7441 gggcgtcttc gaccgcaaag agaagggaat ggcctacagc gccttcatca acatgaacgg
     7501 ccgcaactgg ggctacgagg ccggcataac ggggcgctcg aagttcttcc gcgccgacgt
     7561 gggcttcaac cgccgcttcg acaccaacaa ccccgtcgtc ttcgtccgct atcaggcgag
     7621 cgagcgcccg aagaactggc tcgtcagttg gcgcgtcttc aacggcttca actcgaactt
     7681 cgactggaag ggacgccacc agcgttacaa caacgagacg cagctccagc tccgccttca
     7741 gcggcagacg tggctgggcg tcggcgtcga ggagggctac gagcgcgtct tcgaggagga
     7801 gttcggcccc acgcgcgcgg cgcaacgcct cgtcgtcgag cgccggttca gctccgtact
     7861 ggcggccggt ctccccgcgt gcgaccagtt cggcacgctc ccgccgcccg tcgccgacga
     7921 ccccgagacc ccggagaacg aggagctacg cgtccccttc tgcaccttct acggcgcaga
     7981 cacggagcgc tccgtcaatc gcaagtcgct ttacgcctac gccgaatcgg cgccgtcgaa
     8041 gaagtattcc ttcaacctct tcacctccta caactggggc gtcatcgacc tcgacttcgg
     8101 aacctccaat ccgagctacc cgcgcgtcag tcccgcggcg ctcaccctcg gcggcgacgc
     8161 gccgctcgat cccggccgtg gcaacgagtg gttcctcgaa atgggcggag cctaccagcc
     8221 gaccgatgcg ctgcgggtct cgctcaacta cacgaaaaac cggctcacgc gcaacgacac
     8281 cggcctcgtc gccttcgacg acaacatctt ttccctgcgc ggcacctatc agttcacccg
     8341 cttctggttc gcccgcgcgc gcgccgacta cacgacgctc ggctcgcgca tgcggatgca
     8401 ataccttgtg ggctgggcgc cgaaccccgg cacgaacttc tacgtcggct acaacgacga
     8461 cctcaatcgc aacggcctga accccttcac gagcgacctc gaacccggct tccgccgcaa
     8521 cggccgactc ttcttcatca agatgtccta cctgttccgg cgcagttttt aagtgcgcgc
     8581 gcgtcgcccc cgagcgcgcg cggcgggcgc attgagcgac aacagtaccg cgagcggtag
     8641 cgagcgggtg cggcacacgt gaggcgagcc tccagccaca gcgtcacccg cgaggcttcc
     8701 gctcgcggta ctgtttagac gcatggccac gacacttcat acgcatggct acgacacctc
     8761 ttttttacat tcttttgaca cttcatcacg cccgcaggca aaaccccgct caggtttcaa
     8821 atctaagcgt gttagacttt agtacccccg agccgaagcg ccgccgcgac cgtcaattca
     8881 gtcgaacatt tgtatgcagt catcaagggc ttattcattt tcccggaacg gccaacggac
     8941 tttggccgca ttgtgcgcgc tcgtgctcgc tgcggtgagc ggttgcaaga gcgattatcc
     9001 ggcgggcgcg caacaagagc ggccgggcgg cggtggcggt ggggcgcgcg aggcgcggca
     9061 ggtgaagacg gcgcgcgtcg ccgagatgcc cgtcggacag accgtcacgg tcaacggcac
     9121 actcgccgca tacgatcagg cggaaatcgg cgcgaaggtt gccggccggt tgcaaatcgt
     9181 cacggtcgat ctcgggagcg tcgtccgccg cggtcagctc gtcgcgcggc tcgacccgcg
     9241 cgactacgag ttgcgcgtcc agcagtccgt ggccgcgctt cagcagacgc gcgcccgtct
     9301 ggggttgccg ctcgaaggca ataccgaccg cgtcgatccc gagcagacgg gcacggtcaa
     9361 gtcggcgcgc gccctgctcg aagaggcgaa ggtgacgcgc gaccgcgccg ccacgctcgt
     9421 ccagcaggga attatcgccc gcgccgagta cgactcggcc agcgcctctc tgcgcgtggc
     9481 cgagagtcgc tatcaggacg ccatcgaaga gatacgcaac cgcgaagggc tgctctcgca
     9541 acggcgctcg gagctggcgc tcgcccggca acaacttacc gacacggctg tctacgcgcc
     9601 tttcgacggg acggtcgagg agaagctggg gagcgtgggc gagttcgtcg cggccggcgc
     9661 gccgctcatc cgtctggtga agatgaaccc gctcaggttc cgcgccgaga ttcccgaacg
     9721 cgaggcggca agcgtccacg ccgggcagaa tttgcgcgtc acggtcgaag gctcgccgcg
     9781 cgcctacgcg ggccgggtcg tccgcctcag cccgaccatc aaggaacaga accgcatcct
     9841 cgtcgtcgag gccgacatct ccaacgatgg cctgctgcgc cccggctcct tcgcccgcgc
     9901 cgagatcgtc acgagcgatg ccgacatggc cgtcaccgtc ccgacgagcg ccgtcgtcac
     9961 cttcgccggc atcgagaagg tcatcatcgt ggacaacggc aaggctctgg agaagccgat
    10021 cacgacgggc cgccgcgtct ccgaatggac ggaggtcgtc agcggcgtca acgtcggcga
    10081 cgcggtcgtc ctcgaacccg gcaacttgca gacggggcag cccgtcagcg tggtcgagta
    10141 aagttcaaag ttccgagttc aaagttcaaa ggccgaacag cttttgactt tgaactttga
    10201 actttgaact cggaacttga gaaatgcaaa agctcgcgga aatttgtatc aggcgtccgg
    10261 tcttcgcgtc gatgatcatc ctcgcgctgg tcgtggtcgg cgcggccggg tacatgggcc
    10321 tcggcgttga ccgcttcccg tcggtcgatc tgccgacggt gagcgtccgc acgggtctgc
    10381 ccggcggctc gcccgaggaa gtcgagaacc tcctcacgcg gcccatcgag gaggtcatca
    10441 acacggtgga cggcatcgac gagctgcgct cgatttccgg gcaaggctcc tcgttcgtca
    10501 tcgccacctt caagctcgac cgcgacatcg agaccgccgc gcaggacgtg cgcgaccgcg
    10561 tcgccacggt cgtccgccaa ctccccgagg acgccacgcc ccccaccgtc ttcaagttca
    10621 ataacgacag cgcgccgatt ttgaccgtcg cgctcacggc cgaccggccc ctgcgcgagc
    10681 tcaccgagct gggcgacaag accgtgcgcc cgcagctcga gcgcgtcggc ggggtgggcg
    10741 aggcggccgt tgtcggcggc atcgaccgcg ccatcagcgt ctggatcgac gccgagcgcc
    10801 tcgccgctta ccagattccg attaccgccg tccggcaggc catctcgcgg cagaacgcgg
    10861 acgtgccggg cggcaacgtc tcatccggcc gcgaggaatt gacgctcagg acgatggggc
    10921 gctacaccga tgcgcgcgcg atgaacgacc tcgtcgtcgc taccgtggac ggcgcgccca
    10981 tccgcctgcg cgacgtgggc cgcgtcgagg acggcacgcg cgagcagcgc agcctcgcgc
    11041 gcctcaacgg cacgcccacc gtcacacttg aaatccgccg ccagtcgggc gccaacacgc
    11101 tcgacgtaat caacggcgtc aagcgcgagc tggtgcgggc gcaggcgcaa ctgccgacgg
    11161 acgtgaagct ggaaatcatt cgcgaccagt cgcgctacat cgaggcggcg cagcacgaga
    11221 ttcagacgca cctcattctg ggcagcatcc tcgcctcgct cgtcgttctg ctcttcatgc
    11281 gctcatggcg ctcgacgctg attgccgccg tcgccatccc ggcctcggtc atctccactt
    11341 tcggcatgat gcgggcgctc gacttcaccc tcaactccgt gacgatgctc gcgctcgtcc
    11401 tcatggtcgg cgtggtcatc gacgacgcca tcgttgtgct cgaaaatatc ttccgcttca
    11461 tcgaggagaa gaagatgcac cccgcggagg cggcgcgcga ggcgaccaaa gatatcggtc
    11521 tcgc
//
LOCUS       DASVES010000432         9766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_822649, whole genome shotgun sequence.
ACCESSION   DASVES010000432 DASVES010000000
VERSION     DASVES010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822649"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..2850)
                     /locus_tag="VF240_17685"
     CDS             complement(70..2850)
                     /locus_tag="VF240_17685"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HEX7177094.1"
                     /translation="MNGRMRVSRLAAGALALAGLVGALVPPGASVASAQGQNQEKSAS
                     QEQEQERRGRIAFASDREDNFEIYTVEEDGGGLLRLTTDPAEDTDPSWSPDGKRIAFT
                     SDRDGNQNIYVIDATTMAETRLTDNAANDLDPAWSPDGSKIAFTSERNGHQDVYVMNA
                     DGTAQTRLTDSTADDFRPSWAPSGALLAFTSNRDGNDEIYRMDANGANQVNLTNNPAE
                     DLNPSWPPGRIVFQSTRDGNDEIYIMNANGTNPQRLTNNPAFDGRPGASPDGERIAFT
                     SDRDGNNFEIYVMTASGASQTRFTGDEANDLDPDIQPKATTSPGSSFQFGATALAVSE
                     GSGRVTLTITRTGSTAGSATVEFATVSGSASERSDFNPAFGRLRFAAGETSKTIDVLI
                     VDDALVESDETFSVTLRNPTGSLLTSAFTATVTIQDNDASAPTILYAVDSANNLLQLD
                     AAAPGAVVSTNAITGLQAGESVVGIDVRPATTQLYALGSSGRLYTVNPSNGQAQLAST
                     VSSALSGTSFGVDFNPVPDRLRVTSEADQNLRINVDNGAATVDGTLAYAAGDTNAGQD
                     PNVVGSAYTNSFAGATVTTLYGIDSTRDTLVTQNPPNAGTLNTVGPLGVDTGNLVGFD
                     IATVGNAAFAALNTGAASRLYRINLSTGAATLVGDIGGGSVIRALAVANQPANPIDET
                     GFFVRQQYLDFLNREPDAEGFAFWTGIINSCGGDQLCRQARRADVSGAFFLSIEFQGT
                     GYFVYRAYQAAYGRQPRFAEFLPDTQRISQGVVIGQPGALERMEANRREYADEFVIEQ
                     EAFRTLYDGLTNEQYVDALNVNTGGSLSAAERNALVAGLGSGTETRATVLIKVADDAD
                     FRAREFNRAFVLMQYFGYLRRNPDDPPDTDFSGYNFWLAKLNQFGGDFRAAEMVKAFI
                     TSGEYRRRFGPA"
     gene            3335..4825
                     /locus_tag="VF240_17690"
     CDS             3335..4825
                     /locus_tag="VF240_17690"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX7177095.1"
                     /translation="MRKILTAVAAGALALAMLSGGPIRPTQASSHREAPLIVADPLAD
                     NTDVYAFRSTEAGREGFVTLIANYVPFQEPSGGPHFYPFDDNVLYEIKIDNTGDGNAD
                     VTYQIRFRTEVRNPDIVLGQAAPNQALAGTGGIEPLITSLDDPDYNEPQFYTITRVNR
                     ASRTSTVLGTNLPTPPANVGERTTPNYETALGAPAVRNLSNGGRAFAGQRDEGFYIDV
                     GAIFDTLNLRSITDTGGVDSTSGFNVNTIAIEVPIQELTRTGTVPASSTADGAVVGVW
                     ATASRRSTTVLRPGKRVVTGPFRQVSRLGNPLVNEVIIPLKLKDAFNSLNPSGDAVAA
                     PFVLDPQLAQLLKAVFGINVPAAPRNDLVSIFATGIPVNAITGPTYTTFLSDGTPHEM
                     MRLNVAIAPAASPNRLGLLGNDVAGFPNGRRVGDDVVDIALRAVAGGTPFTPETNSPP
                     NSTLGDGVDRNDVPYLMRFPYLGTPHSGNTKMGKPHEHHNVIPSSE"
     gene            4934..6253
                     /locus_tag="VF240_17695"
     CDS             4934..6253
                     /locus_tag="VF240_17695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177096.1"
                     /translation="MKTSKLVMLVSLGAVVLAAGALGFRQYSQNSAGRAAASASGESS
                     AQPASSEKQTPADVRIRAGEGKIKIAPDKPDGYNLLASAYMQKARETGDYGVNAKAVE
                     ALEQSFRVAPDNYDAIKLNAKVLLTYHRFEEGLAEAERARKQNPEDHDVYGALVDANV
                     ELGRYDAAVDAAQRMVDLRPDAAAYSRIAYMRELHGDTEGAVEAMSVALKATDPRDPE
                     AVAWARVQLGHALASVGKRAEAEREYDHALRVLPGYGAALTSKADARAAAGDFDAAVE
                     IYKQFDSHDAHLALGDLYARLGRTEDAAREYESFERAEHEAAAEENDMSHLTRFWADQ
                     GKNLDEAADIMRRERVRRGDIYTCDTLAWVLYKKGDLAAARQAIDEALRLGTRDAKIN
                     YHAGMIYQALGESRRAARHLKLALDINPSFDVLQAEAARRALDSLNV"
     gene            complement(6482..6616)
                     /locus_tag="VF240_17700"
     CDS             complement(6482..6616)
                     /locus_tag="VF240_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177097.1"
                     /translation="MQAIIPRGRRKHPRAKALGWMALMPFRSRRGRRPTRRKSSDLRR
                     "
     gene            6764..9313
                     /gene="topA"
                     /locus_tag="VF240_17705"
     CDS             6764..9313
                     /gene="topA"
                     /locus_tag="VF240_17705"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX7177098.1"
                     /translation="MAKNLVIVESPAKAKTINKYLGSDYKVMASYGHVKDLPTKGLGV
                     DVENNFQPTYEVIPETRKRNNKKTLAELKKAAKEAEAIYLAADPDREGEAICQHLAEE
                     IVPKRPAKPHFRVMFNEITKRAVQAAFDHPMQIDQHLVDAQQARRVLDRLVGYKVSPL
                     LCRTIGGKLSAGRVQSVALRMVVERERDIEKFIKTEYWTIAANVSANLPPAFDARLYK
                     VGEQTVKTGAFDQDAKKGEIHIKDEATASALVAEAGGEQFVVAEVATKERKRNPVPPF
                     ITSKLQQEASRKLGFAVKKTMMLAQKLYEGVEIGSEGSVGLITYMRTDSTRVSETALG
                     EARDFVGTQYGTQYLPEKAIHYRSKKDAQDAHEAIRPTEVARTPDSVASYLGRDELRL
                     YRLIWQRFVASQMMPAVFDQTTIDISAGRLLFRATGSVLKFDGFLRVYEEGHDEKTEE
                     DDEAGRKLPPVEQGERLRLNAVTPEQHFTEPPPRYTEATLVKALEEKGIGRPSTYAQI
                     MSTIIDREYVEKLEGRFHPTALGMTVNDLLIEGGFADLFNETYTARMEEELDEVEEGK
                     MKWTEALAEFYEKFARDLKEFEKYTKTKKGMSLPTDEVCPVDSAPMVIKFGRFGQFLA
                     CSNYPECRTTREVAKPQAAAGEAGAEGAAEGSVPAAEDESCELCGKPMALKRGRFGQF
                     LGCTGYPECRNIRKISRKGVVAPPPVPIEGETCPVDGAQLVRRHGPYGEFVSCANYPT
                     CKYIKRETTGVACLRPGCRGELLVKKSKRGKYFYGCSEYPKCDAVYWDKPVASPCPQC
                     NAPFVLEKTTKKGTFRICARGEECGYREERLPQAPPETRAGEGVTPPHAAS"
     gene            9531..>9766
                     /locus_tag="VF240_17710"
     CDS             9531..>9766
                     /locus_tag="VF240_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177099.1"
                     /translation="MLRKRIAALLLCTLFLTGLPAQAQQVVVVNAPPQAKADPNDPVE
                     RIKDEGLNRSQVMQTLSYLTDVIGPRLTNSPQMKR"
BASE COUNT         1795 a         3191 c         3221 g         1559 t
ORIGIN      
        1 ccggcggacg ttaatctgtc cgccggccgg ctcgcgtttg accgattgtt tggttcaccg
       61 cccggagcct taagcaggcc cgaagcggcg gcggtattcg ccgctggtga tgaaggcctt
      121 gaccatctcg gccgcgcgga agtcgccgcc aaactggttg agcttcgcga gccagaagtt
      181 atagccggag aagtccgtgt ccggcggatc gtccgggttg cgccggagat agccgaagta
      241 ctgcatcagc acgaacgcgc ggttgaactc ccgcgcgcgg aagtccgcgt cgtcggccac
      301 cttgatgagc acggtcgcgc gcgtctccgt gccggagccc agaccggcga ctaacgcgtt
      361 gcgctcagcg gcggagagtg agccgcccgt gttgacgttt aaggcgtcaa cgtactgctc
      421 gttggtcagc ccgtcgtaga gcgtgcggaa cgcctcctgc tcgatgacga actcgtcggc
      481 gtactcacgc cggttcgcct ccatgcgctc cagcgcgccg ggttggccga tgacgacgcc
      541 ctgactgatg cgctgcgtgt caggcagaaa ctccgcgaag cgcggctgcc ggccgtaggc
      601 ggcttgatag gcgcgataga cgaagtagcc cgtcccctga aactcgatgg agaggaagaa
      661 agcgccggac acgtcggccc tgcgcgcctg acggcacaac tgatccccgc cgcacgagtt
      721 gatgatcccg gtccagaagg cgaagccttc ggcgtccggc tcgcggttga ggaagtcgag
      781 atactgctgg cggacgaaga agcccgtctc gtctattggg ttggccggct ggttagcgac
      841 ggcgagggcg cggattacgg agccgccgcc gatgtcgccg acgagcgtgg ccgcgcccgt
      901 cgagaggttg atgcggtaga gacgcgaggc cgcgcccgtg tttagcgcgg cgaaggccgc
      961 gttgccgacc gtggcgatgt cgaagccgac gaggtttccg gtgtccacgc cgagcgggcc
     1021 gacggtgttg agcgtccccg cgttgggcgg gttctgcgtg accagcgtgt cgcgcgtcga
     1081 gtcgatgccg tagagcgtgg tgacggtcgc gccggcgaag ctgttggtgt aagccgagcc
     1141 gacaacgttc gggtcctgcc cggcgttcgt gtcgccggcc gcgtaggcga gcgtgccgtc
     1201 tacggtggcc gcgccgttgt ccacgttgat gcgaaggttc tgatccgctt cgctcgtcac
     1261 gcgcaggcgg tcgggcacgg ggttgaagtc aacgccgaag ctcgtccccg acagcgccga
     1321 cgagacggtg gaggccagtt gcgcctgacc gttcgacggg ttgacagtgt agaggcggcc
     1381 cgaactgccg agcgcgtaga gctgcgtggt ggccggtcgc acgtcgatgc cgacgacgct
     1441 ctcgcccgcc tggaggccgg tgatggcgtt cgtcgagacg acggcgccgg gagcagcagc
     1501 atcgagctgc aacaggttgt tcgccgagtc aaccgcataa aggatcgtcg gggcggaagc
     1561 gtcgttgtcc tgaatcgtca ccgtcgccgt gaaggccgag gtgaggagcg accccgtagg
     1621 gttgcggagc gtgacgctga acgtctcatc cgactcgacg agcgcgtcgt ccacgatgag
     1681 cacgtcgatg gtcttgctcg tttcgccggc ggcgaagcgt aagcggccga aagcggggtt
     1741 gaagtcggaa cgctcggagg ccgagccgct cacggtggcg aactcgaccg tggccgagcc
     1801 ggccgtgctc ccggtgcgcg tgatggtcag cgtgacccgg cccgaccctt cggaaacggc
     1861 gagcgccgtc gcgccgaact ggaaggagct gcccggcgag gtcgtcgctt tcggctggat
     1921 gtccgggtcg aggtcgttcg cctcgtcgcc cgtgaaccgc gtctgggaag cgccgctcgc
     1981 ggtcatcacg tagatttcaa aattgttgcc gtcgcggtcg ctcgtaaacg cgatgcgctc
     2041 gccgtcgggc gaggctccgg gccgtccgtc gaaggcgggg ttgttggtga gacgctgcgg
     2101 gttcgtcccg ttggcgttca tgatgtaaat ctcgtcgttg ccatcgcgcg tgctctggaa
     2161 gacgatgcgg cccggcggcc acgacgggtt caaatcctcg gccgggttgt tcgtgaggtt
     2221 cacctgattg gcgccattgg cgtccatgcg gtaaatctca tcgttgccgt cgcgattgga
     2281 ggtgaaggcg agcagggcgc cgctgggcgc ccacgagggg cggaagtcgt cggccgtgct
     2341 gtcggtcaga cgcgtctggg ccgtgccgtc ggcattcatc acgtacacgt cctgatggcc
     2401 gttgcgctcg ctggtgaaag cgatcttcga gccgtcgggc gaccacgcgg ggtcaaggtc
     2461 gttcgccgcg ttgtcggtca gacgcgtctc ggccatcgtc gtcgcgtcga tgacatagat
     2521 gttctggttg ccgtcgcggt cgctcgtgaa ggcgatccgc ttcccgtcgg gcgaccaact
     2581 ggggtcggtg tcctcggccg ggtcggtggt cagacgcagc aagccgccgc cgtcttcttc
     2641 caccgtgtag atttcaaaat tgtcctcgcg gtcgctggca aaggcgatgc ggccccgccg
     2701 ctcctgctcc tgctcctgcg acgcggattt ttcctgattc tgcccctggg cagaggcgac
     2761 ggaggcgccc ggcggcacca gagcgccgac cagccccgcg agcgccaacg ccccggcggc
     2821 cagacgactg acacgcattc tcccgttcat atagacctca ttccttgttt cgacccgagc
     2881 tcgctctccg ggtttatcaa tccaatctgg agccacgccg gacgactcaa cattaaggag
     2941 ccgtcaatcg acaggcaggg aaataactga gcaaagacgg gcagcccaag acgcttccgc
     3001 cgccgctgga gttgaccgcc gaccccttct ttcggccgcg ccactttgcg ccgccgaaaa
     3061 aaaatcgggc ctgccgggcc gaccataaat ccaaaaaacc gccgccctcg tagattgtgc
     3121 agatttcaga aaaactgtcc taactacgcg acacggagtc tgaacggatt tagacagact
     3181 cttcaggggt gaaaattatt ttccgaggcg ccgctccggc cgacaccgac ggccgcggcc
     3241 cttcggcgcc tctcgcgcac gactacgcgc gccgccgagg cgcctttcaa accagacggg
     3301 ttggttcaac gaaagtatga aagggaacac agggatgcga aaaatattga cagcagtagc
     3361 cgccggcgcg ctcgcgctgg ccatgctctc gggcggcccc atccgcccga cacaggcttc
     3421 gagccaccgc gaagcgccgc tcatcgtggc cgacccgctg gccgacaaca cggacgttta
     3481 cgccttccgc agcaccgagg cggggcgcga agggttcgtc accttgatcg cgaactacgt
     3541 cccgttccag gagccttcgg gcgggccgca cttctacccg ttcgacgaca acgtcctcta
     3601 cgagatcaag atcgacaaca cgggcgacgg caacgccgac gtgacctacc agatacgctt
     3661 ccgcacggaa gtcaggaacc cggacatcgt cctcggtcag gccgcgccca atcaggcgct
     3721 tgcgggcacc ggcggcatcg agccgctcat cacctcgctc gacgaccccg attacaacga
     3781 gccgcagttc tacaccatca cccgcgtcaa ccgcgcgagc cgcacctcga ccgtgctcgg
     3841 cacaaatctg ccgaccccgc ccgccaacgt cggcgagcgc acgacgccca actacgagac
     3901 ggcgctgggc gcgccggccg tccgcaatct ctcgaacggc ggtcgcgcct tcgccggcca
     3961 gcgcgacgag ggcttctaca tcgacgtggg cgccatcttc gacacgctca acctgcgctc
     4021 gattaccgac acgggcggcg tcgattccac ctcgggcttc aacgtcaaca ccatcgccat
     4081 cgaagtgccg attcaggagc tgacgcgcac gggcacagtc ccggccagct cgaccgccga
     4141 cggcgccgtg gtcggcgtgt gggcgacggc cagccgccgc tcgacgaccg tgctccggcc
     4201 cggcaagcgc gtggtgacgg ggccgttccg tcaggtctcg cgcctcggca acccgctcgt
     4261 caacgaagtc atcatcccgc tcaagctcaa ggacgccttc aactcgctga acccttcggg
     4321 cgacgccgtg gccgcgccct tcgtgctcga cccgcagctc gcgcaactgc tcaaggccgt
     4381 ctttggcatc aacgtccccg cggcgccgcg taacgatctg gtgtccatct tcgccacggg
     4441 catccccgtc aacgccatca cggggccgac ctacacgacc ttcctctcag acggcacgcc
     4501 gcacgagatg atgcgtctca acgtcgccat cgcgcccgcg gccagcccga accgcctggg
     4561 gcttcttggc aacgacgtgg ccggcttccc caacggacgc cgggtcggcg acgacgtggt
     4621 ggacatcgcc ctgcgcgccg tcgccggcgg cacgcccttc acgccggaga ccaactcacc
     4681 gcccaacagc acgctcggcg acggcgttga ccgcaacgac gtgccttacc tgatgcgctt
     4741 cccctatctc ggcacgccgc acagcggcaa caccaagatg ggcaagccgc acgagcacca
     4801 caacgtcatc ccttcgtccg aataatcgag ccagggggga cgggcgggcg gtgctttgaa
     4861 acagcgccgc ccgccttcat ctatcacttg tcatctatcg agagccgcgt ttcgcgtcgg
     4921 aggagagtaa gccgtgaaaa cttctaagct ggtcatgctc gtgtcgctgg gggcggttgt
     4981 cctggcggcg ggcgcgctgg gctttcgaca gtactcgcag aactcggcgg gacgcgcggc
     5041 ggcgtcggcg tcgggtgagt cgtcggcaca gccggcgagt tccgaaaagc agacgccggc
     5101 cgacgtgaga atccgcgcgg gcgaaggcaa gattaagatc gcgcccgaca agccggacgg
     5161 ctacaacctg ctcgcctcgg cctacatgca gaaggcgcgt gagaccggcg actacggcgt
     5221 caacgccaaa gccgtggagg cgcttgagca atcattccgc gtcgcgcccg acaactacga
     5281 cgcgatcaag ctcaacgcca aagtcctcct cacctaccac cgcttcgagg aagggctggc
     5341 cgaagccgag cgcgcgcgga aacagaaccc cgaagaccac gacgtgtacg gcgcgctcgt
     5401 cgatgccaac gtcgagctgg gccgttacga cgcggcggtc gatgccgcgc agcggatggt
     5461 tgacctccgg ccggatgccg ccgcctattc gcgcatcgcc tacatgcgcg agcttcacgg
     5521 cgacactgaa ggagccgtcg aggccatgtc ggtcgccctc aaggccaccg acccgcgcga
     5581 ccccgaggcg gtcgcctggg cgcgtgtcca actcggccac gccctcgcaa gcgtcggcaa
     5641 gcgggccgag gccgagcgcg aatatgacca cgcgcttcgc gtgctgcccg gctacggcgc
     5701 ggcgctcacc tcgaaggccg acgcacgcgc cgcggccggc gacttcgacg ccgcggtaga
     5761 gatttataaa cagttcgatt cacacgacgc acacctcgcc ctcggcgacc tctacgcgcg
     5821 gctcggccgc acggaagatg cggcgcgcga gtacgagtcg ttcgagcggg ccgagcacga
     5881 ggccgccgcc gaggagaacg acatgagcca cctcacgcgc ttctgggccg atcagggtaa
     5941 gaacctcgac gaggcggccg acatcatgcg ccgcgaacgc gtgcggcgcg gagacatcta
     6001 cacctgcgac acgctggcgt gggtgctcta caagaagggc gacctcgccg cggcgcggca
     6061 ggcgattgac gaggcgttgc gtctgggcac gcgcgacgcg aagatcaact accacgccgg
     6121 catgatctat caggccctcg gcgagtcgcg ccgggccgcc cgtcacctca aactcgccct
     6181 cgacatcaac ccctccttcg acgtgcttca ggccgaggct gctcgccgcg ccctcgactc
     6241 gctcaacgtc tgaaggcgcg tgcgcgcggg acgtttcgcg tcttgggctg gcgggcacgg
     6301 cccgccttta aacagtaccg cgagcggtag cgagtgggtg cgactggggg cagtgagtcg
     6361 cctcatcagc gcagacccgc tcgctaccgc tcgcggtact gttttaaccg gccgtaacgc
     6421 gctttagaga ccgaagcgct ccccatatca agtgagttgg cgccccccgc aaatcgtctc
     6481 gtcagcggcg caggtcggaa gatttgcgac gcgtcggtct gcgcccgcgc cggctgcgga
     6541 agggcatgag cgccatccag ccgagggcct tcgcgcgcgg gtgcttgcgg cggccgcgtg
     6601 gtataattgc ttgcatatac aagggtttag cgggcagacg gcggcgagcc gaaaggcccg
     6661 cgtttgcatc taagaagaaa atggagaata gtaggaggcc gtgcgccgaa gcgtcggaag
     6721 tggacgttca aacatatttc agaggggtat cgggaagaga gtaatggcta aaaatctggt
     6781 tattgtcgag tcgccggcga aggcgaaaac catcaacaag tatctcggtt ccgactacaa
     6841 ggtgatggcc tcctacggcc acgtcaagga cttgccgacg aagggtctgg gcgtggacgt
     6901 ggagaacaac tttcagccca cctacgaggt catccccgag acgagaaagc gcaacaacaa
     6961 gaagacgctc gccgaactca agaaggcggc caaagaggcc gaggccatct atctcgcggc
     7021 tgaccctgac cgcgaaggcg aggccatctg ccagcacctc gccgaagaga tcgtgccgaa
     7081 gcgcccggcc aagccgcact tccgcgtgat gttcaacgag attacgaagc gcgccgtgca
     7141 ggcggccttc gaccacccca tgcagatcga tcagcacctc gtcgatgccc aacaggcccg
     7201 gcgcgtgctc gaccgactgg tcggctacaa ggtctcgccg cttttgtgcc gcaccatcgg
     7261 cgggaagctg tcggcggggc gcgtgcagtc ggtggcgctg cgcatggtcg tcgagcgcga
     7321 gcgcgacatc gagaagttca tcaagaccga gtactggacg attgccgcga acgtgtcggc
     7381 caacttgccg cccgccttcg acgcccggct ctacaaggtc ggcgagcaga cggtaaagac
     7441 cggcgcgttc gatcaggacg cgaagaaggg cgagattcac attaaggatg aggcgacggc
     7501 gagtgcgctc gtcgcggaag ccggagggga gcagttcgtc gtcgcggagg tggcgaccaa
     7561 agagcgcaag cgcaaccccg tcccgccctt catcacgtcg aagctccagc aggaggcttc
     7621 gcgcaagctc ggcttcgccg tcaagaagac gatgatgctc gcgcagaaac tctacgaggg
     7681 cgtcgaaatc ggctccgaag gttcggtcgg tctcatcacc tacatgagaa cggactcgac
     7741 gcgcgtctcc gaaacggcgc tcggtgaggc gcgcgatttc gtcggcacgc agtacggcac
     7801 acagtacctg cccgagaagg ccatccacta tcgctcgaag aaggacgcgc aggacgcgca
     7861 cgaggccatc cggccgacgg aagtggcgcg cacgcccgat tccgtggcga gctatctggg
     7921 ccgcgacgag ctgcggctct accggctcat ctggcagcgc ttcgtcgcct cgcagatgat
     7981 gccggccgtc ttcgaccaga cgaccatcga catcagcgcc ggccgcctgc tcttccgcgc
     8041 gaccgggtcg gtcttgaagt tcgacggctt cctccgcgtc tacgaagaag ggcacgacga
     8101 gaagaccgaa gaggacgacg aggcgggccg caagcttccg cccgtcgagc agggcgagcg
     8161 tttgcggctc aacgccgtca cgcccgagca gcacttcacg gagccgccgc cgcgttacac
     8221 cgaggccacg ctggtgaagg cgctcgaaga gaagggcatc gggcggccct cgacctacgc
     8281 gcagatcatg tccacgatca tcgaccgcga gtacgtcgag aaacttgaag gacgcttcca
     8341 tccgacggcg ctggggatga ccgtgaacga cctgttgatt gagggcgggt tcgccgacct
     8401 tttcaacgag acgtacacgg cgcggatgga ggaggaactc gacgaggtcg aggaagggaa
     8461 gatgaagtgg acggaggcgc tcgccgagtt ctacgaaaag ttcgcgcgcg acctcaagga
     8521 gttcgagaaa tatacgaaga cgaagaaggg gatgtcgctc ccgaccgacg aagtgtgtcc
     8581 cgtcgatagc gcgccgatgg tcatcaagtt cggccgcttc ggacagtttc tcgcgtgctc
     8641 caactacccg gagtgccgca cgacgcgcga ggtggcgaag ccgcaggcgg ccgcgggcga
     8701 agccggcgct gaaggcgcgg ccgagggaag tgtccccgcc gccgaggacg aaagctgcga
     8761 gctgtgcggc aagccgatgg ctctcaagcg cgggcgcttc ggacagttcc tcggctgcac
     8821 gggctacccc gagtgccgca acatccgcaa gataagcagg aagggcgtcg tcgctccgcc
     8881 gccggtgccg attgagggcg agacgtgtcc ggtcgatggc gcgcagctcg tccgacgcca
     8941 cggcccctat ggcgagttcg tctcgtgcgc gaattacccc acctgcaagt acatcaagcg
     9001 cgagacgacg ggcgtggcct gcctgcgtcc cggttgtcgg ggcgagctgc tggtgaagaa
     9061 gtcgaagcgc gggaaatact tctacggctg ctcggagtat ccgaagtgcg acgccgtcta
     9121 ctgggacaag cccgtagcct ccccgtgccc gcagtgcaac gcgccgttcg ttttggagaa
     9181 aacgacgaag aagggcacgt tccgcatctg cgcgcgcggc gaggagtgcg gctatcgtga
     9241 ggagcgtctg ccgcaggcgc cgcccgagac gcgcgcgggc gagggcgtga cgccgccgca
     9301 cgccgcgagc tgaagcgagc gcacggtcgg ccggcatttg atttaaccgc ggaggatagg
     9361 ataatttatc catcccccgc ttttcttttc gcacgaaagc aggaaaactt ttaccgcggc
     9421 gtggagtaat aacggactgc cgaccgccgg ccgttcacac acgcggcgaa cgaccgcctc
     9481 ggtgagcggc cgccttcaca gacatatatt tcgagaaagg tgttaacgag atgttgagaa
     9541 aacggatcgc agccctgctg ctctgtacgc tgttcctgac gggtctgccc gcgcaggcgc
     9601 agcaagtggt cgtcgtgaac gcgccgccgc aggcgaaggc cgacccgaac gacccggtcg
     9661 aacgcatcaa ggacgaagga ctgaaccgct cgcaggtgat gcagaccttg tcgtatctga
     9721 ccgatgtcat cggcccgcgc ctgaccaact cgccgcagat gaagcg
//
LOCUS       DASVES010000433        15346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_589782, whole genome shotgun sequence.
ACCESSION   DASVES010000433 DASVES010000000
VERSION     DASVES010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15346
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589782"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VF240_17715"
     CDS             <1..814
                     /locus_tag="VF240_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177100.1"
                     /translation="LRRYDVIVLPHGAPGDYQEMLGESGVARLRRWAEDGGTLVLIKG
                     AAAFATRDGVKWTTATLKRQKLPVRLFFEEGADATDTQRPEQSAPGEARAAREDSQPA
                     PGTGPRTAPPQAATEARGEAQRPAAEPRAAGEGRASEESASAAPVRDVELIRTSGALL
                     RVKVDPQHFLGYGYEADVGATVSSNYAFNISRQGKNVAAYPEEDSLRLAGFVWPESRR
                     ALARTLYLWQERAGRGQVILFADDPNFRAAQLSTMRLFFNAVLLGPSFAGGR"
     gene            1031..2569
                     /locus_tag="VF240_17720"
     CDS             1031..2569
                     /locus_tag="VF240_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7177101.1"
                     /translation="MKRAWKPLLSLTLAAGVLNVSLLSGSVAAQTSAPQTPAARTPAP
                     LRAREKKEQTIARLERLIPQLMAEGDVPGLSVALVRGGELVWHRGFGVRNSATKEPVG
                     PDTVFEAASLSKPVFAYAVLKLVDAGKLDLDAPLNKYLPGNYDVGEDARLAQITARRV
                     LSHTTGFPNWRPRSGADTALKIFFTPGERFSYSGEGFVYLAKAVEQLTGEKFDSFMKR
                     TVFDPLGMTSSSYVWQDRFDALKIFTHNAAGLPAGQNKPERANAAASLQTTAADYGRF
                     VAAVLKGTGLKRATARLMVAPQVKVGAGSNSITRPLGELSPNVSWGLGWGLQTTDDGV
                     AVWHWGDNGNTKAFVVAYPEQKTGVVFFTNSANGLSIARELVGETIGGRQPALDWINY
                     ESYNSPGRTLFKSIMSKGAVAALRDYREWRKGHAAEALDERAMNRLGYDLLNMKKVAE
                     AIEVFKQNVEDYPQSSNVYDSLGEAYMKAGDKEQAIKNYQRSVELDPNNTAGAETLKK
                     LQTP"
     gene            complement(2924..5695)
                     /locus_tag="VF240_17725"
     CDS             complement(2924..5695)
                     /locus_tag="VF240_17725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7177102.1"
                     /translation="MDSERWKKVEEVFEAAADRPEGERAAYLDAACSGDAGLRREVEE
                     LLAHRQPTGALISTLFLDGAKLLRREARVGAEDDDARFLPGDVLAERYRVVGALGRGG
                     MGEVWRADDLKLKQAVALKFLPEKLSKDAEMLGRFHREVRTARRVSHPNVCRVFDIGE
                     TGGSHFLSMEYIDGEDLSSLLRRIGRLPEDKAVEVARQICAGLAAAHDEGVLHRDLKP
                     ANVMIDGRGRARITDFGLAGLADEITGRDISSGTPAYMAPEQLTGKEVSARSDIYSLG
                     LLLYEVFTGRKAFDAPSLDELIRQRETSAPPSISSFIKDVDPVVERVIQRCLESDPSK
                     RPASALQVAAALPGGDPLAAALAAGETPSPEMVAATPREGVLRPAVAVALLASVLVMF
                     AATVMLADRIELYRFVPLEKSPDVLRERAREVAGRFGYASPAADEADGFGLDPKALDY
                     IEANDPSPDRWEKLKTGRPPAIGFWHRGSPRQLVPSSLGAVWWDDPAPIIAGMTTVYL
                     DTRGRLLHFYGVPPQVEEPRGEAEEARGADWAAMLREAGLEPSALKAVESQWAPLYLS
                     DARAAWEGVYPENPAIPIRVEAAAYRGRPVYFEIVHPWDTPARQEEERPTAGRQTLGI
                     LIFGLLALGMVGSALLARRNLRLGRGDRKGAARIAACGFAVGMLLNLSRSHHTPTPDE
                     FFVIINCLAWSIFTALFWWLLYLSVEPFVRRRWPHRIISWTRLVGGDWRDPLVGRDVL
                     VGALAGLTVLVFSTLLARTDWLGGGGPFRPVWQDSSSWLGLSYFGVAFAHQFRTPLVV
                     SLFFIFIMLVLSFPFRRGRAGAFAVWLIITVVWGLIGGATLNAWVGATLAAAILTFVV
                     HRYGLLAMFAAQVFIHHLIFFPTTSDLTAWWAAGFALDLFILLAVIALAFRQSLGGQP
                     LLGRAFPDD"
     gene            complement(5823..6392)
                     /locus_tag="VF240_17730"
     CDS             complement(5823..6392)
                     /locus_tag="VF240_17730"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7177103.1"
                     /translation="MTTTQTHEVTRLLLDWRNGDEGALERLTPLVYAELRRLARRYMR
                     GEREGHTLDGTALVHEAYIRLAGGGGPEWQNRAHFFGVAARLMRQILVEHARRHNADK
                     RGGGERAVSLEESAIFTPARAAGLVALDEALQALARLDPRKSRIVELRYFGGLQIEEI
                     GEVERLSVATVRRQMRMAEAWLHREMSRE"
     gene            complement(6507..7079)
                     /locus_tag="VF240_17735"
     CDS             complement(6507..7079)
                     /locus_tag="VF240_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177104.1"
                     /translation="MNQRLRIAGVLLHVAALIFAVWFGGQVFNALMVVPVWSASLPES
                     AVAYNGALRQFGGGRANFFVLFSPLWVTLLLVAALCLSRRTGPPRRRRWTLAFAACAA
                     VSALVVLGWMAPTVGRGQQAIVEGRFSPEVADALNLWVRANWARLGVELCGALCALRA
                     LGAPAVVSRAREENADEAPGAAVAAVAVVS"
     gene            complement(7130..7300)
                     /locus_tag="VF240_17740"
     CDS             complement(7130..7300)
                     /locus_tag="VF240_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177105.1"
                     /translation="MKSQKKSEERALGYVATACYVFSLALCGVVAYAVVAFALPAFDQ
                     AGQTLYHTGMRF"
     gene            complement(7800..7922)
                     /locus_tag="VF240_17745"
     CDS             complement(7800..7922)
                     /locus_tag="VF240_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177106.1"
                     /translation="MQESHIAACKLLDYQIDQLVYELYDLTPEEIAIVEAGEQH"
     gene            7966..8847
                     /locus_tag="VF240_17750"
     CDS             7966..8847
                     /locus_tag="VF240_17750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX7177107.1"
                     /translation="MSGENGSGNGLHTTLLTEPCTIINGDALAELAKFPDNFFQCCVT
                     SPPYWGLRDYGIEGQLGAEMSLDDYIEKLVLIFSQVRRVLAEDGTLWLNIGDSYTSGN
                     RTWRDNDKKNRARAMNYRPPTPQGLKPKDLVGVPWRVAFALQAAGWHLRSDIIWYKPN
                     CQPESVKDRPTQSHEYLFLMSKSEKYYYDYEAIKEPTKDSNGLRNRRTVWSINTEAFK
                     DAHFAVFPPSLVKPCLLAGSKTNSLVLDPFFGSGTVGEVCLETGRLCVGIELNFEYAR
                     IARNRIAQRANNLAFSY"
     gene            complement(8830..9627)
                     /locus_tag="VF240_17755"
     CDS             complement(8830..9627)
                     /locus_tag="VF240_17755"
                     /inference="COORDINATES: protein motif:HMM:NF036585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177108.1"
                     /translation="MSPASDEDKQERIKHVAKLLPELSESQLGWIEEIVNQFRRPHTF
                     ERNENSSVISECMLGEVGDALRLHHCFSREPFSKDKFEYLLERAANICGTQAELAPKG
                     NPGHDITIGGERYSLKTQANKGLNPKSVHISKFMELGKGQWGAEERDLIGLRDQFLSH
                     MRSYDRILVLRNLSKAPKEWQYELVELPKALLLEAEHGVFEMMQGSKQFPKPGYCHVR
                     DSKGELKFQLYFDGGTERKLQIKRVSKDSCIVHANWRFPAGDLVTES"
     gene            complement(9730..10311)
                     /locus_tag="VF240_17760"
     CDS             complement(9730..10311)
                     /locus_tag="VF240_17760"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7177109.1"
                     /translation="MSFPPQLPAFTQEEYLTLERTSEIRHEYLDGFVCAMAGESPTHS
                     TICFNLAGALHAQLRGTPCRGFSPNMKVRAGEKGLYAYPDLAVVCGEPALHDEHGDVL
                     LNPAVIFEVLSPSTEAYDRGEKFARYRTIETLRDYVLIAQDRPHAEHFSRQPDDTWSR
                     SEVSGEGGVLTLASIDCRVSLADVYERVEFPAA"
     gene            complement(10506..11156)
                     /locus_tag="VF240_17765"
     CDS             complement(10506..11156)
                     /locus_tag="VF240_17765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7177110.1"
                     /translation="MYFSEHFTKTYRERRRVVKSIVVAFVTFLGLALSGTASADTVQY
                     RVEGTFDTGSLIGLSYLEIFTFDDSTRPDVLSSDPWTTEILSYSLNVENHSKHWTLED
                     WPLEQSFSQWVDANGFLNSRAFLATPGPPGDPPGNPPAFSRFFDDGSPNSRSKTVKWY
                     DWSIWGTIQTDSTDLDPTVTVTPVPEPATMILLGTGLAGVGASVRRRRKGDKGRGT"
     gene            complement(11726..12160)
                     /locus_tag="VF240_17770"
     CDS             complement(11726..12160)
                     /locus_tag="VF240_17770"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX7177111.1"
                     /translation="MDRIAKGKWQVRFAALAVFVLGFAAGALALNAYRAWSAGPGRGG
                     EHRRDRFEQMAERLQLSEEQRSQVKQILSETRGQLRALHKESEPRFEEIRRQTDERLQ
                     QVLTPEQFQQFQQMKDEPRRGRGPRGRRGGGDGPPRPPGDER"
     gene            complement(12144..12665)
                     /locus_tag="VF240_17775"
     CDS             complement(12144..12665)
                     /locus_tag="VF240_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177112.1"
                     /translation="MKDQHVIDILESAPLAQLGERELSTVRAHVEACAGCRRAYEATQ
                     ISSLLIRERAAATAFEPSPFFQTRVLAALRERQAALPEVFSLGRLWRAAGGLVSSLAT
                     AVVLLGGLTLFGLMPTTDPTQQSAASADPYSAEAVLFEQAELGEEEINYGQVYSTIYE
                     SDDDAEGVNGQDR"
     gene            complement(12662..13270)
                     /locus_tag="VF240_17780"
     CDS             complement(12662..13270)
                     /locus_tag="VF240_17780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7177113.1"
                     /translation="MELARLARDGDESAFAEIMRRYSPRVFRTAGRFFRRREQVEEAA
                     QEVFLKAFTQLDAYEGRGSFEGWLTRITTTTCLNLLRGAGRRPELTVSDLTDDEGAWL
                     EGKMAGAAAERHRADERGVVAADLAGRVLETMSADDRLVLTMIDGEDAPVKEVAEMTG
                     WSESKVKVRAFRARRRMREAVEKLLGFKGERPDKTLESAGGR"
     gene            complement(13420..13962)
                     /locus_tag="VF240_17785"
     CDS             complement(13420..13962)
                     /locus_tag="VF240_17785"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX7177114.1"
                     /translation="MKRLGKIQTFAIAAVAGLALATPFAIAQTGGGDAQGKDHGKRGE
                     WGGRRGGGGDHRGGGRFGGGMFRGIDLTEEQKTRLQQIHQSFGESTRALREQLRAKHD
                     EVRQAGEGNTFNEQLAAQKLTEAAALQAKLMGEEFRMRQESLSVLTAEQKAQMEQKRE
                     ERRNRRGERRGERRGGGQIL"
     gene            complement(14079..14423)
                     /locus_tag="VF240_17790"
     CDS             complement(14079..14423)
                     /locus_tag="VF240_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177115.1"
                     /translation="MKKRILGITLAVAAVIAGLSATASAQTTYRTRDGRTVTVYPNGR
                     RVYRSPNTGRRVAVRPNGRTIVTLPNGRRVVRSSRSRQYYPQTSQRARTYRLSNGRLV
                     TVYPNGRRVYHR"
     gene            14711..>15346
                     /locus_tag="VF240_17795"
     CDS             14711..>15346
                     /locus_tag="VF240_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177116.1"
                     /translation="MRAFRRIHLAAAALALLCGALAPALGQQQTPGGKLSQARSALVD
                     SAEAHKSSAEELLRLVTEEADRLSATHEQLKQLYADGIVSKKELEESERRLADARARQ
                     ENLRRQMADSDRLIAETEQAEKLAKSRPASSNLIRPSIKFNSTAVVIRYTGRTHSAVA
                     GLGGVQAFFWSTFGRQLPLSAVGQSATHDRFGYDHQHAVDVPLHPDSLEGRA"
BASE COUNT         2900 a         4895 c         4778 g         2773 t
ORIGIN      
        1 tttgcgccgc tacgacgtga tcgtgctgcc gcacggcgcg ccgggcgact atcaggagat
       61 gctcggcgag tcgggcgtgg cgcgcctgcg ccgctgggcc gaggacggcg gcacgctcgt
      121 gctcatcaaa ggcgcggcgg cctttgccac gcgcgatggc gtgaagtgga cgaccgcgac
      181 gctcaagcgc cagaagctcc ccgtccgcct cttcttcgag gaaggcgctg atgcgacaga
      241 cacacagcgc cccgaacaga gcgcgccggg cgaggcgcgc gccgcgcgtg aggactcgca
      301 gcccgcgccg ggcacggggc cgcggacggc gccgccgcaa gcggcaacgg aggcgagagg
      361 ggaggcgcag aggcccgccg cggagccgag ggccgcaggg gaggggcgtg cgtccgagga
      421 gtcggcgtcg gccgcgcccg tccgagatgt cgaactcatc cgcacgtcgg gggcgctgct
      481 cagggtcaaa gtcgatccgc agcacttcct cggctatggt tacgaagcgg acgtgggcgc
      541 gaccgtctcg tcgaactacg ctttcaacat cagccggcag ggcaagaacg tcgcggccta
      601 ccccgaagag gattcgcttc gtctggccgg cttcgtctgg cccgagtcgc gccgggcgct
      661 ggcccgcacg ctctacctgt ggcaggagcg cgccggccgc ggccaagtca tcctcttcgc
      721 cgacgacccc aacttccgcg ccgcgcaact ctcgaccatg cgtctcttct tcaacgccgt
      781 cctcctcggc ccgagcttcg cgggcgggcg ctgagagagg ggcggcgcgg cagggaacac
      841 ggattaaatg gatgagacgg atgagcacgg attcgtgtta atccgtctca tccgtttaat
      901 ccgtgttccg ctccgcgccg ccacaaaatc gccccgcgcg gtgttttaat gacaggcgac
      961 gtgtgtaacg accaaaggaa gtcgcggaca gccgcgcgcc gattaatcca gagacgaagg
     1021 agagacggag atgaaacgag cctggaaacc gcttttgagt ttgacgctcg cggccggagt
     1081 gctgaacgtg tcgcttcttt cggggagcgt cgccgctcag acgagcgcgc cgcagacgcc
     1141 ggccgcgcgc acgccggcgc ccttgcgcgc gcgagagaag aaagagcaga caatcgcgcg
     1201 gctcgaacga ctcatcccgc agttgatggc cgagggagac gtgccgggtc tttcggtcgc
     1261 gctcgtgcgc ggcggcgaac tcgtctggca ccgcggcttc ggcgtgcgca acagtgcgac
     1321 gaaagagcct gtcggccccg acaccgtctt cgaggccgcc tcgctcagca agccggtctt
     1381 cgcctacgcc gtcctcaagt tagttgacgc cggcaagctc gacctcgacg cgccgcttaa
     1441 caaatacctg cccggcaact acgacgtggg cgaagacgcc cgcctcgcgc aaatcaccgc
     1501 ccgccgcgtg ctcagccaca ccacgggctt ccccaactgg aggccgcgca gcggcgccga
     1561 caccgcgctc aagattttct tcacgcccgg cgagcgcttc agctattcgg gcgagggctt
     1621 cgtttatctg gcgaaggcgg tcgagcaatt gacgggggag aagttcgact cgtttatgaa
     1681 gcggacggtc ttcgatccgc tggggatgac gagcagcagc tacgtctggc aggacaggtt
     1741 cgacgcgctg aaaattttca cacacaacgc ggccggcctg cccgcgggcc agaacaagcc
     1801 cgagcgggcg aacgccgccg cgagtctcca gaccacggcg gccgactacg ggcgcttcgt
     1861 cgcggccgtc ctcaagggaa cgggcctcaa gcgcgcaacg gcccggctga tggtcgcgcc
     1921 gcaggtcaaa gtcggcgccg gctccaacag catcacgcgc cccttgggcg agctttcgcc
     1981 gaacgtctca tggggattgg gctggggctt gcaaacaacg gacgacggag tcgccgtctg
     2041 gcactggggc gacaacggca acaccaaggc gttcgtcgtc gcctaccccg aacagaagac
     2101 cggcgtcgtc ttcttcacca acagcgccaa cggcctctcc atcgcgcgcg agctggtcgg
     2161 cgagacaatc ggcggccggc agcccgccct cgactggatc aactacgagt cgtacaactc
     2221 gccgggccgc acactcttca agagcatcat gagcaagggc gccgtcgcgg ccctgcgcga
     2281 ttaccgcgag tggcgcaagg ggcacgccgc tgaggcgctc gacgagcgcg cgatgaaccg
     2341 tctcggctac gacttgctca acatgaagaa ggtggccgag gccatcgagg tcttcaaaca
     2401 gaacgtcgaa gactacccgc agtcgtcgaa cgtctacgac agtctgggcg aagcctacat
     2461 gaaggccggc gacaaggaac aggccatcaa aaactaccaa cgctccgtcg agcttgaccc
     2521 caacaacacg gccggcgccg agacgttgaa gaaacttcaa acgccgtgag cggcgcagta
     2581 ccgtggcaag cagcggtttg caaagcccca aaggggcgaa atgagatagc caggggcagc
     2641 gcccctgaat aatcggcggg agtaattcgt aagccccgaa ggggtgaaat atccacgctg
     2701 ggtatttcac cccttcgggg cttaataaac acacctcact caccgggcag cgcgtcctgc
     2761 gcggcagctt cctcgaagac tgaggcggct tttgactgcc tgtgtggttg agtgccgtcg
     2821 cgcggccggg gaacacggat aggacggatg ggacggatgg acgcggattc gtgcttatcc
     2881 gcgcttatcc gtttaatccg tgttactgat gtgcgaatcg tgctcagtcg tcggggaacg
     2941 cgcggccgag gagcggctgg ccgccgaggc tctggcggaa ggcgagggca atgacggcga
     3001 ggaggatgaa gaggtcgagg gcgaagccgg cggcccacca cgccgtgagg tcgctcgtcg
     3061 tggggaagaa gatgagatgg tggatgaaga cctgcgcggc gaacatggcg agcaggccgt
     3121 agcggtggac gacgaaggtg aggatggccg cggcgagcgt cgcgccgacc cacgcgttga
     3181 gcgtggcgcc gccgatgagt ccccagacca cggtgatgat gagccagacg gcgaaggccc
     3241 cggcgcggcc ccggcggaac gggaacgaga gcacgagcat gatgaagatg aagaagagcg
     3301 agacgaccag cggggtgcga aactggtgcg cgaaggcgac gccgaaatag ctcagcccga
     3361 gccaactgga cgagtcctgc cagacgggcc tgaaggggcc gccgccgccg agccagtcgg
     3421 tgcgcgccag cagcgtggag aacacgagga cggtgaggcc ggcgagcgcg ccgaccagca
     3481 cgtcgcgccc gacgagcggg tcgcgccagt cgcccccgac gagacgcgtc caagagatga
     3541 tgcggtgcgg ccagcgccgc cggacgaacg gctcgacgga gagatacagg agccaccaga
     3601 agagtgcggt gaagatgctc cacgcaaggc agttgatgat gacgaagaac tcgtcgggcg
     3661 tcggcgtgtg gtgggagcgc gagaggttga gcagcatgcc gacggcgaac ccgcaggccg
     3721 cgattcgcgc cgcgcccttg cggtcgccgc gtccgaggcg caggttccgg cgcgcgagca
     3781 gagcgctgcc gaccatcccc agcgcgagca agccgaagat caggatgccg agtgtctggc
     3841 ggcccgccgt cggacgctcc tcctcttgtc gcgccggcgt gtcccacggg tggacgatct
     3901 cgaagtagac gggacggccg cggtaggcgg ccgcctcgac gcgaatggga atcgccgggt
     3961 tctccgggta gacgccctcc cacgccgcgc gcgcgtcgct cagatacagc ggcgcccact
     4021 gcgactccac ggccttgagc gccgacggtt cgagaccagc ctcgcgcagc atcgccgccc
     4081 agtcggcccc gcgcgcctcc tcggcctcgc cacgcggctc ctcgacctgc ggcggcacgc
     4141 cgtagaagtg cagcaggcgg ccccgcgtgt cgaggtagac ggtcgtcatc cccgcgatta
     4201 tcggggcggg gtcgtcccac cacacggccc cgaggctcga cgggacgagt tgtcgcgggc
     4261 tcccgcggtg ccagaagccg atggccggcg ggcggcccgt cttcaacttc tcccaacggt
     4321 cgggcgatgg gtcgttcgct tcgatgtagt cgagggcctt cgggtccaat ccgaagccgt
     4381 cagcctcgtc cgcggcgggc gacgcgtacc cgaaccgccc ggccacctcc cgcgcgcgct
     4441 cgcgcagcac gtcgggcgac ttctccagcg gcacgaacct gtacagctca atcctgtccg
     4501 cgagcatgac ggtcgcggcg aacatgacga ggacggatgc cagcagcgcg acggccaccg
     4561 ccggcctgag cacgccctcc ctcggcgtgg cggcgaccat ctcgggcgaa ggcgtctccc
     4621 cggccgccag cgccgccgcc agtgggtcgc cacccggcag cgccgccgct acttgcagcg
     4681 ccgacgcggg acgcttcgac gggtcagatt cgagacatcg ctggatgacg cgctcgacga
     4741 cggggtcaac gtccttgatg aaagatgaaa tgctcggcgg cgcgctcgtc tcgcgctggc
     4801 ggatgagttc gtcgagcgac ggcgcgtcga acgccttgcg gcccgtgaag acttcgtaga
     4861 gcaggaggcc gaggctatag atgtcgcttc gggccgagac ctctttgccg gtcagttgct
     4921 cgggggccat gtaggcgggc gtccccgagc tgatatcgcg gcccgtgatt tcgtcggcga
     4981 gtccggccag cccgaagtcg gtgatacgcg cgcggccccg gccgtcaatc atcacgttgg
     5041 cgggcttgag gtcacgatga agcacgccct cgtcgtgcgc cgccgctagc cccgcgcaga
     5101 tttgtcgcgc cacctcgacg gccttgtcct cgggcagccg gccgatgcgc cgcagcaggc
     5161 tggagaggtc ttcgccgtcg atgtactcca tcgagaggaa gtgggagccg cccgtctcgc
     5221 cgatgtcgaa gacgcggcag acgttcgggt gcgacaccct gcgggcggta cgcacctcgc
     5281 ggtggaagcg acccagcatc tcggcgtcct tcgacagctt ctcgggcagg aacttgagcg
     5341 ccaccgcctg cttgagcttg aggtcgtcgg cccgccagac ctcgcccatc ccgccacggc
     5401 cgagcgctcc gacgacgcga tagcgttcgg ccagcacgtc gccgggaagg aagcgcgcgt
     5461 cgtcgtcttc cgcgcccacg cgcgcctcgc ggcgcagcag tttggccccg tcgaggaaga
     5521 gtgtggagat gagcgcgccc gtgggctgcc ggtgcgcgag cagttcttcg acctcgcgcc
     5581 gcagccccgc gtcgcccgag cacgccgcgt cgagatacgc cgcgcgctcc ccttcggggc
     5641 gatccgccgc cgcctcgaac acctcctcaa ctttcttcca gcgttcgctg tccataagct
     5701 tcgcgtgata agtgatgagt gataagtgat aagaaagaaa agaagccatt agcttttagc
     5761 tgttggctgt tagctaagcg ctaagagcta acagcttctt ctcatttatc acttatcact
     5821 tatcactctc tcgacatttc ccgatgcagc cacgcttcgg ccatcctcat ctgccggcgg
     5881 acggtggcga ccgacaagcg ctcgacctcg ccgatctctt cgatctggag gccgccgaag
     5941 tagcgcagct cgacgatgcg gctcttgcgc gggtcgagcc gggcgagtgc ttgcagcgcc
     6001 tcgtcgagcg cgacgagacc ggcggcgcgc gcgggcgtga agatggccga ctcttcgagc
     6061 gacacggcgc gctcgccgcc gccgcgcttg tccgcgttgt gccggcgcgc gtgctcgacg
     6121 agtatctgcc gcatcagccg cgcggccacg ccgaagaagt gcgcgcggtt ctgccactcg
     6181 gggccgccgc cgccggcgag ccggatgtat gcctcgtgga cgagcgccgt gccgtcgagc
     6241 gtgtggccct cgcgctcgcc gcgcatgtag cggcgcgcga ggcggcgcag ctccgcgtag
     6301 acgagcggcg tcagacgctc aagcgcgccc tcgtcgccgt tgcgccagtc gaggagcagg
     6361 cgcgtcactt cgtgtgtctg ggtcgtggtc atccgaacgg ccgtgtccgc gcctcgcctc
     6421 cgcgccgggc cgcgcggggc ggagggcgac gagagcttat tgattgcggc gggggccgtc
     6481 aacagattcg tcgcacgtcg ccggctttaa ctgacgaccg cgaccgccgc gaccgccgcc
     6541 cccggcgctt cgtccgcatt ctcttcccgc gcgcggctca cgacggccgg cgcgccgagc
     6601 gcgcgcaacg cgcacaacgc gccgcacagt tccacgccga gccgcgccca gttggcacgc
     6661 acccacaggt tgagcgcgtc ggcgacctcg gggctgaagc gcccttcgac gatggcctgc
     6721 tggccgcgtc ccacggtcgg ggccatccag ccgaggacga ccagcgcgga gacggcggcg
     6781 caggccgcga aggcgagcgt ccaacgccga cgccgcggcg gccccgtcct gcggctcaga
     6841 cacagcgcgg cgacgagcag cagtgtgacc cacaacggac tgaacagcac gaagaagttc
     6901 gctcggcctc caccgaactg cctgagcgca ccgttgtagg cgacggccga ctcgggcagg
     6961 ctcgcgctcc agacaggcac gaccatcagc gcgttgaaca cctgcccgcc gaaccacacg
     7021 gcgaagatga gcgccgccac gtgcagaagc acccccgcga ttctgagtct ctgattcaca
     7081 cacacctcca ctgcggctgc gccggctttg tgcccgccgc cgcctctggc tagaacctca
     7141 tgcccgtgtg gtataaggtc tgcccggcct gatcgaacgc cggcagcgcg aacgccacga
     7201 cggcgtaggc gacaacgccg cacaacgcca gcgagaacac gtaacaggcc gtggcgacgt
     7261 agcccagcgc ccgctcctcg gatttctttt gactcttcat caccgcctcc ttcttccatc
     7321 tcggctgcgc ccgccgcgcg ccggaaaata acttcctcgc cccgtaatgc gcgccccgga
     7381 ggccgaatcg ctcacacccc gaaaaattat tttcgccggc ccccgaagaa aaaaggagtg
     7441 ggcgacccgc gcgtgtgggc cgcccactcc ttacttaacc ccagtcttga gttgaaaaat
     7501 ggctttgttg atttcaaagg actaacggca tcaagtccaa ctcaagactg cgaatcagtg
     7561 ccagaagtcc tttgtttggg cgattcgttt tcaactcaag actggggttt agttacgaag
     7621 gttcggaaca cagttcgggc taatgtgagg agagtttcga tgaccggact gcaaacacca
     7681 cgacgttgaa acagtaatcc acgaagtcac acgaagcggc acgaaacaga tttaacccgg
     7741 cgcagcttca gccccttttc gtgtcgcttc gcgtgtcttc gtggattgtc cgcgccgcat
     7801 caatgctgct cgcccgcctc gacaatggca atctcctcgg gcgtgaggtc gtagagttcg
     7861 taaacgagtt ggtctatctg gtagtcgagc aatttgcacg cggctatatg tgattcctgc
     7921 acctgaatac ttgtattaga agcttttgga gctattatga gatgtatgag cggcgagaac
     7981 ggaagtggta acggactcca tacgacactg ctaaccgagc cctgcacaat tatcaacggc
     8041 gatgcgttgg cggagcttgc caaatttccc gacaactttt ttcagtgttg cgttacaagc
     8101 ccgccttatt ggggactacg agactatggc atagaaggtc agctaggggc ggaaatgtcg
     8161 ctcgatgact atatcgaaaa gcttgtactg atcttcagtc aagtgcgtag agtattagca
     8221 gaagatggca ccttatggct gaacattgga gattcgtaca cgagtggcaa cagaacgtgg
     8281 agagataacg ataaaaagaa tcgcgcgcgc gcgatgaatt accgaccacc caccccacag
     8341 ggattaaagc cgaaagactt ggttggcgtt ccgtggcgcg ttgcatttgc cctccaagcg
     8401 gcgggttggc atcttcgctc agatataatc tggtacaaac ctaactgcca acccgaatct
     8461 gtgaaggata gaccgacgca atcacatgaa tacctcttcc tcatgtcgaa gtcggaaaag
     8521 tattattatg actatgaagc tatcaaagaa ccgactaaag atagtaatgg tctgaggaat
     8581 aggcgcacgg tatggtcaat taatactgag gcctttaaag acgcccattt tgccgtattc
     8641 ccaccatcat tagttaagcc ctgtcttctc gcagggagca agacaaattc tttagtgctt
     8701 gaccctttct ttggctcagg gacagtcggc gaggtgtgtc ttgaaactgg caggttatgc
     8761 gtcgggatag agctaaattt tgaatacgcg agaatcgccc ggaacagaat agcgcagagg
     8821 gctaataatt tagctttcag ttactaagtc accggccggg aagcgccagt ttgcgtgaac
     8881 gatacaacta tctttactta cccttttgat ttgtaatttg cgctcagtcc caccatcgaa
     8941 atagagttgg aatttcaatt caccttttga atctctcaca tggcaatacc cgggcttagg
     9001 gaattgctta ctaccctgca tcatctcaaa aacaccatgc tctgcttcga gtaacagagc
     9061 cttcggtaat tccaccaatt catactgcca ttctttagga gccttagaca ggttacgcag
     9121 gacaaggatt cgatcatatg atctcatgtg agacagaaat tgatcccgaa gccctatcag
     9181 gtcacgttcc tcggctcccc attgtccttt gcccaactcc atgaattttg aaatatgcac
     9241 agacttgggg ttgagacctt tattagcttg agttttcagc gaatacctct ccccacctat
     9301 tgtaatgtcg tggccgggat ttcctttagg ggcaagttct gcctgagtgc cgcaaatgtt
     9361 tgccgcccgc tctaagagat actcaaactt atcctttgaa aatggctcgc gagagaagca
     9421 gtgatgcagg cgaagcgcgt cacctacttc tccgagcata cattcagata tgacacttga
     9481 gttttcatta cgctcgaatg tatgcggcct cctgaactga ttgactatct cctcaatcca
     9541 acccagttgg ctttcggaga gttccggtag cagcttggct acgtgtttga ttcgctcttg
     9601 cttatcttcg tcagaagcag gtgatattga agattctccg acccatcact gacgcgagta
     9661 gatcagcccc gtttgaccct gatatctgcc accgcagtct ttgaactgtt cgaagtaata
     9721 atgcgccttt caggcggccg ggaattcaac ccgctcgtaa acgtccgcga gtgagacgcg
     9781 gcaatcaatc gacgcgagcg tgaggacgcc gccctccccg ctcacttcgg agcgcgacca
     9841 agtgtcgtcg ggctggcgtg agaagtgttc ggcgtggggc cgatcctgcg cgatgagcac
     9901 gtagtcgcgc agcgtctcaa tcgtgcggta gcgcgcgaac ttttcgccgc ggtcgtaggc
     9961 ttcggtcgag ggcgagagga cttcaaagat gaccgccggg ttaagcaaca cgtcgccgtg
    10021 ctcgtcgtgg agggcaggct ctccgcagac gacggccaga tcagggtagg cgtagaggcc
    10081 cttttcgccc gcgcggactt tcatgttcgg ggagaagccg cggcagggcg tgcccctcaa
    10141 ctgcgcgtgc agggcgccgg ccagattgaa gcagatggtg ctgtgggtcg ggctttcgcc
    10201 cgccatcgcg cagacgaaac cgtccagata ttcgtggcgg atttcggacg tgcgttcgag
    10261 tgtcaaatac tcttcctgcg tgaaggccgg aagctggggc gggaaggaca tcgtcactcc
    10321 tcaatcgccg gcgaacgggc cgacgcgtcc gccccgaagt ataaaccacg ccgcccgaaa
    10381 acctcacaga tgcgaatggc tggtagtggg tcattttcaa tgttgaacaa aaagtaagag
    10441 ggcggcgcga gtggccgccg ccctcttaac actatgaggt gttcggtttc gcgtgccccg
    10501 atgcgctacg tcccccggcc cttgtcacct ttgcgccgcc tgcggacaga cgcgccgact
    10561 cctgccagtc cggtgcccag gagaatcatt gtggcgggct ccgggacggg agtgactgtg
    10621 acagttgggt ctaagtcggt ggaatcagtc tgaatagtgc cccatatact ccagtcgtac
    10681 catttcacgg tttttgatct ggaatttggt gaaccatcat cgaagaatct tgaaaaggct
    10741 ggaggatttc ctggaggatc tcccggcggt cccggcgtag ccaaaaaggc acggctgttc
    10801 aaaaatccgt tggcgtcaac ccactgagag aacgattgct ccaatggcca atcctcaagg
    10861 gtccagtgtt tgctgtgatt ttccacattg aggctatagc tcaagatctc ggttgtccac
    10921 ggatcgctac ttagcacatc aggcctcgtg gagtcatcaa atgtgaatat ttccaagtag
    10981 cttaggccga taagactgcc ggtgtcgaaa gtaccctcga ccctgtattg cacagtatca
    11041 gcactagcgg tgccgctaag ggcaaggccg agaaaggtta caaacgccac gacgatggat
    11101 ttgacaaccc tccggcgttc acgatacgtt ttggtaaaat gttctgagaa gtacataact
    11161 aacctcctaa attgcttgat gctctcgttc gcctgctcag gggcaggtcc gcatttgaga
    11221 tgaggcggta aacaataaag ccctccgctg ctgtgaacac acttcgccca agaaagcagc
    11281 attcctgctt ttcgatagca agtgtgtcac agtggatgag ggccgcacga ttcacccttt
    11341 atgtgactga tcgttgatgt aaagctggcg ggagtgggag tgtctctcgt caacgtgagg
    11401 tgtcgatctg gcgggtgcga ggcactcgcc ggaacgaaca cagggggatg aggacagcat
    11461 atactcttat cgcggaaggg tgtcaagata aaaggcggcg ggtcattttc aacgccggac
    11521 aagaacgagg atggaactgg ggaagaagct agggcatact cttctttagg tgttcaacgt
    11581 tgtaaatgac ccactaccga atggctttgc acaaatgttc gtgaaccttg aacatttgtg
    11641 caaagccggt gtaactacgg cttcgtgtct tttagcgagg cttcgtggtt aatgccttat
    11701 ctctactcgc gcgactcgct cccgcctaac gctcgtcgcc cggcgggcgg ggagggccgt
    11761 cgccgccgcc ccggcgtccc ctcgggccac ggcctcgccg cggctcgtcc ttcatctgtt
    11821 gaaattgctg aaactgctcc ggcgtgagca cctgttgcag gcgctcgtcg gtctgccggc
    11881 gaatctcctc gaagcgtggc tccgactcct tgtgcaacgc gcgcagttgc ccacgcgtct
    11941 cggagagaat ctgtttgacc tgcgagcgct gctcctcgct cagttgcaga cgctcggcca
    12001 tctgctcgaa gcggtcgcgt cggtgttcgc cgccgcgccc cggcccggca gaccacgcgc
    12061 ggtaggcgtt caaggcgagt gcgcccgccg cgaacccgag cacgaagacg gcgagcgccg
    12121 cgaagcgcac ctgccacttg cccttagcga tcctgtccat tgacaccctc cgcgtcgtca
    12181 tccgattcgt agatggtcga gtagacctgt ccgtagttaa tctcttcttc gcccagctcg
    12241 gcctgctcga agagcacggc ctcggccgag taggggtcgg ccgacgcggc cgactgctgc
    12301 gtcgggtccg tcgtgggcat caggccgaag agcgtcaggc ccccgaggag cacgacggcc
    12361 gtcgccagcg aggagacgag cccgccggcc gcgcgccaca gccggcccaa cgaaaagact
    12421 tccggcaggg ccgcctgccg ctcgcgtagc gccgccagca cgcgcgtctg aaagaagggc
    12481 gacggctcga acgccgtcgc ggccgcacgc tccctgatga gcagcgacga gatttgcgtc
    12541 gcctcgtagg cgcgtcggca cccggcgcaa gcttcgacgt gtgcgcgcac cgtcgaaagc
    12601 tcgcgctcgc ccaactgcgc gagcggggcg ctctcaagga tgtcgataac gtgttggtct
    12661 ttcatctccc acctgcgctt tccaaagtct tgtccggcct ctcccccttg aaccccagca
    12721 gcttctccac cgcctcgcgc atccgtcgcc gcgcccgaaa cgcccgcacc ttaactttcg
    12781 attccgacca gcccgtcatc tccgccacct ctttgacggg ggcgtcttcg ccgtcgatca
    12841 tcgtcagcac cagccggtcg tctgccgaca tggtctccag cacgcgcccg gccaaatcgg
    12901 cggccacgac gccgcgctcg tcggcgcgat gcctttcggc ggccgcgccc gccatcttgc
    12961 cctccagcca cgcgccctcg tcatcagtca aatccgagac ggtcaactcg ggccgtcgcc
    13021 cggcgccgcg cagcaggttg aggcacgtcg tcgtggtgat gcgcgtcagc cagccctcga
    13081 aggagccgcg cccctcgtag gcgtcgagct gcgtgaacgc tttcaaaaaa acttcctgcg
    13141 cggcctcttc gacctgctca cgccggcgga agaagcggcc cgccgtgcgg aagacgcgcg
    13201 ggctgtagcg cctcatgatc tcggcgaagg cagactcgtc gccgtcacgc gcgaggcgcg
    13261 ccagctccaa gtccgttgcc cgttcaagcc catccggcag aggcgcaccc cgatagattg
    13321 ggtgatgagt gatgagtgat gagtgataag aaagagaaag cgaatttgtc cttcttatca
    13381 cttatcactc atcactcatc acttcttgaa aagtgagcgt cagagaatct gaccgccccc
    13441 tcgacgctcg ccgcgacgct cgccgcgacg gttcctgcgc tcctcgcgct tctgctccat
    13501 ctgcgctttc tgctccgccg tgagcaccga cagcgactcc tgccgcatgc ggaactcttc
    13561 gcccatcagc ttagcctgaa gcgcggcggc ctcggtcaac ttctgggccg cgagctgctc
    13621 gttgaaggtg ttgccctcac cggcctgacg cacctcgtcg tgcttggcgc gaagctgctc
    13681 gcgcagcgcc cgtgtgctct ccccgaagct ctggtgaatc tgctggaggc gcgtcttctg
    13741 ctcctccgtc aggtcgatgc cgcggaacat cccgccgccg aagcgtccgc cgccgcgatg
    13801 atcgccgccg ccgccgcgac gcccgcccca ctcaccacgc ttgccgtgat ctttaccctg
    13861 agcgtcgccg ccgccggtct gggcgatggc gaaaggcgtg gccagtgcga ggcccgccac
    13921 ggcggcgatg gcgaacgtct gaatctttcc gagtcttttc attttggttt cgtgctcctt
    13981 agtttacctg tgttcttgtc ttctgaggag agcctcttaa ggaaaagaca ccgggccgcc
    14041 cccgcgggtt accgacccgg cgccctcgcg tgattgcttt agcgatgata gacgcggcgc
    14101 ccgttcgggt aaacggtgac gagtctcccg ttggagagac ggtaggtgcg cgcgcgctgc
    14161 gaagtctgcg ggtagtactg ccggctgcgc gagctgcgga cgacacgccg gccgttcggc
    14221 agagtgacga tggtgcgccc gttcgggcgg acagccacgc gccgccccgt gttcgggctg
    14281 cggtagacgc ggcgtccgtt cgggtagacg gtcacggtgc ggccgtcgcg cgtccggtac
    14341 gtggtctgcg ccgaggcggt cgccgacagg cccgcgatga cggccgcgac ggcgagtgta
    14401 atccccaaaa tccttttctt cattttcttc tcctcttgct tggttcaggg gggagaccct
    14461 gtgatttatg actgcgggga gaccgaccta atgacttcct ctcgcttgga caactcgcgg
    14521 aagccttctg aacaggtaag acacccgaaa ccctcgcggg gttacaggcg ttcttaattc
    14581 tttacgcgcc ggttagaaat gggcgtcgtc tgcatctaat acggacgctc gaattgatga
    14641 gtaaaccgcc gctgtaacgt cgcgcattta aacggtgtct atcgacttgg ggaggatttc
    14701 cgagccgccg atgagagctt tcagacgcat acatttagcc gccgccgcgc tcgcgctttt
    14761 gtgcggcgcc ctcgcgccgg cgctcgggca gcagcagacg ccgggcggca aactctcaca
    14821 ggcgcgctcc gctctcgtcg attcggccga ggcgcacaag tccagcgcgg aggaactgct
    14881 ccgactcgtc actgaggagg ccgaccgtct ctccgccacg cacgaacagc tcaagcagct
    14941 ctacgccgac ggcatcgtct ccaaaaaaga gcttgaggag agcgaacgcc gcctcgccga
    15001 cgcccgcgcc agacaggaga atttgcgccg ccagatggcc gactccgacc gtctcatcgc
    15061 cgagaccgag caggccgaaa aactcgcgaa atcgcggccc gcgtcgtcga atctgattag
    15121 gccgtcaatt aagttcaact cgacggcggt cgtcattcgc tacaccggca ggacacactc
    15181 ggcggtcgcg ggcctcgggg gcgtacaggc tttcttctgg tccacgttcg ggcgtcagct
    15241 ccccttgagc gccgtgggac aatcggcgac gcacgaccgc ttcggctacg accaccagca
    15301 cgcggttgac gtgccgctgc accccgacag cctggagggc cgcgcc
//
LOCUS       DASVES010000434         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1384629, whole genome shotgun sequence.
ACCESSION   DASVES010000434 DASVES010000000
VERSION     DASVES010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384629"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..442
                     /locus_tag="VF240_17800"
     CDS             101..442
                     /locus_tag="VF240_17800"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX7177117.1"
                     /translation="MLPESDEDLLRECEVDTFRSSGPGGQHVNKTESAVRLRHLPSGV
                     VVTSQQERSQHRNKALCLQKLRERVRQLNHRPAKRVPTRVPRGAKTRTLEAKARRSRV
                     KRLRSKPSADE"
     gene            498..854
                     /locus_tag="VF240_17805"
     CDS             498..854
                     /locus_tag="VF240_17805"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX7177118.1"
                     /translation="MITVANRIYVNAEYGEAFEQRFRERAGLVDQMPGFVSNQVLRPV
                     NDGDPYVVFTVWESRQDFLNWVRSDAFTKGHAQSGTLPKEAFSRANVLEMHEVVQDSA
                     RPDLEPEKPGGPFKMH"
     gene            complement(1077..>2097)
                     /locus_tag="VF240_17810"
     CDS             complement(1077..>2097)
                     /locus_tag="VF240_17810"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HEX7177119.1"
                     /translation="KEHGVATPHDYADLAALIRAGELVEVPTVGDSYVLFGVGGSADK
                     QLFTHYDKTAGESVALLDDAELAREYERTDERVEALRAEIGALRKELDSLGKGERSRR
                     AGLQRQIAQQEKTLKAEREGRELLNDHYGKEGRRRELAAERDALAALAADFRGRGYDL
                     ADARSRLEMKVRMLSHLRPEALAVLREFADSYHAKFGRPLPVTSLVRPDEYQRRLSAT
                     NANATRIDTPPHSTGLAFDILYRHMTAEEQAHVMSDIARLRDAGRVEALRENRDHFHV
                     FAFIDGKRPGEELISGSLGRAAAAAPKAEPARVPKKELKAEKKKAVKKEVEKRGGRTA
                     GRKRR"
BASE COUNT          349 a          652 c          692 g          404 t
ORIGIN      
        1 ttaacgtccg ccggccggct cgctatcagt gcggctgatt ctggtagggt atgattgcga
       61 ccgtgacggc catcccggcc gcgggggttt gaacatgatc atgctgccgg aatcagacga
      121 ggacttgttg cgcgaatgtg aggttgacac cttccgttcg agcggccccg gcggccagca
      181 cgtcaacaag acggaatctg ccgtccggct caggcacctg ccttcgggcg tcgtcgtcac
      241 gtcccaacag gagcgcagcc agcatcgcaa caaggcgctg tgtttgcaaa agctccgcga
      301 gcgagtccgt caacttaatc accggccggc caagcgcgtc cccacgcgcg tcccacgcgg
      361 cgcgaagacg cgcacgctcg aagcgaaggc gcggcgctcg cgcgtcaagc gtctgcgctc
      421 gaagccgtcg gcggatgagt gagcgggaac gggagcggct gctgtagtgt tactatgagg
      481 tcaagggaga tttgggaatg ataactgtcg ccaatcgtat ttacgtcaac gcggagtatg
      541 gggaagcctt cgagcagagg ttccgagagc gggccggact cgtggatcag atgcccggct
      601 tcgtctccaa tcaggtgcta cggcccgtca acgacggcga cccctacgtg gtcttcaccg
      661 tctgggagag ccggcaggac ttcctcaact gggtgcgctc cgacgccttc acgaaggggc
      721 acgcgcagtc cggcaccttg ccgaaagaag cattctccag agctaacgtg ttggagatgc
      781 acgaggtcgt ccaggattcg gcacgtccgg acttggagcc ggagaagccg ggcgggccgt
      841 tcaagatgca ctagagcagg tcttcgtttg aatttacagg gtagggttta gagcggtgat
      901 tactggaatg aaagcgacat agattcaccg cggagtcgca gaggacgcgg agttcgcgcg
      961 gaggattgaa tcaactctcc gcgtaaactc cgcgtccttt gcgactccgc ggtgagtcag
     1021 acttaccatt cactcaactg atgattgcaa cggaacagtc taagacaggc tgaatcctac
     1081 ctccgctttc tccccgccgt ccggccgccc ctcttttcga cttctttctt cacggccttc
     1141 ttcttctcgg ccttcaattc ttttttagga acacgcgccg gctccgcctt cggggccgcc
     1201 gccgccgccc ggccgagcga accgctgatg agttcttcgc cgggacgttt gccgtcgatg
     1261 aaggcgaaga cgtggaagtg gtcgcgattc tcccggagcg cctcgacgcg gccggcgtcg
     1321 cgcagacgcg cgatgtccga catcacgtgc gcctgctctt cagccgtcat gtgccggtag
     1381 aggatgtcga aggcgagacc cgtggagtgc ggcggcgtat cgatgcgcgt ggcgttggcg
     1441 ttcgtcgcgc tcaggcggcg ctggtactcg tcgggacgca cgagcgaggt gacgggcagg
     1501 ggccgtccga atttcgcgtg ataggaatcg gcaaactccc tgagcaccgc cagcgcctcg
     1561 ggccgcaggt ggctgagcat ccgcaccttc atctccagac gcgagcgcgc atccgcgagg
     1621 tcgtagccgc ggccacggaa gtcggcggcg agtgcggcga gtgcgtcgcg ctcggcggcg
     1681 agttcgcggc gtcggccctc tttgccgtag tggtcattta atagctcgcg tccttcgcgc
     1741 tccgccttga gcgtcttctc ctgctgtgcg atctgccgct gcaagccggc gcggcgcgag
     1801 cgctcgcctt tgccgagcga gtccagttct ttcctgagcg cgccgatctc cgcgcggagg
     1861 gcttcaaccc tctcgtccgt gcgctcgtac tcgcgcgcga gttccgcgtc gtcgaggagc
     1921 gcgaccgatt cgccggccgt cttgtcgtaa tgtgtaaaga gctgcttgtc ggcgctgccg
     1981 ccgacgccga agaggacgta gctgtcgccc acggtcggga cttcgaccag ctcgccggcg
     2041 cggatgagcg cggcgaggtc ggcgtagtcg tggggcgtgg cgacgccgtg ctccttc
//
LOCUS       DASVES010000435        23333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_635183, whole genome shotgun sequence.
ACCESSION   DASVES010000435 DASVES010000000
VERSION     DASVES010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635183"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VF240_17815"
     CDS             <1..922
                     /locus_tag="VF240_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177120.1"
                     /translation="QQVSSAVDEAAQQVQQAAQQAAQQVGQAVDQAAQAAQRAGELAT
                     QAAQAIQQQAQRAAAQAADQIAGAAEAAFDAVNDVMSQLPSADDALNQLQRAVTDVTN
                     DFPQTPPSPTAACPFAVSPERLTEQVNAARNRAEQSLQQAQAAITQTAQRAFEQAQQH
                     AGQVADQIQNSVQAAVAQVQQATEQAVDQARQTTEQALQQVQQTAQEGAQAAQQAIQQ
                     AQQQVSSAVDEAAQQVQQAAQQAAQQVQQQAQQAVQQAQQAGAMAAQAAQDAQQQVAA
                     TTRQAAQSVADTAAESLSSVNQAMSQLGLS"
     gene            1022..2611
                     /locus_tag="VF240_17820"
     CDS             1022..2611
                     /locus_tag="VF240_17820"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX7177121.1"
                     /translation="MAEPISLEKPEFNLVASTEETESSAAATETTPDTSFRIALLGDF
                     AGRASRANATPSGRRLFEIDRDNFDTVLRNLGVELSLSFPAAAAPVALRFAELEDFHP
                     DRIYERLDIFRSLRETRGRLADPSTFDAAAAEVRGWADPAAASPRREPTEAADPQRAP
                     AADTPGRDLLEQMLGRAASADAPSRSPSERPAELTDFGRFLGDIVRPHLASVDEGQKA
                     RLTEAVDEAAAELMRAILHHPDFQSLEAAWRATHFLVSRTETDENLKLFLLDFTREEL
                     TEGLRAGEDARGGRLGELLAGHSGHTHADEPWALLVGNYTFDRTPEDAELLQLISFVA
                     RRARAPFVAAASTRAAGLESFFAEPSDESLPEGDVGEVAPEEWASLRRLPEARYLGLA
                     GPRFLLRLPYGAETDAAERFDFEEMGAGDEPRHGEYLWGNPAFACAHLLAEAFGRAGW
                     GMRAGDVKEIEGLPLHVHEVQGETRNKPCAEVVLTERAAAEVLERGVMPLLSFKNADH
                     VRLARFQSLAQPPTPLAGRWS"
     gene            complement(2608..2949)
                     /locus_tag="VF240_17825"
     CDS             complement(2608..2949)
                     /locus_tag="VF240_17825"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7177122.1"
                     /translation="MQAQVNEYVVGRTPLMLAAFEGKIATVQVLLRRGADVNARDGDG
                     NTALMFAAFGGHAAIVHLLLERGAEVDAEARDGWTAVGAARSRFHHNVVDMLRKAGGG
                     HARINVVRQIF"
     gene            3340..4770
                     /locus_tag="VF240_17830"
     CDS             3340..4770
                     /locus_tag="VF240_17830"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7177123.1"
                     /translation="MSTQIQNMNVDASALLLDSAGCGPEMELPISLMAAVGRLEDKRA
                     AVLVRHCFSALLNLLAELRDINASEAAEELDHAPEVITSVGDGAFVLIEHVEEASAKC
                     ESLKQDVEEALIGVCFALRHEVRRAIEIDLSAQDGDAQAGEYQGRVAQAYNLLYNCFQ
                     QCVVLLARSIDPKFDDRELFDDARLRLEQSVRLRCALVTLVDSVKKADGGRYPQSAVT
                     VIEEVEAFRRDHMRQLMRRDWETFDGFVNEIVATREPKDFTAAAKKFLAYLETLLGHV
                     QMRSVLKEQSAATAAAAARSSSRAKAPAANRFLSRSVLKPAAWAAAASVLLTCGLLFS
                     SRLSAEGGVASRVAPATHAAALVNAEPSEAAAPATNGAVKSREESRKAAPVKTVASAS
                     SNEGEGRLTVQVGAFGDMTSARSAVSTLAAKGVDARVERGSAAGQGVVFRVQVGRFKN
                     RGDAARFGGQLKAKGAVRTYLIAESE"
     gene            complement(4895..5938)
                     /locus_tag="VF240_17835"
     CDS             complement(4895..5938)
                     /locus_tag="VF240_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177124.1"
                     /translation="MEEKNQAPAAPKSEAPAAKSGPIGLDVGTSRIVAADGSGDFKAR
                     TQLNAFVAVPSSEMAENVLKHRGMVYERNCKNLYVYGNDSDFFASFLDTDARRPMHSG
                     LLNPQEEMSVQMMQRIIQLIVPRARRNEVLCFSVPGKGDGVNGNLVYHEAMLKSFLQS
                     LGYQAKGINEGQAVVFSELQEENFTGIGISFGGGMCNVSVSFMSIPMITFSVPKSGDY
                     IDRSVSEVLGERNSTRVRLHKEENLDLSRPPKDEMNRAMHIFYEDVLTTLMERMRAEF
                     QKSGQLPKIDRPMPIVLAGGTARPTGFLQKFETIMRSGGEFPVEISEVRMAKDPLTAT
                     ANGCYVAAMSESR"
     gene            complement(6124..7542)
                     /locus_tag="VF240_17840"
     CDS             complement(6124..7542)
                     /locus_tag="VF240_17840"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7177125.1"
                     /translation="MSPSLSIEQTLHSSRIVGRYAKFFERPEARLRFLNKTLSQETAT
                     RAKLDAALTNFEYVKKTRLYEQLLKLWLYQLVFRELYKQLPPGKRRVQLIDLRTRAPL
                     SARVFFTCYQFRHPLYVLGTVALMAALFGVYTGFAWSARRVSSLLAQRGTNGKAVAAD
                     STSNPVFAQVAASSLPDYQPERVWLVEEDEGYERFSNGARILREYETANHPRGYQVYA
                     PDGAPSGETGRQPVGIVYHTSESDLMPFNADHSESIEAHTRGLLEYVRKNSSYNYVID
                     RFGQIYRVVRDEDAANHAGNSVWAGAQGLFVGLNESFLGVCFETKTAAGVEERLTEAQ
                     LVSGRLLTQVLRSRHQIEDANCVTHGLVSVNPTSMLICFHHDWARGFPFEAMGLSDKY
                     AVPPASVTEFGFTYDAELLSKLGGALWPGVAAAEKTFNERAARENLRPDELRRRMREV
                     YRQRMDTQRGLRLEAAQAAAAD"
     gene            complement(7621..8526)
                     /locus_tag="VF240_17845"
     CDS             complement(7621..8526)
                     /locus_tag="VF240_17845"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7177126.1"
                     /translation="MEIKCPTCGLERTVDAVSGEHAEEQTCDSCAASYKALLAEIEVR
                     AAQRTTDADMAAHSEAPAPLSLTAPATEGESDVKSLSLETSARDEQEESDSDGYALGV
                     SLYRLPATGLAVGAVCLFAALFFAGLLRPADAAQSTSVIKPIAAAESAVKVGDESASS
                     PAVAELPPAAEMKADEPVAAEEASAEEIDEAQFEKEELAAEAGDEPEAAPAPEPAKST
                     GADGRFTVQVSSHNQPGEAQARASVLRASGFDARVAAVEIPKKGMWYRVQSGRFQTRE
                     QAALYEKSLRASGAAETTFVAESAN"
     gene            9166..11685
                     /locus_tag="VF240_17850"
     CDS             9166..11685
                     /locus_tag="VF240_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7177127.1"
                     /translation="MIALHRYRAARPLAGLIIAAGAAALVVSLAGLPSGQLDTRFLLL
                     SFLALSVGNTVFVKVPRVRAYVAASDLLVFVAALAYDARLAVPLAALCALAVSLRFSR
                     TAGGHLSGAMLGAAATYAASWAIGSLPAQTAGLQPTAELMLLSVFLLAFTRAAADSVP
                     TALGEAQETGTGAPRAYLKTLTWSFVAYFAAASAAGLLVTLAAWTGLDAVLVALATGG
                     VLHFTLRNRATAAARGAKVAGAAAEASHVALPSAGREVLLNAFEHAQIGMAVVSARGE
                     WVRVNRALCELLGRSEQELLSTDLQGVTHPDDLGPALAALKKLLKGKDTGMDTEVRHL
                     CGADGRHVWAHWSVGRFVAASNEEPHFIFQIQDIGERKVSEERLLHDAFHDPLTGLPN
                     RALFIDHVKMAISRARRRDDRPFAVLFIDLDRFKVINDSLGHLSGDDMLVGVARRLEG
                     CLREGDTVARVGGDEFTVLLDELQNAEEAIQIAERIQRDVSAAFQINGHEVFTTLSIG
                     IAHGAPEYADPEDILRDADTAMYRAKTLGKARHEVFDKAMHAFAVNLLQIETDLRKAL
                     DLKQFFIMYQPIVALDDFRLCGFEALVRWQHPERGLVSPMDFIPVAEETGQILQLGEW
                     TLLEASRQMNRWHRRFTSEFPFFISVNLSSKQFAQPNLIEQVDNILKRTSLNPRSLKL
                     EITESAVMDNIDKTTEMLKQLRQLGVQLSIDDFGTGYSSLSYLHRFPIDTLKIDRSFV
                     TRMVDNSENTEIVRTIVMLAQTLGMDVVAEGVETKEQLALLRQLGCEYGQGYYFSKPV
                     TAAEAERIISDIYERPREEGTPELVINDRVKSMRLVPTAQP"
     gene            11682..12050
                     /locus_tag="VF240_17855"
     CDS             11682..12050
                     /locus_tag="VF240_17855"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7177128.1"
                     /translation="MNSTGDRRRAERLRTSLQAYWEGVLTRRGGTITDISPTGCFILT
                     RDDVQPQELIRLDVHSPTGRSICLWGEVVYKVEEMGFALRFTGTEPAEQEMLKLLLDY
                     LRVVRESDQAAPELLTSNVG"
     gene            12040..12777
                     /locus_tag="VF240_17860"
     CDS             12040..12777
                     /locus_tag="VF240_17860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7177129.1"
                     /translation="MSASETQSRVFSGEGVLQTPLRVEWRAAGGEPRTETTRLLDADL
                     HGAAFALSEQVELGQMLKLTLPPVFESLRRAPDETQSETWALVWAVTPARASGGAVQR
                     PRHLTRVLFFGMDVPRNFVEGEGGPCLYVAEDDGSFRLHREDEGGDRAANRRRESRIH
                     LPYEVSAEVLDENGEVIASELLITENVSRRGAALRTTLDLRPYTRVRLTCAHFNFTVN
                     SVVRACRVGPDKIARMHVEFIDGQWPL"
     gene            complement(12774..13169)
                     /locus_tag="VF240_17865"
     CDS             complement(12774..13169)
                     /locus_tag="VF240_17865"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7177130.1"
                     /translation="MTDRRNDTRIHARAKVSVSCAFGPTEDTPRSANVTSLSIRGCFV
                     KTRAWAERGSEMFIRIWQKPERNWLTLRATVLYHMDKIGFGLRFDDVTPEQEEAIRAV
                     MEEALRGDSPAEEGYGDSVETQAGDGEAV"
     gene            13539..14165
                     /locus_tag="VF240_17870"
     CDS             13539..14165
                     /locus_tag="VF240_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177131.1"
                     /translation="MFSFGFVVAAMWVAAALTAAAAPAIQDRPGPPPPGNRDPFSEIR
                     ERQQREAQLRSVEMFGKAAPVDRRADEAAAAQVRQDFKRIQVLRNEIARHILSGKPLD
                     HKFIVEETAEVNRRAGRLKTFLAREPAEGNGKEQKRVGDMPESLLKDALVTLCKRIDS
                     FTENPMFKMPEVIDVKEFEMAEKDLDTIVLLSDAVRKGAEKAGKTAKK"
     gene            14264..15235
                     /locus_tag="VF240_17875"
     CDS             14264..15235
                     /locus_tag="VF240_17875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7177132.1"
                     /translation="MRYRRFGRTGWEVGEVGYGMWGMGGWTGSDDAESLLSLQRAVDL
                     GCNFFDTAWVYGQGRSETLLGELVGRNPHKRLYTATKVPPKNLEWPSRRHFTLDETFP
                     PAHIEEYVHKSLENARLESFDLVQFHVWEDAWLDDDRWAKKFDELRRRGLVKAVGISI
                     NRWEPWNGVRAVRSGLIDAVQVIYNVFDQNPEDELLPACVEMDVAVIARVPFDEGTLT
                     GTLTRESKWPEGDWRNTYFVPENLNQSVERADRLKPIVPDGTTMPEMALRFILSNPAV
                     STVIPGMRKTAHVESNIAASDAGPLPAELLAQLRAHRWERKPTEWSH"
     gene            15439..17340
                     /locus_tag="VF240_17880"
     CDS             15439..17340
                     /locus_tag="VF240_17880"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7177133.1"
                     /translation="MTKKFTRREFIESSVAAGALGLSFLSPAAAQRRRGAGAGRRGPR
                     RLSSHDREVRRMLSQMTLEEKVGQMVQAEITGLKDERDVERYFLGSLLSGGNGDPKTG
                     NSLKDWTDMYDSYQSRALKTRLAIPLVYGVDAVHGHNNVLGATVFPHNVGLGCTRSPS
                     LVERASRITSIEVRATGINWTFAPCVAVPRDERWGRTYEGFGETPELARTLGEAAVRG
                     LQGDDLADPLSILACAKHYAGDGGTTWGTGLPRPDTKERWGLDQGDTRLSESELREIH
                     MQGYVTAIRAGVGSIMPSYNSWNGVRCSGSRRLMTEILKEEMGFEGFLISDYNAIDAL
                     PGDYKSQIEQSTNAGMDMFMVPDKYRELYALLLELAKERRVSESRVDDAVTRILRVKF
                     AMGLMDKGRSPLADRSLHRSFGSAAHRKVARECVSASLVLLKNERRALPLSKRLARIH
                     VGGKSADDIGNQCGGWTISWQGKSGEVTPGGTTILKAIQNTVSRRTKVTHSADGSGAE
                     GADVGIVVIGETPYAEMVGDRENLTLAAEDVAAVRNMKRAGIPVVAVLLSGRPMIIEQ
                     ALGECDAFVAAWLPGTEGQGVADVLFGDYRPTGKLSFTWPRSMAQIPINAGDRRSDPL
                     FRYGFGLTF"
     gene            complement(17425..18798)
                     /locus_tag="VF240_17885"
     CDS             complement(17425..18798)
                     /locus_tag="VF240_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177134.1"
                     /translation="MEFGKEQKLEQLERVLHSRTLQNSESLKAFLRFVVEQTVDGRET
                     QLKEYIIATEVFGRNSDYDPRIDSVVRVQAGRLRTKLQEYYSTEGKGDPLVIDLPKGH
                     YHPVFNSAQPESVPDAAFNAHAAAHATGPNGHAATDTANGNAHDEMTHARRRGGMASS
                     LLLGAIIVTLGAAVLALYFSNRELRRQTVVADPSLNTEEFKAVWGPFLDDPEPPLLVL
                     SNPTVYRFVNEADPRSLIERAITLNSEQTRTLSAAPEFKGQWTGGESPRLIPSLGMYT
                     GMGEAIGLYRLTDLFRNAGKGVLLRQSRHVNAADLKYRNVVLLGSIYVNEWSRRLPLN
                     ENFAYTFSATIENREPLAGEERVYKPEFHPETGEMVVDYALITVKPNVSGEHAVMTLA
                     GIFSEGTEAAAEFVTTRNHLDLLGQRLRQLGAPGGGPPKYYQALLKVGVENGTPTTIK
                     LLSVRPL"
     gene            19386..22748
                     /locus_tag="VF240_17890"
     CDS             19386..22748
                     /locus_tag="VF240_17890"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7177135.1"
                     /translation="MKKKLGLHAMLFLLMMGLLGGVVSAQTSTVGSISGTVRDPQGAA
                     VPNAEVIILEETTGQTRTAKTDDDGSYSAQSLPVGRYSVSVAPQGFKKTVATGIEVHV
                     SDQVVVDINLEVGQVSETVTVTGAAQLVETESGRVSSLVTEKQVTELPLNGRNYAALV
                     TLVPGLSAPNEGGAFGTRGTGLDSHVDVSVNGNQSNANMWTVDGVNNMDVGSNATLLV
                     FPSIDSIAEFRVERNSFSAEYGQAQGAVINLVTKGGGNEFHGSLFEFFRNDKLNANDW
                     FSNRAGNFGPNDPAGEAGAPRIPRAPLRYNNFGGNFNGPIVKDRVFFFWSEEWRKERR
                     GTGPLTARVPTAQERVGDFSGPIRTGPIPNDPLTGLPFPGNRIPANRLSPAGLALMNI
                     FPLPNNPADPTGTNWISTPLQPVDTRQDLIRGDITITDKMNMMVRYINENWVHGQAAG
                     NFWGDTPFPTLSSDWEQPSHSFAIKLTNTLSSTAVNEFQFSKAGNDIFVSTNPASTAL
                     NESIASVFPTVFPRVEGTGFPTVGWGDGGYGNLWHQAPWENHQDLFIWKDDFSKVIGS
                     HNTKFGVLFSHNKKDEQPNGGSGLYTIQSFGARTGNAIAELLVRDLPLVEYTEFERQD
                     MTLGRWKDFEFYGTDTWKARRNLTFTLGLRYSMFPPAYADDDRITNWIPERYNGVNIN
                     TGFVRADEADAAGLPRALVNTYKGGLQPRVGLAWDIKGDGKTALRLGFGRYISRSNVI
                     ASLLRMSNNPPWNRVVDSGWNPGALTLADCPTCRSLDTINPALANAAVGVGGVNSVDP
                     NFKPPESWQWNLTVSHEVMKNTVAEVSYVGNHGLHLWRLINGSYNQVLPQFRTLVANG
                     ADANLYRRFGFTNGVTRDESTGDSNYHALQVWIDRRFSNNLAFQTAYTWSHAISNVPT
                     QSYISQTTDVFNYDLDRGDSDLDRRHSLVFNAVYALPRFQRWGRLASSILGDWQLNTI
                     ASFYSGTPLNIFSGRDTAGLGGAGSLRPNLVPGVDVYANGSDPLQILNPAAFAFPAPG
                     QFGSLGRGSIRGPGIKNVDFSMAKNWKIQERFGIQFRAEMFNVFNTVNFRAHNLSVAG
                     GGIENNLSNGGFGRANSTRGAREIQFGLKFNF"
     gene            22850..>23333
                     /locus_tag="VF240_17895"
     CDS             22850..>23333
                     /locus_tag="VF240_17895"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177136.1"
                     /translation="MYFASALLPLLLLLPHFQAPSDSFRKHHEAAEAHRRAGNTAAAE
                     AEYAAILAGAYPALGRIYTAQSNYREAVAALEAAVPYRPDSPEVLVGLAVAYFYAGQY
                     QKAVEPLNRVLAHDPRSAPARHMLGKTHFMMGEFAGAARELEAALALKPDDYDAAYTL
                     G"
BASE COUNT         4159 a         7667 c         7585 g         3922 t
ORIGIN      
        1 gcagcaggtg agcagcgccg tcgatgaggc cgctcagcaa gttcagcagg ccgcgcaaca
       61 ggcggcgcag caagtcggac aggccgtaga tcaagcggcg caggcggcgc aacgcgcggg
      121 ggagttggcg acgcaggccg cgcaggcgat tcagcaacag gcacaacggg ccgccgcgca
      181 ggccgccgac caaatcgcgg gcgcggccga ggccgccttc gacgccgtca acgacgtgat
      241 gtcgcaactg cccagcgccg acgacgctct gaatcaactc caaagggccg tcacagacgt
      301 gacgaacgac ttcccgcaga cgccgccatc gcccaccgcc gcgtgtccct tcgccgtcag
      361 ccccgagcgt ctgacggaac aggtgaacgc cgcgcgtaac agggccgagc agtcactcca
      421 acaagcgcaa gccgcgatca cgcagacggc ccaacgcgcc ttcgagcagg cgcagcaaca
      481 cgccggacag gtggccgacc agattcaaaa ctccgtgcaa gcggccgtcg cacaggtgca
      541 gcaggcgacc gagcaggccg tcgatcaggc acgccagacg acggagcaag cactccagca
      601 agtccaacaa accgcgcagg agggggcgca agcagcacag caagcgattc agcaggcgca
      661 gcagcaggtg agcagcgccg tcgatgaggc cgctcagcaa gttcagcagg cggcgcagca
      721 ggcggcgcaa caggtgcagc agcaggcaca gcaggccgtc caacaggcgc agcaggccgg
      781 cgcgatggcc gcacaggccg cgcaggacgc gcaacagcag gtggccgcca cgacccggca
      841 ggccgcgcag tcggtcgccg acacggctgc cgaatcactc tccagcgtga atcaggccat
      901 gtctcaactc ggcctctcgt aatcgctcga tgaccacgcc gccacgtcac gcgcgccgtc
      961 gtcgcgtgtg cgcgggccga gtttcaacta cagcctttcc tccacgccgc gggggcgcga
     1021 aatggcagaa cccatctcac tcgaaaagcc cgagttcaat ctcgtcgcct cgaccgaaga
     1081 gaccgaaagc tcggccgcgg caacggagac cacgcccgac acatcgttcc gcatcgccct
     1141 gctcggcgac ttcgcggggc gcgcgagccg cgccaacgcg accccatcag gccgccgcct
     1201 tttcgagatc gaccgggaca acttcgacac ggtgctccgc aatctcggcg tcgaactctc
     1261 cttgagcttc cccgccgccg ccgcgcccgt cgcactccgc ttcgcagagc ttgaagactt
     1321 tcaccccgac cgcatctacg aacgcctcga catcttccgc agtctgcgcg agacccgagg
     1381 gcgactggcc gacccttcga ccttcgacgc cgccgcggcc gaagtgcgag gctgggccga
     1441 ccccgccgcc gcgagtcccc gccgcgaacc gacggaagcg gccgacccgc aacgcgcgcc
     1501 cgcggccgac acgccggggc gcgacctcct cgaacagatg ctcggccgcg cggcaagtgc
     1561 cgacgcgccc tcccgcagtc cgtctgagcg gccggcggag ttgacggact tcggccgctt
     1621 cctcggcgac atcgtcaggc cgcatctggc gtcggttgac gaggggcaga aggcccgttt
     1681 gacggaggcg gttgacgagg ccgccgcgga gttgatgcgc gcgatcctgc atcacccgga
     1741 cttccagtcg ctcgaagcgg cgtggcgcgc cacccacttc ctcgtctcgc gcacggagac
     1801 cgacgagaat ctcaagctct tcctgctcga cttcacgcgc gaagagttga ccgagggtct
     1861 ccgcgctggc gaagacgcac gcggcggccg tctcggcgaa ttgctcgcag ggcactcggg
     1921 ccacacgcac gccgacgaac cgtgggcgtt gctcgtcggc aattacactt tcgaccgcac
     1981 gccggaagac gcggagctgc ttcaactcat ctccttcgtc gcgcgccgtg cgcgggcgcc
     2041 cttcgtcgcg gcggcgagca cacgcgccgc cggtctcgaa tccttcttcg ccgagccgtc
     2101 ggacgagtcg ctgccggagg gcgacgtggg agaagtcgcg ccggaggagt gggcctcgct
     2161 gcggcggctg cccgaagcac gttatctcgg tctggccggg ccgcgctttc tgctgcgcct
     2221 gccctacggc gcggagaccg acgcggccga gcggttcgac ttcgaggaga tgggcgccgg
     2281 cgatgagccg cgccacggag aatacctgtg gggcaatccc gctttcgcct gcgcgcatct
     2341 tctcgcggag gcgttcggcc gcgccggttg gggcatgcgc gcgggcgacg tgaaagagat
     2401 cgagggcctg cccctccacg tccacgaagt gcagggcgag acgcgcaaca agccctgcgc
     2461 ggaggtcgtg ctcaccgagc gcgccgccgc cgaagtgctg gagcggggcg tcatgccgct
     2521 gttgtcgttc aaaaacgccg accacgtcag actcgctcgc ttccagtcgc tcgcccaacc
     2581 cccgacgccg ctcgcgggac gctggtctta gaagatttga cgcacgacgt tgatacgagc
     2641 gtgacccccg ccggctttcc gcagcatgtc tacgacgttg tgatggaacc tcgaacgagc
     2701 cgccccgacg gccgtccaac cgtctctggc ctcggcgtcc acctcggccc cgcgctccag
     2761 tagcaggtga actatggccg cgtgtccgcc aaaggcggcg aacatcagcg ctgtgttccc
     2821 atccccatct ctggcgttca cgtcagcccc ccgcctcaga agcacctgaa ctgtggcgat
     2881 ctttccctcg aaagcggcca acatcagcgg cgtcctaccc acaacgtact catttacttg
     2941 agcttgcatt caaatcacct cgtccaaaag tcttcacccc ctcgtctttt gcaggataaa
     3001 agacgagact tgagtaattc ttgaactttg tttaacacaa ggagattatc cgacatttaa
     3061 aagcggctgc gtcgctcctg attcccaaca aaacgggcgc gttagttcaa tttggcagct
     3121 tcggtcattc ttttgaatcg acacttcaag ttgactcgcc ggcgcgggcg acctgtgccg
     3181 ccaagtcctt tctcatcaat gaccgtcaat ctcgctggat tggcattaac cttgccacga
     3241 ctgttgccgg caggggccgc tacgtgtcag gcacgcccga tttcgctcat cgatgaaaat
     3301 tgttctcagc caagtcggca cctacgccat cgcaaaaaaa tgtcaacaca aatccaaaac
     3361 atgaacgtcg atgcctccgc gcttctgctc gattcggccg ggtgcgggcc ggagatggag
     3421 ctgcccatat cgttgatggc agccgtcggg cggctcgaag acaagcgggc ggcggtgctg
     3481 gtgcgccact gtttctcggc gctcctcaat ctcctggcgg aactgcggga catcaacgcg
     3541 agcgaggcgg cggaagaact cgaccacgcg ccggaggtca tcacgtcggt cggggacggc
     3601 gctttcgtcc tgatcgaaca cgtcgaggaa gcgtcggcga agtgcgaaag cctcaaacag
     3661 gatgtggagg aggctttaat cggcgtgtgc ttcgcgctcc gacatgaagt gcgccgcgcc
     3721 atcgaaatcg acctctccgc gcaggacggc gacgcgcagg cgggcgagta tcagggcaga
     3781 gtcgcgcagg cctacaacct gctctacaac tgtttccagc agtgcgtagt tttgctggcc
     3841 cgctccatcg acccgaagtt cgacgaccgc gagttgttcg acgacgcccg gctgcggctc
     3901 gaacaatcgg tgcggctgcg gtgcgcgctc gtcacgctcg tcgattccgt taagaaggcg
     3961 gacggcgggc gttacccgca atcggccgtc acggtcatcg aggaggtcga ggccttccgg
     4021 cgcgaccaca tgcgccaact gatgcgtcgt gactgggaga ctttcgacgg tttcgtcaac
     4081 gagatcgtgg cgacgcgcga gccgaaggat ttcacggcgg ccgccaagaa gtttctggcc
     4141 tacctcgaaa ccctgctcgg gcacgtgcag atgcgctccg tgctgaagga gcagtcggcc
     4201 gccacggcgg cggcggcggc ccggtcgtcg tcacgcgcaa aggcgccggc ggcaaacaga
     4261 ttcctgtcgc gctcagtgtt gaagccggcc gcgtgggcgg ccgccgcgtc agtgttgctg
     4321 acgtgcggcc tcttgttctc ttcgcgcctg agcgcggaag gcggagtcgc gagccgcgtc
     4381 gccccggcga cacacgccgc cgcgttggtc aacgccgagc cgtcggaggc cgccgcgccg
     4441 gctacaaacg gcgccgtcaa gtcgcgggaa gagtcgcgga aggctgcgcc cgtgaagact
     4501 gtcgcgagcg cgtcgtcgaa tgagggtgag ggaagattaa cggtgcaggt gggcgcgttt
     4561 ggagacatga cttcggcccg ctcggcggtc tcgacgctgg cggcgaaggg cgttgacgcg
     4621 cgcgtcgagc gcgggagcgc cgcgggacag ggcgtcgtct tccgcgtcca ggtcggtcgc
     4681 tttaagaacc gtggggacgc cgcgcgcttt ggcggtcaac tgaaggcgaa gggcgccgtc
     4741 cgcacgtacc tgatcgcgga gagcgagtaa gccgccaaag aaatgcgagg cgcgcgcgtg
     4801 atgcttctgt gccggcgcgc cgcaaagtag aaatggctcg gacaacgcaa gatgtcgtcc
     4861 gagccatttt cgatttgccg aaccgttggg cgtcttacct gctctcagac atggcggcga
     4921 cgtagcagcc gttggccgtc gcggtgagcg ggtctttggc catgcggact tcggagattt
     4981 cgacggggaa ctcgccgccg gagcgcatga tggtctcgaa cttctggagg aagccggtgg
     5041 gccgggccgt gccgccggcc agcacgatgg gcatcgggcg gtcgatcttc ggcagttggc
     5101 ccgacttctg gaactcggcg cgcatgcgct ccatcagcgt cgtgagcacg tcctcgtaga
     5161 agatgtgcat ggcgcggttc atctcgtcct tcggcgggcg cgacagatcg aggttctcct
     5221 ccttgtggag acgcacgcgc gtggagttgc gctcgcccaa gacctcggag acgctgcggt
     5281 cgatgtagtc gccgctcttg ggcacgctga aggtgatcat cgggatcgac atgaaggaga
     5341 cggagacgtt gcacatgccg ccgccgaagg agatgccgat gccggtgaag ttctcctcct
     5401 gcaactcgga gaagacgacg gcctgcccct cgttgatgcc cttggcctga tagccgagcg
     5461 actggagaaa gctcttgagc atggcctcgt ggtagacgag gttgccgttg acgccgtcgc
     5521 ccttgccggg aacgctgaag cagaggactt cgttgcggcg ggcgcgcggg acgatgagct
     5581 ggatgatgcg ctgcatcatc tggacgctca tctcctcctg cggattcaaa agccccgagt
     5641 gcatcgggcg gcgcgcgtcc gtgtcgagga agctggcgaa gaagtcggag tcgttgccgt
     5701 agacgtagag gttcttgcag ttgcgctcgt aaaccatgcc cctgtgcttc aagacgtttt
     5761 cggccatctc cgagctgggg acggcgacga aggcgttgag ttgcgtgcgc gccttaaagt
     5821 cacccgagcc gtccgcggcg acgatgcggc tggtgccgac atcgaggccg atggggccgg
     5881 acttggcggc gggtgcttca gacttggggg cggcgggggc ctggttcttc tcttccatgt
     5941 tgtctcctta aatatgtgtg tggcgaatat tcgttcgcgg ggcgggtcaa ggggtttctg
     6001 gaattaaccc atcacctgtt ccagaaaatt agcaagcagc gtgccgcact acgcggcggc
     6061 cccggtagca ccgccggcgt gtgaggggat ggcgggccgt cggcggcgcg ggggccggat
     6121 gttttagtct gccgcggctg cctgagcagc ctccagacgc agcccgcgct gcgtatccat
     6181 gcgctggcga tagacttcgc gcatccgtcg gcgcaactcg tcgggcctca agttctcacg
     6241 cgcggccctt tcgttgaacg tcttctcggc cgcggccacg ccgggccaca gggcgccgcc
     6301 gagcttggag agcagttctg cgtcataggt aaagccaaat tctgtcacgc tggcgggcgg
     6361 cacggcgtac ttgtccgaga gacccatggc ctcgaagggg aagccgcgcg cccagtcgtg
     6421 gtggaagcag atgagcatgc tcgtggggtt gacggaaacc agcccgtgag tgacgcagtt
     6481 ggcgtcctcg atctggtggc ggctgcgaag aacctgcgtc agcagtcgcc ccgagacgag
     6541 ctgcgcctcc gtcagacgct cctcgacgcc ggccgccgtc ttcgtctcga agcagacgcc
     6601 gaggaagctt tcgttcaagc cgacgaacag tccttgcgcg ccggcccaga cggagttgcc
     6661 ggcgtggttg gccgcgtcct cgtcgcgcac gacgcgataa atctgcccga agcggtcgat
     6721 gacgtagttg taagagctgt tcttcctgac gtattcgagc aggccgcgcg tgtgggcctc
     6781 gatggactcg ctgtggtcgg cgttgaacgg catcaggtcg ctctccgacg tgtggtagac
     6841 gatgccgacg ggctggcggc cggtctcgcc ggaaggggcg ccgtcaggag cgtagacctg
     6901 atagccgcgc gggtggttcg ccgtctcgta ctcccgaagg atgcgcgcgc cgttgctgaa
     6961 tcgctcgtag ccttcgtcct cttcgacgag ccagacacgc tcgggctggt agtcgggcag
     7021 gctgctggcg gcgacctgcg cgaacacggg gtttgacgtg gaatcggcgg ccaccgcctt
     7081 gccgttcgtg ccgcgctgcg cgaggagcga gctgacgcgg cgcgctgacc aggcgaagcc
     7141 ggtatagacg ccgaacaggg cggccatcag cgccacggtg ccgaggacgt agagcgggtg
     7201 gcggaactgg tagcaggtga agaagacgcg ggcggagagg ggcgcgcgcg tcctcagatc
     7261 gataagctgg acgcggcgtt ttccgggcgg gagctgttta taaagttcgc ggaagacgag
     7321 ttgataaagc cagagcttta gcaactgttc gtagaggcgg gtctttttaa cgtactcgaa
     7381 gttggtgagc gccgcgtcga gcttggcgcg cgtcgccgtc tcctgcgaga gggtcttgtt
     7441 gagaaagcgc agacgggcct cggggcgctc gaaaaacttc gcgtaccggc ccacgatccg
     7501 tgaactgtgc agcgtctgct cgattgaaag actgggactc atgaacggct tccgcttgat
     7561 ctacagcgag tggcgtcttc cgcgacggcg cgacttcaag cgccggcaaa actgctcaca
     7621 tcaattcgct gactcggcga cgaaggtggt ctcggcggcg cccgacgcgc gcaggctctt
     7681 ctcgtagagc gcggcctgtt cgcgcgtctg gaagcggccc gactgcacgc gataccacat
     7741 cccttttttg gggatttcga ccgcggccac gcgcgcgtcg aaaccggagg cgcgcagtac
     7801 ggacgcccgc gcttgggcct cgcccggctg gttgtgcgag ctgacctgca cggtgaacct
     7861 gccatccgcg cccgtactct tcgcgggttc cggcgcaggc gcggcctccg gctcgtcgcc
     7921 tgcctcggcc gccaactctt ccttctcgaa ctgcgcctcg tcgatctctt ccgccgacgc
     7981 ttcctcggcc gccaccggct cgtcggcctt catctcggca gccggcggca actctgcgac
     8041 ggccggcgac gaagctgact cgtcgccgac tttaacggcg gactctgctg cggcgatagg
     8101 ctttatcacg gacgttgact gcgccgcgtc cgccggcctg agcaagccgg cgaagaagag
     8161 cgcggcgaag aggcagacgg cgcccacggc cagccccgta gccggcaagc gatagaggct
     8221 cacgcccagc gcatagccgt ccgagtccga ttcttcctgc tcgtcgcgcg cgctcgtctc
     8281 aagactgagt gatttgacat cggattcgcc ctcggttgcc ggcgccgtga gcgagagtgg
     8341 cgcgggcgct tcggagtgcg cggccatgtc ggcgtcggtc gtgcgctgcg cggccctgac
     8401 ttcgatctcc gcgaggagcg ccttgtagga agcggcgcag gagtcgcatg tctgttcctc
     8461 ggcgtgctcg cccgagacgg cgtcaaccgt cctttcgagg ccgcaggtgg ggcacttgat
     8521 ctccatcgaa ttttcctccg ggggctacac agattccgcg accgcgttgg ttgtcccaag
     8581 tccgccgggc gcggcgggga aagcttgtct gttcatcgac cggccgcacg tcgcgcggcc
     8641 cctcgcctgt ctggcgtcgg cgaggcatga agtttcccgc atctggaata aacaaatcac
     8701 atgccagcgc gtgttccaaa ttcaactctc gttgagactt tccccggccc gcaggggcga
     8761 cgccaaatcg cccgccgtgg ggcgggtttc cctctctcgc gttgaacgtc tcgcgtgagg
     8821 tggggacgac gaacggccgg gcgcgtgaca aagtttgtca tcttcacctg actttttttg
     8881 ctatttgaac gacgggcaag caggctgacc ggcagtttga ggccgagcgc gagcggcagg
     8941 tggaggaaac cgcgggagac aggctaaacg attgtcggca cacatattgc tggagtcacg
     9001 tcgcgcttgc gcgggcgtgg aagtcaccga accgtgcgaa caatgccagg cgtgcggcaa
     9061 taagtttgaa agaaagtacg tttacatcct tcgccttccc tgccgccctc cctgccgccc
     9121 caaagcttca gccacgttga aagattcact accagaatca tccacatgat tgctttgcac
     9181 aggtatcgcg cggccagacc gctcgcaggt ttaatcatcg ccgccggcgc ggcggcgctt
     9241 gtcgtctcgc tcgcggggct gccatcgggg cagttggaca cgagattcct gctgctgagc
     9301 ttcctcgcgc tgagcgtcgg caacacggtc ttcgtcaagg tgccgcgtgt cagggcttac
     9361 gtggccgcgt ccgacctgct cgtgttcgtg gcggcgctgg cctacgacgc acgtctcgcc
     9421 gtcccgctcg ccgcgctgtg cgcgctggcc gtgtcgctgc gcttcagccg cacagccggc
     9481 ggccacctct cgggcgcgat gctcggcgcc gccgcgacct acgccgcctc ctgggccatc
     9541 gggtcgctcc ccgcacagac cgccggcctt cagccgacgg cggaactcat gctcctctcc
     9601 gtcttcctcc tcgccttcac gcgcgcggcc gccgactccg tgcccacggc gctcggcgaa
     9661 gcgcaagaga cggggacggg cgctccgcgg gcttacctga agacgctcac gtggtcgttc
     9721 gtcgcttact tcgcggcggc gtcggcggcg ggcctgctgg tgacactggc cgcgtggacg
     9781 ggcctcgacg ccgtcctcgt cgcgctcgca accggcggcg tcctacactt caccctccgc
     9841 aacagggcga ccgccgctgc gcgcggcgcg aaggtcgcgg gcgccgcggc ggaggcgtcg
     9901 cacgtcgcgt tgccgtcggc gggcagggaa gttttgctca acgcgttcga gcacgcgcag
     9961 atcgggatgg ccgtggtctc ggcgcgcggc gagtgggtca gagtcaaccg cgcgctgtgc
    10021 gagctgctcg ggcgctccga gcaggagctt ctgagcacgg acttgcaggg cgtgacacac
    10081 ccggacgact tggggcctgc gctcgccgcg ctgaaaaaac ttttgaaggg caaagacacg
    10141 gggatggaca ccgaggtgcg ccacctgtgc ggcgccgacg gccgtcacgt ctgggcgcac
    10201 tggagcgtcg gtcgcttcgt cgccgcgtcc aacgaggagc cgcacttcat ctttcagatt
    10261 caggacatcg gcgagcgtaa agtgtcggaa gagcgcctgt tgcacgacgc cttccacgac
    10321 ccgctcacgg gcctcccgaa ccgcgcccta ttcatcgatc acgtgaagat ggccatctcg
    10381 cgcgcccgac gccgcgacga ccgccccttc gccgtcctct tcatcgacct cgaccgcttc
    10441 aaggtcatca acgattctct gggccacctc tccggcgacg acatgctcgt cggcgtggcc
    10501 cgccgcctcg aaggatgtct gcgcgagggc gacacggtgg cgcgcgtcgg cggcgacgag
    10561 ttcaccgtgc tgctcgacga attacaaaac gcggaggagg ccatccagat tgccgagcgc
    10621 atccagcgcg acgtgtcggc ggccttccag atcaacggcc acgaggtctt caccacgctg
    10681 agcatcggca tcgcccacgg cgcgcccgag tacgccgacc ccgaagacat cctgcgcgac
    10741 gccgacacgg ccatgtaccg cgcgaagact ttgggcaaag cccgccacga agtcttcgac
    10801 aaggccatgc acgccttcgc cgtcaatctt ctccagatcg agaccgacct gcggaaggcg
    10861 ctcgacctca agcagttctt catcatgtac cagcccatcg tcgcgcttga cgactttcgc
    10921 ctctgcggct tcgaggcgct ggttcgctgg cagcaccccg agcgcggtct ggtctcgccg
    10981 atggacttca tccccgtcgc cgaggagacc gggcagattc tccaactcgg cgagtggacg
    11041 ctcctcgaag cctcgcggca gatgaatcgc tggcaccgcc gcttcacctc cgagttcccg
    11101 ttcttcatca gcgtcaattt gtcgagcaag cagttcgcgc agccgaacct catcgagcag
    11161 gtggacaaca tcctgaagcg gacgagcctc aacccgcgct cgctgaagct ggagatcacc
    11221 gagtcggccg tgatggacaa catcgacaag acgaccgaga tgctcaagca gctccgccag
    11281 ctcggcgtgc agctctccat cgacgacttc ggcaccggct actccagtct ctcttacctc
    11341 caccgcttcc ccatcgacac cctcaagatc gaccgctcct tcgtcacccg catggtcgat
    11401 aactcggaga acacggagat cgtccgcacc atcgtcatgc tcgcgcagac gctcggcatg
    11461 gacgtggtgg ccgagggcgt cgagacaaaa gaacaactcg cgctgctgcg gcagctcggc
    11521 tgcgagtacg gccaagggta ctacttctcc aagccggtca ccgccgccga ggccgagcgc
    11581 atcatctccg acatctacga gcggccccgc gaggagggca cgcccgaact cgtcattaac
    11641 gaccgggtca agtccatgcg actggtcccg acggcacagc catgaattca acaggggaca
    11701 gaagaagggc cgagcgcctg cggacgagct tgcaggctta ctgggagggc gtcctcacgc
    11761 gccgcggcgg caccatcacg gacatcagcc ccaccgggtg cttcatcctg acgcgcgacg
    11821 acgtgcagcc gcaggaactc atccgcctcg acgtacactc gccgacgggc cgctccatct
    11881 gcctgtgggg cgaggtcgtc tacaaggtgg aggagatggg cttcgccctg cgcttcacgg
    11941 gcaccgagcc ggcggagcag gagatgttga agctattgtt ggactacctg agagtggtgc
    12001 gcgagtccga ccaggccgcg ccggagttgt tgacgagtaa tgtcggctag cgaaacacag
    12061 agtcgagtct tcagcggcga gggagtgttg cagacgccgc tccgcgtcga gtggcgggcc
    12121 gccggaggcg agccgcgcac ggagacgacg cgtctgctcg acgccgacct ccacggcgcg
    12181 gccttcgctc tctccgagca ggtcgagctg gggcagatgt tgaagctgac gctgccgccc
    12241 gtcttcgaga gcctgcgccg cgcgcccgac gagacgcaga gtgagacgtg ggcgctggtg
    12301 tgggccgtca ccccggcgcg cgcgtcgggc ggcgccgtgc agcggccgcg tcatctgacg
    12361 cgcgtcctgt tcttcgggat ggacgtgccg cgcaacttcg tggagggtga gggtgggccg
    12421 tgcctgtacg tggccgagga cgacggcagc ttccggctcc accgtgaaga cgaggggggc
    12481 gaccgggcgg cgaatcgccg gcgcgagtcg cgcatccacc tgccctacga ggtgtcggcg
    12541 gaggtgttgg acgagaacgg cgaagtgatc gcgagcgagc tgttaatcac ggagaacgtg
    12601 agccggcgcg gggcggcgct gcgcacgacg ctcgacctgc ggccgtacac gcgcgtccgt
    12661 ctgacctgcg cgcattttaa tttcactgtc aactcggtcg tccgcgcctg tcgcgtcggc
    12721 cctgataaga tcgcgcggat gcacgtcgaa ttcatcgacg ggcagtggcc gctctaaacg
    12781 gcctcgccgt ctcccgcctg agtttccaca ctgtcgccgt agccttcctc ggccggcgag
    12841 tcgccgcgca acgcctcctc catcaccgcc cggatcgcct cctcttgttc gggcgtcacg
    12901 tcgtcgaacc tgagtccgaa gccaatcttg tccatgtggt agagcaccgt cgcccgcagg
    12961 gtcagccagt tccgctccgg cttctgccag attctgataa acatctccga cccgcgctcg
    13021 gcccacgcac gcgtcttcac gaaacagccc ctgatgctca ggctcgtgac gttggcgcta
    13081 cggggcgtgt cctcggtcgg cccgaaggcg caggagacgg agactttggc gcgggcgtgg
    13141 atacgtgtgt cgtttcgtcg gtcggtcatg tcgtgtgtgt tgggcagtct cgtctggtaa
    13201 ggccgattag tgcaacggcg gcgacggcgc cgcggaagtt ccgcggcggc cgaaaagttt
    13261 tgtcggggcg aggaaatagt agcagcaata atgaaacata caacggcgcg cgcgggcaag
    13321 tggcgcaccg agcgggcgcg ccggtcttca gataacaccg gcgacaccgg cggacggatc
    13381 aggccggtcg aaacgctcgg ggttcgcggg cgcgccgccc ggatgttgtc cgcgactgaa
    13441 gcgcgggatt atactgcctg ctcgaagacg acagaatcct gctcgaagac gatagaataa
    13501 ggagagcggc catgaggagt cggcagtcgc ccgcgtcaat gttctcgttc ggattcgtgg
    13561 tggcggcgat gtgggtcgcg gccgcgctca cggccgccgc ggctccggcc atacaggaca
    13621 ggcccggccc gccgccgccc ggcaaccgcg acccgttctc cgaaatccgc gagcggcagc
    13681 agcgcgaggc gcaactgcgt agcgtcgaga tgttcggcaa ggcggcgccc gtagaccggc
    13741 gcgccgacga ggccgccgcc gctcaggtga ggcaggactt caagcgcata caggttctcc
    13801 gcaacgagat cgcgcgccac atcctttcgg gcaagccgct cgaccacaag ttcatcgtcg
    13861 aggagacggc ggaggtcaac cggcgcgccg gtcgtctgaa gacgttcctg gcgcgggagc
    13921 ctgcggaggg caacggcaaa gagcagaagc gtgtgggcga tatgcccgag agcttgttga
    13981 aggacgcgct cgtgactttg tgcaagcgca tcgacagctt cacggagaat cccatgttca
    14041 agatgccgga agtgatcgat gtcaaagagt tcgagatggc cgagaaagat ttagacacca
    14101 tcgttctgct cagcgacgcc gtcaggaagg gcgctgagaa ggctggcaag acggcgaaga
    14161 agtaaggcgg cgctcacacg tcggccgaag atgggcgcgg ccgtcgagtg ttgatgcatc
    14221 cgcgtcttca gacgggggtc gagttttcgc ggggagggca gcgttgaggt atcgtcgttt
    14281 cgggcgcacg ggttgggagg tcggcgaggt cggctacggc atgtggggca tgggaggttg
    14341 gacggggtcg gacgacgcgg agtcgctgct ctcgctccag cgcgccgtcg atctcggctg
    14401 caacttcttc gacaccgcct gggtctacgg acagggacgc agcgaaactc tgctcggcga
    14461 actcgtcggc cgcaacccgc acaagcgact ctacacggcc accaaagttc ccccgaagaa
    14521 tcttgagtgg cccagccgcc gccacttcac gctcgacgaa actttcccgc ccgcacacat
    14581 cgaagagtac gtccacaaga gtctggagaa cgcgcggctg gagagtttcg acctcgtgca
    14641 gttccacgtg tgggaagacg catggctgga cgacgaccgc tgggcgaaga agttcgacga
    14701 gctgcgccgg cggggtttgg tcaaagccgt cggcatcagc atcaatcgtt gggagccttg
    14761 gaacggcgtg cgcgccgtgc gtagcgggct gatcgacgcc gtgcaggtca tctacaacgt
    14821 cttcgaccag aacccggagg acgaactcct ccccgcctgc gtcgagatgg acgtggccgt
    14881 catcgcgcgc gtgcccttcg acgagggaac gctcacgggc acgctcaccc gtgagagcaa
    14941 gtggccggaa ggcgactggc gcaacaccta cttcgtgcct gagaacctga accagagcgt
    15001 cgagcgcgcc gaccgcctca agccaatcgt ccccgacggc acgacgatgc ccgagatggc
    15061 cctgcgcttc atcctcagca acccggccgt cagcaccgtc atccccggca tgcgtaagac
    15121 cgcccacgtc gaatcgaaca tcgcggcgag cgacgccggc ccgctccccg cggaactgct
    15181 cgcgcagctt cgcgcacacc gctgggagcg caagccgacc gagtggtcgc actgacgacg
    15241 gaagaatttc cgcacccgcc tacacgcaaa aaactccgag cacgcgcccg cgcgtctacc
    15301 gaaatccttc ctgcttcgtg ggtatctcac gacgcagtta accgtgtcta acttacattc
    15361 cgactcgtgt atgagtgagt ctataatcat ctacacacgc tcgacgatca cttaacccga
    15421 cgaaccactc tcgaccttat gacgaaaaag ttcacccgcc gcgagttcat cgagtcttcc
    15481 gtcgccgccg gcgccctcgg cctctcgttc ctttcgcccg ccgccgccca gaggagaagg
    15541 ggcgccggcg ctggccgacg cgggccgcgc cggctctctt cccacgaccg agaagtccgg
    15601 cggatgctct cgcagatgac tctggaggag aaggtcgggc agatggttca ggccgagatc
    15661 accggcctca aggacgagcg cgatgtggag cgttacttcc tcggctcact cctcagcggc
    15721 ggcaacggcg acccgaagac cggcaacagc ctcaaggact ggacggacat gtacgactcc
    15781 taccagtcgc gcgcgctcaa gacgcgcctc gccatcccgc tggtctacgg cgtcgatgcc
    15841 gtgcacggcc acaacaacgt cctcggcgcg actgtgttcc cgcacaacgt cggcctcggc
    15901 tgcacgcgca gccccagcct cgtcgagcgc gcctcgcgca tcacctcaat cgaagtccgc
    15961 gccacgggca tcaactggac gttcgccccc tgcgtcgccg tcccgcgcga cgagcgctgg
    16021 ggccgcacct atgaaggctt cggcgagacg cccgaactcg cacgcacgct gggcgaggcc
    16081 gccgtccgag ggcttcaggg cgacgacctc gccgacccgc tgagcatcct ggcctgcgcc
    16141 aagcactacg cgggcgacgg cggcaccacg tgggggacag ggctgccgcg gcccgatacc
    16201 aaagagcgtt gggggctgga tcagggcgac acgcggctga gcgaaagtga gttgcgagaa
    16261 attcacatgc agggctacgt cacggccatc agggcaggcg tcggttcgat catgccctcc
    16321 tacaacagtt ggaatggggt gaggtgttcg ggcagtcgcc gtctgatgac tgagattctg
    16381 aaagaggaga tgggcttcga gggcttcctg atttccgact acaacgccat cgacgcgctg
    16441 cccggtgatt acaagagcca gatcgagcag tccacgaacg ccgggatgga tatgttcatg
    16501 gtgcctgaca agtaccggga gctgtacgcg ctgctgctgg agctggcgaa ggagcggagg
    16561 gtgagcgagt cgcgagtgga cgacgccgtg acgcgaatcc tgcgggtcaa gttcgcgatg
    16621 ggactgatgg acaagggccg gtcgccgctg gccgacagaa gtctacatcg gagcttcggc
    16681 tcggccgcgc accgcaaggt ggcccgcgag tgcgtgagcg cgtcgctggt gctgctcaag
    16741 aacgagcggc gggcgctacc gctgtcgaag cgtctggcgc gcatccacgt cggggggaag
    16801 agcgctgacg acatcggcaa tcagtgcggc ggctggacga tcagttggca ggggaagagc
    16861 ggggaggtga cgccgggcgg cacgaccatc ctcaaagcca ttcagaacac cgtctcaaga
    16921 aggactaagg tgactcactc cgcggacggc agcggcgccg agggggccga cgtagggata
    16981 gtcgtcatcg gcgagacgcc ctacgccgag atggtcggcg accgggaaaa cctgacgctc
    17041 gcggccgaag acgtggcggc cgtgcgtaac atgaagcgag cgggaatccc cgtcgtcgcc
    17101 gtgctgctct ctggacggcc gatgatcatc gaacaggcgc tcggcgaatg cgacgcgttc
    17161 gttgctgcgt ggctgccggg gacggaaggg cagggcgtgg ccgacgtgct ctttggcgac
    17221 tatcgcccga cgggaaaact gtcgttcacc tggccgcgct cgatggcgca aatccccatc
    17281 aacgccggag accgccgctc cgacccactc ttcagatacg ggttcggcct gacgttttga
    17341 gggatgaggg cggagggatg agggatgaag aggaactgct caacttgagc cttctccttc
    17401 atccctcacc cttcatccct gtctttacag cgggcgcact gagaggagct tgatcgtggt
    17461 gggcgtgccg ttctcgaccc cgaccttgag gagcgcctga tagtacttcg gcggcccgcc
    17521 tccgggcgcg ccgagttggc gcaggcgctg gccgaggagg tcgaggtggt tgcgagtggt
    17581 gacgaactcg gccgccgcct ccgtcccctc gctgaagatg cccgccagcg tcatcaccgc
    17641 gtgctcgccc gagacgttcg gcttgacggt gatgagcgcg taatcgacca ccatctcgcc
    17701 cgtctccggg tggaactccg gcttgtagac gcgctcctcc ccggcgagcg gctcgcggtt
    17761 ctcgatggtc gccgagaagg tgtaggcgaa gttctcgttc aggggcagac ggcgcgacca
    17821 ctcgttgacg tagatgctgc cgagcaggac gacgttgcgg tacttgaggt cggcggcgtt
    17881 gacgtggcgg ctctgcctga gcaggacgcc cttgcccgcg ttgcggaaga ggtctgtgag
    17941 gcggtagagg ccgatggcct cgcccatgcc cgtgtacatg cctaacgagg ggatgagccg
    18001 cggcgactcg ccgcccgtcc actgtccctt gaactcgggc gcggccgaga gcgtgcgcgt
    18061 ctgttcagag ttgagagtga tggcgcgctc gatgagcgac cgcggatcgg cctcgttcac
    18121 gaagcggtag acggtcgggt tgctcaggac gagcagcggc ggctccgggt cgtcgaggaa
    18181 cggcccccag acggccttga actcttcggt gttaagcgag gggtcggcga ctactgtctg
    18241 ccgccgcaac tcccggttcg agaagtagag cgccagcacg gcggcgccga gcgtgacgat
    18301 gatcgcgccc agcagcagag aagacgccat gccgccgcgg cggcgcgcgt gcgtcatctc
    18361 gtcgtgtgca ttaccgttcg cggtatccgt cgccgcgtga ccgttggggc cggtcgcgtg
    18421 cgccgcagcg tgcgcgttga aagcggcgtc gggcacggac tcgggttggg cgctgttgaa
    18481 gacggggtgg tagtggccct tggggaggtc gatgacgagg gggtcgcctt tcccctcggt
    18541 cgaataatac tcctgaagct tcgttcgcag tctcccagcc tggacgcgca ccacggagtc
    18601 gatgcgcggg tcgtagtcgc tgttgcggcc gaagacctcc gtggcgatga tatattcttt
    18661 gagctgtgtc tcgcggccgt ctacagtctg ctccacgaca aaacggagga aggccttgag
    18721 gctctccgag ttttgcagcg tgcggctatg gagaactcgc tccaactgct ccagcttttg
    18781 ttccttacca aattccatag atcaaggtca caacctgaga gtccacgacc ggatgttgaa
    18841 gatgggacga cgctagtgtc accccggaga aggttcgatt tgacaacggt tgttcggtgc
    18901 cccaactaat ggctggcagg tgccgggtct cggccgtttc ggattataag cccgtgacca
    18961 tcttttcaac aaccgggcgg gaggccgcaa gactcgcccg ccgccggcac acgggacgaa
    19021 ggaaaacact cgattattcg cgatgtaacg agttacacgg ccgatgtaac gcgtgcgttc
    19081 ctcatctgta tgtgggctga actcgtgtgt cttaccgtat catacggatg gtaactttta
    19141 tgatgttaat tgtgggtata aagtgtactt aagtcctagt ctgcccggta tcagccgggc
    19201 gttgagctta tcaatcgatt gccgcggtga caatggccct ttcacttcga agggcgtgcg
    19261 aggccgacaa agcccgaatc tgcggtcagc gtttaagatt tcagactcga atacttcgca
    19321 ggattgaccg gcgcggaagt gccgcacgcg ggcgcggccg ccacacgtta caggagagcc
    19381 aaaccatgaa aaagaagctc ggtcttcacg caatgctgtt cctgctgatg atgggattgt
    19441 tgggcggcgt cgtgagcgcc cagacgagca ccgtcggaag catcagcggc accgtgcgcg
    19501 acccgcaggg cgccgccgtc cccaacgccg aggtcatcat tctcgaagag acgacggggc
    19561 agacgcgcac agccaagacc gacgacgatg gctcctactc ggcccagagt ctccccgtgg
    19621 gccgctacag cgtgagcgtc gctccgcagg gattcaagaa gacggtcgcc acagggatcg
    19681 aggtgcacgt cagcgatcag gtggtggtcg atatcaatct cgaagtcggg caggtcagcg
    19741 agacggtgac ggtgaccggc gcggcgcagc tcgtcgagac ggagagcggg cgcgtcagca
    19801 gcctcgtcac ggagaagcag gtcacggagc tgccgctcaa cggacgcaac tacgcggcgc
    19861 tggtgacgct ggtgccgggc ctgtcggctc cgaacgaggg cggcgccttc ggcacccgcg
    19921 gcaccgggct tgactcgcac gtggacgtgt ctgtgaacgg caaccagtcg aacgcgaaca
    19981 tgtggacggt ggacggcgtc aacaacatgg atgtcggctc gaacgccacg ctgctcgtct
    20041 tcccgtccat cgactccatc gccgagttcc gcgtcgagcg caatagcttc agcgccgagt
    20101 atggacaggc gcagggggcc gtcatcaacc tcgtcacgaa gggcggcggc aacgagttcc
    20161 acggctcgct cttcgagttc ttccgcaacg acaagctcaa cgccaacgac tggttctcga
    20221 accgcgcggg caacttcggc ccgaacgacc ctgccggcga ggccggggca ccgcgcatcc
    20281 cgcgcgcgcc gctgcgctac aacaacttcg gcggcaactt caacggcccc atcgtcaagg
    20341 atcgggtctt cttcttctgg tcggaggagt ggcgcaagga gcggcgcggc acggggccgc
    20401 ttaccgcccg cgtccccacc gcgcaggaga gagtgggaga cttcagcggc cccatccgta
    20461 ccggcccgat accgaatgac ccgctcaccg gtctgccgtt tcccggcaac cgcatcccgg
    20521 cgaaccgtct gagtcccgcg ggcctcgcgc tgatgaacat cttcccgctc cccaacaacc
    20581 cggccgaccc gaccggaacc aactggattt cgacgccgct ccagcccgtg gacacgcgcc
    20641 aggacctcat tcgcggcgac atcaccatca ccgacaagat gaacatgatg gtgcgctaca
    20701 tcaacgagaa ctgggttcac gggcaggcgg cgggcaactt ctggggcgac accccgttcc
    20761 cgacgctctc ctcggactgg gagcagccca gccacagctt cgccatcaag ctgacgaaca
    20821 cgctgagctc gaccgccgtc aacgagttcc agttctcgaa ggcgggcaac gacatcttcg
    20881 tctcgaccaa cccggccagc acggctctca acgagagcat cgcctccgtg ttccccaccg
    20941 tcttcccgcg cgtcgagggc acgggcttcc cgaccgtcgg ctggggcgat ggcggctacg
    21001 ggaacctctg gcatcaagcg ccgtgggaga accatcaaga cctcttcatc tggaaggacg
    21061 acttctccaa agtcatcggc tcacacaaca ccaagttcgg cgtcctcttc agccacaaca
    21121 agaaggacga gcagccgaac ggcggcagcg gcctctacac catccagtcg ttcggcgcgc
    21181 gcacgggcaa cgctatcgcc gagttgctcg tcagagacct gccgctcgtc gagtacaccg
    21241 agttcgagcg gcaggacatg acgctcggcc gctggaaaga ctttgaattt tacgggaccg
    21301 acacctggaa ggcgcgacgc aacctgacgt tcacgttggg tctgcgctac tcgatgttcc
    21361 cgccggccta cgccgacgac gaccgcatca ccaactggat acccgagcgc tacaacggcg
    21421 tcaacataaa caccggcttc gtccgcgccg atgaggccga cgcggccggg ctgccccgcg
    21481 cgctcgtcaa cacctacaag ggcggcctcc agccgcgcgt cggtctcgcg tgggacatca
    21541 agggcgacgg caagaccgcg ctccgcctcg gcttcggccg ctacatcagc cgctcgaacg
    21601 tcatcgcgtc gctgctgcgg atgtccaaca atccgccttg gaacagggtg gtcgattcgg
    21661 gctggaaccc gggggcgctc accctcgccg actgcccgac ctgccgcagc ctcgacacca
    21721 tcaacccagc gctggcaaac gccgcggttg gcgtcggcgg cgtcaactcc gtcgatccga
    21781 acttcaagcc gcccgagagc tggcagtgga acctgaccgt ctcccacgag gtcatgaaga
    21841 acaccgtcgc cgaggtctcc tacgtcggca accacggcct ccatctctgg cggctcatca
    21901 acggcagtta caatcaggtg ctgccgcagt tccgcacgct ggtcgcgaac ggcgccgacg
    21961 cgaacctgta tcgacgcttc ggcttcacca acggcgtcac gcgcgacgag tcaacgggcg
    22021 actccaacta ccacgcactt caggtctgga tcgaccggcg cttctctaac aatctcgcct
    22081 tccagaccgc ctacacgtgg tcgcacgcca tcagcaacgt gcccacccag tcgtacatca
    22141 gtcagacgac cgatgtcttc aactacgacc tcgaccgggg cgactccgac ctcgaccgcc
    22201 ggcactccct cgtcttcaac gccgtctacg ccctgccaag gttccaacgc tggggccgtc
    22261 tggcgagcag catcctcggc gactggcagc tcaacaccat cgcctccttc tactcgggga
    22321 cgccgctcaa catcttctcc ggcagagaca cggccggtct cggcggcgcc ggcagcctgc
    22381 gccccaatct cgtccccggc gtagacgtct acgccaacgg cagcgatccg ctccagatcc
    22441 tcaacccggc ggccttcgcc ttccccgcgc cggggcagtt cggctcactc gggcgcggct
    22501 ccatccgcgg gccgggcatc aagaacgttg acttctcgat ggcgaagaac tggaagatac
    22561 aggagcgatt cggtattcag ttccgcgccg agatgttcaa tgtcttcaac accgtcaact
    22621 tccgcgcgca caacttgagc gtcgccggcg gcggcatcga gaacaacctc agcaacggag
    22681 gcttcggccg ggccaactcc acacgcggcg cgcgcgaaat tcagttcggt ttgaaattca
    22741 acttctgata tagttcggca aggccgagac gcggacggag ggctgaagga ttaacttcag
    22801 ccctccgtcc gggtctttgc cgagtccacc ccaagcagtc aaggtagcaa tgtatttcgc
    22861 ctcggccctc ctgccgctcc tgctcctgct cccacacttc caagccccga gcgattcatt
    22921 cagaaaacat cacgaggcgg ccgaggcgca ccggcgcgcc ggcaatacgg cggccgccga
    22981 ggccgagtac gcggccatcc tcgccggcgc gtaccccgcg ctcgggagga tttacaccgc
    23041 gcagtcaaac taccgcgagg cggtcgcggc ccttgaggcg gccgtccctt accggcccga
    23101 ctctcccgaa gtgctcgtcg ggctggccgt cgcctacttt tacgccgggc agtaccagaa
    23161 ggcggtcgag cctttgaacc gcgtcctcgc acacgacccg cgtagcgccc ccgcgcgcca
    23221 catgctcggg aagacacact ttatgatggg cgagtttgcg ggcgccgcgc gcgaactcga
    23281 agcggcattg gcgctgaagc cggatgatta cgacgcggcc tacacgctcg gtc
//
LOCUS       DASVES010000436         4352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_81838, whole genome shotgun sequence.
ACCESSION   DASVES010000436 DASVES010000000
VERSION     DASVES010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81838"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1146)
                     /locus_tag="VF240_17900"
     CDS             complement(76..1146)
                     /locus_tag="VF240_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7177137.1"
                     /translation="MKFDGTVFLTGFPGFIAGRLVRRLAEDGARFLLLVQPALAERAR
                     AEVAGIVEETGTPARNFHILEGDITRPQLGLSPSDLERARSETTAVFHLAAVYDLAVA
                     REAAVLVNLEGTRNVNEFARTLPHLRRYHYVSTCYVAGKRTGLIREDELRHEAGFRNY
                     YEETKYLAEVEVEALKRELPVTVHRPAVVCGDSRTGETAKYDGVYYLIQYLRIRPRVL
                     ALANIGNDEVRLNIVPVDFVVEAMAALAREERAAGATVQLADPAPLTTRELFDIIAGV
                     LTGHGSRLIVPAPLVRASLTLPLSDTLTKLPHSGVPYFFIKQTYDTARAAELLEPHGV
                     RCPSFTTYAAALVRFVEQHPKL"
     gene            complement(1149..2681)
                     /locus_tag="VF240_17905"
     CDS             complement(1149..2681)
                     /locus_tag="VF240_17905"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX7177138.1"
                     /translation="MNKPNSLSIRIATEIPPENPPGQAAAVCVFADEPRAPGDAARLD
                     EVLGRVCQQAVRDGEFDGEAETALMLRVRGAGGSDGVRRVLLVGLGARAHFDEAVLRR
                     AAGVAAREARAARVKDLHFVLPPGVEATAARASARAVVEGALLGLYDNDFYQQQNGDE
                     PAPALERLVIVAPHGGGVELSAEVERARIVAESVNWARTLADEPGLSLPPREFARRAA
                     VMAAEVGLRIETLGAEEIRARGMGALLGVAQGSEEEPALIVLRYEPEGARADDELWAF
                     VGKGITFDTGGISLKRGPGMEEMKSDMAGGAAALGALRALAQLKPRRRVVGIVPAAEN
                     MPSGTAIKPGDIVRTLAGHTIEVVDTDAEGRLVLVDGIAYAKQLGATRIVDLATLTGS
                     IIVALGDHRTGLFSNDDAWAGQLRAASERAGEPVWRMPVSDDYKKRIESDIADFKNYG
                     GRPDATGAALLLSKFAGDTPWVHLDIAGTAWYDEARPYAPQGASGAGVRTLVELVCGR
                     EA"
     gene            2892..>4352
                     /locus_tag="VF240_17910"
     CDS             2892..>4352
                     /locus_tag="VF240_17910"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEX7177139.1"
                     /translation="MRFPGTRPRRGEALAVLLALLVALAPVAAQTQDERDCDVRVTLL
                     QVNDVYQFLPVERGREGGLARVSTLRKEIARQSPHTMLLLSGDTISPSVESITYRGRQ
                     MIDAWNQVGLDYSVFGNHEFDFGPEELLARVGESKFKWLGANVIDKRTGRTFAETPPY
                     VVREFDGVKVGIFGITLPETKTTSSPGPNVEFRAACETANEIIPKLRDAGARAVVALT
                     HLSLAEDKELARCAEGIDVIIGGHEHTLLQSAAGGVPIFKMTADAREMGRIDLNIDPK
                     TGRVRSIDWEIIKVGADVKEDPEFAAVTRKYAAKIRELAGRVGRSSVALDARSAENRT
                     RETNVGNLVADSFRRATGADIALLNGGSVRADTVIAPGLLTMRDVLSILPFNNELEVI
                     EVTGALLLEALENGVGRTAPGAEPGRFPQVSGMKYSFDASLPAGQRIKSVTVGTRPLD
                     PARSYTLATTRYIAVGGDGYDMLQAAARVPRGKLIDS"
BASE COUNT          673 a         1442 c         1554 g          683 t
ORIGIN      
        1 ggttgcgaca acatcgcaac cggccccggc cttctgtttt aactgacctc taacctctaa
       61 cctctgacct cttcttcaga gcttcgggtg ttgttcgacg aagcggacga gcgcggcggc
      121 gtaagtggta aacgaggggc agcggacgcc gtgcggttcg aggagttcgg cggcgcgcgc
      181 ggtgtcgtag gtctgtttga tgaagaagta ggggacgccg gagtgtggga gtttggtgag
      241 ggtgtccgag agggggagcg tgagcgaggc gcgcacgagc ggggccggca cgatgaggcg
      301 cgagccgtgg ccggtcagca cgccggcgat gatgtcgaac aactcgcggg tggtgagcgg
      361 cgcggggtcg gcgagctgca cggtggcgcc ggccgcgcgc tcttcgcggg cgagcgcggc
      421 cattgcctcg acgacgaaat cgacgggcac gatgttgaga cgcacctcgt cgttgccgat
      481 gttggcgagc gcgagcaccc gcggcctgat ccgcagatac tggatgaggt agtagacgcc
      541 gtcgtacttc gccgtctcgc ccgtccggga gtcgccgcag acgaccgccg ggcggtggac
      601 ggtgacgggc agctcgcgtt taagcgcctc gacctcgacc tcggccaggt acttcgtctc
      661 ctcgtaataa tttcggaagc ccgcctcgtg ccgcagctcg tcctcccgga tcagccccgt
      721 gcgcttgccg gccacatagc acgtcgagac gtagtgatag cggcgcagat ggggaagcgt
      781 gcgcgcgaac tcgttgacgt ttcgcgtgcc ctcaaggttg acgagcacgg ccgcctcgcg
      841 cgcgacggcc agatcgtaca cggcggcgag gtgaaagacg gccgtcgtct cggagcgcgc
      901 gcgttcaagg tcggagggag agaggccgag ctggggccgc gtgatgtcgc cttctaaaat
      961 atgaaagttg cgggcgggcg tgcccgtctc ttcgacgatg ccggcgactt cggcgcgtgc
     1021 gcgctccgca agcgccggct ggacgagcaa caggaagcgc gcgccgtctt ccgcgagacg
     1081 acgcacgagc ctcccggcga tgaagccggg gaagccggtg aggaaaactg tcccgtcaaa
     1141 cttcacgttc aagcctcacg gccgcagacc agctcgacga gcgtgcggac gcccgcgccg
     1201 ctcgcgccct ggggggcgta ggggcgcgcc tcgtcgtacc aggcggtgcc ggcgatgtcg
     1261 aggtgtaccc agggggtgtc gccggcgaac ttgctgagca gcagggcggc gcccgtggcg
     1321 tcggggcggc cgccgtagtt tttgaagtcg gcgatgtcgc tctcgatgcg cttcttgtag
     1381 tcgtcggaga cgggcatccg ccacacgggt tcgccggcgc gctcggaggc cgcgcggagt
     1441 tgtccggccc acgcgtcgtc gttgctgaaa aggccggtgc ggtggtcgcc gagggcgacg
     1501 atgatcgagc cggtgagggt ggcgagatcg acgatgcgcg tcgcgccgag ttgtttcgcg
     1561 taggcgatgc cgtcaactaa gacgaggcgg ccctcggcgt ccgtgtccac gacctcgatg
     1621 gtgtggccgg cgagcgtgcg gacgatgtcg ccgggcttga tggccgtgcc cgagggcatg
     1681 ttctcggcgg cggggactat gcccacgacg cgccggcgtg gcttgagctg ggcgagggcg
     1741 cgcagggcgc cgagcgcggc ggcgccgccg gccatgtccg acttcatctc ctccatgccg
     1801 gggccacgct tcaaggagat gccgcccgtg tcgaaggtga tgcccttgcc gacgaaggcc
     1861 cacagctcgt cgtcggcgcg cgcaccttca ggctcgtagc gcaggacgat gagcgccggc
     1921 tcctcttccg acccctgcgc cacgccgagc agcgcgccca tcccgcgcgc gcgtatctct
     1981 tccgcaccca gcgtctcgat tcggaggccc acctccgcgg ccatcacggc ggcgcggcgg
     2041 gcgaactcgc gcggcggcaa actgaggccc ggctcgtcgg cgagcgtgcg cgcccagttg
     2101 accgactcgg cgacgatgcg cgcccgttcg acctcggcgc tcagttcaac acccccgcca
     2161 tgcggggcga cgatgacgag tcgttcgagc gcgggggccg gctcgtcccc attttgctgt
     2221 tgatagaagt cgttatcgta gaggccgagg agcgcgccct cgacgacggc gcgcgccgac
     2281 gcacgcgcgg ccgtcgcctc cacgccgggc gggaggacga agtgcaaatc tttgacgcgc
     2341 gcggcgcggg cctcgcgggc ggcgacgccc gccgcgcggc gcagcaccgc ctcgtcgaag
     2401 tgcgcgcgcg cgccgaggcc gacgagcagg acgcggcgca cgccgtcgct gccgcccgcg
     2461 ccccgcacgc gcagcatgag cgcggtctcc gcctcgccat cgaactcgcc gtcgcggacg
     2521 gcctgttggc agacgcgccc gagtacttcg tcgagacgcg cggcgtcgcc cggcgcgcgc
     2581 ggctcgtcgg cgaagacgca gacggccgcg gcctgtccgg gcgggttctc aggagggatt
     2641 tccgtggcga tacggatgga taagctgttc ggcttgttca tgcgctttcg acgccgggcg
     2701 cgggggcggc gggcgcggat tgaattaatt ctttcatgcg ggcgagtata attcagccct
     2761 tcggttcggc ctaacacgcc cgcggttaga gtgtgaactg acctgtgcta tcgttgaaaa
     2821 aacgtcgggc gcgcgcgcga aactaaggcg cgctcctttc ggtcgctact cattcaagcg
     2881 gaggacattc gatgaggttt cccggcacgc ggccccgccg cggcgaggcg ttggcggttc
     2941 tgctcgcgct gctcgtggcg ctcgcgcccg tcgcggcgca aactcaggac gagcgcgact
     3001 gcgacgttcg cgtgaccctc cttcaggtga acgacgtgta ccagttcctc cccgtcgagc
     3061 gcgggcgcga gggcggtctg gcgcgcgtct cgacgctgcg taaggaaatc gcccggcagt
     3121 cgccccacac catgctgctg ctctcgggcg acaccatctc gccctcggtc gagtcgatca
     3181 cctatcgcgg ccgacagatg atcgacgcct ggaatcaggt gggcctcgac tactcggtct
     3241 tcggcaacca tgagttcgac ttcggccccg aagagctgct cgcgcgcgtc ggcgagtcga
     3301 agttcaagtg gcttggcgca aacgtcatcg acaagcgcac gggtcggacg ttcgccgaga
     3361 cgccgcccta cgtcgtgcgc gagttcgacg gcgtcaaagt cggcatcttc ggcatcacgc
     3421 tgccggagac gaagaccacg tcgagtcccg gccccaacgt cgagtttcgc gccgcgtgcg
     3481 agacggctaa tgagattatc ccgaagctgc gcgacgcggg cgcgcgagcg gtcgtcgcgc
     3541 tcacacacct gtcgctcgcc gaggacaagg aactcgcccg ctgcgccgaa ggcatcgacg
     3601 tgatcatcgg cggccacgag cacacgcttt tgcagtcggc cgcgggcgga gtacccatct
     3661 tcaagatgac ggccgacgcg cgcgagatgg gccgcatcga cttgaacatc gatccgaaga
     3721 cggggcgcgt ccgctccatc gactgggaga tcatcaaggt gggcgcggat gtcaaagaag
     3781 accccgagtt cgcggccgtc acgcggaagt acgcggcgaa gatcagggag ctggccggac
     3841 gcgtcggacg ctcttcggtt gcgctcgacg cgcgcagcgc cgagaatcgc acgcgcgaga
     3901 cgaacgtcgg caacctcgtc gccgactcct tccgccgcgc caccggcgcc gacatcgcgc
     3961 tcctcaacgg cggctccgtg cgcgccgaca ccgtgattgc gcccggcttg ctgacgatgc
     4021 gcgacgtgct ctccatcctc cccttcaaca acgagcttga ggtgatcgag gtcacgggcg
     4081 cgcttcttct ggaggcgctg gagaacggcg tggggcgcac cgcgccgggc gccgagccgg
     4141 ggcgcttccc gcaggtgtcg ggcatgaagt acagcttcga cgcgagcctg cccgccggcc
     4201 agcggataaa gagtgtgacc gtgggcacac ggccactcga ccccgcacgg agttacacgc
     4261 tggcgaccac gcgctacatc gccgtcggcg gcgacggcta cgacatgtta caggccgccg
     4321 cgcgcgtgcc ccgcggcaag ctcatcgact cg
//
LOCUS       DASVES010000437        23188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_283780, whole genome shotgun sequence.
ACCESSION   DASVES010000437 DASVES010000000
VERSION     DASVES010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283780"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(403..1290)
                     /gene="argB"
                     /locus_tag="VF240_17915"
     CDS             complement(403..1290)
                     /gene="argB"
                     /locus_tag="VF240_17915"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7177140.1"
                     /translation="MSQQRLDLLREALPYIQRFKGKTFVVKLSGKVTEGRENLASLVE
                     ELALIHQVGIRVCVVHGGGKQLSALAAALGVEQTIIEGRRVTDDATLDMAKMIFAGKI
                     NIDILAALRRRGIDAVGLSGVDADIIHAVRRPPRDVLNRETGESEQVDFGHVGDVVET
                     NVRLLGILLDSGYLPVISSLGADPEGRVFNINADTVAAEIAAGLQAEKLILLSDVDGI
                     YLRPGDPASKLSSATAAELLALVRDRVATGGMIPKLQSIAALLGRGVRSTHVISGSAR
                     NALLAEVFTDEGTGTMIVA"
     gene            complement(1361..2407)
                     /locus_tag="VF240_17920"
     CDS             complement(1361..2407)
                     /locus_tag="VF240_17920"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HEX7177141.1"
                     /translation="MSINGQGQALPLQTMKNFLSTGDLTRDELEALLDGALRFKRGED
                     TSQPLAGRSVALVFFNPSLRTRASMQVGAYELGGHAVVLEPGGTAWTLEHREGAVMDG
                     DKTEHVAEFVRVLGRYVSMIGVRTFAAMKDWAEERRDPVLAAFAKYSEVPVVNLESAM
                     HHPCQSLADMLTVREKLGEGRKRVLLTWAWHPKPLPMAVPNSFALAAAQMGHDLTIAH
                     PPGYELDEDLIREINGRAFDAGARVRVTDDVEEAFDGAQVVYAKSWGSRQFYGEPQDD
                     IEERAKYRGEWIVDEAKMERTDSAVFMHCLPVRRNCIVTDAVIDSPASVVIDEAENRL
                     HVQKAIMKAVISDG"
     gene            complement(2459..3655)
                     /locus_tag="VF240_17925"
     CDS             complement(2459..3655)
                     /locus_tag="VF240_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7177142.1"
                     /translation="MMTQTISIFDDIAAREEEFQLATYKKFPFAVERGRGSWVETSDG
                     VRYLDLYGGHAVAGVGHCHPRVVAAIKEQAEALLFYSNVVHSGVRAAAAQKLAACAPA
                     ALGKVFFCNSGTEANENAMRMARMKTGREGVVTFTGGFHGRTADSISATFLGKYRELG
                     RPVVPGHVCAEFGEIEAVAAVADATVAAVMLEPIQSMAGVRTAAPEFFRALRRLCDER
                     GIVLIFDEVQTGVGRTGEWFFAGSEAAGGVVPDVLTLAKALGSGVPVGACVVTDEIAA
                     GIKENDLGTTFGGGMLAMAAVAATLEAIEADGMLENVKRVERHLRERLAGVAQVSGLR
                     GRGLLLGVEFREPSAAQVQKALLERRIITGTSSDPRVLRLLPPLCLTVEEADLFVDAL
                     NEAINL"
     gene            complement(3761..4789)
                     /gene="argC"
                     /locus_tag="VF240_17930"
     CDS             complement(3761..4789)
                     /gene="argC"
                     /locus_tag="VF240_17930"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7177143.1"
                     /translation="MKIRVGIFGGSGYGGAELLRILLFHPHAEVAFVTANEHAGKSVA
                     EVHKNLYGLTDLRFERAAEDVEQLRDLDCVFLALPHGHALDLAPRLPADVRVIDLSGD
                     YRLSDAGEFASHYGREHTSMELQTEFVYGLTEVNREAIKTARLVSNPGCFATATLLGL
                     APLAAHNLATGRVVVDAKTGSSGSGAKPAANTHHPQRMNSFYAYKPFAHQHVPEIEQT
                     LRRVGDWTNEFIFMTHSLPVARGIFASIYVETKEELTADAARDLYADYYRGSFFVRLV
                     SGSPDINWVKTTNFCDIGFAARGRQLAVFSAIDNLVKGAAGQAIQNMNLMFGLDEKTG
                     LMFAGGNP"
     gene            4891..5034
                     /locus_tag="VF240_17935"
     CDS             4891..5034
                     /locus_tag="VF240_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177144.1"
                     /translation="MRRQVGFPFLALGIALVAIGISSNRTFMYAGIAFLIISIVTLVR
                     GRR"
     gene            complement(5042..6127)
                     /locus_tag="VF240_17940"
     CDS             complement(5042..6127)
                     /locus_tag="VF240_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177145.1"
                     /translation="MSNAFEETLGTKVTVVEADKMGSAVSNLGLGKTLAVIGERRAAR
                     VGDVVVVRALTDSATYNQLELPSGRLAKINPGDVLVGVLGSRRALKGFVGDVPETVGE
                     GDELHLLNMGGVVGRCTGHHSALSDAIRLSVIGLVYDDDSRVANIADAALPLRAHLGE
                     TAPLVVVAGACMNSGKTFAAAEIVKQATRAGMRVAAAKLSGVACLRDTLNMADHGAVA
                     TASFLDAGLPSTVGVGDLAPVAKAVIARLNESAPDMIVVELGDGILGGYSVDSIFDDA
                     ELREATAALVFCASDYVGAWGGVELLRRRGVRVDLIAGSVTDSQMGEDYIEREFGVPA
                     ANAKRNGGRLFELIRQKVEAAPARVYA"
     gene            complement(6223..7434)
                     /locus_tag="VF240_17945"
     CDS             complement(6223..7434)
                     /locus_tag="VF240_17945"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX7177146.1"
                     /translation="MMTQKIKKVVLAYSGGLDTSAMLHWLRANYDCEVVCYCADVGQG
                     EELEGLAEKAERTGASKLYVEDLRQEFVEDYVWTAVKANAVYEGVYLLGTSLARPVIA
                     KRQIEIARLEGADAVAHGATGKGNDQVRFELTYYALEPGIKVIAPWREWEYKGRRDLI
                     DYCERHDIPVKATAEKPYSTDRNLMHVSYEGGILEDPWAEPPADIFQMTTAPEQARAE
                     AEYVEIGFERGAPVRLNGERMRAVALLERLNELGGAHGVGRVDLVENRFVGMKSRGVY
                     ETPGVTILHAAHRALESITLDREVLRLRDAMSLKFAESVYYGFWFAPETELMRKVIDE
                     TQVNVTGDVRMKLYRGNAIVAGRRSPHSLYSESLATFEAESVYNQRDAEGFIKLNALR
                     LRTQAQVSRKQ"
     gene            complement(7535..7954)
                     /locus_tag="VF240_17950"
     CDS             complement(7535..7954)
                     /locus_tag="VF240_17950"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177147.1"
                     /translation="MMEKEKRQQKVLSLIQTQNVGTQQELAALLERAGVAATQSSISR
                     DLVELGIVKRNGHYAIPQAAGGSPLRGLLAVAAAGDNLLVAKCEPGLASAVAVEIDRA
                     HIGEITGTLAGEDTIFVAVPNAKARRAAAKKIWELFQ"
     gene            8264..9652
                     /gene="argH"
                     /locus_tag="VF240_17955"
     CDS             8264..9652
                     /gene="argH"
                     /locus_tag="VF240_17955"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7177148.1"
                     /translation="MSESKKLWGGRFTGEADAGFARFNRSFGFDRRLFAADVRGSRAH
                     CEGLRAAGVLSSEEAERIDRGLQSLLERAAAEPDFLEDESAEDVHSFIEARLIEHIGD
                     AGRKLHTGRSRNDQVATALRLWLRDEIDRAVESLRGAQAALLDLAEAHPEACLPGYTH
                     LQRAQPILFAHWCLAYFEMLARDHERFVEVRRRTNVLPLGSAALAGTSHAIDRAGLAR
                     ALGFDSVSRNSLDAVSDRDFCVEFASAAALSMAHLSRLAEDLILYATTEFGFIELGDA
                     VSTGSSLMPQKKNPDALELVRGKSARVFGHLTALLTLIKGLPLTYNKDLQEDKEAVFD
                     TADTLRDALDVTAVVLRNIRLREGRAREAAAAGHMNATELADYLARRGVPFREAHEVV
                     GRAVLYAVGRGMELQELDLEELRRFSPHIDEDVYAALSLEETLATKRQAGGTAPERVS
                     EALAAARESLGH"
     gene            9870..10628
                     /locus_tag="VF240_17960"
     CDS             9870..10628
                     /locus_tag="VF240_17960"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX7177149.1"
                     /translation="MPRKNFALAFAFLLLSAVAVLAITYGQPDGNRHPNVGALVGTFS
                     GQTYPYCSGTLISPTVFLTAAHCYIGTTTVYVTFDTEYSSRSKLYTGTFYYDPLYNQS
                     QSDPHDIAVVVFDKPIRNIEPAQLPTLGQFDKLAHNQQFTAVGYGGEEPVITPGSGPV
                     IGYLDIRQYSTSTLNAVNPAWIRNSQNPATGDSGACYGDSGGPNFLGAGTTETNIIAG
                     TTITGDAICRATNVIYRLDTTSARNFLSAFVALP"
     gene            complement(10749..11951)
                     /locus_tag="VF240_17965"
     CDS             complement(10749..11951)
                     /locus_tag="VF240_17965"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7177150.1"
                     /translation="MRSFLSKTLVCVFAVLLAAVASGCRPDWFKGTRAEDKVRVGVFV
                     SLTGGTAGYGLSSMNGFRMAAEEINSSGGVGGRQVELLVEDTRSDALETARVVRRLVG
                     ERKVHALLGEVVSSRSLIAAPIAQAAGVAMLTPSSTNLEVTERGDYIFRSCYTDPFQA
                     NALARFAADGLRAKRAALLIDRSQSYSVELAELFREAFTRRGGQVVSEQPYSEGEEDF
                     TQQLMQAGSASPDVIFVPGYYREAGLIARQAKELGIDAPLVGGDGWDSPGLYHIGGEY
                     LRGSFFSNHFSVDDKDPAVQSFVEDYLGLYGYPPDAFAATAYDATRILLHAVRDAGSL
                     DRRSIRDALASTAGFKGVTGTITFGPDRNAIKPIVIIRIETGGLYAVQERLMPQPSAN
                     LLGSSSQK"
     gene            complement(12173..12877)
                     /locus_tag="VF240_17970"
     CDS             complement(12173..12877)
                     /locus_tag="VF240_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177151.1"
                     /translation="MLTLENVSVSYGAIKALKGVSMRVERGEVVTLIGANGAGKTTTL
                     RTITGLLSPTEGRVVFEGDDVSGTPTHKLVARGISMSPEGRGVFANLTVRENLQMGAY
                     LKKNKKEIAEDMERAFRMFPRLKERESQKAGTLSGGEQQMLAMGRALMSRPRLLLLDE
                     PSLGLAPLVVHTIFEAIEEIRGEGTTILLVEQNAHAALGHSDRAYVLETGNIVMEGPS
                     KDLAADPRIKEAYLGE"
     gene            complement(12971..13807)
                     /locus_tag="VF240_17975"
     CDS             complement(12971..13807)
                     /locus_tag="VF240_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177152.1"
                     /translation="MTPNAGTTHAGGATDAAAAMVDRGFGAPILKTTQCTIRFGGLVA
                     VNALDMAVRPGEIYGLIGPNGAGKTTIFNLLTGVYRPTSGTIEFEDKRVDGLRPYEIA
                     ALGVSRTFQNIRLFPSMSVLENVMTACHIHTQQTLADAVLRTPRFARDEREHEQFCLE
                     LLEIFDLARFRDEGGTSLPYGNQRRLEIVRALATRPRLLLLDEPAAGMNPSEQDDLLK
                     LIRQIRDRFQLTILLVEHNMKVVMGVCERIQVVDYGKSIALGLPEEIRNDPKVIEAYL
                     GD"
     gene            complement(13804..14910)
                     /locus_tag="VF240_17980"
     CDS             complement(13804..14910)
                     /locus_tag="VF240_17980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7177153.1"
                     /translation="MPRYARNLVVAFLLLAVLYALDYFVFDPGRRMISPYVARIIMLA
                     GINITLAVSLNLINGFTGQFSIGHAGFMAVGAYSSAYFSVSYGAAMARSLGDGLLGWG
                     AALLVATLLGALVAGAAGFLVGVPSLRLKGDYLAIVTLGFGQIIVVFLNNIEAIGGAR
                     GYSGIPIVKSFFWIALVAVLTIVVVHNLVHSAIGRALVSIREDELASEAMGVNTTRYK
                     VMAFVISSALAGAGGVLLAHFDGYLNPKSFEFIKSFEILIMIILGGLGSIVGSVLGAI
                     VLTVLPEALREFAQYRMVIYSILLIVLMITRPNGLLGGLDPFKGWRARNRAKTRTEAE
                     EITAEDTRDQTDVDKATLGERARDTSLGEGDKRP"
     gene            complement(15049..16017)
                     /locus_tag="VF240_17985"
     CDS             complement(15049..16017)
                     /locus_tag="VF240_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003835684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7177154.1"
                     /translation="MSTFIQQLINGISLGAIYSLIALGYTMVYGVLKLINFAHGDVYM
                     VGAFMGYYLANGLGAQWAQKLGQDPNGVFARGLLGSGALEPSLITALVVMLLAMAICA
                     ALGVIIERLAYRPVRKYSRLTALITAIGVSLLLENGGQVVFGADPKFFPELFTKQNID
                     LGGGVSVNSSDIVVLVIAVGLMVVLQYIVYYTKTGKAMRAVSFNITSAKLMGINTDYI
                     IAFTFALGSALAAAAGVLVAIRIPRIDPLMGILVGLKAFVAAVLGGIGNIPGAMLGGL
                     LIGITETLVVGYLSPTYKDAVAFAILILVLLFRPSGLLGSVAQEKV"
     gene            complement(16216..17547)
                     /locus_tag="VF240_17990"
     CDS             complement(16216..17547)
                     /locus_tag="VF240_17990"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7177155.1"
                     /translation="MKRTLIALGLTASMLAATACQTGGPGGGTAGGDKVKIGVFMSLT
                     GSTAQFGISSTNGIKMAADEVNAAGGINGKQIELLVQDDRSDAQEAATIVTKFVTQDG
                     VHAVLGEVASSRSIAAAPIAQNAKIPMLTPSSTNPDVTKAGDYIFRSCFIDPVQGAAM
                     SQFAGKSLGKRRAALMVDRKNDYSTGLEKVINEVFPRFGGEVVITQSFQEGDTDFNGQ
                     LTSIKGANPDVIFIPGYYGDVALIAKQARDKGLNVPLVGGDGWDSAQLYTIGGAAVVG
                     SYFTNHYSPYDTDAKVQKFVQGYQQRYGGIPDALAATAYDAALIMFDAIKRAGSLDGK
                     AIRDALAATKDFPGVTGTVNFNANRDAVKPIMMIRVDEGGKYVIQERVSVEGASMPTE
                     AGAANTNTNAAPASNANMATGNANTGANINININTNAGATNTNARPANVTP"
     gene            18057..19565
                     /locus_tag="VF240_17995"
     CDS             18057..19565
                     /locus_tag="VF240_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX7177156.1"
                     /translation="MKQTESETGGLAPPLGDMSAEEFRRHGHELVEWIAGFLERLEDF
                     PVLAQVRPGDLKSQLPQSPPAEGEPMPEILADVERLVLPALTHWNHPSFFAYFATSSS
                     APGIFGELLSAVFDVKSMLWRTSPASTELEEVTLSWLRQMMGLPAEFDGLIYDTASVA
                     TMHAVAAAREALDMRVREDGMSGRADLPLLRLYSSEQAHSSVEKALLALGLGQRSLCK
                     VAADSEFRMKPDALAEAIDEDRRAGMLPFCVVATVGTTSTSSIDPVAEIADVCERERL
                     WLHVDAAYAGSAAVVPELRPLFAGCERADSFVVNPHKWLFTPFDLSALYTRRLDMLRR
                     AFTLTPEYLRTPEAETVRHGSDYGVQLGRRFRALKLWMILRYFGHDGLAARIREHCRL
                     ARLFASWVAETPGWELLAPVPFGLVCFRACPVADDETEAARGARLDALNESLMNSVNA
                     TGQMFLSHTKLNDVFTLRLSVGNIRTTEAHVRRAWELLNEHAARLADSPSSV"
     gene            19621..21783
                     /locus_tag="VF240_18000"
     CDS             19621..21783
                     /locus_tag="VF240_18000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7177157.1"
                     /translation="MRSTLAILALLASLTVAVSLKAQTRRGMTPEDTLRFAEVGDAQI
                     SPSGEWVVYVVATTEGEERRSALWLVRAGPGAVTSVPGFVQESPDASRSVIAPTRINT
                     AGLNASRPRWSPDGHRLAFVGSGGGRPSGVWVVSSFERESQPRLVAVVRDSNFHVPYA
                     GESFAWSPDSRRIAYITATEEADDGERRPTVAGARNDDPRVLTRVRYKSRTSFSDTLR
                     THVWLAEVDDPAAQPRQLTSGPFHDHAVSFNPRADEIAFLSNREPDPDAVNNSDIYAV
                     STDGRTRRVTETRGCEYDPQWSPDGRLIAHTATTRDVTTIDSVSEDAHVWVVESGGGG
                     RREISAALDRRARSPRWSADGRSVLFLAGDAGRTLLFRAPISGGPVARIHFDRADDSP
                     LRNLFASPGAPQPQPFQLGNFSVSARRPSLFAFTLSTPLHPSEVWAGESTEGDARRLS
                     AHNDVLLRTLSLSAPEVFSVRSFDGAQVQAWLMRPAAFRPSSGQRHPVVLSVHGGPHG
                     AYGYGFNAEFQTYAARGYAVLYMNPRGSTGYGQKFSDGTFREWGGGDYRDLLAGVDEA
                     LSKYSWLDPERMGVVGGSYGGFMVNWIITQTPRFRAAVARASVSNLVSFYGTSLYQDL
                     IQAEFGQMPWDDYDLLWRWSPLRYVRDARTPTLFIHGELDNDVHITQAEEMYTALRRR
                     GVEAALVRYPREGHGFREPRHRRDSIERALDWFDRFLK"
     gene            21896..22210
                     /locus_tag="VF240_18005"
     CDS             21896..22210
                     /locus_tag="VF240_18005"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX7177158.1"
                     /translation="MLTHIVIWKYREDVGEEERRLHVEMLRRLPVLIPEVLSFHVGFD
                     VLRLPRSYHTGLASTFRDRRGLEAYTVHPEHVAVADFGRRISEHVASVDFEEDKDVNR
                     KS"
     gene            22318..>23188
                     /locus_tag="VF240_18010"
     CDS             22318..>23188
                     /locus_tag="VF240_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177159.1"
                     /translation="MKRWKKIALVVVGLLILSQAPFVYRRYRLGRLRAAIDSVNASRA
                     AAVADDPFVDYKGVLHVHSSLGGHSTGTLDEIVAAARANELAFVVMTEHPSKYVDTVA
                     ATLKGTHEGVLFINGSELSAANDERLFVVPGPSVPAAGPAAPNAQALVERARAEGRLA
                     LVAYPERIRDWGLLQGYDGIEVYNLYTNSKRIRYGLLFFDGLWSYRSHADLLFSTFYE
                     RPDENLRRWDELTAARGLRLSAVAGNDAHSNVGLRLGDRTGKEFLGVSLDPYERSFRV
                     VRNHVLLDRAQPLA"
BASE COUNT         3906 a         7889 c         7337 g         4056 t
ORIGIN      
        1 gaacctcgcg gtcgaagaac tggttcgcat gcgcgaccac ggcgtcacgg cctccttcgt
       61 ccgccgcgtc aaggacgagc gcggcgccgc cccggcggtc gatgagctga tccgcatcaa
      121 gaaccgcggc tcatactgag gattgttcgc caatgagcgt gaaacaattt cgcgcttcaa
      181 cagccccgtc aggggcggca gaatgtagcc cacggcgtga gccgtgggcg atggcaataa
      241 aaacaacgga agccccggag gggcggaaga atctcaacga gcaacatgct cgggcttgag
      301 attcttccgc ccctccgggg ctttatcgat tttgttccgc gttcaccccg gtcttgcgac
      361 cggggctaca atctttcacc cctgacgggg cttgtctctt cattacgcca cgatcatcgt
      421 tcccgtgccc tcgtcggtga agacttcggc gaggagggcg ttgcgggcgg agccgctgat
      481 gacgtgtgtg ctgcggacgc cgcggccgag gagggcggcg atggactgga gtttgggaat
      541 catgccgccg gtggcgacgc ggtcgcggac gagcgcgagc agttccgcgg ctgttgcgct
      601 agagagcttc gacgcggggt cgccggggcg caggtagatg ccgtccacgt cgctcaggag
      661 gatgagcttt tcggcctgga ggccggcggc gatctcggcg gccacggtgt cggcgttgat
      721 gttgaagacg cgcccctcgg ggtcggcgcc gagcgaagag atgacgggca ggtagccgct
      781 gtcgaggagg atgccgagca agcggacgtt cgtctcgacc acgtcgccga cgtgcccgaa
      841 gtccacttgc tcgctctcgc ctgtctcccg gttgagcaca tcgcgcgggg gccgccgcac
      901 ggcgtggatg atgtcggcgt ccacgcctga gaggccgacg gcgtcgatgc cgcgccggcg
      961 cagcgccgcg aggatgtcga tgttaatctt gccggcgaaa atcatcttcg ccatgtcgag
     1021 cgtggcgtcg tccgtcacgc gccggccttc gatgatcgtc tgctcgacgc ccagcgccgc
     1081 ggccagcgcc gacaactgtt tgccgccgcc gtgcaccacg cacacgcgga tgcccacctg
     1141 atggatcagc gccagctcct cgaccagact ggcgaggttc tcgcgaccct ccgtcacctt
     1201 ccccgacaac ttcacgacga aggtcttgcc cttgaaccgc tggatgtacg gcagggcctc
     1261 gcgcaacaga tcgagtcgct gctggctcat ttcttctcca ggtgataagt gatgagtgat
     1321 aagtgataag aaagagaaag caggtttttc ttatcactca tcacccatca cttatcactg
     1381 ctttcatgat tgccttctgg acgtgcaaac ggttctcggc ttcgtcgatg acgacggagg
     1441 cgggcgagtc gatgacggcg tcggtgacga tgcagttgcg tcggacgggg aggcagtgca
     1501 tgaagacggc gctgtcggtg cgttccatct tcgcctcgtc cacgatccac tcgccgcgat
     1561 atttggcgcg ctcctcgatg tcgtcctgcg gctcgccgta gaactgccgg ctgccccagc
     1621 tcttcgcgta gacgacctgc gcgccgtcga acgcctcctc cacgtcgtcc gtgacgcgga
     1681 cgcgcgcgcc cgcgtcgaag gcgcggccgt tgatctcgcg gattaaatct tcgtcgagtt
     1741 catagccggg cgggtgggcg atggtcaggt cgtggcccat ctgcgcggcc gcgagcgcga
     1801 agctgttggg gacggccatc ggcagcggct tcgggtgcca cgcccacgtc agaagcacgc
     1861 gcttgcgccc ctcgcccagt ttctcgcgca ccgtcagcat gtcggccaac gactgacacg
     1921 ggtgatgcat ggccgactct aaattcacga cgggcacttc cgaatatttc gcgaaggcgg
     1981 cgagcacggg gtcgcggcgc tcctcggccc agtccttcat cgccgcgaag gtgcggacgc
     2041 cgatcatcga gacgtagcgg ccgagcacgc ggacgaactc ggcgacgtgc tcggtcttgt
     2101 cgccgtccat cacggcgccc tcgcggtgct ccagcgtcca cgccgtcccg cccggctcca
     2161 acacgaccgc gtggcccccg agttcatagg ccccgacctg catcgaggcg cgcgtccgca
     2221 aagaagggtt gaagaagacg agcgcgaccg agcggcccgc gagcggctgc gacgtgtctt
     2281 cgccgcgctt gaagcggagc gcaccgtcaa gcagagcctc aagctcgtcg cgtgtcaggt
     2341 cgccggtaga tagaaaattc ttcattgttt gtaggggcag agcttgtccc tgcccgttga
     2401 tactcattga tattcaagct gcgcttccac ggacacgggc cgcggacaag ccctgcccct
     2461 acaggtttat cgcctcgttc aatgcatcga cgaataggtc ggcctcttca accgtgaggc
     2521 agagcggcgg caggagccgg agcacgcgcg ggtcgctcga cgtgccggtg atgatgcggc
     2581 gttcgagcag cgccttctgc acctgcgcgg ccgacggctc gcggaactcg acgccgagca
     2641 gaagcccgcg ccccctcaag ccagagacct gcgcgacgcc cgcgagacgc tcgcgcagat
     2701 gtcgctcgac gcgcttcacg ttttcgagca tcccgtcggc ctcaatcgct tcgagcgtcg
     2761 ccgcgacggc cgccatcgcc agcatccccc cgccgaacgt cgtgccgagg tcgttctcct
     2821 tgatgccagc ggcgatctca tcagtcacca cacaggcccc gacggggacg ccgctgccga
     2881 gcgccttcgc gagcgtcaac acatccggca caacgccgcc cgccgcctcg ctccccgcga
     2941 agaaccactc gcccgtgcgg cccacacccg tctgcacctc gtcgaagatg agcacgatgc
     3001 cgcgctcgtc gcacagccgc cggagcgcgc gaaaaaactc cggcgcggcc gtccgcaccc
     3061 cggccatcga ctgtatcggc tcaagcatca cggccgccac agtcgcatcc gccaccgccg
     3121 ccaccgcctc gatttcgccg aactccgcgc acacgtggcc gggcaccacg ggccgcccga
     3181 gttcgcgata cttacccaaa aacgtcgcgc tgatcgagtc ggccgtgcgc ccgtggaagc
     3241 cgcccgtgaa ggtgacgacg ccctcgcggc cggtcttcat gcgcgccatc cgcatcgcgt
     3301 tctcattggc ctccgtgccc gagttgcaga agaagacctt gccgagcgcc gccggcgcgc
     3361 acgcggcgag tttctgggcg gcggcggcgc gcacgcccga gtggacgacg ttcgagtaga
     3421 acagaagcgc ttccgcctgc tctttaatgg ccgcgacgac gcgcgggtga caatggccga
     3481 cgcccgcgac cgcgtgcccc ccgtagaggt cgagataacg cacgccgtcc gaagtctcca
     3541 cccacgaccc gcgcccgcgc tcgaccgcga agggaaactt cttgtacgtc gcgagctgaa
     3601 actcttcctc gcgcgccgcg atgtcgtcga atattgaaat cgtctgtgtc atcatctttt
     3661 tcgagtaagc agtgagcagt gagcagtgag cagtttaaag aaccgccagc ggcagaagtt
     3721 ttttaactgc tcactgctca ctgctcactg cttactgtct tcaaggattc ccgccggcga
     3781 acatcagccc cgttttctcg tcgaggccga acatcaggtt catgttctgg atggcctggc
     3841 cggcggcgcc tttgacgagg ttgtcgatgg cggagaagac ggcgagctgc cggccgcgtg
     3901 cggcaaagcc gatgtcgcag aagttcgtcg tcttcaccca gttgatgtcg ggcgagccgg
     3961 agacgaggcg gacgaagaag cttccgcgat agtagtccgc gtagaggtcg cgcgccgcgt
     4021 cggccgttag ctcctccttc gtctcgacgt agatcgaagc gaagatgccg cgcgcgaccg
     4081 gcagcgagtg ggtcataaag ataaactcgt tcgtccaatc gcccacccgc cgcaaagtct
     4141 gttcgatctc ggggacgtgc tggtgcgcga agggcttgta ggcgtagaag gagttcatgc
     4201 gctgcgggtg gtgcgtgttg gcggcgggct tcgcgcctga ccccgaagag ccggtcttgg
     4261 cgtccacgac gacgcgcccc gtggcgagat tatgcgcggc gagcggcgcg aggccgagga
     4321 gcgtggcggt ggcgaaacag ccggggttgc tgacgagccg cgctgttttg atggcctcac
     4381 ggttgacttc tgtcaggccg tagacgaact ccgtctgcaa ctccatcgac gtgtgctcgc
     4441 ggccgtagtg gctggcgaac tctccggcgt ccgaaagacg gtagtcgccg gagaggtcga
     4501 tgactcgcac gtccgccggg agacgcggcg caagatcgag cgcgtgaccg tgcggcagcg
     4561 cgaggaagac gcagtcgagg tcgcgaagtt gctccacgtc ttcggccgcg cgctcgaaac
     4621 gcagatcggt caggccgtaa agatttttat gaacctcggc gaccgatttg cccgcgtgct
     4681 cgttggccgt cacgaacgcg acttcggcgt gcgggtgaaa aagcaggatg cgcaggagtt
     4741 ccgccccgcc gtagcccgag ccgccgaaga tgcccactct gatcttcatt tccctcaccc
     4801 cgaacattta cccgcgctcg ggcgtaaaca atcttgactc aacacgccgc agcggtcggc
     4861 ccttttcaac caccgggaga ttacacggaa atgcgtagac aagtcggatt ccccttcctc
     4921 gccctcggca tcgccctcgt cgccatcggc atcagctcta acagaacttt catgtacgcc
     4981 ggcatcgcct tcctcatcat cagtatcgtc accctcgtcc gcggccggcg ttagacgccg
     5041 ctcacgcgta gacgcgcgcc ggcgccgcct cgaccttctg acggatcaat tcgaagagac
     5101 gaccaccgtt gcgtttggcg ttggccgcgg gcacgccgaa ctcgcgctcg atatagtcct
     5161 cacccatctg cgagtctgtc accgacccgg cgattaagtc cacgcgcacg ccgcgccggc
     5221 gcagaagctc gacgccgccc cacgcgccca cgtagtccga cgcgcagaag acgagcgcgg
     5281 ccgtcgcctc gcgcaactcc gcgtcgtcga agatggaatc gaccgagtaa ccgcccagaa
     5341 ttccgtcgcc caactcgacg acgatcatgt cgggcgccga ctcgttgagg cgcgcgatga
     5401 cggccttcgc cacgggcgcg aggtcgccga cgccgacggt cgaaggcagc cccgcgtcga
     5461 ggaagctggc ggtggcgacg gccccgtggt cggccatgtt gagcgtgtcg cgcagacagg
     5521 ccacacccga gagcttggcc gccgccacgc gcatccctgc gcgcgtcgct tgcttgacga
     5581 tctcggcggc cgcaaaagtc ttccccgagt tcatgcacgc gcccgccacg acgacaagcg
     5641 gcgccgtctc acccaaatgt gcgcgcaggg gcagcgccgc gtcggcgatg ttcgcgacgc
     5701 gcgagtcatc gtcgtagacc agtccgatga cgctcaagcg aatcgcgtcg ctcaaagcgg
     5761 aatggtgtcc cgtgcagcga ccgacgacgc cgcccatgtt caaaagatgc agctcgtcgc
     5821 cttcgcccac ggtctccggc acgtcgccca cgaagccctt gagcgcgcgg cggctgccga
     5881 gcacgccgac gagcacgtcg cccgggttga tcttcgccag ccggcccgac ggcaactcaa
     5941 gctgattgta ggtcgcgctg tccgtaagcg cgcgcaccac gaccacgtcg cccacacgcg
     6001 ccgcgcggcg ctcgccgatc acggccagcg tcttccccag cccgaggttc gacacggccg
     6061 accccatctt gtcggcctcg acgaccgtca ccttcgtgcc cagcgtctct tcaaatgcgt
     6121 tcgacatctt tacctccggt aagtgtgtag taagcagtaa gcagtgagca gtgagcagtt
     6181 aaaactttct gcccctcgcg gctctttttt accgctcact gcttactgct tacggctcac
     6241 ttgcgcttgc gtccgcaagc gcagcgcgtt gagcttgatg aatccctcgg cgtcgcgctg
     6301 gttgtagacc gactcggcct cgaacgtggc gaggctctcg ctgtagagcg agtggggcga
     6361 gcggcgtccc gcgacgatgg cgttgccgcg gtagagcttc attcgcacgt cgcccgtgac
     6421 gttcacctgc gtctcgtcaa tcacctttcg catcagctcc gtctcgggcg cgaaccagaa
     6481 gccgtagtag acgctctcgg cgaacttcag actcatcgcg tcgcgcagcc gcaacacctc
     6541 acggtcgagc gtgatcgatt cgagcgcgcg gtgcgccgcg tgcaggatcg tcacgcccgg
     6601 cgtctcgtaa accccgcgcg acttcatccc gacgaaacga ttctccacca gatcgacgcg
     6661 tcccacgccg tgcgccccgc ccaattcgtt gagcctttcg agaagcgcca ccgcccgcat
     6721 gcgctcgccg ttcagacgga cgggcgcacc ccgctcgaag ccgatctcaa cgtactcggc
     6781 ttccgcccgc gcctgctcgg gcgccgttgt catctggaag atgtcggcgg gcggctcggc
     6841 ccacgggtct tcgaggatgc cgccctcgta ggagacgtgc atcaggttgc ggtcggtcga
     6901 gtagggcttc tcggccgtag ccttcacggg gatgtcgtgc cgctcgcagt agtcgatcaa
     6961 atccctgcgg cccttgtact cccactcgcg ccacggggcg atgaccttga tgcccggctc
     7021 cagcgcgtag taggtcagct cgaagcgcac ctgatcgttg cccttgcccg tggccccgtg
     7081 cgcgacggcg tcggcccctt cgagccgggc gatctcgatc tggcgcttgg cgatgacggg
     7141 tcgcgcgagc gaggtgccga gcagatagac gccctcgtag acggcgttcg ccttgacggc
     7201 cgtccacacg tagtcctcga caaactcctg ccgcaggtct tcgacataga gcttcgacgc
     7261 tcccgtccgc tcggcctttt cggcgagacc ttccaactcc tcgccctgcc ccacgtcggc
     7321 gcaataacag acgacctcgc agtcgtaatt cgcgcgcagc cagtggagca tggccgaagt
     7381 gtccagcccc cccgaatacg ccagcaccac ctttttgatc ttttgtgtca tcattcttta
     7441 gtagggagtc agaagtcaga agtcaggagt cagaataaaa accgtccgtc cgctttcttc
     7501 attctgacta ctgacttcag actcccgact cctgttattg aaacaactcc caaatcttct
     7561 tcgccgccgc gcgccgcgcc tttgcgttcg gcacggcgac gaagatcgtg tcttcaccgg
     7621 cgagcgtccc tgtgatctcg ccgatgtgcg cgcggtcgat ctcgacggcg acggccgacg
     7681 ccagccccgg ctcgcacttc gccaccagca ggttgtcgcc cgcggccgcg accgcgagca
     7741 gccctcgcag cggcgatccc ccggccgcct gcgggatcgc gtaatggccg ttgcgcttga
     7801 cgatgcccag ttccacgagg tcgcgcgaga tgcttgactg cgtggcggcc acgccggcgc
     7861 gctccagcag cgcggccaac tcctgctgcg tgcccacgtt ctgagtctga atcaggctca
     7921 ggactttctg ctgtcgcttt tctttctcca tcatcctgtt atgcaaatat tgtgcatatt
     7981 taatgcatac ttgcataaac tagcatttta gatgcacaat cgcaaccggg gccgggattt
     8041 tttttagctg gggcgaaggc gggcgcgccc tgagcgcggg acagtaccgt gagcggtagc
     8101 gagcgggtct catgcacgcg aggcgagccg ctgacatagg cgcacccgct cgctaccgct
     8161 cgcggtactg tccgcactgc caattcttct ggcgcctttc gcttcgcgat agttgcgttt
     8221 atcttttcaa ccgtgcggga gaggacgttc aggaagcatt caaatgagcg aatcgaaaaa
     8281 actctggggc ggcaggttca cgggcgaagc ggacgccggg ttcgcgcgct ttaaccgctc
     8341 gttcggcttc gaccgcagac tcttcgcggc ggacgtgcgc ggcagccgcg ctcactgcga
     8401 ggggttgcgc gcggccggcg tgctcagcag cgaggaagcc gagcgcatcg accgcggtct
     8461 tcagtcgctt ctcgaacgcg ccgccgccga gccggacttt ctcgaagacg agtcggccga
     8521 ggacgtacac tcgttcatcg aggcacggct catcgaacat attggcgacg cgggccgcaa
     8581 actccacacg ggccgcagcc gcaacgatca ggtagccacg gccctgcgcc tctggctgcg
     8641 cgacgagatc gaccgcgccg tcgagagcct gcgcggcgca caggccgcgc ttctcgattt
     8701 ggccgaggcc caccccgaag catgtctccc cggctacacg cacctccagc gcgcgcagcc
     8761 catcctgttc gcccactggt gtctcgccta tttcgagatg ctcgcgcgcg accacgagcg
     8821 cttcgtggaa gtgcgtcgcc gcacgaacgt cctgccgctc ggctcggcgg cgctcgcggg
     8881 gacgagccac gccattgacc gcgcggggct cgcgcgcgcg ctcgggttcg actccgtctc
     8941 gcgcaacagc ctcgacgccg tcagcgaccg cgacttctgc gtcgagttcg cgtccgccgc
     9001 cgcgctctcg atggcgcatc tctcgcgcct cgccgaagac ctcatcctct acgcgacgac
     9061 cgagttcggc ttcatcgaac tcggcgacgc cgtctccacg ggttcgagcc tgatgccgca
     9121 gaagaagaac ccggacgcgt tggagcttgt gcgcgggaag tcggcgcgcg tcttcggcca
     9181 cctgacggcg ctgctcacac tcatcaaggg gctgccgctc acttacaaca aagacttgca
     9241 ggaggataaa gaggccgtct tcgacacggc cgacacgctg cgcgatgcgc tcgacgtgac
     9301 ggcggtcgtg ctccgcaaca tccgcttgcg cgaggggcgc gcccgcgagg ccgccgccgc
     9361 cggacacatg aacgcgaccg agttggccga ctacctcgcg cgtcgcggcg tgcccttccg
     9421 cgaggcgcac gaagtagtgg ggcgcgccgt gctctacgcc gtcgggcgcg gcatggagct
     9481 acaggagtta gacctcgaag agctgcgccg gttctctccg cacatcgacg aggacgtgta
     9541 cgccgcgctc tcgctcgaag agaccctcgc gaccaagcgg caggcgggcg ggacggctcc
     9601 cgagcgcgtc tcggaggcgc tggcggcggc gcgggaatca ctaggccact agagtgatgt
     9661 ttacttgagt gaatgccgaa ttgattcacc gcggaggcgc cgcagggcgc agaggggttg
     9721 gaagctgaac gggattcgct gtttctaaag tcgcatacga cttgacccgc cgcctcgcgg
     9781 gggttaactt tgcctcccac ttcagagtac gcccccggcg gcggggactg ctaaaccaca
     9841 tttccgtcaa aagaattaag gagcctccta tgcctcgtaa aaactttgcg ctcgcgttcg
     9901 cgttcctcct cctctcggct gtcgccgtgc tggcgatcac ctacgggcag cccgatggca
     9961 accgccaccc gaacgtcggc gcactcgtcg gcaccttcag cgggcagacc tacccctatt
    10021 gctccggcac gctcatctcc ccgaccgtct tcttgacggc cgcgcattgc tacatcggca
    10081 cgacgaccgt ctacgtcacc ttcgacaccg agtactcgtc gcgctcgaag ctctataccg
    10141 gcaccttcta ctacgacccg ctctacaacc agtcgcaatc ggacccgcac gacatcgccg
    10201 tcgtcgtctt cgacaagccg atccgtaaca tcgagccggc gcagttgcca acgctcgggc
    10261 agtttgacaa actggcgcac aaccagcagt tcacggccgt cggctacggc ggagaggagc
    10321 cggtcatcac gcccggcagc ggccccgtca tcggctacct cgacatacgg cagtactcca
    10381 cctccacttt gaacgcggtc aacccggcgt ggattcgcaa ctcgcagaac ccggcgaccg
    10441 gcgacagcgg cgcctgttac ggcgactcgg gcggcccgaa cttcctcggc gccggcacga
    10501 ccgagaccaa catcatcgcc ggcacgacca tcaccggcga cgccatctgc cgcgccacca
    10561 acgtcatcta tcgcctcgac accacatcgg cgcgcaactt cctgagcgcc ttcgtcgcgc
    10621 tgccgtagct gcggataaga agacgcacgc ttgaccgtcg cggcgcggcg gggaacacgg
    10681 ataagacgga tgggacggat cgacacggag caaccgtgaa catccgtccc atccgtctta
    10741 tccgtgttct acttctgact cgatgatccc aacaggttcg ccgacggctg cggcatcagg
    10801 cgctcctgca cggcgtacag tcccccggtc tcgatgcgga tgatgacgat gggcttgatg
    10861 gcgttgcggt cggggccgaa ggtgatggtg ccggtcacgc ccttgaagcc ggccgttgag
    10921 gcgagcgcgt cgcgtatcga acggcggtcg agcgagccgg cgtcgcgcac ggcgtggagg
    10981 aggatgcgcg tcgcatcata ggcggtggcg gcgaaagcgt cgggcggata gccgtagagg
    11041 ccgaggtagt cttcgacgaa gctctgcacg gccgggtctt tgtcatccac ggagaagtgg
    11101 ttcgagaaga aggagccgcg caggtactcg ccgccgatgt ggtagaggcc gggcgagtcc
    11161 cagccgtcgc cgccgacgag cggcgcgtcg atgcccagct ctttcgcctg ccgggcgatg
    11221 agccccgcct cgcggtagta gccggggacg aagatcacgt cgggcgaggc cgagccggcc
    11281 tgcatgagct gctgcgtgaa atcctcctcg ccctcactgt aaggctgctc ggagaccacc
    11341 tgcccgcccc ggcgcgtgaa cgcctcgcgg aacagttcgg ccagctcgac cgagtacgac
    11401 tggctgcggt cgatgaggag agcggcgcgt ttggcgcgca ggccgtcggc cgcaaaacgc
    11461 gccagtgcgt ttgcctgaaa ggggtcggtg tagcagcttc ggaagatgta gtcgccgcgc
    11521 tcggtgactt cgaggttggt tgaggagggc gtgagcatgg cgacgccggc cgcctgcgcg
    11581 atgggcgcgg cgatgagcga gcgcgagctg acgacctcgc cgaggagcgc gtgaactttg
    11641 cgctcgccca ccaagcggcg gacgacccgc gcggtctcca aagcgtcgga gcgcgtgtcc
    11701 tccaccaaca gctcgacctg ccggccgccg accccgcccg atgaattgat ctcctcggcg
    11761 gccatgcgga agccgttcat cgacgacaga ccgtaaccgg ccgtgccccc cgtcagcgag
    11821 acgaagacgc cgacgcgtac cttgtcttcg gcgcgcgtcc ctttgaacca atccggccga
    11881 caacccgaag ccacggccgc gagcaggacg gcgaacacac agaccagcgt cttcgacagg
    11941 aagcttctca tcactttctt tacaactctc ggtcgagtga atggttcgac cctccgcgca
    12001 acctccgcgc cctccgcgcc tccgcggtga gtcgaactcc ccatccactc aatcaagtaa
    12061 ttcatctgcg cgcccacggc gacgctcacg cgggcattct atcacccgaa aactaaagag
    12121 gccccgcacg tgcggagcct cgcgtagatg ccagagaagc gagcgcgcgc gtctattcgc
    12181 cgaggtaagc ctccttgatg cgcgggtcgg cggccaaatc cttcgacggc ccctccatga
    12241 cgatgttgcc ggtttcgagg acgtaggcgc gatccgagtg gccgagcgcg gcgtgggcgt
    12301 tctgctcgac gaggaggatg gtcgtcccct cgccgcgaat ctcctcgatg gcctcgaaga
    12361 tggtgtggac gacgagcggg gccaggccca gagaaggctc gtcgagcagg agcaggcggg
    12421 gccgggacat cagcgcgcgc cccatcgcga gcatctgctg ctcgccgccc gagagcgtgc
    12481 cggccttctg cgactcgcgc tccttgaggc gcgggaacat gcggaaggcc cgctccatgt
    12541 cctcggcgat ttccttctta ttctttttga ggtaagcgcc catctggagg ttctcacgca
    12601 cggtgaggtt ggcgaagacg ccgcgcccct ccggcgacat cgagatgccg cgcgcgacga
    12661 gcttgtgcgt cggcgtgccg ctcacgtcgt cgccctcgaa gacgacgcgt ccctcggtcg
    12721 gagagagcag ccccgtgatg gtgcgtagcg tcgtcgtctt ccccgcgccg ttcgcgccga
    12781 tgagcgtcac gacctcgccg cgctcgacgc gcatcgacac gcccttcagc gccttgatcg
    12841 cgccgtaact gacggagacg ttttcgagtg taagcataag gccgggagtc aggagtcagg
    12901 agtcaggagt cagaataaga aaggcgggcg gacggttttt attctgactc ctgacgtctg
    12961 actcctgact tcaatctcct aaataagctt cgatgacctt cgggtcgttc ctgatttctt
    13021 cgggcagccc gagcgcgatg gacttgccgt aatcgacgac ctgaatgcgc tcgcagacgc
    13081 ccatcacgac cttcatgttg tgctcgacga ggaggatggt caactggaag cggtcgcgta
    13141 tctggcggat cagtttgaga aggtcgtcct gctccgaggg gttcatgccg gcggccggct
    13201 cgtcgagcag gaggaggcgc gggcgcgtcg ccagcgcgcg gacgatttcg agccggcgct
    13261 ggttgccgta gggcaaggaa gtgccgccct cgtcgcggaa gcgcgcgagg tcgaagattt
    13321 ccaaaagctc cagacagaac tgctcgtgct cgcgctcgtc gcgtgcgaag cgcggcgtgc
    13381 gcaagacggc gtcggccaaa gtctgctgcg tgtggatgtg gcaggccgtc atcacgttct
    13441 ctaacacgga catgctcgga aagagccgga tgttctgaaa cgtgcgcgag acgccgaggg
    13501 cggcgatctc gtaggggcgc aggccgtcca cgcgcttgtc ctcgaactca atcgtgccgg
    13561 aggtcgggcg atagacgccc gtgagcaggt tgaagatggt cgtcttcccc gccccgttcg
    13621 gcccgatgag gccgtagatt tcgccggggc gcacggccat gtcgagcgcg ttgacggcga
    13681 cgaggccgcc gaagcggatg gtgcactggg ttgttttgag tatcggcgcg ccgaagcccc
    13741 gatccaccat cgccgcggcg gcgtcggtcg cgccgcccgc atgggtcgtc cccgcgttcg
    13801 gtgtcatggc cgcttgtccc cctctccgag cgaagtgtcg cgcgcgcgct cgcccagagt
    13861 cgccttgtca acgtcagtct ggtcgcgcgt gtcctcggcc gtaatctcct cggcctcggt
    13921 gcgagtcttg gcgcggttgc gcgcgcgcca gcccttgaag gggtcgagtc cgccgagcag
    13981 gccgttgggc cgcgtgatca tcagcacgat gagcaggatg gaatagatga ccatgcggta
    14041 ctgcgcgaac tcgcgcagcg cctcgggcag caccgtcagc acgatggccc ccagcactga
    14101 gccgacgatg gagccaagcc cgccgaggat gatcatgatg aggatttcaa acgacttgat
    14161 gaactcgaac gacttcgggt tgaggtagcc gtcgaagtgt gcgaggagca cgccgcccgc
    14221 gcccgcgagc gccgatgaga tgacgaaggc catcaccttg tagcgcgtcg tgttgacgcc
    14281 catcgcctcc gaggccagct cgtcctcgcg gatggagacg agcgcgcggc cgatggccga
    14341 gtgtacgagg ttgtggacga cgacaatcgt cagcaccgcg acgagggcga tccagaagaa
    14401 gctcttgacg atggggatgc cggagtagcc gcgcgcgccg ccgatggcct cgatgttgtt
    14461 gaggaagacg acgatgatct gcccgaagcc gagcgtgacg atggcgaggt agtcgccctt
    14521 aagcctcaaa gacggcacgc cgacgaggaa gccggccgcg cccgcgacga gcgcgccgag
    14581 cagcgtcgcc accaacagcg cagcccccca cccgagcagc ccgtcgccca gcgagcgggc
    14641 catggcggcc ccgtaactca ccgagaagta ggccgaggag taagcgccca ccgccatgaa
    14701 gcccgcgtgc ccgatggaga actgacccgt gaagccgtta atcaggttca gcgagacggc
    14761 gagcgtgatg ttgatgcccg ccagcatgat gatgcgcgcg acgtaggggc tgatcatccg
    14821 ccgccccgga tcgaacacaa aatagtccag cgcgtagagc accgcgagca gcaggaaggc
    14881 gacgacgagg ttacgcgcgt agcgtggcat atgaactcaa gggagtcagg agtcaggagt
    14941 caggagtcag aagtcagaat gggagtcata gtccgcgtcg aataccggac ggtcgaacag
    15001 ttttttattc tgacttctga cttctgactc ctgacttccg ttccatcgtt agactttttc
    15061 ctgcgcgacc gagccgagca ggcccgacgg gcggaagagg agcacgagaa tcagaatggc
    15121 gaaggcgacc gcgtccttgt aggtcggcga aaggtagccg acgacgaggg tctccgtaat
    15181 cccgatgagc aggccgccga gcatcgcgcc ggggatgttg ccgatgccgc ccagcaccgc
    15241 ggcgacgaac gccttgagac cgacgaggat gcccatcagc gggtcgatgc gcgggatgcg
    15301 gatggcgacg agcacgccgg cggccgccgc cagcgccgag ccgagcgcga aggtgaaggc
    15361 gatgatgtag tcggtgttga tgcccatcag cttcgccgac gtgatgttga aggagacggc
    15421 gcgcatcgcc ttccccgtct tcgtgtagta gacgatgtac tgcaagacga ccatcagccc
    15481 gacggcgatg acgagcacga cgatgtccga ggagttgacg gacacgcccc cgccgaggtc
    15541 gatgttctgc ttggtgaaga gttcggggaa gaatttcggg tcggcgccga agacgacctg
    15601 cccgccgttc tccagcagga gcgaaacgcc gatggccgta atcagcgccg tcaggcgcga
    15661 gtacttgcgc acaggccggt aggccaatcg ctcgatgatg acgccgaggg cggcgcagat
    15721 ggccatcgcg aggagcatca cgacgagggc cgtaatcagc gacggctcca acgcgccgct
    15781 gccgagcagg ccgcgcgcga agacgccgtt cgggtcctga ccgagcttct gcgcccactg
    15841 cgcgccgagg ccgttggcga ggtaatagcc catgaaagcg ccgaccatgt acacgtcgcc
    15901 gtgcgcgaag ttgatgagct tcagcacgcc gtagaccatc gtgtagccga gcgcaatcag
    15961 ggaataaatc gccccgagtg agatgccgtt gataagctgc tggatgaacg tactcatgcg
    16021 ttcttgagcc gcgggcgatt gagattggaa gagctttgtc tgaaactgtc ggggcttgct
    16081 aagaactcat gcgggggttt ctaacagggc gttcggcgcc cccgcatccc gtctcggatt
    16141 tgcggtgtgg gccgccgcgc gctccgatgc ctgacggcgg cggcccactc cttctgccac
    16201 ccgacggctc ggccgtcagg gcgtgacgtt ggccgggcgc gcgttggtgt tcgtcgcgcc
    16261 ggcgttcgtg ttgatgttga tgttgatgtt cgcgccggtg tttgcgttcc ccgtcgccat
    16321 gttcgcgttc gacgcggggg ccgcgttcgt gttggtgttg gccgcgccgg cttccgtggg
    16381 catcgacgcg ccctcgacgc tgacgcgttc ctgaatgacg tacttgccgc cctcatcgac
    16441 gcggatcatc atgatcggct tgacggcgtc gcggttggcg ttgaagttca cggtgcccgt
    16501 cacgccgggg aagtccttcg tcgcggcgag ggcgtcgcgg atggccttgc cgtcgagcga
    16561 gcccgcgcgc ttgatggcgt cgaacatgat gagcgccgcg tcgtaggcgg tggcggccag
    16621 cgcgtcggga atccccccgt aacgctgctg gtagccctgc acgaacttct gcaccttcgc
    16681 gtcggtgtcg tagggcgagt agtgattggt gaagtagctg ccgacgacgg ccgcgccgcc
    16741 gatggtgtag agctgcgccg agtcccagcc gtcgccgccg acgagcggca cgttgaggcc
    16801 cttgtcgcgc gcctgcttgg cgatgagcgc cacgtcgccg taatagccgg gaatgaaaat
    16861 cacatcgggg ttagcgccct tgatcgaggt gagctggccg ttgaagtcag tgtcgccttc
    16921 ctggaacgac tgcgtgatga cgacctctcc gccgaaacgc gggaacactt cgttgatgac
    16981 tttttcgagg ccggtcgagt agtcgttctt gcggtcaacc atgagggccg cgcgccgctt
    17041 gccgaggctc ttgccggcga actgcgacat ggccgcgccc tgcacggggt cgataaagca
    17101 actgcggaag atgtagtcgc cggccttggt cacgtcgggg ttggtcgaag agggcgtgag
    17161 catcggtatc ttcgcgttct gcgcgatggg cgcggcggcg atggagcgcg aagaggccac
    17221 ctcgccgaga actgcgtgga cgccgtcctg cgtgacgaac ttggtgacga tggtcgcggc
    17281 ctcctgcgcg tcggagcggt cgtcctgcac gagcagctcg atctgcttgc cgttgatgcc
    17341 gccggcggcg ttgacttcgt cggcggccat cttgatgccg ttagtcgaag agatgccaaa
    17401 ctgcgccgtg ctccccgtca gcgacatgaa gacgccgatt ttgactttgt cgccgcccgc
    17461 cgtgccgccg ccggggccgc cggtctggca ggccgtcgcg gccagcatcg aagccgtcaa
    17521 tccaagtgcg attaaggtgc gtttcatatt ttcctcgtcc agtgtgttgc gtaaaggtgc
    17581 ggcgcatgcg tcggcgcgcc ggtgtatcgt tcggacaagc ctcgccacgg acaccgcgcc
    17641 gcctcacctg ccgaaagtct agctgcccga accgaaccct gttgaagtgg ggggttgact
    17701 gctcgcgtac cgtaatatcg cgacacaccg cgggcggagt caaggggccg ccgagtgatt
    17761 tttacaattc gacgcgcatt atacattttt gacgcgccgt gtcgagtatc ttccacaagc
    17821 ggcccgcgcg cggggcgcga gaacggttca acagcgggac acggatgaga cggattaaaa
    17881 cggatcgtca cggatttaat ccgtcctcat ccgtcctcat ccgtctcatc cgtgttccat
    17941 ttctcccgcc tgttccgcct cgcgcgctct cgtgtcatac tcacctgact tttacaccgc
    18001 atcctcgcga gcgctgtcag aggcgaacac gccgacacga gacgagtcga atccttatga
    18061 agcagacaga gagtgagacg ggcggcctcg ccccgccgct cggcgacatg agcgccgagg
    18121 agttccgacg ccacggccac gagctggtcg aatggatcgc cggcttcctc gaacgcctcg
    18181 aagacttccc cgtgctcgcg caggtcaggc ccggcgatct caaatctcaa ctcccacagt
    18241 cgccccccgc cgagggcgag ccgatgccgg agattctggc cgacgtggaa cggctcgtcc
    18301 tgcccgcgct cacacactgg aaccaccctt cgttcttcgc ctacttcgcc acctcttcgt
    18361 ccgcgcccgg catcttcggc gaactcctct cggccgtctt cgacgtgaag tcgatgctct
    18421 ggcgcacctc gcccgcctcg accgaactcg aagaggtcac cctctcatgg ctccggcaga
    18481 tgatgggcct gcccgccgag ttcgacggcc tcatctacga cacggcctcg gtcgccacca
    18541 tgcacgccgt cgccgccgcg cgcgaggcgc tcgacatgcg cgtgcgcgag gacggcatga
    18601 gcggacgcgc cgacctgccg ctgctgcgtc tctactcatc cgagcaggcc cactcctccg
    18661 tcgagaaggc gctgctcgcc ctcggcctcg gccagcgttc gctctgcaag gtcgccgccg
    18721 actccgagtt tcgcatgaag cccgacgcgt tggccgaagc catcgacgaa gaccgacgcg
    18781 ccggcatgct tcccttttgc gtcgtcgcca ccgtcggcac gacctcgaca tcgagcatcg
    18841 accccgtggc ggagatcgcc gacgtgtgcg aacgcgagcg actgtggctg cacgtcgatg
    18901 ccgcttatgc cggctcggcg gcggtcgtcc ccgagttgcg accattgttc gcgggctgtg
    18961 agcgcgccga ctccttcgtc gtcaacccac acaagtggct gtttacgccc ttcgacctct
    19021 cggccctcta cacgcgccgc ctcgacatgc tgcgccgcgc cttcaccctc acgcccgagt
    19081 atcttcgcac gcccgaggcc gagaccgtgc gtcacggctc ggactacggc gtccagctcg
    19141 gccgacgctt ccgcgccctc aagctctgga tgattctgcg ctacttcggc cacgacggac
    19201 tcgccgcgcg catccgcgag cactgtcgcc tcgcgcgcct cttcgcctcg tgggtcgccg
    19261 agacgcccgg ctgggaactg ctcgcacccg tccccttcgg cctcgtctgc ttccgcgcct
    19321 gccccgtcgc ggacgacgag accgaagcgg cgcgcggcgc tcggctcgac gctctcaacg
    19381 agtcgttgat gaactccgtc aatgctaccg gccagatgtt tctctcacac accaagctga
    19441 acgatgtctt caccctgcgt ctctccgtcg gcaacatccg caccacggag gcgcacgtcc
    19501 gccgcgcgtg ggagttgctg aacgaacacg ccgcgcgcct cgcggattcg ccatcgtccg
    19561 tgtaatctcg gccatacatc cgtcgcgctt cagcgaggcc gtatgcaaca agtttccaac
    19621 atgcgaagca cgctcgccat ccttgccctg cttgcgtcgc tcaccgtcgc ggtttcactc
    19681 aaggcgcaga cgcggcgcgg gatgacgccc gaggacacgt tgcgcttcgc cgaagtgggc
    19741 gacgcgcaaa tctcgcccag cggcgagtgg gtcgtctacg tcgtcgccac gaccgagggc
    19801 gaggagcggc gctcggccct gtggctggtg cgcgcggggc cgggggccgt caccagcgtc
    19861 cccggcttcg tgcaggagtc gcccgacgcc tcgcgcagcg tcatcgcccc cacgcggatc
    19921 aacacggcgg ggctgaacgc ctcgcggccg cgctggtcgc cggacggcca ccgcctcgcc
    19981 ttcgtcggtt caggcggggg ccgcccgtcg ggcgtgtggg tcgtctcctc cttcgagcgc
    20041 gagtcgcagc cgcgcctcgt cgccgtcgtt cgcgacagca acttccacgt cccttacgcc
    20101 ggcgagtcgt tcgcctggtc gcccgactcc agacgcatcg cgtacatcac ggcgacggaa
    20161 gaggccgatg acggggagcg gcgaccgacg gttgcgggcg cgcgcaacga cgacccgcga
    20221 gtcttgacgc gcgtccgcta caagtcgcgc acctctttct ccgacacgct gcgcacgcac
    20281 gtctggctcg ccgaggtgga cgaccccgcc gcgcagccgc ggcagctcac ctccggcccc
    20341 ttccacgatc acgccgtctc ctttaaccca cgcgccgacg agatcgcctt cctctccaac
    20401 cgcgagcctg accccgacgc cgttaacaac tccgacatct acgccgtctc gaccgacggc
    20461 cgcacgcgcc gtgtcacgga aacgcgcggg tgcgagtacg acccgcaatg gtcgcccgat
    20521 ggacgcctca tcgcccacac ggcgacgacg cgcgacgtga cgaccatcga cagcgtcagc
    20581 gaagacgcgc acgtctgggt cgtcgaatcc ggcggcggcg ggcgacgtga aatcagcgcc
    20641 gccctcgacc gtcgcgcgcg ctcgccccgc tggtccgcgg acggccgctc cgtcctcttc
    20701 ctcgccggcg acgcggggcg cacgctcctc ttccgcgccc ccatcagcgg cggccccgtc
    20761 gcgcgcattc acttcgaccg cgcggacgac agtccgctcc gcaacctttt cgcgtcgccc
    20821 ggcgcgcccc agccgcagcc gtttcaactc ggcaacttca gcgtgagcgc gcgccgcccg
    20881 tcgctcttcg ccttcacgct gagcaccccg ctccacccgt cggaggtctg ggccggcgag
    20941 tcaactgaag gcgacgcccg ccgactcagc gcacacaacg acgtgctcct gcgcacgctc
    21001 tcgctctccg cccccgaggt cttctccgtc cgcagcttcg acggagcgca ggttcaggcg
    21061 tggctcatgc ggcccgccgc ctttcgccct tcgagcggcc agcgccaccc cgtagtgtta
    21121 agcgtccacg gcggcccgca cggcgcctac ggctacggct tcaacgccga gtttcagacc
    21181 tacgccgcgc gcggctacgc cgtgctctac atgaacccgc gcggctccac cggctacggg
    21241 cagaagttct ccgacggcac cttccgcgag tggggcggcg gcgactatcg cgatcttttg
    21301 gcgggcgtgg acgaggcgct gagtaaatat tcgtggctcg acccggagcg catgggcgtg
    21361 gtcggcggca gctacggcgg cttcatggtc aactggatca tcacgcagac gccgcgcttc
    21421 cgcgccgccg tcgcccgcgc cagcgtcagc aacctcgtca gcttttacgg cacctcgctc
    21481 tatcaggatt tgattcaagc cgagttcggc cagatgccgt gggacgacta cgacttgctc
    21541 tggcgctggt cgccgctccg ctatgtccgc gacgcgcgca cgcccacact cttcatccac
    21601 ggcgaactcg acaacgacgt acacatcaca caggccgagg agatgtacac ggccctgcgc
    21661 cgccgcggcg tcgaggccgc gctcgtgcgc tacccgcgcg agggccacgg cttccgcgag
    21721 ccgcgccacc gccgcgactc catcgagcgg gcgctcgact ggttcgacag gtttttgaag
    21781 tgaaggaaag ggagggagtc aggagtcaga agtcagaagt cagaatgaaa gttagcgacc
    21841 ggcgttctta ttccgacttc tgacttctga ctcctgactc ccttgcagag gttttatgct
    21901 gacacacatc gtcatctgga aatatcgcga ggacgtgggg gaagaggagc gtcgtctgca
    21961 cgtcgagatg ctgcggcggc tccccgttct catcccggag gtcttgagtt tccacgtcgg
    22021 gttcgacgtg ctgcgcctgc cgcgctccta ccacaccgga ctcgcctcga ccttccgcga
    22081 ccgccgcggg cttgaggctt acaccgtcca tcccgagcac gtcgccgtgg ccgacttcgg
    22141 ccgccgcatc agcgagcacg tggcctccgt ggacttcgag gaggataaag acgtaaatcg
    22201 taaatcgtaa atcgtggttg agcgactttc aggtggcgcg ttccgcaggc gagccagcgc
    22261 agcgtcaacc cattacgatt tacgattcac gattcacgat ttacggtttt ttgactgatg
    22321 aagcgttgga agaagatcgc gctcgtggtt gtgggcctgc tgattctttc gcaggcgccc
    22381 ttcgtctacc ggcgctaccg gctgggacgg ctccgcgcgg ccatcgactc cgtcaacgcc
    22441 tcgcgcgctg ccgctgtggc cgacgacccc ttcgtcgatt acaaaggcgt cctccacgtc
    22501 cactcctccc tcggcggcca cagcaccggc acgctcgacg agatcgtagc ggcggcgcgc
    22561 gcgaacgaac tcgcgttcgt cgtgatgacc gagcacccgt cgaagtacgt ggacacggtc
    22621 gcggcgacac tcaagggtac gcatgaaggc gtcctcttca tcaacggcag cgagctgagc
    22681 gccgcaaacg atgagcgcct gttcgtcgtg cccggcccct ccgtgccggc cgccggcccc
    22741 gccgcgccga acgcgcaggc gctcgtcgag cgcgcccgcg ccgaaggccg cctcgccctc
    22801 gtcgcctacc ccgagaggat tcgcgactgg ggactgctgc aaggctacga cggcatcgag
    22861 gtctacaacc tctacaccaa ctccaaacgc atccgttacg gcctgctctt cttcgacggt
    22921 ctctggtctt accggagcca cgccgacctc ctgttctcga ccttctacga gaggccggac
    22981 gagaacctgc gccgttggga cgagctgacg gccgcgcgcg gcctccgcct gtcggccgtc
    23041 gccggcaacg acgcccactc caacgtcggc ctccgcctcg gcgaccgcac gggcaaggag
    23101 ttcttaggcg tcagcctcga cccctacgag cgcagcttcc gcgtcgtccg caaccatgtg
    23161 ctcctcgaca gggcgcagcc gctcgccg
//
LOCUS       DASVES010000438         6589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1552996, whole genome shotgun sequence.
ACCESSION   DASVES010000438 DASVES010000000
VERSION     DASVES010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552996"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VF240_18015"
     CDS             <1..1504
                     /locus_tag="VF240_18015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7177160.1"
                     /translation="IEFPYPVAPVVRHHHENWDGSGYPDGLKGEEIPLGARILSVVDC
                     FDSVREDRPYRKGMTTEEAGALLRRGAGTHFDPRVVSLFLEHLPEFEREVAAEGLDQH
                     GVTSVECDARTLLMDADASMTLLEAPAARVHGNNNAPAPAYLHHINNAHREVYALYEI
                     ARTFGSSLDIENTVSVLVNKVGHVVPFDLCAVYLHDELKGYATAAHVAGPNSDLVRGR
                     AVAPGEGVIGFVLANRRPAHHLDPALDFTDLLLPAGVEYRSMVALPLVRDEQLLGALA
                     VYSAEPGRYTDDHVRMLDTVARLASDALANAVQHAEAESNALTDNLTGLPNARAMFAR
                     FEQESARARRTGRPFQVIMLDLDDFKQVNDRFGHKAGDQMLREVARLLQAQLREYDFL
                     ARYAGDEFVALVQELTAEQSEELSVRIERVVSQFSLHVRGDQRARVGISVGSATYGLH
                     GETLDQILIAADEAMYHIKSYHKQQARAAASSADTHSSLNTGDLTSTAVN"
     gene            complement(1697..2509)
                     /locus_tag="VF240_18020"
     CDS             complement(1697..2509)
                     /locus_tag="VF240_18020"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7177161.1"
                     /translation="MPNPRDYQFNLPASDRPSGVTIPEQWYPGVQNYWPRCTSQRIVG
                     DHINGVVAVVIHATAGFRSADAVSVMHPPPGDSAASFHWLVPDEDEPQHGQLIWACVR
                     ERDAAWHVRPGASHADVNGGRNRVNHWSLGVEIVNRQLGGDAFSDWQVAIVAQLVRYC
                     WSKYPNLRHVVSHAKLDPLRRTDPGANFPWERFKGLVLNPPDDASPNLVTADVASSTS
                     RNIIETQETASLEEEEANFNREESEAYSGAAPAGADSGAGTNADKLHEAQDG"
     gene            2899..4983
                     /locus_tag="VF240_18025"
     CDS             2899..4983
                     /locus_tag="VF240_18025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177162.1"
                     /translation="MPAPATNPGQKATLLIVESDAQARARLAGAFEAEGYRVVACDDA
                     PSALRRLRHERFDLAVLDAELPGVGGLALCRLLRAQDATRRLAVVVASSRGDEGHRVE
                     AFAAGADDFVLKSSASRELLTRVRAHLESAARQRALEGTNRELAFLADLGRGLLHAMS
                     PAEVVRRVAGAAYEGANASMCAAVLTKPEGDKTRVGQGVVICVFDREGSAEEDISFVD
                     IWRLRAWLDSAKAAPARLGEQSQFFLRDDRHVVEYAAPLRFEGRALGALVAAFDHPAD
                     CGENEERLLDAAAQQAALAAHISSLYEAARESSKVLAKEVERRTAEAESQRRFTEAII
                     DSLPVSLYAVDRKYRVVAWNRNRETGGQGIPRGSAIGRNVFEVLTRQKRGVLEDEFER
                     AFRTGSIERIEQETTSADGSTRHWLVSKVPMRIDGAEVSHVITVGEDVSARVEANRAV
                     ARTEKLAAVGRLAAGVVHEINNPLATIAACAEALESRVAEGAYGASPEVEDLREYLQL
                     IRSETFRCKSITNGLLDFSRARRAGERAPVNVAEVIESAARLLMHQKRGAAVRIDVEA
                     ERGLPLVMGDEGQLQQAVIILAENAIDAMSDGGTLTLRARPADDGTRAVLLDVGDTGA
                     GISPENVAKIFDPFFTTKEVGRGTGLGLAVCYGIVTEHGGRVSVDSTVGRGTTFTLTL
                     PATSNEDGDSES"
     gene            4980..6389
                     /locus_tag="VF240_18030"
     CDS             4980..6389
                     /locus_tag="VF240_18030"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7177163.1"
                     /translation="MSETQQAKILVAEDEANLRLVLQKELQRMGHDVRVAPDGEAALK
                     LLEESAADVLLCDINMPRMDGMELLRRVRQRPNPPEVVMLTGRASVETAIESMKLGAY
                     DYLSKPYHINELDALIKQAAEKRRLRVDNQRLRALAERQSAMPEIVSVSDAMRESMRL
                     VERVAPADTSVLITGESGTGKELIAHAIHRLSSRSEGSFIDLNCAAIQETLLESELFG
                     HEAGAFTNAKSRKLGLWELADNGTIFLDEVTELPPQLQSKLLRAIETSSFYRVGAVRK
                     VQVNVRIIAATNRDVNEVVGDGRFRADLLYRVNGFQIDLKPLRERPEDIEPIARHLMK
                     KLGGTQPPELTPEVLEALKSYAWPGNVRQLRNTLERATLLANDGRITVAELPPEIVAP
                     ARTFIPVSVSSTGAGDSAGGGAQTQGGVPPLREVERQQILAALEQTNWHRGKTAELLG
                     ISPSTLYRRLRDYNLMRRP"
BASE COUNT         1075 a         2303 c         2153 g         1058 t
ORIGIN      
        1 gatcgagttt ccgtatcccg tcgcgccggt cgtccggcac caccacgaga actgggacgg
       61 ctccggctat cccgacggcc tcaaggggga agagattccc ctcggcgcgc gcatcctctc
      121 cgtcgtcgat tgcttcgact ccgtgcgcga agaccggccc tatcgcaagg ggatgacgac
      181 cgaggaggcg ggcgcgctgc tccgacgcgg ggccggcaca cacttcgacc cgcgcgtcgt
      241 ctcgctcttc ctcgaacatc ttcccgagtt cgagcgcgag gtcgccgcgg aaggtctcga
      301 tcagcacggc gtgacgagcg tcgagtgcga cgcgcgcacg ctcctcatgg atgcggacgc
      361 ttccatgacg ctcctcgaag cgccggccgc gcgcgtccac ggcaacaata acgcgcccgc
      421 gcccgcctac ctccatcaca tcaacaacgc ccaccgcgag gtctacgcgc tctacgagat
      481 cgcccgcacc ttcggctcgt cgctcgacat cgaaaacacc gtctccgtcc tcgtcaacaa
      541 ggtcggccac gtcgtcccct tcgacctgtg cgccgtctac ctccacgacg agctgaaggg
      601 ctacgcgacg gccgcgcacg tcgcggggcc aaactccgac ctcgtccgcg gccgcgccgt
      661 cgcccccggc gagggcgtca tcggcttcgt gctcgccaac cgccgccccg cgcatcacct
      721 cgaccccgcg ctcgacttta cggacttgct cctgccggcc ggcgtcgagt accgctcgat
      781 ggtcgccctc ccgctcgtgc gcgacgagca actgctaggg gccttggccg tctactccgc
      841 cgagccgggc cgctacacgg acgaccacgt ccgcatgctc gacacggtgg cgcgtctcgc
      901 ctcggacgcg ctcgccaacg ccgtgcagca cgccgaagcc gagtcgaacg ccttgaccga
      961 caacctgacg gggctgccca acgcgcgcgc catgttcgcc cgcttcgagc aggagtcggc
     1021 gcgcgcgcgc cgcacgggcc gtcccttcca ggtcatcatg ctcgacctcg acgacttcaa
     1081 gcaggtcaac gaccgcttcg gccacaaggc cggcgaccag atgctgcgcg aggtggcgcg
     1141 cctcctccag gcgcaactgc gcgagtacga cttcctcgcg cgctacgccg gcgacgagtt
     1201 cgtcgctctc gtccaggagt tgacggccga gcagagcgag gagttgagcg tgcgcatcga
     1261 gcgcgtcgtc agccagttct ccctacacgt gcgcggcgac cagcgcgccc gcgtcggcat
     1321 ctcggtcggc tcggccacct acggtctcca cggtgagacg ctcgaccaga tactgatcgc
     1381 cgccgacgag gccatgtacc acatcaagtc gtatcacaag cagcaggccc gcgccgctgc
     1441 ttcttcggcc gacacccact cctccctcaa caccggcgac ctcacctcga cggctgtcaa
     1501 ctaagccaag tttcagtcgg acgcgttaca cgggtgatgt cagtaggggc agggctcgtt
     1561 cctgcccgca gtcgcaactc attcaacttt gagtgaattg taaccgcggg cagggaacga
     1621 tccctgcccc tacagtcgtc ccggttttct tagcggctct cagccggttg aggtctcggt
     1681 ctgtcgaaga gcgcgcttaa ccgtcctgtg cctcgtgcag tttgtcggcg ttcgtgcccg
     1741 cgcccgagtc agctccagca ggggcggctc ccgagtatgc ctctgactct tcccggttga
     1801 agttggcctc ttcctcttcc agagacgcgg tctcctgcgt ctcgatgatg ttgcgggagg
     1861 tcgaactcgc cacgtcggcg gtgaccaaat tgggtgacgc gtcgtcgggg ggattcagga
     1921 ccaagccctt gaatcgctcc caagggaaat tcgcgcccgg gtcggtgcgc cgcagcgggt
     1981 ccagtttggc gtgcgagacg acgtgtcgca ggttcgggta cttcgaccag caatagcgga
     2041 cgagctgcgc cacgatggcg acttgccagt cggaaaacgc gtcgccgccc agttgccggt
     2101 tgacgatttc aacgccgagc gaccagtggt tgactcggtt ccggccgccg ttcacgtccg
     2161 cgtggctcgc ccccggcctg acgtgccaag cggcatccct ctccctcacg caggcccaga
     2221 tgagttggcc gtgctgcggc tcgtcctcgt cgggcacgag ccagtgaaag ctggccgcgg
     2281 aatcgccggg cggcgggtgc atcactgata ccgcgtctgc cgagcggaag ccggccgtgg
     2341 cgtgaatgac gaccgccacc acgccgttaa tgtggtcgcc gacaatccgc tgcgaggtgc
     2401 aacgcggcca gtagttttga acgccgggat accactgctc ggggattgtc acccctgacg
     2461 gcctgtccga tgcgggcaaa ttgaactgat agtctcttgg gttaggcatc gcgaagactc
     2521 ctgtgaaatt aacgttagtg ggtcaatttc aattccgaca agaccgctcg gcaatcgaac
     2581 tgccccaggc aatcactctc aacactattg cctacatctt tatgcgctct cccggcaaga
     2641 catttcatga ggccgcgtgg gcacggctga aaatatagct tttaagttca aactgaccca
     2701 ctaccgcccc gcagcttttc ttgactttcc ccgccccatc gctttcaatc gaatgtaagc
     2761 gccggggtgc ggccggcgcc gcttcgtcac gtccgcccac aggtcggcgc cgcgggcgcc
     2821 gcgaaacagc ttcagcgggt cttaatcttt tctcaccgca ccccgccaac aaccccgaga
     2881 acgactgtaa agagatcaat gccggcccct gccacgaatc ccggtcagaa ggccacgctg
     2941 ctcatcgtcg agtcggacgc gcaggctcgc gcgcgtcttg cgggcgcgtt cgaggccgaa
     3001 ggctaccgcg tcgtcgcctg cgacgacgcg ccctcggccc tgcgccggct ccgacacgag
     3061 cgcttcgacc tcgccgtgct cgacgccgaa ctgcccggcg tcggcggact cgccctgtgt
     3121 cgtctgctgc gcgcgcagga cgcgacgcgc cgcctcgccg tggtcgtcgc ttcctcgcgc
     3181 ggcgatgagg gacaccgcgt cgaagccttc gccgccgggg ccgacgattt cgtcctgaag
     3241 tcgtcggcct cgcgtgagct tctgacgcgc gtgcgcgcgc acctggagtc ggccgcgcgg
     3301 cagcgcgcgc tcgaaggcac caaccgcgag ctggccttcc tcgccgatct ggggcgcggg
     3361 ctgttgcacg cgatgagtcc ggccgaggtg gtgcgccgcg tggcgggcgc cgcctacgaa
     3421 ggcgccaacg cctcgatgtg cgcggcggtg ctgacgaagc ccgagggcga caagacgcgc
     3481 gtcgggcagg gagtcgtcat ctgcgtcttc gaccgcgagg gaagcgccga ggaggacatc
     3541 tcgttcgtgg acatctggcg gctgcgcgcg tggctcgact cggcgaaggc ggcccccgcg
     3601 cggctcggcg agcagtcgca gttctttttg cgcgacgacc gccacgtggt cgagtacgcc
     3661 gccccgctcc gcttcgaggg ccgcgcgctg ggcgcgctcg tcgccgcctt cgaccacccg
     3721 gccgactgcg gcgagaacga ggagcgcctc ctcgacgccg ccgcgcagca ggccgcgctg
     3781 gccgcgcaca tctcctcgct ctacgaggcg gcgcgtgagt cgtcgaaagt tcttgcgaag
     3841 gaggtggagc gccggacggc cgaggccgag agccagcggc gcttcaccga ggcaatcatc
     3901 gactctctgc cggtctcgct ctacgcggtg gatcgcaaat accgcgtggt cgcgtggaac
     3961 cgcaaccgcg agacgggcgg gcagggcatc ccgcgcggaa gcgccatcgg ccgcaacgtc
     4021 ttcgaggtct tgacgcgcca gaagcgcggc gtgttggagg acgagttcga gcgcgcgttc
     4081 cgcacgggga gcatcgagcg catcgagcag gagaccacgt cggccgacgg ctcgacgcgc
     4141 cactggctcg tcagcaaggt gccgatgcgg atcgacggcg cggaggtctc gcacgtcatc
     4201 accgtaggcg aggacgtgtc ggcccgcgtc gaggctaacc gtgcggtcgc gcgcacggag
     4261 aagctggcgg cggtggggcg cttagccgcc ggcgtcgtcc atgagatcaa caacccgctg
     4321 gcgaccatcg cggcctgcgc ggaggctttg gagtcgcgcg tcgcggaggg cgcttacggc
     4381 gcgtcgccgg aggtcgaaga cctgcgcgag tacttgcagc tcatccgtag cgagactttc
     4441 cgctgcaagt cgatcacgaa cggactgctc gacttcagcc gcgcgcgccg ggccggtgag
     4501 cgcgcgcccg tcaacgtcgc cgaggtcatc gagtcggcgg cgcgtctgct catgcaccag
     4561 aagcggggcg cggccgtgag gatcgacgtt gaggccgagc gtggcctgcc gctcgtcatg
     4621 ggcgacgagg gccagctcca gcaggccgtc atcatcctcg ccgagaacgc catcgacgcg
     4681 atgtccgacg gcggcacgct caccctgcgc gcgcgcccgg ccgacgacgg cacgcgcgcc
     4741 gtcctgctcg acgtgggcga cacgggcgcg gggatttcgc cggagaacgt ggcgaaaatc
     4801 ttcgacccgt tcttcacgac gaaagaggtc gggcgcggga cggggttagg gctggcggtc
     4861 tgttatggta ttgttaccga gcacggcggg cgcgtcagcg tcgattcgac ggtggggcgc
     4921 ggcacgacct tcactctgac gctgccggct acttcaaacg aagacggaga ctccgaatct
     4981 tgagcgaaac ccaacaggca aagatcctcg tcgcggagga cgaggccaac cttcgtctgg
     5041 tgctccagaa agaactccag cgcatgggcc acgacgtgag agtcgccccc gacggcgagg
     5101 ccgcgctcaa gctcctcgaa gagtcggccg ccgacgtgct cctgtgcgac atcaacatgc
     5161 ctcgcatgga cggcatggag ctgctgcgcc gcgtgcgcca gcgcccgaac ccgcccgagg
     5221 tcgtcatgct gacggggcgg gccagtgtcg agacggccat cgagtcgatg aagctcggcg
     5281 cttacgacta cctctcgaag ccctaccaca tcaacgaact cgacgcgctc atcaagcagg
     5341 ccgccgagaa gcgccggctg cgggtggaca accagcgtct gcgcgcgctg gccgagcggc
     5401 agtcggcgat gcccgagatc gtcagcgtct cggacgccat gcgcgagtcg atgcgtctcg
     5461 tcgagcgcgt ggcgccggcc gacacgtcgg tcctgatcac gggcgagtcg ggcacgggga
     5521 aggaactcat cgcgcacgcc atccaccgcc tatcgagccg gagcgaaggg tcgttcatcg
     5581 acctcaactg cgcggccatt caggagacgc tgctcgaatc cgagctgttc ggccacgagg
     5641 ccggcgcgtt cacgaacgcg aagtcgcgca agttgggtct gtgggagctg gccgacaacg
     5701 gcacgatctt tttagacgag gtgacggagc tgccgccgca gttgcagtcg aagcttctgc
     5761 gggcgattga gacgagttcg ttctaccgcg tcggcgccgt ccgcaaggtg caggtcaacg
     5821 tccgcatcat cgccgcgacg aaccgcgacg tgaacgaagt ggtcggcgac ggccgcttcc
     5881 gcgccgacct cctctaccgc gtcaacggct tccagatcga cctcaaaccc ctgcgcgagc
     5941 gacccgaaga catcgagcca atcgcccgcc acctgatgaa gaagctcggc ggaacgcagc
     6001 cgccggaact cacgcccgaa gtgctcgaag ctctgaagtc atacgcgtgg cccggcaacg
     6061 tccgccagct ccgcaacacg ctggagcgcg cgacccttct ggccaacgac gggcgcatca
     6121 ccgtcgccga gctgccgccc gagatcgtcg ccccggcgcg caccttcatc cccgtctcgg
     6181 tctcctcgac gggcgcgggc gactctgccg gcggcggcgc acagacacag ggcggggtgc
     6241 cgccgctgcg cgaggtcgag cgtcagcaaa ttctcgccgc gctcgaacag accaactggc
     6301 accgcggcaa gacggccgaa ctcctcggca tctccccctc gacgctctac cgccgcctgc
     6361 gcgattacaa tctcatgcgc cggccgtgaa cagagcgcag ccgcttacag cggctgcgtg
     6421 atgcttagcc ggtctagatt attagccccc gccgccggtg gcggtcttct gcttgttggg
     6481 cggctggccg agggtgcggc gggcggcctc ggcctcgcgg gtgcgccccg tgcgctcgta
     6541 gatggcgatg atgcgttcga ggagggcggg ggagacgggc cgaagtttg
//
LOCUS       DASVES010000439         7842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1053158, whole genome shotgun sequence.
ACCESSION   DASVES010000439 DASVES010000000
VERSION     DASVES010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053158"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VF240_18035"
     CDS             complement(<1..1257)
                     /locus_tag="VF240_18035"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177164.1"
                     /translation="MGKNSHKTDRLWVRLHTMQRKQTMMDGNDAASHVAYKTNEVIAI
                     YPITPSSNMGENADAWAAEGRQNIWGVVPVVREMQSEGGAAGAVHGTLQTGSLTTTFT
                     ASQGLLLMIPNMFKIAGEQTATVFHIAARAVATHALSIFGDHSDVMTTRSCGWAMLFA
                     NSVQEVMDFALVSQAATLRARVPFLHVFDGFRTSHEVQKIEPLTDEDMRALIDPELVR
                     AHRGRALTPDRPVMRGTAQNPDVFFQAREASNPFYLATPSIVQETMDKLAALTGRQYR
                     LFDYVGHPQAERVVVLMGSGCEAAEETVEALLARGERVGLVKVRLYRPFSARDLLSAI
                     PRTARAVAVLDRTKEPGAAGEPLYQDVLTAVGESLMDDASPFVAAPRVGGGRYGLASK
                     EFTPAMAKAVFDELKKSAPKNHFTVGI"
     gene            1474..3399
                     /locus_tag="VF240_18040"
     CDS             1474..3399
                     /locus_tag="VF240_18040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7177165.1"
                     /translation="MTVKQNWQAQYREKLKDAARAVRAVRRGQKVFIGSGAAEPQALV
                     RALSDRAEELADTEIYHIMTLGIAPYAESKFARHFRHNAFFIGTNVRAAIEEGRADYT
                     PVFLSEVPKLFRTRRIPLDVALISVSPPDEHGYCSYGVSVDVVKAAAESATLVIAEVN
                     RQMPRTLGDSFIHVSRIDCLVESDAPLPVLAQGRPDDVAMEIGRHVASLIDDGSTIQM
                     GIGVIPNAVLAQLGGKRRLGVHTEMFSDGVIDLIESGVIDCSKKTLLAGKVVSSFCMG
                     TPRLYEYVDNNPLFEFRSVEFVNDPFQIARNEKMVAINAAIEVDLTGQVAADSIGSRF
                     YSGIGGQVDFIRGAARSEGGKPVIALRSTAKGDTLSRIVPRLQGAGVVTTRGDVHYIV
                     TEYGVADLWGRNVRERAMALINIAHPRFRQELLAEAKRARLVYDDQILLHTASYPKQW
                     ERIEPSKDGTSVYLRAVKPTDEAMMKELFYTCSEQSLYDRYFQVVKAMPHEKLQRIVN
                     VDYENEMSVVARMREGGHERIVGAASYTLNPATNTADVAFLVHDEYQARGLGTLLLQH
                     LMRIARSKGVAGFTADVLAHNHAMLKVFHNSGCEMKSSLADNVYQLEFYFDRAQKGSG
                     AGERDAAEAADSSAASL"
     gene            3450..4166
                     /locus_tag="VF240_18045"
     CDS             3450..4166
                     /locus_tag="VF240_18045"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX7177166.1"
                     /translation="MVEHGHSPEAIRERLAEGPRHSYLRDWVYGGIDGAVTTFAVVSG
                     VIGAQLPPAVILVVGAANILADGFSMAASNYLGTKTELEEREYLRRVEHRHIEDFPEG
                     EREEVRQIFAQKGFEGEELERVVELITSDHARWVNTMLTEEYGLAPEVRSPVRAAAAT
                     FSAFMVCGVAPLLPFVLAAPRAFTLSAVLTGAAFFAVGSAKSFWSPTPWWRSGLTTLL
                     IGAVAACLAYVVGVLTRGLL"
     gene            complement(4169..4375)
                     /locus_tag="VF240_18050"
     CDS             complement(4169..4375)
                     /locus_tag="VF240_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177167.1"
                     /translation="MAERDSTHGELLFSDVPGDREKVRVILEQWGVDGALIRRLMREH
                     AADPHGGVATRVEGKTPPPSHAGY"
     gene            complement(4408..4935)
                     /locus_tag="VF240_18055"
     CDS             complement(4408..4935)
                     /locus_tag="VF240_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177168.1"
                     /translation="MFIATTDRTYLRMLAELVLLALAIFAFGALLVHAQTPSQSGPAT
                     AQVGINTSTTDEPSLREYKGVKLGMSAEEARRRLGEPADKGDEQDFYNFSDTESAQIF
                     YDPARKVMAVSVQFLGGGSGVPTPKSLFGADVTPRADGTVYKLVKYQRAGYWVSYSRA
                     AGDAPLVTVSMQKTR"
     gene            5201..5893
                     /locus_tag="VF240_18060"
     CDS             5201..5893
                     /locus_tag="VF240_18060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7177169.1"
                     /translation="MQIDKVAALRNTLLFGELDEKTLRALAGRAVERRYGRDEVLFLA
                     GEEARGLYCIVAGAVRAFRESLDGREQVIHVERSGATVAELPVFDDGPYPSTVAAEEE
                     TTVLFIDKRDVRRLSLEHPEIALAALKVLAGRLRRCAELVETLSLREVGQRLARFLLA
                     EARTKGAPAGGGLRVELTHSNQQIAARVGSVREVVSRALTRLQHDGLVRLEGRRLTIP
                     DEGALASFAGEE"
     gene            complement(6146..6874)
                     /gene="ric"
                     /locus_tag="VF240_18065"
     CDS             complement(6146..6874)
                     /gene="ric"
                     /locus_tag="VF240_18065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEX7177170.1"
                     /translation="MTLCTEKTVREYAVETPNATRVFEKLKIDYCCGGGRSLGDACAV
                     AGVPVEEVTRLIEQAGSAPAEMPAEIVSGTLAELIDHILDKHHTFTRDEMERITALTE
                     KVCSKHGENHPELLGVRTLFQQLCDDLRPHMFKEEMILFPYVKQMEQAAAAGRPLPYA
                     PFGTVGNPIRVMMNEHDTAGDILRELRATTADYAAPADACISYQTLYHALEELERDLH
                     QHIHLENNVLFPRAVKLEEGMGSK"
     gene            7244..>7842
                     /locus_tag="VF240_18070"
     CDS             7244..>7842
                     /locus_tag="VF240_18070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7177171.1"
                     /translation="MRDIFMIATENELLKKYDVPGPRYTSYPTILHWDNTPTPEEWTN
                     SVARGLDEAEALGEGAAVYVHIPFCRSLCTYCGCNSRITQSKTVGDPYVDAVLREWAL
                     YLDALKRTNSLPLAELHLGGGTPTFLSPAELERLIGGILGGVRRTEDSEFSVESDPRV
                     TTPAHLQTLARLGFTRLSLGIQDFDPRVQQAVNRVQTES"
BASE COUNT         1307 a         2594 c         2614 g         1327 t
ORIGIN      
        1 gatgccgacg gtgaaatggt tcttcggggc ggacttcttc agctcgtcga acaccgcctt
       61 ggccatcgcg ggcgtgaact ctttggaggc cagcccgtag cggccgccgc cgacgcgcgg
      121 cgcggcgacg aagggcgagg cgtcgtccat caggctctcg ccgacggccg tcagaacgtc
      181 ctggtagagc ggctcgcccg ccgcgcccgg ctccttcgtg cggtcgagaa cggcgacggc
      241 ccgcgctgtt cttggaatcg ccgacagaag gtcacgggcc gagaacgggc ggtaaagtct
      301 cactttgacg aggccgactc tttcgccgcg cgcaagcagc gcctcgaccg tctcttccgc
      361 cgcctcgcag cccgacccca tcaagacgac gacgcgctcg gcctgcgggt ggccgacgta
      421 atcgaacagg cggtactggc ggcccgtaag cgcggcgagc ttatccatcg tttcctgaac
      481 gatgctgggt gtggcgaggt agaaagggtt cgacgcctcg cgcgcctgaa agaacacgtc
      541 ggggttctgc gccgtgccgc gcataacggg gcggtcgggc gtaagtgccc gaccgcggtg
      601 cgcgcggacg agttccggat cgatgagcgc gcgcatgtcc tcgtccgtga gcggttcgat
      661 cttctgcact tcgtgcgagg tgcggaagcc gtcgaagacg tggaggaagg ggacgcgggc
      721 gcgcagggtc gcggcctgcg agacgagagc gaagtccatc acctcctgca cgctgttggc
      781 gaagagcatc gcccacccgc aggagcgtgt cgtcatcacg tccgagtgat cgccgaagat
      841 ggagagcgcg tgcgtggcga cggcacgggc ggcgatgtga aagaccgttg ccgtctgctc
      901 gcccgcgatt ttgaacatgt tcgggatcat cagcaggagg ccctgactgg cggtgaaggt
      961 cgtcgtcagg gagccggtct ggagcgtccc gtggacggcc cccgcggcgc cgccctccga
     1021 ctgcatctcg cgaactacgg gcaccacgcc ccagatgttc tggcggcctt cggcggccca
     1081 tgcgtcggcg ttctcgccca tgtttgacga gggcgtgatc gggtagatgg cgatgacctc
     1141 gttcgtcttg taggcgacgt gcgaggccgc gtcattgccg tccatcatgg tctgcttgcg
     1201 ctgcatcgtg tgtaatctca cccagaggcg gtctgtcttg tgcgaattct tccccatgcg
     1261 ggcgtctcaa ccgcggaaat gcttgccgca gcgcccgcgc catgaaggag tgcaaaccgt
     1321 gggccggaag gtgaagtgag cagtaagcag taagcagtga gcagtggaag acatttttta
     1381 ccgctcactg cttactgctt actgctcacc ttcttcgggt acactgcttg ccgtaagaca
     1441 atcattgcgc ccggctcgcg cgtgggaggg ttgatgaccg taaagcagaa ctggcaggca
     1501 cagtaccggg aaaaacttaa agacgcggcg cgcgccgtcc gcgcggtgcg ccgcgggcag
     1561 aaggtcttca tcgggtcggg ggcggccgag ccgcaggcgc tcgtgcgcgc gctctcagac
     1621 cgcgccgaag aactcgccga caccgagatt taccacatca tgacgctcgg catcgccccc
     1681 tatgccgagt cgaaattcgc gcgccacttc cgccacaacg ccttcttcat cggcaccaac
     1741 gtgcgcgcgg caatcgagga gggacgcgcc gactacacgc ccgtcttcct ctccgaagtc
     1801 cccaaactct tccgcacgcg ccgcattccg ctcgacgtgg cgctcatctc cgtcagcccg
     1861 cccgacgagc acggctactg ctcttacggc gtctcggtgg acgtggtcaa ggccgccgcc
     1921 gagtcggcca cgttggtcat cgccgaggtc aaccggcaga tgccgcgcac gctcggcgac
     1981 agcttcatcc acgtcagccg catcgactgc ctcgtcgagt cggacgcgcc cctgcccgtg
     2041 ctcgcgcaag gccggccgga cgacgtggcg atggagatcg ggcgccacgt cgccagcctc
     2101 atcgacgatg gctccaccat ccagatgggc atcggcgtca tccccaacgc cgtgctcgca
     2161 cagctcggag gcaagcgccg cctcggcgtc cacaccgaga tgttctccga cggcgtcatc
     2221 gacctcatcg agtcgggcgt catcgactgc tcgaagaaga cgcttctggc cggcaaggtc
     2281 gtctccagtt tctgcatggg caccccccgg ctctacgagt acgtagacaa caatccgctc
     2341 ttcgagttcc gctccgtgga gtttgtgaac gacccgttcc agatcgcgcg caacgagaag
     2401 atggtcgcca tcaacgccgc aatcgaggtg gatttgacgg ggcaggtcgc cgccgactcc
     2461 atcggcagcc gcttctacag cggcatcggc ggccaggtcg atttcatccg cggcgcggcc
     2521 cgctccgagg gcggcaagcc cgtcatcgcc ctgcgctcga ccgccaaggg cgacaccctc
     2581 tcgcgcatcg tcccgcgcct gcaaggggcg ggcgtcgtca ccacgcgcgg cgacgtgcac
     2641 tacatcgtca ccgagtacgg cgtggccgac ctctggggcc gaaacgtccg cgagcgcgcg
     2701 atggcgctaa tcaacatcgc gcacccgcgc ttccgacagg agttgctcgc cgaggccaag
     2761 cgggcgcgcc tcgtctacga cgaccaaatc ctgctccaca cagcctccta cccgaagcag
     2821 tgggagcgga ttgagccttc aaaggacggg acgagcgtct acctgcgcgc cgttaagccg
     2881 accgacgagg cgatgatgaa ggagctgttc tacacctgct cggagcagag cctctacgac
     2941 cgctactttc aggtcgtcaa ggcgatgccg cacgagaagt tgcagcgcat cgtcaacgtc
     3001 gattatgaga atgagatgtc ggtcgtggcg cggatgcgcg agggcgggca cgagcgcatc
     3061 gtcggggccg ccagctacac gctgaacccg gcgacgaaca cggccgacgt ggccttcctc
     3121 gtccacgacg agtaccaggc gcgagggcta ggcacgcttc tcctgcaaca cctgatgcgg
     3181 atcgcccgct cgaagggcgt ggccgggttt acggccgacg tgctggcgca caaccacgcg
     3241 atgctcaagg tcttccacaa ctcggggtgc gagatgaagt cgtcgctcgc cgacaacgtc
     3301 taccaactgg agttttattt cgaccgggcg caaaagggaa gcggcgcggg cgaacgcgac
     3361 gcggcggagg cggcggactc ctcagcggcg tccctctgaa gcgacgacgc gccggccgcg
     3421 tcgcgcgcgt cgggcgtgag gcagcacaca tggtcgagca cggacactcg ccggaggcga
     3481 tcagggaacg tctcgccgaa ggcccacgac acagctacct gcgcgactgg gtttacggcg
     3541 ggatagacgg ggccgtgacc accttcgccg tcgtctccgg cgtgatcggc gcgcagctcc
     3601 cgcccgccgt catcctcgtc gtcggcgcgg ccaacatcct ggccgacggg ttttcgatgg
     3661 ccgcctccaa ctacctcggc acgaagacgg agcttgagga gcgggagtac ctgaggcggg
     3721 tcgagcaccg gcacatcgaa gacttccctg agggcgagcg cgaggaggtg cgtcagattt
     3781 tcgctcagaa gggcttcgag ggggaggagc tggagcgcgt cgtcgagctg ataacttcag
     3841 accacgcgcg ttgggtgaac acgatgctga cggaggagta cggcctcgcc ccggaggtgc
     3901 gctcgcccgt gcgcgcggcc gcggcaacct tcagcgcctt catggtgtgc ggcgtcgcgc
     3961 cgctcctgcc cttcgtcctc gccgccccgc gtgccttcac cctctcggca gtcctgaccg
     4021 gcgcggcctt cttcgccgtc gggtcggcca agagcttctg gtcgccgacg ccgtggtggc
     4081 gctcgggcct gacgacgctc ctcatcggcg ccgtcgccgc gtgcctcgcc tacgtcgtcg
     4141 gcgtgctgac gagggggctg ctctagcgtc agtagccggc gtggcttggg ggcggagtct
     4201 tgccctccac gcgggtcgca acgccgccgt gcgggtcggc agcgtgctca cgcatcaggc
     4261 gacggatcag cgcgccatcg acgccccact gttcgagtat cacccgcacc ttctccctgt
     4321 cccccggcac atcgctgaag agcagttctc catgcgtcga atctctctct gccatgatcg
     4381 actccttgcg tggccgcgcg cgggcggcta acgcgtcttc tgcatggaca ccgtgacgag
     4441 cggcgcatca ccggccgcgc ggctgtaaga gacccagtag ccggcgcgct ggtatttcac
     4501 gagcttgtag acagtgccgt cggcccgcgg cgtcacgtcc gcgccgaaca gacttttggg
     4561 cgtcggcacg ccactgccgc cgccgagaaa ttggacggag acggccatca ccttgcgcgc
     4621 ggggtcgtag aagatttggg ctgactccgt gtccgagaag ttgtagaagt cctgctcgtc
     4681 gcccttgtcg gccggctcgc cgagccgacg gcgggcctcc tctgcgctca tccctaactt
     4741 cacgcccttg tactcgcgca aactcggctc atccgtggtg gacgtgttga tgcccacctg
     4801 ggccgtggcc gggccggact gcgagggcgt ctgcgcgtgg acgagaagtg cgccgaaggc
     4861 gaagatcgcg agggcgagca ggacgagttc ggcgagcatc ctgaggtaag tgcgatccgt
     4921 ggttgcaatg aacatcgtct gtgcctcctt ccgggaagcg tccgccgggg cgggcgcgcc
     4981 ctttgccacg tgtgcgtcgc aatccatgtg ccgacgccgc acgcgggtga aggacggcga
     5041 ttttatgggt tttcgtcggg cgcgcaggga gtaatgattc cgacccggcc ggctactggg
     5101 gatttctccg cccgccgcac gggatttttt ctcagtcgcg tttgttgccg ccccgcacgc
     5161 ggacttgaaa tgagcgcggc gcgggccgaa gatggtcacg atgcagatcg acaaggtcgc
     5221 cgcactcagg aacacgttgc tcttcggcga gctggatgag aagactttgc gcgcgctggc
     5281 ggggcgggcc gtcgagcgtc gctacgggcg cgacgaggtg cttttcctgg ccggcgaaga
     5341 ggcgcgcggc ctctactgca tcgtcgcggg ggcggtgcgc gcgttccggg aaagcctcga
     5401 cgggcgcgag caggtcatcc acgtcgagcg ctccggcgcg accgtggccg agcttccggt
     5461 cttcgacgac ggcccgtacc cctcgaccgt cgccgccgag gaggagacga ccgtgctctt
     5521 catcgacaag cgcgacgtgc gccgcctctc gctggagcac cccgagatcg cgctggccgc
     5581 gctcaaggtg ctcgccggaa gattgcgccg ctgcgccgaa ctcgtcgaaa ccctcagcct
     5641 gcgcgaggtc ggtcaacgcc tcgcgcgctt cctcctcgcg gaggcgcgca cgaaaggcgc
     5701 gccggcgggc gggggcttgc gcgtcgaact cacgcacagc aaccagcaga tcgccgcccg
     5761 cgtcgggtcg gtgcgcgaag tggtctcccg cgcgctcacg cgcctccagc acgacggcct
     5821 cgtccgactc gaaggccgcc gcctcacaat ccccgacgaa ggggcactcg cctccttcgc
     5881 gggcgaagaa tgaaaggtga gcggtaagcg gtaagcagtc agtaagccgc cggaggcggc
     5941 ggcagaatgc aacccacggc gcgcgtccgt ggggacagtc ggcagtgaaa gatgaagccc
     6001 cggaggggcg gaagaatctt caagcgaagc gacgcgcggc gtcgagattc ttccgcccct
     6061 ccggggctta gaaattttta tctgcctgtt accccggtct gacgaccggg gctacattct
     6121 gccgccgctt acagcggctc gttgctcact tgctgcccat gccctcttcc agtttcacgg
     6181 cgcgcgggaa gagcacgttg ttttcgaggt ggatgtgctg gtgcaagtcg cgctcaagct
     6241 cttcgagggc gtggtagagc gtctggtagc tgatgcaggc gtcggcgggc gcggcgtagt
     6301 cggcggtcgt cgcgcgcagc tcgcgcagga tgtcgcccgc cgtgtcgtgc tcgttcatca
     6361 tcacgcggat ggggttgccc acggtgccga agggcgcgta agggaggggg cggcccgcgg
     6421 cggcggcctg ctccatctgc ttgacgtagg ggaagaggat catctcctcc ttgaacatgt
     6481 gcgggcgcag gtcgtcgcag agttgttgga agagcgtgcg gacgcccagc agctctgggt
     6541 ggttctcgcc gtgcttcgag cagaccttct ccgtgagcgc ggtgatgcgc tccatctcgt
     6601 cgcgcgtgaa ggtgtggtgc ttgtcgagga tgtggtcgat cagctcggcg agcgtgcccg
     6661 acacaatctc ggccggcatt tcggccggcg cgctgcccgc ctgttcgatc aggcgcgtga
     6721 cctcttccac ggggacgccg gccacggcac aagcgtcgcc gagcgagcgg cccccgccgc
     6781 agcagtagtc aatcttcaac ttctcgaaga cgcgcgtggc gttgggcgtc tcgaccgcgt
     6841 actcgcgaac cgtcttttca gtgcataaag tcatttaagt agattctcct tcttatgcgc
     6901 cattctgagg ccggcgcccg cgccgcgcag tgacttaagt cacggatgaa aaaatttatc
     6961 gggagacccc aacgtcgggt cgtgagggac ggaagcgatt tcggccccga cacaggcacg
     7021 gaggttgcac ccgtcgtgcc acgagagctt ggcgggtggc ctcgtgcgga agtcacacag
     7081 gcgcgaggcc gaggtttacc ggcgaaattg aagtgatggg agacgcaccg cgcggcgcgg
     7141 acgaccggaa tggacgagcg gtggagggcg ccagtgggga ttattccaca gccccgacaa
     7201 gggaaaattc gcaggccggg gtgcggcccg aagtaacgtg acgatgagag atattttcat
     7261 gatcgctact gagaacgagc ttctgaaaaa gtacgacgtg cccggcccac gctacaccag
     7321 ctacccgacc atcctccact gggacaacac cccgacgccg gaagagtgga cgaactcggt
     7381 cgcccgcggg cttgacgagg cagaagcgct cggcgagggc gcggccgtct acgtccacat
     7441 ccccttctgc cgctcgctct gcacctactg cggctgcaac tcgcgcatca cccagagcaa
     7501 gacagtgggc gacccttacg tcgatgccgt gctccgcgag tgggcgctct atctcgacgc
     7561 actcaaacgc acaaactcgc tcccgctcgc cgaactgcat ctcggcggcg gcacgcccac
     7621 gtttctttcc ccggccgagc ttgagcgcct gatcggcggc atcctcggcg gcgtgagacg
     7681 cacggaggat tcggagttct ccgtagagtc tgacccgcgc gtgacgaccc ccgcacacct
     7741 ccagacgctc gcccgcctcg ggttcacgcg cctgagcctc ggcatacagg acttcgaccc
     7801 gcgcgtgcag caggccgtca accgcgtcca gaccgagagc ca
//
LOCUS       DASVES010000440         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1104600, whole genome shotgun sequence.
ACCESSION   DASVES010000440 DASVES010000000
VERSION     DASVES010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104600"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VF240_18075"
     CDS             complement(<1..657)
                     /locus_tag="VF240_18075"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HEX7177172.1"
                     /translation="MSINASRKIVLTALTFALALSVAPAALAQDNTSANARTVAAGQK
                     MKVKGIITRRDFDANTITVRDANGVDTGVQLSDTTQVKQKGGFFGGGKNYAVTNLLRG
                     LRVEVDGTGDSSGRLAARKVEFSGDDLRVAQSLEARVDPVESRVGTAEGRIGNVEQNA
                     QRMSGQLDELAALSNAARGGAKAAQETADAAVSGVRATNERISALDDYEAQQGVTLNF
                     R"
     gene            complement(1105..1443)
                     /locus_tag="VF240_18080"
     CDS             complement(1105..1443)
                     /locus_tag="VF240_18080"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX7177173.1"
                     /translation="MRYYITFAGISGTTTNRKYIGASRLLTYENDEAGSITFSKTFDK
                     ATQALMGAAFSDDTISWARAVCEEDGKVVFKTTFSNLRVSSFQQAGSDKSRIEYYSVD
                     YGGSTTEWPQ"
     gene            complement(1676..3196)
                     /locus_tag="VF240_18085"
     CDS             complement(1676..3196)
                     /locus_tag="VF240_18085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7177174.1"
                     /translation="MSTEVVMPQMGESIAEGTITRWLKKVGERVERDEPLFEISTDKV
                     DAEIPSPAAGTLIEVKFQEGETVEVNKVVAYIGEEGERAAAPPAQASAPAQGQEAQPA
                     AQQPSAPPAPAATQAAASETPPPPPASAPQTQPSAQEAQPARQQAAAAASASAQAPPS
                     PMGTGGASAAGAEARTASASPAGPSGDGGGERTVEELRRTRSSPLVRKIASEHDIDIS
                     RIEGTGASGRVTKNDILSFIESGGPVATEAPAAGAGSAPSMRDTAQAPSLPPPTQGDR
                     VEPMSVMRRKIAEHMVMSRRTSAHVTTVYEIDMTRIARLRERHKSSFKQATGTKLTFM
                     PFIFQAVTTAIRKFPVFNAQVSGDQIVYKRDINLGMAVALDWGLIVPVIKRADDLSLG
                     GLAKAANDLADRARAKQLKPDEVAGGTFTITNPGVFGGLFGTPIINQPQVAILGVGAI
                     EKRPKVITTDDGDDLIAIRHMAYFALSFDHRIIDGADAERFLAFVKETLEAGQFAV"
     gene            complement(3306..3581)
                     /locus_tag="VF240_18090"
     CDS             complement(3306..3581)
                     /locus_tag="VF240_18090"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX7177175.1"
                     /translation="MPKFLIEREIPGAGGLSGEELQAISQKSCGVLREMGPQIQWVES
                     YVTGDKVYCVYIAPDEASVREHAERGGFPANRVSEIKRMIDPTTAEG"
     gene            complement(3635..>3894)
                     /locus_tag="VF240_18095"
     CDS             complement(3635..>3894)
                     /locus_tag="VF240_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177176.1"
                     /translation="ARSGPAARGILSMSDQRAADDAFAFTVIDDDGEEVVIEVSEEDY
                     RREKTAGVEEESLLRPGRYRLKRGGFLARHPELKLKESRRA"
BASE COUNT          606 a         1269 c         1282 g          737 t
ORIGIN      
        1 gcggaagttc agggtcacgc cctgctgggc ctcgtagtcg tcaagggccg agatgcgctc
       61 gttggtggcg cgcacgcctg agacggcagc gtcggccgtc tcctgcgcgg ccttcgcgcc
      121 gccgcgcgcg gcgttggaga gtgcggccag ctcgtctaac tgacccgaca tccgctgcgc
      181 gttctgctcg acgttgccga tgcggccctc ggccgtgccg acgcgcgact ccaccggatc
      241 gactctggcc tcaagactct gcgccacccg caggtcgtct cccgagaact cgaccttgcg
      301 cgcggcgagg cggcccgaag agtcgcccgt gccatcgacc tcgacgcgaa ggccgcgcag
      361 gaggttcgtc acggcgtagt tcttgccgcc gccgaagaag ccgcccttct gtttgacctg
      421 cgtggtgtcg gagagctgca cgccggtgtc cacgccgttg gcgtcgcgga cggtgatggt
      481 gttggcgtca aagtcgcggc gcgtgatgat gcctttgacc ttcatcttct gcccggcggc
      541 gaccgtgcgc gcgttggccg aagtgttgtc ctgcgcgagc gcggcgggcg ccacagagag
      601 cgccagcgcg aacgtcaggg ccgtgagaac gatcttcctc gaagcattaa tactcatcgt
      661 cgtctaatct ccctctcgtg aaaaatgtcg ggcgtccttc acaccgccca cttcgcagac
      721 cgcggccggt gtcgggcgcg aggggccgaa atcggccgat ggttgcgctc tgccgcgcgg
      781 ggacttgcac gggcgctaac cgcccttcgc ggcttcgtgc cctccccgtc cgcgaccggc
      841 gcgaggcttt agtttgcgtg ttgcgtttac taacacacgg ttttaaacaa accgcatagt
      901 gcaagtcaag gggttggaca cagggcgggg gccaatacgt tgcgcgaaaa ccaatttatt
      961 acccaatcgt cacctcaccg caacattccg ccgcgtcgcg cctatcagcg gccagcctgt
     1021 gcgacgggaa acgtcgagac cgcaccgaag gaagctgctc atcacgttga acgcgccgcc
     1081 cccgcacaac agtaaagatt tgatctactg cggccattcc gtggtggagc ctccgtaatc
     1141 gacactgtag tactcgattc tagatttatc actgcccgcc tgctggaagc tgctgaccct
     1201 caggttgctg aaagtggtct tgaagacgac cttgccgtcc tcctcacaca ccgcccgcgc
     1261 ccagctgatg gtgtcgtcgg agaaggcggc gcccatgagg gcctgcgtgg ccttgtcgaa
     1321 ggtcttagaa aatgtgatag acccggcctc gtcgttctcg taggtgagga gccgggacgc
     1381 cccgatgtat ttccggttcg tggtcgtccc gctgatgcct gcgaaagtta tgtagtaacg
     1441 cacggctgga gcctcccgaa gtggtacgca catgggatca ccgaatgcga agggctacgg
     1501 cgaccaagtg gcgacaagag cgacgcgtct tatagcatga ggccggtgaa acacaaaaca
     1561 cttcacatcg aggccgaaag agaaatgggc gcgtgcggcg ggaaagaaga aagccgacgg
     1621 cgtcgaaact ccgtcggctt tcttgaacgt gtggcggcgg cggcgagcgt cgccgctaga
     1681 cggcgaactg tccggcttcg agcgtctcct tgacgaaggc gaggaagcgt tcggcgtcgg
     1741 cgccgtcgat gatgcggtgg tcgaaggaga gggcgaagta ggccatgtgg cggatggcga
     1801 tgaggtcgtc gccgtcgtcg gtggtgatga ccttcgggcg cttctcgatg gcgccgacgc
     1861 cgaggatggc cacctgcggc tggttgatga tgggcgtgcc gaagaggccg ccgaacactc
     1921 cggggttggt gatggtgaag gtgccgccgg ccacctcgtc gggcttgagc tgcttggcgc
     1981 gcgcgcgatc cgcaaggtcg ttggcggcct tggcgagtcc gccgagcgag aggtcgtcgg
     2041 cgcgcttgat gacggggacg atgaggcccc agtcgagcgc gacggccatg ccgaggttga
     2101 tgtcgcgctt gtagacgatc tggtcgccgg agacctgcgc gttgaagacg gggaactttc
     2161 gtatcgccgt cgtcaccgcc tgaaagatga acggcatgaa agtgagcttc gtccccgtcg
     2221 cctgtttgaa cgacgacttg tgacgctcgc gcagacgcgc gatgcgcgtc atgtcgatct
     2281 cgtagacggt cgtgacgtgc gccgacgtgc ggcgcgacat gaccatgtgc tcggcgatct
     2341 tgcggcgcat gacgctcatc ggctcgacgc ggtcgccctg cgtgggcggc ggaagcgacg
     2401 gggcttgcgc cgtgtcgcgc atcgacgggg ccgagcctgc gccggccgcc ggggcttcgg
     2461 tcgctacggg gccgcccgac tcgatgaagc tcaggatgtc gttcttcgtc acgcgcccgc
     2521 tcgcgcccgt gccctcgatg cgcgagatgt cgatgtcgtg ctcggaggcg atcttgcgca
     2581 caagcgggct ggagcgcgtg cgtcgcagct cctcgaccgt gcgctcgccg ccgccatctc
     2641 ccgaagggcc ggcgggtgac gccgaagcgg tgcgcgcctc ggcccctgct gcgcttgcgc
     2701 cgcccgtgcc catgggcgaa ggaggagcct gtgcggaggc ggacgccgcg gcggcggcct
     2761 gctgccgggc gggctgcgcc tcttgtgcgg acggttgcgt ctgcggcgcg gatgccggcg
     2821 gcggaggcgg agtctcggac gcggcggcct gcgtcgcggc gggtgcgggc ggcgcggatg
     2881 gttgctgtgc ggcgggctgt gcctcttgcc cctgagctgg tgcgctcgct tgtgccggcg
     2941 gcgcggcggc ccgttcgccc tcctcgccga tgtaggcgac gaccttgttg acctcgaccg
     3001 tctcgccctc ctggaacttg acctcgatca gagtccccgc ggccggcgac ggaatctcgg
     3061 cgtcaacctt gtcggtcgaa atctcgaaca gcggctcgtc gcgctccacg cgctcgccca
     3121 ccttcttcag ccaccgcgtg atcgtcccct ccgcgatgga ctcgcccatc tgcggcatga
     3181 caacttctgt gctcataagc tctccaaaaa aggagtcagg agtcaggagt caggagtcag
     3241 aataaaagcg gttgccgcct ttcttattct gactcctgac tcctgacttc cgacttcctg
     3301 tagttttatc cttccgccgt cgtcgggtcg atcatccgct tgatctccga gacgcggttg
     3361 gcggggaagc ccccgcgctc ggcgtgctcg cgcaccgacg cttcgtcggg cgcgatgtag
     3421 acgcagtaga ccttgtcacc cgtcacatag ctctcgaccc actgaatctg cggccccatc
     3481 tcgcgcaaca cgccgcacga cttctgtgag atggcctgca actcctcgcc cgacaaaccg
     3541 cccgcgccgg ggatttcgcg ctcgatcaga aacttcggca tcagtcttct cctttcgccg
     3601 ctcgcgcact gccgcgccgc tgcggcttta cctttcaagc tctccggctc tccttgagct
     3661 tcagctcggg gtggcgcgcg aggaagccgc cgcgtttcaa cctgtaccgg ccgggcctga
     3721 gcagcgactc ctcctcgact cccgccgtct tctcacgccg gtaatcctcc tctgacactt
     3781 cgatgaccac ctcttcgccg tcgtcatcaa tcacggtaaa ggcgaacgcg tcatcagccg
     3841 ctctttgatc gctcatactc agtatgccgc gagccgccgg gccgcttcga gcac
//
LOCUS       DASVES010000441        17894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_278260, whole genome shotgun sequence.
ACCESSION   DASVES010000441 DASVES010000000
VERSION     DASVES010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278260"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VF240_18100"
     CDS             <1..844
                     /locus_tag="VF240_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7177177.1"
                     /translation="QTREHILLARQVGVPAMVVFMNKVDAVDDAELLDLVELEVRELL
                     SAYEFPGDDIPVIRGSALGALNGEADWEKTVEELMASVDSFIPTPERDVEKPFLMPVE
                     DIFTIQGRGTVATGRVERGRVKVNETVEIIGIRPTRSTVCTGVEMFKKLLDEGVAGDN
                     VGLLLRGVERKDIERGQVIAKPGSITPHTVFKAEAYVLTKEEGGRHTPFFTGYRPQFY
                     FRTTDVTGVATLPEGVEMVMPGDNVAMEITLITPIAMEKGLRFAIREGGRTVGAGTIS
                     DIVE"
     gene            895..1215
                     /gene="rpsJ"
                     /locus_tag="VF240_18105"
     CDS             895..1215
                     /gene="rpsJ"
                     /locus_tag="VF240_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX7177178.1"
                     /translation="MLNEKIRIRLKAYDHRVLDQSTSEIVETAKRTGARVAGPIPLPT
                     SKNKWTVLRSPHVDKKSREQFEIRTHKRLMDILDPTPQTVDALMKLDLPAGVDVEIKA
                     FGRS"
     gene            1304..1951
                     /gene="rplC"
                     /locus_tag="VF240_18110"
     CDS             1304..1951
                     /gene="rplC"
                     /locus_tag="VF240_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX7177179.1"
                     /translation="MINGIIGKKVGMTQLFAADGTVTPVTVIKAGPCVVVQKKSAGGK
                     DGYDAVQLGLVEERPVKLKNVNKPMRGHFEKTGGGLPPTRVLKEFRTEGPVDQNVGDQ
                     VLVDLFGEGEHVNVIGRSKGRGFQGTIKRHNFSRGPESHGSMNVRKPGSIGQSAYPSR
                     VFKGMRSSGHMGDDRVTIKGLTVARVDVENNLLMIRGAVPGANGTVVVVTKAAKK"
     gene            2021..2725
                     /gene="rplD"
                     /locus_tag="VF240_18115"
     CDS             2021..2725
                     /gene="rplD"
                     /locus_tag="VF240_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX7177180.1"
                     /translation="MPTVKVRNLKNEEVGDLELSDAVFGAELNEGLIHSAVKSYMAGA
                     RQGTVGTKTRGDVSGSGRKLWKQKGTGRARIASIRSPLWKGGGNVHGPQPRDWAQPLP
                     KKMRRGALRSALSERLREGNLIVIDEFGFGQPKTKDFIAALSTLGAGGKTLIVDTLEN
                     DKLMLAARNVRRAKVVGASGVNVYDVLYHEKLLMTRTAIEELARALDPKSKTHRADAG
                     DGETAAADTTETEEAA"
     gene            2818..3123
                     /locus_tag="VF240_18120"
     CDS             2818..3123
                     /locus_tag="VF240_18120"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX7177181.1"
                     /translation="MRTLWDIIKSPVITEKALVLKEESQDARQLLTFRVDTKATKPEI
                     KSAVERIFNVQVDCVRTVNYMGKKVRRGRFEGRRPSWKKAYVTLKAGQDPFDYVESI"
     gene            3211..4041
                     /gene="rplB"
                     /locus_tag="VF240_18125"
     CDS             3211..4041
                     /gene="rplB"
                     /locus_tag="VF240_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX7177182.1"
                     /translation="MGIKKLTPTSPSRRYQTYLTRDDLTPGAEPEKSLLEPKKRISGR
                     NNDGHITVRRRGGGHKRHYRIIDFKRDKTGVPGRVTQIEYDPNRSAHIALITYLDGEK
                     RYILSPAGLEVGKSVMAGPEADILPGNALPIRNIPLGTQVHNIEMRPGKGGQMARSAG
                     AFAQLVAKEGDYAQLRMPSGEVRRVPVVCMATVGQVGNVEHENVSYGKAGRTRWHGIR
                     PHVRGVAMNPVDHPHGGGEGKTSGGRHPVTPWGQPTRGYKTRRNKRTTNMIVRDRRVK
                     "
     gene            4162..4458
                     /gene="rpsS"
                     /locus_tag="VF240_18130"
     CDS             4162..4458
                     /gene="rpsS"
                     /locus_tag="VF240_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX7177183.1"
                     /translation="MARSVKKGPFIDRHLEKKVEEIRSGSSRPPAIKTWSRRSTITPD
                     FVGLTFGVHNGKRHIPVYVTENMVGHKLGEFAPTRTFKGHSGTKAEKSSKPAGR"
     gene            4561..4935
                     /gene="rplV"
                     /locus_tag="VF240_18135"
                     /pseudo
     CDS             4561..4935
                     /gene="rplV"
                     /locus_tag="VF240_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            5161..5862
                     /gene="rpsC"
                     /locus_tag="VF240_18140"
                     /pseudo
     CDS             5161..5862
                     /gene="rpsC"
                     /locus_tag="VF240_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            6041..6469
                     /gene="rplP"
                     /locus_tag="VF240_18145"
     CDS             6041..6469
                     /gene="rplP"
                     /locus_tag="VF240_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX7177184.1"
                     /translation="MLMPKKVKHRKLMKGRMRGTATRGGAISFGEYGLKTMEPGWITD
                     RQIEAARIAMTRHVKRGGKIWIRMFPDKPITKKPAETRMGKGKGAPDHWVAVVKPGRI
                     LYEIEGVAEDIAREAMRLAAQKLPVKCKFVKRADQEGGAV"
     gene            6473..6700
                     /gene="rpmC"
                     /locus_tag="VF240_18150"
     CDS             6473..6700
                     /gene="rpmC"
                     /locus_tag="VF240_18150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX7177185.1"
                     /translation="MVKRREEIERLRGLSETELREEADRLKESLFRLNYKLALGEMDA
                     VKNMRREKRSLARIQTLMKERASEQSAAATK"
     gene            6713..7102
                     /gene="rpsQ"
                     /locus_tag="VF240_18155"
     CDS             6713..7102
                     /gene="rpsQ"
                     /locus_tag="VF240_18155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX7177186.1"
                     /translation="MAEETKEEVTTGAEAAEEVASGTSLAQAQAAGVDAQPGDATGAE
                     AGRRGRKAEKVGIVASDKMELTVVVRVDRLVKHPKYRRYVRRTSKFMAHDEKGAAIGD
                     KVRIVETRPLSARKRWRVVEIIQKAAK"
     gene            7200..7574
                     /gene="rplN"
                     /locus_tag="VF240_18160"
     CDS             7200..7574
                     /gene="rplN"
                     /locus_tag="VF240_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX7177187.1"
                     /translation="MAVQMQTSLEVADNSGARRVEMIQAVGGSTKATRASLGDKIKVT
                     VKEASPDGAVKKGTVHNAVVVRTRKEVRRKDGTYIRFDQNACVLIKPDDTPIGTRVFG
                     PVARELRERNFMKIVSLAPEVI"
     gene            7577..7918
                     /gene="rplX"
                     /locus_tag="VF240_18165"
     CDS             7577..7918
                     /gene="rplX"
                     /locus_tag="VF240_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX7177188.1"
                     /translation="MASKAKKRAKIKKGDQIVVITGRDKGKRGRVLEVRPVEGKVRVE
                     GIGVVKRHERPNPQKGTGGGIVEKEALVQISNVQLVDPQTGKGTRVRYLVNDDGSKTR
                     VAARGGNTLDK"
     gene            8020..8562
                     /gene="rplE"
                     /locus_tag="VF240_18170"
     CDS             8020..8562
                     /gene="rplE"
                     /locus_tag="VF240_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7177189.1"
                     /translation="MAARLRERYTKEVAPAIAKEFGITNPMAIPRLNKIVINMGLGEA
                     VANAKIVDTAADELRSVSGQKPVTTRAKKSIASFKLRQGMPIGSMVTLRGDRMYEFFD
                     RLVSIALPRVRDFRGVSPKAFDGRGNYTLGVRDQLIFPEIDFNKVDKTRGMNISIVTT
                     ARNDEQARALLRALGMPFRQ"
     gene            8650..8835
                     /locus_tag="VF240_18175"
     CDS             8650..8835
                     /locus_tag="VF240_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX7177190.1"
                     /translation="MAKKSLIAKSKRTPKFSARAYTRCQRCGRPRGYLRKFKLCRICF
                     RELALQGQIPGVIKSSW"
     gene            8968..9360
                     /gene="rpsH"
                     /locus_tag="VF240_18180"
     CDS             8968..9360
                     /gene="rpsH"
                     /locus_tag="VF240_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7177191.1"
                     /translation="MTDPIADMLTRMRNAIAARHSRVDIPASKLKLEIARILKEEGYV
                     NNFVVKGEGPRKVIRIFLRYDARGTSSISYLQRVSRPGRRVYLGAMEIPKVLGGYGIS
                     IVSTSRGLMSGKRARREKIGGEVLADIY"
     gene            9443..10003
                     /gene="rplF"
                     /locus_tag="VF240_18185"
     CDS             9443..10003
                     /gene="rplF"
                     /locus_tag="VF240_18185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7177192.1"
                     /translation="MSRIGKKVIAVPAGVTVTVQERELEVKGPKGTLRTPVPEGISFK
                     LEGDQLQAERRSDDYAAQHGLARALANNAVVGVTQGFTKQMDVVGVGYKADVQSKKIV
                     FSLGYSHPIEFPLPEGVEAKGERVPAKASIPQYQLTLTLSSIDKQMLGQVAAELHGLR
                     KPDPYKGKGVRYADVTLKLRPGKTGK"
     gene            10088..10438
                     /gene="rplR"
                     /locus_tag="VF240_18190"
                     /pseudo
     CDS             10088..10438
                     /gene="rplR"
                     /locus_tag="VF240_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            10511..11020
                     /gene="rpsE"
                     /locus_tag="VF240_18195"
     CDS             10511..11020
                     /gene="rpsE"
                     /locus_tag="VF240_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7177193.1"
                     /translation="MRQPKQRITAQGLGELSDQVISINRVTKVVKGGKNLSFAALVVV
                     GDHSGHVGFGTGKAREVPLAIKKAIEAAKKNLIRIPLINNTLPHPIVGTWGAGRVLIK
                     PASEGTGVIAGGAVRAVMQAAGIHDVRTKILGSTNPHNVVRATFDGLVNMKDPMEVAR
                     LRGKQVEDL"
     gene            11113..11322
                     /gene="rpmD"
                     /locus_tag="VF240_18200"
     CDS             11113..11322
                     /gene="rpmD"
                     /locus_tag="VF240_18200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX7177194.1"
                     /translation="MSDENREQAGGGGTVRIQYYRSAIGFPKTQKQIVRSVGLTKLNQ
                     TVERPDTASTRGVVQKVPHLLRIIE"
     gene            11409..11912
                     /gene="rplO"
                     /locus_tag="VF240_18205"
     CDS             11409..11912
                     /gene="rplO"
                     /locus_tag="VF240_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX7177195.1"
                     /translation="MAIGLNNLHPTEGSTHKKKRVGRGPGSGLGKTAGRGNKGQKSRS
                     GYSSKVGFEGGQMPLQRRLPKRGFTNIFKKQWTEITLAALDAAFADNDDVTPDVMHAR
                     GVIAKGKRDVVVLGTGEINKPLRVSAHRFTKSAREKIEGAGGTATLIESRKKKADESA
                     PDEGQEQ"
     gene            12163..13578
                     /gene="secY"
                     /locus_tag="VF240_18210"
     CDS             12163..13578
                     /gene="secY"
                     /locus_tag="VF240_18210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7177196.1"
                     /translation="MEKFFAAVRNMFSIPDLRKRLLFTLGLLAVYRLGAHVTTPGINQ
                     DQLKRVWESIGSTLLGVLDLFSGGNFQTISIFALGVTPYITASIILQLMTTVFPQLKK
                     LQEEGEAGRQKINQYTRYLTVVLCSFQTFIVARWLQANDVLPDGGWYVASTVLTLTTG
                     TVFVMWLGEQITERGVGNGISLLIFAGIVIGLPSGVGQVLERVRGGDTFATVGVLALV
                     VVLVALIAFVVFVESARRKVPISYAKRHVGRQLVGGQQTSMPLKINMGGVIPVIFASS
                     VLAIPQTVLQFLAYDPNVQDWRFSVHSFFQQFHGGDPYYELFFISLIVIFTFFYITII
                     FNVEEVADNLRKHGGFIPGIRPGRNTAEFLNTILTRLTTVGAIYLALVALIPQIMLSG
                     FRVGRLPFVGESLYAFFTSTPGLGWVPTGMGYQFFFGGTSLLILVGVAMDTVAQIEAQ
                     LVMRNYEGFFGPGSRLRGRRG"
     gene            13668..14324
                     /locus_tag="VF240_18215"
     CDS             13668..14324
                     /locus_tag="VF240_18215"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7177197.1"
                     /translation="MSKIVVIMGAPGAGKGTQARLVQERLGLPQISTGDIFRAMKQAQ
                     TPLAEEVRAIMAAGTLVPDEVTVRVVEERTARDDCRDGYILDGFPRTPAQAEMLERLA
                     ASQGHEIVAALVDVPFDILEKRVTGRRTCPVCGEIYNVHFKPPQSDETCDLHPDARLD
                     RRADDNAETIKARLATYERNTQPLIDYYERSGRLRRVDGTREPESIYADIERVVISDE
                     "
     gene            14394..15179
                     /gene="map"
                     /locus_tag="VF240_18220"
     CDS             14394..15179
                     /gene="map"
                     /locus_tag="VF240_18220"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7177198.1"
                     /translation="MIIGKSKKELEKMRAAGRLIAQVREELRRRVAPGVTTLELDLAA
                     ERMIRDAGALPTFKGYHGFPYSICASVNEQVVHGFPSTYALKDGDLFSLDCGATLEGF
                     VGDTATTIPVGQVSEDRLKLIRVAEECLERAIAECRPGKNLGDLGWAVQEHAEAHGYG
                     VVRDYVGHGIGRRMHEDPQIPNYGKPGKGAAIRAGYVFAIEPMLNMGTHQTKVLADGW
                     TVVTLDGQPSAHVEHTVAITEEGPEILTSVAEGVAREAAPVGA"
     gene            15427..15540
                     /gene="rpmJ"
                     /locus_tag="VF240_18225"
     CDS             15427..15540
                     /gene="rpmJ"
                     /locus_tag="VF240_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX7177199.1"
                     /translation="MKVRASVKKMCDNCKIIHRKGVVRVICTNPKHKQRQG"
     gene            15609..15992
                     /gene="rpsM"
                     /locus_tag="VF240_18230"
     CDS             15609..15992
                     /gene="rpsM"
                     /locus_tag="VF240_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX7177200.1"
                     /translation="MARVVGVDLPVNKRVQIGLTYIYGIGRARATSILREADVLPDTR
                     VRDLDEGQLNRIRSVIEGQGEVEGDLRKRVQMDIKRLMDIGCYRGLRHRRGLPTRGQR
                     THTNARTRKGPRRATVAKKKAPGKK"
     gene            16082..16489
                     /gene="rpsK"
                     /locus_tag="VF240_18235"
     CDS             16082..16489
                     /gene="rpsK"
                     /locus_tag="VF240_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7177201.1"
                     /translation="MAKAAAGGGKKKVFRKKEKKNIPTGIVHISASFNNTLISITDNE
                     GNLIAQSSSGARGFRGSRKGTPFAAQQAAYEAANKAKDAGMQQCEVHVKGPGGGRESA
                     IRAIHNAGIRVLAIRDTTPIPHNGCRPPKRRRV"
     gene            16672..17310
                     /gene="rpsD"
                     /locus_tag="VF240_18240"
     CDS             16672..17310
                     /gene="rpsD"
                     /locus_tag="VF240_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7177202.1"
                     /translation="MARYRGAVCRLCRREGAKLFLKGDRCYKPSCPIEKRGTQPPGQH
                     GNTLRRGKALQGYGQQLREKQKVKRIYFVLEGQFRNYFEKAARQKGITGENLLTLLER
                     RLDNTVYRAGFATSRRQARQLVNHGHIEVNGRKVDIPSFQVKAGDQIGVRETSRKNPH
                     IEGAWQTAAGRGRPSWIAPGEGDMAARVATLPVRNDIDRSINEQLIVELYSK"
     gene            17434..>17894
                     /locus_tag="VF240_18245"
     CDS             17434..>17894
                     /locus_tag="VF240_18245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013366.4"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7177203.1"
                     /translation="MAIDQHNLWTGFQMPRRLAVEGETLTERYGRFSAQPFERGFGTT
                     IGNSLRRALLSSIEGAAITAVKIEGVEHEFSSIRGVVEDATDVILNLKQIPFMLHGAE
                     AKTLTIRKDGAGEVTSADIEHDGEVDVLDPNVYIATVSEGGSLTVEMRLRRG"
BASE COUNT         3750 a         5421 c         5818 g         2905 t
ORIGIN      
        1 gcagaccaga gagcacatcc ttttggcccg gcaggtgggc gtgccggcga tggtcgtctt
       61 tatgaacaag gtcgacgccg tcgatgacgc cgaactcctc gacctggtgg agttggaggt
      121 cagagaactg ctttcggcct atgagttccc cggcgacgac atcccggtca tccgcggctc
      181 ggcgctgggg gccttgaacg gggaggcgga ctgggagaag acggtcgagg agttgatggc
      241 gtcggtggat tccttcatcc cgacgccgga gcgggacgtg gagaagcctt tcctgatgcc
      301 ggtcgaggac attttcacga ttcaggggcg tgggacggtg gcgaccggca gggtcgagcg
      361 cgggcgggtg aaggtcaacg agacggtgga gatcatcggg attcggccca cccgctcgac
      421 ggtgtgcacg ggcgtggaga tgttcaagaa gttgttggac gagggggtgg cgggcgacaa
      481 cgtcggactc ttactcaggg gcgtggagcg caaggacatc gagcgcgggc aggtgatcgc
      541 caagccgggc agcatcacgc cgcacacggt cttcaaggcc gaggcttacg tgctgacgaa
      601 ggaggagggc ggcagacaca cgccgttttt tacggggtat cggccgcagt tctacttccg
      661 gacgacggac gtgacggggg tggcgactct gcccgagggg gtggagatgg tgatgcctgg
      721 ggataacgtg gcgatggaga tcacgctgat cacgccgatt gcgatggaga aggggttgcg
      781 cttcgccatc cgcgagggcg gccgcacggt cggcgccggc accatctccg acatcgtcga
      841 gtagtagctc atagcttagt gctgaaagct gaaggctgaa agcttagtaa aatcatgctg
      901 aacgaaaaga tcagaatccg gctgaaagct tacgaccacc gcgtgctcga ccagagcact
      961 tcggagatcg tcgagacggc caaacgcacg ggcgcgcgcg tggcggggcc gataccgctc
     1021 ccgacctcga agaacaagtg gacggtgctg cgctcgccgc acgtcgataa gaagtcgcgc
     1081 gagcagttcg agattcgcac gcacaagcgc ctgatggaca tcctcgaccc gacgccgcag
     1141 accgtggacg cgctgatgaa gctcgaccta cccgccggcg tcgatgtcga gatcaaggca
     1201 ttcggcagga gctagcgaga gcagtgacaa gtgatgagtg ataagtgata agacaatcgc
     1261 ttttctcatc acttatcact catcacttat cacgaggtta gagatgatta acggaatcat
     1321 cggcaagaaa gtcgggatga cgcagttgtt cgccgccgac ggcaccgtca cgcccgtcac
     1381 cgtcatcaag gcggggccgt gcgtggttgt gcagaagaag tcggccgggg gcaaagacgg
     1441 ctacgacgcc gtgcagctcg gactggtcga agaacggccc gtcaagctga agaacgtcaa
     1501 caagccgatg cgcggccact tcgagaagac gggcggagga ctccccccca cgcgcgtgct
     1561 caaggagttt cgcaccgagg ggccggtcga tcagaacgtg ggcgatcagg ttctggttga
     1621 tctcttcggc gagggcgagc acgtgaacgt catcggtcgc tcgaaggggc gcggcttcca
     1681 gggcaccatc aagcgtcaca acttcagccg cggccccgag tcgcacggct cgatgaacgt
     1741 ccgcaagccc ggctccatcg gccagtcggc ctacccgtcg cgtgtcttca agggcatgcg
     1801 ctcgtcgggc cacatgggcg acgaccgcgt caccatcaag gggctgacgg tcgcgcgcgt
     1861 ggacgtggag aacaatctcc tgatgatccg cggcgccgtg cccggcgcca acggcacggt
     1921 cgtcgtagtg acgaaagcag caaagaagtg atgagtgatg agtgatgaga aagagcagac
     1981 ggttcttctt atcacttatc acttatcacc ggagttgaat atgcctaccg ttaaggtgcg
     2041 caacttgaag aacgaagagg tcggcgacct cgaactctcc gatgccgtgt tcggcgcgga
     2101 gttgaacgag gggctgatcc acagcgccgt gaagtcctac atggcgggcg cccggcaggg
     2161 caccgtcggg acgaagacgc gcggcgacgt gtcggggtcg gggcgcaagc tctggaagca
     2221 gaaggggacg gggcgggcgc gtattgcgtc gatccgctcg ccgctctgga agggcggcgg
     2281 caacgtccac gggccgcagc cgcgcgactg ggcgcagccc ctgccgaaga agatgcgccg
     2341 cggcgctctg cgctcggctc tttcggagcg tctgcgcgag ggcaacctga tcgttatcga
     2401 cgagttcggc ttcggccagc cgaagaccaa agacttcatc gccgccctct ccacgctcgg
     2461 cgccgggggc aagacgctca tcgtcgatac gctggagaac gataaactga tgctggcggc
     2521 gcgcaacgtc cgccgcgcga aggtcgtcgg ggcgagcggc gtcaacgtct acgacgtgct
     2581 ctaccacgag aagctgttga tgacgcgcac ggccatcgaa gagttggcgc gcgctctcga
     2641 cccgaagtcg aagactcatc gtgcggacgc cggagacggc gagacggcgg cggcggacac
     2701 gacggaaacg gaggaggcgg cgtaaaagcc gtaaatcgaa aatcgtaaat cgtgaataga
     2761 taagagattg tctttaacga tttacgattc acgattgacg atttacgagg tttcaagatg
     2821 cgtacactgt gggacatcat caaatcgccg gtcatcacgg agaaggcgct cgtgctcaag
     2881 gaggagtcgc aggacgcccg ccagctcctg accttccgcg tggacacgaa ggccacgaag
     2941 cccgagatca agtcggccgt cgagcgaatt ttcaacgtcc aggtcgattg cgtgcggacg
     3001 gtcaactaca tgggcaagaa agtgcggcgc ggccgtttcg aggggcgccg cccgtcgtgg
     3061 aagaaggcct acgtcacgct caaggcgggg caggacccgt tcgactacgt ggaaagcatt
     3121 taagagcaga ggtcagaggt tagaggctag aggttagtaa aaacttctgg cactgacctc
     3181 tgacctctag cctctaacct ctggattgtt atgggcatca agaagctaac accgacatcg
     3241 ccatcgcggc gctaccagac gtacctgacg agggatgatc tgacgccggg agcggagcct
     3301 gagaagtcgc tgctggagcc gaagaagcgg atttcggggc gcaacaacga cggccacatc
     3361 actgtgcgcc gccgcggcgg gggccacaag cgccactacc gcatcatcga tttcaagcgc
     3421 gacaagacgg gcgtgcccgg ccgcgtcacg cagatcgagt acgacccgaa ccgctcggcc
     3481 cacatcgcgc tcatcaccta tctcgacggc gagaagcgct acatcctgtc gcccgccggg
     3541 ctggaagtcg ggaagagtgt gatggccggc cccgaggccg acatcctgcc cggcaacgcc
     3601 ttgcccatcc gcaacatccc gctgggcacg caggtgcaca acatcgagat gcggcccggc
     3661 aagggcgggc agatggcgcg ctcggccggg gcgttcgcgc aactcgtcgc caaagagggc
     3721 gactacgcgc agcttcggat gccgtcgggc gaagtccgcc gcgtccccgt cgtctgcatg
     3781 gcgaccgtgg gacaggtcgg caacgtcgag cacgaaaacg tctcctacgg caaggccggg
     3841 cgcacgcgct ggcacggcat tcggccgcac gtccgcggcg tggcgatgaa cccggtggat
     3901 cacccgcacg gcggcggcga gggcaaaact agcggcggac gccaccccgt caccccgtgg
     3961 ggccagccca cccgcggcta caagacgcgc aggaacaagc gcacgaccaa catgatcgtg
     4021 cgcgacagaa gagttaaata agggatgaag gatgaggggt gaaaacgtgg tgatgaagat
     4081 tgagtggctc cgttaagcgg ctcttaattc atccctcatc cctccgccct cagccctcga
     4141 aagtggagcg aagcgaaacg catggcacgt tcagttaaaa aaggcccgtt catcgacagg
     4201 cacctggaga agaaggtcga ggagattcgg tcgggcagca gccggccgcc ggccatcaag
     4261 acttggtcgc gccgctcgac catcacgccc gacttcgtcg gcctgacgtt cggcgtccac
     4321 aacggcaagc gacacatccc cgtctacgtc acggagaaca tggtcggcca caagctcggc
     4381 gagttcgccc cgacgcgcac cttcaagggc cactcgggca cgaaggccga gaagtcgtcg
     4441 aagccggcgg gcagatagca agccgtgata agtgatgagt gataagtgat aagacaaaac
     4501 acaaccgtct ttctctttac tcatcactca tcactcatca cttatcactt aggttgagtc
     4561 atggaagcta aagcgagtgc gaagtatcta caggggtcgg cgcagaaggc gcggctcgtg
     4621 gtggacatga tccgcggcaa gaacgtgaat caggcgctgg ccattctcca gttcacgaac
     4681 aagcgcgcgg ccgacgccat cgagaagtgc ctgcgctcgg cgattgccaa cgcgaccgag
     4741 tcggccgaga aggccaacgt cgccgtcgat cccgacgacc tttgggtcaa ggcgtgctac
     4801 gtggacatgg ggccgaccaa gaagcgcttc cgcttccgcc ccgcgccgca gggccgcgcc
     4861 taccgcgagc gccgacactt ctgccacgtc accgtgcatg tgtcgagtga tggcaaggaa
     4921 gacaccctcg cgagcgcgaa gggcaagggc ggcgcgaagc gcgcggcgcg cgacgcgggc
     4981 ggctcgaaaa ccgcggcccg gcccgccgcc aaggcgaaga cgggcgctaa acgcgccgcc
     5041 gcgccgaagg ccgctgctca ggccgagaag aaagagactg gtaaggccgc ctcgaagacc
     5101 aagacgaccg ccgccaccac cgaatcaacg tctcaggaat agcgaacgga gaagacataa
     5161 gtgggtcaga aagttcatcc atacggcttc cgcctcggct acaacaagga ctggcattcg
     5221 cactggttcg ccaagaagca gtacggcgac tacctccttg aagacctccg gctcaagcgg
     5281 gaactcaagg gccgcttcgc cggcgcgggc gtctcgcaca tcgacgtgga gcgcgcggcc
     5341 aaccgcctga agatcatcat ctacacctcg cgccccggca tcatcatcgg gcgcaagggc
     5401 gcggagatcg acaagctcaa ggccgacatc gcctcgcgca ccggaagaga agtcctggtc
     5461 tcgattcagg aagtgcataa gccggaactc aacgcgcagc ttcaggccga gaagatcgcc
     5521 gcgcagctgg agaagcgcat cgccttccgc cgcgccatcc gcaaggccgt cgaagagtct
     5581 ctgcgcttcg gcgccgaggg catcaagacg aaggtctccg gtcgccttaa cggcgccgag
     5641 atcgcccgca gcgagtggca cttgcagggg cgtctgccct tgcacacgct gcgcgccgac
     5701 atcgactacg gcttcgccga ggcgcacacg acctacggca tcatcggcgt caagtgctgg
     5761 atttaccgcg gcgaaattct cgacgcgcga gcggcgatgg cgcgcggcac gcagaccgac
     5821 ccgatcaacg agccgcgcag ccagcgcggc gacggccgtc ggccccggcg cgaccgtgaa
     5881 gacggcgacc gtccgcgcgg cgaccgcggg cgcgggcgtg gcggcgacag gtcataagca
     5941 aggtggtaag tgataagtga taagtgataa gtaaagagaa aaacggattt tcttatcact
     6001 cattacttat cactcatcac ggcgaacgga gtgagcaatc atgttgatgc cgaagaaggt
     6061 caagcaccgg aagctgatga aggggcgcat gcgcgggacg gcaacgcgcg gcggcgccat
     6121 cagtttcggc gagtacggcc tgaagacgat ggagccgggg tggatcaccg accggcagat
     6181 cgaggcggcg cgtattgcga tgacgcgtca cgtcaagcgc ggcggcaaaa tctggattcg
     6241 gatgttcccc gacaagccga tcaccaagaa gccggccgag acgcgcatgg gcaagggcaa
     6301 gggcgctccc gatcactggg tggcggtcgt caagcccgga agaattctct acgagatcga
     6361 gggcgtggcc gaggacatcg cgcgcgaggc gatgcggctg gccgcgcaga agctccccgt
     6421 caagtgcaag tttgtgaagc gcgcggatca ggaagggggc gcggtttaag ttatggtgaa
     6481 gagacgtgag gaaattgaac gtctgcgcgg cctgagcgag acggaactgc gcgaggaggc
     6541 cgaccgcctc aaggagtcgc tcttccgcct caactacaag ctcgcgctcg gcgagatgga
     6601 cgcggtcaag aacatgcgcc gcgagaagag gtcgctggcg cgcatccaga cgttgatgaa
     6661 ggaacgcgcc agcgagcagt cggcggcggc gacgaagtaa agaggtttcg agatggcaga
     6721 agagacgaaa gaggaagtga cgaccggcgc ggaggccgct gaagaagtgg cttcgggcac
     6781 gagtctggcg caggcgcagg ccgcgggcgt ggacgcgcag ccgggcgacg ccacgggcgc
     6841 cgaggcggga cggcgcgggc gtaaggccga gaaggtcggc atcgtcgcct ccgacaagat
     6901 ggagctgacg gtggtggtgc gtgttgaccg tctggtcaag catccgaaat accgccgcta
     6961 cgtccgccgc acgtcgaagt ttatggcgca cgacgaaaag ggcgccgcca tcggcgacaa
     7021 ggtgcgtatc gtcgagacgc gcccgctctc ggcgcgcaaa cgctggcgcg tcgtcgagat
     7081 cattcagaag gcggcaaagt agcccgtgat aagtgatgag tgataagtga taagaaagag
     7141 aaaagcggat tttcttatca ctcatcactt atcactcatc actcgaacgg agtgagctta
     7201 tggcagtcca gatgcagacc tctcttgagg tcgccgacaa ttcgggggcg cggcgcgtcg
     7261 agatgattca ggccgtgggc ggctcgacga aggcgacgcg cgccagcctc ggcgacaaga
     7321 tcaaggtgac ggtcaaagag gcttcgccgg acggggcggt gaagaagggg acggtgcata
     7381 acgccgtcgt cgtccgcacg cgcaaggaag tgcgtcgcaa ggacggcacc tacatccgct
     7441 tcgaccagaa cgcctgcgtc ctcatcaagc cggacgacac gcccatcggc acgcgcgtct
     7501 tcggccccgt ggccagggaa ctgcgcgaac gtaactttat gaagatcgtt tcgctcgcgc
     7561 cggaggtgat ttgaaagtgg catcgaaggc taagaagcgc gccaagatca agaagggcga
     7621 tcagatcgtc gtcatcacgg gccgcgacaa gggcaagcgc gggcgcgtgc tcgaagttcg
     7681 ccccgttgag ggcaaggtga gggtcgaggg catcggcgtg gtcaaacggc acgagcgccc
     7741 gaacccgcag aagggaacgg ggggcggcat cgtcgagaag gaagccctcg tccagatttc
     7801 caacgtgcag ttggttgacc cgcagacggg caagggcacg cgcgtgcgtt acctcgtcaa
     7861 cgacgacggc tcgaagacgc gcgtcgccgc ccgcggcggg aacacgctgg acaagtaacc
     7921 gacggtttag cgcagtagta atcgcattcc cggaaacgcc gcgccggact ccagtccgac
     7981 ggcgagaccc ggggacgaaa tgtgaggggc aagaggacaa tggcagcaag gctcagagaa
     8041 cgctacacga aggaagtcgc gccggcgatt gccaaggagt tcggcatcac gaacccgatg
     8101 gccatcccgc gactcaacaa gatcgtcatc aacatgggtc tgggcgaggc cgtggccaac
     8161 gccaagatcg tggacacggc ggccgacgag ctgaggtcgg tgtcgggcca gaagcccgtg
     8221 acgacccgcg ccaaaaaatc catcgcctcc ttcaagctgc ggcagggcat gcccatcggc
     8281 tcgatggtca cgctgcgggg cgaccggatg tacgagttct tcgaccgcct ggtgtcgatt
     8341 gcgctgccgc gcgtccgcga cttccgcggg gtgagtccga aggctttcga cgggcgcggc
     8401 aactacacgc tcggcgtgcg cgaccagttg atcttccccg agatcgattt caataaggtg
     8461 gacaagacgc gcggcatgaa catctccatc gtcacgaccg cgcgcaacga cgagcaggcg
     8521 agggcgcttc tgcgcgcgct cggcatgcct tttagacaat aagaggtcgg aggttcggag
     8581 gtcagaggtc agttaacaac tgtcttctac taacctctaa cctctaacct ctaacctctg
     8641 aggttttaga tggcgaagaa atcactgatt gccaagtcga aacggacgcc gaagttctcg
     8701 gctcgcgcct acacccgctg ccagcggtgc gggcggcccc gcggctacct ccgcaagttc
     8761 aaactatgtc gcatctgctt ccgcgaactg gcgttgcaag ggcagattcc cggcgtcatc
     8821 aagtcgagtt ggtgagggag cttttagctg ttagctttca gctattagct ttagttcgag
     8881 gctgtagttg aaagattcga gcggcgtggc gcggcttggc taacagctaa tggctaacag
     8941 ctaatagctt atttggagtt ttgagtaatg accgatccta ttgccgatat gctgacgcgg
     9001 atgcgcaacg cgattgcggc gaggcactcg cgcgttgaca tcccggcgtc gaagctaaaa
     9061 ctggagatcg cccgcatcct caaggaggag ggctacgtca acaacttcgt cgtcaagggc
     9121 gaaggcccgc gtaaagtcat tcgcatcttc ctgcgctacg acgcccgcgg cacctcgtcg
     9181 atctcgtacc tccagcgcgt ctcgcgcccc ggaaggcgcg tctacctcgg cgcgatggag
     9241 attccgaagg tgctgggcgg ctacggtatc agcatcgtct cgacctcgcg cggcctgatg
     9301 tcgggcaagc gcgcgcgccg cgagaagatc ggcggcgaag tgctggcgga tatttactag
     9361 gtgataagtt gtgagtgata agtgataaga aaatccgtct tatctcatca ctcatcactc
     9421 atcacttatc actgaggttc atatgtctcg tataggaaag aaagtcatcg cggtgccggc
     9481 gggcgtgacg gtcacggtgc aggagcgcga gcttgaggtg aaggggccga aggggacgct
     9541 gcggacgccc gtccccgagg gcatctcgtt caagctcgaa ggcgaccagc tccaggccga
     9601 gcgccgttcg gacgactacg ccgcccagca cgggctggcc cgggcgctgg cgaacaacgc
     9661 cgtcgtcggc gtcacgcagg gattcaccaa gcagatggac gtggtcggcg tcggctacaa
     9721 ggccgacgtg cagagcaaga agatcgtctt ctcgctcggc tactcgcacc ccatcgagtt
     9781 cccgctgccc gagggcgtcg aggcgaaagg cgagcgcgtc ccggcgaagg cgtcgattcc
     9841 gcagtatcag ttgacgctca cgctctccag catcgacaag cagatgctcg gacaggtcgc
     9901 cgccgagctg cacggcttgc gcaagcccga cccctacaag ggtaagggcg tgcgctacgc
     9961 cgacgtgacg ctcaagctgc gcccgggcaa gacgggcaaa tagcagtgat aagtgataag
    10021 tgatgagtga taagagaaaa cttcttactc atcacttatc actcatcact tatcactgag
    10081 gttagaaatg gcacagaagg atagagcgac tgttaggcgc gcggtgcaca ggcgcatccg
    10141 caagaaggtg ttggggtcgg gcgggcgccc gcgcctcgcc atctaccgca gcctgaacca
    10201 catctacgcc cagatcatcg acgacgagcg cggccagacc atcgcgtccg cctccaccac
    10261 ggagaaggac ttgcggggcg gcacgggcgg caacatcgag gccgcgcaac gcgtcggccg
    10321 ggcgattgcc gagcgcgccg tcgcgcaggg cgtctccagc gtcgtcttcg accgtggcgg
    10381 ctaccgttac cacgggcgcg tcaaggcgct ggccgacgcc gcccgcgaag ctggtctgaa
    10441 caaggaagag gcgccgcccg ccgcagccga gactgcggaa gaagcggccg tgactgccga
    10501 aggataatcg atgcgacaac cgaaacagag aatcacagcg caaggcttgg gtgagttgag
    10561 cgatcaggtc atctcgatca atcgcgtgac gaaggtcgtc aagggcggca agaacctgag
    10621 tttcgcggcg ctcgtcgtcg tcggcgacca ctcgggccac gtcgggttcg gcacgggcaa
    10681 ggcgcgcgaa gttcctctcg ccatcaagaa ggccatcgag gcggcgaaga agaacctgat
    10741 ccgcatccca ctcatcaaca acacgctgcc gcaccccatc gtcggcacgt ggggcgcggg
    10801 gcgcgtgctc atcaagccgg cttcggaagg gacgggcgtg atcgcgggcg gggccgtgcg
    10861 cgccgtgatg caggccgccg gcatccacga cgtgcgcacg aaaatactcg gctccaccaa
    10921 cccgcacaac gtcgtgcgcg cgaccttcga cggtctggtc aacatgaagg acccgatgga
    10981 agttgcgcgc ctgcgcggca agcaggtgga agatttgtaa aagccgttaa tcgtcaatcg
    11041 tcaatcgtga atagataaaa cctgttcccg acgattgacg attcacgatt aacgattgac
    11101 gaggtagcag taatgtctga cgagaataga gagcaggcgg gcggtggggg cacggttcgc
    11161 atccagtatt accggagcgc catcggcttc ccgaagacgc agaagcagat cgtccgcagt
    11221 gtcgggctga cgaagctcaa ccagacggtc gagcgccccg acacggcttc gacgcgcggc
    11281 gtcgtccaga aggtgccgca cctgttgaga atcatcgaat aagagttttt agtagtcagt
    11341 agtcagttat cagtaaagac taaactgaaa actgactact gaaaactgta tcgcgaacgg
    11401 agtgagcaat ggcaatcggt ctcaataacc tgcacccgac agagggttcg acgcacaaga
    11461 agaagcgcgt cgggcgcggc cccggctcgg ggctgggcaa gacggcgggg cgcggcaaca
    11521 agggtcagaa gtcgcgctcg ggctattcga gcaaggtcgg cttcgagggc gggcagatgc
    11581 cgctccagcg ccgcctgccc aaacgcggct tcaccaacat cttcaagaag cagtggacgg
    11641 agatcacgct cgccgcgctc gacgcggcct tcgccgataa tgatgacgtg acgcccgacg
    11701 tgatgcacgc gcgcggcgtc atcgccaagg gcaagcgcga cgtggtggtg ctcggcacgg
    11761 gcgagattaa caaacccctg cgcgtctcgg cccaccgctt caccaagagc gcccgcgaga
    11821 agatcgaggg agccggcggc acggccacgc tcatcgagtc gcggaagaag aaggccgacg
    11881 agtcggcgcc cgacgagggg caggagcagt agttgttgta gcagccaagc ccgttgaaaa
    11941 ttcggcccgg ccgttcacgc tgagggcgcc cgcggggagt tatcgcaacc cctcacgcgc
    12001 cttcagcgtt atttgctaaa aagctgttag cgattagctg ttagccttta gcttttccgt
    12061 ctctttcttc gctctcgttt cggcgaagat tgactaacga ataaagttca aagactaaca
    12121 gctaacagct aacagctaac agctaatcgc tgaaggctaa tcatggaaaa gtttttcgct
    12181 gccgtccgca acatgttcag catccccgac ctgcgtaagc gcctgctgtt tacgctgggg
    12241 ctgctcgcgg tctaccgtct cggcgcgcac gtcacgacgc cgggcatcaa ccaggatcag
    12301 ttgaagcgcg tctgggagag catcgggagc acgctcctcg gcgtcctcga cctcttctcc
    12361 ggcggcaact tccagaccat ctcgatcttc gccttgggcg tgacgcccta catcacggcc
    12421 tcgatcatcc tccagttgat gacgaccgtc ttcccgcagc tcaagaagct acaggaggag
    12481 ggcgaggcgg gccgccagaa gatcaaccag tacacgcgct acctgaccgt ggtgctctgc
    12541 tcgttccaga ccttcatcgt cgcgcgctgg ctccaggcca acgacgtgct gcccgacggc
    12601 ggctggtacg tcgcctcgac cgtgctcacg ctcaccacgg ggacggtctt cgtcatgtgg
    12661 ctcggcgagc agattacgga gcgcggcgtc ggcaacggca tctccctgct cattttcgcc
    12721 ggcatcgtca tcggcctgcc cagcggcgtc gggcaggtgc tcgagcgcgt ccgcggcggc
    12781 gacaccttcg ccaccgtcgg cgtcctcgcc ttagtcgttg tcctcgttgc gctcatcgcc
    12841 ttcgtcgtct tcgtcgagtc ggcccggcgc aaggtgccca tcagctatgc caagcggcac
    12901 gtcgggcggc agctcgtcgg cgggcagcag acgagcatgc cgctgaagat caacatgggc
    12961 ggcgtcatcc ccgtgatctt cgcctcgtcg gtgctggcca tcccgcagac cgtcctccag
    13021 ttcctcgcct acgacccgaa cgtgcaggac tggcgcttca gcgtccacag cttcttccag
    13081 cagttccacg gcggcgaccc ctactacgaa ctgttcttca tctcgctcat tgtcatcttc
    13141 accttcttct acatcacgat cattttcaac gtggaggagg tggccgacaa cctgcgtaag
    13201 cacggcggct tcatccccgg catccgcccc ggtaggaaca cggccgagtt tttgaacacg
    13261 attttgacgc gactgaccac ggtcggcgcc atctacctcg cgctcgtcgc gctgatcccg
    13321 cagatcatgc tgagcggctt tcgggtcggg cgcttgccct tcgtcggcga gtcgctctac
    13381 gccttcttca cctcgacgcc gggactcggg tgggtgccca ccggaatggg ctaccagttc
    13441 ttcttcggcg gcacttcctt gctcatcctc gtcggcgtgg cgatggacac cgtcgcgcag
    13501 atcgaggcgc agctcgtcat gcgtaactac gagggcttct tcggaccggg cagccgcctg
    13561 cgcgggcgca ggggataaag acagagaggt cggaggtcag aggttagagg tcagttatca
    13621 aaactgtttt cactaacctc taacctctaa cctctgacct ctgattcatg tccaagatcg
    13681 ttgtcatcat gggcgcgccg ggcgcgggga aggggacgca ggcgcgtctc gtccaggagc
    13741 gtttggggtt gccgcagatt tcgacgggcg acatcttccg cgccatgaag caggcgcaga
    13801 ctccactggc cgaggaggtg cgcgccatca tggccgccgg cacgctcgtc cccgatgagg
    13861 tgacggtgcg cgtcgtcgag gagcgcaccg cgcgcgacga ctgccgcgac ggctacatcc
    13921 tcgacggctt cccgcgcacg ccagcgcagg ccgagatgct cgaacggctc gccgcctcgc
    13981 aggggcacga gatcgtcgcc gccctcgtgg acgtgccctt cgacattctg gagaagcgcg
    14041 tcacgggccg gcgcacgtgc cccgtctgcg gcgagattta caatgtccac ttcaagccgc
    14101 cgcagtcgga cgagacctgc gatctccacc ccgacgcgcg gctcgaccgg cgcgccgacg
    14161 acaacgccga gacgatcaag gcgcgtctcg cgacctacga gcgcaacacg cagccgctca
    14221 tcgactacta tgagcgttcc ggccgcctcc gccgcgtaga cggcacgcgc gagccggagt
    14281 cgatttacgc ggacatcgaa agggtagtga taagtgatga gtgataagtg ataagaggaa
    14341 aacagtctac tctttcttat cacttatcac tcatcactta tcacggcttt taaatgatta
    14401 tcgggaagtc gaagaaggag ctggagaaga tgagggccgc cgggcgtctc atcgcgcagg
    14461 tgcgcgagga gttgcgccgg agggtcgcgc ccggcgtcac cacgctcgaa ctcgacctcg
    14521 ccgccgagcg gatgattcgc gacgccgggg cgctgcccac cttcaagggc taccacggct
    14581 ttccctattc catctgcgcc tccgtcaacg agcaggtcgt ccacggcttc ccgtccacct
    14641 acgcgctgaa ggacggcgac ctcttctcgc tcgactgcgg ggcgacgctc gaaggcttcg
    14701 tcggcgacac ggcgacgacg attcccgtcg ggcaggtaag cgaagaccgc ttgaagctaa
    14761 tccgcgtggc ggaagagtgt ctggagcgcg ccatcgccga gtgccggccg gggaagaatc
    14821 tgggcgacct gggctgggcc gtgcaggagc acgccgaggc gcatggctac ggggtcgtgc
    14881 gcgactacgt cggccacggc atcggccgga ggatgcacga agacccgcag ataccgaact
    14941 acggcaagcc gggcaagggc gcggcgattc gcgccggcta cgtcttcgcc atcgagccga
    15001 tgctcaacat gggcacgcac cagacgaaag tgctcgccga tggctggacg gtcgtcacgc
    15061 tcgacgggca gccgagcgcc cacgtcgagc acacggtcgc catcacggag gaagggccgg
    15121 agatactgac gagcgtcgcc gaaggcgtgg cgcgggaggc ggcccccgtc ggggcctgag
    15181 cggtgcggaa acggttgtga gcgatgccct tagcggcttg gacgctcggc cgtagctatg
    15241 ttattatcca caatttgcca gccgcgttaa atttttacct gacgatgcag tggggtatgg
    15301 caaaggatga agcaaccggg gtgacggcta atgagtttgc gtcctacgac ctcatccgcg
    15361 cgccgggtca cgtatcagta accagttagg cggcgcggct aagaattccg aagaaggtga
    15421 acggcgatga aagtaagggc ttcggtcaag aagatgtgcg acaactgcaa gatcatccac
    15481 cgcaagggcg tggtcagggt gatctgcacc aaccccaagc acaagcagcg gcaggggtag
    15541 gacgtgggaa tctcaggtgt gagatttcag atttgaaatt ttttagcgag actcggagat
    15601 aaaaggaaat ggctcgtgta gtcggcgtcg atcttccggt gaacaagcgg gtgcagatcg
    15661 gccttacata catctacggc atcgggcgcg cgcgcgccac ttccatcctg cgcgaggccg
    15721 acgtgctccc ggacacgcgc gtgcgcgacc tcgacgaggg gcagctcaac cgcatccgct
    15781 cggtcatcga gggacagggc gaggtggaag gcgacctccg caagcgcgtc cagatggaca
    15841 tcaagcggct gatggacatc ggctgctacc ggggcctcag gcaccgccgc ggtctcccca
    15901 cgcgcggcca gcgaacacac accaacgcgc gcacccgcaa ggggccgcgc cgcgccacgg
    15961 tcgccaagaa gaaggcgccg ggcaagaagt aaaaagcaga ggtcggaggt tagaggttag
    16021 aggtcagtta caaactgcct tcactaacct ctaacctcta acctctaacc tctgaggttt
    16081 catggcaaaa gcagcagcag gcggtggcaa gaagaaggtc tttcggaaga aagagaagaa
    16141 gaacatcccg acggggatcg ttcacatctc ggcgtcgttc aacaacacgc tgatctcgat
    16201 cacggacaac gagggcaacc tcatcgcgca gtcctcctcg ggcgcccgcg gcttccgcgg
    16261 ctcgcgtaag gggacgccgt tcgccgcgca gcaggcggcc tacgaggcgg cgaacaaggc
    16321 caaggacgcg gggatgcagc agtgcgaggt gcacgttaag gggccgggcg gcggacgtga
    16381 gtcggccatc cgcgccatcc acaacgccgg catccgcgtg ctcgccatcc gcgacacgac
    16441 gccgatcccg cacaacggct gccgcccgcc gaagcggcgc agagtttaaa gagccggtga
    16501 taagtgatga gtgatgagtg ataagagaag aggcgctaag ctttttctca acactcatca
    16561 cttatcactc atcactttct catttaccta aaggatgaag ggtcgcgcgc gcgcgtggcg
    16621 tgcggtctgt aaacttttcc gtcttaagtt ttagaccgga ggcaaaatca agtggctagg
    16681 tatcgtggag cggtgtgccg tctgtgccgc cgcgaaggcg caaaactttt cctcaagggc
    16741 gaccgctgct acaagccgtc gtgccccatt gagaagcgcg gcacgcagcc gccgggtcag
    16801 cacggcaaca ccctgcggcg cggcaaggcg ctgcaaggct acgggcagca gttgcgcgaa
    16861 aagcaaaagg tcaagaggat ctacttcgtc ctcgaagggc agttccgcaa ctacttcgag
    16921 aaggccgccc ggcagaaggg catcacgggc gagaacctgc tcacgcttct ggagcgccgt
    16981 ctcgacaaca ccgtctaccg cgccgggttc gccacctcgc gccggcaggc tcgccagctc
    17041 gttaaccacg ggcacatcga ggtcaacggg cgcaaggtcg atatcccttc gtttcaggtc
    17101 aaggcgggcg accagatcgg cgtccgcgaa acgagccgca agaacccgca catcgagggc
    17161 gcgtggcaga cggccgccgg gcggggccgc ccctcctgga tcgctccggg cgaaggcgac
    17221 atggccgcgc gtgtggccac gcttcccgtg cgtaacgaca tcgaccgctc gatcaacgag
    17281 caactcatcg tcgagcttta cagcaagtag tctcggcgcg ccgctgtgtg cgcgcccccg
    17341 aagtcgccgc gccggagcgt gcggcggggg cgcgccggcg cgcactcttg gcgtcagcgc
    17401 tttccatagt caccggagta ttttataaga gccatggcca ttgatcagca caacctctgg
    17461 acaggatttc agatgccgcg ccgcctcgcc gtcgagggtg agacgctcac ggagcgctac
    17521 gggcgcttct ccgcccagcc cttcgagcgc ggcttcggca ccaccatcgg caactctctg
    17581 cgccgcgcgc tgctctcgtc gattgagggc gcggccatca ccgccgtcaa gatcgagggc
    17641 gtcgagcacg agttctcgtc gatccgcggc gtggtcgagg atgcgaccga cgtcatcctc
    17701 aacctcaagc agattccctt catgctccac ggcgccgagg ccaagaccct gaccatccgc
    17761 aaggacggcg cgggtgaggt cacttcggcc gacatcgagc acgacggcga agtcgatgtg
    17821 ctcgacccca acgtctacat cgccacggtc agcgagggcg gctcgctcac cgtcgagatg
    17881 cgcctgcggc gcgg
//
LOCUS       DASVES010000442        12759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1039929, whole genome shotgun sequence.
ACCESSION   DASVES010000442 DASVES010000000
VERSION     DASVES010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039929"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF240_18250"
     CDS             complement(<1..218)
                     /locus_tag="VF240_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177204.1"
                     /translation="MRFFKLLPALLLSLLCVVLLGAAPSRVSAQTQQAPLTNEEFLRL
                     VRQIPKDPDARMQIIEALRRRGIAFPLT"
     gene            complement(274..1416)
                     /gene="argE"
                     /locus_tag="VF240_18255"
     CDS             complement(274..1416)
                     /gene="argE"
                     /locus_tag="VF240_18255"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEX7177205.1"
                     /translation="MNVEETLAGLVRLDTRSSRTNVEFISHVAPLVEAAGLCARLLPY
                     TDEQGVKKIQMVAVAPREVRDDDEIGLALVGHTDTVPFDPAWTDALTLTERDGKLYGR
                     GACDTKGFIAAALTAIQQTDLARLRRPLALVLTADEEIGCLGAKRLSDARALRPRAAI
                     VGEPTGLQPVRAGKGYCLAEVTLRGREAHSAYPHLGASAVMRAARLITRIERISEDLK
                     GRAHEAFDPPHTTVNVGIIHGGAAKNIIPGECRFTLEWRPIPTETPEYFLELLRREID
                     ELRSTDADFDCALKVIRLDRGLETSADAPLVRALEEATGRRAGAVSFGTEAPQMAELG
                     AAAVVFGPGDIRVAHRTNEFVPRSELSECVSILARMIERFCVAEHQ"
     gene            complement(1568..3139)
                     /locus_tag="VF240_18260"
     CDS             complement(1568..3139)
                     /locus_tag="VF240_18260"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX7177206.1"
                     /translation="MSTSNQNTEPGAHITWVPDLIYADGRFRRDVGLTCDARGRVVAL
                     SPMSLLRTLTGGEPREGETHDITISDRYLATRFVRLRNRALLPGMVNAHSHAFQRVIR
                     GRTEYRSPAASDSFWTWREMMYRAAARLSPEDIYDASRMAFMEMMLTGVTSVGEFHYL
                     HRAPEGAAYDEANLLAKEVVRAARDVGLRIALLRVAYARSGYATPPNERQRRFIESDP
                     AEFIRNAEALDDDLARAEASNGDGAEAPRRAWAGVAPHSVRAVPVEYARAVAEFARGR
                     ALCVHMHVAEQPAEVAACVEETGFTPVALLADSGILDSRFTGVHAVHVTPEETEALAA
                     AGATVCACPTTERNLGDGVVPADEMFARGVRVALGTDSHAQIDLLEDARELEYHLRLQ
                     KLRRNVLAPPAAAGGADGEDRETHDEASQLPALAARLFDCATVAGAHSIGAGAGQLAP
                     GRAADFFTIDLEDPSVAGAAEDDLLPAIVFSMARTALRETAVGGRLVVSEGRHPAQEE
                     VVARFSSLQRKLWGR"
     gene            complement(3338..5182)
                     /locus_tag="VF240_18265"
     CDS             complement(3338..5182)
                     /locus_tag="VF240_18265"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX7177207.1"
                     /translation="MPPSRKCSLLFLIVAAVVLQAMPTKAQRTNPRPRAGAAQGQAGA
                     GRQLHAIFDAEWQWTMQEYPTFASSLGDLRYNDRWTDISVEAYERRHRHRQDVLARLS
                     RLERARMSPADQLNYDLFKKDLELAIEEHKFRAYLLPINQREGVQNSDELSDQLRFQT
                     VKDYDDWLARLRALPNYMDQSIALLREGKAAGILWPKVTMQRVPAQLDKQIVARAEDS
                     PFYKPFKNFHADIPAAERERLTRAASETISTGVVPAYRRLKEYFVAEYLPASYDKVGI
                     WQWRDGGAAYAYYARSYTTTGMTPEQIHERGLSEVARIRAEMQKIMTQVGFKGTHKEF
                     FKKLRTDDQFYYKKPEELLDAYRAISKKIDPHLVKVFKTIPRVPYGVIPIPDKIAPDT
                     TTAYYNQPAADGSRPGYYYVNLYKPESRPKWEMMALSLHEAVPGHHFQISRAMELGEI
                     PEFRRYGGYTAFVEGWGLYAESLGEDMGLYDDPYSKFGQLTYEMWRAVRLVVDTGMHH
                     MKWDRQRAIDYFLDNAAKTEQDIVNEIDRYITNPGQALAYKIGELKFKELRARAKSQL
                     GDKFDVREFHDVVLGSGAVPLDVLERIVDDWIRSQKKRAAEGQSARRR"
     gene            6020..8848
                     /locus_tag="VF240_18270"
     CDS             6020..8848
                     /locus_tag="VF240_18270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX7177208.1"
                     /translation="MLHLLRSVKFRARKSAGFALAWLLILSALPVAPGAAARTPKPGA
                     GAQDPAPAQQIPVIAPGSAYRQANLVSDVPGLAFVLDPLLVNPWGISLTATSPFWVAN
                     NGTSTTQLIRGDVGGAPVVLNPSPQTITIPGGLPTGTVANGVATEFVLPGACASAPCG
                     ANFLFASITGNITGWDPNAPAAGSTTAVIAASHPGHVYTGLAIANNGSGNFLYAADFA
                     NGRIDVFNSSFALQPAASFPFADPTIPTTMGNTYHPFNIQAIGSSLYVAYAKVGPGGL
                     DVEGVGNGFVRRFNANGVRDLTFGINNGALNSPWGITQAPATFGIFGGALLVGNFGRG
                     NPSIHAYNPTTGAFLGTLQDESGNGIVIDELWDLKFGNGGAGGDPNTLYFAAGIGHEE
                     HGLFGSLKPTTASATSLIQFSTDDYAISENSGHIDITVTRNGDVSGSATINYNTFDES
                     QPGHASQKSDYEIALGTLHFAPGETSKTFRILIVDDKDFAPFPGGNEGNEVVSLALSN
                     PTGAGVGLGSPNVVELTIIDDDALLPTTNPIDDATFFVRQHYLDFLNREPDPAGLAFW
                     VNQITSCGADAGCREIRRINVSAAFFLSSEFQETGLLVQNAHRAAFGTRPLYGTFMRD
                     AQAVSRGVIIGQPGALEQLEANKQAYFNELVTRPEFVTKYPASLTNEQYVDNLLASAG
                     LSPSNFVVNLTNAQENPPANPTTSTGAFRPRSFGTATFSFNNAQTALTVTATVNNIDF
                     TGTQTADNNDNLTNAHIHASATVTPTTNGGVVWGFIGTPFNDNNPNDAVVTPFGSGVG
                     GAFSGKWDAPEGNNTTLAAQLANLLEGRAYINFHTVQFAGGEIRGNFPAMTQLKAALL
                     AGLTAGTDTRATVLRKVAENEYLRQRELNAAFVQMQYFGYLRRDPDTAGFNFWLDKLN
                     SFGGNFIAAEMVKAFITSSEYRQRFGAN"
     gene            8933..9811
                     /locus_tag="VF240_18275"
     CDS             8933..9811
                     /locus_tag="VF240_18275"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX7177209.1"
                     /translation="MIKDLSTHHGPLTDLFGAATTSAEWDACRLTREQLEFFDEHGYV
                     AGVRVFDETQLEVLRAELASLADPAHPGHELFYEFHSNESEDPARVLFHALGAWRVAP
                     AFHDLLWNPRFLRPAAQLLGGPVRFWHDQLFCKPARHGGVVAWHQDYSYWTRTTPLAH
                     LTCWAGLDDSTLENGCVHYVPGSHRWPDLPITGLAGDMDSILSVLEAELREKFRPVAI
                     ELKAGEASFHHPRMLHGSYENRSDKARRATVVNVFRDGVRSASDAPPLAGVPPIPKGE
                     PMGGQFFPLLFDARAE"
     gene            complement(10022..12385)
                     /locus_tag="VF240_18280"
     CDS             complement(10022..12385)
                     /locus_tag="VF240_18280"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX7177210.1"
                     /translation="MFRPEFPRVGARALRLACLMLLLAALYGVAASPLAQAQPRSEYR
                     AFWVDTFNTTLNNHNDVLAVVNNAKAARANAIFAQVRRRGDAWYLNSQQEPPPDFTPI
                     APGFDALQDIIDTAHAEGIEVHAFVIVGAVWNKNPNFAPSATLGPPTNPNHVFNLHGG
                     YNSSTQQIVPGPNNWLTRTLLPDGTPGGAITFQGHRIGADFWIDLGHPAAAAYYADVL
                     THLVNSYNIDGLHLDRIRYPELGVAGQTPTTGANIGYNPTSVERFQLRHGIAVGSPPP
                     AAGNSLWAQWRRDQVTNLVRRVYLNAVAVKPQIKISAALIAFGGGPVTEAQWNSAEAY
                     WRVYQDWRAWTQEGILDIAMPMDYKRENVAAQITQFDQWAEWTKNHQYDRAGVIGQGA
                     FLNSVEGTLRQTRRALAPSAAGASGAGIIYFSMATSNIAVTANPHSLPPGQNTPARPF
                     AEFASGLTTGKSVNGLTQYEDQTLNPTAIFSEAATIPVFQWKANPTKGHLKGFARRAD
                     SSILDTADVTIEDLATHAARTTATDGGGFYGGVDLAPGQYLVKAVLGADTVYSCVANV
                     SAGSVTTADLAPETTAPETTATLSPAAPDGANGWYVSDVAVTLDGTDECSGVAATEYS
                     TDGGVTWTPYPAGGFTISAEGVTTVLYRSTDRAGNVEAARTLTVKLDKSAPTITLSAD
                     PSEIWPPDGRTVNVTLSGQGADAVSGLAGVTYVVTDEYGAPLSIPARTLSGASASWAD
                     SLDVEAQRNGGDTDGRLYRITATLTDAAGHTTTATADVVVLHDRGKN"
     gene            complement(12422..>12759)
                     /locus_tag="VF240_18285"
     CDS             complement(12422..>12759)
                     /locus_tag="VF240_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177211.1"
                     /translation="MGRICRMKKELISDFKSSYPIHPVLSMLIPPDGEGSGLVPAPTS
                     VIGVELLKQTLKNILMVGIKKYRPCRAARPLTARALCHIVPRNDERLSLRSSDCCPVP
                     SLPSARAWV"
BASE COUNT         2130 a         4149 c         4323 g         2157 t
ORIGIN      
        1 ctggtgagcg ggaacgcgat gccgcgccgt ctgagcgcct cgatgatttg catccgcgcg
       61 tcggggtctt tgggaatctg ccggacgagc cgcaggaact cctcgttcgt gagcggagcc
      121 tgctgcgtct gcgcggagac gcgcgagggc gccgctccaa gtagaacaac gcacaggaga
      181 gaaaggagta gggcgggcaa aagtttaaaa aatctcataa aacgaaagct tcttttgcgt
      241 cctctgacgg cgcgcgtgga gaatgccgaa cagttattga tgttccgcca cgcaaaaacg
      301 ttcgatcatg cgtgcgagga tcgagacgca ttcgctcagt tcggagcgcg ggacgaactc
      361 attcgtgcgg tgggcgacgc ggatgtcgcc ggggccgaag acgaccgccg cggctccgag
      421 ttcggccatc tgcggcgcct cggtgccgaa ggagacggcg cccgcgcgcc tccccgtcgc
      481 ctcttcgagc gcgcgcacga gcggcgcgtc ggcgctcgtc tccagaccgc ggtcgaggcg
      541 gatgaccttg agggcgcagt cgaagtcggc gtccgtcgag cgcagctcgt cgatctcgcg
      601 gcggaggagt tcgaggaagt attctggcgt ctcggtaggt atgggacgcc attcgagtgt
      661 gaagcggcac tcgccgggga tgatgttctt ggcggcgccg ccgtggatga tgccgacgtt
      721 gacggtcgtg tgcggcgggt cgaaggcttc gtgagcgcgc cccttgaggt cttccgagat
      781 gcgctcgatg cgcgtgatga gacgggcggc gcgcatgacg gccgaagcgc cgaggtgcgg
      841 gtaggcgctg tgcgcctcgc gcccgcgcag agtgacttcc gccagacagt agcccttgcc
      901 ggcgcgcacg ggttgcaacc ccgtcggctc gccgacgatg gccgcgcgtg ggcgcagggc
      961 gcgcgcgtcc gacagacgct tggcgccgag gcagccgatc tcctcgtcgg ccgtgaggac
     1021 gagtgcgagc gggcgtcgga ggcgcgcaag gtcggtctgt tgaatcgccg tgagggccgc
     1081 cgcgataaag cccttcgtgt cgcaagcgcc gcggccgtag agtttgccgt cgcgctcggt
     1141 gagcgtgagc gcgtccgtcc acgcggggtc gaacggcacg gtgtcggtgt ggccgacgag
     1201 cgccagcccg atttcgtcgt cgtctctaac ctcgcgcggc gcgacggcga ccatctggat
     1261 ttttttgacg ccctgctcgt ccgtgtaggg gaggaggcgc gcgcacagtc ccgccgcctc
     1321 gacgagcggc gcgacgtggc tgatgaactc gacgttcgtg cgcgacgagc gcgtgtcgag
     1381 gcggacgagg cccgccagcg tctcttcgac gttcatgggg acattctacc gcaggcggct
     1441 cagaagattg actcatcgac gcattgattc aatgaaggca gaccgataca cacagtaccg
     1501 cgagcggtag cgagcgggtc tccatgccgc gcacacaccc gctcgctacc gctcgcggta
     1561 ctgttttcta gcggccccag agttttcgtt gcagcgacga gaagcgcgcg acgacttcct
     1621 cctgcgccgg gtggcggcct tcgctgacga cgaggcggcc gccgacggcc gtttcgcgca
     1681 gggccgtgcg cgccatcgag aagacgatgg ccgggagcag atcgtcttcc gccgcgccgg
     1741 cgacggaagg gtcttcaagg tcaatggtga aaaagtcggc ggcgcggccc ggcgcgagtt
     1801 ggcccgcgcc ggcgccgatg ctgtgcgcgc ccgcgacagt ggcgcagtcg aagagacgcg
     1861 cggcgagggc cgggagttgt gacgcctcgt cgtgcgtctc ccggtcttcg ccgtccgcac
     1921 ccccggcggc ggcgggcggc gcgaggacgt tgcgccggag cttttgcagg cgcaggtggt
     1981 attcgagttc gcgcgcgtct tcgagcaggt cgatctgcgc gtgactgtcg gtgccgagcg
     2041 cgacgcgcac gccgcgcgcg aacatctcgt cggcgggcac gacgccgtcg cccagattgc
     2101 gctccgtcgt cgggcaggcg cagacggtcg cgccggcggc ggcgagcgcc tccgtctctt
     2161 cgggcgtgac gtggacggcg tgaacgccgg tgaatcgaga gtcgagtatg ccgctgtcgg
     2221 cgagcagcgc gacgggcgtg aagccggtct cctcgacgca ggcggcgacc tcggccggct
     2281 gctcggcgac gtgcatgtgg acgcacaacg cgcgcccgcg cgcgaactcg gccacggcgc
     2341 gcgcgtactc gacggggacg gcgcgcacgc tgtgcggggc gacgccggcc cacgcgcgtc
     2401 ttggcgcttc ggccccgtcg ccgttcgacg cttcggcgcg cgcgaggtcg tcgtctaatg
     2461 cctcggcgtt gcggatgaac tccgcggggt cagattcgat gaagcgccgc tggcgctcgt
     2521 tgggcggcgt cgcgtacccg gagcgcgcgt aggcgacgcg cagcagagcg attctcaagc
     2581 ccacgtcgcg ggcggcgcgg actacctctt tggcgagcag gttcgcttcg tcgtaggccg
     2641 cgccctcggg cgcgcggtgg agatagtgaa actcgccgac ggaggtgacg cccgtcagca
     2701 tcatctccat gaaggccatg cgcgaggcgt cgtagatgtc ttcgggggag aggcgcgcgg
     2761 ccgcccgata catcatctcg cgccacgtcc agaaggagtc ggaggcggct gggctgcgat
     2821 actcggtgcg cccgcggatg acgcgctgga aggcgtgcga gtgagcgttg accatgccgg
     2881 gcagcagggc gcggttgcgg agacggacga agcgggtcgc gagataacgg tcggagattg
     2941 tgatgtcgtg ggtctcgccc tcgcgcggct ccccgcccgt gagcgttcgc aacaagctca
     3001 tcggcgacag ggcgacgacg cggccccgcg cgtcgcacgt cagccccacg tcgcggcgga
     3061 agcgaccgtc ggcgtagatt aaatcgggca cccacgtgat gtgagcgccc ggttcggtgt
     3121 tctggtttga agtgctcaag agaagatttt aggggtggag acaagtccac ggcccgtgcc
     3181 ggcgaaacga aattaacatg gatgggcagg atggtcagga tgaagaaaag ataatttcag
     3241 atttgagatt tcagatttca aatcttttca tcctccccat cctgcccatc catgttaatt
     3301 gcttcctgca atgcgacccc gacagtcatt cggagcgtca gcggcggcgc gcgctctgcc
     3361 cttcggcggc gcgcttcttc tgcgacctga tccagtcgtc cacgatgcgc tccagcacgt
     3421 cgagcgggac ggcgcccgag ccgagcacca cgtcgtggaa ctcgcgcacg tcaaacttgt
     3481 cgccgagctg actcttcgcg cgcgcgcgca actccttgaa cttcagctcg ccgatcttgt
     3541 aggcgagcgc ctgtccgggg ttggtgatgt agcggtcgat ctcgttgacg atgtcctgct
     3601 cggtcttggc ggcgttgtcg aggaagtagt cgatggcgcg ctgccggtcc cacttcatgt
     3661 ggtgcatccc cgtatcgacg acgaggcgca cggcgcgcca catctcgtag gtgagctggc
     3721 cgaacttcga gtaggggtcg tcgtagagac ccatgtcctc gccgagtgat tcggcgtaga
     3781 ggccccagcc ttcgacgaag gcggtgtagc cgccgtagcg ccggaactcg ggaatctcgc
     3841 ccagctccat cgcgcgggag atctggaagt gatggccggg cacggcctcg tgcagagaga
     3901 gcgccatcat ctcccacttc gggcggctct ccggcttgta gagattgacg taatagtagc
     3961 cgggccggga gccgtcggcc gcgggctggt tgtagtaggc ggtagtggtg tcgggcgcga
     4021 tcttgtcggg gatggggatg acgccgtagg gcacgcgcgg gatggtcttg aaaactttga
     4081 cgaggtgcgg gtcgatcttc ttcgagatgg cgcggtaggc gtcgagcagt tcttcgggct
     4141 tcttatagta gaactggtcg tcggtgcgca gcttcttgaa gaactccttg tgggttcctt
     4201 tgaagccgac ctgcgtcatg atcttctgca tctcggcgcg gatgcgggcg acttcagaga
     4261 ggccgcgctc gtgtatctgc tcgggcgtca tgccggtcgt cgtgtaggag cgcgcgtagt
     4321 aggcgtaggc cgcgccgccg tcgcgccact gccagatgcc caccttgtcg taggaggcgg
     4381 gcaggtactc cgcgacgaag tattccttga gccggcggta ggccggaacg acgcctgtcg
     4441 agatagtctc actcgctgcg cgcgtcaggc gctcgcgctc ggcggccggg atgtcggcgt
     4501 gaaaattttt gaacggcttg tagaacggac tgtcttcggc gcgggcgacg atctgcttgt
     4561 cgagctgcgc gggcacgcgc tgcatcgtca ccttcggcca gaggatgccc gccgctttgc
     4621 cttcgcgcag cagcgcgatt gactgatcca tgtagttcgg cagcgcgcgc agacgcgcca
     4681 gccagtcgtc gtagtccttg acggtctgga agcgcagttg atcggacagc tcgtccgagt
     4741 tctggacgcc ttcgcgctgg ttgatgggca aaaggtaggc gcggaacttg tgctcctcga
     4801 tggccagctc caaatctttt ttgaagaggt cgtagttcaa ctggtcggcc ggcgacatgc
     4861 gcgcgcgctc aagccgggag aggcgcgcga gcacgtcctg ccggtggcgg tggcggcgct
     4921 cgtaagcctc gacgctgatg tccgtccagc ggtcgttgta gcggagatcg cccaaagagg
     4981 aggcgaacgt cgggtactcc tgcatcgtcc attgccactc ggcgtcgaag atggcgtgga
     5041 gctgacggcc tgcgcccgct tgcccctgtg cggcgccggc gcgggggcgc gggttcgttc
     5101 tctgtgcttt ggtcggcatg gcttgcaaga ctacggcggc gacaatcagg aagaggagcg
     5161 agcacttccg gctagggggc atcgagtaac ctccgtctgt tcgggtgagc gggatgaagc
     5221 gagagacggg gaaggataca atacaagaag ggaaaaaggt aaaagtaaaa aggcaaaagt
     5281 ggggaagccg aggcacgggt atcaacgagc cgccggaggc ggcggaagaa tgtagcccac
     5341 ggcgtgagcc gtgggtaaac gcagaacaaa aatgaacgaa gccccggagg ggcgaaagaa
     5401 tcgcgacgag gctcgtgctc aacgtcgcgg ttctttcgcc cctccggggc ttcggattat
     5461 ctccggcgtc ttgtccccac ggctcacgcc gtgggctaca ttcttccgcc gctttcagcg
     5521 gctcgttgac tgctcactgc tcactgccta ctgttcttca ccactgcaag cggtcgcggt
     5581 actcgcccgc gaggatgaag gagcgaacca tctcggcgga gggcagcccg gtgctgccgt
     5641 gacacccgac agtcccgcgc ggcccgcgaa aaatacgtcg ggcggggcga aatctttttg
     5701 tgggcgcgcg cggcgcggaa gagtcttgac agcgtcgcgc gtcagtaata gagtctcggg
     5761 cactccccgc agcgggtttc gctcttcatg ctcaaaattc tgcgtggacg gcccggcgag
     5821 tcgcccgcgc tccaacctcg cctgtggggc acgcctcgcc ccttcgtgaa aatgtctccg
     5881 caccgcgcgt acacgtgctt cgcgcgcggc cagaaacttc aggcgtctcc cggcgcgcgg
     5941 ccccgactac acggtcgcag gcggcgacgg cagacatcag gacgaatctc tttccaaaac
     6001 gcacctaagg agaatccaga tgctccacct tcttcgctcc gtcaagtttc gcgccagaaa
     6061 gtcggccggc ttcgcgctgg cctggttgtt gatcctctcg gccctgcccg tcgctccggg
     6121 cgccgcggcg cgcacgccga agccgggcgc tggcgcgcaa gaccccgcgc cggcccagca
     6181 gatacccgtc atcgcgccgg gcagcgccta ccggcaggcc aacctcgtct ccgacgtgcc
     6241 cggcctcgcc ttcgtcctcg acccgctgct cgtcaacccg tggggcatct cgctgaccgc
     6301 gaccagcccc ttctgggtcg ccaacaacgg gacgagcacg acgcagctca tccgcggcga
     6361 cgtgggcggc gcgcccgtcg tcctcaaccc tagcccgcag acgatcacca tcccgggcgg
     6421 gctgccgacg gggacggtcg ccaacggtgt ggcgacggaa ttcgtcctcc ccggcgcgtg
     6481 cgccagcgcg ccgtgcgggg cgaacttcct cttcgcctcc atcacgggca acatcacggg
     6541 ctgggacccc aacgccccgg ccgccggctc gaccaccgcc gtcatcgcgg cgagccaccc
     6601 cggccacgtc tacaccggcc tcgccatcgc caacaacggc tcggggaact tcctctacgc
     6661 cgccgacttc gccaacggca ggatcgacgt tttcaactca agcttcgccc tccagcccgc
     6721 ggccagcttc cccttcgccg acccgaccat cccgacgacg atggggaaca cctaccaccc
     6781 cttcaacatt caggccatcg gcagttcgct ctacgtcgcc tacgcgaagg tcgggccggg
     6841 cggcctcgac gtggagggcg tcggcaacgg cttcgtccgc cgcttcaacg ccaacggcgt
     6901 gcgcgacctg accttcggca tcaacaacgg cgcgctcaac tcgccgtggg gcatcacgca
     6961 ggctcccgcc accttcggca tctttggcgg cgccctgctc gtcggcaact tcggccgcgg
     7021 caacccgagc atccacgcct acaacccgac gaccggcgcc ttcctcggca cgcttcagga
     7081 tgagagcggc aacggcatcg tgattgacga gctgtgggac ctcaagtttg gcaacggcgg
     7141 cgcgggcggc gaccccaaca cgctctactt cgccgccggc atcggccatg aggagcacgg
     7201 cctcttcggc tccctcaagc cgaccacggc ctcggccact tcgctcatcc agttcagcac
     7261 ggacgactac gccatcagcg aaaactccgg ccacatcgac atcaccgtga cgcgcaacgg
     7321 cgacgtctcc ggctcggcca ccatcaacta caacaccttc gacgagtcgc agcccggcca
     7381 cgcctcgcag aagtcggact acgagatcgc cctcggcacg ctccacttcg cccccggcga
     7441 gacctcgaag accttccgca tcctgatcgt ggacgacaaa gacttcgcgc ctttccccgg
     7501 tgggaatgag ggcaacgagg tggtcagcct cgccctctcg aacccgaccg gcgcgggcgt
     7561 cggcctcggc agcccgaacg tggtggagtt gacgataatc gacgatgacg cgttgctgcc
     7621 gacgacgaat cccatcgacg acgcgacctt cttcgtccgc cagcactacc tcgacttcct
     7681 caaccgcgag cctgaccccg ccggcctcgc cttctgggtg aaccagatca cctcgtgcgg
     7741 cgccgatgcg ggctgccgcg agattcggcg catcaacgtc agcgcggcct tcttcctctc
     7801 cagcgagttc caggagacgg gcctcctcgt ccaaaacgcg caccgcgccg ccttcggcac
     7861 tcgcccgctc tacgggacgt tcatgcgcga cgcgcaggcc gtcagccgcg gcgtcatcat
     7921 cggccagccc ggcgcgctcg aacagcttga ggccaacaaa caggcttact tcaacgagct
     7981 cgtcacacgc cccgaattcg tcacgaaata cccggcgtcg ctgacgaacg agcagtacgt
     8041 tgacaacctg ctcgcctcgg cggggctttc gcccagtaac ttcgtcgtca acctcacgaa
     8101 cgcgcaggaa aatccgccgg ccaaccccac cacctcgacg ggcgccttcc ggccgaggtc
     8161 cttcggcacg gcgaccttca gcttcaacaa cgcacagacg gccctgacgg ttacggccac
     8221 ggtcaataac atcgacttca ccggcacgca gacggccgac aataacgaca acctgacgaa
     8281 cgcacacatc catgccagtg cgaccgtcac gccgaccacc aacggcggcg tcgtctgggg
     8341 cttcatcggc accccgttca acgacaacaa cccgaacgac gcggtggtga cgccgtttgg
     8401 ctccggggtc gggggtgcct ttagcggcaa gtgggacgcg cccgagggca acaacacgac
     8461 gctggccgcc cagctcgcga acctcctgga gggtcgcgcc tacatcaact tccacaccgt
     8521 ccagttcgcc ggtggcgaga tccgcggcaa cttcccggcg atgacccagt tgaaggccgc
     8581 actcctggcg ggcctgaccg ccgggaccga tacgcgcgcc acggtgctgc ggaaggtggc
     8641 cgagaacgag tatctgaggc agagggagct aaacgcggcc ttcgtccaga tgcagtactt
     8701 cggctacctg cgccgcgacc cggacacggc cggcttcaac ttctggctgg acaagctcaa
     8761 cagcttcggc ggcaacttca tcgccgccga gatggtcaag gcgttcatca cgtcgagcga
     8821 gtaccgccag cggttcggcg ccaactagac cgccctcgaa agggcgcata agcagtgacg
     8881 ggtgacgagt tgaagacggc ttccggctcg tcacccgtca cgtttgattt ccgtgattaa
     8941 agacctgtcg acacatcacg gccccctgac agacctcttc ggcgcggcca cgacgagcgc
     9001 ggaatgggac gcgtgccggc tgacgcggga gcagttggag ttcttcgacg agcacggcta
     9061 cgtggcgggc gtgcgcgtgt tcgacgagac gcagttggaa gtcttgcgcg cggagctggc
     9121 tagcttggcc gaccccgcac accccggcca cgaactcttt tacgagtttc actcgaacga
     9181 atcggaagac ccggcgcgcg tgctcttcca cgccctcggc gcgtggcgcg tggcgcccgc
     9241 cttccacgac ctgctctgga atccacgctt cctgcggccg gccgcgcaac tcctcggcgg
     9301 ccccgtgcgc ttctggcacg accagctctt ttgcaagccc gcgcgccacg gcggcgtcgt
     9361 tgcctggcat caggactact cctactggac acgcaccacc ccgctcgcgc acctcacctg
     9421 ctgggccgga ctcgacgact cgacgcttga gaacggctgc gtccactacg tgcccggcag
     9481 ccaccgctgg cccgacctcc ccatcactgg actcgccggt gacatggact cgatcctgtc
     9541 cgtgctcgaa gcggagctgc gcgaaaagtt tcggcccgtg gcgattgaac tcaaggcggg
     9601 cgaggcgtcc ttccaccacc cgcggatgct ccacggctcc tacgagaacc ggagcgacaa
     9661 agcgcgccgc gccacggtcg tcaacgtctt tcgcgacggc gtgcgctccg cctccgacgc
     9721 gcccccgctc gccggcgtgc ctcccatccc gaagggcgaa ccgatgggcg gacagttctt
     9781 cccgctgttg ttcgacgcgc gcgccgagta aggcaaaagt aaaaaggcaa aaggcaaaag
     9841 tgacatagcc gaagcacggg cgtcagcgag ccgccggagg cggcggcagc agtgttgccc
     9901 acggcgcaag ccgtgggtta accgtcaaca aaaatcaaag ccccggaggg gcgaaagaaa
     9961 ttatgaggcg tcaacctcaa atatctttcg cccctccggg gcttccttag cctgcccgcc
    10021 gctaattctt cccgcggtcg tgcaggacga cgacatcggc ggtggccgtg gtggtgtggc
    10081 cggccgcgtc cgtgagcgtc gccgtgatgc ggtagagccg tccgtccgtg tcgccgccgt
    10141 tgcgctgcgc ctcgacgtcg agcgagtcgg cccaggaggc ggaagcgccc gagagcgtgc
    10201 gcgcggggat ggagagcggc gcgccgtact cgtccgtgac gacgtaggtc acgcccgcga
    10261 ggcccgacac cgcgtccgcg ccctggccgc tgagggtaac gttgacggtg cgcccgtccg
    10321 gcggccaaat ctccgacggg tcggccgaga gcgtaatggt cggggccgac ttgtcgagct
    10381 tgacggtgag tgtgcgcgcc gcctcgacgt tgcccgcgcg gtcggtcgag cgatagagga
    10441 cggtcgtcac gccctcggcc gagatggtga agccgcccgc cgggtagggc gtccacgtca
    10501 cgccgccgtc ggtcgagtac tcggtcgcgg cgacgccgga gcactcgtcc gtcccgtcga
    10561 gcgtgacggc cacgtcggag acgtaccagc cgttcgcgcc gtcgggcgcg gcggggctga
    10621 gtgtggcggt cgtctcgggc gcggtcgtct cgggcgcgag gtcggcggtc gtcaccgagc
    10681 cggctgaaac attcgccaca cacgagtaga cggtgtcggc gccgagcacg gccttgacga
    10741 gatactgacc gggcgcgagg tctacgccgc cgtagaagcc gccgccgtcg gtggcggtcg
    10801 tgcgcgcggc gtgcgtcgcg aggtcttcaa tcgtcacgtc ggccgtgtcg aggatggacg
    10861 agtcggcgcg ccgggcgaat cccttcaggt ggcccttcgt cgggttggcc ttccactgga
    10921 agaccggaat cgtcgccgcc tccgagaaga tagccgtggg gttcaacgtc tgatcttcat
    10981 actgcgtcag gccgttgact gacttgccgg ttgtcagccc cgaagcgaat tcggcgaagg
    11041 ggcgcgcggg cgtgttctgc cccggcggca gggagtgcgg gttggccgtg acggcgatgt
    11101 tcgaggtggc catcgagaag tagatgatgc cggcgccgct cgcgccagcg gccgagggcg
    11161 cgagcgcgcg gcgcgtctgc cggagcgtgc cctcgacgga gttgaggaag gcgccctgtc
    11221 cgatgacgcc ggcgcggtcg tactggtggt tcttcgtcca ctcggcccac tggtcgaact
    11281 gcgtgatttg cgccgcgacg ttctcgcgct tgtagtccat cggcatcgcg atgtcgagga
    11341 tgccctcctg cgtccacgcg cgccagtcct gatagacgcg ccaataggct tcggccgagt
    11401 tccactgcgc ctcggtcacg gggccgccgc cgaaggcgat gagcgcggcg gaaatcttga
    11461 tctgcggttt gacggcgacg gcgttgagat agacgcgccg gacgaggttt gtgacctgat
    11521 cgcgacgcca ctgcgcccag agcgaattgc cggccgcggg cggcggcgag ccgacggcga
    11581 taccgtggcg gagctggaaa cgttcgacgc tcgtcgggtt atagccgatg ttggcgccgg
    11641 tcgtgggcgt ctgccccgcg actccgagtt ccgggtagcg gatgcggtcg aggtgcaggc
    11701 cgtcgatgtt gtaactgttg acgaggtgcg tgagcacgtc ggcgtagtaa gcggccgccg
    11761 ccgggtggcc gaggtcgatc cagaagtcgg cgccgatgcg gtggccctgg aaggtgatgg
    11821 cgccgcccgg cgtgccgtcg gggagcagcg tgcgcgtcag ccagttgttc ggccccggca
    11881 cgatctgctg cgtcgaggag ttgtagccgc catggaggtt gaagacgtgg ttcgggttgg
    11941 tcggcggccc gagcgtcgcc gacggcgcga agttcgggtt cttgttccag accgcgccga
    12001 cgatgacgaa ggcatgcacc tcgatgcctt cggcgtgggc cgtgtcgatg atgtcctgaa
    12061 gcgcgtcgaa gccgggggcg atgggcgtga agtcgggcgg cggctcctgc tgcgagttca
    12121 agtaccacgc gtcgccgcga cggcgcacct gcgcgaagat ggcgttggca cgggcggcct
    12181 ttgcgttgtt gacgaccgcc agcacgtcgt tgtggttgtt gagcgtggtg ttgaaagtgt
    12241 caacccagaa ggcgcggtac tcgctgcgcg gctgcgcctg cgcgagcggc gacgcggcga
    12301 cgccgtagag cgcggccagc agcagcatca ggcacgcaag ccgaagcgca cgcgcgccga
    12361 cgcgagggaa ttcgggacga aacatagaga tttggtctcc ttagaggaaa ttgcttgagg
    12421 ctcagaccca agcacgagca gatggcagag agggtacggg gcaacagtcc gaactacgaa
    12481 ggctaagccg ttcatcgttt cgcgggacga tatgacaaag cgcgcgcgca gtcaacggac
    12541 gcgccgcgcg gcagggtcga tattttttta taccgaccat aagaatattt tttagagtct
    12601 gtttcaaaag ctcaacgccg atcacgcttg taggggcagg gacaagtccg ctgccctcgc
    12661 cgtcgggagg aattaacatg gataggacag gatgaatagg ataagaagat ttgaaatctg
    12721 aaatcagctc tttcttcatc ctgcatatcc tgcccatcc
//
LOCUS       DASVES010000443         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1248650, whole genome shotgun sequence.
ACCESSION   DASVES010000443 DASVES010000000
VERSION     DASVES010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248650"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VF240_18290"
     CDS             <1..301
                     /locus_tag="VF240_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177212.1"
                     /translation="NVSKVGAGFLQDVRSWIWMPTRVEFDLSLDGASYERAASVEPDV
                     PVRDESVMVKDFVREFARRRARYLRVRAYNYGVIPAWHPGAGDSAWVFVDEIIVE"
     gene            complement(477..1388)
                     /locus_tag="VF240_18295"
     CDS             complement(477..1388)
                     /locus_tag="VF240_18295"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7177213.1"
                     /translation="MIKNIIRFPLTALLLLTLSAISAQAQGKSAKAGKQVAVKTAAAT
                     TTAPADDPEQFRKDFIKAAEEHKSSLKALAASYEGSIEKLNAQHDKLKELYTDGIIAR
                     RDLEASETALVEARSRVEVTLKELAAVEATLEAALKPPAPADMTVAAGPRVDVRWTTG
                     NNKIDGLIRHYGNLYGVDPYLVYCIMHQESRFGSGAVSYKGAQGLMQLMPGTAARYGV
                     TNSFDPAQNISGGTRYLRDLLRLFNGRVDLVLAGYNAGEGAVMKYGRRVPPYNETQNY
                     VRIIGSRYAKGTGVQLTSKTSTPKGGK"
     gene            complement(1966..2664)
                     /locus_tag="VF240_18300"
     CDS             complement(1966..2664)
                     /locus_tag="VF240_18300"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7177214.1"
                     /translation="MINARKANERGHFNFGWLDTYHTFSFGDYYDPRQQGFRSLRVIN
                     EDRVHPGRGFPTHPHRDMEIITYILSGALEHRDSMGTGSVIRPGDVQRMSAGTGVTHS
                     EANPSPTEPVHLLQIWILPRERGIRPSYEQKAFTEREKRARLRLVASADGQDGSVRIH
                     QDASLHAALLAPGEEVTHALAPGRAAWVQVARGAVALNGQPLGQSDGAAVSDEAELVI
                     AGVEPSEILLFDLA"
     gene            2788..3882
                     /locus_tag="VF240_18305"
     CDS             2788..3882
                     /locus_tag="VF240_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177215.1"
                     /translation="MLITLLIALILPSTQTPTTSARWEQQTSGTTVRLRGVSAVSASV
                     AWASGQGGAYARTTDGGRTWRARVVPGAEALDFRDVDAFDASTAYLLAIGEGDKSRIY
                     KTTDGGANWVLQFRNDDPSAFFDAMAFWDRDHGIAMSDPVQGRFLIITTSDGGRNWRP
                     IPPAGMPPALEGEGGFAASGTCIAVQGKSNVWFGTGGPSGARVFRSTDGGRTWTVAPA
                     PLAAGKAAGVFSLVFWDARRGVAVGGDYTKEKDAEGSAATTDDGGRTWKAVAGDARPS
                     GYRSAVARASAATRPTLVAVGPSGTDRSLDGGRDWTPLGAEGFHAVSFAADGAGWAVG
                     ENGIVARYVGPAPGAQRRARIQSNKSRKSP"
BASE COUNT          689 a         1251 c         1343 g          739 t
ORIGIN      
        1 gaacgtgtcg aaggtcggcg cagggttttt gcaggacgtg cggtcgtgga tttggatgcc
       61 gaccagagtc gagttcgatt tgtcgctcga cggcgcgagc tacgagcggg ccgcctcggt
      121 cgaaccggac gtgcccgtgc gcgacgagtc ggtgatggtg aaagacttcg tgcgcgagtt
      181 cgcgcgcagg cgagcgcgct acttgagggt cagggcttac aactacgggg tcatcccggc
      241 gtggcatccc ggtgcgggcg acagtgcgtg ggtattcgta gatgagatta tcgtcgagtg
      301 aggcgcggtg cggtgcccga ggcaagcttg agttgagtgt aggtagagtc ggactcaccg
      361 cagaggcgca gaggacagga gaagaaggca aaagtaaaaa ggcaaaaggc aaaagtgaga
      421 aggcaggtcg cctcttcact tttgcctttt gcctttttac ttttgcctta cctgagctac
      481 ttgccgccct tcggcgtcga agtcttcgac gtgagctgca cgcccgtgcc cttggcgtag
      541 cgcgagccga tgatgcgaac gtagttctgc gtctcgttgt agggcgggac gcggcggcca
      601 tacttcatca cggccccctc gcccgcgttg tagccggcga gcacgaggtc aacccggccg
      661 ttgaagagcc ggagcaggtc gcgcaggtag cgcgtgccgc ccgagatgtt ctgcgccggg
      721 tcgaaggagt tggtcacgcc gtagcgcgcg gccgtccccg gcatcaactg catcagcccc
      781 tgcgcgccct tgtagctcac cgcgcccgag ccgaagcgcg actcctggtg catgatgcag
      841 tagacgaggt agggatcgac gccgtagagg ttgccgtagt ggcggatgag gccgtcgatc
      901 ttattgttcc cggtcgtcca cctgacatcg acgcgcggcc ccgccgcgac tgtcatgtcg
      961 gccggcgcgg gcggcttgag ggccgcctcc agcgtcgcct cgaccgcggc cagctccttg
     1021 agcgtaacct cgacgcggct gcgcgcctcg acgagcgccg tctcgctcgc ctccagatcg
     1081 cggcgggcga tgatgccgtc ggtgtaaagc tccttcagct tgtcgtgctg cgcgttgagc
     1141 ttttcgatgc tcccctcgta ggaggcggcc agcgccttga ggctcgactt gtgttcctcg
     1201 gcggccttga tgaagtcctt gcggaactgc tcggggtcgt cggccggggc cgtcgtcgtc
     1261 gccgccgccg tcttcacggc gacttgcttg ccggctttgg cagacttgcc ctgcgcctgc
     1321 gcggagatgg ccgaaagcgt caggaggagg agtgcggtca gggggaatcg aataatgttt
     1381 tttatcatca cttaatcaga ggaaccagca atcgtcgccg gtgaatcgtc cagaaacttt
     1441 tacgtgcacg gggccggtaa gttccggccg acaagcttta aaacatgacc gttgacagtg
     1501 aaaatcgccc cggccggggc tttttaaagt gggcgacggg cgcggcccgt cgattgtgcg
     1561 ttctgtggaa gttttgtcag aggactcttt gaactccgtc tcttctcgtc gccgccaaat
     1621 ttttcaacct tcaaggggct aaatacaacc aacctttaga cgttatagcc ccctcgccgg
     1681 ttgcacggac ggccgccgcg acccgtgtaa tttctgtagg ggcagggctt gcccgctacc
     1741 cgcgcattag ggacaagagg tttagacagg atttacagga ctgacaggat caataggatg
     1801 agttaatttg agattggaaa tctgaaatct gaaatctgta atttcaaatc tgcttcgcct
     1861 tcatcctgtc agtcctttca gtcctgtcta aacctcttaa ttcctccgta gcacggggcc
     1921 gcagacttgc acccgccccg acaggtttga aacgcgctga aatgttcagg cgaggtcgaa
     1981 gaggagtatt tctgacggct cgacgccggc gatgacgagt tcggcctcgt cgctcacggc
     2041 cgcgccgtcg gactgaccga ggggctggcc gttgagcgcg acggccccgc gggcgacctg
     2101 cacccaggcg gcgcgtcccg gcgcgagggc gtgcgtcacc tcctcgccgg gcgcgagcag
     2161 ggcggcgtgg aggctcgcgt cctgatggat gcggacggag ccgtcctgcc cgtcagccga
     2221 cgcgacgagg cgcaggcgag cgcgcttctc acgttcggtg aaagccttct gctcgtagct
     2281 cgggcggatg ccacgctcgc gcggcagaat ccagatttgg aggagatgaa ctggctcggt
     2341 gggcgacggg ttggcctcac tgtgcgtgac gccggtgccg gcgctcatcc gctgcacgtc
     2401 gccggggcgg atgacggagc cggtgcccat gctgtcgcgg tgctcaagcg cgccgctcaa
     2461 gatgtaagta ataatctcca tgtcgcggtg cgggtgcgtg gggaagcctc ggccgggatg
     2521 cacgcggtct tcgttgatca cgcgcagcga gcggaagccc tgctggcgcg ggtcgtagta
     2581 gtcgccgaag gagaaggtgt ggtatgtgtc gagccagccg aagttgaagt ggccgcgctc
     2641 gttggccttg cgggcgttaa tcatttgcgt gatgcctccc tgtctcgcac aagggtagag
     2701 catcgcgtct gaaatttcat ctcgcgcgcg cctcggcaca cttgtcaaag tcggcggcga
     2761 tgcgtttaaa tccgaccccc gaacagtatg ctcatcacac ttctcatcgc gctcattctt
     2821 ccttccacgc agacgccgac aacaagtgct cgctgggagc agcaaacgag cggcacgacc
     2881 gtgcgcctgc gcggcgtgag cgccgtgagc gcgagcgttg cgtgggccag cggacagggc
     2941 ggcgcctacg cgcgcacgac cgacggcggg cgcacatggc gcgcaagggt ggtgcccggc
     3001 gccgaggcgc tcgactttcg cgacgtggac gccttcgacg cctcgaccgc ctacctgctc
     3061 gccatcggcg agggcgacaa gtcgcgcatc tacaaaacga ccgacggcgg cgctaactgg
     3121 gtcttgcagt tcaggaacga cgacccgtcc gccttcttcg acgcgatggc cttctgggat
     3181 cgcgaccacg gcatcgccat gagcgacccc gtgcagggtc gcttcctcat catcaccacc
     3241 tcggacggcg ggcgcaactg gcggcccatc ccgcccgcgg gaatgccccc ggcgctcgaa
     3301 ggcgaggggg gcttcgccgc cagcggcacg tgcatcgcgg tgcagggcaa gtcgaacgtc
     3361 tggttcggca cgggcggccc ctccggcgcg cgcgtcttcc gctcgaccga cggcggacgc
     3421 acctggacgg tcgcaccggc cccgctcgcg gcgggcaaag cggcgggcgt cttctcactc
     3481 gttttttggg acgcgcgccg cggcgtcgcc gtcggcggtg attacaccaa agagaaagac
     3541 gcggaaggca gcgcggccac gaccgacgac ggcgggcgga cgtggaaggc cgtcgccgga
     3601 gacgcgcgcc cgtcgggcta tcgctccgcg gtcgcgcgcg cgtccgccgc gacacggccg
     3661 acgctcgtcg ccgtcgggcc ttcgggcacc gaccgctcgc tcgacggcgg ccgcgattgg
     3721 actccgctcg gcgctgaggg cttccacgcc gtgagtttcg ccgccgacgg cgcgggctgg
     3781 gccgtcggcg aaaacggcat cgtcgcacga tacgtcggcc ccgcacccgg cgcacaaaga
     3841 cgcgcacgaa ttcagtcaaa caaatcccga aaatctccct aacgcctttc tttgcgcctt
     3901 tgcgtgagac ttccaccgtc caacaataat aagtctcacg caaaggcgcg ggaggcgcaa
     3961 agacaatacc ctgaacatca tgaggtcaac aggcgaatca agcgggtact gacggccgcg
     4021 tc
//
LOCUS       DASVES010000444         5634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1592060, whole genome shotgun sequence.
ACCESSION   DASVES010000444 DASVES010000000
VERSION     DASVES010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592060"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="VF240_18310"
     CDS             <1..1776
                     /locus_tag="VF240_18310"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX7177216.1"
                     /translation="APRNYRQIRRAEELEPLVRKLWDTDHWSFAVSDSSPAGAGQQAS
                     VHDSQRPTGFAISTAPHTSAFVNFEEFEGGVESVVEMLRDVLTNGLLEKSVHDLKRAA
                     ALLAPFGLDPEGVTDDTLLAAYLLDPVRSRYELADLAREAVGAEGWSQVPDESWTEEQ
                     WRTAETADFTAQVADVLHGRILEQGLEGIYTEIELPLAPLLYRMEHAGLRVDPKVLGD
                     LSALFSKELEKLTTQIYKAAGREFKIGSPKQVGEVFEELNISTGRKTSTGQVSTSRAV
                     LDELAQSYELPRLIIEYREMDKLKGTYTDALPELIGPDGRIHSQLNQTVTATGRLSSS
                     NPNVQNIPIRTELGRRIRRAFVPDKGNVFVSADYSQLELRLLAHITRDEHMLEAFQKG
                     EDIHERTARLVFGAKTKEDLKEKRRFAKIVNFAIAYAVEPFGLSNRVGISRKEAKQVI
                     EDYYATYTGVRRFMDEVPVVAREKGFVRSIYGRIRPIPGINDRNANVRFRAEREAINM
                     PIQGSASDIVKIAMLRVDEALRGEGLKSQLIMQVHDELLIEAPKQEAERASAILKREM
                     ERAVELDVPLEVEIGTGNNWMDVKK"
     gene            1873..3735
                     /locus_tag="VF240_18315"
     CDS             1873..3735
                     /locus_tag="VF240_18315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177217.1"
                     /translation="MPDEKKSGRTGYDRLGGGGEGESRRPSPPTPDKGRAAGAVAERG
                     VGGVSLARTLVPLLVGFVLLIGLVFGLGTLSDRQLSAVSFRSADREGAATETLNRLNR
                     LRLALSRLDNEARSRAQAEARGGLRPPGDFPLGRARDEFKEVLPPFNALPLAQTEKGL
                     VLRADIDKYIEITKDPRRYSLEGFDSNRSIEQRLGALYDEASAARQSIREDSYDELDR
                     ARRQINFLKWLAVLTGLFVAAATTLEVLRRFGQLRRSFEEVRRERQFSRQMLEGMVSA
                     IAAVDGHDHLRSANASFFRIFPEARLGESLHDSVEAPESLKLLASATSTRVHTATYRG
                     RWRLAHEEREHAFDVYSSPLEIEGEPGQILTLVDVTEAAESERELRRREALTAVGQAA
                     AQLAHEIKNPLGSIRLGVAMLRDMSREREAITTIDLVERGIEHLSKLTSDVTQFSRQR
                     QLRLAEVELHELIDESLELVADRVQDKATPIEREFSDEQIRATWDEDQLRQVFVNLIA
                     NAVDASPPRRPVTITTSRVERSVGTGQDGTRGGRAQFARIEVADSGAGMDEAVSRRVF
                     EPFFTTKKKGTGLGLAIAKQIVEAHGGYITVASQPGEGTRFTVELPFSSQAAEV"
     gene            4009..4893
                     /gene="sucD"
                     /locus_tag="VF240_18320"
     CDS             4009..4893
                     /gene="sucD"
                     /locus_tag="VF240_18320"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7177218.1"
                     /translation="MSVLVDKNTRLVVQGVTGKEGTFHTRQMVEYGTNVVGGVTPGKG
                     GTTHEGIPVFNTVAESVSEAGANASVIYVPPAFAADAIMEAADAGLPLVVCITEGIPV
                     LDMVKVREYLSHKPTRLVGPNCPGVISPGKCKIGIMPGHIHREGAIGVVSRSGTLTYE
                     AVGQLTALGLGQSTAIGIGGDPIVGTTHLQALALFQDDPETEGIVMIGEIGGAAEEEA
                     AAFAKDNVTKPIVAFIAGQTAPPGRRMGHAGAIIAGGKGTAAEKMRALEEAGIRVVHS
                     PADIGQAMKDALGSRAAA"
     gene            4914..5363
                     /locus_tag="VF240_18325"
     CDS             4914..5363
                     /locus_tag="VF240_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177219.1"
                     /translation="MSSEDRLTRAEEIIANLAVVARDANKRLSAAEGDIRSIDGRLDK
                     LVEISQGFDERLDTLADLQANSERKIAALADAQIHTEAALVRLSEAQGRTEEALVRLS
                     EAQGRTEEALKGLSMKMSELAAAQAHSDQRLDALIDFVQGADERPQS"
     gene            5386..>5634
                     /locus_tag="VF240_18330"
     CDS             5386..>5634
                     /locus_tag="VF240_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7177220.1"
                     /translation="MSEQTGNLTFGIIKPDAVRAGKTGAVLQRITDGGFRVRAMKLIH
                     MTMAEAEGFYAVHRERPFFGELTEFMSGGPCVVLALERD"
BASE COUNT         1073 a         1918 c         1789 g          854 t
ORIGIN      
        1 gccccgcgca actatcgaca gattcgccgg gccgaagaac tggagccgct cgttcgcaag
       61 ctctgggaca cagaccactg gagcttcgcc gtctccgatt cctccccggc cggcgcgggt
      121 caacaggctt ccgtccacga ctcacaacgc ccgaccggct tcgccatctc gaccgcgccg
      181 cacacgtccg ctttcgtcaa cttcgaggag ttcgagggcg gcgtcgaatc ggttgtcgaa
      241 atgctgcgcg acgtgctaac gaacggattg ctggagaaga gcgtccacga cctcaagcgc
      301 gccgccgcgc tgctcgcgcc cttcggctta gaccccgagg gcgtcaccga cgacacgttg
      361 ctcgccgcct acctgctcga ccccgtccgc agccgctacg agctggccga tctggcgcgc
      421 gaggccgtcg gcgcggaagg atggtcgcaa gtcccggacg agagctggac ggaagagcag
      481 tggcgcacgg ccgagacggc cgacttcacc gcgcaggtcg ccgacgtgct ccacggccgc
      541 atcctcgaac agggcctcga aggcatctac acggagatcg agctgccgct cgccccgctc
      601 ctctatcgca tggagcacgc cggcctgcgc gtcgatccca aagtgctcgg cgatttgtcg
      661 gccctcttct cgaaggagtt ggagaagctc accacgcaga tttacaaagc cgccgggcgc
      721 gagttcaaaa tcggctcgcc caaacaggtc ggcgaagttt ttgaagagct aaacatcagc
      781 accggacgca agacttcgac gggtcaggtc tcgacgagcc gcgccgtcct cgacgagctg
      841 gcgcaaagct acgagttgcc gcgtctcatc atcgagtacc gcgagatgga caagctcaag
      901 ggcacctaca ccgacgcgct gcccgaactc atcggccccg acgggcgcat ccacagccag
      961 ctcaaccaga ccgtcaccgc caccggaaga ctctcatcat cgaatccgaa cgtccagaac
     1021 atccccatcc gcaccgaact cggccggcgc atccgccgcg ccttcgtccc cgacaaaggc
     1081 aacgtcttcg tctcggccga ctactcgcag ttggaactcc gcctgctcgc gcacatcacg
     1141 cgcgatgagc acatgctcga agccttccag aagggtgagg acatccacga gcgcacggcc
     1201 cgcctcgtct tcggcgcgaa gacaaaggaa gacctcaagg agaagcgccg cttcgccaag
     1261 atcgtcaact tcgccatcgc ctacgccgtc gagccgttcg gactgtcgaa tcgcgtcggc
     1321 atctcgcgca aagaggccaa gcaggtcatc gaagactact acgcgaccta cacgggcgtg
     1381 cgccgcttca tggacgaggt gcccgtcgtg gcgcgcgaga aaggattcgt ccgctccatc
     1441 tatgggcgca tccgccccat ccccggcatc aacgaccgca acgccaacgt ccgctttcgc
     1501 gccgagcgcg aagccatcaa catgccgatt caaggctccg ccagcgacat cgtcaagatc
     1561 gccatgctca gggttgacga ggcgctgcgc ggcgagggtc tgaagtcgca gctcatcatg
     1621 caggtacacg acgagctgct catcgaagcc cctaagcagg aggccgaacg cgcgtcggcc
     1681 attctcaagc gcgagatgga acgcgccgtc gaactcgacg tgccgctcga agtcgaaatc
     1741 gggacgggca acaactggat ggacgtgaag aaatagtgat aagtgatgag tgataagtga
     1801 taagaaggaa gaagacgacc cgctttctct ttcttatcac ttatcactca tcacttatca
     1861 cttccttgag ttatgcctga cgagaagaag agcgggcgga cgggctatga ccggcttggc
     1921 gggggcgggg agggcgagtc gcgccgcccg tcgccgccaa cgccggacaa gggaagggcg
     1981 gcgggggccg tcgccgagcg cggggtgggc ggcgtgtcgc tggcgcgcac gctcgtgccg
     2041 ctgcttgtcg gcttcgtcct gctcatcggg ctggtgttcg gactcggcac gttgagcgac
     2101 cggcagttga gcgccgtcag cttccgctcg gccgaccgcg agggagcggc caccgaaacc
     2161 ctcaaccgtc tcaaccgcct ccgcctcgcc ttaagccgcc tcgacaacga ggcgcgctcg
     2221 cgcgcgcagg ccgaggcccg cggcggcctg cgcccgcccg gcgacttccc tctgggccgc
     2281 gcgcgcgacg agttcaaaga agtgctgccg cccttcaacg ccctgccgct cgcgcagacc
     2341 gagaaggggc ttgtactgcg cgccgacatc gacaagtata tcgagatcac aaaagaccca
     2401 cggcgctaca gcctcgaagg cttcgacagc aaccgcagca tcgagcagcg cctcggcgca
     2461 ctctacgatg aggcgagcgc cgcgcggcag tccatcaggg aagactccta cgacgaactc
     2521 gaccgcgcgc ggcgacagat caacttcctc aagtggctcg cggtgttgac gggcctgttc
     2581 gtcgccgccg cgacgaccct ggaggtgctg cgccgcttcg gacagttgcg ccggagcttc
     2641 gaagaggtca ggcgcgagcg gcagttcagc cggcagatgc tcgaaggcat ggtctcggcc
     2701 atcgccgccg tggacgggca cgaccacctc cgcagcgcca acgcttcctt cttccgcatc
     2761 ttccccgagg cgcggctcgg cgagtctttg cacgattcgg tcgaagcgcc cgagtctctg
     2821 aagctgctcg cgtcggcgac ctcgacgcgc gtccacaccg cgacctaccg cggacgctgg
     2881 cgactcgcgc acgaagagcg cgagcacgcc ttcgacgttt actcctcgcc gctggagatc
     2941 gaaggcgagc cggggcagat actcacgctc gtggacgtga cggaggcggc cgagtcggag
     3001 cgcgagctgc gccggcgcga ggcgctgacg gccgtcggtc aggccgccgc gcagctcgcc
     3061 cacgagatca agaacccctt ggggagcatc cgcctcggcg tcgccatgct gcgcgacatg
     3121 tcgcgcgagc gcgaagccat caccaccatc gacctcgtcg agcgcggcat cgagcatctg
     3181 tcgaagctca cctcggacgt gacgcagttc tcccgccagc gacaactccg gctcgccgag
     3241 gtcgagctgc acgagctgat cgacgagagc ctcgaactcg tcgccgaccg cgtgcaggat
     3301 aaggccacgc caatcgagcg ggagttctcc gacgagcaga ttcgcgcgac gtgggacgaa
     3361 gaccagctcc ggcaggtctt cgtcaacctc atcgccaacg ccgtggacgc cagccctccc
     3421 cggcggcccg tcaccatcac gacctcccgc gtggaacgct ccgtgggcac cgggcaggac
     3481 gggacgcgtg gcgggcgggc gcagttcgcg cgcatcgaag tggccgacag cggcgcgggg
     3541 atggacgagg cggtcagccg acgcgtcttc gagccgttct tcaccaccaa gaagaagggg
     3601 acgggactcg gcctcgccat cgccaagcag atcgtcgagg cgcacggcgg ctacatcacg
     3661 gtcgcgagcc agcccggcga gggcacgcgc ttcacggtcg agcttccgtt ctcgtcgcag
     3721 gccgcggagg tgtagcggcg cgggccggga cagtgattca agttggggat gtggcggaag
     3781 cccgaccgtc agggagggcg tagcgtgcgc cattgtcagc gccctccctg acggtcgggc
     3841 ttccgcctcc acctcgcgct ccggcgccgc ttagagcgcg cctgcggccc tgtgttatac
     3901 ttcgcctcct taagacgcaa taaaaatcgt cggctttgat agtcgttcga ccgcgcgcca
     3961 tgtgtgtccc gaacgtctcg cgaccgaccc aatccgagga gtaaaacttt gagcgtactc
     4021 gtcgataaaa acacgcgcct cgtcgtgcag ggcgtcacgg gcaaagaggg cacgttccac
     4081 acccgacaga tggtcgagta tgggacgaac gtcgtcggcg gcgtcacgcc gggcaagggt
     4141 gggacgacgc acgagggcat ccccgtcttc aacaccgtcg ccgagtcggt cagcgaagcg
     4201 ggcgccaacg cctccgtcat ctacgtgccg ccggcctttg ccgccgacgc catcatggag
     4261 gcggccgatg cgggcctgcc gctcgtcgtc tgcatcaccg agggcatccc cgtgttggac
     4321 atggtgaagg tcagagagta tctctctcac aagccgacgc ggctcgtcgg gccgaactgc
     4381 cccggcgtca tctcacccgg caagtgcaag atcggcatca tgcccggcca catccaccgc
     4441 gagggcgcca tcggcgtcgt ctcgcgctcg gggacattga cctatgaggc cgtcgggcag
     4501 ctcaccgcgc tcggcctggg tcagtccacg gccatcggca tcggcggcga ccccatcgtc
     4561 ggcaccacgc accttcaggc gctcgcgctc tttcaggacg accctgagac cgaaggcatc
     4621 gtgatgatcg gcgagatcgg cggcgcggcc gaagaggagg ccgccgcgtt cgccaaagac
     4681 aacgtcacca agcccatcgt cgccttcatc gccgggcaga ccgcgcctcc ggggcggcgc
     4741 atgggccacg ccggcgccat catcgccggc ggcaagggca ccgccgccga gaagatgcgc
     4801 gccctcgaag aggccggcat ccgcgtcgtc cactcgcccg ccgacatcgg tcaggcgatg
     4861 aaggacgccc tcggctcgcg cgccgccgcc tgagtgggga ggtcggaaac gtcatgtcga
     4921 gtgaagacag gttaacgagg gccgaggaga tcatcgccaa ccttgcggtg gtggcgcgtg
     4981 acgccaacaa gcgtctgagc gcggccgaag gagacattcg cagcatcgac gggcgtctgg
     5041 ataagctcgt cgaaatatca cagggattcg acgagcggct cgacaccctc gcggacttac
     5101 aggcgaactc ggaacggaaa attgccgcgc tcgccgacgc gcagattcac acggaggccg
     5161 ccctcgtccg tttatctgaa gctcaaggac ggacggagga ggccctcgtc cgtttatccg
     5221 aagctcaagg gcgaacggag gaggctctca aaggtcttag catgaaaatg tcagaactcg
     5281 ccgccgcaca agctcactca gatcaaaggc tcgacgcgtt gatcgacttc gtgcagggag
     5341 cggatgagcg gccgcaaagt tgaaacagtt aagttgagga gaacgatgtc agaacagaca
     5401 ggcaatctta ctttcggaat catcaagccc gacgcggtgc gcgctgggaa aacgggcgcg
     5461 gtcttgcagc gcatcacgga cggcggcttc cgcgtccgcg ccatgaagct catccacatg
     5521 actatggctg aggccgaagg cttctacgcc gtccaccgcg agcggccctt cttcggcgag
     5581 ctgacggagt tcatgtcggg cggcccctgc gtcgtgctcg cgctcgaacg cgac
//
LOCUS       DASVES010000445        24277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_838865, whole genome shotgun sequence.
ACCESSION   DASVES010000445 DASVES010000000
VERSION     DASVES010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 24277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24277
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838865"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VF240_18335"
     CDS             <1..1009
                     /locus_tag="VF240_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177221.1"
                     /translation="MKLSRRTTVASALCYSALALAAAAGCNSTPEANRAATNAANAAA
                     TPSPTAQTLSATERPQKIKDQMAQRGEQDQASPVLKIAEPAEGATVSGATVRVRLNLS
                     GDLKGYQPHKDPATQMGNHIHVILDNEPYEAYYELGRPFELRNVAAGKHTLRVFASRP
                     WHESYKNDGSFQMVTFNVSGGGDTTQPTTASNGQRMADNRAPAANTNAAATPAASPSA
                     EGKDFGAKPNAAGDVDHTKPLLTYSRPKGEYKDAEAEAIMIDFWLAHAKLEGDGGEYR
                     VRYSVDGAPPQHLRKWEPIWLKGWTAGKHKVKVELVDRNDTVVQNGGYNSTEREITVT
                     K"
     gene            1171..2274
                     /locus_tag="VF240_18340"
     CDS             1171..2274
                     /locus_tag="VF240_18340"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX7177222.1"
                     /translation="MNEAAETERDAHALPAHYGDARAEYDAVRGRRAGLIDLSAARGR
                     VEVSGAEAVQFLNGMVTNDVKALEDGAWMHAAFPNVQGRLLASVRVIRRGGAYLFDTE
                     AATHAVVLKNLERFTLAGDFRVRDASAGTGMISVQGAGSREVVRAVAGEEAAGLRRGR
                     AAEWEWGGAQTLVLRATHTAEDGFDFVCDAGLAVNLWESLASAGARPVGFDALEVLRI
                     EAGIPRYGVDVDDTNVVLEAALDEAVSFTKGCYVGQEIIARIHWRGHVAKRLAGLVLE
                     DGDAPAAAGAKVFSSDGAREIGRVTSSVYSPRLGRAVALGVVKYDFLSAGTEVRVRDG
                     DGGAGSERAARVAELPLVRGSWYDSAGDGAEDA"
     gene            2271..2600
                     /locus_tag="VF240_18345"
     CDS             2271..2600
                     /locus_tag="VF240_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177223.1"
                     /translation="MSEETPRENSASTPSAQNLAYEQARLAYSIIQTLLEHTRVTGDL
                     VALMARAIDEDTLKALTGTPHWAAYLDSRRALERTRADVEKFAAEWTRMSEETPAPPP
                     SIQKSEG"
     gene            complement(2582..3451)
                     /locus_tag="VF240_18350"
     CDS             complement(2582..3451)
                     /locus_tag="VF240_18350"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7177224.1"
                     /translation="MSSTENFRGVHVGCQGWNYDDWVTPPASARPVFYRRGTRADRML
                     EEYARSFDTVEVDSTFYAIPTESAVEGWRKRTPEGFSFSLKLPQEITHRLALRGGEAR
                     ALLEAFCERARGLREKLAAVLVQLPPQFEATRENFAALSAFLPLLPPDVRFAIEFRDP
                     FWFEEEFVAPLVYGGRPNVSLALVEGPWATRERIWRAAVPVLEVTDFVYVRWMGERDI
                     TRFDVVQRDRGANLERWAEAVERLRERVPSVFAYFSNFYEGHAPASANRLKRLLGQAV
                     VDPGELEDQPSLF"
     gene            complement(3462..4064)
                     /locus_tag="VF240_18355"
     CDS             complement(3462..4064)
                     /locus_tag="VF240_18355"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7177225.1"
                     /translation="MAQGSHVYDRLAADYDGAMRPLERWLLGRLRASALRELPERARV
                     LEVGVGTGANFQFYTEGARGACVEPSREMLERARVRAKRPPGLWLVRGRAEELPFADD
                     VFDAGLATLVFCSVGSPARAFSELRRVVRPGGRVVLLEHVRPEGLLGYVFDAFSKLTV
                     ALCDDHFNRRTAEEARRAGLWVERVEPHWRGVFQIIVCRV"
     gene            4420..7821
                     /locus_tag="VF240_18360"
     CDS             4420..7821
                     /locus_tag="VF240_18360"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7177226.1"
                     /translation="MTRFLRIAPALLALLVFAALPAAAQTTTTATLSGVVQDEKEAAI
                     PNATVTVRNVETNDSRSAQTDSDGRYRVVNLPVGPYEVTVESSGFSKHVQTGITLLLN
                     QQAVVDVTLKAGRIEEVVTVTENAALLNTATAEVATRFDERRLSELPLATNRNVYTVA
                     LSAAGVSQLQSNQAAFTNGINYSSNGGRLRSNNFLIDGQDNNDFGVAGAAIGLNNPDL
                     IQEVRLVTNQFTAEFGRNSSSVFNAITKSGSNEFHGSGFWFHNDNSLNACSNLNKAAG
                     FCNPAATDPSRTKAPFRIENQLGGTIGGPLNLPRFGEGGPAWFSGKDQTFFFFSIQRW
                     WDRRLGQGVSVDGVPTEAGRAVLQQQAGNRPQVAALLNFLPAAQTSLGTTESFTLAGT
                     SFPVQLGRLTGSSSFVFNDWQTSFRVDHRFNDDHTFNARYIYQDQDTVGAGQSTPPGY
                     ESSNVSRNQGLNFALTSVLSPTVVNEARAAYLRAASATVAADLRAEAIPSIQITSLNL
                     QGLNAGATRTAIGLAANLPQNSFRNTYQLQDNLTWSKGNHGLKFGVDIRRQQLAQLFK
                     PTSRGHLVFNNLNNFVNDVGNVQINRDLPGVARVLHLDWHDFYFYGQDEWRIRPNFTL
                     TYGLRYENAGQPIQDLIEFNAPVLAASGNNPVFQVRPIPGRDKDNLQPRIGFNWNPRT
                     REGGPLGWLTGGDKLVLRGGYARTHDYAYTNIALNIWSSFPFVAAFNQTNVAGAFANY
                     QTLPLNPLSFTRTQVTEDFASPAYDSFSLEVQRELTRDLVLRVGYVGSKGTGLFESID
                     GNPRRVGCVPRAAFPAECNVDQTVGTIRLRANSGQSIYHSMQSSLEKRLSRGFSAGLH
                     FTWSAFIDTMSEIFNNSASEIALAQEPYNRRLDRGRSSYDRPLRLSGNFVYELPWFRD
                     QQGFVGHVLGGWQLNSFFTFQSGAPFSPLNGSDPTGALGGLASAIGIATRPNLYTNLD
                     VARMTVEELYRIDQQLRAQAIAQAQAIFATLPANQPAGALPGAALPLTLFAAPAGRVV
                     VAANGTRSVVVDFNGLAAGQRAGTAGRNILRADGIGNIDFGILKNTRIGEDVRLQLRA
                     DFFNLTNTRNFGTPASSVTGGTNFLNQWSTDGGNRRIIVGVRVVF"
     gene            7986..8597
                     /locus_tag="VF240_18365"
     CDS             7986..8597
                     /locus_tag="VF240_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177227.1"
                     /translation="MKVFVLPPLLLLLAAFAAPAPARAQDTPAAALEVVKHSWSKERL
                     NWERDPFSGVIENFDDARVRMRNESRVARTKGTPEGDRIDREARADQSIIAARHQSKP
                     ARYAFLYKASLRNTADRPTRTIDWDYVFTDAATGEEVGRHQFTSDEKIGPGKQGGVVV
                     YIAAPPARTISVNSLNSKERDSLNGRVEVVRILYADGSVWERK"
     regulatory      8735..8928
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9369..11888
                     /locus_tag="VF240_18370"
     CDS             9369..11888
                     /locus_tag="VF240_18370"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7177228.1"
                     /translation="MRRNHYGAGRAPAKITHALFILLACAGVCHARQQQQQPSSSSSA
                     AVVAGRVTDERGGALQGASVTLYERASTVATRLATATDGAGRYSFERLAPGEYVLEAE
                     AEGFAASRARVLRVARASSQLDIDITLEVAGVREEVVVTAQDAPQTSDEVSKAVDVVT
                     GREMDERNEYSIAEALRTVPGLRVQQSGGPGAFTSIKTRGLRNQDTAVLIDGLRLRDV
                     TAPQGDASGFLGDLVSTGAGRVEVLRGSGSSLYGTNAVGGVVNIVTEEAGGPFRGQLL
                     AEGGGLGLARGRLQLAGGAGEADRLVYSAALSHLNVSRGIDSEDAARNTSAQGRVVVR
                     LTPTTTFSGRLYAADSFAQLNENPLAVGALPASGVIDARPLAPSELRRFNGGTPLGDL
                     NLGDATFIPGADDADNSRAGRFLSGALTFAGRPSENVGYSVTYHGLATRNSFREGPSV
                     SGGVFDFLFFDPTGSTRNDFDGATHTLNVRADFRLSRHNAVTAGYEFERENFLNDTLA
                     VLPSENSTVEVSERSHAFFVRDQLRFLDDRLQITAAFRAQFFSLSTPQFTPAAGSPFG
                     DIQFVAPADAYTGDGSVAYFFQRTNTKLRAHVGSGYRKPSLFERFGSFLCTFCGGAPA
                     FLPLGDPRLAPERSIAFDAGLDQTLARDRVRLSATYFYTRLQEVVGFGPVGASDPFGR
                     TFDGYVNTGGGLARGLELSARLAPARALDLFVSYTHTNSDQRRPVVAGVLEAFGIPDH
                     QFSLVATGRVGRRVLLNFDLAATSDYLAPLFDPATFSSRAFRFRGLVKGDLGASYTWP
                     LSETRSLRFFGHVENLFDRDYYENGFRTPGRHGRAGAALNF"
     gene            12274..13233
                     /locus_tag="VF240_18375"
     CDS             12274..13233
                     /locus_tag="VF240_18375"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7177229.1"
                     /translation="MNKNMSSCRSPLESLRVVRALMFAALLCLGAVSCGREGGIGSSA
                     GGVSEPAVGAARPRRIISLSPSATEILHGVGAFERVVAVSSYCDFPPQVATLPRVGGW
                     NNPNLEQIASLRPDLVVFADAQEPFIKEKVEALGIKTLSIPSRSVEDALASIELVGGA
                     TGDAEAGRRLAAETRAKLDDVRARTAALPRRRVLCVVDRIPGTLRDIYAASEGSFPAQ
                     LVEMAGGSSVAPPSAGGWGKMQKEAIVALDPEIIIDLMMQSSEGGLSENTQEVWSELG
                     QIKAVREGRVHHLRDTKLIHPSQFVGDAALRFAELIHPEAFGK"
     gene            13355..14158
                     /locus_tag="VF240_18380"
     CDS             13355..14158
                     /locus_tag="VF240_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177230.1"
                     /translation="MTSHTQSSSTLIETRGLRYAYAPGMPEVVAGVSLVVPRGRLSAL
                     IGANGSGKSTLVRLLGGLFAPRAGEVLFDGAPLAALDARARARRIAYVPQTIATVFPF
                     TALEVVLTGRSPHTTRFRFEGARDLGIARDALAAVDATHLAARPVTELSGGERQLVTL
                     ARALAQEPECLLLDEPSASLDLKHRAGLVRHLRRLRDERGMTALVVTHDLQLLDPAFD
                     HVFAMREGQLVAAGAPAEVLRDEVLTEVFGDEHVRARRAFGRTFVWSEL"
     gene            14195..15241
                     /locus_tag="VF240_18385"
     CDS             14195..15241
                     /locus_tag="VF240_18385"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7177231.1"
                     /translation="MLVDETNETESALTEQSAIAGVRLRRALLILSAALVASTLFALW
                     AGYKRLDVAALWTDESARVVFFQLRLPRVLMAGVVGASLGVVGAALQALFRNPLAEPL
                     TLGVSGGGALGASVAIAFGLGARVAGLPFVFVAAFLGALASVLVVYRLARTGAVVLPG
                     ALLLAGVVMNTVALSCVVVVQYFADYSRALQILRWTIGSLDTVGFDLLWRMLIFLVPG
                     WIALFVMARDLHLLAVDEETAASLGVNVRRSERLVYAAAALVVGVTVAVGGTIGFVGL
                     IVPHAVRLLFGEDVRVVLPCSFVLGAAFLMLADAAARTVLVAGELPVGAITAVAGGPV
                     FLWLLRRQQRYAAM"
     gene            15348..15926
                     /locus_tag="VF240_18390"
     CDS             15348..15926
                     /locus_tag="VF240_18390"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7177232.1"
                     /translation="MSIATKHGDKGETGLIGGERVSKADARVEAYGTIDELVSAMGLA
                     RSICDDAELRELTKAIQRELFTVAGAVANPASGEAEPTTYVTPEMVEALTAHVNRIEA
                     VEGILSDWSLPGEHAAAAAYDVARTVCRRAERCVVRLAEGGEAVNAQVVPYLNRLSDL
                     LWLIGRLLELRAGADARLRDDEHTGPRWSRAW"
     gene            complement(16041..16448)
                     /locus_tag="VF240_18395"
     CDS             complement(16041..16448)
                     /locus_tag="VF240_18395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7177233.1"
                     /translation="MEIIKVRSHEREELFEFTDEVRRRLTASGARAGLCVLYVQHTTA
                     GLTVNENADPDVPRDMLHCLRTLIPQHGMGFRHGEENSDAHIKATLVGSSVTIPFHDG
                     RLLLGRWQGIFLCEFDGGRERNVIMQVMRAVSD"
     gene            complement(16524..16940)
                     /locus_tag="VF240_18400"
     CDS             complement(16524..16940)
                     /locus_tag="VF240_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177234.1"
                     /translation="MCLFLLLTVFLSDAAAQGEVSAPRSGDRERRAILDALRVPVEKE
                     LGPKVVFKVDHLKVQGGWAFLRGVPQRPDGKAVDYKGTVYEQAREDGVFDDWVCALLR
                     KRGGKWQVVKYVVGATDVPYVGWDEEFGAPAAIFKD"
     gene            complement(17084..18814)
                     /locus_tag="VF240_18405"
     CDS             complement(17084..18814)
                     /locus_tag="VF240_18405"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177235.1"
                     /translation="MMKPTIPTDEAARLDALRRYEILDTAAEESFDELTRLAAQICGA
                     PVALISLVDAERQWFKSRTGLDAAETPRDVAFCAHAILSPDLFVVADASADERFAANP
                     LVTADPRIRFYAGAPLTTADGHNLGTLCVIDRVPRELDEGQRRALQTLARQAMLQIEL
                     RRTAAELARLNRALDRESTERRRVAAEIDFQAHLLDMVEQAVIATDLNGLIIYWNKYA
                     ESLYGWTAAEVVGRNILEVTPAAATEQQVAEIMSNLKEGKSWSGEFALRRRDGTTFPA
                     QVTDSPIYDAERRLIGIVGVSADISERMRAEGERAELLRREQAARAEAERANRAKDDF
                     LAIISHELRTPLTSMMGYVEMLRLDMLDERGRSRALEVIERNSQALAQLVGDVLDTSR
                     VVSGKMHLDTRPLDLHEVIGAAVDEVRASLEAKALTLHTSLDPNEGLMTGDPNRLRQV
                     VANLLSNAVKFTPRGGEVSVTLRRVGLEVEVAVSDTGAGIAPDFLPHVFERFRQAEGA
                     HQRGHSGLGLGLAIVRHIVELHGGAAAAESDGPGHGATFRVRLPLTDARAEAPQQTTH
                     AAGAGSTPTP"
     gene            complement(18939..20933)
                     /locus_tag="VF240_18410"
     CDS             complement(18939..20933)
                     /locus_tag="VF240_18410"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX7177236.1"
                     /translation="MNGQSIKLFFAGLVLLAALPATAAAKDTWTSVRSKNFFLIGNTS
                     EKEIRQVATRLEQFRHVFSRLFKRARLSDAVPTTVVVFKDAGSYKPFNPGGHAGYFQS
                     GEEVNYITLNAKLGPDMENPFGIIFHEYMHLLVKNNVAANTPAWVNEGLAEFYSTLEV
                     TKGGRAAEVGRPIAPHVFYLRQQKLLPLATLFAVDHSSPHYNEKSKRGVFYAQSWALV
                     HYLMFGKERQRQPQLNRYMDLARAGVSPEESFKQAFAADAPAIEKELRDYIRRDEYTF
                     VTFPFEERLEFDAGMTAAPLTEGQALGYLGDLALHTRLPELAETHLQKALALEADNAM
                     AHASLGVLRARQRRFAEAKEHLGRAVAADARNHLAHYYYAHTLSREGMDETGLVGGYT
                     EQAANQMRAALAKAIKLKPDFAEAYHLAAFVNLVTGEQIDESIELLKRAIEIAPGREE
                     FTLSLAQLHLRKQDFAAARKLIEPLAQSSTDAQLRANAQSLLNNIVNFEGRASRLQPD
                     SAGASAHDDDNASPASGEGPPRLLRRGASAGAQETGKTPDELVADALSEAVLEALRKP
                     LTGEARAQGLLARLECDAKGIVFHVRVGGRVLKLRSAGFDGLHVMAFTQEAGGQISCG
                     ARKPESLVVVTYRAASDARAKSDGEMVALEFVPAAFVLKQ"
     gene            complement(21139..21741)
                     /locus_tag="VF240_18415"
     CDS             complement(21139..21741)
                     /locus_tag="VF240_18415"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX7177237.1"
                     /translation="MSTSYAKAETLVGAFVAPTDWLKSAALVVAFSLLTALAAQVVIP
                     LPWTPVPLTGQTFAVLLTGALLGSRLGALAMLAYLAEGAMGLPFFRAGAGGVGHLLGP
                     TAGYLLAFPAAAFVTGLLAERGWDRRFLTAAAAMLLGSFVILAGGWAWLAFALGSAAR
                     AFQLGVAPFLIGDVVKITLAAALLPAGWALLGRRRGGQRK"
     gene            complement(21880..>24277)
                     /locus_tag="VF240_18420"
     CDS             complement(21880..>24277)
                     /locus_tag="VF240_18420"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX7177238.1"
                     /translation="CFAGAVFGLVMFFRLRSMAVHHSMRTISELIYETCKTYLITQGK
                     FILILEAFIALVIILYFGVLLDFEAARVAIILFFSLVGIAGSYGVAWFGIRVNTFANS
                     RTAFASLGGKPYPLYAIPLKAGMSIGMMLISVELLIMLCILLFVPRDYAGPCFIGFAI
                     GESLGAAALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCTGDNAGDSVGP
                     SADGFETYGVTGVALITFILLAVPSATVQVQLLVWIFVMRVMMIVASALSYFVNEAIA
                     KSRYEHAQTMNFEKPLTSLVWITSIVSVVLTYVVSYLMIPTLGGDDSLWWKLSTIITC
                     GTLAGAVIPELVKVFTSTESRHVKEVVTSSGAGGASLNILSGLVAGNFSAYWIGLAIT
                     ALMGIGYIVSTQGVGALMAAPAVFAFGLIAFGFLGMGPVTIAVDSYGPVTDNAQSVYE
                     LSLIEQVPGVREEIARDNKFEVNFERAKHLLEENDGAGNTFKATAKPVLIGTAVVGAT
                     TMIFSIIQVLRGNAVAGASAAGLSAERGLANFYENLSLLNPLFLLGLITGGAIIYWFT
                     GASTQAVTTGAYRAVQFIKANIRLDETVVKASVEDSKKVVQICTEYAQKGMFNIFLTV
                     FFSSLAFAFIDPYFFIGYLISIALFGLYQAIFMANAGGAWDNAKKIVEVELKQKGTPL
                     HDACVVGDTVGDPFKDTSSVAMNPVIKFTTLFGLLAVELALSVYRSRGGDASLNYMLA
                     AIFFLISVYFVWRSFYGMRIGTEIEAAHPGETPIGELAAGEMGTDTEGVRQQSADRVR
                     "
BASE COUNT         4057 a         8356 c         7982 g         3882 t
ORIGIN      
        1 gatgaaactc tctcgacgca ctactgtcgc cagcgccctc tgctacagcg cgctcgcgct
       61 cgccgccgcg gcgggctgta actccacgcc cgaagccaac agagcagcga ccaacgccgc
      121 gaacgccgcc gccacgcctt cgccaaccgc gcagacgctc tccgcgaccg agcgcccgca
      181 gaagatcaaa gaccagatgg cgcagcgcgg cgaacaggat caggccagcc ccgtcctgaa
      241 gatcgccgag ccggccgagg gcgcgaccgt ctccggcgcg acggtgcgcg tccggctcaa
      301 cctgagcggc gacctcaagg gctaccagcc gcacaaagac ccggcgacgc agatgggcaa
      361 ccacatccac gtcatcctcg acaacgagcc ttacgaggct tactacgaac tcggccgccc
      421 cttcgagctg cgcaacgtcg ccgccggtaa gcacaccttg cgcgtcttcg cctcgcgccc
      481 gtggcacgag agctacaaga acgacggctc gttccagatg gtcaccttca acgtgagcgg
      541 cggcggcgac actacccagc cgaccaccgc cagcaacgga cagcgcatgg ccgacaaccg
      601 cgcgccggcc gccaacacca atgccgcggc cacgcccgcc gcctccccct ccgccgaggg
      661 caaagacttc ggcgcgaagc ccaacgcggc cggcgacgtt gaccacacga agccgctcct
      721 gacctacagc cgcccgaagg gcgagtacaa ggacgccgag gccgaggcca tcatgatcga
      781 cttctggctg gcgcacgcca agctcgaagg cgacggcggc gagtaccgcg tgcgctactc
      841 cgtggacggc gccccgccgc agcacctccg caagtgggaa cccatctggc tcaagggctg
      901 gacggccggc aagcacaagg tcaaagtcga actcgttgac cgcaacgaca ccgtcgtcca
      961 gaacggcggc tacaactcga ccgagcggga gatcacggtc acgaagtagt gaggcgatgt
     1021 tcacttgtgt gaaagcgaca tagattcacc gcagagacgc agagggcaca gagttcgcgc
     1081 agagggttga ctctactcaa gcctgcggcg cctctgcggt gagtctgtct tactacccac
     1141 tcaaccgatt tgtcagaagc cttgacgaac atgaacgaag cggccgagac cgagcgcgac
     1201 gcacacgccc tccccgcgca ctacggcgac gcgcgcgccg agtacgacgc cgttcgcggc
     1261 cggcgcgcgg gactcatcga cctctccgcg gcgcgcggcc gcgtcgaggt gtcgggcgcg
     1321 gaggccgtgc agtttttgaa cggcatggtg acgaacgacg tgaaggcgct cgaagacggc
     1381 gcgtggatgc acgccgcctt cccgaacgtg cagggccggc tcctcgccag cgtccgcgtc
     1441 atccgtcgcg gcggcgcata cctcttcgac accgaggcgg cgacgcacgc cgtagtcctc
     1501 aaaaacctcg aacgtttcac gctcgccggc gacttccgcg tccgcgacgc gagcgccggc
     1561 acagggatga tctccgtgca gggcgcgggg tcgcgcgagg tcgtccgcgc cgtcgcgggc
     1621 gaggaggccg cggggctgcg tcgaggccgc gcggctgagt gggagtgggg cggcgcacag
     1681 actctcgtcc tgcgtgcgac gcacacggcc gaggacggct tcgacttcgt gtgcgacgcc
     1741 ggattggccg tcaacctgtg ggagtcgtta gcgagcgcgg gcgcgcggcc cgtggggttc
     1801 gacgcgctcg aagttctcag aatcgaggcc ggcatcccgc gctacggcgt ggacgtggac
     1861 gacacgaacg tggtgctcga agccgcgttg gatgaagccg tgagcttcac gaagggctgc
     1921 tacgtcgggc aggaaatcat cgcgcgcatc cactggcgcg gccacgtcgc caagcgtctt
     1981 gccgggcttg tcctcgaaga cggagacgcg cccgcagcgg ccggcgcgaa ggtcttctca
     2041 agcgacgggg cgagggaaat cggccgcgtc acttcgtccg tttactcgcc acggctgggc
     2101 cgcgcggtcg cgctcggcgt cgttaagtac gacttcctca gcgcgggtac ggaggtgcgc
     2161 gttcgtgacg gcgacggcgg cgcgggaagc gagcgcgcgg cgcgcgtggc ggagttgccg
     2221 ctcgtgcgcg gaagttggta cgactcggcc ggcgacggcg cggaggacgc atgagcgaag
     2281 agacgccgcg cgagaattcc gcttcgacgc cctccgcgca gaatctcgct tacgagcagg
     2341 cgcgcctcgc ctactccatc atccagacgc tcctcgaaca cacgcgcgtc acgggcgacc
     2401 tcgtcgcgct catggcgcgc gccatcgacg aggacacgct caaggccctc accggcacac
     2461 cccactgggc cgcctacctc gacagccgcc gcgcgctgga gcgcacgcgc gcggacgtgg
     2521 aaaaattcgc cgccgagtgg acgcgcatgt ccgaagagac ccctgccccg cccccatcca
     2581 ttcaaaaaag cgaaggctga tcctccagct ctccggggtc aacgaccgct tgtccgagca
     2641 gccgcttgag gcggttggcg gaggcgggcg cgtggccctc gtagaagttg ctgaagtagg
     2701 cgaagacgga ggggacgcgc tcgcgcagac gttcgacggc ctcggcccat cgttcgaggt
     2761 tggcgccgcg gtcgcgctgc accacgtcga agcgcgtgat gtcgcgttcg cccatccagc
     2821 ggacgtagac gaagtccgtc acttcgagca cggggacggc cgcgcgccag atgcgctcgc
     2881 gcgtcgccca cggcccctcg acgagggcga gcgagacgtt gggacggccg ccgtacacga
     2941 gcggcgcgac gaactcctcc tcgaaccaga acgggtcgcg gaactcaatg gcgaagcgca
     3001 cgtccggcgg cagcagcggc aggaaggcgg agagcgccgc gaagttctcg cgcgtcgcct
     3061 cgaactgcgg cgggagttgc acgagcacgg cggccaactt ctcccgcagg ccgcgcgccc
     3121 tctcgcaaaa agcttcgagt agcgcgcggg cctcgccgcc gcgcagggcc aggcggtgcg
     3181 tgatctcctg cggcagcttg agcgaaaagc tgaagccttc aggcgtcctc ttgcgccagc
     3241 cttcgacggc cgactctgtg gggatcgcgt agaaggtcga atcgacctcg acggtgtcga
     3301 aagagcgcgc atactcttcg agcatccggt cggcgcgcgt cccccgccgg tagaagacgg
     3361 ggcgcgcgct cgccggcggc gtcacccagt cgtcatagtt ccagccctga cagccgacgt
     3421 gtacgccgcg gaagttctct gttgaagaca tgatttagcg ttcaaacgcg acagacgatg
     3481 atttgaaaaa cgccgcgcca gtgtggctcg acgcgctcga cccagaggcc cgcgcggcgc
     3541 gcctcttcgg cggtgcgccg gttgaagtgg tcgtcgcaga gcgcgaccgt caacttgctg
     3601 aaggcgtcga aaacgtaacc gagcaacccc tccggccgca cgtgttcgag gagcacgacg
     3661 cgtccaccgg ggcgcaccac ccgtcgcaac tcactgaagg cgcgcgccgg cgaccccacc
     3721 gagcagaaga cgagcgtggc gaggccggcg tcgaacacgt cgtcggcgaa cggcaactcc
     3781 tcggcccgcc cgcgcacgag ccacagcccc ggcgggcgct ttgccctcac ccgcgcccgc
     3841 tccaacatct cgcggctcgg ctccacgcac gcgccccgcg cgccctccgt gtagaactga
     3901 aagttcgccc ccgtccccac gccgacctca agaacgcgcg cccgctccgg cagctcacgc
     3961 agggccgacg cgcgcaggcg cccgagcagc caacgctcca gcggccgcat cgcgccgtcg
     4021 tagtcagcgg ccagacggtc gtagacgtgt gagccttgtg ccatcaacag aggcgtgccc
     4081 ccacggcccc gcgcgccggc gcgcgactgc cgttgtatat aaaatcgtat ggtttctacg
     4141 aaaaattgga taaatatcca tcaaccgtca aattatatac agcaaaatgt gcgagttagg
     4201 atgcgccccc caacgggcat actgcttgcg tcgctcgttc ccatcagcag acgccctgaa
     4261 gcgtcatccc agggcttatc ttctcattta gataacatct gccttcgcca aggcccggaa
     4321 ttaagctcgg cggtcagttg aaagcggcct ccccgaaacg gccgggggcc gacttcaccc
     4381 aaccatttca gaacctgact tcttcggagg aaactcttca tgacgagatt tctgagaatc
     4441 gcccccgctc tcctcgccct gctcgtcttc gcggccctgc cggccgcggc ccaaacgacg
     4501 accaccgcca ccctctccgg cgtcgtgcag gacgagaagg aagcggccat ccccaacgcg
     4561 accgtcaccg tgcgcaacgt cgagaccaac gactcgcgct cggcgcagac cgactccgac
     4621 gggcgctacc gcgtcgtcaa cctccccgtc ggcccctacg aggtgacggt cgagtcctcc
     4681 ggcttctcga agcacgtgca gaccggcatc acgctgctgc tcaaccagca ggccgtcgtt
     4741 gacgtgacgc tcaaggccgg ccgcatcgag gaagtcgtca cggtgacgga gaacgccgcg
     4801 ctcctcaaca ccgcgaccgc cgaggtcgcc acccgcttcg acgagcggcg cctctcggaa
     4861 ctgccgctcg ccaccaaccg caacgtctac accgtcgcgc tgtcggccgc cggcgtcagc
     4921 cagctccagt cgaatcaggc cgccttcacc aacggcatca actactcgtc gaacggcggc
     4981 cgtctccggt cgaacaactt cctcatcgac ggtcaggaca acaacgactt cggcgtcgcc
     5041 ggcgcggcca tcggcctcaa caaccccgac ctcattcagg aggtgcgcct cgtcaccaac
     5101 cagttcacgg ccgagttcgg ccgcaactcc tcctcggtct tcaacgccat caccaagtcg
     5161 ggcagcaacg agttccacgg ctccggcttc tggttccaca acgacaactc gctgaacgcg
     5221 tgcagcaacc tcaacaaggc cgccggcttc tgcaacccgg cggcgaccga cccgtcgcgc
     5281 accaaggccc cgttccgcat cgagaaccag ttaggcggca ccatcggcgg cccgctcaac
     5341 ctgccccgct tcggcgaggg cggccccgcc tggttctccg gcaaggacca gaccttcttc
     5401 ttcttctcga ttcagcgctg gtgggaccgc cgtctcggcc agggcgtgag cgttgacggc
     5461 gtgccgaccg aggccggccg cgccgtcctc cagcagcagg ccggcaaccg cccgcaggtg
     5521 gcggcgctcc tcaacttcct gccggcggcg cagacctcgc tgggcaccac cgagtcgttc
     5581 accctcgccg gcacttcgtt ccccgtccag ctcggacgcc tgaccgggtc gagcagcttc
     5641 gtcttcaacg actggcagac ctcgttccgc gtcgatcacc gcttcaacga cgaccacacc
     5701 ttcaacgcgc gctacatcta tcaggatcag gacacggtcg gggccggcca gtccaccccg
     5761 ccgggctacg agtcgtccaa cgtctcgcgc aaccagggcc tgaacttcgc gctgacgagc
     5821 gtgctcagcc cgacggtcgt caacgaggcg cgcgccgctt acctgcgcgc ggccagcgcg
     5881 accgtcgccg ccgacctgcg ggccgaggcc atcccctcca tccagatcac ctcgctcaac
     5941 cttcagggcc tcaacgcggg cgcgacgcgc acggccatcg gcctcgccgc caacctcccg
     6001 cagaactcgt tccgcaacac ctaccagctt caggacaacc tcacctggtc gaaggggaac
     6061 cacggcctca agttcggcgt tgacatccgc cgccagcagc tcgcgcagct cttcaagccg
     6121 acctcgcgcg gccacctcgt cttcaacaac ctcaacaact tcgtcaacga tgtcggcaac
     6181 gtccagatca accgcgacct gccgggcgtc gcccgcgtgt tgcacctcga ctggcacgac
     6241 ttctacttct acggtcagga cgagtggagg atccgtccga acttcacgct gacctacggc
     6301 ctgcgctacg agaacgccgg ccagcccatc caggacctca tcgagttcaa cgcgccggtg
     6361 ctcgccgcct ccggcaacaa ccccgtcttc caggtgcgcc cgatcccggg ccgcgacaaa
     6421 gacaacctcc agccgcgcat cggcttcaac tggaacccgc gcacccgcga gggcggcccc
     6481 ctcggctggc tgacgggcgg cgataagctg gtcctgcgcg gcggctacgc ccgcacacac
     6541 gactacgcct acaccaacat cgcgctcaac atctggagct cgttcccgtt cgtcgccgcc
     6601 ttcaaccaga cgaacgtcgc cggcgccttc gccaactacc agacgctccc gctcaacccg
     6661 ctctccttca cccgcacgca ggtcaccgag gacttcgcct ccccggccta cgactcgttc
     6721 agcctcgaag tccagcgcga gctgacgcgc gacctcgtcc tgagggtcgg ctacgtcggg
     6781 tcgaagggca cgggcctctt cgagagcatc gacggcaacc cgcgccgggt ggggtgcgtc
     6841 ccgcgcgcgg ccttccccgc cgagtgcaac gtcgatcaga cggtcggcac catccgcctg
     6901 cgcgccaact ccggccagtc catctaccac tcgatgcagt cgagccttga gaaacgcctc
     6961 tcgcgcggct tctcggccgg cctccacttc acgtggagcg ccttcatcga cacgatgtcg
     7021 gagatcttca acaactccgc ctccgagatc gcgctcgcgc aggaacccta caaccgccgg
     7081 ctcgaccgcg gccgctcctc ctacgaccgc ccgctccggc tctccggcaa cttcgtctac
     7141 gagctgccct ggttccggga tcagcagggc ttcgtcggcc acgtcctcgg cggctggcag
     7201 ctcaactcgt tcttcacctt ccagtcgggc gcgcccttct cgccgctcaa cgggtcagac
     7261 ccgaccggcg cgctcggcgg cctcgcctcg gccatcggca tcgccacccg gccgaacctc
     7321 tacaccaacc tcgacgttgc gcgcatgacg gtcgaggagc tttaccgcat cgaccagcaa
     7381 ctgcgggcgc aggccatcgc gcaggcgcag gccatcttcg ccacgctgcc ggccaaccag
     7441 ccggccggcg cgctccccgg agcggcgttg ccgctcacgc tgttcgccgc gccggcgggg
     7501 cgcgtcgtcg tcgccgccaa cggcacgcgc agcgtcgtcg tcgatttcaa cgggctggcg
     7561 gcgggccagc gcgcgggcac ggccggccgc aacatcctgc gcgccgacgg catcggcaac
     7621 atcgacttcg gcatcctgaa gaacacgcgc atcggcgagg acgtgcgcct ccaactgcgg
     7681 gccgacttct tcaacctgac gaatacgcgc aacttcggca cgccggcctc gtccgtcacc
     7741 ggcggcacga acttcctcaa ccagtggagc accgacggcg gcaaccgccg catcatcgtc
     7801 ggcgtccgcg tggtcttctg aggcggccga gccggcgcgc ctcagacaca actcggacgg
     7861 gccgggggac ggacgcttcc cccggccctg ttttttttcg cgctttttcg cgcgccgcga
     7921 cctgtgcgat actccacgca aacgaccaca cgcagagaga gagagctgca acggagggcc
     7981 gagccatgaa agttttcgtc ttgccgcccc tactcctcct tctcgccgcc ttcgcggcgc
     8041 ccgcgccggc ccgcgcacag gacacgcccg cggcggcgct cgaagtcgtc aagcatagct
     8101 ggagcaagga gcggctcaat tgggagcgcg accccttctc cggcgtcatc gagaacttcg
     8161 acgacgcgcg cgtccgcatg cgcaacgaga gccgcgtggc ccgcaccaag ggcacgcccg
     8221 agggcgaccg catcgaccgc gaggcgcggg ccgaccagtc gatcatcgcc gcccggcacc
     8281 agagcaagcc ggcgcgctac gcctttctct acaaggcctc gctgcgcaac acggccgacc
     8341 gcccgaccag gaccatcgac tgggactacg tcttcaccga cgccgcgacc ggcgaggagg
     8401 tgggccgtca ccagttcacc agcgacgaga agatcggccc cggcaagcag ggcggcgtcg
     8461 tcgtctacat cgccgcgccc ccggcccgca ccatcagcgt caactccctc aactcgaaag
     8521 agcgcgactc cctcaacggc cgcgtcgagg tcgtccgcat cctctacgcc gacggctccg
     8581 tctgggagcg gaagtaaagg cgctcggcgg cgcgggtcgg cttgacgcgg cgcgagtcgc
     8641 ggtgttaggt tgctggccgc cgcggcgaaa accgccgcgc atgttctttg gacaattcgt
     8701 cacatcgcta aatcaactcc cgcgctaacg gcgcgagggg ttgaccgtcg caggttgaca
     8761 agggaagtcg ggtgcaaagc ccgcacggtt cccgccactg tgtaccggcg attgtgccgc
     8821 ccgcgaatca tgccactggc gcttcttcaa catacagcgc cgggaaggcc gcggccggcg
     8881 cgtccgagcc gggagtcagg aaaccttgcc tgcaagatta gaggtcagag gtcagaggtt
     8941 agaggtcagt aaagccaagc tgtttttact gacctctaac ctctgacctc tgacctctgc
     9001 tttggacgga ggcgaccgct tgtgccccgg gaaagggcga gtggcgtcca ggcgttgatc
     9061 ttgagaaggc cgcccccgcg tggcgcgcgc gaagtttccc gacgcgacgc gcggcggcga
     9121 cagccttcga ttcaaagttc aaccgcatga ggccctcggt cttccccact tcagggaaga
     9181 ccgagggcct tttttgtggt cggacgtggc ggcgcatttc ggtcggatga ccccgacgcg
     9241 ccgcccaaga tacacacgca ttttccgtcc ttctcaagag ccgctggcac acggccgttc
     9301 agcactgagt ctttaatttt ggctgatcgc tgaaagctaa aagctaacgg ctttaaggaa
     9361 ttcaaacgat gagaaggaat cactacgggg ccggccgcgc gccggcgaaa atcacacacg
     9421 ccctcttcat ccttctggcg tgcgcgggcg tgtgccacgc gcggcagcag cagcagcaac
     9481 cgtcgtcgtc ctcttctgcc gccgtcgtgg ccggtcgtgt cacagacgag cgcggcggcg
     9541 cgcttcaggg cgcgagcgtg acgctctacg aacgcgcctc gacggtcgcg acgcgcttgg
     9601 ccaccgcgac cgacggcgct ggccgctaca gcttcgagcg tctcgcgccc ggcgagtacg
     9661 tgctcgaagc cgaggccgag gggttcgccg cgtcgcgcgc gcgcgtgctc agggtcgcgc
     9721 gcgcgtcgtc gcagctcgac atcgacatca cgctcgaagt ggcgggcgtc cgcgaggaag
     9781 tcgtcgtcac ggcgcaggac gcgccgcaga cctcggacga agtctcgaag gccgtggacg
     9841 tggtgaccgg gcgcgagatg gacgagcgca acgagtactc catcgccgag gcgctgcgga
     9901 cggtgccggg cctgcgcgtc caacagtcgg gaggccccgg cgccttcacc tcgatcaaga
     9961 cgcgcggctt gcgtaatcag gacacggccg tcctcatcga cggcctgcgc ctgcgcgacg
    10021 tgacggcccc gcagggcgac gcctccggct ttctcggcga cctcgtctcg acgggcgcgg
    10081 ggcgcgtcga agtcctgcgc ggctccggct cgtcgctcta cgggacgaac gccgtcggcg
    10141 gcgtcgtcaa catcgtcacc gaagaagcgg gcggcccctt ccgcggccaa ctgctcgccg
    10201 agggcggcgg cctcggcctc gcgcgcggcc gcttgcagct cgcgggcggc gcgggcgagg
    10261 ccgaccgcct cgtctacagc gccgccctgt cgcacctcaa cgtctcgcgc ggaatcgact
    10321 ccgaggacgc ggcgcgcaac acgagcgcgc agggccgcgt cgtcgtgcgt ctgacgccga
    10381 ccaccacgtt ctcggggcgt ctctacgccg cggactcgtt cgcgcaacta aacgagaacc
    10441 cgctggccgt cggcgcgctg ccggcgtccg gcgtgatcga cgcccggccg ctcgcgccct
    10501 ccgagctgcg acgcttcaac ggggggacgc cgctcggcga cctcaacctc ggcgatgcga
    10561 ccttcatccc cggcgccgac gacgccgaca actcacgcgc gggccgcttc ctttcaggcg
    10621 cactcacctt cgccgggcgt ccgtcggaaa acgtcggcta ctccgtcacc taccacggcc
    10681 tcgccacgcg caactccttc cgcgaggggc cgtccgtttc cggcggcgtc ttcgacttcc
    10741 tcttcttcga ccccaccggc tcgacacgca acgacttcga cggcgccacg cacactctga
    10801 atgtacgcgc cgacttccgc ctcagccggc acaacgccgt caccgccggc tacgagttcg
    10861 agcgcgagaa ttttctgaac gacacgctcg ctgtcctgcc ttcggaaaac tccacggtcg
    10921 aagtctccga gcgcagccac gccttcttcg tccgcgatca gctccgcttt ctcgacgacc
    10981 gactccaaat caccgccgcc ttccgcgcgc agttcttctc actgagcacg ccgcaattca
    11041 cgcccgccgc cgggagtccc ttcggcgaca tccagttcgt cgccccggcc gacgcctaca
    11101 cgggcgacgg ctccgtcgcc tatttctttc aacggacaaa cacaaagctg cgcgcgcacg
    11161 tcggcagcgg ctaccgtaag ccgtcgctct tcgagcgctt cggcagtttc ctctgcacct
    11221 tctgcggcgg agcgcccgcg tttctcccgc tcggcgaccc gcgcctcgcc cccgagcgct
    11281 ccatcgcctt cgatgccgga ctcgatcaga cgctcgcacg cgaccgcgtg cgtctgtcgg
    11341 ccacgtactt ctacacgcga cttcaagagg tcgtcggttt cggccccgtc ggcgcaagcg
    11401 accccttcgg ccgcacgttc gacggctacg tcaacacggg cggcggcctc gctcgcgggc
    11461 tggagttgag cgcgcgcctc gcccccgcgc gcgcactcga cctcttcgtc tcttacacac
    11521 acaccaacag cgaccagcgc cgccccgtgg tcgcgggcgt gctcgaagcc ttcggcattc
    11581 ccgaccacca gttctcgctc gtcgccacgg ggcgcgtcgg ccgccgcgtc ctcctgaact
    11641 tcgacctcgc ggcgacgagc gactatctcg cgccactgtt cgacccggcg acgttcagtt
    11701 cgcgcgcctt ccgctttcgc ggcctcgtca aaggcgacct cggcgcgagc tacacctggc
    11761 cgctctccga gacgcgctcg ttgcgcttct tcggccacgt cgagaacctg tttgatcgcg
    11821 actactacga gaacggcttc cgcacgccgg gtcgccacgg ccgcgccggc gcggcgctca
    11881 acttttagcg agggatgagg gcggagggat gagggatgaa ggatgcggcg agcacagtac
    11941 cgcgagcggt ggcgagcggg tatgtctatg atgagaggcc agccgcgcgc acgcgagacc
    12001 cgcgaggctt ccgctcgcgg tactgatgtc gatgggtcaa tgagtcaatg agtcaatgaa
    12061 tcaatctcct actcatccct catccctcat ccctcatccc ttgaaagagg gtgaggatta
    12121 ttaccgtgaa ggtgcgttcg tggtcttcac cgcgcgctat cacttgcggc gcggctactg
    12181 ctgcgagagc ggctgtcgcc actgtccgta tcgaggcgtc gtcgtgccgg ccggcgcgag
    12241 tgaggacgct ttgactgatg aggcggaaag tcgatgaata agaatatgag ttcgtgccgg
    12301 tcgccactgg agagcttgcg cgtcgtgcgg gcgctcatgt tcgccgcgct attgtgtctc
    12361 ggggcagtct cctgcgggcg cgagggcggc atcgggagtt cggcgggcgg cgtctccgag
    12421 ccggcggttg gcgcggcgcg gccccgccgt atcatctcgc tcagtccgag cgcgaccgag
    12481 attcttcacg gcgtcggggc cttcgagcgc gtcgtggcgg tgtcgagcta ctgcgacttc
    12541 ccgccgcagg tcgccacgct tccgcgcgtc ggcggctgga acaacccgaa cctcgaacag
    12601 atcgcctcgc tgcgccccga cctcgtcgtc ttcgccgacg cgcaggagcc gttcatcaag
    12661 gagaaggtgg aggcgctggg gataaaaaca ttgagcatcc cgagccgttc ggtcgaggac
    12721 gcgctggcgt cgatagagct ggtgggcgga gccacgggcg acgcggaggc gggccgccgg
    12781 ctcgccgccg agacgcgcgc aaagctcgac gacgtgcgcg cgcgcacggc cgcgctcccg
    12841 cgccgccgcg tcctgtgcgt ggttgaccgc atccccggca cgctgcgcga catctatgcg
    12901 gcgagcgagg gaagtttccc ggcgcagctt gtggagatgg cgggcggctc gtcggtcgcg
    12961 ccgccgtcgg cgggcgggtg gggcaagatg cagaaggagg ccatcgtcgc gctcgacccg
    13021 gagatcatca tcgatctgat gatgcagtcg tcggagggcg gactctccga gaacacgcag
    13081 gaggtctgga gcgagctggg acagatcaag gccgtgcgcg aggggcgcgt ccatcacctg
    13141 cgcgacacga agctgattca cccctcgcag ttcgtcggcg acgcggcgct gcggttcgcc
    13201 gagctgattc acccggaggc ttttgggaag tagtgaggaa gggaaggatg ggccacggat
    13261 aaaacggagc ggacggatta acacggattg aatccgtgtc gatctgtttc atccgtctta
    13321 tccgtggccc atcccgccgt cccatgaagt cgagatgacg agccacacac aaagcagcag
    13381 caccctgatc gagacgcgcg gactccgcta cgcctacgcg cccggcatgc ccgaggtggt
    13441 ggcgggcgtg tcgctcgtcg tcccgcgcgg gcgtctgtcg gcgctcatcg gcgcgaacgg
    13501 ctcgggcaag tccacgctcg tccggctgct cggcggactg ttcgctcccc gcgccggtga
    13561 agtcttgttc gacggcgcgc cgctcgccgc gttggacgcg cgcgcccgtg cccgccgcat
    13621 cgcctacgtg ccgcagacaa tcgccaccgt cttccccttc accgcgcttg aagtcgtgct
    13681 cacgggccgc agcccacaca ccacgcgctt ccgcttcgag ggcgcgcgcg acttgggaat
    13741 tgcccgcgac gcgctcgcgg ccgtggacgc gacgcacctc gcggcacgcc ccgtcacgga
    13801 gctttcgggc ggcgagcgcc agctcgtcac gctggctcgc gcgctcgcgc aggagccgga
    13861 gtgcctgctc ctcgacgagc cgtcggcctc gctcgacctc aagcaccgcg ccggcctcgt
    13921 ccggcacctg cgacgcctgc gcgacgaacg cgggatgacg gccctcgtcg tcacgcacga
    13981 cctgcaactg ctcgacccgg ccttcgacca cgtcttcgcc atgcgcgagg ggcaactcgt
    14041 cgccgccggc gcgcccgccg aggtgctgcg cgacgaagtg ttgaccgagg tcttcggcga
    14101 cgagcacgtc cgcgcccgcc gcgcgtttgg acgcacgttc gtctggtcgg aactctgacg
    14161 attgcgaatg tggttggcgg attttaaagt agagatgttg gttgatgaaa caaacgagac
    14221 ggagtcggcg ctgacggagc aatccgcaat cgccggcgtg cgtctgcgcc gtgcgctgct
    14281 gattttgtcg gccgcgctcg tcgcctccac actcttcgcg ctgtgggccg gctacaagcg
    14341 gctcgacgtg gcggcgctgt ggacggacga gtcggcgcgc gtcgtcttct tccaactgcg
    14401 tctgccgcgc gtgttgatgg cgggtgtcgt tggggcgtcg ctgggcgtgg tgggggcggc
    14461 gcttcaggcg ctctttcgta acccgctggc ggagcctttg acgctgggcg tctcgggcgg
    14521 cggcgcgctg ggggcgagcg tggccatcgc gttcgggctg ggcgcgcgcg tggcggggct
    14581 gccgttcgtc ttcgtcgccg ccttcctcgg cgcgctcgcc tccgtcctcg tcgtctaccg
    14641 gctggcgcgc acgggcgcgg tcgtgctgcc cggcgcgctc ctcctcgccg gcgtcgtgat
    14701 gaacacggtg gcgctctcct gcgtggtcgt cgtgcaatac ttcgccgact actcgcgcgc
    14761 gctgcaaatc ctgcgctgga caatcggcag cctcgatacc gtcggcttcg acctcctctg
    14821 gcgcatgctc atctttctcg ttcccggctg gatcgccctc ttcgtgatgg cgcgcgacct
    14881 gcacctgctg gcggtggacg aggagacggc ggcgagcctc ggcgtcaacg tgcggcgcag
    14941 cgagcggctg gtgtatgcgg ccgcggcgct cgtcgtcggc gtgacggtgg ccgtgggcgg
    15001 caccatcggc ttcgtcggcc tcatcgtgcc gcacgccgtg cgcttgctct tcggcgagga
    15061 cgtgcgcgtc gtgctgccgt gctcgttcgt cctcggcgcg gcgtttctga tgctggccga
    15121 cgcggcggcg cgcaccgtcc tcgtcgcggg cgagctgccc gtcggcgcga ttacggccgt
    15181 cgccggcggc cccgtcttcc tgtggctgct caggcgacag cagcgttacg cggcaatgtg
    15241 aagagcagcg gttagaggtc agaggctaga ggtcagtgag agacagattc actcattact
    15301 gacctctgac ctctgacctc tgacctctgc actccgaagg agtgcaaatg agcatcgcga
    15361 caaagcacgg cgacaagggt gagacgggac tgatcggcgg cgagcgcgtg tcgaaggccg
    15421 acgcgcgggt cgaagcctac gggacgattg acgagctggt gtcggcgatg ggcctcgccc
    15481 gttccatctg cgacgacgcc gagctgcgcg agctgacgaa ggcgattcaa cgcgagctat
    15541 tcacggtggc gggcgcggtc gccaaccctg cgagcgggga ggccgagccg accacttacg
    15601 tcacgccgga gatggtcgag gcgctgacgg cgcacgtcaa tcgcatcgag gcggtcgagg
    15661 ggatactctc ggactggtct ctgccgggcg agcacgcggc cgccgccgcc tacgacgtgg
    15721 cgcgcacggt ctgccgccgc gccgagcgct gcgtcgtgcg tctcgccgaa ggcggcgagg
    15781 cggtgaacgc gcaggtcgtc ccctacctta atcgcctctc cgacctgctg tggctcatcg
    15841 gccgcctgct cgaactgcgc gcgggcgcgg acgcgcgcct ccgcgacgac gaacacacgg
    15901 ggccgcgttg gtcgcgcgcg tggtgatgag ggagcggtta gctattagct cttagctttc
    15961 agcccttcgc ctcgccgtgg cttaacatga ttgatgggcc gccgcccgcc cgaaaagttt
    16021 tataagctaa tagctaataa ctaatcgcta actgctctca tcacttgcat tatcacgttg
    16081 cgttcgcgcc cgccgtcgaa ttcgcagagg aagatgccct gccagcggcc gaggaggagt
    16141 cggccgtcgt ggaaggggat ggtgacgctt gagccgacga gcgtggcctt gatgtgcgcg
    16201 tcggagttct cctcgccgtg gcggaagccc atgccgtgtt gggggatgag cgttcgcagg
    16261 cagtggagca tgtcgcgcgg aacgtccggg tcggcgttct cgttgaccgt cagccccgcc
    16321 gtggtgtgct gcacgtagag cacgcacagc ccggcgcgcg cgcccgaagc cgtcagcctc
    16381 cggcgcacct cgtccgtaaa ctcgaacagc tcctcacgct cgtgtgagcg aaccttaatg
    16441 atctccatga gagcgatcct cccagagcgc ccgccgcgtc tgtcggccgg cggcgcgcgc
    16501 gacgcatcta atccgccgcc ggcctaatcc ttgaagatcg cggccggggc gccgaactcc
    16561 tcgtcccaac ccacgtaggg cacgtcggtc gctccgacga cgtacttgac cacctgccac
    16621 ttgccgccgc gcttccggag cagcgcacat acccagtcgt cgaagacgcc gtcctccctc
    16681 gcctgctcgt agacggtgcc cttgtaatcc accgccttcc cgtccggacg ctgcggcacc
    16741 ccgcgcagga aggcccaacc gccctgcacc ttcagatggt caaccttaaa gaccaccttc
    16801 ggcccgagtt ccttctccac cggcacgcgc agcgcgtcga gtatggcccg ccgctcccgg
    16861 tcgccgctcc tgggcgcgga aacctcaccc tgcgccgcgg catcggacag gaacacggtg
    16921 agcagcagaa acaaacacac aatcaaacgt gacaaagttt aagcccccct tcaatctcga
    16981 cgctcaattc catagcgcac ggcgcgtctg gtatcagcga tgacgttctt aaaaacttcc
    17041 gcatagctcg caccgcggcc gagccaaatc ccctggcgcg gccttacggg gtcggggtgg
    17101 agccggcgcc ggcggcgtgc gtggtttgtt ggggggcttc ggcgcgggcg tcggtgagcg
    17161 ggaggcggac gcggaaggtg gcgccgtggc cggggccgtc ggactcggcc gcggcggcgc
    17221 cgccgtgcag ctcgacgatg tggcgaacga tggccagacc gaggccgaga ccgctgtggc
    17281 cgcgctggtg tgcgccctcg gcctgccgga aacgctcgaa gacgtgcggc agaaagtcgg
    17341 gcgcgatgcc ggcgccggtg tcgctgacgg ccacctccac ctcaagcccc acgcgccgca
    17401 gggtgacgga gacctcgccg ccccgcggcg tgaacttgac ggcgttcgac aggaggttgg
    17461 cgaccacctg ccggagtcgg ttggggtcgc ccgtcatcaa cccctcgttc gggtcgagcg
    17521 acgtgtgcag cgtgagcgcc ttcgcctcca gcgaggcgcg cacctcatcg acggccgcgc
    17581 cgatcacctc gtggagatcg agcgggcgcg tgtcgaggtg catcttgccg gagacgacgc
    17641 gcgaagtgtc gagcacgtcg ccgacgagct gcgcgagcgc ctgcgagttg cgctcaatga
    17701 cttcgagggc gcgggagcgt ccccgctcgt cgagcatgtc gagccggagc atctcgacgt
    17761 agcccatcat cgaggtcagg ggcgtgcgta gctcgtgcga gatgatggcg aggaaatcgt
    17821 ccttggcgcg gttggcgcgc tcggcctcag cgcgcgcggc ctgttcgcgc cgcagcagct
    17881 cggcgcgctc gccctcggcg cgcatccgct cgctgatgtc ggccgagacg ccgacgatgc
    17941 cgatcaggcg gcgctcggcg tcgtagatcg gcgagtcggt gacctgtgcg ggaaacgtcg
    18001 tgccgtcgcg gcggcggagg gcgaactcgc ccgaccagct cttaccctcc ttcagattcg
    18061 acatgatctc cgcgacctgc tgctcagtgg cggcggccgg cgtcacttcc aagatgttgc
    18121 ggccgacgac ctccgcagcc gtccacccgt aaagactctc ggcatacttg ttccagtaga
    18181 tgatgaggcc gttgaggtcg gtggcgatga cggcctgctc gaccatgtcg agcagatgcg
    18241 cttggaagtc gatctccgcg gcgacgcgcc ggcgctcggt agattcgcgg tcgagcgccc
    18301 ggttgagacg cgccagttcg gcggccgtgc ggcgcagctc aatctgtaac atcgcctgac
    18361 gcgctaaggt ctggagcgcg cgccgctgtc cctcgtcgag ttcgcgcggg acgcggtcga
    18421 tgacgcagag ggtgccgagg ttgtggccgt cggcggtggt gagcggggcg ccggcgtaga
    18481 agcgtatgcg cgggtcggcg gtgacgaggg ggttggcggc gaagcgctca tcggcggagg
    18541 cgtccgcgac gacgaagagg tcgggcgaga ggatggcgtg ggcgcagaag gccacgtcgc
    18601 gcggcgtctc ggcggcgtcc agccccgtgc gcgacttgaa ccactggcgc tcggcatcga
    18661 cgaggctgat gagcgcgacg ggggcgccgc aaatctgcgc ggcgagccgg gtcagctcgt
    18721 cgaacgactc ttcggcggcg gtgtcgagga tttcgtagcg gcggagcgca tcgagccggg
    18781 ccgcctcgtc cgttggaatc gtgggcttca tcataaagcc gtgaatcgtc aatcgtgaat
    18841 cgtcaatcga aggcttgatg ctctcgcgat tcgtgattgt agcactctgg ccgtgcggcg
    18901 gctcaatcac gattgacgat tgacgattca cggctttttc actgcttgag cacgaaggcg
    18961 gcggggacga actccagcgc gaccatctcg ccgtccgatt tcgcgcgcgc gtctgaggcg
    19021 gcgcggtagg tgacgacgac gaggctttcg ggcttgcgcg ctccgcaact gatctgtccg
    19081 cccgcctcct gtgtgaaggc catgacgtga aggccgtcga agccggcgct acgcagcttc
    19141 agtacgcgcc cgccgacgcg gacgtggaag acgatgccct tcgcgtcgca ttcgagacgc
    19201 gcgagcaacc cctgcgcgcg cgcttccccc gtgagcggct tccgcagggc ctccagcacc
    19261 gcctccgaga gcgcgtcggc caccagctcg tcgggtgtct tgccggtctc ctgcgccccg
    19321 gcgctcgcgc cgcgtctcaa gagccgtggc ggcccctcgc cggacgcggg ggaggcgttg
    19381 tcgtcgtcat gagcactcgc gccggcggag tcgggctgta ggcgcgacgc ccgaccctcg
    19441 aagttgacga tgttgttgag gagcgactgc gcgttggcgc gcaattgcgc gtcggtggag
    19501 ctttgggcga gcggctcgat gagcttgcgc gccgcggcga agtcctgctt gcggaggtgg
    19561 agctgcgcga ggctcagggt gaactcctcg cggccggggg cgatttcgat ggcgcgtttc
    19621 agaagctcga tgctctcgtc gatctgctcg ccggtgacga ggttgacgaa ggcggcgagg
    19681 tgatacgcct cggcgaagtc cggcttgagc ttgatggcct tcgcgagcgc cgcgcgcatc
    19741 tggttggccg cctgctccgt gtagccgccg acgaggccgg tctcgtccat cccctcgcgc
    19801 gagagcgtgt gggcgtagta gtagtgcgcg aggtggttgc gcgcgtcggc cgccacggcg
    19861 cggccgaggt gttctttggc ctcggcgaag cggcgttggc gcgcgcggag cacgccgagc
    19921 gaggcgtggg ccatcgcgtt gtcggcttct aaggcgagcg ccttctggag atgcgtctcg
    19981 gccagctcgg gcaggcgtgt gtgcaacgcg aggtcgccga gatagccaag cgcctgccct
    20041 tcggtgaggg gcgcggcagt catcccggcg tcgaactcca gacgctcctc gaaggggaag
    20101 gtgacgaagg tgtactcgtc gcgccggatg tagtcgcgca gctctttctc gatggccggc
    20161 gcgtccgcgg cgaacgcctg tttgaaactc tcttcagggc tgacgccggc gcgcgccaaa
    20221 tccatgtagc ggttgagctg cggctgacgc tggcgctcct tgccgaacat cagatagtgg
    20281 acgagcgccc acgactgcgc gtagaaaacg ccgcgcttgc ttttttcgtt gtagtgcggc
    20341 gacgaatggt caacggcgaa gagcgtcgcg agtggcaaaa gcttctgctg gcgcaggtag
    20401 aagacgtgcg gcgcgatggg acggccgact tccgcggcgc gcccgccctt cgtcacctcc
    20461 agcgtgctgt aaaactcggc cagcccctcg ttgacccacg cgggcgtgtt cgccgcgacg
    20521 ttgttcttga cgagcaggtg catgtactcg tggaagatga tgccgaaagg gttctccatg
    20581 tcggggccga gtttggcgtt gagcgtgatg tagttgacct cctcgcccga ctggaaatag
    20641 ccggcgtggc cgccggggtt gaagggcttg taggagccgg catccttgaa gacgacgacg
    20701 gtcgtgggga cggcgtcgct cagacgcgcg cgcttgaaga gacgcgagaa gacgtggcga
    20761 aactgttcga gccgcgtcgc cacctgcctg atctccttct cgctcgtgtt gccgatgagg
    20821 aagaagttct tcgagcgcac gctcgtccag gtgtctttag ccgcggcggt cgcgggcagg
    20881 gcggcgagca gcacgagacc cgcgaagaag agtttgattg actgcccgtt catgtgttga
    20941 agtcctcggt gtgaagtcgg ggagagatga ccgggtgggc gcgtcggcac gccgattcga
    21001 gtgaggatgt tacagtccgg ttttagccgc tgtcaaaggg cgtgcggcac agcagaaagc
    21061 ccccggagcg caaactcgcg agggctttca ttatgcagtg ttcagttgag tgaaagtcaa
    21121 tctgattacc agcgcgcgtt atttccgttg gccgccgcgt cgccgcccga gcagcgccca
    21181 cccggccggg agcagcgcgg cggcgagcgt gatcttcacc acgtcgccga tgagaaacgg
    21241 cgcgacgccc aactggaacg cccgcgcggc cgagccgagg gcgaacgcaa gccacgccca
    21301 cccgccggcg aggatgacga aggagccgag caacatcgcc gccgccgccg tcaaaaaccg
    21361 cctgtcccaa ccgcgctcgg cgagcaggcc cgtgacgaac gccgccgccg ggaacgccag
    21421 caggtagccg gccgtcggcc cgagtaaatg accgacgccg cccgcgcccg cgcggaagaa
    21481 cggcaagccc atcgcgccct ccgcgagata ggcgagcatg gcgagcgcgc cgaggcgcga
    21541 gcctaagagc gcccccgtca aaagcaccgc gaaggtctgc cccgtcagag gcaccggcgt
    21601 ccacgggagc gggatgacga cctgcgccgc gagcgcggtc aaaagactga aggcgacgac
    21661 gagcgcggcc gacttcaacc agtccgtcgg cgcgacgaag gcgcccacca gcgtctctgc
    21721 cttagcgtaa gaagtgctca cggaaacctc caaaagccgt aaatcgtgaa tggtaaatcg
    21781 tcaatcgttt tagttgagcg ttcgccgtct tgacgtttat cactcaagag gaataagggt
    21841 caaccacgat ttacgattca cgatttacca tttacgagtt tatcgcacgc ggtcggcaga
    21901 ctgttgcctg acgccttccg tgtccgtgcc catctcgccg gcggcaagct cgccgatggg
    21961 ggtctcgccg ggatgcgccg cctcgatctc cgtgccgata cgcatcccgt agaaggagcg
    22021 ccagacgaag tagaccgaga tgaggaagaa gatggcggcg agcatgtagt tgagcgaggc
    22081 gtcgccgccg cggctgcgat agacgctcag ggccagctcg acggcgagca ggccgaagag
    22141 cgtcgtgaac ttgatgaccg gattcatcgc cacggacgaa gtgtccttga aggggtcgcc
    22201 cacggtgtcg ccgaccacgc aggcgtcgtg cagcggcgtc cccttctgct tgagttcgac
    22261 ctcgacgatc ttcttggcgt tgtcccacgc gccgccggcg ttggccatga agatggcctg
    22321 atacaagccg aagagcgcga tggagatgag atagccgatg aagaagtagg ggtcgatgaa
    22381 ggcgaaggcc agagacgaga agaagaccgt caggaagata ttgaacatcc ccttctgcgc
    22441 gtactccgtg caaatctgca cgaccttctt cgagtcttcg acgctggcct tcacgaccgt
    22501 ctcgtcgaga cggatgttcg ccttgatgaa ctggacggcg cgataagcgc ccgtcgtgac
    22561 ggcctgcgtg gaggcgccgg tgaaccagta gatgatggcg ccgccggtga tgagaccgag
    22621 aaggaagagc gggttgagga gcgagaggtt ttcgtagaag ttggcgagac cgcgctcggc
    22681 cgacaggccc gcggcgctcg cgccggcgac ggcgttgccg cgcaacactt ggatgatcga
    22741 gaagatcatc gtcgtcgcgc cgacgacggc cgtgccgatg agcaccggct tggccgtggc
    22801 cttgaaggtg ttgcccgcgc cgtcgttctc ttcaaggaga tgcttggcgc gctcgaaatt
    22861 gacctcgaac ttgttgtcgc gcgcgatctc ctcacgcacg cccggcacct gttcgatcag
    22921 cgacagctca tataccgact gcgcgttgtc cgtcaccggc ccgtatgaat cgacggcaat
    22981 cgtcaccggc cccatgccga ggaagccgaa ggcgatgagg ccgaaggcga acacggcggg
    23041 cgcggccatc agggcgccga cgccttgtgt gctgacgatg tagccgatgc ccatcagggc
    23101 ggtgatggca aggccgatcc agtaggccga gaagttgccg gccacgaggc ccgacaggat
    23161 gttgaggctc gcgccgcccg cgcccgagga ggtgacgacc tccttgacgt ggcgcgactc
    23221 ggtcgaggtg aagaccttga ccagttcagg aatgaccgcg ccggccagcg tgccgcaggt
    23281 gatgatcgtc gagagcttcc accacagcga gtcgtcgccg ccgagcgtgg ggatcatcag
    23341 gtaggagacg acgtaagtga gcacgaccga gacaatcgag gtgatccaga cgaggctcgt
    23401 cagcggcttc tcgaaattca tcgtctgggc gtgctcgtag cgcgacttgg cgatggcctc
    23461 gttgacgaag taggagagcg ccgaggcgac gatcatcatc acgcgcatga cgaaaatcca
    23521 caccagcagt tgcacctgca cggtcgcgct cggcacggcc agcaggatga aggtgatgag
    23581 ggccacgccc gtcacgccgt aggtctcgaa gccgtcggcc gaggggccga cggagtcgcc
    23641 cgcgttgtcg cccgtgcagt cggcgatgac gccggggttg cgcgcgtcgt cttccttgat
    23701 cttgaagacg atcttcatca agtccgaacc gatgtcggcg atcttggtga agatgccgcc
    23761 ggcgattctg agcgcggccg cgcccagaga ttcgccgatg gcgaagccga tgaagcaggg
    23821 gccggcgtag tcgcgcggga cgaagagcag gatgcagagc atgatgagca gctcgaccga
    23881 gatgagcatc atgccgatgg acatcccggc cttgagcggg atggcgtaga gcgggtaggg
    23941 cttgccgccc aggctcgcga aggcggtgcg cgagttggcg aacgtgttga cgcggatgcc
    24001 gaaccacgcc acgccgtagc tgcccgcgat gcctaccagg ctgaagaaca ggatgatggc
    24061 gacgcgcgcc gcctcgaagt ccagcaggac gccgaagtat aaaatgatga cgagggcgat
    24121 gaaggcttcg aggatgagga tgaatttccc ctgcgtgatg aggtatgttt tgcacgtttc
    24181 gtagatcagc tcggagatgg tgcgcatcga gtgatgcacc gccatgctgc ggagtcggaa
    24241 aaacatcacc aggccgaaca ccgcgccagc aaaacat
//
LOCUS       DASVES010000446         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1639592, whole genome shotgun sequence.
ACCESSION   DASVES010000446 DASVES010000000
VERSION     DASVES010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639592"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..2850
                     /locus_tag="VF240_18425"
     CDS             334..2850
                     /locus_tag="VF240_18425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177239.1"
                     /translation="MKQAFRLSWSGALALLLLSGMAATSARAQSTSATPDSVVVQIAN
                     TAPTPPFTPAPVASPSPTPVPNLNRDTYAQDVSGDGRFVVMESTGDIATERTATRNNI
                     DGNVEIFLFDYAQRRIFQITNTTNALKDAAGSPVVSANIDASVRNLRPMISHDGRYIV
                     FASNAYSDANSALTPKNFDGTANAAALKADGNMEIFLYEIPAVGAALLSSGAEAPQAD
                     LVAGTMTRVTFTAASRVATPGTATVAPFVADDNRAATLNDDGSFLAFVSTRNIANVGG
                     ASNADANPEIFLWNRTSPSFIQATVTSGAFSFNDNPSLSGDGAVLAFFSTADIGSNET
                     QADRGNGELYAASFNGTTVSNLRQLTRTPNNANGDPVNILSPGRRLSRNGQLLAFEST
                     AVFNSNGSLNGALADNFGIYFVRVADATFTLVGSRAPTGQLVDLLRFPTFTGDSANIV
                     FSSTLNLRATTGEALAEDSADGLNGGTSGDANITTRRRTQVYAVPVPAAGAAPANYQR
                     LTRLPTRFGAAQVFPTDTLNRLAFTLQFAELGGGNSDSGYEAYYLIVPGVTGEASSAT
                     VAFSTGASDRPVVSASPAPTPPVVGGLAPGMLGIARSATTLAPSNTQVSQNDATESGA
                     NARRPPLPIELNGVTVTVSGYAAGLFFVSPGQLNFVVPPGLLIAAANTSTPVVINNNG
                     TVIRATLALNPAQPDIFTTSNGDGGRAAVLNVTNPCIAAPGEPFTVTTTRPTGGDCAA
                     ATTEQVATQLLIMLTGARGPLGTPTLRTEVSVTIGTTTLPADAIVSVGPSRTHGFDQI
                     IVTLPATLAGAGDVPVIVTITKGGVTTSRPADTAPRITIQ"
     gene            2950..>3452
                     /locus_tag="VF240_18430"
     CDS             2950..>3452
                     /locus_tag="VF240_18430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177240.1"
                     /translation="MLFGLDGIPLTLPKTGVGHYTAELARALAAAAPADTFELVYPSS
                     EAPPEGALDPLPARPPNLRTRRVEVGVVGRHWWALGLPRFARREGAYDLFHGTNFDVP
                     LWPGRPTVLTVHDLSTLLHPETHTPRSVRRARRRLPLMTRVATVIITPTEAVRREVCE
                     RLSVPAA"
BASE COUNT          537 a         1378 c         1014 g          523 t
ORIGIN      
        1 actcttattc atccctcatc cctcatcctt ccgccttgtc cggcaacctt cggcgggggg
       61 cggcgtcttt agtttcaacg tgtggcgcgt tggcctcggc cggcgtcggc cgggacggcg
      121 ggaaaagttc ggcggtttga gcatttacgc cgcgggggcg gccccgacgg gccggcttgc
      181 gctcgtcggg ggtaaccctt aaaatgcctc tctttgactc cgcgcgagcg cgtcgcaaac
      241 gggtgccgcc ggtagtcgcc ggccgtcccg cacgtctccc gtggtaacgc ccaaagccag
      301 ttctagtttt cgttccggtt cggaggattc ttcgtgaagc aagctttccg tctgtcctgg
      361 tcgggcgccc tcgcgctcct gctcctctcc ggcatggccg ccaccagcgc gcgcgcgcag
      421 tccacgagcg ccacgcccga cagcgtcgtc gtgcagatcg ccaacaccgc gccgacgccg
      481 cccttcacgc cggcgcccgt ggcctccccc tcgcccacgc ccgtccccaa cctcaaccgc
      541 gacacctacg cacaggacgt gagcggcgac ggccgcttcg tcgtcatgga atcgacgggc
      601 gacatcgcca ccgagcgcac cgcgacgcgc aacaacatcg acggcaacgt cgaaatcttt
      661 ctcttcgact acgcccagcg ccgcatcttc cagatcacga acaccaccaa cgcgctcaag
      721 gacgcggccg gctcgcccgt tgtttcggcg aacatcgatg cgtccgtgcg taacctgcgc
      781 ccgatgatca gccacgacgg gcgttacatc gtcttcgcct cgaacgccta cagcgacgcc
      841 aactccgcgc tcacgccgaa gaacttcgac ggcaccgcca acgccgccgc cctcaaggcc
      901 gacggcaaca tggagatttt tctctacgag attccggccg tcggcgcggc cctgctgtcc
      961 tccggcgccg aggcgccgca ggccgacctc gtcgcgggaa ccatgacgcg cgtgacgttt
     1021 acggccgcct cccgcgtggc cactccgggg acggccaccg tcgccccctt cgtcgccgac
     1081 gataaccgcg ccgccacgct caacgacgac ggctccttcc tcgcctttgt ctccacgcgc
     1141 aacatcgcca acgtcggcgg cgcgtcgaac gccgacgcca accccgaaat ctttctctgg
     1201 aaccgcacca gcccgtcgtt catccaggcg accgtgacct ccggcgcctt ctccttcaac
     1261 gacaacccgt cgctgtcggg cgacggcgcc gtgctcgcct tcttctcgac ggccgacatc
     1321 ggctcgaacg agacgcaagc cgaccgcggc aacggcgaac tctacgccgc cagtttcaac
     1381 ggcaccacgg tctctaacct gcggcagctc acgcggacgc ccaacaacgc caacggcgac
     1441 cccgtcaaca tcctcagtcc gggccgccgc ctgagccgca acgggcagct cctcgccttc
     1501 gagtcaacgg ccgtcttcaa ctccaacggc tcgctcaacg gcgcgctcgc cgacaacttc
     1561 ggcatctact tcgtccgcgt cgccgacgcc accttcacac tcgtcggcag ccgcgccccc
     1621 acggggcagc tcgttgactt gctgcgcttc ccgacgttca ccggcgacag cgcgaacatc
     1681 gtcttctcct cgacgctgaa cctgcgcgcc accacgggcg aggcgctggc cgaggactcg
     1741 gccgacgggc tgaacggcgg aacctccgga gacgccaaca tcacgacgcg ccgccgcacg
     1801 caggtctatg ccgtccccgt cccggcggcc ggcgcggccc cggcgaacta tcaacgtctg
     1861 acgcgcctgc ccaccagatt cggcgccgcg caggtcttcc cgaccgacac tctgaaccgc
     1921 ctcgccttca cgctccagtt tgcggaactc ggcggcggca actccgacag cggctacgag
     1981 gcttactatc tgatcgtgcc cggcgtgacg ggcgaggctt ccagcgccac ggtggccttc
     2041 tccacgggcg cgagcgaccg ccccgtggtc tccgcctcgc cggcgccgac gccgccggtc
     2101 gtcggcggac tcgcgcccgg catgctcggc atcgcgcgct cggcgacgac gctcgcgccg
     2161 tcgaacacgc aggtcagcca gaacgacgcc accgagtcgg gcgccaacgc ccgccggccg
     2221 ccgctcccca tcgagctgaa cggcgtcacc gtcaccgtct ccggctacgc cgcgggcctc
     2281 ttcttcgtca gccccggtca gctcaacttc gtcgtgccgc ccggcctgct cattgcagcc
     2341 gcgaacacga gcacgcccgt cgtcatcaac aacaacggga cggtcatccg cgcgacgctc
     2401 gcgctcaacc cggcccagcc cgacatcttc accacgtcga acggcgatgg cggacgcgcc
     2461 gccgtgctca acgtgacgaa cccgtgcatc gccgcgccgg gcgagccttt caccgtcacg
     2521 acgacgcggc cgacgggcgg cgactgtgcg gcggcgacga cggagcaagt ggccacgcag
     2581 ttgctcatca tgttaacggg cgcgcgcggc ccactgggga cgccgacgct gcgcacagag
     2641 gtatcggtga ctatcggcac gacgaccctg ccggccgacg ccatcgtctc cgtcggcccc
     2701 tcgcgcacgc acggcttcga ccagatcatc gtcaccctgc ccgcgactct cgccggcgcg
     2761 ggcgacgtgc ccgtcatcgt caccatcacc aagggcggcg tcacgacgag ccgcccggcc
     2821 gacacggccc cgcggataac aatccagtga gcagtaagca gtgagcagta agcagtaaaa
     2881 acctccggcg gcaagcgtgc gctcgactag cggcactgct cactgctcac tgcttactgc
     2941 tcacttttta tgctcttcgg actcgacggc atcccgctca cattgccgaa gacgggcgtc
     3001 ggacactaca cggccgaact ggcgcgcgct ctggccgccg ccgcccccgc cgacacgttc
     3061 gagcttgtct atccttcgtc cgaggccccg cccgagggcg cgctcgaccc gctccccgca
     3121 cggccgccga atctgcggac gcgcagggtc gaggtcggcg tcgtcggccg ccactggtgg
     3181 gcgctggggc tgccgcgctt cgcccgccgc gagggggctt acgatctctt ccacggcaca
     3241 aacttcgacg tgcccctgtg gccgggccgc cccacggtgc tcaccgtcca cgacctctcc
     3301 acgctcctcc accccgagac gcacacgccc cgcagcgtcc ggcgcgcacg ccgccgcctg
     3361 ccgctcatga cgcgcgtcgc caccgttatc atcacgccga ccgaggcggt gcggcgcgag
     3421 gtgtgcgagc gcctgagcgt tccggcagcg aa
//
LOCUS       DASVES010000447        34467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1478666, whole genome shotgun sequence.
ACCESSION   DASVES010000447 DASVES010000000
VERSION     DASVES010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 34467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34467
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478666"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..778
                     /locus_tag="VF240_18435"
     CDS             425..778
                     /locus_tag="VF240_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX7177241.1"
                     /translation="MTTDQNCLFCKIVAGEIPAQIVHTDDHTVAFRDINPQAPTHVLV
                     IPRRHIASLEEATQSDESVLGRLLLTAARVARDEGLAEGGYRTVINTGAGAGQSVFHI
                     HAHVIGGRPLAWPPG"
     gene            841..2508
                     /locus_tag="VF240_18440"
     CDS             841..2508
                     /locus_tag="VF240_18440"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7177242.1"
                     /translation="MKNLICALVTLALVLLASPRTPRAQAGPQQSSGAYRMFLGAQEI
                     GTETYVMSAEPDGTRRAEAELVFGVNRSKATTIAAPNRPVSFTVEAGGARLLAVEFTA
                     DGAKVTAAGQTPRELKTAATVILENAVWHHYNYLLAQYDAGRGGAQQFVAFLPSQATQ
                     FTLQLERVGTPTFTVGGRQVATEHYRATMGQGLSVELWADAARVPLLIRVPAQGVRVV
                     HVGSEALAEAISPSAPAAPPATAANEAYTSEEVTFKNGDVTLAGTLTLPRRGAAPHPA
                     AVIITGSGGQDRDGSNVLNLYRRIAEQLSASGVAVLRVDDRGMGKSVMPKMTSVSYRD
                     LINDTRAAFDYVAARREIDPKRVALVGHSEGAQTALSIAAEDGRVGAVALLAGASRPV
                     NHIVYEQALYMIALGEAIDPSDAMKLPQISRDLLKIFDDLKTQPAPARPAADKYAWFR
                     EHAASNPMELARRVRAPTLVLNGERDSQVLPYHALELAQALAAGGNRQVRLRIFPNLT
                     HLFTPSALDKSVTGERSAEVSEEFLKTLATWLSGVFGAGGRGERVKG"
     gene            2710..3738
                     /locus_tag="VF240_18445"
     CDS             2710..3738
                     /locus_tag="VF240_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177243.1"
                     /translation="MSTETLTPFESFLNEHDADAWQGAVAELLPHVHEVDRNAVQIWF
                     AFFPLALLRAFQQSEDPERLAGALQMQGRWLLADQIDTSHTFLYGHRYWPEVRRAVAA
                     LAEAGDGQTDASLAGRIRELAARVASELKVEQSLVVGIAAVACMTLQQAGLEAFKSAP
                     GTVHIDKKYLKKTPEQVLSARARDDRQGLFGFLRTEDKLWTVVWDEHDDERRFKAMHL
                     QEVASGAAEDKRPWHELDTRCTVDEGPIPVQCRSASCGTCWVGVLGGAEKLTEVAARE
                     RRKMPEMGYIDTDEARPLIRLSCQAQATGALSIVIPPWNGFFGKYLKRQREAAAVGET
                     VQTSEATA"
     gene            3750..4310
                     /locus_tag="VF240_18450"
     CDS             3750..4310
                     /locus_tag="VF240_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177244.1"
                     /translation="MGEDTGAGGAGQHQEHRPPSLRRDAEGRVVPSTLAELIQWFLDY
                     DERVAVVRFPAVESLFQWKQQEALKAEPDAFAFNTAEDRLAVGVMQALQTYQTERALH
                     DWLKELLGALDDATKTNEAILEAYGLHYGEEHSVIEEAEKIPTRRERDVYLTCCWLET
                     LCTAEARVLGWAYQELYGRPFHPQNF"
     gene            4361..4630
                     /locus_tag="VF240_18455"
     CDS             4361..4630
                     /locus_tag="VF240_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177245.1"
                     /translation="MAKEPTNYTNTSQTVVVDADTFEFETLEENNGVATVVRFRVENE
                     DVRAGDVLLVLSGTDIHFHGMIGRIEEGHGTATDRRGSLLPATVQ"
     gene            complement(4939..8520)
                     /locus_tag="VF240_18460"
     CDS             complement(4939..8520)
                     /locus_tag="VF240_18460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX7177246.1"
                     /translation="MGSARTTLSKPRPHALLSVTFAFCLFTVAFFCALAQKRDAEESP
                     AQAATFHRWGAVTLFHGLPSDRVRAIAQDAEGHLWFGTDAGLARYDGRRTQTVTEAGL
                     AAARVLALRADEEGALWVGTDDGAYLRTSAGEFKSIPETKGKRVAAIITPTRGRAVVA
                     TSDGLVFDCRRKDDGTYTAEPLAEQILPAQPPPGTSSSASTSSTRPPLELTSLAAAGE
                     TILVGTRGRGLLSVERGGAVREVVSRPRAFFFEALERDGAGRLHAGAQAPASESGIFR
                     VDESDAAAAATADASSARAAKVAGEATGTVNALRFDARGDLYVGTDGRGVFHMREGRR
                     IERFTFAGTAGALRSDRVYSVFIDREGVAWFGTDRGVCRYDPRGLRVENVAEELDGNF
                     VRALYRTSSGRLLAGTNRGLYVREREGIAWRAVEEIGRRTVYSIAEDRRGRVLVASAG
                     GLYASLAPDKSAAPGTIRFKPEEKKDEGGDEAEDGAKTKAEQAGAVAADGAVVAAAPT
                     ATPAQQSAEGQPEQKVAAAPERVEPEKPVSRPVAPPVVAGTIRAVAVLGGVTYIATWG
                     RGVERLDEELRRTILWPRSEADNARGQEAISLHADEQAGRLWVGTTGGVFYLDAEGGV
                     KTEPAHEPLGASTVYGVSSGPGGWLWLATNRGLYAQAPGGALVEAAPGRDARSVLAAL
                     PQTVAAVGGDAAAAAEASRPAPPQAWCATAGGGVIKVLLDEQFGPVTAALDAEQGLPS
                     QNAFALLSSTLPREDGDEQTLVVGTSRGIALYGPGAAPPVIRPVQVVAARPLALEDLE
                     GAGRDGERRGLYLAYPQNGLVLDVAANSSRTFPEQFQYAFLLTDDAGKTITRKLAHDA
                     QFRVEGLRPGRYRITARAFTIDLVASAPFGFEFVVERAPFPWTITALSVLLALSLVAL
                     VWGYVQHRKIVRAGSDLLEANRQLAAARLQLANEAESERRRIARDLHDQTLADLRRLM
                     LLADEMRPAGATEAPAPATTAPAAMATAEVSGGGAHAPDAPARTLAPALDPAVLRAEI
                     ESVSQEIRRICEDLSPSVLENVGFAAALEWALAERVAHMPPDCKFIYEFSCDEHLEER
                     LHFAPGVQMQVYRIVQEAVSNVCRHAAARRVRLFVSATDEGVFHLTLEDDGRGFDTDR
                     RKSRGERGLASIRARASMIDAEVRWQRRPDTEGGGTLFTLRKEPAAT"
     gene            complement(8553..9314)
                     /locus_tag="VF240_18465"
     CDS             complement(8553..9314)
                     /locus_tag="VF240_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7177247.1"
                     /translation="MSILIAEDNVAQRHYLRELLEREFPKYAPVVEASDGEEAVRVAL
                     AQRPELCVFDIQMPRMSGVKAARTIWREFPGARIIFWTQFGHDVYINEIRKIIKSLEP
                     QPAYGFIHKNNPESRFLRFVAAVLEDGADMIDPAFKDSFKQPLLTEFEAEALYFLALG
                     LSNWAIARKCSLSLRGVESRLATLYEKLFISTPEGTPNEAYDKLAYNMRTRAFFEALR
                     RGLINSDELEKAAADLDAWIERDRKRFTEEQKTHK"
     gene            complement(9892..11355)
                     /locus_tag="VF240_18470"
     CDS             complement(9892..11355)
                     /locus_tag="VF240_18470"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7177248.1"
                     /translation="MRKRFASSAVLAFALLLCATAAQAQAPAAAADKAANIDELVKSY
                     HGIRQFNGAVLVAEQGRVIFRKGYGMANMEWAIPVEPDTKFRLGSITKQFTSALVLQL
                     VEQGKIKLDGKLSDYLPDYRKDTGGRITIHQLLNHTSGVPSYTGRANFLKEESRDPYA
                     VSDFVKKFASGDLEFEPGSKFAYNNSGYFLLGALIEQVTGKTYEQVLKERILDPVGMK
                     NTGYDHHGTLLAKRASGYAKTPTGYDNAPYLDMSLPYAAGSMYSTIEDLYLWDQALYT
                     DKVLSAKTRELMYTPGLRNYAYGWGIQKTPLGGKETTIVQHSGGINGFATLIVRIPES
                     RHLIVLLDNTQGAKLQQVAQNIARVLYGMPTQPAREDISEVLFQTITAKDVTAAVKQY
                     RELKATRAAEFDFAEPQLNQLGYQLLQTKRVREAIEIFKLNVEMYPQGFNAYDSLGEA
                     YMVDGQKELAIQNYRKSLELNPQNTGAVEALKKLEGK"
     gene            complement(11576..12226)
                     /locus_tag="VF240_18475"
     CDS             complement(11576..12226)
                     /locus_tag="VF240_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177249.1"
                     /translation="MMSGKPEWEQFTAREWEIVALHRTPARVQRYLSRLPYNWEREGG
                     TLRSFREVVRRGEAHCLEAALAAAVILEQHGHPPLLLSFESQDLLDHVLFVFRQKGLW
                     GAVARSRDLGLHGRRPVFPNLRQLVWSYFDPYVDHTGRITGYGLADLRDLGGYDWRFS
                     PRNVWKVERHLQQIPHQHLSSSDARYQKLLARYKEFKRRHPDRHLTDYRGRETWMI"
     gene            12645..12887
                     /locus_tag="VF240_18480"
     CDS             12645..12887
                     /locus_tag="VF240_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177250.1"
                     /translation="MSRSQTSSSGPVRSPAELETRSAAERLSAQIEDALAAVAGHSND
                     STEELEACADRLERAARDLAAALRELASRRAGAAEE"
     gene            13138..14766
                     /locus_tag="VF240_18485"
     CDS             13138..14766
                     /locus_tag="VF240_18485"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX7177251.1"
                     /translation="MVATRQQAARELGRRRSAVGLDAYGIQPAYDVYWNLTPPELYEE
                     VARRGEGRISQHGALLVDTGEHTGRAAKDKAIVREPGSEDKVFWGDVNKEFPAERFNA
                     VRDRMMQYASSRELFVQDTFAGADPRYRLPVRVINELAWHSLFARTMFISPEESARED
                     HTPEFTIINLPSFKADPATDGTRSDTFILMDFSQRLVLIGGTSYAGETKKSVFTILNY
                     LLPQRGVMSMHCSANVGQQGDVAIFFGLSGTGKTTLSADPERRLIGDDEHGWSDDGVF
                     NFEGGCYAKVIKLSAEAEPDIYRTTRMFGTVMENVVYDPVTRVPDLDDSSKTENTRAA
                     YPLTSIPNIVTEGHAGHPSNIIMLTADAFGVLPPVARLTPEQAMYHFLSGYTAKVAGT
                     ERGVTEPQATFSTCFGAPFMVLHPGVYADLLGRKIQEHKAACWLVNTGWSGGPYGEGK
                     RMSIKHTRAMIRAILSGTLAAVETRPDPVFGVGVPLSCPDVPAEVLTPRNTWKDKDAY
                     DAKAHDLARRFNDNFKKYEAGVSAEVRAVAPKAG"
     gene            complement(14909..15493)
                     /locus_tag="VF240_18490"
     CDS             complement(14909..15493)
                     /locus_tag="VF240_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177252.1"
                     /translation="MNTNGIIRGILFTAAIFIAGVGAHAQQAETQHVTVTVRPAQSAE
                     ERVAEEFKEKALRKAAEEKLAAEESAKIAEASPKALLRRARTIYIESGTSFFEPVQLQ
                     NALSKRAEMDAWQMAIVDGWQKREVADVVIEVDRPVFTYTFTYRVTHRGTGVVLATGK
                     VTAFDGNAAAPMLAVKIVEEIMSARGETKTKRKD"
     gene            complement(15556..16104)
                     /locus_tag="VF240_18495"
     CDS             complement(15556..16104)
                     /locus_tag="VF240_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177253.1"
                     /translation="MKRVVEVFGWLAAVLIFALAGLSVLAAQTTGRQTQLPPSQSRAR
                     QARETAEDASPVAALRAARKIHVRPGRHVDAKYLEYKLLKHAEFRDWGFVVVEEERAA
                     DLVIKIDKTMLNYIFSVVDPRTSAVVVGGKVVAINKQLAAEYLGTEIIKKMRQVRASS
                     DNTSPRRKKKAADEEDDEWSDS"
     gene            16491..18167
                     /locus_tag="VF240_18500"
     CDS             16491..18167
                     /locus_tag="VF240_18500"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177254.1"
                     /translation="MAETSEDSLRAEGARSSAVGAGVGRPDGAATLRTVVEMVRRVMR
                     ADTAGVASFSLDERTITWKAMSGFRSVAAEGEREIVTPLRGEFAERAAAVGESIIEVR
                     GLAGDLPVSEFPLHSAEGVRDLALVPLSARGETLGVLAVGFRTSHRFAPEEKQLLEDL
                     AEMAALALDNARLFETIAAAKKIWEQTFDAIPDGIIVHDEQMRVTRCNLAAAEMMGFS
                     APAEAIGMSVASAFARLFGERAAAYHMRQGAVARTASAFELQAEDGRRYLVSVSPLMS
                     METAGWESDPDTTGTTEPAPRTTWSVISWSDITELAEIQEQLARSRRLATVGQLAAGV
                     AHEINNPLAAIAACAEAVVRDVRASEPASALAAEGNWNFYLEEIVRQALRGKAITRGL
                     LDLSRQRRAKREAVELNQLVERAARLFEPRGAERGVRVELDVDARVGEVATDEGMVRQ
                     ILDNLLSNALDAAGAGGRVGVSTVLDAARVRVEVEDSGPGIPPEILARVFDPFFTTKD
                     PGHGSGLGLAISLSLAEAIGGALTVESKPGAGTRFRLWIPRRSPESNKTQ"
     gene            18272..19717
                     /locus_tag="VF240_18505"
     CDS             18272..19717
                     /locus_tag="VF240_18505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7177255.1"
                     /translation="MSRKIRVLVVDDDAVLRRVVSEQIAKAGFDARAAGSGEEALKLL
                     GDADLDVVLLDIRMPGLSGLDALREIRKLDDPPEVIMLTADTSLGTGIEAMRHGAYDY
                     LTKPSTLDEIEAVIRKADEKRRLVRQNAGLREAVRARGGGGGGNSDDAPALVFNSRAM
                     GDLVALAETAARSDSTVIITGESGTGKDVFARFIHARSARSAQPVITVNCAALPEALF
                     ESEFFGHERGAFTGAGAAKPGLIEAADSSTLFLDEVGETAPATQAKLLQFLEEGRFRR
                     VGSTRDRAADVRIIAATNRRLAEEVAAGRFRSDLFYRLNVISLHVTPLRERAEDVPAL
                     VEHFLARNRERFNRPALDLSDEARRRLESYDWPGNVRELRNLLERAAALATGDRIEAE
                     QILPARDNSTDTAAANANANTSPDTSTSAQQPPATLEELERQHILRVLHETGGNRERA
                     SAVLGISARTLYRKLREYETDAAPGALATED"
     gene            complement(19723..19956)
                     /locus_tag="VF240_18510"
     CDS             complement(19723..19956)
                     /locus_tag="VF240_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177256.1"
                     /translation="MTRRKRNAAYALCVNNEGYEASLELRKLYQILPPEPNDPEGWLR
                     VVDESGEDYLYSGERFAPLTLPREIKQVVEATF"
     gene            complement(19960..20199)
                     /locus_tag="VF240_18515"
     CDS             complement(19960..20199)
                     /locus_tag="VF240_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177257.1"
                     /translation="MLEFRRIIGPVTVVENIAVGGKIREVERLRRRYGKGRWRKVRGL
                     ASVELTDGEVHRAELHWYEAHGIGRKEMKVKRLLD"
     gene            20566..20913
                     /locus_tag="VF240_18520"
     CDS             20566..20913
                     /locus_tag="VF240_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177258.1"
                     /translation="MAAKLVAALRAAGHEADTCQHTMDVLRAAAGGRFDLIAVGLDMA
                     GFGRAGAVEALREIAPNTSLIALHDRPAEIMRAATLARVAAVLPRPVRVKDFMYAVAC
                     ALNEQQSVMADPV"
     gene            21048..21560
                     /locus_tag="VF240_18525"
     CDS             21048..21560
                     /locus_tag="VF240_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177259.1"
                     /translation="MRKIGTRTMLALCVVALCGVAAMSFARGRTAAAGRPEVKVALAG
                     TVEREGKQVALAAAESVKPGEVLNWQIATVNEGTGAAREYKTVGEIPAGTAYVAGSAA
                     GENGSTVTYSIDGGKTFSTQPIIEQKQPDGTVKRVAAPVSMYTQVRYEWADELAAGGR
                     VNASYKVRVK"
     gene            21660..23777
                     /locus_tag="VF240_18530"
     CDS             21660..23777
                     /locus_tag="VF240_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177260.1"
                     /translation="MNHILHTRLPHGRALFAVLALLVLALGAPPARAQQTTGGTVISN
                     QASASYSDGTNSYSVNSNTVTVTVANVASLTITPDGGSVPTVVAGQTGVDFTFTVTNS
                     GNFATQVRFPASGAAVVPSAGLTVTQAVIDVNGNGIDGGDIDILGNGSAVLYPSASPY
                     LARNASFNVVVRTTVDAAAAAGATVGVTLGDASGDNVASNGSAAEVRTSVPSGVTLTG
                     SESEAIGSITTTVQNDAQLRLGLSAPAGPVALGSNVTYTWTLDNVGARAVAPRTLTNA
                     PAGSQTGVFVIAPVPSRTTFSSVTPPAGVTVLYSTSALTTDPVTGAVWTNVAPPPGSV
                     TRVLFNVGAALAPAASVANMQMVVTVNTGINATLPLYEIGDAFGRNSVNALLTDQSGD
                     LVVNKGDGNADFNEPRLGVDPLSATQGFQLPTLLTQVGSVLFGPSGAPAAVGPTDNND
                     DYTNRTVAPAAIAGLSHLDTLSSATTVDFTNTVQNTGNSDDTFTFTAPTAPAGFTVQI
                     STNGGTSFTTLSGGGSTTLAVAFGASANVIVRVTAPAGTAILQTGGFNTVVRATSGTT
                     PASSNDTHDNYYTGFLKLVKTVLVFNSTGIGGASDPVPGADIEYSITYSNVSVGGGGA
                     GCVDLVASSVVISEDGNAAPNNWGTTTTQLLVPAPSDSNGGAITDGNTNGAVTVATTF
                     LRDAAGTVNPGASYVFVFRRRIN"
     gene            23888..29683
                     /locus_tag="VF240_18535"
     CDS             23888..29683
                     /locus_tag="VF240_18535"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX7177261.1"
                     /translation="MSLRRPRRIARALAITLIAASVMGAHARPARRDGADTGTVISNR
                     AEAVYHDADGASFRTVSPTVSVTVLAVSGLVVTPDETEASAAVSPNERVTRLFRICNT
                     GNTPDIYTLTRADVSAPATLHTLHFDVDATGTLTDGDRAVQLNTTMSPRLARGECTGL
                     LAVVDTNASQPQSQINISIIARSNVLGTVNGAVEDTGTIINAVGNGARLSSPDDAQLP
                     PSKLVEGHDRITAAHAQTLNYLVSFRNSGDVTARRVLLRDDLPEGLDYVAGSLRLGTR
                     NLTDAEDADEGLFRDRHFEIRLPEVKIDETVRVAFQARVTTRVAPGEGVVNTAQISAD
                     NAALVASSTATAVVNPFGLVYEGRGGAASVIPGARVALLADSTTGATVSLPTGGAVPN
                     ESNTNPYLSDGQGRWSFALSPAQLGAADSAARYFLNVTAQGFRSRMIEATVTPQPGSG
                     GLFTLLVRALDGQPVAQAGGFELTTEDVSIPRLAAFALNVPMFENSAIEIQKTADRPA
                     AEVGEVVTYRVEVRNATDSAVSDTVVNDTLPPSFHFAQGSARLDLPPAGPRPIEPEVA
                     SGGLLIFRLGTLPSGARATLVYRVRIGANASEGEQFNSASAVGRVAITGERLTTEPAR
                     APVRVRRGIFSTQQIIIGRVYEDANANGAFDSGERALAGVRLYLNNGQSVITDSAGQY
                     NFPTVGEGSHVISLDPLTLPPGYAISNANRREQTSWTRLLRTPLGGGAMLRQNYALLP
                     PADSAATTTQAAPRSASAGASVDATASASLNYAQSGAAPRRGDGQQSADKSAAGESSN
                     GANGATAAAETSHASNGTEAARAPGTFEVATTETVEPVAPGKVKVLSPAPGVVVAGAA
                     LEIEARVANDWKVSVEVEGQSVSEAKIGERRVDHKNKVTTYGFVGVSVRPGPNRVTVT
                     AIGPDGRAGETVELTAYGRGPAKRLEIVSDKAELSAGGRDRTTLRVRAFDQWGHPAAD
                     GAVALEVSAGRLLRVAPAEGSKGTSEEARRAAEGVGSIAAGHEASTEAQAAAEAKSQI
                     ITLSGGEAVAELVADGAPGMVEVHATTGALAADARVRVTPEIRPTILVGLAEVSVGSA
                     APEISMHGSQERARSRIAFFYRGPVFGSKNLLTLAYDSQRPLNRTSGSDRMFQLDPLE
                     RAYPLFGDSSTRYEDAQSNSKLYARLDRGRSYVMFGDFDTENRDAGLASYSRRLTGVK
                     LHVENSKGDFVSVTGARPDTAFARDVFPGGALGFVRLTHADVMPGSEVVAIEVRDRRN
                     PEVIISREPLVRSVDYNFNATTGEIFFLRPISTFDYALNLMQVVVTYEHRAGGMSSAV
                     YTGRVVKNFEGAGLKVGLSVVEQQQSEFGSFVVGGIDAEKKLPAGGRVRAEWAMSRGR
                     VLFSGNLFSAGGGETDSRHDGSAFRAELEQPLPWRETRLRASFLRADEGFMNPFGATV
                     TPGSRRAEVSLDMRVRPSSALRLGVTDERNKTANVNNSRLTGSVQWTETVKDRLRATV
                     AYDYRHFEDELGGREVNSNLLTVGAEWQATDKLQLSVRREQNLSEADPTYPNQTTLAA
                     NYQWSQFTRLFFTQRLGSAAIVPIGDFSSTGFAVSGARRETAVGIETRLGRYANLVSR
                     YQLENGANGTDSFAVIGLQNRFALTKELQLDLGYERGFHLAGQGQSFNNLHVGFGWQP
                     TANFRSSARYELRDRGGVGNVLTLGAAGRLADNLTTLARYQLSRANFQGRANSSMSAQ
                     AALAWRPLHTDRAGLLFSYTRRQLEQEGANNSGSTLDRADIISADGFYQLTNDLELYG
                     RFALRYSDTGTPELVRVSSLAYLSQARAAYRMGRYFDLAAETRMLIAPSSGTRRNSFG
                     AELGYWVLPDLRFGGGYNWTGATEPTGGALIGGRRGFYFTISSKLSNLFDLFGASRAS
                     EQQIVEPDDAPDGQGKPKKEDDEEEDEEGGN"
     gene            29697..31043
                     /locus_tag="VF240_18540"
     CDS             29697..31043
                     /locus_tag="VF240_18540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177262.1"
                     /translation="MRRAAQTLSWFVGRRLRAAAPAPRAARTSSARRWALSLLFALLG
                     FQAAHASLSVTPVTWNVIGLDSNNVNSGPNTYLVGVLVCNTGGAAVTNLTGTLVWDSS
                     NIYINTVGLNPVTYPSLAAGACTNVYFNVAVTRTTFAYNTARRYHIEITATGEPVYST
                     PTPREIYVEKLVSQNRNQTLSISGPTSLIVGQTYTYTVTTKTATGGYEQLETFLNFPN
                     TIFQVLSVAVTYTAPAGATNNKIYADACGWDNDPTHVAPNPIQTYRSCIGPVNYPGGK
                     AGDQVVTTYTVKVVGNGTATMNSMIYDFSGSSYHYSNDYGPQSLTLTSQTPPTVPLVK
                     SVSPNGSQPPGADLTYSIAFNNTGDLAAQQFVITDPVPADTDFKIGSVTTTLGSLSGV
                     TIQYSTDGVSWSAALPPPTGTEPAGYNRNVRHVRWVFTGTLAAGSSGAGAFTVRIR"
     gene            31198..32337
                     /locus_tag="VF240_18545"
     CDS             31198..32337
                     /locus_tag="VF240_18545"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7177263.1"
                     /translation="MSTEGDGKRLTDLTVGDARRALVYAAATLAAVVLFLALVWEVAV
                     ALMLGVVVGAYLLPLQEFFERRLRRSTGSALVTMAVIIIPLVLLFGYGWYEMSGYSTV
                     VVERRDEIADSISRSLARYLPLQYEDTRASLQTAFTEAVVRSGQAIGAVREKTALIIT
                     AATLFFFTIYYVLTQRMQLEAYIKLRVPGDFLPLYEKLEESVGGALRGAVWAVFIDQT
                     VKASVIFVCNLVFGVPLAVALALTAFITGFFPLLGVWIVYIPICIYLLVFRGDATSAA
                     IYFGIGTSLTFFSSLFLRPMLAASQTRSFNFYWMLVALVSGVYAFGVPGVVLGPAILG
                     FAKAVMDTLVGEVRYETSLLKEEKVQKAEQEATDAAEAKSATAAD"
     gene            32494..33411
                     /locus_tag="VF240_18550"
     CDS             32494..33411
                     /locus_tag="VF240_18550"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX7177264.1"
                     /translation="MPDQNLFNVSAEVAEATAAGRAVVALESTVIAHGLPRPLNVETA
                     RRLERVVREGGAVPATVAVLGGRVCVGLDDAQLERVAFGEGVRKLSRRDLAAAVACGW
                     DGATTVATTLWAARRARIRVFATGGIGGVHRGALPDVSADLPELARTPLVVVCSGAKI
                     VLDLQATREWLETWGVAVVGYGCDEMPGFYTRRSGLAVDVRADTPRDVARLARARDAL
                     ELEGALVLTVPVPEEFEVDYELLKKTLADALDEAERAGVAGRELTPFLLSRMSEQSGG
                     ATLRANVALLEKNARVAAEVAGALASASC"
     gene            33550..34350
                     /locus_tag="VF240_18555"
     CDS             33550..34350
                     /locus_tag="VF240_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177265.1"
                     /translation="MKRRIFFAILLIAAAWFAGRHFTHRHQSAEAAGRDETHETFEMQ
                     PGARVEVRGINGNVKVETSATTTAEVHVVRTADNGSALANRRISVEQNSTGLVVRGES
                     RGGGWWRRIFGGSGQAREEVTLRVPRRVNLETRGINGQVVVGEIDGSVEVSGVNGSVE
                     LGQASVRAELNGVNGQVTLALDRAGERGIGMSGINGDVEFRIRESINADVEVTGQNGG
                     VTLNVPNVTRQERPNRSRMSARFGSGGAPVRLSGINGNVRFQTAAPTG"
BASE COUNT         5691 a        12106 c        11153 g         5517 t
ORIGIN      
        1 cgcttgaaga cgcggccgtt gtgccacgcg aagcggtagt agtaggggcc gtggagcggg
       61 ccgcgggcgc agcggcagtt ggccttgccg cagcgcttgt actgttggca gaccgcgccg
      121 gtgaacatcc cgtcgggcgg aatcttgggt aaaggttttc tcatgttatt tgcgctcacg
      181 cggcaggcgg gcccgcgaag gattcttttt tcctgatgcg gataaagcca agtgtggcga
      241 cctccactca cgcacgaaag ggccatctct cgggtgacga cggtgtcccc ggttccgttc
      301 gctggaacga ttcgggaacg attcttccct catcttcagt gttatactgc gcaccttgct
      361 tcacctgtga tggctgcaac cttggttaaa atctaagttc tcgcacgttc ccgcgcctta
      421 gctcatgact acagatcaga actgcctctt ctgcaaaatc gtcgcgggcg agattcccgc
      481 gcaaatcgtc cacacggacg accacacggt tgccttccgc gacatcaacc cccaagcgcc
      541 cacacacgtc ctcgtcatcc cgcgccggca catcgcctca ctcgaagagg cgacacaatc
      601 ggacgagagc gtgctcggcc gactgctcct cacggccgcg cgcgtcgccc gcgacgaggg
      661 gctggccgag ggcggctacc gcaccgtcat caacacgggc gcgggcgccg gccagtcagt
      721 ctttcacatc cacgcccacg tcatcggcgg ccgcccgctc gcgtggccgc cgggttgagc
      781 gaacgcgctg tgctctgctc gtggtgaatc gcgactttga aaacgcgaac ggaggccgac
      841 atgaagaacc tgatctgcgc gctcgtcacg ctcgccctcg tcttgctcgc ctcgccgcgc
      901 acgccgcggg cgcaagcagg gccgcaacag tcgagcggcg cttaccggat gttcctcggc
      961 gcgcaggaga ttggcaccga gacttacgtg atgagcgcgg agccggacgg cacgcgccgc
     1021 gcggaggcgg agctggtctt cggggtgaac aggtcgaagg cgacgaccat cgccgcgccg
     1081 aaccggcccg tgagcttcac ggtcgaggcg ggcggggcgc ggctgctcgc ggtggagttc
     1141 acggccgacg gggcgaaggt gacggccgcc ggccagacgc cgcgcgagtt gaagaccgcg
     1201 gccacggtga ttctggagaa cgccgtctgg catcactaca actacctgct cgcgcagtac
     1261 gacgccgggc gcgggggcgc gcagcagttc gtcgccttcc tcccgagtca ggccacgcag
     1321 ttcacactcc agttggagcg agtcggcacg cccacgttca cggtcggcgg ccggcaggtc
     1381 gccacagagc actaccgcgc gacgatgggt cagggtctct ccgtggagtt gtgggccgac
     1441 gcggcgcgcg tgccgctcct gatacgcgtc ccggcgcagg gcgtcagggt cgtccatgta
     1501 ggctccgagg cgctcgccga ggcgatcagc ccgtcggccc cggccgcgcc gcccgcgacg
     1561 gccgcgaacg aggcgtacac gagcgaggag gtgacgttta agaacggcga cgtgacgctc
     1621 gccggcacgc tgacgctgcc gcgccggggc gctgcgccac acccggcggc cgtcatcatc
     1681 accggctccg gcggtcagga tcgcgacggc tcgaacgtcc tcaacctcta ccggcgcatc
     1741 gccgaacaac tctccgcgag cggcgtcgcc gtcctgcgcg tggacgaccg cggcatgggc
     1801 aagagcgtga tgccgaagat gacctcagtc tcctatcgcg atctgattaa cgacacccgc
     1861 gccgccttcg actacgtcgc ggcccgcagg gagatcgacc cgaagcgcgt cgcgctcgtc
     1921 ggccacagcg agggcgcgca gacggctttg agcatcgcgg ccgaagacgg gcgcgtcggg
     1981 gccgtcgcgc tccttgccgg cgcttcgcgc cccgtcaacc acatcgtcta cgagcaggcg
     2041 ctctacatga tcgcgctcgg cgaggccatc gacccgtcgg acgccatgaa gctgccgcaa
     2101 atctcgcgtg acctgttgaa gattttcgat gacctgaaga cgcagcccgc gcccgcgcgc
     2161 cccgccgccg acaagtacgc gtggttccgc gagcacgcgg cgagcaaccc catggaactc
     2221 gcccggcgcg tgcgcgctcc cacgctcgtc cttaacggcg agcgcgattc acaggtgctc
     2281 ccataccacg cgctcgaact cgcgcaggcc ctcgccgcgg gcggcaacag gcaggtgcgc
     2341 ctccgcatct tcccgaacct cacgcacctc tttacgccct ccgcgctcga caagagcgtg
     2401 acgggcgagc ggtcggccga ggtgagcgag gagtttttaa agacgctcgc gacgtggctc
     2461 tcgggcgtgt tcggggcggg agggaggggg gaaagggtaa aggggtaaag ggaaaggggg
     2521 aagggcgagg cgcgtgcgtc gggtggccgg cacccgtctc ctttacccct tccccctttc
     2581 cctttacccc tctttggctc ttgcgccctc ccgcgccgcc gcaagctcca tgctgagcat
     2641 ttgtgccttc cgccgagaat cgttaaactc tacggtttga tgaacaccac tattcccggc
     2701 agtgaacaga tgagcacaga aaccctgacc ccgttcgagt catttctaaa cgagcacgac
     2761 gccgacgcct ggcaaggggc cgtcgccgaa ctgctcccgc acgtccacga ggttgaccgc
     2821 aacgccgtgc aaatctggtt cgccttcttc ccgctggcgc tcctgcgcgc cttccagcag
     2881 tcggaagacc ccgaacgcct cgcgggcgcc ttacagatgc agggccgctg gttgctcgcg
     2941 gatcagatag acacctcgca cacgttcctc tacggccacc gctactggcc cgaggtcaga
     3001 cgcgccgtcg ccgccctggc cgaagccggg gacggacaaa cggacgcgtc gctcgcgggc
     3061 cggattcggg agctggccgc gcgcgtcgcc tcggagttga aggtcgagca gtcgctcgtc
     3121 gtcggcatcg cggccgtcgc ctgcatgacg ttgcaacagg cgggactcga agcgttcaag
     3181 tcggcgccgg gcacagtcca catcgacaag aagtatttga agaagacgcc cgagcaagtc
     3241 ctgagcgcgc gcgcgcgcga cgaccggcag ggcctgttcg gcttcctgcg gacggaggac
     3301 aagctctgga cggtggtgtg ggacgagcac gatgatgagc gccgcttcaa ggcgatgcac
     3361 ttgcaggaag tcgcctcggg cgcggccgag gacaagcgtc cgtggcatga gttggacacg
     3421 cgatgcaccg ttgacgaggg gccgattccc gtccagtgcc gctcggcttc ctgcgggacg
     3481 tgctgggtgg gcgtgctcgg cggggcggag aagctgacgg aagtggccgc gcgcgagcgt
     3541 cgcaagatgc ccgagatggg ctacatcgac acggacgaag cgcgcccgct cattcgcctg
     3601 tcgtgtcagg cgcaggcgac gggcgcgctc tccatcgtca tcccgccctg gaacggcttc
     3661 ttcggcaagt atctcaagcg gcagcgcgag gccgccgcag tgggcgagac ggttcagacg
     3721 agcgaggcga cggcgtgagc gataggctga tgggcgaaga cactggggcg ggaggcgcgg
     3781 ggcaacatca ggagcaccgc ccgccgtcgc tccggcgcga cgccgagggg cgcgtcgtgc
     3841 cctccacact cgccgaactc atccagtggt tcctcgacta cgacgagcgc gtcgccgtcg
     3901 tccgcttccc cgccgtcgaa tctctcttcc agtggaagca gcaggaggcg ctcaaggccg
     3961 agccggacgc cttcgccttc aacacggcgg aagaccgcct cgccgtcggc gtcatgcagg
     4021 cgctccagac ctaccagacc gagcgcgcgc tccacgactg gctcaaggaa ctgctcggcg
     4081 cgctcgacga cgccaccaag accaacgagg ccatcctcga agcctacggc ctccactacg
     4141 gcgaggagca ttcagtcatc gaagaggccg agaagattcc cactcggcgc gagcgcgacg
     4201 tttatctgac ctgttgctgg ctggagacgc tatgcacggc cgaggcgcgc gtcctcggct
     4261 gggcctatca ggagctttac ggccggcctt ttcatccgca gaatttttaa atagaatcgc
     4321 ggcgcactgt ttataagggc cgccgacgtg aaccgaacag atggctaaag aaccgactaa
     4381 ttacaccaac acttcgcaga cggtcgtcgt cgatgccgac accttcgagt tcgagacgct
     4441 tgaagagaac aacggcgtcg ccaccgtcgt ccgcttccgg gtggagaacg aagacgtgcg
     4501 cgccggcgac gtgctcctcg tgctctccgg caccgacatc cacttccacg gcatgatcgg
     4561 ccgcatcgaa gaaggccacg gcaccgccac cgaccgccgc ggctccctcc tccccgccac
     4621 cgtgcagtaa aggcaaaagt gagaaagccg ggccgcgagc gtccacgagc cgccggaggc
     4681 ggcggaagaa tgtagcccac ggcgtgagcc gtggggacag tcacgagaaa gatttgaaag
     4741 ccccggaggg gcgaaagaac ctcaggccgc gttacgcgct ggttgaggtt ctttcgcccc
     4801 accggggctc cgttaattat gtcccgcgtt taccccggtc tcacgaccgg ggctacattc
     4861 tgccgccgct acgcggctca cggacgtctg cgatgacgtc gcgcggcttc ggcaatctca
     4921 cttttgcctt ttgccttatc aagtcgcggc cggctcttta cgcagcgtga agagcgtgcc
     4981 gccgccctcc gtgtcggggc gacgctgcca gcgcacttcg gcgtcgatca tcgaggcgcg
     5041 ggcgcggatg gaggcgaggc cgcgctcacc gcgcgacttc cggcggtcag tgtcgaagcc
     5101 gcggccgtcg tcttcgagcg tgaggtggaa gacgccttcg tccgtggccg agacgaagag
     5161 gcggacgcgg cgcgcggcgg cgtggcggca gacgttgctg acggcctcct gcacgatgcg
     5221 gtagacctgc atctgcacgc cgggcgcgaa gtgcaggcgc tcctcaaggt gctcgtcgca
     5281 cgagaactcg tagatgaact tgcagtcggg cggcatgtgc gcgacgcgct cggccaaggc
     5341 ccactccagc gcggcggcga accccacgtt ctccagcacc gacggactca ggtcttcgca
     5401 gatgcgtcga atctcctgcg agaccgactc gatctcggcg cgcaacaccg cggggtcgag
     5461 cgcgggcgct agtgtgcgtg ccggagcatc gggagcgtgc gcgccgccgc ctgagacttc
     5521 cgccgtcgcc atggcggcgg gcgcagtagt cgccggggcg ggcgcttcgg tcgcgccggc
     5581 ggggcgcatc tcgtcggcga ggagcatgag gcgtcggagg tctgcaagcg tctggtcgtg
     5641 gaggtcgcgg gcgatgcggc ggcgctcgct ctcggcctcg ttggctagtt gcaggcgagc
     5701 ggcggcgagc tggcgattgg cttccagtag gtcggagccg gcgcgcacga tcttgcggtg
     5761 ctggacgtag ccccagacga gcgcgacgag cgagagcgcg agcagcaccg agagcgccgt
     5821 gatcgtccac gggaacggcg cgcgctcgac gacgaactcg aagccgaagg gggcggacgc
     5881 gacgaggtcg atggtgaagg cgcgcgccgt gatgcggtag cgaccgggcc gcaagccctc
     5941 gacgcgaaac tgcgcgtcgt gcgcgagctt gcgggtgatg gttttgccag catcgtccgt
     6001 aaggaggaag gcgtattgga actgctcggg gaaggtgcgc gaggagttgg ccgccacgtc
     6061 gagcacgagg ccgttttgcg ggtaggcgag gtagaggccg cggcgctcgc cgtcgcgacc
     6121 cgcgccttct aagtcttcga gtgcgagcgg gcgcgcggcg acgacctgca cggggcggat
     6181 gacgggcggg gcggcgcccg gcccgtagag cgcgatgccg cgtgaggtgc cgacgacgag
     6241 cgtctgttcg tcgccgtctt cacgcgggag cgttgagctg agcagcgcga aggcgttctg
     6301 cgagggcaga ccctgctcgg cgtcgagcgc cgcggtgacg gggccgaact gttcgtcgag
     6361 cagaaccttg atgacgccgc cgccggccgt cgcgcaccac gcctgcggcg gcgcgggccg
     6421 tgaggcttcg gcggcggcgg cggcgtcgcc gcccacggcg gcgactgtct gcggcagcgc
     6481 ggcgaggacg gagcgcgcgt ccctgccggg cgcggcctcg acgagtgcgc cgccgggcgc
     6541 ctgtgcgtag aggccgcggt tggtggcgag ccagagccag ccgccggggc cgctgctcac
     6601 gccgtagacc gtgcttgcgc cgagcggttc atgggccggc tccgtcttca cgccgccctc
     6661 ggcgtcgagg tagaagacgc cgcccgtcgt cccgacccag agacgccccg cctgttcgtc
     6721 ggcgtggaga ctgatcgcct cctgaccccg cgcgttgtcc gcctcggagc ggggccagag
     6781 gatcgtgcgc cgcaactctt cgtcgaggcg ttcgacgccg cggccccacg tcgcgatgta
     6841 ggtcactccg ccgaggacgg cgacggcgcg gatcgtcccc gcgacgaccg gcggcgccac
     6901 gggccggctg acgggcttct ccggctcgac ccgttcaggg gcggccgcta ctttttgttc
     6961 gggctggccc tcggccgact gctgcgcggg cgtcgcagtg ggagcggcgg cgaccactgc
     7021 tccgtcggcg gcgaccgctc ccgcctgttc ggccttcgtc ttcgcgccgt cttccgcttc
     7081 gtcgccgccc tcgtctttct tctcttcagg tttgaaacgg atcgtgcccg gcgcggccga
     7141 cttgtccggc gcgagactcg cgtagaggcc gccggccgag gcgacgagca cgcggccgcg
     7201 ccggtcttcg gcgattgagt agacggtgcg gcggccgatc tcttcgaccg cgcgccacgc
     7261 gattccctca cgctcgcgca cgtagaggcc gcgattcgtg ccggcgagca agcgccccga
     7321 agaggttcgg tagagcgcgc ggacgaagtt gccgtcgagt tcttcggcga cgttctcgac
     7381 ccgcaggccg cgcgggtcgt agcggcagac accgcggtcg gtgccaaacc aggcgacgcc
     7441 ctcgcggtcg atgaagacgg agtagacgcg gtcggagcgg agcgccccgg ccgtcccggc
     7501 aaaggtgaag cgttcgattc ttcggccctc gcgcatgtgg aagacgccgc ggccgtcggt
     7561 gccgacgtag aggtcgccgc gcgcgtcgaa gcggagcgcg ttgaccgtgc ccgtcgcttc
     7621 gccggcgacc ttcgcggcgc gcgcgctcga cgcgtcggcc gtcgcggcgg cggcggcgtc
     7681 cgactcgtcc acgcggaaga ttccgctctc ggaggcgggg gcctgcgcgc ccgcgtggag
     7741 acggcctgcg ccgtcgcgct ccagcgcctc gaagaagaag gcgcgtggcc gcgacacgac
     7801 ctcgcgcacg gcgccgccgc gctcgacgga tagcaggccg cgcccacgcg tgccgacgag
     7861 gatggtctcg ccggccgcgg caaggctcgt cagttcgagc ggcgggcgag tggatgaagt
     7921 ggaagcggaa gaggacgtgc ccggcggcgg ctgcgcgggg aggatttgtt cggcgagcgg
     7981 ctcggcggtg taagtgccgt cgtctttccg gcgacagtcg aagacgaggc cgtctgaggt
     8041 ggcaacgacg gcgcgtccgc gcgtgggcgt gatgatggcg gcgacgcgct tgcccttcgt
     8101 ctcggggatt gacttgaact cgccggcgct cgtccgcagg taagcgccgt cgtcggtgcc
     8161 gacccagagc gcgccctctt cgtcggcgcg cagagcgagg acgcgcgcgg cggcgagtcc
     8221 cgcttcggtg acggtctgcg tgcgccggcc gtcgtagcgc gccagtcccg cgtcggtgcc
     8281 gaaccacaga tgcccttcgg cgtcctgcgc gatggcgcgc acgcggtctg acggcagacc
     8341 gtggaagagt gtgacggcgc cccagcggtg gaacgtcgcg gcctgcgcgg gcgactcctc
     8401 ggcgtcgcgc ttctgcgcta aggcgcagaa gaaggcaaca gtaaaaaggc aaaaggcaaa
     8461 agttacggag agaagcgcgt ggggtcgtgg tttggaaaga gttgtgcggg cgcttcccac
     8521 cttttgcctt tttacttttg ccttttgcct tctcacttgt gagtcttctg ctcttcggtg
     8581 aagcgcttgc ggtcgcgctc gatccaggcg tcgaggtcgg cggcggcctt ctccagttcg
     8641 tcggagttga tgaggccgcg gcgcagggcc tcgaagaagg cgcgtgtacg catgttgtag
     8701 gcgagcttgt cgtaggcttc gttgggcgtg ccctcgggcg tggagatgaa gagtttttcg
     8761 taaagcgtcg ccagccggct ctcgacgccg cgcagggaga gagagcactt gcgcgcgatg
     8821 gcccagttgg agagtccgag cgcgaggaag tagagcgcct cggcctcgaa ctcggtgagg
     8881 agcggctgct tgaaggagtc tttgaaggcc gggtcgatca tgtcggcgcc gtcttccaag
     8941 acggcggcga cgaagcgcag gaagcgcgac tcggggttgt tcttgtggat gaagccgtag
     9001 gcgggctgcg gctccagcga cttgatgatc ttgcgtatct cgttgatgta aacgtcgtgg
     9061 ccgaactgcg tccagaagat gatgcgcgcg ccgggaaact cacgccagat ggtgcgcgcc
     9121 gccttgacgc ccgacatccg cggcatctgg atgtcgaaca cgcacagctc gggccgctgc
     9181 gccagcgcca cgcggacggc ctcctcgccg tccgacgcct cgaccacggg cgcatacttc
     9241 gggaactcgc gctccagcag ctcgcgcagg tagtggcgct gcgccacgtt gtcttcggca
     9301 atcaggatgg acataagaaa ggttgtcagt agtcagtggc cagtagtcag ttgtctgtgt
     9361 gcgccatgcg ccgcgctaaa gataaaggct caagccgccc gtcgagaaag caactgacaa
     9421 ctgactactg acagttaaca cctgacctcc gcattttgaa tgtggatagc cacagacgca
     9481 aggggcgtgt cggcgcgcgg cggctgtaag gctactccgc cgcggggctg ttggccgccg
     9541 cattacgcgc ttattcaccg tggcggataa tcgcttcacg atagcgacgg tctatcgtga
     9601 acagtcggcg gcaagtctcc cgaacggccc ggcgcgtggg ttgtggcact tctcgggcgc
     9661 gggggccgca aaggggagaa gccgccgctc tccgagggcg cggggcgccg gggccgggta
     9721 gcgacagggt ttcggcgtcc cgcttaaaaa tttccgctcg cgaacacacc aaaaaccgag
     9781 gcgcgcgcct cacgcgcgcc caccgccttt ttcagttgag tgttagctac cttatgctca
     9841 acagaaatca gccttatagc tgcttcggcc ctgagtgtca gccggtcgcc gtcacttgcc
     9901 ctcgagcttc ttcagcgcct cgacggcgcc ggtgttctgc gggttcagct ccagcgactt
     9961 gcggtagttc tggatggcca gctccttctg cccgtcaacc atgtaggctt cgccgaggct
    10021 gtcgtaggcg ttgaagccct gcggatacat ctcgacgttg agcttgaaga tttcgatggc
    10081 ttcacggacg cgcttcgtct ggaggagttg atagccgagc tggttcaact gcggctcggc
    10141 gaagtcgaac tcggcggcgc gcgtcgcctt tagctcgcga tactgtttga cggccgccgt
    10201 tacgtccttc gccgtgatgg tttggaagag cacctcggag atgtcctcgc gggcgggctg
    10261 cgtcggcatg ccgtagagga cgcgggcgat gttctgcgcc acctgctgga gcttcgcgcc
    10321 ctgcgtgttg tctaagagca cgatgaggtg gcggctctcg gggatgcgga cgatgagtgt
    10381 ggcgaagccg ttgatgccgc ccgagtgctg gacgatggtc gtctccttgc cgccgagcgg
    10441 ggtcttctgg atgccccagc cgtaggcgta gttccgaagg cccggcgtgt acatcagctc
    10501 gcgcgtcttg gccgagagaa ccttgtcggt gtagagcgcc tgatcccaga gatagaggtc
    10561 ttcgatggtg gagtacatcg agccggcggc gtagggcagc gacatgtcga ggtagggcgc
    10621 gttgtcgtag ccggtgggcg tcttcgcgta gccggaggcg cgcttcgcca agagcgtgcc
    10681 gtggtgatcg tagccggtgt tcttcatgcc gacggggtcg aggatgcgtt ccttcaagac
    10741 ctgctcgtag gtcttgcccg tcacctgctc gatgagcgcg ccgaggagga agtagccgga
    10801 gttgttgtag gcgaacttcg agccgggctc gaattcgagg tcgccgctgg cgaacttctt
    10861 gacgaagtcg gagacggcgt aggggtcgcg gctctcctcc ttgaggaagt tcgcccggcc
    10921 cgtgtagctc gggacgccgg aggtgtggtt gagcagctgg tggatggtga tgcgcccgcc
    10981 cgtgtctttg cggtagtcgg gcaggtagtc cgaaagcttg ccgtcgagct tgatcttgcc
    11041 ctgctcgacg agctggagga cgagcgccga ggtgaactgc ttcgtgatgg agccgagccg
    11101 aaacttcgtg tccggctcga cggggatggc ccactccatg ttggccatcc cgtagccctt
    11161 gcggaagatc acgcgccctt gctcggccac gagcaccgcg ccgttgaact gccggatgcc
    11221 gtggtaggac ttcaccaact cgtcaatatt cgcggccttg tcggccgccg ccgccggggc
    11281 ctgggcctgc gcggccgtcg cgcacagcaa cagggcgaac gccagaacgg cggacgaagc
    11341 gaaacgcttt ctcataattt aatttctccg tggttagagg gtttatagcg atgccagatg
    11401 actctgtacg cgccgccggc cgacggggtt gcatcagaag cagtgataag tgatgagtga
    11461 taagtgataa gaagcaatta gctcttagct cttagctaac agcctacagc caacagctaa
    11521 gaactaacag cttccgttct ttctcatcac tcatcactca tcacttatca cgtcttcaaa
    11581 tcatccacgt ctcgcgccca cggtagtcgg tgaggtgtcg gtcggggtgg cggcgcttga
    11641 actccttgta gcgggcgagg agtttttggt agcgggcgtc ggaggagctt aagtgctggt
    11701 ggggaatctg ctgaagatgg cgctcgacct tccagacgtt gcggggtgag aagcgccagt
    11761 cgtagccgcc gaggtcgcgc aggtcggcga ggccgtagcc ggtgatgcgg cccgtgtgat
    11821 cgacgtaggg gtcgaagtag ctccacacga gctgacgcag attcgggaag acgggccgtc
    11881 gcccgtgcag gcccagatcg cgcgagcgcg caacggcgcc ccacagtccc ttttgtcgga
    11941 agacgaagag gacgtggtcg agcaggtctt gcgactcgaa actgaggagc agcggcgggt
    12001 ggccgtgctg ttcgaggatg acggccgcgg cgagcgccgc ctccagacag tgggcctcgc
    12061 cccgccggac gacctcgcgg aacgagcgca gcgtgccgcc ctcgcgctcc cagttgtagg
    12121 gcagacgcga caggtaacgc tgcacgcgcg caggcgtgcg gtggagcgcg acgatctccc
    12181 actcccgcgc cgtgaactgt tcccactccg gtttgccgct catcatcgtc cgaagctaat
    12241 agcagaggcc ggcgcggaaa ggcaaaagcc ggcgaggacg aaagttgaag aagggcgcgc
    12301 gctcatacgc ccgcacgggt ggcgcgtttg tcggcgggcg tcaatgatga taaagtgctt
    12361 cttcaagaga aacgccgccc aacatcttga aaaattcacg ctccccgacg cgaaaacgtc
    12421 ctaaacggcc gaccgcgcgt caaggggttg aggcggggcg cccgacccgg ttcgggcgcg
    12481 gccattgatg tgggagatgc gatgtctccc gaatttatat ttgaagtttc ggaaaggcgc
    12541 cgagcggccg ccccccgacg cggggcgcgg ccgcgcactt ccgagccgag gcagcgggcc
    12601 ggttagagaa taagttttca cccccggccc gcgggttata tttcgtgagc agaagtcaaa
    12661 catcatcctc cggcccggtg cgctcaccgg ccgagctgga aacgcgctcc gccgccgagc
    12721 gtctgagcgc acagattgag gacgcgctcg ccgccgtggc cggccactcc aacgacagca
    12781 cggaggagtt ggaagcgtgc gccgaccgtc tggagcgggc cgcgcgcgac ctcgccgccg
    12841 ccctgcgcga gctggccagt cgtcgcgccg gggccgcgga ggaatagccc ggcggcgttc
    12901 ggcaagcatt acaaaaaaag caggagcgcc ggggctgtta tttaaagtcc cccgcgctcc
    12961 tcgcgtgcat tcgggtggca tttgttcgcc cggccaacaa agaaatcaac tgccgtgaat
    13021 cgtcaatcgt gaatcgtcaa acgattcagt tgaagccaac ccgagttgcg attcaccgcc
    13081 cgagtcgaag ttcacccgac cacgattgac gattgacgat tcacgaggag aatcctgatg
    13141 gtagcaacca gacagcaggc ggcgcgcgaa ctggggcgca ggcgaagcgc cgtgggtctc
    13201 gacgcatacg gcatccagcc ggcctacgac gtttactgga acctgacgcc gccggaactc
    13261 tacgaggagg tggcgcgccg cggcgagggc cgcatctcgc agcacggcgc actgctcgtg
    13321 gacacgggcg agcacacggg acgggccgcc aaggacaagg ccatcgtgcg cgagccgggg
    13381 agcgaggaca aagttttctg gggcgacgtg aacaaggaat tccccgccga gcggtttaac
    13441 gccgtgcgcg accgcatgat gcagtacgcc tcttcccgtg aactcttcgt gcaggacacc
    13501 ttcgccgggg ccgacccgcg ctaccgcctg cccgtgcgcg tcatcaacga gctggcgtgg
    13561 cactctctgt ttgcgcgcac catgttcatc agccccgagg agtcggcgcg cgaggatcac
    13621 acccctgagt tcaccatcat caacctcccg agcttcaagg ccgaccccgc gaccgacggc
    13681 acgcgctccg acacgttcat cctcatggac ttcagccagc gcctcgtcct catcggcgga
    13741 accagttacg ccggcgagac gaagaagagc gtcttcacca tcctcaacta cctgctcccg
    13801 cagcgcggcg tgatgtcgat gcactgctcg gcgaacgtcg gccaacaggg cgacgtggcc
    13861 atcttcttcg gactctccgg cacgggcaag accactttga gcgccgaccc cgagcgccgt
    13921 ctcatcggcg acgacgagca cggctggtcg gacgacggcg tcttcaactt cgagggcggc
    13981 tgctacgcca aagtcatcaa gctctcggcg gaggccgagc cggacatcta ccgcaccacg
    14041 cgtatgttcg gcaccgtcat ggagaacgtc gtctatgatc ccgtgacgcg cgtccctgac
    14101 ctcgacgact cgtccaagac cgagaacacg cgcgccgcct acccgctcac ctccatcccg
    14161 aacattgtga cggaaggtca cgccggtcac ccgtcgaaca tcatcatgct gacggccgac
    14221 gccttcggcg tgctgccgcc cgtcgcccgt ctcacccctg agcaggcgat gtaccacttc
    14281 ctctccggct acacggcgaa ggtggccggc acggagcgcg gcgtcaccga gccgcaggcg
    14341 acgttttcga cctgcttcgg cgcgcccttc atggtgttgc accccggtgt ctacgctgac
    14401 ctcttaggcc gtaagattca ggagcacaag gccgcctgct ggctcgtcaa cacaggttgg
    14461 tcgggcggcc cttacggcga aggcaagcgc atgtcgatca agcacacgcg tgcgatgatc
    14521 cgagccatcc tctcgggcac gctcgccgca gttgagacac ggcctgaccc cgtcttcggc
    14581 gtcggggtgc ccctctcctg tcccgacgtg cccgccgagg tgctcacccc gcgcaacaca
    14641 tggaaggaca aggacgccta cgacgccaaa gcccacgacc tcgcccgccg cttcaacgac
    14701 aacttcaaga agtatgaggc cggcgtctcc gccgaggtgc gcgccgtcgc cccgaaggcg
    14761 ggctgaagta aggcaaaagt agaaaggcaa aaggcaaaag tgaggaagcg tctcgcctcg
    14821 tcacttttgc cttcctccgc gcccccttcg cgccctgcgg cgtctctgcg gtgaatccga
    14881 cccgccgcac actcaactca aaagctatct agtccttgcg cttagtcttc gtctctcccc
    14941 gcgcgctcat aatttcctcg acaatcttga ccgcgagcat cggcgccgcc gcgttgccgt
    15001 cgaaggccgt gaccttgccg gtggcgagaa cgacgcccgt gccgcgatgg gtgacgcggt
    15061 aggtgaaggt gtaagtgaag accgggcggt ctacttcgat gaccacgtcg gcgacctcgc
    15121 gcttttgcca gccgtcaaca atcgccatct gccacgcgtc catctccgcg cgcttgctca
    15181 aggcgttttg cagttgcacc ggctcgaaga acgacgtgcc ggattcaatg tagatcgttc
    15241 gcgcgcggcg cagcagggcc ttcggactag cctcggcgat cttcgcggac tcctctgccg
    15301 ccagcttctc ctcggccgcc ttccgcagcg ccttctcctt gaactcttcg gccacgcgct
    15361 cctccgcgct ctgcgcgggc cgcacggtga cggtcacgtg ctgcgtctcg gcctgctgcg
    15421 cgtgcgcgcc gacgccggcg ataaaaattg ccgccgtgaa aagtatgcct cggatgatgc
    15481 cgttcgtgtt caccttcgcc tccttcgtaa aagtctctaa ctccgtctga cgtggaaacg
    15541 cgttcgcctt ccgccctatg aatcgctcca ctcgtcgtcc tcttcgtcgg ccgccttctt
    15601 cttccggcgc ggcgacgtgt tgtcggacga ggcgcgcacc tgtcgcatct tcttgatgat
    15661 ttcagtgccg aggtattcgg cggcgagttg cttgttgatg gcgaccacct tgccgcccac
    15721 gacgacggcg ctcgtgcgcg gatcgacgac gctgaagatg tagttgagca tcgtcttgtc
    15781 gatcttgatg acgaggtcgg ccgcccgctc ttcttcgacg acaacgaagc cccaatcgcg
    15841 aaactccgcg tgcttcagga gcttgtattc gaggtacttt gcatcgacgt gccggcccgg
    15901 ccggacgtgg attttgcgcg cggcgcgcag ggcggcgacc ggactcgcgt cctccgccgt
    15961 ctcccgcgcc tgtcgcgcgc gactctgcga cggcggtaac tgcgtctgcc gccccgtggt
    16021 ctgcgccgcg agcacggaga ggcccgcgag cgcgaagatg agcacggccg ccagccagcc
    16081 gaaaacttcc acaactctct tcataatcaa tctcctcctg acagctctct cagaaacgtc
    16141 ttgcatgtga tacctgctcc cgccgcacaa gccagtgcaa cgcccgtgcc gccgcgcccg
    16201 gcggcgtcaa acgcgcccgc gcggcccgtg cgccatcacg aacgcgccgg agtctgtcgt
    16261 caggtgaaag aatattgcgg cgaaggagaa gggaagtcag gagtcagaag tcaggagaca
    16321 gaatcggcaa cagtaccgcg agcggaagcc tcgcgggtct cgcgtgtgca gtgcgggctc
    16381 ctcattgtac gcacacccgc tcgctaccgc tcgcggtact gtactcctga ctcctgactc
    16441 ccttgtcctt tcgcgacttt tgaggtcgat tctggcaaga tgaaggggat atggctgaga
    16501 cttccgagga ttcattgagg gcagagggcg cgcgctcctc cgcggtaggc gcgggcgtgg
    16561 ggcggcccga cggggcggcg acgctgcgga cggtggtcga gatggtgcgc cgcgtgatgc
    16621 gcgccgacac ggccggcgtg gccagcttct cgctcgacga gcggacgatc acgtggaagg
    16681 cgatgagcgg cttccgctcc gtcgcggccg agggcgagcg cgagatcgtc acgccgttgc
    16741 gcggggagtt cgccgagcgc gcggccgccg tcggcgagtc gatcatcgag gtgcggggac
    16801 tcgccggcga tttgcccgtg agcgagttcc cgctgcacag cgccgagggc gtgcgcgatc
    16861 tggcgctcgt gccgctctcc gcgcgcggcg agacgctcgg ggttctcgcc gtgggctttc
    16921 gcacctcgca ccgcttcgcc cccgaagaga agcaactgct cgaagacctc gccgagatgg
    16981 ccgccctcgc cttagacaac gcgcgtctat ttgagaccat cgccgccgcc aagaaaatct
    17041 gggagcagac cttcgacgcc atccctgacg gcatcatcgt ccacgacgag cagatgcgcg
    17101 tcacgcgctg caacctcgcc gccgccgaga tgatgggctt ctccgcgccg gccgaggcca
    17161 tcggcatgtc ggtagcctcc gccttcgcac gactcttcgg cgagcgcgcc gccgcctacc
    17221 acatgcggca gggagcggtg gcccgcacgg cgagcgcctt cgagttgcag gccgaggacg
    17281 gccgccgtta tctcgtctcc gtctccccgc tgatgagcat ggagacggcc ggctgggaaa
    17341 gcgaccccga cacgaccggg acgacggagc ctgccccgcg cacgacgtgg agcgtcatca
    17401 gttggagcga catcaccgag cttgccgaga tacaggagca gctcgcgcgc tcgcgccgtc
    17461 tggccacggt cggacagctc gccgccggtg tggcgcacga gatcaacaac ccgcttgccg
    17521 ccatcgccgc ctgcgccgag gccgtcgtgc gcgacgtgcg cgcaagcgag ccggcgtcgg
    17581 cgctcgccgc cgagggcaac tggaacttct acctcgaaga gatcgtccga caggcgttgc
    17641 gcggcaaggc catcacgcgg ggcctgctcg atctttcgcg ccagcggcgc gcgaagcgcg
    17701 aggccgtgga gctgaaccag ctcgtcgagc gcgcggcccg cctcttcgag ccgcgcgggg
    17761 cggagcgcgg cgtccgcgtc gaactcgacg tggacgcgcg cgtgggcgag gtggcgaccg
    17821 acgaggggat ggtgcgccag attctcgaca acctgctctc aaacgcgctc gacgcggcgg
    17881 gcgcaggcgg gcgcgtcggc gtctcgaccg tgctcgacgc ggcgcgcgtg cgtgtcgagg
    17941 tcgaagactc cggccccggc atcccgcccg aaatcctcgc gcgcgtcttc gaccccttct
    18001 tcaccaccaa agaccccggc cacggctccg gcctcggcct cgccatctcg ctctcgctcg
    18061 ccgaggccat cggcggcgca ctcaccgtcg aatcgaagcc cggcgccggc acgcgcttcc
    18121 gcctctggat tccccggcgc tcgccggaga gcaacaagac gcagtgacgg gtgacgggtg
    18181 atgagtgaca agtgatgagc gacaagtgat aagatgttcc cccgacgctg cgctctcatc
    18241 atcctgtccc tcgacgcttg tcgctgccct tatgtctcga aaaatccgtg tgctcgtggt
    18301 tgacgatgac gccgtgctgc ggcgcgtcgt gtcggagcag atcgcgaagg ccggcttcga
    18361 cgcgcgcgcg gccggctcgg gcgaggaggc gctcaaactc ctcggcgacg cggacctcga
    18421 cgtagtgctc ctcgatatcc gcatgcccgg cctctccggc ctcgacgccc tgcgcgagat
    18481 acgcaagctc gacgacccgc ccgaagtcat catgctcacg gccgacacct cgctcggcac
    18541 cggcatcgag gccatgcgcc acggcgccta cgactacctg accaagccct ccaccttaga
    18601 cgagatcgag gccgtcatcc gcaaggccga cgagaagcgc cggctcgtgc gccagaacgc
    18661 cggcctgcgc gaggccgtgc gcgcgcgtgg cggaggtggg ggcggcaaca gcgacgacgc
    18721 gcccgcgctc gtcttcaaca gccgcgcgat gggcgacctt gtggcgctcg ccgagacggc
    18781 cgcgcgttct gattcgaccg tcatcatcac gggcgagtcg ggcacaggca aggacgtgtt
    18841 cgcgcgcttc atccacgccc gcagcgcgcg ctcggcgcag cccgtcatca ccgtcaactg
    18901 cgcggccctg cccgaggcgc tcttcgagtc ggagttcttc ggacacgagc gcggagcgtt
    18961 caccggcgcg ggcgcggcca agcccggact catcgaggcc gccgactcct ccacactctt
    19021 cctcgacgag gtcggcgaga cggcgccggc cacgcaggcc aagctcctcc agttcctcga
    19081 agagggtcgc ttccgccgcg tcggctccac gcgcgaccgc gcggccgacg ttcgcatcat
    19141 cgccgccacc aaccgccgcc tcgccgaaga ggtcgcggcc gggcgtttcc gctccgacct
    19201 cttctatcgc ctcaacgtca tctcgctcca cgtcacccca ttacgcgagc gcgccgaaga
    19261 cgtgcccgcc ctcgtcgagc acttcctcgc gcgcaaccgc gaacgcttca atcgacccgc
    19321 gctcgacctc tccgacgaag cgcgccgccg ccttgagtct tacgactggc ccggcaacgt
    19381 ccgcgagcta cgcaacctct tagagcgcgc cgccgccctc gccaccggcg accgcatcga
    19441 agctgagcaa atcctgcccg cccgcgacaa cagcaccgac accgccgctg ctaacgctaa
    19501 cgccaacact tcccccgaca cctcgacctc cgcgcaacaa ccgcccgcaa ccctcgaaga
    19561 actcgaacgc cagcacatcc tccgcgtcct ccacgagacg ggcggcaacc gcgagcgcgc
    19621 ctccgccgtc ctcggcatct ccgcgcgcac cctctaccgc aagctgcgcg aatacgagac
    19681 cgatgccgcg cccggcgccc tggctactga agattaaaac gattagaagg tcgcttcgac
    19741 gacctgcttg atttcgcggg gtagtgtgag gggggcgaat cgctcgccgc tgtagagata
    19801 atcttcgcct gactcgtcca cgacgcgcag ccacccttcc gggtcattcg gctcgggcgg
    19861 aagaatttga tagagcttac gcaactcaag cgaggcttca tagccctcgt tgttgacgca
    19921 cagcgcgtat gccgcatttc gctttcgacg tgtcatggct caatccagaa gacgtttaac
    19981 tttcatctct ttcctgccga tgccgtgggc ctcgtaccag tgaagttcgg cccggtgaac
    20041 ctccccgtca gtcaactcca ccgacgccag ccctctcacc ttgcgccagc ggcccttccc
    20101 gtacctgcgc cgcaggcgct cgacttcccg aatcttacca ccgacggcga tgttttcaac
    20161 gaccgtgacg gggccgatga tgcgtcgaaa ttctagcatc gagcgtcgct caagagcgcc
    20221 gggcgcgcaa ggtaattcaa aagctgagtc atcatatcac cccctcacgc gtgtagccca
    20281 tctaccgaaa attgtaacgc cggcggctga caaaacgtca gtcgccggac attttgtcag
    20341 ccgcgattgt cagacccgcc gcccgcgcgc cccgcgaccg gccgcgcagt gatcctccta
    20401 aaaccaaata ttgtttcact tgcagccctg tggcgcgccc cggcgcgggt ggcatggggc
    20461 ttgtgatgcg ttcgtgcggc ggacgtgcgt ggcgcggctc gccaatctct gccaaaggta
    20521 aacgaacaaa tggcccgtgt gatgatttgc gacgacgacc gagcgatggc ggcgaagctt
    20581 gtggccgctt tgcgcgcggc cgggcacgag gccgacacgt gccagcacac gatggacgtg
    20641 ttgcgggcgg cggccggcgg gcgcttcgac ttaatcgccg tcgggctgga catggcgggc
    20701 ttcggccgcg ccggggccgt cgaggccctg cgcgagatcg cgcccaacac ttcgctcatc
    20761 gccctgcacg accgccccgc cgagatcatg cgcgcggcga cgctcgctcg cgtcgcggcc
    20821 gtgctcccgc gccccgtgcg cgtcaaggat ttcatgtacg ccgtcgcctg cgcgctcaac
    20881 gagcagcaga gcgtgatggc cgatcccgtc tgatgcgtgc agcgccggcc ggaagaggct
    20941 ccggcgccgc acgactgaaa ctctccggac gccgcacgcc ggagcctgct aaaagcctca
    21001 ccccaccgaa cagtcaaacc cggaaggaga cgaagatgaa caaccctgtg agaaagatcg
    21061 gaacgcgcac catgctcgcg ctctgcgtcg tcgccctgtg cggcgtcgcc gccatgagct
    21121 tcgcgcgggg ccgaacggct gcggccggcc gccccgaggt caaggtcgcg ctcgccggca
    21181 cggtcgagcg tgagggcaaa caggtcgccc tcgcggcggc cgagtcggtc aagcccggcg
    21241 aagtcctcaa ctggcagatc gccacggtca acgagggcac cggcgccgcg cgcgagtaca
    21301 agaccgtcgg cgaaatcccc gccggcacgg cctacgtcgc cggcagcgcc gccggcgaga
    21361 acggctcgac cgtcacctac tccatcgacg gcggcaagac cttctcgacg cagcccatca
    21421 tcgagcagaa gcagccggac ggcacagtca agcgcgtcgc ggcgcccgtc tcgatgtaca
    21481 cgcaggtgcg ctacgagtgg gcggacgagt tggccgccgg cggccgcgtc aacgcctcct
    21541 acaaggtgcg cgtcaagtaa acggcggccg ctcggccgcg agagtctcct ttcaagctca
    21601 cgccgaagcc acacactggc tcgccagccc gccgacccaa gacttcaaga ggaacgacga
    21661 tgaaccacat acttcacacc aggctccccc acggccgcgc actgttcgcg gtcctcgcgc
    21721 tgctggtgct cgccctcggc gcgccgccgg cgcgcgcgca gcagaccacc ggcggcaccg
    21781 tcatcagcaa ccaggcttcg gccagctact ccgacggcac caacagctac tcggtcaact
    21841 ccaataccgt gaccgtcacg gtcgccaacg tcgcgagcct gaccatcacg cccgacggcg
    21901 gctcggtgcc gacggtcgtc gcgggtcaga ccggcgttga cttcaccttt accgtcacca
    21961 actcgggcaa cttcgccacg caggttcgct tccccgcgag cggcgcggcc gtcgtcccct
    22021 ccgccggtct caccgtcacg caggccgtca tcgacgtcaa cggcaacggc atcgacggcg
    22081 gcgacatcga catcctcggc aacggctcgg ccgtgctcta cccgtcggct tcgccgtacc
    22141 tcgcgcgcaa cgcctcgttc aacgtcgtcg tccgcacgac cgttgacgcg gccgccgccg
    22201 cgggcgcgac cgtcggcgtc acgctcggcg acgcctcggg cgacaacgtc gcgtcgaacg
    22261 gctcggccgc cgaggtacgc acctccgtcc cgtcgggtgt cacgctgacg ggcagcgaga
    22321 gcgaggccat cggcagcatc acgaccaccg tccagaacga cgcgcagctc cgcctcggcc
    22381 tctcggcccc ggccggcccc gtcgcgctcg gctcgaacgt cacctacacg tggacgctcg
    22441 acaacgtggg cgcgcgcgcc gtcgcgccgc gcacgctgac gaacgcgccg gccggctcgc
    22501 agacaggcgt cttcgtcatc gcgccggttc ccagccgcac caccttctcg tcggtgacgc
    22561 cgcccgccgg cgtgaccgtc ctctactcga cctcggccct gacgaccgac cccgtgacgg
    22621 gcgccgtctg gacgaacgtc gcgccgccgc ccggctcggt cacgcgcgtc ctcttcaacg
    22681 tcggcgcggc gctcgcgccc gccgcctccg tcgcgaacat gcaaatggtc gtgacggtca
    22741 acacgggcat caacgccacg ctgccgctct acgagatcgg cgacgccttc ggccgcaact
    22801 cggtcaacgc cttgctcacc gaccagtcgg gcgacctcgt agtcaacaag ggcgacggca
    22861 acgccgactt caacgagccg cgcctcggcg ttgacccgtt gagcgcgacg cagggcttcc
    22921 agctcccgac gcttttgacg caggtcggct ccgtcctctt cgggccgtcg ggcgcgcccg
    22981 ccgcggtcgg cccgaccgac aacaacgacg actacaccaa taggacggtg gccccggcgg
    23041 ccatcgccgg gctgtcgcac ctcgacacgc tctcctcggc cacgaccgtt gacttcacca
    23101 acacggtgca gaacacgggc aactccgacg acacgttcac cttcacggcg cccacggcgc
    23161 ccgcgggctt caccgtccag atcagcacga acggcggcac gtcgttcacc acgctctcgg
    23221 gcggcgggag cacgacactc gccgtcgcgt tcggcgcgtc ggccaacgtc atcgtgcgcg
    23281 tcaccgcgcc cgccggcacg gccatcctcc agacgggcgg cttcaacacg gtggtgcgcg
    23341 cgacctcggg cacgacaccc gcctcgtcga acgacacgca cgacaactac tacacgggat
    23401 tcctgaagct cgtgaagacg gtgctggtct tcaactcgac cggcatcggc ggagcctccg
    23461 accccgtccc cggcgcggac atcgagtact cgatcactta ctcgaacgtc tcggtgggcg
    23521 gcggcggcgc gggctgcgtc gatctggtcg cgtcgagcgt cgtcatcagc gaggacggca
    23581 acgccgcgcc caacaactgg ggcacgacga ccacgcagct cctcgtcccc gcgccgtccg
    23641 actccaacgg cggcgccatc accgacggca acaccaacgg cgccgtcacc gtggccacga
    23701 cgttcctcag agacgccgcc ggcacggtca accccggcgc ctcctatgtg ttcgtcttcc
    23761 gccgccggat caactaagcc gcgggtaagg ggcaaaggga aagggggaag ggcaccgcgc
    23821 atcttccccc tgacccttta ccccttaccc ctttcccctt actcgccctt cgttcctctt
    23881 aacctccatg tcccttcgcc gccccagacg gatcgcccgc gcgctcgcca tcacgctcat
    23941 cgcggcgtcg gtgatgggcg cgcacgcgcg tccggcgcgc cgcgacggcg ccgacaccgg
    24001 caccgtcatc tccaaccgcg ccgaggccgt ctaccacgac gcggacggcg ctagcttccg
    24061 caccgtctcg cccaccgtct ccgtgacggt cttggccgtc tccggcctcg tcgtcacgcc
    24121 ggacgaaacg gaagcctcgg cggccgtctc acccaacgag cgtgtcaccc gcctcttccg
    24181 catctgcaac accggcaaca cgcccgacat ctacaccctc acgcgcgccg acgtgagcgc
    24241 ccccgccacc ctccacacgc tccacttcga cgtggacgcc acgggcacgc tcacggacgg
    24301 cgaccgcgcc gttcaactca acacgacgat gagtccgcgt cttgcgcgcg gcgagtgcac
    24361 ggggctgctc gccgtcgtcg atacgaacgc gagccagccg caatcacaaa tcaatatttc
    24421 catcatcgcg cgctccaacg tccttggcac cgtcaacggc gccgtcgaag atacggggac
    24481 gatcatcaac gccgtcggca acggcgcgcg cctcagctcg cccgacgacg cgcagctccc
    24541 gccctcgaaa ctcgtcgagg gccacgaccg catcacggcc gcgcacgccc agacgctcaa
    24601 ctatctcgtc tccttccgca acagcggcga cgtgaccgcc cgccgcgtcc tgctgcgcga
    24661 cgacctgccc gaaggtctcg actacgtcgc cggctcgctc cgcctcggca caagaaatct
    24721 gaccgacgcc gaagacgccg acgagggact cttccgcgac cgccacttcg agattcgtct
    24781 gcccgaagtg aagatcgacg agacggtgcg cgtcgccttc caggcgcgcg tcaccacgcg
    24841 cgtcgcgccg ggcgagggcg tcgtcaacac ggcgcaaatc tcggccgaca acgccgcgct
    24901 cgtcgcctcc tccacggcga cggccgtcgt caatcccttc ggcctcgtct acgagggccg
    24961 cggcggcgcc gcgagcgtca tccccggcgc gcgcgtcgcc ctgctcgccg actcgacgac
    25021 cggcgcgacc gtctcgcttc ccacgggcgg cgccgtgccg aacgagtcga acaccaaccc
    25081 ttatctctcc gacggtcagg gccgctggag tttcgccctc tcacccgcgc agctcggggc
    25141 cgcggactcc gcggcgcgct acttcctcaa cgtcaccgcg cagggcttcc gctcccgcat
    25201 gatcgaggcg accgtcacgc cgcaacccgg ctccggcgga ctcttcacgc ttctggtgcg
    25261 cgcgctcgac ggccagcccg tcgcgcaagc cggcggcttc gagctgacga ccgaagacgt
    25321 gtcgattccg cgtctcgccg ccttcgcctt aaacgtgccg atgttcgaga actcggccat
    25381 cgagattcag aagaccgccg accggcccgc ggccgaagtc ggcgaggtcg tcacctaccg
    25441 cgtcgaggtg cgtaacgcga ccgactcggc cgtctccgac acggtggtca acgacacgct
    25501 cccgccctcc ttccacttcg cacagggaag cgcccgtctt gacttgccgc ccgcggggcc
    25561 gcgccccatc gagcctgaag tggcgtcggg cggcctgctc atcttccgtc tggggacgct
    25621 gcccagcggc gcccgcgcca cgctcgtcta ccgcgtccgc atcggcgcca acgcctccga
    25681 aggcgagcag ttcaactcgg cctcggccgt cggccgtgtc gccatcacgg gcgagcgcct
    25741 gacgacggag ccggcgcgcg cccccgtgcg cgtccgccgc ggcatcttct ccacgcagca
    25801 gatcatcatc ggccgcgtct acgaggacgc gaacgccaac ggcgctttcg actcgggcga
    25861 gcgcgcgctc gccggcgtcc gtctctatct caacaacggg cagtcggtca tcaccgactc
    25921 ggccggtcag tacaacttcc ccacggtcgg cgaaggctca cacgtcatct cgctcgaccc
    25981 gctgacgctg ccgcccggct acgccatctc gaacgccaac cgccgcgagc agacctcgtg
    26041 gacgcgcctg ctccgcacgc ccctcggcgg cggcgcgatg ctccgacaga actacgcgct
    26101 gctgccgccc gccgactccg ccgcaacaac cacgcaagcc gcccctcgct ccgcgtccgc
    26161 cggcgcgtct gttgacgcaa ccgcgtccgc ctctttgaac tacgcgcaga gcggagccgc
    26221 cccgcgccgt ggcgacggcc agcagagcgc cgacaagagt gccgctggtg agtcgtcgaa
    26281 cggcgcgaac ggcgctacgg ccgcggccga gacttcgcac gcttccaacg gcacagaggc
    26341 cgcgcgcgcg cccggaactt tcgaggtcgc cacgacggag acggtcgagc cggtcgcgcc
    26401 cggcaaggtg aaagtgttgt cgccggcgcc cggcgtggtc gtcgctggcg cggcgctcga
    26461 aatcgaagcg cgcgtggcga atgactggaa ggtgtcggtc gaagtcgaag gccagagtgt
    26521 ttccgaggcg aagatcggcg agcgccgcgt ggatcacaag aacaaggtga cgacctacgg
    26581 cttcgtcggc gtgagcgttc ggcccggccc caatcgcgtg acggtcacgg ccatcggccc
    26641 cgacggccgg gccggcgaga ccgtcgagct gacggcctac ggccgcggcc ccgcgaagcg
    26701 tctggaaatc gtcagcgaca aggccgagct gagcgcgggc gggcgcgacc gcacgaccct
    26761 gcgcgtgcgc gccttcgacc agtggggcca ccccgcggcc gacggcgccg tcgccctcga
    26821 agtctcagcc ggccgtctgc tccgcgtcgc gccggctgaa ggcagcaagg gaacttctga
    26881 ggaagcccgc cgcgcggccg aaggcgtcgg gagcatcgcg gccggccatg aagcttctac
    26941 ggaagcgcag gcggcggccg aagcgaagtc gcagatcatc acgctttcgg gcggtgaggc
    27001 cgtggcggaa ctcgtcgccg acggcgcgcc gggcatggtc gaagtacacg ccaccacggg
    27061 cgcgctcgcg gccgacgcgc gcgtgcgcgt cacgcccgaa atcaggccca cgatcctcgt
    27121 cggcctcgcc gaagtctccg tcggcagcgc cgccccggaa atctccatgc acggctcgca
    27181 ggagcgcgcg cgcagccgca tcgccttctt ctatcgcggc cccgtcttcg gctcgaagaa
    27241 cctgctcacg ctcgcctacg actcgcagcg gcccctgaat cgcacctcgg gcagcgaccg
    27301 catgttccag ctcgacccgc tggagcgcgc ctacccgctc ttcggcgact cctccacccg
    27361 ctacgaagac gcgcaatcga actccaagct ctacgcgcgt ctcgaccgtg gccgctccta
    27421 cgtgatgttc ggcgacttcg acaccgagaa ccgcgacgcg ggtctggcct cctactcgcg
    27481 ccgcctgacg ggcgtcaagc tccacgtcga aaactcgaag ggcgactttg tctccgtgac
    27541 gggcgcgcgc cccgacaccg cgtttgcccg cgacgtgttc cccggcggcg cgctcggctt
    27601 cgtgcgtctg acgcatgcgg acgtgatgcc cggctccgag gtcgtcgcca tcgaagtgag
    27661 agaccggcgc aaccccgagg tcatcatctc gcgcgagccg ctggtgcgct cggtcgatta
    27721 caacttcaac gccacgaccg gcgaaatctt cttcctccgg cccatctcca ccttcgacta
    27781 cgccttaaat ctcatgcagg tcgtcgtcac ctacgagcac cgcgccggtg ggatgtcgtc
    27841 ggccgtctac acgggccgcg tcgtgaagaa cttcgagggc gcgggcctca aggtcggcct
    27901 ctccgtcgtc gagcagcagc agtcggagtt cggctccttc gtcgtcggcg gtatcgacgc
    27961 cgagaagaaa ctgccggccg gcgggcgcgt gcgcgccgag tgggcgatga gccgcggccg
    28021 cgtgctcttt agcggcaacc tcttctccgc cggcggcggc gaaacggatt cgcgccacga
    28081 cggcagcgcc ttccgcgccg agttggagca gccgctccct tggcgcgaga cacgcctgcg
    28141 cgcgagcttc ctgcgcgccg acgagggctt tatgaatccg ttcggcgcaa ccgtcacgcc
    28201 cggctcgcgc cgcgccgagg tctcgttaga catgcgcgtg cgtccctctt cggccttgcg
    28261 cctcggcgtc acggacgagc gcaacaagac cgccaacgtc aacaacagcc gcctgaccgg
    28321 ctccgtccag tggacggaga ccgttaaaga ccgcctgcgc gccaccgtcg cctacgacta
    28381 ccgccacttc gaggatgagt tgggcgggcg cgaggtcaac tcgaacctgc tcacggtcgg
    28441 ggccgagtgg caggcgaccg acaagttgca gctctccgtc aggcgcgagc agaatctttc
    28501 ggaggccgac cccacctacc cgaaccagac gactctggcc gccaactatc agtggagcca
    28561 gtttacgcgt ctgttcttca cccagcgcct cggctccgcg gccatcgtgc ccatcggcga
    28621 cttcagctcg acggggttcg ccgtctccgg cgcacggcgc gaaacggccg tcggcatcga
    28681 gacgcgtctc ggccgctacg ccaatctcgt cagccgctac cagttggaga acggcgcgaa
    28741 cggcactgac agcttcgccg tcatcggtct ccagaaccgc tttgcgctga ccaaagagtt
    28801 gcagctcgac ctcggctacg aacgcggctt ccacctcgcc ggccagggcc agagcttcaa
    28861 caacctccac gtcggcttcg gctggcagcc cacggccaac ttccgctcct cggcccgcta
    28921 tgaattgcgc gaccgcggcg gcgtcggcaa cgtcctgacg ctcggcgcgg ccggccgcct
    28981 cgccgacaac ctgacgacgc tcgcccgcta ccaactctcg cgcgccaact tccagggccg
    29041 cgccaactcc tccatgagcg cgcaggccgc cctcgcgtgg cgtcccctgc acaccgaccg
    29101 cgccggcctc ctcttcagct acacgcgccg gcaactcgaa caggagggcg cgaacaactc
    29161 cggctccacc ctcgaccgcg ccgacatcat ctcggccgac ggcttctacc aactgacgaa
    29221 cgacctcgaa ctctacggcc gcttcgccct gcgctacagc gacacgggga cgcccgagct
    29281 tgtgcgcgtc tcgtcgctcg cgtatctttc gcaggcgcgc gccgcctacc gcatgggccg
    29341 ctacttcgac ctcgccgccg agacccggat gttgattgcg ccctcctcgg gcacgcgccg
    29401 caactccttc ggcgcggaac tcggctactg ggtgctgccc gacctccgct tcggcggcgg
    29461 ctacaactgg acgggcgcga ccgagccgac gggcggcgcg ctcatcggcg gccgccgcgg
    29521 cttctacttc accatctcct ccaagctctc gaacctcttc gacctcttcg gcgcctcgcg
    29581 cgcgagcgaa cagcagatcg tcgagcctga cgacgcgccg gacggacagg ggaagccgaa
    29641 gaaggaagac gacgaagagg aagacgagga gggcggcaac taacccgccg aagaccttga
    29701 gacgtgcggc ccaaacactt tcgtggttcg tcggtcggcg cctgcgcgcc gcggcccccg
    29761 ccccgcgcgc cgcacgcacc tccagcgccc gccgctgggc gctctccctc ctgttcgccc
    29821 tcctcggctt ccaggccgcc cacgcttccc tgagcgtcac gcccgttacc tggaacgtca
    29881 tcggcctcga cagcaacaac gtcaactccg gcccgaacac ctacctcgtg ggcgtgctcg
    29941 tctgcaacac cggcggcgcg gccgtcacca acctcacggg cacgctcgtc tgggacagct
    30001 caaacatcta catcaacacc gtggggctga accccgtcac ctacccctcg ctcgccgccg
    30061 gcgcctgcac caacgtctac ttcaacgtcg ccgtcacccg caccactttc gcctacaaca
    30121 cggcccgccg ctaccacatc gagatcaccg ccacgggcga gccggtctac agcacgccga
    30181 cgccgcgcga aatctacgtc gagaagctcg tctcgcagaa ccgcaaccag acgctctcga
    30241 tctcggggcc gacctccctc atcgtcggac agacctacac ctacaccgtg accacgaaga
    30301 cggcgacggg cggctacgag cagttggaga cgttcctcaa cttcccgaac accatctttc
    30361 aggtgctctc cgtcgccgtc acctacacgg cccccgcggg cgcgaccaac aacaaaatct
    30421 acgccgacgc ctgcgggtgg gacaacgacc ccacccacgt cgcgcccaac cccatccaga
    30481 cctacaggag ctgcatcggc cccgtgaact accccggcgg caaggccggc gatcaggtcg
    30541 tcaccaccta cacggtcaag gtcgtcggca acgggacggc gacgatgaac tcgatgatct
    30601 acgacttctc cgggagcagc taccactaca gcaacgacta cggcccgcaa tccctgaccc
    30661 tcacctccca aacgccgccc accgtcccgc tcgtcaagtc ggtcagcccc aacggctcgc
    30721 agccgccggg cgccgacctc acctacagca tcgccttcaa caacaccggc gacctcgccg
    30781 cgcagcagtt cgtcatcacc gaccccgtcc ccgccgacac cgacttcaag atcggctccg
    30841 tcacgaccac tctcggcagc ctctcgggcg tcaccatcca gtactccacc gacggcgtga
    30901 gctggtcggc cgcgctgcca ccccccaccg gcaccgagcc tgcgggttat aatcgcaacg
    30961 tgcgccacgt ccgctgggta ttcaccggca cgctcgccgc cggaagctca ggcgccggcg
    31021 ccttcacggt gcgaatccgc tgaagccatc gccacgcgcg ccttcggcgc cgtcaacatc
    31081 gtcatccgca tcccctcctg aaccgtcgcg ccgtcgaaaa cctggactga aaatgaagcc
    31141 cggcgacccc gccgggcttt tcacttggcg ggcgtcgtcc gtgctacagt aggcgcgatg
    31201 tcaacagaag gtgatggtaa gcgtctgacc gatttaacgg tcggcgacgc gaggcgcgcg
    31261 ctcgtctacg cggcggcgac gctggcggcc gtcgtgctgt ttctggcgct cgtgtgggag
    31321 gtggccgtcg ccctgatgct cggcgtggtg gtgggcgcgt acctgctgcc gcttcaggag
    31381 ttcttcgagc gtcgtctgcg gcgcagcacg gggagcgcgc tcgtgacgat ggccgtcatc
    31441 atcatcccgc tcgtcctcct cttcggctac gggtggtacg agatgtccgg ctactcgacg
    31501 gtcgtcgtcg agcgccgcga cgagatcgcc gactccatca gccgctcgct ggcgcgctac
    31561 ctgccgctcc agtacgagga cacgcgcgcc agcctccaga cggccttcac cgaagccgtg
    31621 gtgcgctcgg ggcaggccat tggcgcggtg cgcgaaaaga ccgcgctcat catcacggcc
    31681 gccacgctct tcttcttcac catctactac gtgctcacgc agcggatgca actcgaagcc
    31741 tacatcaagc tgcgcgtgcc gggcgacttc ctgccgctct acgagaagct ggaggagagc
    31801 gtcggcgggg cgctgcgcgg cgcggtctgg gccgtcttca tcgaccagac ggttaaggcg
    31861 tccgtcatct tcgtctgcaa cctcgtcttc ggcgtgccgc tggccgtggc gctcgcgctc
    31921 accgctttca tcaccggctt ctttccgctg ctcggcgtct ggatcgtcta catccccatc
    31981 tgcatctacc tgctcgtctt ccgcggcgac gccacgtcgg ccgccatcta cttcggcatc
    32041 ggcacctcgc tcaccttctt ctcgtcgctc ttcctccggc cgatgctcgc cgccagccag
    32101 acgcgctcgt tcaacttcta ctggatgctc gtcgccttag tcagcggcgt ctacgccttc
    32161 ggcgtccccg gcgtcgtgct cggcccggcc atcctcggct tcgccaaggc cgtcatggac
    32221 acgctcgtcg gcgaggtgcg ctacgaaacc tctctgctca aggaggagaa ggtgcagaag
    32281 gccgaacagg aggcgacgga cgcggccgaa gcaaagtccg cgaccgccgc cgactgaagc
    32341 cgtaagaatt aacatggatg gacaggacag acaggatttg caggacgaac aggatgagat
    32401 gatttgaaat ctgaaatctg aaatctgaaa tctgaaatca acctttcttt atcctgtccg
    32461 tcctgttaat cctgtccatt catgtttgaa tttatgccgg atcagaatct cttcaacgtg
    32521 agcgcggagg tcgccgaggc gacggccgcg ggccgcgccg tcgtcgcgct cgaatcgacg
    32581 gtgatcgcgc acgggctgcc gcgccccttg aacgtcgaga cggcgcggcg gctcgaacgg
    32641 gtagtgcgcg agggcggcgc cgtcccggcg accgtggccg tgctcggcgg gagagtatgc
    32701 gtcggtctgg acgacgcgca gttggagcgc gtcgctttcg gcgagggggt gcggaagctc
    32761 agccgtcgcg acctcgcggc ggccgttgcc tgcgggtggg acggggcgac gaccgtcgcg
    32821 acgaccctgt gggccgcacg gcgcgcgcgt atccgcgtct tcgccacggg cggcatcggc
    32881 ggcgtccatc gcggcgcgct gcccgacgtt tcggccgacc tgccggagct tgcgcgcacg
    32941 ccgctcgtcg tcgtctgctc gggcgcgaag atcgtcctcg acttgcaggc gacgcgcgag
    33001 tggctggaga cgtggggcgt cgccgtcgtc ggctacggct gcgacgagat gcccggcttc
    33061 tacacacgcc ggagcggcct cgcggtggac gtgcgcgccg acacgccacg cgacgtggcc
    33121 cgcctcgccc gcgcccgcga cgcgcttgag ctggaaggcg cgctcgtcct cacggtgccc
    33181 gtgccggaag agtttgaggt ggactacgaa ttgttgaaga agacgctcgc ggacgcgctc
    33241 gatgaggccg agcgcgcggg cgttgcgggg cgcgaactca ccccgtttct gctctcgcgg
    33301 atgagcgaac aaagcggagg cgcgacgctg cgggccaacg tggcgctgct ggagaaaaac
    33361 gcccgcgtgg cggcggaagt cgcaggggca ttggcgtccg cgagctgtta gcgggcgtct
    33421 ggtcggtcgg ccgccggtgt gcgtcaataa acgccgaacc ctgcggccgg tttcgtcgtt
    33481 cacctacgtg aacgagttag acgaggaacg gcccggtaga ataatcacaa cgcggagcaa
    33541 aactgagtaa tgaaacgccg aatatttttc gccatccttt tgatcgcggc ggcctggttc
    33601 gccgggcgcc acttcacgca ccgccatcag tcggccgagg cggcgggccg cgacgaaacg
    33661 cacgagacct tcgagatgca gccgggagcg cgcgtcgagg tacgcggcat caacggcaac
    33721 gtgaaggtcg agacttcggc cacgacgacg gccgaggtac acgtcgtccg cacggccgac
    33781 aacggctcgg cactcgccaa ccggcgcatc tccgtggagc agaattcgac ggggctggtc
    33841 gtgcgcggcg aaagccgcgg gggcggatgg tggcgtcgca tcttcggcgg gagcggacag
    33901 gcgcgtgaag aggtgactct gcgcgtgccg cgccgcgtca accttgagac acgcggcatc
    33961 aacggtcagg tcgtggttgg cgagatcgac ggctcggtcg aagtgagtgg tgttaacggc
    34021 tcggtcgagc tgggacaggc gtccgttcgc gccgagttga acggcgtcaa cggtcaggtc
    34081 acgctggcgc tggatcgcgc cggcgagcgc ggcatcggga tgagcggcat caacggcgac
    34141 gtggagtttc gcatccgcga gagcatcaac gccgatgtcg aagtcacggg gcagaacggc
    34201 ggcgtcacgc tcaacgtgcc caacgtcacc cggcaggagc gcccgaaccg ctcgcgcatg
    34261 agcgcgcgct tcggctcggg cggtgcgcct gtgcgtctct cgggcatcaa cggcaacgtc
    34321 agatttcaaa cggccgcccc caccggctga cacgcccggc gcgcgacggc gaccgtccag
    34381 aagttttcac ccggccgcaa ctgcctgacc cgtaagttgc ctccaagtac caacggcccg
    34441 cgctgatgaa gcgcgggccg ttggttt
//
LOCUS       DASVES010000448        31756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_113259, whole genome shotgun sequence.
ACCESSION   DASVES010000448 DASVES010000000
VERSION     DASVES010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 31756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31756
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113259"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..375)
                     /locus_tag="VF240_18560"
     CDS             complement(157..375)
                     /locus_tag="VF240_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177266.1"
                     /translation="MSRFDMFGMLFFGIICLSALTLILLIMLLAKQPPSRWRNLAMAA
                     VVIAFIVGTPTLLYILLVTNYLFFDAWH"
     gene            complement(541..1257)
                     /locus_tag="VF240_18565"
     CDS             complement(541..1257)
                     /locus_tag="VF240_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177267.1"
                     /translation="MRNIIFGIAGAALGFLVGFYAANTISQQTPAPRAAARVGAAPAS
                     AGQLPPDHPEIGPAAGAVGGASSTVAPEAQAAADAAERSPRDFAAQLKAAETFYRLND
                     YRKAEQYAERAAELKPGDFKTHVLLGNSRYDRKDFTAAAEAYERALSINADDPDVRTD
                     YGNTFFLRQPPDLDRAIAEYRKSLAVNPRHEQSWLNLASASVQKKDKQTALEALARLE
                     SVNPNNPSLPTLRQSAESLP"
     gene            2099..4405
                     /locus_tag="VF240_18570"
     CDS             2099..4405
                     /locus_tag="VF240_18570"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX7177268.1"
                     /translation="MVQVTYPGVYIQEISSGFRAISGVSTSIAAFVGMAKRGPLLTPT
                     RVLGLKDYERIFSSDTSQGEMTDQVRQFFINRGEQAFIVRIADGAQRAFTVLANEAGA
                     VVLRLDSRDAGLDANQLRARVDYNTASPERTFNLTIFRETFDASGLPVVSAVETHADL
                     GMDANGARYAQTIINQSSQLVVADVNEVNVSAADINAYSASATIFATDAAVEAAVVAA
                     IGGAGVGRFRIKVGNAPWLTVGLDSAGFVFANIAARINTALAPHTTVTVTTAAIPAGG
                     GPLIIEASTTGSDIVIERASQLDIAQALGLGVAQGGIEVGSFAPARPAPSSLVSEVDG
                     GAGDLAPLLALGGVAKAALDNTGVAGLAGVRPFTVPNIVYPSAAGNLNQGTTAGLSLR
                     NIRENLEAIANALSAGSGNNWRAEVHGHRLALIPAFGDSSSGLAAAFTSTAPDLSGAN
                     QIFNGITAARAATALGGGTDGGDPQPSDYEAAYQQLDEKVDLFNMLILPKSADDTANP
                     SVRSTLWGAASAFCQKKRAFLIADVEPVLQTPDAVLLELPDVRTGVAKDHTGVWWPKV
                     TIVSGGVRKNIDPSGSIAGIMARIDGSRGVWKAPAGLEADIRGVLGVEAPMSDEQNGL
                     INPEAINAVRSFPAGVVSWGARTLDGFDNSGNTDYRYVPVRRFALFIEESLVRGLRFA
                     IFEPNDEPLWAQLRLAGGAFMNSLFRRGAFAGATAREAYFVKADAETTTAQDQALGIV
                     NVLVGFAPLRPAEFIVITIQQLAGQVQV"
     gene            4436..4939
                     /locus_tag="VF240_18575"
     CDS             4436..4939
                     /locus_tag="VF240_18575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX7177269.1"
                     /translation="MAKFTKNQRIDPYANFKFLLFFEGRPDPVAGISKISPLKRTTEV
                     VSHRDGGDLSTPRHAPGASKFEAITFERGVTHDPDFEDWANLVYSTDGDAAVSLAGYK
                     RNLLIKVNNLQGVPVKAYRVFRCWVSEYTALPELDANANAVMIESIVVQNEGFERDED
                     VPEPTET"
     gene            4973..5770
                     /locus_tag="VF240_18580"
     CDS             4973..5770
                     /locus_tag="VF240_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177270.1"
                     /translation="MDLRTHAGRGAGVPRRVYLRELSGYDELASGSAVELIDRLLVER
                     PGAAARPGDARRLTLAEADRVLAAVYRSLYGDDIECHVSCPACRSSYALSFTLTDMWT
                     AVTETTPEDEQLLDELAGPDEDGVYHLDGFRFRLPTGEDLAEVAGLGTEGMAEALRAR
                     CVLEENAERGEETLDHAMTLAGPTLDTDLDGVCPECGAEQAVPFRIDEFLFAALRRES
                     ALLTREVHELARAYRWSRREILEMPRRERRQHAGLVLAAAAPDGGWA"
     gene            5767..6660
                     /locus_tag="VF240_18585"
     CDS             5767..6660
                     /locus_tag="VF240_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177271.1"
                     /translation="MKYLLRAARRTELAGASPRARPAIPIRSPLAEADQRLNIDSFAA
                     RFDAPAVAERAAPHTPEEEPFPVPASERQAGTSAAAAESRPTPSAAPAAPRRSRGETS
                     HEPARGKDVRESQQHTAPPHTTRDARPRGRLSPGDTEDKSAPLLSARPVRRVNELPGP
                     LHAEGDGPVPERPRVNEQAKTAKGEQPPSGNMLDALSRALAWVEGDARRTAEAERAEV
                     QATPPAPAPRARFDERERGRSSEAAQRDLRPVTHLEIGKIEVEIMPPAKPAQNAAAPR
                     REPRTNGLSYPRRQPFGWRQR"
     gene            6665..8062
                     /locus_tag="VF240_18590"
     CDS             6665..8062
                     /locus_tag="VF240_18590"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX7177272.1"
                     /translation="MPLTDIARVTTAIATLITQVLARDNVADLNVTAAPPEDDTDATP
                     NVISLYLFHIAEDSFRKNLPPRRPVISDSPVQFTEMGLILNYVVTARNTSASVVGIRT
                     LNEQRLLGFVARALHDFPVITDETVVPPNAPIFQTANIAGADNRIELILRPVGIEETV
                     NFWSAEQDLTARLGLFYEARVILLSTPPITSTPGIVYSLGAYVSVAGQPFLLSVQNTI
                     GFTLPPGFSPPDPASPFQFVSSNPARVSLFPAVAVPAGVAPDNNRFRLDGSDLRGELT
                     FLALRGQAGEGANPPVERSFRIDVDDGNNPDWSFDVRDTEITVRMRQTLVDDEGTALT
                     LYPGLFSLQVITARQLAGDTTGRRLEQSSNELVFAVTPQVVSVAGMGGPASARQFRLT
                     LFGNYLRNELDIQLSVGGRVLRRDAATTVAGNFDFAPNTNQIDFAVDTSGVASPMFVN
                     LLINGAGATPAWAEF"
     gene            8062..10152
                     /locus_tag="VF240_18595"
     CDS             8062..10152
                     /locus_tag="VF240_18595"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177273.1"
                     /translation="MTAGMKEDAAAMLDAPPAPEKTGLLDAVLARMRPLAARRLLWLE
                     SLAGAGAAAEYASLPPRLRVALLDLDAPEEEARFYRRDARCRELSAQADTCADAIADS
                     PDNAFRHVIEVLGATPPEADLLQVCLAAHLDPSLAQIYGYLEGDVARRSYATEALAAR
                     LCGYGRASLWSPAGALARWQVLEADASEPGESPPLRVDPYIVQFLQGSMEIDAELLGC
                     ASYVEPRTPLPRWPVEELSRRIVETVRRGSPNRIWLVGQRLSGRRTLAACVARRLGSD
                     VVAVDTSRIGEASWSRAQVRIRRHALLHGCAVAWHGAQVLRAASAGGEMKLPLEFAVL
                     EPPGETPAEPGWREERVEMPRLLSHERARLWEQFLPASKLWTEDERRPLAERYLLQVG
                     EIAHVAAQSPESFNDVRRLAREFSRGRLDDLGHLLDCPFRREDLQLPERLGRIIDEFL
                     FEARDRIRFWENEGVRRLFPRGTGLIGLMSGPPGTGKTMAAQVIAAELELDLYRIDLA
                     STVNKYIGETAKNLRRLFARAADMNAVLLFDEADALFSKRTEVRDSHDRYANADTNYL
                     LQLIEDYPGVALLATNKRQNMDEAFVRRVRYLLFFPKPDAAQRLAIWRQVVRELAGEQ
                     CEQALAKELAHAAERVEATGAQIKNATLAAMFLARQAGRPLGSEELRRGLERELSNQG
                     GTASFESDDRGGRR"
     gene            10149..10433
                     /locus_tag="VF240_18600"
     CDS             10149..10433
                     /locus_tag="VF240_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177274.1"
                     /translation="MKVSSKGAKAAAVTRVRIERLRLRLPAGDARSAKTLAQAIASRL
                     ALRAGDLEGFAGEASLRARVKAPASSSTESVADAVVGKIAGTRGQKRGGR"
     gene            10434..11129
                     /locus_tag="VF240_18605"
     CDS             10434..11129
                     /locus_tag="VF240_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177275.1"
                     /translation="MPETGYTRSPRLVRGALVQLVEDVVAISPNVIPFQYNPETLSRK
                     LTPWNPFEVEQTGRGQIAPTAQPFDPKESISLEINFDAADQLEESDPVAGLIGVADRI
                     AAIEKLLLPTQGLIGDLIGAAAALAGAAQPPKRPTVPVCLLVWGPGRILPVRVTDYGI
                     DETTFLPSLQPLTAKISLSLEVLTPDVFRCETGPAIELAVAAYRFYRLQQSAMALDYN
                     ARNAAQSLSLLPF"
     gene            11144..11431
                     /locus_tag="VF240_18610"
     CDS             11144..11431
                     /locus_tag="VF240_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177276.1"
                     /translation="MAVFDPKSRYVTPPLEVYSVIDRRGREVKALPMVEPPRERSVGE
                     YVKKQGQRLDHLANSFLLDPHGYWRFAEVNRVIVPDALEEVERLKVPALTR"
     gene            11445..12587
                     /locus_tag="VF240_18615"
     CDS             11445..12587
                     /locus_tag="VF240_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177277.1"
                     /translation="MRDIRYQIAFDRQPDNALTDEAAEIEVQQTIEGPTTFRIKFSID
                     ICQGDFDLLNDARLNPGANDPEITVTAYLNGASEVLAHGIITGRKVNLTEGGAGSSLE
                     ITCQDRRIVMNRQPRTVAHDGFASDIVEKILSKADYKLEADVTRTKIEYQEDKVKLNQ
                     TESDLAFVDKLAGRNGFRFWLDWKAAEGLTGFELTETAHFKPSPPRPAANGPGFTPPV
                     LLAPPGAAVLKLNTGEGCSNVASFELQTNAEAPNKSASIAGLDPDSGEKVETDAKPSN
                     EPLGAQPAAPDKPREARLVTPGSDIQEGQVRTEAALNDASWSVQATVETSVQSLDAFV
                     APHQIIRLEGAGKLNSGDYFVKAVTHTIDTTAHKMRIELLRNALGG"
     gene            12587..13126
                     /locus_tag="VF240_18620"
     CDS             12587..13126
                     /locus_tag="VF240_18620"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX7177278.1"
                     /translation="MLEETDRLISDLGSHSHYYGKYRGLVVDNRDPTTKGRLRVTVQA
                     VLGDEQVWAMPCVPYAGPSLGFYMLPELGTGVWVEFEGGDVSYPVWTGCFWNAGDIDS
                     ADSDPSIKFIKTNKFTLRIDDGNGEIIIENDSGSQIKMTAQEIVIKSSAVNQEAAAGR
                     KTELTAASFRVNNGSVEVL"
     gene            13127..13504
                     /locus_tag="VF240_18625"
     CDS             13127..13504
                     /locus_tag="VF240_18625"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX7177279.1"
                     /translation="MSAPFEGFFYPVAVDASLGRFRKETDYPAYVVQLIKQVLLTAPG
                     ERVDRPDFGAGLRRHLFAPSSNETATLLQATVFQSLDRWLGNLIKVEGVDAAFENGRL
                     DIRIAYTLKARGDQQVLNLEVTL"
     gene            13504..16107
                     /locus_tag="VF240_18630"
     CDS             13504..16107
                     /locus_tag="VF240_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177280.1"
                     /translation="MPAPGDRLAKLRERSEVLTGIEFVQVVDKCDQKRLRVYFLTDPR
                     DLGTPFEDVGDATITPEPLAPSDIRIYSPRGAASDVEVVVCPGPDAMLWDDDDEAGRR
                     YLEICVAEPGDFTEYRLLIDDERVDRFFNDVQFSFKVGCDDRLDCVEPLKECVSDDLV
                     DFPVDYLARDFVSFRNALLDFAAQRYPDWQLPVEADVGVMLLEAMAALGDELSYLQDR
                     HNREAYLETATERRSLRRKARLLDHEIHDGRMARTLLELRVRPGVTSVEGGWPVWAPV
                     EGSEPIRFELGEGMRDRGKNFPVNELWNSTNFTVYLFDDGQTCLEAGATEVFVRNDAL
                     NPGDPGGVVFDEAAANLWSAGRLLLLRDRPADPSESDRLHVVRVVDVRLEHDDLFDVD
                     VARIRWEAEDATPFAIPFDELQLSGNIVPATAGESRTARFRLGPLTQSDITNNSSSAS
                     VVEIYSAVEREGPLYSEAGAGLASRRNQCEEVEEETASRPPIYLLSLPGAEESGLAFA
                     DTEGDLRRARPEVRVFEVDVTGGTDDEWKFERTLIDVDGDKDVFTLEDGTWRRIVGRW
                     LGGQEIVHRDYASGAGYTVRFGDGEFGELPPRDALFEVQYRLGSGAKGNVPAGAVAAL
                     RIPGEFSPLALLLDAAGNPYPVTSGVDAESEAEIKMLTPEAYKSETFFAVRPEDYGAQ
                     AEKLSFVQRAQGRFRWTGSWLSVVTAVDPVDSFELSPKNRAQVEALLGCRRQAGRDVI
                     VRDPRFVSVDLIIRLCVARSAFAAQVRARVVEALFGKGGARPRAGFFDPDNFTFGTPL
                     RRSALEAAIVAVEGVEAVTGIQIRAHGVTDFRDFEALAFEVGDEELIRVENSSLRPER
                     GSVSLILEGGA"
     gene            16104..18731
                     /locus_tag="VF240_18635"
     CDS             16104..18731
                     /locus_tag="VF240_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177281.1"
                     /translation="MSCCEDPKGSPLETIPAGLSSLPRQLRGFAEVRRDLLRALGREP
                     GPLYGWRPSGDDFGLMWLEMWAYVSDVLGFYDERAANETYIRTAVRRPSLRRIVELLG
                     YTPSSGVAAKARVAALADGAVAVTLPPATGFRSSAFGAESPQVFETTTAQTIHPLKNQ
                     WKVTSFKRRPVVDTAPDVTETSAPTDQKKAAPAVGTPNVRALLFETAGFGLAAGEMVL
                     LDSRQVVEENPVEPPVSLVTASEPFAGKDSVTYIRATLEPGVRIPADTDLSTLRARRP
                     TQTATPTVNQPVGPGGGKGKGTTPAVRNVSGKTHVFFDQTPGAFRKSDPIIVARNLGG
                     REAEYRFATVASAQPAAVKLTNIPEQKVSVPQQSGPDKTVTIPSPTVAATELVIEPAL
                     PLSFINHADELKFLHTFVEGGQPTNVGKTEVSAADLADPKGIPLEGIVEPPPEAAATA
                     AAQGLAQTSNVTGILEQEFLISDAARNGVAASGRITFTADGRASFQALALAQLPASSL
                     RLPLTIYGNVVETTRGESVFGEILGAGDARIANQRFKLRKKSLTYLPLATPGGNAAQV
                     STLQIRVDGVLWKQVNTFFGAGPEDAVYTVRHDDELNTYVTFGDGRRGARLPSGVQNV
                     VADYRFGAGAASPPAGSINQLAGAAKGLRAVRGPVAATPGKDPDQPEHLRTNAPRTAL
                     LFGRAVSAADFEALAREQAGVVRAKAEWLWIPSQMQAGVVVQYIGEAGSVAEALRAQA
                     DPTIPIEVTKAQAIPTTVSIGVEVDARFVKETVAAAVQKKLTEPGTGVLSREQAGIGG
                     TFWPSVLYEAVAQVEGVLAVSGVTFSTPSGGPTISNSKGTCIETGKYLDFTGEGAVTV
                     TGVEPLDSKPRPFAGGTTL"
     gene            18756..22547
                     /locus_tag="VF240_18640"
     CDS             18756..22547
                     /locus_tag="VF240_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177282.1"
                     /translation="MSDANPVRDGYESYYTEKLWQLVPEVYRHEDGLAANPGVLRRLV
                     ELIGDEVARSRRSIDRLWEDQHIETCDDWAVPYIGDLVGARLVSANDRRARRVDVAKA
                     INFRRRRGTPDLLDSLVRAMSNWDVVLVEGFRRLARTRHRLDPVPRAGGFFTGTLSGG
                     TADLRSPAGAEVSDGPFDEYFHTLDTRLLRGRDGRFGINKLNFHLYRLRAYEMTNVDP
                     VLMTDPAGPGFPRIYTIDPSGRDVQLFIDAAPPDPDAVAALPGQLSRPACGLPREWEV
                     TQAMRCRLLGHVAHVVTASHVEELRQLPNPPGAADEQALFRILEVRFESERALRRRLR
                     DLGAGIAVNPPDWYRELVTLSLLEQTGKANLYPAQVEISAVGVSGRFEPFAGVRVAAA
                     NLASRECQPDPENNLATLLISPEQGRFASVPPDDPANFEPFVERYHYGFSGDVGAGSY
                     PRDPIPAADVPSRVAVDGVVPGGGVLQGDGLLVSDNRTYSLSIVAATPIDNAVLQAGP
                     KLRPYVVLSDGGANLRPLAINTTFLIDGGWYGAGDPSNPPAAGAVADFVIEGAASAAT
                     EAFDFERVEIRFATLDPGGVRADGVRIPPLRLLVRARVRQLIIRRSITGPIHVVRDNP
                     DDPSMVEELLICESIVDARETIDRLAVSSNFGTVVIEGSTIFGDVAADVLKASDSIVD
                     GQVRVVNNQAGCFRFSASEPGDDVRLPPRYRDAFAPIPESAFNSTTFGDAQYAQLSVV
                     APPALLRGAENGSEMGVFSHLLSPIRLASVRAKVNEFGPVGLLAQYLFEGDYRAQSLV
                     EFGVLNPTPEESPPPPPPDPGDVIPEPPPEEEEPLPTTCPDEEPPTGEEPPPQIAELT
                     IVNINGAPEFWRDVDWREEVFPAPNPNGVRREGVPFDRVRVLLEYDARFGTPPDEQGW
                     TPNNEEAAALYTLDGRGGLAFRIDNRPAFFTAQAELDEELPAQMHAYASVFLDEVPEA
                     RAVPFDGFEQRVEVNDDKRPSRGMRADWLRTSERTTDGRELHYLTLDGREILGSPKPA
                     REARGWHELSMQCCFSDDDKGGCLSGLIKMKLPNDAKANAEARDAKANAEDDVAVNAA
                     RRIEGDGRALLSFDAHVDELKLRKFGESRGDLGRASLRAVFGFEGKTGQMAGRLRNFV
                     VSAPGRYVRAWMTGNTPSRAPMLRLAFVTQERVTAGVRFRVRYGRQKKNDPTLPLRVA
                     EATLKLVDTEPGRETLLEIPLPAVRAKDTVVLTLERDSSHTDDTLETSIRMMSAALVA
                     R"
     gene            22569..26279
                     /locus_tag="VF240_18645"
     CDS             22569..26279
                     /locus_tag="VF240_18645"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7177283.1"
                     /translation="MPNADISRYLNQPGKHYKGARLQQGRALLDSDFNEGAEARSDDL
                     RASLTDIIGAAASRDDGFMPALKVGDVVNSKLVRFGVTTQAYVLDYPLKPGSMYAGGA
                     LWEQSEAEPVVFQREFLQMGAGVAPRAALGTQRQLSYLRGWEQSVTAVEDGELQEPSL
                     GGADAAARVRRVRRVEVRNVQATECRAAFDEVLADLGDGDTATYDAATCELRSNARLR
                     MTFQGAPDVECGPCHPSLEGRYLGGEGHAIRIMLTAPDRFVWAFDNAAPLYRVRLVLD
                     GGGGARVEMQTPPKDAYHHPRQNMVVEFLPWEALLENGKPQGGKIAGETVRNERIAAR
                     VGLLAETDGPYDAAARTFHVRFAPGSAPQTGVGKGQTNSKAQSQPAANVKTGDGPSND
                     GVALRWDAQHPFDEQLNPTDTSPDGLVTYMYMRVWHLKSDAALTIPTTSTRPLGQTGL
                     VPVFTGRGRHGDFWTITVRPEAPDEILPREIMLPGGRPPHGPREVVTQISLVTWVSLT
                     GSVHKVVSIEDCRPTLPALTERGCCTYDVGPGGDFETIQAAVSALPVSGGRICVRPGV
                     YREEIRIAGKSNVVVTGCGGARTRVVSPETATGDALIDIDLAGDERGVTLRDLAIDAS
                     GQIGVRVAGGSRVELRRLEFAQAAGEGQGRVTGLRSAIQIIGTTDVRITRCRMELAGT
                     FSQHAAVYIDTPGGALVEGCHIETRADQAGGVSFAWGGIEVAGASRDVEIRDNVIRGG
                     RGHGVTLGGVVYRALDGVRLGVEGAGRGQSNPAPPFAVRGTFAVVEANPDGRGRREFF
                     PEPQAAIDELIIAGNHIEAVGGSGIASPALRVEHDARLTRPPLCFRRTTFAVTNAVIE
                     DNRILDNARQRTAIGGEREALGGIVLSDARRTTVRNNLVEGNGAGTGAPVCGICVAHG
                     EHVSLVSNRVLGNGTRTPNAPPALTRGQRYHGGIVVAAPLSATQTNFVDEAAPRNVYL
                     RRNVVEQPETVSVFVASRGACRITNNHFHSHVREGSLAVPTVLVFSSGKPWEAVDLPE
                     GEPNPARWRQPDGSREYLNGRAQDLPDGDGGALCFSGNQVITNGASDMGSSFSGFGAL
                     LVSFDHVSAVGNQFAARSPVRAPQRQPPHVMVIGLTADASANRVAEGVEQTNFSLAAM
                     GAMMTAGAGNILTHCPAVFGCENHNDRDYFVAEDNLSWFRPATAGCGPLTGPLFEILR
                     ILCNALFGRARGVNRNPNIVRDVQLIRALERS"
     gene            26296..30153
                     /locus_tag="VF240_18650"
     CDS             26296..30153
                     /locus_tag="VF240_18650"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEX7177284.1"
                     /translation="MTTNADITRLLIQPRKHYVGARMQQGRVVLDSDFNEEAALGDEE
                     RRRALLDIVGPTGSPDDGFAAELEIGDQVTIQPVSFGGAPAVNTLNYRLRPGVMYLAG
                     LRFEHEERTINNVAGGDPIVFQRDFLQMRDADAPQTAEVAHAQLTYLHAWQQWVSATE
                     DEEIRERALGRLDTSVRVRQMARVEVREAANTVDCAGAWAEVRQQIEAEAGGAFDASG
                     HELLSPARLRVTFVPGEALDTCAPCAPDDVGRYLGADNQAIRIMLAAADRYVWGFDNA
                     SPLYRVELGETEDGLVPVRMITEPRDEARWPLVNTVVEFLPWSALLDNGEKVAAETGV
                     FLRVAEGFDPDVTSFRIDAADLPQLDDLVRDWDAAHPDLSLLPNDADPDGRYLYMRVW
                     HRLDAETDDVLLDTAAAPGNHTLLRRQGLLPEFTGGGRVGDYWVVSVRPNTPQQITPW
                     NLTQGGGAPPHGPRHLYTPLTLINFRPPGAGELENTEVVESVHDCRRIFRPLVDRMGC
                     CTHTVGDGVDSQGDYLSIQTAIDGLPPEGGKVCLLPGTYTEAVRIERGDVIVEGCGRQ
                     TRWRTPAGDQNPALIHVLGPRVTLRELALETFGQIGVLAGPEDLNSDVFANDLVLERL
                     DIVADERAGVGGQARCAIDVRRGRRVLVRECEVTMEGSVSDEAGVFVRGEDIRVEGCR
                     IETARGDDGLPTCWGGLQVGGATRRVQILRNRIREGLGHGITLGSLEWVAEETAPTGG
                     RGEVDDEATRVRRVASFGAGTGLQNTFDPCAPQQDSVQPVEVDQVRFDPETAGDLEDI
                     HIADNRIEAMQGNGISVLTTLRLADDGEQDLITVDRLTIERNRISGCVVQSRTLASNS
                     PVQKTKKLSPGEEEVFGQFLVSNIPPAGIALVDGEHIVIRSNEIRDNGTTDPNPVAGV
                     CIIFGNDILIEENRIQNNGLRRPASIPSISSMRAGIAVSLAGVASNTETQNQSDTLGS
                     SLRIIGNIVEHPNGPALAARATGPVVIEDNYLLSLGNNATAQQASTAHAVAVVNAGMP
                     WESIDLPAGEPSPNRWVFPPGTPEYLARKPGDEGGDGGVGAPGEGGGIIGQGGPVLFS
                     NNHVTLRWVEASNAAAGLASGFSVGICSLDDVNMHGNQFALNVEDPGLKKKDGTTFNR
                     QPRISAHVVAVGATANASHNRVAEGVNDALISLQVLGGFLVNASYNITTHMSFAATCH
                     GIRGEEGGPANGKDEYRVYRGNLVWLRPALDESREDMVSLETVMRVSQQLFVTLCDTV
                     LGLGPDDDSTRFIAILGIFAARG"
     gene            30200..30421
                     /locus_tag="VF240_18655"
     CDS             30200..30421
                     /locus_tag="VF240_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177285.1"
                     /translation="MSEDAPRVNRAAVVAGVARDLDAHLRSLRRKLERAALRGEPVER
                     LQARIRREEEQLRRLRAYEQGQLDTRRPE"
     gene            30418..31035
                     /locus_tag="VF240_18660"
     CDS             30418..31035
                     /locus_tag="VF240_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177286.1"
                     /translation="MNRGAAIPNKRIITSTEKRQDTPAARPPTAHALRRSAARVATTA
                     TAASAAEPGGETSGADTGLSARVATAADLPWLQTWAAQLGLPAPRSRRVRSFVLLKDG
                     QRVGYMAARDEMADAGRARESMLWIVSAFLIPTLRGQGMILKFGEILSRVHYRKGKVG
                     CRVAADNTPMMRLMARGGWKKLHSTRRFTDFMLELDAPFRASRKG"
     gene            31048..>31756
                     /locus_tag="VF240_18665"
     CDS             31048..>31756
                     /locus_tag="VF240_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177287.1"
                     /translation="MPRAAAPKAKRAAKHGQSVGGDSKTAQRKRTRRPAGAASPAVGV
                     LQPKLKVSAPDDQFEREADHVSDRVMSMPAPAGAAGATVGRDQKEEEGKSVQRKEAEE
                     EKQAQTFSLQREEKEEETTSVQRQAAEEEPTEEVAQTFSLQRREDEEEQTSSVQREEK
                     EEEEKSSSLQRKEEEEQSSSLQRRAAGEAKEQMQQRAKRPRRPEITPQFETDLRLLRR
                     AGGQPLPGHLRAFLEPRF"
BASE COUNT         5316 a        10625 c        10947 g         4868 t
ORIGIN      
        1 tcatggattc ttaaacagaa gcggcccaac gccccacatc actcgccgcg cacacaacga
       61 atcaggcacg gcgtcttgcg gacgaaggcc gcgctactcg cggtcgggag catgccgtag
      121 gtaggcggct ggcgcgatta gcaagagtta ccacccttag tgccacgcat cgaagaacag
      181 ataattagtc acgagcagta tatacagcag ggtgggcgtg cctactatga aggcgatgac
      241 cacagcggcc atcgccaagt tacgccatct gctcggtggc tgcttcgcca ggagcatgat
      301 gagaaggatt agcgtcagag ccgataggca gatgatgccg aagaagagca tgccgaacat
      361 gtcgaacctg ctcatgactc acctcgtctc gtgcctatga agatgctcta ccgtaagaag
      421 cctgacgccc gaccccaccg gcccgctgct gcctttgctg gaatatgacg agtttcgtat
      481 cgcgtaaagt taaaagtgag ccaactattt tgatgatctg aaaagagtgg acaggaaact
      541 tcagggcaga gactcggcgc tctggcgcag ggtgggcagg gacgggttgt tggggttgac
      601 ggattcgagg cgcgcaagag cttcgagcgc ggtctgcttg tccttcttct ggacggaggc
      661 cgaggcgagg ttcagccagc tctgctcgtg gcgtgggttg acggcgagcg acttgcggta
      721 ctcggcgatg gcgcggtcga ggtcgggcgg ctggcggagg aagaaggtgt tgccgtagtc
      781 ggtacgcacg tccgggtcgt cggcgttgat ggagagggcg cgctcgtagg cttcggcggc
      841 ggccgtgaag tctttgcggt cgtagcggga gttgccgagg agcacgtgcg tcttgaagtc
      901 gccgggcttg agttcggcag cgcgctcggc gtactgctca gccttccggt agtcgttcag
      961 ccgataaaac gtctcggcgg ccttgagttg cgcggcgaag tcgcgggggc tgcgctcagc
     1021 cgcgtcggcg gcggcttgcg cctcgggcgc gaccgttgaa gacgcaccgc cgacggcgcc
     1081 ggccgcgggg ccgatctcgg ggtggtcggg cggcagttgc ccggcggacg cgggcgccgc
     1141 gccgactctt gcggcggcgc gcggcgccgg cgtctgctgc gagatggtgt tggcggcgta
     1201 gaagccgacg agaaagccga gggcggcgcc ggcgatgccg aagataatgt ttctcatcga
     1261 aaacgtttac tcgtgtccgc ggcgatggtg atcaggcccg cgcggccgtc gctcgcgccg
     1321 cgccgccggc gttcgcgccg cgatgataac cggcgcgccg gcctcacggc aacgaccggg
     1381 cgcgggaacg cgtgaggtaa cacggatgag acggattgca cacggatgtc cattgacttt
     1441 attgcccgca tcgtcaggtg atggccttat agtggtggcc ttatatctga cgccgatagc
     1501 ggagttcgtc gccgagtaac atttacggcg aaactcttga ctatatttaa aaacggtgta
     1561 ggctatcacg catctcaagg gtcgctcgaa aagttatctg ctaccgatag acacaagagt
     1621 atctggctgt cgttgctcct tcaatcaacg gtcactcact aaggagtaag tcgtgaggac
     1681 tcttcatcac tcgacacgct tccggccgga gaacggcgcg gccgtttccc cggagccggg
     1741 gtgtgtcgga gtgcagcccg tttggtttca cgccccaatc gtagatgccg ccccatcctc
     1801 aagaccggct cagcgataac ctcgaacctg atccgcggaa ttaatcattg acatcggtgg
     1861 cgcaagcggc acgcggggcg tagctcgcat tttttaccgc tacggcccaa gccgccccgt
     1921 catcaacgct cttcacaggg gaaaggctct tcattctttt cggctcgggg aggcacacac
     1981 gtacacgcgc gttgacatca cgtaatagtc aaaacgggcg ggcgcgggcg tcgggggttt
     2041 acacgccgtt cgcccgtccg gcgtccgaga aacaatttcc cgcaggcagg aggaagcgat
     2101 ggttcaggtc acatacccag gcgtctacat ccaggaaatc tcgtccggat tccgggcgat
     2161 ctccggcgtg tccacctcga ttgcagcttt cgtggggatg gcgaaacgcg ggccgctgct
     2221 gacgcccacg cgcgtcctcg gtctcaagga ctacgagcgc atcttctcgt ccgacacctc
     2281 gcagggcgag atgaccgatc aggttcgcca gttcttcatc aacaggggcg agcaggcatt
     2341 catcgtccgc atcgcggacg gggcgcaaag ggcgttcacc gtgctggcga acgaagccgg
     2401 cgccgtcgtg ttgagacttg attcgcgcga cgccgggctg gacgccaacc aacttcgcgc
     2461 ccgcgttgac tacaacacgg caagccccga acggacgttc aacctgacga tcttccgcga
     2521 gaccttcgac gccagcgggc tgcccgtcgt ctctgccgtg gagacgcacg ccgacctcgg
     2581 gatggacgcc aacggggcgc gttacgcgca gacgatcatc aaccagtcgt cgcagttggt
     2641 cgtcgccgat gtcaacgaag tcaacgtcag cgccgccgac atcaacgcgt actccgcttc
     2701 ggcgaccatc ttcgccactg acgcagccgt agaggcggcc gtggtggcgg ccatcggcgg
     2761 cgccggcgtc gggcgctttc gcatcaaggt ggggaacgcg ccgtggctca cggtgggttt
     2821 ggatagcgcc ggcttcgtct tcgcgaacat cgctgcccgc atcaacacgg cgctggcccc
     2881 acacacgacc gtgacggtga cgaccgccgc gatacccgcg ggcggcggcc ccttgatcat
     2941 cgaggcgagc acgaccggca gcgacatcgt catcgaacgc gcgtcgcagc tcgacatcgc
     3001 tcaggcgctt gggctgggcg tggcgcaggg cggaatcgag gtcgggagct tcgcgccggc
     3061 gcggcccgcg ccttcgtcgc tggtctcgga ggtggacggt ggcgcgggcg accttgcgcc
     3121 gttgctcgcg ttgggcggtg tggcgaaggc cgctttggat aacaccggcg tcgcgggttt
     3181 agccggggtg cggccattca ccgtcccgaa catcgtctac ccttcggcgg ccgggaacct
     3241 gaatcagggg acgaccgcgg gactgtcgct gcgcaacatc cgtgagaacc tggaggccat
     3301 cgccaacgca ctttcggcgg ggagcggcaa caactggcgc gcggaagtac acggacaccg
     3361 tctggcgctc atcccggcct tcggcgattc gagcagcggg ttggccgccg cctttacgtc
     3421 caccgccccc gatctgagcg gggcgaacca aattttcaac ggcatcacgg ccgcgcgcgc
     3481 cgcgacggcc ctcggcggcg gcaccgatgg cggcgacccg cagccgtcgg attacgaggc
     3541 cgcctaccag cagctcgacg agaaggtcga tctcttcaac atgctgatcc tgcccaagag
     3601 cgcagacgac acggccaacc ccagtgtccg ctcgacgctg tggggcgcgg ccagcgcctt
     3661 ctgccagaag aagcgcgcct tcctcatcgc cgacgtggag ccagtcttgc aaacgcccga
     3721 cgccgtgctg ctggagttgc cggacgtgcg gacgggcgtg gcgaaggatc acacgggcgt
     3781 ctggtggccg aaggtcacca tcgtctcggg cggggtgcgg aagaacatcg acccgagcgg
     3841 ctccatcgcg ggcatcatgg cccgcatcga cgggagtcgc ggcgtgtgga aagcgcccgc
     3901 cgggctggag gccgacatcc gcggcgtgct cggcgtcgag gcgccgatgt cggacgagca
     3961 gaacggtctc atcaaccccg aggcgatcaa cgccgtgcgc tcgttcccgg ccggcgtcgt
     4021 gagctggggc gcgcgcaccc tcgacgggtt cgacaactcg ggcaacactg actaccgcta
     4081 cgtgcccgtc cgccgcttcg cgctgttcat cgaggagagt ctggtgcgcg gcctgcggtt
     4141 cgccatcttc gagccgaacg acgaaccgct gtgggcgcag ctccgcctcg ccggcggcgc
     4201 gtttatgaac agcctcttcc gccggggcgc cttcgccggc gccaccgccc gcgaggctta
     4261 cttcgtgaag gcggacgccg agacgacgac cgcgcaggat caggcgctcg gcatcgtcaa
     4321 cgtgctcgtc ggcttcgcgc cgctgcggcc cgccgagttc atcgtcatca cgattcagca
     4381 actcgcgggg caggtgcaag tctagacccg tcgaaacatt cgggaggcaa tcggcatggc
     4441 taagttcacc aagaaccaac gcatcgaccc atacgcgaac ttcaagtttc tgctcttctt
     4501 cgaggggcgg cccgaccccg tggccggcat cagcaaaatc agcccgctca agcgcaccac
     4561 cgaggtggtc agccaccgcg acggcggcga cctgagcacg ccgcgtcacg cgcccggcgc
     4621 ttccaagttc gaggccatca ccttcgagcg gggcgtgacg cacgaccctg acttcgagga
     4681 ctgggccaac ctcgtctaca gcaccgacgg cgacgcggcc gtctcgctcg cgggctacaa
     4741 acgcaacctc ctgatcaagg tcaacaacct tcagggcgtc cccgtcaagg cgtaccgcgt
     4801 cttccgctgc tgggtctccg agtacacggc cctgccggaa ctcgacgcca acgccaacgc
     4861 cgtgatgatc gagtccatcg tcgtccagaa cgagggcttc gagcgtgacg aggacgtgcc
     4921 ggagccgacc gagacctgaa gggccactgg ccgagcctga aacatgatcg gggtggattt
     4981 acgcacacac gccggacggg gcgcgggagt gccgcgcagg gtctacctgc gcgagctttc
     5041 cggctacgac gagttggctt caggctcggc cgtcgagttg atcgaccgcc tgctcgtcga
     5101 gcggcccggc gcggccgccc gaccgggaga cgcccgccgg ctcacgctgg ccgaggccga
     5161 ccgcgtgctg gcggccgtct atcgcagcct ctacggggac gacatcgagt gccacgtctc
     5221 ctgtcccgcc tgtcgctcct cctacgccct gagcttcacc ctcacagaca tgtggacggc
     5281 cgtgacggaa accaccccgg aggatgaaca actcctcgac gaactcgcgg ggccggacga
     5341 ggatggcgtc taccatctgg acggtttcag gtttcgtctc ccgacgggcg aagatttggc
     5401 cgaggtggcg ggcctcggga cggaaggcat ggccgaggct ttgcgcgcgc ggtgcgtcct
     5461 tgaggagaac gctgaacgcg gcgaggagac gctcgaccac gccatgacgc tggcagggcc
     5521 gacgctcgac accgacctcg acggcgtctg tcccgagtgc ggcgcggagc aggccgtccc
     5581 gtttcgtatc gacgaatttc tcttcgccgc cctgcgacgc gagagcgcgc tcctgacgcg
     5641 cgaggtacat gagctggcgc gcgcctaccg ctggtcgcgg cgggagattc tggagatgcc
     5701 ccgccgcgag cgcaggcagc acgcggggct ggtgctggcc gcggccgcgc cggacggggg
     5761 gtgggcatga agtatctgtt gagggccgcc cggcggacgg agttggcagg cgcgtcgccg
     5821 cgcgcgcggc ccgcgatacc cattcgctcg ccgctcgccg aggccgacca gcggctcaac
     5881 atcgactcct tcgcggcacg gttcgacgcg cctgccgtcg ccgagagggc cgcgccgcac
     5941 acccctgaag aggaaccgtt ccccgttccg gcttccgaac gacaggccgg gacgagcgcc
     6001 gccgccgccg agagccggcc gacgccttcc gctgctcccg ccgctccgcg ccgctcgcgt
     6061 ggcgagacgt cgcacgagcc ggcgcgtggg aaagacgttc gcgaaagtca gcagcacacg
     6121 gccccgccgc acacgacgcg cgacgcgcga ccgcgcggga ggttgtcgcc cggcgacacg
     6181 gaagataagt ccgcgccgtt gctgtcggcc cgccccgtcc gtcgtgtgaa cgagttgccc
     6241 ggcccgttgc atgcggaggg cgacggcccc gttcccgaac ggcctcgggt gaacgagcag
     6301 gcgaagacgg cgaaaggtga acagccgccg tcgggaaaca tgctcgatgc gctgagtcgg
     6361 gcgctggctt gggtcgaggg cgacgcccgg cgcacggccg aggctgagcg ggccgaggtg
     6421 caagcgacgc cgcccgcgcc cgcgccgcgc gcccggttcg acgagcgtga gcgggggcgt
     6481 tcgagcgagg cggcgcagcg cgacctgcgg cccgtcacgc atctggaaat cgggaagatc
     6541 gaggtggaga tcatgccgcc ggcgaagccc gcgcagaacg ccgccgcgcc ccggcgcgag
     6601 ccgcgcacca acggcctgag ctatccccgg cggcaacctt tcggatggag gcaaagataa
     6661 acgcgtgcct ctgaccgaca tcgcgagggt gacgacggcg attgcgaccc tgattactca
     6721 ggttctcgcg cgtgacaacg tcgccgattt aaacgtcacg gccgcgccgc ccgaggacga
     6781 caccgacgcg acgccgaacg tcatcagcct ttacctgttc cacatcgccg aggactcgtt
     6841 ccgcaagaac ctgccgccgc ggcgtccggt catctctgac tcgcccgtgc agttcacgga
     6901 gatgggattg attctcaatt acgtggtcac ggcgcgcaac acgagcgctt ccgtcgtcgg
     6961 catccgcaca ctcaacgaac agcgcctgct ggggttcgtc gcgcgggcgc tccacgactt
     7021 ccccgtcatc acggacgaga cggtcgtgcc gccaaatgcg cccatcttcc agacggcgaa
     7081 catagcgggg gccgataatc gaatcgagtt gattctccgg cccgtcggaa tcgaggagac
     7141 ggtgaatttc tggagcgccg agcaagacct gacggcgcga ctcgggttgt tctacgaggc
     7201 gcgagtcatc ctgctcagca cgccgccgat cacttcgacc cccggcatcg tctactcgct
     7261 cggcgcatac gtgtcggtgg cgggccagcc gttcttgctg tcggtgcaga acaccatcgg
     7321 gttcacgctg ccgcccggtt tctcgccccc cgacccggcg tcgccgttcc agttcgtctc
     7381 gtcgaacccg gcgcgcgtgt cgctgttccc ggcggtggcc gtccccgcgg gcgtcgcgcc
     7441 cgacaacaac cgcttccgcc tcgacggctc cgacctccgc ggcgagctga cgtttctggc
     7501 gctgcgcggg caggccggcg agggcgcgaa cccgcccgtc gaacgcagct tccgcatcga
     7561 cgttgacgac ggcaacaacc ccgactggag tttcgacgtg cgcgacacgg agatcaccgt
     7621 gaggatgcgg cagacgcttg tggacgacga gggcacggcg ctcacgctct atccggggct
     7681 tttttccttg caggtgatta cggcgcgtca actcgccgga gacactacag gccggcggct
     7741 cgaacagtcg agcaacgaac tggtctttgc cgtcactccg caggtggttt cggtcgccgg
     7801 gatgggcggc cccgccagcg cgcggcagtt ccgtctgacc ctcttcggca actacctgcg
     7861 gaacgaactc gacatccagc tctcagtggg cgggcgcgtg ctgcggcgcg acgcggccac
     7921 gaccgtcgcg ggcaatttcg acttcgcgcc gaacaccaac cagatcgact tcgcggtgga
     7981 cacgtccggg gtggcgagtc cgatgtttgt caacctcctg attaacgggg ccggcgcgac
     8041 gccggcgtgg gcggagttct gatgacagcg ggcatgaaag aggacgcggc ggcgatgctc
     8101 gacgcgccgc cggcgccgga gaagacgggg ctgctcgacg ccgtgctggc gcggatgcgc
     8161 ccgctcgcgg cgcgccggct cctgtggctc gaaagcctcg cgggcgcggg cgccgcggcc
     8221 gaatacgcga gcctgccccc acgcctgcgg gtcgctttgc tcgaccttga cgcgccggag
     8281 gaagaggccc gtttctatcg ccgggacgcg cgctgccgcg agctgagcgc acaggccgac
     8341 acttgcgccg acgccatcgc tgactcgccg gacaacgcct tccgtcacgt catcgaagtc
     8401 ctcggcgcga ccccgcccga ggccgacctg ctgcaagttt gtctggcggc gcacctcgac
     8461 ccgagcctcg cgcaaatcta cggttacctc gaaggcgacg tggcccggcg cagttacgcc
     8521 acggaagctc tggccgcgcg gctgtgcggc tacggccggg cgtcgctgtg gagtccggcg
     8581 ggcgcgctgg cgcgctggca ggtgttggag gccgacgcgt ccgagccggg cgagtcgccg
     8641 ccgctgcgcg tcgatccata catcgtgcag ttcctgcaag gcagcatgga aatcgacgcc
     8701 gagttgctcg gctgcgcctc ctacgtcgag ccgcgcacgc ctctgccgcg ttggcccgtc
     8761 gaagagttga gtcgccgcat cgtcgagacc gtgcggcgag gatcgccgaa ccgcatctgg
     8821 ctcgtggggc aacgcctcag cgggcggcgg acgctggcgg cgtgcgtggc gcgaaggctg
     8881 ggcagcgacg tggtcgcggt cgatacgtcg cgcatcggcg aggcgagctg gtcgcgcgcg
     8941 caggtgcgta tccgccggca cgcgctgttg cacgggtgcg ccgtagcgtg gcacggcgct
     9001 caggtgttgc gggccgcctc ggcgggcggc gagatgaaac tgccgctgga gttcgcggtg
     9061 ctggagccgc cgggcgagac gcccgcggag ccgggctggc gcgaggagcg ggtcgagatg
     9121 ccgcgtttgc tctcgcacga acgcgcccgc ctgtgggagc aattccttcc ggcgtcgaag
     9181 ctctggacgg aggacgagcg ccgaccgctc gccgagcgtt acctgttgca ggtcggcgaa
     9241 atcgcgcacg tcgcggcgca gtcgcccgag agtttcaacg acgtgcggcg tctggcccgc
     9301 gagttctcgc gcggccgact cgacgacctc ggccatctcc tcgattgccc cttccgccgc
     9361 gaagacctcc aactgccgga acgtttgggg cgcatcatcg atgagtttct cttcgaggcg
     9421 cgcgaccgca tccgcttctg ggagaacgag ggcgtgcgcc gcctgttccc gcgcggcacc
     9481 ggactcatcg gattgatgtc ggggccgccc ggaacaggga agacgatggc cgcgcaggtc
     9541 atcgccgccg aactcgaact cgacctctac cgcatcgacc tcgcctcgac cgtcaacaag
     9601 tacatcggcg agacggccaa gaatctccgc cgcctgtttg cgcgcgccgc cgacatgaac
     9661 gccgtgctcc tcttcgacga ggccgacgcg ctcttcagca agcgcacgga ggtgcgcgac
     9721 tcgcacgacc gctacgcgaa cgccgacacg aactacctgc tccaactgat cgaggactac
     9781 cccggcgtcg ctctgctggc gacgaacaag cgtcagaaca tggacgaggc gttcgtccgc
     9841 cgcgtccgct acctgctctt cttcccgaag cccgacgcgg cgcagcgcct cgccatctgg
     9901 cggcaggtgg tgcgtgaact cgccggcgag cagtgtgagc aggcgctcgc taaggaactc
     9961 gcccacgccg ccgagagggt cgaggccacc ggcgcgcaga tcaagaacgc cacgctggcg
    10021 gcgatgtttc tggcgcgaca ggcgggccgt ccgctgggga gcgaggaact ccgtcgcggg
    10081 ctggagcgcg agttgtcgaa tcagggcggc acggcttcgt tcgagtcgga tgatcgcgga
    10141 ggcagaagat gaaagtttcg tcgaagggcg ctaaggcggc ggcggtgacg cgtgtgcgga
    10201 tcgagcggct gcggctgcgg ctgccggccg gcgacgcgcg ttcggcgaag acgctcgcgc
    10261 aggcaatcgc gtcgcgcctc gctctgcgcg cgggcgacct cgaaggcttc gccggggagg
    10321 cgtcgttgcg tgcgcgcgtc aaggccccgg cgtcgtcgtc caccgagtct gtggcggatg
    10381 cggtcgtcgg gaagatcgcg gggacgcggg gccagaagcg ggggggacga tagatgccgg
    10441 aaaccggata cacgagaagt ccgcggctgg tgcggggcgc gctggtgcaa ctggtggagg
    10501 acgtggtcgc catctcgccg aacgtgatcc cgttccagta caacccggaa acgctctcgc
    10561 ggaagctgac gccgtggaac ccgttcgagg tcgagcagac cgggcgcggg cagatcgccc
    10621 cgaccgcgca gcccttcgac ccgaaggagt cgatctcgct ggaaatcaat ttcgacgccg
    10681 ccgaccagtt ggaggagagc gaccccgtcg cgggtctcat cggcgtggcc gaccgcatcg
    10741 cggccatcga gaaactgctg ctgccgacgc aggggctgat cggagattta atcggcgcgg
    10801 cggcggcgct ggcgggcgcg gcgcaaccgc cgaagcggcc cacggttccg gtgtgcctgc
    10861 tggtgtgggg gccggggcgg attctgcccg tgcgcgtcac cgactacggc atcgacgaga
    10921 ccaccttcct gccgtcgctg caaccgctca cagccaagat cagtttgtct ctggaggtgc
    10981 tgacgccgga cgtgtttcgt tgcgagacgg ggccggcgat tgagttggcg gtggcggcct
    11041 accgcttcta tcgcctgcaa cagagcgcga tggcgctcga ctacaacgcg cgcaacgcgg
    11101 cgcagtcgct tagcctgctg ccgttctgag gaggagttag acgatggccg tcttcgatcc
    11161 taaaagtcgc tacgtcacgc cgccgctcga agtctactcg gtcatcgacc gccgcgggcg
    11221 cgaggtcaag gcgctgccca tggtcgagcc gccgcgcgag cggtcggtgg gcgagtacgt
    11281 gaagaagcag gggcagcggc tcgaccatct ggcgaactcc ttcctcctcg acccgcacgg
    11341 ctactggcgc ttcgccgaag tcaaccgcgt catcgtgccg gacgcgctcg aagaggtcga
    11401 acgcctgaag gttccggcgt tgacgcgcta agggggaagg gcaagtgcga gacatccggt
    11461 atcagatcgc gttcgaccgc cagccggaca acgcgctcac cgatgaggcg gcggagatcg
    11521 aggtgcagca gaccatcgaa ggcccgacca cgtttcgcat caagttctcc atcgacatct
    11581 gtcagggcga cttcgacctg ctcaacgacg cgcggctcaa ccccggagcc aacgaccccg
    11641 agatcaccgt cacggcctac ctcaacggcg cgagcgaagt gctcgcgcac ggcatcatca
    11701 cggggcgcaa ggtgaacctg accgaaggcg gcgccggctc gtcgctggag attacgtgtc
    11761 aggacaggcg gatcgtgatg aaccgccagc cgcggaccgt ggcgcacgac gggttcgcga
    11821 gcgacatcgt ggagaagatt ctgagtaagg cggattacaa actggaggcg gatgtcaccc
    11881 ggacgaaaat cgagtatcag gaagacaagg tgaagcttaa tcagaccgaa tcagacctcg
    11941 cgttcgtgga caagctggcc gggcgcaacg gcttccgctt ctggctcgac tggaaggcgg
    12001 cggaagggct gacgggtttc gagctgacgg agacggcgca cttcaagccg tcgcctccgc
    12061 gcccggccgc caacgggccg gggttcacgc cgcccgtcct gctcgccccg ccgggggcgg
    12121 ccgtgctcaa gctcaacacg ggcgagggct gctcgaacgt cgccagcttc gagttgcaga
    12181 cgaacgcgga ggctccgaac aagtccgcct ccatcgcggg cctcgacccc gacagcggcg
    12241 agaaggtcga aaccgatgcc aagccgagca acgagccgct gggcgcgcag ccggcggccc
    12301 ccgataagcc ccgcgaggcg cggctcgtga cgccgggcag cgacattcag gaggggcagg
    12361 tgcggaccga ggccgccctt aacgacgcgt cgtggtcggt gcaggcgacg gtcgagacga
    12421 gcgtccagtc gctcgacgcc ttcgtcgcgc cgcaccagat catccgtctg gagggcgcgg
    12481 gcaagctcaa cagcggcgac tatttcgtga aggccgtgac gcacaccatc gacacgacgg
    12541 cccacaagat gcggatcgag ctgctccgca acgcgctcgg aggttgatgt tggaagagac
    12601 ggacagactc atcagcgatc tgggttcgca ctcccactac tacgggaagt accgcgggtt
    12661 ggtggtcgat aaccgcgacc cgacgacgaa gggccggctg cgcgtgaccg tgcaggccgt
    12721 gctcggcgac gagcaggtgt gggcgatgcc gtgcgtgcct tacgcggggc cgagcctcgg
    12781 cttctacatg ttgcccgaac tcggcacggg cgtgtgggtc gagttcgagg ggggcgatgt
    12841 ttcctacccc gtctggacgg gctgcttctg gaacgcgggc gacatcgaca gcgccgactc
    12901 cgacccttcg atcaagttca tcaagacgaa caagttcacg ctccgcatcg acgacggcaa
    12961 cggcgagatc atcatcgaga acgacagcgg ctcgcagata aagatgacgg cgcaggagat
    13021 cgtcatcaag agttccgccg tcaatcagga ggccgccgcc ggtcgcaaga ccgagttgac
    13081 ggccgcctcc ttcagagtca acaacggctc ggtggaggtg ctgtagatga gcgcgccctt
    13141 tgaaggtttt ttctaccccg tcgcggttga cgcctccctc gggcggttcc gcaaggagac
    13201 cgactacccg gcctacgtcg tgcagctcat caagcaggtg ctcctgaccg cgccgggcga
    13261 gcgtgtggac aggccggact tcggggcggg cttgcggcgg cacctcttcg cgccgagcag
    13321 caatgagacg gcgacgctgt tgcaggcgac cgtcttccag agcctcgacc gctggctcgg
    13381 caatctcatc aaggtcgagg gcgttgacgc cgcgttcgaa aacgggcggc tcgacatccg
    13441 catcgcctac acgctcaagg cgcgaggcga tcagcaagtg ctcaatctgg aggtcacgct
    13501 ctgatgcccg ctcccggcga cagactcgcg aagctgcgcg agcgctccga agtcctcacg
    13561 ggcatcgagt tcgtgcaggt ggtggacaag tgcgaccaga agcgtctgcg cgtctacttc
    13621 ctgaccgacc cgcgcgacct cggcactccg ttcgaggacg tgggcgacgc gacgatcacg
    13681 cccgagccgc tcgcgccttc cgacatccgc atctacagcc cgcgcggcgc ggcctcggac
    13741 gtggaggtgg tcgtctgccc cggccccgac gcgatgctgt gggacgacga cgacgaggcg
    13801 ggccggcgct acctggaaat ctgcgtcgcc gagccgggcg acttcaccga gtaccggctc
    13861 ctcatcgacg acgagcgcgt tgaccgcttc ttcaacgacg ttcaattctc cttcaaggtc
    13921 ggctgcgacg accggctcga ttgcgtcgag ccgttgaaag aatgcgtgag cgacgacctc
    13981 gtggatttcc ccgtcgatta tctggcgcgc gacttcgtca gcttccgcaa cgcgctgctc
    14041 gacttcgcgg cgcagcgtta cccggactgg caactgccgg tcgaggccga tgtcggcgtg
    14101 atgctgctgg aggcgatggc ggcgctcggc gacgagctgt cgtacctaca ggatcggcac
    14161 aatcgcgaag cctatctgga gacggcgacc gagcggcgct cgctgcgccg gaaggcccga
    14221 ctgctcgacc acgagattca cgacggccgc atggcgcgca cgcttcttga gttgagggtg
    14281 cgacccggtg tgacctcggt ggagggcggc tggccggtct gggcgccggt cgaaggttcg
    14341 gagccgatac ggttcgagtt gggtgagggg atgcgcgacc gcggcaagaa cttccccgta
    14401 aacgaactct ggaattcgac gaacttcacg gtctatcttt tcgacgacgg ccagacgtgt
    14461 ctggaagccg gggccacgga ggtgttcgtc cgcaacgacg cgctgaaccc gggcgatccg
    14521 ggcggcgtcg tcttcgacga ggcggcggcg aacctgtgga gcgccggccg tctgttgctg
    14581 ttgcgtgacc gcccggccga cccgtccgag tctgaccgcc tgcacgtcgt gcgcgtcgtc
    14641 gatgtgcggc tcgaacacga cgacctgttt gacgtggacg tggcgcgcat ccgttgggag
    14701 gcagaggacg cgactccctt cgccattcct ttcgacgagc tacagctctc cggcaacatc
    14761 gtcccggcga cggcgggcga gtcgcgcacg gcgcgctttc gcctcgggcc gctcacgcag
    14821 agcgacatca cgaacaattc gagtagcgca tcggtggtgg agatttattc ggccgtggag
    14881 cgcgaaggcc cgctctactc ggaggccggc gcgggcctcg cgtcgaggcg gaatcagtgt
    14941 gaggaagtgg aggaagagac cgcgtcgcgc ccgcccatct acctgctcag cctgcccggc
    15001 gccgaggaga gcgggctggc gttcgccgac acggagggcg acctgcgccg ggccaggccg
    15061 gaggtgcgtg tcttcgaggt cgatgtcacc gggggaaccg acgacgaatg gaagttcgag
    15121 cgcacgctga tcgacgtgga cggcgacaaa gacgtcttca cccttgagga cgggacgtgg
    15181 cggcgcatcg tcgggcgttg gctcggcgga caggagatcg tccaccgcga ttacgccagc
    15241 ggcgcgggct acacggtgcg tttcggcgat ggtgaattcg gagaactccc cccgcgggac
    15301 gccttgttcg aggtgcagta ccgtttaggc tcgggcgcga agggcaacgt ccccgccggg
    15361 gctgttgccg cgttgcggat accgggcgaa ttcagcccgc tcgcgttgct gctcgacgcg
    15421 gccggcaacc cgtaccccgt gacgagcggc gtcgatgcgg agagcgaggc ggagatcaag
    15481 atgctgacgc cggaggctta caagtcggag acgttcttcg ccgtgcggcc cgaggattac
    15541 ggcgcgcagg ccgagaagct gagcttcgtg cagcgcgctc agggacgctt ccgctggact
    15601 ggctcgtggc tgtcggtggt gacggccgtc gatccggtgg actccttcga actctcgcca
    15661 aaaaaccgcg cgcaggtcga ggcgctgctg ggctgtcgcc gtcaggccgg gcgcgacgta
    15721 atcgtcagag acccgagatt cgtcagcgtc gatctcatca tccgcctctg cgtggcgcgc
    15781 tccgcctttg ccgctcaggt gcgcgcacgc gtcgtcgaag ctctcttcgg gaagggcggg
    15841 gcgcggcccc gcgcgggatt cttcgacccc gacaacttca cgttcggcac gccgctgcgg
    15901 cggagcgcgc tggaggcggc aatcgtcgcg gtcgagggcg tcgaggcggt gaccgggatt
    15961 caaatccgcg cccacggggt gacagacttc cgcgacttcg aggcgctggc tttcgaggtc
    16021 ggcgacgagg agttgatccg cgtcgagaac tcatcgcttc ggcccgagcg cgggtcggtg
    16081 agtctcattc tggagggcgg cgcatgagct gttgcgaaga ccccaaaggc tcgccgctgg
    16141 agaccatacc ggcggggctt tcctcgctcc cgcggcagtt gcgcggcttc gccgaggtgc
    16201 gccgcgacct gctccgcgcg ctgggacgcg agccggggcc gctctacggc tggcgtcctt
    16261 cgggcgacga cttcggtttg atgtggctgg agatgtgggc ctacgtctcc gacgtgctcg
    16321 gcttctacga cgagcgcgcg gccaacgaga cgtacatccg cacggccgtg cgtcgcccgt
    16381 cgttgcgccg catcgtcgag cttttgggct acacgccgag ttcgggcgtg gccgcgaagg
    16441 cgagggtggc ggcgctggcc gacggcgcgg tggcggtgac gctgccgccg gcgacgggct
    16501 ttcgttcgag cgccttcggc gcggagtcgc cgcaggtgtt cgagacgacg acggcgcaga
    16561 ccatccaccc gctcaagaac cagtggaagg tcacgagctt caaacgccgc cccgtggtgg
    16621 acacggcccc cgacgtgacc gaaacgagcg cgccgaccga tcagaaaaag gcggcccccg
    16681 cggtgggcac gccgaacgtg cgcgcccttc tctttgagac cgccgggttt gggctggcgg
    16741 cgggcgagat ggtcttgctt gacagccggc aggtggtcga ggagaacccg gtcgaacccc
    16801 cggtgtcgct cgtcaccgcc tccgagccgt tcgccggtaa ggactctgtg acctacatcc
    16861 gggcgacgct tgaacccggt gtccgcatcc cggccgacac cgatctctcg acgctccgtg
    16921 cgcggcgtcc gacgcagacc gcgacgccga ccgtcaatca gccggtgggg ccgggcggcg
    16981 gcaagggcaa gggcacgact ccggccgtgc ggaacgtctc gggcaagacg cacgtgttct
    17041 tcgaccagac gccgggcgcc ttccgcaagt cagaccccat tatcgtggcg cgcaacctcg
    17101 ggggcaggga agctgagtac cgattcgcga ccgtcgcctc cgcgcagccg gccgccgtca
    17161 agctcacgaa cattcccgaa cagaaggtgt cggttcccca gcagagcggc cctgacaaga
    17221 cggtgacgat cccgtcgccg acggtggccg ccaccgagtt ggtcatcgag ccggcgttgc
    17281 cgttgtcctt catcaaccat gcggatgagt tgaagttcct ccacaccttc gtcgaaggcg
    17341 gccagccgac gaacgtcggc aaaacggaag tgagcgccgc cgatctcgcc gaccccaagg
    17401 gcatcccgct cgaaggcatc gtcgagccgc cgcccgaagc ggcagccacc gccgccgcgc
    17461 agggtctcgc gcagaccagt aacgtgaccg gcatcctcga acaggagttt ctcatctccg
    17521 acgccgcgcg caacggcgtg gccgcgtcgg gccgcatcac cttcacggcg gatggccggg
    17581 cgagctttca ggcgctcgcc ctcgcgcagt tgccggcgtc gtctttgcgt ctgccgctga
    17641 ccatctacgg caacgtcgtc gagacgacgc gcggcgagag cgtcttcggc gagatactcg
    17701 gagccggcga cgcgcgcatc gccaaccaac gcttcaagct gcggaagaag tcgctcacct
    17761 acctgccgct cgccacgccg ggcggcaatg ccgcacaggt cagcacgctc caaatccgtg
    17821 tggacggcgt gctctggaaa caggtcaaca ctttcttcgg cgcggggccg gaggatgcgg
    17881 tctacacggt gcgacacgac gacgagctga acacctacgt caccttcggc gacggccgtc
    17941 gcggcgcgcg tctcccgtcg ggcgtgcaaa acgtcgtcgc cgactatcgt ttcggcgcgg
    18001 gcgcggccag cccgcccgcc ggctcgatca accagctcgc gggcgcggcc aagggcctgc
    18061 gcgcggtgcg cggccccgtg gccgccaccc ccggtaaaga ccccgaccag cccgaacatc
    18121 tacgcaccaa cgcgccccgc accgcgctgc tcttcgggcg cgcggtctcg gcggccgact
    18181 tcgaggcgtt ggcccgcgag caggcggggg tcgtgcgggc gaaggccgaa tggttgtgga
    18241 ttccgtcgca gatgcaggcg ggcgtcgtcg tccagtacat cggggaggcc ggctctgtgg
    18301 ccgaggcgct gcgcgcgcag gccgacccga ccatccccat cgaggtgacg aaggcgcaag
    18361 ccatcccgac gaccgtctcc atcggcgtcg aggtggacgc tcgttttgtg aaagagacgg
    18421 tggccgcggc cgtccaaaag aagttgacgg agccgggcac gggcgtgctc agccgcgagc
    18481 aggccggcat cggagggacg ttctggccga gcgttctcta cgaagccgtc gcgcaggtgg
    18541 agggcgtgct cgccgtcagc ggcgtcacct tctcgacccc gagcggcggg ccgacgatct
    18601 cgaactcaaa ggggacgtgt attgagacgg gcaagtatct cgactttacg ggtgagggcg
    18661 cggtgacggt gacgggcgtg gagccgctcg acagcaagcc gcggcccttc gccggcggga
    18721 cgactttgtg agtgacgcgg aatgagtgac gcgaaatgag tgacgcgaac cccgtgcgcg
    18781 acggctacga gagctactac acggagaagc tgtggcagct cgtgccggag gtctaccgcc
    18841 acgaggacgg gctggcggcc aaccccggcg tcctgcgtcg gctcgtcgag ctgatcggag
    18901 acgaggtcgc gcgcagccgc cgctcgattg accggctgtg ggaagaccag cacatcgaga
    18961 cctgtgacga ctgggccgtc ccatacatcg gcgatctggt cggcgcgcgt ctggtctcgg
    19021 ccaacgaccg gcgggcgcgg cgcgtggacg tggcgaaagc catcaacttc cgccgccggc
    19081 gcgggacgcc cgaccttctg gactcgctgg tgcgcgccat gtcgaactgg gacgtggtgc
    19141 tggtcgaagg tttccggcgg ctggcgcgca cacgccaccg gctcgacccc gtgccgcgcg
    19201 cgggtggctt cttcaccgga actctctcgg gcgggacggc cgacctgcgt agcccggcgg
    19261 gcgcggaagt gagcgacggc ccattcgacg aatacttcca cacgctcgac acgcgcctgc
    19321 tgcgcgggcg cgacgggcgc ttcggcatca acaagctcaa cttccatctc tatcgcctgc
    19381 gcgcttacga gatgacgaat gttgacccgg tgttgatgac cgatcccgcc gggccgggtt
    19441 tcccgcgcat ctacaccatc gacccgagcg ggcgcgacgt gcagctcttc atcgacgccg
    19501 ctccgcccga cccggacgcc gtcgccgccc tgccgggtca gctctcgcga cccgcgtgcg
    19561 gcctgccgcg cgagtgggag gtgacgcagg cgatgcgctg ccgcctgctc ggccacgtcg
    19621 cccacgtggt cacggcgagc cacgtcgaag agttgcgcca gctccccaac ccgccgggcg
    19681 cggccgacga gcaggcgctg tttcggattt tggaagtgcg cttcgagagc gagcgggcgt
    19741 tgcgtcgtcg cctgcgcgac ctcggcgcag gcatagccgt caacccgcct gactggtatc
    19801 gcgaactcgt cacgctcagt ctgcttgagc agaccggcaa ggcgaacctc tacccggcgc
    19861 aggtggagat ttcggccgtg ggagtttcgg gacggttcga gccgttcgcc ggcgtcaggg
    19921 tcgccgccgc caacctcgcg agccgcgagt gccaacccga cccggagaac aatctcgcca
    19981 cgctcctcat cagccccgaa cagggacgct tcgccagcgt cccgcccgac gaccccgcga
    20041 acttcgagcc gttcgttgaa cgctaccact acggcttctc cggcgacgtg ggggccggct
    20101 cctatccgcg cgaccccatc ccggcggccg acgttccgtc gcgcgtcgcg gtggacggtg
    20161 tcgtccccgg cggcggggtc ttgcagggcg acggtttgct cgtgagcgac aaccggactt
    20221 actcgctctc catcgtcgcc gccacaccca tcgacaacgc cgtccttcag gcggggccga
    20281 agctgcggcc ctacgtcgtg ctgagcgacg gcggcgcgaa cctgcgaccg ctggctatca
    20341 acacgacctt cctcatcgac ggcggttggt acggcgcggg cgacccgtcg aatcctccgg
    20401 cggcgggcgc ggtcgccgat ttcgtcatcg aaggcgcggc cagcgcagcc accgaggcgt
    20461 tcgacttcga gcgcgtcgaa attcgcttcg ccaccctcga ccccggcggc gtgcgcgccg
    20521 acggcgtgcg gattccgccc cttcgcctgc tggtgcgcgc gcgcgtccgg cagttaatca
    20581 tccgccgctc gatcacgggg ccgattcacg tcgtgcgcga taacccggac gacccttcga
    20641 tggtcgaaga gttgttgatc tgcgagtcaa tcgttgacgc gcgcgaaacg attgaccgcc
    20701 tcgccgtgtc gagcaacttc gggacggtcg tcatcgaagg ctcgaccatc ttcggcgacg
    20761 tggcggcgga cgtgctcaag gcgtctgact cgattgtcga tgggcaggtg cgggtggtga
    20821 acaatcaggc cggctgcttc cgcttcagcg ccagcgagcc gggcgacgat gtaagacttc
    20881 cgccgcgcta ccgcgacgcg ttcgcgccca tcccggagtc ggctttcaac tcgacgacct
    20941 tcggcgatgc gcagtacgcg cagttgtccg tcgtggcccc gcccgcgctt ctgcggggcg
    21001 cggagaacgg ctcggagatg ggcgtcttct cacacctgct gtcgccgatt cgtctcgcga
    21061 gcgtgcgcgc gaaggtcaac gagttcggcc ccgtcggatt gctcgcgcag tatctcttcg
    21121 agggcgacta ccgggcgcag tcgctcgtcg agttcggcgt cttgaatccg acgcctgaag
    21181 agtcgccgcc gcccccgccg cctgaccccg gcgatgtgat cccggagccg ccgcccgagg
    21241 aggaagagcc tctgccgacg acgtgccccg acgaagagcc gccgacgggg gaggagccgc
    21301 cgccgcagat cgccgagttg accatcgtga acatcaacgg cgcgccggag ttctggcgcg
    21361 atgtcgattg gcgcgaggag gtcttcccgg ccccgaaccc caacggcgtg aggcgtgaag
    21421 gcgttccgtt cgaccgcgtg cgggtgctcc tcgaatacga cgcgcgcttc gggacgccgc
    21481 ccgacgagca gggctggacg ccgaacaacg aggaggccgc cgcgctctac acgctcgacg
    21541 ggcggggagg tctggcgttt cgcatcgaca accgcccggc gttcttcacc gcgcaggccg
    21601 agctggatga ggagctgccg gctcagatgc acgcttacgc cagcgtcttc cttgacgaag
    21661 tcccggaagc ccgcgcagtg cctttcgacg gcttcgagca acgcgtcgaa gtgaatgacg
    21721 ataagcgacc ctctcgcggc atgcgcgccg attggttgag gacttcggag aggacgacgg
    21781 atgggcgcga gctgcactat ctcacgctgg acgggcggga gattctcggc tcgccgaagc
    21841 ccgcgcgcga agcgcgtggc tggcacgagc tgtctatgca gtgttgcttc agcgacgacg
    21901 acaagggcgg ttgtttgtcc ggtctgataa agatgaagct gccgaacgac gcgaaggcga
    21961 acgcggaagc gcgcgacgcg aaggcgaatg ccgaggacga cgttgccgtg aacgccgccc
    22021 gccgtatcga gggcgacggc cgggccttgc tttccttcga cgctcacgtc gatgaactca
    22081 agctccgcaa gttcggtgaa tcacgcggcg acttggggcg cgcgagcctg cgggccgtct
    22141 tcgggttcga gggaaagacg ggtcagatgg ccgggcggct ccgcaacttc gtcgtctccg
    22201 cgccgggccg ctacgtccgt gcgtggatga cgggcaacac gccgtcacgc gcgcccatgc
    22261 tgcggctcgc cttcgtcacg caggagcgcg tgacggcggg cgttcgtttc cgagttcgct
    22321 acgggcgaca gaagaaaaac gatccgacgc ttcctctgcg cgttgccgaa gcgactctca
    22381 agctcgtaga cacagagccg gggcgcgaga cgctgctgga gattcccctg ccggcggtgc
    22441 gcgccaaaga cacagtcgtg ttgacgcttg agcgtgattc gagccacacg gacgacacgt
    22501 tggagacgag tattcgcatg atgagcgcgg cgctggtcgc ccgctaaagt cggccgaaga
    22561 aggagactat gccgaacgca gacatcagcc gttacctgaa tcagccgggc aagcactaca
    22621 agggcgcccg cctgcaacag gggcgcgcgc tgctcgactc ggacttcaat gaaggggccg
    22681 aggcgcgcag cgacgatctg cgcgcgtcgc tcacggacat catcggcgcg gcggcgagtc
    22741 gcgacgacgg cttcatgccg gccctgaagg tcggcgacgt ggtcaactcg aagctcgtgc
    22801 gcttcggcgt gacgacgcag gcatacgtgc tcgactaccc tttaaagccc ggctcgatgt
    22861 acgccggcgg cgcgctctgg gaacagtccg aggcggagcc ggtcgtcttc cagcgcgagt
    22921 ttttgcagat gggcgcgggc gtggccccgc gcgccgcact gggcacacag cgtcagctct
    22981 cctacctgcg cggatgggag cagagcgtga cggcggtcga ggacggcgag ctacaggagc
    23041 cttcgctggg cggggccgac gccgcggcgc gtgtccgccg cgtccggcgg gtggaagttc
    23101 gcaacgtgca ggcgacggag tgccgggcgg cgttcgatga agtgctcgcc gacctcggcg
    23161 acggcgacac ggccacctac gatgcggcga cctgcgagct gcgctcgaac gcgcggctgc
    23221 ggatgacgtt tcagggcgcg cccgatgtcg aatgcggccc gtgccacccc tcgctcgaag
    23281 gccgctacct cggcggcgag ggccacgcca tccgcatcat gctcaccgcg ccggatcgct
    23341 tcgtgtgggc cttcgacaac gcggccccgc tctaccgcgt ccggctcgtg ctcgacggcg
    23401 ggggcggcgc gcgtgtcgag atgcagacgc cgccgaagga cgcctatcac cacccgcggc
    23461 agaacatggt ggtcgagttc ctgccgtggg aggctctgct ggaaaacggc aagccgcagg
    23521 gcggcaagat tgcgggcgag accgtccgca acgagcgcat cgccgcgcgc gtcggactcc
    23581 tcgctgagac ggacggcccc tacgacgccg cggcgcgtac tttccacgtg cggttcgcgc
    23641 ccggcagcgc cccgcagacc ggcgtcggca aggggcagac aaactccaaa gcgcagtcgc
    23701 agccggccgc gaacgtgaag accggagacg gcccctcgaa cgacggcgtc gcgctgcgct
    23761 gggacgcgca gcatccgttc gacgagcagt tgaacccgac cgacaccagc cccgacgggc
    23821 ttgtcacata catgtacatg cgcgtctggc atctgaagtc ggacgccgcg ctcaccattc
    23881 caaccacatc gaccagaccg ctcggacaga ccgggctggt gccggtcttc accggccgtg
    23941 gtcgtcacgg tgacttctgg actatcaccg tccggcccga agcgcccgac gaaatcctgc
    24001 cgcgcgagat catgctgccg ggtggtcggc cgccgcacgg gccgcgcgaa gtagtcacgc
    24061 aaatcagtct cgtgacgtgg gtctcgctca ccggctccgt tcacaaggtg gtctccatcg
    24121 aagactgccg gcccacgctg ccggccctca ccgagcgcgg ctgctgcaca tacgacgtag
    24181 gccccggcgg cgacttcgag accattcagg cggccgtgag cgccctgccc gtgagtggcg
    24241 ggcgcatctg cgtgaggccg ggcgtctacc gggaagagat tcgcatcgcg ggcaaaagca
    24301 acgtcgtcgt gacgggctgc ggcggcgctc gcacgcgcgt cgtgtcgccg gagacggcca
    24361 cgggcgatgc gctcatcgac atcgacctcg cgggcgacga gcgcggcgtc acgctgcgcg
    24421 atctggcgat tgacgcgagc gggcagatcg gcgtccgcgt cgcgggcggg agccgcgtcg
    24481 aactccgccg gttggagttc gcgcaggcgg cgggcgaggg gcagggcagg gtgacggggc
    24541 tgcggagcgc cattcagatc atcggcacga cggacgtgcg gatcacacgc tgccgcatgg
    24601 agcttgccgg cacgttcagc cagcacgccg ccgtctacat cgacacgccg ggcggcgcgc
    24661 tcgtcgaagg ttgtcacatc gagacgcgcg ccgatcaggc gggcggcgtc agcttcgcgt
    24721 ggggcggcat cgaggtggcg ggcgcttcgc gcgatgtcga gattcgcgac aacgtcatcc
    24781 gcggcggacg cggccacggc gtcacgctcg gcggagtggt ctaccgcgcg ctcgacggcg
    24841 tccgcctcgg cgtcgaaggc gcggggcgcg ggcagtcgaa tccggccccg ccgttcgccg
    24901 tccgcggaac cttcgccgtg gtcgaggcca acccggacgg tcggggccgg agggagtttt
    24961 tccccgagcc gcaggcggcc atcgacgagt tgatcatcgc gggtaatcac atcgaagccg
    25021 tcggcggcag cggcatcgcg tctccggcgc tccgggtcga gcacgacgca cgcctgaccc
    25081 ggccgccgct gtgcttccga cgcacgacgt tcgccgtgac gaacgccgtc atcgaagaca
    25141 accgcatcct cgacaacgcg cgccagcgca cggccatcgg cggcgagcgc gaggcgctgg
    25201 gcggcatcgt gctttcggac gcgcgtcgga cgacggtgcg caataacctc gtcgaaggga
    25261 acggggcggg gacgggcgcg cccgtgtgcg gcatctgcgt ggcgcacggc gagcacgtct
    25321 ctttagtctc gaaccgcgtg ctgggcaacg ggacgaggac gccaaacgcg ccgcccgccc
    25381 tcactcgcgg ccagaggtat cacggcggca tcgtcgtcgc ggcccccctg tcggctaccc
    25441 agacgaactt cgtcgatgag gccgcgccgc gcaacgtcta ccttcggcgc aacgtcgtcg
    25501 agcagcccga aaccgtgtcc gtcttcgtcg cctcgcgcgg cgcttgccgc atcacgaaca
    25561 accacttcca ttcgcacgtc cgcgaaggga gcttggcggt gccgacggtg ctcgtcttct
    25621 cttcggggaa gccgtgggag gccgtcgatc tgccggaggg cgagccgaac ccggcccgct
    25681 ggcgccagcc cgacggctcg cgcgaatacc tgaacggccg cgcgcaggat ttgcccgacg
    25741 gcgacggcgg cgcgctctgc ttcagcggca atcaggtcat cacgaacggc gcgagcgaca
    25801 tgggcagcag cttcagcggc ttcggcgccc tcctcgtctc gttcgatcac gtctccgcgg
    25861 tcggcaacca gttcgccgca cggagccccg tgcgggcgcc gcagcggcag ccgccgcacg
    25921 tcatggtcat cggactgacg gcggacgcct ccgccaaccg cgtcgccgag ggagtggagc
    25981 agaccaattt ctccctcgcg gcgatgggcg cgatgatgac ggcgggcgcg ggcaacatcc
    26041 tcacgcactg cccggccgtc ttcggttgcg aaaaccacaa cgaccgtgac tacttcgtgg
    26101 cggaagacaa cctctcctgg ttccggccgg cgaccgccgg gtgcgggccg ctgacggggc
    26161 cgttgttcga aatcctgaga atcctgtgca acgccctctt cggacgcgcc cgcggcgtca
    26221 accgtaatcc caacatcgtt cgcgacgtgc agctaattcg cgcattggag cgcagctagt
    26281 tcgcggtgta gagctatgac gacaaacgcc gacatcaccc gcctcctcat ccagccgaga
    26341 aagcactacg tcggcgcgcg catgcaacag ggccgcgtcg tgctcgactc ggacttcaac
    26401 gaagaggccg cgctcggcga cgaagaacgc cggcgcgccc tgctcgacat cgtcggcccg
    26461 acgggcagcc ccgacgacgg cttcgcggcc gagctggaga tcggcgatca ggtcacgatt
    26521 caacccgtct cctttggcgg cgcgcccgcc gtcaacacgc tcaactatcg tctccgcccc
    26581 ggcgtgatgt atctcgcggg cctgcgcttc gagcacgaag aacgcaccat caacaacgtg
    26641 gccggcggcg accccatcgt cttccagcgc gacttcctcc agatgcgcga cgccgacgcg
    26701 ccgcagacgg cggaggtcgc gcacgcgcag ctcacctacc tgcacgcgtg gcagcagtgg
    26761 gtaagcgcga ccgaggatga ggagatacgc gagcgcgcgc tcggccgtct cgacacctcc
    26821 gtgcgtgtgc ggcagatggc gcgggtggaa gtgcgcgagg cggcgaacac ggtggactgc
    26881 gcgggcgcat gggccgaggt gcgccagcag atcgaggccg aagccggggg cgctttcgac
    26941 gcctcgggcc acgagctttt gtcgccggcc cgcctgcgcg tgacgttcgt gccgggcgag
    27001 gcgctcgaca cctgcgcgcc gtgtgcgccg gacgacgtgg gccgctatct cggggccgac
    27061 aatcaggcga ttcgcatcat gctcgcggcc gccgaccgtt acgtctgggg cttcgacaac
    27121 gcctcgccgc tctaccgggt cgaactcggc gagaccgagg acgggcttgt tcccgtgcgg
    27181 atgattaccg agccgcgcga cgaggcgcgc tggccgctcg tcaacacggt ggtcgagttc
    27241 ctgccgtggt cggccctgct cgacaacggc gaaaaggtcg ccgccgaaac cggcgtcttc
    27301 ctccgcgtcg cggaaggctt cgaccccgac gtgacctcgt tccgcatcga cgcggccgac
    27361 ctcccgcaac tcgacgacct cgtccgcgac tgggacgccg cgcacccgga cctttcgttg
    27421 ctgcccaacg acgccgaccc cgacggccgc tatctctaca tgcgcgtctg gcatcgtctc
    27481 gacgccgaga ccgacgacgt gctgctcgac acggcggccg cgcccggcaa ccacacactg
    27541 ctccgccggc aaggtctgct cccggagttt accgggggcg ggcgcgtggg cgactactgg
    27601 gtcgtcagcg tccggccgaa caccccgcaa cagattacgc cgtggaacct gacgcagggc
    27661 ggcggcgctc cgccgcacgg gccgcgccac ctctacacgc cgctcacgct catcaacttc
    27721 cggccgccgg gggcgggcga gctggagaac acggaggtcg tcgaatccgt ccacgactgc
    27781 cggagaatct tccggccgct cgttgaccgg atgggctgct gcacgcacac ggtcggcgac
    27841 ggcgtcgatt cgcagggcga ctacctctcg attcagaccg ccatcgacgg cctgcccccg
    27901 gagggcggca aagtctgcct gctgccgggg acgtataccg aggcggtgcg aatcgagcgc
    27961 ggcgacgtga tcgtcgaagg ctgcggccgg cagacgcgct ggcgcactcc cgcgggcgat
    28021 cagaacccgg ccttgattca cgttctcggg ccgcgcgtca ccttgcgcga gctcgcttta
    28081 gaaaccttcg ggcaaatcgg cgtgctggcc gggccggagg atttgaactc cgacgtgttc
    28141 gcgaacgacc tcgtgttgga acgtctcgac atcgtcgccg acgaacgcgc cggcgtcggc
    28201 ggtcaggcgc gctgcgccat cgacgtgcgc cgcgggcggc gtgtgctcgt gcgcgagtgc
    28261 gaagtgacga tggaaggctc cgtgagcgac gaggcgggcg tcttcgtgcg gggcgaggac
    28321 atacgcgtcg agggctgccg catcgagacg gcgcgcgggg acgacggttt gccgacctgc
    28381 tggggcggcc tacaagtcgg cggcgcgacg cgccgcgtgc aaatcctccg caaccgtatc
    28441 cgtgaaggac tcggccacgg catcacgctc ggcagtctgg aatgggtggc cgaagagacg
    28501 gcgccgacgg gggggagagg ggaggtggat gatgaagcga cgcgggtgag gagggtggcc
    28561 agcttcggcg cgggcacggg cttgcagaac accttcgacc cctgcgcgcc gcagcaggat
    28621 tcggtgcagc cggtggaggt cgatcaagtg cgtttcgacc ccgagacggc cggcgacctc
    28681 gaagacattc acatcgccga caaccgcatc gaggcgatgc agggcaacgg catctcggtg
    28741 ctcacgaccc ttcgcctcgc cgacgacggc gagcaggatt tgattacggt tgaccgcctc
    28801 accatcgagc gcaatcgcat ctcgggctgc gtggtgcagt cgcgcacgct cgcctcgaac
    28861 tcgcccgtgc agaagacgaa gaagctctcg ccgggcgagg aagaagtttt cggacagttc
    28921 ctcgtctcca acatcccgcc ggccgggatc gccttagtcg atggcgagca catcgtcatt
    28981 cgctcgaacg agattcgcga caacggcacg accgacccga accccgtcgc cggcgtctgc
    29041 atcatcttcg gcaacgacat tttgattgag gagaatcgca tccagaacaa cgggctgcgg
    29101 cggccggcgt ccatcccttc catctccagc atgcgcgccg gcatcgccgt ctcgctggcg
    29161 ggcgtggcgt cgaacacgga gacgcagaat cagtcggaca cactgggcag ttcgctacgc
    29221 atcatcggca acatcgtcga acacccgaac ggcccggcgc tcgccgcgcg cgccaccggc
    29281 cccgtcgtca tcgaggacaa ctacctgctc tcgctcggca acaacgccac ggcgcagcag
    29341 gcgagcacgg cgcacgcggt ggcggtggtc aacgccggga tgccgtggga gtcgattgac
    29401 ctgcccgcgg gcgagccgtc gcccaaccgc tgggtcttcc cgccgggcac gcctgagtat
    29461 ttagcacgca agcccggcga cgaaggcggt gacggcggcg tcggcgcgcc cggcgaaggc
    29521 ggcggcatca tcgggcaggg cggccccgtc ctgttcagca acaaccacgt cacgctacgc
    29581 tgggtggagg cgagcaacgc cgccgccggt ctcgcctccg gcttctcggt gggcatctgt
    29641 agcctcgacg acgtgaacat gcacggcaac cagttcgccc tcaacgtcga agaccccgga
    29701 ctcaaaaaga aggacggcac gaccttcaat cgccagccgc gaatttccgc tcacgtcgtc
    29761 gcggtcgggg cgacggccaa cgcctcgcac aaccgcgtcg ccgagggcgt gaacgacgcg
    29821 ctgatctcgc tgcaagtcct cggcggcttc ctcgtcaacg cgagctacaa catcacgacg
    29881 cacatgagtt ttgcggcgac ctgccacggc atacgcgggg aggagggtgg gccggcgaac
    29941 ggcaaagacg agtaccgcgt ctaccgcggc aacctcgtct ggctgcgacc ggcattagat
    30001 gagagccgcg aagacatggt ctcgctcgaa acggtcatgc gggtctccca acagttgttc
    30061 gtcacgctgt gcgacaccgt cctcgggctt gggcctgacg acgacagtac gcgtttcata
    30121 gccatcctcg gaatcttcgc ggcgcggggg tagccgtgaa atggagggag cactgagagt
    30181 gagcgaggac gttttgagtg tgagcgaaga cgccccgcga gtgaaccggg cggcggtcgt
    30241 cgccggcgtg gcgcgcgacc tcgacgcgca cctgcgttcg ctgcggcgaa aactggaacg
    30301 ggccgccctg cgcggcgagc cggtcgaacg cttgcaggcc cgcatccggc gcgaagagga
    30361 acaactccgc cggctgcgcg cctacgaaca gggccagcta gacacgagga ggccggaatg
    30421 aaccgagggg ccgccatccc gaacaaaaga atcatcacct cgacggaaaa gcggcaggac
    30481 actccggccg cacggccgcc gacagcgcac gctctccggc gttcggccgc gagggtcgcg
    30541 actaccgcaa ctgcggcaag cgccgccgag ccgggcggcg agacgagcgg cgcggacacg
    30601 ggcttgtcgg cgcgcgtggc gacggccgcc gatttgccgt ggctccagac gtgggcggcc
    30661 cagctcggac tgccggcccc gaggtcgcgc cgggtgcgct ctttcgtctt gctcaaggac
    30721 gggcagcgcg tcggctacat ggcggcgcgc gacgagatgg ccgacgccgg gcgcgcgcgc
    30781 gagtcgatgc tctggatcgt ctcggcgttt ctcatcccga cgctgcgcgg gcagggcatg
    30841 attctgaagt tcggtgaaat cctctcgcgc gtgcattacc gcaaaggtaa ggtcgggtgc
    30901 cgggtcgccg ccgacaacac gccgatgatg aggctcatgg cgcgcggcgg ctggaagaag
    30961 ctccactcga cgcggcggtt caccgacttc atgttggaac tcgacgcccc tttccgggcg
    31021 tcgcgcaaag gctagcgagg tagagcgatg ccaagggcgg cagctccgaa agcgaaacga
    31081 gcggccaaac acggccagag cgtcggcgga gactcgaaga cggcgcagcg taaacgcacg
    31141 cgtcgccccg ccggcgcggc ctctcccgcg gttggcgtcc tccaaccgaa gctgaaagtc
    31201 agcgccccgg acgaccagtt cgagcgcgag gccgaccacg tctccgaccg cgtgatgagc
    31261 atgcccgcgc ccgctggcgc ggcgggcgcg actgtcgggc gcgaccagaa ggaagaagag
    31321 ggcaagagcg tccagcgaaa ggaggcggaa gaagaaaagc aggcgcagac cttctctttg
    31381 caacgcgaag agaaagagga agagaccact tccgtgcagc gacaggccgc ggaggaggag
    31441 ccgaccgagg aagtggcgca gaccttctca ctccagcgcc gtgaagacga agaggagcaa
    31501 acgtcttctg tccagcgaga ggaaaaagaa gaagaggaga agtcatcatc cctgcaacgt
    31561 aaggaggaag aagagcagtc gtcatctctt cagcggcgcg ccgccgggga ggcgaaggag
    31621 cagatgcagc agcgggccaa acgcccgcga cggcccgaaa tcactccaca gttcgagacc
    31681 gacctccgac tgctgcgccg cgccggcggg cagcctttgc ccggccacct gcgcgccttc
    31741 cttgagccgc gcttcg
//
LOCUS       DASVES010000449         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1055028, whole genome shotgun sequence.
ACCESSION   DASVES010000449 DASVES010000000
VERSION     DASVES010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055028"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VF240_18670"
     CDS             <1..1396
                     /locus_tag="VF240_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177288.1"
                     /translation="WDRLASGQPPALLYWYRQSPRYLQPYNARAVTTADPPRVVSGMA
                     FVTLDTRGRLVSFEGVPPHLNEPQTQAATAAFDWSLLFNEAGLDITKFTPAEPKWLPP
                     QPYDTLAAWEGAYPDQSDMTMQVVAATFRGRAIYFHIIDPWNRASRQVAARQDAQAKV
                     LQAVLIVFLLLILSSAVFLAWRNLRLGRGDRKGAFRIAFVVFVLYMIAWLFSAHHVPD
                     LGEFALFIENLAWSLMIACMMWIVYIALEPFVRRRWPRRIISWSRLLAGDFRDPLVGR
                     DILLGAVFSFGLLLIGYLVEYVPRLLGQPPSTPSIHGLALNALQGTQQLIPTFTAQVI
                     NSFVTPAGMMFLLLLFSIILRREWIGVGLLWLIFTVIGALTGEHPAIDWAFAAASSAA
                     MLFILLRYGLLTMVAMQFFVLGFVFYPITTDFSAWYAGSTIFAIAVAVALMAYGFYIS
                     LAGQKLFSGDLIGE"
     gene            complement(1499..>2983)
                     /locus_tag="VF240_18675"
     CDS             complement(1499..>2983)
                     /locus_tag="VF240_18675"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEX7177289.1"
                     /translation="VVSYAQNQRTAWWGEDENLVRWGHLDTFAAAPTFAFMVSPKQAR
                     ALQARLARGEQIRLRATVRAGKHAGAYTVVTATIPGADPRLRNEEIVYSCHLDHQRPG
                     ANDNASGSVAILEVARTLQKLIREGRIAPPRRTLRFVWPPEIEGTITYLNARPEIASR
                     AKAAIHMDMVGGGPETKAVFHVTRGPASLPSFVYDVAEHFGAFASEQSDKFAAGDATD
                     YSLHAPEGGREPLQAALVEFSMGSDHQVYTDSSFGVPSVYLNDWPDRYIHTNFDTPAN
                     VDPTKLKRAAFIGAAVGYFLANTGPADAPALLALLQQNGLRRAARMLERRAALADAAE
                     AANLTRFHLRHERETLTSMERFFKVPEGVDAQAAAYLDNLQRLVGETRTVAPAVAATT
                     TGGSIVFRRNPSVRGPMSAFGYDYFTDHYGEERAAKIRLLNFQGLRGGGGEYAYEVLN
                     FADGRRTAREIRDAVSAVYGPVPLEVVVEYLRALESIKVVSSKQ"
BASE COUNT          433 a         1004 c          981 g          565 t
ORIGIN      
        1 ctgggatcgt ctcgcctcgg ggcagccgcc cgcgctgctc tactggtaca ggcaaagccc
       61 gcgctatctc cagccgtaca acgcgcgggc tgtgacgacc gccgaccccc cgcgcgtggt
      121 ctccggcatg gccttcgtca cactcgatac gcggggccgt ctcgtctcct tcgagggcgt
      181 cccgccgcac ctcaacgagc cgcaaacgca agcggccaca gcggccttcg actggtcgct
      241 cctgttcaac gaggccggac tcgacatcac gaagttcacc ccggccgagc cgaagtggct
      301 gccgccgcag ccctacgaca cgctcgccgc gtgggagggc gcttaccccg accagtctga
      361 catgacgatg caggtcgtcg ccgccacctt ccgcggccgc gccatctact tccacatcat
      421 cgacccgtgg aaccgggcat cgcgccaagt ggcggcgcgt caggacgcac aggcgaaagt
      481 ccttcaggcg gtgcttatcg tcttcctgct gctgattttg tcgagcgccg tctttctcgc
      541 gtggcgcaac ctgcggctcg ggcgcggcga ccgcaagggc gctttccgca tcgccttcgt
      601 cgtcttcgtg ctctacatga tcgcctggct cttctcggct caccacgtcc ccgacctcgg
      661 ggagttcgct ctctttatcg agaacctcgc gtggtcgctg atgatcgcct gcatgatgtg
      721 gatcgtctac atcgcgctgg agccgttcgt ccgacgacgc tggccccggc gcatcatctc
      781 ctggtcaaga cttctcgccg gcgacttccg cgacccgctc gtcgggcgcg acatcctgct
      841 cggcgccgtc ttctccttcg ggcttttgct catcggctac ctcgtcgagt acgtgccgcg
      901 cctcctgggc cagccgcctt cgacgcccag cattcacggc ctcgccctca acgcccttca
      961 gggcacgcag cagttgatcc cgaccttcac cgcgcaggtc atcaactcgt tcgtcacgcc
     1021 cgccgggatg atgttcctgc tcctgctctt ctcgatcatc ctgcgccggg agtggatcgg
     1081 ggtcgggctg ttgtggctga tcttcacggt catcggcgcg ctcacgggcg agcacccggc
     1141 gatagactgg gcgttcgccg cggcgagttc cgccgcgatg ctcttcatcc tgctgcgcta
     1201 cgggctgctg acgatggtcg ccatgcagtt cttcgtcctc ggctttgtct tctaccccat
     1261 caccacggac ttctccgcct ggtacgccgg ctccaccatc ttcgccatcg ctgtcgccgt
     1321 cgccctcatg gcctatggct tctacatctc gctcgccggg cagaaactct tcagcggcga
     1381 cctgattggg gagtgagcgg agagcagtaa gcagtgagca gtaagcggtg agcagtgagc
     1441 agtaggatac ttgcgaggct tgccgcgcac ctccgaaact ttttttactg ctcactgctc
     1501 actgcttact gctcactacc ttgatcgatt ccagcgcgcg caggtattcc acgaccactt
     1561 cgagcggcac ggggccgtag acggccgaga cggcgtcgcg tatctcacgg gcggtgcggc
     1621 ggccgtcggc gaagttcaag acctcgtagg cgtactcgcc gccgccgccg cgcaacccct
     1681 gaaagttcag cagccgaatc ttcgcggcgc gctcctcgcc gtagtgatcc gtgaagtagt
     1741 cgtagccgaa ggccgacatg gggccgcgca cagatgggtt gcggcggaaa actattgagc
     1801 cgccggtcgt cgtcgccgcg acggccgggg cgaccgttcg cgtctcgccg acgagccttt
     1861 gcaggttgtc gagataagca gccgcctgcg cgtccacccc ttcggggact ttgaagaatc
     1921 gctccatcga ggtaagcgtc tcgcgctcgt gccggaggtg gaagcgcgtg aggtttgcgg
     1981 cctcggcggc gtcggcgagc gcggcgcggc gctccagcat ccgggccgcg cgccgcagac
     2041 cgttttgttg gagcagcgcg agcagggcgg gcgcgtccgc cggccccgtg ttcgcgagaa
     2101 agtagccgac cgccgcgccg atgaaggcgg cgcgcttgag tttggtcgga tcgacgttgg
     2161 cgggcgtgtc gaagttggtg tggatgtagc ggtcgggcca gtcgttgagg tagacggaag
     2221 ggacgccgaa agacgagtcg gtgtagacct gatgatcgct gcccatcgag aactcgacga
     2281 gcgcggcctg caaaggctcc ctgccgccct cgggcgcgtg caggctgtag tccgtcgcgt
     2341 cgccggcggc gaacttgtcc gactgttcac tggcgaacgc gccgaagtgc tcggccacgt
     2401 cgtagacgaa cgaggggagc gaggccgggc cgcgcgtgac gtgaaagacc gctttcgtct
     2461 cggggccgcc ccccaccatg tccatgtgaa tcgcggcctt cgcgcgcgag gcgatttcgg
     2521 gacgggcgtt gaggtaagtg atcgtaccct cgatctccgg cggccagacg aagcgcagcg
     2581 tgcggcgcgg cggcgcgatg cgtccctcgc ggatgagctt ctggagcgtg cgcgcgactt
     2641 cgaggatggc gacgctgccc gaggcgttgt cattggcgcc gggccgctgg tggtcgaggt
     2701 ggcaactgta gacgatctct tcgtttctca ggcgcggatc ggcgccgggt atggtcgccg
     2761 tgacgacggt gtaagcgccc gcgtgtttgc cggcgcggac ggtggcgcgc agtcgaattt
     2821 gttcgccgcg cgcaagccgc gcctggagcg cgcgcgcctg cttcggcgag accatgaagg
     2881 cgaaggtggg cgcggcggcg aaggtgtcga ggtggcccca gcgcacgagg ttctcatctt
     2941 cgccccacca cgcggtgcgt tgattctgcg cgtaactgac gac
//
LOCUS       DASVES010000450         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_459193, whole genome shotgun sequence.
ACCESSION   DASVES010000450 DASVES010000000
VERSION     DASVES010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459193"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="xseA"
                     /locus_tag="VF240_18680"
     CDS             complement(<1..684)
                     /gene="xseA"
                     /locus_tag="VF240_18680"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7177290.1"
                     /translation="MTRSLLLSLFDEVERRPLSVSELTSQVRGALENRFASVWVEGEI
                     SNFKAHASGHWYFTVKDESAQLRATCFRGVNHRIRFRPSNGLQVRARGRLTVYEPRGD
                     YELLVEGLDPVGAGALRVAFEQLRDRLAAEGLFDAELKRELPLLPRRVGVVTSPTGAA
                     VRDIVRTIERRTRTVNVLLAPVRVQGEGAAREVAQAIALLNEFHRRACAEGRAHEGLD
                     AIIVGRGGGS"
     gene            complement(681..1022)
                     /locus_tag="VF240_18685"
     CDS             complement(681..1022)
                     /locus_tag="VF240_18685"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX7177291.1"
                     /translation="MKLLLDECVTRYLKPEFTGHDVYTVDEAGLKGLKNGNLLRAAAG
                     KFDVLITVDKSLSYQQNLSLYSLAILVLVARSNAFAALKPLIPQALRALDRVEPGQVV
                     RVEASREGDTL"
     gene            complement(1006..1230)
                     /locus_tag="VF240_18690"
     CDS             complement(1006..1230)
                     /locus_tag="VF240_18690"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX7177292.1"
                     /translation="MNKTQLIERDPEKLGGTPVFYGTRVPIKNLFDCLEAGERLNEFL
                     DQFPTVTREQALKVLEASRETLLADDEVAA"
     gene            complement(1312..>2310)
                     /locus_tag="VF240_18695"
     CDS             complement(1312..>2310)
                     /locus_tag="VF240_18695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7177293.1"
                     /translation="PTPTPSPTEAPDDEVERVDVDLASVLLTAVDRERRFVTTLRRED
                     IRLFEDGVEQQITLFQRETDLPVSLVVLVDTSASQEAVMEDEKSAARAFIDSVLRPER
                     DTAAVLSFTGIIRFEQPATSAKADLGGAIDRLKVLYSSDSPECNDENDNIPEEQILRC
                     KTAVWDALWLSVEEVLSKTPERTRRAVILLSDGDDTVSRRERQEAADFAVKNNTVIYA
                     VGIRDEEFPHGKLQRGDLRKVSERTGGRAFFPTNRAELDAAFAQINDELRAQYLVAYT
                     PANRQRDGRFRRVRVEITNPALRKAKLQLLYREGYYARGASAAPAPVTKGDSGRKN"
BASE COUNT          373 a          718 c          820 g          399 t
ORIGIN      
        1 ggagccgccg ccgcgcccga cgatgatggc gtcaagtccc tcgtgcgcgc gcccctcggc
       61 gcacgcccgc cggtgaaact cgtttaagag ggcgatggcc tgcgccacct cgcgggccgc
      121 tccctcgccc tgcacgcgca cgggcgctaa gagcacgttg acggtgcgcg tccgccgctc
      181 aatggtgcgc acgatgtcgc gcacggccgc gcccgtgggc gaagtgacga cgccgacacg
      241 acgcggcagc agcggcagct cgcgctttag ttctgcgtcg aacaaccctt cggcggcgag
      301 ccggtcgcgc aactgctcga aggcgacgcg cagggcgccc gcgccgacgg ggtctaagcc
      361 ctcgaccagc agctcgtagt cgccgcgcgg ctcgtagacg gtgagccggc cgcgcgcgcg
      421 cacctgaagg ccgttcgagg ggcggaagcg gatgcggtgg ttgacgcccc gaaagcacgt
      481 cgcgcgcagt tgcgccgact catccttgac cgtgaagtac cagtggccgg aggcgtgcgc
      541 cttgaagttg gaaatctcgc cctcgaccca cacggacgcg aaacgattct ccagcgcgcc
      601 gcgcacctgc gaggtcagct ccgagaccga cagcggccgg cgctcgactt cgtcgaacag
      661 cgagagcagc agggagcggg tcatagcgtg tcgccttccc gcgaagcctc gacgcgcacg
      721 acctgccccg gctctacacg gtcgagggcg cggagcgcct gcgggattaa cggcttgagg
      781 gcggcgaagg cgttgctcct cgcgaccaga actaaaatcg cgaggctgta aagcgaaagg
      841 ttttgctggt aagacagact tttatcgacg gtgatgagca catcgaactt gccggcggcg
      901 gcgcgcaaaa gattcccgtt cttcaggccc ttgagccccg cctcgtcaac ggtataaacg
      961 tcgtgcccgg tgaattcggg cttcaggtag cgcgtcacac actcgtcaag cagcaacttc
     1021 atcgtcagcc agcagcgtct cccgcgacgc ctcaagcacc ttgagcgcct gctcgcgcgt
     1081 cacggtcggg aattgatcga ggaactcgtt caggcgctcg cccgcttcaa gacagtcgaa
     1141 tagatttttg atcggcacgc gcgtgccgta gaacacgggc gtccccccga gcttctctgg
     1201 gtcgcgctcg attaactgcg tcttattcat gatgcacctc tttcggcgac gccgcgggcc
     1261 ttatcataac agagcgccgt gcgggggcaa gctgtgggta gaaacgcgac ttcagttctt
     1321 tcggccggag tcgcctttcg tcacgggggc gggagccgcc gacgcaccgc gcgcgtagta
     1381 gccctcgcga tagaggagtt ggagcttcgc tttgcggagc gcggggttgg tgatttcgac
     1441 gcggacgcgg cggaagcggc cgtcgcgttg gcggttggcg ggcgtgtagg cgacgaggta
     1501 ctgggcgcgc agctcgtcgt tgatctgggc gaaggcggcg tcgagttcgg cgcgattggt
     1561 ggggaagaag gcgcggccgc ccgtgcgctc ggagaccttg cggaggtcgc cgcgctggag
     1621 cttgccgtgg gggaactctt cgtcgcgaat gccgacggcg tagatgaccg tgttgttctt
     1681 gacggcgaag tcggcggcct cttggcgctc gcggcggctg acggtgtcgt cgccgtcgga
     1741 gagcaggatg acggcgcggc gcgtgcgctc gggcgtcttc gagagcactt cctcgacgct
     1801 cagccacagc gcgtcccaga cggccgtctt acagcgcagt atctgctcct cggggatgtt
     1861 gtcattctcg tcgttacatt cggggctgtc gctggaataa agaactttga ggcggtcgat
     1921 ggcgccgccg agatcggcct tcgcgctcgt cgcgggttgt tcgaaccgga tgatgccggt
     1981 gaaggagagg acggcggccg tgtcgcgctc ggggcgcagc accgagtcga tgaaggcgcg
     2041 ggcggccgat ttttcgtctt ccatgacggc ctcctgcgag gcgctggtat cgacgaggac
     2101 gacgagcgag acgggcagat cggtctcgcg ctggaagagt gtgatctgct gctcgacgcc
     2161 gtcctcgaag agacggatgt cctcgcggcg gagcgtggtg acgaagcgtc gctcgcggtc
     2221 aacggccgtc aaaagtacgc tcgcgaggtc aacgtcaacg cgctcgacct cgtcgtccgg
     2281 ggcttcagtg ggggagggcg tgggcgtggg
//
LOCUS       DASVES010000451        25901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1249427, whole genome shotgun sequence.
ACCESSION   DASVES010000451 DASVES010000000
VERSION     DASVES010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 25901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25901
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249427"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..921)
                     /locus_tag="VF240_18700"
     CDS             complement(61..921)
                     /locus_tag="VF240_18700"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX7177294.1"
                     /translation="MAAQPETVEAPPPPPPLTRPEEGKCLNCGAGLLGEFCHVCGQKK
                     AHAHELGVRHFFGHLLHEITHLDSNKIARTLKALLFQPGLLTEEYLAGRKGRHINPIR
                     VYLTISALFFIVGWATLIDSGGSGRAPNAPPSAPAATQAQPPPPQGAEARYAKTAQKR
                     EKLAALLRFASVLVSGLFLTALFYRSGRFYVEHLIFSLHFYSFDFILRSALALLYMGY
                     NAAGLGLHLPTRVAFYLVLFVYLYLALHRVYRQGRALTLVKAFVLLACEVALFTLIVF
                     GAAFAAMALT"
     gene            complement(1132..3696)
                     /locus_tag="VF240_18705"
     CDS             complement(1132..3696)
                     /locus_tag="VF240_18705"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7177295.1"
                     /translation="MRFSRLTSAALAALLALSTLPAATLADEGMWTFNNLPRAEIKRR
                     YGFEVTDDWLRRAQLATVRFPNGTGSFVSAEGLVLTNYHIVEEAVGALSTAEKDYAKT
                     GFVADAREQELKIPGMELNQLVSIEDVTARVSGAAKPGASAAEAGAAWQAEIGRIVAE
                     STEKTKLKSEVVTLYQGGQYNLYRYRKFTDVRLVFAPEFQTAFFGGDPDNFNFPRFNL
                     DMALVRVYEDDRPHRPESYFKWSKAGAKDGELTFVVGSPGTTQRQNTVAHLEFLRDHG
                     TPYILKSLESQRDALKPYMAKGEEQTRRGQNALNYAENFIKVFKGQLEGLRDKSLMDR
                     KRKEEDALRKFVNSDPRRKKEYADAWDAIAKSRQSLGAYERERRFLGGLTLTDTGAAF
                     NSPLFTYARALVRLADESAKPDAERLPEYTDARRPGLEGALYSTAPIYDDFEQMKLAG
                     SLKLMQDVYGAADPLVRKVLAGKTPEARAAELVSGTKLKDVEFRRQLAAGGKKAIDES
                     TDPLIVLARSVDAEARSVRLRYETEVIGVERGAYSKVARALFAKEGTGLYPDATFTPR
                     LSYGAVKGYEERGKAIPYYTDFAGLYARAAANSFKFPYSLAPRWTEKKSALDLKTPFN
                     FVTTNDITGGNSGSPTFNKELELVGLIFDGNIESLVGAFVYDESVNRAISVDSRGMLE
                     VLRKIYDAKNVAGELTGERSVTVSGQSADAGGFGPAAEEGEVDALVKRLQAETGKDVP
                     VIIGNRDVDPAVVVPASSGSEYKGKGVLNGKALHMPAPAYPPIAKAARAQGTVLVLVV
                     VDQDGKVMAAQAESGHPLLRSAAVAAVRQWQFSPTMLEGKPVRVMGTIGVTFALQQ"
     gene            complement(3868..5445)
                     /locus_tag="VF240_18710"
     CDS             complement(3868..5445)
                     /locus_tag="VF240_18710"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7177296.1"
                     /translation="MESVIRGAKYFCLAAALVVFVCSATPARAQVSPDREQAPKRVGV
                     RLRAFVPNTSASMYFVPTRHGGSVRLTALNLPPAGSLLADASHYVVWAVASEERPLRI
                     GSLMVDAGGNGGLEFARPASFERYSVVVTAETSAEAERPGGVMVFASRAGAVSAFYGE
                     RNEQQLSESQRKALAKEMKKTAGRSGVRDFYSEVDDALDANPGGARVIELLGGELAPK
                     SHGVARVATRNENVYVRTVLRRLPVPSEIGANTYILWGLIPGGRIAYMGSLPSTDFSD
                     ADTYVRVGGIRTDELDLLVSAERRRPVPSPSGQRVLYSNTQSEERGLAYGAIEGMVLD
                     ADSQPVAGATVELRPDSRSVTPDTLPTARTDESGRFFLDGLVPGEHTLYASKETAGYP
                     SAYQAFFIAGDTALPKVAVLDRQVAGGTVVRLGPKAARLRARVLDGRTGQPVEEAEIV
                     LLRDDRQDISMSFGINPADGSFDRLIPSLPLRLKVQAPGYDDWYYGEDGTRENSRVLL
                     LRNDAVEELTIRLRPNK"
     gene            complement(5679..6602)
                     /locus_tag="VF240_18715"
     CDS             complement(5679..6602)
                     /locus_tag="VF240_18715"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX7177297.1"
                     /translation="MRVNTAARRVCVSLLVLAALAVGAAAQEPRNKKMKIYISADMEG
                     IAGVVTGEQLGPAGFEYARFREFMTLEVLTAVETVFEAGATEVLVSDSHGNGQNLLIE
                     RLPKNVTIVRSWPRPLMMMQGIDSSFDAAIFIGYHSGTTNPEGVRAHTISSARLADVR
                     LNGASVPEAGVNAAIAGHFGVPVIMVSGDDAAVKEMQTLLGDVEGAVVKWAYGFHSAR
                     TMTPEAARDLIREKVRRAVGRIRDFKPYKIKAPVTLDVRFKNYRPSEVLAYLSTVERT
                     DAHSVRFVGRDIVEVSKFLEFIMTYEPGLEP"
     gene            complement(6649..8151)
                     /locus_tag="VF240_18720"
     CDS             complement(6649..8151)
                     /locus_tag="VF240_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7177298.1"
                     /translation="MKNRLLLLLLPLLLLLLASAPAAHAQEDIPLFTTDFPAEEFARR
                     RAAVYDAIGREAVAVLQGAPSPVGFTRFRQSNEFYYLCGVEAPHAYLLLDGESRRTIL
                     YLPHRNNSRERSEGKLLSAEDAELVVKLTGVDLVVGTDVLAEHLSRYARGMAGRTLWT
                     PLSPAEGLAVSRDMATRTVGDIASDPWDGRPSREGHFVSLVRTRFPQLEVRDLTPALD
                     RLRLIKSPREIALIEKATRLSGLALIESMRSTEPGVSEYELDAVAKYVYFRNGAQGDA
                     YYSLIASGRNAWFPHYNSGKRVMRDGDFLLMDYAPDVSYYMSDVTRMWPVNGKFSGWQ
                     RELYGFYLECYRAILKAIRPGVTAQAIKQEAAREMAQALGRAKFSKPIYEKAAREFVD
                     GYTRSAQNAQTSLGHWVGMATHDVGDDDGPLRPGMVFTIEPALRVPEERIYIRLEDLI
                     VITETGKRVLPEGLPMDIDAIEKLMREEGMLQRYPRERAPGAAAAGGRTP"
     gene            complement(8293..8547)
                     /locus_tag="VF240_18725"
     CDS             complement(8293..8547)
                     /locus_tag="VF240_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177299.1"
                     /translation="MEHEKRVRIVKRGLRDEAAAPVEARPATATGAAREAKDVVSGWV
                     REHQRRSEEFRNNYSALLGQLGLTAPWAARPPAPAFSAGK"
     gene            8734..9306
                     /locus_tag="VF240_18730"
     CDS             8734..9306
                     /locus_tag="VF240_18730"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7177300.1"
                     /translation="MPTSSQHEVTRLLLRLTEGESTALEDLLPLVYAELRRLAASYLR
                     RERVGHTLQPTALVHEAYIRLIDQTQVRWQNRAHFFGVAAQMMRRILVDYARSQQAEK
                     RGGEFQKLSLDERIDVSGQDRDLDLVALDDALNRLAEIDPQKSKIVEMRFFGGLSVEE
                     TAEVLGVSVPTVKRHWRMAKAWLYGEIKKT"
     gene            9425..16195
                     /locus_tag="VF240_18735"
     CDS             9425..16195
                     /locus_tag="VF240_18735"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177301.1"
                     /translation="MTPERWKRVEEVFQAALDLPAGERQGFVAKACAADGELRSQVEA
                     LVEQYEAAGDFIEEPAVAVTGLRAGGDAVTNPVTGGFVDPALGRRIGAYRIAREIGRG
                     GMGAVYLAERADSEFRRRVAIKLIKRGMDTDFILRRFRNERQILATLDHPYIARLLDG
                     GTTDDGLPYFVMEYIEGLPVYRFCDEQKLTTPERLRLFRHVCDAVHYAHRQLVIHRDI
                     KPSNILVTADGTPKLLDFGIAKLLNPELAAEITIDPTATAMRLMTPEYAAPEQVRGEP
                     VTPATDVYALGVLLYELLTGHRPYRVRHRSPDEMARVVREQEPIVPSERVNSPEDLLP
                     AHPAAGADPDTLSYLYWSRNATAESLRRELSGALDQIVMKALRKAPGERYVSAEEMRD
                     DITRHLDGRTVVAPPVAASSSPARARRPAPPVAGPEPPTGEQSIAVLPLKLLDTGRGG
                     GDTRDDAYLGVGLADALITRLGALRRFALRPTSSVLRFGETDTDPFDAGRQLGVSFVL
                     DGRIRHAGDRIRVNVQLLDVRAGSAVWAGQFDQKFTDVLALEDAISEQVAEALVPQLT
                     GGERLRLQKRGTDSAEAHEAYMRGRYHWNTFTEEGLAHALTHYHEAVARDPNYALAYA
                     GIADYFNWLGVYSVLPFAETSAAAKEAALEAVTLDPALAEGHSALGFARLTHDFEWRP
                     SEADHRRALDLNPNYATGRVWYGYQLMMEGRFEESNAEVRRALEIDPLSAINVHALTW
                     CYYNWRRYDESIPLARRLIALEPRNGIGHLYLGHVLVMSGEHEESISESLRAVEIFGR
                     TPYALSWLAMAYGRVGRPEEARAVLTEVEAMAEQRHVSPYLLALAYCNLGERERALEL
                     LEQACSTRDAWLVWLGVEPQLDPLRSDPRFAELLRRTKNPAAARHASTVAAAAHRRHE
                     DDAAPAQKSIAVLPLRLVSPTSGSGTGDEYLGVGLADALITRLSNIRQIAVRPTSSVL
                     RYQGDDLDPLAAGRELGADYVVAGTVRVAGERIRVSAQLLAVADGQTRWAGNFDENYS
                     DVLQLEDSISGQVADAIIPQLTTEERLRLGKRGTDNAEAYESYMRGRYHWNSFTEEGF
                     AKALVCYNRAIALDPDYALAHAGVADYYNLLGVYAVLPFAETSAAAKESALKAVALDP
                     ALAEGYAALGFSVLCHDFDWEGAERHLRHALELNSNYPTGRLWYGYVLGLSGRHDEAL
                     AHMRRFLQLDTLTPIGYNSRAWVNFYARRFDESVAAARDLSRREPDYGMGQVFLCLVL
                     TQTGNYDEAVAAGQRAVELLGRSPYSLVWLASAYAASGRREEAHALLAEIREMSATRH
                     VSPYLLAMVYCNLGEHERAVVELERACQMRDGRLAWLGVDPQFDPVRADPRVGELLRR
                     THNPLAPPRHLLQSAAAETAYSSGAPRSGERGTGDLAGAKSVAVLPLKVFSTGGAVDT
                     GDEYLGVGLADALVTRLSSVQRIVVRPTSSVLRYRSGDLDPLAAGRELNVDYVVDGNV
                     RRLGDRIRVTAQLLDVREGATRWAGRFDKSAADVLQLEDSISELVAEALVPRLTGDER
                     QQLEKRGTDSPAAYEAYLRGRFHWNIFTEEAFAQALEYYNRAIQIDPAYALAHAAIAD
                     YYNFVGVLCMMPFSEATAAARASALRALELDPTLAEAYSALGLASVCYDFDWTGAETN
                     HQRSVELNPNNATGHQWYSFYLQMAGRFDEAVAEMHRAQEIDPLSSLIMHSLAWCFYH
                     ARRFDESLAAYHNLVKAVPNYPYGLVTYSEALRHVGRHDEAVAAAQRAVELTRDAPFY
                     LTFLAAALAAAGRKEEARDALRRLEEMSRAGRYVSPYMLSFVHVRLGERERALELLEK
                     AYEIVDGWLVWMGVDPELDPLRGDPRFDSLLRRTENPAAARLRRERPVRRAEDTTGGG
                     RDTGATSAQGAPAPPVRPRMTDDDEAQQLYVAGRYYATRRTAEGLRQAIERLERAVER
                     DPQFALAHSELADCYALLNWYVEPPPADAWDQALRYALRAVEADETLAEAHASLGFVK
                     LHYERDWPGAEAELRRAIELRPDNPAARRWYAFTLAAMGRHDEAVAEIEQARELSPRS
                     PVIATAVANVLFLARRFDEAIEQCARALELDPGSVAAHVILRWSYEHRGLAEEALATF
                     EQERVFAGDTPTTRAKRAHVLAATGRKMEAREILRGLLANRAEQRVTAYELAVICSLL
                     NERDQAFEFLAEAEREHAVGYTFVRVDPHLDNLRGDPRFAALLGRTGNLAAPAPHELP
                     APVETDGD"
     gene            complement(16377..16595)
                     /locus_tag="VF240_18740"
     CDS             complement(16377..16595)
                     /locus_tag="VF240_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177302.1"
                     /translation="MLTALVITIALAAGALLLALTSDAFAYELSRGVEAAFDFFEYRA
                     AHKAAERREFGPAADERVAYEASYSTAG"
     gene            complement(16824..17459)
                     /locus_tag="VF240_18745"
     CDS             complement(16824..17459)
                     /locus_tag="VF240_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177303.1"
                     /translation="MTSGVKEKRQTERTIKSRRNRRIRMNFPVTIEVPTEDGRTRVLK
                     ARTVVVSHAGATLDMDEAVPLELGVQVTPPFGGTILAEVNGGWVDRATGRQRVSIRLI
                     DPVSWTSPERLSVPAAGARHEQVSLGVHMRVWQMLAEYTAYLNETSSGALTLGQAAEK
                     IMEELFLSDVNFQDWFAAKIMEDLQAWEKVSVRVSARGRARACAGAGSERG"
     gene            complement(17678..18508)
                     /locus_tag="VF240_18750"
     CDS             complement(17678..18508)
                     /locus_tag="VF240_18750"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7177304.1"
                     /translation="MSDGFNSGGLTRDPAQWHSRGGGAQVAGAQPARSVTRVGVEHAK
                     GTGESPSMKRLLANRLLRALPDEDFARLLPGLEPVSLTTRDSLQEPLGEMRYAYFPED
                     VVISHVTAFADGSAAEVALTGREGVAGLGAVLGGQPASHWARVTVPGSALRMRVELLA
                     GEFCAGAPFRRLLLEQAGRHLAQVAQRSACANRHRLSERLAAWLLLVQERAGSDELTL
                     TQELIANRLGARRAGVTQIACGLQQRGLIRYGRGLIRILDRAGLESAACECYAVLHDN
                     "
     gene            complement(18597..19076)
                     /locus_tag="VF240_18755"
     CDS             complement(18597..19076)
                     /locus_tag="VF240_18755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7177305.1"
                     /translation="MTGTVDTDTHRPNARADAPRRDADAVSGARPCVLVAEDHEDTRF
                     MLRVFLEARGFDVIEAANGEDAIRLSESACPDLILLDGQLPRLDGVGVVSRIRSHDQS
                     RRVPIVFLSGHAEPLARTAAFAAGCDDYLTKPFELVRLASVLRRHLSDGAGPREVSE"
     gene            complement(19421..20101)
                     /locus_tag="VF240_18760"
     CDS             complement(19421..20101)
                     /locus_tag="VF240_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7177306.1"
                     /translation="MVKDASQRAALRLRVMLADDHEVVREGLRALVEAQADMEVVGEA
                     ADGRAAVQRAQELAPDVLVMDISMPGLNGLKATERVKQLCPQVRVLTLTRHTDAGFIQ
                     QLLAAGASGYVLKQSASSELVRAIRTVASGASYLDPQITGKVIGGYVSRQTRFDEEPA
                     KELSEREREVLRLIAWGYSNKEVAARLDISVKTVETHKANTMKKLGLGSRIDIVRYAL
                     LQGWLQDN"
     gene            complement(20214..21620)
                     /locus_tag="VF240_18765"
     CDS             complement(20214..21620)
                     /locus_tag="VF240_18765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7177307.1"
                     /translation="MSEPLKGGGRAEKSVGREDGHDFLIVGVGASAGDSGALEQFLES
                     LNATGSVSHSTVRDADGRIVAGVLTFRAGAEHRMARTAVRESEEQTRLLLNSVEDFAI
                     FTLDLGGHVTRWNKGAEKIFGYTEDEILGRDAGILFTPEDRVRGADKREMKTALEKGR
                     AEDERWHIRKDGSRFYASGVLTTLDAGGELRGYIKVARDLTEREQAEQERADLNRQLE
                     DERGQLEERVRGRTGELEAEVLERRDAEEHVKELLARVVSAQETERRRIARDLHDLLG
                     QQLTALRLNLAAIKDGCGEDSGKLSDQIKQAQAAAEQLDAEVDFLAWELRPAALDAGG
                     LPAALENFIHEWTEHYGVKADFHTTGLGKRRLAPEVEINLYRIAQEALNNVVKHAEAR
                     RVDVILERRDGDVVLIVEDDGKGFDPSAADVCEGSMGLLNMRERAALADGTIEIESAP
                     GEGTTVFARAPARFADGK"
     gene            22007..22729
                     /locus_tag="VF240_18770"
     CDS             22007..22729
                     /locus_tag="VF240_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177308.1"
                     /translation="MAARQSEGDKSAASAAASRQTYEGAARCRRKFLRHFPGGFLDET
                     YLDWERDYKWEVHEQWEGALGRDEYRRLLRAGEFAEAAARAVRVEQRARHSMLFSFEK
                     MALRDAVKTEEGARLFSEGLYEFLHGAGDAERKFARWCEVVGALPRRQTRVLTWPLVT
                     VFGFIAQPSEHIFLKPNVTRIAARAYGFDLRYQSRPNWDTYASMLAFAGVVRRDLRDL
                     RPRDMIDLQSFIWVQGSDEYSD"
     gene            22989..23615
                     /locus_tag="VF240_18775"
     CDS             22989..23615
                     /locus_tag="VF240_18775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7177309.1"
                     /translation="MLQRFFARQLARPSGLFGRLVTARWLDKSSATMNQLTFERLSLQ
                     PDDRVLEVGFGSGYLLKKILSAGPCAHVAGIDPSADMVRLVERRLGKFIRGGKARIRE
                     GSIEALPFGDGEFTKLCTVNTLYFWQEPAVPLSECRRVLREDGLLVLCFNAKEDLERW
                     PGHKYGFRLYELSEIEEELTRSGFGAIDVARGHDPAQGLFYCVNAKAV"
     gene            complement(23637..>25901)
                     /locus_tag="VF240_18780"
     CDS             complement(23637..>25901)
                     /locus_tag="VF240_18780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7177310.1"
                     /translation="IFYTSGTTGKPKGVVIEHGAVAAGVLSLKQYIEFIPGDNVLAVV
                     PAHHVFASLANILMPLATGAGVTYLRALNSVELLKTLQRARVTIFPAVPQVFYLLHKK
                     IFEEVGRKPRPVRLIFRALLRLSFLLRQATGLNMGRKIFSRVHQTFGGHLRLLISAAS
                     YFDPKIIRDFYALGFTVQQGYALTETFGGGAFTPYFKNVIGSAGIPGPGVKVKIVDPD
                     EAGEGELAISGPTLMRGYFNNPEATAQVLRDGWFHTGDLGRMDTGGNYYITGRKKELI
                     VLSSGKKVYPEEVERHYMQSQHIKEMCVLGAADSTGYAQSERLHAVIVPDFDYLKQLK
                     IVNSREAIRSDVELLSVSLPRYKRILSYDIQTEPLPRTSTKKLQRWLVQKQQAPGPAG
                     AAASSAPAAQYNFVEGDERLLEQEASRRVLDVIRQLSRAEGELHPDMNLELDLGFDSL
                     QRTELIVNIEQALGVRLDAAAASQVLTVRELLRAVEGKLGEGPSAGGGHTAAGGVTWK
                     EMLDSANSDEVADEYVHKSPGVLTYVYFLLLKLFLLSSKILFRLKVRGIDNLPPAGPY
                     LICPNHQSYLDGLLVTAVLPYSIFKQLYSLGYTPFFSGGLKDSIARIGKVVPIDANTD
                     LVRAMKVSAIILKANKILLIFPEGGLSCDGQLQSFKKGAPVLAQELGLSVVPTAIKGS
                     FNVWRKGANRIHLAPVEIVFGKPLDFKAQELQAPGGDDGYTLLAQKLKDEVRRLMSTT
                     PGAQVEHEEVADPG"
BASE COUNT         4200 a         8768 c         8556 g         4377 t
ORIGIN      
        1 ttgtccgggc gtcgttcatg gactatcggg gcacgatggc ggccgaggcc gcgccggccg
       61 tcaggtgagc gccatggcgg cgaaggccgc gccgaagacg atgagggtga agagcgccac
      121 ctcgcacgcg agcagtacga acgccttgac gagcgtgagc gcgcgcccct gcctgtagac
      181 ccggtgcagc gcgaggtaga gatagacgaa gagcacgaga tagaaggcga cgcgcgtcgg
      241 caggtgcagg ccgaggccgg cggcgttgta gcccatgtag aggagcgcga gcgccgagcg
      301 caggatgaag tcgaacgagt agaagtggag cgagaagatc aggtgctcga cgtagaagcg
      361 gcctgacctg tagaacagcg cggtgaggaa cagccccgac accaacacgc tcgcgaaccg
      421 cagcagcgcc gccagcttct cgcgcttctg cgcggtcttc gcgtaccgcg cctcagctcc
      481 ctgcggcggc ggcggctgcg cctgcgtggc ggcgggcgca gacggcgggg cattaggtgc
      541 gcggccgctg ccgccgctgt cgatgagcgt cgcccatccg acgatgaaga agagggcgct
      601 gatggtcagg tagacgcgga tggggttgat gtgacggccc ttgcggccgg cgaggtattc
      661 ctcggtcaac agtccgggct ggaagagcag ggccttgagc gtgcgggcga tcttgttcga
      721 gtcgaggtgc gtgatctcgt ggagaaggtg gccgaagaaa tgtcggacgc caagctcgtg
      781 cgcgtgggcc ttcttctgcc cgcagacgtg gcagaactcg ccgagcaatc cggcgccgca
      841 gttgaggcac ttgccctctt cgggccgcgt cagcggcggc ggcggcgggg gcgcttcgac
      901 tgtctcgggc tgtgcggcca agagtctcct tcgcgggcgg tgcgacgtgc ggcggcgaga
      961 tgttcggcgc ggcgcgttga cgagcgccga attttattgc tttacggccc gccgtgtaaa
     1021 tccccttcgc ccgtagaacg aagcggggcc gccggaagtt tccagcagcc ccttcttgtt
     1081 gtcacacctg cgccggacgt gcggccggcc tcgtctactg aagcacgaag ttcactgctg
     1141 aagcgcgaag gttacaccaa tcgtgcccat cacccggacg ggcttgccct ccagcatggt
     1201 tggcgagaac tgccactgcc gcacggccgc caccgccgcc gaccgtagca gcgggtggcc
     1261 gctctcggcc tgcgccgcca tcacctttcc gtcctggtct acaaccacca gcaccagcac
     1321 tgtgccctgt gcccgcgcgg ctttggctat gggcggatat gccggcgcgg gcatgtgaag
     1381 cgccttgccg ttgagcactc ctttcccttt gtactcgcta ccggaactcg cgggaaccac
     1441 gaccgccgga tcgacatccc tgttgccgat gatcaccggc acatcttttc cggtctccgc
     1501 ctgcaaacgc ttcaccagcg catcgacctc accctcctcc gctgcggggc cgaagccgcc
     1561 tgcgtctgct gactgacccg ataccgtcac agaccgctca cccgtcagct cgcccgccac
     1621 gttcttcgcg tcgtagattt tgcgaagcac ttcgagcatc ccgcgcgaat cgacggagat
     1681 ggcgcggttg accgattcgt catagacgaa ggcgccgacg agcgattcga tgttgccgtc
     1741 gaagatgagg ccgacgagtt ccagctcctt gttgaaggtg ggcgaccccg aattgccgcc
     1801 ggtgatgtcg ttcgtcgtca cgaagttgaa gggcgtcttt aagtcgagcg ccgacttctt
     1861 ctccgtccag cgcggtgcga ggctgtatgg aaacttgaag ctgttcgccg cggcgcgcgc
     1921 gtagagaccg gcgaagtcgg tgtaataggg tatggccttg ccgcgctcct cgtagccctt
     1981 gacggccccg taggagagac gcggcgtaaa ggtcgcgtcc gggtagaggc ccgtgccctc
     2041 cttcgcgaag agcgcgcggg cgaccttcga gtaagccccg cgctcgacgc cgatcacctc
     2101 ggtctcgtaa cgcaggcgca cggagcgcgc ttccgcatcg accgagcggg cgagcacgat
     2161 gagcgggtcg gtggactcgt caatcgcttt cttgccgcca gcggcgagct ggcgtcggaa
     2221 ctccacgtcc ttgagcttgg ttcccgagac gagttcggcg gcgcgcgctt cgggcgtctt
     2281 gccggcgagc accttgcgca ccagcgggtc ggccgcgccg taaacgtcct gcatcagctt
     2341 gagcgagccg gcgagcttca tctgctcgaa gtcgtcgtag atgggcgcgg tcgagtagag
     2401 cgcgccttcg aggccgggac ggcgcgcgtc ggtgtactcg ggcaggcgct cggcgtcggg
     2461 cttggcggac tcatcggcga ggcggacgag cgcgcgcgcg taggtgaaga gcggcgagtt
     2521 gaaggccgcg ccggtgtcgg tgagcgtgag gccgccgagg aagcggcgct cgcgctcgta
     2581 tgcgccgaga ctctggcgcg acttcgctat cgcgtcccac gcgtcggcgt actctttctt
     2641 acggcgcggg tcggagttga cgaacttgcg cagcgcgtct tcctctttgc gcttgcgatc
     2701 catcagcgac ttgtcgcgca agccttcgag ctgtcccttg aacaccttga tgaagttctc
     2761 ggcgtagttc agtgcgttct gcccgcgccg cgtctgctcc tcgcctttag ccatgtaggg
     2821 cttgagcgcg tcgcgttgac tctccagcga cttcaggatg tagggcgtgc cgtggtcgcg
     2881 caggaactcc agatgcgcca ccgtgttctg ccgctgggtg gtgccggggc tgccgacgac
     2941 gaaggtcaac tccccatcct tcgcgcccgc cttcgaccac ttgaagtagc tctcggggcg
     3001 gtggggccgg tcgtcctcgt agacgcgcac gagcgccatg tcgaggttga agcgcgggaa
     3061 gttgaagttg tccgggtcgc cgccgaagaa ggccgtctgg aactcgggcg cgaagacgag
     3121 ccgcacgtcg gtgaacttgc gatagcggta gaggttgtac tggccgccct gatagagcgt
     3181 gacgacctcc gacttgagct tcgtcttctc ggtcgattcg gccacgatgc ggccgatctc
     3241 ggcctgccac gccgcgcccg cctcggcggc cgacgcgccc ggcttcgccg cgccgctcac
     3301 gcgcgccgtc acgtcctcga tgctgaccaa ctggttcagc tccatgccgg ggattttcaa
     3361 ttcctgctcg cgcgcgtcgg cgacgaagcc cgtcttggcg tagtccttct cggcagtgga
     3421 gagcgcgccg acggcttcct cgacgatgtg gtagttggtg agcacgagac cctcggcgga
     3481 gacaaaggag ccggtgccgt tggggaagcg gacggtggcg agctgcgcgc ggcgcagcca
     3541 gtcgtccgtg acctcgaagc cgtagcggcg cttgatctcg gcgcggggca ggttgttgaa
     3601 agtccacatc ccttcgtcgg cgagcgtcgc cgccggcagg gtcgaaagtg cgagaagcgc
     3661 cgccagtgcc gcgctcgtga gtctggaaaa tctcatgatc gaatcctcga agaagataga
     3721 tttaagagag agggtttaga aggccgcgag cgcccacgcg ttgtacttga agcttaagga
     3781 tagcgcgcgg ccgggggaaa ctgaaagcgg ccgtgggcgg ccgctttgta aaagttttga
     3841 gtggcgcgcg cgctggccgg gggcggtcta cttgttcggc cgcaggcgga tggtcagctc
     3901 ctcgacggcg tcgtttctaa gcagcagcac gcgtgagttc tcgcgcgtgc cgtcctcgcc
     3961 gtagtaccag tcgtcgtagc ccggcgcctg gactttgaga cgcagcggga gcgaggggat
     4021 gaggcggtcg aaactgccgt cggccgggtt gatgccaaac gacatcgaga tgtcctgccg
     4081 gtcgtcgcgc aggaggacga tctcggcctc ctccacaggc tgccccgtcc ggccgtcaag
     4141 cacgcgcgcg cgcagacgcg ccgccttcgg gccgaggcgc acgacggtgc cgccggccac
     4201 ctgcctgtcg agcaccgcga ctttggggag cgccgtgtcg cccgcgatga agaatgcctg
     4261 ataggccgaa ggatagccgg ccgtctcctt cgaggcgtag agcgtgtgct cgccgggcac
     4321 gaggccgtcg aggaagaagc gcccactctc gtcggtgcgc gcggtgggca gcgtgtccgg
     4381 cgtcacgctg cgcgagtcgg gtcgcagttc gacggtcgcc cccgccacgg gctgactgtc
     4441 ggcgtcgagc accatgccct cgatggcccc gtaggcgagt ccgcgctcct cgctctgcgt
     4501 gttcgagtag agcacgcgct ggcccgaggg cgaagggacg ggacggcggc gctcggcgct
     4561 caccagcagg tccaactcgt cggtgcggat gcccccgacg cggacgtatg tgtcggcgtc
     4621 gctgaagtcg gtcgagggca ggctgcccat gtaggcgatg cgcccgccgg ggatgaggcc
     4681 ccagaggatg taggtgttcg cgccgatctc ggaggggacg gggagacgac gcagcaccgt
     4741 gcggacgtag acgttctcgt tgcgcgtggc gacgcgcgcc acgccgtgcg acttcggcgc
     4801 cagctctccg ccgaggagtt caatcacgcg cgcgccgccg ggattggcgt cgagcgcgtc
     4861 gtcaacctcg gagtagaaat ctctgacgcc ggagcgcccg gccgtcttct tcatctcttt
     4921 ggcgagggcc ttgcgctgcg actcgctgag ctgctgctcg ttgcgctcgc cgtagaaagc
     4981 ggagacggcg ccggcgcgcg aggcgaagac catgacgccg cccggtcgct cggcctcggc
     5041 gctcgtctcg gccgtgacga ccacgctgta acgctcgaac gaagcggggc gcgcgaactc
     5101 cagtccgccg ttgccgcccg cgtcaaccat caggctgccg atgcgtaagg ggcgctcttc
     5161 cgacgccacg gcccacacaa cgtagtggct cgcgtcggcc agaaggctcc ccgccggcgg
     5221 caggtttaga gccgtgagcc gcacggagcc accgtggcgc gtgggcacga agtacatgct
     5281 cgcgctcgtg ttggggacga aggcgcgcag acgcacgccg acgcgcttcg gcgcctgctc
     5341 cctgtctgga gacacctgcg cgcgcgccgg cgtcgccgag cagacgaaca cgacgagggc
     5401 ggcggccaag cagaagtact tcgcgccgcg gattacgctc tccatttgtg ctctccgtcc
     5461 tgaatttaaa atgacaccgg ccggggtgga aacagtcaag gctctcgcgg ggccgacatt
     5521 aagcggaacc ggacgggcgt gtcaacaggt ttctcctgcg cgttgtgcga gtagccgggg
     5581 agaagagttg ggccacggat gagacggatg aagacggatg agcacggatt taatctgtgt
     5641 gaatccgtgc tcatccgtct catccgtgtt cccctcattc acggctccag acccggctcg
     5701 taagtcatga tgaactccag aaacttcgac acctcgacga tgtctcggcc gacgaagcgg
     5761 acgctgtgcg cgtccgtgcg ctcgaccgtc gagagatagg ccagcacctc cgacggccgg
     5821 tagttcttga agcgcacgtc gagcgtcacg ggcgccttta tcttgtaagg cttgaaatcc
     5881 ctgatgcgcc cgacggcccg ccgcaccttc tcgcgaatca ggtcgcgcgc ggcctccggc
     5941 gtcatggtgc gcgccgagtg gaagccgtag gcccacttga cgaccgcgcc ctccacatcg
     6001 ccgaggagcg tctgcatctc tttgaccgcc gcatcgtcgc ccgagaccat gatgacgggg
     6061 acgccgaagt ggcccgcgat ggccgcgttg acgcccgcct cggggacgga cgcgccgttt
     6121 aagcgcacgt cggcgagacg cgcgctggag atggtgtgcg cgcgcacgcc ctcggggttg
     6181 gtggtgccgg agtggtagcc gatgaagatg gcggcgtcga agcttgagtc gatgccctgc
     6241 atcatcatca gcgggcgcgg ccaggagcgg acgatggtga cgttcttcgg caaacgctcg
     6301 atgaggaggt tctgaccgtt gccgtgcgag tcgctgacta agacttcggt cgccccggcc
     6361 tcgaagaccg tctcgacagc cgtgagtact tcgagcgtca tgaactcgcg gaagcgcgcg
     6421 tactcgaagc ccgccgggcc gagttgctcg ccggtgacga cgccggcgat gccctccatg
     6481 tctgccgaga tatagatttt catcttcttg ttccgcggct cctgcgccgc cgcgccgacg
     6541 gcgagggccg ccagcactag cagcgagacg cacacgcggc gcgcggctgt gtttacacgc
     6601 attgacgatc ctcacgccgc ccgcaagagg gcgacgccca acgaactctc aaggggtgcg
     6661 cccgccggcg gccgccgcgc ccggcgcgcg ctcgcgcggg taacgctgga gcatcccctc
     6721 ctcgcgcatc agtttttcga tggcgtcgat gtccatcggc aggccctcgg gcagcacgcg
     6781 cttgcccgtc tcagtgatga cgattaagtc ttcgaggcgg atgtagattc gctcctcggg
     6841 cacgcgcagc gccggctcga tggtgaagac catgccgggg cgcagggggc cgtcgtcgtc
     6901 gcccacgtca tgcgtcgcca tcccgaccca gtggccgagc gaggtctgcg cgttctgggc
     6961 cgagcgcgtg tacccatcga caaattcgcg cgccgccttc tcgtagatcg gcttcgagaa
     7021 cttcgcgcgc ccgagcgcct gcgccatctc gcgcgccgcc tcctgcttga tggcctgcgc
     7081 ggtcacgccg ggacggatgg ccttgagaat ggcgcggtag cattcgaggt agaagccgta
     7141 cagctcgcgc tgccagccgc tgaacttccc gttgacgggc cacatccgcg tcacgtcgct
     7201 catgtagtag ctcacgtcgg gcgcgtagtc catgagcaga aaatctccgt cgcgcatcac
     7261 gcgcttgccg gagttgtagt gcgggaacca cgcgttgcgg ccgctggcga tgagcgagta
     7321 gtaggcgtcg ccctgcgcgc cgttgcggaa gtagacatac ttggcgacgg cgtcaagctc
     7381 atactccgac acgcccggct cggtcgagcg catcgactcg ataagggcca gccccgagag
     7441 gcgcgtggcc ttttcaatca gcgcaatctc gcgcgggctt ttgatgagac gcaggcggtc
     7501 gagcgcgggc gtgaggtcgc gcacttcgag ttggggaaaa cgcgtgcgga cgagcgagac
     7561 gaagtggccc tcgcgcgagg ggcggccgtc ccacgggtcg gaggcgatgt cgccgaccgt
     7621 gcgcgtcgcc atgtcgcggc tcacggcgag tccttcggcc ggactcagcg gcgtccacag
     7681 ggtgcgcccc gccatgccgc gcgcgtagcg cgagaggtgc tcggcgagca cgtccgtgcc
     7741 gacgacgaga tcgacccccg tgagcttcac gacgagttcg gcgtcctcgg ccgagagcaa
     7801 cttgccctcg gagcgctcgc gcgagttgtt gcggtgcggg aggtagagga tggtgcgacg
     7861 cgactcaccg tcgagcagga ggtaggcgtg cggcgcctcg acgccgcaga ggtagtagaa
     7921 ctcgttcgac tgtcggaagc gcgtgaagcc gacgggactc ggcgcgccct gcaagacggc
     7981 cacggcctcc ctgccgatgg cgtcgtagac ggcggcgcgg cggcgcgcga actcttccgc
     8041 cggaaagtcc gtggtgaaga gcgggatgtc ttcctgtgcg tgcgccgcgg gcgcgctcgc
     8101 gagcagcagg agcaggagcg gaagcaacaa gagcagcagt ctgtttttca tgccggcggc
     8161 acctctggcg tgaacgggat ttttatgaca acgtgtccgt gccattgtgg cacagcctgt
     8221 caagccccgt cgtgccccgg cgggcgatgc gatggtggtg gcgccgcgtc gttcggctgg
     8281 cgccggggag tctcacttcc cggcggagaa ggcgggcgcg ggcgggcggg cggcccacgg
     8341 cgccgtgagg ccgagctgtc cgagcagcgc cgagtagttg ttgcggaact cctccgagcg
     8401 ccgctggtgc tcgcgcaccc agcccgagac cacgtctttg gcctcgcgcg cggccccggt
     8461 agcggtcgcg gggcgcgctt cgacgggcgc cgccgcctca tctctcagcc cccgtttgac
     8521 gatgcgtact cgtttttcgt gttccacggt tttccacctc cgacaaacaa cgcgtaggcc
     8581 ggcggcaaaa gggatcaacc acgggggcga tttttttcgg gtgaacgatt ttcgcgaatg
     8641 gcgggcgggc gaaattgata aggcccgacg cgtcgtctat acttgccgcc gtatcctcga
     8701 tacgcgtcgc atttaaatgt cgaaagttcc cccgtgccca cgtcgtcgca gcatgaagta
     8761 acacggctgc tcttgcgcct caccgagggc gagagcacgg ccctcgaaga cctgctgccg
     8821 ctcgtctacg ccgagctgcg gcggctcgcc gccagctatc tgcggcgcga gcgcgtcggc
     8881 cacactctcc agcccaccgc cctcgtccac gaggcgtaca tccggctcat cgaccagacg
     8941 caggtgcgct ggcagaaccg cgcgcacttc ttcggcgtgg ccgcgcagat gatgcgccgc
     9001 atcctcgtcg attacgcgcg ctctcagcag gccgagaagc gcggcggcga gtttcagaag
     9061 ctctcgctcg acgagcgcat cgacgtgtcg ggacaagacc gtgacctcga cttagtcgca
     9121 ctcgacgacg ccctcaaccg cctcgccgag atcgacccgc agaagtcgaa gatcgtggag
     9181 atgcgcttct tcggcggcct ctccgtcgag gagacggccg aggtcctggg cgtcagcgtc
     9241 cccaccgtca agcgccactg gcgcatggcg aaggcatggc tctacggaga aataaagaaa
     9301 acgtgataag tgatgagtga taagtgataa gaaagaaaag aagctgttag ctgttggctg
     9361 ttagctaaca gctataagct aatagctctt atcacttatc actcatcact tatcactcct
     9421 agcaatgacc cccgaacgct ggaagagagt cgaagaggtt tttcaggcgg cgctggattt
     9481 gccggccggc gagcggcagg ggtttgtcgc gaaggcgtgc gccgcagacg gcgagctgcg
     9541 ctcacaggtc gaggcgctgg tcgagcagta cgaagcggcg ggcgacttca tcgaggagcc
     9601 ggccgtggcg gtgacgggcc tgcgcgcggg cggcgacgcc gtgacgaacc ccgtcacggg
     9661 cggcttcgtc gatccggctc tcggccggcg catcggcgct taccgcatcg cccgcgagat
     9721 cggccgcggc ggcatggggg ccgtctatct ggccgagcgc gccgactcgg agtttcgccg
     9781 ccgcgtcgcc atcaagctca tcaagcgcgg gatggacacc gacttcatcc tccgccgctt
     9841 ccgcaacgaa cgccagattc tcgccacgct cgaccatccc tacatcgccc gcctgctcga
     9901 cggcggcacc acggacgacg gcctgcccta cttcgtcatg gagtacatcg agggcctgcc
     9961 cgtctaccgc ttctgcgacg agcagaagct gacgacgccc gagcgtctcc gtctcttccg
    10021 acacgtctgc gacgccgtcc actacgcgca ccggcagctc gtcatccacc gcgacatcaa
    10081 gccctcgaac atcctcgtca cggccgacgg caccccgaag cttttagact tcggcatcgc
    10141 caaactcctc aaccccgaac tcgccgccga gatcaccatc gacccgacgg cgacagccat
    10201 gcgcctgatg acgcccgagt acgccgcgcc cgagcaggtg cgcggcgagc cggtcacgcc
    10261 cgccacggac gtgtatgcct tgggcgtctt gctctacgag cttttgacgg ggcaccggcc
    10321 ctaccgcgtg cgccaccgct cgccggacga gatggcccgc gtcgtgcgcg agcaggagcc
    10381 aatcgtgccg agcgagcgcg tcaacagccc cgaagacctt ctgcccgcac atccggccgc
    10441 cggcgccgac cccgacacgc tcagctatct ctactggagc cgcaacgcca cggccgagag
    10501 cctgcgccgc gaactctccg gcgcgctcga ccagatcgtc atgaaggctc tgcgcaaagc
    10561 ccccggtgaa cgctacgtct cggccgaaga gatgcgcgac gacatcacgc gccacctcga
    10621 cggacgaacg gtcgtggccc cgcccgtcgc cgcgtcgtcg tcgcccgcgc gcgcgcgccg
    10681 ccccgcgccg ccggtcgccg gccccgagcc gccgaccggc gagcaatcaa tcgccgtcct
    10741 cccgctcaag ctgctcgaca cgggccgcgg cggcggcgac acgcgcgacg acgcctacct
    10801 cggagtcgga ctcgccgacg cgctcatcac gcgcctcggc gcgttgcgac gtttcgcttt
    10861 gcggccgaca agctccgtgc tgcgcttcgg cgagaccgac accgacccct tcgacgcggg
    10921 ccggcagctc ggcgtcagct tcgtcctcga cggccgcatc cgccacgccg gcgaccgcat
    10981 ccgcgtcaac gtccaactgc tcgacgtgcg ggccggctcc gccgtctggg ccgggcagtt
    11041 cgaccagaag ttcacggacg tgctcgcgct cgaagacgcc atctcggagc aggtggccga
    11101 ggcgctcgtc ccgcagctca cgggcggcga acggctccgt ctgcaaaagc gcggcaccga
    11161 cagcgccgag gcgcacgaag cctacatgcg cgggcgctac cactggaaca ccttcaccga
    11221 agagggcctg gcccatgccc tcacccacta ccacgaggcc gtcgcccgtg acccgaacta
    11281 cgcgctcgcc tacgccggca tcgccgacta tttcaactgg ctcggcgtct actcggtgct
    11341 tcccttcgcc gaaacctcag ccgccgcgaa ggaggccgcg ctcgaagccg tcaccctcga
    11401 ccccgcactc gccgaggggc actcggcgct cggcttcgca cgcctcacgc acgacttcga
    11461 gtggcggcct tcggaagcgg atcaccggcg cgcgctggat ttgaacccga actatgcgac
    11521 ggggcgtgtc tggtacggct accagctcat gatggagggc cgtttcgagg agtccaacgc
    11581 cgaggtgcgc cgcgcgctgg agatcgaccc gctctcggcc atcaacgtcc acgcgctcac
    11641 ctggtgctac tacaactggc ggcgctacga cgaaagcatc cccctcgccc gccgcctcat
    11701 cgcccttgag ccgcgcaacg gcatcggcca cctctacctc ggccacgtcc tcgtcatgtc
    11761 gggcgagcac gaggagtcga tctcggagtc tctgcgggcc gtcgagatat tcggtcgcac
    11821 gccttacgcg ctctcgtggc tggcgatggc ctacggccgc gtggggcgtc ccgaagaagc
    11881 acgcgccgta ctcacagagg tcgaggcgat ggccgaacag cgccacgtct cgccctacct
    11941 gctcgcactc gcctactgca atctggggga gcgcgagcgc gccctcgaac tcttagagca
    12001 ggcgtgctcg acccgcgatg cgtggctcgt ctggctcggc gtcgagccgc agctcgaccc
    12061 cctgcgctct gacccgcgct tcgccgagct gttgcggcgg acgaagaatc cggccgccgc
    12121 ccgccacgcc tcgaccgtcg ccgccgccgc gcatcgccgg cacgaagacg acgccgcgcc
    12181 ggcgcagaag tcaatcgccg tcctcccctt gcgtctcgtc agcccgactt cggggagcgg
    12241 cacgggcgac gagtatctcg gcgtgggtct ggccgacgcg cttatcacgc gcctgtcgaa
    12301 catccggcag atcgccgtgc gccccacgtc gagcgtcctg cgctatcagg gcgacgacct
    12361 cgacccgctc gcggccggcc gcgagttggg ggccgactac gtcgtcgccg gcaccgtccg
    12421 cgtcgccggc gagcgcatcc gcgtcagcgc gcaactgctc gccgtcgccg acggccagac
    12481 gcgctgggcc ggtaacttcg acgagaacta ttcggacgtg ctgcaactcg aagactcgat
    12541 ctccgggcaa gtggccgacg ccatcatccc gcagttgacc acggaggagc gcctccgtct
    12601 cggcaagcgc ggcaccgata acgccgaggc ttacgagtcg tacatgcgcg ggcgctacca
    12661 ctggaactct tttacggagg agggcttcgc caaggcgctc gtctgctaca accgcgccat
    12721 cgccctcgac cccgactacg cgctcgcgca cgccggcgtc gccgactatt acaatctgct
    12781 cggcgtctat gccgtgctgc ccttcgccga aacctcggcc gccgcgaaag agtcggcgct
    12841 caaggccgtc gccttagacc ccgcgctcgc cgaaggctac gccgcgctcg gcttcagtgt
    12901 gctctgccac gacttcgact gggagggggc cgagcgacac ctgcgccacg ccctcgaact
    12961 caactccaac tacccgacgg gccgcctctg gtacggctac gtgctcggac tttcgggccg
    13021 ccacgacgag gcgctggcgc acatgcgccg cttcctccag ctcgacacgc tcacacccat
    13081 cggctacaac tcccgcgcct gggtcaactt ctacgcgcgc cgcttcgacg agagcgtcgc
    13141 ggccgcgcgc gacctctcgc ggcgcgagcc ggactacggc atggggcagg tcttcctgtg
    13201 cctcgtgctc acacagaccg gcaactacga cgaggccgtc gcggcgggcc aaagagccgt
    13261 cgagctgctc ggccgcagcc cctactcgct cgtctggctc gcctcggcct atgctgcctc
    13321 ggggcggcgc gaggaggcgc acgcgctgct cgccgaaatc cgcgagatgt cggccacgcg
    13381 ccacgtctcg ccctacctgc tcgcgatggt ctactgcaat ctgggcgaac acgagcgcgc
    13441 tgttgtggag cttgagcgcg cgtgccagat gcgcgacggc cgtctcgctt ggctcggcgt
    13501 cgatccgcag ttcgaccccg tgcgcgccga cccacgcgtc ggcgaactcc tgcgccgcac
    13561 gcacaacccg ctcgcgccgc cgcgacacct cctccagtcg gccgccgccg agaccgccta
    13621 cagctccggg gcgccgcgct cgggcgagcg cggcacgggc gacctcgcgg gcgcgaagtc
    13681 cgtcgccgtc ctcccgctca aggtgttcag caccgggggc gccgttgata cgggcgacga
    13741 atatctcggc gtcggactcg ccgacgcgct cgtcacgcgc ctctccagcg tccaacgtat
    13801 cgtcgtgcgc cccacgtcga gcgtcctgcg ctaccgcagc ggcgacctcg acccgctcgc
    13861 ggccgggcgc gagttgaacg tcgattacgt cgtggacgga aacgtccgcc gactcggcga
    13921 ccgcatccgc gtcaccgcgc aactgctcga cgtgcgcgag ggcgcaacgc gctgggccgg
    13981 tcgcttcgac aagagcgcgg ccgacgtgct ccagctcgaa gactcgatct ccgaactcgt
    14041 ggccgaggcg ctcgtcccgc gcctcaccgg cgacgagcgc cagcagctcg aaaagcgcgg
    14101 caccgacagc cccgccgcct acgaagccta cctgcgcggg cgcttccact ggaacatctt
    14161 caccgaggag gccttcgcgc aggcgctcga atactacaac cgcgccatcc agatcgaccc
    14221 cgcctacgcg ctcgcccacg cggccatcgc cgactactac aacttcgtcg gcgtcctctg
    14281 catgatgccc ttctccgagg ccacggccgc cgcgcgtgcg tcggcgctac gcgcgctgga
    14341 gttagacccc acgctcgccg aagcctactc cgccctcggc ctcgcctccg tctgctacga
    14401 cttcgactgg acgggcgccg agaccaacca ccagcgcagc gtcgagctaa atccgaacaa
    14461 cgcgaccggc caccagtggt acagcttcta cctccagatg gccgggcgct tcgacgaggc
    14521 cgtcgccgag atgcaccgcg cgcaggagat cgacccgctc tcctcgctca tcatgcactc
    14581 actcgcctgg tgcttctacc acgcgcgccg cttcgacgaa tcgctggccg cctaccacaa
    14641 tctcgtcaag gccgtgccca actaccctta cggcctcgtc acctacagcg aggcgctccg
    14701 ccacgtcggc cggcacgacg aggccgtcgc cgccgcccaa agagcagtcg agctgacgcg
    14761 cgacgcgccg ttctacctca ccttcctcgc cgccgccctc gccgccgccg ggcgcaagga
    14821 ggaggcgcgc gacgcgctcc gccggctcga agaaatgtcg cgcgccgggc gctacgtctc
    14881 gccctacatg ctctcgttcg tccacgtccg tttgggcgag cgcgagcgag cgcttgagct
    14941 tttggagaag gcttacgaga ttgttgacgg ctggctcgtc tggatgggcg ttgacccgga
    15001 actcgacccg ctgcgcggcg acccacgctt cgactctctg ctgcggcgca cggagaaccc
    15061 ggccgcggcg cgtctcaggc gggagcggcc cgtgcgccgc gccgaagaca cgacgggcgg
    15121 cgggcgcgac accggcgcca cctccgcaca gggcgcgccc gcgccccccg tccggccgcg
    15181 aatgacggac gacgacgagg cccaacagct ctacgtggcc ggtcgctact acgcgacgcg
    15241 gcgcacggcc gaaggactcc gacaggccat tgaaagactg gagcgcgccg tcgagcgcga
    15301 cccgcaattc gcgctcgccc actcggagct ggccgactgc tacgcgctgc tcaactggta
    15361 cgtcgaaccg ccgcctgccg acgcgtggga tcaggcgctt cgttatgcac ttcgcgccgt
    15421 cgaagcggat gagactctgg ccgaggcgca cgcctcgctc ggcttcgtca agctccacta
    15481 cgagcgcgac tggccggggg ccgaagccga gcttcggcgc gccatcgagc tgcggcccga
    15541 caacccggcc gcgcgccgct ggtacgcctt cacgctcgcc gccatgggcc gccacgacga
    15601 ggccgtcgcc gagatcgagc aggcgcgtga gctttcgccc cgctcacccg tcatcgccac
    15661 ggccgtcgcc aacgtcctct tcctcgcgcg ccgcttcgac gaggccatcg agcagtgcgc
    15721 tcgtgcgctc gaactcgatc ccggctccgt ggccgcccac gtcatcctgc gctggtctta
    15781 cgagcaccgc ggcctcgccg aagaggcgct tgccaccttc gagcaggaac gcgtcttcgc
    15841 cggcgacacg ccgaccacgc gcgccaagcg cgcgcacgtc ctcgccgcca cgggccgaaa
    15901 gatggaggcc cgcgaaatcc tgcgtggcct gctcgccaac cgcgccgagc agcgcgtcac
    15961 ggcctacgag ctggccgtca tttgttctct gcttaatgaa cgcgatcagg cttttgaatt
    16021 cttagccgag gccgagcgcg agcacgccgt cggctacacc ttcgtccgcg tcgatccgca
    16081 cctcgacaac ctgcgcggcg acccacgctt cgccgcgctg ctcggacgca ccggcaacct
    16141 cgcggccccc gccccccacg agttgcccgc acccgtcgag acggacggcg attgatccgc
    16201 ccttcccccc tttaagctcg ccaccccgca cacacacagt ggtatggcat agcattcacc
    16261 aaggaatgtt tagaggcggt ctgtgagcgc cggctcaacc catgcggtga cttgagccgg
    16321 cggtcacaga ccgcctctaa acggacgttt gactgaagtg ctaaccgctc gaagcgtcag
    16381 cccgccgtcg agtaggacgc ctcataggcg acacgctcgt cagccgccgg cccgaactcc
    16441 cggcgctccg ccgccttgtg cgccgccctg tactcgaaga agtcgaaggc ggcttcgacg
    16501 ccgcgcgaca gctcgtaagc gaatgcgtcg gacgtgagcg cgagcagcag tgcgcccgcc
    16561 gcgagtgcga ttgtgattac cagtgccgtc aacatctctc atctctcccc caatatttcc
    16621 cgccgcccgg ctcgcccgtt cggcctcaac tattaacgcg caaaccgcgg cccgaaagga
    16681 tcacgcgcct cgcgctttca accacggccc gccgtctttc gtcgcggccc gtgtcacgca
    16741 atgaacgacg agcggcaaac gtacttgcac gctaccatca cgtacttgct cctagcgccg
    16801 aagcggcaaa agggttcgtc cgttcagccg cgctcgctgc cggctccggc gcaggctcgc
    16861 gcccttccgc gcgcgctcac acgcacgctc accttctccc aggcttgcag gtcttccatg
    16921 atcttcgccg cgaaccagtc ctgaaaattc acatccgaga ggaacaactc ctccataatc
    16981 ttctcggcgg cctgcccgag cgtgagggcg ccactggaag tttcgttcag gtaagcggtg
    17041 tactcggcga gcatctgcca cacacgcatg tggacgccga gactcacctg ctcatggcgt
    17101 gcgccggcgg cgggcaccga gagcctttcg ggcgaagtcc aggagacggg gtcgatgagg
    17161 cgaatgctga cgcgctggcg gcccgtcgcg cggtctaccc agccgccgtt gacctcggcc
    17221 agaatcgtgc cgccgaaggg cggcgtaacc tgcacgccca gctccagcgg gacggcctcg
    17281 tccatgtcga gcgtcgcgcc ggcgtgactc accacgacgg tgcgggcctt gagcacgcgc
    17341 gtgcgtccgt cctcggtcgg cacctcgatg gtgacgggga agttcatcct gatgcgccgg
    17401 ttccggcgcg acttgatggt gcgttcagtt tgacgcttct ctttcactcc gcttgtcata
    17461 aagacctcat ttgccgcacg gatgtggggc cgcggcccct cgccgggaga cgttggcaaa
    17521 tgaagtgcca gccgatgccg aagacgcttg cacagtgttt ggtgacggcg cgcggccgat
    17581 ttgcgtctgt cgggtgtgac aaaaaaagtg agaggccgaa gacacttctt cggcctctca
    17641 ctcattcgac gcggacgcgc gcgtcgattg tcttgttcta gttgtcgtgc aggacggcgt
    17701 agcactcgca ggcggctgac tccagacccg ctcggtcgag gatacggatg aggccgcggc
    17761 catagcggat gaggccgcgc tgctggaggc cacaggcgat ctgcgtgacg ccggcgcggc
    17821 gggcgccgag ccggttggcg ataagttcct gcgtgagggt caactcgtcg gagccggcgc
    17881 gctcctgcac gagcagaagc cacgcggcga gacgctcgga caggcggtgg cggttggcgc
    17941 aggccgagcg ttgcgcgact tgcgccaaat gccggcccgc ctgttcgagc agcaggcggc
    18001 ggaagggcgc gccggcgcag aactcgccgg cgaggagttc gacgcgcatc cgcagggcgc
    18061 tgcccggaac ggtgacgcgc gcccagtgcg acgcgggctg gccgccgagg acggcgccga
    18121 ggccggcgac gccctccctg ccggtcaggg cgacctcggc ggcgctgccg tcggcgaagg
    18181 cggtgacgtg cgagatgacc acgtcctcgg gaaagtaggc gtagcgcatt tcgccgagcg
    18241 gttcctggag gctgtcgcgc gtggtgagcg agacgggttc gaggccgggc aatagacgcg
    18301 cgaagtcttc gtcgggcagc gctctcaaga gtctgttggc gagcagacgc ttcatcgagg
    18361 gcgactcgcc cgtccccttc gcgtgctcga ctcccacccg tgttactgac cgcgcaggct
    18421 gcgccccggc gacctgcgcg ccgccgccgc gcgaatgcca ctgcgcgggg tcgcgtgtga
    18481 ggccgccgct gttgaaacca tcggacatcg tcgtgttacc gccttccctt atctcaaacg
    18541 tcgtttcgtc gcccctcgcc gtcgggcgct ggttccgcct tcggacgtgt cggtgctcat
    18601 tcggacacct cgcgggggcc ggctccgtcg ctcaagtgtc gcctcagcac ggaagcgagg
    18661 cggactaact cgaagggctt cgtcaggtag tcgtcgcacc cggcggcgaa ggcggccgtg
    18721 cgcgccagcg gctcggcgtg gcccgagagg aagacgatgg ggacgcgccg ggactggtcg
    18781 tgcgagcgga tgcgagacac gacgccgacg ccgtcgaggc gcggcaactg gccgtcgagc
    18841 aggatgaggt cggggcacgc gctctcgctg aggcggatgg cgtcctcgcc gttggcggcc
    18901 tcgatcacgt cgaagccgcg cgcctccaga aatacacgga gcatgaagcg cgtgtcctcg
    18961 tggtcttcgg cgacgagcac acacggacgc gccccggaca cggcgtcggc gtcgcgccgc
    19021 ggcgcgtccg cgcgggcgtt gggccgatgc gtgtccgtgt ccacagttcc ggtcatgcga
    19081 ggtagtcctc gaagagtcgc tgctggattc atcgctgatt gctgatcacg gccggggcgg
    19141 cgaaagtgtt tgaaaatttc gagcaggtta gcgtgcccgc tgccccccgg cgcgttctgt
    19201 ccggcgagtc gtggtgcgaa agtctcgcgc ccgtcgtggg cgagcgacgc ccgacctgtg
    19261 ccgcctattt gtgcgaatgg agagtatgac gtggcgggag cgatgcgtct atcaggagaa
    19321 acgaggccga ctagggtttt tccctaacca gcggagcagg gattaaacgg ctgtaaatat
    19381 gccggttaac ggaggcaggc ggagacggga gaggcgcgct tcagttatct tgcagccacc
    19441 cctggagcag ggcgtagcga acgatgtcga tgcggctccc gaggccgagc ttcttcatcg
    19501 tgttcgcctt gtgggtctcg accgtcttga cgctgatgtc gagacgggcg gcgacctcct
    19561 tgttgctgta gccccacgcg atgagacgca ggacttcgcg ttcgcgctcg ctcaactcct
    19621 tcgccggctc ttcgtcgaaa cgggtctggc ggctgacgta gccgccgatg actttgccgg
    19681 tgatctgcgg gtcgaggtag ctggccccgg aggcgacggt gcggatggcg cgcacgagtt
    19741 ctgacgaggc gctttgcttg aggacgtagc cggaggcgcc ggcggctaag agttgttgga
    19801 tgaaaccggc gtcggtgtgg cgcgtgagcg tgagcacgcg cacctgcggg cagagttgct
    19861 tcacccgctc ggtcgccttc aggccgttga gtccgggcat cgagatgtcc atcacgagca
    19921 cgtcgggcgc gagttcctgc gcgcgctgga cggccgcgcg gccgtcggcc gcctcgccga
    19981 ccacttccat gtcggcctgc gcctcgacga gcgccctcag cccctcgcgc accacctcgt
    20041 ggtcgtcggc gagcatgacg cggagcctca gcgctgcccg ttgcgatgcg tctttgacca
    20101 tgcgggcctt cacttcttta agttcaaagt tcaaagttca aagttcaaag aacaaaagcg
    20161 cgccggcggc tgttgacttt gaactttgaa ctttgaactt tgaactcatt atttcacttc
    20221 ccgtcggcga agcgcgcggg ggcgcgcgcg aagacggtcg ttccctcgcc cggcgccgac
    20281 tcgatctcta tcgtgccgtc ggcgagtgcg gcgcgctcgc gcatgttgag cagacccatg
    20341 ctgccctcgc acacgtcggc cgcgctcggg tcgaagccct tgccgtcgtc ttcgacgatg
    20401 agcacgacgt cgccgtcgcg ccgttccaga atcacgtcca cgcggcgcgc ctcggcgtgc
    20461 ttgacgacgt tgttgagagc ctcctgcgcg atgcggtaga ggttgatctc gacctcgggc
    20521 gcgaggcggc gcttgcccag acccgtggtg tggaagtcgg ccttgacgcc gtagtgctcc
    20581 gtccactcgt ggatgaagtt ttcgagcgcg gccggcagtc cccccgcgtc gagcgcggcc
    20641 ggccgcagct cccacgccag aaaatccacc tcggcgtcga gctgctcggc cgcggcctgc
    20701 gcctgtttga tctggtcgga gagcttgccg ctgtcctcgc cgcagccgtc cttgatggcc
    20761 gcgaggttca ggcgcagcgc cgtcaactgc tgacccaaca aatcgtggag gtcgcgcgcg
    20821 atgcggcggc gctcggtctc ctgcgcgctg acgacgcgcg cgagcagctc cttgacgtgc
    20881 tcctcggcgt cgcgacgctc cagcacctcc gcctcaagct cgccggtgcg tccccggacg
    20941 cgctcctcca gctgcccgcg ctcatcttcg agctgcctgt tcaggtcggc gcgctcctgc
    21001 tcggcctgct cacgctcggt gaggtcgcgc gcgactttga tatagccgcg cagctcgccg
    21061 ccggcgtcga gggttgtcag gacgccggag gcgtagaagc gcgagccgtc cttgcggatg
    21121 tgccaacgct cgtcctcggc gcggcccttt tcgagcgccg tcttcatctc ccgcttgtcg
    21181 gcgccgcgca cgcggtcttc cggcgtgaag aggattccgg cgtcccggcc cagaatttcg
    21241 tcctcggtgt agccgaagat tttttccgcg cccttgttcc agcgggtgac gtggccgccg
    21301 aggtcgaggg tgaagatggc gaagtcttcg acggaattga ggagcagccg cgtttgctcc
    21361 tccgactccc ggacggccgt ccgcgccatc cgatgctcgg cgccggcgcg gaaggtcaag
    21421 acgccggcga cgatgcggcc gtcggcgtcg cggacggtcg agtgactgac gctgcccgtg
    21481 gcgttcagcg actcgaggaa ctgctccagc gcgcccgagt cgccggccga cgcgccgacg
    21541 cccacgatca gaaagtcgtg accgtcctca cgccccacac tcttctcggc gcgcccgccg
    21601 cccttaaggg gttcgctcat aaaatcttct tcttctcatc tgttggaacg gccgcgcgcg
    21661 gttgggccgg gaaggcccgc cgctgttatt tcaacccgct ggaggagatt tacaaacagg
    21721 gtacggcggc tgacgcgcgg agtataaaac acatagtgcg cctcgtacag caaacgatct
    21781 gtgtggtagc acacacccgt ccgccgtcac cccggcgcgc gacgcataca ccagcgcgcg
    21841 cgcgtgtggt ataacgctcg gcatccccct gattaatccc ccgcggacgc gggcgcccgg
    21901 aagtcgggcg agacggtcgc ccccgacgcc gggcgaccgg agagcgccct ttggcgtccg
    21961 cccttgaagt tttcgccgcg tccgacgcgc agaaagggtt gatgagatgg ccgcacgtca
    22021 gagtgaaggt gacaaaagcg cggcgagcgc cgcggcttcc cgccagacat atgagggcgc
    22081 cgcgcgctgc cgccgcaagt tcctgcgaca cttccccggc ggcttcctcg acgagaccta
    22141 cctcgactgg gagcgcgact acaagtggga ggttcacgag cagtgggagg gggcgctcgg
    22201 ccgcgacgag tatcgccggc tgctgcgcgc gggtgagttc gccgaagcgg ccgcgcgcgc
    22261 cgtgcgcgtc gagcagcgcg cccgccacag catgctcttc tcgttcgaga agatggcgct
    22321 gcgcgacgcc gtcaagacgg aggagggcgc gcgcctcttc agtgaaggac tctacgaatt
    22381 tctccacggc gcgggcgacg ccgagcgcaa gttcgcgcgc tggtgcgagg tggtgggcgc
    22441 gctgccgcgc cggcagacgc gcgtgctgac gtggccgctc gtcacggtct tcggcttcat
    22501 cgcgcaaccc agcgagcata tctttctcaa gccgaacgtc acgcgcatcg cggcccgcgc
    22561 ctacggcttc gacctccgct accagtcgcg gcccaactgg gacacctacg cgagcatgct
    22621 cgccttcgcc ggcgtcgtgc ggcgcgatct gcgcgacctg cggccccgcg acatgatcga
    22681 cctccagtcg ttcatctggg tgcaaggctc cgacgagtac tcggactgaa gacgaacgcg
    22741 gtaaagcgcg tcgcaaaact gtaggggctg gggaagtccg cagcccgcat cgtgggaacc
    22801 aattaacatg gatggacagg atgaacagga tgaagaaaag atgacttcag atttgagatt
    22861 tcaaattctt tatcctgccc gtcctgtcca tccatgttac ttcctccgtt ggcagcgggc
    22921 cgcggacaag ccctgcccct acagtcgcga cgcatgacga gtggttctgc aacggacttt
    22981 tcatcacggt gcttcaaaga ttttttgcca gacaactcgc ccggccctcc ggcctcttcg
    23041 gaagactcgt cacggcgcgc tggctcgaca agtccagcgc gacgatgaac cagttgacct
    23101 tcgagcggct ttccctccaa ccggatgaca gggttcttga agtcggcttc ggcagcggct
    23161 acctgctgaa gaaaattctc tccgcggggc cgtgcgcgca cgtggcgggc attgacccgt
    23221 ccgccgacat ggttcgattg gtggagcggc ggctgggtaa atttatcaga ggcgggaagg
    23281 cgcgtatccg cgaaggctca atcgaagctc tgccgttcgg ggacggcgag tttacgaagc
    23341 tctgtacggt caacacgctc tacttctggc aagagccggc cgtccccctg tccgagtgtc
    23401 gtcgggtgct gcgcgaggac ggcctgctcg tgctctgctt caacgcgaag gaagacctgg
    23461 agcgttggcc gggccacaag tacggcttca ggttgtatga gctgagcgag atcgaagaag
    23521 aactgacgcg ctccggcttc ggcgcgatag acgtggcgcg tggacacgac ccggcgcagg
    23581 ggttgttcta ctgtgtgaac gcgaaagccg tctgagcgcg cgtcgcggcc ttcgcgtcag
    23641 ccggggtcag ctacttcctc atgctccacc tgcgcgcccg gcgttgtaga catcaaacgc
    23701 cttacttcat ccttcagctt ttgtgcaaga agagtgtagc cgtcatcacc gccgggcgcc
    23761 tgtagctcct gcgctttgaa gtccaacggc ttgccgaaaa cgatctcgac gggcgcgagg
    23821 tgaatccggt tagcgccttt tcgccaaaca ttgaacgaac ctttaatcgc cgtcggcacc
    23881 acggacaggc ccaactcctg cgccaacacg ggcgctccct tcttgaaact ctgaagctgg
    23941 ccgtcacagc tcaagccccc ctcggggaag atgagcagga ttttatttgc cttgagaatg
    24001 atggcgctga ccttcatcgc gcgcacgagg tcggtgttgg cgtcgatggg cacgacctta
    24061 ccgatgcgcg cgattgaatc cttgaggccg ccgctgaaga aaggcgtgta gccgagcgag
    24121 tagagttgtt tgaagatgga gtaagggaga accgcggtga ctaaaaggcc gtcgaggtag
    24181 ctctgatggt tgggacagat gaggtacggc cccgcggggg ggagattgtc gatgcctctg
    24241 actttgagcc tgaacaggat ttttgatgac aggagaaata atttgagcag aaggaaatag
    24301 acgtaggtca gcacgcccgg agacttgtgg acatactcgt cggccacctc atcgctgttc
    24361 gccgaatcca gcatctcttt ccaggtgacg ccgcccgccg ccgtgtgccc gccgccggcg
    24421 ctcgggcctt cgcccagttt gccctccacc gccctcagga gttcgcggac ggttaagacc
    24481 tgactggcgg ccgcggcgtc gagccggaca cccagggcct gctcgatgtt cacaatcagc
    24541 tcggtgcgct ggagggagtc gaaaccgagg tcaagctcaa ggttcatgtc cgggtgcaac
    24601 tcgccttcgg cgcgagacag ttggcgaatc acgtcgagca ctcgccgcga cgcctcctgt
    24661 tcgagcaggc gttcgtcgcc ctcgacaaaa ttgtattgcg ccgccggcgc ggaactggcc
    24721 gccgcgcccg cggggccggg agcctgctgc ttctgcacca accatctctg gagcttcttg
    24781 gtcgaagtgc gcggcagagg ctccgtttga atgtcgtagg agagaattct tttgtaccgg
    24841 ggcagagaca ccgacagaag ctccacgtcc gagcggatgg cctctcggga attgacgatc
    24901 ttgagctgct tcagataatc gaagtccggc acgatgacgg cgtgcagccg ctcggattgc
    24961 gcgtagccgg tggagtcggc cgcgccgagc acgcacatct ccttgatgtg ctggctctgc
    25021 atgtagtggc gctccacctc ttcggggtag accttcttcc cggagctgag gacgatcaac
    25081 tccttcttgc ggccggtgat gtaatagttg ccgccagtgt ccatgcggcc gagatcgccg
    25141 gtgtgaaacc acccgtcgcg gagcacctgc gccgtcgcct cggggttgtt gaaatagccg
    25201 cgcatcaggg tcggcccgga gatggcgagt tccccttctc ccgcttcgtc cgggtcaacg
    25261 attttgacct tcacgccggg gccgggaatg ccggccgagc cgatgacatt tttaaagtac
    25321 ggggtgaagg cgccgccgcc gaaggtctcg gtgagcgcgt agccctgttg gacggtgaag
    25381 ccgagggcgt agaaatcacg aatgattttc gggtcgaaat aagaagcggc gctgatgagt
    25441 aagcgcagat ggccgccgaa agtctggtgt acgcgggaga aaatctttcg gcccatgttc
    25501 agacccgtcg cctgtctgag caggaacgac agacgcagca aggcccggaa gatgagccgg
    25561 acgggccggg gcttgcgccc gacctcctcg aagatttttt tatgaagcag gtagaagacc
    25621 tgcggcacgg ccgggaagat ggtgacacga gcgcgctgca aagtcttcag gagttcgacg
    25681 ctgttgagcg cgcgcaggta ggtgacgccc gcgcccgtcg ccagcggcat caggatgttg
    25741 gccagcgagg cgaagacgtg atgcgccggg acaacggcga ggacgttgtc gccgggaatg
    25801 aactcgatgt actgcttcag cgacaagacg ccggcggcca cggccccgtg ctcgatgacc
    25861 acccctttgg gcttccccgt ggtgccggac gtgtagaaga t
//
LOCUS       DASVES010000452         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_475384, whole genome shotgun sequence.
ACCESSION   DASVES010000452 DASVES010000000
VERSION     DASVES010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475384"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1092
                     /locus_tag="VF240_18785"
     CDS             271..1092
                     /locus_tag="VF240_18785"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7177311.1"
                     /translation="MPKKTMCVAALIIAAFLNSSCNTAQREPAADILSGRLVDLSHSF
                     DEQTVYWPTAEKTFKLERDAEGVTEAGYFYAANSFSTAEHGGTHIDAPIHFAQGRHTV
                     DQIPLEQLTGRAVVVDVAAKCAADADYQVSVEDFAAWEREHGALPQQGVIVLLRTGFG
                     SRWPERRSYMGTDERGAAAVAKLHFPGLHPDAARWLTQERRVKAVGLDTPSIDYGQST
                     LFESHRILFDKNVPAFENLANLDQLPAKDFHVIALPMKIRGGSGGPLRAIAIINR"
     gene            complement(1342..1890)
                     /locus_tag="VF240_18790"
     CDS             complement(1342..1890)
                     /locus_tag="VF240_18790"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7177312.1"
                     /translation="MHCPQCGQQQVSDEVRFCSRCGLSLAAVARLIEGGGRLAEFGEG
                     EGRVLSKRQRGVRKGLIVMAGSFVSCGLAGLLTAMDDDFFPLLLLASFAFIIGLMRML
                     YGMLLEDDAPQATATKKAAASKTPPALEGTRTHGAGAALPPARGFSAADLARPRNQTA
                     EMAAPSSVTESTTRLLENEERQ"
     gene            complement(2013..3182)
                     /locus_tag="VF240_18795"
     CDS             complement(2013..3182)
                     /locus_tag="VF240_18795"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX7177313.1"
                     /translation="MSQSGAAGTSLNKLIERLAGFEPTTLPFISLYLNAQANDTGKSD
                     FDRFVRKEFSERAKTYAPNTPERESFDRDAERITKYLEDVRPSANGVAVFACAGHEDF
                     FQAAQLDAPIEDHRLYVYNQPHLYPLARLAEQYRRYAVLVCDTNRGRIFVFGHGRTVE
                     SEEIENVKTNRTKVGGWSQMRYQRHAVNYHMQHAKEMVETLAGIVDKEGIEQVILAGD
                     EVIIPILRAQLPKALEDKVIDVLSLDIRAPEKEVFVASLEAMREHDAQTDAERVERLL
                     NEFRGGGLGVVGATDTLEALSMGQVDELYLTAAMREVKGDREDVEILSDTEVVEPVVA
                     ASDPAAVSNTEPDSVIVAQELVARAQQTSAKVFFIEDPALLAEYGGVGALLRYRI"
BASE COUNT          572 a         1087 c         1086 g          622 t
ORIGIN      
        1 cgcgcttcaa gagggagaag gcgctttcaa ccgtattcga tgaatagcac cgtccacgta
       61 gatgtcttca ctgtgcttga gggtcttgtg gttgaggccg ccgacggcct tgcctttggt
      121 gcgcgggtac acggggccgt caggctagcg caggcgcttt aataactcgc ggcacttttc
      181 gtcagtggag aacagcgtat gaacgctttc aagcgtcctt cagcttctcg cgctctcgta
      241 ctgcatgcga cccgacagga gggagacaga atgccgaaaa aaaccatgtg cgtcgcggcc
      301 cttattatag ccgcgttttt aaactcctct tgcaacaccg cgcagcgcga gcctgcggcc
      361 gacatccttt cgggccggct cgtcgatctc tcgcactcct tcgacgagca gacggtctac
      421 tggccgaccg ccgagaagac tttcaagttg gaacgagacg ccgagggcgt gacggaggcg
      481 ggatattttt acgcggccaa cagcttctca accgccgagc acggcggcac gcacatcgac
      541 gcgcccatcc acttcgccca aggccgacac acggtcgatc agattccgct cgaacagttg
      601 acgggccgcg ccgtcgtcgt ggacgtggcg gcgaagtgcg ccgccgacgc cgactatcag
      661 gtgtcggtcg aagacttcgc cgcgtgggag cgcgagcacg gcgcgcttcc ccaacaggga
      721 gtgatcgtgc tcctgcgtac aggcttcggg agccgctggc ctgagcgccg cagctacatg
      781 ggcacggatg agcgcggggc cgcggccgtc gcgaagctcc actttcccgg cctccatcca
      841 gacgccgcgc gctggctcac gcaagagcgc cgggtcaagg ccgtgggact ggatacgccg
      901 agcatcgatt acggccagtc aacactcttc gagagccacc ggattctttt cgacaaaaac
      961 gtccccgcct tcgagaacct cgccaacctc gaccagctcc ccgcgaaaga cttccacgtc
     1021 atcgcgctgc cgatgaaaat caggggcggc agcggcggcc cgctccgcgc catcgccatc
     1081 atcaacaggt gaaccgtcgg ccgaattcaa tgagccgctg taagcggcgg cagaatgtag
     1141 cccacggcgt gagccgtggg aaccaagcgc cggaaaaaca agaagccccg gaggggcgaa
     1201 agaaaatcga cgggcgcgat gctcgtccct gaaattcttt cgcccctccg gggcttcgct
     1261 gttctaatcc ggctcgttcc cacggctcgc gccgtgggct acattctttc gcccctgccg
     1321 gagcttatgg ccgcgacggc cttactgccg ttcctcgttt tcgagcagcc gcgtcgtgct
     1381 ctccgtgacg ctcgacggcg cggccatctc ggccgtctgg ttgcgcgggc gggcgaggtc
     1441 ggccgcggag aagccgcgcg ccggtggaag cgccgctccg gcgccgtgcg tcctcgtccc
     1501 ttcgagcgcg ggcggagtct tcgacgcggc ggctttcttg gtggccgtcg cctgcggcgc
     1561 gtcgtcttcg agcagcatgc cgtagagcat ccgcatgagg ccgatgatga aagcgaacga
     1621 ggccagcagc aacagcggga aaaagtcgtc atccatcgcc gtgagcagcc ccgcgagtcc
     1681 gcaggagacg aagctgccgg ccatgacgat caacccctta cgcacgccgc gctggcgctt
     1741 cgagagcacg cggccctcgc cttcgccgaa ttccgcgagg cggccgccgc cctcgatgag
     1801 acgcgcgacc gccgcgagcg acagcccgca acgcgaacag aagcgcactt cgtcggagac
     1861 ctgttgctgg ccgcactgcg ggcaatgcat cgtttcgccg tccttttggg gaagagtccg
     1921 aagcagtgag cagtgagcag taagcagtga gcagtcagga agtgtcgcca gctttgactg
     1981 ctcactgctt actgctcact gctcaccgct tttcagattc ggtagcgcag gagcgcgccg
     2041 acgccgccgt attcggcgag cagcgccggg tcttcgatga agaagacttt ggccgaggtc
     2101 tgctgcgcgc gcgcgaccaa ctcttgggcg acgatgaccg agtcgggttc ggtgttggag
     2161 acggccgccg ggtcgctcgc ggcgacgacc ggctcgacga cctccgtgtc cgagagtatc
     2221 tccacgtcct cgcggtcgcc cttcacctcg cgcatcgcgg ccgtcaggta gagttcgtcc
     2281 acctgcccca tcgagagcgc ttcgagcgtg tcggttgcgc cgactacgcc gaggcccccg
     2341 ccgcgaaact cgtttagcag acgctcgacg cgctcggcgt ccgtctgcgc gtcgtgctca
     2401 cgcatcgctt cgaggctcgc cacgaagacc tccttctcgg gcgcgcggat gtcgaggctg
     2461 agcacgtcga tcaccttatc ttcgagcgcc ttcggaagct gcgcgcgcag gatggggatg
     2521 atcacctcgt cgcccgcgag gatgacctgc tcgatgcctt ctttatcgac gatcccggcg
     2581 agcgtctcga ccatctcctt ggcgtgctgc atgtgatagt tcacggcgtg gcgctgatag
     2641 cgcatctgcg accagccgcc gaccttcgtc cggttcgtct tgacgttctc gatctcctcc
     2701 gactcgacgg tgcggccgtg gccgaagacg aagatgcgcc cgcggttcgt gtcgcagacg
     2761 agcacggcgt agcgccggta ctgttcggcg aggcgggcga gcgggtagag gtgcggctgg
     2821 ttgtagacgt agagccggtg gtcttcgatg ggcgcgtcga gctgcgcggc ctgaaagaaa
     2881 tcttcgtggc cggcgcaggc gaaaacggcg acgccgttcg ccgaagggcg cacgtcttcc
     2941 agatacttcg tgatgcgctc ggcgtcgcgg tcgaagctct cgcgctcggg tgtgttcggc
     3001 gcgtaggtct tggcgcgctc ggagaattcc ttgcggacga agcggtcgaa atcgctcttg
     3061 cccgtgtcgt tggcctgcgc gttcaggtag aggctgatga agggaagcgt cgtcggctcg
     3121 aacccggcga ggcgttcgat caacttgttc aaagacgtgc cggctgcacc actctgtgac
     3181 atctgtcgtg taccccttta gaagattttt tagactgctc tcaacaaact tgcggccttc
     3241 gggcgcgcac gctgccgcac tcgcttcggc cggcgcagcg gcgacccgtt gaaccgtcgg
     3301 gggaaggttt tggggaaatt tcgggaggat attagcacgc gccgccgggg cgttccaatc
     3361 cgtgccg
//
LOCUS       DASVES010000453        13312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1467843, whole genome shotgun sequence.
ACCESSION   DASVES010000453 DASVES010000000
VERSION     DASVES010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467843"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VF240_18800"
     CDS             complement(<1..1918)
                     /locus_tag="VF240_18800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7177314.1"
                     /translation="MSAATNPTRWQRLTDPYRRLVVAAGAAIFLHSALNLPVARLDFG
                     FLLLFLITVAISSRVSVPLPRVNATVTVSDTFIFLTLLLYGTEAAVALAAVEGVCAGM
                     RLSSRPLTVLCNSGALVCATFISGTLARLAFGQVTDFPRLPLSTAVSFACLLALAQYA
                     AHTGLGAAYLALKESLPLWRVWAKHYLWASVTYFVGGLLAGVLAKLTQAVSFYSLIFP
                     LPVIAIVYISYRKYSEDVRSTAAQAERAERERAEAERQRAESERERAEQAERHVEELN
                     HYIAKLESAQDELRESKEHFRHAAFHDALTGLPNRLLFTDHLRLAVERAKRDARYSFA
                     VLFLDLDRFKNINDSLGHTYGDELLVGIARRLQSCTRRVDTVARFGGDEFAVLLDGTG
                     GPEMAVRVAEKIQLELHTPISLHGHEAFTTASVGIALSCTGYGNPEDVLRDADTAMYR
                     AKDGGKARHQVFDRTMHTRAVTMLRLENDLRRALERGELRAYYQPIMSLRTGGLEGFE
                     ALVRWEHPDRGMVSPSEFIPLAEETGLIVPLGLWMLEEACRQLRAWQAISPANRGLTM
                     SVNLSGKQLAQADIAERVQDVLRETGLDPRSLKLEITESVVMENAEATAAVLARLRVL
                     GVGLAIDDFGTGYSS"
     gene            complement(1932..2774)
                     /locus_tag="VF240_18805"
     CDS             complement(1932..2774)
                     /locus_tag="VF240_18805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7177315.1"
                     /translation="MHQTQQMRVSPLAQSDGPEALSFLSERPVDNVIMSGFIRDNGVV
                     SPRNRGRFFGCRNRQGALEGVALVGHGMSFDSRSEAATELFAALARRSPESHLLMGES
                     GQVRSFWGYYAPGGAAPRKLREVTILEQRRPFEGCEQAPDLRPALSEELQQVAALHAE
                     MVLEETGDDPLRTEPESFRLRCLRRIERGRTWVWSEGGRIVYKADVIAQTPEVAYLEG
                     VYVSPRERGKGYGRRCLAQMGRRLLTRVTSVCLFVDEENERARDFYLSTGYAPSSRYN
                     ILYF"
     gene            complement(2901..4853)
                     /locus_tag="VF240_18810"
     CDS             complement(2901..4853)
                     /locus_tag="VF240_18810"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7177316.1"
                     /translation="MKPRRTTTISFIAVAALLLTLCPSGFVWGPADAADKPLKQETRG
                     ATKVKVSPDLRERLTAAQSDEQSVEVVLQLSGKPSGPLNALLKRAGVRLKGSFDSFDS
                     LAVSLPAGMVEELASFDEVEVVCPDREVERLGFIEQATGAAEMRRQSGNSGFKGKDMN
                     IAVLDSGVDKEHHGIGSRIELLKDFTGENRTDDPYGHGTHVASLAAGTDHVANGAYVS
                     PAPEAEIISLRVLNAQGVGLTSNVLRALQWILAPVDPTKPLGEKNHQKYRIRVVNISL
                     GAPAVDSYKVDPLCLAVSRLVAAGIVVVAAAGNDGADAAGRKIYGRIHAPGNEPSAIT
                     VGASNTFGTQSRADDGLTTFSSRGPTRSYSVVGGVKQYDHVIKPDLVAPGNKVIAAQA
                     RLNNIVRQHPGLGCTPGSGDSHKMMALSGTSMATPLVAGAVSVLLQANPRLTPNMVKM
                     ILQYTAQPLAGSNMLEQGAGQLNVEGAMRVAKAVRTDLSILTPPGAPLLVTSAPPMPF
                     STIDGYTFPWAQGILQNRSYATGTNLITQYQRAYGLGIAFGDGIAFGDGIAFGDLIMT
                     SNGMPIGTGIAFGDGIAFGDGIAFGDGIAFGDGIAFGDGILMGDGILMGDNRVDGDAA
                     AWSSYVMSNGDGTGAMPVVYEDGAAF"
     gene            complement(5081..5941)
                     /locus_tag="VF240_18815"
     CDS             complement(5081..5941)
                     /locus_tag="VF240_18815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX7177317.1"
                     /translation="MLQSAPMQPFSNLVPDSALIQDAIQLLRTNGGRATAVHLAEHVF
                     QLPGLEPETAVALVSELIKDDWRLKVADGSAEIEFAGEDDERRRLGETDYVVFDVETT
                     GPKMPPGRLLELGACRIQSGRIVGEFQTLLNPGTTIPPFISRLTGITDAMVRDSPTFD
                     EVAPDWLRFADTAVLVAHDAHFDVRFINHEVTLVYPGRRMFNAYLCTVSLARRLFPEL
                     GFYRLHALAKYFAVPLPNHHRAAADARATAEIFLHLLALLGSHGVRDISAARNFKLKT
                     RRSDRAATVN"
     gene            5985..6569
                     /gene="pyrE"
                     /locus_tag="VF240_18820"
     CDS             5985..6569
                     /gene="pyrE"
                     /locus_tag="VF240_18820"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7177318.1"
                     /translation="MLGAGDVLESFRRTGALLEGHFVLTSGLHSTVYLQCALVLQHPP
                     EAERFGRAIAEHFRNQRVETVVAPAIGGIVIGWEVARALNVRSLWTERVEGKMSLRRG
                     FHVRAGESVVVVEDVVTTGGSTRETVDALREAGARVVGAASIIDRSGGRADVGVPRVA
                     LVTLEVPAVAPAVCTQCAQGIPAVKPGSRKTSEQ"
     gene            6671..7420
                     /gene="truA"
                     /locus_tag="VF240_18825"
     CDS             6671..7420
                     /gene="truA"
                     /locus_tag="VF240_18825"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7177319.1"
                     /translation="MIHRLTIQYDGTEFHGWQMQDGPRTVQGELTRVLSLIEGRELTV
                     HGAGRTDAGVHAEAQVASVDLNREMTPERLRTAINANLARDVRVIDVRTAPDDFHARF
                     SARGKTYCYRVFNEQFTSPFWSRYAHAESRPLDLAGMRAAASLFIGEHDWTAFSAAQA
                     DVSSRVRTVRRLEITERQSRRGRGRMLEIWIGADGFLRYMVRSIAGALLAAGRGEMEG
                     QIIARAIETGERALAAPTAPANGLTLVEVHY"
     gene            7518..8309
                     /locus_tag="VF240_18830"
     CDS             7518..8309
                     /locus_tag="VF240_18830"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX7177320.1"
                     /translation="MLTDFAKVVVEGTRDEALLARVRWDRLPRHVAIIMDGNGRWANS
                     RGLPRVAGHRAGVDAVRAAVDTGARLGLGALTLYAFSTENWKRPRLEVDALMRMLKRY
                     LRLELEEIDRQNIRFQPVGRVEALAESVRRELDRSVERTAHNTGMVLSVALNYGGRAE
                     IVDAVNAAVRRQQAEGGAGLELTEESIGRELYTCDLPELDLLIRTSGELRISNFLLWQ
                     IAYSEIYVTETLWPDFRRVDMLEAIVDFQQRNRRFGGLQLVAKAK"
     gene            8455..9309
                     /locus_tag="VF240_18835"
     CDS             8455..9309
                     /locus_tag="VF240_18835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7177321.1"
                     /translation="MKRLITAAIALPILIASIIVPQLHLLFVALAVGAVFVALYEFWF
                     LAKRLGGRPDVVVGYAATAAILASFYFNEPVWLLIILPVFVAAALVAEMLRGAPFDKM
                     ILSAGSTVLGVLYIALLGGHLIGVRTGFALSLSPAPDSYTLSTHLLSFFFLVLMGSDT
                     GAYYTGRALGRRKLAPNISPGKTWEGAAGGMAASLLMATLAHYWFFPELPLRAALPLA
                     AVMNVLGVVGDLVESALKRGSNAKDAANILPGHGGFLDRLDSLLLGAPLLYYFAVLFW
                     RESATIIR"
     gene            9377..10579
                     /locus_tag="VF240_18840"
     CDS             9377..10579
                     /locus_tag="VF240_18840"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7177322.1"
                     /translation="MIPQGIAILGSTGSIGCNTLRVVETLDAGERFRVVALAAGRNVA
                     RLAEQIERHRPELVAVESAERAEELHAELARRDVPPPARVLTGEAGLVEVATHAEASC
                     VVSATVGAVGLVPTLRALEAGKRVALANKEPLVMAGELMTRAARASGAELLPVDSEHN
                     ALHQCLRGERPEEVRRLVLTASGGPFRSKSAAQMEAATVEEAMRHPTWNMGAKITIDS
                     ATLMNKGLEVIEAHWLFDFGPDAIDVIVHPESIVHSMVELADGSVIAQMGVTDMRHAI
                     QYALTYPERLPGDLPALDLTRLGALHFEAPDFGRFPCLGLAYRALREGGTQPAALNAA
                     NEEAVAAFLDSRLRLTDIARVNESVLDGNESRPAESLSAVLDADAKARVRARAEISRL
                     RGAERVVA"
     gene            10701..12089
                     /gene="rseP"
                     /locus_tag="VF240_18845"
     CDS             10701..12089
                     /gene="rseP"
                     /locus_tag="VF240_18845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX7177323.1"
                     /translation="MFFYLSFIVILGAAVIIHEFGHFIVAKLLGIRVETFSVGFGKRL
                     FGRKWGTTDYRVSLIPLGGYVKLGGDDSNAGIEEGSDDSANVPVKERFDLRPRWQKFL
                     VAVAGPIMNILTAIAIPLAGAMAVGAPVMPPPVVKTVQQGAAGEAAGLRPGDRIVSFN
                     GRDDSDWERISNDALLSPEQPIPLVVERDGERVPLTIRPVKKLSEGEAYGELGIVPDH
                     GDVPVVIFSVEKDTPASEAGILPGDRVVSIGGEQVGSAAQIIQYINEHPRQPITMQIE
                     RDGQTREVTATARRLSDGRERLGIGPGEAAPIRKLGLTDALGYSVGQNIEILRLTGMA
                     LKQVFTGQRSMRDTFSGPVGIAVASKRAVEEGGVEGGLKMLGFLSLNLGVFNLLPIPV
                     LDGGMIFMLLIEGILAIVGMKLSMGMRERIQQVGFVFLLLLMSFVIINDLAKVASRWR
                     GAESPPAATEQK"
     gene            12188..13297
                     /gene="ispG"
                     /locus_tag="VF240_18850"
                     /gene_synonym="gcpE"
     CDS             12188..13297
                     /gene="ispG"
                     /locus_tag="VF240_18850"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX7177324.1"
                     /translation="MRRETRAVRVKDVQIGGGAPVAVQSMTKTETTDVEGTVRQIEEM
                     VRAGCEIVRVAVPDQPAALALKEIRKRVPDVPLVADIHFHYKLALLALEAGVDKLRLN
                     PGNIGAHERVREVVRAAAAQGVPIRIGVNGGSLEKDLLKRYGTATPEAMVESAERHIQ
                     ILEDLDFRDIIVSLKASDVHRTVAAYRLLSAKVDYPLHLGVTEAGTAFGGTIKSAMGL
                     GILLHEGIGDTIRVSLAAEPQEEVRVAWEVLKSLELRKRGVTVVACPTCGRLDVDNFV
                     EIVTEIERRLAHIEEPLHLSIMGCEVNGPGEAHDSQLGVTFGRGVGMIFKNGRPMRKV
                     AGTDIVEAFVAETEKLLAEGAQEPEEAQPELVQIT"
BASE COUNT         2213 a         4314 c         4521 g         2264 t
ORIGIN      
        1 agctggagta gccggtgccg aagtcgtcga tggcgagacc gacgccgagc acgcgcaggc
       61 gggcgaggac ggcggcggtc gcctcggcgt tttccatcac cacgctctcc gtgatctcca
      121 gcttgagcga gcgcgggtca agcccggtct cgcgtagcac gtcctgcacc cgctcggcga
      181 tgtcggcctg cgccagttgc ttgcccgaaa ggttcacgct catcgtcagc ccgcggttgg
      241 cgggcgagat ggcctgccac gcgcgcaact gtcggcacgc ctcctccagc atccagaggc
      301 cgaggggcac gatcaacccg gtctcctcgg cgagcgggat gaactcggac ggcgagacca
      361 tcccccggtc agggtgctcc caacggacga gcgcctcgaa cccttcaagc ccgcccgtcc
      421 gcagcgacat gatcggctgg tagtaggcgc gtaactcccc gcgttcaagg gcccgccgca
      481 agtcgttctc cagccggagc atggtcacag cccgcgtgtg catcgtgcgg tcgaagacct
      541 ggtgccgcgc cttgcccccg tccttcgcac ggtacatggc cgtgtccgcg tcccgcagca
      601 cgtcctcggg gttgccgtag ccggtgcaac tgagggcgat gccgacgctg gccgtcgtga
      661 acgcctcgtg gccgtggagg ctgatcggag tgtgtagctc aagctggatt ttctcggcca
      721 cccggacggc catctcgggg ccgccggtgc cgtcgagcag gacggcgaac tcgtcgccgc
      781 cgaagcgcgc gacggtgtcc acccggcggg tgcatgactg gagccggcgc gcgattccga
      841 cgagcagttc gtcgccgtag gtgtggccga gcgagtcgtt gatgttcttg aagcggtcga
      901 ggtcgaggaa gagcacggcg aacgagtagc gggcgtccct cttggcgcgc tcgacggcga
      961 gtcgcaggtg gtctgtgaac aacaggcggt tgggcagccc ggttagggcg tcgtggaagg
     1021 cggcgtggcg gaagtgctcc ttgctctccc gcagctcgtc ctgggcgctc tccagtttgg
     1081 cgatgtagtg gttgagctcc tcgacgtggc gctcggcctg ctcggcccgc tcgcgctccg
     1141 actccgcccg ctgccgctcg gcctcggccc tctcgcgctc ggcccgctcg gcctgagcgg
     1201 ccgtcgagcg cacgtcctcc gaatacttcc ggtaggagat gtagacgatg gcgatgacgg
     1261 ggagggggaa gatgagcgag tagaagctga cggcctgcgt gagcttcgcc aggacgcccg
     1321 cgagtagccc cccgacgaaa taggtcacag aagcccagag ataatgtttc gcccacacgc
     1381 gccaaagggg caggctctct ttcagcgcga gataggccgc gccgaggccg gtgtgcgcgg
     1441 cgtactgcgc aagggcgagc aggcaggcga agctgacggc ggtcgagagc ggcaggcgtg
     1501 ggaagtccgt cacctgcccg aaggcgaggc gggccagtgt gcccgagatg aaggtcgcgc
     1561 agaccagcgc gcccgagttg cacaggacgg tgagcggccg actgctcaga cgcatcccgg
     1621 cgcagactcc ttcgaccgcc gccagcgcga ccgccgcctc agtcccgtag aggaggagcg
     1681 tcaggaagat gaacgtgtcc gagaccgtga cggtcgcgtt gacgcggggg agtggcaccg
     1741 agacccgcga gctgatcgcc accgtaatca ggaacaggag caggaaaccg aagtccaggc
     1801 gggccacggg taggttgagc gccgagtgga ggaagatcgc cgcccccgcc gcgacgacga
     1861 gtcgccggta cgggtcggtc aggcgctgcc agcgcgtcgg gttcgtggcg gcactcaaga
     1921 gattcgttgg actaaaagta gaggatgttg taacgactgg aaggggcgta accggtgctg
     1981 aggtagaaat cccgtgcgcg ttcgttctcc tcgtccacga acaggcagac cgaagtgacg
     2041 cgggtcaaaa gccgccggcc catctgggcc aggcagcggc gcccgtaccc cttcccgcgc
     2101 tcccgcgggc tgacgtacac gccctcgagg taggcgacct ccggcgtctg cgcaatcacg
     2161 tcggccttgt agacaatgcg cccgccctct gaccagaccc atgtcctgcc ccgctctatg
     2221 cgccgcaggc agcggaggcg gaagctctcc ggctccgtgc gcaacgggtc gtcgccggtt
     2281 tcttcaagca ccatctcggc gtggagcgcc gccacctgtt gaagctcttc cgacaaggcc
     2341 gggcgcagat cgggcgcctg ctcacacccc tcgaaggggc ggcgctgctc aaggatggtg
     2401 acctcgcgca acttccgggg cgcggcccct ccgggcgcgt agtagcccca gaagctccgc
     2461 acctgtccgc tctcgcccat cagcaggtgc gactcaggcg acctccgggc gagggcggcg
     2521 aacagttccg tcgcggcctc tgagcgcgag tcgaaagaca ttccgtgccc gacgagggcg
     2581 acgccttcga gcgcgccctg tcggttccga cagccgaaga accggccccg gttccgggga
     2641 ctcaccacgc cgttgtcgcg gatgaagccg gacatgatga cgttgtccac cggccgctcc
     2701 gagaggaacg agagcgcctc ggggccgtca ctctgtgcga gtgggctgac tctcatctgc
     2761 tgcgtctggt gcacggtttg accttcagtg tttacgtcgg cgtggcccct gcttcaacag
     2821 aagggccggc cgcgttaccg ttacagttgt cgcgccgaca atttgcggct tcgcgtgtgg
     2881 cgggccgcaa gcgtgtcggt ttagaacgcg gcgccgtcct cgtacaccac gggcatcgcg
     2941 cccgtgccgt cgccgttcga catgacgtaa gacgaccacg cggccgcgtc tccatcgaca
     3001 cggttgtcgc ccatgaggat gccgtcgccc atgaggatgc cgtcgccgaa ggcgatgccg
     3061 tcgccgaaag ctattccatc gccgaaagct atcccatccc cgaacgcgat gccgtcgccg
     3121 aaggcgatgc ccgtcccgat gggcatcccg ttgctcgtca tgatgagatc gccgaaggcg
     3181 atgccgtcgc cgaaggcgat gccatcaccg aaggcgatgc cgaggccgta ggcgcgctgg
     3241 tactgcgtga tgaggttcgt gccggtcgcg taactgcggt tctggagaat gccctgagcc
     3301 caggggaagg tgtagccgtc gatggtagag aagggcatgg gcggcgcgga ggtcacgagg
     3361 agcggcgcgc cgggcggggt caggatcgac aggtcggtgc ggacggcttt ggcgacccgc
     3421 atcgcgccct cgacgttgag ctgcccggcc ccctgttcga gcatgttgga gcccgcgagc
     3481 ggctgcgccg tgtactggag aatcattttc accatgttcg gcgtgagccg ggggttggcc
     3541 tggagcagca ccgagacggc gcccgcgacg agcggcgtcg ccatcgacgt gccgctcaga
     3601 gccatcatct tgtggctgtc gccgctcccc ggcgtgcaac ccagaccggg gtgctgccgc
     3661 acgatgttgt tgagccgggc ctgggccgcg atcaccttgt tgccgggggc gacgaggtcg
     3721 ggcttgatga cgtggtcgta ctgcttcacc ccgcccacca cactgtaact gcgcgtcggc
     3781 ccgcgcgagc tgaaggtggt gaggccgtcg tcggcgcgcg actgcgtgcc gaaggtgttt
     3841 gaagcgccga ccgtgatggc cgacggctcg ttgcccggcg cgtggatgcg gccgtagatt
     3901 ttgcgccccg ccgcgtccgc cccgtcattg ccggcagccg cgacgaccac gatcccggca
     3961 gcgacgagcc tgctcacggc caggcagagc gggtctacct tgtaagagtc cacggccggg
     4021 gcgccgaggc tgatgttgac cacgcgaatc cggtacttct ggtgattctt ttcgccgagc
     4081 ggcttagtgg gatcgaccgg cgcgagaatc cactggaggg cgcgcagcac gttcgaggtc
     4141 agtccgactc cctgcgcgtt gagcacgcgg aggctgatga tttccgcttc gggggccggg
     4201 ctgacgtagg ccccgttcgc gacgtggtcg gtgccggcgg ccagcgaagc gacgtgagtg
     4261 ccgtgcccgt aggggtcgtc cgtgcggttc tcgccggtga agtctttcag gagttcgatg
     4321 cggctgccga tgccgtggtg ctccttgtcc acgcccgagt cgagcacggc gatgttcatg
     4381 tccttgccct tgaagccgct gttgcccgac tgccgccgca tctccgcggc gcccgtcgcc
     4441 tgctcgatga agcccagcct ctccacttca cggtctgggc agacgacttc cacctcatcg
     4501 aaagatgcca gctcttcgac catgccggcg ggtaggctca ccgccagcga gtcgaagctg
     4561 tcgaagctgc ccttcagccg cacgccggcg cgtttgagga gtgcgttgag cgggccgctc
     4621 ggcttaccgc tgagctgtag cacgacttca actgactgct cgtcggactg tgcggccgtc
     4681 agacgctcgc gcaggtcggg cgaaactttg actttcgttg cgccgcgcgt ttcctgcttc
     4741 aaaggcttgt cggcggcgtc ggccggcccc cacacgaaac cgctggggca gagcgtcagt
     4801 agcagggcgg ccacggcaat gaaggagatg gtcgttgttc tacgaggttt catttcggcc
     4861 tcatctggat tgacatgttt ccgcggcggg atatccgtgc ggataaaagg atattggcaa
     4921 cttcggtgcc gcattgtttc cacagcacga agcgcgtagt cgtgggaaaa tttgagggaa
     4981 ggcggtgtcg cgacgaacga gaaatgctca aacggaaatg tcagagtgac gtgggacggt
     5041 cagactgacc acccgaggaa ccatacgcgc gccgacgcca tcagttgacg gtcgccgccc
     5101 ggtcgctgcg gcgggtcttg agtttgaagt tgcgcgcggc ggagatgtcg cgcacgccgt
     5161 ggctgccgag gagggcgagg aggtggagaa agatttcggc ggtggcgcgg gcgtcggcgg
     5221 cggcgcggtg atggttgggg agggggacgg cgaaatattt ggcgagagcg tgcaggcggt
     5281 agaagccgag ttcggggaag agccggcgcg cgagcgagac ggtgcagaga taggcgttga
     5341 acatgcgccg ccccggatag acgagcgtga cctcgtggtt gataaaacgc acgtcgaagt
     5401 gcgcgtcgtg cgcgacgagc acggccgtgt cggcgaagcg gagccagtcg ggggcgacct
     5461 cgtcgaaggt gggcgagtct ctgaccatcg cgtcggtgat gccggtcaga cggctaatga
     5521 agggcgggat ggtcgtgccg gggttgagca gcgtctggaa ctcgccgacg atgcggccgg
     5581 attggatgcg acacgcgccg agttcgagga gacggccggg cggcatcttc gggccggtgg
     5641 tctccacgtc gaagacgacg taatcggtct cgccaaggcg tcggcgctcg tcgtcctcgc
     5701 cggcgaactc gatctcggcc gagccgtcgg cgaccttcag acgccagtcg tccttgatga
     5761 gttcggagac gagtgccacg gccgtctccg gctcaaggcc cggcagttgg aagacgtgct
     5821 cggcgagatg gacggccgtg gcgcgcccgc cgttcgtccg caggagttga atcgcatctt
     5881 gaatgagcgc agaatcggga accagattgc tgaagggctg catcggggcg gattgtagca
     5941 cgccgacttt agttcgagaa taagcatcgg ggaggacggc cgctatgttg ggcgcgggcg
     6001 atgttctgga aagtttccgc cgcacgggcg cgctgctcga aggccacttc gtgttgacga
     6061 gcgggctgca cagcaccgtc tacctgcaat gcgcgctcgt cctccagcac ccgcccgagg
     6121 ccgaacggtt cggccgcgcc atcgccgagc acttccgcaa tcagcgcgtc gagacggtcg
     6181 tcgcgccggc catcggcggc atcgtcatcg ggtgggaggt ggcgcgcgcc ctcaatgtgc
     6241 gctcgttgtg gacggagcgc gtggaaggga agatgtcgct ccggcgtggc ttccacgtgc
     6301 gcgcaggtga gagcgtcgtc gtggtggagg acgtggtcac gaccggcggc tcgacgcgcg
     6361 agaccgttga cgcgctgcgc gaggccggcg cgcgcgtcgt cggcgcggcc tccatcatcg
     6421 accgctcggg tgggcgggcc gacgtgggcg tcccgcgcgt ggcgctcgtc accctcgaag
     6481 tcccggccgt ggcccctgcc gtctgcaccc aatgcgctca gggcatcccg gccgtcaaac
     6541 ccggaagccg gaagacaagt gagcagtgag cagtaagcag tgaacagtaa aagacagact
     6601 cacgattcgg tgctacatct aattaatctg ctcactgctt actgctcact gctcactgct
     6661 tactgccctt atgatacatc gcctgacgat tcagtacgac ggcactgagt ttcacggttg
     6721 gcagatgcag gatgggccgc ggacggtgca gggcgagctg acgcgcgtgt tgtctctcat
     6781 cgaggggcgg gagctgacgg tgcacggcgc gggacggacg gacgcgggtg ttcacgccga
     6841 ggcgcaggtg gcgagtgtcg atttgaatcg cgagatgaca cctgagcggc ttcgcaccgc
     6901 gattaacgcg aacctcgcgc gcgacgtgcg ggtgattgac gtgcggacgg cccccgacga
     6961 ctttcacgcg cgcttctcgg cccgcggcaa aacctattgc taccgcgtct tcaacgaaca
     7021 attcacgtcg cccttctggt cgcggtacgc gcacgccgag tcgcgcccgc tcgacctcgc
     7081 agggatgcgc gccgccgcgt cgctcttcat cggcgaacac gactggacgg ccttctcggc
     7141 ggcgcaggcc gatgtcagct cgcgcgtgcg aaccgtcagg cgtttggaaa tcacggagag
     7201 gcaaagtcgg cgcggacgcg ggcgcatgtt ggaaatttgg atcggcgccg acggcttcct
     7261 ccgctacatg gtgcgctcca tcgccggcgc gttgctggcc gccgggcgcg gcgagatgga
     7321 ggggcagata atcgcgcgcg ccatcgagac gggggagcgt gcgctggccg ccccaacggc
     7381 gcccgccaac gggctgacgc tcgtcgaagt tcactactga ggcgctccga gggccgcaac
     7441 gactttgcac tttaaactgg tttacgatta acattcggcc ccggagactt tacggcttct
     7501 cggttcgagg ggactcgatg ctgacggatt tcgcgaaggt tgtggtggaa ggcacgcgcg
     7561 acgaggcgct tctggcgcgc gtccgctggg atcgactgcc gagacacgtc gccatcatca
     7621 tggacggcaa cggtcgctgg gccaactcgc gcggcctgcc gcgtgtcgcc ggccaccgcg
     7681 ccggagtcga tgccgtgcgc gccgccgtgg acaccggcgc gcgtctcggc ctcggcgcgc
     7741 tcacgctcta cgccttctcg accgagaact ggaagcgccc gcgtctggaa gttgacgcgc
     7801 tcatgcggat gctcaagcga tatctccgtc tggagcttga ggaaatcgac cgccagaaca
     7861 tccgcttcca gccggtcggg cgcgtcgagg cgctcgccga gagcgtccgc cgcgaactcg
     7921 accgctcggt cgagcgcacg gcccacaaca ccggcatggt cttgagcgtc gccctcaact
     7981 atggcggccg cgccgagatc gttgacgcgg tgaacgcggc cgtccggcgc caacaggccg
     8041 aaggcggcgc gggcctggag ctgaccgagg agtccatcgg gcgtgagcta tacacctgcg
     8101 atctgcccga actcgacctc ctcatccgca ccagcggcga gctgcgcatc tcgaacttcc
     8161 tgctctggca aatcgcctac agcgagattt atgtcaccga aacgctctgg cctgacttcc
     8221 gccgcgtgga catgctcgaa gcaatcgtcg atttccagca gcgcaaccga cgcttcggcg
     8281 gcctgcaact cgtggccaag gcgaagtgag gagagctgtt agctattagc cgttagcttt
     8341 cagctgttag cttttcctga tgtggaacgc cacacgtcct ttacaacatc aactttggcc
     8401 acacgcttca aacttcctgc taaccgctaa cagctaatag ctaacagccc cttcatgaaa
     8461 cgtctcatca cggccgccat cgcgctcccc atcctgatcg cctcgatcat cgtcccgcag
     8521 cttcatcttt tattcgtcgc gctcgccgtc ggggcggtgt tcgtcgcgct ctacgagttc
     8581 tggtttctcg ccaagcgtct gggcggaagg cccgacgtgg tggtgggcta cgcggcgacc
     8641 gccgcgatcc tcgcctcctt ctacttcaac gagccggtgt ggctcctcat catcctgcct
     8701 gtgttcgtcg ccgccgcgct cgtggcggag atgctgcgag gcgcgccgtt tgacaagatg
     8761 atcctgtcgg ccggctcgac cgtgttgggc gtgctctaca tcgcgctgct cggcgggcac
     8821 ctcatcgggg tgcggacggg cttcgcgctg agcctgtcgc cggcgccgga cagctacacg
     8881 ctctcgacac acctgctctc gttcttcttc ctcgtcctga tgggttccga caccggcgct
     8941 tactacaccg ggcgcgcgct cggccggcgc aagctcgccc cgaacatcag tccgggcaag
     9001 acgtgggaag gcgcggccgg cggcatggcc gcctcgcttt tgatggccac gctcgcgcac
     9061 tactggttct tccccgaact gccgctgcgc gccgcgctcc cgctcgcggc ggtgatgaac
     9121 gtgctcggcg tggtcggaga tttggtggag agcgctctta agcgcgggtc gaacgcaaag
     9181 gacgcggcca acatcctccc cggccacggc ggctttctcg accggctcga tagcttgctg
     9241 ttgggcgcgc cgctcctcta ctatttcgcc gtcctgttct ggagagaatc ggccacaatc
     9301 atccgctgag gcggaaggag agcggtgtgc cggcaagtta gacgcgaacg gagttggtcg
     9361 agatactcga aaaaatatga tcccgcaagg catcgcgata ctgggttcga caggctcgat
     9421 tggatgcaac accctgcgcg tcgtcgaaac actcgacgcg ggggagcggt tccgcgtcgt
     9481 cgcgctcgcc gccggccgca acgtcgcgcg tctcgccgaa cagattgaac gacacaggcc
     9541 cgaactcgtc gccgtcgaaa gcgccgagcg cgccgaagag ttgcacgcgg aactcgcccg
     9601 gcgagacgtg ccgccgcccg cgcgcgtcct caccggggag gcggggctgg tggaggtcgc
     9661 cacgcacgcg gaggcgtcct gcgtggtgag cgcgacggtc ggggcggtcg gcctcgtgcc
     9721 cacgctgcgc gcgctggagg ccggaaagcg cgtcgcgctc gccaacaagg agccgcttgt
     9781 gatggcgggc gagctgatga cgcgcgcggc gcgtgcgtcg ggcgccgagc tgctgccggt
     9841 cgattcggag cacaacgcgc tgcaccagtg cctgcgcggc gagcgcccgg aggaagtgcg
     9901 ccggcttgtg ctgacggcct cgggcggccc ttttcggtcg aagagcgcgg cccagatgga
     9961 ggccgccacg gtcgaagagg ccatgcgcca cccaacgtgg aacatgggcg cgaagatcac
    10021 catcgactcg gccacgctca tgaacaaagg actcgaagtg atcgaggccc actggctttt
    10081 cgacttcggc cccgacgcca tcgacgtgat cgtccacccc gagtcaatcg tccattcgat
    10141 ggtcgaactc gccgacggct ccgtcatcgc gcaaatgggt gtgaccgaca tgcgccacgc
    10201 catccaatat gcgctcacct atcccgagcg actccccggc gacctccccg cgctcgacct
    10261 gacgcggctc ggcgcgctcc acttcgaggc ccccgacttc ggacgcttcc cctgcctcgg
    10321 cctcgcctac cgcgccctgc gcgagggcgg cacacaaccc gccgcgctca acgccgccaa
    10381 cgaggaggcc gtcgccgcct tcctcgactc ccgcctgcgc ctgaccgaca tcgcacgcgt
    10441 caacgagagc gtgttggacg gcaatgagtc gcgcccggcc gaaagtttgt cggccgtcct
    10501 tgatgcggat gcgaaggccc gcgtgagggc ccgcgccgaa atctcccgtc tgcggggcgc
    10561 cgaaagagtg gtcgcgtgag ggggcggttt gacctgcgtc gtaaaagatt agagaatcgt
    10621 agtgcaaccg aagcggggcg ccggaggctc caagtcgtcg ttgtcgtgtg tttagacgtg
    10681 taagttaaga gaaggggttt atgttttttt atctgtcctt catcgtcatt ctgggcgcgg
    10741 cggtcatcat tcatgaattc ggtcacttca tcgtcgccaa gctgctcggc atccgcgtcg
    10801 agactttttc ggtggggttc ggtaagcgtc tcttcggcag gaagtggggc acgaccgact
    10861 atcgcgtcag cctgattccg ctgggcggct acgtcaagct cggcggcgac gactcgaacg
    10921 ccggcatcga agaaggctcc gacgactccg cgaacgtccc cgtcaaggaa cgtttcgacc
    10981 tgcggccgcg ctggcagaag tttctggtcg ccgtcgccgg ccccatcatg aatatcctga
    11041 cggccatcgc catcccgctc gcgggcgcga tggcggtggg cgcgcccgtg atgccgccgc
    11101 ccgtcgtcaa gaccgtgcag cagggcgccg ccggcgaggc cgcgggactg cgccccggcg
    11161 accgcatcgt ctccttcaac ggccgcgacg acagcgactg ggagcgcatc agcaacgacg
    11221 cgctgctctc gcccgaacag cccatcccgc tcgtcgtcga gcgcgacggc gagcgcgtcc
    11281 cgctcaccat ccgccccgtc aagaagctca gcgagggcga agcctacggc gaactcggca
    11341 tcgtccccga ccacggcgac gtgcccgtcg tcatcttcag cgtcgagaag gacacgcccg
    11401 cctccgaagc cggcattttg ccgggcgacc gcgtggtctc catcggcggc gagcaggttg
    11461 gcagcgccgc ccagatcatc cagtacatca acgagcaccc gcgccagccc atcacgatgc
    11521 aaatcgagcg cgacgggcag acgcgagaag tcacggccac cgcgcgccgc ctctcggacg
    11581 gacgcgagcg cctcggcatc ggccccggcg aggccgcgcc gattcgcaag ctcggcttaa
    11641 ccgacgcgct cggctactcg gtcggacaga acatcgaaat tctgcggctc accggcatgg
    11701 cgctcaagca ggtcttcacg gggcagcgtt cgatgcgcga caccttcagc ggccccgtcg
    11761 gcatcgccgt cgcctcgaag agggcggtgg aggagggcgg cgtcgagggc ggcctcaaga
    11821 tgctcggctt cctgagcctc aacctcggcg tcttcaacct gctgcccatc cccgtgctcg
    11881 acggcggcat gatctttatg ctgctcatcg agggtatcct cgcaatcgtg gggatgaagc
    11941 tctcgatggg gatgcgcgag cgcatccagc aggtcggctt tgtcttcctg ctcctgctga
    12001 tgagcttcgt catcatcaac gatctggcga aggtcgcctc gcgctggagg ggcgccgaaa
    12061 gcccgccggc cgcgacggag cagaaataaa gatgagttca aagttcaaag ttcaaagttc
    12121 aaagtcaaca gcagccaggc gcgctttgaa ctttgaactt tgaactttga acttcgttag
    12181 gatttccatg agacgagaga ccagagcggt tcgggtgaag gacgtgcaga tcggcggcgg
    12241 cgcgcccgtg gccgtccagt cgatgacgaa gacggagacg accgacgttg agggcaccgt
    12301 ccggcagatc gaagagatgg tgcgcgccgg ttgcgagatc gtgcgcgttg ccgtccccga
    12361 ccagccggcg gccctcgccc tcaaagagat tcgcaagcgc gtgcctgacg tgcccctcgt
    12421 cgccgacatc cacttccact acaagctcgc gttgctggct cttgaggcgg gcgtcgataa
    12481 gctccgtttg aaccccggca acatcggcgc tcacgagcgc gtgcgcgagg tggtgcgcgc
    12541 cgcggcggcg cagggcgtgc ccatccgcat cggcgtcaac ggcggctcgc tcgaaaaaga
    12601 tttgctcaag cgctacggca cggccacgcc cgaggcgatg gtcgagtccg ccgagcgcca
    12661 tatacagatt ctcgaagacc tcgacttccg cgacatcatc gtctccctca aggcttctga
    12721 cgtacaccgc acggtcgccg cctatcgcct gctctccgcg aaggttgact acccgctgca
    12781 cctcggcgtg accgaggcgg gaacggcctt cggcggcacg atcaagtcgg cgatgggcct
    12841 cggcatcctc ctgcacgaag gcatcggcga cacgatccgc gtctctttgg ccgcggagcc
    12901 gcaggaggag gtgcgcgtgg cgtgggaggt tctgaagtct ctggagcttc gcaagcgcgg
    12961 cgtgacggtc gtcgcctgcc cgacgtgcgg gcggctcgac gtggacaact tcgtcgagat
    13021 cgtcacggag atcgagcggc gactggcgca catcgaggag ccgttgcacc tctcgatcat
    13081 gggctgcgag gtcaacggcc ccggagaggc gcacgacagt cagctcggcg tcaccttcgg
    13141 acgcggcgtc gggatgatct tcaagaacgg ccggcccatg cggaaggtcg ccggcacgga
    13201 catcgtcgag gcgttcgtag ccgagacgga aaagctcctc gccgaaggcg cacaggagcc
    13261 cgaggaagcg cagccggaac tcgtgcagat aacctaagtg aagttggtaa gt
//
LOCUS       DASVES010000454        17538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1269988, whole genome shotgun sequence.
ACCESSION   DASVES010000454 DASVES010000000
VERSION     DASVES010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17538
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269988"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4760
                     /locus_tag="VF240_18855"
     CDS             <1..4760
                     /locus_tag="VF240_18855"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX7177325.1"
                     /translation="EQMQQRAKRPRRPEITPQFETDLRLLRRAGGQPLPGHLRAFLEP
                     RFGHNLGDVRVHVDHSAAELAQKANARAFTVGKHIVFAAGEYRPGVEQGRRLIAHELT
                     HVFQQRGGLHSVQREVGPERVATADPQADLPSLEELRAAFKLDAASAPPSILSVAVAL
                     LRNALRSTSNAQRLRPLTRSEGETAALVRAIQSGAYTLELASLRGGREKGWKLTQRTR
                     RETFVSRSNSATQQWPAATAGDDTITLPTPPAAEDYPQGTIVAGLESISPGDRVEGAK
                     PTPLIPKTVSEGAVPVRKSSLPTPVPETQTVPTAAPAAKVVAESVEAPAESLKEAAPA
                     ADAATAAAQPATSEAPAAEDGAPAAAEPEQAPLDPQKDPEFQQTLGQIKRTRKAQGAH
                     IPSDDKLQHTKDATVLKEEKQREKHDRDKHLTEIDATAKKTESVPFTPEEFKKLLNAS
                     IKKIETKLPRNEDDANRFKKERPLEQTKEEVRGQVKKKNDEIAGPLATQVDPEKPPPQ
                     AKPFDPKPTADLIEEEVGKRPHNISPAAAAPKPRFDSEISMEKESQSLDDLMSENEIT
                     EEQLAESNEPTFVEALGSKKAAQEKAAQAPAQFRAVEQPILDKSQAKAGQKGASKFGA
                     MFKGRDEAFGLVSGKQKLTVNDDKAKQEDFLIKLKGIYESTKTDVEGILDALSNTVDA
                     LFTLQVTLAKNLFESQVESQLDDIYGFTTWDDSLFGADTEAIEGVFRREKDRFLATMD
                     RIIDQIAKFIADMLNAAMARIKRGRKEADDLFASLSTEQQRLSTDAMGLFKTQFEMLE
                     DGVREKQQDLADTLAESYKSNVDSLRESFDKIKEDISTGWIGKAVNFIKSVAETIKKL
                     GELLLSILSRIGAIIGDILAHPIRFIENLAKGVGDGFDMFIERLDDYLIAGFFDWIRG
                     TVGGPGIDIPDKFDAAGIFSLVAQVAGLSYDTFRKVASKVWGKAAVEFIDQGKAVAEK
                     GLEIFFIVKEKGLGGLWEHIQEHLGSMVDDLMTKVKETVLYEVIKKALAYIAGLFNPV
                     GAFIKAAQAIYAGIRFLMDNIQRIADIVDAFLDSLELAVAGKTDLISQKIVTALRGVI
                     VIAIDFLAKLLGLGDLDEKVRKILKVIRTPVERAMEVVLKALRPLVQKVMRTLGVGKD
                     EEEAEKEVEKKGGEPQTHDEVLDHVVGLMAQETKADTPALALEEKKEQAQNLIKKYQP
                     LLKHGQLQILINDKNSEDVVEDSAVDFDVSASPGRKGQALVPIKVVDERAVQERFSHA
                     RSLKKFTDEKGFGLDDWAGTFSHLSHSTHLADMRYGLDSKVKLLTKQGDTYHFKADVK
                     DAEVVRLGALELQNEGRHHAPLQKGRFGVPLIQTFLSGRKIAGIPPNIFDDRAVVSKV
                     AAAAEGAGAIVKVGKTDTWALPGIQPQRLLPDNWRGSDNIRPRFYYKEAGFADLADEL
                     FEKVIKDEIVDLIEAQLENIEDARKLGLPPSQADMKPWETMKKREYADTNEKFSVGLA
                     RIDGYYQRNRYDVDHVTSLAKHWNNKGHDDGQTERIAATQGKRGGLALMEKGLNRSKG
                     SGGINYQDWVGPKFTSLEHGADDHHVAAGEPFE"
     gene            4800..5732
                     /locus_tag="VF240_18860"
     CDS             4800..5732
                     /locus_tag="VF240_18860"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177326.1"
                     /translation="MKIGGYKVELVKRLEPSLGATFVRRGDALLVAREAEDGGYELEL
                     FELDGLKSIGRAPVEGVTAVARAGEQAVLLTVRPGRQERSANRRERKLEVRDLKDFRV
                     LTTIVEDKPGYIAASPDGSRIAVAHDFGALNIWDAGTGKLLSEYASKGVGGVAYSHDG
                     ALLAAKEFRGELKIFDATKPGEKPLRSVAAGSGEALIAFHPREHIVAAAGKNAIKIVD
                     ANTAKATASLKTTKKESQGAIGHMAFSPDGKLLVTATLSDGVVGFWDLEEAEFIGHAL
                     ELKEPLSGVEFDAPGKLLLVSSFEAAELYSITKA"
     gene            complement(5751..6206)
                     /locus_tag="VF240_18865"
     CDS             complement(5751..6206)
                     /locus_tag="VF240_18865"
                     /inference="COORDINATES: protein motif:HMM:NF039577.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177327.1"
                     /translation="MLHPFRAGANMDFIIINGDQAIFDASFPPATVIAPPGIITGTAM
                     AKVSGLLACAEGDEASVVVPGAVYFTPSFPIPGVGTLTIESLGPDQKAAKGKSSGRLL
                     ILRGAKFRARFQVAAPAQVPVTGGTVPDPTPVYFGTGSFITTNTRAQAQ"
     gene            6351..7439
                     /locus_tag="VF240_18870"
     CDS             6351..7439
                     /locus_tag="VF240_18870"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7177328.1"
                     /translation="MKPHKSLAAVLLTLAGVALAYLLLWPVPIDPQAWTPPEPPPLAG
                     VYEPNTRLAAVERIGAGVGFAPEGVAIDAEGRIYSGTADGRILRFEADGSQPTVFAQT
                     GGRPLGLEFDRAGTLIVTDANKGLLAVGRDGAVSVLSTQAQEGRPFRCTNDLDVAADG
                     AIYFTDASDKFPLEVYKEDIIEHRPRGRLLIYDPASRTTSVVLDGLYFANGVAVSPDQ
                     TFVLVVETGKYRVRRVWLAGERKGQSDIFIDNLPGFPDNITSNGAGKFWLALITPRSR
                     LLDTLLPYPFLRKVILRLPQSFQPAPERYGFVLALDTEGRVVENLQDPSGKSFAQISS
                     ALEHRGKLYFGSIGEDALGRLLLPSANN"
     gene            7689..8840
                     /locus_tag="VF240_18875"
     CDS             7689..8840
                     /locus_tag="VF240_18875"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX7177329.1"
                     /translation="MINLLSSATFVPPHKFTTPDLVAALGRKLSPEMLTTIGSLGVNQ
                     RYSALENYPNFLTGESMHPTISATDLAVAAGKKCIEQWGGDPRKIGLLVAATNTPAQL
                     LPCLASEVMANMHGVLSRSISTVSMQAQGCSVLLKSIEVAQWYLCSNPHNVALVLMSE
                     AHTPYITQLLREEYFGYREIAQMRKSRSADEAGIARERLNTTLVVQAMLFGDGAVALL
                     IGREDGEATFGPITHLTNDDPEDVKLLTMEGGSLHPCVGGKPQYFMQPKVPGRGAHYA
                     AKTVKRTLEHPQSPVHSLEQVEGYLIHTGSRKILDGVCSQLSLSPESPRVRTSYEVLE
                     SYGNLSSASTGFMLAEKSVRQGVHLAVGFGVGFTASAGLISFHGSGTNH"
     gene            9012..10544
                     /locus_tag="VF240_18880"
     CDS             9012..10544
                     /locus_tag="VF240_18880"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX7177330.1"
                     /translation="MSAIEETKTEIGVQKKAAPRAASASVVTRVLDFLSSVRFGVSLL
                     ILLSVACMIGMLVMQQNVDGFDKYYLDLSPATRLLFGALGFFDIYHVWYFNALLLVLS
                     LNIVLASIDRFPKAWTFVSRPKLDASAHWLRGQEQSATVTLADDGAQGTAAERVASAF
                     SAHGLKPRLTEKGGRTFVFGERAVWNRLGAYSVHVALLTIFTGGFLTSQFGFTGNLSM
                     EPGGSGNKIVERVLDLDEPKLVTTPLPFTVECIDIQQKLIRKDGPITADNTLDWLTTI
                     RIKDPIYGEREGVTHMNTPLDFKRGWWWDNYRFFQASFTPNGKARSITLRVAPEGGGA
                     AQDVTIARDGAGVLTDGTRIEFKDFSAQFSAGQQQAQSEQVVYTNPAATLLVHPAAGG
                     APERAFAFTPQMAENAPIAKRAVGGYTFRLVDFEKVPETHVLSVQRDPGATVVYIGFI
                     LLGLTLCAVFFFSHQRVWALIEERGQGRYEVVLGGNTNRNKLGFEDRFKRIAAALGGP
                     NS"
     gene            10559..12229
                     /gene="ccsA"
                     /locus_tag="VF240_18885"
     CDS             10559..12229
                     /gene="ccsA"
                     /locus_tag="VF240_18885"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7177331.1"
                     /translation="MSEVLKRSAFVAGHVGDAEPKTTALGSYAPAFVAVGGALALALL
                     RYTMGGANFISDGALMMLALAAYLIAAVFCLTNLYAPATLFQRIGLWGATAGVFFNLS
                     SWLVRWFFAYDAETTVLAAQGVPASEWPWLFRYIPFANLYDLSLAFAFGAGITTLVIA
                     HRKNFRFLGAISLPMAALILVLARFIGDDLIQLPPVLDSYWRPIHVGIASLSYGVALV
                     CFAVAVVYLLKDGVKTEAMAIWTSLFALFIILTVSRGTVFTLGSVFWSFDPAAFGTYR
                     ASTVIGNSRSALALRADLPFVGPLLVIAGLMLAGVVAAFLVYLNKQSESARAWGHRLL
                     VAALIVQGLAIVTLAYQVKNTGDVLARVNTSQYERFTVWMIEQEMTQQRAPAQAIKAE
                     VASYTPEQKYQLADRWVRDNGARLRLRLGANPVEFAALITCFAATLFVVIFSFRTEKL
                     RDALPPLPKIDSLMYKTAGVTFAGLALLLMTGAVWANESWGRYWGWDPKEVGAFVAWL
                     TYGGFLHTRISRGWTGRSSAYFALVAFLLIIFTYLGVSYLLPGLHSYA"
     gene            12358..13131
                     /locus_tag="VF240_18890"
     CDS             12358..13131
                     /locus_tag="VF240_18890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177332.1"
                     /translation="MTKENVLYSIIGLLLGYVVAFTFVTYVNQREAGAGATAAAVQGG
                     AEPATGRPAGSAQEEEIRERLRQIDAAAREQPEDFDLQMRAGQAHGAIGEFEEAIDFL
                     SRARELRPDDYDVTVELGNQNFEAGRYEVAERWYKEALAKNPDDINVRTDLGLTFFFR
                     TPRDTESALAEFRRSLERDPRHELTLQNMTAVLASRAALRDADAALRRKSLEEAEQTL
                     VRLEALNPSNPSLPKLRDSLREARESLGAQPVSAAKERG"
     gene            13314..13844
                     /locus_tag="VF240_18895"
     CDS             13314..13844
                     /locus_tag="VF240_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177333.1"
                     /translation="MRHFFIRVLLCAAVAQVACGGGNAEKTAETNRPATATTLAHGPS
                     ANVPPASTTPPAAAPPVASSHGASAPSVPSAGQGRASADTAALDAKIAGALAKAKAPK
                     AGAAEKKAAALAYLERGNVYWSAGDPTLYKFALADFKSVLVYDPANAEAASKRDEIVR
                     IYRAMNRPVPELSNEK"
     gene            14140..14829
                     /locus_tag="VF240_18900"
     CDS             14140..14829
                     /locus_tag="VF240_18900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7177334.1"
                     /translation="MSALSPVSELFLSVPVAERQPAPAAAASAAAAAPLSEPLSDHGL
                     LEATLAGDEAAFAELVRRYRNQITNYAYRITNDYDAAVDLAQETFLRVFRAADRYQTT
                     YAFSTYIYRIATNLAISELRRRKRRRLVSLATFFQGRDGAPGEATEFDPPDACPLQDA
                     ALVDDERREAVARAISTLPEKYRAPLVLRDVEGRSYEEIAGILEMSEGTVKSRINRAR
                     TFLREKLQAYI"
     gene            14851..15678
                     /locus_tag="VF240_18905"
     CDS             14851..15678
                     /locus_tag="VF240_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177335.1"
                     /translation="MNCKAYRDEADAADLGDAPGAAARAHLALCDACRIFRDEREGLR
                     RLLGGMGSVAAPADFEFRLRAAMRAGGGGPRPGLHARLFKPLSAALAACAAVAFTVAY
                     LRAPADAPRQDLAAVTSSEAHAPAASQTQTPDAPATGTSASSIRDSAASDVAVVSSVG
                     PKRDAQTRRATHRTVLNQSRGVKGFEEAARVTADSALRAAPVRTRLGSGGAAAGSNTP
                     VPVQLSASRKPMRVVLRDERGASRVVSMRPVSFGSQEFVGRLNNSYRAPNSTKEGVW"
     gene            15694..17475
                     /locus_tag="VF240_18910"
     CDS             15694..17475
                     /locus_tag="VF240_18910"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7177336.1"
                     /translation="MKCSVINRGARARPWGAACLCALALLFVLPATGHAQHQQGRGEV
                     AQTPPAKAAPRAVESEQRPEAKGVPAQAPAPVVVPGAPVRMPGAVLPAAPAPPTAQTP
                     RAAAVPQAAPRNVVTVVHRLNGWQMLSLMAAKGGERWVIDELPPAAEFHTNIVAGFVS
                     EDGRTVMARLPTAEAELFAAAASMFPALPGAPAPPPDRLEGAPGFTVIRSDGKRVGAR
                     FVGLDASTGLSLLEAAEQLLVSAPHAPVVAPAVGQRVRLFAPAPALAPFAPAAPPVSA
                     APPHPAGDSGHTETIYFSIGETKGQLTEVRRASTGKPVQATVRSKLLTPEWAGAIATS
                     EEGDFVGIVGDSGHTESAIVPAEAIRLARERVLARRASVPRPWLGVSGDAVSRFSLEQ
                     MLARGWPRDFALPLLNRPQGVLLTQITPGAPAALAGLRPGDIISRVGQREVSGVDDFS
                     MLLREAGAGARLDLTVLRALERAPLNLTVELSGTLLPGVRPDDVQNPLVGHGAETVGL
                     SPRGAAPLRARGGLLVVSVHAGTNAARAGLRPGDIVETINGLPVTRAGVRPLPGVPPS
                     DMTLGVVRDGRRLDLRLAHAEPPPEKP"
BASE COUNT         3202 a         5860 c         5639 g         2837 t
ORIGIN      
        1 aggagcagat gcagcagcgg gccaaacgcc cgcgacggcc cgaaatcact ccacagttcg
       61 agaccgacct ccgactgctg cgccgcgccg gcgggcagcc tttgcccggc cacctgcgcg
      121 ccttccttga gccgcgcttc gggcacaatc tcggcgacgt gcgcgtccac gtcgatcact
      181 cagccgccga gctggcccag aaggccaacg cgcgggcctt caccgttggc aagcacatcg
      241 tcttcgcggc gggcgagtac cgacccggcg tcgaacaggg ccggcggctc atcgcgcacg
      301 agctgacgca cgtcttccag caacgcggcg ggctgcacag cgtgcagcgc gaagtcgggc
      361 ccgaacgcgt cgccaccgcc gatccgcagg ccgatctgcc gagcctcgaa gagttgcgcg
      421 ccgccttcaa actcgacgcg gcgtccgcgc cgccctcgat tttgagcgtg gccgtcgccc
      481 tgctccgcaa cgcgctgcgc agcacgtcca acgcccagcg gctccgaccg ctcacgcgaa
      541 gcgaaggcga aacagcagcc ctcgtccgcg ccatccagtc gggcgcatac acgctcgaac
      601 tcgcgtccct gcgcggcggc cgggagaagg gctggaagct tactcaacgc accagacgtg
      661 agacgttcgt ctcgcgctcg aactcagcca cacaacagtg gcctgcggcg acggccggcg
      721 acgacacgat cacgctgccc accccgcccg ccgccgaaga ctatccgcaa ggcaccatcg
      781 tcgccggcct cgaatccatc tcgccgggcg accgtgtgga aggcgcaaag cctactccac
      841 tgattccgaa aaccgtctca gaaggcgccg tacccgtcag gaagtcttcg cttcccacac
      901 ccgtccccga aactcaaacc gtccccacgg ccgcgcccgc cgctaaagtt gttgccgaaa
      961 gcgtcgaagc tcccgccgag tctcttaaag aagctgcgcc ggcagcggac gcggccacgg
     1021 cggccgcaca gccggcgact tccgaagccc ccgcggcaga ggacggcgcg cccgcggcgg
     1081 cggagcctga gcaggccccg ctcgacccgc agaaagaccc ggagtttcag cagaccctcg
     1141 gccagatcaa acgcacgcgg aaagctcagg gcgcgcacat cccttccgac gacaagctcc
     1201 agcataccaa ggacgccacc gtcctcaagg aggaaaaaca gcgagagaag cacgaccgcg
     1261 acaagcacct cacggagatc gacgccaccg ccaagaagac cgaaagcgtc cccttcacgc
     1321 ccgaggagtt caagaagctg ctcaatgcga gcatcaagaa aatcgagacc aagctcccca
     1381 ggaatgaaga cgatgccaac cgcttcaaaa aggagaggcc cctcgaacag acgaaggagg
     1441 aagtccgcgg tcaggtcaag aagaagaacg acgaaatcgc cggcccgctg gcgacacagg
     1501 tcgatcccga aaagccgccg cctcaggcca aaccatttga cccgaagccc accgccgatc
     1561 tgatagagga agaggtgggc aagaggccgc acaacatcag tccggccgcc gccgccccca
     1621 agccccgctt cgactccgaa atctcgatgg agaaagagag ccagtcgctc gacgacttga
     1681 tgtcggagaa cgagattacg gaggagcagt tggccgagtc gaacgagccg acgttcgtcg
     1741 aggcgttagg ctcgaagaag gccgcgcagg agaaggccgc gcaggcgccc gcgcagttcc
     1801 gcgccgtgga gcagcccatt ctggataagt cgcaggcgaa ggccgggcaa aagggggcgt
     1861 caaagttcgg ggcgatgttc aagggccgcg acgaagcctt cggcctcgtc tccggcaaac
     1921 aaaaattgac cgtcaatgac gataaggcga agcaggaaga cttcctcatc aagctgaaag
     1981 ggatctacga gagcaccaag accgatgtcg agggcatcct cgatgctctc tccaacaccg
     2041 tggacgccct cttcaccctc caggtgacac tcgccaaaaa cctctttgag tcgcaggtgg
     2101 agtcgcagct cgacgacatc tacggcttca ccacctggga cgactcgctt ttcggggcgg
     2161 acaccgaggc catcgaaggc gttttccggc gggagaagga tcgcttcctg gcgacgatgg
     2221 acaggatcat tgaccagatc gccaagttca tcgccgacat gttgaacgcc gcgatggcgc
     2281 gcatcaagag gggccggaag gaggccgacg atctcttcgc gtccctcagc acagagcagc
     2341 agcggctctc gacggatgcg atggggttgt tcaagacgca gtttgaaatg ctcgaagacg
     2401 gcgtccgtga gaagcagcag gatttggccg acacgctggc cgagtcttac aagagcaacg
     2461 tcgattcgct ccgcgagagc ttcgacaaga tcaaggaaga catcagcacg ggctggatcg
     2521 gcaaggcggt caatttcatc aaaagcgtcg ccgagaccat caagaagctg ggggagctgc
     2581 tcttgtcgat cctgtcgcgc atcggcgcga tcatcggcga catcctcgcc caccccatcc
     2641 gcttcatcga gaatctcgcc aagggcgtgg gcgacggctt cgacatgttt atcgagaggc
     2701 tcgacgacta cctcatcgcc ggcttcttcg actggatacg gggcacggtc ggcggccccg
     2761 gcatcgacat ccccgacaag ttcgacgcgg cgggcatctt cagcctcgtg gcgcaggtcg
     2821 cgggcctctc ctacgacacc ttccgcaagg tggcgtcgaa ggtctggggc aaggccgcgg
     2881 tcgagttcat cgatcagggc aaagccgtcg ccgaaaaggg tctggagatt ttcttcatcg
     2941 tcaaggagaa gggactcggc gggctgtggg agcacatcca ggaacacctc ggcagcatgg
     3001 ttgacgacct gatgacgaag gtcaaggaga ccgtcctcta cgaagtcatc aagaaggcgc
     3061 tcgcctacat cgccggcctc ttcaaccccg tcggcgcgtt catcaaggcg gcgcaggcca
     3121 tctacgccgg catccgcttc ctgatggaca acatccagcg gatcgcggac atcgttgacg
     3181 ccttcctcga ctcgctggag ctggccgtgg ccggcaagac cgacctcatc tcgcagaaaa
     3241 tcgtcacggc gctacgcggc gtcatcgtca tcgccatcga cttcctcgcc aagctcctcg
     3301 ggctgggcga cctcgacgag aaggtgcgta aaatcctgaa agtcatccgc acgcccgtcg
     3361 agcgcgcgat ggaagtggtg ctcaaggcgc tgcggcctct cgtccagaag gtgatgagaa
     3421 cgctcggcgt cggcaaggac gaggaggagg cggagaagga agtcgagaag aagggcggcg
     3481 agccgcaaac gcacgacgag gtgctcgacc acgtcgtcgg gctgatggcg caggagacca
     3541 aggcggacac gcccgccctc gcgctcgaag agaagaagga gcaggcgcag aacctgatca
     3601 agaagtacca gcccctgctc aagcacggcc agcttcaaat cctcatcaat gacaagaaca
     3661 gcgaagacgt ggtggaggat tcggcggtgg acttcgatgt gtcggcaagc cccgggcgga
     3721 aggggcaggc gctggtgccg atcaaagtcg tggacgagag ggcggtgcag gagcgcttct
     3781 cgcacgcgcg gagcctcaag aagttcacgg acgagaaagg gttcgggctg gacgactggg
     3841 ccggcacctt ctcgcacctg agccacagca cacatctggc tgacatgcgc tacggcttgg
     3901 atagcaaagt gaagctactc acgaagcaag gggacacgta tcacttcaag gccgacgtca
     3961 aggatgcaga ggtggtgaga ctcggcgcgc tcgaactcca gaacgaaggc cgccaccacg
     4021 cgccgttaca gaagggccgc ttcggcgtgc cgctcatcca gaccttcctg tccgggagaa
     4081 agatcgccgg catcccgccg aacatcttcg acgaccgcgc ggtcgtctcg aaggtcgcgg
     4141 cggcggccga gggcgccggc gccatcgtca aggtcggcaa gaccgatacg tgggcgctcc
     4201 ccggcataca gcctcaacgc ctcctacccg acaactggcg cggctcagac aacatccgcc
     4261 cgcgcttcta ctataaagag gcaggattcg ctgatctggc cgacgagttg tttgagaagg
     4321 tgatcaaaga tgaaatcgtt gacctcatcg aagcgcaact ggaaaacata gaagatgcca
     4381 ggaagctggg cctgccgccg tcgcaagccg acatgaagcc ctgggagacg atgaaaaaaa
     4441 gagaatacgc ggacacgaat gagaagttca gcgtcgggct tgcgaggata gacggctact
     4501 accagcgcaa ccgctacgat gtggatcacg tgacttcgct cgccaagcac tggaacaaca
     4561 agggccacga cgacggccag accgaacgca tcgcggcgac gcagggcaag cgcggcggcc
     4621 tcgccctgat ggagaagggt ctgaaccgca gcaaggggtc gggcggcatt aattatcagg
     4681 attgggtggg gccgaagttc acctcacttg aacacggggc ggacgaccac catgtcgccg
     4741 cgggcgagcc gttcgagtga gtcccgacga gcaggccgca gacaaaaggg tcaagacgaa
     4801 tgaaaatagg cgggtacaaa gtcgagctgg tcaaacggct ggagccgtcg ttgggcgcga
     4861 cgttcgtccg tcgaggagat gcactgctcg tcgcccggga agcggaagac ggcggctatg
     4921 agctggagtt gtttgaactc gacgggttga aatcaatagg gagggcgccg gtcgagggtg
     4981 tgaccgccgt cgcgcgagcc ggggagcagg ccgtcttgtt gaccgtgcgg cccgggcgtc
     5041 aggagaggag cgcgaacagg cgcgaacgga aattagaagt ccgcgacttg aaagacttcc
     5101 gcgtgctgac gaccatcgtg gaggacaagc ccggttacat tgccgcgagt cccgatgggt
     5161 cgcggatcgc cgtcgcccac gacttcggcg ccctgaacat ctgggacgcc gggacgggga
     5221 aacttctttc cgagtacgct tccaaaggcg tcggcggagt cgcctacagc cacgacggcg
     5281 cgttgctcgc cgccaaagag ttcagagggg aactgaaaat cttcgacgcg acaaagccgg
     5341 gagagaagcc cctgcgctcc gtggccgccg gcagcggaga ggcgctgatc gcttttcacc
     5401 cgcgcgagca tatcgtcgcg gcggctggta agaacgccat caagatcgtg gacgccaaca
     5461 cggcgaaggc gacggcgtcc ctcaagacga cgaagaagga gagtcagggc gccatcgggc
     5521 acatggcttt ctcacccgac gggaagctgc tcgtgacagc gacgctcagc gacggcgtcg
     5581 tcggattctg ggatttggag gaggccgagt tcatcggcca tgcgctggag ttgaaagagc
     5641 cgctgagcgg cgtcgagttc gatgcgccgg gcaaactcct gttggtctcc tccttcgagg
     5701 cggcggagct ttactcgatt acgaaggcgt aggtggccgg cgtgggcgcg tcattgcgcc
     5761 tgcgcgcgcg tgttggtggt gatgaagctg ccggtgccga agtagacggg cgtcgggtcg
     5821 ggcacggtgc cgccggtgac ggggacttgc gccggggcgg ccacctggaa gcgcgcacgg
     5881 aacttggcgc cgcgcagaat caggaggcgg cccgacgact tgcccttcgc ggccttctga
     5941 tccggcccca gactttcgat ggtgagggtg cccacgccgg ggatcggaaa cgaaggtgtg
     6001 aagtagacgg cccccggcac caccacggac gcctcgtcgc cctcggcgca ggcgaggaga
     6061 ccgctcacct tcgccatcgc ggtgccggtg atgatgcccg gcggggcgat gaccgtggcg
     6121 ggcgggaaag aggcgtcgaa gattgcctga tcgccgttga tgatgatgaa gtccatgttg
     6181 gcgccagccc gaaacgggtg cagcatagca gaaggccgcg cggatgtgtt gcaaagaata
     6241 agttgatgca cggaaagtcg tcgaatcgca attcaacttg tattgcgtga cggaaaaaat
     6301 gttactctcg gcgcgccccg gcaacttcgg aattcgcgga tgcacacagc atgaaacctc
     6361 acaagtctct ggccgccgtc ctcctgactc tggcaggcgt ggcgctggcc tatctcctgc
     6421 tctggcccgt ccccattgac ccgcaggcgt ggacaccgcc ggagccgccg ccgctcgccg
     6481 gtgtctacga gccgaacacg cggctggcgg ccgtggagcg catcggcgca ggcgtgggct
     6541 tcgcccctga aggcgtcgcg attgacgcgg aagggcgaat ttattccggc acagcagacg
     6601 ggcgcatcct gcggtttgag gcggacggca gtcagccgac ggtcttcgca cagacggggg
     6661 gccgtccgct ggggctggag tttgaccgcg ccggaaccct catcgtcacc gacgccaata
     6721 agggactgct cgccgtcgga cgcgacggcg ccgtgtccgt cctgagcacg caggcgcagg
     6781 agggccgccc cttccgctgc acgaacgacc tcgacgtggc cgccgacggc gccatctact
     6841 tcaccgacgc ctccgacaag ttcccgctcg aagtctacaa agaagacatc atcgagcacc
     6901 gcccgcgtgg ccgtctgctg atctacgacc ccgcgagccg gacgacgagc gtcgtgctcg
     6961 acggcctcta cttcgccaac ggggtcgcgg tcagccccga tcaaactttc gtgctggtgg
     7021 tggagaccgg aaagtacagg gtgcgccgcg tgtggctcgc gggtgagcgc aaagggcagt
     7081 ccgatatttt catcgacaac ctgccggggt tccccgacaa catcacttcc aacggggccg
     7141 gcaaattctg gctggcgctg attacgccgc gcagtcggct cctcgacacg ctgttgccct
     7201 acccgttcct gagaaaagtc atcctgcgtc tgccccaatc ttttcagccc gcccccgagc
     7261 gttacggctt cgtcctcgcg ctcgacaccg aggggcgcgt ggtcgaaaac ctgcaagacc
     7321 cctcgggcaa gagcttcgca cagatctcca gcgccctcga acaccgtggg aaactttact
     7381 tcggcagcat cggtgaggac gccctcggcc gtttgctatt gccgtcggcg aacaattgat
     7441 ttaatatccc gccattgtga atgcgacgtt agagtgtcgg gcggtgttcg cttacccgat
     7501 gacacgccga agggtctaac gcctaccggt tagttgatcc atacactagg aagacattca
     7561 tagttgaaag cgtagtcggt ctttgccggt ctgccgctgt tactatggcg gcagcccatc
     7621 gcgccgcaag cccccgtgcg cggcgcgttt acactcagcc ccaacactcc aggaaagagg
     7681 ccctcacaat gattaatcta ctctcctcgg cgacctttgt gcccccgcac aagttcacca
     7741 cccccgatct cgtcgccgcg ctcgggcgca agctctcgcc agaaatgctc accaccatcg
     7801 gcagtctggg ggtaaatcaa agatactccg cgctggagaa ctatcccaac ttcctgaccg
     7861 gggagtcgat gcacccgacg atttcggcga ccgacctcgc cgtcgcggcc gggaaaaagt
     7921 gtatcgagca gtggggcggc gacccgcgca agatcggcct gttagtcgcg gcgacgaaca
     7981 ctcccgcaca gcttctcccc tgcctcgcct ccgaggtgat ggcgaacatg cacggcgtcc
     8041 tttcgcgctc gatcagcacc gtgagcatgc aggcgcaagg gtgctccgtt ctcctcaagt
     8101 cgattgaggt ggcgcagtgg tatctctgct ccaaccccca caacgtcgcg ctcgtcctga
     8161 tgtcggaagc ccacacgccc tacatcacgc agctcctgcg tgaagaatat ttcggctatc
     8221 gcgaaatcgc ccagatgcgt aagagccgca gcgccgacga agccggcatc gccagggagc
     8281 gtttgaacac cacgctggtc gtgcaggcga tgctcttcgg cgacggggcc gtggccctgc
     8341 tcatcgggcg cgaagacggg gaggcgacct tcggccccat cacacatctg acgaacgacg
     8401 accccgaaga tgtaaagctg ttgacgatgg aaggcggaag cctccacccc tgcgtgggcg
     8461 gcaagccgca gtatttcatg cagcctaaag tgccgggccg gggcgcgcac tacgccgcta
     8521 agacggtgaa gcggacgctt gagcatcccc agtcgccagt ccatagtctc gaacaggtgg
     8581 agggttacct gatccacacc gggagcagaa aaattctcga cggcgtctgc tcgcagctct
     8641 cactgtcgcc ggaatcgccc agggttcgca cctcctacga agttcttgag agctacggca
     8701 acctttcgag cgcctccacc gggttcatgc tcgcggagaa aagtgtccga cagggggtac
     8761 acctggccgt cggcttcggc gtcggcttca cggcgagcgc cgggctgatc agctttcacg
     8821 gctccggcac gaatcattag gaaggcaaaa ggcaaaagta aaaaggcaaa agtgaggaag
     8881 cgtctcacct cttcactttt gccttttgcc tttttacttt tgccttcttc agtgaagttg
     8941 ccggcctgtg ggccttcggt tatcattacg cacactcgat ctgaagtttt gaagtgccag
     9001 cccgctaaaa catgtcggca atagaagaga ccaagaccga gattggcgta cagaagaagg
     9061 ccgcgccgcg tgcggcttcg gcctccgtcg tcacacgcgt cctcgacttc ctcagctccg
     9121 tgcgcttcgg cgtctccctg ctcatcctgc tctccgtcgc ctgcatgatc ggcatgctcg
     9181 tcatgcagca gaacgtggac gggttcgaca agtattacct cgacctgtcg ccggccacgc
     9241 gcctgctctt cggcgcgctc ggcttcttcg acatctatca cgtctggtac ttcaacgcgc
     9301 tcctcctcgt cctctctctc aacatcgtgc tggcctccat cgaccgcttc ccgaaggcgt
     9361 ggacgttcgt ctcgcgcccg aagctcgacg cctcggccca ctggctccgc gggcaggagc
     9421 agagcgcgac cgtcacgctc gccgacgacg gcgcgcaggg cacggccgcg gagcgcgtcg
     9481 cgtcggcctt ctcagcacac ggcctcaagc cgcgcctgac cgagaagggc gggcgcacct
     9541 tcgtctttgg cgagcgcgcc gtgtggaacc gtctgggcgc ttactcagtc cacgtcgccc
     9601 tgctaacgat tttcacgggt ggattcctga cctctcagtt cggcttcacc ggcaacctgt
     9661 cgatggagcc gggagggtcg ggcaacaaga tcgtcgagcg ggtgctcgat ctcgacgagc
     9721 cgaagctcgt caccacgccg ctgccgttca cggtcgaatg tattgacatc cagcagaagc
     9781 tcatccgcaa ggacggcccc atcacggccg acaacacgct cgactggctg acgaccatcc
     9841 gtatcaaaga ccccatctac ggcgagcgcg agggggtcac ccacatgaac acgccgctcg
     9901 acttcaagcg cggctggtgg tgggacaact accgcttctt tcaagccagc ttcacgccga
     9961 acggcaaggc gcgctccatc acgctgcgcg tcgcgcccga aggcggcggc gccgcgcagg
    10021 acgtgaccat cgcgcgcgac ggcgcgggcg tgcttaccga cggcacgcgc atcgagttca
    10081 aagatttctc cgcgcagttc agcgccggcc agcagcaagc gcagagtgag caggtcgtct
    10141 acaccaaccc ggcggcgacg ctcctcgttc acccggccgc gggcggcgct cccgagcgcg
    10201 ccttcgcctt cacgccgcag atggccgaga acgcgcccat cgccaagcgg gcggtcggcg
    10261 gctacacgtt ccggctcgtc gatttcgaga aggtgccgga gacgcacgtc ctctccgttc
    10321 agcgcgaccc cggcgcgacg gtcgtctaca tcggtttcat cctgttgggg ctgacgctct
    10381 gcgccgtctt cttcttctcg caccagaggg tgtgggcgct catcgaggag cgcggccagg
    10441 ggcgctatga ggtggtgttg ggcggcaata cgaaccgcaa caagctcggc ttcgaggatc
    10501 gcttcaagcg aatcgccgcc gcgctcggcg gaccgaacag ttgaggtgaa gtaagattat
    10561 gagtgaagtc ttaaagcggt cggccttcgt cgccggccat gtcggtgacg cggagccgaa
    10621 gaccacggcg ctcggttcat acgccccggc gtttgtggcc gtcggcggcg cgctcgcgct
    10681 ggcgctgctg cgctacacga tgggcggcgc aaacttcatc tccgacggcg cgctgatgat
    10741 gctcgcgctc gccgcctacc tgatcgccgc cgttttctgt ctgacgaacc tctacgcccc
    10801 ggccacgctc ttccagcgca tcgggctgtg gggcgcgacc gccggcgtct tcttcaatct
    10861 gtcgagctgg ctcgtgcgct ggttcttcgc ctacgacgcg gagacgaccg tgctcgcggc
    10921 gcagggcgtc ccggcgagcg agtggccgtg gctgtttcgc tacatcccgt tcgcgaacct
    10981 ctacgacttg agtctggcct tcgccttcgg cgctggcatc acgacgctcg tcatcgcgca
    11041 ccgcaaaaac ttccgcttcc tcggcgcgat ctcgctgccg atggccgcgc tcatcctcgt
    11101 gctcgctcgc ttcatcggcg acgacctgat tcagctcccg cccgtgctcg actcctactg
    11161 gcggcccatc cacgtcggca tcgcctcgct ctcctacggc gtcgcgctcg tctgcttcgc
    11221 cgtcgcggtc gtttatcttt tgaaggacgg cgtgaagacg gaggcgatgg ccatctggac
    11281 gagtttgttt gcgctcttca tcatcctgac cgtcagccgc ggcacggtct tcacgctcgg
    11341 cagcgtcttc tggagcttcg acccggccgc cttcggcacc taccgcgcct cgaccgtcat
    11401 cggcaactcg cgctcggcgc tggcgctgcg cgcagacctc cccttcgtcg gcccgctcct
    11461 cgtcatcgcc ggcctgatgc tcgccggcgt cgtcgccgcg tttctggtct acctgaacaa
    11521 gcagagcgag tcggcgcgcg catgggggca ccgtctgctc gtcgcggctt tgatcgttca
    11581 gggtctcgcc atcgtgacgc tggcctatca ggtgaagaac accggggacg tgctcgcgcg
    11641 cgtcaacacg tcgcagtacg agcggttcac cgtctggatg atcgagcagg agatgacgca
    11701 gcagcgcgcg ccggcacagg ccatcaaggc ggaggtggcg agctacacgc cggagcaaaa
    11761 gtatcaactc gccgaccgct gggtgcgcga caacggcgcg cgtctgcgtc tgcgcctcgg
    11821 ggccaacccc gtggagtttg ccgcgctgat tacgtgcttc gccgccacgc tcttcgtcgt
    11881 catcttcagc ttccgcacgg agaagttgcg cgacgccctg ccgccgctcc cgaagatcga
    11941 ctcgctgatg tacaagacgg cgggcgtcac cttcgcagga ctggcgctgc tgttgatgac
    12001 gggggcggtg tgggccaacg agtcgtgggg ccgctactgg ggctgggacc cgaaggaggt
    12061 cggggccttc gtcgcgtggc tcacctacgg cggcttcctc cacacgcgca tctcgcgcgg
    12121 ctggacgggc aggtcttcgg cctacttcgc cctcgtcgcc ttcctcctca tcattttcac
    12181 ctacctcggc gtcagctacc tgctgccggg cctgcactct tacgcctgac ggcgcgtgat
    12241 aagtgatgag tgataagtga taagaaagaa cggaagctgt tagctcttag ctgttggctg
    12301 ttagctaatc gctaagggct aacagcttct tatcactcat cacttatcac gtcatttatg
    12361 acaaaagaga acgtcctcta ctcgatcatc gggctgctgc tgggctacgt cgtggccttc
    12421 acctttgtca cctacgtcaa ccaacgcgag gcgggcgcgg gggcgacagc ggcggcggtg
    12481 cagggaggcg cggagcctgc tacggggcgg ccggcaggtt cggcgcagga ggaggagatt
    12541 agggagcgcc tgcgccagat tgacgccgcg gcgcgcgagc agcccgaaga cttcgacctc
    12601 cagatgcgcg ccgggcaggc gcacggcgca atcggggagt ttgaggaggc gattgacttc
    12661 ctgtcgcggg cgagggagtt gcggcccgac gattacgacg tgacggtcga actcggaaat
    12721 cagaacttcg aggccggtcg ctacgaggtt gccgagcgct ggtacaagga ggcgcttgcg
    12781 aagaacccgg acgacatcaa cgtccgcaca gacctcggcc tcaccttctt cttccgcacg
    12841 ccgcgcgaca cggaaagcgc gcttgccgag tttcgccgct cgctggagcg cgacccccgc
    12901 cacgagttga cgctccagaa catgacggcc gtgctcgcca gccgcgccgc gctccgcgac
    12961 gccgacgcgg cgctgcgccg caagagtctc gaagaggccg agcagacgct cgtccggctc
    13021 gaagccctca acccctccaa cccgtcgctc cccaaactgc gcgactcgct cagggaggcg
    13081 cgcgaatcgc tcggcgccca acccgtctct gccgccaagg agcgcggctg aaatctcccg
    13141 cgcctccagc gtgagtacat cggcgcttga gaacagtatc gcgagcggaa gcctcgcggg
    13201 tctgccgcgc gcgaggggga ctctggtcga tagcacaccc gctcgctacc gctcgcggta
    13261 ctgtgattcg ggcaactctc acgctggagg cgcaaacagg agcatctgaa acaatgcgtc
    13321 atttcttcat tcgtgttttg ctgtgtgcag ccgtggcgca ggtcgcgtgc ggcggcggca
    13381 acgccgaaaa gacagccgag acgaacaggc ccgcgacggc gacgaccctc gcgcacggcc
    13441 cgtcggccaa cgtcccgccg gcctccacaa cgccgcccgc cgccgcgccc ccggtcgcgt
    13501 cctcgcacgg cgcgagcgcg ccaagcgtcc cgtcggcggg gcagggcaga gcgtctgccg
    13561 atacggcggc cctcgacgcg aaaatcgccg gcgcgctcgc gaaagcgaag gctccgaagg
    13621 ccggagcggc agagaagaag gcggcggcct tggcctatct cgaacgcggg aacgtctact
    13681 ggagcgccgg cgaccccacg ctctacaagt tcgccctcgc cgacttcaag agcgtgctcg
    13741 tctacgaccc cgccaacgcc gaggccgcct cgaagcgcga cgagatcgtc cgcatctacc
    13801 gcgccatgaa ccggcccgtg cccgagctga gcaacgagaa gtgaagagct attagcgatt
    13861 agctgttagc tattagccag agccgtccgg cgcgtcgccg ccccatcccg tcaaatcgcg
    13921 cgttcgccat attccggctg atagctaaca gctaatcgct tcattatttt ggaaccaaca
    13981 ggtctgctcc ggcgtgtgtg cctgccggct gggacggccc gtgcggtgta taatgccggc
    14041 gtgtcggccc ggctgtccga ggaggacggc ccgcgttgag cggcccatga ggccgagttg
    14101 gccgcgttcg gattcgggtt tcggagcctg cctaccctga tgagcgcact ttcccctgtg
    14161 agcgagctgt ttctgtccgt gcccgtggcg gagcggcagc ccgcgcccgc cgccgccgca
    14221 tcggccgccg ccgcggcgcc gctctccgag ccgctctcgg atcacggcct ccttgaggcg
    14281 acgctcgcgg gcgacgaggc ggcgttcgcc gagttggtga gacgttaccg gaaccagatt
    14341 acgaactacg cctaccgaat aacgaacgac tacgacgcgg cggtcgatct ggcgcaggag
    14401 acgttcctgc gcgtcttccg cgcggccgac cgctaccaga cgacctacgc cttctcgact
    14461 tacatctacc gcatcgccac caacctcgcc atcagcgagc tgcggcggcg caagcgtcgc
    14521 cgcctcgtct cgctcgccac cttctttcag ggccgcgacg gcgcgcccgg cgaggcgacc
    14581 gagttcgacc cgcccgacgc ctgcccgttg caggacgccg cgctcgtcga tgacgaaaga
    14641 cgcgaggccg tggcgcgggc catctcgacg ctgccggaga agtatcgcgc cccgctcgtc
    14701 ctgcgcgacg tggagggccg cagctacgag gagatcgccg gcatccttga gatgagcgag
    14761 gggacggtca agtcgcgcat caaccgcgcg cggacattcc tgcgcgagaa gttgcaggcg
    14821 tacatttaaa ggaagttggg ttttgaggcc atgaactgca aggcttacag agacgaagcg
    14881 gacgcggccg acctcggcga cgcgccgggc gccgccgccc gcgctcacct cgcgctgtgc
    14941 gacgcctgtc gcatcttccg cgacgagcgc gaaggactcc gccgcctgtt ggggggcatg
    15001 ggcagcgtcg cggctccggc cgacttcgag ttccgtctgc gcgcggccat gcgcgccggg
    15061 ggcggagggc cgcgccccgg tcttcacgcg cgtctcttca agccgctctc cgccgcgctc
    15121 gcggcctgcg ccgccgtcgc gttcaccgtc gcttacctgc gcgccccggc cgatgcgccg
    15181 cgacaagacc tcgcggccgt cacgtcttcg gaggcacacg cgcccgccgc gtcgcagacg
    15241 cagacgccgg acgcaccggc gacgggaaca tccgcctcgt cgatacgtga tagcgcggcg
    15301 agtgacgtgg cggttgtgtc gagtgtcggc ccgaagaggg acgcacagac gaggcgggcc
    15361 acgcatcgaa ctgtgttgaa tcagtcgcgg ggcgtgaagg gctttgaaga ggcggcccgc
    15421 gtgacggccg actcggctct gagggccgcg ccggtcagga cacgtctcgg ttccgggggc
    15481 gcggcggcgg ggtcaaacac gcccgtgccc gtgcagctct ccgcctcgcg caagcccatg
    15541 cgtgtggtgt tgcgcgacga gcggggcgcg tcgcgcgtcg tctcgatgcg gcccgtcagc
    15601 ttcgggtcgc aggagttcgt cgggcgcttg aacaactcct accgcgcgcc gaactcgacg
    15661 aaggaaggcg tctggtaagt gtcgaatagt gttgtgaaat gttccgtcat caatcggggc
    15721 gcacgcgcgc ggccgtgggg ggccgcctgc ctttgcgcgc tcgcgcttct cttcgtcttg
    15781 ccggcgacgg ggcacgcgca gcatcagcag ggacgaggag aagtggcgca gacgccgccg
    15841 gcgaaggcag ccccgcgcgc ggttgagtca gaacaacgtc ccgaggcgaa gggcgtaccc
    15901 gcgcaggccc cggcccccgt tgtcgtgccg ggcgcgccgg tgagaatgcc gggagcggtc
    15961 ttgccggccg cgcccgcgcc gcccacagcg cagacaccgc gcgccgcggc cgttccgcag
    16021 gcggcgccgc gaaacgtggt gacggtcgtt caccgtctga acggctggca gatgctttcg
    16081 ctgatggcgg cgaaaggcgg cgagcgttgg gtaatcgacg agctgccgcc ggccgccgag
    16141 ttccacacca acatcgtcgc gggcttcgtc tccgaggacg gccggacggt gatggcgcgt
    16201 ttgccgacgg cggaggcaga gttgttcgcc gccgcggcgt cgatgttccc ggcgctgccc
    16261 ggagcccctg cgccgccccc cgaccgtctc gaaggcgccc ccggcttcac cgtgattcgc
    16321 tccgacggca aacgcgtcgg cgcaaggttc gtcggcctcg acgccagcac gggcctgtcg
    16381 cttttggagg ccgccgagca gctgttagtg tccgcgccgc acgcgccggt cgtggcgccc
    16441 gccgtcggtc agcgcgtgcg tctgttcgcg cccgccccgg ccctcgcgcc gtttgccccg
    16501 gcggcgccgc ccgtctccgc cgcgccgccg caccccgctg gcgactcggg tcacacggag
    16561 accatctact tcagcatcgg cgagacgaag gggcagttga ccgaagtcag acgcgcctcg
    16621 acgggtaagc ccgtgcaggc caccgtgcgc tcgaagctgc ttactccgga gtgggcgggc
    16681 gcgattgcga cgagcgagga gggcgatttc gtcggcatcg tcggcgacag cggtcacacg
    16741 gagtcggcca tcgtcccggc cgaggccatc cgcctcgcac gcgagcgcgt cctcgcacgg
    16801 cgcgcgagtg ttccccgccc gtggctcggc gtcagcggcg acgccgtctc tcgtttctcg
    16861 ctcgaacaga tgctcgcccg cggctggccg cgcgacttcg cgctcccgct cctcaatcgc
    16921 ccgcagggag tgctgctcac gcagatcacg ccgggcgcac cggccgcgct cgccggcctg
    16981 cgacccggcg acatcatctc gcgcgtcggc cagcgtgagg tgagcggcgt ggacgacttc
    17041 tcgatgctgc tcagggaggc cggggccggc gcacggctcg acctgacggt gctgcgcgca
    17101 ctggaacgcg cgccgctcaa cctcacggtc gagttgagcg ggacgcttct gcccggcgtg
    17161 cgccccgacg atgtgcagaa cccgctcgtc ggccacggcg ccgagaccgt cggactctcg
    17221 ccgcgcggcg ccgctcccct cagagcgagg ggcggcctgc tcgtcgtctc cgtacacgcc
    17281 ggcaccaacg cggcgagggc tggtctgcgt cccggcgaca tcgtcgagac gatcaacggc
    17341 ctgcccgtga cgcgcgcggg cgtgaggccc ctgccgggcg tgcccccctc cgatatgacg
    17401 ctcggcgtcg tccgcgacgg gcgccggctc gacctgcggc tcgcgcacgc cgaacctccg
    17461 cccgaaaaac cctagagcga tttacaaacg cacttcccgt ttagaggcgg gcagtgcccg
    17521 ccgtctcaag ctccgata
//
LOCUS       DASVES010000455         8974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_644146, whole genome shotgun sequence.
ACCESSION   DASVES010000455 DASVES010000000
VERSION     DASVES010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644146"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(517..1608)
                     /locus_tag="VF240_18915"
     CDS             complement(517..1608)
                     /locus_tag="VF240_18915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7177337.1"
                     /translation="MTLVASLLAPAAQTQSNTRPRKTQPQQHSQTTPAQKKDSAPAEN
                     QAPARQQQQQAVDDDEVITVNSLEVLLPVTVRDAEGRLVPALTREDFVVREDGRAQPL
                     SDLSLRQVPVDVVLMVDSSSSVAASFDDFRRAVGEFATRLAPDDRISLIKFDDRVELL
                     QDWTQSHTQLRRALRRLTPGVFTRFNDALYLAAREQFTPGQRRRAVVVLSDGIDSGRG
                     AATLESVLRALLESQAALYAISNTQIEGARKRRELDTLLSGTEATVRFNDLRIGDLRE
                     SLRVLESSESNLADLARATGGRLYTPASFDALDSVYTEVAEELRSQYALYYTPLNAAR
                     DGRFRRVEVTTADPGHRATTRIGYYAPKR"
     gene            complement(1763..2518)
                     /locus_tag="VF240_18920"
     CDS             complement(1763..2518)
                     /locus_tag="VF240_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177338.1"
                     /translation="MRHSASTPMLALALCAAASVAAFAQGPARRSESPLTNFSVVKLV
                     RAGFREKTVIAIIRTRPARFDLSPDRLIDLKKNGVSEKIILAMIGHDLDTTLVAAEDD
                     WGSDDSFFKQGMPGGKGGASDPGETNIFGSSGGSRGSVRQNGVGGGSEGETLTTGSAT
                     VRIIRPPAEGGAAAAPKLERTPTLTNDSVVELVEAGFSEGTIVRRIEQSPAEFDLAPE
                     KLSELRKRRVSEPVIAAMRSAMSDDAAPARPEN"
     gene            complement(2665..2895)
                     /locus_tag="VF240_18925"
     CDS             complement(2665..2895)
                     /locus_tag="VF240_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177339.1"
                     /translation="MRLRTALLSALILLAAASPAASQTGAGESSWTKFADAGFSALFP
                     SEPPPFTYREKSPWARIAGASSPSSRRATPAS"
     gene            complement(2915..3901)
                     /locus_tag="VF240_18930"
     CDS             complement(2915..3901)
                     /locus_tag="VF240_18930"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7177340.1"
                     /translation="MATHLSCKTGEELSGRRRPLARVVGRSLAGVGRLALTALAVCAF
                     ASVALGDQLRMSDGKLIEVDEAWEDAQGVWYRRGGVTHHVERSRVRAIERTPKPASES
                     KSFVEVKNATKTDEVLTVVAAAQLAQTVWIHLKGGAEMEVDEANETAEGVWYKRGNLT
                     IFIDRERVERVERMRAPADDVAMASGASKGARRERAWTTGSRGLDSLIRQNGSRHGVD
                     PYLIFLVMEHESHFNARAVSPVGARGLMQLMPGTAARFGVRNPHDPAQSIAAGTRYIK
                     QLLHMFNGRVDLALAGYNAGEGSVRKYGHRVPPYRETRNYVRRISARYQGGD"
     gene            complement(3879..5228)
                     /locus_tag="VF240_18935"
     CDS             complement(3879..5228)
                     /locus_tag="VF240_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177341.1"
                     /translation="MTHTTILTRLEMESMGPPAPEEWADTDVSEGFLTDLALKHVAQS
                     PEPTTASIADEMRLPRALVEELLRGLAREKMIEVKLQAAVGATRYSMLDRGWDRVARA
                     RSMCGYVGPAPVSLRDYAHMMRLQAVPAVPASPDSVRAAFRDLVLPDSLLQTMGCVVN
                     SRRSLFITGPPGTGKTAVAERINSSLPGHIWIPYAIEIDGQIIRVYDTHNHRQAPAAD
                     SPDDYDRRWILIERPLVIVGGELTLDDADLQWSEAARFYEAPFHMKSNGGTLVIDDFG
                     RQRVEPHDLLNRWIIPLERRVDYLTLHTGKKIEVPFEQLVVFSTNLDEKDLVDEAFLR
                     RMGYRARVEPPTPAAYVEIFKRQAHTRGIRVERPLLDHVLNNYIIEHRQMKACEPRDL
                     LDRATDICLFEGRPLELSAKLIDAAWRNYFGASHGFGNHDPGKRNTGELRDGDPLEL"
     gene            complement(5353..6330)
                     /locus_tag="VF240_18940"
     CDS             complement(5353..6330)
                     /locus_tag="VF240_18940"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177342.1"
                     /translation="MRNRTTIAIFLAFFMTIGFTALWSAEGDEQEGDVATNVVAGTTD
                     GPAGVDVEQPKKKGSRWGRIFKAPFKAVAKVFGRDGDSKKMERMTEGDAQRFESAGVM
                     RVDYQNGEKKEAAAGSAQEHLDRGRAHLEGGNLNEAITELSRASSLDPKLSRAHSLLA
                     VAYDRKGLHDRARDSYDRAVDVNEHDPQTLNNLGYSLYINGNYRAAVDKLKKAARLAP
                     ADARIHNNLALAQSRLGKYDDAYKSFSRAGGEFNGRLNVAALLERAGRDADAAEHLEK
                     ARRLEPTSTSVLGRLADVYARTGKHAEAQSARQALEVLKAGDTVAAGGQ"
     gene            complement(6346..7281)
                     /locus_tag="VF240_18945"
     CDS             complement(6346..7281)
                     /locus_tag="VF240_18945"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7177343.1"
                     /translation="MLLITISIFVCVSLGVMALYWLVFRPQSAATERLRRMGDSGLGG
                     AAVAVAPSLDEESQVSTLAERVAKPLQRLAPPSASEARKLQKKLMQAGYRAPASAIIF
                     RAVHLAAMAGFPAMVALGCAFLGRPLKSAMLWIAFSFVVGFALPRYVLSRMVKGRQLR
                     LRWGLADALDLMVVSIEAGLGLNAAIVRVGDELKDVHFDISEEFQLTNLEIRVGRERD
                     EALRNLAERTGVDDLRSLVAMLIQADRFGTSIAKAVRVYADSLRTKRRQRAEQAAQKA
                     AVKLLFPLAFFLFPTLFIAILGPAAINLIDTFGKM"
     gene            complement(7361..8323)
                     /locus_tag="VF240_18950"
     CDS             complement(7361..8323)
                     /locus_tag="VF240_18950"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7177344.1"
                     /translation="MIPFFVFLFCLFAVFGAYLLATRGSDARRHRLQKRLAETLLHSA
                     HADDEEIRLAREELMSEIPWLNRTMVRMQTATKLKHAIEQADLQITVTRLVMFSLMAA
                     MLAGLAASVMTISWMLVALAALVAGSVPFIHVYYKRKKRIHAFLENLPDALELMSRAL
                     SAGHAFTEALHMVSTEMPEPIATEFSKTYEEQNLGLSLKLALDNLADRMPLLDLRLCI
                     TAILIQRETGGNLAEILEKVSHTIRERFRILEDLKTLTTSSRMSAWILCGLPIGVAMM
                     VTYLNPDYMSVLWTDPRGHQLIAVACAMQIIGMLFVRKILRIKI"
     gene            complement(8425..>8974)
                     /locus_tag="VF240_18955"
     CDS             complement(8425..>8974)
                     /locus_tag="VF240_18955"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7177345.1"
                     /translation="QQPHVVRLETRPPNIEGRGEVTQRDLVRNALRMRPDRIVVGEVR
                     GGEAIDMLQAMNTGHEGSLTTIHANTPRDALARLETMIQMTGMRLSEKAMRQQVASAL
                     DLVIQATRLSDGTRRITSISEITGMEGDTITMQEIFQFERSGIDKEGRVVGRFRPTGV
                     RPRFAERLKLYGLTLPRVFFEE"
BASE COUNT         1463 a         2725 c         3150 g         1636 t
ORIGIN      
        1 cattcaaaac cgagaacatt acgcgccgct agtacagaaa gcaagcgcct atgaatgcct
       61 gaaggcgaca gcacacacgt cactcaagac gtttgtaccg tcgctctcac caaactccct
      121 tgtaatcctg ctgccgctaa aatcaaaagc tacagcgaca tcccggtgtg caatgcccat
      181 gccatccgaa gccatcttca gaatagccat aaatacaaca cgttattaga cgatcccgtt
      241 tttcacagag ccatgcgacg cgttgatgtc gagtcaatta tacaccaatt tacgtatgga
      301 acacgagcag cccccttttg acacgcacag agtctggaac gtttctgaac cggcctgttt
      361 tgactcccat atttatcaaa agtcaggtat tccgcgtatt ttacgatgtc tcgacgacga
      421 accgccgccg actccaaagg atgccggcgg cggtttcagg gcatttattg attcaggctg
      481 ttcttttgct ttatctgagt cgcttccgct gaggcgctac cttttgggcg cgtaataacc
      541 gatgcgcgtc gtggcccggt ggccggggtc ggcggtggtg acttcgacgc ggcggaatct
      601 gccgtcgcgt gcggcgttga gcggcgtgta gtagagggcg tactggctgc gcaattcctc
      661 ggcgacctcc gtgtagacgg agtcgagcgc gtcgaaactg gcgggcgtgt agaggcgccc
      721 gccggtggcg cgggcgaggt cggcaaggtt gctctcgctc gactccagca cgcgcaacga
      781 ctcgcgcagg tcgccgatgc gcaaatcgtt gaagcggaca gtggcttccg tcccggagag
      841 cagagtgtcg agttccctac gcttgcgcgc gccctcgatc tgcgtgttgc tgatggcgta
      901 gagggcggcc tgagattcga ggagggcgcg cagcacagac tccagcgtgg cggccccgcg
      961 tccgctgtcg atgccgtcgg agagcacgac gacggcgcgg cgtctctggc cgggcgtgaa
     1021 ctgttcgcgc gccgcgaggt agagagcgtc gttgaagcgc gtgaagacgc cgggggtgag
     1081 ccggcgcagc gcacggcgca gttgcgtgtg gctctgcgtc cagtcctgaa gcagctcgac
     1141 gcggtcgtca aattttatca ggctgatgcg gtcatcgggc gcgaggcgtg tggcgaactc
     1201 accgacggcg cggcggaagt cgtcaaagct cgcggcgacg gacgaggatg agtccaccat
     1261 cagcaccacg tcaacgggca cttgccgcag cgacaggtca ctgagcggct gcgcgcggcc
     1321 gtcctcgcgc acgacgaagt cctctcgcgt gagcgcgggc acgaggcgcc cttcggcgtc
     1381 gcgcacggtg acggggagga gcacttcgag ggagttgacg gtgatgacct cgtcgtcatc
     1441 gactgcctgt tgttgttgct gtcgggccgg cgcttgattt tccgcaggag ctgaatcttt
     1501 tttctgagcc ggagttgtct gagagtgttg ctgtggctgg gtcttgcgcg ggcgggtgtt
     1561 gctttgcgtt tgagcggcgg gagcgagcag tgaggcgaca agcgtcactg cgagtagcga
     1621 taggccgata aacttaaccg gggcgggctg tgtccgttcc gcctccgaag aacccgttgc
     1681 acttgatacg cgacggccgc gcggacgagt cacttcgccg cgcgtctctg aaacaaacgt
     1741 gtcactcccc ttgttcatgc gctcagttct ccggtctggc gggggccgcg tcgtcgctca
     1801 tggcggagcg catcgccgcg atgaccggct cggagacgcg gcgcttgcga agctcggaga
     1861 gcttttcggg agcgaggtcg aattccgcgg gcgactgctc gatgcggcgg acgatggtgc
     1921 cctcgctgaa gccggcctcg accagctcga cgaccgaatc gttggtgagc gtgggcgtcc
     1981 gctcaagctt cggcgcggcc gcggcgccgc cctcggcggg cggtcggatg atgcggacgg
     2041 tggcgcttcc ggtggtcagc gtttcgccct cgctgccgcc gccgacgccg ttctggcgga
     2101 cgctgccgcg cgaacctccc gacgagccga agatgttcgt ctcgccgggg tcgctggctc
     2161 cgcctttgcc gcccggcatg ccctgtttga agaaggagtc gtcgctgccc cagtcgtcct
     2221 cggcggcgac gagcgtcgtg tcgaggtcgt ggccgatcat cgcgaggatt attttctcgc
     2281 tgacgccgtt cttcttgagg tcgatgagac ggtcgggcga gaggtcgaag cgcgcggggc
     2341 gcgtgcggat gatggcgatg acggtcttct cgcggaagcc tgcgcgcacg agcttgacga
     2401 ccgagaagtt agtcagcggc gactcggagc ggcgcgccgg cccctgcgcg aaggccgcga
     2461 cggacgcggc ggcgcacagc gcgagcgcga gcatgggtgt cgatgcactg tgtctcattc
     2521 gtttccatcc ttggcggagt tgagtccgtc atggctccct gatctcgaag gaatcgagga
     2581 accgctcgcc ctcgcgtgaa ggggacgcat cattgtagcg aaagaatgcg acggtgtaga
     2641 agcgtttctc ggtcagatac attctcagga cgccggtgtt gcgcggcgac tcgacggcga
     2701 actcgcgccc gcgatgcgcg cccagggaga tttctctctg taggtgaacg gcggcggctc
     2761 ggacgggaag agtgcggaga aaccggcgtc ggcaaacttc gtccacgaag actcgcccgc
     2821 tcccgtttgc gacgcggcgg gcgatgccgc cgccagaaga atcaacgctg agagcagtgc
     2881 tgttcttagt ctcatgcgtt cggccggcac atcgtcaatc tccgccctga tagcgcgcgc
     2941 tgatgcggcg cacgtagttg cgcgtctcgc gatagggcgg gacgcggtgg ccatacttgc
     3001 gaaccgagcc ttcgccggcg ttgtagcccg cgagcgcgag atcgacgcgt ccgttgaaca
     3061 tgtggagcaa ttgcttgatg tagcgtgtcc ccgcagcgat gctctgcgcc gggtcgtgcg
     3121 ggttgcgcac gccgaagcgc gcggccgtcc ccggcatgag ctgcatcagc ccgcgcgccc
     3181 cgacggggct gacggcgcgc gcgttgaagt gcgactcatg ctccatcacg aggaagataa
     3241 ggtaagggtc aacgccgtgg cgagagccgt tctgacggat gagtgaatcg aggccgcggc
     3301 ttccggtcgt ccacgcgcgc tcgcgcctcg cgcccttcga cgcgccgctg gccatcgcca
     3361 cgtcgtcggc cggcgcgcgc atgcgctcga cgcgctcgac gcgctcgcgg tcgatgaaga
     3421 tcgtcaggtt gccgcgcttg taccagacgc cctcggcggt ctcgttcgcc tcgtcaacct
     3481 ccatctcggc cccgcccttc aggtgtatcc agacggtctg cgccaactgt gccgccgcga
     3541 ccaccgttaa cacctcgtcc gtctttgtcg cgttcttaac ttcgacaaat gacttcgact
     3601 cactcgcggg cttcggcgtg cgctcgatgg cgcggacgcg cgagcgctcg acgtggtgcg
     3661 tcacgccgcc gcgtctgtac cagacgccct gcgcgtcttc ccaagcctcg tctacctcga
     3721 tcagcttgcc gtcgctcatc cgcaactggt cgccgagcgc gaccgaagcg aaggcgcaga
     3781 cggcgagcgc cgtgagcgcg agacggccga cgcctgcgag cgagcggcca acgacgcgag
     3841 ccagaggacg ccttcgtccc gacaattctt cgccggtctt acagctcaag tgggtcgcca
     3901 tcgcgtaatt cccccgtgtt gcgtttgccg gggtcgtggt tgccgaaccc gtgcgaggct
     3961 ccgaagtagt tgcgccaggc ggcgtcgatg agtttcgccg acagctccag cgggcggccc
     4021 tcgaagagac agatgtcggt ggcgcggtcg agaaggtcgc gcggctcgca cgccttcatc
     4081 tggcggtgct cgatgatgta gttgttgagg acgtggtcga gcagcggacg ctcgacgcgg
     4141 atgccgcgcg tgtgcgcctg ccgcttgaag atttcgacgt aggcggccgg agtcggcggc
     4201 tcgacgcgcg cgcggtagcc catgcgacgc aggaacgcct cgtcaaccaa gtccttctcg
     4261 tcgaggttcg tggagaagac gacgagctgc tcgaagggca cttcgatctt cttgccggtg
     4321 tggagcgtca ggtaatcgac gcgtcgctcc agcgggatga tccagcggtt caaaaggtcg
     4381 tgcggctcga cgcgctggcg accgaagtcg tcgatgacga gcgtgccgcc gttggacttc
     4441 atgtggaagg gcgcttcgta gaagcgcgcg gcctcgctcc actggaggtc ggcgtcgtcg
     4501 agcgtgagtt cgccgccgac gatgacgagc gggcgctcga tgagaatcca gcggcggtcg
     4561 tagtcgtcgg gcgagtcggc ggcgggcgcc tgccggtggt tgtgagtgtc gtagacgcgg
     4621 ataatctggc cgtcgatctc gatggcgtag ggaatccaga tgtggccggg cagactcgag
     4681 ttgatgcgct cggcgacggc cgtcttgccg gtgcccggag ggccggtgat gaagagtgag
     4741 cgacgcgagt tgacgacgca acccatcgtt tgcaacagcg aatcggggag cacgaggtcg
     4801 cggaaggcgg cgcgcaccga gtcgggcgag gcgggcacgg ccggcaccgc ctgaaggcgc
     4861 atcatgtgcg cgtagtcgcg cagggagacg ggcgcggggc cgacgtagcc gcacatggag
     4921 cgggcgcgcg cgacgcgatc ccagccgcgg tcgagcatcg agtagcgggt cgcgcccacg
     4981 gccgcctgta gcttcacctc gatcatcttc tcgcgcgcga ggccgcgcaa cagctcctcg
     5041 accagagcgc gcggcagacg catctcgtcg gcgatggacg ccgtcgtcgg ctccggcgac
     5101 tgcgcgacgt gcttgagggc gaggtcggtc aggaagccct cggacacgtc cgtgtcggcc
     5161 cactcttccg gcgccggcgg ccccatgctc tccatctcaa gccgcgtgag aatcgtcgtg
     5221 tgcgtcatcg gttcctcgta ggacaggcat tcctgcctgt cattcctgtc attacttccg
     5281 gcgtcatttc aatcgggcat gatgtagagc ctgcctgcgg caggctgaca ggcaggaatg
     5341 cctgtcctac gtttactgcc cgcccgccgc gaccgtgtcg ccggccttga ggacttcgag
     5401 cgcctgccgg gccgactgcg cctcggcgtg cttgccggtg cgtgcgtaaa cgtcggcgag
     5461 gcggccgagg acgctcgtcg aagtgggttc gagccggcga gccttctcaa ggtgctcggc
     5521 ggcgtcggcg tcgcggccgg cgcgctcaag gagggcggcg acgttgaggc ggccgttgaa
     5581 ctcgccgccc gcgcgggaga agctcttgta tgcgtcgtcg tacttgccga ggcggctctg
     5641 ggcgagggcg aggttgttgt ggatgcgcgc gtcggcgggc gcgagccggg ccgccttttt
     5701 gagcttatcg acggcggcgc ggtagttgcc gttgatgtag agcgagtagc cgaggttgtt
     5761 gagggtctgc gggtcgtgct cgttgacatc gacggcgcgg tcgtaggagt cgcgcgcgcg
     5821 gtcgtggaga cccttgcggt cgtaggcgac ggcgagcagg ctgtgggccc gactcagttt
     5881 ggggtcgagc gaggaggcgc gcgaaagttc cgtgatggcc tcgttgagat tgccgccttc
     5941 gaggtgcgcg cggccccggt cgaggtgttc ctgcgcggag ccggccgccg cttccttttt
     6001 ctcgccgttc tggtaatcga cgcgcatcac gcccgcgctc tcgaaccgct gcgcgtcgcc
     6061 ctcggtcatg cgctccatct ttttgctgtc gccgtcgcgg ccgaagactt tcgccacggc
     6121 tttgaagggc gccttgaaga tgcggcccca gcgactcccc ttcttcttcg gctgctcgac
     6181 atcgacgccg gcggggccgt cggtcgtgcc ggcgacgacg ttcgtcgcca cgtcgccctc
     6241 ctgctcgtcg ccctcggcgc tccagagcgc ggtgaaaccg atggtcatga agaaagcgag
     6301 aaagatcgcg atggtcgttc tgttgcgcat gggtcgtaaa cctccctaca tcttgccgaa
     6361 cgtgtcgatg aggttgatgg ccgcggggcc gaggatggcg atgaagagcg tggggaacag
     6421 gaagaacgcg agcgggaaca gcaacttcac cgccgccttc tgcgcggcct gctcggcgcg
     6481 ctgccggcgc ttcgtgcgca ggctgtcggc gtagacgcgc acggccttcg cgatggaggt
     6541 gccgaagcgg tcggcctgaa tgagcatggc gacgaggctg cggagatcgt caactcccgt
     6601 gcgctcggcg agatttctga gcgcctcgtc gcgctcgcgc ccgacgcgta tctccagatt
     6661 cgtcagttgg aactcctcgc tgatgtcgaa gtgaacgtcc ttcaactcgt cgccgacgcg
     6721 gacgatggcc gcgttgaggc cgagtccggc ttcgatggag acgaccatca ggtcgagcgc
     6781 gtcggcgagg ccccagcgga ggcggagctg gcggcccttg accatgcggg agagcacgta
     6841 acgcggcaga gcgaagccga cgacgaagga gaacgcgatc cagagcatcg cgctcttgag
     6901 cggacggccc aggaacgcgc agcccagcgc caccatcgcc gggaagccgg ccatcgccgc
     6961 cagatgcacc gcgcggaaga tgatggccga cgcgggcgcg cggtagccgg cctgcatcaa
     7021 cttcttctgg agcttgcgcg cctccgaggc cgacggcggc gcgagacgct ggagcggctt
     7081 ggccacgcgc tcggcgagcg tcgagacctg cgactcttcg tcgagcgacg gggcgaccgc
     7141 cacggccgcg ccgcccagcc ccgagtcgcc catccgccgc aggcgctccg tggccgcgct
     7201 ctgcggccgg aacacgagcc agtagagcgc catcacgccg agcgagacgc acacgaatat
     7261 ggagatggtg atgagaagca tagtgataag tgatgagtga taagtgatga gcaggaagtg
     7321 atttttctta tcacttatca ctcatcactt atcacgactc ctaaatcttg atccgaagaa
     7381 tcttccgaac gaagagcatg ccgatgatct gcatggcgca ggcgacggcg atgagctggt
     7441 ggccgcgcgg gtcagtccag aggacgctca tgtagtcggg gttcagatac gtgaccatca
     7501 tggcgacgcc gatggggagg ccgcagagaa tccaggccga catgcgcgag gaggtcgtca
     7561 gcgtcttcag gtcttcgaga atgcggaagc gttcgcggat ggtgtgggag accttctcca
     7621 gaatctcggc gaggttgccg cccgtctcgc gctggatgag gatggccgtg atgcagaggc
     7681 gcaggtctaa gagcggcatg cggtcggcga ggttgtcgag ggcgagcttg agggagagac
     7741 cgaggttttg ctcctcgtag gtcttgctga actcggtggc gattggctcg ggcatctcgg
     7801 tcgagaccat gtgcagcgcc tcggtgaagg cgtggccggc ggagagggcg cggctcatca
     7861 gctccagcgc gtcgggcagg ttttcgagga aggcgtggat acgtttcttc cgcttgtagt
     7921 agacgtggat gaagggcacg gagccggcga cgagcgcggc gagggcgacg agcatccatg
     7981 agatcgtcat caccgacgcg gcgaggccgg cgagcatggc cgccatcagc gagaacatca
     8041 cgagtcgcgt gaccgtgatc tgaaggtcgg cctgctcgat ggcgtgcttc aacttcgtgg
     8101 ccgtctgcat ccgcaccatc gtgcggttga gccacggtat ctcgctcatc agctcctcgc
     8161 gcgcgaggcg tatctcctcg tcgtcggcgt gagcggagtg gagcagggtt tcggccagac
     8221 gcttttgcag gcggtgccgg cgcgcgtccg agccacgcgt cgccaaaaga tacgcgccga
     8281 agacggcgaa gaggcagaac aggaatacga agaaggggat cataatagta agcagtaagc
     8341 agtaagcagt gagcagtcag tcgaggcgac gcgggcggtg ggcagtgaac agttttttac
     8401 tgcttactgc tcaccgctca ctgctcactc ctcgaaaaag acgcgcggca gcgtcaggcc
     8461 gtagagcttg aggcgctcgg cgaagcgcgg gcggacgccc gtggggcgga agcggccgac
     8521 gacgcggcct tccttgtcga tgccgctgcg ctcgaactgg aaaatctcct gcatggtgat
     8581 cgtgtcgcct tccatgccgg tgatctcgga gatggaggtg atgcggcgcg tgccgtcgga
     8641 caggcgcgtg gcctgtatga cgaggtcgag ggccgaggcg acctgctggc gcatggcctt
     8701 ttcagagagg cgcatgcccg tcatctgaat catcgtctcc agccgcgcca gcgcatcgcg
     8761 cggcgtgttg gcgtggatgg tcgtgagcga gccttcgtgg ccggtgttca tggcctgcag
     8821 catgtcgatg gcttcgccgc cacgcacctc gccgacgacg atgcggtcgg ggcgcatgcg
     8881 cagggcgttg cggacgaggt cgcgctgcgt gacctcgccg cgcccttcga tgttgggcgg
     8941 gcgcgtctcg aggcggacga cgtgcggctg ctgg
//
LOCUS       DASVES010000456        10729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_281093, whole genome shotgun sequence.
ACCESSION   DASVES010000456 DASVES010000000
VERSION     DASVES010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281093"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VF240_18960"
     CDS             complement(<1..835)
                     /locus_tag="VF240_18960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7177346.1"
                     /translation="MSGEGLREQESAVTSLAERLRVQIRRAGPMTFHDWMRAALYDPA
                     EGYYQRRGHTRWGRAGDYRTAPETTPLFAATFARHFASLHEELHSPTQLTIVEAGAGA
                     GHFARGVLTTLRRDHPKVFSSVRYVVDELSADSRARAAELLAPFAGRIEFRRIEECRE
                     PFEAGIIFSNELLDAFPVHRVVSRGGELRELCVGLDDRGRFSWVEGEASTPKLAAHFR
                     AGSVTLSEGQIAEVNLDAELWVARAASVLRRGFVITVDYGAEATELYRAPGRASGTLR
                     GY"
     gene            complement(879..1442)
                     /locus_tag="VF240_18965"
     CDS             complement(879..1442)
                     /locus_tag="VF240_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7177347.1"
                     /translation="MFCPHCGQRQVSNEVRFCAACGFQMNMVSQLLATGGTKVQVGTT
                     GPRKLSPRQKGIRQGVMLMLSTMLIVPLVAIIGVALFDLPGEIAGIAAVTCFIGGLLR
                     IIYALLLEDREPAFEPSPQLTSYAPPATPLFMNQPAARSSSLPPQQQQQGIPVSYRPP
                     ADTGELRRPPSVTENTTRLLKDRPEDK"
     gene            complement(1489..2052)
                     /locus_tag="VF240_18970"
     CDS             complement(1489..2052)
                     /locus_tag="VF240_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7177348.1"
                     /translation="MYCPHCSQQQISPEVRFCSRCGFPLDGVTALLANGGLLPGQQQA
                     GGELSPPSPRLQGVRQGGLLILMGMFLVPFLAFMAEGLNIIPEEVAMAAALICFLGGF
                     LRIIYAMLFEDGPLRRKTLPAGYVNPQAAMPWATGGVGRDAAALPPAQSVPANAYVPP
                     RADTSEITPRPPSVTEHTTKFLDKQGR"
     gene            complement(2168..2677)
                     /locus_tag="VF240_18975"
     CDS             complement(2168..2677)
                     /locus_tag="VF240_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177349.1"
                     /translation="MTRGREAPDPSTMQIIRSLDGQYASLHLRSCGLVRAVPEGKLYW
                     QPRPSSGDFPNYSCGEYLLRSAAVVEQTFGGVTANLWDDPFEWTLPEALSTSAHVEEY
                     LNEAEQARLRAFALFHADADLLKEIATPAGMRTLYSLALETLVRASHYQGRAFATFRL
                     FSDTPLPRL"
     gene            complement(2677..3024)
                     /locus_tag="VF240_18980"
     CDS             complement(2677..3024)
                     /locus_tag="VF240_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177350.1"
                     /translation="MRQLRRTLFFSTLFILLLSPVAFAKTTINKINADPGRYHNKKVT
                     IVGTVTDSYGVFTEGGYEIDDGTGKIWVLADRAVPARGARVEVKGRVYTGIVFHGRNM
                     GTAIRETGRKAKD"
     gene            complement(3254..4759)
                     /locus_tag="VF240_18985"
     CDS             complement(3254..4759)
                     /locus_tag="VF240_18985"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7177351.1"
                     /translation="MSSTQTAVDETPRKYQLFIDGKWVDAESGKTFTTPNPSTGATLA
                     EVAEADKADIDKAVAAARRAFEGKWSKISARDRGRMMYKLSQLIESKSAELAHLETSD
                     NGKPIKESMYVDLPQVVENFEYFAGWATKIEGETIPVPGQMFNYTLREPIGVCGQIIP
                     WNFPLLMAAWKLAPALAAGNTVVLKPAEQTPVGAMELGKLIQEAGFPDGVVNIVPGYG
                     ETAGAALASHPDIDKVAFTGSTEVGKIIAHAAADNLTKVSLELGGKAPNIVFADADIE
                     QAVNGAMMGIFFNQGQVCCAGSRLFLDERIKDEFLTLFKEKAEKIKVGDPLDKATQMG
                     PQVSGEQLDRIKGYVDIARGEGATVFSGGCPPQLEGDFQKGFFFQPTIFSEVNNKMRV
                     AQEEIFGPVTSVITFTDEADLVKQANDTIYGLSAGIWTKDITRAHSFAKQVKAGVVWI
                     NTFNMFNAASPFGGFKQSGYGREMGRHALDLYTQIKSVWVDLSGKPIGWFN"
     gene            complement(4896..6095)
                     /locus_tag="VF240_18990"
     CDS             complement(4896..6095)
                     /locus_tag="VF240_18990"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX7177352.1"
                     /translation="METFLYTAAGVCLLLLIAFDVYATVLHSSARYGPIGEGLNRMVW
                     RVARTVAFKLSRPSRHRVLNVIGPLLMPLLVVVFIGTLVVAFALIYFPRMPEQFILAN
                     PNASSDFIDSVYFSGVTLTSVGYGDILPRTTAMRFTAMIESAAGLGLISLAIAYLLTI
                     YRALERKRVAALAFYHQAGEGADAAGYIAYYFVEGRFYGLREELRTASRDLQELLEAH
                     VEHPVIHYFHPVEVYKGLPRMLFLLLEVCTVIRTSLDMDEYSDLRNFPEVRVMEANTR
                     QVLNELVRTLDLERRRNEREEHRPLDEDRWRRRFDRNLRRLAEAGIKTRDGQNEDGFD
                     HYREQRKEWEAKLQRLSNYLGYDWDEVTGDTDPEYASDEKKEESLGAPSGETGAKGGR
                     RANDGRR"
     gene            complement(6215..9127)
                     /locus_tag="VF240_18995"
     CDS             complement(6215..9127)
                     /locus_tag="VF240_18995"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX7177353.1"
                     /translation="MTHNLFNTLQTFKTAGGREGQFYSIPQLEREGVGPVSRLPVSMR
                     IVLESILRNFDGHVVEESDVRALANWGAQAERDEEIPFYVARVLLQDFTGVPLLVDLA
                     AMRSAVERMGRDPGIIEPLVPVDLVIDHSVQVDFWSSADALRLNMETEFKRNRSRYQF
                     LKWGTQAFNNFRVVPPGIGIVHQVNLEYLAQGVLEKEGVYYPDTLVGTDSHTTMINGL
                     GIVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGALKEGVTATDLVLRVTELLRQAK
                     VVGKFVEFHGEGASSLPVTDRATIANMAPEYGATMGFFPVDEKTCEYLAATGRTPEQI
                     EIVRSYFKAQEMFGIPAAGQCDYSTLHELDLADIKPSVAGPKRPQDRIELGDLKEKFL
                     DLLQKPIGENGYNRAKEEIDRRFRVKIGVSEPTRSLPILGGGGQEQETAPETVATETV
                     GGLGSVSEKDTSAHTELEMMNNRPTPDRLVEIPHEEFPSATVDLGHGDVLIAAITSCT
                     NTSNPSVMLAAGLLAKKAVERGLRVRPGVKASLAPGSRVVTEYLERAGLQKYLNELGF
                     NLVGYGCTTCIGNSGPLDPRIEAAVNDNDLVAASVLSGNRNFEARVHQSIRANFLMSP
                     PLVVAFALAGRINVDLTSEPLGKGSNGDGDVYLKDIWPTLEEISALMSTATNPKTYRM
                     LYESFADANPMWNEIPSVAGEVYQWESQSTYIQEPPYFEKFSMQPGEFCDIIGARPLA
                     IFGDSVTTDHISPAGAIKAKSPAGRYLLLRGVSVEDFNSYGSRRGNHQVMLRGTFANV
                     RIKNQMVPGTEGGVTCLHPVSEQMSIYDASVNYQAAGIPLVVIAGQEYGTGSSRDWAA
                     KGTRLLGVHAVIAESFERIHRSNLVGMGVLPLQFKEGANAQTLGLDGDEVFDIVGIGG
                     ADIRPRQDVTLRVRREDGAEQEVPLTLRIDTPIEIEYYRHGGILQYVLRQLLAQSA"
     gene            9346..9981
                     /locus_tag="VF240_19000"
     CDS             9346..9981
                     /locus_tag="VF240_19000"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX7177354.1"
                     /translation="MTDKISSATPTPIAAPGAFVRTVSIDTFLTGLRSIRESEFTVGR
                     VFDYLKRHPVEANTLSRYQFFSRNHYTRNLIFKNPLFELVAVCWDIGQGSQIHDHHDQ
                     NCWMSMPVGRLRVQNYHVAERDERRSYARIEPTDSFDIHRLAPAAEVDPEEPVHKVSN
                     LPEFKQRAVSLHIYSRPFNTCVVYSPAKNDYRVQPLTYHSEYVRLCEGVRL"
     gene            10149..10547
                     /locus_tag="VF240_19005"
     CDS             10149..10547
                     /locus_tag="VF240_19005"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HEX7177355.1"
                     /translation="MPKKNPEDFTYLSNDPNYVPDPEIGAFMEEEISRAPKDSDHLAQ
                     RLRNSTSASPSDAGGDIDASWEDVNDSGTESVSGDNPTPDQSDVEENAHAIGVNFEDN
                     EELEVIDKIERRDRDRFELDPRSKTEDNSI"
BASE COUNT         1904 a         3336 c         3519 g         1970 t
ORIGIN      
        1 cgtagcctct caaggtgccg cttgcgcggc ccggcgctcg ataaagctct gtggcctcgg
       61 cgccgtaatc gacagtgatg acgaagccgc gccgaaggac tgaggcggcg cgcgcgaccc
      121 acagttcagc atcgagattc acttcggcga tctgcccctc agacaaggtg acgctccctg
      181 cgcggaagtg cgccgcgagt ttcggagtgg aggcttcgcc ctcgacccat gagaatcggc
      241 cgcggtcgtc cagtccgacg cagagttcgc gaagctcgcc gccgcgtgag acgacacggt
      301 ggacggggaa ggcgtcgagc agctcgtttg agaaaatgat gcccgcctcg aaaggctcgc
      361 ggcactcctc gatgcgccgg aattcgatgc gcccggcgaa tggggcgaga agctcggccg
      421 cccgcgcgcg cgagtcggcg ctcagttcat cgacgacgta gcgcacggac gaaaaaactt
      481 tcggatgatc gcgccgcaac gtggtgagga cgccacgcgc gaagtggccc gcgccggcgc
      541 ccgcctcgac gatggtcagt tgggtcggag agtgtagttc ttcgtggaga gaagcgaagt
      601 ggcgtgcgaa ggtggcggcg aagaggggcg tcgtctcggg ggcggtgcga tagtcgccgg
      661 cgcggcccca gcgtgtgtgg ccgcgcctct ggtagtagcc ctctgcgggg tcgtagagcg
      721 cggcccgcat ccagtcgtgg aaggtcatcg gcccggcgcg tcggatttga acgcgcaggc
      781 gctccgcaag ggacgtgacg gccgactcct gctctctgag gccttcgccg ctcatctttt
      841 ttcacacacg attcgacggg ctgcgcggcg ggggcgactc acttgtcttc gggtcgatcc
      901 ttgaggagcc gcgtggtgtt ctcggtgacg ctcggcggcc ttcggagttc gcccgtgtcg
      961 gcgggcggtc ggtaggagac ggggatgccc tgctgctgct gctgtggcgg gagtgacgaa
     1021 gagcgcgcgg cgggctgatt cataaagagg ggcgtggccg gcggcgcgta ggatgtcaac
     1081 tgtggcgacg gctcaaaggc cggctccctg tcttcgagca atagcgcgta gatgatccgc
     1141 agaagcccgc cgatgaagca cgtgacggcg gcgatcccgg caatctctcc cggcaaatca
     1201 aacagggcga caccgatgat cgcgacgagc ggcacgatca gcatcgtgct cagcatcagc
     1261 atcacgccct gccggatacc cttctgccgc ggcgagagct tgcgcggccc cgtcgtccca
     1321 acctgtacct tcgttccacc cgtcgccagc agttgcgaca ccatgttcat ctggaagccg
     1381 cacgccgcgc agaagcgcac ctcgtttgac acctgcctct ggccgcagtg tggacagaac
     1441 attcgctctg ggtcctctcg aattaattga cgaagcagga gcgacggttc agcgcccctg
     1501 cttgtcgaga aacttcgtcg tgtgttcggt gacgctcggc gggcgcggcg tgatctccga
     1561 cgtgtccgcg cgcggcggca cgtaggcgtt agcgggcacg ctctgcgcgg gcgggagcgc
     1621 cgccgcgtcg cgtcccacgc cgcccgtagc ccacggcatc gcggcctgcg ggttcacgta
     1681 accggccggg agcgtcttcc ggcgcagcgg gccgtcctcg aacagcatcg cgtagatgat
     1741 gcggaggaag ccgccgagga agcagatgag cgccgccgcc atcgccacct cctcgggaat
     1801 gatgttcaac ccttcggcca tgaaggcgag gaatggcacg aggaacatcc ccatgagtat
     1861 cagcaggcca ccctgccgca cgccctgcag gcgcggcgat ggcggcgaca gctcgccgcc
     1921 cgcctgttgt tggccgggga gcaggccgcc gttggcgagc agcgccgtca cgccgtcgag
     1981 cgggaagccg cagcgcgaac agaagcgcac ctccggggat atctgctgct ggctgcaatg
     2041 tgggcagtac atctcggaca agacctcgac gcgatagaat ggtcgccacg acggacgcgg
     2101 ctcttagacc catgaggcgt ttacgaatcg acccgccgca aagttcgcgt tccagtcgga
     2161 aacgatatca caggcggggc agtggcgtgt cggaaaaaag tcggaaggtg gcgaaagccc
     2221 gtccctgata gtgcgacgcg cggacgagcg tttcgagcgc gaggctgtag agggtacgca
     2281 tcccggcggg cgtcgcgatc tctttcagca ggtcggcgtc ggcgtggaag agggcgaagg
     2341 cgcgaaggcg cgcttgctcg gcttcgttca gatactcctc gacgtgtgcg ctcgtcgaga
     2401 gggcttcggg tagcgtccac tcgaaggggt cgtcccagag gttcgcggtg acgccgccga
     2461 acgtctgctc gacgaccgcc gccgagcgca gcaaatactc cccgcacgag tagttgggga
     2521 agtcgccgga ggaggggcgg ggctgccagt aaagtttacc ttcggggacg gccctgacaa
     2581 gcccgcacga gcgcaggtgc aggctcgcgt attgcccatc gagactacgg atgatctgca
     2641 tcgtcgaggg gtcgggcgct tcccgccccc gcgtcattag tccttcgcct tgcgtccggt
     2701 ttcccgaatg gccgtcccca tgttgcggcc gtggaagacg atgcccgtgt agacgcggcc
     2761 cttgacctcg acgcgggcgc cgcgcgcggg aaccgcccgg tcggcgagca cccagatttt
     2821 gcccgtcccg tcgtcgatct cgtacccgcc ctcggtgaac acgccgtagc tgtcggtgac
     2881 ggtcccgacg atggtcactt tcttgttgtg atagcggccg gggtcggcgt tgattttatt
     2941 gatggtcgtc ttggcgaagg cgaccggaga cagcaggagg ataaagaggg tgctgaaaaa
     3001 aagtgttcgg cgaagctgac gcatggcgtc tcctttcaaa agtcactgat tgctcagagt
     3061 ttctatggga gagcttgaga taatcctagc atgcgatgac gcgggcgccg aaaaaggatg
     3121 ccaggaaaag taaggatgaa ggatgaataa aagccgtaaa tcgtaaatcg tcaatcgtga
     3181 atagaagaaa ctgccttcca cgattcacga ttgacgattt acgattcacg gtcttgctca
     3241 tccctcatcc ttgctagttg aaccagccga tgggcttgcc ggagagatcg acccagacgc
     3301 tcttgatctg cgtgtagagg tcgagggcgt ggcggcccat ctcgcggccg tagccggact
     3361 gcttgaagcc gccgaagggc gaggcggcgt tgaacatatt gaaggtgtta atccagacga
     3421 cgccggcctt aacctgtttg gcaaagctgt gggcgcgcgt gatgtccttc gtccagatgc
     3481 cggccgacag accgtagatg gtgtcgttgg cctgtttgac gaggtcggcc tcgtccgtga
     3541 aggtgatgac ggaggtgacg gggccgaata tctcttcctg cgccacgcgc atcttgttgt
     3601 tcacttcgct gaagatggtc ggctggaaga agaagccctt ctggaagtcg ccttcgagct
     3661 gcggcgggca gccgccggag aagacggtcg cgccttcacc gcgcgcgatg tccacgtagc
     3721 ccttgatgcg gtcgagctgt tcgccggaaa cctgcggccc catctgcgtc gccttgtcga
     3781 gagggtcgcc gactttaatc ttctcggcct tctccttgaa gagtgtcagg aactcgtcct
     3841 tgatgcgctc gtcgaggaag aggcgcgagc cggcgcagca gacctgaccc tgattgaaga
     3901 agatgcccat catcgcgccg ttgacggcct gttcgatgtc ggcgtcggcg aagacgatgt
     3961 tgggggcctt gcctccgagt tcgagcgaga ctttggtcag gttgtcggcc gcggcgtggg
     4021 cgataatctt gccgacctcg gtcgagccgg tgaaggcgac tttgtcgatg tcggggtgcg
     4081 aggcgagcgc ggcgccggcc gtctcgccgt agccggggac gatgttgacg acgccgtcgg
     4141 ggaagccggc ctcctgaatg agcttgccga gttccatcgc gcccacgggc gtctgctcgg
     4201 caggcttcaa gacgacagtg ttgccggccg cgagcgcggg cgcgagtttc cacgcggcca
     4261 tcagcagcgg gaagttccac gggatgatct ggccgcagac gccgattggc tcgcgcagcg
     4321 tgtagttgaa catctgtccg ggcacgggga tggtctcgcc ctcgatcttc gtcgcccagc
     4381 cggcgaagta ctcgaagttc tcgaccacct gcggcagatc gacgtacatc gactccttga
     4441 tgggcttgcc gttgtcggag gtttcaaggt gggccagctc cgccgacttc gactcgatga
     4501 gctgcgaaag cttgtacatc atccggccac ggtcgcgggc ggagattttc gaccacttgc
     4561 cctcgaaggc gcggcgcgcg gcggcgaccg ccttgtcgat gtcggccttg tcggcctcgg
     4621 ccacttcggc gagggtcgcc cccgtcgagg ggttgggcgt cgtgaaggtc ttgccggact
     4681 cggcatcgac ccacttgccg tcgatgaata gttgatactt gcgaggcgtc tcgtctacgg
     4741 ccgtctgtgt ggatgacata aaagtgttac tccttaatga acggtggatg tggattcttt
     4801 gggcttcgct gtcgccagga gatgtgtgcg tccctactct atcacccgcc gcgcgcgcgc
     4861 cccaagccgc cgggcgcgtg cgcgcgcgcg ggcgtttagc gccggccgtc attggcgcgt
     4921 ctcccgcctt tcgccccggt ctcgccggag ggcgcgccga gtgattcttc tttcttttcg
     4981 tcggaagcgt actcggggtc ggtgtcgccg gtgacttcgt cccagtcgta gccgagatag
     5041 ttcgagagac gctggagctt ggcctcccac tccttgcgtt gctcgcggta atggtcgaag
     5101 ccgtcctcgt tttgtccgtc gcgtgtcttg atgccggcct cggcgagacg ccggaggttg
     5161 cgatcaaaac gacgccgcca gcggtcttcg tcgagcgggc gatgctcttc acgctcgttg
     5221 cgacgccgct ccaaatcgag cgtgcgaaca agctcgttca agacctgccg ggtgttggcc
     5281 tccatcacgc gcacctcggg gaagttgcgc aggtcggagt attcgtccat gtcgagcgag
     5341 gtgcggataa cggtgcagac ttcgagcagg aggaagagca tccgcggcag gcccttgtag
     5401 acctcgacgg ggtggaagta gtggatgacc gggtgctcga cgtgcgcctc aagcagctct
     5461 tgcaaatcgc gcgaggcggt gcgcagctct tcgcgcagac cgtagaagcg cccctcgacg
     5521 aagtagtagg cgatgtagcc ggcggcgtcg gccccctcgc cggcctgatg gtagaaggcg
     5581 agcgccgcga cgcgcttgcg ctccagcgca cggtagatgg ttagcaggta ggcgatggcc
     5641 agagagatca gccccagccc cgcggccgac tcgatcatcg ccgtgaagcg catggccgtc
     5701 gtgcgcggca agatgtcgcc gtagccgacc gaggtcagcg tgacgccgct gaagtagacc
     5761 gagtcgatga agtcggagga ggcgttcggg ttcgcgagga tgaactgctc gggcatccgc
     5821 gggaagtaga tgagggcgaa ggcgacgacg agggtgccga tgaagacgac gacgaggagc
     5881 ggcatcagca gagggccgat cacattgagc acacggtgac gcgacggccg cgagagcttg
     5941 aaggcgacgg tgcgggccac acgccacacc atgcggttga ggccctcgcc tatcggcccg
     6001 taacgcgcgc tggagtggag caccgtcgcg taaacgtcga aggcgatgag caacaggagg
     6061 cagacgccgg cggccgtgta aagaaaagtt tccatgcaac aaggaagggg gaaggggtaa
     6121 agggtaaggg ttagggaaca agcgcgtgat tgtcctcggc cgcttcggtt ttacccttaa
     6181 cccttaccct ttaccccttc cccttttcct tctttcacgc ggactgcgcg agcaactgcc
     6241 ggagcacgta ttggaggatg ccgccgtggc ggtagtactc gatctcgatg ggcgtgtcga
     6301 tgcgtagcgt cagcggcacc tcctgctccg cgccgtcctc gcggcgcacg cgcaacgtca
     6361 cgtcctgccg cggccggatg tcggcgccgc cgatgccgac gatgtcgaag acctcgtcgc
     6421 cgtcgagccc gagcgtctgg gcattggcgc cctccttgaa ctggagcggg agcactccca
     6481 tgccgacgag gttcgagcgg tggatgcgct cgaagctctc ggcgatgacg gcgtggacgc
     6541 ccagcagacg cgtgcccttc gcggcccagt cgcgcgagct gcccgtgccg tactcctgcc
     6601 cggcgatgac gacgagcggg atgccggcgg cctgataatt aaccgaggcg tcgtagatgc
     6661 tcatctgctc gctcacggga tggaggcacg tcacgccgcc ctccgtgccc ggcaccatct
     6721 ggttcttgat gcggacgttg gcgaaggtgc cgcgcagcat cacctgatga ttgccgcgcc
     6781 gcgagccgta ggagttgaag tcttcgacgg agacgccgcg cagcagcagg tagcgaccgg
     6841 ccggcgactt ggccttgatg gcgccggcgg gcgagatgtg gtcggtcgtc accgagtcgc
     6901 cgaagatggc cagcgggcgc gcgccgatga tgtcgcagaa ctcgccgggc tgcatgctga
     6961 acttctcgaa gtagggcggc tcctgaatgt aggtcgattg actctcccac tggtagacct
     7021 cgccggcgac gctcgggatt tcgttccaca tggggttcgc atcggcgaag ctctcataga
     7081 gcatccggta ggtcttcggg ttggtggccg tgctcatgag cgcggagatt tcttcgagcg
     7141 tcggccagat gtctttcagg taaacgtcgc cgtcgccgtt gcttcccttg cccagcggct
     7201 cgctcgttag atcgacgttg atgcgccccg ccagcgcgaa ggcgacgacg agcggcggac
     7261 tcatcaaaaa gttcgcgcga atactttgat ggacgcgcgc ctcgaagttg cgattgcccg
     7321 acagcacgct cgcggcgacg aggtcgttgt cgttgacggc cgcctcgatg cgcgggtcga
     7381 gcgggccgga gttgccgatg caagtcgtgc agccgtagcc gacgagattg aagccgagtt
     7441 cgttgagata cttttgcagc cccgcgcgct caaggtattc ggtgacgacg cgcgagccgg
     7501 gcgcaagcga cgccttcaca ccgggacgca cgcgcaggcc gcgctcgaca gccttcttgg
     7561 cgagcagccc cgccgccagc atcacgctcg ggttcgacgt gttggtgcag gacgtgatcg
     7621 ccgcgatgag cacgtcgccg tggccgagat cgaccgtcgc cgacggaaat tcctcgtgcg
     7681 gaatctccac cagacgatcc ggcgtcggcc ggttgttcat catttccaac tcggtgtgcg
     7741 cgctcgtgtc cttctcgctc accgagccga ggccgccgac cgtctcggtc gccactgtct
     7801 ccggcgcagt ctcctgctcc tgcccgccgc cgccgagaat gggcagtgag cgggtcggct
     7861 cgctgacgcc gatcttgacg cggaagcgcc ggtcgatctc ctccttcgcg cggttgtagc
     7921 cgttctcgcc gatgggcttc tggagcaggt cgaggaactt ctcctttaag tcgccgagtt
     7981 cgatgcgatc ctgcgggcgc ttcggccccg cgacgctcgg cttgatgtca gcgaggtcga
     8041 gctcgtgcag ggtgctgtag tcgcactggc cggcggccgg gatgccgaac atctcctgag
     8101 ccttgaagta gctgcggacg atttcgatct gctcaggcgt gcgccccgtc gccgcgagat
     8161 actcgcacgt cttttcgtcc acggggaaga agcccatcgt cgcgccgtac tcgggagcca
     8221 tgttggcgat ggtcgcgcgg tcggtgaccg gaagcgacga cgccccctcg ccgtgaaact
     8281 cgacgaactt gccgaccacc ttcgcctgcc gcagcaactc ggtgacgcgc agcacgaggt
     8341 cggtggcggt gacgccctcc ttgagcgcgc ccgtcaaatt cacgccgacc acgtccggcg
     8401 tcaggaagta aacgggctgg cccaacatcc cggcctcggc ctcgataccg ccgacgcccc
     8461 agccgacgat gccgaggccg ttgatcatcg tcgtgtgcga gtcggtgccg acgagcgtgt
     8521 ccgggtaata gacgccctct ttctccagca cgccctgcgc gaggtattcg aggttgacct
     8581 gatggacgat gccgatgccc ggcgggacga cgcggaagtt attgaacgcc tgcgtccccc
     8641 actttaaaaa ctggtagcgc gagcggttgc gcttgaactc ggtctccatg ttgagccgca
     8701 gggcgtcggc gctcgaccag aagtccacct gcaccgaatg gtcgatgacc aaatcgacgg
     8761 ggacaagcgg ctcgatgatt ccggggtcgc gccccatgcg ctcgacggcc gagcgcatcg
     8821 ccgccagatc gacgaggagc ggcacgcccg tgaaatcctg caagagcacg cgcgccacgt
     8881 agaaaggaat ctcttcgtcg cgctcggcct gcgcgcccca gttggccagc gcgcgcacgt
     8941 ccgactcttc gaccacatgg ccatcgaagt ttcgcaggat ggattcgagc acgattcgca
     9001 tggagaccgg caggcgcgag acggggccga cgccctcgcg ttcgagctgc gggatcgagt
     9061 agaactgccc ttcgcgtccc cccgcggttt tgaaagtctg gagtgtgttg aagagattgt
     9121 gtgtcatagg agttttcaag aagcctgttt ttgtggtaaa ccttcgagcc gggaacaatg
     9181 gctgcggccg agaccagcgc gagccgaccc ccgactcaca tttgcgccgc ccggactgaa
     9241 acggcgaacc ggtttactgt ctgactgctt tatgtttacg cttcagttta tgttcgcacg
     9301 tcgccggccg tcaagcagac ccttccccga ggagcgtgga cgacgatgac ggacaaaatc
     9361 tcttctgcaa cgccgacgcc catcgccgcc cccggcgcct tcgtccgcac cgtctcaatc
     9421 gacactttcc tcacgggcct tcgctccatc cgcgaatccg agttcaccgt cggccgggtt
     9481 ttcgactacc tcaagcgcca tcccgtcgag gccaacacgc tcagccgcta ccagttcttc
     9541 agccgcaatc actacacgcg caatctcatc ttcaagaatc ccctgtttga actcgtggcc
     9601 gtctgctggg acatagggca gggttcgcag atacacgacc accacgacca gaactgctgg
     9661 atgtcgatgc ccgtggggcg actgcgcgtg cagaactatc acgtcgccga gcgcgacgag
     9721 cgacgctcct acgcgcgcat cgagccgacc gactccttcg acattcaccg cctcgcgccc
     9781 gccgccgagg ttgaccccga ggagccggtg cataaggtct ccaacctgcc cgagttcaaa
     9841 cagcgcgccg tcagcctcca catctactcg cggcccttca acacctgcgt cgtctactcg
     9901 cccgcgaaaa acgactaccg cgtgcaaccg ctgacatatc acagcgaata cgtccggctc
     9961 tgcgagggcg tgcggctctg aaagcggcag ccccgtcaga cgcaaccaac cgccagcccc
    10021 tcacattcgt cgtttgccga acgccacctc cgttgatata gttgccccat ccgaaaatcg
    10081 tcagcaaccc gatagttgat ttcaactttg agatttcaaa tctcaaattc attggggagg
    10141 tggggtcagt gccgaagaag aacccggaag actttacata tctgagcaac gatccgaact
    10201 acgtgcccga tcccgagatc ggcgcgttta tggaagagga gatttcgcgc gcgccgaaag
    10261 actccgacca cctcgcgcaa cgccttcgca acagcacgtc ggcctcgccc agcgacgcgg
    10321 gcggcgatat cgacgcctca tgggaagatg tcaacgacag cggcacggag tccgtctccg
    10381 gcgacaatcc cacgcccgac cagtccgacg tggaggagaa cgcccacgcc atcggcgtca
    10441 acttcgagga caacgaagag ctggaagtca tcgacaagat cgagcgccgc gaccgcgacc
    10501 gcttcgaact cgacccgcgc tcgaagaccg aagacaactc gatataaggc aaaagtaaaa
    10561 aggcaaaagg caaaagtgaa gaggcaatac gcttcctcac ttttgccttt tgccttttta
    10621 cttttgcctt actgaaagct tgctctcggc tggagcgtga tgctcttgac gacgactttc
    10681 ggctcggggt tcgtggtgcc ggcgcgcgtg ggggcgccgc tgatgatgc
//
LOCUS       DASVES010000457         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1056430, whole genome shotgun sequence.
ACCESSION   DASVES010000457 DASVES010000000
VERSION     DASVES010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056430"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1801)
                     /locus_tag="VF240_19010"
     CDS             complement(11..1801)
                     /locus_tag="VF240_19010"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7177356.1"
                     /translation="MSQTQAPLPRPEYPRPQFVRPEWLNLNGEWEFAFDDADRGRELG
                     WHDGRPLEGRITVPFPYQSALSGVGDKHIHEHVWYARTFEAPPRWRERDLLLHFGAVD
                     YRATLWVNGQEVGHNQGGHVPFQFDIAPYLREGANRLALRVEDRQDAHQPRGKQSVSG
                     LPHDIDYYCTTGIWQTVWLEPVPTIRIQEIRITPLAHKNTVELVVYLHAPSAAWRLEA
                     EALDGGRVIARAEERTAVATGWLSLSIPYAKLWSPESPYLYDLRIRLFEGERLLDEVS
                     SYAGLRSIEVRDGRVVLNGEPIYLKMVLDQGYWPDGLLAAPSDEALQSDVGWAKVFGF
                     NGARKHQKIEDPRWLYWCDRLGLLVWGEMPNAREWSSQSEEWLAAEWRRSVRRDYSHP
                     CVIAWVPVNESMGFPGLGLEHPGQYAFIERMVSDTRRLDPTRPIIDNDGWEHTDITDI
                     CAIHDYTPTARELRGRYRLKSEGGDLPPSVWIGDKPLFARGSEYRGQPVVLSEVGGFL
                     TLPPGVPAEERDMLYQFYASADTPDELLAKYRDLLEGIASLKFLAGFCYTQLTDIEQE
                     INGLLTYDRRPKLASENVAEIHRELFGPRP"
     gene            complement(1845..4136)
                     /locus_tag="VF240_19015"
     CDS             complement(1845..4136)
                     /locus_tag="VF240_19015"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX7177357.1"
                     /translation="MPNSQTPDSEPLELWGGVECTVNRVGDQYFDQLERSGHAARHTD
                     LDLFASLGVRALRYPVLWERTAPDGPESADWSWADARLARLRRIGVRPIVGLVHHGSG
                     PRDTSLVDPEFPEKLAGFARAVAVRYPWVDAYTPVNEPLTTARFSGLYGHWYPHGRDT
                     LTFARALLAQCRAVVLSMRAIREVNPRARLVQTDDLGKTFSTRALTYQAEFENERRWL
                     TFDLLCGRVRPGHPMWNYLCGSGVSAAELEWFVANPCPPDIIGVNHYLTSERYLDERL
                     ERYPSCTHGGNGRDSYADVEAVRVLAEGCAGPRALLKEAWERYGLPLAVTEAHLGCTR
                     EEQMRWLKEVWDGARSLRAAGVDVRAVTAWALLGSFDWDSLHTQARGHYEPGVFDLRS
                     PRPRPTALANLLRELSSGARPHHPVLDAPGWWRRLDRLLYLPETRRPSGAPAVEKQWT
                     HKIEGKAQDKVRRLLITGATGTLGRAFARACDARAIPYRLSTRAEMDIADPSSVAAAL
                     DEFEPWAVVNAAGYVRVDDAELEPELCRRENSDGPRVLARACAERGAALVTFSSDLVF
                     DGSTRRPYVESDRPAPLGVYGRSKAAAEASVLAALPSALVVRASAFFGPWDEYNFVTA
                     ALRALAAGERFAAASDAIVSPTYVPDLVSTTLDLLIDGERGIWHLSNRGALSWAELAL
                     RAARLAGLDETLIDARPTASLGLRAPRPLYSALGSERAALLPPLDGALLRYMGECKSA
                     PRGVQKVTPGRRDGSGGFRFAAS"
     gene            complement(4157..>4810)
                     /locus_tag="VF240_19020"
     CDS             complement(4157..>4810)
                     /locus_tag="VF240_19020"
                     /inference="COORDINATES: protein motif:HMM:NF015247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX7177358.1"
                     /translation="FTDSYQAMPLHGYTRMFEKMLDHPNIKVMLNCDYREIEPLIPYR
                     EMIYTGPVDAYFDYRYGKLPYRSLEFKHETHDREVFQQAPVVNYPNEHLYTRVTEFKY
                     LTGQEHAKTSIVYEFPQAEGDPYYPVPRKENAEVYAKYKALADATPDVHFVGRLATYK
                     YYNMDQIVAQALTVYSKIAGSKRALAATARGHYGSNGNGNGNGHRNNSESELALTER"
BASE COUNT          809 a         1559 c         1613 g          829 t
ORIGIN      
        1 tcccaatccg ttacggcctc gggccgaaaa gctcccggtg aatctccgcg acgttttcgg
       61 acgctaactt gggccggcgg tcgtaggtca gaaggccgtt aatctcctgc tcgatgtccg
      121 tcaactgcgt gtagcagaac ccggcgagga acttcagcga ggcgatgccc tctaacaggt
      181 cgcggtactt ggcgagcaac tcatccggcg tatcggccga ggcgtagaat tggtaaagca
      241 tgtcgcgctc ctcggcgggg acgcccggag gaagcgtcag gaagccgccc acctcagaga
      301 gcacgacggg ctggccgcga tactccgagc cgcgcgcgaa aagcggcttg tcgccaatcc
      361 agacgctggg cggcaagtcc ccaccctctg acttcagacg atatcgaccg cgaagctcac
      421 gggccgtcgg cgtgtagtcg tggatggcgc agatgtcggt gatgtctgtg tgctcccagc
      481 cgtcgttgtc gatgatgggc cgggtcgggt ccagacgcct tgtgtcggag accatacgct
      541 cgatgaaggc gtactgtccg gggtgttcca gcccgaggcc gggaaagccc atgctctcgt
      601 tgacgggcac ccacgcgatg acgcaagggt ggctgtagtc gcgtcgcacg gagcgcctcc
      661 actcggccgc gagccattcc tccgactgcg atgaccactc tcgcgcgttc ggcatctcac
      721 cccagacgag cagccccagg cggtcacacc agtagagcca gcgcgggtct tcgattttct
      781 ggtgcttgcg cgcgccgttg aagccgaaca cctttgccca gcccacgtcc gattgaagcg
      841 cctcatccga gggcgcggcc agaagcccgt ccggccagta gccctggtcg agcaccattt
      901 tcagatagat cggctcaccg ttcagcacca cgcggccgtc gcggacttcg atgctgcgta
      961 gtccggcata ggaagagact tcgtcgagca gccgctcgcc ctcgaacagg cgtatccgca
     1021 gatcgtagag gtagggcgac tccggcgacc acagcttcgc gtagggaatg ctcagggaga
     1081 gccagcccgt cgccacggcc gtcctctcct cggcgcgggc gatcacgcgc ccgccgtcga
     1141 gggcctcggc ctccagcctc catgcggccg aaggggcatg gaggtagacc accagttcga
     1201 cggtgttctt gtgggcgagc ggcgtgatcc tgatttcctg gatgcggata gtcggcactg
     1261 gctccagcca gaccgtctgc cagatgccag tcgtacagta gtagtcaatg tcgtgcggca
     1321 gccccgagac ggactgcttg ccgcgcggct gatgcgcgtc ctgccggtct tcgacgcgca
     1381 acgcgagacg gttggcgccc tctcgcaagt aaggcgcgat gtcgaactgg aacgggacgt
     1441 ggccgccctg attgtgtccg acttcctgac cgttgaccca aagagtagcg cggtagtcca
     1501 ctgccccgaa gtggagcagc agatcacgct cacgccaacg gggcggcgcc tcgaaagtgc
     1561 gcgcgtacca gacatgctcg tggatatgct tgtcgccgac gcctgacagt gcgctctggt
     1621 aggggaaggg cacggtgatg cggccttcga gcggtcggcc atcgtgccag ccaagttcgc
     1681 gcccgcggtc ggcgtcgtcg aaggcgaact cccactcgcc gttgaggttt aaccactctg
     1741 gtcggacgaa ctgcgggcgc gggtattcgg gccgggggag gggggcttgc gtctgactca
     1801 ttcttacttt atacgacttt tcagccgacg cctccaactt catctcaact cgcggcaaac
     1861 ctgaatcccc cggaaccgtc ccgccgtccc ggcgtgactt tctgcacgcc gcggggcgcg
     1921 ctcttgcact cgcccatgta gcgcaagagt gcgccgtcga gcggcgggag tagtgcggcg
     1981 cgctcactgc cgagcgcact gtagagcggg cgcggggcgc ggagtccgag cgaggcggtg
     2041 ggccgtgcgt ctatcagagt ttcgtcgaga ccggccaaac gagccgcgcg caatgccaac
     2101 tccgcccacg agagcgcgcc ccggtttgag aggtgccaga tgccgcgttc accgtcgatc
     2161 agcaaatcga gtgtggtgct cacgaggtcg gggacgtagg tcggagaaac gatggcgtcg
     2221 ctcgccgccg cgaagcgctc accagcggcg agcgcgcgca gcgccgcggt cacgaagttg
     2281 tactcgtccc acgggccgaa gaatgcgctc gcgcgcacga cgagggcaga gggcagtgcc
     2341 gcgagcacgc tggcttcggc cgcggcctta ctccgtccat aaacgccaag cggcgcgggc
     2401 ctgtcgctct cgacgtaggg ccttcgggtc gagccgtcga agaccaagtc tgacgagaac
     2461 gtgacgagcg cggccccgcg ctccgcgcac gcccgcgcca ggacgcgggg gccgtcggag
     2521 ttttcacggc ggcacaattc cggctcaagc tcagcgtcat caacgcgcac gtagccggcg
     2581 gcgttaacta ccgcccacgg ctcgaactca tcgagcgccg cggcgactga ggacgggtcg
     2641 gcgatatcca tctccgcgcg tgttgatagt cggtaaggga tggcgcgggc gtcgcaggcg
     2701 cgggcgaagg cgcggcccag cgtgcccgtc gcccccgtga tgagaagtct acgcacttta
     2761 tcctgtgcct tcccttcgat cttatgcgtc cactgctttt caaccgcggg agcgccggac
     2821 ggccgccgcg tctcgggcag gtagagcaga cggtcgaggc gtcgccacca ccccggcgcg
     2881 tcgagaactg gatggtgtgg ccgagcgccg ctcgacaact ctcttaataa gttcgccagg
     2941 gccgtcggac gcgggcgcgg cgagcgcagg tcgaagacgc ccggctcgta gtggcccctg
     3001 gcctgggtat gtaaactgtc ccagtcgaaa gagccgagca acgcccaagc cgtcacggcg
     3061 cgcacgtcca cgcccgccgc gcgcaaactc cgcgcgccgt cccagacctc cttgagccag
     3121 cgcatttgct cctcgcgcgt gcagcccaga tgcgcctcgg tgacggcgag cggcaacccg
     3181 taacgctccc acgcctcttt gagcagggcg cgcgggccgg cgcaaccctc ggcaagcacg
     3241 cgcaccgcct ccacgtcggc gtagctgtcg cgcccgttgc cgccatgcgt gcaactcgga
     3301 taacgctcaa gcctctcgtc gagataacgc tcgctcgtca ggtagtggtt gacgccgata
     3361 atgtcaggcg ggcacgggtt agcgacgaac cattcgagtt ccgccgcgct gacccccgac
     3421 ccgcacaggt agttccacat cgggtgaccg ggccggacgc gcccgcacag cagatcgaag
     3481 gtgagccagc gccgctcgtt ctcgaactcg gcctgatacg tgagggcgcg cgtactgaaa
     3541 gtcttcccga ggtcgtcggt ctgcaccagt cgcgcccgcg ggttgacctc gcgtatcgcg
     3601 cgcattgaga gaacgacggc gcgacactga gcgaggagcg cgcgggcgaa ggtcagggtg
     3661 tcgcgcccgt gcggatacca gtggccgtag aggccgctga agcgagccgt ggtcagcggc
     3721 tcgttgacgg gcgtgtaggc gtccacccac ggatagcgca cggcgacggc gcgcgcgaag
     3781 ccggcgagtt tttcggggaa ctcaggatca acgagactgg tgtcgcgcgg gccgctgccg
     3841 tgatggacga ggccgacaat tgggcggacg cctatgcggc gcaggcgagc gaggcgcgcg
     3901 tctgcccacg accagtcggc gctctccggg ccgtcgggcg ctgtgcgctc ccagaggacg
     3961 gggtagcgca gggcgcggac tccgaggctg gcgaagaggt cgaggtcggt gtggcgcgcg
     4021 gcgtggccgc tacgctcaag ctggtcgaag tactgatctc cgacccgatt gaccgtacac
     4081 tccacgccgc cccacagctc tagcggttca ctatccggcg tttgactgtt cggcatggac
     4141 aaactcccgt ctgtccctac cgctcggtca gagccaactc cgactcggag ttgttgcggt
     4201 ggccgttgcc gttgccgttg ccattgctcc cataatgacc gcgcgccgtc gccgccaggg
     4261 cgcgtttcga gccggcaatc ttcgagtaga ccgtcaatgc ctgcgcgacg atctgatcca
     4321 tattgtaata cttgtaggtg gcgaggcggc cgacgaagtg aacgtcagga gtggcgtcgg
     4381 ccagcgcctt gtatttggcg tagacctccg cattctcttt ccgcgggacg gggtagtagg
     4441 ggtcgccctc cgcctgcggg aactcgtaaa cgatgctcgt cttcgcgtgc tcctgacccg
     4501 tcaggtattt gaactcggtg acgcgcgtgt agaggtgctc gttcgggtag ttcaccacgg
     4561 gcgcctgctg gaagacctcg cggtcgtgcg tctcgtgctt gaactccagc gagcggtagg
     4621 ggagcttgcc gtagcggtag tcgaagtagg cgtccaccgg gccggtgtag atcatctccc
     4681 ggtaggggat gagtggctcg atctcccgat agtcgcagtt gagcatgacc ttgatgttcg
     4741 ggtgatctaa catcttctcg aacatccgcg tgtagccgtg cagcggcatc gcctgatagc
     4801 tgtcggtgaa
//
LOCUS       DASVES010000458        11972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1272825, whole genome shotgun sequence.
ACCESSION   DASVES010000458 DASVES010000000
VERSION     DASVES010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272825"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF240_19025"
     CDS             <1..339
                     /locus_tag="VF240_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7177359.1"
                     /translation="GTHVTEHVLARAISELRKVFDDSAQKPRVIETIPKVGYRLIASV
                     TAATAEENPTFETAANAARPPAARRPTRLEALHTWAGLAALSSATALVLVVIYLLLST
                     RHIGPWHLNH"
     gene            complement(604..1005)
                     /locus_tag="VF240_19030"
     CDS             complement(604..1005)
                     /locus_tag="VF240_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7177360.1"
                     /translation="MRKAIITLALALPAAVSLAACGPTDDAGNTNAGTATNRAAATTI
                     TTPPPATTTKPPVAATDAHATPAADSPRRITVEEARAAFQRGEAVFVDVRGEDAFRQG
                     HIPGALSIPTGTAAQHADKLPKNKLIITYCA"
     gene            1401..2420
                     /locus_tag="VF240_19035"
     CDS             1401..2420
                     /locus_tag="VF240_19035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7177361.1"
                     /translation="MSAGVLLVLCVLLAAAGCMPGSPLETKGTQRTITLYGFSVMKES
                     LEKAIFPAFSAKWKAEHGEQVNFVTSFAGSETITNQILQGVDADVAILSIERDVDRLV
                     QGGFVKQDWRARGSKGIVNKTPFVILVRKGNPKNVRDFADLARPGVRVIHPDPVSSGG
                     AMWSILAMYGSELVKSEAETGGRDGARALQTLQSIWRNVFSTPGSAREARTQFELGHG
                     DALITYELEGMLMREAGAAVEVVVPRSTIFSEHPAVIIDRNVTAEERPAVEAFADYLW
                     SAEAQAAFVKYHFRSVTDESLNAANADFALIELPFTVELFGGWARAYPEIIEGVWRDK
                     AQKKK"
     gene            2468..2830
                     /locus_tag="VF240_19040"
     CDS             2468..2830
                     /locus_tag="VF240_19040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7177362.1"
                     /translation="MRPHEKLDVWGRSVDFVVSVYRATEEFPKEEKFGLTSQIRRAAV
                     SVPANIAEGAGRDSPKEFRHFLSNAQGSASELATELLIARRLGLITEEGYRSVNTELD
                     HIGRMIFGLSQHLKQKDK"
     gene            2990..3751
                     /locus_tag="VF240_19045"
     CDS             2990..3751
                     /locus_tag="VF240_19045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177363.1"
                     /translation="MLLVMLPLVLLPLASIFIFGTSKGLGSFWAALTAPEAVFALKLS
                     IVTSFWATVFNVVFGLFAAYVLSRHTFLGRTAIIVTISLPTAIPTAVAGFALLLLWGP
                     YGLLGQYLSKEFQVMFTPYAIILANIFVTFPLAFGVLMSVFETMDSSLEEASATIGAT
                     RWQTFWHVTLPSLRGALVTGALLTFARAVGEFGSTILVSGNLAARTQTAPLYIFAKFN
                     EGQIEAANAVAVVLALFSFVIFSVLFFARDRLEDK"
     gene            3849..5051
                     /locus_tag="VF240_19050"
     CDS             3849..5051
                     /locus_tag="VF240_19050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177364.1"
                     /translation="MQNDVAAVAASVRGLSKSFKGTRVLEDVSFDVGEGEALVLLGAS
                     GSGKTTILRVIAGLERPDAGRVFLHGRDVTDVPARERSVGVIFQSYALFPRMTVEKNI
                     AYGLALRRRPRAEIKEKVESLLALVKLEEHRRKYPRQLSGGQQQRVAIARTLAYSPEV
                     LLFDEPFGALDAQTRVHLRREIRALLREVNVPAVFITHDQEEALELGDRIAVLNAGRL
                     EQVGTPDEVYNRPETEYVATFLGAANLLLGVASGGRVEIGTASIPAREETARFRDGQA
                     VKLVFRPEDAHLSIGGALPESCRRLTNGRIEEKHFVGAYERLTVRLDLMARRAVEGEP
                     PLYQVTINTEEQRAGLPIFVTRPKPETASTPLKVGDRVAVGLTAFRVLPNYALASERA
                     SRIVDTGK"
     gene            5285..6670
                     /locus_tag="VF240_19055"
     CDS             5285..6670
                     /locus_tag="VF240_19055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7177365.1"
                     /translation="MYFKQFYLGCLAQASYLIGSGGEAVVVDPRRDVDEYIAEAEREG
                     LRIKYVVETHLHADFVSGHRELAARTGAEIVFGEQARATFPHRAVRDGDELRVGAVAL
                     RVMETPGHTPEGISLLVSDTENEDEPRKILTGDTLFIGDVGRPDLSGARGYTTEAMAS
                     MLYDSLHEKLLRLPDSVEVYPAHGAGSMCGRNISKETSSTIGEQRRHNYALQPMPRAE
                     FVRMMTEELPEQPAYFQRDAEINRAGAPALADLPRPRAVSPDELAALAAEGHVVLDVR
                     PAAEFGAGHVPGSLNIGLSGQFASWAGSLIPLGTPVVLVAASEAQAEEAVTRLARVGH
                     ESVAGYLAGGVGAWEQSGRERATLPQISVEELRRKLDEDDDLQVLDVRRAGEYAGGHA
                     PRAVSAALAPDLGERVAKLDPGRPVAVICAGGYRSSAAASLLLPRGFRRLFNVTGGTG
                     AWVAAGFPVEN"
     gene            6769..7161
                     /locus_tag="VF240_19060"
     CDS             6769..7161
                     /locus_tag="VF240_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177366.1"
                     /translation="MHHTFSRRLAILFGVLAPLGETIRRWDTWREWPPNFFDDYLMGA
                     LLLYAAFVSYREPRRGQRYLAAAWGFAIGLGYASFFGHLEELMKVGLNAPDPAPIPHL
                     WVTVIIGVGWLLCILALATTLKRLPDNP"
     gene            7158..8741
                     /locus_tag="VF240_19065"
     CDS             7158..8741
                     /locus_tag="VF240_19065"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7177367.1"
                     /translation="MTRAATSASDELTTLGAAELARRIVGGALSSREIVEAHIRRVEE
                     VNPRVNAVVVRRFEEALREAERVDARRARGETLGPLGGVPFTVKESFDVRGLPTTMGL
                     SARARRTAEEDAPTVARLRAAGAVLLGKTNVPQLVMLNEADNPVYGRTNNPWDAGRAT
                     GGSSGGCAAAVASRCTPLSLGSDIGGSVRLPAHACGVHSLKPTSGRLTMRGHGAIFTG
                     QEAVIAQPGPLARRVEDLRLALDILAATGDETFDPSCPPAPLRDPAEVSVENLRVGFY
                     TDNGVMRPGPAIRRAVEEAARALEARGAAVEEWTPPGIEEAWGVYHRLMFADGTAGIR
                     RDLRGSRRDWRLRQLVLMGLLPRALLRLGALEMSLAGQKHWAWAARHLGRHSVEEYWA
                     LVARRDDYRARFLADWGARGFDAVLCPVEALPAYTHGASVYLSDSLSHTALYNLLGMP
                     AGVVASTRVRAGEESDRVAGLDLCERMARKVERGSTGLPVGVQVAARHWREDVVLAVM
                     SALEAHFRARPDYPVQSEQ"
     gene            8955..10523
                     /locus_tag="VF240_19070"
     CDS             8955..10523
                     /locus_tag="VF240_19070"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HEX7177368.1"
                     /translation="MIVFVATFHGWLAAWLAVRMLFRPHRPVKLLGLTIWPQGMIPRH
                     REKLAQSIGKAVGDELVSQDTVVHALFETGFFQRKVETFVETYAQGLLDTPYPSFVDA
                     LPRAVRAPILDALSALQMRVAEHISEVLKSEETVAALDRFIDSRVDELLARRLGETVG
                     EENFERALGFVEHRFRGVVTERGFERKVRDFVSARLDELADTEATLAEMFTPETVAVV
                     KERIDAQVPPIVEQLTKIATNPRTRTQIGALIKREVDDYYQQLSFFKKIFISRDRVHQ
                     EVDDLVNKTLPRRVGEYLSGEAFEQQAEEFLNSTIDGVLARPVRDIVGQIAPEKLQLI
                     KDQISERILAVARSPELAASVSSYATDALHRVRPHSLRAILEHASPESAERLKSFLSR
                     GLFHILSSDETARTINSILSAQVERLLSAPIGRVSDHVPREKVERAAAALTERITSAA
                     RERLPTAISEFDIGRMVREKVSNYDIKKLEDLVLSVAKQHLRTIELFGLVIGFVIGVV
                     QASMLYFGIIGAGY"
     gene            10748..11746
                     /locus_tag="VF240_19075"
     CDS             10748..11746
                     /locus_tag="VF240_19075"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7177369.1"
                     /translation="MRFTKMVAASAVLLLLLLLAPAGVAAQTTRLRMRSDNVGDWHES
                     ERSDKFTGGRIERGELVPGDPDIWITVNVPSFRLTLWQSGREVKSYFVGVGQKEFPIV
                     IGERRATEVIWNPSWIPPDSAWVRDHPGVRPGQVIRAGSAANPLGKLKIPLGGGYLIH
                     QAKGTGDLGNLVSHGCVRMMKADLYDLAEKIVAARALPVTRRQIERAKLNTKTLQIAL
                     DPPLPVDINYDTAVVEGGVLHLYPDVYSDRRNATIARLRAELRASGVAESALDDETLR
                     EMLSRVTRRKRFAVDVSSIAEGRALSDGRELPLVGQPSTRKAVVRKRKAAVRARSV"
BASE COUNT         2017 a         4060 c         3861 g         2034 t
ORIGIN      
        1 ggcacgcacg tcacggagca cgtgctggcg cgcgccatct ccgagctgcg taaggtcttc
       61 gacgacagcg cgcaaaagcc ccgcgtcatc gagaccatcc cgaaggtcgg ctaccgtctc
      121 atcgcgagcg tcaccgccgc cacggcggaa gaaaatccaa ctttcgagac ggccgccaac
      181 gccgcgcgcc cgccggccgc gcgccgcccg actcgcctcg aagccctgca cacgtgggcc
      241 ggcctcgccg cgctctcctc ggcgacggcc ctcgtgctcg tcgtcatcta cctgctcctc
      301 tcaacccgcc acatcgggcc gtggcacctt aatcactgaa gagagctgtt agcgattagc
      361 tgttagcttt tagccagaag ggagcgccgt cgctcgaaat cgaacgacgg cgctcccttc
      421 tgcgcacggc cgtgagcgga cagctaacag ctaatcgcta acagctacta tctatttcat
      481 cgcttcggtc gggagcttct gactggtcca ggcgtgccag ccgccgagga gagtggcaac
      541 gtcttcgcgg ccgagttgtt gtttgatgtc gagcgccgca cgggcgctgg aggcttcgga
      601 gggtcaagca cagtaggtga tgatgagctt gttcttcggc agcttgtcgg cgtgctgcgc
      661 ggccgtcccc gtggggatgg agagggcgcc ggggatgtgg ccctgacgga aggcgtcctc
      721 gccgcgcaca tcgacgaaga cggcctcgcc tctctggaaa gcggcgcgcg cctcctcaac
      781 cgtgatgcgc cgcggcgagt cggccgccgg cgtcgcgtgg gcgtcggtcg cggcgacggg
      841 cggtttcgtg gtcgtcgcgg gcggcggcgt ggtgatggtg gtggcggcgg cccggttggt
      901 cgccgtgccg gcgtttgtgt tcccggcgtc gtccgtcggc ccgcacgccg cgagggacac
      961 agccgcgggg agcgcgagcg cgagggtgat gatggcttta cgcattaatt aactccgaat
     1021 gttggaatcg tagtcgcggc tgaccggccg gggacaccgc gcgcgccgct ctgtagcatc
     1081 gcacacaggg ggccgcgcgc caaagcgccc gccggcactg aaccggccgc cgccgccgca
     1141 cctttcaacc ccgcggccgg caatcttccc aaaagcgcga aggtgtgtta tagacccacg
     1201 caggcggaat ccaaagagca aaacggacga gtgaggtaag ggctggttaa acgtctctga
     1261 ccccggctga cccttccggg gaaagttgca cccgcgcggg cgtcggagcg cgacgacaac
     1321 gccggcggtt gtcgtcaacg gtcgaaggag gggcgatgag tagtcacgtc aaaacaggac
     1381 ggtcgcgccg cggcccggct atgagcgcgg gcgtcctgct cgtactgtgc gtgctgctcg
     1441 cggcggcggg ctgcatgccg gggtcgcccc ttgagacgaa gggcacgcag cgcaccatca
     1501 cgctctacgg cttctcggtg atgaaggagt cgctggagaa ggccatcttc ccggccttct
     1561 ccgcgaagtg gaaggcggag cacggcgagc aggtcaactt cgtcacctcc ttcgccgggt
     1621 cggagacgat cacgaatcag attttgcagg gcgtcgatgc tgacgtggcc atcctctcca
     1681 tcgagcgcga cgttgaccgt ctcgtgcagg gcggcttcgt caaacaggac tggcgcgcgc
     1741 gcggctcgaa gggcatcgtc aacaagacgc ccttcgtcat cctcgtccgc aaggggaacc
     1801 cgaagaacgt gcgcgacttc gccgacctcg cgcggcccgg cgtccgcgtc atccaccccg
     1861 accccgtcag ttcgggcggc gcgatgtggt cgatactggc catgtacggc tcggaactcg
     1921 tgaagtccga agccgaaacg ggcgggcgcg acggggcgcg cgccctccag accttgcaat
     1981 ccatctggcg caacgtcttc tccacgcccg gctcggcgcg cgaggcccgc acgcagtttg
     2041 agctgggcca cggcgacgcg ctcatcacct acgagctgga agggatgctg atgcgcgagg
     2101 ccggcgcggc cgtcgaggtg gtcgtgccgc gctcgaccat cttcagcgag cacccggccg
     2161 tcatcatcga ccgcaacgtc acggccgagg agcgcccggc cgtcgaagcc ttcgccgact
     2221 acctgtggag cgcggaggcg caggccgcct tcgtcaagta ccacttccgc tccgtcaccg
     2281 acgaatcact caacgccgcc aacgccgact tcgccctcat cgagctgccc ttcaccgtcg
     2341 aactcttcgg cggctgggcg cgcgcctacc ccgaaatcat cgaaggcgtc tggcgcgaca
     2401 aggcgcagaa gaagaagtag tgatgagtga tgagtgataa gtgataagag gagaaaggac
     2461 ggatgttatg agacctcacg agaagctgga cgtatggggc aggtcggtgg acttcgtcgt
     2521 ttccgtttac agggcgactg aagaattccc gaaggaagaa aagttcgggc ttacctcaca
     2581 gatcagacgg gcggccgtct ccgtcccggc gaacatcgcc gagggcgccg ggcgcgattc
     2641 gccgaaagag ttcagacact tcctctcaaa cgcacaaggc tccgcgagtg aactcgccac
     2701 cgagctttta attgcccgcc gcctcggctt gattacggaa gagggctacc gaagtgtcaa
     2761 caccgagctt gaccacatcg gtcgcatgat cttcggcctc tcgcagcatt tgaaacagaa
     2821 ggataagtga taagtgatga gtgataagtg ataagagaaa gcatctgtct tttgtctttc
     2881 ttatcactta tcactcatca cttatcacac gaagcctatg agtagtgcca tttcagccat
     2941 gaggcacgtg cgggggttcg acgcgtcgcg gccattaggg attggggcga tgctgttggt
     3001 catgctgccg ctggtgctgt tgccgctggc ctcgatcttt atcttcggga cgagcaaggg
     3061 gctggggtct ttctgggcgg cgctgacggc gccggaggcg gtctttgccc tcaagctctc
     3121 gattgtgacg agcttctggg ccacggtctt caacgtcgtg ttcgggctgt tcgccgccta
     3181 cgtgctgtcg cgccacacat ttttggggcg caccgccatc atcgtcacca tctctttgcc
     3241 gaccgccatc ccgacggccg tcgccggctt cgcgctgctc ctgttgtggg ggccttacgg
     3301 gctgctcggg cagtatctgt cgaaagagtt tcaggtgatg tttacgccct acgccatcat
     3361 cctcgcgaac atcttcgtca ccttcccgct ggccttcggc gtgctgatgt cggtgttcga
     3421 gacgatggac tcttcgctcg aagaggcgtc ggcgaccatc ggggcgacgc gctggcagac
     3481 cttctggcac gtgacgctgc cgagtttgcg aggcgcgcta gtgacgggcg cgctgttgac
     3541 gtttgcgcgc gcggtcggcg agttcggctc gacgattctg gtgtcgggca acctcgcggc
     3601 gcggacgcag acggccccgc tctacatctt cgccaagttc aacgaggggc agatcgaggc
     3661 ggccaacgcc gtcgccgtcg tcctcgccct gttctccttc gtcatcttca gcgtcctctt
     3721 cttcgcgcgc gaccggctcg aagacaagtg aggcgacaga gggtttgact ttgcggcccg
     3781 gagagtcata cacgaaacgg gaagacgaaa gcgcggtcgc tgatgatggc ggcgcggcgg
     3841 gcggcgctgt gcagaatgat gtggcggcgg tggcggccag cgtgcgcggc ttgagcaaga
     3901 gcttcaaggg cacgcgcgtt ctggaagacg tgagcttcga cgtgggcgag ggcgaggcgc
     3961 tcgtgctgct cggcgcgtcg gggtcgggca agaccaccat cctgagagtc atcgcgggcc
     4021 tggagcggcc cgacgcgggc cgcgtcttcc tccacgggcg cgacgtgacg gacgtgcccg
     4081 cgcgcgagcg cagcgtcggc gtcatcttcc agtcctacgc actgttcccg cgcatgacgg
     4141 tcgagaagaa catcgcctac gggctggcgc tgcgccgccg tccgcgcgcg gagattaaag
     4201 agaaggttga gagcctgctc gcgctcgtca agctcgaaga gcaccgccgg aaatatcctc
     4261 gccagctctc gggcgggcag cagcagcgcg tggcgattgc gcgcacgctc gcctacagcc
     4321 ccgaagtgct cctcttcgac gaacccttcg gcgcgctcga cgcgcagacg cgcgtccacc
     4381 tccgccgcga gatacgcgcg ctgctccgcg aggtcaatgt tcccgccgtc ttcatcacgc
     4441 acgatcagga ggaggcgctg gaactgggcg accgcatcgc cgtcttgaac gccggccggc
     4501 tcgaacaggt gggcacgccc gacgaggtct acaaccgtcc cgagaccgaa tacgtcgcca
     4561 ccttcctcgg cgcggccaac ctcctgctcg gcgtggcgag cggaggccgc gtcgagatcg
     4621 gcacggcctc gatcccggcg cgcgaggaga ccgctcgctt ccgcgacggg caggccgtca
     4681 agctggtctt ccgacccgaa gacgcgcacc tgagcatcgg cggcgcgctg cccgagagct
     4741 gccgccggct cacgaacgga cgtatcgagg agaagcattt cgtcggcgct tatgagcgcc
     4801 tgaccgtgcg gctcgacctc atggcccgcc gcgccgtcga gggcgagccg ccgctctatc
     4861 aagtgaccat caacacggag gagcagcgcg cgggcctgcc catcttcgtc acccgcccca
     4921 agcccgagac ggcctccacc ccgctcaagg tgggcgaccg cgtggccgtc ggactcaccg
     4981 ccttccgcgt gctgcccaat tacgcactgg cctccgagcg cgccagccgc atcgtggaca
     5041 cggggaagta aaacggacag gacggacagg atttgcaggg ctgacaggac gagtcgattt
     5101 gaaatctcaa atctgaaatc gcgtctctga caactgacac ctgacatctc aaatcagcac
     5161 tgtcttcatc ctgtaagtcc tgtcaatcct gtccaaacct cccgccccta cgatcatcgc
     5221 ctgcgttgag gtcgcgcccg aggcaaaata tctataaaat ctccggccga aggagactgc
     5281 cgtaatgtat ttcaaacaat tctacctcgg gtgtctggcg caggcgtcgt acctcatcgg
     5341 ctcaggcgga gaagcggtcg tggtcgatcc gcgccgcgac gtggacgagt acatcgccga
     5401 ggccgagcgc gaaggcttgc gtatcaagta cgtcgtcgag acgcacctgc acgccgactt
     5461 cgtgagcggc caccgcgagc ttgcggcgcg caccggcgcc gagatcgtct tcggcgagca
     5521 ggcccgcgcc accttcccgc accgcgccgt gcgcgatggc gacgagctgc gtgtgggcgc
     5581 cgtcgccctg cgcgtcatgg agacaccggg ccacacaccg gagggcatct ccctgctcgt
     5641 cagcgacacg gaaaacgagg acgagccgcg gaagattctg accggcgaca cgctcttcat
     5701 cggcgacgtg ggacggcccg acctctcggg cgcacgcggc tacacgaccg aggcgatggc
     5761 ctcgatgctc tacgactccc tgcacgaaaa actcctccgc ctccccgact cggtcgaggt
     5821 ctacccggcc cacggcgcgg ggagcatgtg cggccgcaac atctcgaagg agacctcctc
     5881 gaccatcggc gagcagcgcc gtcacaacta cgcgctccag cccatgccgc gcgcggagtt
     5941 cgtgcggatg atgaccgagg agttgcccga gcagcccgcc tacttccagc gcgacgccga
     6001 gatcaaccgc gccggcgccc ccgcgctcgc cgaccttccg cgcccccgcg ccgtctctcc
     6061 cgatgaactc gccgcgctcg ccgccgaagg ccacgtcgtg ttggacgtgc gaccggccgc
     6121 ggagttcggc gcgggccacg tgcccggctc gctcaacatc ggtctctcag gtcagttcgc
     6181 ctcgtgggcc gggagcctca tcccgctggg gacgcccgtc gtgctggtcg ccgcctcgga
     6241 ggcgcaggcg gaggaggccg tgacgcggct tgcgcgcgtc ggccacgagt cggtcgccgg
     6301 atacctcgcg ggcggcgtgg gggcgtggga acagtcaggt cgggaacggg cgacgcttcc
     6361 gcaaatctcc gttgaagagt tgcggcggaa gttggacgaa gacgatgacc tgcaagtctt
     6421 agacgtgcga cgtgcgggcg agtacgccgg cgggcacgcg ccgcgcgccg tgagcgccgc
     6481 gctcgcgcct gacttgggcg agcgcgtcgc gaagctcgac cccggccgcc ccgtagccgt
     6541 catctgcgcc ggcgggtatc gttcgagcgc cgccgcgagc ctgctgctcc cgcgcggctt
     6601 ccgccgcctc ttcaacgtca ccggcggcac cggcgcgtgg gtcgccgccg gcttccccgt
     6661 cgagaactga tgaatgacga gtgataagtg atgagtgatg agtgataaga aaatccggtc
     6721 tgctctttct tatcactcat cactcatcac ttatcacccg cttaccaaat gcaccacacc
     6781 ttcagccgcc ggctcgccat cctctttggc gtgctcgccc cccttggcga gacgatccgt
     6841 cgctgggaca catggcgcga gtggccgccc aactttttcg acgactacct gatgggggcg
     6901 ctgctgctct acgccgcctt cgtctcctat cgcgagccgc gtcgcggcca gcgttacctc
     6961 gccgccgcct ggggcttcgc catcggcctc ggctacgcca gcttcttcgg ccacctcgaa
     7021 gagctgatga aggtcggcct taacgcgccc gaccccgcgc ccatcccgca cctctgggtg
     7081 accgtcatca tcggggtcgg ttggctgctc tgcatcctcg ccctcgccac cacgctcaaa
     7141 cgactccccg acaacccatg acgcgcgccg ccacctccgc gtcggacgag ctaaccacac
     7201 tgggcgcggc cgaactcgcc cgccgcatcg tcgggggcgc gctctcgtca cgcgagattg
     7261 tggaggctca catccggcgc gtggaggagg tgaacccgcg cgtcaacgct gtcgtcgtgc
     7321 gccgcttcga ggaggctctg cgcgaggccg aacgtgtgga cgcgcgtcgc gcgcggggcg
     7381 agacgttagg gccgctcggc ggcgtgccct tcacggtcaa ggagtcgttc gacgttcggg
     7441 gcttgccgac gacaatgggc ctctcggcgc gcgcgcgacg cacggccgaa gaggacgcgc
     7501 ccacggtcgc acgcttgcgc gcggccggcg ccgtcctgct tggcaagacg aacgtcccgc
     7561 agctcgtcat gcttaacgag gcggacaacc ccgtctacgg gcgcacgaat aacccgtggg
     7621 atgcggggcg cgcgacgggc ggctcaagcg gcgggtgcgc ggcggccgtc gcctcccgct
     7681 gcacgccgct gagtctgggc agcgacatcg gcggcagcgt ccgtctcccc gcgcacgcct
     7741 gcggcgttca ctcgctcaag cccacttcgg ggcgactgac catgcgcggc cacggcgcga
     7801 tcttcacggg gcaggaggcg gtcatcgcgc agcccggccc gctcgcgcga cgcgtcgagg
     7861 atttgcgact cgcgctcgac atcctcgccg cgacgggcga tgagactttc gacccgagtt
     7921 gcccgccggc gccgctcaga gacccggccg aagtgtcggt cgaaaacttg cgcgtcggct
     7981 tctacacgga caacggcgtg atgcgccccg gcccggccat ccgccgcgcc gtcgaggagg
     8041 cggcccgcgc actcgaagcg cgcggcgcgg cggtcgagga gtggacgccg cccggtatcg
     8101 aggaggcttg gggcgtctac caccggctga tgttcgcgga cgggacggcc ggcatccgtc
     8161 gcgacctgcg cgggagtcgt cgcgactggc gtctccgtca gctagtcttg atggggctgc
     8221 tgccccgcgc gctccttcgg ctcggcgcgc tggagatgtc tctcgccggg cagaagcact
     8281 gggcatgggc cgcgcgacac ctcggtcggc attcggtgga agagtattgg gcgctcgtcg
     8341 cgcgccggga cgactatcgc gcgcgcttcc tcgccgactg gggcgcgcgc ggcttcgacg
     8401 ccgtcctctg ccccgtcgaa gcgctgcccg catacaccca cggcgcgagc gtctacctct
     8461 ccgactcgct cagccacacc gcgctctaca acctcctcgg catgcccgcg ggcgtcgtcg
     8521 cctcgacgcg cgtccgcgcg ggcgaagaga gcgaccgcgt cgccggcctc gatctgtgcg
     8581 agcgcatggc ccgcaaggtc gagcgcggga gcacgggcct gcccgtcggc gtacaggtgg
     8641 ccgcccgcca ctggcgcgag gacgtggtgc tcgccgtcat gtccgccctc gaagcgcact
     8701 tccgcgcgcg gcccgactac cccgtacaaa gtgagcagtg agcagtgagc agtgagcagt
     8761 taaagaaaaa ctgctctggc gttggttact actcctactg ctcactgctt actgctcact
     8821 cccatttgac gctcgcggcg cgcggcgtta gcatcgcctc atctttttct gaattttcgc
     8881 ggcgcgcttc gagggggacg cacaccatcg aggtactgag ggagatcgtc agcagtaagt
     8941 ggttcgagct gtccatgatc gtcttcgtcg ccaccttcca cgggtggctg gcggcgtggc
     9001 tcgccgtgcg gatgctgttt cggccgcacc gccccgtcaa actccttggc ctcaccatct
     9061 ggccgcaggg gatgatcccg cgtcaccgcg agaagctggc gcaatcaatc ggcaaggccg
     9121 tcggcgacga gctcgtctcg caggacacgg tcgtccacgc gcttttcgag accggcttct
     9181 tccagcgtaa ggtcgagacc ttcgtcgaga cttacgcgca ggggctgctc gacacgccct
     9241 acccatcctt cgtcgatgcg ctcccgcgcg cggtgcgcgc gcccatcctc gacgccctct
     9301 ccgccctcca gatgcgcgtg gccgagcaca tctccgaggt gctcaagagc gaggagacgg
     9361 tcgcggcgct cgaccgcttc atcgatagcc gggtggatga actactcgcg cgccgcctcg
     9421 gcgagaccgt cggtgaagag aacttcgagc gggcgctcgg cttcgtcgag caccgcttcc
     9481 gaggggtcgt caccgagcgc ggcttcgagc gcaaggtgcg cgacttcgtc tcggcccgct
     9541 tggacgagct ggccgacacc gaagcgacgc tggccgagat gttcacgcct gagacggtcg
     9601 cggtcgtcaa ggagcgcatc gacgcgcagg tgccgcccat cgtcgagcag ctcaccaaga
     9661 tcgccaccaa cccacgcacg cgcacgcaga tcggcgccct catcaagcgc gaggtggacg
     9721 actattacca gcagctctcc ttcttcaaga aaatctttat ctcgcgcgac cgcgtccatc
     9781 aggaggttga cgacctcgtc aacaagaccc tgccgcgccg ggtcggcgag tatctttcgg
     9841 gcgaagcctt cgagcaacag gccgaggagt ttttaaactc gaccatcgac ggcgtgctcg
     9901 cccggcccgt ccgcgacatc gtcggacaga tcgcgcccga gaagttgcag ctcatcaagg
     9961 atcaaatctc cgagcgcatc ctcgccgtcg cccgcagccc cgaactcgcg gcctccgtct
    10021 cctcctacgc gaccgacgcc ctgcaccgcg tccgcccgca ctccttgcgc gctatcctcg
    10081 aacacgccag ccccgagtcg gccgaacgcc tcaagagctt cctctcgcgc ggcctcttcc
    10141 acatcctttc gagcgacgag acggcgcgca ccatcaactc gattctctca gcgcaggtcg
    10201 agcggctgct ctcggccccc atcgggcgcg tctccgacca cgtgccgcgc gagaaggtgg
    10261 agcgcgccgc ggccgcgctc accgagcgca tcacctcggc cgcacgcgag cgactgccga
    10321 cggcaatctc cgagttcgac atcggccgga tggtgcgcga gaaggtttca aactacgaca
    10381 tcaagaagct cgaagacctc gtgctctccg tcgccaagca acacctgcga accatcgagc
    10441 tgttcggcct cgtcatcggc ttcgtcatcg gcgtcgtgca ggcgagcatg ctctacttcg
    10501 gaatcatcgg cgccgggtac tgaaataagg atgagggatg agggatgaat aagaattggt
    10561 tcatcggctc attgaatcaa ttcttattca tccctcatcc ctcatcctta gcttgacgcc
    10621 attttgattg caacgcggtt gcgtggtaca accgttccaa cccccctaga cacccaaaca
    10681 ctctcccgag taaaaagtaa acgcgcccga gcggcgcgtc gattagttca aggagaggga
    10741 aacgtctgtg agatttacga aaatggtcgc ggcttcggcc gtcttgctcc tactgctgtt
    10801 gctcgcgccg gccggcgtcg cggcgcagac gacgcgcctg cggatgcgct cggataacgt
    10861 cggcgactgg cacgagtcgg agcgttcgga caagttcacg ggcggccgga tcgagcgcgg
    10921 cgaactcgtg cccggcgacc ccgacatctg gatcacggtc aacgtgccgt cgtttcgtct
    10981 gacgctctgg caatcggggc gcgaggtgaa gtcttacttc gtcggcgtcg ggcagaagga
    11041 gtttcccatc gtcatcgggg agcggcgcgc caccgaggtc atctggaacc cgtcgtggat
    11101 tccgcccgac agcgcgtggg tgcgcgacca ccccggcgtc cggcccggac aggtcatccg
    11161 ggccggcagc gccgccaacc cgctcggcaa gctcaagatt ccgctcggcg gcggctatct
    11221 catccatcag gcgaagggca cgggagacct cggcaacctc gtctcgcacg gctgcgtgcg
    11281 aatgatgaag gccgacctct acgacctcgc cgagaagatc gtcgccgccc gcgcgcttcc
    11341 cgtcacgcgc cgccagatcg agcgcgccaa gctgaacacg aagacgttgc agatcgcgct
    11401 cgacccgccc ctccccgtgg acatcaacta cgacacggcg gtcgtcgagg gcggcgtgct
    11461 ccatctctac cctgacgttt actccgaccg ccgcaacgcc acaatcgcgc gcctgcgcgc
    11521 agaacttcgc gcgagcggcg tcgcagagtc cgcgctcgac gacgagaccc tgcgcgagat
    11581 gctctcgcgc gtcacgcgca ggaagcgctt cgccgttgac gtgagcagca tcgccgaggg
    11641 ccgcgcgctc tccgacggtc gagagttgcc ccttgtcggc cagccgtcca cgcgcaaggc
    11701 cgtggtcaga aagcgtaagg cggccgttcg ggcgcgcagc gtttagaagc ggccgagagg
    11761 cgatggaatg gggcaccgga atagactccc aaatggacgt ctttcgtagc ggcgagtagt
    11821 ccgggataaa agcaaatacg gcgaaatggg cgtctttacc ggcggtttgt agtacgtcta
    11881 ttatgcgtta ggtctctgaa ttgaaggtaa tgtcaaacga aaatccatgt gaggtagttg
    11941 agatttttat agctgaaacc gaatgcacgg tt
//
LOCUS       DASVES010000459         9623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_414443, whole genome shotgun sequence.
ACCESSION   DASVES010000459 DASVES010000000
VERSION     DASVES010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414443"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF240_19080"
     CDS             <1..351
                     /locus_tag="VF240_19080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7177370.1"
                     /translation="PGATFTSLILNIDAAAAGTVNFVVTEDNGQQTMGTFALGASGQN
                     FFTITAINMQRIQSVFFTTDVAMTVTNVDDVAQVRIGGAQTAVPEPTTMLLLGTGLAG
                     IAAKVRRKRRAGSE"
     gene            complement(656..1183)
                     /locus_tag="VF240_19085"
     CDS             complement(656..1183)
                     /locus_tag="VF240_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177371.1"
                     /translation="MALKYVVFFPESDTGRNPLNFETWYITYKPHDGKTWHEGALLKS
                     EIGAGREWLDIQKHGTLTIAIGGAYDAVIHKLLSLQASRAYFTLVVTLQRGPAAKGAP
                     TRIKSEKRSFVALYILNTVEVITLVPKKPGFYFINLRLRGDMKEVLVGEERAGADLFT
                     VNDISEFDAEGRKRW"
     gene            1982..2299
                     /locus_tag="VF240_19090"
     CDS             1982..2299
                     /locus_tag="VF240_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177372.1"
                     /translation="MTTSPRLLVSLSLSLGLGASISAQTPAARTLPAAVTVTAAATDR
                     GVRFTSPSNIVRMQIEVYAEAGPSLFDVSSRGSVFDWALHAGQRLRDSVRHARRAHGD
                     GLS"
     gene            complement(2457..2684)
                     /locus_tag="VF240_19095"
     CDS             complement(2457..2684)
                     /locus_tag="VF240_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177373.1"
                     /translation="MELADATGSIRIASDEIAAIGSGDNTGPALAVALDADSAAIANY
                     TSPVKVAAVYADAAIGTGALARYGIARAFYI"
     gene            2784..3131
                     /locus_tag="VF240_19100"
     CDS             2784..3131
                     /locus_tag="VF240_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177374.1"
                     /translation="MSTKEAGAVERSRRPYDSRVAGVYSTRPGVLGAEKGGATRVGPD
                     EVPVAIVGIVPTKVSAENGPVRVGDLLTTARTSGHAMRCGGRGRCAGASIGKAMEPLR
                     AGRGVIRVLVSLR"
     gene            complement(3495..3944)
                     /locus_tag="VF240_19105"
     CDS             complement(3495..3944)
                     /locus_tag="VF240_19105"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX7177375.1"
                     /translation="MNTKHFLKIAGIKGSSNHAQHRGEMDLLNWWPDDATNKGRLGLD
                     RPGQIANIDAGEITCTKYPDQADAKLLEANASGRHIPEVILTTRREGDDQDLFFALVM
                     KSVVIAGYQLGGSAGDTFPILSITLSFEEATYTSVVQARGAVEGKWK"
     gene            4476..5543
                     /locus_tag="VF240_19110"
     CDS             4476..5543
                     /locus_tag="VF240_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177376.1"
                     /translation="MKRHLFLLPAITLLLLTLPSAPARADDGPRLNKTRLYVHLWQNH
                     SYWPPAATAEQYHTTSWLPSVRFRVFGPVPGGSQFAVDVTRPDGRLWVSLNCPTEEVG
                     AGRRLDVETPRNPPEGDKVYTTGTGVYGFTIRLKNALTGQNQVIYSGKFKVGKVDKSR
                     GNTARKNVVTYYVDHDWTLPMGYMWNEMTQAGNVHLGTSMWFKGASGPKAAYLFYKGK
                     QVASTKESGADISAVPLTTFDNDTGDPRWEMIDFKWYSANFTAPEATVHPSIFFLDKN
                     PGDYEVKVLCGGELCRETKFSIGADGKFVDNGLVAANKIGTPAMLVPVKVVGTHDGQW
                     QPTAWKTDAFYGNPLQGFTAP"
     gene            5583..5690
                     /locus_tag="VF240_19115"
                     /pseudo
     CDS             5583..5690
                     /locus_tag="VF240_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            5985..7085
                     /locus_tag="VF240_19120"
     CDS             5985..7085
                     /locus_tag="VF240_19120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX7177377.1"
                     /translation="MPEILHPQDAIVRVTRSCICGSDLHLYNGSVPDTRVGMTFGHEV
                     TGVVEEVGPEVEKLKIGDHVLVPFNIACGKCVFCQQELYGNCHESNPQATAVGGIFGY
                     SHTGGGYDGGQAEYVRVPYADVSPMVIPEGMDPDDAVMLTDVAPTGYQAAEMGGIQPG
                     DTVVVFGAGPVGIMAARCAWLFGAGRVIVIDHVEYRLEFAKNYAPCEAYNFRSLGDVV
                     VFLKRTTDWLGADVVIDAVGAEAAGRVMQTILGRKLLMQGGSATALHWAINSVKKGGI
                     VSIVGVYGPIDTLVPIGNVVNKGLTLRGNQASVKRLLPRLIEHVQNGILDPKALITHR
                     IPLEEVSDAYRIFSAKLDNCIKPVLLPPSARS"
     gene            7107..7685
                     /locus_tag="VF240_19125"
     CDS             7107..7685
                     /locus_tag="VF240_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177378.1"
                     /translation="MEKTFIDPSQVKGWGVDADPENDPTYPMKKRNNGEHEGYSWERP
                     PQQPVTVEVLHSNERPNVSAVFGTSTPPAGLSGMIRRAAFKYSESSYGHWLPLMLADR
                     VGVVEGYLEDLTRGHVPNVFAERGWKAEWRHNRRSLLTRIAVGAVVASAAVAYLSSGR
                     GDSSAKEQRRRRREALRGASSRKRGRGDIVIT"
     gene            8127..9359
                     /locus_tag="VF240_19130"
     CDS             8127..9359
                     /locus_tag="VF240_19130"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7177379.1"
                     /translation="MYVNEQQILNDDFASSPGAVPNAPDLVCLSHLRWDFVYQRPQHL
                     LSRCAAGRRVFFVEEPVFDSGPLRLEVKEREGGVLVCVPHLPEGLTSEVARDAVQQSL
                     IARLFAERAVRDYVLWYYTPMALSYTRHLNPLATVYDCMDELSAFKGASPEMKACEAE
                     LLERADLVFTGGQSLYEAKRDRHPRVYAFPSSIDAAHFAQARAITDEPADQAAIPHPR
                     LGFFGVIDERFDMELLDGVAEARPDWQLVMIGPVVKVDPESLPRRVNIHYLGMKSYRE
                     LPSYLAGWDVALLLFARNESTRFISPTKTPEYLAAGKPVVSTSIRDVVRPYGEQGLVR
                     IADTAEEFTAAAEAAMSEDAAARTSAADAFLSQTSWDQTWSRMNELIEGVVASRRAYT
                     APAARAAAAAGAVFTAGD"
     gene            9411..>9623
                     /locus_tag="VF240_19135"
     CDS             9411..>9623
                     /locus_tag="VF240_19135"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX7177380.1"
                     /translation="MFDYLIVGAGFAGSVLAERLASSSGKKVLICDKRPHVGGNAYDH
                     YNDAGILVHKYGPHIFHTNSREVFEYL"
BASE COUNT         1776 a         3003 c         3042 g         1802 t
ORIGIN      
        1 cccggcgcga ccttcacgtc gctgatcttg aacatcgacg cggccgccgc cgggacggtc
       61 aatttcgtcg tcaccgagga caacgggcag cagacgatgg gcaccttcgc gctgggcgcg
      121 agcggccaga acttcttcac catcacggcc atcaacatgc agcggatcca aagcgtcttc
      181 ttcacgaccg acgtggcgat gacggtgacg aacgttgacg acgtggcgca ggtgcgcatc
      241 ggcggcgcgc agacagctgt gccggagccg acgacgatgc tgctgctggg gacggggctg
      301 gcgggcatcg ccgccaaggt gcgccgcaag cgtcgggccg gctctgagtg atcaggatta
      361 gttagcagac actaaccctt aaacggcccc cctcgaccgc cccgaccggc ggagggggcc
      421 gtcctctttg gcgtggggcc ggggcttcgt gcggccgcgc gcccggggca tggcgcgcgc
      481 cggcgcgagt gggcggcggg gatcggcaac aatctccgcc gcccttttcg cgtttaaatg
      541 cttcaggttg attttggacg tgtcagcggt cgcgctcggg gcgaatctaa caaagggggc
      601 gtcgtcgtcg tggggacttc tccgccgcct tcttctttac actccgcctc acatctcacc
      661 agcgttttct gccttccgca tcaaactccg aaatatcatt aaccgtaaag agatccgccc
      721 cagcccgctc ctcaccaacc agtacctcct tcatatcccc cctcagcctg aggttgataa
      781 aatagaaccc cggcttcttc ggcaccagcg tgatgacctc gacggtattg aggatgtaga
      841 gggctacgaa gctacgtttc tccgacttga tccgggtcgg cgcgccctta gcggcggggc
      901 ctctttgcag ggtaacgaca agagtaaagt aggcgcgcga agcctgtagt gagagcaact
      961 tgtggatgac ggcgtcgtag gcgcccccga tggcgatggt cagggtgccg tgcttctgga
     1021 tgtccagcca ctcccgcccg gcgccgatct cactcttgag gagcgccccc tcgtgccaag
     1081 tcttcccgtc gtgcggcttg taggtgatgt accatgtctc gaagtttaga ggatttcggc
     1141 cagtatcact ctccgggaag aagacaacgt atttcaaggc catcgcgctt gacctccttc
     1201 aatccgataa gacactgcgg gggaacactt cgccgggttc tgtgttagcg gtcccgtgaa
     1261 cggtttacct taagaggttt gcccggcgct cggttggcgg agcgtatcac aaaatccgca
     1321 ccgaatcgct ctcagttgat tctggacggc cccccgtggc cgtctccggg gcgaatcgcc
     1381 tagcctagcc agcgtggcgc gcaaatggat aggtcacagg gggcgtgagg tcggcgcggc
     1441 gcggcggaaa agttccgcca ttagtccttt cctcaatgcg caacgtcacc gtcaaagggg
     1501 agaggacgtg acgttgcgga gtgggttagt ctcccccaaa gtctaaccca ctccccttcc
     1561 catttcgccc ggcccggtga tagagtgcgg cgcgctcttc ccccgtcgcc gtgcggcaac
     1621 tcgcaccgac agcgaagaga agcgaagggg cggaacgggt cgagaggggc ctcgttccgc
     1681 ccctttctct tttcgctcac atcttcggcc tgtgttgatt ttggacgtgt cacggtcgca
     1741 ccgggggcga atctcttgcc ctgtctgacg tggcgcgacg ctggtgagat cagggtgcgc
     1801 ggccatgtcg agaaatcatt gacaggctgc gcgcccgtcg cgcataactt agtcgctatt
     1861 tcaacctgct ccgccggggg cgtgccaacc gacccgccac cgttcaagcg cccggccacc
     1921 gctgtcgcag cccgcccgac catcgtgatg gcaagcgccc acccattgta aggagtcgca
     1981 catgaccact tcgccgcgcc tactcgtctc tctcagcctg tctctcggcc tcggcgcctc
     2041 gatcagcgcc cagacgcccg ccgcccgcac tttgcccgcg gccgtcacgg tgacggccgc
     2101 ggccaccgac cggggcgtgc gcttcactag cccgagcaac atcgtgcgga tgcagataga
     2161 ggtctacgcg gaggcggggc cgtccctctt cgacgtcagt agtcggggca gcgtcttcga
     2221 ctgggcgctt cacgccgggc aacggctacg agattcagtt cggcacgccc ggcgggcaca
     2281 cggggatggc cttagttaaa ccctccgggc ccgccggcag ggccgacgtc cggtttgacg
     2341 gctcgaactt gagtctgctg gccggcacgg gcaccaccgc tccgtgctgc ggggtcgtcc
     2401 tcaacacggc gggcgacgtc gtcatcgggg ttaattcttt ttctggcgct aacaggctat
     2461 atgtagaagg cccgagcaat gccatatcgg gccaaagcac cagtgccgat ggcggcgtcg
     2521 gcgtatacgg cagcaacttt tacgggagag gtgtagttgg cgatggcggc actatcggcg
     2581 tccagggcta cagccaaagc gggaccggta ttgtcgccag aaccgatagc ggcaatttcg
     2641 tcgctggcta tgcgaatgct gccggtggcg tccgcaagtt ccacatcaac atcaacggca
     2701 cgtatgtcac cgggtctgac ttcgccgagt cgctgccggc acgcggcggc aagtccgcct
     2761 acgaagccgg cgacgtcctc gtcatgagta caaaggaggc cggcgccgtc gagcgcagcc
     2821 gccgccccta cgactcgcgc gtggccggcg tctactcgac ccgccccggc gtgctcggcg
     2881 ccgagaaggg cggcgcgacc cgcgtcggcc ccgacgaggt gccggtggcc atcgtcggca
     2941 tcgtcccgac caaagtctcg gcggagaacg gcccggtccg cgtcggcgac ctgctgacga
     3001 cggcgcgcac gagcggccac gccatgcggt gcggcgggcg ggggaggtgt gcgggcgcga
     3061 gcatcggcaa ggccatggag ccgctgcggg cggggcgcgg cgtgatcagg gtgctggtga
     3121 gcctgcgcta gccggccgtc gcgccgcagt gtgccgggag cgaacgcaag ggacgcgcgg
     3181 cgggtctcgt tctactcacc ggcgcacctt ctttcggttc ggcgtctgcc tctcccggtg
     3241 accgcaaggc ggcctcaacc tatagtcgag aagagggggc ggctgacacg ctcgcccccg
     3301 gttcatccgc acttttgatg acgctaaatt cggtaagctt ggaagtgggc gacggctaaa
     3361 cgaaatttct actcaacctg actaatgccc gtgtcgccac tggtggcgaa catcgacaga
     3421 cagcgccttt acgaacactt cacttcaaac gaactgagcg ccggccgccg ccgttacggt
     3481 ttggacctct tcttttattt ccacttcccc tccaccgcgc ctcgcgcctg gaccaccgag
     3541 gtgtatgtgg cttcctcgaa gctgagagtg atggataata tcggaaatgt gtcgccggcc
     3601 gaaccgccga gctgatagcc ggcgatcaca acagacttca tcacgagcgc gaagaagagg
     3661 tcctgatcgt caccctcgcg gcgagtggtg agaatgactt cggggatgtg tcggcccgag
     3721 gcgtttgcct ccaggagttt ggcatccgct tggtcggggt attttgtgca ggtgatctcg
     3781 ccggcgtcta tatttgctat ttgaccgggg cggtccaacc caagcctccc tttgttcgtt
     3841 gcatcatcgg gccaccaatt gagcaggtcc atttcgccgc gatgttgagc gtgattgctg
     3901 ctgcctttga tgccggcgat tttgaggaag tgctttgtgt tcataacaaa catctccttg
     3961 cgggaggggc accgctaatg gcaaacctga gcgagttgga atgtttggtg ggatggaaag
     4021 cctgtcgggc gcgggccacg atatgccaac tacggtggcg gcggagatgt acgtcttcag
     4081 gtggtgatcg ccaatctaca gaattagaat catcggaatt atccgagata ccgacaacgt
     4141 atttcaaatc cactgcttca cctccttcaa tccgataaga cactacgggg aaaacatatc
     4201 accggggcct gtgttagcga ccccgtgaac ggtttgcctc aagaggattg cccggcgctc
     4261 ggttggtgga gcctatcaca aacttcgcac agaatcagtg gcaacgtagc ataggcgcga
     4321 ggggcggcgg cgaaggctgt acaacggggt tcgtgcggag tacgctggcc gcgaatcgag
     4381 tcccggctca actcccttct cggctcctct ggaatcctcc agattgcctg aagccttcag
     4441 ctcagctatt ccgaaactta aaaggagttc tacccgtgaa gcgccaccta ttcttactgc
     4501 cggctattac tcttcttcta ctcacgcttc catcggcccc ggcccgcgcc gacgacggcc
     4561 cccggctcaa taagacgaga ctctatgttc atctctggca gaaccacagc tactggcctc
     4621 ccgcagcgac ggcggagcaa taccacacca cgtcgtggct gccgagcgtg cggttcaggg
     4681 tcttcgggcc ggtgccgggc ggcagccagt tcgcggtcga tgtcacccgg cccgacggcc
     4741 gtttgtgggt gagcctcaac tgcccgaccg aagaggtcgg ggcgggtcgg cggctcgacg
     4801 tggagacgcc gcgcaacccg cccgagggcg acaaggtgta cacgacgggc acgggcgttt
     4861 acggtttcac catccgcctg aagaatgcgc tgacggggca gaatcaggtc atctactccg
     4921 gcaagttcaa ggtcggcaaa gttgataaga gccgggggaa cacggcgcgc aagaacgtcg
     4981 tcacctacta cgtcgatcac gactggacgc tgcccatggg ttacatgtgg aacgagatga
     5041 cgcaggccgg caacgtgcat ctcggcactt cgatgtggtt caaaggcgcg tccggcccga
     5101 aggcggccta cctcttctac aagggcaagc aggtggcgag cacgaaagag tcaggcgccg
     5161 acatctccgc tgtgccgttg accacttttg acaacgacac cggagacccg cgttgggaaa
     5221 tgatcgactt caaatggtac tcggcgaact tcaccgcccc ggaagcgacg gtgcatccgt
     5281 ctatcttctt cctcgacaag aaccccggcg actacgaggt caaggtgctg tgcgggggcg
     5341 agttgtgccg ggagacgaaa ttcagcatcg gggccgatgg gaagttcgtg gacaacggcc
     5401 tcgtcgcggc caacaagatc ggcacaccgg ccatgctggt tccggtgaag gtcgtcggca
     5461 cgcacgacgg gcagtggcag ccgacggcct ggaagaccga tgctttctac ggcaaccctt
     5521 tacaggggtt cacggcgccc tagagcctgt gatgaacttt tgagtgtggt aagttttggc
     5581 gcatgcgacg ccaaaactat cccagtgacg tgactgatga ggagtgggcg ctcgtcgccc
     5641 cctacctcgc cctgatgact gaggacgccc cgcaacgctc tcaccccttg ccagcgcaac
     5701 tatatcgctg atactccaca gcgtcttaga cacgccccgc cgtgggcgcg tgtgcgtgag
     5761 cgtacagccc gccgctcgtt tcttcgctag aatctacctt cccccttcat cccctccaaa
     5821 gagccatcaa ggtgtggcgg agccttggtc tccgggtcgg gagctggcga tcttgagggt
     5881 cggagattaa gttaagcggc agacgttaaa aaaggaaagg aacaagttct atgctggcga
     5941 tgaatttccg ggggcctctg cgcgtccgta tcgaccataa acccatgccg gaaatcctgc
     6001 atccacagga cgccatcgtc cgggtcacgc gctcttgtat ctgcggctcc gatctgcatt
     6061 tatacaacgg gtccgtgccg gacacccgcg tggggatgac gttcggccac gaggtcacag
     6121 gtgtcgtgga ggaggtcggc ccggaggtgg agaagctgaa aatcggcgac cacgttctgg
     6181 tgcctttcaa catcgcctgc gggaagtgtg tcttctgcca gcaggagctt tacgggaatt
     6241 gccacgagtc gaacccgcag gccaccgcgg tcggcggcat cttcggctat tcgcacaccg
     6301 gcggcggcta cgacggagga caggccgaat acgtccgcgt cccttacgcg gacgtgagtc
     6361 ccatggtcat cccggaaggc atggacccgg acgacgcggt gatgctcacc gacgtggcgc
     6421 cgaccgggta tcaggccgcc gagatgggcg gcattcaacc cggcgacacc gttgtcgttt
     6481 tcggggccgg ccccgtgggc atcatggcgg ccagatgcgc gtggctcttt ggggcggggc
     6541 gcgtcatcgt gattgaccac gtggagtatc gtctggagtt cgcgaagaac tacgcgccct
     6601 gcgaggcata taacttcagg tcgctgggag acgtggtggt cttcctcaag cgcaccaccg
     6661 actggctggg ggccgacgtg gtgatagacg ccgtgggggc cgaagccgcg ggccgtgtga
     6721 tgcagacgat cctcggcaga aaattattga tgcagggcgg ctccgccacc gcgctgcact
     6781 gggcgatcaa ctccgtcaag aagggcggca tcgtctccat cgtcggcgtg tacggcccga
     6841 tcgacaccct cgtcccaatt gggaacgtgg tgaacaaggg cttgaccctg cgcgggaacc
     6901 aggcgtcggt gaagcgtctg ttgccgcgac tgatcgagca cgtgcagaac ggcatcctcg
     6961 atccgaaagc gctgatcaca caccgcatcc ctctggagga agtgtcggac gcgtaccgga
     7021 ttttttcggc gaagctcgat aactgcatca agccggtgct gctgccgcct tcggccagaa
     7081 gctaggaagg aaccgtcatg gagagtatgg aaaagacatt catagacccc tcgcaggtca
     7141 aggggtgggg cgtggacgcc gacccggaaa acgacccgac ctatccgatg aagaagcgca
     7201 acaacggcga acacgagggc tacagttggg agcgcccgcc tcaacagccg gtcactgtcg
     7261 aggtgctgca ttccaacgag cggccgaacg tctcggccgt gttcgggact tcgacgccgc
     7321 ccgcgggact gagcggcatg atccgccgcg ccgctttcaa atacagcgag tccagctacg
     7381 gacactggct gccgctgatg ctggccgacc gtgtgggcgt ggtggaaggc tacctggaag
     7441 acctcacgcg cggccacgtc cccaacgtct tcgcggagcg gggctggaag gcggagtgga
     7501 gacacaacag aagaagtctg ttgacgcgca tcgccgtcgg ggcggtggtg gcttccgccg
     7561 ccgtcgccta cctgagtagc gggaggggcg attcttcggc gaaggagcaa cgacgccgtc
     7621 gcagggaagc tctgcgcgga gcttcgtccc gtaaacgggg aaggggtgac atcgttatca
     7681 cctgacacgg tttctgcatc tgaccacggc ggtaaattcg cattcctctt cttgcgcctt
     7741 cccgaaggcc cgggcaggtg agctgtgtcg ttctactact gcacgcggtt tggatacgtg
     7801 atggcagcgg tactccggtc gccaggccgc ggcgatcatg ctgtacctgt cagtgcccgt
     7861 tgtttgacga ctgcgcgaac actccggtaa gcccctcgaa ggcgctcagt acccacacgt
     7921 acagacaaat tcctcaatct ccagtaccct aaaagacccc ttaagtctga acaaatcgga
     7981 tgggcgcgtc cgtcgcatga gagcttcgga acgcttcctg accgcagcct gtaatccccc
     8041 gcacagcctg cactttttcg gcggactcgc cgcttttttt aactgagtcc gaggaatatt
     8101 ctgacgccgc ggcatcggag acaggtatgt acgttaacga acagcaaatc ttaaatgacg
     8161 atttcgcgtc ttcgccgggg gccgtgccga acgcgcccga cctcgtctgc ctttcacacc
     8221 tgcgctggga ttttgtttac cagcggccac aacacctcct gagtcgctgc gccgcggggc
     8281 gccgggtctt ctttgtcgag gagccggtct tcgacagcgg gccgctccgc cttgaggtga
     8341 aggagcgcga aggcggcgtg ttggtgtgcg tgcctcattt gcccgagggg ctgacgagcg
     8401 aggtcgcgcg cgacgccgtg cagcaaagct tgatcgcacg cctgttcgcc gagcgcgccg
     8461 tgcgcgacta cgtcctgtgg tactacacgc cgatggcgct ctcctacacg cgccacctga
     8521 acccgctcgc gaccgtctac gactgcatgg acgagctgtc ggccttcaag ggcgcatccc
     8581 ccgagatgaa ggcgtgcgag gcggagctgc ttgagcgcgc cgacctggtc ttcacgggcg
     8641 ggcagagtct ctacgaggcc aagcgcgacc gccacccgcg cgtctacgcc ttcccgtcga
     8701 gcatcgacgc cgcccacttc gcgcaggccc gcgccatcac cgacgagccg gcggatcagg
     8761 cggccatccc acacccgcgt ctgggcttct tcggcgtcat cgacgagcgg ttcgacatgg
     8821 aacttctcga cggcgtcgcc gaggcccgac ccgactggca actggtgatg atcgggccgg
     8881 tagtcaaggt tgaccccgag agcctgccgc gccgcgtcaa catccactac ctcggcatga
     8941 agtcgtacag ggaactcccg tcgtacctcg cgggctggga cgtggcgctg ttgctgttcg
     9001 cacgcaacga gtccacgcgc ttcatcagcc cgaccaagac gccggaatac ctcgcggccg
     9061 ggaagccagt cgtctccacc tcgatccgcg atgtcgtgcg cccttacggg gagcaggggt
     9121 tggtgcgcat cgccgacacg gccgaagagt tcacggccgc cgccgaggcc gcgatgagtg
     9181 aggacgcggc ggcgcgcacg tccgccgcgg acgccttcct gtcgcagact tcgtgggatc
     9241 agacgtggtc gcggatgaac gaactgatcg agggagtcgt ggcgtcgcgg cgcgcgtata
     9301 cggcgccggc ggcgcgcgcg gccgccgccg ccggcgccgt atttacggcg ggcgattgaa
     9361 attttcattt aacggttaga aataattacg aaccaacaag ggggggagaa atgttcgatt
     9421 atttgatcgt cggggcgggc ttcgccggga gcgtgttggc cgaacgcctg gcctccagct
     9481 caggtaagaa ggtcttaatc tgcgacaagc gcccgcacgt cggcggcaac gcctacgacc
     9541 actacaacga cgcggggatt ttagtccaca agtacggccc gcacatcttc cataccaact
     9601 cgcgcgaggt gttcgagtat ctc
//
LOCUS       DASVES010000460         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1649593, whole genome shotgun sequence.
ACCESSION   DASVES010000460 DASVES010000000
VERSION     DASVES010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649593"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2084)
                     /locus_tag="VF240_19140"
     CDS             complement(<1..2084)
                     /locus_tag="VF240_19140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177381.1"
                     /translation="MDEQLLREFLAGAEDHFEALFADIKLLRARRDDGRARRELTARV
                     FRHVHTIKGTASAAGLAGVGRLAHEFETLLEAVRGRRVAADDDVLDASEDAVEAMSSL
                     IAGAAHARSDEPAQELVERLRLLASAGGEDVNASAFATRADALDELPAELAVSLGEDE
                     ARRLREAVGEGARAYALTVDFDLADFDERFRELSRALSETGEVVATLPGASARAAAVN
                     FRIVYASNAPREEIERLVAPFGVTLIDGRDATEGAATGEGFRPGVFEETEGVASVPAG
                     EFDEVAGAEEALASSPSPALSRLTMLVRVPLGVLDELISDTHELFAAVSGALEHALES
                     LPEGDATRAELETRAPLVRRGFVDLEERLTGLRMAPVRQTLERAARVGASTARAAGRE
                     VEFETAGGDVCLDRPLAEAVTEALLHLVRNAVHHGVETAEERRAAGKPARGRVRLEAV
                     AEGARVLLRVTDDGRGVDVESVARAARRRGVIEAGASLTERQALRLIFRPGFSTAAQV
                     SDASGRGVGLDVVERMVEAAGGEMRVWSRAGAGATFELRLPTTLALVPTVVVRACGRA
                     YCLDAGHVAGAGAARLSDARDERGRTLVDWDGDALPFVSLGELLGADDDHHTATVAGG
                     QVRVILSSAREGAEAEARPRRVAVGVEDFEERGEVLVRWLGRHATRWRGVSGAAELAD
                     GTVALLLDLPRLL"
     gene            complement(2220..2861)
                     /gene="nusB"
                     /locus_tag="VF240_19145"
     CDS             complement(2220..2861)
                     /gene="nusB"
                     /locus_tag="VF240_19145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7177382.1"
                     /translation="MGSRRKARECALQMLFAADVVGAAKEAGERPRADELVRVYWDEL
                     ADPEVDEAGREFATRLVVGALARRDELDERIRSRAEHWRISRMAVVDRNILRLAVYEF
                     LYELTPRTVAINEALEIARRFSTYEATQFINGILDAIKRDLDRERPQDDDKLDRSEEA
                     IPEVETASDAEADSSSNSTAEEEEAPQAEASDTAGEASAESDGEQVKAVNSES"
     gene            complement(2945..3427)
                     /gene="ribH"
                     /locus_tag="VF240_19150"
     CDS             complement(2945..3427)
                     /gene="ribH"
                     /locus_tag="VF240_19150"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7177383.1"
                     /translation="MQPVTHQGRLNAEGFRFAIISSRWNDFLTARLVEGALDALARLG
                     AAEDSVEHFRVPGSFEIPLLAMRVAESERFDAVICLGTIIRGETPHFEYVAGEVTKGI
                     AHAAVETGVPVLYGIVTADTLEQAIDRAGVKAGNKGFEAALAAVELVNLYKDVFKSDE
                     "
     gene            complement(3580..>4192)
                     /locus_tag="VF240_19155"
     CDS             complement(3580..>4192)
                     /locus_tag="VF240_19155"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7177384.1"
                     /translation="QDFCAGTDIDELKALDEAGARATAERGQRVCNSVELCRIPVIAA
                     VQGQAAGGGCELALSCHLRVAAADARFSLPELRLGVMPGYGGTQRLARAAGQGRALGA
                     MLAGDGLGADEALRRGLVNGVAAAAGDVVNEAESLARRITDTAAPLAVRACLEAVTRG
                     ARLPLEDGLALEAELFARLFSTEDVREGTRAFLEKRKPSFRGQ"
BASE COUNT          658 a         1525 c         1371 g          638 t
ORIGIN      
        1 agcagccgcg gcaggtcgag caacaaggca accgtcccgt ccgccaactc ggccgcgccg
       61 ctcacgccgc gccagcgcgt cgcgtgccgc ccgagccaac gcacgagcac ctcgccgcgc
      121 tcctcgaaat cttccacgcc caccgccacg cggcgaggcc gcgcctccgc ttccgcgccc
      181 tcgcgcgcgc ttgagaggat cacgcgcact tgtcctccgg ccacggtggc ggtgtggtgg
      241 tcgtcatcag cgcccaacaa ctcgccgagc gacacgaagg gcagcgcgtc gccatcccaa
      301 tcgacgagcg tgcggccccg ttcgtcgcgc gcgtcggaga gtcgcgccgc gccggcgccc
      361 gcgacgtgac cggcgtcgag acagtaggcc cgtccgcagg cgcgcacgac caccgtgggg
      421 acgagcgcga gcgtcgtcgg cagtcgcagc tcgaaggtcg cgccggcccc cgcgcggctc
      481 cacacgcgca tctcgccgcc ggccgcctcc accatgcgct ccaccacgtc gaggccgacg
      541 ccgcgccccg aggcgtcgga aacttgcgcg gcggtcgaga agccggggcg gaagatgaga
      601 cgcagcgcct gccgctccgt gagcgacgcg cccgcttcga taacgccgcg ccgccgcgcc
      661 gcgcgcgcca cgctctcgac atcgacgccg cgcccatcgt ccgtgacgcg aagcagaaca
      721 cgcgccccct cggcgacggc ctccagtcgg acgcgccccc gcgccggctt gcccgccgcg
      781 cgccgctcct ccgcggtctc gacgccgtga tgcacggcgt tacgcacgag atgcagcagc
      841 gcttcggtga ccgcctcggc gagcggtctg tcgaggcaaa cgtcgccgcc tgccgtctcg
      901 aactcgactt cgcgaccagc ggcgcgcgcc gtgcttgctc cgacgcgcgc cgcgcgctca
      961 agcgtctgcc gcaccggcgc catccgcagc cccgtcagcc gctcctccaa atcgacgaag
     1021 ccccggcgga cgagcggcgc gcgcgtctca agctccgcgc gcgttgcgtc gccctccggc
     1081 aaactttcga gcgcgtgttc gagcgcgccc gacactgccg cgaacaactc atgcgtgtcc
     1141 gagatcaact cgtcgagcac tccgagcggc acgcgcacga gcatcgtcag ccgcgagagc
     1201 gccggcgacg gagaagacgc caacgcctct tctgcgcctg ccacttcatc aaactcccct
     1261 gccggcacgg aggctacgcc ctccgtctct tcaaacacgc ccggcctgaa gccctcgccg
     1321 gtggccgcgc cctccgtcgc gtctcgtcca tcgatgagcg tgacgccgaa tggcgcgacg
     1381 agccgttcga tctcttcacg cggcgcgttg ctcgcgtaga cgatgcgaaa gttgacggcg
     1441 gcggcgcgcg ccgatgcgcc gggaagtgtc gcgacgacct cgccagtctc ggagagagca
     1501 cggcttagtt cgcggaaacg ttcgtcgaaa tccgcgagat cgaaatcgac cgtcagggca
     1561 taggcgcgcg cgccctcgcc gacggcctcg cgcagacgcc gcgcctcgtc ttcgcccagc
     1621 gagacggcca gctcggcggg cagttcgtcg agcgcgtcgg cgcgtgtggc aaacgcgctc
     1681 gcgtttacgt cctcgccgcc ggcagacgcg agcaagcgca ggcgctcgac gagttcctgc
     1741 gccggctcat cagagcgcgc gtgggcggcg ccggcgatga gcgaggacat ggcctcgaca
     1801 gcgtcctcgg atgcgtcgag cacatcgtcg tccgcggcga cgcgacggcc gcgcaccgct
     1861 tcgagcagcg tctcgaactc gtgcgcgagg cggccgaccc ccgccagccc ggcggccgag
     1921 gccgtcccct tgatggtgtg gacgtggcgg aacacgcgcg cggtcaactc ccgccgggcg
     1981 cgcccgtcgt cgcgccgcgc gcgcaggagc ttgatgtcgg cgaacagggc ctcgaagtgg
     2041 tcttcggccc cggccaggaa ctcgcgcagc agttgctcat ccataatgtg gaatcgtaaa
     2101 tcgtaaatcg taaatcgtaa atcgaaagtc gtgagtcgcg cctcccttaa acgccgacgg
     2161 cccgcgccgg ggtggcagcg ggccgcgtga gtctcgcacg acgatttacg atttacgatt
     2221 cacgattcac tgttaacggc cttcacctgc tcgccgtcgg actcggcgga cgcttctccc
     2281 gcagtgtcgc ttgcttcagc ttgtggcgct tcttcttctt cggcggtgct gttgctgctg
     2341 ctgtcggctt cggcatccga cgcggtctca acttcgggga tggcttcctc ggagcggtcg
     2401 agcttgtcgt cgtcctgcgg gcgctcgcgg tcgaggtcgc gcttgatggc gtcgaggatg
     2461 ccgttgataa actgcgtggc ctcgtaggtg gagaagcggc gggcgatctc cagggcctcg
     2521 ttgatggcga cggtgcgggg cgtcagctcg tagaggaatt catagacggc gagacgcagg
     2581 atgttgcggt caacgacggc catgcgcgag atgcgccagt gctcggcgcg cgaacggatc
     2641 cgctcgtcca actcgtcgcg gcgcgcgagc gcccccacga cgagccgcgt ggcgaactcg
     2701 cgccccgcct cgtccacctc ggggtcggcc agctcgtccc agtagacgcg caccaactcg
     2761 tcggcgcgcg gccgctcgcc ggcctctttg gccgccccca ccacgtctgc cgcaaagagc
     2821 atctgcaagg cgcactcacg cgccttacgc ctcgaaccca taaacacccc agtgaaatag
     2881 tgataagtga tgagtgataa gtgataagaa agagcaagcc gattttctta tcacttatca
     2941 ctcatcactc atcactttta aaaacgtcct tgtagagatt gaccaattcg acggccgcca
     3001 acgcggcctc gaagcccttg ttgccggcct tgacccccgc gcggtcgatg gcctgctcca
     3061 gcgtgtcggc ggtgacgatg ccgtagagga cgggcactcc cgtctccacg gccgcgtgcg
     3121 cgatgccctt cgtgacctcg cccgccacgt actcgaagtg cggcgtctcg ccgcgaatga
     3181 tcgtccccag acagatgacc gcgtcgaacc gctccgactc ggcgaccctc atcgccaaga
     3241 gcggaatctc gaacgacccc ggcacgcgga agtgctcgac cgagtcttcg gccgcgccga
     3301 ggcgcgcgag cgcgtcgagt gcgccctcga ccagccgggc cgtcaggaag tcgttccagc
     3361 ggctcgaaat gatcgcgaag cgaaacccct cggcgttcaa gcgcccctga tgcgtgacag
     3421 gctgcaaagt tttacgcggc gtctccaagt agtttaggaa ttgtgggcaa gggcagtata
     3481 ggaaaggctg tgacgggtga caagtgtaga gcagtgagcg gtaagcagtg agcagttaat
     3541 aaaactttct tccactgctc actgcttacc gctcaccgct cactgccctc tgaaagaggg
     3601 cttgcgcttt tcgaggaagg cgcgcgtgcc ttcgcgcacg tcctcggtgg agaagaggcg
     3661 ggcgaagagt tcggcttcga gggcgaggcc gtcttcgagc ggcaggcggg cgccgcgcgt
     3721 gacggcttcg aggcaggcgc gcacggcgag cggcgcggcc gtgtcggtga tccggcgcgc
     3781 gagactttcg gcctcgttaa cgacatcacc cgcggcggcg gcgacgccgt tgacgagtcc
     3841 gcgccgcagc gcctcgtccg ccccgagtcc gtcgcccgcg agcatcgcgc cgagcgcgcg
     3901 gccctgcccc gcggcgcgcg cgagccgctg cgtgccgccg tagccgggca tgacgccgag
     3961 ccgcagttcc ggcagactga aacgcgcgtc cgccgcggcg accctcaggt ggcacgagag
     4021 cgccagctcg cacccgccgc ccgccgcctg cccctgcacg gcggcgatga cggggatgcg
     4081 gcacaactcc acgctgttgc acactctctg cccccgctcg gccgtggcgc gcgcgcccgc
     4141 ctcgtcgagc gccttgagtt catcgatgtc cgtgccggcg cagaagtctt gg
//
LOCUS       DASVES010000461         7711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_669012, whole genome shotgun sequence.
ACCESSION   DASVES010000461 DASVES010000000
VERSION     DASVES010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669012"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VF240_19160"
     CDS             <1..277
                     /locus_tag="VF240_19160"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177385.1"
                     /translation="FRQEILIRISSYARKGRQFEYNSHERLKEAIEKKIFSDLKDVVK
                     ITTSVKTPDPDQLRRINEVVDRLVKEHGYCPVCANELLTYVGTLLSR"
     gene            377..1564
                     /gene="yhbH"
                     /locus_tag="VF240_19165"
     CDS             377..1564
                     /gene="yhbH"
                     /locus_tag="VF240_19165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HEX7177386.1"
                     /translation="MTEDFISGTIQRNDWSLQRKGVIDQERHRERVKEAVRQNLGSIV
                     SNEAIILSDGRRTVKVPIRSLDEYKFRFDHRKRKHVGQGDGKTRVGDVIAREGQPGQG
                     PGQGSGPGGQGAGAEYYEAEVNIDELAAMIFEDLRLPFLEEKAKKAVQARTTRFNEIR
                     RTGVMSNLDKRRMILENIKRNAREDGRPSFKGVRKEDLRFKSWEEENRYESNAVVLAL
                     MDVSGSMGEFKKYIARSFFFWMVRFLRTKYDNVEIVFISHHTEAREVSEEQFFTQGES
                     GGTVVSSAYQLALDVIRERFRPADWNVYPFHFSDGDNYYSDNEEAVRLADELIQTCNL
                     FGYGEIGEEGAAGYRRSSGALLSIYNDRLKNRERFVGVRIDDKEDVYTALKQFFGSRG
                     VEV"
     gene            2032..5139
                     /locus_tag="VF240_19170"
     CDS             2032..5139
                     /locus_tag="VF240_19170"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7177387.1"
                     /translation="MRIGRLTEAFTKTLAVALLVAGLVAPADTLAQTANTGVITGTVQ
                     NEKGEVVPGATVRAINVGTNATRETQTSDEGVYEISQLVPGTYRLEVEATGFAKQVQS
                     DVVVNVLQRTTVNPALRVGGIGETVNVSADSASLIETSKTDVSGVIDQRRLENLPVNG
                     RSFASLAILIPGATLQPSFDPTKSRVGTFSVGGSTGRNVNVTIDGGDNKDNAVGGILQ
                     NFSMEGIQEFALSTQRFSAANGRSGGALLSVISKSGTNSMHGSLFGFFRDDKLNANAP
                     ALLAEARGEDTANAQKPPFSRQQFGGSIGGPIKRDKAFFFGSIERTRERGNSFVPAET
                     QAEISFLESFGYQAVPLLPQPFNDTQYTIKGDFTLNSRHTLVTRFAGQHNDALNDQAG
                     FLIVHTDLSGGNESLNTLYNFLASETWTVNQSTVNQFTYQYQTFDNRINATTDLNQLF
                     FPDGLLIGRNGNVPQQTIQKKHQFRDDLTWNRGNHGLKFGGDFTYVPTLGGLFAFNSA
                     PEYDFNFTAGEIANNPGQFPQGFRTTQILPGPITCSTFVGEPTCAAADLDGIGVVGAI
                     FLSGGDPSFNLREGAKQFAFYAQDDWKVRPNLTLNFGVRYDVDYGFVDNAHAAENRAF
                     RALQIIGSPFARKVVEDDTNNFSPRVGFAWDVRGDARSVLRGGYGIYYDQSFLNVPLF
                     AVQQANPEIYATFLNDGANLSIDSPPPDIPRPLNNPLSGTRGRMLDPDFESPYTQQWN
                     LGFAQELGSNMSVEFDYVHILGLHEFTGLDINPRIGPLINSQRSSGNPGRILTAQFAA
                     HAAELTAAFGTPTPMGRITVAQSDSRSRYDAFTVAFRKRYSNKYQLNAHYTLAKSQAW
                     FGAAADFGLQPQNLFDKWDPVNFGPTGEDERHRFVLSGIFDLPWGFQLAPIFQLASAR
                     PYSIFPACVCDINRDGVNNDRESVDPGVNDQVKLPPNTERGDSFSQLNVRVSKFFNFG
                     ERAKLGLFFEAFNVFNTGNFGNQYQNVTGSPDFGQPVNFFGATGFSEPIGIPFQAQFG
                     ARFSF"
     gene            5292..6317
                     /locus_tag="VF240_19175"
     CDS             5292..6317
                     /locus_tag="VF240_19175"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177388.1"
                     /translation="MPRRTFNRFPLAAFALILLAAAAAPTTNTNTIAHAQGANSLEGR
                     VMLPNGAQPTNSVKVTLVFNGRRMHETFTDLSGRFAFSGLGSGTYHLTAEGDGTTFET
                     TTVSADVGIFGSAQTYTQNIQLRLKAGATSTTPPGTISAAEVDPDVPARAREKYSQGL
                     RQAAENRPEQAAKLFEEAVREHAKFYAAALALGEQYAKLQRHDDALAAYRRATEIKPD
                     RAEAYVGVGVSLAGLKKFEEAVKLLRGVVELDKDLPGAYLPLGYAEMMTGDHAAAERH
                     LLRALELGRAALAHVYLAHVYEQTLQPAKAVEHLETYLKENPQTLNAAAVRGAIEKLR
                     KKLKSAK"
     gene            6472..7572
                     /locus_tag="VF240_19180"
     CDS             6472..7572
                     /locus_tag="VF240_19180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177389.1"
                     /translation="MKRAACFLSGTVVVAATFVVPGVAAGATRAKYVTPAVATQANRN
                     SIGGMVMGPDRRPLADVYVELASEYNSQIGPRQRTNSSGRYHFSNLPEGRYKVRVLSA
                     ASDFEEQTQEVVIQNAVRQLPGGGTFVSGFESAQLDITMRLRRPRSGEAPKNAPGTIF
                     AQVAPEGAKRAYEQAVADLDVKKDDAAGLAGLKKAVEMFPTYYDALLRLGTEHLRRQT
                     YTEALAALNKAVEVNPRGYAGLYGLGVAQHYLRQRAAAAETFRRAVNLNPGSVNAQIW
                     LGIALRLNGQFAPAEAALKRARELGGGGPVPEVHWQLSLLFHQQRRYNDAANELELFL
                     KASPDARDAAQIGNLIRQFREQARATKAKFTP"
BASE COUNT         1471 a         2716 c         2291 g         1233 t
ORIGIN      
        1 cttccggcag gagatactca tccgcatcag ctcctacgcc cgcaagggcc ggcagttcga
       61 gtacaactcg cacgagcgtt tgaaagaagc catcgagaag aaaatcttct ccgacctcaa
      121 ggacgtggtc aagatcacca cttccgtcaa gacgcccgac cccgaccagc tccgccgcat
      181 caacgaggtc gttgaccggc tcgtcaagga gcacggctac tgccccgtct gcgccaacga
      241 gctgctgacc tacgtcggca cgctgctctc acgctaatcg gcgcgcgcgg cgcgtccgtc
      301 gccagtgtaa ctagagacgc ccgcgctttt cgctccagaa ggcgcgggcg ttgtgattgg
      361 cagaagaaaa gttcaaatga cagaagactt catctcaggc accatccagc gcaacgactg
      421 gtcgttgcag cgtaagggcg tgatcgacca ggagcggcac cgggagcggg tcaaggaggc
      481 cgtgcgtcag aacctcggct ccatcgtctc taacgaagcc atcatcctct cggacggtcg
      541 gcgcacggtc aaggtgccga tccgctcgct cgacgagtac aagttccgct ttgaccatcg
      601 caagcgcaaa cacgtcgggc agggcgacgg caagacacgc gtcggcgacg tgatcgcgcg
      661 cgaggggcag cccggtcagg ggccggggca ggggtcgggg ccgggcggac agggcgctgg
      721 ggccgagtac tacgaggccg aggtgaacat cgacgagctg gccgcgatga tctttgaaga
      781 cctccgcctc ccgttcctcg aagagaaggc gaagaaggcc gtgcaggcgc gcaccacgcg
      841 cttcaacgag attcgccgca ccggcgtgat gtcgaacctc gacaagcggc gcatgattct
      901 ggagaacatc aagcgcaacg cgcgcgagga cggccgcccg agtttcaagg gcgtccgcaa
      961 ggaagacctc cgcttcaagt catgggaaga ggagaaccgc tacgagtcga acgccgtggt
     1021 gctcgcgttg atggatgtct cggggagcat gggcgagttc aagaagtata tcgcgcgctc
     1081 cttcttcttc tggatggtgc gtttcttgcg gacgaagtac gacaacgtcg agatcgtttt
     1141 catctcgcac cacacggagg cgcgcgaggt ctcggaggag cagttcttca cgcagggcga
     1201 gtcaggcggc acggtcgtct cctcggccta ccagctcgcg ctcgacgtga tccgcgaacg
     1261 cttccgcccc gccgactgga acgtctaccc cttccacttc tccgatggcg acaactatta
     1321 ctcggacaac gaggaggccg tccgcctcgc cgacgagttg atacagacct gcaatctctt
     1381 cggctacggc gagatcggcg aggagggagc cgccggctac cgccgctcct cgggcgccct
     1441 gctttcgatc tacaacgacc ggctcaagaa ccgcgagcga ttcgtcggcg tgcgaatcga
     1501 cgacaaggaa gacgtctaca cggccctcaa gcagttcttc ggcagccgcg gcgtggaggt
     1561 ctgaagtcgt gacggggcgg cgagcctccg cgtgggtgag tgcgccgggt ctccgaaaaa
     1621 atggagaaaa atgcgaaagt tcgcttccca ttgccgcttc ggcgcctcac gtccgatgag
     1681 ataaagtgta gaagcttaga gaattaaccc tccacgccgg cccctgccct ccggtggaat
     1741 attgtttgcg tcgttgaaac ctcagctagc ctatcgaacc cgggtctggg aagttttaat
     1801 attttgccgc gtctgtgacg cttgatcgcg gccctcgccg atgccgccgg tcgagccgcg
     1861 cccccgacgc acggccggca gttcatcaat cctgtttcac agggtctcat catcagtggt
     1921 tttcagacca gaccatcccc gcacggtgac atccggcagt ttagttagcg cccggtctga
     1981 agccacatcg ttcaccaatc tgaccaaata actcacgata ggaggccttc aatgaggata
     2041 ggtagactca cagaagcgtt tacgaagacg ctcgccgtcg ccttgctggt ggcggggctt
     2101 gtcgcgcccg ccgacacgct ggcgcagacg gccaacacgg gcgtcatcac cggtacggtt
     2161 cagaatgaga agggcgaagt ggtgcccggc gcgaccgtcc gggccatcaa cgtcggcacc
     2221 aacgccacgc gcgagacgca gacgagcgac gagggcgtct acgaaatttc gcaactcgtg
     2281 cccggcacct accgcctcga agtcgaggcc acgggcttcg ccaagcaggt gcagagtgat
     2341 gtcgtggtca acgtgctcca gcgcaccacg gtcaacccgg cgctccgggt cggcggcatc
     2401 ggcgaaactg taaacgtctc ggccgatagc gcctcgctta tcgagacctc gaagacggat
     2461 gtctccggcg tcatcgacca gcggcggttg gagaacctcc ccgtcaacgg ccgctcgttc
     2521 gcctcgctcg ccatcctcat ccccggcgcg accctccagc cctctttcga cccgaccaag
     2581 tcgcgcgtcg gcaccttctc cgtgggcggc tcgacgggcc gcaacgtcaa cgtcaccatc
     2641 gacggcggcg acaacaaaga caacgccgtc ggcggcatcc tccagaactt ctcgatggag
     2701 ggcattcagg agttcgcgct ctccacccag cgtttctcgg cggccaacgg ccgctcgggc
     2761 ggcgcgctcc tgtcggtcat ctcgaagtcg ggcacgaaca gcatgcacgg ctcgctcttc
     2821 ggcttcttcc gcgacgacaa gctgaacgcc aacgctccgg cgctgctggc cgaagccagg
     2881 ggcgaggaca cggctaacgc gcaaaagccc cccttcagcc gccagcagtt cggcggttcc
     2941 atcggcggcc cgatcaagcg cgacaaggcg ttcttcttcg gcagcatcga gcgcacgcgc
     3001 gagcgtggca actccttcgt tccggccgag acccaggcgg agatctcctt ccttgaatcg
     3061 ttcggctatc aggccgtccc gctgctgccg cagcccttca acgacacgca gtacacgatc
     3121 aagggcgact tcaccctcaa ctcgcgccac acgctcgtga cgcgcttcgc cggtcagcac
     3181 aacgacgccc tgaacgacca ggccggcttc ctcatcgtcc acaccgacct ctcgggcggc
     3241 aacgagagcc tgaacacgct ctacaacttc ctcgcctccg agacctggac ggtgaatcag
     3301 agcaccgtca accagttcac ctaccagtac cagaccttcg acaaccgcat caacgcgacg
     3361 accgacctca accagctctt cttccccgat ggcctcctca tcggccgcaa cggcaacgtc
     3421 ccgcagcaga ccatccagaa gaagcaccag ttccgcgacg acctgacctg gaaccgcggc
     3481 aaccacggcc tcaagttcgg cggcgacttc acctacgtcc cgaccctcgg cggcctgttc
     3541 gccttcaact cggcgccgga gtacgacttc aacttcaccg ccggcgagat cgccaacaac
     3601 cccggccagt tcccgcaggg gttccgcacg acccagattc tgccggggcc gatcacctgc
     3661 tcgacgttcg tcggcgagcc gacctgcgcg gcggccgacc ttgacggcat cggcgtcgtg
     3721 ggcgccatct ttctctcggg cggcgacccc agcttcaacc tgcgcgaggg cgccaagcag
     3781 ttcgccttct acgcgcagga cgactggaag gtcaggccca acctgacgct caacttcggc
     3841 gtgcgctacg acgtggacta cggcttcgtc gataacgccc acgcggccga gaaccgcgcc
     3901 ttccgcgcgc tccagatcat cggcagcccg ttcgcgcgca aggtggtcga agacgacacg
     3961 aacaacttca gcccgcgcgt cggcttcgcc tgggacgtgc gcggcgacgc ccgctcggtg
     4021 ctgcgcggcg gctacggcat ctactacgat cagtcgttcc tcaacgtgcc gctcttcgcg
     4081 gtgcagcagg cgaacccgga aatttacgcc accttcctca acgacggcgc gaacctcagc
     4141 atcgattcgc cgccgcccga catcccgcgc ccgctaaata accctctgtc gggcacgcgc
     4201 ggcaggatgc tcgaccccga cttcgagtcg ccctacacgc agcagtggaa cctggggttc
     4261 gctcaggaac tcggcagcaa catgtccgtg gagttcgact acgtccacat cctcggcctg
     4321 cacgagttca cgggcctcga catcaacccg cgcatcgggc cgctcatcaa ctcccagcgc
     4381 agcagcggga acccgggccg catcctgacg gctcagttcg cggcgcacgc cgccgagctg
     4441 accgccgcct tcgggacgcc cacgcccatg gggcgcatca ccgtggcgca gtccgacagc
     4501 cgctcccgtt acgacgcctt caccgtggcc ttcaggaaac gctactcgaa caagtatcaa
     4561 ctgaacgcgc actacacgct ggcgaagtcg caggcgtggt tcggcgcggc cgccgacttc
     4621 ggcctccagc cgcagaacct gttcgacaag tgggaccccg tcaacttcgg ccccacgggc
     4681 gaggacgagc ggcaccgctt cgtgctctcc ggtatcttcg atctgccgtg gggcttccag
     4741 ctagcgccga tcttccagtt ggcgtcggcc cggccctaca gcatcttccc ggcctgcgtc
     4801 tgcgacatca atcgcgacgg cgtgaacaac gaccgcgagt cggttgaccc cggcgtgaac
     4861 gatcaggtca agctgccgcc gaacacggag cgcggcgaca gcttctcgca gttgaacgtc
     4921 cgcgtctcga agttcttcaa cttcggcgag cgggccaagc tcggcctgtt ctttgaagcc
     4981 ttcaacgtct tcaacacggg caatttcggc aaccagtacc agaacgtgac cggctcgccg
     5041 gacttcggcc agccggtcaa cttcttcggc gccacgggct tctccgaacc catcgggatt
     5101 ccgttccagg cgcagttcgg ggcgcggttc agcttctaac cagaggccgc gaacccttca
     5161 gaccgggccg gggcgcccgc gccaacttcc gggttagcgc gggcgcccca cccttctttt
     5221 tgctttcgcg aatcgggcat tacgcacgcc gccgaaccgt ccgcgcgctc cgcacgtggt
     5281 atagatcaaa catgcccaga cgaacgttca accgattccc actcgcggcc ttcgcgctta
     5341 tcctgttggc ggccgccgcc gcccccacca ccaacaccaa caccatcgca cacgcgcagg
     5401 gagccaacag ccttgagggc agggtcatgc tgcctaacgg ggcgcagccc acaaactccg
     5461 tcaaggtcac gctcgtcttc aacggccgtc ggatgcacga gaccttcacc gacctcagcg
     5521 ggcgattcgc cttctcgggc ctcggctccg gcacttatca cctcaccgcc gagggtgacg
     5581 gcacgacctt cgagacgacg accgtcagcg ccgacgtggg catcttcggc tccgcgcaga
     5641 cctacacgca gaacatccag ctccgcctga aggccggcgc tacgtccacc acgccgcccg
     5701 gcacgatctc cgccgccgag gttgacccgg acgtgcccgc gcgcgcgcgg gagaaataca
     5761 gtcagggact caggcaggcc gccgagaaca ggcccgagca ggcagcgaaa cttttcgagg
     5821 aggccgtccg ggaacacgcg aagttctacg ccgccgccct cgcgctcggc gagcagtacg
     5881 ccaaactcca gcgtcacgac gacgcgctcg cggcctaccg cagagcgacc gagatcaagc
     5941 ccgaccgcgc ggaggcttac gtcggcgtcg gggtcagcct cgccggcctg aagaagttcg
     6001 aggaggccgt caagctgttg cgcggggtcg tcgagttgga caaagacctg ccgggcgctt
     6061 acctgccgct cggctatgcc gagatgatga ccggcgacca cgccgccgcc gagcgccacc
     6121 tgttgcgcgc gctcgaactc ggccgcgccg cgctcgcgca cgtctatctc gcccacgtct
     6181 acgagcagac gctccagccg gcgaaggccg tcgaacacct tgagacttat ctcaaagaga
     6241 atccccaaac gcttaacgcc gcggcggtgc gcggcgcgat tgagaagctt cgtaaaaagc
     6301 tcaaaagcgc gaagtaaggg gcgccggcga aggccgcccc cttcgagatg cgccgggtga
     6361 tatttgtgcc atgtgcttcg cgcctcgtga tatcatcaga catccccgga aacactagga
     6421 atgatttgta acccgatctg cgctatcttc aaatttaagg agttgagatt tatgaaacgg
     6481 gctgcctgtt ttctatccgg cacggtcgtc gtggccgcca cctttgtcgt ccccggcgtc
     6541 gcggccggcg ccacccgcgc gaagtacgtc acgcccgccg tcgccaccca agccaaccgc
     6601 aactccatcg gcgggatggt gatggggccg gaccgccgcc cgttggccga cgtgtacgtc
     6661 gagctggcga gcgagtacaa ctcccagata ggcccgcgac agaggacgaa ctcctccggc
     6721 cgctaccact tcagcaatct ccccgagggg cgctacaaag ttcgcgtgct ctccgccgct
     6781 tcagacttcg aagagcagac gcaggaagtg gtcatccaga acgccgtccg ccagctcccc
     6841 ggcggcggca ccttcgtctc cggcttcgag tccgcccagt tggacatcac catgcgactc
     6901 aggaggccgc gcagcggcga ggccccgaag aacgcccccg gcacgatctt cgcccaggtg
     6961 gcgcctgaag gggcgaagcg cgcctacgaa caggccgtcg ccgacctcga cgtgaagaag
     7021 gacgacgcgg ctggcctcgc cgggctgaag aaggccgtcg agatgttccc gacttactac
     7081 gacgcgctcc tgcgtctggg gacggagcat ctgaggcggc agacgtacac ggaagcgctc
     7141 gccgcgctca acaaggccgt cgaggtcaat ccgcgcggct acgccggcct gtacggcctc
     7201 ggcgtcgccc agcactacct gcggcagcga gccgccgccg ccgagacctt ccgccgcgcc
     7261 gtcaacctca accccggctc ggtcaacgcg caaatctggc tgggcatcgc gctccgactc
     7321 aacggacagt tcgcccccgc cgaggcggcg ctcaagcgtg cgcgcgaact cggcggcggc
     7381 ggccccgtgc ccgaagtcca ctggcagctc tccctcctct tccaccagca gcggcgctac
     7441 aacgacgcgg ccaacgaact cgaactgttc ctcaaggcgt cgcccgacgc gcgcgacgcc
     7501 gcgcagatcg gaaatctcat ccgccagttc cgcgaacagg cgcgagccac caaagccaaa
     7561 ttcacgccat gaatctttga gcctccggcg caaggcgacg ctgtgacggg gcatgacgac
     7621 gaagcgggtt cgagaccagt ccgcaagcca cttggctgac atcatggagg cgggccgcgg
     7681 cgcgccggct caagacccga tgcctgagcc g
//
LOCUS       DASVES010000462         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1566436, whole genome shotgun sequence.
ACCESSION   DASVES010000462 DASVES010000000
VERSION     DASVES010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566436"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1084
                     /locus_tag="VF240_19185"
     CDS             179..1084
                     /locus_tag="VF240_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177390.1"
                     /translation="MNAANKHRSLHPFAPGRSFAASALLFFACIAVSQAQQRPLITED
                     VDVLPPGVIRIEAGIDFMQDAQFPLSGLTGDLTRIGVIGINIGLSPNVEFQIEGVAQN
                     FLSINTANPNPAVLRGFVPGANSTNGTGDFTLATKIKIRNEGRRSPSLGFRFGVELPN
                     SNETRGIGVNQTNAFGVLLVGKKFGRRQRLNTFGNIGIGILTAPLAQFTQNDVLLYGA
                     AGIFRLNNHVNIAAEVNGRASTRAGRAPLGTEPLSEARVGVQVKAAGLRFDFAGIKGL
                     TDFSPRSGVTFGVTYDTPTVFAPAK"
     gene            1173..1874
                     /locus_tag="VF240_19190"
     CDS             1173..1874
                     /locus_tag="VF240_19190"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7177391.1"
                     /translation="MSEWLAATAKWLHDFIVGVPIYYAGPAMVLIGALDSSLLSLPEI
                     NDYLVVARCFSDPKAVFYFPLFAAAGSVIGCMVLYSIMRRGGQAVMRRRFRPESIARV
                     ERAYARFGFLALAVPALLPPPMPFKVFVATAGALEYPRWRFMLTILIARSLRYYVEGA
                     LAVFYGEQVIDFIRHYGLKLLIVVAGLVLIGLAVYFTTKRLRARGTKPDEVAQAERVE
                     GRGQIEDVGEPAEKL"
     gene            1871..2512
                     /locus_tag="VF240_19195"
     CDS             1871..2512
                     /locus_tag="VF240_19195"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7177392.1"
                     /translation="MKWLWRKITNGLTSVTDYLVTLGPAGLFAVALLDSALVPLPGGP
                     DAVMIALTMARPSWMPLNVAAAALGSTAGCVILYYISKRAGRRALEKFSERKQARVKE
                     LVDRYDVLSVLVASLLPPPFPFKLFVITAGVFRLNILRFALAVAAGRTVRFLLEGYLA
                     VRYGEQAKQVLADNYPVVGLGLAALIVLVFLVRALLKKKREGAGGGEGEVSEG"
BASE COUNT          434 a          844 c          788 g          496 t
ORIGIN      
        1 agttcaacgc gaagaaggaa atgcagggca tgaaggtggt cttcaactgg agaatctttc
       61 tcgccgggcg acgcctgtat cagttggccg tcgtgacgca ggacacggcc gcgccgtcgc
      121 ccgagatcgg aaagttcttc acgtcgttca atatcacaaa gtaggtggcc ggcagttgat
      181 gaacgcagca aataaacatc gcagcttgca cccattcgcg ccgggccgaa gcttcgccgc
      241 gtcggcgctt ctgtttttcg cgtgcatcgc cgtcagtcag gcgcagcagc ggcccctcat
      301 caccgaggac gtggacgttt tgccgccggg cgtcatccgc atcgaggccg gcatcgactt
      361 catgcaggac gcgcagtttc cgctttcggg gctgacgggc gacctgacgc gcatcggcgt
      421 catcggcatc aacatcggac tctcccccaa cgtcgagttc cagatcgagg gcgtggcgca
      481 aaacttcctg agcatcaaca cggccaatcc caatccggcg gtgctgcgcg gcttcgtgcc
      541 cggcgccaat tcgacgaacg gcaccggcga ctttacgctc gccaccaaga ttaagattcg
      601 caacgaaggg cgacgctcgc cctcgctcgg ctttcgcttc ggcgtggagc tgccgaactc
      661 gaacgagacg cgcggcatcg gcgtcaatca gacgaacgcc ttcggcgtcc tcctcgtcgg
      721 caagaagttc ggccggcggc agcgtctcaa caccttcggc aacatcggca tcggcatcct
      781 caccgccccg ctcgcccagt tcacacagaa cgacgtgctg ctctacggag cggccggcat
      841 cttccgcctc aacaaccacg tcaacatcgc cgccgaggtc aacggccgcg ccagcacgcg
      901 cgcgggccgc gccccgctcg gcaccgagcc gctttccgag gcccgcgtcg gagtccaggt
      961 gaaggccgcg ggcctgcgct tcgacttcgc cggcatcaag ggcctgaccg acttcagccc
     1021 gcgctccggc gtcaccttcg gcgtcaccta cgacaccccc acggtcttcg cgccagcaaa
     1081 gtgaatcggt gataagtgat gagtgataag tgataagaaa gaaatctgtt tttcttatca
     1141 cttatcactc atcacttatc actgctctta ctatgtccga atggttagct gcgacggcga
     1201 agtggctgca cgacttcatc gtgggcgtgc ccatctatta cgcggggccg gcgatggtct
     1261 tgatcggcgc gctcgactcc tctctgttga gtctgccgga gatcaatgac tacctcgtcg
     1321 tcgcgcgttg cttcagcgac ccgaaagccg tcttttactt cccactgttc gcggcggccg
     1381 gctccgtcat cggctgcatg gtgctctact ccatcatgcg tcggggcgga caggccgtga
     1441 tgcgccgtcg tttccgcccc gagagcatcg cgcgcgtgga gcgggcttac gcgcgcttcg
     1501 gattcctcgc gctggccgtg ccggcgctgc tgccgccgcc gatgcctttc aaggtgttcg
     1561 tcgccaccgc cggcgcgctc gaatacccgc gctggcgctt catgctcacg atcttgatcg
     1621 cgcgcagcct tcgctactac gtcgaagggg cgctggccgt cttctacggc gaacaggtca
     1681 tcgactttat caggcactac gggctgaagc tgctgatcgt ggtcgcgggc ctcgtcctca
     1741 tcggcctcgc tgtgtatttc acgacgaagc gtctgcgcgc gcggggaacg aagccggacg
     1801 aagttgcaca agcagaacgt gtcgaaggac gcggccagat tgaagacgtt ggagagccgg
     1861 cggaaaaatt gtgaagtggc tctggcgaaa gattacgaac gggctaacgt ccgtcaccga
     1921 ctacctcgtc acgcttggcc cggcgggtct cttcgccgtc gctctgctcg actcggcgct
     1981 cgtgccgctt ccgggcggcc ccgacgccgt gatgatcgcg ctgacgatgg cgcgaccctc
     2041 atggatgccg ctcaacgtgg ccgccgccgc cctcggctcg acggccggct gcgtgatcct
     2101 ttactacatt tccaaacgcg cgggccggcg tgctctcgaa aaattctccg agcggaagca
     2161 ggcgcgcgtc aaagagctgg ttgaccgcta cgatgtgctc tccgtcctcg tcgcgtcgct
     2221 gctgccgccg ccgttcccct tcaaactctt cgtcatcacg gccggcgtct tccgcctcaa
     2281 catcctgcgc ttcgcgttgg cggtcgccgc ggggcgcacc gtccgcttcc tgctcgaagg
     2341 ctatctcgcc gtgcgctacg gcgaacaggc gaagcaggtg ctcgcggaca actatcccgt
     2401 agtcggcctc gggctggcgg cgctcatcgt gttggttttt ttggtccgcg ccctgttgaa
     2461 gaaaaagcgg gagggcgccg gcggcgggga gggggaagtt agtgagggct gacattgatt
     2521 caccgcagag gcgcggagga cgcggagggg gcgcagaggg tt
//
LOCUS       DASVES010000463         7896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_862454, whole genome shotgun sequence.
ACCESSION   DASVES010000463 DASVES010000000
VERSION     DASVES010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862454"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..1266
                     /locus_tag="VF240_19200"
     CDS             592..1266
                     /locus_tag="VF240_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177393.1"
                     /translation="MRRAAKHLRRARFFSSNYVRWAIFLVAIWLFVVPFFLIIVGSEF
                     RFYDSFIVSLGNWFTFSFGSLILWILILCFTGGMLSKVHQELPTIDGGEKVGEAETAE
                     CAMCGGAIAYEKGDLATVCGYCGVETYRVRVAWQARKSATQSRQAATFSLVEAMQVSK
                     EKVDDLISTPAILVFIFVVCPFFMIFVPYLVYTFVTENLLISIPLIVCLAVAAYLLKR
                     FYFRKA"
     gene            1287..2342
                     /locus_tag="VF240_19205"
     CDS             1287..2342
                     /locus_tag="VF240_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177394.1"
                     /translation="MAYLYQSYVAISLLQGISLPFLIIIFRALSVRHRQKRASLKKEL
                     EVIRGRKTKTTEISRLVQDDLPKLQPLKCRACGAGVLLKETETFCPHCETRSNLPEDY
                     AAAVSLRREVKKLLKSAVRHWRAANILTRPLLTGLFVSLGVAGPLLALFLPVIIQTAG
                     GTLPNTVADSLMASLGTPLDGIVYLFWISGIIIWTLMFLCLAVTGSDLRKKLPVVPVL
                     EEKIRGGETADCHTCGGAIEYEKDDFASICSYCNVGNFRVRFTRLKRAEIEDRKTQTS
                     FALFGAMEIIENYVTLIYAGALIFFGFPALLFILGVAIYAAANGSLVLSMSAFLVLAV
                     LIASGRFIISEERQIHQ"
     gene            2369..2707
                     /locus_tag="VF240_19210"
     CDS             2369..2707
                     /locus_tag="VF240_19210"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEX7177395.1"
                     /translation="MKALRLILAVMLLAMLGAALPLSQLVPFPAAHGLDVPLHFRQLF
                     ETHASAFFVLDVIVSSLVLWLFVFSEGRRRGMKNLWLYVVCHRAVGVSLALPLFLFFR
                     ERRLERTGGA"
     gene            2704..3552
                     /locus_tag="VF240_19215"
     CDS             2704..3552
                     /locus_tag="VF240_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177396.1"
                     /translation="MSKYYDTHPHEHPVLADIRQKYFPYTWSMFPSEFRIYYQACPVR
                     LPGGGNTNVGILKYVSGWNEAGGGQKYTLGRHVRKYYGEGINPELDDWLSTTRPTAVF
                     AGHGLPEEISLVCSLAMATGYKTAGEIGPWATKYIGLDCNGFVSAYLISLGTFSRVLH
                     DHPSYPSVTKLAQSVSEISYDSVILWSKKEGGKYKVVQNPSKSAHIAVVDGWDEYGSS
                     LLVTERGGESRFPGTVGIHYGIYDIIDAPPAGCSAAGAGWVIRAREHKSYDTDTVVIT
                     RTMEAN"
     gene            3662..4159
                     /locus_tag="VF240_19220"
     CDS             3662..4159
                     /locus_tag="VF240_19220"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7177397.1"
                     /translation="MKDALEDFRRTVETAAESLLGMTDEQCERRSAPGKWSAKEVVGH
                     LIDSAANNHQRFVRAQFQDDLAFPGYAQEEWNAAQHYREEPWRQLVELWRLYNLHLLH
                     VVSHITEEDLRRPRRPHTLHRIAWKLVEESEPATLEYLIRDYIEHLKHHLGQIARADT
                     ERENT"
     gene            4160..4870
                     /locus_tag="VF240_19225"
     CDS             4160..4870
                     /locus_tag="VF240_19225"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7177398.1"
                     /translation="MKSFVHRAVFALAACLVFGTAAAAQEEKKEEYDMGLTQLALLSR
                     APDWKAEAAAPHVEKAQRAYVERLIEDGTAALAGSVAGDGDLREILVFKTDSTELASE
                     WVRNSPAVKAGVLKAEVLKWYVARNYITPPRRPLARTDYVFGILVRGPKWTPEETEET
                     KKLQAGHMANIGRLAKEGKLVLAGPFEDGGERRGVFIFKAPTLEEAQRLTDTDPAVAA
                     GRLKIQLHKWSVPEGMLR"
     gene            4939..5451
                     /locus_tag="VF240_19230"
     CDS             4939..5451
                     /locus_tag="VF240_19230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7177399.1"
                     /translation="MIVVRAERAEDAEGVRRVNELAFEGAAEADLVDALRVNAHPYVS
                     LVAVEEGGEVAGHIFYSPVTVEPEAGELLLMGLGPMAVLPRVQKSGVGSRLVKEGLDE
                     CRRLGCAAVVVLGHPTYYPRFGFAPASRLGLRCSYEVPDEVFMAMELEPGALDGRRGL
                     VKYHPAFDAV"
     gene            5568..7271
                     /gene="recN"
                     /locus_tag="VF240_19235"
     CDS             5568..7271
                     /gene="recN"
                     /locus_tag="VF240_19235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX7177400.1"
                     /translation="MLAVLNITNFALIDDLSVEFERGLNLLTGETGSGKSILVDALGV
                     LIGGRFSSEMVKAGAAQASVEGLFSVGENPRVEELLGAAGLDARPDGGAAELVIRREL
                     AAGGRGRIFINNRLATLALLRELRPFLVDIHGQGDQQTLFNPDTHLELLDAFGGHDPA
                     RREVSKLYALWAALNRELEDLRRDDAEKLQLIDALRFQTEELEAARLSSGEEEQLASE
                     RRLLGNLEKISALCADAYDKTYERDDSTVARLSQLTHQVEELAGYEQSYRDYLEGVRS
                     ARVLLEDLGLSLRSFADHLKFSPERLAEIESRLAEVARLKRKYGGTVEQAVEHLARSR
                     ERLDHLERSDERAAEVTKELTAARATYLAAARKLHDARVKTARAFGRAVERALTEVAM
                     ESAKFAAEFETPGAGADERALTKSFTAAGIDRVELHFSANPGEPPRPLARVASGGEAS
                     RLMLVLKTMINPSRFPRTIVFDEIDAGIGGRVSEAVGLKLKTLAGANQVLCVTHQAQV
                     ARFADTHLLVTKTAERGATSVGVRKLDGRGRVEEIARMLTGAQITETARRHAREMLKT
                     A"
     gene            complement(7365..>7896)
                     /locus_tag="VF240_19240"
     CDS             complement(7365..>7896)
                     /locus_tag="VF240_19240"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX7177401.1"
                     /translation="DELLDVILTDGTKRIFIATHNGLAIRFEEDDVRSMGRAARGVRG
                     IDLRKDDFVVSVCAVSSDDSERMLSVSECGFGKQTPISTYRLQSRGGKGVINMKTTDK
                     TGKVVAVFPVEDDSELMIITQHGKLIRIEASEIRKTGRGAQGVRLIKTDGGDKVTSSS
                     LLAPMNEDELVAVVAE"
BASE COUNT         1426 a         2416 c         2531 g         1523 t
ORIGIN      
        1 ccggagcaga gaaaagaatg gcggttgatt ggcggcggga tcggcattca ttgggaagca
       61 gtggatgaag acatctcagt cgagagcctg ctcaggttga agtagactgc acccgaccat
      121 ctaacaactc attcaacccg ccgcctcgat agcttctctt tacatcgtta ttgggtttct
      181 tcatcttgaa tatttcatgc gcgacgtgag ttgattccgg cgtcagttcg cctcctctat
      241 ccgtggacga aataatcact tgatttcaag atgctgaacg tagccctaca cgtcgcggtg
      301 ctcacactcc tgctgcaagg atttcttttt ccgctcctcg tgctcgtgtc tcttcattgg
      361 acgtggcgtg cccttcatga caagggcaaa tccagagagg aaaaacagac gagatcggtg
      421 ggggaaaagg aaacgcgtgc gcccgctctg cggcctttga agtgtcccgg ctgcggcgcg
      481 ggggtgccgt tgcgggaagc tgagacggcg tgcgggggtt gcggaactct ggtcgccgtt
      541 cctcagcatt acgccgacat cggcaggctg aggaacgagg cggtcgcacg cttgagacgc
      601 gccgcgaagc atctgagacg ggcgcgcttc ttctcgtcga attacgtccg ttgggcgatc
      661 tttttagtcg ccatctggtt gttcgtcgtg cctttctttt taatcatcgt gggaagcgag
      721 ttcaggttct acgacagttt tatcgtgtcg ctcggcaact ggtttacttt ctcattcggc
      781 agcctgatcc tgtggattct catactctgt ttcaccgggg ggatgctcag taaagtccat
      841 caggagctgc cgacgattga cgggggcgaa aaagtcggcg aggcggagac ggcggagtgc
      901 gcgatgtgcg gcggcgcaat cgcctatgag aagggcgatc tggcgaccgt gtgcggctat
      961 tgcggggtgg agacgtatcg cgtcagggtg gcgtggcagg cgcgcaaatc ggcaacgcaa
     1021 tcccggcagg cggcgacctt ctcgctcgtc gaagcgatgc aggtctctaa ggagaaggtc
     1081 gatgacctca tctccacgcc ggcaatctta gttttcattt tcgtcgtctg cccgtttttc
     1141 atgatcttcg ttccttacct cgtttacacc ttcgttacgg agaatctact gatctcgatc
     1201 ccgctcatcg tgtgtctggc cgtggccgcg tatctgctga aacgctttta ttttcgcaag
     1261 gcgtaatctc cgagcttccg ccacgaatgg cttacctgta ccaaagctac gtcgcgattt
     1321 ctctgttgca aggaatctct ttgccgttct tgataatcat ctttcgcgcc ttgtcggtga
     1381 gacatagaca aaaacgcgct agtctcaaaa aagaactgga agttatccgc ggacgcaaaa
     1441 ctaaaaccac tgagatttcc agactcgttc aggacgactt gccgaagctc caaccgttga
     1501 agtgccgtgc ctgcggcgcg ggtgtgttac tcaaagagac cgaaacattt tgtccgcatt
     1561 gtgagacgcg gagcaatctg ccggaggatt acgcggcggc ggtttcgctc agacgggagg
     1621 ttaaaaaatt actcaaaagc gcggtcagac attggcgagc ggcaaacatt ttaacgcgtc
     1681 cgttgctgac cgggcttttt gtttcgctgg gtgttgcggg gccgctgctg gcgctttttt
     1741 tgcccgtgat tatccagacc gccggcggca cgctcccaaa cactgtggct gatagcctca
     1801 tggcgtcgct cggcacgccg ctggatggaa ttgtctatct gttttggatt tccggcatta
     1861 tcatctggac gctgatgttt ctctgcctcg cggtgacggg ttcggatttg cgtaaaaagc
     1921 tgcccgtcgt gcctgtgttg gaagaaaaga ttcgcggcgg tgaaacggcc gactgtcaca
     1981 cctgcggcgg cgcgattgaa tacgagaaag atgatttcgc cagcatttgt agttactgca
     2041 acgtgggaaa ctttcgcgtg cggtttaccc gactcaagcg cgccgaaatc gaagaccgga
     2101 aaactcagac gagttttgcg ctgttcgggg caatggaaat catcgaaaat tatgtcacct
     2161 tgatctatgc cggggcgttg atattttttg gcttccccgc gttgcttttt attctgggtg
     2221 tggcgattta cgcagccgcg aatgggtcgc tcgtattatc aatgtcggca ttcctggtgc
     2281 ttgctgtttt gattgcttca ggtcgcttta taatttctga agaacgacag attcatcagt
     2341 gacggaaagc tggtagtgga agtcacacgt gaaagccttg agactcatcc ttgcggtaat
     2401 gcttctggcg atgctcgggg cggcgttgcc gctgtcgcag ctcgttccgt ttccggcggc
     2461 gcacgggctt gacgtgcctc tgcacttccg gcagctcttc gagacccacg cctccgcctt
     2521 cttcgtcctc gatgtcatcg tctcctcgct cgtgctgtgg ctgttcgtct tctcggaggg
     2581 gcggcggcgc ggcatgaaga atctttggct ctacgtcgtg tgtcaccggg cggtgggcgt
     2641 gtcgctggcc ctgccgctgt ttcttttctt ccgcgagcgc cggctcgaac gcacaggagg
     2701 ggcatgagca agtattacga cacgcacccg cacgagcatc ccgtcctcgc cgacatcagg
     2761 cagaagtatt tcccctacac gtggtcgatg ttcccgtccg agttccgcat ctactatcag
     2821 gcgtgccccg tccgcctgcc gggcgggggc aacacgaacg tcgggattct gaaatacgtg
     2881 tccgggtgga acgaggccgg cggggggcag aagtacaccc tcggcaggca cgtccgcaaa
     2941 tactacgggg agggcatcaa ccccgagctg gacgactggc tttcgacgac gcggccgacc
     3001 gccgtctttg ccggccacgg cctgccggaa gagatttcgc tcgtctgctc gctggccatg
     3061 gcgaccggct acaagacggc cggcgagatc gggccgtggg cgacgaagta catcggcctc
     3121 gactgcaacg gcttcgtcag cgcctacctc atctccctcg gcaccttctc ccgcgtgctg
     3181 cacgaccacc cctcctaccc gtccgtgacc aagctcgcgc agtcggtctc ggaaatctcc
     3241 tacgacagcg tcatcctgtg gtcgaagaag gagggcggta agtacaaggt ggtgcagaac
     3301 ccgagcaaga gcgcccacat cgccgtcgtt gacgggtggg acgaatacgg cagcagcctg
     3361 ctcgtcaccg agcgcggcgg cgagagccgc ttccccggca ccgtcggcat ccattacggc
     3421 atctacgaca tcatcgacgc cccgccggcc ggctgctcgg cggccggggc cggctgggtc
     3481 atccgcgcac gcgagcacaa gtcgtatgac acagacacgg tcgtcatcac gcgcacgatg
     3541 gaggccaact gaagccgcgc gtcgccgccg aagaggcaag tgccgcacgc tgactgccct
     3601 cgtgcatccc gcagttgaac tgagcaggcg agtcgggcgc gcgcgtcgcg ggtgacgaaa
     3661 gatgaaagac gcactggaag attttcggcg gacggtggag acggccgcgg agtcgctgct
     3721 cgggatgacg gatgagcagt gcgaacggcg gagcgcgccg ggcaagtggt cggcgaagga
     3781 ggtggtcggc catctcatcg actcggcggc gaacaaccac cagcgtttcg tccgcgcgca
     3841 gtttcaggac gacctcgcct ttcccggcta cgcgcaggaa gagtggaacg cggcgcagca
     3901 ctacagggag gaaccgtggc ggcaactcgt cgagttgtgg cggctctaca acctgcacct
     3961 gctccatgtc gtctcgcaca tcacggaaga agatttgcgg cggccgcggc gcccgcacac
     4021 gctgcaccgc atcgcgtgga agctcgtcga agagagcgag ccggcgacgc tcgaatacct
     4081 gatacgcgac tacatcgaac acctcaaaca tcatctcggc cagatcgccc gcgccgacac
     4141 cgaacgggag aacacgtaaa tgaagagttt cgttcacagg gctgtcttcg cgctcgcggc
     4201 ctgcctcgtc ttcgggacgg cggccgccgc gcaggaggag aagaaggaag agtacgacat
     4261 ggggctgacg cagttggcgc tgctcagccg cgcgcccgac tggaaggccg aggccgccgc
     4321 gccgcacgtc gaaaaggccc agcgggcata cgtcgagagg ttgatcgagg acggcacggc
     4381 ggcgctcgcc ggcagcgtcg cgggcgatgg cgacctgcgc gagattctgg ttttcaagac
     4441 ggactcgacg gagctggcga gcgagtgggt gcgaaactcg ccggccgtca aggcgggcgt
     4501 gctcaaggcc gaagtgctca agtggtacgt cgcgcgcaac tacatcacgc cgccgcgccg
     4561 tccgctcgcg cgcactgatt acgtcttcgg catcctcgtg cgcgggccga agtggacgcc
     4621 ggaagagacc gaggagacga agaagctaca ggcgggccac atggcgaaca tcggccgcct
     4681 cgccaaagag ggcaagctcg tgctcgccgg cccgttcgag gacggtggcg agcgccgcgg
     4741 cgtctttatc ttcaaggcgc cgacgctcga agaggcgcag agactcaccg acaccgaccc
     4801 ggccgtcgcc gccggacgcc tcaagattca actgcacaag tggtctgtgc ccgaggggat
     4861 gctgcgctga gtcgtcagct cttgtgggct acaagccgcg gcccacgccg agtgtgcggg
     4921 gtgaggaggg aggcatgaat gatagtcgtc agggctgaac gggcggaaga cgcagaaggt
     4981 gtgcggcgcg tgaacgagct ggcgttcgag ggcgcggccg aggccgatct ggttgacgct
     5041 ctgcgcgtga acgctcatcc atacgtctcg ctcgtggccg ttgaagaggg cggcgaagtc
     5101 gccggccaca tcttctacag cccggtgacg gtcgagccgg aggcaggcga acttttattg
     5161 atgggactcg ggccgatggc cgtgctgccg cgtgtgcaga agagcggcgt cggctcgcga
     5221 cttgtcaaag aaggtcttga tgaatgtcgt cgtctgggtt gcgcggcggt cgtcgtgctc
     5281 gggcatccaa cctactatcc gcgcttcggg tttgcgccgg cctcgcggct ggggctgcgc
     5341 tgctcgtatg aggtgcccga cgaggtcttc atggcgatgg aacttgagcc gggcgcgctg
     5401 gacgggcggc gcggcctcgt taaatatcac cccgcgttcg acgccgtctg aatgccttcg
     5461 agggcggggg aacacggatg agacggattg aaacggatga gcacggcgtc gtgtaaatcc
     5521 gtggcatccg tctcatccgt gttctattat tcctgacggg aacacatatg ctcgccgtcc
     5581 tcaacatcac gaacttcgct ttgatcgacg acctgagtgt cgagttcgag cgcggcttga
     5641 acctgctcac cggcgagacc ggctcgggca agtcgatcct cgtggacgcg ctcggcgtgc
     5701 tcatcggcgg gcgcttctcc tccgagatgg tcaaggccgg ggcggcgcaa gcttctgtcg
     5761 aggggctgtt cagtgtcggc gagaacccgc gcgtcgagga actactcggc gcggccggtc
     5821 tcgacgcgcg gcccgacggc ggggcggccg agcttgtcat ccggcgcgaa ctcgccgccg
     5881 gcggccgtgg ccgcatcttc atcaacaacc gcctcgccac gctcgccctg ctgcgcgagc
     5941 tgcgtccctt cctcgtggac atccacgggc agggcgacca gcagacgctc ttcaaccccg
     6001 acacgcacct cgaactcctc gacgccttcg gcggccacga ccccgcgcgc cgcgaggtgt
     6061 ctaagctcta cgcgctctgg gccgcgctca atcgcgaact cgaagacctg cgccgcgacg
     6121 acgccgaaaa acttcagctc atcgacgccc tgcgcttcca gaccgaggag cttgaggccg
     6181 cgcgtctgtc gtccggcgag gaggaacagc ttgcctccga gcgccgcctg ctcggcaacc
     6241 ttgagaagat ttccgcgctc tgcgccgacg cctacgacaa gacctacgag cgcgacgact
     6301 cgacggtcgc tcgtctcagc cagctcaccc atcaggtcga agagcttgcg ggctacgaac
     6361 agtcgtacag agactatctc gaaggcgtcc gctcggcgcg cgtcctgctc gaagacctcg
     6421 gcctgtcttt gcgctccttc gccgaccatc tgaagttctc gcccgaaagg ctcgcggaga
     6481 tcgagtcgag gctggcggag gtcgcccgtc tcaagcgcaa gtacggcggc acggtcgagc
     6541 aggccgtcga acacctcgcg cgttcgcgcg agcgcctcga ccaccttgag cgctccgacg
     6601 agcgcgcggc cgaggtgacg aaggaactca cggcggcgcg cgccacatac ctcgccgccg
     6661 cgcgcaagct ccacgacgcg cgcgtgaaga cggcccgcgc gtttggccgc gccgtcgaac
     6721 gcgcgctgac ggaggtggcg atggagagcg cgaagttcgc cgccgagttt gagacgccgg
     6781 gcgccggggc ggacgagcgc gcgctcacca agagcttcac ggccgccggc atcgaccgcg
     6841 tcgagctgca cttctcggcg aatccgggcg agcctccgcg cccgctcgcg cgcgtcgcct
     6901 cgggcggcga ggcgtcgcgt ctgatgctcg tgctgaagac gatgatcaac ccgtcgcgct
     6961 tcccccgcac catcgtcttc gacgagatcg acgccggcat cggcgggcgc gtctccgagg
     7021 ccgtcggtct caagctgaag acgctggccg gcgccaatca ggtgctctgc gtgacgcatc
     7081 aggcgcaggt cgcccgcttc gccgacacgc acctgctcgt caccaaaacc gctgaacgcg
     7141 gcgcgacctc ggtcggcgtc cgcaagctcg acgggcgcgg ccgcgtcgaa gagatcgccc
     7201 gcatgctcac cggcgcgcag atcaccgaga cggcccgccg tcacgcccgc gagatgctca
     7261 aaacggcata aaaacaatgg gggtaagggg taaagggtaa aggggtaagg gaaagacaag
     7321 ttcttctccc ttaccccttt acctcttacc cttgggtggc ttgcctattc ggcgacgacg
     7381 gccaccagtt cgtcctcgtt catcggggcg agcagggagg agctggtcac cttgtcgccg
     7441 ccgtcggtct tgatgaggcg cacgccctgc gcgccgcggc ccgtcttgcg aatctcgctg
     7501 gcttcgatgc ggatgagctt gccgtgctgg gtgatgatca tcagctcgga gtcgtcctcg
     7561 acggggaaga cggcgacgac cttgccggtc ttgtcagtcg tcttcatgtt gatgacgccc
     7621 ttgccgccgc gcgactgcaa gcggtaggtc gagatcggcg tctgcttgcc aaagccgcac
     7681 tcggagaccg agagcatgcg ctcgctgtcg tccgaggaga cggcgcagac cgagacgacg
     7741 aagtcgtcct tccgcaggtc gatgccgcgc acgccgcgcg ccgcgcgccc catcgaccgc
     7801 acgtcgtcct cctcgaagcg gatggcgagg ccgttgtgcg tggcgatgaa gatgcgcttt
     7861 gtgccgtcgg tgagaatcac gtcgagcagc tcgtcg
//
LOCUS       DASVES010000464         5641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_283105, whole genome shotgun sequence.
ACCESSION   DASVES010000464 DASVES010000000
VERSION     DASVES010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283105"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF240_19245"
     CDS             complement(<1..407)
                     /locus_tag="VF240_19245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7177402.1"
                     /translation="MDIPQIIAGRFRIECEIGRGGMGTVYRASHLGLERPVAVKVLKK
                     EIAAEPEVAERFMREARTMARLRHPRAAMIFDAGRLADGRPFIVMEYAEGETLADALL
                     CEGPFAPARAVRVAAQICEVLAEAHALGIVHRDL"
     gene            complement(729..1175)
                     /locus_tag="VF240_19250"
     CDS             complement(729..1175)
                     /locus_tag="VF240_19250"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX7177403.1"
                     /translation="MKTTERIFSRPGAAAGTVGALMALQVVLLGVLGSATTESVTFAG
                     RELHWGCSFREWFGIPCPNCGMTRSVLLSLHGQLTEAVSLNPAGPLLVLGILLFSAAM
                     FFLMAYESRHTGTALARARSSITRGSLAYAALMLVVWMGHWVRAIS"
     gene            complement(1175..1879)
                     /locus_tag="VF240_19255"
     CDS             complement(1175..1879)
                     /locus_tag="VF240_19255"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7177404.1"
                     /translation="MRFCVKCGGTIADSSPTPGSWQEPSGNLYDTPGSSSGAGSGGFG
                     QQPGTYAPPPVSYGGGYGGGAAMASTGIGGLPYAEWIDRVLAALIDGGIATGVMIVLY
                     IIFGVIGGVIGGIGSSLNSDAVGAIGSLSCCLGFLLAGLSVFVVGIYNKIFLVSKRGY
                     SIGQGVMKLKVVDAMGNIPQMGTLVVRLLAQVAMGFIPIIGIFLGIANALWPLWDEKK
                     QTLHDKAAGTFVLKTG"
     gene            complement(2172..2996)
                     /locus_tag="VF240_19260"
     CDS             complement(2172..2996)
                     /locus_tag="VF240_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177405.1"
                     /translation="MKVETLKEEAGSPFDAALTGALARRGMSAREVCPPGDALARRVL
                     EEYGAMFLAAETVRVPPRCVFSGEEQVEEFQRRATWRAAEFGADTVELQPVAMAALLE
                     ARREAEAEGLSVTPRGGREAGRRTYADTLRLWDSRCRPAIEHWCARGRLTGEEGEALR
                     CLSLIEQVAAVLALEGRGIFFSKDFTKSILLSVAAPGASQHLSMLAFDAVEFTDARVR
                     LLLARHGWFQTVLSDLPHFTFLGLAEAELPRLGLRRVEEGGQEFWIPDTERSEVRG"
     gene            3249..3620
                     /locus_tag="VF240_19265"
     CDS             3249..3620
                     /locus_tag="VF240_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177406.1"
                     /translation="MSQLQNFREALALYRRHAWRPVRALMRDATLAELRRESPGAEGE
                     ADAAAVFEGVRAEESVVDALWFTRASAGGREAWELRLVAEPPYALFELFEPDEAEEDR
                     EDVRREMEARLREYVAPTPES"
     gene            complement(3652..4734)
                     /locus_tag="VF240_19270"
     CDS             complement(3652..4734)
                     /locus_tag="VF240_19270"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7177407.1"
                     /translation="MQNENRNAGMRPDDAGANADAVACPSCHTMLVRGMRFCRMCGYR
                     MGEGVEEFAETRRFDGTMPPVATSAPPKGVAADAPFQTPNAAPFQTPHAWGAMSPAQP
                     TGATYEPASTFGKLAKTCNPMRLGWVFWVILSIVILTASGAAIRQARRGGAFGGPRVA
                     AVPPARAFLGVDGWDPAPGGGGAMLQGIAAPDTPVERAGLIGGDIIINFDGQAIADDD
                     ELRKVIAATPVGKAVEVVFIRDGATQKTTLTVGSEREYKGWEAIEARPGGRGLFGIDD
                     YDRVRVPGREIYGVRVNDIDRNEPADLAGMQRGDIVIQIGDSPVRTAGDLRYLINKAV
                     PGSVVKVIVVRGPEQVEIPVKVGRSK"
     gene            5146..5436
                     /locus_tag="VF240_19275"
     CDS             5146..5436
                     /locus_tag="VF240_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177408.1"
                     /translation="MEVTKALAALQPLYGKGNVEVVTEDGARVRGTVRSLRTTQALTK
                     PSVKIERADGEVVKIPFNVITEIIDHNNENERREEEEPKGGFFGRRFTLGFF"
BASE COUNT          939 a         1850 c         1956 g          896 t
ORIGIN      
        1 aggtcgcggt ggacgatgcc gagtgcgtgg gcctcggcga gtacttcgca gatttgggcg
       61 gcgacgcgca cggcgcgtgc cggcgcaaac ggcccttcgc acaggagcgc gtcggcgagc
      121 gtctcccctt cggcgtattc catgacgatg aaggggcggc cgtcggcgag gcggccggcg
      181 tcgaagatca tggcggcgcg cgggtgccgc agacgcgcca tcgtccgcgc ctcacgcatg
      241 aagcgttcgg ccacctccgg ctcggcggcg atctctttct ttaagacttt gacggcgacg
      301 gggcgttcaa gcccgaggtg ggaggcgcgg tagaccgtac ccatgccgcc gcgtccgatt
      361 tcacactcga tgcggaaacg gccggcgata atctggggga tatccattgt gaccctctcg
      421 aacgaaagac gaaggggtcg cctgcttggt cgcgctttac atcgctcggg gacggaaggt
      481 cagcgatggg ggctgggggc gctgcctgcg gcggcactgt gaaactttta ggctttaggg
      541 agtttgcaaa ttgcccggcc ccgttgggga gccggacgaa gcagagttta gcacttttgc
      601 cggcgagcgc aaaagtttga gggaaaaatt aagtcgcggc cggcgcgcgc gcgtccggtc
      661 ccgcatacag cgaaggccgc ccccgcgggc ggcccccact cgattcacct tcgggccgcg
      721 gccggcgttt acgagattgc gcgcacccag tggcccatcc acacgactaa catcagcgcg
      781 gcgtaggcga gggagccgcg cgtgatcgaa gagcgcgccc gcgcgagcgc cgtgcccgtg
      841 tgcctgctct cgtaggccat caggaagaac atcgcggcgc tgaagagcag gatgccgagc
      901 acgaggagcg gccccgccgg attgagcgac acggcctcgg tgagctggcc gtgcagactg
      961 agcaggacgc tgcgcgtcat gccgcagttc gggcacggga tgccgaacca ctcccggaac
     1021 gagcagcccc agtgtaactc ccggccggcg aaggttaccg actcggtcgt ggccgagccg
     1081 agcacgccga gcaggacgac ctgcaacgcc atgagcgcgc cgaccgtccc ggccgccgcg
     1141 cccggccggg agaaaattct ctccgtcgtc ttcatcagcc ggtcttcaga acgaaggtgc
     1201 ccgccgcctt gtcgtgcagc gtctgtttct tctcatccca gagcggccag agcgcgttgg
     1261 cgatgccgag gaagatgccg atgatcggga tgaagcccat ggcgacctgc gcgagcaggc
     1321 ggacgacgag cgtgcccatc tgcgggatgt tgcccatcgc gtccacgacc ttcagcttca
     1381 tgacgccctg gccgatggag tagccgcgct tgctgacgag gaagattttg ttgtagatgc
     1441 cgacgacgaa gactgacagg cccgccagca aaaaccctaa gcagcaggag agcgagccga
     1501 tggcgccgac ggcgtcgctg ttcaagctcg acccgatgcc gccgatgacg ccgccgatga
     1561 cgccgaagat gatgtagagc acgatcatca cgcccgtggc gatgccgccg tcgatgagcg
     1621 cggcgagcac gcggtcgatc cactcggcgt aagggagtcc gccgattccg gtggaggcca
     1681 tcgccgcgcc gccgccgtag ccgccgccgt agctcaccgg gggcggcgcg taggtgccgg
     1741 gctgctggcc gaagccgccg ctgccggcgc cgctgctgct gcccggcgtg tcgtagaggt
     1801 tgccgctagg ctcctgccag gagccggggg tgggggacga atccgcaatc gtgccgccgc
     1861 atttgacgca gaacctcatg ccgtcggcat tctcggttcc gcaggacggg cataaaatca
     1921 tattcactct ccttgtgacg atttttttga cgcgggccgc cgggcgcacg tcaggcgtcc
     1981 tcgttggcgg tcacgtttgt gaagtgtttg ggtcaggccg gacaaaaccc actcaggtca
     2041 aacgcagacg ttctcaaagc acagacgaac ggagacaagt ctttctcaca aaagacggga
     2101 aaaagcaaga aagaaaaaga ggttagaggt tagaggtcag aggttagtgg aagacagttt
     2161 tttaccgacc tctaacctct gacctctgac ctctccgtgt ctggtatcca gaactcctgc
     2221 ccgccctcct cgacgcgccg gaggccgaga cgcggcagct ctgcttcggc gaggccgagg
     2281 aaggtgaagt ggggcaggtc gctcagaacg gtctggaacc agccgtggcg cgcgagcaga
     2341 aggcggacgc gcgcgtccgt gaactcgacg gcgtcgaagg ccagcatcga caggtgctgc
     2401 gaggcgccgg gcgcggcgac cgaaagaagg atgctcttgg tgaagtcctt gctgaagaag
     2461 atgccgcgcc cttcgagcgc gagcaccgcc gccacctgtt cgatgagcga cagacagcgc
     2521 agggcctcgc cctcctcgcc cgtgagccgc ccgcgcgcgc accagtgttc gatggcgggc
     2581 cggcaacgcg agtcccagag tcggagcgtg tcggcgtagg tgcgccgccc ggcctcgcgc
     2641 ccgccgcgcg gcgtgacgct caggccctcg gcctcggcct cgcggcgcgc ttccagcaaa
     2701 gccgccatcg ccacgggctg caactcgact gtgtccgcgc cgaactccgc cgcgcgccac
     2761 gtcgcccggc gctgaaactc ctcgacctgc tcctcacccg agaacacaca gcgcggcggc
     2821 acgcgcaccg tctcggcggc cagaaacatc gcgccgtact cttcgagcac gcggcgcgcg
     2881 agcgcgtcgc cgggcgggca cacctcgcgg gcgctcatgc cgcgtcgcgc gagcgccccc
     2941 gtgagcgccg cgtcgaaagg tgagccggct tcttccttca gcgtttctac tttcaagcgg
     3001 gttgcgattt ctgcaaatgg ctttgacgtg gtcggaactc gtagacgcat gataaaagcc
     3061 gcgaccgagc cgggtcaacg accgcgctgt tcccctccgg ttttcgccgc cgcgccgacg
     3121 acgcttcctc ggcttttact tcgcgcgtcg tctggagcgc ggttgccgcg agtcgccgtg
     3181 cctgatgtat gcttgatgcg acggcggcgt aaggtgagcg cgttcggccg tccgtcggcg
     3241 aggggacgat gagtcagcta caaaactttc gagaggcgct ggcgttgtac cgccggcacg
     3301 cttggcggcc ggtacgcgct ctgatgcgcg acgcgacgct cgcggagctg cgtcgcgagt
     3361 cgccgggcgc cgagggcgag gcggacgctg cggcggtgtt cgagggcgtt cgggcggagg
     3421 agtctgtggt ggacgcgttg tggttcacgc gcgcgtcggc cggcgggcga gaggcgtggg
     3481 agctgcggct ggtggccgag ccgccctacg cgctcttcga gttgttcgag ccggacgaag
     3541 cggaggaaga ccgcgaggac gtgcggcggg agatggaggc gcgtctgcgc gagtatgttg
     3601 ccccgacgcc ggagagttga acccatttgc cggcgccggg gaagccccga ctcacttgct
     3661 gcggccgacc ttgacgggta tctccacctg ctcggggccg cgcacgacga tcactttgac
     3721 gacgctgccg ggcacggcct tgttgatgag gtagcgcagg tcgcccgcgg tgcggacggg
     3781 cgagtcgcct atctggatga cgatgtcgcc gcgctgcatg ccggcgaggt cggccggctc
     3841 gttgcggtcg atgtcgttga cgcgcacgcc gtaaatctcg cggccgggca cgcgcacgcg
     3901 gtcgtagtcg tcgatgccga acaggcccct gccgccgggc cgggcttcga tggcctccca
     3961 ccccttgtac tcgcgctcgg agccgacggt cagcgtcgtc ttctgcgtcg cgccgtcgcg
     4021 gatgaagacg acttcgacgg ccttgccgac gggcgtggcg gcgatgacct tgcgtagctc
     4081 gtcgtcgtcg gcgatggcct gaccgtcgaa gttgatgatg atgtcgccgc cgatgaggcc
     4141 ggcccgctcg acgggcgtgt cgggcgcggc gatgccttgg agcatggcgc cgccgccgcc
     4201 cggcgccggg tcccagccgt caacgccgag gaaggcgcgc gccgggggca cggccgcgac
     4261 gcgcgggccg ccgaaggccc cgccccgccg ggcctgcctg atggcggcgc cgctggccgt
     4321 caggataacg atggagagga tcacccagaa gacccagccg aggcgcatgg ggttacaggt
     4381 cttcgcgagt ttgccgaaag tgctggccgg ctcgtatgtc gctcccgtcg gctgcgcggg
     4441 cgacatcgcg ccccacgcgt gcggcgtctg gaagggagcg gcgttcggag tctggaaggg
     4501 cgcgtcggcg gcgacgcctt tcggcggcgc gctcgtcgca acgggcggca ttgtgccgtc
     4561 gaagcgacgc gtctcggcga actcctcgac gccctcgccc atccggtagc cgcacatgcg
     4621 gcagaagcgc atgccgcgca cgagcatcgt gtggcaggag gggcaggcga cggcgtcggc
     4681 gttggccccc gcgtcgtccg gccgcatccc ggcgtttctg ttttcgttct gcatggcctt
     4741 actgtctgtc gaaaagggaa gtgtcgtcag tccccaagga cttccgacaa gcgcgctcgc
     4801 tagtgcgtct accgccgtct cggttatacg cctcctggag cctcgcagtt gcaccgcgtc
     4861 ctgcggcgct aactttaacc gagaaacacc ggcgcgcgaa agccccgtga tgcacgccgc
     4921 aacgccgaag gtaatgcgcg cttggcaggg gcgcggccgt ccgttggcac gggttatgct
     4981 tggtacgctc gacgaaagtc ggatttttag gccccgcggg gcaccaaaag cggcggcggc
     5041 gcgcatctga agtgccgaaa cagtccgcaa cagacagccc acagtgtttt atcaccggac
     5101 ataccccgcc gccggggagg ccggggaagg aacggtccca aacaggtgga agtcactaaa
     5161 gcactcgcgg cgcttcagcc gctctacggt aaaggcaacg tcgaggtcgt cacggaagac
     5221 ggcgcgcgcg tgcgcggcac cgtccgttct ttgcgtacca cgcaggcgct caccaagccg
     5281 agcgtcaaga tcgagcgggc cgacggcgag gtcgtcaaaa tccccttcaa cgtcatcacc
     5341 gagatcatcg accacaacaa cgagaacgaa cgccgcgaag aggaagaacc caagggcggc
     5401 ttcttcggtc ggcgcttcac gctcggcttc ttctaaagcc tcggcccgcg gccgatccaa
     5461 gaccttcagt gttcgacctc tccaacagca tcgagtggcg gccgacgcgc caccgcggca
     5521 tccacctccg cgtgctgcgg cgcgacgagc gaagcggcga cgccaccgtc ctcatccgca
     5581 tggagccggg ctgctcctac ccggcccacc gccaccgggg gctggaagaa gtgctcatcc
     5641 t
//
LOCUS       DASVES010000465         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_560518, whole genome shotgun sequence.
ACCESSION   DASVES010000465 DASVES010000000
VERSION     DASVES010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560518"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1167)
                     /locus_tag="VF240_19280"
     CDS             complement(169..1167)
                     /locus_tag="VF240_19280"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX7177409.1"
                     /translation="MRLRTRLGVLLSVIFGVVATLTAAVAPAAQDAAEGWRGERRGDA
                     GKDLNTIFFVDRKRGWAAGDGGVIVRTEDEGRTWRRQSVGTSEAVNDIYFLDKEDGYL
                     VAGNRIYVSADAGVTWRESANFPASTFGGAEPELYSVRFTSKKRGWIVGSVSRRDTVV
                     DSLVLKTTDGGASWQRFRVPTKSELINLDFSGDKRGWIVGNSGVVLHTRDAGETWTLQ
                     RSGTASTLYSVEFRGDKDGWAVGQGGTIIKTRDGGETWRVVESPVRTTLLGVRFANDE
                     EGWVVGRGGVILRTADEGNTWLQQESRTKRNLFALFVDKDNCWAVGGDGLVLQYDR"
     gene            complement(1426..3711)
                     /locus_tag="VF240_19285"
     CDS             complement(1426..3711)
                     /locus_tag="VF240_19285"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HEX7177410.1"
                     /translation="MRNGRMLALLAFGLLIIFFAGADTTARAQGIIVPRPCVRCPRPP
                     RPIPQLPPALPVKSIKIDTKVAAQVATTHVEQVFRNDTDATLEGTYFFPIPESASISE
                     FAIWDGDQRLVGEVRSREEARRIYDEIVRSQRDPGLLEYAGRDLFQASIFPIPPHSDK
                     KLELTYTQVLKADSGTVSYRYPLGTGRNASAVVGAVSGRVELQGREPLRNIYSPTHDI
                     DISRGQEERRAVVSFESKVGQEPQDFQLFYTLTNADFGLTLLTHREPGKHGYFLLTIS
                     PKDKLREEEYAAKDIVFVLDTSGSMAAEGKMEKARAALLFGVRSLRDGDRFNVINFAG
                     EEHLMSGGLIAADGAGRARGEEFVKALRPLGGTNINAALLAGMRQFEQSERPRMLVFM
                     TDGLPTVGETNAQRIVDNARQARVAGLRVFTFGVGYDVNTPLLDKIAAENGGVADYID
                     PKEDIEVKVSNFFSKVSHPVLTAMHLEMGGVETDLVYPREMPDLFRGAQVTIIGRYRN
                     SSDVRGAVLRLTGRAGNESRAYPYEKLNFPSESADNDFLPRLWATRRVGWLMEQVRTN
                     GESQELRDEIVDLGTRYGIVTPYTSYLALESSEATRFITSNTAPDNRPMTTTGGRGSG
                     ALARGEASKRSPASPMSAKDGAARAISGADAVRESRRARTQQEAVRLDDDESIRTEND
                     PRSSRRRVGSKTFYLRDGVWLDAEFKEDARLPETALIFGDEEYFALIKREPQLARFFA
                     LGERVVVVFKGHVYRVQAAKP"
BASE COUNT          619 a         1326 c         1326 g          689 t
ORIGIN      
        1 acgcggagtc ttgacgcttc ttctccgcgc cccctccgcg ccctttgcga ctccgcggtg
       61 aatctatttt cgcctcactc atgtgaacct cgctctaaac tgacgctgaa atgcccgcac
      121 gcccttgcga atcgctataa atcgcagcac ccccgccagc ccgccctctt acctgtcgta
      181 ctgcaagacg aggccgtcgc cgcccacggc ccagcagttg tccttatcca cgaagagcgc
      241 gaagaggttg cgcttggtgc ggctctcctg ctgcaaccag gtgttgccct cgtccgcggt
      301 gcgcaggatg acgccgccgc gcccgacgac ccagccctcc tcgtcgttgg cgaacctcac
      361 tccgagcagc gtcgtcctga ccggcgattc gacgacgcgc cacgtctcgc cgccgtcgcg
      421 ggtcttgatg atcgtcccac cctgaccgac cgcccaccca tccttgtcgc cgcggaactc
      481 gacgctgtag agcgtcgagg ccgtgcccga acgttgcagc gtccacgtct cgcccgcgtc
      541 gcgcgtgtgc aggacgacgc ccgagttgcc gacgatccag ccgcgcttgt cgccggagaa
      601 gtcgaggttg atcagctcac tttttgtcgg gacgcggaaa cgctgccagg aggctccgcc
      661 gtcggtcgtc ttgaggacga ggctgtccac gaccgtgtcg cggcgcgaga cgctgccgac
      721 gatccagccg cgcttcttgc tcgtgaagcg cacgctgtag agttccggct cggccccgcc
      781 gaaggtggag gccgggaagt tggccgactc gcgccaggtc acgcccgcgt cggccgacac
      841 gtagatgcga ttgccggcga cgaggtagcc gtcctccttg tcgaggaagt agatgtcgtt
      901 gacggcctcg ctcgtgccga ccgactgccg gcgccacgtg cgcccctcgt cctccgtgcg
      961 gacgatcacg ccgccgtcgc cggccgccca cccgcgcttc cggtcaacga aaaagatcgt
     1021 gttcaaatcc ttgccggcgt cgccgcgccg ctcaccgcgc cagccctcgg ccgcatcctg
     1081 cgcggccggc gcaacggcgg cggtgagcgt ggcgaccacg ccgaagatga cagatagcaa
     1141 cacgcccaac ctcgtcctca gtctcatgcg gcacaccttc atcgttcgcg cgacggaaat
     1201 cggtttgatt gattgctctc aggtttgtac acgaaccgtg gccggagaga caagcccgca
     1261 cgcggcgcgc tgtgagaaac tgctcaacca gtcgcgcgag cggcggcagc caacaggcag
     1321 tagcccgacc gtgagggagg gcgtactcac gtcggagcac gccctccctc acggtcgggc
     1381 tactgcctgt ctgacaggcg gggctatttc atcgcgctta cctcgtcacg gcttcgccgc
     1441 ctgcacgcgg tagacgtgac ccttgaagac gacgaccacg cgctcgccga gggcgaagaa
     1501 gcgcgcgagc tgcggctccc gcttgatgag ggcgaaatat tcctcgtcgc cgaagatgag
     1561 cgccgtctcg ggcaggcgcg cgtcctcttt gaactccgcg tccaaccaca cgccgtcgcg
     1621 caggtagaag gtcttgctcc cgacccggcg gcgcgaggag cgcgggtcgt tctccgtccg
     1681 aatgctctcg tcgtcgtcga ggcggacggc ctcctgctgc gtgcgcgcgc gccggctctc
     1741 gcggacggcg tccgcgcccg agatggccct cgccgccccg tccttcgccg acatcggcga
     1801 ggcgggcgac ctcttggaag cctcgccccg cgcgagcgcg cccgaccccc tgccgcccgt
     1861 ggtggtcatc ggccggttgt ccggcgcggt gttgctcgtg atgaaccttg tcgcctccga
     1921 cgattcgagc gcgaggtaag aggtgtaggg cgtgacgatg ccgtagcgcg tcccgagatc
     1981 gacgatctcg tcgcgcaatt cctgcgactc gccgttggtg cgtacctgct ccatcaacca
     2041 tccaacacgg cgcgtcgccc acaggcgcgg caggaagtcg ttgtcggccg actccgaggg
     2101 aaagttgagc ttctcgtagg ggtaagcgcg cgactcgttg ccggcccttc ccgtgagacg
     2161 caggacggcg ccgcgcacgt ccgacgagtt gcggtagcgg ccgatgatcg tgacctgcgc
     2221 gccgcggaag aggtcgggca tctcgcgcgg gtaaacgagg tcggtctcga cgccgcccat
     2281 ctccagatgc atggcggtga ggacgggatg actcaccttc gagaaaaagt tggagacctt
     2341 gacctcgatg tcctccttcg gatcgatgta gtcggcgacg cctccattct cggccgcgat
     2401 cttgtcgagg agcggcgtgt tcacgtcgta gccgacgccg aaggtgaaga cgcgaaggcc
     2461 cgcgacgcgc gcctgacgcg cgttatcgac gatgcgttgc gcgttcgtct cgcccaccgt
     2521 gggcagcccg tccgtcatga agacgagcat gcgggggcgc tcactctgct cgaactgtcg
     2581 catcccggcg aggagcgccg cgttgatgtt cgtcccgccc aaggggcgca gggctttgac
     2641 gaactcttcg ccgcgggcgc ggcccgcgcc gtcggccgcg atgaggccgc cgctcatcag
     2701 gtgttcctcg cccgcgaagt tgatgacgtt gaagcggtcg ccgtcgcgca aagagcggac
     2761 gccgaagagg agcgccgcgc gcgctttctc catcttgccc tcggcggcca tcgagccgga
     2821 ggtgtctaag acgaagacga tgtctttggc cgcatactcc tcctcgcgca gcttgtcctt
     2881 cggcgagatg gtcaggagga agtagccgtg cttgcccggc tcgcggtgcg tgagcagggt
     2941 gagtccgaag tcggcattgg tgagcgtgta gaagagttga aagtcctgtg gctcctgacc
     3001 caccttcgac tcgaagctga cgacggcgcg gcgctcctcc tgtccgcgcg agatgtcgat
     3061 gtcgtgcgtc ggcgagtaga tgttgcgcag cggctcgcgg ccctgtagct cgacgcggcc
     3121 cgagacggcg ccgaccacgg ccgaggcgtt gcgccccgtg ccgagcggat agcgatagga
     3181 gaccgtgccc gagtcggcct tgagcacctg agtgtaggtc agctcaagtt tcttgtcgga
     3241 gtgcgggggg atggggaaga tgctggcctg gaagaggtcg cggccggcgt attccaaaag
     3301 accggggtcg cgctggctgc ggacgatctc gtcgtagatg cggcgggcct cctcgcgcga
     3361 gcgcacctcg ccgacgaggc gctggtcgcc gtcccagatg gcgaactcgg agatggaggc
     3421 cgactcgggg atggggaaga agtacgtgcc ttcgagcgtg gcgtcggtgt cgttgcggaa
     3481 gacctgctcg acgtgcgtgg tggctacctg agcggcgacc ttcgtgtcga tcttgatgga
     3541 cttgacgggg agcgcgggcg gaagctgggg gatggggcgc ggcgggcgcg ggcaacgcac
     3601 gcaggggcgc ggcacgatga tgccctgcgc gcgggccgtc gtatcggctc ccgcaaaaaa
     3661 gattatcagt agaccgaagg cgagcagcgc cagcattctt ccgtttctca tcttcttcac
     3721 ctcaaagcgg tctgcgattc ttaagacctt cgccgcgaac gccgaaaaaa cggacgccgc
     3781 cgcggcttcg ttggtagaac gccccggccg ctacttcttg cagggccgct gcggggcgga
     3841 gtttatcacc gatggaggcg tttttcctcg ggagcgcgga tatcctgccc gctatgagcg
     3901 cggagcgaac agtaccgcga gcggaagcct cgcgggtgcg gccgcgtgcg ccgcacccgc
//
LOCUS       DASVES010000466        11796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_671578, whole genome shotgun sequence.
ACCESSION   DASVES010000466 DASVES010000000
VERSION     DASVES010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11796
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671578"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VF240_19290"
     CDS             <1..378
                     /locus_tag="VF240_19290"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7177411.1"
                     /translation="FWQTAERRPAFPRFYKQFLALRRSSAALRRGETEWLKNSDESRV
                     LTYARRYADEEIVVAINFSSQPFAGTVETRGGPFDEITPDILPPLPADATPQQRAARL
                     RTTGLPALSLEAWGYRVYRRPVK"
     gene            complement(497..1846)
                     /locus_tag="VF240_19295"
     CDS             complement(497..1846)
                     /locus_tag="VF240_19295"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX7177412.1"
                     /translation="MKIITHRAGAPRLVLHTLTLLLLVWSVPSPLAFAQRPAPPPQPY
                     RLTAEDEAFLEDLQRRSFQYFYDHADPSTGLVLDRARADGTRHDASHPSHRIASSAAT
                     GFGLSALCIAAERGWIPRREARERVVKTLRFYDERAAHMKGWFLHWMDAPTGERRWQS
                     EYSSIDTALLLAGVLTARGYFQNDAEVRRLATRVYERVNFPWMLARHPTLLSHGWKPE
                     TGFLKPRWDTYSEHLILQLLAIGSPAHPITPRAWLAWRRERITYGGYTYITGGPLFVH
                     QYSHAWADFRGRREGWYPHTDYFENSVAATRAHRQFCIDLAPEFPGYTADVWGITASD
                     SARGYTAWGGPPRHPDIDGTVVPAAAGGSLMFTPDISLRALREMKERYGERIYGRYAF
                     IDAFNPTTGWTNPDVIGIDIGPVLLSAENLRSGNVWRWFMRNGEIPRAMRSVGLRRQ"
     gene            complement(1971..4190)
                     /locus_tag="VF240_19300"
     CDS             complement(1971..4190)
                     /locus_tag="VF240_19300"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7177413.1"
                     /translation="MNHAQRTSLALVLVLITVTTPVTQAQRARRDLERRIDALLARMT
                     TEEKLGQLQQLDGHADGRAKDDQPALVRAGRLGSTLNVRGVKNTNALQRIALDESRLK
                     IPLIFGFDVIHGYRTIFPVPLAEASSWDPAAVERAAAVAAKEARAAGVHWTFAPMLDI
                     ARDARWGRIVEGSGEDPFLGAAMGRARVRGFQGADYSAPERVVACAKHWVAYGAAEAG
                     RDYNTTEVSETTLREIYFPPFKAAVEAGVGTFMSAFNDLNGVPTSANPFTLTKVLRGE
                     WRFDGFVVSDYTSVEELLKHGVAADGREAAQLALNAGVEMEMVSRLYNTHGAELLRGG
                     KLTPARLDEAVRRILRIKFRLGLFDSPFADEAREREALLNPAHLAAAREVASRSMVLL
                     KNERNILPFAKNIRSLALIGALADDQANMLGSWTGDGRKEDVVTLLAALREKLPRADI
                     KHVPGCDILCASSDNFDEAARAARAADAVIVVVGESAEMSGEAASRTSLDLPGRQMDL
                     IKAVHAAGKPYAVVLVNGRPLTINWAAENSPAILETWFAGTQAGPAIADVLFGDVNPG
                     GKLPVTFPRAVGQLPLYYNHKNTGRPPDANNKYTSKFLDVPWTPLYPFGHGLSYTKFQ
                     LSNLQLSARRITRGASLRLSVELENTGRRAGDEVVQLYLRDVASSVTRPVRELKGFER
                     VALRPGERRRVEFTLSPAHLGFYDREMRFVVEPGAFKVFVGTSSVGGLEADFEVVER"
     gene            4337..5782
                     /locus_tag="VF240_19305"
     CDS             4337..5782
                     /locus_tag="VF240_19305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177414.1"
                     /translation="MNTQRGTTIHENTRCGFGPCRAAPRRCGPKTHTRLLLKTLLASL
                     ILLASPSNAPAQGRQRKTPPAGERSEALSETVATLLRDALELLRAGKHAEAEPLVRRV
                     IAVQPSSADAHGLLGLILDQGGRTHEAEREYRAALRLNPRSVSARANLGVLLARGLRH
                     AEALRLFESVLADAPGHPQATLNLGAALFHLRRFDEAERALSTASGLVPQAAEPHYYL
                     GQIAASRGRGEEAAEHWLRALALRENFPEANFALAEELRRQRRYEGAAEFYGRALAGD
                     SKKPVHHIRLGGVLMLLVRYQEALAVFRRAVEAFPMSAEAHYFAGIAARGLGDYEAAE
                     ASLRKSLLLRPDSVDALAQTGFVVGERGRTHEAERILRRAVALDRRHFYAHYDLGRLL
                     VRERRYEEAIPVLRSSTGIRDHDPGVHYQLFIAYSRLKRTDEAARELSIFKRLEEERK
                     ARRSAGGEEQVEDTLPRPATAEPAKSEDEPD"
     gene            complement(5948..9433)
                     /locus_tag="VF240_19310"
     CDS             complement(5948..9433)
                     /locus_tag="VF240_19310"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7177415.1"
                     /translation="MKKTPSVIRRAPVLALLLLCFAATAQAQFRAGVQGTVSDPAGAV
                     VTGASVTLTNTETNRAQQTTSSDEGFYSFSNLAPGRYTLTAEKAGFKKTFFDNVTVSA
                     ESVQGMDVVLTTGEVAETVTVTSEAAQGLETENANVSRAITTEEVRRLPQFGRDPYEL
                     LRLTPGVFGSGARSGVGESVKLPNSTGPGGSSNSIFQAENQPQITANGQRISANNFQI
                     DGVSVNSLQFGGAAVVTPNQEAVKEVRVVSSAYSAEYGRNSGAQVQVVSQNGTNTFHG
                     SLFLKANDPRWNAFNKYGPDFNRAGPERVSSRFKQFGGSFGGPLPLPRFGEGGPSTIG
                     GRDKSFFFFSYEGLRSHSSGSAVKFVETPEYRQQIIAARPNGIVARILTSQGIQPRIV
                     SVLSQNCAQFNNDPSRCRVVGNGLDIGSPTGATGQYISTGNAVGGGFDGIPDIQRALI
                     ATPRDINSNQYNLRLDFTPTERDQIAISTFLTRNDILGSDEGSGGRPSSDIRSTPFNS
                     SGAITYIRTLSNTMVNELRFNATRFALNEVESAVDTNFGIPRIEIEGLPFDRIRFGAP
                     QGETSPGIFAQNTFELRDTLNVVRGSHNFKFGGELRWEQDNNNLNGGSRPLFTFSGLF
                     NFANDTPLFYQINADPRTGGPADAQRYFRSKYYGFFVQDDWKARPNLTLNIGLRYELF
                     TPLKEKNGQLSNLVFGSNGLQNARLEVTDELFKADRNNFAPRLGFAWSPNYGDSFLGL
                     LRENRAVIRGGFGISYNRTPNVLFSNSRGNPPFFARYQICCGTAGPAGGDSFSTPFAD
                     GQILYALGSSNSPFSYPTNPALAVGIDPATGSARGRSVEVYGSEPDIPNAYVYTYSLE
                     TQLELPGKFTAELGYQGSSGHKLIRLVNQNFLYPNNPAFFAVFFPQPDVNSNYNGLNA
                     RLVRRFSAGTRFDVYYRFSKSIDTLSNEGPGAESNQTFPQDLRTERGPSDFDVKHYLV
                     TSGVWELPFFRGRRDTLGKVLGGFQLSGIMTWHTGFPWTPKTGECTRTPGGPTLCPAR
                     PARYFGGALEDTGNDAFIRPGGNFPGIQTNGGPYFDINNPGGVLPPGIGRNSFRGPRY
                     FSLDLTVGKRTGLPAFMGDGSFLELRANLFNALNNLNLSPLRFFAPNIENPDFGRSER
                     GLAGRVVELQARISF"
     gene            9932..11092
                     /locus_tag="VF240_19315"
     CDS             9932..11092
                     /locus_tag="VF240_19315"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7177416.1"
                     /translation="MFLLAAACALSVAGACVQPTEQGGAAGGAQNRAPKKAGEKVKIG
                     FSMDTLKEERWQRDKEHVEQYAREAGADLEVQVANGDDAAQTKQCETMLTKGVDVLIV
                     APHNGVAAAAIVQMAKQRGVPVISYDRLIRNSDVDLYVSHQVEKMGEMQGQYAFEKVP
                     RGNYVLIGGAQTDNNALLLRKGQLNVLQPAIDRGDIKVISDLYARDWLASEAQRITED
                     ALTRAGGKVDAIVASNDGTAGGAIAGLPPALVGKVLVTGQDAALDGIQRIVEGKQTMT
                     IYKPIKPLAKSAVDAAVQLARGEALSAPDRINNGKIDVPSILHAPQAVDKANVMQTVI
                     ADGYHKFDDVYKNVPPDQRPKQTTAAAGVAPTFVFPLLFLGAALFAGVVLKR"
     gene            11089..>11796
                     /locus_tag="VF240_19320"
     CDS             11089..>11796
                     /locus_tag="VF240_19320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7177417.1"
                     /translation="MSDAPYPYVPLLQMRDITKTFPGVRALDGVSFDLHPGEVHALVG
                     ENGAGKSTLMKVLGGVYPHPQYGGEFSIDGYERRFGGVRDAEAAGIAVIFQEMSLVKE
                     LSIAENIFLGREPRRFGIIRWEELYSRAQKLLADLHLEADARTPVGNLGIGQQQLVEI
                     AKALSQEARILVLDEPTAALTDAEVETLFRIIAKLKARGVGMVYISHKLDEVFRISER
                     ITVLRDGRTVGTDRAADL"
BASE COUNT         1999 a         3714 c         4108 g         1975 t
ORIGIN      
        1 ttctggcaga cggccgagcg ccgccccgcc ttcccgcgct tctataaaca gttcctcgcg
       61 ctccgcaggt cgtccgcggc cctgcgccgg ggcgagaccg agtggctgaa aaactccgac
      121 gagtcgcgcg tcctcactta cgcgcggcgc tacgcggacg aggaaatcgt cgtcgccatc
      181 aacttctcct cgcagccctt cgccggcacg gtcgagacgc gcggcggccc gttcgacgag
      241 atcacccccg acatcctgcc gccgctcccg gccgacgcca cgcctcagca gcgcgccgca
      301 cgactgcgga cgacgggact ccccgccctc tcactcgaag cttggggcta ccgcgtatac
      361 cgccgccccg tgaagtgagc cgtcgcgaaa ggttgggagt agaacacgga taaaacggat
      421 gaagacggat tagcacgaat ccgtcttcat ccgttttatc cgtcttatcc gtgttcttct
      481 ggcccttttc tatcatctac tgccggcgca gcccgaccga acgcatcgcg cgcggtatct
      541 cgccgttgcg catgaaccag cgccagacgt tcccgctgcg aaggttctcc gcgctcagga
      601 ggacggggcc gatgtcgatg ccgatgacgt ccgggttcgt ccacccggtc gtcgggttga
      661 aggcatcgat gaacgcgtag cgcccgtaaa tccgttcgcc gtatctttcc ttcatctcac
      721 gcagcgcgcg cagagagatg tcgggcgtga acatcaggga gccgcccgcg gctgccggga
      781 caaccgtgcc gtcgatgtct gggtgacgcg gcggcccgcc ccaggccgtg tacccgcgtg
      841 cgctgtccga cgccgtgatg ccccacacgt ccgccgtgta gccgggaaac tccggcgcga
      901 ggtcgatgca aaactgcctg tgcgcgcgcg tcgcggcgac ggagttttcg aaatagtctg
      961 tgtgcggata ccagccctca cgacggccgc ggaagtcggc ccacgcgtgc gagtactggt
     1021 ggacgaagag cgggccgccg gtgatgtatg tgtaaccgcc gtaggtgatg cgctcgcgcc
     1081 gccacgcgag ccatgcgcgc ggcgtgatgg gatgtgccgg cgaaccgatg gcgagaagtt
     1141 ggagaatgag gtgctcgctg taggtgtccc aacgcggttt gaggaagccg gtctcgggct
     1201 tccagccgtg cgagagtagg gtggggtggc gggcgagcat ccacgggaag ttgacgcgct
     1261 cgtagacgcg tgtcgcgaga cgcctgacct cggcgtcgtt ctggaaatag ccgcgcgccg
     1321 tgagcacgcc ggcgagcagg agcgccgtgt cgatggacga atactcgctc tgccagcgac
     1381 gctcgccggt cggagcgtcc atccagtgca ggaaccagcc cttcatgtgc gccgcgcgct
     1441 cgtcgtagaa acgcagggtc ttgacgacgc gctcgcgtgc ctcgcgccgc ggtatccagc
     1501 cgcgctcggc ggcgatgcag agcgcgctca ggccgaagcc ggtggcggcg ctgctggcga
     1561 tgcggtggct cgggtggctc gcgtcgtggc gcgtgccgtc ggcgcgcgcg cggtcgagta
     1621 cgagtccggt cgaggggtcg gcgtggtcgt agaagtattg gaaggagcgg cgctgtaggt
     1681 cttcgagaaa cgcttcgtcc tctgccgtta gacggtatgg ttgcggtggc ggggcggggc
     1741 gttgagcgaa cgccagaggg gagggaacag accagacgag gagaagcagt gtgagcgtgt
     1801 gaagcacgag acggggcgcg cccgcgcgat gagttatgat tttcacttga gcgtatggaa
     1861 tccagagttc accgcggagg cgcggagggc gcggagttcg cgccgcagag attcaaccct
     1921 gcggcatgaa ctctgtgctc cctgcggctc cgcggtgatg cggcgcggca ctaccgctcg
     1981 acgacctcga agtctgcctc cagaccgccg accgaactcg tgccgacgaa gaccttgaag
     2041 gcgcccggct cgaccacgaa gcgcatctcg cggtcgtaaa agccgaggtg cgcggggcta
     2101 agtgtgaact cgactctccg gcgctcgccg ggccggagcg cgacgcgctc gaagcccttc
     2161 aactccctga cgggtcgcgt cacgctcgaa gccacgtcgc gcaggtagag ctggacgacc
     2221 tcgtcgcccg cgcgccgccc cgtgttctcc aactcgacgc tcaacctgag cgacgcgccg
     2281 cgcgtgatgc gccgcgcgct cagttggaga ttcgagagtt gaaactttgt atagctcagc
     2341 ccgtggccga acgggtagag cggcgtccag ggcacgtcca gaaatttgga ggtgtacttg
     2401 ttgttcgcgt ccggcggccg tcccgtgttc ttgtggttgt agtagagcgg cagttgtccc
     2461 acggcgcgcg ggaaggtgac ggggagcttg ccgccggggt tcacgtcgcc gaagagcacg
     2521 tcggcgatgg ccgggccggc ctgtgtgccc gcgaaccacg tctcaaggat ggcgggggag
     2581 ttctcggcgg cccagttgat cgtgagtggc cgcccgttga cgagcacgac ggcgtaaggc
     2641 ttgcccgcgg cgtgaaccgc cttgattaaa tccatctggc ggcccggcag gtcgagcgag
     2701 gtgcgcgaag cggcctcgcc gctcatctcg gccgactcgc cgacgacgac aatgacagcg
     2761 tcggccgcgc gggccgcgcg ggccgcctcg tcgaagttgt cggacgaggc acacaggatg
     2821 tcgcatcccg gcacgtgttt gatgtccgcg cgcgggagtt tttcgcgcag ggccgcgagc
     2881 agcgtcacca catcctcttt gcgcccgtcg cccgtccacg agccgagcat gttggcctga
     2941 tcgtcggcga gcgcgccgat gagggcaagc gagcggatat ttttggcgaa gggcaggatg
     3001 ttgcgctcgt tcttgaggag caccatcgag cgggaggcca cctcgcgcgc ggcggcgagg
     3061 tgcgcggggt tgagcagggc ctcgcgctcg cgcgcctcgt cggcgaaggg cgagtcgaag
     3121 aggccgaggc ggaacttgat acgcaggatt ctacgcaccg cttcgtcgag ccgcgccggc
     3181 gtcagtttgc ccccgcgcag aagctccgcg ccgtgtgtgt tgtagaggcg gctgaccatc
     3241 tccatctcga cgcccgcgtt cagggccaac tgtgccgcct cgcgcccatc cgctgcgacg
     3301 ccgtgcttca acagttcctc gaccgacgtg tagtcgctga cgacgaagcc gtcgaagcgc
     3361 cattcgccgc gcagaacctt cgtgagcgtg aaggggttgg ccgaagtggg cacgccgttg
     3421 aggtcgttga aagcgctcat gaaggtgccg acgccggcct cgaccgccgc cttgaaaggc
     3481 gggaaataga tttcgcggag cgtggtctcc gacacttccg tcgtgttgta gtcgcgcccc
     3541 gcctcggccg cgccgtaagc gacccagtgc ttggcgcagg cgacgacgcg ctcaggcgcg
     3601 gagtagtcag cgccctgaaa gccgcgaaca cgcgcgcgcc ccatcgccgc gccgaggaat
     3661 gggtcttcgc ccgagccttc gacgatgcgg ccccagcgcg cgtcgcgcgc gatgtcgagc
     3721 atcggcgcga acgtccagtg gacgcccgcg gcgcgcgctt ccttcgccgc tacggctgcg
     3781 gcgcgctcga ccgccgccgg gtcccaactg ctggcctcgg ccagcggcac ggggaagatg
     3841 gtgcggtagc cgtggatcac gtcgaagccg aagatgagag ggattttcag acgcgactcg
     3901 tcgagtgcga tgcgttggag ggcgtttgtg ttcttcacgc cgcgcacgtt gagggtcgag
     3961 ccgagacggc ccgcgcggac gagggcgggc tggtcgtcct tggcgcggcc gtcggcgtgc
     4021 ccgtcgagct gctgcaactg cccgagcttc tcttcagtcg tcatccgcgc gagcagcgcg
     4081 tcgattcgtc gctccaggtc gcgccgcgca cgttgcgctt gagtgacggg agtcgtcaca
     4141 gtgatgagca cgaggacgag ggctaaactc gtccgctggg catgattcat ttggaatccc
     4201 tccggcgcaa agtgtcgcac gcggcgcgag atggtgagcc gcgtgcgcgg tcttcccgaa
     4261 aaacgaagtt ggagaaaaat gtgcccgtcc ccacgggcgc gttgattata atagaacttt
     4321 ggccgggcgc gagcagatga acacacaacg cggaaccacg atacacgaaa acactcgatg
     4381 tggcttcggc ccgtgtcgcg ccgcaccccg gcgttgcggg ccgaagacgc acacgcgtct
     4441 actcctgaag acgcttctgg cgtcgctcat tctgctcgcg tcgccgtcga acgcgcccgc
     4501 tcaggggcgg cagaggaaga cgcctcccgc gggcgaacgg agtgaggctc tgagtgagac
     4561 ggtcgcgacg ttgctgcgag acgcgttgga gcttttgcgc gcgggcaagc acgccgaggc
     4621 ggagccgttg gtgcgacgcg tcatcgccgt ccagccgtcg agcgcggacg cgcacggctt
     4681 gctcgggctg attctggatc agggcgggcg cacgcatgag gcggagaggg aatatcgcgc
     4741 ggcgctccgg ctcaaccccc gctcggtctc ggcgcgcgcc aacctcggcg tgctgctggc
     4801 gcgcggactc cggcacgccg aggcgttgcg gctcttcgag tcggtgctcg ccgacgcgcc
     4861 gggccaccca caggcgacgc tcaacctcgg ggccgcgctc tttcacctgc ggcgcttcga
     4921 cgaagccgag cgggcactct ccacggcatc cggcctcgtg ccgcaagccg cggagcctca
     4981 ctactacctc gggcagattg ccgcctcacg tgggcgcggt gaagaggcag ccgagcattg
     5041 gctgcgagca ctcgcgctcc gcgaaaattt cccggaggcg aacttcgcac tcgcggaaga
     5101 gttgcgccgc cagcggcgct acgaaggcgc ggcggagttc tacgggcgtg ccctcgctgg
     5161 ggactcgaag aagcctgtcc atcacatccg tctgggcggc gtgctgatgc tgctcgtgcg
     5221 ttaccaggag gcgctcgccg tcttccggcg cgctgtcgaa gcgttcccca tgtcagcgga
     5281 ggctcattac ttcgccggca tcgcggcgcg cgggcttggg gattacgagg cggcggaggc
     5341 ttcgctgcgg aagtcgttgt tgctcagacc cgacagcgtt gacgcgctcg cgcagacggg
     5401 cttcgtcgtg ggcgagcgcg ggcgtaccca tgaggccgag cgcatcctgc gccgggcggt
     5461 cgcgctcgac cgtcggcact tctacgcgca ttacgatctt ggccggctgc tcgtcagaga
     5521 gcgccgctac gaggaggcga tacccgtcct ccgaagcagc acgggcatca gagaccacga
     5581 ccccggcgtg cattaccagt tgttcatcgc ctactcgcga ctgaagcgta cagacgaggc
     5641 ggcgcgcgag ctgtcaatct tcaagcggct ggaagaggag cgcaaggcgc gtcggagcgc
     5701 gggcggggaa gagcaggtcg aggacacttt gccgcgaccc gcgacagcgg aacccgcaaa
     5761 atctgaagac gaaccggact gagacttcgg aaagggctgg aacgacagcc cctgaaaggg
     5821 gcggaagaat ttagcccacg gctcacgccg tgggctaaat tcttccgccg ctgacgcggc
     5881 ttcgctgcca ccgaaacggc ttcactgaag gccggtgaaa ccggccctca ctttatcgcc
     5941 aaaagtctca gaagctgatg cgtgcctgaa gctcgacgac gcggcccgcg aggccgcgct
     6001 ccgagcgacc gaagtcgggg ttttcgatgt tgggggcgaa gaaacggagc ggcgaaaggt
     6061 tcaggttatt gagggcgttg aagaggttgg cgcgcagctc aaggaacgac ccgtcgccca
     6121 tgaacgcggg cagccccgtc cgcttgccaa ccgtcaggtc gaggctgaag tagcgcggcc
     6181 cgcggaagct gttgcgcccg atgcccggcg gcagcacgcc gcccgggtta ttgatgtcga
     6241 agtacgggcc cccgttcgtc tggatgccgg ggaagttgcc gcccggtcgg atgaaggcgt
     6301 cgttgcccgt gtcttcgagc gcgccgccga aataacgcgc cggacgggcc gggcagagcg
     6361 tcgggccgcc gggcgtgcgt gtgcactcgc cagtcttcgg cgtccaggga aagcccgtgt
     6421 gccacgtcat gatgccgctc agttgaaacc ccccgagcac tttgccgagc gtgtcacggc
     6481 gaccgcggaa gaagggcagc tcccacacgc ccgaggtgac gaggtaatgc ttcacgtcga
     6541 agtctgaagg gccgcgctcg gtgcgcaagt cttgcgggaa agtctggttg gactccgcgc
     6601 cggggccttc gttcgagagc gtgtcgatgc tcttcgagaa gcgatagtaa acgtcgaagc
     6661 gcgtgccggc ggagaagcga cggacgaggc gcgcgttcag gccgttgtag ttcgagttga
     6721 catcgggctg cgggaagaag acggcgaaga aggccgggtt gttcgggtag aggaagttct
     6781 ggttgacgag gcggatgagc ttgtggcccg agcttccctg atagccaagc tcggcggtga
     6841 atttgcccgg cagttcgagc tgcgtttcga gcgaataggt gtagacgtaa gcgttcggga
     6901 tgtccggctc ggagccgtag acctcgacgg aacggccgcg ggccgagccg gtcgccgggt
     6961 cgatgccgac ggcgagcgcg gggttggtcg ggtaactgaa aggcgagttg ctcgaaccga
     7021 gcgcgtagag aatctgcccg tcggcgaagg gcgtactgaa ggagtcgccg cccgccggcc
     7081 ccgcggtgcc gcagcaaatc tggtagcgcg cgaagaaggg cgggttgccg cgcgagtttg
     7141 agaagagcac gttgggcgtg cggttgtagg agatgccgaa gccgccgcgg atgacggcgc
     7201 ggttctcgcg caggaggccg aggaaagagt ctccgtagtt cgggctccag gcgaagccga
     7261 gccggggcgc gaagttgttg cggtcggctt taaacagttc gtccgtgacc tcaaggcgcg
     7321 cgttctgaag gccgttcgag ccgaagacga ggttgctcaa ctgcccgttc ttctctttga
     7381 gcggcgtgaa cagctcgtaa cgcaggccga tgttgagggt cagattgggg cgcgccttcc
     7441 aatcgtcctg cacgaagaag ccgtaatatt tcgagcggaa gtagcgctgc gcgtccgccg
     7501 ggccgcccgt gcgcgggtcg gcgttgatct ggtagaagag cggcgtgtcg ttggcgaagt
     7561 tgaagaggcc gctgaaggtg aagagcgggc gcgagccgcc gttcaggttg ttgttgtctt
     7621 gctcccagcg cagctcgccg ccgaacttga agttgtggct gccgcgcacc acgttgaggg
     7681 tgtcgcgcag ctcgaaagtg ttctgcgcga agatgccggg gctggtctcg ccctgcggcg
     7741 cgccgaagcg gatgcggtcg aagggaagcc cctcgatctc gatacgcggg atgccgaagt
     7801 tggtgtcaac cgccgactcg acttcgttca aggcgaagcg cgtggcgttg aagcgcagct
     7861 cgttgaccat cgtgttcgag agcgtgcgga tgtaggtgat cgcgcccgac gagttgaagg
     7921 gcgtgctgcg gatgtcgctc gaaggacgac cgcccgagcc ttcgtcgctg ccgaggatgt
     7981 cgttgcgcgt gaggaacgtg ctgatggcga tctggtcgcg ctcggtcggc gtgaaatcga
     8041 ggcgcaggtt gtactggttc gagttgatgt cgcgcggcgt ggcgatgagc gcgcgctgaa
     8101 tgtccgggat gccgtcgaag ccgccgccca cggcgttgcc ggtgctgatg tactggcccg
     8161 tcgcgcccgt cggcgagccg atgtcaagcc cgttgcccac gacgcggcag cggctcgggt
     8221 cgttgttgaa ctgggcacaa ttctgcgaga gcaccgagac gatgcgcggc tggatgccct
     8281 gcgaagtgag gatgcgcgcg acgatgccgt tggggcgcgc ggcgatgatc tgctggcgat
     8341 actcgggcgt ctcgacgaac ttcaccgccg agcctgacga gtggctgcgc aggccctcgt
     8401 aggaaaagaa gaagaaagac ttgtcgcgac cgccgatggt cgaggggccg ccctcgccga
     8461 agcgcggcag cggcaacggg ccgccgaagg agccgccgaa ctgcttgaag cggctgctga
     8521 cacgctcggg gccggcgcgg ttgaagtcgg ggccgtactt gttgaaggcg ttccagcggg
     8581 ggtcgttggc cttgaggaag agactgccgt ggaaggtgtt ggttccgttc tgcgaaacga
     8641 cctgaacctg cgcgcccgag ttgcgcccgt actcggcgga gtaggccgaa gagacgacgc
     8701 gcacctcctt gaccgcctcc tggttcggtg tcacgaccgc cgcgccgccg aactggaggc
     8761 tgttgacgct gacgccgtcg atctggaagt tgttggccga gatgcgttgg ccgttggccg
     8821 tgatctgcgg ctggttctcg gcctgaaaaa tcgagttgct cgaaccaccc ggccccgtcg
     8881 agttcgggag cttgaccgac tcgccgacgc cgctgcgcgc gccgctgccg aagacgcccg
     8941 gcgtcaggcg caaaagctcg taggggtcgc gcccgaactg cgggaggcgg cgcacctcct
     9001 cggtggtgat ggccctgctg acgttggcgt tttcggtctc cagcccttgc gcggcttcgc
     9061 tcgtcaccgt gacggtctca gccacctcgc cggtggtcag caccacgtcc atgccctgca
     9121 cgctctcggc gctgaccgtg acgttatcga agaaggtctt cttgaagccc gccttctcgg
     9181 cggtcagcgt gtagcgaccg ggcgcgaggt tgctgaagct gtaaaagcct tcgtcgctgc
     9241 tcgtggtctg ctgcgcgcgg ttcgtctcgg tgttggtcag cgtgacgctg gccccggtga
     9301 cgacggcccc cgccgggtcg ctcacggtcc cctggacgcc cgccctgaac tgcgcctgag
     9361 ccgtcgcggc gaaacacagc aacagcagcg cgagcacggg cgcgcggcgg atgactgatg
     9421 gagttttctt catggtctgt ggccttcctg agcgtaaacg gcgtaaactg cgagccgcac
     9481 ctgaaagatg tcgatgatta aaagtgggcg caccagacac tcgcaaccag tgatgcgtcc
     9541 cgcgaactta caagacgttc gcggaatgta ttgacagagc cggtgactct tggggggatc
     9601 gtcagctatg tgtgtgttgc tcgttgagaa acaaataaca ctcgccgcgc tttttttcaa
     9661 tggaaatctt cgccgcgcct ttgctatctt agacccgccc aagcagtcaa ctcagacgat
     9721 ctacagcagt tcgccgggaa tccttcgtgt aagagcgcgg gcgacgggca cggactcgaa
     9781 gggcgtctgg ttcccgcaaa aagtccggcc ggacgaagcg tcgccccagg gcgggccgcc
     9841 tttcccaacc actgccgccg ctcgcgcgcc ggtcaaatgt ttgcatgaaa actattaagg
     9901 agcataactg atgcgagact tgagaaggag tgtgtttttg ctggctgcgg cgtgcgcgtt
     9961 gtcggtcgcg ggcgcttgcg tccagcccac cgagcaggga ggcgccgcag gcggagcgca
    10021 gaaccgcgcg ccgaagaagg cgggcgagaa ggtgaagatc ggcttctcga tggacacgct
    10081 caaggaggag cgttggcagc gcgacaagga gcacgtcgag cagtacgcgc gtgaggccgg
    10141 cgccgatctc gaagtgcagg tcgctaacgg cgacgacgcc gcgcagacca agcagtgtga
    10201 gacgatgctc acgaagggcg tggacgtttt gatcgtcgcc ccgcacaacg gcgtggccgc
    10261 cgccgccatc gtccagatgg ccaagcagcg cggcgtcccc gtcatctcct acgaccgcct
    10321 catccgcaac tccgacgttg acctctacgt ctcgcatcag gtcgagaaga tgggcgagat
    10381 gcaggggcag tacgcgttcg agaaggtgcc gcgcggcaac tacgtcctca tcggcggcgc
    10441 gcagaccgac aacaacgcgc tgctgctgcg aaagggccag ctcaacgtcc tccagccggc
    10501 catcgaccgc ggcgacatca aggtcatctc cgacctctac gcgcgcgact ggctcgccag
    10561 cgaggcgcag cgcattacgg aagacgccct gacacgcgcc ggcgggaagg ttgacgccat
    10621 cgtcgcctcg aacgacggca cggccggcgg cgccatcgcg ggcctgccgc cggcgctcgt
    10681 cggcaaggtg ttagtcacgg gtcaggacgc cgcgctcgac ggcatccagc gcatcgtcga
    10741 gggcaagcag acgatgacca tctacaaacc catcaagccg ctcgccaaga gcgcggtgga
    10801 cgcggccgtg cagctcgcgc ggggcgaagc gctcagcgcc cccgaccgca tcaacaacgg
    10861 caagatcgac gttccttcca tccttcacgc gccgcaggcc gtggacaagg cgaacgtgat
    10921 gcagaccgtc atcgccgacg gctatcacaa gttcgacgac gtttataaaa acgtgccgcc
    10981 cgatcagcgt ccgaaacaga cgacggcggc cgcgggcgtc gccccgacat tcgtcttccc
    11041 gctgctcttc ctcggcgcgg cgctgttcgc gggagtggtc ttgaagcgat gagcgacgcg
    11101 ccctacccat acgtcccgct gctccagatg cgcgacatca cgaagacgtt cccgggcgtg
    11161 cgcgcactgg acggcgtctc cttcgacctg caccccggcg aggttcacgc cctcgtcggg
    11221 gagaacgggg cggggaagtc tacgctgatg aaggtgctcg gcggcgtcta cccgcacccg
    11281 cagtacggag gggagttttc catcgacggt tacgagcgcc gcttcggcgg cgtgcgcgac
    11341 gccgaagcgg cgggcattgc cgtcatcttt caggagatgt cgctcgtcaa agagctgagc
    11401 atcgccgaaa acatcttcct cggccgcgag ccgcgccgct tcggcatcat ccgctgggaa
    11461 gaactctaca gccgcgcgca aaaactcctc gccgacctcc atctggaggc ggacgcgcgc
    11521 acgcccgtcg gcaacctcgg catcggtcag cagcagctcg tcgagatcgc caaggcgctc
    11581 tcgcaagagg cgcgcatcct cgtcctcgac gagccgacgg ccgcgctcac cgacgccgaa
    11641 gtcgaaaccc tctttcgcat catcgcgaag ctcaaggcgc gcggcgtcgg gatggtctac
    11701 atctcgcaca agctcgacga ggtgtttcgc atctcggagc gcatcacggt gctgcgcgac
    11761 gggcgcaccg tgggcaccga ccgcgccgcc gatctc
//
LOCUS       DASVES010000467         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1644164, whole genome shotgun sequence.
ACCESSION   DASVES010000467 DASVES010000000
VERSION     DASVES010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644164"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF240_19325"
     CDS             complement(<1..287)
                     /locus_tag="VF240_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7177418.1"
                     /translation="METSLDKETREGALGQAATGDGRAVTAAPPVVASPARAREGRAG
                     GDETHSEVKADVQGLSFFYGQKQALNDINMTFPAHRVTAIIGPSGCGKSTLL"
     gene            complement(304..1215)
                     /gene="pstA"
                     /locus_tag="VF240_19330"
     CDS             complement(304..1215)
                     /gene="pstA"
                     /locus_tag="VF240_19330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7177419.1"
                     /translation="MERDFARIIAKRKRQDAAFNVVGIICTLVGILTLGALLFDLLSD
                     GLARLDWAFLTSFPSRFAARAGILSAWVGTLLIMLVTALTAVPLGVAAGIYLEEYARK
                     NWLTNLIEINIANLAGVPSIIYGLMALGLLVYQFRLGQSILTGGLTLAFLILPIVIVA
                     TREALRTIPQGIREAAYALGSTKWQVVRHHLLPYSAGGTMTGIIIALSRAIGETAPLI
                     TIGALTFIAFLPPSPVQTQPPFVSAEWVWSPFAVLPIQMFNWVSRPDQDFVVNAAGAG
                     LVLIVVTLTLNAIAIFIRYRVRKRIKW"
     gene            complement(1215..2183)
                     /gene="pstC"
                     /locus_tag="VF240_19335"
     CDS             complement(1215..2183)
                     /gene="pstC"
                     /locus_tag="VF240_19335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7177420.1"
                     /translation="MKEIPALQHPVAAAADEQQAPRGGELAGTRRLVSVKERVIEWML
                     LAAAFSSVAITVGIVGTLLYESASFFAHVPLSSFLTDTLWTPLFAAPRFGIMPLVAGT
                     LVTTAVALAVALPVGTIVAIYLSEFAPSAAREIIKPVLELLSAVPTVVYGYFALQFMS
                     PLLQKIIPGLPTFNMLSAGVVMGIMIIPYVSSLSEDAMRAVPMSLREGAYALGANRIV
                     TALRVVYPAALSGVVASYILAISRAIGETMIVAIAAGMQPNLTFNPTEPAATITAFIV
                     QVSLGDLPHGSIGYQSIFAAGLTLFVMTLIFNILGYVLRKRFREVY"
BASE COUNT          469 a          737 c          859 g          392 t
ORIGIN      
        1 agcagcgtcg atttgccgca gcccgagggg ccgatgatgg ccgtcacgcg gtgggccggg
       61 aacgtcatgt taatgtcgtt gagcgcctgc ttctgcccgt agaagaagct caagccctgc
      121 acgtcggcct tgacctcgga gtgcgtctcg tcgccgccgg cgcgtccctc acgcgcgcgc
      181 gccggactcg cgaccacggg cggcgcggcc gtcacggccc gcccgtcgcc cgtcgcggcc
      241 tgccccagcg cgccctctcg cgtctctttg tccagactcg tctccatcgt gcttcgtgtc
      301 actttaccac ttgatgcgct tgcgtacccg gtagcggatg aagatggcga tggcgttgag
      361 cgtcagcgtc acgacgatga gcacaagccc tgcgccggcc gcgttgacga cgaaatcctg
      421 atcggggcgc gacacccagt tgaacatctg tatcggcagc acggcaaagg gcgaccacac
      481 ccactcggcc gacacgaagg gcggttgggt ctgcaccggc gacggcggca ggaaggcgat
      541 aaaggtgagc gcgccgatgg tgatgagcgg ggccgtctcg ccgatggcgc gcgagagcgc
      601 gatgatgatg cccgtcatcg tcccgcccgc ggagtagggc agcagatgat gtctgaccac
      661 ctgccacttc gtcgagccta aggcgtaggc cgcctcgcgg atgccctgcg ggatggttcg
      721 caaagcctcg cgcgtggcga cgatgacgat gggcagaatc aggaacgcga gcgtgagtcc
      781 ccccgtcagg atgctctggc cgaggcggaa ctggtagacc aaaagaccga gcgccatcag
      841 cccgtagata atcgacggca cgccggcgag gttggcgatg ttgatttcga tgaggttcgt
      901 cagccagttt ttgcgcgcgt actcttcgag gtagatgccg gccgccacgc ccagaggcac
      961 cgcggtgagc gccgtcacca gcatgatgag gagggtgccg acccacgccg agagaatgcc
     1021 ggcgcgggcg gcgaagcgcg aagggaacga ggtcaggaac gcccagtcga gccgggccag
     1081 tccgtccgac aaaaggtcga agagcagcgc gcctaaggtc aggatgccga cgagcgtgca
     1141 gatgatgccg acgacgttga aggcggcgtc ctgacgcttg cgcttggcga tgatgcgggc
     1201 gaagtcgcgc tccatcagta gacctcccgg aagcgcttgc gcaggacata gccgaggatg
     1261 ttgaagatga gcgtcatcac gaagagcgtc agccccgcgg cgaagatgga ctgatagccg
     1321 atggagccgt gcggcaaatc tccgaggctc acctgaacga tgaaggcggt gatggtcgcc
     1381 gccggctccg tcgggttgaa cgtcaggttg ggctgcatcc cggcggcgat ggcgacgatc
     1441 atcgtctcgc cgatggcccg cgagatggcg aggatgtagg aggcgacgac gcccgagagc
     1501 gccgccgggt agacgacccg caacgccgtg acgatgcggt tagcccctaa ggcgtatgcg
     1561 ccctcgcgca gagacatcgg cacggcccgc atcgcgtctt cgctcaaaga gctgacgtaa
     1621 gggatgatca tgatgcccat gacgacgccg gcgctcagca tgttgaaggt gggcagaccc
     1681 ggaatgatct tttgcaaaag cggcgacatg aactggagcg cgaagtagcc gtagacgacc
     1741 gtgggcaccg cgctcaggag ttcgagcacc ggcttgatga tctcgcgcgc ggctgacggc
     1801 gcgaactcgc tcaggtagat ggcgacgatg gtgcccacgg gcaaagcgac ggcgagcgcg
     1861 acggccgtgg tgacgagggt gccggcgaca agtggcatga tgccgaagcg cggggcggca
     1921 aagagcggcg tccagagcgt gtcggtcagg aagctggaga ggggaacgtg cgcgaagaag
     1981 ctggcggact cgtacagaag tgtgccgacg atgccgaccg tgatggccac cgacgagaag
     2041 gccgccgcca gcagcatcca ttcgatgacg cgctccttca cggagacgag gcgtcgcgtc
     2101 cccgcaagct cgccgccgcg cggggcctgt tgctcgtcgg cggccgccgc gaccgggtgc
     2161 tggagggcgg gaatctcttt catagggagc cgttagctat tagctatcag ctgttagcca
     2221 gaagagctgg caaagtatga ctgaagattt gagcagtagc tccggagaca gtttctacct
     2281 tgagctaaca gctaacagct aacggctaac agcttcctca cgactttggt tcgcgcttga
     2341 gaatctcctc gacggggagg ccgatctcgg gagcgccgcc gaagcccgtg cccgtcttgt
     2401 tgtttcgcag acgctcggtc gccgtctggt aggcgccctc gggcagcggg agatatt
//
LOCUS       DASVES010000468         6053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_657265, whole genome shotgun sequence.
ACCESSION   DASVES010000468 DASVES010000000
VERSION     DASVES010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657265"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2841)
                     /gene="uvrA"
                     /locus_tag="VF240_19340"
     CDS             complement(<1..2841)
                     /gene="uvrA"
                     /locus_tag="VF240_19340"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7177421.1"
                     /translation="MAIDRITVRGARQHNLKDVSVEIPRDRLTVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLDQLERPDVDSVDGLSPAISIEQKTVSRSPRSTVGT
                     VTEIYDYLRLLFSSVGQPHCHVCNAPITRQSVEQIVQSVQSLPEGERVMVLAPIVRGR
                     KGEFKKELEKLSKDGFLRARIDGELISLDEEIKLDKRRNHSIEAVVDRLLIKPGVGER
                     LTESVRTALKLTGGAVVVSVVDGEEKLYSERMACVNCGINVPPLEPRSFSFNSAYGAC
                     KRCHGLGTVLQVDPAKIVTDAGERADKVLQSFSSADKQGAAYLKSALAAVINHYGAAP
                     DVAFGELPRQVREAFFHGVEGQITFRQGLYTYQSDWTGAVRWLTERMNNAPSEKVRLA
                     LEELVSPVVCPDCHGRRLQPESLAVRVGGHGIADYAALTIEDSVKAFEALRLGKREEQ
                     IAGLVLREIRNRLGFLNTVGLGYISLDRPSATLSGGEGQRIRLATQIGSQLRGVLYVL
                     DEPSIGLHPRDNQKLLETLGALRDLGNTVLVVEHDEETIRRADHVIDLGPAAGAHGGQ
                     IVAEGTPEEVACNPESVTGLYICGKLSIEVPRERRTPNGAAVTVRGARHNNLKNIDAT
                     FPLGLLTVVTGVSGSGKSTLVDDILYPALARHVYGSLDEPGAHDSVEGLENVDKVIEI
                     DQSPIGRTPRSNPATYTGLFTPIRELYAMLPESRERGYKPGRFSFNVKGGRCEACEGD
                     GLKRIEMNFLPDVYVLCDVCRGARYNRETLSVKYKGKSIADLLGTNIEDALPLLENIP
                     QIKQKLQTLLDVGLGYVKLGQSATTLSGGEAQRIKLARELSKRATGRTIYILDEPTTG
                     LHFADVHKLLDVLQRLVSLGNTVIVIEHNLDVIKSADHVIDLGPEGGEGGGRVVAAGT
                     PEDVARSRRSYTGQALRPLLNGHVVNGNGHARA"
     gene            3073..4740
                     /locus_tag="VF240_19345"
     CDS             3073..4740
                     /locus_tag="VF240_19345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX7177422.1"
                     /translation="MSERGGQGRLAAQAPMLVMSSRRRWAVTAGVMTGMFLAALEATV
                     VGTAMPTVIASLGGLNHYSWVFSAYLITSTVTVPVWGKLSDLYGRRLLYQVGIAIFLV
                     GSVLSGVSTSMTQLIIFRAVQGLGAGALVPLGMTIIGDIFTVAERARMQALFSGVWGL
                     SSVIGPVVGGFITDQLSWRWVFYINLPVGLAAALIIGLALKEPKRTVRPAIDYAGAGL
                     LMAAITLLMLALVEGGASFRTMLVPRNLALFAGAATLGAVFAWVERRAADPIVPFYLF
                     RNRVVAVAVAAGFMAGVAMFGVVSFVPLFAQGALGATATEAGSLLTPLMLSWVGMSVV
                     GGRLLLRVGYRPTTIAGFIILTLGFVMLSTYQRETARVWLHVDLILIGAGLGLTMLTL
                     LIAVQQAVASTQLGIATSLNQFSRSIGGAVGVAVMGAVLSAGLAARLDEVARGDAGVL
                     TPERAAELSANPNALVDPQAQAALPPGALGALRDALAGSIHNVFWVGTAFSVLALLVS
                     FYLPRASERAGSSPPEEACSAEAGERMMMAELTTIDAEHEPVAARGD"
     gene            4865..5626
                     /gene="pgsA"
                     /locus_tag="VF240_19350"
     CDS             4865..5626
                     /gene="pgsA"
                     /locus_tag="VF240_19350"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7177423.1"
                     /translation="MNLPNSLTLSRIFVVPLLVVVLLTPFSEDWFGLQRHILGATIFL
                     AAAFTDFLDGHIARSRRQVSRLGVLLDPIADKLLISAALISLVENRLAPAWAVCIIIG
                     RELAVTGLRSVAAADGLVIAASRMGKFKMLSQVAAVALLIMSSTRGAPPVANFGDVFP
                     AIQFWTVPELGDAVNHIFGAGVVTATDWQVLLYTAGRAMLWVVVLSSCISMYEYFRGF
                     YLAAVGREAARREATEETAAAATASPATTLAHRGR"
     gene            complement(5583..>6053)
                     /locus_tag="VF240_19355"
     CDS             complement(5583..>6053)
                     /locus_tag="VF240_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7177424.1"
                     /translation="VGGVLVMLAFVGIIAAIAVPNLLASRRAANEASAIANLRQLASA
                     EMTYNALEGEGYADLNELARRQLITSNLAGRELSGYKFEVKVTDEDFEATATPVSYGS
                     TGTRSFYVSTEGIIRVADRRGLKARADDPPLIANLREKEVIIDPGGRVSWPGKQ"
BASE COUNT          889 a         1949 c         2122 g         1093 t
ORIGIN      
        1 ggcgcgagcg tggccgttgc cattcacgac gtgaccgttg aggagggggc gcagagcctg
       61 gccggtgtag gagcggcggg agcgggcgac atcttcgggg gtgccggcgg cgacgacgcg
      121 gccgccgccc tcgccgccct cggggccgag gtcgataaca tgatcggccg acttgatcac
      181 gtcgaggttg tgttcgatga cgatgactgt gttgccgagg gagacgaggc gctggagcac
      241 atcgaggagt ttgtgtacgt cggcgaagtg gagtcccgtc gtcggctcgt cgaggatgta
      301 gatggttctg cccgtagcgc gcttggagag ttcgcgggcg agcttgatgc gctgcgcctc
      361 gccgccggag agcgtggtgg ccgactggcc gagcttgacg tagccgaggc ccacgtcgag
      421 gagcgtctgg agcttctgct tgatctgcgg gatgttctca aggagcggaa gcgcgtcttc
      481 gatgttcgtg ccgaggaggt cggcgatgga cttgcccttg tatttgaccg agagcgtctc
      541 gcggttgtag cgcgcgccgc ggcacacgtc gcagagcacg tacacgtcgg gcaggaagtt
      601 catctcgatg cgcttgagtc cgtcgccctc gcacgcctcg cagcgcccgc ccttgacgtt
      661 gaaggagaag cggcccggct tgtagccacg ctcgcgcgac tcggggagca tcgcgtacag
      721 ctcgcggatg ggcgtgaaca gacccgtgta ggtcgccggg ttcgagcgcg gcgtgcgccc
      781 tatgggcgac tggtcgatct cgatgacctt atcgacgttc tccagccctt cgaccgagtc
      841 gtgcgcgccc ggctcgtcga gtgagccgta gacgtgtcgg gcgagcgccg ggtagaggat
      901 gtcatcgacg agcgttgact tgccggaacc ggagacgccg gtgacgacgg tgagcaggcc
      961 gagagggaac gtggcgtcga tgttcttgag gttgttgtgg cgcgctccgc gcacggtgac
     1021 ggccgcgccg ttgggcgtgc ggcgctcgcg cggcacttcg atggagagct tgccgcagat
     1081 gtagaggccg gtgacggact cggggttgca ggccacctct tcgggcgtgc cctcggcgac
     1141 aatctgtccg ccgtgcgcgc cggccgcggg gccgaggtcg atgacgtggt cggcgcggcg
     1201 gatggtctcc tcgtcgtgct cgacgacgag gacggtgttg cctaagtcgc gcagcgcgcc
     1261 gagggtctcc agaagtttct ggttgtcgcg cgggtgcagg ccgatggaag gctcgtcgag
     1321 gacgtagagc acgccgcgca gttgagagcc gatctgcgtg gcgagacgga tgcgctggcc
     1381 ctcgccgccg gagagcgtgg cggaggggcg gtcgagcgag atatagccga ggcccacggt
     1441 gttgaggaag ccgaggcggt tgcgtatctc gcgcaggacg aggccggcga tctgctcctc
     1501 gcgtttgccg agccgcaacg cctcgaacgc cttcactgag tcttcaatcg tcaacgccgc
     1561 gtagtcggcg atgccgtgac cgccgacgcg cacggccagc gactcgggct gcaagcgacg
     1621 gccgtggcag tcggggcaga cgacgggcga gacgagttct tcgagcgcga gtcgaacctt
     1681 ttccgacggc gcgttgttca tgcgctcggt gagccagcgc acggcgcccg tccagtcgct
     1741 ctggtaagtg taaagtccct gacggaaagt tatctgcccc tcgacgccgt ggaagaaggc
     1801 ttcgcgcacc tgtcgcggca gctcgccgaa cgccacgtcg ggcgcggcgc cgtagtggtt
     1861 gatgacggcg gcgagcgcgc tcttcaagta ggccgcgccc tgcttgtcgg ccgacgagaa
     1921 agattgaaga actttgtcgg cgcgctcgcc cgcgtccgtg acgatcttgg ccgggtcaac
     1981 ctgaaggacg gtgccgaggc cgtggcaacg cttgcaggcg ccgtaggctg agttgaagga
     2041 gaaggagcgc ggttcgagcg gcggcacgtt gatgccgcaa ttcacgcagg ccatgcgctc
     2101 cgagtagagc ttctcctcgc cgtccacgac cgagacgacg accgcgccgc ccgttagctt
     2161 cagggccgtg cgtaccgact cggtgaggcg ctcgccgacg ccgggcttga tgagcagtct
     2221 atcgacgacg gcctcgatgc tgtggttgcg tcgcttgtcg agcttgatct cctcgtcgag
     2281 cgagatgagt tcgccgtcga tgcgcgcgcg caggaagccg tccttcgaaa gcttctccaa
     2341 ctccttcttg aactcgccct tgcgcccgcg gacgatgggc gctaagacca tcacgcgctc
     2401 gccttcgggc agactctgaa cgctctggac gatttgctcg acggactggc gcgtgatggg
     2461 cgcgttgcag acgtggcaat ggggctgccc gacggaggag aacaggagac gcaggtagtc
     2521 gtagatttcc gtgacggtgc cgaccgtcga gcgcgggctg cgagagacgg tcttctgctc
     2581 gatggagatg gcgggcgaca gaccgtcaac ggaatccacg tcaggccgtt cgagttggtc
     2641 gaggaactgc cgggcgtagg ctgagagcga ctcgacgtag cggcgctggc cctcggcgta
     2701 aatcgtgtcg aaggccagag aagacttgcc cgagccggag agtccggtga cgaccgtcaa
     2761 acggtcgcgc ggaatctcga cgctcacgtc cttcaggttg tgctggcgcg ccccgcgcac
     2821 cgtgatgcgg tcaattgcca ttgcctaaaa cattccggga cccgcataga gaagaattcc
     2881 ggcgaccggc gaaacctaca ttataaacga cgcgcgacag ggccggcaac cgagccagcc
     2941 ccgagacact gaaagatttt gaagccggcc accaacgccc cgccaacgct tgccggcacg
     3001 aggcttctca cgaatcacag aacgcgccgt gccacgctgg cggcgtccga cgaactcgga
     3061 tatcttcctc aaatgagtga acgaggggga caggggcgct tagcagcgca ggcgccgatg
     3121 ctcgtcatgt cgtcgcgccg gcgttgggcg gtgacggcgg gcgtgatgac ggggatgttc
     3181 ctcgccgcgc tcgaagcgac cgtggtcggg acggcgatgc cgaccgtcat cgcatcgctc
     3241 ggcgggttga atcactacag ttgggtcttc tcggcctacc tcatcacctc cacggtgacg
     3301 gtgcccgtct ggggcaagct ctccgacctc tatggccgac ggctcctcta tcaagtcggc
     3361 atcgccatct tcctcgtcgg ctccgtgctc tcgggcgtct ccacttcgat gacgcagctc
     3421 atcatcttcc gcgccgtaca gggactgggc gcgggcgcgc tcgtgccgct cggcatgacg
     3481 atcatcggcg acatcttcac cgtggccgag cgcgcgcgga tgcaggcgct ctttagcggc
     3541 gtgtggggat tgtcgagcgt catcggcccc gtggtgggcg gcttcatcac cgaccagttg
     3601 tcgtggcgct gggtcttcta catcaatctt cccgtggggc tggcggcggc gctcatcatc
     3661 ggcctcgcgc tcaaggagcc gaagcgcacc gtgcgccccg cgattgacta cgcgggcgcg
     3721 gggcttttga tggccgccat cacgctgctg atgctggcgc tcgtcgaggg cggcgcgagc
     3781 ttcaggacga tgctcgtgcc gcgcaacctc gcgctcttcg cgggggcggc gacgctcggg
     3841 gccgtgttcg cgtgggtcga gcgccgcgcc gccgacccca tcgtgccttt ttatctattc
     3901 cgaaaccgcg tggtcgccgt cgccgtggcg gcgggcttca tggcgggcgt ggcgatgttc
     3961 ggcgtcgttt ccttcgtccc gctcttcgcg cagggcgcgc tgggcgcgac ggcgaccgag
     4021 gccgggtcgc ttctgactcc gctcatgttg agctgggtcg ggatgtctgt cgtcggcggg
     4081 cggctgctgc tgagggtcgg ctatcgcccg acgactatcg cagggttcat cattctcacg
     4141 ctcggcttcg tcatgctctc gacctaccag cgcgagacgg cgcgcgtctg gctccacgtc
     4201 gatttaatcc tcatcggcgc gggcctcggg ctgacgatgc tgacgctgct catcgcggtg
     4261 cagcaggcgg tcgcgagcac gcagctcggc atcgccacct cgctcaacca gttctcgcgc
     4321 tccatcggcg gcgcggtcgg cgtggccgtc atgggcgcgg tgctttcggc cgggctggcg
     4381 gcgcgtctgg acgaagtggc gcgcggcgac gcgggcgtgc tgacgccgga gcgggcggcg
     4441 gagctatcgg caaaccccaa cgccctcgtc gatccgcagg cgcaggcggc gctgccgccg
     4501 ggggcgctcg gcgcgttgcg cgacgcgctg gccgggtcga ttcataacgt cttctgggtc
     4561 gggacggcgt tctccgtgct ggcgctgctc gtgtccttct acctgccgcg cgcgtctgag
     4621 cgcgccggct cgtcgccgcc cgaagaggcg tgcagcgcgg aggccggcga gcgcatgatg
     4681 atggccgagt tgaccaccat cgacgccgag cacgagccgg tcgcggcgcg cggcgattag
     4741 ggcgcgaact cactttcttg acacccttcg cgggcggcgc ctaaactgcg accggcctcg
     4801 aaggccttct catcccatag aaaagcaaag cccccgcgag cggcgggggc aacagagggg
     4861 gcgcgtgaat cttccgaact cgctgacgct gtcgcgcatc ttcgtcgtcc cgctgctcgt
     4921 cgtcgtcctc ctcacgccgt tctccgaaga ctggttcgga ctccagcgtc acatcctcgg
     4981 cgccacgatc ttcctcgccg ccgccttcac cgacttcctc gacgggcaca tcgcgcgcag
     5041 ccgccgacag gtctcgcgcc tcggggtgct cttagacccc atcgccgaca agctgctcat
     5101 ctcggccgcg ctcatctcgc tcgtcgagaa ccggctggcg ccggcctggg ccgtctgcat
     5161 catcatcggg cgcgagctgg ccgtgacggg cctgcgctcg gtggccgccg ccgacgggtt
     5221 ggtgatcgcg gcctcgcgca tggggaagtt caagatgctc tcgcaggtgg cggccgtggc
     5281 gctgctcatc atgtcgagca cgcggggcgc tcctccggtc gccaacttcg gcgacgtctt
     5341 cccggccatc cagttctgga cggtgccgga gctgggcgac gccgtcaacc acatcttcgg
     5401 cgccggcgtc gtcaccgcga ccgactggca ggtgctcctc tacaccgcgg ggcgcgcgat
     5461 gctctgggtc gtcgtgctct cttcctgcat ctcgatgtac gaatacttcc gcggcttcta
     5521 tctcgcggcc gtcgggcgcg aggcggcccg gcgcgaggcg acggaagaga cggctgccgc
     5581 ggctactgct tccccggcca cgacactcgc ccaccggggt cgatgattac ttccttctcg
     5641 cgcaggtttg cgattagcgg cgggtcgtcg gcgcgggcct tgagtccgcg ccggtcggcg
     5701 acgcggatga tgccctcagt cgagacgtag aaggagcgcg tccccgtcga gccgtagctg
     5761 accggcgtcg ccgttgcctc gaagtcctcg tccgtcacct tcacttcaaa cttgtagccg
     5821 ctcagttcgc gcccggcgag attacttgtt atcaactgcc tgcgggccaa ctcgttcaag
     5881 tccgcgtagc cttcgccttc cagcgcgttg taagtcatct cggccgaggc caactgtcgc
     5941 aggttcgcga tggccgaagc ctcgttggcg gcgcggcgcg acgcgagcag gttggggacg
     6001 gcgatggctg cgatgatacc cacgaacgcc aacatgacga gcacgccgcc cac
//
LOCUS       DASVES010000469        10895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1465503, whole genome shotgun sequence.
ACCESSION   DASVES010000469 DASVES010000000
VERSION     DASVES010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465503"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1178
                     /locus_tag="VF240_19360"
     CDS             192..1178
                     /locus_tag="VF240_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177425.1"
                     /translation="MKLKHRTGTIVRRRAARALPTLALAAAFLFVGHAAPATAAQAKK
                     PISKDGLLKAIRINGLSTAELVKQIEQRGVSFVVTPAVEQEMTASGARPEVVAAARRN
                     YRAGATPAVAAGGGRGGRTNVPPTAKGRARPAPPRGPDYDDLTDQATLAYDTRDPARA
                     VQLLQQAIQLDPSQPRAYQLLGFTQLYLQDDIASAERNMREAITRGGSAGFRVFHDHA
                     EGSFNQTCAGTLFVTQSNVTFKADNGRDTFEALDSQIKEIKVNKLVGRGPLGGLFGGK
                     GDIGKLFGGKGDLGAFHIKVSKNYNFAPLTQKKKESELIIKLVREFGAIKGD"
     gene            1226..2389
                     /locus_tag="VF240_19365"
     CDS             1226..2389
                     /locus_tag="VF240_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177426.1"
                     /translation="MSAHVNTRTHVTRSSRAVRALLLMLLAVACAAPLLAGAATSQAK
                     KPISKKGLINAVKINGLTTREFIREIESRGVDFEMTGAAESEIRGAGARPEIVDAARS
                     NYRGAAARPAVTAARNTSARPNTSTGTSPAASVPSGPPLSKDAIVELLRNGVPAERVE
                     RNVEARGVTFSMTPDIAREIKAAGGTNALVGAITEKAVSGPSSAGNNNEPDDDAGEEA
                     SGPSYEGLIDQAQASFTRQDFNSAGAQLRQAIELDPSRPIGYQLLGSWLLLGGDIASA
                     EKAMRLALERGGSANFRVAHDHDGAFNQYCEGSFFVSKAGVSFVANDGRDTFEAEDAN
                     IKEFKTNRMVGAAYGAFHVKPVQKINNRDNFNFAPLSRNKGESELMIRLFQAF"
     gene            2655..3188
                     /locus_tag="VF240_19370"
     CDS             2655..3188
                     /locus_tag="VF240_19370"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7177427.1"
                     /translation="MSEKIRQKAHVEFTLNGETVEVAFAPHKTLLEVLREDLQLTGTK
                     HGCELGECGTCAVLVDGRSVLSCLMLGLEAEGRRVETVEGMATEEGLHPLQDTFADTG
                     AAQCGYCTPGFLLVAKELLAQQPNPTRDEIKEALSGNLCRCTGYIKIYEAVELAAARM
                     RGERDAALPHESIYGCK"
     gene            3175..5664
                     /locus_tag="VF240_19375"
     CDS             3175..5664
                     /locus_tag="VF240_19375"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7177428.1"
                     /translation="MAASKEQGQSSGDAGKLKVNGAASADGRSLKVVGRPFRKVDARS
                     KCVGETRFADDISLPRMLYCKILRSPEPHALIKSIDTTKAAALPGVLAVITGRDLPIP
                     YGILPVSQDEHPLCVERVRFVGDPVAAVAAVDEDTAFDAMNLIEVEYERLQSITSIEE
                     AVLVEEPRIHDYGDHGNVHKKVHLEFGDLDVGFAEADLVREDIFFYEGNTHLPMEQHA
                     AVAHFDSDQKLTIWSSTQTPHYVHRALAKTLEMTAAHIRVIATPNGGGFGGKSDPFNH
                     EIVVAKLSMITRRPVKVTLTREEVFYCHRGRHPVLMKSRIGVKKDGSITALDFHSFLD
                     GGAYGSYGVASTFYTGALQTVTYDVPRYRFRGLRAFTNKPPCGPKRGHGTPQPRFGLE
                     VQLDKVAEELNLDPAEMRLKHLVKPYSVTANYLRVGSMGLGKCIEKVVEGSDWKNKFR
                     KLPYGRGVGVACSSYICGAGLPIYWNNMPHSGVQLRLDRQGGVCVQCGSTDIGQGSDS
                     ILAYIVAEVLGIDPFDIRVVTADTDLTPVDLGSYSSRVTLMTGNAALQAAERARELLT
                     MAVSEKLGVPVENLSFADRRVFDVENPETGVTFAEAVVLAESKYGTIGTVGSYVPPRS
                     PGKFKGAGVGPSPAYSYSAAVAEVDVDAETGIITVERVWIGHDIGRSINPMLVMGQVE
                     GSVYMGLGEALMEEMIYRANRNVVHKFPSLLEYKSPTTMEMCDVVTYLIEDPDPNGPY
                     GAKEVGQGPLLPVMPAVANAVYDAVGVRVDEVPITPEKVLSALRAKAKGNEPRYGPAS
                     IPEVEWPDPLKVLTPAEGGDGKEMPRVAVHS"
     gene            5683..6021
                     /locus_tag="VF240_19380"
     CDS             5683..6021
                     /locus_tag="VF240_19380"
                     /inference="COORDINATES: protein motif:HMM:NF045464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177429.1"
                     /translation="MKLPNADRATVPVEKLRDYSLNAAHPEGKHKARVFAALLGFTAA
                     DSEKLRHMILAAILTHEAAQGATDEHGTRYRVDFEAEGLRSPVMIRTGWHIDAGETIP
                     RLVSCYVKRK"
     gene            6026..6241
                     /locus_tag="VF240_19385"
     CDS             6026..6241
                     /locus_tag="VF240_19385"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEX7177430.1"
                     /translation="MDEIKVLDVVALTEHVPEHGLRRGEVGTVVDQWKDGVFEVEFSD
                     DTGEAYAFAAVPAGKLMKLYFRKDEAA"
     gene            6323..6937
                     /locus_tag="VF240_19390"
     CDS             6323..6937
                     /locus_tag="VF240_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177431.1"
                     /translation="MADALPQLQMRRKLGSERFRHNDKDLDLTLLDFWRWYASDLVDN
                     TKRGILAEFIVANALGCLNEVCNLGDAYDLELTTGEKIEIKSAAYVQSWYQKKHSPIG
                     FNIRPTRGWKASTGQMEDETKRQADIYIFCLLNHKDKESIDPLNLDQWEFYILSASVL
                     NQACPTQGRIGLTSLERLNPLKASYAEIRPCVEKVAQSLKKTVG"
     gene            6957..7961
                     /locus_tag="VF240_19395"
     CDS             6957..7961
                     /locus_tag="VF240_19395"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7177432.1"
                     /translation="MMRLPKFSYLAPRTVGDAVRMMGDAGPAGQFVAGGTDLYPNMKR
                     RQQTPRVVVSVGRISELQKIEGDGKSGVRIGAGVSLTEIVEHPLINRDYPAVAHAART
                     ISTPTLRNMGTVGGNLLLDTRCNYYDQNYEWRRAINFCLKKDGDICWVAPGSSKCWAV
                     QSADLVPLMVAIGAQVRLVSTVGDRLVSARGLYNDDGIDYLHKRPEELLTEIHLPPVN
                     GHRAIYKKLRRRGAFDFPVLGVAVSLDVARDGTVTDAKVILGGIAPAPMEVEAAGQAL
                     VGGPLDAERIERAAEAAYVRARPLDNTDHLMNYRKQMARPFVRRALEELRQGMRVMQG
                     "
     gene            7972..8670
                     /locus_tag="VF240_19400"
     CDS             7972..8670
                     /locus_tag="VF240_19400"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7177433.1"
                     /translation="MRDEEEPLNGLSDSAPHPSSLIPHPSLSAILLAAGRSRRMGAFK
                     PLLPFGEVTVVERCVSVLREGGANDVVVVVGHRAEEVRAGLAHLPFVRFAFNEDAESE
                     MGVSVARGVESLSEQTEAVLVALVDQPAVPAAVVREIVGAREESKARLVVPTWEGRGG
                     HPVLIDLRFRDELLSLDPAVGLRALFDAHRAEVLRLGVGSPYVVRDMDTWEEYCALHA
                     EVLGRPPDARQVGE"
     gene            8789..9358
                     /locus_tag="VF240_19405"
     CDS             8789..9358
                     /locus_tag="VF240_19405"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7177434.1"
                     /translation="MLNIIPRLKKLTVALIFALALAAGAAPTVSVATTRAGEQLNFSL
                     RTTEGGAISSEMLRGDVVVLAFGASWLPLSREQVKGVQELADRYADRDLRVYWVSTDS
                     DSPKSKNYASDEQLRDFARKNKLKIAVLRDPDGALFKRVGVRGNQLPAVVILDREGSV
                     HGEPIAGIDPRGGLAGQLSGPLNTLLGTQ"
     gene            complement(9550..10191)
                     /locus_tag="VF240_19410"
     CDS             complement(9550..10191)
                     /locus_tag="VF240_19410"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX7177435.1"
                     /translation="MDNEQTPQATGRVGGVGPVGLEPLVLASASPRRAEILRAVGWPF
                     EASPADVDESRREGEEAVAYVRRLARAKAEAVARTRLFGLVLGADTVVVLDGEVLGKP
                     RDASDARRMLRALGGRWHEVLTGVALVRAESGRCVVGHEQTRVRFGELSEAEIDEYVR
                     TGEPADKAGAYAIQGRAALFIEGIEGDFWNVVGLPVRLVYRLVRESEGDAPAA"
     gene            10272..10760
                     /locus_tag="VF240_19415"
     CDS             10272..10760
                     /locus_tag="VF240_19415"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX7177436.1"
                     /translation="MDDLFRAMPKLLRAAGESEEVFEAAACAAWRRVAGEGVRGLAVP
                     FRLYRKTLVVAVTDATWQKQLEAISPQLIFRLNSLLGQAIVTYIEFRIDPQTVRRERA
                     RLRAQEGPAAAATTEAHEEHALQSAGVDLQRAAAAISDEALRRRFLVAAGLYIDAQEN
                     RK"
BASE COUNT         2068 a         3620 c         3421 g         1786 t
ORIGIN      
        1 cgcccgcgac cgccgaggcg agacagggcg gcaacgcgcc cgccgcaagg acacccgcgt
       61 cgaagcccaa gagcggggag tcggccgccg ccgccgaagc cgcccgccgg aaaaggaagg
      121 ccgcgctcaa tgcgctcgac cagtagtgcg tggcgtgggg cgtaggcgcg gccgcgaagt
      181 ttgcaagcgt catgaagctg aaacatcgga cgggaacaat cgtcagacgc cgcgccgcgc
      241 gcgccctgcc gacgctcgcg ctggcggccg ccttcctctt tgtcggccac gccgcgccgg
      301 ccacggccgc gcaggcgaag aagccgatta gcaaggacgg gctcctcaag gccatcagga
      361 tcaacggcct ctcgaccgcc gaactcgtca agcagatcga acagcgcggc gtctccttcg
      421 tggtgacgcc cgccgtggag caggagatga cggcgtccgg cgcgcgcccc gaggtcgtcg
      481 ccgccgcgcg ccgcaactac cgcgccggcg cgaccccggc agttgccgcg ggcggagggc
      541 gcggtgggag gacgaacgtg ccgccgacgg ccaaggggcg tgcgcgcccg gcgcccccgc
      601 gcgggcctga ctacgacgac ctgaccgacc aggccacgct cgcctacgac acgcgcgacc
      661 ccgcccgcgc cgtgcagctc ttgcagcagg ccatccagct cgacccctcg cagccgcgcg
      721 cctaccaatt gctcggcttc acacagctct acctacagga cgacatcgcg tcggccgagc
      781 gcaacatgcg cgaggccatc acgcgcggcg ggtcggccgg cttccgcgtc ttccacgacc
      841 acgccgaggg ctctttcaac cagacctgcg cgggcacgct cttcgtcacg cagtcgaacg
      901 tcaccttcaa ggccgacaac ggccgcgaca ccttcgaggc gctcgactcg cagatcaaag
      961 agatcaaggt caacaagctc gtcggccgcg gccccctcgg cggactgttc ggcggcaagg
     1021 gcgacattgg taagctgttc ggaggcaaag gcgacctcgg cgccttccac atcaaggtct
     1081 cgaagaatta caacttcgcc ccactgactc agaagaagaa ggagtcggag ctgatcatca
     1141 agctcgtcag ggagttcggc gcgatcaagg gcgactgaaa gattttttgg ccgcgcgtct
     1201 acggcgacgc ggcgcggaga cattcatgtc agcacacgtc aacacacgaa cacacgtcac
     1261 acggagcagc cgcgccgtcc gcgcgcttct cttgatgctg ctcgccgtcg cctgcgcggc
     1321 gccccttctc gccggcgcgg ccacgtcgca ggcgaagaag ccgatcagca agaagggcct
     1381 catcaacgcc gttaagatca atggtctgac gacgcgggag tttatccgcg agatcgagtc
     1441 gcgcggcgtg gacttcgaga tgaccggggc ggccgagtcg gagattcgcg gcgcgggcgc
     1501 gcgccccgag atcgttgacg ccgcccgctc caactaccgt ggggcggcgg cgcggcccgc
     1561 cgtcaccgcc gcccgcaaca cgtccgcccg gccgaacacc tcgaccggca cgtcgcccgc
     1621 ggcgagcgtc ccttcggggc cgccgctctc gaaggacgct atcgtcgagt tgctccgcaa
     1681 cggcgtcccg gccgagcgcg tcgaacgcaa cgtcgaggcg cgcggggtca ccttctcgat
     1741 gacgcccgac atcgcgcgcg agatcaaggc cgcgggcgga accaacgcgc tcgtcggcgc
     1801 gatcacggaa aaggccgtct ccggcccttc gtcggccggc aataacaacg aaccggacga
     1861 cgacgcgggc gaggaagcga gcggcccgag ctacgaggga ctcatcgatc aggcgcaggc
     1921 gtctttcacg cggcaggact tcaacagcgc cggcgctcag cttcgacagg ccatcgagct
     1981 tgacccgtcg cggccgatag gctaccaact gctcggctcg tggctcctgc tcgggggcga
     2041 catcgcctcc gccgagaagg ccatgcgcct ggccctcgaa cgcggcggct cggccaactt
     2101 ccgcgtcgcg cacgaccacg acggcgcctt caaccagtac tgcgagggtt cgttcttcgt
     2161 ctcgaaggcg ggcgtgagct tcgtcgccaa cgacggccgc gacaccttcg aggccgagga
     2221 cgcgaacatc aaggagttca agacgaaccg gatggtgggc gccgcctacg gggccttcca
     2281 cgtcaagccc gtgcagaaaa tcaacaaccg cgacaacttc aatttcgccc ccttaagcag
     2341 aaacaagggc gagtcggagc taatgatccg tctgttccag gcgttctgac cgcgccgagc
     2401 gcgaaggcaa aagtaaaaag gcaaaaggca aaagtgaaga ggctaccggc cttcccactt
     2461 ttgccttttg cctttttact tttgcctttc ccctttcgcc tgccccacgc aaaactgtta
     2521 tcatggccct tctagcgaac ccgccccgac gcttcgtctt gccgggggaa tcaacaatca
     2581 cttcgggatt tagcggcggc gcggccggtc ggcggccgcc gccaaaaacg tcccggccgg
     2641 acggacggag actgatgtcg gaaaaaatca gacagaaagc ccatgtcgag ttcaccttga
     2701 acggcgagac ggtcgaggtg gcgtttgcgc cgcacaagac cctgctcgaa gtcctgcgtg
     2761 aagacctcca gctcacgggc acgaagcacg gctgcgaact cggcgagtgc ggcacctgcg
     2821 ccgtcttagt tgacggccgc tccgtgctct cctgtctcat gctcggtctc gaagccgagg
     2881 gccggcgcgt cgagaccgtc gaggggatgg cgaccgaaga gggcctgcac cccttgcagg
     2941 acaccttcgc cgacacgggc gccgcgcagt gcggctactg cacgcccggc ttcctcctcg
     3001 tcgccaagga actgctcgcg cagcagccta accccacgcg cgacgagatc aaagaggccc
     3061 tgagcggcaa cctctgccgc tgcaccggct acatcaaaat ctacgaggcg gtcgagctgg
     3121 ccgcggcgcg catgcgcggc gaacgggacg ccgcgcttcc ccatgagagc atctatggct
     3181 gcaagtaaag agcagggaca gagcagcggc gacgccggca aactcaaggt gaacggcgcg
     3241 gcctcagccg acggccgaag cttgaaggtc gtcggccgac cctttcgcaa ggtggacgcg
     3301 cgctcgaagt gcgtcggcga gacgcgcttc gctgacgaca tcagcttgcc gcgcatgctc
     3361 tactgcaaaa tcctgcgctc gcccgagccg cacgcgctga ttaaatcaat cgacaccacg
     3421 aaggcggcgg cgctccccgg cgtcctcgcc gtcatcaccg gccgtgattt gccgattcct
     3481 tacggcatcc tccccgtcag tcaggacgag cacccgctgt gcgtcgagcg cgtgcgcttc
     3541 gtcggcgacc ccgtagcggc cgtcgcggcc gtggacgaag acacggcgtt cgacgcgatg
     3601 aatctcatcg aggtcgagta cgaacgactg caatccatca ccagcatcga agaggccgtg
     3661 ttggtcgagg agccgcgcat ccacgactac ggcgaccacg gcaacgtcca caagaaggtt
     3721 catctggagt tcggcgactt agacgttggc ttcgccgaag ccgacctcgt ccgcgaagac
     3781 atcttcttct acgagggcaa cacgcacctg ccgatggagc agcacgcggc cgtcgcccac
     3841 ttcgactccg accagaagct caccatctgg agctccacgc agacgccgca ctacgtccat
     3901 cgcgcgctcg ccaagactct ggagatgacg gccgcgcaca tccgcgtcat cgccacgccc
     3961 aacggcggcg gcttcggcgg caagtccgac cccttcaacc acgagatcgt cgtcgccaag
     4021 ctctcgatga ttacgcgccg gcccgtcaag gtgacgctca cgcgcgagga ggtcttctac
     4081 tgccaccgcg gccgtcaccc cgttttgatg aagagccgca tcggcgttaa aaaggatggc
     4141 tcgatcaccg cgctcgactt ccactcgttc ctcgacggcg gggcctacgg ctcctacggc
     4201 gtggcctcga ccttctacac cggcgcgctc cagaccgtca cctacgacgt gccgcgctac
     4261 cgcttccgcg gcctgcgcgc ctttacgaac aagccgccct gcggccccaa gcgcggccac
     4321 ggcacaccgc aaccgcgctt cggcctcgaa gtccagctcg acaaggtcgc cgaggagctt
     4381 aacctcgacc cggccgagat gcgtctcaag catctggtca agccgtactc cgtgacggcc
     4441 aactacctgc gcgtcggctc gatgggtctg ggcaagtgca tcgagaaagt cgtcgaaggc
     4501 tccgactgga agaacaagtt caggaaactt ccctacggcc gcggcgtcgg cgtcgcttgc
     4561 agctcttaca tctgcggcgc gggcctgccg atctactgga acaacatgcc gcactcgggc
     4621 gtgcagctcc ggctcgaccg gcagggcggc gtctgcgtcc agtgcggctc gaccgacatc
     4681 gggcaagggt ctgactccat cctcgcctac atcgtcgccg aagtcctcgg catcgacccc
     4741 ttcgacatcc gcgtcgtcac cgccgacacc gacctcacgc ccgtagactt ggggagctat
     4801 tcgagccgcg tcaccctgat gaccggcaac gccgccctcc aagccgccga gcgcgcacgc
     4861 gaactcctga cgatggccgt ctccgaaaaa ctcggcgtcc ccgtcgaaaa tctctccttc
     4921 gccgaccgcc gcgtcttcga cgtggagaac cccgagacgg gcgtcacctt cgccgaggcc
     4981 gtcgtgcttg ccgagtcgaa gtacggcacc atcggcacgg tcggctccta cgtgccgccg
     5041 cgctcgcccg gcaagttcaa gggcgcgggc gtcgggccgt cgccggccta cagctactcg
     5101 gcggcggtcg ccgaggtcga tgtcgatgcg gagacgggca tcatcaccgt cgagcgcgtc
     5161 tggatcggtc acgacatcgg gcgctccatc aacccgatgc tcgtcatggg gcaggtcgaa
     5221 ggctcggtct acatggggtt gggcgaagcg ctcatggaag agatgatcta ccgcgccaac
     5281 cgcaacgtcg tccacaaatt cccgagcctg ctcgaataca aaagcccgac cacgatggag
     5341 atgtgcgacg tggtcaccta cctcatcgaa gaccccgacc ccaacggccc ctacggcgca
     5401 aaggaagtcg gtcaggggcc gctcctcccc gtgatgccgg ccgtcgcaaa cgccgtctac
     5461 gacgccgtcg gcgtgcgcgt cgatgaagtg cccatcacgc ccgagaaggt tctctcggcc
     5521 ctgcgcgcca aggccaaagg caacgagccg cgctacggcc ccgcctccat ccccgaggtc
     5581 gagtggcccg acccgctcaa agtcttaacg cccgccgagg gcggcgacgg caaagaaatg
     5641 ccgcgcgtgg ccgtgcactc gtgatggctg gcaggtgaca gagtgaaact acctaatgct
     5701 gaccgcgcaa ccgtacccgt tgaaaagctg cgcgattaca gcttgaacgc ggcgcacccg
     5761 gagggcaaac ataaggcgcg cgtttttgcc gccttgctgg gcttcacggc ggcagacagt
     5821 gagaagttgc gtcatatgat cctcgccgcc atcttgacgc acgaagcagc gcaaggggcg
     5881 acggacgaac acggcacgcg ctacagggtt gactttgaag cggagggatt acgcagcccg
     5941 gtgatgattc gcacgggttg gcacattgat gcgggtgaaa ctatcccgcg ccttgttagt
     6001 tgctacgtga agaggaagta aagcgatgga cgagatcaaa gttctcgatg tggtggccct
     6061 gacggaacac gtacccgaac acggcttgcg gcgtggcgag gtcgggacgg tcgttgacca
     6121 gtggaaagat ggagtatttg aagtcgaatt cagcgatgac accggcgaag cctacgcctt
     6181 cgccgccgtg ccggccggaa agttgatgaa gctctacttc cgcaaagatg aagcggcatg
     6241 aagctgccgg aaattctgat gccggccgag ggaggcgaag gcatgaagcc accgcgcgtg
     6301 gccgtgcatt ggtgaggttt ttatggctga cgcattacct cagctacaaa tgagacggaa
     6361 gcttggaagc gagcgttttc gacataacga taaagacctc gatttaactt tgcttgattt
     6421 ttggcgatgg tatgcctccg accttgtgga caacacaaaa cgtggcatct tggcagagtt
     6481 cattgtggct aatgctctag gatgccttaa cgaggtttgc aatctcggag atgcatacga
     6541 cttagaattg acgacggggg agaaaataga gatcaagtca gccgcttatg tccaatcttg
     6601 gtatcaaaag aagcattcac ctattggctt taatatccga ccaacaagag ggtggaaagc
     6661 gagtacgggg cagatggagg atgaaactaa gagacaagct gatatttata tcttctgcct
     6721 tctcaatcac aaagacaagg aatcaattga ccccttaaat ttggatcagt gggagttcta
     6781 cattctttca gcttccgtat taaatcaggc atgtccaact caaggacgaa ttggactcac
     6841 aagtttggaa agactaaatc cgcttaaagc ttcatacgcg gagattagac cttgtgttga
     6901 aaaggtagct cagtctctta aaaagacagt gggatagttt ttttggtatc aagcatatga
     6961 tgcgacttcc taaattctcc tacctcgccc cgcgcacggt gggggacgcg gtgaggatga
     7021 tgggcgatgc ggggccggcg gggcagttcg tcgcgggcgg gacggacttg tatccgaaca
     7081 tgaagcggcg gcagcagacg ccgcgtgtgg tcgtctcggt cgggcgcatc tcggagcttc
     7141 agaagatcga gggcgacggg aaatccggcg tgcgtatcgg cgcgggcgtg agcctgacgg
     7201 agatcgtcga gcacccgttg attaaccgcg actatccggc cgtggcgcac gcggcgcgga
     7261 caatctcgac gccgacgcta cggaacatgg gcaccgtcgg cgggaacctc ctgctcgaca
     7321 cgcgttgcaa ttactacgac cagaattacg agtggcggcg cgccatcaac ttctgtttga
     7381 agaaggacgg ggacatctgc tgggtcgcgc ccggcagctc gaagtgctgg gccgtgcagt
     7441 cggccgacct cgtgccgttg atggtcgcca tcggcgcgca ggtgcgttta gtctcgaccg
     7501 tcggcgaccg gctcgtctcg gccagggggc tttacaacga cgacggcatc gactacctgc
     7561 acaagcgccc cgaggagctg ctcacggaga tacacctccc cccggtgaac ggccatcgcg
     7621 caatctataa gaagctgcgg cggcgcggcg cgttcgactt ccccgtcctt ggcgtggccg
     7681 tctcgctcga cgtggcgcgg gacggcacgg tgacggacgc aaaggtcatc ctcggcggca
     7741 tcgccccggc gccgatggag gtggaggccg cgggacaggc gctcgtcggc gggccgctcg
     7801 acgcggagcg catcgagcgc gcggccgaag ccgcatacgt ccgcgcccgc ccgctcgaca
     7861 acaccgacca cctgatgaac taccgcaagc agatggcgcg tcccttcgtc cgccgcgcgc
     7921 tcgaagaact gcggcaaggg atgagggtca tgcagggatg agggatgagg gatgagggat
     7981 gaagaagaac cgctcaacgg gttgagtgat tcagcccctc atccctcatc cctcatccct
     8041 catccctcgc tctccgccat ccttctcgcc gccgggcgct cgcgacgcat gggggcgttc
     8101 aagccgctgc tgccgttcgg cgaggtgacg gtggttgaaa gatgtgtgtc ggtgctgcgt
     8161 gaaggcgggg ccaatgacgt cgtcgtcgtc gtcgggcatc gcgcggaaga ggtgagggcg
     8221 ggactggcgc atctgccgtt cgtgcggttc gcgttcaacg aggacgcgga aagcgagatg
     8281 ggcgtgtcgg tggcgcgggg cgtcgagtct ctttccgaac agacggaggc ggtgctggtc
     8341 gcgctcgtgg atcagccggc ggtgccggcg gcggtcgttc gggaaatcgt cggggcgcgc
     8401 gaggagtcga aggcgcggct cgtcgtgccc acgtgggagg ggcggggcgg gcatcccgtc
     8461 ttaatcgacc tccggttccg cgacgaactc ctgagtctcg acccggccgt gggcctgcgc
     8521 gcactcttcg acgcgcaccg cgccgaggtc ttgcgcctcg gcgtcggctc gccgtatgtt
     8581 gtccgcgaca tggacacttg ggaggagtat tgcgcgctgc acgcggaggt gttgggccgc
     8641 ccgcccgacg ctcgacaggt cggggaatga cagttttcgc ggcccacttc gtaccgcttc
     8701 catccaacta ttctttttga cagcacatca aagattcgca cgggccgaat ccccgcacgc
     8761 ggacgcggca ggctcggagg agacgtagat gctgaatatt atcccccgtt tgaagaaatt
     8821 gaccgtggcg ctcatcttcg cgctggccct tgcggcgggc gcggccccga ccgtcagcgt
     8881 ggcgacgacg cgcgcgggcg agcagttgaa cttttctttg cggacgaccg agggcggcgc
     8941 catctcttcg gagatgctgc gcggcgatgt ggtcgtgctc gccttcgggg cctcctggct
     9001 gccgctctcg cgcgagcagg tgaagggcgt gcaggagttg gccgaccgct atgccgaccg
     9061 cgatttgaga gtctactggg tgagcactga ctccgactcg ccgaagtcga agaactacgc
     9121 gtcggacgaa cagctacgcg acttcgcccg caagaacaaa ctgaagatcg ccgtcctgcg
     9181 cgaccccgac ggcgcgctct tcaagcgcgt gggcgtgcgc ggcaaccagc tcccggccgt
     9241 cgtcatcctc gaccgcgagg gcagcgtcca cggcgagcca atcgccggca tcgacccgag
     9301 gggcggcctc gccggccagc tctcggggcc gctcaacaca ttgctcggca cacagtaatt
     9361 ctcatccact cagtttaccg aacgtttatc acagtcatca gttgagtggt tagtaaggca
     9421 gactcaccgc aaaggcgcag aggacgcata gttagcgcag agaagaagca tcaagcctct
     9481 gcgttaacta tgcgtcctct gtgcctttgc ggtgaatcta tttccctctg actcaagtaa
     9541 tcatcgccct cacgctgccg gggcgtcgcc ctcggactcg cggacgagtc tgtagacgag
     9601 cctcacgggc aggccgacga cgttccagaa gtcgccctcg atgccttcga tgaagagcgc
     9661 ggcgcggccc tgtatggcgt aggctccggc cttgtccgcc ggctcgcccg tgcggacata
     9721 ctcgtcgatc tctgcttccg acaactcgcc gaagcgcacg cgcgtctgct cgtgcccgac
     9781 gacgcatcgg cccgactcgg cgcgcaccag cgccacacct gtcaagacct cgtgccaacg
     9841 accgccgaga gcgcgcagca tccggcgcgc gtccgacgcg tcgcgcggct tgccgagcac
     9901 ctcgccgtct aagacgacta ccgtgtccgc gccgagcaca agcccgaaca ggcgcgtgcg
     9961 ggcgacggcc tcggccttcg cgcgcgcgag ccgccggacg taggcgaccg cttcctcgcc
    10021 ctctcgccgc gactcgtcca cgtcggccgg actcgcctcg aagggccagc cgaccgcgcg
    10081 caaaatctcc gcccggcgcg ggcttgccga cgccagcacc agcggctcca aaccgaccgg
    10141 ccccaccccg cccacgcggc ccgtcgcttg aggcgtctgc tcgttatcca ttacactccc
    10201 cattcgattg cggattctat agcagtcagt cgtcagctaa aagctgaaag ctaatcgctg
    10261 aaagctcttt catggacgat ctctttcgag cgatgccgaa actcctgcgg gcggccgggg
    10321 agtcggagga ggtgtttgag gcggcggcct gcgcggcctg gcgccgggtg gccggcgagg
    10381 gcgtgcgggg tctggccgtc cccttccgcc tctaccgcaa gacgctcgtc gtggccgtca
    10441 cggacgcgac ctggcagaag cagctcgagg ccatcagccc gcaactcatc ttccgcctca
    10501 actccctgct cgggcaggcg attgtgacgt acattgagtt tcgcatcgac ccgcagaccg
    10561 tccgccgcga gcgcgcgcgc ctgcgggcgc aggaaggccc cgccgccgcc gcaaccaccg
    10621 aggcccacga ggaacacgcc ctccagagcg ccggcgttga cctccaaagg gctgccgccg
    10681 ccatcagcga cgaagccctg cgccgccgct tcctcgtcgc cgccggtctc tacatcgacg
    10741 cgcaggagaa cagaaagtga gaagcagtga gcagtaagca gtaggcgccc cgcgaagctc
    10801 gccgctcacc atcgaacttg tttttactgc ttactgctca ctgcttactg cttactactt
    10861 gagcttatgc ccatcaccga agacgacgtt aagaa
//
LOCUS       DASVES010000470        34581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1165443, whole genome shotgun sequence.
ACCESSION   DASVES010000470 DASVES010000000
VERSION     DASVES010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 34581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34581
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165443"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1136
                     /locus_tag="VF240_19420"
     CDS             204..1136
                     /locus_tag="VF240_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7177437.1"
                     /translation="MNKLRFAFTALAFMAFTLAVSSSAQAQATRTWVSGVGDDVNPCS
                     RTAPCKTYAGAISKTAKDGEISTLDPGGFGTITTTKSITINGGGAGQGYGSILSALAP
                     QGVLVNITDVNDIRKSVKLNWLDINGASTGTDGIRMIAGNTLHIENTFIDGMAGDGVD
                     INVSVGIAFSMKNVSIRNCVGSGVKMASTGGGSVGFSLNNVQVSKTGTGIDVQNGARG
                     TITDTVIALNTTGISVTEAGNDAEANIVNATITGHVTGISVGAGARARIARCLITQNV
                     TSLTPAAQVDTGNNNTIMGNTNNTAPAGLPFPQN"
     gene            1436..2359
                     /locus_tag="VF240_19425"
     CDS             1436..2359
                     /locus_tag="VF240_19425"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177438.1"
                     /translation="MLTKPGIDALGPNAASAPPDASPTPPALPRYEALPDAPLVTIEP
                     GKSWSALDLRELWDYRELLYFLIWRDVKVRYKQTVLGAAWAVLQPLFMMALFSLLFGR
                     VAGIDTGAVPYPLFAFVGLVPWTFFANAVTTSSNSLVSSTHLISKVYFPRLLVPAGAV
                     AACLFDFALSFLALLGMLVYYRVSPTANLLMLPALVALMAGFALGVGMWLSSLNVKYR
                     DVRFVLPFLIQLWLFASSVILPSSAVPERLRWLLYLNPMSGVVEGFRSALLGLPFDWR
                     AIGVAATATAVVLVYAAYSFRRTERSFADII"
     gene            2368..3636
                     /locus_tag="VF240_19430"
     CDS             2368..3636
                     /locus_tag="VF240_19430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX7177439.1"
                     /translation="MRPIVRVENVGKLYQLGSSGAAYGTLRDTLAEAVRSPFSRWRGD
                     AGAATSEQSFWALRGVSFDVRPGEIVGIIGRNGAGKSTLLKILSRITEPTTGRIELYG
                     RVGSLLEVGTGFHPELSGRENVFLNGAILGMGRDEIEKKFDEIVAFAEIEKFLDTPVK
                     RYSSGMYIRLAFAVAAHLESEIMIVDEVLAVGDAQFQEKCLGKMGEIARGGRTVLLVS
                     HNVGIVSSLCTRAVYLADGQLRSEGPAEKIVGEYLSEALESKVHDLERLRPAGMGERV
                     KFLDISLASTEGSHVEVGRPLEFLLTIRSEDDMRGLSIGASIFNVVGACVGSLITREE
                     FSVRAGEVVCLRLKVSALSLAPGSYYSGFSIGRGGHEVRRADLDAVLGVPSFQVLPIS
                     DNYLFTNWQLGWGSVIFKDTLLTVVGDVEV"
     gene            3845..4687
                     /locus_tag="VF240_19435"
     CDS             3845..4687
                     /locus_tag="VF240_19435"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX7177440.1"
                     /translation="MNQTLSFVLDPVKLDELAEKYREAYAQAAPFPHIVIDDFLPADA
                     LRQVLSEFPGARQIDWNKFNDPAQRKLGSTAESQIGEYTRFLLYQFNSSTFIRFLEKL
                     TGISGLLPDPHFIGGGIHQILRGGFLKIHADFNRHPLLPLDRRLNLLLYLNEDWREEY
                     GGHLELWSRDMSECRARILPVFNRCVIFSTTDYSFHGHPDPLKCPQERTRKSMALYYY
                     SNGRPEEESSPEHGTLFQHRPGESYDLSAVVSPPQPLTAKQIIIKFVPPIVMEAYLSL
                     KRRV"
     gene            4939..5709
                     /locus_tag="VF240_19440"
     CDS             4939..5709
                     /locus_tag="VF240_19440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7177441.1"
                     /translation="MRSIKSFVPYRVQNAMRVLLGISAIEARMSELEERLGRSEDKLD
                     EFEKISFSKDFWERSRVRWRGNEPSTGLTWNKELKGDNFISKVASYNAFGPDSAVLEI
                     GPGYGRLLKAMLTQGVSFKNYLGVDLSPDRVRQLKETFANEKIDFRQGDVENVALDGT
                     FDVVISSLTFKHLYPTFEKSLANLTTYVNLNGMLFFDLVEGKANFFEYGGETYLHFYQ
                     KSEVLEILGRTNLELVAFDQVEHDPEHVRLLVVAKKVK"
     gene            6102..6554
                     /locus_tag="VF240_19445"
     CDS             6102..6554
                     /locus_tag="VF240_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177442.1"
                     /translation="MESIRNLQRKLGGVGYATRVKLATLLLLALTALLTNLIYLRENR
                     GLEPTTAGHDDINIYERRFGVLREALPARGVVGYLSDEQEVNEATKKYYLTQYTLAPL
                     IVVRDADQALVVGDFARAREGAPETVAAQLRLIKDFGDGVALYAREER"
     gene            6554..8056
                     /locus_tag="VF240_19450"
     CDS             6554..8056
                     /locus_tag="VF240_19450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7177443.1"
                     /translation="MTAVLFALSSLVALAFGFLFVSLLWPAGRPFGERLLLKLCLAAG
                     FGYGLTSCLCFVWLLMHGRADRTYLLFEAVVLALLLAGVLFRLSRARGRSTVAEGERA
                     RESARARARERESDAPPERWGWLFTAFFGVSLASTALAVAAAIVREPHGGGWDAWGIY
                     GLRARAIFRGGENWREGFSALTPWSHPDYPLLLPLSDVRSWLYCGGEVAYAQSVLSAI
                     FTLATAGLLYSALAALRGRAQGYLAGVVLMGFTTFIVYGSSAYADVPVMFFFTATLAL
                     FALHFGSGEGSRQFLILAGVAAGLAAWTKNEGLLFVAAVLAAHLAVTAFAGGRKTYAR
                     QLFFVALGLLPVLAVVVYFKTWVAPPSELTAAMGSSSALGKIFDYHRYVHIVREFLLQ
                     LLYYRGRGVNLTYLLAIYLVCAGVSRKHRSGVIHVGLTLCLMLAGYFWVYLTTPYELV
                     FHVRFSMDRLLLQLWPGFLLLYFLAATAPEEAFGQAASGGRLRASSGVAA"
     gene            8075..8866
                     /locus_tag="VF240_19455"
     CDS             8075..8866
                     /locus_tag="VF240_19455"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7177444.1"
                     /translation="MNNEPCLSVVVPAYNEEATLEEVILKVLALPQLLEVVVVDDCSS
                     DGTPEVTRRLADAYPRVRVARHERNAGKTEALKTGFALTRGGIVIVQDADLEYDPTEI
                     PAVIQPIIEGHADVVYGSRFMVRRAARVLYYYHFLANKFLTSFSNVMTNLNMTDVETG
                     YKAFRGEIIRNMVITSSGFGFEIEVTAKIAKLKCAIYEVPISYYGRTYEEGKKIGLSD
                     GFAALWFVVRFNLFCGLDASFRSRPAPRGEQVCAPDPARNSGALR"
     gene            8866..9591
                     /locus_tag="VF240_19460"
     CDS             8866..9591
                     /locus_tag="VF240_19460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7177445.1"
                     /translation="MSEKIVYVGRDLEAMSFAVNYHRWILDAFGPRLGARLVEVGAGS
                     GEFSELLLAREPETISLVEPSADMHRLLEARVHAAGAGANVATYNATFADVAERIRAE
                     QRPDSVIYVNVLEHVQDDEAELRAVQRTLGAGGRIFIFVPALPWLYGEFDRRVGHYRR
                     YTKAELEEKCQRAGFRMLKSVYFDLAGIAPWWIQYRLLRASTLSPGAVKLYDRLVVPV
                     TRAVESLINPPLGKNLLLIAEKT"
     gene            complement(9588..9710)
                     /locus_tag="VF240_19465"
     CDS             complement(9588..9710)
                     /locus_tag="VF240_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177446.1"
                     /translation="MSSKYYRVRPGISNQQALQLQPQPRLAANRAALAPARLAI"
     gene            9752..10684
                     /locus_tag="VF240_19470"
     CDS             9752..10684
                     /locus_tag="VF240_19470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7177447.1"
                     /translation="MPDFNPLDHLICLQHPARLAPSTWIEHVPFAMYLIDILRPRVFV
                     ELGTFYGVSYCAFCQAVEELGAGTRCYAVDTWQGDPQAGFFADEVLNHLREHHDPLYG
                     GFSRLIQSDFDEAAGQFPEGSVDLLHIDGFHTYEAVRHDFETWLPKMSERGVVLFHDI
                     SVRNEGFGVWKFWDEVKAGRPHYEVDFGWGLGLIATGRERPAALSVLFDSPDDELRRI
                     REYFHRLGLGLEAAQELHSLKKATRARDEASRAREERMRRNHPLLTRASNLLQVCAEH
                     GLGAALRAGQEKIRRKGEASGRSAGEDAATAANS"
     gene            10702..11667
                     /locus_tag="VF240_19475"
     CDS             10702..11667
                     /locus_tag="VF240_19475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7177448.1"
                     /translation="MEAPPKVSVITPLYNSSAFIRATLDSLRAQTYEDWESILVDDGS
                     TDDTAEVVRPYLEDARFRLIRQENRGIAGARNTAIQAASGKWVCLLDHDDRWLPRKLE
                     KQIAFAREHSFDITCTDAFIVTENERWVYSRGFPEVAAEVQRAAHDPSVDVFGLLIKL
                     DFLCASSVMIRRTLFDERGLLDPSAVPADDYEMWLRCLPEAKLGFLGEPLIEYMVHAN
                     NYSRDEPRMIEQAIRVLQLHRKRHALSALRRRQFDESLAYQFGLLFKKLSLDRSKAYA
                     LTRSLSLMKDGPGALRLLRFAVWRPLAERARHAARYRLGLAPRKV"
     gene            11687..12883
                     /locus_tag="VF240_19480"
     CDS             11687..12883
                     /locus_tag="VF240_19480"
                     /inference="COORDINATES: protein motif:HMM:NF024868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177449.1"
                     /translation="MRILFVAMANSIHTARWISQLEGQGWDVHLFPAEEVCPHPRLRN
                     LKLHEPLSVRPPGLDPSVEVIGEWPLPKGASLARRVAGKFAPSWPDRAWHLARTIRKL
                     RPDIVHSLEIQHAGYLTLGARAHFAADDFPTWVVSNWGSDIYLFGRLAAHAGKIREMM
                     AACDYYHCECRRDVALAREFGFEGEVMPVFPVTGGFEVERMRALRQPGPTSSKRRVVL
                     KGYQNWAGRALVGLRAIELCADLMRGYRVTVYLAQPDVMLAAELLSQKTGIPIECESG
                     EWTHDDVLRMHGSARTSIGLSISDAISTSLLEAMIMGSFPIQSHTGCGDEWVKSGENG
                     LLVHPESPEEVAAALRRALTDDALVDRAAEINARLTAERLDKTLIAAMTVEMYKKIAS
                     PRTSAR"
     gene            12932..13639
                     /locus_tag="VF240_19485"
     CDS             12932..13639
                     /locus_tag="VF240_19485"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7177450.1"
                     /translation="MSNLTAILLCGGKGERLRPFTDTAPKTLVPLGGRPLLHHLLSYL
                     AAEGVSRFVACVGYKAEAVEEFLRERRDPSWEVECVNSGDASMTDRILDARAHVGGRA
                     LICYGDTLANVDIGELLASHRASGALATLTTYPLHSPFGIVHFDGSGRVSEFAEKPAL
                     PYWINIGFILCEPEAFAYLLPGSDMPTFLSALAAAGRLYAYRHTGKHLTVNTEKERAD
                     AEVEMVEFFTYMDGQQR"
     gene            13636..14589
                     /locus_tag="VF240_19490"
     CDS             13636..14589
                     /locus_tag="VF240_19490"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7177451.1"
                     /translation="MNKKRVLVTGGSGFIGSHLVHRLLDDGAKVAVTVRYGNVMKNER
                     LRGCWDDLEVIEADLRNRGALACIRDFAPEVVFHLAAYNHVGQSFVQVEECFDVNAKG
                     SANLLDTCGGAARFVYMSTSEVYGHQTEVPFVETMDPEPISPYAITKYAGELYCRMKQ
                     RIGGDTSVVILRPFNTFGPYQSAKAVIPELIVNCLKGEPIRTTKGEQTREFNYVSNIV
                     DGLVAAGSYEGKLDGVLNLASGEEVAIHDLVRKIAELTETRSEIEIGALPYRPTEIWR
                     MYADSARARQTLGWEPKVGLDEGLRLTVEWFREYRRGDSDA"
     gene            14610..15503
                     /locus_tag="VF240_19495"
     CDS             14610..15503
                     /locus_tag="VF240_19495"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7177452.1"
                     /translation="MRVLILGGSGMLGHKLWQTLSERFDTRATFRQSPAAYARYGIFD
                     PARSIGHVSAQDFDGVVRALAAVRPDVVVNCVGIVKQDAAAKDPYQSINTNALFPHRL
                     AQACIASGARLIHISTDCVFTGRRGNYKETDAADAEDIYGRTKWLGEVDSERCLTIRT
                     SMIGRELQGAHGLVEWFLSQRGQRVRGFKRAVFSGFTTEALASIIGDVIAGHAGLRGV
                     WHVAAEPVNKFDLLTLVKEAYGLDIEIEPDETFVCDRSLDGARFRQATGFAPPSWPEM
                     IERMRGDATPYEEFRRIHAQG"
     gene            15490..16548
                     /locus_tag="VF240_19500"
     CDS             15490..16548
                     /locus_tag="VF240_19500"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7177453.1"
                     /translation="MLKDKRVVVTGGTGSLGKVLVRRLLSGEVGLPRQIVVFSRDEAK
                     QHSMRLAFQHLRTATDEVIYHNFEQLLQFRIGDVRDPASVNAVLRHADVVFNAAALKQ
                     VPSCEYFPFEAVQTNIVGAENIVRAVREGRLPVETVVGISTDKACKPVNVMGMTKAVQ
                     ERLFVCANLDVPDTRFVCVRYGNVLASRGSVIPLFHEQIRRGGPVTITTADMTRFLLS
                     LEQAVDTVFAALGEAERGETYIPRVPSARVTDVSDALIGDQKIKTVVTGIRPGEKVHE
                     ILVSEEEAFRTVARGDYYVIQPMLPELRQSEEIKNALGREYSSADNVMSPAEVAELLK
                     RHKLMPSDRFVYEEDMLA"
     gene            16545..17642
                     /gene="wecB"
                     /locus_tag="VF240_19505"
     CDS             16545..17642
                     /gene="wecB"
                     /locus_tag="VF240_19505"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7177454.1"
                     /translation="MKVMTILGTRPEIIRLSRVVEKLDRLCQHVLVHTGQNFDAALSD
                     LFFEELGVRRPDHYLGVRGDTFGEQIGRIISESERVMTAERPDRLLILGDTNSALSAL
                     VAKRLGIPVYHMEAGNRCYDDRVPEEVNRRVVDHSSDVLMPYTERSRQNLLREGFAGE
                     RVYVTGNPILEVIEHYNERVRASDVLARLSLEAQKYFLVTMHRAENVDVEERLRSLIG
                     ALEALGREYGLPVVISTHPRTRAVMERFRVDAGAHAELQFHAPFGLFDFVALEQNAFC
                     VLSDSGTVQEECCIFGVANVTIRDVTERPETIESGSNILSGAEPEAVLRCVRAVLDGK
                     SEWAPPPEYLVKNVSGTVARIVLGYRREGRG"
     gene            17784..19004
                     /locus_tag="VF240_19510"
     CDS             17784..19004
                     /locus_tag="VF240_19510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177455.1"
                     /translation="MKIALVSEALPPSASGQSMVLSRLLGGLDPADYCLISNLNYDAA
                     APHNAYTQRLPVNYHHLSAPRQITRGSRFGLIHLRHGLNFPLTVAQWGRRIADIVRRE
                     RCEAVVACTGEVSLLPAGYLGARLAGVPFFAYVFDHYSYREWANPAARFWARRLEARL
                     LRGAAGVIVPNEILRDDLRRRCGVESEVIHNSFDISDYESAPPPPPSPNGEVKIVYTG
                     DVYEAHYDAFQNLLAALRLLGRDDVRLHLYTSRTPEEMAARGISGPVRIHPHRALAEM
                     PRVQQEADILFLALAFDSPYPDLVKTSATMKLGEFLATRRPVIVHAPPDSFISWYFRT
                     HECGVVVDRRDPELLARKITELLADPELQRRLTERAWGRARADFSIAGARAKFLDVLR
                     RGVRARAPEQEPRP"
     gene            19001..20029
                     /locus_tag="VF240_19515"
     CDS             19001..20029
                     /locus_tag="VF240_19515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177456.1"
                     /translation="MTPDEAAAATPPRAAPLVSVVMPTYNYARYVGDALRSLQAQTHA
                     RWECVVVDDGSTDDTEEVVAGFAARDARIRYVRQENRRQAAARNNGLRHCAGDYVQFL
                     DADDLLESRKLERQAADLEAHPEVDIVYGGARGFTSAGQSGEGAALDDSPLPAMPEAS
                     GGGRELAPGLVRRNIMFVNSPLVRRRLIEEVGSFDERLPPAEDWDYWLRCALKGARFQ
                     FVPEEGTLALVRTHPVSSSRNRAVAYASLLRMRGKLAGLTDDAELLALNRELAAADEG
                     YLGVEEAAAGRVLKGVLHLGRAALRVRRARWRAKWLLCALSAPFVSPPTLRKLATESI
                     TRRRARTT"
     gene            20049..20582
                     /gene="rfbC"
                     /locus_tag="VF240_19520"
     CDS             20049..20582
                     /gene="rfbC"
                     /locus_tag="VF240_19520"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX7177457.1"
                     /translation="MIIRDTKLAGAFLVEPELREDERGFFTHVWTRRELAERGVACDF
                     AECNASFSRRRGTLRGMHYQAAPHGQAKLVRCTHGSIYDVGIDLRPDSPTFRQWVGFE
                     LSARNRLMLYLPAEFAHGFQTLEDDTEVLYMTSAPYAPDSGRGVRWDDPAFGVRWPEV
                     GERTLIARDREYPDFAA"
     gene            20603..21469
                     /locus_tag="VF240_19525"
     CDS             20603..21469
                     /locus_tag="VF240_19525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7177458.1"
                     /translation="MDTPRITVVTPSFNQGQFIEETILSVIGQQYPNLEYIIIDGGST
                     DASVEVIKKYEQHLAYWVSEPDRGQAHAINKGFAKATGRILAWLNSDDMYLPGAFAFG
                     VSALKADAAPGLLFGNCLHFVEGDTQAYGSDVRAGHETMNLTLADYIIQPSTFWTRSA
                     WEATGPLDESLNFGFDWEWFIRARKAGVAFVPADKYLSLYRIHGAHKTGTGGERRLQE
                     LATIYGRHAGAKYERLFARCMASRSKIVAGRTLLGRLRLSRFEAAALKLRFPGLFRGF
                     EPEEISDMMTML"
     gene            21600..22481
                     /locus_tag="VF240_19530"
     CDS             21600..22481
                     /locus_tag="VF240_19530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7177459.1"
                     /translation="MRPKISIVTPSFNQAAYLEQTILSVVGQNYEPLEFIIIDGGSTD
                     DSVEVIKKYEQHLAHWVSEPDRGQAHAINKGLAHATGDLVAYINSDDLYLPGAFAAVT
                     RHFDEHPRCEWLCGDTLMFGEGGVDQTSLVSANVPRTAAQVLSWAYKAPQPGMFWRRE
                     LLRDGFAEQWRYCFDHELYVRLLLDGHACEHLPLPVAAYRLHATSKTVAEGDLFDREF
                     DDIADLYEPRLRGAGRRWCAATRHLRHSFAASRRGDRREALAGLARALFVHPESLGGR
                     PFWGCFRRALGPQAGKP"
     gene            22744..24480
                     /locus_tag="VF240_19535"
     CDS             22744..24480
                     /locus_tag="VF240_19535"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177460.1"
                     /translation="MSSTEATSDSLTGTVKGPGERPHEYLFITADQLRARIGEFVYYV
                     ARDGREQRRIIGNVTSRRLVRNLPDSFSADPETPPAVISSLIGLDGDGCELYEITVES
                     IGYFSERLGDFVNPRIPPRPGDPVFLASSETLSGVLSSRRAGERGSTHVGSLLTRGAG
                     EVPIVLSTRDVVSTHLAILASTGAGKSYTAGVIVEELMMPYNRAAVLVVDPHGEYDTL
                     RSVEEDARFSDPADGYRPEVKIFTHDRVKVRFSSLTEADIKYLLPEGTSEKMLHYLSQ
                     AFRKVTARAREGHAGGSYLWGYHDLRDAVTEMKYGDSRSGGEDGMGGSSSNAPTIEAL
                     LWRLDNRFDRPNSLFSDSEHIPLNELFRPGRCTVLQLSDIEQNEQQVVVATLLRRVNK
                     ARVATVREESEARTDTHLPYPVFTLIEEAHRFAPAGQTVVSTNILKQILSEGRKFGVG
                     IGLITQRPGKLDQDVLSQCMTQVIMRIVNPVDQDTVAKSVEGAGRQLIAELPALTKGQ
                     AVISGVGVNTPVMCRVRERLTRHGGETFDAPDEWARYHSKAEQQRREQDDAILVSPAR
                     PKKADKAGPFQI"
     gene            24675..25163
                     /locus_tag="VF240_19540"
     CDS             24675..25163
                     /locus_tag="VF240_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177461.1"
                     /translation="MISRPRPLAPLLLLLLLPLLLLLTLSAIGPAPARAQMGDAGKNE
                     DPVFKEFKGVSIGTPMDEARKKLGSPTDKGDSQDFYVFSDDETAQVFYDSGKKVSAVA
                     VVYTGGKNVPTAQAVFGKEVAPKPDGSQFLRVLYPKVNYWVSYSRTAGDTPVTSIMMQ
                     RK"
     gene            25392..26381
                     /locus_tag="VF240_19545"
     CDS             25392..26381
                     /locus_tag="VF240_19545"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7177462.1"
                     /translation="MAEESTSTSPEQRRVADLISSRRDEILNLWITERLESDEFRDEL
                     ISKKELLHQSRQVLEMLAQALRESDGSDFDHPAFADLRAYLNEISHMRAVKGYTPTEN
                     ATYILALRNVVMPLLVEETDTNTDNLVRDSNYFTRLLDKLGLVMVENFIRSREEIIRQ
                     QRADMMELSTPVIKVWDKILTLPIIGTLDSRRAQMMMESLLQKIVETGSTIAILDITG
                     VRTMDTLVANHLIKTVTAARLMGARCILTGVSPAIAQTMVQLGIDLTQITTRAQMSDG
                     IKLALEMIGRTIVPTKALAKMQSNGAGADGVPTLKFAEQSANTTQAENKHRES"
     gene            26466..26897
                     /locus_tag="VF240_19550"
     CDS             26466..26897
                     /locus_tag="VF240_19550"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7177463.1"
                     /translation="MDGQRIPILKVGRVLIVPIQVDMDDQSVIHLQERILSELERTGA
                     RGVLIDISLLEMVDSFIGRVLSDIAAMARIMDARTVVVGMQPAVAITLVELGLELRGV
                     DTVLNVDEGIRLLRGEFGIDDDEGETALGEFSALAEQIHRP"
     gene            26872..27270
                     /locus_tag="VF240_19555"
     CDS             26872..27270
                     /locus_tag="VF240_19555"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX7177464.1"
                     /translation="MQSRSIALEDEHDIAVARGEVRVLAAALGFRLIDQTRLATVASE
                     LARNVVKYARRGRMIAQPTESTSGRHGLRLIFEDNGPGIPDLQAAMRDGFSTGRSLGK
                     GLPGSRRLVDEFDIKSTVGSGTIVTVVRWL"
     gene            27370..28416
                     /locus_tag="VF240_19560"
     CDS             27370..28416
                     /locus_tag="VF240_19560"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177465.1"
                     /translation="MRVFHSQEIKDEAQVGGARRAVQRFAAPLGFSDDELAEIAIVVQ
                     EIGTNAAKYSRVGGRLHWTTPLDGPAGLELFYWDKGPGIFDIEAAVRDGVTTGGGLGT
                     GFGAIRRLTNEFDVYSTLRATTSRLLHARRTTHGTVVLCRKRVAGATAPPPDEGEGRR
                     RIGAWSRCRTGEDVNGDGYFIGRHDGNILLAVVDGLGHGRGARDATAAALGAFEDWAG
                     EPPDEVLAAAHDALRSTRGAVAAAVVIDREGETFHYAGVGNIDVRVFNSPEPVRPVPI
                     NGTLGSRLSALRVWPHRWSEGTTIVMASDGVSASWDIGSYPGLLAKSPQIFAGVLLRD
                     YGRDSDDATALVVR"
     gene            28503..29849
                     /locus_tag="VF240_19565"
     CDS             28503..29849
                     /locus_tag="VF240_19565"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177466.1"
                     /translation="MEGITSTRLGDIYVRREADIIKVRERVRRLARELGFDSTTQIKI
                     TTAVSELTRNIYEYARAGAISCSVAERGSAQIGLQIIARDTGPGIDEKQLRAILRGSY
                     RSPSGMGVGLAGTRRLMDEFEIESKPQEGTRVLVIKWLAAGAGAGIKARVAELREFFA
                     AEVDEAAMEELTRQNRDLVQVLNELEEKREALERLNSQLGESNRELNEANAKLRELAE
                     MKEEFLALTTHDLRSPLTVISGVINFFTSGRLGDMTPEQKNMVAMMERNTHSLIELVN
                     DLLDASKLESGSMRLDLTSVDLRALTSELRDTMEPLARDKEIALEAGLPDGLPLVRAD
                     RAKLRRILVNLVSNAVKFTPKGGRVELSAEPRDGAVRVSVADTGVGIAPEDIQRLFDK
                     YEQARSRATRSEKGTGLGLYITRQLVEMHGGKIEVRSEVGKGSTFSFTLPTENKKK"
     gene            29940..31391
                     /gene="mpl"
                     /locus_tag="VF240_19570"
     CDS             29940..31391
                     /gene="mpl"
                     /locus_tag="VF240_19570"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX7177467.1"
                     /translation="MNYHLIGICGTAMASLAGMLQARGHRVTGSDEHVYPPMSTMLEG
                     LGIPVAVGYRAENLEPTPDCVVVGNAIPRGNAELEAVLNRRLLYRSQAEVVKEEFIRG
                     RRSLVVAGTHGKTTTTSIAAWVLERGGLDPTFLVGGVVQNFGVSFRVTESEYFIIEGD
                     EYDTAYFDKGPKFMHYLPELAVVGNIEFDHADIYKDLDAVKLAFRRLMNLVPGEGRLI
                     AGWDSPDVRAVVAEMGARLHTRLETYGACDDARWQARDIRIDERGETHFRVVRDGAAW
                     AEFRTPMIGDFNVRNCLAVVVAADAWGVERAAIADALATFKSVTRRMQVRGERRGVTV
                     IDDFAHHPTAVRETLRALRQRYRDRRLVAVFEPRSWSSRLAVFQQDYVEALGQADYVV
                     IASVYDQSKAAEKGSVLDTRELAAEVGVAGDCPSHCIEGADEIVRRLAPELRAGDVVA
                     VMSNGGFGGIHEKLLDALGAGREGDTQEEVHHA"
     gene            31388..32569
                     /locus_tag="VF240_19575"
     CDS             31388..32569
                     /locus_tag="VF240_19575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177468.1"
                     /translation="MKTVQLIPRRIAAVAAVVLLALLCARLDPAFAQDLQGGTGVFIK
                     RAFVKVAANARAARRPPPRRGGARPTTPPAPDRSALVDEAIKMGNAEREAQPPRLQDA
                     ERAYQLGARLDPADARPHAWLGDLYFQQQRFTEAEAELRQAVALDPRDAISYVRLSYM
                     FSKLGRFDEADAAARRVQQILPKEYYGYCSMGWSKFRRKSYDEAAAAYRRAIQLSPRT
                     SGLHSDLGLVLLSQSRYAEAADAFRHAIQLDPNSTGALINYGVVLHRLGQLGKAAEAY
                     TQAAAKAPAATQPRSNLGAISYTRDDRAAAKSHWAESVKLGSAYAVDRAGLLILDGKL
                     PEALAQLEQFTQANAENADGWLLLGDVRRARGDAEGARAAYARAAQLAPEYARLPRPK
                     L"
     gene            32954..33451
                     /locus_tag="VF240_19580"
     CDS             32954..33451
                     /locus_tag="VF240_19580"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7177469.1"
                     /translation="MGLWDKMFGRGVEKVQEPDAQQVFQQLRQKYQAALDKGDQERIQ
                     FQNLHVEGGKLYVKGLAPSEEARTSFMNQIEMLRAGADDVVAEITVQQSQSQAAAAGG
                     AAVSSPAVTHKVESGDTLSKLSKEYYGTPDEYMRIFYANRDKLRDPNRINVGQDLVIP
                     PDDNG"
     gene            complement(33715..>34581)
                     /locus_tag="VF240_19585"
     CDS             complement(33715..>34581)
                     /locus_tag="VF240_19585"
                     /inference="COORDINATES: protein motif:HMM:NF042871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HEX7177470.1"
                     /translation="DRYVATVETSKHRFFVLLDRSVLPDNKLVNIALNDAYFLGVLSS
                     RIHVTWALAAGSRLGVGNDPVYVKTACFEKFPFPDATDEQQARIRELGEALDAHRKRQ
                     QAQHPRLTVTEMYNVLEKLRAGESLTDKDRLTHEAGLVSVLRQIHDRLDAAVSDAYGW
                     SAALTDEEILERLVRLNAERAGEERRGLVRWLRPGFQRPAVGVAAGFGEGFEPAAPAK
                     AGEKKQVWPKTLPEQARALRQTLAAQKGVVTPEELARKFTRARVERVEELLRTLVSLG
                     QAREVAAGRYAA"
BASE COUNT         6103 a        11557 c        10903 g         6018 t
ORIGIN      
        1 tcgccaactt caagttcacc aagtgaggcg gaaggccgtc gcaggcgggg ccgcggcccc
       61 gcccgcgacg gcccaagcct tctaccttga ggaaatgatc cgacagataa ggtgttcagt
      121 tcacgcgaaa ccagcccggg cgcctttgat ctggcatctg gctattacac gaacccgttt
      181 taacttaaag gagagaaccc caaatgaata agcttcgctt cgccttcacc gccctggcct
      241 tcatggcctt caccctggcg gtatcctcca gcgcgcaagc gcaggccacc cgcacgtggg
      301 tctccggcgt cggcgacgat gtcaacccgt gcagccgcac cgccccctgc aagacctacg
      361 ccggggccat ctcgaagacg gccaaggacg gcgagatcag cacgctcgat cccggcggtt
      421 tcggcaccat caccaccacc aagtccatca cgattaacgg cggcggcgcg ggccagggct
      481 acggctcgat cctctccgcc ctcgcgccgc agggcgtgct cgtcaacatc accgacgtga
      541 acgacatccg gaagagcgtc aagctcaact ggctcgacat caacggcgcg agcaccggaa
      601 ccgacggcat ccgcatgatc gccggcaaca cgctccacat cgagaacacc ttcattgacg
      661 gcatggcggg cgacggcgtt gatatcaacg tttccgttgg catcgccttc tcgatgaaga
      721 acgtctcgat ccgcaactgc gtgggctccg gcgtcaagat ggcctccacc ggcggggggt
      781 cggtcggctt cagcctcaac aacgtacagg tcagcaagac cggcacgggc attgacgtgc
      841 agaacggcgc gcgcggcacc atcaccgaca ccgtgatcgc gctcaacacc acgggcatct
      901 ccgtcacaga ggcggggaac gacgccgagg ccaacatcgt gaacgcgacc atcaccgggc
      961 acgtcaccgg catcagcgtg ggcgcgggcg cgcgggcgcg catcgcccgc tgcctgatca
     1021 cgcagaacgt gacctctctg acgccggccg cgcaggtgga caccggcaac aacaacacca
     1081 tcatgggcaa caccaacaac acggccccgg ccggcttgcc cttcccgcaa aactaaagcc
     1141 gcccggcacg gctcaattct tagtcccccg acggtcgccc tttggcgcgg tcgtcggggg
     1201 actaactttt tgccccgcgc cccgggagtc cgagacgcct tatcgaggcg gcgaaagtcg
     1261 tcggcccgca gtgatttcct cgtcgggcgc tctgcttgac tagcctgcgg cgcatggcta
     1321 gactgctttt tcttctcagt ccgccggtga tcagcggtca attaccgaac aacgctggat
     1381 cgatctataa aagcctgcgg ggttgtcgtg cgacccttag cggaaacctg aattgatgtt
     1441 aacgaaacca ggcatcgacg cgctcgggcc gaacgccgcc tccgcgccgc cggacgcgtc
     1501 gcccacgccg ccagcgctgc cccgctacga agccctgccg gacgcaccct tagttaccat
     1561 cgagcctggc aagtcgtgga gtgcgctcga tctgcgcgaa ctttgggact accgcgagct
     1621 gctctacttc ctcatctggc gcgacgtgaa agtgcgctac aagcagacgg tgctcggcgc
     1681 ggcctgggcg gttttgcaac cgctctttat gatggcccta ttctcgctcc ttttcgggcg
     1741 cgtcgccggc atcgacacgg gggcggttcc ttatcccctc ttcgccttcg tgggccttgt
     1801 gccgtggact ttcttcgcca acgcagtgac gacgagcagc aatagcctcg tgagcagcac
     1861 gcacctgatc tcgaaggtct actttccgcg cctgctcgtg cccgccgggg cggtggccgc
     1921 gtgcctcttc gacttcgcgc tctcgttcct ggcgcttctc gggatgctcg tctattaccg
     1981 cgtctcgccc acggcgaacc ttctgatgct gcccgcgctc gtcgccttga tggccggctt
     2041 cgcgctgggc gtcgggatgt ggctgtcatc cttaaacgtc aagtaccgcg acgtgcgttt
     2101 cgtgctgccg tttttgattc agctctggct ctttgcctcg tcggtgattc tcccgtcgag
     2161 cgccgtgccc gagcgcctgc gctggctgct gtacctgaac cccatgtcgg gcgtcgtcga
     2221 gggcttccgc tcggcgctgc tcggcttgcc gttcgactgg cgggccatcg gggtcgcggc
     2281 caccgccacg gccgtcgtgc tcgtctatgc cgcctactcc ttccggcgca cggagcgcag
     2341 cttcgccgac atcatctgaa gatcattatg cggccaatcg tcagggttga gaatgtaggc
     2401 aagctctacc agttgggcag ctcgggcgcc gcctacggga cgctccgcga cacgctggcc
     2461 gaggcggtgc gttcgccctt cagccgctgg cgcggcgacg ctggcgcggc gaccagcgag
     2521 cagtcgttct gggcgctgcg cggcgtaagc ttcgacgttc ggcccggcga gatcgtcggc
     2581 atcatcgggc gcaacggggc gggcaaatcg acactgctga aaatcctctc acgcatcacc
     2641 gagccgacga ccgggcggat cgagctttac gggcgcgtcg gctcgctcct cgaagtcggg
     2701 acgggcttcc acccggagct ttcggggcgc gagaacgtct tcctcaacgg cgcgatcctc
     2761 ggcatgggcc gcgacgagat tgagaagaag ttcgacgaga tcgtggcgtt cgccgagatc
     2821 gaaaaattcc tcgacacgcc cgtcaagcgt tactcctccg gcatgtacat ccgcctcgcc
     2881 ttcgccgtgg cggcgcacct cgaatccgaa atcatgatcg tggacgaggt gctggccgtg
     2941 ggcgacgcac agtttcagga gaagtgcctc ggcaagatgg gcgagatcgc caggggcggg
     3001 cgcaccgtgc tgctcgtcag ccacaacgtc ggcatcgtca gctcgctctg cacgagggcc
     3061 gtctacctcg ccgacgggca gttgcgctcg gaggggccgg ccgagaagat tgtcggtgag
     3121 tacctgtcag aggcgctgga gagcaaggtt cacgacctgg agcggctgcg gcccgccggc
     3181 atgggcgagc gcgttaagtt cctcgacatc agcctcgcct cgaccgaggg ctcgcacgtc
     3241 gaggtcggac gcccgctcga gttccttctg acgatccgct ccgaggacga catgaggggc
     3301 ctgagcatcg gggcgagcat cttcaacgtc gtcggcgcct gcgtcgggtc gctcatcacg
     3361 cgcgaggagt tttcagtgcg ggctggggag gtcgtgtgcc tgcgtctgaa agtctcagcc
     3421 ctcagtctcg cgcccggctc ttactactcc ggcttcagta tcgggcgcgg cgggcatgag
     3481 gtcaggcgcg ccgacctgga tgcggtgctc ggcgttccct ctttccaagt gctgcctatc
     3541 tcggacaact atctcttcac caactggcaa ctcggctggg gcagcgtcat cttcaaggac
     3601 acactattga cggtcgtggg cgacgtggaa gtctaactga cgtagaaacg aggctgggcc
     3661 gggacgaaga cgaagacctg cctttgctgt ccgcctccaa cacatttgtg caaccttgga
     3721 agtcaagata gtctgtggta tacgatacag cccgggtcgg gtgctgaatc tttcgggtcg
     3781 cccgcttcac gaacccatca ccatgaccca ttctttaaca gtcgagatga gggaagagcg
     3841 ttcaatgaat cagacgcttt cgtttgtttt agacccggtg aagctcgacg agctagccga
     3901 aaagtaccgt gaggcgtacg cgcaagccgc ccccttcccg cacatcgtca tcgatgattt
     3961 tttaccggcg gacgctttga ggcaggtact ctcagagttc cccggcgcgc ggcagattga
     4021 ctggaataag ttcaacgatc cggcgcagcg gaagctgggg tcaaccgccg agtcgcaaat
     4081 cggcgagtac acgcgtttcc tgctatacca gttcaactcc tcgaccttta tcaggttcct
     4141 cgaaaagctg acgggcatta gcggcttgtt gcccgatccc cactttatcg gcggcggcat
     4201 acaccagata ttgaggggcg gctttctgaa aatccacgcc gacttcaacc gtcacccctt
     4261 gctgccactg gatcgtcggc tgaacctcct gctctacctc aacgaagatt ggagggaaga
     4321 atatggggga catctggagc tttggagcag ggacatgtcg gagtgtcggg cgcggatact
     4381 ccccgtcttc aaccgctgcg tgatcttctc gactacggac tactcgttcc acgggcaccc
     4441 cgacccgctg aagtgccccc aggaacggac gcggaaatcg atggcgctct actactactc
     4501 taacggacgc cccgaagagg aaagctcccc cgagcacggc accctcttcc agcaccggcc
     4561 cggcgagtcg tacgacctga gcgcggtcgt atccccaccg caaccgctca ccgccaagca
     4621 aatcatcata aagtttgtcc cccctatcgt gatggaggct tacctgtcct tgaagcggag
     4681 ggtgtaatgc cccgtgtgag aggtcgtgcc tgaaaagtcc gctcgtccca caacctctca
     4741 acccgctcga caattccgcc tgtcaatcga atttacagca cacacggacg aggctggacg
     4801 gggagattcc tcgcctcaaa cctgccgccg ccgacagtgg ccccgcgccg gttgatttta
     4861 cgcacacaat acggataatg cggattggct tcatagtgta ggagttcatg tcatttaaaa
     4921 taggagtgaa acgtgaagat gcgtagcatt aaaagctttg tcccctacag ggtgcaaaat
     4981 gccatgagag tcttgctggg tatctcggca attgaggcga ggatgagcga actcgaagag
     5041 cgtctcggtc gctccgagga taagctcgac gaattcgaaa agatttcctt tagcaaagat
     5101 ttttgggaaa ggtcgcgggt acgctggcgc ggaaacgagc cgtcaaccgg cctaacctgg
     5161 aataaagaat tgaaaggtga caacttcatc tcgaaggtcg cctcctacaa tgctttcggc
     5221 cctgacagcg ccgtgcttga aatcgggccg gggtacgggc ggcttttgaa agcgatgctg
     5281 acacagggcg tgtcctttaa gaattatttg ggcgtcgatc tgtcaccgga cagagtgcgg
     5341 cagcttaaag aaacctttgc gaatgagaag attgacttta gacaggggga cgttgagaac
     5401 gtggcgctcg atgggacctt cgacgtcgtc atctcttctc ttactttcaa acacctctat
     5461 ccaacttttg agaagtcgtt ggctaatctc acgacttacg tcaacctcaa cgggatgttg
     5521 ttcttcgacc tcgtcgaggg taaggcaaac ttctttgaat acggcggtga aacgtacctc
     5581 catttttatc agaaatcgga ggtgctggag atactaggcc gcactaatct tgagctggtc
     5641 gccttcgatc aggtcgagca cgacccggag cacgtgcggc tcttagtagt agcgaaaaaa
     5701 gtaaagtaga ctttacgtcg tcgcgccttt atgactttgt cggctgctgt gaggaacaag
     5761 ggccaaagcc agacgagaaa ttaatgagct aattcagccc gcgcgcctcg ccacctcgac
     5821 ctggatcgag cacgcgccgt tcgcgatgta tttagttgac gtgctgtgag cgcgtgtctt
     5881 cgtcgagctg ggggctgact gcggcatctc ctactaattc ctctgtcaag cggcgaatga
     5941 gtcaggactc cgcgcgccta ttggcggccc ttcttttcag gcgccgccga tctcaagtgt
     6001 cggcgatcac gtcgccaacc ggcacttgag ttggggcagc atcgtgttca gagacacact
     6061 tttgacggtt tgacggccga ggggccgtag gccagcccag cgtggaatcc atccgcaacc
     6121 tccaaagaaa gctcggcggc gtcggttacg cgacgcgggt gaagctggca acccttctgc
     6181 tgctcgcgct gacggccctg ctcaccaacc tcatctacct gcgtgagaac cgagggttag
     6241 agccgacgac ggccgggcac gacgacatca atatttatga gcgtcggttc ggtgtgctgc
     6301 gcgaggcgct gcccgcgcgg ggcgtcgtcg ggtacttgag cgacgagcag gaagtgaacg
     6361 aggccacgaa aaaatattac ctgacgcaat acacgctcgc ccctctgatc gtcgtcaggg
     6421 acgctgacca ggcgctggtc gtcggcgact tcgcgcgcgc aagggagggg gcgccggaga
     6481 ccgtcgccgc tcagctcagg ctcatcaagg acttcggcga cggcgtcgcg ctctacgcgc
     6541 gggaggagcg ttgatgactg ccgtcctctt cgcgctctct tcgctcgtcg cgctggcctt
     6601 cgggtttctt ttcgtctctc ttctctggcc ggcggggcgg cccttcgggg agcgcctgct
     6661 tctgaagctc tgtctggcgg cgggcttcgg gtacggactc acgtcgtgcc tctgcttcgt
     6721 atggctactg atgcacgggc gggccgaccg cacttacctc ttgttcgagg ccgtcgtact
     6781 cgcgcttctg cttgcgggcg tcctcttcag gctgagccgg gcgcgaggac gctcgactgt
     6841 cgccgaggga gagagagcga gagagagcgc gagagcgcga gcgagagagc gcgagagcga
     6901 tgcgccgccc gagaggtggg gatggctttt cacggcgttc ttcggcgtct cgctcgcctc
     6961 gacggcgctc gccgtggccg ccgctatcgt gcgcgagccg cacgggggcg gctgggacgc
     7021 ctgggggatt tacggcctgc gcgcgcgcgc aatcttccgc ggcggggaga actggcgcga
     7081 aggcttctcg gcgctcactc cctggtctca cccggattac ccgctgctgc tgccgttgag
     7141 tgacgtgcgg agttggctct actgcggggg cgaggtggct tacgctcagt cagtcctgag
     7201 cgcaattttc accctggcca cggccggatt attatactcg gcgctcgcgg cgttgcgcgg
     7261 ccgcgcgcaa ggttatctgg ccggcgtggt gctgatgggg ttcacgacct tcatcgtcta
     7321 tggaagctcg gcctacgccg acgtgccggt catgttcttc ttcacggcga cgctcgcgct
     7381 ctttgcccta cacttcgggt caggcgaggg gtcgcgccaa ttcctgattc tcgcgggggt
     7441 ggcggcggga ctggcggcat ggacgaagaa cgagggactg ctcttcgtcg cggccgtgct
     7501 ggccgctcat ctggcggtga cagccttcgc cggggggcgg aagacttacg cgcggcagct
     7561 tttcttcgtc gcgctcggcc tgctgccggt gttggccgtg gtcgtctact ttaagacgtg
     7621 ggtcgcgccg ccgagtgagt tgacggcggc gatgggcagc agctcggccc tcggcaagat
     7681 attcgactat cacaggtacg ttcacatcgt tcgggaattt ttattgcagc tcctctatta
     7741 tcgcgggcgc ggcgtcaatc tcacctacct gctcgcgatc tatttagtgt gcgccggggt
     7801 gagtcgcaag cacagatcgg gcgtcatcca cgtggggctg acgctgtgcc tgatgttggc
     7861 gggatacttc tgggtctatc tgacgacgcc ctacgagctt gtctttcacg tcaggttttc
     7921 gatggaccgt ctgctgctcc aactgtggcc gggcttcctt ctcctgtact tcctggccgc
     7981 gaccgccccc gaggaggctt tcggccaagc ggcgtcaggg ggaaggctcc gggcaagttc
     8041 gggggtcgca gcctgaaggg ttatgagcac gaacatgaac aacgaaccat gcctgtccgt
     8101 cgtcgttccc gcctacaacg aggaggcgac gctggaggag gtcatcctca aggtgctggc
     8161 actcccgcag ttgctcgaag tcgtcgtggt ggacgactgc tcgagcgacg gcacgcccga
     8221 ggtcacgcgc cgtctggccg acgcctaccc gcgagtgcgc gtcgcgcgcc acgagcgtaa
     8281 cgccggcaag accgaggcgc tcaagaccgg attcgcgctg acgagaggcg ggatcgtcat
     8341 cgtgcaggac gccgatctcg agtacgatcc gaccgagatt cccgccgtca tccagccgat
     8401 catcgagggc cacgccgatg tcgtctatgg ctcgcgcttc atggtgcggc gcgcggcccg
     8461 cgtgctctac tactatcact tcctggccaa caagttcctg acctcattct cgaacgtgat
     8521 gacgaatctc aacatgacgg acgtggagac tggatacaaa gccttccgcg gcgagatcat
     8581 ccgcaacatg gtcatcacct ccagcgggtt cggcttcgag atcgaggtca cggcgaagat
     8641 cgccaagctg aagtgcgcca tctatgaggt tcccatcagc tactacggcc gcacctacga
     8701 ggaaggcaag aagatcgggc tgagcgacgg cttcgccgcg ctctggttcg tcgtgcgttt
     8761 caacctcttc tgcggcctcg acgcctcctt ccgcagccgc cccgcgccgc gcggcgagca
     8821 ggtgtgcgcg cccgacccgg cgcggaactc gggagccttg aggtgatgag cgaaaagatc
     8881 gtctacgtcg gccgggactt ggaagcgatg tccttcgccg tcaactacca ccgctggatt
     8941 ctcgacgcct tcggcccccg cctgggcgcg cgcctcgtcg aggtcggggc cggctcgggc
     9001 gagttctccg aactccttct ggcgcgcgag ccagagacga tctcgctggt cgaaccctcg
     9061 gccgacatgc accgcctact cgaagctcgc gtccacgccg cgggcgcggg cgcgaacgtc
     9121 gccacctaca acgccacctt cgccgacgta gccgagcgca tccgcgcgga gcagcgtccc
     9181 gactcggtca tctacgttaa cgtcctcgaa cacgtccaag acgacgaggc ggagttgcga
     9241 gccgtccagc gaacgctcgg cgcgggcgga agaatcttca tcttcgtgcc cgcgttgccg
     9301 tggctctacg gggagttcga ccggagggtc ggccattacc gacggtacac gaaggccgag
     9361 cttgaagaaa aatgtcagcg cgcggggttc aggatgttga agtcggtcta cttcgatctg
     9421 gccggcatcg cgccgtggtg gattcagtat cgcctgctca gggccagcac tttgagcccc
     9481 ggcgcagtca agctgtacga caggctggtg gttcccgtca cgcgggccgt cgagtcgctc
     9541 atcaacccgc ctctcggtaa gaacctgctc ctgatcgcgg agaagactta gatggccagc
     9601 cgcgccgggg cgagggccgc gcggttcgcg gcgagtcgcg gctggggctg gagctggagg
     9661 gcttgctggt ttgaaatccc cggccttacc cggtagtatt tgctggacaa ccaactccct
     9721 cggcgataaa taaacgcaca cagaagattt aatgcccgac ttcaacccgc tcgaccatct
     9781 catctgtctc caacacccgg cacgcctcgc cccctcaacg tggatcgagc acgtgccgtt
     9841 tgcgatgtat ttgatcgaca tcctgcggcc gagggtcttc gtcgagttgg ggacgttcta
     9901 cggcgtctcg tactgcgcct tctgtcaggc ggtcgaagaa ctgggggccg gcacacggtg
     9961 ctacgccgtt gacacctggc agggggaccc gcaggccggg ttcttcgccg atgaggtgct
    10021 caaccacctg agggagcatc acgacccgct ttacggcggc ttctcccgcc tgattcagag
    10081 cgacttcgac gaagcggcag ggcaattccc cgaagggtcg gttgacctcc tgcacatcga
    10141 cggcttccac acctacgagg ccgtccgcca tgactttgag acgtggctgc cgaagatgag
    10201 cgagcgcggc gtcgttctgt tccacgacat tagcgtgcgc aacgagggtt tcggcgtctg
    10261 gaaattctgg gacgaagtga aggccggacg cccgcactac gaggtggatt tcggatgggg
    10321 actcggcctc atcgccacgg gacgtgagcg cccggcagct ctcagcgttc tgttcgactc
    10381 gccggatgat gagctgcggc gcatccgcga atattttcac cggctcggcc tcgggctgga
    10441 agccgcgcag gaattacact cactcaaaaa ggcgacgcgc gcgcgcgacg aggcgagccg
    10501 cgcgcgcgag gagcgcatgc ggcgcaacca cccgctgctc acgcgcgcga gcaatctcct
    10561 gcaagtctgc gccgagcatg ggctgggcgc cgcactgcgc gccgggcagg agaagattcg
    10621 tcgaaagggc gaggcgtcgg ggcggagcgc gggcgaggat gccgcgacgg ccgcgaactc
    10681 ttgagagagc aagtgaagac gatggaagcg ccgccgaagg tttcggtcat cacgccgctc
    10741 tacaactcgt cggcattcat ccgcgctacg ctcgacagcc tgcgcgcgca gacctacgag
    10801 gactgggaat caatcctcgt cgatgacggc tcgacggacg acacggctga ggtcgtccgc
    10861 ccctatcttg aggacgcgcg gtttcgtctc atacgtcagg agaatcgagg catcgcgggg
    10921 gccaggaaca cggcgattca ggcggcgtcg ggcaaatggg tctgcctgct cgaccacgac
    10981 gaccgctggc tcccgcgcaa actcgaaaag cagattgctt tcgcgcgcga gcattccttc
    11041 gacatcacct gcacggacgc tttcatcgtc acggagaacg agcgttgggt ctatagccgc
    11101 ggattccccg aagtggcggc ggaggtgcag cgcgccgcgc acgatccaag cgttgatgtt
    11161 ttcgggctgc tcatcaaact cgatttcctg tgcgcgtcgt ccgtgatgat ccgccgcacg
    11221 ctcttcgacg agcgcgggct gctcgatccg tcggcggttc cggcggacga ctacgagatg
    11281 tggctgcgtt gtctgccaga ggcgaagctc ggctttctcg gtgagcctct catcgagtac
    11341 atggtgcatg cgaacaacta ctcgcgcgac gagccgcgca tgatagagca ggccatacgc
    11401 gtgcttcagc tccacagaaa gcggcacgcg ctcagcgcct tgcgtcggcg acagttcgac
    11461 gaatcgctgg cctaccagtt cggcctgctc tttaaaaagc tctcactgga tcgctcaaag
    11521 gcttacgcgc tcacgcgctc gctctcgctc atgaaagacg ggccgggcgc gcttcgcctg
    11581 ctccgcttcg ctgtgtggag gccgcttgcg gagcgcgccc gccatgcggc gcgttaccgt
    11641 ctgggcctcg cgccccgaaa agtctgaacg gtcagagatt gcttaattgc ggattctctt
    11701 cgtagcaatg gcgaacagca tccacactgc tcgctggatc agtcagttgg aggggcaggg
    11761 atgggacgtg catctcttcc cggcggaaga agtatgcccg cacccgcgcc tgcggaactt
    11821 gaagctgcac gagccgttga gcgtcaggcc gccggggctt gacccgagcg tggaggtcat
    11881 cggcgaatgg ccgctcccga agggcgcatc gctcgcgcgc cgcgtcgcgg ggaagttcgc
    11941 tccctcgtgg ccagaccgcg cgtggcatct ggcgcgcacg atccgaaagc tccggccaga
    12001 catcgtccac tctcttgaga ttcagcacgc gggctacctg actctcggcg cgcgcgcgca
    12061 ctttgccgcc gacgactttc cgacgtgggt cgtctcgaac tgggggagcg acatttatct
    12121 tttcgggcga ctggccgcgc acgccgggaa gatcagggag atgatggcgg cctgcgacta
    12181 ctaccactgc gagtgccgcc gggacgtggc gctggcgcgc gagttcgggt tcgagggcga
    12241 ggtcatgccg gtcttccccg tgacgggcgg cttcgaggtc gagcggatgc gcgcactacg
    12301 gcagccgggg ccgacctcct cgaagcgccg tgtggtattg aagggttatc agaactgggc
    12361 ggggcgcgcg ctggttggct tgcgcgccat cgagctttgc gccgacctga tgcgtggata
    12421 ccgcgtgacg gtgtatctgg cgcagcccga cgtgatgctc gccgccgaac tcctcagtca
    12481 gaagaccggc atccccatcg agtgcgaatc gggggagtgg acgcacgatg acgttctgcg
    12541 gatgcacggc agcgcgcgca cctcgattgg attgagcatc agcgacgcca tcagcacctc
    12601 cctgttggag gcgatgatca tggggtcgtt ccctatccag tcgcacacgg gctgcggcga
    12661 cgaatgggtg aagagcggcg agaacggcct gctcgtccac ccagagtcgc cggaggaagt
    12721 ggccgccgcg ctcaggcgcg cgctcacgga tgacgcgctc gtggatcgcg ccgccgaaat
    12781 caacgcgcgg ctgaccgccg agcggctcga caagacgctg atcgccgcga tgaccgttga
    12841 gatgtacaaa aaaatcgctt cgccgcggac aagcgcgcgg taagagcggc ggggcggcaa
    12901 cgaccggcgg gcgctgaggc cacaatctcc gatgtctaac ctgacagcaa ttcttctgtg
    12961 cggcggcaag ggcgagcggc tccggccctt caccgacacg gcgccgaaga cgctggtgcc
    13021 gctgggcggc cggccgctgc ttcatcacct gctgtcgtat cttgcggcgg agggcgtctc
    13081 gcgcttcgtg gcctgcgtcg gctacaaggc cgaggccgtc gaagagtttt tgcgcgagcg
    13141 gcgcgacccg tcgtgggagg tcgagtgcgt caactcgggc gacgcgagca tgacggatcg
    13201 catcctcgac gcgagggcgc atgtcggcgg tcgcgccctg atctgttatg gcgacacgct
    13261 ggcgaacgtt gatatcgggg agttgctcgc gagccaccgt gcgagcggcg cgctggcgac
    13321 gctcacgacc tacccgctgc acagtccctt cggcatcgtt cacttcgatg ggtcggggcg
    13381 cgtctcggag tttgccgaga agccggcgct gccgtactgg atcaacatcg gcttcattct
    13441 ctgcgagccg gaggccttcg cgtatcttct gcccggctcg gacatgccca ccttcctctc
    13501 ggcgctggcc gcggcgggca ggctctatgc gtacaggcac actggcaagc acctgaccgt
    13561 caacaccgag aaggagcgag ccgacgccga agtcgagatg gtcgaatttt tcacttacat
    13621 ggatgggcaa cagagatgaa taaaaagcgc gttttggtga ccggaggatc gggctttatc
    13681 ggctcgcatc tggtacaccg cctgctcgac gacggggcca aagtcgccgt cacggtgcgt
    13741 tacgggaacg tcatgaagaa cgagcggctc cgcggctgct gggacgacct cgaggtcatc
    13801 gaggccgacc tccgcaaccg aggcgcgctg gcctgcatcc gcgacttcgc ccccgaagta
    13861 gtcttccatc tggccgccta caatcacgtc ggccagagct tcgtgcaggt cgaagagtgt
    13921 ttcgacgtga acgcgaaggg aagcgctaac ctgctcgaca catgcggcgg cgcggcgagg
    13981 ttcgtctaca tgtccacctc ggaagtctac gggcatcaga ccgaggttcc gttcgtcgag
    14041 acgatggacc cggagccgat ctctccttac gccatcacga agtacgcggg cgagctgtac
    14101 tgccgcatga agcagcgcat cggcggcgac acctcggtcg tcattctccg acccttcaac
    14161 accttcggcc cctatcaaag cgcgaaggcc gtcatcccgg agctgatcgt caactgcctg
    14221 aagggcgagc ctatcaggac gacgaagggc gagcagacgc gcgagttcaa ctacgtctcc
    14281 aatatcgttg acggcctcgt cgcggcggga agttacgagg gcaaacttga cggcgtgctc
    14341 aacctcgcct cgggcgagga ggtcgccatt cacgatctgg tcagaaagat cgcggagctg
    14401 acggagacgc gctcggagat tgaaatcgga gccttgccct accgcccgac cgaaatctgg
    14461 cgcatgtacg ccgacagcgc gcgcgcgcgt cagacgctcg gctgggagcc gaaggtgggg
    14521 cttgacgagg ggctgcgcct gacggtcgag tggttccgcg agtacagacg cggggattcc
    14581 gacgcgtgag caactccatt gaagaaaaga tgagggttct gattctcggc gggtcgggga
    14641 tgctggggca caagctctgg cagactctat cggagcgctt cgacacgcgc gcgacgttcc
    14701 gccagtcgcc agccgcctac gctcgatacg gaattttcga tccggcgcgc tccatcggtc
    14761 acgtctcggc gcaggacttc gatggcgtcg tgcgcgcgct cgccgccgtg cgtcccgacg
    14821 tggtcgtcaa ctgcgtcggc atcgtcaagc aggacgcggc ggcgaaagac ccgtatcaga
    14881 gcatcaacac gaacgcgctc ttcccgcacc gtctggcgca ggcgtgcatc gcgtcgggcg
    14941 cgcggctcat ccacatcagc accgactgcg tcttcacggg gcggcgcggc aactacaaag
    15001 agacggacgc ggcggacgcc gaagacattt acgggcgcac gaagtggctc ggcgaggtgg
    15061 actccgagcg ttgcctgacc atacgcacct cgatgatcgg gcgcgagctg caaggcgcgc
    15121 acggcctcgt cgaatggttc ctctcacagc gcggccaacg cgtgcgcggc ttcaagcgcg
    15181 ccgtcttcag cggcttcacg accgaagcgc tggcctccat catcggtgat gtgatcgccg
    15241 gccacgcggg cttgcgcggc gtctggcacg tcgccgccga gccggtcaac aagttcgacc
    15301 tgctcactct ggttaaagag gcttacgggt tggacatcga gatcgagccg gacgaaactt
    15361 tcgtctgcga ccgcagcctc gacggcgcgc ggttccgaca agcgacgggc ttcgcgccgc
    15421 cgtcgtggcc ggagatgatc gagcggatgc gcggcgacgc gaccccctac gaggagtttc
    15481 ggaggattca tgctcaagga taagagagtg gtggtgacgg gcggcacagg ctcgctcgga
    15541 aaagttctgg tgcggcggct gctgtcgggc gaggtgggct tgccgcgcca gatcgtcgtc
    15601 ttctcgcgcg acgaggccaa gcagcactcg atgcgtctgg ccttccagca cctgcgcacc
    15661 gccaccgacg aagtcatcta tcacaacttc gagcagctcc tgcaattccg catcggcgac
    15721 gtgcgcgacc ccgcgagcgt caacgccgtc ctgcgtcacg ccgacgtggt cttcaacgcc
    15781 gcggccttaa agcaggtgcc ctcctgcgaa tacttcccct tcgaggccgt gcagaccaac
    15841 atcgtcggcg cggagaacat cgtccgggcc gtgcgcgagg gccgcctccc cgtcgagacc
    15901 gtggtcggca tttcgacgga caaggcgtgc aagcccgtca acgtcatggg catgacgaag
    15961 gcggtgcagg agcgcctgtt cgtctgcgct aacctcgacg ttcccgacac gcgcttcgtc
    16021 tgcgtccgct acggcaacgt gctcgcctcg cgcggctccg tcatcccgct ctttcacgag
    16081 cagatcagac gcggcggccc cgtcaccatc acgacggccg acatgacgcg cttcctcctc
    16141 agtctggagc aggccgtcga taccgtcttc gcggcgctcg gcgaggccga acgcggcgag
    16201 acctacatcc cgcgcgtgcc ctcggccaga gtgaccgacg tgtcggatgc gctcatcggc
    16261 gaccagaaga ttaagacggt cgtcaccggc atccgccccg gcgagaaggt acacgagata
    16321 ctggtctccg aggaggaggc ttttcgcacg gtcgcgcgcg gcgactatta cgtcatccag
    16381 ccgatgctgc cggagctacg ccagagcgag gagatcaaaa acgccctcgg ccgcgaatac
    16441 agctccgccg acaacgtgat gagtccggcg gaggtggccg aactgctcaa gcggcacaag
    16501 ctgatgccca gcgaccgctt cgtctatgag gaagacatgc tcgcatgaag gtgatgacca
    16561 ttctgggcac gcggcccgaa attatccgcc tcagccgcgt cgtcgaaaaa ctcgaccgcc
    16621 tctgccaaca cgtcctcgtc cacacggggc agaacttcga cgccgcctta agcgacctct
    16681 tcttcgagga gctgggcgtg cgccgtcccg accactacct cggcgtgcgc ggtgacacat
    16741 tcggcgaaca gatcggccgc atcatcagcg agagcgagcg cgtgatgacg gccgagcgtc
    16801 ctgatcgcct gctcatcctc ggcgacacca acagcgcgct ttcggccctc gtcgccaagc
    16861 gactgggcat ccccgtctat cacatggagg ccggcaaccg ctgctacgac gaccgcgtgc
    16921 cggaggaagt taaccgccgc gtcgtcgatc acagcagcga cgtgttgatg ccctacactg
    16981 agcgcagccg gcagaacctc ctgcgcgaag gcttcgcggg cgagcgcgtc tacgtcaccg
    17041 gcaatcccat cctcgaagtc atcgagcact acaatgagcg cgtgcgcgcc tccgacgtgc
    17101 tcgcgcggct ctccctcgaa gcgcaaaaat attttctggt gacgatgcac cgcgccgaga
    17161 acgtggacgt tgaggagcgc ctgcgctcgc tcatcggcgc gctcgaagct ctggggcgcg
    17221 aatatggcct gcccgtcgtc atcagcacgc acccgcgcac gcgcgccgtg atggagcgct
    17281 tccgcgtcga tgccggggcg cacgccgagc tacagtttca cgcgcccttc ggcctcttcg
    17341 atttcgtcgc gctcgaacag aacgccttct gcgtcctctc ggactctggc acggtgcagg
    17401 aggagtgttg catcttcggg gtggccaacg tcaccatccg cgacgtgacc gagcggcccg
    17461 agaccatcga gtcggggagc aacatcctga gcggcgcgga gccggaggcg gtgctgcgct
    17521 gcgtgcgcgc cgtgctcgac ggcaaaagcg aatgggcgcc gccgcccgag tatctcgtca
    17581 aaaacgtcag cggcacggtg gcccgcatcg tgctcggtta cagacgcgaa ggccgggggt
    17641 gagtaggccg gggaacacgg atgagacgga taaggacgga ttatcacaga tcaatccgtg
    17701 acgacccgtc ttccgtccgt ctcatccgta ttctactcct ttaagatgtg tgttgaccgg
    17761 ccccgcgcgg ccgacgacga agtatgaaga tcgcgctcgt ctcagaagcc ctgccgccct
    17821 cggcctccgg tcagtcgatg gtgctctccc gcctgctcgg cggtcttgac cccgccgact
    17881 actgcctcat ttctaatctc aactacgacg ccgccgcgcc gcacaacgcc tacacgcaga
    17941 gattgcccgt caactatcac cacctgagcg cgccccgaca gatcacgcgc ggcagccgct
    18001 tcggtctcat ccacctgcgc cacggcctca acttccccct gaccgtcgcg cagtggggcc
    18061 ggcgcatcgc cgacatcgtc cgccgcgagc gttgcgaagc ggtcgtcgcc tgcaccggcg
    18121 aggtctcact cctgcccgcg ggctacctcg gtgcgcgtct cgcgggcgtg cccttcttcg
    18181 catacgtctt cgaccactac tcctatcgcg agtgggccaa cccggccgcg cgcttctggg
    18241 cgcggcgtct cgaagcgcgg ctcctgcgcg gcgcggccgg cgtcatcgtc ccgaacgaga
    18301 ttttgcgcga cgacctgcgc cgcaggtgcg gcgtcgaatc ggaagtcatc cacaactctt
    18361 tcgacatctc cgactacgag tcggccccgc cgcctccgcc gtcgcctaac ggcgaggtga
    18421 agatcgtcta cacgggcgac gtgtacgagg cccactacga cgccttccag aatcttctgg
    18481 cggcgctccg cctgctcggg cgcgacgacg tgcgtctgca cctctacacc tcgcgcacgc
    18541 ccgaggagat ggccgcgcgg ggcatcagcg gccccgtccg catccacccg caccgggcgc
    18601 tcgccgagat gccgcgcgtg cagcaggagg ccgacatcct cttcctcgcc ttggccttcg
    18661 actcgcccta ccccgacctc gtcaagacct cggcgacgat gaagctcggc gaatttctcg
    18721 ccactcgccg gcccgtcatc gtccacgcgc cgcccgactc cttcatctcg tggtacttcc
    18781 gcacgcacga gtgcggcgtc gtcgttgacc gccgcgaccc cgagctgctg gcgcgaaaaa
    18841 tcacagagct tctggccgac cccgagctgc aacgccggct cacggagcgc gcctggggcc
    18901 gcgcccgcgc cgacttcagc atcgcgggcg cgcgcgctaa gtttctcgat gtcttgagac
    18961 gcggcgtccg cgcccgcgcg ccggagcagg agccgcgccc atgacgccgg acgaagcagc
    19021 ggcggcgacg ccgccgcggg ccgcgcccct cgtcagcgtc gtcatgccga cctacaacta
    19081 cgcgcgctac gtcggcgacg ccctcaggag tcttcaggcg cagacccacg cgcgctggga
    19141 gtgcgtggtc gtggacgacg gctcgaccga cgacaccgag gaggtcgtcg ccggcttcgc
    19201 ggcgcgcgac gcgcgcatca ggtacgtgcg tcaggagaac aggcggcagg cggcggcgag
    19261 gaacaatggg ctgcggcact gcgccggcga ctacgtgcag tttctcgacg ccgacgacct
    19321 gctggagtcg cgcaagctgg agcggcaggc ggccgatcta gaggcgcacc ccgaggtgga
    19381 catcgtctac ggcggcgcgc gcggtttcac gtcggccggt cagagcggcg agggcgcggc
    19441 gttggacgac agtcccctgc ccgcgatgcc cgaggcgtcg ggcggcgggc gcgagttggc
    19501 gccggggctt gtgcgccgga acatcatgtt cgtcaactcg ccgctggtgc ggcgtcgtct
    19561 cattgaggag gtcggctcct tcgacgagcg cctgccgccg gccgaggact gggactactg
    19621 gctgcgctgc gccctcaagg gcgcgcgctt ccaattcgtc cctgaggagg gcacgctggc
    19681 gctcgtgcgc acgcacccgg taagctccag ccgcaaccgc gccgtggctt acgcctccct
    19741 gctccgcatg cgcggcaagc tcgcggggct gacggacgac gccgaactcc tcgcgctcaa
    19801 ccgcgagctg gcggcggccg acgagggata cctcggcgtc gaggaggccg cggcgggccg
    19861 tgtgctcaag ggcgtgctcc acctcgggcg ggcggcgctg cgcgtgcgcc gggcgcgctg
    19921 gcgggcgaag tggcttttgt gcgcgctctc ggcgcccttc gtctcgccgc cgacgctcag
    19981 gaagctggcg actgaatcga tcacgcgacg ccgcgcgcga acaacgtagc gaccgccccg
    20041 aaacaaacat gattatcaga gacacgaaac tcgcaggcgc tttcctcgtc gagccggagc
    20101 tgcgcgagga cgagcgcggg ttcttcacgc acgtctggac gcgccgggag ctggcggagc
    20161 gcggcgtcgc gtgtgatttc gccgagtgca acgcctcctt cagccggcgg cggggcaccc
    20221 tgcgcggaat gcactatcag gccgcgccgc acgggcaggc caagctggtg cgctgcacgc
    20281 acggctccat ctacgatgtc ggcatcgacc tgcgccccga ctctcccacc tttcgccagt
    20341 gggtcggctt cgagctgtcg gcgcgcaacc gcctgatgct ctacctgccg gccgagttcg
    20401 cccacggctt ccagaccctc gaagacgaca cggaagtcct ctacatgacc tcagcccctt
    20461 acgcgcccga cagcgggcgg ggcgtgcgct gggacgaccc ggcgttcggc gtccgctggc
    20521 ccgaagtcgg cgagcgcacc ctgatcgcga gggatcgcga gtacccggac ttcgccgcgt
    20581 gagccgcccc gctggagccg agatggacac tccgcgaatc accgtcgtca cgccctcttt
    20641 caatcagggg cagtttatcg aagagacgat cctgtccgtc atcgggcagc agtaccccaa
    20701 tctcgaatac atcatcatcg acggcgggag cacggacgcg agcgtcgaag tcatcaaaaa
    20761 gtacgagcag catctcgcct actgggtgag cgagcctgac cgcggtcagg cccacgccat
    20821 caacaagggc ttcgccaaag cgaccggccg tattctcgcg tggctcaact ccgacgacat
    20881 gtacctgccc ggcgcgttcg ccttcggcgt ctccgcgctg aaggcggacg ccgcgcccgg
    20941 actgctcttc ggcaactgtc tgcacttcgt cgagggcgat acgcaagctt acggcagcga
    21001 cgtgcgcgcg gggcacgaga cgatgaacct gacgctcgcc gactacatca tccagccgag
    21061 caccttctgg acgcgctccg cgtgggaggc cacggggccg ctcgacgagt ctctgaactt
    21121 cggcttcgac tgggagtggt tcatccgcgc ccgcaaggcc ggggtcgcct tcgtccccgc
    21181 cgacaagtat ctttcgctct accgcatcca cggcgcgcac aagaccggca ccggcggcga
    21241 gcggcgctta caggagctgg ccacgatcta cggccggcac gccggcgcga agtacgaaag
    21301 actcttcgcg cgctgcatgg cctcgcgctc gaagatcgtc gcgggcagga cactgctcgg
    21361 gcgtctgagg ctttcgcgtt tcgaggcggc ggcgctcaag ctccgctttc ccggcctatt
    21421 ccgcggcttc gagcccgaag agattagcga tatgatgacc atgctttgaa gaagtgataa
    21481 gtgatgagtg atgagtgata agagctgtta gctattagct gttagctgtt agctgttagc
    21541 caatagccaa gtctctttct tatcactcat cacttatcac tcatcacttt gttttctcca
    21601 tgcgacccaa gatcagcatc gtcactccgt ccttcaatca ggccgcgtac ctcgaacaga
    21661 ccatcctgtc cgtcgtcggg cagaactatg agccgctgga gttcatcatt attgatggcg
    21721 ggagtaccga cgacagcgtc gaagtcatca aaaagtatga gcagcatctc gcccactggg
    21781 tgagcgagcc tgaccgcggc caggcccacg ccatcaacaa gggcctggcg cacgccaccg
    21841 gcgacctcgt cgcctacatc aactccgacg acctctacct gcccggcgcc ttcgccgccg
    21901 tcacgcgcca cttcgacgag cacccgcgct gcgagtggct gtgcggcgac acgctgatgt
    21961 tcggcgaggg cggcgttgac cagacctcac tcgtctcagc caacgtgccg cgcacggccg
    22021 ctcaagtgct ctcctgggct tacaaggccc cgcagcccgg aatgttctgg cggcgcgaat
    22081 tgttgcgcga cggcttcgcc gagcagtggc gctactgctt cgaccacgag ttgtacgtcc
    22141 gcctgctgct cgacggtcac gcgtgcgagc atctgccgct gcccgtggcc gcctaccgcc
    22201 tgcacgcgac gagcaagacc gtggccgagg gcgacctctt cgaccgcgag ttcgacgaca
    22261 tcgccgacct gtacgagccg cgtctgcgcg gggcgggccg gcgctggtgc gccgccacgc
    22321 gccacctccg acactccttc gccgccagcc gcaggggcga ccgccgcgag gcgttagcgg
    22381 gtcttgcgcg cgcactcttc gtccatcccg aaagcctcgg cgggcggccc ttctggggct
    22441 gttttcgacg cgcactcggc ccgcaagccg gcaagccgta gcgtgttata ctcgccgcgc
    22501 ctacggcccg aaagattgtt cgttgatgcc catgattaac ttgagtggat gcaaagtggg
    22561 actcaccgcg gaggcgcaga gggcgcagag actacgcaga ggcttgagcg attcactcga
    22621 cgttgggcgt aagtgttaag cctgcggcgc ttcctccgcg ccttctgcgc ctctgcggtg
    22681 aactgacttg cctgacactc tactaagggc acacccggcc gcctcgccgt tccgacttcg
    22741 cacatgagtt caaccgaagc aacctccgac tcgctcaccg gcacggtcaa ggggccgggc
    22801 gagcgcccgc acgagtacct gttcatcacg gccgaccaac tgcgcgcgcg catcggagag
    22861 ttcgtctact acgtcgcccg cgacggccgc gagcagcggc gcatcatcgg caacgtcacc
    22921 tcgcgccgcc tcgtgcgtaa cctccccgac tccttctccg ccgaccccga gacgccccct
    22981 gccgtcatct cctcgctcat cggcctcgac ggcgacggct gcgagcttta cgaaatcacc
    23041 gtcgagtcca tcggctactt cagcgagcgc ctcggcgact tcgtcaaccc gcgcatcccg
    23101 ccccggcccg gcgaccccgt cttcctcgcc tcctccgaaa cgctctccgg cgtgctctcg
    23161 tcgcggcgcg ccggagagcg cggctcgacc cacgtcggca gcctgctcac gcgcggcgcg
    23221 ggcgaggtgc ccatcgtcct ctcgacgcgc gacgtggtct ccacgcacct cgccatcctc
    23281 gcctccacgg gcgcgggcaa gtcctacacg gccggcgtca tcgtcgaaga gttgatgatg
    23341 ccctacaacc gcgccgccgt cctcgtcgtc gatccgcacg gcgagtacga cacgctgcgc
    23401 tccgtcgaag aggacgcgcg cttctccgac ccggccgacg gctaccgccc cgaggtcaaa
    23461 atctttacgc acgaccgcgt caaggtgcgc ttctcttctc tgaccgaggc cgacatcaag
    23521 tatctcctcc ccgaaggcac ctccgagaag atgctccatt atctctcgca ggcgttccgc
    23581 aaggtgacgg cccgcgcgcg cgagggccac gccggcggct cctacctctg gggctatcac
    23641 gatttgcgcg acgccgtcac cgagatgaag tatggcgact ctcgctcggg cggcgaggac
    23701 ggcatgggcg gctcttcgag caacgccccg accatcgaag ccctgctctg gcgtctcgac
    23761 aaccgcttcg accgccccaa ctcgctcttc tccgacagcg aacacatccc cttaaacgaa
    23821 ctcttccgcc ccggtcgctg cacggtgctc cagctctccg acatcgagca gaacgaacag
    23881 caagtggtcg tcgccacctt gctgcggcgc gtcaacaagg cgcgcgtggc gaccgtgcgc
    23941 gaggagtccg aagcgcgcac cgacacgcat ctgccctacc ccgtcttcac gctcatcgag
    24001 gaggcgcacc gcttcgcccc ggcgggccag acggtcgtct ccaccaacat tctcaagcag
    24061 attctctccg aggggcgcaa gttcggcgtc ggcatcgggc tgattacgca gcgacccggc
    24121 aagctcgatc aggacgtgct ctcgcagtgt atgacgcagg tcatcatgcg gatcgtcaac
    24181 cccgtggatc aggacacggt ggcgaagtcc gtggagggcg cgggccgcca actcatcgcg
    24241 gagctgccgg cgctcaccaa ggggcaggcc gtcatctccg gcgtcggcgt caacacgccc
    24301 gtcatgtgcc gcgtccgcga gcgcctcacg cgccacggcg gcgagacctt cgacgccccc
    24361 gacgagtggg cgcgctacca ctcgaaggcc gagcaacagc gacgcgagca ggacgacgcc
    24421 atcctcgtca gccccgcgcg cccgaagaag gccgacaagg ccgggccgtt ccagatttga
    24481 ggcggccagg gggcggcaaa gctattgaca cggccccccg cgcggcgtga gaatgtcgcc
    24541 caacagaggg gtcttacagg ggtcggcctg accgcctggc acttgaccgg gcgagaaggc
    24601 gccgccgccc catccaacca aaacttgtcc cgtaccttcc cgtccctata gtttttcttc
    24661 gagaggtaga gaacatgatt tcccgtccca gaccgttagc gccgctgctg ctgctgctcc
    24721 ttcttcccct cctcctgctc ctgacgctct ccgccatagg ccccgcgccc gcgcgcgcgc
    24781 agatgggcga cgccggcaag aacgaagacc cggtcttcaa ggagttcaag ggcgtgtcca
    24841 tcggcacgcc gatggacgaa gcgcgcaaga agctcggcag tccgaccgac aagggcgata
    24901 gccaggactt ctacgtcttc tccgacgacg agacggcgca agtcttctac gacagcggga
    24961 agaaggtgtc ggccgtcgcc gtcgtttaca cgggcggcaa gaacgtgccc accgctcagg
    25021 ccgtctttgg caaagaggtc gcgcccaagc ctgacggctc gcagttcctg cgcgtgctct
    25081 acccgaaggt caactactgg gtctcctaca gccgcacggc cggcgacacg cccgtcacct
    25141 ccatcatgat gcagcggaag tagacgggcg gcgcactcgg gcttctccca ccgccgcttc
    25201 cgccggcccg gccaaccctc ggccgggccg ggttgctttt ccgcgccgcg atgctaagat
    25261 aacccggcgg ttacaaattc cccttcagga cgacagtaaa cgaggctcgc ggcgcccccg
    25321 tgcgattgcc gtgcgcggag gcgtgcggca atcgcacggg gtgagttccg gggagatcgg
    25381 tggagaagag gatggccgaa gagagcacat cgacgagtcc ggagcagcgg cgcgtggccg
    25441 atctcatctc gtcgcgccgc gacgagatac tcaacctctg gatcacggag cggctggagt
    25501 ccgatgagtt ccgcgacgag ttgatctcga agaaggaact cctccaccag tcgcgtcagg
    25561 tgctggagat gctggcgcag gccctgcgcg aatcggacgg ctcggacttc gaccacccgg
    25621 cgtttgccga cctgcgcgcc tacctcaacg aaatctccca catgcgcgcc gtcaagggct
    25681 acacgccgac cgaaaacgcc acctacatcc tcgccctgcg aaacgtcgtc atgccgctcc
    25741 tcgtcgaaga gacggacacg aacaccgaca acctcgtgcg cgactcgaac tacttcacgc
    25801 ggctcttaga caagctgggc ctcgtgatgg tcgagaactt catccgctcg cgcgaggaga
    25861 tcatccgcca acagcgggcc gacatgatgg agctgtccac cccggtcatc aaggtctggg
    25921 acaagatact gacgctgccg atcatcggca cgctcgactc ccgccgcgcg cagatgatga
    25981 tggagtcgct gctgcaaaaa atcgtcgaaa ccggctcgac catcgccatc ctcgacatca
    26041 cgggcgtccg cacgatggac acgctcgtcg ccaaccacct catcaagacc gtgacggcgg
    26101 cccgcctgat gggcgcgcgc tgtatcctga ccggcgtctc cccggccatc gcgcagacga
    26161 tggtgcagct cggcatcgac ctgacgcaga tcaccacccg cgcgcagatg tccgacggca
    26221 tcaagctcgc gctcgaaatg atcgggcgca ccatcgtccc cacgaaggct ctggccaaga
    26281 tgcagagcaa cggcgccggc gccgacggcg tccccacgct caagttcgcc gagcagtcgg
    26341 cgaacaccac gcaagcggaa aacaagcatc gtgaatcgtg aagagctatt agcgattagc
    26401 tgttagctgt tagctaagaa ctaatcgcta atagctaaca gctaatcgct ctttaaggag
    26461 aacgaatgga cgggcagcgc atccccatct tgaaggtcgg ccgggtgttg atcgtgccga
    26521 ttcaggtgga catggacgat cagtcagtga tccacctcca ggagcgcatc ctctcggagt
    26581 tggagcgcac gggcgcccgc ggcgtcctca tcgacatctc gctccttgag atggtcgata
    26641 gcttcatcgg gcgcgtgctc tccgacatcg cggcgatggc gcgcattatg gacgcgcgca
    26701 cggtggtggt cgggatgcag ccggccgtcg ccatcacact cgtggagctg gggctggagt
    26761 tgcgcggggt ggacaccgtc ctcaacgtgg acgagggcat ccgcctgctg cggggcgagt
    26821 tcggcatcga cgacgacgag ggcgagacgg ccctcgggga gttctcggcc cttgcagagc
    26881 agatccatcg cccttgagga cgagcacgac atcgccgtgg cgcgcggcga ggtgcgcgtc
    26941 ctcgcggcgg ccctcggctt tcgtctcatc gaccagacgc gactcgccac cgtggcctcg
    27001 gagctggcgc gcaacgtcgt caagtacgcg cggcgcggcc ggatgatcgc gcagcccacc
    27061 gagagcacct cggggcgtca tggcctgcgt ctcatcttcg aggacaacgg ccccggcatc
    27121 cccgatcttc aggcggccat gcgcgacggc ttctccacgg gccgcagcct cggcaagggg
    27181 ctgcccggct cccgccgcct cgtcgatgag ttcgacatca agtcaacggt cggcagcggc
    27241 accatcgtca cggtcgtgcg ctggctctaa gaatcagagg tcagaggtta gaggttagag
    27301 gtcagtaaaa aacgggcttg ttcttaactg acctctaacc tctaacctct gacctcttct
    27361 ctgttgcttg tgagagtctt tcattcacag gagatcaagg acgaggcgca ggtcggtggg
    27421 gcgcgacgcg ccgtgcagcg cttcgccgcg ccgctcggct tctcggacga cgagctggcc
    27481 gagatcgcca tcgtcgtgca ggagatcggc accaacgcgg cgaagtactc gcgcgtcggc
    27541 ggccggctcc actggacgac gccgctcgac ggccccgccg gcctcgagct tttctactgg
    27601 gacaaggggc cgggcatctt cgacatcgaa gcggccgtgc gcgacggcgt aacgacgggc
    27661 gggggcctcg gcaccggctt cggcgccatc cgccgactga ccaacgagtt cgacgtttac
    27721 tccaccctgc gcgccaccac ctcgcgtctc ctccacgcgc gccgcaccac gcacggcacc
    27781 gtcgtcctct gccgcaagcg tgtcgccggc gcaaccgcgc cgcccccgga cgaaggcgag
    27841 ggccggcggc gcatcggcgc atggagtcgt tgccggacgg gtgaggacgt gaacggcgat
    27901 ggctacttca tcggccgcca cgacgggaat attttgctgg cggtcgttga cggtctgggc
    27961 cacgggcgcg gcgcgcgcga cgcgacggcc gccgccctcg gcgcctttga agactgggcg
    28021 ggcgagccgc ccgacgaggt gctcgcggcc gcgcacgacg cgctgcgctc cacgcgcggg
    28081 gccgtcgcgg cggccgtcgt catcgaccgg gaaggcgaga ccttccacta cgcgggcgtc
    28141 ggcaacatcg acgtgcgcgt cttcaattcc cccgagccgg tgcgccccgt ccccatcaac
    28201 ggcacgctcg gctcgcgcct ctccgctttg cgcgtctggc cccaccgctg gtcggagggg
    28261 acgaccatcg tgatggcctc ggacggcgtc agcgcgagct gggacatcgg ctcgtatccg
    28321 ggactgctgg ccaaaagccc tcaaatcttc gccggcgtcc tcctgcgcga ctacggccgc
    28381 gactccgacg acgcgaccgc actcgtcgtc agatgagttc aaggttcaaa gttcaaagtt
    28441 caaagggaag acacgtttcc gactttgaac tttgaacttt gaactttaaa ctctggactc
    28501 ttatggaagg catcacctcg acccggctcg gcgacatcta cgtgcgccgc gaggccgaca
    28561 tcatcaaggt gcgcgagcgc gtccgccggc tcgcgcgcga gttggggttc gactcgacga
    28621 cgcagatcaa gatcacgacg gccgtctccg aactcacccg caacatctac gagtacgccc
    28681 gcgccggcgc catcagttgc tccgtggccg agcgcggctc cgcgcagatc ggcctccaga
    28741 tcatcgcccg cgacaccggc cccggcatcg acgagaagca actccgggcc atcctccgcg
    28801 gcagctaccg ctcgccgtcg gggatgggcg tcggcctcgc cggcacgcgc cggctgatgg
    28861 acgagttcga gatcgagtcg aagccgcagg aggggacgcg cgtcctcgtc atcaagtggt
    28921 tggccgccgg cgcgggcgct ggcatcaagg cgcgcgtcgc ggagctgcgt gagttcttcg
    28981 cggccgaggt tgacgaggcg gcgatggagg agctgacgcg tcagaaccgc gacctcgtgc
    29041 aggtgctcaa cgaactcgaa gagaagcgcg aggcgctgga gcggctcaac agtcaactgg
    29101 gcgagagcaa ccgggagctg aacgaggcca acgccaagct gcgcgagctg gccgagatga
    29161 aggaggagtt cctcgccctc accacgcacg atctgcgctc gccgctcacg gtcatcagcg
    29221 gcgtcatcaa cttcttcacc tcgggccgcc tcggcgacat gacgcccgag cagaagaaca
    29281 tggtggcgat gatggagcgc aacacgcaca gcctcatcga actcgtcaac gacctgctcg
    29341 acgcctccaa gctcgaatcc ggctcgatgc gtctcgacct cacgtcggtc gatctgcgcg
    29401 cgctcacctc cgagctgcgc gacacgatgg agccgctggc gcgcgacaag gagatcgcgc
    29461 tcgaagccgg cttacccgac ggcctgccgc tcgtccgcgc cgaccgcgcg aagctccgcc
    29521 gcatcctcgt caacctcgtc tcgaacgccg tcaagttcac gccgaagggc gggcgcgtcg
    29581 agctgagcgc cgagccgcgg gacggcgcgg tgcgcgtctc ggtcgccgac accggcgtcg
    29641 gcatcgcgcc cgaagacatc cagcgcctct tcgacaagta cgagcaggcc cgctcgcgcg
    29701 ccacgcgcag cgagaagggc acgggcctcg gcctctacat cacgcgccag ctcgtcgaga
    29761 tgcacggcgg caagatcgaa gtccgctccg aggtcggcaa aggctcgacc ttctctttca
    29821 cgcttccgac ggagaacaag aagaagtgat aagtgataag tgataagtga tgagaaaaga
    29881 caaaagacga agtctttctc tcatcactta tcacttatca cttatcactc atcaccctta
    29941 tgaactacca ccttatcgga atctgcggga cggcgatggc ctcgctcgcc gggatgcttc
    30001 aggcgcgggg gcatcgcgtg accggctcgg acgagcacgt ctacccgccg atgtccacga
    30061 tgcttgaggg cttgggcatc cccgtcgcgg tcgggtatcg cgcggagaat cttgagccga
    30121 ctcccgactg tgtcgtcgtc ggcaacgcca taccgcgcgg caacgcggag ctggaggcag
    30181 tgctcaatcg acggcttctt tatcgctcgc aggccgaggt ggtcaaagag gagttcatcc
    30241 gcgggcggcg ctcgctggtc gtcgccggca cgcacggcaa gacgaccacg acgagcatcg
    30301 ccgcgtgggt tctggagcga ggcggtctcg acccgacttt cctcgtcggc ggcgtcgtgc
    30361 agaacttcgg cgtcagcttc cgcgtcacgg agagcgagta tttcatcatc gagggcgacg
    30421 agtacgacac ggcctacttc gacaaggggc cgaaattcat gcactacctg cccgagctgg
    30481 ccgtcgtcgg caacatcgag tttgaccacg cggacatcta taaagacttg gacgccgtca
    30541 agctcgcctt ccgccgcctg atgaacctcg tccccggcga gggccgcctc atcgccggct
    30601 gggacagccc cgatgtgcgc gccgtcgtcg ccgagatggg cgcgcgtctc cacacacggc
    30661 tcgaaactta cggcgcctgc gacgacgccc gctggcaggc gcgcgacatc cgcatcgacg
    30721 agcgcggcga gacgcacttc cgcgtcgtgc gcgacggcgc cgcgtgggcc gagtttcgca
    30781 cgccgatgat cggcgacttc aacgtccgca attgcctcgc cgtcgtcgtc gccgccgacg
    30841 cgtggggcgt ggagcgcgcg gccatcgccg atgcgctggc gactttcaag agcgtcaccc
    30901 gtcgcatgca ggtgcgcggc gagcgacgcg gcgtcaccgt catcgacgac ttcgcgcatc
    30961 acccgacggc cgtgcgcgag acgctgcgcg cgctccgtca aaggtacagg gatcgacggc
    31021 tcgtcgccgt cttcgagccg cgttcgtggt cttcgcgtct cgccgtcttc cagcaggact
    31081 acgtcgaagc tttgggtcag gccgactacg tcgtcatcgc cagcgtctac gaccagtcga
    31141 aggccgccga aaagggcagc gtgctcgaca cgcgagaact cgccgccgaa gtcggcgtcg
    31201 cgggcgattg cccgtcacac tgtatcgagg gcgccgacga aatcgtccgc cgcctcgccc
    31261 ccgaactccg cgcgggcgat gtcgtggccg tcatgtccaa cggcggcttt ggcggcatcc
    31321 acgaaaagct gctcgacgct ctcggcgcgg ggcgcgaggg cgacactcag gaggaagtcc
    31381 atcacgcatg aagactgtgc aactcatccc ccggcgcatc gccgccgtcg ccgccgtcgt
    31441 gttactcgcg ctgctgtgcg cccgtcttga tcctgcgttc gcgcaagacc tgcaaggcgg
    31501 cacgggcgtc ttcatcaagc gcgccttcgt caaagtcgcc gccaacgccc gcgccgcgcg
    31561 tcgtccacca cctcggcggg gcggggcgcg tccaaccacg cctccagcgc ccgaccgctc
    31621 cgcgctcgtc gatgaggcca tcaagatggg caacgccgag cgcgaggcgc agccgccgcg
    31681 cttgcaggac gccgagcgcg cctaccagct cggcgcgcgg ctcgacccgg cggacgcgcg
    31741 cccgcacgcg tggctcggcg atctctattt tcagcagcag cgattcacgg aggccgaggc
    31801 ggagttgcgt caggccgtcg cgctcgaccc gcgcgacgcc atctcctacg tccgcctctc
    31861 ctacatgttc agcaagctcg ggcgcttcga cgaggccgac gcggcggcgc ggcgcgtcca
    31921 gcagattctc cccaaagagt attacggcta ctgctcgatg ggctggagca agtttcggcg
    31981 caagagctac gacgaggccg cggccgccta tcgccgcgcc atccaactct ctccgcgcac
    32041 ctcgggcctg cacagcgacc tcgggttagt cctcctcagc cagagccgct acgccgaggc
    32101 ggccgacgcc ttccgtcacg ccatccagct cgacccgaac agcacgggcg cgctcatcaa
    32161 ctacggcgtc gtgctgcacc ggctcgggca gttgggcaag gcggccgaag cctacacgca
    32221 ggccgccgcg aaggccccgg ccgccacgca gccgcgctcg aacctcggcg ccatcagcta
    32281 cacgcgcgac gaccgcgcag cggcgaagtc tcactgggcg gagtccgtca aactcggcag
    32341 cgcctacgcg gttgaccgcg cgggcctgct catcctcgac ggcaagctcc ccgaggcgct
    32401 ggcgcagctc gaacagttca cgcaggccaa cgcggagaac gccgacggct ggctgctgct
    32461 cggcgacgtg cgccgcgccc gcggcgacgc cgaaggcgca cgcgccgcct acgcccgcgc
    32521 cgcgcaactc gcgcccgaat atgcgcgcct gccccgaccg aagctttaga gcaaaattcg
    32581 gtatcacgta ggacaggcat tcctgcctgt cttgagcgcg tgagcgcgaa taaactctcc
    32641 gcgctcactc gacgttcgga ctcgacgaac ctcatgttcg cgctcacgcg ctcaagacag
    32701 gcaggaatgc ctgtcctaca acaaacggaa aacctctctg gaagccctgt atgcttttgt
    32761 catgcgcgga gaacgattac aaacggcggg gtgaagacag gtcaagcccg aaggccgatt
    32821 agtccgtagt tttcgcactg ctgacgacct gcgccgggcg gtacggacat tgcctgtagt
    32881 taacccgtag gcgctttcaa ttacgagaac aacctaacta acatgatgac aagctactga
    32941 aggaggtaga actatgggac tgtgggacaa gatgttcggc cgcggcgtgg agaaggtgca
    33001 ggagcctgac gcgcagcagg ttttccagca actcaggcag aagtatcagg ccgctctgga
    33061 caagggcgat caggagcgca tccagtttca gaacctgcac gtcgagggcg gtaagctcta
    33121 cgtcaagggt ctggccccgt ccgaggaggc gcgcaccagc ttcatgaatc agatcgagat
    33181 gctcagggcc ggcgccgacg acgtggtcgc ggagatcact gtgcagcagt cgcagtcgca
    33241 ggccgccgcc gccggcgggg ccgccgtcag ctccccggcc gtgacccaca aggtcgagtc
    33301 gggcgacacg ctctcgaagc tcagcaagga gtattacggc accccggacg agtacatgcg
    33361 tatcttctac gcgaaccggg acaagctgcg cgacccgaat cgcatcaacg tcggacagga
    33421 tttggttatc cctcctgacg acaacggctg agtcgcgcta cacgccgcct cggaataact
    33481 acacccccgc gtttgaagca gcgtgattcg ttcacacggc ttcggacgcg ggggtttatt
    33541 gcgtttgcgt gcgaatcatc cgtattcact tccgcccggc gcatgtagcg gattgcattt
    33601 gagtgtgacc tccgcacgcc ctccctcacg gtcgggcttc cgccttgagg cacatccggg
    33661 cggaagcccg accgtgaggg agggcgcacg caatcgcaac tcgctaatgg aggactatgc
    33721 cgcgtatctg ccggccgcga cctcccgcgc ctgccccagc gagacgagcg tccgcaacag
    33781 ctcctcgacg cgctcgacgc gcgcgcgcgt gaactttcgc gccagctcct cgggcgtgac
    33841 gacgcctttc tgcgcggcga gcgtctggcg cagcgcgcgc gcctgttcgg gcagcgtctt
    33901 cggccacact tgtttcttct ccccggcttt ggcgggggcg gccggctcga agccctcgcc
    33961 gaacccggcc gcgacgccga ctgccggccg ctgaaaaccg ggccggagcc agcgcacgag
    34021 gccgcgacgc tcctcaccgg cgcgctcggc gttgagccgc acgaggcgtt cgagaatctc
    34081 ctcgtccgtc agggcggcgc tccagccgta ggcgtcggag acggcggcgt cgagccggtc
    34141 gtgaatctgc cggaggacgg agacgaggcc cgcctcgtgc gtgagccggt ctttatcagt
    34201 cagcgactcg ccggcgcgaa gtttctccag cacgttgtac atctcggtga cggtgaggcg
    34261 cgggtgctgc gcctgctggc gcttgcggtg cgcgtccaac gcctcgccga gttcgcggat
    34321 gcgcgcctgc tgctcgtcgg tggcgtcggg gaacgggaac ttctcaaagc aagccgtttt
    34381 tacgtaaaca ggatcattcc ctactcccaa cctactaccc gctgcgagtg cccacgtcac
    34441 atgaatgcgg ctcgacagca ctcccagaaa gtacgcatca ttaagagcga tattaaccaa
    34501 cttgttgtcg ggcagaactg atctgtccaa gagcacaaag aaccgatgct tggaagtctc
    34561 gacagtagcc acatatctat c
//
LOCUS       DASVES010000471         5488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_310059, whole genome shotgun sequence.
ACCESSION   DASVES010000471 DASVES010000000
VERSION     DASVES010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310059"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF240_19590"
     CDS             <1..231
                     /locus_tag="VF240_19590"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolytic cleavage of hydantoin with
                     aromatic side chains at the 5'position; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX7177471.1"
                     /translation="IAVGSDADILIFDPEREQTISARTHHMNVDYSAYEGRTIKGVVE
                     TVLSRGRVVIDGGEYRGKAGDGQFLKRGTCVS"
     gene            304..1023
                     /locus_tag="VF240_19595"
     CDS             304..1023
                     /locus_tag="VF240_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177472.1"
                     /translation="MTHRVKWYSLPLLALLLAATACNSGAPGANGGGAAPASGGNTAA
                     AGSSSAGAAAISADDKPHDVLMKAMRGQLDAKSYRVHVTTSMGGGPSNNIVIEYAAPD
                     RYRMVIEVPGGKQEFVIAGNAAYIKAPNGQWVRSPVGVADLIKQFRDPKMLEEMAKTT
                     DIKLVGPDTVDGTPTLVYQYTQNNVMGMNMKSTAKTWLAVADGLPRKTEAEGEYDGKP
                     TKTLMTITDYNSDIKIEAPVK"
     gene            1044..1769
                     /locus_tag="VF240_19600"
     CDS             1044..1769
                     /locus_tag="VF240_19600"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7177473.1"
                     /translation="MRHLLPRLFAAGALLLSASLPSRADDKVLPRLITVTGEAEVRVV
                     PDEVVFDLTVQTLDKNLRQAKAQTDERIKRLMELTRRYEVAPKDVQTDYIRVEPRYRG
                     NDETRLFAGYAVRKDLVFILRDVTKAEALLSEVIESGITRVNGVSFRTSQLRKHRDEA
                     RVLAIRAAREKAVALAGELGQKVGKAYSIEEEAPPSGRMMAQNVSSNAVGFADGDSSA
                     SSGTLALGQINVNARVTVRFELE"
     gene            1847..2179
                     /locus_tag="VF240_19605"
     CDS             1847..2179
                     /locus_tag="VF240_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177474.1"
                     /translation="MLFQNVISAKGPFPVKGSFNAESDDTMVIFVSGSVWSNTPGQWV
                     GVQILLDGNFIGDAVIFCNEATSHRAFIPVMIPATVAFGEHTITIQAAGSSTVADIND
                     FFNVYIMY"
     gene            2205..2333
                     /locus_tag="VF240_19610"
     CDS             2205..2333
                     /locus_tag="VF240_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177475.1"
                     /translation="MSHVANSEVIRHADGRVVVENGEFKGKSGDGQFLKRGTRVSM"
     gene            2424..2789
                     /locus_tag="VF240_19615"
     CDS             2424..2789
                     /locus_tag="VF240_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177476.1"
                     /translation="MSIPVPANKSINLSGLCLSPDAEQHVQFSCNGQTVEFSGIGNYG
                     PMQTQDGDILVAVHTGDATELTLSFWSSANPDPFLLGPEGPSDPPGIMYSVFSYDSQS
                     QEFISCSGNVMWNIFGLNG"
     gene            2855..4375
                     /locus_tag="VF240_19620"
     CDS             2855..4375
                     /locus_tag="VF240_19620"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX7177477.1"
                     /translation="MEATVDPEVIKHADGRVELREEALREVSRSPLYNEDLAPVPVAR
                     RNWTTYNYAALWISMAHCIPTYMLASGLISAGMNWWQALVTILLGNTIVLAPILLNSH
                     PGTKYGIPFPVFARASYGTIGSNLPALMRALVACGWFGIQAWIGGEALHTFFSALIPG
                     WPTLLGGAVGGHPPTMWLSFLLFWGLNIFIIYRGMDLLRHVENWAAPYVLVMTALLVA
                     WAIWRAGGLGYLLEQPGKFTTLAEFWPIFIPSLTAMIGFWATLSLNMPDFTRFGRSQR
                     EQVVGQVVALPTTMTVFAAMGVVITSAALVIYPDLKPADAWDPIKIISRFDQAWIVAV
                     SMFTVVVATLSVNIAANVVSPANDFANAFPRLVSFRTGGLITGIVGILMQPWRLLADP
                     SGYIFSWLLGYSGGLGSIAGVLVADYWLVRNKHLELGDLYRKQGAYTYTGGWNWRAVV
                     ATLLGCALAWIGLVVPALRPLYDYAWFVGFGVSAVTHLLLMKLSPPRVPEQAVVQD"
     gene            4501..5370
                     /locus_tag="VF240_19625"
     CDS             4501..5370
                     /locus_tag="VF240_19625"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7177478.1"
                     /translation="MPRIVKCGLIQATHAGPTDAPIEEIKRANTEKHLKMIAEAARQG
                     VQILCMQEVFTTPYFCAEQQTRWYEAVERIPDGPTVRLMQDVAREHSMVIVVPIYEEE
                     MTGVYYNSAAVIDADGKYLGKYRKNHIPHVAPGFWEKFYFKPGNLGYPTFETAYARVG
                     VYICYDRHFPEGARALGLGGAEIVFNPSATVAGLSEYLWKLEQPAHAVANGYFVGAIN
                     RVGHEQPWDIGEFYGQSYFCDPRGQIVAEAPRDADHLVVADLDFDKIREVRNTWQFFR
                     DRRPDTYEAIVAP"
BASE COUNT         1022 a         1868 c         1651 g          947 t
ORIGIN      
        1 atcgccgtcg gctccgacgc cgacatcctc atcttcgacc ccgagcgcga gcagaccatc
       61 agcgcccgca cgcaccacat gaacgtcgat tacagcgcct acgagggccg gacaatcaag
      121 ggcgtggtcg agacggtgct ctcgcgcgga cgcgtcgtca tcgacggcgg cgagtacagg
      181 ggcaaggcgg gcgacggtca gtttctaaag cgcggcacgt gcgtctcgta gtgacgcgcg
      241 ccggtcgctc agtttttatc tgtaactctt catcgacgct cggttgcgga aaggaatcaa
      301 cggatgactc acagggttaa atggtattcg ctcccactcc tcgccctcct gctcgcggcc
      361 accgcctgca attcgggcgc gccgggggcg aacggcggcg gcgccgcgcc cgcgtcgggc
      421 ggcaacaccg ccgctgccgg cagcagcagc gccggcgctg ctgccatctc ggccgatgac
      481 aagccgcacg acgtgttgat gaaggccatg cgcggtcaac tcgacgccaa atcgtatcgg
      541 gtacacgtca ccacctcgat gggcggcggc ccgtccaaca acatagtcat cgaatacgcc
      601 gcccccgacc gctatcgcat ggtcattgag gtgccgggcg gcaagcagga gttcgtcatc
      661 gccgggaatg cggcctacat caaggcgccg aacggccagt gggtgaggtc tccggtgggc
      721 gtggccgacc tcatcaagca gtttcgtgac ccgaagatgc tggaggagat ggcgaagacc
      781 acggacatca agctcgtcgg ccccgacacg gtggacggca ccccgacgct ggtctaccag
      841 tacacgcaga acaacgtgat gggcatgaac atgaagagca ccgccaagac gtggctcgcc
      901 gtcgccgacg ggctgccgcg caagaccgag gccgaaggtg agtacgacgg gaagccgacg
      961 aagacgctga tgaccatcac ggactacaac tcggacatta aaatcgaagc gcccgtcaaa
     1021 tgagacgcgc ggaggattag ccgatgagac acctacttcc ccggctgttc gcggccggcg
     1081 ccctgctcct gtccgcctcc cttccctcgc gggcggacga caaagtgctg ccgcgcctga
     1141 ttaccgtcac gggcgaggcc gaggtgcgcg tcgtccccga cgaggtcgtc ttcgacctca
     1201 cggtgcagac cctcgacaag aacctgcggc aggccaaggc gcagaccgac gagcgcatca
     1261 agcggctcat ggagctgacg cgccgctacg aggtggcgcc gaaggacgtg cagaccgact
     1321 acatccgcgt cgagccgcgc tatcgcggca acgacgagac acgcctcttc gccggctacg
     1381 ccgtccgcaa agacctcgtc ttcatcctgc gcgacgtgac gaaggccgag gcgctgctct
     1441 ccgaagtcat cgagtcaggc atcacgcgcg tcaacggcgt cagcttccgc acctcacagc
     1501 ttcgcaagca ccgcgacgag gcgcgcgtcc tcgccatccg cgccgcgcgc gagaaggccg
     1561 tcgccctcgc cggcgaactc ggccagaagg tcggcaaggc ctattccatc gaggaggagg
     1621 cgccgccgag cgggcgcatg atggcgcaga acgtcagctc caacgccgtc ggcttcgccg
     1681 acggagactc ttcggcctcg tcgggcacgc tcgccctcgg ccagatcaac gtcaacgcgc
     1741 gcgtcaccgt ccgcttcgag ctggagtgag cgccggctga aagccttcgc cagaagagac
     1801 acctcgacgc agtgaacccc tacagtctgc gaaaggagca tcaggaatgc tttttcagaa
     1861 tgtcatcagt gcgaaaggcc cgttccccgt caagggcagc ttcaacgccg agagcgacga
     1921 cacgatggtc attttcgtct ccggctcggt ctggtcgaac acgcccggcc agtgggtcgg
     1981 ggtgcagatt ctgctcgacg gcaacttcat cggcgacgcc gtgattttct gcaacgaggc
     2041 gacctcgcac cgcgccttca tccccgtaat gatcccggcg acggtcgcgt tcggggaaca
     2101 caccatcact atccaggcgg ccggcagctc caccgtcgcc gacattaacg acttcttcaa
     2161 cgtgtacatc atgtactagc cggcggcggc agaggtagat gaccgtgagt cacgtcgcaa
     2221 actcggaagt catccgccac gcggacgggc gcgtcgtcgt tgagaacggc gagttcaaag
     2281 gcaaatcagg agacggacag ttcctgaagc gcgggacgcg cgtcagtatg tgaccacggt
     2341 gccgggggcg accgccccgc tcccggcctc gtcctcaatc gcggggcgaa ctgacacaga
     2401 aaggaagaca catgccgacg tcaatgagca ttccagtgcc ggccaacaag agcatcaacc
     2461 tcagtgggtt gtgcctctcc cccgacgccg agcagcacgt ccagttctcc tgcaacgggc
     2521 agacggtcga gtttagcggg atcgggaatt atggcccgat gcagacacag gacggggaca
     2581 tcctcgtcgc cgttcacacc ggcgacgcca ccgagttgac gttaagcttc tggtcgagcg
     2641 ccaaccccga ccccttcttg ctcgggccgg aggggccgag cgaccccccc ggaatcatgt
     2701 acagtgtctt ctcgtatgac tcgcagagtc aggagttcat cagttgtagc gggaacgtca
     2761 tgtggaacat atttggactg aacggatgac gaccgaaagc gcgggacgtg tgttgccctc
     2821 tgaaggcccg acagaaacta tgaggtgatt gcctgtggag gcgacagttg accccgaagt
     2881 aatcaagcac gcggacgggc gcgttgagct gcgcgaggag gcgttgcggg aggtcagccg
     2941 cagccctctc tacaacgaag acctcgcgcc cgtccccgtc gcgcggcgca actggacgac
     3001 gtacaactac gccgcgctgt ggatcagcat ggcccactgc atcccgactt acatgctcgc
     3061 ctcgggcctc atcagcgcgg ggatgaactg gtggcaggcg ctcgtcacca tcctgctcgg
     3121 caacaccatc gtgcttgcgc cgattctttt gaactcgcac cccggcacca agtacggcat
     3181 ccccttcccc gtcttcgcgc gcgccagcta cggcaccatc ggctcgaacc tgccggccct
     3241 gatgcgcgcc ttagtggcct gcggctggtt cggcattcag gcgtggatcg gcggcgaggc
     3301 gctccacacc ttcttcagcg ccctcatccc cggttggccc acactcctcg gcggagccgt
     3361 cggcggtcac ccgccgacca tgtggctctc ctttcttctc ttctggggcc tgaacatctt
     3421 catcatctac cggggcatgg acttgctccg ccacgtcgag aactgggccg ctccttacgt
     3481 gctcgtgatg acggcgctct tagtcgcgtg ggccatctgg cgcgcgggcg gtctcggcta
     3541 ccttctggag caacccggca agtttacgac cctcgcggag ttctggccca tcttcattcc
     3601 ttccttaacc gccatgatcg gcttctgggc cacgctctct ttgaacatgc ccgacttcac
     3661 acgcttcgga cgcagccagc gcgagcaggt cgtcggacag gtggtcgcgc tccccacgac
     3721 gatgaccgtc ttcgccgcga tgggcgtcgt catcacctcg gccgcgctcg tcatttatcc
     3781 tgacctcaaa ccggccgacg cgtgggaccc cattaagatc atcagtcgct tcgatcaggc
     3841 gtggatcgtc gccgtctcga tgtttacggt cgtcgtggcc acgctgtcag tcaacatcgc
     3901 cgccaacgtc gtctcaccgg caaacgattt cgccaacgct ttcccgcgcc tcgtctcgtt
     3961 ccgcacgggc ggactcatca cgggcatcgt cggcatcctg atgcagccgt ggcgtctgct
     4021 cgccgacccc tcgggctaca tcttctcgtg gctcctcggc tactccggcg gtcttggctc
     4081 catcgccggc gtccttgtcg ccgactactg gctcgtccgt aataagcatc tcgaactcgg
     4141 cgacctctac cgcaagcagg gcgcgtacac ttacaccggc ggctggaact ggcgcgcggt
     4201 ggtggcgacg ctcctcggtt gcgcgctggc gtggatcggg cttgtggttc cggcgctccg
     4261 gccgctgtat gattacgcgt ggttcgtcgg cttcggcgtc tcggccgtca cgcatctact
     4321 gttgatgaag ctctcgccgc cgcgcgtccc cgagcaggcg gtcgtacagg attaaacgcg
     4381 gccctttagc aattatcagt tgagtgtaag gcaacgatga ctcaccgctg cgcccgcggc
     4441 ggtgagttga gatacattta cactcaagcg agagtatcaa gcgactgacc ggaggaactc
     4501 atgccacgga ttgttaaatg cggactcata caggccacgc acgccggccc gaccgacgcg
     4561 cccatcgaag agatcaagcg cgccaacacc gagaagcacc tcaagatgat cgccgaggcc
     4621 gcccgtcagg gcgtccagat tctttgcatg caggaggtct tcaccacgcc ctacttctgc
     4681 gccgagcagc agacgcgctg gtacgaggcc gtcgaacgca tccccgacgg cccgacggtg
     4741 cgtctcatgc aggacgtggc gcgcgagcac tcgatggtca tcgtcgtccc catctacgag
     4801 gaggagatga ccggcgtcta ctacaactcg gcggccgtca tcgatgccga cggcaagtac
     4861 ctcggcaagt accgcaagaa ccacatcccc cacgtcgccc ccggcttctg ggagaagttc
     4921 tacttcaagc ccggcaacct cggttacccg accttcgaga cggcctacgc gcgcgtcggc
     4981 gtctacatct gctacgaccg gcacttcccc gaaggcgcgc gcgccctggg cttgggcggc
     5041 gccgagatcg tcttcaaccc gtcggccacc gtcgcgggcc tctccgaata tttgtggaaa
     5101 ctggagcaac cggcccacgc cgtcgccaac ggctacttcg tcggcgccat caaccgcgtc
     5161 gggcacgaac agccgtggga catcggcgag ttctacggcc agagctactt ctgcgaccca
     5221 cgcggccaga tcgtcgccga agcgccccgc gacgcagacc acctcgtcgt cgccgacctc
     5281 gacttcgaca aaatccgcga agtccgcaac acctggcagt tcttccgcga ccgccggccc
     5341 gacacctacg aggcgatcgt cgcgccgtaa acgaggaagc cggtcggagc ggcgaaattc
     5401 ctctcgtgcg cggagcgtct tcgcctcgcg atagtcttgg ataagggttg cgacctgacc
     5461 cgcgccgact ttataaacgc gcctatgc
//
LOCUS       DASVES010000472        16859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_976988, whole genome shotgun sequence.
ACCESSION   DASVES010000472 DASVES010000000
VERSION     DASVES010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16859
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976988"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VF240_19630"
     CDS             <1..385
                     /locus_tag="VF240_19630"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7177479.1"
                     /translation="ATARDKGTAGRDTTFGYGIVQAKAALDYLSAPPAGPAAPSGLVV
                     TATGTNYIQLAWADNSSNETGFKIERCTGATCTNFAQIGTVGANVTSAKNSGLSRRTT
                     YRFRIRAYNAAGDSAYSNIANGTTR"
     gene            549..1592
                     /locus_tag="VF240_19635"
     CDS             549..1592
                     /locus_tag="VF240_19635"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7177480.1"
                     /translation="MLGRFIKAQERRHATRDTNRTVRPFEWGAEFVVEHLNGDDPHEV
                     VRRFAREAVLRSEEFYALPAIDDYALSDGLLTWTSAITTPSPENNTARARFFPAQPPK
                     RGRRQQEDGRARRAVVVLPQWNAQTHSHVDLCRMFARLGIAALRLSLPYHDDRRPAGL
                     ERADHLVSSNVGRTLQSMRQAVVDTRAAVRWLKSQGYERVGVAGTSIGSCTAFLAFTH
                     DEEIDCGVFNHVSSYVADVVWRGISTRHVREGFGDAVTIDEVREAWMPISPLAHAHRL
                     KDSRPRPMRFIAARHDLTFPADLSQQVIEEVRRHGVALDVQWLPCGHYTSGERPWVYL
                     DGWKIVTFIRRHL"
     gene            complement(1748..3493)
                     /locus_tag="VF240_19640"
     CDS             complement(1748..3493)
                     /locus_tag="VF240_19640"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177481.1"
                     /translation="MAHTPDSQNKEQEVFLQRLTHLAEALGTAREFVAIWRALRVFAE
                     AHVPCIGIFISLYDPERDARTAVYAYGDSQEPDVSTLPPMPVSAEGPNSRAVRTRQVV
                     ITNDYWNTKLQGKGQIGILVGPDNGLRPQSSLVVPMMTMGRVVGTVEVQSYEDDVYRP
                     EHVTAMRMAANLAAVAVENMRLLEREREARAAAEESNRLKDEFLATLSHELRTPLTAI
                     LGWAGMLRAGRLDEESATHAVEVIERNAHMQKQIVDDILDVSRIITGKLHIDAQPTDL
                     AAVARAAVEAMRPAADAKGLALEADLEAGDGVVLGDPARLQQVVWNLLANAVKFTPAG
                     GRVRVSVRAEAAHARLRVEDTGVGIRPDFLPFVFDRFRQGDQSTTRAFGGIGLGLSIV
                     RHLVELHGGAVRAESEGPDRGAAFTVELPLARHADLATRVEEGAPSETGRLATGEARR
                     AVLSGLRVLVVDDDEDTLALLATLLRRAGAEVATAADTRGALESFKAARPDVLLSDIG
                     MPGEDGYDLMREVREFERVSGGPATRAVALTAYVAPSDRDRALAAGFQRHLPKPVHPS
                     ELVEAIAGLSEPAQT"
     gene            complement(3716..4654)
                     /gene="ftcD"
                     /locus_tag="VF240_19645"
     CDS             complement(3716..4654)
                     /gene="ftcD"
                     /locus_tag="VF240_19645"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX7177482.1"
                     /translation="MVTKRVECVPNFSEGRRVETVERIAAAVESVEGALVLGTHVDAD
                     HNRSVITFVAPPETAVEAALRAVALAAELIDLREHAGQHPRMGATDVLPFIPLQGVTV
                     EECVGLAHEAGRRIWDELSIPVYFYESAALRSERANLADVRRGGFELLREQIATVEAR
                     APDVGERRLHESAGACVVGVRPLLIAYNVNLRTNDLGVARQIAARVRERGGGLRRLKA
                     LGFELASRGVVQVSMNLTSYAETNIHQAFEAVRREAEALGVEVLSSEVVGLLPQDALD
                     RAAAHFLRLENYSPDLVLENRIAAAIERKSKSGNSE"
     gene            5498..6136
                     /locus_tag="VF240_19650"
     CDS             5498..6136
                     /locus_tag="VF240_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177483.1"
                     /translation="MSGKNSSQLPFLLLLALAVVFGCKFANTKNTDSKTAGVNGSTPT
                     PTATPLSMVILDFDSKREELGKFTAPDRLDPASRVRGKVAIVEGEEGSYTLEGFDYSQ
                     FDEAELQAYGLTKEDLALKVEEIDTLVQTNCDKGRRISSYKTSDGKSVPAYALECETL
                     VFDYKAPAVIARKKFHSEEFVDSLQLSSATTDVTAIRPTEQVQKYVKGLRSR"
     gene            6218..6859
                     /locus_tag="VF240_19655"
     CDS             6218..6859
                     /locus_tag="VF240_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177484.1"
                     /translation="MSSGGYDNRPVADWARQNIRGRVEVRPLFSSSVGRIFTYTLLFC
                     VPGAFFALGFVALALGAKDPSTRYGPFVFGLLMLIPCGVIAMLGAYVRRGFAKSLDAE
                     GVNAALGKKFHWGKLYYVDHVTKHFRAGGVSRRIKDNQLELVFEGGKAVIPPLIRDRA
                     AVWALVNSMPAEVRDDGVPRAGRVPVAGGGPSGAQEELMAFLQALGPSKPGAQ"
     gene            complement(7130..8704)
                     /locus_tag="VF240_19660"
     CDS             complement(7130..8704)
                     /locus_tag="VF240_19660"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7177485.1"
                     /translation="MKLAAIDVGSNSIKLVVVDAASGDSFAVLARDKEPVRLGHGTLQ
                     EGHIGAEAIGRAAETIRRFRQRAEARGAEHVIAVATASVREAENADEFTDEMERATGL
                     RVEVLSGLEEARLIGIAAAQGCAPPGASLLNVDIGGGSTELSLVRGGVPAELYSVKLG
                     AVGLTDAYLANDPPKQKELRALREEVRAALERPARELKAARWQHATGTSGTILSLGEA
                     LRLKAERGADAEARGAQPPGAEITFNRLERLNERLAGMSTDERRTGAPGISAQRAEIV
                     VAGGQILEGVMRALKIDNLRSCDFALREGVIIDYLRRLEAESMPPVPDTSDPRLRGVH
                     AVGRRFGYEEAHAHQVARFAEKIFDRVAPAAGLTRQHRTLLSAAALLHDIGYHIAHES
                     HHKHTLYLIKNSELTGFSEAERGVIANVARYHRGSPPKDRHADFAALKPADRETARRL
                     AAILRLADALDRNHDGRVRDIKFKSDGRQARLSLRAHEDCERELEAVERRRDIFEQVF
                     DCELTVTQAAPAARPS"
     gene            9084..9632
                     /locus_tag="VF240_19665"
     CDS             9084..9632
                     /locus_tag="VF240_19665"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7177486.1"
                     /translation="MYAFMEIDWQGIFVPSNSVLEIIVRGTITYLALFTLMRVVLKRE
                     TGTVSLADLLMIVLIADASQNAMASEYKSVTEGVVLVMTLIFWNYALDWLGSRIPLFG
                     RLVHPAPLKLIEDGRLLRRNMRKEMITEDEMMSHLREQGVDEVSQVKAAYIEGDGRIS
                     VIKAEDGDSGASSARDDRGAVG"
     gene            9829..10038
                     /locus_tag="VF240_19670"
     CDS             9829..10038
                     /locus_tag="VF240_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177487.1"
                     /translation="MSVANELTSEVAMAMLKSDDRGERENLFGILLALHSTLRTLSAE
                     ERHRRRAKFLPEPPPAGRAASPLSH"
     gene            10117..12270
                     /gene="ppk1"
                     /locus_tag="VF240_19675"
     CDS             10117..12270
                     /gene="ppk1"
                     /locus_tag="VF240_19675"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX7177488.1"
                     /translation="MSTGAKQSASKGDATREAVAAVELPPSLLHHRHFLFNRELSWLE
                     FNRRVLEEALDPAHPLLERLKFLSIFSSNLDEFFMVRVSGLMEQLDEEVAELSPDGMT
                     VAEQLREIAARLKPMVEQQTRCLREDVLPKLAEAGILITPYRELSKEERQRLDEYFAE
                     KVFPVLTPQAVDSSHPFPYMSNLSLNLGLMVEPVEDKTGKFPQAMLGEPRFVRIKVPP
                     VVRRLVPVDDSGTRFTFLGELISANISSLFAGRRTGRAHLFRITRDADLDIREDEASD
                     LLSVMQEHLRRRRFGAAVRLEVTASMPEKLVRYLSKSLELSEEDVYRIDGPLNVPDLL
                     KLHQLDRPELKDAPLQVTVPAPLVKCKSVFDAVRQQDILLHHPFTSYVTVTDFIHQAA
                     HDPDVLAIKICLYRAGQDSPIVQSLMEASARGKQVAVLVELKARFDEENNIEWAQRLE
                     RAGVHVVYGLVGLKTHCKVALVVRREGNHLRRYVHLATGNYNPTTSRVYTDLGLLTAN
                     KKLGADATDLFNFLTGCSLQTKYRRLLVAPVNLRERMIEFIEREAAHRRKGRPARIIV
                     KINSLTDIQIVRALYAASQAGVHIDLIVRGTCTLRPGLAGVSETVRVRSVVGRFLEHS
                     RVFYFENGGEEEVYIGSADWMSRNLDRRVEVVVPVDDPNLKRLLRDEILDAYLRDNVN
                     ARLLQPDGSYIRARPADGEVPFSAQEFFQRQPATA"
     gene            complement(12489..12833)
                     /locus_tag="VF240_19680"
     CDS             complement(12489..12833)
                     /locus_tag="VF240_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177489.1"
                     /translation="MALIKKKTQKAIGKQVKKIVRKHGPEVALGLATSLVSSIIAAAE
                     GDKSAAGKSKKTKAKDAKAKDTKVKKAKAETGTKTKTKTKTKPKTKTSAKPKAKTDSQ
                     PAADAGKAADNG"
     gene            13051..14025
                     /locus_tag="VF240_19685"
     CDS             13051..14025
                     /locus_tag="VF240_19685"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX7177490.1"
                     /translation="MSSDRFVSERKGAWQRLEDLLGLLERTSLRRLEREEVRELGRIY
                     RRTASDLAIARAESRDPRLVGYLNSLVIRAHGRIYRADASEGGRRLWRFFAEDFPRTF
                     RRTWRYTALATAAFLLFAALAFFGTWIDPELSELGGIDPNSRELILNTRTHWWEQVNE
                     ANQLWSSVIMVNNIWVMLRAFALGALFGIGPLYEMARFGLQIGAVLSLTDRADFAADL
                     LTFMVGHGVLELSCVFITAGAGTLFGSALLLPGDLSRADALRLRGRDAVQLAAGCVPL
                     LVVAGVIEGFISPAALAPALKCGVGLLTGVALYAYLLLAGRETGTAAV"
     gene            14121..15047
                     /locus_tag="VF240_19690"
     CDS             14121..15047
                     /locus_tag="VF240_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177491.1"
                     /translation="MKVSRTKRLLRHLGYQFRRGMLPLKDSGFSSFDEEQILNRYIAE
                     LLPAEHSRTVVDIGAGDGRTGSNTLALFKSGWKGVGVEWDARKARKLARVYEQLPEAS
                     ACRLRVTPDNVVPLLEAYGVERNFGVLSVDIDSYDYWILDAVLARYRPQLVVTEINEK
                     IPPPIEFRVKYDPDFRSREHFYGYSIACLEELCALRGYALIDLEYNNAFLAPKELAGA
                     RALTPESAYRRGYLERPDRRAKFHRNQNMETLHAVGPEEGVRFIRQFFSQLAGKYELG
                     IATDAAAPEDAPVQDADYSTARPAESLQPAPR"
     gene            15156..16148
                     /locus_tag="VF240_19695"
     CDS             15156..16148
                     /locus_tag="VF240_19695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7177492.1"
                     /translation="MIFSGPQTSKLTTPLVSVVTATYNRSNVLRLAIESVRWQTFPDW
                     ELWVIGDACTDDTAEVVASFADPRIHFLNLPANVGEQSGPNNEGFRRTRGPFVAYLNH
                     DDLWLPDHLETALEGIEATGADLVFTLVDAVSASGKNSLWCVVPGMRYEPGLLVPASC
                     WVCRRALVEEVGAWRSYRECHSVPSQDWLYRAWRAGKDLRLIPRLTVVALPSGARRGA
                     YANRDVRENQHYFERIRREPDFRERELTAVVTNFDTPWLRWYPAPQLLLRALRNALVR
                     LCLAARLDPVAVRNFFIYFRKGAFLDRLRSRRGLEPLSRRRARSGNQSPRQENE"
     gene            16145..16717
                     /locus_tag="VF240_19700"
     CDS             16145..16717
                     /locus_tag="VF240_19700"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX7177493.1"
                     /translation="MKTTDAEEIDFRHEQGIIEGSAKDLRSITSDWTPVLELIDGVKL
                     REVKNVLKDNGFLTEVFRADWSLDAGGVDQVFQVVLAPGGLSAWHAHQFATDRLFVSH
                     GLVKVVLFDARTRSPTHGRLNEFRVGLLRPTLIVIPPGVWHGVQNISPESSCLLNMPD
                     RAYTYDDPDHWRLPPDTPEIPYSFAGPPTR"
BASE COUNT         2827 a         5657 c         5304 g         3071 t
ORIGIN      
        1 ggcgacggcg cgcgacaagg gcacggcggg tcgcgacacc accttcggct acggcatcgt
       61 gcaggcgaag gccgcgctcg actacctgag cgcgccaccg gcaggcccgg ccgcgccgtc
      121 cggcctcgtc gtcacggcga ccggcaccaa ctacatccag ctcgcttggg ccgacaattc
      181 gtcgaacgag accggcttca agatcgagcg gtgcacgggc gccacctgca ctaacttcgc
      241 gcagatcggc accgtagggg cgaacgtgac atcggccaag aactccggcc tctcgcgccg
      301 caccacctac cgcttccgca tccgcgccta caacgcagcc ggcgactcgg cctactcgaa
      361 catcgccaac ggaacgacaa ggtaagctca aggcaaaagt aaaaaggcaa aaggcaaaag
      421 tgggaaggca cgtcgcctct tcacttttgc cttttgcctt ttaccttttt acttttgcct
      481 tttcccgccc cgcgccttta ggatgactgt tccgccgaca ctacgagtct gaaaggccag
      541 ttgaacactt gctcggacgt ttcatcaaag cacaggagcg ccgtcacgcc acgcgcgaca
      601 cgaaccgcac ggtgcgcccc ttcgagtggg gcgcggagtt cgtcgtcgaa cacctgaacg
      661 gcgacgaccc tcacgaagtc gtccgccgct tcgcgcgcga ggccgtgctc cgcagcgaag
      721 agttctacgc gctgcccgcc atcgacgact acgcgctctc cgacggcctt ctcacctgga
      781 cgagcgcgat cacgacgccc tcgccggaga acaacacggc ccgcgcgcgt ttcttcccgg
      841 cgcagccgcc gaagcgcgga cgacgccagc aggaggacgg ccgagcacgc cgggccgtcg
      901 tcgtcctgcc gcagtggaac gcgcaaaccc acagtcatgt cgatctgtgc cggatgttcg
      961 cgcgcctcgg catcgcggcc ctgcgtctgt cgctgcccta ccacgacgac cggcgccccg
     1021 cgggtcttga gcgcgccgac catctggtca gctccaacgt cgggcgcacg ctccagtcga
     1081 tgcgtcaggc cgtcgtggac acgcgcgcgg ccgtgcgctg gctcaagagt cagggctacg
     1141 agcgcgtcgg cgtcgcgggc acgagcatcg ggtcatgcac ggcgttcctc gccttcacgc
     1201 acgacgaaga gattgactgc ggcgtcttca accacgtctc cagctacgtc gccgacgtgg
     1261 tctggcgcgg catctcgacc cggcacgtgc gcgagggttt cggcgacgcc gtgaccatcg
     1321 acgaggtgcg cgaggcgtgg atgccgatta gcccgctcgc ccacgcgcac cgcctgaagg
     1381 atagccggcc ccgccccatg cgcttcatcg ccgcgcgtca cgacctgacg ttccccgccg
     1441 atctctcgca acaggtcatc gaagaggtgc gccgccacgg cgtcgcgctc gacgtgcagt
     1501 ggctcccctg cggccactac accagcggcg agcgcccgtg ggtctacctc gacggctgga
     1561 agatcgtcac cttcatccgc cgacacctat aaaagcggtc agcaattagc ttttagctat
     1621 cagctatcag cccacggcat gcgccgtcgg ggcgattgac gcttacactc aaatcccttc
     1681 gttcgctctc taatagctga aagctaattg ctgaccgctg accgctccgt taacgattca
     1741 cgacttttca cgtctgtgcc ggctcggaca gcccggcgat ggcctcgacc aactcggagg
     1801 gatggactgg cttcggcagg tgacgctgga agccggcggc gagcgcgcgg tcgcggtcgg
     1861 acggggcaac gtaggccgtc agcgccacgg cgcgcgtcgc ggggccgccg ctcacgcgct
     1921 cgaactcgcg cacctcgcgc atgaggtcgt agccgtcctc gcccggcatc ccgatgtccg
     1981 agagcagcac gtcgggcctt gcggccttga aggattcgag cgcgccccga gtgtcggcgg
     2041 ccgtcgcgac ctcggccccc gcgcgcctca gtaaagtcgc cagcagtgcg agcgtgtcct
     2101 cgtcgtcatc gacgacgagc acgcgcaggc cgctcaagac tgcgcgtcgc gcttcgccgg
     2161 tcgcgagtcg tccagtctca ctcggcgccc cttcttcaac ccgcgttgcg agatccgcat
     2221 gccgcgcgag cggcagctcg acggtgaagg cggcgccacg gtcggggcct tccgattcgg
     2281 cgcgcaccgc gccgccgtgc agctcgacga ggtggcggac gatggagagt ccgagtccga
     2341 tgccgccgaa ggcgcgcgtc gttgactggt cgccctgccg gaagcggtcg aagacgaagg
     2401 gcagaaaatc gggccggatg ccgacgccgg tatcctccac gcgcaggcgc gcgtgggcgg
     2461 cctcggcgcg gacgctcacg cgcacgcgtc ctccggcggg cgtgaacttg acggcgttgg
     2521 ccagaagatt ccagacgacc tgctggagcc gggcggggtc gccgagcacg acgccgtcgc
     2581 ccgcttccag atcagcttcg agcgcgagcc ccttggcgtc ggccgccggg cgcatcgcct
     2641 cgacggccgc gcgcgccacc gccgcgaggt cggtcggttg cgcgtcgatg tgcagcttgc
     2701 ccgtgatgat gcgcgacacg tcgaggatgt cgtcaacgat ttgcttctgc atgtgcgcgt
     2761 tgcgctcgat gacctcgacg gcgtgcgtgg ccgactcctc gtcgagcctg ccggcgcgca
     2821 acatcccggc ccatccgagg atggccgtca gcggcgtgcg cagctcgtgt gagagcgtag
     2881 cgagaaactc gtccttgagc cggttcgact cctcggccgc cgcgcgcgcc tcccgctcgc
     2941 gctcaagcag acgcatgttc tcgacggcga ccgccgcgag gttcgccgcc atcctcatcg
     3001 ccgtcacgtg ctcggggcgg tagacgtcgt cctcgtatga ctgcacctcg acggtgccga
     3061 ccacgcgccc catcgtcatc atcgggacga ccagagacga ctgcgggcgc agcccgttgt
     3121 ccgggccgac gagaatgccg atctgcccct tgccttgcag cttcgtgttc cagtagtcgt
     3181 tcgtgataac gacctgtcgg gtgcgaaccg cgcggctgtt cggcccctcg gcgctgaccg
     3241 gcattggcgg cagggttgag acgtctggct cctggctgtc gccgtaggca tagacggccg
     3301 tgcgcgcgtc gcgctcgggg tcgtagagcg agatgaagat gccgatgcag gggacgtgcg
     3361 cctcggcgaa cacgcgcagc gcgcgccaga tggcgacgaa ctcgcgcgcc gtgccgagcg
     3421 cctccgccag atgcgtgaga cgttggagaa agacctcctg ctccttgttc tgactgtcgg
     3481 gagtgtgggc catcggtctg tttcggaaga gcggccgtcg tggccggcgc aaattatgcg
     3541 ccgccgccgc cagtttatcc agaactcccg cgccacctta gcgcctctcg cgtgagttca
     3601 tcggctcatc ggcgcattga gcacagtacc gcgagcggaa gcctcgcggg tctcgcgtgc
     3661 gccgggcggg actctcatgt aggccgcacc cgctcgcttc cgctcgcggt actgttcatt
     3721 cagagttgcc ggatttggac ttccgctcga tggcggcggc gatgcggttt tccagcacga
     3781 ggtcgggcga gtagttttcc agtcggagga agtgcgcggc ggcgcggtcg agcgcatcct
     3841 gaggcagcag gccgacgact tcgctggaga gcacttcgac ccccagcgcc tcggcctcgc
     3901 gacggacggc ctcgaacgcc tgatggatgt tcgtctccgc gtagctcgtc aggttcatcg
     3961 agacctgcac gacgccgcgc gaggccagct cgaagccgag cgccttgagc cgccgaagcc
     4021 cgccgccgcg ctcgcgcacg cgcgccgcga tttgtcgcgc cacgcccaaa tcgttcgtcc
     4081 gcaaattgac gttgtaggcg atcaggagcg ggcgcacgcc gacgacacac gcgcccgcgc
     4141 tctcgtgcag acgccgctct cccacgtccg gcgcgcgcgc ctcgaccgtc gcgatctgtt
     4201 cgcgtagcaa ctcgaagccg ccgcgccgca cgtcggccag attagcgcgc tcgctccgca
     4261 gcgccgcgct ctcgtagaag tagaccggga tgctcagctc gtcccagatg cgccggcccg
     4321 cctcatgcgc gaggccaaca cactcctcga ccgtcacccc ttgcagcgga atgaacggca
     4381 gcacgtcggt cgcgcccatg cgcgggtgct gtccggcgtg ctcgcgcaaa tctatgagtt
     4441 cggccgcgag cgcgacggcg cgcagggccg cctcgaccgc cgtctcgggc ggggcgacga
     4501 acgtgatgac ggagcggttg tggtcggcgt caacgtgcgt ccccagaacg agcgcgccct
     4561 cgaccgattc gacggccgcg gcgattcgtt cgactgtctc gacgcgccgc ccctcgctga
     4621 agttgggcac gcactccact cttttcgtca ccatgtcccc cgatagaaca atttcgattg
     4681 cgcgtcggat gtgccgggtg ttcgggagag aacacggatg aaacggatga gaacggatga
     4741 gaacggaatt gaatcaatcc gttctcatcc gtcccatccg tgttccttcg gcctcggccg
     4801 cgcgtcgccg ggctacgata ccacacgggc cgaacaccgc cgccgtcgtt cattaaagaa
     4861 aaaccgggcc gcgcgattgt cggattcaac cttcgcgcgg cccataccct gtctgttagt
     4921 ttttttctcg cccgggcgag atagcatgga caatctaaac ggggcgcgct aactcgccgt
     4981 taaggacaac ttaaatttcg gttaagatat ggtgtgatgt gaacgggcat gaatatcaaa
     5041 gagttacatg acgtgcgtcg tcatggattg cgcgaacgtt gcgcgaaccg ttcagaaaag
     5101 ctacagaacc ggaattgctg aaggaaaagg gcgctcccgc atggaagttg agttttcatg
     5161 gcagacgaaa taatccctat tggctttcgc aatagcttcc tcagccgtct gaagattgat
     5221 cggcggtatt ctggcgtcag gcgtgccgat aatcacgttg acagttccgc gtctccccga
     5281 ctactctctc ggcctgccgc cgcacgtcca cgccctatgc cgggaaagag tggtgcgcgc
     5341 cctgacccat tgcccttgct aaagcgctga gtaacacgcc ggacgagaga gccacgatca
     5401 ggggggccgg gttagtgttc tcgggttcta tcgccgcatc agcacgtatg cggcgtggca
     5461 ttgtaggcag acactacgtt ttgtcgaagg agattatatg tccggtaaaa acagttcaca
     5521 gttgccgttc ctgctgctgc tggcgttggc tgtcgtgttc ggctgtaagt ttgccaatac
     5581 gaaaaatact gactcgaaga cggcgggcgt gaacggctcc accccgaccc ccacggcgac
     5641 cccactttca atggttatcc tggattttga tagtaagagg gaggagctgg gtaaatttac
     5701 cgcgcccgac cggctcgacc ccgcgtcccg cgtgaggggc aaggtggcga ttgtcgaggg
     5761 cgaagaaggc agctacacgc tcgaaggttt cgactattcc cagtttgacg aggcggagct
     5821 ccaggcgtat gggctgacga aagaggacct cgccctcaag gtggaggaga tagacaccct
     5881 cgtgcaaacc aactgcgaca aggggagaag gattagcagc tataaaacgt cggacgggaa
     5941 gagcgttccg gcgtatgcac tcgagtgtga gactctggtc ttcgactaca aggctccggc
     6001 ggtcatcgcg cgcaagaaat tccacagcga agagttcgtc gatagtcttc aactctcttc
     6061 ggcaaccacc gacgtgaccg ccatcaggcc gaccgagcag gtacaaaagt atgtcaaggg
     6121 cctgcggagc agatgagcgc gacggcgctg gggaagggcg acgacggccg gttggagcca
     6181 gccggttggg tggccgtctc taaaggagat tattcggatg agctcgggcg gatacgacaa
     6241 ccggccggtc gccgattggg cgaggcaaaa cattagaggc cgcgtggagg tgaggccgct
     6301 ctttagttcg agcgtaggta gaatcttcac ctacacgctt ctgttctgcg tgccgggggc
     6361 cttcttcgcc ctcggctttg tcgccctcgc cctcggcgcg aaggacccgt ccacccgcta
     6421 cgggccgttc gtcttcgggc tcctcatgct gatcccctgc ggcgtcatcg ccatgctcgg
     6481 cgcatacgtg cgcagggggt tcgcgaagtc tctcgacgcc gagggcgtca acgccgcgct
     6541 ggggaagaaa ttccactggg ggaaactcta ctacgtggac cacgtcacga agcacttcag
     6601 ggcgggaggg gtgagccgcc gaatcaaaga caatcagctc gagctggtct tcgagggcgg
     6661 caaggccgtc atcccgcccc tgatccgcga ccgcgcggcg gtctgggctt tggtcaacag
     6721 tatgccggcc gaggtcaggg acgacggcgt gccgagggcg ggccgtgtgc ccgtggccgg
     6781 cggggggccg tccggggcgc aggaggaatt gatggcgttc cttcaagcgc tggggccgtc
     6841 caagcccggc gcgcaataat ggcatggcca ttaccagtgg ccgcgagatt acgacggtgt
     6901 taagtgctca cacgatcccc acgggacatg accgccatgg ggtcttttct cgtggcgaag
     6961 atgctgattt agaatcataa atgagtccgc gaatagctga gttctaaggc taagagcttt
     7021 tgctcgtcct tctcatcagg ccaaccaacc aacgatgttc gcgtggcgag gcggcccccc
     7081 tacccgaggc cattgacgcc cattcacagc accttttctt aaatttaggt taagaaggcc
     7141 gcgccgcggg ggccgcctgc gtgacggtca gctcgcagtc gaagacctgc tcgaaaatat
     7201 cgcggcggcg ttcgacggcc tccagctccc gctcgcaatc ctcatgggcg cggaggctca
     7261 ggcgcgcctg ccgcccgtcg cttttgaact tgatgtcgcg cacgcggccg tcgtggttgc
     7321 ggtcgagcgc gtcggcgagg cggaggatgg cggcgaggcg gcgagccgtc tcgcggtcgg
     7381 ccggcttgag cgcggcgaag tcggcgtgtc tgtccttggg cggcgagccg cgatggtagc
     7441 gcgcgacgtt ggcgatgacg ccgcgctcgg cctcggagaa gccggtcagc tcggagttct
     7501 tgatgaggta gagcgtgtgc ttgtggtgcg actcgtgcgc gatgtggtag ccgatgtcgt
     7561 gcaggagggc ggcggccgag agcagcgtgc ggtgctggcg cgtgaggccc gcggccggcg
     7621 ccacgcggtc gaaaatcttt tcggcgaagc gggccacctg atgcgcgtgc gcctcctcgt
     7681 agccgaagcg ccggccgacg gcgtggacgc cccgcagacg cgggtcggaa gtgtccggca
     7741 cgggcggcat cgactcggct tcgagccggc gcaggtagtc gatgatgacg ccctcgcgca
     7801 gggcgaagtc gcacgagcgc aggttgtcga tctttaaggc gcgcatcacg ccttcgagta
     7861 tctggccgcc ggcgacgacg atctccgcgc gttgcgcgct gatgccgggc gcacccgtgc
     7921 ggcgctcgtc cgtgctcatc ccggcaagcc gctcgttgag acgctccagc cgattgaagg
     7981 tgatctcggc tccgggcggt tgcgcgccgc gcgcctcggc gtcggcgccg cgctcggcct
     8041 tcaggcgcaa cgcctcgccg agcgagagga tggtgccgga ggttccggtg gcgtgctgcc
     8101 agcgcgcggc cttgagttcg cgcgcggggc gctccagcgc cgcacgcacc tcctcgcgca
     8161 gcgcccgcag ctccttctgc ttcggcgggt cgttcgccag ataggcgtcc gtcagcccga
     8221 cggcgccgag cttgaccgag tagagttcgg cgggcacgcc gccgcgcact aaagacaact
     8281 cggtggagcc gccgccgatg tccacgttca aaagcgacgc gccgggcggg gcgcacccct
     8341 gcgcggcggc gatgccgatg aggcgcgcct cctccagccc cgagagcact tcgactcgca
     8401 ggcccgtggc gcgctccatc tcgtccgtga actcgtcggc gttctcggcc tcacgcaccg
     8461 aggcggtggc gacggcgatg acgtgctcgg ccccgcgagc ttcggcgcgc tgccggaagc
     8521 ggcggatggt ttcggcggcg cgcccaatgg cctccgcgcc gatgtgtcct tcctgtagcg
     8581 tgccgtggcc gaggcgcacc ggctccttgt cgcgggccag cacggcgaac gagtcgccgg
     8641 aggcggcgtc aaccaccacc agcttgatcg agttcgagcc gacatcaatc gcggcgagtt
     8701 tcatttccgt cttcttcttc ccatctgcct tgtccgtcgc tgttattcta ccgcgcgttc
     8761 gttacatcga cgtaacggcg ggttaagata ttaacttaaa caccgaaatg tgagcttaaa
     8821 gtattgcgac gaacaaaact gtaggggagg gcaagtccgc ggcccttgct gtcggacaat
     8881 taacatggat ggacaggatg ggcaggatga agaacagatg atttgagatt tcagatttca
     8941 gatttcaaat cttctcctcc tgttcctcct gtccatccat gttaattcct ttcatgcgta
     9001 acgggcggcg gacttgccct tgcccccacg ggcgtgattg acgttgaggt tttgaaacag
     9061 acgctaattt caggaggggc cacgtgtacg cattcatgga aatcgactgg cagggcattt
     9121 tcgtcccgag caattccgtg cttgaaatca tcgtgcgcgg gacgatcact tatctcgctc
     9181 tgttcacgct catgcgcgtg gtgctcaagc gcgagaccgg caccgtgtcg ctggccgacc
     9241 tcctgatgat cgtgctcatc gccgacgcct cgcagaacgc catggccagc gagtacaagt
     9301 cagtgaccga gggcgtcgtc ctcgtgatga ccctcatctt ctggaactac gcgctcgact
     9361 ggctcggctc ccgcattccc ctcttcggcc gcctcgttca ccctgcgccg ctcaagctca
     9421 tcgaggatgg ccgcctgctt cgacgcaaca tgcgtaagga gatgattacc gaggacgaga
     9481 tgatgagtca cctgcgcgag cagggcgtcg atgaagtgtc gcaggtgaag gcggcctaca
     9541 tcgagggcga cggccgcatc agcgtcatca aggctgaaga cggcgactcc ggcgcgtcgt
     9601 ccgcacggga tgaccgcggg gccgtcggct gaatgccggc tgaagtaatc tttaaatctt
     9661 tgttacctcg cgcccggccg cgccgcacag ctttcacgcc tgaaagcacg cgtgctatgt
     9721 tgatttcggt tttcatgctc acacgcgccg ctgcggcccc tcgggcgcgg ctggagtggg
     9781 cgcttcaagg tctgatatct tcttcaacga agggacggcc cccccgtcat gtcagtcgcc
     9841 aacgagttga cgagcgaagt cgccatggcc atgctcaaga gcgatgaccg cggcgaaaga
     9901 gaaaatctgt tcgggattct gctcgcgctc cactccaccc tccgcactct ctccgcggag
     9961 gagcgccacc gacggcgcgc gaagtttctc ccggagcctc cccccgcggg ccgggccgct
    10021 tccccgctgt cgcactgaac ttttttcgcc gtcagccgtg aaccggcgcg cgccgcgacc
    10081 acacgtccac gcgccgggcg gcgccgactt tattcgatga gcacaggggc caaacaatcc
    10141 gcatcgaagg gcgatgcgac gcgcgaggcg gtagcggcgg tcgagctgcc gccgtcgctg
    10201 ttgcatcacc gccactttct cttcaaccgc gagttgagct ggctggagtt taaccgccgc
    10261 gtgctcgaag aggcgctcga ccccgcccac ccgctcctcg aacgcctgaa gttcctctcc
    10321 atcttctcct cgaacctcga cgagtttttc atggtgcgcg tctcgggcct catggagcag
    10381 ttggacgaag aggtggccga actctcgccc gacgggatga ccgtggccga gcaactgcgc
    10441 gagatcgccg cgcgcctcaa gccgatggtc gagcagcaga cgcgctgcct gcgcgaagac
    10501 gttctgccga agctggccga agccggcatc ctcatcactc cctacagaga actctcgaag
    10561 gaggagcggc agaggctcga cgagtacttc gccgaaaaag tcttccccgt actcaccccg
    10621 caggcggtcg attcttctca cccgttcccg tacatgtcga acctgagtct caacctcggc
    10681 ctgatggtcg agccggttga ggacaagacg gggaagtttc cgcaggcgat gttgggcgag
    10741 ccgcgcttcg tccgcatcaa ggtgccgccc gtcgtgcggc ggctcgtgcc ggtcgatgat
    10801 tcgggcacgc gcttcacctt cctcggcgag ctgatctcag ccaatatctc ctcgctcttc
    10861 gccggccggc gcacgggccg cgcgcatctc ttccgcatca cgcgcgacgc cgacctcgac
    10921 atccgcgagg atgaggccag cgacctgttg agcgtgatgc aggagcacct gcgccgccgg
    10981 cgtttcggcg cggccgtgcg tctcgaagta acggcgagca tgcccgagaa gctggtgcgc
    11041 tacctgagca aatcccttga gctttccgaa gaggacgtgt accgcatcga cggcccgctc
    11101 aatgtccccg acctcctcaa acttcaccag ctcgaccgcc ccgagctgaa ggacgcgccg
    11161 ctgcaagtca cagtccccgc gccgctcgtc aagtgcaagt cggtcttcga cgccgtccgg
    11221 cagcaggaca tcctcctgca tcacccgttc acgtcctacg tcaccgtcac cgacttcatc
    11281 catcaggccg cgcacgaccc cgatgtgctc gccatcaaaa tctgtctcta tcgcgcgggc
    11341 caggactcgc ccatcgtcca gtcgttgatg gaagcgagcg cgcgcggcaa gcaggtggcg
    11401 gtacttgtcg aactcaaggc gcgcttcgac gaggagaaca acatcgagtg ggcgcaaagg
    11461 cttgagcgcg ccggcgtcca cgtcgtctac gggctggtgg ggttgaagac gcactgcaag
    11521 gtggcgctcg tcgtccgccg cgagggcaac cacctccggc gctacgtcca cctcgccacc
    11581 ggcaactaca acccgacgac ctcgcgcgtc tacaccgacc tcggcctctt gaccgccaac
    11641 aagaagctcg gggccgacgc caccgacctc ttcaactttc tcacgggctg ctcgctccag
    11701 acgaagtacc ggcgtcttct ggtcgcgccc gtgaatttgc gcgagcgcat gatcgagttc
    11761 atcgagcgcg aggccgcgca tcgccgcaaa ggtcgccccg cgcgcatcat cgtcaagatc
    11821 aacagcctca ccgacattca gatcgtccgc gcgctctacg ccgcctcgca ggccggcgtc
    11881 cacatcgatt tgatcgtgcg cggcacgtgt acgctgcggc ccggcctcgc gggcgtctcc
    11941 gagacggtgc gcgtgcgcag cgtcgtcggc cgctttctgg aacacagccg cgtcttctac
    12001 ttcgagaacg gcggcgaaga ggaagtctac atcggcagcg ccgactggat gtcgcgcaac
    12061 ctggaccgcc gcgtcgaagt cgtcgtcccc gtggacgacc ccaacctcaa gcgcctgctc
    12121 cgcgacgaaa tcctcgacgc ctacctgcgc gacaacgtca acgcgcgcct gctccaaccg
    12181 gacggctcct acatacgcgc ccggcccgcc gacggcgaag tgccgttcag cgcgcaggag
    12241 tttttccagc gtcagccggc cacggcctga agcggcgctt gtcgcgaatc gtctccaaaa
    12301 cttaaagggc ggcgcgaaac catcgcgccg ccctttcgca atctttctct cgcaccgctt
    12361 ttagcgggtt gcatttgagg atgacctgcg aacgccctcc ctcacggtcg ggcttgcggc
    12421 cccggatgtg cctgaaggcg gaagcccgac cgtgagggag ggcgcacgca agcgcaactc
    12481 gctataaatc agccgttgtc tgcggccttg ccggcgtcgg ctgcgggctg gctgtcagtc
    12541 ttagccttag gcttagcgct ggtcttggtc ttaggcttgg tcttggtctt ggttttggtc
    12601 tttgtcccgg tttcagcctt cgctttcttc accttcgtgt ctttcgcctt cgcgtctttc
    12661 gctttcgtct ttttcgattt cccggccgcg ctcttgtcgc cctccgccgc cgcgatgatg
    12721 gaggagacga ggctcgtcgc cagacccagc gcgacctccg gcccgtgctt cctgactatc
    12781 ttcttcacct gtttgccaat cgccttctgc gtcttcttct tgatgagtgc catcggttcc
    12841 tctctttgcc gtctgagttg tccgccgcgg gcgcgcggag cgggctacgc taacacttat
    12901 tccgcggcgg agaaataggg gtgagcggaa tttaaccgaa acttcaggcg gcgtctatgc
    12961 ggcgaagttt ccgcgccatg cgctcgcctc ttacgacggc gcgcgcttta tgttgtggcg
    13021 cccgcggcct tatactccgc caccgagcct atgtcttccg accgtttcgt ctcagagcgc
    13081 aagggcgcgt ggcagcgcct cgaagatttg ctggggctgc tggagcgcac ctccctgcgg
    13141 cggctggagc gcgaagaggt gcgcgaactc gggcgcatct accggcgcac ggcctccgac
    13201 ctcgccatcg cccgcgccga gtcgcgcgac ccgcgactgg tgggctacct caacagtctc
    13261 gtcatccgcg cccacggacg catctatcgc gccgacgcct ccgaaggcgg acgccgcctc
    13321 tggcgcttct tcgccgagga ctttccgcgc acgttccggc gcacatggcg ctacacggcg
    13381 ctcgccacgg ccgccttcct cctcttcgcc gcgctcgcct tctttggcac atggatcgac
    13441 ccggagctgt ccgaactcgg cggcatcgac cccaactcgc gcgagctgat cttgaatacg
    13501 cgcacgcact ggtgggaaca ggtcaacgag gcgaatcaat tgtggagcag cgtcatcatg
    13561 gtgaacaaca tctgggtgat gctgcgggcc ttcgctttgg gggcgctctt cggcatcggg
    13621 ccgctctacg agatggcgcg cttcgggctc cagatcgggg ccgtcctctc gctcaccgac
    13681 cgcgcggact tcgccgccga cctcctgacc ttcatggtcg gccacggtgt tctggaactc
    13741 tcctgcgtct tcatcacggc cggcgccggc acgctcttcg gcagcgcact gctgttgccc
    13801 ggcgacctgt cgcgcgccga cgccctgcgc ctgcgcggcc gcgacgccgt gcagctcgcc
    13861 gccggctgcg tccccctgct cgtcgtcgcc ggcgtcatcg aaggtttcat ctcgccggcg
    13921 gcgctcgcgc ccgcgctcaa gtgcggcgtc ggcctcctga cgggcgtcgc gctctacgcc
    13981 tacctgctgc tcgccggccg ggagacgggg acggctgctg tgtgaagaaa gcgtctttcg
    14041 ggttcgaccg cccgcggagt cgaaggttat agtttcatca cgccgggcag cagtggccgc
    14101 gcggcgtgta aataaaactc atgaaagtca gtcgcaccaa acgtctgctg aggcacctcg
    14161 gctaccagtt ccggcggggg atgctgccgc tcaaggatag cggcttctcc agcttcgacg
    14221 aggagcagat actcaaccgc tacatcgccg agctgttgcc ggccgaacat tcgcgcaccg
    14281 tcgttgacat cggcgcggga gacggccgga caggctcgaa cacgctcgcc ctgttcaaaa
    14341 gcggttggaa gggcgtgggc gtggagtggg acgcgcggaa ggcgcgcaag ctcgccagag
    14401 tttacgagca gctgcccgag gcgtccgcct gtcgtctgcg cgtgaccccg gacaacgtcg
    14461 tcccgctgct ggaggcttac ggcgtcgaga gaaacttcgg cgtgctgagt gtcgatatcg
    14521 acagctacga ctactggatt ctggacgccg tcctcgcccg ctaccggccc cagctcgtcg
    14581 tcaccgagat caacgagaag ataccgccgc ccatcgagtt ccgtgtgaag tacgatcctg
    14641 actttcggtc gcgggagcat ttttacggct acagcatcgc gtgtctggaa gagctgtgcg
    14701 cgctccgcgg ctatgccctc atcgacctcg aatacaacaa cgcctttctc gcgccgaagg
    14761 agctggccgg ggcgcgcgcg ctgacgccgg agtcggcgta ccgccgggga tatctggagc
    14821 gccccgatag gcgggcgaag tttcaccgga accagaacat ggagacgctc cacgccgtcg
    14881 gccccgaaga gggcgtgagg ttcatccggc agttcttctc acaactggcg ggcaagtacg
    14941 agctgggcat cgccaccgac gccgctgcgc cggaagacgc ccccgtgcag gacgcggatt
    15001 actccaccgc ccggccggct gaaagcttgc aaccggcccc tcggtagcct tcgagcttgc
    15061 ccgcgggcgg ctgcggcggc gtgtggcgac gtgttagtat tctccgtccc catactcccc
    15121 tgtttcattt gtcttccgag tgctccctct tgccgatgat cttctctggc ccgcagactt
    15181 ccaagctgac cactccgctc gtctcggtcg tcaccgccac ttacaaccgg agcaacgtcc
    15241 tgcggctggc gattgagagc gtccgctggc agacctttcc cgactgggag ctgtgggtga
    15301 tcggcgacgc ctgcacggac gacacggcgg aggtcgtcgc ctccttcgcc gacccgcgta
    15361 ttcatttcct gaacctgccg gcgaacgtcg gggagcagtc cggccccaac aacgaaggct
    15421 tccggcgcac gcgcggcccg ttcgtcgctt acctcaacca cgacgacctc tggctgccgg
    15481 atcatctgga gacggccctc gaaggaatcg aagccacggg cgccgacctc gtcttcacgc
    15541 tcgtggacgc cgtatcagcc tcggggaaaa attccctctg gtgcgtcgtg cccggcatgc
    15601 gctacgagcc ggggctgctc gtccccgcgt cgtgctgggt gtgccggcgc gctctggtcg
    15661 aggaggtcgg agcgtggcgc tcttatcggg agtgtcacag cgtcccttct caggactggc
    15721 tgtatcgcgc gtggcgggcc ggcaaagacc tccggctgat tccgcgtctg acggtggtgg
    15781 ccctcccctc gggcgcgcgc cgcggcgctt acgccaaccg cgatgttcga gagaaccaac
    15841 actatttcga gcgcatccgc cgcgaacccg atttccggga gcgggagctg acggccgtcg
    15901 tcaccaactt cgatacccca tggctgcgtt ggtatccggc cccgcagctc cttctgcgcg
    15961 cgctccgcaa cgcgctcgtc cgactgtgcc tcgccgcgcg tctcgacccc gtggccgtga
    16021 gaaacttttt tatctacttt cgcaagggcg cgtttcttga tcgcctgaga agcagacggg
    16081 gactcgaacc cttgagccgg agacgcgctc gttccggcaa tcagtcgccc cgacaggaga
    16141 acgaatgaag acaacggacg ccgaggagat cgacttcaga cacgagcagg ggatcatcga
    16201 aggttccgcc aaagacctgc gcagcatcac ctccgactgg acgcccgtcc tggaattgat
    16261 cgacggggtg aaactgcgag aggttaaaaa cgtgctgaag gataacggct tcctgaccga
    16321 agtgttccgg gccgactggt cgctcgacgc gggcggggtc gatcaggtgt ttcaagtcgt
    16381 cctcgcgccc ggcggcctct ccgcctggca cgcacaccag ttcgccacgg atcgactctt
    16441 cgtcagccac ggcctcgtca aggtggttct cttcgacgcc cgcacccgct cgcccacgca
    16501 cggccgcctc aacgagtttc gcgtcggact gctgcgcccg accctcatcg tgattcctcc
    16561 cggcgtctgg cacggcgtcc agaacatctc accagagtcg agttgtctcc tcaacatgcc
    16621 cgaccgcgca tacacctatg acgaccccga ccactggcgt ctgccccccg acacgccgga
    16681 gattccctat tccttcgccg ggccgccgac gcgttagggc gccgcagacg cgctacgaca
    16741 gtccgatggg caactggaag aaagacgatg aaaagagtta tttggaaagc tcgccgacct
    16801 cgtcctgctc gacgctgacc cgcttgaaga tatacataac acccgcggga tttacggcg
//
LOCUS       DASVES010000473        10831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1059886, whole genome shotgun sequence.
ACCESSION   DASVES010000473 DASVES010000000
VERSION     DASVES010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059886"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..2897)
                     /locus_tag="VF240_19705"
     CDS             complement(63..2897)
                     /locus_tag="VF240_19705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7177494.1"
                     /translation="MRLAYFSPLSPQRSGISDYSEELLPHLAAGARIDLFVDGFEPSN
                     AETCARFRWFDYVSDPAALERLDDYDAVVYHLGNDHRYHSGIYDAASRRPGVVVFHDF
                     ALQDFFLGLARERGDVNIYLDEVGSEHGPEERARAAEALARGGLPSHFAAPLHFPLNR
                     RLALGAEAVITHSRLSAARFEQLAPGTPVAHVNHHVTEGAASREPPTRDTADAANARP
                     VRLASFGLITPGKGVGHALRALSALKDDYDFRYTLVGETNPFYDVRALIREHGLSERV
                     EITGHVTLDEFERRIAETDVALNLRTRTCGEASGSLCRIMAAGVAAVVSDAGWFSELP
                     EGAVVKVDAGRWLDAELRAYLERLLADAALRESVGARARRHVLAEHRIEQSAARYLSF
                     IDEVIGGRARRRLVRGVSAEISRLRLDPEADTSVISAVAAEVAALAPERAFAAAATTR
                     HAPEADTHAASSADTSAAASLATSTSEESASASDERDAGSASSSSEQATTAVADEGAA
                     TTHAPGRLRKVEGVDYKRAAVEYPRKLDAERHHYLFTKPFYNLSNKPPKHEGDGMDPE
                     THRHFCDFANLAVALALPPGRRLLDVGCGSGWLSEYFARLGYEVTGIDISPELIEIAR
                     QRTACVPYDVDHETPIVCRFLVHDAESAPLDEVFDAAFCYDSLHHFEDERAVMRHLSE
                     MVSYGGMLFILEGGKPDEGSETAAELTDVMRLYETLESPFDPAYLRALLREHGFLVVG
                     DYVSVNGLFERDSVENERVRFVQQDLNYLLCKKVTHGVSPADGVPDSRAPGRLSARIT
                     AAAATATDDDAMPGNDAVPGDEAPARPPTLSPGADFGLSLEIENTGDTVWLTGAADRR
                     GAVMLGVRVFDDEGRVVLFERHGEPPLPRPLAPGERVRLALGFRAPDAPGRYQLKLDL
                     VAQHVCWFEQHGSAPLVMAFEVQGIDSSAH"
     gene            complement(3009..3170)
                     /locus_tag="VF240_19710"
     CDS             complement(3009..3170)
                     /locus_tag="VF240_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177495.1"
                     /translation="MNSEASAKTDESAGAASAWQSLRSSPAVRSALYAYVLTRLAVFV
                     VLIVGGHES"
     gene            complement(3359..3925)
                     /locus_tag="VF240_19715"
     CDS             complement(3359..3925)
                     /locus_tag="VF240_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177496.1"
                     /translation="MIRTRRRITAALALACALLLCASALSGCGGRGVENVAQSGGQPA
                     AGAAAGTTAPTAPGPLPDEGYRAQITLLDAPARMRAGQKQTINVNVRNTSNVTWIAPH
                     DQEGNKYVIAVMNSWLQPDGTLATNMDGRYGVQRDLAPGEEAELPLVITAPKNPGDYI
                     LELDMVQEQVTFFKDKGSQPLRVNVKVE"
     gene            complement(3975..6236)
                     /locus_tag="VF240_19720"
     CDS             complement(3975..6236)
                     /locus_tag="VF240_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177497.1"
                     /translation="MSEAPVIARAALPRRARDFLRRVGLGDALMLLYLAAFAREYLWA
                     VADQRLAWALTLLLAALAWYFYVATGEERERTPPAFWLVVALPLAFVYALRVPFPDVS
                     FDVLNYRLLHAERALAGPLFMPGDFFPAPIPFNPSPDIVTGLCRRLLGYRLGTLPNLL
                     ALVWAAQVVERLLRPYISGARRRAFGVLLIMLCEHSLFEINNYMVDVLALPLILEAVY
                     VALRRDGERGSVRVAAARLGLLLGVAVAFKLTNLTVACFIALIYTYQLLFKLRPARRE
                     ILLSLALAAAAFLVPLLPFTFYIYGETGSPVFPLFNTIFESPYWAAQDWRDPRWGPAA
                     AGWWGKLLWPVASVFTPERLSELNVYSGRLALGFVAALAGLVLARRDALVRALCLVVL
                     LDSLTWAATTGYVRYVLHLELLGGALVLMLAASAARANVRMRAALKYSLACALWLALA
                     AQGALACRYVLRTEWGGRPTVFSQPRTHLREAAHLLLRDHSLADFLPDKERALFAGVE
                     VWVDSSMKSNGFEALLRPRAPVINARQPEYFTTRASEQRFAEALDRAAGKRLFSLSFP
                     EDLKSARASLERRGLKIVGQTPVTIPFYSPHNNIGLYLLEVERGAAVPSPAPDGRAAA
                     LPDGAYRARLSLPRAPARMKPGERASLLVRVRNEGSAPWPARGNAQGMLQVNVGDRWL
                     ATDGRTVVNDLDGRTALPRDLGSGDEAELTLNVIAPKAPGEYVLEIDMVHEGVTWFYE
                     KGSQTLRVSVRVE"
     gene            6436..9126
                     /locus_tag="VF240_19725"
     CDS             6436..9126
                     /locus_tag="VF240_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177498.1"
                     /translation="METPTVQAAPANVKTGRGAWKTLLGRLAGAPRAREALVILGFFA
                     VTALMTWPWVTRMRDAVGDVGDPYMIAWTLWWDYHQTFNDPLNLFHANVFHPYRYTLA
                     FSENDYGIALLFFPLYALGARPLTVHSVATFCGFVFCGYAAFRLARTLTGSVAAGWVA
                     GVVFAFVPYRFHLLSHLHYLFAGWIPLLLEALVLFARERSWKRAAWLGVAFLMNALTC
                     ITWFILTLPALALSAAFLVARYRLARERAFWVRGAAALGLASLALLPFLLPYYHVSRM
                     YNFAWGREEVVKQSPRVSTWLSAETRNRVWRWLGARSDGSPLFPGLLPLLLALAAFTR
                     LGARRGDTAAALEGVAVEKDSATARRAEWWTELLDAAALAAGVLALVAAVFEGTQGWG
                     GTVFGVFTSDRALFVLACALVARLCVAYPLWFRPSGNLIETMRAGRHSDAVWLGALWA
                     AVGFLMSLGTNSLFYRLVYDHLFLFRSQRLPARGAMLAYVGLAVLAGLGAVRVAGWVA
                     RRRRDVSAGAVCAVLILALLFELRAAPLRFVRGAVFPDEVTLRLKETPMRGGVVELPA
                     GPDGHNHLYMLRAADHARPLVNATSSFVPQPIWELHELTRGGPIAPRLLDLLEELSTS
                     YVVVHNARLAPERHTDYEQFFARAVATGRLRFVRRFEGRDDLYAVTMFEPEARGEEPL
                     PFVSTMRRLDEMLAEDPVNLLGEFFEWGQTLERLHKASFGRPPRYEEFMPDAQRLAAG
                     VLAGDEDGRTQLENNYRALISDWTRRAAFVSSFGALSDEQFIDALARNAGLSPPADER
                     AALAEALRSGTETRAGVLASLVARREFVERENYPSTVLLHYFGYLRRDPGAPPERDAS
                     GFDFWITELEHAGGDRARLGRAFAGSGEYLELRKRLGKQP"
     gene            complement(9133..10650)
                     /locus_tag="VF240_19730"
     CDS             complement(9133..10650)
                     /locus_tag="VF240_19730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7177499.1"
                     /translation="MSEEMEGNSARRRRPRGGALAWAALAGLGLVLYLHSVPENPPGF
                     FADESSIAYNAHLVARHGADEHGARFPLFFRAFGEYKSPVYVYLLAALFKLTGPSIAV
                     ARSLSAVLGAAAALLLGLVAARSVSVRPRAVGLTVAVSALLTPWLFELSRLVFEVALM
                     PLVLALLLLALRSAHERGRWTFGDNVRVALALALITYTYSVGRLLAPLLALGLLLFAG
                     RGRWWSVAFRTWLLYAVTLLPLLVFFVEQPGALGERFAQVSFIKPGMSWMEVALRFCQ
                     NYAGHFSPRGWLVAGDPEPRHHVQTMGSLLAPTVVAAAAGLVLLLRRRGADAWWRFVV
                     YGLLVSALPSALTLDHFHTLRLVGVPVFLLLLTAPALERLLTPGAHTRARRVVFAALL
                     LTTLLQGALFQWHFRQAAPARWHSFDAHYPEVFAAAMSRPERPVHLIDNNGAPGYIHA
                     YWYATLAGTDPARAFLRLPKEARPPAGALVISTELPCHNCRIISERAAFRAYIAE"
BASE COUNT         1694 a         3562 c         3900 g         1675 t
ORIGIN      
        1 gtcacccgct cgctaccgct cgcggtactg tgcttgccga caccttccat tgaatcaatg
       61 attcaatgag ccgatgaatc aattccttga acctcgaacg ccatgacgag cggcgcggag
      121 ccgtgctgct cgaaccagca gacgtgctgc gcgacgaggt cgagcttgag ttggtagcgg
      181 ccgggcgcgt cgggggcgcg gaagcccagc gcgagacgca cgcgctcgcc cggcgcgagg
      241 gggcgcggga gcggcggctc gccgtgacgc tcgaagagga cgacgcggcc ctcgtcgtcg
      301 aagacgcgca cgccgagcat cactgctccg cgccggtcgg ccgcgcccgt gagccagacg
      361 gtgtcgcccg tgttctcgat ttcgagcgac aggccgaagt ccgcgccggg tgaaagcgtc
      421 ggaggccgtg ccggtgcttc gtcgcccggc acggcgtcgt tgcccggcat ggcgtcatcg
      481 tccgtcgccg tggcggcggc ggcggtgatg cgcgcggaga ggcggcccgg agcgcggctg
      541 tcggggacgc cgtcggctgg cgagacgccg tgcgtgacct tcttgcagag caggtagttc
      601 aagtcttgct ggacgaagcg cacgcgctca ttctcaacgg aatcgcgctc gaagaggccg
      661 ttgacgctca cgtagtcgcc gacgacgagg aagccgtgct cgcggagaag cgcgcgcagg
      721 tacgcggggt cgaatggcga ctccagcgtc tcgtagaggc gcatcacgtc cgtgagttcg
      781 gccgcggtct cggagccttc gtcgggcttg ccgccttcga ggatgaagag catcccgccg
      841 taagagacca tctcgctcag gtggcgcatc acggcgcgct cgtcctcgaa gtggtggagc
      901 gagtcgtagc agaaggcggc gtcgaagact tcgtcgagcg gggccgactc ggcgtcgtgg
      961 acgaggaagc ggcagacgat gggcgtctcg tgatccacgt cgtaggggac gcaggccgtg
     1021 cgctggcggg cgatctcgat gagttcgggg ctgatgtcga tgccggtgac ttcgtagccg
     1081 aggcgcgcga aatactcgga gagccagccg gagccgcagc ccacgtcgag gaggcgtcgg
     1141 cccggaggga gcgcgagcgc gacggcgagg ttggcgaagt cgcagaagtg tcgatgcgtc
     1201 tcggggtcca tcccgtcgcc ctcgtgcttg ggcggcttgt tcgagaggtt gtagaagggc
     1261 ttggtgaaga gatagtggtg acgctcggcg tcgagcttcc gcggatactc gacggccgcg
     1321 cgcttgtagt caacgccctc gaccttccgc aaacgccccg gcgcgtgcgt cgtcgccgcg
     1381 ccttcgtccg cgaccgctgt cgtcgcctgc tccgacgagg acgacgctga gccagcatcc
     1441 ctctcatcgc tcgcgcttgc agactcttca gaagtagaag tagcgagaga ggcagcggcg
     1501 gacgtgtcgg cggaagaagc ggcgtgcgtg tcggcttcgg gcgcgtgacg ggtcgttgcg
     1561 gcggcggcga aggcgcgctc gggcgcgagg gcggcgacct cggcggcgac cgcgctgatg
     1621 actgacgtgt cggcctcggg gtcgagacgc aggcgcgaga tttccgcgga gacgccgcgg
     1681 acgagacggc ggcgggcgcg gccaccgatc acttcgtcga tgaaagagag atagcgcgcg
     1741 gcgctctgct cgatgcggtg ctcggcgagg acgtgtcggc gcgcgcgcgc cccgacgctc
     1801 tcgcgcaagg cggcgtccgc cagcaggcgt tcgaggtagg cgcgtagctc cgcgtcgagc
     1861 cagcggcccg catccacctt gacgaccgcg ccctccggca gctccgagaa ccagccggcg
     1921 tcggagacga cggccgcgac gcccgcggcc atgatgcggc acagactgcc cgacgcctcg
     1981 ccgcacgtgc gcgtccgcag gttgagcgcc acgtccgtct ccgcgatgcg ccgttcgaac
     2041 tcgtcaagcg tgacgtggcc cgtgatctcg acgcgctcac tcagcccatg ctcgcggatg
     2101 agcgcgcgca cgtcgtagaa cggattggtc tcgccgacga gtgtgtagcg aaagtcgtaa
     2161 tcatccttga gcgcggaaag cgcgcgcagg gcgtggccga cgcccttgcc gggcgtgatg
     2221 aggccgaagg aggcgaggcg caccggacga gcgtttgcag cgtcggccgt gtcgcgcgtg
     2281 ggcggctcgc gactcgcggc cccctcggtg acgtggtggt tgacgtgggc gaccggggtg
     2341 cccggcgcca actgctcgaa gcgcgcggcc gacaggcgcg agtgcgtgat gacggcctcg
     2401 gcgccgaggg cgaggcggcg gttgagcggg aagtgaagcg gcgcggcgaa gtgtgacggc
     2461 aatccgccgc gcgcgagcgc ctcggcggcg cgcgccctct cttcggggcc gtgctcggag
     2521 ccgacttcgt cgaggtagat attcacgtcg ccgcgctcgc gggcgagacc gaggaagaag
     2581 tcctgcaagg cgaagtcgtg gaagacgacg acgccggggc ggcgcgaggc cgcgtcgtaa
     2641 atcccggagt gatagcggtg gtcgttgccg aggtgataga cgacggcgtc gtagtcgtcg
     2701 aggcgttcga gcgcggcggg gtcgctgacg tagtcgaacc agcggaagcg cgcgcacgtc
     2761 tcggcgttcg acggctcgaa gccatcgacg aagagatcga tacgagcgcc ggcggcgagg
     2821 tgcggcagca actcctcgct gtagtcggag atgcccgagc gctgcgggct taaggggctg
     2881 aagtaagcga gtctcatcaa gttcgtcaat cgtaaatcgt gaatcgtaaa tcgtaaagcc
     2941 cgccggccgc ccgtcggact tcatcgctca aggcgccacc gtctcaacca cgatttacga
     3001 tttaccattt acgattcatg gccgccgacg atgaggacga cgaagacggc gagccgcgtc
     3061 agcacgtagg cgtagagcgc cgagcgcacg gcgggcgagc tgcggaggct ctgccacgct
     3121 gacgccgcgc cagcgctttc atccgtcttc gctgacgctt cgctattcat cacaggccct
     3181 tctctgggag cacgggcgcc ttgcagcccg ccgccggcgc cgacaggcac agtaccgcga
     3241 gcggtagcga gcgggtctcg cgtgcttgag acgagccttt agcgtagacg caacccgctc
     3301 gctaccgctc gcggtactgt cccacgctca cggcacgtta atgtgcgcgt gctcccggtc
     3361 attcaacttt gacgttgacg cgcagcggct gcgacccctt gtctttgaag aaggtgacct
     3421 gctcctgcac catgtcgagt tcgaggatgt agtcgccggg gttcttcggc gcggtgatga
     3481 cgagaggcag ctcggcctcc tcgccggggg cgaggtcgcg ctgcacgccg tagcgcccgt
     3541 ccatgttcgt cgccagcgtg ccgtcgggtt ggagccaact gttcatgacg gctatgacgt
     3601 acttgttacc ctcctgatcg tgaggggcga tccacgtgac gttgctcgta ttcctgacgt
     3661 tgacgttgat ggtctgcttc tgcccggccc gcatccgcgc gggcgcgtcg agaagcgtga
     3721 tctgcgcccg gtagccctca tcgggtagcg gccccggcgc cgtgggcgct gtcgtcccgg
     3781 cggccgcgcc cgcggccggc tgcccgcctg actgggcgac gttctccacg ccccggccgc
     3841 cgcagcccga caacgccgac gcgcacagta gtagcgcaca cgccagagcg agcgcggccg
     3901 tgattcttct cctcgtccgt atcatttcgg aaacgtccct tcttttattc gatctcgtcg
     3961 aaacggtgca atggtcactc cacgcgcacg ctcacgcgca gggtttgtga tcccttctca
     4021 taaaaccacg tcacgccctc gtgtaccatg tcgatctcca gcacgtactc gcccggggcc
     4081 tttggcgcga tgacgttgag cgtgagttcc gcctcgtcgc cggagccgag gtcgcgcggc
     4141 agcgcagtcc ggccgtcgag gtcatttaca acagtccggc cgtcggtggc aagccagcgg
     4201 tcgccgacgt ttacctgaag catgccctgc gcgttgccgc gcgccggcca cggggctgac
     4261 ccttcgtttc tgactctgac gagcagcgac gcacgctcgc ccggcttcat tcgcgcgggc
     4321 gcacggggca gggagaggcg cgcgcggtac gcgccgtcgg ggagcgcggc ggcgcgtccg
     4381 tccggcgcgg gcgaggggac ggccgcgccc cgttccactt cgagcaggta gaggccgatg
     4441 ttgttgtgcg gcgagtagaa gggaatcgtc acgggcgtct ggccgacgat cttgaggccg
     4501 cgtctttcga ggctcgcgcg ggccgacttc aggtcttcgg ggaaggagag cgaaaagagt
     4561 cgcttgccgg cggcgcggtc gagcgcttcg gcgaatcttt gttcggacgc gcgcgtcgtg
     4621 aaatattcgg gttgtcgcgc gttgatgacc ggcgcgcgcg gacgcagcag cgcctcgaag
     4681 ccgttcgact tcatactgct atcgacccac acctcgacgc ccgcgaacag cgcgcgctcc
     4741 ttgtcgggga gaaagtcagc gaggctgtgg tcgcgcagca gcaggtgcgc ggcttctctc
     4801 aggtgagtgc ggggttgcga gaacacggtc gggcgtccgc cccactcggt tcgcaggacg
     4861 tagcggcagg cgagcgcgcc ctgcgcggcc agcgccagcc acagcgcgca ggcgagcgag
     4921 tacttcaagg cggcgcgcat tctcacattc gcgcgcgcgg ccgacgcggc gagcatgagg
     4981 acgagcgcgc cgccgagcag ctcaaggtgc agcacgtagc ggacgtagcc cgtcgtggcg
     5041 gcccaggtga gtgagtcgag cagcacgacg aggcagaggg cgcgcacgag agcgtcgcgg
     5101 cgggcgagga ctaagcctgc gagcgcggcg acgaagccga gcgcgagccg gcctgaatag
     5161 acgttcaact ccgagagccg ttcgggcgtg aacacagagg cgacgggcca gagaagcttt
     5221 ccccaccacc cggccgccgc cggcccccag cgcgggtcgc gccagtcctg cgcggcccag
     5281 tagggcgact cgaagatcgt gttgaagagc gggaagacgg ggctgcccgt ctcgccgtag
     5341 atgtagaagg tgaacggcag gagcgggacg aggaacgccg ccgccgcgag ggcgagcgag
     5401 agcaaaatct ccctgcgcgc cggtcggagt ttgaacagaa gttgataggt gtaaatgagc
     5461 gcgatgaagc acgcgaccgt caggttcgtg agcttgaagg cgacggccac tccgagcaaa
     5521 agcccgagac gcgcggccgc aacgcgcacg cttccccgtt ctccgtcgcg tcgcagcgcc
     5581 acgtagaccg cttcgaggat gagcgggagg gcgagcacgt caaccatgta gttgttgatc
     5641 tcgaacagag agtgctcgca gagcatgatg agcagcacgc cgaacgcgcg ccgccgcgcg
     5701 ccgctgatgt aggggcgcag cagtctctcg acgacctgag cggcccagac gagcgccagg
     5761 aggttcggca gggtgccgag gcggtagccg agcaggcgtc ggcagaggcc cgtcacgatg
     5821 tcgggcgagg ggttgaacgg gatgggcgct gggaagaagt cgccgggcat gaagagcggc
     5881 cccgcgagcg ccctctcggc gtggagcagg cggtagttga gcacgtcgaa ggacacgtcg
     5941 gggaagggca cgcgcagcgc gtagacgaag gcgaggggca gggcgacgac gagccagaag
     6001 gcgggcggcg ttctttctct ctcctccccc gtcgcgacat agaaatacca ggcgagcgcc
     6061 gcgagcagaa gcgtcagcgc ccacgccagc ctctggtcgg cgacggccca caggtactcg
     6121 cgcgcgaagg ccgccaggta cagcagcatc aaggcgtcgc cgagccccac gcggcgcagg
     6181 aagtcccgcg ccctccgcgg caatgcggcg cgcgctatca caggtgcttc agacatttga
     6241 aatgtcggcc ctcgcccgcg cgtgctccgc cgccgacgct ccggcgcgcg cgcgggccgt
     6301 gatttcttga cttcggattt cagggcgcag acaatgccac ggcggttcgt ccggaagagg
     6361 tgtcgaggca tcgacgacgc cgccccgaag cgccgcgtgt gtttctgaaa aggcgctaga
     6421 ccctaacacc tccgcgtgga aacgccaacc gtacaagccg cccccgcgaa tgtcaaaacc
     6481 ggccggggcg cgtggaaaac gctgctcggc cggctggccg gagcgcctcg cgcgcgcgag
     6541 gcgctcgtca tcctcggctt cttcgccgtg acggccctga tgacgtggcc gtgggtgacg
     6601 cgcatgcgcg acgccgtcgg cgacgtgggc gacccctaca tgatcgcctg gacgctctgg
     6661 tgggactacc accagacgtt caacgacccg ctgaacctct ttcacgccaa cgtctttcac
     6721 ccctaccgct acacgctcgc cttcagcgaa aacgactacg gcatcgcgct cctcttcttc
     6781 ccgctctacg cgctcggcgc gcgcccgctc accgtacaca gcgtggcgac cttttgcggg
     6841 ttcgtcttct gcggctacgc ggccttccgg ctggcgcgca cgctcacggg ttccgtggcg
     6901 gccggctggg tggcgggcgt cgtcttcgcc ttcgtcccct accgcttcca cctgctctcg
     6961 cacctgcact acctcttcgc cgggtggatt ccgctgctgc tcgaagccct ggtgctgttc
     7021 gcgcgcgagc ggagttggaa gcgcgcggcg tggctcggcg tcgccttcct gatgaacgcg
     7081 ctgacgtgca tcacttggtt catcctgacg ctcccggcgc tggcgctgtc ggccgcgttt
     7141 ctggtcgcgc gctataggct cgcgcgggag cgggccttct gggtgcgcgg cgctgcggcg
     7201 ctcggtctcg cctcgctcgc cctgctgccg ttcctcctgc cttactacca cgtgagccgc
     7261 atgtacaact tcgcgtgggg ccgcgaggag gtcgtcaagc agtcgccgcg agtctcgacg
     7321 tggctgtctg cggagacgcg caaccgggtc tggaggtggc tcggcgcgcg ctccgacggc
     7381 tcgccgctct ttccgggcct gctcccgctg ctgctcgcgc tcgccgcctt cacgcgcctc
     7441 ggcgcgcgcc ggggcgacac cgcggcggcc cttgagggag tcgcagttga aaaggactcg
     7501 gcgacggctc ggcgcgctga gtggtggacg gagcttctgg acgcggcggc gctcgcggcc
     7561 ggggtgctgg cactcgtcgc cgccgtgttc gagggcacgc aaggatgggg gggaaccgtc
     7621 ttcggcgtgt tcacgtccga ccgcgcgctt tttgtgctcg cctgcgcgct ggtcgctcgc
     7681 ctctgtgtag cctatccgct ctggttccgg ccgtcgggga atttgattga gacgatgcgc
     7741 gccggacggc actccgacgc cgtctggctg ggcgcgctgt gggcggcggt cggcttcctc
     7801 atgtcgctcg gcacgaacag cctcttttac cggctcgtct acgaccacct ctttctcttc
     7861 cgcagccagc gtcttcccgc gcgcggggcg atgctggctt acgtcggcct cgccgtcttg
     7921 gcgggcctcg gcgcagtgcg agtcgccggg tgggtcgcgc gtcgtcgtcg cgacgtgagc
     7981 gccggggccg tctgcgccgt cctgatcctc gcgctgctgt tcgagctgcg cgcggccccg
     8041 ctcaggttcg tgcgcggggc ggtcttcccc gacgaggtca cgctgcgtct gaaggagacg
     8101 ccgatgcgcg gcggcgtggt ggagctgccc gccggccccg acggccacaa ccacctctac
     8161 atgctgcgcg ccgccgacca cgcccgcccg ctcgtcaacg ccacgtcgag cttcgtcccc
     8221 cagcccatct gggagttgca cgaactgacg cgcggcggcc ccatagctcc gcgcctgctc
     8281 gacctgctcg aagagctttc gacctcctac gtcgtcgtcc acaacgcgcg gctcgcgccc
     8341 gagcgtcaca ccgactacga acaattcttc gcgcgcgccg tcgccacggg gcggctgcgc
     8401 ttcgtccgcc ggtttgaagg acgcgacgac ctctacgccg tgacgatgtt cgagccggag
     8461 gcgcggggcg aggagcctct gccgttcgtc tccacgatgc gccgcctcga cgagatgctc
     8521 gccgaagacc cggttaacct gctcggcgag tttttcgagt ggggccagac gctcgaacgt
     8581 cttcacaagg cgtctttcgg acgcccgccg cgctacgagg agttcatgcc cgacgcgcag
     8641 cgactcgccg ccggcgtcct cgcgggcgac gaagacggcc gcacgcaact ggaaaataac
     8701 taccgcgcgc tcatctcgga ctggacgcgg cgcgcggcgt tcgtctcatc cttcggagcg
     8761 ttgagcgacg aacaatttat cgacgcgctc gcccgcaacg ccggcctctc gcccccggcc
     8821 gatgagcgcg cggcgctcgc cgaagctctg aggtcgggca cggagacacg cgcgggcgtc
     8881 ctcgccagcc tcgtcgcgcg tcgcgagttc gttgagcgtg agaactaccc gtcaaccgtg
     8941 ctgctgcact acttcggcta cctgcgacgc gaccccggcg ccccgcccga acgggacgcg
     9001 agcggcttcg acttctggat cacagagctt gaacatgcgg gcggcgaccg cgcgcgcctc
     9061 ggccgcgcct tcgccggctc cggcgagtac ctcgaactgc ggaagcgtct gggcaagcag
     9121 ccataaggcg actcactcgg cgatgtaggc tcggaaggct gcgcgctcgg agatgatgcg
     9181 gcagttgtgg cagggtagct cggtgctgat gacgagcgcg ccggcgggcg ggcgggcttc
     9241 tttggggagg cgcaggaacg cgcgcgcggg gtctgtgccg gcgagggtgg cgtaccagta
     9301 ggcgtggatg tagccggggg cgccgttgtt gtcgatgagg tggacgggcc gctcgggccg
     9361 gctcatcgcg gcggcgaaga cttcggggta gtgcgcgtcg aagctgtgcc agcgcgcggg
     9421 cgcggcctga cggaagtgcc actggaagag cgcgccctga aggagcgtcg tgaggaggag
     9481 ggcggcgaag acgacgcgac gcgcgcgcgt gtgtgcgccg ggcgtgagca ggcgctctag
     9541 cgcgggcgcg gtgagcagca ggaggaagac ggggacgccg acgagacgca gcgtgtggaa
     9601 gtgatcgagg gtcagcgccg acgggagcgc cgagacgagc aggccgtaga cgacgaagcg
     9661 ccaccaggcg tccgcgccgc gccgccggag gagaagcact aaccccgcgg ctgcggcgac
     9721 gacggtcggc gcgagcaggc tccccatcgt ctgcacgtgg tgacgcggct ccgggtcgcc
     9781 cgcgacgagc cagccgcgtg ggctgaagtg gcccgcgtag ttctgacaga accgcagcgc
     9841 cacctccatc caactcatcc ccggcttgat gaagctcacc tgcgcgaacc tctcgccgag
     9901 tgcgccgggc tgttcgacga agaagacgag gagcggcaga agcgtcacgg cgtagaggag
     9961 ccacgtgcga aaggccacgc tccaccagcg cccgcgcccg gcgaagagca ggaggccgag
    10021 cgcgaggagc ggcgcgagca gacgcccgac cgagtaagtg tacgtgatga gcgcgagcgc
    10081 gagcgccaca cgcacgttgt cgccgaacgt ccagcgcccg cgctcgtgcg cggagcgcag
    10141 cgcgagcaaa aggagcgcga gcacgagcgg catcaacgcc acttcaaaca cgagccggct
    10201 cagctcgaag agccacggcg tcaatagcgc cgagacggcg accgtcagcc cgaccgcacg
    10261 cggacgcacg ctcacactcc gcgcggcgac gagtccgagc aggagcgcgg ccgccgcgcc
    10321 aaggacggcg ctcagggagc gtgcgacggc gatgctcggc ccggtgagtt tgaacagcgc
    10381 ggcgaggaga tagacgtaga cggggctttt gtattcgccg aaggcgcgga agaagagggg
    10441 gaagcgcgcg ccgtgctcgt ccgcgccgtg gcgcgcgacg aggtgcgcgt tgtaggcgat
    10501 ggaggattcg tcggcgaaga agccgggcgg gttctcaggg acgctgtgca ggtagaggac
    10561 gaggcccaga cctgcgagcg ccgcccacgc gagcgcgccg ccgcgcggcc gtcgccgccg
    10621 cgccgagttg ccttccatct cctcggacat cgccgacgat tatgccgcag cttcgcgaaa
    10681 aggaaagctc cggtataatg tcgcgccgca cgggggttga tttcagttga gtataacgtg
    10741 aagcaaactc accgcagagg cgcagttaac gcagaggggg cgcggaggct tgacgcttta
    10801 tgacggcgct gctgtttctc gcgggcgcgg c
//
LOCUS       DASVES010000474        28434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1060390, whole genome shotgun sequence.
ACCESSION   DASVES010000474 DASVES010000000
VERSION     DASVES010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 28434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28434
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060390"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VF240_19735"
     CDS             <1..1036
                     /locus_tag="VF240_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177500.1"
                     /translation="KNDAQDASSRGSREQLAERKDALADSVGNLERDIEQSARSLGEG
                     RKQAARQLGEAAESLRRDRVAERIRNGKRGLESNQLEGARTEERAVERSLNELAERLQ
                     AAERAAGRPSGQQSGEEALDRTRRLADDLESLRRRLDDANARRGEGRDGQQQRQQGGQ
                     QQAGQQQGGQQKGGQQKGGQQSGGQKGGQSRAGQQQQGSPSQSGQQPGGQPGQGERGQ
                     AGEQMGGVGGDARQLGSELRERLREAEDLRSEWGSTSGISVGKLGAVVEQLRRMADGR
                     MQGDAQTAAALRAQVIEPLRQLELELSRRLREQLGRTNLRLHDEGAAPERYRKSVEEY
                     YRRLSGGGRR"
     gene            1044..1943
                     /locus_tag="VF240_19740"
     CDS             1044..1943
                     /locus_tag="VF240_19740"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX7177501.1"
                     /translation="MAGRRPQGQSPHDRLRRALKLARRAALPVALVALVLMPAAGQRR
                     RRGGGTTTPPELSVAAERPRNTELPTDGPAGPAGEFTFARAIYTSPFSPYWTRYGGSW
                     TVDYPEADNHFITGMREWAGTNLNITQRPEAIELLDERLFNYPFIYFVEPGFMELSPE
                     EAARLREYILRGGFLFLDDFWGESEWQNVQTQLKKVLPEYEIKELPLTHPILHSYLDI
                     EEVVQVPNIYNAQRGITGEKGGNTPYYMGIEDKSGRLVVFISRNSDLGDAWEWINDPR
                     YPVKYGLAAYRIGINVVIYAMSH"
     gene            2245..4113
                     /locus_tag="VF240_19745"
     CDS             2245..4113
                     /locus_tag="VF240_19745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7177502.1"
                     /translation="MRDTRALIDRVTAADAPHAERREAFGELVSRFQDMAYGCAYAVL
                     ADSYLAEDAAQEAFITAWQKLGQLREPAAFPGWLRRIVLTQCNRMTRGKRLVFLPLEA
                     GALVSAAEPGPQDAAESRELRAKVLAALRSLPEGERITTTLFYVGEYTQEDICAFLEV
                     PLATVRKRLFTARRRLRRSMVESFKDGLRDERPSRDDSFAEMVGARLRPFAQTDWELL
                     SALAHTQPGAEAQASDTWLAERRRFDESRRVRRHYVAEHAGTGRPLGYGAVEQSVYGP
                     RYRLHLAADPRHLRGGVGRLLVERLTADLREVNAVTVSLRGDASRPELLALLEGHGFV
                     ETSRVLDLRLKVSDAQDLAPLLPVAEELARRRVRITTLAEERKRDPDHLEKLYELRCA
                     AELDDPARSPLRPPAFDREEAALWLRLPYVLPDAYFIAVHGDQYVGVCDLNLRETAPG
                     GVTHGFTGVRREWRRQRVATALKARAVEYARSHGYRTVRAFNSPAHGAVLALNEKIGF
                     RPRLSLVTLEKCLREVKKVEPALYDAYAGRYRGGDDGPRPDFVLTIAKEGERLTAEFV
                     GQKVELFPESEASFFIKHFYGEVVFARGDDGRVTHLLWRERGANNTAREMRAAKID"
     gene            4314..5492
                     /locus_tag="VF240_19750"
     CDS             4314..5492
                     /locus_tag="VF240_19750"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7177503.1"
                     /translation="MRLRLLTVLLSVTALGSVSFSAKSERGTGLPDFAATPAQSPAPQ
                     SQTRPAHAQEQAAEQVFKNIQVLKGVPASQIQPTMAFITGALGVRCSHCHTSSAFERD
                     DKATKQTARRMMRMVLEINRGNFDGAAAVSCNTCHRGQTRPQSVPTLGRSLWLPSAAV
                     AKPDAAAAALPTVEQILDNYVRAAGGREALAKITSRVFKGSRVGADGVLVPEEVQQKA
                     PNKLLVTTSYPQIVFRRGFDGTMGWARDSQGGGDIGKEELAELAREAEFYKEVRLREL
                     YAGMSVEGRATVGEREAYVIVATTREGASEKLFFDTQTGLLVRKYTEIKLVLGAFPTQ
                     TDYEDYREVDGVRVPFVIRWSIPGRAWGRKIEEVKTNVHIDDATFRPPAAAGGERRPQ
                     "
     gene            5885..7405
                     /locus_tag="VF240_19755"
     CDS             5885..7405
                     /locus_tag="VF240_19755"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX7177504.1"
                     /translation="MDKQSAPDEARAAEATQPRMATGNEGLDKVLNGGFPANRLYLVE
                     GDPGTGKTTLALQFLLEGARRGEPVLYVTLSETKEELTEVAHSHGWSLDGVTIYELIA
                     PEESLKTETQYTIFHPSEVELGETTRAVIDEVERLQPRRAVFDSLSEMRLLARDPLRY
                     RRQILALKQFFSGRQCTVLLLDDRTSTSGDLQVQSIAHGVLALEQMAMEYGAERRRLR
                     VVKLRGSRYRGGFHDFNIQTGGIEVYPRLVAADHRQAFVQESISSSVPELDALLGGGL
                     DRGTSTLVIGPAGCGKSSISAQFLACAARRGEFSAAFLFDESANIHMARAAGLGADMR
                     SHVEEGRIRIQQVDPAELSPGEFAHRVRRVVETDGARVVVIDSLNGYLQAMPDERFLT
                     IHMHELLTYLNQQGVVTLVVMAQHGFMGDMATPVDVSYLADTVVMLRYFEARGAVRRA
                     ISVVKKRTGFHEDTIRELRLSSKGIEVGAPLTKFHGVLTGVPTYVGENGSLSKEGD"
     gene            7402..9072
                     /locus_tag="VF240_19760"
     CDS             7402..9072
                     /locus_tag="VF240_19760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177505.1"
                     /translation="MTIPDADNADERVLILAPTGRDASMTEKYLEEAGVGVESCVHIE
                     ELCERMLASAGAALISEEALTSEALECLGGALNQQPPWSDFPLIVLTSGADITPDNLG
                     TLKTLGEAGNMTLVERPTRVITLVSAVRSALRARRRQYQVRDHLNAERRAQQEAVEAR
                     QQAEESNRAKDLFLATLSHELRTPLTAVLGWARMLSSMKVDEETAHHGLQVIERNAES
                     QHQLIQDLLDVSRIITGKLRLDVRPVELASVVEAAVDSVQQAIEARAIDLNVEFDTQS
                     HLVSGDPDRLQQVVWNLLSNAIKFTPKGGRVGVRLDRRGSDVRLKVSDTGQGIAPEFL
                     PHVFERFRQADGSTTRAHGGLGLGLAVVRHLVEQHGGTVSAESAGAQQGATFTVNLPI
                     TAVSTPPEKVESKAGDGAGREQQPNGSNLLDGVRVLVVDDQPDARELLSMVLQYAGAE
                     VLTADSATAALDTLRRTRLDVLVSDIGMPDEDGFHLIGRLRSLPTEEGGATPAIALTA
                     YATEEDRQRSISAGFEDHLPKPVEPAELVAMVAQLAARKTGRASAVNE"
     gene            9119..9706
                     /locus_tag="VF240_19765"
     CDS             9119..9706
                     /locus_tag="VF240_19765"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HEX7177506.1"
                     /translation="MRNRKQGSRRREGDVLKGLVAGVAGGLAAAFVMNQFQSTWGKLT
                     EGVDKPHGAQSLQQGSPEHGVGRELREQGSDEQQDDATERVANAISTGVFDHELTRAE
                     KRRAGAAVHYAFGVTTGAMYGALAEVAPAVTTGAGLPFGTLVWAAADEGLVPALGLSK
                     SPTEYPLSIHAYALASHLVFGLTAEMVRRGVRGVI"
     gene            10295..11287
                     /locus_tag="VF240_19770"
     CDS             10295..11287
                     /locus_tag="VF240_19770"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177507.1"
                     /translation="MTAGLGCDGDTVSITAATQPSVEDVVMGAIPGLPKVHLHNPVLA
                     YEVGDDFMKWFYQAEQGLIDPYVLVVEGSIPNEKIKEEGYWAAQGNDPMTGQPITTCE
                     WIDRLAPKATAVVAIGTCATYGGIHAMAGNPTGCMGLADYLGWDFKSKAGLPIVNVPG
                     CPVQPDNFMETVLYLLYQLAGLAPMIPLDDQLRPTWLFGKTVHEGCDRAGYYEQADFA
                     TEYGSPKCIVKLGCWGPVVNCNVPKRGWMAGIGGCPNVGGICIGCTMPGFPDKFMPFM
                     DEPPGAKISAGAIGVYGRMIRRLRAITNTTVNKEPKWRHTGDKLTTGYRPTTYD"
     gene            11301..13109
                     /locus_tag="VF240_19775"
     CDS             11301..13109
                     /locus_tag="VF240_19775"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX7177508.1"
                     /translation="MSTTTIVGTKAPAKPRTLVDMNWDPITRIVGSLGIYTKIDFANK
                     EVAECYSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHATCSNYAQNMAFNVRP
                     PALAEWIVNLGEAAEYMFDHNIFQDNLVGVDFCEQMVRETNPSVWEKAKNTEAPHAAD
                     HGYRTIADIMTALNPFTGEFYRETLHVSRYTREMFCLMEGRHVHPSTLYPGGVGTVPT
                     VQLFTDYIVRLMRYCEFMKKVVPLHDDLFDFFYDALPGYEKVGERRILLGCWGSFNDP
                     NVCDYRYENMDEWGNAMFVTPGVIVDGKHVTTNLVDINLGIRILLGSSYYDDWQTAET
                     FVKTDPLGNPVDQRHPWNQTTTPRPQKRDFDNNYTWVMSPRWYHEGEQGGGSKYLALD
                     TGGGPIARLWSTALAGKVDIGYIKATGHSVKIYLPQTALKPEVEFEWKIPKWSNTIER
                     DRARTYFQAYAAAAALHFAEQALAELHAGRTKTWEPFTVPDEAIGCGFHEAVRGVLSH
                     HVVIRDGKIANYHPYPPTPWNANPRDTYGTPGPYEDAVQNTPLFEENGQDNFKGIDIM
                     RTVRSFDPCLPCGVHMYLGNGKVLETRHSPMFGAMK"
     gene            13199..14086
                     /locus_tag="VF240_19780"
     CDS             13199..14086
                     /locus_tag="VF240_19780"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX7177509.1"
                     /translation="MESQQLIQRIEELVRKIEATADPEARSTGVELLQSLMELNGAGL
                     ERMMEMAAETGAAGHALIDGFGRDELVRGLLLLYGLHPVALETRVAEALVKVRPYLQS
                     HGGNVEVLGIEDGVVRLRLQGSCKSCPSSAMTLKLAIEEAIYEAAPDVTSIEAEGVLE
                     PPVASSGLVPLGRLRSEEAAPPQPNGNGHGWQEVSGLAALAQGAVQTIAVSGRALLFC
                     RLDETFYAYSNDCPGCGEALQEAYLEATSIVCPACRQRYDCVGAGRGLDQPNLQLEPF
                     PLLMEQGRAKVSLPHLAAS"
     gene            14165..14401
                     /locus_tag="VF240_19785"
     CDS             14165..14401
                     /locus_tag="VF240_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177510.1"
                     /translation="MDEMDKEHDSKSESPAHDWGTGKGEDKSSTEGKEAGRHDTGATH
                     ADRPAGTSTARDSTSINPENMDPIDPNSPNMPPA"
     gene            14481..15104
                     /locus_tag="VF240_19790"
     CDS             14481..15104
                     /locus_tag="VF240_19790"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEX7177511.1"
                     /translation="MSESSRDRGEQSPLAALRRFVRPRAANVERCEMCSAALAAEHQH
                     LVEPSNRQMVCACEPCAILFSGQTGTKYKRVPRRIRVLPDFRLTDGQWDSLMIPIQLA
                     FFFHSTPDGKVLALYPSPAGPTESLLALDSWNEVVRENPVLREMEPDVEALLVNRVGG
                     GGEYYLVPIDECYKLVGLIRAHWRGLSGGTEVWREIKQFYAGLNERA"
     gene            15217..15894
                     /locus_tag="VF240_19795"
     CDS             15217..15894
                     /locus_tag="VF240_19795"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEX7177512.1"
                     /translation="MPDLNFQVEGAEAVAYAAAPLLSFKLRVTNAEGDEPIHTVALRC
                     QIQIETTRRRYNEREQERLLDLFGEPERWGQTLRTMLWTHASMVVTPFQGSTLVELPV
                     PCTFDFNVAATKYFAGLEDAEVPLALLFSGTVFYEAADGALQVSQISWEKEAKYQLPV
                     SVWQQMMNHYYPNSAWLSLRRDVFDRLSRYKMRRGIPTWEQALESLLPAVEETGEARE
                     IEEKIQA"
     gene            15891..16991
                     /locus_tag="VF240_19800"
     CDS             15891..16991
                     /locus_tag="VF240_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177513.1"
                     /translation="MNLERVEKIANAVLYEGYILYPYRSSSVKNQQRWNFGALCPPAY
                     SEAQGGTESCQMQTECLVEGAEHAALDVKVRFLHLLTREVRRLTTPLPELPEGAEPDF
                     QRVAAMEVGGRIYQTWQEAVEREVDLPGINLSKLDAEGQRQTFTFPSKQETEPLRESD
                     GQTVGVLVRKQQLIEGAIEVVAEQAREGLFKVTVRILNLTPLEDAATRSRDEALMRSL
                     VSTHTILGVREGGGEFVSLLEPPEAFREIAASCRNTGTWPVMVGEEGARDCMLSSPII
                     LYDYPQIAPESAGDLFDGTEIDEILTLRIMTLTDEEKREMRGVDERARRILERTEQLP
                     AEQMMKLHGGIKKVQSSKFKVQSEEPVSDFEP"
     gene            17023..17352
                     /locus_tag="VF240_19805"
     CDS             17023..17352
                     /locus_tag="VF240_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177514.1"
                     /translation="MNEWEFQLLEERTLVEQVEVGGAHIKTGDRVRLRPRAGGDIFDL
                     ALAGQIAIVESIEQDYEDRVHLAVVLEDDPGQDLGRLRQPGHRFFFSPEEVEPLPAPG
                     GEDAKSG"
     gene            17349..17867
                     /locus_tag="VF240_19810"
     CDS             17349..17867
                     /locus_tag="VF240_19810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX7177515.1"
                     /translation="MTQPRILIAGIGNIFLGDDAFGSEVARRLAVRELPEEVRVVDFG
                     IRGFDLTYALLDGYEVTIFVDATPRGEAPGTLYTIEPNLSELDDLDAESMMVEPHGMN
                     PLKVLGMVKSMGGEFKRIFLVGCEPAPLCSEDGHMGLSEPVTAAVDEAVKVVESLVRE
                     ILEERGQKATAV"
     gene            18202..18765
                     /locus_tag="VF240_19815"
     CDS             18202..18765
                     /locus_tag="VF240_19815"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7177516.1"
                     /translation="MNESSVVRYRIDAGRSRFTVQGVAEGMLSMFGHNPVIAICGFGG
                     DVRCDPGTLEAASLLMLVQTDSLAVVNKVSGKDKREMERGMREDVLEIARYPEVVFMS
                     TKVSANRTAEGQYQTRISGNLSLHGVTREHQINAQVTVNGSSLRAQGESALRQSDYNI
                     KPVSAVGGTLKMKDELKLSFDIIASEQ"
     gene            18834..19178
                     /gene="hypA"
                     /locus_tag="VF240_19820"
     CDS             18834..19178
                     /gene="hypA"
                     /locus_tag="VF240_19820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX7177517.1"
                     /translation="MHELSIAMSMVEMAEEEAARRGGVKVFAIHLKLGQLSGVVKDAL
                     LFSYEIVCQGTTLEGSQLIIEEIPVVVYCPTCRAETSLASLQRFCCAACGTLTSEVVR
                     GKELEVVAMEIQ"
     gene            19175..19867
                     /gene="hypB"
                     /locus_tag="VF240_19825"
     CDS             19175..19867
                     /gene="hypB"
                     /locus_tag="VF240_19825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX7177518.1"
                     /translation="MMSSQPRLVEVRQNVLKQNDLLARELRRRFQEAGVCVVSLVSSP
                     GSGKTAFLEKALTLLQRSKLRVAALVGDLATENDAARLARSGAPVRQITTGTICHLDA
                     QMVERALEEWNLEELDVLFIENVGNLVCPSSYDLGEDLRLVLLSVTEGEDKPLKYPTI
                     FNTADVAVITKIDLAEAVEFDRAAAHRNIQSVRPGMKVFEVSAKTGEGMDELLPVLYS
                     ARSAESEKVLAG"
     gene            19931..22222
                     /gene="hypF"
                     /locus_tag="VF240_19830"
     CDS             19931..22222
                     /gene="hypF"
                     /locus_tag="VF240_19830"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX7177519.1"
                     /translation="MQILVRGIVQGVGFRPYVYSLAARGALRGRVLNNTTGVLIDVEG
                     EDHRIEEFINEITLNPPPLSQIESIQRHDDLASANYQDFRIVESAAEGVKLVPVSADV
                     ATCADCLRELFDSQDRRHRYPFINCTNCGPRFTIVEAVPYDRAQTTMRDFEMCAPCRA
                     EYENPLDRRFHAEPTACASCGPRLRLTDACARAPEFDEADGLDIIGRVRRLLSSGKIV
                     AIKGIGGYHLACDALDAGAVERLRQRKYREDKPFALMGASVDVVREYCRVSELEERLL
                     LSARRPIVLLEKRAGAVLPKAVAPRVNTLGFMLPYAPLHHLLLEDLDRPLVLTSANVS
                     DEPICYEDAEARERLGAIADYFLLHDRRIHMRADDSVARVHAGREMILRRARGYAPAP
                     VRVAFRFERQILACGAELKNTFCLARDNYAFISHHVGDLENLETLRSFERGVEHYRRL
                     FHLNPDVVAYDLHPEYLSTKYALALDEIPDKIGVQHHHAHVASCMADNRIEGEVIGVA
                     MDGLGFGTDGRLWGGEFFVADFCDAERVAHLDYVPMPGGAKAVREPWRMAAVYLHRAF
                     GDEFLNLDIPFVQNLDRRAWTTLRRMAATATNSPETSSMGRLFDAVAGLLSLRGAVNY
                     EGQAAIELETIAERACRQEGYAFELEEGGRVIKAEAVVRRAVEDLLEGRRPQEVSAKF
                     HLGVAALIAAVARRVRDERKLERVVLSGGVFQNTFLLEAACRMLKGDGFEVFTHSRVP
                     PNDGGISFGQAAIADARLKAGRV"
     gene            22226..22492
                     /locus_tag="VF240_19835"
     CDS             22226..22492
                     /locus_tag="VF240_19835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX7177520.1"
                     /translation="MCLAIPGKIVEIIDEENSIAKVDVGGVRRNINTGMLTPDEARVG
                     NYVLIHVGFAMSKVDEHEAMETLRILEEIGQFDEEFDQFKTSLE"
     gene            22612..23313
                     /locus_tag="VF240_19840"
     CDS             22612..23313
                     /locus_tag="VF240_19840"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7177521.1"
                     /translation="MSESAKDFSRAFYPFLHADEKKPQELMEELRFSLLEKARESVEI
                     KSRFFEENKDTILAASLAMAKAFHHGRKLLVCGNGGSATDAQHVTVEFMHPVIVGRKA
                     LPAICLTSDMAMVTAVANDVGFDDVFTRQIIALGAEGDILLGISTSGNSENLRHAFAT
                     ARARRLVTVGFAGGDGGRMCELGAKGLLDFCLTVPTSSIHRIQETHVALYHIMWDMVH
                     TFMQHRSLVEEQAAE"
     gene            23532..24689
                     /gene="hypD"
                     /locus_tag="VF240_19845"
     CDS             23532..24689
                     /gene="hypD"
                     /locus_tag="VF240_19845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX7177522.1"
                     /translation="MKFVDEYRRGDIAERLAGQVAQLTERPLKLMEVCGGHTHTIFKY
                     GIEDLLPENITMIHGPGCPVCVIPLGRVDDAISIAQQPGVIFTTFGDAMRVPGSRTSL
                     LDAKASGADVRMVYSPLDALKIAKKNPERDVVFLALGFETTAPSTALTVLQAAKDGVE
                     NFSVFCNHITIVPALKALLDSPDLQLDGFLGPGHVSTVIGTRPYDFVPQEYGKPVVVA
                     GFEPLDILQSVYMLVRQVVEGRAEVENQYGRCVHRDGNRKALEVLFEVFEPRDFFEWR
                     GLGSIAHSGMKLSRKYAAFDAELRFSVPGVGVADPKACQCGEILKGVKRPWECKVFGT
                     ACTPETPIGSCMVSSEGACAAYYNFGRLSKIAERSAPNGESKRAVEAGVTV"
     gene            24693..25802
                     /gene="hypE"
                     /locus_tag="VF240_19850"
     CDS             24693..25802
                     /gene="hypE"
                     /locus_tag="VF240_19850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX7177523.1"
                     /translation="MTSEFNPSPLFERVERARRRKRKFKDEQITLAHGSGGRAMHELI
                     EGLFLEYLRNPVLEALEDQAVFEVGDGRGPARLAFTTDSYVVTPIFFPGGDIGRLAVC
                     GTVNDLCVSGARPLYLSAGFILEEGFAIEDLKRVLVSMRAAAEEAGVAVVTGDTKVVQ
                     KGSADKIFINTAGLGVIESPVQISAARAQVGDCVILSGAIGDHGTTIMIARGELELET
                     DIESDTAPLNSLVREMLDEAAGAGSLEGIHALRDPTRGGVATTLNEIALASEVCIEIR
                     EDLIPVREEVHGACEILGLDPLYVANEGKLVAVVAADIAERLVARMKQNTYGRAACII
                     GEVKAEPKGIVAMRTGFGGTRIVDMLVGEQLPRIC"
     gene            25921..26640
                     /locus_tag="VF240_19855"
     CDS             25921..26640
                     /locus_tag="VF240_19855"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177524.1"
                     /translation="MTSLSTVSVLAFGFVLGLRHAVEADHLAAISTIVSERKSILSSS
                     LVGGLWGVGHTISLVVAGVAVILLGVEIGARTALALEFCVGLMLVALGANALRKLARG
                     GRVHLHVHQHGGRAHIHPHVHGNSPEEAPHTHHGLRLDTRTLVVGMIHGLAGSAALML
                     LILSTIESATVGFLYIVVFGVGSIGGMMLMSALIGLPLYFTAARFNRANLALRGVAGV
                     FSLSFGLLMVYQIGFVGGLFR"
     gene            26698..27567
                     /locus_tag="VF240_19860"
     CDS             26698..27567
                     /locus_tag="VF240_19860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX7177525.1"
                     /translation="MKGRKVPAWVSVPLVVGAFGLLAWLERRRPLRRSVEPKLRREAR
                     NLAVAAVGAAALRVAEQPVVGPLSALVERRRWGLLKLWKLPVWLETALAVLLLDYTLY
                     VWHVLTHKQPWLWRFHLVHHLDLDLDASTALRFHFAELMVSVPWRAAQVLLIGVSPLS
                     LSVWQTFLFLSILFHHSNVRLPVEVERRLNSLVVTPRMHGIHHSLVKEETNSNWSSGL
                     TLWDRLHGTLRQNVPQDAIIIGVPAYRDPDEVTLPKLLRMPFGEERPTWALPEGATPR
                     RVAAPVAEDHLLA"
     gene            27585..27977
                     /locus_tag="VF240_19865"
     CDS             27585..27977
                     /locus_tag="VF240_19865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7177526.1"
                     /translation="MKQSIAHIALVVDDYDEAIKFYTEKLNFTLVEDTAQSETKRWVL
                     VAPKGAEECSLLLAKGVGEEQRSRIGNQTGGRVFLFLKTDDFWRDYKSMLSQDIVFVR
                     EPKNEEYGTVAVFKDLYGNLWDLIEFKK"
     gene            28019..>28434
                     /locus_tag="VF240_19870"
     CDS             28019..>28434
                     /locus_tag="VF240_19870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177527.1"
                     /translation="MDNRTTKPRPEGERPPALSIIIPTLNEARSIGQTLDAVMGAQGA
                     VEVIVVDGGSVDGTAEIARGRGARLVTSGRGRGEQMHAGACAARGSALWFLHADTRAS
                     AGCAEAVAGALRDPEVVAGNFSVRFDGDGRAARFLT"
BASE COUNT         5435 a         8888 c         9157 g         4954 t
ORIGIN      
        1 aaagaacgac gcgcaggacg cctcgtcgcg tggaagccgc gagcaactgg ccgagcgcaa
       61 ggacgcgctc gccgactccg ttggcaatct tgagcgcgac atcgagcaga gcgcgcgctc
      121 tctcggcgag ggacgaaagc aggccgcaag gcagttgggt gaggcggcgg agtcgctgcg
      181 tcgcgaccgc gtggccgagc gcatccgcaa tggcaagcgc ggactcgaaa gcaaccagct
      241 cgaaggggcg cgcactgaag agcgcgccgt cgagcgcagc ctcaacgagc ttgcggaacg
      301 actccaggcc gccgagcgcg ccgccggccg cccctccggc cagcagagcg gcgaagaagc
      361 gctcgaccgc acgcgccgcc tcgccgacga cctcgaatct ctgcgtcgtc gtctcgacga
      421 cgccaacgca cgacgcggcg aaggccgcga cgggcagcag caacgacagc agggaggcca
      481 gcagcaggca ggccaacagc aaggcggaca gcagaagggt ggtcaacaga agggcggtca
      541 gcagtcgggt ggacaaaagg gcggtcaatc gcgcgccggg caacaacagc agggcagccc
      601 gtcgcagtcg gggcaacagc cgggcgggca gccggggcag ggcgagcggg ggcaggcggg
      661 cgagcagatg ggcggcgtcg ggggcgacgc acgtcagctc ggctcggagc tgcgcgagcg
      721 tctacgcgag gccgaagatt tgcgtagcga gtggggcagc acgagcggga tatccgtggg
      781 caagctcggc gccgtcgtcg agcagttgcg gcgcatggcc gacgggcgga tgcagggcga
      841 cgcgcagacg gccgccgcgc tccgcgcgca ggtgatcgaa ccgctccgcc agctcgaact
      901 ggagttgagc cgtcgcctgc gcgaacaact cgggcgcacc aacctgcgcc tgcacgacga
      961 gggcgcggcg cccgaacgct atcgcaagtc ggtcgaagag tattaccgtc gcctctcggg
     1021 cggcggccgg aggtaaacag caagtggcgg gacgaagacc acaagggcag agtccgcacg
     1081 acagactgcg acgcgcgctg aagttagcgc gacgcgcggc gttgcccgtc gcgctcgtgg
     1141 cgctggtgtt gatgccggcc gcgggtcagc ggcgtcggcg cggcgggggc actacgacgc
     1201 cgccggagtt gagcgtcgcg gccgagcggc cgaggaacac ggaactgccc acggacggcc
     1261 ccgccggccc ggccggtgag ttcaccttcg cgcgcgccat ctacacctcg ccgttctccc
     1321 cctactggac gcgttacggc ggctcctgga cggtcgatta ccccgaggcc gacaaccact
     1381 tcatcacggg gatgcgcgag tgggccggca ccaatctcaa catcacgcag cggcccgagg
     1441 ccatcgagct tctcgacgaa cggctgttca actacccgtt catctatttc gtcgagccgg
     1501 gattcatgga gctgtcgccc gaggaggcgg cgcgtctgcg cgagtacatc ctgcgcggcg
     1561 gcttcctctt cctcgacgac ttctggggcg aatcagagtg gcagaacgtc cagacgcagt
     1621 tgaagaaggt gctccccgaa tacgagatca aggagctgcc gctcacgcac ccgatactgc
     1681 actcctacct cgacatcgaa gaggtcgtgc aggtgccgaa catctacaac gcgcagcgcg
     1741 gcatcaccgg cgagaagggc ggcaacacgc cctactacat gggcatcgag gacaagtcgg
     1801 gccgcctcgt cgtcttcatc tcgcgcaact ccgacctcgg cgacgcctgg gagtggatca
     1861 acgacccgcg ctaccccgtc aagtacggcc tcgcggccta caggatcggc atcaacgtcg
     1921 tcatctacgc aatgagccac tgacggccgc ggccccttca gccttccaaa gttcttagac
     1981 tggctctccg cttgagtgtc aggcacggcg gttcaccgcg gaggcgcgga gggcgcaaag
     2041 cgaaggcaaa agtaaaaaga caaaaggcaa aagtgaagag gctttcttac ttttgccttt
     2101 tgtcttttgc cctttgcctt cctccgcgtc ctctgcgcct ccgcggtgag tccgaccctc
     2161 cacacactca agtaagtttt tacgcacacg gtaatctttc cccgcccacc tggctcttat
     2221 cttatagaga cggcagattt cgacttgagg gacacgcgcg cactcatcga cagggtcacc
     2281 gccgcggacg ccccgcacgc cgagaggcgc gaggcgttcg gcgagctggt gtcgagattt
     2341 caggacatgg cctacggctg cgcctacgcc gtgctcgccg actcctacct cgcggaggat
     2401 gccgcgcagg aagccttcat cacggcgtgg cagaagctcg gtcagctccg cgaacccgcc
     2461 gcgttccccg gctggctccg ccgcatcgtg ctcacgcagt gcaaccgcat gacgcggggc
     2521 aagcgcctcg tcttcctgcc gctcgaagcc ggcgcgcttg tctccgcggc cgaacccggc
     2581 ccgcaggacg ccgccgagag tcgcgagctg cgcgcgaagg tgctcgccgc cctcaggtct
     2641 ctgcccgaag gagagcgcat cacgacgacg ctcttctatg tgggcgagta cacgcaggaa
     2701 gacatctgcg cgtttctgga agtgccgctc gcgaccgtga ggaagcgtct cttcacggcg
     2761 cggcgacggt tgaggaggag catggtcgag tcgttcaaag acggactgcg cgacgagcgc
     2821 ccctcccgcg acgactcttt cgccgagatg gtgggcgcgc gtctgcgccc gttcgcccag
     2881 accgattggg aacttttgtc ggcgctcgcc cacacgcaac cgggcgccga agcgcaggcg
     2941 agcgacacgt ggctcgccga acgccggcgg ttcgacgagt cgcgccgcgt ccgccggcac
     3001 tacgtggccg agcacgccgg gacgggccgt ccgctcggct acggcgccgt cgagcagagc
     3061 gtctacggcc cgagatacag gctgcatctg gcggccgacc cgcgtcacct gcgcggcggc
     3121 gtcggccgtc tgctggttga gcgcctcacc gccgacctgc gggaggtgaa cgccgtcacc
     3181 gtctctctgc gtggcgacgc ctcgcggccc gaactcctcg cgctgctcga agggcacggc
     3241 ttcgtcgaga cgagcagggt gctggattta cgtctcaagg tctcggacgc gcaagacctc
     3301 gcgccgctcc tgccggtcgc tgaagagttg gcccggcgtc gtgtacgtat cacgactctt
     3361 gccgaggagc gcaagcgcga ccccgaccat ctggaaaaac tctacgagct gcgttgcgcg
     3421 gcggagctgg acgacccggc gcgctcgccc ttaaggccgc cggccttcga ccgcgaagag
     3481 gctgcgctct ggctgcgtct gccctacgtc ctgcccgacg cctacttcat cgccgtccac
     3541 ggcgaccaat acgtcggcgt gtgcgacctg aatctgcggg agacggcgcc gggcggcgtg
     3601 acgcacggct tcacgggcgt caggcgcgag tggcgtcggc agagagtagc cacggcgctc
     3661 aaggcgcgtg ccgtcgagta cgcgcgtagt cacggctacc ggacggtgcg cgccttcaac
     3721 agtccggcgc acggggccgt cctcgccctg aacgaaaaaa tcggcttccg cccgcgcctg
     3781 agcctcgtca cgctggagaa gtgtttgcgg gaagtgaaga aagtcgagcc ggccctgtac
     3841 gacgcttacg ccggacgtta ccgcggtggc gacgacgggc cgcgcccgga tttcgttttg
     3901 accatcgcga aagagggcga gcgtctgacg gccgagttcg tcgggcagaa ggtcgagctg
     3961 ttcccggagt cggaggcgag cttctttatc aaacattttt acggcgaagt tgttttcgcc
     4021 agaggcgacg acgggcgggt gacacacctg ctctggcgcg agcgcggagc gaacaacacc
     4081 gcacgcgaga tgcgcgccgc gaagattgat tgagagcgtt tatagccagt tgctattgcg
     4141 tgcggttaga tcgctttgag ttgaatgtat ggcaagtcaa ctcaccgcgg aggcgcagag
     4201 gacgcagagt ttacgcggag ggttgacttc gctttcactc aagtgaacat cactccaata
     4261 ctccgaacca caggcggctt cgggaagaca tggttctgaa gagggctacg aaaatgcgtc
     4321 tccggctcct gacagttttg ctctccgtca ccgccctcgg ctccgtcagt ttttccgcga
     4381 agagcgagcg ggggactggt ctccctgact tcgcggcgac gcccgcgcag tcgcccgcgc
     4441 ctcaatccca gacgcggccc gcgcacgcgc aggagcaagc ggccgaacag gtcttcaaga
     4501 acatacaggt gttgaagggc gtgccggcgt cgcagataca gccgacgatg gcgtttatca
     4561 ccggcgcgct cggcgtgcgt tgcagccact gccacacgtc gagcgccttc gagcgcgacg
     4621 acaaggcgac taagcagacc gcccggcgga tgatgcggat ggtgctggag atcaaccggg
     4681 gaaatttcga cggcgcggcg gccgtgagct gcaacacctg ccaccggggc cagacccgtc
     4741 cgcagtccgt ccccacgctg ggccgaagcc tctggctgcc gtcggccgcc gtggcgaagc
     4801 ccgatgcggc ggcggcggcg ctgcccaccg tcgaacagat tctcgacaac tacgtgcgcg
     4861 cggcgggggg ccgggaggcg ctggcaaaga tcacgtcgcg ggtcttcaaa ggctcgcgcg
     4921 tcggcgccga cggcgtgctc gtgccggagg aggtgcaaca gaaggcgccg aacaagctgc
     4981 tcgtcacaac cagttatccc cagatagttt tccgtcgcgg gttcgacggc acgatggggt
     5041 gggcgcgcga cagtcagggc ggaggcgaca tcggcaaaga ggagctggcc gagcttgcgc
     5101 gcgaggcgga gttttacaag gaggtcaggc tgcgggagtt gtacgccggc atgagcgtcg
     5161 agggcagggc cacggtgggc gagcgcgagg cttatgtcat cgtggcgacg acacgcgagg
     5221 gcgcttccga aaaactcttc ttcgacacgc agaccggcct gctcgtgcgc aagtacacgg
     5281 aaatcaagtt agtcctcggc gcgtttccga cgcagacgga ctacgaagat taccgggagg
     5341 ttgacggcgt ccgtgtgccc ttcgtcatcc gctggtcgat accgggccgc gcgtgggggc
     5401 ggaagattga ggaagtgaag acgaacgtac acatcgatga cgcgaccttc agacccccgg
     5461 ccgccgcggg cggcgagcga cggccgcagt aactttactg aaagactctc gacaacgctt
     5521 gaagggccgc ccggataatt cccggcggcc cttcgcacgt ctgtatcagt tgccgtcgca
     5581 cacagtaccg cgagcggtag cgagcgggtg tggatgcggc agcggctcgt ctcgggcgcg
     5641 tcagacccgc tcgctaccgc tcgcggtact gatttcaatg gcgctcgctc gtcgcacccg
     5701 acgcgccgag tttcgcggga ggttggagac aaaagaatac ttttgtgttt tacttgtcgg
     5761 gcacccaatt caatctggcc ggtgtctccc gagttctacg gccctgacgg cggcttccct
     5821 tgcggaagaa tctttgaggc cggagccggc gatgagccga ccgaataccc gataggatca
     5881 atatatggac aagcagagtg cgcccgacga agcgcgtgcg gcggaagcga cacagcccag
     5941 aatggcgacg ggcaacgaag gactcgacaa ggttctgaac ggcggcttcc ccgccaaccg
     6001 cctgtatctc gtcgaaggcg accccggcac gggcaagacc acgctcgcct tgcagttcct
     6061 gctcgaaggc gcgcgccgcg gcgagccggt gctctacgtc accctctccg aaacgaagga
     6121 ggagctgacc gaagttgccc actcgcacgg ctggtcgctc gacggcgtca ccatctacga
     6181 gctgatcgcc cccgaagaaa gtctgaagac cgagacgcag tacacgattt tccacccgtc
     6241 ggaggtggag ctgggcgaga cgacgcgggc cgtcatcgac gaggtggaac gcctccagcc
     6301 gcgccgcgcc gtcttcgact cgctctccga gatgcgcctc ctcgcccgcg acccgctccg
     6361 ctaccggcgg cagatactcg ccctcaagca gttcttctcc ggccgacaat gcacggtgct
     6421 cctgctcgac gaccgcacct cgacgagtgg cgaccttcag gtgcagtcaa tcgcgcacgg
     6481 cgtcctcgcg ctcgaacaga tggcgatgga gtacggggcc gagcgccggc gcttgcgcgt
     6541 cgtcaagctg cgcggcagtc gctatcgcgg cggctttcac gacttcaaca ttcagacggg
     6601 cggcatcgag gtctatccgc gtctggtggc ggccgatcac cggcaggcgt tcgtgcagga
     6661 gtccatctcc agctccgtgc cggaactcga cgcgctgctc ggcggcggcc tcgaccgcgg
     6721 caccagcacg ctcgtcatcg ggccagccgg ctgcggtaag tcgtcaatct cggcgcagtt
     6781 tctcgcgtgc gcggcccgtc ggggcgaatt ctccgccgcc ttcctcttcg acgagagcgc
     6841 caacatccac atggcgcgcg ccgccggact cggcgccgac atgcgctccc acgtcgaaga
     6901 gggccgcatc cgcatccagc aagtggaccc ggcggaactc tcgcccggcg agttcgcgca
     6961 ccgcgtgcgc cgcgtcgtcg agacggacgg cgcgcgcgtc gtcgtcatcg acagtctcaa
     7021 cggctacttg caggcgatgc ccgacgagcg tttcctgacc atccacatgc acgagcttct
     7081 cacctacctc aaccagcagg gcgtggtgac gctcgtcgtg atggcgcaac acggcttcat
     7141 gggcgacatg gccacgcccg tcgatgtcag ctatctggcc gacaccgtcg tcatgctgcg
     7201 ttacttcgag gcgcgcgggg ccgtgcgccg ggcgatctcc gtcgtcaaga agcgcaccgg
     7261 ctttcacgaa gacaccatcc gcgagctgcg cctatcgtcg aaagggatag aggtcggcgc
     7321 gcccctgacg aagtttcacg gcgtgctcac gggcgttccg acctacgtcg gcgagaacgg
     7381 ctcgttgtcg aaggagggcg attgaccatc cctgacgccg acaacgcgga cgagcgggtg
     7441 ctcatcctcg cgccgacggg gcgcgacgcc tccatgacgg agaagtacct cgaagaggcg
     7501 ggcgtcggcg tcgagtcgtg cgttcacatc gaggagcttt gcgaaaggat gctggcgagc
     7561 gccggggccg ccctcatctc ggaggaggcg ctcacctccg aggccctcga atgtctgggc
     7621 ggcgcactga atcaacagcc cccctggtca gatttcccgc tcatcgtctt gaccagcggg
     7681 gccgacatca cgcccgacaa cctcggcacg ctcaagacgc tcggcgaggc cggcaacatg
     7741 acgctcgtcg agcgtcccac gcgcgtcatc acgctcgtca gcgccgtccg ctcggccctg
     7801 cgcgcgcgcc gccgccagta tcaggtcaga gaccacctca acgccgagcg gcgcgcgcaa
     7861 caggaggcgg tcgaggcgcg ccagcaggcc gaagagtcca accgcgcgaa agatttgttt
     7921 ctcgccacgc tctcgcacga gctacgcacg ccgctcacgg ccgtgctcgg ctgggcgcga
     7981 atgctgagca gcatgaaggt ggacgaggag accgcgcacc acggcctaca ggtcatcgag
     8041 cgcaacgccg agtcgcagca tcagctcatt caggacttgc tcgacgtgtc gcgcatcatc
     8101 accggcaagc tccgtctgga cgtgagaccg gtcgagctgg cctcggtcgt cgaggcggcc
     8161 gttgactccg tgcagcaggc catcgaggct cgggccatcg acctcaacgt ggagttcgac
     8221 acgcagtcgc atctggtgag cggcgacccc gaccgcttgc agcaggtggt ctggaacctg
     8281 ctctcgaacg ccatcaagtt cacgcccaag ggcgggcgcg tcggcgtccg actcgaccgg
     8341 cgcggctccg acgttcgcct caaggtgtcc gacacggggc agggcatcgc cccggagttt
     8401 ctgccgcacg tcttcgagcg cttccggcag gccgacggtt cgaccacccg ggcgcacggg
     8461 ggcctcggcc tcggcctcgc cgtcgtccgc catctcgtcg agcaacacgg cggaacggtc
     8521 tcggccgaaa gcgccggcgc gcagcagggc gccaccttca ccgtcaacct gccgatcacg
     8581 gcggtgagca cgccgccgga aaaagtagag agcaaagcgg gcgacggcgc gggccgcgag
     8641 cagcagccga acggttcaaa tttactggac ggcgtgcgcg tcctcgtggt tgacgaccag
     8701 cccgacgcgc gcgagctgct ctcgatggtt ctccagtatg ccggggcgga agtgttgacg
     8761 gccgattcgg cgaccgccgc actcgacacg ctccggcgga cgcggctcga cgtgctcgtc
     8821 tccgacatcg gcatgcccga cgaagacgga tttcatctga tcggccggct caggtcgctc
     8881 cccacggaag aagggggcgc gactccggcc atcgccctga cggcctacgc gacggaggag
     8941 gatcgccagc gttccatctc cgcgggcttt gaagatcacc tccccaagcc ggtggaaccg
     9001 gccgagctgg tcgccatggt cgcccaactc gccgcgagga aaacgggccg cgcctccgcc
     9061 gtcaacgaat gaatacactc ccaacgcctc tcggtaacag cagctcggag aacaggtcat
     9121 gcgaaataga aaacagggaa gcagacgccg cgagggcgac gtgttgaagg gactcgtggc
     9181 gggcgtcgcc ggcggtctgg ccgccgcctt tgtgatgaat cagtttcagt ccacgtgggg
     9241 caagctaacg gaaggggtcg ataagccgca cggcgcgcag tctctccagc aaggctcccc
     9301 ggagcacggc gtcgggcgcg agttgcggga gcagggcagc gacgagcagc aggacgacgc
     9361 gaccgagcgg gtggccaacg ccatctccac cggcgtcttc gatcacgagc tgacgcgcgc
     9421 cgagaagcgg cgggccggcg cggccgtcca ctacgcgttc ggcgtgacga caggggccat
     9481 gtacggcgct ttggcggaag tggctcccgc ggtgacgacg ggcgccggcc tcccgttcgg
     9541 cacgctcgtc tgggccgccg ccgacgaggg tctggtgccg gcgctcggcc tgtcgaaatc
     9601 accgaccgag tatccgctct cgatccacgc ttacgccttg gcctcacacc tcgtcttcgg
     9661 gctgacggcc gagatggtgc ggcgcggcgt gcgcggcgtg atctgaaacg cgcagacgag
     9721 aataatgcaa tagtctgttg ataactttca ggcactccgt aaacgccggg cggcaatgtg
     9781 ctgtaagaca cattcgggtc tccttctaac tattgacacg aaggaaaacc cggcgttaaa
     9841 gtagccccac attgaaaata acgtaagcgg tagcgaagtt ggattgattt tctcagcgaa
     9901 gaattcatcc agttacggag cgacccgaca ccctccgcag aaatgctgcg ctgaaccctt
     9961 ctctgaatct ttattcctcg cggactgttc tacgatcccc catcgttact gccgcaactc
    10021 ccccggttgc ggtaaagaac tctccatctt tacgtgtgtc ctcaagttac gtagcgagtc
    10081 aggttgctgt tgtaatcata ggtgggccta agagcctgac tctgatgtga gcagaagctg
    10141 atggtcgcgc cgtgtttgcc aaagcaaacc gacccaccac caaaccagtt acatcaactg
    10201 actgaaagga gagacagcga tggcagcaga aattgctcca tacggtcggg tcactcagcg
    10261 gccacctgcg gtcaaggagg tccacattct gtggatgact gccggtctgg ggtgtgacgg
    10321 cgatacggtt tccatcaccg ccgcgactca gccgagcgtc gaggatgtcg tcatgggggc
    10381 gataccggga ctcccgaaag ttcatctgca caaccctgtc ctcgcttatg aagtcggcga
    10441 cgacttcatg aagtggtttt atcaggccga gcagggcttg atcgaccctt acgtgctggt
    10501 cgtcgaaggc tcgattccaa acgagaagat caaggaagaa ggctactggg cggcgcaggg
    10561 aaacgatccg atgacgggac agccgataac cacctgcgag tggatcgacc ggctggcccc
    10621 gaaggccacg gccgtcgtcg ccatcggcac ctgcgccacc tacggcggca ttcacgcgat
    10681 ggccggcaac ccgaccgggt gcatgggact cgccgactat ctcggctggg atttcaaatc
    10741 aaaagccggt ctcccgattg tcaacgtgcc cggctgcccc gtccagcccg acaacttcat
    10801 ggagacggtg ctctacctgc tctaccaact ggcgggcctc gccccgatga ttcccttaga
    10861 cgatcagctt cgtccgacgt ggctctttgg caagaccgtc cacgaaggct gcgaccgcgc
    10921 cggctactac gagcaggccg acttcgccac cgagtatggc tcgccgaagt gcatcgtcaa
    10981 actcggctgc tgggggccgg tcgtcaactg caacgtgccg aagcgcggct ggatggccgg
    11041 catcggcggc tgcccgaacg tcggcggcat ctgcatcggc tgcacgatgc ccggcttccc
    11101 cgacaagttc atgccgttca tggacgagcc gccgggcgcg aaaatctccg cgggcgctat
    11161 cggcgtctac gggcgcatga tccgacgcct gcgcgccatc accaacacga cggtcaacaa
    11221 ggaaccgaag tggcggcaca cgggcgacaa actgacaacc ggctacaggc caaccaccta
    11281 cgactaagga gtgacacgag atgagtacga caacgatagt tggcacgaag gctccggcca
    11341 agccgcgcac gctggttgac atgaattggg acccgatcac gcgcatcgtc ggcagcctcg
    11401 gcatctacac caagatcgac ttcgccaaca aagaagtagc cgagtgctac tcgacctcct
    11461 cgatctttcg cggctactcg atcttcatga agggcaagga cccgcgcgac gcccacttca
    11521 tcacgagccg catctgcggc atctgcggcg acaatcacgc gacctgctcc aactacgcgc
    11581 agaacatggc cttcaacgtc cgcccgcccg cgctcgccga gtggatcgtc aacctcggcg
    11641 aggcggccga gtacatgttc gatcacaaca tctttcagga caatctggtc ggcgtggact
    11701 tctgcgaaca gatggtcagg gaaaccaacc ccagcgtctg ggagaaggcc aaaaacacgg
    11761 aggccccgca cgccgccgat cacggctatc gcaccatcgc cgacatcatg acggcgctca
    11821 atcccttcac cggcgagttc taccgcgaga cgctgcacgt cagccgctac acgcgcgaga
    11881 tgttttgcct gatggaaggc cgtcacgtgc acccctcgac gctttatccc ggcggcgtcg
    11941 gcaccgtgcc gaccgtgcaa ctcttcaccg actacatcgt ccgcctgatg cgctattgcg
    12001 agttcatgaa gaaagtcgtc ccgctccacg acgacctctt cgacttcttc tacgacgccc
    12061 tgcccggcta cgagaaggtc ggcgagcgcc gcatcctgct cggctgctgg ggctcattca
    12121 acgaccccaa cgtctgcgac taccgctacg agaacatgga cgagtggggc aacgcgatgt
    12181 tcgttacgcc gggcgtcatc gtggacggca agcacgtcac caccaacctc gttgacatca
    12241 acctcggcat ccgcatcctt ctggggagct cctactacga cgactggcaa acggccgaga
    12301 cgttcgtcaa aaccgacccg ctcggcaacc cggttgacca aaggcacccc tggaaccaga
    12361 ccacgacgcc gaggccgcag aagcgcgact tcgacaacaa ctacacgtgg gtcatgtcgc
    12421 cgcgctggta tcacgagggc gagcagggcg gcggcagcaa gtacctcgcg ctcgacacgg
    12481 gcggcggccc catcgcgcgt ctctggtcaa cggcgctcgc gggcaaggtg gacatcggct
    12541 acatcaaggc gaccggccac agcgtcaaga tttatctgcc gcagacggcc ctcaagcctg
    12601 aagtggagtt tgagtggaag attccgaagt ggtcgaacac catcgagcgc gaccgcgcgc
    12661 gcacttactt tcaggcttac gcggcggccg ccgccctcca cttcgccgag caggccctcg
    12721 ccgagttgca cgcgggacgc acgaagacgt gggaaccctt cacggtgccg gacgaggcca
    12781 tcggctgcgg cttccacgag gccgtgcgcg gcgtgctctc gcaccacgtg gtgatccgcg
    12841 acggcaagat cgccaactat cacccgtacc cgccgacgcc gtggaacgcc aacccgcgcg
    12901 acacctacgg cacgcccggc ccctacgagg atgcggtgca aaacaccccc ctcttcgagg
    12961 agaacggaca ggacaacttc aagggcatcg acatcatgcg taccgtgcgc agtttcgacc
    13021 cctgcctgcc ctgcggcgtc cacatgtatc tcggcaacgg caaagtcctg gagacgcgcc
    13081 actcgccgat gttcggcgcg atgaagtaaa cgagtagtga taagtgatga gtgataagtg
    13141 ataagacaaa aactgtcttt ctctttctca tcacttatca ctcatcactt atcactctat
    13201 ggaatctcaa caactaattc agcggattga agaactcgtc cgcaagatcg aggcgacggc
    13261 cgaccccgaa gcgcgctcta ccggcgtgga gcttctgcaa tcgctgatgg agcttaacgg
    13321 cgcggggctg gagcgcatga tggagatggc cgcggaaacg ggagcggcgg gccacgctct
    13381 catcgacggc tttgggcggg atgagttggt gaggggattg ctgctgctct acgggctgca
    13441 cccggtcgct cttgagacac gcgtggcgga ggcgctggtc aaagtacggc cctacctgca
    13501 atcgcacggc ggcaacgtgg aagtgctcgg cattgaagac ggcgtcgtgc gcctgcgtct
    13561 tcagggaagc tgcaaaagtt gtccctcgtc ggccatgacg ctgaagctcg ccatcgaaga
    13621 ggccatctac gaagccgcgc ccgatgtcac gtcgattgag gctgaaggag tgttggagcc
    13681 gcccgtcgcc tcgtcgggcc ttgtgccttt ggggagactg cgaagtgagg aagccgctcc
    13741 tccgcagcca aacggcaacg ggcatggatg gcaggaagtg agcggtctcg cggctctcgc
    13801 tcaaggggcc gtgcagacta tcgcggtgtc gggccgcgcg cttcttttct gccggctgga
    13861 cgaaaccttc tacgcttaca gcaacgactg tcccggatgc ggcgaagcat tgcaggaggc
    13921 ttaccttgag gcgacgagca tcgtctgccc cgcgtgccgg cagcgttacg actgcgtggg
    13981 cgccgggcgc ggactcgatc agccgaacct gcaacttgag cctttcccgc tcttgatgga
    14041 gcagggccgc gctaaagtgt ccctgcccca tctggcggcc agttgaatct tgccgggcct
    14101 gtccgggtcg cgggtgtata taccgtttac cgagacggcc atgtgaattg aggaggatcg
    14161 aacgatggac gaaatggaca aggaacacga ttccaagagc gaatcgcccg cgcacgattg
    14221 gggcacgggt aagggcgagg ataaatcttc gaccgaagga aaagaagccg gtcgccacga
    14281 tacgggggcg acccacgcgg atcgccccgc cggcacttcg acggcgaggg attccacgag
    14341 catcaatcct gagaacatgg acccgattga cccgaacagc ccgaacatgc caccggcata
    14401 agacgactcg cgggttggga cgagcgttgg acgccttgaa gcacgcgtca catttttccc
    14461 cgtccgcatt ctgagatggt atgagtgaga gttcgagaga tcgtggggag cagtcgccac
    14521 tcgccgcgct ccggcggttc gtgcgcccgc gcgcggcgaa cgtggaacgg tgcgagatgt
    14581 gtagcgccgc actggcggcc gagcatcaac acctcgtcga gccttcaaac aggcagatgg
    14641 tctgcgcctg tgagccttgc gcgattcttt tcagcggcca gacgggaacg aaatacaagc
    14701 gcgtcccgcg ccgcatccgc gtgctaccgg actttcgtct gacggacgga cagtgggaca
    14761 gtttgatgat cccgattcaa ctggccttct tcttccacag cacgccggac ggaaaagttt
    14821 tggcgctcta tccgagcccg gccgggccga ccgagtcgct actggcgctg gattcatgga
    14881 acgaagtcgt ccgagaaaat cccgtcctgc gggagatgga gccggacgtg gaggcgctgc
    14941 tcgttaatcg cgtggggggc gggggtgaat actatctcgt gccgattgac gagtgctaca
    15001 agctggtcgg cctcatccgc gcgcactggc gcggtctctc cggcggcacg gaggtctggc
    15061 gtgagatcaa acaattctac gcgggactga atgagagggc gtgaaaaggc aaaagtaaaa
    15121 aggcataagg caaaagtgag gacaaaagca tttcaataat caaaagtggt ttcagcctta
    15181 cttttgcctt tttactttta cctttttact ttaattgtgc ctgacttaaa ctttcaagtg
    15241 gaaggagctg aagccgtcgc ttatgcggcg gctccgcttc tttcgttcaa actacgagtc
    15301 accaacgccg aaggcgacga acccattcac accgtcgcgc tgcgctgcca gattcaaatc
    15361 gagacgacgc gccgtcggta taacgagcgg gagcaggagc gtctcctcga ccttttcggc
    15421 gagccggagc gctgggggca aaccctccgc acaatgttgt ggacgcacgc gagcatggtc
    15481 gtcacgccct ttcagggaag cacgctcgtc gagctgcccg tgccatgcac gttcgacttc
    15541 aacgtcgccg ccacgaagta ttttgccggt cttgaagacg cggaagtccc gctcgccctg
    15601 ctcttcagcg gcaccgtctt ctatgaggcg gcggacgggg ccttacaggt ttcgcagatt
    15661 tcgtgggaga aggaggctaa gtatcaattg cccgtgtcgg tgtggcaaca gatgatgaac
    15721 cactactatc cgaacagcgc gtggctctcc ctgcgccggg acgttttcga ccgtctgtcg
    15781 cggtacaaga tgcggcgcgg gattccgaca tgggagcaag cgttggagag tctcctgccg
    15841 gcagtcgagg agacgggcga ggcgcgtgag attgaggaga agatacaggc gtgaatctcg
    15901 aacgggtgga aaagattgct aacgccgtgc tctacgaggg gtacatcctc tatccctatc
    15961 gctcatcgtc ggtcaagaat cagcagcgtt ggaacttcgg ggcgctctgc ccgcccgcct
    16021 acagtgaggc gcaagggggc acggaaagtt gtcagatgca gacggagtgt ctggtcgaag
    16081 gcgctgagca cgccgcgctc gacgtgaaag tgcgtttcct gcatctgctg acgcgtgagg
    16141 tgcgacgatt gacaaccccg ctgcccgagc tgccggaagg cgcggagcct gacttccaac
    16201 gggtcgcggc gatggaggtc ggcggccgga tttatcaaac gtggcaggaa gccgtcgagc
    16261 gcgaagtgga cttgccgggg atcaatctga gcaagctcga cgcggagggg cagcgacaga
    16321 ctttcacctt tccgtcaaag caggagacgg aacctttgcg cgagtcggat ggacaaacgg
    16381 tcggcgtgct cgtccgcaaa cagcagttga ttgaaggcgc aattgaggtg gtggccgagc
    16441 aggcgcgcga gggtttgttc aaagtcaccg tgcgtatcct gaacctgacg ccgctggagg
    16501 atgcggcgac gaggagccgc gacgaggcgc tgatgcgctc gctcgtctcg acccacacca
    16561 tcttgggcgt gcgcgagggc ggcggcgagt tcgtctcgtt gctcgaaccg cctgaagcct
    16621 ttcgagagat tgccgcctcc tgccgcaaca cggggacgtg gccggtcatg gtcggtgaag
    16681 agggagcacg tgattgtatg ctctcctcgc cgatcatcct ctacgactat ccgcagatcg
    16741 cgcccgagag cgcgggcgac ctcttcgacg ggacggagat cgacgagatt ctgactcttc
    16801 gcattatgac cctgacggat gaagagaaac gggagatgcg cggcgtggac gagcgtgcgc
    16861 gccggatact tgagaggacc gaacagcttc ccgccgagca gatgatgaaa ctccacggcg
    16921 ggataaaaaa agttcaaagt tcaaagttca aggttcaaag tgaagagcct gtctccgact
    16981 ttgaaccttg aactttgaac tttgaactta ttgagttgag cgatgaacga atgggaattt
    17041 cagctcctcg aagagaggac tctcgtcgag caggttgagg tgggcggcgc acacatcaaa
    17101 acgggcgacc gcgtccggct gcggcctcgc gcgggcggcg acattttcga cctcgcgctc
    17161 gccgggcaaa tcgccatcgt cgaatctatc gagcaggact acgaggacag ggtgcatctg
    17221 gccgtcgtgc tggaggacga tccgggtcag gatttgggac ggctgcggca acccggccac
    17281 cgcttctttt tctcccccga agaggtcgag ccgctgccag cgcccggcgg cgaggatgcg
    17341 aaaagcggat gacccaacca cgcatactca tcgccggcat tggcaacatc ttcctcggcg
    17401 acgacgcttt tggctcggaa gtggcaaggc gtctggccgt gcgcgagttg ccggaggagg
    17461 tgcgtgtcgt tgacttcggg atacgcggct tcgatctcac ctacgcgttg ctcgacggct
    17521 acgaagtaac catcttcgtt gacgcgactc cgcgcggaga agcgcccggc acgctctaca
    17581 ccatcgagcc gaacttgagc gagctggatg acctcgacgc cgaaagcatg atggtcgagc
    17641 cgcacggcat gaatccgttg aaggtgctcg gcatggtcaa gtcgatgggc ggcgaattca
    17701 agcgtatctt tctggtcggg tgcgagccgg cgccgctttg ctcggaagac gggcacatgg
    17761 ggttgagtga gccggtaacg gcggccgtgg acgaagcggt gaaggtggtc gagtcgctcg
    17821 tccgcgagat tctcgaagag agaggtcaga aggcgacggc agtttgagtg atattcagaa
    17881 gtaaagggag atcatctgtc atgaagcata atcaatcgtt ggagcgaaga gtcaggaggt
    17941 cagccggtat gggtctctgg tcaattgttg gaagcgcggc gttggtactg gccgcggccg
    18001 cggttgtcgt gagcctgccc gacatcaaac gctatatcaa gatcagcagg atgtaacacg
    18061 cgcccgtcag cagttaagaa gcgaacgagg ggcgcaggcc gtgaagttca tctatggaaa
    18121 gacgtggcgg caggtgtcgt cggcgggctg gccgcgtctg ccgtcatgta tggagtctca
    18181 agccctccag aacttatgag catgaatgag tccagcgttg ttcgctatcg gatagacgcc
    18241 ggccggagca ggttcacggt gcagggggtg gccgagggga tgctctccat gttcggccac
    18301 aatcccgtga tcgccatctg cggcttcggc ggcgacgtgc ggtgcgaccc cggcacgctc
    18361 gaagccgcgt cgctgctgat gctggtgcag accgactcgc tcgcggtcgt caacaaagtg
    18421 agtggtaagg ataaacggga gatggagcgc gggatgcgtg aagacgtgct cgaaatcgcc
    18481 cgttaccccg aggttgtttt catgagcacg aaggtctcgg cgaaccgcac ggctgaaggc
    18541 cagtaccaga cgaggatcag cggaaatctg tcactccacg gcgtgacgcg cgaacaccag
    18601 atcaatgcac aggtgacggt caacggcagc agcctgcgcg cacagggcga gtccgcgttg
    18661 cgacaaagcg attacaacat caagcccgtc tccgccgtcg gcggcacgct caagatgaag
    18721 gacgagctga aactctcgtt cgacatcatt gcaagtgagc agtgagcagt gagcagtaag
    18781 cagtcaaact gctttttact gctcactgct cactgctcac tgctcactta atcatgcacg
    18841 agctttccat agccatgagt atggtggaga tggcggagga agaggctgcg cgccggggtg
    18901 gcgttaaagt ttttgcgatc cacctcaagc tgggccaact ttcaggcgtg gtcaaagacg
    18961 ccctgctctt ctcctatgag atagtctgtc agggcaccac tctggaaggc tcgcaactca
    19021 tcatcgaaga aattccggtg gtggtctatt gcccgacctg ccgggccgaa acgtcgctcg
    19081 cttcgcttca gcgattttgt tgtgcggctt gcggcactct gacatcggag gtcgtgcggg
    19141 gaaaagaact cgaagtcgtc gccatggaga ttcaatgatg agcagccaac cgcgcctcgt
    19201 cgaagtcagg caaaacgtgc tgaagcagaa cgatctgctc gcgcgcgagc tgcggagacg
    19261 ttttcaggaa gcaggtgttt gcgtcgtcag tctcgtctca agtcccggct ccggcaagac
    19321 ggcctttctg gaaaaggcgc tcacgctgct ccagcgttca aagttgcgtg tcgcggccct
    19381 cgtcggagac ctcgcgacgg agaacgacgc ggcgcggctc gcccgcagcg gcgcgcccgt
    19441 cagacagatc acgaccggca cgatttgtca tctcgatgca cagatggtcg agcgcgctct
    19501 cgaagaatgg aacctcgaag aactggatgt gctgttcatt gaaaatgtcg gcaacctcgt
    19561 ctgcccgtcg agttatgatt taggcgaaga cctccgtctg gttctcctct ccgtgaccga
    19621 aggcgaggat aagcctctca agtatccgac gattttcaac acggcggacg tggccgttat
    19681 caccaaaata gatttggccg aggccgtcga gtttgaccgg gccgcggcgc accgcaacat
    19741 ccagtccgtc cgccccggaa tgaaggtctt cgaggtttcg gcgaagacag gcgaggggat
    19801 ggatgagttg ttgccagtgc tctacagcgc ccgttccgcg gagtcggaga aagttctggc
    19861 gggctgagtc cttcagtacg atgagacgtt tctcatgcgc gttgccgaac acatttcgcg
    19921 aactacccgt gtgcaaatac tggtgcgcgg catcgtgcag ggcgtagggt ttcgccctta
    19981 tgtctactcg cttgccgcgc ggggcgcgct tcgaggccgg gtgctgaaca acacgacggg
    20041 cgtgctgatt gacgttgaag gcgaagatca ccgcatcgaa gagttcatca atgagattac
    20101 tttgaacccg cccccgctct ctcaaatcga atcgatccag cgccacgacg atctggcttc
    20161 ggcgaattat caagacttcc gcattgtcga gagtgcggcg gagggagtga agctcgttcc
    20221 cgtctcggct gacgtggcca cctgcgcgga ttgcctgcgc gaacttttcg attcgcaaga
    20281 ccgtcgccac cgctacccct tcatcaactg caccaactgc ggcccgcgct tcaccatcgt
    20341 cgaagccgtc ccctacgacc gcgcgcagac cacgatgcgc gacttcgaga tgtgcgcgcc
    20401 gtgccgcgcc gagtatgaga acccgctcga ccgccgcttc cacgccgagc cgacggcctg
    20461 cgcgtcttgc ggcccgaggc tgcgtctgac ggacgcgtgc gctcgcgcgc ctgagttcga
    20521 tgaggccgac ggcttagaca tcattggccg cgtgcgccgc ctgctctctt cggggaagat
    20581 cgtcgccatc aaaggcatcg gcggctacca cctcgcctgc gacgcgctcg acgcgggggc
    20641 ggtcgagcgt ctgcgacaga ggaagtacag ggaggacaag ccgttcgcgc tgatgggcgc
    20701 ttcggtcgat gtcgtcagag agtattgccg cgtctccgaa cttgaggaac gtttgctact
    20761 gtcggcacgc cggcccatcg tcctcctcga aaagagggcg ggcgctgtcc tccccaaagc
    20821 ggtcgcgccg cgcgtgaaca cgctcggctt catgctgccc tacgcgcccc tgcaccacct
    20881 gcttctcgaa gacctcgacc gcccactcgt gttgacgagc gccaacgtct ccgacgagcc
    20941 aatctgttat gaagacgcgg aggcgcgcga gcgtctgggc gcgatagccg actactttct
    21001 gctccacgac cggcggattc acatgcgcgc cgacgactcc gtggcgcgcg tgcacgcggg
    21061 gcgcgagatg atcctgcgcc gcgcgcgcgg ctacgcgccc gcgcccgtcc gcgtggcgtt
    21121 caggttcgag cgacagattc tcgcctgcgg cgcggagctg aagaacacct tctgcctcgc
    21181 gcgtgacaac tacgccttca tcagtcatca cgtcggcgac ctggaaaatc tggaaacgct
    21241 gcgctccttc gagcggggcg tcgaacacta ccggcggctc tttcacctca accccgacgt
    21301 ggtcgcctac gacctgcacc ccgaatacct ctcgacgaag tacgcgctcg cgctcgatga
    21361 aatcccggac aaaatcggcg tccagcatca tcacgcgcac gtcgcgagct gcatggccga
    21421 caaccggata gagggcgagg tgatcggcgt ggcgatggac ggtctcggct tcggcacgga
    21481 cggacgcctc tggggcggcg agttcttcgt cgcggacttt tgcgatgcgg agcgcgtcgc
    21541 ccatctcgac tacgtgccga tgcccggcgg cgcgaaggcc gtccgcgagc cgtggcgcat
    21601 ggccgccgtc tacctgcatc gcgcgttcgg cgacgagttt ctaaacctcg acataccctt
    21661 cgttcaaaat ctggaccggc gcgcgtggac gacgctgcga cggatggcgg ccacggccac
    21721 gaacagccca gagacttcga gcatgggcag actcttcgac gccgtggcgg gcctgttgag
    21781 tttgaggggc gcggtgaact acgaggggca ggcggcgatt gagttggaga cgattgcgga
    21841 gcgcgcgtgt agacaagaag gctacgcgtt cgagcttgag gagggcggca gggtcatcaa
    21901 ggccgaagcg gtcgtcaggc gcgcggtcga agacttgctt gaagggcgcc gcccgcaaga
    21961 ggtctccgcg aagtttcatc tcggggtggc cgcccttatc gcggcggtcg cccgacgggt
    22021 gagggacgag cggaagctgg aaagagttgt gctttcgggc ggcgtctttc agaatacatt
    22081 tctcctagaa gccgcctgcc ggatgcttaa gggcgacggt ttcgaggttt tcacacacag
    22141 ccgcgtgccc ccgaacgacg gcggcatcag cttcggccag gcggccattg ccgacgcgcg
    22201 tcttaaagca gggagggttt aatcgatgtg tttagcgata cccggaaaga tcgtcgagat
    22261 aattgacgag gaaaactcaa tcgccaaggt tgacgtgggc ggagtccgcc gcaacatcaa
    22321 cacggggatg ctcactcccg acgaggcgcg cgtcggcaac tacgtcctga tccacgtcgg
    22381 cttcgcgatg agcaaggttg acgagcacga ggcgatggag acgttacgca ttctggagga
    22441 gatcggccag ttcgatgagg agttcgacca gttcaaaact tctctggaat aaattgtagg
    22501 ggcagggctt gtccctgccc gcagggattc aatgtcattc aacttgagtt catgggcgcg
    22561 aacgggcagg gacaagccct gcccctacag ccatcttggc agaggtcatt aatgtctgag
    22621 tcggcaaaag atttctcgcg cgccttctac cctttcctgc acgcggacga gaagaagccg
    22681 caggagttga tggaagaatt gcgcttctcg ctgttggaga aggcgcgcga aagcgtggag
    22741 atcaagagcc gtttcttcga ggagaacaag gacaccatcc tcgccgcgtc gctggcgatg
    22801 gcgaaggctt ttcaccacgg gcgcaagctg ctcgtctgcg gcaacggagg ctccgccacc
    22861 gacgcgcagc acgtcaccgt cgagttcatg caccccgtca tcgtcggccg caaggcgctg
    22921 cccgccatct gtctgacgag cgacatggcg atggtcacgg ccgtggccaa cgatgtgggt
    22981 ttcgacgacg tgtttacccg gcagatcatc gcgctcgggg ccgagggcga catcctgctc
    23041 ggcatttcga cgagcggcaa ctccgagaac ctgaggcacg ccttcgccac cgcgcgcgcg
    23101 cgacggcttg tgaccgtcgg cttcgccggg ggcgatggcg gacggatgtg cgagctgggc
    23161 gcaaagggcc tgctggattt ctgcctcacc gtgcccactt cgagtatcca tcggattcag
    23221 gagacgcacg tcgccctcta tcacatcatg tgggacatgg tgcatacatt catgcagcac
    23281 cgctcgcttg tagaggagca agcggctgaa taattgtagg ggccgcggac ttgtccgcgg
    23341 cccggagttg ccgaagcaat taacatggat gggcaggatg ggtaggataa agaaaggact
    23401 gatttcagat ttgagatttg agatttcaga ttttctgatc ctgctcatcc tgtccatcca
    23461 tgttaattct tctttcgctg gaacgggcag cggacaagtc cgcggcctct accgcaaatc
    23521 ttgggggcat tatgaaattc gtagacgaat accggcgggg cgacatcgcc gagagactcg
    23581 cggggcaggt ggcccaactg acggagcgcc cgctcaagct gatggaagtc tgcggcggcc
    23641 acacgcacac gatcttcaag tatggcatcg aagacttgct gcccgagaac atcacgatga
    23701 ttcacgggcc gggctgccca gtctgcgtca tccccctggg gcgcgttgac gacgccatct
    23761 cgatagccca acagcccggc gtgatcttca cgaccttcgg cgacgccatg cgcgtgcccg
    23821 gctcgcgcac cagcctgctc gacgcgaagg cgtcgggcgc ggacgtgcgg atggtctact
    23881 cgccgctcga cgccttgaag attgccaaga agaatcctga gcgcgacgtg gttttcctcg
    23941 cgctcggctt tgagaccaca gcgccctcga ccgccctgac cgtccttcag gcggcgaaag
    24001 acggcgtgga aaacttttcg gtcttttgta accacatcac gattgtgccc gcgctcaagg
    24061 cgctcctcga ttcgcccgat ctgcaactcg acggcttcct cggccccggc cacgtcagca
    24121 ccgtcatcgg cacgcggccc tacgacttcg tcccccaaga gtacggcaag ccggtcgtcg
    24181 tcgcgggctt cgagccgctc gacatcttgc agtccgtcta catgctcgtc aggcaggtcg
    24241 tcgaggggcg cgctgaggtc gagaaccagt acggccgctg cgtccatcgc gacggcaacc
    24301 ggaaggctct ggaagttctc ttcgaggtct tcgagccgcg cgacttcttc gagtggcggg
    24361 gcctgggctc catcgcgcac agcgggatga agctcagccg caagtatgcg gccttcgacg
    24421 cggaactcag gttcagcgtg ccgggcgtgg gcgttgccga tccgaaggct tgccagtgtg
    24481 gcgagattct caaaggcgtg aagcggccgt gggagtgcaa ggtcttcggg acggcctgca
    24541 cgccggagac gcccatcggc tcctgcatgg tctcctcgga gggcgcgtgc gccgcttact
    24601 acaacttcgg ccggctgtcg aagatcgccg agcgctccgc gccgaacggc gaaagcaaac
    24661 gggccgtcga ggcgggcgtg acggtttagc agatgacgag cgagttcaac ccatcgccgc
    24721 tcttcgagcg cgtcgagcga gcgcggcgtc ggaagcgcaa atttaaggac gagcagatca
    24781 cgctcgcgca tggcagcggc ggacgcgcca tgcacgaact gatcgaaggt ctctttcttg
    24841 aatacctccg caaccccgtg ctcgaagccc tcgaagatca ggcggtcttc gaggtggggg
    24901 acgggcgcgg ccccgcgcgc ctcgccttca cgaccgactc ttatgtggtc acgccgatct
    24961 tctttcccgg cggcgacatc ggccgcctcg ccgtctgcgg cacggtcaac gacttgtgcg
    25021 tgagcggcgc gcgccccctg tatctctccg ccggtttcat cctcgaagaa ggttttgcca
    25081 tcgaagacct caagcgcgtg ctcgtgtcca tgcgggcggc ggccgaggag gcgggcgtcg
    25141 ccgtcgtgac gggcgacacg aaggtggttc agaaggggag cgctgacaag atatttatca
    25201 acaccgcggg gctgggcgtg atcgagtcgc ccgtgcaaat ctccgccgcg cgcgcgcagg
    25261 tcggagactg cgtgatttta agcggcgcca tcggagacca cggcacgacc attatgatcg
    25321 cgcgcggcga gcttgagctg gagaccgaca tcgaaagcga caccgcgccg ctgaactctc
    25381 tggtcaggga gatgctcgac gaggcggccg gcgccgggtc gctcgaaggg attcatgcgc
    25441 tgcgcgaccc gacgcgcggc ggcgtggcga cgaccctgaa tgagatcgcg ctggcgtccg
    25501 aagtatgcat cgaaattcgc gaagacctca tccccgtgcg cgaggaggta catggcgcgt
    25561 gtgagattct gggcctcgac ccgctctacg tcgccaacga gggaaagctc gtcgcggtcg
    25621 tcgccgccga catcgccgag cggctggtcg cgcggatgaa gcaaaacacc tacggccgag
    25681 cggcctgcat catcggcgag gtgaaggccg agccgaaggg catcgtggcc atgcgtaccg
    25741 gcttcggcgg cacgcgcatc gtggacatgc tcgtcggcga gcagttgccg cggatatgtt
    25801 gatcgaggag ctgttagcta ttagctgtca gcccacggcc agcgccgtcg gatgtgctgc
    25861 cgctgataac taaacgccac ggctcgccat cttttgctaa cagctaacag ctctatttat
    25921 atgacttcgc tctccaccgt gtcggtgctc gctttcggct tcgtgctggg actcaggcac
    25981 gcggtcgaag ccgaccatct ggcggccatc tcgaccatcg tcagcgagcg caaaagtatt
    26041 ctgagttcgt cgctggtggg cggtctgtgg ggagtgggac acactatctc gctggtcgtc
    26101 gcgggcgtcg ccgtcattct gctgggggtc gagattggcg cgcggacggc gctggcgctg
    26161 gagttctgtg tcggcctcat gctcgtcgcg ctcggcgcga acgccctgcg gaagctcgcc
    26221 cgcggaggcc gcgtccacct ccacgtccac cagcacggcg gacgcgcgca catccaccca
    26281 cacgtccacg gcaactcgcc cgaagaagcc ccgcacacgc accacggcct gcgcctcgac
    26341 acgcgcacgc tcgtggtggg gatgattcac gggctggcgg gcagcgccgc cctaatgctc
    26401 ctgattcttt caaccatcga gtcggcaacc gttgggttcc tctacatcgt cgtcttcggc
    26461 gtcggctcga taggcgggat gatgttgatg agcgcgctca ttggcctccc gctctacttc
    26521 acggccgcgc gcttcaaccg ggccaacctc gcgctccgcg gggtggcggg agtcttcagc
    26581 ctgagctttg gtttgctcat ggtctatcag atcggcttcg tcggcggact gtttcgttaa
    26641 gcggattgaa tccgccgccc tgccggggag cggttcgttt cgcggccggg aagaatcatg
    26701 aaggggcgaa aggttccagc gtgggttagc gtgccgctcg tcgtcggcgc gttcgggctg
    26761 ctcgcgtggc tggagcgacg acgcccgctg cggcgctcgg tcgaacccaa gctccggcgc
    26821 gaggcgcgta atctggccgt ggccgccgtc ggcgcggcgg cgctccgggt tgcggaacag
    26881 cccgtcgtcg ggccgctctc cgccctcgtg gaacggcggc ggtggggtct gctcaagctc
    26941 tggaagctgc cggtctggct ggagacggcc ctcgccgtgc tcctgctgga ctacacgctc
    27001 tacgtgtggc acgtcctgac gcacaagcaa ccgtggctgt ggcgctttca cctcgtgcat
    27061 cacctcgatc tcgacctcga cgcctcgacc gccctgcggt ttcacttcgc ggagcttatg
    27121 gtctccgtcc cgtggcgcgc ggctcaagtc ctgctcatcg gcgtctcccc gctatcgctc
    27181 tcggtctggc aaaccttcct gttcctctcg atcctgttcc atcactcaaa cgtgaggctg
    27241 ccggtcgaag tcgagcgccg gctgaactca ctcgtcgtca cgccgcgaat gcacggcatc
    27301 catcactcac tcgtgaagga ggagacgaac tcgaactggt cgagcgggct gaccctgtgg
    27361 gacagactgc acgggacgct ccgccagaac gtgccgcaag acgcgatcat catcggcgta
    27421 cccgcctacc gcgaccccga cgaagtcacc ctcccgaagc ttctccgaat gccgttcggc
    27481 gaggagcgcc cgacgtgggc gttgccggaa ggcgcgacac cacgccgcgt cgccgcgcct
    27541 gtcgcggagg atcacttgct ggcttgagtc gtagacaaaa gaccatgaaa cagagtatcg
    27601 cgcatattgc attggtggtg gacgactatg acgaggccat caaattttat acggagaaat
    27661 taaatttcac tctcgtcgaa gacaccgctc agagcgaaac aaagcgttgg gttttagtcg
    27721 cgcctaaggg ggcggaagaa tgtagcttgt tgctggcgaa gggggtcggc gaagaacaaa
    27781 gaagccgcat cggaaaccaa acgggcgggc gagttttcct attcttaaaa accgatgact
    27841 tctggagaga ttataaaagc atgctgtcgc aagacatcgt ttttgtcaga gaaccgaaga
    27901 acgaggaata cggaacggtc gccgtcttca aagatttata cggcaacctc tgggacctga
    27961 ttgagttcaa aaaatagaag tgagtgaagt gtctcattat cctgattagc atgccgaaat
    28021 ggataatcga accactaaac caaggcccga aggagagcgc cccccggccc tttcgatcat
    28081 catcccgacg ctcaacgaag cgcgctcaat cggtcaaacc ctcgacgcgg tcatgggcgc
    28141 tcagggcgcg gtcgaggtga tcgtcgttga cggcggaagc gttgacggga cggcggagat
    28201 cgcgcgcggg cgcggggcgc ggctcgtcac ctcggggcgc gggcgcggcg agcagatgca
    28261 cgcgggggcc tgcgccgcgc gcggaagcgc gctgtggttt ctgcacgcgg acacgcgagc
    28321 gtcagcaggt tgcgccgaag ccgtcgccgg ggcattgcgt gaccctgagg tggtcgccgg
    28381 caacttcagc gtgcgcttcg acggagacgg tcgcgcggcg cgtttcctca cgtg
//
LOCUS       DASVES010000475        20836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1450562, whole genome shotgun sequence.
ACCESSION   DASVES010000475 DASVES010000000
VERSION     DASVES010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450562"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..2666)
                     /locus_tag="VF240_19875"
     CDS             complement(225..2666)
                     /locus_tag="VF240_19875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177528.1"
                     /translation="MDTILQDLRYGIRMLTKSPGFTAVAVLALALGIGANSAIFSVVN
                     AILLRPLPYKDPGELVLINHNYQKINLKASVSVPGYSHYRDNAQSFEGVGAVTGWNAN
                     LTGTGGEPERLQGMAVTHDLFPLLGAEAARGRTFTAEENQPGNNKVVVLSDALWQRRF
                     GSDAGIVGKDLTLNGESHKVVGVMPPGFQFGRELGPLVEVWAPVGWTPQQLQMSNLTN
                     ESLAVLARLKPGVSIGQAQVEMDNIADNLRTQYSPGMTAADWGLLLTSFNETVVGDIR
                     PALLILLGAVALVLLIACANVANLMLARSAARQKEIAIRTAMGASRGRIVRQLLTESA
                     LLSVIGGGVGLLLGLWGVEFLVAINQDKIPRAHEIGLDLTVVLFTLGVSLVTGIVFGL
                     APAFQTSKIELHDTLKEGGRSGVGVMRRGVRGALVIAEVSLAVVLLVGAGLLIRSFYR
                     LQDVSPGFDPQNLLVMQVSLPGSKYPEPQQRDAFYQQLMEQVRAIPGVGSAGATAILP
                     MSGNNQSGSFRIEGREVPQGASLPHGDRWAATADYFKTLGVPLVRGRFFTERDLSDAQ
                     PVAIIDETMARKYWPDEDPMGKRISYEGGAANPRWREIVGIVGHVKHKGLEGESRVQY
                     YIPHPQLAQGSMFLAVRPSAGEATSLAGAVRGAVASIDRDLPVFRVTTMEQLVSDSLA
                     QRRFSMLLLGSFAALAMVLAVIGLYGVMSYTVAQRTHEIGIRMALGAQPGNITSMVVG
                     QGAKLAALGVVIGLTGAFLLTRVMSGLLFGVSATDPQTFAGVAVLLGLVALLACYIPA
                     RRATRVDPMVALRYE"
     gene            complement(2685..5129)
                     /locus_tag="VF240_19880"
     CDS             complement(2685..5129)
                     /locus_tag="VF240_19880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177529.1"
                     /translation="MMGTLWQDIRYGVRMLWKSPGFTLVAVVALALGIGANSAIFSVV
                     NAVLLRPLPYPEPERLVRVGGTSARAAEAIGTHSPADFFDWREQSTSFEAVAAYDGWS
                     PSLTGAGEPERINAARVSASFFSVLKSEPAVGRGFLPQEEQRGQHRVVVLGHGLWQRR
                     FGGAQNVVGQQIELNGEAFTVVGVAAREFESPEFTGLGATEPPELWAPFAPDLAQWTR
                     SSRSVDACLARLKPGVTVEQAQAEMATIAARLEAQYPETNKNAGVRLVSLHEQLVGGS
                     RRALLTFLVAVGFVLLIACANVANLSLARASARRREIAIRTALGASRLRVVRQMLTES
                     VMLSLMGGLLGLLFAWWANDLLLALGADSIPRIAGVGLDARVLVFTLAASLLTGVAFG
                     LVPAIQSTRLDLNEALKEGGRSQGQPAARRRARALLVVAETALSLVLLVGAGLLVRSF
                     VRLLAVDPGFDPRGAVTMNVFLPGARYPEDAQHAAFFDAVVERAAALPGVESAGATSN
                     LPVSGNYDRMPVYVEGQPAQLAGDAPDAERYMVSADYFRALGVPLVAGRAFDARDREG
                     APLVAVIGRSMSERFWPNEDPLGRRIKFGDVKNPWVEIVGVAGDVRHYGLEKEANLQI
                     YLHHLQTPSQVMTLVVRGPSGGDPEALARAVRGEVWAVDKNQPVYDVKPMERIVAESV
                     APRRFNTLVVGVFAAVALALAVIGLYGVMSYTVSQRTHEIGIRMALGAQGRDVLRMIV
                     GQGMWLTAAGMGAGLLGALLLTRAMSGLLYGVSASDPLTFAGVAVLLAAVAFVACFVP
                     ALRATKVDPMEALRHE"
     gene            complement(5216..5929)
                     /locus_tag="VF240_19885"
     CDS             complement(5216..5929)
                     /locus_tag="VF240_19885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177530.1"
                     /translation="MSTAPDNGKPLIHLEDVKKVFYTDEVETHALSGTYLDIQSGEFV
                     SITGPSGCGKSTLLSILGLLDSPTDGHYWLNSKDVSELSLSERAVIRNREVGFIFQSF
                     NLIGDLTVYENVELPLTYRGMSGAERKKRVGEALEKVNMAHRAKHLPSQLSGGQQQRV
                     AVARAIAGDPLILLADEPTGNLDSTNGEAVMALLRELHQVGSTICMVTHDPRYAKFAD
                     RAIYLFDGRVVSEEQVEAA"
     gene            complement(6036..7454)
                     /locus_tag="VF240_19890"
     CDS             complement(6036..7454)
                     /locus_tag="VF240_19890"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7177531.1"
                     /translation="MPSNDETRPGVADDKEDMNTKISPIRSEKGEGQDVPRKGQDQPR
                     KTGFTTPSNTMDVPRKKSAARKRRVKNIGLAVVAVAAIAGITFAVSRMKPAAPGVQRS
                     TVLIDTVKRGEMLRQVRGIGTLVPEEVRVVAASTQGRVERILVQPGEDVGAGTILIEL
                     ANPELQQQVVDVQYQLLSAEADLNNIRARLDNERMAQQAAAATVRAEYQQAKIIADTD
                     QTLAKEGLIPELNTRLSRVRAEELANRYAIEQRRLEGRKKSDEAQMGSQVARVNQLKA
                     ELGLRRQQAASLHVLAGTNGVLQQRLVEVGQEVTPGTVLARVVEPQNLKAELRIAETQ
                     TSGIAIGQPASIDTRNGVIPGRVSRIDPAAQGGTVAVDVQLEGALPAGARPDLSVDGT
                     IELERLENILYVARPTFGQANATVGIFKLDEGGSSATRVQVRLGRSSVNTIEIVEGLK
                     EGDQLILSDTSQWDAFNHIELK"
     gene            complement(7534..8217)
                     /locus_tag="VF240_19895"
     CDS             complement(7534..8217)
                     /locus_tag="VF240_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177532.1"
                     /translation="MIELRNVEKAFEQGNTKNFVLRRINMHVREGEFVSIMGPSGAGK
                     STLLGILGMFDSAWKGEYYFMDKPVHALNRKERNDLHNRHIGFVFQSYHLLDHLTVYE
                     NLELPLTYKDIKKGDRQSMVADTLDRFHIVGKKDLFPNQLSGGQQQLVGVARAVIASP
                     KLILADEPTGNLHSGQGREIMELFKGLNDAGATIIQVTHSETNASYGNRVINLVDGWM
                     ADEGQTSAA"
     gene            complement(8498..9064)
                     /locus_tag="VF240_19900"
     CDS             complement(8498..9064)
                     /locus_tag="VF240_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177533.1"
                     /translation="MATKKASRKSGSGKKSAKKKSAAKKSSGGKSGGARKGGAGKKGG
                     AKKSGAKKSAAKKSGAKKSAAKKSGAKKSGAKKGGSKKSGASKKSGAKKSAKKRGGGR
                     KGGKKPGLIKRAAKVAGEMIAGAASGAVKGAVVGAVEPLEEAVGVERTEVETSDATGG
                     AGSKGGGTQGGESQGSGGKSGSGGGSVG"
     gene            9333..11258
                     /locus_tag="VF240_19905"
     CDS             9333..11258
                     /locus_tag="VF240_19905"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HEX7177534.1"
                     /translation="MTRKKTLQQRWEFDLRSSPEALWPYVADTNRFNSDTGLPAVAVA
                     DDEAGDEATTGAPRNARRRLRVKVSVLEMLYEEEPFEWVRPHGFGVVRRYAGGPIAEM
                     RVRVELRPREGGGTHLVYDTEFTPRASVVRLALPLQLKLMRRKFERAFRRYDRLAAER
                     ATAPQTAATPNAAAPLSERTQLTPTGRERLRQIGRALVAEGKNAELVRRLSELVERGD
                     DLALARVRPYALADLWTADRREVLELCLAATRAGLLDFRWELLCPLCRGSADSPASLG
                     DITAGQHCATCGIDFEVNFDRSVELTFRPNPAVRKVEGAAFCVAGPATTPHVVVQQLL
                     RPGESRTVRPRLEEGRYRLRALGLSGAQQVRVAGAGAGGVVSLGPTEAARWPSDERLI
                     GVEPALRIENKDAVERLFILERTSWSDQAATAAEVTALQVFRDLFAEEALRPGEQISV
                     GSLTLLFTDLRGSTQLYRQIGDAVAFGAVMTHFDVLRESIAAEGGAIIKTLGDAVMGA
                     FTRPAPALRAAMRAQAMLAAPPAGVRPLALKVGIHTGPCIAVTLNGRLDYFGSNVNIA
                     ARLEPLSSGSDCIISRPVRDDPKIAALLSGQASGLVAEPLRTTLKGFDAEHFDLWRVT
                     RKEISPPETETPTKRGI"
     gene            complement(11312..13276)
                     /locus_tag="VF240_19910"
     CDS             complement(11312..13276)
                     /locus_tag="VF240_19910"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7177535.1"
                     /translation="MRLEDELTVVSADVLTGQVLDGKYELQRRLGEGGMGAVYRARRV
                     HIGDEVAVKILHPKFVADASAVERFRREARAAAQLHHHNVVTIHDYGETQTGGQPLAY
                     IVMELVAGVSLRELLKREGRLDLTRAVSLMRDVCAGVASAHRRHIVHRDIKPDNIIVL
                     APDDDRERETVKVVDFGIAKLRDASADHTLTAAGAVVGTPHYMSPEQCLGEPLDSRAD
                     VYSLGALFYEMIAGAPPFTAATPTGVISKHLSEPPPRVPADARIPASIEQTVHRALAK
                     DANARQTDASEFSRELSTAAAAGAATTALGPEANAAHPHAGAPPQPTTLPAAASTGRQ
                     PPAVHTAGPAPHVPHAAPQPPPRRKSRAPFWVGLMLLCGAAIAVAGFGFYMFLNANRN
                     TRPNRTAQHNTSANTNTNTNTNTNTNSNTNAQLAAFQRAEAKVLSNMRLVETELAGLD
                     NAALRLLLNTVYARHGRAFTPVDLRAHFRTRPWYKQSGDYTDERLTEADRANIRLLVG
                     AGAGPAPTPPPDQKEMRREVVDALDGWVSSTEDRDLDEHMSFYADTLEGYYNSRGVAR
                     TNVRTDRARAFDRYQKINVDIDNISIALDETGAGAQVTLDKKWKFEGERCSKGKVQQQ
                     LRLARIDGRWRITSERDLKVYPEESDTNCD"
     gene            complement(13543..14712)
                     /locus_tag="VF240_19915"
     CDS             complement(13543..14712)
                     /locus_tag="VF240_19915"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HEX7177536.1"
                     /translation="MLKRHLLRHAALLFALLVLAPAAAGATDEEAVKKWESFDFAKQL
                     VTREQLKDMPLEDLKLLRGLVFGRHGRVFKDWDIGIYLKERPWYKPDPNFSNSSLNDT
                     ERRNLDLIREMEARLHEVIEPGDMRWWQARAFNAKQLGEHNGAEWRILRAEIEAVHGK
                     RFDDQPWLQQYFEDRYWYQANAGYDPKLLTDIERRNMTTIDAAQRKQRNVALSPGDME
                     HFQKTPVSEAMLDGLSLHELRLLRNEVYARRGRRFRTEWLSQHFYSQPWYEPEQSEEF
                     KEPELSDIERKNIELIVGYENRIRGELSTKPVSTKLLDGLFLEDARKLRHEILARRGK
                     VFKDRWLQKYFESLGWYKPDPRFKEAALTTVEKKNVSAILAYEKKATSVLDAIEG"
     gene            complement(14853..15644)
                     /locus_tag="VF240_19920"
     CDS             complement(14853..15644)
                     /locus_tag="VF240_19920"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7177537.1"
                     /translation="MRLDANALALLLLLAWPLGALAVAQERKAEPKAVASPATVAAVK
                     ARAARTIIAIDPGHPSEVASGRGEQNGTNETRVAWEVALKLKTQLETEGYEVVLTKSA
                     EDELVRNRDRATVANRARAALMVRLHCDASIERGFAVYYPDRPGRTKDGAVGPSARVV
                     EASARAARAIHAGMSEVLEGQLDDNGVRTDYQTKVGREQGGALTGSIFSDVPVVTVEM
                     VVLSDAGDAEFIKSEAGQQKMAEAIAGGIARFVGPRVQAAPRGKN"
     gene            15901..16371
                     /locus_tag="VF240_19925"
     CDS             15901..16371
                     /locus_tag="VF240_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX7177538.1"
                     /translation="MRTDIIERRLEIENWIASRRPKAFMCRELQCKPSTLNSYLQKMG
                     LNYAGNMGGQGYKQCPTRKGARIYLRKGTLISSYKLKRKLLEDGLKKRRCEQCELTEW
                     LSQPIPIELHHINGDRFDNRFGNLKLLCPNCHALTDNHAGKGSNRIKVYSNNCN"
     gene            16377..16458
                     /locus_tag="VF240_19930"
     tRNA            16377..16458
                     /locus_tag="VF240_19930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16411..16413,aa:Leu,seq:tag)
     gene            16565..17914
                     /gene="tig"
                     /locus_tag="VF240_19935"
     CDS             16565..17914
                     /gene="tig"
                     /locus_tag="VF240_19935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7177539.1"
                     /translation="MKTEIVDVSATRKEFKIEIDAAEVRAEYDRVSGEYARKATVPGF
                     RKGHAPASVVRTRYKKEISGEVLQKLVPEAVNRALEESGLRVLGQPDVHLAPESIERL
                     GEAPVSLHAHVEVLPDVELGEYKGLSAARRTRPVTDEMVEQTLAGLRESSASLQPVEE
                     RGAESGDTVTVNFHGRFVEPAGAEDITADDVDVVLGGEGVQEDFNANLMGARADEEKT
                     FTVKYPEDFSSKGLAGKTVDYTAKVVAVSRKELPELDDEWAKSLGEEIDSVESLRRRV
                     RENLEGRAKFEAEGRVRDEVVGRLVDAHKFELPESLVEYQTNQMLQTAVRDMIRHGLD
                     PRAEQFDWEALRGGARERAEEELRASMLLETIAERENIDVTDEEVEEEIKMIAEASRQ
                     TVEQVRAALTKQGGATSIADSLRNRKAVDLLVEKANVSEEEWREEKQESEVTSQESE"
     gene            17975..18562
                     /gene="clpP"
                     /locus_tag="VF240_19940"
     CDS             17975..18562
                     /gene="clpP"
                     /locus_tag="VF240_19940"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX7177540.1"
                     /translation="MALVPMVVEQTSRGERAFDIFSRLLKDNIIFIGTPIDDQIANLI
                     VAQLLFLEAEDPEKDINLYINSPGGSITAGMAVYDTMQFIRPDVTTICVGQCASMAAL
                     LLAAGAHGKRFALPNSRILIHQPSGGAQGQASDIRIMAEEILRMRELTSRIIAKHTGQ
                     TYEVIERDVERDRIMSAAQAKEYGLIDEVIAHREA"
     gene            18804..20045
                     /gene="clpX"
                     /locus_tag="VF240_19945"
     CDS             18804..20045
                     /gene="clpX"
                     /locus_tag="VF240_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX7177541.1"
                     /translation="MVRRTDDTLRCSFCGKSQNEVKKLIAGPTVYICNECIDICNEII
                     TDDQQAEAVASRPPLPKPAEIKTFLDEYVIGQDETKKRLAVAVYQHYKRIDIARRRSD
                     VEIQKSNILLIGPTGTGKTLLAQTLARILAVPFTIVDATTLTEAGYVGEDVENIILKL
                     LQAANGDVERAQQGIIYIDEVDKICRKDENPSITRDVSGEGVQQALLKILEGTVANVP
                     PQGGRKHPHQEFFPIDTTNILFICGGAFVGLEKVIERRLRNKSMGFQAEVKSSGARHA
                     EALGFVQPEDLIRFGLIPEFVGRLPVCGVLHELDEAALIRILTEPKNALIKQYQKKFE
                     YDNVKLRFTEDALKAIARQAVERKVGARGLMMIMEEMLLEAMYTLPSQKRIKEFVITS
                     EMVERRRAVPGEVLTGVDEAA"
     gene            20241..>20836
                     /locus_tag="VF240_19950"
     CDS             20241..>20836
                     /locus_tag="VF240_19950"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7177542.1"
                     /translation="MEEYSDRTPPDIARLPMVPIRDVVIFPFTKVAFKIGRPGSVRAL
                     EEALSTDRTIFLATQHDASVDEPAPELIYGVGTIARILQAQRQENGQTKVVVEGRERA
                     TTIRIENDGGAFTAIVRRAPVVNESGARVEALVQRINQLVEQHLRLAPEAQTDALQTA
                     LRNQDSSHLADALASQIKISVEDKQGLLEIFSTTGRLQR"
BASE COUNT         3464 a         6874 c         6880 g         3618 t
ORIGIN      
        1 cagcgcgtcc accacgctga agacggtcgt gttcgcgccg atgcccaaag cgagcgccag
       61 caccgccacg agcgtgaagc ccggcttctt ccacatcatc cgaaagccgt agcgaatgtc
      121 ttgcagcagt gttcccatga caatctccaa agaggtcaga ggttagaggt cagaggttag
      181 tgaagacagt ttttaactga cctctaacct ctgacctctg acctctattc gtatctcagc
      241 gccaccatcg gatcgacgcg cgtggcgcgg cgggccggga tgtagcaggc gaggagggcg
      301 accaacccga gcagcacggc gacgcccgcg aaggtctgcg ggtcggtggc cgacacgccg
      361 aagagcaggc ccgacatcac gcgcgtcagc aggaaagcgc ccgtcagccc gatgaccacg
      421 ccgagggcgg cgagcttggc cccctgtccg acgaccatcg aggtgatgtt gccgggctgc
      481 gcgcccaagg ccatgcggat gccgatctcg tgcgtgcgct gcgcgaccgt gtacgacatc
      541 acgccgtaga ggccgatgac ggcgagcacc atggcgagcg cggcgaagga gccgagcagg
      601 agcatggaga agcgccgctg cgcgagcgag tcggagacga gttgctccat cgtcgtcacg
      661 cggaagacgg gcaggtcgcg gtcaatcgag gcgaccgcgc cgcgcaccgc gcccgccaga
      721 ctcgtcgcct cgccggccga ggggcgcacg gcgaggaaca tgctgccctg cgcgagctgc
      781 gggtgcggga tgtagtactg cacgcgcgac tcgccctcca gccccttgtg cttgacgtgg
      841 ccgacgatgc cgacgatttc gcgccagcgc ggattggcag cgccgccttc gtagctgatg
      901 cgcttgccca tcgggtcttc gtcgggccag tacttgcggg ccatcgtctc gtcgatgatg
      961 gcgacgggct gcgcgtccga gaggtcgcgc tccgtgaaga agcgcccgcg cacgaggggg
     1021 acgcccaacg tcttgaagta gtcggcagtg gcggcccagc ggtcgccgtg cggcaacgac
     1081 gcgccctgcg gaacctcacg cccctcgatg cggaagctgc cgctctggtt gttgccgctc
     1141 atcgggagga tggcggtcgc gccggccgaa cccacgccgg gaatggcgcg cacctgctcc
     1201 atcaactgct gatagaaggc gtcgcgctgc tgcggctctg ggtacttgct gcccggcaga
     1261 gacacctgca tcacgagcag gttctgcggg tcgaaaccgg ggctgacgtc ttgcaggcgg
     1321 tagaagcttc tgatgagcag gcccgcgccg actaagagca ccacggcgag cgacacctcc
     1381 gcgatgacga gcgcgccgcg cacgccgcgt cgcatcacgc ccacgccgct gcgtccgccc
     1441 tccttgagcg tgtcgtggag ttcgatcttg gaagtctgga aggccggggc gaggccgaag
     1501 acgatgccgg tgacgagcga gacgccaagc gtgaagagga cgaccgtcag gtcgaggccg
     1561 atctcgtgcg cgcgcggaat cttgtcctga ttgatggcta ccagaaactc cacgccccac
     1621 agccccaaca gcaaaccgac tccgccgccg atgacggaca acagcgcgct ctccgtgagc
     1681 aactgtctca cgatgcgccc gcggctcgcg cccatcgccg tgcggatggc gatctccttc
     1741 tgccgcgccg ccgagcgcgc cagcatcagg ttggcgacgt tggcgcacgc gatgagcaga
     1801 accaaagcga ccgcgccgag aaggatgagc agcgccgggc ggatgtcgcc gacgaccgtc
     1861 tcgttgaagg aggtgagcag gaggccccag tcggcggccg tcatgccggg cgagtactgc
     1921 gtccgcaggt tgtcggcgat gttatccatc tcgacctgcg cctgcccgat ggagacgccg
     1981 ggcttgagac gcgcgagcac cgccagactc tcgttcgtca ggttgctcat ctggagctgc
     2041 tggggcgtcc aacccaccgg cgcccacacc tcgacgagcg gccccaactc ccggccgaac
     2101 tggaagccgg gcggcatcac gccgacgacc ttgtggctct cgccgttcaa ggtgaggtct
     2161 ttgcccacga ttccggcgtc ggagccgaag cgacgctgcc agagcgcgtc gcttaagacg
     2221 acgaccttgt tgttgccggg ctgattctcc tcagccgtga aggtgcgccc gcgcgcggcc
     2281 tcggccccaa gtagtgggaa gaggtcgtgt gtgacggcca tgccttgcag ccgctccggc
     2341 tcgccgcccg tccccgtcag gttcgcgttc caacccgtca ccgcgccgac gccctcgaac
     2401 gactgcgcgt tgtcgcggta gtgcgagtag cccggcacag agacggacgc cttcaggttg
     2461 atcttctggt agttgtgatt aatcaacacc agctcgccgg ggtctttgta ggggagcggc
     2521 cggagcagga tggcgttgac gaccgagaag atggccgagt tggccccgat gcccaaagcg
     2581 agcgcgagca cggcgacggc cgtgaagccg gggcttttgg tgagcatccg aatcccgtag
     2641 cgcaggtctt gcaagatagt gtccatcaca actccaatca ttcttcactc gtgcctgagt
     2701 gcttccatcg ggtctacctt cgtcgcgcgc agcgccggga cgaagcaggc cacgaaggcg
     2761 accgccgcga gcagcacggc gacgcccgcg aacgtcaagg ggtcggacgc gctgacgccg
     2821 tagagcagcc ccgacatggc gcgcgtcaaa agcaaagcgc ccaacagacc ggcgcccatc
     2881 cccgcggccg tgagccacat cccctgcccc acgatcatgc ggagcacgtc gcggccttgc
     2941 gcgccaagcg ccatgcgaat cccgatctcg tgcgtgcgtt gcgagacggt gtaagacatc
     3001 acgccgtaga ggccgatgac ggcgagcgcg agcgcgacgg ccgcgaagac gccgacgacg
     3061 agcgtgttga agcgacgcgg cgcgaccgac tcggcgacga tgcgctccat cggcttcacg
     3121 tcgtagaccg gctgattttt atccacggcc cacacctcgc cgcggacggc gcgggcgagc
     3181 gcttcggggt cgccgcccga ggggccgcgc acgacgagcg tcatcacctg cgagggcgtc
     3241 tggaggtggt gcaggtagat ctgcaagttc gcctccttct ccagcccgta gtggcgcacg
     3301 tcgccagcca cgccgacgat ctcaacccac gggttcttca cgtcgccgaa ctttatgcgg
     3361 cggcccaagg ggtcttcatt cggccagaag cgttcggaca tggagcggcc gatgacggcg
     3421 acgagcggcg cgccctcgcg gtcgcgcgcg tcgaaggcgc ggcccgcgac gagcggcacg
     3481 ccgagcgcgc ggaagtagtc ggcgctgacc atgtagcgtt cggcgtcggg cgcgtcgccg
     3541 gcgagctgtg cgggctggcc ctcgacgtag acgggcatcc ggtcgtagtt gccgctcacc
     3601 gggaggttgc tcgtcgcgcc ggcagactcg acgccgggca gcgccgccgc gcgctcgacc
     3661 accgcgtcga agaaggccgc gtgctgcgcg tcctcggggt agcgcgcgcc gggcaggaag
     3721 acgttcatcg tcaccgcgcc gcgcgggtcg aagccgggat cgacggcgag caggcgaacg
     3781 aaacttctga caagcaggcc cgcgccgact aagagcacga gcgacagcgc cgtctcggca
     3841 acgacgagca gggcgcgcgc ccggcgccgc gcggccggct gcccctggct ccggccgccc
     3901 tccttcagcg cctcgttcaa atcgagacgc gtggactgga tggccgggac gagtccgaag
     3961 gcgacgcccg tcagaagcga cgccgcgagc gtgaagacga gcacgcgcgc gtcgaggccg
     4021 acgcccgcga tgcgcgggat ggagtccgcg ccgagcgcga gcagaaggtc gttggcccac
     4081 cacgcgaaga gcagccccaa cagtccgccc atgagagaga gcatcacgct ctccgtgagc
     4141 atctgccgca cgactctcag ccgcgacgca ccgagcgccg tgcgaatggc aatctccctg
     4201 cgccgcgccg acgcgcgcgc taggctaagg ttagcgacgt tggcgcacgc aatcaggagc
     4261 acgaagccga cggcgacgag gaacgtgagc agcgcgcggc gcgagccgcc gaccagttgc
     4321 tcgtgcagcg agacgaggcg cacgcccgcg ttcttgttcg tttcggggta ctgcgcttcg
     4381 agccgcgcgg cgatggtcgc catctcggcc tgcgcctgct ccacagtcac gcccggcttg
     4441 agacgcgcga ggcacgcatc aaccgagcga ctgctgcgcg tccactgcgc gaggtcgggc
     4501 gcgaagggcg cccacagctc cggcggctca gtggccccca gccccgtgaa ctccggcgac
     4561 tcgaactcgc gcgccgcgac gccgacgacc gtgaaggctt cgccgttgag ttcgatctgc
     4621 tggccgacga cgttctgcgc tccgccgaag cggcgctgcc agaggccgtg accgaggacg
     4681 acgacgcggt gttggccgcg ctgctcctct tgcgggagga agccgcggcc cacggcgggt
     4741 tcagacttga ggacggagaa gaaggacgcg gagacgcggg ccgcgttgat gcgctcaggc
     4801 tcgcccgcgc ccgtcagtga gggcgaccag ccgtcgtagg cggcgacggc ctcgaacgac
     4861 gtgctctgct cgcgccagtc gaagaagtcg gccgggctgt gcgtgccgat ggcctcggcc
     4921 gcgcgcgcgc tcgtcccgcc gactctcacc agccgctccg gctcggggta cggaagcggc
     4981 cgcaggagta cggcgttgac gaccgagaag atggccgagt tggccccgat gcctaaggcg
     5041 agggcgacga cggcgacgag cgtgaacccc ggactcttcc agagcatccg cacgccgtac
     5101 ctgatgtcct gccagagggt tcccatcatc actcccgaga ggtcagaggt cagaggtcag
     5161 taaaaaccgc ttcttcttta actgacctct aacctctgac ctctgacctc tgttttcacg
     5221 ctgcttccac ctgctcttcg ctgacgacgc ggccgtcgaa gagatagatg gcgcggtcgg
     5281 cgaacttggc atagcgcggg tcgtgcgtga ccatgcagat ggtcgagccg acctgatgca
     5341 gctcgcgcaa aagcgccatc acggcctcgc cgttggtcga gtcgaggttt ccggtcggct
     5401 cgtcggcgag gaggatgagc gggtcgccgg cgatggcgcg ggcgacggcc acccgctgct
     5461 gctggccgcc tgagagctgc gaggggaggt gcttggcgcg gtgcgccatg ttgaccttct
     5521 ccagcgcctc gcccacacgc ttcttgcgct cggctcccga catgccgcga taggtcaggg
     5581 gcagctccac gttctcgtag accgtgaggt cgccgatgag gttgaagctc tggaagatga
     5641 agccgacctc gcggttgcgg atgacggcgc gctcggagag cgacagctcc gacacgtcct
     5701 tcgagttgag ccagtagtgg ccgtcggtcg gggagtcgag gaggccgagg atggagagca
     5761 gtgtcgattt gccgcagccc gacgggccgg tgatcgagac gaactcgccg ctctggatgt
     5821 cgaggtaggt gccggaaagc gcgtgtgttt ccacctcgtc ggtgtagaag accttcttca
     5881 cgtcctccag gtggatcagg ggtttgccgt tgtcaggtgc agtgctcatt agtttctcct
     5941 ggtgataagt gatgagtgat aagtgatgag tgataagaag gaagacggat tgtttttctt
     6001 atcacttatc actcatcact tatcactaac tgggttcact tcaattcaat gtggttgaag
     6061 gcgtcccact gcgacgtgtc cgagaggatc aactggtcgc cctccttcag cccttcgacg
     6121 atctcgatgg tgttgacgga gctgcggccg aggcgcacct gcacgcgcgt ggcggacgag
     6181 ccgccctcgt cgagcttgaa gatgccgacg gtggcgttgg cctgcccgaa ggtgggccgc
     6241 gcgacgtaga ggatgttctc cagtcgctcc agctcgatgg tgccgtccac gctcaggtcg
     6301 gggcgcgcgc ccgcaggcaa agcgccttca agctgcacgt ccacggccac ggtgccgccc
     6361 tgcgcggccg ggtcgatgcg cgacacgcgg cccgggatga cgccgtttct ggtgtcgatg
     6421 gaggccggct ggccgatggc gatgccgctc gtctgcgtct cggcgatgcg tagctcggcc
     6481 ttgaggttct gcggctcgac gacgcgggcc agaaccgtgc ccggcgtcac ctcctgcccg
     6541 acttcgacga ggcgttgctg caacacgccg ttcgtcccgg cgagcacgtg caggctcgcc
     6601 gcctgttgcc ggcgcaggcc gagttccgcc ttgagctggt tgacgcgcgc cacctgcgaa
     6661 cccatctgcg cctcgtcaga cttcttgcgg ccttcgaggc ggcgctgctc gatggcgtag
     6721 cggttggcga gttcttcggc gcgcacgcgc gacaggcgcg tgttgagttc ggggatgaga
     6781 ccttctttgg cgagcgtctg gtcggtgtcg gcgatgatct tggcctgctg gtactcggcg
     6841 cggacggtgg cggcggccgc ctgctgggcc atgcgctcgt tatcgaggcg cgcgcgaatg
     6901 ttgttgaggt cggcctcggc cgagaggagt tggtactgaa catcgacgac ctgctgctgg
     6961 agttcggggt tggcgagttc gatgaggatg gtgccggcgc ccacgtcctc gccgggctgg
     7021 acgaggatgc gctcgacgcg cccctgcgtg gaggccgcga cgacgcgcac ttcttcgggg
     7081 acgagcgtgc cgatgccgcg cacctgccgg agcatctcgc cgcgcttgac ggtatcgatg
     7141 agcacggtcg agcgctgcac gccgggcgcg gccggcttca tgcgcgagac ggcgaaggtg
     7201 atgccggcga tggcggcgac ggcgaccacg gcgagtccga tgttcttgac gcgccgcttg
     7261 cgcgccgccg acttcttgcg cggcacgtcc atcgtgttgc tcggcgtggt gaagccggtc
     7321 ttgcggggct gatcctgtcc cttgcggggc acgtcctgtc cctcgccctt ctcgcttcgg
     7381 atgggagata tcttcgtgtt catgtcttct ttgtcgtcgg ccacgccggg gcgtgtttcg
     7441 tcgttgcttg gcaagatgtt tactaaaacg aaaagctggc gaccggcgtc gaagcctcta
     7501 acgacggcga cgcccgtctt tgttccgccc gcgtcaggcg gcgctcgtct gtccttcgtc
     7561 ggccatccag ccgtccacca gattgatgac gcggttgccg taggaagcgt tcgtctcgga
     7621 gtgcgtcacc tgaatgatgg tggcgccggc gtcgttgagg cccttgaaga gttccatgat
     7681 ctcgcggccc tgacccgagt ggaggttgcc ggtcggctcg tcggcgagga tcagtttggg
     7741 gctggcgatg acggcgcggg ccacgccgac gagttgctgc tgcccgcccg agagctggtt
     7801 ggggaagagg tcttttttgc cgacgatgtg gaagcggtcg agcgtgtcgg cgaccatcga
     7861 ctggcggtcg cccttcttga tgtccttgta ggtgagcggc agctccaggt tctcgtagac
     7921 cgtcaggtgg tcgagcaggt ggtagctctg gaagacgaag ccgatatgcc ggttgtggag
     7981 gtcgttgcgc tccttgcgat tcaaggcgtg gacgggcttg tccatgaagt agtactcgcc
     8041 cttccacgcg ctatcgaaca tgccgaggat gccgagcagc gtggacttgc ccgcgcccga
     8101 aggccccatg atggaaacga actcgccctc gcgcacgtgc atgttgatgc ggcgcagaac
     8161 gaagttcttt gtgttgccct gctcgaaagc tttttcgacg ttgcgcagct caatcatccg
     8221 ttgtttctcc ggccggctct tcggcggtat tctccacgag cgagagaact ccttatggaa
     8281 gtggcgtgcc agaacgggtg aatcgaattg tgcgggtgtt tcgggcggcc gcgcgcgagc
     8341 ggcgggttgc gggtttcgcg tttttgggaa tcgacagttc caaaaatgaa ccggccggcg
     8401 ttcaaaaaaa tgttcgtgcg tccaaaaaga agggcggccg cgcgtggcga ccgcccttcc
     8461 agaagtgttt cgtttgacgc gtgtggccgg ctcggtctta accgacgctg ccgccgccgc
     8521 tgccggactt gccgccgctg ccctgcgact cgccgccttg tgtgccgccg ccttttgagc
     8581 cggccccgcc ggtcgcatcc gaagtctcga cctcagtgcg ctcgaccccg actgcctctt
     8641 cgagcggctc gacggcgccg acgaccgcgc ccttcaccgc cccgctcgcc gcgcccgcga
     8701 tcatctcgcc cgccactttc gcggcccgct tgatgaggcc cggcttcttg ccgcccttcc
     8761 tgccgccgcc gcgcttcttc gcggacttct tcgccccgga cttcttactc gcgccactct
     8821 tcttgctccc gcctttttta gcgcccgact tcttggcacc cgacttcttc gcggccgact
     8881 ttttcgcacc ggacttcttc gccgcggact tcttagcgcc ggatttcttc gcgccgccct
     8941 tcttgcccgc gccgcccttg cgcgccccgc ccgacttgcc gccggatgac tttttggccg
     9001 ccgacttctt cttcgcggac ttcttcccgc tccccgattt cctgctcgct ttctttgtcg
     9061 ccattactct ctcctccttc actcctccgt gtagtttttc tgtaatgctt cgtggaacct
     9121 atcaaactac acccgccatg tcacgaacat ttgtccgcgg cgcgcgacgt atcttcgtcc
     9181 gccccgtaca ttccgaaatc cgctcgcccc ccgccgcccg tacatcgctt tcgtgagacc
     9241 tgaagttgac atctccacct gagttgaaat aaacaccgcc cacggcgcgg ggccgccccg
     9301 ccgctccgac tctggattcg acccgaactg ccttgacccg caagaagact cttcagcagc
     9361 gttgggagtt cgacctccgc tccagccccg aggcgctctg gccctacgtg gccgacacca
     9421 accgcttcaa cagcgacacg ggactgcccg ccgtagcagt ggccgacgat gaagcgggcg
     9481 acgaggccac caccggcgcg ccgaggaacg cgcgccgccg gctgcgcgtg aaggtctccg
     9541 tgttggagat gctctacgaa gaggagccgt tcgagtgggt gcgcccgcac ggattcggcg
     9601 tcgtgcgccg ctacgcgggc ggccccatcg cggagatgcg cgtccgcgtc gagctacggc
     9661 cgcgcgaggg cggcggcacg catctcgtct acgacaccga gttcaccccg cgcgcatcgg
     9721 tcgtccgtct cgccctcccg ctccagctca aactgatgcg ccgaaagttc gagcgcgcct
     9781 tccgacgcta cgaccgtctc gccgccgaac gcgcgaccgc cccacagacc gccgcgacgc
     9841 cgaacgccgc cgccccactc tccgaacgca cgcagctcac ccccacgggc cgcgagagac
     9901 ttcggcagat cggccgcgcg ctcgtcgccg aagggaagaa cgcggaactc gtccggcgac
     9961 tctccgagct tgtcgagcgc ggcgacgacc tcgcgctcgc gcgcgtgcgg ccctacgcgc
    10021 tcgccgacct gtggacggcc gaccgccgcg aagtgctcga actctgcctc gccgcgacgc
    10081 gcgccggcct gctcgacttc cgctgggaac tcctctgccc cctctgccgc ggctcggccg
    10141 actctcccgc ctcactcgga gacatcaccg ccggccagca ctgcgcgacc tgcggcatcg
    10201 acttcgaggt caacttcgac cgctccgtcg aactcacctt ccgccccaac cccgccgtgc
    10261 ggaaggtcga aggcgcagcc ttctgcgtcg ccggccccgc gacgacgccc cacgtcgtcg
    10321 tccagcaact gctccgtccg ggcgagtcgc gcacggtgcg cccgcggctc gaagagggcc
    10381 gctatcgcct gcgcgcgctc gggctgagcg gcgcgcaaca agtccgcgtc gcgggcgcgg
    10441 gcgcgggcgg cgtagtttca ttaggcccga cggaggcggc ccgttggccg tcggacgaac
    10501 gtctgatcgg agtcgagccg gccctgcgca tcgagaacaa ggacgcggtc gagcgtctct
    10561 tcatcctcga acgcacttcg tggagcgacc aggcggcgac ggccgccgag gtgacggcgc
    10621 tgcaagtgtt ccgcgacctc ttcgccgaag aggcgctgcg gcccggcgag cagatttccg
    10681 tcgggagcct gacgctgctc ttcacagacc tgcgcggctc cacacagctc tatcgacaga
    10741 tcggcgacgc cgtcgccttc ggcgccgtga tgacccactt cgacgtgctg cgcgaatcaa
    10801 tcgcggcgga aggcggcgcc atcatcaaga cgctcggcga cgccgtgatg ggcgctttca
    10861 cacgccccgc gccggccttg cgcgccgcca tgcgcgcaca ggcgatgctg gccgcgccgc
    10921 ccgcgggcgt gcgccccctc gcgctcaagg tcggcattca caccggcccc tgcatcgccg
    10981 tcaccctcaa cggccgcctc gactacttcg gctccaacgt caacatcgcc gcccgcctcg
    11041 aaccgctctc gtccggctcc gactgcatca tctcgcgccc cgtccgcgac gacccgaaga
    11101 tcgccgccct gctctcgggc caggcgagcg gcctcgtcgc cgaaccccta cgcacgaccc
    11161 tcaaaggctt cgacgccgag cacttcgacc tctggcgcgt gaccagaaag gaaatctctc
    11221 cgccggaaac ggagacgccg acgaaacgcg gaatttgatg tgagggtagg tggggcaagc
    11281 cccgtccggg gcggaagaat tcgttcgtta ctcaatcgca gttggtgtcc gactcctcgg
    11341 ggtagacctt gaggtctctc tccgaggtga tgcgccagcg gccgtcgatg cgggcgaggc
    11401 gaagctgctg ctgcaccttc cccttcgagc accgctcgcc ctcgaacttc cacttcttat
    11461 cgagcgtgac ctgcgcgccc gcgcccgtct cgtcgagcgc gatgctgatg ttgtcgatgt
    11521 ccacgttgat cttctggtag cggtcgaagg cgcgcgcgcg gtcggtgcgg acgttcgtgc
    11581 gcgccacgcc gcgcgagttg taatagcctt cgagcgtgtc ggcgtagaag ctcatgtgct
    11641 cgtcgaggtc gcgatcctcg gtcgatgaca cccagccgtc aagcgcgtcc acgacctcgc
    11701 ggcgcatctc cttctgatcg ggcggaggcg tcggggccgg gccggcgccc gcgccgacga
    11761 gcaggcggat gttggcgcgg tcagcctccg tcagacgttc gtccgtgtag tccccgcttt
    11821 gcttgtacca cggtcgcgtg cggaagtgtg cgcgtaggtc tacgggggtg aaggcccggc
    11881 cgtggcgcgc gtagacggtg ttgagcagga ggcgcagcgc cgcgttgtcg aggcccgcca
    11941 gctcggtctc gacgagacgc atgttgctga gcaccttcgc ctccgcgcgc tggaaggccg
    12001 ccagttgggc gttggtgttg gagttcgtat tggtgttggt gttggtgttg gtgttcgtgt
    12061 tcgccgacgt gttgtgttgc gccgtgcgat tcgggcgcgt gttccggttc gcgttgagga
    12121 acatgtaaaa gccgaagccg gcgaccgcga tggccgcccc gcacaggagc atcaggccga
    12181 cccagaaggg cgcgcgcgac ttacgccggg gcggaggctg gggcgcggcg tgcgggacgt
    12241 gcggcgcggg gcctgccgtg tggacggcgg gcggctggcg accggtcgag gcggcggcgg
    12301 gaagcgtcgt cggctgtggc ggcgctccgg catgtggatg cgccgcgttc gcttcggggc
    12361 cgagcgcggt cgtggcggcg ccggcggcgg cggcggtcga aagctcacgc gagaactcgg
    12421 aggcgtcggt ctgtcgggcg ttcgcatcct tggcgagcgc gcggtggacg gtctgctcga
    12481 tggaggcggg gatgcgcgcg tcggccggca cgcgcggcgg cggctcgctc agatgtttgg
    12541 agatgacgcc cgtcggcgtg gccgccgtga agggcggcgc gccggcgatc atctcgtaga
    12601 agagcgcgcc gagggaatac acgtcggccc gcgagtcgag cggctcgccg aggcactgct
    12661 cgggcgacat gtagtgcggc gtgccgacga cggcgccggc ggccgtcagc gtgtgatcgg
    12721 ccgacgcgtc gcgcagcttg gcgatgccga agtccacgac cttcacggtc tcgcgctcgc
    12781 ggtcgtcgtc gggcgcgagc acgatgatgt tgtcgggctt gatgtcgcgg tggacgatgt
    12841 gccggcggtg ggccgaggcc acgcccgcgc acacgtcgcg catcagggag acggcgcgcg
    12901 tgaggtcgag tcggccctcg cgcttgagca gttcgcgcag cgagacgccc gccaccagct
    12961 ccatgacgat gtaggcgagc ggctgcccgc ccgtctgggt ctcgccgtag tcgtggatgg
    13021 tgacgacgtt gtggtgatgg agctgcgcgg cggcgcgcgc ctcacggcgg aagcgttcga
    13081 cggccgacgc gtcggcgacg aacttcgggt ggaggatttt gaccgccacc tcgtcgccga
    13141 tgtggacgcg ccgcgcgcgg tagaccgccc ccatgccgcc ctcgccgagc cggcgctgca
    13201 actcgtactt gccgtcgagc acctgccccg tgagcacgtc ggccgagacg acggtcagct
    13261 catcttccag acgcacaacg ggcgccggcg cgctctcgcg cgtcgtctcc gacggggggt
    13321 cgcccgcgcc agcattgatg cgctcgccgc aacccgtgca gaactgcgcg cccccccgca
    13381 gctcggtctg acagttcgga cagttcagat tttctcaaga ggtcagaggt cagaggttag
    13441 aggtcagtga aagacaagat gctctttact gacctctaac ctctgacctc tgacctcttc
    13501 gctttttcgt gtcccgtcac gcgggactaa agtttggcct tatcaaccct cgatggcgtc
    13561 taagacgctc gtcgctttct tctcgtaggc gagaatggcc gagacgttct tcttctcgac
    13621 cgtggtcagc gccgcctcct tgaaacgggg gtcgggtttg taccagccga ggctctcgaa
    13681 atatttctgg agccagcggt ctttgaagac cttgccgcgg cgcgcgagga tttcgtggcg
    13741 gagcttgcgc gcgtcttcga ggaacaggcc gtcgaggagc ttggtcgaga cgggcttcgt
    13801 gctcaactcc ccgcggatgc ggttctcgta gccgacgatc agctcgatgt tctttcgctc
    13861 gatgtcggag agttccggct ccttgaactc ctcgctctgc tccggctcgt accacggctg
    13921 gctgtagaag tgctgcgaga gccactcggt gcggaagcgc cggccgcgcc gcgcgtagac
    13981 ctcgttgcgg agcaggcgca actcgtggag gctgaggccg tcgagcatcg cttcggagac
    14041 gggcgtcttc tggaagtgct ccatgtcgcc gggcgagagc gcgacgttgc gctgcttgcg
    14101 ctgggcggcg tcgatggtcg tcatgttgcg ccgctcgatg tcggtcaaaa gcttcgggtc
    14161 ataccccgcg ttcgcctgat accagtagcg atcctcgaag tactgctgga gccacggctg
    14221 gtcgtcgaag cgcttgccgt ggacggcctc gatctcggcc cggaggatgc gccactcggc
    14281 gccgttgtgc tcgccgagtt gtttggcgtt gaaggcgcgc gcctgccacc agcgcatgtc
    14341 gcccggctcg atcacttcgt ggagacgcgc ctccatctcg cggatgaggt cgaggttgcg
    14401 gcgctccgtg tcgttgagcg aggagttcga gaagttcggg tctggcttgt accagggccg
    14461 ctccttgagg tagatgccga tgtcccagtc tttgaagacg cgcccgtggc gtccgaagac
    14521 tagccctcgc aggagtttca agtcttcgag cggcatgtcc ttgagttgtt cgcgcgtgac
    14581 gagctgcttc gcgaagtcga agctctccca tttcttgacg gcctcctcgt cggtcgcgcc
    14641 cgcggctgcc ggcgcgagga cgagcagcgc gaagaggagc gccgcgtgtc gtagtagatg
    14701 tctcttcaac atgggggaaa cgttcctttc aggcgtcctg agttttgtca cccgtcggtt
    14761 ttgttacccg tcaggcttta agggtcttat tatccgcgcg gcccgtagcg aatggcaccg
    14821 cggggagaca caacgagccg gccgggctgc cctcagttct ttccgcgagg cgccgcttga
    14881 acgcgcgggc cgacgaaacg tgcgatgccg ccggcaatcg cctcggccat cttctgctgg
    14941 cccgcctccg acttgatgaa ctccgcgtcg cccgcgtcgc ttaagacgac catctcgacc
    15001 gtgacgaccg gcacgtccga aaagatcgag ccggtgagcg cgccgccctg ctcgcggccg
    15061 accttcgtct ggtagtccgt tcgcacgccg ttgtcgtcga gttggccttc caacacttcg
    15121 ctcatccccg cgtggatggc gcgggccgcg cgagcgctcg cctcgaccac gcgcgccgag
    15181 gggccgaccg cgccgtcctt cgtgcgcccc ggccggtcgg ggtagtagac ggcaaacccg
    15241 cgctcgatgc tcgcgtcgca gtgcaggcgc accatgagcg cggcccgcgc gcggttggcg
    15301 accgtggcgc ggtcgcggtt gcgcacgagt tcgtcctcgg ccgacttcgt caacacgact
    15361 tcgtagcctt ccgtctcaag ttgcgtcttg agcttgaggg cgacctccca ggccacgcgc
    15421 gtctcgttcg tgccgttctg ctcgccgcgg ccgctggcga cctccgacgg gtggccggga
    15481 tcgatggcga taatcgtccg cgcggcgcgc gccttcaccg ccgccacagt cgccggactg
    15541 gccacggcct tcggctcggc cttgcgctct tgtgctacgg cgagcgcgcc gaggggccac
    15601 gccagcagca gcagcagggc gagtgcgttg gcgtcgagtc tcatgtgcgt ccgtgtgcgc
    15661 cgcgtcgtcc ttaatttcat cgagtgaagc agcaatcaag tctgcggggc attatttgac
    15721 ggcgtcgccg cgatgtcaac aacgtctcga ctacacaccc cgcgcgccct ccgacgcacc
    15781 tgccgccgcg ggtgtttgac ctgccccggc atgcccgcga atgtgtccgt tgtgctgcta
    15841 gacattcaaa cttcattcct ttaatctccc ttgttcctac ctcacttgtg gagctgcgta
    15901 atgagaacgg acattattga aaggcgtctg gagattgaaa actggatagc gagtcgccgg
    15961 cctaaagctt tcatgtgtcg cgagttgcaa tgtaagccat cgacactcaa cagctatttg
    16021 cagaagatgg gcttaaatta cgccggcaac atgggtggtc agggctataa gcaatgcccg
    16081 acccgcaagg gtgcgcgcat atatttacgt aaaggcactc tcattagtag ctataagctg
    16141 aagcgaaaat tacttgagga cgggctgaag aaacgccgat gtgaacaatg tgagctgaca
    16201 gaatggttaa gccagcccat cccgattgag ttacaccaca taaacggtga tagattcgat
    16261 aacagattcg gaaatttaaa actactttgc cccaactgtc atgcgctcac tgataatcac
    16321 gcaggcaaag ggtcaaatcg gattaaggtt tattccaaca actgtaacta ggacatgcga
    16381 aagtggcgga attggcagac gcgccaggtt taggccctgg tcccgaaagg ggtgggggtt
    16441 cgagtccccc ctttcgcatt aaagttttga gagatgtcgg tcgcagagat taatagataa
    16501 cgaggaagga cgcccgcggc ttcagggctg gcaccttggt ctgaggcggc gggcttttgc
    16561 gtttatgaag acagaaatcg ttgacgtgtc ggcgacgcgc aaggagttca agatcgagat
    16621 cgacgccgcc gaggtgcgcg ccgagtatga ccgcgtgagc ggcgagtacg cgcgcaaggc
    16681 gaccgtgccg ggcttccgca aggggcacgc gccggcgtcg gtcgtgcgga cgcgctacaa
    16741 gaaggagatc agcggcgagg tgctgcaaaa gctcgtgccc gaggccgtca accgcgcgct
    16801 cgaagagagc ggcctgcgcg tgctcggcca gcccgatgtg catctcgcgc ccgagagcat
    16861 cgaacgcttg ggcgaagccc ccgtctcgct ccacgcgcac gtcgaggtgc tgcccgacgt
    16921 ggagttgggc gagtacaagg gactctcggc cgcgcgccgc acgcgccccg tcaccgacga
    16981 gatggtcgag caaacgctcg cgggcctgcg cgagtcgtcg gcctcgctcc agccggtcga
    17041 ggaacgcggc gcggagtcgg gcgacaccgt caccgtcaat ttccacggcc gcttcgtcga
    17101 acccgcgggg gcggaggaca tcacggccga cgatgtggac gtggtgctcg gcggcgaggg
    17161 cgtgcaggaa gactttaacg ccaacctcat gggcgcccgc gccgacgaag agaagacgtt
    17221 caccgtgaag taccccgagg acttctcctc gaagggactg gccggcaaga cggttgatta
    17281 cacggcgaag gtcgtcgcgg tgagccgcaa ggagttgccc gaactcgacg acgagtgggc
    17341 gaagtcgctg ggcgaggaga tcgactcggt cgagtctctg cgccggcgcg tgcgcgagaa
    17401 ccttgagggg cgcgcgaagt tcgaggccga ggggcgcgtg cgcgacgagg tggtcggccg
    17461 cctcgtggac gcgcacaagt tcgagctgcc cgagtcgctc gtcgagtacc agaccaacca
    17521 gatgctccag acggccgtgc gcgacatgat ccgccacggc ctcgacccgc gcgccgagca
    17581 gttcgactgg gaggcgctgc gcggcggcgc gcgggagcgc gccgaggagg aactgcgcgc
    17641 ctcgatgctg ctcgaaacca tcgcggagcg tgagaatatc gacgtgacgg acgaggaggt
    17701 cgaggaagaa atcaagatga tcgcggaggc gtcgcgacag acggtcgagc aggtgcgggc
    17761 cgccttgaca aagcaggggg gcgccactag catcgccgat agcttgcgca accgcaaagc
    17821 ggtcgatctt ttagtcgaaa aagcgaacgt cagcgaggaa gaatggcgcg aggaaaagca
    17881 ggagtcagag gtcacaagtc aggagtcgga ataagcgtcg cgacggattt attcttctgg
    17941 attgcggact cctgaattct atggaagggt tgatatggca ttagtcccaa tggtcgtcga
    18001 gcaaacgtcg cgaggcgagc gcgccttcga catcttctcg cggctcctca aagacaacat
    18061 catcttcatc ggcacgccca tcgacgacca gatcgccaac ctgatcgtcg cccagctcct
    18121 cttcttagag gccgaagacc ccgagaagga catcaacctc tacatcaact cgcccggcgg
    18181 ctcgatcacg gccggcatgg ccgtctacga cacgatgcag tttatccgcc ccgacgtgac
    18241 gacgatctgc gtggggcagt gcgcgtcgat ggcggcgctg cttttggcgg ccggcgcgca
    18301 cggcaagcgc ttcgccctgc ccaactcccg catcctcatc caccagccct cgggcggcgc
    18361 gcagggtcag gccagcgaca tccgcatcat ggccgaggaa atcctccgca tgcgcgagtt
    18421 gacctcgcgc atcatcgcca agcacacggg ccagacctac gaggtcatcg agcgcgacgt
    18481 tgagcgcgac cgcatcatgt cggcggcgca ggccaaggag tatggcctca tcgacgaagt
    18541 catcgcgcac cgcgaagcct gaagtcccac acgaacgcgc tcgccccccg cgcgaaaagt
    18601 tctttacaag tttgcctttc gggcgtacat ctgttgtaag atggcgcccg cgtccgcgtg
    18661 ggcgcgccag cgaacgcgcc ggggccgact ctctcccgaa ttgctcgaca cgcgcccggc
    18721 ccgccacgct cgcacgcgaa gagacaaccc aagctcgacc cgaaaccctg agacgtcccg
    18781 gcaatacccc ctcgaaggaa cttatggtac gacgaaccga cgacacgttg cgctgctcct
    18841 tctgcggcaa gtcgcagaac gaggtcaaga agctcatcgc aggccccacc gtctacatct
    18901 gcaacgagtg cattgacatc tgcaacgaga tcatcacgga cgaccagcag gccgaggccg
    18961 tcgcctctcg cccgccgctc cccaaaccgg ccgagatcaa aaccttcctc gacgagtacg
    19021 tcatcggtca ggacgagacg aagaagcgcc tcgccgtcgc cgtctatcag cactacaagc
    19081 gcatcgacat cgcgcgccgc cgctccgatg tcgagattca gaagtcgaac atcctcctca
    19141 tcggcccgac cggcaccggc aagacgctgc tcgcgcagac gctcgcacgc atcctcgccg
    19201 tccccttcac catcgtggac gcgacgactc tgaccgaggc cggctatgtc ggcgaggacg
    19261 tggagaacat catcctcaag ctcttgcagg ccgctaacgg cgacgtggag cgcgcgcaac
    19321 agggcatcat ctacatcgac gaggtcgata agatttgccg caaggacgag aacccgtcga
    19381 tcacgcgcga cgtgtcgggc gagggcgtgc agcaggcgct gctcaagatt ttggaaggca
    19441 ccgtggccaa cgtcccgccg cagggcggac gcaagcaccc gcatcaggag ttcttcccca
    19501 tcgatacgac gaacatcctt ttcatctgcg gcggcgcgtt cgtcgggctg gagaaggtca
    19561 tcgagcggcg tctcaggaac aagtcgatgg gcttccaggc cgaggtcaag tcgagcggcg
    19621 cgcgccacgc cgaggcgctc ggcttcgttc agcccgaaga cctcatccgg ttcggcctca
    19681 tccccgagtt cgtcggccgc ctgcccgtct gcggcgtgct ccacgaactc gacgaggcgg
    19741 cgctcatccg tatcctgacg gagccgaaga acgcgctcat caagcagtat cagaagaagt
    19801 tcgagtacga caacgtcaag ctgcgcttca ccgaagacgc gctgaaggcc atcgcccgcc
    19861 aggccgtcga gcgcaaggtc ggcgcgcgcg gcctgatgat gatcatggag gagatgctcc
    19921 tcgaagccat gtacacgctc ccctcgcaga agcgtatcaa ggagttcgtc atcaccagcg
    19981 agatggtcga gcgccgtcgc gccgttccgg gcgaagtgct gaccggggtt gacgaagccg
    20041 cgtaagacaa ggcaaaagta aaaaggcaaa aggcaaaagt aaggagggcg cttctccact
    20101 tttgcctttt gcctttttac ttttgccttg aaagtccggt ggcacggatt cacaaaatag
    20161 tctaaacttg gacgtgtgcc ggcgaccgtt gacgaacgtc tcgcctcgct gcccggcacg
    20221 gacacgggaa ttattagatt atggaagaat actctgaccg cacccctccc gacatcgcgc
    20281 ggctgccgat ggtgcccatc cgtgatgtcg tcatcttccc cttcacgaag gtggcgttca
    20341 agatcggtcg gcccggctcg gtgcgcgccc tcgaagaggc gctctccaca gaccgcacca
    20401 tcttcctcgc cacgcagcat gatgcctccg tcgatgagcc ggcccccgaa ctgatttacg
    20461 gcgtcggcac catcgcccgc atccttcagg cccagcgtca ggagaacggg cagacgaagg
    20521 tcgtcgtcga ggggcgagag cgcgccacga ccatccgcat cgagaacgac ggcggcgcgt
    20581 tcaccgcgat agtccgccga gcgcccgtgg tcaacgagtc cggcgcgcgg gtcgaggcgc
    20641 tcgtccagcg catcaaccag ctcgtcgagc aacatctgcg gctcgccccc gaggcgcaaa
    20701 cggacgcgct ccagaccgcg ctccgcaatc aggattcctc gcacctcgcc gacgccttgg
    20761 cttcccagat caaaatctcc gtcgaggaca agcagggtct tctggaaatc ttctccacga
    20821 cgggccgcct tcagcg
//
LOCUS       DASVES010000476         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1062883, whole genome shotgun sequence.
ACCESSION   DASVES010000476 DASVES010000000
VERSION     DASVES010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062883"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..909
                     /locus_tag="VF240_19955"
     CDS             55..909
                     /locus_tag="VF240_19955"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7177543.1"
                     /translation="MKSEMRIGVASLALLLALSPTAATAAAQKESKEPPKQSPQGEVK
                     AAHPTPTPGERLEPDDTTVARPVASKEMLANRIGQRSEEEAAVLPFYNNFLTQYRLGP
                     EDVISISVFGHDRYSKAGITIPPDGNLSYYLIPEGILVAGKTTQQVAQELAKHLDEYI
                     IDPKVTVTLEKAQSHQFSVLGDVAQPGIRVMSRRLTVYEALLAAGGVLGTGNKKKVVL
                     VRHGPDRMLTQQLIDVAAIEKGRAPDNTFLAPGDQIFVPGNRMKTVNKIIELLPVISF
                     ARIFTGGF"
     gene            1090..2082
                     /locus_tag="VF240_19960"
     CDS             1090..2082
                     /locus_tag="VF240_19960"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX7177544.1"
                     /translation="MPFCATAQSGWKRQPTGTFAWLHAVHFIDGERGWAVGGKGALLQ
                     TTNGGRDWKLMPAPCEDTLRDIHFADAHTGWLVAERDIYKLRTEDETRAYLLKTTDGG
                     ANWSRVEVVGKETNARLVRVRFAGSEHGWVFGEEGALFATSDGGRTWARQLVPTRHLL
                     LGGAFRDARVGWLVGAGGTFLHTSDGGATWGAALLVAASSISTTAAQFAPSGSRAPRV
                     NAVSFIDERRGWAVGSNGSVFSTTNGGRSWSARTTGTDADLLDVKFFDEREGFAAGAG
                     GAVLHTTDGGVTWVLQPTDTTHQLERLHFLDRRQGWAVGFGGTILTYDAASQPK"
     gene            2391..3191
                     /locus_tag="VF240_19965"
     CDS             2391..3191
                     /locus_tag="VF240_19965"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7177545.1"
                     /translation="MKRSLLRLSLCSSVLLLVSAASVAAQDFNRSYQLAPGGSISIRN
                     VSGNIEVSGHDGDQVVVNGTKVGPDVDMVEVEDLSGPGAVDVRAKYPRNCRRCDVSVN
                     FTIQVPRNTRLSFGPISTASGNIKLTNVTGDVKVNTASGDVLVSQVTGDINASTASGE
                     MSVRNVSGTVNANSASGNVEVDIDRLEGGGNMRFSSASGDVRVRVPSSVDADVYMSTA
                     SGAIKTNFPIEVREDRHGSGSRAEGRLGSGSRSLRISTASGNVSLTSN"
BASE COUNT          609 a         1138 c         1063 g          513 t
ORIGIN      
        1 acataaggct cggactcttc ggagttgtta acggaagttt tggaggttaa gacgatgaaa
       61 tcggaaatga gaatcggcgt cgcatcactc gcgctcctgc tcgcgctttc gccgacggcc
      121 gccacggccg cggcgcaaaa ggagtcgaag gagccgccga agcaatcgcc gcagggcgag
      181 gtgaaggcgg cacatcccac gccgacgccg ggcgagcgtc tggagccgga cgacaccacg
      241 gtcgcccgcc ccgtcgcctc caaagagatg ctcgccaacc gcatcgggca gcggagcgag
      301 gaggaggccg ccgtgcttcc cttctacaac aacttcctca cgcagtaccg gctcggcccc
      361 gaggacgtga tctcgatttc ggtcttcggc cacgaccgct actcgaaggc cggcatcacc
      421 atcccgcccg acggcaatct ctcctactac ctcatccccg agggcatcct cgtcgccggc
      481 aagaccacgc agcaggtagc gcaggaactc gccaagcacc tcgacgagta catcatcgac
      541 ccgaaggtga cggtcacgct ggagaaggcg cagtcgcacc agttcagcgt gctcggcgac
      601 gtggcgcagc ccggcatccg ggtgatgtcg cgccgcctca ccgtctacga ggcgctgctg
      661 gcggcgggcg gcgtgctcgg cacggggaac aagaagaagg tggtgctcgt gcgccacggg
      721 cctgaccgga tgctcacgca gcagctcatc gacgtggccg ccatcgagaa gggccgcgcg
      781 cccgacaaca ctttcctcgc gcccggcgac cagattttcg tccccggcaa ccgcatgaag
      841 acggtcaaca agataatcga actgctcccc gtcatcagct tcgcgcgcat cttcaccggc
      901 ggattttagg ggaaggcaaa agtaaaaagg caaaaggcaa aagtgggaag gacaggccga
      961 aggcttcagg caatgagaga cggaggatgg cagagacgag ggcggatagt tggcgtcgcg
     1021 tgcgaacgcc gtcgtcgtca ccccggcctt cgtcgcgctc ttttactttt gccttttgcc
     1081 ttttgccttt tgcccttctg cgcgacagcg cagagcggat ggaagcggca gccgacgggc
     1141 accttcgcgt ggctgcacgc cgtccacttc atcgacggcg agcgcgggtg ggccgtcggc
     1201 ggcaagggcg cgctgcttca gacgacgaac ggcgggcgcg actggaagct gatgcccgcc
     1261 ccttgcgaag acacgctgcg cgacatccat ttcgcggacg cgcacacggg ctggctcgtg
     1321 gccgagcgcg acatctacaa actcaggact gaagacgaga cgcgcgccta tcttttgaag
     1381 acgaccgacg gaggcgccaa ctggtcgcgc gtcgaagtcg tgggcaagga gacgaacgcg
     1441 cggctcgtgc gcgtgcgttt cgccggctcg gagcacggct gggtcttcgg cgaggagggc
     1501 gcgctcttcg ccacgtcgga cggcggccgg acgtgggcgc gccagctcgt gcccacgcga
     1561 cacctgctcc tcggcggcgc cttccgcgac gcgcgcgtgg gctggctcgt cggggcgggc
     1621 ggaaccttcc tccacacgtc ggacggcggc gcgacgtggg gcgcggccct tctcgtcgcg
     1681 gcctcttcca tcagcacgac ggccgcgcag ttcgccccgt cgggctcgcg cgcgccgcgc
     1741 gtcaacgccg tctcgttcat cgacgagcgg cggggctggg cggtcgggtc aaacggctcg
     1801 gtcttctcca ccaccaacgg cggacgctcc tggagcgcgc gaacgacggg gacggacgcc
     1861 gacctcctcg acgtgaaatt cttcgacgag cgcgaaggct tcgccgccgg tgcgggcggc
     1921 gccgtgttgc acacgaccga cggcggcgta acctgggtgc tccagccgac cgacaccaca
     1981 caccagctcg aacgactcca cttcctcgac cgccggcaag gctgggccgt cggcttcggc
     2041 gggacgattc tcacctacga cgcggcgtca cagccgaagt aggcaagggg aacacggatt
     2101 aaacggatgg gacggatgag cacggattcg atctgtgtaa atccgtccca tccgtttaat
     2161 ccgtgttccc tcccgcattt cccgccgccg gatttttttt gccacaaacc ccgcgccccc
     2221 ggtgtttcca cgtcagagcg gatggaaaac gacgcgcacg ccggtggacg aaccaacttc
     2281 ggccccggac gaaagtcgtc gcttccttac aaggccgaag catcttcgcg acccggacgc
     2341 tctctcgtat tccacacacc gaagtttttc acaggaggat ttccgcagcc atgaaaagaa
     2401 gtttgctccg tctgagtctc tgctcgtccg ttcttttact cgtctccgcg gccagcgtcg
     2461 ccgcgcagga cttcaaccgt tcctaccagc tcgcgcccgg cggctccatc agcatccgca
     2521 acgtctcggg caacatcgag gtgagcgggc acgacggcga tcaggtcgtc gtcaacggca
     2581 cgaaggtcgg ccccgacgtt gacatggtcg aggtcgaaga cctctccggc ccgggcgccg
     2641 tggacgtgcg tgccaagtac ccgcgcaact gccgccgctg cgatgtcagc gtcaacttca
     2701 ccattcaggt gccgcgcaac acgcgcctct ccttcggccc gatctcgacg gcctccggca
     2761 acatcaagct gacgaatgtg acgggcgacg tgaaggtcaa caccgcgagc ggcgacgtgc
     2821 tcgtcagcca ggtcaccggc gacatcaacg cttcgaccgc cagcggcgag atgagcgtcc
     2881 gcaacgtgtc gggcaccgtc aacgccaaca gcgccagcgg caacgtcgag gtggacatcg
     2941 accgcctcga aggcggcggc aacatgaggt tctcctcggc cagcggcgat gtccgagtcc
     3001 gcgtgccctc ctccgtcgat gccgacgtat acatgtccac cgccagcggc gccatcaaga
     3061 cgaacttccc catcgaagtc agggaagacc gccacggctc gggcagccgc gccgagggcc
     3121 gtctgggcag cggctcgcgc agcctgcgca tctcgacggc ctccggcaac gtgagtctga
     3181 cgagcaacta aacggttccc gcgtgggtcg taccaagacg actcacgcgc gcgaagcgca
     3241 gggggcgaca ggggaagcgc gccggggctg gccgccgacc tcttcgcccc ctacgcgcgt
     3301 ccgccagcga caatgagccg cgc
//
LOCUS       DASVES010000477         8596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_918129, whole genome shotgun sequence.
ACCESSION   DASVES010000477 DASVES010000000
VERSION     DASVES010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918129"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..2039
                     /gene="uvrB"
                     /locus_tag="VF240_19970"
     CDS             51..2039
                     /gene="uvrB"
                     /locus_tag="VF240_19970"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX7177546.1"
                     /translation="MPFKLRSEFKPQGDQPAAIDALGRGLDGGVKDPVLLGITGSGKT
                     FSVAPVIDRVQRPALVIAHNKTLAAQLYQEFKSFFPENSVEYFVSYYDYYQPEAYVPA
                     SDTYIEKESTVNEEIDRLRLAATRALFERRDVIVVASVSCIYGLGDPDAYYKMLLFVE
                     PGQRMTRDELLQKLVELQYERSDIEFDRGSFRVRGDVVEVYPSYQDQAYRIELWGDEI
                     DSVSTIDPLLGEVLHKHTSRLPIYPKTHYVMPKATVKRAIKTIREELDWWEPKLIQEG
                     KLVEAQRLHQRTMYDLEMIKEMGFCRGIENYSRHLTGKPPGSPPPTLLDYLPEGTIAV
                     IDESHQTMPQVRGMFNGDQSRKRTLVEYGFRLPSAMDNRPLNFQEFEERIGQTIYVSA
                     TPGPYELLRSGGEVVEQIIRPTGLTDPVVDVRPVKGQIDDLLEECRLRAERNERVLVT
                     TLTKKMSEDLTEYFAEVGVKVRYLHSDIETLERIKILRDLRRGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSESSLIQTMGRAARHSSGKAILYADKMTDSMRRAIGET
                     DRRRGIQLEYNAEHGITPQTIVKPIEATLVTAYEADYFKVPLDLDQIDDYSPAKLNET
                     IARLEGEMRLAAKALEFERAAELRDRVKYLHERKIQLG"
     gene            2246..3814
                     /locus_tag="VF240_19975"
     CDS             2246..3814
                     /locus_tag="VF240_19975"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7177547.1"
                     /translation="MLVKSEADEIQSFLSDASNLAGGHASRVVVPETAEEAAEALASA
                     ARERVSVTVAGAGTGIVGGRVPFGGVVLSTHKFNCIGEVIREGAGGRVSVGAGVPLID
                     LQRASDAAGLFYPPDPTEWSCSVGGTVATNASGARTFKYGPTRSYVRRLKVALTTGDV
                     LDLRRGEVRADASGAISIPLRGGRTIDARLPSYTMPRTRKHAAGYYAAPGADAVDLFV
                     GSEGTLGVVTEVEVGLLPRPEGVLCGIVFFREEDDLLACVNEAREQSFATRARTEASA
                     ASGGVPAGDVRGRSAVEPCGLDARALEYFDAESLNFLRERFALVPLSTAGALFFEQEI
                     TEAAEDRLMQSWLRLLERHGALLDDSWFGANEYDRAEMREFRHQLPVMVNEWLARHNQ
                     RKVSTDTAVPDEAFPALLRSYKQALRAKRLQYVVFGHVGDSHVHVNIMPRDDIERLSA
                     LDLYAQFIRQAVAAGGTVSAEHGVGKLKREYLRVLYGEPALREMAALKRAFDPSCVLG
                     RGNIFDEELLGSDK"
     gene            3890..5059
                     /gene="aroC"
                     /locus_tag="VF240_19980"
     CDS             3890..5059
                     /gene="aroC"
                     /locus_tag="VF240_19980"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX7177548.1"
                     /translation="MFRFTTAGESHGRALVAIVEGLPAGMPVDVEALNRELERRQWGY
                     GRGGRMKIERDRAEILSGVRHGLTLGSPVALQIENKDWGNWSEVMAAEPREAPEEKRR
                     RVRRPRPGHADLAGGLKFGARDLRDILERASARETAARVACGALARQLLSAFGVELKS
                     HVVQLGGVPDAPLEVAWEAIASIPDDAPLRCADAEAQERMVALIDEKKRAGDTLGGVF
                     EVVTRGLVAGLGSHVSWDAKLDGRLAQALMSIPAVKAVEIGAGVGAASRPGSEVHDEI
                     DYDTEARRFVRPTNRAGGLEGGMTNGEELRARGYLKPISTLRQPLRSVDIDTKEEERA
                     AFERSDVTAVPAAGVIGEAMTALVLASAMREKFGGDHLDEMRRNFDGYREQLRQY"
     gene            complement(5337..5822)
                     /locus_tag="VF240_19985"
     CDS             complement(5337..5822)
                     /locus_tag="VF240_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177549.1"
                     /translation="MTRTLLIAFLLLSTAACAQTSRRGEGVAAIETPPRPTPTAKSAA
                     TPPPVDESAKKEKGAGVFDRYKFTFEKTGERVTADFPSPKLPYNDNVVVGAAREVILT
                     AYGEKMENFPRRVGWNFEGAQHAIMLAGDSYDYVFVHAADADKQIPRLVFWRVAKGSV
                     D"
     gene            6106..6516
                     /locus_tag="VF240_19990"
     CDS             6106..6516
                     /locus_tag="VF240_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177550.1"
                     /translation="MSDLRHPKSKRADVMAVALALLGLAGLSVSASDLVGGPALWPSF
                     SGEHVYTARLSPAEADAYTPHVVTYAAPRQPASDEVALRWINEAARGVCQSEAGARRQ
                     TRFDASRRTRRQRTLSRRALTLLTATTSGRPANS"
     gene            6584..>8596
                     /locus_tag="VF240_19995"
     CDS             6584..>8596
                     /locus_tag="VF240_19995"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177551.1"
                     /translation="MSELKRRYARRASLCLLALLAGGSATTFAQQPTPSPTPAPEPTP
                     QESRPETDASVENKMFDRLEWRSIGPANMGGRTSDVEGVAGNPNLVYVATASGGLFKT
                     TNGGVRWTPVFERQGSISIGDIALEPGNPDVIWLGTGEANPRNSVSFGDGVYKSTDGG
                     KTWQHVGLRDSRHVSRILINPRNPEVVYVGALGHAFGPNEERGVFMTTDGGRTWAKTL
                     YVDAEHGVADMEIDPTNPNILYAAVWKFRRTPWTHTSGSEKGGVFKSTDGGRTWKKLE
                     GGLPKTIGRIGVKAAPSNPSVVYAITEAKEGTLWRSDDRGETWRNVSKETRIVSRGFY
                     YTDLRVDPTNENRVYAVASTLFVSIDGGRTFRSITGRTHIDYHALWIDPQNPRRMWQG
                     QDGGVAVSYDAGETWEYVNNFPAGQFYQITADNRQPFYYVMGGLQDNGTWTGPSRTRE
                     PAGILNDDWRMVSFGDGFYAINHADNPDLYLTESQGGNIVRTDMRTREQQLVVPSMGS
                     DDGPAMFAKYRFNWNSPIIQSPHDKDTVYLGGNVVFKSTDFGKTWATISPDLTTNDKE
                     KQKDAGGPVAFENTGAEYHSTVISFAESPLKAGVLWAGTDDGQLQLSTDGGKNWTNLT
                     KNVSGLAANSPVSHVEPSRVALETAYVSFDRHMLDDLRPYVYKTDDG"
BASE COUNT         1551 a         2939 c         2706 g         1400 t
ORIGIN      
        1 gctaacggcc gttttacgat tcacgattta cgatttacta ttcacgagtt atgcctttca
       61 agcttcgctc cgaattcaag ccgcaggggg atcagccggc ggccatcgac gcgctggggc
      121 gcggactcga tggcggcgtc aaggatccgg tgttgctcgg catcaccggc tcgggcaaga
      181 ccttctcggt ggcccccgtc atcgaccgcg tgcagcgccc cgccctcgtc atcgcccaca
      241 acaagacgct cgccgcgcaa ctctatcagg agttcaaaag tttcttcccc gaaaactcgg
      301 tcgagtactt cgtctcctac tacgactact accagccgga ggcttacgtc ccggcatccg
      361 acacctacat cgagaaggaa tcgacggtca acgaggagat cgaccgcctg cgcctcgcgg
      421 ccacccgcgc cctcttcgag cgcagggacg tgatcgtcgt cgcctccgtc tcctgcatct
      481 acggcctcgg cgacccggac gcctattaca agatgctcct cttcgtcgag ccgggccagc
      541 gcatgacgcg cgacgagctg ttgcagaagc tcgtcgagtt gcagtacgag cgcagcgaca
      601 tcgagttcga ccgcggctct ttccgcgtgc gcggcgacgt ggtcgaggtc tatccctcct
      661 atcaggatca ggcgtatcgc atcgagctgt ggggcgacga gatcgactcg gtctcgacca
      721 tcgacccgct gctcggcgaa gtcctccaca agcacaccag ccgcctgccc atctacccca
      781 agacgcacta cgtgatgccg aaggcgacgg tgaaacgcgc catcaagacc atccgcgagg
      841 aactcgactg gtgggagccg aagctcattc aggagggcaa gctcgtcgag gcgcagcgcc
      901 ttcatcaacg caccatgtac gaccttgaga tgatcaagga gatgggcttc tgccgcggca
      961 tcgaaaacta ttcgcgccac ctcacgggca agccgcccgg ctcgccgccg ccgacgctcc
     1021 tcgactatct ccccgaagga accatcgccg tcatcgacga gtcgcaccag accatgccgc
     1081 aggtgcgcgg catgttcaac ggcgaccagt cgcgcaagcg cacgctcgtc gagtacggct
     1141 tccgcctgcc ttcggcgatg gacaaccgcc cgctcaactt tcaggagttc gaggagcgca
     1201 tcgggcagac catctacgtc tcggccacgc ccggccccta cgaactcctg cgttcgggcg
     1261 gcgaggtcgt cgagcaaatt atccgcccga cggggctgac tgaccccgtt gtcgatgtgc
     1321 gccccgtcaa gggacagatc gacgacctgc tcgaagagtg ccgcctccgc gccgaacgca
     1381 acgagcgcgt gctcgtcacc acgctcacca agaagatgtc cgaagacctc accgagtatt
     1441 tcgccgaggt cggcgtgaaa gtgcgctacc tccactcgga catcgagacc ttggagcgca
     1501 tcaaaattct gcgcgacctg cggcgcggcg agttcgacgt gctcgtcggc atcaacctcc
     1561 tgcgcgaggg cctcgacctg ccggaagtgt ccctcgtcgc catcctcgac gccgacaaag
     1621 agggcttcct ccgcagcgag tcctccctca tccagacgat gggccgcgcc gcccgccact
     1681 cgagcggcaa ggccatcctc tacgccgaca agatgaccga ctcgatgcgc cgggccatcg
     1741 gcgagaccga ccgccgccgc gggattcaat tggagtacaa cgccgagcac ggcatcacgc
     1801 cgcagacgat tgtgaagccg attgaggcca cgctcgtcac cgcctacgag gccgactact
     1861 tcaaggtgcc gctcgacctc gaccagatcg acgactactc gcccgccaaa ctcaacgaaa
     1921 ccatcgcccg tctcgaaggc gagatgcgcc tcgccgccaa ggccctcgaa ttcgagcgcg
     1981 ccgccgagct gcgcgaccgc gtcaagtacc tgcacgagcg caagattcag ttggggtaaa
     2041 gggaagaatc attaacatgg atggacagaa cgggcaggat gaagaaagag ctgatttgag
     2101 atttcagatt caacatttga gatttcaaat ctgcttatcc cgtccatcca atccatccat
     2161 gttaattcct ctgtaggacg ggccgcggac ttgccctgcc cctacagtcg tgacgcacgt
     2221 tgaaggagtt ggcaacggac tattgatgct ggtcaaatcc gaagcagacg agattcaatc
     2281 ctttctgtcg gacgcgagca acctcgccgg cggccacgcc tcgcgcgtcg tcgttcccga
     2341 gacggccgaa gaggccgccg aggctttggc ctccgctgcg cgcgaaaggg tgagcgtcac
     2401 cgtcgccggc gcgggcacag gcatcgtcgg cggtcgcgtc cccttcggcg gcgtcgtgct
     2461 ctccactcat aaattcaatt gcatcggcga agtcatccgc gagggcgcgg gcggacgcgt
     2521 gagcgtgggg gcgggcgtcc ctttaatcga cctccagcgc gcgtcggatg ccgcgggact
     2581 gttctacccg cccgacccga ccgagtggag ctgctcggtg ggcggcacgg tggcgacgaa
     2641 cgcttcgggc gcacgcactt tcaagtatgg gccgacacgc tcatacgtcc gacgcctgaa
     2701 ggtcgcgctc acgacgggcg acgtgctcga tctgcgccgc ggcgaggtgc gcgccgacgc
     2761 gtccggcgcg atcagcatcc ccttgcgcgg cgggcggacg atagacgcgc ggctgccttc
     2821 atacacgatg ccgcggacgc gcaagcacgc cgccggctac tacgccgcgc cgggtgcgga
     2881 cgccgtcgat ctgttcgtcg ggagcgaggg cacgctcggc gtcgtcaccg aggtcgaggt
     2941 agggctgctg ccgcgccccg agggcgtcct gtgcggcatc gtcttcttcc gcgaagagga
     3001 tgacttgctc gcctgcgtca acgaggcgcg cgagcagtcc ttcgcgacac gcgcgcgcac
     3061 cgaggcgagc gcggccagcg gcggcgtgcc ggccggcgac gtgcgcggcc gcagcgccgt
     3121 cgaaccgtgt gggcttgacg cacgcgcgct cgaatacttc gacgccgagt cgctcaactt
     3181 cctgcgcgag cgcttcgcgc tggtgccgct gagcaccgcc ggcgccctct tcttcgagca
     3241 ggaaatcacg gaggccgccg aagatcgcct gatgcagagt tggctccgcc ttctcgaacg
     3301 ccacggcgcg ctcctcgacg actcctggtt cggcgccaac gagtacgacc gcgccgagat
     3361 gcgcgagttc cgccaccagc tccccgtgat ggtcaacgaa tggctcgcgc gccacaacca
     3421 gcgcaaggtc tcgaccgaca cggccgtgcc cgacgaagcg ttccccgcac tgcttcgctc
     3481 ctacaaacag gcgttgcgcg ccaagcgcct ccagtacgtc gtcttcggac atgtcggcga
     3541 cagccatgtc cacgtcaaca tcatgccgcg cgacgacatc gagcgcctgt ccgcgctcga
     3601 cctctacgcg cagttcatcc ggcaggccgt cgccgcaggc ggcaccgtct cggccgagca
     3661 cggcgtcggc aagctcaagc gcgagtacct gcgcgtgctc tacggcgagc cggccttgcg
     3721 cgagatggcc gcactcaaac gcgccttcga cccgtcttgc gtcctcggcc gcggcaacat
     3781 cttcgatgaa gagcttcttg ggagtgataa gtgatgagtg ataagtgata aaaagagaaa
     3841 agcggatttt cttatcactt atcactcatc acttatcact gacttaaata tgtttcgctt
     3901 caccaccgct ggagagtcac acgggcgcgc gctcgtcgcg atagtcgagg gtctgccggc
     3961 ggggatgccg gttgatgtcg aggcgttgaa ccgcgagctt gagcggaggc aatggggcta
     4021 cgggcgcggg gggcggatga agatcgagcg cgaccgcgcg gagattctga gcggggtgcg
     4081 acacgggctg acgctggggt cgcccgtcgc gctccagatt gagaacaagg actgggggaa
     4141 ctggtcggag gtgatggcgg ccgagccgcg tgaggcgccg gaagagaagc gtcggcgcgt
     4201 gcgccggccg cggcccggcc acgccgacct cgcgggcggg ctgaagttcg gcgcgcgcga
     4261 tttgcgcgac atactggagc gcgcctctgc ccgcgagacg gccgcccgcg tcgcctgcgg
     4321 cgcgctcgcc cgccaactgc tctcggcctt cggggtcgaa ctcaaaagcc acgtcgtgca
     4381 gctcggcggc gtccccgacg cgccgctgga agtcgcgtgg gaggccatcg cctccatccc
     4441 cgacgacgcg ccgttgcgct gcgccgacgc ggaggcgcag gagcgcatgg tcgcgctcat
     4501 cgatgagaag aagcgcgcgg gcgacacgct cggcggcgtc ttcgaggtgg tgacgcgcgg
     4561 cctcgtcgcg gggcttggct cgcacgtctc ctgggacgcg aagctcgacg ggcggctggc
     4621 gcaggcgttg atgtcgatcc cggcagtcaa ggccgtcgag atcggcgcgg gcgtcggcgc
     4681 ggcctcgcgt cccggctcgg aggttcacga cgagatcgac tacgacaccg aagcgcgcag
     4741 gttcgtgcgc ccgaccaacc gcgccggcgg tctcgaaggc ggaatgacga acggcgagga
     4801 gttgcgcgcg cgcggctatc tcaagcccat ctcgacgctg cggcaaccgt tgcgctccgt
     4861 ggacatcgat acgaaggaag aagagcgggc ggccttcgag cgttcggacg tgacggccgt
     4921 tccggcggcg ggcgtcatcg gtgaggcgat gacggcgctc gtgctggcct cggcgatgcg
     4981 cgagaagttt ggcggcgacc acctcgacga aatgcgccgc aacttcgacg gctaccgcga
     5041 gcagttgcgc cagtattgaa gccacttagt cgccgcgcgc cgccagccgc cggaggcggc
     5101 gaaagaatgt agccacgggc ggccgtcccg tgggatccgt cgtagaaaca aatcaacaag
     5161 ccccggaggg gcgaaagact cgcgcgtgcg ttatatgtag cgcgattctt tcgcccctcc
     5221 ggggcttgtt gactttaatc cggcgtgtcc ccacggctag cgccgtgggc tacaatcttt
     5281 cgccgctccg cggctgattg aaacgttctt cctagtctgc cttttcaatc cgcaattcaa
     5341 tctacggaac ctttcgccac ccgccagaag accagccgag gaatctgctt gtcggcgtcg
     5401 gcggcgtgga cgaagacgta gtcgtaactg tcgccggcga gcatgatggc gtgctgcgcg
     5461 ccctcgaaat tccagccgac gcggcgcggg aagttctcca tcttctctcc gtaggctgtg
     5521 aggatgactt cgcgcgcggc gccgacgacg acgttgtcgt tataaggcag cttcggagaa
     5581 gggaagtcgg ccgtcacgcg ctcgcccgtc ttctcgaacg tgaacttgta acggtcgaag
     5641 acgcccgcgc ccttctcttt cttcgcgctc tcatcgacgg gcgggggcgt ggcggccgac
     5701 ttcgcggtcg gtgtcgggcg gggcggcgtt tcgatggcgg cgacgccttc gccacgtcgc
     5761 gaagtttggg cacaggcggc cgtggagagc agcagaaatg cgatcagtag cgtcctcgtc
     5821 atcgttcttc gtcgtcgccg cgtgaaggtc gtcgcgtttg ccggtcaatt ctaacgtcgc
     5881 ggcggggcgg gcgcgaattg cggggccgaa atccttcggc ggacggcccg ttcgtcccga
     5941 agtcgaaccc tttcgtcaca ttattatgcc ggcgtgcggg ccgttcgttt tacaattact
     6001 ttacgctttg atgcgttccc tgcaacgccc gccgcagcgg gccacacggc cggggacgga
     6061 ttcgccgaaa ggctcacacg ggcggtcgaa aagaaggaca aggacgtgtc tgacttaaga
     6121 catccaaaat ccaaacgcgc ggacgtgatg gctgtcgcgc tggccctgct cgggcttgcg
     6181 ggcctcagcg tctcggccag cgaccttgtc ggcggccccg ccctgtggcc gagcttctcc
     6241 ggcgaacacg tctacaccgc gcgcctgtcg ccggccgaag cggacgcata caccccgcac
     6301 gtcgtgacct acgccgcgcc gcggcagccc gcctccgacg aagtcgccct gcgctggatc
     6361 aacgaggccg cgcgcggcgt ctgccagagc gaagccggcg cgcgccgtca aacgcgcttc
     6421 gatgcgagcc gccgcacacg tcggcagcgc acgctctcgc ggcgcgcgct gacgctcctc
     6481 accgccacca cgtctggacg accggcgaac tcatagttta tcctgccgac tccgtctcgt
     6541 tctctatacc cagccgaaaa ttctagctct caggagtcga agcatgtccg aactcaagcg
     6601 ccgatacgcc cgccgggcgt ctctctgtct tctcgccctg ctcgcgggag gctctgcaac
     6661 caccttcgcg cagcagccca cgccttcgcc cacgcccgcg cccgagccga ccccgcagga
     6721 gagccggccc gagacagacg cctcggtcga gaacaagatg ttcgaccggc tggagtggag
     6781 aagcatcggg ccggcgaaca tgggcgggcg cacgtcggac gttgagggcg tcgccggaaa
     6841 tccgaacctc gtctacgtcg ctacggcctc gggcggtctc ttcaagacga cgaacggcgg
     6901 cgtgcgctgg acgcccgtct tcgagcggca aggctcaatc tccatcggcg acatcgcgct
     6961 tgagccgggc aaccccgatg tcatctggct cggcacgggc gaggccaacc cgcgcaactc
     7021 cgtctcgttt ggcgacggcg tctacaagtc cacggacggc gggaagacgt ggcagcacgt
     7081 gggcctgcgc gacagccgac acgtctcgcg tattctcatc aacccgcgta acccggaggt
     7141 ggtctacgtc ggcgcgctcg gccacgcctt cggccccaac gaggagcgcg gcgtgtttat
     7201 gacgaccgac ggcgggcgca cgtgggcgaa gacgctctac gtggacgccg agcacggcgt
     7261 cgccgacatg gagatcgacc cgaccaaccc gaacatcctc tacgccgccg tctggaagtt
     7321 tcgccgcacg ccgtggacgc acacgtcggg gagcgaaaag ggcggcgtct tcaaatcaac
     7381 ggacggcggc cggacgtgga agaagctcga aggcggattg ccgaagacca tcgggcgcat
     7441 cggcgtgaag gccgcgccgt cgaacccctc ggtcgtctac gccatcacgg aggccaagga
     7501 gggcacgctc tggcgctccg acgaccgagg cgagacgtgg cgcaacgtct cgaaggagac
     7561 ccgcatcgtc tcgcgcggct tctactacac agacctgcga gttgacccga cgaacgagaa
     7621 ccgcgtctac gccgtcgcct ccaccctctt cgtctccatc gacggcgggc gcaccttccg
     7681 ctccatcacc ggccgcacgc acatcgacta tcacgcgctc tggatcgacc cccagaaccc
     7741 gcgccgcatg tggcagggtc aggacggcgg cgtcgccgtc tcatacgacg cgggcgaaac
     7801 gtgggagtac gtcaacaact tcccggccgg tcagttctat caaatcacgg ccgacaaccg
     7861 gcagccgttc tactacgtga tgggcggcct tcaggacaac ggcacttgga cggggccgtc
     7921 gcgcacgcgc gagccggccg gcatcctcaa cgacgactgg cgcatggtct ccttcggcga
     7981 cggcttctac gccatcaacc acgccgacaa ccctgacctc tacctcaccg agtcgcaggg
     8041 cggcaacatc gtccgcaccg acatgcgaac ccgcgagcag cagctcgtcg tcccctcgat
     8101 gggcagcgac gacggccccg ccatgttcgc caagtatcgc ttcaactgga actcacccat
     8161 catccagtcg ccgcacgaca aggacaccgt ctacctcggc ggcaacgtcg ttttcaagtc
     8221 aacagacttc ggcaagacgt gggcgaccat cagccccgac ctcaccacga acgacaagga
     8281 gaaacaaaaa gacgcgggcg gccccgtcgc tttcgagaac accggtgccg agtatcactc
     8341 gaccgtcatc agtttcgccg agtcgcccct aaaggcgggc gtcctctggg ccgggaccga
     8401 cgacggccag ctacagctct cgaccgacgg cggcaagaac tggacgaacc tcacgaagaa
     8461 cgtgtcgggt cttgcggcca actcgcccgt ctcgcacgtc gagccttcgc gtgtcgcact
     8521 ggagacggcc tacgtctcct tcgaccgcca catgctcgac gacctgcgcc cctacgtcta
     8581 caagaccgac gacggc
//
LOCUS       DASVES010000478         7539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_482555, whole genome shotgun sequence.
ACCESSION   DASVES010000478 DASVES010000000
VERSION     DASVES010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482555"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /locus_tag="VF240_20000"
     CDS             complement(<1..2156)
                     /locus_tag="VF240_20000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177552.1"
                     /translation="MNNLWQDVRYGARMIAKRPLLTLITVAALAVGIGANTAIFSVVN
                     AVLLRPLPYPESERIVRVFSTDAKRNARKYPTSYLNFVDWREQSSVFTQTAAHASTGA
                     AVNFGDTPESVEGVYASADLFPLLGVSPALGRTFTREEERPGSNVVVLSHGIWERRFG
                     GDPAVVGRQILFDGESTTVVGVMPEGFKFPVDVEEVQYWTPFNPETPTNKERGNNYLS
                     VVARLKPGVSVEQGQAEMEAVTGRLEEQYNTSNAGRGANVVSMYEDLVGDVRPALYVL
                     LGAVGFVLLIACANVANLLLARAASRQKEIAIRTALGASRWRVVRQMLTESLLLALIG
                     GALGLLLALWGVDALSALIPADIPRVKDIGLDTRVLLFTLGVSVLTGVVFGLAPALQA
                     SKPDLNESLKDGSRGSTEGFGRNRLRALLIVSEVALSIMLLVGAGLLVRSFLELRSVK
                     PGFEAQNVLTAGISLPSAKYGEEQQQAAFYSQALERIAALPGVKAVGGVEPMPFSGNG
                     WQTSLKFPDRPPAAPGERPTSHARIVSPDYFRALGIPLQRGRVIAETDDARAPKVMVI
                     NETFASKYFPGEDPVGKRVTPSVAPGYDTQIVGVVGDVKHRRLNEDAAPEFYISYQQA
                     PGPILALVVRSEGGDPAGLANAVRQAVLQVDPNQPLYSVKTMQELLSDSVARSRFQML
                     LLTIFAAVALALAGVGLFGVMSYTVTQRTHEIGIRMA"
     gene            complement(2248..4668)
                     /locus_tag="VF240_20005"
     CDS             complement(2248..4668)
                     /locus_tag="VF240_20005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177553.1"
                     /translation="MGTMLQDLKYGFRMLLKTPGVTFIAILALALGIGANTAIFSVVN
                     AVLLRPLQYRESERLAIMWEKTPKMDTSVAYPNFKDWREQQTVFDAVAAGRLHSFNLT
                     GTGEPERLQGRMVAHEFFGVLGVEPFKGRDFTADEDRTGANRTAILGHGFWQRRFGGD
                     QSIVGKELTLNSQSYTVIGITPPDYRYGTDTDVFVPLGLFESEYAERGAHPGIYVLGR
                     MKPGVTLEQARAGMDTLMAQLEQAYPKTNQDRRIHIESLYENTVSDVRPALYVLLGAV
                     GFVLLIACANVANLLLARSASRQKEIAIRTALGAGRWRIVRQLLTESVILSLVGGAAG
                     LLLALWGTDLLIAAVPDNIPRLSEAGVDTRVLVFTLGVSVLTGVVFGLFPALQASRPD
                     LNESLKEGERGSTGARHRLRNALVVTEVAVALVLLVGAGLMVRSFWRLQSVDTGFDPK
                     GILTMRLSVAAKDGEGQKAMNFIDDVTARVRALPGIEAAAFTTGLPFAGASEQSFWVD
                     GRPRADEREFMMAVMYTTATGYFEAMNIGLKKGRYLDERDRSGSALVCVIDESLAAKA
                     FPDEDPIGQRLTIGPPEAPPYEIVGVVEHVKHYGLEGEVPVDPQFYFSLAQIPEPFLP
                     RMVGRGTIVVRTKGDPLALAGQVRQQVLAADPNQPVFNTRTMEEIVANSTGQRRFAMT
                     LLTIFAGVALLLAAVGLYGVMSYTVTQRTHEIGIRMALGARGGDILGMVVRQGMVLVI
                     LGVVIGVIAALLLTRVMSGLLYGVSASDPMTFGGISLLLAGVAFLACFVPARRATRVD
                     PMVALRYE"
     gene            complement(4732..4983)
                     /locus_tag="VF240_20010"
     CDS             complement(4732..4983)
                     /locus_tag="VF240_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177554.1"
                     /translation="MGTLWQDLRNSAQMLTSSPAFTHRTSAGDGQRFPVYHPVYKFYR
                     DHNEIFSGLLLCRGGARCARSEQGGETITKRGQRLEIRG"
     gene            complement(5086..>7539)
                     /locus_tag="VF240_20015"
     CDS             complement(5086..>7539)
                     /locus_tag="VF240_20015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177555.1"
                     /translation="VMGTLLQDIRYGFRMMWKKPGFTLVAVLALALGIGANTTVFSVV
                     DALLLRPFNVREPERLFMVYEQNLKAGFERGSVSVSNFLDARADVKSLEHLAAWDNVS
                     LNMSEGDKAERLLGTEVSPEMFAAVDATALLGRVLGAGDAELGKQDVVVISHGLWQRR
                     FGGEESVVGREVNLNGTVHTIVGVMPRGFALPPSSGDVWKPLVFTPQQIERRGAHYLR
                     MMGRLREGATPEQARAELDALAARLQAQYPNSNAGRTFRIETMVESYTRGPRPFLTVL
                     LGAVGFVLLLACANVANLLLVRASSRHKEVAIRMALGASRWRLIRQMLTESVLLALLG
                     GLLGLLVAVWGVDLMRAGIPANLARTMPNWDNVGVNASAFYFALAVSVLTGLLFGLAP
                     ALQATRGGFNESLKEGGRTSGSGMSRNRLRSALVVSEVTLSLVLLIGAGLMIRSFVRL
                     LETEPGFDQSNVMLADIQLAGPRYEGEAPEQEQARGDFYRRLVERVGALAGVESAAVI
                     NIVPLSRNNTNSSYVVVGQPVPPKAQEPMANWRVITPDYLALMKIPLRRGRHLDARDD
                     RTDARPALLVNEALAQKHFPNGDALGQRLNFGGEPEEKNFWEIVGVVGNIKHESLSEV
                     VEPEVYVPYARSAWSGMTIAARTTGDPAQLYAAVEAETAALDRDQPMFNPRTLEQVVA
                     ESLAPQRVTMGMLGVFAVIALLLAAIGIYAVMSYSVAQRTHEIGIRMALGAQPTSILK
                     MIVGQGMLLAGIGIAIGLLAAFFLMQGMTRILYGVSATDPATFVGISLALALVALAAN
                     FFPARRATKVDPMVALRYE"
BASE COUNT         1288 a         2492 c         2576 g         1183 t
ORIGIN      
        1 gccatgcgga tgccgatctc gtgcgtgcgc tgcgtcaccg tgtacgacat cacgccgaag
       61 aggcccacgc cggcgagcgc cagcgcgacg gccgcgaaga tggtgagcag gagcatctgg
      121 aagcggctgc gcgcgaccga gtccgagagc aattcctgca tcgtcttgac gctgtagagg
      181 ggctggttcg ggtcaacctg taagacggcc tgacggacgg cattggctaa gcccgccggg
      241 tcgccgcctt cggagcgcac gacgagggcg aggatcggcc ccggcgcctg ttggtaggag
      301 atgtagaact cgggggccgc gtcctcgttc agacggcggt gcttcacgtc gccgaccacg
      361 ccgacgatct gcgtgtcgta gccgggcgcg acgctcggcg tgacgcgctt gcccacgggg
      421 tcttcgccgg ggaaatattt actggcgaag gtctcgttga tgaccatcac tttgggggcg
      481 cgcgcgtcgt ccgtttcggc gatgacgcgg cccctctgga ggggaatgcc gagggcgcgg
      541 aagtagtcgg ggctgacgat gcgcgcgtgc gaggtcggcc gctcgcccgg cgcggccggc
      601 ggccggtcgg ggaatttgag tgaggtctgc cagccgttgc cgctaaaggg catcggctcg
      661 acgccgccca cggccttcac gcccggcagc gccgcgatgc gttcgagcgc ctgactgtag
      721 aaggccgcct gctgctgctc ctcgccgtac ttcgccgagg gcagcgagat tccggccgtc
      781 aaaacattct gcgcttcgaa gcccggcttg accgagcgca actcaaggaa actgcgcacc
      841 agcagacccg cgccgactaa gagcatgatc gagagcgcga cctcggagac gatcaacaaa
      901 gcgcgcaggc ggttgcgccc gaagccctcg gtcgagccgc gcgagccgtc cttgagcgac
      961 tcgttcaagt cgggcttcga ggcttgcagc gccggcgcga gtccgaagac gacgcccgtc
     1021 agcacggaga cgccgagcgt gaagagcagc actcgcgtgt cgagaccgat gtctttgacg
     1081 cgcgggatgt cggcggggat gagcgcgctc agggcgtcaa cgccccacag cgcgagcagc
     1141 agccccagcg cgcccccgat gagcgccagc agcaaacttt cggtcaacat ctgccgcacc
     1201 acccgccagc ggctcgcgcc gagcgccgtc cgaatcgcga tctccttctg ccgcgaggcg
     1261 gcgcgggcga gcaaaaggtt ggcgacgttg gcgcaggcaa tcaacagcac gaagccgacc
     1321 gcgccgagca gcacgtaaag cgcgggccgc acgtcgccga ccaagtcctc gtacatcgag
     1381 acgacgttgg cgccgcggcc cgcgttcgag gtgttgtatt gctcctcaag tcgccccgtg
     1441 acggcctcca tctcggcctg cccctgctcg acggacacgc ccggcttgag gcgcgcgacc
     1501 acgctcaggt aattgttccc gcgctcctta ttggtcgggg tctccgggtt gaacggcgtc
     1561 cagtactgca cctcctcgac atcgaccggg aacttgaagc cctcgggcat gacgccgacg
     1621 accgtggtgc tctcgccgtc gaaaagaatc tgccggccga ccaccgccgg gtcgccgccg
     1681 aagcgacgct cccagatgcc gtgactcagg acgacgacgt tcgagccggg ccgctcctcc
     1741 tcgcgcgtga aggtgcgccc gagcgccggg ctgacgccga gcagcgggaa gaggtcggcc
     1801 gacgcgtaca cgccctcgac cgactcgggc gtgtcgccga agttcacggc cgcgcccgtg
     1861 ctggcgtgcg cggccgtctg cgtgaagacg ctgctctgct cgcgccaatc gacgaagttg
     1921 agatacgagg tcgggtactt gcgggcgttg cgcttcgcgt cggtcgagaa gacgcggacg
     1981 atgcgctcag actccgggta cggcagcgga cgcagcaaca cggcgttgac gaccgagaag
     2041 attgccgtgt tcgcgccgat gcccacggcg agcgccgcaa ccgtaatcag cgttagcagc
     2101 gggcgcttgg cgatcatcct cgcgccgtat cgaacgtcct gccaaaggtt attcatcacg
     2161 aaacctcaga ggtcagaggt cagaggtcag aggtcagtta aaaagccatc tgtttttact
     2221 aacctctaac ctctgacctc cgacctctta ttcataccgc agtgccacca tcggatcgac
     2281 gcgcgtggcg cggcgcgcgg ggacgaagca ggcgaggaag gccacgcccg cgagcagcag
     2341 cgagatgccg ccgaaggtca tcgggtcaga cgccgagacg ccgtagagca gccctgacat
     2401 gacgcgcgtc agaagcagcg ccgcgatgac gccgatgacg actcccaaaa tgacgagcac
     2461 cattccctgc cggacgacca tcccgaggat gtcgccgccg cgcgcgccca gcgccatgcg
     2521 gatgccgatc tcgtgcgtgc gctgcgtgac ggtgtagctc atcacgccgt agaggccgac
     2581 ggccgcgagc agcagggcga cgccggcgaa gatggtgagg agcgtcatgg cgaagcggcg
     2641 ctgccccgtc gagttggcga cgatctcctc catcgtgcgc gtgttgaaga ccggctggtt
     2701 ggggtcggcc gcgagcacct gctggcgcac ctgcccggcc agagcgagcg ggtcgccctt
     2761 cgtccgcacg actatcgtcc cccgcccgac catgcgcggc agaaacggct cgggtatctg
     2821 cgccagcgag aagtagaact gcggatcgac gggcacctcg ccctccagcc cgtagtgctt
     2881 gacgtgctcg acgacgccga cgatctcgta gggcggagcc tccggggggc cgatggtcag
     2941 gcgctggccg atggggtcct cgtcggggaa cgctttcgcg gcgagcgact cgtcgatgac
     3001 gcacacgagc gcactgcctg agcggtcgcg ctcgtcgagg tagcggccct tcttgagacc
     3061 gatgttcatc gcctcgaaat atccggtggc ggtcgtgtac atcacggcca tcatgaactc
     3121 gcgctcgtcg gcgcgggggc ggccatcgac ccagaacgac tgctcgctgg cgccggcaaa
     3181 cggcagcccc gtcgtgaagg cggcggcctc gatgccgggc agcgcgcgga cgcgcgccgt
     3241 cacgtcgtcg atgaagttca tggccttctg cccctcgccg tctttcgccg ccaccgagag
     3301 ccgcatcgtc aggatgccct tcgggtcgaa cccggtatcg accgactgca agcgccagaa
     3361 gcttctgacc atcagccccg cgccgactaa gagcacgagc gcgacggcga cctccgtgac
     3421 gacgagcgcg ttacgcaggc ggtgacgcgc gcccgtcgag ccgcgctcgc cttccttgag
     3481 cgattcgttg aggtctgggc gcgacgcctg aagcgccggg aagagtccga agacgacgcc
     3541 cgtcaggacg gagacgccga gcgtgaaaac gagcacgcgc gtgtccacgc cggcttcgct
     3601 gagacgcggg atgttgtcgg gcacggcggc aatgagaagg tctgtgcccc acagcgcaag
     3661 caggagtccg gccgcgccgc ccacgagcga aaggatcacg ctctcggtga gcagttggcg
     3721 cacgatgcgc cagcgccccg cgccgagcgc cgtccgaatc gcaatctctt tctgccgcga
     3781 tgccgagcgc gcaagcaaca ggttggcgac gttggcgcag gcaatcaaca acacgaagcc
     3841 gaccgcgccg agcaagacgt agagcgcggg gcgcacgtcc gacacagtgt tctcgtaaag
     3901 cgactcgatg tggatgcgcc tgtcctgatt cgtcttcggg tacgcctgtt cgagctgcgc
     3961 catcagcgtg tccatcccgg cgcgcgcctg ctccaacgtg acgccgggct tcatgcgccc
     4021 gaggacgtag atgccggggt gtgcgccgcg ctccgcgtac tcgctctcga agaggcccaa
     4081 ggggacgaac acgtccgtgt ccgtgccgta gcgatagtcg ggcggggtga tgccgatgac
     4141 ggtgtagctc tggctgttga gcgtgagttc cttgccgacg atagactggt cgccgccgaa
     4201 gcgacgctgc cagaagccgt ggccgaggat ggccgtgcgg ttggcgcccg tgcggtcttc
     4261 gtcggccgtg aagtcgcgcc ccttgaacgg ctcgacgccg aggacgccga agaactcgtg
     4321 cgcgaccatg cgcccctgca aacgctccgg ctcgcccgtc cccgtcaggt tgaagctgtg
     4381 gaggcggccg gcggcgaccg cgtcgaagac cgtctgctgc tcgcgccagt ccttgaagtt
     4441 cgggtaggcg accgacgtgt ccatcttcgg cgtcttctcc cacatgatcg ccaggcgctc
     4501 cgactccctg tactggagcg gacgcagcaa cacggcgttg accaccgaga agatggccgt
     4561 gttcgcgccg atgccgaggg cgagcgccag gatggcgatg aacgtcacgc ccggcgtctt
     4621 cagcagcatc cgaaagccgt acttcaagtc ttgcagcatc gttcccataa caagctccag
     4681 agaggtcaga ggtcagaggt cagaggtcag ttaaagaaga agaggttttt actaacctct
     4741 gatctctaac ctctgacctc tcttagttat cgtttcgccg ccttgttcgc ttcgggcgca
     4801 cctcgccccg ccccggcaca agaggaggcc ggagaagatt tcgttgtgat cgcgatagaa
     4861 cttgtagacg gggtggtaga ccgggaaccg ctggccgtcg cctgcgctcg tccgatgcgt
     4921 gaacgcgggg ctactcgtca gcatttgcgc cgagttacgc aggtcttgcc agagcgtccc
     4981 catcgaacct ccttcggagg tagtaagcag taagcagtga gcagtagaag tgcaactgac
     5041 attgagcggt cttgctttta ctgcttactg ctcactgctt actgctcact cgtagcgaag
     5101 cgccaccatc ggatcgactt tggtcgcgcg gcgcgcgggg aagaagttcg cggccagggc
     5161 gacgagcgcg agcgcgagcg agatgccgac gaaggtcgcg gggtcggtcg ccgagacgcc
     5221 gtagaggatg cgcgtcatcc cctgcatcag gaagaaggcg gcgagcagtc cgatggcgat
     5281 gccgatcccg gccagcaaca tcccctgccc gacgatcatc ttgaggatgc tggtgggctg
     5341 tgcgccgagc gccatgcgga tgccgatctc gtgcgtgcgt tgcgccacgg agtaggacat
     5401 cacggcgtag atgccgatgg ccgcgagcaa cagcgcgatg acggcgaaga cgccgagcat
     5461 ccccatcgtc acccgctgcg gcgcgagcga ctcggcgacg acctgctcca gcgtccgcgg
     5521 gttgaacatg ggctggtcgc ggtcgagcgc ggccgtctcc gcctcgaccg ccgcgtagag
     5581 ttgcgcgggg tcgcccgtcg tccgcgcggc gatggtcatg cccgaccagg ccgacctggc
     5641 gtagggcacg tagacctccg gctcaaccac ctcgctcaag ctctcgtgct tgatgttgcc
     5701 gacgacgccg acgatctccc agaaattctt ctcctctggt tcgccgccga agttgagccg
     5761 ctggccgagg gcgtcgccgt tggggaagtg cttttgcgcg agcgcctcgt tgacgaggag
     5821 ggcgggccgc gcgtcggtgc ggtcgtcgcg cgcgtcgagg tggcgaccgc gccggagcgg
     5881 aatcttcatc aacgcgaggt agtcgggagt gatgacgcgc cagttggcca tcggctcctg
     5941 cgctttgggc ggcacgggct ggccgacgac gacgtagctg gagttggtgt tgttgcggct
     6001 caaggggacg atgttgatga cggccgcgct ctcgacgccc gcgagcgccc cgacgcgctc
     6061 gacgagccgc cggtagaagt cgccgcgcgc ctgctcctgt tccggcgcct cgccctcgta
     6121 gcgcggcccg gcgagttgga tgtcggccag catcacgttc gactggtcga agcccggctc
     6181 ggtttcgagc aggcggacga aggagcggat catcaatccc gcgccgatga ggagcacgag
     6241 cgagagcgtg acctcggaga cgaccagggc cgagcgcagg cggttgcggc tcatgccgct
     6301 gcccgacgtg cgaccgccct ccttgagcga ctcgttgaag ccgcccctcg tcgcctgaag
     6361 cgccggggcg agtccgaaga ggagacccgt cagtactgag acggcgagcg cgaagtagaa
     6421 ggccgaggcg ttgacgccga cgttgtccca gttcggcatc gtgcgggcga ggttggccgg
     6481 gatgccggcg cgcatgaggt caacgcccca caccgcgacg agcaggccga gcaggccgcc
     6541 gaggagcgcc agcagcaccg actcggtaag catctgtctg atgagccgcc agcgcgacgc
     6601 gcccagcgcc atgcggatgg cgacctcctt gtggcgcgag gaggcgcgca cgagcaggag
     6661 gttggcgacg ttggcgcacg ccagcagcag cacgaagccg accgcgccga gcagcaccgt
     6721 gaggaagggc cgggggccgc gcgtgtaaga ctcgaccatc gtctcaatcc tgaaggtgcg
     6781 gcccgcgttg ctgttggggt actgcgcctg taggcgcgcg gcgagagcgt cgagttcggc
     6841 gcgcgcctgc tcgggcgtcg ccccttcgcg caggcggccc atcatgcgca ggtaatgtgc
     6901 gccgcggcgc tcgatctgct ggggggtgaa gacgagcggc ttccacacgt cgcccgagct
     6961 ggggggcaac gcaaaaccgc gcggcatcac gccgacgatg gtgtgaactg tcccgttgag
     7021 gttcacctcg cggccgacga ccgactcttc gccgccgaag cgccgctgcc agaggccgtg
     7081 gctgatgacg accacgtcct gtttgcccag ctccgcgtcg ccggccccga ggacgcgccc
     7141 cagaagcgcc gtggcgtcca ccgccgcgaa catctccggc gagacctcgg tgccgagcag
     7201 gcgctccgcc ttgtcgccct cgctcatgtt gagcgagacg ttgtcccacg ccgcgaggtg
     7261 ttcgagcgac ttcacgtcgg cgcgcgcgtc gaggaagttc gacaccgaga ccgacccgcg
     7321 ctcgaagccg gccttgaggt tctgctcata gaccatgaac agccgctccg gctcgcgcac
     7381 gttgaacggc cgcagcagca gcgcgtccac cacgctgaag acggtcgtgt tcgcgccgat
     7441 gcccaaagcg agcgccagca ccgccacgag cgtgaagccc ggcttcttcc acatcatccg
     7501 aaagccgtag cgaatgtctt gcagcagtgt tcccatgac
//
LOCUS       DASVES010000479        28140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1121992, whole genome shotgun sequence.
ACCESSION   DASVES010000479 DASVES010000000
VERSION     DASVES010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 28140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121992"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VF240_20020"
     CDS             complement(<1..1529)
                     /locus_tag="VF240_20020"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HEX7177556.1"
                     /translation="MNDKEWTIMIYMAGDNNLSVDMAYAMEQIKAMAGEGPDSPNLFV
                     YYDGNSPSIPTLYCDFSEPGKAKYVRSFMVPDKHYVVPAKHNENAAHGYSVVNFVDWC
                     INKVEVEQNGEITYGRRARRYALIFSGHTLGFQDIGLFKDETSGKSMKMEDLYFTLQR
                     LVLQKKDLKKFARENRLKGEEHERDPKVFLGQPFDILGFDSCVMGMLEVGYQFNYVAK
                     TMIASEGSVPNAGWTYAKILGCLARGDNSDDSGPEKVAEQFVRDFIQSQDAYTVGGVS
                     VDMAAWDLSRFDDLAVVFEELSETLIECFKDEGSRLYRQMERVITLVHWKCQSYMYDQ
                     NVDLGDFCELLDRECGLVADELGGDDVEILKRVQKKCRKVLSELKKAVILSGFSGGTY
                     QYSNGISVFFPWSREGYEVSRKNYESLWFARDATRKRVSWSGFLKKYLYEVALRKFEP
                     PVADVPPGSKYRYRSGIRYDERFHTTTFVGQEDSKLAGQEGSKLAGQEGSKLAGQEGS
                     KL"
     gene            complement(1868..3994)
                     /locus_tag="VF240_20025"
     CDS             complement(1868..3994)
                     /locus_tag="VF240_20025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEX7177557.1"
                     /translation="MKRPSLILTAAALAVLLAVCWSVLAHEGEHHSPGGNGQLDNAPL
                     NAMGLTPCVNGMAGTFPCRDIDLASLLPLSQIGGGTGNDVWGWTDHLTGKEYALLGRS
                     NGISFVDITDPENPVYLGNLPTHNAIDSLWRGLKVYRNHLYVISEAAGHGMQVFDLTQ
                     LRSVAAPPMTFAETAHYNNFGSAHTIAVNEATGFVYVAGSRPDPGHTVGVNTCIGATP
                     TRGGGLHIVDVRNPAAPAFAGCVDNDGYTHETQCVIYRGPDAAHRGREICFSSNEDTL
                     TIVDVTNKSAPVQLSRTTYEGRGYTHQGWLTDDQTHFLLDDELDEQHAQQQQQPVKTK
                     TFIWDVTNIDAPFVRNVFQNQTTSIDHNLYVRGRYVFESNYRSGLRILDASAAPAGVL
                     TESAFFDVYPIDDQALFNGTWANYPFFASGIVIASGIEQGLFVLRPRVAQLPVKIDYS
                     PYFVRQHYRDFLGREPDEAGLDFWNFEVARCVADAGCLDDRRVSVSAAFFISIEFQQT
                     GYLVHRFHAASFPGSLPRFQPFLADLRRVANNVVVGAPGWELQLEANKAAFALEWVGR
                     PEFIAQLPTSMTGEQYVDALFANAAVSPSVDERAAALAAFGAGGEQGRAAALRSVADT
                     GAVYNRHFNPAFVLMQYFGYLRRNPDDAPNTDFSGYQFWLDKLNSHSLPGEDVRQDGV
                     ALGRVRRAEMVRSFLISGEYRGRFGL"
     gene            complement(4207..4473)
                     /locus_tag="VF240_20030"
     CDS             complement(4207..4473)
                     /locus_tag="VF240_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177558.1"
                     /translation="MATYRDPVRVFAPMEVVERLLEIRADAGYTPAIFLRTADDLAQY
                     LDAYGFDVYALMDGMDGEAEFHYGKYRAPIVPGGDQAHCSLTAH"
     gene            4835..6064
                     /locus_tag="VF240_20035"
     CDS             4835..6064
                     /locus_tag="VF240_20035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7177559.1"
                     /translation="MSTQPVTPPQQHVAGATTPASLNFIGVLQRMLAVSDKVSDLIFS
                     PGRPPQIELMGQLQPVPVPGLERLLPAHTASIAKLLIGSQQVAGENLEKLGSADISYS
                     APGLARFRVNIFMQRGTHAIVMRVIPACPPQWSDFNLPVVLKEVADIKNGLVLVTGPT
                     GSGKSTTLAAIIDLVNETKHYHIITIEDPIEFLHSHKNSTIHQRELHSDTPDFARALR
                     AALRQAPKVILVGEMRDRETIEVALEAAETGHLVLSTLHTIDAAKTIDRIIGVFPKNE
                     EKAIRTRLAQTFRFIVSQRLLPRADGEGRVAAIEILKSTARTRDYMERGESEGRSLTD
                     AMDQGELDGMQTFDGVIEKMIREGVVDKEEALHYASNSGNLQLRLADYGGATVPPGNN
                     GRPAAAEQSGSMLDMLE"
     gene            6249..7952
                     /locus_tag="VF240_20040"
     CDS             6249..7952
                     /locus_tag="VF240_20040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7177560.1"
                     /translation="MPSAAATASAGGIHTQRPSSDPAGFVSFAPPASGEVRVENQRGG
                     VDVEVWDEAFVGVASSGVVVGEQKGRRVGRRRQPSSPVRVERSETLLSIHVPRAAGTT
                     ASAAAPVDLKLRLPRSARARIYTSDGPIEVRGAPAQLSAQTISGAIRLMLSTDADADI
                     TAHSLNGKVSVREGVGAEARLKSEERQKFQTRVGGGASVVRLFSGRGQINLELSGTPD
                     AASVEERTPLPSPPARNERPVEERPAERRAPALVGARGEALRPAPSATPEAPQEVDED
                     EVIRVDSELVTVNISVVDRARGRGLTGLVQDDFQLAEDGVLQSIEHFEAAAAPFDLVL
                     LIDLSGSTGKVTDLIRAAAVRFIDAARAQDRIAVLAFAGATNVVSPLTTERERLRASV
                     RAMGRPDGHTRVYDSTAAALDFLEKNAGSQRRRAVVLLSDGLDSTMPNVTGIGSQLPY
                     EELRSRVQEFEGLFYSIWTSTEYEAFSPLDIQPETFDLAHDRLQELAEAGGGVFYDVE
                     RLEDLAGAYERVVEDLGTVYSLSYRPTNKTRDGRWRAIRVRLPGHPQAVARGKRGYNA
                     N"
     gene            complement(8045..8620)
                     /locus_tag="VF240_20045"
     CDS             complement(8045..8620)
                     /locus_tag="VF240_20045"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7177561.1"
                     /translation="MVSRIHTSPLIKTYTLEEFWELPDPPDRSKLELIAGVIYMSPPP
                     EYTHDHVVTRLVRLLTRHMDATDDKGTLFVPRAAIWTGARTYLEPDLFYVSSELAARL
                     DPARRTTADLVIEVISPGSAIYDRNTKADTYGALGIRELWLVDETAETVEVRRRVEGG
                     FDEGQFFEKGERVRSDVFPGLQLPVESIFKD"
     gene            8841..10202
                     /locus_tag="VF240_20050"
     CDS             8841..10202
                     /locus_tag="VF240_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177562.1"
                     /translation="MRAREGRLILYFVIAAAALLLAACGKNRAGQGGGGGGGSVAAPA
                     AGVLPVAEATAPKRTLAPLPARELTPELRSARARAMADASRLRGLAWRGEVGMTPLSA
                     WEFGTRAAELADALGAEDLQTLGRLAVAGGILPPGTDLAATAVGFTAATAGAFYSPLD
                     RQVIVRAEGTGSAAAKKHALLTHEFVHALQDQHFDLLGLLLAKPYNFDRAEAAFALVE
                     GDATVVQRRVEQGDAFARRTLEEVARAEDARFDSYRREVGWLFPALLTETFVFRYRDG
                     ARFVETLRRGRPAMGADELFRRPPVSTEQVLHPEKYLAGEAPREVSLNVEGLTNDGWR
                     ASGSTPLGEIGVRGLLLGRVAAARATRAAAGWGGDLAGLFEREGHAPLFVWKSVWDTA
                     ADAREFFAAYGAPHRRAGASTQTREEVWREGAALTLVRLEGDAVLVIRGAEDDVRAAA
                     RMF"
     gene            10334..11446
                     /locus_tag="VF240_20055"
     CDS             10334..11446
                     /locus_tag="VF240_20055"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX7177563.1"
                     /translation="MKAAFRSAANSVFALTLVVLCAGFTAADAQGNRDLRVVSARAGG
                     VNYAIGDVKLRRRGQTRWQAFGGDSTTDLDSGDSLKTGATGFAEVLLNPGSYLRLGAD
                     SEFVLTDSSLDDLSLQLVRGSAVIEATGYDRMGLLIAVDTPQSRVSIIKSGIYRFNVL
                     ASGQTEVAVRKGRVSVGEEPVSLIVKEGNVARVDRRATEVVKLDKKQRDALDAWSKGR
                     ADELAEANRKITRRQANTLLASLRFNANYGYSSGAGVWYFDTRRNCYTFVPFAAGWRS
                     PYGGWYDNMVWGHPMPCGGCSGGRNPYVFTSGGQYGNPGATSGGGYPGGTTPGGSSGY
                     PGGGSGVNPPPTPVVRERTPIDYGPVTRKMTREVEP"
     gene            complement(11527..13026)
                     /locus_tag="VF240_20060"
     CDS             complement(11527..13026)
                     /locus_tag="VF240_20060"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7177564.1"
                     /translation="MSQHDVVVVGGGIGGLTTAALLAARGLDVCLFERESRAGGCAAA
                     FEHMGYAFEQGAGLYACWQPGEIHERVFAELPLGAPEVRRAAPAYVVRLPDGAEVRVG
                     GEREEFDETLRAAFPECAAASLEFYREARAIGDALRRSAARVPALVTATRIERVKLIA
                     QEARLSARILGSMGHTAAEHLRDTSARFRRFIDAQLCVFAQRPSDECAYLYAAVALSH
                     AARGMYELRGGAQALADALVESIKMSGGTVRLSATVLRLARDASGRAAGVDLLSGERV
                     EARRAVVSNLTVWDTYGKLVGLADTSTDVRARMKRLRGWGAYLIFIALDERAAARLPA
                     THVLALTDFQEGARLDPEESLLMLGAAPGWDARAPEGFRAATVCTHTDAEQWFAFHDD
                     EAAHEEQDAAALEKCWARLHRALPELGAGAEVIETATPRTCYERTRRRLGMVGGTGQS
                     LDVFGAGALTHRTSVPRLYMVGDTVFPGQGVAAVTHSALIAANEIAPPR"
     gene            complement(13046..13936)
                     /locus_tag="VF240_20065"
     CDS             complement(13046..13936)
                     /locus_tag="VF240_20065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7177565.1"
                     /translation="MSTPLEPVADNPFADFAPDAAAPAAGLIDPDNPPWGVLAGFSVW
                     LASIVLMVVAYLLVVIPYVLITQGISASGQLRESLMSDPNMMLLSVASVVPSHLATLL
                     LVWAVVTNFGKRPFFRTIGWGWSPRFGLWTSAALAVLLLVLGGVLMYFLGRDVKTPFD
                     EMLESSVQARFATAFLATATAPLVEELVYRGVLYPALRRAMGMFAAVVAVATLFTVVH
                     VSQYYNNPAVIATVGGLAFALTYVRARTGRLLPCFVIHLVFNGIQCAWLVLEYFYPLP
                     QQQPTETQVGLVVALLPWLL"
     gene            complement(14045..14875)
                     /locus_tag="VF240_20070"
     CDS             complement(14045..14875)
                     /locus_tag="VF240_20070"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7177566.1"
                     /translation="MRQAETRTKSDARGEEIHSPVAAVDVPRTEGARVPTLFNRLHTF
                     VAWPLVALYTVVMGTLSLALSVFDRRGRLQHWCARTWSRMIARTALLDVRVHGADNVR
                     PGASYVFLSTHQSWMDIPVMLGYLPSQLRIAAKREVFLIPFLGWHMTRAGHIPVNRGS
                     TTEAVESLRRASASLRDGVSAFLFPEGTRTRDGSLQPLKKGGFRLAIESGLPIIPVAI
                     VGTRRAMPRDSAVLRAGPVELYIDAPLQTDGLTAEDLPALVAAVRAAMERHFYKGRES
                     "
     gene            complement(15100..15678)
                     /locus_tag="VF240_20075"
     CDS             complement(15100..15678)
                     /locus_tag="VF240_20075"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7177567.1"
                     /translation="MDIVDKESPEVLHSEELFRGRIFDVTRDTVREGEKIYVRECVQH
                     PGGASVVAVFDDRTVALVRQYRHPARRYLLELPAGKLEAGERPEDCAARELIEEVGFS
                     AGRLEPLSEFYPTPGFCAERLWVFLATALTEATQQLEDDELVEVVRLPLRRALSMIAS
                     REIEDAKTIIGLILAAQHFGISTNDVAETAAL"
     gene            complement(15778..16233)
                     /locus_tag="VF240_20080"
     CDS             complement(15778..16233)
                     /locus_tag="VF240_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177568.1"
                     /translation="MTRQGRILIILALAGLAVLAALVAVLLNTETDAKRRRAAATYEA
                     TAAQHLKNLAAAEQNYLDNFGEYATFDQLLAAGILLNKEFAGDAPAVDGYVFRLRLTP
                     RAGERPPSYSINADPHAAGGERHLYLDSEVIGIRYSDGRPATAADRQRE"
     gene            complement(16271..17572)
                     /locus_tag="VF240_20085"
     CDS             complement(16271..17572)
                     /locus_tag="VF240_20085"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX7177569.1"
                     /translation="MLYDESLRYLLSLGHETLAMKLGLEGVRRLLALLGDPQKSYPAI
                     QIAGTNGKGSTAAMLDAVCRAAHIRAGLYTSPHLVSITERVRVGGVEVSREEFARAAT
                     RVRAAAEEVGRMTGAVPTFFEQVTAAALLVFKETGVRVAILETGLGGRLDATTAAEAG
                     VVAITPVALDHQEHLGCTLEEIAAEKAAIIWPGVAAAVIAPQEPAAAAVILRRCRECG
                     VEPRLVAPSDARVEEICADGRLRVSFTTGRGTYGHVRLNLRGRHQLVNAATAVRLAEI
                     LAERGFEITPENVVAGLESAEHPGRLELREGSPAVLFDGAHNPAGARALRAYLDEFAR
                     APVTLVFGAMRDKEVEEIGRALFPAARRLVLTQADNARSAPVEDLVRAIPRDTDLSTI
                     SLAPAPRDALAIARAVTPPEGTICVTGSLYLVGEMTRLLGD"
     gene            complement(17723..18718)
                     /gene="accD"
                     /locus_tag="VF240_20090"
     CDS             complement(17723..18718)
                     /gene="accD"
                     /locus_tag="VF240_20090"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX7177570.1"
                     /translation="MSKWFRKKNQRIEPVPADERRVKTEGVFQKCPECDAALYTRELE
                     ENLHVCHHCSHHFRMGARARLAMLYDEGKFEETDGEVISSDPLNFVDTKPYKERLEQA
                     RRATGLPEAVINARGQMGGMPVLCAAMDFSFLGGSMGSAVGEKITRLIERAIFERSAV
                     VIVSASGGARMQEGILSLMQMAKVAAALALLSEQHLPYVSVLTDPTTGGVTASFAMLG
                     DVIIAEPKALIGFAGPRVIEQTIRQKLPKDFQRSEFLVEHGMLDAVVDRRQMRDAVIR
                     LLQFMYNPEMERPDIPVELLPEATFTPQTHQLELPGNNGAPEEGEVQSSKFKVQS"
     gene            complement(18832..19854)
                     /locus_tag="VF240_20095"
     CDS             complement(18832..19854)
                     /locus_tag="VF240_20095"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX7177571.1"
                     /translation="MLSQVVELIEAKRRFAITSHVRPDGDALGSSLGLYWLLRSLDKE
                     AEVIMRDAAPHAYHKLPGAELIRVTPSVDKPYDAVFVIECSDITRPGLERLEEQLVVN
                     IDHHSTTSLFGTINWIDSTASAVGEMIYNLCKAIGVRVTREIAECVYTALITDTGSFH
                     YANTTERTFKVASELVRAGVKPAKVSQAVFGSYAWGKIRLTSLVLSTVNRDPTGRVAW
                     LRQTLDMQTQAGASEEDADGFVNYPLSCGEVEAVAFLKETQPGLYRVSLRSKGDVNVA
                     RIAERFGGGGHRNAAGCTLGGDWDETEEMIVSHLIEAVERRSNGAPPPLPGGGEPVAE
                     MSGVGK"
     gene            complement(19935..20348)
                     /gene="rbfA"
                     /locus_tag="VF240_20100"
     CDS             complement(19935..20348)
                     /gene="rbfA"
                     /locus_tag="VF240_20100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7177572.1"
                     /translation="MRRPQQLAELIREELSQIVGYELEDPRVETATVTDVRVTENLRD
                     ARVFVLVEGTDDEKTLALKALTKAAPYVRRQLGLALNLKFTPELHFVRDTVEEKAARV
                     EELLAQVKQDSGSSSESVAAAGDGPAETEEKGENK"
     gene            complement(20430..23270)
                     /gene="infB"
                     /locus_tag="VF240_20105"
     CDS             complement(20430..23270)
                     /gene="infB"
                     /locus_tag="VF240_20105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7177573.1"
                     /translation="MTTALSKIRIYDLAKELKMESKRLIEEVRREGVDVSVPSNTISK
                     ELAEKIRNKYFPKKEVHAPRSIRVVKKAARPVETETPAADDEHSPQHAPVTMEPAPES
                     TPAPPTLEAHAPEVSEPPAAGGGGGARLVPARLVKRPAATRAERPEEAAVAEAPAEAQ
                     EVSYVEPEAAAVETEEFETPAPAAVYATEETEMETVPAAAVPSGPSATPAPARQVRAL
                     RPTTAALNAGIRHGERAPDVAPAVVTPPAAARARSGGGGRDERPRNGRGGRDEALRGT
                     PGESATPQTTYIPPADARRPGGRPGSRRGTGRLSGGPRERGGRYEKDFVLPPKALTPE
                     QRFAQNLALAGETKQVSLVEGSTVKEFAEKLGIKPKEVVTLLMQRGVFATINQPLDDE
                     VAVDLGKRFGFEVSFMPYEDMVTGQELEDILGGENIDDYVEEARAPVVTVMGHVDHGK
                     TSLLDAIRTAKVAEGEAGGITQHIGAYSVQVASADDPLQLRRVVFLDTPGHEAFTLMR
                     ARGAKATDVVVLVVAADDGVMPQTIEAVEHAKAAGVPIVVAINKIDKPDANPDRVKQG
                     LAGLGLQPVDWGGETEMVAVSAKKRENLETLLETILLTADILNLKASPTRLASGVVLE
                     AKLDRGRGAVATVLVQQGTLKVHDPFIVGQIFGKVRAMFNDRGELVTEVGPATPVEVL
                     GMQGVPQAGDTFQVVADITKAQSISQHRQMISRQAALVQTTKRGIEALGQQETKELLI
                     VVKADVQGSVEVLKSTLQKLSTEKVKVKVIRSGVGAITESDVLLASATQASASSSAVV
                     IIGFNVRPEVRAAELGKHEHVDVRLHSIIYKVEEEIRAAMLGMLDAIETERIIGNASV
                     QEVFRVPKIGAVAGCRVTSGVISRKARARLIRDGVVAWEGQIASLRRFKDDAAEVREG
                     FECGIGLENFNDIKVNDQIEAYVIESVAATEL"
     gene            complement(23454..25481)
                     /gene="nusA"
                     /locus_tag="VF240_20110"
     CDS             complement(23454..25481)
                     /gene="nusA"
                     /locus_tag="VF240_20110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7177574.1"
                     /translation="MSASIPQSIDALCKEHGIDRDIVIDAMKEAVKAAARKQFKEEIQ
                     VEWTTESGLEIFAAKVVVEEVENPARELSLDEAKELAGDEVEVGDELQLPMPMEEMGR
                     IAAQTAKQILFQKVRDAVRQNVYEQYIDKVGDLVNGYIKRFERGDIIVDLGNVEALLG
                     RKEQSRGEQWNQGERIRVVIDDVSKESKGPQVIVSRTSPELLKRLFEMEVPEIYDSTV
                     VIKSAVREPGERAKIAVASTERDVDPVGACVGMKGSRVQAIIRELRGEKIDIIEWSDE
                     PSVFAANSLSPAKVNQVRITDIERRQMEVIVNEDQLSLAIGKRGQNVRLATKLVGWNI
                     DIRSEEEIKREVAAQMGAMIASGEAVPLERLDGINPAMIDALALRGIEDIETLATTSI
                     DDLTEYLDLSIDDAQRIISSAQSIVSAAREAGQQSDEEAAAAEAGDAAATDETAAVET
                     GGDGSETDTDAGSETAAEASPDAEASTEVSEGGDDETGAVEDAGETLSDGGAEETRGT
                     VEQHAASFEFDPDAPAREVEPSEAMIAEGYDAAVAGGTPFTADPDHLTGESGEPVALS
                     ESDPMSADELLLQGGGRDLRADTITPAPDITSSEAAVLETSGAAMELERTEAEEEDDS
                     PLSPAADLGGEASAPEVAQSDVPSDSERVEASEPDEGVAPSEKPDDASGNA"
     gene            complement(25569..26111)
                     /gene="rimP"
                     /locus_tag="VF240_20115"
     CDS             complement(25569..26111)
                     /gene="rimP"
                     /locus_tag="VF240_20115"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7177575.1"
                     /translation="MTDGDSVEGRVREIATRAAEERGLELVHVEMARTGRLRTLRVFI
                     DRPDGVTHEDCSEVSHHMSTVLDVEDFIHEPYNLEVSSPGLERGLYRREDYERFAGRL
                     ARMKSREAVDGQRNFRGRIVGVEGDKVVFEDVTTGRVLVSVASIVKANLEIDVEEEFR
                     RAEQRERELRSAQAEESAES"
     gene            complement(26372..26794)
                     /locus_tag="VF240_20120"
     CDS             complement(26372..26794)
                     /locus_tag="VF240_20120"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7177576.1"
                     /translation="MSDMTAAMKARDAARTSTLRMVKAALQNREIEKGGELTDEETTK
                     SLLSLVKQRRDSVEQYEKAGRNELAEKELAEIAVIDEYLPRAASREEIEGAVAAVISE
                     TGATSMKEMGAVMKAVQGRLGGAADGRIVSEIVKARLQ"
     gene            complement(27159..>28140)
                     /locus_tag="VF240_20125"
     CDS             complement(27159..>28140)
                     /locus_tag="VF240_20125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7177577.1"
                     /translation="AATPNGVQWTLYFDASTNLLSKQEIVVSDNLAGDTTHEWAFPGY
                     RTVGRVKVPTGRVTRRAGELIEEVTYDDVQFNTKPAESAFAKPEGHEELPTPGPAPTR
                     ETKLADGVHLFESGANSLVVEFSDHLLVVEPYAGGRGSRATITKLKEMFPAKPIRYVV
                     VTHFHDDHSGGLRPYVAAGATIVTTAANQKFFERMAASTFTVTPDDQSRALRKPAFEF
                     VQNGKRVFTDGKQSVEVIDVGPNPHAREMLVAYLPKERLVFQGDLVSLPNSGKYMPTT
                     IIEATTHFLETITRLGLDVERIASVHGPSTTLRELREAAGRSRPQSSSQE"
BASE COUNT         4577 a         9229 c         9414 g         4920 t
ORIGIN      
        1 agcttggaac cctcttgacc ggctagtttc gaaccttctt gtccggcgag cttggagcct
       61 tcttgacctg ccagctttga gtcttcctga cctacaaacg tcgttgtgtg gaaccgctca
      121 tcatatctaa tgcccgaacg atacctgtac ttcgatccgg gagggacatc tgccacaggc
      181 ggctcaaatt tgcgcagggc gacttcgtaa agatacttct taaggaaacc gctccatgat
      241 actcgttttc gtgttgcgtc cctggcaaac cacaggctct cgtagttctt ccgcgatacc
      301 tcgtagccct cgcgcgacca cgggaaaaac accgagattc cgttcgagta ctggtaagtt
      361 cctccactaa atccactcag gatcacggcc ttttttagct cgctcaatac ctttcgacat
      421 tttttctgca cccttttcag aatctccaca tcgtcgccgc caagctcgtc cgcgaccaga
      481 ccacattccc gatcaagcag ctcgcaaaaa tccccgagat cgacattctg gtcgtacatg
      541 tacgactgac atttccagtg gaccagggta attactcgtt ccatctgcct gtagaggcgg
      601 cttccctcgt ccttaaagca ttcaatgaga gtctcggata actcctcgaa cacgacggcc
      661 aggtcgtcaa accggctgag gtcccatgcg gccatatcga ctgagacgcc gccgaccgtg
      721 taagcgtcct ggctctggat gaaatccctg acgaattgtt ccgccacttt ttcggggccg
      781 ctatcgtcgc tgttatcgcc gcgggctaaa catccgagta ttttggcata cgtccagccg
      841 gcgttcggaa cgctgccctc ggaggcgatc atcgtcttag ccacatagtt gaactgatag
      901 ccgacttcaa gcatgcccat cacgcagcta tcgaatccca aaatgtcgaa cggctggccg
      961 agaaatactt ttgggtcgcg ctcatgttcc tcacctttca acctattttc cctcgcgaat
     1021 ttttttaaat cttttttctg gaggacaagc cgttggagcg taaaataaag gtcttccatt
     1081 ttcatcgact tacctgaggt ctcgtctttg aataagccga tgtcctgaaa gccgagagtg
     1141 tgtccggaaa atatcagcgc gtacctacgg gcacggcggc cataggtgat ctcgccgttc
     1201 tgttcgacct cgaccttgtt gatgcaccag tctacaaaat tcaccaccga gtatccgtgt
     1261 gcggcgttct cattatgttt agccggaacc acatagtgtt tgtcgggcac cataaatgag
     1321 cggacatact tcgccttgcc cggttcggag aaatcacaat agagggtcgg gatcgacggc
     1381 gagttgccgt cgtagtagac gaacaggttc gggctatcag gcccttcgcc ggccattgcc
     1441 ttgatctgtt ccatggcata ggccatatcg acggacaggt tattatcgcc ggccatatag
     1501 atcatgatgg tccattcttt atcgttcatt gtttccggcc tcctgccggt agtcgatggc
     1561 tggacaggcg agggccctgt actatcgcgg atgggtctac gtgcggcgag gggtgctggc
     1621 gaagctgaag gcgaacctcg gattgtggcg gcgtaagata tttgaatatg gccgccaagt
     1681 caacagatgt cgagcgccgt cggagggcat aactgccgtc gccaaggatg cgggagaacc
     1741 ttgtgtcctg gattctgtgc gaaataatca acgcatactc gcgtgggtac tatgtaccca
     1801 cgcgagtatg ccccctcgta ccacgccgac gctgatcgcg ttccccgatc tcagtgtcgt
     1861 gtcgccctta cagcccgaag cgcccgcggt actcgcctga gatgaggaac gatctgacca
     1921 tctccgcgcg ccgcacgcgt cccagcgcca cgccgtcctg acgaacgtcc tcgcccggca
     1981 ggctgtgcga gttgagcttg tcgagccaga actgataacc gctgaagtct gtgttcggcg
     2041 cgtcgtcggg gttgcggcgg aggtaaccga aatattgcat cagcacgaag gccgggttga
     2101 agtgccggtt gtagaccgcg ccggtgtcgg cgacgctccg cagggccgcg gcgcgcccct
     2161 gctccccgcc cgcgccgaag gcggcgagcg ccgcggcgcg ctcatccacc gaagggctga
     2221 cggcggcgtt ggcgaagagc gcgtccacgt actgctcacc cgtcatgctt gtaggtagct
     2281 gcgcgatgaa ctcgggccgc ccgacccact ccagggcgaa ggccgccttg ttcgcttcga
     2341 gttgtagttc ccagccgggc gcgcccacca ccacattgtt cgcgacgcgg cgcaggtcgg
     2401 cgaggaaggg ctggaagcgc ggcaggcttc cggggaatga agcggcgtgg aagcggtgga
     2461 cgagatagcc ggtctgctgg aactcgatgg agatgaagaa ggcggccgag acgctgacgc
     2521 ggcggtcgtc gaggcaaccg gcgtcggcga cgcagcgggc gacctcgaaa ttccagaagt
     2581 ccagccccgc ctcgtccggc tcgcggccga ggaagtcgcg gtagtgttgt cggacgaagt
     2641 agggcgagta gtcgatcttc acgggtagct gcgcgacgcg cgggcgcagc acgaacaaac
     2701 cctgctcgat gccgctcgcg atgacgatgc cgcttgcgaa gaaggggtag ttggcccatg
     2761 tgccgttgaa gagcgcctga tcatcgatgg ggtaaacgtc gaagaaggcg gactcggtga
     2821 gcactccggc cggcgcggcg ctcgcgtcga ggatgcgcag cccgctccgg tagttcgact
     2881 cgaagacgta tcggccgcga acgtagaggt tgtggtcaat cgacgtggtc tggttctgga
     2941 agacatttct gacgaacggc gcgtcgatgt tggtcacgtc ccagatgaag gtcttggtct
     3001 tgacgggttg ttgctgctgc tgcgcgtgct gctcatcaag ctcgtcgtcg agcagaaagt
     3061 gtgtctggtc gtcggtgagc cagccctgat gcgtgtagcc gcgcccctcg taggtggtgc
     3121 gcgatagttg gacgggcgcg ctcttgttcg tcacgtcaac gatggtgagc gtgtcctcgt
     3181 tcgagctgaa gcagatttcg cggccgcggt gcgccgcgtc ggggccgcgg tagatgacgc
     3241 actgcgtctc gtgcgtgtag ccgtcgttgt ccacacagcc ggcgaaggcc ggggccgcgg
     3301 ggtttctaac atcgacgatg tgaagcccgc cgccgcgtgt gggcgtggct ccgatgcacg
     3361 tgttcacgcc gacggtgtgg ccggggtcgg ggcggctccc ggcgacgtag acgaagccgg
     3421 tcgcctcgtt gacggcgatg gtgtgcgcgc tcccgaaatt gttgtagtgc gccgtctcgg
     3481 cgaaggtcat gggcggggcg gcgaccgagc gcaactgcgt caggtcgaag acctgcatgc
     3541 cgtggcccgc ggcctcgctg atgacgtaga ggtggttgcg gtagaccttc aggccgcgcc
     3601 agagcgaatc gatggcgttg tgcgtgggca ggttgccgag gtagacgggg ttctccgggt
     3661 cggtgatgtc cacgaaggag atgccgttgg agcggcccaa gagcgcatac tctttgccgg
     3721 tgagatggtc ggtccagccc cacacgtcgt tccccgtgcc gccgccgatt tgcgagagcg
     3781 ggagaagcga ggcgaggtcg atgtcgcggc aggggaacgt gccggccatc ccgttgacgc
     3841 agggcgtgag gcccatcgcg ttgagcggcg cgttgtcgag ctggccgttg ccgccgggac
     3901 tgtggtgctc gccttcgtgc gcgagcacag accagcagac cgcgaggagg acggcgagcg
     3961 ccgccgcagt aagaatcaac gagggtcttt tcatctgaaa ccttcctgag gtgggttaga
     4021 acttgagccg gggagccgcg cgcgtcattc tacactacgg cgggccggtt ttcgcacacg
     4081 gcccggtggg gtcgcgccgc gcgggcgcgc gggaaacctt cgagccatat aaagacgagg
     4141 cgggagagag cgttttacag ccatttcccc cgcctcgcgc gttctcgccg accgaagccg
     4201 gtgtgtctag tgcgccgtca gcgaacagtg cgcctgatcg ccgccgggga cgatgggcgc
     4261 gcggtacttg ccgtagtgaa actccgcctc gccgtccatg ccgtccatca aggcgtaaac
     4321 gtcgaagccg taggcgtcga ggtactgagc caggtcatcc gccgtgcgga ggaagattgc
     4381 cggtgtgtag cccgcatcgg cgcggatttc cagcaggcgc tcgacgactt ccatcggtgc
     4441 gaacactctc acagggtctc tgtaagtagc catcttgaac aagcgcctcc tttccgttcc
     4501 aactttgcgg gggcgccacc tcgcggaagc gtcgcgccgg ggagtcccgc gccttgcacc
     4561 attgtgatgt acgcccaaat gcgcgcggtt gtttgcgcgc ccgctttatt ttcggtgccg
     4621 aagtgtttac aaaagctttg ccaaccggcg cgccgatgag gtacattaaa ttacggcccc
     4681 ggaagggaga ccgcccgctc ctcgcgccca cgtcttcgga acacctcatg tcctaacgtc
     4741 tcaatcagga aaacccggca aaatcaggag aagcgccgcc cgcgcagcca ccgcgccctc
     4801 cgagcgcctc ccgccacccc acggtaattc gcttatgagc acacagcccg ttactccccc
     4861 acagcagcac gtcgccggag cgacgacgcc cgcgtcgctc aacttcatag gcgtcctcca
     4921 gcgcatgctc gccgtctccg acaaggtgag cgacctcatt ttctcgcccg gccgcccgcc
     4981 gcagatcgag ttgatgggcc agctccagcc cgtgcccgtc cccggcctcg aaagactcct
     5041 gcccgcccac acagcctcca tcgccaagct gcttatcggc agccagcagg tcgccggcga
     5101 gaatctggag aagctcggct cggccgacat ctcctacagc gcgccgggcc tcgcgcgctt
     5161 ccgcgtcaat atcttcatgc agcgcggcac ccacgccatc gtcatgcgcg tcatccccgc
     5221 gtgcccgccg cagtggagcg acttcaatct gcccgtcgtc ctcaaggagg tcgccgacat
     5281 caagaacggc ctcgtgctcg tcaccggccc gaccggctcc ggcaagtcaa cgactctggc
     5341 cgccatcatc gacctcgtca acgagacgaa gcactaccac atcatcacca tcgaagaccc
     5401 gattgagttc ctccactcac acaagaactc gaccatccac cagcgcgagc tgcactccga
     5461 cacgcccgac ttcgcccgtg ccctccgggc cgcgttgcgt caggcgccga aagtcatcct
     5521 cgtcggcgag atgcgcgacc gtgagaccat cgaggtcgct ttggaggcgg ccgagaccgg
     5581 gcacctcgtg ctctcgaccc tgcacaccat cgacgccgcc aagaccatcg accgcatcat
     5641 cggcgtcttc cccaagaacg aggagaaggc catccgcacg cgcctcgccc agaccttccg
     5701 cttcatcgtc tcgcagcggc tgctgccgcg ggccgacggc gaggggcgcg tcgccgccat
     5761 cgaaatcctg aagtccacgg cccgcacgcg cgactacatg gagcgcggcg agagtgaggg
     5821 tcgctcgctc accgacgcga tggatcaggg cgagcttgac ggcatgcaga ccttcgacgg
     5881 cgtcatcgag aagatgatcc gcgaaggcgt cgttgacaag gaagaggccc tgcactacgc
     5941 ctcgaactcc ggcaacctcc aactgcgact cgccgactac ggcggcgcga ccgtgccgcc
     6001 cggcaacaac ggtcgcccgg ccgcggccga gcagtccggc tcgatgctcg acatgcttga
     6061 gtgaaggcgt gcgctcgcgt tatcttcatg gcgcccgacg gcggggccag cccaagtcgc
     6121 actttcgcgg caaccgcccg acccctcgtc ggacatcaaa ccgcagggga cggatgcgag
     6181 cgccgcttac aaacatcggg catcactgca caagggcgac acttctcgcg ctggccttga
     6241 cgctcgccat gccaagcgcc gccgccacag cctccgccgg cggaatccac acacagcgtc
     6301 catcttccga ccccgccggg ttcgtgtcct tcgcgccccc cgcgtcgggt gaggtgcgcg
     6361 tcgagaacca gcgcgggggc gtggacgttg aggtctggga cgaggcgttc gtcggcgtgg
     6421 cgtcgtccgg cgtcgtcgtt ggcgaacaga agggcaggcg tgtgggacga cggcggcaac
     6481 cgtcgtcgcc cgtccgcgtc gagcggtctg aaactctact cagcatccac gtcccgcgcg
     6541 cggcggggac gacggcctcg gccgccgcgc ccgtcgatct gaagctccgc ctgccgcgct
     6601 ccgcacgcgc cagaatctac acctccgacg gcccgatcga ggtgcgcgga gcgcccgcgc
     6661 agctctcggc gcagaccatc tcgggcgcca tccgcctgat gctcagcacc gacgccgacg
     6721 ccgacatcac cgcgcactcc ctgaacggga aggtgtccgt ccgggaaggc gtcggggcgg
     6781 aggcgcgtct caaatctgag gagcggcaga agtttcagac gcgagtcggc gggggcgcga
     6841 gcgtcgtgcg tctcttctcg ggccgcgggc agatcaacct cgaactttcg gggacgcccg
     6901 acgccgcgtc cgtggaagag aggacaccgc tgccttcgcc gcccgcgagg aatgaaaggc
     6961 ccgtggaaga acggccggcg gagcgacgcg cgcccgcgct cgtaggcgcg cgcggcgagg
     7021 cgttgaggcc cgccccgtcg gccacgccgg aggcgccgca ggaagttgac gaggacgaag
     7081 tgatccgcgt cgatagcgag ctggtcaccg tcaacatcag cgtggttgac cgcgcgcgcg
     7141 ggcgcggttt gacggggctt gtgcaggacg actttcaact cgcggaggac ggtgtcctcc
     7201 agagcatcga gcacttcgag gccgccgccg cgccgttcga cctcgtcctc ctgatcgacc
     7261 tctccggctc caccggcaaa gtcaccgacc tcatccgcgc cgccgccgtg cgcttcatcg
     7321 acgccgcgcg cgcacaggat cgcatcgccg tcctcgcctt cgccggcgcg accaacgtcg
     7381 tctcgccgct gacgaccgag cgcgagcgtc tgcgcgcgtc ggtgcgcgcg atggggcggc
     7441 ccgacggcca cacgcgggtc tacgattcga cggccgccgc gctcgacttc cttgagaaga
     7501 atgccggcag ccagcgacgc cgcgccgtcg ttctgttgag cgacggactc gacagcacca
     7561 tgcccaacgt caccgggatt ggctcgcaac tcccctacga ggagctacgc agccgcgtgc
     7621 aggagttcga ggggctgttc tattccatct ggacgagcac agagtatgag gcgttcagcc
     7681 cactcgacat ccagccggag acgttcgacc tcgcgcacga ccgcctgcaa gagctggccg
     7741 aggccggcgg cggcgtcttc tatgacgtgg agcgcctcga agacctcgcc ggcgcatacg
     7801 aacgcgtcgt cgaagacctc ggcacggtct atagcctctc ctaccgcccc acgaacaaga
     7861 cgcgcgacgg ccgctggcgc gccatccgcg tccgcctccc cggccacccg caggccgtcg
     7921 cccgcggcaa gcgcggctac aacgcgaact aaaacaaaca gtaccgcgag cggtagcgag
     7981 cgggtctgct gtcataacca cacccgctcg ttaccgctcg cggtactgtt tgttttaaat
     8041 cgggtcagtc cttgaagatc gattcgacgg gaagctgaag gccggggaaa acgtccgaac
     8101 gcacgcgctc gcccttctca aaaaactgtc cttcgtcaaa gccgccctcc actctccgcc
     8161 gcacttcgac cgtctctgca gtctcatcaa ccagccacag ctcgcggatg ccgagcgcgc
     8221 cgtaggtgtc ggccttcgtg ttgcggtcgt agatggccga gccgggggag atgacttcga
     8281 tgacgaggtc ggccgtcgtg cgtcgggccg ggtcgaggcg cgcggcgagt tccgaggaga
     8341 cgtagaacag gtctggctca aggtacgtcc gcgcgcccgt ccagatggcc gcgcggggga
     8401 cgaataaggt tcccttgtcg tccgtcgcat ccatgtgacg agtgagcagg cggacgagcc
     8461 ttgtaacgac gtggtcgtga gtgtattcag gcggtgggga catgtagatc actccggcga
     8521 tgagttcgag cttggagcgg tcgggcgggt cgggcaactc ccaaaactct tcgagcgtgt
     8581 aagttttgat gagcggcgac gtgtggattc tggaaaccat aagttgctct cgtgcggccc
     8641 ggcggcgcgg gcacgctcca ctcgacctgc tgcgcggctt ggcggcgtcg cgcgtgagga
     8701 gaactcggtt ggcgcgagtg tagcacaggg cgctcccggt tgcgcgggcg ttcggcgcgc
     8761 gcccctctcg ccgcgccgcg ccgaaaacgg tctctttagc cggcatcgca aaccactcac
     8821 ggcgcgtcta aagctttatc atgcgagcga gggaaggtcg tctaatactt tatttcgtca
     8881 tcgccgcggc ggcgctgctg ctggccgcgt gcgggaagaa tcgcgcggga cagggcggcg
     8941 ggggcggcgg gggaagcgtg gccgcaccgg ccgcgggcgt gttgccggtc gccgaggcca
     9001 ccgccccgaa gcggacgctc gcgccgctcc cggcgcgtga gctgacgccg gagctgcgct
     9061 cggcgcgggc gcgggccatg gccgacgctt cgcgcctgcg gggtctcgcg tggcgcggcg
     9121 aggtggggat gacgccgctc tcggcgtggg agttcgggac gcgcgcggcc gagctggccg
     9181 acgcgctcgg cgccgaagac ttgcagacgc tcggccgcct cgccgtcgcc ggcggcatcc
     9241 tcccgccggg cacagacctc gccgcgacgg ccgtcggctt cacggcggcc acggccggcg
     9301 ctttctactc gccgctcgac cgacaggtca tcgtgcgcgc tgaaggcacc ggctcggcgg
     9361 cggcgaagaa gcacgcgctg ctcacgcacg agttcgtcca cgcgcttcag gatcagcact
     9421 tcgacctgct cggcctgctc ctcgccaagc cctataactt cgaccgcgcc gaggccgcct
     9481 tcgcgctcgt cgagggcgac gccacggtcg tccagcgccg cgtcgagcag ggcgacgcgt
     9541 tcgcgcggcg cacgctcgaa gaggtggcgc gagccgagga cgcgcgcttc gactcttacc
     9601 ggcgcgaggt tggctggctc ttccccgcac tcctgaccga gacgtttgtc ttccgctacc
     9661 gcgatggggc gcgcttcgtc gaaacattgc gccgcggccg tccggcaatg ggcgcggacg
     9721 agcttttccg ccgaccgccc gtctcgaccg agcaggtgct ccaccccgag aagtatctgg
     9781 cgggcgaagc gccgcgcgag gtgtctttga acgtcgaggg cctcacgaac gacggctggc
     9841 gagcgagcgg ttcaactccg ctcggggaga ttggcgtgcg cgggctgctg ctggggcgcg
     9901 tcgccgccgc ccgtgcgacg cgtgcggcgg ccggctgggg cggcgacctc gccggcttat
     9961 tcgagcgcga aggccacgcg ccgctcttcg tctggaaaag cgtgtgggac accgccgccg
    10021 acgcgcggga gttctttgcc gcctacggcg ctccgcatcg acgcgccggc gcgagcacgc
    10081 agacgcgcga agaggtctgg cgtgagggcg cggctctgac gctcgtccgc ctcgaaggcg
    10141 acgccgtcct cgttatccgc ggggccgaag atgatgtccg cgcggcggcg cggatgttct
    10201 agcgagcgct tcctgacgcg ccgcgccgcg cgcgccagac gggtcttgct taactaaaaa
    10261 gcagggagta ttcttctcgc agaatccccg gctggactcc gtttcggctt taacacaaga
    10321 gagaggtccg accatgaaag cagccttccg cagcgccgcc aattcagttt tcgcgctcac
    10381 gctcgtcgtc ctctgcgccg gctttacggc cgccgacgcg cagggcaacc gcgacctccg
    10441 cgtcgtctcg gcgcgcgccg gcggggtcaa ttacgccatc ggcgatgtga agctccgccg
    10501 caggggtcag acccgctggc aggcgttcgg cggcgactcc accaccgacc tcgacagcgg
    10561 cgactcactg aagacgggcg cgaccggctt cgccgaagtg ctcctcaacc ccggctcgta
    10621 cctccgtctg ggcgccgact cggagttcgt cctcaccgac tcttcgctcg acgacctcag
    10681 tctccagctc gtccgcggca gcgccgtcat cgaggcgacg ggctacgacc ggatggggct
    10741 gctcatcgcg gtcgatacgc cgcagtcgcg cgtctcgatc attaagagcg gcatctatcg
    10801 tttcaacgtg ctggcgtcgg ggcaaaccga ggtggcggtg cgcaaggggc gcgtgagtgt
    10861 cggcgaggag ccggtgtcgc tcatcgtcaa ggagggcaac gtcgcgcgcg ttgaccgccg
    10921 cgccaccgag gtcgtcaagc tcgacaagaa acagcgcgac gccctcgacg catggagtaa
    10981 aggtcgggcc gacgagctgg ccgaggccaa tcgcaagatc acgcgccgtc aggcgaacac
    11041 actcctcgcg agcctgcgat tcaacgcgaa ctatggttac agcagcggcg ccggcgtgtg
    11101 gtacttcgac acccgccgca actgctacac gttcgtgccc ttcgccgccg gctggcgctc
    11161 gccctacggc ggctggtacg acaacatggt ctggggccac ccgatgccct gcggcggctg
    11221 ctccggtggg cgcaacccgt atgtcttcac ctcgggcggt cagtacggca atccgggcgc
    11281 gacgagcggc ggcggctacc ccggcggcac gactccgggt ggcagcagcg gctatccggg
    11341 cggcggctcc ggcgtcaacc cgccgcccac tcccgtggtg cgcgagcgca cgcccattga
    11401 ctacggcccc gtcaccagaa agatgacgag agaggtagag ccgtaacttc ataaacctga
    11461 aacgtcggcg gcgcgctaca gccgtcgcat gcacgaagac catatcgccc cgccgcgttg
    11521 agcagttcag cgcggcgggg cgatttcgtt tgcggcgatg agggcggagt gtgtgacggc
    11581 cgccacgccc tgaccgggga agaccgtgtc accgaccatg tagagacgcg ggacgctcgt
    11641 ccggtgagtg agggcgcccg cgccgaacac gtcgagcgat tgacccgtgc cgccgaccat
    11701 cccgagacgc cggcgcgtcc gctcgtagca ggtgcgcggc gtcgccgttt cgatcacctc
    11761 ggcgcccgcg ccgagttcgg gcagggcgcg gtgcaggcgt gcccaacact tttcgagcgc
    11821 cgccgcgtcc tgctcctcgt gcgcggcctc gtcgtcgtgg aacgcaaacc actgctcggc
    11881 gtccgtgtgg gtgcagacgg ttgcggcgcg aaagccctcg ggcgcgcgcg cgtcccagcc
    11941 gggcgccgcg ccgagcatca ggagcgactc ctcagggtcg agccgcgcgc cctcctgaaa
    12001 gtccgtgagc gcaaggacgt gcgtcgccgg cagacgcgcc gccgcccgct catccagtgc
    12061 gataaagatg aggtaagcgc cccaaccgcg caggcgtttc atgcgcgcgc gcacgtccgt
    12121 agacgtgtcg gcgaggccga cgagcttgcc gtaggtgtcc cagacggtga ggttgctgac
    12181 gacggcgcgg cgcgcctcga cacgctcgcc gctcagcaga tcgacgccgg cggcgcgccc
    12241 cgaagcgtcg cgggccagcc tcagcacggt cgcgcttagc ctcaccgtcc cgccgctcat
    12301 cttaatcgac tcgacgagag cgtcagccaa cgcttgcgcc ccgccgcgca actcgtacat
    12361 gccgcgcgcg gcgtgcgaga gggcgacggc cgcgtagagg tacgcgcact cgtccgacgg
    12421 gcgttgcgcg aagacgcaga gctgggcgtc gatgaagcgg cggaagcgcg ccgaggtgtc
    12481 gcgcagatgc tcggcggccg tgtggcccat cgagccgaga atgcgcgcgg atagacgcgc
    12541 ctcctgcgcg atgagtttga cgcgctcgat gcgcgtggcg gtgacgagcg ccggcacgcg
    12601 cgcggcggag cgccggagcg cgtcgccgat ggcgcgggcc tcgcggtaga actcaaggct
    12661 cgccgcggcg cactcgggga aggccgcgcg caaagtctcg tcgaactcct cgcgctcgcc
    12721 tccgacgcgc acctccgccc cgtccggcag acgcacgacg taagcgggcg cggcccgccg
    12781 cacctcgggc gcgcccagag gcagctccgc gaacacgcgc tcgtgaatct cgcccggctg
    12841 ccagcaggcg tagagacccg cgccctgctc gaacgcgtag cccatgtgct cgaacgcggc
    12901 ggcacatccg cccgcgcgcg actcgcgctc gaacaggcac acgtcgaggc cgcgtgcggc
    12961 cagcagggcg gccgtcgtca gaccgccgat gccgccgccg acgaccacga catcatgttg
    13021 gctcataagc gggaagcggt gtgcgctaga gcagccacgg cagcagcgcg acgacgaggc
    13081 ccacctgcgt ctccgtgggc tgctgttgcg gcagcgggta gaaatattcg aggacgagcc
    13141 aggcgcactg gatgccgttg aagacgaggt gaatgacgaa acaggggagc aggcgaccgg
    13201 tgcgcgcgcg cacgtaagtg agtgcgaagg cgagaccgcc gaccgtggcg atgacggccg
    13261 ggttgttgta gtactgcgag acgtggacga ccgtgaagag cgtggcgacg gcgacgaccg
    13321 cggcgaacat ccccatggcg cgccggagcg ccgggtagag cacgccacgg tagaccagct
    13381 cctcgacgag cggcgcggtc gccgtggcga ggaaggccgt ggcgaagcgc gcctgcacgg
    13441 acgactccag catctcgtcg aagggcgtct tcacgtcgcg cccgaggaaa tacatcagca
    13501 cgccgcccaa cacgagcagg aggacggcca gcgccgcgct cgtccacagt ccgaagcgcg
    13561 ggctccagcc ccagccgatg gtgcggaaaa aggggcgctt gccgaagttc gtcaccacgg
    13621 cccagacgag cagcagcgtc gcgagatgcg agggaacgac cgacgccacc gagaggagca
    13681 tcatgttcgg gtcgctcatc aacgactcgc ggagttgtcc agaggcgctt atgccctgcg
    13741 tgatgaggac gtaagggatg acgaccagca ggtacgccac gaccatcaac acgatgctcg
    13801 cgagccacac ggagaagccg gcgaggacgc cccacggcgg gttgtcgggg tcgataaggc
    13861 cggcggccgg ggccgccgcg tcgggcgcga agtctgcgaa aggattgtcc gcgacgggtt
    13921 cgaggggggt gctcacgcgg gaagtgtaag agagccggcg tgggcggtca actgggagaa
    13981 taaaagccgt gaatcgtaaa tcgtgaatcg tgtaagacag tgtctccgat tcacgattta
    14041 cgatttacga ttcacggcct ttgtaaaaat gtctttccat cgcggcgcgc acggcggcga
    14101 cgagggcggg caggtcttcg gccgtgaggc cgtcggtctg aaggggcgcg tcgatgtaca
    14161 actcgacggg gccggcgcgc agcaccgcgg agtcgcgcgg catcgcccgg cgggtgccga
    14221 cgatggcgac ggggatgatg ggcaagccgc tttcgatggc gaggcggaag ccgcccttct
    14281 tgagcggctg gagcgagccg tcgcgcgtgc gcgtgccctc ggggaagagg aaggctgaga
    14341 cgccgtcgcg cagagacgcg gacgcgcgac ggaggctctc gacggcctcg gtcgttgagc
    14401 cgcggttgac ggggatgtgg ccggcgcgcg tcatgtgcca gccgaggaag ggaatcagga
    14461 agacctctcg cttggccgcg atgcggagct gcgagggcag atagccgagc atcacgggga
    14521 tgtccatcca actctggtgc gtggacagga agacgtaaga cgcgccgggc cgcacgttgt
    14581 ctgcgccgtg cacacgcacg tcgaggagcg ccgtgcgcgc gatcatccgg ctccacgtcc
    14641 gcgcgcacca gtgctggagc cggccccggc ggtcgaagac cgagagggcg agcgacagcg
    14701 tgcccatgac cacggtgtag agggcgacga gcggccaggc gacgaacgtg tggaggcggt
    14761 taaagagagt cggcacgcgc gccccctccg tgcgcggcac gtccacggcc gcgacgggac
    14821 tgtgaatctc ctcgccgcga gcgtccgatt tcgtgcgcgt ctctgcctgt ctcactttca
    14881 aaggtctggc tcgggttgga acattactgt aggggcagga caagtccgcg gcccgttaca
    14941 cacgaaagga attaacatag atggacagga tgaacaggag aagatttgaa atttgaaatc
    15001 tcaaatcatc ttttcttcat cctgccaatc ctgtccatcc atgttaattg tccgacagcc
    15061 agggccgcga acaagcccta cccctacaaa atctcgcttc taaagagcgg ccgtttccgc
    15121 aacgtcattc gttgaaatgc cgaagtgttg tgcggcgagg atcaggccga tgatcgtctt
    15181 ggcgtcttcg atctcgcgcg aggcaatcat cgacagcgcg cggcggagcg ggagccggac
    15241 gacctcgacg agttcgtcgt cttcgagctg ctgcgtcgcc tccgtgagag cggtcgccag
    15301 gaagacccag agcctctcgg cgcagaagcc cggcgtcggg taaaactccg agagcggctc
    15361 cagccggccc gcgctgaaac cgacctcttc gatcaactcg cgcgccgcac agtcctcggg
    15421 gcgctcgccc gcttcgagct tgccggccgg gagttcgagc aggtagcgac gagccgggtg
    15481 cctgtactgc cggacgagcg cgaccgtgcg gtcgtcgaag acggcgacga ccgacgcgcc
    15541 gccggggtgt tgcacacact cgcgcacgta aatcttctcg ccctcccgca ccgtgtcgcg
    15601 cgtcacgtcg aagatgcgcc cgcggaaaag ctcctcgctg tgcagaacct ccggcgactc
    15661 tttatccact atgtccatct gtcctttgtg tcctttgcgc cttcggcgtc ctctgcgtga
    15721 aatttttcca cgagaaagaa gtttcacgca gaggacgccg aaggcgcaaa ggaaaattta
    15781 ttcgcgctgg cggtcggcag ccgtggccgg ccggccgtcg ctgtagcgga tgccgatgac
    15841 ctcggagtcg agatagaggt gacgctcgcc gccggccgcg tgcgggtcgg cgttgatcga
    15901 gtaggacggt ggacgctccc cggctcgcgg tgtgagccgc agacggaaga cgtagccgtc
    15961 cacggccggg gcgtcgcccg cgaactcctt gttgaggagg atgccggccg cgaggagctg
    16021 gtcgaaggtc gcgtattcgc cgaagttatc gaggtagttc tgctcggcgg cggcgaggtt
    16081 cttgaggtgt tgcgcggccg tggcctcgta ggtcgcggcg gcgcggcggc gcttcgcgtc
    16141 cgtctccgtg ttcaggagca cggccacgag cgccgccagc acggccagcc ctgcgagcgc
    16201 gagaattatc aggatgcgtc cctgtcgagt catgtgtcag caaccgcccc ggtcgaaact
    16261 tcgcgcgccc tcagtctccg agcaggcgcg tcatttcgcc gacgaggtag agcgaccccg
    16321 taacgcagat ggtgccttcg ggtggcgtga cggcgcgcgc aatcgcgagc gcgtcgcgcg
    16381 gcgcgggcgc gagcgagatt gtactcaaat cagtgtcgcg cgggatagcg cgcacgaggt
    16441 cttcgaccgg ggcggagcgc gcattgtcgg cttgcgtgag gacgagacgg cgcgcggcgg
    16501 ggaagagagc gcggccgatc tcctccactt ccttgtcgcg catcgcgccg aaaacgagtg
    16561 tgacgggcgc tcgtgcgaac tcatcgaggt atgcgcgcag ggcacgcgcg ccggccgggt
    16621 tgtgtgcgcc gtcgaagagg acggcgggcg agccttcgcg cagctccaga cgccccggat
    16681 gctcggcgct ctcaagcccc gcgacgacgt tctctggcgt gatctcgaac ccgcgctcgg
    16741 cgaggatttc ggctagccgg acggccgtcg cggcgttcac gagttggtgt cggccgcgca
    16801 ggttgagtcg gacgtgtccg taagtcccac gcccggtggt aaacgagacg cgcaggcgtc
    16861 catcggcgca aatctcttcg acgcgcgcgt ccgatggagc gacgaggcgc ggctcgacgc
    16921 cgcactcgcg gcagcgacgg aggatgacgg ccgcggcggc cggctcctgc ggcgcgatga
    16981 cggcggcggc gacgccgggc cagatgatgg cggccttctc ggcggcgatc tcttcgaggg
    17041 tgcagccgag gtgctcctga tggtcgagcg cgacgggcgt gatggcgacc acaccggcct
    17101 cggcggccgt cgtcgcgtcg agacgcccgc cgaggcccgt ctccagaatc gccacccgga
    17161 cgcccgtctc tttgaagacg agcaaggcgg cggccgtcac ctgctcgaag aatgtcggca
    17221 ccgcgccggt catgcgcccg acctcttcgg cggcggcgcg cacgcgtgtg gccgcgcgcg
    17281 cgaactcctc gcgcgaaact tcaacgccgc ccacgcgcac gcgctcggtg atggagacga
    17341 ggtggggcga ggtgtagagg cccgcacgga tatgtgcggc gcggcagacg gcgtcgagca
    17401 tcgcggccgt cgagcctttg ccgtttgtgc cggcgatctg aatggcgggg taagactttt
    17461 gcgggtcgcc gaggagcgcg agcagacggc ggacgccctc cagcccgagt ttcatcgcca
    17521 gcgtctcgtg gccgagggac agaaggtatc taagcgactc gtcgtaaagc atagttcaaa
    17581 gttcaaagtt caaagttcaa agggccacga cttcaaagtt caaagtttaa ggctcaagtt
    17641 gagaaatgcg cccggcgcgt cggcgcgagc ctgcgggcac acgtccgccc gcatcggttg
    17701 atggtcgcgg ggcgcggggt ctttaacttt gaactttgaa ctttgaactt tgaacttccc
    17761 cctcttcggg ggcgccgttg ttgcccggca gttccaactg gtgcgtctgc ggcgtgaacg
    17821 tcgcctcggg gagcagttcg acggggatgt cgggccgctc catctcgggg ttgtacatga
    17881 attggaggag gcggatgacg gcgtcgcgca tctgccggcg gtcaactacg gcgtcgagca
    17941 tgccgtgttc gacgaggaac tcggagcgtt ggaagtcttt ggggagcttc tgccggatgg
    18001 tctgctcgat gacgcgcggg ccggcgaacc cgatgagcgc cttcggctcg gcgataatca
    18061 cgtcgccgag catggcgaag gaggccgtca ccccgccggt cgtcgggtcg gtcaacaccg
    18121 acacgtaagg caggtgttgc tcggagagga gcgcgagcgc cgccgcgacc ttcgccatct
    18181 gcattaaact taggatgccc tcctgcatgc gcgcgccgcc cgacgcgctg acgatgacca
    18241 cggccgaccg ttcaaagatg gcccgctcga tgagacgcgt gatcttctcg ccgacggccg
    18301 accccatcga gccgccgagg aagctgaagt ccatcgcggc gcagaggacg ggcatgccgc
    18361 ccatctggcc gcgcgcgttg atgacggcct cgggcagccc cgtcgcgcgc cgcgcctgct
    18421 ccagacgctc cttgtagggc ttggtgtcta caaagttcag cgggtcggac gagatcactt
    18481 cgccgtccgt ctcctcgaac ttgccctcgt cgtagagcat ggcgagacgc gcgcgcgcgc
    18541 ccatgcggaa gtgatgagag cagtggtggc agacgtgtag gttctcttcc agctcacgcg
    18601 tgtagagcgc ggcgtcgcac tcggggcact tctggaagac gccctcggtc ttaacgcgcc
    18661 gctcgtcggc cggcaccggc tcgatgcgct gattcttttt acgaaaccac ttcgacattg
    18721 gctcacacta gcggggacgc tctacggtca cgaagggtat cacaacgcga cggccgggct
    18781 gaagtgccgc ccggccgtcg ttcgctttcc cagttgcccc ggcgcggccg actacttgcc
    18841 gacgcccgac atttcggcca ccggctcgcc gccgcccggc agcgggggcg gcgcgccgtt
    18901 cgagcggcgc tcgacggcct cgatgaggtg ggagacgatc atctcctcgg tctcgtccca
    18961 atcgccgccg agggtgcagc cggcggcgtt acggtggccg ccgccgccga agcgctcggc
    19021 gatgcgcgcg acgttcacgt cgcccttcga ccgaaggctc acgcgataga ggcccggctg
    19081 cgtctccttc aggaaggcga cggcctcgac ctcgccgcac gagagcgggt aattgacgaa
    19141 cccgtcggcg tcctcttccg acgccccggc ctgcgtttgc atgtcgagcg tctgccgcag
    19201 ccacgccacg cgccccgtgg ggtcgcggtt gacggtcgaa agaacgaggc tcgtcaggcg
    19261 tatcttgccc cacgcgtagc tgccgaaaac ggcctgcgag accttcgccg gcttgacccc
    19321 ggcgcgcacc agctcggagg ccaccttgaa ggtgcgctcc gtggtgttcg cgtagtggaa
    19381 gctgccggtg tcggtgatga gcgccgtgta gacgcactcg gcgatctcgc gggtgacgcg
    19441 cacgccgatg gccttgcaga ggttgtagat catctcgccg acggccgagg ccgtcgagtc
    19501 gatccagttg atcgtgccga agagcgaggt ggtcgagtgg tggtcgatgt tgacgacgag
    19561 ctgttcttcg aggcgttcga ggccgggccg cgtgatgtcc gaacactcga tgacgaagac
    19621 ggcgtcgtag ggcttatcga ccgaaggggt gacgcggatg agttccgccc ccggcagctt
    19681 gtggtaggcg tggggggcgg cgtcgcgcat gatgacttcc gcctctttgt cgagggagcg
    19741 cagaagccag tagaggccga gcgacgaccc gagagcgtcg ccgtccggcc tcacgtgcga
    19801 agtgatcgcg aagcgccgct tcgcctcgat cagctcaact acttgactaa gcatttcaaa
    19861 gaagtgatga gtgatgagtg ataagtgata agagaagaca ggcgcttttc ttatcactta
    19921 tcacttatca cttatcactt attttctcct ttttcttctg tttccgccgg gccgtccccg
    19981 gcggcggcga cgctctcgct gctgctgccg ctgtcctgtt tcacctgcgc gagcagctct
    20041 tcgacgcggg ccgccttctc ctcgaccgtg tcgcggacga agtgcagctc gggcgtaaat
    20101 ttcaggttga gcgccagccc caactggcgg cgcacgtagg gcgcggcctt ggtgagcgcc
    20161 ttgagcgcca gcgtcttttc gtcgtcggtg ccctcgacga ggacgaaaac gcgcgcgtcg
    20221 cgcaggttct ccgtaacgcg cacgtccgtc accgtcgccg tctcgacccg cgggtcttcc
    20281 agctcgtagc cgacgatctg cgacagctcc tcgcgaatca gttctgcaag ctgctgtggg
    20341 cgacgcataa aaacagaggt tagaggttag aggtcagagg ttagtgaaga cagtttttaa
    20401 ctgacctcta acctctaacc tctgacctct tacagttcag tggcggccac cgactcgatg
    20461 acgtaagcct cgatctggtc gttgaccttg atgtcgttga agttttcgag tccgatgccg
    20521 cactcgaagc cttcgcggac ttcggccgcg tcgtccttga agcggcgcag ggaagctatc
    20581 tggccctccc aggcgacgac gccgtcgcgg atgaggcggg cgcgggcctt gcgggagatg
    20641 acgccgctgg tgacgcggca gccggccacc gcgccgatct tcggtacgcg gaagacctcc
    20701 tgcaccgagg cgttgccgat gatgcgctcg gtctcgatgg cgtcgagcat gccgagcatg
    20761 gcggcgcgta tctcctcctc gaccttgtag atgatcgagt ggagacgcac gtcaacgtgc
    20821 tcgtgcttgc caagctcggc ggcccgcacc tcgggccgca cgttgaagcc gatgatgacg
    20881 accgcgctcg aagaagcgct cgcctgcgtg gccgaggcca ggagcacgtc cgactcggtg
    20941 atggccccga cgcctgagcg aatgaccttg accttgacct tctcggtcga gagcttctgg
    21001 agcgtgctct tcaaaacttc gaccgagcct tgcacgtcgg ccttgacgac gatcaaaagc
    21061 tccttcgtct cctgctgccc cagcgcctcg atgccgcgct tggtggtctg gacgagggcg
    21121 gcctgacgcg aaatcatctg gcggtgctgc gaaatcgact gcgccttcgt gatgtcggcg
    21181 accacctgga aagtgtcgcc ggcctgcggc acgccctgca tgccgagcac ctcgacgggt
    21241 gtggccgggc cgacctcggt gaccagttcg ccgcggtcgt tgaacatggc acgcaccttg
    21301 ccgaaaatct gtccgacgat gaacgggtcg tgaaccttga gcgtgccctg ctggacgagg
    21361 acggtggcga cggcgccgcg cccgcggtcg agcttggctt cgagcaccac gcccgaggcg
    21421 agccgcgtgg ggctggcctt gaggttgagg atgtcggccg tgagcaggat ggtctccagc
    21481 agcgtctcca gattctcgcg cttcttggcg gagacggcga ccatctcggt ctctccgccc
    21541 caatcgacgg gctggaggcc gaggccggcg agtccctgct tgacgcggtc ggggttggcg
    21601 tccggcttgt cgatcttgtt gatggcgacg acgatgggca cgccggccgc cttcgcgtgc
    21661 tcgacggcct cgatggtctg cggcatgacg ccgtcgtcgg ccgcgacgac gagcacgacc
    21721 acgtccgtgg ccttcgcgcc gcgggcgcgc atgagggtga aggcttcgtg gccgggcgtg
    21781 tcgaggaaga cgacgcggcg cagttggagc ggatcgtcgg cgctggccac ctgaacgctg
    21841 taagcgccga tgtgctgcgt gatgccgccg gcctcgccct cggcgacttt ggcggtgcgg
    21901 atggcgtcga gcagggaagt cttgccgtga tcgacgtggc ccatgacggt gacgacgggc
    21961 gcgcgcgcct cctcgacgta gtcgtcgatg ttctcaccgc cgaggatgtc ttcaagctcc
    22021 tgccccgtga ccatgtcctc gtagggcatg aaggagacct cgaagccgaa gcgcttgccg
    22081 agatcgacgg ccacctcgtc gtcgagcggc tggttgatgg tggcgaacac cccgcgctgc
    22141 atcagcaggg tgacgacctc cttcggcttg atgccgagct tctcggcgaa ctccttgacc
    22201 gtggagcctt cgacgaggct cacctgcttg gtctcgccgg cgagcgcgag gttctgcgcg
    22261 aaacgctgct cgggcgtgag cgccttcggc ggcaggacga aatctttctc atagcggccg
    22321 ccgcgttcac gcgggccgcc cgagaggcgg cccgtgccgc ggcgcgagcc ggggcggccg
    22381 ccggggcggc gcgcgtcggc cggtgggatg taggtcgtct gcggcgtggc gctctcgccg
    22441 ggcgtgccgc gcagcgcctc gtcgcgtccg ccgcgcccgt tgcgtggccg ctcgtcgcga
    22501 ccgccgccgc cactacgggc gcgcgcggcg gccggcggcg tcaccacggc tggcgcaaca
    22561 tcaggcgcgc gctccccgtg gcggatgccg gcgttgagcg cggcggtcgt gggccgcagt
    22621 gctctgacct gtcgcgccgg cgcgggcgtg gccgaagggc cactcggcac ggcggcggcg
    22681 ggcacggtct ccatctctgt ctcttctgtg gcgtaaacgg ccgccggggc cggcgtctca
    22741 aactcctccg tctcaactgc cgccgcctct ggctccacgt aggaaacttc ctgcgcctcc
    22801 gccggggcct cggcgacagc cgcctcctcg ggcctctcgg cgcgcgtcgc cgcgggtctc
    22861 ttgacgagac gcgccgggac gagtctggca ccgccgccgc cgccggccgc gggcggctcg
    22921 gacacttcgg gcgcgtgggc ctccaacgtg ggcggcgcgg gcgtcgattc cggcgctggc
    22981 tccatcgtca cgggggcatg ctgcggcgag tgctcgtcgt ccgccgccgg cgtctcggtc
    23041 tccacgggcc gggcggcctt cttgacgacg cggatgctgc gcggggcgtg tacttccttc
    23101 ttcgggaaat acttgttgcg aatcttctcg gccagctctt tggagatcgt gttcgagggc
    23161 acggacacgt ccacgccctc gcggcgcact tcttcgatga ggcgcttcga ctccatcttc
    23221 agctctttgg cgaggtcgta gatacgaatc ttggacaggg cggtggtcat gcactccttc
    23281 ttcgacgctt aaaataaaac ggcgtcgcac gcttcaaagg ttgaggcccc tcgtcgaggt
    23341 ctcgaaaccc cttcggccgt gcgacgcccc ctctcgcttg ggaaggcgtg cctcgggccg
    23401 tgaggcttca gggcgggcag gcgcacgcgc gcccgtcgcc cggctgctga tggttacgcg
    23461 ttgccggaag catcgtcagg cttctcactc ggggcaacgc cttcgtccgg ctccgacgcc
    23521 tcgacccgtt cggagtcgga aggcacgtcg gactgggcga cttccggggc ggaggcttct
    23581 ccacccaggt cggcggcggg cgaaagcggc gagtcgtctt cctcttccgc ttccgtccgc
    23641 tccagctcca tggcggcgcc gctcgtctca agcacggccg cttcggagga tgtgatgtca
    23701 ggcgcggggg tgatcgtgtc ggcgcgcaga tcgcgtcccc ccccctgcaa gagaagctcg
    23761 tcggccgaca tcgggtcgga ctcggagagc gcgaccggct cgcccgactc tcccgtcagg
    23821 tgatcgggat cagccgtgaa cggcgtccca cccgcgaccg ccgcgtcgta cccttcggcg
    23881 atcatcgctt ccgacggctc gacctcgcgc gcgggcgcat cggggtcgaa ctcgaacgac
    23941 gcggcgtgct gctcgacagt gccgcgcgtc tcttccgcgc cgccgtcgct caacgtctcg
    24001 ccggcgtctt caacagcgcc cgtctcgtcg tcgccgccct cggaaacttc ggtagaagcc
    24061 tcggcgtcgg gcgacgcttc ggcggccgtc tccgaccccg cgtccgtgtc agtttcggag
    24121 ccgtcgccgc ccgtctccac ggccgcggtc tcgtcggttg cggcagcgtc cccggcttct
    24181 gcggccgcgg cctcttcgtc gctctgctgg cccgcctcgc gcgcggccga gacgatggac
    24241 tgcgccgaag agatgatgcg ctgggcgtcg tcgatgctga ggtcgaggta ttcggtcagg
    24301 tcgtcgatgg acgtggtggc cagcgtctcg atgtcctcga tgccgcgaag ggcgagcgcg
    24361 tcgatcatcg ccgggttgat gccgtcgagc cgctccagcg gcacggcctc gcccgaggcg
    24421 atcatcgcgc ccatctgcgc ggcgacctcg cgcttgatct cttcctcact gcggatgtcg
    24481 atgttccaac cgacgagctt ggtggcgagg cgcacgttct gcccgcgctt gccgatggcc
    24541 aaagatagct ggtcttcgtt gacgatgacc tccatctgcc ggcgctcgat gtcggtgatg
    24601 cggacctgat tgaccttggc gggggagagc gagttggcgg cgaacaccga tggctcgtcc
    24661 gaccactcga tgatgtcgat cttctcgccg cgcagctccc ggatgatggc ctgcacgcgc
    24721 gaacccttca tgccgacgca ggcgcccacc gggtcaacgt cgcgctccgt ggaggcgacg
    24781 gcgatcttgg cgcgctcgcc cggctcgcgc acggccgact tgatgaccac cgtgctgtcg
    24841 taaatctcgg gaacctccat ctcgaagagg cgcttgagga gttccggcga ggtgcgtgag
    24901 acgatgacct gcgggccttt ggattccttc gacacgtcgt cgatgacgac gcggatgcgc
    24961 tcgccctgat tccactgctc gccgcgcgac tgttccttgc ggccgaggag tgcctcgacg
    25021 ttgccgagat cgacgatgat gtcgccccgc tcgaagcgct tgatgtagcc gttcacaagg
    25081 tcgccgacct tgtcgatgta ctgctcgtag acgttctgcc ggacggcgtc gcgcaccttc
    25141 tggaagagga tctgcttggc ggtctgtgcg gcgatgcgtc ccatttcttc catcggcatc
    25201 ggcagttgca gctcgtcgcc gacctcgacc tcgtcgcccg ccagctcctt cgcctcgtcg
    25261 agcgacagct cgcgggccgg gttctcgacc tcctcgacga caaccttggc ggcgaagatt
    25321 tccaggccgg actcagtcgt ccactcgacc tgaatctcct ccttgaattg tttgcgcgcc
    25381 gcggccttca cggcctcctt catcgcgtca ataacgatgt cgcggtcgat gccgtgctct
    25441 ttgcagaggg cgtcaatgct ttgcggaatc gatgcactca taagattcag aggttagagg
    25501 ttagaggtta gaggttagtg aaatcagttt ttactgacct ctaacctctg gtctctgacc
    25561 tcttgctttc aactttctgc cgactcttcc gcttgcgcgg agcgcaactc ccgctcgcgc
    25621 tgctcggcgc ggcggaactc ttcctccacg tcgatttcga ggttcgcctt gacaatcgac
    25681 gcgacgctca ccagaacgcg gcccgtcgtc acgtcttcaa aaaccacttt gtcgccttcg
    25741 acgccgacga ttcggccgcg gaagttgcgc tggccgtcca cggcctcgcg cgacttcatc
    25801 cgggccaacc ggccggcgaa tcgctcgtag tcctcgcgcc ggtaaaggcc gcgctcaaga
    25861 cccggcgagg agacttcgag gttgtaaggc tcgtggatga agtcctccac gtcgaggacg
    25921 gtgctcatgt ggtggctcac ctcggagcag tcctcgtggg tgacgccgtc gggcctgtcg
    25981 atgaagacgc gcagcgtgcg caggcgcccg gtgcgggcca tctccacgtg cacgagttcg
    26041 aggccgcgct cttcggccgc gcgcgtggcg atctcgcgca cccgcccctc gactgaatcc
    26101 ccgtccgtca ccgcccggcc tccccaaaaa aatgcccttc gcgcaaaata aaaagtgggc
    26161 gcgaacccac taggacaacg ccccggcgct ccgagcaaga ggcgaattat acggaaacac
    26221 cgggcgcgtg gcaacccgcc agtaaagtga gcagtgagca gtaagcggtg agcagtaaaa
    26281 agccccttca ccaccctccg gaacacgggc cgcccgctcg cggtttaccg cccgccgctc
    26341 actttactgc ttactgctca atgcttacgg ctcactgaag tctggccttg acgatctcgc
    26401 tcactatccg cccgtcggcc gccccgccca gtctcccttg tacggccttc atcaccgcgc
    26461 ccatctcctt catcgacgtg gcgcccgtct cactgatgac ggccgcgacc gccccctcaa
    26521 tctcctcgcg ggaggccgcg cgcggcaggt actcgtcgat gacggcgatt tcggcgagtt
    26581 ccttctcggc cagctcgttg cgccccgcct tctcgtactg ctcgaccgag tcgcgccgct
    26641 gcttgacgag cgacagcagc gacttcgtcg tctcctcgtc cgtgagttcg ccgcccttct
    26701 cgatctcgcg gttctggagg gccgccttga ccatccgcag cgtcgaggtg cgcgccgcgt
    26761 cgcgcgcctt catcgcggcc gtcatgtcgc tcacgatccg ttgcttcaga ctcatgctga
    26821 cagaataaac aaggatgggc gggagaggga aggggcgggg acggcccagt gagcagtaag
    26881 cagtaaggag taggaattcg ggagccgccg gaggcggcgg aagattgtag ccacgggcgc
    26941 cagcccgtgg taacggcgga ataaaatatg agaagccccg gaggggcgaa agaatcccga
    27001 cgttgggggt gaggcccgtc acggttcttt cgcccctccg gggcttcaat gtttgttgtg
    27061 gcgtctgacc cacggctcgc gccgtgggct acaatcttcc gccgctccgc ggctcccaaa
    27121 ttcctaccgc ttactgctta ctgctcactg ctcactgctt actcctgact gctgctttgc
    27181 ggccggctgc gcccggccgc ctcgcgcagc tccctgagcg tggtcgaggg gccgtggacg
    27241 gaggcgatgc gctccacgtc gaggccgagg cgcgtgatgg tttcgaggaa gtgcgtggtg
    27301 gcctcgatga tggtcgtggg catgtacttg cccgagttgg gcaggctcac gaggtcgccc
    27361 tgaaagacca gtcgttcctt cggcaggtag gcgacgagca tctcgcgcgc gtgcgggttc
    27421 ggccctacgt cgatgacctc gacgctctgt ttgccgtccg tgaagacgcg cttgccgttt
    27481 tgcacgaact cgaaggcggg cttgcggagc gcgcgtgact ggtcgtcggg cgtaacggtg
    27541 aaggtggacg cggccatccg ctcgaagaac ttctggttgg cagcggtcgt gacgatggtt
    27601 gcgcccgcgg cgacgtaggg gcgcaggccg cccgagtggt cgtcgtggaa gtgtgtgacg
    27661 acgacgtagc ggatgggctt ggcgggaaac atctccttca gcttcgtgat ggtagctcgg
    27721 ctgccgcgcc cgcccgcgta aggctcgacg acgaggaggt ggtctgagaa ctcgacgacg
    27781 agcgagttgg cgccgctctc gaagaggtgg acgccgtcgg cgagcttcgt ctcgcgcgtg
    27841 ggcgcaggac caggcgtcgg cagctcctcg tgcccttcgg gcttggcgaa ggcgctctcg
    27901 gcgggcttgg tgttgaactg cacgtcgtcg taagtgactt cttcgataag ttcgccggcg
    27961 cggcgcgtga cgcggcccgt ggggactttc acgcggccga ccgtgcggta gccggggaag
    28021 gcccactcgt gtgtggtgtc gccggcgagg ttgtcgctca ccacgatctc ctgcttcgag
    28081 agcaggttgg tcgaggcgtc gaagtagagc gtccactgga cgccgttcgg cgtggcggcg
//
LOCUS       DASVES010000480         9539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_484315, whole genome shotgun sequence.
ACCESSION   DASVES010000480 DASVES010000000
VERSION     DASVES010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484315"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /gene="tsf"
                     /locus_tag="VF240_20130"
     CDS             complement(<1..641)
                     /gene="tsf"
                     /locus_tag="VF240_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7177578.1"
                     /translation="MAEITAAAVMALRQKTGAGMMECKGALTEAGGNEEQAIDLLRKR
                     GLASAKKKEGRIAAEGAVGSYIHMGGKVGVLVEVNCETDFVARTEAFQSLVKDIAMHI
                     AAAEPRYVARAEVPADLVEKEREIARGQAKNDPKNASKPEQVIDKIVEGRLNKFYEEA
                     VLLDQPFVKDPAKTIQDLLTEKIASIKENITVRRFARYKMGEGLEKRADDFGG"
     gene            complement(869..1843)
                     /gene="rpsB"
                     /locus_tag="VF240_20135"
     CDS             complement(869..1843)
                     /gene="rpsB"
                     /locus_tag="VF240_20135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7177579.1"
                     /translation="MAVTVTMKELLEAGVHFGHQVRRWNPKMKEFIFGERNGINIIDL
                     QKTQKMFRDAIAFVTALAGEDRGRTVLFVGTKRQAQDAIREESVRAGQYYINNRWLGG
                     LLTNFQTVQKSIKRLRDLEAMQTDGRYEMITKKERIKFDREREILDKNLAGIKNMTRV
                     PDALFIIDVRKEEIAVAEANRLGIPVIAVVDTNCSPEGIDYVIPGNDDALRAIRLFAS
                     RMADAVIEGAQMRAKGNEEEEEPAPAESQNAADMPAAAPSAETQTSVPGNIGFGKTGQ
                     QPDSVGDHEQAGTGAPVRGATGGDAAEGTQPARSPEQDKGAETVGVAS"
     gene            complement(2026..2418)
                     /gene="rpsI"
                     /locus_tag="VF240_20140"
     CDS             complement(2026..2418)
                     /gene="rpsI"
                     /locus_tag="VF240_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7177580.1"
                     /translation="MADIQYYGTGRRKTSTARVYLRPGTGEFRINRKTFEQYFPNQTL
                     RMIISQPLKLTDTVDKFDVLVNVAGGGPSGPAGAVRHGITRALLEYNSELRRTLKQAG
                     LITRDPRIKERKKYGQKGARKRFQFSKR"
     gene            complement(2446..2910)
                     /gene="rplM"
                     /locus_tag="VF240_20145"
     CDS             complement(2446..2910)
                     /gene="rplM"
                     /locus_tag="VF240_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7177581.1"
                     /translation="MSTYFPSGQGLADNRKWYIVDAAGQTVGRLATEVARVLMGKNKP
                     QWTPFIDMGDHVIVVNARKAVFKGAKATQKVYHRHTLYPGGLRSVTVEEQFKKHPERV
                     IEIAVRGMLPKTKLGKAMGKKLKVYGDAEHRHAAQQPEALPLSARADAGAKK"
     gene            complement(3072..4280)
                     /locus_tag="VF240_20150"
     CDS             complement(3072..4280)
                     /locus_tag="VF240_20150"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7177582.1"
                     /translation="MTSNFSRGLTALALSASLLAGSASALAAGQDKTATDAKTSDQKD
                     AKAKKDSQKSAPAVVQTSANKTLSENEDPRLIGKRKINKGTVFSSGGSLEKEVALGRQ
                     LSAEVDRQAKFVDDPTVTEYVNRVGQNLVLHSDAKIPFTIKVIDSDEVNAFALPGGFF
                     YVNKGLLLAADNEAELAGVMAHEIAHVASRHAMENMRKGQWLEGLAMVGSIFLGGIPG
                     MIYQSTAGLGLAAAFFKFTRGAEEEADRLGVQYLWAAGYDPSAMSTMFEKLNAQNKKK
                     PGFFARTFTTHPQPPDRVEATRVLVARFPDREEYVISTSEFQRVKNRLLRLSNSRVSS
                     TGDLAAGDDSGGAPGRPTLKRRQPTAEETGPATDATKEEKNEAPRLKRRTGDPATTTT
                     PAPTQPGNNP"
     gene            4785..8411
                     /locus_tag="VF240_20155"
     CDS             4785..8411
                     /locus_tag="VF240_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177583.1"
                     /translation="MKRTQDKIKDIVEPQDFDEVRNVVSDPARSLAAYCFTDATSDLL
                     ARWLDALADLPRGRGAARSLAGVRGVGKSHALAVFGALAAQERLRLDVSDAHVATSAR
                     RLSGRRYMVVRVERGTRDTLAEEMAAAFGRAFGGSEAQWGTDPAGMLAVAGSRAHDST
                     LVLIIDTGFDRPSRVRRDDGTHLGEIARTARGANAFVALALDDDISGADGPNVAIAGT
                     YHIDFLDHEHLFRIADTYILHKNERGRAALHDIYLHLRASVPGFNWSEPRFAALYPVH
                     PLVADVAAAVRLYAPTFAFLPFAAEAARRATSRPALSLVLLDEVFDRAERDLRKSPEL
                     KEAFEAYDDLATRVVAQFPVMQRLEAKLVLKTLFILSLDGGGATPQGLCAALLVSQES
                     APAGGAARVSEMLDRFVEAAPEALRVGDEAAAGDGPRYRLQIGALDKFQTALERSVER
                     LPDGSDSLALTQLRAAAHARFEDWPFADGHDEQTATANFNLAWRGSERRARMVWVDAA
                     ASPPAAAPDFDLEVFIYAPGAEATPAPPAATDEDAGAAPLSVVWRPGEVTAEELTTLR
                     RLLALRTDGTLAADFTETAVAAETVLAAQAQRVWARIYLDAGTLEVGGERKSPDDRAR
                     GSRTLSAALSTTLAPHFAELYPQHPQFAETLRDADVERLSAGLFAGAGASDAEVERLA
                     RVFALPLGLVERRGNEHILAPGDDGADDPAWVKEVLRLVAHTDAASAPLTSASPATVP
                     LESIRGALRRPPYGLQREAQNLLLAALAAQRRIELFDASGERALRPGRPLNWDEVASV
                     APAAQQGRDPEQSAAWARLLTGRPDLRALSDREARGAASMALSEWLERWRALKLGERF
                     DALPDALLTTRVWRMNAEACRRFGAAAEAVRLVLASELSLDEGLERVADAFDNSADTL
                     AEAAHDLDALTQFVENCVARRRAAAYLLTAGQTGVDEIEAARRELLSIARERACLFDA
                     ERNERFNRLWPEFQTLYAEHYAAAHDAAGAHTRRALDTLLAGDRWREFEMLSRLPFVT
                     RQYWEAARELLRRAGAPRCDLPVRQLLAARTACACGFKLSGADAASRLPREFEEVLES
                     GLAGYRRTLALLAAPLRRALEALAGREEERRETATRARILAAAFGAGTPPRRFSRADA
                     QLIERAAAEMGAPPSVRASVPTDGGQLLTRDELSARLQQWLDDLPANAALVELVAGGS
                     ETHA"
     gene            8404..9477
                     /locus_tag="VF240_20160"
     CDS             8404..9477
                     /locus_tag="VF240_20160"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEX7177584.1"
                     /translation="MPDRKAAKGKAGRGKGRRGTGDRGRGRKRTVLPSPLPLPLCPFP
                     RLSIAVVGAGRLGTALARSLAAAGCEIDAVVARRIARARRAAALIGTKAPALSAAQLE
                     RLPKVDLLLLTTPDDALPEVVARLAALGKDDETPARPKVGARSRGAGAVALHASGALG
                     SEVLAPLRERGYAVGSLHPLVSVSDARAGSEIFRGAYYCVEGDPAAVRLARRLVRALG
                     GQSFTVATDRKALYHAAAVMTSGHTVALFDTAARLLALCGLTPARAREVLLPLLGSTF
                     ENLSALPPPRALTGTYARADADTVRKHLSALADAGDHNALTVYTLLARHSLLLAEANG
                     ADPTSLKEITRLLERYSLEWKRS"
BASE COUNT         1391 a         3367 c         3140 g         1641 t
ORIGIN      
        1 tcgccgccga agtcgtcggc gcgcttctcc agcccctcgc ccatcttgta gcgggcgaag
       61 cggcggacgg tgatgttctc cttgatggag gcgatcttct ccgtcagcaa atcctggatg
      121 gtcttggccg ggtctttgac gaacggctgg tcgagcagca ccgcctcctc gtagaacttg
      181 ttgagccggc cctcgacgat cttgtcgatc acctgctcgg gcttgctggc gttcttcggg
      241 tcgtttttgg cctgaccgcg cgcgatctcg cgctccttct cgacgaggtc ggccgggact
      301 tccgcgcgcg cgacgtagcg cggctcggcc gcggcgatgt gcatcgcgat gtccttgacg
      361 agcgactgga aagcctcggt gcgcgcgacg aagtcggtct cgcagttgac ctcgacgagg
      421 acgccgacct tgccgcccat gtggatgtag gagccgactg cgccctcggc cgcgatacgc
      481 ccttccttct tcttcgccga ggcgaggccc cgcttgcgta gcaggtcgat ggcctgctcc
      541 tcgttgccgc ccgcctccgt caatgcgccc ttgcactcca tcatccccgc gcccgtcttc
      601 tggcgcaggg ccatgaccgc ggctgctgta atctccgcca tgtgtttcaa ctctccttga
      661 tagcttaatg atgaaaagga agtcaggaga caaaagtcag gagtcagaat aaaatccgtg
      721 cccggccgcc tcgtatattc tgactcctga cttctgtctc ctgactcctg agtcgttaaa
      781 aagcgcagcc ccggcgggtt catccgcgcc ggcaggctgc gctggccgaa gagtaatctt
      841 accgacctac ggtcttcaaa tctcgactct agctcgccac gccgaccgtc tccgcgccct
      901 tgtcctgctc gggcgagcgg gcgggttgcg tgccctcggc cgcgtcgccg ccggtcgccc
      961 cgcgcacggg cgcgcccgtc ccggcctgct cgtgatcgcc gacgctgtcg ggctgctggc
     1021 ccgtcttgcc gaagccgatg ttgccgggaa cgctcgtctg cgtctcggcc gaaggggcgg
     1081 cggccggcat gtccgccgcg ttctgcgact cggccggggc cggctcctct tcttcctcgt
     1141 tgcccttggc gcgcatctgc gcgccctcga taacggcgtc ggccatgcgc gaagcgaaga
     1201 ggcgaatggc gcgcagggcg tcgtcgttgc cggggatcac gtagtcgatg ccctcgggcg
     1261 agcagttcgt gtccacgacg gcgatgacgg gtatccctaa gcgattggcc tcggcgacgg
     1321 cgatctcctc cttacgcacg tcgatgatga agagcgcgtc gggcacccgc gtcatgttct
     1381 tgatgcccgc gaggttcttg tcgagaatct cgcgctcgcg gtcaaacttg atgcgctcct
     1441 tcttggtgat catctcgtag cggccgtcgg tctgcatcgc ttcgaggtcg cgcagccgct
     1501 tgatcgactt ctggacggtc tggaagttgg tgagcaaacc gccgagccag cggttgttga
     1561 tgtagtactg cccggcgcgc acggactctt cgcggatggc gtcctgggcc tgccgcttgg
     1621 tgccgacgaa gaggacggtg cgcccgcggt cttcgccggc cagcgccgtg acgaacgcga
     1681 tggcgtcgcg gaacatcttc tgcgtcttct ggaggtcgat gatgttgatg ccgttgcgct
     1741 cgccaaagat gaactccttc atcttcgggt tccagcgcct gacctggtgg ccgaagtgga
     1801 cgccggcctc caaaagctcc ttcatggtga cggttactgc caaactaaag cctcctctac
     1861 agttgattgg tttagtgtac tcgccgcgcc ccacgcgccc cctccgcggg gcaaccaacc
     1921 ggggcgcggc aatggatttt gcggtgatga gtgataagtg atgagtgata agagtaagaa
     1981 aagtttttct tatcactcat cacttattac ttatcaccgt cttctctatc tcttcgagaa
     2041 ctggaagcgc ttgcgcgcgc ccttctggcc gtacttcttg cgctccttga tgcgcgggtc
     2101 gcgcgtgatc agcccggctt gcttgagcgt gcgccgcagc tcgctgttgt attcgagcag
     2161 cgcccgcgtg atgccgtggc ggacggcgcc ggccggcccc gaggggcctc cgccggcgac
     2221 gttcaccaaa acatcgaact tatcgaccgt gtcggtgagc ttcagcggct ggctgatgat
     2281 catgcgcagg gtctggttgg ggaagtattg ctcgaaggtc ttgcgattga tgcggaattc
     2341 gccggtgccc ggcctcagat agacgcgcgc cgtcgaagtc ttgcgccgtc cggttccgta
     2401 atactgaata tctgccacag ctcgtccttc ctcgaaaatc ctggtttact tcttggcccc
     2461 ggcgtcggcg cgcgcgctca aggggagcgc ctcgggctgc tgggccgcgt gccggtgctc
     2521 ggcgtcgcca tagaccttca gcttcttgcc catcgccttg ccaagcttcg tcttcgggag
     2581 catcccgcgc acagcgatct cgatgacgcg ctcggggtgc ttcttgaact gctcctcgac
     2641 ggtgacggag cgcaggccgc ccggatagag cgtgtgccgg tggtagacct tctgcgtcgc
     2701 cttggcgccc ttgaagacgg ccttgcgcgc attgacgaca atgacgtggt cgcccatgtc
     2761 gatgaagggc gtccactgcg gcttgttctt gcccatgagc acgcgcgcca cctcggtcgc
     2821 gaggcggccc accgtctgcc cggccgcgtc cacgatgtac cacttgcgat tatcagcgag
     2881 accctgcccg ctgggaaagt aggttgacat aggttaagtc tctctaaaat aagcgtctaa
     2941 aacgcgaacg ggtagaatac gaaacggccg aaaagatgtc aagccgatgg ggtttgtgcg
     3001 ccgcttcggc aaaaaggctc tcaggccgct ttgcctgaga gcctcgttgt tcgatgtagc
     3061 cgttaaagcg gttacgggtt gttgcccggc tgagtcgggg ccggggtcgt ggtcgttgcg
     3121 gggtcgccgg tgcgacgctt gaggcggggc gcctcgttct tctcctcctt ggtcgcgtca
     3181 gtcgccggcc ccgtctcttc ggcggtgggc tggcggcgct tgagcgtcgg gcggcccggc
     3241 gcgccgccgc tgtcgtctcc cgcggcgagg tcgccggtgg acgagacacg cgagttcgac
     3301 agacgcagca ggcgattctt gacgcgctgg aactcggagg tcgagatgac gtactcctcg
     3361 cggtcgggga agcgggcgac gagcacccgc gtggcctcga cgcggtcggg cggctgcggg
     3421 tgcgtcgtga aggtgcgcgc gaagaagccc ggcttcttct tgttctgcgc gttgagcttt
     3481 tcgaacatcg tggacatcgc gctcgggtcg tagccggcgg cccacaggta ctgcacgccg
     3541 aggcggtcag cctcctcttc agcgccgcgc gtgaacttga agaaggccgc ggcgaggccc
     3601 aagcccgcag tgctctggta gatcatgccg gggatgccgc cgaggaagat cgatccgacc
     3661 atcgcgagtc cttcgagcca ctggcctttt cgcatgttct ccatcgcgtg acgcgaggcg
     3721 acgtgcgcga tctcgtgcgc catcacaccg gcgagttcgg cctcgttgtc ggcggcgagg
     3781 agcagcccct tgttgacgta gaagaagccg cccggaagtg cgaaggcgtt gacctcgtcg
     3841 gagtcgatga ccttgatggt gaagggaatc ttggcgtcgg agtggagcac gaggttctgg
     3901 ccgacgcggt tgacgtactc cgtgacggtc gggtcgtcaa cgaacttggc ctggcggtca
     3961 acctcggcgg agagctggcg gccgagcgcg acctcttttt cgagcgagcc gcccgagctg
     4021 aagaccgtgc ccttgttgat cttgcgcttg ccgatgagac gcgggtcttc gttctccgag
     4081 agcgtcttgt tggcgctcgt ctggacgacg gccggcgcgg acttctgcga gtcctttttg
     4141 gctttcgcgt ccttctgatc cgacgtcttt gcgtcagtgg ccgtcttgtc ctgcccggcg
     4201 gcgagcgcgg acgcggagcc ggccagaagg ctcgccgaga gggcgagggc cgtgagaccg
     4261 cgggagaaat tactcgtcat gtggaagtgc ctccaaaaaa atcgtaacaa gcgacagatg
     4321 ccgtaaaggt tcgtcgggcc gccctccggc aacggaaggc gctcatctcg ggcggttacg
     4381 gccgatcaac cgaaccccgc cgcaccccga cggcgcgacg cgggccgaca acacgacaga
     4441 tttcaaagga gcgcatggcg ccgggcgtag ttcaaaagag atttcggacg ggcctcggga
     4501 aacgcgcaag tctttgatgc gccggcccgc tccgcgggtt gcgccggggc gcctcagctt
     4561 tcgcggcgcg acgcggccgc cccgaaactc ccccgtcgcg ggagcggtgc gaaacgtctg
     4621 ctctgctacg tttgttccgc gacgattata gggcagaacg cgcggaaaac ataaataatt
     4681 gcgtcgcacg cgtcctgcgt gatacagtca aattcggccc cgcctcgcgc ctcatccggc
     4741 cacgtcaacc gaccgcctcc gtgacagtca gccatcaggg tgtaatgaaa cgcacgcaag
     4801 acaagattaa ggacatcgtc gagccgcagg acttcgacga ggtgcgtaac gtcgtctccg
     4861 accccgcacg ctctctcgcc gcctactgct tcaccgacgc cacttccgac ctcctcgcgc
     4921 gctggctcga cgccttagcc gacctcccgc gcggacgcgg ggcggcgcgc tcgctcgcgg
     4981 gcgtccgggg cgtcggcaag agccacgcgc tggccgtctt cggcgcgctg gccgcgcagg
     5041 agcggctgcg tctggacgtg tcggacgcgc acgtggcgac gagcgcgcgg cgtctgtcgg
     5101 gccgacgcta catggtcgtc cgcgtcgagc gcggcacgcg cgacacgctc gccgaggaga
     5161 tggccgccgc tttcggccgg gccttcggag ggagcgaagc gcagtggggc actgacccgg
     5221 ccgggatgct ggccgtcgcc ggctcgcgcg cccacgactc gacgctcgtc ctcatcatcg
     5281 acaccggctt cgaccgcccc tcgcgcgtcc ggcgcgacga cggcacgcac ctcggcgaga
     5341 tcgcccgcac cgcccgcggc gccaacgcct tcgtcgccct cgccctcgac gacgacatct
     5401 ccggcgccga cggccccaac gtcgccatcg ccggcaccta tcacatcgac ttcctcgacc
     5461 acgaacatct cttccgcatc gccgacacct acatcctcca caagaacgag cggggccgcg
     5521 ccgcgctcca cgacatctat ctccacctgc gcgcgagtgt gcccggcttc aactggagcg
     5581 agccgcgctt cgccgcgctc taccccgtcc acccgctcgt ggccgacgtg gcggcggccg
     5641 tgcgtctcta cgcgcccaca tttgcttttc tccccttcgc cgccgaggcc gcacgccgcg
     5701 ccacgtcccg gcccgcgctc tcgctcgtcc tgctcgacga ggtcttcgac cgcgccgagc
     5761 gcgacctgcg caagtcgccg gagctgaagg aggctttcga ggcttacgac gatctggcga
     5821 cgcgcgtcgt cgcgcagttc cccgtcatgc agcggctgga ggcgaagctc gtcttaaaga
     5881 cgctcttcat cctctcgctc gacggcggcg gcgccacgcc gcaggggctg tgcgcggcgc
     5941 tgctggtctc gcaggagtcg gcgcccgcgg gcggcgcggc ccgcgtgagc gagatgctcg
     6001 acaggttcgt cgaggccgcg cccgaagccc tgcgtgttgg cgacgaggcg gcggccggcg
     6061 acggcccacg ctatcgcctc cagatcggcg ccctcgacaa attccagacc gcgcttgagc
     6121 gcagcgtcga gcgcctgccc gacggctccg actccctcgc gctcacgcag ctcagggccg
     6181 cggcccacgc gcgcttcgag gactggccct tcgccgacgg ccacgatgag cagacggcga
     6241 ccgccaactt caaccttgcc tggcgcggct ccgagcggcg cgcgcgcatg gtctgggttg
     6301 acgcggcggc atcgccaccc gccgccgcgc ccgacttcga cctcgaagtt tttatctatg
     6361 cgcccggcgc cgaagccacg cccgcgccgc cggccgccac agatgaggat gccggcgcgg
     6421 cgccgctctc ggtcgtctgg cggcccggcg aggtcacagc ggaagagttg acgacgctgc
     6481 gccgactcct cgccctgcgc acggacggca cgctcgccgc ggacttcacc gagacggccg
     6541 tcgccgccga gacggtgctc gccgcgcagg cccagcgcgt ctgggcgcgc atctacctcg
     6601 acgcgggcac gctcgaagtg ggcggcgagc ggaagtcgcc cgacgaccgc gcgcgcggct
     6661 cgcgcacgct ctcggccgcc ctctccacga cgctcgcgcc acacttcgcc gaactctacc
     6721 cgcaacaccc acagttcgcc gagacacttc gggacgcgga cgtagagcgt ctgtcggctg
     6781 gcctcttcgc cggcgccggc gcatccgacg cggaggtcga gcgtctggcg cgcgtcttcg
     6841 cgcttccgct cggactcgtc gagcggcgcg gcaacgagca catcctcgcg ccgggcgacg
     6901 acggcgcgga cgaccccgcg tgggtgaagg aagtgctccg actcgtcgct cacactgacg
     6961 ccgcgtcagc cccgctcaca tcagcgtcgc ccgcaacagt cccgctcgaa tcaatccgcg
     7021 gcgctctccg ccgtccgccc tacggactcc agcgcgaggc gcagaacctc ttgctcgcgg
     7081 cgctcgccgc gcagcgacgc atcgaactct tcgacgcgtc gggcgagcgc gccctccgcc
     7141 ccggccgccc gctgaactgg gatgaagtcg cctcggtcgc gcccgcggcc caacagggtc
     7201 gcgacccgga gcagtcggcc gcgtgggcgc gtctactgac ggggcggccc gacctccgtg
     7261 cgctgtccga tagggaggcg cgcggcgccg cctcgatggc gctctccgag tggctcgaac
     7321 gctggcgcgc actcaaactt ggcgagcgtt tcgacgcgct gcccgacgcg ctgttgacga
     7381 cgcgcgtgtg gagaatgaat gcggaggcgt gccggcgctt cggggcggcg gccgaggccg
     7441 tccgcctcgt gctcgccagc gaactctcgc tcgacgaggg gctggagcgc gtggccgacg
     7501 ccttcgacaa ttccgccgac acgctcgccg aggccgcgca cgacctcgac gcgctcaccc
     7561 agttcgtgga aaattgcgtg gcgcgccgcc gcgccgccgc ttatctcctg acggcggggc
     7621 agaccggcgt ggacgagatc gaggccgcgc gccgcgagct gctttcaatc gcgcgggagc
     7681 gcgcctgcct gttcgacgcc gagcgcaacg aacgcttcaa ccgcctctgg cctgagttcc
     7741 agacgctcta cgcggagcac tatgcggccg cgcacgacgc ggccggcgcg cacacccggc
     7801 gcgcgctcga cacgcttctg gccggcgacc gctggcgcga gttcgagatg ctttcgcggc
     7861 tgcccttcgt gacgcggcag tattgggagg cggcccgcga gctgctgcgc cgcgccggcg
     7921 cgccgcgttg cgatctgccc gtgcggcagt tgctcgccgc gcgcactgcc tgcgcatgcg
     7981 gcttcaagct ctcgggcgcc gacgcggcgt cgcggctgcc gcgggagttt gaagaagttt
     8041 tggagtcggg gctggccggc taccggcgca cgctcgcgct cctcgccgcg ccgctgcgcc
     8101 gcgcgctcga agcactggcc gggcgcgagg aggagcggcg cgagacggcc acgcgcgcac
     8161 gcatcctcgc cgcggccttc ggcgcgggca cgccgccgcg tcgcttctcg cgcgccgacg
     8221 cgcaactgat cgaacgcgcc gccgccgaga tgggcgcacc gccctccgtc cgcgccagcg
     8281 tgccgacgga tggcggccaa ctgctcacgc gcgacgaact ctccgcgcgc ctgcaacagt
     8341 ggctcgacga cctgccggcc aacgccgcgc tcgtcgaact cgtcgccggc ggcagcgaaa
     8401 cacatgcctg acagaaaagc ggcgaagggg aaagcgggaa gggggaaggg aagacgaggg
     8461 acgggggaca ggggacgagg gaggaagaga actgttctcc cttccccttt accccttccc
     8521 ctttgcccct tcccccgcct gtccatcgcg gtcgtcggcg cgggccgact cggcacggcg
     8581 ctggctcgct cgctcgccgc cgcgggttgt gagatcgacg cggtcgtcgc acgccgcatc
     8641 gcacgggcgc gacgcgccgc cgcgctcatc ggcacgaaag cgcccgccct ctccgccgcg
     8701 caactagaac gcctccccaa agtcgatctg ctccttctga cgacgcctga cgacgcgctg
     8761 ccggaagtgg tcgcgcgcct tgccgcgctc gggaaagatg acgagacgcc ggcgcgtccg
     8821 aaagtgggcg cgcggagtcg cggcgcgggc gcggtggctc tgcacgcgag cggcgcgctc
     8881 gggtcggaag tcctcgcgcc cctgcgcgag cgcggctacg cggtcgggtc gctccacccg
     8941 ctcgtctccg tgagcgacgc acgagccggc tccgaaatct tccgcggcgc ctactactgc
     9001 gtcgagggcg acccggcggc agtgcggctc gcgcgccgcc tcgtgcgcgc tctcggcggg
     9061 cagagtttca cggtcgcgac cgaccggaag gcgctctacc acgccgcggc cgtgatgacc
     9121 tccggccaca ccgtcgcact cttcgacacg gccgcgcgcc tcctcgcgct gtgcggcctc
     9181 acacccgcgc gcgcccgcga agtgctcctc cccctgctcg gcagcacgtt tgagaacctc
     9241 tccgccctgc ctccaccgcg cgccctcacc ggcacctacg cccgcgccga cgccgacacc
     9301 gtccgcaaac acctctccgc cctcgccgac gccggcgacc acaacgctct taccgtctac
     9361 acgcttctcg cacggcactc cttactgctc gccgaagcaa acggtgctga cccgacgagc
     9421 ctcaaagaaa tcacgcgcct gctagagcga tattcacttg agtggaagcg cagttgattc
     9481 accgcggagt cgcagaggac gcggagttcg cgcagagtgt tgaatcgctc tgcgcgaac
//
LOCUS       DASVES010000481         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1258888, whole genome shotgun sequence.
ACCESSION   DASVES010000481 DASVES010000000
VERSION     DASVES010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258888"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF240_20165"
     CDS             <1..207
                     /locus_tag="VF240_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177585.1"
                     /translation="PAHVQTLQNLTVALTAKGDAAGARATLAKLESASPQNPSLPRLR
                     ADLEKFSATGQATAKTTAAAEGGK"
     gene            complement(347..1036)
                     /locus_tag="VF240_20170"
     CDS             complement(347..1036)
                     /locus_tag="VF240_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177586.1"
                     /translation="MSDLLRAIRDLEKFTGHSLTRRIETLESALSGTDADAHAAILAN
                     ESISRELLAGAYLLKRKAGQINVVIHALGILLLIPHIIEPGERIKYLSLGAGNTGKPF
                     DLETTHRVAEFTFINWRGRADTGRQNKLFKDFFLLAEHPTPKRKYLYVLGTEHPLRFL
                     TSRRSPESVMSRNNPLWADFQSQYNDRFSTVGQYFAHRHQAVTIEDVGKYLPELFMSE
                     DITSTDDEGAL"
     gene            complement(1072..2103)
                     /locus_tag="VF240_20175"
     CDS             complement(1072..2103)
                     /locus_tag="VF240_20175"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEX7177587.1"
                     /translation="MIGNTPLLEISLRFRGQPRTVYAKAEHLNMTGSIKDRMAFHILK
                     SAYREGRVRPGDVIAEATSGNTGISFAAIGRALGHEVRIYMPDWMSRERVALIRSLGA
                     RVHPVSREQGGFLGSIRMAEELAAREPHVFLPRQFSNAANVEAHEQTTAPEIWWQLHS
                     HSLRPDAFVAGVGTGGTVMGVGRYLRRHNPAVRLHPLEPAESPTLSTGHKTGRHRIQG
                     VSDEFIPPILELGTLDEVVSVHDGDAILMAQKLGTELGLAVGISSGANFLGALVVQDE
                     LGAGSVVATVFPDDNKKYLSTDLLGDEPVRAGYLAPDVELLSYRAFGRVCYTCFEPDA
                     PDVRLLKSA"
     gene            complement(2273..2863)
                     /locus_tag="VF240_20180"
     CDS             complement(2273..2863)
                     /locus_tag="VF240_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177588.1"
                     /translation="MRTVKLLAVTLMLALAGAVYAAGAMQDAAKPQGETKAAKSCCQM
                     HGKAKAADAHAGMKHEGEGCCGGGSGCCAGRACAKEHKGDHAAATQKTAAADEKAGCC
                     SGDTGCCAGGACVKGHKDAKTQAVAMSGESCCKEGAACCKKGATCCKTHKAAAGQTAA
                     KAVDAKGAGAASCCGGGGSCACCGDKDSHKTHAGGQ"
     gene            complement(2948..>3146)
                     /locus_tag="VF240_20185"
     CDS             complement(2948..>3146)
                     /locus_tag="VF240_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177589.1"
                     /translation="SCCGKSRQPSDAARKLRPATDGATTNTRAECSHRDDGAFDVARP
                     AARSRSPDGRSAHVRCCVFVI"
BASE COUNT          514 a         1025 c         1029 g          578 t
ORIGIN      
        1 cccgcgcacg tccagacgct ccagaacctc accgtcgcgc tcaccgccaa gggcgacgcg
       61 gcgggcgccc gcgcgaccct cgccaagctc gaatcagcca gcccgcagaa cccctccctc
      121 ccgcgcctgc gcgccgacct cgaaaagttt agcgcgacgg gccaggcaac ggccaaaacc
      181 accgccgccg ccgagggcgg caagtagacc ctctcgaaaa aagcaagaag cgacaacagc
      241 cctcgaagca cgaagcccgc gacctgaatc gcgggcttcg tgccatacct ggttaacgac
      301 tgaaacaatc gttctcacag atggtcgtta gacggccggc gcggaactat aacgctccct
      361 catcatcagt gctcgtaata tcctcagaca tgaacagctc ggggagatat ttgccgacat
      421 cctcgatggt tacagcctga tgtctgtggg cgaagtattg gcctaccgta gagaagcgat
      481 cattgtactg actctggaag tcggcccaca gtggattgtt acggctcatt acgctctctg
      541 gcgaccgtct gcttgttaaa aaccgtagag ggtgctccgt gccaagcacg tagaggtatt
      601 tccgtttcgg cgtcgggtgc tcggcaagca gaaagaaatc tttgaacaac ttgttctggc
      661 gaccagtgtc ggcgcggccc cgccagttta tgaacgtgaa ctcggcgacg cggtgtgtgg
      721 tttcaaggtc aaacggcttt ccggtattac ccgcgccaag cgagaggtac ttgatcctct
      781 ctcccggctc gatgatgtgg gggatgagga ggagtatgcc gagcgcgtga atcaccacat
      841 tgatttggcc ggccttgcgc ttcagcaggt aagcgcccgc gagtaattcg cgcgagatcg
      901 actcgttagc gaggatggcg gcgtgagcgt cggcgtcagt gcccgagagc gcggattcca
      961 gagtctcgat tcgccgcgtc agactgtggc cagtgaattt ttcgaggtca cgtatggcac
     1021 gaagtagatc gctcacaggc agcctccgca tcgcgcccgg cgagcgagtc ttcaggccga
     1081 ttttaacagt cgcacgtcgg gtgcatccgg ctcgaagcac gtatagcaca cgcggccgaa
     1141 ggcgcggtag ctcaaaagct ccacgtcggg cgcgaggtag ccggcgcgca caggctcgtc
     1201 gccgagaagg tctgtgctca ggtacttctt attgtcgtcc gggaagacgg tggcgacgac
     1261 ggagccggcc ccgagttcgt cctgcaccac gagcgcgccg aggaagttgg cgcccgagga
     1321 gatgccgacg gcgaggccga gttcggtgcc gagcttctgc gccatcagaa tcgcgtcgcc
     1381 gtcgtggacg ctgacgacct cgtcgagcgt tccgagttcg aggatgggcg ggatgaactc
     1441 gtcggagacg ccctggatgc ggtggcggcc cgtcttgtgg ccggtcgaga gcgtcggcga
     1501 ctcggccggc tccagggggt gcaggcggac ggccgggttg tgccggcgca ggtagcgccc
     1561 gacgcccatc accgtcccgc ccgtccccac tccggcgacg aaggcgtccg gccggagcga
     1621 gtgcgagtgg agttgccacc agatttccgg cgcggtcgtc tgctcgtgtg cctcgacgtt
     1681 ggccgcgttg gaaaactgtc gggggaggaa gacgtgtggc tcacgcgcgg ccaactcctc
     1741 ggccatgcgg atgctgccca ggaagccgcc ctgctcgcgg ctgaccgggt ggacgcgcgc
     1801 gccgaggctg cggatgagcg cgacgcgctc gcggctcatc cagtcgggca tgtagatgcg
     1861 tacttcgtga ccgagcgcgc ggccgatggc ggcgaaagag atgccggtgt tgccggaggt
     1921 cgcttcggca atcacgtcgc cgggccggac gcgcccctcc cggtaggcgc tcttcaaaat
     1981 gtggaacgcc atccggtctt taatgctgcc ggtcatgttg aggtgctcgg ccttggcgta
     2041 gaccgtgcgc ggttggcccc tgaagcggag gctaatctcc agcagcggcg tgttgccgat
     2101 catgtgcttc aactctccga agcgctcagt cggtcggcac ggcgctaagg caacctggtt
     2161 cacggcctgc tcctttgacc tgcgggcggc gggggttgtg gctgtctttc aaacgaccta
     2221 aagcggaggg gcgcgagtcg aaactcgcgc ccctccgctt gcggggtctc actcactgcc
     2281 cgccggcgtg cgtcttgtga ctatccttgt cgccacagca cgcgcatgag cccccgccgc
     2341 cgcagcaact cgccgcgccc gcgcccttcg cgtcaacggc cttggcggcc gtctgtccgg
     2401 cggcggcctt gtgcgtcttg cagcaggtcg cgcccttctt acagcaggcg gcgccttcct
     2461 tgcagcaact ctcgccgctc atcgcgacgg cctgcgtctt ggcgtctttg tgtcccttga
     2521 cgcaggcgcc gccggcacag cagcccgtgt cgcccgagca gcagccggcc ttctcgtccg
     2581 cagcggcggt cttctgcgtg gccgcggcgt gatcgccctt gtgttctttg gcgcaggcgc
     2641 ggccggcgca gcagccgcta ccgccgccac agcaaccttc gccttcgtgc ttcatgccgg
     2701 cgtgggcgtc ggcggccttc gccttgccgt gcatctggca gcagctcttt gccgccttcg
     2761 tctcgccttg gggtttcgca gcgtcctgca tcgcgcccgc ggcatagacc gcgccggcga
     2821 gtgcgagcat gagggtgact gccaaaagtt taacggttct cattatcctt ctcctgattc
     2881 tccctagcgg gtttccggaa agacaaccgg cgagtgcgcc cgccggcgtg ttattcgaga
     2941 cccccgccta gatgacgaac acgcagcagc ggacgtgggc cgagcgcccg tcaggcgagc
     3001 ggcttcgggc tgcgggccgt gcgacatcaa acgcgccgtc gtccctgtgc gaacactctg
     3061 cacgcgtgtt cgtcgtggcc ccgtcagtcg ccgggcgcag cttgcgcgcg gcgtctgaag
     3121 gctgacgcga cttcccgcaa cacgag
//
LOCUS       DASVES010000482         7269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_21907, whole genome shotgun sequence.
ACCESSION   DASVES010000482 DASVES010000000
VERSION     DASVES010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21907"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..3514)
                     /locus_tag="VF240_20190"
     CDS             complement(134..3514)
                     /locus_tag="VF240_20190"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177590.1"
                     /translation="MSRFNRLRLKGSAPLVALVALYALLACALPHPARAQQQPQPRTP
                     DAQTQQSGAARNPFRTLRYRQIGPFRGGRVTAVAGVTSQPLVYYFGATGGGVWKTTDG
                     GSNWEPVSDAFFKTGSVGAIGVCESDPNVVYVGMGESPIRGNVSHGDGVYKSTDAGKT
                     WKHTGLSDTRQIGRVRVHPRNPDLVYVAALGHTWGPNAERGVFRSKDGGKSWEKILFR
                     GDKAGAIDLTFDPTNASVLYAGFWEVYRKPWTLESGGAAGGLFKSTDGGDTWTELTRN
                     PGGPRGVVGKIGVTVSPVNPERVWAIIEAEDGGVFRSDNGGRTWTRVNEQRNLRQRAW
                     YYTRIYADTQNPESVYVLNTGFYRSNDGGRTFSNISVPHGDNHDLWIAPNDAQRMINA
                     NDGGANVSVNGGRTWTEQDQATAQFYRVTTDNDFPYHVYGAQQDNSTVKIASRTEGFA
                     ITESDWHDVGGGESGWIAPHPADSNVVFAGSYGGYITRYDHRTRQLRTVNVWPDNPMG
                     AGAETMKYRFQWNFPILFSPHDPNTLYAAGDRLFRTTNEGQTWEAISPDLTRNDRSKQ
                     GSSGGPITKDNTSVEYFDTIFTAAESSVTRGVIWAGTDDGLVQLTRDAGKTWANVTPQ
                     GMPEWIQVNSIEASPHEAGAAYVAATAYKLDDNRPYLYKTSDFGKTWKRITGGIAADA
                     FTRVVREDPHRRGLLYAGTETGMYVSFDDGDSWRPFQLNLPVVPITDLAVHKRDKDLI
                     VATQGRSFYILDDLTVLHQMTPAMLAATNETRLLKPEDAYRTQGGGGNPLPLTTPVGQ
                     NPPNGVVVHYTLKARPTTDVTLEFLDSTGKSIRKFTAKAPARPTPSPTPAAVSGVVPS
                     PGMPQTPGVAQVTTPPEQPQAPSEEASEFAPGGGAGGGPRVSVEPGLNRFVWDMRYAE
                     ATRFPGMILWAANTQGPRVVPGTYQVRLTADGQTFTESFEVKRDPRLATTPQEFARQH
                     ELLLRVRDKLTETHDLIIQLRDVRQQVDELTKRVADQPEAKQVVERGKELMSKLSAVE
                     ETLYQTKVQSSQDTLNFPIRLNNKLASLASGVASSDSPPTDSSVALADELTTQIDAQL
                     ARIRQLLDADLKAYNQIVRAADIPAVIMKPKPAPAGGQ"
     gene            4589..5398
                     /locus_tag="VF240_20195"
     CDS             4589..5398
                     /locus_tag="VF240_20195"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX7177591.1"
                     /translation="MRLLTSESPAADVRAGALGRARAGLYYVVLLALLAPGIYEGVRQ
                     YRETQYMRAVALEATRESAPGDARALIVALRDYVRRQVRHEEHYISRDDRPFLRASAA
                     DTLRSGKGDCGEATRVFINLAGAMGVRAQRLYLEGQQPHVVAEVRLGDTDVLVDSYDA
                     PFVPELATLEQVLRRPEFDHYSSLNWRRLLLGLPSFKINLGPLAYLLENPHALTALLW
                     FALASVWASLRFVRLPGAHRLLRRKGEGLHHEHRVSRTLRPAVRSGQKAKD"
     gene            5837..>7269
                     /locus_tag="VF240_20200"
     CDS             5837..>7269
                     /locus_tag="VF240_20200"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7177592.1"
                     /translation="MTTRRDFLKTASVAAGAATLLPAWVYEAEAWAADTPVDKMRLAD
                     VALSTAKRAGASYADIRINRYRFEAVSTREQQVQNVSRTQSFGFGVRVLYKGTWGFAA
                     SRDVSDAEVRRVTLSAVEIARANSAFQRKPVRLVPTPKVVAKWKSSFERDPFDVPLDE
                     KIAFLLKLNETAMKTKGVSFVNSSMTWVNEQKFLATSDGSRIEQYIIRGNPDFTVTAV
                     GGGDFQTREAFAGPQAMGYEYMDKYPWLVEAEAAGAEAVAKLSAKSIEPGRYDLVLHP
                     SHLFLTIHESVGHPTELDRALWWEANYAGTSFLTPEKTGKLQFGSKLVNFFADRTQPA
                     GLATVGYDDEAVPGQKWYLVKDGVFVDWQTTRDLAPLIGRKKSYGCLHAQSWSDVPFP
                     RMPNVSLEPAKENVSLDDLVGGVDNGIMIHGRGSYSIDQQRYNFQFGGQTFWEIKKGK
                     ITGMLRDVAYQARTTDFWNSLDALGGPA"
BASE COUNT         1206 a         2350 c         2348 g         1365 t
ORIGIN      
        1 ttataggact ccgcaccggc tttggttttg acacagtcaa aacagaattc cgacgaatat
       61 cgtgtaaaaa agttttcgat tatcgtgttt gcgcgcaatg ggctacattc tgccgcccct
      121 ccggggcttg tctttactgc cccccggccg gcgcgggctt cggcttcatg atgacggccg
      181 ggatgtcggc cgcgcggacg atttggttgt aagccttgag gtcggcgtct aaaagttgcc
      241 ggatgcgcgc gagctgcgcg tcgatctgcg tcgtgagttc gtcggccagg gcgacggatg
      301 agtcggtggg cggcgagtcg gagctggcca ccccgctcgc cagcgaggcg agcttgttat
      361 tgaggcggat ggggaagttc agtgtgtcct gactgctctg caccttcgtc tggtagagcg
      421 tctcctccac ggccgacagt ttcgacatca actccttgcc gcgctcgacg acctgcttcg
      481 cctcgggctg gtcggcgacg cgcttcgtca gctcgtccac ctgctgacgc acgtcgcgca
      541 gttggatgat caggtcgtgc gtttcggtga gcttgtcgcg aaccctgagc agcagctcat
      601 gctggcgcgc gaactcctgc ggcgtggtcg ccagccgagg gtcacgcttc acttcaaaac
      661 tttcggtgaa ggtctggccg tcggccgtga ggcgcacctg atatgtgccg gggacgacgc
      721 gcggcccctg cgtgttcgcc gcccagagaa tcatgccggg gaagcgcgtg gcctccgcgt
      781 agcgcatgtc ccagacgaag cgattgagtc ccggctcgac gctcacgcgc ggccccccgc
      841 ccgcgccgcc gccgggggca aactccgaag cctcctcgct cggcgcctgc ggctgctcag
      901 ggggagtcgt gacctgtgcg acgccgggag tctgcggcat gccgggcgac gggacgacgc
      961 ccgagacggc cgcgggcgtg ggcgaaggcg tcggccgcgc cggcgccttc gcggtgaact
     1021 tgcgaatcga cttccccgtc gagtctaaaa actccagcgt cacgtccgtc gtcgggcgcg
     1081 ccttcagcgt gtagtggacg acgacgccgt tcggcgggtt ctgcccgacg ggcgtcgtga
     1141 gcggcagcgg gttgcctccg ccgccctgcg tgcggtaggc gtcctcgggc ttgaggagcc
     1201 tcgtctcatt cgtggcggcg agcatcgcgg gcgtcatctg gtggagcacc gtgaggtcgt
     1261 cgaggatgta gaaggagcgc ccctgtgtgg cgacgattaa atccttgtcg cgcttgtgga
     1321 cggcgaggtc ggtgatgggg acgacgggca ggttcaactg gaacggtcgc cacgaatcgc
     1381 cgtcgtcgaa ggagacgtac atgccggtct ccgtgccggc gtagagcaat ccgcgccggt
     1441 gcgggtcttc acgcacgacg cgcgtgaaag cgtcggcggc gatgccgccc gtgatgcgct
     1501 tccacgtctt gccgaagtcg ctcgtcttgt agaggtaggg ccggttgtcg tctaacttgt
     1561 aagcggtcgc ggcgacgtag gccgcgcccg cctcgtgcgg cgacgcctcg attgagttga
     1621 cctgaatcca ctccggcatc ccctgcggcg tgacgttcgc ccaggtcttg ccggcgtcgc
     1681 gcgtcaactg caccagcccg tcgtcggtgc cggcccagat gacgccgcgc gtgacgctcg
     1741 attcggcggc cgtgaagatg gtgtcgaagt actcgacgct cgtgttgtcc ttcgtgatgg
     1801 ggccgcccga ggagccttgc ttcgagcggt cgttgcgcgt caggtcgggc gagatggcct
     1861 cccacgtctg cccctcgttg gtggtgcgga agagtcggtc gccggccgcg tacaaagtat
     1921 tcgggtcgtg cggcgaaaac aggatgggga agttccactg gaagcgatac ttcatcgtct
     1981 cggcgccggc gcccatcggg ttgtcgggcc agacgttgac ggtgcgtagt tgccgcgtgc
     2041 ggtggtcgta gcgcgtgatg tagccgccgt aggagccggc aaagacgacg ttggaatccg
     2101 ccgggtgcgg cgcgatccag ccggactcgc cgccgcccac gtcgtgccag tcagattccg
     2161 tgatggcgaa gccttcggtg cggctggcga tcttcacggt cgaattgtcc tgctgcgcgc
     2221 cgtagacgtg gtaggggaaa tcgttgtcgg tcgtgacgcg gtagaactgc gccgtcgcct
     2281 gatcctgctc cgtccacgtg cgcccgccgt tgacggagac gttcgccccg ccgtcgttcg
     2341 cgttgatcat gcgctgcgcg tcgttcggcg cgatccacaa atcatggttg tcgccgtgcg
     2401 ggacggagat gttcgagaac gtgcgcccgc cgtcgttgga gcgatagaag ccggtgttga
     2461 ggacgtagac ggattcgggg ttctgcgtgt cggcgtagat gcgtgtgtag taccacgcgc
     2521 gctgccgcag gttgcgctgc tcgttgacgc gcgtccacgt gcgcccgccg ttgtcggagc
     2581 ggaagacgcc gccgtcctcg gcctcgatga tcgcccacac gcgctccggg ttgacgggcg
     2641 agacggtgac gccgatcttg ccgaccaccc cgcgcggccc gccggggttg cgcgtcagct
     2701 ccgtccacgt gtcgccgccg tccgttgact tgaagagacc gcccgctgcg ccgcccgact
     2761 ccagcgtcca cggcttgcgg tagacctccc agaagcccgc gtagagaaca ctagcgttcg
     2821 tcgggtcgaa cgtgaggtca atcgccccgg ctttgtcgcc gcggaagaga atcttctccc
     2881 aactcttccc gccgtccttc gagcggaaca cgccgcgctc ggcgttcggc ccccacgtgt
     2941 gcccgagcgc ggcgacgtag acgagatcgg ggttgcgcgg gtgcactctc acgcggccta
     3001 tctgccgcgt gtcggagagc cccgtgtgct tccaggtctt accggcgtcg gtggacttgt
     3061 agacgccgtc gccgtgtgag acgttgccgc ggatgggcga ctcgcccatg ccgacgtaga
     3121 cgacgttcgg gtccgactca cacacgccga tggcgccgac cgagccggtc ttaaagaacg
     3181 cgtccgagac aggctcccag ttggagccgc cgtcggtcgt cttccacacg ccgccgcccg
     3241 tcgcgccgaa atagtagacg agcggctgtg acgtgacgcc cgccacggcc gtcacgcgcc
     3301 cgccgcgaaa cggcccgatc tgcctgtagc gcagcgtgcg gaaaggattc ctcgccgccc
     3361 ccgactgctg cgtctgagcg tcgggcgtgc gcggctgcgg ctgctgttgt gcgcgcgccg
     3421 ggtgtgggag cgcgcacgcg aggagtgcgt agagcgcgac gagcgcgacg agcggggcgc
     3481 tgcccttcag ccggaggcgg ttgaagcgtg acataaggtt aggctccttc gattcttttg
     3541 tcttcgagct ttgacgaacc tgcgtgtgga tgttgccaac tgtgccgcac actatattct
     3601 ttcgcccccg ccgaattcaa cctgaccccg cgatagcctc acttctacct cagtcgcatc
     3661 tggcttcgcg gccagataca aaagcgttga ctaaagcctg ttcttcagta caatgcgggt
     3721 cgtcggcctc tgttaacaag acgatttacg ctcagcccgt caaccagctt tgcgacctca
     3781 caaagtggtt acttacctgt cgtggcaaag cgtccgatct ttccacctca acctgtgaag
     3841 gagaaatgaa atgtccaccc aaaccaaact ccgcttgaaa aggcgcaccc gtgttgctct
     3901 gcttttcgtg acgatgatct tgcttttggg cacgtccacc gcacccccac cggcatgcgc
     3961 tagtagcttc tgcctgtggc gcgcgagagt gcacttttac acggatgcca gcaagacgac
     4021 tctgtgcggc ttttcgaact attcttgtgc cggcaatttc acacacacag ggtgctacac
     4081 ggactactac gtgataacag agtgtgagtg caaccaatag gcacgattta aactgagacc
     4141 agatcgcacg gttaggccaa gcagccgccc cggtagcaaa cggcaaggcg ggcgttatcc
     4201 gtcggctgtt aggcttagtg tgtccagggc ggcaagcaga caactcgcta ctaaaagggg
     4261 cgaagcccga aagcctcgcc cccttatatc ttcccgtcct caaagttccc gctaacacct
     4321 gacatcatgt ttgaactgca acactgatta agatacgttg tagcactcca gcggcagcat
     4381 gcacccgacc cgcaattagc gactctctta agcctcggcg ggtgtagacc aagcacctga
     4441 cagaaatggg cgtggaagac gaagcgaggt tcatcctgtc gcaagttggt tgggaaggcc
     4501 attcgcggcg cgggcgcact tccgcgcccg gcgccccgtg ggacggctat cgtaaaagat
     4561 tcgctcttga tatggtgttc gacgacgtat gagacttttg acgagtgagt cgccggcggc
     4621 cgatgtccga gccggcgctc tcggccgggc acgcgccggc ctttattacg tcgtcctgct
     4681 ggccctgctt gcgccgggaa tttacgaggg cgtgcggcaa taccgtgaga ctcaatacat
     4741 gcgtgccgtg gccctcgaag cgacgcggga gtcggcgccg ggcgacgcgc gcgcgcttat
     4801 cgtggcgctg cgcgattacg tccgccggca ggtgcggcac gaagagcatt acatctcgcg
     4861 ggatgatcgc cccttcctgc gggccagcgc ggccgacacc ctgcgctcgg gcaagggcga
     4921 ttgcggcgag gcgacgcggg tcttcatcaa tctggccggc gccatgggcg tccgcgcgca
     4981 gcgtctctac ctggaggggc agcagccaca cgtcgtggcg gaggtgcgtc tgggcgacac
     5041 ggatgtgttg gtcgattcct acgacgcccc tttcgtcccg gagctggcga cgctcgaaca
     5101 ggtgctgcgc cgccccgagt tcgaccacta ctcgtcgctc aattggcggc gcctgctgct
     5161 cgggctgccg tcctttaaaa tcaatctggg gccgctcgcc tatcttctgg agaacccaca
     5221 cgccctcacg gccctgctct ggttcgcgct cgcgtccgtg tgggcgagcc tgagatttgt
     5281 tcggttgccc ggcgcgcatc gtctgctgcg tcggaagggg gaaggccttc atcacgagca
     5341 cagagtatcg cgcacgcttc ggccagccgt aaggtcaggg caaaaggcaa aagattaagg
     5401 gccgagccgc aggcgtcgtc gccgcaggcg acaacgagcc ccggagcggc ggcagcagtg
     5461 ttgccccggt cgtgagaccg gggaacggca gctgaaaatt cacccgaagc cccggagggg
     5521 cgaaagaatc tccacgctgc acgtcgcggc ccgggaaatt ctttcgcccc tccggggctt
     5581 caccatttat cgcggactgt tcccacggct cacgccgtgg gctacaatct gccgccgcct
     5641 ccggcggctc gtcggcgcct gcgactcggc tctttaactt ttgccttttt ccttgtgcct
     5701 tcctccaacc ctcgctgaaa agtctcttga cttttcgtcc cgctgtaata aagttacagc
     5761 ctccagtcat tcaaagttat tccgaaacag tccgaaacgg gcgcgctccg atgcgccgga
     5821 ctcgcgaggt cttcacatga caactcgacg cgacttcctg aaaacggcga gcgtggccgc
     5881 gggcgcggcg acgctgctgc ccgcctgggt ctacgaggcc gaggcgtggg ccgccgacac
     5941 gcccgtggac aagatgcgcc tcgccgacgt ggcgctctcg accgccaaac gcgctggcgc
     6001 cagctacgcc gacatccgca tcaaccgcta tcgcttcgag gcggtctcga cgcgcgagca
     6061 gcaggtgcag aacgtctcgc gcacgcagag cttcggcttc ggagtgcgtg tcctctacaa
     6121 aggcacgtgg gggtttgcgg cgagccgcga cgtgtcggac gcggaggtgc gccgcgtcac
     6181 gctgtcggcc gtcgagatcg cccgcgccaa ctccgccttc cagcgcaagc ccgtccgcct
     6241 cgtccccacg ccgaaggtcg tcgccaaatg gaaaagctcg ttcgagcgcg acccgttcga
     6301 cgtgccgctc gacgagaaga tcgctttcct tctgaaactc aacgagacgg cgatgaagac
     6361 caagggcgtc tccttcgtca actcgtcgat gacgtgggtc aacgagcaga agtttttagc
     6421 cacctccgac ggctcgcgca tcgagcagta catcatccgc ggcaaccccg acttcaccgt
     6481 gacggccgtc ggcggcggcg acttccagac gcgcgaagcc ttcgccggcc cgcaggcgat
     6541 gggttacgag tacatggaca agtacccgtg gctcgtcgag gccgaggcgg cgggcgcgga
     6601 ggccgtcgcc aagctctcgg ccaaatccat cgagccgggc cgctacgacc tcgtgctcca
     6661 cccgtcgcac ctcttcctga ccatccacga gtcggtcggc caccccaccg aactcgaccg
     6721 cgcgctctgg tgggaggcca actacgccgg cacgagtttc ctcacgcccg agaagaccgg
     6781 caagctccag ttcggctcga agctcgtcaa cttcttcgcc gaccgcacgc agccggcggg
     6841 gctggccacc gtcggctacg acgacgaggc cgtgcccgga cagaagtggt atctggtcaa
     6901 agacggcgtc tttgttgact ggcagacgac gcgcgatctg gcgccgctca tcggacgcaa
     6961 aaagtcttac ggctgcctgc acgcgcagag ctggtctgac gttcccttcc cgcgcatgcc
     7021 aaacgtctcg cttgagccgg cgaaggaaaa cgtctcgctc gacgatctgg tcggcggcgt
     7081 cgataacggc atcatgatcc acggacgcgg cagctattcc atcgaccagc agcgatacaa
     7141 cttccagttc ggcgggcaga ctttctggga aatcaaaaag ggcaagatca cggggatgct
     7201 gcgcgacgtg gcctatcagg cgcgcaccac cgacttctgg aactcgctcg acgccctcgg
     7261 cggcccggc
//
LOCUS       DASVES010000483         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1066584, whole genome shotgun sequence.
ACCESSION   DASVES010000483 DASVES010000000
VERSION     DASVES010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066584"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VF240_20205"
     CDS             <1..1416
                     /locus_tag="VF240_20205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7177593.1"
                     /translation="VTSGNERVFCAGANIPMLATSTHAFKVNFCKFTNETRCAIEDAS
                     RNSNQRYLAALNGTASGGGYELAIACDEIYLVDDGNSAVSLPEVPLLGVLPGTGGLTR
                     LVDKRKVRRDRADVFSTLAEGLKAKRATEWGLIDGYFATSKFQEGVEARVGEIVAAGD
                     ASLPGPGIKLNPLEVETSETGREYKYVSVRLNREGRYADLTMRAPVGGLPASADEAVS
                     LGDSFWALRAYRELDDALLHLRVNYPEIGLVCLRTTGNLQDVLALDKFLVENRDHWLV
                     REIILNMARVLRRLDLTAKSFFALVEPGSCFAGNLLELLLAADRSYMLNSDDETVELA
                     ASELNGGALPMGNGLTRLQSRFLAEPERAAAVLEHDGAFDAELAEDAGLITFAPDDLD
                     WEEEVRVAIEERTSLSPDALTGMEASLRFAGPESLDTKIFGRLTAWQNWIFQRPNAVG
                     PEGALTNYGKPTQAKFDYKRT"
     gene            1509..>2455
                     /gene="boxB"
                     /locus_tag="VF240_20210"
     CDS             1509..>2455
                     /gene="boxB"
                     /locus_tag="VF240_20210"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX7177594.1"
                     /translation="MELYERIPNNVNLSEDKKLQRALEKWLPDYLAWWRDMGPEGFQQ
                     KDVFLRTAVDVDAEGWANFDYVKMPDYRWGIFLKPASADARVNFGDNLGAPAYQEVPG
                     EFRNALRRIIVTQGDTEPASVEQQRRLGHTAPSLYDMRNLFQVNVEEGRHLWAMVYLL
                     HKYFGRDGREEADALLERRSGNPDTPRILEAFNQPCDDWLNFYCFTMFTDRDGKYQLS
                     ALAESGFEPLARTCQFMLTEEAHHLFVGETGVGRIVKRTSQLMKESPNEDVRTRGGIP
                     LDMIQRHINFWMSYSLDLFGGEISSNAADFFAAGLKGRF"
BASE COUNT          451 a          799 c          768 g          437 t
ORIGIN      
        1 gtcaccagcg gcaacgaacg ggtgttctgc gccggcgcca acatcccgat gctggccacg
       61 tccacgcacg ccttcaaggt gaacttctgc aagttcacca acgagacccg ctgcgccatc
      121 gaggacgcct cgcgcaactc caatcagcga tacctcgccg ccctcaacgg cacggcctcc
      181 ggcggcggct acgagctggc catcgcctgc gacgaaatct acctcgtgga cgacggcaac
      241 tccgccgtct cgctccccga agtgccgctg ctcggcgtgt tgcccggcac gggcggactg
      301 acgcgcctcg tcgataagcg caaggtgcga cgcgaccgcg ccgacgtgtt ctccacgctc
      361 gccgagggac tgaaggcgaa gcgtgcgacg gagtgggggc tgatcgacgg ctatttcgcg
      421 acgagcaaat ttcaggaggg cgtggaggcg cgcgtggggg agatcgtcgc ggccggcgac
      481 gcttccctcc ccggccccgg catcaagctg aatccgcttg aggtggagac ttccgagaca
      541 ggccgcgagt acaagtacgt cagcgtgcgt ctcaatcgcg aggggcgcta tgcggatttg
      601 accatgcgcg cgcccgtcgg cggcctcccc gcttcggccg atgaggccgt ctcgctcggc
      661 gattcgttct gggcgttgcg cgcctaccgt gagcttgacg acgcgctgct gcacctgcgc
      721 gtcaattatc ccgagatcgg tctggtctgt ctgcggacga ccgggaacct tcaggacgtg
      781 ctcgcgctag acaagtttct cgtcgagaac agagaccact ggttggtgcg cgaaatcatc
      841 ctaaacatgg cgcgcgtgct gcggcgtttg gacctgacgg ccaagagctt cttcgcgctc
      901 gtcgagccgg gtagttgctt cgccggcaac ctgctcgaat tgttgttggc ggcggatcgc
      961 tcctacatgc tcaactcgga cgacgagacg gtggaattgg ccgcgagcga gttgaacggc
     1021 ggcgcgctcc cgatgggcaa cggtttgacg cgcctccagt cgcgctttct ggcggagccg
     1081 gagcgcgcgg ccgctgtgct cgaacacgac ggcgcgttcg acgccgagct tgccgaagac
     1141 gccgggctga tcacttttgc gcccgacgat cttgattggg aggaggaggt gcgcgtcgcc
     1201 atcgaagagc gcacgtcgct ctctcccgac gcgctcacgg ggatggaagc ctctttacga
     1261 ttcgccggcc cggagtcgct cgacacgaag attttcggac ggctcaccgc ctggcagaac
     1321 tggattttcc agcgccccaa cgccgtcggc cccgagggcg cgctgaccaa ctacggcaag
     1381 ccgacgcagg cgaagtttga ttacaagagg acttaaagcc gtgatgagtg atgagtgata
     1441 agtgataaga aaaacagatt tctttcttat cacttatcac tcatcactta tcacggctcg
     1501 gaggaaagat ggagttatac gaacgcattc ctaacaacgt gaacctctcg gaggataaga
     1561 agctccagag ggctttggag aagtggctgc ccgactatct cgcgtggtgg cgcgacatgg
     1621 ggccggaagg ctttcagcaa aaggacgtgt tcttgcgtac cgccgtcgat gtggacgccg
     1681 aagggtgggc caacttcgac tacgtgaaga tgcccgacta ccgctggggc attttcctca
     1741 agccggccag cgcggacgcg cgcgtcaatt tcggcgataa cctcggcgct cccgcctatc
     1801 aggaggtgcc cggcgagttt cgcaacgccc tgcgccgcat catcgtcacg cagggcgaca
     1861 ccgagccggc ctccgtcgag cagcagcgcc ggctgggcca caccgccccg tcgctctacg
     1921 acatgcgaaa tctttttcag gtcaacgtcg aggaaggccg gcacctctgg gcgatggtct
     1981 acctgctcca caagtacttc ggccgcgacg ggcgcgagga ggccgacgcg ttactcgaac
     2041 gccgcagcgg caaccccgac acgccccgca tcctcgaagc cttcaaccag ccctgcgacg
     2101 actggctcaa cttctactgc ttcacgatgt tcaccgaccg cgacggcaag taccaactct
     2161 cggccctcgc cgagtcgggc ttcgagccgc tggcgcgcac ctgccagttc atgttgacgg
     2221 aagaggcgca ccacctcttc gtcggcgaga cgggggtcgg ccggatcgtc aagcgcacgt
     2281 cgcagttgat gaaggagtcg ccgaacgagg acgtgcgcac gcgcggcggc atcccgctcg
     2341 acatgatcca gcgccacatc aacttctgga tgtcttactc gctcgacctc ttcggcggcg
     2401 agatcagctc caacgcggcc gacttcttcg ccgccggcct caagggccgc tttca
//
LOCUS       DASVES010000484         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1120527, whole genome shotgun sequence.
ACCESSION   DASVES010000484 DASVES010000000
VERSION     DASVES010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120527"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1407
                     /locus_tag="VF240_20215"
     CDS             334..1407
                     /locus_tag="VF240_20215"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177595.1"
                     /translation="MDIRFDNVTKRFGASIVVDGLTLSIDDGEFVVLLGPSGCGKTTT
                     LRMLAGLEDATSGDILIGGERMNDVPARARDVAMVFQSYALYPHMTVAENIAYPLRVR
                     KTPRAETQARVSRVASMLEIEPLLARRPRDLSGGERQRVALARAVVREPRAFLMDEPL
                     SNLDAKLRVQTRGELKRLQHELRTTTVYVTHDQTEAMTLAGRVAVMREGRLQQFDTPL
                     AVYHRPANRFVAGFVGNPPMNFVEGRLDAAARLFVAGDLRIDVPERLLLAAGMGRERV
                     VLGVRPEHVRVGGAARAGWLPTTVYVTEQMGNETLVFLRLGAGKIIARAEADFRADPE
                     TPAWITFDDDRLHLFDADSGDAL"
     gene            1592..2413
                     /locus_tag="VF240_20220"
     CDS             1592..2413
                     /locus_tag="VF240_20220"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX7177596.1"
                     /translation="MKLALNGATTMRADLLTDIEAARAAGFDCLEIWAAKLRKFLGNG
                     GTTTELKEAFDAAGIAPYSINSIEHVTFRDAGAHLRILDECEELCRVAAEIGCPYVVV
                     VPGLLPEGVGREGVIDESVRVLGEMARVAEAYGTGLAFEFLGQPRCSVQTLELAAEIV
                     SRLGRADVGLVIDSFHFHAGGSTIESIDALDAERLFIFHINDAEDLPADRLEDRHRLL
                     PGLGILPLGAIIAALRRIGYDRVTSVEIFRPEYWERDPTELAREAKAAVERAISY"
     gene            2524..3510
                     /locus_tag="VF240_20225"
     CDS             2524..3510
                     /locus_tag="VF240_20225"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7177597.1"
                     /translation="MEKIKVGIVGAGYIGGVHASVLARDERVRLTAVHDSTLERAEQL
                     SRATGAEIARDAEELIERVDAVYVTTPNTRHTELAASAAGAGRHVFCEKPLATSLADA
                     RAVLAAARSASGVFQVGHNRRFAPVYARLRRMIREEGAAPHSAHVKMNRGELVSPEWV
                     GDPAVTGGFLYETTIHMFDMLRFLFGEVARLDAVGSAHDVYPETDDFSLLLRFESGVH
                     ASMCSSADASWLFPFERVEVFCHHRTIVTREMESLTDSSGLEGRHTTHSVHQLSKEEK
                     WGYAQEDRAFIDSILSGAPASVTAEDGYKAVELVDACYSAVRSGEPWLGARN"
BASE COUNT          602 a         1168 c         1171 g          628 t
ORIGIN      
        1 catcgccggc cgggtgacgc agtatgagat caggtggggc gtgatgagcg ccgccggggt
       61 agtggcgatg acgccgattc tcatcttcgc gctcgccgtc cagcgtcacc tcgtgcgcgg
      121 attgtcgctc ggagcggtga agggatgaga tgcaactcgg acgctgtttg cccctttgat
      181 gaactcatgt gttgagtatg tgtaacgtgg attcaccaca gaggcgcaga gggcgcagag
      241 ttcacgcaga gggtcgaata tctctgcgcg tcctctgcgc ctctgcggtg agcctcaatg
      301 ctccgaaagc tcaagtcaga gcgaacaaaa cacgtggaca tcaggttcga taacgtcacc
      361 aaaagattcg gcgcgtccat cgtcgttgat gggctgacgc tctccatcga cgacggcgag
      421 ttcgtcgtcc tgctcggccc ttcgggctgc gggaagacga cgacgctcag gatgctcgcc
      481 gggcttgaag acgccacctc gggcgacatc ctgatcggcg gcgagcgcat gaacgacgtg
      541 cccgcgcgcg cgcgcgacgt ggcgatggtc ttccagtctt acgccctcta cccgcacatg
      601 accgtggcgg agaacatcgc ctacccgctg cgcgtcagaa agacgccgcg cgccgagacg
      661 caagcacgcg tgtcgcgcgt cgcctcgatg ctggagatag agcctctgct cgcacgccgg
      721 ccgcgcgacc tctccggcgg tgagcggcaa cgcgtggcct tagcccgcgc cgtggtgcgc
      781 gagccgcggg cgtttctgat ggacgagccg ctgtcgaacc tcgacgcgaa gctgcgcgtg
      841 cagacgcgcg gcgagttgaa gcgtctccag cacgagctac ggacgacgac cgtctacgtc
      901 acacacgatc agacggaggc gatgacgctc gccggccgcg tcgccgtgat gcgggagggg
      961 cgcctccagc agttcgacac gcccctcgcc gtctaccacc gccccgccaa ccgcttcgtc
     1021 gccggcttcg tcggcaaccc cccgatgaac ttcgtcgaag gtcgcctcga cgccgccgcg
     1081 cgtctgttcg tcgcgggcga tttgcgaatc gacgtgccgg agcgtctgct gctcgcggcg
     1141 ggcatgggac gcgagcgcgt cgtcctcggc gtccgccccg aacacgtccg cgtcggcggg
     1201 gccgcgcgcg cgggctggct gccgacaacc gtctacgtca ccgagcagat gggcaacgag
     1261 accctcgtct tcctccgcct cggcgccggc aagatcatcg cccgcgccga ggccgacttc
     1321 cgcgccgacc cggagacgcc tgcctggata acattcgacg acgaccggct gcacctcttc
     1381 gacgccgact ccggcgacgc gctctagatg tgccgcccga gcgcggtagc cacgtgcccg
     1441 cgaattctgt tattaaagct gttagctatt agctgttagc tgttagcttg aagagagcga
     1501 acaaaagatt tattcgccgg gggcgtatcg tcttcggctc ttgtttggct gattgctaac
     1561 agctaacagc ttcctctagc agctacttcc tatgaaactc gcactcaacg gcgcgacgac
     1621 gatgcgcgca gaccttctga ccgacatcga ggccgcgcgc gcggccggct ttgactgtct
     1681 ggaaatctgg gccgcgaagc tccgcaagtt tctgggcaat ggcgggacga ccacggagct
     1741 gaaagaggcg ttcgacgcgg ccggcattgc gccctacagc atcaactcca tcgagcacgt
     1801 caccttcaga gacgcgggcg cacaccttcg catactcgac gagtgtgagg aactgtgccg
     1861 cgtggccgcc gagatcggtt gcccttacgt cgtcgtggtg ccgggtctgc tgcccgaggg
     1921 cgtggggcgc gagggcgtca tcgatgagtc cgtgcgcgtg ttgggcgaga tggcgcgcgt
     1981 ggccgaagcc tacggcacgg ggctggcgtt cgagtttctg ggccagccgc gttgcagcgt
     2041 gcagacgctg gagctggccg ccgagatcgt ctcgcgcttg gggcgcgccg acgtggggct
     2101 ggtcatcgac agcttccact tccacgcggg cggctcgacc atcgagtcca tcgatgcgct
     2161 cgacgccgag cggcttttca tctttcacat caacgacgcc gaagacctcc ccgccgaccg
     2221 tctcgaagac cggcaccggc tgctgccggg cctcggcatc ctccccttag gcgcaatcat
     2281 cgccgcgctc cggcgcatcg gctacgaccg cgtcacgagc gtcgagattt tccgacccga
     2341 gtactgggag cgcgacccca cggagcttgc gcgcgaagct aaagcggcgg tggaaagagc
     2401 tattagctat tagctgttag ctgttagctc acggcttccg ccgtcgtggg cgttgccttc
     2461 gtgaagcgaa cttcgttggt cgctatcttt aagctaatag ctaacagcta atagctaatg
     2521 gctatggaaa aaatcaaagt aggaatcgtc ggggccggct acatcggcgg cgttcacgct
     2581 tcggtgctgg cgcgcgacga gcgcgtgcgt ctgacggcgg tgcatgactc tacacttgag
     2641 cgtgccgagc agctttcgcg tgcgacgggc gcggagattg cgcgcgacgc ggaggagttg
     2701 attgaacgtg tggacgccgt ctacgtcacg acgcccaaca cgaggcacac ggagctggcc
     2761 gcgtcggccg ccggcgcggg ccggcacgtc ttctgcgaaa agccgctggc gacctcgctc
     2821 gcggacgcgc gcgccgtgct cgcggcggcg cgcagcgcga gcggcgtctt tcaggtcgga
     2881 cacaaccgcc gcttcgcgcc cgtctacgcc cggctccggc ggatgattcg cgaggaaggg
     2941 gccgcgccgc actcggctca cgtcaagatg aatcgcggcg agttggtctc gcccgaatgg
     3001 gtgggcgacc ccgctgtgac cggcggcttc ctctacgaga cgaccatcca catgttcgac
     3061 atgctgcgct tcctcttcgg cgaggtcgcg cgtctcgacg ccgtcggctc cgcgcacgac
     3121 gtgtacccgg agacggacga cttctccctg ttgctccgtt tcgagagcgg agtacacgcg
     3181 tcgatgtgct cgtcggccga cgcgagctgg ctgtttccgt tcgagcgcgt cgaggtcttc
     3241 tgccaccacc gcaccatcgt cacgcgcgag atggagagcc tcaccgacag cagcggactc
     3301 gaaggccgac acacgaccca ctccgtccac cagctctcga aggaagaaaa gtggggctac
     3361 gcgcaggagg atcgcgcctt catcgactcg attctttcgg gcgcgcccgc ctccgtcacg
     3421 gccgaggacg gctacaaggc ggtcgaactg gttgacgcct gttacagcgc cgtccgcagc
     3481 ggcgagccgt ggctcggtgc gagaaactag agtgggggca gaagcccgac cgtcagggag
     3541 ggcgtctttg acactggctg aagacgccc
//
LOCUS       DASVES010000485        20305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1457571, whole genome shotgun sequence.
ACCESSION   DASVES010000485 DASVES010000000
VERSION     DASVES010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20305
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457571"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VF240_20230"
     CDS             <1..623
                     /locus_tag="VF240_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177598.1"
                     /translation="GVAGWVVDKSNPAAHVEVQLYVNGRFVESRQADFGRADLVAAGV
                     AEDARHGFFFYLPPLEEGRHEARVYAVHESGSGTRRTLRQLGEAIEFALDATPAEPLY
                     RGWLDEATPVSVRGWVASRADGEARVEVELHIDGRASGRRFADEPRPDLAAAGVPGDG
                     RHGFFFFTPPLLPGEHEARVYVVHAAGEGAAPTLRLVGRPIRFNVP"
     gene            647..1351
                     /locus_tag="VF240_20235"
     CDS             647..1351
                     /locus_tag="VF240_20235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7177599.1"
                     /translation="MSVDADLTRPADADDPVATGEAGERLLALWEIASVTLSFLAAAW
                     VAAPFAENNSKLVGAVPVVFALVLIWLSHRARGETARAVGWRADNFGRALRLLALPML
                     GAAVVVVLVGWTYRSFRVSKLENWQWVLWLFAWGLIQQYALHGFVNRRAQLVWGRGWR
                     SVLAVAAVFALLHLPNPWLALATFAGGVAWAAVYQRAPNIPALAVSHALMSLLLVWAL
                     PPWVMKSLRVGFKYFN"
     gene            1699..2619
                     /locus_tag="VF240_20240"
     CDS             1699..2619
                     /locus_tag="VF240_20240"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177600.1"
                     /translation="MSGEQAGPTEALNYESGDGDDGGAPPRRAAPHVLPLAPVVANEA
                     GAALRLRDLGDVWAFRELLYFLTWRDIRVRYKQTAMGAAWAIIQPLVMMLVFAVVFGM
                     MGVPTDGMPYFIFFYCGLLPWTFFSGAVTNSSLSLVTNGNLISKVYFPRVLIPTATIG
                     AGLVDLAVASVILVLLAFYYGMPATRNLLMLPVLVVLTVLMSLGLGMWLAALTVKYRD
                     VRHVLPFTLQVWFFVTPIIYPANLLDVKWRWLLYLNPMTGIVEGLRSAISGRAFDWPS
                     IAFSATVTLAVLVISIFAFNRIEKSFADLI"
     gene            2630..3961
                     /locus_tag="VF240_20245"
     CDS             2630..3961
                     /locus_tag="VF240_20245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177601.1"
                     /translation="MKPIIKVENLSKSYRIGELEAGYATLREAVARKVAASLRRRMRR
                     GEAGETLWALKDLDFTVQPGEVLGLIGHNGAGKSTLLKVLSRITVPTTGRTEVYGRIG
                     TLLEVGTGFHPDLTGRENIYLNGAILGIRRAEIARKLDEIIAFAELEKFIDTPVKWYS
                     TGMYLRLAFSVASNLDTEVLFMDEVLAVGDVGFQQKCLDKMHEIRQQGRTILFVSHSM
                     AAVTRLCQRVIWLDHGRMVQDGPAHAVVNDYLGTAWKVTAERVWGEEEEAPGDHVVRL
                     RRVRARERGGATAESLDIRHPVGIELTYDVLVEGEVLTPKVELLNEEGAALFSAHDVG
                     ERWRYRPRPVGRYVSTVWIPGNYLAEGNLRAHASIVSHTPATVLHAPAPNAVGFQVVD
                     SQHKDSARGDYVGPIPGLVRPLLDWTTEFDAGDAAVPETVPDTTESLEVPT"
     gene            3958..6054
                     /locus_tag="VF240_20250"
     CDS             3958..6054
                     /locus_tag="VF240_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177602.1"
                     /translation="MRSAVFEKAKHHLPALGALSLLHWAVFARLIFLSPERVPYATVP
                     FDFAAAYAPWLIFIGDSFRAGIFPLWSPYVSAGTPFFINPQSQLYSPLTLLIASTIGY
                     TQRVAQLQTVLMLLAGGVGAYFLAHAIWRWRAAALVTAVCFTFSSAVFPNLEHMTITN
                     ACALTPWAFWAATMAGREARPWAYPVLAFLVYFLITSGYPAVVLMTLLWLLAYTLYLI
                     HLRGGTRRERLRHTLSHAFAWALGLGLAGAHWIPIAAGRREFTRGAPLPLDQALLGGH
                     VYFKYLWGTVFQFLATHSLAGDNSDISMRGLYVGAVALPLAGAALLLSKEHITRPLLL
                     LTVGAFLMACGGMFFGRVFLHILVPALNVSRFPSADSRALMALGLALLAGGGAALLAD
                     GHPRARRVVARGCVLLLGVFTLGLFAFRAVLDAAVYDNIAVNYITAEFLFVGLALLAL
                     RSSTRRALMLTLVAALALEVGTSVLTNMKMVGDPVEEAGSYRALWASRRAPFDPRLAD
                     ATRRAGGTDLVSEESGQGYVQKIFYLTDYNPLRLRRFDSLISAGFTDWLTNGKRVVAL
                     PPDSRPGGYDSFQQLARPASYTILEFTPNRVVYQVNAEADTLLVFNEVYFPGWRAEVE
                     GRAQGMIDFGGLRALRVAAGEHVINTSFRPGTFYWGLGVSLASLLVFLTWLILLARRH
                     RRARARARLETAGVAV"
     gene            6067..7059
                     /locus_tag="VF240_20255"
     CDS             6067..7059
                     /locus_tag="VF240_20255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7177603.1"
                     /translation="MLLSVIVPCYNEEAVLRETHRRLREALAALEGARFELIYVNDGS
                     RDKTLEMLHELQTECEEVRVVSLSRNFGHQVAVTAGVEHAAGEAVVVIDADLQDPPEV
                     IGEMVALWREGYHVVYGVRAERAGESSFKLWTAKAFYRLLNRLSEVKIPLDAGDFRLM
                     DRQVIDAMTAMPERDRYLRGMVSWVGFRQIAVLYQRAPRHAGESKYPLLKMFNFALDG
                     VISFSSLPLRLAVWTGFLIIGLILAAIVVAVLLRLYTHDWVRGWASLFVAVLFMGAVQ
                     LVFLGIIGEYVGRIYRETKRRPLYFVEERLGFGTRADDEGQPATEQRRAQSSRP"
     gene            complement(7172..7942)
                     /locus_tag="VF240_20260"
     CDS             complement(7172..7942)
                     /locus_tag="VF240_20260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7177604.1"
                     /translation="METKLNRESRLSGDDTNPGREVDLLSGKVDFDGYADEYDAHLGQ
                     AISVSGEDKDYFARGRIDWLSKYLKRELIGVESVLDFGCGTGGSTPHLLELLGAGLVL
                     GLDVSSKSLDVARRLHGEDRARFMLFGEYRPAQEVDLAVANNVFHHIAPAERRDSVQY
                     IFDSLRPGGLLAFWENNPWNPATRYVMSRCEFDQDAVTLTFPEARGLLRGGGFEVIRT
                     DFLFIFPRALRHLRWIEPHVARLPLGTQYLVLCRKPQN"
     gene            8195..11803
                     /locus_tag="VF240_20265"
     CDS             8195..11803
                     /locus_tag="VF240_20265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177605.1"
                     /translation="MMKFNPLDHPICFSLPLRIAPSAWMGHVPFAMYLIDLLRPKAVA
                     ELGTHYGVSYCAFCQALKELGVNSRCYAIDTWGGDPQTGFYGPEVLTDLKTHHDPLYG
                     SFSHLIQSTFDEALNYFPDQTFDLLHIDGFHTYESVKSDFEKWLPKMTERGVVLFHDI
                     NVRERDFGVWKLWGELKTQYPHFEFVHSYGLGVLAVGRDYPGELQEFFNCSEKGASIA
                     RHFFSQLGARLEAVKELQTLRQSGEQAASAAQREKQGLEAQLRERDTQIHEKNLQLTS
                     LAQQLQERDSQINEKSLRLAEHTQRLEGESESQLRERDTQIHERNLQIATLIQQVRGR
                     DEQIHEKNLALAELSRQVGEASRQAGERDTQIHEKNLQLTSLAQQLQERDTQINEKNL
                     QLQAVRLQVEEKDRQLQQSLQQLEEMNRQADEKVALLGEKDQQLKAIDQRLQRHLVQL
                     HEAKRQRQANEQALQESGQQIQQLSLQLQQQGRKLRSINEQLQLKERQLQFKIQQLQA
                     REQSEQEKCQQLRDKDLISSQAQHRISEVERQLQERLLELEGKERTIQAYARVVEDFG
                     NSGSYRLGRAISWPLRMLKQQLDDAPNGSQPHSPVQQIQLPPDETIEAPRPPAQPQHL
                     PDGNPAYDYQLWVELFDTLTDSDREAILSRVNRLEHKPLFSIVMPVYNVEEVWLRKAI
                     ESVRRQLYPLWELCIADDNSTQPHVRKVLDEYVRKDPRIKVTYRQTNGHISAASNSAL
                     ELASGEFIVLLDNDDELPEHALYMVAEEINDHPEADLIYSDEDKIDETGRRREPHFKS
                     DWNPDLLYSCNTISHLGVYRASIIKQIRGFREGYEGSQDYDLALRFIEQIPESHIRHV
                     PHVLYHWRAIAGSVALASGEKNYAHEAARRAIRSHFQRRGIEAAVTEGHNCFHRVSYP
                     VPDPAPLVSLIIATRNRLDLLEQTIHGLLNETDYGPIEIIIIDNQSTDTAALRYLRQI
                     QADPRVRVIAYDAPFNYSAINNLGVRESGGEVIGLINNDIKVISPGWLREMVSHAVRP
                     DVGAVGAKLLYPDDTVQHAGVILGVHSVAAHAHRHIPKDSPGYLNRAMSIQNLSAVTG
                     ACMVLRREVFDEVGGLDEVNLPVAFNDVDLCIRIRESGYRIVWTPYAQLYHLESASRG
                     RDDTAENAPRFSKEIQYMMYVWKDALAGDPYYNPNLTLVGEDFSLAVPPRAAKRWR"
     gene            12213..12725
                     /locus_tag="VF240_20270"
     CDS             12213..12725
                     /locus_tag="VF240_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177606.1"
                     /translation="MLTIAGFSACTGDADDVPAANNVASNGASQPSSTSEIDIKSLVS
                     VNSDTSLAMDGISGQPAKPGIPVVINPKSTPSFAITGWAVDQQAQTEAGGVIINIDGS
                     KDVLAKYGQPRPDVAAHLKNTKYTNSGFIATMDASSLAKGRHTLTVRVVTADRKGYFE
                     HKNKYEIEIQ"
     gene            12836..14890
                     /locus_tag="VF240_20275"
     CDS             12836..14890
                     /locus_tag="VF240_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177607.1"
                     /translation="MFIVACVAIALFKLWLVRGEEVGYSGSYFDDVWYVQSAKDWYWL
                     RSYRELSFDTQPYIRLPAYPLYIALANLTGFPLRIVNELLFLLVAFAFSWVLVKAGLR
                     RFLCLVLYVAIVLHPGSFAVNSVLSPDPFFASVLLGALAGMILLFVKRGETNRVWYGS
                     VAGLALAVLWHARQENIIVLGLLVVYVLLLLLVLRERGSPWASTLKELSVVAGVPVAV
                     VLMASLAVKTINYQRFGVFAADAMFTSDFERATKALLRIKPAVPLRYVPVPREVRERA
                     YEVSPAFRELRPFFEGNVGRTWASFGKDAGVQAEGEISAGHVWWALNQGTYQIGYNKS
                     ARQAGRFYKRIADEVNAACDDGRLACRTVFSSLIDPHVQNYWPHLPGSLVRMTSLFTS
                     TAEVPKPRDANHLPPDLRVLFDSVTNRRTALQNPPAMRIRGWAFNPGDKLQGVLVRNQ
                     DGRVLAGGRLTPRHDVAAAFTSQNGTAPLDTGFDLLLPPSSPMPYNADLVFTTGSGKE
                     LIVRRPGGAADSPGPGLTYHVDEDMTFPDSHRLELATQSYIWSVHGKIIMLLTYAGLC
                     ALLLALIFFRSVKLREPAVFVSVLLAAAIVSRLALFTLIDAAAYIAAEIRYIFPVTYL
                     YTCLLLILIHYSLSLVTARLDGSSVLQRIDIRKLLKKTMEKSARWKQEKPRTGESRQQ
                     VG"
     gene            complement(15064..15237)
                     /locus_tag="VF240_20280"
     CDS             complement(15064..15237)
                     /locus_tag="VF240_20280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX7177608.1"
                     /translation="MTNLYPKLSAGGFIIIDDYKAVPACKPAVDDYRRVHGITEEIVE
                     IDWAGVYWRRLRP"
     gene            complement(15451..16479)
                     /locus_tag="VF240_20285"
     CDS             complement(15451..16479)
                     /locus_tag="VF240_20285"
                     /inference="COORDINATES: protein motif:HMM:NF015350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HEX7177609.1"
                     /translation="MKNSWIVLTTINPPTKAVESIARLCDRGWSAVVVGDTKTPPRWH
                     CDGVTYLDVAAQRTMFGTFADAIPYKHYSRKNLGYLHAIRQGAELILETDDDNIPYDT
                     FGQELSRSVSGRLVDGPGWVNIYKHFTDDAHIWPRGLPLDAIDNRGRVTPLATSSPCP
                     IQQYLADNDPDVDAVYRLTNKRPVFFEREARPVVLAKDVWVPFNSQNTVFFREAFPLL
                     YLPCFVSFRMTDIWRSFVAQAALAHHGLSVSFHPPTVEQLRNEHDLMKDFSDEVVGYL
                     RNREIGEILTHTLSGLEPNTSDLSTTALTLWRALSDAEVIPPTEIPIIEEWFSRVADS
                     EVLADEAR"
     gene            complement(16445..17233)
                     /locus_tag="VF240_20290"
     CDS             complement(16445..17233)
                     /locus_tag="VF240_20290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177610.1"
                     /translation="MTEIDDKTVSLILVVRNEEVGLRQVLPKISTDIFDDFFAIDGTS
                     TDKSVELMKSFGYQAYPQVKRGLGSAMIEARERVKTHSFIYFHPDGNEDPADLPRMAE
                     LLRAGHEFVVASRMIQGAWNEEDDKVFKWRKYANQGFALLANVFFAHGGNRTSDVTHG
                     FRGITCETFDRMNLSSRDLTMDYQMVIRALKLGIRITEFPTHEWPRIDGATNFASLPT
                     GIAELKLLWREIAMGLRSAEGKELRIASKESLRREKFLDSTDHN"
     gene            complement(17362..20145)
                     /locus_tag="VF240_20295"
     CDS             complement(17362..20145)
                     /locus_tag="VF240_20295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177611.1"
                     /translation="MKPIQAYDESPSGGLPGGAPPESNGLALPPPASAAPPEEAHGAL
                     PEAWPECAAGSRAAADSVESRPRMHQAQVLTELRARVKAQAEALRWITLSRSWRYTSW
                     LRRLNFLLLRLRTRLGSLQPASFHGALEQPAEVDEISQEIEIRGWAYSEGAAVVRVEA
                     FLDTISLGPLCYGKTRLDVAPYPSRAPVKCGYEGRLTVDESLFVGRRRLTVRVTDQRG
                     RIEDYEQMVEVPGPAERPAGPGAEPAPGDGTWARTDAFSLLAQDSLSFSKKTLESMCK
                     FSLGIFFSSDSVIEFPQHETPATSIILVLYNRAELTLKCLQSILNNHKDPYEVVIIDN
                     ASTDETPALLGRVRGARVVRNETNVHYLRACNQAARLARGRNLLLLNNDAQLLNDAVA
                     AATETLDSSDDIGAVGGRIILPDVTLQEAGSIVWQDGSCHGYGRGGAPNAPEFLFRRD
                     VDYCSAVFLLTRRELFLEGGGFDEAFAPAYYEDADYCVRLWKRGKRVVYDPRVSVLHY
                     EFGSAVSFRRALEMQSRHQPVFAAQHREWLSAQQPFSPSNVLAARTHALPRCRRVLYL
                     DDRVPHAHLGSGFPRSNRIVSEMVAMGHCVTCYPLDQPHEEWAEVYGDIPREVEVMLG
                     RGRAGLEEFLAERAGYYDLVYVSRPHNMAALKALLERHPQLLAGAKVVYDAEALFSLR
                     EAERLRLRGRAPSEREKEGQVSEEVGLAVGCDAVVSVSDGEGREFARRGFSNVHTLGH
                     TVNARPTPRGFDERRDILFVGAIHDPDSPNADSVAWFGKSVLPLIQKSLGEDVRLLVA
                     GHASRDFFAGLDNGHVKVLGRVEDLTELYDRARLFVAPTRFSAGLPYKVHEAAAYGLP
                     VVATTLIGGQLGWAHEEEVLLADDAKGFASACIRLYRDERLWTLLRENALKRVEMDCS
                     PVSFSERLTKILR"
BASE COUNT         3766 a         6295 c         6434 g         3810 t
ORIGIN      
        1 gaggggtcgc cggatgggtg gtcgataagt cgaaccccgc ggcgcacgtc gaggtgcagc
       61 tctacgtgaa cggccgcttt gtcgagagcc ggcaggccga cttcgggcgg gccgacctcg
      121 tggcggcggg tgtggctgaa gatgcgcggc acggattctt cttctatttg ccgccgctcg
      181 aagagggccg gcacgaggcg cgcgtttacg ccgtacacga aagcggcagt ggtacgcgtc
      241 ggacactccg acaactcggg gaggccattg agttcgcgct cgacgcgacc ccggccgagc
      301 cgctctatcg cggctggctc gacgaagcga cgcccgtctc cgtgcgcggg tgggtcgcga
      361 gccgcgcgga cggcgaggcg cgcgtcgagg tcgagctgca catcgacggg cgcgcgtcgg
      421 gccggcgctt cgccgacgaa cctcggcccg acctcgccgc agcgggcgtg cccggcgacg
      481 gccgccacgg cttcttcttc ttcacgccgc cgcttttgcc cggagagcac gaggcgcgcg
      541 tctacgtcgt ccacgcggcg ggcgagggcg cggcgccgac gctgcggctc gtcgggcggc
      601 ccattcgttt caacgttccg tgaggcatga gtcgttcgag tgagacatga gtgtggacgc
      661 tgatttgact cggcccgccg acgcggacga ccccgtggcg acgggcgaag cgggcgagcg
      721 attgctggcg ctgtgggaga tcgcgtcggt gacgctgtcg ttcctcgcgg cggcgtgggt
      781 ggcggcgccg tttgcggaga acaacagcaa gctggtcggg gccgtgcccg tggtctttgc
      841 gctcgtgctg atatggctgt cgcaccgcgc gcgcggcgag acggcgcgcg ccgtgggctg
      901 gcgcgcggac aacttcgggc gcgcgctgcg gttgctcgcg ttgccgatgc tcggggcggc
      961 cgtcgtcgtc gtcctcgtcg gctggacgta ccggagcttt cgggtgagca agctggagaa
     1021 ctggcagtgg gtgctgtggc tcttcgcgtg ggggctgata cagcagtacg cgctccacgg
     1081 gttcgtcaac cggcgcgcgc agttggtctg gggacgtggc tggaggagcg tgctggcggt
     1141 ggccgccgtc ttcgcgttgc tgcacctgcc gaacccgtgg ctcgcgctgg cgacgtttgc
     1201 gggcggcgtc gcgtgggccg ccgtctacca gcgcgcgccg aacatcccgg cgctggccgt
     1261 ctcgcacgcg ctgatgtcgc tgctcctcgt ctgggcgctg ccgccgtggg tgatgaagag
     1321 cctgcgagtc ggcttcaagt atttcaactg aacggggaag tatttcagac gagccgggcc
     1381 ggggtcatgg cctaatattg attctctgcg ccgcctccgc gccctttgcg actccgcggt
     1441 gagtgcggcc ggggagagat tcaccgccga gccgcggagg acgccgagga cgcgcagagt
     1501 acattctcaa ttcaggacac tactcgggcc ggggctgttt aggaaatgcg cggcgggcgt
     1561 gccataatcc cccgcgacgc gcgagcagca gagacttcca cgttccgtgc gaacccccat
     1621 ccacggacga ctgtgaaacc ccgagcacaa atccggcgag agacgttcta gcgaccttcg
     1681 tgtgggcggt atagcgtgat gagcggtgaa caggcaggcc cgaccgaggc tttgaattat
     1741 gagagcgggg acggcgacga cgggggagcg ccgccgcgcc gcgccgcgcc gcacgtcctg
     1801 ccactcgcac ccgtcgtcgc caacgaggcg ggggcggcgc tgcgtctgcg cgacctcggc
     1861 gacgtgtggg cgttccgcga gctgctctat tttctcacct ggcgcgacat cagggtgcgc
     1921 tacaagcaga cggcgatggg cgccgcgtgg gcgatcatcc agccgctcgt gatgatgctc
     1981 gtcttcgcgg tcgtcttcgg gatgatgggc gtcccgaccg acggcatgcc ctacttcatc
     2041 ttcttctact gcgggctgct gccgtggacg tttttctccg gcgccgttac caacagcagc
     2101 ctcagtctcg tgacgaacgg caatctcatc tcgaaggtct atttcccgcg cgtgctgatc
     2161 ccgacggcga ccatcggggc gggcctcgtc gacttggcgg tggcctccgt catcctcgtc
     2221 ctgctcgcct tctactacgg gatgccggcg acgcgcaatc tgttgatgct gcccgtcctg
     2281 gtggtgctca ccgtgctcat gtcgctcggt ttgggcatgt ggctggcggc gctcacggtg
     2341 aagtaccgcg acgtgcggca cgtgctgccg ttcacgctcc aggtctggtt cttcgtcacc
     2401 ccgatcatct acccggcgaa cctcctggac gtgaagtggc gctggctgct ctacctcaac
     2461 ccgatgaccg gcatcgtcga gggcctgcgc tccgccatca gcgggcgggc gttcgactgg
     2521 ccctccatcg ccttctcggc gacggtgacg ctggcggtgc tcgtcatttc gatcttcgcc
     2581 ttcaatcgca tcgagaagag cttcgccgac ctcatctgac agaagacgcg tgaaaccaat
     2641 catcaaagtc gagaacctga gcaagagcta ccgcatcggc gagttggagg cgggctacgc
     2701 cacgctgcgc gaggccgtcg cgcgcaaggt cgccgcctcg ctccggcggc ggatgcggcg
     2761 gggcgaggcc ggcgagacgc tctgggcctt gaaagacctc gacttcaccg tgcagcccgg
     2821 agaggtgttg ggcctcatcg gccacaacgg ggcgggcaag tccacgctcc tcaaagtcct
     2881 ctcgcgcatc accgtgccga cgacggggcg gacggaagtc tacggccgca tcggcaccct
     2941 gctcgaagtc ggcacgggct tccaccccga cctcacgggg cgcgaaaaca tctacctcaa
     3001 cggcgccatc ctcggcatcc gccgcgccga gatcgcgcgc aagctcgacg agatcatcgc
     3061 cttcgccgag ctggaaaagt ttatcgacac ccccgtcaag tggtactcca cggggatgta
     3121 tctgcgcctg gccttctcgg tcgcctcgaa cctcgacacg gaagtcctct tcatggacga
     3181 ggtgctggcg gtgggcgacg tgggcttcca gcagaagtgc ctcgacaaga tgcacgagat
     3241 caggcaacag gggcgcacca tcctcttcgt ctcgcacagc atggcggccg tcacgcgtct
     3301 gtgccagcgc gtcatctggc tcgaccacgg gcggatggtg caggacgggc cggcgcacgc
     3361 cgtcgtcaac gactacctcg gcacggcgtg gaaggtgacg gccgagcgcg tgtgggggga
     3421 agaggaggaa gcgccgggcg accacgtcgt gcgattgcga cgcgtgcgcg cgcgcgagcg
     3481 cggcggcgcg accgccgaga gtttggacat ccgccacccg gtcggcatcg agctgaccta
     3541 cgacgtgctc gtcgagggcg aggtgctgac gccgaaggtc gagctgctca acgaggaggg
     3601 cgcggcgctc ttctcggcgc acgacgtggg cgagcgttgg cgctaccggc cgcgccccgt
     3661 cgggcgctac gtgagcacgg tctggattcc cggcaactac ctcgccgagg gcaacctgcg
     3721 cgcgcacgcc tcaatcgtct cgcacacgcc ggccaccgtc ctccatgcgc ccgcgcccaa
     3781 cgccgtcggc tttcaggtgg ttgactccca gcacaaggac tcggcgcgcg gcgactacgt
     3841 cggccccatc cccggcctcg tcaggccgct gctggattgg acgaccgagt tcgacgcggg
     3901 cgacgcggcc gtgccggaga ccgtccccga caccacggag agcctcgaag ttccgacatg
     3961 agaagcgccg tgttcgagaa ggcgaaacat cacctgccgg cgctcggcgc cctgtcgttg
     4021 ctccactggg ccgtcttcgc gcggctcatc ttcctgtcgc cggagcgcgt gccgtatgcg
     4081 accgtgccgt tcgacttcgc ggcggcctac gcgccctggc tcatcttcat cggcgacagt
     4141 ttccgcgccg gcatcttccc cctctggtcg ccctacgtca gcgccggcac ccccttcttc
     4201 atcaacccgc agagtcagct ctactcgccg ctcacactcc tcatcgcctc caccatcggc
     4261 tacacgcagc gcgtcgcgca gttgcagacc gtcctcatgc tccttgcggg cggcgtcggg
     4321 gcctacttcc tcgcgcacgc catctggcgt tggcgcgcgg ccgccctcgt cacggccgtc
     4381 tgcttcacct tctccagcgc cgtcttcccc aacctcgaac acatgaccat cacgaacgcc
     4441 tgcgcgctga ccccatgggc gttctgggcg gcgacgatgg ccggtcgtga agcgaggccg
     4501 tgggcttacc ccgtgctcgc cttcctcgtc tacttcctca tcacgagcgg ctatccggcc
     4561 gtcgtcctga tgacgctgct gtggctcctc gcctacacgc tctacctcat ccacctgcgc
     4621 ggcggcacgc gccgggagcg cctgcgccac acactctccc acgccttcgc ctgggcgctc
     4681 gggctggggc tggccggagc ccactggatt ccgattgccg cggggcggcg cgaattcacg
     4741 cgcggcgcgc cgctgccgct cgatcaggcg cttttgggcg gacacgtcta cttcaaatac
     4801 ctttggggca cggtctttca gttcctcgca acgcactcgc tcgccggcga caattccgac
     4861 atctcgatgc gcggccttta cgtcggagcc gtcgcgctgc cgctcgccgg ggctgcgctg
     4921 ctgctgtcga aggagcacat cacgcggccg ctgctgctgc tgacggtggg ggccttcctg
     4981 atggcctgcg gcgggatgtt cttcggccgc gtcttcctgc acatcctcgt gccggcgctc
     5041 aacgtgtcgc gcttcccgtc ggccgacagc cgcgctctga tggcgctcgg gctggcgctc
     5101 ctcgccgggg gcggggcggc gctcctcgcc gacggacatc cgcgcgcacg ccgcgtcgtc
     5161 gcgcgcgggt gcgtcctgtt gctcggcgtg ttcacgctcg gactcttcgc cttccgcgcc
     5221 gtcctcgacg cggccgtcta cgacaacatt gccgtcaact acatcactgc cgagtttctt
     5281 ttcgtcgggc tggcgcttct ggcgctgcgc tcatccacgc gccgcgccct gatgctcacg
     5341 ctcgtcgccg cgctggcgct cgaagtgggg acgagcgtgc tcaccaacat gaagatggtg
     5401 ggcgatcccg tcgaggaggc cggcagctac cgcgcgctgt gggcgagccg ccgcgcgccc
     5461 ttcgacccac gcctcgccga cgcgacgcgc cgcgccggcg gcacagacct cgtgagcgaa
     5521 gagtcgggac aagggtacgt gcagaagatt ttttacctga ccgattacaa cccgctccgt
     5581 ctgcgtcgct tcgacagtct catcagcgct ggcttcaccg actggctgac gaacggcaag
     5641 cgcgtcgtgg cgctgccgcc cgacagccgc cccggcggct acgactcgtt ccaacagctc
     5701 gcgcggcccg cctcgtacac cattctcgaa ttcacgccca accgggtcgt ctatcaagtg
     5761 aacgccgagg ccgacacact gctcgtcttc aacgaggtgt attttccggg gtggcgggcc
     5821 gaggtggagg gccgggcgca ggggatgatc gacttcggcg ggctgcgggc gctacgtgtc
     5881 gcggccggcg agcacgtcat caacacttcg ttccggccgg ggactttcta ctggggcctc
     5941 ggcgtgtcgc tcgcctcgct cctcgtcttc ctgacatggc tcatacttct cgcgcgccgc
     6001 catcgtcgcg cgcgggcgcg cgcgcggttg gagaccgcgg gcgtcgcggt ctgaggaatc
     6061 ccacagatgc tactttcggt aatcgtcccc tgctacaacg aggaggccgt gctgcgggag
     6121 acgcaccgcc ggctgcgcga ggcgctcgcc gcgttggagg gcgcgcgctt cgagttgatc
     6181 tacgtcaacg acgggagccg cgacaaaaca ctcgaaatgc tccacgagtt gcagaccgag
     6241 tgtgaggagg tgcgcgtcgt cagtctgtcg cgcaacttcg gccatcaggt cgcggtcacg
     6301 gccggggtgg agcacgccgc gggcgaggcc gtcgtcgtca tcgacgccga cctgcaagac
     6361 ccgcccgagg tcatcggcga gatggtcgcg ctctggcgcg agggctacca cgtcgtctac
     6421 ggcgtgaggg ccgagagggc cggcgagtcg tccttcaagc tctggacggc gaaggctttt
     6481 taccgactgc tcaatcgact ctccgaggtg aaaatcccgc tcgacgcggg cgacttccgc
     6541 ctgatggatc gtcaggtgat cgacgcgatg acggcgatgc ccgagcgcga ccgctacctg
     6601 cgcgggatgg tcagttgggt cggcttccgc cagatcgccg tgctctacca gcgcgcgccg
     6661 cgacacgccg gcgagagcaa atatccgctc ctgaagatgt tcaacttcgc cttagacggc
     6721 gtcatctcct tctcgtcgct tccgctgcgg ctcgccgtct ggacgggctt tctcatcatc
     6781 ggcctcatcc tcgcggccat cgtcgtcgcc gtcctgttgc gactctacac gcacgactgg
     6841 gtgcgcggct gggcctcgct ctttgtcgcc gtcctcttca tgggcgccgt ccagctcgtc
     6901 ttcctcggca tcatcggcga gtacgtcgga cgcatctacc gcgagacgaa gcgccgcccg
     6961 ctctacttcg tcgaggagcg tctgggcttc gggacgcggg ccgacgacga ggggcagccg
     7021 gcgacggagc aacggcgggc gcagagttcg aggccctgac ttcaggatga gtggacgcag
     7081 cagtaccgcg agcggaagcc tcgcgggtgt cgatgcgcga ggcgggcctc tgattgtatg
     7141 cacacccgct cgctcccgct cgcggtactg ttcagttctg gggcttgcgg cacaggacga
     7201 ggtactgcgt gccgagcggc aggcgcgcga cgtgcggctc gatccagcgg aggtggcgaa
     7261 gcgccctggg gaagatgaag aggaagtcgg tgcggataac ttcaaagccc ccgccgcgca
     7321 acaggccgcg cgcctcgggg aaagtgaggg tgacggcgtc ctggtcgaac tcgcaccggc
     7381 tcatcacgta gcgcgtcgcc gggttccacg ggttgttctc ccagaaggcc agcaggccgc
     7441 cgggccggag cgagtcgaaa atgtattgca ccgagtcgcg gcgctcggcg ggcgcgatgt
     7501 ggtggaagac gttgttggcc acggccaggt caacctcctg cgcgggccgg tactcgccga
     7561 acagcatgaa ccgcgcgcgg tcttcgccgt gcaggcggcg cgccacgtcg agcgactttg
     7621 acgacacgtc cagcccgagc acgaggccgg ccccgagcag ttcgaggagg tgcggggtgg
     7681 agccgcccgt gccgcagccg aagtccagca cggattcgac accgatcagc tcccgcttca
     7741 agtacttcga cagccagtcg atgcggccgc gcgcgaaata gtccttgtcc tcgccggaga
     7801 cggaaatcgc ctgccccaga tgagcgtcgt actcatccgc gtacccgtca aaatctacct
     7861 tgcccgagag cagatcgacc tcccgacccg ggttagtatc gtcgccactg agtcgggatt
     7921 cacgatttaa tttcgtttcc atctgccgtg caatatcctg ttgtgaaagc tcgggtaagg
     7981 tcgggtgctg ccgcggctgt cagactcctg gtgcggggtt aatgtaaatt gttttccctt
     8041 caattccaac tccgcgcggt caacccgagt catgcgaggg tttccgcgcc ggaagtgcct
     8101 catgtaaaat gagcgttccc ggaaacgtcc cgtgcacccg gttaattcac gtccgcctca
     8161 aataccgccg gcccaataaa cgggtcgtgc cacaatgatg aaattcaatc cactcgacca
     8221 tccgatctgt ttcagccttc ccctgcgaat agccccgtca gcgtggatgg ggcacgtgcc
     8281 gttcgccatg tatctcatcg atctgttaag gccgaaggcg gtcgctgagt taggcacaca
     8341 ttacggcgtc tcctattgcg cgttctgcca ggcgttgaaa gagctgggag ttaactctcg
     8401 ctgctacgcg attgacacct ggggcgggga tccgcagacc ggcttctacg ggccggaagt
     8461 cctcaccgac ctgaagacgc atcacgaccc gttgtacggc agcttctcac acttaattca
     8521 gagcaccttc gatgaagcct taaactactt tcccgatcaa acattcgacc tcttgcatat
     8581 cgacgggttc cacacctacg agtctgtgaa gagtgatttc gagaagtggc tgcccaagat
     8641 gaccgagcgg ggagtagtct tattccacga catcaatgtc cgggaaagag acttcggggt
     8701 gtggaagctt tggggcgagc tgaagacaca gtatccacac ttcgagttcg ttcactccta
     8761 cggactgggc gtcctggcgg tcggccgaga ctaccccggc gaactgcaag agttctttaa
     8821 ctgctcggaa aaaggggcgt ccatcgcccg ccattttttc tcccagctag gcgcccggct
     8881 ggaggccgtc aaagaattgc aaacgctcag gcagtccggc gagcaagctg cttccgcggc
     8941 tcagcgagag aagcaagggc ttgaagcgca gttgcgcgaa agagacacgc aaattcacga
     9001 gaagaatctg cagctcacgt ctctcgcgca gcaacttcag gaaagagact cgcaaatcaa
     9061 cgagaagagc ctgcgattgg ccgagcacac gcagcggctc gaaggggaga gcgagagtca
     9121 actgcgggag agggacacac agattcacga aaggaatctg caaatcgcaa ccctgattca
     9181 gcaagtgcgc ggacgggacg aacaaatcca cgagaagaat ctggcgttgg ccgagctgtc
     9241 gcggcaagta ggggaagcga gccggcaggc gggcgaaaga gacacgcaaa tccacgagaa
     9301 gaatctgcaa ctcacgtctc tcgcgcagca gcttcaggaa agagacacac aaatcaacga
     9361 gaagaatttg cagctccagg cagtccggct acaggtcgaa gagaaggatc ggcaacttca
     9421 gcaatccctg caacagctcg aagagatgaa tcggcaggcg gacgaaaagg tcgcgctcct
     9481 aggcgagaaa gaccagcagc taaaggcaat cgaccaacgg cttcagcgac atctggttca
     9541 actccatgaa gctaaacggc agcgacaggc aaatgagcag gcccttcaag agagcgggca
     9601 gcagattcaa cagttgagtc tgcagcttca gcaacagggt cggaagctca ggagcatcaa
     9661 tgaacaattg cagctcaaag agcggcagtt gcaattcaaa attcaacagt tgcaggcgcg
     9721 ggagcagagc gagcaggaga agtgccaaca gcttcgggac aaagacctga taagcagtca
     9781 ggcccagcac agaatatcgg aagtggaacg gcagcttcag gaaagattgt tagagttgga
     9841 aggtaaggag cgcaccattc aagcttacgc gagggtggtc gaggatttcg gcaactctgg
     9901 ttcttacagg ctcgggaggg cgatatcgtg gcccttaagg atgctcaagc agcaactcga
     9961 cgacgctccc aacggaagcc agcctcacag ccccgtacag caaattcaac ttccacctga
    10021 tgagaccatc gaggcgcccc ggcctccggc gcaaccgcag cacctccctg atggcaaccc
    10081 cgcatacgat tatcagttgt gggtggaatt gttcgatacc ttgaccgatt cggatcggga
    10141 ggcgattctg tcacgcgtca accggctgga gcacaagccc ctgttctcca tcgtgatgcc
    10201 ggtctacaac gtcgaagagg tgtggttgag gaaggccatc gagtccgtcc gccgacagct
    10261 ctatccgctt tgggaactct gcatcgccga tgacaattcg actcaacctc acgtccgcaa
    10321 ggtgctcgac gaatacgtcc gcaaagaccc gcgcattaaa gtgacgtatc gtcaaacgaa
    10381 cgggcatatt tcggccgcct ccaacagcgc gctggaactc gcctccggcg agttcattgt
    10441 tcttctggac aacgacgacg aactcccgga acacgccttg tacatggtgg cggaggagat
    10501 taatgaccac ccggaggccg acctcatcta cagcgatgag gacaagattg acgaaacggg
    10561 ccggcgccgc gagccacact tcaagtcgga ctggaaccct gatttgctct actcctgcaa
    10621 tacgatctca catctgggag tttaccgagc atccatcatc aaacaaatca ggggcttcag
    10681 agaaggctac gaagggagtc aggattatga cctggctctc aggttcatcg agcagattcc
    10741 cgaaagccat attcgacacg ttcctcatgt cctgtatcac tggcgcgcca tagccggatc
    10801 ggtcgccctc gcctcgggcg agaagaatta tgcccatgag gctgcccgca gggccatccg
    10861 ctcccacttt cagaggcggg ggatcgaggc ggccgtcacg gaaggtcata actgcttcca
    10921 ccgcgtcagc tacccggtgc ccgaccccgc ccctttggtg agcctgatca tcgcgacacg
    10981 taaccgcctg gacttactcg aacagacgat tcatggcctg ctcaacgaga ccgattacgg
    11041 gcctatcgaa ataatcatca tcgacaacca gagcaccgac acggccgctt tgagatacct
    11101 caggcagatt caggctgacc cgcgcgtcag ggtgattgcg tatgacgccc cgttcaatta
    11161 ctcggcgatt aacaatcttg gagttcgcga gtcggggggg gaggtcatcg gtctcattaa
    11221 taatgacatc aaagtcatct cacccggatg gctcagggaa atggtgagcc atgcggtcag
    11281 gccggacgtt ggagccgtcg gggctaaatt actctacccg gatgacacgg tgcagcacgc
    11341 cggggtgatc ctgggagtcc acagtgttgc cgcgcacgcg cacaggcaca tccccaagga
    11401 cagtccgggc tatctcaacc gggcgatgag catccagaat ctttcagccg tcaccggcgc
    11461 ttgtatggtg ctgcgtcgtg aagtctttga cgaggtcggc ggcctggacg aggttaatct
    11521 tcccgtcgcc ttcaatgacg tagatttgtg tatcaggatt cgggaaagtg gttatcgcat
    11581 tgtctggacg ccttacgcgc agttgtatca cctcgaatcg gcatcacgcg ggcgcgacga
    11641 cacggcggag aacgcgccgc gattctctaa agagatacag tatatgatgt acgtctggaa
    11701 agatgccctc gccggagacc cgtactataa cccgaactta accctggtgg gtgaggactt
    11761 ctcgctcgcc gtccccccac gggcggcgaa gagatggcgg taatgtattt gggagtatct
    11821 cattagccgt catgtgccgt cgggccaggg ggtttatcaa cttcgtgggc atctgtcgga
    11881 aagagcgttg acggcggtcg gacaggggac ggttgcaggc cgggacgggt gagggttgtt
    11941 cgtggagaat gaatctcgac tttacccctt tggggacagg cggcgaactt gataaaccac
    12001 atacacatga aatatcatat aactcttcag aagcagtccg tctcctttgg cgtcctacac
    12061 ttggccgacg gtaagtggtc accggcgtgg ccgaatgctc ccgccaaatc gaaggacatt
    12121 taggaatcaa attcactacg gaagaggaac cttggaaaat gagctttaat cgaaagcctc
    12181 ggagggcgtc ttcgctagcg ttaatattcg cgatgctgac tattgccggg tttagcgcat
    12241 gtaccggaga tgctgacgat gtccccgccg ctaacaatgt ggcgagcaac ggggcgagcc
    12301 aaccctcttc cacatccgag atagacatta aatctctggt gagtgttaat agcgatactt
    12361 ccctcgccat ggacgggatc agcggtcaac ccgccaagcc cggtatcccc gtcgtgataa
    12421 atccgaagtc cactccgtcc ttcgccatca ccgggtgggc agttgaccaa caagcacaga
    12481 cggaggccgg gggcgttatt attaacatcg acgggagtaa ggatgtcttg gcgaagtacg
    12541 gccagccccg ccctgacgtg gctgcccatc ttaaaaacac gaagtacacg aactcaggat
    12601 tcatagccac gatggacgcc tcgtccttgg cgaaaggtcg tcacaccttg acggtgcgtg
    12661 tggtgacggc ggacaggaaa ggatacttcg aacataaaaa taagtatgag attgaaattc
    12721 agtagaaggt ggggagggtc ggacagcaat ccgtttcatg gccggaggat tcagccccac
    12781 agttgagaag agcgaggcac gagccgattc cctaccattg ccgcgggcgg gcgcgatgtt
    12841 cattgtcgcc tgcgtggcca tcgcgctttt taagctctgg ctggtgcgcg gcgaagaggt
    12901 tggctactcg ggcagctact ttgacgatgt ctggtatgtt caatcggcga aagactggta
    12961 ctggttaagg tcataccgcg aactgtcctt cgatactcaa ccttacatac gcctgcctgc
    13021 ttaccccctc tacatagcgc tcgccaattt aaccggtttc ccattacgca tcgttaacga
    13081 actcctcttt ctgcttgtcg cctttgcctt cagttgggtt ttggtcaaag ccgggctgcg
    13141 ccggttcctg tgccttgtgc tttatgtggc gatagttctt catccgggga gttttgccgt
    13201 taatagcgtt ttgagtcccg acccgttctt cgcgtccgtc ctgctcggcg ctctggcagg
    13261 catgatactg ctcttcgtga agcggggtga gacgaaccgg gtgtggtatg ggtcggtggc
    13321 aggattagcg ctggccgttc tctggcacgc ccgacaggaa aacataatcg tactggggct
    13381 gttggtcgtt tacgtgttgc tgttgctgtt agtcttgcgt gagcggggga gcccgtgggc
    13441 tagcaccctc aaagagttga gcgtggtggc aggtgtcccc gtcgccgtcg ttctgatggc
    13501 gtcgttggcc gtcaagacga tcaactatca gaggttcggc gtgttcgcgg ctgacgccat
    13561 gttcacgtcc gacttcgaaa gagccaccaa ggcactctta cgtatcaagc ccgcagtccc
    13621 tctgcggtat gtgcccgtgc cgcgcgaggt gagggaacga gcctacgaag tgagtcccgc
    13681 cttcagggag ttacgaccgt tcttcgaagg caatgtcggt cgcacatggg cgagtttcgg
    13741 gaaagacgcg ggcgtgcagg cagaggggga gatttccgcg gggcacgtct ggtgggcgct
    13801 caaccagggc acttaccaga ttgggtataa caagtcggcg cgccaggcgg ggcgattcta
    13861 caagcgtatc gcggacgaag tgaacgccgc ctgtgacgac ggtcgcctgg catgtcgcac
    13921 agtattctca agtttgattg acccgcacgt gcaaaattat tggcctcacc tccccggctc
    13981 gttggtgcgg atgacgagtc tcttcacttc cacggctgaa gtgccgaagc cgcgggacgc
    14041 taatcacctg ccgccagacc tccgcgtcct cttcgactcg gtgacgaacc gccggacggc
    14101 gttgcaaaac cctcctgcca tgagaatcag gggctgggcg ttcaatcccg gtgataaact
    14161 tcagggcgtg ttggtacgca atcaggacgg gcgcgtcttg gcgggcgggc gattaacccc
    14221 gcgccatgat gtcgccgccg cattcacttc ccagaatggc accgcgcctc tcgataccgg
    14281 attcgacctt ttgctccccc cgtcgtcgcc catgccctat aacgcagacc tcgtcttcac
    14341 gaccgggagt ggcaaagaac taatcgtccg gcggccgggg ggggccgccg actcgccggg
    14401 gccggggctt acttaccacg ttgatgagga catgactttt cccgactcgc accggttgga
    14461 gttggccacc cagagctaca tctggagcgt ccacgggaag atcatcatgc tgctcactta
    14521 cgcggggttg tgcgcgctgc tgctcgcgct gatttttttt cgctcggtaa agctcaggga
    14581 accagccgtc ttcgtgtcgg tgcttctggc ggcggctatc gtcagccggt tggcgctctt
    14641 tactttgata gacgctgcgg catatatcgc cgctgaaata cgctatatat ttcctgtgac
    14701 ctatctttac acctgcctac tcctgatact catccactat tcgctgtccc tcgtcacagc
    14761 tcgcttggac ggcagcagcg tattacagag aattgatatt agaaagttgt taaaaaaaac
    14821 gatggagaaa tccgcgcggt ggaagcagga aaagccgagg accggggaat cccgccagca
    14881 agtaggatga gagaactttg cgagtcaaca tcccggcgaa aaacaggaag ccggatgtcg
    14941 cccgccccgt ccgcgtcgag tctggagtca aagtgtttgt cgtgacgccc tgcccgagcg
    15001 aggcagagat gctggggacg tggcgacctt acaccgagcg ggcacccaag tgctttacca
    15061 acattaaggg cggaggcgtc gccagtaaac gccggcccaa tctatctcga ctatctcctc
    15121 cgtgatgcca tgtacccggc ggtaatcgtc caccgccggc ttgcacgccg gcacggcctt
    15181 atagtcgtca atgatgatga agcccccggc cgacagcttg gggtaaaggt ttgtcatcca
    15241 tcaacagttg agaactgaag tcccgtggcg tagcgcttat gatgaccggc gatagaggtt
    15301 taagcacctt cacatatccg ctctcgacgt agaccttcca cgtccctgga tcagtgaagg
    15361 cataggtctc cttcaacaag tagcggttgc aggagtgcgc cgccccgttc tatcgctcca
    15421 acattgaggt tgagatgtgt gtcgagtcgg ctaccgggct tcatcggcga gcacttcgct
    15481 gtctgccaca cggctgaacc actcttcgat tatcggaatt tccgtgggag gaatcacctc
    15541 cgcgtcgctg agcgcgcgcc acaaggttaa ggccgtggtt gacaggtctg acgtgttcgg
    15601 ctcaagaccg gaaagcgtgt gggtcagtat ctcgccgatt tcgcgattcc ttaaataccc
    15661 gaccacctcg tcagaaaaat ctttcatcaa gtcgtgttca ttccgcaact gctcgaccgt
    15721 gggtgggtgg aatgagacgg acaggccgtg atgcgccagc gccgcctggg cgacgaacga
    15781 tctccagatg tcggtcatcc tgaaacttac gaaacacggc aggtacagca gggggaaagc
    15841 ttcgcggaag aagacggtgt tctgtgaatt aaaaggcacc cagacatcct tcgccagcac
    15901 gaccggccgg gcctcgcgct cgaagaacac cgggcgctta ttcgttaatc ggtagacggc
    15961 gtcaacgtcc gggtcgttgt ccgcaagata ttgttggatc gggcacggcg aagacgtcgc
    16021 cagcggagta acccggccac ggttatcgat ggcgtccaga ggtaaacccc gaggccagat
    16081 gtgcgcgtca tcggtgaaat gcttataaat gttcacccaa cctgggccgt caaccaaccg
    16141 cccgctgacg ctgcgactta actcctgacc gaaggtgtca tagggaatat tatcgtcatc
    16201 ggtttccagg atgagttcgg cgccctgccg gatcgcatgg aggtatccga ggttcttgcg
    16261 ggagtaatgt ttgtagggga tcgcgtcggc gaacgtcccg aacattgttc gctgggcggc
    16321 cacgtcgagg tatgtgacgc cgtcgcaatg ccaacgagga ggtgttttgg tgtcgccaac
    16381 gacgaccgca gaccagccgc ggtcgcatag acgggcaatc gactcgacag ccttcgtcgg
    16441 aggattaatt gtggtcagta ctatccagga atttttcacg ccgcaggctt tccttcgaag
    16501 cgattcttaa ctctttcccc tcggcactcc ggaggcccat cgcgatctcg cgccaaagca
    16561 gcttgagttc ggcgatgccg gtgggaaggc tggcaaaatt agtcgcgccg tcgattcgcg
    16621 gccactcatg tgtggggaac tcggtgattc tgattcccag tttgagtgcc cggatgacca
    16681 tctggtaatc catcgtcagg tcgcgcgaag ataaattcat ccgatcaaat gtctcgcagg
    16741 tgatcccgcg gaagccgtgt gtgacatcgc tggttcggtt accgccatgc gcgaaaaaga
    16801 cgttggcgag gagggcgaag ccctgattgg catacttgcg ccatttgaaa accttatcat
    16861 cctcttcatt ccaggcgccc tgaatcatcc gcgaagcgac cacaaactcg tgccccgcac
    16921 gcagcaattc agccatgcga ggcagatcgg ccgggtcttc attgccgtca ggatgaaagt
    16981 agatgaagga atgagtcttc acgcgctcac gcgcctcgat catcgcggag ccgagaccac
    17041 gtttcacttg cgggtacgct tgatacccaa aggacttcat caactcgaca cttttatcag
    17101 tcgaagtgcc gtcgatggca aaaaagtcgt cgaaaatatc ggtggaaatt ttagggagga
    17161 cttggcgaag cccgacctct tcatttcgga ctaccaaaat cagcgaaacc gtcttgtcat
    17221 cgatctcagt cacgaaacat tccccttctc accgacgcgg ttaacatgct gacgcgtgct
    17281 gtttatgaag cctccacaga atccggggag cgcgagatgc tgacacagca cccgaacacc
    17341 ggcccaaccg atgtgaggaa actatctgag aattttagtt aaccgctcgg aaaacgaaac
    17401 gggcgagcaa tccatttcaa cccgcttcag cgcgttctcg cgcagcaacg tccagagccg
    17461 ctcatcgcgg taaagcctga tacaggccga cgcgaaaccc ttcgcatcat ccgccaacag
    17521 aacttcctct tcgtgcgccc agccgagttg gccgccgatg agtgtcgtcg ccactacggg
    17581 caggccatac gccgccgcct cgtgaacctt gtaggggagg ccggccgaga agcgcgtcgg
    17641 ggcgacgaag agacgcgcgc ggtcgtagag ctccgtcaaa tcctcgacgc gccccaaaac
    17701 tttcacgtgg ccgttgtcga ggccggcgaa gaagtcgcgc gaggcgtggc cggcgacgag
    17761 caggcgcacg tcttcgccga gcgacttctg gatgagcggc agcacactct tgccgaacca
    17821 ggcgacggag tcggcgttgg gcgagtcggg gtcgtggatg gcgccgacga agaggatgtc
    17881 gcggcgctcg tcgaagccgc gcggcgtggg tcgcgcgttg accgtgtggc cgagcgtgtg
    17941 gacgttcgag aagccgcgtc gggcgaactc tcgcccctcg ccgtcggaga cggagacgac
    18001 cgcgtcgcag cccacggcga ggcccacctc ctcggacact tgcccctcct tctcccgctc
    18061 ggatggcgcc ctgccccgca gccgcaagcg ttcggcctcc ctgagcgaga agagcgcctc
    18121 ggcgtcgtag accaccttcg cgccggccag aagttgcggg tgacgctcca gcagcgcctt
    18181 gagcgcggcc atgttgtgcg ggcggctgac gtagacgagg tcgtagtagc ccgccctctc
    18241 ggccagaaac tcctccagcc cggcgcgccc gcgcccgagc atgacctcca cttcgcgcgg
    18301 gatgtcgccg tagacctcgg cccactcctc gtgcggctgg tcgagcgggt agcaggtgac
    18361 gcagtggccc atcgcgacca tctccgagac gatgcggttg ctgcggggga agcccgagcc
    18421 gagatgtgcg tggggcacgc ggtcgtcgag gtagaggacg cgcctgcacc ggggcagcgc
    18481 gtgtgtgcgc gccgcaagca cgttagaggg cgagaagggc tgttgcgcgc tcagccactc
    18541 gcggtgctgc gcggcgaaga cgggctggtg acgcgactgc atctcaagcg cgcgccggaa
    18601 tgagacggcg ctgccgaact cgtagtggag cacggagacg cgcgggtcgt agacgacacg
    18661 cttgccgcgc ttccagagcc tgacgcagta gtcggcgtcc tcgtagtagg ccggggcgaa
    18721 ggcttcgtcg aagccgccgc cttcgaggaa gagttcgcgc cgcgtcagga ggaagacggc
    18781 cgagcagtag tccacgtcgc ggcggaagag aaactcgggg gcgttggggg cgccgccgcg
    18841 cccgtagccg tggcacgagc cgtcctgcca gacgatgctg ccggcctcct gaagcgtcac
    18901 gtcggggagg atgatgcggc cgccgacggc gccgatgtcg tcagacgagt cgagcgtctc
    18961 ggtggcggcc gcgacggcgt cgttgaggag ctgcgcgtcg ttgttgagca gcaggaggtt
    19021 gcgcccgcgc gcgagccggg ccgcctgatt gcaggcgcga agatagtgga cgttggtctc
    19081 gttgcggacg acgcgcgcgc ccctgacgcg gccgagcagc gcgggggtct cgtcggtcga
    19141 ggcgttgtcg atgatgacga cctcgtaggg gtctttgtgg ttattgagta tcgattgcag
    19201 gcacttgagg gtcagctcgg cgcggttgta gaggacgagg atgatgctcg tcgcgggcgt
    19261 ttcgtgctgc gggaactcga tgacggagtc ggagctgaaa aagatgccca gcgaaaactt
    19321 gcacatcgac tccagcgtct tcttcgagaa tgagaggctg tcctgcgcga gcagggagaa
    19381 cgcatccgtc cgtgcccatg tcccgtcgcc gggcgctggc tccgcgcccg gcccggcggg
    19441 tcgctcggcc gggccgggca cctcgaccat ctgctcgtag tcctcgatgc gcccgcgctg
    19501 atccgtgacg cggacggtaa gccgccggcg gccgacgaac aaagactcgt ccaccgtcaa
    19561 ccgcccctcg tagccgcact tgaccggcgc gcgcgaaggg tatggggcca cgtccagacg
    19621 cgtcttcccg taacacaacg gcccgaggct gatcgtgtcg agaaaggcct cgacgcgcac
    19681 cacggccgcg ccctcggagt aggcccagcc cctgatctcg atctcttgtg aaatctcatc
    19741 gacttcggcg ggctgctcca gcgcgccgtg gaaggatgcc ggctggagac tgccgaggcg
    19801 cgtgcgcaag cgcaggagca ggaagttgag gcggcgcagc catgaggtgt agcgccagga
    19861 gcgcgacagg gtaatccaac ggagcgcctc cgcctgcgcc ttgacgcgcg cgcgtagctc
    19921 cgtcaacacc tgcgcctgat gcatccgcgg gcgggactcg actgaatcgg ccgccgcgcg
    19981 cgagccagcc gcgcattccg gccatgcctc gggcagagcg ccgtgcgcct cctcaggcgg
    20041 cgcggcgctc gccggagggg gtagcgcgag gccgttgctt tcgggaggcg ctccgccggg
    20101 caatcccccg gacggtgatt cgtcgtatgc ttgtattggc ttcatccgta cactgccggg
    20161 gagactaaaa tcaggcacgt tcgcgggagg tcctactgac gggcgcacca gccgtgccag
    20221 ggaggagtta aaccctggtt cgcgccgggt gaaatgagat gagccgagcc ggactcgaac
    20281 cggcgacccg ctggttaaaa gccag
//
LOCUS       DASVES010000486         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_303064, whole genome shotgun sequence.
ACCESSION   DASVES010000486 DASVES010000000
VERSION     DASVES010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303064"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VF240_20300"
     CDS             complement(<1..840)
                     /locus_tag="VF240_20300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7177612.1"
                     /translation="MTDDAKQVTQPQTMPEDLRASEIRYRRLFESARDGILILDADSR
                     KITDANPFMVEFLGYSWDEFLGRELWEIGLFKDKDESQTAFRELQATGYIRYEDMPLQ
                     TKAGKQWNVEFISNVYREDGHQVIQCNIRDITARKSAAEALAQANQRVADILESITDA
                     FFAVDHEWRFTYLNRQAEPLLQRKRDEILGKSIWEEFPEAVDSVFAVGSVFHQQYHRA
                     SADQVTVNFEEFFPPLDTWFEIHIYPGSAGLSVYFHDIGARKQAEREMKVSEVRYRRL
                     FEAA"
     gene            1045..2520
                     /locus_tag="VF240_20305"
     CDS             1045..2520
                     /locus_tag="VF240_20305"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7177613.1"
                     /translation="MTSEKEKKEPLRLLIVDDEEAARYGMRRALLDHAGVIDDASSIG
                     DAREKIRALSPDLILLDVNLPDGTGLDFLRELSGAGEEMPPVILITAYGSERMAVEAI
                     KAGAYDYLSKPFEVDDLRLCVKNALETVRLRRENERLRKRIERETAQQGTLLGNSVAM
                     QRVRELIDKVADTDATVLVRGESGTGKELVARELHERSTSRRRGAFVAVNCAALPTEL
                     IESELFGHEKGAFTGAAARRAGKFEQAGGGTLFLDEIGDMSANVQAKLLRALEERKIE
                     RLGGGESIPVDVRIVSATHRPLEQDIAAGSFRADLFYRLRVVTIDIPPLRERREDIPA
                     LAETFARLACERYSLPRRTVGVAALRRLVEYRWPGNVRELKNAVERSVILAEGDELTA
                     RDLPEEIKAGPGASAETGVEGGASGALDVPFTADFREDRREFERRYIARCLEETGGNV
                     TRAASILGMHRQSLQHKLRELGMARRYVSVGQDDAQEKSDN"
     gene            2543..3199
                     /locus_tag="VF240_20310"
     CDS             2543..3199
                     /locus_tag="VF240_20310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX7177614.1"
                     /translation="MKAKIDAILRPEQAEYLERLLPDNTGLLEEMEHYAAAHGVPIAD
                     REVARFLEITARATGARRVLEVGMAIGYSVIHFARALPTGGLVVTIEPSEKMITAAEG
                     FIERAGLRERVQVERGYALEVIPRLEGPFDILFIDALKEEYAGYLDLALPLLRAGGTV
                     IVDNLLWGGQVAGEIRSPDQTASTQAIRAFNQTFVRHPQLLAEVLPLGDGLGYGVKLD
                     "
     gene            complement(3221..3700)
                     /locus_tag="VF240_20315"
     CDS             complement(3221..3700)
                     /locus_tag="VF240_20315"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX7177615.1"
                     /translation="MSGIIVKTFIGSTRLCTRAARLVLREPGKALLLARMTAWVGTLS
                     LLIRALPLPMAMRALTPLRRRAPSHADAAILEARIAELLDMLLAADVLFLTPTCWKRA
                     PVLYRFLALEGIETRVVFGVRKESQGLLAGHAWLEAGGRPLFEKTLPDYKVTFSFPA"
     gene            3725..>4232
                     /locus_tag="VF240_20320"
     CDS             3725..>4232
                     /locus_tag="VF240_20320"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7177616.1"
                     /translation="MLTTILFDNAESRARAGLLVASGGVLAFRTDTFYGLGADPFNPD
                     ALNKLNELKGRDSKPILLVISDLPMVARFVRFTSPLFPLLAGRHWPGPLTLVAEAHER
                     VPLEVTAGTGTIGVRLPDDEEVRGFVRACGGALTATSANPAGEPPARTAQEAARYFQD
                     RLELVVDGG"
BASE COUNT          814 a         1317 c         1335 g          766 t
ORIGIN      
        1 ggctgcttca aagagccgcc ggtaacgaac ttccgaaact ttcatttcgc gctcggcctg
       61 cttacgcgcg ccgatgtcat ggaaatagac cgacagtcca gccgatcccg gataaatatg
      121 aatctcgaac caagtgtcga gcggaggaaa gaactcctcg aaattgaccg ttacttgatc
      181 tgctgacgcc cggtgatatt gctgatggaa gacggaaccg acggcaaaga cggaatcgac
      241 cgcctcaggg aattcttccc agatactctt gccgagaatc tcatcgcgct tgcgctgcaa
      301 gagcggctca gcctgccgat tcagatatgt gaagcgccac tcgtgatcga cagcgaagaa
      361 ggcatcggtg atgctttcca gaatgtcggc gacgcgctgg tttgcttgcg ccaacgcctc
      421 cgccgccgac ttgcgcgcgg tgatgtcgcg gatgttgcat tggatgactt gatggccgtc
      481 ttcccgatag acgttgctga tgaattccac gttccattgc tttcccgcct tggtctggag
      541 cggcatatcc tcgtaacgga tatagcccgt cgcctgcaac tcccgaaacg ccgtctggct
      601 ttcatcctta tctttaaaca gaccgatctc ccagagttct cggcctaaga attcatccca
      661 tgagtagccc aagaactcca ccataaatgg gttcgcgtcg gtgatcttgc ggctgtcggc
      721 atcgagaatc aggatgccgt ctcgcgcgga ttcaaacaga cgccggtagc ggatttcgga
      781 agcccgtaag tcctccggca tggtctgagg ctgggttacc tgcttggcat catcggtcac
      841 ttgagaaatc tccacttcat tagcgagtcc agagcgacga gattcgacga gggggaattc
      901 aggccgttgt cctttgaggg tgcttcaata atacgccagg aaaaggaatc atttcaaact
      961 gataagtgtt agtgttgcgg gtcacacgcg gcgtcgaggc ttctggcacg ttacgtgctc
     1021 tgatctggta acatggacaa tctgatgact tcggaaaaag agaaaaagga accccttcgg
     1081 ctgctcatcg ttgacgacga ggaggccgcg cgctacggca tgcgccgcgc cctgctcgac
     1141 cacgccggcg tcatcgacga cgcgtcgagc atcggcgacg cccgcgaaaa aatccgcgcc
     1201 ctgagtcccg acctgatcct cctcgacgtt aacctccccg acggcaccgg actcgacttc
     1261 ctgcgcgagc tgagcggcgc gggtgaagag atgccgcccg tcattctcat tacggcctac
     1321 gggtcggagc ggatggccgt cgaggccatc aaggcgggcg cttacgacta tctctcgaag
     1381 cctttcgagg ttgacgacct ccggctctgc gtcaagaacg ctctggagac ggtgcgtctg
     1441 cgacgcgaga acgagcgtct gcgtaagcgc atcgagcgcg agacggcgca gcagggaacg
     1501 ctcctcggaa actcagtggc catgcagcgt gtgcgcgagt tgatcgacaa ggtcgctgat
     1561 acggacgcta ccgtcctcgt ccgcggcgag tcgggcacgg gcaaggaact cgtcgcgcgc
     1621 gagctgcacg agcgcagcac ctcccgacga cgcggcgcat tcgtcgccgt caactgcgcg
     1681 gcgttgccca ccgagttaat cgaatccgag ctattcggcc acgagaaggg ggcgtttacg
     1741 ggggccgccg cgcgccgggc ggggaagttt gagcaggccg gcggcggcac gctctttctc
     1801 gacgagatcg gcgacatgag cgccaacgtg caggctaaac tcctgcgcgc gctcgaagag
     1861 cgaaagattg aacgtctcgg cggcggggag tcgatccccg tggacgtgcg tatcgtgagt
     1921 gcgacacacc gaccgctcga acaggacatc gccgccggca gcttccgggc cgacctcttc
     1981 tatcgcctgc gcgtcgtcac gattgacatc cccccgctac gcgagcgacg cgaggacatc
     2041 cccgcccttg ccgagacgtt cgcgcggctc gcgtgcgaac gctacagcct gccgcggcgc
     2101 accgtgggag tcgcggccct ccggcggctc gtcgaatatc gctggccggg caacgtccgc
     2161 gagttgaaga acgccgtcga gcgttcggtc atcctcgcgg agggcgacga gttgaccgcg
     2221 cgcgacctcc ccgaagagat caaggctggc cccggagcga gtgcggaaac gggggttgag
     2281 ggcggagcgt ccggcgcttt ggatgtcccc ttcacggctg acttccgaga ggatcggcgg
     2341 gagttcgagc gccgctacat cgcccgctgt ttggaggaga cgggcggcaa cgtgacgcgc
     2401 gccgcctcca tcctcgggat gcaccgacag tcgctccaac acaagctgcg cgagctgggg
     2461 atggcccgcc gctacgtgtc ggtcgggcag gacgacgcgc aggaaaaatc cgacaactga
     2521 agtgagtatt ttctagtgag acatgaaagc taaaatcgac gccatccttc gccccgagca
     2581 ggccgaatat ctcgaacgcc tgctccccga caacaccggc ctcctcgaag agatggagca
     2641 ttacgccgcc gcgcacgggg tgccgattgc cgaccgggag gtcgcccgct tcctcgaaat
     2701 cactgcgcgc gcgacaggcg cccgacgcgt gctcgaagtc gggatggcca tcggctactc
     2761 ggtcatccac ttcgcgaggg cgctgcccac aggcggcctc gtcgtcacca tcgagccgag
     2821 cgaaaagatg attacggctg ccgaagggtt catcgaacgg gccggactgc gcgagcgagt
     2881 ccaagttgaa cgcggctatg ccctcgaagt aatcccgcgc ctcgaaggcc cctttgacat
     2941 tcttttcatc gacgctttga aggaggagta cgcgggctat cttgatttgg ctcttccgct
     3001 cctgcgcgcg ggcggcacgg tcatcgtcga taacctgctc tggggcgggc aggtggccgg
     3061 cgagatacgc tcaccggatc agacggcctc aacgcaggcc attcgcgcct tcaaccaaac
     3121 tttcgtccgg cacccacaac tactcgcgga ggtgctgccg ctcggcgacg gtctcggtta
     3181 cggcgttaaa ctggactgat tggagtggcg cggcggatgt tcaggcggga aagctaaacg
     3241 taaccttgta atcgggaagc gtcttctcga agagcggtcg cccgcccgct tcaagccacg
     3301 cgtggccggc gagtaggccc tgactttctt tcctgacgcc gaagacgacg cgcgtctcga
     3361 taccttcgag ggcgaggaag cggtagagaa cgggcgcgcg cttccagcag gtcggggtga
     3421 gaaaaagaac gtcggccgcg agcaacatgt cgagcaattc ggcgatgcgc gcttcaagta
     3481 tggccgcgtc tgcgtgcgac ggggcgcgtc gccgcagggg cgtcaacgcg cgcatcgcca
     3541 taggcagcgg tagcgccctg atgagtaggg agagcgtccc gacccatgcc gtcatgcgcg
     3601 ccaaaaggag ggccttcccc ggttcgcgca ggacgagacg cgccgcccgc gtgcagaggc
     3661 gcgtgctccc aataaaagtt tttacgataa tgccagacat cggatgtgta gggcgtgcgg
     3721 cgaggtgttg acaacaattc tttttgacaa cgctgaatcg agggcgcgcg ccgggctgct
     3781 cgtcgcctcg ggcggcgtcc tcgcgtttcg cacggatacg ttttacggcc tcggagctga
     3841 ccccttcaac cccgacgccc tcaataaact gaacgagctg aagggccgcg actccaagcc
     3901 catcctcctc gtcatcagcg acctgccgat ggtcgcccgg ttcgtccgct ttacctcgcc
     3961 cctgttcccc ttgctcgcgg gtcggcactg gccggggccg ctgacgctcg tcgccgaggc
     4021 gcatgagcgt gttcctctgg aagtgaccgc gggcacggga acaatcggcg tacgactccc
     4081 cgacgacgag gaggtgcgag gcttcgtgcg cgcctgcggt ggcgcgctga ccgcgacgag
     4141 cgcaaacccg gcgggggaac cgcccgcccg cactgcgcaa gaggcggcgc gttattttca
     4201 agatcggctc gaactcgtcg tcgatggagg gc
//
LOCUS       DASVES010000487         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_489949, whole genome shotgun sequence.
ACCESSION   DASVES010000487 DASVES010000000
VERSION     DASVES010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489949"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..729)
                     /gene="uraD"
                     /locus_tag="VF240_20325"
     CDS             complement(175..729)
                     /gene="uraD"
                     /locus_tag="VF240_20325"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX7177617.1"
                     /translation="MSESDTEGLGAGGRSSVERLNALSGDEAFARLLECCGSTRWAQS
                     MVVRRPFADERHLIEVAERIWWGLTSDDWLEAFHAHPKIGEARPARETGEGAQRWAEE
                     EQAAARAAASATLDALASANQSYEQRFGHIFIVCATGKSSEEMLETLRRRLSNDPGTE
                     LCVAAEQQRRITLLRLRKLLTSDK"
     gene            complement(732..1739)
                     /gene="alc"
                     /locus_tag="VF240_20330"
     CDS             complement(732..1739)
                     /gene="alc"
                     /locus_tag="VF240_20330"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEX7177618.1"
                     /translation="MDFTELVDLASERLGGSVIYANDEFFAPKENLLRPSAPVFIEGK
                     YTERGKWMDGWETRRRRTPGYDWCIVRLGLPGLVRGLLVDTAFFRGNYPAQCSLDACV
                     VADFRDEHLFNLDEEGLWTQILPVTDLQGDTQNFVPVRGTEAFTHLRFKIYPDGGVAR
                     LRVYGEVLPDPSLLREAGEVDLAAVENGGMVVAASDMFFGHRHNLIMPGRAVSMSDGW
                     ETQRRRGEGNDWVVVKLGAKGNISRLEVDTAHFKGNYPESCSLEACDASGRAVEGEST
                     QALPWREVLPRVRLQAHTRHHFADSIKAAGAVTHVRFQIYPDGGVSRLRVYGRAGEGE
                     S"
     gene            complement(1925..3286)
                     /gene="allB"
                     /locus_tag="VF240_20335"
     CDS             complement(1925..3286)
                     /gene="allB"
                     /locus_tag="VF240_20335"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX7177619.1"
                     /translation="MTSSEFIIRGRTVVTPGGVAAAAIRVGGGRVVGVGEFDEAAGGR
                     EVVEVEDRFVLMPGLVDSHVHVNEPGRTEWEGFETATRSAAAGGVTTIVDMPLNSIPP
                     TTTAGNLSAKRAAAEGKCHVDVGFWGGVVPGNVAELRALHAAGVVGFKCFLIDSGVEE
                     FPHVGEDELREALGELASMGATLIAHAEVPGPVESASALAAGEARSYGTFLSSRPRAA
                     EDEAVALLVRLCREMGARVHVVHHSSADALPLLRAAKEEGLPVTAETCPHYLSFAAEE
                     IPDGATEFKCCPPVRERENRERLWDALGSGTLDMIVSDHSPCPEALKLREEGDFMRAW
                     GGIASLQLRLPATWTEARKRGFDIEDLARWLCDAPARLAGFDGRKGRIAEGFDADFVV
                     WDPEAEFRVEPSVIRHRHKLTPYLGRTLSGVVQATYLRGEKIYERGRDADGPPTGRLL
                     ARA"
BASE COUNT          589 a         1180 c         1074 g          596 t
ORIGIN      
        1 cgcgccaccc tccgcctgca cttcgagaag caccgcgact ccggccgccg gtctcccccg
       61 cgacgtgtcg agtacgtgtg tcgtgatcgc gctcatatcg ttgggaagtg ataagtgatg
      121 agtgataagt gataagacaa aaactgtctt ctctttctca tcacttatca ctcatcactt
      181 atcgcttgtc agaagtttcc ggagacgcag gagcgtgatg cggcgctgtt gttcggcggc
      241 gacacacagt tccgtgccgg ggtcgttgga gaggcggcgg cgaagcgtct ccaacatctc
      301 ttcggaactt ttccccgtcg cgcagacgat gaagatgtgg ccgaagcgct gctcgtagga
      361 ttgattggca gaggctaacg cgtcgagggt ggcggaggcg gcggcgcggg cagccgcctg
      421 ctcttcctcc gcccagcgtt gcgccccctc accggtctcg cgcgcgggcc gggcctcccc
      481 gattttcggg tgggcgtgga aggcttcgag ccagtcgtcg ctcgtgaggc cccaccagat
      541 tctctcggcg acttcgatca gatgccgctc gtctgcgaaa ggtcggcgca cgaccatcga
      601 ctgcgcccag cgcgtcgagc cgcagcattc gagcaggcgc gcgaacgcct cgtctccgga
      661 gagcgcgttg agacgttcca cgcttgagcg cccgccggcc cccagccctt cagtgtcact
      721 ttcgctcaac tttagctctc accttcaccg gcgcggccgt agacgcgcag tcgcgacacg
      781 cccccgtcgg ggtaaatctg aaaacgcacg tgggtgacgg cgccggccgc cttgatcgag
      841 tcggcgaagt ggtggcgggt gtgcgcctgc aagcgcacgc gcggcagcac ctctcgccac
      901 gggagcgcct gcgtagactc accttcgacg gcgcgcccgc tcgcgtcgca cgcctccagt
      961 gaacaactct ctggatagtt gcctttgaag tgtgcggtgt ccacttcgag gcggctgatg
     1021 ttgcccttcg ccccgagttt gacgacaacc cagtcgttgc cctcgccgcg ccggcgctgc
     1081 gtctcccagc cgtcgctcat cgagacggcg cggccgggca tgatgaggtt gtgccgatgt
     1141 ccgaagaaca tgtcgctcgc ggcgacgacc atgccgccgt tctcgacggc cgccagatcg
     1201 acttcgcccg cttcgcgcag aagcgacggg tcgggcaaga cttcgccgta gacgcgcagg
     1261 cgcgccacgc cgccatcggg gtaaatcttg aaacgcaggt gcgtgaaggc ttccgtgccg
     1321 cggacgggga cgaagttctg cgtgtcgccc tgaaggtctg tgacgggcag aatctgcgtc
     1381 cacagtccct cctcgtcgag attgaacagg tgctcgtcgc ggaagtctgc gacgacgcac
     1441 gcgtcgagcg agcactgcgc cgggtagttg ccgcgaaaga aggcggtgtc cacgagcagg
     1501 ccgcgcacta gccccggcag gccgaggcgc acgatgcacc agtcgtagcc gggcgtgcgg
     1561 cggcggcgcg tctcccaccc gtccatccac ttgccgcgct ccgtatattt gccttcgatg
     1621 aagacgggcg ccgacggccg caacagattc tccttcggcg cgaagaactc gtcgttggcg
     1681 tagatgaccg agccgcccag ccgctccgag gcgaggtcaa ccagctccgt gaaatccatt
     1741 catcctccct gtgtgaagtt atcagctatt agctgttagc tgttagctgt tagccagaag
     1801 cgggagcgag gggtatgcag ccgtcgatgc aactaaactc ctgttcgctc gcctctggct
     1861 gaatgctaac agctaacagt taatagctga ccgctatcaa ctggctacta accatctgcg
     1921 actcttacgc tcgcgcaagc agtcggcctg ttggcgggcc atcggcgtcg cgcccgcgct
     1981 catagatttt ctcgccgcga agatacgtcg cctgcaccac gcccgacaat gtgcgaccga
     2041 ggtacggcgt gagcttatga cggtgccgga tgacggatgg ctcgacgcga aactcggcct
     2101 ccgggtccca gacgacgaag tcggcgtcga agccttcggc gatgcgcccc ttgcggccgt
     2161 cgaagccggc aagacgcgcg ggcgcgtcac aaagccaccg cgcgaggtct tcgatgtcga
     2221 agccgcgctt tcgcgcctcc gtccacgtcg ccggcagacg aagctgtagc gacgcgatgc
     2281 cgccccacgc gcgcatgaag tcgccctcct cgcgcagctt tagcgcctcc gggcacggcg
     2341 agtggtcgga gacgatcatg tcgagtgtcc cggagcctaa agcatcccac agccgttcgc
     2401 ggttctcgcg ctcgcgaacg ggtgggcaac atttgaactc cgtcgcgccg tcgggaatct
     2461 cttccgccgc gaagctaaga tagtgcggac acgtctcggc ggtaacgggc agcccctcct
     2521 ccttcgccgc gcgcaggagc ggcaaagcgt cggccgagga gtggtgaacg acgtgaacgc
     2581 gcgcgcccat ctcgcggcac agtctgacga ggagcgcgac ggcctcgtct tcggccgctc
     2641 gcgggcgcga actcaaaaac gtcccgtagc tgcgcgcttc gccggccgcg agcgcggacg
     2701 cgctctccac cggccccggc acttcggcgt gcgcgatcag cgtcgccccc atcgaagcca
     2761 gctcccccag cgcctcgcgc aattcatctt ccccgacgtg cggaaactcc tcgacgcccg
     2821 agtcgatcag gaagcacttg aacccgacca cgcccgccgc gtggagcgcg cgcagctccg
     2881 cgacgttgcc cggaaccaca ccgccccaga agccgacatc gacgtggcac ttcccttccg
     2941 ccgccgctcg tttcgcgctc agattcccgg ccgtcgtcgt cggcgggatg ctgttgaggg
     3001 gcatgtccac aatcgtcgtc acgccgcccg cggcggcgga gcgcgtcgcc gtctcgaacc
     3061 cctcccactc cgtccgcccc ggctcgttca cgtgtacgtg cgaatccacg aggcccggca
     3121 tcaagacgaa tcggtcttcc acctcaacga cctcgcgccc gcccgccgcc tcatcgaact
     3181 caccaacgcc gacaactcgc ccgccgccga cgcgaatcgc agccgccgcg acgccccccg
     3241 gcgtgacgac cgtgcggccg cgtatgatga actcggacga agtcatcctt gcaatgttaa
     3301 agagacctgt aggggcaggg cttgtccctg cccgtgctta cagaagaatt aacatggatg
     3361 ggcaggatgt gcaggatgaa gcaagcgctg attggagatt tcaacggggg cgcgcagggc
     3421 agggcagtag cccgaccgt
//
LOCUS       DASVES010000488        21325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1478408, whole genome shotgun sequence.
ACCESSION   DASVES010000488 DASVES010000000
VERSION     DASVES010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21325
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478408"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1999
                     /locus_tag="VF240_20340"
     CDS             212..1999
                     /locus_tag="VF240_20340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX7177620.1"
                     /translation="MHPNLRSFLQTLTREDDLLTVEAEVDPYLELAEVHRRVIERGGP
                     ALLFRKVKGSRYPVVTNLFGTERRIGLAFGPKPEALVRRAVEIAESLLPPRPKELWRH
                     KSLALEALRLGTRRVRRAPVAEVVERPARLDELPVLTTWQEDGGPFFTLPLVYTEHPE
                     TLKHNLGMYRLQRFDSGSTGLHWQIHKGGGFHYHEAERAGQALPVSVFLGGPPALILA
                     AIAPLPEDIPELVLASVLAGERVAVAANPLGHPHRLVAEAEFALVGRVPPVERRPEGP
                     FGDHYGYYSLQHDYPVFNVEAVFHRKDAIYPATVVGKPRQEDFYIGDYLQRLLSPLFP
                     LVMPSVRDLWTYGETGFHSLCAAVVRERYGREALVSAFRILGEGQLSLTKFLILTDTP
                     QDLRDFPRLLEHVLARARFERDLFVFSNLSMDTLDYTSGKVNEGSKAVLLGLGDAVRE
                     LPREFRGRLSAGLRRAEVFCGGCLVVEGSSFAEDRELAARVAADAVFKDWPLIIIHDD
                     ASVAHKSADFLWATWTRFEPASDIHAAATTVERHHLSYSPPIVIDARMKPGYPDELVV
                     RPDVAELVSRRWDEYFPRGLNAEGSTSNV"
     gene            1992..3236
                     /locus_tag="VF240_20345"
     CDS             1992..3236
                     /locus_tag="VF240_20345"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7177621.1"
                     /translation="MSETSDPPRVMSVVEETSCVIVGGGPAGAVLSLMLARKGVPVML
                     LEAHDDFDRDFRGDTLHPSVLELMDELGLADRLLQLRHSKLRSANLATPDGTVTVANF
                     GRLRTRYPYITMLPQAKFLEFLTDEAKCYPEFRLVMGARVEELIEEDGAVRGVRYEGD
                     DGWHEVRAPLTVGADGRSSRVRRLSGLDADTIKTSPMMDVLWFRIPRRPDDPEGLLGR
                     FGRGHGVVMFDRDEQWQVGFIIIKGSFKEVRAAGIDALKESFAETAPEFADRTEHLTE
                     WKQFAMLSVESSRLKNWTRPGLLLIGDAAHVMSPVGGVGINYAIQDAVVAANVLTEPL
                     LRGDVRDEFLREVQRQREWPTRVIQAIQAGGQRAVVANALKSDQPFRVPLLARLLLRI
                     PLLRDIPARIIAFGVRRVHAKI"
     gene            complement(3233..4660)
                     /locus_tag="VF240_20350"
     CDS             complement(3233..4660)
                     /locus_tag="VF240_20350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177622.1"
                     /translation="MPRYENRVQLVLALVIGLLLLANLFTLTLVVLTPAARPARASVV
                     FTVFVVTLIVSVPSLLLLPRWLLKPYRQLVDAAERAPVEARRQVGEQDETQFVLNTFH
                     EVVAQLRSKQRELETLSAQASARAVSAEQFSERIVASVPSGLLAVDSNGRATLVNAPA
                     RVLLGIVGGTEARAYVELLGNAPEMSDLIESCLLTGEVFRREEVAARTEGGAVRRLGV
                     TVAPIELGEGRGALCLLTDITEVLQLREAVALKRNLESLGEMSAGLAHEFKNALATLH
                     GYAQLFQTLDIDEHGRAAAAALLHEVRGLSEMVTSFLNFARPKPLDPADVSLAELVES
                     CRAEVEKLYLELRVELRVEGEFPVVRADERLLRQALLNVMRNAAESVGDEATERRVVV
                     SGSRARGEGGREFAVVEVADTGGGIPEADLRRIFIPFFTTKSKGHGIGLALAHRVVTD
                     HGGTLTARNDARGGAVFRISLPLST"
     gene            complement(4759..5640)
                     /locus_tag="VF240_20355"
     CDS             complement(4759..5640)
                     /locus_tag="VF240_20355"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX7177623.1"
                     /translation="MPNFLSLLPSHDFLGLPPALALAFVAVLGGLIGSFLNVVIHRVP
                     REESIVFPNSRCPSCGASIRFYDNVPVLSWLALRGRCRDCRTHISARYPAVEFLTAAL
                     FGLTFWFRSGLTLSLPFDLVFVAALVALIFIDAEHMLLPNVITYPGLALALVARVVVP
                     NLYGVEFLTRGSLAGWPVWLVSLVGALLGALVGGGFLWLAGWLWERARGVEAMGLGDV
                     KMMFMVGAYLGWPLTLLTIFIGVLGGSLAGVALMVRRGERDTQMLLPFGIFLGAGALV
                     SLLFGTQIADWYASQFR"
     gene            5787..7271
                     /locus_tag="VF240_20360"
     CDS             5787..7271
                     /locus_tag="VF240_20360"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX7177624.1"
                     /translation="MNSRKPIFALLAVGIAVVIALTFVPYAFLERAGRAVTAILPGAR
                     EATVDAPARPLLPAFNVEGWYAERGEDPARHGVHIESIDGKSVLASHNPDLPFNPASL
                     LKLATSLAVLRAFGPDHRFETRVYTDGVVGPDKTLKGNLVLAGNDPMFGDVAASLVAR
                     ELRARGVEKVSGELLVTPGFSFNYAEKADDSAERVAKVMEMKEKATGVGETPAGEPRF
                     VVKSYPLREILLYMNAHSNNFVAERLGALVGGPEGLKRFLVEELKLPAEQVEVERTSG
                     RENNRMTARGIVSVVRALKAEAERHGMRVEDLMPVASDDAGTLRRRLEGTPLEGATIG
                     KTGTLTAEVDGGMASLAGVVFTETAGMVAFAILDQGSRIAENRELEDILLAQVIASQD
                     IPRLIHSEEVRRRLLPATSLRVEPEALAGLVDERDDKKQTDERESDEEEASTSKSNAK
                     TRDGKRVEEKASQRSRKPEPSRADSRKREVQRGGRASVRGRGRR"
     gene            complement(7473..8255)
                     /locus_tag="VF240_20365"
     CDS             complement(7473..8255)
                     /locus_tag="VF240_20365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7177625.1"
                     /translation="MKVTATIITFNEAENIRAACESVSWADEILVVDSESTDATADLA
                     RGCGARVITRSWPGFAAQKQFAAEEARGEWVFSLDADERVSDELRASIDDLLHAPEAG
                     LADGYRIARRSFYMGRWIRGGGWYPDHQLRLYRKGRGRWAGAYVHESVKMSEGARVET
                     LAGDLLHYSVRDALHHHRMIGERYAPLAARQMYEAGRRTSPLKVALAGPTTFARGYVL
                     KGGFRDGLAGFAIARFAAHHAFLKHLLLWEMQNKEADRSDES"
     gene            complement(8380..10809)
                     /locus_tag="VF240_20370"
     CDS             complement(8380..10809)
                     /locus_tag="VF240_20370"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7177626.1"
                     /translation="MLSKGLRFLRHLCARPASRHVAALILAFAVMAPAPLHALSSDNA
                     RPSASSDAASTLTGTVRNIAGEAVASAAVYFRRTATNDVRTQTDDAGRFSFPDLRAGS
                     GTLMARAGDETAVAFVDLQPGANQVELTFQRKQFALAVAGAEAVPQSTPAPGPTSPAQ
                     RDATRPPGTEQRQPVPRDISPSPTNPTTPPGAQRPAPQTPPGTERAPQTPVTTPNVER
                     PTDAPTVTGTPLPETVPSAEQGTEQLLTLTQPRPLPPMPSLVRVGVVSGDNIPLTLNE
                     AIRRALENNNEIEVARTDVRLVESQLLSLRGVYDPVLTFRPEINNSVRPVVNIFGGAD
                     AAGTVATTDLNNDFSVTRQFGKGGGNYTYFFNNTRETTTASNNTLNPFYSSSQGLQFT
                     QPLWRNRSIDRNRQQIRIQQKRLEQSDADFRRRTIEIISQVQRAYWDLVFALRDEQNQ
                     IANLNLARENFRRTEASVAAGATAPLERAEIQTELSNRESALLLSSQSVTIAENNLKS
                     LIIKEPLSAEWSSVIVPTDQARFDDTPVSLETAIKEARDNRPELRRLKLQQDINDIDI
                     QFFRNQTKPQIDLTGTVSTTGLAGTPSASAGSGTRIVDLVGGDAVAATNADAFLFQSI
                     NRTRRDLGLDPLTTPQVEQGGAGVPSQFQGGYFQTLKNLFSLSTRNIVVGVTIQIPLR
                     NRTAEANLATVRIQREQLAANTRLQEQGVEVEVRNAVQSVETARRRVLAARSARENAE
                     LQLAGERRLYQVGRSTTFLLFQRENSLVNARNQELRAETDYNKALAELQRATSTTLRA
                     NNVIVDSPIGR"
     gene            11297..12304
                     /gene="gap"
                     /locus_tag="VF240_20375"
     CDS             11297..12304
                     /gene="gap"
                     /locus_tag="VF240_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7177627.1"
                     /translation="MAVKVGINGFGRIGRNILRTSLGDSEIDFVAVNDITDTKTLAHL
                     LKYDSVLGNLEHEIAATEKGIRVGSDEFTVFSERDPAKIDWESVGAEIVVESTGLFTN
                     AEDARKHLRGSVKKVIISAPAKNEDITIVLGVNEGSYDPAAHNIISNASCTTNCLAPV
                     AKVIHEKFGVRSAQMTTIHSYTNDQQLLDLPHKDLRRARAAALSMIPTSTGAAKAVAL
                     VLPELKGKFDGISVRVPTPNVSLIDVVIRVEKQTTTEEVNKALKDAANEELRGVLAFS
                     EEPLVSADFRKNPNSSIVDAEYTKVIDGDLVKILSWYDNEWGYSCRVRDLIKYIAGKG
                     L"
     gene            12391..13599
                     /locus_tag="VF240_20380"
     CDS             12391..13599
                     /locus_tag="VF240_20380"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7177628.1"
                     /translation="MPKLSIKDLDLKGKRVLIRVDFNVPIKDGKVEDDTRIEAALPTI
                     RHATSAGARVILASHLGRPKGERVEKYSLRPIAEHLSGLLGALVAFADDCVGEEARAK
                     IESLQNGDVLLLENLRFHAGEEKNDENFARELASLCDLYVNDAFGAAHRAHASTAGIT
                     KVVSQSAAGLLMEKELTYLGRVIHDPAHPFVAILGGAKVSDKIPVIDALITRKVDKLL
                     IGGAMAYTFFKADGFTIGKSLVENEMLDTAREIKKKAEDAGVELLLPTDHQVVDSYEP
                     VKGEQAVSKTIPIEFTNAGHVGLDIGVETVAHFSAALKDAKTIIWNGPMGYFEEKPFD
                     QGTIAIAQAVAEAADGGATVIVGGGDSVSAVTRAGVAERITHISTGGGATLEFIAGEE
                     LPGVTALNDK"
     gene            13706..14464
                     /gene="tpiA"
                     /locus_tag="VF240_20385"
     CDS             13706..14464
                     /gene="tpiA"
                     /locus_tag="VF240_20385"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7177629.1"
                     /translation="MRKPVIAGNWKMYKLVADSVATAQTLKSLVSNANHCEVVIAPVF
                     TALKTVADRLEGSNIRIAAQNCAAESKHGAHTGEVAADMLRDAGASHVIVGHSERRHI
                     YGETDDFVGRKVGAGLAAGLIVILCVGETLEEREGAQAEAVVRRHLETGLSGLTASDL
                     DRIIVAYEPVWAIGTGHTATPEQAQEMHAFARRVLAEKFGEEVAARVRILYGGSVKPD
                     NISKLMLQPDVDGALVGGASLEAESFARIVNYNS"
     gene            14481..14906
                     /gene="secG"
                     /locus_tag="VF240_20390"
     CDS             14481..14906
                     /gene="secG"
                     /locus_tag="VF240_20390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7177630.1"
                     /translation="MLIYVLYGIFILACLALILSVLLQPGKADAGSLFTSSFSSTAFG
                     PRGTQTVLAKISIVSAITFMLVALLISMGVGGYRSVIGSGDTATPVSEATPAPAAETP
                     AQEGAAQPNTNAAPDAPAAGDVPPAAATPAPAASPAPSS"
     gene            14970..15051
                     /locus_tag="VF240_20395"
     tRNA            14970..15051
                     /locus_tag="VF240_20395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15004..15006,aa:Leu,seq:gag)
     gene            15619..16686
                     /locus_tag="VF240_20400"
     CDS             15619..16686
                     /locus_tag="VF240_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177631.1"
                     /translation="MAQSTDVGQPTPVFSSDISGRIAPRDLGDARLTRHFYGFAATEG
                     DLVVTVESTDFNGDVDVFTAGTLRPLLKITLYAVGSTPSRVAQSVYIRRDETLILRVE
                     GRTVIDTDAQYRIRLTGSFKPAAGAPPQAADSQSLEAVETASTTVKGRRTTSTGARIP
                     QPLEETPAQPETAATEPAATEAAREPARRPAGRRSTSRSSTRAGSRRARPPARDTSEN
                     ASPETPKPADAAGAEASKTPEERGATETPAASETPTPRATRTRSPRRGSTRRSAGRSA
                     DTRANREATEAAADPSSAEPPAEPSTRLVIMLKDGELFARDMKAVRRVTVERGQIVVV
                     ETDGKITRHPLASVQRMAIEP"
     gene            16686..17399
                     /locus_tag="VF240_20405"
     CDS             16686..17399
                     /locus_tag="VF240_20405"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7177632.1"
                     /translation="MSKPESIDVQEFPVALAGEKVGAGTGRWRALVVLGIFAVALACY
                     FWSGWWRTPERLTEMGYGVEAAVAIILMMGFAWAFALPASAFLFVTPLLFPPHISTLI
                     TTAGVALGTTFGYAVARWVGGPWVERFRDGRLSRFLSRHSSFIALFGIRIAPGGPHGF
                     VNYAAGLAGIPLFRFVAATTAAMAIKSYVYAQAVHNTVGARSLGDALNARTMLSLTAV
                     AILALAGHVLRERFRSRES"
     gene            17508..19685
                     /gene="bchI"
                     /locus_tag="VF240_20410"
     CDS             17508..19685
                     /gene="bchI"
                     /locus_tag="VF240_20410"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0010007 - magnesium chelatase complex
                     [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HEX7177633.1"
                     /translation="MFIIPDASALDKRVARETMIGHPTSKIQSTQQSARTRVVYPFSA
                     IVGQEEMKLALLLNAIAPTVGGVLVMGERGTAKSTAVRAVADLLPSIKVVRGCAYNCD
                     PQDSETLCGDCRAKLEGAKRLPAERAAAPLVELPLGATEDRVCGTIDVEQVLRDGVRA
                     FEPGLLARANRGFLYVDEVNLLEDHLVDLLLDVSVTGTNKVEREGVSLEHPSRFVLVG
                     SGNPEEGELRPQLLDRFGLFTEVRTVSDVDERVEIVERCERFAADPSGFVEAFGVEQE
                     SLRRRIARARRTFASVKIGRPLLRKVAELCARLGVDGHRGELTIVRAGRALAAFEGRR
                     EVAAEDVQRVAGMALRHRLRRDPMEQTQGGARIEDELEELLDGADAGATESKSSKQLT
                     DDSGGATEQTDRAAGSDSTDEPRGERGGEQGRAPRGKETVIPSEPASLTVDSFTQLPT
                     SSTRAGKGKATPSRRHSLAGKSVYSTRGRYAGATQAKTGAGVLAIDATVRESAVRAVS
                     RREGSSSAAVRVRVGDLRYKRFRSREGTLFIFAVDTSGSMAANRIGQAKGALAHLLRR
                     SYVKRDRVALVCFREEGAEILMRPSAAASRAGRILEALPVGGATPLPAGLRSALDVAL
                     KAVSDGARRVVLVLFTDGRANVPFGGRMAGDRAASRAQILSEVRVIGAALRRLDVTSL
                     VVDTSNRFTSGGEAAELATALGGTHMTLPPGASQQELFEALSV"
     gene            19710..>21325
                     /locus_tag="VF240_20415"
     CDS             19710..>21325
                     /locus_tag="VF240_20415"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX7177634.1"
                     /translation="MRNEERDRRVLELYEQVLEVEQRLIPTGLHVFGLAPDDEQCADM
                     LRMVASFDRPEAGARSLPALVAEGLGLDREVLSGANAEEAEAALIARERVDEIVRESV
                     ARFMRGGKEPAVVYLEAEAHVGAVESRKVFDLLEQVRAQLRVNSELDSLACALRGEYI
                     EPGPGADVVQNPSILPTGRNTHAINPYSVPSDMAYARAERVVKLLLERHRAERGRYPR
                     AMALVLWGLDNIKTQGEGVAQALWLLGVRPVRDAMNRVADVEPIPLAELGRPRIDVVM
                     TVSGIFRDLFGATMLLLDKAVRRVAELDEPAELNYVRANILDESRASGCPFDEAALRI
                     FSNAPGNYGTNVNFMVLDSQWETSDALGELFVTRKCFAYGRDRSGRALEGREAPGAMG
                     RALARVEATYQNVDSFEIGMTDVDHYFEYLGGVSRAVESRAKARPSIYVSDSLSPQVK
                     VRSLEETIRLETRAKTLNPKWYEGMLKHGFRGVAEIESHVANTFGWSATADAVDEWVY
                     AEVAETFVFDEAMLERLSRLNPHSARALVGR"
BASE COUNT         3826 a         6936 c         7038 g         3525 t
ORIGIN      
        1 actggcggca caataccgcg agcggtagcg agcgggtgta gctgtgaaga gggcctgcct
       61 cgcgtgagtc agacccgctc gctaccgctc gcggtactgt gccgctcacc caacttctct
      121 acgcctcaca agccgccggg cctttgcgtc tttgtgtgaa accaataaac tcagtccgtc
      181 aaggctaaca acaatcaaag acgcccatac gatgcacccc aacctccgtt ccttccttca
      241 gacgctcacg cgagaagacg acctcctcac cgtcgaggcg gaagttgacc cctatctcga
      301 actcgcggaa gtccaccgcc gcgtcatcga gcgcggcggc cccgcgctcc tcttccgcaa
      361 ggtgaaaggc agccgctacc ccgtagtcac caacctcttc ggcacggagc ggcgcatcgg
      421 gctggccttc gggccgaagc cggaagcgct ggtacgccgc gccgtcgaga tcgccgaatc
      481 gcttctgccg ccgcgtccga aggagctttg gcgacacaag tcgctcgcac tcgaagcact
      541 gcgcctcggc acgcgtcgcg tccggcgcgc gcccgtcgcc gaagtggttg aacgcccggc
      601 gcgtctcgac gagttgcccg tgctcaccac ctggcaggag gacggcggcc ccttcttcac
      661 gctccccctc gtctacaccg agcaccccga gacgctcaaa cacaacctcg gcatgtaccg
      721 cctgcaacgc ttcgactccg gctccaccgg cctccactgg cagatacaca aaggcggcgg
      781 tttccactac cacgaggccg agcgcgccgg acaggcgctc cccgtcagcg tctttctcgg
      841 cggccctccc gcgctcatcc tcgcggccat cgcgccgctg ccggaagaca tcccggagct
      901 tgtgctcgcc tccgtgctcg cgggcgagcg agtggcggtc gccgcgaatc cgctcggcca
      961 cccgcaccgc ctcgtcgccg aggccgagtt cgctctcgtc ggccgcgtgc cacccgtcga
     1021 gcgcaggccc gaaggcccgt tcggcgatca ctacggctac tactcacttc aacacgacta
     1081 cccggtcttc aacgtcgaag cagtcttcca ccgcaaggac gccatctacc cggcgacagt
     1141 cgtcggcaag ccccggcagg aagatttcta catcggcgat tatctccaac gtcttctctc
     1201 gccgctcttt ccgctcgtca tgccgagcgt gcgcgacctc tggacatacg gcgagaccgg
     1261 cttccactcc ctgtgcgcgg ccgtcgtgcg cgaacgctac ggccgcgagg cgctcgtctc
     1321 cgccttccgc atcctcggtg agggtcagct ctcgctcacc aagtttctca tcctcaccga
     1381 cacgccgcag gacttgcgcg acttcccgcg cctcctcgaa cacgtcctcg cccgcgcacg
     1441 tttcgagcgc gacctcttcg tcttctcaaa tctctcgatg gacacgctcg actacaccag
     1501 cggcaaagtc aacgaaggca gcaaggccgt cctgctcggc ctcggcgatg cggtgcgcga
     1561 gctgccgcgc gagtttcgcg ggcgtctgag cgccggcctt cgacgcgccg aagtcttctg
     1621 cggcggctgc ctcgtggtcg aaggctcatc cttcgccgag gacagggaat tggccgcgcg
     1681 cgtcgcggct gatgcggtgt tcaaagactg gccgctcatc atcattcacg acgacgcgag
     1741 cgtcgcgcac aagtcggccg acttcctctg ggcgacatgg acgcgcttcg agccggcctc
     1801 cgacattcac gccgcggcca cgacggtcga gcgtcaccat ctctcctaca gcccgcccat
     1861 cgtcatcgac gcccgcatga agcccggcta ccccgatgag cttgtagtcc gccccgacgt
     1921 ggcggaactc gtcagccgcc gttgggatga atatttcccg cgcggtctca acgccgaagg
     1981 gagcacttcc aatgtctgaa acgagcgatc ccccccgtgt gatgagcgtc gtcgaggaaa
     2041 cctcctgtgt catcgtcggc ggcgggccgg cgggcgcggt gctctccctg atgctggcgc
     2101 gaaagggcgt ccccgttatg ctccttgaag cgcacgacga ctttgaccgc gacttccgcg
     2161 gcgacaccct ccacccctcc gtgctggagc tgatggacga actcggtctc gccgaccgcc
     2221 tccttcaact tcgacacagc aaactccgca gcgccaacct cgcgacgccc gacggcaccg
     2281 tgaccgtcgc caacttcgga cgcctgcgca cgcgctaccc ctacatcacc atgctcccgc
     2341 aggcgaagtt tctcgaattc ctgaccgacg aggcgaagtg ttatcccgag tttcgtctcg
     2401 tgatgggggc acgcgtggag gagttgatcg aggaagacgg ggccgtgcgc ggcgtgcgtt
     2461 acgagggcga cgacggctgg cacgaggtgc gcgcgccgct caccgtgggc gcggacgggc
     2521 gcagctcgcg cgtccgccga ttgagcggcc tcgacgccga cacgattaag acctctccga
     2581 tgatggacgt gctctggttt cgcatcccgc gtcggccgga cgacccggag ggcctgctcg
     2641 gccgtttcgg gcgcggccac ggcgtcgtca tgttcgaccg cgacgaacag tggcaggtgg
     2701 gcttcatcat catcaagggc agcttcaagg aggtgcgcgc ggcgggcatc gatgcgctga
     2761 aagagtcgtt cgccgagacc gcgccagagt tcgccgaccg caccgaacat ctcacggagt
     2821 ggaaacagtt cgcgatgctc tcggtcgagt cgagccggct gaagaattgg acgcggcccg
     2881 gactgctcct catcggcgac gcggcgcacg tcatgtcgcc cgtcggcggc gtcggcatca
     2941 actacgccat tcaggatgcg gtggtcgcgg caaacgtcct cacggagcca ctcctacgcg
     3001 gcgacgtgag ggatgaattc ctgcgagaag tgcagcgcca gcgcgagtgg ccgacacgcg
     3061 tcattcaggc gatacaggcg gggggccaac gggccgtcgt cgccaacgcc ctcaagtccg
     3121 accaaccatt ccgcgtgccg cttctcgcgc ggctacttct cagaatccca ctgctccgcg
     3181 acatccccgc gcgcatcatc gccttcggcg tccgacgcgt ccacgcgaaa atttaagttg
     3241 acagtgggag gctgatgcgg aagacagcgc ctccgcgcgc gtcgttgcgc gccgtgagtg
     3301 tgccgccgtg gtcggtgacg acgcggtgtg cgagcgcgag gccgatgccg tgccccttcg
     3361 acttcgtggt gaagaagggg atgaagatgc gtcgcaggtc ggcctcggga atgccgccgc
     3421 ccgtatcagc gacctcgacg acggcgaact cgcgcccgcc ttctccgcgc gcccgcgacc
     3481 cgctcacgac gacccgccgc tccgtcgcct cgtcgccgac agactcggcc gcgttacgca
     3541 tgacgttgag aagcgcctgc ctcagcaggc gctcgtcagc ccgcacgacg gggaattcac
     3601 cttccacgcg cagctcgaca cgcagctcca agtaaagctt ctccacctcc gcccggcagc
     3661 tctcgacgag ttcggcgagg gacacgtctg ccgggtcgag cggcttcggg cgcgcgaagt
     3721 tgaggaagga agtgaccatc tccgaaagcc ctcggacttc gtgcagcagt gcggcggcgg
     3781 cggcccggcc gtgttcgtcg atgtcgagcg tctgaaagag ctgcgcgtat ccgtggagcg
     3841 tggcgagcgc gtttttaaat tcgtgggcca gcccggcgga catctcgccc agactctcca
     3901 gattccgttt gagcgccacg gcctcgcgga gttgcaagac ctcggtgatg tccgtgagca
     3961 ggcagagcgc gccgcgcccc tcgccgagtt cgatgggggc gaccgtcacg ccgagacggc
     4021 gcaccgcgcc cccttctgtc cgcgccgcca cctcttcgcg ccggaagacc tcgcccgtca
     4081 gcaggcagct ctcgatcagg tctgacatct ccggcgcgtt gcccagcagc tcgacgtagg
     4141 cgcgcgcttc cgtcccgccc acaatcccga gcaggacgcg cgcgggcgcg ttgacgagcg
     4201 tggcccggcc atttgaatcg actgccagca ggcccgacgg gacgctcgcc acgatgcgct
     4261 cgctgaactg ttcggcggag acggcgcggg cgctggcctg cgcgctcaac gtctccaact
     4321 cccgctgctt ggagcggagc tgcgcgacaa cctcgtgaaa tgtgttgagg acgaactgcg
     4381 tctcgtcctg ctcgccgacc tgtcgccgcg cctcgacggg cgctcgctcg gccgcgtcca
     4441 cgagttgccg gtaaggcttc aacagccagc gcggcagcag gagcagcgac gggacgctga
     4501 cgatcagcgt gacgacgaag acggtgaaga cgaccgaagc gcgggccgga cgcgccgccg
     4561 gtgtcagcac gacgagcgtc aacgtgaaca gattggccaa cagcagcagg ccgatgacca
     4621 gggccagcac aagctgcacg cggttctcgt aacggggcat aagaggagtg ataagtgatg
     4681 agtgataagt gataagagct tttagctaat aactaatggc tctttcttat cacttatcac
     4741 tcatcactta tcacttaatc acctgaattg cgacgcgtac caatcagcga tctgggtgcc
     4801 gaagaggaga ctcacgaggg cgccggcgcc gaggaagatg ccgaagggca gaagcatctg
     4861 cgtgtcgcgc tcgccgcggc gcaccatgag ggcgacgccg gcgagcgagc cgccgagcac
     4921 cccgatgaag atcgtcagca gggtgagcgg ccagccgaga tatgcgccga ccatgaacat
     4981 catcttcacg tcgccgaggc ccatcgcctc gacgccgcgc gcgcgctccc agagccatcc
     5041 cgcgagccac aggaagccgc cgcccacgag tgcgcccagc agtgcgccga cgagcgagac
     5101 gagccatacg ggccagccgg ccaacgatcc gcgcgtcaga aattcgacgc cgtaaaggtt
     5161 cggaacgacc acacgcgcca cgagcgccag cgcgaggccg gggtaggtga tgacgttggg
     5221 caggagcatg tgctcggcgt cgatgaagat gagcgcgacg agggcggcga cgaagacgag
     5281 gtcgaagggg agcgagagcg tcaggcccga acggaaccag aaggtgagtc cgaagagggc
     5341 ggccgtgaga aactcgacgg ccggataacg cgcggagatg tgcgtgcggc agtcgcggca
     5401 gcggccgcgg agcgcgagcc aactgaggac gggcacgttg tcgtaaaagc gaatcgacgc
     5461 gccgcacgag gggcagcgcg agtttgggaa gacgatagac tcttcgcgcg ggacgcggtg
     5521 gatgaccacg ttgaggaagc tgccgatgag gccgcccagc acggcgacga acgcgagtgc
     5581 gagggcgggc ggcagcccga gaaaatcgtg agacgggaga aggctcagga agttgggcat
     5641 gagaggcggc tcgtcgtcgt cagtttagag acgctgtggg gcaaagtcgg cttgacgcac
     5701 gccgaacccc ttccgcatat taaccggacg aagctgtttg cgaaagacag ttgtggaaca
     5761 aacgtgcctg aaggggggaa tgtcggttga actctcgcaa gccgatcttt gcgctgctgg
     5821 ccgtcggtat cgccgtcgtc atcgcgctca cctttgtccc atacgccttc ctggagaggg
     5881 ctggccgcgc cgtcaccgcc atcctccccg gcgcgcgcga agcgacggtg gacgcccctg
     5941 cccggccgtt gctgccggcc ttcaacgtgg aaggctggta cgcggagcgc ggcgaagacc
     6001 ccgcccgaca cggcgtccac atcgagagca tcgacggcaa gagcgtcctc gcctcacaca
     6061 accccgacct ccccttcaac cccgcctccc tcctcaagct cgcgacttcg ctcgccgtgc
     6121 tccgcgcctt cggccccgat caccgctttg agacgcgcgt ctacaccgac ggcgtcgtcg
     6181 gccccgataa gacgcttaaa ggaaatctcg tactcgcggg caacgacccc atgttcggcg
     6241 acgtggcggc cagtctcgtc gcacgcgagc tgcgcgcgcg cggtgttgaa aaggtgagcg
     6301 gcgaactgct cgtcacgccc ggcttctcct tcaactacgc cgagaaggcc gatgactcgg
     6361 ccgagcgcgt cgcgaaggta atggagatga aagagaaggc gacaggcgtg ggcgagacgc
     6421 cggcgggcga gccgcgcttc gtcgtcaagt cgtacccgct acgcgaaata cttctttaca
     6481 tgaacgccca ctcgaacaac ttcgtcgccg agcggctggg cgcgttggtc ggcggccccg
     6541 aggggctgaa gcgttttctg gtcgaagaat tgaagctgcc ggccgagcag gtggaagtgg
     6601 agcgcacgtc ggggcgggag aataatcgca tgacggcgcg agggatcgtc tcggtcgtcc
     6661 gcgcgctcaa ggccgaggcc gagcggcacg gcatgcgcgt cgaagacctc atgccggtgg
     6721 cgagcgacga cgcgggcacg ctccgccgcc ggctcgaagg cacgccgctc gaaggcgcaa
     6781 ccatcggcaa gaccggcacg ctcaccgctg aagttgacgg cgggatggcg agcctcgccg
     6841 gcgtcgtctt cacggagacg gccggcatgg tcgccttcgc catcctcgat caggggagcc
     6901 gcatcgccga gaaccgcgaa ctcgaagaca tcctgctcgc gcaggtcatc gcctcacagg
     6961 acatcccgcg cctcatccat agcgaagagg tgcgccgccg actgctcccc gccacgagcc
     7021 tccgcgtcga gccggaagcc ctcgccggcc tcgttgatga gcgcgacgat aagaaacaaa
     7081 cggacgagag ggaatccgac gaggaagaag catccacttc aaaatcaaac gcgaagacgc
     7141 gcgatggtaa acgcgtggag gagaaggcgt cgcagcggtc tcgaaagcct gagccgtcaa
     7201 gggccgactc gcggaagcgg gaagtacagc ggggcgggcg ggcgagcgtg cgggggcgag
     7261 ggcgcaggtg agacgaaagg ctctgggtgg ctcctgcttg agtgttcggc gactcggttc
     7321 accgcggagg cgcagaggac gcagaggggg cgccgcaggc ttgaagcatt cactcaacga
     7381 tgagtgtagg catcaacctc tctgcgtgaa ctctgcgtcc tctgcgcctc cgcggtgagc
     7441 taacttgcca aaaactcaag taggagccag cactaacttt catctgacct atcagcctcc
     7501 ttgttttgca tctcccacaa cagcaggtgc ttgaggaagg cgtggtgcgc cgcgaagcgc
     7561 gcgatggcga agccggcgag gccgtcgcga aagccgccct tgaggacgta gccgcgcgcg
     7621 aaggtcgtcg ggccggcgag cgcaaccttc agaggcgagg ttcgtcgccc ggcttcgtac
     7681 atctgcctcg cggccagcgg cgcgtaacgc tcgccgatca tccggtgatg gtgcaacgcg
     7741 tcgcggacgc tgtagtggag caggtcgccc gcgagcgtct cgaccctcgc cccttcagac
     7801 atcttcacag attcgtggac atatgcaccc gcccagcgtc cacgcccctt cctgtacagg
     7861 cgtagctgat ggtcgggata ccacccgccg ccgcgaatcc agcgccccat gtagaaggag
     7921 cggcgcgcga tgcggtagcc gtcggccagc ccagcctccg gcgcatggag caagtcgtca
     7981 atcgaagcgc gcaactcgtc cgacacgcgc tcgtcggcgt cgaggctgaa gacccattcg
     8041 ccacgcgcct cctcggcggc gaactgcttc tgcgccgcga accccggcca cgaacgcgtg
     8101 atgacgcggg cgccgcagcc gcgcgccaaa tcggcggtcg cgtccgtcga ttcggaatcg
     8161 acgacgagaa tttcgtccgc ccacgacacg gactcgcagg cggcgcggat attctccgcc
     8221 tcgttgaagg tgatgatggt ggccgtgact ttcaaaatgt tcgccggcag aggatacgaa
     8281 cccgtgcggg cgcgcgtcaa tcccgcccgt cgcccaaaac aaagcggccg tggagaggct
     8341 caccctccac ggccgctgat tcatattccg cgctcggtct taacggccga tgggcgaatc
     8401 gacgatcacg ttgttggcgc gcagcgtggt cgaggtcgcg cgctggagtt cggcgagggc
     8461 tttgttgtag tcggtctcgg cccgcagctc ctggttgcgc gcgttgacga gtgagttttc
     8521 gcgctggaag aggaggaagg tcgtcgagcg gccgacctga tagaggcggc gctcgccggc
     8581 gagttgaagc tcggcgtttt cgcgcgccga gcgggccgcg agcacgcgcc ggcgcgcggt
     8641 ctcgacagac tgcacggcgt tgcgtacttc gacctcgacg ccctgttcct gtagccgcgt
     8701 gttggcggca agctgctcgc gctggatgcg taccgtggcg aggttggcct cggccgtgcg
     8761 gttacgcagc gggatttgga tcgtcacgcc gacgacgatg ttgcgcgtgc tgaggctaaa
     8821 gaggtttttg agcgtctgga agtagccgcc ctgaaactgc gagggcacac ccgcgccacc
     8881 ctgctcaacc tgcggcgtcg tgagggggtc gagacccagg tcgcggcgcg tgcggttgat
     8941 tgactggaag aggaaggcgt cggcgttggt ggccgcgacg gcgtcgccgc cgacgagatc
     9001 gacgatgcgc gtgccgctgc cggcgctcgc gctcggcgtg ccggcgagtc cggtggtgga
     9061 gacggtgccg gtcaggtcga tctgcggctt ggtctggttc ctgaagaact ggatgtcgat
     9121 gtcgttgatg tcctgctgga gcttgagccg gcgtagctcg gggcggttgt cgcgcgcctc
     9181 tttgatggcc gtctccagac tgacgggcgt gtcgtcgaag cgcgcctgat cggtcggcac
     9241 gatcacgctc gaccactcgg ccgaaagcgg ctccttgatg atcaggctct tcaggttgtt
     9301 ctcggcgatg gtcacgctct gcgacgagag cagcagggcc gattcgcggt tcgatagctc
     9361 ggtctgaatc tcggcgcgct ccagcggggc cgtggcgccg gcggcgacgc tggcctcggt
     9421 gcgccggaag ttttcgcgcg cgaggttgag gttggcgatc tggttctgct cgtcgcgcag
     9481 ggcgaagacg aggtcccagt aggcgcgctg cacctgcgag atgatttcga tggtgcggcg
     9541 gcggaaatcg gcgtcggact gctcaaggcg tttctgttgg atgcgaatct gctggcggtt
     9601 gcggtcgatg gagcggttgc gccagagcgg ttgcgtgaac tgaaggccct gactcgacga
     9661 gtagaacggg ttgagggtgt tgttgctggc ggtcgtcgtc tcgcgcgtgt tgttgaagaa
     9721 gtaggtgtag ttgccgccgc ccttaccgaa ctgccgggtg acggagaagt cgttgttgag
     9781 gtcggtggtc gcgacggtgc cggcggcgtc ggccccgccg aagatgttga ccacgggccg
     9841 caccgagttg ttgatctctg gacggaaggt gagcacgggg tcgtagacgc cgcgcaggga
     9901 gagcagttgc gactctacca agcgcacgtc ggtgcgggcc acctcgatct cgttgttgtt
     9961 ctccagcgcc cgccggatgg cttcgttgag cgtgagcggg atgttgtcgc cgctgaccac
    10021 gccgacgcgc acgagcgagg gcatcggggg gaggggacga ggctgcgtca gggtcagaag
    10081 ttgctcggtg ccctgctcgg cggaaggcac cgtctcgggc agcggcgtcc cggtcacggt
    10141 cggggcgtcc gtggggcgct cgacgttggg ggtggtaacg ggcgtctgcg gggcgcgctc
    10201 cgtgccgggg ggcgtttgcg gcgcgggccg ctgtgccccc ggcggggtcg tcggattggt
    10261 cggactcggg ctgatgtcgc gggggacggg ctgcctctgc tccgtgccgg gcgggcgcgt
    10321 cgcgtcgcgc tgtgccgggc tggtggggcc gggggcgggc gtggactgcg gcacggcctc
    10381 ggcgccggcc acggcgagcg caaactgctt gcgctggaag gtcagctcca cttgattggc
    10441 gccgggctga aggtcaacga aggcgactgc ggtctcgtcg ccggcgcggg ccatgagcgt
    10501 cccggagccg gcccggaggt cggggaagga gaatcggccg gcgtcgtccg tctgagttct
    10561 cacatcgttc gtggcggtgc gtcggaaata gaccgccgcg ctggcaaccg cctcgccggc
    10621 gatgttgcga accgtccccg tcagcgtgct cgccgcgtca gacgatgcgc tcggccgcgc
    10681 gttgtctgaa gagagcgcgt ggaggggggc cggcgccatg acggcgaagg cgagaatgag
    10741 agccgcgacg tgtctcgacg cggggcgcgc gcacaaatgt cgcaagaagc gtaaaccttt
    10801 actcagcatc aaacaaaaac tcctctggat gttgggaacc gcgaccggcc gccggatgaa
    10861 tagccgctta aaatcgaaaa ccgcgggtca gagcctacta cgtcagcgcc gcatacacgg
    10921 tttcataaac atacacaaac catgccacgc ataagcactc gattgcgtgg atattccccg
    10981 ccaaaaggcg acgaatgagt tggatgcgtg ccggcctcga ggggttctcg tcccccgagc
    11041 gaacaacgca ttcccgcgaa tgggactgga gagcgtgcgc cggggcggcc ttcgggcgag
    11101 tggctggctt gacaccccgt cgcggcgggc attatcttgc cttttcttta accgcaacac
    11161 gctgagcgcg cccgtcttcc gaacacctcg gccgagggcc gaagctacgg ccgccgcccg
    11221 cggcccgcgc tgacaatgag ccgggccggt atctttatcc cgtcattgga aatgatttcg
    11281 aggaggccaa aaacagatgg cagttaaagt cggcatcaac ggtttcgggc gcatcgggcg
    11341 caacatcctc cgcacaagct tgggcgactc cgagatcgac ttcgtcgccg tcaacgacat
    11401 cactgacacg aagacgctcg cgcacctgct caagtacgac tccgtactcg gcaacctcga
    11461 acacgagatc gccgcgaccg aaaagggcat ccgcgtcggc tccgacgagt tcacggtctt
    11521 ctccgagcgc gacccggcga agattgattg ggagtcggtc ggggccgaga tcgtcgtcga
    11581 gtcaacgggt ctgttcacca acgccgagga cgcgcgcaag catctgcgcg gctccgtcaa
    11641 gaaggtgatc atctccgcgc cggccaagaa cgaagacatc accatcgtgc tcggcgtcaa
    11701 cgaaggctcc tacgacccgg ccgcgcacaa catcatctcg aacgcctcct gcacgaccaa
    11761 ctgtctggct cccgtcgcca aagttatcca cgagaaattc ggcgtgcgct ccgcgcagat
    11821 gacgaccatc cattcgtaca cgaacgacca gcagctcctc gacctgcccc acaaggattt
    11881 gcgacgcgcc cgcgccgccg cgctctccat gatccccacc tcgacgggcg cggccaaggc
    11941 cgtcgcgctc gtgctccccg aactcaaggg caagttcgac ggcatctcgg tgcgcgtgcc
    12001 cacgccgaac gtttcactca tcgacgtggt catccgggtg gagaagcaga cgaccaccga
    12061 ggaggtcaac aaggcgctca aggacgcggc caacgaggag ctgcgcggcg tcctcgcctt
    12121 ctccgaggag ccgctcgtct cggccgactt ccgcaagaac cccaactcgt ccatcgtcga
    12181 tgccgagtac accaaagtca tcgacggcga cctcgtcaaa atcctctcgt ggtacgacaa
    12241 cgagtggggc tactcctgcc gcgtccgcga cctcatcaag tacatcgcgg ggaaaggact
    12301 ttaagagccg tgataagtga tgagtgataa gtgataagac aaaaacctgt cttactcttt
    12361 cttatcactc atcacttacc acttatcact atgccaaagc tttccatcaa agacctcgac
    12421 ctgaagggca agcgcgtcct tatccgcgtg gacttcaacg tgccgatcaa ggacgggaag
    12481 gtggaggacg acacgcgcat cgaggcggcg ctgccgacga tcaggcatgc gaccagtgcc
    12541 ggcgcgcgtg tgattctcgc ttctcatctg ggtcggccga agggcgagcg cgtggagaag
    12601 tacagcctgc gtcccatcgc cgagcacctg tcgggcctgc tgggcgcgct ggtggccttc
    12661 gcggacgact gcgtcggtga ggaggcccgc gcgaagatcg agtcattgca gaacggcgac
    12721 gtgctcctac tcgaaaacct gcgcttccac gccggggagg agaagaacga cgaaaacttc
    12781 gcgcgcgagc tagcgagcct ctgcgacttg tacgtcaacg acgccttcgg cgctgcgcac
    12841 cgcgcgcacg cctccacggc cggcatcacg aaggttgtct cgcaaagcgc cgccggcctg
    12901 ttgatggaga aagagctgac ttacctcggt cgcgtcatcc acgaccccgc gcatccgttc
    12961 gtcgccatcc tcggcggcgc gaaggtctcg gacaagattc ccgtcatcga cgcgctcatc
    13021 acacgcaaag ttgacaagct cctcatcggt ggggcgatgg cctacacctt cttcaaggcc
    13081 gacgggttta cgatagggaa gtcgctcgtc gaaaacgaaa tgctcgatac ggcgcgcgag
    13141 ataaaaaaga aggctgaaga tgccggggtc gagctgctgc tcccgaccga ccatcaggtc
    13201 gtcgatagct acgagccggt caagggcgag caggccgtca gcaagaccat ccccatcgag
    13261 ttcaccaacg ccggacacgt cggcttagac atcggcgtcg agacggtcgc gcacttctcc
    13321 gcggcgctca aggacgcgaa gaccatcatc tggaacgggc cgatgggcta cttcgaggag
    13381 aagcctttcg atcagggcac tatcgccatc gcgcaggccg tggccgaagc ggccgacgga
    13441 ggcgcaacgg tcatcgtcgg cggcggcgac tccgtttcgg ccgtcacccg cgccggcgtc
    13501 gccgaacgca tcacgcacat ctccaccggc ggcggcgcga ctctcgaatt catcgccggc
    13561 gaagaactgc ccggcgtcac ggcacttaac gacaaatgaa gtcaggagtc agaagtcagg
    13621 agtcagaagc aggaataata gaagaggctc gccgtatgtc ttcattctga cttctgactt
    13681 ctgactcctg actccctaat gttttatgcg taaacctgtc atcgccggaa actggaagat
    13741 gtacaagctg gtcgccgact cggtggcgac ggcgcagacg ctgaagtcgc tggtctcgaa
    13801 cgcgaaccac tgcgaggtcg tcatcgcgcc ggtgtttacg gcgttgaaga cggtcgccga
    13861 caggctcgaa gggtcgaaca tccgcatcgc cgcccagaac tgcgcggccg agtcgaagca
    13921 cggggcgcac acgggcgagg tggccgccga catgctgcgc gacgccgggg cgtcgcacgt
    13981 catcgtcggc cactccgagc gccggcacat ctacggcgag acggacgact ttgtagggcg
    14041 caaggtcggg gccgggctgg cggcgggttt gatcgtcatt ttgtgtgtgg gagagacgct
    14101 tgaggaacgc gagggcgctc aggcggaggc ggttgtgagg agacatctgg agacgggctt
    14161 gagcggtttg acagcctcag acctcgaccg tatcattgtc gcctacgaac ccgtctgggc
    14221 catcggcacc gggcacaccg cgacgccgga gcaagcgcag gaaatgcacg cgtttgcccg
    14281 gcgcgtttta gcggagaagt tcggcgaaga agtggccgcc cgcgttagaa tcctctacgg
    14341 cggatcggtc aagccggaca acatttcgaa gctgatgctt cagccggacg tggacggcgc
    14401 gctcgtcggc ggcgcgagtc tcgaagccga aagcttcgcg cgtatcgtca actataactc
    14461 ttgatttaag gaaaattatt ttgctgattt acgtcctcta cggaattttc atcctcgcct
    14521 gtctcgcgct catcctctcc gtgctgctcc agccggggaa ggccgacgcc ggctccctct
    14581 tcaccagctc gttttcgagc acagccttcg gcccgcgtgg cacgcagacg gttctcgcca
    14641 agatcagcat cgtttcggcc atcaccttca tgcttgtcgc gctactgatt tcgatgggcg
    14701 tgggcggtta ccgctccgtc atcggttcgg gcgacacggc gacgcccgtg tctgaggcca
    14761 cacccgcacc ggccgcggag acgcccgctc aggaaggcgc agcccagccg aacacaaacg
    14821 ccgcccccga cgcacccgcg gcaggcgatg tgccgccggc cgccgccacg ccggccccgg
    14881 ccgcgtcgcc cgccccgagc agctagcacg tgtgctggca gggccgaacg cctttttgtt
    14941 aatcttgaag tgttagcttg acagcacggg ccgaagtggc ggaactggta gacgcgcacg
    15001 tttgaggggc gtgtgaggta actcgtgggg gttcgagtcc ccccttcggc attcgcggaa
    15061 caacagggcc tgacgcgtcg tgcgtaaacg acgtgtcagg ctttttcaca tttcaacggc
    15121 ggccgcgcct gtatcgaaca ggggaagaat gcaggccaga gggaggtgtg aaatctcaac
    15181 ggtcttggca gtgttcagtt gagtatgtgg cttcaaagac tcaccgcgga ggcgcagagg
    15241 acgcagagga agcgcagagg gctgacgagt tcactccacg tcgagtgtat cggtcaagcc
    15301 tctgcgtatt ctctgcgtcc tctgcgcctc cgcggtgagt ctacttgcct tacactcaag
    15361 tgaacgtcgc tcaaaacgtc gagaccagcc catccgttct gaccctggac ttaccctggc
    15421 cctccaattc ggaactcgat tccatcagga gacgactccg cggggtcgtc gcagcacgcc
    15481 ctagtggcgg tctgtttttc ctgctaaact ctccacctca aagctggagt ttaccgtcgc
    15541 atgagtctgc ctttaagaac agccggggta aaattcatcc gcggagtcgt gtgcgcgtta
    15601 tcactcgcgt gcatagctgt ggcgcagtca acggacgtgg ggcagccgac ccctgtcttc
    15661 tccagcgaca tctccggccg catcgcgccg cgcgacctcg gcgacgcgcg tttgacgaga
    15721 cacttctacg gcttcgcggc gacggagggc gacctcgtcg tgacggtcga aagcaccgac
    15781 ttcaacggcg acgtggacgt gttcaccgcc ggcaccctcc gcccgctcct caaaatcaca
    15841 ctctacgccg tcggctcgac tccgtcgcgc gtcgcccaga gcgtctacat ccgcagggat
    15901 gagacgctca tcctgcgcgt ggaaggacgc acggtcatcg acacggacgc gcagtatcgg
    15961 atacgcctca caggctcctt caagcccgcc gcgggagcgc cgccgcaagc ggccgactca
    16021 cagtcgcttg aagcggttga gaccgcctcg acgaccgtga agggaagacg caccacttcg
    16081 accggcgcgc gcattcccca gccgttggag gagactcccg cgcagcctga gacggccgcg
    16141 acggagcctg ccgccactga agccgcgaga gagccggcaa gaaggccggc gggacgccga
    16201 tcaacttcga ggtcttcgac gcgcgccggc tctcgacgcg cgagaccgcc ggcccgagac
    16261 acttccgaaa acgcttcgcc ggagacgccg aagccggcag acgcggcggg cgcggaagcg
    16321 agtaaaacac cggaagagag gggcgcaacg gaaacgccgg cggcgtctga aactccaaca
    16381 ccgagggcga cgcgcacgag gtcgccgcga cgcggcagca cgcgcagaag cgccggccgt
    16441 agtgcggata caagagcgaa cagggaagcg acagaggccg cggccgaccc ttcgtccgcc
    16501 gagcctccgg cggaaccgag cactcgcctc gtcatcatgt tgaaggacgg agagctgttc
    16561 gcacgcgaca tgaaggcggt gcggcgtgtg acggtcgagc ggggccaaat cgtcgtcgtc
    16621 gagacggatg ggaagatcac ccgccaccca cttgcgagcg tgcagcggat ggccatcgag
    16681 ccgtagtgtc gaagccggaa tcaatcgacg tgcaggaatt ccccgtggcg ttggccggcg
    16741 aaaaagtcgg cgcgggcacg ggtcgttggc gcgccctcgt cgtgttgggc atcttcgccg
    16801 tcgccctcgc gtgctatttc tggagcgggt ggtggcggac gcccgagcgt ctgacggaga
    16861 tgggctacgg cgtggaggcc gccgtcgcaa tcatcctgat gatgggcttc gcgtgggcct
    16921 tcgcgcttcc ggcctccgct ttcctctttg tcacgccgct gttgttcccg ccgcacatct
    16981 cgaccctgat aacgacggcc ggcgtcgcgc tcggcacgac cttcggttac gcggtcgcgc
    17041 gctgggtcgg cggcccgtgg gtcgaacgct tccgcgacgg gcgactctcg cgattcctct
    17101 cgcgccattc cagcttcatc gcgctcttcg gcatccgcat cgcgcccggc ggcccgcacg
    17161 gcttcgtcaa ctacgccgcc ggcctcgccg gcatacccct gttccgcttc gtcgccgcga
    17221 ccacggccgc gatggccatc aagtcttacg tctacgcgca ggccgtccac aacaccgtcg
    17281 gcgcacgctc gctcggcgac gcgctcaacg cgcggacgat gctctccctc accgccgtgg
    17341 ccatccttgc gctggcgggc cacgtcctgc gcgagcgctt caggagccgt gaatcgtaaa
    17401 tcgtcaatcg ttaatcgtgg ttgagtcagg atcaactcgg gtggcgaggc gctagtcgca
    17461 cgacgcgcaa tcgattgacg attgacgatt tacgattcac gattcacgtg tttataatcc
    17521 ctgacgcttc ggccctcgac aaaagggtcg cgcgcgagac catgatcggg catcccacaa
    17581 gcaaaattca gagcacacaa cagtccgcac gcacgcgcgt ggtctatccg ttctcggcca
    17641 tcgtcgggca ggaggagatg aagctggcgc tgctcctaaa cgccatcgcc ccgacggttg
    17701 gcggcgtgct cgtcatgggc gagcgcggca ccgccaagtc aacggccgtg cgcgccgtcg
    17761 ccgatctgct gccttcgatc aaggtcgtcc gaggttgtgc gtacaactgc gacccacagg
    17821 actccgagac gctgtgcggc gactgccgcg caaaactcga aggggcgaaa cggctccccg
    17881 ccgagcgtgc ggcggcgccg ctcgtggagc ttccgttagg cgcgaccgaa gaccgcgtgt
    17941 gcgggacgat tgacgtggag caggtgcttc gcgacggcgt gcgcgccttc gagccgggac
    18001 tgctggcgcg cgccaaccgc ggcttcctct acgtcgatga ggtgaacctg ctcgaagatc
    18061 atctggtgga cttgctcctc gacgtgtcgg tcacgggcac gaacaaggtc gagcgcgaag
    18121 gcgtttcgct cgaacacccg tcgcgcttcg tcctcgtcgg ctccggcaac ccggaggaag
    18181 gcgagctgag accgcagtta ctcgaccgct ttggcctctt cacggaggtg cgaacggtct
    18241 cggacgttga cgagcgcgtc gagatcgtcg agcgttgcga gcgtttcgcc gccgacccgt
    18301 ccggcttcgt cgaggctttc ggcgtggagc aggaatcgct gcgccgccgt atcgcgcgcg
    18361 cgcgtcggac gttcgcatca gtcaaaatcg gccgaccact gctgcgaaag gtggccgaac
    18421 tctgcgcgcg gctcggggtg gacgggcacc gcggcgagtt gaccatcgtc cgcgcggggc
    18481 gcgcgctcgc ggcgtttgaa ggccgaagag aggttgcggc ggaggacgtg caacgagtcg
    18541 cgggcatggc cctgcgtcac cgcctgcgcc gcgacccgat ggagcagacg cagggcggcg
    18601 cgcgaatcga agacgaactc gaagaactgc tcgacggggc cgacgccggc gcgacggaga
    18661 gtaaatcgtc aaaacagttg acggatgatt cgggaggggc aacggaacaa accgaccggg
    18721 cggcgggaag tgattcaacg gatgagccgc gcggcgagcg cggcggcgaa cagggcagag
    18781 cgccacgcgg aaaagagaca gtgataccgt ccgagccggc gtcattgacc gttgatagct
    18841 tcacacagtt gccaacgtct tcgacgcgcg cgggcaaggg taaagcaaca ccgtcgcggc
    18901 ggcacagtct cgccggtaaa tcagtttaca gcacgcgcgg acgatacgcg ggcgcgactc
    18961 aggcgaagac cggcgcgggc gtgttggcga ttgacgcgac cgtgagagag agcgccgtcc
    19021 gggccgtgag ccgtagagag ggaagcagca gcgcagcggt tcgcgtccgc gtcggcgacc
    19081 tgagatataa acgtttcagg agtcgcgagg ggacgctctt catcttcgcc gtggacactt
    19141 cagggagcat ggccgccaac cgcatcgggc aggcgaaggg cgcgttggcg cacctcctgc
    19201 gaaggtctta tgtgaagcgt gaccgcgtcg cgctcgtgtg cttccgcgag gagggcgcag
    19261 agatactaat gcggccgtcg gccgccgcgt cgcgcgccgg aaggatactc gaagcattgc
    19321 ccgtcggtgg tgcaacgcct ctgccggccg gtctgcggag cgcgctggat gtggccctca
    19381 aggccgtctc cgatggcgca cgccgcgtcg tgctcgtcct cttcacggac gggcgcgcga
    19441 acgtcccgtt cggaggtcgc atggccggcg accgcgcggc ctcgcgcgcg caaattctgt
    19501 ctgaggtgag agtgatcggc gcggcgctga ggcgactcga tgtcacttct ctcgtcgttg
    19561 acacatcgaa tcgattcacc tcgggcggcg aggctgctga gctggcgacg gccctcggcg
    19621 gtactcacat gacgcttccg ccgggcgctt cgcagcaaga actgttcgag gcattgagcg
    19681 tgtaaatgca agacgagtct gtcacgaggt tgaggaacga agaacgcgac cggcgcgtgc
    19741 tcgaactcta cgagcaggtg ctggaggtcg agcagcgtct gataccgacg gggcttcacg
    19801 tcttcggtct cgcgccggac gacgagcagt gcgccgacat gctccgcatg gtcgcgtcgt
    19861 tcgacagacc ggaggccggg gcgcgctcac tgccggccct tgtcgcggaa ggattgggcc
    19921 tcgaccgtga ggtgttaagc ggcgcgaatg ccgaagaggc ggaggccgca ctcatcgcgc
    19981 gcgagcgggt ggacgagatt gtgcgtgagt cggtcgcccg cttcatgcgc gggggaaaag
    20041 aaccggcggt cgtttatctc gaagccgagg cgcacgtcgg cgctgtggag tcgcgaaagg
    20101 ttttcgacct gctggagcag gtgcgcgcac agctcagggt caattcagag ttggacagct
    20161 tggcctgcgc cctgcgcggc gagtacatcg agccgggtcc cggagccgac gtggtgcaga
    20221 atccatccat cctcccgacg ggacgaaaca cacacgccat caatccctat tccgtcccct
    20281 ccgacatggc ctacgcgcgc gccgagcgcg tcgtcaaact cctgctcgaa cgacaccgcg
    20341 ccgagcgcgg ccgatacccg cgcgcgatgg ccctcgtgct gtggggtctc gacaacatca
    20401 agacgcaggg cgagggcgtc gcgcaggcgc tctggctcct cggcgtgcgc cccgtgcgcg
    20461 acgcgatgaa ccgtgtggcc gacgtggagc cgataccgct cgccgaactc ggccgtccgc
    20521 gcatcgacgt ggtgatgact gtctccggca tcttccgcga cctgttcggc gcgacgatgc
    20581 tgctgctcga caaggccgtc cggcgcgtgg cggaactcga cgagccggcg gaactgaact
    20641 acgtccgcgc caacatcctc gacgagtcgc gcgcttcggg ctgtccgttt gacgaagccg
    20701 cgctccgcat cttctcgaac gcccccggca actacggcac gaacgtcaac tttatggttc
    20761 tcgactcgca gtgggaaacg agcgacgccc tcggcgagtt gttcgtcacg cgcaagtgtt
    20821 tcgcctacgg gcgcgaccgt tcggggcgcg cactcgaagg ccgcgaagcg cccggcgcga
    20881 tggggcgggc gctggcgcgc gtggaggcga cctatcagaa cgtggactct ttcgagatcg
    20941 ggatgacgga cgttgaccac tatttcgagt acctcggcgg cgtgtcgcgc gccgtcgaga
    21001 gtcgggccaa agcgcgcccg agcatctacg tctccgactc gctctcgcca caggtcaaag
    21061 tgcgctcgct tgaagagacc atccggctgg agacgcgcgc caagaccttg aacccgaagt
    21121 ggtacgaggg gatgctcaag cacggcttcc gcggcgtcgc cgaaatcgag agccacgtcg
    21181 ccaacacctt cggctggagc gcgacggccg acgccgtcga tgaatgggtg tacgcggaag
    21241 tggccgagac gttcgtcttc gacgaggcga tgctggagcg cctgagccgg ctcaacccgc
    21301 actccgcacg cgcgctcgtc gggcg
//
LOCUS       DASVES010000489        23179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1061780, whole genome shotgun sequence.
ACCESSION   DASVES010000489 DASVES010000000
VERSION     DASVES010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23179
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061780"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VF240_20420"
     CDS             complement(<1..531)
                     /locus_tag="VF240_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177635.1"
                     /translation="MTHATEEMAPDVAPELSPEAAGARPAAAAGGSTLIEFPGARSIP
                     QWRKDLSERVREIQKRKAREAAPDVDETASLPAEEPSGVSAADAAPSEEGSAASRLGL
                     VPPLPDAPELNPLVAAALKRIERARQQPAARRSGPSGGRAAATAVARVAEEQYEPRVR
                     PQVEARGETQQSPAVEV"
     gene            complement(846..1715)
                     /gene="lepB"
                     /locus_tag="VF240_20425"
     CDS             complement(846..1715)
                     /gene="lepB"
                     /locus_tag="VF240_20425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7177636.1"
                     /translation="MSKSTRAINETAGKLPAAAKDDAGEAEATPHQHHSVLREYFESA
                     VVTVIMALFFMTFVAQAATVPSASMENTIYVGDHFLINKFIFAPGPHVPFLPQRDVRR
                     GDVVVFRYPAESIPRDQIFQYKTYFIKRVIGLPGETIEVQGSRVFVDGKELPEHSIAY
                     HDPRRGDDKAELKSLTAPPKSDDSLYSVYYSSLVTDDDRQTYLSVGMSYGVHEPYKLK
                     PDEYFMMGDNRDNSKDSRFWGPVKRDLVVGRAMFVIWSYDESKPKDDTLLIGPVINLF
                     NNTRWGRTGTLIR"
     gene            complement(1791..2168)
                     /locus_tag="VF240_20430"
     CDS             complement(1791..2168)
                     /locus_tag="VF240_20430"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX7177637.1"
                     /translation="MKLFSVEEANELLPKVGHIVRSIRRAYARVGSMREAAQAAAEGA
                     KEGGGGMTEGPQYVATLHALAERVAELDALGIQLKDYERGLIDFPHLREGRVVLLCWQ
                     LGEGKRLEWWHETDTGFAGRQPL"
     gene            complement(2261..3085)
                     /locus_tag="VF240_20435"
     CDS             complement(2261..3085)
                     /locus_tag="VF240_20435"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7177638.1"
                     /translation="MAVFIDGNNLFHAARFHNIDIDYNKLLRVLLGDGRLLRAFFYTG
                     VDVGAERQQGFLLWMRRNGFRVIHKELKTFYDGTRKANLDVEIAVDMLSLAGRYDTAV
                     LVSGDEDFVYAVNAVAYKGCRVEVAGFRSNTAPKLIDVADYFIDLGEIADKVRKDAPH
                     GSRYYDNNHAQETNHQPAYVPHDHNPVREAGAESHSNNSHGAAAARHPEGRDRRNFQQ
                     QQNNNHAHREPPRETATTPAAAPPEHVSESPEPAPPEPPPFEMPGGGDFIRVTDDQ"
     gene            complement(3302..4204)
                     /locus_tag="VF240_20440"
     CDS             complement(3302..4204)
                     /locus_tag="VF240_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177639.1"
                     /translation="MSRRTLRREPRALARAFVCALLAATVCGCAAASRGADPSQPRSE
                     SSYPITLAASEERAKAAGDAWARLAGERGVATPPQPEWQPITATLRALPQASGPPLLL
                     PRIGGEEGKAPTDEETREALRRFIAGAAPLLGVVPAELSLIAYDDAGGPLKRATYQQN
                     PFLYPMRGGYGRVEVTFAEDRRVVGLSSTAIPGAEGLRRVLAASFASSKITAERAVAS
                     IAGRTLTYADAAGDTQTREVGAQGQTSVRELVVLPVRSKTDAGTLELRVAWEVAVGGG
                     APLLVYVDAITGEQVAVVQATEGE"
     gene            4588..5631
                     /locus_tag="VF240_20445"
     CDS             4588..5631
                     /locus_tag="VF240_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177640.1"
                     /translation="MAVEDEPHLINDSGATPGAGRRFSPEELVACEGCRRANPPTRMS
                     CLYCGAKLPLTETSAALRRPVLRPLEEWEQGHNVVLLPGGPPASPQTIDAAAELLHFA
                     APRLIEAVDSREALPIARAASAEEAKLIEERLDALGFNVLVVADDELAEEAGPTRRVR
                     KLEFTEDVLRAWTSGGEQASAPWGDVVLIAAGRIKTKRVEVEERSGRVRPGVEVVDAR
                     EIFADEAALEIHTARDAAGWRIMSGSFDYTCLGARMGVLALRNFETLIGELRARATGA
                     AFDGGYNRLRHLLSAAWPLAEREESGGLRRERPGKFNAEAVTVVSNDAQFTRYVRLRR
                     LLELRRRTTDVSA"
     gene            5628..6530
                     /gene="rlmB"
                     /locus_tag="VF240_20450"
     CDS             5628..6530
                     /gene="rlmB"
                     /locus_tag="VF240_20450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX7177641.1"
                     /translation="MRRGRKQHTGDEARRENTDASRGSAGTPGSSAAASRDEEARVYG
                     LVPVLEVLRAGSRPVDRITVAEGAQDSRLRELFELARGRGVLVRRVPRAELQRMAGGG
                     INHQGVVATVAAARYVSADELVERIAARVGTTEPPLALVLDGVEDPRNLGAIIRTAEC
                     AGVHGVFVPERRAAGLTETVAKAAAGALEYVPVARAGNLARLQEELKERGVWTVGTAV
                     RAGVDYTEWDWTNPCAVFLGGEGEGLRRLLRERCDVLVSIPLRGRIESLNVSVAAGVI
                     LYEAVRQRTARGAAAYTSRVISDE"
     gene            6643..7173
                     /locus_tag="VF240_20455"
     CDS             6643..7173
                     /locus_tag="VF240_20455"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7177642.1"
                     /translation="MFCPKCAAQNADDAKYCRGCGADISLVPQAITGQLAQRLNEEEE
                     SGRRGHRRKHREPANIENAARQFFLGLAFALVSLAILSWAPGGRVWWFWMLIPAFIMI
                     GGGVGMYLRVREERSRLAPPAYAPAPASLSSPPHATGLPPRRHTGEIVQPPSVTEATT
                     RHLNMPVERTPKDADF"
     gene            7255..7791
                     /locus_tag="VF240_20460"
     CDS             7255..7791
                     /locus_tag="VF240_20460"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7177643.1"
                     /translation="MFCPKCGVKNVEDAKFCRACGADISLVPQALTRSLPEGAFGMRN
                     VGEEESEGGKKRRYKPTKPPTLEKGLSKCMEGVAFLLIFLTGYFYFFGAFFIWIWLII
                     PALANFGEGVGQIIRARSEGGALPPRTTFDAGELTPPAAHAAVELPQRATSEIVAPPP
                     SVTEGTTRHLGAAGPAKR"
     gene            7820..9382
                     /gene="aroE"
                     /locus_tag="VF240_20465"
     CDS             7820..9382
                     /gene="aroE"
                     /locus_tag="VF240_20465"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7177644.1"
                     /translation="MSAARICVPVCVGRSDELPGAVARAAPFADVVELRFDCLEAGQL
                     WPALESINELLDSHSIPFIFTLRHAAHGGRMKHDAAERAQFQARLHKLLAARAKAGKS
                     VAEHYVDLEFDEDSAAEAANTFGDLCGVVCSHHDFDGIPDDLGELYGRMAATGAGVVK
                     IAVTANDATDCLAVFRLLERARRDGRAAVVLAMGEAGILTRILGPSRGTALTYGALDD
                     TQRTAPGQISAEELRRLYRVHSITRETLVCGLVGSPVAHSLSPHIHNTAFAARGIDAV
                     YIPLEVSDLRDFVRRMVATRSRELDWNLRGLSITAPHKSAIIEHLDWTEPKAREIGAV
                     NTLVIVGGELRGYNTDAAASLAPLRGELDLSAARVAVIGAGGAARALLWELRETGART
                     TLFARDAARAANTARQFDAAPAPLEGARFDGFDLVVNATPLGTRGRMEGETPAAAHQL
                     RGARFAYDLVYNPRETRFLREARAAGCRPLGGLPMLLAQAAAQFKLWTTQDAPLDVMR
                     EAAERKIKEVRG"
     gene            9454..10470
                     /locus_tag="VF240_20470"
     CDS             9454..10470
                     /locus_tag="VF240_20470"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX7177645.1"
                     /translation="MERYSRQILFEGIGADGQRRIREGRVLVVGCGALGSAQVETLAR
                     AGVGRLRVADRDFVEESNLQRQTLFTERDARERTPKAVAARQRVAEINSDVEVEAEVV
                     DVNNSNVERLIDGCDVVLDGTDNFATRYLLNDACVKRGRVWVYGAAVGSYGVTMTVRP
                     RVSPCLRCVFPEVPPAGSAPTCDTAGVVMPIISVVAAVQASEALKLLAGKSELLHGSL
                     MQFDMWQNEWRRVRLGAPAADCPACVLGRFESLEAEAGEFTTVLCGRDAVQVSPRNSG
                     PIDLEALAGRLKSAGEVRLNPYLVRLRAGEYELTVFADARAIVRGTDDAKLARSLYAR
                     YVGN"
     gene            10818..13082
                     /locus_tag="VF240_20475"
     CDS             10818..13082
                     /locus_tag="VF240_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177646.1"
                     /translation="MTKKNYLSRAARAVLLAAPLISFAATLPATAGGPVVWETNSRAE
                     LLNGEARGVSITDTGALMLAPRFTQLFDTEQAYVWSSTTDAAGAVYLGTGHDGKIFRV
                     GSDGRGALLYDAAELDVTALVVGRDGALYAGTSPEGKVYRITSDGKAEVYFDPPDKYI
                     WSMALAADGAIAVGTGDAGRLYRVSAAGAKPEASVLADINETHVISLALDRQGNLIAG
                     TDPSGLVLRVSPDGKVFALFDSPLREIHALAPAADGSVYALALSEAASGPRPVVPATA
                     QPATQGSTQTSGGVSVTITSGDIIDGTQGATQFGGQQTQSRSRNELQNARSAVFRVQP
                     DGSSDVLWSSNSVTAFSVAPAPQDGALIGTSDKGRVYHVSDDGRDTLLVQSSEDQISS
                     FLVRGREVFAASSNQGKLFRLGVEPATEGTYESPVRDARFVASWGRIRWRAQGQVELQ
                     TRTGNTERPDMTWSEWSAPYREAGGTQVSSPRARFIQWRAVLRAPAGVVLEADRTARV
                     EDVSLAYLPRNVAPEIVSVQTLPVGIALLPAIQVPIDPNLESSGLDPSLFGAPVQVPP
                     RRTFQRGAVSLQWQAEDRNGDQLEYSVFYRAVSESVYRPLRERQRENFLAVDGAALGD
                     GRFVFKIVATDAPDNAVGQALRGERLSEPVDIDNSPPRVRADAPAQAQAGAPPRVRFV
                     VEDAGGMVRRADVSVDGEAWHSVFPEDGIADSPRESYVLDLPASAAPGEHTVSLRAFD
                     ASGNVSSARVVVRR"
     gene            13318..13527
                     /locus_tag="VF240_20480"
     CDS             13318..13527
                     /locus_tag="VF240_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177647.1"
                     /translation="MMKNLKRYLATLVAICTLSAGALASDWQRRNDQKPPPKEQKEIP
                     REDKKPPPPREDNQNRGGDKKRGKP"
     gene            13863..14342
                     /locus_tag="VF240_20485"
     CDS             13863..14342
                     /locus_tag="VF240_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177648.1"
                     /translation="MTGDSFGFTVTTPRGARVYAVSQPRAELLRAVDSGLTELFAVAR
                     RHGYRAGLSHSDYTIFVARPDRTRNGDGAYSPDIALGAAQYAGSVYDKGGYVYAAGVV
                     LSSNPPAFAIADHDRDYGRVSDVARYEGEHIVLYNNDRRLYSQTYDHSRGGSHPILR"
     gene            complement(14439..15272)
                     /locus_tag="VF240_20490"
     CDS             complement(14439..15272)
                     /locus_tag="VF240_20490"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX7177649.1"
                     /translation="MLSSLNRFSLALLATLLLLASPALAQGGSDKQEAKPDDTVSEKG
                     FRGRIFELKHSNPEQLHAVIRPLGSGFRGATISSNREFKTITVRDFPENIAVMEEAIK
                     RLDVPEPARPDIEFRVHILIATNSAQSPGGATQLPAELNDVVRQLQTTLSYKNYHLMT
                     SQVVRGKEGPGGLGNKGVAELRLTPDTTANENPIFYEYRLNQIWLDAAGTNPLKVQLG
                     NFNFSMRIPLQTSAAGTLQYENVGFNTPVGMREGEKVVVGTTSMQDKGIIVVLVANVI
                     K"
     gene            complement(15304..16098)
                     /locus_tag="VF240_20495"
     CDS             complement(15304..16098)
                     /locus_tag="VF240_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177650.1"
                     /translation="MNCESNERLLALHAGGDLSAGQASALSAHLRTCEDCRRLAADFA
                     ASRELLGTHEPPEFDAAFFDAVRRNVLREIDDAPPPPAFARLTALLFGRRALALAACV
                     VFLFAAGALAPYLREGHRLTGDTPQQVADANGEAQQESDGPSVIDSPAIRDIKVKTTP
                     AVKLTASSSPRRTQIVARATHASRAKRSERVERPPSHVSDRAVAAEIARAAGVAPTLE
                     EAAPASEVAVLAAPAERKMLRIELQTGDPNVRIIWLTPQEPEKLPR"
     gene            complement(16095..16748)
                     /locus_tag="VF240_20500"
     CDS             complement(16095..16748)
                     /locus_tag="VF240_20500"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7177651.1"
                     /translation="MTENRPTGEAASTDAAAHVDALSALVARARTGDRAAFEQLMVCT
                     QHKVAATAWRLLGNREDARDAAQETFLRAYKYLKSFKTGQDFHGWLYRITVNVCRDMM
                     RARSASPAGSAAREQRGGEFEAATPWSHAEGGDAERAALLAQQRTIVRRALAALPEKE
                     RTAVVLRDLEGLATEEVARLMNTRPATVRSQISTARVKLKVFCEQILREKRPGGSGL"
     gene            17019..18218
                     /locus_tag="VF240_20505"
     CDS             17019..18218
                     /locus_tag="VF240_20505"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX7177652.1"
                     /translation="MRRIDLRTAQTARSSTIRDINRQIVLNYVREREPISRADIARET
                     ALQRSTVSTIVEELKTEGLIEEIGAGKSTGGRRPTLLRLRAAGALAIGVDITPTRTTI
                     ATCDLAGRVLRRVQFANSARPDEMVALVIDRVREMAVGETGQRVAGVGVSLPGLVDTE
                     TGRAVYIPFFRWRDWAIAEEVEEATGLTVRVDNDANAAALAELWFGRPEISDARDFIM
                     VLVAEGIGTGIVFDGQIYRGERGAAGEFGHMIVGQQAPTACSCGNFDCWEAFSSQRAA
                     VARYVSTSAPGGVAGDVNFEGLVECALGGGQAAIDALSETAHYLGIGISNLIVGLSPE
                     TVVVGGHITRAWSLISPSLGETIRRSVRRGLPSARIVASTLGDQPTLMGAISLVLART
                     FGLAEQQ"
     gene            complement(18310..>23179)
                     /locus_tag="VF240_20510"
     CDS             complement(18310..>23179)
                     /locus_tag="VF240_20510"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7177653.1"
                     /translation="GAVGDGVADDGPAIKRALDALADAGGGTLTVPLGRFAILTPVIK
                     DFRGKATNVTIRGVPSAIDPNPKGPGSELTIGLGLVSEFVVKTGPEADALSIVGLDTF
                     LITDLVFIGTPEAEADARIALGLYAIRDAVVRHCEFYGLSTQTFGGAVLHALESGLTL
                     DKSVFLGSTGNSGIRVPVVQNVTWKNFVMTQTIFADYGQRPNYYGKLGLGAPFSWVML
                     GNPAPTDNLSPRREAFFSNVFFDEGGFIGVASFPDYYDPSSQPADLIHVSNFRMNVSS
                     LNSYGIYIYRGVRNVLIERGEFMLSTNATSAVELVNAGDVILDKLICGAAADTIRADA
                     ATRRLTVINSTYNHLYSQAPSTKVINSADPDKDPVQYVRQRFLTTLAREADPAANYFW
                     ADLLLQCNDVATCETSRKNELSAYLTASPQPTFSLSGRATKENGSGLSGVSVNLSGSQ
                     AVAAEADASGNYIFAGLPTSGDYTLTPGRINYTFTPASTAVSRPAGNRTVNFPGKANR
                     YQINGRLANAAGAPIAGATVTLSGAETATATTDAGGNYSLGVGAEGSYTVTPTRVNYT
                     FTPTSRTFSNVVADQTANFTGTPGNYTITGRVTEGALGLAGVTVTLSGSMAAAKTTDA
                     TGNYSFTAIAEGNYTVTATRTHYTLAPPSVTVSNLLGNRTANFAATLNRHAIRGKVTN
                     AAGAPVAGATVTLSGSQTATAAADADGNYTLPNLPAGGSFTVTSTRQNYTFTPASRTF
                     DALGSDQTTDFTGALVSYNITGRVTAGGASLAGATVRLSGSQTGTTTADAGGNYTFTV
                     TAEGNYTVTPSRPNYTFTPASAAFNNLSANAAADFAGALVSYQISGRVTTGGSALAGA
                     TVTLSGSSAGVATTDAAGNYTFNVKAEGDYTLTPSHPRHDFAPAARTFSNISGPQAAD
                     FAGTLKTYTVSGRVANINNTGIPGATVILSGSSTGTATTNAAGSYFFEGLTIGGAYTV
                     TPTLTHHDLAPTSRTITNLESDQTADFVASFTKYKINGRVTKDGSGLPGVSVVLSGSQ
                     AGAATTDAGGNYSFTVTALGNYTVTPSHTNHAFTPASRTFDALGSDQTADFTGALVSY
                     QLTGRVSVGTLGLAGVIVTLSGSTAAIITTDAGGDYTFTVTAEGNYTVTPSLAHYVFP
                     SANAALNNVSANASADFAGALANYKISGHVTDEGAALGGVTVTLSGTRTGTATTDAAG
                     AFSFNGLPALGNYTVTPILANRAFSPSSRTVGGLESDQTAEFTTTQRRLISFGAAAYS
                     ASEAAGSVAVTIVRTGDISSAAEVVCSAVNGSAEQRTDLSPVIGTISFESGEASKSLD
                     IFITDDAYLEGAEQLTLMLSDPVGGTLAEQSTTTLTIADNDHAPQAANPIDDAQFFVR
                     QHYRDFFNREPDADGLRFWSGQILSCDADPGCVEDKRINVSAAFFLATEFQETGYFIH
                     RLYKAAYARAPRHVEEFLLDSRQVGLGLVVNDPGWQQLLEDNKAEFATVFVERPDFVN
                     AYPLALAPAEFVGRLNERAGAPLAQAELGPAVGEFAGAATSADTAARARALRLVVESD
                     ALRRRELNAAFVLMQYLGYIQRNPDEAPDTDLSGYNFWLTKLQDHGGDFHTAQMVRSF
                     LVSTEYRARFGR"
BASE COUNT         3705 a         7317 c         8199 g         3958 t
ORIGIN      
        1 gacctcgacc gcgggagact gttgcgtctc gccgcgcgcc tcgacctgcg gcctgaccct
       61 cggctcgtac tgctcctcgg ccacgcgcgc gacggccgtc gcggcggccc ttccgccgct
      121 cggcccggag cggcgcgcgg cgggctgctg gcgcgcacgc tcgatgcgct tgagggcggc
      181 agccaccagc gggttcaact ccggcgcgtc cggcagcggc gggacgaggc cgaggcgtga
      241 agcggcggag ccttcttccg agggcgcggc gtccgctgcc gagacgcccg aaggctcctc
      301 ggccggaagg ctcgcggtct cgtccacgtc cggcgcggcc tcgcgcgcct tccgcttctg
      361 aatctcgcgc acgcgctcgc tcaaatcctt gcgccactgc gggatgctgc gtgcgccggg
      421 aaattcgatc agggtcgagc cgcccgcggc cgcagccggc ctcgcaccgg cggcttcagg
      481 cgaaagttcc ggcgcgacat ccggggccat ttcttcggtt gcgtgtgtca tatcaccaga
      541 aaaatccggg gaaggggcac gggcgtcgga gccggccttt cgcggcgcgc tcacaaggcc
      601 ggtctcgtcg gcagacggcg gcgacgtgcg cgcggcacac tacacttctt cggaaccaaa
      661 ccccttgaga acaaacaatt gtcttcgaca aagacgatgg ggcggtgagc tgtaaactca
      721 agccctttgc ggagtcgcgg cggatgctat cagagattac tttaagtcgt caatcagaaa
      781 atatacaagg cactgtggtt gggccttccg gggctaccag ataatggacg gctggcggac
      841 acgctttagc ggatcagcgt gcccgtccgg ccccagcggg tgttgttgaa gaggttgatg
      901 acggggccga tgaggagggt gtcgtctttc ggcttgctct cgtcgtaaga ccagataacg
      961 aacatggcgc ggccgacgac gaggtcgcgt ttgacaggcc cccagaagcg gctgtctttg
     1021 ctgttgtcgc ggttgtcgcc catcatgaaa tactcgtccg gcttgagctt gtaaggctcg
     1081 tggacgccgt agctcatccc gacggataag tatgtctggc ggtcgtcgtc cgtgacgagg
     1141 gaggagtagt agaccgagta gagggaatcg tctgatttcg gtggggccgt gaggctcttc
     1201 agctcggcct tgtcgtcgcc gcgccgcggg tcgtggtagg cgatgctgtg ctcgggcagc
     1261 tccttgccgt ctacaaacac gcgcgagcct tgcacctcga tggtctcgcc gggcaggccg
     1321 atgacgcgct tgatgaagta ggtcttgtat tgaaaaatct gatcgcgcgg aatcgactcg
     1381 gccgggtagc ggaagacgac gacatcgccg cgacgcacgt cgcgttgcgg gaggaagggg
     1441 acgtgcggcc ccggcgcgaa gatgaacttg ttgatgagga agtggtcgcc gacgtagatg
     1501 gtgttctcca tcgacgccga ggggacggtc gccgcctgcg cgacaaacgt catgaagaag
     1561 agcgccatga tgacggtgac gaccgccgac tcgaagtact cacgcagcac gctgtggtgc
     1621 tggtgcggcg tcgcctccgc ctcgccagcg tcgtccttcg ccgccgccgg aagctttccg
     1681 gccgtttcat taattgctct tgtcgattta gacatttcca ctcgtaggcg ccgggacacg
     1741 ccccgcattc tttcaccggg cgcgagcgcc gcgccgcgct ccgcgatgct ctaaagcggc
     1801 tgccggcccg cgaagccggt gtcggtttcg tgccaccact ccaaacgttt gccctcgccg
     1861 agctgccagc agaggagcac cacgcggcct tcgcgcaggt gcgggaagtc gatgaggccg
     1921 cgctcgtagt ccttgagttg gatgccgagg gcgtcaagct cggccacgcg ctcggcgagt
     1981 gcgtggagcg tggccacgta ttgcgggcct tccgtcatgc cgccgccgcc ctccttcgca
     2041 ccctcggccg cggcctgcgc cgcctcgcgc atcgacccca cccgcgcata ggcgcgccgg
     2101 attgaacgca cgatgtgccc caccttcggc agcaactcgt tggcctcttc gacgctgaaa
     2161 agcttcataa gcttcgcgtg ataagtgatg agtgataagt gatgagaaag agaaagacag
     2221 gttttgtctt atcacttatc actcatcact tatcactggt tcattggtcg tccgtgacgc
     2281 ggatgaagtc gccgccgccg ggcatctcga agggcggagg ctcgggcggc gctggctcag
     2341 gcgactccga cacatgctcg ggtggcgcgg ccgcgggggt cgtggccgtc tcgcggggcg
     2401 gctcgcggtg cgcgtgattg ttgttttgct gctgttggaa gttgcgccgg tcgcgccctt
     2461 cggggtgacg cgcggcggcg gcgccatggg agttgttgct gtggctctcg gcgccggcct
     2521 cgcggacagg gttatggtcg tgtgggacgt aggccggctg gtggttcgtc tcctgcgcgt
     2581 gattgttgtc gtagtagcgc gagccgtgcg gggcgtcctt acgcaccttg tcggcgatct
     2641 cgccgaggtc gatgaagtag tcggccacgt cgatgagctt cggcgcggtg ttcgagcgga
     2701 agccggccac ctcgacgcgg cagcccttat aggcgacggc gttgacggca tagacgaaat
     2761 cttcgtcgcc cgagacgagc acggccgtgt cgtagcggcc ggcgagcgag agcatgtcaa
     2821 cggcgatctc cacgtcgagg ttggccttgc gcgtgccgtc gtagaaggtc ttcagctctt
     2881 tgtggatgac ccgaaagccg ttgcggcgca tccagagcag gaagccctgc tggcgctcgg
     2941 cgcccacgtc cacgcccgtg tagaagaagg cgcgcagcaa acgcccgtcg cccagaagga
     3001 cgcgcaggag tttgttgtag tcgatgtcga tgttgtggaa acgagctgcg tgaaaaaggt
     3061 tgttaccgtc gatgaaaacc gccactctgc ctctgtagcc taactgccag gaaacgttgt
     3121 tcgtaggatc aaaatgcata cttgtattgc ttctccgttt aagatttgga agctgcgttt
     3181 tcaaatatcg aggaagaccg aaaccggccg cccgacccgc cgggcggggt gaacgtcctc
     3241 attctgttta cattagccgc acgcagaagg caagtcgagt cgcgccgacg cggccgtcga
     3301 ttcattcacc ttcggtcgcc tgcacgacgg cgacctgctc gccggtgatg gcgtcaacgt
     3361 agacgagcag cggcgcgccg ccgccgacgg cgacctccca ggcgacgcgc aattccagcg
     3421 tcccggcgtc agtcttcgag cggacgggca ggacgaccag ctcgcggacg gaggtttggc
     3481 cctgcgcgcc gacttcgcgc gtctgcgtgt cgccggccgc gtcggcgtag gtgagcgtgc
     3541 ggccggcgat ggaggcgacg gcccgttcgg cggtgatctt gctcgacgca aacgaggcgg
     3601 cgagcacgcg ccgcagtccc tcggcgccgg ggatggccgt gctggagagg ccgacgacgc
     3661 ggcggtcttc ggcgaaggtg acttcgacgc ggccgtatcc cccgcgcatg ggatacagga
     3721 aggggttctg ctggtaggtc gcgcgcttga gcgggccgcc cgcgtcgtcg taggcgatga
     3781 gcgacagttc cgcgggcacg acgccgagca gcggggccgc gcccgcgatg aaacggcgca
     3841 gcgcctcgcg cgtctcctcg tcggtcgggg ctttgccctc ttcgccgccg atgcgcggca
     3901 ggagcagcgg cggccccgac gcctgtggca aggctcggag agtcgcggtt atcggctgcc
     3961 actcgggctg tgggggcgtg gcgacgccgc gctcgccggc gaggcgcgcc catgcgtcgc
     4021 ccgcggcctt cgcgcgctcc tcgctggcgg cgagcgtgat cgggtaagac gattcggagc
     4081 gcggctgcga cgggtctgct ccccgcgagg cggcggcgca cccgcaaacg gtggcggcca
     4141 gcagggcgca gacaaaggcg cgggccagcg cccgtggttc acgtcggagt gttcgtcggc
     4201 tcatgtgcgt cgcgcgagga agagaaaaga atgatgcgtg cgtgggggcg atagggatta
     4261 tacttcatgc gcgcgacaag gcaatcccga catcgcttgc cgcgcaagac gccggcccgc
     4321 gacttaagag cgcgtcgaac acctgtaagt gtagggcaag tccgcggccc gcagccgcga
     4381 ggaataatta acatggatgg acaggattaa caggatgtga agatttgaaa tctcaaatct
     4441 gaaatttaat catctgttct tcatcctgct catcctgccc atccatgtta attgctctgt
     4501 agctaagggc cgcggtcttg ccccgcccct acagtcgtga cggacgctaa gagattttct
     4561 aacggctcta atcacaaagt gaaacgattg gcagtcgaag acgagcctca tttgattaac
     4621 gactccgggg cgacgccggg cgcggggcga aggttctcgc ccgaggagtt ggtcgcgtgt
     4681 gagggatgcc gacgggccaa cccgcccacc cgcatgagct gtctctactg cggtgcgaaa
     4741 cttccgctga cggagacgag cgccgcgcta cgccgtcccg tgcttcggcc gctggaggag
     4801 tgggagcagg gccacaacgt cgtcctgctt cccggtgggc cgccggcgtc gccgcaaaca
     4861 atcgacgcgg ccgccgagct tttgcacttc gccgccccgc gtctcatcga ggccgtcgat
     4921 tcgcgcgagg ctctgcccat cgcccgcgcc gcctcagccg aggaggcgaa gttgatcgag
     4981 gagagactcg acgcgctggg cttcaatgtc ttagtcgttg cggacgatga gctggctgaa
     5041 gaagctgggc cgacccgccg cgtgcgtaag cttgaattca ccgaggacgt gttgagggcg
     5101 tggacgagcg ggggcgagca ggcgtcggcg ccgtgggggg acgtggtgct catcgccgcc
     5161 ggtcgcatca aaacgaagcg ggtggaggtc gaggagcgca gcggccgtgt gaggcccggc
     5221 gtggaggtcg tggacgcacg cgaaatcttc gccgacgagg ccgcgctcga aatccacacc
     5281 gcgcgcgacg ccgccggctg gcgcatcatg tcgggcagct tcgattacac gtgcctcggg
     5341 gcgcggatgg gcgtgctcgc gttgagaaac ttcgagacgc tcatcggaga gttgcgcgcg
     5401 cgcgccacgg gcgcggcctt cgacggcggc tacaaccgcc tgcgccacct gctgagcgcg
     5461 gcgtggccgc tggcggagcg cgaggagtcg ggcggcctgc ggcgcgagcg tccgggcaag
     5521 ttcaacgccg aggccgtgac ggtcgtcagc aacgacgcgc agtttacgcg ctacgtgcgc
     5581 ctgcgccgcc tcctcgaact ccgccggcgc accacggacg tgagcgcatg aggcgaggcc
     5641 gaaagcaaca cacgggcgac gaagcgcggc gcgagaacac agacgcatcg cgcgggagcg
     5701 cgggaacgcc gggtagcagc gccgccgcgt cgcgtgatga agaggcgcgt gtctacggac
     5761 tcgtgccggt gctcgaagtg ctgcgcgccg gctcgcgccc ggttgatcgc atcacggtcg
     5821 cggagggagc acaggattcg aggctgcgag agttgtttga gctggcgcgc ggacggggtg
     5881 tcctcgtcag gcgtgtgccg cgggcggagt tgcagcgcat ggcagggggc ggcatcaacc
     5941 atcagggtgt ggtcgccacg gtcgccgcgg cgcgctacgt ctcggcggac gaactcgtcg
     6001 aacgaatcgc cgcgcgcgtc ggaaccacag agccgccgct cgcgctcgtg ctcgacggtg
     6061 tggaagaccc gcgcaacctc ggcgctatca tccgcacggc ggagtgcgcg ggcgtgcacg
     6121 gcgtcttcgt gccggagcgt cgcgcggcgg ggctgacgga gacggtggcg aaggcagccg
     6181 ccggcgcgct cgaatacgtc cccgtggcgc gcgccggaaa cctcgcgcgg cttcaggaag
     6241 agttgaaaga gcgcggcgtg tggacggtgg ggacggccgt ccgcgccggc gtcgattaca
     6301 cggagtggga ttggacgaac ccgtgcgccg tctttctcgg cggcgagggc gaggggctgc
     6361 ggcgattgct gcgcgagcgt tgcgacgtgc tggtgagcat cccacttcgc gggcgcatcg
     6421 agtcgttgaa cgtctcggtc gccgccggcg tgattctcta cgaggcggtc aggcagcgaa
     6481 ccgcgcgggg cgcggctgcg tatacgtcaa gagtgataag tgatgagtga tgagtgataa
     6541 gaggctgtta gctaaccgcc aacagctaaa agctaacagc ttcaattctt tcttatcact
     6601 catcactcat cacttatcac taattgtgga gggagccgaa agatgttttg tccaaaatgc
     6661 gcggcgcaga acgccgacga tgcgaagtat tgccgcgggt gcggggccga catcagcctc
     6721 gtaccgcaag cgattaccgg ccagctcgcg cagagactta atgaggagga agagagtggt
     6781 cgccgtgggc accggcgtaa gcacagggag ccggcgaaca tcgagaacgc cgcgcggcag
     6841 ttctttctgg ggctggcctt cgcgctcgtc tcgctcgcca tcctctcatg ggcgccgggc
     6901 gggagggtct ggtggttctg gatgctcatc ccggcgttca tcatgatcgg cgggggcgtc
     6961 ggcatgtacc tgcgcgtgcg cgaggagcgc agccgtctcg cgccgcccgc gtatgcgccc
     7021 gcgcccgctt cgctctcgtc cccgccgcac gccacgggac tgccgccgcg ccgtcacaca
     7081 ggtgaaatcg tccagccgcc aagcgtcacg gaggcgacga cccgacacct caacatgccc
     7141 gtcgagcgca cgccgaagga cgccgacttt taagaacgca gactttgagc gtcgccttcg
     7201 acaacttgcc gactacaact gccggcgact atcgacgccg tggagtgaat cgaagtgttc
     7261 tgcccgaaat gcggagtgaa gaatgtggag gacgccaagt tctgccgggc gtgcggggcc
     7321 gacatcagcc tcgtgccgca ggcgctgacg cggagcctgc ccgaaggcgc gttcggcatg
     7381 cgcaacgttg gggaggagga gagcgagggc ggcaagaaac gcaggtacaa acccacgaag
     7441 ccgccgacgc tcgaaaaggg tctgtcgaag tgcatggaag gcgtcgcgtt cctcctcatc
     7501 ttcctcaccg gctacttcta tttcttcggc gccttcttca tctggatttg gctcatcatc
     7561 cccgccctcg ccaacttcgg cgaaggtgtt gggcagatca tccgcgcgcg cagtgagggc
     7621 ggcgctctgc ccccgcgcac aaccttcgac gccggcgaac tgacgccccc tgccgcgcac
     7681 gcggccgtcg aactccccca gcgcgcgacc tccgaaatcg tcgctccgcc gccgagcgtc
     7741 accgaaggca cgacccgcca cctcggcgcg gccggccccg ccaaacgcta atccacacgg
     7801 cacctgttat atttgaatca tgagcgccgc gcgcatctgc gtccccgtct gcgtcgggcg
     7861 ttctgacgaa ttgcccggcg cggtcgcgcg cgctgccccg ttcgccgacg tggtcgagct
     7921 gcgcttcgac tgcctcgaag cgggtcaact ctggcccgcg ctggagtcaa tcaacgagct
     7981 acttgattcg cacagcatcc ccttcatctt cacgctccga cacgccgcgc acggcggacg
     8041 catgaagcac gacgcggctg agcgagcgca gtttcaggcg cggcttcaca agctgctcgc
     8101 ggcgagagct aaggcgggga agtccgtcgc ggagcattac gttgacctgg agttcgacga
     8161 agactcggcg gcagaagcgg ccaacacgtt cggcgacctg tgcggcgtgg tctgttctca
     8221 tcacgacttc gacggcattc ctgacgacct cggagaactt tacggacgga tggcggcaac
     8281 tggcgcgggc gtcgtcaaga tcgccgtcac ggccaacgac gcgacggact gcctcgccgt
     8341 cttccgcctg ctcgaacgcg cgcggcgcga cggccgcgcg gcggtcgtcc tcgcgatggg
     8401 cgaggccggc atcctcacgc gcatcctcgg cccctcgcgc ggcacggcgc tcacctacgg
     8461 cgcgctcgac gacacgcagc gcaccgcgcc cggtcaaatc agcgcggagg aattgcgtcg
     8521 gctctaccgc gtgcattcga tcacgcgcga gacactcgtc tgcggcctcg tcggctcgcc
     8581 cgtcgcacac tcgctctcgc cgcacatcca caacaccgcc ttcgccgcgc gcggcatcga
     8641 cgccgtctac atcccgctcg aagtctccga cctccgcgac ttcgtgcgcc gcatggtcgc
     8701 cacgcgcagc cgcgagcttg actggaacct gcgcggcctg agcatcaccg cgccccacaa
     8761 gagcgccatc atcgaacacc tcgactggac ggagccgaag gcccgcgaaa tcggcgcggt
     8821 caacacgctg gtcatcgttg gcggagagct tcgcggttac aacaccgacg ccgcggcctc
     8881 gctcgcgccg ctgcgcgggg agcttgattt gagcgccgcc cgcgtcgccg tcatcggtgc
     8941 gggcggcgcc gcccgcgcct tgctgtggga actgagagaa accggcgcgc gcacgaccct
     9001 gttcgcccgc gacgccgcgc gggccgccaa tacggcaaga caattcgacg ccgcgcccgc
     9061 gccgctcgaa ggggcgcgct tcgacggctt cgacctcgtc gtcaacgcga cgccgctcgg
     9121 cacacgcggc cgaatggaag gcgaaacgcc cgccgccgca catcaactgc gcggcgcgcg
     9181 cttcgcctac gaccttgttt ataatccacg cgagacgcgc ttcctgcgcg aggcccgcgc
     9241 ggccggctgc cgccccctcg gcggcctccc catgctcctc gcgcaggccg ccgcacagtt
     9301 caaactctgg acgacacagg acgcgcccct cgacgtgatg cgcgaggcgg cggaaaggaa
     9361 aatcaaagag gtcagaggtt agaggtcaga ggttagtaaa aacctcttgt tctttaacta
     9421 acctctgacc tctaacctct gacctctttt cttatggaac gttacagccg gcaaattctg
     9481 ttcgagggca tcggcgccga tgggcagcgg cggattcggg aggggcgcgt gctcgtcgtc
     9541 gggtgcggcg cgctcggctc ggcgcaggtc gagacgctgg cgcgcgcggg cgtcggacgc
     9601 ctccgcgtgg ccgaccgcga cttcgtcgag gaatcgaacc tccagcggca gacgcttttc
     9661 accgagcgcg acgcgcgcga gcgcacaccg aaggccgtcg ccgctcgcca gagggtcgcg
     9721 gagatcaact cggacgtgga ggtcgaggcc gaggtcgttg acgtgaacaa ctcgaacgtc
     9781 gagcggctga tcgacgggtg cgacgtggtg ctcgacggga cggacaactt cgcgacgcgc
     9841 taccttctca acgacgcctg cgtgaagcgc ggccgggtgt gggtctacgg cgcggccgtc
     9901 ggctcctacg gcgtgacgat gacggtgcgg ccgcgcgtca gtccttgcct gcgctgcgtc
     9961 ttccccgaag tgccgccggc cggctccgcc ccgacgtgcg acacggcggg cgtcgtcatg
    10021 ccgatcatct cggtggtcgc ggccgtgcag gcgagcgagg cgctgaagct tttggccgga
    10081 aagagtgagt tgctgcacgg ctcgctgatg cagttcgaca tgtggcagaa cgagtggcgc
    10141 cgcgtccgtc tgggcgcgcc ggcggccgac tgtccggcgt gcgtgctggg gcgcttcgag
    10201 tcgctggaag cggaggcggg cgaattcacg accgtgcttt gcgggcgcga cgccgtgcag
    10261 gtctcgccgc gcaactccgg ccccatcgac ctcgaagctc tggcgggccg tctgaaatcc
    10321 gccggggagg tcaggctcaa tccgtacctc gtgcgcctgc gcgcgggcga gtacgagctg
    10381 accgtcttcg ccgacgcgcg cgccatcgtc cgcggcaccg acgacgcgaa gctggcgcgc
    10441 tcgctctacg cgcgttacgt cgggaactga aaacgtgccg acgattgtag ggcagggcaa
    10501 gtccgcggcc cgtcttaccg gaagaattat taacatggat ggagaggatc gataggatca
    10561 gaagatttga aatctcaaat ctgaaatctg caatcagatc tttctctatc ctacccatcc
    10621 tgtccatcca tgttaataat tctgtaagca cgggccgcgg acttgccttg cccctacggc
    10681 attgtcgaag tttcttatct ccggcgcatc ggttatgatt ccgtgtggtc gatgaattgc
    10741 cgaccgaggg agtcgcaact gttcagcccg tcgattcatc acatcccaaa acctcaaggg
    10801 gccatctaat tctgttgatg actaagaaaa actatctgtc ccgcgccgcc cgcgccgtcc
    10861 tcctcgccgc cccgctcatc agtttcgccg cgacgctgcc ggcgacggcc ggcggccccg
    10921 tcgtgtggga gacgaactcg cgcgccgaac ttctaaacgg tgaggcgcgc ggcgtgtcga
    10981 ttaccgatac cggcgcgctg atgctcgcgc cgcgtttcac gcagctcttc gacaccgagc
    11041 aggcttacgt ctggtcgagc acaacggacg cggccggcgc cgtctacctc gggacggggc
    11101 acgacgggaa gattttccgc gtcggctcgg acgggcgcgg cgcgctcctc tacgacgcgg
    11161 ccgagttgga cgtgacggcg ctcgtggtgg gacgcgacgg cgcgctctac gcgggcacgt
    11221 cgcccgaggg caaggtctac cgcatcactt cggatggcaa ggccgaggtc tacttcgacc
    11281 cgcccgacaa gtacatctgg tcgatggcgc tcgcggccga cggcgcgatt gccgtcggga
    11341 cgggcgacgc cggccggctc taccgcgtaa gcgcggccgg ggccaaaccg gaagcgtccg
    11401 tgctcgccga cattaacgag acgcacgtca tctcgcttgc gctcgaccgg cagggcaacc
    11461 tcatcgccgg caccgatccg agcggactcg tcctgagagt ctcgcccgac gggaaagttt
    11521 ttgccctctt cgactcgccg ctgcgcgaga ttcacgcgct ggcgcccgcc gccgacggct
    11581 ccgtctacgc gctcgccctg agcgaagcgg cgtcggggcc gcgccctgta gtgcctgcca
    11641 ctgcgcaacc cgctacgcaa ggctcgacgc agacgagcgg cggcgtctcc gtgacgatca
    11701 cctcgggcga catcatcgac ggcacgcagg gcgcaacgca gttcggcggc cagcagacgc
    11761 agtcgcgcag ccgcaacgaa ctccagaacg cgcgcagcgc cgtcttccgc gtccaacccg
    11821 acggcagctc cgacgtgctc tggagttcca acagcgtgac ggccttctcc gtggcgcccg
    11881 cgccgcagga cggcgctctc atcggcacca gcgacaaggg gcgcgtctac catgtgagcg
    11941 acgacgggcg cgacacgctg ctcgtccagt cgtccgaaga ccaaatctcc tcgttcctcg
    12001 ttcgcggccg agaggtcttc gccgcgtcga gcaatcaggg caagctcttc cgcctcggcg
    12061 tggagccggc gacggagggg acgtatgagt cgcccgtgcg cgacgcgcgt ttcgtcgcct
    12121 cgtggggacg catccgctgg cgcgctcagg ggcaggtcga gttgcagacg cgcacgggta
    12181 acaccgagcg ccccgacatg acgtggagcg agtggagcgc gccctaccgc gaggccggcg
    12241 ggacgcaggt cagtagcccc cgcgcgcgct tcatccagtg gcgggcggtg ctgcgtgcgc
    12301 cggccggcgt ggtgctcgaa gccgaccgca cggcgcgcgt cgaagacgtg agcctcgcct
    12361 atctgccgcg caacgtcgcg cccgaaatcg tctccgtgca gaccttgccg gtcggcatcg
    12421 cgctgctgcc ggccatccag gtgcccatcg atccgaacct cgaatcgtcg gggctagacc
    12481 catcgctctt cggcgcgccc gtgcaggtgc cgccgcggcg caccttccag cgcggcgccg
    12541 tctcgctcca gtggcaagcc gaagaccgca acggcgacca gctcgaatac tccgtcttct
    12601 accgcgccgt gagcgagagc gtctaccggc cgctcaggga gcgacagcgg gagaacttcc
    12661 tcgccgtcga tggcgcggcc ctcggcgacg gccgcttcgt cttcaagatc gtcgccacgg
    12721 acgcgcccga caacgccgtc gggcaggcgc ttcgcggcga gcgactgagc gagccggtgg
    12781 acatcgacaa ctcgccgccg cgcgtccgcg ccgacgcacc ggcgcaggct caggccggcg
    12841 cacctccgcg cgtccgcttc gtcgtcgagg atgcgggcgg gatggtcagg cgcgccgacg
    12901 tgagcgtcga tggcgaagcc tggcactcgg tcttccccga agacggcatc gccgacagcc
    12961 cgcgtgagtc ttacgtgctc gatctgcccg cctcggccgc ccccggcgag cacaccgtct
    13021 cgctgcgcgc cttcgacgcc agcggcaacg tcagcagcgc gagagtcgtc gtccggcgct
    13081 agccgttttg gcctccgggc gcatcaaaag ttcggcgatg cacgatacgg tgcccggcgt
    13141 gccggaaatc gttcggcaga ccgcaagcgg ctggaaataa agccgttcat gtgttgagtt
    13201 tcgcggcgtc ggccccagtg gcaccagcct tgccagtctt tagtggtgag accgcgcgtc
    13261 tctcctctcc ctcccatgcg cgcctccgta taaacggaca tgaggaggac aaaggttgtg
    13321 atgaagaact tgaagcgata tctggcgacg ctggtcgcaa tctgcacgct ctcggcgggg
    13381 gcgctggcct ccgactggca gcggcggaac gaccagaagc cgccgcccaa ggagcagaag
    13441 gagattccgc gcgaggacaa aaagccgccc ccgccgcgtg aagacaacca gaaccggggg
    13501 ggcgacaaga agcggggaaa accctagacg tgctcctgcc ggccgcccgt gaaattatcg
    13561 aggcggctga cgacaaggaa gtggtcacac gggggcacgt caaaaaatcc ttcagacgaa
    13621 ggtcgctatt cagaccgcag gcgcgccgta ggggagactg aaaagtttcc cggacaagcg
    13681 cgcctgtagt tgttttatca cgcatggagg gggaagcgat gaaactgagc aaccacggaa
    13741 gggcgctctc ggcgcttaca ctttgcgccg cattaatctt ttcactgggc ggcttcgcgc
    13801 cggccgcgaa cgctcagcgg cggggcggca ggggtggggg tctggtcgca cgcgcgcagt
    13861 caatgaccgg cgactctttc ggcttcacgg tcacgacgcc gcgcggcgcg cgagtctacg
    13921 ccgtcagcca accgcgggcc gagctgttgc gcgcggtgga cagcgggctg acggagttgt
    13981 tcgccgttgc gcgccgccac ggctaccgcg cggggctgag ccattcggac tacacgattt
    14041 tcgtcgcccg ccccgaccgc acgcgtaacg gcgacggcgc ctactcgccc gacatcgcgc
    14101 tcggtgcggc gcagtacgcc ggcagcgtct acgacaaggg cggctacgtc tacgccgcgg
    14161 gcgtcgtgct ctcgtccaat cccccggcct tcgccatcgc cgatcacgac cgcgattacg
    14221 gacgcgtctc cgacgtagcc cgctacgagg gcgagcacat cgtgctctac aacaacgacc
    14281 ggcggctcta cagccagacc tacgatcaca gccgcggcgg aagccacccg atcttgcggt
    14341 aagtttctcc acaaggtatg aggtgaacaa cgtgggcgcg ccggtcgatt tacggatcga
    14401 ccggcgcgcc caaaactttt tgtctgattt ctgtcaacct acttgatgac gttcgctacg
    14461 aggacgacga tgatgccctt gtcctgcata ctcgtggtgc cgacgacgac cttctcgcct
    14521 tcgcgcatcc cgacgggggt gttgaagccg acgttctcgt actggagggt gccggcggcg
    14581 ctggtctgca acggaatacg catgctgaag ttgaagttcc cgagctgcac cttcaagggg
    14641 ttcgtgccgg ccgcgtcgag ccagatttga ttcagccggt attcgtagaa gatcgggttc
    14701 tcgttggcgg tcgtgtcggg cgtcaggcgc agctcggcga cgcccttgtt gccgaggccg
    14761 cccggcccct ccttgccgcg caccacctgc gaagtcatca ggtggtaatt cttgtagctc
    14821 agcgtcgtct ggagttgtct gaccacgtcg ttcagctcgg cgggcagttg ggtggcgccg
    14881 cccggactct gcgcgctgtt cgtggcgatg aggatgtgga cgcggaactc gatgtcgggg
    14941 cgcgccggct cgggcacgtc gaggcgcttg atggcctcct ccatgacggc gatgttctcg
    15001 gggaagtcgc gcacggtgat cgtcttgaac tcgcggttgg aagaaatagt cgcgccacgg
    15061 aagccgctgc cgaggggacg aattacggcg tggagctgtt ccgggttgct gtgcttgagt
    15121 tcaaaaatcc gaccgcggaa gcccttctcg ctgaccgtgt cgtccggctt cgcctcctgc
    15181 ttgtcgctgc cgccctgcgc gagcgccggg gacgcgagca ggagaagcgt ggcgagcagc
    15241 gcgagtgaaa atctgtttag ggatgagagc atgttgcctc ctgtggctgg ttgcagtttc
    15301 gtcttaacgg ggaagtttct caggctcctg cggcgtgagc cagatgatgc ggacgttcgg
    15361 gtcgccggtc tgcaactcga tgcggagcat cttacgctcg gccggcgccg caagcacggc
    15421 cacttcggac gcgggcgcgg cctcttcaag agttggagcg acgcccgccg cccgcgcgat
    15481 ttcggccgcg acggcacggt cagacacatg acttggcggc ctctcgacgc gttcactgcg
    15541 cttcgcgcgc gaagcgtgcg tggcgcgtgc gacgatttgt gtgcggcgcg gcgacgacga
    15601 ggcggtgagc ttcactgcgg gcgtggtttt cactttgata tcgcgtatcg cgggcgagtc
    15661 gatcacggac gggccgtcgg attcctgttg ggcttcgccg ttcgcatcgg cgacttgttg
    15721 cggcgtgtct cccgtcaacc tgtggccctc gcgaaggtaa ggggccaacg cgccggcggc
    15781 gaagaggaag acgacgcagg ccgcaagagc gagcgcacgg cggccgaaca gcagggccgt
    15841 gagccgggcg aaggccgggg gcggcggggc gtcgtcgatc tcgcggagga cgttgcgacg
    15901 gacggcgtcg aagaatgccg cgtcgaactc gggcggctcg tgtgtgccga gcagttcgcg
    15961 gctcgcggcg aagtcggcgg cgaggcggcg gcagtcttca cacgtccgca agtgtgcgga
    16021 gagggcgctg gcttgtccgg cgctcaggtc gccgcccgcg tggagcgcga ggagtcgttc
    16081 gttactttcg cagttcatag acctgagcct ccgggccgct tctcgcggag aatctgctcg
    16141 cagaacactt tgagcttgac gcgcgccgtc gaaatctggg agcgcacggt cgcgggccgg
    16201 gtgttcatca gccgggccac ctcctccgtc gccagtcctt cgaggtcgcg gagcacgacg
    16261 gccgtgcgct ccttctcggg caacgcggcg agcgcccgcc tgacgattgt tctttgctgc
    16321 gcgaggagcg cggcccgttc ggcgtcgccg ccctcggcgt gcgaccacgg ggtcgctgct
    16381 tcaaactcgc cgccccgttg ttcacgcgcc gcggagcctg ccggcgacgc gctccgcgca
    16441 cgcatcatgt cgcggcagac gttgacggtg atgcggtaaa gccagccgtg gaagtcctgc
    16501 cccgtcttga aactcttcaa atatttgtag gcgcgcagga acgtctcctg cgccgcgtcg
    16561 cgcgcgtcct cgcggttgcc gagcagacgc caggccgtcg cggcgacctt gtgctgtgtg
    16621 cagaccatga gctgctcgaa ggccgcgcgg tcgcccgtgc gcgcacgcgc gacgagggcc
    16681 gagagggcgt caacgtgcgc ggcggcgtct gtagatgcgg cctcgccggt cggccggttc
    16741 tccgtcatca acatgagact cccggcgctc ccgcggctca tcgtcaattc tgtccctctc
    16801 gtactcacgt caatctctac cgcgcggcgc gggaaaacgc tgagaacttt ttccgccgcc
    16861 acgctgggcg cggcactgta cttacacgcc ggcgcacgcc tttcgttgcg gcgaagtggg
    16921 gcggacgttt caacggcgcg tgcgaggaat tgtgttgacg cggtgcgggg aaacctatat
    16981 ttaagggcgt ccacagtcgc ccggagagta ccccgaccat gcggagaatc gacctcagaa
    17041 cggcgcagac cgcgcgttcc tccaccatcc gggacatcaa ccggcagatc gtcctcaact
    17101 acgtgcgcga gcgcgagccg atctcgcgcg ccgacatcgc gcgcgagacg gcgctgcaac
    17161 gctcgaccgt ctcgaccatc gttgaggagt tgaagaccga ggggctgatc gaagagatcg
    17221 gggcggggaa gtccacgggc gggcgccggc cgacgctttt gcgcctgcgc gcggccgggg
    17281 cgctggccat cggcgtggac atcaccccga cgcgcacgac catcgcgacg tgcgatctgg
    17341 cggggcgcgt gctccggcgc gtgcagttcg ccaacagcgc gcgccccgac gagatggtcg
    17401 cgctcgttat cgaccgcgtg cgtgagatgg ccgtcggcga gacggggcag agggtggccg
    17461 gcgtcggcgt cagcctgccc ggtctggtgg acacggagac gggccgcgcc gtctacattc
    17521 ccttcttccg ctggcgcgac tgggcgattg ccgaggaggt ggaggaggcc acggggctga
    17581 ccgtgcgcgt ggacaacgac gccaacgccg ccgcgctcgc cgagctgtgg ttcgggcggc
    17641 ccgagatttc cgacgcacgc gacttcatca tggtgctcgt cgccgagggc atcgggacag
    17701 gcatcgtctt cgacggacag atttatcgcg gcgagcgcgg agcggccggt gagttcggac
    17761 acatgatcgt cgggcagcag gccccgaccg catgctcctg cggtaacttc gactgctggg
    17821 aggcattctc ttcgcaacgc gcggccgtcg cgcgctacgt ctcaacgagc gcgccgggcg
    17881 gcgtggccgg cgacgtgaac ttcgagggcc tcgtcgaatg cgcgctcggc ggcgggcagg
    17941 cggccatcga cgccctttcg gagacggcac actacctcgg catcggcatc tcgaacctca
    18001 tcgtcggcct cagccccgaa accgtcgtcg tcggcggtca catcacgcgc gcgtggtctc
    18061 tcatctcgcc gtcgctcggc gagaccatcc gccgcagcgt ccggcgcggc ctgccctcgg
    18121 cgcgcatcgt cgcttccacg ctcggcgacc agcccaccct catgggcgca atcagcctcg
    18181 tcctcgcgcg caccttcggc ctcgccgaac agcagtaaag taaaagccgt caatcgtcaa
    18241 tcgtgaatcg taaatcgagg ccgcttccta cgatttacga ttcacgattg atgattgacg
    18301 gcttttcgcc taacggccga agcgggcgcg gtactcggtg gagacgagga acgacctgac
    18361 catctgtgcg gtatggaagt cgccgccatg atcctggagc ttcgtgagcc agaagttgta
    18421 gccgctgaga tcggtgtcgg gcgcctcgtc gggattccgc tggatgtagc cgagatactg
    18481 catgaggacg aaggcggcgt tgagttcgcg gcggcggagc gcgtcgcttt cgaccacgag
    18541 gcgcagggcg cgggcgcgcg ccgcggtgtc ggcgctcgta gccgcgccgg cgaactctcc
    18601 gacggccggc cccagctcag cttgcgccag cggcgcgccg gcgcgctcgt tgaggcggcc
    18661 gacgaattcc gccggcgcga gcgcgagcgg gtaagcgttg acgaagtcgg gacgctcgac
    18721 gaagaccgtg gcgaactcgg ccttgttgtc ttcgagcagt tgctgccagc cggggtcgtt
    18781 gacgaccagg cccagaccca cctgccggga gtcgagcagg aactcttcga cgtggcgggg
    18841 cgcgcgcgcg taggcggcct tgtagagccg gtggatgaag tagccggtct cctgaaattc
    18901 ggttgcgagg aagaaggccg cggagacgtt gatccgttta tcctcgacgc agccggggtc
    18961 ggcgtcgcag ctcagaatct ggcccgacca gaaccgcagg ccgtcggcgt ccggctcgcg
    19021 gttgaagaag tcgcgatagt gctggcggac gaagaactgc gcgtcgtcga tggggttcgc
    19081 cgcctgcggg gcgtggtcgt tgtcggcgat ggtcagcgtg gtggtgctct gttcggcgag
    19141 cgtgccgccg acggggtcgc tcagcatcag cgtgagctgt tccgcgccct ctaagtaggc
    19201 gtcgtccgtg atgaagatgt cgagggactt gctcgcctcg ccagactcaa agctgatcgt
    19261 gccgatgacc gggctgaggt cggtgcgctg ctcggccgag ccgttgacgg ccgagcagac
    19321 gacctcggcc gcggaggaga tgtcgcccgt gcggacgatg gtgacagcga cgcttccggc
    19381 cgcctcggag gcgctatagg cggcggcgcc gaagctgatg agccgcctct gcgtggtcgt
    19441 gaattcggcc gtctggtcgg attcgaggcc gccgacggtg cggctcgacg ggctgaacgc
    19501 acggtttgcc agaatgggcg tgacggtgta gttgccgagc gcgggcaggc cgttgaaaga
    19561 gaatgcgccg gcggcgtcgg tcgtcgcggt tccggtgcgc gtgccggaga gggtgacggt
    19621 gactccgccg agggccgcgc cttcatccgt cacgtgcccg ctaatcttgt aattggcgag
    19681 cgcgcccgca aagtcggccg aggcattggc cgagacgttg ttcaaggcgg cgttcgcgct
    19741 cgggaagacg tagtgtgcga gcgaaggcgt caccgtatag ttgccctcgg ccgtgacggt
    19801 gaaggtgtag tcgccgccgg cgtcggtcgt gatgatggcg gccgtcgagc cggagagcgt
    19861 gacgataacg ccggcgaggc cgagcgtgcc cacgctcaca cgcccggtga gctggtagct
    19921 gacgagcgcg cccgtgaagt cggccgtctg gtcggagccg agagcgtcga aggtgcggct
    19981 cgctggggtg aaggcgtggt tcgtgtgtga gggcgtgacg gtgtagttgc cgagggccgt
    20041 gacggtgaac gagtaattgc cgccggcgtc cgtggtggcc gcgcccgctt gtgagccact
    20101 gaggacgacg gagacgccgg ggaggccgct gccgtctttg gtgacgcgac cgttgatctt
    20161 gtacttcgtg aaggaagcga cgaagtcggc cgtctggtca gactccagat ttgtgatggt
    20221 gcggctcgtg ggtgcgaggt cgtggtgcgt gagggttggc gtgacggtgt aggcgccgcc
    20281 gatggtcaga ccctcgaaga agtatgagcc agccgcgttg gtggtggcgg tgcccgtgga
    20341 cgagccggag aggatgacgg tcgcgccggg gatgccggtg ttgttgatat tcgcgacgcg
    20401 cccgctgacg gtgtaggtct tgagtgtgcc ggcgaagtcg gccgcctgcg ggccggagat
    20461 attgctgaac gtgcgggcgg caggggcgaa gtcgtggcgc gggtgcgagg gcgtaagcgt
    20521 gtagtcgccc tccgccttca cattgaacgt gtaattgccg gcggcgtcgg tggtggcgac
    20581 gcccgcggat gagccggaga gcgtcaccgt cgcgccggcg agggcgctgc cgccggtcgt
    20641 cacgcggccg gaaatctgat agctgacgag cgcgccggca aagtcggccg cggcgttggc
    20701 cgagagattg ttgaaagcgg cgctcgcggg tgtgaacgtg tagttggggc gcgatggcgt
    20761 caccgtgtag ttgccctcgg ccgtgacggt gaaggtgtag ttgccgccgg cgtccgcggt
    20821 ggtcgtgccc gtctgtgagc cggagagcct gacggttgcg ccggcgagac tcgccccgcc
    20881 ggcagtgacg cgcccggtaa tattgtaact gacgagcgcg cccgtgaagt cggtcgtctg
    20941 gtcggagccg agagcgtcga aggtgcggct cgcgggggtg aaggtgtagt tctgccgtgt
    21001 ggaggtgacg gtgaagctgc cgccggccgg gaggttcgga agcgtgtagt tgccgtcggc
    21061 gtccgcggcg gccgtggcag tctgtgagcc ggagagtgtg acggtcgcgc cggcgacggg
    21121 ggcgccggcg gcgttcgtga ccttgccgcg aatggcgtgg cggttgagcg tcgcggcgaa
    21181 gttggcggtg cggttgccga ggagattgct gacagtgacg ctgggcggcg caagcgtgta
    21241 atgcgtccgg gtggcggtga cggtgtagtt gccctcggcg atggccgtga agctgtaatt
    21301 gcccgtcgcg tcggtcgtct tggcggcggc catcgagccg gagagcgtga cggtgacgcc
    21361 ggcgaggccg agcgcgcctt cggtgacgcg cccggtgatc gtgtaattgc cgggcgtgcc
    21421 cgtgaagttt gccgtctggt cggcgacgac attggagaac gtccggctcg tgggggtgaa
    21481 cgtgtagttc acgcgggtgg gcgtgacggt gtagctgccc tcggcgccga cgccgagcga
    21541 gtagttgccg cccgcgtccg tggtggccgt ggccgtctcg gcgccggaga gtgtgacggt
    21601 cgcgccggcg atgggggcgc cggcggcgtt ggctaagcgc ccgttgatct ggtagcggtt
    21661 ggccttgccg gggaagttca cggtgcggtt gccggcgggc ctgctgacgg ccgtggaggc
    21721 gggggtgaag gtgtaattga ttctgcccgg cgtgagggtg tagtcgccgc tcgtcggcag
    21781 accggcgaag atgtaattgc cgctcgcgtc ggcctcggcg gcgacggcct gcgagccgga
    21841 gagattaacg cttacgccgg agaggccgga gccgttctct ttggtggcgc gtccgctcaa
    21901 cgagaaggtg ggctgtgggg aggcggtgag gtaggccgac agctcgttct ttctggaggt
    21961 ctcgcacgtc gccacgtcgt tgcactggag gaggaggtcg gcccagaagt agttggcggc
    22021 cgggtcggcc tcgcgggcga gcgtcgtgag gaatcgctgg cggacgtact ggacggggtc
    22081 tttgtcgggg tcggcgctgt tgatgacctt cgtggacgga gcctgtgagt agaggtgatt
    22141 gtaggtcgag ttgatgacgg tgagccggcg cgtggcggcg tcggcgcgga tggtgtcggc
    22201 ggcggcgccg cagatgagct tgtcgaggat cacgtcgccg gcgttcacca actcgacggc
    22261 ggaggtggcg ttggtagaca gcatgaactc gccgcgctcg atgaggacgt tgcgcacacc
    22321 gcggtagatg tagatgccgt aggagttgag gctggagacg ttcatccgaa agttggagac
    22381 gtgaatgagg tcggccggct ggctcgaagg gtcgtagtag tcggggaagc tggcgacgcc
    22441 gatgaagccg ccctcgtcga agaagacgtt gctgaagaaa gcctcccggc gcggcgagag
    22501 gttgtcggtg ggcgcggggt tgccgagcat cacccatgag aagggggcgc cgaggccgag
    22561 cttgccgtag tagttgggtc gctggccgta gtcggcgaag atcgtctggg tcatgacgaa
    22621 gttcttccac gtcacgttct ggacgaccgg gacccggatg ccgctgttgc ccgtgctgcc
    22681 gaggaagacc gatttgtcca gagtcagccc gctctccaga gcgtggagga cagccccgcc
    22741 gaaagtctgt gtgctcaggc cgtagaactc gcagtggcgg acgaccgcgt cgcggatggc
    22801 gtaaagcccg agggcgatgc gcgcgtccgc ctcggcctcg ggcgtgccga taaagacaag
    22861 gtcggtgatg aggaaggtgt cgagtcccac gatggagagc gcgtcggctt cggggccggt
    22921 cttaacgacg aactcggaga caaggcccag cccgatggtc agctccgagc cggggccttt
    22981 ggggttgggg tcgattgcag acgggacgcc gcggatggtg acgttagtgg ccttgccccg
    23041 gaagtcttta atgaccggcg tgaggatggc gaaacggccg aggggcacgg tgagcgtccc
    23101 gccgccggcg tcggcgagcg cgtccaaagc cctcttgatc gcggggccgt cgtcggccac
    23161 gccgtcgccg acggcgccg
//
LOCUS       DASVES010000490        14900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_99158, whole genome shotgun sequence.
ACCESSION   DASVES010000490 DASVES010000000
VERSION     DASVES010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99158"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1679)
                     /locus_tag="VF240_20515"
     CDS             complement(105..1679)
                     /locus_tag="VF240_20515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HEX7177654.1"
                     /translation="MIHRSPFPDVEIPEVPLTEFVFRDAHLRADKPALIDGRDGRTLT
                     YAQLVEAIRRTAAGLARRGFGQRDVLAIYSPNAPEYPVAFHAAATLGGVVTTINPLYT
                     AEEVSCQLRDAGAKFLLSVPPLLGRAREAAAGTGVEEVFVFGEAEGATPFASLLEEPA
                     GELPPVRINSREDFVALPYSSGTTGVAKGVMLTHRNIISNLCQFKGAGYDWGDDTLIC
                     VLPMFHIYGLVAITNFGLYSGSTIVTLPRFDLEQVLGVMQDYRVTIAHLVPPIVLALA
                     KHPIVDRYDLSSLKTVFSGAAPLGEQLSRECCGRLGCNIIQGYGMTETSPVTHLTTHE
                     MSKHGSVGMCVSNTECKVVGLEAGEELGPGGEGEICVRGPQVMKGYLGRPEATAQTID
                     AEGWLHTGDIGYADREGFFFIVDRAKELIKYKGFQVAPAELEALLLTHPAVADTAVIP
                     SPDEEAGEIPKAFVVLKGGSDAAAEELMSFVAARVAPHKKVRLVEFIDQIPKSPSGKI
                     LRRVLVARERARLAGE"
     gene            complement(1835..3118)
                     /locus_tag="VF240_20520"
     CDS             complement(1835..3118)
                     /locus_tag="VF240_20520"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7177655.1"
                     /translation="MKQEFDKQLDSLLRGTRGGAGEVRRAAAAEHLSPDELSAYAENA
                     LPQAARSLYTAHLADCSDCRRIVTTVVLASGAEVAREARPAGVADVSRAASKSSWGAR
                     LAALFSPRVMAYAAPVLAVAIIGAVALVILRGRQGSEAPNVAQRSDEEAARPGSITQP
                     SPSAGTLEAAPAANIDAPAPQTQGSPAAAPGVGTAKTETTTNAPVKVKEMAQDKSNEL
                     ADADEFKTASPVPKPEVAAAAAAPPPASSQPAEAVPVAPTSTGARAPAREADDREVSR
                     QRRSAADNAREAERGEDARRDAPEPERGRAMNNNNERAERRVYDGKTKSDGNVPSETE
                     GLASARKRQGGPATGGGRGAASETRFVAGHEFRRQGNAWVDVNYRSSMSTVSVRRGSD
                     SFRSLAADIPEVGRAAGQLPGTVVIVARGRAYRIN"
     gene            complement(3191..4093)
                     /locus_tag="VF240_20525"
     CDS             complement(3191..4093)
                     /locus_tag="VF240_20525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7177656.1"
                     /translation="MPRPVEDSALVARMRSSLEKYLLRHRPDAPAPEVAKFLDALHAD
                     DLLLVLACEHGDEAAWEDLMRGFRSTVLSAARSASTGEAEAEELADSVWAELYGTRVR
                     ASDGRPAGKLAYYSGCGSLGGWLRAVVGQLGVDRHRRVAKLVQTEEASDFDRLADSEP
                     TGDGWRPATAPDPERALSETEAARAVADALARSVAQLDDEDRLLVRLYYFDGLRLKEA
                     GAVLGVHEATASRRLTRVHGEMRKRVETILTSECGWTREETAATLAQAAALLDTDLAS
                     LVTSEPGVAPEGRAGAVEARQPDG"
     gene            4357..5001
                     /locus_tag="VF240_20530"
     CDS             4357..5001
                     /locus_tag="VF240_20530"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7177657.1"
                     /translation="MLRVTEIFHSIQGESTHAGRPCAFVRLTGCPMRCVWCDSEYTFT
                     GGERMTIEDVLERVRAYGCRLVEVTGGEPLAQAGAFELIARLCEEGYEVLIETGGYVS
                     TEAVDERAKLILDVKCPASGEAERNHWPNLERLRADRDEVKFVVADRGDWDYTLEIIR
                     RYDLTTTARSVLISPVWGETDLRELAEWIKESGLDVRMQLQLHKYVWGAETRGV"
     gene            5023..5847
                     /gene="queC"
                     /locus_tag="VF240_20535"
     CDS             5023..5847
                     /gene="queC"
                     /locus_tag="VF240_20535"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX7177658.1"
                     /translation="MRDEINTGTENVPRSPPLPAGPPHAFGGWDEKAFDGPADDAKES
                     SVSSLITHHSSLSVCLVSGGMDSCVTAAIAREESEGLAFLHVSYWQRTQERERRAFEE
                     LADHYGVERRLVVSLEHLARIGGSSLTDPAIPVAEADLRARAIPTSYVPFRNAHLLAV
                     AVSWAEVIGALRVYIGAVAEDSSGYPDCRPEFYEAFQRVVDVGTKPETSVEIRTPVIR
                     MRKAEIIRRGVELRAPLHLTWSCYQAETRACGRCDSCALRLRAFREAGVTDPIPYA"
     gene            6007..7293
                     /locus_tag="VF240_20540"
     CDS             6007..7293
                     /locus_tag="VF240_20540"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177659.1"
                     /translation="MRIKLFFRTAAVALLLAATGLVASAQTLVVMGKVTMKQADGTEK
                     PVEGAQIDLIRTDIKQEFHTKTNKKGEYTHAGMPYGTFMIAVSAPGARPAYVANVPVA
                     RQPNNNFSLQPGDGSKLTLEQMKAGVTTGPAAAAAPPTETADAKKAREDYEKEVARVA
                     EANKKIEDSNVVVKREFEAGNTAFTAKNYDEAVAAYDRAIAAQPDQAVVHLNKSIALR
                     IRAVEKYNTAAKAKDKAGTEAARADFKNAAESADKAVKFYREKQGAAGAAPAAPAGAT
                     ELMGYLSARMEGYRLALQTNAGIDASLAVPAMEEYIVAETDPVKKAKAEAGLGNALFM
                     SGQIDASIAAYRKVLASNPTNLDALFGLGIALASDPEGKYAAEARDALKDFASKAPAT
                     DGRKADAESAVAALEESMKPKSAPKGDTGGKRRTRP"
     gene            7684..9498
                     /gene="pilB"
                     /locus_tag="VF240_20545"
     CDS             7684..9498
                     /gene="pilB"
                     /locus_tag="VF240_20545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX7177660.1"
                     /translation="MSAKLGEILVRENLLTPQQLREALDYQREHGGRLGFNLVKLGLV
                     ADDMITAVLSRQYGVPSVNLELFDIDETVLRLIPREVAEKYSVLPLSRVGATLTLAMV
                     DPTNVFAMDDIKFMTGLNIEPVVVSEAGVQGAIDNYYDQSREIELVGASDDYDHAHGN
                     GNGNGNGNGHFDLPSGSLTLDQLDFEEAAEAAEGVEVIEDNDEIDLSNLTRMSEDAPV
                     VRLVNVLMVDALSRGASDIHVEPYEKDLRIRFRIDGVLYDVMHPPLRLRDALVSRIKI
                     MSKLDISEKRLPQDGRIKIKLKLNGRSRELDFRVSTLPTLFGEKVVLRILDKDKLMLD
                     MTKLGFEPESLVKFQRNITKPYGMVLVTGPTGSGKTNTLYSALQSLNTSETNIMTAED
                     PVEFNLPGINQVQMKEQIGLNFAAALRSFLRQDPNIVLVGEVRDFETAEIAIKAALTG
                     HLVLSTLHTNDAPSTISRLMNMGIEPFLVATSVNLIQAQRLIRRVCSECKTQNPVPAE
                     ALIEVGFSPAEAKEVKTYKGVGCKICNNTGYKGRIGLYEVMEVTDDIRELILIGASAL
                     ELRKRAIEDGMISLRESGLQKIRNGVTTVEEVVRETVA"
     gene            9504..10895
                     /locus_tag="VF240_20550"
     CDS             9504..10895
                     /locus_tag="VF240_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177661.1"
                     /translation="MKLLRRLILSSLILALVATGLPVRDALARRSRRAVAAAHRRGNH
                     RHCRHTRAWWRRHRGRIRARQEQAMLLRRRRQQAALEAARERAFLTTPSFRTAAVPAL
                     NLAPVLRALPVTFPAPAPATFAPLVNNNVAPSLRNVAPSLRNVAPSLPDVKITRPAAA
                     SFPAASLPIAPHATNSALPSVTEDDASTAPAPRVELKSATPAGVAHAPAAAHITPTPA
                     FKQVVPAPASLNVAAPVAPLKSVAPAPKAERTVSPTVRAAERAARTPVVEPTPIIEPS
                     FRKTRAVRSEVSGARNPFDFAIPQAWRLSGANARGEMKFSVSGHDGRAAGTATLAPVS
                     ITGASAALDVITPRTRLIGGVALPVLRRTIIDRMVVEGGWVTNDTEREVDGRRVYVVT
                     AQKGQAGVARESWTFYFTEVDGRLYSLATNAPVEHSAPLAAGSEQFLGALRAGKSGIL
                     AAKPQRRSNPTND"
     gene            10905..12119
                     /locus_tag="VF240_20555"
     CDS             10905..12119
                     /locus_tag="VF240_20555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX7177662.1"
                     /translation="MNANEAAQEPLSLSDLLKKLIEMGGSDLHLTTNSPPQVRVDGTL
                     RALEGFRQLSSSDTKSLAYSVLTDAQKHRFEEALELDFSFGVRSLSRFRANLFNQRGA
                     VGAVFRAIPYEIKSFDDLGLPPVVSTLCDKPRGLILVTGPTGSGKSTTLAAMLDKVNR
                     ERHEHIVTIEDPIEFLHSHKNCLVNQREVGSDTKGFAEALRTALRQDPDVVLVGEMRD
                     LETIESALRIAETGHLTFATLHTNSAASTINRIIDVFPSAQQAQIRAQLSLVLEGILC
                     QSLLPRADGRGRGMALEILVPNAAIRNLIREDKVHQIYSMMQTGQGKYGMQTFNQSLA
                     TLYHKRLITLEVAMQRSQNTDELRELIEHGAGVNTGNGAGAGQRPGVPSAGHPSPYAQ
                     GRPIGTRPNGSR"
     gene            12262..13491
                     /locus_tag="VF240_20560"
     CDS             12262..13491
                     /locus_tag="VF240_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7177663.1"
                     /translation="MPTYVFKGRNRVGEVIVGERLADSRDVLRQLLRREQVMLTSVKE
                     KGREVAVPKIGGRKKVKAKELSIFTRQFSVMIDAGLPLVQCLDILGQQQENKYFAHLL
                     TQVRQDVEEGATLATAMSRHPKAFDQLFVNMIDAGETGGILDLILQRLSTYIEKIVKL
                     RRDVISALIYPSAVILIAIGVIAVIMVVVIPAFQNIFLGLLGPGEQLPLPTRIVVGMS
                     NFLAGIGGLIILAAVIAFVIAIRSYYKTKGGRWQLDSLMLKLPILGGILRKIAISRFS
                     RTLSTLLSSGVAILQALDITAKTAGNVVLEEAIQKIRTGVERGESFVEPLRASTVFPN
                     MVAQMVGIGEQTGALDSMLGKIADFYEQEVDSAIASLLTLIEPVMIAFLGVTIGSIVI
                     AMYMPLFTLIGKMSSGN"
     gene            13615..14097
                     /locus_tag="VF240_20565"
     CDS             13615..14097
                     /locus_tag="VF240_20565"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX7177664.1"
                     /translation="MNETDLNYVRRAVELAHEAERSGNVPVGALITLGGVVVAEAGNA
                     VLVPDYHPGRHAEMEALGRVSVELWPRAREMTCYTTLEPCVMCAGALLLHGVGRVVFG
                     STDAEGGAGPLLSRLPRYYAGGRGVPRWVGPVLPDVCDQLYRRACERFDRLPCGRDNF
                     "
     gene            14462..>14900
                     /locus_tag="VF240_20570"
     CDS             14462..>14900
                     /locus_tag="VF240_20570"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7177665.1"
                     /translation="MRTPSLRKLAAVFVSLCAAALFAASSAPPARAQAQITTGVIQGT
                     ITDEKDAVIPGAAVEAKNLDTNLTRTFSTDDDGRFAILQLPPGRYTVTVSKPGFATLA
                     QQEFQLTVGQAITLNLSMRPSQVQETVTVSAVTTIDTAKTEAST"
BASE COUNT         2557 a         5257 c         4647 g         2439 t
ORIGIN      
        1 ggtgcgagcc catcaaggga atcgcaccgg gcgtcagccg gcccgacgct cgtcgctgag
       61 tcttgatttg ggtggcggtt aatctcgccc ggccggcgtc cgcctcactc gcccgccaga
      121 cgcgcgcgct cgcgcgcgac gagcacgcgg cgcaggattt ttcccgatgg cgacttcggt
      181 atctggtcga tgaactcgac gaggcgcacc ttcttgtgcg gcgcgacgcg cgcggcgacg
      241 aagctcatca actcttccgc cgccgcgtca cttcccccct tgagcacgac gaaggcttta
      301 gggatttcgc ccgcctcttc gtccggcgag gggatgacgg ccgtatcggc gacggccggg
      361 tgcgtgagca gcagcgcttc gagttcggcg ggcgcgacct gaaagccttt gtacttgatg
      421 agttcctttg cgcggtcaac gatgaagaag aagccttccc ggtcggcgta gccgatgtcg
      481 cccgtgtgga gccacccctc cgcgtcaatc gtctgcgcgg tggcctcggg ccgtccgaga
      541 tagcccttca tcacctgcgg gccgcggacg cagatttcgc cctcgccgcc ggggccgagt
      601 tcctcgcccg cttccaaacc gacgacctta cactccgtgt tcgacacgca catgccgacc
      661 gagccgtgct tgctcatttc atgcgtcgtc aggtgcgtga cggggctggt ctccgtcatc
      721 ccgtatccct gaatgatgtt gcaaccgaga cgcccgcagc actcccgcga gagctgttcg
      781 cccagagggg ccgcgccgga gaagacggtc ttgagggatg agaggtcgta gcggtcaacg
      841 atggggtgct tcgcgagggc gaggacgatg ggcgggacga ggtgcgctat cgtcacccgg
      901 tagtcctgca tgaccccgag cacttgttcg agatcgaagc gcggcagcgt gacgatggtc
      961 gagccggagt agaggccgaa gttcgtaatc gccaccagcc cgtagatgtg gaacatcggc
     1021 agcacgcaga tgagcgtgtc gtcgccccag tcgtaccccg cccctttgaa ctggcacagg
     1081 ttggagatga tgttgcgatg ggtcagcatc actcccttcg ccaccccggt cgtcccgctt
     1141 gaatacggga gcgcgacaaa atcctcgcgc gaattgatcc gcacgggcgg caattcgccg
     1201 gccggctcct ctaataagga cgcgaacggc gtcgcgccct ccgcctcgcc gaacacgaaa
     1261 acttcttcga cgcccgtccc tgcggccgcc tccctcgccc tgccaaggag cggcgggacg
     1321 gacaggagaa acttcgcgcc cgcgtcgcgc aactggcacg aaacttcttc ggcggtgtag
     1381 agcgggttga tcgtggtgac gaccccgccg agcgtcgcgg cggcgtggaa ggcgaccggg
     1441 tactcgggcg cgttggggct atagatggcg agcacgtcgc gttgcccgaa gccccggcgc
     1501 gcgagaccgg cggcagtgcg gcggatggcc tcgacgagct gcgcgtaggt cagcgtgcgc
     1561 ccgtcgcggc cgtcgatgag cgccggcttg tcggcgcgca ggtgcgcgtc gcggaagacg
     1621 aattcggtga gcgggacttc gggaatctct acgtcgggga aggggctgcg gtgaatcatg
     1681 gggagagtct cctgcaactg ctcacgtcga gacgttggaa ttacgagcga ggcgaggcgg
     1741 tggatttcgc ggccgagttc gacgctacga ggctttggcg tctcccgcgg cgtgtctcgg
     1801 cgacggctcg catgccgttc tgttaatgac gccgtcagtt gattcggtac gcgcggccgc
     1861 gcgcgacgat gaccactgtc cccggaagct gcccggcggc gcggccgacc tcggggatgt
     1921 cggcggcgag cgagcggaag ctatcgctgc cgcgcctgac gctgacggtt gacatcgacg
     1981 acctgtaatt cacgtcaacc cacgcgttgc cctgtcggcg gaactcgtgg ccggcgacaa
     2041 agcgcgtctc cgacgccgcg ccgcgccctc cccccgtcgc cgggccgccc tgtcgcttgc
     2101 gggccgacgc caacccttcc gtttcgctcg gcacgttccc gtccgacttg gtctttccat
     2161 cgtagacgcg acgctcagcg cgttcgttat tgttgttcat cgcgcgaccg cgctccggct
     2221 cgggtgcgtc gcgccgcgcg tcctcgccac gctcggcctc acgcgcgttg tccgcggccg
     2281 acctccgctg ccgcgacact tccctatcgt ccgcctcccg cgcgggggcg cgagcgcccg
     2341 tggaagtcgg cgcgacggga acggcttccg ccggctgact ggaagccggg ggcggcgccg
     2401 ccgccgctgc tgccacctcc ggcttcggca ccggcgacgc tgtcttgaac tcgtccgcat
     2461 cggccaactc attcgattta tcctgcgcca tctccttcac ctttacaggt gcgttggtgg
     2521 tcgtctcggt cttcgccgtg ccgacgccag gcgcggccgc cgggctgccc tgagtctgag
     2581 gcgcgggcgc gtcaatgttc gcggccgggg ccgcctccaa cgtgccggcc gacggcgacg
     2641 gctgtgtgat ggaaccgggg cgcgcggcct cctcgtctga gcgttgcgcg acgttcggcg
     2701 cttcgctgcc ctgacgaccg cgaaggatga cgagcgcgac ggcgccgatg atcgccacgg
     2761 cgagcacggg cgcggcgtaa gccatcacac gcggcgagaa caaagcggcg agtcgcgcgc
     2821 cccacgaaga tttggacgcg gctcggctca cgtcggcgac gcctgcgggg cgagcttcgc
     2881 gagcgacctc cgcgccggac gcgagcacga cggtggtaac gatgcgtcgg cagtcagaac
     2941 agtcggcgag gtgcgccgtg tagagcgagc gcgcggcctg cggcagggcg ttctcggcgt
     3001 aggcgctcag ctcgtcgggc gagaggtgtt cggcggcggc ggcgcggcgc acttcgcctg
     3061 cgccgccgcg cgtgccgcgc agaagcgagt cgagctgttt gtcgaattcc tgtttcatcg
     3121 tgcgccctgc aaaaaaacgc atcgggtgcg ttcaaacaaa gaacgtgagc gagtgactaa
     3181 acttgcgccc tcagccgtcg ggttgacgcg cctcgacggc ccccgcgcgg ccctccggcg
     3241 cgacccccgg ctccgacgtg acgagcgagg cgagatcagt gtcgagcagc gcggctgcct
     3301 gcgccaatgt cgccgccgtc tcttcgcgcg tccagccaca ctccgaggtc agaatcgtct
     3361 cgacgcgctt tctcatttcg ccgtggacgc gcgtcaggcg gcggctcgcc gtcgcctcgt
     3421 ggacgccgag caccgccccg gcctccttca gacgcagccc gtcgaaatag tagagacgca
     3481 cgagcaggcg gtcttcatcg tcgagttggg cgacgctgcg ggcgagcgcg tcggcgaccg
     3541 cgcgcgccgc ctccgtctcc gacagcgcgc gctcggggtc gggcgctgtc gccggcctcc
     3601 agccgtctcc cgtcggctcg gagtcggcga gacggtcgaa gtccgaagcc tcctcggtct
     3661 gcacgagctt ggcgacgcgg cggtgcctgt ccacgccgag ctgcccgacg acggcgcgca
     3721 gccacccgcc gagcgacccg cagccggaat agtaagcgag cttcccggcg ggccgcccgt
     3781 cggaggcgcg gacgcgcgtg ccgtaaagct cggcccagac ggaatcggcc agctcctcgg
     3841 cctcggcctc gcccgtgctg gccgagcgcg ccgccgagag caccgtcgag cggaagccgc
     3901 gcatcaggtc ttcccaggcc gcctcgtcgc cgtgctcgca ggcgagcacg agcagaaggt
     3961 cgtcggcgtg cagggcgtcg aggaacttgg cgacctcggg cgcgggggcg tcgggccgat
     4021 ggcgcagaag atacttttcg agcgacgagc gcatgcgcgc gacgagcgcg gagtcttcga
     4081 cggggcgcgg cacggacgcc cgcgccctca gccgcaaagc accctctcgc aaaaattgtt
     4141 cgacactgtc cggcaccgtc acgcccacgc tccgtatgat gcctcttccg gcgggcacgt
     4201 atgctatcac aattcgccgc cgccacttcc tttgacacca ccggcgcggc agggtaaaag
     4261 cagtgagcag taagcagtga gcagtgagca gtcagaacac ataacctgtc ttgactgctt
     4321 actgctcact gctcactgct tactgcttcc tgccttatgt tgcgcgtcac cgaaatcttc
     4381 cactcgattc agggcgagtc cactcacgcc ggccggccgt gcgcgttcgt gcgcctgacg
     4441 ggctgcccga tgcgctgcgt gtggtgcgac agcgagtaca cgttcacggg cggggagcgg
     4501 atgacaatcg aggacgtgtt ggagcgggtg cgcgcttacg ggtgccggct cgtggaggtg
     4561 acggggggcg agccgctggc gcaagcgggg gcgttcgagt tgattgcgcg tttgtgcgaa
     4621 gagggttatg aagtcctcat cgagacgggc gggtacgtct cgaccgaagc ggtggacgag
     4681 cgcgcgaaac tcatcctcga cgtgaagtgc cccgcctcgg gcgaggccga gcgaaaccac
     4741 tggccgaacc tcgaacgact gcgcgccgac cgcgacgagg tgaagttcgt cgtcgccgac
     4801 cgcggcgact gggactacac gcttgaaatc atccgtcgct acgacctgac tacaactgcg
     4861 cgctccgtcc tcatctcgcc cgtctggggc gagaccgacc tgcgcgagct ggccgagtgg
     4921 atcaaagaga gcggcctcga cgtgcggatg cagttgcagc tccacaagta cgtctggggc
     4981 gcggagacgc ggggcgtgtg aagcagggat gaaggatgaa ggatgaggga tgaaataaac
     5041 acagggacgg agaatgtgcc gcgctcgccg ccgttgcccg ccggcccgcc ccacgctttc
     5101 ggcggctggg acgagaaggc tttcgacggg ccggcggacg acgccaaaga gagttctgtc
     5161 tcatcactta tcactcatca ctcatcactc tctgtttgcc tcgtctccgg cggcatggac
     5221 tcgtgcgtga cggcggcgat tgcgcgcgag gagtccgaag ggttggcgtt tctacacgtc
     5281 tcctactggc agcgaacaca ggagcgtgag cggcgggcgt tcgaggagtt ggccgaccac
     5341 tacggcgtcg agcgccggct cgtcgtctcg ctcgaacacc tcgcgcgcat cggcggctcg
     5401 tcgctcaccg accccgccat ccccgtcgcc gaggccgacc tccgggcgcg cgccatcccg
     5461 acctcctacg tccccttccg caacgcgcac ctgctcgccg tggccgtcag ttgggctgaa
     5521 gtgattggcg cgctccgcgt ctacatcggc gccgtcgccg aagactcatc gggctacccc
     5581 gactgccgcc ccgaattcta cgaggcgttc cagcgcgtcg tggacgtggg cacgaagccg
     5641 gagacgagtg tggaaatcag gacgcccgtc atccgcatgc gcaaggcgga gatcatccgt
     5701 cggggtgtgg agctgcgcgc gccgctgcac ctcacttggt cgtgctatca ggcggagacg
     5761 cgcgcctgcg ggcgctgcga ctcgtgcgcg ctgcggctgc gcgccttccg cgaggcgggc
     5821 gtgacagacc ccatccctta cgcctgaagt cggccccgga ggcttccctc cgcagtccgt
     5881 tttgctgcta tactttagga accgaaaagt tgacaaccgg cactgggtcg ctcgcatcct
     5941 acggagcggc ccgtcagaac cccaacaggc gcgtatcaac gctttccgca ggagaccact
     6001 gaagccatgc gtatcaaact cttctttcgc accgccgccg tcgcccttct gctggccgcc
     6061 accggcctcg tcgcttccgc acagacgctc gtcgtcatgg gcaaagtcac aatgaagcag
     6121 gccgacggaa ccgagaagcc ggtcgagggc gcacagattg acctcatccg gacggacatc
     6181 aagcaggagt tccacaccaa gaccaacaag aagggcgaat acacacacgc cggcatgccc
     6241 tacggcacct tcatgatcgc cgtcagcgcc cccggcgcgc gaccggccta cgtggccaac
     6301 gtccccgtcg cgcgccagcc gaacaacaac ttctcgctcc agcccggcga cggctcgaag
     6361 ctgacgctcg aacagatgaa ggccggcgtg accaccggcc ccgccgccgc cgccgcaccg
     6421 ccgactgaga ccgccgacgc gaagaaggcg cgtgaggatt atgagaagga ggtggcgcgc
     6481 gtcgccgagg ccaacaagaa gatcgaggac agcaacgtcg tcgtcaagcg cgagttcgag
     6541 gccggcaaca cggccttcac cgccaagaat tacgacgagg ccgtggccgc ctacgaccgc
     6601 gccatcgccg cgcaacccga tcaggccgtc gtccacctca acaagtcaat cgccctgcgc
     6661 atccgcgccg tggagaagta caacacggcc gccaaggcga aggacaaggc cggcaccgaa
     6721 gcggccaggg cagacttcaa gaacgccgcc gagtcggccg acaaggccgt caagttctac
     6781 cgcgaaaagc agggggcggc tggggccgcg cccgccgcgc ccgccggggc gaccgagtta
     6841 atgggctacc tctcggcgcg catggagggc tatcgcctcg cgctccagac caacgccggc
     6901 atcgatgcca gtctggccgt gcccgccatg gaggagtaca tcgtcgccga gaccgacccg
     6961 gtcaagaagg cgaaggccga ggctggcctg ggtaatgccc tcttcatgag tggacagatc
     7021 gacgcgtcaa ttgccgccta ccgcaaggtc ctggcctcga acccgacgaa cctcgacgcc
     7081 ctgttcggcc tcggcatcgc gctcgcctcc gaccccgaag gtaaatacgc cgccgaggcc
     7141 cgcgacgcgc tcaaggactt cgcctcgaag gcgccggcga ctgacgggcg caaggccgac
     7201 gccgagtcgg ccgtcgcggc gctcgaagag tcgatgaagc cgaagtcggc cccgaagggc
     7261 gacaccggcg gcaagcgacg cacccgtccg taaacgaacc gtcagagacc gcgcgatgag
     7321 ggcgactact gcatgcagta gtcgcccttt tccttttcgc ccgcactgct tttaccaccc
     7381 gcccgctatt tttgaacaat gacgaaatcc agcggccaat gctcggaggc cgacggcctt
     7441 cgcgcgtttt gagttcgcgt tttctccgcc ctcagcccgg cgattgcgtc gaaaagctcg
     7501 gaaaatattc accttgacag tcgtcccatt agtcagtaac atgctttggg caacggtgtt
     7561 ggccatcgtt gccttaagat aaacggctcg aaaggtccct gcttctcaac gtcccggcat
     7621 gcctggcccc ggagagtcgc ccgccccgtc cctctttctc aagcccgtga aaaatctcct
     7681 gaaatgtcgg ccaaactagg cgaaatactc gtccgcgaaa atctcctgac cccgcagcag
     7741 ttgcgcgaag cgctcgacta tcagcgcgag cacggcgggc gtctgggctt caatctcgtc
     7801 aagctcggcc tcgtcgccga cgacatgatc acggccgtgc tctcccggca gtacggcgtg
     7861 ccctcggtca atctggagtt gttcgacatc gacgagacgg tgctccggct catcccgcgc
     7921 gaggtggccg agaaatactc cgtgctcccg ctctcgcgcg tcggggccac gctcacgctg
     7981 gcgatggtcg atccgacgaa cgtcttcgcc atggacgaca tcaagttcat gacgggcctc
     8041 aacatcgagc cggtcgtcgt ctcggaggcg ggcgttcagg gcgccatcga caactactat
     8101 gaccagtcgc gcgagatcga actggtcggg gcgtccgacg actacgacca cgcgcacggc
     8161 aacgggaacg gcaatggcaa cggcaatggg cacttcgacc tcccgtcagg ctcgctcacg
     8221 ctcgaccagc tcgacttcga ggaggcagcc gaggcggccg agggcgtgga ggtcatcgag
     8281 gataacgacg agatcgatct ttcgaacctg acgcggatga gcgaggacgc ccccgtcgtc
     8341 cgcctcgtca acgtgctgat ggtggacgcc ctctcccgcg gcgcctccga catccacgtc
     8401 gagccttacg agaaagacct ccgcatccgc ttccgcatcg acggcgtgct ctacgacgtg
     8461 atgcacccgc ctctgcgtct acgcgacgcc ctcgtctcgc gcatcaagat catgtcgaag
     8521 ctcgacatct ccgagaagcg cctgccgcag gacggccgca tcaagatcaa gttgaagctc
     8581 aacgggcgct cgcgcgagct cgacttccgc gtctccaccc tgcccaccct cttcggcgag
     8641 aaggtcgtgc tgcgcattct cgacaaggac aagctgatgc tcgacatgac gaagctcggc
     8701 ttcgagccgg agtcgctcgt caagttccag cgcaacatca cgaagcctta cggcatggtg
     8761 ctcgtcactg gccccaccgg ctccggcaag accaacacgc tctactcggc cctccagtct
     8821 ctgaacactt ccgagacgaa catcatgacg gccgaagacc ccgtcgagtt caacctgccg
     8881 ggcatcaatc aggtgcagat gaaggagcag atcggtctta acttcgccgc cgccctgcgc
     8941 tcgttcctgc gccaggaccc gaacatcgtc ctcgtcggcg aggtgcgcga cttcgagacc
     9001 gccgagatcg ccatcaaggc cgcgctcacc ggccacctcg tcctctccac cctccacacc
     9061 aacgacgcgc cctccaccat ctcgcgtctg atgaacatgg gcatcgagcc tttcctcgtc
     9121 gccacttcgg tcaacctcat ccaggcgcag cgcctcatcc gacgcgtctg ctccgagtgc
     9181 aagacacaga accccgtccc ggccgaggcg ctcatcgaag tcggcttctc gcccgccgaa
     9241 gcgaaagagg tcaagaccta caagggcgtg ggctgcaaga tctgcaacaa caccggctac
     9301 aagggccgca tcggcctcta tgaagtgatg gaggtcaccg acgacattcg cgagttgatc
     9361 ctcatcggcg cgtcggccct cgagctacgc aagcgcgcca tcgaggacgg catgatcagc
     9421 ttgcgcgagt cgggtctgca aaagatcagg aacggcgtga cgaccgtcga agaggtcgtg
     9481 cgcgaaacgg tggcatgatg accatgaaac ttctacgtcg gctaatcctg tcttccctca
     9541 tcctcgcgct ggtcgccacg gggctgcctg tccgcgacgc cctcgcgcgc cgctcgcgcc
     9601 gcgccgtcgc ggccgcgcac cgccgcggca accaccgcca ctgccgacac acgcgtgcgt
     9661 ggtggcgtcg ccatcgtggg cgaattcgcg cgcgacaaga acaggccatg ctgttgcgtc
     9721 gtcgccggca acaggccgcg ctcgaagccg cacgcgagcg tgctttcctg actacgccct
     9781 ccttccgcac ggcggctgtc ccggctctta acctagcgcc cgtccttcgc gccctgcccg
     9841 tcacgttccc cgccccggcc ccggcgacct ttgcgccgct cgtcaacaac aacgtcgccc
     9901 cctcgctccg caacgttgcc ccttcactcc gcaacgttgc cccctcacta cccgacgtta
     9961 aaatcactcg cccggccgcg gcgagcttcc ccgccgcgag ccttcccatc gcgccgcacg
    10021 cgacaaactc ggccctcccg tccgtcacgg aggacgacgc ctcgacggcc cccgcgccga
    10081 gagtcgaact caaatccgca acaccggccg gcgtcgctca cgcaccggcg gccgcacaca
    10141 tcaccccgac gcccgcgttc aaacaggtcg tccccgcgcc ggcgtcgctg aacgtcgccg
    10201 cccccgtcgc gccgctgaag agcgtggccc ccgcgccgaa ggctgagaga accgtctccc
    10261 ccacggtgag agcagcggag cgtgcagcgc gtactccggt tgtcgaaccc actccaatca
    10321 tcgagccgag tttccgcaag acgcgcgccg tccgctccga ggtctccgga gcgcgcaacc
    10381 cgttcgactt cgccatcccg caggcgtggc gtctgtccgg cgccaacgcg cgcggcgaga
    10441 tgaagttctc tgtctccggc cacgacggcc gcgccgccgg cacggccacg ctcgcgccgg
    10501 tctctatcac gggcgcgtcg gccgcgctcg acgtcatcac gccgcgcacg cgtctcatcg
    10561 gaggcgtggc gctccccgtc ctgcgccgca cgatcatcga ccgcatggtg gtcgagggcg
    10621 gctgggtgac gaacgacacg gagcgcgagg ttgacggccg gcgcgtctac gtcgtcacgg
    10681 cgcagaaggg tcaggctgga gtcgcccgcg agtcgtggac gttctacttc acggaggttg
    10741 atggccgtct ctacagcctc gccacgaacg cccccgtcga acactccgcg cctctggccg
    10801 ccggctccga gcagtttctc ggggcgctgc gcgcgggcaa aagcggcatc ctcgccgcca
    10861 agccccaacg ccggtccaac cccactaacg actaagagga acgcatgaac gccaacgaag
    10921 ccgcacagga gccgctttcg ctctccgacc ttctgaagaa actgatcgag atgggcgggt
    10981 ctgatctgca cctgacgacc aactcgccgc cgcaggtgcg cgtggacggc accctgcgcg
    11041 cgctcgaagg cttccgccag ctctcctctt ccgatacgaa gtcgctcgct tacagcgtgc
    11101 tcaccgacgc ccagaagcat cgcttcgagg aggcgctcga actcgacttc tccttcggcg
    11161 tgcgcagcct ctcgcgcttc cgcgccaacc tcttcaacca gcgcggcgcg gtcggcgcag
    11221 tcttccgcgc catcccctac gagataaaat ctttcgacga cttggggctt ccgcccgtcg
    11281 tctccaccct ctgcgacaag ccgcgcggcc tcatcctcgt caccggcccg acggggtcag
    11341 gcaaatcgac gacgctcgcc gccatgctcg acaaggtcaa ccgcgagcgc cacgagcaca
    11401 tcgtcaccat cgaagacccc atcgagtttc tccattcaca caagaactgc ctcgtcaacc
    11461 agcgcgaggt cggctccgac acgaagggct ttgccgaggc gctccgcacg gcgctccgcc
    11521 aggacccgga cgtggtgctc gtcggcgaga tgcgcgacct tgaaacgatt gagtcggcgc
    11581 tgcgcatcgc cgagacgggc cacctcacct tcgccacttt gcacaccaac tcggcggcct
    11641 cgaccatcaa ccgcatcatc gacgtcttcc cctcggcgca gcaggcgcag attcgcgcgc
    11701 agctctcgct cgtcttggaa ggcatcctct gccagtcgct tttgccgcgc gccgacgggc
    11761 gcgggcgcgg gatggcgctc gaaatcctcg tccccaacgc cgccatccgc aacctcatcc
    11821 gcgaggacaa ggtgcatcag atttattcga tgatgcagac ggggcagggc aagtacggca
    11881 tgcagacctt caaccagtcg ctcgccacgc tctaccacaa gcgtctcatc acgctggagg
    11941 tggccatgca gcgttcgcag aacacggacg agctgcgcga gctgatcgaa cacggcgccg
    12001 gcgtcaacac cggcaacggc gcgggcgcgg gccagcgtcc cggcgtcccg tctgccggcc
    12061 acccctcccc ctacgcacag ggccgcccca tcggcacccg ccccaacggg agcagataaa
    12121 agcaggtgat gagtgatgag tgataagtga taagaagctg ttagctctta gcggttagct
    12181 aacagccaac agctaaaagc taactgcttc ttctctttct catcacttat cactcatcac
    12241 ttatcaccag gagttttcat aatgcccact tacgtcttca aaggccgcaa ccgggtcggc
    12301 gaggtgatcg tcggcgagcg cctggccgac agccgcgacg tgctccgcca gctcctgcgc
    12361 cgcgagcagg tgatgctcac ctccgtcaag gagaaggggc gcgaggtggc tgtgcctaag
    12421 atcggcgggc gcaagaaggt caaggccaag gagctgtcga tcttcacgcg ccagttctcc
    12481 gtgatgatcg acgccggact cccgctcgtg cagtgtctcg acatcctcgg ccagcagcag
    12541 gagaacaagt acttcgcgca cctcctcacg caggtcaggc aggacgtgga ggagggcgcc
    12601 acgctcgcca ccgccatgtc gcgccacccg aaggccttcg accagctctt cgtcaacatg
    12661 atcgacgcgg gcgagacggg cggtatcctc gacctcatcc tccagcgtct gtccacctac
    12721 atcgagaaga tcgtcaagct gcggcgcgac gtgatctcgg cgctcatcta cccgtcggcc
    12781 gtgatcctca tcgccatcgg cgtgatcgcg gtgatcatgg tcgtcgtcat cccggccttc
    12841 cagaacatct tcctcggcct gctcggcccc ggcgagcaac tgccgctgcc cacccgcatc
    12901 gtcgtcggca tgagcaactt cctcgccggc atcggcggcc tcatcattct ggccgccgtc
    12961 atcgccttcg tcatcgccat ccgctcctac tacaagacca aagggggccg ctggcagctc
    13021 gactcactca tgctcaagct gcccatcctc ggcggcatcc tgcgcaagat cgccatctcc
    13081 cgcttctcgc gcacgctctc gacgctgctc tcctccggcg tcgccatcct tcaggcgctc
    13141 gacatcacgg ccaagacggc cggcaacgtc gtgcttgagg aggcgatcca gaagattcgc
    13201 accggggtgg agcgcggcga gagcttcgtc gagccgctgc gggcctccac ggtcttcccg
    13261 aacatggtcg cgcagatggt cggcatcggc gagcagacgg gcgcgctcga ctcgatgctc
    13321 ggcaaaatcg ccgacttcta cgaacaggaa gtggactcag ccatcgccag cctgctcacc
    13381 ctcatcgagc cggtcatgat cgccttcctc ggcgtcacca tcggctccat cgtcatcgcc
    13441 atgtacatgc ccctcttcac cctcatcggc aaaatgtcct ccggcaattg atggcacgac
    13501 cgcgagtcgt ctcacggaca gccctccccc gaaccgcccg ccggcacttt cgaagccggc
    13561 gggcggttcg cctttgctcg cctcgcagtt gcccgccgcg tagaatgcga gccgatgaat
    13621 gagacggact tgaattacgt gcgccgcgct gttgagctgg cgcacgaagc ggagcggtcg
    13681 gggaacgtcc ccgtcggggc gctcatcact ctgggcggcg tggtcgtcgc cgaggcgggg
    13741 aacgccgtgc tcgtgcccga ctatcatccg gggcgtcatg cggagatgga ggcattgggt
    13801 cgcgtgagcg tcgaactctg gccgcgggcg cgtgagatga cttgctacac gacgcttgag
    13861 ccgtgcgtga tgtgcgccgg cgcgctgctg ctccacggcg tcggccgcgt ggtcttcggc
    13921 tcgaccgacg cggagggcgg cgcggggccg ttgctctcaa ggcttccgcg atattacgcg
    13981 ggcggccgcg gcgtcccccg ttgggtcggc cccgtgctcc ctgacgtgtg cgaccagctc
    14041 taccggcgcg cgtgcgaacg cttcgaccgc ctgccctgcg gcagagacaa cttctgacgc
    14101 tgacggaaaa agctgtcagt cgtccgttgt cagttgtcct cgtaggacga ctcggccgct
    14161 ctcactgaaa ggctcaggcc gtgggttcga gtacgactga ctactgactg ctgactactg
    14221 atagcttccg caacatgatg tgcggccgcg ggtattgaca cctgcgatgt agtgtcatta
    14281 agatgggccg cctcgcgcgc aagccaacct cgagcggctt ttcacacgta gttcagacag
    14341 ctttcagacc cgcacggcag cgccgcgccg ggcgtcgccc cgaggcgcgc accgcgcccg
    14401 ccgcgtgcag cccggtctaa cgaacaaaca cgtccgccac acggccgaca ggagacgagt
    14461 catgcgcacc ccttccctcc gtaaacttgc agcagtcttc gtgagccttt gcgccgccgc
    14521 gctcttcgcc gcgtcgtcgg ccccgccggc gcgggcgcag gcgcagatca cgaccggcgt
    14581 cattcagggc acgatcaccg acgagaaaga tgccgtcatc cccggcgccg ccgtcgaggc
    14641 caaaaacctc gacaccaacc tcacgcgcac cttctcgacc gacgacgacg gccgtttcgc
    14701 catcctccag ttgccgcccg gaaggtacac cgtgacggtg tcgaagcccg gcttcgccac
    14761 gctcgcccaa caggagtttc agttgaccgt cgggcaggcc atcacgctca acctgagcat
    14821 gcggccctcg caggtgcagg agaccgtgac cgtctccgcc gtcacgacca tagacacggc
    14881 caagaccgag gcgagcacca
//
LOCUS       DASVES010000491        18574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_682848, whole genome shotgun sequence.
ACCESSION   DASVES010000491 DASVES010000000
VERSION     DASVES010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18574
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682848"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VF240_20575"
     CDS             <1..930
                     /locus_tag="VF240_20575"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX7177666.1"
                     /translation="KVFFALGGNFLSATPDTEYTARALGRLRLTAHVSTKLNRAHLVT
                     GEQALILPCLGRTETDVQKSGPQFVSVENSMGVVHSSRGTLTPASPHLLSEPAIVAGL
                     ARAVLGRRGTLDWEWLVEDYDRVRDLIERAIPGFDDYNRRVRAPGGFYLPNAAREGQF
                     LTATKRANFTVHELPRHELAAGEFLMMTMRSHDQFNTTIYGLDDRYRGVHGERRVVFL
                     NEEDVREAGLSAGQVVDLISHFDGEERAARRFLVIPYQIPRRCAATYFPETNVLVPIG
                     SVADKSNTPASKSVRISIRPSDAPLRFDYNNIK"
     gene            complement(1021..1506)
                     /locus_tag="VF240_20580"
     CDS             complement(1021..1506)
                     /locus_tag="VF240_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177667.1"
                     /translation="MRARMFALGLALTVALAGITPAAAQDATARTSDWRAVQALTAGE
                     RVVVKTTDDGRKTGRFDSANDLQLVITRDGRRVTYARDRVRLVQINRGTSRGKGALLG
                     AAIGGGGGLAIGAGVYDSADGEFIGSIVPVIALVGAGIGAGIGALFGKGSKNVTIYEA
                     P"
     gene            1743..2315
                     /gene="efp"
                     /locus_tag="VF240_20585"
     CDS             1743..2315
                     /gene="efp"
                     /locus_tag="VF240_20585"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX7177668.1"
                     /translation="MALVGANELKRKMLIEVEGQPFAVVEVFHASPTARGATTMVRTR
                     LRNLLTGAVLEKSFRSGEKFAEPDVALAPASFLYSDAEGFHFMDEETYEQLMLTTEAV
                     GDDRGYLKDGLRLQILKYNGEAVSIELPQYVELAVVRTEPGVSGDRAAGRDLKPATLE
                     TGIDVRVPLFIKEGETVRVNTQTGEVSGRA"
     gene            2500..4638
                     /locus_tag="VF240_20590"
     CDS             2500..4638
                     /locus_tag="VF240_20590"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7177669.1"
                     /translation="MTSSKNRALIASLITCLLLLAPWPTARAADFKSEVIYQIITDRF
                     FDGSATNNNPAQSAGLYDATKTNWRAYWGGDLQGIQQKMSYLAGMGVTAIWISPPVDN
                     LNANIPDGSGNPTASYHGYQGRDFKRIEEHSGEQSNAWTAFDNLVTAAHQNGIKVIVD
                     FAPNHSTQDNAGEFGAIYDNGAFLGNYTNDVSGFFHHNGNIVNWDDRYQVQYYTLFNL
                     ADINQANPTMDAYMKASAQLFQQHGVDGFRIDAIKHINWGWQYSLANSIYTYGDSFLF
                     GEWYQTGTGDPLYRDSYKYANRSGISMLDFPLNTAIRNVFGSNANFSEIDTVLTAEAA
                     NFTWDEDLVTFIDNHDMTRFLSLNNNQTRLHQALAFILTQRGVPCIYYGTEQYLFNNT
                     SGGGDPYNRPMMNAFNTNTTAYTLTNRLSTLRRNNRAVPYGSHQQRWMNSDVYIYERK
                     FYGNVVLVAVNKGAAAVNVSGLNTALPVGAYNDYLTGLLSGVQISVASGTGGNNPVTT
                     FSLPANSVAVWQATETGGSPQVGSIGPTAAQPGVRVTIGGKNFGSTQGTVKFGTTTAT
                     VNSWSATKIVATVPAVTQGNYNVTVTNSASQVSNGIQFTVYQDKLIPVNFTVFNASPT
                     QVGDYIFLTGNTVELSNWATTWDAAVGPMLTPNYPNWFLTASVPAGKTIQFKFIKIAA
                     GGAVTWEAGANHSYTVPTSGTGFVNVNWQY"
     gene            4772..5431
                     /locus_tag="VF240_20595"
     CDS             4772..5431
                     /locus_tag="VF240_20595"
                     /inference="COORDINATES: protein motif:HMM:NF023380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HEX7177670.1"
                     /translation="MRHHSVVGLVLVLLSAVACAAQTQAAGAQAEGKPKPDPEALRKY
                     AGRYELEVGLIPVSTLDVTLEGGELFVKPSLVRKRKLIRKSKNVFRDEVEGARYRFQT
                     DDEGQVVGLTFPYEGAEYTAARVRLPPPSTSGNTTFRLKGHPGAQVVALAGAFNNWNQ
                     SQFLFGREGDEWVCRIDLEPGRYGYKFIVDGNWLLDPSNPETEEDAAGNINNVLEVKP
                     R"
     gene            5580..7265
                     /gene="ettA"
                     /locus_tag="VF240_20600"
     CDS             5580..7265
                     /gene="ettA"
                     /locus_tag="VF240_20600"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX7177671.1"
                     /translation="MSTQPNKVIYSMVGVSKHYDKRAVLKDIYLSYFYGAKIGVLGLN
                     GSGKSSLLKILAGVDKSFTGETVISPGHTVGYLEQEPRLDESRTVREIVEQGVQETVD
                     LLREYDEINARFGEEVSDDEMSKLLERQGAVQEKLDAVDAWDLDSRLEMAMDALRCPP
                     GDTSVSVLSGGEKRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLELHLQQYPGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKQERLRREEKQETERQKTLER
                     ELEWIRMAPKARRAKGKARINSYESLLNQQGEGRTRELEIYIPAGPRLGQVVIEADAV
                     SKAYGDNLLVEGMTFRLPPGGIVGIIGPNGAGKTTLFRMVTGQEQPDAGTIRVGETVE
                     LGYVDQSRDSLDADKSIWEEISGGQDVVKLGNREVNSRAYVGRFNFSGSDQQKKVSQL
                     SGGERNRVHLAKMLKSGANVLLLDEPTNDLDVNTMRALEEALENFAGCAVVISHDRWF
                     LDRIATHILAFEGDSRAVWFEGNYSDYEEDKRARLGDAAAQPHRIKYRQLTRG"
     gene            7299..7577
                     /locus_tag="VF240_20605"
     CDS             7299..7577
                     /locus_tag="VF240_20605"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX7177672.1"
                     /translation="MKATWNGSVIAESDDTVVVEGNQYFPADSITREHFRESDTHTTC
                     PWKGEASYYDVVVNGETNKDAAWYYPEPKDAAKEIKDHVAFWRGVKVE"
     gene            7591..8706
                     /locus_tag="VF240_20610"
     CDS             7591..8706
                     /locus_tag="VF240_20610"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7177673.1"
                     /translation="MIFRNRLTGRRARAAILSLFFVAALGAGVSAQQKKRPVKSPKPA
                     AVTPAVRAALEGVRADSLRGHLSFIASDLLEGRDTPSRGLDIAAEYVAAQFRRAGLEP
                     AGDDGYFQTATFALRSAPDTPIKSRNVVGLLRGSDPVLKDTYVMLSAHYDHVGVREQA
                     EGPDKIFNGANDDASGAVTVIELAGALAQLRPRPRRSILFVAFTGEEKGLQGSRFYGR
                     HPLVPLEKTVAQVNLEHMGRTDDSEGPQVGAFGVTGFDYSSVGATLQRASEAGGVRVY
                     KHPTNSDAFFRRSDNAALADLGVPAHTVSVSYIFPDYHGAGDHWEKIDYANMERVARA
                     LGLGLLALANDSQEPRWDEQNPKTERFVKAWRERRAK"
     gene            9029..12724
                     /locus_tag="VF240_20615"
     CDS             9029..12724
                     /locus_tag="VF240_20615"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7177674.1"
                     /translation="MPSLKSRRLLTSLILLATASALMLRAPSPVALAHHTPNPSSVTV
                     AGSLQSELGCSGDWQPECAATHLGYDSNDDVWQRVFSVPTGNWEYKAPLNDNWGENYG
                     LHAQQNGANIPLTLASPQSVKFYYDHKTHWITDNTTAVIAVAPGSFQSELGCSGDWDP
                     SCLRSWLQDPDGDGIYGFQTTALPAGNYEAKVAINESWDENYGQGGVPGGANIAFNVP
                     ANNTPVTFTYNATTHVLTIDSGSAPSGDVQWDGLRHDTFDPYYRSPFGAVPAGSNVTL
                     RFRTAHMDVSGVAVRVYTFDPATGATAGPADHPMTFLENRVEGGTTYDIWALTLTTPV
                     SPAVLYYKFRVNDQSDEDFYSDSYTDDHDNLNQGGEGAAFDGEPFPAFQITAYDPAFQ
                     TPAWLHDANVYQIFPDRFRNGDAANDYCRPGSSAGCPTFYGDQTPLLREPWNTAIGDP
                     RAAGPFQNQYGTQFFGGDLKGIENKLDYLQGLGVDTVYLTPIFKARSNHRYDTDDFLE
                     VDPALGGDAALASLRAEAERRGVRLILDGVFNHTSSDSRYFDRYHRYSPPPDGGCESA
                     ASPFRGWYNFFGVGPCDGNNYEAWFGFDSLPVLRDDSASVRDFFFRAPATNVTEYWYA
                     RGVSGWRFDVADNISHNWWREYRPYAKTYKADGPLVGEIWPDASQYLAGEQLDSVMNY
                     RFRKNVLGFARGAGWNDNDNSGGNQIAGLAPSQFDRALRSVREDYPEPATRAMLNLLD
                     SHDTNRALYVLTLLGDSGLAQAKERLKLSALFQFTYPGAPMVYYGDEGALDSPALALG
                     PNGPEDDPYNRAPYPWADEAGDTNVYGPVDTGVLDFYTKLGHMRKQFPALRTGSFETL
                     LTGDTSASAADNSTYAFARVSGASRAVVALNNGAAANTASLPVAAHFADGAELQDVLG
                     GATYTVSGGAVTVTLAARGGVVLLPAPAVIDTTAPAADIQTSPAPNANGWHDATPVTV
                     NLSATDAGSGVKELRYWVNGGAVTVAPGGGASLGLTQQGVYTVSLRAIDNAGNVSALA
                     ALVVRIDTTAPTVTVATPPGASADGNCQAAIPNLTPYTTASDNLTPAGSLVLTQNPAA
                     GTLVGLGAHTVTVTATDLAGHSTSASTTFTVSDNTPPAVTQPSASPSALTLPNHKMVE
                     VTLGYGASDNCAPATCALSVASNEPINGGDDGNTAPDWEVISPTLVRLRAERSGSGSG
                     RVYTITVTCTDPAGNPTVRSVTVTVPK"
     gene            complement(12927..13478)
                     /locus_tag="VF240_20620"
     CDS             complement(12927..13478)
                     /locus_tag="VF240_20620"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX7177675.1"
                     /translation="MAFKIGQKVVYPNHGIGIIEQIEQKQIGPTTLPFYTLRLAATNS
                     VVLVPVANAGEVGLRSPISSAECEMLVKALEDDFAPAAHDWKDRFKEFTEKMRSGCIF
                     EVAGVLKNLTYLSHLKPLSFREQRMLERARYLVISELAAVCRQPECNVTQRVEQALAR
                     ACTRHERRTPTRARVAAGAAAGH"
     gene            complement(13724..15049)
                     /locus_tag="VF240_20625"
     CDS             complement(13724..15049)
                     /locus_tag="VF240_20625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177676.1"
                     /translation="MNSRGWLAVTLCGALAAACAALYLRSPQTAAFSFCFLLAGLLAG
                     TLLARKVSQPAARDELSNGAGARDAVAPGVATPAVSGAVAAGEVPAGALLEATMDSMR
                     EGVIVIDSVMRVVASNDSAREIFDPGRGAVPRRLSELTRNPSVYSAFAGAVARGERVE
                     VKIETGEDGDRRVYDLQVAPLRHKEASFGSDVRGAVGVFFDITRLERLERVRQEFLSN
                     VSHELRTPLTAILTFIETLEAGGTDDPETTRRFLSVIRRNSERMRDLINDILELSAIE
                     AGTVTVEPVSVRLASLVQECFTALASCAAERGVELRSEVPAGVRVQADPRRLEQMLTN
                     LVDNAIKFNREGGSVTVAHERAGGRDRVHVSDTGEGISVEHLARIFERFYRVDRARSR
                     AMGGTGLGLAIVKHLARAHGGETLVRSAPGAGTTFTLELPAAPDTEASA"
     gene            complement(15343..16071)
                     /locus_tag="VF240_20630"
     CDS             complement(15343..16071)
                     /locus_tag="VF240_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7177677.1"
                     /translation="MSRPVLIVEDDPDIAEGLRYNLEREGLDARVALTGEQGLQLALD
                     QKSPPALVILDLMLPGMSGTELCRRLRREPATRRTPIIMLTARTSEAERVAGLDLGAD
                     DYISKPFSVRELMARVRAVMRRADESAAAGYEDARLSIDFGDMRVACDGEAVKLTRKE
                     FSLLATLARGAGRVATRQQLLDNVWGYEYYGDTRTLDVHIRRLRQKLGGCGDAIETVV
                     GVGYRFVGCAGKSEAAGVESSDVQ"
     gene            complement(16172..16831)
                     /gene="phoU"
                     /locus_tag="VF240_20635"
     CDS             complement(16172..16831)
                     /gene="phoU"
                     /locus_tag="VF240_20635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX7177678.1"
                     /translation="METHRRLDEELDVLRDRVLLLGGETETALQRAMYALSERDSGAA
                     REVLEGDTIIDLLEVEIDRLCIDVLALRQPAARDLRFVVAVAKVTPILERIADHACNI
                     ARAAIDLNDEPELRPLPDLPRMATTASQMLRSALDAFTRGDAADARRIIDRDDEIDEA
                     YNRVFHGLIETMTEDPSTTKRAARLLLVAKHLERVGDYVTDVCELTVYMTEAEIIKHS
                     H"
     gene            complement(16961..17809)
                     /gene="pstB"
                     /locus_tag="VF240_20640"
     CDS             complement(16961..17809)
                     /gene="pstB"
                     /locus_tag="VF240_20640"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7177679.1"
                     /translation="MSARSTRTQTPSASATAEQSGRGESATAAAAAKISVRHLNFFYA
                     KNQAVFDVSLDVPERVVMALIGPSGCGKSTVLRTLNRMNDIILGTRVEGQVLIDGRDI
                     YAPGTDVVSLRRRVGMVFQKSTPFPKSIYDNVAYGLRINRMAGSTRRETEERVRKALE
                     DAALWDEVKDRLKESALGLSGGQQQRLCIARALAVEPEVLLMDEPASALDPMATQKIE
                     ELIFELKRHYTIVIVTHNMQQAARVSDQTAFFWLGKLIEVNPTERMFTNPVQKLTEDY
                     ITGRFG"
     gene            complement(17815..>18574)
                     /gene="pstB"
                     /locus_tag="VF240_20645"
     CDS             complement(17815..>18574)
                     /gene="pstB"
                     /locus_tag="VF240_20645"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7177680.1"
                     /translation="SEVKADVQGLSFFYGQKQALNDINMTFPAHRVTAIIGPSGCGKS
                     TLLRCFNRIHDLYPANRYEGRITLQPSGVNIIAPETDPVEMRMRIGMVFQKPNAFPKS
                     VFENVAFGLRLMGEKGRAVEEKVEQALRSAVLWDEVKDRLKESAYSLSGGQQQRLCIA
                     RALATDPEMLLFDEPTSALDPIATSRIEELIFELKRQVTIIIVTHNMQQAARVSDFTA
                     FMYLGKLVEVAGTKKMFENPSVKLTEEYITGHFG"
BASE COUNT         3321 a         6404 c         5833 g         3016 t
ORIGIN      
        1 aaagtcttct tcgccctcgg cggcaacttt ctttcggcca cgcccgacac cgagtacacc
       61 gcacgcgcac tcgggcgctt gcgcctcacc gcgcacgtct cgacgaagct caaccgcgcg
      121 catctcgtca ctggcgagca ggcgctcatc ctcccgtgtc tgggccggac ggaaactgac
      181 gtgcagaagt cggggccgca gttcgtcagc gtcgagaact cgatgggcgt cgtccactcc
      241 tcgcgcggga ctctcacgcc agcgtcgccg catcttctga gtgagccggc gattgtggcg
      301 ggcctcgcgc gcgccgtgct cgggcgccgg ggcacgctcg attgggagtg gctggttgaa
      361 gactatgacc gcgtgcggga tttaatcgag cgcgccatcc ccggcttcga cgactacaac
      421 cggcgcgtgc gcgcgcccgg aggcttctac ctgcccaacg cggcgcgcga aggtcaattc
      481 ttgacagcga cgaagagggc caacttcacc gtccacgagt tgccgcgcca cgaactcgcg
      541 gccggcgagt tcctgatgat gacgatgcgc agccacgacc agttcaacac gaccatctac
      601 ggcctcgacg accgctaccg cggcgtccac ggcgagcgcc gcgtcgtctt cctcaacgag
      661 gaagacgtgc gcgaggctgg cctctcggcc gggcaggtcg tcgatctcat cagccacttc
      721 gacggtgagg agagagcggc gcgccgcttc ctcgtcatcc cctaccagat accgcgccgt
      781 tgcgccgcca cctacttccc cgaaacgaac gtcttagtcc ccatcggcag cgtcgccgac
      841 aagagcaaca cgcccgcgtc gaagtcggtt cgtatcagca tccgcccgtc ggacgcgccg
      901 ctcaggttcg actacaacaa catcaagtga gcagtgagca gtaagcagtg agcagtcaga
      961 aaacgcgaca tgttttgact gctcactgct cactgctcac tgcttactgc ttactgttct
     1021 tcacggcgct tcatagatgg tgacgttctt gctgcccttg ccgaagagcg cgccgatgcc
     1081 tgcgccgatg cctgcgccga cgagggcgat gaccgggacg atggagccga tgaactcgcc
     1141 gtcggccgag tcgtagacgc cggcgccgat ggcgaggccg cccccgccgc cgatggccgc
     1201 gccgaggagc gcgcccttcc cgcgcgacgt gccgcggttg atctggacga gacggacgcg
     1261 gtcgcgcgcg taggtgaccc gtcggccgtc gcgcgtgatg acgagctgga ggtcgttggc
     1321 gctgtcgaag cggccggtct tgcgcccgtc gtccgtcgtc ttgacgacca ctcgctcgcc
     1381 ggccgtgagc gcctgcacgg cccgccagtc gctcgtccgc gccgtcgcgt cctgcgccgc
     1441 ggcgggagtg attccggcca gcgccaccgt gagcgcgagg ccgagtgcaa acattctagc
     1501 tctcatgtta gtctccttcg ggccaaatgt aggccgacaa attaattccg agcgggaaga
     1561 gtgcaagact cattccgcac gcgcgacgtt gggaaatcgc ggccgcgagc gtcgaaagag
     1621 ttgacggccg cggccgggcg gcccgcagta aacgtccgac aggcgccggg cgcaggttgc
     1681 ccggtaagga ccgagcgcgg gcgcggccgg cgagcgaggg aaacgaacgg aggacaaaga
     1741 gcgtggcact ggttggcgcg aacgagctga agcggaagat gttgattgag gtcgagggcc
     1801 agcccttcgc ggtcgtcgag gtcttccacg cctcgcccac ggcgcgcggg gcgacgacga
     1861 tggttcgcac gcgtcttcgc aacctgctga cgggcgcggt gctggagaag agcttccgct
     1921 cgggcgagaa gttcgcggag ccggacgtgg cgctggctcc cgcctcgttt ctctactccg
     1981 acgccgaggg ctttcacttc atggacgagg agacctacga gcagctcatg ctcacgactg
     2041 aggccgtcgg cgacgaccgc ggctacctga aggatggcct gcgcctgcaa atcctcaagt
     2101 acaacggcga ggccgtctcc atcgaactcc cgcagtacgt cgagctggcg gtcgtccgca
     2161 cggagccggg cgtgagcggc gaccgcgccg caggtcgcga cctcaagccc gcgacgctgg
     2221 agaccggcat cgatgtgcgc gtcccgctct tcatcaagga gggcgagacc gtgcgcgtca
     2281 acacgcagac gggcgaggtc tcgggccgcg cctgacccac gtttgcgggc cgcgcctgac
     2341 acgctttcgc gccgtctgcg cttgactcgg gcggacgcgc cgtcgcataa cagacaagtc
     2401 ctcgcactcg ttacattcgt tccgccttcc tcgcggcggc caatcgaccc tcaagccggg
     2461 gccgtctgtc ctgacagcct ttcaaggagt ccgctcgcca tgacctcatc aaaaaaccgc
     2521 gccctcatcg cctcactcat cacctgcctg ctgctgctcg ccccgtggcc cacggcacgg
     2581 gccgccgact tcaagtcgga ggtcatctac cagatcatca ccgaccgctt cttcgacggc
     2641 agcgccacga acaacaaccc cgcgcagtcc gccggccttt acgacgcgac caagacgaac
     2701 tggcgcgctt actggggcgg cgaccttcag ggcatccagc aaaagatgtc ttacctcgcc
     2761 ggcatgggcg tcaccgccat ctggatttcg ccgcccgtcg ataacctcaa cgccaacatc
     2821 cccgacggca gcggcaaccc gacggcctcc tatcacggct atcagggccg cgacttcaag
     2881 cgcatcgaag agcactcagg cgagcagtcg aacgcatgga cagctttcga caatctcgtc
     2941 accgccgccc accagaacgg catcaaggtc atcgtcgatt tcgcccccaa ccactcgacg
     3001 caggacaacg ccggcgagtt cggcgccatt tacgacaacg gcgccttcct cggcaactac
     3061 accaacgacg tgagcggctt cttccaccac aacggcaaca tcgtcaattg ggacgaccgc
     3121 tatcaggtgc agtactacac gctcttcaac ctcgccgaca tcaatcaggc gaacccgacg
     3181 atggacgcct acatgaaggc gtcggcccaa ctcttccagc agcacggcgt ggacggcttc
     3241 cgcatcgacg ccatcaaaca catcaactgg ggctggcagt attctctggc gaactccatc
     3301 tacacctacg gcgacagctt cctcttcggc gagtggtatc agaccggcac tggcgacccg
     3361 ctctaccgcg actcctacaa gtacgccaac cgaagcggca tctcgatgct cgacttcccg
     3421 ctcaacaccg ccatccgcaa cgtcttcggc tcgaacgcca acttctccga gatcgacacg
     3481 gtgctcacgg ccgaggcggc gaatttcacg tgggacgaag acctcgtcac cttcatcgac
     3541 aaccacgaca tgactcgctt cctctcctta aacaacaacc agacgcggct ccatcaggcg
     3601 ctcgccttca ttctcacgca gcgcggcgtc ccctgcatct actatggcac cgagcagtac
     3661 ctcttcaaca acacttcggg cggcggcgac ccctacaacc gcccgatgat gaacgccttc
     3721 aacaccaaca ccacggccta cactttaacg aaccgcctct ccacactccg gcgcaacaac
     3781 cgggccgtcc cctacggctc gcaccagcag cgctggatga actcggacgt ctacatctac
     3841 gagcggaagt tctacggcaa cgtcgtcctc gtcgccgtca acaagggcgc ggcggccgtg
     3901 aacgtctcgg gcctcaacac ggctcttccc gtcggcgctt acaacgacta cctgacggga
     3961 ctgctctccg gcgtccaaat ctccgtcgcc tccggcacgg gcggcaacaa tcccgtcacc
     4021 accttcagtc ttccggcgaa ctccgtcgcc gtctggcagg cgaccgagac gggcggctcc
     4081 ccacaggtcg gctccatcgg ccccacggcc gcgcagcccg gcgtccgtgt caccatcggc
     4141 ggcaagaact tcggctcgac gcagggaacg gtgaagttcg ggacgacgac ggccacggtc
     4201 aactcgtggt cggcgacgaa gattgtcgcc accgtcccgg ccgtgactca gggcaactac
     4261 aacgtgacgg tgacgaactc cgcgagtcag gtctcgaacg gcatccagtt cacggtctat
     4321 caggacaagc tcatccccgt caacttcacc gtcttcaacg cctcgccgac gcaagtaggc
     4381 gactacatct tcctcaccgg caacacggtg gagttaagca actgggcaac cacatgggac
     4441 gccgcggtcg ggccgatgct cacgcccaac tacccgaact ggttcctcac ggcgagcgtc
     4501 cccgccggca agaccatcca gttcaagttc atcaagattg ccgcgggcgg cgcggtcaca
     4561 tgggaggccg gcgccaacca cagctacacc gtgcccacga gcggcaccgg gtttgttaac
     4621 gtcaactggc agtactgacg gggaattaac atggatggac aggacgaaca ggataaaaat
     4681 cagaggcgga tttgagattt gaaatttcaa atcctttatc ctgcccgtcc tatccatcca
     4741 tgttaaattt gcctcttccc agagtggagt gatgagacat cattcggtgg tggggcttgt
     4801 gctcgtgctg ctttctgcgg tggcgtgtgc ggcgcagacg caggccgccg gcgctcaggc
     4861 cgaagggaag ccgaagcccg accccgaggc gctgcggaag tacgcgggcc gctacgagct
     4921 ggaggtcggc ctcatccccg tctccaccct cgacgtgacg ctcgaaggcg gcgagctgtt
     4981 cgtcaaaccc tcgctcgtca ggaagcgcaa gctgatccgc aagtcgaaga acgtctttcg
     5041 agacgaggtc gaaggcgcgc gctacaggtt ccagacggac gacgagggcc aggtcgtcgg
     5101 cctcacgttt ccctacgaag gggccgagta cacggccgcg cgagtccgac tcccgccgcc
     5161 gagcacgagt ggcaacacga ccttccggct caagggccac cccggcgcgc aggtcgtggc
     5221 gctggccggc gccttcaaca actggaacca gtcgcagttc ctcttcggcc gcgagggcga
     5281 cgagtgggtc tgccgcatcg atttggagcc gggccgctac ggctacaagt tcatcgtgga
     5341 tggcaactgg ctgctcgacc cctcgaaccc cgagaccgaa gaggacgcgg ccggcaacat
     5401 caacaacgtg ctcgaagtga agccgcgctg aagcgaaaaa gttcctcgcg cgcgcggccc
     5461 ctccggctac caaccccgcc gccactgccg tttgagattc gcgcccgtct gtcgtagtgt
     5521 gtatacgtcc caaccccaat ccgtctaaat cttcttacta aggactgaaa gtcagcgcaa
     5581 tgagcactca accgaacaaa gtcatctatt cgatggtcgg cgtcagcaag cactacgaca
     5641 agcgcgccgt cctcaaagac atttatctct cttacttcta cggcgcgaag atcggcgtcc
     5701 tcggcctcaa cggctccggc aaaagctcgc tcctgaaaat cctcgccggc gtcgataaga
     5761 gcttcaccgg cgagacggtg atctcgcccg gccacacggt cggctatctg gagcaggagc
     5821 cgcggctcga cgagtctaga acagtgcgcg agatcgtcga acagggcgtg caggagacgg
     5881 tcgatctcct cagggagtac gacgagatca acgcgcgctt cggcgaggaa gtgtctgacg
     5941 atgagatgtc gaagctgctg gagcggcagg gcgccgtgca ggagaagctc gatgccgtgg
     6001 acgcgtggga cctcgactcg cgtttggaga tggcgatgga cgccctgcgt tgcccgcccg
     6061 gcgacacaag cgtcagcgtg ctctccggcg gcgagaagcg ccgcgtggcg ctgtgccgcc
     6121 tgctcttgca gaagccggac atccttctgc tcgacgagcc gaccaatcac ctcgacgccg
     6181 agtcggtcgc gtggctcgaa ctacacttgc agcagtaccc cggcactgtc atcgccgtca
     6241 cgcacgaccg ctacttcctc gacaacgtcg ccgggtggat tctcgaactc gaccgcggcg
     6301 agggcatccc gtggaagggg aattattcct cctggctcga acagaaacag gagcgcctgc
     6361 gccgggagga gaaacaggag accgagcggc agaaaacttt ggagcgcgag ctggagtgga
     6421 tacgcatggc cccgaaggcc cgccgcgcca agggcaaggc gcgcatcaac tcctacgaat
     6481 cgctcctcaa ccagcagggc gaaggccgca ccagggagct tgagatttac atcccggcgg
     6541 ggccgcgtct cggtcaggtg gtgattgagg ccgacgcggt gtcgaaggca tacggcgaca
     6601 acctgctcgt cgagggcatg acgtttcgtc tgccgccggg cggcatcgtc ggcatcatcg
     6661 gcccgaacgg ggcgggcaag acgactttgt tccgcatggt gacggggcag gagcagcccg
     6721 acgcggggac gattcgcgtc ggcgagaccg tcgaactcgg ctacgtcgat cagtcgcgcg
     6781 attcgctcga cgcggacaag tcgatctggg aggaaatctc cggcggtcag gacgtggtca
     6841 aactcggcaa ccgcgaggtc aactcgcgcg cctacgtcgg ccgcttcaac ttctcgggct
     6901 ccgatcagca gaagaaggtc agccagctct cgggcggcga gcgcaatcgc gtccatctgg
     6961 ccaagatgct caagtcgggc gctaacgtcc tgctgctcga cgagccgacg aacgacctcg
     7021 acgtgaacac gatgcgcgcg ctcgaagagg cgctcgagaa cttcgccggc tgcgccgtcg
     7081 tcatcagcca cgaccgctgg ttcctcgacc gcatcgccac acacatcctc gccttcgagg
     7141 gcgacagccg cgccgtctgg ttcgagggca actactccga ctacgaagag gacaagcgcg
     7201 cccgcctcgg cgacgccgcc gcgcagccgc accgcatcaa gtaccggcag ttgacgcggg
     7261 gctgaacacc gtcaatgtga atcagggagg attacaaaat gaaagcgaca tggaacgggt
     7321 cagtgattgc cgagagcgac gacacagtag tggtcgaggg taatcaatac ttcccggccg
     7381 actcgatcac gcgcgagcac ttccgcgaga gcgacaccca cacgacctgc ccgtggaagg
     7441 gcgaggcgag ctactacgac gtggtcgtga atggtgagac gaacaaggac gccgcgtggt
     7501 actacccgga gccgaaggac gcggcgaagg agattaaaga tcacgtcgcc ttctggcgcg
     7561 gcgtgaaggt tgagtagggg aggggcatgg atgattttca gaaatcgttt gacgggcaga
     7621 cgcgcgcgcg ccgccatcct ctctctgttc ttcgtcgcgg ctctcggcgc gggcgtctcg
     7681 gcgcagcaga agaagcggcc cgttaagtct ccgaagccgg cggccgtcac gccggccgtg
     7741 cgggccgcgc tcgaaggcgt gcgtgctgac tccttgcgcg gccacctctc cttcatcgcc
     7801 tccgatctgc tcgaagggcg cgacacgccc tcgcgcggcc tcgacatcgc cgccgagtac
     7861 gtcgccgcgc agttccgccg cgccggcctc gaacccgcag gcgatgacgg ctacttccag
     7921 acggccacct tcgccttacg cagcgcgccc gacacgccca tcaaatcacg caacgtcgtc
     7981 ggcctgctgc gcggctccga ccccgtgttg aaagacacct acgtcatgct gagcgcgcac
     8041 tacgaccacg tcggcgtccg cgagcaggcg gaagggccgg ataagatttt caacggcgcg
     8101 aacgacgacg cgagcggcgc cgtcaccgtc atcgaactcg ctggcgcgct cgcgcagctc
     8161 cgcccgcgcc cccgccgcag catcctcttc gtcgccttca cgggcgagga gaaaggtctg
     8221 caaggctcgc gcttctacgg ccggcatccg ctcgtgccgc tggagaagac cgtcgcgcag
     8281 gtgaacctcg aacacatggg ccgcaccgac gactcggagg ggccgcaggt gggcgccttc
     8341 ggcgtcaccg gcttcgacta ctcgtctgtc ggcgcgacgc tccagagggc gagcgaggcc
     8401 gggggcgtgc gcgtctacaa acacccgacc aacagcgacg ccttcttccg acgcagcgac
     8461 aacgcggcgc tcgccgacct cggcgtcccc gcgcataccg tcagcgtctc ctacatcttc
     8521 cccgactacc acggggcggg cgaccactgg gagaagatcg actacgccaa catggagcgc
     8581 gtggcgcgcg cgctcggtct cgggctgctg gctctcgcca acgactcgca ggagccgcgc
     8641 tgggacgagc agaacccgaa gacggagcgg ttcgtgaagg cgtggagaga gcggcgcgcc
     8701 aagtgagcga tgcgggaagt ggacgcgggt cttcgtcgcg cccggcgccc ggcgcgcctc
     8761 cgtcgttgac tccggcgcgt ctcccgtcgt agagtttgcg ctctttcgtg actgggcggc
     8821 cggcgccggg gcgacgactg cctcgcaccg tctaagccgg ccgttgttaa ctgaactttg
     8881 actgcccatg tgcggcggtt gcgagcggcc tgcactgctc tcgaccgtca cgtctcatcc
     8941 gccgccgccg cacacctcat gaaaccgttc gccccgaccg acacgccgcc cggaaagact
     9001 tgtttccaac atccgaggag agcactacat gccgtccctg aaatcgcgcc gcctgctcac
     9061 gtccctcatc cttctcgcca ctgcctccgc cctcatgctg cgcgcgccgt cgcccgtcgc
     9121 tctggcgcat cacacgccga acccttcgag cgtaacggtg gcgggtagtc ttcagagtga
     9181 gctgggctgt tcgggcgact ggcaacctga gtgcgcggcg acgcacctcg gctacgactc
     9241 gaatgacgac gtgtggcaac gcgtgttcag cgtccccacg gggaattggg agtacaaggc
     9301 gccgctcaac gacaactggg gcgagaacta cggcctccac gcgcagcaga acggcgccaa
     9361 catcccgctc acgctcgcct ctccgcagag cgtcaagttc tactacgacc acaagacgca
     9421 ctggatcacc gacaacacaa ctgcggtcat cgccgtcgcc cccggcagct tccagtcaga
     9481 actcggctgc tccggcgact gggacccgtc ctgccttcgc tcctggctgc aagaccccga
     9541 cggcgacggc atctacggct tccagaccac ggcgctcccg gccggcaact acgaggccaa
     9601 agtcgccatc aatgagagct gggacgagaa ctacgggcag ggcggcgtgc ccggcggcgc
     9661 caacatcgct ttcaacgtcc ccgcgaacaa cacgcctgtt acatttacct acaacgcaac
     9721 gacgcacgtc ctgaccatcg actcgggcag cgcgccgtcc ggcgacgtgc agtgggacgg
     9781 cctgcgccac gacaccttcg acccctacta ccgcagcccc ttcggcgccg tgcccgccgg
     9841 ctccaatgtc acgctccgct tccgcacggc ccacatggac gtgagcggcg tcgccgtgcg
     9901 cgtctatacc ttcgaccccg cgaccggcgc gaccgccggc cccgccgacc acccgatgac
     9961 gttccttgag aaccgcgtcg agggcggcac gacctacgac atctgggcgc tcacgctcac
    10021 gacgcccgtc agcccggccg tcctctacta caagttccgc gtcaacgacc agtcggacga
    10081 ggacttctac agcgactcct acacggacga ccacgacaac ctgaaccagg gtggcgaggg
    10141 cgcagccttc gacggcgagc cgttcccggc cttccagatc acggcctacg accccgcctt
    10201 ccagacgccc gcctggctcc acgacgccaa cgtctaccag attttccccg accgcttccg
    10261 caacggcgac gcggccaacg actactgccg ccccggctcc tcggccggct gccccacctt
    10321 ctacggcgac cagacgccgc tgctccgcga gccttggaac accgccatcg gcgacccgcg
    10381 cgcggccggc cccttccaga accagtacgg cacgcaattc ttcggcggcg acctcaaagg
    10441 cattgagaac aagctcgact atctccaggg cttaggcgtg gacaccgtct atctgacacc
    10501 catcttcaag gcgcgctcca atcaccgcta cgacaccgac gacttcctcg aagtcgatcc
    10561 cgcgctcggc ggcgacgcgg ccctcgcctc gctgcgcgcc gaggccgaac ggcgcggcgt
    10621 gcgccttatc ctcgacggcg tcttcaatca cacgtcgtcc gacagtcgat acttcgaccg
    10681 ctaccaccgc tacagcccgc cgcccgacgg cggttgcgag tcggccgcct cgcccttccg
    10741 cggctggtat aacttcttcg gcgtcggccc ctgcgacggc aacaactacg aggcgtggtt
    10801 cggcttcgac tccctgcccg tcctgcgcga cgactcggca agcgtgcgcg acttcttctt
    10861 ccgcgcgccc gcaacgaacg tcaccgagta ctggtacgcg cgcggcgtct cgggctggcg
    10921 cttcgacgtg gccgacaaca tctcgcacaa ctggtggcgc gagtaccggc cctatgccaa
    10981 gacctacaag gctgatgggc cgctcgtcgg cgaaatctgg cccgacgcca gtcagtacct
    11041 cgccggcgaa cagttggact cggtgatgaa ctaccgcttc cgcaagaacg tccttgggtt
    11101 cgcgcgcggc gcgggctgga atgataacga caacagcggc ggtaaccaga tcgccggcct
    11161 cgccccgagc cagttcgacc gcgccctgcg ctccgtgcgc gaggactacc ccgagccggc
    11221 cacccgcgcc atgctcaacc tcctcgactc gcacgacacc aaccgcgccc tctacgtcct
    11281 caccctgctc ggcgacagcg ggctcgcgca ggcgaaggag cgtctcaagc tctcggctct
    11341 cttccagttc acctaccccg gcgcgccgat ggtctactac ggcgacgagg gcgcgctcga
    11401 ctcgcccgcg ctcgcgctcg gccccaacgg ccccgaggac gacccataca accgcgcccc
    11461 ttacccctgg gccgacgagg ccggcgacac aaacgtctac ggccccgtcg atacgggcgt
    11521 gctcgacttc tacacgaagc tcgggcacat gcgtaagcag ttcccggccc tgcgcacggg
    11581 ctccttcgag acgctgctca cgggcgacac ctcggcctcg gccgcggaca attcgaccta
    11641 cgccttcgcc cgcgtgagcg gcgcgagccg cgccgtcgtg gcgctgaaca acggcgccgc
    11701 cgccaacacc gccagcctgc ccgtcgccgc acacttcgcc gacggcgccg agcttcagga
    11761 cgttctcggc ggcgccacct acacggtctc gggcggcgcc gtcaccgtca cgctcgccgc
    11821 gcgcggcggg gtcgtcctgc tgccggcccc ggccgtcatc gacacgacgg cccccgccgc
    11881 cgacatccag acgagccccg cccctaacgc caacggctgg cacgacgcca ctcccgtcac
    11941 cgtcaacttg agcgccacgg acgccgggag cggcgtcaaa gagctgcgct actgggtgaa
    12001 cggcggcgcc gtcacggtcg cgccgggcgg aggcgcgagc ctcggcctca cacaacaggg
    12061 cgtctacacc gtctcgctgc gggccatcga caacgcgggc aacgtgagcg cgctcgccgc
    12121 cctcgtcgtc cgcatcgaca cgaccgcgcc cacggtcacc gtcgccacgc cgcccggagc
    12181 ctcggccgac ggcaactgcc aagcggccat ccccaacctg acgccttaca cgacggcctc
    12241 cgacaacctg acgccggccg gctcgctcgt cctcacgcag aacccggcgg ccggcacgct
    12301 cgtcggcctc ggcgcacaca ccgtcaccgt gacggccacc gacctcgccg gtcacagcac
    12361 gtcggcctcg acgaccttca ccgtctctga caacacgccg cccgccgtca cgcagccgtc
    12421 ggccagcccg tccgcactca ctttgccgaa tcacaagatg gttgaggtga cgctcggcta
    12481 cggcgcgtcg gacaactgcg ctccggcgac gtgtgcgctg agcgtcgcga gcaacgagcc
    12541 tatcaacggc ggggacgacg gcaacacggc gcccgactgg gaagtcatca gcccgaccct
    12601 tgtgcgtctg cgggccgagc gctccggctc cggctcgggc cgcgtctaca ccatcaccgt
    12661 cacctgcacc gacccggccg gcaacccgac cgtgcgcagc gtgacggtga ccgttccgaa
    12721 ataacgccgc gcgccatttt cggcagacac gacgagaggc cccgacggaa acgtcggggc
    12781 ctctcacttt ctgcggcttc ctggcaacgg gcgtgcgcgg gtgtgcggct cgaaattatc
    12841 ccgaggggcg cacacgtaga gctgaccgac cgggggggcg gcgggcttcc ggggagaagc
    12901 ggcttgtgcg ggaattttcg gggcggtcag tgtccggcgg cggcgccggc cgcgacgcgt
    12961 gcgcgcgtcg gggtgcggcg ctcgtgccgt gtgcaggcgc gggccagcgc ctgctcgacg
    13021 cgctgcgtga cgttacattc gggctgtcgg cagacggccg ccagctccga gatgacgagg
    13081 taacgcgcgc gctccagcat ccgctgctcg cggaaagaga gcggcttgag gtggctcagg
    13141 tacgttagat tcttgaggac cccggcgacc tcgaagatgc aaccggagcg catcttctcg
    13201 gtgaactcct tgaagcggtc cttccagtcg tgggcggccg gggcgaagtc gtcttccagt
    13261 gccttgacga gcatctcgca ctcggccgaa gagatcgggg agcgcaggcc gacctcgccg
    13321 gcgttggcga cggggacgag caccaccgag ttagtcgccg ccagacggag cgtgtagaag
    13381 ggcagcgtcg tcgggccgat ctgcttctgc tcgatctgct cgatgatgcc gatcccatgg
    13441 ttggggtata cgaccttctg gccgatcttg aaagccacca gcacacctcc tgtgaataca
    13501 aaaaactgaa ctgcgccaca cgggtcgggt ttaagagggt tcccttgggg ttcgcgcttc
    13561 tcgcggcgca gcgagccggt actgaaatct aacgaaagtg taacaagcac ccctcgcgag
    13621 cgtcaagctt caggattctt tgaggaaact gcgtgaatta atgtttcaaa attgcaacaa
    13681 cgaccggcga cggggccggc gattgccgct gaggggcaga ggttcaggcg gacgcttccg
    13741 tgtcgggcgc ggccgggagt tcgagcgtga aagtcgtgcc ggcgccgggg gcggagcgca
    13801 caagggtctc gccgccgtgg gcgcgcgcga gatgcttgac gatggcgaga cccaagccag
    13861 tgccgcccat cgcgcgcgag cgggcgcggt ctacgcggta gaagcgctcg aagatgcgtg
    13921 cgaggtgctc taccgagatg ccctcgcccg tgtcggagac gtggacgcgg tcgcgccccc
    13981 cggcgcgctc gtgcgcgaca gtgacgctgc cgccctcccg gttgaacttg atggcgttgt
    14041 ccacgaggtt cgtcagcatc tgctccagcc gccgcgggtc ggcctgcaca cggacgcctg
    14101 ccggcacctc gctgcgcagc tcgacgccgc gctcggccgc gcacgacgcc agcgccgtga
    14161 agcactcctg cacaagcgag gcgaggcgga ctgacacagg ctcgaccgtg acggtgccgg
    14221 cctcgatggc ggatagctca aggatgtcgt tgatgaggtc gcgcatccgc tcggagttgc
    14281 ggcggatgac ggagaggaag cggcgggtag tctcggggtc gtccgtcccg ccggcttcga
    14341 gcgtctcgat gaaagtgagg atggccgtca gaggcgtccg cagctcgtgt gagacgtttg
    14401 agagaaattc ctgccggacg cgctccagcc gctccagccg cgtgatgtcg aagaagacgc
    14461 cgacggcgcc gcgcacgtcg ctgccgaagc tcgcttcctt gtgccggagc ggcgcgacct
    14521 gaaggtcgta gacgcgccgg tcgccgtcct cgcccgtctc gatcttcacc tcgacgcgct
    14581 cgccgcgcgc cacggccccc gcgaacgccg agtagaccga ggggttgcgc gtcagctcgc
    14641 tcaggcggcg ggggacggcc ccccggccgg ggtcgaagat ttcgcgcgcc gagtcgttcg
    14701 aggccacgac gcgcatgacg ctgtcgatga cgatgacgcc ctcgcgcatg ctgtccatcg
    14761 tcgcctccaa cagcgccccg gccggcacct cgcccgccgc caccgcgccc gaaacggccg
    14821 gcgtcgcgac gcccggagcc accgcgtcac gcgcgcccgc gccgtttgat agctcgtcgc
    14881 gcgcggccgg ctgcgagact ttgcgggcga ggagcgtgcc ggcgagcagg ccggcgagga
    14941 ggaagcagaa ggaaaaggcg gccgtctgcg gactccgcag gtagagcgcg gcgcaggcgg
    15001 ccgcgagcgc gccgcacaga gtaaccgcaa gccacccgcg cgagttcatg acgtgaagtt
    15061 tagagagcga aacgcgaaag cggtagccgg cagccccttc gacagaacca ctcgcgttaa
    15121 agtttttgta ggggcagggc gagtcggcgg cccacaggcc acgaaagaaa tttacatgga
    15181 tgggcaggat gggcaggata aagaatttga aatctcaaat ctgaaatttg aaatctcaaa
    15241 tctgaaatca gaaatcagcc ctttcttcat cctacctatc ctgcccatcc atgttaattg
    15301 ttctgtaagc acgggccgcg aacttgccct gccccgacaa atttactgta cgtccgatga
    15361 ttccacgccg gccgcctctg acttccccgc gcagccgacg aagcggtagc cgacgccgac
    15421 cacggtttcg atggcgtcgc cgcagccgcc gagcttttgg cggaggcggc ggatgtgtac
    15481 gtcgagcgtg cgcgtgtcgc cgtagtactc gtagccccac acgttgtcga ggagctgctg
    15541 gcgcgtggcg acgcgcccgg cgccgcgcgc gagcgttgcg aggagcgaga actccttgcg
    15601 cgtgagcttg acggcctcgc cgtcgcaggc gacgcgcata tcgccgaagt ctatcgagag
    15661 gcgcgcgtcc tcgtacccgg ccgcggccga ctcgtcggcg cggcgcatca cggcgcgcac
    15721 tctggccatc agctcgcgca ccgagaaggg cttggagatg tagtcgtcgg cgccgaggtc
    15781 gagccccgcc acgcgctcgg cctcggaggt gcgcgccgtg agcatgatga tgggtgtgcg
    15841 gcgcgtggcc ggctcgcgcc gcaggcggcg gcacaactcc gtgccgctca tgccggggag
    15901 catcaggtcg aggatgacga gcgcgggcgg actcttctgg tcgagcgcaa gctggagccc
    15961 ctgctcgccc gtcagcgcca cgcgcgcgtc gagaccctcg cgctccaggt tgtagcgtaa
    16021 cccttcggcg atgtcggggt cgtcttcgac gatgaggaca ggtcgggaca taagtcaggt
    16081 gataagtgat aggtgataag tgatgagaaa gagataagag gcgaccgact gccttttctt
    16141 atcactcatc actcatcact tatcacggct ttcaatggct atgcttgatg atctcggcct
    16201 cggtcatgta gacggtcagc tcgcaaacgt ccgtgacgta gtcgccgacg cgctccaggt
    16261 gcttggcgac gaggaggagg cgggcggccc gcttggtggt cgaggggtct tcggtcatgg
    16321 tctcgatgag gccgtggaag acgcggttgt aagcctcgtc gatctcgtcg tcgcggtcga
    16381 tgatgcggcg cgcgtcggcg gcgtcgccgc gggtgaaggc gtcgagggcg gagcggagca
    16441 tctgcgaggc ggtcgtggcc atgcgcggca ggtcggggag ggggcgaagc tccggctcgt
    16501 cgttgagatc gatggcggcg cgggcgatgt tgcaggcgtg gtcggcgatg cgctccagaa
    16561 tcggcgtgac cttggccacg gcgacgacga agcgcaggtc gcgcgccgcg ggttgcctga
    16621 gcgccagcac gtcgatgcag aggcggtcga tctcgacctc cagcagatcg atgatggtgt
    16681 cgccctctaa aacctcgcgc gccgcgccgc tgtcgcgctc cgagagcgcg tacatggcgc
    16741 gctggagcgc cgtctcggtc tcgccgccca agagcaacac gcggtcgcgc agaacgtcca
    16801 gctcctcgtc aagtcttctg tgtgtttcca tgattattag ctgtttgcga ttagcgatta
    16861 gctgttagct gttcggcatc gcccgcgacc ttattaagca cacaggctgg atgtaagacg
    16921 ttttagctaa cggctaatcg ctaacagcta actgctcctt tcacccgaag cgcccggtga
    16981 tgtagtcctc cgtcagcttc tgcacggggt tggtgaacat ccgctcggtc gggttcactt
    17041 cgatgagctt gccgagccag aagaaggccg tctggtcgga gacgcgcgcg gcctgctgca
    17101 tgttgtgcgt gacgatgacg atggtgtagt gccgcttcag ctcgaagatt agctcctcga
    17161 tcttctgcgt cgccatgggg tcgagcgccg aggccggctc gtccatcagg agcacttccg
    17221 gctcaacggc gagcgcccgc gcgatgcaca gccgctgctg ctgcccgccg gagagtccga
    17281 gcgccgactc tttcagacgg tctttaactt cgtcccacag cgccgcgtct tcgagcgcct
    17341 tgcgaacacg ctcctcagtc tcccgccgag tgctgccggc catccggttg atgcggagac
    17401 cgtaggcgac gttgtcgtag atcgacttcg ggaacggcgt ggacttctgg aagaccatcc
    17461 cgacccttcg gcgcagggag accacgtccg tgcccggcgc gtagatgtcg cggccgtcga
    17521 tgagcacctg cccctcgacg cgcgtgccga ggatgatgtc gttcatccgg ttgagcgtgc
    17581 ggagcacggt tgacttgccg cagcccgacg ggccgatgag ggccatcacc acgcgctcgg
    17641 gcacgtcgag cgacacgtcg aagacggcct gattcttcgc gtagaagaag ttcaggtggc
    17701 ggacgctgat cttggccgcc gccgccgcag tcgccgactc gccgcgtccc gactgctcgg
    17761 cagtcgcgct cgcgctcgga gtctgtgttc tggtcgagcg tgcagacata ctctctagcc
    17821 gaagtgcccc gtgatgtact cttccgtcag cttcaccgac gggttctcga acatcttctt
    17881 cgtccccgcg acttcgacga gcttgcccag atacatgaac gccgtgaagt cggagacgcg
    17941 cgccgcctgc tgcatgttgt gcgtgacgat gataatcgtc acctgccgct tcagctcgaa
    18001 gatgagttct tcgatgcggg aggtggcgat ggggtcgagc gccgaagtcg gctcgtcaaa
    18061 caaaagcatc tcggggtcgg tagcaagagc gcgtgcgatg cacagccgct gctgttgtcc
    18121 gcccgagagc gagtaggccg actccttcag cctgtccttg acctcgtccc acaacacggc
    18181 cgagcgcagc gcctgctcga ccttctcctc gacggcgcga cccttttcgc ccatcagccg
    18241 caacccgaac gccacgtttt cgaagaccga cttcgggaag gcgttcggct tctggaagac
    18301 catgccgatg cgcatccgca tctcgacggg gtcggtctcc ggcgcgatga tgttgacgcc
    18361 gctcggctgg agcgtgatgc ggccctcgta gcggttggcc ggatagagat cgtggatgcg
    18421 gttgaagcac cggagcagcg tcgatttgcc gcagcccgag gggccgatga tggccgtcac
    18481 gcggtgggcc gggaacgtca tgttaatgtc gttgagcgcc tgcttctgcc cgtagaagaa
    18541 gctcaagccc tgcacgtcgg ccttgacctc ggag
//
LOCUS       DASVES010000492        29467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_877144, whole genome shotgun sequence.
ACCESSION   DASVES010000492 DASVES010000000
VERSION     DASVES010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 29467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29467
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877144"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /gene="crtI"
                     /locus_tag="VF240_20650"
     CDS             <1..1568
                     /gene="crtI"
                     /locus_tag="VF240_20650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX7177681.1"
                     /translation="EIRGAAAEQILAHPSGVPVEMNPGANKKVLIVGGGLGGLSAGIH
                     LRLAGFDVTLLEAGERVGGRANLIERDGFRFDTGPSLLNYPWVFEQLFAAAGRDLRDY
                     VRLLSVDPSITFRWADGTRFTLSSDLQKFLAECERLEPGCASSVFEFLRDAGAKYRVA
                     FDKLVSRNVDNPLKWLAALSPSELRGTGLWRSFDGELRRHFKSRHIREALGSYAMYLG
                     GSPFNLPGMFTILPYGELAYGLWLPEGGVYGLVEGMEKLARELGVEIRTRSRVSKIRT
                     RGGRTHGVELADGTELEASLVVSNVDVPTTDTELVESENGFAARARARAAKTRMTPGV
                     VTFYWGVRGRVEGLGHHTIFLPDDYRAAFDDLMKHGRVPRDLPFYVSVPSATDPKLAP
                     PGCTAIFVLAPTPTLSQMPGADWHAESLKIKERVTETLSLHGVRIEPSRVVCEEVYTP
                     EDWRRRFGLYDGSAFGAAHTLFQVGPLRRRNFSEDVKGLYYVGASTTPGTGMPMVVLS
                     GKLVAERIVSHVR"
     gene            1558..2337
                     /locus_tag="VF240_20655"
     CDS             1558..2337
                     /locus_tag="VF240_20655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7177682.1"
                     /translation="MFVERHGRGGEVYFALHGWGGGRRTFAPLAPFVPDTASLYSADL
                     PGCGDSPAPREWSVDAVVGEIVETVRTLAPARVNFVGNCGGAVFALLAAQALGDTVGR
                     VVMIDPFAYLPRYFRLFIAGDFGRHAYNATFANPVGRWLTNQSLRGRRDNQSDLTVSF
                     ADADHEVARRYLVLFAEVGGVEGFRGFDVPVEIAHGEKTFGAVRRSLALWREVLPRVR
                     VRELKGAGHLPIEEATAEVAEVVFGSEGLSNGSRERKRVEV"
     gene            2334..3290
                     /locus_tag="VF240_20660"
     CDS             2334..3290
                     /locus_tag="VF240_20660"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7177683.1"
                     /translation="MSKQLAKFVAGGRGEVEAALRRSLPVSSLPGARRLNDALEYAVF
                     PGGKRLRPALALLASGLAGAAREQGLSVACAVEFLHSSSLILDDLPSMDDASFRRNRR
                     ALHLVYGEGVAVLASVALLNQSYALLAEAARVCCGREGAGTALVREAARCVGSDGMVG
                     GQVVDLETRAGAPGEDALACRDLKTVALMRLMMTSGALACGAPDEDARALADFGECFG
                     RAYQICDDLLDETRPSELTGKPARQDARHLRPNAVASLGAGGARRFAAQLIARGVSRL
                     SEQFGAREEVNLLADAAYQLLDATAEPCASRAGTQARAKAIA"
     gene            3290..4447
                     /locus_tag="VF240_20665"
     CDS             3290..4447
                     /locus_tag="VF240_20665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177684.1"
                     /translation="MPFVFSVAAVIIMLVACALLCMALWNALAWPRIGDRRDAASPTS
                     TPRSVSILIPARDEEANIAACLDAALAQGPCVREVLVYDDHSKDRTAQLVNEYARRDA
                     RVRLVAPAPLPAGWCGKTFACARLAEEARGRWLLFLDADARLDENAAALIVREAEARA
                     VSLLSCWPRLEMRGFWEGALMPLLNFVVFTLYPAPLALRRRNDPSLGLAHGSCMLARR
                     DDYELVGGHTAVRGELFEDVRLAQVWRARGRPSLCLDGQRVVGVRMYRSLAEIWGGFQ
                     KNFFPAFRRARGFWAFLALHLFVFLLPFALLPLVFSATGGAWAVIVAAVCVVATRVVL
                     ALRFRHPWWSALLHPLGETILIALGVSSWLRCRTGRGVEWKGRRYRAGAQT"
     gene            4507..5007
                     /locus_tag="VF240_20670"
     CDS             4507..5007
                     /locus_tag="VF240_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177685.1"
                     /translation="MSVFVSALLILSSFAGLAFPQTPKVRAEDLRPLTGAQWTGSLTY
                     LDYRANKKVSIPSNLTVTQLAGDEMSWVFEYQYPDEPKANSKQTVTLSKDGTVIDGET
                     VIERTSLDGRMLRIVTEQTGKDNDKDALFRYTYLLGPSSFSLKKEVRPEGVAEFFERN
                     QYSWKR"
     gene            5004..6476
                     /gene="crtI"
                     /locus_tag="VF240_20675"
     CDS             5004..6476
                     /gene="crtI"
                     /locus_tag="VF240_20675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX7177686.1"
                     /translation="MSTTKRRAVVVGGGLGGLATAIRLAARGWRVTVCEQGATFGGKM
                     NSLEREGFRFDTGPSLITMPWVFEELFAAAGTSLAEHVALASVSPLADYVFADGTRFT
                     YTTQLPEWLSTVRQIEPRDANGFMRFMRLGARLYALSQETFLRRPPTAPPDLRALKAL
                     RHLPLRHAWGNYHATVAAHFRSPQLRQLYDRYPTYVGSSPYRSPATLAVIPYIEYAYG
                     GWHVAGGLYRIVESLVSIARGLGVALHTDARVQSIEHEGRRVRGVRLADGTRLEADVV
                     VMNGDASTTHALLGEEGGAAGAPTARSLSGFVMLVGLRRKLNTLAHHTVYFSSNYAAE
                     FSQLFDERRFPDDPTVYVSAPSRNDPQLAPANGETLFIMANAPADEHAWDDRQIETAR
                     ARVFERLSQGGFPDIASDTVVSDVWTPDRIARDYLSPGGAIYGTHSHGWRRAFLRPPN
                     REARIGGLYHVGGSSHPGGGTPTVLLSARITSELIGRYDS"
     gene            6473..7234
                     /locus_tag="VF240_20680"
     CDS             6473..7234
                     /locus_tag="VF240_20680"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX7177687.1"
                     /translation="MSAPTQPENILYSLPRWRRATLAAVVPVFILLWVGGVASHWLGR
                     ESTDQGWLASLFLLLAGLIVLLGARTRRDALALAAVAVLGFVVEAIGVRSGVPFGQYV
                     YTGALQPQMLGVPVVMGLAWMALVAFASDFTRRLRLSLWPAAAVAALFTTACDLVIDP
                     LAANQFGYWTWAREGIYYGIPFTNFVGWFVTSLFACRIIAPRQRPNFWAGFVGTAILL
                     FFALIALAHSLLPVALIGFALCAARIFLLRRFQSI"
     gene            7614..9578
                     /locus_tag="VF240_20685"
     CDS             7614..9578
                     /locus_tag="VF240_20685"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7177688.1"
                     /translation="MLRVSLNAGLFLTLLFALCAGILIADHTSVGAQHSQAPGNKTAP
                     DLRRRVNSAQADEMVSVVIQPRAGWGDELDSVLRTQGARGLRRYHRLSAAVVELPASA
                     VASLAAHSEVAYVSLNREVSSFGHVSQTSGADAVRSANGATVAGLDGTGISIAVLDSG
                     MDTTHKAFLDKSNNSRIILSRDFTGEGRTDDPFGHGTHVASIAAGNGRISNGQYTGVA
                     PNANLINLRVLNSQGTGSVAGLLEALDWVLVNHAAYGIRVVSMSLGGPAVDSYRHDPV
                     CRAVRRLVDAGVVVLAAAGNNGKDSQGQKVYGQVHSPGNEPSAITVGASNSFGTDARG
                     DDVVTSYSSRGPTRSFWADADGVRHYDNLIKPDLVAPGNKLVYAQALNNRLVANNPSL
                     DAGVSPVDGRKMMYLNGSSMATPVAAGAAAMLLQANPSLTPNLVKMLLMYTAQPLAGF
                     NMFEQGAGQLNVEGAVRLARLVRTDLTAATPLGDPLLTAPEAPAAQTTIAGHTFAWAR
                     GGILGHTYASGADLITKYQKVYGVGSVLGDGVIIGDGVIIGDTYLSVDQAMMSSGVII
                     GDHVMTSGGVIIGDGLPFLSVSLLLGDGVIIGDGVIIGDGVIIGDGVIIGDAGLRADA
                     TLQAQSATSNGDDTPSMVIELDSGVDYLDY"
     gene            9633..10535
                     /locus_tag="VF240_20690"
     CDS             9633..10535
                     /locus_tag="VF240_20690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7177689.1"
                     /translation="MSAPLASTVPPNDIRPAAEFTSNALTVQTLRAGDEPEVLTFLAR
                     RPLYTVVMAGFVSDNGLESSLNRGVFYGCRGPEGRLEGVALLGHATLMEARAPAVLDA
                     FGRFAQALPRPHVIMGEQQVVARFWRGYADAGAAPRLSCRELLFEQRRPVGGRAAVTD
                     LRLATPDDLNGVMEVQARMAFEECGVNPLRTDPVGFRERCRRRIEKGRVWVVSRCGRL
                     VFKADAIADTPEIIYLEGVHVRPDSRGEGLGLDCLSQLGRTLLAKSASICLVVKEGRT
                     EAQDFYRKAGYEFRCYYDTIYPAK"
     gene            10595..12568
                     /locus_tag="VF240_20695"
     CDS             10595..12568
                     /locus_tag="VF240_20695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177690.1"
                     /translation="MGEVNAKNRYMWAIIAGGAGVLAVSAYRLSPALLDVKFGVLAVI
                     TIVVSSRLSSKIPRLSSHITVSDTFIFLTMMLYGGEAAVLLAAVEGLCSSLRVNKRPF
                     TTLFNSAVMACATFLTVTTLRLLFGSIRELSHEGYTPLFVVAVCVMALAQYVFNSGLV
                     SVLAAYKIDRPFWHTWRNYYLWSSITYFAGASAAGIIAKLITEVGFYAVIITTPIVAI
                     IFFTYETYRNNIEASAAQTAQAERHVEELSLHLAEREQAEKERDRLLVREQAARAEAE
                     RANRMKDEFLATLSHELRTPLTSIVGWTDLLRSDKLDPATRIMALTTIERSALTQVQL
                     INELLDVSRIITGKLLLDIQEVDLATVAAAAVEVMRPAAVAKGITLVQSCAPGAELVS
                     GDAARLQQVVWNLVANAVKFTPSGGRVEVRVERSVTHVVLTVSDTGAGISPEFLPHVF
                     ERFRQADSTTTRTHGGLGLGLAIVRHLAEMHGGMVHAESPGENMGATFYVSLPPAAIE
                     TEGRNARRVRRQDGLAGAAAGRPILDGVRVLVIDDERDARQVISAVLTHSGAEVHVCD
                     SASDALKELEGWTPDVIMSDISMPGEDGYDLLRKVRSLPGERVRSIPAAALTAYARDE
                     DRERALAAGFQKHIAKPVRSAELVGLVADLARI"
     gene            12882..13421
                     /locus_tag="VF240_20700"
     CDS             12882..13421
                     /locus_tag="VF240_20700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7177691.1"
                     /translation="MDSNDFTTKADQQLVEKFMRTKDETTFRELYRRHTPYLYLLALR
                     LVGGAVSEAQDAVQDTWLRACKTLSGFAWRSSLRTWLAGILINRVREMNRESVRRNEE
                     ELPEEFSLPGPARASEHVDLEQSIARLPAGYRLVLVLHDVEGYTHEEIGARLEISAGT
                     SKSQLHHARKALRAAFQGK"
     gene            13431..14045
                     /locus_tag="VF240_20705"
     CDS             13431..14045
                     /locus_tag="VF240_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177692.1"
                     /translation="MSQEFEDELSAEERRALDALVREKTALPLLEERVVGALKRAELL
                     RPAKSIRGLRAPRVGLAVAASLLFFVLGTLAGARWGAGAPERKTGVGEFMLVLRSAAG
                     QSQPDAPDEVRRRVGEYGKWAGRLRQQGVRVDGEKLRREARILRAADGSAAVSEERSE
                     ASQDAIAGYFLIEAQNYEQAVKVAEGCPHLKYGGTIEIRQIDRF"
     gene            14083..14952
                     /locus_tag="VF240_20710"
     CDS             14083..14952
                     /locus_tag="VF240_20710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7177693.1"
                     /translation="MKVLAHLASVLLLVGTGGFVNNPPAAKAELNAGVSESFIRTFGV
                     KINVEDMEKALAFYNGKLGFEVEDRRGYPDEVKLASGGREKIILNRVKKLRPLVPNDA
                     RLTFTLQVNDLDQAIRRLKALGVEFAETEKRKEAVGDAISIRDPFGRPISMMHQTVVK
                     VEPFKEPRVYNFGFLVPDMGVGRDFYTNKLGFVVRSEKYLPLDLPLGHPDATFAFMLH
                     VRPGVRPVKSAYPEAAPFLTMVFETADLPQTLEGLRRKQVNVVAVKDSQQGRVVVLED
                     PFGNVSEIVEARK"
     gene            15528..18194
                     /locus_tag="VF240_20715"
     CDS             15528..18194
                     /locus_tag="VF240_20715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177694.1"
                     /translation="MRPLDSSTARPRPGGRRPRVAGKFLYAGDQKLYVRGVTYGTFRP
                     GPDGRERFDPQQVARDFAQMREAGANALRTYTVPPRWLLDAAHAHGLYVMVGLPWEQH
                     VAFLDLPGRARDIERRVREMVRECADHPAVLCYTIGNEIPSPVVRWLGAARVERFLRR
                     LYKAVKKEDAGALVTYVNYPSTEYLDLSFADFFCFNVYLESEERLDAYLARLHNLAGD
                     RPLVMAEIGLDSRRNGEGAQAETLAWQVRTCFAAGCAGAFVFAWTDEWWRGGHDIEDW
                     DFGLVTRDRRPKPALAAVARAFDEVPFAPGGAWPRVSVVVCSYNGERTIRDCLEGLAE
                     IDYPNFETIVVNDGSTDRTPRIAGEYDVRLISTHNRGLSAARNTGAEAANGEIIAYTD
                     DDARPDPHWLYYLAHSFRRYGFAAVGGPNLAPAGDGLVADCVANAPGGPVHVLLSDRV
                     AEHIPGCNMAFRREVVQEVGGFDPAYRVAGDDVDLCWRVQRAGGTLGFHPAALVWHHR
                     RNSVRTYWRQQWGYGKAEALLEEKWPEKYNAAGHLTWGGRLYGKGLTRALLFRRGRVY
                     QGTWGAGLFQSVYEPAAGTLRSLPLMPEWFLVVFTLAALASFGLVWTPMLAAAPLFAL
                     ALAAPIAQAVLSARRAQFTGAPETRGALLKLQLLTATLHLMQPVARLLGRLRWGLTPW
                     RRRGVGGFAAPRARLTSVWSETWRGSEERLRDVEATLRAGGATVLRGGDFDAWDLEVR
                     GGLFGGARALTAVEEHGAGRQLVRARSWPRVSPLWLAITLLFAALACAAAWEGAQTAA
                     LAFALSALFVAARIVLESGAAMATMLDAFVRPAAAERERHARAREVLKVTPAAPGTLA
                     AAPAALRAEAAVEVGRVPPSVSEVVAQFEAEG"
     gene            18298..19287
                     /locus_tag="VF240_20720"
     CDS             18298..19287
                     /locus_tag="VF240_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177695.1"
                     /translation="MSERLTYRLLCEDDLPGLLRLWEGAGWGVLTPEQWRAWFVEGPQ
                     GPSLVTVGVDERGEVAAQEMFAPARAVVGGREVRALRLSAPILREELRGRSLKNAEHP
                     IIGLLKVSMSAAAEQGFAVVYSLPEYAWLPVFRRLPSYGIKPFAEAVYACASLPFEAA
                     RSTPVRSAAAGLEARAVEEFGEEFEELWREAKSSFPIDCGVVRDKSWLAFRNSGRIAV
                     EVRAASDGRLVGYSATKRATGLLADLLAREPAELKLVVAATLCWLEAERVRSVPEGLT
                     HLKVMRTPALAPALDALGFEPSDYRFAFTCNSFDPSLTLEEIAPERWYVLPGD"
     gene            19298..20275
                     /locus_tag="VF240_20725"
     CDS             19298..20275
                     /locus_tag="VF240_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177696.1"
                     /translation="MVLAYRLVREDDFPEVQRLWKEEAGWGTFAEEMWRRYVVEAPFG
                     GVSGTVATDTDTGRIVGQFAFVPSLVSVDGREVRAFRPGAPVIARGLRFRSANPFSHP
                     IVAMYKFAVKALRERGDGLIYMVPDRRWARLFKMFPFLQTGSFPLWRLALPTPAPLPL
                     GEGFTSAPLREWDARVDRLWADWRKFHGCMVVRDSRTMPWKLGDSRHELVAVERGGEL
                     VGLSASRRRKGEEQWLVCDLLAADTGEALRATLAATANLAHAKALESAATEKPIRKMT
                     VLVTPPMEAAVRGLGFARDDYDFPLVVHVLDRSLKSDVHPSRWFVSAND"
     gene            20283..21269
                     /locus_tag="VF240_20730"
     CDS             20283..21269
                     /locus_tag="VF240_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177697.1"
                     /translation="MALKLEFYEVADLPGLEQLWREETDWGDEVLRRLHEWFMAAPFG
                     PPRIVVAHEAGSNEIVGQFRFMPTRVSVDGREVRATRPFGTIITDRMRAAVESTNPLE
                     QPAAAMYVRAVEELRARGDQLIYMVPDPRWVRMFRMFPFLQSGSFPLWSLPVPLAGGP
                     LPLGQGYTSGPLAAWDDGRTDRLWNAARRQYGCMLVRDAQTLRWKLANANHTVTAVER
                     GGELAALVASRAKGDRQWLLCDLLAADDGEALRAALAAAANVAHAEALARTGAQEIRK
                     VAVLATPSMEPVLRDLGFARDDYDFPLVVHVLDDTLRAEDFNPSRWYVSAND"
     gene            21309..22433
                     /locus_tag="VF240_20735"
     CDS             21309..22433
                     /locus_tag="VF240_20735"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX7177698.1"
                     /translation="MPKYSTEISHGLGQAEALARLKSMTEQAGRVSDLKGTWSENTFE
                     FSVTTQGIGLRGTLRVEADALKFDGRLPLIAMPFRNWIPRVLKKGLAQAGTSVREAAA
                     PSPAEADARVGAEPDAPAVLFLHIPKAGGQTLGEYVYNHCRDAEARAASVADTLDAGV
                     AYLNYGFLKEPGLVVPEHVRRLLGRRDLRAVIGHFCFGLHEHVARPHVYVTLLRDPVE
                     RVVSLYHYAKLHETMGLEEFVGSPPFKEVDNDQTRRLAGVDPPLGRLTRDALDAAKEN
                     LRSHFAVAGTIERFDETLALLRLKLGWTREVVSYPRNVNSKRPAVASFPRETIEAVRA
                     RNELDLELWQYASRLMDEAIADTGEKFADELERYGALKGA"
     gene            22430..23638
                     /locus_tag="VF240_20740"
     CDS             22430..23638
                     /locus_tag="VF240_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177699.1"
                     /translation="MSEKLRIVVGGYVGLLPAGGVTWDYVQYPAGFAALGHDVYYVED
                     TRLWPIYQPEGSDWGDASASVAHLRRVMDAFDLPSRWAYRDEASGRIFGMSEEKLREV
                     CRTADVFVNVSCSTYMREEYRRIPARALIDSDPMFTQIQYLSQQMFTPGEPTQRDLVD
                     AHNFHFTFGENIGQEDCSVPDCGIEWRPTRQPVCLQHWRATPPHSGAVNDEGAAYTTL
                     MNWTAARPLQHDGETWGQKDVEFRRFLHLPSLAPEVTLAVAVGQTGGTGEPFPAAEAR
                     AAGWRVLDPEVCAPEWRSYRDFIEASRGEFSVAKETYVKARTGWFSCRSACYLASGRA
                     VVTQDTGWSKFLSSSEGLLAFDTPEGAGDALRRVEADPARHSAAARAVAEEHFDSSRV
                     LSSLLAEMGG"
     gene            23669..23935
                     /locus_tag="VF240_20745"
     CDS             23669..23935
                     /locus_tag="VF240_20745"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7177700.1"
                     /translation="MPEAIEQRVMKAIAATAHAPIEQVTPETTFEELGMTSLDALALI
                     ADLEEEFRIEIPNEDALSLRDVRQAVACVRALLPEASGPEARPS"
     gene            23932..25152
                     /locus_tag="VF240_20750"
     CDS             23932..25152
                     /locus_tag="VF240_20750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7177701.1"
                     /translation="MSAPRRVVITGMGAVTALGRNVAEFWDALAAGRSGIRPLETVDR
                     SRLRFENGAEVRGYDPAGHFSDKELDLLDRFAQFGLVAAREAVKDAGVEWTRDLRERT
                     CVVTGSCLGGKISEDEAFGGFYAEGRRAHPLAIPRAMSNAAASHISMEFGILGPTYNV
                     STACASATHAIGQAFALVRSGAAELALTGGCEAPFALGHLKKWEALRVVAPDTCRPFS
                     KDRRGMILGEGGAMLVLEPLDAALARGARVHAEVVGFGMSADAHHVTQPLAEGAARAV
                     AAALRDADVRPEQVGYVNAHGTATAANDPAETEALRLVFREHAARLAVSSTKSMHGHA
                     LGAAGAIEAVATALALREKLLPPTANYSEPDPACDLDYIPNEARAAEVGYAVSNSFAF
                     GGLNGVLVLKRWDS"
     gene            25152..25745
                     /locus_tag="VF240_20755"
     CDS             25152..25745
                     /locus_tag="VF240_20755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7177702.1"
                     /translation="MTEARLFHPLTPGAELLGDWFAGRIPLNVVAGEGTVIDSSFCFK
                     HFYSAREVGLKVGGRTTIWRASLAADEEAVIEIGDECYIANASLVCSELISIGSRVMI
                     AGGVTIADSDFHPLGPAARLADTVALSPVGDRRRRPAVETRPVRIEDDVWIGYNAAVL
                     KGVRVGAGALVAPGAVVIRDVPPGAEVAGNPARVVCE"
     gene            25746..26414
                     /locus_tag="VF240_20760"
     CDS             25746..26414
                     /locus_tag="VF240_20760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7177703.1"
                     /translation="MKENIPTSLAPDVGARRHLEHDWYAGGIPANVRFAEGVYVDTSY
                     GFAAFHSECAVGLALGDAAGAYDRTTFVVGPAGQINVGAYTVLNGTYIVCNGRIDIGA
                     HCLVAWGSVLTDTWADAAALPVALRREVLRACAADPARRLPAFAEPRAVVLEDNVWVG
                     FDAVVLPGVRVGRGSVVGCKTVVAEDVPPYAVVVGSPARVVRRLDADDTEEERKRAVR
                     EYAR"
     gene            26424..27407
                     /locus_tag="VF240_20765"
     CDS             26424..27407
                     /locus_tag="VF240_20765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7177704.1"
                     /translation="MERRISIDLIVCTYNNAALLDRTLDALARQRVRPEVDWGVLVVN
                     NNCTDETPEVLEKHLRATPLPLRVVVESRQGLTPARVRGVRETASEWVAFVDDDCLLA
                     EDWVEQAARFAVEHPECGGFGGQVIPEWETPPPPYVLGNLYAYACKSHGERPHRRPWL
                     AGAGMVVRREALKSCGWLERQFLEDRKGSRLVSGGDMEIALRVAARYELWHNPACRLR
                     HFIPARRMSREYLRRVTRGLGASRHNAEALKWKGSYAGWLLYSSGLSLGFGALGLLRL
                     ARGLFSSEVDFTTAFSPAAGWCSAMWGMLRMDAAERGELIGCAAARFDRRG"
     gene            27419..28810
                     /locus_tag="VF240_20770"
     CDS             27419..28810
                     /locus_tag="VF240_20770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7177705.1"
                     /translation="MHASETEFVTTTTTRAATAREGAAPALTVALVDWSQLLEDYLDN
                     IGVSFESFRDEMTGGWMFGYVEALRLAGVRAVLFCVSARVTETERFTHAPTGAALRVL
                     PAPRAYRRLRRRVLNPYATTVEEAVGVVEGRARRACWSAARELMPYLSTPVVALAREL
                     RRERCDALLVQDYEHGRFDICLALGRLTGRRVYATFQGGDFSHGRLERVTRRRSLRQA
                     DGFVVAGRGEAERLRERYGLTDEKIARVFNPLDLSEWRPGDRAAARAALGIPESARVV
                     VWHGRVDFRRKGLDVLLEAWRRVCFERPDGDLRLFLVGAGNDTEELRAAVSKASAEGV
                     RGLVRVDEYVTGRGRLRAYLRAADVYAFPSRHEGFAVAPLEAMACGLPVVAADAPGVP
                     DLLEGGEDSGGILVPRGDASAFAAAIVRLLDDEALRLALGARARARVAGEFALEAVGL
                     RLRDFMLKGGCQD"
     gene            28798..>29467
                     /locus_tag="VF240_20775"
     CDS             28798..>29467
                     /locus_tag="VF240_20775"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX7177706.1"
                     /translation="MPRLTRLGANGKVFGIGLSKTGTSSLTDALNLLGIPAVHYPHDE
                     RTLCELRAGRYRLSLLKEFQGVTDIPIAPFYAQLDRAFPGSRFILTVREREAWLRSCE
                     VHWRLMDEWLDNFPYFKRFHEFACLAAYGTLQFGRERFARAYDEHERGVRDYFRERPG
                     DLLVLDICGGDRWEKLCALLGLPAPAVPFPHANEWMHLLLEASADVARTIPEGETFIL
                     VDEEG"
BASE COUNT         4527 a        10011 c         9961 g         4968 t
ORIGIN      
        1 gagaaattcg aggtgctgcc gccgagcaaa tactggcgca tccctctggc gtacctgtcg
       61 aaatgaatcc gggggcgaac aaaaaagtcc tgatcgtggg cgggggccta ggcggcctga
      121 gcgccgggat acacctgcgc ctcgccggct tcgacgtgac gctgctcgaa gccggcgagc
      181 gcgtgggcgg tcgcgccaac ctcatcgagc gcgatggctt ccgcttcgac acggggccgt
      241 cgctgctcaa ctacccgtgg gtcttcgagc aactcttcgc cgccgccggg cgcgatctgc
      301 gcgactacgt gcggctgctc agcgtcgatc cctcgatcac tttccgctgg gccgacggca
      361 cgcgcttcac gctttcgagc gacctgcaaa agtttctcgc cgagtgcgag cgtcttgagc
      421 cgggttgcgc gtcttcggtc ttcgagttcc tgcgcgacgc gggggcgaaa taccgcgtcg
      481 ccttcgacaa gctggtgagc cgcaacgtgg acaacccgtt gaagtggctc gccgcgctct
      541 cgccctcgga gctgcgcggc acaggtctgt ggcgctcgtt cgacggcgag ttgcgtcgcc
      601 acttcaagag ccgccacatc cgcgaggcgc tcggctccta cgcgatgtac ctcggcggct
      661 cgcctttcaa cctgccgggc atgttcacca tcctgcccta cggcgaactc gcctacggcc
      721 tctggctccc agagggcggc gtctatgggc tggtcgaggg gatggaaaaa ctcgcgcgcg
      781 aactcggcgt cgaaattcgc acgcgctcgc gtgtgtcgaa gatcaggacg cgcggcggtc
      841 gcacgcacgg cgtcgagcta gcagacggca cggagttgga agcgtctctg gtcgtctcga
      901 acgttgacgt gcccacgacc gacacggaac tcgtcgagtc ggagaacggt ttcgcggccc
      961 gcgcgcgcgc gagggccgcg aagacgcgga tgacgccggg cgtcgtcacc ttctactggg
     1021 gcgtgcgcgg ccgtgtcgaa gggcttggtc atcacaccat ctttctgccc gacgactacc
     1081 gcgcggcgtt cgacgacctg atgaaacacg gtcgtgtccc gcgcgatcta cccttttatg
     1141 tgagcgtccc ctcggcgacc gacccgaaac ttgcgccgcc cggctgtacg gccatcttcg
     1201 tgctcgcgcc gacgcccacg ctctcgcaga tgcccggcgc ggactggcac gccgagtcgc
     1261 tgaagataaa agagcgcgtg acggagacgc tgtcgctgca cggcgtccgc atcgagccgt
     1321 cgcgcgtcgt gtgcgaagag gtctacacgc cggaggactg gcggcggcgc ttcggcctct
     1381 acgacgggtc ggccttcggc gcggcgcaca cgctctttca ggtcgggccg ctgcgccgca
     1441 ggaatttttc cgaagacgta aaggggcttt actacgtcgg cgcgagcacg acgccgggga
     1501 cggggatgcc gatggtcgtg ctctctggaa agctcgtcgc ggagaggatc gtcagccatg
     1561 ttcgttgagc gtcacggacg gggcggcgaa gtctacttcg cgttgcacgg ctggggcggc
     1621 ggccggcgca cgttcgcgcc gctcgcgccg ttcgtccccg acacagccag cctttacagc
     1681 gcagacctgc cgggctgcgg cgactcgccc gcgccgcgcg agtggtcggt tgatgcagtg
     1741 gtcggcgaga tcgtcgagac ggtgcggacg cttgcgccgg cgcgcgtgaa tttcgtcggc
     1801 aactgcggcg gcgcggtctt cgcgctgctg gcggcgcagg ctttgggcga cacggtcggg
     1861 cgcgtggtga tgatcgaccc gttcgcgtat ctgccgcgtt acttccggct gttcatcgcc
     1921 ggcgacttcg ggcggcacgc ctacaacgcg acgttcgcca accccgtcgg gcgctggctg
     1981 acgaaccagt cgctgcgcgg ccggcgcgat aatcagagcg atctgaccgt ctcgttcgcc
     2041 gacgcggatc acgaagtcgc gcgccgctac ctcgtgctct tcgcggaggt gggcggcgtt
     2101 gaaggcttcc ggggcttcga cgttcccgtc gagatcgccc acggcgagaa gaccttcggc
     2161 gcggttcgca ggtcgctcgc gctctggcgc gaagttctgc cgcgcgtgcg cgtgcgcgag
     2221 ctaaagggcg cggggcattt gccgattgaa gaggcgaccg ctgaggtggc tgaggtggtc
     2281 tttgggagcg agggcctgtc taacggttcg agagaaagaa agcgggtaga ggtgtgagca
     2341 agcagttagc aaagtttgtc gcgggcgggc gcggcgaggt cgaggcggcg ttgcggcgca
     2401 gcctgccggt cagttctctg ccgggcgcgc ggcgcctcaa cgacgcgctc gaatacgccg
     2461 tcttccccgg cggcaagcgc ctgcgccccg cgctcgcgct gcttgcctcc ggtctcgcgg
     2521 gggcggcgcg cgagcaggga ctgtcggtgg cgtgcgccgt ggaatttctg cacagcagct
     2581 ctctcatcct cgacgaccta ccctcgatgg acgacgcgag ctttcgccgc aaccgacgcg
     2641 cgctccacct cgtctacggc gagggcgtgg cggtgctcgc atcggtcgct ctgctcaacc
     2701 agtcttacgc gctgctcgcg gaggcggcgc gcgtctgctg cggccgggaa ggggcgggca
     2761 cggcgctcgt tcgcgaggcc gcgcgctgcg tcgggtcgga cgggatggtc gggggacagg
     2821 tcgtagattt ggagacgcgc gcgggcgcac cgggcgagga tgcgctcgcg tgccgcgacc
     2881 tcaagaccgt ggcgctcatg cgtctgatga tgacctcggg cgcgctcgcc tgcggagcgc
     2941 ccgacgagga cgcgcgcgca ctcgccgact tcggcgagtg cttcgggcgc gcctaccaga
     3001 tttgcgacga cctgctcgac gagacgcgcc cgagcgaact gacgggcaag cccgcgcgac
     3061 aggacgcgcg ccacctgcgc ccgaacgccg tcgcgtcgct cggcgcgggc ggcgctcgac
     3121 gcttcgccgc tcagttgatc gcgcgcggcg tttcacggct ctctgaacag ttcggcgcgc
     3181 gcgaggaggt gaaccttctc gccgacgcgg cctaccaact gctcgacgcc acggctgaac
     3241 cgtgcgcttc cagagcgggg acgcaagcgc gcgcgaaagc aatcgcctga tgccctttgt
     3301 cttctctgtc gccgccgtca tcatcatgct cgtcgcctgc gcgctcctct gcatggcgct
     3361 ctggaacgcg ctcgcgtggc cgcgaatcgg cgaccgacgg gacgcggcgt cgccgacatc
     3421 aactccgcgc tccgtgtcga tactcatacc cgcgcgcgac gaagaggcca acatcgccgc
     3481 ctgcctcgac gccgcgctcg cgcaagggcc gtgcgtgcgc gaggttctcg tctacgatga
     3541 ccactcgaag gatcggacgg cgcagctcgt aaacgagtac gcgcgacgcg acgcccgcgt
     3601 gcgcctcgtc gcgcccgcgc cgctgcccgc cggctggtgc gggaaaacct tcgcctgcgc
     3661 gcggctggcc gaagaggcgc gaggacgttg gctgctcttc ctcgacgcgg acgcgcggct
     3721 cgacgagaac gccgccgcgc tcatcgttcg cgaggccgag gcgcgcgcgg tctcgctgct
     3781 ctcgtgctgg ccgcgcctgg agatgcgcgg cttctgggag ggcgcgctga tgccgctcct
     3841 caacttcgtc gtcttcacgc tctaccccgc gccgctcgcg ctccgccgcc gcaacgatcc
     3901 gtcgctcgga ctcgcgcacg gctcgtgcat gctcgcgcgc cgcgacgact acgaactcgt
     3961 cggcgggcat acggccgtgc gcggcgaact cttcgaggac gtgcggctgg cgcaagtctg
     4021 gcgcgcacgc gggcggccga gtctttgtct cgacggccag cgagtcgtcg gcgtgcgaat
     4081 gtaccgctcg ctcgcggaaa tctggggcgg cttccagaaa aatttctttc cggccttccg
     4141 ccgcgcgcgg ggcttctggg cgttcctcgc gctgcacctc ttcgtgttcc tgcttccctt
     4201 cgcccttctg ccgctcgtgt tctcggcgac cggcggcgcg tgggctgtca ttgttgccgc
     4261 cgtgtgcgtc gtcgcgacgc gcgtcgtgct ggcgctcagg ttcagacacc cgtggtggtc
     4321 ggcgctgctg catccattgg gtgagactat cctgatcgct ttgggcgtct cgtcgtggct
     4381 gagatgcagg acggggcgcg gcgtcgagtg gaagggtcgc cgctaccgcg cgggcgcgca
     4441 gacttgatgc ccggcggcgc gcaaagaatt gttggatggc tacttttaaa ctgaaggagt
     4501 tagagaatga gcgtcttcgt ttccgccctg ttaatcttgt cgtctttcgc cggactcgcc
     4561 tttccgcaaa cgccgaaagt gcgcgccgaa gatttgcgcc cgctgacggg tgcgcagtgg
     4621 acggggtcac taacttatct ggactaccgc gcgaacaaga aagtctcgat accctcgaac
     4681 ctgaccgtga cgcagttggc gggcgacgag atgtcgtggg tcttcgagta tcagtacccg
     4741 gacgagccga aggcgaacag caaacagacc gtgacgctga gcaaggacgg gacggtcatc
     4801 gacggcgaga ccgtgatcga gaggacgagc ctcgacggcc gaatgctcag gatcgtgacg
     4861 gagcagaccg gaaaagataa cgacaaagac gcgctgttcc gctacacgta cctgctcggc
     4921 ccgtcgagct tctcgctcaa aaaggaagtc cgccccgaag gcgtggcgga gttcttcgag
     4981 aggaaccagt acagttggaa aaggtgagca cgacgaagcg gcgtgctgtc gtcgtcggcg
     5041 gtggactggg agggttagcc acggcgatca ggctcgccgc gcgcggctgg cgcgtgacgg
     5101 tctgcgagca gggcgcgacg ttcggcggga agatgaactc gcttgagcgc gagggctttc
     5161 gcttcgacac cggcccttcg ctcatcacga tgccgtgggt ctttgaagag ctgttcgcgg
     5221 cggcgggcac gtcgctcgcc gagcacgtcg cgctcgcgag cgtcagccct ctcgccgact
     5281 acgtcttcgc cgacggcacg cgcttcacct acaccaccca actccccgaa tggctctcga
     5341 ccgtgcggca gatcgagccg cgcgacgcca acggcttcat gcgcttcatg cgactcggcg
     5401 cgcgtctcta cgcgctctcg caggagacat tcctgcgacg cccgccgacc gcgccgcccg
     5461 acctgcgcgc gctcaaggcg ctcaggcatc tgccgctgcg tcacgcgtgg ggcaactacc
     5521 acgcgacggt cgccgcgcac ttccgcagcc cgcagctgcg ccagctctac gaccgctacc
     5581 cgacctacgt cggctcttcg ccctaccgct cgcccgcgac tctcgccgtc atcccctaca
     5641 tcgagtacgc ctatggcggc tggcacgtcg cgggcggact ctaccgcatc gtcgaaagtc
     5701 tcgtctcgat tgcgcgcggc ctcggcgtcg cactccacac ggacgcgcgt gtccaaagca
     5761 tcgaacacga gggccggcgc gtgcgcggcg tgcggctcgc cgacggaaca cggctcgaag
     5821 ccgacgtggt cgtgatgaat ggcgacgctt cgaccacaca cgctctgctc ggcgaagagg
     5881 gcggcgcggc cggggctccg acggcgcgct cactctccgg cttcgtgatg ctcgtcggtc
     5941 tcagacgcaa gctcaacact ctcgcgcatc acaccgtcta cttctcttcc aactacgcgg
     6001 ccgagttctc gcagctcttc gacgagcggc gcttccccga cgacccgacc gtctacgtca
     6061 gcgcgccgag ccgcaacgat ccgcaactcg cgcccgcgaa tggtgagacg ctcttcatca
     6121 tggctaacgc tcccgccgac gaacacgcgt gggatgaccg gcaaatcgag acggcgcgcg
     6181 cgcgcgtttt cgagcggctg tcgcagggag gctttcccga catcgcctcc gacacggtcg
     6241 tctccgacgt gtggacgccc gaccggatcg cgcgtgacta tctcagcccc ggcggcgcta
     6301 tctacggcac gcattcacac ggctggcggc gcgcgttcct gcgtccgccg aacagggagg
     6361 cgcgcatcgg cgggctttac cacgtcggcg gcagcagcca ccccggcggc ggcacgccca
     6421 ccgtcctgct ctcggcccgg attaccagcg aactcatcgg gaggtacgac tcttgagcgc
     6481 gccgacgcag ccggagaaca ttctttactc tctgccccgc tggcgacgtg cgacgctggc
     6541 ggccgtggtg cccgtcttca ttctgctgtg ggtcggcggc gtcgcgagcc attggctcgg
     6601 cagagagagc actgatcagg gctggctcgc ctcgctcttt cttctactcg cgggactgat
     6661 cgtgctgctc ggcgcacgca cgcgccggga cgcgttggcg ctcgccgcag tcgccgtgtt
     6721 gggcttcgtg gtcgaggcca tcggcgtgcg ctccggcgtc cccttcggac agtacgtcta
     6781 cacgggcgcg ctgcaaccgc aaatgctcgg cgtgccggtc gtcatgggcc tcgcctggat
     6841 ggcgctcgtc gctttcgctt ccgatttcac gcggcgctta cgcttgtccc tctggcccgc
     6901 cgcggcggtc gccgcgctct tcacgacggc gtgtgatctt gtcatcgatc cgctcgccgc
     6961 gaaccagttc ggatactgga cgtgggcgcg cgagggaatc tactacggca tccccttcac
     7021 caacttcgtc ggctggttcg tcaccagcct gtttgcctgc cgcatcattg cgccgcgcca
     7081 gcggccaaac ttctgggcgg gcttcgtcgg cacggccatc ctcctcttct tcgctttgat
     7141 tgcgctggcg cactccctgc tccccgtcgc cctcatcggc ttcgcgctgt gcgccgcccg
     7201 aatttttctc ctgagaagat tccagtcaat ataaaaagtg agtgccggaa ttcaaaagaa
     7261 ccggactccc cttcgccctt gtcagccacg cctaatagtt gagcctcagc ccgaactgga
     7321 aacagtttgg gccgattcaa aaccaatctc atctttgaat tttcacttac ctgacttact
     7381 gcgcaacacc cacgtgcaag actcgccttg ggggagtcgt gaattttttt accgaaggtc
     7441 gcgttaaacg ttgctgtctg accgaaagaa tgctaaaccc atgcttgcaa ttcgcagcgg
     7501 atgtttaaca cattcgttaa cccaccaatt ttgttcattt ggcttttccc acgaaccagc
     7561 ggcggcccgg agtcagatgc acatattttt cgttttgtga cagggggtga attatgctcc
     7621 gcgtgagcct caacgccggt ttgtttctga cactcctctt cgcgctttgc gcgggcatcc
     7681 tgatcgccga tcacacgtcc gtcggcgctc aacactctca ggcgccgggc aataaaaccg
     7741 cgcccgacct tcggcgacgt gtgaacagcg cgcaggcgga tgagatggtc agcgttgtca
     7801 ttcagccgag ggcgggatgg ggcgatgaac tcgactccgt cctgcggact cagggcgcgc
     7861 gcggcctgcg ccgctaccac cgcctgtcgg ccgcggtggt cgaactcccc gcgagcgccg
     7921 tcgcgagtct ggcggcccac agcgaagtgg cctacgtctc gcttaaccgg gaagtcagtt
     7981 ccttcggcca cgtctcacag acgagcggcg cggacgccgt gcgcagcgct aacggcgcga
     8041 cggtcgccgg cctcgacggc acagggataa gcatcgccgt gctcgactct gggatggaca
     8101 ccacccacaa ggccttcctc gataagagca acaactcgcg catcatcctc agccgcgact
     8161 tcacgggcga ggggcgcacc gacgacccct tcggccacgg cacccacgtc gcctccatcg
     8221 ccgccggcaa cgggcgcatc tcgaacggtc aatacacggg ggtcgcgccc aacgccaatc
     8281 tcatcaacct gcgtgtgctc aactcgcagg ggacgggcag cgtcgccgga ctgctggagg
     8341 cgctcgactg ggtgctggtt aaccacgccg catacggcat cagggtcgtc agcatgagcc
     8401 tcggcgggcc tgccgttgac tcctacaggc acgaccccgt ctgccgcgcc gtgcgccgcc
     8461 tggtggacgc cggcgtggtc gtcctcgcgg cagcgggtaa caacggcaag gacagccaag
     8521 gccagaaggt ctacggtcag gtccactcgc ccggcaacga gccttcggcg atcaccgtcg
     8581 gcgcgtcgaa ctcattcggc accgacgcgc ggggcgacga cgtcgtgacc agctacagct
     8641 cgcgcggccc cacgcgcagc ttctgggccg acgccgacgg cgtcaggcat tacgacaacc
     8701 tcatcaaacc ggacctcgtc gcccccggca acaaactggt ctacgcgcag gccctcaata
     8761 accgcctcgt cgccaacaac ccctcgctcg acgccggggt aagcccggtt gacggccgca
     8821 agatgatgta cctgaacggc tcgtcgatgg ccacgccggt cgcggccggc gccgcggcga
     8881 tgctcctgca agccaatccc tccctgacgc ccaatctcgt caagatgctt ttgatgtaca
     8941 ccgcgcagcc gctcgccggc ttcaacatgt tcgagcaggg cgcggggcag ttgaacgtcg
     9001 agggggccgt gcgtctggcc cgcctcgtcc gcaccgactt gacggcggcc acgcccctcg
     9061 gcgatccgct gctcaccgcc cccgaggccc ccgccgcgca gacgaccatc gccggccaca
     9121 ccttcgcgtg ggcgcgcggc ggcatcctcg gtcacactta tgcctccggc gcagacctca
     9181 tcaccaagta ccagaaggtg tacggcgtcg gttccgtgct cggcgacggc gtcatcatcg
     9241 gcgacggtgt gattatcggc gacacgtacc tgagcgttga tcaggcgatg atgtcgagtg
     9301 gcgtcatcat cggcgaccac gtgatgacga gcggcggcgt catcatcggc gacggcctgc
     9361 ccttcctctc cgtcagcctg ctgctcggcg acggcgtgat catcggcgac ggcgtcatca
     9421 tcggtgacgg cgtgattatc ggcgacggcg tgatcatcgg cgacgccggg ttgagggcgg
     9481 acgcgacctt gcaggcacag tccgcgacct ccaacgggga cgacaccccc tcgatggtca
     9541 tcgagttgga cagcggggtt gattacctcg actactgatg tcgtcggccg tttaggttga
     9601 cggtagagat tccccgatct gaggtgcggc tcatgtcagc cccgcttgct tccacagttc
     9661 caccgaacga catccgcccg gcggccgagt tcacctccaa cgcgctgacc gtccagacgc
     9721 tccgcgcggg cgacgagccg gaagtcctga ccttcctcgc ccgccgcccc ctctacaccg
     9781 tcgtcatggc cgggttcgtc tccgacaacg ggcttgagag ttcactcaac cgcggcgtct
     9841 tctacggctg ccgcgggccg gagggccgac tggagggcgt ggccctgtta ggccacgcca
     9901 cgctgatgga ggcacgcgcc cctgccgtgc tcgacgcttt cgggcgattc gcacaggcac
     9961 tgccccggcc gcatgtcatc atgggcgagc agcaggtggt tgcacgcttc tggcgtggct
    10021 acgcggacgc gggcgcggcc ccgcgcctgt cctgccgtga gttgctcttc gagcagcggc
    10081 ggccggtcgg gggccgcgcc gcggtgaccg acctgcgcct cgccacgccc gacgacctga
    10141 acggtgtcat ggaagtgcag gcccgaatgg cgttcgagga gtgtggcgtc aacccgctgc
    10201 ggaccgaccc ggtcggcttc cgcgagcgct gccggcggcg gatcgagaag gggcgcgtgt
    10261 gggtggtgtc gaggtgcgga cggctggtct tcaaggccga cgccatcgcc gacacgccgg
    10321 agattatcta ccttgaaggt gtccacgtcc ggcccgacag ccggggcgag gggctggggc
    10381 tggactgtct ctcgcaactg gggcgcacgt tgctcgcgaa gtccgcctcc atctgcctcg
    10441 tggttaaaga ggggcgcacg gaggcgcagg atttttaccg caaggccggg tacgagttcc
    10501 gctgctatta cgacacgatc tatcccgcga agtgaggcgg ggcggagtgc gccgcggcgg
    10561 gctgcggcgg atgtcggagg gaaggggcga agcgatgggc gaagtgaatg cgaagaacag
    10621 gtacatgtgg gccatcatcg cggggggcgc gggcgtgttg gccgtctcgg cgtaccgcct
    10681 cagtccggcg ctgctcgacg tgaagttcgg cgtgctggcc gtcatcacca tcgtggtcag
    10741 ctcgcgcctc tccagcaaga ttccgcgact cagttcgcac atcaccgtct ccgacacctt
    10801 catcttcctc acgatgatgc tctacggcgg cgaggcggcc gtcctgctcg ccgccgtcga
    10861 ggggctttgc tcctcgctgc gcgtgaacaa gcggcccttc accactctct tcaattcggc
    10921 ggtgatggcg tgcgccacct tcctcacggt cacgaccctg cgcctgctct tcggcagcat
    10981 ccgcgagttg tcgcacgagg gctacacccc cctgttcgtc gtcgccgtct gcgtcatggc
    11041 gctggcgcag tacgtcttca actccggttt ggtctcggtg ctcgccgcct acaagatcga
    11101 ccggcccttc tggcacacgt ggcggaacta ttatctgtgg agttccatca cctatttcgc
    11161 cggggcgtcg gccgccggaa tcatcgccaa gctgatcacc gaggtcggct tctacgccgt
    11221 catcatcacc acgcccatcg tcgccatcat cttcttcacc tacgagacgt accggaataa
    11281 catcgaggcc tcggccgcgc agaccgcgca ggccgaacgc cacgtcgaag aactctcgct
    11341 ccacctcgcg gagcgcgagc aggccgagaa ggagcgcgac cgcctgctcg tgcgcgagca
    11401 ggcggcgcgc gccgaggccg agcgggccaa ccgcatgaag gacgagttcc tcgccacgct
    11461 ctcgcacgag ctgcgcacgc cgctcacctc catcgtcggc tggacggact tgctgcgctc
    11521 cgacaagctc gacccggcga ccagaatcat ggcgctgacg acgattgaac gcagcgctct
    11581 cactcaggtg cagctcatca acgagctgct cgacgtgtcg cgcatcatca cgggaaagct
    11641 cctgctcgac attcaggagg tcgatctcgc gacggtcgcc gcggccgcgg tcgaagtcat
    11701 gcgcccggcg gccgtcgcca aaggcatcac cctcgtgcag agctgcgcgc cgggcgccga
    11761 actggtcagc ggtgacgcgg cccgcctcca gcaggtggtc tggaacctcg tggcgaacgc
    11821 cgtcaagttc acgcccagcg gcgggcgcgt cgaggtgcgg gtcgagcggt cggtgacgca
    11881 cgtcgtgctc acggtgagcg acaccggggc cggcatcagc cccgagttcc tgccacacgt
    11941 cttcgagcgc ttccgccagg ccgacagcac caccacccgc acgcacggcg gcttgggcct
    12001 cggcctcgcc atcgtccgtc acctcgcgga gatgcacggc gggatggtcc acgccgagag
    12061 ccccggcgaa aacatggggg cgacttttta cgtgagtctc ccgccggcgg ccatcgaaac
    12121 agaggggcgg aacgcgcggc gcgtgcgccg gcaggacggc ctcgccgggg cggcggccgg
    12181 acggcccatc ctcgacgggg ttcgcgtcct cgtcatcgac gacgagcgcg acgcgcgtca
    12241 ggtgatcagt gccgtcctca cgcattcggg ggccgaagtg catgtctgcg actctgccag
    12301 cgacgccctg aaggagttgg aagggtggac gcctgacgtg atcatgagcg acatcagcat
    12361 gcccggcgag gacggctatg acctgctgcg gaaagtgcgg agccttcccg gcgagcgcgt
    12421 gcggagcatc ccggcggcag ccctgacggc ctatgcgcgc gacgaagatc gcgagcgcgc
    12481 cctggccgcc ggcttccaga aacacatcgc caagccagtg cgctcggccg agctggtggg
    12541 cctcgtcgcc gacctcgcgc gaatctgatt tgcaaaccag atgagctgtc cccgtggcgg
    12601 acactcacaa cacagagacc ggggggatgt tttgcctggc gggcaaaaca tccccccggt
    12661 ctctgtgttt agccgaactg tttccagctc ggcccaattc aaaactgact ctacccttga
    12721 gagttcatta atcgcggccc tctccttgat tgaagcgggt ggccctttcg ccgtccgtta
    12781 atcctcctta accttcgggt gacgaacggt tggcacgctc gcccgtgaag ctcaggcggg
    12841 acatccaacc ctccgccctg aattcatcac tcattaagcg catggacagt aacgacttca
    12901 ccaccaaggc tgaccagcaa ctggtcgaga agttcatgcg gactaaagac gaaacaacgt
    12961 tcagagaact ctatcgccgc cacaccccgt acctttacct gttggcgctg cgtctggtcg
    13021 gcggcgcggt aagcgaagcg caggacgccg ttcaggacac ttggctccgc gcctgcaaga
    13081 cgttgtccgg gttcgcgtgg cggtcgagtc tgcggacatg gctggcgggc attctcatca
    13141 accgcgtgcg cgagatgaac cgtgagagcg tgcgccgaaa cgaagaagaa ctgcctgagg
    13201 aattttcgct ccctgggcca gcgcgggcga gcgagcatgt ggacctcgag cagtccatcg
    13261 cccgcctgcc ggccggctac cggctcgtgc tcgtgcttca cgacgtggaa ggttacacgc
    13321 acgaagagat tggggcccgg ttggagatca gcgccggcac gtcgaagagc caactccacc
    13381 acgcgcgaaa ggctctgcgc gccgcgtttc aaggcaagtg aggttccgag atgagtcagg
    13441 aatttgaaga cgagctgagc gcggaagaac gtcgggcgct cgacgctctg gtgcgggaga
    13501 agacggcgct gccacttctg gaagagcgtg tcgtcggggc gctcaaacgg gcggagttgc
    13561 ttcgccccgc taagagtatc cggggccttc gcgcgccccg cgtcgggctg gccgtcgcgg
    13621 cgtccttgtt gttcttcgtg ctgggcacgc tggccggcgc gcgctggggc gctggggcgc
    13681 cggagagaaa gaccggcgtg ggggagttca tgctggtgct gcgctccgcc gcggggcagt
    13741 cgcagcccga cgcccccgac gaggtcaggc gcagagtcgg cgagtacggc aagtgggcgg
    13801 gacggctgag gcagcagggc gtgcgcgttg acggcgagaa gctcaggcgc gaggcgcgca
    13861 ttctgcgtgc tgccgacggg agcgctgcgg tctccgaaga acgttccgag gcgagccaag
    13921 acgccatcgc cggctacttc ctaatcgaag cccagaatta tgagcaggcc gtcaaggtcg
    13981 ccgaagggtg tccccacctg aagtatgggg ggacaatcga gattcggcag attgatcggt
    14041 tttgaaaact cgcgcccggc gcgaagccta aggaggaatg gaatgaaagt gctggctcat
    14101 ctcgcgtccg tcctgctgct cgtcggcacg ggcggcttcg ttaacaaccc tcccgccgcg
    14161 aaggcggagc ttaatgcggg tgtgagcgaa tctttcatcc gcacctttgg cgtgaagatc
    14221 aacgtcgaag acatggagaa agccctcgcc ttctacaacg gcaagctcgg ttttgaagtc
    14281 gaagaccggc gcggctatcc cgatgaagtg aagctggcat ccggcggccg ggaaaaaatc
    14341 atcctcaacc gcgtgaagaa gctcaggccg ctcgtcccca acgatgcgcg gctcaccttc
    14401 acgctgcaag tcaacgacct cgaccaggcc atccgcaggc tgaaagcgct cggggtagag
    14461 ttcgccgaaa cggaaaaacg taaagaggcc gtgggcgacg ccatctccat ccgcgacccg
    14521 ttcggcagac ccatttcgat gatgcaccag accgtcgtca aggtcgagcc gttcaaagag
    14581 ccgagggttt acaacttcgg cttcctcgtc cccgacatgg gcgtcggcag agatttttac
    14641 acgaacaagc tcggcttcgt cgtccgcagc gaaaaatact tgccgctcga tctgccgctc
    14701 ggacacccag acgcaacctt cgccttcatg ctccacgtcc gccccggcgt tcgccccgtg
    14761 aagagcgcct acccggaggc cgcgccgttt ctgacgatgg tcttcgagac cgccgatctg
    14821 ccgcagacgc tcgaagggct gaggcggaag caggtcaacg tggtcgccgt caaagattct
    14881 cagcagggcc gggtcgtcgt tctcgaagac cccttcggga acgtctcgga gatcgtcgag
    14941 gcgcgcaagt gaatccactg gcgtcggcga tttcgtctcg acgcgcatcg cttcgagtag
    15001 ggccgcgaag acaagggggt agttaccgac cggctcggcc gagtaacctt cgccggagct
    15061 aggaaagggc gcgccacttc gtgtggcgcg ccctttcggt tagagatcac tcagagcgat
    15121 aatttaattg actttttagg gtggggacga taatctccgc gccttcacaa cggcgggccg
    15181 gcgcccaaag cctcataaac ggcccgttaa accggttaca agcggttagt tcaattattc
    15241 tgtagtactc accgcgataa ccttttttgc cccgcaatgg ttgtcacact aggccagcgc
    15301 gagcctgacc gggaaccggc gaagtcttat cccgcggtca gcgtctcacc tgaccggcgg
    15361 gctgtgttag ctctttaacc cgacaggagc gccccgaaga ccgaccacca cacgagttct
    15421 ccctaaccct ccgtcgaatt aatttccaca cagagaggtt gctccgtact tgtaggggct
    15481 gggaaacctc aggcgcccac tgccgggagt cagaagaggc atagactatg agacccctcg
    15541 actcctcgac ggcgaggccg cgccccggcg gccgccggcc acgcgtcgcc ggcaagttcc
    15601 tctacgcggg cgaccagaag ctctacgtcc gcggcgtcac ctacggcacg ttccgccccg
    15661 gccccgacgg gcgcgagcgg ttcgaccccc agcaggtcgc gcgcgacttc gcgcagatgc
    15721 gcgaggcggg cgccaacgcc ctgcgcacct acactgtccc cccgcgctgg ctcctcgacg
    15781 cggcccacgc ccacggcctt tacgtcatgg tcggcctgcc ctgggagcag cacgtcgcct
    15841 tcctcgacct gccgggccgc gcgcgcgaca tcgagcggcg ggtgcgcgag atggtgcgcg
    15901 agtgcgcgga ccatccggcc gtactctgct acacaatcgg caacgagatt ccgtcgccgg
    15961 tcgtgcgctg gctcggcgcg gcgcgagtcg agcggttcct gcgccgcctc tacaaggcgg
    16021 tcaagaagga ggacgcgggc gcgctcgtca cctacgtcaa ctacccctcg accgaatacc
    16081 tcgacctctc cttcgcagac ttcttctgct tcaacgtcta cctcgaatcc gaggagcgtc
    16141 tggacgccta cctcgcgcga ctccacaacc tcgcgggcga ccgccccctc gtgatggccg
    16201 agatcggcct cgacagccgc cgcaacggcg agggcgcgca ggccgagacc ctcgcttggc
    16261 aggtgcgcac ctgcttcgcc gcggggtgcg ccggcgcgtt cgtcttcgcg tggacggacg
    16321 agtggtggcg cggcggccac gacatagagg actgggactt cgggctcgtc acgcgcgacc
    16381 ggaggccgaa gcccgcgctc gcggcggtcg cgcgagcctt cgacgaagtg ccgttcgcgc
    16441 ccggcggcgc gtggccgcgc gtctcggtcg tcgtctgtag ctacaacggc gagcgcacca
    16501 tccgcgactg cctcgaaggg ctggcggaga tcgactaccc gaacttcgag accatcgtcg
    16561 tcaacgacgg ctcgaccgac cgcacgccgc gcatcgccgg cgagtacgac gtgcgtctca
    16621 tcagcacgca caaccgcggc ctgtcggccg cgcgcaatac cggcgcggag gccgctaacg
    16681 gcgagattat cgcctacacg gacgacgacg cgcggcccga ccctcactgg ctctactacc
    16741 tcgcgcactc gttccggcgt tacggcttcg ccgccgtcgg cgggccgaac ctcgcgccgg
    16801 ccggcgacgg actcgtcgcc gactgcgtcg ccaacgcgcc gggcggcccc gtccacgtgc
    16861 tcctctccga ccgcgtggcc gagcacatcc cgggctgcaa catggccttc cgccgcgagg
    16921 tcgtgcagga ggtcggcggc ttcgacccgg cgtaccgcgt cgcgggcgac gacgttgacc
    16981 tgtgctggcg cgtgcaacgg gcgggcggca cgctcggctt ccacccggcc gcgttggtct
    17041 ggcaccaccg gcgcaactcg gttcggactt actggcggca gcagtggggc tacgggaagg
    17101 ccgaggcgct gctggaggag aagtggccgg agaagtacaa cgcggccggg cacctgacgt
    17161 ggggcgggcg actctatggc aaggggctga cacgcgccct gctcttccgg cgcgggcgcg
    17221 tctatcaggg gacgtggggg gcgggactgt tccagtcggt ctacgagccc gccgcgggca
    17281 cgctccgctc gctgccgctc atgcccgagt ggttcctcgt cgtcttcacg ctcgcggcgc
    17341 tcgcgtcgtt cggcctcgtg tggacgccga tgctcgcggc cgcgcctctc tttgcgctcg
    17401 cgctcgccgc gccaatcgcg caggccgtac tgagcgcgcg ccgcgcgcag ttcacgggcg
    17461 ccccggagac gcgcggcgcg cttctcaaac ttcaacttct gaccgccacg ctgcacctga
    17521 tgcagccggt ggcgcgtctg ttgggccgcc tgcgctgggg cctgacgccg tggcggcgga
    17581 ggggcgtcgg cggcttcgcc gcgccgcgcg cgcgtctgac gagcgtgtgg agcgagacgt
    17641 ggcgcgggag cgaggagcgt ctgcgcgacg ttgaagcgac cttgcgcgcg ggcggcgcga
    17701 cggtgctgcg cggcggcgac tttgacgcct gggatttgga ggtgcgcggc ggactcttcg
    17761 gcggcgcgcg cgctctcacg gccgtcgagg agcacggcgc ggggcggcag ctcgtgcgcg
    17821 cacgctcttg gccccgcgtc tcgccgctgt ggctcgcaat cactttactc ttcgcggcgc
    17881 tcgcgtgcgc cgcggcgtgg gagggcgcgc agacggccgc gctcgccttc gcactctccg
    17941 ccctcttcgt cgcggcgcgc atcgtccttg agtccggcgc ggcgatggcg acgatgctcg
    18001 atgcgttcgt gaggccggcc gcggcggagc gcgagaggca cgcgcgagcg cgcgaggtgt
    18061 tgaaggtgac gcctgcggcg ccggggacgc tggccgcggc cccggccgca ttgcgagcgg
    18121 aggcggctgt cgaggtcggg cgtgtccctc cgtccgtgtc cgaagtggtc gcgcagttcg
    18181 aggcggaagg ttgacgggtt cacacggcct tgagcatcca tgagctttag cggcgaagga
    18241 caagacgcca cgcactcaac ttgttttcac gattcacgat tcacgattca cgaacccatg
    18301 agcgagcgtc tgacataccg gctgctttgc gaagacgacc tgccgggcct gctgcggctg
    18361 tgggaggggg cggggtgggg cgtgctcacg cccgagcagt ggcgcgcgtg gttcgtcgag
    18421 ggtccgcagg ggccttcgct cgtgacggtg ggcgtggacg agcgcggcga ggtggcggcg
    18481 caggagatgt tcgcgccggc gcgcgcggtg gtaggcgggc gcgaggtgcg cgcgctcagg
    18541 ttatcggccc ccatcctgcg cgaggaattg cgcgggcgct cgctcaagaa cgcggaacac
    18601 ccgatcatcg gcctcttaaa ggtgtcgatg tcggcggcgg ccgagcaagg attcgcggtt
    18661 gtctattcgc tgccggaata cgcgtggctg cccgtcttcc gacgcctccc gagctacggc
    18721 atcaagccct tcgcagaggc cgtctacgcg tgcgcgtcgc tgcccttcga ggccgcgcgc
    18781 tccacccctg tgagaagcgc cgcggcgggt cttgaggcgc gcgccgtcga ggagttcggc
    18841 gaggaattcg aggagctttg gcgggaggcg aagtcgagtt ttccgattga ctgcggcgtg
    18901 gtgcgcgaca agtcatggct cgctttccgc aattcgggtc gcatcgcggt cgaggtgcgc
    18961 gccgcgtctg acggcaggct cgtcggctac tcggcgacga agcgggcgac gggcctgctc
    19021 gccgacctgc tcgcgcgcga gcccgcggag ttgaagctgg tagtcgccgc cacgctctgc
    19081 tggctcgaag cggagcgcgt ccgatccgtg cctgaaggtt tgacgcacct gaaggtcatg
    19141 cggacgccgg cgctcgcgcc cgcgcttgac gcgctcgggt tcgagccgtc cgactacagg
    19201 ttcgccttca cgtgcaactc gttcgatccg tcgctcacgc tcgaagagat tgcgcctgag
    19261 cgttggtacg tcctgcccgg tgattgagga ggttcgcttg gttctcgctt acaggctcgt
    19321 gcgggaggac gacttccctg aagtgcagcg cctctggaag gaggaggccg gctgggggac
    19381 tttcgccgag gagatgtggc ggcgctacgt cgtcgaggcg ccgttcggcg gggtttcggg
    19441 gacggtggcg acggacacgg acacgggccg catcgtcggt cagttcgcct tcgtgccgtc
    19501 gctcgtctcg gtggacgggc gcgaggtgcg ggccttccgg cccggcgccc ccgtgatcgc
    19561 gcgcgggctt cgcttccgca gcgcgaaccc gttcagccac cccatcgtgg cgatgtataa
    19621 gttcgcggtc aaggcgttgc gcgagcgcgg cgatggcctc atctacatgg tgccggacag
    19681 gcgttgggcg cgactcttca agatgttccc gttccttcag accggctcgt tcccgctctg
    19741 gcgactcgct ctcccgacgc ccgcgccgct cccgctcggc gagggcttca cgtcggcgcc
    19801 gctccgcgag tgggacgcgc gggttgatcg cctgtgggcg gattggcgaa aatttcacgg
    19861 ctgcatggtc gtgcgcgact cgcgcacgat gccgtggaag ctgggcgact cgcggcacga
    19921 actggtcgcc gtcgagcgcg gcggcgagct ggtgggcttg tcggcttcgc gccgccgcaa
    19981 gggcgaggag cagtggctcg tctgcgacct actcgcggcc gacacgggcg aagccttgcg
    20041 cgccaccctc gccgccaccg ctaacctcgc gcacgcgaag gcgctcgaat ccgccgcgac
    20101 ggagaagccg atacgcaaga tgaccgtgct cgtcacgccc ccgatggagg cggccgtgcg
    20161 cggcctcgga ttcgcgcgcg acgattacga tttcccgctc gtcgtccacg tcctcgaccg
    20221 gtcgctcaag agtgatgttc acccctcgcg ctggttcgtc agcgcgaacg actgaaacga
    20281 ctatggccct caaactcgaa ttttatgagg tggccgatct gcccggcctt gagcagctct
    20341 ggcgcgagga gacggactgg ggcgacgagg tgctgcggcg gctccacgag tggttcatgg
    20401 cggcgccgtt cggcccgccc cgcattgtcg tcgcgcatga ggcgggatcg aacgaaatcg
    20461 tcgggcagtt ccgcttcatg ccgacgcgcg tctcggtgga tggccgggag gtgcgcgcca
    20521 cgcgcccctt cggcaccatc atcaccgacc gtatgcgcgc ggcggtcgag agcacgaacc
    20581 ctcttgagca gcccgcggcg gcgatgtacg tccgcgccgt cgaggagctg cgggcgcgcg
    20641 gcgaccagct catctatatg gtccccgacc cgcgctgggt gcgtatgttc aggatgttcc
    20701 ccttccttca aagcggctcg ttcccactgt ggagcctgcc cgtgccgctc gcgggcgggc
    20761 cgctcccgct cggccaaggg tacacgtcgg ggccgctcgc ggcttgggat gacgggcgca
    20821 cagaccgcct gtggaacgcc gcgcggcgac agtacggctg catgctcgtg cgcgacgcgc
    20881 agacgctgcg ctggaagctc gcgaacgcga accacacggt gacggccgtc gagcgcggcg
    20941 gcgagctggc cgccctcgtc gcctcgcgcg cgaagggcga ccgccagtgg ctcctctgcg
    21001 acctgcttgc cgcggacgac ggcgaagcac tgcgcgccgc gctcgccgcg gccgccaacg
    21061 tcgcacatgc cgaggcgctc gcacgcacgg gcgcgcagga gattcgtaaa gtggccgtgc
    21121 tcgcaacgcc ctcgatggag cctgtcctgc gcgacctcgg attcgcgcgc gatgattacg
    21181 acttcccgct cgtcgtccat gtcctcgacg acaccctgcg ggccgaagac ttcaaccctt
    21241 cacgctggta cgtgagcgcg aacgactgag cgcgcgggcg cggaaatttt tcggaggcgg
    21301 agacggcgat gccgaagtac agcacagaga tttcacacgg gctcgggcag gcggaggcgt
    21361 tggcgcgtct gaagtccatg accgagcagg ccggacgcgt ctccgacctg aagggcactt
    21421 ggagcgaaaa cactttcgag ttctccgtca cgacgcaggg catcggcctc cgcggcacgc
    21481 tccgcgtcga ggccgacgcg ctcaagttcg acggccgcct gccgctcatc gccatgccgt
    21541 tcaggaattg gattccgcgc gtgctaaaga aggggctcgc gcaggcgggg acaagcgttc
    21601 gggaggccgc cgccccttcc cccgccgaag cggacgcgcg ggtcggcgcc gagccggacg
    21661 cacccgccgt cctcttcctg cacatcccga aggccggcgg gcagacgctc ggcgagtacg
    21721 tctacaacca ctgccgggac gccgaggcgc gcgccgcttc cgttgccgac acgctcgacg
    21781 cgggcgtcgc ctacctcaac tacggctttc tcaaggagcc gggcctcgtc gtccccgagc
    21841 acgtccgacg gctgctcggg cggcgcgacc tgcgggccgt catcggccac ttctgtttcg
    21901 gcctccacga gcacgtcgcg cgtccgcacg tttacgtcac gctcttgcgc gaccccgtcg
    21961 agcgcgtcgt ctcgctctac cattacgcga agctgcacga gacgatgggc ctcgaagagt
    22021 tcgtcggctc gccgcccttc aaagaggtgg acaacgacca gacgcgccgc ctcgcgggcg
    22081 ttgacccgcc gctcggccgt ctcacgcgcg acgccctcga cgcggcgaaa gagaatctgc
    22141 ggagccactt cgccgtcgcc ggcacaatcg agcggttcga cgagaccctc gcgctcctcc
    22201 gtctgaaact cggctggacg cgcgaggtcg tctcctaccc gcgcaacgtc aatagcaagc
    22261 gcccggccgt agcctccttc ccgcgcgaga caatcgaggc ggtgcgcgcg cgcaacgagc
    22321 ttgaccttga gctgtggcaa tacgcgtcgc gactgatgga cgaggcgata gccgacacgg
    22381 gcgagaagtt cgccgacgag ttggaaagat acggagcgtt gaagggcgca tgagcgagaa
    22441 actgcgcatc gtcgtcggcg gttacgtggg cctgctcccg gcggggggag tgacgtggga
    22501 ctacgtgcaa taccccgcgg ggttcgccgc gctcggccac gacgtttact acgtcgagga
    22561 cacgcggctg tggccgattt atcagcccga ggggagcgac tggggcgacg cctccgcctc
    22621 ggtcgcgcac ctgcggcgcg tgatggacgc cttcgacctc ccgtcacgct gggcctaccg
    22681 cgacgaggca tcgggccgca tcttcggcat gagtgaggaa aaacttcgag aggtctgccg
    22741 cacggcggat gtgttcgtca acgtctcctg ctcgacctac atgcgcgaag agtaccggcg
    22801 catccccgcg cgcgcgctca tagattccga cccgatgttc acgcagattc aatacctgtc
    22861 gcagcagatg ttcacgccgg gcgagcccac ccagcgcgac ctcgttgacg cgcacaactt
    22921 ccatttcacc ttcggcgaga acatcggcca agaagattgc tccgtgcccg actgcggtat
    22981 cgagtggcgg cccacacgtc agcccgtctg cctccaacac tggcgcgcga cgcccccgca
    23041 ctcgggcgca gtcaacgatg agggcgcggc ctacacaacc ctgatgaact ggacggccgc
    23101 gcgcccgctt caacacgacg gcgagacgtg ggggcagaag gacgtggagt tccgccgctt
    23161 ccttcatctt ccttcgctcg cgcccgaagt gacgctcgcc gtcgcggtcg ggcagacggg
    23221 cgggacgggc gagcctttcc ccgccgccga ggcgcgcgcc gctggctggc gcgtgctcga
    23281 ccccgaagtg tgcgcgcccg agtggcgctc ttaccgcgac ttcatcgagg cttcgcgcgg
    23341 cgagttctcc gtcgcaaagg agacttacgt gaaggcgcgg acgggctggt tctcgtgccg
    23401 ctcggcctgc tacctcgcgt cggggcgcgc cgtcgtcacg caggacacgg gatggtcgaa
    23461 gttcctgtca tcaagtgaag ggctgctggc cttcgacacg ccggaggggg cgggcgacgc
    23521 gctgcgccgc gtcgaggccg accccgcccg ccattccgcg gcggcgcgcg ccgtcgccga
    23581 agagcacttc gactcgtcgc gcgtcctttc gtcgctacta gcggagatgg gcggctgacc
    23641 gtacgaacaa ggaaggagtt cgactgacgt gccggaagca atcgaacaga gagtgatgaa
    23701 ggccatcgcc gcgacggccc acgcgccaat cgagcaggtc acgcccgaga cgactttcga
    23761 ggagttgggg atgacttcgc tcgacgcgct cgcgctcatc gccgacctcg aagaagagtt
    23821 ccgtatagag attccgaacg aagacgcgct gtccttgcgc gacgtgcggc aggccgtcgc
    23881 gtgcgtgcgc gcgctgctgc cggaagcttc aggcccggaa gcgaggccgt cgtgagcgcg
    23941 ccgcgccggg tcgtcataac gggcatgggc gccgtcacgg cgctcggccg caacgtcgct
    24001 gagttctggg acgcgctcgc ggcggggcgt tcaggcatcc gcccgctcga aaccgttgac
    24061 cgctcacgcc tgcgcttcga gaacggcgcc gaggtgcgcg gctacgaccc ggccgggcac
    24121 ttctcggaca aggaactcga cctgctcgac cgcttcgcgc agttcggcct cgtcgccgcg
    24181 cgcgaggccg tcaaagacgc gggcgtcgag tggacgcgag acttgcgcga gcgcacatgc
    24241 gtcgtcacgg gctcgtgcct tggcgggaag atttcggagg acgaggcgtt cggcggcttc
    24301 tacgccgagg gccggcgcgc gcacccgctg gcgattccgc gcgcgatgtc gaatgccgcg
    24361 gccagccaca tctcgatgga gttcggaatc ctcgggccga cctacaacgt ctcgacggcc
    24421 tgcgcctcgg cgacgcacgc catcgggcag gcgttcgcgc tcgtccgttc gggcgcggcc
    24481 gagttggccc taacgggcgg gtgcgaagcg cccttcgcgc tcggccatct gaagaagtgg
    24541 gaggcgctca gggtcgttgc gcccgacaca tgccgcccgt tctcgaagga caggcgaggc
    24601 atgattctcg gtgaaggcgg cgcgatgctc gtgctcgaac cgctggacgc ggcgctcgcg
    24661 cgcggcgccc gcgtccacgc cgaagtcgtc ggcttcggca tgtcagccga cgcgcaccac
    24721 gtcacgcagc ccttggccga gggcgcggcg cgcgccgtgg cggcggcgtt gcgcgacgcg
    24781 gacgtgaggc ccgaacaggt cggatacgtc aacgcgcacg gcacggcgac ggccgcgaac
    24841 gaccccgccg agaccgaggc gctgcggctc gtcttccgcg agcacgccgc gcgcctcgcc
    24901 gtcagctcca cgaagtccat gcacgggcac gccctcggcg cggcgggcgc gattgaagcc
    24961 gtggcgacgg ccctcgcgct gcgagaaaaa cttctgccgc cgacggcgaa ctactcggag
    25021 ccagacccgg cctgcgacct cgactacatc ccgaacgagg cgcgcgcggc cgaagtcggg
    25081 tacgccgtct cgaactcttt cgccttcggc ggactcaacg gagtcctcgt cttgaagagg
    25141 tgggattcgt gatgaccgaa gcgcggctct tccatccgct cacgccgggc gcggagcttc
    25201 tgggcgactg gttcgcgggg cgcattccct tgaacgtcgt cgcgggcgaa gggacggtca
    25261 tcgactcttc gttctgcttc aagcacttct actcggcgcg cgaagtgggc ttgaaggtcg
    25321 gcggccgcac gaccatctgg cgcgcctcgc tcgcggccga cgaggaggcc gtcatcgaga
    25381 tcggcgacga gtgttacatc gccaacgcct cgctcgtctg ttccgaactt atcagcatcg
    25441 gctcccgcgt catgatcgcg ggcggcgtta ccatcgccga ctccgacttc cacccgctcg
    25501 gccccgcggc gcgtctcgcc gacaccgtcg cgctctcgcc cgtcggcgac cgcagacgga
    25561 ggcccgccgt cgagacgcgg cccgtccgca tcgaggacga cgtttggatc ggctacaacg
    25621 cggccgtgct gaagggcgtt cgcgtcggcg cgggcgcgct cgtcgccccc ggcgcggtcg
    25681 tcatacgcga cgtgccgccg ggcgccgagg tcgcgggcaa tcctgcgcgg gtggtgtgtg
    25741 agtagatgaa agagaacatt ccgacaagcc tggcccctga tgtcggcgcg cgccgtcatc
    25801 tcgaacacga ctggtacgcg ggcggcatcc ccgccaacgt ccgcttcgcc gagggtgtct
    25861 acgtagatac ctcttatggc ttcgccgcgt ttcactcgga gtgcgccgtg ggactcgcgc
    25921 tcggcgacgc ggcgggcgct tatgatcgca ccacctttgt cgtcggcccg gcgggccaga
    25981 ttaacgtcgg cgcgtacacc gtgcttaacg gaacttacat cgtctgcaac ggacggattg
    26041 acatcggcgc ccactgcctc gtcgcgtggg gttctgtcct gacggacacc tgggcggacg
    26101 cggcggccct gcccgtcgcg ttgcgacgcg aggtgctgcg cgcgtgcgcg gccgacccgg
    26161 cgcggaggct ccccgccttc gccgagccgc gcgcggtcgt gctcgaagat aacgtgtggg
    26221 tgggttttga cgcggtggtg ctgccgggcg tgcgcgtggg gcgcggctcg gtcgtgggct
    26281 gcaagacggt cgtcgcggag gacgtgccgc cctatgcggt cgtcgtcggc tcgcccgcgc
    26341 gcgtcgtccg ccgcctcgac gccgacgaca cggaggagga gcgaaagcgc gcggtgcgcg
    26401 agtacgcgcg ctgactttaa aagatggaaa ggcgcatctc catagacttg atcgtctgca
    26461 cgtacaacaa cgccgccctg ctcgacagga cgttggacgc gctcgcgcgc cagcgcgtca
    26521 ggcccgaagt tgattggggc gtgctcgtcg tcaacaacaa ttgcacggac gagacgccgg
    26581 aggttttgga gaagcacctg cgcgcgaccc cgttgccgct gcgcgtcgtc gtcgaatctc
    26641 gacagggatt gacgcccgcg cgcgtccgcg gcgtgcgcga gacggcgagc gagtgggtcg
    26701 cgttcgtgga cgacgactgt ctgctcgctg aggattgggt cgagcaggcc gcgcgcttcg
    26761 ccgtcgagca cccggagtgc ggcggcttcg gcgggcaggt gatccctgag tgggagacgc
    26821 cgccgccgcc ctacgtactc ggcaacctct acgcctacgc gtgcaagagc cacggcgaga
    26881 ggccccaccg gcgcccgtgg ctcgccgggg ccggcatggt tgtcaggcgc gaggcgctga
    26941 agagttgcgg ctggctcgaa cggcaatttc tcgaagaccg caagggcagc cggctcgtct
    27001 cgggcggcga catggagatc gccctgcgcg tcgccgcgcg gtacgaactc tggcacaacc
    27061 ccgcctgccg cctgcgccac ttcatccccg cgcgccgcat gtcgcgcgag tacctgcgcc
    27121 gcgtgacgcg cggcctcggc gcgagccgtc acaacgccga ggcgctgaag tggaagggat
    27181 catacgcggg atggctcctg tattcgtccg gcctctcgtt gggcttcggc gcgcttggcc
    27241 tgctccggct cgcgcgcgga ctcttcagtt cagaagttga tttcacgacg gccttcagcc
    27301 ccgccgccgg ctggtgttcg gcgatgtggg ggatgctgcg gatggacgcg gccgagcggg
    27361 gcgagcttat cggctgcgcc gccgcgcgct tcgacaggcg aggatgagag cgagccccat
    27421 gcacgcgagc gaaaccgagt tcgtgacgac cacgacgacg cgcgccgcca cggcgcgcga
    27481 aggcgcggcg cccgcgctga ccgtcgcact cgttgactgg agccaactcc tcgaagacta
    27541 cctcgacaac atcggcgtct ctttcgagtc gttccgcgat gagatgaccg gcgggtggat
    27601 gttcggttac gtcgaggcgt tgcgtctggc cggggtgcgc gcggttctgt tctgcgtctc
    27661 ggcgcgcgtc acagaaacag aacgcttcac gcacgcgccg acgggcgccg ccctccgcgt
    27721 gctccccgcg ccccgcgcct atcggcgctt gcggcggcgc gtgctcaacc cttacgcaac
    27781 gacagtcgaa gaggcggtcg gggtggttga agggcgcgcg cgccgcgcgt gctggtcggc
    27841 ggcgagggag ttgatgccgt atctttcgac cccggtcgtc gcgctcgctc gcgagttgcg
    27901 ccgcgagcgt tgcgacgcgc tgctcgtgca ggactacgag cacgggcgct tcgacatttg
    27961 cctcgcgctg gggcgactga cgggccggcg cgtctacgcc acctttcagg gcggcgactt
    28021 ttcgcacgga cgacttgaac gagtgacgcg ccgacgctct ttgcggcagg ccgacggctt
    28081 cgtcgtcgcg gggcgcgggg aggctgagcg cctgcgcgag cgttacggtc tgacggacga
    28141 gaagatcgct cgcgtcttca acccgctcga cctgtcggag tggcggccgg gcgaccgcgc
    28201 ggcggcgcga gctgcgctcg gcatcccgga gtcggcgcgc gttgtcgtct ggcacggccg
    28261 cgtagacttc cggcgcaaag gtctcgacgt gctgctcgaa gcgtggaggc gggtctgctt
    28321 tgagcggcct gacggagact tgcgtttgtt cctcgtcggc gcgggcaacg acacggaaga
    28381 gttgcgcgcc gccgtctcga aggcgtccgc cgagggcgtg cgcggtctgg ttcgggttga
    28441 cgaatacgtt acaggccgcg ggcggctgcg cgcgtacctg cgcgcggcgg acgtttacgc
    28501 cttcccctcg cgccacgaag gcttcgccgt cgcgccgctc gaagcgatgg cctgcggcct
    28561 gcccgtcgtc gcggccgacg cgcccggcgt gcccgacctc ctcgaaggcg gggaagactc
    28621 cggcggcatt ctagtcccgc gcggcgacgc gtccgccttc gccgccgcca tcgttcgcct
    28681 tctggacgac gaggcgctgc ggctcgcgct cggcgcgcgg gcgcgggcgc gcgtcgccgg
    28741 ggagttcgcg cttgaggcgg tgggactacg cttgcgcgat tttatgttga aagggggatg
    28801 ccaagattga cgcggctcgg ggcgaacggc aaagttttcg gtatcggact gagcaagacc
    28861 ggcacgtcga gtttgacgga cgcgctcaat ctgctcggca tccccgccgt gcattacccg
    28921 cacgacgagc gcactctttg cgagctgcgc gcgggccgtt accggctctc gctcctcaaa
    28981 gagtttcaag gcgtcacgga catccccatc gccccctttt acgcgcagct cgaccgcgcc
    29041 tttcccggca gccgcttcat ccttaccgtg cgcgagcgcg aggcgtggct gcgctcgtgc
    29101 gaagtccact ggcggctgat ggacgagtgg ctcgacaact tcccctactt caaacgcttc
    29161 cacgagttcg cctgcttggc cgcctacggg acactccagt tcggccgcga gcgattcgcc
    29221 cgcgcctacg acgagcacga gcgcggagtg cgtgactatt tccgcgaacg ccccggcgac
    29281 ctgctcgtcc tagacatctg cgggggcgac cgttgggaaa agctctgcgc gttactcggc
    29341 ctgcccgcgc ccgccgtacc gttcccgcac gcgaacgagt ggatgcacct gctgcttgag
    29401 gcgtcggcgg atgtggcccg caccatcccc gagggcgaga ccttcatcct cgttgacgag
    29461 gagggct
//
LOCUS       DASVES010000493        16738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_685876, whole genome shotgun sequence.
ACCESSION   DASVES010000493 DASVES010000000
VERSION     DASVES010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685876"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VF240_20780"
     CDS             <1..1229
                     /locus_tag="VF240_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177707.1"
                     /translation="LMKRWKKIALVVVGLLILSQAPFVYRRYRLGRLRAAIDSVNASR
                     APAAEGDPFVDYKGVLHVHSSLGGHSTGTLDEIVQAARMNELAFVVMTEHPSKYVDTA
                     AATLKGTHEGVLYINGSELSATNDERLFVVPGPAVAGPSAPDAQALVERARAEGGLAL
                     VAYPERIRDWGLLQGFDGIEVYNLYTNSKRIRYGLLFFDGLWSYRSHADLLFSTFYER
                     PDENLRRWDELTAARGLRLSAVAGNDAHSNVGLRLGDRTGKEFLGVSLDPYERSFRVV
                     RNHVLLDRAQPLAADSLVAALRAGRSYIAFDIFGDSTGFRFTAHSPSETKTMGDEIGL
                     PSGGEVRLKVRTPAAGRVVFFRDGQVIREFEEGTGAELAVGERGVYRVEIYLEGLRDF
                     LGERKPWIISNPIYVR"
     gene            1767..2390
                     /locus_tag="VF240_20785"
     CDS             1767..2390
                     /locus_tag="VF240_20785"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX7177708.1"
                     /translation="MASGARTAGPQSRLVEHVKRAVVVVNSYDERGRLLSQGSGFFVR
                     QTQITTNLHVVGRAARVEVVTFGGRVRVVEGVVAMDGRRDLALLQLDGAPEEVRPLVY
                     VESAPPAGEEVFVVSNPRGSLWEVSRGTTLDLREFPELGPLVPFTAAVARGSSGGPVV
                     DLRGRVVGVATMGLLRASGEDFFAVPAEHAARLRARTLMPFPLRNAD"
     gene            2744..3388
                     /locus_tag="VF240_20790"
     CDS             2744..3388
                     /locus_tag="VF240_20790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7177709.1"
                     /translation="MTETTTFPSIQVAARKIRGVSVTPPTSDFELAQAAAAGDMRSFE
                     ELYEKHHRRVYSLCLRMTQNVAEAEDLTQEAFIQLFKKIGSFRGESAFTTWLHRLTVN
                     QVLMHFRRRNVRMEQTTDDGDVPVQIVKGTENPSRMPVMDRIALDNAIGKLPPGYRSV
                     FVLHDVEGHEHEEISRLLGCSVGTSKSQLHKARMKLRTLLKKQKPPRMARRIKV"
     gene            3832..5985
                     /gene="ppk1"
                     /locus_tag="VF240_20795"
     CDS             3832..5985
                     /gene="ppk1"
                     /locus_tag="VF240_20795"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX7177710.1"
                     /translation="MTGKSRQTLPIDQHGAGASAPADSRSSAPDKILFNREVGLLEFH
                     RRVLEEALDAGQPLLERLKFLAIFSSNMDEFFMIRVSGLKEEIEHDVTEPSPDGMTPA
                     DQLHLIREHILPMFAEQTRCLLEEVLPGLKEHGVEVVPYKSLTDDERRGLDDYFEKHV
                     YPVLTPQSVDPAHPFPYVSSLSLNIGTMVRPLPEHGITSSLTGTGDPRFARVKVPPVV
                     PRLIPVGRTGSKFTFAEELIAANARKLFPRMHVEEFHAFRVTRDADIEVRQDEAGDLL
                     RVMQQTLRERKFGSAVRLEVAASMPRPMVEYLTRELGLTSEDVYVVEGPLGVQDFMQL
                     YKLDRPALKDKPLEATTPAPLRRGGGSFFEVIRRQDILLHHPYTSYDTVVDFIESAAK
                     DADVLAIKICLYRTGQKSPIPEALIEAAERGKQVTAVVELKARFDEEANIEWAQRLEH
                     AGVHVVYGVLGLKTHSKVALVVRREGEELRRYVHIATGNYNPFTSTVYTDLGLLTANE
                     EIGADATDLFNFLTGFSRQKTYRRLLVAPVNLREGMLALIEREAEHARQGRPARIAAK
                     INRLADLQIIRALYAASQAGVTIDLVVRGVCMLRPGVPGLSDTVRVRSIVGRFLEHSR
                     VFYFENGGEEEVYIGSSDWMSRNLDRRVEVVTPVLDPSLRRYLKDVVLAAYLRDNVKA
                     RVLQPDGNYVRLRPAAGEEGFSSQTHFEGAVSMNI"
     gene            6164..7195
                     /locus_tag="VF240_20800"
     CDS             6164..7195
                     /locus_tag="VF240_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177711.1"
                     /translation="MYTLGQTRRRSAFALALVLGLSTLTASAVQKDDKKKEPMPQGTA
                     VLWQDPGDISARNLLAGPGPDMKPDLSSVTFVKDEMGGYSPKFRVTDGAGKTWVAKFG
                     KEAQPETAAVRLAWAVGYVTEVAHLVPCVQIKNAPEPRKEVERCDGKGFANVRFEARP
                     DEWKRLDNWSWKENPFAGQKEFQGLIVMMGLINNWDLKDTNNKIVYVPGADSAAGELR
                     YIISDLGATFGKTGNFITHDRNNPKNFAKSKFIERVEGGRVRFSYDGKNTGLFDDISV
                     ENARWIGDQLARLSDKQIEDAFRAANYAGADLQTLVAEVRSRINELTALPAPAATAGT
                     PGSGTPDKR"
     gene            7202..8224
                     /locus_tag="VF240_20805"
     CDS             7202..8224
                     /locus_tag="VF240_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177712.1"
                     /translation="MRYPRQIHRLVSLALLTVLALSSLQPATAAPQKEAKGKQEDRKE
                     KKETPRGAPVLWRAPADIVTRDLYLGPGGESMRPDLRKVTFLKDETGGGYSTKFRVRD
                     ASGHEWVAKVGKEAQSETAAVRLIWAVGYVTEVVYLVPRLNVEGKGAFDNVRLEARPD
                     SVKRLDEWKWADNPFEGTKELQGLIVMMALLNNWDIKDTNNKVLYVRDEATGRGESRY
                     VVSDLGATFGRVKVNVPGVWRLQRSRNNPEDYWKDPFLEDVKNGQVFFFYKGKRQDLF
                     DDIRIDEARWIAALLSKLSDRQISDAFRAANYTPAEIRSLTEAVRARINELASIRGNA
                     PLASNP"
     gene            8420..9460
                     /locus_tag="VF240_20810"
     CDS             8420..9460
                     /locus_tag="VF240_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177713.1"
                     /translation="MSSATIIRRLCSSLLIIILAFTSAPLAAQDKSKNKKKKPTPRGT
                     PVLWRAPADIESRDLALGPGGAEMRPNLRRITFLKKQEGGWSTKFRVRDASGREWVAK
                     VGKEAQSETAAVRLVWAAGYLTEINYLAPCVRIEGAPAPGKDDEQCEGGGFANVRFEA
                     RPKGVKRLDPWEWKKNPFKGTRELQGLIVLMGLINNWDLKDTNNVVLAAPGARGRTEL
                     QYVISDLGATFGKTGSLPLFWRVTRSRNDPEGFSKDKFVDEVEDGKVSFHFTGKNRGL
                     FEDITVRDARWIGGWLSRLSERQIGDAFRAANYAPDEVQMLTDAVRRRVGELTALRGR
                     GAESGDAGGRRR"
     gene            9531..10478
                     /locus_tag="VF240_20815"
     CDS             9531..10478
                     /locus_tag="VF240_20815"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEX7177714.1"
                     /translation="MKRRDRSVFLLVGLAAAVLGFGIVAYMLSDRSAAPAGPADATTT
                     SAPTPAPTTQPTAAATTADARRAPTPTGAEGTPFEYLFSEDAQGAPARQETLSARVLR
                     IVWRLALAALLAAALAFRPRRDLAAHQRNLYVAQTQILLAVVASSLMMIVGDNAARAF
                     GIFAAVSLVRFRTNIRDPKEITVLLISLALGLATGVGRWELAIILTLFVFPLLWLLER
                     RESEEVFRTMELTVKTRNTEATQEMLREIFDRYEMSAEMRQIDPPDEAEPVGCIMYRV
                     SMNMNVSTDEINERILTIDPENVEGIEWEQKKNTGYVYN"
     gene            10595..11899
                     /locus_tag="VF240_20820"
     CDS             10595..11899
                     /locus_tag="VF240_20820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7177715.1"
                     /translation="MRTLTTLLLLASLVVTAAFAPAGLAQSRARRVGQSQPTATTPAP
                     QPRTPSAQTPGTADGARDRQVQEEVGDDEVVRVESTLVTIPVSVLDRDGRFIPALGKD
                     DFRIIEDGVEQQVAYFATVESPFTVALVLDTSGSTQFRLEDIQSAATAFVDQLRPDDR
                     VLVVSFDDEIRFLSEATSDRRQLRDAIRRTRPGGGTRLYDAFDAVIKQRLASVEGRKA
                     IVLFTDGVDTSSKRASYSGNMRDAEELDALVYPVQYDTYGSQQSGGGGWPGGGGGGGG
                     SIPRTGGVGDILIGILGGIGSRRGGGGPVTIGGGGGATREDYARAGRYLRELATLTGG
                     RHYQADDLSYLRQAFSNIAEELRRQYSLGYYPSKQAQASERRQIKVRVKRPNLVVRAR
                     DSYIYRPGGSTTTAQGDPNRQPSQTPPELRRKQFAKADAPIR"
     gene            complement(11986..13284)
                     /locus_tag="VF240_20825"
     CDS             complement(11986..13284)
                     /locus_tag="VF240_20825"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002772.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX7177716.1"
                     /translation="MPADKHDVLAVILGGGAGTRLFPLTKMRSKPAVPLGGKYRLVDI
                     PISNCINSDVLKIFVLTQYNSASLNRHVAQTYRFSHFSSGFVDILAAEQTPESPQWFQ
                     GTADAVRQVLPHIRDWGIDTLLILSGDHLYRMDYRDFLARHRETGADLTVSVIPFVRE
                     AAEEFGLLKADAEGRITEFREKPKGQALEEMRVDTSRFGLDPNEAERRPFLASMGIYI
                     FDFKKLEELLAANTAWVDFGKQIIPAAIERGNVQAFVFDDYWEDIGSISAFHAANLDM
                     TATIPKFNFFNSKAPVYTRARYLPPSKINNSHIQQSNITEGCIINRAHITRSIIGLRT
                     RIDHGAHIENSYLMGADWYQSLEEMRTEKAAGSPRVGIGEGAVIRNAIIDKNARIGAG
                     VRLVNEAGLREYDAPDGSVHIREGIIVVPKDATVPDGTTL"
     gene            13437..13880
                     /locus_tag="VF240_20830"
     CDS             13437..13880
                     /locus_tag="VF240_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177717.1"
                     /translation="MSLLAALGSGLAGACALTAIHETARRVLPDAPRMDVLGMRAISR
                     AFRAADAEEPPRAQLHDLALAGDILSNSLYYSLVALGGPKGAVACGATLGLAAGLGAV
                     ALPAPLGLGDGPSARTNETKAMTVAWYLAGGLAAGLAYSRLTRKR"
     gene            complement(13960..14856)
                     /gene="deoC"
                     /locus_tag="VF240_20835"
     CDS             complement(13960..14856)
                     /gene="deoC"
                     /locus_tag="VF240_20835"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX7177718.1"
                     /translation="MSHTNNGDLERLVEQITDVIVARLNGDGDGQAAMCGCSSECFDR
                     CPERMQRIVDAGASRIGLVLGQTGTARDWAGFVDHTLLKPEATDEDIRKLCAEAAQYR
                     FASVCVNPSWVRAAVCNLRGTSVPVCTVVGFPLGATLADVKAYEARRAIMDGAREVDM
                     VINVGALKSGDDCAVEHDIRSVVEASHEHGVLVKVIIEAALLNDDEKVRACLAAKRAG
                     ADFVKTSTGFAKGGATVADVALMRRTVGAGMGVKAAGGVKGIDDARAMLEAGATRIGA
                     SVGVKIAQEAAGVQTNASVAAY"
     gene            complement(14877..15083)
                     /locus_tag="VF240_20840"
     CDS             complement(14877..15083)
                     /locus_tag="VF240_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177719.1"
                     /translation="MRVESKDVKELFIVREQALDAAVHKAVRGALAVHKSAGNTVASW
                     EGGRVVLIPAERIAVRESFVKQRK"
     gene            complement(15080..15667)
                     /locus_tag="VF240_20845"
     CDS             complement(15080..15667)
                     /locus_tag="VF240_20845"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEX7177720.1"
                     /translation="MSEPRPQVVVIAGPNGAGKTTLAPFLLRDELGLSEFVNADDIAR
                     GLSAFNPQGATAQAGRIMLRRLRDLADARRSFAFETTLATRSYAAFLKRLKQKGYDFH
                     LFFMWLRSPDLAVERVRERVRLGGHDVPEHIVRRRYVRAARNFFHLYRSLADSWVVYD
                     NSVSGSPVRVAAGHGASALKVHDEGLWQRLSEVAK"
     gene            complement(15671..16522)
                     /gene="rpiB"
                     /locus_tag="VF240_20850"
     CDS             complement(15671..16522)
                     /gene="rpiB"
                     /locus_tag="VF240_20850"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX7177721.1"
                     /translation="MADDTQSTRERVRALVRDVLENALPAEDATTAGAASARPSESAP
                     SLSRAGADSGTTRARIIQTAPTGDGSEGPSFVRDESSKMVITEDDVRGLEEGARLRVA
                     EGARLTPLAADIVRERRIELVRRVSRRGSRALKIVAVGADHGGYPMKEELKKLLAELG
                     HQTRDFGTDSTDAVDYPDFAHAVARAVSEGEADLGIVVDGAGVGSAMTANKVPGVRAA
                     ACYSPKVARNSREHNDANVLTLGSGAITLDEMRDIVRVWLATEISEERHKKRVAKIEA
                     VERQYRS"
BASE COUNT         2918 a         5739 c         5276 g         2805 t
ORIGIN      
        1 gactgatgaa gcgttggaag aagatcgcgc tcgtggttgt gggcctgctg attctttcgc
       61 aggcgccctt cgtctaccgg cgctaccggt tgggccgcct ccgcgcggcc atcgactccg
      121 tcaacgcctc gcgcgcgccc gcggccgagg gcgacccctt cgtcgattac aaaggcgtcc
      181 tccacgtcca ctcctccctc ggcggccaca gcactggcac gctcgacgag atcgtgcagg
      241 cggcgcgcat gaacgaactc gcgttcgtcg tgatgaccga gcatccgtcg aagtacgtgg
      301 acacggccgc ggcgacgctc aagggaacac atgaaggcgt cctctacatt aacggcagcg
      361 agctgagcgc cacgaacgac gagcgcctgt tcgtcgtgcc cggccccgcg gtcgccggcc
      421 cctccgcgcc cgacgcgcag gcgctcgtcg agcgcgcccg cgccgaaggc ggcctcgccc
      481 tcgtcgccta ccccgagagg attcgcgact ggggactgct gcaaggcttc gacggcatcg
      541 aggtctacaa cctctacacc aactccaaac gcatccgtta cggcctgctc ttcttcgacg
      601 gtctctggtc ttatcgcagc cacgccgacc tgctgttctc gaccttctac gagaggccgg
      661 acgagaactt gcgccgttgg gacgagctga cggccgcgcg cggcctccgc ctgtcggccg
      721 tcgccggcaa cgacgcccac tcgaacgtcg gcctccgcct cggcgaccgc acgggcaagg
      781 agttcttagg cgtcagcctc gacccctacg agcgcagctt ccgcgtcgtc cgcaaccatg
      841 tgctcctcga cagggcgcag ccgctcgccg ccgactcact cgtcgccgcc ctgcgtgccg
      901 ggcgcagcta catcgccttc gacatcttcg gcgactcgac cggcttccgc ttcaccgccc
      961 acagcccctc ggagacgaag acgatgggcg acgagatcgg cctcccgtcg ggcggggagg
     1021 tgcgcctgaa ggtcaggacg ccggccgccg ggcgcgtagt ttttttcagg gacgggcagg
     1081 tgattcggga gttcgaggag gggacgggcg cggagttggc cgtcggcgag aggggcgtct
     1141 atcgcgtcga aatttatctc gaaggcttgc gcgacttcct cggggagcgg aagccctgga
     1201 tcatctccaa cccgatttac gtcagatgag cgcggcagag ccgggcgccg gatacaacat
     1261 cgccccgcac agagtaacgt ctaatgccgt acctgtgtgg ggcgctgtgc tgaatcgaag
     1321 cccgattcat tttttggagg cggtgcttaa gacaccggct atctgtagag aatcacgtgt
     1381 ttgattcttc gcacgcagac aatcccaagt gtgtagggag ggcgcgtgta cagcctccgt
     1441 aagttgtcca agtgagatgg attttatgct ccccgcgccc gtctgtcaat cctcagtttg
     1501 accgtctctg aaaaatacag cccgcgactc cgcaaggcag gccgttaaac acttttcgct
     1561 tccccgccag cgactcggcc gaacttcgcc gccactggaa tcctcccctg attaaacgtc
     1621 tgtctcagtt cacacggaac gattagacga ttctggcgcg ggcccacgtg ggtgcgcgcc
     1681 tcggggattt ttgtgtgcgc ctccgacggt taaaaaacag ggccgccatt ctaacgctcg
     1741 cgctcgtcgt ggcggtcgcc ccacccttgg cgagcggcgc acgcactgcg gggccgcaat
     1801 cccgtctcgt cgaacacgtc aagcgggcgg tcgtcgtcgt caactcctac gacgagaggg
     1861 ggcggctgct ctcgcagggc agcggcttct tcgtccgaca gacgcagatc accaccaacc
     1921 tgcacgtggt cggccgggcc gcgcgcgtcg aggtcgtcac cttcggcggg cgcgtccgcg
     1981 tcgtcgaggg cgtcgtcgcc atggacggca gacgcgacct cgcgctgctc cagctcgacg
     2041 gggcgccgga ggaggtgagg ccgctcgtgt acgtggagtc agcgccgccc gcgggcgagg
     2101 aggtctttgt cgtcagcaac ccgcgcggct ctctgtggga ggtgtcgagg gggacgacgc
     2161 tggatttgcg cgagttcccg gaactcgggc cgctcgtccc gttcacggcg gcggtagcgc
     2221 gcggcagcag cggcggcccc gtcgtcgatc tgcgcgggcg cgtcgtcggc gtcgccacga
     2281 tgggtctgct gcgagcgtcg ggcgaggact tcttcgccgt ccccgccgag cacgccgcgc
     2341 gtctgcgagc gcgcacactg atgcccttcc ccttgcgcaa cgccgactga agccgcccgc
     2401 tcgccgtccc gcgagcgatt tacccttcaa tctatccctg acgctccgaa gccagagcgc
     2461 cccgccgtaa tcctcttccc cgtggaggat ttgaatccca cgccggccgt cgctagccaa
     2521 ctttgtttgg cgctgcacgc tttcgtcgcc ggctgtctca tatggttgag cgaccactct
     2581 ggggggcgaa agtgacgggg aaactgcgcc attttagggt ttcggcccat ggcaccgggc
     2641 ttgcttctaa gcccgagacg gctaccagct tcgaagcttt atcggaggtc ggtcggccgc
     2701 gacggaaagt gttttcgtcg gccccacgcc ctttgaggtt agcatgacag agacaaccac
     2761 gttcccctcg attcaggtcg ccgcgcgcaa gattcggggc gtctcggtga ctcccccgac
     2821 ttccgacttc gagctggcgc aggccgccgc ggcgggcgac atgcgctcgt tcgaggagct
     2881 gtacgagaaa catcaccgcc gcgtctactc gctctgtttg cgcatgacgc agaacgtcgc
     2941 cgaggccgaa gacctcacgc aggaggcgtt cattcagctc ttcaaaaaga ttggcagctt
     3001 ccgcggcgag tcggcgttca cgacgtggct gcaccgcctg accgtcaatc aggtgttgat
     3061 gcatttccgc cggcgcaacg tccgcatgga gcagacgacc gacgacggcg acgtgcccgt
     3121 ccagatcgtc aaggggacgg aaaacccttc aaggatgccc gtgatggatc gcatcgcgct
     3181 cgacaacgcc atcgggaagt tgccgcccgg ctaccgctcg gtcttcgtcc tccacgacgt
     3241 tgagggtcac gagcacgagg aaatctcgcg gcttttaggc tgctcggtcg gcacctcgaa
     3301 gtcgcaactg cacaaggccc gtatgaagct gcggacgctg ctcaagaagc agaagccgcc
     3361 gcgtatggcg cgacgcatca aggtttaaga accgtttcgg ttccgaaggg gcggcgcgca
     3421 gtggccgccc cttcaaagtt tttacaacgc ttcccggttg acttcctccg cttcgactgc
     3481 tgcaagcatc atccttagta tgaaatcaaa acatacggcc gaataacgac taagggtcag
     3541 ccctcgaagc ggtaaagcct ggaaaaatgt cgagttttgc ggtaaaagta ccggacgctt
     3601 ccagggaata atgtttgctt taagatttgc tcgcgaagac gatgctctcc cgctaagagt
     3661 gctttctgaa gccaccaggg cggcgcgggg tcgcgccttt cggacttggg aaacaatcct
     3721 tgcgtgccaa ctcggcggcc tttgcataca tccggccgct cgcatggcgg catcaatcat
     3781 gttagaaata tcccgagata gctaatttca acagcgcgct ccagactaac gatgacagga
     3841 aagagccggc agaccctccc aatcgaccag cacggcgcag gtgcgtccgc tccggcggac
     3901 tctcgctcgt ccgcgcccga caagattctg ttcaaccgcg aggtcggact gttggagttt
     3961 caccggcgcg tgctcgaaga agccctcgac gcggggcagc cgcttctcga acgtctcaag
     4021 ttcctcgcca tcttctcgtc gaacatggac gagttcttta tgatccgcgt gtcgggactg
     4081 aaggaggaga tcgagcacga cgtgaccgag ccttcgcccg acggcatgac gccggccgat
     4141 cagttgcacc tcatccgcga gcacatcctg ccgatgttcg ccgagcagac gcgctgcctg
     4201 ctcgaagaag ttttgcccgg cctcaaggag catggcgtcg aagtcgtccc ctacaaatct
     4261 ctcacggacg acgaacgccg ggggcttgac gactatttcg agaaacacgt ctatcccgtg
     4321 ctcacgccgc agtcggtaga cccggcgcac ccttttccct acgtctccag tctcagcctg
     4381 aacatcggca cgatggtgcg gccgctgccc gagcacggca tcaccagctc tctgacgggg
     4441 acgggcgatc cgcgtttcgc ccgcgtcaag gtgccgcccg tcgtcccgcg cctcatcccg
     4501 gtcgggcgca cggggtcgaa gtttacattc gccgaggagt tgatcgcggc caacgcgcgg
     4561 aagctcttcc cgcgcatgca cgtcgaagag tttcacgcct tccgcgtgac gcgcgacgcc
     4621 gacatcgagg tgcggcagga cgaggccggc gatctgctgc gcgtgatgca gcagacgctg
     4681 cgtgagcgca agttcgggtc ggccgtgcgg ctggaagtcg ccgcgtcgat gccgcgcccg
     4741 atggtcgagt acctgacgcg tgagctgggc ctcacgtccg aggacgtgta cgtcgtcgag
     4801 gggccgctgg gcgtgcagga cttcatgcag ctctacaagc tcgaccgtcc cgcgttgaag
     4861 gacaagccgc tggaggcgac cactcccgcg cccctgcgcc gcggcggcgg gtcgttcttc
     4921 gaggtcatcc gccgtcagga catcctcctg caccacccct acacgtccta cgacacggtc
     4981 gtcgatttca tcgagagcgc ggcgaaggat gcggacgttt tagccatcaa gatttgcctc
     5041 taccgcacgg gccagaagtc gcccatcccc gaggcgctca tcgaggcggc cgagcggggc
     5101 aagcaggtga cggccgtcgt cgaactcaag gcgcgcttcg acgaggaggc caacatcgaa
     5161 tgggcgcagc gccttgagca cgcgggcgtc cacgtcgtct atggcgtgct cggcctcaag
     5221 acgcacagca aggtggcgct cgtcgtccgc cgcgagggcg aggagctgcg ccgctacgtc
     5281 cacatcgcca ccggcaacta caacccgttc acctcgaccg tctacacaga cctcggactc
     5341 ttgaccgcca acgaggagat cggcgccgac gccaccgacc tcttcaactt tctgacgggc
     5401 ttctcgcggc agaagactta ccgccgcctg ctcgtcgcgc ccgtgaattt gcgcgagggg
     5461 atgctcgccc tcatcgagcg cgaggccgag cacgcgcggc agggacgccc cgcgcgcatc
     5521 gccgccaaga tcaaccggct ggccgacctt cagatcatcc gcgcgctcta cgccgcctcg
     5581 caggccggcg tcaccatcga cctcgtcgtg cgcggcgtct gcatgctgcg gcccggcgtg
     5641 ccggggctgt ccgacacggt gcgcgtgcgt agcatcgtcg gccgcttcct tgagcatagc
     5701 cgcgtcttct acttcgagaa cggcggcgaa gaggaagtct acatcggcag ctccgactgg
     5761 atgtcgcgca acttagaccg ccgcgtcgaa gtcgtcaccc ccgtactcga cccgagcctg
     5821 cgccgctacc tgaaggacgt ggtgctggcg gcttatctgc gcgacaacgt gaaagcgcgc
     5881 gtgcttcagc ccgacggcaa ttacgtccgg ctgcgccccg ccgccggcga agagggcttc
     5941 agcagtcaga cccacttcga gggcgcggtg agcatgaaca tttagccacg cgagcatccc
     6001 ccgctcgctt cgaccgcgac tctgcaacgc cgcgcgccgc gccggccgct gtttcgtcgg
     6061 tcttaaatct cccccgcaaa gagtctcgct tttttgtttg ccgccggcag tgcgaaggcg
     6121 tgcgtgaatg tttatcgtga atgcttattc ggaggaaaca gttatgtaca cacttggtca
     6181 aactcgccgg cgctccgcct ttgcgctcgc gctcgttctg ggcctttcga ctctgaccgc
     6241 ctcggccgtc cagaaagacg acaagaagaa ggagccgatg ccgcagggca cggccgtcct
     6301 atggcaagac cccggcgaca tctccgcgcg caacctgctc gcggggccgg ggcctgacat
     6361 gaagcccgat ttgtcgagcg tcaccttcgt caaggacgag atgggcggct actcgccgaa
     6421 gttccgcgtg acggacggcg cgggcaagac gtgggtggcg aagtttggca aggaggcgca
     6481 gcccgagacg gccgccgtgc gtctggcgtg ggccgtcggt tacgtgacgg aagtcgcgca
     6541 tctcgtcccg tgcgtccaga ttaagaatgc cccggagccg cgcaaggagg tcgagcgatg
     6601 cgacggcaag ggcttcgcca acgtccgctt cgaggcgcgg cccgatgagt ggaagcgtct
     6661 ggacaactgg tcgtggaagg agaacccgtt cgccggccag aaggagtttc agggtctcat
     6721 cgtgatgatg ggcctcatca acaactggga cttgaaggac accaacaaca agatcgtcta
     6781 cgtgcccggc gccgactcgg ccgccggcga gttgcgctac atcatcagcg acctcggcgc
     6841 gaccttcggc aagaccggca acttcatcac acacgaccgc aacaacccga agaacttcgc
     6901 caagtcgaaa ttcatcgagc gcgtcgaggg cggtcgcgtt cgtttctcct acgacggcaa
     6961 gaacacgggc ctcttcgacg acatcagcgt cgagaacgcg cgctggatcg gcgaccaact
     7021 cgcgcgtctc tccgacaagc agatcgagga cgccttccgc gccgccaact acgccggcgc
     7081 cgacctccag acgctcgtcg ccgaagtgcg ctcgcgcatc aacgaactaa cggccctgcc
     7141 cgcgcccgcc gccaccgccg gcacgcccgg ctccggcact cccgacaaga ggtaacaagc
     7201 aatgagatac ccgcgccaga ttcacagact cgtctccctc gccctgctca cagttctcgc
     7261 cctctcgtcg ctccagcccg ccacggccgc gccgcagaag gaggcgaagg gaaagcagga
     7321 ggacaggaaa gagaaaaagg agacgccgcg cggcgccccc gtcctgtggc gcgcgcccgc
     7381 cgacatcgta acgcgcgacc tctatctggg gccgggcggc gagtcgatgc ggcccgacct
     7441 gcgtaaagtc accttcctca aggacgagac gggcggcggc tactcgacca agtttcgcgt
     7501 gcgcgacgcc tccggccacg agtgggtggc gaaggtcggc aaggaggcgc aatccgagac
     7561 ggcggccgtg cgtttgattt gggccgtcgg ctacgtgacc gaggtcgtct atctcgtccc
     7621 gcgtctcaac gtcgagggca agggcgcgtt cgacaacgtc aggctcgaag cgcggcccga
     7681 cagcgtcaag cgcctcgacg agtggaagtg ggccgacaac ccgttcgagg ggacgaagga
     7741 gctacagggc ctcatcgtga tgatggcgct cctcaacaac tgggacatca aggacaccaa
     7801 caacaaggtg ctctacgtcc gcgatgaggc gacggggcgc ggcgagtcgc gttacgtcgt
     7861 cagcgacctc ggcgccacct tcgggcgcgt caaggtgaac gtgcccggcg tctggcggct
     7921 tcagcgcagc cgcaacaacc ccgaggacta ctggaaagac ccgttccttg aggacgtgaa
     7981 gaacggtcag gtcttcttct tctacaaggg caagcggcag gacttgttcg acgacattcg
     8041 catcgacgag gcgcgctgga tagcggccct gctctcgaag ctgagcgacc ggcagatcag
     8101 cgacgccttc cgcgccgcca actacacgcc ggccgagata cgctcgctga ccgaggccgt
     8161 gcgcgcgcgc atcaacgagt tggcctccat ccgcggcaac gcacccttgg cgagtaatcc
     8221 gtagagggca taaaacagta ccgcgagcgg aagcctcgcg ggtctggctg agcgaggctg
     8281 gcctctcagg ataggcgtac ccgctcgcta ccgctcgcgg tactgtttcc gcgcctgtgc
     8341 ggtgagtcga tttcggttat actcagcttc ctacgaacca acctcgcgcg ataaaaccat
     8401 tcaccggagg actgaaactt tgtcgagcgc gacgatcatc cgtagactct gctcctcgct
     8461 tctcatcatc atccttgcct tcacgagcgc ccccctcgcc gcgcaggaca agtcgaagaa
     8521 caagaagaaa aagcccacgc cgcgcggcac gcccgtcctg tggcgcgcgc ccgccgacat
     8581 cgagtcgcgc gacctcgcgc tcggccccgg cggcgccgag atgcgtccga acctgcgccg
     8641 catcacgttc ctcaagaagc aggaaggcgg ctggtcaacc aagttccgcg tgcgcgacgc
     8701 ctcgggacgc gagtgggtcg ccaaggtcgg caaggaagcg cagtccgaga cggcggccgt
     8761 gcgtctggtg tgggccgccg gctacctgac cgagatcaac tacctcgccc catgcgtccg
     8821 catcgaaggc gcgcccgcgc ccggcaagga cgacgagcaa tgtgaaggcg ggggcttcgc
     8881 caacgtccgc ttcgaggcgc ggccgaaagg tgtcaagcgc ctggacccgt gggagtggaa
     8941 gaagaacccg ttcaagggga cgcgcgagct tcagggtctc atcgtcctga tgggcctcat
     9001 caacaactgg gacttgaagg atacgaacaa cgtcgtcctc gccgcgccgg gcgcgcgggg
     9061 ccgcaccgag ctgcaatacg tcatcagcga cctcggagcc accttcggca agaccgggag
     9121 cctgccgctc ttctggcgcg tcacgcgcag ccgcaacgac cccgagggtt ttagcaaaga
     9181 caagttcgtt gacgaggtcg aggacggcaa ggtcagtttc cacttcacgg gaaagaaccg
     9241 cggtctcttc gaggacatca cggtgcgcga cgcccgctgg atcggcggct ggctctcgcg
     9301 cctgagtgag cgccagatcg gcgacgcctt ccgcgccgcc aactacgccc ccgacgaggt
     9361 gcaaatgctc accgacgccg tgcgccgccg cgtcggcgaa ctcacggccc tgcgcggccg
     9421 gggcgccgag tccggcgacg ccggcgggcg ccggaggtga cagcggcgct cattcgagtc
     9481 tatgctcgtt cggcgcgcaa ggttcaatcc agttgagagg gaaacgttta atgaagaggc
     9541 gtgatcggtc agtgtttctg ttggtggggc tggcggcggc cgtgctcggc ttcggcatcg
     9601 tcgcctacat gctctccgac cgttcggccg cgcccgcggg gccggcggac gcgacgacga
     9661 cgagcgcgcc cacgcccgcg ccgacaactc agccgacggc ggccgcgacc acggccgacg
     9721 cgcgacgcgc gcccacgccc accggcgccg agggcacgcc cttcgagtat ctcttctctg
     9781 aggacgccca gggcgcgcct gcgcgccagg agacgctctc ggccagagtg ttgcggatcg
     9841 tctggcggct ggcgctggcg gcgctcctcg ccgcggccct cgccttccgc ccgcgccgag
     9901 acctcgccgc acaccagcgc aacctctacg tggcgcagac gcaaatcctt ttggccgtcg
     9961 tcgcctcctc gctgatgatg atcgtcggcg acaacgccgc ccgcgccttc ggcatcttcg
    10021 ccgccgtctc gctcgtccgt ttccgcacga acatccgcga ccccaaagag atcacggtgc
    10081 tcctcatcag cctcgcgctc gggctggcga cgggcgtcgg ccgctgggag ctggccatca
    10141 tcctcacgct gttcgtcttc ccgctgctct ggttgctgga gcgccgcgag tccgaggagg
    10201 tcttccgcac gatggagctg accgtcaaga cgcgcaacac cgaggcgacg caggagatgc
    10261 tccgcgaaat cttcgaccgc tacgaaatga gcgccgagat gcgccagatc gacccgcccg
    10321 acgaggccga acccgtcggc tgcatcatgt accgcgtcag catgaacatg aacgtcagca
    10381 ccgacgagat caacgagcgc atcctcacca tcgaccccga aaacgtcgaa ggcatcgagt
    10441 gggagcagaa gaagaacacc ggctacgtct acaactagct cttagctgtt agctaaaagc
    10501 ttttatgagc attgaaatca tttgccgtgc atcctggagg cgtctggctt cgtcaaagct
    10561 gcggtagaag tcccgcccga ggagtccgaa ggccatgaga acgctgacca cactgttgct
    10621 gctcgcctcg ctcgtcgtca ccgccgcgtt tgcgccggcc ggcctcgcgc agtcgcgcgc
    10681 ccggcgcgtc ggacagtcgc aacccacagc aacgacgccc gcgccgcagc cgcgcacgcc
    10741 ttcggcgcag acgcccggca cggccgacgg cgcgcgcgac cggcaggtgc aggaagaggt
    10801 cggcgacgac gaggtcgtgc gcgtcgaatc cacactcgtc accatccccg tcagcgtcct
    10861 cgaccgcgac ggccgcttca tccccgcgct cggcaaggac gacttccgca tcatcgaaga
    10921 cggcgtcgag caacaggtgg cctacttcgc caccgtcgaa tcgcccttca cggtcgcgct
    10981 cgtcttagac acctccggct ccacccagtt tcgcctcgaa gacatccagt cggccgccac
    11041 cgccttcgtc gatcagctcc gcccggatga ccgcgtcctc gtcgtctcct tcgacgacga
    11101 gattcgcttc ctctccgaag cgacgagcga ccgccgccaa ctgcgcgacg ccatccgccg
    11161 cacgcggccc ggcggcggca cgcgtctcta cgacgccttc gacgccgtca tcaagcagcg
    11221 cctcgccagc gtcgagggac gcaaggccat cgtcctcttc accgacggcg tggacacctc
    11281 cagcaagcgc gcctcctact ccggcaacat gcgcgacgcc gaggaactcg acgccctcgt
    11341 ctaccccgtg cagtacgaca cctacggcag ccagcagtcg ggcggcggag gctggcccgg
    11401 cggcggtggc ggcggcggcg gttcgatacc ccggacgggt ggggttggcg acatcctgat
    11461 aggcatcctc ggcgggattg gcagcaggcg cggcggcggc ggccccgtga ccatcggcgg
    11521 cggcggcggc gcgacgcgcg aggactacgc gcgcgcgggc cgctacctgc gcgagctggc
    11581 cactctgacc ggcgggcgac actatcaggc cgacgacctc agctatctcc ggcaagcctt
    11641 ctccaacatc gccgaggagc tgcgccgaca gtacagcctc ggctactatc cgtcgaagca
    11701 ggcgcaggcc tccgagcgcc gccagatcaa ggtgcgcgtc aagcgcccca acctcgtcgt
    11761 gcgcgcccgc gacagctaca tctaccgacc gggcggctcc acgacgacgg cgcagggcga
    11821 ccccaacaga cagccgtccc agacgccgcc cgagctgcgc cgcaagcagt tcgcgaaggc
    11881 tgacgcgccc atcaggtgag acggcaaaaa gtgtcagcgc aaaagcgtaa ggcccctccg
    11941 gcgcatgtcc gaaggggcct ttaatctttc gtcctccgtc tcccgctaaa gcgtggtgcc
    12001 gtcggggacg gtcgcgtcct tcgggacgac gatgatgccc tcgcggatgt ggacgctgcc
    12061 gtccggggcg tcgtactctc tcaggcccgc ctcgttgacg aggcggacgc ccgcgccgat
    12121 gcgcgcgttc ttgtcgatga tggcgttgcg gatgacggcc ccctcgccga tgccgacgcg
    12181 cggcgacccg gccgccttct ccgtcctcat ctcttcgagc gactgatacc agtcagcgcc
    12241 catcaggtaa gagttctcga tgtgcgcgcc gtggtcgatg cgcgtgcgca gaccgatgat
    12301 ggagcgcgtg atgtgcgcgc ggttgatgat gcagccctcg gtgatgttcg actgttgaat
    12361 gtggctgttg ttgatcttcg acggcggcag gtagcgcgcg cgcgtgtaga cgggcgcttt
    12421 ggagttgaag aagttgaact tcgggatggt cgccgtcatg tcgaggttgg cggcgtggaa
    12481 ggccgagatg ctcccgatgt cctcccagta gtcgtcgaag acgaacgcct gcacgttgcc
    12541 gcgctcgatg gccgccggga tgatctgctt gccgaagtcc acccaggcgg tgttggcggc
    12601 cagcaactct tcgagcttct tgaagtcgaa gatgtagatg cccatcgagg cgaggaacgg
    12661 gcggcgctcc gcctcgttgg ggtcgaggcc gaagcgcgac gtgtccacgc gcatctcttc
    12721 gagcgcctga cctttgggct tttcgcgaaa ctccgtgatg cgcccctcag cgtccgcctt
    12781 gagcaagccg aactcctcgg ccgcctcgcg cacgaagggg atgacggaga ccgtcaggtc
    12841 ggcgcccgtc tcgcggtgac gcgcgaggaa gtcgcgatag tccatccggt agaggtggtc
    12901 gccggagagg atgagcaggg tgtcgatgcc ccagtcgcgg atgtgcggga gcacctgtcg
    12961 gacggcgtcg gccgtgccct gaaaccactg cggactctcg ggcgtctgct cggcggcgag
    13021 gatgtccacg aagccgctgg agaagtgtga gaagcgatag gtctgcgcga cgtggcggtt
    13081 gagcgacgcc gagttgtact gcgtgaggac gaagattttg agcacgtccg agttgatgca
    13141 gttcgagatt ggaatatcga cgagccggta cttgccgcca agcggcacgg ccggcttcga
    13201 gcgcatcttc gtcagcggga acagacgcgt gccagcgccg cccccgagaa tcaccgccag
    13261 cacgtcatgc ttatccgccg gcataacctt cccccacaat ctttgtttga ttactgtgcc
    13321 ccagaggata gacgcaaagc ggcgcgcgtt caaggagacg gccgagccgg caaactttca
    13381 caacgttttc ccgctacctt gaccgacgcc gacgaaagcc acaaaggaga actcaaatgt
    13441 cactgttggc ggcactgggc agcggtcttg cgggcgcctg cgccctcacg gccatccacg
    13501 aaaccgcgcg ccgcgtgctt cccgatgccc cgcgcatgga cgtactcggg atgcgcgcca
    13561 tctcccgcgc cttccgcgcg gccgacgccg aggagccgcc gcgcgcacaa ctccacgacc
    13621 tcgccctcgc cggcgacatc ctctccaact cgctctacta cagtctggta gctctgggcg
    13681 ggccgaaggg cgcggttgcc tgtggggcca cgctcgggct ggcggcgggc ctcggagccg
    13741 tggccctgcc cgcgccgctc ggcctcggcg acgggccgag cgcgcgcaca aacgagacaa
    13801 aggcgatgac ggtcgcctgg tatctggcgg gagggctggc ggcgggactg gcttacagcc
    13861 ggctcacgcg aaaacggtaa ggcgttatca agactgtagg ggcgggcctc gtccccgccc
    13921 ctacaatttc tggtcgccga acgccccgcg ctcgcgagtt caataggccg cgactgaggc
    13981 gttggtttgg acgcccgccg cctcctgcgc gatcttgacg ccgacgctcg cgccgatgcg
    14041 cgtcgcgccc gcttcgagca tcgcccgcgc gtcgtcaatc cccttgacgc cgcccgccgc
    14101 cttgacgccc atcccggcgc ccaccgtgcg acgcatcagc gccacgtccg cgaccgtcgc
    14161 gccgcccttc gcaaaccccg tcgaagtctt gacgaaatcg gcgcccgcgc gcttcgccgc
    14221 gagacacgcc cgcaccttct cgtcatcgtt taagagcgcc gcctcgatga tcaccttgac
    14281 cagcacgccg tgctcgtgcg acgcctcgac caccgagcgg atgtcgtgct cgacggcgca
    14341 gtcgtcgccg ctcttcagcg ccccgacgtt aatcaccatg tccacctcgc gcgccccgtc
    14401 catgatggcg cggcgcgcct cgtaagcctt cacgtccgca agcgtcgcgc cgagcgggaa
    14461 gccgaccacc gtgcagaccg gcacactcgt cccgcgcaga ttgcagacgg ccgcgcgcac
    14521 ccacgacggg ttcacacaca cgctcgcaaa acgatactgc gccgcctccg cgcacaactt
    14581 gcgaatgtcc tcgtccgtcg cctcgggctt caagagcgtg tgatcgacga acccggccca
    14641 atcgcgcgcc gtccccgtct gccccagcac cagcccgatg cgactggcgc ccgcgtccac
    14701 gatccgctgc atccgctccg ggcacctgtc gaaacactcc gacgagcacc cgcacatcgc
    14761 cgcctgccca tcgccgtcgc cattgagacg cgcgacgatc acatcggtaa tctgctcgac
    14821 caagcgttcc aagtcgccgt tgtttgtgtg tgacatctga ctcggcgctc ctcgacttac
    14881 ttccgctgct tgacgaaaga ctccctgacc gcgatgcgct ccgcaggtat caacaccacc
    14941 cggccaccct cccacgatgc aactgtgtta cccgcgctct tatgcactgc gagcgcgccg
    15001 cgcacagcct tatgcacggc cgcgtcaaga gcctgctccc tgacgatgaa caactccttc
    15061 acgtcttttg actcgactct cattttgcta cctcgctcaa acgctgccac agaccttcat
    15121 cgtgcacctt caacgcggac gccccgtggc ccgcggcgac tctaacgggg ctgccagaga
    15181 cagaattatc atacaccacc caggaatcgg ccaatgaccg gtacaggtga aagaagtttc
    15241 gcgcggccct cacgtagcgc ctgcgaacga tatgctcggg cacgtcgtgg ccgccgaggc
    15301 ggacgcgctc cctgacgcgc tcgacggcga ggtcggggct acgcagccac atgaaaaaga
    15361 ggtggaagtc gtagcccttc tgcttcagac gcttgagaaa ggcggcgtag gagcgcgtcg
    15421 ccagtgtggt ctcaaaggcg aagctgcggc gcgcgtcggc gaggtcacgc agccgccgaa
    15481 gcatgatccg gcccgcctgc gcggtcgcgc cctgcgggtt gaacgcggac aggccgcggg
    15541 cgatgtcatc tgcgttgacg aactcgctta ggccgagttc gtcgcggagc aggaatggag
    15601 cgagagtggt cttccccgca ccattaggcc ctgcgatgac gactacttgc gggcgcggtt
    15661 cgctcatcgc tcagcttcta tactgtcgct cgacggcctc gatcttggcg acgcgtttct
    15721 tgtgccgctc ctcgctaatt tcagtcgcga gccagacgcg gacgatgtcg cgcatctcgt
    15781 cgagggtgat ggcgccgctc cccagggtga ggacgttggc gtcgttgtgc tcgcgggagt
    15841 tgcgggcgac cttaggggag tagcaggcgg cggcgcggac gccgggcact ttgttggcgg
    15901 tcatggcgga gccgacgccc gcgccgtcca cgacgatgcc gaggtcggcc tcgccctcgg
    15961 agacggcgcg cgccacggcg tgcgcgaagt cggggtaatc gaccgcgtct gtcgagtcgg
    16021 tgccgaagtc gcgcgtctgg tggccgagtt cggcgaggag ttttttcagc tcctccttca
    16081 tcggatagcc gccgtggtcg gcgccgacgg cgacaatctt cagcgcccgg ctcccccgcc
    16141 gcgacacgcg gcggacgagt tcgatgcgcc gctcgcggac gatgtcggcg gccagaggcg
    16201 tcaggcgcgc gccctccgcg acgcgcaaac gtgcgccctc ctccagcccg cgcacgtcgt
    16261 cttccgtgat gaccatcttc gacgattcgt cgcgcacgaa cgacggcccc tcgctgccat
    16321 cgcccgtcgg tgccgtctgg atgatgcgcg cgcgcgtcgt tccagaatcc gcacccgcac
    16381 ggcttaatga cggcgcggac tcagacggcc tcgccgaggc ggcgcctgcc gtcgtcgcat
    16441 cctccgccgg aagcgcgttc tccagcacgt ctcttacgag cgcccgcacg cgctcgcgcg
    16501 tcgattgtgt gtcgtcggcc ataaggcaaa aagtgagcag tgagcagtaa gcagtgagca
    16561 gtaaaaacaa gttcggcgat gagcggcaag ctacgtaagc gtccaactgc ttactgctta
    16621 ctgctcactg ctcactatgg ccgacgacac acaatcgacg cgcgagcgcg tgcgggcgct
    16681 cgtgagagac gtgctggaga acgcgctccc ggcggaggat gcgacgacgg caggcgcc
//
LOCUS       DASVES010000494         8612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1390604, whole genome shotgun sequence.
ACCESSION   DASVES010000494 DASVES010000000
VERSION     DASVES010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390604"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..3910)
                     /locus_tag="VF240_20855"
     CDS             complement(71..3910)
                     /locus_tag="VF240_20855"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7177722.1"
                     /translation="MKLNRLPRLFRVAMLSLSLAAVAAAQGTTSRVTGVVLDPQGAAV
                     QNATVTLTSEATTVAFTTQTTSTGAYTFDSVQAGTYTLAVEQTGFKKFVSPNNAVNVN
                     QPATIDVTLEAGSIAETVTVDAAVELVQTATSGNFGNTVEERPLEALPIVGNRGRNPL
                     EFINFQPGVVPNANTGGGVHVHGARDRAFNFTLDGIDINETSAGGSNFTPIRTNPDAI
                     TQFQVVTSNFTADNGRSSGAQVSLVTKSGTNEFHGNLFEFYQTPRFHANEYANTINTR
                     LVGGERVPTDKPQFVQHIFGGSVGGPVYLPRFGEGGPVVYSGKNKTFFFVNMQLLRTT
                     QAFFVNRTVYTEQARAGIFRYALGRANANATVTGANTRVIDENGVPFLPTCSATVTAN
                     CIRSFNVVGNPNIPLTPDATTTRLIGLTPLPNNFTLGDGLNTAGFSFVAPQSERQYDF
                     TTKIDHNFNDRNSMFVRYSRGSQTTSCDNVNGGLPRFPGFPCYVDTTRTPRNLAVNYR
                     ATITPTTVNELVVGFNRFTFGFANPDPNATTNPPIILNLPTDPLNFEAPLNNARQITT
                     FQIVDNLSHQHGAHTLKFGTNLRFQKHVDDRTSVAGFLTVQTVRLGTGDNAVPEGFGT
                     RAAALPGATTANPNAGINTTDRGRLDSFINDLLGRIGRIEQSFVAADDSTFAPAGTRF
                     NYKAEYPEYDFYGQDTWKARPNLTFDYGLRWEMRLSPRAPDDIILRPSMPVRFGETPT
                     NQISFTEGKLYEDSLKQLAPTVGFAWDPFGDGKTSVRGNYRLAWDRTNTFVFSSFIFQ
                     SAPGLTRAITLSANNSAFGGTANALVRNGLPTLSANDTTPTAFRTPPAFSQNTLNVVD
                     PSLTYPRTHQYGFSVQREVGWNSVLEVNYIGRQGRKLFGGYDVNQVDIFNNGFLDAFR
                     QLQDPATRPGVITNPNFLINRLLAGDSRLTGGETGSQFLLRQAVGGNVTTPSGAIATN
                     IVDAGSAAQAAFLARLNNRFVTNGFSPFFFQPFPQFTGAVNVIDNNDRSRYNALEVQL
                     SRRFTRGVGFQVSYTLAKSEDTRSFDPALALANRGTAQSGANTPFDIRNRELNYARSD
                     FDRRHAIQGYFTWDLPFGQGRRWMSDAGPVADRILGGFELAGIVRLYSGRPFTIYSGI
                     NSVSQVNQSPVSCNGCTPDMGRIVLEGGRNFIFTEEQRAQFFAPAAGELGNTGRNFFT
                     GPRLFTLDLTLKKRIRFDESRNVEFRIEAQNATNTPSFGVPSDANLVASSASFGFVGG
                     NVIGGGRKMQLAVKFNF"
     gene            complement(4573..5598)
                     /locus_tag="VF240_20860"
     CDS             complement(4573..5598)
                     /locus_tag="VF240_20860"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177723.1"
                     /translation="MSSLKTYNLCLLGFGNVGRALARLLVEKRDEMRDQYGIEWRVTG
                     VATRRMGWLIAPEGFDDAELESGELRARVGSEPKDVGEWLDAARTEVLFENTSLDTHA
                     GEPAVSHIRAALERGAHAVTANKGPVVHAFEELDALARKRGRRFMFESAVADCLPIFS
                     LFRASLPAARLLSVRGLLNATSSVILEAIEEGATFDEGVRRAQELGLAETDPSNDVDG
                     WDAAVKICALANVLMDARLKLGDVRREGIRGLDAEDVRAAKRAGAPLRIVARARRGAD
                     GRLHASVGPQPLAPEDPFSKLSGSSMAARFELDVVPALTLIEDGADNRSTAYGCLADF
                     INAVREM"
     gene            complement(5694..7007)
                     /locus_tag="VF240_20865"
     CDS             complement(5694..7007)
                     /locus_tag="VF240_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177724.1"
                     /translation="MHRKPPTFVLFLSLLFAFSFSAHAADQVGPLDLQGEIGGAPYRI
                     KVPAAWNGTLLVFAHGYRDKADHPGEVDNRTADASPNPALDPALLAQGYALAGSAYRD
                     NGWAVEEGVQDMKNLVNYFRAYVTKPDRTIIWGFSLGSFITLKSMEHFGGVYDGAIAA
                     CAGGAGATRTWDSSADLWLAYDTVFGAPSTWGEVGDARDDIDFETDVRPKLLAEIGNP
                     LNFGKFEFVRLVIGNPGRGIQPPPPPFFYPGWLLTDMFFATEARAELERRAGGPVVQN
                     LNHTYSLTPAEKAYLQSFGVSPAVADAWLAAMNARTNVSAPNHSRNYVERNADYSGKI
                     KHPVLTLHTIVDGLVVVTQESAYRETVEAAGRADRLFQVYTSGSQLGVVPGQIGHCDF
                     TPQQLFASVAAIENWVKTGVRPTPASFPAALGFVPNFTPPPFPQP"
     gene            complement(7148..7651)
                     /locus_tag="VF240_20870"
     CDS             complement(7148..7651)
                     /locus_tag="VF240_20870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7177725.1"
                     /translation="MQDTEYTARLATRDDAALIVEFNLAMARETESLELSPATLASGV
                     RALYDNPQFGFYVMAEARGPGDGAVECAGSLMITYEWSDWRDGLFWWVQSVYVRAEHR
                     RRGVYTVLYEFVKRLAAADPRVRGFRLYVERENVAAQHTYERLGMSETHYKMYEAPLE
                     RDAAEKS"
     gene            complement(7664..>8612)
                     /locus_tag="VF240_20875"
     CDS             complement(7664..>8612)
                     /locus_tag="VF240_20875"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX7177726.1"
                     /translation="LKAKDSAEVTHAEALAKQTKAPPRYSESALLGAMETAGRKVEDE
                     ELRLAMKEAGLGTPATRANIIETLLKREYVRREKKSLVATEKGAALIRMLPYPLLKSA
                     ELTGMWEQKLSRMTRGEYAFDTFMGEVKGIVVELVAQIAGSQVEGAPASARSAAPRPE
                     GAHDCPKCKAEERAGFLVERTSTAGKFLACSLGKEACGFLSDTPKNAKQRKALFETKC
                     AACGGAVRLRLPREKGRNASLACSRYPACQAVRWFDEGGALQEPRPAPETGPECALCG
                     TPTVKRGPTSAGSHFWSCPRWRSDGTGCNSKPVWINERG"
BASE COUNT         1350 a         2609 c         3120 g         1533 t
ORIGIN      
        1 caaaattcgc ggccggggaa acggggcgcg ggcgcgactc caaatacttg acgcgcccgc
       61 gcccgctcag ttagaagttg aacttcaccg ccagttgcat cttgcgcccg ccgccgatga
      121 cgttgccgcc gacgaacccg aagctggcgc tggaggcgac gaggttggcg tccgaaggca
      181 cgccgaacga gggcgtattt gtcgcgttct gagcctcgat gcggaactcc acgttgcgac
      241 tctcgtcgaa gcggatgcgc ttcttgagcg tcaggtcgag cgtgaagagg cgtgggccgg
      301 tgaagaagtt gcggccggtg ttgccaagtt cgcccgcggc cggggcgaag aactgggcgc
      361 gctgctcctc ggtgaagatg aagttgcggc cgccctccag cacgatcctg cccatgtcgg
      421 gcgtgcagcc gttgcagctc acgggcgact ggttgacctg cgagacggag ttgatgcccg
      481 agtagattgt gaagggccgt ccagagtaca aacgcacgat accggccagc tcgaaaccgc
      541 cgaggatgcg gtcggccacg gggccggcgt cgctcatcca gcgcctcccc tgtccgaatg
      601 gcagatccca tgtgaagtag ccctgaatgg catggcggcg gtcgaagtcc gagcgagcgt
      661 agttaagctc gcggttgcgg atgtcgaagg gcgtgttggc ccccgactgt gcggtgccgc
      721 ggttggcgag ggcgagggcc gggtcgaagg agcgcgtgtc ctcactcttg gcgagcgtat
      781 agctgacctg gaagccgacg ccgcgcgtga agcggcggct gagctgaacc tccagcgcgt
      841 tgtagcgcga gcggtcgttg ttgtcgatga cgttgacggc gcccgtgaac tgcgggaatg
      901 gttggaagaa gaacgggctg aagccgttgg tcacgaagcg gttgttaagc ctggcgagga
      961 aggcggcctg cgcggcgctg ccggcgtcca cgatattcgt cgcgatggcg ccgctcggcg
     1021 tagttacgtt gccgcccacg gcctgacgca gcaaaaactg cgagccggtt tcgccgccgg
     1081 tcaggcgcga gtcgccggcg aggagtcggt tgatgaggaa gttcgggttg gtgatgacgc
     1141 cggggcgcgt cgccgggtcc tgaagttgac ggaaggcgtc gaggaagccg ttgttgaaga
     1201 tgtccacctg attaacgtcg tagccgccga agagcttgcg gccctgccgg ccgatgtagt
     1261 tgacctccaa caccgagttc cagccgactt cgcgctggac gctgaagccg tactggtgcg
     1321 tgcgcgggta ggtgagcgag gggtcaacca cgttcagcgt gttctgggag aaggccggcg
     1381 gcgtgcggaa cgcggtcggc gtcgtgtcgt tggccgaaag cgtgggcagc ccgttgcgca
     1441 ccagcgcgtt ggccgtgccg ccgaaggcgg agttgttggc cgacagggtg atggcgcgcg
     1501 tcagccccgg cgcgctctgg aagatgaacg aggagaagac gaacgtgttc gtgcgatccc
     1561 aggcgagacg gtagttgccg cgcaccgagg tcttgccgtc gccgaacgga tcccaggcga
     1621 agccgacggt cggcgcgagc tgcttcaacg agtcctcgta gagcttgccc tccgtaaagc
     1681 tgatctggtt ggtcggcgtc tcgccgaagc gaacgggcat ggaggggcgc aggatgatgt
     1741 cgtcgggggc gcgcgggctg aggcgcatct cccagcggag gccgtagtcg aacgtcaggt
     1801 tcgggcgtgc cttccaggtg tcctgcccgt agaagtcata ctcggggtac tcggccttgt
     1861 agttgaagcg cgtgcccgct ggggcgaagg tcgagtcgtc ggcggcgacg aacgactgct
     1921 cgatgcgtcc gatgcgtccg aggaggtcgt tgatgaagct gtcgagccgc ccgcggtcgg
     1981 tggtgttgat gccggcgttg gggttcgcgg tggtggcccc cggcaacgcc gcagcacgcg
     2041 tgccgaagcc ttccggcacg gcgttgtcgc cggtgccgag cctgacggtc tgcaccgtga
     2101 ggaagccggc gaccgaggtg cggtcgtcaa cgtgcttctg gaagcgcagg ttggtgccga
     2161 acttcagcgt gtgcgccccg tgctggtgcg agaggttatc gacgatctgg aaggtcgtga
     2221 tctggcgcgc gttgttgagc ggcgcctcga agttgagcgg gtcggtcggg aggttgagga
     2281 tgatcggcgg gttggtggtc gcgttcgggt cggggttggc gaagccgaag gtgaagcggt
     2341 tgaagccgac gacgagttcg ttgacggtcg tcggcgtgat cgtcgcgcgg tagttgacgg
     2401 cgaggttgcg cggcgtgcgc gtggtgtcca cgtagcaggg gaagccgggg aagcgcggca
     2461 gcccgccgtt gacgttgtcg cagctcgtgg tttgcgaccc gcgcgagtag cggacgaaca
     2521 tcgagttgcg gtcgttgaag ttgtggtcaa tcttggtcgt gaagtcgtac tgccgctcgg
     2581 actgcggggc gacgaagctg aagccggctg tgttgaggcc gtcgccgagg gtgaaattgt
     2641 tcggcagcgg cgtcagcccg atgaggcgcg ttgtcgtcgc gtcgggcgtg agcgggatgt
     2701 tcgggttgcc cacgacgtta aagcttctga tgcagttcgc cgtcacggtg gccgagcagg
     2761 tgggcaggaa ggggacgccg ttctcgtcga tgacgcgggt gttggcgccc gtcaccgtgg
     2821 cgttggcgtt agcgcggccc agggcatagc ggaagatgcc ggcgcgcgcc tgctcggtgt
     2881 agaccgtgcg gttgacgaag aaagcctgag tcgtgcgcag cagttgcatg ttgacgaaga
     2941 agaaggtttt gttcttgccg ctgtagacta cggggccgcc ctcgccgaaa cgcgggaggt
     3001 agacggggcc gccgacggag ccgccgaaga tgtgctgcac gaactgcggc ttgtcggtcg
     3061 gcacgcgctc gccacccacg aggcgcgtgt tgatggtgtt ggcgtactcg ttggcgtgga
     3121 agcgcggcgt ctggtagaac tcgaagaggt tgccgtggaa ctcgttggtg cccgacttcg
     3181 tcaccagaga gacctgcgcg cccgaggagc ggccgttgtc ggccgtgaag ttgctggtca
     3241 caacctgaaa ctgcgtgatc gcgtcggggt tggttctgat cggagtgaag ttcgagccgc
     3301 cggcgctcgt ctcgttgatg tcgatgccgt caagcgtgaa gttgaaggcg cggtcgcgcg
     3361 cgccgtggac gtggacgccg ccgcccgtgt tggcgttcgg caccacgccg ggctggaagt
     3421 tgatgaactc cagcggattc cggccgcggt tgccgacgat gggtagcgcc tccagtggcc
     3481 gctcctccac cgtgttgccg aagttgccgg aggtggccgt ctggacgagt tcgacggccg
     3541 catccaccgt cacggtctcg gcgatgctcc cggcttcgag cgtcacgtcg atggtcgccg
     3601 gctggttgac gttgacggcg ttgttcggcg agacgaactt cttgaagccg gtctgctcga
     3661 cggcgagcgt gtaggtgccc gcctgcacgg agtcgaaggt gtaggcgccg gtcgaggtcg
     3721 tctgggtggt gaaggcgacg gtggtcgcct cgctcgtcag cgtgacagtc gcgttctgga
     3781 cggccgcgcc ctgcgggtcg agcacgacgc cggtcacgcg cgaggtcgtt ccctgcgcgg
     3841 cggccacggc ggccagcgag agcgagagca ttgccacacg gaatagacgg gggagacggt
     3901 tgagtttcat cggaaggctc ctcgcggttg aagatggtta acgacagtac ttcaaaaaaa
     3961 gattacgcac ggataagcgg gactggcgcg gcgacgcgga ctgcgggcgc gcgtggctcg
     4021 cgctcggttg cgtcacgggc gctggcggtc gaacccctcg cgcccgaaga ggcaagcatg
     4081 ttcgcggcgg ggtcacgccg taggaaaaga acccgctgtg tgtcagttac tgcaagtgtg
     4141 gctgggcagc gccctctgct aaagcgtctg cttttatttt aggtttacgg gacgccataa
     4201 gggaaactaa aaattcttat cactgagata acagcttctg atgtatcgag aagacccgcc
     4261 gtcggccccc gacgcgtccg ggaagtgtcg gatcgaacgg cgcgtcgggc cacgggcgcg
     4321 cgtcggccga ttgagttttt tattatcacg caatcattgt tccagcaagc cggtagaact
     4381 aaagagcggc tttcgcgcaa ccgcctccgc tccgcgttac gaaaaaatgg atgaccctat
     4441 tgttttttgg ataatcggcg actcgcaggg aacacggatt ggacggaggc gacggatgtc
     4501 cacggattac atctgtgtca atccgtcgca tccgtcttat ccgtgttctg tcttcatgcc
     4561 ggttcgggtc gtctacattt cccgcacggc gttgatgaag tcggcgaggc atccgtaggc
     4621 ggtgctgcga ttgtccgcgc cgtcctcgat gagcgtcagg gccggcacca cgtcgagttc
     4681 gaatcgcgcg gccatcgacg agccggagag cttcgagaac gggtcttcgg gcgcgagcgg
     4741 ctgcggcccg acggaggcgt gcaaccgtcc gtcggccccg cgtcgggcgc gtgcgacgat
     4801 gcggagcggg gcgccggcgc gcttcgccgc gcgcacgtct tccgcgtcga gcccgcgaat
     4861 accttcgcgc cgcacgtcgc cgagcttgag gcgggcgtcc atcaacacgt tggcgagcgc
     4921 gcagattttc acggccgcgt cccacccgtc aacgtcgttc gacgggtcgg tctcggccag
     4981 tcccaactcc tgcgcgcgcc gcacgccctc gtcaaacgtc gcaccttcct caatcgcttc
     5041 gaggatcacc gagctggtgg cgttcaggag gccgcgcacg gaaaggagcc gcgccgcggg
     5101 gagcgacgcg cgaaagagcg agaagatggg caggcagtcg gcgacggccg actcgaacat
     5161 gaagcgacgg ccgcgcttgc gcgccagagc gtccaactcc tcgaaggcgt ggacgaccgg
     5221 ccccttgttg gccgtgacgg cgtgcgcccc gcgctcaaga gccgcgcgga tgtgggagac
     5281 ggccggctcg cccgcgtgcg tgtcgaggga ggtattctca aagagcactt cggtgcgcgc
     5341 ggcgtcgagc cattcgccca cgtccttcgg ctcactaccg acgcgcgcac gcagctctcc
     5401 cgattccagc tcggcgtcgt cgaagccttc gggcgcgatg agccagccca tgcggcgcgt
     5461 cgccacgccc gtgacgcgcc actcgatgcc gtactggtcg cgcatctcgt cgcgcttctc
     5521 gacgagcaga cgggccaggg cgcggccgac gtttccgaac ccgagcaggc agaggttgta
     5581 agttttcagc gaagacattt gtcccgagcg tcctcttatg tcaggggatg aggaaagagg
     5641 cgcgctccgg gcgcgcgcct ctttccgtgc gtcacatcat cccggccgca acgctatggc
     5701 tgcgggaacg gcgggggcgt gaagttggga acgaacccca gcgccgcggg gaagctggcg
     5761 ggcgtcgggc gcacgccggt cttcacccag ttctcgatgg cggcgacgga cgcgaagagc
     5821 tgttgcggcg tgaagtcaca gtggccgatc tggccgggca cgacgccgag ctggctgccg
     5881 ctcgtgtaga cctgaaacag gcggtcggcg cgccccgccg cctcgaccgt ctcgcggtag
     5941 gccgactcct gcgtgacgac gacgaggccg tcaacgatgg tgtgcagcgt gagcacgggg
     6001 tgcttgatct tgccgctgta gtcggcgttg cgctccacgt agttgcgtga atggttaggc
     6061 gcggagacgt tcgtccgcgc gttcatcgcg gcgagccacg cgtcggcgac ggccgggctg
     6121 acgccgaagc tctggaggta cgccttctcg gcgggcgtca ggctgtaggt gtggttcagg
     6181 ttctgcacga cggggccgcc cgcgcgcctc tctaactcgg cccgtgcctc ggtggcgaag
     6241 aacatgtcgg tcaaaagcca gccggggtag aagaacggcg ggggcggcgg ctggatgccg
     6301 cggccgggat tgccgatgac gaggcggaca aactcgaact tcccgaagtt gagcgggttg
     6361 ccgatctccg ccagaagctt cgggcgcacg tccgtctcga agtctatgtc gtcgcgcgcg
     6421 tcgccgacct cgccccaggt cgagggcgcg ccgaagacgg tgtcgtaggc gagccacagg
     6481 tcggccgagc tgtcccaggt gcgcgtcgcg ccggcgccgc cggcgcacgc cgcaatcgcg
     6541 ccgtcgtaga cgccgccgaa gtgttccatg ctcttgagag tgatgaagga accgagcgag
     6601 aagccccaga tgatcgtgcg gtcgggcttc gtgacgtagg cgcggaaata gttcacgagg
     6661 tttttcatgt cctgcacgcc ctcctcgacg gcccacccgt tgtcgcggta ggccgagccg
     6721 gcgagggcgt agccttgcgc gagcagcgcg gggtcgagcg cggggttcgg ggaggcgtcg
     6781 gccgtgcggt tatccacctc gccggggtgg tcggccttgt cgcggtagcc gtgcgcgaac
     6841 accagcagcg tcccgttcca ggcggccggc accttgatgc ggtagggcgc gccgcctatc
     6901 tcgccctgga ggtcgagcgg cccgacctga tcggccgcgt gggccgagaa cgaaaaggcg
     6961 aagagcagcg acaggaagag gacgaaggtc gggggttttc tgtgcatttt ttgtgttcct
     7021 ccagcaaggg atttcgggca agttcggggc gccggcgacg aagggcgagc aaccagggcg
     7081 agcaacccgc ggcggtaatc cggttttaag aatgctgccg taaggtatgg caggggccgc
     7141 ggcggtgtca agatttttcg gccgcgtcgc gttcgagcgg cgcctcgtac attttgtagt
     7201 gggtctcgct catcccgagc cgctcgtagg tgtgttgagc ggccacgttc tcgcgttcga
     7261 cgtagagccg gaagccgcgc acacgcgggt cggcggcggc gagtcgtttg acgaactcgt
     7321 agagcaccgt gtagacgccg cggcggcgat gctcggcgcg cacgtagacg ctctgcaccc
     7381 accagaagag accgtcgcgc cagtcgctcc actcgtaagt aatcatcaac gagccggcgc
     7441 attcgaccgc gccgtcgccg gggccgcgcg cttccgccat cacatagaag ccgaactgtg
     7501 ggttgtcgta gagagcacgg acgcctgatg cgagcgtcgc tggcgagagt tccagcgact
     7561 cggtctcgcg ggccatcgcg aggttgaact cgacgatgag ggccgcgtcg tcgcgtgtcg
     7621 cgagccgcgc cgtgtattca gtgtcttgca ttcgtcgtcg tcctcacccg cgctcgttga
     7681 tccagacggg tttcgagttg cagccggtgc cgtcgctgcg ccagcgcggg cacgaccaga
     7741 agtgactgcc ggcgctcgtc gggccgcgtt tgaccgtggg cgtcccgcac agcgcgcatt
     7801 ccggtcccgt ctcgggcgcg ggccgcggct cctgaagcgc gccgccctcg tcgaaccagc
     7861 gcacggcctg acacgccggg tagcgcgagc acgcgagcga cgcgttgcgg cccttctcgc
     7921 gcggcagcct cagacgcacc gctccaccgc acgccgcgca cttcgtctcg aagagcgcct
     7981 tgcgttgctt cgcgttcttc ggcgtgtcgc tcaggaagcc gcaagcctct ttgccaagtg
     8041 agcaggcgag gaacttcccg gccgtgctcg tgcgttcgac gaggaagccg gcgcgttctt
     8101 ccgccttgca cttcgggcaa tcgtgcgcgc cttcgggccg cggcgcggcg ctgcgcgcgg
     8161 aggcgggcgc gccctcgacc tgcgagccgg cgatctgcgc gaccagctcc acgactatcc
     8221 ccttgacctc gcccatgaag gtgtcgaaag cgtactcgcc gcgcgtcata cgcgaaagct
     8281 tctgctccca catccccgtc aactcggccg acttgagcag agggtacggg agcatacgga
     8341 tgagcgccgc gcccttctcc gtggcgacga gcgacttctt ctcgcgcctg acgtattcgc
     8401 gcttgagaag cgtctcgatg atgttggcgc gcgtggccgg cgtgcccagg cccgcctcct
     8461 tcatcgcgag acgcagctcc tcgtcctcga ccttccgccc ggcggtctcc atcgcgccga
     8521 ggagcgccga ctccgagtag cgcggcggcg ccttcgtctg cttggcgagc gcctcggcgt
     8581 gcgtcacctc ggccgagtcc ttcgccttga gc
//
LOCUS       DASVES010000495         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_256365, whole genome shotgun sequence.
ACCESSION   DASVES010000495 DASVES010000000
VERSION     DASVES010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256365"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF240_20880"
     CDS             complement(<1..318)
                     /locus_tag="VF240_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177727.1"
                     /translation="MIRRLAFAAIIVLCALSFAYANLQQTQKSILNETKEARMFQGLR
                     TVIYHVDDLQKAKEWYSNMLGIKPYFDEAFYVGFNVGGFELGLDPDMKSISKGNNVIA
                     YWGV"
     gene            609..1148
                     /locus_tag="VF240_20885"
     CDS             609..1148
                     /locus_tag="VF240_20885"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7177728.1"
                     /translation="MGYGWEGEKVRLVPLDKARHMENALAWINDPDVTETTLTGDIPI
                     ARLAEEAWFDRMCAEMSPTPADIVFAVETLAGEHVGSAGLHRIDWRHGSALTGTLIGP
                     REARGQGYGTDAARVRTRYAFEVLGLRLLLSEVIAENAASLRMLERAGYREVGRIPRR
                     YWRRGAYREAVLLAAERGG"
     gene            1336..3318
                     /locus_tag="VF240_20890"
     CDS             1336..3318
                     /locus_tag="VF240_20890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7177729.1"
                     /translation="MRLIATPIATRATAARLALALAVALVAVGFAATPAPAQKKHHES
                     AKSPASARKVRQYTIEQFMDTVRLGGASFSPDEKQILFSSNKTGIFNVYTVPTTGGEA
                     KQLTTSEKESTFSVSYFPTDTRFIYSYDRGGNENSHLYLRELDGTERDLTPGDKTKAN
                     FLGWSHDRKSFFFSTNERDPKYFDTFEMNIADMKPTLVFKNEVGYSLADISNDKRYIA
                     FVKPGESTADSDIFLYDMQKKEMKKITPHTGEISYSPESFDPQSKSLYFTTDEGGEFA
                     YLKRYDLATGKSEVVERANWDISGAYFSRNGKYRVTVLNEDARTVIKIYDTATGKLVP
                     LPKLPGGDITGVNIAPSESRMAFYLNGDRSPSNLYVYDFATKKATRLTNSLNPQIDAA
                     DLVEAENIRYKSFDGMEIPGILYKPHGASAASKAPAIVLVHGGPGGQARRGYKAQVQF
                     LVNHGYAVLDVNNRGSSGYGKTFFTADDRKHGREPLWDVVEAKKYLTATGWVDEKKIG
                     IMGGSYGGYMVLAALAFKPEEFAVGVDIFGVSNWVRTLQSIPPYWEAQRKALYKEIGD
                     PATQLEMLRAASPLFHADKIRRPLIVLQGKNDPRVILPESDEIVEAVKKNGVPVEYVV
                     FDNEGHGFTKKANEIRASQAILEFLDKHLKAGASAN"
BASE COUNT          725 a         1175 c         1018 g          612 t
ORIGIN      
        1 cacgccccag taagcaataa cgttgttgcc ttttgaaata ctcttcatgt ccgggtcaag
       61 ccctaactcg aagccaccaa cattaaagcc gacataaaat gcctcgtcaa aataaggctt
      121 gatacccagc atattggaat accattcttt agccttctgt aaatcatcaa cgtgatagat
      181 aaccgttctt agtccctgaa acattcgcgc ctcctttgtc tcattcagaa tggatttttg
      241 tgtctgctgc aaattggcat aggcaaacga gagagcacag agcacaataa tagctgcgaa
      301 agcaagtctc cgaatcatgt tctttccttt ctgctgtcta aattcaaacc cacgagcaag
      361 ccatctaacg catattaggc gtactaaaag gcgtattgtt ttacgccaat gtgggcgcat
      421 aataatctct gtccatcaat tttactagag tttccgaggc gttccggtgt gttaggcgga
      481 ttcgtcgcgg gcgtgttaga cggccgacat ctcgggcaat gccggagccg tgtttctccc
      541 cccggccttc tcatcccggg cgattgtttg taagcttgtg cgcgcacatc gggggaggag
      601 gattttttgt gggctacgga tgggagggtg agaaggtgcg tctggtgccg ctcgacaagg
      661 cgcggcacat ggagaacgcg ctcgcgtgga ttaacgaccc ggacgtgacc gagacgacgc
      721 tgacgggtga catccccatt gcgcggctcg ccgaggaggc gtggttcgac cggatgtgcg
      781 ctgagatgtc gccgacgccg gcggacatcg tcttcgccgt cgagacgctc gcgggcgagc
      841 acgtcgggtc ggcggggctg caccgcatcg actggcggca cggctcggcc ctcaccggca
      901 cgctcatcgg cccccgcgag gcccgcggcc agggctacgg caccgacgcg gcgcgcgtcc
      961 gcacgcgcta cgccttcgag gtgctgggcc tgcgtctgct gctctcggag gtgatcgccg
     1021 aaaacgccgc ctcgctccgc atgctggaac gcgccggcta ccgggaggtc gggcgcatcc
     1081 cgcgccgcta ctggcgccgc ggggcctacc gcgaagccgt gctgctcgcg gccgagcgcg
     1141 gcggctgaca ggggcgacac aagtaaaaat ctcgcctcac ttgaaactcc cgcgccttcc
     1201 gggacgtttc tatcaaagtc tcgcgaaaga ttaacgccgg acgcgccccg tccggcccgc
     1261 gcaccatgac aacccccggc ggcgactcat tgaagtcgcc cgcacaacac tgacacgccc
     1321 ttaaaggaaa aatctatgag actcatcgca actcccatcg ccacgcgcgc gacggccgcc
     1381 cgtctcgctc tggcactggc cgtggccctt gtcgccgtcg gcttcgcggc cacgcccgcg
     1441 ccggcgcaga agaagcatca cgagtcggcc aagtcgccag cctcggcgcg caaggtccgg
     1501 cagtacacca tcgagcagtt catggacacc gtccgcctcg gcggcgcgtc cttctcgccc
     1561 gacgagaaac aaatcctctt ctccagcaac aagacgggca tcttcaacgt ctacaccgtg
     1621 cccaccacgg gcggcgaggc caagcagttg accacctcgg agaaggagag caccttctcc
     1681 gtctcctact tccccaccga cacgcgcttc atctactcct acgaccgcgg cggcaatgag
     1741 aacagccacc tctacctgcg cgaactcgac ggcaccgagc gcgacctcac gcccggcgac
     1801 aagaccaagg ccaacttcct cggctggtcg cacgaccgca agtcgttctt cttctcgacg
     1861 aacgagcgcg acccgaaata cttcgacacc ttcgagatga acatcgccga catgaagccc
     1921 acgctcgtct tcaaaaacga ggtcggctac agcctcgccg acatctccaa cgacaagcgc
     1981 tacatcgcct tcgtcaagcc gggcgaatcc actgccgatt ccgacatctt cctctacgac
     2041 atgcagaaga aggagatgaa gaagatcacg ccgcacacgg gcgagatcag ctacagcccg
     2101 gagtccttcg acccgcagtc gaagtcgctc tacttcacca cggacgaggg cggcgagttc
     2161 gcctacctca aacgctacga tctcgcgacg ggcaagtcgg aggtggtcga gcgcgccaac
     2221 tgggacatct cgggcgccta cttctcacgc aacggcaagt accgcgtcac cgtccttaac
     2281 gaagacgcac gcacggtcat caagatttac gacaccgcta cggggaagct cgtccccctg
     2341 ccgaagctgc ccggcggcga catcaccggc gtgaacatcg ccccgagcga aagccgcatg
     2401 gccttctact taaacggcga ccgctcgccc tcgaatctct acgtctacga cttcgcgacg
     2461 aagaaggcga cgcgtctcac caacagtctc aacccgcaga tcgacgcggc cgatctcgtc
     2521 gaagccgaga acatccgcta caagtcgttc gacgggatgg aaatccccgg catcctctac
     2581 aagccgcacg gggcgagtgc tgcgagcaag gcgccggcca tcgtcttagt ccacggcggc
     2641 cccggcggtc aggcccggcg cggctacaag gcgcaggtgc agttcctcgt caaccacggc
     2701 tacgcggtct tagacgtgaa caaccgcggc tcctccggtt acggcaagac gttctttacg
     2761 gccgacgacc gcaagcacgg gcgcgagccg ctgtgggacg tggtcgaggc gaagaagtat
     2821 ctgacggcga cgggctgggt cgatgagaag aagatcggca tcatgggcgg ctcctacggc
     2881 ggctacatgg ttctcgcggc gctcgccttc aagcccgaag agttcgccgt cggcgtggac
     2941 atcttcggcg tctcgaactg ggtgcgcacc cttcagtcca tcccgcccta ctgggaggcg
     3001 caacgcaagg cgctctacaa ggagatcggc gaccccgcga cgcagctcga aatgctccgc
     3061 gcggcctcgc cgctcttcca cgccgacaag attcgccggc cgctcatcgt gttgcagggc
     3121 aagaacgacc cgcgcgtcat cctgccggag tcggacgaga tcgtcgaggc cgtgaagaag
     3181 aacggcgtgc ccgtcgagta cgtcgtcttc gacaacgagg gccacggctt caccaagaag
     3241 gccaacgaga ttcgggccag ccaggcgatc ctcgaattcc tcgacaagca cctgaaggcg
     3301 ggcgcgagcg ccaactgacc gcgagccgac attgacgggg ccgctgtcgc ccgcgggaga
     3361 gacgtgaacg gtcgtctctc ccgcgggcga ctttttttgt gtcgccgcgc gtggtctaac
     3421 gtgtcccgcc ggggagactc ggccaaagtt aacaacggtt gagcttagtc gccggcagac
     3481 gcggctgcgc agcgcgcccc tgcccgtcgc aacagtaacg caacttttcc
//
LOCUS       DASVES010000496         9534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1462841, whole genome shotgun sequence.
ACCESSION   DASVES010000496 DASVES010000000
VERSION     DASVES010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462841"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VF240_20895"
     CDS             <1..741
                     /locus_tag="VF240_20895"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX7177730.1"
                     /translation="TPPTLYPTLYLLHGLTGHHTDWLARTKLSDYAAQYRLIIITPEG
                     NDGWYTDSAGVATDRYESYIIKELIPDVERRFRVARAREGRGVAGLSMGGYGALKFGL
                     KYPELFAFAASMSGAVGAASWRTAEDLGRSEFIRQSLVQTFGDGDTPAKRTNDIFRLV
                     RELPEEKTPALPFLYLDCGTEDPLQLLGPNAAFAALLVERKVAHEYRQLPGKHDWLYW
                     DRQVQEVLRLASHKLAAPAPAARGASAK"
     gene            839..2176
                     /locus_tag="VF240_20900"
     CDS             839..2176
                     /locus_tag="VF240_20900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7177731.1"
                     /translation="MRRLFDASAAALLLLLIFFAPHARAQNYEKPPGETQAARVRDLQ
                     AALREARLDGWLFYDFRRSDPLAYRILKLDDKSLTTRRWLYYVPATGEPTRIVHSIER
                     FKLDALPGKKVIYRRWQELHAAVRAALSGRADAPRRAVKRTIAMQYSPMNDIPYVSRV
                     DAGTLEMIRTAGANVVTSADLVQRFEAVWTDEQKRTHDEAANKIRRIIADSFAEIARR
                     TNANEPTNEYDIQQFMLRRYEEEGLTSDNDPPNVSVGPNSANPHYQPDAKTHSPIRRG
                     DFVLFDVWAKLKQPGSIYADQTWVGYVGETVPEEHQKIFRIVAEARDAATNFVRAALR
                     DGKVIGGAQVDDVSRGVVERAGYGEQFTHRTGHSIGEEVHGNGAHIDNLETRDSRRII
                     PRTGFSIEPGIYLEGKFGVRSEINVYASERDIEVTGQPIQTEIVPILKVSSKQ"
     gene            2258..3028
                     /locus_tag="VF240_20905"
     CDS             2258..3028
                     /locus_tag="VF240_20905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7177732.1"
                     /translation="MLNFAIQTAREAGRVLMEKFGRAEINHKGDIDLVTEADLAAERL
                     IIERISGHFPRHAILSEEAGDVRIHGSENSAWKWIVDPLDGTTNYAHGYPVFCVSIGL
                     EHEGRIVAGVVHDPTRDETFAAERGAGATLNGRSVRVSDVDDFNQALVCTGFPYDVRG
                     RDNFARHFTNFIMRAQSLRRDGSAALDLAYVACGRFDAFYEDGLRPWDVAAGTLLVEE
                     AGGRVTHYDGSPFDTYTPPLAASNGLIHDELLGVLQEP"
     gene            3210..4985
                     /locus_tag="VF240_20910"
     CDS             3210..4985
                     /locus_tag="VF240_20910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7177733.1"
                     /translation="MIAPDTVLQNRYVVLEQIGQGGMGAVYVATDQRFGSTVALKETF
                     FDDPALRKAFEREAHLLNRLRHPALPRVSDHFGEGTGQFLVMEFIPGDDLSEMLEKRG
                     GAAFPLEQVLLWADQLLDALDYLHTHEPAVVHRDIKPQNLKLTSRNQIVLLDFGLAKG
                     TAAALSRASMTGSVFGYSRNYAPLEQMQGTGTDPRSDIYSLAATLYHLLTGAVPPDAL
                     TRAMSAVNEQPDPLRPADEMHVQVTRSVAAALSKAMRQSSSQRFQTAEEMRAALRDAA
                     SRRAEEERALATVLTTTPGTAADTTPPDALHDQETQLLGGQTNARADRATRAAEADRA
                     TAHLLGTSAASAPDAAATLLGPGLTAAAGGARPPSAPAAADARADDSVVTRVRPVGST
                     HVVGRRGLSGRTLGAAASVLVLLVAAGAFYAFTRRNDAPQTPTTPEPRQTVATPEPQP
                     SAQTVVEAAPTAPQTAAPSPAPASEAAPAQARQAPAAAKGGKRAEGRNDAVTVIDETK
                     IEEQVRDAERQALTEVGVPEVEIPEHLEGMSPEERERLRNLPRRLRQMMLQQRRIQRQ
                     RQRALEEAERRRRQQQPLPPDAPRP"
     gene            complement(5107..5799)
                     /locus_tag="VF240_20915"
     CDS             complement(5107..5799)
                     /locus_tag="VF240_20915"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7177734.1"
                     /translation="MNDGTSTARDDTQGRAYRGGEEPRPLPEWFLLGLHAPTHLLSRL
                     VWRIRLRGLEHIPAPERGGLVIAANHQTYVDPFWVTIPVMRPLRHLAWSEQFKKPVLG
                     KLLDLLGAWPLQIDRSDPRAYRRSLRWLRAGGALVIFPEGERSEADGLMETFKAGAVR
                     LALEAGVPVLPVTIRGGNRAWPRGQKWPRTGRVEIVYHPVQRLAPRPGEDARQCARRE
                     TERLEAQIKSAL"
     gene            complement(5796..6194)
                     /locus_tag="VF240_20920"
     CDS             complement(5796..6194)
                     /locus_tag="VF240_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177735.1"
                     /translation="MSEEEKKKVRRRVVVPRQLLLVEVERYCRDPQCRARTGVGLTKE
                     EARTYDAFTCARCEQKWDDALTERDIPEWWEELRITSLDARRSPALRPGAEEASEGPV
                     TRMSDAWRARSERGDREADGEGASDGEDSL"
     gene            complement(6290..7054)
                     /locus_tag="VF240_20925"
     CDS             complement(6290..7054)
                     /locus_tag="VF240_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177736.1"
                     /translation="MTYEGKSRRLRERVKLTLPVRVECRETADFRWTEVSRLVDVTPF
                     GAGLALTHQTETGRLLLLTLPMPRQLRSFDHAEDQYKVWALVRNVRPHQSAAASRPDG
                     PRFEVGVAFVGKRAPASYEQDPAQRYDVQSLTPEGNSYALVEWSAPAQEAGAGAARAM
                     ETRLSMPVDVVVEAFDERGVVQEREQTVTENISRHGASVWTTLGLERGRFVRLTSVAQ
                     RVSIAAVIRSRRAGPDGVMRLHLEFVDREWPLEGLE"
     gene            7361..>9534
                     /gene="gyrA"
                     /locus_tag="VF240_20930"
     CDS             7361..>9534
                     /gene="gyrA"
                     /locus_tag="VF240_20930"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7177737.1"
                     /translation="METAEHNYINIEEEMRRSYLDYAMSVIIGRALPDVRDGFKPVHR
                     RVLWAMQELGNQYNKAYKKSARIVGDTIGKYHPHGDSAVYETIVRMAQDFSMRYPLID
                     GQGNFGSIDGDAAAAMRYTEARMAKLTSEILADIEKETVNFNPNYDESLSEPAVLPTR
                     VPTLLLNGSGGIAVGMATNIPPHNLTEVIDATITLVKKPGVKIEELMKIIPGPDFPTG
                     GFIYGREGIKQAYLTGRGIIQMRARAGIDRIGRGQTERDAVIITEIPFQVNKARLIER
                     IAELVNEKKIDGISDLRDESSRDGMRVVVELKRDAIPQIVLNKLYKLTPMQSSFGVIN
                     LAIVSGQPRVLNLKQMLEHFVEFRREVVRRRTEYELRKARARAHILEGLNKAIDALDY
                     IIPLIRNARAVDEARLWLTGNFDGMAQVKQWKGAPSDQTLAGFIQKLQKVIKRLEFSE
                     LQAQAILDLQLRRLSALERQKILDEYEGIIRLMAELEDILGNEASLRRVIIGELEEVR
                     KNFADARRTQIIDEGVELTVEDMIADEDVAITVTNSGYMKRTAVSTYQRQGRGGKGRF
                     GAGTGKSDDFVEHLFIASTHSYLMIFTDDGQVFKLKVHEIPDALPSARGKAVVNLINI
                     PTTRKLVGVIPVREFSEGRYVVMVTRKGVIKKTSLADFQNIRTNGINAVNVDDGDELL
                     DVILTDGTKRIFIATHNGLAIRFEEDDVRSMGRAARGVRGIDL"
BASE COUNT         1727 a         3486 c         2891 g         1430 t
ORIGIN      
        1 acgccgccga cgctctaccc gacgctctac ctgctccacg ggttgacggg ccatcacacc
       61 gactggctcg cgcgcaccaa gctctccgac tacgccgcgc agtaccgtct catcatcatc
      121 acgcccgagg gcaacgacgg ctggtacacc gacagcgcgg gcgtggcgac ggacaggtac
      181 gagtcgtaca tcatcaagga gttgatcccc gacgtggagc ggcgtttccg cgtcgcgcgc
      241 gcgcgcgagg gacgcggcgt cgccggtctc tcgatgggcg gctacggcgc gctgaagttt
      301 ggcttgaagt acccggagct gttcgccttc gccgcttcga tgagcggcgc ggtgggcgcc
      361 gcctcgtggc gcacggcgga agacctcggc cgctcggagt tcatccgcca gtcgctcgtc
      421 cagaccttcg gcgacggcga cacgccggcc aagcgcacga acgacatctt ccgactcgtc
      481 cgcgagctgc ccgaagagaa gacgccggcg ctccccttcc tctatctcga ctgcggcacc
      541 gaagacccgc tccaactgct cggcccgaac gccgccttcg ccgccctgct cgtcgagcgc
      601 aaggtcgcac acgagtatcg ccaactcccc ggcaagcacg actggctcta ctgggacagg
      661 caggtgcagg aagttctgcg cctcgcctct cacaagctcg ccgcgcccgc cccggcggcg
      721 cgcggcgcat cagcaaagta ggacaggaat tcgcggcctg tcttgagcgc gcgagcgcga
      781 aaacaagcat tcgccgaagg ttgtacgagt gaggccccgc ggggtaaaac taaacgccat
      841 gagaagatta ttcgatgcgt cggccgccgc cctcctcctc ctgctcatct ttttcgcccc
      901 ccacgcgcgc gcgcagaact acgagaagcc gccgggcgag acgcaggccg cgcgcgtccg
      961 cgaccttcag gccgccctgc gcgaagcccg cctcgacggc tggctcttct acgacttccg
     1021 ccgcagcgat cccctcgcct atcgcatcct gaagctcgac gacaagagtc tgaccacgcg
     1081 tcgctggctc tactacgtgc ccgccacggg cgagccgaca cgcatcgtcc actccatcga
     1141 acgcttcaag ttagacgcct tgcccggcaa gaaagtcatc taccgccgct ggcaggaact
     1201 ccacgccgcc gtgcgcgccg ccctttccgg ccgcgccgat gctccgcgcc gcgcggtgaa
     1261 gcgaaccatc gccatgcaat actcgccgat gaacgacatc ccctacgtct cgcgcgtgga
     1321 cgccggaacg ctggaaatga ttcgcacggc cggcgcgaac gtcgtcacca gcgccgacct
     1381 cgtccaacgc ttcgaagccg tctggactga cgagcagaaa cgcacgcacg acgaagccgc
     1441 caacaagatt cgacgcatca tcgccgactc cttcgccgag atcgcgcgcc gcacgaacgc
     1501 caacgagccg acgaacgagt acgacatcca gcagttcatg ctgcgtcgct acgaggagga
     1561 ggggctgacc tccgacaacg acccgcccaa cgtctcggtc ggccccaaca gcgccaaccc
     1621 acactaccag cccgacgcca aaacccactc gcccatccgc cgcggcgact tcgtcctctt
     1681 cgacgtgtgg gccaagctca aacaacccgg ctccatctac gccgaccaga catgggtggg
     1741 ctacgtcggc gagacggtgc ccgaagagca ccagaagatt ttccgcatcg tcgccgaggc
     1801 gcgcgacgcc gccaccaact tcgtccgcgc ggccctgcgc gacggcaaag tgatcggcgg
     1861 cgcgcaggtc gatgacgtgt cgcgcggtgt cgtggagcgc gccggctacg gcgagcagtt
     1921 tacgcaccgc accggccact ccatcggcga ggaggtacac ggcaacggcg cgcacatcga
     1981 caatctggag acccgcgact cgcgccgcat catcccccgc accggcttct ccatcgaacc
     2041 cggcatctac ctcgaaggca aattcggcgt ccgctccgag atcaacgtct acgcctccga
     2101 gcgcgacatc gaggtgacgg gacagcccat ccaaaccgag atcgtgccga tactgaaagt
     2161 gagcagtaag cagtgagcgg taagcagtag acgctccgcg ccgtcgcccc gcgcacaggt
     2221 ttgacaactg ctcactgctt actgctcact gcttactatg ttgaattttg ccatccagac
     2281 cgcccgcgag gcgggccgcg tgctcatgga gaagttcggg cgcgctgaaa tcaaccacaa
     2341 gggcgacatc gacctcgtca ccgaggccga cctcgccgcc gagcgcctca tcatcgagcg
     2401 catcagcggc cacttcccgc gccacgccat tctctcggag gaggcgggcg acgtgcgtat
     2461 ccacggctcc gagaactccg cgtggaagtg gatcgtcgat ccgctcgacg ggacgaccaa
     2521 ctacgcgcat ggctacccgg tcttctgcgt ctcgataggt ctggagcacg aggggcgcat
     2581 cgtcgccggc gtcgtccatg acccgacgcg cgacgagacc ttcgccgccg agcgcggtgc
     2641 gggcgcgacc ttgaacggcc gctccgtgcg cgtctccgac gtggacgact tcaatcaggc
     2701 gctcgtctgc accggcttcc cctacgacgt gcggggccgc gacaacttcg cgcgccactt
     2761 caccaacttc atcatgcgcg cgcagtcttt gcgtcgcgac ggctccgccg ccctcgacct
     2821 cgcctacgtc gcctgcggcc gcttcgacgc cttctatgaa gacggcctcc gcccgtggga
     2881 cgtggccgcc ggcacgctcc tcgtcgaaga ggccggcggc cgcgtcacgc actacgacgg
     2941 ctcccccttc gacacctaca ccccgccgct cgccgccagc aacggcctca tccacgacga
     3001 actgctcggc gtgctccaag agccgtgaat cgtcaatcgt caatcgtcaa tcgtggttga
     3061 ggggggcgtg gcttgagtgt taaagcgtgc gccggattag cgtcaatcaa tcgatttacg
     3121 atttaccatt tacgattcac gatagattgt tcgagtcgcg cggcgcgggt tatgatgagc
     3181 gccgtgaacg tcgaagaggt gaacgctgaa tgatcgcccc tgacaccgtc ttgcagaacc
     3241 gctacgtcgt gctcgaacag atcgggcagg gcgggatggg cgccgtctac gtcgccaccg
     3301 accagcgctt cggctcgacg gtcgcgctca aggagacttt cttcgacgac ccggcgctac
     3361 gcaaggcatt cgagcgcgag gcgcacctcc tcaaccgcct gcgtcacccg gccctgccgc
     3421 gtgtcagcga ccactttggc gagggtacgg ggcaattcct cgtcatggag ttcatccccg
     3481 gcgacgacct ctcggagatg ttggagaagc gaggcggcgc cgccttcccg ctcgaacaag
     3541 tgctcctctg ggccgaccag ctcctcgacg cgctcgacta cctgcacacg cacgagccgg
     3601 cggtcgtcca ccgcgacatc aagccgcaga acctcaagct cacctcgcgc aaccagatcg
     3661 tcctgctcga cttcggcctc gccaagggca cggccgccgc gctgtcgcgc gccagcatga
     3721 cgggcagcgt cttcggctac tcgcgcaact acgccccgct cgaacagatg caggggacgg
     3781 gaaccgaccc gcgcagcgac atctattcgc tcgccgccac gctctaccac ctgctcaccg
     3841 gcgccgtccc gcccgacgcg ctcacgcgcg ccatgtcggc cgtcaacgaa cagcccgacc
     3901 cgctgcgccc cgccgacgaa atgcacgtgc aggtgacgcg ctcggtcgcc gccgcactct
     3961 cgaaggccat gcggcagagt tcgtcgcaac gcttccagac ggccgaagag atgcgcgccg
     4021 ccctgcgcga tgccgccagc cgccgcgccg aagaggagcg cgcgctggcg accgtgctca
     4081 cgacgacacc cggcacagcc gccgacacca cgccccccga cgccctccac gatcaggaga
     4141 cgcagttgct cggcgggcaa acgaacgcgc gcgccgaccg ggccacacgc gccgccgaag
     4201 ccgaccgcgc taccgcccat ctcctcggta cgagcgccgc ctccgccccc gacgccgcgg
     4261 cgaccctgct cggccccgga ctgaccgccg cagccggcgg cgcacgcccc ccgtccgcgc
     4321 cggcggcagc ggatgcgcgc gcggatgatt ccgtcgtgac acgcgtccgc cccgtcggca
     4381 gcacgcacgt cgtcggcaga cgtgggcttt caggtcgcac gctcggcgcg gcggccagcg
     4441 tcctcgtcct gctcgtcgcg gccggcgcct tctacgcctt cacgcgccgc aacgacgcgc
     4501 cgcaaacgcc cacgacaccg gagccgcggc agactgtcgc gacaccggag ccgcagccct
     4561 cggcgcagac cgtcgtcgag gccgcgccga ccgcgccgca gacggccgcg ccgagtcccg
     4621 cgcccgcctc tgaggccgcg ccggcgcagg ccagacaagc gccggcggca gccaaaggag
     4681 gcaagcgcgc ggagggccgc aacgacgccg tcaccgtgat tgacgagacg aaaatcgagg
     4741 agcaggtgag agacgccgag cggcaagccc tcactgaagt gggagtgccg gaggtcgaga
     4801 ttcccgaaca tctggagggg atgagtcccg aggagcgcga gcgtctgcgg aacctgccgc
     4861 gccggctcag gcagatgatg ttgcagcagc ggcggataca gcgtcagcgc cagcgcgcgc
     4921 tcgaagaggc cgagcggcgg cggcggcaac aacagccgtt gccgccggat gcccctcggc
     4981 cataagtcga ttgacgtagg acaggcattc ctgcctgtca cgtcttgcct ggagttcctg
     5041 cctaacatgc aagacgtgac aggcaggaat gcctgtccta catggccgcc cgtttgaacc
     5101 gtccacttac aacgcggatt tgatctgcgc ttcgagccgc tcggtctcgc gccgcgcgca
     5161 ctgccgcgcg tcctcgccgg gacgcggcgc gagtctctgc acgggatgat agacgatctc
     5221 gacgcggccc gtgcgcggcc acttctgccc gcgcggccaa gcgcggttcc cgccccgaat
     5281 cgtcaccggc aggacgggca cgcccgcttc gagcgccagc cgcacggcgc ccgccttgaa
     5341 cgtctccatc agcccgtccg cctctgagcg ttcgccctcc gggaagatga cgagggcgcc
     5401 gcccgcgcgt agccagcgca gcgagcgccg gtaggcgcgc gggtcgctgc ggtcgatttg
     5461 cagcggccac gcgcccagca agtccagaag tttacccagc actggcttct tgaactgctc
     5521 gctccacgcg aggtggcgca gggggcgcat gacggggatc gtgacccaga acggatcgac
     5581 gtaggtctgg tgattggcgg cgatgacgag gccgccgcgc tcgggcgcgg ggatgtgttc
     5641 aaggccgcgc aggcggatgc gccagacgag gcgcgagagc aggtgcgtcg gcgcgtgcag
     5701 acccaacagg aaccactcgg gcagcgggcg cggctcctcg ccgcccctgt aggccctccc
     5761 ctgtgtgtcg tcgcgcgccg tgctcgtgcc gtcgttcaaa gcgaatcctc tccgtcggac
     5821 gcaccctctc cgtccgcctc ccggtcgccc cgctcagaac gcgcccgcca cgcgtcgctc
     5881 atgcgcgtca ccggcccctc cgacgcctcc tccgcccccg gcctcagcgc cggcgagcgc
     5941 cgtgcgtcga gcgaagtgat ccgcaactcc tcccaccact cggggatgtc gcgctccgtg
     6001 agcgcgtcgt cccacttctg ctcacagcgc gcgcacgtga aggcgtcgta ggtgcgcgcc
     6061 tcctccttcg tcagccccac gcccgtgcgc gcacggcact gcgggtcccg gcagtagcgc
     6121 tccacctcga ccagcagcaa ctgccgcggc acgaccaccc gccgcctcac cttctttttc
     6181 tcttcttcag acataatcga aaacaagtga tgagtgatga gtgataagtg ataagaaaag
     6241 tcagcttgct ttctcttatc acttatcact tatcactcat cactcatcac tactccaacc
     6301 cttccagcgg ccactcccgg tcaacgaatt cgaggtggag gcgcatgacg ccgtcggggc
     6361 cggcgcggcg ggagcggatg acggccgcta tcgacacgcg ctgcgcgacg ctcgtcagcc
     6421 gcacgaagcg cccacgctcc agcccgagcg tcgtccagac ggaggcgccg tggcggctga
     6481 tgttctccgt cacggtctgc tcgcgctcct gcacgacacc gcgctcgtcg aacgcctcga
     6541 cgacgacatc gacgggcatc gaaagcctcg tctccatcgc gcgcgccgcg cccgcgcccg
     6601 cctcctgcgc gggcgcgctc cactcgacca gcgcgtagga gttgccctcc ggcgtgagcg
     6661 attgaacgtc gtagcgctgc gccgggtcct gctcgtaact cgcgggcgct cgcttgccga
     6721 cgaaggccac gcccacctcg aagcgcggcc cgtccggccg cgaggcggcc gcgctttggt
     6781 gcgggcgcac gttgcgcacc agcgcccaga ccttgtactg atcctcggcg tggtcgaagc
     6841 tacggagctg gcgcggcatc ggcagcgtga gcagcagcag ccgccccgtc tcagtctggt
     6901 gcgtgagcgc aaggcccgcg ccaaacggcg tcacgtccac gaggcgcgac acctccgtcc
     6961 agcggaagtc ggccgtctcc cggcactcga cgcgaacggg cagcgtgagc ttcacgcgct
     7021 cgcgcagacg ccgcgatttg ccttcgtatg tcatccctcg ccgatgcctc gggccgggcc
     7081 tttcaggagc aaaatgcgcc actcccggag cggccctcgg ggcacccccg ccggtgcctg
     7141 aaaaccttga ttttacagga ctttcggccc aactcggggt ttgccaaatc cgccttttcg
     7201 ggctacaata tctcgtactt tcgtccggcc tacgacagtc ccgaaaagta ccttttgcag
     7261 tgcgttttat cttaaccaaa gaacccggcg ctgccggccc cttcagccgg ccggagtgtg
     7321 tcaagcgaag cgccggagat tgtaccggaa aggtagattg atggaaacag cagagcacaa
     7381 ttacatcaac atcgaagagg agatgcggcg ctcgtacctc gactacgcga tgagcgtcat
     7441 catcggccgc gccctgcccg acgtgcgcga cggcttcaag cccgtccacc ggcgcgtcct
     7501 ctgggcgatg caggaactcg gcaaccagta caacaaggct tacaagaaga gcgcccgcat
     7561 cgtcggcgac actatcggca agtatcaccc gcacggcgac tcggccgtct acgagaccat
     7621 cgtccgcatg gcgcaggact tctccatgcg ctacccgctc atcgacggtc agggcaactt
     7681 cggctccatc gacggcgacg cggccgccgc catgcgctac accgaagcgc gcatggccaa
     7741 gctcacttcg gaaatcctcg ccgacatcga gaaggagacc gtcaacttca acccgaacta
     7801 cgacgagagt ttgagcgagc cggccgtgct gccgacgagg gttccgaccc tgctcttaaa
     7861 cggctcgggc ggcatcgccg tcggcatggc gaccaacatc ccgccgcaca acctcaccga
     7921 ggtcatcgac gccaccatca cgctcgtcaa gaagcccggc gtcaagatcg aagagctgat
     7981 gaagatcatc cccggccccg acttccccac gggcggcttc atctacggcc gcgagggcat
     8041 caagcaggcg tatctgacgg ggcgcggcat catccagatg cgcgcccgcg ccggcatcga
     8101 ccgcatcggg cggggccaga ccgagcgcga cgccgtcatc atcaccgaga ttccttttca
     8161 ggtgaacaag gcgcgcctca tcgagcgcat cgccgaactc gtcaacgaga agaagatcga
     8221 cggcatctcc gatttgcgcg acgagtcgtc gcgcgacggc atgcgcgtcg tcgtcgaact
     8281 caagcgcgac gccatcccgc agatcgtcct caacaagctc tacaagctca ccccgatgca
     8341 gtcctctttc ggcgtcatca acctcgccat cgtctcgggg cagccgcgcg tgctcaacct
     8401 caagcagatg ctcgaacact tcgtcgagtt tcgccgcgag gtggtgcgcc gccgcaccga
     8461 gtacgagttg cgaaaggcgc gcgcgcgcgc acacatcctc gaaggactca acaaggccat
     8521 cgacgcgctc gactacatca tcccgctcat ccgcaacgcc cgcgccgtgg acgaagcgcg
     8581 gctgtggctg accggcaact tcgacggcat ggcgcaggtc aaacagtgga agggcgcgcc
     8641 ctccgaccag acgctcgccg gctttatcca gaaactccag aaggtcatca agcgtctgga
     8701 gttctccgag ctacaggcgc aggccatcct cgacctccag ttgcgccgtc tgtcggcgct
     8761 cgaacgccag aagattctcg acgagtacga gggcatcatc cgcctcatgg ccgagcttga
     8821 agacatcctc ggcaacgagg ccagcctcag gcgcgtcatc atcggcgaac tcgaagaggt
     8881 gcggaagaac ttcgccgacg cccgccgcac gcagatcatc gacgagggcg tggaactcac
     8941 ggtcgaggac atgatcgccg acgaggacgt ggccatcacg gtgacgaatt cgggttacat
     9001 gaagcgcacg gccgtctcga cttatcagcg gcaggggcgc ggcggcaagg gccgcttcgg
     9061 cgcggggacg ggcaagagcg acgacttcgt cgaacacctc ttcatcgcct cgacgcactc
     9121 ctacctgatg atcttcaccg acgacgggca ggtcttcaag ctcaaggtac acgagatacc
     9181 ggacgcgctc ccctcggcgc gcggcaaggc cgtcgtcaac ctcatcaaca tccccacgac
     9241 ccgaaaactc gtgggcgtca tccccgtgcg cgagttctcc gagggccgct acgtcgtcat
     9301 ggtcacgcga aaaggggtca tcaagaagac gagtctggcc gacttccaga acatccgcac
     9361 gaacggcatc aacgccgtca acgtggacga cggcgacgag ctgctcgacg tgattctcac
     9421 cgacggcaca aagcgcatct tcatcgccac gcacaacggc ctcgccatcc gcttcgagga
     9481 ggacgacgtg cggtcgatgg ggcgcgcggc gcgcggcgtg cgcggcatcg acct
//
LOCUS       DASVES010000497         7381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_398492, whole genome shotgun sequence.
ACCESSION   DASVES010000497 DASVES010000000
VERSION     DASVES010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398492"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /gene="cdaA"
                     /locus_tag="VF240_20935"
     CDS             <1..666
                     /gene="cdaA"
                     /locus_tag="VF240_20935"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX7177738.1"
                     /translation="LESILRLSPFVVSVAIVVLFQNEIRQALVQFGNRFRMPFLIRHK
                     GQFGEGVYDEIVLAATTLASTKTGALIVIERGVGLKNVTDGGLKMDAELSYDLLVTIF
                     NPATPLHDGAVVIRRHRVAAAACFLPLTLNPRLSKDLGTRHRAAIGITEDTDAVAVVV
                     SEETGLISFVQAGQIKRALDATRLRAHIFSALESPARRGGGRGQPDAETDLEVRGRRS
                     EVS"
     gene            888..1832
                     /locus_tag="VF240_20940"
     CDS             888..1832
                     /locus_tag="VF240_20940"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX7177739.1"
                     /translation="MSADDAGRVVRRWLREFLLEDWGLKLLAFVITLILWFAVTGQRA
                     PESRQLRDIALEYQLPAELEISNEPLDKVDITLEGSKDKLDEINARDLVARIDIGQLR
                     PGERILRLTRENVSMNLPEGVRVIRIEPPSAALKLERSVERELEVEARLEGQPAEGYE
                     VRGVQITPARVKVRGPESRVGALDKAFTETIPLDGQRETLNAPQVAVDVSDRSVVALD
                     AAVAVRVEIVETQAERRIAGVAVRAATAGAEVRPQTATVVITGPRSVVERLRPEDLTV
                     VVEQAPDGSLRPRLAPAPGLEGRLTLVSVTPADFTIQK"
     gene            1923..3398
                     /locus_tag="VF240_20945"
     CDS             1923..3398
                     /locus_tag="VF240_20945"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7177740.1"
                     /translation="MKKLFGTDGMRGEAGKFPLDAETMTAAGRSLARRLASASPGRAP
                     RIVTGRDTRESGAWIEDAFARGARAGGAGCESAGVVTTPGVAYLARTLPADAGVVVSA
                     SHNPYQDNGIKIFSPTGRKLDDATERLIEADIHAARAGSGPPSDEASALAQDETTSTS
                     SSATSLSNTPRDDKDGADIEANDAHAARLRRRYLNFLVEEVAAGLRLDGLKIVIDCAN
                     GAASRLAPELFRRLGAQVVAFGDAPDGRNINRDCGSLHLEGLCLKVVEESASFGAAFD
                     GDADRALFADARGRVVDGDATLWVLANRMSERGELAGGRVVATVMSNIGLEIALRARS
                     LELLRTDVGDKYVLEELLRTGAALGGEQSGHVIFPSVSLAGDGMITTVHMLRALRETG
                     RELHELTEGFVQYPQVLVNVRVREKLPFQEVAEIASAARGVEKELGAGGRLLLRYSGT
                     EPLARVMIEGESQADIERLAGGLAAVIGRSLGGNEASGEGR"
     gene            3842..5359
                     /locus_tag="VF240_20950"
     CDS             3842..5359
                     /locus_tag="VF240_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177741.1"
                     /translation="MDLGSYRSEYAAYCSALQRERHEHHAGRAPEPHLAPLRDRYADL
                     WTRETIDELRRKLDDTPAHFETERAGLRALVHAACLNHAEASADEVAAELARCEAAAC
                     VEWGGEKIGAAETVELIAREADATRRRELAARWFDALRPCDDLRAARLYALAECVRAL
                     GFDGPRSLYEDASGVSLHGLASAADSFLERTAPTYMSHLARSTARENAFAAVGSLSHA
                     DELFFGRAKRLDALLTPRGERAAYAELMAGLGVRVEQQKNLIVEDGERGASTACFGVR
                     PPDEVYLVAAGDAHAAWKSYSDFFFEAGRAQHFAWSSPETAARRPEFIHAADAAARTA
                     AGFLFKSLFYDATWGVECLRLRPVEARELARSAALLELLDTRRDCAALRYALAVDEAG
                     GVLSEQLAEAYLSLYGEATGFARDPATRLLDTAGALDAAGRLRARLLAASLREHLRSR
                     YGHRWYASRAAGGELTDIWNTALLHPAEQLARLAWGGTLEFDLLTEELKATMEGD"
     gene            5388..6548
                     /gene="aroB"
                     /locus_tag="VF240_20955"
     CDS             5388..6548
                     /gene="aroB"
                     /locus_tag="VF240_20955"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7177742.1"
                     /translation="MTAKALRRVRVRLGGGAEYSIEIGAGALDHLGEAARRVLHAKAR
                     RVALVSNRLVFDLYGARAEESLRAAGFTVAHWLMGDGERHKTLRTAERALEFLSAAGI
                     ERSDAVVALGGGVVGDLAGFAAALHLRGVAFLQAPTTLLAQIDSSVGGKTGVNTHAGK
                     NLVGVFHQPRAVVVDTETLRTLPARELTAGWCEAVKQGAAGDLKLFRRTVKFLAEESE
                     EARSREDELIELIAAHCEFKARIVAGDEREDLDRADAGSRRILNFGHTVGHALEAVTG
                     FRRFRHGEAVGYGMLVAGEISKKLGMLAASELECLREAVRLAGRLPRAGDISEERIRH
                     ALLRDKKSVNGRVLWVLLEGLGRAALVDGSEVSARVLRDALGATLATRSAKS"
     gene            6564..6968
                     /locus_tag="VF240_20960"
     CDS             6564..6968
                     /locus_tag="VF240_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177743.1"
                     /translation="MKHQADTRRRGFADERGAGRLSFYLVVLLLAAAGYVAYQVVPVL
                     YRASTYKVYMQDAVSKGAALGKDPEWVKTQLKTAGVEEYGLPPGALVETHVAEGRINA
                     RVRYTRPIDLTAYVYEYNFDHAVKSDKFLNTQ"
     gene            complement(7068..>7381)
                     /locus_tag="VF240_20965"
     CDS             complement(7068..>7381)
                     /locus_tag="VF240_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177744.1"
                     /translation="SVGRVIESMTRHRGDLQAALRETNFDADFHTGRRRTYDEVLPWE
                     IVDAGLGREFMEREHERAQSAKSTAPCPSVDTCTRCGVCPTTWLAAAPPGLVQLTGAA
                     A"
BASE COUNT         1231 a         2482 c         2460 g         1208 t
ORIGIN      
        1 ctcgaatcca tcctgcgcct gtcgccgttc gtcgtctcgg tcgccatcgt cgtcctcttc
       61 cagaacgaga tacggcaggc gctcgtccag ttcggcaacc gcttccgcat gcccttcctc
      121 atccgccaca agggtcagtt cggcgagggc gtctacgacg agatagtgct ggcggcgacg
      181 acgctcgcct cgacgaagac gggcgcgctc atcgtcatcg agcgcggcgt cgggttgaag
      241 aacgtcaccg atggcggcct caagatggac gccgaactca gttacgatct gctcgtcacg
      301 atcttcaatc cggccacgcc cctgcacgac ggcgcggtcg tcatccgccg tcaccgcgtc
      361 gcggcggcag cctgtttttt gccgctcact ttgaacccgc gtctctcgaa agacctcggc
      421 acgcgccacc gcgccgccat cggcatcacc gaagacaccg acgccgtggc cgtcgtcgtc
      481 tccgaggaga cgggcctcat ctcgttcgta caggccggac agatcaagcg cgccctcgac
      541 gccacgcgcc tgcgcgcaca catcttcagc gccctcgaat cccccgcacg ccgcggcggc
      601 ggccggggac agcccgacgc cgagaccgac ctggaggtca ggggccggag gtcggaagtg
      661 agttaagaga gctgttagct atcagctgtt agcttttagc tctcaagctt gtcagtggtc
      721 agtggtcagt tgtccgttgt ttgcttctgt ccactctacg tctgcgacaa acgattcaat
      781 atcgggaatg aaaaccactg accactgatc actgaccagg aacaaagaac agctcttagc
      841 taacagctaa tagctaaccg cttcacatgg cctttcgtaa cgcagagatg agtgcggacg
      901 acgccgggcg cgtggtgcgt cgctggctgc gcgagttcct gctcgaagac tggggtctga
      961 aactgctcgc gttcgtcatc acgctcatcc tctggttcgc cgtaacgggg cagcgcgcgc
     1021 ccgagtcgcg gcagttgcgc gacatcgcgc tcgaatacca gctcccggct gaactggaga
     1081 ttagcaacga gccgctcgac aaggtggaca tcacgctcga aggcagcaag gacaagctcg
     1141 acgaaatcaa cgcccgtgat ctcgtcgccc gtatcgacat cggccagctc cgccccggcg
     1201 agcgcatcct gcgcttgacg cgggaaaatg tctcgatgaa tctgcccgag ggcgtccgcg
     1261 tcatccgcat tgagccgccg agcgcggcgc tcaagttgga gcgcagcgtc gagcgcgagc
     1321 tggaagtcga ggcgcggctc gaaggccagc cggccgaagg ctacgaggtg cgcggcgtcc
     1381 agatcacgcc ggcgcgcgtt aaggtgcgcg gccccgagag ccgcgtcggc gcgctcgata
     1441 aggcgttcac cgagaccatc ccgctcgacg gtcagcgcga gaccctgaac gcgccgcagg
     1501 tcgccgtcga tgtctccgac cgcagcgtgg tcgcgctcga cgccgcggtg gccgtgcgtg
     1561 tcgagatcgt cgagacgcag gccgagcggc gcatcgcggg cgtcgccgtg cgcgccgcaa
     1621 cggccggcgc ggaggtcagg ccgcagaccg cgacggtcgt catcacgggg ccgcgctcgg
     1681 tcgtcgagcg gctgcgcccc gaagacctca ccgtcgtcgt ggagcaagcg ccggacggct
     1741 cgctccggcc ccggcttgcg cccgcgcccg gactcgaagg gcgcctcact ttggtcagcg
     1801 tgacgcctgc tgatttcacc atccagaaat gacgttcaag ttccgacttt aaagttcaat
     1861 ggctaaggat gtcacccgcc ctcgaacttt gaactcggaa ctttgaaatt ttgaacttaa
     1921 agatgaagaa acttttcggt acggacggga tgcgcggcga ggccggcaag ttcccgctcg
     1981 acgcggagac catgaccgcc gccgggcgct cgctcgcccg gcgccttgcg tcggcgtcgc
     2041 cgggacgcgc gccgcgcatc gtcacggggc gcgacacgcg cgagtcgggc gcatggatcg
     2101 aagatgcttt cgcgcgcggc gcacgcgctg gcggagccgg gtgcgagtcg gccggcgtcg
     2161 tcaccacgcc gggggtcgcc tacctcgcgc gcactctccc ggcggatgcc ggcgtcgtcg
     2221 tcagcgcctc gcacaatccg tatcaggaca acggcatcaa gattttctcc cccaccggcc
     2281 gcaagctcga cgacgccacc gagcgcctca tcgaggccga catccacgcc gcccgcgccg
     2341 gcagcgggcc gccgagtgac gaagcctcgg cgctcgcgca ggacgagacg acatcgacat
     2401 cttcatcagc aacctcttta tcaaacaccc cccgcgacga taaggacggc gcggacatcg
     2461 aggcgaacga cgcgcacgcg gcccgactgc gtcggcgcta cctgaacttc ctcgtcgaag
     2521 aggtcgccgc cgggctgcgg ctcgacggcc tgaagatcgt catcgactgc gccaacggcg
     2581 ccgcgtcccg actcgcaccc gaactcttcc gccgcctcgg cgcgcaggtc gtcgcctttg
     2641 gcgacgcgcc cgacggacgc aacatcaacc gcgactgtgg ctcgctccac ctcgaaggtc
     2701 tctgcctaaa ggtcgtcgaa gaaagcgcct cgttcggcgc ggccttcgac ggcgacgccg
     2761 accgcgccct cttcgccgac gcgcgcggcc gggttgtgga cggcgacgcg acgctctggg
     2821 tgttggccaa ccggatgtcg gagcgcggcg agcttgcggg tgggcgcgtc gtcgccaccg
     2881 tgatgagcaa catcgggctg gagatcgcgc tccgcgctcg ctctctggaa ttgctacgca
     2941 ccgacgtggg cgacaagtac gtgctcgaag agctgctccg cacgggcgcg gcgctcggcg
     3001 gcgagcaatc cggccacgtc atcttcccct cggtgagcct cgcgggcgac ggcatgatta
     3061 cgaccgtcca catgctccgc gcactgcgcg agaccggccg cgagctgcat gagctgaccg
     3121 aggggttcgt gcaatacccg caggtgctcg tcaacgtgcg cgtgcgtgaa aagctgccgt
     3181 ttcaagaggt cgcggagatc gccagcgccg cccgcggggt ggagaaagag ctaggcgcgg
     3241 gagggcggct tctgttgcgc tactcaggca cggagccgct ggcgcgcgtg atgatcgagg
     3301 gtgagagtca ggcggatatc gagcgcctcg cagggggtct cgcggccgtc atcggtcgga
     3361 gcctgggggg caacgaggcg agcggtgagg ggcggtaatt tttacgcagc cgtcgatgta
     3421 actcttacac ttcaccttcg gccgcgtgca aaacctttgc gagtcgggtg gcgcgggttc
     3481 tgtcgattat ggcgcgaaat cctgcactct gagtgataaa tcagaaataa ctacttgcgc
     3541 ggcacgaacg ttgcgatgta tattgtgggg caagagagac ggttggtgaa tcctccgtct
     3601 cagtggttgc tggggtgagg acagtgacca caaattggaa tgggcacggc attccccaag
     3661 tttgccgtgc ccgttcttta tttgccctgg acacacccgc caccgaaccg cttccgcgct
     3721 acgcccgaac gctcaagtcc gaccctccgg catgctacaa tccgcccgtc ctctcggttc
     3781 aaacacatcg acagttttcc gtccgacccg gccgcgacgg gccgcgcgct ttcgttcaag
     3841 tatggacctg ggcagctatc gcagtgagta cgccgcctac tgctccgcgc tccagcgcga
     3901 gcgccacgag catcacgccg ggcgcgcgcc ggagccgcat ctcgcaccct tgagggatcg
     3961 ttacgcagac ctctggacgc gcgagaccat cgacgaactg cgccgcaagc tcgacgacac
     4021 gccggcccac ttcgagaccg agcgcgcggg actgcgcgca ctcgtccacg cggcctgcct
     4081 gaaccacgcc gaggcgagcg ccgatgaggt cgcggccgaa ctggcccgct gtgaggccgc
     4141 ggcgtgcgtc gagtggggcg gcgaaaagat aggcgctgcc gagacggtag aacttatcgc
     4201 gcgcgaggcc gacgcgacgc gccgacgcga actggccgca cgctggttcg acgccctgcg
     4261 cccctgcgat gacctccgcg ccgcgcgcct ctacgcgctg gctgagtgcg ttcgcgcact
     4321 cggctttgac ggcccgcgct cgctttacga agacgcgtcc ggtgtgagcc tgcacggact
     4381 cgcctccgca gccgactcct tccttgagcg cacagcgccg acctacatgt cgcacctcgc
     4441 acgcagcacg gcgcgcgaga atgcgttcgc cgctgtcggc tccctatcac acgccgacga
     4501 gttattcttc gggcgcgcga agcgtttgga cgcgctgctc acgccgcgcg gcgagcgcgc
     4561 cgcctacgcc gagttgatgg ccgggctggg cgtgcgcgtg gagcagcaga agaacctcat
     4621 cgtcgaagat ggagagcggg gcgcgagcac ggcgtgcttc ggcgtccggc cgccggacga
     4681 agtttatctc gtcgccgcgg gcgacgctca cgccgcgtgg aaatcttact cggacttttt
     4741 cttcgaggcg ggccgcgcgc agcacttcgc gtggtcttcg ccggagacgg cggcgcgtcg
     4801 ccccgagttc atccacgccg cggacgccgc cgcgcgcacc gccgccggct ttctcttcaa
     4861 gagtttgttt tacgacgcca cgtggggcgt cgagtgcctg cgcctgcgcc ccgtcgaggc
     4921 tcgcgaactc gcgcgctcgg ccgctctgct ggagctgctg gacacgcggc gcgactgcgc
     4981 ggcgcttcgc tacgcgctcg cggtggacga ggcgggcggc gtgctgtccg aacagcttgc
     5041 ggaggcttac ttgagtctct acggcgaggc cacggggttc gcgcgcgacc ccgcgacgcg
     5101 tctgctcgac accgcgggcg cgctcgacgc ggcgggccgc ctgcgcgccc gtctcctcgc
     5161 cgcctccctg cgcgagcacc tgcgctcgcg ttacggtcac cgctggtacg cctcgcgcgc
     5221 ggcgggcggc gagctgaccg acatctggaa tacggccctg ctccaccccg ccgaacaact
     5281 agcgcggctc gcgtggggcg gcacgctcga attcgatctc ctgacggaag aattgaaggc
     5341 gactatggaa ggcgactgag aggcggaata cgactgagaa ggggaagatg acggcgaagg
     5401 ctttgagaag agtgcgcgtg cggctcggcg gtggcgcgga gtactcgatt gagatcggcg
     5461 ccggggcgct cgaccatttg ggcgaggcgg cccggcgcgt cctccacgcg aaggcgcgac
     5521 gcgtcgcgct cgtctcgaac cggcttgtgt tcgacctcta cggcgcgcgc gccgaggaga
     5581 gtctgcgcgc ggccggcttc acggtcgcgc actggctgat gggcgacggc gagcgccaca
     5641 agacgctacg cacagccgag cgcgctctgg aatttctgtc ggcggccggg attgaacgct
     5701 cggacgcggt cgtggcgctc ggcggcggcg tggtcggcga cctcgccggt ttcgccgcgg
     5761 ccctgcacct gcgcggcgtc gcgttcttac aagcgccgac cacgctgctc gcgcagatcg
     5821 actcgtcggt cggcggcaag accggcgtca atacacacgc gggcaagaac ctcgtcggcg
     5881 tcttccacca gccgcgcgcc gtcgtcgtgg acacggagac gctgcggaca ttgcccgcgc
     5941 gcgagttgac cgccggctgg tgcgaggccg tcaagcaggg cgcggcgggc gacctcaaac
     6001 tcttccggcg gactgtcaag tttcttgccg aagaaagtga ggaggcgcgc tcgcgcgagg
     6061 atgagttgat tgagttgatc gccgcacatt gtgagttcaa ggcgcgcatc gtggcgggcg
     6121 acgagcgcga agacctcgac cgcgcggacg ccggctcgcg gcgcatcctg aacttcggcc
     6181 acaccgtcgg gcacgcgctc gaagcagtta ccggctttcg acgtttccgc cacggcgagg
     6241 cggtcggtta cgggatgctc gtcgccggcg agatttccaa aaagttagga atgctcgcgg
     6301 cttcggagtt agaatgcctg cgcgaagcgg ttcggcttgc cgggcgtctg ccacgcgccg
     6361 gagacatcag cgaagagcga atccggcacg cgcttctcag ggacaagaag tcggtcaacg
     6421 gccgcgtcct ttgggttctg ctcgaagggc ttgggcgcgc ggcgctcgtc gacggcagcg
     6481 aagtctccgc gcgcgttctg cgcgacgcac ttggggccac gctcgcgacg cgctccgcca
     6541 agtcgtaaga agggaaaccc gatatgaagc accaagctga cacacgccgg cgggggttcg
     6601 ccgacgagcg cggcgcgggg cgtctgagct tctacctcgt cgtcctcctg ctcgcggcag
     6661 ccggctacgt cgcctatcag gtagtgcccg tcttataccg cgcctccacc tacaaggtct
     6721 acatgcagga cgcggtgtct aagggcgcgg ctcttgggaa ggacccggag tgggtcaaga
     6781 cgcagctcaa aaccgccggc gtcgaagagt atggcctgcc gccgggcgca ctcgtcgaga
     6841 cgcacgtggc cgaaggccgc atcaacgcgc gcgtgcgcta cacgcgcccc atcgacctca
     6901 ccgcctacgt ctacgagtac aacttcgacc acgccgtcaa gagcgacaag ttcctcaaca
     6961 cgcaataaaa gggagtgata agtgatgagt gataagtgat aagagaaagc atccgccttt
     7021 tcttatcact tatcactcat cacttatcac tccccttctg ccttgcttca ggcagcggcg
     7081 ccggtgagct ggacgagtcc gggcggcgcg gcggcaagcc aggtggtggg gcagacgccg
     7141 cagcgcgtgc aggtgtccac cgaggggcag ggcgcggtcg atttggcgct ctgtgcgcgc
     7201 tcgtgctcgc gctccatgaa ctcgcgaccg aggccggcgt ccacgatctc ccaaggcagc
     7261 acctcgtcgt aggtgcgccg gcggccggtg tggaagtcgg cgtcgaagtt cgtttcacgc
     7321 agggcggcct gaaggtcgcc gcggtggcgc gtcatggact cgatgacgcg gccgaccgag
     7381 c
//
LOCUS       DASVES010000498         7427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1464686, whole genome shotgun sequence.
ACCESSION   DASVES010000498 DASVES010000000
VERSION     DASVES010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464686"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="waaF"
                     /locus_tag="VF240_20970"
     CDS             <1..966
                     /gene="waaF"
                     /locus_tag="VF240_20970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX7177745.1"
                     /translation="RPWAEGIFEGADFIDELLPLSGKSKGATALLSQARELRRRRFDL
                     ALLLPNAFAPALATFAARVPLRVGYATDGRRTLLTHPLAAPSWRGERHEVYYYLNLVA
                     ELERLLHGRKTAVVSEPRGEPQPRGELAVSDERRRAARALLRETGAREELPLVAICPG
                     STNSRAKRWPAERFAAVADMLCVRDGAQVVLIGAAEERDVSEEVARHMTQRPLILTGR
                     TSLAETAALLAVADLLVTNDTGPAHLAAAVGCPVVVIFGPTNPLTTRPFSESAEIVRH
                     PPDCAPCMLRDCPIDHRCMTAITPEEVYGRASVIVARARRFEVAR"
     gene            963..1544
                     /locus_tag="VF240_20975"
     CDS             963..1544
                     /locus_tag="VF240_20975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7177746.1"
                     /translation="MTERRAVFIDRDGTISEEVGYVNHPSRYRVFPYAAEAIKLLNDA
                     GWLAVLVTNQAGVARGYFAEEMIGAVHDRLARELERGGARLDAVYYCPHHPTVGAPPY
                     RQTCDCRKPRPGLIRRAADDLRIDTAASWMVGDRYGDVELARNAGLRSAFVLSGYGLG
                     EWEHQRPTWTHQPDLVADNLLEAVREILGRENG"
     gene            1662..2684
                     /locus_tag="VF240_20980"
     CDS             1662..2684
                     /locus_tag="VF240_20980"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7177747.1"
                     /translation="MARSFAGRRVVVLGDLVADQFVYGEISRVSREAPVLILRHERTE
                     TVPGGAANCAANLASLGARARVVGTVGDDAAGGALLERLNEMGVDCGGVAILPGAQTT
                     TKVRLLAGHAHSTRQQVIRMDYEGATPAGADAGAQIVEHLRLACADAEAVIVSDYNYG
                     VAGAEAVEVLRASFVGEGRPVLVDSRFRLPEFAGFTSATPNEDEVEHVAGRRLETLEA
                     LEAAGRELRERLRMKALLITRGSRGVMLVEEGAAPVHIEAVGALEAVDVTGAGDTVIA
                     AYALALASGASFADAARLANHAGGLVVMKRGTACVRLDELLASVSRREAEQAPAIRQD
                     SFSKSE"
     gene            2694..3200
                     /locus_tag="VF240_20985"
     CDS             2694..3200
                     /locus_tag="VF240_20985"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX7177748.1"
                     /translation="MEHTEEQPWVSAGARILERNRLIARVAIARKGGARVVFANGCFD
                     VLHVGHVRYLESARALGDLLVVGINSDEQVRALKGEGRPFVPERERAEVIASLRAVDF
                     VTVFHEPTVEKLLLAIRPNIHAKGTDYTEDTVPERDIVRSYGGRVCIVGDPKDHSSTQ
                     LVRVISDE"
     gene            3275..4372
                     /locus_tag="VF240_20990"
     CDS             3275..4372
                     /locus_tag="VF240_20990"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7177749.1"
                     /translation="MNILFVKLGSIGDIVHTLPALAAVRRALPEAEISWVVERRAAEI
                     LRDNPAVKNLIVIDTKALRRRPVSGETLLATRQQLRRLRASAFDFSLDFQGLLKSATI
                     ARLSGARRRFGFAHQHLREPASRFLLSDTVQTPARTHVIRKNLALAAGALGIDVPAEA
                     DEFEFPIGLRPEHECEADGIAARVGSPFAILNPAGGWPTKLWSPERYGALADQIFERH
                     GLRSAVTHGPGELELAKTVEKASRLGVAHAATPSLRGFYALARRTALYVGGDTGPTHL
                     AVAAGAPVVGLFGPTEWWRNGSPRPEDICVERTDIDCRTDCHRRSCSKWICMDIEVER
                     VAGAVSERLRRAGMVAAAGAPTRAERLTSGA"
     gene            4362..4955
                     /locus_tag="VF240_20995"
     CDS             4362..4955
                     /locus_tag="VF240_20995"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX7177750.1"
                     /translation="MARRGGTWMQRWRVPLGFLCGPVFILLAEPRPWTLAVGGCVAIA
                     GLALRAWAAGHIRKNAQLAISGPYAHTRNPLYLGSFLIGLGFTVAAGRPLLLGALFAA
                     LFLGIYLPVMRVEAATLGELFGDEYRAYARAVPLYIPRLTPYAEGASRQVRFDAGLYM
                     RYREYRAALGLALAWGMLALRDYGVLAFKALLANFAN"
     gene            4964..5902
                     /locus_tag="VF240_21000"
     CDS             4964..5902
                     /locus_tag="VF240_21000"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX7177751.1"
                     /translation="MKRLSTMNRLRTALAAVALLFAVLTPAYAAASGSDAAPTRRESP
                     APLPFEPTEQLVYEGSFSKLLLRGIKIAEFRFTSGRPDEAQLKSVSAGASNVAASTAA
                     ADSAARVHLIGDVKSAGWFQRLFSIDFHFRVESLVERAGFSVLSTKKLDEQGKRVRTS
                     EAMFDRVGKQVSWTEVDPNDPKREPRIVNTPLEGAAQDIVSAVYFLRTQQLTPGRSFE
                     IVLSDSGQVYRVPATVVAEPKRIKTVLGKVSVVRVEVALFGEGRPVEGEGKMSIWFTD
                     DARRVPVRARMSSDIGMIDIKLKSVSTNPPVQAQNR"
     gene            6217..>7427
                     /locus_tag="VF240_21005"
     CDS             6217..>7427
                     /locus_tag="VF240_21005"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7177752.1"
                     /translation="MVERRAPAGTRKTPRAKALAPATLEGPTVPREELAPLLALRCHD
                     PHATLGAHPVEGGFVVRAFRPEAERIEVVIKGRRKPFEMTRTHPAGFFELLLRGQAEA
                     FAYTLRVHYAGERLFVARDPYAFLPTVGTIDEHLFGEGRHERLYEKLGAHVRAFGSVR
                     GVSFAVWAPAAAGVSVVGDFNNWDGRLHQMRRLGGSGIWEIFIPDLPPGDAYKYEIHR
                     PGWPPLLKADPYAQFSELPPATSSVVFESAYEFTDDEWLRRRDGADHLRRPMSIYEVH
                     LGSWRRVLEEDNRSLTYRETAPALADYCDEMGFTHVEFLPLKGHPYGGSWGYQVTSYF
                     APTARFGTPDDFRYLVDYLHNRGIGVIMDWVPAHFPKDAWALGRFDGTALYEHLDPRL
                     GEHPDWGTYIF"
BASE COUNT         1159 a         2624 c         2397 g         1247 t
ORIGIN      
        1 cgcccgtggg ccgaggggat tttcgagggc gccgacttca tcgacgaact cctcccgctc
       61 agcggcaagt ctaagggcgc caccgccctc ctctcacaag cgcgcgagct gcgccgccga
      121 cgcttcgacc tcgcgctcct cctccccaac gccttcgcgc ccgccctcgc caccttcgcg
      181 gcgcgcgtgc ccctgcgcgt cggctacgcg acggacgggc gccgcacgct tctcacgcac
      241 ccgctggccg cgccctcgtg gcggggcgag cggcacgaag tttattacta cctcaacctc
      301 gtcgccgagc ttgaacgatt gctccacggc cggaagaccg cagtcgtgag cgagccgcgc
      361 ggcgagcctc agccgcgcgg cgagttggcc gtctccgacg agcgacgacg agcggcacgt
      421 gcgcttttgc gcgagacggg tgcgcgcgag gagttgccgc ttgtcgccat ttgtcccggc
      481 tcgaccaaca gccgcgcgaa gcgttggccc gccgagcgct tcgcggctgt cgccgacatg
      541 ctgtgtgtgc gcgacggcgc gcaggtcgta ctcatcggcg cggccgaaga gcgcgacgtg
      601 tccgaagagg tcgcgcgcca catgacgcaa cgccctttga ttcttaccgg gcgcacgagt
      661 cttgctgaga cggccgccct gctcgccgtc gccgatttgc tcgtgacgaa cgacacgggg
      721 ccggcgcacc tggcggcggc cgtcggctgc cccgtcgtcg tcatcttcgg cccgaccaac
      781 cccttgacca cgcgcccctt ctccgagtcg gccgagatcg tccgacaccc gcccgactgc
      841 gccccctgca tgctgcgcga ctgccccatc gaccaccgct gcatgacggc catcacgccc
      901 gaagaggtct acggccgcgc ttccgtaatc gtggcccgcg cgcgccggtt cgaggtcgcc
      961 cgatgacgga aagacgcgct gtcttcatcg accgcgacgg gacgatctcc gaggaggtcg
     1021 gctacgtcaa tcacccgtcg cgctaccgcg tcttccccta tgcggccgag gcaatcaaac
     1081 ttttgaacga cgcgggctgg ctcgccgtcc tcgtcacgaa tcaggccggc gtcgcccgcg
     1141 gctacttcgc cgaagagatg atcggggccg tccacgaccg tttggcgcgc gagcttgagc
     1201 gcggcggcgc gcgcctcgac gccgtctact attgcccaca tcaccccacg gtcggcgcgc
     1261 cgccctatcg acagacttgc gactgtcgca aaccgcgccc cggcctcatc cgccgcgccg
     1321 ccgacgacct tcgcatcgac acggccgcga gctggatggt cggcgaccgc tacggcgacg
     1381 tggagctggc gcgcaacgcc ggcctgcgct ccgccttcgt cctctccggc tacggcctcg
     1441 gcgaatggga gcaccagcgg ccgacctgga cacaccaacc cgacctcgtc gccgacaacc
     1501 tgctcgaagc cgtgcgcgag attttaggaa gggagaatgg ataagtgata agtgatgagt
     1561 gataagtgat gagaaaaagc tttgctgtta gctgttagct tatagctctt atcacttatc
     1621 acttataact catcacttac cacttcatta tgcttgaggc gttggcgcgt tcgtttgcgg
     1681 ggcggagggt cgtggtgctg ggcgacctcg tggcggatca gttcgtctac ggggagatca
     1741 gccgcgtctc gcgcgaggcg ccggtgctca tcctgcggca cgagcggacg gagacggtgc
     1801 ccggcggcgc ggcgaactgc gcggccaacc tcgcctcgct gggcgcgcgc gcgcgcgtcg
     1861 tcggcacggt cggcgacgac gcggccgggg gcgcgttgct cgaacgcctg aacgagatgg
     1921 gtgttgactg cggcggcgtg gcaatccttc cgggcgcgca gaccacgacg aaggttcgcc
     1981 tgctcgccgg ccacgctcac tcgacgcgcc agcaggtcat ccgcatggac tacgagggcg
     2041 caacgcccgc cggagcggac gccggcgcac aaatcgtcga gcatctgcga ctcgcctgcg
     2101 ccgacgcgga ggctgtcatc gtctccgatt acaactacgg cgtcgccggc gcggaggccg
     2161 tcgaggtttt gcgcgcgtcg ttcgtcggcg agggtcggcc cgtgctcgtc gattcgcgtt
     2221 tccggctgcc ggagttcgcg gggttcacgt cggcgacgcc gaacgaggac gaggtcgagc
     2281 acgttgcggg ccggcggctg gagacgctgg aggcgctgga ggcggcgggc cgtgagctgc
     2341 gcgagcgtct gcggatgaag gcgttgctga ttacgcgcgg gagccgaggc gtgatgctcg
     2401 tcgaggaggg agccgcaccg gtgcatatcg aggcggtggg cgcgcttgag gccgtggacg
     2461 tgacgggcgc gggtgacacc gtcatcgccg cctacgccct cgccctcgcc tccggcgcgt
     2521 ccttcgccga cgccgcgcgg ctcgccaacc acgccggcgg cctcgtcgtc atgaagcgcg
     2581 gcacggcctg cgtccggctc gacgagctgc tggcctccgt ctcgcgacgc gaggcggagc
     2641 aggcgccagc aattcgacag gattctttct ctaagtcaga ataggcgtct cggatggagc
     2701 acacagaaga acagccgtgg gtcagcgcgg gggcgcgcat actggagcgc aaccgtctga
     2761 tcgcgcgcgt ggcgattgcc cgcaagggcg gcgcgcgcgt cgtcttcgcc aacggctgct
     2821 tcgacgtgct gcacgtcggc cacgtccgct atctggagtc ggcgcgggcg ctgggcgacc
     2881 tgctcgtcgt cggcatcaat tccgacgagc aggtgcgcgc gctaaaaggt gagggccgtc
     2941 cgtttgtgcc tgaacgcgag cgcgccgagg tcatcgcctc cttgcgcgcg gtcgatttcg
     3001 tcaccgtctt ccacgagccg acggtcgaga aactccttct cgccatccgc cccaacatcc
     3061 acgccaaggg caccgactac accgaagaca ccgtccccga gcgcgacatc gtccgctcct
     3121 acggcgggcg cgtctgcatc gtcggcgacc cgaaggatca ttcgagcacg caattggtca
     3181 gagtgataag tgatgagtga taagtgataa gaaagagaac aagtcgcctc tcttccttct
     3241 tatcacttat cactcatcac ttatcaccgc ctttatgaac attcttttcg tcaaactcgg
     3301 gagcatcggc gacatcgtgc atacgctgcc cgccctcgcg gccgtgcggc gggcgttgcc
     3361 ggaggccgaa atttcgtggg tggtggagcg ccgcgcggcg gaaattttga gggacaaccc
     3421 ggccgtaaaa aacctcatcg taatcgacac gaaggccttg aggcgccggc cggtgtctgg
     3481 ggagacgctg ttggcaacgc gccagcagtt acgccggcta cgcgcctcgg ccttcgattt
     3541 ttctttagac ttccagggac tcctcaaatc cgcaaccatc gcgcgccttt cgggtgcgcg
     3601 ccgccgcttc ggctttgcac accaacacct gcgtgagccg gccagccgct ttctgctgtc
     3661 cgacaccgta cagacgccgg cccgcaccca cgtcatccgc aaaaatctcg cgctcgccgc
     3721 cggcgcgctc ggcatcgacg tgcccgccga ggccgacgag tttgaattcc ccatcggctt
     3781 gagacccgag cacgagtgcg aggccgacgg catcgccgcg cgcgtcggct cacccttcgc
     3841 catcctgaac ccggccggcg gctggccgac gaaactctgg agccccgaac gctacggcgc
     3901 gctggccgac cagattttcg agcgccacgg cctgcgctcg gccgtcactc acgggcccgg
     3961 cgagctggag ctggcgaaga cggtcgagaa ggcaagccgc ctcggcgtgg cccacgcggc
     4021 gaccccttcg ctcagaggat tctacgcgct ggcgcggcgg acggccctct acgtcggcgg
     4081 cgacacgggg ccgactcatc tggccgtcgc cgcgggcgcg cccgtcgtcg gactgttcgg
     4141 cccaaccgag tggtggcgca acggcagccc gcgccccgaa gacatctgtg ttgagcgcac
     4201 ggacatcgac tgccgcacgg actgccaccg gcgctcgtgc tcgaagtgga tatgcatgga
     4261 catcgaggtc gagcgcgtgg cgggggccgt gtcggagagg ttgcggcgcg cagggatggt
     4321 cgccgcggcc ggagcgccga cgcgtgcgga gaggctgacg agtggcgcgt aggggcggga
     4381 cgtggatgca gcgctggcgc gtgccgctcg ggttcctgtg cggccccgtc ttcatcctgc
     4441 tggccgagcc gcgcccgtgg acgctcgccg tcggaggctg tgtcgccatt gccggtctcg
     4501 ccctgcgcgc gtgggccgcc ggccacatcc gcaagaacgc acagctcgcg atctccggcc
     4561 catacgcaca cacgcgcaac cccctttatc tcggcagctt tctcatcggc ctcggcttca
     4621 ccgtcgcggc cgggcggcca ctgctcctcg gcgcactctt cgcggccctc tttctgggca
     4681 tctatcttcc ggtgatgcgc gtcgaagctg cgacgctggg ggagcttttc ggcgacgagt
     4741 atcgcgccta cgcgcgcgcc gtcccgctct acatcccccg tttgactcct tacgcggaag
     4801 gcgcgtcgag acaggtgagg ttcgacgccg gtctttacat gcgctaccgc gagtatcgcg
     4861 cggcgctcgg cctcgcgctg gcgtggggca tgctggcgct aagggattac ggcgtgctcg
     4921 ccttcaaggc gctgcttgcc aacttcgcaa actgagttcc gaagtgaaaa gactttccac
     4981 gatgaaccga ctccgcaccg cgttggccgc cgtcgccctc ctgttcgccg tcttgacgcc
     5041 ggcctacgcc gccgcatccg gctccgacgc cgcgccgacg cgcagggagt cgcccgcgcc
     5101 gttgcccttc gagccgaccg aacagctcgt ctacgagggg agtttttcca agctgctcct
     5161 gcgcggcatc aagatcgccg agttcaggtt cacgtccggg cgaccggacg aggctcagtt
     5221 gaagagtgtg tcggcgggag cgtcaaacgt cgccgcttcg accgccgccg ccgattctgc
     5281 ggcgcgcgtc cacctcatcg gcgacgtgaa gtccgccggc tggttccaac gtctcttcag
     5341 catcgacttc cacttccgcg tcgagtcgct tgtcgagcgc gccggcttct cggtgctgag
     5401 cacgaagaag ctcgacgagc agggcaagcg cgtccgcacg agcgaggcta tgttcgaccg
     5461 cgtcggcaag caggtttcgt ggacggaagt tgatccgaac gacccgaagc gcgagccgag
     5521 aattgtcaac acgccgctcg aaggcgccgc gcaggacatc gtctccgccg tctacttcct
     5581 gcgcacgcaa cagctcacac cggggcgcag cttcgagatc gtgctcagcg actcggggca
     5641 ggtctaccgc gtgccggcga cggtcgtggc cgagccaaag cggataaaga cggtgctggg
     5701 caaggtgtcg gtcgtccgcg tcgaggtcgc gctctttggc gagggccgcc ccgtcgaggg
     5761 cgaaggcaag atgtcgatct ggttcactga cgacgcgcgc cgcgtccccg tccgcgcgcg
     5821 catgtcgagc gacatcggca tgatcgacat caagctcaag tccgtctcga ccaacccgcc
     5881 cgttcaagct cagaacaggt aatgcctcaa cgagccgcgc acgcggcggc agaatgtagc
     5941 cacgggcgtt agcccgtgga acagcgttaa gaaaaatccg aaagccccgg aggggcgaaa
     6001 gagtcttcac gttgggcgca gcgcccatcc agattctttc gcccctccgg ggctttgtgt
     6061 tatttcgccc cctgtcccca cggcttgcgc cgtgggctac attctgccgc cgcttgcgcg
     6121 gctcgttgat gctttattcg tccttgatgt ttccgtgtgc ggagggtaag ttgtgcccga
     6181 actcggaatc acaatccaac tcccggcaca cgacgcatgg tcgagcgacg agcaccagca
     6241 ggaacgcgga agaccccgcg cgcgaaagcg ctcgcgccgg cgacgctcga aggcccgacc
     6301 gtcccgcgcg aagaacttgc gcctctgctc gcgcttcgct gtcacgaccc gcacgccacg
     6361 ctcggcgcgc acccggtcga gggcggcttc gtcgtccgcg ccttccgccc cgaggccgag
     6421 cggatcgaag tcgtcatcaa gggccgccgc aagcctttcg agatgacgcg cacacacccc
     6481 gccggcttct tcgagctgct gctgcgaggg caggccgagg cgttcgccta caccctgcgc
     6541 gtccactacg caggcgagcg cctcttcgtc gcgcgcgacc catacgcctt cctgcccacc
     6601 gtcgggacta tcgacgagca ccttttcggc gaaggccgcc acgaacgtct ctatgagaag
     6661 ctgggcgcgc acgtccgcgc gttcggcagc gtccgcggcg tctccttcgc cgtgtgggcg
     6721 ccggccgccg cgggcgtgag cgtcgtcggc gacttcaaca actgggacgg ccgccttcac
     6781 cagatgcgac gcctcggcgg ctccggcatc tgggagattt tcatccccga cctcccgccc
     6841 ggcgacgcgt acaagtacga aatccaccgc cccggctggc cccctctgct caaggccgac
     6901 ccctacgcgc agttctccga gttgcccccc gcgacatcat cggtcgtctt cgagtcagcc
     6961 tacgagttca ccgacgacga gtggctgagg cggcgcgacg gggccgacca cctgcgccgc
     7021 cccatgtcga tctacgaagt ccacctcggc tcctggcggc gcgtgctcga agaggacaac
     7081 cgctcgctca cctaccgcga gacggcgccc gcgctcgccg actactgcga tgagatgggc
     7141 ttcacacacg tcgagtttct tccgctcaag ggccacccct acggcggctc gtggggctat
     7201 caggtcacgt cctacttcgc gcccaccgcg cgcttcggca cgcccgacga cttccgctac
     7261 ctcgtcgatt acctacataa ccgcggcatc ggcgtcatca tggactgggt gcccgcgcac
     7321 ttcccgaaag acgcgtgggc gcttgggcgt ttcgacggca ccgccctcta cgaacacctc
     7381 gacccgcgcc tcggcgagca ccccgactgg ggcacgtaca tcttcaa
//
LOCUS       DASVES010000499         6593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1662481, whole genome shotgun sequence.
ACCESSION   DASVES010000499 DASVES010000000
VERSION     DASVES010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662481"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VF240_21010"
     CDS             <1..524
                     /locus_tag="VF240_21010"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7177753.1"
                     /translation="LTRDLAQRFNRDYGETFRLPEVFIPKVGAKIAALDDPSKKMSKS
                     EENDNSRINMLDEPDTVRRKFRRAVTDSGSEIRFDESRPGVTNLLSVYQIMTGRAPAE
                     IESHFEGKGYGKLKEEVAEATVEYLRPFRERVLAITDEELNRVLADGRDRARSIAGET
                     LRRVYERLGIVGA"
     gene            551..1372
                     /locus_tag="VF240_21015"
     CDS             551..1372
                     /locus_tag="VF240_21015"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX7177754.1"
                     /translation="MEQPEAQDADTAATVAAAAEGGQINVAGARPEISTAQGGEELKL
                     VLGEFSGPLDLLLYLIRQEQVSIYDIPLARITNAYLGYLRMMQGMDIAVASDFLVMAA
                     QLIEIKSKMLLPVDPSLSPEDAEADDPRRELIDRLLEHQKYKAAAEMLWSRATVEQAV
                     YARAPIETDKTNPEVSAGAFDLLRVFQEILTRKKEEVLMEIERDEMTMTEMLERLRNM
                     VFSTGELNLRQFFEHTRSRRELVLAFLSVLELVRTTEVRLFQSETFGDIVARVSS"
     gene            1377..2198
                     /gene="scpB"
                     /locus_tag="VF240_21020"
     CDS             1377..2198
                     /gene="scpB"
                     /locus_tag="VF240_21020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX7177755.1"
                     /translation="MAKRTKRNEESEAPAVAEELPAELEGNDGADEDALDGGAEFTSD
                     DEPDDEDEPEFLVHDADAEDRGEERARQLEAMRAGVEAADEDGEVAPGGGQRSMAELM
                     SIIEALVFVSDEPLTAKDIAAVLKEERGWVQLGCEELAKEFNERNGGLMLREVAGGWQ
                     LATRPEHHEHIRAYLKSKPSAKLSLAALETLAVIAYKQPVTVPEILEIRGVQSSSAIK
                     TLLDKRLIVAKGRKEVVGRPMLYGTSKEFLLQFGLKDLTELPSIEDFEDLAGGGA"
     gene            2223..2771
                     /locus_tag="VF240_21025"
     CDS             2223..2771
                     /locus_tag="VF240_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177756.1"
                     /translation="MRALKNTLGCFCLSVLFTSACLGQAESAPAPASVAGGAKLAIKC
                     LGAGVKAGERVALPGVHREGEQSFDLIVCYLVETEEDRRRGDLLIEFVIHGSDAAPVR
                     LKRPAIKVGEELLCRTDRSAYQSESAAGNVTDAVVAQTLTAHDFEQLFTGKPVTVQFG
                     RVAFELTQGHLDALRKLLKSGQ"
     gene            2917..3729
                     /locus_tag="VF240_21030"
     CDS             2917..3729
                     /locus_tag="VF240_21030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7177757.1"
                     /translation="MEERLQKLIAAAGVASRRHAEEMIAAGEVTVNGKIVRELGTKAD
                     PARDHIKVRGRLINPSLAAREKVYVLLNKPRGYLSSASDPEGRPLVTELVPASLGRLH
                     PVGRLDFNTEGLLILTNDGELTNYVTSARNKVRKVYEVKVKGVPPEQAVARLRRGIRL
                     DDGVRTAPAEITLVEETGNNAWYEVVLREGRNQQIRRMFDAVGHSVVKLRRVRIGDIT
                     DEQLRTREFRMLAPQEVARLMGKGRGKTRAAKAGATTTAGAVKAASKGRLKR"
     gene            3994..4647
                     /locus_tag="VF240_21035"
     CDS             3994..4647
                     /locus_tag="VF240_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177758.1"
                     /translation="MSRSRKIILVLFIVSSIFGMWARYSYQQAEKRKREELFRRLSMH
                     NTLGTEAAPSAEPVVAETRDMFDSTLPQESLEQIKQAVGQDFRLLEVWFHEKGVFAKV
                     STDGQSVKEYRRSTNKKEIDGPLEVRLTGDGKLEDNLLKLSDVDLSLIPKLAKEAKER
                     AALPDSKASSAGFKLPFIRYEGEGPEWSVMVETGKPGENWQSKHVTFDAKGKFKKVF"
     gene            4839..5984
                     /locus_tag="VF240_21040"
     CDS             4839..5984
                     /locus_tag="VF240_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7177759.1"
                     /translation="MDFELNEEQQQIKASVREFAEGEIRSHVREWDESQHFPVELVPK
                     LAEQGFMGVIFPEEYGGAGMGYVEYVTIIEELSRVDGSVGISVAAHNSLCSNHIYMFG
                     TEEQKRKFLTPLARGEKLGAWGLTEPQAGSDASGTRTTAVKRDGGWVVNGSKNFITHA
                     IAGDTCVAVASTDRAMRSKGITAFIFEKGTPGFLPGKKEDKLGLRASETASVIFEDCH
                     IPDSNRLGPEGLGFIQAMQILDGGRISIAALAVGIAQGAYESAVKYSKERQQFGKPIS
                     EFQAIQFKLADMATQLEAARLLTYRAAAMKDAGRKMTKESSMAKLFASEMSVKVCEEA
                     IQIHGGYGYTKDYPPEKYWRDSKLCTIGEGTSEIQRLIIAKQILSAV"
     gene            5995..>6593
                     /gene="meaB"
                     /locus_tag="VF240_21045"
     CDS             5995..>6593
                     /gene="meaB"
                     /locus_tag="VF240_21045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX7177760.1"
                     /translation="MANDDLLSRVLAGEPRAVARAISKVEDGTAGAFELMKGVFPHTG
                     KGLVVGITGSPGAGKSSLVDKLAAFYRKGGERVGIVAVDPSSPFSGGAILGDRIRMQA
                     LGLDPGVFIRSMATRGQLGGLARATVDAVAILDAAGYRKIIVETVGVGQDEVEIVKTA
                     DVSVVVLVPGMGDDIQAIKAGIMEIGDIFVINKADREGVL"
BASE COUNT         1299 a         2027 c         2193 g         1074 t
ORIGIN      
        1 agctgacgcg cgacctcgca cagcgattca accgcgacta cggcgagaca tttcgcctgc
       61 ccgaagtctt catcccgaag gtcggtgcca agatcgcggc gctcgatgac ccgtcgaaga
      121 agatgtcgaa gtcggaggag aacgacaact cgcgcatcaa catgctcgac gagccggaca
      181 ccgtccgccg caagttccgc cgggccgtca ccgactcagg gagtgagatt cgcttcgacg
      241 agtcgcgccc cggcgtcacg aacctcctca gcgtctacca gattatgacg gggcgcgcgc
      301 ccgccgagat agaatcgcac ttcgagggca agggctacgg caagctcaag gaggaggtgg
      361 ccgaggccac ggtcgagtat ctgcggccct tccgcgagcg cgtcttggcc atcacggacg
      421 aggagttgaa ccgcgtgctc gccgatgggc gcgaccgcgc gcgctcgatt gccggcgaga
      481 cgttgcgccg ggtctacgaa cggctcggga ttgtgggggc ttgagaatct tggcgagcga
      541 gacaccagag atggaacagc ccgaggcgca ggacgcggac acggcggcga ccgtggcggc
      601 ggcggccgag ggcggacaga tcaacgtcgc cggcgcgcgc cccgagattt ccacggcgca
      661 gggcggcgag gagctgaagc tcgtcctcgg cgagttctcc gggccgctcg acctgctgct
      721 ctacctcatc cgtcaggagc aggtttcgat ctacgacatc ccgctggcgc gcatcacgaa
      781 cgcctacctc ggctacctgc gaatgatgca gggcatggac atcgccgtcg ccagcgactt
      841 cctcgtgatg gcggcgcagc tcatcgagat caagtcgaag atgctgctcc cggttgaccc
      901 gagtctttcg ccggaggatg cggaggccga cgacccgcga cgcgaactca tcgaccggct
      961 gctcgaacac cagaagtaca aggccgcggc cgagatgctg tggtcgcgcg cgaccgtgga
     1021 gcaggccgtc tacgcgcgcg cgccgattga gacggacaag acgaaccccg aggtgtccgc
     1081 cggggccttc gacctgctgc gcgtctttca ggagatactg acgcgcaaga aggaagaagt
     1141 gttgatggag atcgagcgcg acgagatgac gatgacggag atgctggagc gcctgcgtaa
     1201 catggtcttc tccacgggcg agctgaatct ccggcagttt ttcgagcaca cgcgcagccg
     1261 gcgcgagctg gtgctcgcct tcctctccgt gctcgaactc gtccgcacca ccgaagtcag
     1321 acttttccag agtgagacct tcggcgacat cgtcgcccgc gtgagcagtt aaaaagatgg
     1381 ccaagagaac gaagcgaaac gaagaaagcg aagcccccgc cgtcgccgaa gaattgccgg
     1441 cggagctgga aggaaacgac ggggcggacg aggacgccct tgacgggggc gccgagttca
     1501 cgtcggacga tgaaccggac gacgaggacg agccggagtt cctcgtccac gacgccgacg
     1561 ccgaagaccg cggcgaggag cgcgcccgcc agctcgaagc catgcgggca ggcgtggagg
     1621 cggccgacga ggacggcgag gtcgcgcccg gcggcggcca gcggagcatg gccgagctga
     1681 tgtcgattat cgaggcgctg gtcttcgtct ccgacgagcc gctgacggcg aaagacattg
     1741 cggccgtgct caaggaggag cgcggctggg tgcagttagg ttgcgaggag ctggcaaagg
     1801 agttcaacga gcgcaacggc gggttgatgc tccgcgaggt ggcgggcggc tggcagctcg
     1861 ccacgcggcc cgagcaccac gagcacatcc gcgcctacct gaagtcgaag ccgagcgcga
     1921 aactgtcgct cgccgctttg gagactctgg ccgtcatcgc ctacaagcag cccgtcaccg
     1981 tcccggagat tctggaaatc cgcggcgtgc aatcctcctc ggccatcaag acgctcctcg
     2041 acaagcgact catcgtcgcc aaagggcgca aggaggtcgt cggccgcccg atgctctacg
     2101 gcacgtcgaa agaatttctc ttgcagttcg gtctgaagga cttgacggag ctgccaagca
     2161 tcgaagactt cgaggacttg gccggcggcg gggcgtgagc cgctgtcgcg gaggagacga
     2221 cgatgcgcgc acttaagaac actctcggct gcttctgcct gtcggtgctt ttcacgtccg
     2281 cgtgtctcgg gcaggcggag agcgcgcccg caccggcttc cgtcgccggc ggcgctaagc
     2341 tcgcgattaa atgtctgggc gctggcgtga aagccggcga gcgggtcgcc ctgccgggcg
     2401 tccaccggga gggcgagcag agcttcgatc tcatcgtctg ctacctggtc gagacggagg
     2461 aggatcgccg gcgcggcgac ctgctcatcg agtttgtcat tcacggcagc gacgccgcgc
     2521 ccgtccgact caagcggccg gcgatcaaag tgggtgagga actgctctgc cgcaccgacc
     2581 gcagcgccta ccagtccgag agcgcggccg gcaacgtcac cgatgcggtc gtcgcgcaga
     2641 cgctcaccgc gcacgacttc gagcagcttt tcacgggcaa gcccgtcacc gtccagttcg
     2701 ggcgcgtcgc cttcgagttg acgcagggtc atcttgacgc cctgcggaag ctactcaagt
     2761 ctggacaata aagaaagatc tgatttgaga ttccacggag gcgcgcgcaa ggcagtagcc
     2821 cgaccgtgag ggagggcgtc acgtcgatgc gccctccctc acggtcgggc tactgccctg
     2881 tcctactccg agaagtttcc gccccgtaaa atcatcatgg aagaacgatt gcaaaaacta
     2941 atcgccgccg ccggcgtcgc ctcgcgccgc cacgccgagg aaatgatcgc ggcgggcgaa
     3001 gtgacggtga atggaaagat cgtgcgcgag ctgggcacga aggccgaccc cgcgcgcgac
     3061 cacatcaagg tgcgcgggcg tctcatcaat ccgagtcttg ccgcgcgtga gaaggtttac
     3121 gtgctgctca acaagccgcg cggctatctg tcgagcgcgt ctgacccgga gggccgaccg
     3181 ctcgtcaccg agcttgtgcc cgcctcgctc ggccgccttc accccgtcgg ccgcctcgac
     3241 ttcaacaccg agggcctgct catcctcacc aacgacggcg agctgacgaa ctacgtcacg
     3301 agcgcgcgca ataaggtgcg gaaggtttac gaggtgaagg tcaagggcgt gccgcccgaa
     3361 caggctgtcg cgcgtctgcg ccgcggcatc aggctcgacg acggcgtgcg taccgcaccg
     3421 gccgagatca cgctggtcga agagacggga aacaacgcgt ggtacgaggt cgtgctgaga
     3481 gaagggcgca accagcagat tcgtcggatg ttcgatgcgg tcggccactc ggtcgtcaag
     3541 ctgcggcgcg tccgcatcgg cgacatcacg gatgaacaac tgcggacgcg cgagttccga
     3601 atgctggcgc cgcaggaggt ggcgcggctg atgggcaagg ggcgcggcaa gacgcgcgcg
     3661 gccaaagccg gcgcaacgac gacggccggg gccgtgaagg cggcgagcaa ggggcgtctg
     3721 aaaaggtagg acttaacaaa actgtagggg cagggcttgt ccgcggccct tcctcacaga
     3781 agaattaaca tggatgggca ggatgatcag gataagagat ttgaaatttg aaatctcaaa
     3841 tcttgaatct caaatcagct ccttcttcat cctgcgtatc ctgtccatcc atgttaattg
     3901 ctctgtagct aagggccgcg gacttgccct gcccatacat aattgttcac cgccattggt
     3961 tctatcagtc gccttaaatc tcggaggacg cagttgagcc gaagtcgaaa aatcatcctt
     4021 gtgttgttca tcgtctcgag catcttcggg atgtgggcgc gctactccta ccagcaggcc
     4081 gagaagcgaa agcgcgagga gctgttcaga cggctttcga tgcacaacac gctaggcacc
     4141 gaggccgcgc cctcggccga gccggtcgtc gcggaaacgc gcgacatgtt cgacagcacg
     4201 ttgccgcagg agtcactcga acagatcaag caggcggtcg gccaggactt ccgtttgttg
     4261 gaggtgtggt tccacgagaa gggcgtcttc gcgaaggtct cgacggacgg gcagagcgtg
     4321 aaagagtatc gccgctcgac gaataagaag gagatcgacg ggccgctgga ggtcagactc
     4381 acgggcgacg gcaagctcga agacaatctc ttgaagctga gcgacgttga cttgtcgctc
     4441 atcccgaagc tggcgaagga ggcgaaggag cgcgccgcgc tgcccgactc gaaggcttct
     4501 tcggcgggct tcaaactccc cttcatcagg tacgagggcg aggggccgga gtggagcgtg
     4561 atggtcgaga ccggcaagcc cggcgagaac tggcagagca agcacgtcac tttcgacgcg
     4621 aagggcaagt tcaaaaaagt attctgagcg cggcgccttt tgtcgtatgc cggccgcgcc
     4681 ttcgcgcacc ccttccgcac ttcgtttaaa gtcagactcc aagtagaata atcaaatcaa
     4741 caacacgcgt cagcgcgcgg ccgaaggcta caggggttta cgccaccgcg cgcttttccg
     4801 tgtgtgaaat ctttttaacc gggcggggct tttatttcat ggactttgaa ctcaacgagg
     4861 aacaacagca gatcaaggcg agcgtgcggg agttcgccga gggcgaaatc cgctcgcacg
     4921 ttcgcgagtg ggacgagtcg cagcatttcc ccgtggagct ggtgccgaag ctcgccgagc
     4981 agggcttcat gggcgtgatc ttccccgagg agtacggcgg cgcggggatg ggctacgtcg
     5041 agtatgtgac gatcatcgag gagttgtcgc gggtggacgg ctcggtcggc atctcggtcg
     5101 ccgcgcacaa ttcactgtgc tcgaatcaca tctacatgtt cggcaccgag gaacagaagc
     5161 ggaagtttct cacgccgctc gcgcgcggcg aaaagctcgg cgcgtgggga ctgaccgagc
     5221 cgcaggccgg ctccgacgcc tcgggcacgc gcaccacggc cgtcaagcgc gacggcggct
     5281 gggtcgtcaa cggctcgaag aacttcatca cgcacgccat cgcgggcgac acctgcgtgg
     5341 ccgtcgcctc gaccgaccgc gcgatgcgct cgaagggcat caccgccttc atcttcgaga
     5401 aggggacgcc cggcttcctc cccggcaaga aggaggacaa gctcggcctg cgcgcctctg
     5461 agacggcgag cgtcatcttc gaggactgcc acatccccga cagtaatcgg ctcggccccg
     5521 agggacttgg tttcattcag gcgatgcaga ttctcgacgg cgggcgcatc tcgattgccg
     5581 cgctcgccgt cggcatcgcg cagggcgcat acgagtcggc cgtcaagtat tcaaaggagc
     5641 gacagcagtt cggcaagccc atctcggagt ttcaggccat ccagttcaag ctcgccgaca
     5701 tggccacgca gctcgaagcc gcgcgcctgc tgacttatcg cgccgcggcc atgaaggacg
     5761 cggggcggaa gatgacgaag gagtcgtcga tggcgaaact cttcgcctcg gagatgtccg
     5821 tcaaggtctg cgaggaggcc atccagattc acggcggcta cggctacacg aaggactacc
     5881 cgcccgaaaa atactggcgc gactccaagc tctgcaccat cggcgagggc acgtccgaaa
     5941 tccagcgact catcatcgcc aaacaaattc tgagcgccgt ctgaccttcc cgttatggcc
     6001 aacgacgatc tactgtcccg cgtcctcgcc ggcgagccgc gcgccgtcgc tcgcgccatc
     6061 tcgaaagttg aggacgggac ggcgggcgca ttcgagttga tgaagggcgt cttcccgcac
     6121 acgggcaaag gtctggtggt gggcatcacg gggagtccgg gggcggggaa gtcgtcgctc
     6181 gtggataagc tcgccgcgtt ctatcggaag gggggcgagc gcgtcggcat cgtcgccgtc
     6241 gatccgtcga gtccgttctc gggcggggcg attctgggag accgcatcag gatgcaggcg
     6301 ctcggcctcg accccggcgt cttcatccgc tcgatggcca cgcgcgggca actcggcggc
     6361 ctcgcgcgcg cgaccgttga cgccgtggcc atcctcgacg cggcgggcta ccggaagatc
     6421 atcgtcgaga ccgtgggcgt cgggcaggac gaggtggaga tcgtgaagac ggcggacgtg
     6481 tccgtcgtcg tgctcgtgcc cggcatgggc gacgacattc aggcgataaa agccggcatc
     6541 atggagatcg gcgatatttt cgtcatcaat aaggctgacc gcgagggcgt cct
//
LOCUS       DASVES010000500        18169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1662853, whole genome shotgun sequence.
ACCESSION   DASVES010000500 DASVES010000000
VERSION     DASVES010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18169
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662853"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VF240_21050"
     CDS             complement(<1..598)
                     /locus_tag="VF240_21050"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7177761.1"
                     /translation="MPFKLRSEFKPQGDQPAAIDALVRGLDGGVKDQVLLGITGSGKT
                     FSVATVIDRVQRPALVIAHNKTLAAQLYQEFKSFFPENSVEYFVSYYDYYQPEAYVPA
                     SDTYIEKESTVNEEIDRLRLAATRALFERRDVIVVASVSCIYGLGSPEQYGKKTIGIS
                     VGQSLDRRKFLLGLSDMQYARNEIALERGNYRVRGDVIE"
     gene            complement(718..1365)
                     /locus_tag="VF240_21055"
     CDS             complement(718..1365)
                     /locus_tag="VF240_21055"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX7177762.1"
                     /translation="MSSNSPTTFLTGIDVSHYQGAIQWDEVKAAGYAFAFAKATEGAG
                     VVDPMFAANWNGMQRAGILRGAYHFYRAEQNPAAQAEHFVSTVQLAAGDLPPVIDIEV
                     NDGVVGTPLVSGVQTWLETVEQQTGVRPMIYTAASFWDAHFPAEFGQYPLWIAHYGPH
                     ATPLPGGWTTWAFWQYSQSIPAGGVQGAADHDYFNGQQSDLLALIAAARTPPQTT"
     gene            complement(1514..5428)
                     /locus_tag="VF240_21060"
     CDS             complement(1514..5428)
                     /locus_tag="VF240_21060"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7177763.1"
                     /translation="MPQNKPANPNRIRHRFAAQALSATLFVAAFISAAGDATTAHAQR
                     NRRPAPQGTQTTGAAPQQSQQPARPQAKLTPLRTSETSQGSRLTITSTNALSDYSAYR
                     SGDKFYVVIPNADADAVRGGARGRGYEGADVQKRGNDVVLSFKLKPGAKPRVNQRFNQ
                     LDVVFAATEGEGTAAQADGGGTSNRTQPSETQNASTTTTPPTPQPQQQDDRTAPSTST
                     TEQASAASNAARNSQATSNAPPAGAGTGTQPPIDINSLPPAGPVAAASPEATPADAPA
                     PETQIAQAAPPAGAAAPTSITAAPQVQPGTSIASAVLRNWPLTVIATLLLVGLGLFLA
                     ARRSSAAKQDPPAAPPLLEERKVAAIKEKPAPALKAVTTPATAKAAAPTTPKVAAPIT
                     PKAAVPTVSKVATPPAAPPPMSPLPTTVEAKTATEVAPAATEELVAPVVVAATTAVVS
                     PHKTSKAEKKKKRRGKKGAAATVVAATPATVEQLKEETAVQEAPEVTLVEEQTAEVST
                     TFEEPAAPQVEESFAPQIEESFAPEPAIDELPTATEPETVAEAEQLAAEMETVEETMP
                     VAETQFVAAAPSVEEVEPVTEIEPVSETLTEAEASAAAESVAIEPAAADEQTWAAPPA
                     ATAEIAPAAVELNAAVAHAETLRLLDGEAYDKSLAAVSDAMTRQIVASELLAALSGRN
                     AERRARAHTAFIEHGYFNEAAHNLSQAEAPVERASAARALGLAGDRHATPQLIEALED
                     PSMEVRRAAVESLAHVRDPRAVGALRALQEREKTERIKVPRRLIEHAVQVCEEAPAEV
                     EHTLAEDAAASLGAPAEEFVAAPAQEPPALVVPEETVVEEVAPAEALMPQADAAETFE
                     EAALYVETPEADAEASLFEPAAEVNTVPVEETPAAVEEFAPETDETVESPAFEEWAGT
                     EPAAVEEEAQPFVGFEEQPAAAEVPTELAAEDEIQLEEPATGIEAFTPTEELAEPVAP
                     AQLEFETYQAETVAERAVAPVEDFEATHTDAGAVAEEWIELDVEDVRVESGESEEASY
                     SRAVVPVEQLEAEETEAAEAGEFTASPSGGAPTVTEIEVAHLSSGVEEAAARFDNLTD
                     ARAPRDAGTPEGKGIAPFVEDDMSAVPKAIQMRLSSEEAEERAEAIKDLGRVDSGDAF
                     RVICSGFDDPSVEVRAAAARSLFGLQEDRAEAFTRALRESNPQRRRNIGAAIASSGLA
                     GEAISHLTGESRERTYDAFSLLFLMAKAGEVQPLIRAIEAHPDTEVRLAVVKLLALSG
                     QKEILPAFRRLAVRGTLPAEVRSAVMEAIYQISSSGQPTTTA"
     gene            complement(5514..8015)
                     /locus_tag="VF240_21065"
     CDS             complement(5514..8015)
                     /locus_tag="VF240_21065"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7177764.1"
                     /translation="MNTHTSWLKHVSPKVAGLRGARRARGLAVVALVVAPALFPQTAP
                     RAQRAKEEGASLKGVSASRQGGETTVSITGEGAMNRVQTWQDAEGFHVVFPNGKGAPG
                     RLPKGVKTQRVGNSLELVVPTRPGASVTVQPGAGRLDLVVSGDLDEGSGATEATPAAS
                     APAKSPRAETRERAAVEEQEQQQRRAEENAARQTSEAEDRATQKRDEPAAAGGNSSER
                     PNASEGGAPTAAQAATAANSDTIPNPNAAPAPPPLDATKAAAQTKIDARGGDSSFLSA
                     TGLLVLLSVAGLGGGLYFVRRRRASGASGGISKDKKAKKEKEKKSNAPAKQDALVKVE
                     AAKPVEPFELPPPFEHTMGDRRRGSVPVAQDRRRSGAGATDVGLHYNLTASGLLAPDG
                     TAGEPARKPGAATQWSTPTVLFGAYRIEQEVCKLVQGQPHSIDVLASRATDDRRAIET
                     SLVKALNSHETGEDGHWRVRQALEDYGFVARQSASLLLSNDAYERVSAARVLGQIKSA
                     ASLPFLLEALYDMTPVVRTEVVATLGSLGLPRAIGALIDMARRYPEVPESLIAPALTA
                     CSFEAQEWAWNALTDGRANDITGEHSAGGPSRPDSWVGVEELPEWLEAESLADAIEHL
                     ESADMEARISAAQSLAQYPARRSVDALTAILVRDENPAVRAAAVTSLAAIDHETVFAP
                     VLYALADEAREVRAAAARAYSGATYDRADASARLAETGDRETLEDLARACVKSGMAAQ
                     AVGRLASEDGRQAYEAFSMLSLVVRGGETAPVIETVAGHRDMSVRLAAVRLACLTGDA
                     GLLKSLRELAASGRLPENLRAAILDASEQNAQTHR"
     gene            complement(8213..8989)
                     /locus_tag="VF240_21070"
     CDS             complement(8213..8989)
                     /locus_tag="VF240_21070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7177765.1"
                     /translation="MTSKPAAHTANSNDGEEFRPEAGDRKLNLLVIEDDEKILEAISE
                     YFSRAGYSVHTAEDGVAGVQAALNDRPDAIVLDLMLPKMDGLAVCRELREKAPYIPIL
                     MLTAKDDVVDKVLGLEMGADDYITKPFSLRELEARIKSVLRRVRQATRTETTGDEAPI
                     LRGRLRIDPAKREVTIGDRLVDLTPKEFELLRLFASNPGRVFPRKYLLEKIWDYSYEG
                     YDRTIDSHINRLRAKIEENPENPQMVLTVWGIGYKFGDER"
     gene            9414..10322
                     /locus_tag="VF240_21075"
     CDS             9414..10322
                     /locus_tag="VF240_21075"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7177766.1"
                     /translation="MNGTQTVSETTLPGLSLLRRGKVRDVYEVDEERLLVVATDRVSA
                     FDCILPTQIPRKGEVLTALSRFWFERLANVAEHHLITTEIGEMPEPVRRHAETLRGRS
                     MLVRRTEVFPVECVVRGYLSGSGWKDYQRTGEVCGIRLPVGLRESDKLPEPLFTPATK
                     AEEGHDENISFARMSDIVGGQDAQALRRLALALYHEAEEHARARGIIIADTKFEFGRD
                     PRGRVLLIDEALTPDSSRFWPAEAYRPGGAQPSFDKQFVRDYLETLAWDKRPPAPPLP
                     PEVVEATTERYLQAYRLLTGDELHSP"
     gene            10422..10679
                     /locus_tag="VF240_21080"
     CDS             10422..10679
                     /locus_tag="VF240_21080"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7177767.1"
                     /translation="MQMRARLEALIDEMLDGQIMLDEALAEFEKLYIQKALARHREHL
                     SQTASTLGIHRNTLSKRVATYRTQERAARPPAHRPNSRPRR"
     gene            10756..11655
                     /locus_tag="VF240_21085"
     CDS             10756..11655
                     /locus_tag="VF240_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177768.1"
                     /translation="MDERSVAEAAEAAIKDLQANCQVSSVSHPSGKSGWCIQFTDGYG
                     QLCDDFQDEFGGDDSFLLRREKVKRHLLKTQDKMRRQLGRRRRGNLHTGEDQMATKRK
                     QDRSTLDTASKIVSDLMDTAGRAASTVVDQVSGLANTARETAASAASTVSPTAARTMR
                     PKREGSRSGSSRSVSSTAERAVDRGARQTRRAVSQATKTASSAARRTADAADRAAGRA
                     TKSAAKAAKKTVRAASGSSKSGSKKSGAKKSAGKKSVAKSVAKSASKSKAGSRKSAAG
                     KGAKRSASGAKKSGAKKSGARMR"
     gene            12061..12390
                     /locus_tag="VF240_21090"
     CDS             12061..12390
                     /locus_tag="VF240_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7177769.1"
                     /translation="MATNIRPLHDRVILRRIEEGEQVRGGIIIPDTAKEKPQEGEVIA
                     AGAGKYKEDGTRQELDVKPGDRVLFGKYSGSEIKIDGEELLIMREDEILGVIERAGAA
                     SGKKSGK"
     gene            12464..14107
                     /gene="groL"
                     /locus_tag="VF240_21095"
     CDS             12464..14107
                     /gene="groL"
                     /locus_tag="VF240_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7177770.1"
                     /translation="MAKQVVHGEESRAAILRGVNQLADAVKITLGPKGRNVVLDKKFG
                     SPTITKDGVTVAKEIELKDSMENMGAQMVREVASKTSDVAGDGTTTATVLAQAIFREG
                     VKTVAAGANPMALKRGIDKAVERAVKEIERMSKDVKGDMIAQVGTISANGDRTIGELI
                     AEAMKTVGKDGVITVEESRTMETELDVVEGMQFDRGYLSPYFVTDPERMEAVLDSPLI
                     LIHEKKVSSMKDLLPLLEQIAKLGKPLLIIAEDVEGEALATLVVNKLRGTLNVCAVKA
                     PGFGDRRKAMLEDISILTGGKVISEDLGIKLENVKLEDLGRAKKVTVDKDNTTLVEGA
                     GKSSDIEGRVKTIRAQVEDTTSDYDREKLQERLAKLVGGVAVIKVGAATETELKEKKA
                     RVEDAMHATRAAVEEGIVAGGGVALVRAAKALDKFKANEDGEGDSDEQIGVNIVRRAL
                     EEPLRQIAANAGVEGAVVVERVRSAKGENDGFNAATEKYEDLVKAGVIDPAKVTRTAL
                     QNAASIAGLMLTTEALVSELPEDDKAPAMPGGMGGGMGM"
     gene            complement(14234..14836)
                     /locus_tag="VF240_21100"
     CDS             complement(14234..14836)
                     /locus_tag="VF240_21100"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX7177771.1"
                     /translation="MPVLLRLMSIEIEKKYRLTRDQKETLARRLAEIGATRKGAEEFE
                     ENVIYTGPGLDPRSRVLRLRLTDARATFTYKERRATESAIKHQREEETEVSDAGALAS
                     ILDALGYSPALVYEKRRETWRAAGAEIVLDELPFGTFVEIEGEEESILKAEELLGLSS
                     AEAEHASYPELTARLGKNNGGVFEARFTQSALSDEQCRER"
     gene            complement(15562..16890)
                     /locus_tag="VF240_21105"
     CDS             complement(15562..16890)
                     /locus_tag="VF240_21105"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177772.1"
                     /translation="MSKLLSIIKREYVQGVRSKTFVVSTILGPAMLFVFAVVPSLLFG
                     MKAGGATRVAILDETGRLYESVRESVTRKDEGESTPGAGRGPESAARRAGRDMEASYV
                     VEQVALPPGGSLEEARRDLNGRIKSQTLDAYVILPRDIMETGRARFYSRSLGDVISIA
                     RLEDRLSRAVKEQRMRDSGIDPGVVQSVNREVEMSRHKPGEEGEEGTESSLFLALTVG
                     MFIFIATMMYGQAILSAVVEEKTTRIAEMLFSSTDAFTLMAGKLVGVSLLGLTQYALW
                     ALLFVGVSVFGVGAFSAGGMDFTLPNIPPVLALYALLFFLLGFYIYATMYAVVGAMVS
                     SEKEGAQLAYAVMMLPMAAIYLAFPVIRSPGSDFAFWVSMIPFFSPITMIVRIVAETP
                     PFWQIALSLAVGLATVVVLVWVAARIYRTGMLMYGKRATIPEIIRWARRA"
     gene            complement(16887..17807)
                     /locus_tag="VF240_21110"
     CDS             complement(16887..17807)
                     /locus_tag="VF240_21110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7177773.1"
                     /translation="MSDPSQPTLLLDRVTKSFDEFVAVDDLSIAVRPGRIFGLIGPNG
                     AGKTTTIRMIVGITAPDSGRVEIFGRPIGPEMQNRIGYLPEERGLYKKMKVGEQLRFF
                     GELKGMAGSEADRAADRWLERMKLSEWRKKKTDELSKGMQQKVQFIATIMHDPDLLIL
                     DELFSGLDPVNAGLMEEIVLELKAAGRTIIFSTHMMEAAEKLCDDICLLNRSHKVLEG
                     SLRGVREGFGRNAVALRMEGGDGVLDDAALVASVKPYGDYSEVLLAEGADSQELLRRL
                     VERGARVSRFERVEPSLRDIFITKVGEHNA"
BASE COUNT         3018 a         5944 c         6022 g         3185 t
ORIGIN      
        1 cctcgatcac atcgccgcgg acgcgatagt tgccgcgctc cagtgcgatt tcgttgcgcg
       61 cgtattgcat atcggagagg ccgaggagga acttgcggcg atcgagcgac tggccgaccg
      121 aaatgccgat cgtcttcttg ccgtactgct cgggcgaccc caggccgtag atgcaggaga
      181 cggaggcgac gacgatcacg tccctgcgct cgaagagggc gcgggtggcc gcgaggcgca
      241 ggcggtcgat ctcctcgttg accgtcgatt ccttctcgat gtaggtgtcc gaagccggga
      301 cgtaagcctc cggctggtaa tagtcgtagt aggagacgaa gtactcgacc gagttttcgg
      361 ggaagaaact tttgaactcc tgatagagtt gcgcggcgag cgtcttgttg tgggcgatga
      421 cgagggcggg gcgctgcacg cggtcgatga cggtggccac cgagaaggtc ttgcccgagc
      481 cggtgatgcc gagcaacacc tgatccttga cgccgccgtc gagtccgcgc accagcgcgt
      541 cgatggccgc cggctgatcc ccctgcggct tgaattcgga gcgaagcttg aaaggcataa
      601 ctcgtgaata gtaaatcgta aatcgtgaat cgtaaaacgg ccgttagcag ttagctttca
      661 gctattagct gagagatgtt ctacgattca cgatttacga tttactgttc acggttttca
      721 tgtcgtttgc ggcggcgtgc gggcggcggc gatgagggcg aggaggtcgg actgctggcc
      781 gttgaagtag tcgtggtcgg ccgcgccctg aacgccgccg gcggggatgc tctgcgagta
      841 ctgccagaag gcccaagttg tccagccgcc cggcagcggc gtggcgtgcg ggccgtagtg
      901 ggcgatccag agcggatact gaccgaactc cgcggggaag tgcgcgtccc agaaggaggc
      961 ggcggtgtag atcatggggc gcacgcccgt ctgttgttcg accgtctcca gccacgtctg
     1021 caccccggag acgagcggcg tgccgacgac gccgtcgttg acctcgatgt cgatgaccgg
     1081 cgggaggtcg ccggccgcca actgcaccgt gctgacgaag tgctcggcct gagcggccgg
     1141 gttctgctcg gcccggtaga agtggtatgc gccgcgcaga atgccggccc gctgcatccc
     1201 gttccagttg gcggcgaaca tgggatcgac gacgcccgcc ccctcggtcg ccttcgcgaa
     1261 agcgaatgcg tagccggccg ccttcacttc atcccactgg atagccccct gatagtgtga
     1321 aacatcgatc cccgtcaaga atgtcgtcgg tgagttgctg gacatctgtg gcgtctcctg
     1381 tctcgtgtcg tttcggtgag gcgcgtttcg cggtgcggcc tcgtgtggaa agtgaacggg
     1441 cggcggacac cgttgttaag aacgtcgtcc cccgcccgtc tggttcgaga aaatgaattg
     1501 cccgccggcg gcgtcaggcc gtggtggtgg gctggccgct gctgctgatc tggtagatgg
     1561 cctccatgac ggccgagcgc acttccgccg gaagcgtgcc gcgcacggcg agacgacgga
     1621 aggccgggag gatttccttc tgacctgaga gcgcgaggag tttgacgacg gccagacgca
     1681 cctccgtgtc ggggtgggcc tcgatggcgc ggatgagcgg ctgcacctcg ccggccttcg
     1741 ccatcaggaa cagcagcgag aaggcgtcgt aggtgcgctc gcggctctcg cccgtcaggt
     1801 gcgagatggc ttcgccggcg aggcccgagg aagcgatggc cgcgccgatg ttgcgccggc
     1861 gttgcgggtt cgactcgcgc agggcgcgcg tgaaggcctc ggcgcgatct tcctgaagac
     1921 caaacagcga gcgggcggcc gccgcgcgca cctcgactga ggggtcgtcg aacccggagc
     1981 agatgacgcg gaaggcgtcg ccgctatcga cgcgtcccaa atccttgatg gcctcggcac
     2041 gctcctcggc ctcctcgctg gagaggcgca tctggatggc cttcggcacc gccgacatgt
     2101 cgtcttcgac gaacggcgcg atgcccttgc cctccggcgt gcccgcgtct cgcggcgcgc
     2161 gcgcgtctgt caaattgtcg aagcgcgcgg ctgcttcttc cacgccggac gagaggtgcg
     2221 cgacttcgat ctcggtgacc gtcggtgcgc cgccgctcgg cgaggcggtg aactctccgg
     2281 cctcggccgc ctcagtctct tccgcctcca actgttcgac ggggacgacg gccctcgaat
     2341 acgacgcttc ctccgattcg ccagactcga ctctcacgtc ttcaacgtcg agttcaatcc
     2401 attcttcggc gaccgctccc gcatccgtat gcgtcgcctc aaaatcctcg acgggagcca
     2461 cggccctctc cgcgacggtc tccgcctgat acgtctcgaa ttcaagctgg gccggcgcga
     2521 ccggctcggc caattcctcc gtcggagtga acgcttcgat gcccgtcgcc ggctcctcca
     2581 actgtatctc gtcttccgcc gccaactctg tcgggacttc cgccgcggcc ggctgttctt
     2641 caaatccgac gaaaggctgc gcctcctctt cgacggcggc cggctccgtg ccggcccact
     2701 cctcgaaggc cggtgattcg accgtctcgt ccgtctcagg cgcgaactct tcgacggccg
     2761 ccggcgtctc ctcgaccggg acggtgttca cttccgctgc cggctcgaag agactcgctt
     2821 cggcgtcggc ctcgggcgtc tcgacgtaga gggccgcctc ttcaaaagtc tccgccgcgt
     2881 cggcttgagg cataagcgcc tcggccggag cgacctcttc cacaaccgtc tcttccggga
     2941 cgacgagcgc gggcggctcc tgggcgggcg ccgccacgaa ctcctccgcg ggcgcgccga
     3001 gtgacgcggc ggcgtcttcc gccagcgtgt gctcaacttc tgcgggggcc tcctcgcaga
     3061 cctgcacggc gtgctcgatg agccggcgcg ggactttgat gcgctccgtc ttttcgcgct
     3121 cctgaagcgc cctgagcgcc ccgacggcgc gggggtcgcg cacgtgggcc agcgactcga
     3181 cggcggcgcg gcgcacctcc atcgaagggt cttcgagggc ttcgatcagt tgcggcgtgg
     3241 cgtggcggtc gccggccagc ccgagtgcgc gcgcggccga ggcgcgctcg acgggggcct
     3301 ccgcctgact caagttgtgc gcggcctcgt tgaagtagcc atgctcgatg aaggccgtgt
     3361 gtgcgcgcgc gcggcgttcg gcattgcgac cggaaagcgc ggccagaagt tccgacgcga
     3421 cgatctgacg cgtcatggcg tcggagacgg cggccagact cttgtcgtaa gcctcgccgt
     3481 cgagcaggcg aagcgtctcg gcgtgcgcga cggcggcgtt aagctccacg gcggcgggcg
     3541 caatctcggc agttgccgct ggaggcgcgg cccacgtctg ctcgtcggcg gcggccggct
     3601 cgatagctac agactcggcg gcggctgatg cttccgcctc cgtcagtgtc tctgaaacag
     3661 gctcgatctc ggtgacgggt tcgacctctt cgacggacgg agccgccgca acgaactgag
     3721 tctccgcgac gggcatggtc tcttcaactg tctccatctc cgcggcgagc tgctccgctt
     3781 ccgcgaccgt ctcaggctcc gtcgcggtcg ggagttcatc gatggccggc tccggcgcga
     3841 atgactcttc aatttgcggc gcgaacgact cttcgacttg cggcgcggcc ggctcctcaa
     3901 acgtagtcga gacctctgcc gtctgctcct cgacgagggt gacttccggc gcttcctgca
     3961 ccgccgtctc ttccttcaac tgctcgacgg tggcgggagt cgcggcgacc acggttgcgg
     4021 ccgccccctt cttcccgcgc ctcttcttct tcttctccgc cttggaagtt ttgtgcggag
     4081 acacgaccgc ggtcgtggcc gcgacgacga cgggcgcgac taattcctcc gtcgcggccg
     4141 gtgcgacttc cgtcgcggtc ttcgcttcaa ccgttgtcgg cagcggcgac atgggcgggg
     4201 gcgcggccgg cggcgtggcg accttcgaca cagttggcac ggcggccttc ggagttatcg
     4261 gcgcggcgac tttcggtgtc gtcggcgcag cggccttagc tgtcgcaggt gttgtgacag
     4321 ccttcagcgc gggcgccggc ttctctttga tcgcggcgac ttttctttcc tctaacagtg
     4381 gcggcgcggc ggggggatcc tgctttgctg ccgacgagcg gcgcgcggcg aggaagagtc
     4441 cgaggccgac gagcaggagc gtggcgatga cggtcagcgg ccagttgcga aggacggccg
     4501 aggcgattga cgtgcccggc tgcacctgcg gcgcggcggt aatgctggtg ggagcagcgg
     4561 cgcccgcggg cggcgctgcc tgtgcgatct gtgtttccgg ggccggagcg tcggctggcg
     4621 tcgcctcggg agaagcggcg gccacggggc cggcgggcgg aagcgagttg atgtcgatgg
     4681 gaggttgcgt accagtgccc gcgcccgccg gtggcgcgtt cgaggtcgcc tgactgttgc
     4741 gggcggcatt cgacgcggcc gacgcttgct cagtcgttga agtggaaggc gctgtccggt
     4801 cgtcctgctg ttgcggctgc ggcgtcggcg gggtcgttgt ggtagaggcg ttctgcgtct
     4861 cgctcggctg tgtcctgttg gacgtgccgc cgccgtcggc ttgcgcggcc gtgccctcgc
     4921 cctccgtcgc ggcgaagacc acgtcgagct ggttgaatct ctggttgacg cgcggcttgg
     4981 cgccgggctt gagcttgaat gagagcacca cgtcgttgcc gcgcttctgc acgtcggccc
     5041 cctcatagcc gcgaccgcgt gcgccgccgc ggacggcgtc ggcgtcggcg ttcgggatga
     5101 cgacgtagaa cttgtcgccg ctgcggtagg ctgaatagtc gctgagcgcg ttggtggagg
     5161 tgatggtcag acgcgagcct tgcgaggttt cgctggtgcg caggggcgtg agtttcgcct
     5221 gcggtcgcgc gggctgttgg ctctgctgcg gtgcggcccc ggtcgtctgt gtgccctgcg
     5281 gggcgggcct tcggttgcgc tgcgcgtgcg ccgtcgtcgc gtcgccggcc gcactgataa
     5341 acgcggcgac gaagagcgtc gcggagagcg cctgtgcggc aaagcggtga cgaattctgt
     5401 ttggattagc aggtttgttt tggggcattg cggtagctca acagtcaggg caagtgttcc
     5461 ggggcattat gcaatagcgg cgcgactttt gtcattaaac aattctccgc gccttagcga
     5521 tgcgtctgcg cgttctgctc ggaagcatcc agaatagcgg cgcgcaagtt ttcgggcaga
     5581 cggcccgacg cggccagctc gcgcaggctc ttcaacagcc cggcgtcgcc cgtcagacac
     5641 gcgagccgca cggccgcgag gcggacgctc atatcgcggt ggcccgcgac cgtctcgatc
     5701 acgggcgccg tctctccgcc cctcaccacc agcgagagca tcgagaaagc ttcgtatgcc
     5761 tgacgcccgt cttcgctcgc cagccggccg acggcctgcg ccgccatccc cgacttgacg
     5821 cacgcccgcg ccaaatcttc cagcgtctca cggtcgcccg tctcggccag acgggcggaa
     5881 gcgtcggcgc ggtcgtaggt ggcgccggag taggcgcggg cggccgccgc gcgcacctcg
     5941 cgcgcctcgt cggccagcgc gtagagcacg ggggcgaaga cggtctcgtg gtcaatggcc
     6001 gcgagactcg tgacggccgc cgcgcgcacg gccgggttct cgtcgcgcac gaggatggcc
     6061 gtcagcgcat cgacggagcg ccgcgccggg tactgcgcca gcgattgcgc ggccgagatg
     6121 cgcgcctcca tgtccgcgct ctccagatgt tcgatggcat cggccaaact ctcggcctca
     6181 agccactcgg gcagctcttc aaccccgacc cacgagtcgg gacgcgaggg gccgcccgcc
     6241 gagtgttcgc ccgtgatgtc gttggcgcgg ccgtccgtca gtgcgttcca cgcccactcc
     6301 tgcgcctcga aggagcaggc cgtgagcgcc ggggcgatga gcgactcggg gacttccggg
     6361 tagcggcggg ccatgtcgat gagcgcgccg atggcccgcg gcagcccgag cgagccgagc
     6421 gtggcgacga cctcggtgcg gacgaccggc gtcatgtcgt agagtgcttc gagcaggaac
     6481 gggagcgacg ccgccgactt gatctgtccg agcacgcgcg ccgccgagac tcgctcgtaa
     6541 gcgtcgttcg agagcagcag cgacgcgctc tgtcgcgcca cgaacccgta gtcttcgagc
     6601 gcctgacgca cgcgccagtg tccgtcctcg cccgtctcat gcgagttcag cgccttgacg
     6661 agagaggtct cgatggcccg ccggtcgtcg gttgcgcgtg aggcgagcac gtcgatgctg
     6721 tgcggctgac cctgcacgag cttgcagacc tcctgttcga tgcggtacgc gccgaacaga
     6781 acggtgggcg tgctccactg cgtcgccgcg cccggcttac gcgccggctc gccggccgtg
     6841 ccatcgggtg caagcaagcc gctggccgtc aggttgtagt gcaagccgac atcggtcgcg
     6901 cccgcgccgc tgcgccggcg gtcttgggcc accggcacgc tgccgcggcg gcggtcgccc
     6961 atggtgtgct cgaagggcgg cggcagctcg aacggctcga ccggcttcgc cgcctcgacc
     7021 ttcaccaacg catcctgctt cgccggcgca ttcgacttct tctctttctc tttcttcgcc
     7081 ttcttatctt tgctgatccc gccagacgcg ccggacgcgc gcctccggcg cacgaagtag
     7141 agacctccgc cgagacccgc gacggacaga agcacaagta gcccggtcgc cgagaggaat
     7201 gaggagtcgc cgccccgtgc gtcgatcttc gtctgtgcgg ccgccttcgt tgcgtcaagc
     7261 ggcggcggcg cgggggcggc gttcgggttg ggtatcgtgt ccgagtttgc ggccgtggct
     7321 gcctgcgcgg ccgtgggggc gccgccctcg gaagcattcg ggcgctccga agagttgccg
     7381 ccggccgccg ccggctcgtc cctcttctgc gttgcgcggt cttcggcctc cgacgtttgg
     7441 cgggcggcgt tctcctcagc gcgccgctgt tgctgctcct gctcctcaac ggcggcgcgc
     7501 tcgcgcgtct cggcgcgcgg cgatttcgcc ggagcgctgg cggcgggtgt cgcttccgtt
     7561 gcgccggagc cttcgtcgag gtcgccgctg acgacgaggt cgaggcggcc cgctcccggt
     7621 tgcacggtca cgctcgcgcc ggggcgggtc ggcaccacga gttcaagcga gttgccgacg
     7681 cgttgcgtct tcacgccctt cggcagacgc cccggcgcgc ccttaccgtt cgggaagacg
     7741 acgtgaaaac cttccgcgtc ctgccacgtc tgcacgcggt tcatcgcgcc ctcgccggtg
     7801 atcgagacgg tcgtctcgcc gccctggcgc gaggcgctca cgcccttgag gctcgctccc
     7861 tcctccttgg cgcgttgcgc gcgcggcgcc gtctgcggga agagtgcggg cgcgacgacg
     7921 agcgcgacga cggcgaggcc gcgcgcgcgg cgcgcgccgc gcaacccggc gactttcggc
     7981 gagacgtgtt tcaaccaaga ggtgtgcgtg ttcatggcgg tgattccggc ggacgcttgc
     8041 agcagcgtgc cttttgaaga gtgaggggtt gcggcctagc ctacaggatt cggcggcggc
     8101 ggcagcaggg atggtttgtg gacgcgggag gcggcgacgt ggggcgtgtt cagcttgtcg
     8161 ccgtgccgac taacgggcga gacgttttaa ggagagttgg gaactcaagc cttcagcgtt
     8221 cgtcgccgaa cttgtagccg atgccccaga cggtcagcac catctgcggg ttctcggggt
     8281 tctcttcgat cttcgcacgc aggcggttga tgtgcgagtc gatggtgcgg tcgtagccct
     8341 cgtaagagta gtcccagatt ttctcgagga ggtacttgcg tgggaagacc cggccggggt
     8401 tggaggcgaa cagccgaagc agctcgaact ccttcggcgt gagatcgacg aggcggtcgc
     8461 cgatggtcac ttcgcgcttc gccgggtcga tacgcagacg gccacggagg atgggagcct
     8521 cgtcgcccgt cgtctccgta cgggtcgcct gccggacgcg gcggagcacg cttttgatgc
     8581 gcgcctcaag ctcgcgcaag ctaaagggct tggtgatgta gtcgtcagcg cccatctcca
     8641 gaccgagaac tttatcgacc acgtcgtctt tcgccgtcag catcaggatc gggatgtaag
     8701 gggccttctc ccgcagctca cggcagacgg cgaggccgtc catcttcggc agcatcagat
     8761 cgaggacgat ggcgtcgggc cggtcgttca aagccgcctg cactcctgcc accccgtcct
     8821 cggccgtatg tacgctgtaa ccggcgcgcg agaagtactc gctgatggct tcgagaatct
     8881 tctcatcgtc ttcgatgacg agcaggttca gcttgcggtc gccggcctcg gggcgaaatt
     8941 cctccccgtc gttggagttg gcagtgtgtg cggcaggttt actggtcatt gattggccgt
     9001 cgtcgggcaa gggccggaac gaaggtttat aaggcagggg gcaccggcag ggacgccgcc
     9061 gcaccggcta cgttaaaaat ataacagtac agcggcggca aactcaagcc ctaagttgtc
     9121 tatttgctaa tgttcggtag cgggcttaac ccgccgcacc ggccctgcgt ctcgggccga
     9181 ccgtcaaggt ctattcatgc ttgagctaag gaaaggatga aggatgaggg atgagggatg
     9241 aaagaaggca tcttgtcttc acttcatccc tcatccctca tccttcatcc ttgctttccc
     9301 gccccgtatc ttcgttcttg acaaaaatcc ggcgacgcga gcacaatcgc ggctcgcctc
     9361 cggcgcccgc gcccaagcga cccgaccaag ccaccgcgcc gcgcccccct tttatgaacg
     9421 gcacccagac ggtctccgag accacactcc ccggactctc gctcctgcgc cgcggcaagg
     9481 tgcgcgacgt gtacgaagtt gatgaagagc gcctgctcgt cgtcgccacc gaccgcgtct
     9541 cggccttcga ctgtatcctg cccacgcaga ttccccgcaa gggcgaagtg ctcaccgccc
     9601 tctcccgctt ctggttcgag cgtcttgcca atgttgccga gcaccacctc atcacgaccg
     9661 agatcgggga gatgcccgag ccggtgcggc gtcacgccga gaccctgcgc gggcgctcga
     9721 tgctcgtgcg ccggacggag gtcttccccg tcgagtgtgt cgtgcgcggc tacctctccg
     9781 gctcaggctg gaaagactac cagcgcaccg gcgaagtctg cggcatacgc ctccccgtgg
     9841 gactgcgtga atccgacaag ctcccagaac cgctgttcac gcccgcgacg aaggccgaag
     9901 aggggcacga cgagaacatc agcttcgcgc gcatgtccga catcgtgggc gggcaggacg
     9961 cgcaagccct gcgccgcctc gcgctcgcgc tctaccacga ggcggaggag catgcgcgcg
    10021 cgcgcggcat catcatcgcc gacacgaaat tcgagttcgg gcgcgacccg cgtgggcgcg
    10081 tgctcctcat cgacgaggcg ctgacgcccg actcctcgcg cttctggccc gccgaagcgt
    10141 accgccccgg cggagcgcaa ccctccttcg acaagcagtt cgtgcgcgat tatctggaga
    10201 cgctcgcgtg ggacaagcgc cccccggcgc cgccgctccc gcccgaggtc gtcgaggcca
    10261 ccacggagcg ctaccttcag gcttaccgtc tactgacggg cgacgaactt cacagcccgt
    10321 gactctccac gcctttgcgg cgctcgacga ctgttttcgc gttcgatagt ttttgcgttc
    10381 ggccccgtgt tgaagtatcc cccatactgg agcctcaagc catgcagatg cgcgcccgcc
    10441 tcgaagcctt aatcgacgag atgctcgacg gccagatcat gctcgacgaa gccctcgccg
    10501 agttcgagaa gctctacatt cagaaggcgc tggcgcgcca ccgcgaacac ctctcgcaga
    10561 cggcctccac gctcggcatc caccgcaaca cgctctcgaa gcgcgtcgcc acctaccgca
    10621 cgcaggagcg cgccgcccgt ccgcccgcgc atcgtcccaa ctcccgcccg cgccgctgaa
    10681 attttttatc acccgcgctc gctgttttga agtattatcg ccgacaagtt ttcgcaagag
    10741 ccaaatcgaa ttcacatgga cgagcgctca gtcgcagagg ccgccgaggc cgcgatcaaa
    10801 gacctccagg ccaactgcca agtcagctct gtctcgcacc cctcgggcaa gagcggctgg
    10861 tgcattcagt tcaccgacgg ttacggtcag ctctgcgacg actttcaaga cgagttcggc
    10921 ggcgacgaca gcttcctgct acgtcgggag aaggtgaaac ggcacctgct gaagactcag
    10981 gacaagatga gaaggcaact cggccgtcgc cgccggggaa acttacatac aggagaagac
    11041 cagatggcca ccaaacgtaa acaggaccgc tcgactttgg acaccgccag caagatcgtc
    11101 tcagacctca tggacacagc cggccgcgcc gccagcaccg ttgtcgatca ggtctcgggc
    11161 ctcgccaaca cggcccgcga gacggccgcc agcgccgcct ccaccgtcag cccgaccgcc
    11221 gctcgcacca tgagacccaa gcgcgaaggc tcgcggtcag gatcgagccg cagcgtctcc
    11281 agtaccgccg agcgcgccgt tgatcgcggc gcgcgccaga cgcgacgcgc tgtctcgcag
    11341 gcgaccaaga ccgcgagtag cgccgcccgg cgcaccgctg acgccgccga ccgcgccgcc
    11401 ggacgcgcga ccaagtccgc cgcgaaggcc gccaagaaga ccgtcagggc agccagcggc
    11461 tcgtcgaagt ccggcagtaa aaaatccggc gcgaagaagt cggctggtaa gaagtcggtc
    11521 gcaaagtcag tggcgaagtc cgcgtcaaag tcgaaggccg ggtcgaggaa gtcagcggcc
    11581 ggcaaaggcg caaagagaag cgcatccggc gcgaagaagt ccggcgcgaa gaagagcggc
    11641 gcgcgtatga gatagcagga tgcgcccgac cttcgtcgcc tcaaatcgca cccaccgccc
    11701 cgtctaaata gttccgaccc gtggcgggca cgtcttcacc ctgacgcgcc cgccacttcg
    11761 catccagcaa acacatcttc gccgcccgtt cccttcccgc cagagccgcc agctcctctc
    11821 agattgcccc ccgacgcgca ttttcaaacg cctttccagg cccctgattg acaagccacc
    11881 ccgtgttgca ttattgttag cactcgcccc gaggtagtgc taagaattgt gcgtttcgcc
    11941 agcaaaaagg cggtcacgct gtgcgcggac tcgggcgggt catcaagttc tgaagagaac
    12001 gttcttaagc aatcaaaccc acatcaccaa tcgagaggtg aatttcggaa aggagttagc
    12061 atggcgacca acatcagacc gcttcacgac cgtgtaatct tgcgccgcat cgaggagggc
    12121 gagcaggttc ggggcggcat catcattccc gacaccgcga aagagaagcc gcaggagggc
    12181 gaggtcatcg ccgccggcgc gggcaagtac aaggaagacg gcacccgcca ggagcttgac
    12241 gtgaagcccg gcgaccgcgt gctgttcggc aagtacagcg gctcggagat caagatcgac
    12301 ggcgaggagc tgctcatcat gcgcgaggac gaaatcctcg gcgtcatcga gcgcgccggc
    12361 gcggcctccg gcaaaaagag cggcaagtag tctcagaaga gactgcgggc cgctttttca
    12421 tttagagcat taccgataat tatagcttga ggagattact gtcatggcaa agcaagtagt
    12481 acacggagag gaatcgcggg cggcgatcct gcgcggcgtc aaccaactcg ccgacgcggt
    12541 taagatcacg ctcgggccca aaggtcgcaa cgtcgtcctc gacaagaagt ttggttcccc
    12601 gaccatcacg aaggacggcg tcaccgtcgc taaagagatt gagctgaagg actcgatgga
    12661 gaacatgggc gcgcagatgg tgcgcgaggt tgcctcgaag acttctgacg tggccggcga
    12721 cggcaccacc acggcgaccg tgctcgcgca ggccatcttc cgcgagggcg tgaagacggt
    12781 ggcggcgggc gccaacccga tggccctgaa gcgcggcatc gacaaggccg tcgagcgcgc
    12841 ggtgaaagag atcgagcgca tgtcgaagga cgtgaagggc gacatgatcg cgcaggtcgg
    12901 caccatctcc gccaacggcg accgcaccat cggcgagctg atcgccgagg cgatgaagac
    12961 cgtcggcaaa gacggcgtca tcacggtcga ggagtcgcgc acgatggaga cggagctgga
    13021 cgtggtcgag ggcatgcagt tcgaccgcgg ctacctctcg ccctacttcg tcaccgaccc
    13081 cgagcgcatg gaggcggtgc tcgatagccc gctcatcctc atccacgaga agaaggtcag
    13141 ctcgatgaag gacctgctgc cgctgcttga gcagatcgcc aagcttggca agccgctcct
    13201 catcatcgcc gaggacgtgg agggcgaggc tctcgcgacg ctcgtcgtca acaagttgcg
    13261 cggcaccctc aacgtctgcg ccgttaaggc ccccggcttc ggcgaccgcc gcaaggcgat
    13321 gctcgaagac atctcgatcc tgacgggcgg caaggtcatc agcgaagacc tcggcatcaa
    13381 gctggagaac gtcaagctcg aagacttggg tcgcgccaag aaggtgacgg tggacaagga
    13441 caacacgacg ctcgtcgagg gcgccgggaa gtcttcggac atcgagggcc gcgtcaagac
    13501 catccgcgca caggtcgagg acaccacctc ggactatgac cgcgagaagt tgcaggagcg
    13561 ccttgccaag ctcgtcggcg gcgtcgcggt gatcaaggtc ggcgcggcca ccgagacgga
    13621 gctgaaggag aagaaggcgc gcgtcgagga cgccatgcac gccacgcgtg cggcagtcga
    13681 ggaaggcatc gtcgccggcg gcggcgtcgc gctggtgcgc gcggcgaagg ctctcgacaa
    13741 gttcaaggcg aacgaggacg gcgagggcga ctcggacgag cagatcggcg taaacatcgt
    13801 gcgccgcgcg ctcgaagagc cgctgcggca gatcgcggcg aacgcgggcg tcgagggcgc
    13861 tgtcgtcgtc gagcgcgtgc gctcggcgaa gggcgagaac gacggcttca acgcggcgac
    13921 cgagaagtac gaagacctcg tcaaggccgg cgtcatcgac ccggcgaagg tcacccgcac
    13981 ggcgctccag aacgcggcct ccatcgcggg cctgatgctc acgaccgagg cgctcgtctc
    14041 cgagcttccc gaggacgaca aggccccggc gatgcccggc ggcatgggcg gcggcatggg
    14101 catgtaagcg gccccgccga aacgctgaaa gcggcgtcgc tctcaatgag agcgacgccg
    14161 ctttttttat caactgacag acagtaccgc gagcggtagc gagcgggtct ggttgcgaga
    14221 gcagtcccgc tcgctacctc tcgcggcact gttcatcact aagcgcgctt tgtgtgaacc
    14281 gcgcctcgaa cacgccgccg ttattcttcc cgagccgcgc ggtcaactcc gggtacgacg
    14341 cgtgttcggc ctccgccgac gagaggccga gcagttcttc cgccttcaga atcgactcct
    14401 cttccccttc tatctccacg aacgtcccga agggcagttc gtccagcacg atttccgccc
    14461 cggccgcgcg ccatgtctcg cgccgcttct cgtagacgag ggcggggctg tatccgaggg
    14521 cgtcgaggat ggacgccagc gcgccggcgt cgctcacctc ggtctcctct tcgcgctgat
    14581 gcttgatggc cgactcggtg gcgcggcgct ccttgtaggt gaaggtcgcg cgcgcgtcgg
    14641 tgagccggag tcggaggacg cggctacgcg ggtcgagacc ggggccggtg tagatgacgt
    14701 tctcctcaaa ctcctccgcg cccttgcgag tcgcgccgat ctcggcgagc cggcgcgcga
    14761 gcgtttcctt ctggtcgcgc gttagcctgt actttttttc aatctcaatg gacatcaggc
    14821 gtagtaggac aggcattcct gcctgtcagc ctgccgcagg caggcacaaa acttcggtaa
    14881 gaatgacctc cacattaaat cacaggtctg acagacattc ctgcctgtca gacctagccg
    14941 cgctcgacca gggccattcc tcagctttct cgacaagtcc ggctgagact ggatttctct
    15001 cgatgtaccg gatgattcgt tgagcctcca agtcgttacg aacccaatga tcgtaagact
    15061 catcctgcca gaatggccta cctgttcgtc ccagaatctt gtttgccttc cgggctgtat
    15121 aacctttgac agtctttgtg atctttggaa gcggaaccag ggggcggata aagatatgta
    15181 cgtggttcgc catgacggtg tatgcaagta ggcggtagag attcaggaga tgatctccga
    15241 aatgcagggc ttcaactatg cctccggcga ttggggggtt tttcagccag agcgggccgc
    15301 tcgccgcacg atccaaatgc ctgtcgaatg ccagaaaagc acggccggcg tccgtctctt
    15361 cacgccgagg cgtcctcaag ttcgcgggta acgagccgtg aagcctccac gtcacaaaga
    15421 gactggctcc ttctggcagc cagtgaggta ggtgccttcg atagaataac attgccatag
    15481 gacaggcatt cctgcctgtc gttttcaggt tctaatcgag ccgagcttat cacgtccacg
    15541 tgacaggcag gaatgcctgt cctacgcccg gcgcgcccaa cggatgattt cggggatggt
    15601 ggcgcgcttg ccgtacatga gcatgcccgt gcgatagata cgcgcggcca cccagacgag
    15661 gacgacgacc gtggcgaggc cgacggcgag cgagagcgcg atctgccaga agggcggcgt
    15721 ctcggcgacg atgcggacga tcatcgtgat gggcgagaag aacgggatca tcgagaccca
    15781 gaaggcgaag tcggagccgg ggctgcggat gacggggaag gcgaggtaga tggccgccat
    15841 cgggagcatc atgacggcgt aggccagttg cgctccctcc ttctccgagg acaccatcgc
    15901 gccgaccacg gcgtacatgg tggcgtagat gtagaagccg aggaggaaga agagtagcgc
    15961 atagagggct aagactggcg ggatgttcgg cagcgtgaag tccatccccc cggcggagaa
    16021 agcccccacc ccgaaaacgg acaccccgac gaacaggagc gcccacaggg cgtattgcgt
    16081 caggccgagg agcgagacgc cgaccagttt cccggccatc agcgtgaagg cgtcagtgga
    16141 cgagaagagc atctcggcga tgcgggtcgt cttctcctcc accacggccg agagaatcgc
    16201 ctggccatac atcatggtcg cgatgaagat gaacatgccg acggtgaggg ccaggaagag
    16261 gctgctctcc gtgccctcct caccctcctc gccgggcttg tgccggctca tctcgacctc
    16321 gcggttgacg gattgcacca cgccggggtc gatccccgag tcgcgcatcc gctgctcctt
    16381 gacggcgcga ctcaggcggt cttcgagccg cgcgatggag atcacgtcgc cgaggctgcg
    16441 cgagtagaag cgcgcgcggc ccgtctccat gatgtcgcgc ggcaggatga cgtaagcgtc
    16501 cagagtctgt gacttgatgc gcccgttgag gtcgcgccgc gcctcttcga gcgagccgcc
    16561 cggggggagg gcgacctgct ccaccacgta gctcgcctcc atgtcgcgcc ctgcgcgacg
    16621 ggcggccgac tccggcccgc gtcccgcacc cggcgtggac tccccttcgt ctttgcgcgt
    16681 gaccgattcg cgcaccgact cgtagagcct ccccgtttcg tccaggatgg cgacgcgcgt
    16741 cgcgccgccc gccttcatgc cgaagagcag ggaggggacg acggcgaaga cgaagagcat
    16801 cgcggggccg aggatcgtgg agacgacgaa cgtcttgctg cggacgccct gcacgtactc
    16861 gcgcttgatg atggagagga gtttgctcat gcgttgtgct caccgacctt cgtgatgaag
    16921 atgtcgcgca gggaaggctc gacgcgctcg aagcgggaga cgcgcgcgcc gcgctcgacg
    16981 aggcggcgca gaagctcctg cgagtcggcg ccctcggcga gcagcacttc cgagtagtcg
    17041 ccgtagggct tgacgctcgc gacgagcgcc gcgtcatcga gcaccccgtc gccgccctcc
    17101 attctgagcg ccacggcgtt gcgcccgaaa ccctcccgca ccccgcgcag gctcccctcc
    17161 agaactttgt gcgagcggtt gagcaggcag atgtcgtcgc agagtttttc ggccgcctcc
    17221 atcatgtgcg tcgagaagat gatcgtgcgc cccgccgcct tcagctccag cacgatctcc
    17281 tccatcagcc cggcgttgac ggggtcgaga cccgaaaaca actcgtcgag gatgagcaga
    17341 tcagggtcgt gcatgatggt ggcgatgaac tgcaccttct gctgcatccc cttcgacagc
    17401 tcatcggttt tcttcttgcg ccactcggag agcttcatgc gttcgagcca gcggtcggcc
    17461 gcgcggtcgg cctcactgcc cgccatgccc ttcagctcgc cgaagaagcg gagctgctcg
    17521 ccgactttca tttttttgta gaggccgcgc tcctcgggca ggtagccgat gcggttctgc
    17581 atctcggggc cgatgggtcg cccgaagatt tcgacacggc cggagtcggg ggccgtgatg
    17641 ccgacgatca tgcggatggt ggtggtcttc cccgccccgt tcggcccgat cagaccgaag
    17701 attcttccgg gccggacggc gatgctcaga tcgtcaacgg cgacgaactc atcgaagctt
    17761 ttggtgacgc ggtcgaggag gagggtgggc tggctggggt cgctcacttg gtggattttt
    17821 acctccggcg ggggcaaccc gtttgcgccg cggcgcgcat ctcacgggcg agggttaaaa
    17881 actcgtggga ttatacggga gacgggcggc gaaagtttag acgcccccaa aggccgctcc
    17941 gcttttccgg gtctgacgat gtgttaagat gcacgggccg caagtctccc gcccggaaac
    18001 acgaagggtt tctcggttat gccgtccgct tcctctcaac tcaaacgctc ccgcgcctcg
    18061 ctcttcttca cacttctgct cctctgcacc cccgcgcccg cccagcaaac gccgcagacg
    18121 cccgcccccg cccaggagcg ggacgaagtt atccgcatca gcaccgaga
//
LOCUS       DASVES010000501        13223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1666533, whole genome shotgun sequence.
ACCESSION   DASVES010000501 DASVES010000000
VERSION     DASVES010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666533"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VF240_21115"
     CDS             complement(<1..951)
                     /locus_tag="VF240_21115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177774.1"
                     /translation="MNDPLTEKADATAHREDGHTVSGRAAPRPELSSAAPAAISVEHV
                     THVYPVPLARLKQLLRRSVNSTQALTDVTLNVREGEIFGLIGRNGAGKTTLTKIISTL
                     IQPTTGTVSVRGFDGVREEERVRAQTGLASAEERSFYWRLTGEQNLLFFARLYGMRTR
                     DARRRISELLSLFELEPAARKRFGELSTGNKQRMSIVRAMLARPPVLLLDEPTRSLDP
                     VAAARMRSIIGELARATPPVSVLLTSHNLAEVEELCERVAVISRGRIRAIDTPAALRS
                     LNRQRETVSLRLTRTTSERAESVLAGALDDLAVSDESGGSV"
     gene            complement(960..1793)
                     /locus_tag="VF240_21120"
     CDS             complement(960..1793)
                     /locus_tag="VF240_21120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177775.1"
                     /translation="MNFELDMNRLAYFGIFFVAVLVIVAAFAPVIATHDPGASDILAL
                     NLKPSAAHWFGTDAIGRDVFSRVVYGARISLTVGIVVVAVSSVVGTVIGALAGYYGGW
                     IDSIVSGYLFNVFLAFPGLLLAIALVAFLGGGLNKLILALCIIGWVGYARMIRGQVLK
                     VREYDFVQAARALGAGGLRILFVHILPNAIQPLIVQASLGMAGAVLSEAALSFLGLGL
                     PPRAPSWGRMLEEARSITVLERAPHTMIFPGAAVALTVLAFNFLGDGLREYLDPRQRK
                     R"
     gene            complement(1860..2852)
                     /locus_tag="VF240_21125"
     CDS             complement(1860..2852)
                     /locus_tag="VF240_21125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177776.1"
                     /translation="MKYKLQDILRRALVLLLVVWTVVSLVTILIELVPGDPAAAILGD
                     QARPEDLVAFRTRHGLDRPAFFFTFPETAGGARTFRWHGWDNRYVEYWRGILKGDMGL
                     SFRTDRPVRALILSRYGATIQLAVAALLVAVAIAVPLGVVAGTNRGNWLDNVLSVVAL
                     FGISLPSFVLGPLLMFVFAVWLGWVDPSGRFEWKSIILPAVTLGAALSALLTRMVRSS
                     VIEEMGEDYVRTARAKGLSERKVVYKHVLKNGLIPVVTILGLQLGVLLAGAIITEKIF
                     NWPGIGLLLVEEGINKRDYRVVQGCVLVISVTYILANTLTDLLYRRLDPRIRLS"
     gene            complement(3157..3900)
                     /gene="ubiE"
                     /locus_tag="VF240_21130"
     CDS             complement(3157..3900)
                     /gene="ubiE"
                     /locus_tag="VF240_21130"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX7177777.1"
                     /translation="MLASRQQLQVTGSEGEHARRVRGMFARIAGRYDLLNHLLSGNTD
                     RRWRRQVAKRLCAALAPDARVLDIACGTGDLALAVLEAAPGARVVGVDFCRPMLELAA
                     RKAGTDGRRVPFVEGDALRLPFADGAFDGVTIGFGLRNLASVEEGLREIRRVLAPGGA
                     AAILEFSRPVVPGFRALFQFYFSRLLPAVGGLLSGSRAAYEYLPDSVRNFPDQERLAA
                     MMREAGFEQVSFENLTGGIAALHVGRREA"
     gene            complement(3961..5373)
                     /locus_tag="VF240_21135"
     CDS             complement(3961..5373)
                     /locus_tag="VF240_21135"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7177778.1"
                     /translation="MAGPLAVLLFFLAALPANCAAQAVPAPPAGAEQREQLLNGLLIV
                     LVERPGEAQTVLRLRLHNGASFDLAGKDGLMALLGDALFPDPQTREYVTGDLEGRLSV
                     SVGHDSIDVLLAGRADQFERLVELLRNAVVNTQLTPEVFGRVRDARVKIVRELGVSPG
                     AVADDAISARLYGSYPYGRRVAGTPESLARVERGDLVQARERFLIPNNATLIVSGGFE
                     RRRALRALRQFLGVWRKGDQIVPPTFRQPEAVDPRPLVVDFPNLPDAEVRVAVRGVAR
                     SERDAATGAVLAELVRSRWLAAVPELKGRAVTVRHDARALDGAFLLGASVPTTSAAAQ
                     ALEAARKVMESLAATAVPADELETAKKSVVAAFNKQAESGEQLADDMLDSQTYGTAQA
                     SMSEMARAAGSLTPADVQRVASKLFRVQAASVVVGDAARLRADLARLGEVETSGDATA
                     KPTPKPAQASPFKSPTQRRP"
     gene            5906..7783
                     /locus_tag="VF240_21140"
     CDS             5906..7783
                     /locus_tag="VF240_21140"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7177779.1"
                     /translation="MDASALNVAETAPAPEPSSPAIPAYVADERTTVVSAAAVNVASV
                     ETPAADHVAASASGSNGNTPSPSASVPASIDPEETAVSQAFAPRGEYHLTMLQDEGIT
                     RRLSKELRETAHEYELTWPEFKRDPGGFTKRMATGYGQMGRRFFNQPYVPMAVISGVL
                     GMAILVTLLVFPFNAVYKWLASDGRLVADVGMTLEEVQRRSTLKLAPAAAQGDGSTLT
                     TVSGENLGFDFELANKGLKFEWARAYLLSVNEEQKIKEINVELSRTPQAWRELATSVR
                     NLDESLEDRGWRVFTEGESNTENAKFLKASLAEQQTAPPAEGDPGELRWIDRDASNVI
                     TFTAKRAEGGGEARFVSYLKVEELTRETLVAMLEIPEEQEKPDEGPAGRAKGTGGGMK
                     PKQEKPGGGGGGGRKEALPASFGKVPPGALQPQIVAITPHPPAIKNPSLPVTPHIIAD
                     PTLFPPDNRPIPYGDPKSTSTTPSAGPGEGAGIGDGKGTGVGPGEGAGFGPGRGYNTG
                     GGDGKIGGGGPGGGGGGTDYNKTFNAKDVTRKAVLNSKPEPGFTEEARKNNVTGVVKL
                     RLVLGAGGSVSNISVVKGLPDGLTERAIAAARRISFTPAQKDGRPVSQWVTIEYNFNI
                     Y"
     gene            7935..8747
                     /locus_tag="VF240_21145"
     CDS             7935..8747
                     /locus_tag="VF240_21145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7177780.1"
                     /translation="MLRRVFLNTYLRAEAEAREQRLPGRFQGVDLKVISVCIIVALSL
                     TMINYVGELVYVLDALDRLGPAGADERLWSGLTNFSNERLAGPIWWACVTVVFYFVVP
                     ALFIRLALKEPLSDYGLKLRGAFKDYGLYLLMFVVMFPVVLLVSGAAGFQAKYPFYQM
                     ARGESLLPYFWQWEVFYFLQFFALEFFFRGFMLHGLKHRFGYYAVFVMTMPYCMIHFR
                     KPFLEALAAIIAGVILGTLSLKSRSILPGVAIHYSIAIVMDLAALWRKNLLW"
     gene            8807..9547
                     /locus_tag="VF240_21150"
     CDS             8807..9547
                     /locus_tag="VF240_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177781.1"
                     /translation="MAFWARAKVAVRKTDLAASGLFVQSFNVVKEAANRVLGLPDLAG
                     QVLGLAPVKTLRLRVVILRDEQSRSLAEAREVMPSVEAARAIFERQARIKVSPARGSF
                     IQTLDTAAPADALDVHCDFHAWKEGLREAGNFFETYLGQHAAVMLTGYAAPITVFIVR
                     DVSGKGGCSLGPLTDYVTVAICGLDSCGEDEEGRAVARLLAHEVGHACGLWHVRDPSN
                     LMHPRGPGVKLRPWQRAILRNSRHVTYL"
     gene            9653..10885
                     /locus_tag="VF240_21155"
     CDS             9653..10885
                     /locus_tag="VF240_21155"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7177782.1"
                     /translation="MSETPTRVENVVCVASFFKGADFLRECRARGARVFLVTRERTLG
                     EEWPREAIDDLVAIPNDASLDLFLYAVSHLARRVRIDRVVALEEFDVVNTALLREQLQ
                     VPGMHGTAARAFRDKLTMRVRAREAGIRVPDFVHVLNHEALHAFMTRVSAPWVLKPRS
                     DVSSIGIKKLHDPEQVWRAIDELDARGALHERSPYYVLEQYVAGEVFHVDSLVEDGEV
                     TFAGVNRYGRPPLDVAQQGGVYISHTLDYDSEDRARLEEFNRRLLKSLRLESGAAHAE
                     FIKGEADGEFYFLEVASRVGGAYMAETLEAASGVNLWREWARLELAGGSRAAEERPQP
                     RREYGGIALSLARQEWPDTSQYTDAEIVGRVRKAYHVGLVVGSPSLARVEELLDDYAR
                     RFAADFCAVAPPHERPPL"
     gene            10918..12855
                     /locus_tag="VF240_21160"
     CDS             10918..12855
                     /locus_tag="VF240_21160"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7177783.1"
                     /translation="MSQIKVAPYGSWESPITSELIVSATVGLGQCLIDGDDLYWSEMR
                     PLEGGRSVVVRRTPDGRTADVTPAGFNARTRVHEYGGGDYTVRAGIVYFSNFADQRLY
                     RVSAGEQPAPLTPAGDYRYADCLLDSRRGLLYAIREDHSDKTREAVNCVVCVRADGDE
                     SGGRVLVSGNDFYSSPRLSPDGRRLAWLTWNHPNMPWDGSELWVGELDDDGLVRSSRK
                     VAGGVDESIFQPEWSPDGTLYFSSDRTGWWNLYRETVGEGVEPICGRESEFGQPQWGF
                     GMSIYAFADSGRIVAARVERGTARLCVIDTATKELRDAETPYTDITYVRASDGRVVFR
                     GASATERAGIVALDLDTGRTDVLRRSSEQVIDAGYVSAASAVEFPTEGGLTAHAFYYP
                     PRNRDYAAPDGERPPLLVKCHGGPTAATVPSLRLETQYWTSRGFAVLDVNYGGSSGYG
                     REYRRRLNGEWGIVDVDDCVNGARHLVGRGEADGERCAITGGSAGGFTVLNALTFREQ
                     FRAGASHYGVSDLKGLIEDTHKFEARYLDRLIGPYPERADLYRERSSINFTERLSCPV
                     IFFQGLEDKVVPPNQAELMVEALRKKGLPVAYVPFEGEQHGFRMAENIRRALDGEFYF
                     YSRVFGFEPAERIEPVLIENL"
BASE COUNT         2326 a         4303 c         4449 g         2145 t
ORIGIN      
        1 gacacttccg ccgctttcgt ccgagacggc gaggtcgtcg agcgcgcccg cgagcacact
       61 ctcggcgcgc tcgctggttg tgcgggtaag ccggaggctg acggtctcgc gctgtcggtt
      121 gagcgagcgc agggccgccg gcgtgtcgat ggcgcggatg cgcccgcggc taatgacggc
      181 cacgcgctca cagagttctt ccacctcggc gagattgtgg gaggtgagca ggacgctgac
      241 gggcggcgtg gcgcgcgcca gctcgccgat gatcgaacgc atgcgcgcgg cggcgacggg
      301 gtcgagcgag cgcgtcggct cgtcgagcag gaggacgggc gggcgggcga gcatcgcgcg
      361 gacgatggac atgcgctgct tgtttccggt ggacaactcg ccgaaccgct tgcgtgcggc
      421 cggctcaagc tcgaagaggg agagcagttc actgatgcgc cggcgcgcgt ctcgcgtcct
      481 catgccgtag agccgtgcga agaacaagag gttctgctcg cccgtcagac gccagtagaa
      541 gctccgctcc tcggcgctcg ccagccccgt ctgggcgcgc acgcgctcct cttcgcgcac
      601 gccgtcgaag ccgcgcacgc tgaccgtgcc cgtggtgggt tggatgagcg tggagataat
      661 cttggtgagc gtcgtcttgc ccgcgccgtt gcgtccgatg aggccgaaga tttcgccctc
      721 gcggacgttg agagtaacgt cggtcagagc ctgcgtgctg ttgacggagc ggcggaggag
      781 ctgcttgagg cgggcgagcg gaacggggta gacgtgcgtg acgtgctcga cggagatggc
      841 cgctggggcc gccgacgaaa gttcggggcg cggcgcggcg cgacccgaga cggtgtggcc
      901 gtcttccctg tgcgcggttg cgtcggcctt ttctgtgaga gggtcgttca tcggctcggt
      961 cagcgtttgc gctggcgcgg gtcgagatac tcgcgcaggc cgtcgccgag gaaattgaag
     1021 gcgagcacgg tgagggcaac ggccgcgccg gggaagatca tcgtgtgcgg ggcgcgctcc
     1081 agcaccgtga tgctgcgcgc ctcttcgagc atccggcccc acgacggcgc gcgcggcggc
     1141 agccccagcc cgaggaatga aagcgccgct tccgacaaca ccgcgcccgc catgccgaga
     1201 ctcgcctgca cgatgagcgg ctggatggcg tttggcagga tgtggacgaa gaggatgcga
     1261 aggccccccg cgccgagcgc ccgcgccgcc tgcacgaagt cgtactcgcg caccttcaag
     1321 acctgcccgc gaatcatccg cgcatagccc acccagccga tgatgcagag cgcgaggatg
     1381 agcttgttta agccgccgcc taagaaagcc acgagcgcga tggcgagaag caggccgggg
     1441 aaggcgagga agacgttgaa caggtagcca gagacgatgc tgtcgatcca accgccgtag
     1501 tagccggcga gcgccccgat gacggtgccg acgacggagg agacggccac gacgactatc
     1561 ccgacggtca gagagatgcg tgcgccgtag acgacgcgcg agaatacgtc gcgcccgatg
     1621 gcgtcggtgc cgaaccagtg cgcggccgac ggcttaagat tgagcgcgag gatgtcgctg
     1681 gcgccggggt cgtgtgtcgc aatcaccggc gcgaacgccg ccacgatgac gagcaccgcg
     1741 acaaagaaga ttccgaaata ggctaagcgg ttcatgtcga gttcaaagtt caaagttcaa
     1801 agttcaaagt caaagaaaga ggttgttcct ttgaactttg aactttgaac tttgaactat
     1861 caagaaagcc ttatccttgg atcgagccgc cggtagagca agtccgtgag cgtgttggcg
     1921 aggatgtagg tcacgctgat gacgagcacg cagccctgca cgacgcggta gtcgcgcttg
     1981 ttgatgccct cctcgaccaa gagcaggcca atgcccggcc agttgaaaat cttctcggtg
     2041 atgatggcgc cggccagcag gacgccgagt tgcaggccga ggatggtgac gacgggtatc
     2101 agcccgttct tgagcacgtg tttgtagacc accttgcgct cgctgagacc cttggcgcgc
     2161 gccgtgcgga cgtagtcctc gcccatctcc tcgatgacgg aggagcgcac catgcgtgtc
     2221 aggagcgccg agagcgccgc gccgagtgtg acggccggca ggatgatgct cttccactcg
     2281 aagcgccccg acggatcgac ccacccgagc cacacggcga agacgaacat cagcagcggc
     2341 ccgaggacga agctcggcag gctgatgccg aagagggcga cgaccgagag gacgttgtcg
     2401 agccagttgc cgcggttcgt cccggccacg acgccgagcg ggacggcgat agctacggcg
     2461 acgaggagcg cggccaccgc cagttgaatc gtcgccccgt agcgcgacag gatcagcgcg
     2521 cgcacaggcc ggtcggtgcg aaacgacagc cccatgtcgc ctttgaggat gccgcgccag
     2581 tattccacgt agcgattgtc ccagccgtgc cagcgaaagg tgcgcgcgcc gccggccgtc
     2641 tcggggaagg tgaaaaagaa ggccgggcgg tcgaggccgt ggcgggtgcg aaaggccacg
     2701 aggtcttcgg ggcgcgcctg atcgccgagg atggccgcgg ccgggtcgcc cggcaccagc
     2761 tcgatcagga tggtcacgag ggagacgacc gtccagacga cgagcaggag gacgagcgca
     2821 cggcgcagga tgtcctgaag cttatacttc attcgcggcg gcgtaacgat tacgggaaat
     2881 taatttcgtc ggacgcgacg gatgatagca ccgaagcggc ggggccgtca gccctacacg
     2941 aaagaggcgg aagcccgacc gtcagggagg gcgtacttgg gcgtgagtat gccctccctg
     3001 acggtcgggc ttccgcccga ctttagaaag cacgtagccc gacggaactc tctccgaacg
     3061 acggccccga caaccaccga acgaatcaag cccatcgggc tacgtttggg gttgcgtatg
     3121 tttcaacgca accagtctcg gagggaggca gaagcgttac gcctcgcgtc ttccgacgtg
     3181 aagcgccgca atccccccgg tcaaattctc aaagctcacc tgctcgaacc ccgcctcgcg
     3241 catcatggcg gcgagtcgct cctggtcagg gaaattcctg accgagtccg gcagatattc
     3301 gtaagccgcg cgcgagccgc ttaacagacc gccgaccgcc ggcagcaggc gcgaaaagta
     3361 aaactgaaaa agagcgcgga agcccggcac gacgggtctg gaaaactcca gtatcgccgc
     3421 cgccccgccc ggcgcgagca cgcgccgaat ctcgcgcaga ccctcctcca cactcgcgag
     3481 gttgcgcagg ccgaagccga tggtcacgcc gtcgaaagca ccgtcggcga agggcagacg
     3541 caaggcgtct ccctcgacga agggcacgcg cctgccgtca gtgccggcct tgcgcgcggc
     3601 cagctcgagc atcggccggc agaagtccac gcccacgacg cgcgcgccgg gtgcggcctc
     3661 cagcacggcg agggcgaggt cgccagtccc gcaggcaatg tcgaggacac gcgcgtccgg
     3721 cgcgagcgcg gcgcagagtc gcttcgccac ctgtcggcgc cagcgccggt cggtgtttcc
     3781 cgacagcagg tggtttaaaa gatcgtagcg gccggcgatg cgtgcgaaca tcccgcgcac
     3841 acgccgcgca tgttcgccct ccgagcctgt cacttgtagt tgctgtctgc tggctaacac
     3901 aaatatacct aaacgggagt cgtatggttg cggacgcttt gcgtttattt tgtgattcgg
     3961 ctacggccga cgctgcgtgg gcgacttgaa cggggaggct tgcgccggct tcggggttgg
     4021 tttagccgtc gcgtcgccgc ttgtttcaac ctcgcctaaa cgcgcgaggt cggcgcgcag
     4081 acgcgccgcg tcgccgacga caaccgacgc cgcctgaacg cggaagagtt tggacgcgac
     4141 gcgctgaacg tcggccgggg tgagcgagcc ggcggcgcgc gccatctccg acatggacgc
     4201 ctgggcagtg ccgtatgtct gtgagtcgag catgtcgtcg gcgagctgct ccccgctctc
     4261 ggcctgcttg ttgaaggcgg cgacgaccga cttcttcgcc gtctcaagct cgtcggccgg
     4321 gacggccgtc gcggccagcg actccataac ctttcgcgcc gcttcgagtg cctgcgccgc
     4381 ggcggaagtc gtcgggacgg aggcgccgag gaggaaggcg ccgtcgaggg cgcgcgcgtc
     4441 gtgtcggacg gtgacggccc ggcctttgag ttcgggcacg gcggcgagcc agcgcgagcg
     4501 cacgagttcg gccaggacgg cgccggtcgc cgcgtcgcgt tcggagcgag cgacgccgcg
     4561 caccgccacg cgcacctccg cgtccggcag gttggggaaa tcgacgacga gggggcgcgg
     4621 atcgaccgcc tcgggctggc ggaacgtcgg cggcacgatc tggtcgccct tgcgccacac
     4681 gccgaggaac tgtcggagcg cgcgcaacgc gcgccgccgc tcgaagccgc ccgagacgat
     4741 gagcgtcgcg ttgttcggga tcaggaaccg ctcgcgcgcc tggacgagat cgccgcgctc
     4801 aacgcgcgcc agagactcgg gcgtgcccgc gacgcgccgg ccgtaggggt acgacccgta
     4861 gagcctcgcc gagatcgcgt cgtcggccac ggcgccgggc gagacgccaa gctcgcgcac
     4921 gattttgacg cgcgcgtcgc gcacgcgccc gaagacctcg ggcgtgagct gcgtgttgac
     4981 gacggcgtta cgcaggagtt ccacgagccg ctcgaactgg tcggcgcggc ccgcgagcaa
     5041 cacgtcaatc gagtcgtggc cgaccgagac cgagagacgc ccttcgaggt cgcccgtcac
     5101 atactcgcgc gtctgcgggt cggggaagag cgcgtcgccg aggagcgcca tcaggccgtc
     5161 cttgccggcc aggtcgaagc tggcgccgtt gtgcaggcgt agacgcaaaa cggtctgcgc
     5221 ttcgcccggc cgctcgacga gcacgatcaa cagcccgttt aaaagctgct cgcgctgctc
     5281 cgcgcccgcc ggaggcgcag ggacggcctg cgcggcgcag ttggccggga gggcggcgag
     5341 aaaaaacagc agcacggcga ggggcccggc caacggtttt cgtgatgtgc gtgtctgagg
     5401 gttcataaac tgcgcacggg ccgcgcgctc ggcggcggcc ccgtccgtgt tcgataagct
     5461 gaacaaaaga tgattcttac ggaagcccgc gggcaatcgc gtttgccgga cgagtttaga
     5521 tgtcggccgt ggggccgtca actgtccggg atttgcgatt ctcgctcagg gagcagggct
     5581 taattgcgtg aaaccaaaaa cattttgacg cgcatcggag gacaaacttc cggctctttt
     5641 ccgcatccga atcaatcagg gtttgctggg cgtcaattgg cgaccctccg gccctcgaaa
     5701 gaaatggctc ctcgattgga catgccgccg gggagtttgg cactcgaaga acacctcgtt
     5761 ccgacacacg gcaggccctc tcgggcgatg ctcgcagcgg acggtctctg atataatgtg
     5821 catggctaga agcgggaggt agcgagagat gaaactttgc cgaacgtgtg gggaagagtt
     5881 cagtgacaaa tttgtattct gcccggtgga cgcttcggcg ctaaacgtcg ccgagaccgc
     5941 ccccgcccct gagccttcct ccccggccat acctgcatac gtcgccgacg agcgcacgac
     6001 cgtcgtcagc gccgctgccg tgaacgtcgc ctccgtcgag acccccgcgg ccgaccacgt
     6061 cgccgcttcg gcgtcaggca gcaacggcaa cacgccgtct ccgtccgcgt ccgtccctgc
     6121 ttcaatcgac cccgaggaaa cggccgtgtc gcaggcgttc gccccgcgcg gcgagtacca
     6181 cctgacgatg cttcaggacg agggcatcac gcgtcgtctg tcgaaggagc ttcgggagac
     6241 ggcgcacgag tacgagctga cgtggcccga gttcaagcgc gatccgggcg gcttcaccaa
     6301 gcgcatggcc accggctacg gccagatggg ccggcgcttc ttcaatcagc cctacgtccc
     6361 gatggccgtc atctcaggcg tcctcggcat ggcaatctta gtcaccctgc tcgtcttccc
     6421 cttcaacgcc gtctacaagt ggctcgcctc cgacggccgg ctcgtcgccg atgtcggcat
     6481 gacgctcgaa gaggtgcagc gccgctcgac gctcaagctg gcgccggccg cggcgcaggg
     6541 cgacggcagc acgctgacga ccgtgagcgg cgagaacctc ggcttcgact tcgagctggc
     6601 caacaagggc ctcaagttcg agtgggcgcg cgcttacctg ctgagtgtta acgaggagca
     6661 gaagatcaag gagatcaacg tcgagctgtc gcgcacgcca caggcgtgga gggagctggc
     6721 caccagcgtc cgcaacctcg acgagtcgct tgaggacagg ggctggagag tcttcaccga
     6781 aggcgagtcg aacactgaga acgcgaagtt cctcaaggcg agtctggccg agcagcagac
     6841 ggcgccgccc gcggagggcg accccggtga gctgcgttgg atcgaccgcg acgcgtcgaa
     6901 cgtcatcacc ttcaccgcaa agcgggccga gggcggtggc gaagcccgct tcgtttccta
     6961 cctcaaggtg gaagagctga cgcgcgagac gctcgtggcg atgctcgaaa tccccgagga
     7021 gcaggagaag cccgacgaag gcccggcggg ccgggccaaa ggcacgggcg gcggcatgaa
     7081 gcccaagcag gagaagccgg gcggcggcgg cggcggcgga cgcaaagaag ccctgccggc
     7141 ctcctttggc aaagtgcctc cgggcgcgtt gcagccgcag atcgtcgcca tcacgccgca
     7201 cccgccggcc atcaagaacc cgagcctgcc ggtgacgccg cacatcattg ccgacccgac
     7261 gctcttcccg cccgacaacc ggccgattcc ttacggcgac ccgaagtcaa cctcgaccac
     7321 gccgagcgcg gggccgggtg agggcgccgg catcggcgac ggcaagggca cgggcgtcgg
     7381 ccccggcgag ggcgccggct tcggaccggg ccgcggctac aacaccggcg gcggtgatgg
     7441 gaaaatcggc ggcggcggcc ccggcggcgg cggcggcggc accgactaca acaagacctt
     7501 caacgccaag gacgtgacac gcaaggccgt cctgaactcg aagccggaac cgggcttcac
     7561 cgaggaagcg cgcaagaaca atgtcaccgg cgtcgtcaaa ctgcggctcg tcctcggcgc
     7621 cggcggctcg gtgtcgaaca tctcggtcgt caagggtctg cccgacggtc tgacggagcg
     7681 ggcgattgcg gcggcccgcc ggatttcctt cacgcccgcg cagaaggacg gccgccccgt
     7741 ctcgcaatgg gtgaccatcg agtacaactt caacatctac taaggcgggc cgcgtaaagc
     7801 tcgcgaagct cactcaagtc tgtgaacaag ggccatctgg ttaaagcaac cgggtggccc
     7861 ttcttatttc ccgcgacggc gtctggtcgg aacactgtcg catcccgcgt cgctacagta
     7921 gaatctgtgc tccaatgctg agaagggtgt tcctcaatac atatctccgg gccgaggccg
     7981 aggcgcggga gcagagacta ccggggcgat ttcaaggcgt ggacttgaag gtcatctcgg
     8041 tctgcatcat cgtcgccctc tcgctgacga tgattaacta cgtcggcgaa ctcgtctacg
     8101 tgctcgacgc actggacagg ctcgggccgg ccggcgcgga cgagcgtcta tggagcggcc
     8161 tcacgaactt ctccaacgaa aggctcgcgg ggccgatctg gtgggcgtgc gtcactgtgg
     8221 ttttctactt cgtcgttccc gcgttgttca tccggcttgc gctaaaggag ccgctgtccg
     8281 actacggact caaactgcgg ggcgcgttca aggactacgg cctctatctt ctgatgttcg
     8341 tcgtcatgtt tcccgtggtg ttactcgtct caggcgcggc gggttttcag gccaagtatc
     8401 ccttttatca aatggcgcgg ggcgagtcgc tgttgcctta cttctggcag tgggaagtct
     8461 tctacttcct acagttcttc gcgctggagt tcttcttccg cggctttatg ctgcacggcc
     8521 tcaaacatcg cttcggctat tacgcggtct tcgtgatgac catgccctac tgcatgatcc
     8581 acttccgcaa gcccttcctc gaagcactgg cggcgatcat cgcgggcgtg atactcggca
     8641 cgctgagcct caagagccgt tcgattttgc ccggcgtggc catccactac agcatcgcca
     8701 tcgtcatgga cttggccgcg ctctggcgta aaaatctgct gtggtgacga ccgcccgcgt
     8761 agccgggcga cccggtcggc ggccccgcag gtgaggagct tcttcgatgg ccttctgggc
     8821 gcgcgccaaa gtcgcagtca gaaaaaccga cctcgccgcc agcggtctgt tcgtccaatc
     8881 cttcaacgtt gtcaaagagg ccgccaaccg agtgttgggc ctgccagacc tcgccggtca
     8941 ggttttagga ctggccccgg tcaagacgct gcggctgcgc gtcgtcattc tgagggatga
     9001 gcagtcgcgg tcgctggccg aagcgcgcga ggtgatgccc tccgtcgaag cggcgcgggc
     9061 aatcttcgag cgacaggccc gaatcaaggt gtcgcccgcg cggggcagtt tcatccagac
     9121 cctcgacacg gccgctcccg ccgacgctct cgacgtacat tgcgacttcc acgcgtggaa
     9181 ggaaggactg cgcgaggccg gcaacttctt cgagacttat ctcggacaac acgcggccgt
     9241 catgctgacc ggctacgccg cccccatcac cgtcttcatc gtgcgcgacg taagcggtaa
     9301 aggcggttgc tcactcgggc ctctgacgga ctacgtgacc gtggccatct gcgggctaga
     9361 ctcttgcggg gaagacgagg aaggccgcgc cgtggcgcgt ctgctcgcgc acgaagtcgg
     9421 ccacgcctgc gggttgtggc acgtccgcga cccgtcgaac ctgatgcacc cacgcggccc
     9481 cggcgtcaaa ctccgcccgt ggcagagggc gatacttcgc aactcgcgac acgtcaccta
     9541 cctttgagca acgcccacgg cttcacgaaa acagttgacc gcagatgacc gcgcaggccc
     9601 gccggtatat actctctccg tcattccaag cccactcggg cgagtagctt cagtgtccga
     9661 aactcccaca cgcgtcgaga acgtcgtctg cgtcgccagc tttttcaagg gcgcggattt
     9721 cctgcgcgag tgtcgcgcgc gcggcgcgcg cgtcttcctc gtgacgcggg agcggacgct
     9781 gggtgaggag tggccgcgcg aggccatcga cgacctcgtc gccatcccga acgacgccag
     9841 cctcgacctc ttcctctacg ccgtcagcca tctcgcgcgc cgcgtgagaa tcgaccgcgt
     9901 cgtcgcgctc gaagagttcg atgtcgtcaa cacggcgctc ctgcgcgagc aactgcaagt
     9961 ccccggcatg cacggcacgg cggcgcgcgc cttccgcgac aagctgacca tgcgcgttcg
    10021 cgctcgcgag gccggcatcc gcgtccccga cttcgtccac gtcctcaacc acgaagcgct
    10081 ccacgccttc atgacgcgcg tgagcgcgcc gtgggtgctc aagccgcgct cggacgtgtc
    10141 ttccatcgga atcaagaagc tccacgaccc ggagcaggtc tggcgagcca tcgacgaact
    10201 cgacgcgcgc ggcgcgctgc acgagcgctc gccctactac gtgctggagc aatacgtggc
    10261 gggcgaggtc ttccacgtcg attcgctggt cgaggacggc gaggtaacgt tcgcgggcgt
    10321 caaccgctac gggcggcctc cgctcgacgt ggcccaacag ggcggcgtct acatctctca
    10381 cacgctcgac tacgactcgg aagaccgcgc gcgtctcgaa gagttcaacc ggcgactgct
    10441 caaaagcctc aggctggaga gcggggccgc gcatgccgag ttcatcaagg gcgaggccga
    10501 cggcgagttc tacttcctgg aagtcgcgtc gcgcgtcggc ggcgcctaca tggccgagac
    10561 gctggaggcg gcgagcggcg tcaatctctg gcgcgagtgg gcgcgtctgg aactggcggg
    10621 cggctcgcgt gcggccgaag agcgcccgca gccgcgacgc gaatacggcg gcatcgcgct
    10681 ttcactcgcg cgtcaggagt ggcccgacac gtctcaatac acggacgcag aaattgtcgg
    10741 gcgcgtgcgc aaggcttacc acgtcggcct cgtcgtcggc tcgccgagcc tcgcgcgcgt
    10801 cgaggaactg ctcgacgact acgcgcgccg cttcgccgcc gacttctgcg ccgtcgcgcc
    10861 gccgcacgag cgcccgccgc tctgagaatt tcaaatcaca ctggggaaat tacacgcatg
    10921 agtcaaatca aagtcgcgcc ctacggctcc tgggagtcgc cgataacgtc ggagctgatc
    10981 gtctcggcca cggtgggtct gggccagtgc ctcattgatg gcgatgactt gtactggagc
    11041 gagatgaggc cgctcgaagg cgggcgcagc gtcgttgtgc ggcgaacgcc cgacggccgg
    11101 acggccgacg tgacgcccgc gggcttcaac gcgcgcacgc gcgtccacga gtacggcggc
    11161 ggcgactaca cggtgcgcgc cggcatagtt tacttctcga acttcgccga ccagcgactc
    11221 taccgtgtga gcgcgggcga acagcctgct ccgctcacgc ccgccggcga ttaccgctac
    11281 gccgattgcc tgctcgactc gcgccgcggg cttctctacg ccatccgcga agaccattca
    11341 gacaagacgc gcgaggccgt caactgtgtg gtgtgcgtgc gcgccgacgg cgacgaatcg
    11401 ggcgggcgtg tgctggtttc gggcaacgac ttctactcgt caccgcgcct cagccccgac
    11461 ggccgccggc tcgcatggct cacctggaac catccgaaca tgccgtggga cggaagcgaa
    11521 ctgtgggtcg gcgaattgga cgacgatgga ttggtgcgtt cgtcgaggaa ggtcgccggg
    11581 ggcgtcgatg aatctatctt ccagcccgag tggtcgcccg acggcacgct ctatttcagc
    11641 tccgaccgga cgggttggtg gaatctctac cgcgagacgg tcggtgaggg cgtcgaaccg
    11701 atttgcggaa gggaatcgga gttcggccag ccgcagtggg gcttcggaat gtcgatctac
    11761 gccttcgccg acagcgggcg tatcgtcgcc gcccgcgtcg agcgcggcac cgcgcgcctg
    11821 tgcgtcatcg acacggcgac caaagaacta cgcgacgcgg agactcctta caccgacatc
    11881 acctacgtgc gcgcgtccga cgggcgcgtg gtcttccgcg gcgcttccgc aacggagcgc
    11941 gctgggatcg tcgcgcttga cctcgacacg ggacgcaccg atgtgttgcg acgttccagc
    12001 gagcaggtga tcgacgcggg ctacgtctcc gcggcgagcg ccgtcgagtt tccgaccgag
    12061 ggcgggttga cggcgcacgc tttctactac ccgccgcgca accgcgacta cgccgcgccc
    12121 gatggcgagc gcccgccgct cctcgtcaag tgccacggcg ggccgaccgc cgcgaccgtt
    12181 ccctcgcttc gtctggagac gcagtactgg acgagccgcg gcttcgccgt gctcgacgtg
    12241 aactacggcg gcagcagcgg ctacgggcgc gagtaccgcc gccgcctcaa cggcgagtgg
    12301 gggattgttg acgtagacga ctgcgtgaac ggcgcgcgcc acctcgtcgg gcgcggcgag
    12361 gccgacggcg agcgttgcgc catcacgggc ggcagcgcgg gcggcttcac ggtcttgaac
    12421 gcgctcacct tccgcgaaca gttccgcgcc ggcgcgagcc actacggcgt gagcgacctc
    12481 aaggggctga tcgaggacac gcacaagttc gaggcgcgtt acctcgaccg gctgatcggg
    12541 ccgtacccgg agcgcgcaga cctctaccgc gaacgctcgt cgatcaactt cacggagcgt
    12601 ctgtcgtgcc cggtcatctt ctttcaggga ctcgaagaca aagtggttcc gcccaaccag
    12661 gcggagttga tggtggaggc tttgaggaag aagggcctgc cggtggccta cgtcccgttc
    12721 gagggcgagc agcacggctt ccgcatggcc gagaacatcc ggcgggcgct cgacggcgag
    12781 ttctacttct actcacgcgt cttcggcttc gagccagccg agcgcatcga gccggtgttg
    12841 attgagaatt tatagtttag acatctgttg aatgcctgga tgataggtga ggtggcggaa
    12901 gctcgttact tgcgtgtggc gtaaggcgat tcaccgcaga ggcgcagagg gcgcagagtt
    12961 cacgccgagg gttgagcctt acgatctacg tcgagtgtgt cgctcaagcc tgcggcgccc
    13021 cctctgcgtc ctctgcgcct ctgcggtgag tcctactctc ctcacactca acaaaaagca
    13081 tcactcaaaa aggaacagaa aagggcgcgg gcgcgggtcg attgatttga cccgcgcccg
    13141 cgctcttgtt tattgtgagc ggcgtttagc tacgccggaa gccgcgctgc ttgcgcttgt
    13201 tgcgcttgcg cgccatcgcc gac
//
LOCUS       DASVES010000502         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_736392, whole genome shotgun sequence.
ACCESSION   DASVES010000502 DASVES010000000
VERSION     DASVES010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736392"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF240_21165"
     CDS             complement(<1..206)
                     /locus_tag="VF240_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177784.1"
                     /translation="MDLAEQLIERIADPYLTHCERSHLRCQLAKALAESGDYEGARKA
                     LGELWSRVGERPRLDELDQATAAEV"
     gene            348..641
                     /locus_tag="VF240_21170"
     CDS             348..641
                     /locus_tag="VF240_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177785.1"
                     /translation="MALATHNFTFLENIMKSLQRPCAAIAMTLILTMTAFAGDISTGL
                     VTAPPPPPPQQSSAMTGGMETPLNTDEASNEAASLSHGAEIGLSLLQSVLALL"
     gene            complement(919..2247)
                     /locus_tag="VF240_21175"
     CDS             complement(919..2247)
                     /locus_tag="VF240_21175"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7177786.1"
                     /translation="MDHDTDERPEGPPVVGAEELAAVEEKTRIVLRMASRKLLRIVLV
                     AIVLFFLIKGALWVLYALTGVVLLVVLSVFFAYLIAPLVEMVRRPFKVRGREREMPRV
                     VAIAIVYVALFGSTFLAFQILSPRLSTQLEQLVQKAPEYLENSRARASRLNEMLSQLN
                     LPPAVRQWTYKQVTTSLDDAGEYVKGQGFQSAVTFLVYVPWLVLIPILAFFLLKDADS
                     FRRSALQMIPQGRLRWRGDEFFQDVNSTLAAYIRAQLIACLLIGTVCTIGFYIIGVQY
                     ALVLGVVAGFLEFIPLLGPLVVAVLAVIVASFYSVNQIIGVLLFLGVLRIIHDYYTYP
                     LIIGQGVHLHPLAVILAILAGHEMAGVTGIFLAIPVIAILTVTHRHWLEHRGGQGIVA
                     DILKPAEQAVGATQEGDHNQLNAPAQADQPAPAPTSPHAPATTQPAPTDA"
     gene            complement(2510..2896)
                     /locus_tag="VF240_21180"
     CDS             complement(2510..2896)
                     /locus_tag="VF240_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177787.1"
                     /translation="MPANFATEQRSGPRYLVHIAVRAEWDDDGSGEHFSVVGETENVG
                     PAGALVHLETLPQVGSRVQLIVLDAQGTELRTVTEVIRLERNPVQPLAALLLIDATDE
                     WRGLVWEPSAALESKSDEDYESEYEH"
     gene            complement(3075..>3280)
                     /locus_tag="VF240_21185"
     CDS             complement(3075..>3280)
                     /locus_tag="VF240_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177788.1"
                     /translation="RRHVAHEIGALARPDEIRFSDQLPKTRSGKIMRRLLREIASGGA
                     VAGDTTTLEDFSVLEKLRAQEEE"
BASE COUNT          614 a          952 c         1100 g          614 t
ORIGIN      
        1 acttccgccg ccgtcgcctg gtctagctca tctagtcggg ggcgttcgcc gacgcgcgac
       61 cacaattcgc ccagcgcctt acgggcgcct tcgtaatcgc ccgactccgc tagagctttt
      121 gcaagctggc aacgcagatg cgaccgttcg caatgagtaa ggtatgggtc ggcaatccgt
      181 tcgattagct gttccgctaa atccatgtga ggctttccct tctttgcgat tgacaataaa
      241 gttgcatcgc gtaaagcgtg ggtgatggtt ccctcgttgg ggttgtctgg cggatgttaa
      301 gatgcgcccg cgaaactcct cctgggtggc tgttctggcc gtgattgttg gcacttgcca
      361 cccataactt cacgtttctg gagaacatta tgaaaagctt gcaacgtccc tgcgcggcta
      421 tcgcgatgac tctcattctc acgatgaccg cttttgccgg tgatatttca accggactag
      481 tgactgcgcc accaccaccc ccgccacagc agtcttcggc aatgaccggt gggatggaga
      541 cgccgctcaa tacggatgag gcgtcgaatg aggcggcatc gctgagtcac ggcgcggaaa
      601 tcgggttgag cttgctgcaa agcgttctgg cgctgttgta gcggctcggt gtaattgaaa
      661 tatttcgggc tgcgacgggt cgggtcgtcg cggccgaata tacgtgtgct tgggagagcg
      721 ccgtagagcc gaggtgtgcc cggcgaggag gcactcacag aaggcgtgag attacgcctg
      781 cgcggcgcgc aaagcaataa ccttctgcgt tttcgggtaa gtgccggccg ccgcgagttt
      841 tactgtctgt tgcaataaaa cgcgcaagtg ccggaagacg cgatacgggc cggcagtgac
      901 aggcccgcgc cgtgaatgtc aggcgtcggt cggggcgggc tgggtggttg cgggggcatg
      961 tggcgatgtc ggagcgggcg cgggctggtc ggcctgtgcc ggcgcgttca gttgattgtg
     1021 gtcgccctct tgagttgcgc ccacggcctg ctctgccggc ttgaggatgt cggcgacgat
     1081 tccctgtccg ccgcggtgtt cgagccagtg gcggtgtgtg acggtgagga tggcgatgac
     1141 gggaatggcg aggaagatgc cggtcacgcc ggccatctcg tgaccggcga ggatggcgag
     1201 gatgacggcg agcgggtgca ggtgtacgcc ctgcccgatg atgagcgggt aggtgtagta
     1261 gtcgtggatg atgcgtagca cgccgaggaa gagaagcacc ccgatgatct ggttgaccga
     1321 gtagaaactc gccacgatga ccgccagcac cgccaccacc agcggcccga gcagcgggat
     1381 gaactccaga aagccggcga cgacgccgag caccaaagcg tattgcacgc cgatgatgta
     1441 gaagccgatg gtgcagaccg tgccgatcaa aaggcaggcg atgagctgcg cgcggatgta
     1501 tgcggcgagc gtggagttta cgtcctggaa gaactcgtcg ccgcgccagc gcaggcggcc
     1561 ctgcgggatc atctggaggg ccgagcggcg gaagctgtcg gcgtccttga ggagaaagaa
     1621 ggcgaggata gggatgagga cgagccacgg cacgtagact aaaaacgtga cggcgctctg
     1681 aaagccctgc cccttaacgt actcgcccgc gtcgtcgaga ctggtcgtga cctgcttgta
     1741 cgtccactgg cgcacggccg gcggcaggtt gagttgcgag agcatctcgt tgaggcgtga
     1801 ggcgcgcgcg cgcgagttct ccagatactc gggggccttc tggacgagtt gctccaactg
     1861 cgtgctgagg cgcgggctga gtatctggaa ggcgaggaag gtcgagccga agagggcgac
     1921 gtagacgatg gcgatggcca ccacgcgcgg catctcgcgc tcacggcccc gcaccttgaa
     1981 gggccggcgc accatctcga ctaagggcgc gatgagatag gcgaagaaga ccgagagcac
     2041 cacgagcagc accacgcccg tcagcgcgta gagcacccac agcgcgccct tgatgaggaa
     2101 gaagaggacg atggcgacta agacgatgcg gagcagcttg cgcgaagcca tccgcaagac
     2161 gatgcgcgtc ttctcctcga ccgcggccag ctcctcggcc ccgaccaccg gcggtccctc
     2221 cggtcgctca tcggtgtcgt ggtccacaga agccaacgtc tcgtccctct cgccttcaag
     2281 cctcaagtct tcgcccctta attaaactga cggcgcgcgc cgaacgtaaa tcctgcccgc
     2341 aatgatgcac tactcggagg gggtttacaa agaagggatg aggggggaag gatgaaaggc
     2401 agtaagcagt aagcagtgag cagtaagcag tcaatgccgg ccgagattcc tgcgtcttga
     2461 ctgctcactg cttactgttc ttcctcatcc ttccgccctc atcccttact cagtgttcgt
     2521 actcgctctc gtagtcctcg tcggacttgg attcgagggc cgcggagggt tcccagacga
     2581 ggccgcgcca ctcgtcggtg gcgtcgatga ggaggagcgc ggccagcggc tggacggggt
     2641 tgcgctccag tcggatgacc tcggtgacgg tgcgtagctc cgtgccctgc gcgtcgagga
     2701 cgatgagctg cacgcggctg ccgacctgcg gcagtgtctc cagatggacg agcgcgccgg
     2761 ccgggccgac gttctccgtc tcgcccacga cgctgaagtg ctcgcccgag ccgtcgtcgt
     2821 cccactcggc gcgcacggca atgtggacga ggtagcgcgg cccggaccgc tgctctgtcg
     2881 caaagttggc gggcatccgt ttctcctccg aagaaaacaa gctacatggg gggtgtctaa
     2941 gataaacgac gaaagcccat tctgtcgaag aaagggagtc aggagtcagg agtcagaata
     3001 aaagcaagag cgccctcttc attcagcgcc ttcttcattc tgactcgcgg actcctgact
     3061 cctgactcct gacttcactc ctcttcctgc gcccgcagct tctccagcac ggagaagtct
     3121 tcgagcgtcg tggtgtcgcc ggcgacggcg ccgccggagg cgatctcgcg gagcaggcgg
     3181 cgcatgattt tgcccgagcg cgtcttgggc agttggtccg agaagcgtat ctcgtcgggg
     3241 cgggcgagcg cgccgatctc gtgggcgacg tggcggcgca
//
LOCUS       DASVES010000503        10491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_503928, whole genome shotgun sequence.
ACCESSION   DASVES010000503 DASVES010000000
VERSION     DASVES010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503928"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF240_21190"
     CDS             complement(<1..261)
                     /locus_tag="VF240_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177789.1"
                     /translation="MNAFDFIKTHWREVAALTAEHLLLVAVATALAVAIGLPVGVLLT
                     RRAALKKPVLAFAGVMQTVPSLALFGFLIPLPLLGIGARTATV"
     gene            complement(412..1185)
                     /locus_tag="VF240_21195"
     CDS             complement(412..1185)
                     /locus_tag="VF240_21195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7177790.1"
                     /translation="MSSELTEAVVEFRGVSYRVAPSGQIVIDGLDLEVRRGETLVLLG
                     ESGCGKTTTLRLINGLLLPTSGEVVVEGKTTSAWDKISLRRRAGYVIQDAGLFPHFTV
                     ERNVALVPSLEGWPEARVRERVRELLSLVGLDPEKFAERLPRELSGGQRQRVGVARAL
                     AADPPLLLLDEPFGALDPLTRAALQREFAALQRRLGKTAVFVTHDVREALTLGTRVGL
                     MRQGRLVVLDTPENFLRSEDPLARAYRETLQLPAMATAE"
     gene            complement(1360..2745)
                     /locus_tag="VF240_21200"
     CDS             complement(1360..2745)
                     /locus_tag="VF240_21200"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX7177791.1"
                     /translation="MSNEETTKRDDGSSSAFPLLTDPVSKESATLFAENLRRGAKPRS
                     EWVCGVEFELIGYDRAHDYARLDAAQVQRVLAGFAPSSNDLVYEGGVLVETGVGQMNR
                     LTVEPGGQIEFSGAPQRSLRDVERELRRFLARLHEIAEAESYAFLAVGFDPLRAIGEQ
                     HWFPKQRYDVMRPYLATRGERAWDMMTRTAAAQSNLDYNGPEDLAKKFLVANRLAPVV
                     TAMFANSPFEDGRPSGYKSTRAAVWLRTDPARSGLAPPALVDDFTPEAFVDYVLNVPM
                     IFVQREGRYLGAPRGMKFGDFLERGYGPFRPVFGDWADHLTTIFTDARLKQHIELRSA
                     DCNDLQMTVAFQALWQGLLYDPDALDEAFRLAPRLSHEDALALRESVARDGLAARSAG
                     VDVLGMARDVVRLAADGLQQSAPHEVAYLDVLRRQVVEEGVSPADVLLRQWHGAWAGS
                     TARLINHLRIA"
     gene            complement(2873..3898)
                     /gene="egtD"
                     /locus_tag="VF240_21205"
     CDS             complement(2873..3898)
                     /gene="egtD"
                     /locus_tag="VF240_21205"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX7177792.1"
                     /translation="MSSQPGDTREEEERLRLISLAPEGAHDDFAEDVRRGLTAGRKFL
                     LPKYFYDELGSRLFEAICLLPEYYLTRAEDEIFASHAEAIAAAASTGAARVSLLELGS
                     GSATKTRRIVEALVARQNDLLYVPVDISPAALEQSARVLLQSYPTLRVTAYAADYAAA
                     IPRLRESLEEDTRALVLFLGSNIGNFDPGGARDLLAALRRALRPGDRVLVGADLKKER
                     ATLEAAYDDALGVTAAFNLNILARINRELRADFDPRHFRHVARYDDRHGRVEMHLEST
                     RAQTVNIGALDLSVDFEEGERVHTENSYKSDLRDLLRLAEETGYASTNTWLDEQERFS
                     SNLFVAV"
     gene            4071..5423
                     /locus_tag="VF240_21210"
     CDS             4071..5423
                     /locus_tag="VF240_21210"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7177793.1"
                     /translation="MANDKTTIAREMREARAATLRLFDVAREEHLHCSPGFGFRPVVW
                     HLAHIGVFEGYWLLQKLQGLPPADERYERVFDPIATPREESKDLPTRREMEDYLARVR
                     SRALLALEEADLGGGGDNNTLTRDGYVFHLVIEHERQHQETLAYLLQLLDPSKKTRPT
                     IASESDADASAAQPLSSSSTRLATPGGEMVTIPAGDFPVGSIWDAFAYDNELPAHDRH
                     VPAFRLDRLPTTNAEYAEFVAEGGYRRREFWGEEGWSYREREGWEHPLFWTRAGGEGP
                     GWSERRMFEVGAIEPRHPVVGVSWYEAEAYARFRGKRLPTEAEWEKAAAWDGRSGRKR
                     RHAWGDAAPSDSLCNSDFRRWGTTPVGSFPAGASFYGCLDMTGNVWEWTADPFDAFPG
                     FEPYPYPEYSQAWFDGDHRVLKGGSWATGPTVLRTSFRNFFRRHFRIAFAGIRCAQDV
                     "
     gene            complement(5477..6958)
                     /locus_tag="VF240_21215"
     CDS             complement(5477..6958)
                     /locus_tag="VF240_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177794.1"
                     /translation="MPNARPSRRPHVALALSLASALFASAALALPRETAHAASTAAAA
                     CPVEPPPTPLRTLYRASERIAAARVGESVALEGKARQNMRRTTFVATESIKGDPGEKS
                     FFVYHPVWPSDANYVGNFRKGETLLLFLRRAEDVEPAGYYVTDIRHGAKRLSEADLKI
                     YFKRIEELQWVTRQQPLDRAELLEWLVRCVEEPATRWEGAFELAYSAQLAAYEAESAR
                     EKAHPVAAQIAALPLAVGGGKAEGEDANEDAGESVAGVSGGPDEGAEQAGDAVAAEAS
                     EESAAAAVNFVEDFSVSSEVEVFNGPDVDSKLIGELTAAQKERLAGALYSADKVGEGE
                     MQLVYVVENWDGARLTPFVVRHLQKLQDNPPYDAERLMHVLARVLKDDRLTRLAEMYT
                     EKAPYDDDSVETEEEAASAESEEADSTDAEASEAEGEEEAEEEAEQQADSGEAEEIMA
                     GALVTQAGGARPTASQVRGRMLHNFLAAVEKRMNAAAAETAQR"
     gene            7288..8532
                     /locus_tag="VF240_21220"
     CDS             7288..8532
                     /locus_tag="VF240_21220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177795.1"
                     /translation="MKTLYTFYADAAKIAVQAILAHKLRALLTLIGVIIGVASVVVVG
                     AAINGLNTYVRDRLTSVLGSNHFMIARMARTGEISEEEWEKMNRRNKRLELEDYEWVK
                     EHCTACSEVGAQFQNRVDLKHEGKELFGTSVLGSTPSMASIEDKTIADGRFIIPQEME
                     TAAMVCVVGYEIKEKLFDGGDPIGKTVSVRGLPLQIVGVEDKRGSMFGQSLDNNLYMP
                     LTTYRKIFGGRQSLQIHGKTETAEQFPAALEDARTAMRNKHRLSGDETDDFGLVNTAE
                     VGNAIDQFTGAIALVVVPITAISLIVGAIVVMNIMLVSVTERTFEIGLRKALGARRKQ
                     VLLQFLIESACLTMLGGVFGILLAAGVAWLISTLAQIPMTITLGYVVLSVGVSTLIGM
                     VAGIYPAFKASRLDPIVALARN"
     gene            8642..9898
                     /locus_tag="VF240_21225"
     CDS             8642..9898
                     /locus_tag="VF240_21225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7177796.1"
                     /translation="MKLKLPAFIPREILLMALDSIRAHKFRSFLTVLGIVIGVCVVIV
                     IASLITGLRNNIVQMIEEYGTNNIYAFHLSTGPQLHVDRAERARKPLNEADADAIRQH
                     AWAAEEVSNVLMVAWWDSTIQHKGQNYRRGNMSGVSANYARTANVSVREGRFISDIDD
                     QNRRNVMVIGVNVAESLFPGQPNVAGTEVKLGGKPFEIIGVLEKRKSAFFGENDEDNA
                     IYIPFRTARQVSPGSEWMMIIIRSKTGQLKTALDQTEEILRRQRGVKFNEANNFDLGT
                     ADKIVDQFDSIVGFIGIFAIAVSCVGLLVGGIGVMNIMLVSVTERTQEIGVRKAIGAR
                     RGDIIRQFLYEAMTLTFLGGVLGVIMAVVISKLVLFLLPQLPATVPLWAVATGLFVSI
                     AVGLIFGVWPAWKASRLDPIECLRYE"
     gene            9935..10420
                     /locus_tag="VF240_21230"
     CDS             9935..10420
                     /locus_tag="VF240_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177797.1"
                     /translation="MFCQSCGTALTKGLTYCNRCGARAAPSALTAVQKNDKLAEVVYV
                     LATFSAGVSLGGLAAVYFLIRELLRAGFEQSRIVVVTFFCLLAVVGLAFLLINQMSRA
                     LNVFLHSGAGADEGQATAANAPKLADVQTAQIEAPREPVASVTDHTTRTFDPVYEERR
                     S"
BASE COUNT         1734 a         3456 c         3497 g         1804 t
ORIGIN      
        1 gacggtggcg gtgcgcgcgc cgatgccgag cagcgggagc ggaatgagga agccgaagag
       61 ggcgaggctg gggacggtct gcatcacgcc cgcgaaggcg agcacgggtt tcttcaaggc
      121 ggcgcggcgc gtgagcagca cgccgacggg cagcccgatg gcgacggcga gcgcggtcgc
      181 cacggcgacg agcagcaggt gctccgccgt cagcgcggcc acctcccgcc agtgtgtctt
      241 gatgaagtcg aaggcgttca tcgcaggcca aagattgatt ggtcggctcg tcgggtcatc
      301 aactcaaaca cagtaccgcg agcggtagcg agcgggtgtg tctataacaa gagtgccgag
      361 tcgcgcatcc agacccgctc gctaccgctc gcggtactgt ttgatctccc ctcactccgc
      421 tgtcgccatc gccgggagtt gcagcgtctc gcggtaggcg cgcgcgagcg ggtcttcgga
      481 gcgcagaaag ttttccggcg tgtcgaggac gacgaggcgg ccctgccgca tcagcccgac
      541 gcgcgtgcct aaggtaagcg cctcgcgcac gtcgtgtgtg acgaagacgg ccgtcttgcc
      601 cagtcgccgc tgaagcgccg cgaactcgcg ttggagcgcg gcgcgcgtga gcgggtcgag
      661 cgcgccgaag ggttcgtcca gcagcagcag cggcgggtcg gcggccagcg cccgcgccac
      721 tcccacgcgc tggcgctgcc cgccggagag ttcgcgcggg agtcgttcgg cgaacttctc
      781 cgggtcgagt ccgacgagcg agaggagttc gcgcacgcgc tcgcgcacac gcgcctccgg
      841 ccacccttcg agcgacggga cgagcgcgac gttgcgctcc accgtgaagt gtgggaacag
      901 ccccgcgtcc tggatgacgt agccggcgcg gcggcgcaag cttatcttgt cccacgcgct
      961 cgtcgtcttg ccttccacca cgacttcgcc cgacgtgggc agcagcaggc cgttgatgag
     1021 cctgagcgtc gtcgtcttcc cgcaccccga ctcgccgagg agcacgagcg tctcgccgcg
     1081 tcgcacctca aggtcgaggc cgtcgatgac gatctgcccc gacggcgcga cccggtaaga
     1141 gacgcctcgg aactccacga ccgcttccgt tagctctgac gacatcgctg tcgattctac
     1201 cagtccgccg acgctccgcg cgcctttttg cgcctctcgc gtcctctgcg tcctctgcgt
     1261 cctttgcgta agacttccgg cgccgaacaa ataattttca cgcaaaggac gcagaggcgc
     1321 agaggacgcg agacaaaaat ttaagacgtg cgcgcggatt caggcgatgc ggaggtgatt
     1381 gatgaggcgc gcggtcgagc cggcccacgc gccgtgccac tggcgcagca gcacgtccgc
     1441 cggggagacg ccttcctcga cgacctgccg gcggagcacg tcgagatagg cgacctcgtg
     1501 tggcgcgctt tgttgcaagc cgtcggcggc cagacggaca acgtcgcggg ccatgccgag
     1561 cacgtccacg ccggccgagc gcgcggcgag gccgtcgcgc gcgaccgact cgcgcagggc
     1621 gagcgcgtct tcgtgcgaca gacgcggggc gaggcggaag gcttcgtcga gcgcgtcggg
     1681 gtcgtagagg agaccctgcc agagagcctg gaaggcgacg gtcatctgaa ggtcgttgca
     1741 gtcggccgag cgcagctcga tgtgctgctt gaggcgggcg tcggtgaaga tggtggtgag
     1801 gtggtcggcc cagtctccga agacggggcg gaaggggccg tagccgcgct ccagaaaatc
     1861 tccgaacttc attccccgtg gcgcgccgag gtagcggccc tcgcgctgca cgaaaatcat
     1921 cgggacgttc aggacgtaat cgacgaacgc ctcgggcgtg aagtcatcga cgagcgccgg
     1981 cggggcgagg cccgagcgcg cggggtcggt gcggagccag acggcggcgc gtgttgactt
     2041 gtagccggaa gggcggccgt cctcgaacgg cgagttggcg aacatggccg tcaccacggg
     2101 cgcgaggcgg ttggcgacga ggaacttttt ggcgaggtct tccgggccgt tgtagtcgag
     2161 gttcgactgg gcggcggccg tgcgcgtcat catgtcccac gcgcgctcgc cccgcgtggc
     2221 gaggtagggc cgcatcacgt cgtagcgctg cttcgggaac cagtgttgct cgccgatggc
     2281 gcgtaagggg tcgaagccga cggcgaggaa ggcgtaggat tcggcctcgg cgatctcgtg
     2341 caggcgcgcg aggaagcggc gcagctcgcg ctccacgtcg cggaggctgc gctgcggcgc
     2401 gccggagaac tcgatctgcc cgcccggctc gaccgtcaga cggttcatct gcccgacgcc
     2461 cgtctcgacg agcacgccgc cctcgtagac cagatcgttc gacgacgggg cgaagccggc
     2521 cagcacgcgc tgcacctgcg cggcgtcgag acgcgcgtaa tcgtgcgcgc ggtcgtagcc
     2581 gatcaactcg aactccaccc cgcacaccca ctcggagcgc ggcttcgccc cgcgccgcag
     2641 gttctccgcg aacagcgtcg ccgactcctt cgacaccggg tcagtcagca gcgggaaagc
     2701 gctgctgctg ccgtcgtccc gttttgtcgt ttcttcgtta gacattcgcg gaaataatta
     2761 acatggatgg acaggataag caggatggga tgatttgaaa tctgaaatct cgaatctgaa
     2821 atcagtcttt ctccctcctg cttatcctgt ccatccatgt aaattttatc tttcagacgg
     2881 cgacgaagag gttgctgctg aaacgctcct gctcgtcgag ccaggtgttg gtgctggcgt
     2941 agccggtctc ttcggcgagt cgcaggaggt ctcggaggtc gctcttgtaa gagttctcgg
     3001 tgtggacgcg ctcgccttcc tcgaaatcga cggacaagtc gagcgcgccg atgttgacgg
     3061 tctgcgcgcg cgtgctttcg aggtgcattt cgacgcgccc gtggcggtcg tcgtaacggg
     3121 cgacgtgtcg gaagtggcgc gggtcgaagt cggctctcag ctcgcggttg atgcgggcga
     3181 ggatgttcaa gttgaaggcg gcggtgacgc cgagtgcgtc gtcgtaggcg gcttcgagcg
     3241 tggcgcgctc cttcttgagg tcggcgccga cgagcacgcg gtcgccgggg cgaagcgcgc
     3301 ggcgcagggc ggcgaggagg tcgcgcgcgc cgccggggtc gaagttgccg atgttcgagc
     3361 cgaggaagag gacgagggcg cgcgtgtcct cttcgagact ttcgcgcagg cggggaatgg
     3421 ccgccgcgta gtcggcagcg taagccgtga cgcgaagcgt ggggtacgac tggagcagca
     3481 cgcgcgcgct ctgctccagc gccgcgggcg agatgtccac ggggacgtag agcagatcat
     3541 tctggcgcgc gacgagcgcc tcgacgatgc ggcgcgtctt ggtggcgctg cccgaaccga
     3601 gttcgagcag ggagacgcgc gcggcgcccg tggaggccgc ggcggcgatt gcttcggcgt
     3661 ggcttgcgaa aatctcgtcc tcggcgcgcg tcaggtagta ttcgggcagc aggcagatgg
     3721 cctcgaagag acgggagccg agttcgtcgt aaaaatattt cgggaggaga aacttccgtc
     3781 ccgcggtaag gccgcggcgc acgtcctcgg cgaagtcgtc gtgcgcgccc tcgggcgcaa
     3841 gactgatgag gcgcaggcgc tcttcttcct cccgcgtgtc ccccggttgt gatgacatcc
     3901 gttctgcctt cattctttcg cgcacgccga ccgctcgccc gcgccccgtc cgtcccctga
     3961 agtacgcttg ctataatatc ccaacgcact ccattatcca cggcacgccc cttcgttcaa
     4021 cgcgggcgac gcacgcgggg aggaatgtga tcttgagtgc tgtgccggag atggctaacg
     4081 acaagacgac gattgcgcgg gagatgcgag aggcgcgcgc cgcgacgctg cggctgttcg
     4141 acgtggcgcg cgaggagcac ctgcactgta gccccggctt cggcttccgc cccgtcgtct
     4201 ggcacctcgc gcacatcggc gtgttcgagg gctactggct gctccagaag ctgcaagggc
     4261 taccgcccgc cgacgagcgt tatgagcgcg tcttcgaccc catcgccacg ccgcgcgagg
     4321 agtcgaaaga tttgcccacg cgccgcgaga tggaggatta cctcgcgcgc gtgcgctcgc
     4381 gcgccctgct cgcgctcgaa gaggccgacc tcggcggcgg cggcgacaac aacacgctca
     4441 cccgcgacgg ctacgtcttc cacctcgtca tcgaacacga acgccagcat caggagacgc
     4501 tcgcctacct cctgcaactg ctcgacccgt cgaagaagac gcgacccacc atcgccagtg
     4561 agtcggatgc ggacgcgagc gcagcacagc ctttgtcgtc gtcatcgacg cgtctcgcaa
     4621 cacccggcgg tgagatggtc acgatccccg cgggcgactt ccccgtgggg tcgatctggg
     4681 acgcgttcgc ctacgacaac gagctgccgg cgcacgaccg acacgtcccc gcgttccgcc
     4741 tcgaccgcct gccgacgacg aacgccgagt acgccgagtt cgtcgccgag ggcggctacc
     4801 gccggcgcga gttctggggc gaagagggtt ggagttatcg tgagcgcgaa ggatgggagc
     4861 acccgctctt ctggacgcgc gctggcggcg aaggccctgg ctggagcgag cgccggatgt
     4921 tcgaggtggg ggcaatcgag ccgcgtcacc cggtcgtcgg cgtgagctgg tacgaggccg
     4981 aagcctacgc gcgcttccgc ggcaagcgtc tgcccacgga ggccgaatgg gagaaggccg
     5041 ccgcgtggga cggccgaagc gggcgaaagc gtcgccacgc gtggggcgac gccgcgccct
     5101 cggattcact ctgcaactcc gacttccgcc gctggggcac gacacccgtc gggagctttc
     5161 ccgccggcgc gagcttttac ggctgcctcg acatgaccgg aaacgtctgg gaatggacgg
     5221 ccgatccctt cgacgccttc cccggcttcg agccgtaccc ctacccggag tattcgcaag
     5281 cttggttcga cggcgaccac cgcgtcctca agggcggctc gtgggccacc ggccccacgg
     5341 tgctcagaac ttccttccgc aacttcttcc gccggcactt ccgcatcgcc ttcgccggca
     5401 tccgctgcgc gcaggacgtg tgaaatttaa agcggcagta gagggccacg gctttcgtcg
     5461 ggcggcggcc gtcgggttaa cgctgcgcgg tctccgcggc cgcggcgttc atccgtttct
     5521 cgacggcggc gaggaagttg tgcagcattc ggccgcgcac ctgcgaggcc gtgggccggg
     5581 cgcctccggc ctgcgtgacg agcgcgccgg ccatgatctc ttcggcctcc ccggagtcgg
     5641 cttgctgctc ggcctcctcc tccgcttcct cctcgccttc ggcctcgctc gcctctgcgt
     5701 ccgtcgaatc cgcttcctcc gattcggcag acgctgcttc ctcttcagtc tctactgagt
     5761 cgtcgtcgta gggagccttc tccgtgtaca tctcggcgag ccgcgtgagc ctgtcgtcct
     5821 tgaggacgcg ggccaacacg tgcatcaacc gctcggcgtc gtagggcggg ttgtcttgaa
     5881 gtttttgcag gtgacggacg acgaaggggg tgagacgcgc gccgtcccag ttctcgacga
     5941 cgtagacgag ctgcatctcg ccctcgccca ctttgtcggc gctgtagagg gcgcccgcca
     6001 gtcgctcctt ctgcgcggcg gtcagttccc cgatgagctt cgagtcaacg tccgggccgt
     6061 taaaaacttc cacctccgaa gagacgctga aatcctccac gaaattcacg gccgcggcgg
     6121 cagactcttc cgacgcctcc gctgcgacgg cgtcgcccgc ctgctccgcc ccttcgtccg
     6181 gcccgccgga caccccggcg acactctcgc cggcgtcctc gttcgcgtct tcgccttccg
     6241 ccttcccccc gccgaccgcc aacggcaagg cagcgatttg agcggctacg gggtgcgcct
     6301 tctcccgcgc cgactcggcc tcgtaggccg cgagctgggc gctgtaggcg agttcaaacg
     6361 cgccctccca acgcgtcgcc ggctcctcga cgcagcgcac tagccattcg agcagttcgg
     6421 cgcggtcaag cggctgctgg cgcgtgaccc actgaagctc ttcgatgcgc ttgaagtaga
     6481 ttttcaggtc ggcctcggac agtcgcttcg cgccgtggcg aatgtccgtg acgtagtagc
     6541 ccgccggctc cacgtcctcc gcgcggcgga ggaacagcag cagcgtctcg cccttgcgga
     6601 aatttccgac gtagttcgcg tcggacggcc acaccgggtg gtagacgaaa aaactcttct
     6661 cgccggggtc gcccttgatg ctctcggtcg cgacgaaggt cgtcctccgc atgttctgcc
     6721 gggcctttcc ctccagcgcg acggactcgc ccacgcgcgc ggccgcgatg cgctcgctcg
     6781 cgcggtagag cgtccgcagc ggcgtcggcg gcggctcgac ggggcaggcg gcggcggcag
     6841 tggaggcggc gtgcgcggtc tcgcgcggga gggcgagcgc ggccgaggcg aagagggcgg
     6901 acgctagaga cagcgcgagg gcaacgtgcg ggcgacggga cgggcgagcg tttggcatgg
     6961 gtgttctcct ttcgtttgct acccacgggc ctcggggaga cgctcgaatt tttggaacga
     7021 aatctatgcg cttagacaca gtctggcggg aaaaggttcc ttgggcggga ttgaaaattt
     7081 tccgcgtccg gcggcacgtc ctccgccgcc gcgcgactct ggcctcgcgg agtacgcgcg
     7141 ccggtctaaa tcagacgagg catcgataga ctgatgcggc cccttcgcgg aatgcggcgg
     7201 ggaggttttc gtaaacattg gcccggcgcc cgacgtatac ccaatgtctc gccgagacga
     7261 acgccgccca gaccgataag cgcctctatg aaaacgcttt acaccttcta cgccgacgcg
     7321 gcgaagatcg ccgtccaggc catcctcgca cacaagctgc gcgccctgtt gaccctcatc
     7381 ggcgtcatca tcggcgtcgc ctcggtcgtc gtcgtcggcg ccgccatcaa cggcctgaac
     7441 acatacgtcc gcgaccgtct cacgtctgtc ctcggctcca accacttcat gatcgcgcgc
     7501 atggcccgca cgggcgaaat aagcgaggag gagtgggaga agatgaaccg ccgcaacaag
     7561 cgtctggagc tggaagacta cgagtgggtc aaagagcact gcacggcctg ctccgaggtg
     7621 ggcgcgcagt tccagaaccg cgtcgatctc aagcacgagg gcaaggagct gttcggcacc
     7681 tccgttctgg gttcgacgcc cagcatggcc tccatcgagg acaagacgat tgccgacggc
     7741 cgcttcatca tcccgcagga gatggagacg gcggcgatgg tctgcgtggt cggctacgag
     7801 atcaaggaaa agcttttcga cgggggcgac cccatcggca agaccgtgag cgtgcggggc
     7861 ctgccgctcc agatcgtcgg cgtcgaagac aagcgcggct cgatgttcgg ccagtcgctc
     7921 gacaacaatc tctacatgcc gctgacgacc tatcggaaaa tcttcggcgg ccgccagagc
     7981 cttcagattc acggcaagac cgagacggcc gagcagttcc cggccgccct cgaggacgcg
     8041 cgcacggcga tgcgcaacaa gcatcgcctg tcgggcgacg agacggacga cttcggcctc
     8101 gtcaacacgg ccgaggtggg caacgccatc gaccagttca ccggcgccat cgcgctcgtc
     8161 gtcgtcccca tcacggccat ctcgctcatc gtcggggcca tcgtcgtgat gaacatcatg
     8221 ctggtgtccg tcacggagcg cactttcgag atcggcctgc gtaaggctct gggcgcgcgg
     8281 cgcaagcagg tgctcttgca gtttttgatc gagtcggcgt gcctgacgat gttgggcggg
     8341 gtgttcggca tcctgctggc ggccggcgtg gcgtggctca tctcgacgct cgcgcaaatc
     8401 ccgatgacca tcaccctcgg ctacgtcgtg ctctcggtcg gcgtctcgac gctcatcggc
     8461 atggtcgccg gcatctatcc ggccttcaag gcgtcgcggc tcgacccgat tgtggcgctg
     8521 gcgagaaact gataaagccg tgataagtga taagtgataa gtgataagaa aagacaaaag
     8581 acagatgctt tctcttatca cttatcactc atcactcatc acttatcatt gagttaaaga
     8641 aatgaagttg aagctacccg cattcatccc ccgcgagatt ctgctgatgg cgctcgacag
     8701 catccgggcg cacaagttcc gcagcttcct gacggtgctc ggcatcgtca tcggcgtgtg
     8761 cgtcgtgatc gtcatcgcct cgctgattac gggtctgcgc aacaacatcg tgcagatgat
     8821 cgaggagtat ggcaccaaca acatctacgc cttccacctc tcgacggggc cgcagctcca
     8881 cgttgaccgc gccgagcgcg cccgcaagcc cttaaacgag gccgacgccg acgccatccg
     8941 ccagcacgcg tgggccgccg aggaggtctc caacgtcctg atggtcgcgt ggtgggactc
     9001 gaccatccag cacaaggggc agaactaccg ccgcggcaac atgtcgggcg tctccgccaa
     9061 ctacgcgcgc accgccaacg tctccgtgcg cgaggggcgc ttcatctccg acatcgacga
     9121 ccagaaccgg cgcaacgtga tggtcatcgg cgtcaacgtc gccgaatccc tcttccccgg
     9181 acagcccaac gtcgccggca ccgaggtcaa actcggcggc aagcccttcg agatcatcgg
     9241 cgtgctggag aaacgcaaga gcgccttctt cggcgagaac gacgaagaca acgccatcta
     9301 catccccttc cgcacggccc ggcaggtctc gcccggcagc gagtggatga tgatcatcat
     9361 ccgctccaag acggggcaac tcaagacggc gctcgatcag acggaggaga ttctgcgccg
     9421 gcagcgcggc gtgaaattca acgaggcgaa caatttcgat ctggggacgg ccgacaagat
     9481 cgtcgatcag ttcgactcca tcgtcggctt catcggcatc ttcgccatcg ccgtctcctg
     9541 cgtcgggctg ctcgtcggcg gcatcggcgt gatgaacatc atgctggtgt ccgtgacgga
     9601 gcgcacacag gagatcggcg tgcgtaaggc catcggcgcg cggcgcggcg acatcatccg
     9661 gcagtttctt tacgaggcga tgacgctgac tttcctcggc ggcgtgctcg gcgtcatcat
     9721 ggccgtcgtc atcagcaagc tcgtcctctt cctgctgccg cagctccccg cgaccgtccc
     9781 gctgtgggcg gtggcgacgg ggctgttcgt ctccatcgcc gtcggcctca tcttcggcgt
     9841 gtggcccgct tggaaggctt cgcgcctcga cccgattgag tgcctgcgct acgagtgagc
     9901 cgaagcccga gggttcggtg tcaggagggg gaagatgttc tgccagtctt gcggcacggc
     9961 gctgacgaag gggctgacct actgcaaccg ctgcggtgcg cgcgccgccc cttcggcgct
    10021 cacggcggtg cagaagaacg acaagctggc cgaggtcgtc tacgtcctcg cgaccttctc
    10081 ggccggcgtc agcctcggcg gactcgccgc cgtctacttc ctcatcaggg agctgttgcg
    10141 ggcgggcttc gagcagagcc gtatcgtcgt cgtcaccttc ttctgtctgc tggcggtcgt
    10201 cggcttagcc ttcctgctca tcaatcagat gtcgcgcgcc ctcaatgtct ttctccactc
    10261 cggcgcgggc gcggatgaag gtcaagcgac ggccgccaac gcgcctaagc ttgccgacgt
    10321 gcagacggcg cagatcgaag cgccgcgcga accggtggcg agcgtcaccg accacacgac
    10381 gcggacgttc gaccccgtct acgaggaacg ccgcagttag caggcggccg gactatatca
    10441 gtgttcagtt gagtgcgcgg ggagtcgtac tcaccgcgga gacgcaaagg g
//
LOCUS       DASVES010000504         5034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1278393, whole genome shotgun sequence.
ACCESSION   DASVES010000504 DASVES010000000
VERSION     DASVES010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278393"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..614)
                     /locus_tag="VF240_21235"
     CDS             complement(102..614)
                     /locus_tag="VF240_21235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7177798.1"
                     /translation="MRQTLYLPWLEMQDEKESVRFNIRPAVSGEAAPLTELAVRSKSH
                     WGYDSQFIEDSRADLTVTPDAIDRGRVFVAESAGVVAGFYSLSGEGEEAEVELLFVEP
                     RQIGTGAGRALWRHAAATARRLGFRTLVVASDPFAEPFYCAMGAVRVGERESIVRAGR
                     MLPLLRYPLD"
     gene            complement(692..2008)
                     /locus_tag="VF240_21240"
     CDS             complement(692..2008)
                     /locus_tag="VF240_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177799.1"
                     /translation="MSKKKIGRAAAFLSLTLLIAGALLASGGARAGLAQGAKPAAAVN
                     PKSAAVQEATREVLRETSELRGLKVVREVASGAQSRPEIERMLIANLNESTTPEEMRS
                     SEVALKKFGLAPADFQLRPFIIAVLTEQIAGYYDPKRQTFYLADWVDLEGLQPVIAHE
                     LTHALQDQHFNLRMFENWPKHDSDAELAAQALVEGDATVLMMQYVMKNPQRALALFKS
                     MGNTSSEQLDRAPRALRETLMFPYEHGMTFAAQVHRRGGWPLVSQAYTELPKSTEQIL
                     HPEKYFTKEAPVADIAWRDVSAQLGKGWRVTDHDVNGEWGYYLILDEFLKSKSESLKA
                     AAGWGGDHYVLYEGPNKSDVLLAQYTHWDTEQDATEFFTAYVKRTERRYKHAGATQTP
                     AANSATRSAWTTAEGSVVIERRGQRVIVVEGLPKNYKPEVLDKLFS"
     gene            2114..2602
                     /locus_tag="VF240_21245"
     CDS             2114..2602
                     /locus_tag="VF240_21245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7177800.1"
                     /translation="MTDAVGADALFEYWHEATVRVRYAETDRMGVVYHANYLVWFEIG
                     RTEFCRARGFSYREMEENGRAFLVVAESYCRYKAPAFYDDELLIRTHITELRRRSVRF
                     GYELVRLSDGVTIAEGETGHVVTDQKGRVISMPDEYRTALTAPPIAVTRAEPVPEDIS
                     PE"
     gene            complement(2707..3492)
                     /locus_tag="VF240_21250"
     CDS             complement(2707..3492)
                     /locus_tag="VF240_21250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX7177801.1"
                     /translation="MRVLMIGDVVAQPGRVAVMERIQDIREQHSVDLAVMNAENVAGG
                     YSITPPLADGLFRAGIDVMTSGNHIFDKRDAVAYIDRQPRLLRPANYPPGTPGRGLWV
                     GEVRGVRVAVLNLMGRVFMPPTHDDPFRVADEQLATLDDSVRVRLLDIHAEATSEKSA
                     MGWHLDGRVSAVVGTHTHVQTADERILPQGTAYLTDIGMTGSYSGVIGMSKDDVIARF
                     RDPSQRRAEHSTGDVRICAALIDIDEETGRAREISRLSLTHES"
     gene            complement(3591..3775)
                     /gene="ssrS"
                     /locus_tag="VF240_21255"
     ncRNA           complement(3591..3775)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF240_21255"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3969..4334)
                     /locus_tag="VF240_21260"
     CDS             complement(3969..4334)
                     /locus_tag="VF240_21260"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7177802.1"
                     /translation="MSALKGSMDPAGTPTIRVEIYNQTYNIRSDGDSEYVTGLAEFVD
                     QRMREISSGTLTVDSLKVAILAALHIADELHRLKRLHEQADQQLAARSSECAEMLDRL
                     LKARTAVAEQPAGSKIAFT"
     gene            complement(4357..4644)
                     /locus_tag="VF240_21265"
     CDS             complement(4357..4644)
                     /locus_tag="VF240_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177803.1"
                     /translation="MMVGLTGLEKFSHLEDKIYRTIELTKTLRQEKETLERDVNSIRR
                     ELGGLVEEKGRLEVQVERLLVERDAIRAKVEAMLDALAVLDPEAAELVARR"
BASE COUNT          872 a         1569 c         1607 g          986 t
ORIGIN      
        1 tgaaaaatag cacatgagcg cgaggcaaga caatgcgctt ctgggcgggg tcggacaacg
       61 tgctcgaccg cgggccgttc agtcgaggcc gcgccacggg tttagtcgag cggatatcga
      121 agcaacggca gcattctccc ggctcggaca atcgactccc gctcgccgac gcgcactgcg
      181 cccatcgcgc agtagaaggg ttcggcgaag gggtcgctcg cgacgacgag ggtgcgaaag
      241 ccgagccgcc gggccgtcgc ggcggcgtga cgccagaggg cgcggcccgc tcccgttccg
      301 atctgacgtg gctcgacgaa gagcaattcc acctccgcct cttcgccctc gccgctcagg
      361 ctgtagaagc cggcgacgac gcccgccgac tcggcgacga agacgcgccc cctgtcgatt
      421 gcgtccggtg tgacggtcaa atcggcgcgg ctgtcctcga tgaattggga gtcgtagccc
      481 cagtgagact tggagcggac ggcgagttcc gtgagcggtg cagcctcacc ggatacggcc
      541 ggtcggatgt tgaagcgcac ggattctttt tcgtcctgca tttccaacca aggtagataa
      601 agggtctgac gcatttcgcg cctttgtgcc ttttacgtga agtcaaatct cacgaaaagg
      661 cgcaaaggcg cgaagaagag atggcggcga tttacgaaaa gagcttgtcg agaacttcgg
      721 gcttgtaatt cttcggcaaa ccctcgacga cgatcacgcg ctgcccgcgc cgctcgatga
      781 cgacgctgcc ttcggcggtc gtccacgcgg agcgcgtcgc ggagttggcg gctggggtct
      841 gcgtcgctcc ggcgtgtttg tagcggcgct cggtgcgctt gacgtaggcc gtgaagaact
      901 ccgtcgcgtc ctgttccgtg tcccagtggg tgtactgcgc gaggagcacg tccgatttgt
      961 tcggcccctc gtagagcacg taatggtcgc ccccccaccc ggccgccgcc ttcagcgatt
     1021 ccgacttcga tttcagaaac tcgtcgagga tgaggtaata accccactcg ccgttcacgt
     1081 cgtgatcggt gacgcgccag cccttgccga gttgcgccga cacgtcgcgc cacgcgatgt
     1141 cggcgacggg cgcctccttc gtgaaatact tctccgggtg gagaatctgc tcggtcgatt
     1201 tcggcagctc cgtatacgcc tgcgagacga gcggccagcc gccgcgccgg tgtacctgcg
     1261 cggcgaacgt catgccgtgc tcgtagggga acatcaaagt ctcgcgcagg gcgcgcggcg
     1321 cgcggtcgag ctgctcgctc gacgtgttgc ccatcgactt gaagagcgcg agggcgcgct
     1381 gcgggttttt catcacgtac tgcatcatca ggacggtggc gtcgccctcg acgagcgcct
     1441 gcgcggccaa ttcggcgtct gagtcgtgct tgggccagtt ctcgaacatg cggaggttga
     1501 agtgctgatc ctgtagggcg tgcgtcagct cgtgggcgat gacgggctgg agtccttcga
     1561 ggtcaaccca gtcggcgagg tagaacgtct gccgcttggg gtcgtagtag ccggcgatct
     1621 gctcggtcag gacggcgatg atgaagggcc ggagctggaa gtcggccggg gcgaggccga
     1681 actttttgag ggccacctcg gaggagcgca tctcctcggg cgtggtgctc tcattgaggt
     1741 tggcgatgag catccgctca atttcgggcc gcgactgcgc gccgctcgcc acctcgcgca
     1801 ccactttcag cccgcgcagt tcgctcgtct cgcgcaaaac ttcacgggtc gcctcctgca
     1861 cggccgcgct cttcgggttg accgccgccg ccggcttcgc gccctgcgcc agccccgcgc
     1921 gcgcgccgcc cgacgcgagc aaggccccgg caatcaagag ggtgagcgac aggaaggccg
     1981 cggcgcgccc aatctttttc tttgacatcc tgaacctttc gcttttatag tccgctaact
     2041 cgctcttaga cgaatctctc tttcggcccc gctacggaaa gagttttcac gaagcaggag
     2101 gcgttcgctt tcgatgactg acgcggtcgg tgcagatgcc ctgttcgagt actggcacga
     2161 ggcgacggtg cgcgtgcgct atgccgagac cgaccggatg ggcgtcgtct atcatgccaa
     2221 ctacctcgtc tggttcgaga tcggccgcac cgagttctgc cgcgcccgcg gcttctccta
     2281 tcgcgagatg gaggagaacg gccgcgcctt cctcgtcgtc gccgaatcct actgccgcta
     2341 caaggccccc gccttctacg acgacgaact cctcatccgc acgcacatca cggaactgcg
     2401 ccgccgctcg gtccgcttcg gctacgaact cgtccgcctc tccgacggcg tgaccatcgc
     2461 cgagggcgag accggccacg tcgtcaccga ccagaagggc cgcgtcatct ccatgcccga
     2521 cgaataccgc accgcgctca ccgccccacc cattgccgtc actcgcgccg agccagtccc
     2581 cgaggacatt tctcctgaat agtgataagt gatgagtgat aagtgataag agctattagc
     2641 tgatagccgt tagctaacag caaggcttct ttcttatcac ttatcactca tcacttatca
     2701 ccgtccttaa gactcgtgtg tcaaagagag gcgcgagatt tcgcgtgcgc ggccggtctc
     2761 ttcgtcgatg tcgatgaggg cggcgcagat tcgcacgtcg cccgtggagt gttcggcgcg
     2821 gcgttgcgag gggtcgcgga agcgggcgat tacgtcgtcc ttgctcatgc cgatgacgcc
     2881 cgagtagctg ccggtcatgc cgatgtcggt caggtaggcc gtgccctgcg gcagtatccg
     2941 ctcgtcggcc gtctgcacgt gcgtgtgcgt gccgaccacg gccgagacgc gcccgtcgag
     3001 gtgccagccc atcgccgact tctcggacgt ggcctcggcg tggatgtcta agagtcgcac
     3061 gcggacactg tcgtcgagcg tcgccagttg ttcgtcggcg acgcggaacg ggtcgtcgtg
     3121 cgtcggcggc atgaagacgc ggcccatgag gttgaggacg gcgacgcgga cgccgcgcac
     3181 ctcgccgacc cagagaccgc ggcccggtgt gcccggcggg tagttggcgg ggcgcagcag
     3241 gcgcggctgg cggtcgatgt aggcgacggc gtcgcgcttg tcgaagatgt ggttgccgga
     3301 ggtcatcacg tcgatgccgg cgcggaagag accgtcggcg aggggcggcg tgatggaata
     3361 gccgcccgcc acgttctcgg cgttcatcac ggcgaggtct accgaatgct gctcacggat
     3421 gtcctgaatg cgctccatca ccgccacccg gccgggttgc gctaccacgt cgccgatcat
     3481 caggactctc ataaaagcaa aagccgtaaa tcgtcaatcg taaatcgtga atcgataagt
     3541 caattctcta ttcacgattt acgattgacg atttacggca attggcaggg ggccgctccg
     3601 cgttggccgc tccgaagccg taattgaacc tgcacaacag gtgggtgacc tttaagagcc
     3661 gcagcctctg gcactcccta tcaatgaggg actgcactgg ctggcggcgt cggtctccct
     3721 ttcaaacggc gttggctcaa tcatcgtaaa tcggtgacga accactcaga atcgaaccct
     3781 ctgccacgag aatactagca taaacgcggg cggaatgtga gaggtgcttt cggttttttt
     3841 tcgccctccg cgcccactgt gaacgccccc cgcgcggcgc gcatttacat cagtttcgac
     3901 aaagccgggg caagtttcgt gtgggacggg cggtgcgctt tgcgaggtgt cggcggcaaa
     3961 ggcccgtctc aggtgaaggc gatcttgctg ccggccggct gttcggcgac ggcggtgcgg
     4021 gccttcagga gacggtcgag catctcggcg cactcggagc tgcgcgcggc gagctgctgg
     4081 tcggcctgct cgtggagacg cttgaggcgg tggagttcgt cggcgatgtg gagggcggcg
     4141 aggatggcga ccttgaggga gtctacggtg agcgtgcccg aagagatttc gcgcatccgc
     4201 tgatcgacga actcggcgag gcccgtgacg tactcgctat cgccgtcgga gcggatattg
     4261 taggtctggt tgtagatttc gacgcggatg gtcggcgtgc cggcggggtc catcgaacct
     4321 ttgagtgcgc tcatcacgtt acgcctcggc gtgagattac ctgcgcgcca ccagttcggc
     4381 cgcctcgggg tcgagcacgg cgagggcgtc gagcatcgcc tcgaccttcg cgcggatcgc
     4441 gtcgcgctcg acgagcaacc gctcgacctg aacttcgagc cgccccttct cttcgaccag
     4501 cccgcccagc tcgcgcctga tcgagttgac gtcgcgctcc agagtctctt tctcctggcg
     4561 gagcgtcttc gtcagttcga tggtgcggta gattttgtct tccaggtggg aaaacttctc
     4621 gagtccggtc agtccgacca tcatctcacc tcaaaaacga gtcctcaaaa acgaaccccg
     4681 attatgcgac ttcgcagccc tgagcgcaag agagaaaagt tgtcgtcggt ggtcagtagt
     4741 cagtagtcag tggtttgagc gtcgagacgg tggaattaaa cacttaatcg cgcccgtctt
     4801 ccctgtttac caagacgact gatgaatgac gagtgtcttc tgacaacgga ctactgacca
     4861 cggacgacgg acagcttttc aaactctcat ctgcgccccg aatgtttctt ctaaggctga
     4921 gacgacgcgc gcgtgcgagg cgtcaacctc atcgtcacgc agtgtgcgtt cgtccgaacg
     4981 atactcgacg cgcaacgtca acgagcgctt cccttcgggc agaccggcgc cctc
//
LOCUS       DASVES010000505        25047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_830218, whole genome shotgun sequence.
ACCESSION   DASVES010000505 DASVES010000000
VERSION     DASVES010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 25047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25047
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830218"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VF240_21270"
     CDS             complement(<1..1346)
                     /locus_tag="VF240_21270"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX7177804.1"
                     /translation="MTNKETDAKALKVAKELKSAHNWKHLARWHGSGYYELQTDDTGD
                     VPVRMFLTPQLLADAEDILYRQIINATRFPGVRLVVLTPDVHYGYGVPVGCVILTDAE
                     SGAVAMGPVGFDIGCFRADTLVPTADGHSYPIGALAESGEEIHVYVLSETRKIVVAKA
                     TARRTRTDAPLVKVTLDNGREIFCTPDHEFMLRDGDYRQAQELQPGASLMPFDMRADK
                     DGYTMVRHPATGTNQRVHWVMARQGLLGDIPAFAGQQTIIHHRNFLPSDNRPENLQFM
                     GHLDHLSYHHSIAERNPHFRSPEFEARRKAALTAKAQTPEGYAYFAQRGAENFRRYRE
                     QHPDAARLSAIANKNGERGKQYLVAYNQSEAGRIKSSEVAHRVHTCEACGEGVPGGGF
                     GIHNHRRWRHGFNHKVVSVERLNERADVYCLNVPEYGNFALDAGVFVHNCGMMSARS"
     gene            complement(1424..2257)
                     /locus_tag="VF240_21275"
     CDS             complement(1424..2257)
                     /locus_tag="VF240_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177805.1"
                     /translation="MSKLAAARLIFAALLALSLTCAAGAQTSGRRGAAAASKGEVLYD
                     YRTDLPRPPVVVVGAAEEDGVLDATFKKYLPDPARCAPDASPASEDDLADLRASGQFA
                     PSVSEAVRGSFTGPARQETLYIIAVGECGDLPRSLGNGSSQLVIRRGGENVLSLNGYY
                     GTVSAVRDVDGDGVHELLLRVGGSGQGVIEESAQLLSFAGGRERVVHDFENVRVDTCG
                     QGERTSDIKAALLRFVAPPPGAGAATRFETDFYVSRCYEEKDRPRLKDFRFLKRGKLY
                     D"
     gene            complement(2250..2831)
                     /locus_tag="VF240_21280"
     CDS             complement(2250..2831)
                     /locus_tag="VF240_21280"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7177806.1"
                     /translation="MAGHVRAGAASESDTPDAPPRVVVITGFMGAGKSTVARELGRRL
                     SCLVIDLDDLITGRAGRTPRELIDEEGETHFRELETDALREAMTGDGSVVVATGGGAW
                     AHARNRSLADARGCLTVWLDAPFELCWRRITNAPGTRPLARDETQARRLFDERRATYS
                     LARLRVEVRDDTTPETLSAAIADALEIDGSTHE"
     gene            complement(2855..4234)
                     /gene="aroA"
                     /locus_tag="VF240_21285"
     CDS             complement(2855..4234)
                     /gene="aroA"
                     /locus_tag="VF240_21285"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7177807.1"
                     /translation="MRIRPARRLRGHIGGLPGDKSISHRAAIIAALARGRTRVRNFST
                     SADCASTLECLRRLGVQVEREGDSADGALIIDGAGGEEAGRWSLRAPEGALDCGNSGT
                     TMRLLAGVLAAQDFTATLTGDASLSERPMRRVVEPLELMGARVQTEEGHAPLRVEGRR
                     PLRAVTYEMPVASAQVKSCILLAGLGAEGDTRVVEQLAPTRDHTERLLEWFGARVRVQ
                     DAPEPNERRELSAHQSSVTVEGPALLRARDVTVPGDISSAAFLLAASALLPESDLFID
                     GVGLNPTRAGIVRALRALGADIRVVNAHDECNELVGGVRVRGAGALRRAAGRDGAGTL
                     RGAAVARLIDELPVLAVVGTQVEGGLEIRDAGELRVKESDRIDATVRNLRAMGAEVEE
                     FDDGLRVGGPTRLRGARLASFGDHRIAMAFTVAALVASGESLIEGAGECVPVSFPEFY
                     ELLESVVER"
     gene            4361..6217
                     /locus_tag="VF240_21290"
     CDS             4361..6217
                     /locus_tag="VF240_21290"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7177808.1"
                     /translation="MKLSPSPTYSRGARSRRLALSVVAFIIAHVAAFVLAPAARALSS
                     SSCVAARGGGAQEAAPQKQREADARAGERAHSIVRGRVVYDDTNRPVRRAQVHIFDPN
                     LRSNRAPRMVWTNARGEFVVRNLPAGSYYVVVKAPGVALGFFDGPAAQREQDATTATI
                     DGTNNTEVRVRIKRGGAISGKITYADGEPAAGATLSLRRKKDAQLLPVYLSGGYGHSV
                     SVDDRGLYRIAGIPPGEYVVGAAEQKMSVSETDEDGGRRGYQTALSVTFYPGALSPQQ
                     ATTVTVEAGQEVEDIDITLVERNTHAVSGAVVERGTQRPVHGAEVSLTSKDDSGVREP
                     SNAPTTQTDEQGRWSLEEVQEGGYTLTVVPQYDYRSRVEAGAAAGPVPRPKFVARRQE
                     LQVAGGDIAGLVVEVSAGARVSGRVAVEGGKPLPPDVWVYMEPAQRGESPLALHQAQA
                     GPDGTFTIEGLSAGDYHLTVRAPREGAFYAKSITAAGGADLLSAPLGVEEGAAVRGVR
                     VVLSPDVATLTGRVLSTQGAPLRGAVLVLLSADPTRRRLHTARLHATTSADGGFRVSG
                     APGEYVVLVFRPEQLTREPGEAAVEARAASAPRVTLAPGESKRADFTAPGDK"
     gene            6457..6870
                     /locus_tag="VF240_21295"
     CDS             6457..6870
                     /locus_tag="VF240_21295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177809.1"
                     /translation="MTEETTELSFPSRVEAIAEAAEAVVDVARRAGFTEEALYGVDMA
                     VRESATNAVLHGNSLDAAKTFEVGVFVQGDRLVVTVRDRGEGFDTSAVPDPTDPQNLL
                     KASGRGILFMRTFMDEVGWERHPEGGTVVRMTKKK"
     gene            6981..7325
                     /locus_tag="VF240_21300"
     CDS             6981..7325
                     /locus_tag="VF240_21300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7177810.1"
                     /translation="MAELNIRERQAGDVTILDMDGKITIGEGSVALRSAVRRLIEENK
                     KKILLNLANVGYVDSSGIGELVSSFTTIQREGGQLKLLKLTQKIRDLLGITKLLTVFD
                     SFDDESEALNSF"
     gene            7460..7939
                     /locus_tag="VF240_21305"
     CDS             7460..7939
                     /locus_tag="VF240_21305"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX7177811.1"
                     /translation="MFRGAFRRLLALDDPPERTALAFSIGIFIAFSPFLGLHTLMATV
                     VAFAFRFNKVAIYTGTFVNNPFLTLVPIIVVSYAVGALLTGRPLALPPEGMELLSDPH
                     LFNRDYWRRLGAHAWDLVLPFSVGAMALSVVCSLAAYPVTLRLLRAHRNRRKGTGDK"
     gene            8105..10006
                     /locus_tag="VF240_21310"
     CDS             8105..10006
                     /locus_tag="VF240_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177812.1"
                     /translation="MRKPYFFVAVALLCVQTAAFAQNNAKPVATAPTPAPAARRPQTT
                     PQQQQQPRAQVPRPASFDLTDYGVRIEPEPRLVVMMAALDAAGWDPTPAGQEPTLFRR
                     TVREDQASLDPALRARLRDFFARYKPPAPATAAEAAAPYVSLTYLLGPAPAFDAPPRS
                     DDLPAGVLDVLDFVPLLREFYRQTAMEERLPRYVRMHHEEGNRLRQRTAEMVKEVLTY
                     LHTRPQLTIIERTQTRAPASKDDKKRDAPRPTTTLREKERRFLIVPDLLAAPGAINFR
                     VIGDDYHTILPAGTDPTSSELRRAYIQYVADPLVLRAGRDISAKRLAVKQLLDEQRAR
                     NPNVTPDVFLSVARSFVIAAEARIEELARVRALQLSAAARLKSVGTEEAARAVVGREV
                     QAERVRIEDETVARLAEGYERGAVLSFHFAEQLRGLETSGFDISNFFGDMIGAIDAVR
                     ETRRLAESAETVKRHRETRERARQESAERMAREAAAAPTVSEGRQALLKGLGEADELL
                     RLGDYARAEEQLRALQKQYPTEPHVYFALARTASRSAEGAFDPALQTQRLGLALQLYQ
                     DAIRAASPDTDAALISRAHVASGRILAHLERAADALKEFDAAIRIGAVEGGALREAQA
                     ERQKLTPQP"
     gene            complement(10110..11399)
                     /locus_tag="VF240_21315"
     CDS             complement(10110..11399)
                     /locus_tag="VF240_21315"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7177813.1"
                     /translation="MTKKAVLTLSGLLLLTQGHALRAQDTPAAPPSPSTPAVAAVAPA
                     GPPPQAAAVVAPAPPAQGVSAVSVPQAAPPTLAPAQAPPPSAVLAPAPPTHAVPAMAY
                     APAPPQGPGEVTIFHFGDNFLGVSVEDVTRENMGRYNQSGEPRGVGVRSVVKGGPAEK
                     AGLRENDVILRFDGETVTTTRKLNRLLGESAPEHTARLTIRRGGGEQEISVTLGKREF
                     GREFGGTMIDGELWRRQAEEMSRNSEQWKRQGEEARRKMEEWQRKNPGGVFTFFGGGR
                     RVGISTNALGKQLADYFGVEHGALVTSVEPDSPAARAGLKAGDIVTDVDGERVEDAGD
                     LSRAINRKEEGEVTLTVVRERNRRTVRVTPEKMKSPGGELFPGGLRIEAPSIAISMPQ
                     VRVAIPPINLSLPSINIQPRPRIAPRVIRPRSGPTIL"
     gene            11525..12139
                     /locus_tag="VF240_21320"
     CDS             11525..12139
                     /locus_tag="VF240_21320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7177814.1"
                     /translation="MLTKDTAEDGAADVRAAATTPGAMKILDIGCGRHKTPGAVGIDS
                     NPRSDADVLHDLDSVPYPFPDDEFDLIIGNQVIEHVADILAVMAELYRIARPGAVIRL
                     DTPHYSDIASYTDPTHRHHLTTESFSYFTGRRTDFDFYSEVRLRPLVVRVTMLKLWRA
                     LGFEFLVNLGNRYPSMRFLRKFWESYLCFVVRGKTIYFEFEVIK"
     gene            12207..12728
                     /locus_tag="VF240_21325"
     CDS             12207..12728
                     /locus_tag="VF240_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177815.1"
                     /translation="MATGTKRGKQAEGEQPKRAARPSRKDQILALYLSGMGEIEDIAM
                     ITGARPSYVGSVLQAAGLNRGYFDLYTSTSNPMNVYSKFFAGKLGFKDEAVARESVNV
                     IDRAHRQFDLAGDRAGQHHALQMALTMFDRARFTGKGREAAVFRDWLAARLSEADLEA
                     TDDALVDGDEEAG"
     gene            13068..14495
                     /locus_tag="VF240_21330"
     CDS             13068..14495
                     /locus_tag="VF240_21330"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7177816.1"
                     /translation="MTDKTTEQATAALPREMNLRDLVLFNLAALIGLTWVSTAARAGP
                     SAITLWLVAALLFFIPQGLAVVELSSHFPDEGGIYAWTKRQFGVGHGFLCGWCYWINN
                     VLFYPSVLFSIASLTAYAIDPGGDLSDRWTFILPFTLIMLWAAATLNAVGVGRGKWLQ
                     NCGGASTYVAGAVLIAFGLYGVLTAEPANAFTLESLRPSFADVSSLNFWATIAFAFAG
                     LELSSTMGSEIKEPRRNLPLSIYIAAPLAALLYILGTGAVLWYLPAGEIEIIPAPFQA
                     IAVGAARLSASLWWLAPAVAALAAFGRLGGLGAWLTGSARVAFTVGLDRYFPAAFGRV
                     HPRWRTPHVAIFVQAAIATLFVFFAVLGRGTTVERAFLVLIDMSLLIYFIPYVYLFLC
                     FILHLRRTPPERVQIPGGRMAGMLLGVCGLLTTLFAMAVALIPPPDTPSPGMFILKVG
                     GGSLLIVLVSGLIYWRARHRRVEAN"
     gene            14532..16166
                     /locus_tag="VF240_21335"
     CDS             14532..16166
                     /locus_tag="VF240_21335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7177817.1"
                     /translation="MPVDETDELTLSDERTGAIAFSRGARVALVLVVCLYVAARVWRL
                     TDSCLWFDEIFGVHAARHSWGRLLGFAAADLIHPPLFYALLKLWINVGGESLPWLRLL
                     SVLLSIASVAPLVLLCRELRLGAWETNLALALAAVNGYLIKYAQEVRMYSLLLLLTLC
                     SLWLLARFFNASDERRSRQTLVALAAGNLLLVYTHYYGWLVVASEAALLLLLRRGRKR
                     ARLAPFLLTVALAAVAYLPWVYALSQQRGEQGRALAQNIGWVERPAPTDVLQFLALLH
                     EPFYFRRSSADPVYTKLTAPAGLLLFGVPILLLLWRTAKRARAGDGARRGALCWLLVF
                     SFLPLLLAFALSWALPHSIWGTRHLIVVAAPYLVMAAAAVVRLRPEWARAAVLVVLGC
                     WVFWGALGALVRREAAPVWCAWEGHAARMRRAETAAPAGVVSVYAFEDLVAYHLWFAL
                     EGAGDGRFRVSVVKGVPGLAEDPAYFLPRAFDGVGVGDASALEGARIWVAFRAAAWDE
                     TRAPLKTVLERGYRVEEVYETSAQGQRAFMVSLRKD"
     gene            16306..16938
                     /locus_tag="VF240_21340"
     CDS             16306..16938
                     /locus_tag="VF240_21340"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX7177818.1"
                     /translation="MDSEKRKHLILLGVGLAALAASAVRPFDWLTWVLEVAPAILGLA
                     IILFVYRRFRLTTFAYVCVLIHALILFLGAHYSYAKAPPGFWVQDLFDLSRNHYDRLG
                     HFAQGFFPAIYTREIFLRLRVVKRGWLFFIVTAVCLGISAFYEFTEWWAALAGGDSAE
                     AFLGTQGDQWDTQWDMFLCAVGAVLSQLLLGRVHDRAVAHMSAVNAPEER"
     gene            17098..17538
                     /locus_tag="VF240_21345"
     CDS             17098..17538
                     /locus_tag="VF240_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177819.1"
                     /translation="MESADRVRANTSEEVNCRIDRELDTRVREYAHRSPDDITRRISE
                     LDREWDMERLLETNASTVAFTGLVLGVTHSKKWLIVPGIVLPFLFQHAVQGWCPPVPI
                     LRRLGVRTREERDREKYALKVLRGDFDNADSLPRAEAALQSVKA"
     gene            17567..18691
                     /locus_tag="VF240_21350"
     CDS             17567..18691
                     /locus_tag="VF240_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177820.1"
                     /translation="MTAVHVRALSTDEELRRYVDFGQEVYRQNPNWVPPDAHHLTELL
                     SGRSAFGAQSQVQAFWVEEGERVLAVVTAIRDEVYTRHWNEQTGHLLFFEALPRCDEA
                     VESLFRAAGDWLRERGCRAARLSFLAGWQMPLTIDAYDDVPTIFHTYNPAYYHSYIKN
                     AGFRTEYGVVQYQIQFTPELAGRYREMIERVTNSGVSLRSWDFDRPEEENQTFTDIAN
                     ETFRAHWGFNPMPASALRGLTVELKDFLVADFIVFAEAEGQTIGAVYSLPDLNQAFHR
                     MRGKAIEENLPELQQRLQDIDHGVLLIIGVKESHRGRGVNLALAAKSYLAMIERGYKT
                     GSYTVVLDDNWPSRRTAEKLGARVTRNFNIYRKELTRPSA"
     gene            18834..19043
                     /locus_tag="VF240_21355"
     CDS             18834..19043
                     /locus_tag="VF240_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177821.1"
                     /translation="MADQKDKAQEKKRQEESAGSEQNDEQASGHGSGGGNMGAGKSGK
                     EGMGSEKPSDDERMGSHKPGGSGSQ"
     gene            complement(19128..20084)
                     /locus_tag="VF240_21360"
     CDS             complement(19128..20084)
                     /locus_tag="VF240_21360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7177822.1"
                     /translation="MRIVPIVVPTPFYVGPVNCYLVVAEPLTLIDTGPKTKEALEALK
                     DGLRRERVRPSDIRRIVLTHAHEDHCGLAKSLRDEAKDAEVLVHGWETGHRIGRLEYE
                     EHRALLVRAGVPEDEIEGMRRVYEQVRQLADALEDDEHSELYDEEDLEFANGSLRVVH
                     TPGHTPGSCSFFREADRTIIAGDCVLKRITPNPILSPDPIDPGRRFRSLEEYLVSLSR
                     LRALHPTLVLGGHGEPVHDYEELFNRYLRAVRERQSDVMKLIPKEGATGWEVARGLFP
                     DTDDVHRFLSVSESVAHLDLAHTDRKVEVELTSDGREVYRRA"
     gene            complement(20193..21980)
                     /locus_tag="VF240_21365"
     CDS             complement(20193..21980)
                     /locus_tag="VF240_21365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7177823.1"
                     /translation="MSAVAEEKKVFAGGSFLIEERAPAEVFTPEDFTEEHRMIAETTR
                     QFMDNEVRPRTAELEKHDWKLARELVRKGAELGLVGATVPEAYGGLGMDQTTGAVIGE
                     LLGRSASFATTMGAQSGIGLLPIVYFGSEEAKQKYLPKIVSGELVTAYALTEAGSGSD
                     ALAAKATARLSDDGTHYILNGEKMWITNGGFADIFVVFAKVDGEKFSGFIVERQEGLT
                     NGAEEHKMGIKGSSTTALVLSDVKTPVSNLLGEVGMGGKIALNTLNIGRFKLGAMCGG
                     GMKLMTQEAVRYANERHQFGKPISSFGAIKAKLGEMAVRTWVGESMNYRTLGLLDAGL
                     AALGADASMLDKGRTIEEYATECSIIKVALSEYCDFVADEMVQIFGGYGYSADYPAER
                     AYRDSRINRIFEGTNEINRMLIPGMLMKKAMKGQLALLPAAQALMDEVLSPSMSGMDE
                     DEGPLSNELRLARNAKKVALMVLGTAAQKYMMALADQQEVLLGVADIIMDVYAMESAI
                     LRTRKMTDAKGEDASARYLDITRVFCEGAAARIETRAKETLAAMAEGDELRTLLAALR
                     RFTKNTPMNTVAARQRIADAMITANKYVY"
     gene            complement(22121..23299)
                     /locus_tag="VF240_21370"
     CDS             complement(22121..23299)
                     /locus_tag="VF240_21370"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7177824.1"
                     /translation="MRDAVIVSAVRTAVGKAPKGTLRNTRPDEMGAAVIKEALARAGG
                     VEAAEVEDVVMGCAMPEAEQGMNVARAAAVRAGLPVETSAMTINRFCSSGLQSIAIAA
                     DRIRTGAAEVCVAGGLETMSLIPMGGHIIRPNPHLVEHYPDFYLNMGLATENVARKYE
                     VSREEQDAFATLSHRRAAAAQDAGKFDDEIVPLSVTFEELNDKGKKTTREVSFDKDEG
                     VRRDTSEEGLAKLRPAFHTGGTITAGNASQMSDGAAAVVIVSDERARQLSLKPLARFV
                     AYATAGCPPEEMGIGPVHAIPKVLKLAGLRLEDIDVIELNEAFAAQSLAVIRTLVLDP
                     ERVNVNGGAIALGHPLGCTGAKLTATALRELERREGRYAMITMCVGGGMGAAGIFERL
                     "
     gene            complement(23384..>25047)
                     /locus_tag="VF240_21375"
     CDS             complement(23384..>25047)
                     /locus_tag="VF240_21375"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7177825.1"
                     /translation="RLFDLVGLDVFMHVAKNLYEAVPEDEDRDAYLAPDFARGMVERG
                     LLGNKTKGGFYRRQKGEGGKQEIWTLDVATLEYRPAEKVKLPALDAAKNIEDTRERLR
                     TLTWGKERAGAFLWKTISKTFVYAANRIPEIADTVLEVDRAMKWGFNWELGVFEAWDA
                     IGVEKSVARLEEEGRKVPENVRRMLEAGAKSFYKSEGGQQSYFDFATGAYVPVAEQPG
                     VVVLKSVKDRTGVVKKNAGASLIDIGDGVACLEFHSKMNSIGGDTLQMVKTALAEVER
                     NFVGLVIGNQGQNYSVGANLMLVLLGAQEEEWEEIDLGVRAFQQATMSLRYSAKPVVV
                     APFGMTFGGGCEMTLHADRVRAAAETYIGLVEVGVGLIPAGGGTKEMAMRAGDSIPPG
                     AEEADPFPFFKRAFETMAMAKVATSAEEARKLGFLRDEDSYSMNSERLIADAKQEVLA
                     LHQSGYTRPQPRTDVFAPGLSALSTLKLGIHQMKRGGFISDYDALIGEKLARVVTGGD
                     LNHPTRVSEQYLLDLEREAFLSLCGQRPTQERIAHMLKTGKPLRN"
BASE COUNT         4028 a         8450 c         8077 g         4492 t
ORIGIN      
        1 gagcgcgcgc tcatcatccc gcagttgtgg acgaacacgc ccgcgtcgag cgcgaagttg
       61 ccgtattcgg ggacgttcag acagtaaacg tccgcccttt cgtttagcct ttcgacggaa
      121 acgactttgt gattgaaccc gtgtcgccag cgccggtgat tgtgaatacc gaacccgccg
      181 ccgggcactc cttcgccgca cgcctcgcaa gtgtgtacgc ggtgcgccac ctccgaagac
      241 ttaatcctcc ccgcctcact ctgattgtag gcaactaaat actgtttccc gcgctcgccg
      301 ttcttgttgg cgatggcgct aaggcgagcc gcgtccgggt gctgttccct gtaccttcta
      361 aaattctccg cgcccctttg cgcaaagtaa gcgtagcctt ccggcgtttg tgctttagcc
      421 gtcagggccg cctttcgtct ggcctcaaac tccggcgaac ggaaatgcgg gttacgctcg
      481 gcaatcgaat ggtgatagct caagtggtca aggtgcccca tgaactgaag attctccggg
      541 cgattgtcag acgggagaaa attcctgtga tgaatgattg tttgctgccc ggcgaaagca
      601 gggatatctc cgagcaaccc ctgtcgcgcc atgacccaat gcactctctg attcgtgccg
      661 gtggcaggat gcctgaccat cgtatagcca tccttgtcgg cacgcatgtc gaacggcatc
      721 agagaagcgc cgggctgcaa ctcctgtgct tgccggtaat cgccatcgcg cagcatgaac
      781 tcatgatccg gcgtgcagaa aatttctctg ccgttgtcga gcgtcacctt cacgagcggg
      841 gcgtctgtgc gcgtccttct tgcggtcgcc ttagcgacca cgattttccg agtctccgag
      901 agcacataga cgtgaatctc ctcgccggac tcggccaacg cgccaatcgg ataagagtgc
      961 ccgtcggcgg ttggcacgag cgtgtcggcc ctgaagcaac caatgtcgaa accgaccggc
     1021 cccatcgcca ccgccccgct ttcggcgtcg gtgaggatca cgcagccgac gggcacgccg
     1081 tagccgtagt gtacgtcggg ggtgagcacg accagacgca cgccggggaa gcgcgtggcg
     1141 ttgatgatct gtcggtagag gatgtcctcg gcgtccgcga ggagttgcgg cgtgagaaac
     1201 attcgcacgg gcacgtcacc cgtgtcgtct gtttgcaatt cgtagtagcc gctgccatgc
     1261 cagcgcgcga ggtgcttcca gttgtgcgcg ctcttcagtt ctttggcgac cttcagtgct
     1321 ttcgcgtccg tctctttgtt cgtcatagct tgctcacctt cgttcgagaa aactaacttt
     1381 gccgcggcgc actcccgcgt cttcgcgcgg caaaagcgga gtatcaatcg taaagcttcc
     1441 cgcgcttgag gaagcgaaaa tctttgaggc gcgggcggtc tttctcttcg tagcagcggc
     1501 tgacgtagaa gtccgtctcg aagcgcgtgg cggcgccggc gccgggcggc ggtgcgacga
     1561 agcggaggag ggcggccttg atgtcgctcg tgcgctcgcc ctgtccgcag gtatcgacgc
     1621 gaacgttctc aaagtcgtgg acgacgcgct cgcgcccgcc cgcgaagctg agaagctggg
     1681 ccgactcttc gatcacgccc tgaccggaac cgcccacgcg cagcagaagt tcgtgaacgc
     1741 cgtcgccgtc cacgtcgcgc acggcggaga ctgtgccgta gtagccgttg agagagagga
     1801 cgttctcgcc gccgcgccgg atgacgagtt gcgaggagcc gttgccgagg gagcgcggca
     1861 ggtcgccgca ctcgccgacg gcgatgatgt aaagcgtctc ctgccgggcc gggccggtga
     1921 acgagcctct gaccgcctcg gagacgcttg gggcgaactg cccgctcgcg cgaaggtcgg
     1981 cgaggtcgtc ttcgctcgcc gggcttgcgt caggcgcgca tcgggcgggg tccgggagat
     2041 actttttgaa cgttgcgtcg aggacgccgt cttcttccgc cgcgccgacg accacgacgg
     2101 gcggccgcgg caggtcggtg cgatagtcgt agagcacttc gccctttgac gccgctgccg
     2161 ccccacgccg gccggacgtt tgcgccccgg ccgcacacgt caacgagagt gcgagaaggg
     2221 ccgcgaatat caaccgcgct gctgcaagtt tactcatgtg tgctcccatc gatttcgagc
     2281 gcgtcggcaa tcgcggccga cagagtctcg ggtgtcgtgt cgtcgcgcac ctcgacgcgc
     2341 aggcgcgcga ggctgtaagt tgcgcgccgc tcgtcgaaga ggcggcgcgc ctgcgtctcg
     2401 tcgcgcgcga gggggcgcgt gcccggcgcg tttgtgatgc ggcgccagca tagctcgaag
     2461 ggcgcgtcga gccagacggt gagacagcca cgcgcgtcgg cgagcgagcg gttgcgcgcg
     2521 tgcgcccacg cgccgccgcc cgtggcgacg accacggagc catcgcctgt catcgcctca
     2581 cgcagcgcgt cggtctcaag ctcgcggaag tgcgtctcgc cttcttcgtc gatcagctcg
     2641 cgcggggtgc ggcccgcgcg cccggtgatg aggtcgtcga ggtcgatgac aagacaggag
     2701 aggcggcgtc cgagttcgcg tgcgaccgtg ctcttgccgg cgcccatgaa gccggtgatg
     2761 acgacgacgc gcggcggcgc gtccggcgtg tcgctctcgg aggccgcgcc cgctcgaaca
     2821 tgtcccgcca ttcttccgcg gccgacgttt ctcgtcatct ctccacgacc gattcgagca
     2881 gttcgtaaaa ctcggggaac gagaccggga cgcactcgcc cgcgccctcg atcaaggatt
     2941 cgccggaggc cacgagcgcc gccaccgtga aggccatcgc gatgcggtga tcgccgaacg
     3001 acgcgaggcg cgcgccgcgc agccgcgtgg ggccgccgac gcgcagccca tcgtcgaact
     3061 cctcgacctc cgcgcccatc gcgcgcagat tacgcacggt cgcgtcgatg cggtcggact
     3121 ccttcacccg cagctctccg gcgtcgcgta tctcaaggcc gccctcgacc tgcgtgccga
     3181 cgacggcgag caccggcaac tcgtcgatca aacgtgcgac cgccgcgccc cgcaacgtgc
     3241 ccgcgccgtc ccgccccgcc gcgcgtctga gcgcccccgc gccgcgcacg cgcacgccgc
     3301 cgactaattc gttgcactcg tcgtgcgcgt tcaccacgcg gatgtccgcg ccgagcgcgc
     3361 gcagcgcccg cactatgccg gcgcgcgtgg ggttgagtcc gacgccgtct ataaacaggt
     3421 ctgactcggg cagcagcgcg gaggccgcga gaaggaaggc ggccgaggag atgtcgcccg
     3481 gcaccgtcac atcgcgcgcg cgcaagagcg ccgggccttc gacggtcacg gaagattggt
     3541 gcgcggaaag ctcgcggcgc tcgttcggct ccggcgcgtc ctgcacacgg acgcgtgcgc
     3601 cgaaccattc gagcaggcgt tccgtgtggt cgcgtgtcgg cgcgagctgt tcgacaacgc
     3661 gcgtgtcgcc ttcagcgccg agaccggcga gcaggataca cgacttgacc tgcgcgctgg
     3721 cgacaggcat ctcgtatgtg acggcgcgca gcggacggcg gccctcgacg cgcagcggcg
     3781 cgtggccctc ctccgtctgc acgcgcgcgc ccatcagctc cagcggctcg acgacgcggc
     3841 gcatggggcg ctcgctcagg ctcgcgtcgc cggtgagcgt cgccgtgaag tcctgtgcgg
     3901 ccagcacgcc ggcgagcaga cgcatcgtcg tccccgagtt gccgcagtcg agcgccccct
     3961 cgggcgcgcg caaagaccag cggccggctt cctcgccgcc ggcgccgtcg atgatgagcg
     4021 cgccgtcggc gctgtcgcct tcgcgttcca cttggacgcc gagacggcga aggcattcga
     4081 gcgtcgaggc gcagtcggcg ctcgtcgaga agttgcggac gcgcgtgcgg ccgcgcgcga
     4141 gcgccgcgat gatggcggcg cggtgcgaga tcgacttgtc gccgggcaag cccccaatgt
     4201 gtccgcgcag gcgccgcgcg ggtcgtattc tcattccggg attttaaacg atggcggaat
     4261 aaaggcgaac gccgcggctg cgaaaaggtt tataatcgcg tccgcctcgg tttcttttcg
     4321 gtcgcctcgg caaactctca ctcggaaggc gccgccgctt atgaaactct caccttcacc
     4381 gacgtattcg cgcggcgcgc gcagccgcag gctggcgctc tccgtcgtcg cgttcatcat
     4441 cgcgcacgtc gctgcgttcg tcctcgcgcc ggccgcgcgc gctttgtcgt cgtcgtcgtg
     4501 cgtcgcggcg cgcggcggcg gcgcacagga ggccgcgccg cagaagcaaa gggaagcgga
     4561 cgcgcgcgcc ggcgagcggg cgcattcgat tgtccgaggc cgcgtcgtct acgacgatac
     4621 gaaccggccc gtgcgccgcg cgcaggtgca catcttcgac ccgaacctac gttccaaccg
     4681 tgcgccccgg atggtctgga cgaacgcgcg cggcgagttc gtcgtgcgca acctgcccgc
     4741 gggcagctac tacgtggtgg tcaaggcgcc cggcgtcgcc ctggggtttt tcgacgggcc
     4801 agccgctcag agggagcagg acgcgacgac tgccaccatt gacgggacga acaacaccga
     4861 ggtgcgcgtg cgtatcaagc gcggcggcgc catctcgggc aagatcactt acgcggacgg
     4921 agagccggcg gctggcgcga ccctctctct gcgccgcaag aaggacgctc agttgctccc
     4981 ggtctacctc agcggcggct acgggcacag cgtctccgtt gacgaccgcg gactctaccg
     5041 catcgccggc atcccgcccg gcgagtacgt cgtcggcgcg gccgagcaga aaatgagcgt
     5101 cagcgagacg gacgaagatg gtggccgtcg gggttaccag actgcgctct cggtcacctt
     5161 ctaccccggc gctctcagcc cgcagcaggc cacgaccgtg acggtcgagg cgggccaaga
     5221 ggtcgaagac atcgacatta cgctcgtcga gcgcaacacg cacgccgtct cgggcgctgt
     5281 ggtcgagcgc ggcacgcagc ggcccgtaca cggcgccgag gtgtccctca ccagcaagga
     5341 cgattcggga gtgcgggagc cttcaaacgc gcccacgacg caaaccgacg agcagggccg
     5401 ctggtcgttg gaggaagtgc aggagggcgg ctacaccctc actgtcgtcc cgcagtacga
     5461 ctaccgctcg cgcgtggaag ccggggcggc ggctggccct gtgccgcggc cgaagttcgt
     5521 ggcccggcgg caggagttgc aggtggcggg gggcgacatc gccggcctcg tcgtcgaagt
     5581 ctcggcgggc gcgcgtgtga gcggccgggt cgcggtcgag ggcggcaagc cgctcccgcc
     5641 cgacgtgtgg gtctacatgg agccggcgca acgcggcgag tcgcccctgg ctctccatca
     5701 ggcgcaggct ggccccgacg ggacgttcac catcgaggga ctctccgcgg gcgactacca
     5761 cctcaccgtg cgcgccccgc gcgagggcgc cttctacgcg aagtcgatta cggccgccgg
     5821 cggcgcagac cttctgagcg caccgctggg cgtcgaggag ggtgccgcgg tcaggggcgt
     5881 gcgcgtcgtc ctgtctcccg acgtagcgac cctcacgggc cgcgtcctct ccacccaggg
     5941 cgcgcccctg cgcggggccg tcctcgtgct cctgagcgcc gacccgacgc gccggcgtct
     6001 gcacaccgcg cgcctgcacg ccacgaccag cgccgatggg ggcttccgcg tgagcggcgc
     6061 gcccggcgag tacgtggttc tggtcttccg ccccgaacag ttgactcgcg agccgggcga
     6121 ggcggccgtc gaggcgcgcg ccgcctcggc cccgcgcgtc acgctcgccc ccggcgagtc
     6181 gaagcgcgcg gactttaccg cgcccggcga caaatgaaga aggatgaggg cggagggcgg
     6241 agggatgaaa gcctgaagcc gggcttgaat cattcaaccc gcgccgccgc cccttcatcc
     6301 ctcatccctc cgccctcatc ctttgtcttt cttgacacgc ccgccgctcg cgtgttacac
     6361 cgtccaagct tccggtaagt atttttcaag cgttttgacg cgcgacgcgc ccccggcgag
     6421 ggtgggaggg gcggtcgggg tcgcgtcttc caaaccgtga ccgaagagac gaccgaactc
     6481 agttttccga gccgcgtcga ggccatcgcc gaggcggccg aggccgtcgt ggacgtggcc
     6541 cgccgggccg ggttcacgga ggaggcgctc tacggcgtgg acatggccgt gcgcgaatcc
     6601 gccacgaacg ccgtcctcca cggcaactcg ctcgacgctg cgaagacctt cgaggtcggc
     6661 gtctttgtgc agggcgaccg gctcgtcgtc accgtgcgcg accgcggcga gggcttcgac
     6721 acctccgccg tgcccgaccc gaccgacccg cagaatctcc tgaaggcgtc gggccgcggc
     6781 atcctcttca tgcgtacctt catggacgag gtcggctggg agcgtcaccc cgagggcggc
     6841 accgtcgtcc gcatgacgaa gaagaagtag taagcagttt tcagttttca gtcctcagtt
     6901 taaaagcctt tcggccggcg cctgaaactg acgactgaaa actgaaaact gaaaactgtt
     6961 tttcaaggag actatccgca atggctgaac tgaacatccg cgagcgccag gccggggacg
     7021 tgaccatcct cgacatggac ggcaagatca ccatcggaga gggcagcgtc gcgctccgca
     7081 gcgccgtgcg ccgcctgatc gaagagaaca agaagaagat tctgctcaac ctggccaacg
     7141 tcggctacgt cgattcctcc ggcatcggcg agcttgtctc cagcttcacc accatccagc
     7201 gcgagggcgg ccagttgaag ctcctcaagc tgacgcagaa gattcgcgac ctgctcggca
     7261 tcaccaagct cctgaccgtc ttcgactcct tcgacgacga gtccgaggcg ctcaacagct
     7321 tctgagagaa tcgtgagtcg tgaatcgtaa atcgtcaatc gtgattggcg ctctccccgc
     7381 ctgagagaca cgtcggaagt tggccgccgc tcaacttcta ttcacgattg acgattcacg
     7441 attgacgatt cacgtctcta tgttccgtgg tgccttccgc cgcctgctcg cgctcgacga
     7501 cccgccggaa cggacggcgc tcgccttctc catcggcatc ttcatcgcct tctcgccctt
     7561 cctcggcctc cacaccctga tggcgaccgt cgtcgccttt gctttccgct tcaacaaagt
     7621 cgccatctac accggcacgt tcgtcaacaa cccgttcctg acgctcgtgc ccatcatcgt
     7681 cgtctcctac gccgtcggcg cgctcctcac gggccgtccc ctcgcgctgc cgcccgaagg
     7741 gatggaacta ctctccgacc cgcacctctt caaccgcgac tactggcgcc gtctcggcgc
     7801 gcacgcgtgg gatttggtgc tgcccttctc ggtcggcgcc atggccctct ccgtcgtttg
     7861 ttcgctggcc gcctaccctg ttaccctacg cctcctgcgc gcccaccgaa acaggagaaa
     7921 ggggacaggt gataagtgat gagtgataag tgataagaaa agccgtcttc tctttcttat
     7981 cacttatcac tcatcactca tcaccgtctc actggcatcc ggccgaaggt gggaggcatc
     8041 tttacttttg ccgtgagccg aaaatcgcat ctttcaacct acacgtccgt gccttaatgg
     8101 atttatgaga aagccgtact tcttcgtggc cgtcgccctg ctatgtgttc agacggccgc
     8161 gttcgcacag aacaacgcga agcccgtcgc cactgctccg acgcccgcgc ccgctgcccg
     8221 gcgtccgcag accacgccgc agcagcagca gcagccgcga gcgcaagtcc cgcggcccgc
     8281 ctccttcgac ctcaccgact acggcgtccg catcgagccg gagccgcgac tcgtcgtgat
     8341 gatggccgcg ctcgacgccg ccggctggga cccgacgccc gcgggtcagg agccgacgct
     8401 tttccgacgc accgtgcgcg aggatcaggc gtcgctcgac ccggccctgc gcgctcgtct
     8461 gcgcgacttc ttcgcgcgtt acaaaccgcc ggcgccggcc accgccgccg aagccgctgc
     8521 cccctacgtc tcgctcacct acctgctcgg ccccgcgccc gccttcgacg ccccgccgcg
     8581 ctccgacgac ctgcccgcgg gcgtcctcga cgtgctcgac ttcgtccccc tgttgcgtga
     8641 gttctaccgg cagacggcga tggaggagcg actgccgcgc tacgtgcgga tgcaccacga
     8701 ggagggcaac cgcctgcgcc agcgcacggc cgagatggtc aaagaggtgc tcacatatct
     8761 ccacacgcgc ccgcagctca ccatcatcga gaggacgcag acgagagcgc ccgcgtcgaa
     8821 ggacgacaag aagagggacg cgccgcgccc cacgacgacc ctacgcgaga aggagcggcg
     8881 cttcctcatc gtccccgatc tcctcgccgc gcccggcgcc atcaacttcc gcgtcatcgg
     8941 cgacgactat cacaccatcc tccccgccgg caccgacccg acctcctcgg agctgcgccg
     9001 cgcctacatc cagtacgtcg ccgacccgct cgtgctgcgc gcgggccgcg acatctcggc
     9061 caagcgcctc gccgtcaagc aactcttaga cgagcagcgc gcgcgtaacc ccaatgtcac
     9121 gcccgacgtt ttcctgtcgg tcgcgcgctc cttcgtcatc gccgccgagg cgcgtatcga
     9181 ggagttggcg cgcgtgcgcg cgctgcaact gtcggcggcc gcgaggttga agtcagtagg
     9241 gacggaggag gcagcgcgcg ctgtagtcgg tcgagaggtt caggcggagc gcgtgaggat
     9301 tgaagatgag acggtcgcgc ggctcgcgga aggctacgaa cgcggggccg tgctctcgtt
     9361 ccacttcgcc gagcagttgc gcgggctcga aacttccggc ttcgacatct cgaacttctt
     9421 cggcgacatg atcggcgcaa tcgacgcggt gcgcgagacg cgtcgtctgg cggagagcgc
     9481 cgagacggtc aagcgtcacc gcgagacgcg cgagcgagcg cggcaggaat cggccgagcg
     9541 gatggcacgc gaagcggcgg ctgctccgac ggtgtcggag gggcggcagg cgctcctcaa
     9601 gggactcggt gaggcggacg agcttctgcg cctcggcgac tacgcgcggg ccgaagagca
     9661 actgcgcgcg ctccagaagc agtacccgac ggagccgcac gtctacttcg cgctggcccg
     9721 cacggccagc cgctcggccg aaggcgcttt cgacccggcc ctccaaacgc aacgtctggg
     9781 cctcgcccta cagctctatc aggacgccat ccgcgccgcc tcgcccgaca cggacgccgc
     9841 cctcatctcg cgcgcccacg tcgccagcgg gcgcatcctc gcgcacctcg agcgcgcggc
     9901 cgacgccctc aaggagttcg acgccgccat ccgcatcggc gccgtcgagg gcggcgcgct
     9961 ccgcgaagcg caggccgagc ggcagaagct caccccccag ccctaagcca gatcgggtaa
    10021 agggtaaagg ggaaggggta agggaaaaga actgtctttc ccttacccct tcctctttac
    10081 cccttgcccc cttgcctgtc ccctgctcgt taaagaatcg tcggcccgct acggggccgg
    10141 atgacgcgcg gcgcgatgcg tggccgcggt tgaatgttga tggacggtag actgaggttg
    10201 atgggcggga tggcaacgcg cacctgcggc atgctgatgg cgatggacgg cgcttcgatg
    10261 cggaggccgc cggggaacaa ctcgccgccc ggcgacttca tcttctcggg cgtcacgcgg
    10321 acggtgcggc ggttgcgctc ccgcaccacg gtgagcgtca cctcgccctc ctccttgcgg
    10381 ttgatggcgc gcgagaggtc gcccgcgtct tccacccgct cgccgtcaac gtcggtgacg
    10441 atgtcgccgg ccttcagccc cgcgcgcgcg gccgggctgt ccggctcgac gctcgtgacg
    10501 agcgcgccgt gctcgacgcc gaagtagtcg gccagttgtt tgccgagggc gtttgtcgag
    10561 atgccgacgc gccggccgcc gccgaagaag gtgaaaacgc cgccggggtt tttgcgctgc
    10621 cactcttcca tcttgcggcg cgcctcctcg ccctgccgct tccactgctc cgagttcctg
    10681 ctcatctcct cggcctgccg gcgccacagc tcgccgtcga tcatcgtgcc gccgaactcg
    10741 cgaccgaact cgcgcttgcc gagcgtcacc gagatttcct gctcgccgcc gccgcgccta
    10801 atcgtcagac gcgccgtgtg ctcgggagcc gactcgccga gcagacggtt cagcttgcgc
    10861 gtggtcgtga cggtctcgcc gtcgaaacgc aggattacat cattctcacg cagccccgcc
    10921 ttctccgccg ggccgccctt caccaccgag cggacgccga ccccgcgcgg ctcgcccgac
    10981 tggttgtaac ggcccatgtt ctcgcgcgtc acgtcttcga cgctgacgcc gaggaagttg
    11041 tcgccgaagt gaaagatggt gacctcgccc ggcccctgcg gcggcgcggg cgcgtaggcc
    11101 atcgcgggaa ccgcgtgcgt cggcggcgcg ggcgcgagca cggcggacgg cggcggcgcc
    11161 tgcgccgggg cgagcgtcgg cggcgcggcc tgcggcacgg acacggcgga gaccccctgc
    11221 gccgggggcg ccggggccac gacggcggcg gcctgcggcg gcggccccgc tggcgcgacg
    11281 gcggcgacgg ccggagtgga tggcgagggc ggcgcggcgg gcgtgtcctg cgcccggagt
    11341 gcgtggccct gcgtgagtag aaggagacct gacagcgtaa gcacggcttt cttcgtcatg
    11401 ggacgactat cctccgttca gaattttcca cccgcttgca cgccccgcac gcccgccggg
    11461 ttccgaaagt tttacgaagg ccccttgtcg gccccgccga gcgggcgcta aacttcacgc
    11521 ccggatgttg acgaaagaca cagcggaaga cggggccgcg gacgttcgcg cggcggcgac
    11581 gacgccgggg gccatgaaaa ttcttgatat cggttgcggc cgtcacaaga cgcccggcgc
    11641 cgtcggcatc gacagcaacc cgcgttcgga cgccgacgtg ctgcatgacc tcgactcggt
    11701 gccctatccc ttccccgacg acgagttcga cctcatcatc ggcaatcagg tcatcgagca
    11761 cgtcgcggac atcctcgccg tgatggccga actctatcgc atcgcccggc ccggcgccgt
    11821 catccgtctc gacacgccgc actactccga catcgcttcc tacaccgacc cgacgcaccg
    11881 gcatcatttg acgaccgagt cgttctccta tttcacgggc cggcgcacgg acttcgactt
    11941 ctacagcgag gtgcgtctgc gcccgctcgt ggtgcgcgtc acgatgctca agctgtggcg
    12001 cgcgctcggt ttcgagtttc tggtcaacct cggcaaccgc tacccctcca tgcgcttcct
    12061 gcggaagttc tgggagagct atctgtgctt cgtcgttcgc gggaagacga tctatttcga
    12121 gtttgaagtg ataaagtagg gacgcacact ggcgagcgtc acggccccca caaagagaag
    12181 atcacaggga aggagacttc aacgacatgg ccaccggaac gaaacgcggc aagcaggccg
    12241 aaggcgagca gccgaagcgc gcggcgcggc cgagccggaa ggatcagata ctcgcgcttt
    12301 atctttcggg catgggcgag atcgaagaca tcgccatgat cacgggcgcg cgtccctcct
    12361 acgtcgggag cgtgcttcag gcggcgggcc tcaaccgcgg ctacttcgac ctctacacct
    12421 cgacctcgaa cccgatgaac gtctactcga agttcttcgc cggcaagctc ggcttcaagg
    12481 acgaggcggt ggcgcgcgag agtgtcaacg tcatcgaccg cgcgcaccgg cagttcgatc
    12541 tggcgggcga ccgcgcgggc cagcatcacg cgctccagat ggcgctgacg atgttcgacc
    12601 gcgcgcgctt caccggcaag gggcgcgagg ccgccgtctt ccgcgactgg ctcgccgcgc
    12661 gcctctcgga agccgacctc gaagcgacgg acgacgcgtt agttgatggc gacgaagaag
    12721 cgggctgaag cgcgttcgca tgggcctaca cacttgagcc tctgcgccac tgccgtgaac
    12781 tgacggagtc cgttgcaaaa ctgtaggggc aggccaagtc cgcggccctt tacctacgga
    12841 agcaattaac atggatgggc aggatgagta ggatgaagaa agcgctgatt tcagatttga
    12901 gatttcaaat ttcaaatctc ttatcctgat tatcctgtcc atccatgtaa atttcttccg
    12961 taggttaagg gccgcggaca agccctgccc ctacagtcat cacccgcgtt gagttttgaa
    13021 acgaactctt agctttcaca caagcttgaa ggcgagcgac tcccaccttg accgataaga
    13081 ccaccgaaca ggcgactgcg gcgctccccc gcgagatgaa cctgcgcgac ctcgtgctct
    13141 tcaacctcgc cgcgctcatc ggcctcacat gggtctccac ggccgccagg gccggcccca
    13201 gcgccatcac gctctggctc gtcgccgccc tcctcttctt catcccgcag ggtctcgccg
    13261 tcgtcgaact ctcctctcac ttccccgacg aaggcggcat ctacgcctgg acgaagcgcc
    13321 agttcggcgt gggccacggc ttcctctgcg gctggtgcta ctggatcaat aacgtcctct
    13381 tctacccctc ggtgctcttc tccatcgcgt cgctcaccgc ctacgccatc gaccccggcg
    13441 gcgacttaag cgaccgctgg acgttcatcc tccccttcac gctcatcatg ctgtgggccg
    13501 ccgccacgct caacgccgtc ggcgtcggcc gcggcaagtg gctccagaac tgcggaggcg
    13561 cgagcaccta cgtcgccggc gccgtcctca tcgccttcgg cctctacggc gtcctcaccg
    13621 cggagccggc caacgccttc accctcgaat ctttgcgccc gagtttcgcc gacgtgtcct
    13681 cgctcaactt ctgggcgacc atcgccttcg ccttcgccgg ccttgagctg tcatcgacga
    13741 tgggcagcga gatcaaggag ccgcgccgca acctcccgct ctcgatctac atcgccgcgc
    13801 cgctcgccgc cctgctctac atcctcggca cgggcgcggt gctgtggtat ctgccggccg
    13861 gtgagatcga gatcatcccg gcgccgtttc aggcgattgc ggtgggggcc gctcgtctga
    13921 gcgcgtcgct gtggtggctg gcgcccgccg tggccgcgct cgccgccttc ggccggctcg
    13981 gcggattggg cgcgtggctg acggggtcgg cgcgcgtcgc cttcaccgtc ggactcgacc
    14041 gctacttccc cgccgccttc ggccgcgtcc acccgcgctg gcgcacgccc cacgtcgcca
    14101 tcttcgtgca ggcggccatc gccacgctct tcgtcttctt cgccgtgttg ggccggggga
    14161 cgaccgtcga gcgcgctttc ctcgtcctca tcgacatgtc gctgctgatc tacttcatcc
    14221 cctacgttta ccttttcctc tgcttcatcc tccacctgcg ccgcacgccc ccggagcgcg
    14281 tccaaatccc cggcggacgc atggcgggga tgcttctcgg cgtctgcggt ttgttgacga
    14341 ccctgttcgc gatggccgtc gccctcatcc cgccgcccga tacgccctcg cccggcatgt
    14401 tcattctcaa ggtcggcggc ggcagcctcc tcatcgtcct cgtgagcggc ctcatctact
    14461 ggcgcgcgcg gcacagacgc gtcgaggcga actaggcaga gtagcgggcg cgctcccggc
    14521 aagtgcttga aatgcctgta gacgagactg acgaactcac actgagcgac gaacgcacgg
    14581 gcgcgattgc cttcagccgc ggcgcgcggg tcgcgttggt gcttgtcgtc tgtctgtacg
    14641 tcgcggcgcg cgtgtggcgg ctgacggatt cgtgtctgtg gttcgacgaa atcttcggcg
    14701 tccacgcggc gcggcactcg tggggccgcc tcctcggctt cgcggcggcc gacctcatcc
    14761 acccgccgct cttctacgcg ctgctgaaac tgtggatcaa tgtgggcggc gagtcgctgc
    14821 cgtggctgag gctgcttagc gtcctcctct caatcgcgtc ggtcgcgccc ctcgtgcttc
    14881 tctgccgcga gctgcggctc ggcgcgtggg agacgaacct cgcgctcgcg ctcgcggccg
    14941 tgaacggcta tctcatcaag tacgcgcagg aggtgaggat gtacagcctg ctgctgctcc
    15001 tcacgctctg ctcgctgtgg ctgctcgcgc gcttcttcaa cgcttcggac gaacgccgct
    15061 cgcgtcagac tttagtcgcg ctcgccgccg gcaacctcct gctcgtctac acgcactact
    15121 acggctggct cgtcgtcgcg tcggaggcgg cgctgctcct gctgcttcgg cgcgggcgaa
    15181 agcgcgcgag gctggcgccg tttctgttga cggtcgcgct ggcggccgtc gcctatctcc
    15241 cgtgggtcta cgcgctgtcg cagcagcgcg gcgagcaggg ccgcgctctg gcgcagaaca
    15301 tcgggtgggt cgagcggccc gcccccactg atgtcttgca gttcctcgcg ctgctccatg
    15361 agcctttcta cttccgacga agctcggccg acccggtcta cacgaagctc acggcgccgg
    15421 cgggattgct gttgttcggc gtgcccatcc tgcttttgct gtggcgcacg gcgaagcgcg
    15481 cgcgcgccgg ggacggggcg cgccggggcg ctctgtgttg gctgcttgtc ttctcctttc
    15541 tgccgctcct gctcgcgttc gccctgagtt gggcgctgcc gcactcgatc tggggcacgc
    15601 gccacctgat cgtcgtcgcc gcgccctatc tcgtcatggc cgccgcagcg gtcgtgcgac
    15661 tgcgccccga gtgggcgcgc gccgcggtgc tcgtcgtgct gggctgctgg gtcttctggg
    15721 gcgcgctcgg cgcgctcgtg cggcgcgagg ccgcgcccgt ctggtgcgcg tgggagggtc
    15781 acgcggcgcg aatgaggagg gccgagacag cggcgcccgc gggcgtcgtg agcgtctacg
    15841 ctttcgaaga cctcgtcgcc tatcatctct ggttcgcact tgaaggagcc ggcgacgggc
    15901 gcttccgcgt ctcggtcgtc aagggcgtgc ccggtcttgc ggaagacccg gcctactttc
    15961 tgccgcgcgc cttcgacggc gtcggcgtgg gcgacgcctc ggccctcgaa ggcgcgcgca
    16021 tctgggtggc cttccgcgcc gcggcgtggg acgagacacg agcgccgctg aagactgttt
    16081 tggaaagagg ctaccgcgtt gaagaggttt acgagacgag cgcgcaggga cagcgagcct
    16141 tcatggtctc gttgaggaag gattgactgg cgcgcacagt accgcgagcg gtagcgagcg
    16201 ggtctgacgc acgcgagacg agtcactcat cgcaggcaca cccgctcgct accgctcgcg
    16261 gtactgttgt catgctctca ttgaaatcaa tcctgttgga gcgcaatgga ttccgagaaa
    16321 cgtaaacatc taatcttgct cggcgtcggc ctcgccgccc tcgccgcctc ggccgtcagg
    16381 ccgttcgact ggctgacgtg ggtgctcgaa gtagcgcccg ccatcctcgg cctcgcgatc
    16441 atcctcttcg tctaccggcg cttcaggctc accaccttcg cctacgtctg cgtcctcatc
    16501 cacgcgctca tcctcttcct cggcgcgcac tactcttacg cgaaggcgcc ccccggcttc
    16561 tgggtgcaag acctcttcga cctctcccgc aaccactacg accgcctcgg ccacttcgcg
    16621 caagggttct tccccgccat ctacacccgc gaaatcttcc tccgcctccg cgtcgtcaag
    16681 cggggctggc tcttcttcat cgtgacggcc gtgtgcctcg ggatcagcgc cttctacgag
    16741 tttacggagt ggtgggcggc gctcgcgggc ggcgactcgg ccgaggcgtt cctcggcacg
    16801 cagggcgatc agtgggacac gcagtgggac atgtttctct gcgccgtcgg cgccgtcctc
    16861 tcgcaactcc tcctcggacg cgtccacgac cgcgccgtcg cccacatgag cgctgtcaac
    16921 gccccggaag agcggtgact ctcgaaacgc gcgtccccac gtcgccgccg gctcgtccga
    16981 cgaaaacccg cgcgcggctt actcggtcgg agccggccgt ccgtctggtt gtagttgggc
    17041 acgccgccac cttcgactta tgatagattg ggcaaaattg gggggaggag aaagattatg
    17101 gagagtgcgg atagagtgcg tgctaatact tctgaagaag ttaactgccg gatcgaccgc
    17161 gagctagaca cccgcgtgcg ggagtacgcc cacagaagcc ctgacgacat cacccgccgc
    17221 atcagcgaac ttgaccgcga gtgggacatg gagcggctgc tcgaaaccaa cgcctcgaca
    17281 gtcgctttca ccggactcgt gctcggagtg acacacagta agaagtggct gatcgtgccg
    17341 ggcatagttt taccgttcct gtttcaacat gccgtgcagg gttggtgtcc gcccgtcccc
    17401 atcctgcgcc ggctcggtgt ccgcacccgc gaagagagag accgagaaaa atatgcgctg
    17461 aaagtcttgc gcggcgattt cgataatgcc gactcactgc cgcgtgcgga agcggcgtta
    17521 caatcagtga aagcttaagg gcgtcggcga gtaggatgag aggaggatga cggcggtgca
    17581 tgtccgagca cttagtacag acgaagagct gcggcgttat gttgacttcg gtcaggaagt
    17641 ctatcgccag aaccccaact gggtgccgcc tgacgcccat catctgacgg aactgctgag
    17701 cggccggagc gccttcggcg cgcagtcaca ggtgcaagcc ttctgggtcg aagagggcga
    17761 gcgcgtgttg gccgtggtca cggccatcag ggatgaagtg tatacccggc actggaacga
    17821 acagacgggg cacctcctgt ttttcgaggc cctgccccgt tgcgacgagg cggtcgagtc
    17881 gctcttccga gcggcgggtg attggctgcg ggagcgcggc tgccgggcgg cgcgtctgtc
    17941 ttttctggcg ggctggcaga tgcccctgac catcgacgcc tacgacgacg tgccgaccat
    18001 cttccacacc tacaatcctg cctactatca cagctacatc aagaacgccg gcttcagaac
    18061 cgaatacggc gtggtgcaat atcaaatcca gttcaccccc gaactggctg ggcgctaccg
    18121 cgagatgatc gagcgcgtca cgaactcggg cgtctccctc cggtcgtggg acttcgaccg
    18181 cccggaagag gagaaccaga ccttcacgga catcgccaat gagaccttcc gggcacactg
    18241 gggcttcaac ccgatgccgg catccgccct acgtgggctg acggtcgagt tgaaggattt
    18301 tttagtcgcc gacttcatcg tgtttgccga ggctgaggga cagaccatcg gcgccgtcta
    18361 ctcgctgccg gacttgaatc aggcgttcca ccggatgaga ggcaaggcaa tcgaagaaaa
    18421 cctccccgag cttcagcagc gccttcagga catagatcac ggggtgcttc tgatcatcgg
    18481 ggtgaaggag agtcaccgtg ggcgcggcgt caatctggcg ctggccgcaa aaagttacct
    18541 cgcgatgatc gaacgcggct acaagaccgg cagctacacc gtcgtgctcg acgacaactg
    18601 gccctcccgt cgcacggccg aaaaactggg cgcccgcgtc accagaaatt tcaacatcta
    18661 ccgcaaagag ttaacgcggc cgtccgcgtg agagacgttt gccaacgcga gcctgacact
    18721 tgcctttcat catcattagc tggctcaatc cgagtcgctc atctttgggc ggtgggttac
    18781 aaatgcgggc gcgagatgtc ttacgttagt caaagaaaaa ggagataagc acaatggcgg
    18841 atcagaaaga caaggcgcag gagaaaaagc ggcaggaaga atctgccggc agtgagcaga
    18901 acgatgaaca agcgagcggc cacggcagtg gcggtggcaa catgggcgcg ggaaagtctg
    18961 gcaaggaagg catgggcagc gaaaagccga gcgatgatga gaggatgggc agccacaagc
    19021 ccggcggctc cggttcacag taataaattc aaacgtgcgt gtttacggaa tgctcgcgag
    19081 cgagtggcga gataaagaca gatcgtcgcg gcggcccaat tcaggagtta tgccctgcgg
    19141 tagacttcgc gcccgtcgct cgtgagttcg acttccacct tgcggtcggt gtgggcgagg
    19201 tcgaggtggg cgaccgactc ggagacggag aggaagcggt gtacgtcgtc cgtgtcgggg
    19261 aagaggccgc gggcgacctc ccacccggtc gcgccctcct tcgggatgag cttcatcacg
    19321 tccgactgtc gctcgcgcac ggcgcgcagg tagcggttga agagttcttc gtagtcgtgg
    19381 accggctcgc cgtggccgcc gaggacgagt gtggggtgga gggcgcgcag gcgcgacaga
    19441 ctcaccagat actcttcgag cgagcggaag cgccggccgg ggtcgatggg gtcgggcgag
    19501 aggatggggt tcggcgtgat gcgcttgagc acacagtcgc cggcgatgat ggtgcggtcg
    19561 gcctcgcgga agaaggagca ggagccgggc gtgtggccgg gcgtgtggac gacgcgcagc
    19621 gagccgttgg caaactccaa atcctcttcg tcgtacaact ccgagtgctc gtcgtcttcg
    19681 agcgcgtcgg cgagctggcg cacctgctcg tagacgcggc gcatcccctc gatctcgtct
    19741 tcggggacgc cggcgcgcac cagaagcgcc cggtgctcct cgtactcaag cctgccgatg
    19801 cggtgccccg tctcccagcc gtgtaccaga acctcggcgt ccttcgcctc gtcgcgcaaa
    19861 ctcttcgcca gcccgcagtg atcctcgtgc gcgtgcgtca gcacgatgcg ccggatgtcc
    19921 gacgggcgga cgcgctcgcg ccgcaagccg tcctttaagg cttcgagcgc ctccttggtc
    19981 ttcggccccg tgtcgatgag cgtcagcggc tcggcgacca cgaggtagca gttgaccggc
    20041 ccgacgtaga agggcgtggg gacgacgatg ggaacaattc tcataaggca aagggcaaag
    20101 ggtaaagggg aaggggtaaa gggtaaagcg gagcggcgac gctaaagacg accgtcttcc
    20161 tttacccttt accccttccc cctttcccgt cgtcagtaga cgtacttatt ggctgtgatc
    20221 atcgcgtcgg cgattctttg tcgcgcggcg acggtgttca tgggcgtgtt cttggtgaag
    20281 cggcgcaggg cggcgaggag ggtgcgtagc tcatcgccct cggccatcgc ggccagcgtc
    20341 tctttggcgc gcgtttcgat gcgcgcggcg gccccctcgc agaagacgcg cgtgatgtcg
    20401 aggtaacgcg ccgaggcgtc ttcgcccttc gcgtccgtca tcttccgcgt gcggaggatg
    20461 gccgactcca tcgcgtaaac gtccatgatg atgtcggcga cgccgagcag aacctcctgc
    20521 tggtcggcga gcgccatcat gtacttctgc gcggccgtgc ccaagaccat cagcgcgacc
    20581 ttcttcgcgt tgcgcgcgag ccgcagctcg ttcgagagcg gcccctcgtc ttcgtccatg
    20641 cctgacatgg agggcgagag cacttcgtcc atgagggcct gcgcggccgg gagcagggcg
    20701 agctgcccct tcatcgcctt cttcatgagc atgccgggga tgagcatgcg gttgatctcg
    20761 ttggtgccct cgaagatgcg gttgatacgc gagtcgcggt aggcgcgctc ggccgggtag
    20821 tcggccgaat agccgtagcc gccgaagatt tgcaccatct cgtcggcgac gaagtcgcag
    20881 tactcggaca gcgccacctt gatgatcgag cactcggtgg cgtactcctc gatggtgcgc
    20941 cccttgtcca acatgctggc gtcggcgccg agggcggcca ggccggcgtc taagaggccg
    21001 agcgtgcggt agttcatcga ctcgccgacc cacgtccgca ccgccatctc gccgagcttg
    21061 gccttgatgg cgccgaagga ggagatgggc ttgccgaact ggtgccgctc gttggcgtag
    21121 cgcacggcct cctgcgtcat cagcttcatg ccgccgccgc acatcgcgcc gagtttgaag
    21181 cggccgatgt tgagcgtgtt gagagcgatc ttgccgccca tgccgacttc gccgaggaga
    21241 ttcgagacgg gcgtctttac gtcggagagg acgagggccg tcgtcgaaga ccccttgatg
    21301 cccatcttgt gctcctcggc gccgttcgtt agtccctcct gccgctcgac gatgaagccg
    21361 gaaaactttt cgccgtccac cttggcgaag acgacaaaga tgtcggcgaa gccgccgttg
    21421 gtgatccaca tcttctcgcc gttgaggatg tagtgtgtgc cgtcgtccga gaggcgcgcg
    21481 gtcgctttcg cggcgagggc gtcggagccg gagccggcct cggtgagcgc gtaggccgtg
    21541 accagctcgc ccgagacgat cttcggcaga tacttctgct ttgcctcctc gctgccgaag
    21601 tagacgatgg gcaacaggcc gatgccgctc tgcgcgccca tcgtggtggc gaaggaggcg
    21661 gagcggccga gcagttcgcc gatgaccgcg ccggtggtct gatccatgcc gaggccgcca
    21721 taagcctcgg ggacggtcgc gccgaccaga cccagctccg cgcccttgcg gacgagttcg
    21781 cgcgccagct tccagtcgtg cttctcaagc tccgctgtgc gcgggcgcac ctcgttgtcc
    21841 atgaactgcc gggtcgtctc ggcgatcatg cggtgctcct cggtgaagtc ctcgggcgta
    21901 aagacctcgg cgggcgcgcg ctcctcaatc agaaaactcc caccggcgaa aacttttttc
    21961 tcttcggcta ctgcggacat agtttgctcc tttcagtcgc aagtaaatcg tgaatcgtaa
    22021 atcgtaaata gttcgtcttt cgttaaccgt gaatctttaa agggatgagg ctttgtgcgg
    22081 gacgcctcga ccattcgatt cacgatttac gattcacgat ttacagtctt tcaaatattc
    22141 ccgcggcgcc catgccgccg ccgacgcaca tcgtgatcat ggcgtagcgc ccctcgcggc
    22201 gttcgagttc gcgcagggcc gtggccgtca gcttcgcgcc ggtgcagccc agagggtgac
    22261 cgagggcgat ggcgccgccg ttgacgttta cgcgctcagg gtcgaggacg agcgtgcgga
    22321 tgacggcgag cgattgcgcg gcgaaggctt cgttgagttc gatcacgtcg atgtcttcga
    22381 gacgcaggcc ggcgagcttt agaactttgg ggatggcgtg gacggggccg atgcccatct
    22441 cctcgggcgg gcagccggcc gtggcgtagg cgacgaaacg cgccagcggt tttaagctca
    22501 actgccgcgc acgctcgtcc gagacgatga cgacggccgc cgcgccgtcc gacatctgcg
    22561 acgcgttgcc ggcggtgatc gtgccgccgg tgtgaaacgc cggccggagt ttggcgagtc
    22621 cttcctccga agtgtcgcgc cgcacgccct cgtctttatc gaagctcact tcgcgcgtgg
    22681 tcttcttccc cttgtcgttc aactcctcga aggtcacgct gagcggcacg atctcgtcgt
    22741 cgaacttgcc ggcgtcctgc gcggccgccg cgcggcggtg tgagagggtc gcgaaggcgt
    22801 cctgctcttc gcgcgagact tcgtacttgc gtgcgacgtt ctcggtggcg aggcccatgt
    22861 tgaggtagaa gtccggatag tgttcgacga ggtgcgggtt cgggcggatg atgtggccgc
    22921 ccattggaat cagagacatc gtctccagcc cgccggcgac gcagacctcg gccgctcccg
    22981 tcctgatgcg gtcggccgca atcgcgattg actggagacc ggaggagcag aagcggttga
    23041 tcgtcatcgc gctcgtctcg acgggcagcc ctgcgcgcac ggcggccgcg cgcgcgacgt
    23101 tcatgccctg ctcggcctcc ggcatcgcgc agcccatcac cacgtcctcg acctccgccg
    23161 cttcaacgcc cccggcgcgc gccagtgctt ctttaatcac cgccgcgccc atctcgtcgg
    23221 gccgagtgtt cctgagcgtc cccttcggcg ccttgcccac ggccgttcgc actgcggaaa
    23281 caattactgc gtctctcatg gttcactccg tactgagttc aaagttcaag gttcaaagtt
    23341 caaagtcaaa gactggttgt tcctttgaac tttgaacttt gaactaattc ctgagcggct
    23401 tgcccgtctt gagcatgtgg gcgatgcgct cctgcgtggg gcgctggccg cacaggctga
    23461 ggaacgcttc gcgctcaagg tcgagcagat attgttcgct gacgcgtgtc gggtggttga
    23521 ggtcgccgcc cgtgacgacg cgcgcgagtt tttcgccgat aagagcgtcg tagtccgaga
    23581 tgaagccgcc gcgcttcatc tggtggatgc cgagcttgag cgtggagagg gcggagagtc
    23641 cgggcgcgaa aacgtctgta cgcggctgcg ggcgcgtgta gccggactgg tggagtgcga
    23701 gcacttcctg tttggcgtcg gcgatgagac gctccgagtt catcgagtag gagtcttcgt
    23761 cgcggaggaa accgagtttg cgcgcctcct cggcggaggt ggccaccttc gccatcgcca
    23821 tcgtctcgaa ggcgcgtttg aagaacggga acgggtcggc ttcctctgcg ccggggggga
    23881 tggagtcgcc cgcgcgcatg gccatctcct tcgtcccgcc gcccgccggg atgaggccga
    23941 cgccgacctc gaccagcccg atgtaggtct cggcggcggc gcgcacgcgg tcggcgtgca
    24001 gagtcatctc gcacccgccg ccgaaggtca tcccgaaggg cgcgacgacg accggcttcg
    24061 ccgagtagcg caagctcatc gtcgcctgct ggaaggcgcg cacaccgagg tcgatctcct
    24121 cccactcctc ctcctgcgcg ccgaggagga cgagcatcag gttggcgccg acggagtagt
    24181 tctggccctg attgccaatc acgagtccga cgaaatttcg ttcaacttcc gcgagtgccg
    24241 tcttgaccat ctggagcgtg tcgccgccga tggagttcat cttcgaatga aactccagac
    24301 aggccacgcc gtcgccgatg tcgatgagcg acgcgccggc gtttttcttg acgacgcccg
    24361 tgcggtcttt aaccgacttc aaaacgacga cgcccggctg ctccgccacg gggacgtaag
    24421 cgcccgtagc gaagtcgaag taagactgct ggccgccctc cgacttgtag aaagatttcg
    24481 cgccggcttc gagcatccgc cggacgttct caggaacctt gcgcccctcc tcttcgagcc
    24541 gcgcgacgga tttctccacg ccgatggcgt cccacgcctc gaagacgccc aactcccagt
    24601 tgaagcccca cttcatcgcg cgatcaactt ccagcacggt gtcagcaatc tcagggatgc
    24661 ggttggcggc gtagacaaac gtcttcgaga tcgtcttcca cagaaatgcg ccggcgcgct
    24721 ccttgcccca cgtaagcgtc cgcaaacgct cgcgcgtgtc ctcgatgttc ttggcggcgt
    24781 cgagcgccgg gagttttacc ttctcggcgg ggcggtattc gagcgtggcc acgtcgagcg
    24841 tccagatttc ctgcttccca ccctcgccct tctgccggcg gtagaagccg cccttggtct
    24901 tgttccccag aagcccccgc tcgaccatgc cgcgcgcgaa gtcgggcgcg agataggcgt
    24961 cgcggtcttc gtcttcgggg acggcctcgt agagattttt cgcgacgtgc ataaacacgt
    25021 cgaggccgac gaggtcgaag agacgga
//
LOCUS       DASVES010000506         9372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_113773, whole genome shotgun sequence.
ACCESSION   DASVES010000506 DASVES010000000
VERSION     DASVES010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113773"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1447
                     /locus_tag="VF240_21380"
     CDS             203..1447
                     /locus_tag="VF240_21380"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7177826.1"
                     /translation="MTAVEKMTSGALGWEVLAEEGEARAGVLQTRRGRIETPVFMPVG
                     TLGTVKGVRFEALESELEARIILSNTYHLWLRPGPETIRACGGLHRFTGWPRALLTDS
                     GGFQVWSLGHLRKLREEGVEFRSHVDGRACFLSPEVSMEVQAALGSDIVMAFDECAPG
                     EATHDEARVSMELTARWARRSRTRFDELQREGLDAGRVASTSDDDEREATDDYDAASA
                     RLSGAQALFGIVQGATHLDLRRESLERTVDVGFDGYAIGGLSVGEEKPVMYEVVESVA
                     PLMPRDRPRYLMGVGTPEDLLECVARGVDMFDCVLPTRNGRNGQAYTSRGRLNIRNAR
                     WAADPRPLDEECACAVCRRHSRAYLRHLHATGEMLAAILLTHHNLAFYLDTMRRVRQS
                     IRSGQFTRFRREFIGKLNSGAG"
     gene            1576..1899
                     /gene="yajC"
                     /locus_tag="VF240_21385"
     CDS             1576..1899
                     /gene="yajC"
                     /locus_tag="VF240_21385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7177827.1"
                     /translation="MSLLMLQPGQESGGGLLPFLVQMMPFILIFFVFYFLIIVPQRKR
                     QRALQETISALKAGDRIVTSGGIIATVTAVRDASLLVRSADKSMLEISRAAVAGLHGE
                     EEKKG"
     gene            2002..3696
                     /gene="secD"
                     /locus_tag="VF240_21390"
     CDS             2002..3696
                     /gene="secD"
                     /locus_tag="VF240_21390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7177828.1"
                     /translation="MKKNLLHRVIIILVVTLVALWIVIGPRRWPPTLADFTPQGINNT
                     LRNNIRLGLDLRGGSHLVMQVQVADYLKRLTENVAVGLQNSAKEQQFAISNATPQVEG
                     DDYRIIVEAGEGVDVQKLKDELPKRANDFDPSIWTASVDGRRVVWEMSDAAKTVLSSQ
                     AVAQAKQIIDTRINAVGVAEPNLSDHGAQGSHQILLQMPGERDPERIKKTLTAESRLE
                     LVHVISPQGMLQKYPTKEAALQSIGGAETQTRKVYPYVERDAPVEGEPEPAQKPEPEW
                     VVVETPNVIEGADLRDARSSQGDGGGDIYQINFDLKPRGAEKFGRWTAANIGNYMGVV
                     LDDQVKSVATIQSQINDSGRITGSFTKNQAEDLSLTLRSGALPAKLVYLEERTVGPSL
                     GEDSIRAGVRASVAGLVFVIIFMVLYYRGAGINAVVALLLNMLITLAAMIWPLDASLT
                     LPGIAGLILSVGMTVDTNVLIFERIREELLTGKTVSSAVDQGFSRAFVTIIDTHVTTI
                     VSSLFLFVFGSGPVRGFAATLIIGLVANLFSAVYVSRTIFMWVLSRKGKRAESLSI"
     gene            3796..4869
                     /gene="secF"
                     /locus_tag="VF240_21395"
     CDS             3796..4869
                     /gene="secF"
                     /locus_tag="VF240_21395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7177829.1"
                     /translation="MLRILHDVKVDWLALRKPLLMFSILLLLAGLVSAIGRWATPGGT
                     EPFNLGVDFKGGTLVTVDFKSPPSLDDIRNSLSGVGMGDAVIQPALDKQGRFLIKVAQ
                     QGTGEEAQSKAEVDDGAAKVGEALAGFGQRVDRATGEGDWDIVSKDAVGPVAGRQLRN
                     QAITVTLLALVGILLYIAFRYEWTYGASAVITVFHDVLVTLAFFTVFQWEVNLTTIAA
                     LLTLVGFSVNDTIVTFDRVRENLRLHRRATLYDTTNEAINQTLSRTVITAGLVFLSVL
                     ALVLFGGEVLRSFSLALFIGVIIGTYSTIAISNPLMVWWQQRLERAGRGGAKETLPRP
                     EGGPRQTRTTTAPAKRRAGAAAR"
     gene            5341..6162
                     /locus_tag="VF240_21400"
     CDS             5341..6162
                     /locus_tag="VF240_21400"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7177830.1"
                     /translation="MFENLVESGSHKDDLARKGSFLLGTLVVYGIVFVAVGILSVYLY
                     NANLPDTSLELTTLVAPVPAAPEPEAKPEQKEEQKPEAKAEPQVSMRQEISMIMPPDM
                     SKNREVAKAETKEIARNVPVVIGNRDVDAAPSAMTSGPIGPAVSGGGGGGGGGTGPVV
                     KSDDDAPPPPPKPTPAPKKNVTISGGVLNGKAVSKPAPAYPAIAKAARASGTVTVQIT
                     VDESGRVVSATPVSGHPLLQQAAASAVRQWRFTPTLLSGQPVKVTGVVTVNFNLQ"
     gene            6323..7087
                     /locus_tag="VF240_21405"
     CDS             6323..7087
                     /locus_tag="VF240_21405"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7177831.1"
                     /translation="MNVVGSKALSFLVFLWQEGGAGGETTMQEAFTISEMIKNLGWVA
                     LTVIVILLIMSMYSIAIMVERFLTYSAAKKQSREFAPRVAQALKNDRIEEAINISDKH
                     KKSHLAMVVNAGLQEFRAHEQSADISGEEIEASKRALQRAIAIKSAEFKRGLSGLATI
                     GSTAPFVGLFGTVFGIINAFTGMKSAESAGIGAVAGGIAEALLTTALGLAVAVPAVWL
                     FNYFTGKVDGFIVEMDNSASELIDYFLKNRTRQLKS"
     gene            7218..7685
                     /locus_tag="VF240_21410"
     CDS             7218..7685
                     /locus_tag="VF240_21410"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7177832.1"
                     /translation="MSMSTGSGSGGLNSDINVTPMVDIMLVLLIIFMVITPFLQQGVS
                     VALPRDMKNPDEDKAIIKESSVVVAVTADGKLFLGKKPIEKPDLKAEIDKKMEGKAAD
                     ERIVYIRSDIGADYGKVVDTINTIRESGIDQIGLVADKKKGGDAAPAAAAPAS"
     gene            7761..8276
                     /locus_tag="VF240_21415"
     CDS             7761..8276
                     /locus_tag="VF240_21415"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7177833.1"
                     /translation="MSSSGGSSSGSTSAQPNINVTPLIDILLVLLIIFMVITPLKPAR
                     FKTLVPEKSDEITEAAKVSPRTLSITVGKDMSVKLVRGADPVAEGTVNDMGGLAAWLS
                     QEFKGRREGAVWKAGFENRIDLPPDDRIEKTVFIKAPQSFKYGEVVKVIDAVKGAGAN
                     PVGLQTEMLDP"
     gene            8388..9257
                     /locus_tag="VF240_21420"
     CDS             8388..9257
                     /locus_tag="VF240_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177834.1"
                     /translation="MKLSLNRLVLLLMVALTAASASGCGAVNRIRAKNALNEGARAYK
                     DGRFDEAEQKFRSARELDPDQKNAPLFIARAVHQQYKPGVDTPENKEKADRAVEAYKQ
                     VLAAPGIEEKHREDAYSAIAYIYRQMREEKKEEEWLTQRANSGDAPPAKRSDAYTILA
                     SKQWDCAYNINEQKESKKTIDKGDKGIVVEYVKPKNPDDFNKAMGCVDRGLDLVNRAI
                     EQNKDNPNAWSYKTNLLRERAKLAQMDGKPEDKARFDSEANAAEQEQKRLNAAANQKK
                     ADEDAKKTPPAAS"
BASE COUNT         1745 a         3126 c         2928 g         1573 t
ORIGIN      
        1 acatggatga gcaggatggg gaggatagag gatttgaaat ctgaaatctc aaatctctaa
       61 tcagcacttt cttcatcctg cttatcctgc ccatccatgt tgattcctac gtagacgggc
      121 agcggacttg tccctgcccc tacatccgtg gctcgcgttg agggcttgaa ccttactact
      181 gttactgttg tgttccgaga agatgactgc tgttgagaag atgacgagcg gcgccctcgg
      241 ctgggaggtt ctggccgaag agggagaggc gcgcgccggc gttctgcaaa cccgcagggg
      301 ccgcatcgag acgcccgtct tcatgcccgt ggggacgctc ggaaccgtca agggcgtgcg
      361 cttcgaggcg ctggagtcgg aactcgaagc gcgcatcatc ctctccaaca cctatcacct
      421 ctggctgcgg cccggccccg agaccatccg cgcttgcggc gggctgcacc gcttcaccgg
      481 ctggccccgc gcgctgctca ctgactccgg cggttttcag gtgtggagcc tcggccacct
      541 gcgcaagctc agggaggagg gcgtcgagtt ccgctcacac gttgacggcc gcgcctgctt
      601 cctctcgccc gaggtctcga tggaggtgca ggccgcgctc ggctccgaca tcgtgatggc
      661 cttcgacgag tgcgcgcccg gcgaggccac gcacgacgag gcgcgcgtct cgatggagct
      721 gaccgcgcgc tgggcgcgcc gctcgcgcac gcgcttcgac gagctgcaac gcgagggctt
      781 ggacgcgggc cgagtcgcct ccacatccga cgacgacgaa agggaagcga cagacgacta
      841 cgacgccgca tcggcccggc tcagcggcgc gcaggcgctc ttcggcatcg tgcagggcgc
      901 gacgcatctc gacctgcgtc gcgagagtct ggagcgcacg gtcgatgtgg gcttcgacgg
      961 ctacgccatc gggggcctga gcgtcggcga ggagaagccc gtgatgtacg aagtcgtcga
     1021 atccgtcgcc ccgctcatgc cgcgcgaccg gccgcggtat ctgatgggcg tgggcacgcc
     1081 cgaagacctt ttggagtgcg tcgcgcgcgg cgtcgatatg ttcgactgcg tgctccccac
     1141 cagaaacggc cggaacggtc aggcatacac ctcgcgcggg cggctcaaca tcagaaacgc
     1201 gcgctgggcc gccgaccccc ggccgctcga cgaggagtgc gcgtgcgccg tctgccgccg
     1261 acactcgcgc gcctatctgc gtcacctcca cgcgacgggc gaaatgctcg ccgccatcct
     1321 ccttacgcat cacaatctgg ctttctacct tgacaccatg cggcgcgtca ggcaatctat
     1381 ccggtctggt caattcacgc gttttcggcg ggagtttata gggaaactca actccggcgc
     1441 cgggtgagcc gaggggccgt ttgcggcgct cggacgcgac cgcgaaggat tgcgccgggg
     1501 ccgcgttcgt taaggccgcg cccgtccatg aagggcgcac gaaagaatct cgcaaggtag
     1561 tcacggagga atcacttgtc actgttgatg ctccagccgg ggcaggagag cggcggcgga
     1621 ctgttgcctt ttctcgtcca gatgatgccg ttcatcctga tcttcttcgt cttctacttc
     1681 ctcatcatcg tcccgcagcg caagcggcag cgcgcccttc aggagaccat ctcggcgctc
     1741 aaggccggcg accgcatcgt caccagcggc ggcatcatcg ccaccgtcac ggccgtgcgc
     1801 gacgcgagct tgctcgtccg cagcgccgac aagtcgatgc tcgaaatctc ccgcgccgcc
     1861 gtcgccggcc tccacgggga agaggagaag aagggataag cggatagcca ttagctatta
     1921 gccattagct aatagctaaa actaaagctc aagctaaagc tcatcgccaa aagctcatcg
     1981 ctaatagcta acggcttcat catgaaaaag aatttgctcc atcgcgtcat catcatcctc
     2041 gtggtgacgc tcgtcgcgct ctggatcgtc atcggcccgc gccgctggcc gccaacgctg
     2101 gccgacttca ccccgcaggg catcaacaac acgctccgca acaacatccg ccttggcctc
     2161 gaccttcgcg gcggctccca cctcgtgatg caggtgcagg tggccgacta tctgaagcgg
     2221 cttacggaga acgtcgccgt cggactccag aactccgcca aggagcagca gttcgccatc
     2281 tcaaacgcca ccccgcaggt ggagggcgac gattaccgca tcatcgtcga agccggcgag
     2341 ggagttgacg tgcagaagct caaggacgag ctgcccaagc gcgccaacga cttcgacccg
     2401 agcatctgga cggcctcggt ggacggccgc cgcgtcgtct gggagatgag cgacgcggcg
     2461 aagaccgtgc tttcgagtca ggccgtcgcg caggccaagc agattatcga cacgcgcatc
     2521 aacgccgtcg gcgtcgccga gccgaacctg agcgaccacg gcgcgcaggg gtcgcaccaa
     2581 atcctgctcc agatgccggg cgagcgcgac cccgagcgca tcaagaagac cctgacggcc
     2641 gagtcgcgtc tcgaactcgt ccacgtcatc agcccgcagg ggatgctcca gaagtacccg
     2701 acgaaggagg ccgcgctcca gtccatcggc ggcgccgaga cgcagacgcg caaggtctac
     2761 ccatacgtcg agcgcgacgc gccggtcgag ggcgagccgg agccggcgca aaagccggag
     2821 ccggagtggg tcgtcgtcga gacgccgaac gtcatcgaag gcgccgacct gcgcgacgcg
     2881 cgctctagtc agggcgacgg cggcggcgac atctatcaga tcaacttcga cctcaagccc
     2941 cgcggcgccg agaagttcgg ccgctggacg gccgccaaca tcggcaacta catgggcgtc
     3001 gtcttagacg atcaggtgaa gtcggtcgcc accatccagt cgcagatcaa cgactcgggg
     3061 cgcatcaccg gcagcttcac gaagaatcag gccgaagacc tctcgctcac cctgcgctcg
     3121 ggcgcgctcc ccgccaagct cgtctatctg gaggagcgca ccgtcggccc gagcctcggc
     3181 gaagactcga tccgcgcggg cgtgcgcgcg tcggtcgccg gactcgtctt cgtcatcatc
     3241 ttcatggtgc tctactaccg cggcgccggc atcaacgccg tcgtcgcgct gctcctgaac
     3301 atgctcatca cgctggcggc catgatctgg cccctcgacg cgtcgctcac gctgcccggc
     3361 atcgccggcc tcatcctgag cgtcggcatg accgtggaca ccaacgtcct catcttcgag
     3421 cgcatccgcg aggaattgct cacggggaag actgtctcct cggccgtgga tcagggcttc
     3481 tctcgcgcct tcgtgacgat catcgacacg cacgtcacga ccatcgtctc atcgctcttc
     3541 ctctttgtct tcgggtcggg gccggtccgc ggcttcgccg ccacgctcat catcggcctg
     3601 gtcgctaatc tcttctcggc cgtctacgtc tcgcgcacga tcttcatgtg ggtcttgagc
     3661 cgcaagggca agcgcgccga gtcgctcagc atttgaaagc cgtgatcagt gatgagtgat
     3721 aagtgataag aataatcact tcctgctcat cacttatcac ttatcactta tcacttatca
     3781 ctcattacgg tctttatgct acgcatcctt cacgacgtaa aagttgactg gctcgccctg
     3841 cggaagccgt tgctgatgtt ctccatcctg ctgctgctgg cggggcttgt ctcggccatc
     3901 ggccgctggg cgacgccggg cggcaccgag cctttcaacc tcggcgtgga cttcaagggc
     3961 ggcacgctcg tcacggtcga tttcaagagt ccgccgtcgc tcgacgacat ccgcaattcc
     4021 ctgagcggcg tcggcatggg cgacgccgtc atccagccgg cgctcgacaa gcaggggcgc
     4081 ttcctcatca aggtggcgca gcagggcacg ggcgaggagg cgcagagtaa ggccgaggtg
     4141 gatgacggcg cggcgaaggt tggggaggcg ctcgccggct tcggccagcg ggttgaccgc
     4201 gcgacgggcg agggcgactg ggacatcgtc agcaaggacg ccgtcggccc cgtggcgggc
     4261 cggcagctcc gcaatcaggc catcaccgtc acgctcctgg ccctcgtcgg catcctcctc
     4321 tacatcgcct ttcgctacga gtggacatac ggggcgtcgg ccgtcatcac cgtcttccac
     4381 gacgtgctcg tcacgctggc cttcttcacc gtcttccagt gggaggtcaa cctgacgacg
     4441 attgcggcgc tcctgacgct cgtcggcttc tccgtcaacg acaccatcgt gaccttcgac
     4501 cgggtgcgcg agaacctgcg cctgcaccgc cgcgccacgc tctacgacac caccaacgaa
     4561 gccatcaacc agacgctctc gcgcacggtg attacagccg gcctcgtctt cctctcggtg
     4621 ctcgcgctcg tgctcttcgg cggcgaagtc ttacgcagct tctcgctcgc cctcttcatc
     4681 ggcgtgatca tcggcaccta ctcgaccatc gccatctcga acccgctcat ggtctggtgg
     4741 cagcaacgcc ttgagcgcgc cggccgcggg ggcgccaagg agactttgcc ccgccccgag
     4801 ggcggccccc gccagacccg cacgacgacc gcgcccgcca agcgcagggc cggcgccgcg
     4861 gcccggtaaa acaaggatga gggatgaggg atgagggatg aatgtgtctc cttattcatc
     4921 cctcatccct catccctcat ccctgctttt ctgcttgact tccggccgca cagcgacata
     4981 gactgagcgc gttgctggcc aatcgaatat ttacatcaag cacgcccgtt ctcgcctgag
     5041 gtgtgggacg gacgtttggg cgcgccccgc ttgcgggcga ccgcgccggc cggcttgatc
     5101 gctttcacgc actgaagcat cgggtgaggc gcttccctct tgggagcgtc ttcgttcgtc
     5161 aggcgtggtc gttagcggcc ggttctgcca ccccccactt tttttaagga atattttata
     5221 aatcgagcgg gcgtttttgt tgtaaagacg ttttagcacc aagaactacg tggttctcgg
     5281 atttcgtcac tacggcaccc ctgatccacc ggcaccggaa gagggaggat agacgcaacc
     5341 atgttcgaaa acttagttga gtccggttcg cacaaagacg accttgcgcg aaaaggctcg
     5401 ttcctgctgg gcacgctcgt cgtctacggc atcgtcttcg tcgccgtcgg cattctgagc
     5461 gtgtacctgt ataacgccaa tctgcccgac acgagcctcg agctgacgac gctggtcgcg
     5521 cccgtgccgg ccgcaccgga gccggaggcc aagcccgagc agaaggagga gcagaagccc
     5581 gaggccaagg ccgagccgca ggtctccatg cgccaggaaa tctcgatgat catgccgccc
     5641 gacatgtcca agaatcggga ggtcgccaag gcggagacca aagagattgc gcgcaacgtg
     5701 cccgtcgtga tcggcaaccg cgacgttgac gcggcgccct cagccatgac cagcggcccc
     5761 atcggcccgg ccgtcagcgg tggcggtggc ggcggcggcg gcggcaccgg cccggtggtg
     5821 aagtccgacg acgacgcccc gccgcctccg ccgaaaccga cgcccgcgcc caagaagaac
     5881 gtgacgatct ccggcggcgt cttaaacggc aaggccgtct cgaagcccgc gccggcctac
     5941 ccggccatcg ccaaggcggc gcgcgcctcc ggcaccgtca ccgtgcagat cacggtcgat
     6001 gagtcgggcc gcgtcgtatc ggccacgccg gtgagcggcc acccgctgtt gcagcaggcc
     6061 gcggcctcgg ccgtgcgcca gtggcggttt acgccgacgc tgctctcggg ccagccggtg
     6121 aaggtcacgg gtgtcgtcac cgtcaacttc aacctacagt agcggcgcgc acaggcgaga
     6181 ggcgcacgaa cgtttgcagg ttggcgcggc gctcccctcg gcggggccgc cgcgccccag
     6241 tgccacgcct ccctcgcagg cggcgcttct ggaattcagt ggagtttaaa aatctttgga
     6301 gggtcctatc gtgactattt tgatgaatgt tgtgggcagc aaggcgttaa gtttcctcgt
     6361 gttcctctgg caggaaggcg gcgccggcgg cgagaccacc atgcaggagg ccttcaccat
     6421 ctccgagatg atcaagaacc tcggctgggt ggcgctcacc gtcatcgtca tcctcttgat
     6481 catgtcgatg tactccatcg ccatcatggt cgagcggttc ctgacctact cggccgccaa
     6541 gaagcagtcg cgtgagttcg cgccgcgcgt cgcccaggcc ctgaagaacg accgcatcga
     6601 ggaagccatc aacatcagcg acaagcacaa gaagtcgcac ctcgcgatgg tcgtcaacgc
     6661 cggtcttcag gagttccgcg cgcacgaaca gtcagcggac atctccggcg aggagatcga
     6721 ggcctcaaag cgcgccctcc agcgggctat cgccattaag tcggccgagt tcaagcgcgg
     6781 cctttcgggt ctcgccacca tcggctcgac ggcgccgttc gtcggactct tcggcacggt
     6841 gttcggcatc atcaacgcct tcaccggcat gaagtcggcg gagtccgccg gtatcggcgc
     6901 ggtcgcgggc ggaatcgccg aggcgcttct gaccacggcg ctcggtctgg ccgtggcggt
     6961 gccggccgtg tggctcttca actacttcac gggcaaggtt gacggcttca tcgtcgagat
     7021 ggacaactcg gcgtcggaac tgatcgacta cttcctgaag aaccgcacgc ggcagttgaa
     7081 gtcgtagggc cgcggccgaa gtttcagggc ggccgagagc gcgccgccac accgttcatc
     7141 ttcgccgcgg cggcggggtt tcgggcggcg cgcgcgagcg gcggggcgtt caggttcgtt
     7201 aaggagattt gtaagtcatg tcaatgtcaa ccggttcagg tagcgggggg cttaactcgg
     7261 acatcaacgt cacgccgatg gtggacatca tgctggtgtt gctgatcatc ttcatggtca
     7321 tcacgccgtt cctccagcag ggcgtctcgg tggcgctgcc gcgcgacatg aagaacccgg
     7381 acgaggacaa ggcgatcatc aaggagtcgt cggtcgtcgt ggccgtcacg gcggacggca
     7441 agctcttcct cggcaagaag ccgattgaga agcccgacct caaggccgag atcgacaaga
     7501 agatggaagg caaggccgcc gacgagcgga tcgtctacat ccgcagcgac atcggcgccg
     7561 actacgggaa ggtcgttgac accatcaaca ctatccgcga gtcgggcatc gaccagatcg
     7621 gcctcgtcgc cgacaagaag aagggcggcg acgcggcccc ggccgccgcg gcccctgcct
     7681 cgtaacaggg cgacacaggg cgactatgcc cacgggcgcg cactgcgccc gcccctttgt
     7741 ggaggatttg agctatggga atgagttcca gtggcggcag tagtagcggc agcaccagcg
     7801 cgcagccgaa catcaacgtc acgcctttga tcgacatcct gctggtgctc ctgatcatct
     7861 tcatggtcat cacgccgctc aagccggcgc gcttcaagac gctcgtcccc gagaagagcg
     7921 acgagattac cgaggcggcg aaggtcagcc cgcgcaccct gtcgatcacc gtgggcaaag
     7981 acatgtcggt gaagctggtg cgcggcgccg acccggtggc cgaaggcacg gtcaacgaca
     8041 tgggcgggct tgccgcgtgg ctgtcgcagg agtttaaggg ccgccgggag ggcgccgtgt
     8101 ggaaggccgg cttcgagaac cgcattgacc tgccgcccga cgaccgcatc gagaagaccg
     8161 tcttcatcaa ggccccgcag tcgttcaagt acggcgaggt cgtcaaggtc atcgacgccg
     8221 tcaagggcgc gggggccaac cccgtgggcc tccagacgga gatgctcgac ccgtaaggcg
     8281 cgtgacccat aggcgtcggg ccgctcccgg ccggcgaccg caacaccgtt ggcgggacgc
     8341 gcgcgatatt tcgctccgaa cacaaccgga acttggggtt caagtcaatg aaactctctc
     8401 tcaacaggtt agtcctgctg ctcatggtcg ccctgacggc cgcgtccgcg tccggctgcg
     8461 gggccgtcaa ccgcatccgc gccaagaatg ccctcaacga gggcgcgcgc gcctacaagg
     8521 acgggcggtt cgacgaggcg gaacagaagt tcaggtccgc gcgcgaatta gaccccgacc
     8581 agaaaaacgc cccgctcttc atcgcccgcg ccgtccacca gcagtacaag ccgggcgtgg
     8641 acacgccgga gaacaaggag aaggccgacc gcgccgtcga ggcttacaag caggtcttgg
     8701 ctgcccccgg catcgaggag aagcaccgcg aggacgccta cagcgccatc gcctacatct
     8761 accgccagat gcgggaggag aagaaggagg aggagtggct gacgcagcgc gccaacagcg
     8821 gcgacgcgcc gcccgccaaa cgctccgatg cctacaccat cctcgccagc aagcagtggg
     8881 actgcgccta caacatcaac gagcagaagg aaagcaagaa gaccatcgac aagggcgaca
     8941 agggcatcgt cgtcgagtac gtcaagccca agaaccccga cgacttcaac aaggccatgg
     9001 ggtgtgttga ccgcggctta gacctcgtca accgggccat cgagcagaac aaggacaacc
     9061 ccaacgcctg gtcgtacaag accaacctgc tgcgtgagcg cgccaagctg gcgcagatgg
     9121 atggcaagcc cgaagacaag gcgcgcttcg attccgaggc caacgccgcc gagcaggagc
     9181 agaagcgcct caacgccgcg gccaaccaga agaaggccga cgaggacgcc aaaaagacgc
     9241 cgcccgcggc cagttgaagc cgcgcgccgt cgccctcccc cgcggccccg cgccgccgcc
     9301 gacgatcaca ttcctcaggt gatgctcgac ttccgagtcg ggcatcacct attcttatac
     9361 agagaggtca ga
//
LOCUS       DASVES010000507        27173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_808850, whole genome shotgun sequence.
ACCESSION   DASVES010000507 DASVES010000000
VERSION     DASVES010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 27173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27173
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808850"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3427)
                     /locus_tag="VF240_21425"
     CDS             complement(<1..3427)
                     /locus_tag="VF240_21425"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7177835.1"
                     /translation="MISRKFFALFSTVFLLALCSVAALAQSQTTGRIGGAVVDANGAA
                     VAGAEVSVSSKATGEVRQAVTDESGAYIVSLLPPGDYRVVISIANFKRAVFDTVRVAI
                     TETTTVDAALEVGALTDESVTVTAVGPAIQSESPGLGRVVDTRSVSELPLATRNFTQI
                     LSLSPGTATYLPDNTSVGRNSQNISVNGARVTNNNFQINGIDANSMGTNSAPSLSVPA
                     PETIQEFKVQTSLYDATFGRSGGGNVQAVTKSGSNEFHGGLYAYIRNDALNANNPFLK
                     AAGVERPVLKRNIFGGLIGGPIQKDKMFFFASYQGTRERNGASPINSLSSNILISPGL
                     TDDRSAARLIALYNSRNAVPIAAINPASLALLNAQLPDGTFLIPTPQTASGRYSGSSP
                     SVFDEDQFNANIDYRLSETDTLSGKFFYSNAPQTLVLPSFLGSGPNVPGYGNFQQNNN
                     RLLSLQYVKVFSPTIVNEARIGYNFIRVDATPEEPVLDSQVGISRANAAVFPGLPLIN
                     IGGAGALTIGTSPTIDVQAVAPSTTLADTLSITMGRHQIRAGAEFRYNENNYTLNFFT
                     RGQINFLSFDQFLTGNAFVSVFGSGIGDRSLRAHDYNFFVQDDWKVTNKLTLNLGLRY
                     ELDLPAYDTRGRIATFDPALYNPTQLVNAGSGFVQAGNVIPEYDLASVPNVDKRVVNS
                     IDPNNFAPRVGFAYSPLDSGRLVVRGGYGIFYSRTSFQYITLNVIAPPTYVFGTCVIA
                     CPGQASIPLANPFFPAPSQSAFPTFVPTVALSGTFFDRNLRTPYIQQYNASVQYELAK
                     DYVAEAAYVGTHGVNLFRQIGINQARLASPTSPVVNQVTGAVITTNTPANAALRAPFQ
                     GAGISNFFQNQATAQSNYNSMQLSLTKRFSRGLQFLASYTFAKSIDNASGQGGGPGTG
                     GVLNPGAVGETSPILGDQRDNRANRGVSDFDRPHRFVFSSVYDLPDPIGPEGSAFARV
                     LLNDWQLGGIFTSMSGLPIDIVDSGGASLYGLNGAALSRPNIVGDPFSNVPAGLYFNP
                     AAFARATVLAGQIIPSSGGTATASAAGADFGNVGRNILRGPRQINLDLSIIKRFSIDD
                     SKNVEFRTEFFNLTNHVNFANPISDLNAGAASFGRIISASTNPRLIQFALKFNF"
     gene            complement(3891..5255)
                     /locus_tag="VF240_21430"
     CDS             complement(3891..5255)
                     /locus_tag="VF240_21430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7177836.1"
                     /translation="MANSKEKILIIDDQPSVRFGLRSLLEGEGYRVAEAEDGARGLAV
                     MTENAPQLVLLDLRLPDTDGLELLPRIKAIDDDVPVIILTAHGTIETAIRALKQGAEN
                     FLTKPFDSDGLLILVAKTLEQSRVRRERLLIGLAQERVAAEYFIGESAASLRAHTTIE
                     RLAPSDMTVLLQGETGTGKGMMAQLIHRLSPRAEKPFVTINCAGLSRELLESELFGHE
                     KGAFTGAVASKPGLFELAHEGTMFFDEVAEMEPSIQAKVLHVLEQKKFRRVGGVQEKE
                     VDVRLVAATNKNLQGEIKRGRFREDLFYRLSVMPLTLPPLRERRDDILPLVEHFIRRF
                     NQKLNRRVQGVTPKAEAVLQSYEWPGNIREVRNVVERAVILCSGDLVHASDLPLGREE
                     SWTDGAREAEAHYLSLEELESLHVRRVLAAAGGNLSRAADILGVTRTTLYNKIRKYGL
                     DPSV"
     gene            complement(5255..7648)
                     /locus_tag="VF240_21435"
     CDS             complement(5255..7648)
                     /locus_tag="VF240_21435"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7177837.1"
                     /translation="MRDRFILDPKENILFIDFAGLRIESRAQVEEIARLVEESVAAQG
                     RRVYAVVNYEGTEIAPEIVEYYGERIKELQARHAISTVRYSSSGLTRSVLRYLGAAVD
                     LESNAFTTREEAIRAIRESERRVPTRKPLPLWGMLNPRRSLLGKLSLGWLAGLLVMAV
                     VYAAGAAALSDETARSRAFNVAAAVLALLAGGGATGAILYVSVIRPLRRMESLARSLV
                     LGGAFEPIEPTGEDETGQLARVLNETAGQLRRDIERLSGLYHISLMMGTGTEVRRICE
                     LLTRKTARLLGAEMCVIMLRDEPKRRISAQPPAYGVSDEHARLLTSGLDEENIATKVF
                     HTGEPYMNNEVASGPLTVNGAKAPVAPFLFQDFPGTGGRPGRPSAAEALGVRAVLAVP
                     LRAGERPLGTLMVMNKGGGFVEEDKQLVTIFASQAAQLIVNAQLFERVRHSEERHRQI
                     FESALDGLYRSTRDGRLVTVNPALASMLDYGAAHELEGASLLDLFADRGEADRLLAGL
                     NERGQALDVECELRRRAGDPLPTRISVRAVTDESDGRVYHLGIVRDVTEQKRLSEQLV
                     VSEQLAVVGEMVAGVAHEVRNPLCGITTTVSALARRLEDREEVRPFIEVIMTEAGHLN
                     HLMEQLLEHSRPVRLDGDGADVSQLIREVVGEWTGQAEAKGVALAFECGVELPGLRLD
                     KRKMHGVFVNLIDNALHHTAPGGSVQIVATSPGAGRGSSARHEVRVEVRDTGAGIAPD
                     KLPKIFDPFFTTRTTGTGLGLAIVQKTIHDHGGTITARSEPGKGTAFLIALPLKFGR"
     gene            complement(7748..8152)
                     /locus_tag="VF240_21440"
     CDS             complement(7748..8152)
                     /locus_tag="VF240_21440"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7177838.1"
                     /translation="MNFKPGDFATFTKTITDEDIRAFAALGGDDNSVHVNDEYARKTR
                     FGRRIAHGMLSGSLISTVIGTRLPGEGSIYLSQSFKFVAPVFPGDTVTAKATVKAVRD
                     DKPIVTLETVCTNQHGETVVTGEAVVLVENVE"
     gene            complement(8304..10094)
                     /locus_tag="VF240_21445"
     CDS             complement(8304..10094)
                     /locus_tag="VF240_21445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7177839.1"
                     /translation="MKYPNIYSLFAEQVERYGERNAFYVRRGDEWAGITWEEFGREAH
                     EFACALAARGLRTGGSVCILMGNVPAWPVADLGTIIAGGVSVGLYPTSSAEQCQYIID
                     HADAELVLVDTALQLDKVMRVRAALPKLKTIVALDEAAPRGHEGVVGYRDFLQSGRVR
                     REQLEHGITRRAREARTDDVVIMVYTSGTTGPPKGACLSHRYVLGSVESLQGCVPLDD
                     ADVAFSYLPYCHVAERISGLYTRTYAGATTYFVDDPAKLWDYVLEVKPTVFASLPRFF
                     EKVHARIVSDLDRVAPEERRRFEEALGRGREISRRRQARETVPAELREKYARACESMT
                     DKVKSYFGGRVRLMTSGGAPLLSEIAEFFDAAGLPILQAYGLTENLCVAFNRPDNYRF
                     GTVGPPMPGCEMKTAADGEILVRSAMRFSGYYKEPEKTAAVFDGEGWLLTGDLGEIDR
                     DGFLKITGRKKELIVTSTGKNIAPALIENMLKEHHLISHALVYGDGKSYLVALLTLNA
                     AETLGHARARGIVYTDFADLTRKEETRALVRGIVEGVNERVSQTEAVRKFAILDHDLS
                     VEADEVTPTMKIKRKVVGERYRDVLEGLYK"
     gene            complement(10176..11168)
                     /locus_tag="VF240_21450"
     CDS             complement(10176..11168)
                     /locus_tag="VF240_21450"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEX7177840.1"
                     /translation="MTYAKITGTGSYVPDKVLTNDDLSRMLGEDINEFVSQVVGIRER
                     HVCAEDESTADLATEAARRALDAAGVGPEELDLIVLATDTPEYMSPATSVVVQHRLGA
                     KNAGTFDVNCACAGFVTALDAASKYVVADPATYRNVLVVGAYAMSKFIDWTEKKTATI
                     FADGAGAVVLSASEEGPGFLASKLVADGSYHGHMGIYAGGAHMPISEAVLKEGRFNKV
                     RFVRKYPPEVNIEGWPSIVRDVLAKAGLALEDVTLFLFTQVNLSTIMEVMKRLELPLE
                     RTHTVMGKWGYTGSACIPMVLDDAVRAGKLKRGEVVVMCASGGGLNMACAAFRW"
     gene            complement(11210..12022)
                     /locus_tag="VF240_21455"
     CDS             complement(11210..12022)
                     /locus_tag="VF240_21455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7177841.1"
                     /translation="MPLIQIGDVELYYEAHGEGEPLILIPGFVSGLWLWFKQSEVLAR
                     RFRTIVFDPRGVGRSEGGRTPETIRGCADDVAALLSALGVERAHVLGVSFGGFVAQEF
                     ALTHPRNLGKLVLCCTSYGGARHLPPSASTLQAMASPHGLNTEERTRKNLRPAFAPEF
                     LAGRPEEVEEVTALRLRHPVGELTHLAQVQAAATFDAEARVSRIEAPTLVVTGDEDAI
                     VPPENSRHLAASIPGARLTIIEGAGHMVFIERAEEFNRAVVGFLDERPATRP"
     gene            complement(12268..13983)
                     /locus_tag="VF240_21460"
     CDS             complement(12268..13983)
                     /locus_tag="VF240_21460"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7177842.1"
                     /translation="MKAKAEVRSDSFGQFGLSESLLKAIADVGYEAPSPIQQKTIPLL
                     LEGRDVVGQAQTGTGKTAAFALPILERLEPVSAGVQALVLTPTRELAIQVAEAFHTYA
                     KHMGGVRVLPVYGGQPIHQQLRRLQSGAQVVVGTPGRIMDHLRRETLNLAGLKTVVLD
                     EADEMLRMGFIEDVEWILEHTPAERQTALFSATMPREVRRIADRHLREPAGVEIEHKT
                     LTVPTVEQRYINVSEGHKPDALTRLLEAEGSPGEAALIFARTKVGAAELTEKLQARGY
                     AAESMHGDMSQAQREAVIRRVRGGQVEIVVATDVAARGLDVERISLVINYDMPNDVES
                     YVHRIGRTARAGRAGRAVLFVTPRQQRMKREVEQYTGQRIEPMRVPSEADVAARRVAL
                     FKGRVLKGVEGEGLDLYLSLVEELAEESGRDMTEIAAAAVRLAAGDRPLEVTLEPGPE
                     QLGQPGDGMMRLFIDVGRNGKVGPSDIVGAIANEGGVPGKEIGAIDVHDRFTLVDLPP
                     EYVDQVLQRMKGSRIRNFNANIRVAAQRETERDGAPRARSAKGTGRPKAKAVERKGRT
                     KSASR"
     gene            complement(14302..14964)
                     /locus_tag="VF240_21465"
     CDS             complement(14302..14964)
                     /locus_tag="VF240_21465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7177843.1"
                     /translation="MSVRKVRVLIAEDHVTMREGLKLILENEPDLEVVGEAADGREAL
                     RLARQLLPDVALMDISMPGWGGLQATRGLQETCPQVRVLALTRHADYGYLTEMLRAGA
                     KGYVLKQSPSAELIFAVRAVADGQTYLDPSVTAKVMGGYRRGRAKAAGEEIGESVSER
                     EEEVLRLIALGYSNKEIAARLCVSVKTVEAHKANGMHKLGLGSRTDIVSYAIFRGWMR
                     NT"
     gene            complement(14961..16892)
                     /locus_tag="VF240_21470"
     CDS             complement(14961..16892)
                     /locus_tag="VF240_21470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7177844.1"
                     /translation="MSEQSAELFRLVVENVEDFAVYTKDLEGRVLSWNSGVERLLGYA
                     EGEWVGRHVSVIYTRQDLAQGALDRELKTALAEGRAEDRRWHVRKDGSRFWANGLLML
                     LRDDAGEPLAFAKILRDDTSRRRAEERLKFQANVVSQVSDAVIAIDLEERITFWNSGA
                     ERLYGLEAGEVLGRKLTDAYQYRWLSPADEEAAADSLAKTGSWFGENVHIIKDGRWIQ
                     VESLVSRLEEDDDDAVGLLAVIRDVTERKKAEQALRRSEERLRLTMESAIDYAIFTTD
                     ASGWIETWNVGAERLFGYPEEEAVGLHAEVIFTPEDREQGVPAAEMRQARQEGRSLCE
                     RWHVSKRGRRFYVSGVLVPLRDGHVLTGYAKIVRDLTERRKAEEELRRAHEELEARVE
                     ERTRELQETAGNLLIEAKERAQAESRVRALLRKLVTVQEDERRRIARELHDTLGQQLS
                     ALRLSIDIVKSRAGGRAGVTEETQRMQSIFDRLDADVDFLAWELRPVALDHLGLDAAL
                     QTFVREWSEHFRVEAEYRGFGSDAPRLPPEVETNFYRILQEALQNVHKHAGADHVGVL
                     LERRDAQAVLIVEDNGKGYDPEEEVGADVTRGMGVTNMRERAALVGGTLEVESSPGAG
                     TTIFVRVPVAGPDAGGVDS"
     gene            17366..17749
                     /locus_tag="VF240_21475"
     CDS             17366..17749
                     /locus_tag="VF240_21475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7177845.1"
                     /translation="MSNILIVDDSPTMRRMVTASLRALSGVSFGEASSGLEAIERLAI
                     APVDLMILDLNMPDMHGLEVLDFLRSHQAYQHIPVIVLTTRGDEASRADALAAGAALY
                     LTKPFAPQALAAQAGALLKSHPERA"
     gene            17749..19854
                     /locus_tag="VF240_21480"
     CDS             17749..19854
                     /locus_tag="VF240_21480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX7177846.1"
                     /translation="MSDSHPENGGEFFAQFLDDYFAECDEHLTLVRRHLLALEEQSGR
                     TGVNPSLLDELFRSFHTLKGISAMVELGEAEQLAHHMESYLRALRRQEARLGEEGVEA
                     LMSATTVLEQVIAARRREETAPAIGATVARLEALVSEPESEHGRPSSTRDGDESAADA
                     KSEQSAPPPSGAAGRLENTRTWRFEFTPTPALSERGVNVNSIRARLQEVGEILRAAPH
                     VREQGRIIFEFVVATVVEESAFAGWHEDNLTYAPVGEEAPSTMHTPSPPTGSAPASDT
                     REVEDSRPTPAAPAASRRAPSNVVRVDLARLDELMRLIGELVTSRARLDDGLKQLQRH
                     VAPAEWRGLQETSHAMARQLRDLREGVMRVRMVQVGEIFERMRFVVRDLARESGKEIK
                     LELLGQETEVDKFLVERMMNPLLHLVRNAVSHGLETVEERAALGKPREGKITLRAAAS
                     GDNVLIEIADDGRGIDAERVAARARERGLIAGDVGIDESLLLELICAPGFSTRDEADR
                     ASGRGVGMDVAAKTIEELGGALTLATEKNRGTRFRVELPLTLAIADALIASVAGQRFA
                     FPQTVVREVIEVEASAVKVLENNEIVSYRGGVLPLVRLARLFGLDETTPPRAFHVFVV
                     GSGAGAMGLAVDRAVGQREIVVRGMNDPLVKVAGVAGATDLGDGRVVLILDVAALKYS
                     APAVKHSAGNAKARLPRKR"
     gene            19859..20359
                     /locus_tag="VF240_21485"
     CDS             19859..20359
                     /locus_tag="VF240_21485"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7177847.1"
                     /translation="MSHTQKDAEPFVLFELAGTTYGVRSRFVQQMEMIEHITPVPNAP
                     TAVEGVVLARGQVIPALNLRVRFGFERIPFDLRSRLMVVNQGERTLGLIVDTAREFLR
                     IPESAIEPPPEAITGLSGKYLEGIATIKERMILVLNLAEVIDIGEQQPASAEGLIGDG
                     HVNTDW"
     gene            20340..22544
                     /locus_tag="VF240_21490"
     CDS             20340..22544
                     /locus_tag="VF240_21490"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7177848.1"
                     /translation="MSTQTGNAAVGGLGVNAVRAREHMAQVADAAHEIARIAREVTEG
                     ADAQIHSLDSAVSLANETTASLGETTTQAESIATSTEELVSSVNETAASIEQVSANSS
                     SLAASINETAAAIEESARSIESVTANAQEMATSAAEVTGAMNEIAASVKSVSDDTEAL
                     SVSVNQTAVSMEQMSRSIDSLAGNADDLSVSAEQTASSINEMAASIEEVSAMSENLAS
                     TVEEVSTSMEEMARSVQGVAQNTEKITEAATGAATSAAEMERSLRSVAAMTKRAEEVA
                     GRVSREAEQGGGAAQKSIQGIGRVRASMEQSAAVIREMGKRSNEIGGIVNTINVIAER
                     TNMLSLNASIEAARAGEAGRGFAVVAEEIRNLADRSAQATADIAAIIKALQDVVQEAV
                     AASSEGVRVAEESGRLAEEGSEGLQRILSGVGETTQIVGQIARATEEQLGAGAHVLAS
                     IETTAAQARQAAVATSEQDKAAQEIVKAAAQMRKTAKEVSQAMSEQGRAAREIIKAAQ
                     STTAVATQVRKASTEQAAAAKQVAQAVESMRRGAASTSRALEEQATAGEQVSKESERL
                     ARMIGGVSRAMGEQAAAATQITTAAQSMRQQADQVAKALGEQSRAARDMTAASTSISK
                     EIGLITRSNRQHLTSSEQVLGVLTDIRQITERNAQGVKATLSLTVGLIERAQQLTAAV
                     GDADAGQTGERVAPSASAAPRPNGRPRRSKKGLTPGEAAGNGGPEQSDGSGQ"
     gene            22600..24903
                     /locus_tag="VF240_21495"
     CDS             22600..24903
                     /locus_tag="VF240_21495"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7177849.1"
                     /translation="MGSLTPSDNAIGVFTTDASLVIQIWDAALARLTGIPADAARGKH
                     LAELIPNLEEGRLLARFRRVLDEGVVEVLAPAFHHYLIPCPPSAPSKHFDRMQQRVTI
                     APLREDDRTVGALVTVEDVTARIEREHSLSEQPARADEGVRLSAARALKEEESLEPAR
                     PLLAALGDENWRVRRTAVEGLARRAAPDAIAALLASVRENHQNLGLLNSALQVLAMSD
                     VDTLSQLVEFLNDEDADLRMQAALALGEQRDGRAAPALLGALEDADINVRYHAIEALG
                     KLRAGGAADALAGIAETRDFFLAFPALDALTRIGQAHVAPRVVPLLEDELLREAAADV
                     LGRLGDEETVAPLAALLNTPHAPAPTVARSLAALHDRYEKLYGEGAHIADLTRAAISP
                     TGAQNLLDALNESDSGEPRPLALVVGWLEGAAVERALTRLLGRAGARGEVVEALVRHG
                     SRVTDLLIEQLGSEDLEIRKSAVVALGRIGDARATPALLEALEEDPELVIPAADALAK
                     IGDPRAFDGLLALVGDPHAAVRQAAVGALNSLGSPEMPGRVLPLLEDADPNARESAVK
                     IAGYFGYPECADLLLERCRDEDERVRRAAVEHLPFLEDERATPALVRALRHETPKVRA
                     AAAGAMALAEGPEVSASLIEALGDEDSWVRYFAARSLGRLKIAGSADALAGLARDDKA
                     NHVRIAAVEALGGLGGETAVRAVAPLLQSDEPDIVRVAIAALGRMENPAARPPLLEAL
                     QSPDAEVRAAAAEALGVTKRNRQGDSN"
     gene            24997..25803
                     /locus_tag="VF240_21500"
     CDS             24997..25803
                     /locus_tag="VF240_21500"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX7177850.1"
                     /translation="MRDLIHERTGLFFDNGKGDLLTDKLSPLVVERGFTSLLDYYYLL
                     KYDEASQDEWLNVMNALSVPETYFWREMDQVRALVEWVVPRWFAAPGAGPLKIWSAAC
                     ATGEEPLTIAMALEEAGWLARAPIEIHASDASTAAVEKARRGVYRERSFRNLPAGLRA
                     KYFTQMGATQTWRVDARLHDTVKWGVVNLMSEAESAPWAAANVIFCRNVFIYFSDQTI
                     SRTVRSFAAHIRPPGYLCVAASESLLRLSADFELQEIGNAFVYVKRPGQG"
     gene            25832..26881
                     /locus_tag="VF240_21505"
     CDS             25832..26881
                     /locus_tag="VF240_21505"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX7177851.1"
                     /translation="MDRVLKVLVVDDSAYVRKVVKQMLSRSPFLEVVGTARDGREALE
                     LVVQLDPDVVTCDLIMPELDGVGFVREQMARRPVPIIIMSIASETGEAALTALDEGAV
                     DFVQKPTALATEKIFEVSDELIEKVKAAASIPMGRVKKAPSEPAPAPSRALASVTRKK
                     LVDVVVVGISTGGPQALKQLIPQLPADFPVPMAMVMHMPVGYTEMYARKLDEHSPLEV
                     REAQEGDELRAGLALLAPAGRHLTFRRQAGGAVVAHLDSKPFEMPHRPSVDVLFQSAA
                     EVYGQRVLGVVMTGMGSDGKQGAAWIKAQGGLVYTESEETCVVYGMPSSVVEAGLSDR
                     SVPLDGMAQAIREAV"
     gene            26893..>27173
                     /locus_tag="VF240_21510"
     CDS             26893..>27173
                     /locus_tag="VF240_21510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7177852.1"
                     /translation="MVVDDSALSRRTLRRILETAGYGVVEAEEGMAALELYFIEKPDL
                     VLLDLVMKGMYGLDVLEKLRQMDPLVRVVVLSADIQSTSREMVAAAGAAG"
BASE COUNT         4582 a         8827 c         9076 g         4688 t
ORIGIN      
        1 agaagttgaa cttcagagcg aactggatca gccgcgggtt ggtgctggcc gagatgatcc
       61 gcccgaacga cgctgctccg gcgttgagat cgctgatcgg gttggcgaag ttgacgtggt
      121 tcgtcaggtt gaagaactcc gtgcggaact cgacgttctt tgaatcgtca atcgagaagc
      181 gcttgatgat cgagaggtcg aggttgatct ggcgcggccc gcgcaggatg ttgcggccca
      241 cgtttccgaa gtcggcgccg gccgcggacg ccgtggccgt gccgccggaa ctcggaatta
      301 tttgtccggc taagacggtc gcgcgggcga acgccgccgg gttgaagtag agaccagccg
      361 ggacgttgct gaacggatcg ccgacgatgt tgggccgcga gagcgccgcg ccgttcaggc
      421 cgtagagcga cgcgccgccc gagtccacga tgtcaatcgg cagccccgac atcgacgtga
      481 agatgccgcc gagctgccag tcgttgagca ggacgcgcgc gaaggccgag ccttcggggc
      541 cgatggggtc gggcaggtcg tagacggagc tgaagacgaa acgatgcggg cggtcgaagt
      601 ctgacacccc gcggttcgcg cggttgtcgc gctgatcgcc gagtatcggg gaggtctcgc
      661 cgacggcgcc gggattgagg acgccgcccg tgccggggcc gccgccctga cccgaggcgt
      721 tatcaatcga cttcgcgaaa gtgtaggagg cgaggaattg cagcccgcgc gagaagcgtt
      781 tggtcaaact caactgcatc gagttgtaat tcgactgagc ggtcgcctga ttctggaaga
      841 agttggaaat ccccgctccc tgaaacggcg cgcgcagggc ggcgttcgcg ggcgtattgg
      901 tcgtgatcac cgcgccggtc acctgattga cgactgggct ggtcgggctg gcgaggcgcg
      961 cctgattgat tccaatctgg cggaacaggt tgacgccgtg cgtgccgacg taagccgcct
     1021 cggccacgta atctttggca agctcgtact gcacgctcgc gttgtactgt tggatgtacg
     1081 gcgtgcggag gttgcggtcg aagaacgtgc cggagagcgc gaccgtcggc acgaaagtcg
     1141 ggaaggcgct ctgcgacggc gcggggaaga acggattggc gagcggtatc gaagcctgtc
     1201 cggggcaggc gatgacgcac gtgccgaaga cgtaggtcgg cggcgcgatg acgttcaagg
     1261 tgatgtactg gaacgaggtg cgcgagtaga agatgccgta gccgccgcgc acgacgaggc
     1321 ggccgctgtc gagcggcgag taagcgaagc cgacgcgcgg cgcgaagttg ttcgggtcga
     1381 tgctgttgac cacgcgcttg tccacgttcg gcacgctcgc cagatcgtac tcgggaatca
     1441 cgttgccggc ctgcacgaaa ccgctgccgg cgttgacgag ctgcgtcggg ttgtagagcg
     1501 ccgggtcgaa ggtggcgatg cgcccgcgcg tgtcgtaggc cggcaggtca agctcgtagc
     1561 gcaggccgag gtttaaggtc agcttgttgg tgactttcca atcgtcctgc acgaagaagt
     1621 tgtaatcgtg cgcgcgcagg gagcggtcgc cgatgccgct gccgaagacg gagacgaagg
     1681 cgttgcccgt gaggaactga tcgaagctga gaaagttgat ctggccgcgc gtgaagaagt
     1741 tcagagtgta gttgttttcg ttgtagcgaa actcggcgcc cgcgcgaatc tggtggcgac
     1801 ccattgtgat cgagagcgtg tcggccagag tcgtcgaggg cgcgacggcc tgcacgtcga
     1861 tggtcggcga agtgccgatg gtgagagcgc ccgctccgcc gatgttgatg agcggtagtc
     1921 cgggaaacac ggccgcgttc gcgcgcgaga tgccgacctg cgaatcgagg acgggttctt
     1981 ccggcgtggc atcgacgcgg atgaagttgt agccgatgcg cgcttcgtta acgatggtcg
     2041 ggctgaagac cttgacgtat tgcagcgaga gcaggcggtt gttgttctgc tggaagttgc
     2101 cgtagccggg gacgttgggg ccgctcccca ggaagctcgg cagcacgagc gtctgcggcg
     2161 cgttcgagta gaagaacttg ccggagagcg tgtccgtctc gctgagacgg taatcgatgt
     2221 tggcgttgaa ctggtcttcg tcgaacaccg aggggcttga gccggagtaa cgcccgctgg
     2281 cggtctgcgg cgtcgggatc aggaaagtgc cgtcgggaag ttgcgcgttg agcagggcga
     2341 gcgaggccgg gttgatcgcg gcaatcggga cggcgtttcg cgaattatag agcgcgatga
     2401 gacgcgccgc cgagcggtca tccgtcaggc cgggcgagat cagaatgttc gacgagagac
     2461 tgttgatggg acttgcgccg ttgcgctcgc gcgtgccctg ataggaggcg aaaaagaaca
     2521 tcttgtcctt ctgtatgggg ccgccgatca gcccgccgaa gatgttccgc ttgaggacgg
     2581 ggcgctcgac gcccgccgcc ttcaggaacg ggttgttggc gttcaaggcg tcgttgcgga
     2641 tgtacgcgta gaggccgccg tggaactcgt tcgagccgct cttcgtgacg gcttgcacgt
     2701 tgccgccgcc cgagcgcccg aaggtggcgt cgtagagcga ggtctgcacc ttaaactcct
     2761 gaatcgtctc gggcgcgggc accgagagcg agggggcgga gttggtgccc atcgagttgg
     2821 cgtcgatgcc gttgatctgg aagttgttgt tggtgacgcg cgcgccgttg acggagatgt
     2881 tctgcgagtt gcgcccgacc gaagtgttgt ccggcaggta ggtggccgtg cccggcgaga
     2941 ggctgagtat ctgcgtgaag ttgcgcgtcg ccagcggcag ctccgacacg gagcgcgtgt
     3001 caaccacgcg tccgagaccg gggctttcgg actggatggc cggcccgacc gccgtcaccg
     3061 tcaccgactc atcggtcagc gcgccgacct ccagcgccgc gtcaacggtc gtcgtctccg
     3121 tgatcgccac gcggacggtg tcgaagacgg cccttttgaa gttcgcgatg gagatgacga
     3181 cgcgatagtc tccgggaggg agcagcgaca cgatgtaggc gcccgactcg tcggtgacgg
     3241 cctgtcggac ttcgccggtc gccttgctgg agacggagac ctcagcgccg gcgaccgccg
     3301 cgccgttggc gtctacgacg gccccgccga tgcggcccgt cgtctggctc tgggcgagcg
     3361 cggcgacgct acagagggcc agcaggaaca cggtcgagaa caacgcaaaa aatttccttg
     3421 agatcatcaa atcctctcct tcgcttaaac tatggggcca gtcgaatcgg ggagccgcgc
     3481 tcggcgcgaa ttaaagacac tatcctcagt tgagtgaaac tactccgggc gtgacttgcc
     3541 gtagatgcag tgcaactatc gcgccgcagc cacgctaccc gcgcgaggcc accggagggg
     3601 tgtttcgatt tgtttcccga cacaactatt gaacccgttt ttgtgccggg gccacacgac
     3661 cgccggcacg cggcccagcg aacgggaaag ccgcgtcggg tcgagttgcc tagttattgg
     3721 acagtccgat tgaacagtgc tcaatcttta gacagcgacg gcgggacttc agagggaacg
     3781 tgaattcaac atgagagaca ttgcggactt gatttcctgc cccggagggg caggaaagtg
     3841 agccgccgcc cttcctcacg tcgaactcac atagagagaa gggtcaagtc tcacacggag
     3901 gggtcgaggc cgtatttgcg aatcttgttg tagagcgtgg tgcgggtgac gccgaggatg
     3961 tcggcggcgc ggctgaggtt tccgcccgcg gcggccagaa cccggcggac gtgtagcgac
     4021 tccagctctt cgagggagag gtagtgagcc tcggcctcgc gcgcgccgtc tgtccatgat
     4081 tcttccctgc cgagcggtag gtcgctcgcg tggacgaggt cgccggagca gaggatgacg
     4141 gcgcgctcga cgacgtttcg cacctcgcgg atgttgcccg gccactcgta gctttggagc
     4201 accgcctcgg ccttcggcgt cacgccctgc acgcgccggt tgagtttctg gttgaagcgg
     4261 cggatgaagt gttcgacaag cggcaggatg tcgtcgcgcc gctcgcgcaa tggagggagc
     4321 gtcaacggca tgacgctcag acggtagaag aggtcttcgc ggaagcgccc gcgcttgatc
     4381 tcgccttgca ggttcttgtt cgtggcggcg acgaggcgca cgtcaacctc tttctcctgc
     4441 acgccgccca cccgccggaa cttcttctgc tcaagcacgt gcagcacctt cgcctgaatc
     4501 gaaggctcca tctcggcgac ttcgtcgaag aacatcgtgc cctcatgcgc cagctcgaac
     4561 agccccggct tgctcgccac cgcgcccgtg aaagcgccct tctcgtggcc gaacagctcc
     4621 gactcaagca gctcgcgcga caggcccgcg cagttgatcg tcacaaacgg cttctcggcg
     4681 cgcgggctga gccggtggat gagctgcgcc atcatcccct tacccgtgcc ggtctccccc
     4741 tgaagcagca cggtcatgtc cgatggggcg agccgctcga tggtggtgtg cgcgcgaagg
     4801 ctcgccgcgc tctctccgat gaaatactcg gcggcgacgc gctcctgagc gagaccgatg
     4861 agcaggcgct cgcggcgcac gcggctctgt tcgagcgtct tggcgacgag gatgaggagg
     4921 ccgtcagagt cgaacggctt ggtgagaaaa ttctccgcgc cttgtttaag tgcgcggatg
     4981 gcggtctcga tggtgccgtg tgcggtgagg atgatgacgg gcacgtcgtc gtcgatagcc
     5041 ttgatgcgcg gcagaagctc aaggccgtcc gtgtcgggga gacgcaggtc gagcaggacg
     5101 agctgcggcg cgttctccgt catcacggcg agacccctcg cgccgtcttc ggcctcggcc
     5161 acgcggtagc cctcgccttc gagcaggctc cgcaggccga agcggacgga cggctggtcg
     5221 tcgatgatga gaattttttc cttactgttt gccattatcg cccgaacttc agcggcagcg
     5281 cgatgaggaa ggccgtcccc ttccccggtt cgctgcgagc cgtgatcgtc ccgccgtggt
     5341 cgtgaatcgt cttctgcacg atggcgaggc cgaggcccgt ccccgtcgtg cgcgtcgtga
     5401 agaagggatc gaaaatcttc ggcagcttgt cgggtgcgat acccgcgccc gtgtctctca
     5461 cttccacgcg aacttcgtga cgcgcggaac tcccgcgccc cgcgcccggc gacgtggcga
     5521 cgatctggac gctcccgccc ggcgcggtgt gatgcagcgc gttgtctatc aggttgacga
     5581 agacgccgtg catcttgcgc ttgtcgaggc gcaggcccgg caactccacg ccgcactcga
     5641 aggcgagcgc cacgcctttg gcctcggcct gccccgtcca ttcgccgacc acctcgcgga
     5701 tcaactggct cacgtccgcg ccgtcgccgt ccagacggac ggggcggctg tgctccagca
     5761 attgctccat caagtgattg aggtgtccgg cctccgtcat aatcacttca atgaacgggc
     5821 gcacctcctc gcggtcttcg agccggcgcg cgagcgcgct caccgtggtc gtgatgccgc
     5881 acagaggatt ccgcacctcg tgcgccacgc ccgccaccat ctcgccgacc acggccaact
     5941 gctccgagac gaccaactgc tcggaaagcc gtttctgttc cgtcacgtcg cggacgatgc
     6001 cgaggtgata gacgcgcccg tccgactcgt ccgtgacggc gcggacgctg atacgcgtcg
     6061 ggagcggatc gcccgcgcgg cggcgcagct cgcactcaac gtcgagcgcc tgcccacgct
     6121 cgttcaagcc tgcgagcagg cggtcggcct ccccgcggtc ggcgaacagg tcgagcaggc
     6181 tggccccctc caactcgtgc gccgcgccgt agtcgagcat cgaggcaagc gcggggttga
     6241 cggtgacgag gcggccatct cttgtgctgc ggtagaggcc gtcgagcgcg ctctcgaata
     6301 tctgccggtg acgctcctcc gagtgacgaa cgcgctcgaa cagttgcgcg ttgacgatca
     6361 gttgcgccgc ctgcgaggcg aagatcgtca cgagttgctt atcctcttcg acgaacccgc
     6421 cccccttatt catcaccatg agcgtgccga gcgggcgctc gccggcgcgc aagggcacgg
     6481 ccagcacggc ccgcaccccc aacgcctcgg cggccgaagg ccgaccgggc cgtccacccg
     6541 tccccggaaa atcctggaag agaaaagggg cgacgggggc tttcgcgcca ttaactgtga
     6601 gcgggccgga ggcgacttcg ttgttcatgt acggctcgcc cgtgtgaaag accttcgtgg
     6661 cgatattctc ttcgtcgagg ccggaggtaa gcaggcgcgc gtgctcgtcg ctcacgccgt
     6721 aggcgggggg ttgcgcgctg attcgccgct tcggctcgtc gcggagcatg atgacgcaca
     6781 tctccgcgcc gagaagccgc gcggttttgc gcgtcagcaa ctcgcagatg cgtctcacct
     6841 ccgtccccgt ccccatcatc aaagagatgt gatagagacc gctcaagcgc tcgatgtcgc
     6901 ggcgcagttg cccggcggtc tcgttgagca cgcgcgcgag ctgccccgtc tcgtcctcgc
     6961 ctgtcggttc aatcggctcg aacgccccgc cgaggacgag gctccgcgcc agagactcca
     7021 tccttcgcag gggcctgatg acggagacgt agagaatcgc gcccgttgcc ccgccgcccg
     7081 cgaggagcgc caacaccgcc gcggcgacgt tgaaagcgcg cgaccgcgcc gtctcgtccg
     7141 agagcgccgc cgcccccgcc gcgtagacga ccgccatgac gaggagaccc gcgagccatc
     7201 cgagcgagag cttgccgagc agactgcggc gcgggttgag cattccccac agcgggagcg
     7261 gcttgcgcgt cgggacgcgg cgctccgatt cgcggatggc gcggatggct tcctcgcgcg
     7321 tcgtgaaggc gtttgattcg aggtccacgg ccgcgcccag atagcgcagc accgagcgcg
     7381 tcagcccgct cgaagagtag cgcaccgtcg agatggcgtg gcgcgcttgc agctccttga
     7441 tgcgctcgcc gtaatactca acgatctcgg gcgcgatctc cgtcccctcg tagttgacga
     7501 ccgcgtagac gcgccgcccc tgcgccgcga ccgactcctc gacgagccgc gcgatctcct
     7561 cgacctgcgc gcggctctcg atacgcaagc ccgcgaagtc tatgaagagg atgttctctt
     7621 tgggatcgag gatgaatctg tcgcgcacgc gctcggcctc actccggcca gaaaatatac
     7681 tgcgaccgcc cgccttaaac acaagccgac ggtcgccgcc cggttcgaga tgtaatgata
     7741 ctggaaatca ctccacgttt tcgaccagca ccaccgcctc gcccgtgacg accgtctcgc
     7801 cgtgctggtt ggtgcagacc gtctccagcg tcacgatggg cttgtcgtcc ctcacggcct
     7861 tgaccgtggc cttcgccgtc accgtgtcgc cgggaaagac cggcgcgacg aatttaaaac
     7921 tctggctcag gtaaatcgat ccttcgccgg gcagccgcgt cccgataacc gtcgagataa
     7981 gcgacccact caacatcccg tgcgcgatgc gccgcccaaa gcgcgtctta cgcgcatact
     8041 catcgttcac gtggacgctg ttatcgtcgc ccccgagggc ggcgaaggcg cggatgtctt
     8101 cgtcggtaat cgtcttcgta aaggttgcga aatctccggg tttaaagttc aactttcacg
     8161 ctcctttgat ggtgtgcggg tttgtaccgc aggcggggtc gaggcggaag cccgaccgtg
     8221 agggagggcg tgttagttac gtcagcgccg gccccggcgc cgcccgcctt aaaacgccct
     8281 ccctcacggt cgggcttccg cctttatttg tacaacccct ctaacacgtc tctgtaacgc
     8341 tcgccgacga ccttgcgctt gatcttcatg gtcggcgtca cttcgtccgc ctcgacgctc
     8401 aggtcgtgat cgaggatggc gaacttcctc accgcctccg tctgcgagac tctttcgttg
     8461 acgccttcga cgatgccgcg gacgagcgcg cgcgtctctt ccttccgcgt caggtcagcg
     8521 aagtctgtgt aaacaatccc gcgcgcgcgc gcatgcccca gcgtctccgc cgcgttgagc
     8581 gtgaggagcg caaccagata gctcttgccg tcgccgtaga cgagcgcgtg gctgatgagg
     8641 tgatgctcct tgagcatgtt ctcgatgagg gcgggggcga tgttctttcc cgtcgaggtg
     8701 acgatcaact ctttcttgcg gcccgtgatc ttgaggaatc cgtcacggtc aatctcgccg
     8761 aggtcgcccg tgagcagcca gccttcgccg tcgaaaactg cggccgtctt ctccggctcc
     8821 ttgtaatagc cgctgaacct catcgcgctg cggacgagga tttcgccgtc ggcggcggtc
     8881 ttcatttcgc agccgggcat cggcggcccc acggtgccga acctgtaatt gtccggccgg
     8941 ttgaaggcga cgcagaggtt ttccgtcagt ccgtaagctt gcaggatggg caggcccgcc
     9001 gcgtcgaaaa actccgcgat ctctgatagc agcggcgcgc cgccggaagt catcagccgg
     9061 acacgaccgc cgaagtaact cttaaccttg tccgtcatag actcgcacgc gcgcgcgtac
     9121 ttctctctca actcggctgg cacggtctcc ctcgcttgtc ggcggcgact tatttcgcgg
     9181 cccctgccga gcgcctcttc aaagcgccgc cgctcttcgg gcgccacacg gtcgaggtct
     9241 gacacgatgc gcgcatgcac cttctcgaag aaacgcggca gggaggcaaa caccgtcggc
     9301 ttcacttcga gcacgtagtc ccagagcttc gccgggtcgt caacgaagta tgtggtcgcg
     9361 cccgcgtagg tgcgcgtgta gaggccggag atacgttccg cgacgtggca gtagggaaga
     9421 taggagaagg ccacgtccgc gtcgtcgagc gggacacatc cctgcaacga ctcgacgctg
     9481 cccagcacgt aacgatgact gaggcacgcc cccttcggcg gccccgtcgt ccccgacgtg
     9541 taaaccataa tcaccacgtc atcagtccgc gcctctcgcg cacgccgcgt gatgccgtgc
     9601 tccaactgct cgcggcgcac gcgccccgac tgcaaaaagt cccggtagcc gaccacgccc
     9661 tcgtgcccgc gcggggccgc ttcgtcgagc gccacgattg tcttcaactt cggcagcgcc
     9721 gcgcgcacgc gcatcacctt gtcgagctgg agggcggtgt ccaccagcac caattcggcg
     9781 tccgcgtggt cgatgatgta ctgacactgc tcggcggaac tggtggggta gaggccgacg
     9841 ctcacgcccc ccgcgatgat cgtgccgagg tcggcgaccg gccacgcggg gacgttgccc
     9901 atgaggatgc agaccgagcc gcccgtcctt agtccccgcg cggccagcgc gcaggcgaac
     9961 tcgtgggcct cgcgcccgaa ctcctcccac gtgatgcccg cccattcgtc gccccgccgc
    10021 acgtaaaaag cgttgcgctc gccgtatcgt tcgacctgct cggcgaagag tgagtagatg
    10081 ttcgggtact tcataagaaa gtgagcagtg agcggtgagc agtgagcagt ttaagacacg
    10141 gtaagctttg actgctcact gctcactgct tttcgctacc agcgaaaagc cgcgcacgcc
    10201 atgttgaggc cgccgcccga agcgcacatg acgaccacct cgccgcgctt gagcttgccc
    10261 gcgcggacgg cgtcatccaa gaccatcggg atacatgccg agccggtgta gccccatttc
    10321 cccatgacgg tgtgcgtgcg ctccaagggg agttcgagcc gcttcatgac ctccatgatg
    10381 gtgctcaggt tgacctgcgt gaagaggaaa agcgtcacgt cttccagcgc caggcccgct
    10441 ttcgccagca cgtcccggac gattgagggc cagccttcga tgttgacctc cggcggatac
    10501 ttccgcacga agcggacttt gttgaacctg ccttccttta gcaccgcctc gctgatcggc
    10561 atgtgcgcgc cgcccgcgta gatgcccatg tgtccgtgat agctcccgtc ggcgacgagc
    10621 ttcgacgcga gaaagcccgg cccttcttcc gacgcactga gcacgaccgc gcccgcgccg
    10681 tcggcgaaga tggtcgccgt cttcttctcc gtccagtcga tgaacttgct catcgcgtat
    10741 gcgccgacga cgagtacgtt ccggtacgtc gccgggtcgg cgacgacgta tttcgacgcc
    10801 gcgtcgagcg cggtgacgaa gccggcgcag gcgcagttca cgtcgaacgt gccggcgttc
    10861 ttcgcgccga gccggtgctg aacgacgacc gacgtggccg gcgacatgta ctccggcgtg
    10921 tccgtcgcga gcacgatgag gtcaagctct tccgggccga ctcccgccgc gtcgagcgcg
    10981 cggcgcgcgg cttcggtcgc caggtcggcg gtgctttcgt cctcggcgca gacgtggcgc
    11041 tcgcgtatgc cgaccacctg cgagacgaat tcgttgatgt cctcgccgag catccggctc
    11101 aggtcgtcgt tcgtgagcac cttatcgggg acgtaggagc ctgtgccagt aattttcgcg
    11161 tatgtcatat cgtcgtctgt cgaagtgaag tcgaagtgaa tcaggtttgt taaggtcgtg
    11221 tcgcgggccg ctcgtcgagg aatccgacga ccgcccggtt gaactcttcg gcgcgctcga
    11281 tgaaaaccat gtggcccgcg ccttcgatga ttgtgagcct cgcgcccggt atgctcgccg
    11341 cgaggtggcg cgagttttcc ggcgggacga tggcgtcctc atcgcccgtg acgacgagcg
    11401 tcggcgcttc gatccgcgag acccgcgcct cggcgtcgaa agtcgcggcg gcctgcacct
    11461 gcgcgagatg tgtcagctcg ccgacgggat gacgcagacg cagcgccgtc acttcttcga
    11521 cttcttcggg tcgccccgca aggaattcag gggcgaacgc cggccgcaga ttcttgcgcg
    11581 tgcgctcctc tgtattcagt ccatgtgggg aagccatcgc ctgcaaagtg ctcgcggacg
    11641 gcggcagatg gcgtgcgccg ccgtagctcg tgcagcacaa cacgagctta ccgagattgc
    11701 gcgggtgcgt gagcgcgaac tcctgcgcga caaagccgcc gaacgagacg cccaggacgt
    11761 gcgcccgctc gacgccgagc gccgagagca gcgccgccac gtcatccgca catcccctaa
    11821 tggtctcagg cgtgcgcccg ccctccgagc gtcccacgcc gcgcgggtcg aagacgatgg
    11881 tgcggaacct tcgcgcgaga acttcgctct gtttgaacca gagccacaat ccagacacga
    11941 agccgggaat caaaatcagc ggctcgccct cgccatgggc ctcgtaataa agctcgacat
    12001 ctccaatctg aatcagcggc atagcgtcgg tatgaaatca atttgcgacg gcacgattcg
    12061 tcgcacccgg cgtcattttc gagaggtcga ctctaaggtg caaggatggg tcgcgtccgc
    12121 gggctccaca tgccgggaca ctgtgggggt aggcagagct tgtcccttct gtatttaaga
    12181 aaagtagcgg acaggctcgc ggcaaggggc tggctacgga ttctcaccat tacccgagcg
    12241 ttggacgcgt tctcgcggaa tttgtgctca acgactggca gacttggtgc ggcccttacg
    12301 ttcgaccgcc ttagccttcg gcctgccagt tcctttcgca gaccgtgcgc gaggggcccc
    12361 gtcccgctcc gtctctctct gggccgccac ccggatgttc gcgttgaagt tgcgaatacg
    12421 gctacctttc atgcgctgga gcacctgatc gacgtactcc ggcgggaggt caacgagcgt
    12481 gaaacggtcg tggacgtcaa tcgcgccgat ctctttaccc ggcacgccgc cttcattggc
    12541 gatggcgccg acgatgtcgg acgggccgac cttgccgttg cggcccacgt cgatgaacag
    12601 gcgcatcatg ccgtcgcccg gctgccccag ctgttctggc cccggctcca gcgtgacctc
    12661 cagcggcctg tctcccgcgg ccaagcggac ggccgccgcg gctatctcgg tcatgtcgcg
    12721 cccgctttcc tcggccaact cttcgacaag tgacaggtag aggtccagcc cctcaccctc
    12781 cacacccttg aggacgcgcc ccttgaatag cgccacgcgc cgcgcggcga cgtcggcttc
    12841 ggagggcacc ctcatgggct cgatgcgctg gcccgtgtac tgctcgacct cgcgcttcat
    12901 ccgctgctga cgcggagtca cgaagagcac cgccctgcct gctcgccccg cccgcgccgt
    12961 ccggccgatg cggtggacgt aggactccac atcgttcggc atgtcgtagt tgatgaccag
    13021 gctgatccgc tcgacgtcga ggccgcgggc cgccacgtcg gtggcgacga cgatctcgac
    13081 ctgcccgccc cgcacccttc ggatgaccgc ctcgcgctgg gcctggctca tgtcgccgtg
    13141 catggactcg gcggcgtagc cccgtgcctg gagcttctcg gtcagctcgg ccgctccgac
    13201 tttcgtccgg gcgaagatga gcgccgcctc gccgggactg ccctcggcct cgagcaggcg
    13261 cgtcagggcg tccggcttat gcccctcgga gacgttgatg taacgctgct cgacggtcgg
    13321 caccgtgagc gtcttgtgct cgatctcgac gcccgccggc tccctcaggt gccggtcggc
    13381 gatgcggcgc acctcgcgcg gcatcgtcgc cgagaagagc gccgtctgtc tctcggccgg
    13441 agtatgttcc aggatccatt ccacgtcctc gatgaatccc atgcgcagca tctcgtcggc
    13501 ctcgtcgagc acgacggttt ttagtcccgc gaggttcagg gtctcacgcc gcaagtggtc
    13561 catgatgcgg ccgggggtgc cgacgacgac ctgcgcgccg ctctggagcc ggcggagctg
    13621 ctggtggatg ggctggccgc cgtagaccgg caggacgcgg acgcctccca tgtgcttggc
    13681 gtaggtgtgg aaggcttcgg ccacctgaat ggcgagctcc cgcgtgggag tgagtacgag
    13741 ggcctgcacg ccggcgctga cgggttcgag cctctccagg atgggcaggg cgaaggcggc
    13801 cgtcttaccc gtcccggtct gggcctggcc gacgacgtcg cgcccttcga ggagcagcgg
    13861 gatcgttttc tgctggatag gtgagggggc ctcatagccg acatcggcta tcgccttcag
    13921 tagcgactcg ctcaggccga actggccgaa agagtccgag cggacttccg ctttcgcctt
    13981 catcggaata cctcttcatg acagggcggc tcaacgccgt ttcaccaggt aggctgtctg
    14041 cctcgcccgc gtgagccgtc aagatgatcg taaatgcccg gaattaatct caggtaattc
    14101 tatccgatat caacttcttt ttgtgtacgg tgaaagacta aagttagaac cgacacaatc
    14161 gtcacccgct tcaaagcggg cggccgttat cgccgagcag gttggagggg cgccggaggt
    14221 cgagagcagg ccgggcgggg gatgccctct acgtgcgcgt ccccgccgac agcggcaggc
    14281 gcttacggcc ctttagcgcg gctaggtatt ccgcatccag ccgcggaaga tggcgtagct
    14341 gacgatgtcc gtcctgctcc cgagtcccag cttgtgcatg ccgttcgctt tgtgcgcctc
    14401 caccgtcttg acgctgacgc agaggcgcgc ggcgatctcc ttgttgctgt agccgagcgc
    14461 gatgaggcgc agcacctcct cctcccgctc gctgactgac tccccgatct cctccccggc
    14521 ggctttcgcc cgtccgcgcc tatagccgcc catgacttta gccgtcacgg aagggtcgag
    14581 gtaggtctgc ccgtcggcga ccgcccgcac cgcaaatatc agctccgccg acgggctctg
    14641 cttgagcacg tagcctttgg ccccggcccg aagcatctcg gtcaggtagc cgtagtcggc
    14701 gtggcgcgtc agggccagca cacgcacctg cgggcaagtc tcttgaagcc cccgcgtcgc
    14761 ctggaggccg ccccaccccg gcatggagat gtccatcaac gccacgtccg gcagcagttg
    14821 gcgcgcgagc cgcagcgcct cccgcccgtc ggcggcctcg ccaacgacct ccaggtcggg
    14881 ctcgttttcg aggatcagct tcagcccctc gcgcatcgtc acgtgatcct cggcgatgag
    14941 gactcgcacc ttcctcacgc tcatgagtcc acgcctcctg cgtcgggtcc ggcgacgggg
    15001 acacgcacga agatggtcgt tcccgctccg ggtgaactct cgacctccag cgtgccgccg
    15061 acgagcgccg cgcgctcccg catgttcgtc acgcccatcc ccctggtgac gtcggcgccg
    15121 acctcctctt cagggtcgta gcccttgccg ttgtcttcca cgatgagcac ggcctgagcg
    15181 tcgcgccgtt cgagcaggac gccgacgtgg tcggccccgg catgcttgtg gacgttctgg
    15241 agcgcctcct ggaggatgcg gtagaagttc gtctccacct ccggcggcag gcggggggcg
    15301 tcagagccga agccgcggta ctcggcctcc acccggaaat gctcgctcca ctcccgaacg
    15361 aaggtctgga gcgcggcgtc gagtccgaga tggtcgaggg cgacgggcct taattcccag
    15421 gcgaggaagt ccacgtccgc gtcgagccgg tcgaagatcg actgcatccg ctgagtctcc
    15481 tccgtcaccc cggcgcgacc ccccgccctg gacttaacga tatcgatgct caggcgcagc
    15541 gcgctgagct gctggccgag cgtgtcgtgc aactcgcggg cgattcttcg cctctcgtcc
    15601 tcctgcacgg tgacgagctt tctgaggagg gcgcggacgc gactttccgc ctgggcgcgc
    15661 tccttagcct cgatcagcag attgccggcc gtctcctgca actcgcgggt gcgctcctcg
    15721 acccgcgcct ccaactcctc gtgcgcgcgg cgcaactctt cttccgcctt ccggcgctcg
    15781 gtgaggtcgc ggacgatctt ggcgtagccc gttaagacgt gaccgtcgcg cagcggcacc
    15841 aggacaccgc tcacgtagaa gcgtcggccg cgcttgctga cgtgccaacg ttcgcacaaa
    15901 gaccggccct cctggcgcgc ctgccgcatc tccgccgcgg ggacgccctg ctcccggtct
    15961 tcaggcgtga agatgacctc ggcgtgcagg ccgaccgcct cttcttcggg gtagccgaac
    16021 aaacgttccg cgccgacgtt ccacgtttcg atccacccgc tggcgtccgt cgtgaagatg
    16081 gcgtagtcga tggcgctctc catcgtcagg cgcagacgct cttcgctgcg cctcaacgcc
    16141 tgctccgcct tcttgcgctc ggtgacgtct ctgatcaccg ccagtaggcc gacggcgtcg
    16201 tcgtcatcct cttccagtct gctgactaat gactcgacct gtatccatct cccgtccttt
    16261 atgatgtgaa cgttctctcc aaaccaggag cccgtcttcg ccagcgagtc ggcggcggcc
    16321 tcctcgtcgg cgggcgaaag ccagcggtat tgataggcgt cggtcagctt gcggcccagc
    16381 acctcgccgg cctccaggcc gtagagccgt tcagcgccgc tgttccagaa ggtgatgcgc
    16441 tcctccaggt cgatggcgat cacggcgtcc gacacctgcg acacgacgtt ggcctgaaac
    16501 ttgaggcgct cttcggcccg cctgcgcgac gtgtcgtcgc gcaaaatttt ggcgaaggcg
    16561 agcggctcgc ccgcgtcgtc ccggagcagc atgagcaggc cgttggccca gaaccgcgag
    16621 ccgtccttgc gcacgtgcca gcgccggtcc tcggcgcgcc cctccgccag cgcggtcttc
    16681 agctcacggt cgagcgcgcc ctgcgcgagg tcttgccggg tgtagatgac ggagacgtgc
    16741 cgcccgaccc actcaccttc cgcgtagccg agcagacgtt cgaccccgga gttccagctc
    16801 aggacacgcc cctccaggtc tttcgtgtag acggcgaagt cctcgacgtt ctccacgacg
    16861 aggcggaaca actccgcact ctgctcagac atctccggct tcaattctga cctcacctga
    16921 ccgccgagcg ccacgctggg cgccgagctt ccgtaaacgc cgcgtagtga taggtgaatg
    16981 aatttatatt actacacgaa tccccccgtt ccgcgtcaat aagttagcgc accatctcgt
    17041 tagagattta aggggaagcg ggaagttagc aagaaggccg gccaccctga atatttagat
    17101 ggtgctcgac gcgatgcgtc cgggaacatt aacgtttccg tcacgaatgc cctcgcctgg
    17161 tctgccgtgt tcgccgtcgt cctgagactc cgtgaccaac gcggtgacgc tcgtcctcgc
    17221 cgccgccatc gtctggatga agctaaagtt taagtaggca agactcttat aacgtatggc
    17281 ctgctgaggg aacgcctgtt atcattaact ttagccacca ataaggaagt taaatccccg
    17341 acagggggat gcgcccggcc gccgtatgag caatatttta attgttgacg actcgccgac
    17401 catgaggcgg atggtcacgg cctcgctgcg cgccctctcg ggcgttagct tcggggaagc
    17461 gagcagcggt ctcgaagcca tcgagcgtct ggcaatcgcc cccgtagact tgatgattct
    17521 cgacttgaac atgccggaca tgcacggcct ggaggtgttg gacttcctgc gcagccatca
    17581 ggcgtatcaa cacatccccg tcatcgtgct gacgaccagg ggggacgagg cgagccgcgc
    17641 ggacgcgctc gccgccggcg ccgccctgta tttaaccaaa ccgttcgccc cacaggcatt
    17701 ggccgcgcag gcgggcgcct tgttgaaatc tcatccggaa agggcctagt gtccgattcg
    17761 caccccgaaa acggcggcga gttttttgct cagttcctcg acgattattt cgccgagtgc
    17821 gatgagcacc tgaccctcgt gcgccgtcac ctgctcgcgc tcgaagagca gtccgggcgg
    17881 acgggcgtca acccgtctct actcgacgag ctgttccgca gcttccacac gctcaagggc
    17941 atctcggcca tggtcgagct gggcgaggcg gaacaactcg ctcatcacat ggagagttac
    18001 ctgcgggctt tgcgccggca ggaggcgcgg ctcggcgagg aaggcgtcga ggcgctcatg
    18061 tcggccacga ccgtgcttga gcaggtcatc gccgcacgcc gcagagagga gacggccccg
    18121 gccatcggtg cgaccgtcgc gcgtcttgag gcgttagtct cagaacccga gtcggaacac
    18181 ggacggcctt cgtccacacg ggacggcgac gaaagcgcgg ccgatgcgaa gtcagaacag
    18241 agtgcgccgc cgccgtccgg cgccgcgggt cgcctggaga atacacgcac gtggcgattt
    18301 gaattcacgc cgacccccgc gctctccgaa cgcggcgtca acgtcaacag cattcgcgcc
    18361 cgtctgcaag aggtcggcga aattttgcga gccgccccgc acgtcaggga gcagggcaga
    18421 atcatcttcg agttcgtcgt cgccaccgtc gtcgaagaat cggcgttcgc gggctggcac
    18481 gaagacaacc tgacatacgc gccggtcgga gaagaagccc cgagcacgat gcacacgccg
    18541 tcgccgccga cagggtcggc cccggcttcc gacactcgcg aagtcgaaga ctcgcggccg
    18601 accccggcgg cgcccgccgc gtcgaggcgc gcgccctcca acgtcgtgcg ggtagacctt
    18661 gcgcgtctgg acgaactgat gcggctcatc ggcgagctgg tcacgagccg cgcgcggctc
    18721 gacgacggcc tgaagcagtt gcagcgtcac gtcgcccccg ccgagtggcg cgggttgcag
    18781 gagacgagtc atgcgatggc ccgtcaactg cgcgacctgc gcgaaggcgt catgcgcgtg
    18841 cggatggtgc aggtcggcga aatcttcgag cgcatgaggt tcgtcgtccg cgacctggcg
    18901 cgcgagagcg gcaaagagat caagctcgaa cttctggggc aggagacgga ggtggataag
    18961 ttcctcgtcg agcgcatgat gaacccgctc ctgcacctcg tgcgtaacgc cgtcagtcac
    19021 gggctggaga cggtggagga acgcgccgcg ctcggcaagc cgcgggaggg aaaaatcacc
    19081 ctgcgcgccg cggcctcggg cgacaacgtg ctgatcgaga tcgcggacga cggacgcggc
    19141 atcgacgcgg agcgcgtcgc cgcgcgcgcg cgcgagcgcg gcctgattgc cggggatgtc
    19201 ggtatcgacg agtcgctgtt gttggagttg atttgcgcgc cgggcttctc gacgcgcgac
    19261 gaggcggaca gggccagcgg gcgcggcgtc gggatggacg tggccgccaa aaccatcgag
    19321 gaactcggcg gcgccctcac gctcgcgacg gagaagaacc gcgggacgcg cttccgcgtt
    19381 gagctgcctc tgacgctcgc catcgccgac gcgctcatcg caagcgttgc cggacagcgc
    19441 ttcgccttcc cgcagacggt cgtgcgggag gtcatcgaag tcgaggcgtc ggcggtgaag
    19501 gttttagaga acaacgagat cgtttcttac cgcggcgggg tgctgccgct ggtgcgcctc
    19561 gcgcgcctgt tcgggctgga cgagacgacg ccgccgcggg ccttccacgt cttcgtggtc
    19621 gggagcggcg cgggcgccat gggtcttgcg gttgaccgcg ccgtcgggca gagggagatc
    19681 gtggtgcgcg gcatgaacga ccctctggtg aaggtcgcgg gcgtcgcggg cgccaccgac
    19741 ctcggcgacg ggcgcgtcgt gcttatcctc gacgtggccg ccctgaaata ttcggcgccc
    19801 gccgtcaaac attccgccgg gaacgctaag gcgcgtcttc cgcggaagag ataaattcat
    19861 gtcgcataca cagaaggatg ccgagccctt cgttctcttc gagctggccg ggacgactta
    19921 cggcgtgcgc agccgcttcg tgcagcagat ggagatgatc gagcacatca cgccggtgcc
    19981 gaacgcgccc acggcggtcg agggcgtcgt gctcgcgcgc ggtcaggtca tccccgcgct
    20041 caacctgcgt gtgcgcttcg gctttgagag gattcctttc gacctgcgct cacgcctgat
    20101 ggtcgtcaac cagggcgagc gcacgctcgg cctgatcgtg gacacggccc gcgagtttct
    20161 gcgcatcccc gagtcggcca tcgagccgcc gccggaggcg atcacagggc tgagcgggaa
    20221 gtatctcgaa ggcatcgcga cgattaaaga gcggatgatt ctagttctca atctggccga
    20281 ggtgatcgac atcggcgaac agcagcccgc gtcggctgaa gggttaatag gagacggaca
    20341 tgtcaacaca gactggtaat gcggcggtcg gcggactcgg cgtgaacgcc gtgcgggcgc
    20401 gcgaacacat ggcgcaggtg gcggacgcgg cgcacgagat agcgcgcatc gcgcgtgagg
    20461 tgaccgaagg ggcggacgcg cagatacact cgctcgactc ggccgtcagc ctcgcgaatg
    20521 agacgacggc ctcgctcggg gagacgacca cgcaggccga gtcgattgcg acttcgacgg
    20581 aagaactcgt ctcctccgtc aacgagacgg ctgcctccat cgagcaggtc agcgcaaact
    20641 cttcgagcct cgccgcctcc atcaacgaga cggcggccgc catcgaagag tcggcccggt
    20701 ctatcgagag cgtcaccgcc aacgcgcagg agatggcgac ctccgccgcg gaggtgacgg
    20761 gcgcgatgaa cgagatcgcg gcctccgtca agagcgtcag cgacgacacg gaggcgctct
    20821 ccgtgtccgt caaccagacc gccgtcagca tggagcagat gtcgcgctcc atcgacagcc
    20881 tcgcggggaa cgcagacgac ttgagcgtgt ccgccgagca gacggcctcg tcgatcaacg
    20941 agatggccgc ctccatcgaa gaggtctcgg cgatgtccga gaatctcgcc tcgaccgtcg
    21001 aagaggtttc gacctcgatg gaagagatgg cgcgctccgt gcagggcgtc gcgcagaaca
    21061 cggagaagat tacggaggcg gcgacgggcg cggcgacgag cgccgccgag atggagcgct
    21121 cgctgcgctc ggtcgcggcg atgaccaagc gcgccgaaga ggtcgcgggc cgcgtgtcgc
    21181 gtgaggcgga gcagggcggc ggggcggcgc agaaatccat ccagggcatc gggcgcgtcc
    21241 gcgcctcgat ggagcagtcg gcggccgtca tccgcgagat gggcaagcgc tcgaacgaga
    21301 tcggcggcat cgtcaacacc atcaacgtca tcgccgagcg caccaatatg ctctcgctca
    21361 acgcgtcaat cgaagcggcg cgcgccgggg aggcggggcg cggcttcgcc gtcgtcgccg
    21421 aagagattcg caacctcgcc gaccgctcgg cgcaggcgac cgccgacatc gccgccatca
    21481 tcaaggcgtt gcaggacgtg gtgcaggagg ccgtcgcggc gtcgagcgaa ggtgtgcgcg
    21541 tcgcggaaga gagcggccgt ctcgcggagg aggggtcgga agggctgcaa agaatcctct
    21601 ccggcgtggg cgagaccaca cagatcgtcg gccagatagc gcgcgccacc gaggagcagt
    21661 tgggcgcggg agcgcacgtc ttagcgtcga ttgagacgac cgcggcgcag gcccggcagg
    21721 ccgcggtcgc cacgtccgag caggacaagg ccgcacagga gattgtcaaa gcggcggcgc
    21781 agatgcgcaa gaccgccaag gaggtcagcc aggcgatgag cgagcagggg cgagccgctc
    21841 gcgagatcat caaggccgcg cagagcacga ccgccgtggc cacgcaggtg cgtaaagcct
    21901 ccaccgagca ggcggcggcc gccaaacagg tcgcgcaggc ggtcgagtcg atgcgccgcg
    21961 gcgcggcctc cacctcgcgc gcgctcgaag agcaggccac ggcgggcgaa caggtctcga
    22021 aggagtcgga acgactggcg cggatgatcg gcggggtaag ccgcgcgatg ggtgaacagg
    22081 cggccgccgc gacgcagatt accaccgcgg cgcagagtat gcgccagcag gccgatcagg
    22141 tcgccaaggc gttgggcgag cagtcccgcg ccgcacgcga catgaccgcc gcctcgacga
    22201 gcatctccaa agagatcggg ctcatcacgc gctcgaaccg ccaacactta acctcttccg
    22261 agcaggtctt aggcgtgctg accgacattc gacagatcac cgagcgcaac gcgcaggggg
    22321 tcaaagccac actgagtttg acggtcggtt tgatcgagcg ggctcagcaa ctcaccgcgg
    22381 ccgtgggcga cgcggacgcg ggtcaaacgg gcgaacgtgt cgcgccgtcc gcaagcgccg
    22441 caccacgccc taacggaaga ccaagacgct cgaagaaggg cttgactccg ggcgaagccg
    22501 cgggcaacgg cgggccggag cagtccgacg gctccggtca atgaagacaa ctttcgtttt
    22561 cgggcggtaa gtattcggcg gggactttga cgcaccggga tgggtagcct gactccttca
    22621 gacaatgcaa tcggcgtctt cacgaccgac gcgagtctcg ttattcaaat ctgggacgcg
    22681 gccctcgcac gcctgacggg catccccgcg gacgccgcgc gcgggaaaca tctcgccgaa
    22741 ctcatcccaa atttagaaga gggcagactg ctcgcgcgtt tccggcgggt gctggacgaa
    22801 ggggtcgtcg aagtcctcgc gcccgccttc catcactact taatcccgtg cccgccgtcc
    22861 gccccctcga agcatttcga caggatgcag cagcgcgtga ccatcgcgcc cctgcgcgag
    22921 gacgacagga cggtcggcgc gctcgtcacg gtcgaagatg tcaccgcgcg gatcgaacgc
    22981 gagcacagcc tctccgagca acccgcacgc gccgacgaag gggtgcgcct cagcgccgcg
    23041 cgtgcgctca aggaggagga gtcgctcgaa cccgcgcggc ccctcctcgc cgcgctcggg
    23101 gatgagaact ggcgcgtccg ccgcacggcc gtcgagggac tcgcgcgccg cgccgcgccc
    23161 gacgccatcg ccgcgctctt agcttcggtg cgcgagaatc atcaaaatct cggcctcctc
    23221 aacagcgcgc ttcaggtgct cgcgatgagc gacgtggaca cgctctcgca gctcgtcgaa
    23281 tttctgaacg acgaggacgc agacctgcgc atgcaggcgg ctctcgcact cggcgagcag
    23341 cgcgacgggc gcgccgcccc cgcgctcctc ggcgcgctcg aagacgcgga catcaacgtg
    23401 cgttaccacg caatcgaagc attggggaaa ctgcgcgccg gcggggccgc cgacgcgctc
    23461 gccgggattg ccgagacgcg cgatttcttc ctggcgttcc cggcgctcga cgcgttgacg
    23521 cgcatcggcc aggcgcacgt cgctccgcgc gtcgtgccgc tgctcgaaga cgagctttta
    23581 cgcgaggcgg ccgccgacgt gctcgggcgc ctcggcgacg aagagacggt cgcgcctctc
    23641 gccgcgctgc ttaacacgcc gcacgcgccc gcgccgacag tcgcgcgctc gctcgctgcg
    23701 ctgcacgacc gctacgagaa actgtacggc gagggcgcgc acatcgccga cctgacccgc
    23761 gcggccatca gcccgacggg cgcgcagaac ctgctcgacg cgctcaacga gtcggactcc
    23821 ggggaaccgc gcccgctcgc gctggtcgtc ggatggttag aaggcgcggc ggtcgagcgc
    23881 gctctgacgc gcctgctcgg gcgcgccggt gcgcgcggcg aggtggtcga agcgttggtg
    23941 cggcacggct cgcgcgtgac ggatttattg attgaacagt tggggtcgga agacctcgaa
    24001 atcagaaagt cggcggtcgt ggccctgggg cgcataggcg acgcgcgcgc cacgcccgcg
    24061 ctcctcgagg ccctcgaaga agacccggag ctggtcatcc ccgcggcgga cgcgctggcg
    24121 aagatcggcg acccacgagc tttcgacggg ttgctcgcac tcgtcggcga cccacacgcc
    24181 gcggtcaggc aggcggccgt cggcgcgctc aactcgctcg gctcgcctga gatgccgggg
    24241 cgtgtgctgc cgctcctcga agatgccgac cccaacgccc gcgaatcggc ggtgaagatc
    24301 gccggctact tcggctatcc cgaatgcgcc gaccttctgt tggaacgatg ccgcgacgag
    24361 gacgagcgcg tgcgccgcgc ggcggtcgaa caccttccct tcctcgaaga cgaacgcgcc
    24421 acgcccgcgc tcgtgcgcgc gctgcgacac gagacgccga aggtgcgcgc cgcggcggcg
    24481 ggcgcgatgg cgctggccga aggccccgaa gtctcggctt ctttgattga agcactcggg
    24541 gacgaagatt cgtgggtgcg ctatttcgcc gcgcgttcgc tcggccggct gaagattgcg
    24601 ggaagcgcgg acgcgctggc ggggctggcg cgcgacgaca aagcgaatca cgtccgcatc
    24661 gcggccgtcg aagcgctggg cggcctcggc ggggagacgg cggtcagggc ggtcgccccg
    24721 ctgctccagt cggacgagcc ggacatcgtg cgcgtagcca tcgccgcact cggccggatg
    24781 gagaaccccg ccgcccggcc gccgctctta gaagctctgc aatcgccgga cgcagaggtc
    24841 agggctgccg ccgccgaggc gctcggggtg acgaaaagaa atcggcaggg agattctaac
    24901 tgacggcgga agggcctccg gccggtcgcc gcgggctgaa gaacaatggg ctttcacacg
    24961 ggtagtttag gtgtcacggg cggggcggac aggttgttgc gcgacctgat acacgagcgc
    25021 acggggctgt ttttcgataa cgggaagggc gacctcctga cggacaagtt gtcgccgctg
    25081 gtcgtcgagc gcgggttcac gtccctcctc gattattact acctgctgaa gtacgacgag
    25141 gcgtcgcagg acgagtggct gaacgtgatg aacgcgctgt ccgtccccga aacttacttc
    25201 tggcgcgaga tggatcaggt gcgcgcgctc gtcgaatggg tggtgccgcg ttggttcgcg
    25261 gcgccgggcg ccgggccgct gaagatttgg agcgcggcgt gcgcgacggg ggaggagccg
    25321 ctgaccatcg cgatggcgct cgaagaggcg gggtggctcg cgcgcgcgcc gattgagatt
    25381 cacgccagcg acgccagcac ggcggccgtc gaaaaggccc ggcgcggggt ctatcgcgag
    25441 cgctcgttcc gcaacctgcc ggcggggctg cgcgccaaat attttacgca gatgggcgcg
    25501 acacaaacgt ggcgtgttga cgctaggctg catgacaccg tcaagtgggg tgtcgttaat
    25561 ttgatgagcg aggcggagtc cgcgccgtgg gcggcggcga acgtgatctt ttgccgcaac
    25621 gtgttcatct acttctccga ccagacgatc agcaggacgg tgcgctcgtt cgccgcgcac
    25681 atccggccgc cgggttacct gtgcgtggcc gcctcggaat ctctgttgag gctttcggcg
    25741 gatttcgagt tgcaggagat cggtaacgcg ttcgtgtatg tgaagcggcc gggtcagggc
    25801 tgacggccgc ctcgcgcccc gaggactatt catggatcga gttctaaaag ttctggtggt
    25861 ggacgattcc gcatacgtgc ggaaggtcgt gaagcagatg ctctcgcgca gcccgttcct
    25921 cgaagtggtg gggacggccc gggacgggcg ggaggcgctg gagctggtgg tgcagttaga
    25981 cccggacgtc gtcacctgcg acctcatcat gcccgagttg gacggggtcg ggttcgtgcg
    26041 cgaacagatg gcgcgccggc ccgtgccgat catcatcatg agcatcgcga gcgagacggg
    26101 cgaggccgcg ttgaccgcgc tcgacgaggg cgcagtggat ttcgtacaga agccgacggc
    26161 gctggcgacc gagaaaatct tcgaggtgag tgacgagttg atcgagaagg tcaaggccgc
    26221 cgcgagcatc ccgatgggcc gcgtcaagaa ggccccgtca gagcctgcgc ccgcgccgtc
    26281 gcgagccttg gcctccgtca cgcgcaaaaa gctggtggac gtggtggtcg tcggcatctc
    26341 caccggcggc ccccaggctt tgaaacagtt aataccccaa ctccccgcgg atttccccgt
    26401 cccgatggcc atggtcatgc acatgccggt cggctacact gaaatgtacg cgcgcaagct
    26461 ggacgagcat tcgccgctcg aagttcgaga agcacaggag ggcgacgagc tgcgcgcggg
    26521 cctggcgctg ctggccccgg ccgggcggca cctgaccttc cgccgtcagg cgggcggcgc
    26581 ggtggtcgcg catctggatt cgaagccctt cgagatgccg caccgcccgt cggtggacgt
    26641 gctctttcag tcggcggccg aggtgtacgg gcagagggtt ctgggggtgg tgatgacggg
    26701 gatggggtcg gacggcaaac agggcgcggc ctggatcaag gcacaaggcg gtctggtcta
    26761 caccgagagc gaagagacgt gcgtggtcta cggcatgccc tcttcagtgg tggaggcggg
    26821 cttgagcgac cgaagcgtgc cgctcgacgg gatggcgcag gcgattcggg aggcggtatg
    26881 agtaaaaaaa taatggtggt ggacgactcc gcgctgtcgc gcagaacctt acgccgcatt
    26941 ctggagacgg ctggctacgg cgtcgtcgag gccgaggagg gcatggccgc gctcgaactc
    27001 tatttcatcg agaagcccga tctggtgctc ctcgacctgg tgatgaaggg catgtacggc
    27061 ctcgacgtgc tcgaaaagct gcgccagatg gatcccctcg tgcgcgtcgt ggtcctttcc
    27121 gcggatatcc aatcgacgtc gcgagagatg gtcgcggccg ccggcgccgc cgg
//
LOCUS       DASVES010000508        23685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1669326, whole genome shotgun sequence.
ACCESSION   DASVES010000508 DASVES010000000
VERSION     DASVES010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23685
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669326"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1792
                     /locus_tag="VF240_21515"
     CDS             347..1792
                     /locus_tag="VF240_21515"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX7177853.1"
                     /translation="MKLKDSRIIAVIVALIALLHFALSPREVKQVSPARTQTTESSRA
                     ARRAIVISLDGLDVRYLRRADEFSLKIPTLRRLMKEGVLAGVESVYPSVTYPNHTAIV
                     TGARPDRHRIVNNQVFVPPTSPQRGVQFWFARDIKTETLWDAARKRGLSTGLVSWPVS
                     TGAGDWNVPEIWKPGTSPSDSFPTTLAEISRHARPAGLVEEIAERVPDIYKNVTKDEG
                     DDHRAHWAEYIIREKRPDLMLVHLFDLDHTEHNRGPFTPEAFAILEKVDGYVGRILAA
                     AEQAGTLSETAVFIVSDHGFRDLSKYIHPAVVLREAGLVTTRDERSANVQTQTVVTDW
                     RAYPYPSGGSCAIVLRDAGDRDAAERARAALEKFNEQQGGKLFRIVGRDELSKLGAFP
                     EAAFGLEATFGYAFGDNHSGEAITETTRRGTHGYLPMPADYRATLVVSGLDVVRRGDL
                     GDVHMTEVGPTIAAALGLTLGDAQHGALKLR"
     gene            complement(1830..2435)
                     /locus_tag="VF240_21520"
     CDS             complement(1830..2435)
                     /locus_tag="VF240_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177854.1"
                     /translation="MKRRGLSGLLCALCCVAGLTFSATPAKAQGSRYVGRDIVGTVVG
                     VGGRLGGRTRQFRLIVERYTSPQDVQRLTSALQTGGQDELLSVLSKLNAGRIQIGTGV
                     GITANAIIPTELPEGGTKLVVLFQRNINFYELRHGTRSEDYKFGYAELHLGRKGDNEG
                     TFIAAAKIKLEDGNSWVVEDFGEFPARLLGLQARGGKGGAR"
     gene            2822..5416
                     /locus_tag="VF240_21525"
     CDS             2822..5416
                     /locus_tag="VF240_21525"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7177855.1"
                     /translation="MKECPACNRCFPDNVNHCPSDGDALRATITGDTVLDSRYQLEKR
                     LGQGGMGVVFKGRHVYLKTTHAIKIILPDLVGNDPSLITRFRQEAMAAANIRHQNIIS
                     VTDFGVVNDTMPFLVMELCEGRSLHDILAEEKRLPAARAIEIMLAVAAGVGAAHNQGI
                     VHRDLKPLNIMLKDGCSAAEGVKVLDFGLAKIKSGDLLGSFVAAQTQGLMGSPFYMAP
                     EQWSDEEPDARADIYALGVILYQMLAGEVPFRGNSIPSIMKKHLGEPPPAFTEKGVEV
                     PPEVERVVRHALEKEAEKRPQAVVELSLELLQALSAVSAPAAPSAVSQVASSDFGMKA
                     EAPAGQGIVQPDPVRAGGQPTGELAARGGVTSVDVGRQADAPPDFEESAPAVTPRVES
                     SPRIEYNEGRETSHDIAAGATAVIPDAFIERDDSLSPMPSTPTSSPSAPLPTSSASPP
                     PLQTSTPSMPPASQSPPTAGPEASSSGFAESPSFYAAPPFTSSETQAFATTPLSAERL
                     SQFQESSQTTIEPNVSERVEALPPVVASPPGSQRVGTMAAPTGSRVPVTVWIAGGVLA
                     LLFVVAGLYFALKPTQSDDETNTNLRANVNATPATNRGGGGNANVAATPAPGSAPDLV
                     ALPGGSFMMGRNDVPPRTSEVSVAYQAWVYNQWPAHSVNVSAFAIDRTEVTNAEYAEF
                     VEKTNHAPPAEWGGRRAPAGQEKWPVGGVSLDDAERFARWRSERDKVQYRVPTEAEWE
                     FAARGGAGAGARLYPWGAEWADGRANLETDALRPVGSFPEGATPQGALDMIGNVWEWT
                     ATQAGMYRGNNLLVLASGDRGKFVVRGGSYQSKSRGDEPVTATARRWVPRAERNALVG
                     FRLVRPGK"
     gene            5427..6008
                     /locus_tag="VF240_21530"
     CDS             5427..6008
                     /locus_tag="VF240_21530"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7177856.1"
                     /translation="MNERADTSPRQGFWTYTRLALAALAFFLIVIAVSTGLRAPQAPA
                     TAKDSLPPSVLGTELRALDGPAFRLSDYKDRVVVLDIWATWCGPCRLEIPHLVRLSEE
                     YGPRGVEVIGLSVEDPASAGGAVRDFAREFGINYRLGWAEQRWTNTLTRGAATIPQTF
                     VIGRGGRRLLHLQGYTPQISATLREAIDRALAE"
     gene            complement(6134..6379)
                     /locus_tag="VF240_21535"
     CDS             complement(6134..6379)
                     /locus_tag="VF240_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177857.1"
                     /translation="MEIDKDKIVTQEETGEPADIDAVEGRVEAEMKRIEGKAKERVAQ
                     GLGDAELAEEARDLKAEGERELQEEREKEREKEAEKD"
     gene            6585..7049
                     /locus_tag="VF240_21540"
     CDS             6585..7049
                     /locus_tag="VF240_21540"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HEX7177858.1"
                     /translation="MTEDITGLLRHGHDTIVRSWAEKVTADRRVRTDAQLTYLQLLDH
                     VPDIVEELREALASGERPDGQLLEEARNHGRTRWQQGYELKEVVRELTLLRAAVVEFI
                     DAYRGALATPPPDLVRSYNRIAGFMDEEIYRTIEAYLEAHREAIATGGISVN"
     gene            7184..7684
                     /locus_tag="VF240_21545"
     CDS             7184..7684
                     /locus_tag="VF240_21545"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7177859.1"
                     /translation="MLKTASFSGEDRGRSALRIGEVAKASGLGVETLRFYERRGLLGR
                     PRRTAANYRVYDDSVLERLAFIRRAQAIGFSLDEIQTIIAESEDGRRPCGHVRELARR
                     KLSELDERLRELRAHRGELARLLGEWDERGEEAGQFCGLIEHSRIEARKAPVRSLRAG
                     KNGGRR"
     gene            7681..10017
                     /locus_tag="VF240_21550"
     CDS             7681..10017
                     /locus_tag="VF240_21550"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7177860.1"
                     /translation="MSLTAEKELTRSPERIDLPITGMTCAACARRIERALSKAQGVAS
                     AGVNFATERATVEFDAGQTDEGALRRVVEEIGYGVRELPRGRGAGVEEDAEEKSRSEE
                     RRELRRRFVVAALLTLPVVVIAMSHGRLPLTDGPWVNYLQLVLTGTVVFYAGWPFLRG
                     AWTALRHRAADMNTLVATGTLAAFLYSVVATVAPQLVTTATHAGMPAATGEMSMGSGT
                     APMAPVYFEAAAVIITLILLGRLLEARARGRAGEAIRKLLGLQARTARVVRDSGATED
                     VPVEAVVPGDVILVRPGEKIPTDGRVVEGASAVDESMLTGESMPVEKAVGDSVVGATL
                     NRTGSFKFRATRVGSDTVLRQIVRVVEEAQGRRAPVARLADTVSGYFTPVVIVIAIAT
                     FVVWFIAAPVETRLATALVNFVSVLIIACPCALGLATPTAVMVGTGRGAALGVLIKGG
                     EALETAHKVQTVVLDKTGTLTKGEPGVTDVAAAEGIAEDELLRLAAAVERASEHPVGA
                     AVVRHAEARGLATASASDFGALAGHGVEASVEGRRVLVGNLKLMNERGVSLGSLEARA
                     DEFAAEGKTPVFVALDGRAAGVVAVADRLRPESREVVAALRALGLEVLMITGDNRRTA
                     HTVARELGIEGVLAEVLPEGKAEEVRKLQAAGRVVAMVGDGINDAPALVQADLGIAVG
                     TGADVAIEASDVTLVGGSPRGVLTAIALSRRTMRVIRQNLFWAFFYNALGIPLAAGLL
                     YPLTGWLLSPVYASAAMAFSSVSVVANSLRLRRFGKGQ"
     gene            10117..10497
                     /locus_tag="VF240_21555"
     CDS             10117..10497
                     /locus_tag="VF240_21555"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7177861.1"
                     /translation="MDTQEIIVLAGGVLLAGLVVWFFFMSEGARASAEVVEGGVQRVR
                     VTVKGGYSPDVVVVRKGVPVELDFYRDETSSCTEEVVFGDFQISRRLPAFETTTIRLT
                     PERAGTFTFNCGMNMVRGKLVVED"
     gene            10612..11127
                     /locus_tag="VF240_21560"
     CDS             10612..11127
                     /locus_tag="VF240_21560"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HEX7177862.1"
                     /translation="MKIKLLSLALFALAASAACGAGDQQRAVKQVNAPPKVEQVNAPP
                     KVEAAHAQKKPADDHGHDHGEPGRVPAFESNPKNLPATLAPEAFTGKTRQAYRAVREI
                     PETIAQLPCYCHCDRGFGHKSLHSCYVDGHASSCAVCVEEVLTAYRLQKEEGLSPAQI
                     RERIIAEYSKL"
     gene            11138..11866
                     /locus_tag="VF240_21565"
     CDS             11138..11866
                     /locus_tag="VF240_21565"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX7177863.1"
                     /translation="MRKLFATAVAALALCLCAADAASGQGTKGHRATRTKDASRHSKA
                     AADAFNSIMRVRDKAIPKDLLENAEAVAVFPGVLKAAFIIGGRKGQGVISRRTPQGWS
                     APVFYNMSGGSIGFQAGGSKTDYVLLIMNEDGLKGLLEDKFEIGGEAGVAAGPVGRTI
                     SASTTPTLDAGILSYSRSKGVFAGASIKGVAITPDNDLNRAYYGRTAREVLTSGQRPP
                     TLVRSLPLAISRYSNRRAAPAPKK"
     gene            11896..12654
                     /locus_tag="VF240_21570"
     CDS             11896..12654
                     /locus_tag="VF240_21570"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX7177864.1"
                     /translation="MNRSRRLSAALTVSLFLIVASFVAPAGAQNERPRKAETPAARPS
                     AKQLKEAEKKARAAAKVFDEIMDAPDSSVPRELLDRAEAVAVFPGVLKAGLLVGGRGG
                     SGVISRRIAGGWSAPAYFKLTGGSVGLQIGAAKTDLVLLFMNEPALKGLLEDKFEIGG
                     EASAAAGPVGRAASATTNVTLDAGLLSYSRSRGLFAGLELKGAWINPDNNLNEALYGL
                     KARDLLTGATKINMADVPAGVIVFPNALGRYSIK"
     gene            12862..13485
                     /gene="lexA"
                     /locus_tag="VF240_21575"
     CDS             12862..13485
                     /gene="lexA"
                     /locus_tag="VF240_21575"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX7177865.1"
                     /translation="MQPRTRRQKEVLDYITRFIERHGYEPSYAQIARHFRVSSKATVA
                     KHITSLERQGLLTRRRDDGGFGLSVHVEEVPADATCEVPLMGRVAAGAPLDTVEERET
                     IVIPRFLLGRTRPGQVYALRVQGDSMIDEHICDGDIALVENRTEARNGEIVVALVDGQ
                     RATLKRFHRAGNDVELKPANSNLEPIKLHASRVSVQGIFRGLLRPAS"
     gene            13705..14793
                     /locus_tag="VF240_21580"
     CDS             13705..14793
                     /locus_tag="VF240_21580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HEX7177866.1"
                     /translation="MSERTTKKVAILIEQHVEDSEFQIPYTALQKAGAEVTVLGSRVN
                     ETYAGKQAKVSIKADATTTDARAADFDAVIIPGGMAPDKMRTNMKTVRFVRDAVEQGK
                     LVAAVCHGPQVLIEADVLRGKRATGFRSIRKDMQNAGADFENEALVVDGQLLTSRRPS
                     DLPVFTAAIIRRLGLELKDVSLPEEGVAYDDWWKLAEEWGGSTKDEIVDGLNTAIRGE
                     RYALSAFEQYVLKATAPDIKSLFGEIVASKQRHIQSLEARLAAVGGKEDLQAGGAGAW
                     ATLKSWLQSSDDVSLLRRALGDLQTGVVDAFNLTNSFTDPATAAVFDQIEIDLAENEQ
                     RVADLLHARTSGQESQPATPSTGAAVSA"
     gene            15250..17319
                     /locus_tag="VF240_21585"
     CDS             15250..17319
                     /locus_tag="VF240_21585"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7177867.1"
                     /translation="MGAIYMSRIDNSRTAGRTLASLLLLISLTSSLCAGVAAGARKPQ
                     RRPRISAPASYLSASHVASALSSSADTISDPYGKLTTDVQKEAGKKGWDGNDKTRVIV
                     QLRDAASARLLALLASGGASVRAQHQSLEVLILDVPVRLLPALTHFREVDSISTDREL
                     KPSGHVTATTGLDEAAAASSYGKLDAAGVGIAILDSGIDESHPSFAVKGDGPSRVRLS
                     LDFTGEGRTDDPYGHGTHVAALALGNRAVGEGKYAGIASNADILNLRVLDSTGAGTVS
                     SVLSALEWLYANRTKYNVRVVNLSLGAPAVTSYRLDPLCRAARRLVAAGIVVVAAAGN
                     EGKDRDGLKVYGAVHSPGIEPSVITVGATNTFDTDSRADDAVATYSSRGPTRGYWTDA
                     LGVRHYDNFVKPEIVAPGNRLVAAKARDNYLVRTYPHLDATRAGGAAMMYMSGTSMAA
                     PLVAGAAAVLVKVNNALTPNLAKAILMYTSQPLAGYNQFEQGAGQLNVEGAARFALGV
                     RKDFASQLLGAPLQTTHEVEGGLVKALSDGLNGASAGAIEPRTNISGHGFAWSQTLVI
                     NHAVVFGGELLTRCQNVYGGGIMMSDAVIESGVTQSINRTVMTRGIMMSDQIVTSDGS
                     PQGGGLPFLSTGILMGDGIMMSDGILMGDGILMGDGILMGDGILMGDGILMGDSHLDG
                     DDTAPVQ"
     gene            17348..19603
                     /locus_tag="VF240_21590"
     CDS             17348..19603
                     /locus_tag="VF240_21590"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7177868.1"
                     /translation="MSNLHTIRRGASLTLTLILLGGALAGGTRLVVPAGAQSGPGGSS
                     GSSGSSQKIKVGADLRRKAKEAPALRVNVIVQPAGAWTVSLDSDLKGKDAAVRDARQN
                     FSARSVEMYASEVENIANRDDVAYVSLDREVKKLGHVSLTTGADAARSMGGSTVYDGA
                     GIGIAVLDSGIDPNHVAPKNESLSVSRVAASVDFTGEGRTTDPYGHGTHVASIAAGNG
                     KVASDAYRGIAPAARLLNLRVLGADGRGTTSSLLNALDWVMTNRAAHNIRVVNLSLGT
                     AAVDSYLNDPLCLAVRRLVDAGIVVVAAAGNEGKDASGNKIYGQIHSPGNDPSVITVG
                     ASNSFGTDTRSDDAMTTYSSRGPTRSFWIDAQGVKHYDNLIKPDLVAPGNKIISAQAA
                     GNYLVSSNPVLDADVSGNAARDQMYLNGTSMATPVVAGAAALLLQANPSLTPNLVKAI
                     LMYTSQPLAGYNQFEQGAGQINIEGAMRLARLVRTDLTSATPVGTPMLTGAAPAAQTT
                     IAGYTFLWAQGLILDHTFVTGQNLITQYQGIYGTGVMLSDATLLSSGVLMGDGVMMSD
                     GVMMSDNILTSAGITFSEGTIFIPTNRLYCDGVLLSDGVLMGDGVLMGDGVLMGDGVL
                     MGDHVLMSDIASQAGAAIISGDPGPLMGVVKETLPGAPLSLAVSAVSSSQLKLTWKDG
                     SANEDGFQLERCAGSNCTNFAQVATVAANTITYSNSGLTKNTTYRYRLRAYNGAGYSS
                     YTAIVSKATPR"
     gene            19971..22448
                     /locus_tag="VF240_21595"
     CDS             19971..22448
                     /locus_tag="VF240_21595"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7177869.1"
                     /translation="MYRPRLSKPFMWLTIAAGAAVCLHAAREIAAGQLDYRFLLLAAA
                     TLGIGSRISIKIPHDKGQISVSDTFIFLAILLFGPGPAVLLAATESFCSSLRFCKHRV
                     TVFFNAAVMAVSTFLTVYLLQLCFGSVADLRGGYSAVYIVGICFVGFVQYVLNSGLIA
                     LGYALKNDQPLWTTWHKHFLWTSVSYFAGASAAGFITRLIDASGFYALLATAPIVAIV
                     YITYTTYLKNVEVAQAQAEQAERHMGALRESEERFRSVFDQAAGMALVSAEGRWVRVN
                     RSLCEMLGYEEHELLATDFQATTHPDDLGPMLGEVARLLEGRIDRFQFEQRFLHRRGQ
                     EMWALLSVSLVHHAQAATHELIFQIQDITDRKRAEERLIHDAFHDVLTGLPNRALFID
                     HLKLSVERAKRRDKRLFAVLFLDLDRFKVINDSLGHMVGDQLLIGIARRLETCLRPGD
                     TVARLGGDEFTVLLEDLTGPGEAIEVAERLQKELCLPFNLNGNEVFTTVSIGIALSTT
                     GYERAEEVLRDADTAMYRAKTLGKARHEVFDKAMHTRALNLLQVETDLRRAVERDEFV
                     LHYQPIVSVGTGEVRGFEALIRWQHPERGFVSPMDFIPIAEETGIIAQIGKWVLEEAC
                     RQARAWQKKFPAHPPLQMSVNLSGKQFMKSDLIGQVRAALAATGLDPRTLKLEITESI
                     MMENIETAAETLGQLRALGLQLSIDDFGTGYSSLSYLHRFPISTLKIDRSFVGRMDAT
                     GDNAEIVRTIIMLARNLDMDVVAEGVETEGQLTQLKALGCEYAQGFLFSRPLDARAAE
                     SLLEEHESSRRAAPALDWPAVIVTRDM"
     gene            22558..22959
                     /locus_tag="VF240_21600"
     CDS             22558..22959
                     /locus_tag="VF240_21600"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX7177870.1"
                     /translation="MDDTAFEVKPSRIHGRGLYARALVPARRKMGELGGERITRREAR
                     RRARESDCITIVEFEDGTALDASSGGTHFRYVNHSCTPNTFMRCIRGRVEFYALRQIN
                     PGEELTCNYGETHHDGALPCRCGSARCRGSI"
     gene            complement(23183..23258)
                     /locus_tag="VF240_21605"
     tRNA            complement(23183..23258)
                     /locus_tag="VF240_21605"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23223..23225),aa:Thr,seq:cgt)
     gene            23336..>23685
                     /locus_tag="VF240_21610"
     CDS             23336..>23685
                     /locus_tag="VF240_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177871.1"
                     /translation="MPKDPTRNVDRYKVRGGQFDDLDRTKNDGVMTEDVAPLTGGPEE
                     TNFIPGEPAQDAAERKQRLIEEVHAKAEQRREAEAAKARKSARAARGKTVAKKSAKKS
                     ATPSVKKAAKKSAK"
BASE COUNT         4105 a         8298 c         7509 g         3773 t
ORIGIN      
        1 aaggagatcg tcaaggtggt cgtcgtcccg aagcgtctgg tcaacatcgt cgtgagataa
       61 tacggcgggg cgggaaccgc gtgcgggcgc ccttcgttct acattgcgac ccctgatttt
      121 tcgcgccgcg cgcgttatag atcatttcaa cttgagtgtt aggtatggcg gctcaccgcg
      181 gaggcacaga ggacgcagag actacgccgc aggcttgacg cttccgctcg acgttgaatc
      241 tgctcaaccc tctgcgcttc ctctgcgtcc tctgcgcctc cacggtgagt ccaacgcccc
      301 caagactcga ctgaaacaag ggtaaaatct tttcgaggtc gcccacatga agctcaaaga
      361 ctctcgcatc atcgccgtca tcgtcgccct cattgcgctc ctccacttcg cactctcccc
      421 gcgcgaagtc aagcaagtct cgcccgcccg gactcaaacc acggagtcgt cgagggccgc
      481 gcgccgcgcc atcgtcatct cgcttgacgg gctggacgtg cgctacctgc gccgggccga
      541 cgagttcagc ttgaaaatcc cgacgctccg gcggctgatg aaggaagggg tgctcgcggg
      601 cgtcgagagc gtctacccgt ccgtgaccta cccgaaccac acggccatcg tgacgggcgc
      661 gcgccccgac cgccaccgca tcgtcaacaa tcaggtcttc gtacccccga ccagcccgca
      721 gcgcggcgtc caattctggt tcgcccgcga catcaagacc gagacgctct gggacgcggc
      781 ccggaagcgc ggcctctcga cgggcctcgt ctcgtggccc gtctcgacgg gcgcgggcga
      841 ctggaacgtg cccgaaatct ggaagccggg cacaagcccc tccgactcct tccccacgac
      901 gctcgccgag atcagccgtc acgcgcgccc cgccggcctc gtcgaggaga tcgcggagcg
      961 cgtgcccgac atctacaaga acgtgacgaa ggacgagggc gacgaccacc gcgcgcactg
     1021 ggccgagtac atcatccgcg agaagcgccc cgacctcatg ctcgtccacc tcttcgacct
     1081 cgaccacacc gagcacaacc gcgggccgtt caccccggaa gcgttcgcca tactggagaa
     1141 ggttgacggc tacgtcggcc gcatcctcgc ggctgccgag caggcgggca cgctttcgga
     1201 gacggccgtc ttcatcgtca gcgaccacgg cttccgcgac ctgagcaagt acattcaccc
     1261 ggccgtcgtg ctgcgcgagg cgggtctcgt cacgacgcgc gacgagagga gcgcaaacgt
     1321 tcagacgcag acggtcgtca ccgactggcg cgcttacccc tacccgagcg gcggctcgtg
     1381 cgccatcgtg ctgcgcgatg cgggcgaccg cgacgccgcg gagcgcgcgc gcgccgcgct
     1441 ggaaaaattc aacgagcagc agggcggcaa attgttccgc atcgtcggcc gcgatgagct
     1501 gagcaaactc ggcgcgttcc ctgaagcggc cttcgggctg gaggcgacct tcggctacgc
     1561 cttcggcgac aaccactcgg gcgaagccat caccgagacc acgaggcgcg gcacgcacgg
     1621 ctacctgccg atgccggccg actaccgcgc gacgctcgtc gtctccggcc tcgatgtcgt
     1681 ccgccgcggc gacctcggcg acgttcacat gaccgaagtc ggcccgacca tcgccgccgc
     1741 gctcggcctc acgctcggtg acgcgcagca cggcgcgctg aaactgcgtt agtccatgcc
     1801 ggcccgcggc gtcgcccagc gccacgcgct taccgcgcgc cgcccttgcc gccgcgcgcc
     1861 tgcaatccaa gcaggcgcgc cgggaattcc ccgaagtctt cgaccaccca actgttgccg
     1921 tcctcaagtt tgatcttcgc agccgcgatg aacgtccctt cgttgtcccc cttccggccc
     1981 agatgcagct ccgcgtagcc gaacttgtag tcttccgagc gcgtgccgtg gcgcagttcg
     2041 tagaagttga tgttgcgctg gaagagcacc accagcttcg tgccgccctc cggcagctcc
     2101 gtggggatga tggcgttggc cgtgatgcca acgcccgtgc cgatctgtat ccgccccgcg
     2161 ttcagcttcg agagcacgct cagcagttcg tcctgccctc ccgtttgcag cgccgaggtg
     2221 agccgctgca cgtcctgcgg cgacgtgtat cgctcgacga tgagccggaa ctggcgcgtg
     2281 cgcccgccga gccgcccgcc gacgccgacg accgtgccga cgatgtcgcg gcccacgtac
     2341 cgacttcctt gagccttcgc cggtgtcgcc gagaacgtca accccgccac gcagcagagc
     2401 gcgcacaaca gaccggatag tcctctcctc ttcatgtcgc ctcctgttaa cccgaacctt
     2461 tcaacctggg aacgtgaaag tatatgccca cgcgagaacg atggtctatt ccttctcgta
     2521 cttaaacgcc gccgccacac gaccgcccac tcgcccgcgc gcgcacacag cctccgacca
     2581 tattgcttac tgcctactgc ttactgctca ctgctcactg ctcactgctc acttccttgc
     2641 cttgcctcgc tctcatcgag ttgttagaat ttttcccacg tcgtggttgt cgtcgaagcg
     2701 tttccagctt cgaccttcgc cgccgcgcct gttcgcggcg attcgtttta ttgtgcagca
     2761 gacgagagtg tcgtgcgccg gacgttcgcg gtcgttccgt tcggcgcgct tgttgtttca
     2821 gatgaaagag tgtccggcct gtaaccgctg cttccccgac aacgtcaacc actgtccctc
     2881 ggacggcgac gcgctgcgcg ccaccatcac gggcgacacg gtgctcgaca gccgctacca
     2941 gttggagaag cggctcgggc agggcggcat gggcgtcgtc ttcaagggcc ggcacgtcta
     3001 cctcaagacc acgcacgcca tcaagatcat cctccccgac ctcgtcggca acgacccctc
     3061 actcatcacg cgcttccgac aggaggcgat ggccgccgcc aacatccgcc accagaacat
     3121 catcagcgtg acagacttcg gcgtcgtcaa cgacacgatg ccgttcctcg tgatggagct
     3181 gtgcgagggg cgctcgctcc acgacatcct cgccgaggag aagcgtctgc cggcggcgcg
     3241 ggccatcgag atcatgctgg ccgtggccgc cggcgtcggc gccgcgcaca atcagggcat
     3301 cgtccaccgc gacctcaagc ccctgaatat catgctcaag gacggctgct cggcggccga
     3361 gggcgtgaag gtgctcgact tcgggctggc caagatcaag tcgggcgacc tgctcggctc
     3421 cttcgtcgcg gcgcagactc aagggctgat ggggtcgccc ttctacatgg cccccgagca
     3481 gtggagcgac gaggagccgg acgcccgcgc cgacatctac gcgctcggcg tcatcctcta
     3541 ccagatgctc gccggcgagg tgcccttccg tggcaactcc atcccctcga tcatgaagaa
     3601 gcacctcggc gagccgccgc ccgccttcac ggagaagggc gtggaggtgc cgcccgaggt
     3661 cgagcgcgtg gtgcgtcacg cgctggagaa ggaggcggag aagcgcccgc aggccgtcgt
     3721 cgagttgtcg ctggagctgc ttcaggcgct ctcggccgtg agcgcgcccg ccgcgccgtc
     3781 ggccgtcagc caagtcgcga gttctgactt tgggatgaag gcggaagcgc ccgccgggca
     3841 gggcatcgta cagcccgatc ctgtgagggc gggggggcag ccgaccgggg agctggccgc
     3901 gcgcggcggc gtgacctccg tggatgtcgg gaggcaagcg gacgcgccgc ccgacttcga
     3961 ggagagcgcc ccggcggtga cgccgcgcgt cgagtcctcg ccgcggattg aatacaacga
     4021 aggccgggag acttcgcacg acatcgcggc cggcgcaacg gccgtgatac ccgatgcgtt
     4081 catcgagcgc gacgactcgc tttcgcccat gccgtccacg ccgacgtctt cgccgtccgc
     4141 gccccttcct acttcttccg catccccgcc gccgttacaa acttctacgc cctcgatgcc
     4201 gcccgcgtct cagtcgccgc caacggccgg gccggaagcg agcagctccg gcttcgcgga
     4261 atcgccgtcg ttctatgccg cgccgccgtt cacatcgtcg gagacgcagg cctttgcgac
     4321 gaccccgctc agcgccgaac gcctttcaca gtttcaggag tcgtcgcaga cgacgattga
     4381 gccgaatgtg tcggagcgcg tggaggcgct gccgccagtc gtcgcctcgc cgcccggctc
     4441 acagcgcgtg ggcacgatgg ctgcgcccac cgggtcgcgc gtgcccgtca ccgtctggat
     4501 cgcgggcgga gtgctcgccc tcctcttcgt cgtcgccggt ctttacttcg cactcaagcc
     4561 gacgcagagc gatgacgaga cgaacacgaa cctgcgggca aacgtaaacg ccacaccggc
     4621 cacgaaccgc ggcggcggcg gcaacgcgaa cgtcgcggcc acgcccgcgc ccggctccgc
     4681 gcccgacctc gtcgccctgc ccggcgggtc tttcatgatg ggacgcaacg atgtgccgcc
     4741 gcgcacgagc gaggtgtctg tggcctatca ggcgtgggtt tacaatcagt ggcccgcgca
     4801 ctccgtcaac gtctcagcct tcgccatcga ccggacggag gtgactaacg ccgagtacgc
     4861 cgagttcgtc gagaagacca atcacgcgcc gccggccgag tggggcggca ggcgagcgcc
     4921 ggcgggacag gagaagtggc ccgtcggcgg cgtctcgctc gacgacgccg aacggtttgc
     4981 gcgctggcgc tccgagcgtg acaaggtgca ataccgcgtg ccgaccgagg cggagtggga
     5041 gtttgcggcg cgcggcggcg cgggcgcggg ggcgcggctc tacccgtggg gcgcggagtg
     5101 ggccgacggg cgcgcgaatc tggagacgga cgcgctccgc cccgtcgggt cgttccccga
     5161 aggggcgacg ccgcagggcg cgctcgacat gatcggtaac gtctgggagt ggactgcgac
     5221 ccaggcgggg atgtacaggg gcaacaacct gctcgtactc gccagcggcg accgcggcaa
     5281 gttcgtcgtg cgcggcggct cctatcagag caagtcgcgc ggcgatgaac ccgtcacggc
     5341 cacggcccgc cgctgggtgc cgcgcgcgga acgcaacgcg ctcgtcggct tccggctcgt
     5401 gcgccccgga aagtgaccga ggcgcgatga acgaaagggc cgacacgtcc ccgcgtcagg
     5461 gcttctggac ttacacgcga ctcgcgctgg cggccctcgc cttcttcttg atcgtcatcg
     5521 ccgtctcgac cggactacgc gccccgcagg cgccggccac cgcgaaagac tctctgccgc
     5581 cgtccgtcct cggcacggag ctgcgcgctc tcgacggccc ggccttccgc ctctccgact
     5641 acaaagaccg tgtcgtcgtg ctcgacatct gggccacttg gtgcggcccc tgccggctgg
     5701 agattccaca cctcgtcagg ctgagtgagg agtatgggcc gcgcggcgtg gaggtgatcg
     5761 ggctgagcgt cgaagacccc gcgagcgcgg gcggggccgt gcgcgacttc gcgcgcgagt
     5821 tcgggatcaa ctaccgtctc ggctgggccg agcagcgttg gacgaacacc ctcacgcgcg
     5881 gcgccgccac catcccccag accttcgtca tcggtcgcgg cggacgccgg ctcctgcacc
     5941 tacagggcta caccccgcaa atctccgcca cactccgcga ggccatcgac cgcgcgctgg
     6001 ccgagtgaac ggcagtaccg cgagcggtag cgagcgggtg tcgctacggt cagaggcccg
     6061 ccccgcgcac gcgagacccg cgaggcttcc gctcgcggta ctgtgcgtgc cgctgacgcc
     6121 gccttgatta aaatcaatcc ttctcagcct ccttctcccg ctccttctct cgctcctcct
     6181 gaagctcgcg ctcgccctcg gccttaaggt cgcgcgcctc ctcggccagt tccgcgtccc
     6241 ccaagccctg cgcgacgcgc tccttcgcct tgccctcgat gcgcttcatc tcggcctcga
     6301 cgcggccctc gaccgcgtcg atatcggccg gctcgcccgt ctcctcctgc gtgacgatct
     6361 tgtccttgtc gatttccacg ttcgtcgcac ctcgcttccg tccgttaggt tgatttcgat
     6421 ttgcgtcatc ctcgcacccc cggagccgcg cggtcaaatg cgttgccacg cgcggcgcgc
     6481 ttggaatata cttcgcccgc ggtctcaaaa ccccgaagcc ccggccctct ccgccagaag
     6541 gcttatttga gccgcgcgaa cgtcgaacga gaggttcccc cgacatgact gaagacatta
     6601 cgggcttgct gcgccatggc cacgacacca tcgtgcgcag ttgggccgag aaggtgacgg
     6661 ccgacagacg cgtccgcacc gacgcccaac tgacctatct ccaactcctc gaccacgtcc
     6721 ccgacatcgt cgaagaactg cgcgaggccc tcgcttccgg cgagcgccca gatgggcaac
     6781 tgctcgaaga ggcccgcaac cacggccgca cgcgctggca gcagggctac gagctgaagg
     6841 aggtggtgcg cgaactcaca ctgctgcgcg ccgcggtcgt cgagtttatc gacgcctacc
     6901 gcggcgcgct ggccaccccg ccgcccgacc tcgtcaggtc ttacaaccgc atcgccggct
     6961 tcatggacga agagatttac cgcaccatcg aagcctatct cgaagcccac cgcgaggcca
     7021 tcgcgacggg cggcatcagc gttaactgac caataagcga cttatccaaa cgggcgtcgg
     7081 cgcgctttcc catgaagcgc gccgacgcct tttttgtcag cacaaaatat ttttccgatg
     7141 ggggcttgac tctggagccg gctccagggt ttacaatgtt ctcatgttga aaacggcgag
     7201 tttttcaggt gaggacagag ggcgctccgc cctgcgtatc ggcgaggtgg cgaaggcgtc
     7261 gggactcggg gttgagacgc tcaggttcta cgagcgacgc ggactcctcg gccgcccgcg
     7321 gcgcacggcc gccaactacc gcgtctacga cgactcggtt ctggagcgcc tcgccttcat
     7381 ccggcgcgcg caggccatcg gcttctcgct cgacgagata caaaccatca tcgccgagag
     7441 cgaggacggc cgccgcccct gcgggcacgt ccgcgagctg gcccggcgca agctctccga
     7501 actcgacgag cgcctgcgcg agctgcgcgc gcatcgagga gaactcgcgc gcctgctcgg
     7561 cgagtgggac gagcgcggcg aagaggccgg ccaattctgc ggcctcatcg agcacagccg
     7621 aatcgaagcg cgcaaagcac cggtcaggtc tttacgcgcc ggcaagaatg ggggacgtag
     7681 atgagcctga cggcggagaa agaactgacg cgctcgccgg agcgcatcga tctgccgatt
     7741 acggggatga cgtgcgcggc ctgcgcgcgg cgcatcgagc gcgcgctttc taaggcgcag
     7801 ggcgtcgcaa gcgccggggt caacttcgcc acggagcgcg caaccgtcga gttcgacgcg
     7861 gggcagacgg atgagggcgc gctgcgtcgc gtcgtcgagg agataggcta cggcgtccgc
     7921 gagttaccgc gtggccgggg cgcgggtgtt gaggaagacg cggaagagaa gtcgcgctct
     7981 gaagagcggc gcgagctgcg ccggcgtttc gtcgtcgccg cgctgctcac gctgcccgtc
     8041 gtcgtcatcg cgatgtcgca cggccggctg ccgctgacgg acggcccgtg ggtaaactac
     8101 ttacaactcg tgctcaccgg cacggtcgtc ttctacgccg gctggccctt cctgcgtggg
     8161 gcatggacgg cgctccgcca tcgcgccgcc gatatgaaca cgctcgtcgc gacgggcacg
     8221 ctcgcggcct tcctctactc ggtcgtcgcc accgtcgcgc cgcaactcgt caccacggcg
     8281 acacacgcgg gaatgccggc ggcgacgggc gagatgtcta tggggtcagg cactgcgccg
     8341 atggcgcccg tctatttcga ggcggcggcc gtcatcatca cgctcatcct gctcggccgc
     8401 ctgctcgaag cgcgggcgcg cgggcgcgcg ggcgaggcca tcaggaagct gctcggattg
     8461 caggcgcgca cggcacgcgt cgtccgcgac agcggcgcga cggaggatgt gccggttgaa
     8521 gcggtcgtcc ccggcgacgt gattttggtg cggccgggcg agaagattcc gaccgacggg
     8581 cgcgtcgtcg agggcgcttc ggccgtggac gagtcgatgc tgacgggcga gagcatgccg
     8641 gtcgagaagg ctgtgggcga ctcggtggtc ggggcgacgc ttaaccgcac ggggagtttc
     8701 aagttccggg cgacgcgcgt cgggagcgac acggtgttgc ggcagatcgt ccgcgtggtc
     8761 gaggaggcgc aggggcggcg cgcgcccgtg gcgcgtctgg ccgacaccgt gagcggctac
     8821 ttcacgcctg tcgtcatcgt gattgcgatt gcaaccttcg tcgtctggtt catcgccgcg
     8881 ccggtggaga cgcgcctcgc gacggcgctc gtcaacttcg tctcggtgct catcatcgcg
     8941 tgcccgtgcg cgctgggtct ggcgacgccg acagccgtga tggtcggcac ggggcgcggg
     9001 gccgcgctcg gcgtgctcat caagggcggc gaggcgctgg agacggcgca caaggttcag
     9061 accgtcgtcc tcgacaagac gggcacgctc acgaagggcg agccgggcgt gacggacgtg
     9121 gcggcggccg aaggcatcgc cgaagatgaa ctgctccggc tggccgccgc ggtcgagcgc
     9181 gcgagcgagc acccggtggg cgcggccgtc gtgcgccacg cggaggcgcg gggactggcg
     9241 acggcttcag cgtcggactt tggcgcgctc gccggccacg gcgtcgaggc ttcggtcgag
     9301 ggccggcgcg tgctcgtcgg caatctgaag ttgatgaacg agcgcggggt ctctctggga
     9361 agtttggaag cgcgcgccga tgagttcgcg gcggaaggga agacgccggt cttcgtggct
     9421 ctggacggac gcgcggcggg agtcgtggcg gtcgccgacc gactcaggcc cgagtcgcgg
     9481 gaggtggtcg cggcgctacg cgctttgggt ctcgaagtgt tgatgatcac gggcgacaac
     9541 cggcggacgg cccacacggt cgcgcgtgag ttgggcatcg agggcgtgct cgccgaggtg
     9601 ttgccggagg gcaaggccga ggaagttcgc aagctacagg ccgcgggccg ggtggtggcg
     9661 atggtcggcg acggcatcaa cgacgcgccg gcgctcgtac aggccgacct cggcatcgcc
     9721 gtcgggacgg gggcggacgt ggccatcgag gcgtcggacg tgacgctcgt ggggggcagc
     9781 ccgcgcggcg tgctgacggc catcgcgctc tcgcggcgga cgatgcgcgt catcaggcag
     9841 aacctcttct gggccttctt ctacaacgcg ctcggcattc cgctcgcggc cggattgctc
     9901 tacccgctca cgggctggct gctctcgccc gtctacgcga gcgcggcgat ggccttctct
     9961 tcggtctcgg tcgtcgccaa cagtttgcgg ctgcgacgct tcgggaaagg gcagtagccc
    10021 gaccgtgagg gagggcgcat gacgcgggag ctatacgccc tccctcacgg tcgggctact
    10081 gcctgttaga ctcagcatcc ccaaaggagt gatgtgatgg acacgcagga gatcatcgtg
    10141 ctggcgggcg gcgtcctgct cgccggctta gtcgtctggt tcttctttat gagcgagggc
    10201 gcgcgtgcgt cggccgaggt cgtcgagggc ggcgtccagc gcgtgcgggt gacggtcaag
    10261 ggcggctact cgccggacgt ggtggtcgtc aggaagggtg tgccggtcga actggacttc
    10321 taccgcgacg agacatcgtc gtgcaccgag gaggtcgtct tcggcgactt ccagattagc
    10381 cgtcgcctcc ccgccttcga gacgacgacc atcagactca cgcccgagcg cgccggcacg
    10441 ttcacgttca actgcggcat gaacatggtg cgtggcaagc tcgtcgtcga agactgacgg
    10501 cgcaaaggaa tatggcgcgt taggccgacg ttccggcgcg tgataaatta acagtgaata
    10561 aaatttccgc cacacgtcat tgagcggcga gtaaaaagtg ttgaggttta catgaaaatc
    10621 aaactgctct cattggccct ctttgcgctc gccgcgtctg cggcctgcgg cgcgggcgat
    10681 caacaacgcg cggtcaaaca ggtgaatgcg ccgccaaagg tcgaacaggt gaacgcgccg
    10741 ccgaaggtcg aagcggcgca cgcgcagaag aagcccgccg acgaccacgg gcacgaccac
    10801 ggcgagccgg gacgcgtccc cgccttcgag tcgaacccga agaacctgcc ggcgacgctc
    10861 gcgcccgagg cgtttaccgg caagacgcgt caggcctaca gggccgtgcg cgaaatcccg
    10921 gaaaccatcg cgcagttgcc ctgctactgt cactgcgacc gcggcttcgg ccacaagagt
    10981 ctgcacagtt gctacgtgga cggccacgcg tcgagctgcg ccgtctgcgt cgaagaagtt
    11041 ttgacggcct accgccttca gaaagaggag gggctgtcgc cggcgcagat acgcgagcgc
    11101 atcatcgcgg aatattccaa gctctaaggg gtcacagatg agaaaactat tcgcgaccgc
    11161 cgtcgccgct ctggcgcttt gcctgtgcgc ggccgacgcc gcatcagggc agggaacgaa
    11221 agggcaccgg gcgactcgga cgaaggacgc ttcgcgccac tcgaaggcgg cggccgacgc
    11281 cttcaactcc atcatgcgcg tccgcgacaa ggcgatcccg aaagaccttc tcgaaaacgc
    11341 ggaggccgtc gccgtcttcc ccggcgtgct caaggcggcc ttcatcatcg gcgggcgcaa
    11401 agggcagggc gtcatcagcc ggcgcacgcc gcagggctgg agcgcgcccg tcttctacaa
    11461 catgagcggc ggcagcatcg gcttccaggc gggcggctcc aagaccgact acgtgctgct
    11521 catcatgaac gaggacggat tgaagggact gctcgaagac aagttcgaga tcggcggcga
    11581 ggccggcgtg gcggccggcc ccgtcgggcg caccatctcg gcctcgacca cgccgacgct
    11641 cgacgccggc atcctctcct actcgcgcag taaaggcgtc ttcgccggcg cgtccatcaa
    11701 gggcgtcgcc atcacgcccg acaacgatct caaccgcgcc tactacggcc gcacggcgcg
    11761 cgaagtcctg acctctgggc agcgcccgcc gacgctcgtg cgctcgctgc cgctcgccat
    11821 cagccgctac tcgaacaggc gcgccgcccc cgcgccaaag aaatagtttt aagttcacac
    11881 ggaggagaag acttgatgaa ccgttcccgt cgattgagcg ccgcactgac agtctccctg
    11941 tttttgatcg tcgcgagttt cgtcgccccc gccggggcgc agaacgagag gccgcgaaag
    12001 gcggagacac cggcggcccg cccgagcgcg aagcagttga aggaggcgga gaagaaggcc
    12061 cgcgcggccg cgaaggtgtt cgacgagatc atggacgcgc ccgacagctc cgtgccgagg
    12121 gagttgctcg accgcgccga ggccgtcgcc gtcttccctg gcgtgctcaa ggccgggttg
    12181 ctcgtgggcg ggcgcggcgg cagcggcgtc atcagccgcc ggatagcggg cggctggagc
    12241 gcgccggcct acttcaagct gacgggcggc agtgtcggtt tgcagattgg ggcggcgaag
    12301 actgacctcg tgctgctgtt catgaacgag ccggcgctca aaggactcct tgaagacaag
    12361 ttcgagattg gcggcgaggc gagcgcagca gccggccccg tcggccgcgc cgccagcgcc
    12421 accacgaacg tcacgctcga cgccggcctc ctctcctact cgcgtagccg cggactcttc
    12481 gccggactcg aacttaaggg cgcgtggatc aaccccgaca acaacctgaa cgaagccctc
    12541 tacggcctca aggcccgcga tctcctcacc ggcgccacca aaatcaacat ggccgatgtc
    12601 cccgccggcg tcatcgtctt ccccaacgct ttggggcggt actcgatcaa gtaaaaggag
    12661 tcaggagtca gaagtcagga gtcagaataa aaacaggcgt cgcccgtcac actctcttca
    12721 ttctgactcc tgacttctga ctcctgactc ccggctttat tcttgacgcc ccccgcagtg
    12781 gcttctacaa tcacggccac gcggcagtta gagttcgttt gagtcgcaag tgttcgcgca
    12841 cgtttttgga gagccgaaga gatgcaaccc cgcacgcgca ggcagaagga agtcctcgac
    12901 tacatcacgc gcttcatcga gcgccacggc tatgagccgt cctacgcgca gatcgcgcgt
    12961 cacttccgcg tctcatcaaa agccaccgtc gccaagcaca tcacctcgct cgaacgtcag
    13021 ggcttgctca cgcgccggcg cgacgacggc ggctttggct taagcgtcca cgtcgaggaa
    13081 gtgccggccg acgcgacctg cgaggtgccg ctcatggggc gcgtggcggc gggcgctccg
    13141 ctcgacacgg tcgaagagcg cgagacgatt gtcatccccc gcttcctgct cgggcgcacg
    13201 cggcccggac aggtctacgc cctgcgcgtt cagggcgact cgatgatcga cgagcacatc
    13261 tgcgacggcg acatcgcctt agtcgagaat cgcaccgagg cccgcaacgg cgagatcgtc
    13321 gtcgctcttg tggacggcca gcgcgccacg ctcaaacgct tccaccgcgc cggtaacgac
    13381 gtggaactca agccggccaa ctccaacctc gaacccatca agctccacgc ctcgcgcgtc
    13441 tcagtccagg gcatcttccg cggcctcctc cgcccggcct cctaaagggg tcacgccccg
    13501 ccattcccaa gtagcgttag cgaacaaaaa cgctcactgc ctgtacgggt ttgtgagcgt
    13561 tttcgcgtcc tatttggcga ataacgcgct ccgccgcgct ggcataagtc ttgcccgact
    13621 cttcctgaaa cacgacgcaa ccggcgaaga ttttttaaga caccacgggc aatcgcccgc
    13681 ttaccgattc aaggagagtt aatcatgtcg gagagaacta cgaagaaggt cgccatcctc
    13741 atcgagcagc acgtcgagga cagcgaattc cagattcctt acaccgcgct ccagaaggcc
    13801 ggcgccgaag tgaccgtcct cggctcgcgc gtcaacgaaa cctacgcggg caagcaggcg
    13861 aaggtctcca tcaaggccga cgccaccaca acggacgccc gcgccgccga cttcgacgcc
    13921 gtcatcatcc ccggcggcat ggcccctgac aagatgcgta ccaacatgaa gaccgtgcgc
    13981 ttcgtgcgcg acgccgtcga gcagggcaag ctcgtcgccg ccgtctgcca cggcccacag
    14041 gtgctcatcg aggccgacgt gctccggggc aagcgcgcca cgggcttccg ctccatccgc
    14101 aaggacatgc aaaacgccgg cgccgacttc gagaacgaag cgctggtcgt tgacggccaa
    14161 ctgctcacct cgcgccgccc gtcggacttg cctgtcttca cggcggccat catccgtcgc
    14221 ctcggccttg agctgaagga cgtgagcctg ccggaagagg gcgtcgctta tgacgattgg
    14281 tggaagctcg cggaggagtg gggcggctcg acgaaggacg agatcgtgga cggtctcaac
    14341 acggccatcc gcggcgagcg ctacgctctg tcggccttcg agcaatacgt gttgaaggcg
    14401 accgcccccg acatcaagtc gctctttggc gagatcgtcg cctcgaagca gcgccacatc
    14461 cagagcctcg aagcgcggct cgcggccgtc ggcggcaagg aagacttgca ggcgggcggc
    14521 gcgggcgcgt gggcgaccct caagagctgg ctccagtcga gcgacgacgt gtcccttctg
    14581 cgccgcgcgc tcggcgacct ccagaccggg gtggttgacg ccttcaacct gaccaactcg
    14641 ttcaccgacc cggcgacagc cgccgtcttc gaccaaatcg agatcgacct cgccgagaac
    14701 gagcagcgcg tcgccgacct cctgcacgcg cgcacgagcg gacaggagtc gcagcccgcg
    14761 accccgtcca cgggcgcagc cgtcagcgcc tgagaagtag tatgaagaaa cagcgtgggg
    14821 cgaaagtgga agctcgaaag gcgtctccac tttcgcccca cgcttttctc tttcctcccg
    14881 cgcgcacgcc tcacgtaagt tcaatttgaa tgagacccgt cagcgaagcc cgtgcattct
    14941 gacgacactc ccgcgcgctg accgaaacgc acgggcgaag aaatcacccc gcacacgaac
    15001 tgtgaaaaca actctcggct gccccatgcg tccacccacg cgcgcgtcct aaacctttaa
    15061 atctcaacgt gaaagtcggc gccacgcggc ggccgacgtg cggcccgcac gcgccccaag
    15121 gcgccctctt tccccggcgc ggcgtggctg ctggcacgga gattgacaaa gggagcgcgg
    15181 aaagtctcgc gacgtttcgg gaaagacatt cattgattca tgcgtcggga cggaagaatc
    15241 attcgacaga tgggtgcaat ctacatgagc cgaatcgaca actctcgcac tgcggggcgg
    15301 acacttgcgt cgctcctgct gctcatttca ttaacctcaa gcctctgcgc cggcgtagcc
    15361 gcgggcgcac gaaagccgca gcgccggccg cgcatctccg cgccggcttc ctacttgtcg
    15421 gcgtcccacg tcgcgagtgc gctctcgtcg agcgcggaca cgatcagcga tccctatggc
    15481 aagctgacga cggatgtgca gaaggaggcc ggcaagaagg gctgggacgg gaatgacaag
    15541 acgcgcgtca tcgtccaact gagggacgcc gcgagcgcgc ggctcctcgc cctgctcgcg
    15601 tccggcgggg cctcggtgcg cgcacagcac cagtcgctcg aagtcctcat cctcgacgtg
    15661 ccggtgcggc tcctcccggc gctcacgcac ttccgcgagg ttgattcaat ctccacggac
    15721 agggagctga agccctcggg ccacgtcacg gcgaccacag ggcttgacga ggccgccgcc
    15781 gcctcctcct atgggaagct cgacgcggcg ggcgtcggca tcgccatcct cgactccggc
    15841 atcgacgagt cgcacccgtc cttcgccgtc aagggcgacg gccccagccg cgtcaggctc
    15901 agtctcgact tcacgggcga gggccgcacc gacgacccct acggccacgg cacgcacgtc
    15961 gccgcgctcg cgctcggcaa ccgcgcggtc ggcgaaggca agtacgccgg catcgcctcg
    16021 aacgccgaca tcctgaacct gcgcgtgctc gactccacgg gcgccggcac ggtctccagc
    16081 gtcctctccg cgctcgaatg gctctacgcc aaccgcacga aatacaacgt gcgcgtcgtc
    16141 aacctttccc tgggcgcgcc ggccgtcacg tcctaccggc tcgacccgct ctgccgcgcc
    16201 gcgcgccgcc tcgtcgcggc cggcatcgtg gtcgtcgccg ccgccggcaa cgagggcaaa
    16261 gaccgcgacg gcctcaaggt ctacggcgcc gtccactcgc cgggcatcga gccttcggtc
    16321 atcaccgtcg gcgccaccaa caccttcgac actgattcgc gcgccgacga cgccgtggcc
    16381 acctacagct cgcgcggccc cacgcgcggc tactggaccg acgcactcgg cgtgcgccac
    16441 tacgacaact tcgtcaagcc cgagatcgtc gcccccggca accgcctcgt cgcggcgaag
    16501 gctcgcgaca actacctcgt tcgcacatac ccgcacctcg acgccacgcg cgccggcggc
    16561 gccgcgatga tgtacatgag cggcacctcg atggccgccc cgctcgtcgc cggcgcggcc
    16621 gccgtgctcg tcaaggtcaa caacgctctg acgccgaacc tcgcgaaggc gattctgatg
    16681 tacacgtcgc aacctttggc cggctacaac cagttcgagc agggcgccgg acaactcaac
    16741 gtcgagggcg cggcgcgctt cgcgctgggg gtgcgcaagg acttcgcctc ccaactgctc
    16801 ggcgcgccgc tccagacgac gcacgaggtc gaagggggct tggtgaaggc cctgtcggac
    16861 ggcctgaacg gagcgtcagc cggcgccatc gagccgcgca ccaacatcag cggccacggc
    16921 ttcgcctggt cgcagacact cgtcatcaac cacgccgtcg tcttcggcgg cgagttgctg
    16981 acgcgctgcc agaacgttta cggcggcggc atcatgatga gcgacgccgt catcgagagc
    17041 ggcgtcacgc agtcgatcaa ccgcacggtg atgacgcgcg gcatcatgat gagcgaccag
    17101 atcgtgacga gcgacggcag cccgcagggc ggcggcctcc ccttcctgag caccggcatt
    17161 ctcatgggcg acggcatcat gatgagtgac ggcatcctca tgggcgacgg gattctgatg
    17221 ggtgatggaa tcctcatggg tgatggcatc ctcatgggcg acggcatcct catgggcgac
    17281 agccacctcg acggcgacga caccgccccc gtgcagtgaa gaaagtaaac gaacggacaa
    17341 aacgatcatg agtaacctac acacaattcg ccggggcgcc tctctgaccc tgaccctgat
    17401 tctgctcggc ggcgcgctcg ccggggggac gcgtctcgtc gtcccggcgg gcgcgcagtc
    17461 agggccgggc ggctcgtccg gctcttcggg gtcgtcgcag aagattaaag tgggcgccga
    17521 cctgcgcagg aaggcgaagg aggccccggc cttgcgcgtc aacgtcatcg tgcagcccgc
    17581 gggcgcgtgg actgtttcgc tcgactccga cctcaagggg aaggacgcgg ccgtcaggga
    17641 cgcccgccag aacttctccg cgcgctccgt cgagatgtac gcctccgagg tggagaacat
    17701 cgccaatcgc gacgacgtgg cctacgtctc gctcgaccgc gaggtgaaga agctcggcca
    17761 cgtttcgctc acgacgggcg ccgacgccgc gcgctcgatg ggcggctcga ccgtctacga
    17821 cggcgccggc atcggcatcg ccgtgctcga ctccggcatc gacccgaacc acgtcgcccc
    17881 caagaacgag agcctatcgg tgtcacgcgt cgccgcgagc gtggacttca cgggcgaggg
    17941 ccgcaccacc gacccctacg gccacggcac gcacgtcgcc tccatcgccg ccggcaacgg
    18001 caaggtcgcc tccgacgcct accgcggtat cgcgccggcc gcgcgcctcc tcaacctgcg
    18061 cgtgctcggc gccgacggcc gaggcacgac gagcagcctc ctcaacgcgc tcgactgggt
    18121 gatgacgaac cgcgcggccc acaacatccg cgtcgtcaat ctttctctgg gcaccgcggc
    18181 cgtcgattcc tacctcaacg acccgctgtg tctggccgtg cgccggctcg tggacgccgg
    18241 catcgtcgtc gtcgccgcgg ccggcaacga gggcaaggac gcctcgggga acaaaatcta
    18301 cgggcagatt cactcgcccg gcaacgaccc ttcagtgatt accgtcggcg cctcgaactc
    18361 cttcggcacc gacacgcgct cggacgacgc catgaccacc tacagctcgc gcggccccac
    18421 gcgcagcttc tggatcgacg cgcagggcgt caagcattac gacaacctca tcaagcccga
    18481 cctcgtcgcc cccggcaaca aaatcatctc ggcgcaggcg gccggtaatt acctagtgtc
    18541 gagcaacccc gtgctcgacg ccgacgtgag cggcaacgcc gcccgcgacc agatgtacct
    18601 gaacggcacg tcgatggcga cgcccgtcgt cgcgggcgcc gcggcccttc tactccaggc
    18661 taacccttcc ttgacgccca acctcgtcaa ggcgatcctg atgtacacgt cgcagcctct
    18721 ggccggctac aaccagttcg agcagggcgc cggacagatc aacatcgagg gcgcgatgcg
    18781 tctggcccgg ctcgtccgca ccgacctgac ctcggccacg cccgtcggca cgcccatgct
    18841 gacgggggcc gcgcccgccg cgcagaccac aatcgccggc tacaccttcc tgtgggcgca
    18901 gggcctcatc ctcgatcaca ccttcgtcac gggccagaat ctcatcacgc agtatcaggg
    18961 catctacggc acgggcgtga tgctctccga cgcgaccctg ctttcgagcg gggtgctgat
    19021 gggcgacggc gtgatgatga gcgacggcgt gatgatgagc gacaacatcc tgacgagcgc
    19081 cggcatcact ttcagcgaag gcacgatctt catccccacg aaccggctct actgcgacgg
    19141 cgtgctactc tcggacggcg tgttgatggg cgacggtgtt ctcatgggcg atggcgtgct
    19201 gatgggcgac ggcgtgctga tgggcgacca cgtgctgatg agtgacatcg cctcgcaggc
    19261 gggggcggcc atcatctcgg gcgaccccgg cccgttgatg ggcgtcgtca aagagacgct
    19321 gcccggcgca ccgctcagcc tcgcggtgtc ggccgtctcg tcgagtcagc tcaagctcac
    19381 ctggaaggac ggctcggcca acgaggacgg cttccagctc gagcgttgcg ccggctcgaa
    19441 ctgcaccaac ttcgcgcagg tggcgaccgt cgccgccaac acgattacct actcgaatag
    19501 cggcctcacc aaaaatacga cctaccgtta tcgcctccgc gcctacaacg gggccggcta
    19561 ctcgtcctac acggccatcg tcagcaaggc caccccgagg tagaccgggc gccgcggggg
    19621 cgccgccggg tgtcgttaac actcggccgc gccggccacc gcagcccaac cgaatctctc
    19681 ggcccccgcc cgttcagaag ttgaaccggg cgggggcgcc ctcgttcacc gccgcccggt
    19741 caggccatca aactgacgcg ccaaactgac gcgctcgacc cgccccgcca ctccaagggc
    19801 cgacacggcc ccgcataaac attctgaaat cgaccctgta aagattcgac gccggaggca
    19861 cgggcgttgc actacagttt tagacgcggc tcgcattgcg ggccgtgcgc ccctcgccca
    19921 gtcgaactct agctcatcgc tcccgacctc gcggaaggtg gcctgctaac ttgtacagac
    19981 cgcgtctttc aaaaccgttc atgtggctga cgattgccgc cggcgcggcc gtctgcctgc
    20041 acgcggcgcg cgaaattgcg gccggccaac tcgactaccg cttcctgctc ctcgccgccg
    20101 ccacgctcgg catcggctcc cgcatctcca tcaaaatccc ccacgacaag gggcagattt
    20161 ccgtctctga caccttcatc tttctcgcca tcctgctctt cgggccgggg ccggccgtgc
    20221 tgctcgccgc gaccgagtcg ttctgctctt cgctccgctt ctgcaagcat cgcgtcacgg
    20281 tcttcttcaa cgcggcggtg atggccgtct cgacgttttt gaccgtctat ctgctccagc
    20341 tttgtttcgg gtcggtcgcc gacctgcgcg gcggctactc ggccgtctac atcgtcggca
    20401 tctgcttcgt cgggttcgtg cagtacgtgc tcaactcggg gctgatcgcg ctcggctacg
    20461 cgctcaaaaa cgaccagcca ctctggacga cctggcacaa acacttcctc tggacttccg
    20521 tctcctactt cgcgggcgcc tcggccgccg gctttatcac gcgcctcatc gacgcctccg
    20581 gcttctacgc cctgctcgcc acggcgccca tcgtcgccat cgtttacatc acctacacca
    20641 cctacctgaa aaacgtcgaa gtcgcgcaag cgcaggccga gcaggccgag cggcacatgg
    20701 gcgcgctgcg cgaatccgag gaacgtttcc gcagcgtgtt cgatcaggcg gccggcatgg
    20761 cgctcgtctc ggccgagggg cgctgggtga gggtcaaccg ctccctgtgc gagatgctcg
    20821 gctacgaaga gcacgagttg ctcgctactg acttccaagc caccacgcac ccggacgacc
    20881 tcgggccgat gctcggcgaa gtggcccgcc tgctcgaagg ccgcatcgac cgcttccagt
    20941 ttgaacagcg attcctccac cgcagggggc aggagatgtg ggcgctcctg agcgtctcgc
    21001 tcgtgcatca cgcgcaggcc gccacgcacg agctgatctt ccagattcag gacatcaccg
    21061 accgcaagcg cgccgaggag cgactgatcc acgacgcctt ccacgacgtg ctcacgggcc
    21121 tcccgaaccg cgccctcttc atcgaccacc tcaagctctc cgtcgagcgc gccaagcgcc
    21181 gcgacaaacg cctcttcgcc gtcctcttcc tcgacctcga ccgcttcaag gtcatcaacg
    21241 actctctggg ccacatggtc ggcgaccagc tcctcatcgg catcgcgcgc cggttggaga
    21301 cctgcctgcg ccccggcgac acggtcgccc gcctcggcgg cgacgagttc accgtgctgc
    21361 tcgaagacct gacggggccg ggcgaggcca tcgaggtggc cgagcggctc cagaaggagc
    21421 tgtgcctgcc cttcaacctg aacggcaacg aggtcttcac caccgtcagc atcggcatcg
    21481 cgctctcgac taccggctac gagcgggccg aggaggttct gcgcgacgcc gacacggcca
    21541 tgtaccgcgc caagactttg ggcaaggccc gccacgaggt cttcgacaag gccatgcaca
    21601 cgcgggcgct gaacctgctt caggtggaga cggacttgcg ccgcgccgtc gagcgcgacg
    21661 agttcgtact ccactaccag cccatcgttt cggtcggcac gggcgaggtg cgcggcttcg
    21721 aggcgctcat cagatggcag caccccgagc gcggcttcgt ctcgccgatg gacttcatcc
    21781 ccatcgccga ggagacgggg atcatcgcgc agatcgggaa gtgggttttg gaggaggcgt
    21841 gccggcaggc gcgcgcgtgg cagaagaagt ttcccgccca cccgccgctt cagatgagcg
    21901 tcaacctctc gggcaagcag tttatgaagt ccgacctgat cggacaggtg cgcgccgcac
    21961 tcgccgccac gggcctcgac ccccgcactc tcaaactcga aattaccgag agcatcatga
    22021 tggagaatat cgagacggcg gccgagaccc tcggccaact gcgcgccctc ggcctccagc
    22081 tctccatcga cgacttcggc accggctact caagcctgag ctatctccac cgcttcccca
    22141 tctcgacgct caagatcgac cgctccttcg tcggccgcat ggacgccacg ggcgataacg
    22201 ccgagatcgt tcgcaccatc atcatgctgg cgcgcaacct cgacatggac gtggtggccg
    22261 aaggcgtcga gaccgagggg cagctcacac agctcaaggc gctcggctgc gagtacgcgc
    22321 aagggttcct cttctctcgt cccctcgacg cgcgcgccgc cgagtcgctc ctcgaagagc
    22381 atgagtcctc ccgccgcgcc gcgcccgcgc tggactggcc cgccgtcatc gtcacccgcg
    22441 acatgtagag tgctgcgcgc ccgccccgaa ggagcgtccg gcgcgggaac agggaagacg
    22501 cttcatgcac ggcgcgcttc tttaccgccg ccgccccccg cgtctaaact gctccacatg
    22561 gacgacacag ccttcgaggt taagccgagc cgcatccacg gccgaggact ctacgcgcgc
    22621 gcactcgtcc cggcccggcg gaagatgggc gagctgggcg gcgagcggat cacgaggcgc
    22681 gaggcccgcc ggcgcgcgcg tgaatccgac tgcatcacca tcgtcgaatt cgaggacggc
    22741 acggccctcg acgcctccag cggcggcaca cacttccgct acgtcaacca ctcctgcacg
    22801 cccaacacct tcatgcgctg catccgcggg cgcgtcgaat tctacgccct gcgtcagatc
    22861 aaccccggcg aggagctgac ctgcaactac ggtgagacgc accacgacgg cgccctcccc
    22921 tgccgctgcg gcagcgcccg atgcagaggg agcatctgaa cgggcaaaag ccggaaatcg
    22981 tgaatcgtca atcgtaaatc gtgaatcgat gaggttggcg ccccttcggc tcgagttaca
    23041 tagctcaagc cggccgccgt ctcaactacg attcacgatt cacgatttac gattcacggc
    23101 tttacgaagt ggctggttga ggtttgaggc gtaaaggaaa ttgccgtata actgcgtgac
    23161 agctatacgg caatttaaat tctggagcca ccttgcggac tcgaaccgca gacctgtcga
    23221 ttacgaatcg actgctctac caactgagct aaggtggctc gcgggtgaaa ttgaatatac
    23281 gaagcgtcgg cgcgcggtgt caagcgcgcc tctgacgagg aggaagagga gcgagatgcc
    23341 gaaagaccca acacgaaatg ttgaccgcta caaggtgcga ggcgggcagt tcgacgacct
    23401 cgacaggacg aagaacgacg gcgttatgac ggaagacgtg gcgcctttaa ccggcggccc
    23461 tgaggagacg aacttcattc cgggcgagcc ggcgcaggat gcggccgagc gcaagcagag
    23521 gctcatcgaa gaggtacacg cgaaggccga gcagcgccgg gaagcggaag ctgccaaggc
    23581 cagaaagtcc gcgcgcgcgg cccgcggcaa gaccgttgcg aagaagtctg cgaagaagtc
    23641 cgcaacgccg tcggtaaaga aggcggcaaa gaaatctgcg aagaa
//
LOCUS       DASVES010000509         4792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_13854, whole genome shotgun sequence.
ACCESSION   DASVES010000509 DASVES010000000
VERSION     DASVES010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13854"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VF240_21615"
     CDS             <1..1353
                     /locus_tag="VF240_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177872.1"
                     /translation="KNVSGLAANSPVSHVEPSRVALETAYVSFDRHMLDDLRPYVYKT
                     DDGGRTWQNITGNLPQNAYVWVVREDPKNPRLLYAGTELGLYVSYTGGGAWSFLGLKN
                     LPHVSVHDIFIHPRENDLILATHGRSVWILDDATPVQQLTPEIRRADAHLFDVRPALR
                     YTTRFTRYGIGDKVFTGPNPPYGALISYHFKTKLDDKVKPKIQILDGAGKVIADLENI
                     PKEQGLNRVSWNLRYGGPQVRRPPTDEETQFTGGPRGPQVLPGMYTAKLVVGDKSYTK
                     PVEVRLDPTISVLPADLKTQSDMTLRLRDMQSATNAALRTLDSLKSQIEFVERTVKDR
                     LTEVPKELSDRLTAHKKQVEELQNKLAQPEGGLGFSGRAQLIDRIGSLFFSIDGVNAA
                     PTHAQREHFGEVQTEFTQKIAEVNKFLADTVPQVNELLRRFNAPTLMTGKPIEPPAQP
                     "
     gene            complement(1586..1909)
                     /locus_tag="VF240_21620"
     CDS             complement(1586..1909)
                     /locus_tag="VF240_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177873.1"
                     /translation="MKRRTAALSAAIALGGLLTACGGDSNTNSNNANAARSNANATAG
                     NANGARRDGVIDTNANISANANSSSVPSNTAVVVNNNRNENTSGVSTMNGNRAGARNG
                     NTNRP"
     gene            complement(1951..2451)
                     /locus_tag="VF240_21625"
     CDS             complement(1951..2451)
                     /locus_tag="VF240_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177874.1"
                     /translation="MVTGLFKDRESAERAYGSLNSRGYSNDDFNMVMTDETRKKHFSD
                     DNDTELDTKAWEGAGKGAAIGGTVGGTLAALAAIGTTVALPGLGLLVAGPIAAALLGA
                     GTGGTIGGLIGGLVGSGIPEDRAKEYESGVKDGGILMGVNARSDEDAEYFETEFRNSR
                     GEKVYR"
     gene            complement(2761..2967)
                     /locus_tag="VF240_21630"
     CDS             complement(2761..2967)
                     /locus_tag="VF240_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177875.1"
                     /translation="MDDRLCWNCGHAFEHHLKIDGGCANESATGRCDCTGYEDAEYSG
                     HQRKRDLYTVSDRRRLSKRDAGAK"
     gene            3178..3981
                     /gene="arsM"
                     /locus_tag="VF240_21635"
     CDS             3178..3981
                     /gene="arsM"
                     /locus_tag="VF240_21635"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX7177876.1"
                     /translation="MRTPEDLKRAVREKYGALAVGDGAAGCGPCDGVSNIGEDYSALP
                     GYFPAADLGLGCGIPVEFARINEGETVIDLGSGAGNDAFVARTLVGERGRVIGVDMTE
                     AMVEKAQANARRLGFENVEFIVGEIEDLPVDSGAADIVISNCVLNLVPDKARAFAEVF
                     RVLRWGGRFSISDVVTAGKWEEAGKRAAEEYVGCVAGASDKDDYLATVRAAGFVNVAV
                     AKERRINIPKDVLRETAGAASARAEAINSDAVILSVTVYGEKLKQYRER"
     gene            4090..4710
                     /locus_tag="VF240_21640"
     CDS             4090..4710
                     /locus_tag="VF240_21640"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7177877.1"
                     /translation="MPEIENLGVFLVAAISLNMTPGPDTLYVLARSVGQGRRAGVVSV
                     LGGSTGRLIHTFATALGLSALLVSSPKAYAVVKLLGAVYLVYLGVRMLFGGGEKRERV
                     EAGGQSLARIYAQGLATNVLNPAVAAFFISFLPQFVNPERGSVAAQVVFLGVLFTTTA
                     TLWSLTVALLAGTFGNFLAAHPRFARAQRWLTGGILVALGARLAFV"
BASE COUNT          822 a         1537 c         1540 g          893 t
ORIGIN      
        1 aagaacgtgt cgggtcttgc ggccaactcg cccgtctcgc acgtcgagcc ttcgcgtgtc
       61 gcactggaga cggcctacgt ctccttcgac cgccacatgc tcgacgacct gcgcccctac
      121 gtctacaaga ccgacgacgg cgggcgcacg tggcagaaca tcacggggaa ccttccccag
      181 aacgcctacg tctgggtcgt gcgcgaagac ccgaagaacc cacgcttgct ctacgccggc
      241 acggaactcg gcctctacgt ctcctacacg ggcgggggcg cgtggtcgtt cctcggcctc
      301 aagaacctgc cgcacgtcag cgttcacgac atctttattc acccgcgcga gaacgatcta
      361 atactcgcca cgcacggccg cagcgtctgg attctcgacg acgccacgcc cgtccagcag
      421 ctcacgcccg aaatccgccg cgccgacgcg cacctcttcg acgtgcgccc ggccctgcgc
      481 tacaccacgc gcttcacacg ctacggcatc ggcgacaaag tcttcaccgg ccccaacccg
      541 ccctacggcg cgctcatcag ctaccacttc aagacgaagc tcgacgacaa ggtgaagccg
      601 aagattcaga ttctcgacgg cgcggggaag gtgattgcgg atttagagaa cattccgaag
      661 gagcagggcc tcaaccgcgt ctcgtggaat ttgcgctacg gcggcccgca ggtgcggcgg
      721 cctccgacgg acgaggagac gcagttcacg ggcggcccgc gcgggccgca ggtgctgccc
      781 ggcatgtaca cggccaaact cgtcgtcggc gacaagtctt atacgaaacc cgtcgaggtg
      841 cggctcgacc cgacgatctc cgtcctgccg gccgacctca agacgcagtc tgacatgacc
      901 ctgcgcctgc gcgacatgca gtcggcgacg aacgcggcgc tccgcacgct cgatagcctc
      961 aagagtcaga tcgagtttgt cgagcgcacg gtcaaagacc gtctgacgga agtgccgaag
     1021 gaactctccg accggctcac ggcgcacaag aagcaggtcg aggagttgca gaacaagctg
     1081 gcgcagcccg agggcggcct cggcttttcg ggccgcgcgc aactcatcga ccgcatcggc
     1141 agtctcttct tctccatcga cggcgtcaac gccgcgccca cgcacgccca gcgcgagcac
     1201 ttcggcgagg tgcagacgga gttcacacag aagatcgccg aggtcaacaa gttcctcgcc
     1261 gacaccgtcc cgcaagtcaa cgagttgctc cggcgtttca atgccccgac gctgatgaca
     1321 ggcaagccca tcgagccgcc ggcgcagccg taattcatca gtcatgtagg acaggcattc
     1381 ctgcctgtct tgagcgcgga gcgcgaacag gagattcgcc ggtctcaacg tcgagtgggc
     1441 gcggaacgtt tattcgcgct ccgcgctcaa gacaggcagg aatgcctgtc ctatggcaaa
     1501 tgagaattga aaagtgaagc ccccgcgagt ttggaaactc gcgggggctt ctaccgtcac
     1561 aggattgtct actgtcaggg tggcgttagg gccggttggt gttaccgttc cgtgcgccgg
     1621 ccctgttgcc gttcatggtc gagacgccgc tggtgttctc gttgcggttg ttgttgacaa
     1681 ccacagcggt gttcgacggc accgaactcg agttggcgtt ggccgagatg ttggcgttcg
     1741 tgtcgatcac cccatcacgc ctcgcgccgt tggcgttccc cgcggtcgca ttggcgttcg
     1801 agcgggccgc gttagcgttg ttcgagttcg tattcgagtc gccgccgcag gccgtgagca
     1861 ggccgccgag ggcaatcgcc gccgacaggg ctgctgttcg tcgcttcatc tgtttctcct
     1921 ccgtatcact gatcgtcgtg ccgacgactc ttatctgtag accttctcgc cgcggctgtt
     1981 ccgaaactcg gtctcgaagt actcggcgtc ctcgtccgag cgcgcattga cgcccatcag
     2041 gatgccgcca tccttcacgc cactctcata ttccttcgca cgatcctcgg ggatgccgga
     2101 gccgacgagg ccgccgatca aaccgccaat cgtgccgccg gtgccggcgc ccaacagtgc
     2161 cgccgcaatg gggccggcga cgaggaggcc gagaccgggc aacgccacgg tcgtgccgat
     2221 ggccgccagc gccgcgagcg tgccgccgac ggtgccgccg atggccgcgc ccttgccagc
     2281 gccttcccag gccttggtgt cgagttcggt gtcgttatcg tcggagaagt gcttcttgcg
     2341 cgtctcgtcc gtcatcacca tgttaaagtc gtcgttgctg tacccgcggc tgttcagtga
     2401 gccgtaggcg cgctcggcgc tctccctgtc cttgaagagt cccgtaacca tgcgcgaact
     2461 cgcttttgat gtacctgtag tttccgccat ttcttgagcc tcctagccta ttgtttgttc
     2521 gtaaattttt gccattgcac cctaagtcac acacatcagt gcatgatttt cataccaaaa
     2581 aaccgggtgc cattaggtta gaggcaatac ccttgccaca ggccgcgccg cggcgacaat
     2641 gctcatgttt atcgccctat gtgaggcaaa gggaacacgg atgacacgga tgaacacgga
     2701 tttgatccgt ccgcatccgt gtcatccgtg ttccctcagg aagggcgcgc gggccgcaag
     2761 ctatttcgcg ccggcgtctc tctttgacag gcggcggcgg tcggagaccg tgtagaggtc
     2821 tcgcttcctc tggtgcccgc tgtactcggc gtcttcgtag cccgtgcagt cgcagcgccc
     2881 cgtggcgctc tcgttcgcgc agccgccgtc gatcttgaga tggtgctcga aagcgtggcc
     2941 gcagttccaa caaagtctgt cgtccatcgg tcaggtctcc tttgtggtgc ggacgataac
     3001 gtaatggctt cccgcgcgga tttcaacagc cggcggacga aggacacggg attggcctcc
     3061 gcgccgtgac gaaaatggcg cttgcccacg cgcggattcg cgctacgatg cacgcggcaa
     3121 agacggacag acggcggcgc gcgccgtgcg aagcgttcgc ccagtggagg tgaagcgatg
     3181 agaacgccgg aagacttgaa gagggccgtc agggagaagt acggcgcgct ggccgtgggc
     3241 gacggcgcgg ccggctgcgg cccctgcgac ggcgtgtcga acattggcga agactactcc
     3301 gcgctccccg gctacttccc cgccgccgac ctcgggctgg gctgcggcat ccccgtcgaa
     3361 ttcgcgcgca tcaacgaggg cgagacggtg atcgatctcg gctccggcgc gggcaacgac
     3421 gccttcgtcg cgcgcacgct cgtcggagag cgcggccgcg tgattggtgt ggacatgacg
     3481 gaggcgatgg tcgagaaggc gcaggcgaac gcgcgccggc tcggcttcga gaatgtggag
     3541 ttcatcgtcg gggagattga agacttgccg gttgactcgg gcgcggccga catcgtcatc
     3601 agcaactgcg tgctcaatct cgtgcccgac aaggcgcggg cgttcgcgga agtcttccgc
     3661 gtcctcagat ggggcgggcg cttcagcatc tcggacgtgg tgacggccgg gaagtgggag
     3721 gaggcgggga agagagccgc cgaagagtac gtcggctgcg tggccggcgc gtctgacaaa
     3781 gatgactatc tggcgacggt gcgcgccgcg ggctttgtca acgtggcggt ggcgaaggag
     3841 cggcgcatca acattcccaa agatgtctta cgcgagacgg ccggggcggc atctgcgcgc
     3901 gccgaagcta tcaactcgga tgctgtaatc ttgagcgtga ccgtctacgg tgaaaagcta
     3961 aaacagtacc gcgagcggta gcgagcgggt gtgttggggc gcggcgaccc gctcgctacc
     4021 gctcgcggta ctgtctgcgc ttgccgtgct tgcgcggttg taaggcgccc acgctctcat
     4081 caacgatgaa tgcctgagat agaaaacctg ggcgtcttcc tcgtcgccgc catctctctg
     4141 aacatgacgc cggggccgga cacgctctac gtgctggcgc gcagcgtcgg gcagggccgg
     4201 cgtgccggcg tcgtctccgt gctcggcggg agcacgggcc ggctcatcca caccttcgcc
     4261 acggcgctcg gcctctcggc gctcctcgtc tcctcaccaa aagcctacgc ggtcgtcaag
     4321 ctcctcggcg ccgtctatct cgtctacctc ggcgtgcgaa tgctcttcgg cgggggtgaa
     4381 aaaagagaac gcgtggaagc cggcgggcag agcctcgcga ggatttacgc gcaggggttg
     4441 gcgacgaacg tcctgaaccc ggcggtggcg gcgttcttca tctcgttcct gccgcagttc
     4501 gttaatcctg agcgcgggtc ggtcgcggcg caggtggttt ttctcggcgt gctgttcacg
     4561 acgacggcga cgctgtggag cctgacggtc gcgctactcg ccggcacgtt tggcaacttc
     4621 ctcgccgcgc acccgcgctt cgcacgcgcg caacgttggc tgacgggcgg catccttgtg
     4681 gcgctcggcg cgcgactggc tttcgtgtga gtgtttggat gacaggtgag gtggcggaag
     4741 ctcgttgatt gagtgtggtg tgaggcagtt caccgcagag gcgcagagga cg
//
LOCUS       DASVES010000510        12286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_698380, whole genome shotgun sequence.
ACCESSION   DASVES010000510 DASVES010000000
VERSION     DASVES010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698380"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF240_21645"
     CDS             complement(<1..518)
                     /locus_tag="VF240_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7177878.1"
                     /translation="MKTGGRGDAEDTGLTDTSPRLLFAASFGRREGGWTLIELVIVIT
                     VLSILTVGVIPLARTAVRRQKEQRLREGLREMREAIKEFRRDTIGAPCCTQPGAAGQP
                     GAPPVPPPPPVPGQPVVPGQQQGQQLYLDPRSKVAISDRTIFDTSNLDHYPPTLKILV
                     EGVNVVPRLNLQG"
     gene            complement(542..3217)
                     /locus_tag="VF240_21650"
     CDS             complement(542..3217)
                     /locus_tag="VF240_21650"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX7177879.1"
                     /translation="MNTHARTRTLVALFLSALLLAAPMSAMAKKGEKNYKRGMTHERA
                     QQWEQAAQEFALAVAASPSDAEYQLHYRRAVFNASQVYMQKGRSLADKGDYVGAYNAF
                     RQAYGYDPVNELAANEMRRVLRLQRVKEGLDPEEPSGNGTNSSGGMRVQPSSLSTGGA
                     AARGQSVSAATPQIEERAQPLRQINYNGDLEDFIRKMAEELSLNVVFDQQFAQTKRNI
                     KIQLRDVTAAQALDYIFLAYGLFFQKLSRRTIIVADQSKRPQYQQLVLKTFYLYNIKP
                     TDARTLIQTALPANAGRQPQVVTNAETNSIIVRDTPENVRIIGELLKSVDKERAEVVM
                     EVAIYEVSRTDLLQVGNQLGTVDGLSNLGGLQTGSVLFDGARRIAGAAAGATLPLATG
                     VGLVIPASSLSLLQRKDNTRLVFSTAVHAFDDEKSEARVGARVPVQTASVYNGLTAPT
                     GTGGTPGGSAANVFGNGYPVIQYEDTGLVLDFTPKVYPNQEVQVKMNIETKDTDGNSL
                     TPTFTQRKITGTARIPNGRTMMLASIGQDRETNGRQGLPLLGLIPILGRLFTAPRKNN
                     IQSDVVITLTPRILRAPEINPSDEESKPSGTMQTPTSDTLEALVRDADRDDALAAARR
                     MPKNVTAQVNVPVEQPATSAPAQAAPVVQTAPTQTAPTQTASAAAAPNTPNTTAPQLP
                     AVIRGDDVSFVPAPKLLAESAASAPGKGDGATALVKEAFAKEAEPPAANISVVNTSAP
                     VQTPAPAAPVADAELILVPEQQELKVGERRRLMMMLKTDAPLGLVAATLRFDPRTVAV
                     RTVSGAVLSADKTGAPLVTHTIDPKGVLVVSVAPASGATPLAGEGLLLIIEVEGLAAG
                     EGTISIDADKVHFIATDGRSVRARAAVPARLKVVQ"
     gene            complement(3680..3886)
                     /locus_tag="VF240_21655"
     CDS             complement(3680..3886)
                     /locus_tag="VF240_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX7177880.1"
                     /translation="MNLTVEIEQEDDGRWIAEVVNLSGVMVYGGTRDEAVARVQALAL
                     RVLADRLEHGESAPDLMSVSFLAA"
     gene            complement(3915..5663)
                     /locus_tag="VF240_21660"
     CDS             complement(3915..5663)
                     /locus_tag="VF240_21660"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX7177881.1"
                     /translation="MSRVTEDIHAATTADDAPRQPSGGDTARLDRIDDSQQPPEVRAA
                     LNLARRYRLPYVDLLPPDGQSPIDYGLLGEVPVELMLRHMFVPLRREGKRLHIAMADP
                     SDLERLDEIASALRARVVPSVATAGAIEVVLRKGDATQRVLQEAASGFRISLVKETEH
                     GEEVLDLDRLATDSDMSPIIKLVDTVVYNAMESRASDIHIETRDNAVHVKYRIDGALY
                     EKVEPIDLAYHQTLISRIKVMSELDIAERRTPQDGRFRVRYKGRNVDFRVSIMPTIHG
                     EDAVIRILDKEQINEQFRDLKLDVVGFDADDLRNFRRYITEPYGMVLVTGPTGSGKTT
                     TLYAALNEIRNEEDKIVTIEDPVEYQLHGITQIPVNEKKGLTFARGLRSILRHDPDKI
                     MVGEIRDTETAQIAIQSALTGHLVFTTVHANNVIDVIGRFLNMGVEPYNFVSSLNCVL
                     AQRLVRILCSTCRRKYHPTDEELVESGLKAADHREGVFYMSVGCDACNHTGYRGRTAI
                     HELLNLSDNIREMIIDRRPGSEVRRAADKEGLRSLRESALKKVFSGVSTLHEINRVTF
                     VEELGSNGNHRDAPRS"
     gene            complement(5711..6940)
                     /locus_tag="VF240_21665"
     CDS             complement(5711..6940)
                     /locus_tag="VF240_21665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7177882.1"
                     /translation="MAEFICRLGTPAGEVVTRTIEAVGVGEARTRLESEGFRVFSVVP
                     PKTGGVAALTRGGKGGIHPRISANDFLLFNQQLASIIRAGIPILQAIMMLRKRSPAAR
                     MRMVLTDVEEKIRGGMALSSAFAAQGSIFPRIYTASVLAGERSGALDDVLSRYVSYLR
                     RGVEVRRKIRGALAYPLFLLVASFGMVAFLTVYVVPRMSGLFEGFGKQLPAITQFVIS
                     LSDFVSGNFYWLVPAIVGGAFGAFLWARTASGQLTIDRFLLKLPIVSQLLKQLAIAQV
                     TRSLSTLLAGGITLVESWEIAAEAITNKELRARSSAILPMVREGRSFTESLESAGWIP
                     PLALDMVGVGERSGSLREMLEEVAVFYDAESEVRLEQLTTVLEPAILLVMGGVVITIL
                     LAIYLPIIQSISNVTGR"
     gene            7426..8529
                     /locus_tag="VF240_21670"
     CDS             7426..8529
                     /locus_tag="VF240_21670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7177883.1"
                     /translation="MRDRRRRVFTSIALVSLIAAAVASNAPAAPIVPQTAPTQEQKAG
                     QTTAAPTPDTSNAKTTNKTATPASNPAADSRPASGPMVGVNQDDRHIVNADLITLTVT
                     VTDTYGRFVTGLGQKAFTILDDKTEQEIAYFSDEDAPVSLGVIFDVSGSMSGEKIKKA
                     REALARFVDTSHQGDEYFLIGFNSRAQLLLNKTRDSDAMLDKLTFVETKGMTALYDAA
                     YLGVEKVTRGTHEKKAVLLISDGQDNSSRYTFSELKRLLKETDVIIYSVGIVTDGDDG
                     TLGIGGRAILEELSGVSGGKAFFPSSAAELNDTFERIALELRTQYSIGYRPSNFANDG
                     RWHKLKVRVQPPRGMSRLFVRAKEGYFAVTNPK"
     gene            8886..10190
                     /locus_tag="VF240_21675"
     CDS             8886..10190
                     /locus_tag="VF240_21675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7177884.1"
                     /translation="MKQREVRTNATARFRPSLLILFALAAALLPAPSGAARPPQGAAD
                     GRPRRASTTANAQTQQTRPTNTPTPQPAKTSTPRPTPTPDDDDEPPPPRPRLQTSPGV
                     TATPTPDPNAAAGQEIGDDETITIDTRLVNLNVRVVDRDNRPIKDVRKEDFKVFDNNV
                     PQTVEFFETREVPISYGVVVDNSGSLRGQINQVIDASKTILESNREGDETFIVRFVSS
                     DEIKILQDWTANKQDLFDAVDDMFVEGGQTAVIDAVMLATEHASERRKGNDLEDKRRR
                     ALILVTDGEDRASFYKERDLFESMKERDVQIFVIGFVNELEREGGIIKKSKREKAVGL
                     LDRMAKETGGRAFYPTSLSDLPGIALEITKDMRTQFVLGYVPSNPAPPGEFRPVRVQV
                     ANAPGGEKRIAITRAGYTAQRGSSSGGAPATQRPNGRPTQKP"
     gene            complement(10279..10617)
                     /locus_tag="VF240_21680"
     CDS             complement(10279..10617)
                     /locus_tag="VF240_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177885.1"
                     /translation="MAVTENTQHKRRYKFGKRRGKKETAELSARLAPLEETAAADKMH
                     QAVPEGLPVNDDTIRVNTAALNLPERLVEEERTGARIFYLDPIVVAILVFMLAFIAFI
                     AWRITLMPAK"
     gene            complement(10608..11579)
                     /gene="corA"
                     /locus_tag="VF240_21685"
     CDS             complement(10608..11579)
                     /gene="corA"
                     /locus_tag="VF240_21685"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX7177886.1"
                     /translation="MITTTCLHSDTKKFDHDFPAAELSDFCENERNIVWADVSDPTSE
                     DFMELAQEFNFHPLSIEDCRNAHQRPKIEEFPGYYFIVLYEAELTGEHDDLELRELNI
                     FLGKNFLVTVHSRPIRAVETARRLWGEWTDRAEHGSGLLAYLTIDAIVDDYLPLLDII
                     SERMDDLEDRIFADFQQESIQDIFRFKKQLLYLRRSITPLRDVFNTMLRREQPIFARE
                     THVYFQDVFDHIIRVADTIDNLRDMLGSTMDAYLSVSGNRMNVVMKRLTSISTILMSA
                     TLIAGIYGMNFDFMPELKWRFGYVFALLWMMATALGLYFYLRKIKWL"
     gene            11725..>12286
                     /locus_tag="VF240_21690"
     CDS             11725..>12286
                     /locus_tag="VF240_21690"
                     /inference="COORDINATES: protein motif:HMM:NF021161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2391 family protein"
                     /protein_id="HEX7177887.1"
                     /translation="MQEYARGVAGGLLFSLPLLYTMEVWWAGFTAHPLRLAIYVAATF
                     VLLLGYNEFAGLRRDTSFVEVAIDSVEEMGLGLLIAAVMLWLLGRLTTEMPATEALGK
                     IVIEAMTVAIGVSVGTAQFGAVEDEDKGAGGGDESAGGTEGGDEVGAVRHFGGQVAVS
                     LCGAVLFAANLAPTEEIVQIAIEISAW"
BASE COUNT         2100 a         3934 c         4130 g         2122 t
ORIGIN      
        1 ccctgaaggt tgaggcgcgg gacgacgttg acgccctcga ccagaatttt gagcgtcggc
       61 gggtagtggt cgaggttgct ggtgtcgaag atcgtgcggt cgctgatggc caccttgctg
      121 cgggggtcaa ggtagagctg ctgtccctgc tgctggccgg gcacgacggg ctggccgggc
      181 accggcggcg gcgggggcac gggcggagcg ccgggctgcc ccgccgcgcc gggctgcgtg
      241 caacaggggg cgccgattgt gtcgcggcga aactccttga tggcctcgcg catctcgcgc
      301 aagccttcgc gcagccgctg ctccttctgg cggcggacgg ccgtgcgcgc cagcgggatg
      361 acgccgaccg tcaggatcga cagcacggtg atgacgatga cgagttcgat gagcgtccag
      421 ccgccctcgc ggcggccgaa ggaggcggcg aaaaggagac ggggagacgt gtccgtgagg
      481 ccagtgtcct ccgcgtctcc ccgtcccccc gtcttcatgt tacgaccctt agcgaacaac
      541 tttactgaac caccttcagg cgtgccggca cggcggccct ggcgcgcacg ctgcggccgt
      601 ccgtggcgat gaagtgtacc ttgtcggcgt cgatgctgat ggttccctcg cccgccgcga
      661 ggccctcgac ctcgatgatg agcagcagac cctcgcccgc caagggcgtt gcgcccgagg
      721 ccggcgcgac cgagacgacg agcacgccct tcgggtcgat ggtgtgggtg acgagcggcg
      781 ctcccgtctt gtcggccgag agcacggcgc cggagaccgt gcggacggcg acggtgcgtg
      841 gatcgaaccg gagcgtggcg gcgacgaggc cgagcggcgc gtccgtcttc aacatcatca
      901 tcagccggcg gcgctcgccc accttcaact cctgctgctc cggcacgagg attaactccg
      961 cgtccgccac cggcgcggcc ggagccggcg tctgcaccgg cgcactcgtg ttcactaccg
     1021 agatgttcgc cgcgggcggc tcagcctctt tcgcgaacgc ctccttgacg agggctgtcg
     1081 cgccgtcgcc cttgccgggg gccgaggcgg cgctttcggc gaggagtttc ggcgcgggta
     1141 cgaagctcac gtcgtcgccc ctgatcacgg ccggaagctg cggggcagtg gtgttggggg
     1201 tgttgggcgc ggcggcggcc gaggccgtct gcgtgggcgc cgtctgcgtg ggtgctgtct
     1261 ggacgactgg agccgcctgt gcgggcgcgc tcgtcgccgg ttgctcgacg ggcacgttga
     1321 cctgcgccgt gacgttcttg ggcatccggc gcgcggcggc gagcgcgtcg tcgcggtcgg
     1381 cgtcgcgcac caacgcttcg agcgtgtcgg aggtcggcgt ctgcatggtg ccgctgggct
     1441 tcgattcctc gtcgctcgga ttgatctcgg gggcgcgcag gatgcgcggc gtcagcgtga
     1501 tgaccacgtc ggactggatg ttgttcttgc gcggggcggt gaagagacgg ccgaggatgg
     1561 ggattaagcc gaggagcggc agtccctgcc ggccgttggt ctcgcgatcc tgaccgatgc
     1621 tggcgagcat catcgtgcgg ccgttcggga tacgcgccgt gcccgtgatc ttgcgctgcg
     1681 tgaaggtcgg cgtcaggctg ttgccgtcgg tgtccttcgt ctcgatgttc atcttcacct
     1741 ggacttcctg attcggatag accttcgggg tgaagtccag caccaggccg gtgtcttcat
     1801 actggatgac ggggtagccg ttgccgaaga cgttggcggc ggagccgccg ggggtcccgc
     1861 ccgtccccgt gggcgccgtc aggccgttgt agacggaggc cgtctgcacg gggacgcgcg
     1921 cgccgactct ggcctccgac ttctcgtcgt cgaaggcgtg caccgccgtg gagaagacca
     1981 gacgcgtgtt gtccttgcgc tggaggagcg agagcgaaga ggccgggatg acgaggccga
     2041 cgccggtcgc cagcgggagc gtcgcgccgg cggcggcccc ggcgatgcgg cgcgcgccgt
     2101 cgaagaggac ggagccggtc tggaggccgc cgaggttcga gagcccatcc accgtgccga
     2161 gctggttgcc gacctgcaac aggtcggtgc gcgaaacctc gtagatggcc acctccatga
     2221 cgacctcggc gcgctcctta tcgacgctct tcaaaagctc gccgatgatg cggacgttct
     2281 cgggcgtgtc gcggacgatg atcgagttgg tctcggcgtt ggtgacgacc tgcggctggc
     2341 gaccggcgtt ggccggcagc gccgtctgga tgagcgtgcg cgcgtcggtc ggcttgatgt
     2401 tgtagaggta gaaggtcttc aaaaccaact gctggtactg cgggcgcttc gactggtcgg
     2461 cgacgatgat cgtgcgccgc gagagcttct ggaagaagag gccgtaggcg aggaagatgt
     2521 agtcgagcgc ctgcgcggcc gtcacgtcgc ggagttggat tttgatgttg cgcttggtct
     2581 gggcgaactg ctggtcgaag acgacgttga ggctcagctc ctcggccatc ttgcggatga
     2641 agtcttcgag gtcgccgttg tagttgatct gccggagcgg ctgcgcgcgc tcttcgatct
     2701 gcggcgtcgc ggcggagacc gactggccgc gcgccgccgc gccgccggtg gaaagcgaag
     2761 acggctggac gcgcatgccg ccgctgctgt tagtgccgtt gccggacggc tcctcggggt
     2821 cgaggccctc cttcacgcgc tggaggcgga gcacgcggcg catctcgttg gcggccagct
     2881 cgttgacggg gtcgtagccg taagcctgcc ggaaggcgtt gtaggccccg acgtagtcgc
     2941 ccttgtcggc gagcgagcgg cccttctgca tgtagacctg cgaggcgttg aagacggcgc
     3001 ggcggtagtg gagctggtac tcggcgtccg acggcgaggc ggccacggcg agggcgaact
     3061 cctgcgcggc ctgctcccac tgctgagcgc gctcatgcgt catgccgcgc ttgtagttct
     3121 tctcgccctt cttggccatc gccgacatcg gcgcggccag taggagcgcg gacaaaaaga
     3181 gcgccacgag cgtgcgcgtg cgtgcgtgtg tgttcatctc gaaaaacctc acttcaactc
     3241 ctcgcgggga gaacgtaaaa cctcggctgc ttttgatggg cgggagacgc cgcgcgcgac
     3301 gcctcatgac tctcggtggc ttaacggatt aggacgaagc ctgaagaact gggagcgacc
     3361 ctgcgcgtcc gcggccggtt cgtcccctcg cggtcaactt acacgccggg gcgctaataa
     3421 tagttcctta aacgcggcgg acacaaggcg aaaaaagcat caaccgtcta caaatcttcc
     3481 ggtttcagtc cggtctgctt tgctatacga acaagcattt taggcccaat ctcttcttta
     3541 tcgtggaaag aaaagacgaa gtctgcccaa cccacgcggg agaggacacg atgagagctg
     3601 gcagtttgtc gcttgacgcc ccagccgatg cgcaaaagtg cggccaacac gcgccgggct
     3661 ttcgctgatt accactgcgt catgcggcaa ggaaagagac gctcatcaag tccggcgcac
     3721 tctctccgtg ctccaacctg tcggcgagga cgcgcaaagc cagcgcctga acacgcgcga
     3781 cggcctcatc gcgggttccc ccgtagacca tcactcccga gaggttgacg acctcggcaa
     3841 tccagcgccc gtcgtcttcc tgttcgatct ctacggtcag gttcatctca cctcacaccg
     3901 aaaagccggc cccattaact ccgcggcgcg tcgcggtggt tgccgttcga gccgagttcc
     3961 tcgacgaagg tcacgcggtt gatctcgtgc agcgtcgaga cgccgctgaa gaccttcttg
     4021 agagccgact cgcgcaggga gcgcaggccc tccttgtcgg cggcgcggcg cacctccgag
     4081 ccgggccggc ggtcgatgat catctcgcgg atgttgtccg acagattcaa aagctcgtgg
     4141 atggcggtgc ggccacggta gcccgtgtgg ttgcaggcgt cgcagccgac gctcatgtag
     4201 aacacgccct cgcggtgatc cgccgccttg agacccgact ccaccaactc ctcgtccgtc
     4261 gggtggtact tgcgccggca ggtcgagcag agtatacgca ccaggcgctg ggcgagcacg
     4321 cagttcagag acgagacaaa gttgtacggc tcgacgccca tgttgaggaa gcggccgatg
     4381 acatcgatga cgttgttggc gtggacggtc gtaaagacga ggtggccggt caaggccgac
     4441 tggatggcga tctgggccgt ctcggtgtcg cggatttcgc cgaccatgat cttgtccggg
     4501 tcgtggcgca ggatggagcg caggccgcgc gcaaacgtca gccccttctt ttcattgacg
     4561 ggaatctgcg tgatgccgtg gagctgatac tccacggggt cttcgatggt gacgatcttg
     4621 tcttcctcgt tgcgaatctc gttgagggcc gcgtacagag tagtcgtctt acccgaaccc
     4681 gtcggccccg tgacgagcac catgccgtaa ggctcggtga tgtagcggcg gaagttgcgg
     4741 aggtcgtcgg cgtcgaaacc cactacgtcg agcttgaggt cgcggaactg ctcgttgatc
     4801 tgctccttgt cgaggatacg aatcaccgcg tcctcgccgt ggatggtcgg catgatggag
     4861 acgcggaagt ccacgttgcg gcccttgtag cggacgcgga agcggccgtc ctgcggggtg
     4921 cggcgctcgg cgatgtcgag ttccgacatc accttgatgc gcgagatcaa agtctggtga
     4981 taggcgaggt caatcggctc gaccttctcg tagagcgcgc cgtcgatgcg gtacttgacg
     5041 tggacggcgt tgtcgcgggt ctcgatgtgg atgtcggagg cgcgcgactc catggcgttg
     5101 tagacgaccg tatcgaccag cttgatgatg ggcgacatgt cgctgtcggt cgccagccgg
     5161 tcgaggtcta agacctcctc gccgtgctcg gtctccttga cgagcgagat gcgaaagccg
     5221 gaggcggctt cttgcaggac gcgctgcgtg gcgtcacctt ttcgcaggac gacctcgatg
     5281 gccccggccg tcgccaccga tgggacgacg cgcgcccgca gggcgctggc gatctcgtcc
     5341 aagcgctcca aatcggacgg gtcggccatc gcgatgtgca ggcgcttgcc ctcgcgcctc
     5401 aagggcacga acatgtgccg cagcatcagc tcgaccggca cctcgccgag cagaccgtag
     5461 tcgatgggcg actgaccgtc gggcggcagt agatcgacgt aaggcaggcg gtagcggcgg
     5521 gccagattga gtgcggcgcg cacctcgggc ggctgctggg agtcgtcgat gcggtccagg
     5581 cgcgccgtgt cgccgccgct cggctgccgg ggcgcgtcgt cggccgtcgt ggcggcgtgg
     5641 atgtcttcgg taactcggct cattaaggtt gttctgatgc ttccgagggc gtggcggtgg
     5701 ctcgccggtt tcagcggccc gtgacgttgg aaatggactg gatgatcggc aggtagatcg
     5761 ccagcaggat cgtgatgacg accccgccca tcaccagcag aatcgcaggt tcgagcacgg
     5821 tcgtcaactg ctccagccgc acctccgact ccgcgtcgta gaagaccgcc acctcttcga
     5881 gcatctcgcg caaactgccc gaacgctcgc ccacgccgac catgtcgagc gccagcggag
     5941 gtatccaccc ggccgactcc agactctccg taaacgagcg cccctcgcgc accatcggca
     6001 ggatggccga cgagcgcgcc cgcagctcct tgttcgtgat cgcctcggcg gcaatctccc
     6061 acgactcgac gagcgtgatg ccgcccgcca gaagggtcga gagcgagcgc gtcacctgcg
     6121 cgatggcgag ctgcttgagg agttggctga cgatgggcag cttgagcagg aagcggtcga
     6181 tggttagttg ccccgaggcc gtgcgcgccc acaggaaggc gccgaaggcg ccgccgacga
     6241 ttgccggcac gagccagtag aagttgccgg agacgaaatc cgagaggctg atgacgaact
     6301 gcgtgatggc cggcagttgc ttgccgaagc cctcgaagag gcccgacatg cgcggcacga
     6361 cgtagacggt caagaatgcc accatgccga acgaggcgac gagcaggaaa agcggatagg
     6421 ccagcgcccc gcgaatcttg cgacgcacct cgacgccgcg ccgcaggtag gagacgtagc
     6481 gcgagagcac gtcgtcgagc gcgccggagc gttcgcccgc cagcaccgac gccgtgtaga
     6541 tgcgcgggaa gatcgacccc tgcgcggcga aggccgagga gagcgccatg ccgccgcgaa
     6601 tcttctcctc cacatccgtg agcaccatcc gcatgcgcgc cgcgggcgag cgcttcctga
     6661 gcatcatgat ggcctgaagg atggggatgc cggcgcggat gatggaggcg agttgctggt
     6721 tgaagagcag gaagtcgttg gcggagatgc gcgggtggat gccgcctttg cccccacgcg
     6781 tgagcgccgc cacacccccg gtcttcggcg gcactacgga gaagacccga aagccctcgg
     6841 attcgagccg cgtccgcgct tcgcccacgc cgacggcctc gattgtgcgc gtcaccacct
     6901 cgcccgcggg cgttcccaaa cgacagatga attcggccat gcttactgag ttagaacgat
     6961 taagcggatt tactttcgcc ggctgaacgg ggggccggct gcttcggaac aggccggcaa
     7021 gtataacacg cggccgcgcc gggcctccca cacgccccgc ccggccgcgc gtcctcttcc
     7081 ctcccgcacc tacacgctcg ccacgctttg aagttccgcc gagcgcggtc acaaatgtgg
     7141 acggaagttc aggacaccga acgacaccga accttgacac ttactcaaca ggcaaagtct
     7201 tgccgaggga gcacagcctg tcctacaatc gcctcgaaac ggacagctaa ggagtctccc
     7261 cggccccgct ttttttaaag cctcgtgaaa caaccgaaag gtcttcggcc gccatcctag
     7321 cggcaaccgc agaccaacgc ttgtgggccg cgccccggcg cgaaggttct acacgattcc
     7381 ccacttcggc caggcccgga gcgaccatat caggagggtt tacctatgag agatcggcgc
     7441 cggagagtct tcactagcat cgcgctcgtc tccttgatcg ccgccgccgt cgcctcaaac
     7501 gcgcccgccg cccccatcgt cccgcagacg gcccccacgc aggaacagaa agccggacag
     7561 accaccgccg ccccgacgcc cgacacctcc aacgcgaaga caacgaacaa gacggccacg
     7621 cccgcgtcga accccgcggc cgactcgcgc ccggcctccg gccccatggt cggcgtcaat
     7681 caggacgacc gccacatcgt caacgccgac ctcatcacgc tgaccgtgac ggtgacggac
     7741 acctacggcc gcttcgtcac gggcctcggc cagaaggcct ttaccattct cgacgacaag
     7801 accgagcagg agatcgccta cttctcggac gaggacgcgc ccgtctctct gggcgtcatc
     7861 ttcgacgtgt caggctcgat gagcggcgag aagatcaaga aggcgcgcga ggccctcgcc
     7921 cgcttcgtag acaccagtca tcagggcgac gaatatttcc tcatcggctt caactcccgc
     7981 gcccagctcc tcctgaacaa gacgcgcgac tccgacgcca tgctcgataa gctgaccttc
     8041 gtcgagacga aggggatgac ggcgctctac gacgccgcct acctcggcgt cgagaaggtg
     8101 acgcgcggca cgcacgagaa gaaggcggtg ctgctcatca gcgacggtca ggacaacagt
     8161 tcccgctaca ccttctccga gctgaagcgg ctgctcaagg agactgatgt catcatctac
     8221 tccgtcggga tcgtcaccga cggcgacgac ggcacgctcg gcataggggg ccgcgccatc
     8281 ctcgaagaac tgtccggcgt ctccggcggc aaggcgttct tcccatcgtc cgccgccgaa
     8341 ctgaacgaca ccttcgagcg catcgcgctg gagctgcgga cgcaatactc catcggctac
     8401 cgcccctcga acttcgccaa cgacggccgc tggcacaagc tcaaggtcag ggttcagccc
     8461 ccgcgcggca tgtcgcgtct cttcgtccgc gccaaagagg gctacttcgc cgtcacgaat
     8521 ccgaaataag aggtcagaga ttagaggttg gaggtcagtg aaagacggat gagtttttac
     8581 taacctccaa cctctgacct ctatatcttt ggcaactgcg ggcggcccga gggcatcttt
     8641 tgaggtgcgc gcaagccgaa cgcgcggtgg tcgcgtccga ccgaagtttt tgtactttgc
     8701 cccggcaacg ccgaagcctt cggataaggt caggcaatac gggcgagcgc ccgtcgagag
     8761 gctcgctccc ggtcggcggg agacggggtc gcccggcggg cgcttactaa cgatgggggc
     8821 ttcgcttccg gggcggcggc ggggctatac tgctgaaacg gcccccagcg gaggtgttca
     8881 cagacatgaa gcaacgagag gttcgcacga acgcgacggc acgttttcgg ccatcgctcc
     8941 tgattctctt cgcgctggcc gcggcactcc tccccgcgcc gtccggcgct gcgcgtccgc
     9001 cgcagggcgc ggccgacgga cggccccggc gcgcctccac caccgccaac gcgcagactc
     9061 agcaaacgcg gcccaccaac acccccaccc cgcagcccgc gaagacttcc acgccccgcc
     9121 cgacgccgac gcccgacgac gacgacgagc cgccgccgcc gcgccccaga cttcagactt
     9181 cgcccggcgt cacggccacg ccgacgcccg accccaacgc cgcggccggt caggagatcg
     9241 gcgacgacga gaccatcacc atcgacacgc gtctcgtcaa cctcaacgtc cgcgtcgttg
     9301 accgcgacaa ccgccccatc aaggacgtgc gtaaggaaga cttcaaagtc ttcgacaaca
     9361 acgtgcccca gacggtcgag ttcttcgaga cgcgcgaggt gcccatcagc tacggcgtcg
     9421 tggtggacaa ctccggctcc ctgcgcggcc agatcaatca ggtcatcgac gccagcaaga
     9481 ccattctgga gagcaaccgc gagggcgacg agacgttcat cgtccgcttc gtctcctccg
     9541 acgagatcaa gattttgcag gactggacgg ccaacaagca ggacttgttc gacgccgtcg
     9601 atgacatgtt cgtcgagggc gggcagacgg ccgtcatcga cgccgtgatg ctcgccaccg
     9661 aacacgcctc cgagcgccgc aagggcaacg acctcgaaga caagcgccgc cgcgccctca
     9721 tcctcgtcac cgacggcgaa gaccgcgcca gcttctataa ggagcgcgac ctcttcgagt
     9781 cgatgaagga gcgggacgtg cagattttcg tcatcggctt cgtcaacgag ctggagcgcg
     9841 agggcggcat catcaagaag tcgaagcgcg agaaggccgt cggcctgctc gaccgcatgg
     9901 ccaaggagac aggcgggcgc gccttctatc caacctcgct ctcggattta cccggcatcg
     9961 ccctcgaaat cacgaaggac atgcggacgc agttcgtgct cggctacgtc ccgtcgaacc
    10021 cggcgccccc cggcgagttc cgccccgtgc gcgtccaggt ggccaacgct ccgggaggcg
    10081 agaagcgcat cgccatcacc cgcgccggct acaccgccca gcgcggcagc agcagcggtg
    10141 gcgcccccgc cacacagcgg cccaacgggc gcccgacgca gaaaccttag cggagcgggc
    10201 agggggtaaa ggggaagggg taagggagaa gacacatgct tctcccttac cccttcccct
    10261 ttaccccctg cccccttttt atttcgccgg catgagcgtg atgcgccagg cgatgaaggc
    10321 gatgaaggcg agcatgaaga cgaggatggc gacgacgatg gggtcgagat agaagatgcg
    10381 cgcgccggtg cgttcctcct cgacgagtcg ctccggaagg ttgagcgcgg cggtgttgac
    10441 gcggatggtg tcgtcgttga cgggcagccc ttcgggcacg gcctgatgca tcttgtcggc
    10501 cgcggccgtc tcttcgagcg gggcgagacg cgctgaaagc tcggccgtct ctttcttccc
    10561 gcgccgcttg ccaaacttat agcgccgttt gtgctgagtg ttctccgtca cagccattta
    10621 atcttcctca ggtagaagta tagcccgagc gccgtcgcca tcatccacag cagggcgaag
    10681 acgtagccga agcgccattt cagctccggc atgaagtcga agttcatgcc gtagatgccg
    10741 gcgatgagcg tggccgacat caggatggtc gagatcgagg tcagccgctt catcaccacg
    10801 ttcatgcggt tgccggagac ggacaggtag gcgtccatcg tcgagccgag catgtcgcgc
    10861 aggttgtcga tggtgtcggc gacgcggatg atgtggtcga acacgtcctg aaagtagacg
    10921 tgtgtctcgc gcgcgaagat gggctgctcg cgccggagca tggtgttgaa aacgtcgcgc
    10981 aggggggtga tggagcggcg cagatagagg agctgcttct tgaagcggaa gatgtcttga
    11041 atcgactcct gctggaagtc ggcgaagatg cggtcttcga ggtcgtccat gcgctcgctg
    11101 atgatgtcta agagcggcag gtagtcgtcc acgatggcgt ctatcgtgag gtaggccaga
    11161 aggcccgagc cgtgctcggc gcggtcggtc cactcgcccc acaggcggcg ggccgtctcg
    11221 acggcgcgga tgggccggct gtggacggtg acgaggaaat ttttgccgag gaagatgttc
    11281 agctcgcgca gttcgaggtc gtcgtgctcg cccgtgagtt ccgcttcgta gaggacgatg
    11341 aagtagtagc cggggaactc ttcgatcttc gggcgctgat gcgcgttgcg acagtcttcg
    11401 atggagagcg ggtggaagtt gaactcctga gccagctcca tgaagtcttc gctcgtcggg
    11461 tcggacacgt cggcccagac gatgttccgc tcgttctcgc agaagtcgct gagttcggcc
    11521 gccgggaagt cgtggtcgaa cttcttcgtg tcgctgtgca ggcaagtggt cgtaatcacg
    11581 actcgtcgtt cgtcctcctt cgctcgcgtg ctctttcgag tatacttgct ccgcgcgaat
    11641 tttggacggg taaaatcgcg gggccgacgg cgcgccggct ctcgcgccag tcgggagact
    11701 ctattgcgga cgcttagacg ctcagtgcaa gagtacgcgc gcggcgtggc gggcgggctg
    11761 ctgttcagcc tgccgctgct ctacacgatg gaggtgtggt gggccggctt caccgcgcac
    11821 cctttgcgtc tcgccatcta cgtcgccgcc accttcgttc ttcttctggg ctacaacgag
    11881 ttcgcgggtc tgcggcgcga caccagcttc gtggaggtcg ccattgactc ggtcgaggag
    11941 atgggcctcg gtctgttgat cgcggccgtc atgctgtggc tgctggggcg cctgacgacg
    12001 gagatgcccg cgaccgaggc tttggggaag atcgtcatcg aggcgatgac ggtcgccatc
    12061 ggcgtctcgg tcggcacggc gcagttcggc gcggtcgagg acgaggacaa gggcgcgggc
    12121 ggcggcgacg aatctgcggg cgggactgag ggcggcgacg aggtgggagc cgtgcgccac
    12181 ttcggcgggc aggtcgccgt ctcgctgtgc ggcgcggtgc tgttcgcggc caacctcgcg
    12241 ccgaccgagg agatcgtcca gatcgccatc gaaatatcgg cgtggc
//
LOCUS       DASVES010000511        18022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1086300, whole genome shotgun sequence.
ACCESSION   DASVES010000511 DASVES010000000
VERSION     DASVES010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086300"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF240_21695"
     CDS             <1..618
                     /locus_tag="VF240_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177888.1"
                     /translation="RQMPLRGLAADRKGTVYVVQRGDIQMFEGPTGKPLGRIGYPEGS
                     SFDDVVATPDGGLVTSWHRHRDDIVRLDSSGKVTRVIRSAISGQTDRSELNTRVTADG
                     AGNIFALGSFNNAVFKFSPEGRFLTQFGSSGDQTGQFRAPYAITADNQGRVYVADIKG
                     VQVFAPDGRYLDFFPVEGPASGMVFNDKNEIFVVARTQVFKLAVK"
     gene            775..1848
                     /gene="tsaD"
                     /locus_tag="VF240_21700"
     CDS             775..1848
                     /gene="tsaD"
                     /locus_tag="VF240_21700"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7177889.1"
                     /translation="MLVLGIESSCDETAAAIVRDGCEILSSVISSQIDIHKQYGGVVP
                     ELASREHLDKIVPVVEEAFERAGVGNDEIDGIAVTAGPGLVGSLLVGVSYAKAMAYAL
                     SKPIVGVNHIEGHVYSVVFENPPIEYPAFALIVSGGHTNLFLVPEPGRYKVLARTRDD
                     AAGEAFDKVAKMLGLGYPGGPIIEKLAREGDPRAVRFSIPRMGDGRPDFSFSGLKTAV
                     SKHVRESGLQSVAPGAEPSQAVKDLAASFQSVVVRSLVGTTERLAREHRPRTLIVAGG
                     VACNMALREAAGEAGARLGLPVYFPSRHLSTDNAAMIAAAGAVKLRAGERAGLDLNAD
                     VSMRLQNVDVEDAAMRGRVRYRL"
     gene            complement(2065..2421)
                     /locus_tag="VF240_21705"
     CDS             complement(2065..2421)
                     /locus_tag="VF240_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177890.1"
                     /translation="MRQKNLAAVVAAMLMIFSTTALAADTNGQDTNSRLVNAKVVEVT
                     DGHISVITPKGIEHVIAIDDDGTRVMIDEEFVSLKDLREGDVVTVELDTLNPLKFAMS
                     IEISKQAASQVARARP"
     gene            complement(2730..3848)
                     /gene="dnaJ"
                     /locus_tag="VF240_21710"
     CDS             complement(2730..3848)
                     /gene="dnaJ"
                     /locus_tag="VF240_21710"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7177891.1"
                     /translation="MSKRDYYEVLGVARTATEQEIKSAYRRLAVKFHPDKNPGNAGAE
                     EKFKEGAEAYAVLSDSEQRARYDRFGHAGVSSSAGVNWGGAAGFGGIEDILGDLFGDV
                     FGGGRAGSSSRRTSAQRGADLRYDLQMTLEDAAQGMTAQLRIPRLEACDACEGTGAAK
                     GTQPENCATCGGAGQVRFQQGFFSVARTCGNCRGAGRIIRNPCDACQGAGRVEREKQL
                     EVKIPAGVETGSRLRITGEGEAGTQGGPSGDLYVVIRVKEHDLFERQGNNLYSSVPIS
                     FAQAALGSETTVQTLDAQQQLKIPAGTQTGTVFRVKGAGMPVLGGRGRGDLFVAVTVV
                     TPKTLSRDQRRLLEQLAEVEDHDLEDKGLMDKVRDIFG"
     gene            complement(3964..5817)
                     /gene="dnaK"
                     /locus_tag="VF240_21715"
     CDS             complement(3964..5817)
                     /gene="dnaK"
                     /locus_tag="VF240_21715"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7177892.1"
                     /translation="MGKVVGIDLGTTNCCVAVLEGGAVQIVPNKEGGRTTPTVVGFTD
                     KGERLVGQIAKRQAVTNAANTVYAVKRLIGRKHGDPEVARAKETVPFEIVDSTNGDAR
                     IQVQGRVYSPQEISGIVLQRLKAAAEDFLGEAVTEAIVTVPAYFDDTQRQATKDAGRI
                     AGLKIERIINEPTAAALAYGMGRTENERIAVYDLGGGTFDISILEINNGVFEVLSTSG
                     NTFLGGEDFDERIIGWMTKQFQEETGIDLRSDRLALQRLKEAAERAKCELSSLTETNM
                     NLPFIAADASGPKHFSKTLTREAFEGLVADLVEQTIEPCQKALWDAKLQPGDIEKVLL
                     VGGQTRSPIIARTVREIFGKEPSAEINPDEVVAMGAAIQGGVLAGDVKDIVLLDVLPL
                     SLGVETRGGLFTKIIERNSTIPLRSSQIFTTVVDNQPSVEIHVLQGEREVALGNRSLG
                     KFELVGIPPGPRGGPQIEVTFEVDANGILSVSAQDKTSGRKQQMKVTPTSGLSTDEID
                     RLITEATRSVEADQEIKELISMRNRLDSLVRNTQRTYREFGNSLTPEERQAAQTLLID
                     AEQASKTASPDELKRLQEEVDQLAKKLTLAMFNSAGSDGSAGEPDDHNARL"
     gene            complement(5883..6761)
                     /gene="grpE"
                     /locus_tag="VF240_21720"
     CDS             complement(5883..6761)
                     /gene="grpE"
                     /locus_tag="VF240_21720"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7177893.1"
                     /translation="MSSMMNKDRQNPSRIPVRFVDEEGKEGEPRDESGADDLTPEEIG
                     RESSYEGETEVQQRIDRGAEGGDDADTAGGPDPADLPERREEQDTNPSHREGDAEARN
                     AAAASDAADTTARHAGGEGGPVLAELIATRAELKRVESELEKIRAEHADLLDRITRRQ
                     ADFDNYRKRIERERAETYNRAAGDVVGQLLPVIDNLRRALDAEATVQAGESEEFRHFL
                     QGVELINRQLGGVLEALGVKPVLTVGQPFDPHVHEAVAAESSDRHEPDTVIEELTRGY
                     RLGDKLIRPAMVKVAK"
     gene            complement(6826..7938)
                     /gene="hrcA"
                     /locus_tag="VF240_21725"
     CDS             complement(6826..7938)
                     /gene="hrcA"
                     /locus_tag="VF240_21725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7177894.1"
                     /translation="MAIRGSTKQAEWRPGARGQAVLSAIIREHLRTGEPVGSKTIADG
                     FAGGAGWSSATIRNVMAELEEAGLVEQPHTSAGRVPTDTGYRFYVDHLASGTRLSRAD
                     MAAIDRTLGLTNLENGASPQRLMERVSHLLSELSENVGIVVSPPLADNRLQHIEFLRL
                     ADGRVLAVLVFASGVVQDKLIRVDEPLGQDELERAARYLNTEFSGKSLQSIRAEVLAL
                     MREEKALYDELLQNVALLCEKSLDGGESDAGEVYLDGASNILAKPDFSDIERLRELFQ
                     TFEAKSRLVKILNECIAREPVFGDVHVVIGREHSAPSLRACALISAPYRVGGGDAAGS
                     VGVVGPMRMEYARTMAVVSYVAHIFERTLRDEAAII"
     gene            8510..9241
                     /locus_tag="VF240_21730"
     CDS             8510..9241
                     /locus_tag="VF240_21730"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEX7177895.1"
                     /translation="MQDSQPLLHTLFLDAFRQLAPSRPAPDVDVRYYPYAGLNHTIRL
                     RTGRVYVRVSDIFRGAPDNVHRALAFILVAKLLRRRTPQLHERTYRNYACQPEVLRAS
                     DLARRERGRKQISSARGSRYDLEKMFARLNRRYFDGELPPPVLTWSRRRTRTILGHHD
                     GVHETIVISKTLDSADVPEWFVEFILYHEMLHIKHPARLINGRRFYHTKAFRSEEQRF
                     PFYSEAQEWLDRIARQRRSLRARAA"
     gene            9602..9841
                     /locus_tag="VF240_21735"
     CDS             9602..9841
                     /locus_tag="VF240_21735"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEX7177896.1"
                     /translation="MSDKVFKSVEIVGTSEQSFSHAIEVAVERARQTLRELSWFVVGE
                     MRGGLQQGRLEYQVTLRVFFKLDAAGGQGPDGDLA"
     gene            9985..10548
                     /gene="efp"
                     /locus_tag="VF240_21740"
     CDS             9985..10548
                     /gene="efp"
                     /locus_tag="VF240_21740"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX7177897.1"
                     /translation="MAFPATQIRRGMVILFEGAPCKVLDARHHTPGNLRAMMQTKLRN
                     LRTGSSFEHRFRSADTVERAHLEQHEMEYMYSDGAHHHFMNTENYEQTALSGEELGDM
                     AQWLTAGLRIEVEFYDGAPIGITLPPSLELAVVETEPTMKGATVSNVNKPAKLENGVT
                     ILVPPFVNEGDRIRVDPIEGRYMERAK"
     gene            10637..12025
                     /locus_tag="VF240_21745"
     CDS             10637..12025
                     /locus_tag="VF240_21745"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX7177898.1"
                     /translation="MYLFYSILLTFGFALLLPRFLFDALRHGKYAAGFRQRMGSLPSL
                     SAGGAQKPVVWLHCVSVGETQAARPLARAVLEHFPAHALVVSTTTLTGQRVAREAFAG
                     MAAEVFYFPFDWAWSVRRALRRVRPSAVLVMETELWPRFFRECRKADVRLAVVNGRIS
                     ENSFRRYRLVRPFISRVLNDLDLAVMQSESDAERARLLGLVPDKVRVSGNIKFDFEID
                     AAEQTLTADLRRRFGFDDEGRPLIVAASTHAPEERIVLDAFRELRGAGAGRGPRLLVA
                     PRHPERFAEVAALLDASGFAWARRSATPLPTDAQADVVLLDTVGELRAAYPLAELVFV
                     GGSIAQVGGHNVMEPAASARCVVTGAHTSNFRAVVGAFLERDALLQLPPLAESEAAHV
                     LAETFRNLLEDADRRRRVGQNARAVLDENRGATGRTIALLAPIFEARRESAGLRSARG
                     RGLASDLRPPLP"
     gene            12022..13077
                     /gene="lpxK"
                     /locus_tag="VF240_21750"
     CDS             12022..13077
                     /gene="lpxK"
                     /locus_tag="VF240_21750"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX7177899.1"
                     /translation="MNTSFALAPLSALYGAAVRARLALYRGGALRQERAGAPVISVGN
                     ITAGGTGKTPLVEWVARAVAREGRRACVLTRGYGRRDERLRVVVSDGERLLADAPEAG
                     DEPRMLAEQLLGVASVVSDADRAAAARWALENLRAQAFVMDDGFQHLRLARDLDVVAL
                     DATAPWGGGHLLPRGRLREPRRGIGRADCVVITRADLAGDAAARALREEVSGLCGGAA
                     LVVEARARTTGLRRLADDTAGDEFVSAADVRSVGAFCAVGNPQSFFEHLRRDGHEPVL
                     ARAFTDHHAYTQSDLDSLTRAATEAGAHSLLTTAKDAVKLRRLSTKLPIYVVEIALEI
                     DEEEKLLELVRGALAKS"
     gene            complement(13123..14847)
                     /locus_tag="VF240_21755"
     CDS             complement(13123..14847)
                     /locus_tag="VF240_21755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7177900.1"
                     /translation="MKKCPKCDKEFEDANTLCPSDGSVLKRQDELVGQTLAGKYRIEE
                     KLSEGGMGCVYRATHVLMEKTLAVKVLHPALAADDNIVARFTREARAASRISHPHAIT
                     VTDFGEAENGVVFLVMEYLRGRTLKEVIRSEGPMPLKRAAEIVRQMAGALEAAHNEGV
                     VHRDLKSDNIMLDETPGGDWAKVLDFGIAKIKETAENQDPELTAPNLIIGTPQYMSPE
                     QCSGASKIDARSDIYSFGIIVYEMLAGHVPFTGDSPTEVMMMQMQEAAPSLHTERDDL
                     PASVDAVVQRALAKNPDHRFQNAGELSGAFASAASADAPTEAAAAANAAARASATDGR
                     GVPETSRIVVPTGENEAPRTTGAVASDEEEATVVSARTTRTREETREEYAPLPPPPAA
                     SFNPWRIAIPALIVLAAVFGLFYLFQGNDTAGETQPPLASDPNSQPVVSVTPPTGAGE
                     SNITPSTGLTTPTPGEVIAGETIPASGNANANLGVAPTPAGGDAGRNENANTEGEEEE
                     AEPTPEPTPPERNTNRRAPTPPPPVNANPDPDEAPPPRKPAPTPPPPVSGDVPAPAGT
                     SGVPPGRL"
     gene            complement(15160..15474)
                     /locus_tag="VF240_21760"
     CDS             complement(15160..15474)
                     /locus_tag="VF240_21760"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX7177901.1"
                     /translation="MNKLRMAFFAALGVLASAAPALAQAGAADNDASINKYKALAAGF
                     GFALAAALAAIGPSRVAAAAAEGVARNPGAGARIQTLMILGLVFIETLVLFTLVVVFA
                     KL"
     gene            complement(15848..16789)
                     /gene="atpB"
                     /locus_tag="VF240_21765"
     CDS             complement(15848..16789)
                     /gene="atpB"
                     /locus_tag="VF240_21765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7177902.1"
                     /translation="MFFLFSDPAPAAQGAAADHAAPAAAAHHTPAIVEFINHQIGKPV
                     HEFQLHYTKPLWDRFFSNFGTTAENVFGKYTEENAVPWYTVMFILACLISLALVWLLR
                     GRMSQDEPGNGQQMLETGVQSIRGLLLDNVGPSGMAYLPVVATFAVLILVSNLMGLFP
                     GLMSPTASTSVTFALGISSFVYYNYVGIKENGVGGHLKHFAGPIPWLAILMFPIELIS
                     NLVRPMSLGVRLFGNLFGDEQILGTIASLVPWWLPVPVLIMPLSVFVAFMQTFIFTLL
                     SILYIAEVSHHEGDEHHGADDQHGHTVDEGGYATAHP"
     gene            complement(16793..17236)
                     /locus_tag="VF240_21770"
     CDS             complement(16793..17236)
                     /locus_tag="VF240_21770"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX7177903.1"
                     /translation="MSVIAGDAHSNSPAAGGGEEPAAVERRIFRGMCAAVAVAVLLSA
                     ALLPWRTTTGLLTGGLLSLLNFHWLRTSVAAMFGSTAPGVRATWRLSRYVLRYVVVAG
                     VATAAYALDLVSLVAVLAGLCSSAAAVLIEGFMQLYFAVIRREET"
     gene            complement(17294..17533)
                     /locus_tag="VF240_21775"
     CDS             complement(17294..17533)
                     /locus_tag="VF240_21775"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX7177904.1"
                     /translation="MADDSQNEESRDEAAVTRRSSTAYAAGLAIFFSVASFLGLGWLL
                     DSYFGTGPWLIITGILLGSVAGFYQFIRIMSRNNN"
     gene            complement(17567..17827)
                     /locus_tag="VF240_21780"
     CDS             complement(17567..17827)
                     /locus_tag="VF240_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177905.1"
                     /translation="MDKFKIVLALLVLLLAGIGAFFVFGLIAWAFQYLLWIGVLAVIV
                     GVAYKVLKRPARPQLNAWDAPDWELDRADRVLEDMKNKQLLK"
BASE COUNT         2943 a         6049 c         5767 g         3263 t
ORIGIN      
        1 cgacagatgc cgctgcgcgg actcgcggcc gaccgcaagg gcaccgtcta cgtcgtacag
       61 cgcggcgaca tacagatgtt cgaggggccg acgggcaagc cgctcgggcg catcggctac
      121 cccgagggaa gcagcttcga cgacgtggtg gcgacgcccg acggcggcct cgtcacctcg
      181 tggcaccgtc accgcgacga catcgtgcgc ctcgactcct cgggcaaggt cacgcgggtc
      241 atccgctcgg ccatcagcgg acagaccgac cgctcggagc tgaacacgag agtcaccgcc
      301 gatggcgccg gcaacatctt cgcgctcggc agcttcaaca acgcggtctt caagttctcg
      361 cccgagggtc gtttcctgac gcagttcggc agctccggcg accagacggg gcagtttcgc
      421 gcgccctacg ccatcacggc cgacaatcag gggcgcgtct acgtcgccga catcaagggc
      481 gtgcaggtgt tcgcccccga cggacgctac ctcgacttct tccccgtcga aggccccgcc
      541 tccggcatgg tcttcaacga caagaacgaa atcttcgtcg tcgcccgcac gcaggtcttc
      601 aagctcgcgg tgaaatagac cacagtaccg cgagcggtag cgagcggggc gcgtgcgggc
      661 aaggtcggct tcatcgtagg ctcgcccgct cgctaccgct cgcggtactg ttccagcctc
      721 atgtcgcgct tttgtgaccg tcctgcctgc cctgctatca tcacctcaca aacaatgctc
      781 gtactcggca tcgaaagctc ctgcgacgaa acggccgccg ccatcgtgcg cgacggttgc
      841 gaaatactgt cctccgtcat ctcctcgcag atcgacattc acaagcaata cggcggcgtc
      901 gttcccgaac tcgcctcgcg cgagcatctc gacaagatcg tgcccgtcgt tgaggaggcg
      961 tttgagcgcg ccggtgttgg caatgatgag attgatggga tcgccgtcac cgccggcccc
     1021 ggtctcgtcg gctcgctgct cgtcggcgtc tcctacgcga aggcgatggc ctacgcgctc
     1081 tcgaagccca tcgtcggcgt caaccacatc gaggggcacg tctattcggt cgtcttcgag
     1141 aacccgccca tcgaataccc cgccttcgcg ctcatcgtct cgggcggaca cacgaacctc
     1201 ttcctcgtgc cggagccggg ccgctacaag gttctggcgc gcacgcgcga cgacgcggcg
     1261 ggtgaggcgt tcgacaaggt ggcgaagatg ctcggactgg gctatccggg cggccccatc
     1321 atcgagaaat tggcacgtga gggcgacccg cgcgccgtgc gcttcagcat cccgcgcatg
     1381 ggcgacggcc gacccgactt cagcttcagc ggactgaaga cggccgtgtc aaaacatgtg
     1441 cgcgagtcgg ggctgcaaag cgtggcgccg ggcgcggagc cgtcgcaggc ggttaaagat
     1501 ttggcggcaa gtttccagag cgtcgttgtc cgctcgctgg tgggcacgac cgagcggctg
     1561 gcccgtgagc accggccgcg cacgctgatc gtcgcgggcg gcgtcgcctg caacatggcg
     1621 ttgcgcgagg cggcgggcga ggccggcgcg cgtctcggtc tccccgtcta cttcccctcc
     1681 cggcacctct cgaccgacaa cgccgccatg atcgccgccg ccggcgccgt caaactccgg
     1741 gcgggcgaac gcgccggcct cgacctcaac gccgacgtgt cgatgcgtct tcagaatgtt
     1801 gacgtggaag acgcggcgat gaggggccgc gtgaggtaca gactataaag ggagtcagga
     1861 gacagaagtc aggagtcaga ataaagataa agaacctgct tttattctga ctcctgactt
     1921 ctgtctcctg actccctgcc tttagaaacg gaaaccgccc cgccttctag cgagggaggc
     1981 ggagcggctt cgttttgccg gtgcgcgagt acgacgctca agaagacgtc ttgcaccggc
     2041 gaacatgtgc gaggtgcggc tggctcaggg tctggctcgc gccacctgac tcgccgcctg
     2101 cttggaaatc tcgatactca tggcgaactt gagcgggttc aaagtgtcca gctcgaccgt
     2161 caccacgtcc ccctctcgca ggtcttttag agaaacaaat tcttcgtcga tcatcaccct
     2221 cgtgccgtcg tcgtcgatgg cgatgacgtg ctcgataccc tttggcgtga tgaccgagat
     2281 gtggccatcc gtcacttcga cgacctttgc gttgacgaga cgcgaattag tgtcctggcc
     2341 attcgtatcg gcggcgagcg ccgtggtcga gaagatcatc agcatggccg ccacaaccgc
     2401 cgcgaggttt ttttgtctca tcaacattat caatcccctt accgtgttat gaggggcgcg
     2461 aaagttttgc gccctgcctc aagttcttgg caatggtcat gccgcaactg cggcccgccc
     2521 ggaaattcgt gcttaaagcc tgcgttgtga agattaagac taacccgaag cgcgtgcgac
     2581 gcggtcagcg tcgcgggcgc gcgcgtatga tgtgaggacg acttgtatcg atacaaaact
     2641 gccgcgcccg ctgcgttatg ggaagagaaa ggggccgcct cgaatcttca aggcggcccc
     2701 ttatgcttgg gagcgtcggc tgtttcgtgt cagccaaaga tgtcgcggac tttgtccatc
     2761 agtcccttgt cttcaaggtc gtggtcttcg acctcggcga gctgttcaag caggcggcgc
     2821 tgatcccgcg agagcgtctt gggcgtgacg accgtgacgg cgacgaagag gtcgccgcgg
     2881 ccccggccgc cgagcaccgg catccccgcg cccttgacgc ggaagaccgt gccggtctgc
     2941 gtgccggcgg gtattttcaa ttgctgctgg gcgtcgagcg tctgcacggt ggtctcggag
     3001 ccgagagcgg cctgcgcgaa cgagatgggg acggacgagt agagattgtt gccctgccgc
     3061 tcgaagaggt cgtgctcctt gacgcggatg acgacgtaga ggtcgcccga cggcccgccc
     3121 tgcgtccccg cttcgccctc gcccgtgatt cgcagccgcg accccgtttc gacgccggcc
     3181 ggaattttta cttcgagctg cttctcgcgc tcgacgcgtc ccgcgccctg acaggcgtcg
     3241 caggggttgc ggatgatccg gcccgcgccg cggcagttgc cgcacgtgcg cgccaccgag
     3301 aagaagccct gctggaaacg cacctggccc gcgccgccgc acgtcgcgca attctcgggc
     3361 tgtgtgccct tggccgcgcc cgtcccttcg cacgcgtcgc aggcttcgag gcgcgggatg
     3421 cggagttgcg ccgtcatccc ctgcgccgcg tcttcgagcg tcatctgcaa gtcgtagcgg
     3481 aggtcggcgc ccctctgcgc cgacgtgcgt cgggaggagg agccggcgcg gcctccgccg
     3541 aacacgtcgc cgaacaggtc gccgaggatg tcctcaatgc cgccgaagcc ggcggcgccg
     3601 ccccagttga cgcccgcgct gctcgacacg ccggcgtgtc cgaagcggtc gtagcgcgcg
     3661 cgctgctcgg agtcggagag cacggcgtaa gcctcggcgc cctccttgaa cttctcctcc
     3721 gctccggcgt tgcccggatt cttgtccggg tggaacttca ccgccaaacg ccggtacgcg
     3781 ctcttgatct cctgctcggt cgccgtgcgg gcgacgccta agacttcgta gtaatctctt
     3841 ttactcatta gctcgtaaat tgaaaggccg taaattgaaa atcgtaaatc gaaaatcgtg
     3901 aactagatta tacaagcatc gattcacgat tgacgattca cgattgacgg tttttatggt
     3961 tcttcacagt ctcgcgttgt ggtcgtccgg ctccccggcc gagccatcgc tgccggcgga
     4021 gttgaacatg gccagcgtca gtttcttggc gagttgatcg acctcttcct ggaggcgctt
     4081 aagctcgtcg gggcttgcgg tctttgacgc ctgctcggcg tcgatcagga gggtctgggc
     4141 cgcctgccgc tcctcgggcg tgagcgagtt gccgaactcg cggtaggtgc gctgggtgtt
     4201 gcgcaccaga ctgtcgagcc ggttacgcat gctgatcagt tccttgatct cctgatcggc
     4261 ctcgacagaa cgcgtcgcct cggtgatcag cctgtcgatc tcgtccgtgg agagacccga
     4321 agtgggcgtg accttcatct gctgcttgcg tccgctcgtc ttgtcctgcg ccgagacgct
     4381 gaggatgccg ttggcatcga cctcgaaggt gacctcgatc tgcggccccc cgcgcggccc
     4441 cggagggatg ccgaccagct cgaacttgcc caagctacgg ttgccgagcg ccacctcgcg
     4501 ctcgccctga aggacgtgga tttcgaccga cggctggttg tcaacgacgg tggtgaaaat
     4561 ctggctggag cgcaaaggga tggtcgagtt gcgttcgatg atcttggtga agaggccgcc
     4621 gcgcgtctcg acgcccaggg agagcgggag cacgtcgagg aggacgatgt ctttaacgtc
     4681 gccggcgagc acgccgccct gaatggccgc gcccatcgcc acgacctcgt cggggttgat
     4741 ctcggccgac ggctccttgc cgaaaatctc gcgcaccgtg cgggcgataa tcggggagcg
     4801 tgtctgcccg ccgacgagca agaccttctc gatgtcgccg ggctggagct tcgcgtccca
     4861 gagcgccttc tggcacggct cgatggtctg ctcgacgagg tcggcgacga ggccctcgaa
     4921 cgcttcgcgc gtcagcgtct tactgaagtg cttcggcccc gaagcgtcgg ccgcgatgaa
     4981 cggcaggttc atgttcgtct cggtcaggga agacagctcg cacttggcgc gctcggcggc
     5041 ctccttgagt cgctggaggg cgaggcggtc gctgcgcagg tcgatgccgg tctcctcctg
     5101 aaactgtttg gtcatccagc cgatgatgcg ctcgtcgaag tcttcgccgc cgaggaaggt
     5161 gttgcccgag gtggagagca cctcgaagac gccgttgttg atttcgagga tggagatgtc
     5221 gaaggtgccg ccgccgaggt cgtagacggc gatgcgctcg ttctcggtgc ggcccatacc
     5281 gtaagcgagg gcggcggccg tcggctcgtt gatgatgcgc tcgatcttga ggccggcgat
     5341 gcggccggcg tccttcgtgg cctggcgctg cgtgtcgtcg aagtaggcgg ggacggtgac
     5401 gatggcctcg gtgacggctt cgcctaagaa gtcttcggcc gcggccttga gacgctgcaa
     5461 gacgatgccg gaaatctcct gtggactgta gacgcgcccc tgcacctgga tgcgcgcgtc
     5521 gccgttggtg gaatcgacga tctcgaaggg gacggtctct ttggcgcgcg ccacctcggg
     5581 gtcgccgtgc ttgcggccga tcagacgctt gacggcgtag accgtgttgg ccgcgttggt
     5641 gacggcctgc cgcttggcga tctgcccgac gaggcgctcg cccttgtcgg tgaagccaac
     5701 cacggtcggc gtggtgcgcc ccccttcctt gttcgggacg atctgcacgg cgccgccttc
     5761 gagcacggcg acgcagcagt tcgtggtccc taaatcgatt ccaacaactt ttcccatagc
     5821 ggccttccct gaaggtgaga cgttcgtgac ggtcgggggc aagcgcgggg ccgcagtcgg
     5881 ctttacttcg cgaccttgac catcgccggg cggatcagct tatcgcccag acgatagcca
     5941 cgggtcaact cctcgatgac ggtgtccggc tcgtgtcggt cagacgactc ggcggccacg
     6001 gcctcatgga cgtgcgggtc gaagggctgc ccgacggtca gaaccggctt gacgccgagg
     6061 gcttcgagca cgccgcccaa ctgacggttg atcagctcga cgccttgcag gaagtggcga
     6121 aactcttcgg actcgcccgc ctggacagtg gcctcggcgt cgagggcgcg gcgcaggttg
     6181 tcgatgacgg ggagcagttg gccgaccacg tcgccggccg cgcggttgta ggtctcggcg
     6241 cgctcgcgct caatccgctt gcggtagtta tcgaaatccg cctgacggcg cgtgatacgg
     6301 tctagcaagt cggcgtgctc ggccctgatc ttttcgagtt ccgactcgac cctcttcaac
     6361 tcggcccgcg tggcgatgag ttcggccagc acggggccgc cttcgccgcc ggcgtggcgc
     6421 gcggtggtgt cggcggcgtc ggaggcggcc gccgcgttgc gggcctcggc gtcgccctcg
     6481 cggtgcgagg ggttcgtgtc ctgctcctca cggcgttcgg gcaggtcggc ggggtcgggg
     6541 cctccggccg tgtcggcgtc gtcgccgcct tccgccccgc ggtcgatgcg ttgttggact
     6601 tccgtctcgc cctcgtaaga agactcacgc ccgatctctt cgggcgtcag gtcgtccgcg
     6661 cccgactcgt cgcgcggctc tccttccttg ccctcttcgt caacgaagcg aaccgggatg
     6721 cggctcggat tctgtctgtc cttgttcatc atgctcgaca ttgcccttcg gaactgcccc
     6781 ggtgcggggc gggggacgcg cggcccgtcg gggccgcgcg gcgtgtcaga tgatcgcggc
     6841 ctcatcgcgc agcgtccgtt cgaagatgtg cgccacgtag ctgacgacgg ccatcgtgcg
     6901 cgcgtactcc atccgcatcg ggccgacaac gccgacgctg cccgccgcgt caccgccgcc
     6961 aacgcgataa ggcgcgctga tgagcgcgca cgcacgcagc gacggcgcgc tatgctcgcg
     7021 gccgatgacg acgtgtacgt cgccgaagac cggctcgcgc gcgatgcact cgttgagtat
     7081 tttgaccaga cgcgacttgg cctcgaaagt ctggaacagc tcgcgcaatc gctcgatgtc
     7141 ggagaagtcg ggcttggcga ggatgttcga ggcgccgtcg agatagacct cacccgcatc
     7201 ggactccccg ccgtcgagac tcttctcgca caggagcgcg acgttttgga gcagctcgtc
     7261 gtagagcgcc ttctcctcgc gcatcagcgc gagcacctcc gcgcgaatgg actggaggct
     7321 cttgccgctg aactccgtgt tcaggtagcg cgcggcgcgt tcgagttcgt cctgcccgag
     7381 cggctcgtca acgcggatca gcttgtcctg cacgacgccc gaggcgaaga cgagtacggc
     7441 cagaacgcgg ccgtcggcga gccggaggaa ttcgatgtgc tggagccggt tgtcggcgag
     7501 cggcggcgag accacgatcc cgacgttctc cgaaagctcc gagagcaggt gcgagacgcg
     7561 ttccatcagg cgttgcggcg aagcgccgtt ctcaagattt gtcagcccga gcgtccggtc
     7621 gatggccgcc atgtcggcgc gcgacagacg cgtgcccgac gcgaggtgat cgacgtagaa
     7681 gcgataaccc gtgtccgtcg gcacgcgccc cgccgaggtg tgcggctgct cgacgagtcc
     7741 cgcctcttcg agttccgcca tcacgttgcg aatcgtcgcc gaactccacc cggcgccgcc
     7801 ggcgaagccg tccgcaatcg tcttcgaccc gacgggttcg cccgtccgca gatgctccct
     7861 gatgatggcc gacagcacgg cctgcccgcg cgcgccgggc ctccattccg cctgcttcgt
     7921 cgaacctcga attgccatca gaaataaagg gcgtggggga ttgaatgatg cgggtaaact
     7981 gccatggccg gacagggaca gacgcccggt tgtttaattg tttacagtcc cgcaaaccca
     8041 ctgtcaagat ataatttcaa gccaaggggc cgcacgccgg agactctaaa ttatgtccca
     8101 cggcagtata acggattgag caaatgagga gcaaaccggc ctgttcgcaa acggaacagc
     8161 ccgcccactt ccgcccccat tcgacggtta atttactgcc agcgctacag ttataatctc
     8221 gtcgcggctc gtccgtcacc ccggcatcta agctgctcac cttatacgac ttacaggccg
     8281 cgctcgacga cccttcggcg gcgactggtt ttcccatgct gacattgtat agagacgcaa
     8341 gggcttagcc gtctctccaa agagtcgatt gacgccctca caaacctcca cgtaatatca
     8401 gtgccactga accgtcgttg tccagcctgc tcttcgggga tgaaattgac ctggcaagct
     8461 tattactaac tgccggcgca cggcgcgctc cctcgcagct ttgactctta tgcaagactc
     8521 acagcccctt ctgcacacgc tctttctcga cgcgtttcgg cagctcgcgc ccagccgccc
     8581 cgcgcccgac gtggacgttc gctactaccc ttacgccggc ctcaaccaca cgatccgcct
     8641 ccgcacgggc cgcgtctacg tgcgcgtctc cgacatcttc cgcggcgcgc ccgacaacgt
     8701 gcaccgcgcg ctggccttca tcctcgtcgc caagctcctg cgccggcgca cgccgcaact
     8761 gcacgagcgg acttatcgca actacgcctg ccagcccgaa gtgctgcgcg cctccgacct
     8821 cgcgcggcgc gagcgcggac gcaagcaaat ctcctccgcg cgagggagcc gctacgatct
     8881 cgaaaagatg ttcgcgcgtc tgaaccgacg ctacttcgac ggcgagttgc cgccgcccgt
     8941 cctcacctgg tcgcgccgac gcacgcgcac catcctcggc caccacgacg gcgtccacga
     9001 gaccatcgtc atcagcaaga ccctcgactc ggcggacgtg cccgagtggt tcgtcgagtt
     9061 catcctctat cacgagatgc ttcacatcaa gcacccggcg cgcctcatca acggccggcg
     9121 cttctaccac accaaggcct tccgctccga ggagcagcga ttccccttct acagcgaggc
     9181 gcaggaatgg ctcgaccgca tcgcgcgaca gcgccgttcg ttgcgtgcgc gcgccgcgtg
     9241 aaattgaatt tccgtctaac attttggccg aaaattttag gaaactttcg cacccgcgtt
     9301 gcgtttaaac gctcaggaag gtgggctcct ccgaaagggc gggtcgagcg attcagacgg
     9361 catacgattt ccctccgtgc tgtctgagtc gcccgacccg cccttcaggt tatgcccacg
     9421 tttattttca aacgcacccg cgcctcacac tttcactccg caggcgacgc actgagtttc
     9481 gctttcttga cactctcaaa tccagcgtgt tagcgtccgc actgttccac gaacacaggc
     9541 catcaaccga ttcattccag accgtggatt ttaatcccga gtcctgtcag gagacgttta
     9601 cgtgagcgac aaagtcttca agtccgtcga gatcgtcggc acatccgaac agagttttag
     9661 ccacgccatc gaggtcgcgg tcgagcgcgc gcgacaaacc ctgcgcgagc tttcctggtt
     9721 cgtcgtcggg gagatgcgcg gcggtttgca gcagggccgc ctcgaatatc aggtgacgct
     9781 gcgcgtcttc ttcaagctcg acgccgccgg ggggcagggg ccggacggcg acctcgcgtg
     9841 agccgtccgg cgctacgctg ccgctggaca aagctgcgcg ccgctcgcta gactacgcgg
     9901 tttgtgacag gggcttcgga gcaatcctcg cgcgttgggg cttcaacaga aggtaaacat
     9961 ctcgacacgg agcacatatc cgacatggct tttccggcaa cacagatcag gcgcggcatg
    10021 gttatcctct tcgagggcgc gccctgcaag gttctcgatg cgcgccacca cacgcccggc
    10081 aacctccgcg cgatgatgca gacgaaactg cgtaacctgc gcaccggcag cagcttcgag
    10141 caccgcttcc gctcggccga cacggttgag cgcgcccacc tcgaacagca cgagatggag
    10201 tacatgtact ccgacggcgc gcaccatcac ttcatgaaca ccgaaaacta tgagcagacg
    10261 gccctgagcg gcgaagaact cggcgacatg gcgcagtggc tcacggcggg cctccgcatc
    10321 gaagtggagt tctacgacgg cgcgcccatc ggcatcacgc tcccgccctc gctcgaactc
    10381 gccgtcgtcg agaccgaacc cacgatgaag ggcgcgaccg tctccaacgt caacaagccc
    10441 gccaagcttg aaaacggcgt caccatcctc gtgcccccct tcgtcaacga gggcgaccgc
    10501 atccgcgttg accccatcga gggccgctac atggaacggg caaaatgaaa agtgataagt
    10561 gatgagtgat aagtgataag aaaccccgtc tgttctttct tatcacttat cactcatcac
    10621 ttatcactta tcactgatgt atctcttcta cagcatcctc ctgaccttcg gcttcgccct
    10681 cctcctccct cgcttccttt tcgacgccct ccgccacgga aaatacgcgg ccggctttcg
    10741 ccagcgcatg ggcagtctgc caagcctgtc ggcgggcggc gcccagaagc cggtcgtctg
    10801 gctgcactgc gtctcggtgg gcgagacaca ggcggcgcgc ccgctcgccc gcgccgtcct
    10861 cgaacacttc cccgcgcacg cgctggtcgt ctcgacgacg acgctcacgg ggcaacgcgt
    10921 cgcgcgcgag gcgttcgccg gcatggcggc cgaggtcttc tacttcccct tcgactgggc
    10981 gtggagcgtg cggcgcgcgc tccggcgggt gcggccttcg gcggtgctcg tgatggagac
    11041 ggagctatgg ccgcgcttct tccgcgagtg ccggaaggcg gacgtgcgcc tcgccgtcgt
    11101 caatggccgc atctccgaga actctttccg ccgctaccgg ctcgtccgac cgttcatcag
    11161 ccgcgtcctc aacgacctcg acctggccgt catgcagtcc gaatccgacg ccgagcgcgc
    11221 ccgtttactt ggccttgttc ccgacaaggt cagggtttcg ggcaacatca agttcgattt
    11281 tgaaatcgac gcggcggagc agacgctgac cgctgacctg cgccgccggt tcggcttcga
    11341 tgatgagggc cgcccgctga tcgtggccgc gagcacacac gcgcccgagg agcgcatcgt
    11401 gcttgacgcc ttcagggagc ttcgcggcgc gggcgccggg cgtgggccgc gtcttctcgt
    11461 cgccccgcgt caccccgaga ggttcgcgga ggtcgccgca ctcctcgatg cctccggctt
    11521 cgcgtgggcg cggcgctcag ctacgccgct tccgaccgac gctcaggccg acgtggtgct
    11581 gctcgacacc gtcggcgagc tgcgcgcggc gtacccgctc gccgaactcg tcttcgtcgg
    11641 cggcagcatc gcgcaggtgg gcgggcacaa cgtcatggag ccggcggcgt ccgcccggtg
    11701 cgtcgtcacc ggcgcgcaca cctcgaactt ccgggccgtc gtcggcgctt tcctcgaacg
    11761 cgacgcgctc ctccagctcc cgccgctcgc ggagagcgag gccgcccacg ttctggccga
    11821 gacttttaga aacctcctcg aagacgcgga tcgcaggcgc cgcgtaggcc aaaatgcccg
    11881 cgccgtgctc gacgagaatc gcggcgcgac ggggcgcaca atcgcgctgc tcgcgcccat
    11941 cttcgaggcg aggcgtgagt ccgccggcct gcgctccgca cgggggcgcg ggctggcctc
    12001 tgatctccgc ccgcccctcc catgaacacg tcattcgccc tcgcgccgct cagcgcgctc
    12061 tatggcgcgg ccgtccgcgc gcggctcgcg ctctatcgcg gcggcgcgct caggcaggag
    12121 cgcgcgggcg cgcccgtcat cagcgtcggc aacatcaccg cgggcgggac gggcaagacc
    12181 ccgcttgtcg agtgggtcgc ccgcgccgtc gcgcgcgagg gccgccgagc gtgcgtgctg
    12241 acgcgcggct atggcaggcg cgacgagcgc ctccgcgtcg tcgtctcgga cggcgaacga
    12301 ttgctcgccg acgcgcccga ggccggcgac gagccgcgca tgctggccga acaactgttg
    12361 ggtgtggcgt cggttgtcag cgacgccgac cgcgcggcgg ccgccagatg ggcgctcgaa
    12421 aatctccgcg cgcaagcctt cgtcatggac gatggcttcc aacacctgcg actcgctcgc
    12481 gacctcgacg tggtggcgct cgacgccacc gcgccgtggg gcggcgggca tctattgcca
    12541 cgcggtcgcc tgcgcgagcc gcgccggggc atcggccgcg ccgactgcgt cgtcatcacg
    12601 cgcgccgacc tcgccggcga cgccgccgcg cgcgccctgc gcgaagaagt ctcgggcctg
    12661 tgcggcggcg cggcgctcgt cgtcgaagcg cgcgcgcgca cgacgggctt gcgccgtctc
    12721 gctgacgaca cggccgggga cgagtttgtc agtgcggccg acgtgcgaag cgtcggcgcc
    12781 ttctgcgccg tcggcaaccc gcaatcgttc tttgagcacc tgcggcgcga cggccacgaa
    12841 ccggttctcg cgcgcgcctt cacagaccac cacgcctaca cgcagtccga cctcgactcg
    12901 ctcacgcgcg ccgccacaga ggccggggcg cactcactcc tgacgacggc caaagacgcc
    12961 gtcaagcttc gccgtttgag cacgaagctc ccgatctacg tcgtggaaat tgcgttggaa
    13021 attgatgagg aagagaagct gctcgaactc gtgcgcgggg cgctcgcgaa gagttgagct
    13081 ggcgggccgc aggcgcgaga tggcgcgtcc tcgcgtcgtt aatcagagtc ggccgggggg
    13141 gacgccgctc gttcccgcgg gcgcgggcac gtcgccggag actggtggcg gtggtgtggg
    13201 cgcgggcttt ctcggcggcg gagcctcgtc agggtcaggg ttcgcgttta ctggcggcgg
    13261 cggggtcggg gcgcgccggt tcgtgtttct ctcaggcggg gtgggttcgg gggtcggctc
    13321 ggcctcttcc tcctcgccct ctgtgttcgc gttctcgttc cgcccggcgt cgcctccggc
    13381 gggcgtgggc gccacgccga ggttggcgtt cgcgttgccg ctggccggaa tggtttcacc
    13441 ggcgataacc tcgcccggcg tcggggtggt gagtccggtc gaaggcgtga tgttgctttc
    13501 gcccgcgcca gtcggcggcg tgacggagac gacgggctgg ctgttcgggt cgctcgcgag
    13561 cggcggctgc gtttcgcccg ccgtgtcatt cccctggaac aggtagaaca aaccgaagac
    13621 ggccgcgagt acgatgaggg ccgggatcgc gatgcgccac gggttgaagc tggcggccgg
    13681 cggcggcgga agcggggcgt actcctcacg cgtttcctca cgcgtccgcg tcgtgcgggc
    13741 actcacgacc gtcgcctctt cctcgtccga cgccacggcg ccggtggtgc gcggggcctc
    13801 gttctcgccg gtcggcacga cgatgcggct cgtctccggc acgccgcgcc cgtcggtcgc
    13861 cgaggcccgc gcggcggcgt tcgcggcagc ggcggcttcc gtcggtgcgt ctgcggaggc
    13921 tgcgctcgcg aacgccccgg agagttcgcc cgcgttctgg aagcgatggt cgggattctt
    13981 ggccagcgcg cgttggacga ccgcatcgac ggacgcgggc aggtcgtcgc gctccgtgtg
    14041 gagcgacggc gcagcctcct gcatctgcat catcatcacc tcggtcggcg agtcgcccgt
    14101 gaagggcacg tggccggcga gcatttcgta gacgatgatc ccgaagctgt aaatgtcgga
    14161 gcgggcgtcg atcttcgacg cccccgagca ctgctccggc gacatgtact gcggcgtgcc
    14221 gatgatcagg ttcggcgcgg tgagttccgg gtcctggttc tcggccgtct ccttaatctt
    14281 ggcgatgccg aagtcgagca ccttcgccca gtcgccgccc ggcgtctcgt cgagcatgat
    14341 gttatcgctc ttgagatcgc gatgcacgac gccttcgttg tgcgcggctt cgagtgcgcc
    14401 ggccatctga cggacgatct cggccgcacg ctttaagggc atcggcccct cggagcgaat
    14461 cacctccttg agggtgcggc cgcgcaggta ctccatgacg aggaagacga cgccgttttc
    14521 ggcctcgccg aaatcggtga cggtgatggc gtgcgggtgc gagatgcggg aggcggcgcg
    14581 ggcctcgcgg gtgaagcggg cgacgatgtt gtcgtcggcg gcgagcgccg ggtggagcac
    14641 cttgacggcg agggtcttct ccatcagcac gtgcgtcgcg cggtagacgc agcccatgcc
    14701 gccctcgctg agcttctcct cgatgcgata cttgccggcg agcgtttggc cgacaagctc
    14761 gtcctgccgc ttgaggacgg agccgtcgga cgggcacaag gtgttcgcgt cctcaaactc
    14821 cttgtcgcat ttcggacatt tcttcatctg cgggacaccg ccctgttccc tctcgccttc
    14881 gactgttcgg gttggcttga gcgtatcaca ccgcttccgc cgcgcgcaac ggcgactttt
    14941 cggcgcgcgg ccgggaacac ggatgagacg gatcaaaacg gataagcact gattgaatcc
    15001 gtccttatcc gttcaatccc tgctccctcc cgaccaccac tcacaatgct ctcactttac
    15061 aaaagctcaa agaccgtctt cgtccaaaaa actcgggcgc gccccgttgc aacggggcgc
    15121 gcccgagttt tgttgtcagc gagacggacg agatcgagcc tagagcttcg cgaagacgac
    15181 gacgagcgtg aagagcacga gtgtctcgat gaagacgagg ccgagaatca tcagcgtctg
    15241 gatgcgcgcg ccggcgccgg ggttacgcgc cacgccctcg gccgccgccg cggcgacgcg
    15301 cgacgggccg atggccgcca gcgccgccgc cagcgcgaag ccgaagccgg ccgccagcgc
    15361 cttgtacttg ttgatcgacg cgtcgttgtc cgccgcgccc gcctgcgcga gcgccggagc
    15421 ggccgaagcc aggacgccga gtgccgcgaa gaaagccata cggagtttgt tcatgttcat
    15481 tctcccgaag tgtttacgtc tgccagtgcg aatcagcggt cgaagttccc tgtccaatcc
    15541 tccggcgcct tcggtgttct cggtcggatg cttcttactt atgatttcgc cctcacccgg
    15601 tatccaccgc ccctcttaca acggcgctcc agcgatgacg gccctcagcc cgctttcaaa
    15661 ttgcgaaagc catgactccc gcgtccgaac actcaccccg gcctcgtcga attttccctt
    15721 tcacttcgcc cccggttcgc ttcccctctc gccccgacgc gcgccggccc gtctggcccg
    15781 cgccgcccgc cgagcgacta ctgcatgtca ataaagccga acgtctaaga atctcattgc
    15841 tgaaaactca agggtgcgcc gtcgcgtaac cgccctcgtc aacagtgtgc ccgtgctggt
    15901 cgtccgcgcc gtggtgctcg tcgccctcgt ggtgcgagac ctcggcgatg tagaggatcg
    15961 agagcagcgt gaagatgaac gtctgcatga aggcgacgaa gacgctcaaa ggcatgatga
    16021 gcaccggcac tggcagccac cacggcacga ggctggcaat cgtccccaga atttgttcgt
    16081 cgccgaagag gttcccgaac aggcgcacgc ccagagacat cgggcgcacg aggttgctga
    16141 tcagctcaat cgggaacatc aaaatggcga gccacggtat cggccccgcg aagtgcttga
    16201 ggtgcccgcc cacgccgttc tctttgatgc cgacgtagtt gtagtagacg aaggacgaga
    16261 tgccgagcgc gaaggtgacg gaggtcgagg ccgtcggcga catcaggccc ggaaagagtc
    16321 ccatcaaatt cgagacgagg atgagcacgg cgaaggtggc gacgacgggc aggtaagcca
    16381 tcccgctcgg gcccacgttg tcgagcagca gaccgcgaat cgactgcacg cccgtctcca
    16441 gcatctgctg gccgttgccc ggctcgtcct gcgacatccg gccccgcaac agccacacga
    16501 gcgccagact gatgaggcac gccagaatga acatcaccgt gtaccacggc acggcgttct
    16561 cctctgtgta cttgccgaac acgttctcgg ccgtcgtccc gaagttcgag aagaacctgt
    16621 cccacagagg cttcgtgtag tgaagctgga actcgtggac gggcttgcct atctggtggt
    16681 tgatgaactc gacgatggcc ggcgtgtggt gcgcggccgc tgccggcgcg gcgtggtcgg
    16741 cggcggcgcc ttgcgcggcc ggggccgggt cgctgaacag gaagaacatc gtttacgttt
    16801 cctctcgacg gatgacggcg aagtataact gcatgaaacc ttcaatcagc acggccgctg
    16861 ccgaggagca gaggccggcg agcacggcca cgagcgacac gaggtcgagc gcgtaagcgg
    16921 ccgtcgccac accggcgacg acgacgtagc ggagcacgta gcgcgagagt ctccatgtcg
    16981 ccctcacgcc cggcgcggtt gagccgaaca tcgccgcgac cgacgtgcgt agccagtgga
    17041 aattcaggag cgagaggaga ccgcccgtga gcagccccgt cgtcgtgcgc cacgggagga
    17101 gcgccgcgct caacagcacc gcgacggcca cggccgcgca catcccccgg aagatgcggc
    17161 gctcgaccgc cgccggctct tccccgcccc ccgcggccgg cgagttcgag tgcgcgtccc
    17221 ctgcgattac agacatagcc tttagaacgg aagattttac acgcgaccgg cgcgcgctgt
    17281 cgagcgcacc cgcctaattg ttgttcctcg acatgatcct gatgaattgg tagaagccgg
    17341 cgaccgagcc gagcaggatg cccgtgatga ttaaccaggg gccggtgccg aagtaactgt
    17401 cgagcagcca tcccaagccg aggaagctcg ccacggagaa gaagatggcc aacccggcgg
    17461 cataggccgt gctgcttcgg cgcgtgacgg ctgcctcgtc ccggctctct tcgttttgcg
    17521 agtcgtcggc cactacggct tttgccctcc ggcgcgcgtc cggtttttac ttcaacaact
    17581 gcttgttctt catgtcttcg agcacgcggt cggccctgtc caactcccag tcgggcgcgt
    17641 cccaggcgtt cagttgcgga cgcgcgggcc gcttcagaac cttgtaggcg acgccgacga
    17701 tgaccgcgag cacgccgatc cagagcagat actggaaggc ccacgcgatg aggccgaaga
    17761 cgaagaacgc cccgatgccg gcgagcagca gaacgagcag cgcgagaact atcttgaact
    17821 tatccatatt tgagacctcc ccagagccga ctagtagagc gcgccccaac ggcgacgtgc
    17881 tcacagtctt atacgccccc gcgccttccg gcgttcgtca ccacttagca cgccccgcga
    17941 aatttttcgg cacctcccac agcagtcctc gtcgcgaatg gcgcatcgag tcgtggataa
    18001 agcagacgtg cgtacaccag ca
//
LOCUS       DASVES010000512         6270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_508524, whole genome shotgun sequence.
ACCESSION   DASVES010000512 DASVES010000000
VERSION     DASVES010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508524"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF240_21785"
     CDS             complement(<1..261)
                     /locus_tag="VF240_21785"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX7177906.1"
                     /translation="MSDDSEKSGKSKRTRAVIDRVEDGGRAVVQLGDGEGDPFDLPAE
                     LLPDGASDGDHLVITVRVEGESRAAAEDRIRALQERLEKRGKQ"
     gene            complement(283..1371)
                     /locus_tag="VF240_21790"
     CDS             complement(283..1371)
                     /locus_tag="VF240_21790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX7177907.1"
                     /translation="MYQQQPAPRSSSKALKGCGCALAVAALAALAILALWWFVFRDRG
                     PQPPPASGGDLRVVVLDVEQGDSILIISPSGKAALVDAGNPGYGARVLDALRRHNVSQ
                     IDLFITTHAHADHIGAADEVIRGATVRRVLDSKVPNATRNYEDFLRAIEEKGVEYVGA
                     EPGQKYEDLGDGVVFTVLSPIQPLFTKEQVRSGGNEPNANSVVVRLDYGEFSMLLTGD
                     AEAQTEERMIRQGANVKAKVLKVGHHGSPHATSDSFLKEGAFAAAVISCGAKNLYGHP
                     DQRVLDRLRAANVKVFRTDLHGEVTITTRGEGYEIKPEREAKGDVWAGREAKKRDTAS
                     SGFIDYGDFGPPPKPKPTPRAKGAAGGR"
     gene            complement(1426..1791)
                     /locus_tag="VF240_21795"
     CDS             complement(1426..1791)
                     /locus_tag="VF240_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177908.1"
                     /translation="MAQSEFDRKIEVITGILADLTVKQQLTGEYMNDWKERMARADER
                     MDRADRRMTRVENILKLAIRAGRRERREWREKYAALVDAQKRTEESLKHLADSQAHTD
                     SRLDALIDIVREGRNGKTE"
     gene            complement(1840..3276)
                     /gene="purF"
                     /locus_tag="VF240_21800"
     CDS             complement(1840..3276)
                     /gene="purF"
                     /locus_tag="VF240_21800"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7177909.1"
                     /translation="MDDQQRDGFRDECGVFGIFGHPEAARLTYLGLYQLQHRGQESCG
                     IVASDGEQLRSERAMGLVSEVFTEKRLNRLPGSAAIGHTRYSTAGEVSIREAQPFLVA
                     CQHGQVAVCHNGNLPFATDERKELERAGAIFSSTSDTEVILHRIARSHAASVAEAVPE
                     VLRETEGAFSMLFLTPDSLIAVRDPRGFRPLALGRLGDAWVVASETCAFDLIDARYVR
                     DVEPGEMIEVTRDGRLRSTHPLPARPHSFCIFEHVYFSRPDSIIFGRSVNESRHKMGK
                     RLAVECPAEADLVVPVPDSGVAAAIGYAYQSGIPFRQGLVRNHYVGRTFIEPKQSIRS
                     FGVRVKLNPVRDLIDGRRVVLIDDSIVRGTTSKKIVGMVRDAGAREVHMRVSCPPTVS
                     PCYYGVDTPTKRDLIAAQMSVEEIRRFIGADTLGYLSLEGMLEAAGFPASDSCTACWT
                     ENYPTRITRDAETQVEREREPVPSGDVK"
     gene            3442..3696
                     /locus_tag="VF240_21805"
     CDS             3442..3696
                     /locus_tag="VF240_21805"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX7177910.1"
                     /translation="MAVAVKVYGADWCGDTTRAREYLDRLGVAYEYVDVERDEAAAEW
                     VREQNGGKERKPTIDVGGQILSVPSEHDLVGALRERGLMA"
     gene            3712..4230
                     /locus_tag="VF240_21810"
     CDS             3712..4230
                     /locus_tag="VF240_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177911.1"
                     /translation="MSESPSPLQQILAPLARAALLLALAAAGWGVYRHLPDDGPVGLG
                     RRGRGETTTLRLVLRRPPDYPTDDQQKVSVQLYSINVEAARSEYISERRPGVRFEEFV
                     TRRMAGREPVAAEFDERGEAVVVVPQGRWWIHATLDGPEELTWRLPVNVSGREKTIEL
                     GIDNAYTRAKSF"
     gene            4345..5280
                     /locus_tag="VF240_21815"
     CDS             4345..5280
                     /locus_tag="VF240_21815"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7177912.1"
                     /translation="MNLRETPAPRREGEALVRVRLAGVCNTDLEIVRGYAGFEGTLGH
                     EFVGTVEEAPAGHEGLVGRRVVGEINAGCGRCELCRAGDARHCAGRTVLGIHGRDGAF
                     AEYLSLPAVNLLSVPEEVSDECAVFTEPLAAACGVTERVLVEASARAAVVGDGKLGLL
                     CAQALKVLTGAEVTLVGRHDEKLRIAARRGVETTKAEDLKPSARRAFDVVVEASGSAS
                     GFATALTLIRPRGTLVLKSTFHGTTEFNAAPIVVDEISIVGSRCGRFAPALELLRRRS
                     VDVESLITEEHALADGPRALARAAAPGVLKVLLRM"
     gene            5380..>6270
                     /locus_tag="VF240_21820"
     CDS             5380..>6270
                     /locus_tag="VF240_21820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7177913.1"
                     /translation="MKIKADLKADAVLVLTTIIWGSTFPLSKDILDHWPPVAYLVMRM
                     GVSAALMALLFRRQLARAGRAEWRAGATLGLLLAVGMTGLVVGQVYTTAARSAFIAGL
                     TTPLVPFVAYLLTRARPGVENLLGIVLASAGGLLILAPQGEAGAANTGDLLTLGCTVF
                     FAAHLTLMSVYARRYDVRALTVIQIATVSAVLTAYWLALRAAGALYGTEGLPLAVARE
                     FQPMSWEADVLWRFAYMVLVATVLNFLMWTWAQGRMSATHAAIIFSLEPVFATIFAVW
                     MRGAGEWTGGRANLGAALILA"
BASE COUNT          937 a         1992 c         2235 g         1106 t
ORIGIN      
        1 ttgcttgccg cgcttctcca gccgctcctg tagggcgcgg atgcggtctt cggccgcggc
       61 gcgcgactcg ccctcaacgc ggacggtgat gacgaggtgg tcgccgtccg aagcgccgtc
      121 ggggagcagc tccgccggca gatcgaaggg gtcgccctcg ccgtcgccga gttgcacgac
      181 ggccctgccg ccgtcctcca cgcggtcgat gacggcgcgc gtgcgcttag acttgccgct
      241 tttctcggag tcgtcgctca tggggattca cgtggtcggc agtcatctcc caccggccgc
      301 gcccttcgcg cggggcgtcg gtttaggttt gggcggcggg ccgaagtcgc cgtagtcgat
      361 aaagccggag gaggcggtgt cgcgcttctt ggcctcgcgg ccggcccaca cgtcgccctt
      421 ggcttcgcgc tccggcttga tctcgtagcc ctcgccgcgc gtggtgatgg tcacttcgcc
      481 gtggaggtct gtgcggaaga ccttgacgtt ggcggcgcgc aggcggtcga gcacgcgctg
      541 gtcggggtgg ccgtagagat ttttggcgcc gcaggagatg acggcggccg caaaagcgcc
      601 ctccttgagg aacgagtcgg aggtggcgtg gggcgagccg tggtggccga ccttgaggac
      661 tttggctttg acgttggcgc cctgccggat catccgctcc tcggtctgcg cctcggcgtc
      721 gcccgtcagg agcatcgaga actcgccgta gtcgagacgg acgacgaccg agttggcgtt
      781 cggctcgttg ccgcccgagc gcacctgctc cttcgtaaac agcggctgga tcggcgagag
      841 cacggtgaag acgacgccgt cgcccaggtc ttcgtacttc tgtcccggct ccgcgccgac
      901 gtattcgacg cctttctcct cgatggcgcg caggaagtcc tcgtagttgc gcgtcgcgtt
      961 gggcaccttg ctgtcgagca cccggcggac ggtagcgccg cggatcacct cgtcggccgc
     1021 gccgatgtgg tcggcgtgcg cgtgcgtcgt gatgaagagg tcgatctgag agacgttgtg
     1081 acgacgcagg gcgtcgagga cgcgcgcgcc gtagccgggg ttgccggcgt ccacaagcgc
     1141 cgccttgccc gagggcgaga taatcagaat cgaatcaccc tgctccacat cgaggacgac
     1201 gactctcagg tcgccgcccg aggccggcgg gggctgcggg ccgcggtcgc gaaagacgaa
     1261 ccaccagagg gcgaggattg cgagcgccgc gagcgccgcg acggcgagcg cgcacccgca
     1321 ccccttcagg gctttcgacg aactgcgtgg cgccggctgc tgctggtaca tagtattcgt
     1381 ttggcggttg actccttaac ccatcacacc gccccgccgg ggaagctact cggtcttccc
     1441 gttccttcct tcgcggacaa tatcaatcag ggcgtcgagc ctgctatccg tgtgcgcctg
     1501 cgagtctgcc agatgtttta acgactcctc ggtgcgcttc tgcgcgtcca cgagcgccgc
     1561 gtacttctcg cgccactcgc ggcgctcgcg ccggcccgcg cggattgcca gcttcaggat
     1621 gttctcgacg cgcgtcatcc gccggtcggc gcgatccatg cgttcgtcgg cgcgcgccat
     1681 gcgctccttc cagtcgttca tgtattcccc ggtcagttgt tgtttgaccg tcaggtcggc
     1741 caggatgccg gtgatcactt ctatcttcct atcgaattcg ctctgtgcca tctcttcgtc
     1801 gctcctttca aggtgaggaa tgaaagtgtg tttgcgaaat cacttcacgt cgcccgaggg
     1861 caccggctcg cgctcgcgct cgacctgggt ctcggcgtcg cgggtgatgc gcgtcgggta
     1921 gttctccgtc cagcaggcgg tgcaggagtc ggaggcgggg aagccggcgg cttcgagcat
     1981 gccttcgagg gagaggtagc cgagcgtatc ggcgccgata aagcgacgaa tctcttcgac
     2041 ggacatctgc gcggcgatga ggtcgcgctt ggtcggcgtg tccacgccgt agtagcaggg
     2101 gctgacggtc ggcgggcaac tcacacgcat gtgcacctcg cgcgcgcccg cgtcgcgcac
     2161 catcccgacg atcttcttcg aggtggtgcc gcgcacaatc gagtcgtcga tgaggacgac
     2221 gcggcggccg tcgatgaggt cgcgcacggg gttgagcttg acgcgcacgc cgaaactgcg
     2281 gatcgactgc ttcggctcga tgaaggtgcg gccgacgtag tggttgcgaa cgagaccctg
     2341 ccggaagggg atgccgcttt gataagcgta gccgatggcg gcggccacgc ccgagtcggg
     2401 cacggggacg acgaggtctg cctccgccgg gcactcgacg gcgaggcgct tgcccatctt
     2461 gtgccgcgac tcgttgacgg agcggccgaa gatgatcgag tcggggcgcg agaagtagac
     2521 gtgctcgaag atgcagaagg agtgcggacg cgcggggagc gggtgcgtgg agcgcaaacg
     2581 cccgtcgcgc gtgacctcga tcatctcgcc cggctccacg tcgcggacgt agcgagcgtc
     2641 gatgaggtcg aaggcgcagg tctcggaggc gacaacccag gcgtcgccga gccggccgag
     2701 ggcgagcggg cgaaaaccgc gcgggtcgcg gacggcgatg agcgagtcgg gcgtcagaaa
     2761 caacatcgag aaagcgccct cggtctcgcg caggacctcg gggacggcct cggccaccga
     2821 cgcggcgtgt gagcgggcga tgcggtggag gatgacttcg gtgtcggagg ttgaagagaa
     2881 gatggcgccg gcgcgttcga gttccttgcg ctcgtcggtg gcgaagggga ggttgccgtt
     2941 gtggcagacg gcgacctgtc cgtgctggca ggcgacgagg aagggctggg cctcgcggat
     3001 ggagacctcg ccggccgtgg agtagcgcgt gtggccgatt gctgccgagc cgggcaggcg
     3061 gttgagtcgc ttctcggtga agacctcgga gacgagtccc atcgcgcgct cggaacgcag
     3121 ttgctcgccg tcggaggcga cgatgccgca cgactcctga ccgcggtgct ggagctggta
     3181 gaggccgagg taggtgagcc gcgccgcctc ggggtggccg aagatgccga acacgccgca
     3241 ctcgtcgcga aagccgtctc tctgttggtc gtccacgctt cccttgcttt acgtcccggt
     3301 cggtgtgttg aaaggcgttc ggccttcggt cggtttagaa cacggaaggc acattgtagc
     3361 attcgggcat cgttaatccg taggggcatg gcgtgctatg atgtcggcct aaaatccggc
     3421 tcggaaaaag gaagtgtggc gatggctgtc gcggtgaaag tttacggcgc ggactggtgc
     3481 ggagacacga cgcgcgcgcg cgaatatctc gaccggctcg gcgtggccta cgagtacgtc
     3541 gatgtcgagc gtgacgaggc ggcggccgag tgggtgcgcg agcagaacgg gggcaaggag
     3601 cgcaagccga cgattgacgt gggcgggcag atactctccg tgccctccga gcacgatctg
     3661 gtcggcgcgc tgcgcgagcg cgggctgatg gcctgacgag ggcggcccaa cgtgagtgag
     3721 tcgccctctc ccctgcaaca aattctcgcg ccgctcgcgc gcgcggcgct cctgctcgcg
     3781 ctcgccgccg cgggctgggg cgtctatcgc cacttgcccg acgacggccc cgtgggttta
     3841 ggaagacgcg ggcgcggcga gacgaccacg cttcggctcg tgctgcgacg cccacccgac
     3901 taccccacgg acgaccaaca gaaagtctcc gtccagctct actcgatcaa cgtcgaggcc
     3961 gcgcggagcg agtacatctc ggagcgccgg cccggcgtcc gcttcgagga gttcgtcacg
     4021 cgacgcatgg cgggccggga gccggtggcg gccgagttcg acgagcgcgg cgaggccgtc
     4081 gtcgtcgtcc cgcagggccg ctggtggatt cacgccacgc tcgacggccc cgaagaactc
     4141 acctggcgtc tgcccgtcaa cgtctccggc cgcgaaaaga caatcgaact cggcatcgac
     4201 aacgcctaca cgcgggcgaa gagtttttaa aggcagtgat aagtgatgag tgatgagtga
     4261 tgagaaagac ggctttctta ctcttatcac ttatcactta tcactcatcc acttatgttg
     4321 gctctgcgtt atgagaacgg cgcggtgaac ttgagggaga cgccggcgcc gcggcgcgag
     4381 ggggaggcgc tggtgcgcgt ccggctggcg ggggtgtgta ataccgattt ggagatcgtg
     4441 cgcgggtacg cggggttcga gggaacgctg gggcacgagt tcgtcgggac ggtcgaggag
     4501 gctccggcgg ggcacgaggg gcttgtgggg cggcgcgtgg tcggcgagat caacgcgggg
     4561 tgcgggcgct gcgagttgtg ccgcgcgggc gacgcgcgac actgcgcggg gcgcaccgtg
     4621 ctcggcatcc acgggcgcga cggcgcgttc gccgaatacc tttcccttcc ggccgtcaac
     4681 ctgctgagcg tccccgaaga ggtctcggat gagtgcgccg tcttcaccga gccactggcg
     4741 gccgcgtgcg gcgtcaccga gcgagtcctg gtcgaggcga gcgcacgcgc ggccgtcgtc
     4801 ggcgatggga agctgggtct tttgtgcgcg caggcgctga aagttttgac gggcgcggag
     4861 gtcacgctcg tcggccggca cgacgagaag ctacgcatcg cggcgcggcg cggcgtggag
     4921 acgacgaagg ccgaagacct gaagccgtcg gcgcggcgcg ccttcgacgt ggtggtggag
     4981 gcatcggggt cggcctcggg cttcgcgacg gcgctcacgc tcatccgccc acgcggcacg
     5041 ctcgtcctca aatcgacctt ccacggcacg accgaattta acgccgcgcc catcgtggtc
     5101 gatgaaatct ccatcgtcgg ctcgcgttgc ggacgcttcg ccccggcgct cgaactgctc
     5161 cgccgccgga gcgtcgatgt cgaaagcctc atcaccgagg agcacgcgct cgccgacggc
     5221 ccccgcgccc tcgcgcgcgc cgccgccccc ggcgtgctga aagtactttt aagaatgtaa
     5281 gcagtgagca gtgagcagtg agcagtaaaa aaatcgcgcg gcgtgcgaaa aggctcaacc
     5341 tactgctcac tgctcactgc ttactgctca cttcctgcta tgaagatcaa ggccgacctc
     5401 aaggcggacg ccgtcctcgt cctgacgacg atcatctggg gctcgacgtt tcccctctcc
     5461 aaagacatcc tcgaccactg gccgcccgtc gcctacctcg tgatgcggat gggcgtctcg
     5521 gcggcgctga tggcgttact gttccgacgc cagcttgcgc gcgccggtcg ggcggagtgg
     5581 cgcgcgggcg cgacgctggg gctgcttttg gccgtcggga tgacggggct ggtcgtcgga
     5641 caggtctaca cgacggcggc ccgttcggcc ttcatcgcgg gccttaccac accgctcgtc
     5701 cccttcgtcg cctatttgtt aacgcgcgcg cggcccggtg tcgaaaacct tctgggcatt
     5761 gtcctcgcct ccgcgggcgg cctgctcatc ctcgcgccgc agggtgaggc cggcgccgcc
     5821 aacacgggcg acctcctgac gcttggctgc acagtcttct tcgccgcgca cctcacgctg
     5881 atgagcgtct acgcgcgtcg ttacgatgtg cgcgccctga ccgtcatcca gattgcgacc
     5941 gtctcggccg tgctcaccgc ttactggctc gcgctgcgcg cggccggcgc gctctacggt
     6001 acggaagggc tgccgctggc cgtcgcgcgc gagtttcagc cgatgtcgtg ggaggccgac
     6061 gtgctgtggc ggttcgctta catggtcttg gtggcgacgg tgctgaactt cctgatgtgg
     6121 acgtgggcac aggggcggat gtcggccacg cacgcggcca tcatcttcag tctggagccg
     6181 gtcttcgcca ccatcttcgc cgtctggatg cgcggcgcgg gcgagtggac gggcgggcgg
     6241 gccaacctcg gcgcggcgct catcctcgcc
//
LOCUS       DASVES010000513        11707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_983931, whole genome shotgun sequence.
ACCESSION   DASVES010000513 DASVES010000000
VERSION     DASVES010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983931"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1096)
                     /locus_tag="VF240_21825"
     CDS             complement(68..1096)
                     /locus_tag="VF240_21825"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7177914.1"
                     /translation="MKAIIFYEHGGPEVLRPEERPEPRVRADEVLVEVRACALNHLDV
                     WARGGLPGVKIPLPHILGNDIAGVVREAGELVDWVAPGDEVMLQPGVSCGHCHECLRG
                     ADNLCPDYQILGYQRDGGYAELVAAPAVNCIPKPKHLSWEEAAALPLVTVTAWHMLVA
                     RAQLKPGEDVLIHAAGSGVGSIGIQVAKLLGARVIATASTEEKLEKARALGADELVNY
                     TREDWPKEVRRLTGKKGVEVVFEHTGADTWPGSIQALARDGRLVTCGATSGYDARTDI
                     RQVFYRHLSVLGSFMGSKSELLDAMRFVERGAIRAVVDRTLPLAEARRAHELMEDRAQ
                     FGKLVLVP"
     gene            1234..1695
                     /locus_tag="VF240_21830"
     CDS             1234..1695
                     /locus_tag="VF240_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177915.1"
                     /translation="MNQNLPDDFSTLDGILHALYDSISGPAEQKRDLRRFRSLFAEDA
                     RLVRVVPADAGGLARVTVFDPDEYFKAACEYFKQSGFFDREASRRVDGFGHVTHVFST
                     YESRHFADDPEPFARGVNSIQLFHDGARYWFVNIFWDFERPGNPIPEEHLS"
     gene            complement(1779..3167)
                     /locus_tag="VF240_21835"
     CDS             complement(1779..3167)
                     /locus_tag="VF240_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177916.1"
                     /translation="MNIATPAATAAATVATAAAVRREQLRPLLENLDLAAKDLDIRRP
                     DEDLPNELPISKLRRGGRRRPPDDSTEMITLTEEDGFLQWEEGAGVVMTGGRRGLRRG
                     LAPAAPSGEIVAQYKFEKLAPSDVGTFLEGLDKKWTPTPGLRRLPSAKGAPQTVAAPT
                     PNAAKKTLLFIHGTFSNNDHLLAELRSTPAGRDLIGRSLKHYGEVLFFDHPTLSVSPV
                     LNALDLARLFAGFKRPVDIVCHSRGGLVARWWLEGFSGAAVAPRRVVMVGAPLAGSSL
                     AAPPRLRAALGLITNVGRALSLAGSASSLAVPMLSVAVGLVKVITSIASLGEKAPLID
                     AAVAMIPGIAGQSRVGNSAELRRLREKTGGPLPDYFAVRCNFETDENHGWAFWRHFRK
                     ENLADLGANKVFDGANDLVVDTDSMTDLFDQIHIPAANVFDFGTTDRVHHTNYFQQPE
                     TIDFISKSLGIP"
     gene            complement(3164..5689)
                     /locus_tag="VF240_21840"
     CDS             complement(3164..5689)
                     /locus_tag="VF240_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177917.1"
                     /translation="MATKKASRAAKGGAAATAKGGAAARKGGAGKKSVSAQARSSSKK
                     RPASKKSPPARGGSGAVVAPVSLAGFVEAGAPPETGGRVSITVRTPQGLNLELRADRK
                     QFQRAAMQWSYAVRNRRRWAERESSRNEQAQRSLQQLQEWGVKETQLASLAEAEMVEV
                     SVPFKSEESSWEARIFPWEYLLTAATRELRQGRPMTVLRHLDRRSGAAAPAAASPPKS
                     LLFVESAPENLREAFTFASERELVKENLGLTAHELIDPTAETLRAEVGRQKPHVVHLA
                     GVDNHQATQLITSLKTVEAPPDGYVMAGSVDMVDAERLAGIVNAASRKPALVSCNFYN
                     SATRVGSLIVAGGADAAIGFQDEFNDALTEFFFTNFYHLWRLSNWNTAEAFRLTCQML
                     RKQGGLHGAGVVLWSARSLVAPQQPGATLRRGARRVPAPTAAGDREMVKQVEEAKEVE
                     ITLKSVGGSAARLFKEIKVVPFKSLNYSMLHNDRDLFETFLIKKRKTGTVRIKVEVIL
                     YAGPMTFPYTTTVPMKEWVLDLRKHIRVPLIYSTGPAISESVHTSLYVKVSWEGQELY
                     NDTHRVTLLPLDEWRDDDTDRVWLPSFVLPRDRAVNRVVDAGQRYLWALADDSGAGFD
                     GYQSVVEERDRVTKEVTFDTDGVDLQVQALWAALAFEMNLGYINPPPSYNLSAQRVRT
                     PSEIIDGHRGTCIDLALLMATCCEYIEVYPVIFLLQGHAFPGYWRSDVYHQSFITEMA
                     NAPVIGDDMASPQPQGPNAPVQREAWYLDKDFYEEVVQLVHDRKLVPIESTMIARRGS
                     FGDAVDAGIKNLRSKRQFHSLLDVRLARDNRVTPIPLKGGRPE"
     gene            complement(5994..6932)
                     /gene="lipA"
                     /locus_tag="VF240_21845"
     CDS             complement(5994..6932)
                     /gene="lipA"
                     /locus_tag="VF240_21845"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7177918.1"
                     /translation="MSRELRVISNAPTPQRPPRAPKPEWLKVRIGDPANHNYLLGLVE
                     KLNLHTVCQEARCPNIFECWADRTATFMIGGDICTRHCGFCAVGKGAPGRLDPAEPRH
                     VAEAVQTLDLAHAVITSVNRDDLADGGAAHWAETITQIRALNPECRVEVLIPDFNGDE
                     AALDTVLDAAPDVLNHNTETIARLYKRVRPDARYEQSMTLLRRAAERRDREGRGMLTK
                     SGIMAGLGETFDEVVELMRDLRASSCDIMTIGQYLQPHARRLPVERYVTPDEFARWRE
                     VGMSLGFKHVESSPLTRSSYHARQQTDQGIGAETQA"
     gene            complement(7284..8246)
                     /locus_tag="VF240_21850"
     CDS             complement(7284..8246)
                     /locus_tag="VF240_21850"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX7177919.1"
                     /translation="MLTFELIREAQERIRPHVHRTPVFTSRAFNEAAGRPVFFKCENL
                     QRAGVFKMRGATNKIRALTDDERRRGVVAFSSGNHAQSVALAAHEAGVRAVIVMPTDA
                     PRPKLAATRGYGAEVVTYDRQQDDRERLARDISEREGLVLVPPYDDFLIMAGQGTCAL
                     ELLEDAPDIDALLTPCGGGGLFAGASVVVKTLNSKIRCFPVESELSDDTRQSFRKGER
                     VCIPPPPTIADGMRTQSPGALTFPVVQRNAEDVLTVSEDEIVETMRFLLFRMKILVEP
                     TGAVAAAAVLFGKLPADVLQPAVVLSGGNVDPEVLARVVAGSLS"
     gene            complement(8233..8940)
                     /gene="lipB"
                     /locus_tag="VF240_21855"
     CDS             complement(8233..8940)
                     /gene="lipB"
                     /locus_tag="VF240_21855"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7177920.1"
                     /translation="MAQRTLKVRRLGRLEYDAAYRIQKETEAAVKARAQADTLLLVEH
                     PHVLTVGRRATRASIFASEEELEARGVAVHESNRGGKLTYHGLGQLVGYPVINLSPER
                     EDVHRYVRDIEEVLMRVLYDFRISGFRIEGLTGVHTAAGKVAAIGVHISRWVTTHGFA
                     LNVNTDLDYFKLILACEGEPVTSMRDILGRDVTLRAVEDRVVARFAEVFGAEPEEVPG
                     ADGAPLSGELITTHVDV"
     gene            9197..9733
                     /locus_tag="VF240_21860"
     CDS             9197..9733
                     /locus_tag="VF240_21860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177921.1"
                     /translation="MKRLYPLLLAISLLLLTPVIAGAQSRTRRSSTPQRRRAPASTPA
                     RGSNDAVVLGRLKVAEKIKIISEFLYVYGGVANQVAADEAQARQSGATAEYASVADRS
                     RASLRKSLLAVRDGLDQLEAEFRTSPDLQRYYNRLAGVAAGAADAEELAASNQLKPAG
                     RKLVQVVSQLADALAAMN"
     gene            complement(9823..11088)
                     /locus_tag="VF240_21865"
     CDS             complement(9823..11088)
                     /locus_tag="VF240_21865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7177922.1"
                     /translation="MKLSQARRGAKNLSVLTLVAVVCVALASVLHTPASAARSQTRRS
                     DVAGATQQPTPIVVPRLPPAAAPSPTPDWDNPPPPRRAPTPQPTPRESKPADAIEDDE
                     VIKVSSNLVVVPTSVVDAAGNPVQGLQPADFRLEEEGRRQEVAEVGAAEAVPLDIALV
                     FDVSSSTIKNFDFMKQAAAGFLKEVMKPSDRAAVFTIGERSNLIQPLAGADDAARKLT
                     TIPAADKATGTAFYDTVTAAAKYLAQNASGKNRRVVLVISDGEDNFSIDVRDATIKAL
                     DRANKAGDEKTAGEKLETVGEGTAGVHRRALQRVMREVQQADTVFYSINPSGPGVRLN
                     VISQRAEYGMQQLSEATGGSSFLPAQVTELDAIFRRIAAELRAQYLLQYYSSSDAAPG
                     KFLKIRVTTPARADLRVRARQGYYVQRQK"
     gene            complement(11201..>11707)
                     /locus_tag="VF240_21870"
     CDS             complement(11201..>11707)
                     /locus_tag="VF240_21870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7177923.1"
                     /translation="NAARGGAKAAQETADAAVSGVRATNERISALDDYEAQQGVTLNF
                     RVNSAVLSPDAKTQLDQIAQQALNAKGYVIEVSGHASADGATDHNRRLSQKRADTVVR
                     YLAENHRIPLRRIITPFGYGESQQVAENTSRTGREQNRRVEVKVLVNRGLLQGAPQMN
                     TPSTQNPD"
BASE COUNT         1792 a         3810 c         4037 g         2068 t
ORIGIN      
        1 ctgtatcttg tgacacagac gccctccctg acggtcgggc ttccgcccca aacctcatgc
       61 ccgcgcgtca cggcacgagg acgagcttgc cgaactgcgc gcgatcctcc atcagctcgt
      121 gcgcgcgccg cgcctcggcg agcggcagtg tgcggtcaac gacggcgcga atcgccccgc
      181 gctcgacgaa ccgcatcgcg tctaacagct ccgacttcga ccccatgaac gaaccgagca
      241 cggagaggtg ccggtagaag acctgccgga tgtccgtgcg cgcgtcgtag ccggaggtgg
      301 cgccgcaggt gacgaggcgg ccgtcgcgcg ccagagcctg aatcgagccg ggccacgtgt
      361 cggcgcccgt gtgctcgaag accacctcga cgcccttctt cccggtcaac cgcctgacct
      421 ccttcggcca gtcctcgcgt gtgtagttga ccaactcgtc cgcgccgagc gcgcgggctt
      481 tctccagttt ctcctcggtg gaggcggtcg cgatcacgcg cgcgcccaga agcttcgcca
      541 cctgaatccc tatggagccg acgccgctcc ccgccgcgtg aatcaaaaca tcctcgcccg
      601 gcttcaactg cgcgcgcgcg acgagcatgt gccacgccgt cacggtgacg agcgggagcg
      661 cggccgcctc ctcccagctc aagtgtttcg gcttcgggat acagttgacg gccggggcgg
      721 cgacgagttc ggcgtagccg ccgtcgcgct ggtagccgag gatttggtag tcggggcaca
      781 aattgtcggc cccgcgcaga cattcatggc aatgaccgca actgacgccg ggctgcaaca
      841 tcacctcgtc gcccggcgca acccagtcaa ccagttcgcc ggcctcgcgc accacgcccg
      901 cgatgtcgtt gcccagaata tgcggcagcg gaattttgac gccgggaagc ccgccgcgcg
      961 cccacacgtc taaatggttc agcgcgcagg cgcgcacctc gaccagaact tcgtcggcgc
     1021 gcacgcgcgg ctcgggccgc tcctccgggc gcagaacctc cgggcctccg tgctcgtaaa
     1081 agatgattgc tttcatggtt ggagatttta gttttttgtc gcgggatttg gaaacgcggg
     1141 gcgcgaaact cgaatcacag tccttgaact attcatccac gcccgtatcg acaggcccga
     1201 cgggcgcgcg cgagcgagcg cgaggcaaac aacatgaacc agaatctccc cgacgacttc
     1261 tcgacgctcg acggcatcct ccacgccctc tacgactcga tctccggccc cgccgagcag
     1321 aagcgcgacc tgcgccgctt ccgctcgctc tttgccgagg acgcgcgcct tgtccgcgtc
     1381 gtccccgccg acgccggcgg cctcgcgcga gtgaccgtct tcgaccccga cgaatacttc
     1441 aaggccgctt gcgagtattt caagcagtcg ggcttcttcg accgcgaggc cagccgccgc
     1501 gtggacggct tcggccacgt cacgcatgtc ttcagcacct atgagtcgcg ccacttcgcc
     1561 gacgaccccg agccgttcgc ccgcggcgtc aacagcatcc aactcttcca cgacggcgcg
     1621 cgctactggt tcgtcaacat cttctgggac ttcgagcgcc ccggcaaccc catccccgaa
     1681 gaacacctct cgtaagtcaa aagtaaaaag gcaaaaggca aaagtgaaga ggcaacctgc
     1741 ttcctcactt ttgccttttg cctttttact tttgacttct aagggatgcc gaggctcttg
     1801 ctgatgaagt cgatggtctc gggctgttgg aagtagttcg tgtggtggac gcggtcggtg
     1861 gtgccgaagt cgaagacgtt ggcggcgggg atgtgtatct ggtcgaagag gtcggtcatc
     1921 gagtcggtgt ccacgacgag gtcgttggcg ccgtcgaaga ctttgttggc gccgaggtcg
     1981 gcgaggttct ccttgcggaa gtgtcgccag aaggcccagc cgtgattctc atcggtctcg
     2041 aagttacagc gcacggcgaa gtaatcgggc agggggccgc ccgtcttctc gcgcaggcgg
     2101 cgcagctcgg cgctgttgcc gacgcgcgac tgcccggcga tgccggggat catggccacg
     2161 gcggcgtcga tgagcggggc cttctcgccg aggctggcga tggaagtgat gaccttgacg
     2221 aggccgacgg cgacgctgag catcgggacg gcgagcgagg acgcgctgcc ggcgagcgag
     2281 agcgcgcggc cgacgttggt aatcagaccg agcgcggcgc gcagacgcgg cggcgccgcg
     2341 aggcttgagc cggccagcgg cgcgccgacc atcacgaccc ggcgcggggc caccgccgcc
     2401 ccgctgaacc cttcgagcca ccaccgggcg accagacccc cgcggctgtg gcagacgata
     2461 tcgacggggc gcttgaagcc ggcgaagagc cgcgcgagat cgagcgcgtt gaggacggga
     2521 ctcaccgaca gagtagggtg gtcgaagaaa agcacctcgc cgtaatgctt gaggctgcgc
     2581 ccgataagat cgcgccccgc cggcgtgctc cgcagttcgg cgagcaggtg gtcgttgttg
     2641 ctgaaggtgc cgtggatgaa gaggagcgtc ttcttcgccg cgttcggggt cggcgcggcg
     2701 accgtctggg gcgcgccctt cgcgctcggg aggcggcgca ggcccggcgt gggagtccac
     2761 ttcttgtcga gaccttcgag gaacgtgccc acgtccgagg gcgcgagctt ctcgaacttg
     2821 tactgcgcga cgatctcgcc cgagggggcc gccggcgcga ggccgcggcg gaggccgcgc
     2881 cgcccccccg tcatcacgac gcccgcgccc tcctcccact ggaggaagcc gtcctcctcg
     2941 gtgagcgtga tcatttccgt gctgtcgtcg ggcggccgcc gccggccgcc gcgccgcagc
     3001 ttgctgatgg gcagctcgtt cggcaggtct tcgtcgggcc ggcggatgtc gaggtctttg
     3061 gcggcgaggt cgaggttttc gagtagcggc ctgagctgct cgcgtcgcac cgcggcggcg
     3121 gtggcgacgg tggcggcggc ggtggcggcg ggggtggcga tgttcattcc ggcctccctc
     3181 ccttcagagg aatcggagtc acgcggttat cgcgcgcgag gcgcacgtcg agcagcgagt
     3241 ggaactgccg cttgctgcgc aggttcttga ttcctgcgtc aacggcgtcc ccgaagctgc
     3301 cgcggcgcgc gatcatcgtg ctctcgatgg gcacgagctt gcggtcgtgc acgagttgca
     3361 ccacctcctc gtagaagtcc ttatcgagat accacgcctc acgctgcaca ggggcgttag
     3421 ggccttgcgg ctgtggcgac gccatgtcgt ccccgatgac gggcgcgttg gccatctcgg
     3481 tgatgaagct ctggtgataa acgtcgctgc gccagtagcc ggggaaggcg tgcccctgaa
     3541 ggaggaagat gaccgggtag acctcgatgt actcgcagca ggtcgccatc agcagcgcca
     3601 ggtcgatgca ggtgccccga tgtccgtcga tgatctcgga gggggtgcgg acgcgctggg
     3661 cggagaggtt gtagctcggc ggcgggttga tgtagccgag gttcatctcg aaggcgagcg
     3721 ccgcccagag cgcctgcacc tgaagatcga cgccgtccgt gtcgaacgtg acctccttcg
     3781 tgacccggtc gcgctcctcg acgacgctct ggtagccgtc gaagccggcg ccgctgtcgt
     3841 cggccaaggc ccacaggtaa cgctggccgg catcgacgac gcggttgacg gcgcggtcgc
     3901 gcgggagcac gaacgagggg agccagacgc ggtcggtgtc gtcgtcgcgc cactcgtcga
     3961 gcgggagcag ggtcacgcgg tgcgtgtcgt tgtaaagctc ctgcccctcc caggagactt
     4021 tgacgtagag gctcgtgtgg acgctctcgc tgatggccgg ccccgtcgag tagatgagcg
     4081 gcacgcggat gtgcttgcgc aggtcgagca cccactcctt catcggcacc gtggtggtgt
     4141 aggggaaagt catcgggccg gcgtagagaa tgacttcgac tttaatccgc acggtgccgg
     4201 tctttctctt cttgatgagg aaggtctcga acaggtcgcg gtcgttgtgc agcatcgagt
     4261 agttgaggct tttgaaaggc accaccttga tctccttgaa gaggcgggcg gcactgccgc
     4321 cgacgctctt cagggtgatc tcaacctcct tcgcctcctc gacctgcttg accatctcac
     4381 ggtcgccggc cgccgtcggc gcgggcacgc gccgtgcgcc gcgccggaga gtggcgcccg
     4441 gctgctgcgg ggcgacgagc gaacgcgcgc tccagaggac gacgcccgcc ccgtgcaagc
     4501 ccccttgctt ccgcagcatc tggcaggtca gccggaaagc ctccgccgtg ttccagttcg
     4561 agagccgcca caggtggtag aagttcgtga agaagaactc ggtcagcgcg tcgttgaact
     4621 cgtcctggaa gccgatggcg gcgtccgcgc cgccggccac gatgagcgag ccgacgcgcg
     4681 tggccgagtt gtagaagttg caggagacga gcgccggctt gcgcgacgcg gcgttgacga
     4741 taccggcaag gcgctcggcg tccaccatgt ccacgctgcc cgccatcacg taaccgtcgg
     4801 gcggcgcttc gacggtcttg aggctggtga tgagctgcgt cgcctgatgg ttgtccaccc
     4861 cggccagatg caccacgtgg ggcttttgcc tgccgacctc ggcgcgcagg gtctcggcgg
     4921 tcgggtcgat caactcgtgc gcggtaagcc ccaggttctc cttcaccagc tcacgctccg
     4981 acgcgaaggt gaaagcctcg cgcaagtttt cgggcgcgct ctcaacgaag aggaggcttt
     5041 tcggcgggga ggcggcggcg ggggcggcgg cgccgctacg gcggtcgagg tgacgcagca
     5101 cggtcatcgg ccgaccctga cgcagctcgc gcgtcgccgc cgtcagcaag tactcccagg
     5161 ggaagatgcg cgcctcccag ctcgactcct cgcttttgaa ggggacgctg acctcgacca
     5221 tctcggcctc ggccagcgac gccagttgcg tctccttcac accccactcc tggagttgct
     5281 gcaggctccg ctgtgcctgt tcgttgcgcg aggattcccg ctccgcccag cgccggcggt
     5341 tgcgcacggc gtatgaccac tgcatggcgg cgcgctggaa ttgcttgcgg tcggcgcgca
     5401 gttcaaggtt cagcccctgc ggcgtgcgca cggtgatgct gacgcgtccg cccgtctccg
     5461 gcggcgcgcc cgcttcgacg aagccggcga gggacacagg tgcgacgacc gcgccggagc
     5521 cgccgcgcgc tggtggcgac tttttggaag ccggccgctt cttgctgctg ctgcgcgcct
     5581 gtgcgctcac actcttctta ccggccccgc ctttgcgcgc ggcggccccg cctttggccg
     5641 tcgctgctgc tccgcctttg gctgctcttg aggctttttt tgttgccatg aggtcgcgcg
     5701 cctttcttcg tgtctgaagg cagttgggtt agctcgcgcc gcgtcggacg gcgatgagga
     5761 cgcttcggct tttatcaaat tcgggcctcg gtggcaatgc ggacgcgcgc gcggacatcg
     5821 atttttcaga gcggcccgcc cggcgcgctc aacttgttat gcggatgcgg gccgagacgt
     5881 ttcacggcgg acgctcgccg agagaaaggg attaaggggc gctggttaca gatcaccccg
     5941 ccgtcttcaa aaaaaggcgg cggggtgtga aaggtgatcc gggtcgtgtg gcttcaggct
     6001 tgagtttcgg ccccgatgcc ctggtcggtc tgctggcgcg cgtggtagga ggagcgcgtg
     6061 agcgggcttg attcgacgtg cttgaagccc agagacatgc cgacctcgcg ccagcgggcg
     6121 aactcgtcgg gcgtgacgta gcgttcgacg gggaggcggc gcgcgtgcgg ctggagatac
     6181 tgcccgatgg tcataatgtc gcaggaggag gcgcgcaggt cgcgcatcag ctcgaccacc
     6241 tcgtcgaagg tctcgcccag tcccgccatg atgccgctct tggtgagcat cccccggcct
     6301 tcgcggtcgc ggcgctcggc cgcgcggcgc aacagcgtca tcgactgttc gtaacgggcg
     6361 tcgggccgga cgcgcttgta aagccgcgcg atggtctcgg tattgtggtt gagcacgtcg
     6421 ggcgcggcgt cgagcacggt gtcgagggcc gcctcgtcgc cgttgaaatc ggggatgagg
     6481 acttcgacgc ggcactctgg gttgagcgcg cgaatctgcg tgatggtctc ggcccagtgg
     6541 gctgcgccgc cgtccgcgag gtcgtcgcgg ttgacggagg tgataacggc gtgcgcgagg
     6601 tcgagagtct gcacggcctc ggcgacgtgg cgcggctccg ccgggtcgag tcggccgggg
     6661 gcgcccttgc cgacggcgca gaagccgcag tggcgcgtgc agatgtcgcc gccgatcata
     6721 aaggtggcgg tgcggtcggc ccagcactcg aagatgttcg ggcagcgcgc ctcctggcag
     6781 accgtgtgca ggttcagctt ctcgacgagg ccgagcagat aattatggtt cgccggatcg
     6841 ccgatccgca ccttcagcca ttcgggcttc ggagcacgcg gcggacgctg tggcgtcggg
     6901 gcgttactaa tgactcttaa ctcgcggctc aaaatttatc actccacatt gattttaaac
     6961 gtgctcgtat tttaacactc gcactacgcg cttgtctttg taggggcagg gcaagtccgc
     7021 ggcccgacgc ttacagggga attaacatgg atggacagga ttagcaggat gagttctccg
     7081 agtaggacag ggcagtagcc cgaccgtgag ggagggcgtg tggctcccgc gtcatgcgcc
     7141 ctccctcacg gtcgggctac tgccatgagc gcgcccccgt tgaaatctcc aatcagctct
     7201 ttcttcatcc tgctaatcct gtccatccat gttaattgct tccatcgttg cgcgggcagc
     7261 ggacttgtcc ctgccactac agtttatgac agcgagccgg cgacgacgcg cgccagcact
     7321 tcggggtcaa cgttgccgcc cgagagcacc acggcgggct ggagcacgtc ggcggggagc
     7381 ttgccgaaga ggacggccgc cgcggcgacg gcgcccgtcg gctcgacgag aatcttcatg
     7441 cggaagagca ggaagcgcat cgtctcgacg atctcgtcct cactcaccgt gagcacgtcc
     7501 tcggcgttgc gctgcacgac gggaaaggtg agcgcgccgg gcgactgtgt ccgcatgccg
     7561 tcggcgatgg tcggcggcgg cgggatacat acgcgctcgc ctttgcggaa cgattggcgc
     7621 gtgtcgtcgg agagttccga ctcgacgggg aagcagcgga ttttcgagtt caacgtcttg
     7681 accacgacgc tcgcgccggc gaagaggccg ccgccgccgc acggggtgag cagcgcgtcg
     7741 atgtcgggcg cgtcttcgag cagttcgagc gcgcaggttc cctgccccgc catgatgagg
     7801 aagtcgtcgt agggcgggac gagcaccaga ccttcgcgct ccgagatgtc gcgcgccaga
     7861 cgctcgcggt cgtcctgctg gcggtcgtag gtgacgacct ccgcgccgta gccgcgggtg
     7921 gccgcgagtt tcgggcgcgg ggcgtccgtc ggcatgacga tcacggcgcg cacgcccgct
     7981 tcgtgcgcgg cgagcgcgac cgactgcgcg tggttgcccg aggagaaggc gacgacgccg
     8041 cgccgccgct cgtcgtccgt cagtgcgcga atcttattag tcgcgccgcg catcttgaag
     8101 acgccggcgc gctgcaagtt ctcgcacttg aagaagaccg gacgaccggc ggcctcgttg
     8161 aaggcgcgcg aggtgaagac gggcgtgcgg tggacgtgcg gacggatgcg ctcttgcgcc
     8221 tcgcggatga gttcaaacgt caacatgcgt tgtgatcagc tcccctgata gcggcgcgcc
     8281 gtctgcgccc ggcacttctt ccggctccgc cccgaagacc tcagcgaagc gcgcaacgac
     8341 gcggtcttcc accgcgcgca gcgtcacgtc gcgcccgagt atgtcccgca tcgaggtgac
     8401 cggctcgccc tcgcaggcga ggatgagttt gaaatagtcg aggtcggtgt tgacgtttaa
     8461 ggcgaagccg tgtgtggtca cccagcggga gatgtggacg ccgatggcgg cgaccttgcc
     8521 ggcggcggtg tggacgccag tcagcccctc gatgcgaaac ccggagatgc ggaagtcgta
     8581 gaggacgcgc atcaagacct cctcgatgtc gcggacgtag cggtgtacgt cctcgcgctc
     8641 ggggctgagg ttgatgacgg gatagccgac cagttgtccg aggccgtggt aggtcagctt
     8701 ccccccgcgg ttcgactcgt ggacggcgac gccgcgcgcc tcaagctctt cttcggaggc
     8761 gaagatggac gcgcgggtcg cgcgccgccc gacggtcagg acgtgcgggt gctcgacgag
     8821 gagcagcgtg tcggcttgcg ccctcgcctt gaccgccgct tcggtctcct tctgaatccg
     8881 atacgccgcg tcgtattcga gccgcccgag gcggcggact ttgagcgtcc tctgtgccac
     8941 tggccctcac tctgccccgt cagaccgtcg ttgaaagctc tcgtctcatt ctagcagcgc
     9001 gcgcgcttct actgcgaacg gccgcccccg tcgcgccgcg ccgacacttg acgctcggcg
     9061 cgcgccggcc gtagtttgtt ggcagccgat ttcgtcaacc agagcatcac ttttaagacg
     9121 aggccggacg agtttgcccg acgcgcgaat ggcgcacgcg ggcggccgtc cccgccgtcc
     9181 gaaggaggaa agaattttga agcgccttta tccgctactg ctcgccattt ccctgttgct
     9241 gctcacgccg gtcattgccg gagcgcagtc gagaacccgc cgctcgtcaa cgccgcagag
     9301 acgccgcgcg cccgcctcca cgccggcgcg cgggagcaat gacgccgtcg tcctcggtcg
     9361 cctgaaggtg gccgagaaga tcaagatcat ttccgagttc ctgtacgtct acgggggcgt
     9421 cgctaatcag gtggcggcgg acgaggcgca ggcgcgccag agcggcgcga cggccgagta
     9481 tgcgagcgtg gccgaccgca gccgggcgtc tctgcgtaag agccttctgg ccgtgcgcga
     9541 cggactcgac cagctcgaag cggagttccg cacctcgccc gacctgcaac gctattacaa
     9601 ccggctcgcg ggcgtcgccg ccggcgcggc cgacgccgag gagctggccg cgtcgaacca
     9661 gttgaaaccc gccggccgca agctcgtgca ggtggtcagc cagctcgccg acgctttggc
     9721 ggcgatgaac taagaagaaa aagaggtcag aggttagagg tcagaggtca gtgaaagaca
     9781 gcattgtttt cactgacctc tgacctctaa cctctgacct ctttattttt gtcgctggac
     9841 gtagtagccc tgccgggcgc ggacgcggag gtcggcgcgc gcgggcgtcg tgacgcgaat
     9901 cttgaggaac ttgccgggcg ccgcgtcgct cgaagaatag tactggagca ggtattgcgc
     9961 gcgcagctcg gcggcgatgc ggcggaagat ggcgtcgagt tcggtgactt gcgcgggaag
    10021 gaacgaggag ccgccggtgg cctctgacaa ctgctgcatg ccgtactccg cgcgctggct
    10081 gatgacgttc agacgcacgc ccggcccgct cgggttgatc gaatagaaga ccgtgtcggc
    10141 ctgctgcacc tcacgcatca cgcgttgcaa ggcgcgccgg tgaacaccgg ccgtcccctc
    10201 gcccaccgtc tccaacttct cgcctgccgt cttctcgtct ccggctttgt tcgcgcggtc
    10261 gagcgccttg atggtggcgt cgcgcacgtc gatgctgaag ttgtcctcgc cgtcggagat
    10321 gacgaggacg acgcggcgat tcttgcccga ggcgttctgc gcgagatatt tggcggcggc
    10381 cgtcaccgtg tcgtagaagg ccgtgcccgt cgccttgtca gcggcgggga tggtcgtcag
    10441 cttccgcgcc gcgtcgtcgg cgccggcgag cggctgtatg aggttggagc gttcgccgat
    10501 ggtgaagacg gcggcgcgat ccgacggctt catcacttcc ttgaggaagc cggccgcggc
    10561 ctgcttcatg aagtcgaagt ttttgatggt gctgctcgac acgtcgaaga cgagagcgat
    10621 gtcgagcggc acggcttcgg cggcgccgac ctcggcgacc tcctgccggc ggccctcctc
    10681 ttcgagccgg aagtccgccg gctggagacc ctgcacgggg ttccccgccg cgtccacgac
    10741 cgaggtgggc acgacgacga ggttggagct gaccttaatg acttcgtcgt cttcaatcgc
    10801 gtccgccggc ttcgactcgc gcggggtcgg ctgcggagtg ggcgcgcgac gcggcggggg
    10861 cgggttgtcc caatcaggcg tcggactggg cgccgccgcc ggcggcagac gcggcacgac
    10921 gatgggcgtg ggctgctgcg tcgcccctgc cacgtcactc cggcgcgtct gactccgcgc
    10981 cgccgacgcc ggcgtgtgta agaccgacgc gagcgccacg cagacgactg caacgagcgt
    11041 caagaccgac agattcttcg cgccccggcg cgcttgcgac agcttcacga caggcccctc
    11101 cggttcgcct caaagaaaac gacaggtgac gaccgccgcg caaccgcgag gggggcgcgg
    11161 cggtcgtcac ctgttcggtc aacagcggaa tcggcagtcc ctagtcgggg ttctgcgtgg
    11221 acggcgtgtt catctgcgga gcgccctgaa gcagcccgcg gttgacgagc accttcacct
    11281 cgacgcgccg gttctgctcg cgccccgtgc gcgacgtgtt ctccgccacc tgctgcgact
    11341 caccgtagcc gaacggcgtg atgatgcgtc gcagcgggat gcggtggttc tcggccagat
    11401 agcgcacgac cgtgtcggcg cgcttctggc tcagccggcg gttgtggtcg gtcgcgccgt
    11461 cggcgctggc gtggcccgag acctcgatga cgtagccctt ggcgttgagg gcctgctgcg
    11521 cgatctggtc gagctgcgtc ttggcgtcgg gcgagagcac ggcgctgttg acgcggaagt
    11581 tcagggtcac gccctgctgg gcctcgtagt cgtcaagggc cgagatgcgc tcgttggtgg
    11641 cgcgcacgcc tgagacggca gcgtcggccg tctcctgcgc ggccttcgcg ccgccgcgcg
    11701 cggcgtt
//
LOCUS       DASVES010000514         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1672704, whole genome shotgun sequence.
ACCESSION   DASVES010000514 DASVES010000000
VERSION     DASVES010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672704"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VF240_21875"
     CDS             <1..1315
                     /locus_tag="VF240_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177924.1"
                     /translation="VLLLLLGLSAPSPVAGQTPATPAAPATGQAARPERIVGEVTATD
                     AAALTLTVKTDDGRTLPVTSDERTTYVRVAPGSTSLEGAAKITRADVGVGDRVLVLLR
                     AGTQGTAWPTLGLARQVIVTSRAELAASRESQREAARRRMLMGRIASIDATRREFTLT
                     TRGRGGPDAGVDTVTVAAGEGVRVLRFAPDSLRPEDARPATLADLRVGDQVRARGERS
                     ADGARFTPEEILAGSFVRAAGEITEVKAGEYTVKDELTGKLYTVVFSAKSTLRRVPRE
                     VAEEFARRREEQRERMGERRASGEPADRDARREARRAERAARGEGEGRRPDGEGGRRP
                     GGEGGRRREGGPGGNIQQMLEGLPAIAPTDLKKGDALLVTGTPDAAGTRVTAVAAYTG
                     EAEFLRRLNRIQERRGDVNTGLPGDVLGGGNVNREPTTNSPPPPR"
     gene            1523..>4426
                     /locus_tag="VF240_21880"
     CDS             1523..>4426
                     /locus_tag="VF240_21880"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7177925.1"
                     /translation="MLTSGKIFSRALARLPLAAVCVMLFAAAAGAQPSSGTLRGQVKD
                     EFGGVIVGATLTAADAAGVEKTATTDEEGRYSFAALPPGSYTVRVAAPGFAAYENVGV
                     EVAAGRADPLDITLAVAIEVEEVTVTAEADVSTDAENNAGAVVLRGTDLDALPDDPDD
                     LAEALQALAGPSVGASGEGEFFIDGFSGGRLPPKESIREIRINRNPFSAEYDRLGYGR
                     TEIFTKPGTDRFRGEAHFNFNDESLNSRSPFAPTRAAYQSRRYGGNFSGPLSPKRASF
                     FIDFERRETDDNEIVNAVVLDPQFAPVAFRQTIVTPDRRTEFGPRFDYQINQTNTLVA
                     RYSFERSRREDEGVGDFNLPERAYDVENTEHTLQLTETAIISQKLINETRFQYRGSRR
                     RQEGDAARPTLRVLDAFTGGGAQVGLSTNDTDRYELQNYSSWTLGNHSLKAGARLRHV
                     SIRDVSPQNFAGTYTFGGGLAPQLDAGGEIVRDAAGQPVLVNISSIERFRRTQVFLRD
                     GLTPAEIRARGGGATQFSISGGDPEATVSRTDFSPFIQDDWRVRPNLTVSLGLRYENQ
                     TNIDSHLNFAPRVAFAWSPGAGADARRQTTVIRGGLGVFYERFGENLTLQAVRQDGVS
                     RQQFLVTANTPGGTGVLDLFPTVPAFDALGGFAVAQSVRRVADDLQTPYTMQAALSVE
                     RQLPYRFTVSVNFIAARTLHVLRSRNTNAPFLLPSGERVRPTPGQGNLFLYESSGRFT
                     QRQLIVNVNNRYSRKFTLFANYVWNRAQSDTDGAGSFPANQYDLTGEYGRSAQDIRHR
                     LFLGGAINALPWGIRLNPFITINSGRPFNITTGQDLNGDTLFNDRPSLVSGDTCAART
                     VVSTSVVCTPYGTFNLRPSAGEPTIARNFAEGPGFFSVNLRASKTIGFGGAPAAAGGG
                     DAAGQGGRRGGGGGGRRGGGGGGGRGGGGRGGGGGGGTLGGDGGGGETASRYNL"
BASE COUNT          711 a         1602 c         1503 g          610 t
ORIGIN      
        1 tgtcttactg ctgctcctcg gcctgtcggc gccctccccc gtcgcggggc agacgccggc
       61 gacgcccgcg gcccccgcca cggggcaggc cgcgcggccc gagagaatcg tcggcgaggt
      121 gacggcgacg gacgcggccg cgctcacgct gaccgtgaag acggacgacg gccgcacgct
      181 cccggtcacc tccgacgaga ggacgaccta cgtgcgcgtc gcgcccggct ccacgtcgct
      241 cgaaggcgcg gcgaagatca cgcgcgccga cgtgggcgtg ggcgaccgcg tgctcgtcct
      301 cctgcgcgcg ggcacgcagg ggacggcgtg gccgacgttg ggactcgccc ggcaggtgat
      361 cgtgacgagc cgcgcggagc tggccgcgag ccgcgagagt cagcgcgagg ccgcgcgccg
      421 gcgcatgctc atgggccgca tcgcgtcgat tgacgcgacg cggcgtgagt tcacgttgac
      481 gacccgcggg cgcggcgggc cggacgcagg cgtggacacc gtcacggtcg cggccggcga
      541 gggcgtgcgc gtcctgcgct tcgcgcccga ctctctgcgg ccggaagacg cgcgccccgc
      601 caccctcgcc gatctgcgcg tgggcgatca ggtgcgcgcg cgcggcgagc gcagcgcaga
      661 cggcgcgcgc ttcacgcccg aggaaatcct cgccggctcg ttcgtgcgcg cggcgggcga
      721 gatcacggag gtgaaggcgg gagagtacac cgtcaaggac gagctgacgg gcaagctcta
      781 cacggtcgtc ttctcggcga agtcaacgct gcggcgcgtg ccgcgcgagg tggccgaaga
      841 gttcgcacga cggcgcgagg agcagcgcga gaggatgggc gagcgccgcg cttcgggcga
      901 gccggctgac cgcgacgcgc ggcgcgaggc gcgtcgggcg gagcgcgcgg cgcgcggcga
      961 gggcgaaggg aggcgtcccg atggtgaggg cggccggcgg ccgggtggcg aaggcggccg
     1021 gcggcgcgag ggcggccccg gcggcaacat ccagcagatg ctcgaagggc tgccggcaat
     1081 cgcgcccaca gatttgaaga agggcgacgc gctcctcgtc accggcacgc ccgacgcggc
     1141 gggcacacgc gtcacggccg tcgccgccta cacgggcgag gccgaattcc tccgtcgcct
     1201 caaccgcatt caggagcggc gcggcgacgt gaacacgggc ctgcccggcg acgtgctcgg
     1261 cggcggcaac gtcaaccgcg agccgacaac taattcgccg ccgccgccgc gctgacgctc
     1321 ggccgttttt gtaagggcag ggcaagtccg cggcccatgc tacggaggaa tcaacatgga
     1381 tggtcaggat tgtcaggatg aagaaaagat gatttcagat ttagagattt cagatttcaa
     1441 atcttcttct cctgttcatc ctacccatcc atgttaataa ttcgccggct attcgtggag
     1501 tgcgttcaca gggatgagga agatgctgac ttctggaaaa attttctcgc gggcactcgc
     1561 ccgtcttccg ctcgccgccg tctgcgtcat gctgttcgcc gcggccgccg gcgcgcagcc
     1621 gtcgtcgggc acgctgcgcg ggcaggtcaa agacgagttc ggcggcgtga tcgtcggcgc
     1681 gacgttgacg gcggccgacg ccgcgggcgt cgagaagacg gcgacgaccg acgaggaggg
     1741 gcgctacagt ttcgccgcgc tcccgccggg cagctacacc gtgcgcgtcg cggcgccggg
     1801 tttcgccgcc tacgagaacg tgggcgtgga ggtcgcggcc ggacgcgccg acccgttaga
     1861 catcacactc gccgtcgcca tcgaggttga agaagtgacc gtgacggccg aagcagacgt
     1921 gagcaccgac gccgagaaca acgcgggcgc cgtcgtcctg cgcggcacgg atttggacgc
     1981 cttgcccgac gacccggacg atttggccga ggcgcttcag gcactggccg ggccgtcggt
     2041 cggcgcgagc ggcgagggcg agttcttcat cgacggattc tcgggcggac gcctgccgcc
     2101 gaaggagtcg attcgcgaga tacgcatcaa ccgcaacccg ttctcggccg agtacgaccg
     2161 cctcggctac ggccgcaccg aaatcttcac caagccgggc accgaccgct tccgcggcga
     2221 agcccacttc aacttcaacg acgagagctt aaactcgcgc agccccttcg ccccgacgcg
     2281 cgccgcctac cagtcgcgtc gctacggcgg caacttctcc ggcccgcttt cccccaagcg
     2341 cgcctccttc ttcatcgact ttgagcggcg cgagacggac gacaacgaga tcgtcaacgc
     2401 cgtcgtcctc gacccgcagt tcgcgcccgt cgctttccgc cagaccatcg tcacgcccga
     2461 ccggcgcacg gagttcggcc cgcgcttcga ctaccagatc aaccagacca acacgctcgt
     2521 cgcccgctac agcttcgagc gcagccggcg cgaagacgaa ggcgtcggcg acttcaacct
     2581 gcccgagcgc gcctacgacg tggagaacac cgagcacacc ttacaactta ccgagacggc
     2641 catcatctcg cagaagctca tcaacgagac gcgcttccag tatcgcggca gccgccgccg
     2701 tcaggagggc gacgccgccc gtcccaccct tcgcgtgctc gacgccttca ccggcggcgg
     2761 cgcgcaggtc ggcctctcca ccaatgacac cgaccgctac gagcttcaga actacagctc
     2821 gtggactttg ggcaatcact cgctcaaggc cggcgcgcgc ctgcgccacg tgagcatcag
     2881 ggacgtgtcg ccacagaact tcgccggcac atacaccttc ggcggcggcc tcgccccgca
     2941 actcgacgca ggcggcgaaa tcgtccgcga cgcggcgggc cagcccgtcc tcgtcaacat
     3001 ctccagcatc gaacgcttcc gccgcacaca ggtttttctt cgcgacggac tcacgcccgc
     3061 cgagatacgc gcgcgcggcg gcggcgcgac gcagttctcg atcagcggcg gcgaccccga
     3121 ggccacagtc tcgcgcaccg acttcagccc gttcatacag gacgactggc gcgtgcgtcc
     3181 caacctcacc gtgagcctcg gactgcgcta cgagaaccag acgaacatcg acagccatct
     3241 gaacttcgcg ccgcgcgtgg cgtttgcgtg gtcgcccggc gcgggcgccg acgcgcgccg
     3301 gcagaccaca gttatccgcg gcggcctcgg cgtcttctac gaacgcttcg gggagaacct
     3361 gacgcttcag gccgtgcggc aggacggcgt cagccgccag cagttcctcg tcacggcgaa
     3421 cacgcccggc ggcacaggcg tgctcgacct cttccccacg gtgcccgcgt tcgacgcgct
     3481 gggcggcttc gccgtcgcgc agtcggtgcg ccgcgtcgcc gacgaccttc aaacgcctta
     3541 cacgatgcag gcggcgctga gcgtcgagcg gcagttgccc tatcgcttca ccgtctcggt
     3601 caacttcatc gcggcgcgca cgctccacgt cctgcgctcg cgcaacacga acgcgccctt
     3661 cctgctcccg tcgggcgagc gtgtgcgccc aacgcccgga cagggcaacc tcttcctcta
     3721 cgagtcgagc gggcgcttca cgcagcggca gttgatcgtc aacgtcaaca accgctacag
     3781 ccgcaagttc acgctcttcg ccaactacgt ctggaaccgc gcgcagagcg acacggacgg
     3841 ggcgggcagc ttccccgcca accagtacga cctcacgggc gagtacggcc gctccgcgca
     3901 ggacatccgc caccgcctct tcctcggcgg cgcgatcaac gccctgccgt ggggcatccg
     3961 cctcaacccc ttcatcacca tcaactcggg ccgccccttc aacatcacca cgggccagga
     4021 cttgaacggc gacacgctct tcaacgaccg cccctcgctc gtctccggcg acacctgcgc
     4081 cgcacgcacc gtcgtctcga cgagcgtcgt ctgcacgccc tacggcactt tcaacctgag
     4141 accgtcggcg ggcgagccga cgattgcgcg caacttcgcc gaggggccgg gcttcttcag
     4201 cgtcaacttg cgcgcgagta agaccatcgg cttcggcggc gcgccggcgg cggccggcgg
     4261 cggcgacgcg gccgggcaag gcggccgacg cggcggcggc ggcggtggtc gtcgcggcgg
     4321 aggtggcgga ggaggccgtg gcggcggtgg ccgtggcggc ggcggcgggg gcgggacgtt
     4381 aggcggcgac ggcggcggcg gcgagaccgc gagccgctac aacctg
//
LOCUS       DASVES010000515        19422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_485013, whole genome shotgun sequence.
ACCESSION   DASVES010000515 DASVES010000000
VERSION     DASVES010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19422
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485013"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VF240_21885"
     CDS             complement(<1..495)
                     /locus_tag="VF240_21885"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7177926.1"
                     /translation="MSRSIHIPSGRSVRARTAASDADVLARLREIVGAEHVLVDAERV
                     EPYAQDALKEKFPPEAVVLPGSTREVAEVLRLANERRFPVTARGGGVGYTGGAVPVEG
                     GIVLGTERMNRVREISADDLYVVTEPGVTTYALQQAVESHGLFYPPDPASYKDSYVGG
                     NIAEN"
     gene            complement(653..808)
                     /locus_tag="VF240_21890"
     CDS             complement(653..808)
                     /locus_tag="VF240_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177927.1"
                     /translation="MANDERKGKCSRLTCECSVPDNEKYCSEECEDAHKVNMMEIGCT
                     CHHPDCK"
     gene            complement(972..1661)
                     /locus_tag="VF240_21895"
     CDS             complement(972..1661)
                     /locus_tag="VF240_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177928.1"
                     /translation="MRVKAAVSLIFVACCCLANARAQDTSGDVRASEPRVALDAEAAA
                     YDIYGRAALAARLRTAALVGTPDAPGRNARIVIESRSQVFYTYASGWATFYGADNVRC
                     GEGMWKLEALAPGESAEVDTPGLRLTCTPATWRIVALNLLTRTTDAAKPADESAPPPA
                     ETTTPTQPGAATTPSMTPPTSSSAAPATARTLPPLEINVNGRVIPIQPGNPLEITVGA
                     ERVRIVLQPVP"
     gene            complement(1749..2984)
                     /locus_tag="VF240_21900"
     CDS             complement(1749..2984)
                     /locus_tag="VF240_21900"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX7177929.1"
                     /translation="MFRLNAALALLLLMTTFQQTNTERPRPRARDIGLHVGVLPPGRL
                     NAITDVSGVRVGHTTLVRGESVRTGVTAVVPHEGNLFQEKVPAAVFVGNGFGKLAGST
                     QVEELGEIETPILLTSTLNVPRAADALIDWMLQLPGNEDVRSINPVVGETNDGLLNDI
                     RGRHVGREEVFAALRSAKGGAVEEGAVGAGTGTIAFGFKGGIGTSSRVLPDSLGGYTV
                     GVLVQTNFGGVLTMNGAPVGRELGKYYLKDRLEAPTKARQGGESKGPAGGDRATDATT
                     ALQEDRADGSIIIVVATDAPLDARNLRRLAARAMLGLARTGSPSTQGSGDYVIAFSTA
                     PRNRIRAAEELRRVELLGNDAVSPLFLACVEATEEAIYNSMLRATTMTGHRAVRVEAL
                     PVERTVEILKKYNALAKEK"
     gene            complement(3074..3757)
                     /locus_tag="VF240_21905"
     CDS             complement(3074..3757)
                     /locus_tag="VF240_21905"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177930.1"
                     /translation="MAQVKRAAGVSTILDEASLARGVSRLVKRDGDLARVVEEYGRPA
                     LWAREPGFETLVLTILEQQVSLASAAATHARLLAAAGEVTPARLAGMSDAEVRAAGIT
                     RQKSVYLRDLARLIVEGGLDLRGLDKLDDDAARAELTKVKGIGAWTAEIYLLRALGRP
                     DAWPTGDLALAIAAERVKRLKSRPTTAELIVLAEAWRPWRSVAARILWQFYLHAPRKA
                     VSHQLSAVS"
     gene            complement(3757..5064)
                     /gene="larA"
                     /locus_tag="VF240_21910"
     CDS             complement(3757..5064)
                     /gene="larA"
                     /locus_tag="VF240_21910"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX7177931.1"
                     /translation="MPHVELKYGRGSTAFDFDAARFEVLAPAASDERPLTDAEIGAAI
                     DDPIDSQPLEEIVSPGESALVVVSDATRATGSAAIVHLLVRRLVQLGVAPYELKIIFA
                     TGLHRRVSSSERRELLTPFVVQRVRSLEHDPADESQLASLGTTERGTPIEINRALLDF
                     SHVILTGGVGFHYFAGFTGGRKSVCPGLASARTIAATHLLALDPERGGRRAGVGTGRL
                     DGNAVHEECERIAAEVAPSFLVNAVVDELGRPVRLYAGDWRSAHRHACADYADSHTLR
                     ISEKRPLVVVSAGGAPYDINLIQAHKALEMASHACEEGGHIILLAECAEGTGRPDFLK
                     WFEAADSRALERRLFEAYEVNGQTAWSLLTKTERFRIQLVSSLPEADVRSMGLHPART
                     FEEALAATDTKAPGYVMPRGAAFMPVVSPELADADTSLQPGIH"
     gene            complement(5223..6890)
                     /locus_tag="VF240_21915"
     CDS             complement(5223..6890)
                     /locus_tag="VF240_21915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177932.1"
                     /translation="MCVSLCFALSAFSQPTGALGQVDFPTSGSPAAQAHFLRGVAALH
                     SFWYEEALEEFQAATKVDPAFMMGYWGEAMAHNHPLWNEQDTEAARKVVAKIHDTGQL
                     TARESAYLGAVKALYGEGDRHARNLAYAAAMEKVYRAYPADLEAASFYALSLLGAVRP
                     VDKGYRLQMQAGAIALDVYGKNPNHPGAAHYIIHAFDDPEHAILALPAARRYAEIAPD
                     AHHARHMPSHIFIQLGMWAEAAASNESSWATSDAWVKRKKYSIERRDYHSLSWLEYVY
                     LQQGRTKEAEAQLALMQKSIADLGAPIRGYPYMAAAFIAETERWDLIEKHFGEPDAKS
                     ASLPIRKEAGADSCHAAAKPTKAKVIPASSGAPQAQRPLIKLLFGSYLRGMAAASSKN
                     PADAEKYSAELRALRKDFENSASYFTPEMVEIMALEVAGLARANAGKFDEAVELLRRA
                     TSLEESLRPPSGPPEMIKPSHELFGDVLLRAGRPAEAAAQFKTALLRQPNRPRSLLGL
                     ARAAAKSGDGKLAAQTYADYLRTQERADARAPELAEARDYLKQASVR"
     gene            complement(7075..7317)
                     /locus_tag="VF240_21920"
     CDS             complement(7075..7317)
                     /locus_tag="VF240_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177933.1"
                     /translation="MEINLLLVLDLREQAALQAALVTHGAPDHLVTLALTGACRIPSL
                     EEARKLRRWLSDARAAGNTDFNSLQAIERELVRFGV"
     gene            complement(7392..8363)
                     /locus_tag="VF240_21925"
     CDS             complement(7392..8363)
                     /locus_tag="VF240_21925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7177934.1"
                     /translation="MASTNGHDHKHGVGHGAHAHARADGSRRRLFLVLVLTALYMVAE
                     AVGGLVTGSLALLADAGHMLTDVAALALALTAAWFGSRPATPNKTFGYYRLEILAAFV
                     NGVALAVLSLYIFYEAYTRWSEPPVIARGGLLMVVAAGGLLINLFCAWLLHGDHEQDL
                     NVRGAWLHVMGDALGSVGAIAAGALVYFYGWQKADPLFSAVIALLIIYSSWRLIREAT
                     NVLLEGTPSHINLASVEGEIRATGGVEDVHDLHVWTITSGREALSAHVIHAPDISQHN
                     LLKELRAKLHDRFRIDHLTIQMETDDFEDEPFHFCHSGTACFRSAKE"
     gene            complement(8577..9392)
                     /locus_tag="VF240_21930"
     CDS             complement(8577..9392)
                     /locus_tag="VF240_21930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX7177935.1"
                     /translation="MIISCPNCSTRMQLDAAKVPARAFTVRCPKCQHIINAQPAAAPD
                     EGSAVAAGGDLPTTTRAQRETNGAPATDITLTPEEAISGEWRGEPSVEPSVAEAARLL
                     IELLGRGAAATTGPPADRADGARRRPAWDKHRALVCVSTSHRQEVARDLAREGYEVTQ
                     AGGTAQAIERMRGDKLDVLVLDNEFDMAEQGAAFLTREINSLRPSERRRLIVVHLSQS
                     GRTEDAHAAFLANANLIVNTGEVTELPRALERTVRDLNELYKNYNRALGAAEL"
     gene            complement(9482..10714)
                     /locus_tag="VF240_21935"
     CDS             complement(9482..10714)
                     /locus_tag="VF240_21935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7177936.1"
                     /translation="MSFFDLPPIIERMLLISDKISDLNFSVGQPPQVEINGRLTPVQP
                     LDTQKLTPYQTEVIAMTLMRGNPDAAQRLVHTGSTDLSYSLPSRTRFRVNIFQQRGTY
                     SIVMRVIPTEIPTLDTLTLPKQLSEVAELRNGIVLVTGPTGSGKSTTLAAIIDLVNER
                     HHYHIVTIEDPIEFLHTHKNSTINQREIGTDARDFPSSLRAALRQAPKVILIGEMRDY
                     ETTEIALEAAETGHLVLSTLHTIDAAKTIDRIIGLYPKNEEQVIRTRLAQTFRFIVSQ
                     RLIPRADGRGRQAAVEILKSTPRTREYIGEGESQGKSLLDAMHDGKLDGMQEFDFVIK
                     EMIEQQTITLEDGLAFATNPNNLVLALKGMSAAEEFASVADPHQQRPVARRRAGSLAP
                     PVSPPRPASPPSMLDMIE"
     gene            complement(10906..11544)
                     /gene="thiE"
                     /locus_tag="VF240_21940"
     CDS             complement(10906..11544)
                     /gene="thiE"
                     /locus_tag="VF240_21940"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX7177937.1"
                     /translation="MSDFKLPRVYPITDVSLSGLAHAEQVALLCEGGARLVQLREKRL
                     SPREFYRQTEEAVRVAHACGARVVINDRADIALAAGADGVHLGQDDLTPEAARRLLGR
                     AAIIGYSTHNVEQARAAAGMPVDYIAIGPVFDTASKERPHPVVGLDGVRRARAAVGSA
                     PLVAIGGITRHNARAVIDAGADCVAVIGALLSPAKPSEIAQKTRELLALLRD"
     gene            11830..13293
                     /locus_tag="VF240_21945"
     CDS             11830..13293
                     /locus_tag="VF240_21945"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7177938.1"
                     /translation="MKIKNITLLLLLSAALVPAHAHATSVRRTTRQAGRATVFQNVNV
                     IPMDRERVLKNQTVLVRDGRVVEMGDARKVRVPEGALRVDGRGKYLVPGLIDMHTHLF
                     SDDDFPDRLAGDELTLMLANGVTTIRLMIGTPEHLAMRAQVSRGEMPLAPTLYVASPQ
                     LTGHARYAESNGRHVTTPEQARQAVRDFKAAGYDFIKMTNFITPEVYDAATEAAREAG
                     IRVVGHVDTRVGVARALAAGQQIEHLDAYLESVLKDDSPIKTSVSDVGLFRKENWTSL
                     DHIDDNKVRAIARATARAGVYSTPTLTFFKITYGNGATDEEIRARPDYRFAPPHKRAL
                     WERVSKRLRDNQASEARRREYVRVRNLLVKEIHAAGGKIMTGSDTPEWFLLYGWTLHR
                     EMRSLVEAGLSNYAALEAATRNPAEYLKASGVFGTIAPGRRADLLLLDANPLDDIANT
                     ERRAGVMVRGRWLPDAELRKMLDDIAVRFQAVSGPEK"
     gene            complement(13462..13896)
                     /locus_tag="VF240_21950"
     CDS             complement(13462..13896)
                     /locus_tag="VF240_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177939.1"
                     /translation="MKKTSPARRQDPFPFVLQELLSVRPVVKRMFGFTHVYLGERLLC
                     SLRDSAKHPGTNGMWLYTTVEHLESLGSEFPELPRRYLWRSGKKGWVILASRLENFEE
                     YAYTACELILRGDRRVGRVTRGGRQLAEGEGDSGKWEGRGGA"
     gene            complement(14040..14423)
                     /locus_tag="VF240_21955"
     CDS             complement(14040..14423)
                     /locus_tag="VF240_21955"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX7177940.1"
                     /translation="MNKHLVFIYGTLRRGSPRAMSISFPNSKFIAEAQVSGQLYDLGA
                     YPGLLLEESNSSVTGEVYEVDDEILQRLDDFEASSNYLRKQVEISLGAHRQVCWTYEP
                     DPEFYSPRTLITSGDWIEYARTKTD"
     gene            complement(14553..16304)
                     /locus_tag="VF240_21960"
     CDS             complement(14553..16304)
                     /locus_tag="VF240_21960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7177941.1"
                     /translation="MHTAQTDEPTAQTDKPTSQKPLRLWPGVVVVVLQLLGLFVIPLV
                     VPEAAIIGMLGGAVGALAILVWWVFFSRARWPERLGVIVLIIAALAATRFILHPSIAG
                     AGMGFLFYVYGLISVSIALVVGVVIGRRLSAGPRLAVLAAAILIGCGAWALVRTGGIS
                     GDGDSDLAWRWSKTPEEQLLAQAGNEPAARQSAPAAAVTGADWPGFRGPERDGVVRGV
                     RIKTDWSASPPQELWRRPVGPGWSSFAVRGDRFYTQEQRGEDEAVTCYNVNTGQPVWT
                     HRDKARFYESNGGAGPRGTPTLSDGRVYTLGGTGILNVLNADDGAVVWSRNAASDTQT
                     KVPGWGFSGSPLVIDDMVVVAAAGTLAAYDRATGEPRWVGPKSGSGYSSPHRVTNGGV
                     TQIVLMNGGGAISVAPSDGKLLWQHPLPTGGRIVQPALTADGELLLSVGEGSDLYRVA
                     VAQRDGGWTAEERWTSEGLKPYFSDFVVHRGHAYGFDAGKIACINVEDGKGKWEGGSY
                     GSGQLVLLAEQDVLLLVSEQGELALVGATPDKFTELARFPAIKGKTWNHPALAGDVLL
                     VRNSEEMAAFRLSPLDR"
     gene            complement(16340..17404)
                     /locus_tag="VF240_21965"
     CDS             complement(16340..17404)
                     /locus_tag="VF240_21965"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7177942.1"
                     /translation="MDDSLPVRLREVDLGHRGYPLEGPRDIEELHAVSGVRQAQLAHD
                     LSEDAGQLSGPLERMIRDHRMKTTRFALLVMSGLMVWSCQKGTASAALQIPHPDDASK
                     QVEYFVEAPAGKGPWPTIVLLHGHQEGNRPGGKDFVDWGVLKQMSLRGYLAVAVSQPG
                     YGNSAGPADFSGAFTQHAVAGVIAKLRADGQVSPDKLLIEGISRGALTAGLVAAHDPS
                     VSGLVLISGVYDLPAYLADAAASAEKSMVVQSLSAEAGTSADALNARSVLRFASSIKA
                     PSLILNGAKDDRTDPGQARALAEQINRSGGNAKAIIYPDFGHQIPVEVRNKDIDPFID
                     RVLGNWRPESEVKPRPETMK"
     gene            17756..18007
                     /locus_tag="VF240_21970"
     CDS             17756..18007
                     /locus_tag="VF240_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177943.1"
                     /translation="MSDYQFASEYRRENDLTLRIAEAAKEWRLAALDYDKTLEEWNAE
                     ADPQKKDALWEQLQQYEMIRDGAQAKVAMLADRYISEAI"
     gene            complement(18070..>19422)
                     /locus_tag="VF240_21975"
     CDS             complement(18070..>19422)
                     /locus_tag="VF240_21975"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7177944.1"
                     /translation="WVLLLDPATGKTTVLANVHDDAWVGGPGSFTLGWLADNRTVYFQ
                     SERDGFAHLHAVSSDGGQARQLTTGPFEVSDVRLSRDKTKFFFTSSEGSPFERHLYSM
                     PAAGGERTRITTMPGNNQTEISPDETRLAVVRSYSNSPPELYLQANRPAPPAGAKGVV
                     PIRVTTSPTDEWLTHNWIDPPIVSFKARDGATVHARLYKPAGSPAGGPAVIFVHGAGY
                     LQNVHRWWSSYFREYMFHHLLAERGFTVLDIDYRGSAGYGRDWRTGIHGHMGGKDLTD
                     HVDAARYLAAEHGVDPKRVGIYGGSYGGFITLMAMFTEADVFAAGAALRPVTDWAHYN
                     HPYTANILNTPQGDLEAYKRSSPIYFAEGLRGALLICHGMVDTNVHFQDSVRLAQRLI
                     ELRKENWELAAYPVEDHSFERASSWADEYKRILKLFSEHLKGDAGTPPARPPGTARKR
                     "
BASE COUNT         3278 a         6163 c         6561 g         3420 t
ORIGIN      
        1 gttctcggcg atgttgccgc cgacgtagga gtccttgtag gaggccgggt cgggcgggta
       61 gaagaggccg tgcgactcga ccgcctgttg cagcgcgtag gtcgtcacgc ccggctcggt
      121 cacgacgtag aggtcgtccg ccgaaatctc gcggacacgg ttcatgcgct ccgtgcccaa
      181 cacgatgccg ccctcgacgg gcacggcccc gcccgtgtag ccgacgccgc cgccgcgcgc
      241 cgtgacgggg aagcgccgct cattagcgag ccgcagcacc tccgcgacct cgcgcgtcga
      301 gccgggcagc accacggcct caggcggaaa cttttccttc agcgcatcct gcgcgtaagg
      361 ctcgacccgc tccgcatcga cgagcacatg ctccgcgccg acgatctccc gcaggcgcgc
      421 cagcacatca gcgtccgagg cggccgtgcg cgcgcgcacg ctacggcccg aaggtatgtg
      481 aatagagcgg ctcatgcttt tgtttgcgaa atcgcgcact actgtaacgc gcggatgagc
      541 gacacatcaa acgcggctga acgcgccata agtcggaagg gcggcggcgc cgtagtgtcg
      601 cgccgccgcc cttccttatg tttacatctt ccctgccgac ggccgtcgcc ggctatttgc
      661 agtcggggtg gtgacaggtg cagccgatct ccatcatgtt gaccttgtgg gcgtcctcgc
      721 actcctcgct gcaatacttt tcgttgtcgg ggacggaaca ctcacaggtc agccgcgaac
      781 acttgccctt cctctcatcg tttgccatgg gcgctctacc tcctcgcgtg gcgttcgagc
      841 actatggggg ccgtgcgtgt gcgctaagtt tcgacccgac ccggcgccca cgtcaagtgg
      901 ggaaagggta aggggtaaag gggaaaggca ggtctgaccc ttacccttta ccttttcccc
      961 ttaccctccg tttacgggac gggttggagg acgatgcgga cgcgctcggc gccgacggtg
     1021 atctccagag ggttgccggg ctggatgggg atgacgcgcc cgttgacgtt gatttcgaga
     1081 ggcggcagcg tgcgcgctgt ggctggcgcg gcggacgagg acgttggtgg cgtcatcgaa
     1141 ggcgttgtgg ccgcgccggg ctgtgtcggc gtggtggtct cggcgggcgg cggcgcgctc
     1201 tcgtcggcgg gcttggcggc gtcggtcgtg cgcgtcagga ggtttaaggc gacgatgcgc
     1261 caggtcgccg gcgtgcaggt caggcgcagg cccggcgtgt ccacctcggc cgactcgccc
     1321 ggcgcgagtg cttcgagctt ccacatgccc tcgccgcaac ggacgttgtc cgcgccgtag
     1381 aaggtggccc agcccgaggc gtaggtgtag aagacctgac tccggctctc gatgacgatg
     1441 cgggcgttgc gaccgggagc gtcgggtgtg ccgacgaggg cggcggtgcg caggcgcgcg
     1501 gcgagggcgg cgcggccgta gatgtcgtag gcggcggcct cggcgtcgag cgccacgcgc
     1561 ggctcggagg cgcgcacgtc gcccgacgtg tcctgtgcgc gcgcgttcgc gaggcagcag
     1621 cacgcgacga agatgagact cacagcagcc ttcactctca taagccctcc agacgaaaac
     1681 cgtttccgac ccctagctta caacgcagac gctgaaagcc caacgccttt ccccgtcatc
     1741 tctccccgtc acttctcttt cgcgagcgcg ttgtacttct tcagaatctc gaccgttcgc
     1801 tccacgggca gcgcctcgac gcggacggcg cggtggccag tcatcgtcgt cgcacgcagc
     1861 atcgagttgt agatggcctc ctcggtcgcc tcgacgcagg cgaggaagag cggcgagacg
     1921 gcgtcgttgc cgagcagttc gacgcgacgc aattcttcgg cagcgcggat gcgattcctg
     1981 ggcgcggtgc tgaaggcgat gacgtaatcg ccgcttccct gcgtggaggg cgagccggtg
     2041 cgcgcaaggc cgagcatggc gcgggcggcg aggcggcgca ggttgcgcgc gtcgagcggc
     2101 gcgtccgtgg cgacgacgat gatgatcgag ccgtcggcac ggtcttcctg tagcgccgtc
     2161 gtcgcgtcgg tcgcgcggtc gccgcccgcc ggccccttcg actcgccgcc ctgccgcgcc
     2221 ttagtcggcg cttcgagtcg gtctttcaga taatacttgc ccagctcgcg gcccacgggc
     2281 gcgccgttca tcgtcagcac gccgccgaag tttgtctgca cgagcacgcc gaccgtgtag
     2341 ccgcccaagc tgtcgggcag gacgcgcgac gacgtgccga tgccgccctt gaagccgaag
     2401 gcgatggtgc ccgtgcccgc gccgaccgcg ccctcctcga ccgccccgcc cttcgccgag
     2461 cgcaaggccg cgaagacctc ctcgcgcccg acgtgccggc cgcgaatgtc gttgagcagc
     2521 ccgtcgttcg tctcgccgac gacggggttg atcgagcgca cgtcctcgtt gccgggaagc
     2581 tgtagcatcc agtcgatcag agcgtcggcc gcgcgcggga cgttgagcgt gctcgtcaaa
     2641 agtatcggcg tctcgatctc gcccagctct tcgacctgtg tggagccggc gagcttgccg
     2701 aagccgttgc cgacgaagac ggccgccggc accttctcct gaaacagatt tccctcgtgc
     2761 ggcacgacgg ccgtcacgcc tgtgcgcacg ctctcgccgc gcacgagcgt cgtgtggccg
     2821 acgcgcacgc ccgaaacgtc cgtgatggcg ttgagcctgc ccggcggcag cacgccgacg
     2881 tggaggccga tgtcgcgcgc gcgcgggcgc ggcctctcgg tgtttgtctg ttggaaggtt
     2941 gtcatcagga gcaggagcgc gagtgcggcg ttgagtctga acatatgtag aagcgggttg
     3001 cgaccccgtg tgcgcgcgtc agatgttgta tctcagtatg gccgcgaagc tgaccactga
     3061 caaagaacat cgcttagctg accgctgaaa gctgatggct gacggctttc ctcggcgcgt
     3121 gcaaatagaa ctgccacagg atgcgcgccg ccacggagcg ccacggccgc cacgcttccg
     3181 ccaacacaat caactccgct gtcgtcgggc gcgatttcag acgcttgacg cgctcggccg
     3241 cgatggccag cgcgaggtcg cccgtcggcc acgcgtcggg tcgtcccagc gcccgcaaaa
     3301 gataaatctc cgccgtccac gccccgatgc ctttgacctt cgtcagctcc gcgcgcgccg
     3361 cgtcgtcgtc caacttgtcg agaccgcgca gatcgagtcc gccttcgacg atcagccgcg
     3421 cgaggtctcg caggtacacg gacttctgcc gggtgatgcc ggcggccctg acctccgcgt
     3481 cgctcatccc ggccagccgc gccggagtca cttccccggc cgccgcgagc agccgcgcgt
     3541 gtgtggccgc cgccgaggcg agcgacacct gctgctcaag gatggtgagc acgagcgtct
     3601 cgaagcccgg ctcgcgcgcc cacagcgccg gccgcccata ctcctccacg acgcgcgcca
     3661 aatcgccgtc gcgcttcaca agccgggaga cgccgcgcgc cagcgacgcc tcatcgagaa
     3721 tcgtagacac gcccgccgcg cgcttaacct gcgccatcaa tgtatccccg gctgtagaga
     3781 tgtgtcggcg tccgcaagtt cagggctgac caccggcatg aacgcggcgc cgcgcggcat
     3841 gacgtagccg ggcgctttcg tgtccgtggc ggcgagcgcc tcttcgaacg tgcgcgccgg
     3901 gtgcagaccc atgcttctca cgtccgcttc ggggagcgat gagacgagtt ggatgcggaa
     3961 gcgctcggtc ttcgtgagta acgaccacgc ggtctgaccg ttgacctcgt aggcttcgaa
     4021 cagccggcgc tccagcgcgc gcgagtcggc ggcctcgaac cacttgagga agtcggggcg
     4081 gcccgtgccc tcggcacact cggccagcag gatgatgtgg ccgccctcct cgcaggcatg
     4141 cgaggccatc tccagagcct tgtgcgcttg aatgagattg atgtcgtagg gcgcgccgcc
     4201 cgcggagacg acgacgagcg ggcgcttctc tgaaatcctg agcgtgtgcg agtcggcgta
     4261 gtcggcgcag gcgtggcgat gggcggagcg ccaatcgccg gcgtagagac gcacgggccg
     4321 ccccaactca tcgacgacgg cgttgacgag aaacgacggc gcgacctcgg cggcgatgcg
     4381 ctcgcactcc tcgtggacgg cgttgccgtc gagccgtccc gtcccgacgc ccgcgcgccg
     4441 gccgccgcgc tcggggtcga gcgcgagcag gtgcgtggcc gcgatggtgc gcgccgaggc
     4501 gagtccgggg cagacgcttt tgcgcccgcc cgtgaagccg gcgaaatagt ggaagccgac
     4561 gccgcccgtg aggatgacgt gcgagaagtc gagcagggcg cgattgatct cgatgggcgt
     4621 gccgcgctcg gtcgtgccga ggctcgcgag ttgcgattcg tccgcggggt cgtgttcgag
     4681 tgagcggacg cgctggacga cgaagggcgt caacagctcg cgccgctcgg acgagctgac
     4741 gcggcggtgc agccccgtgg cgaaaatgat cttcaactcg tagggcgcca ctcccaactg
     4801 gacgagccgg cgcacgagca ggtggacgat tgcggcgctt cccgtagcgc gtgtggcatc
     4861 cgagacgacg acgagcgcgc tctcgccggg gctgacgatc tcttcgagcg gctgcgagtc
     4921 gatggggtca tctatggcgg cgccgatctc cgcgtccgtg agcgggcgct cgtctgacgc
     4981 ggcgggcgca agcacctcga agcgcgcggc gtcgaagtcg aaggcggtgg agccgcggcc
     5041 gtatttgagt tcaacgtggg gcataaaggc agggagtcag gagtcagaag tcagaagtca
     5101 ggagtcagaa taaagataga cggtgacagc ttctatatgc agactccatg ccgattcggt
     5161 catctgtaaa ccgaatttta gacttcggag tttccaacca cttcgacgct caagcaggtc
     5221 atctaacgca cgctcgcttg tttcaagtag tcgcgcgcct cggcgagttc gggggcacgc
     5281 gcgtcggccc gctcctgtgt gcgtagataa tcggcgtaag tttgcgcggc gagtttcccg
     5341 tcaccgcttt tggcggcggc gcgcgccagc ccgagcagcg agcgcggccg gttcggctgg
     5401 cgcaaaagcg cggtcttgaa ctgcgccgcc gcttccgccg ggcgacccgc gcgcagcaac
     5461 acgtcaccaa acaactcatg tgacggcttg atcatctcag gcggccccga aggcggtcgc
     5521 agcgattctt ccagactcgt cgcgcgccgc aaaagttcga cggcctcgtc aaatttgccc
     5581 gcgttcgccc tcgccagccc cgcaacttcc aacgccatga tctcaaccat ctccggggtg
     5641 aaataactcg cggagttttc aaaatcctta cgcagagcgc gaagctcggc cgaatacttc
     5701 tccgcgtccg ccgggttctt cgatgaggcc gccgccatgc cgcgcaggta actgccgaac
     5761 aaaagtttga tcaacggtcg ttgcgcctgc ggcgcgccgc ttgacgccgg aatcactttc
     5821 gccttcgtcg gcttcgcggc ggcgtggcat gagtctgccc ccgcttcctt ccttatcggg
     5881 agcgacgcgg atttcgcgtc gggttcgccg aagtgttttt cgatcaaatc ccaacgctcg
     5941 gtttcggcga tgaaggcggc ggccatgtaa ggatagccgc gaatcggtgc gcccaaatcg
     6001 gcgattgatt tctgcatgag ggcgagttgc gcttcggcct cttttgtgcg cccctgctgc
     6061 aagtagacgt attcgagcca actgagactg tgataatcac gacgctcgat ggaatatttt
     6121 ttgcgcttga cccaggcgtc ggatgtcgcc cacgaagact cgttcgaggc ggcggcctcc
     6181 gcccacatgc cgagctgaat aaagatgtgt gaaggcatgt ggcgcgcgtg atgcgcgtcg
     6241 ggcgcgatct cggcgtagcg gcgcgcggcc ggaagcgcga ggatggcgtg ttcgggatcg
     6301 tcgaaggcgt gaatgatgta atgcgccgcg ccgggatgat tcggattctt gccgtaaacg
     6361 tcgagcgcga tggctccggc ctgcatctgc aaacggtagc ccttatcgac ggggcgcacg
     6421 gcgccgagca acgagagcgc gtagaaggag gcggcttcga ggtcggccgg ataggctcta
     6481 taaactttct ccatggcggc ggcgtaggcg agattgcgcg cgtgcctgtc gccttcgccg
     6541 tagagagcct tcacggcgcc gaggtaggcc gactcgcgcg cggtgagttg cccggtgtcg
     6601 tgaattttcg cgacgacttt gcgcgcggct tccgtgtcct gctcgttcca gagcgggtgg
     6661 ttgtgcgcca tggcctcgcc ccagtagccc atcataaaag ccgggtcaac tttcgtcgcc
     6721 gcttgaaact cttccaaagc ctcttcatac cagaacgagt gcaacgccgc gaccccgcgc
     6781 aggaaatgcg cctgcgccgc cggcgagccg gaagtcggga aatcaacttg cccgagcgcg
     6841 cccgtcggct gcgagaaggc ggataaagcg aaacacaggg aaacgcacaa tgttaaaaat
     6901 gtccgttgcg aagcacgacg aaacgacatg actttacctc gactttcttg cagcagggtg
     6961 aatgccttgt agtgttgcca ccacgctcat cacgatttca ggtcaaatat aaatacacgg
     7021 ccgcggcctt ttttgttccg actcctgact cctgactcct gacttcttga ttgttcagac
     7081 cccgaagcgc accagctcgc gctcgatggc ctgaagggag ttgaagtcgg tgttgccggc
     7141 ggcgcgggcg tcggagagcc agcggcggag cttgcgcgcc tcttcgagcg aggggatgcg
     7201 gcaggcgccc gtcagcgcca gcgtcaccag atggtcgggc gcgccgtgcg tgacgagcgc
     7261 cgcctgaagc gccgcctgct cgcgcaaatc gaggacgagg aggagattga tttccatagc
     7321 tcaagttcaa agttcaaagt tcaaagttca aaggggccgt cgcctttgaa ctttgaactt
     7381 tgaactttga actattcctt cgccgagcgg aagcaggccg tgcccgagtg gcagaagtgg
     7441 aacggctcgt cctcgaaatc gtcggtctcc atctggatgg tcaggtggtc gatgcggaag
     7501 cggtcgtgaa gtttggcgcg cagctcctta agcaggttgt gctgcgaaat gtcgggcgcg
     7561 tggatgacgt gcgcggagag cgcttcgcgg cccgaggtga tcgtccagac gtgcaggtcg
     7621 tgtacgtcct cgacgccgcc cgtcgcgcgt atctcaccct cgaccgaggc gaggttgatg
     7681 tgcgacggcg tgccttcgag gagcacgttc gtcgcctcgc ggatgagcct ccatgagctg
     7741 tagatgatga gcagggcgat gacggcgcta aagagcgggt cggccttctg ccagccgtag
     7801 aaatagacga gcgcgccggc ggcgatggcg cccaccgagc cgagggcgtc gcccatgacg
     7861 tgcagccacg cgccgcggac gttcaagtcc tgctcgtggt cgccgtgcag cagccacgcg
     7921 cagaacaggt tgatgaggag gccgccggcc gcgacgacca tcagcagccc gccgcgcgcg
     7981 atgacgggcg gctccgacca acgcgtgtac gcctcgtaga agatgtagag cgagaggacg
     8041 gcgagggcga cgccgttgac gaaggcggcg aggatttcga ggcggtagta gccgaaggtc
     8101 ttgttggggg tggcggggcg cgagccgaac cacgcggccg tgagcgcgag tgcgagcgcg
     8161 gccacgtctg tgagcatgtg gccggcgtcg gcgaggagcg cgagcgagcc ggtgacgagg
     8221 ccgcccacgg cctcggcgac catgtagagg gccgtgagca cgagcacgag gaagagtcgg
     8281 cggcggctgc cgtcggcgcg cgcgtgcgcg tgggcgccgt gcccgacgcc gtgtttgtgg
     8341 tcgtgtccgt tcgtcgaggc catcgtcttc gagagttctt ccaggctcga aagctgtccg
     8401 ggcaggggtg taagagtagc agatttccgc cggctcggtg tagtgcgccc cggcgctcat
     8461 gggccgtgca aaagaagacc gcccccgacg acgcgcaagc gtcgggggcg gtctcggctc
     8521 gcctctttcg tggcggaccc ggccgacgcc tcagcgagag cgtccccgct acggcctcac
     8581 agctcggcgg cgccgagcgc ccggttgtaa ttcttgtaca gctcgtttaa gtcgcggacg
     8641 gtgcgttcga gcgcgcgcgg cagctccgtc acctctcccg tgttgacgat gaggttggcg
     8701 ttggcgagga aggcggcgtg cgcgtcctcc gtgcgacccg actgcgagag gtggacgacg
     8761 atgaggcggc ggcgctcgga cggccgcagg gagttaatct cgcgcgtgag aaaggcggcg
     8821 ccctgctcgg ccatgtcgaa ctcgttgtcg agcaccagca cgtccagctt gtcgccgcgc
     8881 atgcgctcga tggcctgcgc ggtgccgccg gcctgtgtca cttcatagcc ctcccgcgcg
     8941 aggtctcgtg cgacctcctg ccggtgagag gtgctgacgc agacgagcgc gcggtgcttg
     9001 tcccacgcgg ggcgacggcg cgcgccatcg gcgcggtcag cgggggggcc ggtggtcgcc
     9061 gcagcgccgc gaccgagcag ctcgatgagg agtcgcgcgg cctccgccac ggacggctct
     9121 acagacggct cgccgcgcca ctcgccgctg atagcctcct cgggcgtcag ggtgatgtct
     9181 gtggccgggg cgccgttggt ttcgcgttgc gcgcgcgtgg tcgtcggcag gtcgccgccc
     9241 gcggccacgg cgctcccctc gtcgggcgcg gcggcgggct gcgcgttgat gatgtgctga
     9301 cacttcgggc agcgcacggt gaaggcgcgc gccggcacct tcgcggcgtc gagctgcatg
     9361 cgcgtcgagc agttcgggca ggagataatc ataacttttg ttaggagtca ggagtcagga
     9421 gtcaggagtc agaatgaaga aggtagtgca tgctttcatt ctgactcctg actccctgct
     9481 cttattcaat catgtccaac atcgagggtg gggaggcggg gcgcgggggg gacacggggg
     9541 gcgcgaggct gccggcgcgt ctcctggcga ccggccgctg ctggtggggg tcggcgacgc
     9601 tggcgaactc ttcggcggcg ctcatcccct tgagtgcgag cacgaggttg ttggggttgg
     9661 tggcaaacgc gagaccgtct tcgagggtga tggtctgctg ctcgatcatc tccttgatga
     9721 cgaagtcgaa ctcctgcatg ccgtcgagct tgccgtcgtg catcgcgtcg agcagggatt
     9781 tgccctgcga ctcgccctcg ccgatgtact cgcgcgtgcg cggcgtggac ttcagaatct
     9841 cgaccgccgc ttgccggccg cgcccgtcgg cgcgcgggat gaggcgctgc gagacgatga
     9901 agcggaaggt ctgggcgagg cgcgtgcgga tgacctgctc ctcgtttttc gggtagaggc
     9961 cgatgatgcg gtcgatggtc ttggcggcgt cgatggtgtg cagggtcgag agcacgaggt
    10021 gcccggtctc ggccgcctcc aaagcgatct ccgtggtctc gtagtcgcgc atctcgccga
    10081 tgagaatcac cttcggcgcc tgacgcagag cggcccgcaa ggaggagggg aagtcgcgcg
    10141 cgtccgtgcc gatctcgcgc tggttgatgg tcgagttctt gtgcgtgtgg agaaactcga
    10201 tggggtcttc gatggtgacg atgtggtagt ggtggcgctc gttgacgagg tcgatgatgg
    10261 cggccagagt cgttgacttt ccggagccgg tcgggccggt gacgaggacg atgccgttgc
    10321 gcagctcggc gacttcggag agctgtttgg ggagggtgag ggtgtcgagc gtcggaatct
    10381 cggtggggat gacgcgcatc acgatggagt aggtgccgcg ctgctggaag atgttgacgc
    10441 gaaagcgcgt gcgcgagggc aaactgtagc tgaggtcggt cgagccggtg tggacgagtc
    10501 gctgcgcggc gtcggggttg ccgcgcatca gcgtcatggc gatgacctcg gtctggtagg
    10561 gcgtcagctt ctgcgtgtcg agcggctgga cgggcgtcag ccggccgttg atctcgacct
    10621 gcgggggctg gccgacggag aagttgaggt cgctgatctt gtcagagatc agcagcatgc
    10681 gctcgatgat gggcggcaga tcgaagaaac tcatgccagg ctttaggctc ccgaagtatg
    10741 aaggaagaaa ggagagttgt tggcggcggg cgcggcggcg gttcgacctc cggcgcatgt
    10801 acgctgcccg aaggctcgac cattgtcgcg caacgactgt taaaaatcaa gtttaaactt
    10861 ttaaccgccg ctattttaac acttgccggc agagctgagg aggtcttagt cgcgcagcag
    10921 ggcaaggagt tcgcgtgtct tttgggcgat ttcggagggc ttcgcgggtg agaggagggc
    10981 gccgatgacg gccacgcagt cggcgccggc gtcgatgacg gcgcgggcgt tgtggcgcgt
    11041 gatgccgccg atggcgacaa gaggcgccga gccgacggcg gcgcgcgcgc ggcgcacgcc
    11101 gtcgaggccg accaccggat ggggccgctc cttcgaggcg gtgtcgaaga cggggccgat
    11161 ggcgatgtaa tcgaccggca tcccggcggc cgcgcgcgcc tgttcgacgt tgtgtgtcga
    11221 atagccgatg atggccgcgc gcccgagcag gcggcgggcg gcctccgggg tgaggtcgtc
    11281 ctggccgaga tgcacgccgt cggcgccggc ggcaagcgcg atgtcggcgc ggtcgttgat
    11341 gacgacacgc gcgccgcatg cgtgcgccac gcgcacggct tcctccgtct gccggtaaaa
    11401 ctcacgcggc gacagacgct tctcccgcaa ctggacaagc cgggcgccgc cctcacacaa
    11461 gagcgccacc tgctcggcgt gcgcgaggcc cgacagcgac acgtccgtga tggggtagac
    11521 cctcggcagt ttgaaatcac tcacgtcttc aaccctgcgg cgccccctct gcgtcctctg
    11581 cgcctgcggc ggtgaatcta ctccgcttcc actcaagtga gtactacgca agtatccggg
    11641 ttaacggatt attttcaaac tcacgccctg tctcacccgt cacacccgcc cccggcctgt
    11701 ccttctcgcc cccgtttctt ctcggctggt cacaaactca ttcagagtta gccggggcgc
    11761 ggcctaagat gtagcgccgc ggccggatgg cccgcgcgct ctgacattct ttcttcgagg
    11821 aagcccgaaa tgaagattaa aaatatcacg ctcctgctcc tgctgtcggc ggcgctcgtt
    11881 cccgcgcacg cgcacgccac gagcgtgcgg cgcaccacgc gtcaggcggg gcgggcgacc
    11941 gtgttccaga acgtcaacgt catcccgatg gatcgcgagc gtgtgctgaa gaatcagacg
    12001 gtgttggtgc gtgacggccg tgtcgtcgaa atgggcgacg cgcggaaggt gcgcgtcccc
    12061 gaaggcgccc tgcgcgtcga tggtcgcggc aaatatctcg tgccgggcct catcgacatg
    12121 cacacgcacc tcttctcgga cgacgacttc cccgaccgtc tggccggcga cgagttgacg
    12181 ctgatgctgg cgaacggcgt gacgacgatt cgcctgatga tcggcacgcc ggagcacctc
    12241 gcgatgcgcg cgcaggtgtc gcggggcgag atgccgctcg cgccgacgct ctacgtggcc
    12301 agcccgcagt tgaccggcca cgcgcgctac gccgagtcga acgggcggca cgtgacgacg
    12361 ccggagcagg cccgacaggc agtgcgcgac ttcaaggccg ccggctacga cttcatcaag
    12421 atgaccaact tcatcacgcc cgaggtctac gacgcggcca cggaggcggc ccgcgaggcc
    12481 ggcatccgcg tcgtcggcca cgtggacacg cgcgtcgggg tcgcgcgcgc gctcgcggcc
    12541 ggacagcaga tcgaacacct cgacgcgtac cttgagtccg tcctcaaaga cgactcgccg
    12601 attaagacct ccgtgtccga cgtgggactg ttccgcaagg agaactggac aagtctcgac
    12661 cacatcgacg acaacaaggt gcgcgccatc gcgcgggcga cggcgcgcgc gggcgtctac
    12721 tccacgccga cgctcacctt cttcaagatc acctacggca acggcgcgac cgacgaggag
    12781 atacgcgcgc gccccgacta ccgcttcgcc ccgccgcata agcgcgcgct ttgggagcgc
    12841 gtgagcaagc gactgcggga caatcaggct tcggaggcgc ggcggcgcga gtacgtgcgc
    12901 gtgcgcaacc ttctggtcaa agagattcac gcagcgggcg ggaagattat gaccggctca
    12961 gacacgcccg agtggttcct gctctatggc tggacgctgc accgcgaaat gcgcagcctc
    13021 gtcgaggccg gcctgagtaa ctacgccgcg ctcgaagccg cgacgcgcaa cccggccgag
    13081 tacctgaagg cgtcgggcgt cttcggcaca attgcgccgg ggcgtcgcgc cgatctcctg
    13141 ctgctcgacg ccaacccgct cgacgacatc gccaacaccg agcgccgcgc cggcgtgatg
    13201 gtgcgcggcc gctggctgcc cgacgcggaa ctgcgaaaga tgctcgacga catcgccgtc
    13261 aggttccagg ccgtctccgg gccagagaaa taagacggcg tcaccacgcc tttgagtcga
    13321 gtgtgcggca ggtcgggttc accgcggagg cgcaaaggac gcggaggggg cgcggaggct
    13381 cgaatcaatc ctctgcgcgg actccgcgcc ccctgcgcct cggcggtgaa tctatttccc
    13441 ttccactcaa gtgaacatcg ctcaagcgcc gccgcgcccc tcccacttgc ctgagtcccc
    13501 ttccccctcc gccaactgcc ggccgccccg cgtcaccctg ccgacccttc ggtcgcctct
    13561 caggatcagc tcgcacgccg tgtacgcgta ctcctcgaag ttttccagcc ttgaggcgag
    13621 gatcacccag cccttcttcc ccgagcgcca caggtaacgc cgcggcaact cggggaactc
    13681 gctgcccaga ctctccaggt gctcgaccgt cgtgtacaac cacatgccgt tagtgcccgg
    13741 atgcttggcg ctgtcgcgga gtgagcagag aagccgctcg ccgaggtaga cgtgtgtgaa
    13801 gccgaacatc cgtttgacga cggggcgaac gctcagcagc tcttgcagca cgaacgggaa
    13861 ggggtcttgc cttctggcgg gcgaagtctt cttcatcacg agccttgggt gggctgcctt
    13921 cctgcccggt gaagggcgcg gcgtgcccgc cccgtcggtt tggcggatgc tacagcaaca
    13981 gcgtgacggc gcggcggtga cgaacctgct gcgcggcagt taaagtctgt gcgaacaggt
    14041 cagtctgttt tcgttctggc atattcaatc caatcacctg atgtaattaa tgtacgcggt
    14101 gaataaaatt cagggtcagg ctcataagtc caacagacct gtcggtgggc gccgagagag
    14161 atttcaacct gcttccgcaa ataattgctt gacgcttcaa aatcatccaa cctttgcaga
    14221 atctcatcgt caacctcata cacttcgccg gtaaccgatg agttcgattc ctcaagcagc
    14281 aagccgggat atgcacccag atcatagagc tggccgctca cttgagcttc ggcaataaat
    14341 tttgaattgg ggaaactgat agacattgcg cgcggactgc cgcgtctcaa cgtaccgtaa
    14401 ataaatacga gatgcttatt cactgagttt tactttagta agagccacac cgtcagcccg
    14461 atagcgccac ccgccgcctg aagttgcgtc catttaaaca ggtgcaacga gttgttgggc
    14521 ggcgcccgcg atggccacga aggcacgccc cgtcagcggt ccagcgggga cagtcggaac
    14581 gcggccatct cctcgctgtt gcgaaccagc aggacatcgc cggccagcgc cgggtgattc
    14641 catgtcttgc ccttgatcgc cgggaaccgc gccagctccg tgaacttgtc gggagtcgcc
    14701 ccgaccagcg ccagctcacc ttgctccgac accagcagca gcacgtcctg ttcggccagc
    14761 aggacgagct ggccggagcc gtagcttccg ccctcccact tgcccttgcc gtcctcgacg
    14821 ttgatgcagg cgatcttgcc ggcgtcgaac ccgtaggcgt ggccacgatg cacgacgaag
    14881 tcgctgaagt acggtttcag tccctcggat gtccagcgct cttcggccgt ccatccgccg
    14941 tcccgctgcg cgaccgcgac gcggtacagg tcgctgccct cgccgacgct gagcaacagc
    15001 tcgccgtccg cggtcagggc gggctgcacg atgcggccgc ccgtcggcag cgggtgttgc
    15061 cagagcagct tgccatcgga cggcgcgacg ctgatggccc cgcctccgtt catcagcacg
    15121 atctgcgtga ccccgccgtt ggtcacgcgg tggggcgagc tgtagccact gccgcttttc
    15181 gggccgaccc agcgcggctc gccggtggcg cggtcgtaag cggcgagcgt gccggcggcg
    15241 gcgacgacga ccatgtcatc gatcaccagc ggtgaacctg agaagcccca gcccggcacc
    15301 ttcgtctgcg tgtcggacgc cgcgttgcgc gaccagacga cggccccgtc gtcggcattg
    15361 agcacgttca ggattccggt tccgccgagc gtgtagaccc ggccgtcgct gagggtcggc
    15421 gtcccgcgcg ggccggcgcc gccgttcgac tcatagaatc gagccttgtc gcggtgagtc
    15481 cacaccggct ggccggtgtt cacgttgtag caggtgacgg cttcgtcctc accgcgctgt
    15541 tcctgtgtgt agaagcggtc gccgcgaacc gcgaaggacg accagcccgg cccgacgggg
    15601 cgacgccaca gctcttgcgg cggcgacgcc gaccagtcgg tcttgattcg cacgccgcgg
    15661 acgacgccgt cgcgttcggg tccgcgaaag ccgggccagt cggctcccgt taccgctgcc
    15721 gccggagctg actggcgcgc tgccggctcg ttgccggctt gagccagcag ttgctcctca
    15781 ggggtcttcg accaacgcca cgctaaatct gaatcgccgt cgccgctgat gccgccggtg
    15841 cggacgagcg cccacgcccc gcacccgatc aggatggccg cggcgaggac agcgagccgc
    15901 ggcccggcag agaggcgacg gccgatcacc accccgacga cgagcgcgat gctcacggag
    15961 atgaggccgt agacgtagaa caaaaacccc atccccgccc ccgcaatcga tgggtggagg
    16021 atgaaacgtg tcgcggccaa cgcggcgatg atcaggacga tgacgcccag acgctcgggc
    16081 caacgcgccc ggctgaagaa gacccaccac acgagtatcg ccagcgcgcc gacggccccg
    16141 cccaacatcc cgatgatcgc ggcctcgggc acgacgaggg gaataacgaa cagccccagc
    16201 agttgcagca ccacgacgac tacgccgggc cacagccgga gcggcttctg agatgtcggt
    16261 ttgtcggtct gtgcggtcgg ttcgtcggtc tgtgcggtgt gcataccaga ccaatacgca
    16321 cctcaacagg ccgtttgttt catttcatgg tttccgggcg gggctttact tctgactcag
    16381 gacgccagtt tcccagcacc cggtcgatga aaggatcgat atctttattc cgcacctcga
    16441 ctggaatctg gtggccgaag tcggggtaga taatcgcttt tgcgttaccg ccgctacggt
    16501 tgatttgttc tgccagagcg cgcgcctgac cggggtcggt gcggtcatcc ttcgctccgt
    16561 tcaggattag tgatggcgct ttgatgctac tcgcgaaccg caggacggaa cgtgcgttca
    16621 aggcatcagc gctggttccc gcttctgcgc ttagtgactg caccaccatc gatttctccg
    16681 cgcttgccgc tgcgtccgcc aaatacgccg gcaagtcgta gacgcccgat atcagcacta
    16741 accctgaaac ggaagggtcg tgggcggcaa ccagaccagc cgtcagagcg ccgcggctga
    16801 taccttcaat gaggagtttg tccggtgaca cctgaccatc cgcacggagc ttcgcaatga
    16861 cgccagcgac cgcgtgctgt gtaaatgctc cgctgaagtc ggccggcccc gccgaattcc
    16921 cgtaaccggg ttgcgaaaca gcgactgcca gataaccccg cagagacatc tgcttgagca
    16981 caccccaatc gacaaagtct ttgcctcccg gccgattccc ctcttgatgt ccgtgcagca
    17041 gaactattgt cggccaaggc cccttgcccg caggcgcttc gacaaagtat tcaacctgtt
    17101 ttgaggcgtc gtccggatgc ggaatctgta gggcggccga tgccgtgcct ttctggcatg
    17161 accagaccat cagcccggac atgacgagga gagcgaaccg cgtggtcttc atacgatgat
    17221 cccttatcat cctctcaaga ggcccactca actgcccggc gtcttccgat aggtcgtgtg
    17281 ccaactgcgc ttgccgcacc cctgacaccg catgtaattc ctcgatgtcc cgtgggcctt
    17341 ccaacggata cccccgatgt cccagatcga cctctcgtag ccgcaccggc agcgaatcat
    17401 ccattcacgc gaatctgcct ccatgctcct ggcccagctc gccgggaaga tcgccacgaa
    17461 gaacttctgg attaagctca tacgagattc acctccgccg gggcatgcga ccctcacgcc
    17521 tttaagcggc ccaacccgcg ccgagcgcag gcattcaatt tacatcacag gaaaaatcat
    17581 gaaaagtatg ctatcgagga cggcgggttg aataagttgt taggcgtggt attccagggt
    17641 ggtaatctga cgcccgaact taagccgcgc gggcgaatca agctgggctg aaggcggcgc
    17701 catacgcgag cctgatttga cggggtgtta cattgcctct gagaggaaca atttaatgtc
    17761 agattatcaa ttcgcttcgg agtacagacg ggaaaacgac ctgactctgc gaatcgcgga
    17821 ggcggccaaa gagtggcggc tcgccgcgct cgattacgac aagacgctcg aagaatggaa
    17881 cgccgaagct gacccgcaaa agaaggacgc cctgtgggag caattacaac agtacgagat
    17941 gattcgagac ggggcgcaag caaaggtggc gatgctcgcg gaccgctaca tcagcgaagc
    18001 aatctaacgc ccgacatcac ccgccgcgcg tccgaccccc tctttggccg ttcactaccc
    18061 tcatgtcagc taacgcttgc gcgccgtgcc gggcggtctg gctggtggtg tgccagcgtc
    18121 acccttaagg tgttccgaaa acaatttgag gatgcgcttg tactcgtcgg cccaggagga
    18181 ggcgcgctcg aaggagtggt cttcgacggg gtaggcggcc agttcccagt tctccttgcg
    18241 tagctcgatg aggcgttggg cgaggcggac ggagtcctgg aagtggacgt tggtgtccac
    18301 catgccgtga cagatgagga gggcgccgcg caagccttcg gcgaagtaga tgggtgagct
    18361 gcgcttgtaa gcctcgaggt ctccctgcgg cgtattgagg atattggcgg tgtaggggtg
    18421 gttgtagtgc gcccagtcgg tgacgggccg gagggcggcg ccggcggcga acacgtcggc
    18481 ttcggtgaac atggccatca gcgtgatgaa gccgccatag gagccgccgt agatgccgac
    18541 gcgtttcgga tcgacgccgt gttcggcggc gagatagcgc gcggcgtcaa cgtggtcggt
    18601 caaatccttg ccgcccatgt ggccgtggat gcccgtgcgc cagtcgcgtc cgtagccggc
    18661 cgagccgcgg tagtcgatgt cgagcacggt gaagccgcgc tcggcgagga ggtggtggaa
    18721 catgtactcg cggaaatagc tcgaccacca gcgatgcacg ttctgtagat agcccgcgcc
    18781 gtggacgaag atgacggcgg ggccgccagc aggggagccg gccggcttgt agagacgcgc
    18841 gtggacggtc gcgccgtcgc gcgccttgaa gctgacgatg ggcgggtcga tccagttgtg
    18901 cgtgagccac tcgtcggtgg gcgaggtcgt cacgcgaatg gggacgactc ctttcgcgcc
    18961 ggccggtggg gcggggcggt tggcttgcag gtaaagctcg ggcgggctgt tgctgtagga
    19021 gcggacgacg gcgaggcgcg tctcgtcggg ggagatttcc gtctggttgt tgccgggcat
    19081 cgtggtgatg cgcgtgcgct cgccgcccgc cgcgggcatc gagtagaggt gacgctcgaa
    19141 ggggctgccc tcgctcgaag tgaagaagaa cttcgtcttg tcgcgggaga gacgcacgtc
    19201 ggagacctcg aacggccccg tggtgagttg gcgcgcttga ccgccgtcgc tggagacggc
    19261 gtggaggtgc gcgaagccgt cgcgctcgga ctggaagtag acggtgcggt tgtcggcgag
    19321 ccagccgagg gtgaaggagc cggggccgcc gacccatgcg tcgtcgtgga cgttggcgag
    19381 cacagtggtc ttgcccgtgg cggggtcgag caggagcacc ca
//
LOCUS       DASVES010000516        22612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1088917, whole genome shotgun sequence.
ACCESSION   DASVES010000516 DASVES010000000
VERSION     DASVES010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22612
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088917"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /gene="thiD"
                     /locus_tag="VF240_21980"
     CDS             <1..751
                     /gene="thiD"
                     /locus_tag="VF240_21980"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX7177945.1"
                     /translation="TSLTYQNTTGVFGAAHQSATAVRAQVLPVVEDFRVAAAKTGMLP
                     TREVIREVARLFRETDLPAPVVDPVVRSTSGYDLIDDEALAALRDELLPLARLLTPNI
                     PEAERLCGMRIENRAGMLRAAQSLRGMGARAVLVKGGHPFVADGGDAAQEAADLLLEE
                     DERALILSAPRVASTSTHGTGCTLAAAIAACLARGMELKEATATAKRYVTEAIRRAPG
                     LGRGHGPVNHAVVPRVGAWGALGSEERYDEV"
     gene            738..2147
                     /locus_tag="VF240_21985"
     CDS             738..2147
                     /locus_tag="VF240_21985"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7177946.1"
                     /translation="MMKSEKFFDLRTALRAATRHALAACLLTAAAATLARAQGDLTGG
                     IGQFAAVQPKKEKQAKQARPKVVKPAASASVPRVNAAHEQLLRAAAAGNLQQTQAALA
                     AGGKVNYKGKTGLTPLMLAADAGSKEVVALLLARGADLAARSAEGCTSLIYAVAGAHT
                     ELTQTLLSKGADVNARCGDGLTALTLAAEDGDATAVATLIARGADVNAASADGRTPLM
                     FAASSGHLEIAQALLAKSADVKARQRDGRTPLHFAAANGHGTVAAALIARGAEVDAKP
                     DDGWTPLLWASWYGHLSIVKDLVERGANISARDRDGDTPLMKAATQGHTAIAQLLIAR
                     GAEVDARGNGQGTALIFAASRGVPELIALLFEKKADVNARNDAGLTPLILAASHGRVS
                     VVQDLLQRGANPNAATAKGYTALMAAANKGLAQTVSSLIAGGADLNLKYEDGRTALSF
                     ATQNGQDKVIQILKQAGAK"
     gene            2221..2937
                     /locus_tag="VF240_21990"
     CDS             2221..2937
                     /locus_tag="VF240_21990"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX7177947.1"
                     /translation="MSSQPTAPTAFGPRGRRRALSLLALVVLTAAVSSCAGLSEQGAS
                     TGTTNAPNGQAVANNQPAPGTLRANASATVPGAPATSPPQVAPTPGRMALPDAVKLAE
                     INALDGAPIRFSEFGGKVVVLNLWATWCGPCRLEIPHLVELNKEYAGRVEFVGLTTED
                     RAASEPLVREFVREYKMDYRVGFSGPTVALALMQNRTSIPQVFIIRDGYIHYRFIGFN
                     PQISPPRLREGIEATLKAGA"
     gene            complement(3050..3547)
                     /locus_tag="VF240_21995"
     CDS             complement(3050..3547)
                     /locus_tag="VF240_21995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7177948.1"
                     /translation="MPNRIARIETNMGTIRFELLEEDAPKTTENFRLLAERGAFDGVI
                     FHRVIKGFMIQGGDPTGTGRGGASAWGGKFNDEINASTPLYRGPYERGTVAMANAGAN
                     TNGSQFFIMHSDYPLPPSYTKFGRVVAGQDVVDKIATAPTAGGDRPLNEVKMEKVTIE
                     EGPGE"
     gene            complement(3619..4518)
                     /locus_tag="VF240_22000"
     CDS             complement(3619..4518)
                     /locus_tag="VF240_22000"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX7177949.1"
                     /translation="MSIRLIALDLDGTLLDSRGNLPERNRRAIERARERGVRIALVTG
                     RRFRDARPLALELGLDVPVISHNGALTKHARTLETVAALLLPKEAALAVARAGRARGE
                     DALVSDDHHGLGVLVYDHLSAGNVALAKYVAWARRIHGPEADEAVRLVPSLEEYLDHD
                     PVHVTFSGACDRMSALSESLAGELGGAVKLLLTLYQKMDFALLDVLHPLASKGAGVAA
                     AAAEQGLARADVMAVGDNLNDLEMLEYAGTGVLMGNAEQSLLTRKHVHRTATNDEGGV
                     AEAIERFVLTEHGESGVRSQESE"
     gene            4886..6052
                     /locus_tag="VF240_22005"
     CDS             4886..6052
                     /locus_tag="VF240_22005"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7177950.1"
                     /translation="MNVTHLECAACGLRHEARRLHNLCRECARPLLVRYDLRSASETL
                     TRESLKDRRADLWRYREVLPVEHEENIVSLGEGWTPLLRADRLGRSLGLRHLFIKDES
                     QNPTQSFKARGMSAAVSMAKELGVTKTAVPSAGNAAGALAAYAARAGMEAHIFMPRDT
                     PRANVVECEQTGASVTLMDGLITDCGAEVGRRKDAEGWFDVSTLKEPYRVEGKKTLGY
                     ELAEQLDWELPDLIIYPTGGGTGLIGMWKAFDEMEQMGWIGGKRPRMVSVQAAGCAPI
                     VRAFEAGVRHAEEFQDAATVASGLRVPRAIGDFLILDALRASNGRAIAVSDEELLAAV
                     GEIGAAEGLFVAPEGAACLPALRRLMADGAVGEDERVVLFNTGAGVKYLESFGG"
     gene            6306..7664
                     /locus_tag="VF240_22010"
     CDS             6306..7664
                     /locus_tag="VF240_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177951.1"
                     /translation="MMWRRLGPVLLSAVFLSVCAAAQTGRSAMKGSGASQPPKPAAAG
                     EAADSTRLDALRAEGFEALYNLDYERARTKFKEVARLFPDHPAGPQFLAATVWLKTLN
                     ESRRLQASLYNTEGFYAKAKEEADPKVLAEFRELTAQAKTLAQARLKRDPKDAEALYF
                     YGATEGLKAAFAAAVERSFYSAWRDGQEAADRHRELLKLDPNYHDAGITVGMYDYIAA
                     TLPLPFRIGGSLVGLRGSKKRGLQTLERVAREGRWAQDDAKVLLIALYKREGRFGEAL
                     TFARELSAKYQRNYIFKMETADALVSLAAAARASDPAAAGKSETEALAIFEALLQQKP
                     SKGEPARPLDLIHFSYGQALAVAGQHERAAKEYLLGAAAPGAERGLATMSRLRAAQSL
                     DQAGRREEAQAQYREVLTRPNVYDSHEEAQRGLKEPFKPRAGGAEDSGGRAGQKTAAG
                     SE"
     gene            7742..9070
                     /locus_tag="VF240_22015"
     CDS             7742..9070
                     /locus_tag="VF240_22015"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7177952.1"
                     /translation="MSALQRASAEMVGARARPRPVSVTSARPRRLLLCLDGVPHEVIT
                     AAKGRGMFDSFGEPSRLLSPFPTMTNVALALMLRATPPAGYESLYFDHGERRLSGGVR
                     KYIGRRTPDKMPSSYMDELDYQEPLAFEFLIYVATERVWRADLRRFRERFRATSPARD
                     FFGFLKATDGLLHKCGPRSLSVALRSLDQILRDIQEACGSETEIVLFSDHGMNLEENR
                     RVHLQEGLRRSGFEVTADLRGGRGRARVSTPAFGLCSYAALYCGDETDPAEVAAATGT
                     LEGVDFSVRRDGEGTGVLVEGARGRAHIRRRERAAGVASYSYECADGDPLGLSPIVSA
                     LAQEGLLDAQGFAPDATWLERTAEHIYPDAPANLHGSLYAPRVRHTADLLLSLHDGYF
                     FGDTAFSHMIRLRATHGNALRSSSNAFLMSTHRTFAPHVRAADVRPLLRD"
     gene            complement(9096..9551)
                     /locus_tag="VF240_22020"
     CDS             complement(9096..9551)
                     /locus_tag="VF240_22020"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7177953.1"
                     /translation="MMRRIVLSVALVLSMSVAAAAQSDAEREAVRQAVLDYVEGVYEI
                     APARIERSIHPELAKRGFYVKKGETSYSPHTMTFQQLVDLAKSYNKDGHVKKDAPKEI
                     VIYDISDQTASIKLTAIWGIDYMHLAKYEGRWKIINVLWQTPPKRDESR"
     gene            9731..10564
                     /locus_tag="VF240_22025"
     CDS             9731..10564
                     /locus_tag="VF240_22025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7177954.1"
                     /translation="MTTDDTADAEHAPGGAGAAVETFELTRRFGEFAAVDRLDLNVAR
                     GQFFGFLGPNGAGKSTTIKMLTGLLAPTSGRIRVLGLDLEDHPLDVKRRIGVVPEDLN
                     LFERLTGAEMLTFTGRMYGLARADILERARELLELMELDSEPRKLVIEYSHGMKKKLS
                     LACALIHRPEILFLDEPFEGVDAIASRTLKDLLARLTSRGLTVFLTSHVLEIVERLCT
                     DIAIISGGRLVASGTLDELRRGISVEGDGHAASMTLEDYFIRVVGGARTSGDEVLQWL
                     G"
     gene            complement(10842..14144)
                     /locus_tag="VF240_22030"
     CDS             complement(10842..14144)
                     /locus_tag="VF240_22030"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7177955.1"
                     /translation="MPTPTLRLFALAAALVIIAGAAVVRAQTAVTSPLPLRGASLSRT
                     HVAFEYADDIWTVGREGGDARRLTDYPGKDSLPFYSPDSSRIAFARHNPVSGPLAWDV
                     YVMPAAGGEAVRLTYHPELDAPAGWTPDGKSVLFYTLRDRMSSISARLYTVPADGGPE
                     EPLPLPSGWAGSFSPDQTRLAYTPLLDPLQVFQFRNYRGGAMSKIWLARLSDSVVEEI
                     PRGEANDLNPIWVGERVYFLSDRAGPYNLFVYDTRTKAVSQLTRFEKYDVRWASHGGD
                     SILLVHDGALHLFDPQTNQTRRVDVRVAGDFPQTKPRTIDAGQSADNILPSPDGKQLL
                     LGARGEILTARVSDGEVTNLTRTPAAAERGAVWSPDGRWLAYFSDESGELELHVRTLA
                     PSSGNSAAGRGATSGGAARVVDSAVVGAVRRFTIDRPSSIYAELLWSPDSSKLAFTDA
                     LLTLRYLDLATGAVRRVDASTHTDGGTFMHPAWSPDGRWLAYAKYGVNRVRTVTLFSL
                     ETGKTYAVSDQRIDAQQPTFDRGGEYLYFIGSNQTGLVESQSMSGFPFRSQVTRSLYA
                     VVLNRESPSPLAPRAGDDDVARVRTIHIDPEGIAERVLPLSIGVGNPGRLMAGTHGTL
                     FVVEGVVLHKFRAGTQGTEKFVEGAGNYRVTPDGKHLLLRRQGRWAMAPADAAPPKPD
                     DGRFTPKSMELTIDPRAEWRLLYEEAWRRMRVYFYDPNLHGINAGELKSHYAAYLPNV
                     VTRDGLNEVFKETFSHLSTSHMGIWGGDSPAPQGLEAETTGLLGADYETDSGRYRIRR
                     VLRGDPVLGVTGPLAQHGLGAGVKAGEYLLAVESVEVRAGDNLYRHFVNRASKPLKIS
                     VGARADGVGAREVRVVPAASEYALRQIEWVRENRRRAEELSGGRLAYVYMTNTSDDGY
                     NAFNRDFYAQLDRQGLILDARFNQGGRAADYVIDTLRRAPLYRAALRAGEDIRIPTGI
                     IDGPKVVLTNESAGSGGDTLPWIAKRTGVATVVGTRTSGAGVGATDHNLLDGGQVRVP
                     DWGWYDHAAGTWLVENRGTTPDVILENMPADWRRGRDPQLERAVAIALEQLKKKGPPT
                     PPHRPKYPVYRQ"
     gene            14512..16002
                     /locus_tag="VF240_22035"
     CDS             14512..16002
                     /locus_tag="VF240_22035"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177956.1"
                     /translation="MYHPPEAIRHEIPETRWPTREEAAGALLFQPVGVGPVRLESRTW
                     VPAMVPWRATDDGFVTRENIDWYARFAEGRPGAIVVEATGVRDIPSGPLLRIGHDRFL
                     PGLRELVETVGRASGGHTRLFIQIIDFLSVKRRPERAKFFERFLVVEDRHRRGLAAAT
                     ADAGWLERDEPELRAFLAASADDLVRRVLDEREWEALAFGYRESVTDMHLPHVRELPQ
                     VLPGIFAAAARRARAAGFDGVELHYAHAYTMASFLSRRNDRQDGYGATPEGRVRLPLE
                     VYRAVRAEVGADYCVGVRFLGDEVIAGGSRIDDAVYYAAEFARAGFDFLSVSKGGKFE
                     DAKQPKVGHAVYPYTGESGYECMPTVISDARGPFGRNVPLVRAVKRAVSETGRATPVV
                     AAGGISTFEQAEAILRRGDADIIGTARQSLADPDWFLKTKLGRGDEVRRCTYTNYCEA
                     LDQMHRQVTCKLWDRVGLDEAGVSLSMDGRRRLLPPRWDGDNAEAS"
     gene            complement(16040..17014)
                     /locus_tag="VF240_22040"
     CDS             complement(16040..17014)
                     /locus_tag="VF240_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177957.1"
                     /translation="MKQASSTGARRIPAGASQTGPAARVAIALSLFTLFTFGAACADT
                     AAAGGPHAAAGASQSTFSGTWTAELRDDKAGERLQFDIRTDAQRGSGNFSGSDYSLAD
                     FQGLTREQISARTDTPVSFRLVREAGTVECKGSFRGGRGTGTWLLNPSQSFRSAMSSR
                     GYGDLTDREMLTSTLLNLTVSFVDDLKSVGFDRLSFDDAIKALIFKVTPQYVAEMKSL
                     GFENLGLEELVKARIFKVDAEFARQVQEMGFAGQSLEELVKLRIFKVTPDFLEELKAE
                     GFSQVSIEDAVKLKIFNIDRDFIRRAKASNADLNIEDLVQLRIHGRLR"
     gene            complement(17140..18276)
                     /locus_tag="VF240_22045"
     CDS             complement(17140..18276)
                     /locus_tag="VF240_22045"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7177958.1"
                     /translation="MRINVIGGGPAGLYFALLMKKRWPSHEITVFERNAPDSTFGWGV
                     VFSGKTLSNLRAADEETYRSITESFETWDNVDVLKGDDKVTIRGNHFSGIARLRLLNI
                     LQERCDGLGVSLRYREEVADVDELAGSSDLVVGADGVNSRVRERFAEEFQPALALGGN
                     KYVWYGTRQLFHGLTLTFRESGAGLFAAHSYKFDRATSTFIVECDEKTWARAGFAEMS
                     DEAARSYLGEVFDSDLGGHELLSNNSRWVNFLLVRNERWFHQNVVLLGDALHTAHFSI
                     GSGTKLALEDAIALFERFAAGRGVREALAEFERTRKPVIEEYQRAAHDSRLWFERAAD
                     YMHLPPLQFAYSLMTRSGKVDFESLRRRDPAFVEAYERAVSPAN"
     gene            complement(18286..18819)
                     /locus_tag="VF240_22050"
     CDS             complement(18286..18819)
                     /locus_tag="VF240_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177959.1"
                     /translation="MLDELGISYVVVGSFASSMRGLYRATGDIDIMADIKAEHVKPLV
                     AALQEDFYVDDLAVRRAVARHGSFNAIHFDSVFKIDVFIPPADDFSVQQLARRQLETI
                     APDVNQKIYVATAEDTVLSKLKWYRAGGEGSDMQWRDVLGVIGTQGESLDVEYLRIWA
                     AKLNLNDLLERVLSEAS"
     gene            complement(18862..19137)
                     /locus_tag="VF240_22055"
     CDS             complement(18862..19137)
                     /locus_tag="VF240_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177960.1"
                     /translation="MKPLSPDTTPEAQQMMFKLMRRTPVWKRLALTCELIQATRLLML
                     ADLRRRFPLADEEELRRRLIAELLPRDEVIRAYGFDPGNVSHLAHGR"
     gene            complement(19188..20366)
                     /locus_tag="VF240_22060"
     CDS             complement(19188..20366)
                     /locus_tag="VF240_22060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7177961.1"
                     /translation="MPDLFYEETPFFTDEHRRLAASVAQFVATDIERRAAGDEEERDG
                     DAHFRELLGLLGQTGLLSYSVPQLDNTPAVDVRSLCLIREALAYSSPLADLAFVMQGL
                     GTYALSLAGAEHIRDFWLSRASTGKSIAAFALTEPEAGSDVSAIQTTAQRDGDGYRID
                     GRKHFISNAGLADFYTVFARTGTRADGRPEISAFVVSARLAGFRVVERTRLIAAHPIG
                     VVEFEALRVPEEDRVGAEGDGHRLAMQTMNTFRASVGAAACGMARRALDESLRHAKSR
                     RQFGQPLASFQLVQAKLAEMATDLDAARLLVYRAAHLRDEGTATDSALARAASQAKLF
                     ATEAAQRIIDEAVQIHGAQGLTRGHVVERLYRDVRALRIYEGTSEIQKLVIANQLLKD
                     "
     gene            complement(20442..21242)
                     /locus_tag="VF240_22065"
     CDS             complement(20442..21242)
                     /locus_tag="VF240_22065"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX7177962.1"
                     /translation="MLSPTSFLYEVRAGVAYVTLNRPERLNALTFEVYRELTDAFAAL
                     RSESEVRAVVITGAGRAFCSGGDVRDIIGGLQGRDVQGLLEFTRMTCELIRNIRGLRK
                     PVIASLNGTTAGAGACIALAADLRVASTEARIAFLFVRVGLSGADMGAAHLLPRYVGL
                     AKATELLYTGDFITAEEAHRIGLYNRVVAPEGLAEETRALAERLARGPAFGLAVTKEM
                     LDRELDVSLDTALELEAQAQAVCMLHPDYREAYEAFMEKREPVFKSDK"
     gene            complement(21335..21733)
                     /locus_tag="VF240_22070"
     CDS             complement(21335..21733)
                     /locus_tag="VF240_22070"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7177963.1"
                     /translation="MSFSVINPNSLGRPKGYANGLLTTPGARLLFVAGQIGWDENQRL
                     VSDDFVEQFDRALRNVLTVVGEAGGAPGGVARLTIYVTEMEQYRARTGEIGERWRALM
                     GRHYPAMALVEVKGLLEDGAKVEIEGIAVL"
     gene            complement(21824..>22612)
                     /locus_tag="VF240_22075"
     CDS             complement(21824..>22612)
                     /locus_tag="VF240_22075"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7177964.1"
                     /translation="KLADRTALVTGGGRGIGRAVALAFAREGARVMVVARTAAEVERV
                     AGEIRSERDGDDASAHHATCDVSDPESVRAAFAECAEAFGRPPDVLVNNAGIAESAPV
                     AKTSDEMWARHLAVNLSGAFYCTRAALPPMVERGWGRVINIASIAAKTGAPYIAAYSA
                     SKHGVLGLTRSAALEVARKGVTVNAVCPGYVETDMTTRAVANIAATTGRSAEDALETL
                     HRMSAQHRLVTPEEVAALCLLLASEDGRGITGQAINVDGGSVLY"
BASE COUNT         3771 a         7438 c         7689 g         3714 t
ORIGIN      
        1 tacgtccttg acctaccaga acacgacggg cgtcttcggc gcggcgcacc agtcggcaac
       61 ggccgtgcgc gcacaggtct tgcccgtcgt cgaagacttc cgcgtggccg ccgccaagac
      121 cgggatgctc ccgacgcgcg aggtcatccg cgaggtcgcg cgtctgttcc gcgagacgga
      181 tttgcccgca ccggtcgttg accccgtcgt gcgctccacc tccgggtatg acctgatcga
      241 cgacgaagcg ctcgccgcgc tcagagacga actcctgccg ctcgcgcgtc tgctgacgcc
      301 taacatcccc gaggccgagc gcctgtgcgg gatgcggatt gaaaatcgcg cagggatgct
      361 ccgcgccgca cagtcgctcc gaggcatggg cgcgcgcgcc gtgctcgtca agggcggcca
      421 cccgttcgtc gccgacggcg gcgacgccgc ccaagaggcc gcagaccttc tgctcgaaga
      481 ggacgagcgc gcgctcattc tcagcgcgcc gcgcgtcgcc tccacctcga cgcacggaac
      541 gggctgcacg ctggccgccg ccatcgccgc ctgcctcgcg cgcgggatgg agttaaagga
      601 ggcaaccgca acggccaagc gttacgtcac agaagccatc cgccgcgcgc cgggcctcgg
      661 gcgcggccac ggccccgtca accacgccgt cgtcccgcgc gtcggggcgt ggggcgcgct
      721 cgggagtgaa gagagatatg atgaagtctg aaaagttctt cgacctccga acggcgcttc
      781 gcgcggcgac gcgccacgcc ctcgcggcgt gcctgctgac ggcggcggcc gcgacgctcg
      841 cgcgcgcgca gggcgacttg acgggcggca tcggccagtt cgccgccgtc cagccgaaga
      901 aggagaagca ggcgaagcag gcgcggccga aggtcgtcaa gccggccgcg tccgcgtccg
      961 tcccccgcgt caatgccgcg cacgaacaac tcctgcgcgc cgccgccgcc ggcaacctgc
     1021 aacagacgca ggcggcgctc gccgccggcg gcaaggtcaa ctacaagggg aagacgggac
     1081 tgaccccgct gatgctcgcc gccgacgccg gctccaaaga ggtcgtcgcg cttttgcttg
     1141 cgcgcggcgc cgatctggcc gcgcgcagcg ccgagggctg cacgtccctc atctacgccg
     1201 tcgccggggc gcacaccgaa ctcacgcaga ctttgctctc gaagggcgcc gacgtgaacg
     1261 cgcgctgcgg cgacgggctg acggcgctga cgctcgcggc cgaggacggc gacgcaacgg
     1321 ccgttgccac cctcatcgcg cgcggcgcgg acgtgaacgc cgcctccgcc gacggtcgca
     1381 cgccactgat gttcgccgcc tccagcggcc acctcgaaat cgcgcaggcg ctcctcgcca
     1441 aaagcgccga cgtgaaggcg cgtcaacgcg acgggcgcac gccgctgcac ttcgccgccg
     1501 ccaacggcca cgggacggtc gccgcggcgc tcatcgcgcg tggcgccgag gtggacgcca
     1561 aacccgacga cggctggacg ccgctgctgt gggcctcgtg gtacggccac ctgtccatcg
     1621 tcaaagacct cgtcgagcgc ggggccaaca tctccgcgcg cgaccgcgac ggcgacacgc
     1681 cgctgatgaa ggcggccacg caggggcaca ccgccatcgc gcagctcctc atcgcgcgcg
     1741 gcgccgaggt ggacgcgcgc ggcaacgggc agggcacggc gctgatattc gccgcctcgc
     1801 gcggcgtccc ggagttgatc gcgctgctct tcgagaagaa ggccgacgtg aacgcgcgca
     1861 acgacgcggg gctgacgccg ctcatcctcg ccgcctcgca cggtcgcgtc tcggtcgttc
     1921 aggatttgct ccaacgcggc gccaacccca acgccgcgac cgccaagggc tacactgcgc
     1981 taatggccgc cgccaacaag ggcctcgcgc agaccgtcag ctcgcttatc gcgggcggcg
     2041 ccgacttaaa cctcaagtat gaagacggcc gcaccgcact gtccttcgcc acgcagaacg
     2101 gtcaggacaa agtgatccaa atcttgaagc aggccggggc aaagtagttg tagcagcacc
     2161 ggcgcacggc cgcgccagca cggcgcgcgc cgacggccgc gcgggcctta tctgatgatt
     2221 atgtcttcac aacccacagc cccgacggcg ttcggcccac gcggccgtcg gcgcgcgttg
     2281 tcgcttctcg ccctcgtcgt tttgacggcc gccgtctctt cctgtgccgg tctttcggag
     2341 cagggagctt cgacggggac gacgaatgcg ccgaatgggc aggccgtcgc caataaccag
     2401 ccggcacccg gcacactgag agcgaacgcc agcgcgacag tccccggcgc gcccgccacg
     2461 tcgccgccgc aggtcgcccc gacgccgggc cgcatggcct tgcccgacgc cgtcaagctc
     2521 gccgagataa acgccctcga cggagcgccc attcgcttct ccgagttcgg cggcaaggtc
     2581 gtcgtcctca acctgtgggc gacgtggtgc ggcccgtgcc ggctggagat tccgcatctc
     2641 gtggagttga acaaagaata cgccgggcgc gtcgagttcg tcggactgac gacggaagac
     2701 cgggcggcga gcgagccgct ggtgcgcgag ttcgtgcgcg agtacaagat ggactaccgc
     2761 gtgggcttct caggcccgac ggtcgccctc gcgctcatgc agaaccggac gagcatcccg
     2821 caggtcttca tcatccgcga cggttatatc cactaccgct tcatcggctt caacccgcag
     2881 atcagccccc cgcgcctgcg cgagggcatc gaggccacgc tgaaggccgg cgcttagagc
     2941 gactttcatc tgagggtaag gggtaaaggg gaaggggtaa gggagaagaa tcggtctttc
     3001 ccttacccct tcccctttac cccttaccct ttcatccctg cgtccggctc tactcgcccg
     3061 gcccctcttc tatcgtgacc ttctccatct tcacctcgtt gaggggacgg tcgccgcccg
     3121 cggtcggcgc ggtggcgatc ttgtccacca cgtcctgacc ggcgacgacg cggccgaact
     3181 tggtgtagct cggcgggagc gggtagtcgg agtgcatgat gaagaactgc gagccgttgg
     3241 tgttcgcgcc ggcgttggcc atcgccacgg tgccgcgctc gtaaggcccc cggtagagcg
     3301 gcgtggaggc attgatttcg tcgttgaatt tgccgcccca ggccgaggcg ccgccgcggc
     3361 ccgtgcccgt cgggtcgccg ccctgaatca tgaagccctt gatgacgcgg tgaaagatca
     3421 cgccgtcgaa agcgccgcgc tcggccagca gacggaagtt ctcggtcgtc ttcggcgcgt
     3481 cctcctccaa caactcgaag cggatcgtgc ccatgttcgt ctctattcgt gcaatgcggt
     3541 tcggcataag aatcctttca ttaagaggga gtcaggagtc aggagtcagg agtcagaata
     3601 aaagcatctt tactttcttc attctgactc ctgacttctc actcctgact ccccgtgttc
     3661 cgtaagaacg aatcgttcaa tcgcctcggc cacgccgccc tcgtcgttgg tggccgtgcg
     3721 gtggacgtgt ttgcgtgtga gcagcgactg ctcggcgttg cccatcagga cgccggtgcc
     3781 ggcgtattcg agcatctcca aatcgttcaa attgtcgccg acggccatca cgtcggcgcg
     3841 cgccagcccc tgctcggccg ccgcggcggc gacgcccgcg cccttcgagg ccagcgggtg
     3901 gagcacgtcg aggagcgcga agtccatctt ctgatagagc gtcagcagca gcttgacggc
     3961 gccgcccaac tcgccggcga gcgactcgga gagcgcactc atccggtcgc aggcgccgga
     4021 gaaagtgacg tggacggggt catggtcgag atactcctca agcgacggga cgaggcgcac
     4081 ggcctcgtca gcttcggggc cgtggatgcg ccgcgcccag gcgacgtact tggcgagcgc
     4141 gacgttacct gcgctcaggt ggtcgtagac gagcacgcct aagccgtggt ggtcgtcgct
     4201 gacgagcgca tcctcgccgc gtgcgcgccc cgcgcgggcg acggcgagcg cggcctcctt
     4261 tggcaggagc aacgccgcca cggtttccag agtgcgcgcg tgcttggtga gcgcgccgtt
     4321 atgcgagatg acgggcacgt cgagtccgag ttcgagcgcg agggggcggg cgtcgcggaa
     4381 gcgccgcccg gtgacgaggg cgatgcggac gccgcgctcg cgtgcgcgct cgatggcgcg
     4441 gcggttgcgc tccgggaggt ttccgcgcga gtcgaggagc gtcccgtcga ggtcgagcgc
     4501 gatcaggcgt atggacatta aaaagctttc agcaattagc tctcagcttt catctataac
     4561 catcagccgc gccgaacgcg tgagcaaaac cgggccagcc gccgcccaca aaaaagctca
     4621 ctgctgatag ctgactgctg aaagctttta ataaaatcgg taaactgaat ctaacatccc
     4681 tccgagagag gtgcaacata agtgctccaa ctggcgacgc gcgcgcgtcc gtggggtgcg
     4741 tgcggcgggg gcgtgaatcc tcgcgtcggc cgagcgcgcg aaggcttgag gggcggcggc
     4801 ggcttgcgcc ggccgaaagg ccgggggata cgtggaaatc ctggtctttt gtccgaatcg
     4861 gctctgttga aagggttcac cgggaatgaa tgtcacacac cttgaatgcg ccgcgtgcgg
     4921 cctgcggcac gaggcacggc ggttgcacaa cctctgccgg gagtgcgccc ggccgctgct
     4981 cgtgcgctac gatttgcgaa gcgcgtcgga gactctgacg cgcgaaagtt tgaaggatag
     5041 aagggctgac ttatggcgtt accgcgaggt gctgcccgtc gagcacgaag aaaatatcgt
     5101 ctcgctcgga gaaggctgga cgccgctgtt gcgcgccgac cgtttgggac gctcgctcgg
     5161 tctgcgtcat ctcttcatca aggacgagtc gcagaacccc acgcagagct tcaaggcgcg
     5221 cggcatgtcg gcggccgtct cgatggcgaa ggagctgggc gtgactaaga cggccgtgcc
     5281 ctcggccggc aacgccgccg gggcgctcgc cgcctacgcc gcccgcgccg ggatggaagc
     5341 gcacatcttc atgccacgcg acacgccgcg cgccaacgtc gtcgagtgcg agcagaccgg
     5401 cgcgagtgtg acgctgatgg acgggttgat cacggactgc ggcgcggagg tcggccgtcg
     5461 caaggacgcc gaagggtggt tcgacgtttc cactttgaag gagccgtacc gcgtcgaggg
     5521 caagaagact ctcggctacg agctggctga acaactcgac tgggagttgc ccgacctgat
     5581 catctacccg acgggcggcg gcacgggcct catcgggatg tggaaggcgt tcgacgagat
     5641 ggagcagatg ggctggatcg gcgggaagcg tccgcgcatg gtgtcggtgc aggcggccgg
     5701 gtgcgcgccc atcgtgcgcg cgttcgaggc gggcgtgcgg cacgccgaag agtttcagga
     5761 cgcagcgacc gtggcgtccg gtttgagggt gccgcgcgcc atcggcgact ttctgattct
     5821 cgacgcgctg cgggcatcca acgggcgggc catcgccgtc tcagacgagg aactgctcgc
     5881 ggccgtcgga gagatcggcg cggccgaagg gctgttcgtt gcgccggagg gcgcggcctg
     5941 tctgccggcg ttaaggcgtc tgatggcgga cggcgcggtg ggcgaggatg agcgcgtggt
     6001 gcttttcaac acgggcgcgg gcgttaagta tctggagagt ttcggagggt aggagattgc
     6061 gaactgtagg ggcacggctt gtccgcggcc cgctcggccg aaagaattaa catggatgga
     6121 caggataggc aggatgaaga aggagctgat ttcagatttg agatttaaaa tttcaaatct
     6181 cttatcttgg tcatcccgcc catccatgtt aattgcttcc atcgttgagc gggccgcgga
     6241 cacgccctac ccctacaggg aacactcgcg gcggtggatg aagttatcgg aaggggatta
     6301 agtttatgat gtggcgaaga cttgggccgg tgttgctgtc ggccgtgttc ctgtcggtct
     6361 gcgcggcggc gcagacgggc agatcggcga tgaagggcag cggcgcttcg cagccgccga
     6421 agccggccgc ggcgggcgag gcggccgatt ccacgcggct cgacgcgttg cgcgccgagg
     6481 gcttcgaggc gctttacaat ctcgattatg agcgggcgcg cacgaagttt aaagaagtcg
     6541 cgcgactgtt tcccgaccac ccggccgggc cgcagttcct cgccgccacc gtctggctga
     6601 agaccctcaa cgagtcgcgc cgcctgcaag cctcgctcta caacaccgag ggcttctacg
     6661 cgaaggctaa agaggaggcc gacccgaagg tgctcgccga gtttcgtgag ctgacggcgc
     6721 aggccaagac gctcgcgcag gcccgcctca agcgcgaccc gaaggacgcc gaggcgcttt
     6781 acttctacgg cgccaccgag gggctgaagg ccgccttcgc cgcggccgtc gagcgcagct
     6841 tctactcggc ctggcgcgat gggcaggagg ccgccgaccg tcaccgcgaa ctcctcaagc
     6901 tcgaccccaa ctaccacgac gccggcatca ccgtcggcat gtacgactac atcgcggcca
     6961 cgctcccgct cccttttcgt atcggcggct cgctcgtcgg cctgcgcggc tcgaagaagc
     7021 gcggcctcca gacgctcgaa cgcgtcgccc gcgagggacg ctgggcgcag gacgacgcga
     7081 aggttctgct catcgccctc tacaagcgcg agggccgctt cggcgaagca ctcaccttcg
     7141 cgcgcgaact ctccgcgaag tatcaacgca actacatctt caagatggag acggccgacg
     7201 cgctcgtctc tctggccgcc gcggcgcgcg cgtccgaccc ggcggcggcg ggcaagtcag
     7261 agacggaagc ccttgccatc ttcgaggcgc tccttcagca gaagccgtcg aagggtgagc
     7321 cggcgcggcc gctcgacctg atccacttct cctacgggca ggcgctcgcc gtcgccggcc
     7381 aacacgagcg ggcggcgaag gaatacctgt tgggcgccgc ggcgccgggc gccgagcggg
     7441 gtctcgccac gatgtcgcgg ctgcgggccg cgcagtcgct cgatcaggcg ggccgacgcg
     7501 aagaggcgca ggcgcagtac agggaagtgc ttacgcgtcc gaacgtctac gactcgcacg
     7561 aggaggccca gcgcgggctg aaggagccgt tcaagccgcg cgcgggcggg gcggaagact
     7621 cgggcgggcg cgccggacag aagacggccg cggggtctga gtagcgaaaa ttcaaggggc
     7681 gagccggggg tttcaattga tgagcgagtg ttgttgtagt ctgccacacg ggttcgcggc
     7741 gatgtctgcg ctacagcgcg cgtcggccga gatggtcggc gcgcgtgcgc gtccgcgtcc
     7801 ggtctccgtc acgtcggcgc gtccgcggcg gttgctgttg tgtctcgacg gcgtgcccca
     7861 cgaggtcatc acggcggcga aggggcgcgg gatgttcgac agctttggcg agccttcgcg
     7921 gctgctctcg ccgtttccca cgatgacgaa cgtcgcgctg gcgctgatgc ttcgggccac
     7981 gccgccggcc ggctacgaaa gtctctactt cgaccacggc gagcgccgcc tctcgggcgg
     8041 ggtgcgtaag tacatcggcc gacgcacgcc cgacaagatg ccctcgtcct acatggacga
     8101 actcgactat caggagccgc tcgccttcga gttcctcatc tacgtcgcca ctgaaagggt
     8161 gtggcgcgcc gacctccgac gcttccgcga acgcttccgg gccacgtcgc ccgcgcgcga
     8221 cttctttggc ttcctcaaag cgaccgacgg attgctgcac aagtgcggcc cccgaagtct
     8281 ctcggtcgcg ctccgctcgc tcgaccaaat cctgcgcgac attcaagagg cgtgcggctc
     8341 ggagaccgag atcgtgctct tctccgacca cggcatgaac cttgaggaga accggcgcgt
     8401 ccacctgcaa gagggactgc gccggagcgg cttcgaggtg acggccgacc tgcgcggagg
     8461 gcgcggccgc gcgcgcgtct ccacgcccgc gttcgggctg tgcagctacg ccgcgctcta
     8521 ctgcggcgac gagaccgacc cggcggaagt cgccgccgcc acgggaacgc tcgaaggcgt
     8581 tgacttcagc gtgaggcgcg acggggaagg gacaggcgtg ctcgtcgagg gcgcgcgcgg
     8641 ccgagcgcat atccgacgcc gcgaacgggc cgcgggcgtc gcgtcttaca gctacgagtg
     8701 cgccgatggc gacccgctcg gcctatcgcc catcgtgagc gcgctcgcgc aagaagggtt
     8761 gctcgacgcg caagggttcg cccccgacgc gacgtggcta gagcgcacgg ccgaacacat
     8821 ctacccggac gcgcccgcca acctccacgg ctcgctctac gcgccgcgcg tccggcacac
     8881 cgccgacctg ctcctcagcc tccacgacgg ctacttcttc ggcgacaccg ccttcagcca
     8941 catgatccgc ctgcgcgcca cgcacggcaa cgcgctccgc tccagctcga acgccttcct
     9001 gatgagcacg caccgcacct tcgccccaca cgtccgcgcc gccgacgtgc gtccgctgct
     9061 gcgtgactga agcgatggcc cacgcgccgc gcgcctcacc tgctctcgtc gcgcttcggc
     9121 ggcgtctgcc agagcacgtt gatgatcttc cagcggccct catacttggc cagatgcatg
     9181 tagtcgatgc cccagatggc cgtgagcttg atgctggcgg tctggtcgga gatgtcgtaa
     9241 atgacgattt ccttcggcgc gtccttcttg acgtggccgt ccttgttgta agacttcgcg
     9301 agatcgacga gctgctggaa ggtcatcgtg tgcgggctgt aactggtttc gcccttcttg
     9361 acgtagaagc cgcgcttggc gagttccggg tggatgctcc gctcgatgcg cgcgggggcg
     9421 atctcgtaga cgccctcgac gtagtcgagc acggcttgtc tgacggcctc ccgctcggcg
     9481 tccgactgcg ccgcggcggc cactgacatt gacaggacga gtgcgacgct taaaacgatc
     9541 cttctcatca tcgactaaac cctccgattt ttaagattga ttgtggggaa cccaattgga
     9601 gaaacgcgcg gcggccccgt ggtgttccgc acaaccggcg cgctctcgct cgcaccacgc
     9661 cgcgtattct accccaacgg agccggacgc ggcttgaccg ccgccgcgcg cggcacgtac
     9721 aaatgccgct atgacgacag acgacacggc ggacgctgag cacgcgccgg gcggcgcggg
     9781 cgcggccgtc gagaccttcg agttgacgcg acgcttcggc gagttcgcgg ccgttgaccg
     9841 tctcgacctg aacgtggcgc gcggtcagtt cttcggcttc ctcggcccca acggcgcggg
     9901 gaagtctacc accatcaaga tgctcacggg gctgctcgcg cccacctcgg gccgcatccg
     9961 cgtgctcggc ctcgacctcg aagaccaccc gctcgatgtg aagcgacgca tcggcgtcgt
    10021 acccgaagac ctcaatttgt tcgagcgcct gacgggcgcc gagatgctca ccttcacggg
    10081 ccggatgtac ggcttggcgc gcgccgacat ccttgagcgc gcgcgcgagc tgctggagct
    10141 gatggagctt gactcggagc cgcgcaagct cgtcatcgaa tactcgcacg ggatgaagaa
    10201 gaagctctcg ctcgcctgcg cgctcatcca ccgccccgag atactcttcc tcgacgagcc
    10261 gttcgagggc gtggacgcca tcgcctcgcg cacgctcaag gacttgctgg cgcgcctcac
    10321 ctcgcgcggc ctcaccgtct tcctcacctc acacgttctg gagatcgtcg aacgcctctg
    10381 caccgacatc gccatcatca gcggcggacg gctcgtcgcc tcggggacgc tcgacgagct
    10441 gcgccgcggc atcagcgtcg aaggcgacgg ccacgccgcc tcgatgaccc tcgaagacta
    10501 cttcatccgc gtcgtcggcg gcgcccgcac ctcgggcgac gaagtgctgc aatggctggg
    10561 atgaagaaga gtgagcagta agcagtgagc agtcaacgag ccgccggagg cggcggaaga
    10621 ttttagccca cggcgcgagc cgtggggaca cgtcggataa aacttagtaa gccccggagg
    10681 ggcgaaagaa tctcgacggg cgtgtgacgc ggcctaagat tctttcgccc ctccggggct
    10741 tcacattttt tgttgcgccg ccaccacggg cccagcgccc gtggctacac tctgccgccg
    10801 ctccgcggct cgttgactgc tcactgctca ctgctcactg ctcactgcct gtagaccgga
    10861 tatttcggcc ggtgcggggg cgtgggcggg cctttcttct tgagttgttc gagggcgatt
    10921 gctacggcgc gttcgagttg cgggtcgcgg ccgcggcgcc agtcggcagg catgttttcg
    10981 agtatcacgt cgggcgtcgt gccgcggttt tcgacgagcc aggtgccggc ggcgtggtcg
    11041 taccagcccc agtcgggcac gcgcacctga ccgccgtcga gcaggttgtg atccgtggcc
    11101 ccgacgcccg cgcctgaggt gcgcgtgccg acgaccgtcg ccacgcccgt gcgcttcgct
    11161 atccacggca gggtgtctcc gcccgagccg gcgctctcgt tggtgagcac gaccttcggg
    11221 ccgtcgatga tgccggtggg gatgcgtatg tcttcgccgg cgcgcagggc ggcgcggtag
    11281 agcggcgcgc ggcgcagcgt gtcgatgacg tagtcggcgg cgcgcccgcc ttggttgaag
    11341 cgcgcgtcga ggatgagacc ctgccggtcg agctgcgcgt agaagtcgcg gttgaaggcg
    11401 ttgtagccgt cgtcggaggt gttggtcatg tagacgtagg cgagacgccc gcccgacagc
    11461 tcctcggcgc gccggcggtt ctcgcgcacc cactcgatct gccggagcgc atactcgctc
    11521 gccgccggca ccacacgcac ttcgcgcgcg cccacgccgt cggcccgcgc cccgacgcta
    11581 atcttcaaag gcttcgacgc acggttcaca aagtggcgat acaaattgtc gcccgccctg
    11641 acctccacgc tctcgacggc gagaagatac tcgcccgcct tcacacccgc gcccaggccg
    11701 tgctgcgcga gcgggccggt gacgccgagc acggggtcgc cgcgcaggac gcgacggatg
    11761 cggtagcggc cggagtcggt ctcgtagtcg gccccgagca gtcccgtggt ctcggcctca
    11821 agcccctgcg gcgcggggct atcgccgccc cagatgccca tgtggctcgt cgagaggtgc
    11881 gagaatgtct ccttgaagac ttcgttcagg ccgtcgcgcg tgacgacgtt cggaaggtac
    11941 gcggcgtagt gacttttgag ttcgcctgcg tttatcccgt gcaggttggg gtcgtagaag
    12001 tagacgcgca ttcgccgcca cgcctcttcg tagagcagac gccactcggc gcgcgggtca
    12061 atcgtcagct ccatagactt cggcgtgaag cggccatcgt ccggcttcgg cggcgcggcg
    12121 tcggccggcg ccatcgccca gcggccctga cgacgcagca gcaggtgctt gccgtcgggc
    12181 gtgacgcggt agttgcccgc gccctcgacg aacttctccg tgccctgtgt gcccgcgcgg
    12241 aacttgtgga gcactactcc ctcgacgacg aacagcgtgc cgtgcgtccc ggccatcaga
    12301 cggccggggt tgccgacgcc gatgcttagg ggcaacacgc gctcggcgat gccttcgggg
    12361 tcgatgtgga tggtgcggac gcgcgccacg tcatcgtcgc cggcgcgcgg cgcgagcggg
    12421 gagggcgact cgcggttgag gacgacggcg tagagcgagc gtgtgacctg cgagcggaag
    12481 gggaagccgc tcatgctctg cgactcgacg aggccggtct gattgctgcc gatgaagtag
    12541 aggtattcgc cgccgcggtc gaacgtgggc tgctgcgcgt cgatgcgctg gtcgctgacg
    12601 gcgtaagtct ttcccgtctc cagcgagaag agcgtgacgg tgcggacgcg gttcacgcca
    12661 tacttcgcgt aggcgagcca gcggccgtcg ggcgaccacg ccgggtgcat gaaggtgccg
    12721 ccgtcggtgt gcgtcgaggc atcgacgcgg cgcacggcgc ccgtcgcgag gtcgaggtag
    12781 cgcaacgtga gcaaagcgtc cgtgaaggcg agcttcgacg agtcgggcga ccacagaagc
    12841 tcggcgtaga tggacgacgg cctatcgatg gtgaagcgac gcaccgcgcc gacgacggca
    12901 gaatcaacaa cgcgcgccgc gccgccactt gtcgcgccgc ggcccgccgc ggagttgcca
    12961 gatgacgggg cgagggtgcg gacgtgcaac tccaactcgc ccgactcgtc ggaaaagtaa
    13021 gcgagccagc ggccgtcggg cgaccagacg gcgccgcgct cggcggcggc gggggtgcgc
    13081 gtgagatttg tgacctcgcc gtcggagacg cgcgccgtga gaatctcgcc gcgcgcgccg
    13141 agcaggagtt gcttgccgtc gggcgagggg aggatgttat cggccgactg gccggcgtca
    13201 atcgtgcgcg gcttcgtctg tgggaagtcg cccgcgacgc gcacgtccac gcggcgcgtc
    13261 tggttggtct gcgggtcgaa gaggtgaagc gcgccgtcgt ggacgagcag gatggagtcg
    13321 ccgccgtggg aggcccagcg cacgtcgtat ttttcaaagc gcgtcagttg gctgacggcc
    13381 ttcgtccgcg tgtcgtagac gaagaggttg taggggccgg cgcggtcaga caggaagtag
    13441 acgcgctcgc cgacccagat ggggttgagg tcgttggcct cgccgcgggg tatctcttcg
    13501 acgacgctgt cggagagacg cgcgagccag attttcgaca tggcgccgcc gcggtagttg
    13561 cggaactgaa aaacctggag cgggtcgagc agcggcgtgt aggcgaggcg cgtctggtcg
    13621 ggcgagaagg agccggccca acctgacggc agcggcagcg gctcctccgg tccaccgtcg
    13681 gcggggacgg tgtagaggcg cgcgctgata gagctcatgc ggtcgcgcag cgtgtagaag
    13741 agaacgctct taccgtcggg cgtccacccg gccggggcgt caagctcggg gtggtaggtg
    13801 agacgcacgg cctcgccgcc ggcggccggc atgacgtaaa cgtcccaggc gagcggcccc
    13861 gacacggggt tgtggcgcgc gaaggcgatg cgcgagctgt cgggcgagta gaaggggagt
    13921 gagtccttgc cggggtagtc cgtgagccgg cgcgcgtcgc cgccctcgcg cccgaccgtc
    13981 cagatgtcat cggcgtactc gaaggcgacg tgcgtgcggc tcaggctcgc gccgcgcagg
    14041 ggcagcggcg acgtgacggc cgtctgcgcg cgcacgacgg cggcgccggc gatgatgacg
    14101 agtgcggcgg ctagcgcgaa gagtctcagt gttggggtgg gcaaggttgt cctccttccg
    14161 ccgatgcgac cgctgtctgt aaagggagag ggcggggcat tggccgaagc cggtctgaaa
    14221 ttagaacccc gcaggaggtc gcttcgtggc gaatgatgac aaaaaaacag cgcggctgac
    14281 aaggctcgcc gcccgcggcg tcgtgcctcg cgcgacgcaa aaactgttac actgcgcgcg
    14341 gcctaatgtc agtgttcagt tgatgggatg gaaagttgga ctcaccgcgg aggcgcagag
    14401 gacgcagagt tcacgcagag gcttgaatct ctctgcgccc cctccgcgcc ctctgcgact
    14461 ctgcggtgaa cctacttccg ctctcactca agtgaaagcg gctctaaact tatgtaccac
    14521 ccaccggaag ccatccggca cgaaatcccc gagacgcgct ggccgacacg cgaggaggcc
    14581 gcgggcgcgc tcctctttca acctgtcggc gtcggccccg tccgactcga aagccggacg
    14641 tgggtgccgg cgatggtgcc gtggcgcgcg accgacgacg gtttcgtcac gcgcgaaaat
    14701 atcgactggt acgcgcgctt cgccgagggg cggcccggcg caatcgtcgt cgaggcgacg
    14761 ggcgtgcgcg acatcccctc ggggccgctg ctgcgtatcg gacacgaccg cttccttccc
    14821 ggcctgcgcg aactggtcga gaccgtcggc cgcgcaagcg gcggccacac gcgtctgttc
    14881 atccagatca tcgacttcct ctccgtcaaa cgacggcccg agcgcgccaa gttcttcgaa
    14941 cgcttcctcg tcgtcgaaga tcgacaccga cgcggcctcg ccgccgcgac cgcggacgcc
    15001 ggctggctcg aacgcgacga gccggagttg cgcgcgttcc tcgcggcgtc ggccgacgac
    15061 ttagtcaggc gagtgctcga cgagcgcgag tgggaggcgc tggccttcgg ctaccgcgag
    15121 agcgttacgg acatgcatct cccgcacgtc cgagagttgc cacaagtgct gcccggcatc
    15181 ttcgccgccg ccgcgcggcg cgcgcgcgcg gccggcttcg acggcgtcga gctgcactac
    15241 gcgcatgcct acacgatggc ctccttcctc agtcgccgca acgaccgaca ggacggctac
    15301 ggtgcgacac ccgagggacg cgtccgtctc ccgctggaag tctaccgggc ggtgcgcgcc
    15361 gaggtgggcg cagactactg cgtcggcgtt cgcttcctcg gcgacgaagt gatcgcgggc
    15421 ggcagccgca tcgacgacgc cgtctactac gccgcggagt tcgcccgcgc cggcttcgat
    15481 tttttgtcgg tctccaaggg cgggaagttc gaggatgcca agcagccgaa ggtcggccat
    15541 gccgtctacc cctacacggg cgagagcggc tacgagtgta tgccgaccgt catctcggac
    15601 gcgcgcggcc ccttcggcag aaatgtgccg ctcgtgcgcg ccgtcaagcg cgcggtgagc
    15661 gagacgggac gcgcgacgcc ggtcgtcgcc gccggcggca tctccacctt cgagcaggcc
    15721 gaagcgatcc tgcggcgcgg cgacgccgac atcatcggca cggcccgcca gagcctcgcc
    15781 gaccccgact ggtttctcaa aacaaaactc gggcggggcg acgaggtcag gcgctgcacc
    15841 tacaccaact actgcgaggc gctcgaccag atgcaccggc aggtgacgtg caagctctgg
    15901 gatcgcgtgg ggctggacga ggcgggggtg agtttgagta tggacggcag acgcaggctc
    15961 ttgcctccgc gttgggatgg cgacaacgcg gaggcaagtt gagagcgagt tggaaagtca
    16021 tgcccgcggc ggcgcagggt cagcggagcc ggccgtgaat acgcagttgg acgaggtctt
    16081 cgatgttcaa gtctgcgttg ctcgccttcg cgcgccggat gaagtcgcgg tcgatgttga
    16141 aaatcttcag cttgacggcg tcttcaatcg acacctgtga gaagccttcg gccttcagct
    16201 cttcgaggaa gtcgggcgtg actttgaaaa tccgcagctt gacgagttct tcgagcgact
    16261 gcccggcaaa ccccatctcc tgaacctgcc gcgcgaactc ggcgtcaacc ttgaagatgc
    16321 gcgccttgac gagttcttcc agtcccagat tctcgaagcc gagcgacttc atctcggcga
    16381 cgtactgcgg agtcaccttg aagatcaggg ccttgatggc gtcgtcaaac gacagccggt
    16441 cgaagcccac cgacttcaaa tcatcaacga agctcacggt caggttgagc agcgtcgagg
    16501 tgagcatctc gcggtcggtc aaatcgccgt agccgcgact gctcatcgcg ctcctgaaac
    16561 tctggctcgg attgagcagc cacgtccccg tgccccggcc gccccggaag ctgcctttac
    16621 actcgacggt tcccgcctca cgcaccagac gaaaactgac cggcgtgtcc gtgcgcgccg
    16681 atatctgttc acgcgtcaga ccctgaaagt cggcgagcga gtagtcggag ccggaaaagt
    16741 ttccgctgcc gcgctgggcg tcggtgcgga tatcaaactg aagtcgctcg cccgccttgt
    16801 cgtctcttaa ttccgccgtc cacgtgccgg agaacgtcga ttgtgacgct cccgcggcgg
    16861 cgtgcgggcc gcccgcggcg gctgtgtcgg cgcaggccgc gccgaaagtg aagagggtga
    16921 acaggctgag cgcgatggcc acgcgggccg ccggcccagt ctgcgatgcg cccgccggta
    16981 tcctgcgcgc gcctgtcgaa gaagcttgct tcataaaaca cctctagccg ggaaatttaa
    17041 caacgagact tctgtcctct ctatcaataa acaccgcggc gcggcgagtc ctgacaaaaa
    17101 atctcgggtg gaaaaagaga atggatggag gccggaggat taattcgcgg ggctgacggc
    17161 gcgctcgtaa gcctcgacga acgcggggtc gcgccgccgc aggctctcga aatcgacctt
    17221 gccgctgcgc gtcatcaggc tgtaggcgaa ctgaagcggc ggcaggtgca tgtagtcggc
    17281 ggcgcgctcg aaccagaggc ggctgtcgtg agccgcgcgc tgatactcct cgatgacggg
    17341 cttgcgcgtg cgctcgaact cggccagcgc ctcccggacg ccgcgccccg ccgcgaagcg
    17401 ctcgaagagc gcgattgcgt cttcgagcgc gagcttggtg cccgagccga tggagaagtg
    17461 tgcggtgtgg agcgcgtcgc cgagcaggac gacgttctga tggaaccaac gctcgttgcg
    17521 gacgagcagg aagttgaccc agcgcgagtt gttcgagagc agttcgtggc cgccgaggtc
    17581 gctgtcgaaa acttcgccga ggtaggagcg cgccgcctcg tccgacatct cggcgaaccc
    17641 cgcgcgcgcc cacgtcttct cgtcacactc gacgatgaag gtgctcgtcg cgcggtcgaa
    17701 cttgtaggag tgcgcggcga agagtcccgc ccccgactca cggaaggtga gcgtcagccc
    17761 gtgaaaaagc tgtcgcgtgc cgtaccagac gtacttgttg ccgcctaacg cgagcgccgg
    17821 ctggaactct tccgcgaacc gctcgcgcac gcggctgttg acgccgtccg cgccgacgac
    17881 cagatcggag ctgcccgcca gttcgtccac atcggcgact tcttcccggt agcgcaggct
    17941 gacgcccagc ccgtcgcaac gctcctgaag gatgttcagc agccggaggc gcgcgatgcc
    18001 ggagaagtga ttgccgcgga tcgtgacctt gtcgtcgccc ttgaggacat cgacgttgtc
    18061 ccacgtctcg aagctctcgg tgatcgagcg gtacgtctct tcgtcggccg cgcgcaggtt
    18121 cgagagcgtc ttccccgaga agaccacgcc ccaaccgaac gtgctgtcgg gcgcgttcct
    18181 ctcgaagacc gtaatctcgt gcgagggcca gcgcttcttc atcaggagcg cgaagtacaa
    18241 ccccgcgggg ccgccgccga tgacgttgat tctcatcctg tattctcagc tcgcctcgct
    18301 cagcactctc tccagcaagt cattaagatt cagcttcgcg gcccatatcc gcaaatactc
    18361 aacgtccaaa ctctcgccct gagttccgat gacgcccaga acatccctcc attgcatgtc
    18421 agacccttcg ccgccggccc gataccactt gagctttgac agcacggtgt cctcggccgt
    18481 tgcgacgtaa attttctgat tgacgtcggg ggcgattgtc tctagttgcc gccgcgccag
    18541 ttgttgcacg ctgaagtcgt ccgccggggg gatgaaaacg tcaatcttga aaactgagtc
    18601 gaagtgaata gcgttgaatg agccgtgtcg cgcaacggcg cggcgcacgg ccaagtcgtc
    18661 aacataaaaa tcttcttgca gcgcagcgac gagcggtttg acatgttcgg ccttgatgtc
    18721 ggccatgatg tcgatgtcac cggtggcccg gtaaaggccg cgcatcgagc tggcgaaaga
    18781 cccgactaca acgtagctga tgcccagctc gtcgagcacg ccggccacct gtgacaaaac
    18841 tatgaaaggg gtttccatga gtcatcggcc atgcgctaga tggctcacat ttccggggtc
    18901 gaagccgtag gcgcggatga cttcgtcgcg cgggagtagc tcggcaatca gtctgcgacg
    18961 cagttcttcc tcgtcggcaa gagggaagcg gcgacgcaaa tcggccagca tcagcaggcg
    19021 ggtcgcctga atgagttcac acgtcagggc gagccgcttc cagacgggcg tgcgacgcat
    19081 cagcttaaac atcatctgct gcgcttcggg cgtcgtatct ggcgagagag gtttcattgt
    19141 ctaatcggtt tgcaagctca cagcggcaac acacggaaag cagccggtca atctttcaag
    19201 agttggttgg cgatgacgag cttttgaatc tcggacgtgc cctcgtagat tctgagcgcg
    19261 cgcacgtcgc ggtagaggcg ttcgacgacg tggccgcggg tgagaccctg cgcgccgtga
    19321 atctggacgg cctcgtcgat gatgcgttgc gcggcctcgg tggcgaagag cttggcctga
    19381 ctggcggcgc gggcgagcgc ggagtcggtg gccgtgccct cgtcgcgcag atgcgcggcg
    19441 cgatagacga ggaggcgcgc ggcgtcgagg tcggtggcca tctcggcgag cttcgcctga
    19501 acgagctgga aggaggcgag gggctggccg aactgccgac ggctcttggc gtggcgcagc
    19561 gactcgtcga gcgcccggcg ggccatgccg caggcggcgg cgccgacgct cgcccggaac
    19621 gtgttcatcg tctgcatggc gagccggtgg ccgtcgccct ccgcgcccac gcgatcctcc
    19681 tcggggacgc gcagggcctc gaactccacg acgccgatgg ggtgcgccgc gatgaggcgc
    19741 gtgcgctcaa cgacgcggaa gccggcgaga cgcgcgctga cgacgaaggc ggagatttcg
    19801 gggcggccgt cggcgcgcgt gcccgtgcgc gcgaagacgg tatagaagtc agcgaggccg
    19861 gcgttcgaga taaagtgctt gcggccgtcg atgcggtagc cgtcgccgtc gcgctgtgcc
    19921 gtcgtttgaa tcgccgacac gtcggagccg gcctccggct cggtgagggc gaaggcggcg
    19981 attgacttgc cggtgctggc gcgcgagagc cagaagtcgc ggatgtgttc ggcgccggca
    20041 aggctcaagg cataggtgcc gaggccctgc atgacgaagg cgaggtcggc gagcggcgac
    20101 gagtaggcga gcgcctcgcg aatcaggcag agcgagcgca cgtccacggc cggcgtgttg
    20161 tcgagctgcg ggacggagta actgagaagc cccgtctgcc caagcaaccc cagaagctcg
    20221 cggaagtgcg cgtcgccgtc gcgctcctcc tcgtcgccgg ccgcgcgccg ctcgatgtcg
    20281 gtggcgacaa actgcgccac gctcgccgcc agccgccggt gctcgtcggt gaaaaagggc
    20341 gtctcttcgt aaaacaggtc aggcatagtg aaaaccgtga taagtgatga gtgatgagtg
    20401 ataagaaaga gcagacagct tttcttatca ctcatcactc atcacttatc actcttgaaa
    20461 accggttcgc gcttctccat gaaagcctcg taggcttcgc ggtagtcggg gtggagcatg
    20521 cagacggcct gcgcctgcgc ttcgagttcg agggcggtgt cgagcgacac gtccagctcg
    20581 cggtcgagca tctctttggt gacggcgagg ccgaaggcgg ggccacgggc gaggcgttcg
    20641 gcgagggcgc gcgtctcctc ggcaagcccc tcgggcgcga cgacgcggtt gtagaggccg
    20701 atgcgatgag cctcctcggc cgtgatgaag tcgccggtgt agagcagttc ggtcgccttc
    20761 gcgagtccga cgtagcgcgg cagcaggtgc gccgcgccca tgtcggcgcc ggagaggccg
    20821 acgcggacga agaggaaggc gatgcgcgcc tccgtgctcg ccacgcgcag atcggcggcg
    20881 agcgcgatgc aagcgcccgc tccggccgtc gtcccgttca gggaagcgat gacgggctta
    20941 cgcaagccgc ggatgttgcg tatcaactcg cacgtcatcc gcgtgaactc caacagcccc
    21001 tgcacgtcgc gaccctgtag accgccgatg atgtcgcgca cgtcgccgcc cgagcagaac
    21061 gcgcgccccg cgcctgtgat gaccaccgcg cgcacctccg actcggagcg cagggcggcg
    21121 aaggcgtccg tcagctcgcg atagacctcg aacgtcagcg cgttcagccg ctccggccga
    21181 ttcagcgtga cgtaagcgac ccccgcgcgt acctcataga gaaaacttgt cggtgaaagc
    21241 ataaaagagt gataagtgat aagtgatgag tgatgagaaa gagataagag gcgaccgacg
    21301 gtctttctta tcactcatca cttatcactt atcactacag tacagctatt ccctcgatct
    21361 cgaccttcgc gccgtcttcg agcaggccct tgacctcgac gagggccatc gcggggtagt
    21421 ggcggcccat gagcgcgcgc cagcgttcgc cgatctcgcc ggtgcgcgcg cgatactgct
    21481 ccatctccgt gacgtagatg gtgagacgcg ccacgccgcc cggcgcgccg cccgcctcgc
    21541 cgacgaccgt taacacattt cgcagggcgc ggtcgaactg ctcgacgaag tcgtcgctga
    21601 ccagccgttg attttcgtcc caaccgatct ggccggcgac gaacagcagc cgcgcgcccg
    21661 gcgtcgtgag cagcccgttg gcgtagccct tcggccgacc gagcgaattg gggttgatga
    21721 ctgagaaaga cataaagatt caagtgagca gtgagcagtg agcagtgagc agtctaaaga
    21781 gccggattgt ttttactgct cactgcttac tgctcactgc tcactaataa agtacgctcc
    21841 cgccgtccac gttgatggcc tgtcccgtga tgccgcggcc gtcttcggag gcgaggagca
    21901 ggcagagggc ggcgacctcc tcgggcgtta ccagccggtg ctgggcgctc atgcggtgca
    21961 gggtttcgag cgcgtcttcg gcgctgcggc ccgtcgtggc cgcgatgttg gcgacggcgc
    22021 gcgtcgtcat gtccgtctcg acgtagccgg ggcacacggc gttgaccgtc acacctttgc
    22081 gcgccacttc gagcgcggcc gagcgcgtga ggccgagcac gccgtgcttc gaggccgagt
    22141 aggcggcgat gtagggcgcg ccggtcttgg cggcgatgga ggcgatgttg atgacgcggc
    22201 cccagccgcg ctcgaccatc ggcggcagcg cagcgcgcgt gcagtagaag gcgccggaga
    22261 ggttgacggc gaggtgccgc gcccacatct cgtcgctcgt cttggcgacg ggggcgctct
    22321 cggcgatgcc ggcgttgttg acaagcacat cgggcgggcg gccgaaagcc tccgcacatt
    22381 cggcgaaggc ggcgcgcacg ctctcggggt cggacacgtc gcacgtcgcg tggtgcgccg
    22441 aagcgtcgtc gccatcacgc tccgacctga tctctccggc cacgcgctcg acctcggccg
    22501 ccgtgcgcgc aacgaccatc acgcgcgccc cctcgcgcgc gaacgccaac gccacggcgc
    22561 gcccgatgcc gcgcccgccg ccggttacca acgccgtcct gtcagcgagt tt
//
LOCUS       DASVES010000517         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1194251, whole genome shotgun sequence.
ACCESSION   DASVES010000517 DASVES010000000
VERSION     DASVES010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194251"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VF240_22080"
     CDS             <1..982
                     /locus_tag="VF240_22080"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX7177965.1"
                     /translation="PLVVEQFPAGYSNLTYLVRAGAQEFVLRRPPFGSKVRTAHDMGR
                     EYAILTRLHPVYPKTPRALLFCDDESVIGAQFYVMERVRGVVLRARAPEGLALAPEVL
                     RRLSESFVDTLAELHGVDYAAAGLGDLGRPEGYVRRQVEGWTRRYYDAWTEDVQEIET
                     LERWLAANLPADSGAALIHNDYKYDNIVLDPADLARIVAVLDWEMATVGDPLMDLGTT
                     LAYWVEPEDPEEWRQLGFGLTALPGNLTRRELAERYARARGRDGELGPLVFYYAYGLL
                     KIATIVQQIYYRYRKGHTRDARFAALAPLVKACGSIALRAIERGRIDDLG"
     gene            complement(1262..3202)
                     /locus_tag="VF240_22085"
     CDS             complement(1262..3202)
                     /locus_tag="VF240_22085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX7177966.1"
                     /translation="MKSIHVTLALAATLALAAATTRAQQPSATPPQGAEAVRARYTKY
                     EHQIPMRDGVRLFTQVYVPKDTSQRYPFMFLRTPYSVSPYGVDSYRNSLGPSEHFERE
                     GFIFVYQDARGRYMSEGTFEQVRPHVPTKRGPKDIDESTDAYDTIEWLLKNVANHNGR
                     VGMWGVSQPGFHVAASMIDSHPALRAASPQAPTADYYMGDDVYHNGAFMLAANFDFYS
                     GFVPRKGDPTPPRPRVGFDPGTPDAYDFLLGVGPLSTVNQRLFGGEAKYWQEIIDHTT
                     YDDFWKRRSLWRFMNNVSAAVLNVGGWFDAEDPVGAFHIYRAVERQSPGAVNLLVMGP
                     WSHGGWARGGGERLGNVPFAVRTGEFFREHIQFPFFMRHLKDKPGELPEAFMFLTGIN
                     EWRRLPTWPPSGVRPLTLHLGGDGRLTTTAPAAAGQSAFDEYVSDPNRPVPFVGYVAE
                     GMTRDYMTEDQRFASQRPDVLTYVSEPLEEDLLIAGPIKVGLNVSTTGTDADFVVKLV
                     DVYPGDYPTPRAPEGQAPPSNAVRMGGYQQLVRGEPFRGKYRRSFERPEAFTPGRPEA
                     INFEMPDVYHAFRRGHRVMLHVQSSWFPLVDRNPQKFMEIPKATAADFQKATHRVYRS
                     RALNSSITLMVEGNTPRPLVPR"
BASE COUNT          538 a         1025 c         1172 g          564 t
ORIGIN      
        1 gccgctcgtg gtcgaacagt ttccggccgg ctactcgaac ctcacttatc tggtgcgcgc
       61 cggggcgcag gagttcgtgc tgcgccggcc gccgttcggc tcgaaggtca ggacggcgca
      121 cgacatgggg cgcgagtacg cgatcctcac gcgcctgcat cccgtctatc cgaagacgcc
      181 gcgcgcgctc ctgttctgcg acgacgagtc ggtcatcggc gcacagttct acgtgatgga
      241 gcgcgtgcgc ggcgtcgtcc tgcgcgcgcg tgcgcccgag gggctggcgc ttgcgccgga
      301 ggtgctgcgg cgtctctccg agtcgttcgt cgatacgctg gccgagttgc acggagtcga
      361 ttacgcggcg gccgggctgg gagatttggg acggcccgaa gggtacgtca ggcggcaggt
      421 cgaaggatgg acgcgcaggt attacgacgc gtggacggag gacgtgcagg agatcgagac
      481 ccttgagcgc tggctcgccg cgaacctgcc ggccgactcg ggcgcggcgc tgattcacaa
      541 cgattacaag tacgacaaca tcgtgctcga ccccgcagac ctcgcgcgca tcgtcgccgt
      601 gctcgactgg gagatggcca ccgtcggcga cccgctcatg gatttgggca cgacgctcgc
      661 ctactgggtg gagccggaag accccgagga gtggcggcag ttaggcttcg gcctcacggc
      721 gctccccgga aatctgacgc ggcgcgaact ggccgaaagg tacgcgcgcg cgcgcgggcg
      781 cgacggagaa ctcggcccgc tggtcttcta ctacgcctac ggtctgctca agatcgccac
      841 catcgtccag cagatttact accgctaccg caagggccac acgcgcgacg cgcgcttcgc
      901 cgccctcgcc ccgctcgtca aggcgtgcgg cagcattgcc ctgcgcgcga ttgagcgcgg
      961 gcgaatcgac gacctcggtt aacgtcaatg ggcggcgtca accagccccg gaggggcggc
     1021 agaatgtagc cccggtcgtg agaccggggt caacgcggaa caaaatcgac gaagccccgg
     1081 aggggcgaaa gaagagcggg cttgtccatt atgcccgcca catttctttc gcccctccgg
     1141 ggctttcgga tttttatcgc aacctgttcc cacggctcac gccgtgggct acattctgcc
     1201 gccgcctccg gcggctagga tttgcttgct gacctgatgg cgctcgacgg ggcgtcagtc
     1261 ttcagcgcgg cacgagcggg cgcggcgtgt tgccttcgac catgagcgtg atggaagaat
     1321 tgagtgcgcg cgagcggtag acgcggtgcg tggccttctg gaagtcggcg gccgtcgcct
     1381 tcgggatttc cataaacttc tgcgggttgc gatccacgag cgggaaccaa ctgctctgga
     1441 cgtgcagcat cacgcggtgc ccgcgccgga aggcgtggta cacgtccggc atctcgaaat
     1501 ttatcgcctc ggggcgtccc ggcgtaaacg cctcgggccg ctcgaagctt cggcgatact
     1561 tgccgcggaa gggttcgccg cgcacgagct gctggtagcc gcccatcctg acggcgttcg
     1621 acggtggggc ctggccctcg ggcgcgcggg gcgtcgggta gtcgccgggg tacacgtcca
     1681 cgagcttgac gacgaagtcg gcgtccgtcc ccgtcgtcga gacgttgagg ccaaccttga
     1741 tggggccggc gatgaggagg tcttcctcca gaggctcgga gacgtatgtg agcacgtcgg
     1801 gtcgctgcga ggcgaagcgt tgatcctcgg tcatgtagtc gcgcgtcatg ccctcggcga
     1861 cgtagccgac gaaggggacg gggcggttgg ggtcgctcac gtattcgtcg aaggcgctct
     1921 gcccggctgc cgccggcgcg gtcgtcgtca aacggccatc cccacccaaa tgaagcgtca
     1981 gcggcctcac acccgaaggg ggccatgtgg ggagccttcg ccactcgttg atgccggtga
     2041 ggaacatgaa cgcctcgggc agctcaccgg gcttgtcctt gaggtggcgc atgaagaagg
     2101 ggaactgaat gtgttcgcgg aagaactcgc cggtgcggac ggcgaagggg acgttgccga
     2161 ggcgttcgcc gccgccgcgc gcccagccgc cgtgcgacca cggccccatc acgaggagat
     2221 tgaccgcgcc gggactttgg cgctcgacgg cgcggtagat gtggaacgcg cccacggggt
     2281 cttcggcgtc gaaccatccg ccgacgttta agacggccgc gctcacgttg ttcataaagc
     2341 gccacagcga gcgccgcttc cagaagtcgt cgtaggtggt gtggtcgatg atctcctgcc
     2401 agtacttcgc ctcgccgccg aacagacgct ggttgacggt tgagagcggg ccgacgccca
     2461 agaggaaatc gtaggcgtcg ggcgtgccgg ggtcgaagcc gacgcgtgga cggggcggcg
     2521 tggggtcgcc cttgcggggg acgaagccgg agtagaagtc gaagttggcg gcgagcatga
     2581 aagcgccgtt gtggtacacg tcgtcgccca tgtagtagtc ggccgtgggc gcttgcggcg
     2641 aggcggcacg gagtgccggg tgcgagtcga tcatgctggc ggcgacgtgg aagcccggct
     2701 gcgagacgcc ccacatcccg acgcgcccgt tgtggtttgc gacgttcttt aaaagccact
     2761 cgatggtgtc gtaggcgtcc gtgctctcgt cgatgtcttt ggggccgcgc ttcgtgggga
     2821 cgtgcgggcg cacctgctca aacgtgccct cggacatgta gcgtccgcgc gcgtcctgat
     2881 agacgaagat gaagccctcg cgctcgaagt gctccgaagg cccgagcgag ttgcggtaac
     2941 tgtccacgcc gtagggcgag acgctgtagg gcgtgcgtag gaacatgaac gggtaacgct
     3001 gcgaagtgtc tttcggcacg tagacctgcg tgaaaagacg gacgccgtcg cgcatcggaa
     3061 tctgatgctc atacttcgtg tagcgcgcac ggacggcctc cgcgccctgc ggcggtgtag
     3121 cggacggctg ctgcgcgcgc gtcgtcgcgg cggcgagggc gagcgtcgcc gcgagggcga
     3181 gcgtcacatg gatgcttttc atcaaacgaa cctttaacac gttcggtctc cttcagagtg
     3241 caggggcaga agcccgaccg tcagggaagg cgtacacacg ccgaagtacg ccctccctg
//
LOCUS       DASVES010000518         7919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_841032, whole genome shotgun sequence.
ACCESSION   DASVES010000518 DASVES010000000
VERSION     DASVES010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841032"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VF240_22090"
     CDS             <1..737
                     /locus_tag="VF240_22090"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7177967.1"
                     /translation="TMVTGPKIKTFDLALEGYGQGYAGQMTPFQMALAAATIGNLEGK
                     LMKPKMEYDLAPVVFNQVITPQHAAEMRRVMGLVTGGASGTARGVFGPVHAAGIITGG
                     KTGTAQKDVPLYDPKTGEVRTVKKQEKDRRGNVIREYEEVLMAPEPRIDGWFLCIAPL
                     ERPQIAMAVIIEGGGYGGRSAAPVAAALVLKARELGLLGLAPAPAAAAQRQLPQPGAS
                     PRPASPGAKPSARRPAARPNPRPTGQ"
     gene            827..2533
                     /locus_tag="VF240_22095"
     CDS             827..2533
                     /locus_tag="VF240_22095"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX7177968.1"
                     /translation="MKNRASSHLLVLLLIIAFEVVGYVAVYESALLRGYVPSLVQAGR
                     DLLLYVPIIALVFWLSRSMRFAGNWTLFTAAILLFSVGMLVQFRLYSDPEYGSRQKAE
                     ARAEKTQTLRMRYIGQFYDAEKKKRLGLPENYSAEDALKAETAVNQNQYTLTQALTSS
                     ATWIPIIAFISCGVAFRFCTRDDFLAWVQRNSFLIVLITLVPLGAAVASSSAGKALGN
                     MTPWEPSKIPFLLGFAGILTTHYRELTQTYWGLPRARNVVPLLAMAAIPFIPFFALKD
                     FGQMLVFSSAYATLYLVAVRRWPQILLFVGSVALVIAVLVVGALPRDTQERVPSLATL
                     AAPVRAVLPDRIKQRFHLWLDGFNPPSPSEAWWKKDYDDALARDEKMKRLAEESPGMQ
                     RTVNVDVWFDRVAFQPAQATFGVAAGGTTGRGLGLGFSEVIPIADSDYIYAAIAEELG
                     LMGGAVVMLALIVFVNAGIRTALDSRDMFTKLCSAGLAAFIGFQALVNIGGITRTLPM
                     TGITLPFVSHGGFSLITSFAMLGMLMAFSHRNARDTLAPPPRPRANTHPEEEPVPLEA
                     VQ"
     gene            2530..4137
                     /locus_tag="VF240_22100"
     CDS             2530..4137
                     /locus_tag="VF240_22100"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX7177969.1"
                     /translation="MTRQTTDVKIQAAAVTDRGLNERRPLNEDSYLSEVERGIFVVAD
                     GVGGAEAGEVASQTAVEVLGEAFRQQRDDEDVEDLMEIAIQRANDAIHRQAREDKRFS
                     MMATTIVVLHLHGLRATVGHVGDSRIYRITPDGSIRRETEDHSVVEEEVRAGRMTPEQ
                     AQHHPSRNVISRALGAEPDVEVDLNTFEVESGTTFLLCTDGITRHIDDRELARIVSGA
                     ANLDAACQEMKRLCFERGAEDNLTAVLVRVGDAPTQRLAPAAADDGWDEEQTLISGPK
                     PEADTSAAAAAVPAPPAARAPAVEDRAPDTSSAALRNFGRADDDDDDDGWRDPRPKLS
                     PPPPTRPVAAAKKGVGAGIVFFMLLLVLAFSGAAFYAGMFYEGQRRPASNSNVAASPT
                     PAQSARATGAAVTFEAERGRVDSSFFTEAERRASDLAAQPSKADDPEFLYFYGRALLL
                     SNRAPDAAVQFEKAMGIIRQQGKSSQMSLLLDAQMANATAQLRSNNLDGAKKTADSLG
                     QLYPAEGGATPAPSPTPTLAPTPVAQF"
     gene            4196..5794
                     /locus_tag="VF240_22105"
     CDS             4196..5794
                     /locus_tag="VF240_22105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX7177970.1"
                     /translation="MSELKLQQCRVDGRYDVLDCLGRGSYSEVYVARDVAAADGAPAM
                     IVLKALNTYLQGAPDPELEQTLIDNFRNEAVALDRVRHPNVINRLGHGTAIDLTGRVF
                     HYLVLEYLPGGDLNALCRRRPLKLEGALFYLEQVCSGMEHAHSRGVIHRDIKPQNLLL
                     TADQHTVKIADFGVAKLGATDGAITRVGTDVYAAPEHHPLMQTGQLDTASLTMGALHL
                     TPAADIYSLAKTAYALLVGEPPRRFSQRPITELPAKFMAEPWGWEVARVLERATQSRP
                     EKRYQAVGDFWADLREASLPHTRLLGQSNGPVFAPEAEGLNEEDADGTGGTPPPPPAP
                     VFTEKTGPAPAVADRSKDSQHFRIVVPISTASATREAAPPARRQPAEPPARRREEASA
                     HASLRPEPASTHPRRRGLSDRAKKWLVALFLIAAFAGMLLATYFYFRREPRASQTPTA
                     PGAQAGRESPVGREFVATNDVNLRGQPNGQLVGKVPNGSVVKVQAVNGGWYQIQILQY
                     KRPKERASDADQGWADKDFFTERQ"
     gene            5847..6686
                     /locus_tag="VF240_22110"
     CDS             5847..6686
                     /locus_tag="VF240_22110"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEX7177971.1"
                     /translation="MLDSIRKWIDGEGAEDPLAHVDEQARPRRVWEDFLVKVAREVEV
                     VMQRELFTPPGGPTYIPPEYVVYLSSDDDREWQGEKRRGLEQGLYFVLSERARELSGT
                     TQLATKSFAVELRVDGTLEKGQFRVQPVWDTVESGSTMVTARPAELSQAAAETEQGGE
                     DDMTEGEVTRVTARPQELYKIEVWRNNVRQSVVPVMKPEITIGRGSKSVAVDFPVKGD
                     PEVSRLHAVLARDPDGRFWLTPKGRNATLIGGREMARDERIEVRPDDKIELASFVLRI
                     QPG"
     gene            complement(6961..7650)
                     /locus_tag="VF240_22115"
     CDS             complement(6961..7650)
                     /locus_tag="VF240_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177972.1"
                     /translation="MRKMFIAIVPVIWALLVVTSPPATAATGLAPSVAQSALNSRSLN
                     SIEAYAKRLEAFKKRNPNRARIFGNLISINQETYPIPHDAPRSWEEFPTRKARESAAL
                     KRPDITAYDGAEVWIRNGKVVLVRVEPDRYFHGGARIVTYYFRDDGSLAKIRVEFGID
                     GIDGEVVKGTFYDAKGSVLQTRMQCMSTERRRRRIVNCSTMLSQSDDDAPPVYRRVEE
                     LPFINVLKDNS"
BASE COUNT         1393 a         2758 c         2450 g         1318 t
ORIGIN      
        1 cgacgatggt cacggggccg aagatcaaga cgttcgatct cgcgctcgaa ggctacggac
       61 agggctacgc cgggcagatg acgccgtttc agatggcgct cgccgccgcg accatcggca
      121 acctcgaagg caagctgatg aagccgaaga tggagtatga cctcgcgccc gtcgtgttca
      181 atcaggtcat cacgccgcag cacgcggccg agatgcgccg cgtcatgggg ctggtgacgg
      241 gcggcgcgtc gggcacggcg cgcggcgtct tcggccccgt ccacgccgcc ggaatcatca
      301 cgggcggcaa aacgggcacg gcgcagaagg acgtgcccct ctacgacccg aagacgggcg
      361 aggtccgaac cgtcaagaag caggagaagg acaggcgcgg caacgtcatc cgcgagtacg
      421 aagaggtgtt gatggcgccc gagccgcgca tcgacggctg gttcctctgc atcgccccac
      481 tggagcggcc gcagatcgcc atggccgtca tcatcgaggg cggcggctac ggcggacgct
      541 cggccgcgcc cgtcgcggcg gcgctcgttc tcaaggcgcg cgagctgggc ctcctcggcc
      601 tcgcccccgc cccggccgca gctgcccaga gacagcttcc ccaacccggc gcctcccccc
      661 ggccggcgtc gcccggcgcg aaaccctcgg cccgtcggcc ggcggcgagg cccaaccccc
      721 ggccaacggg acagtaattt gaagaggtta gaggttagag gtcagaggtc ggtagaaaga
      781 ccttctttaa ctaacctcta gcctctaacc tctgacctct tgacttatga aaaaccgcgc
      841 gtcatcacat ctgctggtct tgctcctcat catcgccttc gaggtggtgg gctacgtggc
      901 ggtctacgag tccgcgctgc tgcgcggtta cgtgccgtcg ctcgtccagg cggggcgcga
      961 cctactgctc tacgtgccca tcatcgcgct cgtcttctgg ctctcgcgct cgatgcgctt
     1021 cgcgggcaac tggacgctct ttacggccgc catccttctc ttctccgtcg gcatgctcgt
     1081 gcagtttcga ctctacagcg accccgagta cggctcgcgg cagaaggccg aggcccgcgc
     1141 cgagaagacg cagacgctcc gcatgcgcta catcggacag ttctacgacg ccgagaagaa
     1201 gaagcgtctg gggctgcccg agaactattc cgccgaggac gcgctcaagg ccgagacggc
     1261 cgtcaatcaa aatcagtaca cgctcacgca ggcgctcacg tcgagcgcaa cgtggattcc
     1321 catcatcgcc ttcatctcct gcggcgtcgc cttccgcttc tgcacgcgcg acgactttct
     1381 cgcatgggtg cagcgcaaca gctttctcat cgtgctcatc acgctcgtcc cgctgggcgc
     1441 ggccgtcgca agctccagcg ccggcaaggc gctcggcaac atgacgccgt gggagccgtc
     1501 gaaaattccc ttcctcctcg gcttcgccgg catcctcacg acgcactacc gcgagctgac
     1561 gcagacctac tggggactgc cgcgcgcccg caacgtcgtc cccctgctcg ccatggcggc
     1621 catccccttc atccccttct tcgcactcaa agacttcggg cagatgctcg tcttcagtag
     1681 cgcctatgcc acgctctatc tcgtcgccgt gcgccgctgg ccgcagatac ttctgttcgt
     1741 cggctcggtc gcccttgtca tcgccgtctt agtcgtcggc gcgctgccgc gcgacacgca
     1801 ggagcgcgtg ccgtcgctcg ccaccctggc cgcgcccgtg cgcgccgtgc tgcctgatcg
     1861 catcaagcaa cggttccacc tctggcttga cggattcaac ccgccgtcgc cctctgaggc
     1921 gtggtggaag aaagactacg acgacgcgct cgcccgcgat gagaagatga agcggctcgc
     1981 cgaggaaagc cccggcatgc agcgcaccgt caacgttgac gtgtggttcg accgcgtcgc
     2041 cttccagccc gcgcaggcca ccttcggcgt cgccgccggc ggcaccacgg ggcggggact
     2101 cggcctcggc ttctccgaag tcattcccat cgccgactcc gactacattt acgccgccat
     2161 cgccgaagag ctgggcctca tgggcggcgc cgtcgtcatg ctcgcgctca tcgtcttcgt
     2221 caacgccggc atccgcacgg ccctcgattc gcgcgacatg ttcacgaagc tctgctcggc
     2281 ggggctggcg gccttcatcg gcttccaggc gctcgtcaac atcggcggta tcacgcgcac
     2341 gctgccgatg accggcatca cgctcccctt cgtcagccac ggcggcttca gcctcatcac
     2401 cagcttcgcc atgctcggaa tgctcatggc cttctcgcac cgcaacgccc gcgacaccct
     2461 cgcaccgccg cctcgcccgc gtgcgaacac gcacccggag gaggagccag tcccgctgga
     2521 ggccgtccaa tgacgcgaca aactaccgac gtcaaaatcc aggcagcggc cgtcaccgac
     2581 cgtggcttga acgagcgccg cccgctcaat gaggactctt acctgtcgga ggtggagcgg
     2641 ggaatctttg tcgtggccga cggcgtcggc ggggcggagg ccggcgaggt cgcctcgcaa
     2701 acggccgtcg aggttctggg cgaggcgttt cggcagcagc gcgacgacga agacgtggaa
     2761 gacctgatgg agatcgccat ccagcgtgcc aacgacgcca tccaccggca ggcgcgcgag
     2821 gacaagcggt tctcgatgat ggcgacgacc atcgtcgtcc ttcacctcca cggattgcgc
     2881 gccacggtcg gccacgtcgg cgactcacgc atctaccgca tcacgcccga cggctcgatc
     2941 cgccgcgaga ccgaagacca ctcggtcgtc gaggaagagg tgcgcgccgg gcggatgacg
     3001 cccgagcagg cccagcatca tccgagccgc aacgtcatca gccgcgccct cggcgccgag
     3061 cctgacgtgg aggtcgatct caacaccttc gaggtcgaga gcggcaccac cttcctgctc
     3121 tgcaccgacg gcatcacgcg tcacatcgac gaccgcgagc ttgcccgtat cgtctccggc
     3181 gccgcaaatc tcgacgccgc ctgtcaggag atgaagcgtc tgtgcttcga gcgcggcgcc
     3241 gaggacaatt tgacggccgt gctcgtccgc gtcggcgacg ccccgacgca gcgcctcgcc
     3301 ccggccgcgg ccgacgacgg ctgggacgag gaacagaccc tcatctccgg gccgaagccc
     3361 gaggccgaca cctccgccgc cgccgccgct gtccccgccc cccccgccgc gcgtgctccg
     3421 gccgtcgaag accgcgcgcc agacacctcc tcggccgccc tccgcaactt cggacgcgcc
     3481 gatgacgacg atgacgacga cggctggcga gacccgcgac ccaagctctc gcccccgccg
     3541 cccacgcgcc ccgtcgccgc cgcaaagaag ggcgtgggcg cagggattgt cttcttcatg
     3601 ctcctgctcg tcctcgcctt ctcgggcgcg gccttctacg ccgggatgtt ctacgagggg
     3661 cagcgccggc cggcgtccaa ctctaacgtc gccgcttcgc ccacgcccgc gcaaagcgcg
     3721 agggcgacgg gcgcggccgt cactttcgag gccgagcggg gccgcgtcga tagctcattc
     3781 ttcacggagg ccgagcggcg cgctagcgat ctcgccgcgc agccgtcgaa ggccgacgac
     3841 ccggagttcc tctacttcta cggccgcgcg ctcctgctct cgaaccgcgc gcccgacgcg
     3901 gccgtacagt ttgagaaggc catgggcatc atccggcagc agggcaagtc gtcgcagatg
     3961 tcgttgctcc tcgacgcgca gatggccaac gccacggcgc aactgcgctc gaacaatctc
     4021 gatggcgcga agaagacggc cgactctttg ggccagctct acccggccga ggggggcgcg
     4081 acaccggccc cctccccgac tccgactctg gctcccacgc cggtggcgca gttttgaagt
     4141 ggcgcggcgg tgatcgttat ttgtttgacg cgcggccgaa gacgccgaaa caggtatgag
     4201 tgaactgaag ctccagcagt gccgggtcga tggccgttac gacgtgctcg actgcctggg
     4261 gcgcggcagc tattccgagg tctacgtggc gcgcgacgtg gcggccgccg acggcgcgcc
     4321 cgcgatgatc gtcctcaagg ctctcaacac ctatcttcag ggcgcgcccg accccgagct
     4381 ggaacagacg ctcatcgaca acttccgcaa cgaggccgtc gcgctcgacc gcgtgcgcca
     4441 ccccaacgtc atcaaccgtc tcggccacgg cacggccatc gacctcacgg gccgcgtctt
     4501 ccactatctc gttctcgaat acctgcccgg cggcgacctg aatgcgcttt gccgccgccg
     4561 cccgctcaag ctcgaaggcg ctctcttcta tctcgaacag gtctgctcgg ggatggagca
     4621 cgcacactcg cgcggcgtca tccaccgcga catcaagccg cagaacctcc tcttaacggc
     4681 cgaccagcac acggtgaaaa tcgccgactt cggcgtcgcc aagctggggg ccaccgacgg
     4741 cgccatcacg cgcgtcggca cggatgtcta cgccgcgccc gagcaccacc cgctgatgca
     4801 gaccgggcag ctcgacacgg cctcgctcac aatgggcgca cttcacctga cgcccgcggc
     4861 cgacatctac tcgctcgcca agaccgccta cgccctcctc gtcggcgagc cgccgcgacg
     4921 tttctcgcaa cgaccgatca ccgagctgcc cgcgaagttc atggctgagc cgtggggctg
     4981 ggaggtggcg cgcgtgcttg agcgcgcgac gcagagccgg ccggagaagc gttatcaggc
     5041 cgtgggtgat ttctgggccg acctgcgtga ggcgtcgctg ccgcacacga ggctgttggg
     5101 gcaatcgaac gggccggtct ttgctcccga ggcggagggg ttgaatgagg aggacgctga
     5161 cggaacgggc ggcacgccgc cgccccctcc tgcgccggtc ttcaccgaaa agacgggacc
     5221 ggccccggcc gtcgccgaca ggtcaaaaga ctcgcagcac tttcgcatcg tcgtccccat
     5281 ctcgacggcg tcggccacgc gcgaggccgc gccgccggcc cgccggcaac ccgcggagcc
     5341 accggcacgc cgacgtgaag aggcgagcgc gcacgcgtcg ctccgacccg agcctgcgtc
     5401 cacgcacccg cgccgaaggg gactctccga ccgcgcgaag aaatggctcg tggcgctctt
     5461 cctcatcgcc gcattcgcgg ggatgctcct cgccacatac ttctatttcc gccgcgagcc
     5521 gcgcgcgagc cagacgccca ccgcgcccgg cgcgcaggcc gggcgcgagt cgcccgtcgg
     5581 gcgtgagttc gtggcgacga acgatgtaaa cttgcgcggc cagccgaacg gacaactcgt
     5641 gggcaaagtt cctaacggat cggtcgtcaa agtccaggcc gtgaacggcg gctggtatca
     5701 gattcagatt cttcagtaca agcggccgaa ggaaagggcg tcggacgccg accagggctg
     5761 ggcggacaaa gatttcttca cggagcggca gtagcacgaa agcgcggccg ccccttccat
     5821 tattgagttg ggagaaaaaa gtgggattgc ttgattcaat caggaagtgg atcgacggcg
     5881 aaggcgctga agacccgctg gcgcacgtgg acgagcaggc gcggccgcgt cgcgtgtggg
     5941 aggatttcct cgtcaaggtg gcgcgcgagg tcgaggtcgt gatgcagcgc gagctgttca
     6001 cgccgcccgg cggccccacc tacattccac ccgagtacgt cgtctatctt tcgagcgacg
     6061 acgaccgcga gtggcagggc gagaagcggc gcgggctgga gcagggcctc tacttcgtcc
     6121 tctccgagcg ggcgcgtgag ttgtcaggca cgacacagct tgcgacgaaa tcgttcgccg
     6181 tcgagctgcg cgtggacggc acactggaga aggggcagtt ccgcgtgcaa cccgtgtggg
     6241 acacggtcga gagcgggagc acgatggtga cggcgcggcc cgccgagctt tcgcaggccg
     6301 cggccgaaac ggagcagggc ggcgaagacg acatgacgga gggcgaggtg acgcgagtca
     6361 cggcgcgccc gcaggagctg tataagattg aggtctggcg caacaacgtg cggcagtcgg
     6421 tcgtccccgt gatgaagccg gagatcacca tcgggcgcgg ctcgaagtcg gtcgccgtgg
     6481 acttccccgt caagggcgac cccgaggtca gccgcctcca cgccgtgctc gcgcgcgacc
     6541 ccgacgggcg cttctggctc acgcccaaag gccgcaacgc cacgctcatc ggcgggcgcg
     6601 agatggcccg cgacgagcgc atcgaagtcc gccccgacga caagatcgag ctggcgagct
     6661 tcgtcctgcg aatccaaccc ggttaaagcc cggcatcagc ggcgagcgcc cgttcaacgc
     6721 acataaagga aatgctgagg cgcgagtccg ctgcatgccg ggcgttaggc ggcggtcaca
     6781 aaggtatatc gctctcatcg agggcaagtg ctttgacaag ttttaaaggc ttcggcacac
     6841 ctccaaaccc cagggtgttg cgttggcgcg ggcaatggag ttctcgcctc ggcaaaattc
     6901 cagtccttgc catgctcgcg gttcattgcc aacgcgttag gaaaatgcag ggggcggatt
     6961 ttaggagttg tccttcagga cattaatgaa cggcaactct tcaactcgtc ggtagacggg
     7021 aggagcatcg tcatcggatt gagaaagcat cgttgagcag ttcactatcc tgcgcctgcg
     7081 cctctcggta ctcatacact gcatccttgt ctgcaacacg cttcccttgg cgtcgtaaaa
     7141 cgtccccttc acgacctcgc cgtctatgcc gtctatgcca aactcaaccc ggattttcgc
     7201 cagtgaccca tcatctcgaa agtaataggt gacgatccgc gcgcctccgt gaaaataccg
     7261 gtccggctca acccgcacta atactacctt cccattcctt atccacacct cagcgccgtc
     7321 gtaggctgta atatcgggac gcttcagtgc cgcagattct cttgccttac gagttgggaa
     7381 ctcctcccaa gatcgcggtg cgtcgtgggg aatgggatag gtctcctgat ttatagaaat
     7441 aagattgccg aagatccggg ctcgattggg attgcgtttc ttaaatgctt ccaacctctt
     7501 ggcgtaagcc tcaatggagt tgagggatcg agaattcagc gccgactgcg ccacggacgg
     7561 ggctaggccc gttgccgcag tggcaggtgg cgacgtgact accagaagtg cccagataac
     7621 cggcacgatt gcgatgaaca tctttctcat tactcctctc ctctttggtg ggcggatcac
     7681 cctcgttgcc ctttcgaagc tcgatcctct tcaaaaactg cttgtctgtt tctcgcgccc
     7741 aaatgttcgc gagccaaccc tcatcttgga gtgcagtcga tctcaaaaca tttcagcatc
     7801 tcaccgccgc tcgactctca ccagatattg ccccggcgca gccattgcgc cctcttcgct
     7861 cagcaagtcc agtttgaacg tgtaatcgcc ggggttgagc agtcttgctg gaacatgaa
//
LOCUS       DASVES010000519        26021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_495326, whole genome shotgun sequence.
ACCESSION   DASVES010000519 DASVES010000000
VERSION     DASVES010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 26021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26021
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495326"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1387)
                     /locus_tag="VF240_22120"
     CDS             complement(161..1387)
                     /locus_tag="VF240_22120"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX7177973.1"
                     /translation="MGPHDEQRKPVTFFRWVFKRVRQKGAFYWGTVVALIVIGVFVGR
                     WLEKQEFALELRYKLFKMVQSASPNKPFVQGTSVVLIGDEEYWKGDLARRIPIKRDYL
                     AALLRRLDKADPAVIALDFDLRAQTPDGSYVMHPDYQDETKQLLEAVRDVSKNRSVIL
                     PKTMTRLKSGGYVTEPDIYDGFDFQGGKVRTGYIALPFDIRKVPLSQKLRDGGRVESF
                     AEAIVRARNERALDRVRDLESLPYGDYLEHGAFDVFSPADVLANKPEVLDKLRHDIVI
                     VGAGWSNRAYGRGGAADSYFTPVGPLQGALIHANFVEALLGNRTSRPFGETALKVIEV
                     IATLLVAVIFALISNPLGKFFSIVLLMAMLVLFSIFSRLNLGLFYDFFIPMLLVVVHA
                     AYEQIREWQTHAHRVP"
     gene            complement(1788..3497)
                     /locus_tag="VF240_22125"
     CDS             complement(1788..3497)
                     /locus_tag="VF240_22125"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7177974.1"
                     /translation="MKHGRGFDPTTLEIYRALYTSVAEEMGVTLRRTAHSPNIKERRD
                     YSCAVFDARGRVIAQGDHMPVHLGSMPLAVAAALKETTLGPGDVVIVNDPFAGGTHLP
                     DVTLVAGVFDAEGKGDGKRGKGEGVKENGRKTARFAKQNRLSDAPTLTPSPLPLPLFY
                     VANRAHHADIGGATPGSMGIATDVYGEGLRIPPVHLVRGGRVVDDVLRLVLANVRVER
                     ERRADFEAQMGSLRTGAARLLEIVGRSGARESAEYAGHLIAYSARRMRAVIREIPDGT
                     YEAADALDDDGIVAGTEVTLRVSVRIRGERARVDFTGSAPQVAGPINAVEAITVSAVS
                     YVFRCLIGAEEVPASAGLMEPIEVVAPAGTVVNANPPASVAGGNVETSQRIVDVLFMA
                     LAKALPGRIPAASQGTMNNLTVGGTEARTGEEFAYYETVAGGMGARAARDGVGGVHTH
                     MTNSLNTPAEALEYAYPLRVRTYALRKGSGGRGARNGGDGVVREIETLVPARMSLLAD
                     RRRRGPYGLDGGADGKPGRDSIIRRGRARKIDAKGSRELEAGDRVRIETPGGGGHGED
                     EKQ"
     gene            complement(3494..5536)
                     /locus_tag="VF240_22130"
     CDS             complement(3494..5536)
                     /locus_tag="VF240_22130"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7177975.1"
                     /translation="MKGRKDTRRVRVGVDTGGTFTDFVYEANGAAKIFKLASTPDDPS
                     RAISDGLRRVAREAGVSLRDTQVVHGTTVGTNALLQRRGARTALVTNAGFEDVLVIGR
                     QARGSLYDLDWERPAPLVPDELRLGVGGRIAPDGAVVEELDEAALAALVRKLKRARVE
                     SVAVSLLFSFANPAHERKVESALADGLKDVALSVSHKILPEYREYERTSTVAINAYLQ
                     PLMGAYLRSVGSSVPALRVMQSSGGSISAVAAAREPVRTILSGPAGGVVGALSCARAA
                     GLSDIITFDMGGTSTDVALCAGGRLRLTNEASVAGLPVAVPVLDIHTVGAGGGSVARV
                     DAGGSLRVGPESAGADPGPACYGRSLLPTVTDANLVLGRFGGGGLLGGEFALDARRAE
                     EALTKLGREMASAGGRRVTATGAALGVLRVVNAGMERALRVVSVERGHDPRAYSLVTF
                     GGAGGLHAVELARALRIPRVVVPADPGALSALGALQSDVVKDLSRTVMLDAGDAKTTA
                     RLERAFTEMEREARAVLRREGFGDDEQRHERTLTARYKGQSFELEIEWKRSGEAALIA
                     RFHHAHEARYGYAQGGRAVEIVSARVRSRGLVEGPRAARMSRRRSSVKTIEPHAVALV
                     HFETGRKRVAVYARAELPAGARLRDPCLITEYSSTTLVPMGARASVDSRGNLIIEP"
     gene            complement(5533..6111)
                     /locus_tag="VF240_22135"
     CDS             complement(5533..6111)
                     /locus_tag="VF240_22135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7177976.1"
                     /translation="MPVSTNEEITYREATSADSRAVAVVNVRSWQESFRGVVPQAHLD
                     GMNYEKRAAAFAERFEQRERARVADGGADFYQMIVAERAGASAVGYVDFGPPRETRFG
                     PFAAELYSIYVLPEQQRRGVGGRLFRLTADALLARGRRSMYLQTLEASPYRNFYERMG
                     GRPVGRVTLQIGGVDFVELAYGWDDLSETLPK"
     gene            complement(6160..7263)
                     /locus_tag="VF240_22140"
     CDS             complement(6160..7263)
                     /locus_tag="VF240_22140"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7177977.1"
                     /translation="MTTDNRIGIGIIGAGFARTTQIPAFRACEGARVVAIASRRRESA
                     GAVASEFDIPFATADWRELLTRDDVDLLSIVTPPATHAEMTLAALSAGKAVLCEKPTA
                     MNFEEAARMRDAAERAGLLAHIDHELRFLPARQLMREMISNGEVGEVWHARVHFRAGS
                     RADTGRAWDWWSDAEQGGGVLGAIGSHAVDSLQWLTDARTTQVSCTLATHVSERPEEK
                     TGELKRVTTDDEATLLLRLGGGAVAEGATAAVSASVVESGESMHVTEVFGPRGALRTD
                     TTGALFRADAAGREWRRVEVDLAPLASGMNDNEWSRGFTEFSRRIVEALRGEGRQARV
                     REAATFDDGYHIQRVLDAARDSHESGCRVTVTD"
     gene            complement(7439..10012)
                     /locus_tag="VF240_22145"
     CDS             complement(7439..10012)
                     /locus_tag="VF240_22145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HEX7177978.1"
                     /translation="MSSKTTRNTPYRFITWLLSVALIAAFISIPAGAQQEDSSEADSL
                     NELQEVGGEESSDTGGDDSPEGKFRRRGGDNFKSPRAADRYQRRQEALKQKLAGKKSN
                     GKVHEVAKGQFVELGLERNDRVFVVLVEYGTQASTNAAVPTTPGPLHNQIAEPNRALN
                     NNTIWQADYSPAHFYDMYFNRMVNYYQTQSSGRYTINGAVTEWVKVPFNGPRYGSNLM
                     GDAGAWTMIADAINVWTKDQLASGKTLDEIKTHLQSFDVWDRYDYNNNGNFDEPDGYI
                     DHFQIVHSGAGEETGGGAQGSNAIWSHRWQAWYNFAGVTGPSYNKDGGVEFGGGWGAN
                     PNGGTTSSSGAVVQGINSSSVNAANVQNAHPLSKTGIWVGDYTVQPENGGLGVFAHEY
                     GHDLGLPDHYDTNGGSNSTGFWTIMSSGSYLGDGTIDIGSRPGDFGGWDKLQLGWLNY
                     DIATTGVPSDHKLGPAETNTKKAQAVVVSLPADKNVFYLFDPTAGKLQYGTKAWWGGK
                     ADNLDTNMVRQVEVPAGTSALTMRLQYNIEANWDYAYVSVSTNNGATWTNLAGTYLNG
                     AVQSALTTNTNANGQNLGNGITGSSAGAWRLASFNTTAYAGQTVLLRLRYKTDAFTVL
                     NGFLADEITLGAFSDGAEAGDNGWTLGGFKATTGIESSNAAHYYIAEYRQYRTYDQGL
                     QTGPYTFGHALTRPSWVTHYPYQNGMLITYWDQDQANDNTSQHRGEGLALPIDAHPEP
                     LRRVVPYDGLNWTFSPWSATIQSFDSTFGLEPTDALTLPFTGTLPPAAGSPAGTPNRV
                     VEFTVSHPSLPAVPVFNDLNSYWSADTPAAGVIVPKTGTIIRVVNTSAQNSMMQVHVG
                     P"
     gene            complement(10464..10539)
                     /locus_tag="VF240_22150"
     tRNA            complement(10464..10539)
                     /locus_tag="VF240_22150"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10504..10506),aa:Ile,seq:cat)
     gene            complement(10981..11625)
                     /locus_tag="VF240_22155"
     CDS             complement(10981..11625)
                     /locus_tag="VF240_22155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177979.1"
                     /translation="MMALKTSSSIVIRLLAIMVIIFLSYTILSDMGLFNQRVREVSPV
                     GVSLNSVGAKVEAPLASHTEEVIGTYGFSDLLKSSSDAADSTFAPKVERGENGALTRE
                     RPRQKQKAPPSEQDENSTVLAATSHPSPASPPPVPPPAITKPLNNEESRASEDEAVVV
                     TKRVENGRVVEAYVSNPQPGREALENSALRVVRQRRYQSGVTVTETERISIKKQ"
     gene            complement(11835..13604)
                     /locus_tag="VF240_22160"
     CDS             complement(11835..13604)
                     /locus_tag="VF240_22160"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7177980.1"
                     /translation="MLGPIRLLVLTLSYTAAFHSAEAQEKTAAPASATVVSSDNISRQ
                     EDGLMGPVRRVRKEIAKIIVKSGGLVEGPRKLQGTDLYDRRGKKADNEYFTTEEDNLT
                     GKEVYRKFDDKGNVIEMDLQANDGSVISRETYAYEFDAVGNWTKMTTSVAVIEGGKLS
                     YEPIEVTYRTITYYSTDAVAKAINSGTQSSGNAGASPAKEPTPEESLLHPTTPQAAAA
                     NETAPAAGKTKPPNVMPVATAPPGEQAALSKTTDEAEPAAKKEDTKEDTKEDTAAPTL
                     LVKNVSSEELRRAAVYMPQPVYPIGAATAKVVGKVDVQVIVDEKGEVSSTRVVSGHVL
                     LADAAEQAARRARFSPTQLSNEPARVFGLLSYEFNLEPSAGPPSVTSAPVAEASLVAA
                     PADSERTAESSTIGPAVNTSEAKPAAKSSEEADAKKYAQIGSEYYKFGKYKDAVKAFK
                     EASRLGPDNYEVLFKLGASYLELERDADALNAFKKVVSLKPDAADAHYYIGVSNFNLR
                     RYPEAVKSYKRAVQLRAGSADYHFGLGMAYLGAKDISSARNQVEILKGLNTKLAGVLA
                     ETARAFSHPGFQSGGGFADRMRR"
     gene            complement(13970..15376)
                     /locus_tag="VF240_22165"
     CDS             complement(13970..15376)
                     /locus_tag="VF240_22165"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7177981.1"
                     /translation="MSSTHIPLIRAAAQGDAGAVSALLADGADVNVSTLKGVTALIGA
                     AFFGHADVVRVLLHAGADVRMKDYLGLTATEWSIRRGFDEVTRLLQDTPPAGPKTPGM
                     NAAEEDYRQAAEVVRQQGEEPAEIKGREERQQRANPAQRLGQSTEPLDAQTATARGAH
                     DPQAADALLDLSHVQPAALAADFELDLDSDETPLPAAMRQATPAGQGVAGVASDEDEI
                     TSSVKAAGAIAEEVRERADAEAHQKSEEVARRHYEDVMYGEPTRPASTLGHTAQETPT
                     GSGVRRCPKCNSTYRSDILAYCLYDSVRLISDDEPSVTAAVEPYVSMRDNRNVGRPWA
                     PILIALALLGGGLIGYQLNRSFTNQIPAAPGSAQTAPASVQPDQPVTDGALKGKEIRL
                     PSPEYPEVVKSEGVSGNVTVAVVVNKKGDVISARPLNGHPLLRVAAVKAAREAKFSSE
                     KLAGGAAKVSGTITYNFK"
     gene            15735..17147
                     /locus_tag="VF240_22170"
     CDS             15735..17147
                     /locus_tag="VF240_22170"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HEX7177982.1"
                     /translation="MAAGPVGAAATTPQKSAAMGRAERARLAGRVRSEFLHAWRGYKK
                     YAWGHDDLRPLSKAPHDWYGQTLLLTQVDALDTLVVMGLRAEADETRSYIVEHLSLDR
                     DVYVKNFEIVIRLLGGLLSGYQMSGDRRLLALAEDLGMRLLPAFESPTGIPYRYVNLK
                     TGKVRGNVTNPAEAGTLLVEFGTLARLTGRQIFYDKAKRALLEIYQRRSSVGLIGQRI
                     DVETGRWTHTDSHLSAEIDSYYEYLLKCWLLFDDADCRRMWRESVAAANKYLADEVGT
                     ELWYGHADMWTGRRAATTYGALDAFIPAMLALSGDTGRARRLQASSFKMWNKHGIEPE
                     EINYRTMEVGDAGYPLRPEIVESAYYLHHFTRDPVYLRMGESMFDDFVKYCKTDVAYA
                     SLKSVVTKEKADSMESFLFAETFKYFYLLFAPPSALPFEQVIFNTEAHPIRRTWRAGA
                     RSAGGGDLRGARRRRATGRD"
     gene            complement(17059..17604)
                     /locus_tag="VF240_22175"
     CDS             complement(17059..17604)
                     /locus_tag="VF240_22175"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7177983.1"
                     /translation="MKTTFHNVAEIFEALDESRARLDTTLAGLGVEHEALRDAPERWC
                     VAEVVEHVAIIEKLMSQLVGRLLAKAEADGAPPPGADAGPLVALDEVAERARREKYQA
                     PEMAVPRSGASVSDSLATLRESRAALTALRPRVEAVGASVHTYPHPAFGPLNLYQWLA
                     FVALHEDRHRRQIERLLATSA"
     gene            complement(17704..19062)
                     /locus_tag="VF240_22180"
     CDS             complement(17704..19062)
                     /locus_tag="VF240_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177984.1"
                     /translation="MRFTSVKIYLFVCALSASLVAAACQQAGDAVSVRPKTLRDVPAV
                     RLAFRFEADVAAENLPEQLKGDGAPEKNEAVARDFETRRPEEELLRTVTSPDGQRALA
                     LYATSETTGLDFRMDLYSSEGVFLRNIMPPNMVGSFYEEVAWSSDGQHFAFLGFRNPT
                     PAATPDPGRETTAPPSVAVPDASPGDPAAEAPPVAVPTVAPIIAPVAAFGTEQVYLSD
                     RDGLNIRPLTTRDGLIYFELAWSPDAQLLAALACTEAEWNARKGKGEAPGGRPRLIAL
                     DAQERLLDDRLADAAPVWSPDASKVATAFDKTVAVYDAAGGEPTGANLPLQEALWASS
                     VEFDAKMFKKGGAAANTDAGQQGPEGGGSSSSDSAAGGAVPPVGSVVLNSFNPVVRVA
                     WTEPELLFVQTAYMRYFPNEPVPTVRYERWHVVKLYPQATLVSRLPRRPPSGARRPHV
                     AS"
     gene            complement(19133..19705)
                     /locus_tag="VF240_22185"
     CDS             complement(19133..19705)
                     /locus_tag="VF240_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177985.1"
                     /translation="MEEQMSRRLQSAGPLILLLTLCGVFSAPVNAQEAGKDEREFVSA
                     LPSHVPLKVTVKNLEKVKDLRNEKWYGELEVEVKNIGTRPIYRFGLLLRLPEVKHDGT
                     IYAHQFHYGRGELIDLSVKALPSDTPLLPGESSTFMVEERFVKGWEGYSAERNIPPPK
                     KVEFVLLSMSFGDGTGLVGSDGVFLPRRKN"
     gene            complement(19690..20469)
                     /locus_tag="VF240_22190"
     CDS             complement(19690..20469)
                     /locus_tag="VF240_22190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7177986.1"
                     /translation="MSDNFKSIRFRVADRVARVTFARPPLNIFDIAMMREVDRALTDC
                     MTLRDVVAVVFDAAKGSRAFSAGVSVEEHRAETIYQMLDSFHAIFRTMEQLGKPTVAL
                     VEGAALGGGCELVTACDIVISGTRAHYGQPEIKLGVFPPVAAVLLPRVIGERRARELL
                     LTGELIDAQEAHRLGLVSHLVEEGQLEGKLQEVLARLRELSAPALEMTRRALDTARGR
                     TFDDALARVEDLYLNELMKTEDADEGIRAFIEKRKPAWRNR"
     gene            20622..21494
                     /locus_tag="VF240_22195"
     CDS             20622..21494
                     /locus_tag="VF240_22195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7177987.1"
                     /translation="MTEDGISIRDIVEVEEMRAVEELQMEVWGMPERDIVSVFMLRAT
                     NAAGGVLVGAFDGATLAGFAYGFVGLEDGRAILHSDMLAVLPAYRGRDVGYRLKLAQR
                     ERALARGLRVMTWTFDPLQARNARLNFGKLGVVSARYFVNFYGERSSSFLHSTGTDRL
                     WVTWLLDSPRVRQRAATTARVAFDEWPARARELPALVRVGDNDAPVFEEDADAASQMS
                     ERVLVEIPGDITALEERQPALALEWRLATRRAFTSALAAGYLVEEFFRIERAGRRVGA
                     YLLTAGRTPEDFAK"
     gene            complement(21395..23083)
                     /locus_tag="VF240_22200"
     CDS             complement(21395..23083)
                     /locus_tag="VF240_22200"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX7177988.1"
                     /translation="MPFFFASALFISSQRQQLEIAGTVSAQQSQQSSAASNQAEPELT
                     AIVGATLIDGSGRAPVRDSVVVVRGDRIESAGARRRVRVPAGARRLDARGLVIAPGFI
                     DTHNHSGGGLSTDPTAATQISQGITTVAIGQDGGSEFPVGAYLSKMEREPASVNVLTF
                     VGHATLRTRVMGEDTARAATPEEVRRMASLADEAMREGAFGLSTGLEYETGKGATTEE
                     VVALARMAARHGGIYISHIRDEADLTFEALEEAVRIGRDAGLPVQISHIKLGTAGVWG
                     RAREAVALINRARRRGQDVTADCYPYDAWHSTIRVLVPSGRHDDAGDVARALADVGGA
                     ANVTIVSCRAHPEYEFKTLEEIARNRGVSAVEQYMQIVRDGGASVVCRSMKEEDMRVF
                     YEQPWVMVCSDGGIAGRHPRGAGSYPRVLGRYVREQGWLSLPEAVRKMTSLPAARLKL
                     HDRGLVRAGRRADLVLFDPARVRDRATFQQPQLISEGIERVFVGGVEVWRDGKVTGAR
                     PGRVLRLSQTPPAPRAPRRRASHFAKSSGVLPAVSRYAPTRRPARSILKNSSTR"
     gene            complement(23278..25230)
                     /locus_tag="VF240_22205"
     CDS             complement(23278..25230)
                     /locus_tag="VF240_22205"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7177989.1"
                     /translation="MRHTRTAHALALAASLLLLLAAPSSSSDVSAQATRRQTPALASS
                     TSSTQRQAAPEISYTVSMPRPDTHLLEVEMRLRWTAQQGPSTVDLRMPVWTPGSYLVR
                     EFERHVQDFAAEDSGGRALPWTKVNKNTWRVTTPAGARELRARYRVYSNELTVRTNEL
                     HDRHAFWNNAALLMYPEGHLSAPSTLRVVPHANWKVATGLPASGERNTFRAENFDILY
                     DSPFLVSNFKTLSFDVRGVPHRIVIDGDGGNYDPARMERDVKKVVETTVAMMGDVPYR
                     DYTFLLMLRPRGGGGLEHLNSTALIADPFSFRAEPTYRGFLELVGHEYFHVWNVKLIR
                     PDALGPFDYTGENYTRLLWVAEGITDYYGTLLVRRAGLMTDKDYLEQLARAIEAMQNT
                     PGRQKMSVEEASFDAWIRYYRADENSPNVSISYYDKGGLVGFLLDLEIRKRTAGARSL
                     DDVLRQLYTEFAKKNRNFTPEDFQRVAEQQAGASLEDFFRAYVRGREELNYNAALEAA
                     GLRLDTTGAATPAADARAPAPKPFLGGQIAQDGDRLVVRVVQAGTPAYEQGLYANDQI
                     VAVDGYRASLTSLNARVADKKPGDSIALTVFRHDEIRTVNVKLGSKTEGAYRIVPVSA
                     PTPEQMKTYEAWLGAPFPKPAQAGRE"
     gene            25435..25896
                     /gene="trxA"
                     /locus_tag="VF240_22210"
     CDS             25435..25896
                     /gene="trxA"
                     /locus_tag="VF240_22210"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7177990.1"
                     /translation="MSVVVCANCGTKNRVEEEAARERQPVCGRCGVQLGEEVGTAPAD
                     FGKPETVTDATFAGAVLGASEGPPVLVDFWAAWCGPCVMLAPTLDQLAAESGGRYRVA
                     KLNVDENPATASQFGVRSIPTLIIFKGGREVERLVGAQPRQQIAARLAAHA"
BASE COUNT         4299 a         8223 c         8697 g         4802 t
ORIGIN      
        1 ctcctctgcc ggcgattttg gttggaggcg ttggggtcgc gcgggtcgct ggcgcgctcg
       61 gagagggccg cgggcgcgga gaggaggccg acggcaatga gcaagcaggc tggagttgcg
      121 cgcatcttta aatctcctgt aggccggtgc cggccgctgg tcacggcacc cggtgggcgt
      181 gagtttgcca ctcgcgaatc tgctcatacg cggcgtggac gacgacgagt agcatgggga
      241 tgaagaagtc gtagaacaac ccgaggttca acctggagaa gatgctgaac agcaccagca
      301 tcgccatcag gaggacgatg ctgaagaact tcccgagcgg gttggagatg agcgcgaaga
      361 tgacggccac gagcagggtc gcgatgacct caatgacctt cagggccgtc tccccgaagg
      421 gcctgctggt gcggttgccg aggagggcct cgacgaagtt ggcgtggatg agcgcgcctt
      481 ggagcgggcc gacgggggtg aaataagagt cggccgcgcc gccgcggccg taggcgcgat
      541 tgctccaccc ggcgcccacg atgacgatgt cgtggcgcag tttgtctaac acctccggct
      601 tgttggccag cacgtcggcc ggggagaaca cgtcgaaggc tccgtgttcc aggtagtcgc
      661 cgtatggcag ggactccagg tcgcggactc ggtcgagggc gcgctcgttg cgggcgcgaa
      721 caatcgcctc ggcgaaagac tcgacccggc ccccgtcgcg gagcttctga ctgagcggca
      781 cctttctgat gtcgaagggg agcgcgatgt aacccgtccg caccttgccg ccctgaaagt
      841 cgaagccgtc gtagatgtcc ggctcggtca cgtacccgcc gctcttcagc ctggtcatcg
      901 tcttcggtaa gatgaccgac ctgttcttgg aaacatccct caccgcctcc aggagctgtt
      961 tggtttcgtc ctgatagtcg gggtgcatga cgtagctgcc gtcgggcgtc tgcgcgcgca
     1021 ggtcgaagtc gagcgcgatg acggccgggt cggccttgtc caggcggcgg aggagagcgg
     1081 cgagatagtc acgcttgatg ggaatgcgcc gcgccaggtc gcccttccag tattcttcat
     1141 cgccgatgag gacgacactc gtcccctgca cgaatggctt gttgggcgac gcggactgca
     1201 ccatcttgaa gagcttgtag cggagttcga gtgcgaactc ctgcttttcg agccacctgc
     1261 cgacgaagac gccgatcacg atcagggcca ccactgtgcc ccagtagaag gcgcccttct
     1321 gccgaaccct cttgaagacc caacggaaaa aagtgacggg tttgcgctgc tcgtcgtgag
     1381 gccccatgcc gtgactcctt gagtcacctg ccagtcgcag gcgcggatgt tttagagggc
     1441 tgccggggaa cgcattcccc gaccgttggt gatggtaagg ttaagaacct gtcgcggggt
     1501 cgcgtgaggt ggggcacgtg ctgtgcgagt cgggctgagg cagcgtgccg accactccaa
     1561 ccgagccggc aggcgaggct tcacgccgcc ggcgcggaac agtgctcgat aagcaggtgc
     1621 acattattac ctgaaggtgt ggatgtcaac acctttccag acccgaccgc ttaccacttt
     1681 tgcggggcca tcggccaccc gccgcctcgt tttccgcgcc gtttccctgt acgttcggtc
     1741 acgacgagtg tcctgaaaac tactcgcgtc attcgcgttt taagtgccta ctgcttttcg
     1801 tcttccccgt ggccgccgcc gccgggtgtc tcgatgcgga cgcggtcgcc ggcctcaagc
     1861 tcgcgggagc ctttggcgtc gatcttgcgc gcgcgtcccc gtcggatgat ggagtcgcga
     1921 ccgggcttgc cgtccgcgcc gccatcgagt ccgtaggggc cgcggcggcg ccggtcggcg
     1981 agcagagaca tgcgcgccgg cacgagcgtt tcgatctcgc gcaccacgcc gtcgccaccg
     2041 ttgcgcgcgc cgcgcccgcc cgagcctttc cgcagcgcgt aggttctgac gcgcaaaggg
     2101 taggcgtatt cgagcgcctc ggccggcgtg ttgaggctgt tggtcatgtg cgtgtggaca
     2161 ccgccgacgc cgtcgcgcgc ggcgcgcgcg cccatgccgc ccgcgaccgt ctcgtagtag
     2221 gcgaactcct cgcccgtgcg cgcttctgtg ccgccgacgg tgaggttgtt catcgtgccc
     2281 tggctcgccg ccgggatgcg gccgggcagc gccttcgcga gcgccatgaa cagcacgtca
     2341 acgattcgct gcgaggtctc aacatttccg cccgcgaccg aagcgggcgg atttgcgttg
     2401 acgaccgtgc ccgccggcgc gacgacctca atcggctcca tcagccccgc gctcgccggc
     2461 acttcctcgg ctccgatgag acagcggaag acgtaggaga cggccgacac cgtgatggcc
     2521 tcgaccgcgt tgatcggccc ggcgacctgc ggcgcgctcc ccgtgaaatc cacgcgcgcc
     2581 cgctcgcccc tgatgcggac gctgacgcgc agagtcacct ccgtgcccgc gacgatgccg
     2641 tcgtcgtcga gcgcatctgc ggcctcgtat gtgccgtcgg gtatttcgcg gatgacggcg
     2701 cgcatgcgtc gggccgagta ggcgattaaa tggccggcgt actcggccga ctcgcgcgcg
     2761 cccgagcggc cgacaatttc cagcaggcgt gccgcacccg tccgcagaga ccccatctgc
     2821 gcctcgaagt ccgcgcgccg ctcgcgctcg acgcggacgt tggcgagtac gagccgcaac
     2881 acgtcatcga ccacgcgccc gccgcgcacg agatgcacgg gcgggatacg caagccctcg
     2941 ccgtacacgt ctgtcgctat ccccatcgag ccgggcgtcg ccccgccgat gtcggcgtga
     3001 tgggcgcggt tggcgacgta gaagagggga aggggtaaag gggaaggggt aagggtggga
     3061 gcgtcactga gacggttctg cttcgcgaaa cgtgccgttt ttctcccatt ctcctttacc
     3121 ccttcccctt taccccgctt cccatccccc ttcccctcgg cgtcgaagac gcccgcgacg
     3181 agtgtcacgt ccggcaggtg cgtgccgccg gcgaaggggt cgttgacgat gacaacgtcg
     3241 ccggggccga gcgtggtttc tttgagggcg gcggcgacgg ccagcggcat cgagccgaga
     3301 tggacgggca tgtggtcgcc ctgcgcgatg actctcccgc gcgcgtcgaa gacggcgcac
     3361 gagtagtcgc gccgctcctt gatgttgggc gagtgcgcgg tgcgccggag cgtgacgccc
     3421 atctcctcgg ccaccgaggt gtagagcgcg cggtagattt ccagcgtcgt cgggtcgaag
     3481 ccccgtccgt gtttcatggc tcgatgatta agttcccgcg gctgtccacg gacgcgcgcg
     3541 ctcccatcgg cacgagcgtc gtcgaagagt attcggtgat gaggcacggg tcacgcaggc
     3601 gcgctcccgc cggcagctcc gcgcgcgcgt agacggctac tcgcttgcgc cccgtctcga
     3661 aatgcacgag cgcgacggcg tgcggctcga tagttttcac ggacgagcgc cgccgcgaca
     3721 tgcgcgcagc ccgcggccct tcgacgaggc cgcgcgagcg cacgcgcgcg ctcacgatct
     3781 cgaccgcgcg cccaccctgc gcgtaaccgt aacgcgcctc gtgcgcgtga tggaagcgcg
     3841 cgatgagcgc cgcctcgccg ctgcgcttcc actctatctc caactcgaac gactgcccct
     3901 tgtagcgcgc cgtcagcgtg cgctcgtgtc gttgctcgtc gtcgccgaaa ccctcgcggc
     3961 gcagcacggc gcgggcctcg cgttccatct cagtgaacgc gcgttcgagc cgcgccgtcg
     4021 tcttcgcgtc gccggcatcg agcatcaccg tgcggctgag gtctttgacc acgtccgact
     4081 ggagcgcgcc gagggcggag agcgcgccgg ggtcggcggg gacgacgacg cgcgggatgc
     4141 gaagcgcgcg cgcgagttcg acggcgtgca ggccgcccgc gccgccgaac gtgacgagag
     4201 aataggcgcg agggtcgtgc ccgcgctcga ccgacaccac gcgcagcgcc cgctccatcc
     4261 cggcattgac gaccctcaac acgccgagcg ccgcacccgt ggccgtcacc cgacgcccgc
     4321 ccgccgacgc catctcccgc ccgagttttg tcaaagcctc ttcggcgcgt cgcgcgtcca
     4381 acgcgaattc gccgccgaga aggccgcctc cgccgaagcg tccgaggacg aggttcgcgt
     4441 ccgtcaccgt cggcaaaagc gaacggccgt agcaggccgg gccggggtca gcgcccgccg
     4501 actccggccc gacgcgaagg cttccgcccg cgtccacgcg cgcgacgctg ccgccgcccg
     4561 ccccgaccgt gtggatgtcg agcacgggca cggccaccgg aagcccggcc acgctcgcct
     4621 cgttcgtcag acgcagccgg ccgcccgcgc acagcgccac gtccgtcgag gtgccgccca
     4681 tgtcgaacgt gatgatgtcg gagaggcccg ccgcgcgcgc gcacgagagc gcgcccacta
     4741 cgccgcccgc cggccccgat aaaatcgtcc gcaccggctc gcgcgccgcc gcgacggccg
     4801 agatgctccc gcccgacgac tgcatcacgc gcaaagcggg gacgctcgac ccgacgctcc
     4861 gcaggtaagc gcccatcaac ggctgaaggt aagcgttgat ggcgaccgtc gaggtgcgtt
     4921 cgtactctct atactctgga aggattttgt gcgacaccga gagcgccacg tctttaagcc
     4981 catcggccag cgcgctctcg accttccgct cgtgcgccgg gttcgcgaac gagaaaagga
     5041 gcgagacggc gaccgactcg acgcgcgcgc gcttcaactt cctgacaagt gcggcgagcg
     5101 ccgcctcgtc caactcttcg accacggcgc cgtcgggcgc tatgcgaccg ccgacgccga
     5161 ggcgcaattc gtcggggacg agtggcgcgg gtcgctccca atcgagatcg tagagactac
     5221 cgcgcgcctg ccggccgatg acgagcacgt cctcgaagcc ggcgttggtg acgagcgccg
     5281 tgcgcgcgcc gcgccgctgc aagagcgcgt tcgtcccgac ggtcgtgccg tgtacaactt
     5341 gcgtatcacg cagggagact cccgcctcgc gggcgacgcg ccgcaggccg tcactgatgg
     5401 cgcgcgaagg gtcgtcgggc gtggaggcga gcttgaagat tttcgccgca ccattggcct
     5461 cgtagacgaa gtcggtgaac gtgccgcccg tgtccacgcc gaccctcacg cgccgcgtat
     5521 cttttctgcc tttcatttgg gaagagtttc cgacaggtcg tcccatccgt aagccagctc
     5581 gacgaaatcg acgccgccga tttgaagtgt cacgcggccc acggggcgac cgcccatgcg
     5641 ctcgtagaaa tttcggtagg gactcgcctc aagcgtttgc agatacatcg aacgccggcc
     5701 gcgcgcgagt aaggcgtcgg ccgtcaggcg aaagagacgg ccgccgacgc cgcggcgctg
     5761 ttgctccggc agcacgtaga tcgagtagag ttccgccgcg aacggcccga agcgcgtctc
     5821 ccgcggcggg ccgaagtcca cgtacccgac tgccgaagcg cctgcccgtt cggcgacgat
     5881 catctggtag aaatccgcgc cgccgtctgc aacgcgcgcg cgttcccttt gctcgaacct
     5941 ctcggcgaag gcggcggcgc gcttctcgta attcatacca tcgaggtgcg cctgcgggac
     6001 gacgccgcga aacgactcct gccacgagcg cacgttgacc acggcgacgg cgcgggagtc
     6061 ggcggacgtg gcctccctgt aagtgatctc ctcgtttgtt gagacaggca agatgttaga
     6121 cgcggcgtgc gccgtgaaat tggttttcag cgcgccggtt cagtcggtga cggtgacgcg
     6181 gcagccgctc tcgtgcgagt cgcgcgcggc gtcgagcacg cgctggatgt ggtagccgtc
     6241 gtcgaaagtg gccgcctcgc gcacgcgcgc ctgccggccc tcgccgcgca gggcttcgac
     6301 gatgcggcgc gagaactcgg tgaagccgcg cgaccactcg ttgtcgttca tgccagaagc
     6361 gagcggcgcc agatcgactt cgacgcgtcg ccactcgcgc ccggcggcgt cggcgcgaaa
     6421 gagcgcgccc gtcgtgtctg tacgcaaggc cccgcgtggg ccgaagacct cggtgacgtg
     6481 cattgactcg cccgattcga cgaccgaggc cgagacggcg gccgtggcgc cctcggcgac
     6541 cgcgccgccg ccgagccgga gcagcagcgt ggcctcgtcg tcggtcgtca cacgcttgag
     6601 ttctccggtc ttctcctcgg ggcgctcgga gacgtgtgtg gcgagcgtgc aggagacctg
     6661 tgtggtgcgc gcgtccgtca gccattggag cgaatcgacg gcgtgcgagc cgatggcccc
     6721 gaggacgccg ccgccctgct cggcgtcaga ccaccagtcc cacgctcgcc cggtgtcggc
     6781 gcgcgagccg gcgcggaaat gcacgcgtgc gtgccacacc tctccgacct ccccattcga
     6841 gatcatctcg cgcatcaact ggcgcgcggg caggaagcgc agctcgtggt cgatatgcgc
     6901 caagaggccg gcgcgctcgg ccgcgtcgcg catccgtgcg gcctcctcga agttcatcgc
     6961 cgtcggcttc tcgcacagga cggccttgcc ggcggacagg gcggcgagtg tcatctcggc
     7021 gtgggtcgcg ggcggggtga cgatgctcaa gaggtcaacg tcgtcgcgcg tcagaagctc
     7081 gcgccagtcg gcggtagcga aggggatgtc gaactcgctt gcgaccgctc cggcgctctc
     7141 gcggcgacgg ctcgcaatcg ccacgacgcg cgcgccctcg caagcgcgga aggcgggaat
     7201 ctgcgtagtg cgcgcgaacc cggcgccgat gatgccgata ccgatgcggt tgtctgtggt
     7261 cacccgttga aacctcatgt gtgatttggg gcgcagtata acgagagtcc gcgggcgttg
     7321 acaatttcgc cgccgccgcg ccccccgaca taaaaaagtg gcggagtctt ccatcagact
     7381 ccgccaccgt taatcacgca cgcggccgag ggccgagggc gtttgtctta gtgctgcttc
     7441 acgggccgac gtgcacctgc atcatgctgt tttgcgcgct cgtgttgacc accctgatga
     7501 tcgtgcccgt cttcgggacg atgacgccgg cggcaggcgt gtccgccgac cagtagctgt
     7561 tcaggtcgtt gaagaccggg acggccggca gcgacgggtg ggagaccgtg aactcgacga
     7621 cgcggttcgg cgtgcccgcg gggcttccgg ccgcaggcgg cagggttccc gtgaagggca
     7681 gcgtgagggc gtcggtcggc tccaggccga acgtcgaatc gaaggactgg atggtggctg
     7741 accagggcga gaacgtccag ttcagcccgt cataggggac gacgcggcgg agcggctccg
     7801 ggtgcgcgtc gatgggcagc gcgaggccct cgccccggtg ctggctggtg ttgtcgttgg
     7861 cctgatcctg atcccagtag gtgatgagca tcccgttctg gtacgggtag tgcgtgaccc
     7921 agctcgggcg ggtgagggcg tggccgaacg tgtaggggcc ggtctggagt ccctggtcgt
     7981 aggtgcggta ctgccggtac tcggcgatgt agtagtgcgc ggcgttcgag ctttcgatgc
     8041 cggtcgtggc cttgaagccg ccaagcgtcc agccgttgtc gccggcctca gcgccgtccg
     8101 agaaggcgcc gagcgtgatc tcgtcggcga ggaagccgtt gaggacggtg aaggcgtcgg
     8161 tcttgtagcg cagacgcagg agcacggtct ggccggcgta ggccgtcgtg ttgaaggagg
     8221 cgagtctcca cgcgccggcc gaggagccgg tgatgccgtt gccgaggttc tggccgttgg
     8281 cgttggtgtt ggtcgtcagc gccgactgga cggcgccgtt gaggtaggtg ccggcgaggt
     8341 tcgtccaggt cgcgccgttg ttggtcgaga ccgagacgta ggcatagtcc cagttggctt
     8401 cgatgttgta ctggagcctc atggtcagag ccgacgtgcc ggccggaacc tccacctggc
     8461 gaaccatgtt ggtgtcgagg ttgtcggcct tgccgcccca ccacgccttc gtgccgtact
     8521 ggagcttgcc ggcggtcggg tcgaagaggt agaagacgtt cttgtcggcg gggaggctga
     8581 cgacgacggc ctgcgccttc ttcgtgttcg tctccgcggg gccgagcttg tggtcggagg
     8641 ggacgcccgt cgtggctatg tcgtagttca gccagccgag ctggagctta tcccagccgc
     8701 cgaagtcgcc ggggcgcgag ccgatgtcaa tcgtcccgtc gccgaggtag gagccgctcg
     8761 acatgatcgt ccagaagccg gtggagttcg agccgccgtt ggtgtcgtag tggtcaggca
     8821 ggccgaggtc gtggccgtac tcgtgggcga agacgccgag gccgccgttt tcgggctgga
     8881 cggtgtagtc gccgacccag atgccggtct tggagagggg gtgcgcgttc tggacgttgg
     8941 ccgcgttgac gctggacgag ttgatgccct gaaccacggc tcctgaagag ctggtcgtgc
     9001 cgccgttcgg gttggcgccc cagccgccgc cgaactcgac gccgccgtcc ttattgtagg
     9061 aggggccggt gacgccggcg aagttgtacc acgcctgcca gcggtgcgac cagatggcgt
     9121 tggagccttg cgcgccgccg cccgtctcct cgcccgcgcc ggagtggacg atctggaagt
     9181 ggtcgatgta gccgtcaggc tcgtcgaagt tgccgttgtt gttgtagtcg tagcgatccc
     9241 acacgtcgaa ggactggagg tgggtcttga tctcatctag cgtcttgcct gaggcgagct
     9301 gatccttcgt ccagacgttg atggcgtcgg cgatcatcgt ccaggcgccg gcgtcgccca
     9361 tcaggttcga gccgtagcgc gggccgttga agggcacctt gacccactcg gtcacggcgc
     9421 cgttaatcgt gtagcggccg gaagactggg tctggtagta attgaccatc cggttgaagt
     9481 acatgtcata gaagtgggcg gggctgtagt cagcctgcca gatggtgttg ttgttgagag
     9541 cacgattcgg ctcggcgatc tggttgtgga gcggccccgg cgtcgtgggg acggccgcgt
     9601 tcgtgctggc ttgggtgccg tactcgacga ggacgacgaa cacgcggtcg ttccgctcaa
     9661 ggccgagttc gacgaactgg cccttggcga cctcgtgaac cttgccgttg ctcttcttgc
     9721 cggccagctt ctgcttgagg gcctcctgcc ggcgctggta gcggtcggcc gcgcgcggcg
     9781 acttgaagtt gtcgccgcca cggcgacgga atttgccctc gggcgagtcg tcgccgcccg
     9841 tgtcagacga ctcttcgccg ccgacttcct gcaattcgtt cagggaatct gcttcggaac
     9901 tgtcctcttg ctgcgccccg gcggggatgg aaatgaatgc tgcgatgagg gccacggaaa
     9961 gcagccacgt gatgaatctg taaggggtgt tgcgggtcgt tttagaactc actaatcgtt
    10021 acctccaggt gttcgttgaa agagatgggg ccggaagccg cgaggaggga gcctcgccgc
    10081 tcgggtaaca cctcgcttca gtgaggctta tcggggcgtc tttcgacaga cacacggagg
    10141 cccaagcgag gttggcgatt tgattctttt gttagctgat ttccccgaag gcttaagtgc
    10201 tgccgcagac agggaaccta cttacaagag ggcgaataac atacactggc aactcacacg
    10261 tcgtcaaacg tgcaatcaga tgcgccaccc ccgccgattt atgcacgcgc cgcgccggcc
    10321 cggagtaaaa atttacaagt tcttctccgc gccccgacgg ccggcacgct tctggagcgg
    10381 cccgcgcctc ggcgcgccgc aacgacccgc attgccgacc ttgacagccc ggccgcgcgt
    10441 ggctagcgaa ctgtgcaact gattggtgcc cccggtagga ttcgaaccta caaccaacag
    10501 attatgagtc tgctgctcta accgttgagc tacaggggca caaggattta cgggagattg
    10561 cttccaggct ggagggacat ttgtagggac tctgacccat ttattctcac agaccgagct
    10621 ttcttattca actcctccaa ccgttcaaaa gcccgctcca tgctttccgg cgtcggatgc
    10681 acatatcgct gagagacggt gacactgctg tgccccgcaa tctgcttgat ggtgaaggca
    10741 tccacgcctg cttcacccag cctggttaac atcgtgtgac gtaatgaatg tatcacaaag
    10801 tcttttgggg gttcgagcag acggcgcacc tttacgtgct ggtggtctag tgatgtgacc
    10861 tgaaacggct tatccttttt gcccggaaaa accagcgttg aattactctc taatgaacag
    10921 ctaagcaacc ccttaccgct tagtaaaaac aatttgtctg tagggcgtgc tgatcacgag
    10981 tcactgtttt tttatgctga tgcgctcagt ctcggtcacg gtcaccccag attgatatcg
    11041 gcgctgcctg acaacgcgaa gggcagagtt ctccaacgcc tccctgccgg gctgagggtt
    11101 agagacatat gcctccacta cccggccatt ctcaaccctc tttgtgacga cgacagcttc
    11161 gtcttccgaa gcgcgagact cctcattatt aagaggtttg gtaatagccg gcgggggaac
    11221 aggcggggga gaagcgggtg aggggtgtga ggtcgccgca agcacagtcg aattttcatc
    11281 ctgctcagat ggcggggctt tttgtttctg tctgggtcgt tcgcgggtga gtgcgccatt
    11341 ctcccctcgc tcgaccttcg gcgcgaaggt cgaatcggcc gcgtccgatg acgacttaag
    11401 aaggtcagag aatccatacg ttccaatcac ctcttccgtg tggctcgcca gcggcgcctc
    11461 cactttggcc ccgacggaat tcaacgacac ccccacaggt gaaacttccc tgacacgctg
    11521 attgaacagg cccatgtcgg atagaatcgt atacgacaga aaaattatca ccattatggc
    11581 aagcagccgg atgacaatcg acgagcttgt ttttaacgcc atcattaaac ccttcgacaa
    11641 gccacgcttc ccgtctttga ttcctcggcc caaccgacca ccggcgaccg tacctgatcg
    11701 atctccagag ccacccgaca gaggcaggca gggtatccga gtggttcgag gcgattacca
    11761 cgccttcact gcgccctcat tcaggaggcg tattccttaa gcggaggttg atatgtgtcc
    11821 tggaaaacct agacctagcg cctcatcctg tcagcgaaac cacccccgct ttgaaagccg
    11881 gggtgactaa aggcacgagc cgtttccgca agcacgcctg ccagcttagt gttgaggccc
    11941 ttaagaatct cgacttgatt cctcgccgag ctgatatctt tcgccccaag gtaagccatc
    12001 cccaagccga aatgataatc tgccgagccg gcacggagtt gtacagcccg cttatatgac
    12061 ttgacggcct cgggatagcg acggaggttg aagttgctca cgccgatgta atagtgggcg
    12121 tcggcggcgt ctggcttcag actgacgact ttcttgaacg cgttcagtgc gtcggcgtca
    12181 cgttccagtt cgaggtatga ggctcccagc ttaaagagca cttcatagtt gtcggggccg
    12241 aggcgggatg cctccttgaa ggccttaacg gcatctttat acttaccgaa tttatagtat
    12301 tcggagccga tttgagcata cttcttagcg tcggcctcct ccgaagactt cgccgccggc
    12361 ttagcctcgg aagtgtttac tgccgggcca atggttgagg attccgccgt ccgttcggag
    12421 tctgcgggcg ccgccaccaa gcttgcttcg gcaacggggg cggatgttac cgaagggggg
    12481 ccggcagaag gttcgaggtt gaactcataa ctcagcaggc cgaataccct cgccggttcg
    12541 ttcgacagtt gtgtcggcga gaagcgcgcg cggcgcgccg cctgctccgc cgcgtcggcc
    12601 agcaagacgt gcccagacac gacacgcgtc gaagagacct cgcccttctc gtccacaatg
    12661 acctgtacgt ctaccttgcc gacaaccttg gcggtagccg cgccaatggg gtataccggc
    12721 tggggcatgt aaacagcagc ccttctcaac tcttcgctgg acacgttctt tactaagaga
    12781 gtaggggccg ccgtgtcttc ctttgtgtct tcctttgtgt cttccttctt agcggccggc
    12841 tctgcttcat cggtcgtctt cgagagcgcg gcctgctcgc cgggtggagc agtggcgacc
    12901 ggcatgacgt tcggcggttt ggttttacct gccgccgggg cggtttcgtt ggccgccgcc
    12961 gcttgagggg tcgtcggatg gagcaggctc tcctccggtg tcggctcctt agccggggag
    13021 gcgccggcgt tacccgaact ttgtgtccca gagtttattg ccttggcgac cgcatcggtg
    13081 ctgtagtaag tgatggttcg gtaagtgact tcaatcggct cgtagctcag cttccccccc
    13141 tcgatgacgg cgaccgaggt ggtcatcttc gtccagttgc ccacggcatc gaactcgtaa
    13201 gcgtaggtct cccgactgat gaccgacccg tcgttggctt gcaaatccat ctcgatgaca
    13261 ttgcctttgt cgtcgaactt gcggtagacc tccttaccag ttaagttgtc ttcctcagtc
    13321 gtgaaatact cgttgtcggc cttcttacct ctacggtcat aaaggtctgt accttgcagc
    13381 ttgcgcggcc cctctactaa cccccccgac ttgacgatga tcttcgcaat ctccttacgg
    13441 acccggcgca ccggccccat caatccgtcc tcttgccgac taatgttgtc tgaagaaacg
    13501 acggtcgccg aggcgggggc ggcagtcttt tcctgcgcct cggcggaatg aaaggccgcc
    13561 gtataggaga gcgtcagaac cagtaggcgt atcggtccca gcatggagta cctccgagag
    13621 gctaaccgcg ggcataccct gggcggataa actgatgaac aacggcttca gactaaatgg
    13681 agggctggaa cgattaggct tatggtttta gcaggctcac actaccagcc ctgacaacga
    13741 aggtagaaat gtagcttact catccataac caacacgtca aatagttttt cttggatagg
    13801 gcgagcctgc tcccccaatg ttcagagtct ataagactca tccggcgggc tttgcccatt
    13861 aattcattga aagatgcaga aggagacaac gtcacccgaa tatgcagcgt taatgcactc
    13921 ctccctgaat taatgggcaa agctaaccag ggcacactcg gacgcatcct tacttgaagt
    13981 tatatgtgat tgtccctgac accttcgcag ccccgccggc cagcttctcg cttgagaatt
    14041 tcgcctcccg tgcggccttc acggctgcga ctcgcagtag cgggtgacca ttcagagggc
    14101 gcgctgaaat aacgtcacct tttttgttca ctacaaccgc gaccgtcacg ttgcctgaga
    14161 ccccttcgct tttaaccacc tccgggtatt ccgggctggg caatctaatc tctttgcctt
    14221 tgagcgcgcc gtcagttacc ggttgatccg gctgcacact ggcgggcgcc gtttgtgccg
    14281 agccgggcgc ggcgggaatc tgattggtaa aacttcggtt taactggtag ccgatgagac
    14341 cgcctcccaa gagtgcaaga gcgatcagga tcggcgccca cggccgcccg acgtttctat
    14401 tgtccctcat ggaaacgtat ggttctacag cggctgtgac tgaaggttca tcatcgctaa
    14461 ttaaacgtac ggagtcatac aagcagtacg ccagaatgtc gcttcgatag gtgctgttgc
    14521 acttcgggca gcgtctgacg ccggaacccg tgggcgtttc ttgagctgtg tgtccaagcg
    14581 tcgatgccgg gcgggtgggc tcgccataca tcacatcttc ataatggcgt cgcgctacct
    14641 cctctgattt ctgatgggct tctgcatcag cccgttcgcg aacctcttcg gcgatagcgc
    14701 cggcagcctt aaccgacgaa gttatctcgt cctcatcaga tgcgaccccg gccacgccct
    14761 gcccggcggg cgtggcctgc cgcatcgcgg cggggagcgg cgtctcgtcg gagtcgaggt
    14821 cgagttcgaa gtcggcggcc agggcggcag gctgcacgtg gctgaggtcg agcagcgcgt
    14881 cggcggcctg gggatcatgt gcccctctgg ctgtggcggt ttgtgcgtcc agaggctccg
    14941 tggattggcc gaggcgttgg gccgggttcg ccctctgctg tcgctcctcc ctgcctttta
    15001 tttctgccgg ttcttcgccc tgttgtcgta ccacttccgc agcttgtcta tagtcttcct
    15061 ccgccgcatt catcccgggg gtctttggac ccgcaggtgg ggtgtcttga agcagacgag
    15121 tcacttcatc gaagcctctc cttatcgacc actccgtcgc ggtgaggccc aagtaatcct
    15181 tcatccgcac atccgcgccc gcatggagca aaacacgcac cacgtcggca tggccgaaga
    15241 aggcggcgcc aatgagagct gtcacccctt tcagggtact cacattaacg tccgcgccat
    15301 cggccaggag ggcgctaacg gcgcccgcgt caccttgtgc tgccgcacgt atcagtggaa
    15361 tgtgagtaga agacagggtt caatcccttc tttgttaagt ccgaaattca gagggctact
    15421 gcacaacaca taattataag cagtaagaat gcctgaaatc catttagagc gaagggtcac
    15481 cttacctgct acggggatga aactgttgac gcaaacaatt ccacatcggg gcgcgtcaat
    15541 gcgcgtttcc cgaaagaagt gccgggcgat aatattggcg cgtgcggacg gccgcaagcg
    15601 tgcccaaaaa agcgttcgcg gccggcagtg acggccggca tttctgatat gctgcgacgc
    15661 cggctttgaa acttgtggag gacgcgatga tgagggtgct caggacggcg attcttcttt
    15721 ttagcataat gattttggcc gctggcccgg tcggggccgc ggcgacgacg ccgcagaagt
    15781 cggccgcgat gggaagggcg gagcgggcgc gcctcgccgg gcgcgtccgc tcagagtttc
    15841 tgcacgcgtg gcgcggctac aagaagtatg cgtggggcca cgacgacctg cgcccgctca
    15901 gcaaggcccc gcacgactgg tacgggcaga cgcttttgtt gacgcaggtc gatgcgctcg
    15961 acacgctcgt cgtcatgggg ctgcgcgccg aggccgacga gacgcgctcc tatatcgtcg
    16021 aacatctctc gctcgacaga gacgtctacg tcaagaattt cgagatcgtc atccggctcc
    16081 tcggcgggtt gctctcaggc tatcagatga gcggtgaccg gcggttgctc gcgctggccg
    16141 aagatttggg gatgcgtctg ctgccggcct tcgagtcgcc cacggggatt ccctatcgat
    16201 acgtcaacct caagacgggc aaggtgcgtg ggaacgtgac caacccggcc gaggcgggca
    16261 cgctgctcgt cgagttcggc acactcgccc gtctcaccgg ccgacaaatc ttctacgaca
    16321 aggccaagcg cgccctgttg gaaatctatc agcgccgctc gtcggtcggt ctcatcgggc
    16381 agcggatcga cgtggagacc ggccgctgga cgcacaccga cagccatctc agcgcggaga
    16441 tcgattcgta ctacgaatac cttctcaaat gctggctgct attcgacgac gccgactgcc
    16501 ggcggatgtg gcgggagagc gtggcggcgg ctaacaagta tctcgcggac gaagtgggga
    16561 cggaactctg gtacggccac gcggacatgt ggacgggccg gcgcgcggcg accacctacg
    16621 gcgctctcga cgccttcatc ccggccatgc tcgctctctc gggcgacacg gggcgcgcgc
    16681 gtcgccttca ggcgtcgtcg ttcaagatgt ggaacaagca cggcatcgag ccggaggaga
    16741 ttaattaccg gacgatggag gtgggtgatg ccggctaccc gctgcggccc gagatcgtcg
    16801 aatcggctta ttacctccat cacttcacgc gcgaccccgt gtatctgcgg atgggcgagt
    16861 cgatgttcga cgacttcgtc aaatactgta agactgacgt tgcctacgcg tctttgaaga
    16921 gcgtggtgac gaaggagaag gccgactcga tggagagctt cctcttcgcc gagacgttca
    16981 aatacttcta cctgctgttt gcgcccccga gcgcgcttcc gttcgagcag gtcatcttca
    17041 acaccgaggc ccaccccatc aggcggacgt ggcgagcagg cgctcgatct gccggcggtg
    17101 gcgatcttcg tggagcgcga cgaaggcgag ccactggtag agattgagcg ggccgaaggc
    17161 ggggtgcggg taagtgtgaa ccgacgcgcc gacggcctcg acgcgggggc gcaaggctgt
    17221 gagcgcggcg cgcgactccc gcaaggtggc gagcgagtcg gagacggatg cgccgctacg
    17281 tggaacggcc atctcgggag cctgatactt ctcgcgccgg gcgcgctcgg ccacttcgtc
    17341 gagcgcgacg agcgggccgg cgtccgcacc gggaggcgga gcgccgtcgg cctcggcctt
    17401 ggcgagcagc ctcccgacga gctggctcat cagcttctcg atgatggcga cgtgctcgac
    17461 gacctcggcg acgcaccagc gttcgggggc gtcgcgcaac gcctcatgtt cgacgccgag
    17521 accggcgagc gtcgtgtcga gccgcgcgcg gctttcgtcc agcgcctcga aaatctcggc
    17581 gacgttgtgg aaggtagtct tcattattcg ctccctttga tgacggcgga aaagtgtaag
    17641 gcgcgaggag tcattgggcg gcggccgtcg cgcgtgcggt gacgcgcaca cgcgcggcgc
    17701 gaatcaactg gcgacgtgcg ggcgtcgtgc gccggatggc ggccggcgcg gaagacggct
    17761 gaccagcgtc gcctgcgggt agagcttgac gacgtgccag cgttcatagc gcacggtcgg
    17821 cacaggctcg ttcgggaagt aacgcatgta ggccgtctgg acgaagagca gctccggctc
    17881 cgtccaggcg acgcgcacga ccgggttgaa agagttcagc accacgctcc cgacgggcgg
    17941 gactgcgccg ccggcggcac tatcgctact actactgccg ccgccctccg gcccctgctg
    18001 accggcgtcg gtgttcgcgg ccgcgccgcc cttcttgaac atcttcgcgt cgaactcgac
    18061 ggaggacgcc cacaacgctt cttgcagagg gaggttcgcg cccgtcggct cgccgcccgc
    18121 ggcgtcgtag acggccacgg tcttgtcgaa ggccgtggcg accttcgagg cgtcgggcga
    18181 ccagacgggc gcggcgtcgg cgaggcggtc gtcgagcaga cgctcctgag cgtcgagcgc
    18241 gatgaggcgc ggacggcccc ccggcgcttc gcccttgcct ttgcgcgcat tccactcggc
    18301 ctcggtgcag gcgagcgcgg cgaggagttg cgcgtcgggc gaccaggcca gctcgaagta
    18361 gatgaggccg tcgcgcgtgg tgagcgggcg gatgttgagg ccgtcgcgat cagacaggta
    18421 gacctgctcc gtgccgaagg ccgcgacggg cgcgatgatg ggggcgacgg tcggaacggc
    18481 gacgggcgga gcttcggccg cggggtcgcc gggcgaggcg tcgggcacgg ccacggacgg
    18541 cggcgcggtc gtctcgcggc cggggtcggg tgtggcggcc ggcgtggggt tgcgaaaacc
    18601 gaggaaggcg aagtgctgac cgtcggacga ccaggcgacc tcctcgtaga aggagccgac
    18661 catgttgggc ggcatgatgt tgcggaggaa gacgccctcg gacgagtaga ggtccatgcg
    18721 gaagtcgaga cccgtcgttt cgctcgtcgc gtagagggcg agcgcgcgct ggccgtcggg
    18781 tgacgtgacc gtgcgcagca gttcctcttc ggggcggcgc gtctcgaagt cgcgcgcgac
    18841 cgcttcgttc ttctccggcg caccgtcgcc cttcaactgc tctggcagat tctccgccgc
    18901 cacgtccgcc tcgaagcgaa aggcgagccg cacggccggc acgtcgcgca gcgtcttcgg
    18961 ccgcacgctc accgcgtcgc cggcttgctg acaagcggcc gccacgagcg acgccgagag
    19021 cgcgcacacg aagaggtaaa ttttcactga cgtgaatcgc accagattgc ctctcatctc
    19081 cgggccttga tgatagaaag cacgccgtga ggatgtcaaa gaaaccgaaa ggtcagtttt
    19141 tacgccgggg cagaaacacc ccgtcagagc cgacgagacc tgtgccgtct ccgaagctca
    19201 tcgacaggag gacgaactca acctttttgg gcgggggaat gttcctttcg gcgctatagc
    19261 cttcccaccc tttgacgaac ctctcctcca ccatgaaggt gctgctctca ccggggagaa
    19321 gcggcgtatc gctcggaagc gccttgaccg acaggtctat taactcgcct ctgccgtagt
    19381 gaaactggtg cgcgtaaatt gtcccgtcgt gcttgacctc gggcagacga agcaagaggc
    19441 caaagcggta aatcggcctc gtcccgatgt tctttacttc gacttcaagc tcgccgtacc
    19501 acttctcatt tcttaaatcc ttcacctttt cgagattctt cacggtcact ttcagcggca
    19561 cgtgtgaagg cagcgcacta acaaattccc tctcatcctt cccggcctcc tgagcattga
    19621 cgggcgccga gaacaccccg cacagagtga ggagaaggat taacggcccg gcgctttgca
    19681 accttctact catctgttcc tccatgcggg tttgcgcttc tcgatgaagg cgcggatgcc
    19741 ttcgtcggcg tcttcggtct tcatcagctc gttgaggtag aggtcttcga cgcgcgcgag
    19801 ggcgtcgtcg aaggttcggc cgcgcgccgt gtcgagcgcg cggcgcgtca tttcgagggc
    19861 gggggcggag agttcgcgca ggcgcgcgag cacctcctga agtttccctt cgagctgtcc
    19921 ctcttcgacg aggtggctga cgaggccgag gcggtgtgcc tcctgcgcgt cgatgagttc
    19981 gcccgtgagc agcaactcgc gcgcgcggcg ctcgccgatg actcgcggca gcaacacggc
    20041 ggcgacgggc gggaacaccc cgagcttgat ctcgggctgg ccgtagtggg cgcgcgtccc
    20101 ggagatgacg atgtcgcagg cggtcacgag ttcgcacccg ccgccgaggg ccgcgccctc
    20161 gactaaggcg acggtcggct tacccagttg ctccatcgtg cggaagatgg cgtggaagga
    20221 gtcgagcatc tggtagatgg tctcggcccg gtgctcctcg acggagacgc cggccgagaa
    20281 ggcgcgcgac cccttcgcgg cgtcgaagac gacggccacc acgtcgcgca gcgtcatgca
    20341 gtcggtgagc gcgcggtcta cctcgcgcat catcgcgatg tcgaagatgt tcaagggcgg
    20401 gcgcgcgaac gtcacgcgcg cgacgcggtc tgcgacgcgg aagcggatgc ttttgaagtt
    20461 gtcgctcatc gtcttcacgt gtggcgcgcg aggtcggcgc gcgcgcgagc cgcctccgtg
    20521 ttatcttcgc cgcgctcgat gcgattgcgc gcgcggcgtt cggatgatat cacggtgcgc
    20581 gcacgggcga acgtcgccgt accggcggga gtgtggtgtc gatgactgaa gacggaatca
    20641 gtattcgcga catcgtggag gttgaggaga tgcgcgccgt cgaggaactc cagatggagg
    20701 tctgggggat gcctgagcgc gacatcgtct ccgtcttcat gctgcgggcg acgaacgccg
    20761 cgggcggcgt cttagtcggc gccttcgacg gcgcaactct cgccggcttc gcctacggct
    20821 tcgtcgggct ggaagacggg cgcgcgatcc tccactccga catgctcgct gtgctgcccg
    20881 cctatcgcgg gcgcgatgtg ggataccgtc tgaagctcgc gcaacgcgag cgagcgctcg
    20941 cgcggggact gcgcgtgatg acgtggacgt tcgacccctt gcaggcccgc aacgcccgcc
    21001 tcaacttcgg caagctcggt gtcgtctccg cacgctactt cgtcaatttc tacggcgagc
    21061 gaagctccag cttcctccac tccaccggca ccgaccgcct ctgggtgacg tggctgctcg
    21121 acagcccacg cgttcgccag agggcggcaa cgacggcgcg cgtagcgttc gacgaatggc
    21181 cggcgcgcgc gcgcgaactg cccgcgctcg tgcgcgtcgg cgacaatgac gcgccggtgt
    21241 ttgaagaaga tgctgacgcg gcgtcgcaga tgagtgagcg cgtcctggtc gagattcccg
    21301 gcgacatcac cgcgctcgaa gagaggcagc ccgcgctcgc attggagtgg cggctggcga
    21361 cgcggcgcgc cttcacgtcg gccctcgccg ccggctatct cgtcgaagag tttttcagaa
    21421 tcgagcgcgc cgggcggcgc gtcggcgcat atctgctgac ggctggcagg acgccggaag
    21481 atttcgcgaa gtgagatgcc cgcctccggg gcgcgcgggg cgcgggtggc gtttgggaca
    21541 gccgcagcac gcggcccgga cgcgcgcccg ttaccttgcc gtcgcgccac acctcgacgc
    21601 cgcccacgaa gacgcgctcg atgccctcgc tgatgagctg cggctgttga aaggtggcgc
    21661 ggtcgcgcac gcgcgcgggg tcgaagagga cgaggtcggc gcgccgtccc gcgcgcacga
    21721 gtccgcggtc atggagtttg aggcgcgcgg ccggaagcga ggtcatcttg cgcaccgcct
    21781 ccggcagact gagccagccc tgctcgcgca cgtagcggcc gagcacgcgc gggtagctcc
    21841 ccgcgccgcg cgggtggcgg cccgcgatgc cgccgtcgct acagaccatc acccacggct
    21901 gctcgtagaa gacgcgcatg tcctcttcct tcatcgaccg gcagacgacg ctcgccccgc
    21961 cgtcgcggac gatctgcatg tactgctcga cggcgctcac gccgcgattg cgcgcgatct
    22021 cctccagagt cttgaactca tactcggggt gcgcgcggca gctcacaatc gtcacgttcg
    22081 cggccccgcc cacgtcggcg agcgcgcgcg ccacgtcgcc cgcgtcgtcg tgacggcccg
    22141 aagggacgag cacgcggatg gtcgagtgcc aggcgtcgta ggggtagcag tcggccgtca
    22201 cgtcctgccc gcgtcggcgc gcgcggttga tcagcgcgac ggcctcgcgg gcgcggcccc
    22261 agacgccggc cgtgccgagc ttgatgtggg agatttggac agggagtccg gcgtcgcggc
    22321 cgatgcggac ggcctcttcg agcgcctcga aggtgaggtc ggcttcgtcg cggatgtggc
    22381 tgatgtagat gccgccgtgg cgcgcggcca tgcgcgcgag cgcgacgacc tcttcggtcg
    22441 tggccccctt ccccgtctcg tactccagcc ccgtcgagag accgaaggcg ccctcgcgca
    22501 tcgcctcgtc ggcgagtgac gccatccgcc gcacctcttc gggcgtggcc gcgcgcgccg
    22561 tgtcctcgcc catcacgcgc gtgcgcaagg tcgcgtggcc gacgaacgtc agcacgttga
    22621 cggacgccgg ctcgcgctcc atcttcgaga ggtacgcgcc gacgggaaac tccgagccgc
    22681 cgtcctgtcc gatggcgacg gtggtaatcc cctgcgagat ttgcgtggcg gcggtcgggt
    22741 ctgtggagag gccgcccccg gagtggttgt gggtgtcgat gaagccgggg gcgatgacga
    22801 ggccgcgcgc gtcgaggcgg cgcgcccccg ccggcacgcg cacgcgacgg cgcgcgccgg
    22861 ccgattcgat gcggtcgccg cggacgacga cgacggagtc gcgcacgggc gcgcggcccg
    22921 agccgtcgat gagcgtcgcg ccgacgatgg cggtcaactc cggctctgcc tgattggacg
    22981 cggccgacga ctgctgcgac tgctgcgccg aaaccgtgcc ggcaatttcg agttgctgtc
    23041 tttgagagga gatgaataac gcggaggcga agaagaaagg caaaagtaaa aaggcaaaag
    23101 gcaaaagtga gaaggcaagc cgcttcttca cttttgcctt ttgccttttt acttttgcct
    23161 tcacttgcgt ctctacggtg aatttctctc tcatcgtcac tcaacccccc gacggttcgt
    23221 tgatgcccgc tcgttgaaac ctcagacgag cctccgcatt cgtccgtccg ttccgcttca
    23281 ttctctcccc gcctgagccg gcttcgggaa tggtgcgccg agccaggctt cgtaggtctt
    23341 catctgctca ggcgtgggcg cggagacggg gacgatgcgg taagcgccct cggtcttcga
    23401 gccgagcttg acgttgacag tgcgtatctc gtcgtgacgg aagacggtga gtgcaatcga
    23461 gtcgccgggc ttcttgtcag cgacgcgcgc gttaagcgaa gtgagcgacg cgcgatagcc
    23521 gtccacggcg acgatctggt cgttggcgta gaggccctgc tcgtaggcgg gcgtgcccgc
    23581 ctgcacaacg cgcacgacga ggcggtcgcc gtcctgtgct atctggccgc cgaggaaggg
    23641 cttcggggcg ggcgctctgg cgtcggccgc cggcgtcgcc gcgcccgtcg tgtcgagtct
    23701 gaggcccgcg gcttcgagcg cggcgttgta gttcaactcc tcgcgcccgc gcacgtaagc
    23761 acggaagaag tcttcgaggc tcgcgccggc ctgttgctcg gcgacgcgtt ggaagtcttc
    23821 tggggtgaag ttgcgattct tcttggcgaa ctctgtgtag agttggcgca gcacgtcgtc
    23881 gagcgagcgc gcgccggcgg tgcgcttgcg gatttcgagg tcgaggagga agccgacgag
    23941 gccgcccttg tcgtagtagg agatggagac gttgggcgag ttctcgtcgg cgcggtagta
    24001 cctgatccag gcgtcgaagc tcgcctcctc gacgctcatc ttctggcggc ccggcgtgtt
    24061 ctgcatggcc tcgatggcgc gggccagttg ttccagatag tccttgtcgg tcatcagccc
    24121 ggcgcggcgc acgagcaggg tgccgtaata gtcagtgatg ccttcggcca cccacagcag
    24181 gcgtgtgtag ttctcgccgg tgtagtcgaa ggggccgagc gcgtcggggc ggatgagctt
    24241 gacgttccag acgtggaagt actcgtgccc gacgagttcg aggaagccgc ggtaggtcgg
    24301 ttcggcgcgg aaggagaagg ggtcggcgat gagcgcggtc gagttgaggt gttcgaggcc
    24361 gccgccgccg cgcgggcgca gcatgagcag aaaggtgtag tcgcgatagg gcacgtcgcc
    24421 catcatcgcg actgtcgtct ccactacttt tttaacgtcg cgctccatcc gcgcggggtc
    24481 atagttgccc ccatcgccgt cgatgacgat gcggtgcggc acgccgcgca cgtcgaacga
    24541 caaagtttta aagttgctga cgagaaacgg cgagtcgtag agaatgtcga aattttcggc
    24601 gcggaaggta ttgcgctcgc cggaggcggg cagacccgtc gcgaccttcc agttggcgtg
    24661 cggcacgacg cgaagcgtgg acggggcgct caggtggcct tcggggtaca tcagcagcgc
    24721 ggcgttgttc cagaaggcgt ggcggtcgtg tagctcgttg gtgcgaacgg tgagttcgtt
    24781 cgagtagacg cggtagcgcg cgcgcagctc gcgtgcgccg gccggcgtgg tgacgcgcca
    24841 ggtgttcttg ttgaccttcg tccacgggag cgcgcggccc ccagagtctt cggccgcgaa
    24901 gtcctgcacg tggcgctcga actcgcgcac caagtaggag ccgggcgtcc agacgggcat
    24961 ccgcaggtca accgtggacg gcccctgctg cgccgtccag cgcaggcgca tctcgacttc
    25021 gagcaggtgc gtgtccggcc gcggcatcga gacggtgtaa ctgatctcgg gcgccgcctg
    25081 tcgttgcgtc gatgaggttg acgacgcgag agcaggcgtc tgccggcgcg tggcctgcgc
    25141 cgacacgtcg gacgacgacg acggcgcggc gagcagcagc agcagagacg cggcgagggc
    25201 gagggcgtgc gcagtgcgtg tgtggcgcat ccgtggaact tcctttgctc tttggattct
    25261 gcgggggaaa gctttcgctg gcgcgcatct tactacgaaa cggcgcgggc gcggaaacgc
    25321 ggcgcttgcc gccccggcgg gccgcgttgt aacttctcgg cgggcaacaa tccatcgacc
    25381 aacgttgggc aagccgggtc ggttccgcgc cggccgggag agaggagcta aatcatgtcc
    25441 gtcgtcgtat gcgccaactg tggaacgaag aatcgcgttg aggaggaagc ggcgcgcgag
    25501 cgccagcccg tgtgcggcag gtgcggggta caactcggag aggaggtggg gacagcaccg
    25561 gccgactttg ggaagcccga gaccgtgacg gacgcgacgt ttgcgggcgc ggtgctcggc
    25621 gcatcagagg ggccgcccgt cctcgtggac ttctgggcgg cgtggtgcgg accgtgtgtg
    25681 atgctcgcgc ccacgctcga ccagctcgcc gccgaatccg gcgggcgcta ccgcgtcgcc
    25741 aagctcaacg tggacgagaa ccccgcgacc gcctcgcagt tcggcgtccg cagcattccg
    25801 acgctcatca tcttcaaggg cggccgcgag gttgaacgcc tcgtcggcgc gcagccgcgg
    25861 caacagatcg ccgcgcgcct cgctgcacac gcttagattt ttgagttgag tgtgcggtag
    25921 gtcggattca ccgcagaggc gcagaggacg cagagttaac gcagagggtt gaatctctcc
    25981 gcgaaggcac acgaagaggc acgaacagga gttaactccc a
//
LOCUS       DASVES010000520         5417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_120625, whole genome shotgun sequence.
ACCESSION   DASVES010000520 DASVES010000000
VERSION     DASVES010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120625"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="carA"
                     /locus_tag="VF240_22215"
     CDS             complement(<1..888)
                     /gene="carA"
                     /locus_tag="VF240_22215"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7177991.1"
                     /translation="MKDSKPAILVLEDGRAFRGRSWGAAGEACGEMVFNTSMSGYQEV
                     LTDPSYAGQVVCMTYPLIGNYGVNKADAESARPWVEGFVVREASRVASNWRSEETLDA
                     YLKRWSLPAIDHVDTRALVRHIRERGAMRACLSAIDLDERSVLEKALRSAPMENRELA
                     SVVTTAEAYEVAAVSEEKFHVVCYDFGVKRNSLNELARLGCRVTVVPTSTPASEVVSM
                     RPDGVYLSNGPGDPASMTTVVSEIRRLVEAEVPTFGICFGHQLLGRAFGGTTYKLRFG
                     HRGGNQPVKDLVTGKVEITS"
     gene            complement(885..1307)
                     /locus_tag="VF240_22220"
     CDS             complement(885..1307)
                     /locus_tag="VF240_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7177992.1"
                     /translation="MLSRMFLAACCLAACADVARACGCEPLTTKESKKTSEVVFTGAV
                     IDSYRERGADGEEWRVRLSVEDFWKGDPGEEVIVYTSGECMVTFEPGKKYLVFAARQA
                     GRGRLITEVCRNTAPLDARTEELAKLGKPKQRRPKGTE"
     gene            complement(1340..2641)
                     /locus_tag="VF240_22225"
     CDS             complement(1340..2641)
                     /locus_tag="VF240_22225"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7177993.1"
                     /translation="MSDWLRPLLLMFYAPARAMAEARDRAPLGPAVLLAFAAQALLNL
                     YVMWPHLPEQFGALGRGAGVAAVAWLTASSLFFTAIVFVPVVILAGGMLERRAGLGLA
                     LRQEYAPAASTILYARAAASLLSLPFAALMRVGGGEAEYYVQARAWLETFAVQGGTTA
                     GELLAAKPFLLQEGFANAFLLPVFAMLSVAAVREVFRISWWRAAVAVVASGVAMLPLS
                     GLLGWMFDHILASPVLLLVLLFLMSSYLGEVMRGRRARASFRQNLEASTLNPADASAH
                     YNLGLLHLQRRELSEARARFERAVEIDPEEVDAHYQLGRIARAERRWADAIRHYGETV
                     ARNDAHSQHEVWREAGATYVSAAQFEDARDALGRFLERRQADPEGLYLMGRTQFGLGH
                     LREAKEWMQRCIEAVRTSPAYKYRAEKRWAAEAQQFLRSQA"
     gene            complement(2762..3493)
                     /gene="lepB"
                     /locus_tag="VF240_22230"
     CDS             complement(2762..3493)
                     /gene="lepB"
                     /locus_tag="VF240_22230"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7177994.1"
                     /translation="MFRRNNRQAYPFGDVGVGDDRDIEVRRLEQESRRHFWAEARLLM
                     RDLAVALMIATLVIVFIVQPVKVEGSSMLPRLHGGVAEEAGREGEEHSDRIFVNKLIY
                     YGMPRLERGDIVVFWFPEEPSKSYIKRVIGLPGEIVHACDNHVMIDGRALDEPYLDPQ
                     RNVNRYNLGRPSADSQLMCEQRVGCQGCVYVKPSHYFVMGDNRDNSSDSRSWGLVPEK
                     YIYGKALFRYWPPSEASVIRHEGEE"
     gene            3816..4169
                     /locus_tag="VF240_22235"
     CDS             3816..4169
                     /locus_tag="VF240_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7177995.1"
                     /translation="MIKLDIVNRVAERTGVPKMKAEQAVDALFHSMKEALARGERIEL
                     RGFGVFVVKPRKRGVGRNPRTGEEVAIPSGKTIRFKPGKELQAHGFDTEDHEDHDHDT
                     DSATGDGSGDTTPSF"
     gene            4261..5310
                     /locus_tag="VF240_22240"
     CDS             4261..5310
                     /locus_tag="VF240_22240"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7177996.1"
                     /translation="MSDSLRAVPAFVERERVSSERRRRHAARPTAREWVRHGGLFFLT
                     LLTATCAGTLFAVPQSRIAEPALPAATTPLDYLLFVPSYLLQLLSAVSAYAFAHPQYI
                     VEGATFACALLFILLAHEAGHYVACRLYGVDASLPYFIPAPPFFLAGTLGAFIKIKSP
                     IPSRRALFDIGVAGPLAGFVALIPVALAALVTAQPQFAPLPSEGLMILNDPPFLRLLA
                     PLLGVRDLAHIAPNPFYLAAWVGLLVTSLNLMPVGQLDGGHATYAFFGARMHARLGRL
                     AFVVVLLLAPLGWLWHGAPSGIVYAILLFVMLRVRHPQAEDEFEPLGRERVWAAALTL
                     LVFLLSFTPFPITIL"
BASE COUNT          897 a         1910 c         1697 g          913 t
ORIGIN      
        1 cgaggtgatc tcgaccttgc ccgtgacgag gtccttgacg ggctggttgc cgccgcggtg
       61 gccgaagcgc agcttgtagg tcgtgccgcc gaaggcgcgc cccaagagtt gatggccgaa
      121 gcagatgccg aaggtcggca cttccgcttc gacgagccgg cgaatctcgc tgacgaccgt
      181 cgtcatcgac gccgggtcgc ccggcccgtt cgacaggtac acgccgtcgg gccgcatgga
      241 gacgacttca cttgcgggcg tcgaagtcgg cacgaccgtc acgcggcatc ccagacgcgc
      301 cagctcgttc agggagttgc gcttgacgcc gaagtcgtaa cagacgacgt ggaatttttc
      361 ttcgctgacg gccgcgactt cgtaggcttc agccgtcgtc accacgctcg ctagttccct
      421 gttctccatc ggcgctgacc gcagcgcctt ctccagcacg ctccgctcgt cgagatcgat
      481 ggccgacaga cacgcgcgca tcgccccgcg ctcgcggatg tgccggacga gggcgcgcgt
      541 gtccacgtgg tcgatggccg gcaggctcca acgcttcaga tacgcgtcga gcgtctcctc
      601 cgagcgccag ttcgacgcca cgcggctcgc ctcgcgcacg acgaagccct cgacccacgg
      661 ccgcgccgac tccgcgtcag ccttgttcac cccatagttg ccgatgagcg ggtaggtcat
      721 acagacgacc tgcccggcgt aggaggggtc agtcagcacc tcctgatagc ccgacatcga
      781 cgtgttgaag accatctcgc cgcacgcctc gcccgcggcg ccccacgagc gcccgcggaa
      841 ggcgcggccg tcttccagaa cgagtatggc cggcttcgag tctttcactc ggttcccttc
      901 ggccgtcgct gcttcggctt gccgagcttc gccagctcct ctgtgcgagc gtcgagcggc
      961 gcggtgttcc ggcagacctc cgtgatgaga cggccccgcc ccgcctgccg cgccgcgaac
     1021 acgagatact tcttccccgg ctcgaacgtg accatgcact cgccggaggt gtagacgatg
     1081 acctcctcac cggggtcgcc cttccagaaa tcctcgaccg acagacggac gcgccactcc
     1141 tcgccgtcag cgccccgctc ccggtagctg tcgatgacgg cgccggtgaa gaccacttcc
     1201 gatgtcttct tcgactcctt cgtcgtcagc ggctcacacc cgcaggcgcg cgccacgtcc
     1261 gcacacgccg ccagacagca cgcggccaga aacattctgc tcagcatttt cttcatcatc
     1321 atcatctcgt tttgccccgt catgcctgcg agcgcaggaa ctgctgcgcc tccgcggccc
     1381 agcgtttctc ggcgcgatat ttataggccg gcgaggtacg caccgcctcg atgcaacgct
     1441 gcatccattc cttcgcctcg cgcagatgtc caaggccgaa ctgcgtgcgc cccatcaggt
     1501 acagcccctc ggggtcagcc tgtcggcgtt cgaggaaacg cccgagcgcg tcgcgtgcgt
     1561 cttcaaactg tgcggccgag acgtaggtgg cgccggcctc gcgccagacc tcgtgctggg
     1621 agtgagcgtc gttacgcgcg accgtctcgc cgtagtggcg gatggcgtcg gcccagcggc
     1681 gctcggcgcg ggcgatgcgg ccgagctggt agtgcgcgtc cacttcttcg gggtcgatct
     1741 cgacggcgcg ctcgaagcgg gcgcgggcct ccgagagttc gcgccgctgg aggtgtagca
     1801 ggccgaggtt gtagtgggcc gaggcatcgg ccgggttgag cgtggaggct tccagattct
     1861 gccggaacga cgcccgcgcc cggcgcccgc gcatcacttc gccgaggtag ctgctcatca
     1921 aaaacagcag cacgagcagc aggacgggcg aggccaaaat gtgatcgaac atccagccca
     1981 gaaggcccga gagcggcagc atcgccaccc cgctcgccac gacggccacg gccgcgcgcc
     2041 accaagagat gcggaacacc tcgcgcaccg ccgccaccga cagcatcgcg aagacgggca
     2101 gcaggaacgc gttggcgaag ccctcctgta aaagaaacgg cttcgccgcc aaaagctccc
     2161 cggcggtcgt cccgccctgc acggcgaacg tctccagcca cgcccgcgcc tgcacgtaat
     2221 actccgcctc gcccccgccc acacgcatca gcgccgcgaa cggcagcgac aacagactcg
     2281 ccgcggcgcg cgcgtaaaga atcgtggacg ccgccggcgc gtactcctgc cggagtgcga
     2341 ggccgaggcc ggcgcgacgc tccagcatcc cgccggcgag gatgacgacg ggcacgaaga
     2401 cgatggcggt gaaaaagagc gagctggccg tgagccaggc gacggccgcc acccctgcgc
     2461 cgcgcccgag cgcgccgaac tgctccggca ggtggggcca catcacgtac aggttgagca
     2521 gcgcctgcgc ggcgaaggcc aagaggacgg ccgggccgag aggggcgcgg tcgcgcgcct
     2581 ccgccatcgc gcgcgccggc gcgtagaaca tcaacagcag cggcctcagc cagtcagaca
     2641 tggtaagaaa gtaagtagtg agcagtgagc agtgagcagt aggcagttga agaagtagtt
     2701 cgattgcgtg gggtgaatat cgccattcga gttcgagccg cggacgctta ctgcttactg
     2761 ctcactcctc cccttcgtgc cggatgacgc tcgcctccga gggcggccag taacggaaga
     2821 gcgccttgcc gtagatgtac ttttcgggga cgaggcccca cgagcgcgag tcgctggagt
     2881 tgtcgcggtt gtcgcccatc acgaagtaat gagaaggctt gacgtagacg cagccctgac
     2941 aacccacacg ctgttcgcac atcaactgcg agtcggccga cggacggccc agattgtagc
     3001 ggttgacgtt gcgctgcggg tcgagataag gctcgtcgag cgcccggccg tcgatcatca
     3061 cgtggttgtc gcaggcgtgg acgatctcgc ccggcagccc gatgacgcgc ttgatgtaac
     3121 tcttcgacgg ctcctcgggg aaccagaaga cgacgatgtc gccgcgctcc agccgcggca
     3181 tcccgtaata gatgagcttg ttgacgaaga tgcggtcgct gtgctcctcg ccctcgcggc
     3241 cggcttcctc ggccacgccg ccgtggagac gcggcaacat cgaagagcct tcgaccttga
     3301 cgggctgcac gatgaagacg atcacgagcg tcgcgatcat cagcgcgacg gcgaggtcgc
     3361 gcatgagcag gcgcgcctcg gcccagaagt gccggcgcga ctcctgttcg agccgccgca
     3421 cctcgatgtc gcggtcgtcg cccacgccca cgtcgccgaa cgggtaagcc tgcctgttgt
     3481 ttcgcctgaa catatttaat gacgccccgt tggcttgcag cgcggcagac ccatccctgt
     3541 cgagcttgtc tttcggccgc tgcgctgccg gaagtcgagg ttagccgccg cgcgcgagct
     3601 ttgtcaacga ggcgaccgaa ggtcagaggt cagaggtcag aggtcaatta aaaaccgctc
     3661 ttcactaacc tctaacctct gacctctaac ctctgttcta atccttgaca gcccctccgg
     3721 cgcgtctcta ttatgcgacg taaagtgttg ccactcttaa agaaatcagc gccggggcgg
     3781 cacgcgcggc aggcgctgac ggcaggaggg aaagcgtgat caaactcgac atcgtgaacc
     3841 gcgtggccga gcggacgggc gttccgaaga tgaaagccga gcaggcggtg gacgcgctct
     3901 tccactcgat gaaggaggcg ctggcgcgcg gcgagcgcat cgagctgcgg ggctttggcg
     3961 tcttcgtcgt caagccgcgc aagcgcggcg tgggccgcaa cccgcgcacc ggcgaggagg
     4021 tggccatccc ctccggcaag accatccgct tcaagccggg caaggaactt caggcccacg
     4081 gcttcgacac ggaagaccac gaagaccacg accacgacac agactccgcc accggcgacg
     4141 gcagcggcga cacgacacca agcttctgaa ataagaggtc agaggttaga ggttagaggt
     4201 cagtgaaaga caagatgctc tttactgacc tctaacctct aacctctgac ctctgctcat
     4261 atgtctgatt ctctccgggc agtaccagct tttgtcgagc gtgagagggt gtcgtctgag
     4321 cggcgtcgtc gccacgcggc gcgcccgacg gcgcgggagt gggtgcgcca cggcggcctc
     4381 ttcttcctga cgctgctgac ggcgacctgc gccgggacgc tcttcgccgt cccgcagagc
     4441 cgcatcgccg agccggcgct gccggccgcc accacgccgc tcgactacct gctcttcgtc
     4501 ccgtcctacc tgctgcaact cctgagcgcc gtgtcggcct acgcgttcgc gcatccgcag
     4561 tatatcgtcg agggcgcgac ctttgcgtgc gcgctcctct tcatcctgct cgcgcatgag
     4621 gccggacact acgtcgcctg ccggctctac ggcgtggacg cctcgctccc ctacttcatc
     4681 ccggcgccgc ccttcttcct cgccggcacc ctcggcgcgt tcatcaagat caagtcgccg
     4741 ataccttcgc gccgcgcgct cttcgacatc ggcgtggccg ggccgctcgc gggtttcgtg
     4801 gcgcttatcc ccgtggccct cgcggcgctc gtcaccgccc agccgcagtt cgcgccgttg
     4861 ccctccgaag ggctgatgat cttgaacgac ccgcccttcc tgcgtctgct tgcgcccctg
     4921 ctcggcgtgc gcgacctcgc gcacatcgcg cccaatccct tctacctcgc ggcgtgggtg
     4981 ggcctgctgg tgacgagcct caacctgatg ccggtcggac agctcgacgg cggccacgcc
     5041 acctacgcct tcttcggcgc gcgcatgcac gcgcgtctgg ggcggctggc gttcgtcgtc
     5101 gtcctcctgc tcgcgccgct cggctggctc tggcatgggg cgccgagcgg catcgtctac
     5161 gcgatcctgc tcttcgtcat gctgcgcgtg cgccacccgc aggccgagga cgagttcgag
     5221 ccgctcgggc gcgagcgcgt ctgggccgcg gcgctcacgc tgctcgtctt cctgctgtct
     5281 ttcacgccgt tccccataac catcctctag cgcgcctacc aacctttcct cgggtcgcta
     5341 aaatgaaacg ctgtagctca cgcccggatt gcgcccgcgc tcgaacacaa cccccacttg
     5401 atgccgccca cgcgtgt
//
LOCUS       DASVES010000521         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1147404, whole genome shotgun sequence.
ACCESSION   DASVES010000521 DASVES010000000
VERSION     DASVES010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147404"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1538)
                     /locus_tag="VF240_22245"
     CDS             complement(81..1538)
                     /locus_tag="VF240_22245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7177997.1"
                     /translation="MSNELDACPGCGAKVAGAAIEVPDATEETSEASAVETQAAALAT
                     ASEAAPVSTPAATPQRGQGASASTKKAIIAAAVAIVACLGIIFWQAKVGSAHAVQISA
                     EDMSLIAEGLAPRQRARLSSDAAYRKQVAGDLRQILALAEEARRAGVADRPAVRSQLE
                     LGRSFLIAQNYFKKQSEAKLQPEQIVSKEEIDAYLKEPGAEKRFEDALAVVQAQQPTP
                     SKELTEEEKSDFRDQWARIMLGERKGLAAGLDRERKVQLQIQLQEATTLARTLAEDNA
                     KKFTATEEDAKAYVDGARAKAEEVLKRARGGEDFEKLAKEFSTEPGAEQRGGDLGWFG
                     RAQMVKEFSDAAFALKEGEISDVVQTEYGYHIIKSEGRRTAPGEQGGEAEEQVKARHI
                     LFLTKSEEVKQVLEERKRKDYFDSLTKQTKVSVAEDFTVAAPPPTPGAGFPIPGAEGP
                     QGPPPAEVELPAEEPASAKPAPAGKTGSQPKRRNR"
     gene            complement(1664..2977)
                     /locus_tag="VF240_22250"
     CDS             complement(1664..2977)
                     /locus_tag="VF240_22250"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7177998.1"
                     /translation="MSKKLFLATLAALLLLPLASHAQKKAPRSRTQPAGVGLTAQDMT
                     LIVEGLGFPPNVVAQLAGSADERRAFAKDIREMLAVAEEAKAAGYAARPELALQLELS
                     RAFVIAQAYFKSREGAGASAPEQVVSPAEIEAFYKGPGVAPQFEAFVADYQKNAPTKN
                     APITDAQRAELRAHYGRVMVGRQKGIAAGLDRQRKTQLTVMLQQSRLLAGAYSRDNLS
                     RYKATEQEIDAYIATHPELDTKQQRAKAEDVLKRARAGEDFAALAGQFSTEPGAKERG
                     GDLGWFGRGQMVKPFEDAAFALAKSGDVSDVVETQFGLHVIKLEGRRTQDEAGKPVEQ
                     IRARHILIGYDPARGGGRPVSPRDKARESVETEKRDKWLGDVVARRRVMVAEDYAVGP
                     GASKSVRDIDGAPTTTPTQPAATTGKRTAAGKRTPTRRRAPARRP"
     gene            complement(2986..3426)
                     /gene="mce"
                     /locus_tag="VF240_22255"
     CDS             complement(2986..3426)
                     /gene="mce"
                     /locus_tag="VF240_22255"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX7177999.1"
                     /translation="MKIEHIGIATPRLDDALRFWRDALGLEVTHTEVVEEQGVRVAML
                     PAGEPRIELLEPTGPASPVAKFLEKRGPGIHHIAVRVPDIRAALARLKESGARLIDER
                     PRVGAEGCLVAFVHPTSAGGVLLELVEHHGGDDDETHTAETERQ"
     gene            complement(3581..>4522)
                     /gene="meaB"
                     /locus_tag="VF240_22260"
     CDS             complement(3581..>4522)
                     /gene="meaB"
                     /locus_tag="VF240_22260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX7178000.1"
                     /translation="RNFFNGETRALARVITHVENRSPDSFEILRELFPHTGRSQVIGI
                     TGSPGAGKSSLVDRLALAYRQRGKTLGIIAVDPSSPFTGGAILGDRIRMQTFGIDPGV
                     YIRSMASRGHLGGLAGATADVAGVLDAAGKDPIIVETVGVGQDEIEIVRLADISIVIL
                     VPGMGDDIQAIKAGIMEIGDVFVINKADREGVLRTEKELEALLSLAERPDRWEPPIVK
                     TVATEGRGIEDLAAAVGRYHEFQKTADEGRARRRSIARWRLLELLRERLLARALDADG
                     AGAQLDRLALEVADKRRDPYSAVEEFIAAGTLGEPRS"
BASE COUNT          652 a         1554 c         1436 g          880 t
ORIGIN      
        1 agaataaaag caagagcgcc ctcttcactc agcgccttct tcattctgac tcctgactcc
       61 tgactcctga ctccctcttc ctacctgttc cgtctcttcg gctgcgagcc ggtcttgccg
      121 gcgggggccg gcttggcgct cgccggttct tcggccggca gttcgacttc ggccggtggc
      181 ggaccctgtg ggccttcggc tccggggatg gggaagccgg cgcccggtgt cggcggcggc
      241 gcggcgacgg tgaagtcttc ggcgacgctg actttcgtct gcttggtgag cgagtcgaaa
      301 tagtccttgc gcttccgctc ttcaagtacc tgcttgacct cctccgactt ggttaagaac
      361 aggatgtggc gcgccttcac ctgctcttcg gcctcgccgc cctgctcgcc gggggccgtg
      421 cgccgcccct cagacttgat gatgtgataa ccgtactcgg tctggacgac atcgctgatt
      481 tcgccctcct tgagcgcgaa ggccgcgtcg gaaaactcct tgaccatctg ggcgcggccg
      541 aaccagccaa ggtcgccgcc gcgttgctcg gcgcccggct cggtcgaaaa ctctttggcg
      601 agtttctcga agtcctcgcc gccgcgcgcg cgcttgagaa cttcttcagc cttcgcgcgt
      661 gccccatcga cgtaagcctt cgcgtcctct tccgtcgcgg tgaacttttt ggcgttgtct
      721 tcggcgagcg tccgggccag cgtcgtcgcc tcctgaagct gaatctggag ctgcaccttg
      781 cgctcgcggt cgaggcccgc ggccagcccc ttgcgctcgc cgagcatgat gcgcgcccac
      841 tgatcgcgga agtcgctctt ctcctcctcg gtaagttcct tggacggcgt cggctgctgc
      901 gcctgaacga ccgccagcgc gtcctcgaaa cgcttttcgg cgcccggctc cttcaggtaa
      961 gcgtcgatct cctccttcga cacgatctgc tcgggctgga gcttcgcctc actctgcttc
     1021 ttgaagtagt tctgcgcgat gaggaaggag cggccgagtt cgagctgcga gcgcacggcg
     1081 gggcggtcgg ccacgccggc gcggcgcgcc tcctcggcga gcgcgagaat ctggcgcagg
     1141 tcgcccgcca cctgtttgcg ataggcggcg tcgctcgaca ggcgcgcgcg ctgccgcggc
     1201 gccagcccct cggcgatgag cgacatgtct tccgccgaaa tctggacggc gtgcgcgctc
     1261 ccgaccttcg cctgccagaa gatgatgccg agacacgcga cgatggcgac ggccgccgcg
     1321 ataatcgcct tcttcgtcga ggcagaggcg ccctgcccac gttgcggcgt cgcggccggt
     1381 gtcgaaaccg gagccgcttc cgatgcggtc gctagtgctg ccgcctgtgt ctctaccgcc
     1441 gacgcctccg atgtctcctc agtcgcgtcg ggcacttcga tggccgcccc ggccactttc
     1501 gcgccgcaac cggggcaggc gtccaactcg ttagacattt gtgttccgca ttctctgcaa
     1561 acgatcatga ttcttccttt ttattgaatg attttcgggc cgcttccgcc acccgttcgg
     1621 ttgtgcgccg acagattaaa tgaaatgcgc gacggtctcc aattcacgga cgacgcgcgg
     1681 gggcgcgcct ccttgtcggg gttcgcttgc cggcggccgt tcgcttgccc gtcgtcgcgg
     1741 ccggctgtgt gggcgtggtc gtgggcgcgc cgtctatgtc tcttacggac ttagacgccc
     1801 ccggccccac cgcgtagtct tccgccacca tgacgcggcg gcgcgccacg acatcgccca
     1861 gccacttgtc acgcttctca gtttcgacgg actcgcgggc cttatcgcgc ggcgaaacgg
     1921 gccggccgcc cccacgcgca gggtcgtagc cgatcaggat gtgacgcgcg cgtatttgct
     1981 cgacgggctt gcccgcctca tcctgcgtgc gccgtccttc caacttgatg acgtggaggc
     2041 cgaactgtgt ctcgaccaca tcgctcacgt cgcccgactt ggcgagcgcg aaggccgcgt
     2101 cctcgaaggg cttgaccatc tgcccgcgac cgaaccagcc gaggtcgccg ccgcgctcct
     2161 tcgcgcccgg ctcggtcgag aactgtccgg cgagcgcggc gaagtcttca ccggcgcgcg
     2221 cgcgcttgag aacatcttct gccttcgcgc gctgctgttt tgtgtccagc tccgggtgag
     2281 ttgcgatgta ggcgtcgatc tcctgctcgg tggctttgta gcgcgaaagg ttgtcgcgcg
     2341 agtaagcgcc ggccagcagg cgtgattgct gcaacatcac ggtgagctgc gtcttgcgct
     2401 ggcggtcgag gccggcggcg atacccttct gccggccgac catcacgcgc ccgtagtgcg
     2461 ctctcaactc cgcgcgctgc gcgtcggtga tgggcgcatt cttagtgggg gcgttcttct
     2521 gatagtccgc gacgaacgcc tcgaactgcg gcgccacgcc cggccccttg tagaacgcct
     2581 cgatctcggc cggcgagacg acctgctccg gcgcgctcgc ccccgcgccc tcgcgcgatt
     2641 tgaagtaagc ctgcgcgatg acgaaggcgc gcgaaagctc caactgcaaa gcgagttcgg
     2701 ggcgcgcggc gtagccggcg gccttcgcct cctcggccac cgcgagcatc tcgcgaatat
     2761 ctttggcgaa cgccctgcgc tcgtccgcgc tcccggcgag ctgcgcgacg acgttcggcg
     2821 ggaacccgag accttcgacg atgagcgtca tgtcctgcgc ggtgaggcca acgcccgccg
     2881 gctgcgtgcg gctcctcgga gccttcttct gcgcgtgaga cgcgagcggc agcaggagaa
     2941 gggcggcgag ggttgcgagg aaaagttttt ttgacaagtg caagactact gcctttctgt
     3001 ttcggcggtg tgagtttcgt cgtcgtcgcc gccatggtgt tcgacgagtt cgagcagcac
     3061 gcccccggcc gaagtgggat ggacgaaggc gacgagacac ccttcggcgc cgacgcgcgg
     3121 gcgttcgtcg atcagacgcg cgccgctctc cttgagccgt gcgagggcgg cgcggatgtc
     3181 gggcacgcgc acggcgatgt gatgaatgcc ggggccgcgt ttttcgagaa atttcgcgac
     3241 tggcgaggcg gggccggtcg gctccagaag ctcgatacgc ggctcgcccg cgggcaacat
     3301 cgccacccgc acgccctgct cctcgaccac ctccgtgtgc gtcacctcca gccccagcgc
     3361 gtcgcgccag aagcggagcg cgtcgtcgag gcgcggcgtg gcgattccga tgtgctcaat
     3421 cttcatcaat caattctcga actacgtcag atgccgaact ctccgcccct gctgcccatt
     3481 ccgcccgttt ttctttgcgc ctccgcgcct ccgcgccctc tgcgttaatc cttagagcgc
     3541 gaccaaaagt ttacgcagag ggcgcagagg cgcagaggaa tcagctacga ggctcgccca
     3601 aagttccggc ggcaatgaac tcttcgacgg ccgagtaggg atcgcggcgc ttgtcggcga
     3661 cttcgagggc gaggcggtcg agttgtgcgc cggccccgtc ggcgtcgagg gcgcgcgcca
     3721 gcaggcgctc gcgcaggagt tcgagcagac gccagcgggc gatggagcgg cggcgcgcgc
     3781 ggccctcgtc ggccgtcttc tgaaactcgt ggtagcgccc gacggccgcc gccaaatctt
     3841 ctatcccgcg cccttcggtc gccaccgtct tcacaatcgg cggctcccag cggtcgggcc
     3901 gctcggcgag cgagagcagc gcctccagct ccttctcggt gcgcagcacg ccctcgcggt
     3961 cggccttgtt gatgacgaac acgtcgccga tctccatgat gccggccttg atggcctgaa
     4021 tgtcgtcgcc catgccgggc acgaggataa caatggatat atcggcaagc cgaacgattt
     4081 cgatttcgtc ctgaccgacg ccgactgtct ccacgataat cggatcctta cctgccgcat
     4141 ccagaacgcc ggcgacgtcc gcggtggcgc cggccagacc acccagatgg ccgcggcttg
     4201 ccatgctgcg tatatacaca cccgggtcga tgccgaaggt ttgcatgcgg atgcgatcgc
     4261 ccaggattgc gcctccggta aacggactgc ttggatctac cgcaataatg cccagggtct
     4321 tgccccgttg cctgtaagcc agcgccagcc ggtccaccag cgagctcttg ccggctcccg
     4381 gactccccgt gatgccgatc acctgcgatc ttcccgtgtg agggaatagc tcacgaagga
     4441 tttcgaagct gtccggcgag cggttttcga cgtgtgtgat cacgcgcgcc agagcccgtg
     4501 tctccccatt gaagaaatta cg
//
LOCUS       DASVES010000522         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_897802, whole genome shotgun sequence.
ACCESSION   DASVES010000522 DASVES010000000
VERSION     DASVES010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897802"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VF240_22265"
     CDS             <1..345
                     /locus_tag="VF240_22265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178001.1"
                     /translation="DLRIEAASAQALPDDRYRVTATVLGRKTFDPAGGVKTTEVPLDE
                     MIDVAVYASHPLSTNAAPLYAGKHRLRTGQTEVTFEVPGPAGFISLDPFERRIEAERA
                     DNIRELQVSPRP"
     gene            complement(482..555)
                     /locus_tag="VF240_22270"
     tRNA            complement(482..555)
                     /locus_tag="VF240_22270"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(519..521),aa:Pro,seq:ggg)
     gene            complement(642..1196)
                     /locus_tag="VF240_22275"
     CDS             complement(642..1196)
                     /locus_tag="VF240_22275"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7178002.1"
                     /translation="MMNSAAVSIPEKLFFKIGEVCEITGIQAHVLRYWESEFPMLAPQ
                     KNRAGQRTYRKRDVEMVLRIKELLYEDQYTIAGAKKKLSNDLRGASKLKVVTPEMGAQ
                     LAAQQQQSYAPGAEAPDEMSDETPAPPARTLSSPAHNYAAAAPEQYDDTDERRAAVRR
                     LKEQMRELLVLLESSDPRRSAHRK"
     gene            complement(1438..2439)
                     /locus_tag="VF240_22280"
     CDS             complement(1438..2439)
                     /locus_tag="VF240_22280"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HEX7178003.1"
                     /translation="MKNRVLAIPVVLSLLLTIQWAAPLASGAAHSSVYDRFLSTLHFA
                     SNAPEAFQQRDGAGIGLPAPARLHEADGRGLLVQTWVNGTGPFEFAVDTGAGANILSR
                     RVASAARVEVETGGGAVRVVGLSGVGAQGARKAHVRNFALGARGNLLPSQGFTIIADG
                     LPAGVDGVLDPTESFSPLGYRIDMPNETISAFDPRLTPLRSDAAPADGTVARWLTEPE
                     SRRPYVMLAQGRRALIDTGSGFGLAVNRDAARSLGIFTGEGRERVGTRDLGGGDIPSR
                     RIRPATVHVGSLALRNVPTDYLSDAGAGAPVLLGRDALRPFELSFDPVNRLIRFKPV"
     gene            complement(2476..3075)
                     /locus_tag="VF240_22285"
     CDS             complement(2476..3075)
                     /locus_tag="VF240_22285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178004.1"
                     /translation="MILRNKLVPVALGAALLGGSVGAFVMRPTKSAETQVASAVSTTP
                     STATAPAKNFIPASTTADTQALPANNTENECYREGFAEGFRAAREEGLESASAVAAPT
                     RAGTTERVAYRSAPRTRYVRSRSAGNSRQVYYDYNQPQKRSFWQKHRDKLTVAMGAGG
                     GALIGGLIGGKKGAGIGALAGGGGSALYTYKLRKRDRRY"
     gene            3461..4624
                     /locus_tag="VF240_22290"
     CDS             3461..4624
                     /locus_tag="VF240_22290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7178005.1"
                     /translation="MSTSVFRLASRLDAVGFSDIVKIRNRVMQLRASGAQVFQFEGGE
                     PFLNTPDPVKEAMMQALRENRTRYAPSSGIPELRAAIAAKLRERNCIEATEEDVVVLN
                     GGMQGLFGAFQSVVNPGDEVLVFSPFWTPIKDLIAHCRGVVVLVPTEEARREGFRETL
                     ARYSTERTRAVYYNTPQNPTGVVYTRDEAETVARFARERDLVVLADEAYEDLVYGGEH
                     VSIASLDGMAERTITSFTLSKSYAMTGWRVGYAVAKEPWMAGLKKCLLYSTNGVSTPS
                     QWAALAAFALPEDFYVTMRRAYRERRDLLISGLCEIGLACAAPAGAFYAFPDVSSINP
                     DSRVAAETLLERAQVATVPGIVFGEHGEGHLRFSFSLPKETIEAGLDSMRRNL"
BASE COUNT          826 a         1542 c         1600 g          841 t
ORIGIN      
        1 gacttgagaa ttgaggccgc ctccgcgcag gcactgcctg acgaccgcta ccgcgtgacc
       61 gcgacggtgc tgggccgcaa gacgttcgat ccggccgggg gcgtcaagac gaccgaggtc
      121 ccactggacg agatgatcga cgtggccgta tacgcgagcc acccgctcag cacgaacgcc
      181 gcgccgctgt acgccggcaa gcatcggctc cgtaccggcc agaccgaggt gaccttcgag
      241 gtgcccggcc cggccggctt catcagcctc gaccccttcg agcgacgcat cgaggccgag
      301 cgcgccgaca acatccgcga gcttcaggtg tctccgcggc cctaacgccc ggcacgggga
      361 agcatcacgg gggagccggc cacgtgccga ggagccgaca gatcgcgtcg tgcatctgct
      421 cgaggcgaat gtgggttccg gctgctcagg cgagcaagcc cttagttgtt gattgggtta
      481 atcgggatga ctggattcga accagcgacc tctcgcaccc caagcgagcg cgctaccagg
      541 ctgcgccaca tcccgtcgta aggcggtgag tagtatggcc ggaagaagtc gctccggtca
      601 aaccgcgaag gaagtggaaa cggtggagaa ggcgagacgc ctcacttgcg gtgtgcgctg
      661 cggcgggggt cgctcgattc gaggagcacg aggagttcgc gcatctgctc cttgagacgg
      721 cggacggcgg cgcgtcgctc gtccgtgtcg tcgtactgct cgggtgctgc ggcggcgtag
      781 ttgtgagcag gtgaagacag tgtgcgggct ggcggggcgg gcgtctcgtc cgacatctcg
      841 tcgggagcct cggcgccggg cgcgtaagac tgttgctgct gtgcggcgag ctgcgcgccc
      901 atctcgggag tgacgacctt gagcttgctc gcgccgcgca ggtcgttcga gagtttcttc
      961 ttggcgccgg cgatggtgta ctgatcttcg tagagcaact ccttgatgcg aagcaccatc
     1021 tccacgtcgc gcttgcggta ggtgcgctgg ccggcgcggt tcttctgcgg cgcgagcatc
     1081 ggaaactccg actcccagta gcgcaggacg tgcgcctgga tgcccgtgat ctcgcacacc
     1141 tctccgatct tgaagaagag cttttcgggg atggagactg ccgccgagtt catcattcaa
     1201 agcctttcgt cgaggccata ctgctaatcg aaggctgtaa gtttggcaaa ctgcccgcgc
     1261 gaccgacgcg ccgcgtgaac gcgtgggaaa acctgcggcc atgttactcg cgcccctaag
     1321 cgttgtcaac attattgtta tgccacgctt acgcccattc gcacgacggc gccggctgct
     1381 acggatgttg ccacacggaa gtcttgttgg ggcgagagag ttttttgcgg cgcgcgctca
     1441 gacgggtttg aagcggataa ggcggttaac ggggtcgaag gaaagctcga aggggcgcaa
     1501 cgcgtcgcgg ccgagaagga cgggggcgcc ggctcccgca tccgacaggt agtctgtggg
     1561 gacgttgcgc aacgcgagcg agccgacgtg gacggtggcc ggtctgatcc ttcgcgacgg
     1621 gatgtctccg cctccgaggt ctcttgtccc caccctctct cttccctcgc ccgtgaagat
     1681 gccgagcgag cgggccgcgt cccggttgac cgccagcccg aagccggagc cggtgtcgat
     1741 gagcgcgcgt cggccctgtg cgagcatgac gtagggacgg cggctttccg gctcggtgag
     1801 ccagcgggcg acggttccgt cggcgggcgc cgcgtccgag cgtagaggcg tgagacgcgg
     1861 gtcgaaggcg ctgatggttt cgttgggcat gtcgatgcgg tagcccagcg gcgagaatga
     1921 ttcggtcggg tcgaggacac catcgacacc tgcgggcagg ccgtcggcga tgatcgtgaa
     1981 gccctgcgag ggtaggagat ttccgcgtgc gccgagggcg aagttgcgaa cgtgtgcctt
     2041 gcgcgcccct tgtgcgccga cgccgctcag gccgacgacg cggacagcgc cgccgcccgt
     2101 ctcgacctcc acacgcgccg ccgacgccac gcgccgcgag aggatgttcg cccccgcgcc
     2161 cgtatcgacc gcgaactcga acggccccgt gccattaacc cacgtctgca ccaaaagccc
     2221 gcggccgtcc gcctcgtgca gacgcgcggg cgcgggtaga ccgatgcccg cgccatcccg
     2281 ttgttggaaa gcttcggggg cgttcgaggc gaagtggagt gtggacaaaa agcggtcgta
     2341 gacggacgaa tgggcagccc cgctcgcgag cggggctgcc cattgaatcg tcaacaggag
     2401 cgagagaacg accggaatcg cgaggactct gtttttcacg aggccgtctc tcatgtcttc
     2461 ggacaaggcg cgcgcttagt agcgtcggtc gcgcttgcgg agcttgtagg tatagagggc
     2521 ggagccgccg ccgccggcca gcgcgccgat gcccgcaccc ttcttaccac cgatcagacc
     2581 gccgatgagc gcgccgccgc cggcgcccat cgccaccgtc agcttgtcgc ggtgcttctg
     2641 ccagaaggaa cgcttctgcg gctggttgta gtcgtagtag acctgacgcg agttgccggc
     2701 gctgcgcgag cggacgtagc gcgtgcgcgg cgcagagcgg taggcaacgc gctcggtcgt
     2761 cccggcgcgc gtgggcgcgg cgacggccga cgcgctctca agaccttcct cacgggcggc
     2821 gcggaaaccc tcggcgaacc cttcgcggta gcactcgttc tccgtgttgt tcgcgggcag
     2881 agcctgcgtg tcggcggtgg tcgaggccgg gatgaagttc ttcgcgggcg ccgtggcggt
     2941 ggacggggtg gtgctgacgg ctgaggcgac ctgcgtctcg gccgacttcg tggggcgcat
     3001 gacgaacgcg ccaacgctcc cacccagaag ggctgcgccg agcgcgaccg ggactaactt
     3061 attcctcaaa atcattttca ggttctcctt cgggttgaaa agaaaaaatc gatgctggca
     3121 cacatccgtc tggcaagcgc cgttcctcac gcgtctccca cggaacatct cggtggacgc
     3181 agttaaagat gagtcgagtt taatcgaatt tgccttgaat acgaactatg tcgccgattt
     3241 agaagaatgc aacgagatcg caacacgagt gacgccgcgg tcggttatac gaaatgggaa
     3301 accgcgggac accatatcat caacgtgaag cccgcgcgcg cggcgtctgg cgtccacagg
     3361 agccaccggg ccggctcatt ggacggcccg gcgcgcgcgg gtttataatc gcacgcgctc
     3421 acccgaaaat tcgtggaggt cagtcgtccg aaccagtctt atgtctactt ccgtcttccg
     3481 ccttgcctcg cgtctcgacg ccgtcgggtt ttccgacatc gtcaaaatcc gcaaccgggt
     3541 gatgcagctt cgcgcgtccg gcgctcaggt cttccagttc gagggcggcg agccgttcct
     3601 gaacacgccc gaccccgtca aagaggcgat gatgcaggcg ctccgagaga accggacgcg
     3661 ctacgccccc tcgtccggca tccccgagct gcgtgcggcc atcgccgcca agctgcgtga
     3721 acgcaactgc atcgaggcga cggaagaaga cgtggtcgtc ttgaacggcg ggatgcaggg
     3781 attattcggg gcttttcaat cggtcgtcaa tcccggtgac gaggtgttgg tcttctcgcc
     3841 cttctggacg ccgatcaaag acctcatcgc acactgtcgc ggcgtggtcg tgctcgtgcc
     3901 gaccgaggag gcccgccgcg aaggcttccg cgaaacgctc gcccgctata gcaccgagcg
     3961 gacgcgcgcc gtctattaca acacgccgca gaacccgacc ggcgtcgtct acacgcgcga
     4021 cgaggccgag acagtggcgc gcttcgcgcg ggagcgcgac ctcgtcgtcc tcgccgacga
     4081 ggcttacgag gatttggtct acggcggcga gcacgtctcc atcgcctcgc tcgacgggat
     4141 ggccgagcgc acgatcactt ctttcaccct ctcgaagtct tatgcgatga cgggctggag
     4201 ggtcggatac gccgtggcga aagagccgtg gatggcgggg ctgaagaagt gcctgctcta
     4261 ctcgacgaac ggcgtctcca caccttcgca gtgggcggcg ctggccgcct tcgccctccc
     4321 cgaagatttt tatgtgacca tgcgccgcgc ttaccgcgag cgccgcgacc ttttgatctc
     4381 gggcctgtgt gagatcggcc tcgcctgcgc cgcgcccgcc ggggccttct acgccttccc
     4441 cgacgtgtcg agtatcaacc ccgacagccg cgttgcggcc gagacgctgc tcgagcgcgc
     4501 gcaggtcgcc accgtccccg gcatcgtctt cggcgagcac ggcgagggcc acctccgctt
     4561 ctccttctcc ctccccaaag agaccatcga agccggcctc gactcgatgc ggcgaaatct
     4621 ctgacgaagt gatgagtgat gagtgatgag tgatgagagg cgcgacgccg cgcgacgccg
     4681 gcgccggaag gattcctcac gaacggacac caacggcggc gagttcgatt tcatccgacg
     4741 cattcgtcaa gaagcattta acaggctcga cactcgccac gagcactcat cactcatcac
     4801 tcatcactc
//
LOCUS       DASVES010000523        11675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_470338, whole genome shotgun sequence.
ACCESSION   DASVES010000523 DASVES010000000
VERSION     DASVES010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470338"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VF240_22295"
     CDS             complement(<1..929)
                     /locus_tag="VF240_22295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7178006.1"
                     /translation="MTIRTTNAEEMIVFEGVSKFYGEILGVNRVNLSIAPGITSLVGP
                     NGSGKTTLMNLMTGLLRPTRGTISVLGTSPHDPEKLFRKVGYCSQFDSFPRGMTGREF
                     IEFYLSVHGYTRGEVRDLTQAALERVSMQDASERKVAGYSKGMRQRIRLAQSIAHNPS
                     VLILDEPLNGLDPMARAEIIRLFQSLAAEGLYLVISSHILHEVDMMSDSVVLMHNGYV
                     VAEGDVHGVRDEIEEHPMQILVRCDRPQVLAARLFEHEHTIEARIHNDRHGLFVKTRR
                     PDDFYLLLNRLVTENHLQVESVAPADDDLNAVY"
     gene            complement(926..1846)
                     /locus_tag="VF240_22300"
     CDS             complement(926..1846)
                     /locus_tag="VF240_22300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178007.1"
                     /translation="MAVYENTYGRYEDKLTPEWSRFLVLPRYALRDVFGSKLLTAIFC
                     LCFIYPLVAAILIYLHHNSNAIALLQIDLRELLPINASFFQYFVEVQCWFAFILTVLV
                     GPVLVSRDLANNGLPLYLCRPFSRAEYVLGKMTVLIVLLSLVTWVPGLLLFLLQAYLE
                     GARWIVSNSRIAGAIFVGSMTWIVLLSLLALALSAWVRWRVVASGALLGLFFIPSALG
                     EIVNELFHTRLGHLISPASLMKKIWRGLFGHFTREIGSFNIEDAWGRSFTNVVLREPP
                     LWACWLVIALVCLACLWLLSRKVRAYEVIK"
     gene            complement(1833..2795)
                     /locus_tag="VF240_22305"
     CDS             complement(1833..2795)
                     /locus_tag="VF240_22305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7178008.1"
                     /translation="MNPVIELNGLSVTFGRRAILKDLSGSVKGRAVGLLGPNGAGKTT
                     LIHTLLGFHAPSSGTVRILGEDIATNAKSLRAAVGYMPERDSFIAKMSCVRFVRLMAE
                     LSGVPSKAALERAHEALFYVGLGEARYRSVDTYSLGMKQLAKLAQAIVHGPRLIILDE
                     PTNGLDPPTRARMIRLIQEIRDSGRARILLSSHLLRDVEECCDEILILKDGRIAANCN
                     LDEERKANRRFIELETRGGDRAAFAEAIGGLGCEYALSGERRIKLVMQDDVEIDDLYR
                     LAFERQIQIRRLNYKRDSLEDIFLKAMEGEENGAAKGGNGDGRI"
     gene            complement(2957..5668)
                     /locus_tag="VF240_22310"
     CDS             complement(2957..5668)
                     /locus_tag="VF240_22310"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX7178009.1"
                     /translation="MKRNRKELNDMLDKGVTGIRDERVDQSAMKTAASRVWARVSEQA
                     AGEKLIDSPAAVAAAESIGGCADYQSLIPSYLRGELSPARALLLEDHRHECIPCRKAL
                     KSARTGAPQVAAQVKSAPTKQTSFAPVWRWSIAAGLLLCFGLAALFFVERLDLSGSAL
                     AATVESADGDLYRVSETESRALRTGEQIQKGERVRTSKDSNAVLRLADGSLVEMRERS
                     EVSITENMKGVTVKLERGDVAVEAAKQREGHLYVATPDSLVSVTGTIFFVGSGTKGSR
                     VSVVEGEVHVNHAGKDDVLHPGDQVTTNARLEAVPLTHEVAWSRNAARYTQLAAELTR
                     LRQDLNARVPRPGVRYTSRFLDLVPEGTVLYAALPNLSQRLADSHRIMQERINQNPAL
                     KEWWKNEGREGQGPGADRIIERVREFGSYLGDEIVVSAGLNAKNEPGNLLVLGELKNA
                     AGFRPYLEGQAAQFAREGRNGASIRIVDDPLAAATQSNAAPGKDELFIWVRNDLFAAS
                     PQIENLRQLATTLNNTGANRFVGTSFHARLAEIYREGAGLVVAANLEKLVGEAAREDG
                     NEPGGAARVEGFRQLGLMSLKHLVVEQKDLNEKTQSRAVLTFSEPRRGIASWLAAPGA
                     MGSLEFISPDANVATAFVVKEPALLVDDLLGFLETVQPDLRRQLREIEAQQGIDIRRD
                     LAAPLGGEFAFAIDGPVLPNPSWKMILEVNDQAHLQGSLERVVEKLNVLVALSGKGGL
                     RWENTTEGGRTIYTLRSADFGLEVNYTFVNGYLVVTPGRALLDRALKYRESGNTLLRS
                     PRFRETMPEDGNANFSAILYHNLAPALRPLAENAPESQRGALAAAAEMPPTLAYAYAQ
                     GDRITLAANTEGGPFGLSPDSLLGLPNAFAMQNILMNAMGEKDEQKR"
     gene            complement(5665..6252)
                     /locus_tag="VF240_22315"
     CDS             complement(5665..6252)
                     /locus_tag="VF240_22315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7178010.1"
                     /translation="MKENDLQSNAATTTRLRAVPAAEGAGELERLFRDHHEQVFRAAY
                     RITGSATDAEDVLQTIFLRLASNAGTPDLAPSPGGYLHRAAVNAALDLVRKRGRARLV
                     SFDSMESAQELNDPNFSPEEEHVDRELRALLRHAIAKLGERAANVFALRYLEGYDNNK
                     IAELLGTSPMVVAVTLHRARTRLRREIGQFLEKQS"
     gene            complement(6670..6743)
                     /locus_tag="VF240_22320"
     tRNA            complement(6670..6743)
                     /locus_tag="VF240_22320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6707..6709),aa:Pro,seq:cgg)
     gene            complement(6790..7491)
                     /locus_tag="VF240_22325"
     CDS             complement(6790..7491)
                     /locus_tag="VF240_22325"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7178011.1"
                     /translation="MESLTELFHQLRELLNPKNIIDFMTTKGLMFTYAGLVFIIFAET
                     GLAVGFFLPGDSLLVVAGLVARAGRLNVAVLLFSLFIAAVVGDAVGYYSGLKLGPRLF
                     ARKKSLLFRPSHLQKAHEFYEKYGGKTIIIARFVPIVRTFAPIVAGAAQMPYRRFLAY
                     NVVGGFAWVFSMILAGYFLGTVVENAFSIKLEDHIEKVVIVVVALSLLPPVIEYLKAR
                     REKKLRAGEAGDAQV"
     gene            complement(7580..8248)
                     /locus_tag="VF240_22330"
     CDS             complement(7580..8248)
                     /locus_tag="VF240_22330"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX7178012.1"
                     /translation="MSNPQSAQLRIPRERMVERLRDHYHIRDPRALDAMRAVPRHLFV
                     PEALQGRAYGDHALPIAANQTISQPYIVARMTELLEVDRDSRVLEIGAGSGYQTAVLA
                     RVAGQVYAIERIAELAREAQARIRSLGIYNATVKCFDGTFGWSAHGPYDAILVAAGSP
                     EVPEPLLAQLKVGGRLVLPVGATRESQQLIRVVRKEEGYETEEHGPCAFVPLIGRYGW
                     NEGT"
     gene            complement(8313..9110)
                     /gene="surE"
                     /locus_tag="VF240_22335"
     CDS             complement(8313..9110)
                     /gene="surE"
                     /locus_tag="VF240_22335"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX7178013.1"
                     /translation="MFRILLTNDDGIHSDGLTKLEEALREVGDVYTVAPASEMSGASH
                     SLTLSRPLRIRHIDRRHWTVDGTPTDCVTLALHRILPEEEHPHICVSGINHGGNLGDD
                     ASYSGTVAGALEATILGVPGIAFSLVAREHFDFTEAARFAVVAVRKALNEGLPEGTLL
                     NVNVPAGPLKGVRVTRQGIKNARPIISEHIDPRGKAYYWIGEEYYSSTFEDGTDYYAV
                     EHGYISVTPLRSDMTNHAELPTLAAWNLIGADGAGVGEDEDSPVSRA"
     gene            complement(9317..11356)
                     /locus_tag="VF240_22340"
     CDS             complement(9317..11356)
                     /locus_tag="VF240_22340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7178014.1"
                     /translation="MKPIKYAEKGLTAVAGGLFNTVQFFNQYRPGKPFTPKWSDKPIL
                     KSWQKSKPPLGFPRTTDSLCPKCVIEARQRILSMEVIDPSELINEKIGEIKAQIIERD
                     GQIWMVKDCPTHGHFEDIMAIDSKFLKHIETMFPGRDIQAHNDEKLHNHGSSSIKFGR
                     GSVLTIDLTNRCNMMCDPCFMDANQVGFVHELSFEEIQEILDKAISIKPRRQMSVQFS
                     GGEPTISPYFLDSIKYSRKVGYNSVQCATNGIEFAKSKEFCREAAAAGLRYAYLQFDG
                     IGNDANAHRQVGNLFDVKLRAIDNLHEAGVEIVLVITIVNGINNDQVGSVIRFALENP
                     KKIAFLSFQPVSFTGRDEEITDERRMQQRYTLSHLAQDVKKQVGITEPTRDWFPLSLM
                     GAFADFADLAHGPDAEWGQVSCGCHPNCGVGTAVMIDKETKEMAPVPEFLNIPGLVHD
                     MKRITDAGRGKWASNFMMGLTLLKHYDPYKAPSQFSLYELFKKFDKSFGLTGKTYGRV
                     DGKRTEEDIKARRSDRWNFLFIAGMWFQDLFNYDFRRTEMCIIPYGTQEGEISFCAYN
                     TGIGWRNIIEQMHQNATVAQWYKENGRHEIFAKGKEVGMEFEKPVNLVLNEVDLQRPH
                     KPEMEGPKTAAEEAAMMRRLYQEMVLKQKLGASDVEKNKPVQIQGLSRSKESANA"
     gene            11392..>11675
                     /locus_tag="VF240_22345"
     CDS             11392..>11675
                     /locus_tag="VF240_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178015.1"
                     /translation="MEYRAAQHKHTKFIKTVLMPRSLEFASLILLLPDRKALKRKRPF
                     AGRFIQNREHYAPLVQKASAYECLKATAHTSLKTFVPSLSPNSLVILLPL"
BASE COUNT         2127 a         3597 c         3712 g         2239 t
ORIGIN      
        1 tggtaaacgg cattcaagtc atcgtccgcc ggggcgaccg attcgacctg aagatgattc
       61 tccgtcacga gccggttgag caggaggtag aagtcatctg gccggcgcgt cttgacgaac
      121 agcccgtgcc ggtcgttgtg gatgcgcgcc tcgatggtgt gctcgtgctc gaagaggcgc
      181 gcggctaaaa cttgcgggcg gtcgcagcgc acgaggattt gcatcgggtg ctcctcgatc
      241 tcgtcgcgca cgccgtgcac gtcgccctcc gcgacgacgt agccgttgtg catcagcacc
      301 acgctgtcag acatcatgtc aacttcgtgc aggatgtgac ttgagatgac gaggtacaag
      361 ccttccgccg cgagcgactg aaacaagcgg atgatctcgg cgcgcgccat cgggtcgagt
      421 ccgttcaacg gctcgtcgag gatgagcacg gaagggttat gggcaatcga ctgcgcgagc
      481 cggatgcgct gcctcatacc tttgctgtag cccgccacct tgcgttcgga cgcatcctgc
      541 atcgacacgc gttcgagcgc cgcctgcgtt aaatcccgca cctcgcctcg cgtgtagccg
      601 tgaacgctca ggtagaactc gatgaactcg cgccccgtca tgccgcgcgg gaaggaatcg
      661 aactgcgagc agtagccgac tttgcggaag agtttttccg ggtcgtgcgg gcttgtgccg
      721 agcacgctga tcgtcccgcg cgtcggtcgc agcagccccg tcatcaggtt catcagcgtc
      781 gtcttgcctg aaccgttcgg gccgacgagg ctcgtgatgc ccggcgcgat ggagaggttg
      841 acgcggttga cgccgagtat ctcaccgtag aattttgaga cgccctcgaa gacgatcatc
      901 tcctcggcgt tcgttgtccg tatcgtcatt tgatcacttc gtaagctcgg actttgcgcg
      961 agagcagcca gaggcaggcg aggcagacca gagcgatcac cagccagcag gcccacagcg
     1021 gcggctcgcg caacacgacg ttggtgaagc tccggcccca ggcgtcctcg atgttaaagc
     1081 tgcctatctc ccgcgtgaag tggccgaaga gcccgcgcca gatcttcttc atcaaggacg
     1141 cggggctgat caggtgtccg aggcgcgtgt ggaaaagttc gttgacgatc tcgccgaggg
     1201 cggacgggat gaagaacaat ccgagcagcg cgccgctcgc cacgacgcgc caacgcaccc
     1261 acgccgagag cgccagcgca agcagcgaga gcagcacgat ccacgtcatg ctcccgacga
     1321 agatcgcccc cgcaatccgc gaattcgaca cgatccagcg cgcgccttcg agatacgctt
     1381 gcaggagaaa gagcaggagt cccggcaccc acgtcacaag cgacagcaaa acaatgagca
     1441 ccgtcatctt gccgagcacg tattcggcgc gcgagaacgg gcggcacagg tagagcggca
     1501 agccgttatt cgccagatcg cgcgagacga gcacggggcc gacgagcacc gtgaggatga
     1561 aggcgaacca gcactgaact tcgacgaagt actggaagaa cgatgcgttg atcggcaaca
     1621 actcgcgcaa gtcaatttgc aggagcgcga tggcattcga gttatgatgt aagtagatca
     1681 ggatcgccgc gacgagcggg tagatgaagc acaggcagaa gatcgcggtc aagagcttcg
     1741 agccgaacac gtcgcgcagc gcgtatcgcg gcaggacgag aaagcgcgac cactccggcg
     1801 tgagcttgtc ttcgtaccgc ccgtaggtgt tttcatatac ggccatctcc gttccctcct
     1861 ttggccgcgc cgttctcttc gccttccatc gccttgagaa aaatgtcttc gagcgaatcg
     1921 cgcttgtagt tcaagcggcg aatctgaatc tgccgctcga aggctaaacg ataaagatcg
     1981 tcgatctcca cgtcgtcctg catcacgagt ttgatgcgcc gctcgccgga gagcgcgtac
     2041 tcgcagccta atccgccgat ggcttcggcg aacgccgcgc ggtcgcctcc gcgcgtctcc
     2101 agttcgataa acctgcggtt cgctttgcgc tcttcatcaa ggttgcagtt ggcggcaatg
     2161 cgcccgtcct tcaagatgag aatttcgtcg cagcattcct caacgtcgcg caacagatgt
     2221 gacgagagca ggatgcgcgc ccggccgctg tcgcgtatct cctgtatcag gcggatcatg
     2281 cgcgcccgcg tcggcgggtc aagcccgttc gtcggctcgt ccaaaatgat caaacgcggc
     2341 ccgtgcacga tagcttgcgc gagtttagcg agttgcttca tgccgagcga ataggtgtcc
     2401 accgagcggt agcgcgcctc gcccaggccc acgtagaaga gggcttcgtg cgcgcgttcg
     2461 agcgccgctt tggaagggac gccggagagt tcggccatca gtcggacaaa gcgaacgcaa
     2521 gacattttgg cgatgaacga atcgcgctcc ggcatgtagc cgacggcggc gcggaggctc
     2581 ttggcgttcg tggcgatgtc ttctccgagg atgcgtaccg tgccggagct gggcgcgtga
     2641 aagccgagca gcgtgtggat gagcgtcgtc ttgcccgcgc cgttcgggcc gagcagaccg
     2701 acggcgcgac ccttgaccga cccgcttaaa tccttcagga tcgctcgcct gccgaacgtg
     2761 acacttaaac cgttgagttc gataacaggg ttcataagct gatgagactc cctacgatct
     2821 caagcgtaag ggagtttaca taaaattgtg cgggcagagg actcatcctc tgcccgcgcg
     2881 gttcgctgtt aaagcaggca ggggaactcg gtcccctgcc tgtaccccct tgatcatcgc
     2941 ccagagtttc ccgacttcaa cgcttctgct cgtccttctc gcccatcgcg ttcatcagga
     3001 tgttttgcat cgcaaaggcg ttcggcagac cgagcaggct gtcggggctt aacccgaacg
     3061 gcccgccttc ggtgttcgcc gcgagcgtga tgcggtcgcc ctgcgcgtaa gcgtaggcca
     3121 gagtcggcgg catctcggcg gcggcggcga gcgccccgcg ctgactctca ggcgcgttct
     3181 ccgccagcgg gcgaagggcg ggcgcgaggt tgtggtagag aatcgcggag aagttagcgt
     3241 taccgtcctc cggcatcgtc tctcggaagc gcggcgaacg caagagggtg ttgccggatt
     3301 cgcggtactt caaggcgcga tccagcagcg cgcggcccgg ggtgacgacg aggtagccgt
     3361 tcacaaaggt gtagttcacc tctagtccga agtcggccga acgcagggtg taaatcgtgc
     3421 gtccgccctc ggtcgtgttc tcccagcgca atccgccctt gccgctcagc gccaccaaaa
     3481 cgttcagctt ctcgaccacg cgctcaagcg acccctgcaa gtgcgcttga tcgttcacct
     3541 caaggatcat cttccaggag gggttcggga gcacggggcc gtcgatggcg aaggcgaact
     3601 cgccgcccag cggcgcggcc aggtcgcgcc ggatgtcgat tccctgctgc gcttcaatct
     3661 cgcgcagttg gcggcgcaga tcgggctgca cggtttccag gaagccgagc agatcgtcaa
     3721 ccagcaacgc gggttctttg acgacgaagg cggtcgccac gttcgcgtcc ggcgagatga
     3781 actcaagcga gcccatcgcc cccggcgcgg cgagccacga ggcgatcccg cggcgcggct
     3841 cgctgaaggt gagcacggcg cggctctgcg tcttctcgtt caaatccttc tgctcgacga
     3901 cgaggtgttt caggctcatc agtccgagct ggcggaagcc ctcgacgcgc gctgcgccgc
     3961 ccggctcatt gccgtcctcg cgcgcggcct caccgacgag tttttccagg ttggcggcga
     4021 ccaccagccc cgcgccctcg cggtagattt cagcaagacg cgcgtggaag gacgttccca
     4081 cgaagcggtt cgcgccggtg ttgttgagcg tcgtcgcaag ctgacgcaaa ttttcaatct
     4141 gcggcgacgc ggcgaaaagg tcgttgcgaa cccagatgaa gagttcatct tttccgggag
     4201 cggcgttcga ttgtgtggcg gccgcgagcg gatcgtcaac tatgcgaatc gacgcaccgt
     4261 tcctgccctc gcgggcgaac tgggcggcct gcccttcaag gtacgggcgg aagccggccg
     4321 cgttcttcaa ctcaccgaga acaaggaggt tgcccggctc gtttttcgca ttcaggcccg
     4381 cgctgacgac gatctcgtcg ccgaggtacg agccgaactc gcgcacgcgt tcaatgatcc
     4441 gatcagcccc cgggccttgc ccttcgcgcc cttcgttttt ccaccactct ttgagcgcgg
     4501 gattctggtt gatgcgctcc tgcatgatgc ggtgtgagtc ggcgagtctc tggctgagat
     4561 ttgggagcgc ggcgtaaagg acagtgcctt cgggcacgag gtcgaggaag cgcgaggtgt
     4621 agcgcacgcc ggggcgcgga actctcgcgt taaggtcctg ccgcagcctc gtcaactccg
     4681 cggcgagctg cgtgtagcgc gcggcgttgc ggctccaggc aacttcgtgc gtgagcggga
     4741 cggcttcgag gcgcgcgttg gtcgtcacct gatcgccggg atgcaataca tcgtctttgc
     4801 cggcatggtt gacatgcact tcgccttcga cgaccgagac gcgcgacccc ttcgtgccgc
     4861 tgccgacgaa gaagatcgtg cccgtcacgg agacgagcga atccggcgtg gcgacgtaga
     4921 gatggccttc gcgctgcttc gccgcctcga ccgccacgtc gccgcgttcg agtttgacgg
     4981 tgacgccctt catgttctcg gtgatcgaca cctcgctgcg ctcgcgcatc tcgacgagcg
     5041 atccgtccgc gaggcgcaac acggcgtttg aatctttgga cgttcgcacg cgctcgccct
     5101 tctgaatctg ttcgcccgtg cggagcgcgc ggctctccgt ttcggaaacg cgataaagat
     5161 cgccgtccgc gctctcgacg gttgcggcga gcgcactgcc ggagagatcg aggcgctcga
     5221 cgaagaagag cgcggcgagt ccgaagcaca gcagcagacc ggcggcgatg ctccagcgcc
     5281 acaccggggc gaacgacgtc tgcttcgtcg gcgcggattt aacttgcgcc gcgacttgcg
     5341 gcgcgcccgt gcgcgccgac ttcaaagcct tgcggcaagg gatgcactcg tgcctgtgat
     5401 cttcgagcag cagagcgcgc gccggcgaaa gctctccgcg aaggtaagaa gggatgagcg
     5461 attggtagtc ggcgcagccg ccaatagatt cggcggcggc gacggcggcc ggggagtcaa
     5521 tcaacttctc gcccgccgct tgctcggaga cgcgcgccca gacgcgcgag gccgccgtct
     5581 tcatcgccga ctgatcgacg cgctcgtcgc ggatgccggt cacacctttg tctaacatgt
     5641 cgttaagctc ttttctgttg cgcttcatga ttgtttctcc aaaaactgtc caatctcacg
     5701 gcgaaggcgc gtgcgcgcgc ggtggagtgt gacggcgacg accatcggcg aagtgccgag
     5761 aagttcggcg attttgttgt tgtcgtagcc ttcgaggtag cgcagcgcga agacgttcgc
     5821 ggcgcgctcg ccgagtttcg caatcgcatg gcgcaagagc gcgcgcaact cgcggtcgac
     5881 gtgctcctcc tccgggctga agttcgggtc gttcaactcc tgcgcggatt ccatcgaatc
     5941 gaacgaaacc agacgcgcgc gcccgcgctt gcgcacaagg tcgagcgcgg cgttgacggc
     6001 ggcgcggtgc aggtaacccc ccggactcgg cgcgagatca ggcgtcccgg cgttcgaggc
     6061 cagacgtaaa aagatcgtct ggaggacgtc ctccgcgtcc gtggcacttc ccgtaatgcg
     6121 atacgcggcg cggaaaacct gctcgtggtg atcgcggaag agtctctcaa gttcgcccgc
     6181 cccctccgct gcggggacgg cccttaagcg ggtcgtagta gctgcatttg attgcaaatc
     6241 gttctccttc acaccgtcac tacgcatata acgcgccccg cagcggatta ttaacaacac
     6301 tgaaaaattt attttcgtgc ttcgtaaaga acatgaacgc cgacggcgtc acccaaaggc
     6361 gccgaaacgg ccggggccag ggcgccggcc gcgagcgccg acggcgccgg cgcggcggga
     6421 ctggacgtga acggcaaggc gggactgttc gtcagcgaga gcaccggcga gccgctgcgc
     6481 ctgattgcga acaagggcga gctacgcatc cccggcgcac gctccgccct catggcggta
     6541 accaagcacc gtttccgcca tccgcgcggc gatttgtttt tcatgtcgca ggacgagttc
     6601 gagttgaact tcctaccaca aatgaaaaca gcaaccattg ctggttgctg taaatcgtta
     6661 tatctaaaat cggggcgaga ggattcgaac ctccgacctc tcggtcccga accgagcgct
     6721 ctaccaggct gagccacgcc ccgtgcctca actgtcgagg agcattctaa gtgcgccgcc
     6781 tcgttcctgt caaacctgtg cgtcgccggc ctcgcccgcg cgcagtttct tttcccgccg
     6841 cgccttcaga tactcgatga ccggcgggag caatgacaac gcgacgacga cgatgacgac
     6901 cttctcgatg tggtcttcga gtttaatgct gaaggcgttc tcgaccaccg tgccgaggaa
     6961 gtagccggcg aggatcatgc tgaagaccca cgcgaagccg ccgacgacgt tgtaggcgag
     7021 gaagcgccgg tagggcatct gcgccgcgcc ggccacgatg ggcgcgaagg tgcggacgat
     7081 gggcacgaag cgcgcgatga tgatggtctt tccgccgtac ttctcgtaga actcgtgcgc
     7141 cttctgcaag tggctcgggc ggaagagtag cgacttcttg cgcgcgaaga gacgcgggcc
     7201 aagcttgagg cccgagtagt agccgacggc gtcgccgacg accgccgcga tgaataggct
     7261 aaaaagcagg acagcaacat tcaaacggcc cgcgcgcgcc accagtccgg cgacgacgag
     7321 cagcgagtcg cccggcagga agaagccgac ggcgaggccc gtctcggcga agatgatgaa
     7381 gaccagcccc gcgtaggtga acatcaaccc cttcgtcgtc atgaagtcga tgatgttctt
     7441 cgggttgagt agttccctga gttgatgaaa tagttctgtc agtgattcca tattaaaaac
     7501 agaggtcaga ggttagaggt tagaggtcag tgaaagacgg attggttttt attgacctct
     7561 aacctctagc ctctgacctc tatgttccct cgttccagcc gtagcggccg atgagcggga
     7621 cgaaggcgca ggggccgtgc tcctccgtct cgtagccttc ttctttcctg acgacgcgga
     7681 tgagttgctg cgactcgcgc gtggccccca cgggaagcac aagccgtccg ccgaccttca
     7741 actgcgccag cagcggctcg ggcacctcgg ggctgccggc ggcgacgagt atcgcgtcgt
     7801 atgggccgtg ggcgctccag ccgaacgtgc cgtcgaagca tttcaccgtc gcgttgtaaa
     7861 tcccgagcga gcggatgcgc gcctgcgcct cgcgcgccag ttccgcgatg cgctcgatgg
     7921 cgtagacctg tccggccact ctcgccagca ccgccgtctg gtagcccgag ccggcgccga
     7981 tctccaggac gcggctgtcc ctgtccacct ccagcagctc cgtcatgcgg gcgacgatat
     8041 agggctgcga aatggtctgg ttggcggcga tggggagcgc gtggtcgccg taggcgcggc
     8101 cctgtagggc ctcgggcacg aagaggtggc gcggcacggc gcgcatcgcg tcgagggcgc
     8161 gcgggtcgcg gatgtggtag tggtcgcgca ggcgctcgac catccgctcg cgcgggatgc
     8221 gtaactgtgc ggactgcgga ttagacaaac cgaaaaacct tttgactgca acgggccgcg
     8281 cccgaaagcg gatgcttctt gcgcgggaag actcaggcgc ggctgacggg gctgtcttcg
     8341 tcctcgccta ctccagcgcc gtcggcgccg atgaggttcc aggcggcgag cgtgggcagc
     8401 tcggcgtggt tcgtcatgtc gctgcggagc ggagtgacgg agatgtatcc gtgctcgacg
     8461 gcgtagtagt ccgtgccgtc ctcgaaggtg gagctgtagt actcctcgcc aatccagtaa
     8521 tacgccttgc cgcgcgggtc gatgtgctcg ctgatgatcg gccgcgcgtt cttaattccc
     8581 tgccgcgtca cgcgcacgcc cttgagcggc ccggcgggga cgttgacgtt gaggagcgtc
     8641 ccctcgggga ggccctcgtt cagcgccttg cggacggcga cgacggcgaa acgcgccgcc
     8701 tccgtgaagt cgaagtgctc gcgcgcgacg aggctgaagg cgataccggg gacgccgaga
     8761 atcgtcgcct cgagcgcccc ggcgacggtg cccgagtacg acgcgtcgtc gccgaggttc
     8821 ccgccgtggt tgatgccgga gacgcagatg tgcggatgtt cttcctcggg gaggatgcgg
     8881 tggagcgcga gcgtcacaca atcagtcggg gtgccgtcca ccgtccagtg gcgtcggtcg
     8941 atgtggcgga tacgcagggg ccgcgagagc gtgagactgt gggaggcgcc gctcatctcg
     9001 gaggccgggg ccaccgtgta aacgtcgcct acctcgcgca gcgcctcttc gagcttcgtc
     9061 aggccgtcag aatgaatccc gtcgtcgttc gtcagaagaa tacgaaacat aaaattaaag
     9121 ccgaacccat tacagcttaa cgccgcgaat tattcaagca agcggccgtc accggttcat
     9181 ttccagcggt ctgttgtgcg gacgatattt taaaccgact ctcatcttat taccgcgccg
     9241 gccgcaaacg aaagccccgc gaccgtccga gcaacgcgtc gcggggcttt tctcctcgaa
     9301 gccttcggcc gtcagtttag gcgttagcgg actccttgct ccgcgacagg ccctgaatct
     9361 ggacgggctt gttcttctcc acgtccgagg cgccgagctt ctgcttcaga accatctcct
     9421 gatagagacg gcgcatcatc gcagcctcct cggcggccgt cttcggccct tccatctccg
     9481 gcttgtgcgg gcgctggagg tcaacttcgt tgaggacgag gttgaccggc ttctcaaact
     9541 ccatcccaac ttccttaccc ttggcgaaaa tctcgtggcg gccgttctcc ttgtaccact
     9601 gcgcgacggt cgcgttctgg tgcatctgct cgatgatgtt gcgccagccg atcccggtgt
     9661 tgtaggcgca gaaggagatt tcgccctcct gcgtgccgta ggggatgatg cacatctcgg
     9721 tgcggcggaa gtcgtagttg aataagtcct ggaaccacat gccggcgatg aagaggaagt
     9781 tccagcggtc ggagcggcgc gccttgatgt cttcctcggt gcgcttgccg tccacgcggc
     9841 cgtaggtctt gccggtgagt ccgaacgact tgtcgaactt cttgaacagc tcatagagcg
     9901 agaactgcga cggagccttg tagggatcgt agtgcttgag cagcgtcagc cccatcatga
     9961 agttcgacgc ccacttgccg cggccggcgt ccgtgatcct cttcatgtcg tgtacgaggc
    10021 cggggatgtt caggaactcc ggcaccggcg ccatctcctt cgtctccttg tcgatcatca
    10081 cggccgtgcc cacgccgcaa ttcgggtggc agccgcagga gacctgtccc cactcggcat
    10141 cggggccgtg cgcgaggtcg gcgaagtcgg cgaaagcccc catcaagctc aaggggaacc
    10201 agtcgcgcgt cggctcggtg atgccgacct gctttttaac gtcctgcgcg aggtgcgaca
    10261 gggtgtagcg ctgctgcatg cggcgctcgt cggtgatctc ctcgtcgcga cccgtgaagg
    10321 agacgggctg gaaggagagg aaggcgatct tcttcgggtt ctccagcgcg aagcggatga
    10381 cggagccgac ctgatcgttg ttgatgccgt tgacaatcgt gatgacgagc acgatctcga
    10441 cgcccgcctc gtgcaggtta tcgatggccc gcagcttcac gtcgaagagg ttgccgacct
    10501 ggcggtgcgc gttggcgtcg ttgccgatgc cgtcgaactg gagataggcg tagcgcaggc
    10561 cggcggccgc cgcctcgcgg cagaactctt tggacttcgc gaactcgatg ccgttcgtcg
    10621 cgcactggac ggagttgtag ccgaccttac gcgagtactt gatggagtcg aggaagtagg
    10681 gcgagatggt cggctcgccg ccggagaact gcaccgacat ctggcggcgc ggcttgatcg
    10741 agatggcctt gtcgagaatc tcctgaatct cctcgaacga caactcgtgg acaaagccga
    10801 cctggttggc gtccatgaag cacgggtcgc acatcatgtt gcagcggttc gtcaggtcga
    10861 tggtcagcac cgacccgcgg ccgaacttga tggacgagga gccgtggttg tggagcttct
    10921 cgtcgttgtg agcctgaatg tcgcgccccg gaaacatcgt ctcgatgtgc ttgaggaact
    10981 tcgagtcaat cgccatgatg tcctcgaagt ggccgtgggt ggggcagtcc ttgaccatcc
    11041 agatttggcc gtcgcgctcg atgatctgcg ccttgatctc gccgattttc tcgttgatga
    11101 gttcggaggg gtcgatcacc tccatgctca aaatcctctg gcgcgcctcg ataacgcact
    11161 tcgggcagag cgagtcggtc gtgcgtggga agcccagcgg cggcttcgac ttttgccacg
    11221 acttgaggat cggcttgtca gaccacttgg gcgtgaaagg cttgccgggc cggtactggt
    11281 tgaagaactg gacggtgttg aagagaccgc ccgcgacggc cgtcagcccc ttctcagcgt
    11341 acttaatcgg tttcattctt tgttccttct ctcgttttcc gcaaaggccc gttggagtac
    11401 cgcgcggctc aacataaaca cacgaaattc atcaagacgg ttttgatgcc gcgctctctc
    11461 gaattcgcct ctttaattct cttgctgcca gacaggaagg ctctgaagcg taaacgccct
    11521 tttgccgggc ggttcattca aaaccgagaa cattacgcgc cgctagtaca gaaagcaagc
    11581 gcctatgaat gcctgaaggc gacagcacac acgtcactca agacgtttgt accgtcgctc
    11641 tcaccaaact cccttgtaat cctgctgccg ctaaa
//
LOCUS       DASVES010000524         5460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_705093, whole genome shotgun sequence.
ACCESSION   DASVES010000524 DASVES010000000
VERSION     DASVES010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705093"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..607)
                     /locus_tag="VF240_22350"
     CDS             complement(83..607)
                     /locus_tag="VF240_22350"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX7178016.1"
                     /translation="MSAPIITTAILLACLSIAIIYTDTRYRRIPNKLVLATLVLGLTV
                     NSIFGGFQGLLSSLGGFALAFVVMLLFHAFGAMGAGAVKLFAAVGSVVGLSHVLPALL
                     VVVLTGGLLALCKMIYSGTVKTTMLNVLQFFVSVCSGQKPPRFEATADRRFTIPYGVA
                     ICVGSLVSLFVFRA"
     gene            complement(717..875)
                     /locus_tag="VF240_22355"
     CDS             complement(717..875)
                     /locus_tag="VF240_22355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178017.1"
                     /translation="MQRIKNFLRDESGMETLEYAAIAGLVAAAALLVYGSAWGTQLET
                     RLLDAVTP"
     gene            complement(1602..2069)
                     /locus_tag="VF240_22360"
     CDS             complement(1602..2069)
                     /locus_tag="VF240_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178018.1"
                     /translation="MKRLSAQHAFAAVLLLALTAAGALTVSAQEDGAEPASPIILENK
                     HVMMMVRAKIAPEVILEKIRISSCNFDTFPPLLAELKSKGVPDSVLLAMVKAPHGVSA
                     AEADARGPVVYHPVDEVLKYTAAYSRTGSAVTPSRAARPSTRRSAPAPIRRRG"
     gene            2614..3114
                     /locus_tag="VF240_22365"
     CDS             2614..3114
                     /locus_tag="VF240_22365"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX7178019.1"
                     /translation="MTFALLLAAYTAAALLVHELGHLAAARFCRVPAKELALGLGPAL
                     LRVNLRGFTFSIRALPLGSFVRLDGSELSERTTGQKLLVHLGGVLSNALVGVLFLSHP
                     FGWLNLLIAAGNLLPLYQHDGWKCGLVLMRALVGRTSRPAEQLFTFSGGFISLVIILA
                     IIRLSS"
     gene            3177..3794
                     /locus_tag="VF240_22370"
     CDS             3177..3794
                     /locus_tag="VF240_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178020.1"
                     /translation="MKRCPSCGESYGDGVKFCDGDGTPLVAEFAPAEGRQTRWGALGV
                     GLALGVAVCVAAFVLYRVATRDDKGATVATSPSSTPVRDAPPPPVRHAAAEPDTPTPT
                     PSPEATPSLSPTPVPTPTPPKSEEDSLDILSNSPARTGGPEARADARVIFHLTDGASV
                     EAEDAWKAPGGAWYRRGGVLEWLERSRIRSVERRAAPTPAPSAPQ"
     gene            4042..>5460
                     /locus_tag="VF240_22375"
     CDS             4042..>5460
                     /locus_tag="VF240_22375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7178021.1"
                     /translation="MEDIGDTNILVVNDNPDHLELMRMALRKVGYGVLTALDGGEAFE
                     TALRERVELIISDVMMPDVNGIELCRLVRGHPALKSLPILLVSALRKDTESVVEGLRS
                     GADAFVESPFDLMHLIAVISRLVERKRGEDHILRLNEELEVRVRERTAQLESANLELE
                     HEVAERRVIESDLEKARDEALVTARLKAEFLANVSHEIRTPMHAVLGMVGLLLKTPLR
                     PKQAEFAEAIRESADSLLAIINDVLDLSKIEAGKMRVDKTDFDLSDIVDASVNMFLER
                     VRAKGLSLGCMIDDGVPTLLRGDPGRLRQVLVNLLSNAVKFTESGAVTLEVTGGPEAD
                     GHVTLRFEVKDTGIGISREARQRIFLPFTQADGSDTRPYGGTGLGLAISRQLVELMGG
                     EIGVESEAGRGSTFWFTVRCAQQPGAAPDAPRSDLEGLSLLVVGSDAAHRGVLLQQAG
                     QWKMPAAEAEDAGRALGLLREAQ"
BASE COUNT          986 a         1744 c         1784 g          946 t
ORIGIN      
        1 ggcgccggtg agtgcaatga ttagtcgttt gttgctgcgc ataactcaaa atctccgata
       61 ccgagggtcg gccgtaagcg cgtcaggcgc gaaagacgaa cagcgaaacg aggctgccga
      121 cgcagatagc cacgccgtaa ggaatcgtga accggcgatc cgccgtggcc tcaaaacgcg
      181 gcggcttctg gccggagcag acgctgacga agaattgcag aacgttgagc atcgtcgtct
      241 tgacggtgcc ggagtaaatc attttgcaga gggccaggag tccgccggtc agcaccacta
      301 ccaaaagagc cgggagcacg tgcgagagac cgacgaccga gccgacggcg gcaaaaagct
      361 taacggcgcc cgcgcccatc gcgccgaagg cgtggaacag gagcatcacg acgaaggcga
      421 gcgcgaaccc gccaaggctc gaaagtaaac cttgaaagcc gccgaagatg gagttaacag
      481 tgagccctaa gaccagcgtg gccagaacga gcttgttggg gatgcgacga tagcgcgtat
      541 ccgtgtaaat aatggcaatg gacagacagg ccagtaagat tgctgttgtg atgatggggg
      601 cagacattgc gtaattccga cggggacatc agggtgtctc ctcttggaag aaaggggtca
      661 gaggcgacgg gccagccact aaccggcccg tcgcctcgct tatgcgctgc ttaccactac
      721 ggggtgacgg cgtcgaggag gcgggtctcg agctgcgtgc cccaggccga gccgtagacg
      781 agcagcgcag ccgcggcgac caggccggcg atggctgcgt actcgagggt ttccataccg
      841 gactcgtcac gaaggaagtt cttgattctc tgcattgtct ttttctccta aatcccaatt
      901 tggggggtca agtttttgct gtcttagtcc tgcgtccttt cgcgcaccgc ctgggcgcag
      961 gacttcggac aacgggataa gtttcagggc gtttcaaccg acggcgccgt gtgcgtgacg
     1021 gcttctacga cactgagcga ggcgttgcgt gcgttcccct cgatacttca gtcatcaacg
     1081 aaacagattc agtcaccaag catgtgcggc ataatagcgg cgcgggggca atcgcgcatc
     1141 ggacaatgcg attattccgt gtgggcgctt acaaccattt ttcagggggg gagggggcag
     1201 aatgccctag taggtggggg cacatttgtg ccaagcgcac atccaatcca gtcggatggc
     1261 ggcgacttca atgtgggcga ccgccggcgg gattccaaca gaggacaggc tcgtgagctt
     1321 ttactttact attagccaat gccacgtgag cgtacccggc gcgcctgcac ggccgtgaag
     1381 gcatgttccc tgacgcgccc tcccgaacat gcttcggggc cgcagtcgag tgccgttccc
     1441 ggctcggagt gctcggcttc agaggggtag tggtagaggc tgaaacttca gttggggctt
     1501 attggcaggg ggaatttaga caccacttgc agcgtcgcgg ggagcattgg ggcgccgggg
     1561 ccatgttgac gcctattttg tgatgagcgg gtgggggcgc cttaccccct gcgcctgatc
     1621 ggcgcgggcg ccgacctcct cgtggacggt ctggcggcgc gcgacggcgt cactgctgac
     1681 cctgtcctgg agtaggcggc ggtgtacttc aacacctcgt ccacggggtg gtagacgacg
     1741 gggcctctcg cgtccgcctc ggcggcggac accccatgtg gcgccttgac catcgccaac
     1801 aggacggagt cgggcacgcc cttcgacttg agttcagcca ggagcggcgg gaaagtgtcg
     1861 aagttgcagg acgagatgcg gatcttctcg agaatgactt cgggggcgat cttcgccctc
     1921 accatcatca tgacatgttt gttctcgagg atgatcgggg aggcaggctc agcgccgtcc
     1981 tcctgcgcgg aaacggtcaa ggccccggcg gcggtcaggg ccaggagaag gacggcggcg
     2041 aatgcatgct gggccgagag gcgtttcatg ggggcgctcc tttcaattca gggcgcataa
     2101 gatataacag tcggcctcgt cgcacaatgc acaagcggcc caatttgcaa aagaaagtat
     2161 tgacacaagt gtgccggaga aggaacccga cgtggctctc ccggacatct tcctcaagca
     2221 cgagaagcgc actgacttcc tcccgcgggt ctttgcggca gacaaggggc taacacggct
     2281 gtaatctttt aacgcacgtg gccgggtccg ccggacggtc tttgcccccg caagagtcac
     2341 ataatctgtc agcgcccccg gcccgcttca cgtcaggcac tcagaaactc ctgtaaaatc
     2401 ccccgacttc tcccctgcga gcctgcgtcg cccggctggc acacgtcttg aatgcggcag
     2461 agccgttggc ctcccccacg ccttcaccga aagggggaag accgaccatg acactggctc
     2521 tttcgatact cttcttcttc aacttctaca cgagactccg cgacagcgag gtcgggaggt
     2581 aaagcggccc gcgccgccgc aaaggacacc catatgacct tcgctctcct gctcgccgcc
     2641 tacaccgccg ccgccctgct cgtccacgag ctcggccatc tggccgccgc gcgcttctgc
     2701 cgcgtccccg ccaaggagct tgcgctcggg cttgggcctg cgctgctgcg cgttaacctg
     2761 cgcggcttca ccttcagcat ccgcgccctc ccgctcggct ccttcgtgcg gctcgacggc
     2821 tctgagctga gtgagaggac gacggggcag aagctcctcg tccatctggg cggggtgctc
     2881 tcgaacgcgc tggtcggcgt gctcttcctc agccacccct tcggctggct caacctcctg
     2941 attgccgccg gcaacctcct gccgctctat cagcacgacg gctggaagtg cggcctcgtc
     3001 ctgatgcgcg cgctcgtggg gcggacgagc cgcccggcgg aacagttatt caccttctcg
     3061 ggcgggttta taagtctcgt catcatcctg gccatcatcc ggctaagctc ctaggctaat
     3121 cccaacgcac gggtcgtata gtcgccgggc gcgcggctgc tgttataatg gcggccatga
     3181 aacgttgccc ctcctgcggc gagtcgtacg gcgacggagt gaagttctgc gacggcgacg
     3241 ggacgccgct cgtcgccgag ttcgcgccgg ccgaagggcg ccagacgcgt tggggcgcgt
     3301 tgggcgtcgg gctggccctc ggggtggcgg tgtgcgtcgc ggccttcgtc ctctaccgcg
     3361 tcgccacgcg agatgataaa ggggccaccg tcgctacctc cccttcctcg acgcccgtcc
     3421 gtgacgcgcc gccgccgccg gtgcgccatg ccgcggcaga gccggacacc ccgaccccga
     3481 ccccgtcgcc ggaggcgacg ccgagtcttt cgccaacacc cgtcccgacg ccgacgccgc
     3541 ccaagagcga ggaggacagc ctcgacatcc tgagcaactc gccggcccgg acaggcggcc
     3601 ccgaagcgcg cgccgacgcc cgcgtgatat ttcatctgac tgacggcgcc agcgtcgaag
     3661 cggaggacgc gtggaaggcg ccgggcgggg cgtggtatcg ccggggcgga gttttggagt
     3721 ggctggagcg ttcccgcatc cgctccgtcg aacgccgcgc cgcgccgacg cccgctccct
     3781 cggccccaca gtgatcgtca cgctgaaaaa cgtgtggccc ggtttttctc gcgcgcccgc
     3841 cgaacgttcg gactgacgca gtagaaaacg gatccggccg gggcggggcc gcgcgccttt
     3901 tctttacagc cggggcgtca gcacaaaagc gctataataa atgcgtcggc actctgctgc
     3961 cgccggggga gtggccggtt cgggcttcac cgaaccgggc acattcgacg cgtatatttc
     4021 gtcggtgagg agtttcgcgc agtggaggac attggcgaca ccaacatcct tgtcgtaaac
     4081 gacaaccccg accatctgga gttgatgcgg atggcgctga ggaaggtcgg gtacggggtg
     4141 ctgaccgccc tcgacggggg cgaggccttc gagaccgcgc tccgcgagcg cgtcgaactc
     4201 atcatcagcg acgtgatgat gcccgacgtc aacggcatcg agctgtgccg cctcgtgcgg
     4261 gggcacccgg cgctgaagtc gctcccgatt ctcctcgtca gcgccctgcg caaagacacc
     4321 gaaagcgtcg tggagggcct ccgctcgggc gccgacgcct tcgtcgaaag cccgttcgac
     4381 ctcatgcacc tgatcgcggt catctcgcgc ctcgtcgaac gcaagcgcgg cgaagaccat
     4441 atcctccggc tgaacgagga gctggaggtg cgcgtcagag agcggaccgc gcaactcgaa
     4501 tcggccaacc tcgaactcga gcacgaagtc gccgagcgcc gggtgatcga gtctgatctg
     4561 gagaaggcgc gcgacgaggc cctcgtgacc gccagactga aggccgagtt tttggccaac
     4621 gtcagccacg agatccgcac ccccatgcac gccgtcctcg gcatggtggg gcttttgctg
     4681 aaaacccccc tccggcccaa gcaggcggag ttcgccgagg cgatccggga gagcgccgac
     4741 tcgctcctcg ccatcatcaa cgatgtcctc gacctgtcga agatcgaggc cgggaagatg
     4801 cgcgttgaca agaccgattt cgacttgagc gacatcgtcg atgcgtcggt taatatgttc
     4861 ctggagaggg tccgcgccaa ggggctgtcg ctcggctgta tgatcgacga cggggtgccg
     4921 acgctgctgc gcggcgaccc cggccggctg cggcaggtct tagtcaacct ccttagcaac
     4981 gccgtcaagt tcaccgagtc gggcgcggtc acactggaag tgacgggcgg ccccgaggcg
     5041 gacggccacg tgacgctccg cttcgaggtg aaggacacgg gcatcggaat cagccgcgag
     5101 gcgagacagc gcatcttcct ccccttcacg caggccgacg gctccgacac ccgcccctac
     5161 ggaggcaccg gtctggggct cgccatctca aggcagctcg tcgagttgat ggggggtgag
     5221 atcggtgtcg agagcgaggc cggccgcggc tccacgttct ggttcacagt cagatgcgcg
     5281 cagcaaccgg gcgccgcgcc ggacgcccca cgctccgatc tcgaggggct gagcctcctc
     5341 gtcgtggggt ccgacgccgc ccaccgcggg gttctcttac agcaggccgg acagtggaag
     5401 atgccggccg ccgaggccga ggatgccggg cgcgccctcg gcctgctgcg cgaggcgcag
//
LOCUS       DASVES010000525        18990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_619907, whole genome shotgun sequence.
ACCESSION   DASVES010000525 DASVES010000000
VERSION     DASVES010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18990
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619907"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VF240_22380"
     CDS             <1..1360
                     /locus_tag="VF240_22380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178022.1"
                     /translation="GRMVRTPLARRIPGSDRLNSVDCLLPKFDKATVEAVVKALREGS
                     GDTPPTGRVLINPKEMKPNPAVPDTVWEKFVSLPSQTRPQRGAKPAIRLTALAHELAS
                     DGLLPGAGGKAHAALHKVLDDFIAAREQAFAEKRAAVMTVEGRTIIADLKTGETKDGQ
                     FQADADDAVVGDAYRRTARILSPDIARTYTMERAKRKPEAEDDLDDALIEAREDVAAL
                     HLLENLQIQFDAEAKKLSDAWFVQYRKQIKELPDDRQEAYRQIVALSAEPQDVDLARP
                     VSRFEPTKAREADGSETDIPTYKQHLLCDEEGFYPAELRDWERQVLSAELKRNGFRFW
                     YRNPNRPSQDSLGIAYIEGDDTKVIRPDFIFFAEDDGEIVADIVDPHGFYLADALPKL
                     KGLARHAETHGHIYRRIEAVAKAGNKLRMLDLTRADVRQAVMGAASAEAVYRSAVAGD
                     YT"
     gene            complement(1529..1840)
                     /locus_tag="VF240_22385"
     CDS             complement(1529..1840)
                     /locus_tag="VF240_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178023.1"
                     /translation="MIQIMGRKQFRRAEQRARQERMMVRPVAECEYTVANQSKGTSYR
                     VRFLSINGKPFGNCDCLAGRPERGDAVPVPCKHLFAAAVVHVALARMDSRAMGRSTRI
                     R"
     gene            complement(1873..2148)
                     /locus_tag="VF240_22390"
     CDS             complement(1873..2148)
                     /locus_tag="VF240_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178024.1"
                     /translation="MMTNNNDANLNLVQTELRFILTQATTGGVESAQLIQMAAESALQ
                     ALFEHSLSLEPASDPDAEFDYPAPPEGGPVLVGSATFGRERVGAFVI"
     gene            2236..2484
                     /locus_tag="VF240_22395"
     CDS             2236..2484
                     /locus_tag="VF240_22395"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7178025.1"
                     /translation="MEKRLYTTSEAAALLDVTPARVRQMVARGEIAFERIGRDNFVTA
                     EAITEAQKRKTKPGPTPKPKAEGATKPRARKSSRTVRS"
     gene            complement(2643..2716)
                     /locus_tag="VF240_22400"
     tRNA            complement(2643..2716)
                     /locus_tag="VF240_22400"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2680..2682),aa:Pro,seq:tgg)
     gene            complement(2785..3396)
                     /gene="rdgB"
                     /locus_tag="VF240_22405"
     CDS             complement(2785..3396)
                     /gene="rdgB"
                     /locus_tag="VF240_22405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX7178026.1"
                     /translation="MPEELLIATRNAGKVLEFSRLLGDMSVIMRGLSDFPDVGEVEES
                     GETFEENATIKARVYAGLTGLSTLADDSGLQVDALGGAPGVRSARYAGPRATDAERNT
                     LLLSELHAAHDAGRRARFVCVLALYRPADDSLKLFGGTCEGRIGHEPRGDGGFGYDPI
                     FIPDGYSRSFGELPSDVKDRISHRAVALRAVRSFLAELSGCTT"
     gene            3695..4930
                     /locus_tag="VF240_22410"
     CDS             3695..4930
                     /locus_tag="VF240_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178027.1"
                     /translation="MRALGSYLRISNHPFSETERTSLPKRSFTAETRIARDLMLRCTE
                     TLKSSSFAFSFSPLPADAGQQEGEPSLPRESVPNPDGDRFAYAASILEDLYALYDCVV
                     ETENVSFESWLGLAGLGARQLALTGYEHLVEETSERRGRSSVSPELCALASQISPDSL
                     SADVSAILTQLYGMLDLLGFVESSLAADEPLKGTLPVFTLLREESRALLDLIVERTLR
                     IEGLDPAVHEYLDGVAYAIRMELRKAFEHELVGLSSQRSAPLVFGKVENAHGLLRDCF
                     QQSVIGVARAFDAQLDGSGLFNNFRTKLEQSLLLRQELWEVLAAVRRADQEQEPAALR
                     NLMLRLSHFREGSLRFLMYKDWEALERFVAEATAAKSSADLQPVLHRFHAFLETLFGQ
                     VNMRAVLADHPFDPDTSQD"
     gene            complement(4988..6727)
                     /locus_tag="VF240_22415"
     CDS             complement(4988..6727)
                     /locus_tag="VF240_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178028.1"
                     /translation="MWKIILVASAVLFLAVLWLWWNRPEQVDMAEYVPADALVYLESN
                     SIPEVVKGIVATQAWKEVAVAAGVGSGADQYGWLSHVAEWTGLGSHETVIVARAQVAV
                     CILGFSAGEYPDATLKISPRGAVVIETHTSGARVRAAVENAVGRFARRSLGASEPARK
                     EEGGAFFYTWAAPEDGRRRVVAAVLGTVAVIGNDEATVQACLSVQRGERPSLAGDPRL
                     RELRSRLGGEGTLAFGYAPSGSAAKVVEAAAPLFVGQASDEPRVQSLLASHLPKLAHR
                     VVGAAAWSTHVEGGALIDRYLLLLPDGLARRLSNALPSTESQSAAAAELLPADMHQVS
                     YYTYRDPEAAWLALNASLSTQVDSLQAPMVTLALESMLQPYGVESPREFLRATRSEVI
                     TARLDETSHSKVLIVGIRDKTELLEQVRKHLGAGAQATTVGADEMWVSTEPERGAAVF
                     LGDYLMMGQEEDVRRCISARAERRSLAGSERFKSPQDTAGPAPHVLTFASDESDARTF
                     VSVFRRRRQTESIDAEKLNQTLRSLPYSVSETRVTEEGVEKRTRSAFGLFGSIVARFA
                     PPADTSDTQAGAP"
     gene            7346..8407
                     /gene="pilM"
                     /locus_tag="VF240_22420"
     CDS             7346..8407
                     /gene="pilM"
                     /locus_tag="VF240_22420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX7178029.1"
                     /translation="MFKKSLGKKNLVGLDIGSSSVKAVELQGKMGSLQLASLGYETLQ
                     PDTVVDGQIMELNDVSNAIAGLFSAHQIKTDRVAAGVSGSSVIVKNIIVPQMSEGELE
                     ESIDWHAEEHIPFDIADVSLDYQIVGSGADSLHVLMAACKRDFIANIKQAIQLAGKQP
                     AIIDVDAFALQNCYEVNYEPRADQTVALLNVGAATMNINILKGVQSVFTRDVSVGGNQ
                     YTSLLQKELGLTFEQAEAVKRTGKAPEGTDSGELEAILETVSDMLALEISKTFDFYRA
                     TADDSEAAVQKILISGGGCKLRGLTEYLESRFELPVELFDPFRNIKIDARKFDPDFMR
                     DLVPEMAVAVGLALRGVEA"
     gene            8408..9097
                     /locus_tag="VF240_22425"
     CDS             8408..9097
                     /locus_tag="VF240_22425"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX7178030.1"
                     /translation="MIKVNLLDSVTDRTHSVAAVEARVANPRARSWILMSVVFGLVVL
                     GMGVDYVSANYKNTSAKDELARQEEIAAKMQEINKQQADLEKKINEVKSRIEAIKKLR
                     ASQQGPVSLLSELNSRMPQLKDFSLTEVEQKNGELTIQGHSSSEDAVAQFARALEFSD
                     KVFTNVSVETERKIVNPEDTDWTPADGEIDDEAPKPEVVKFKITCRYGEQPAEPAAAP
                     DAKKKKDAPAA"
     gene            9164..9814
                     /gene="pilO"
                     /locus_tag="VF240_22430"
     CDS             9164..9814
                     /gene="pilO"
                     /locus_tag="VF240_22430"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7178031.1"
                     /translation="MKKLEALQEKPWYMQLAAFGVVGILLYACFWYFVTKGTRDETTE
                     IEVKIGELQMENAKAQAAEQRLNDFRASYARVQADYDDLKALLPEHKELTMVLSSIHD
                     RARGQLAVTKFKPKADEQQDFYSSKLIEVGVSGSYNKLGQFFSQMASYQRIVSITDFK
                     LTGLDKKKNEDKDMMQKGRTLNAEFKLKAYYASPDRLQNVAPVAATAGVKPPVATK"
     gene            9859..10518
                     /locus_tag="VF240_22435"
     CDS             9859..10518
                     /locus_tag="VF240_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178032.1"
                     /translation="MMTMRSKKRIFAAACGLLVAAWCAPLAGAQEAANNTNYRTAKRD
                     PFTNYKVMFRRKVAKKESPKPMPITAPSIQARIEQFKAQKRAAINLQQPAPKPTTALL
                     LGEVRVTGIFRTPRGVAAMVEATPIKLSYVIYPGETFYNGMLVAIEDSRLVFRKETRW
                     TDGRRDVAVETKPLEQPSAADALTAQKTGEAGQPAAPGKSQPEAPARKDDAKALASKS
                     N"
     gene            10814..13015
                     /gene="pilQ"
                     /locus_tag="VF240_22440"
     CDS             10814..13015
                     /gene="pilQ"
                     /locus_tag="VF240_22440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX7178033.1"
                     /translation="MSHPEKIIKAVAARVALVLSLALPLTTPALASAATATTPPAVKS
                     VRASVSGAATFITVDATSPMAYSVRRPGDDLLVVELHGVNASALAPSYTVASPLVAGV
                     AVKKSMHDGGAQTSLEISLRAPARERSHMSDTSLIVELWPEKTAATADAARPVTRTDA
                     AAKTNDPVPGPADSAKAQQPADRTTGGEPASQQGQRYGQPGFVGEPISLNVVNADIRD
                     ILNYITEQYGINFIIDASVLAIPVTVNVTDVPWNIALDSILKANRLGVEANGNILRVA
                     TTGVLAGESEVQSRIRAAALENSPLTTEYVRLNYARASGTLSNAAGTTGNFSGGSTGQ
                     SFSGGTGGQTDPGILPIIKRRLSKRGAVEYDGRSNTLIITDVQENIDSIRKLVTLLDQ
                     PEPQVEIETRIVIATRNFSRDLGVQLQAAVFGARGAGGSFETAPAQTGLIPGGLPGGL
                     VNTPPSSGGLGVPGASTVIGLTTGIFGTAQIGALITAAERNGQAKIVATPRVTALNNR
                     PAQIESGSQIPVVTPQLATGTGGTLGGSVFTTTFVSVPLRLSVTPQITDAGTVILRVV
                     AENNSVNLTLVNSFGTPGIDTQRMQTEVLVPDGGTTVVGGVLNDREAETQNRTPGLSG
                     IPVLGNLFKRRTTRRDTDELLFFITPRIYRPDYQGRRVESSTQTGTRFVSIPQPVPLG
                     NPETNTPTQQDQLQQQNQQPAPIIIPMPANPQQGQSQTTTPPSQTGETPRP"
     gene            13032..13397
                     /locus_tag="VF240_22445"
     CDS             13032..13397
                     /locus_tag="VF240_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX7178034.1"
                     /translation="MFREALESINGRTEGALGSLIMGTDGIAVEKLLLEDGIEANIDV
                     AAAEVTSLVRSAQRAGRDTGLGAVRELILTFEGMNIIARLITADYFVVLALQPDGNLG
                     RGRFELRKAEMDLAREFAM"
     gene            complement(13555..14337)
                     /locus_tag="VF240_22450"
     CDS             complement(13555..14337)
                     /locus_tag="VF240_22450"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEX7178035.1"
                     /translation="MWRLIKTFALFLFALFTLWIVYEAVTFPRVSRLRTQNPETTSMI
                     ETRLEEARKEGREPRRVQQWVPLEKISPELQRAVLAGEDTNFAAHDGFDYEAIRKAWE
                     EGRRAAEKEAKEEGETDEQSWIPDLSHFKRGASTITQQLAKNLYLSSERSFARKGREV
                     FITYFLERSLPKRRILEIYLNVIEWGDGVYGAEAAAQTYFNKPAATLNAREAAFLSAM
                     IPNPRTVFNPVPTAQNPKSNLKRVQRRQRAILRGMPHVKLPG"
     gene            complement(14457..15410)
                     /locus_tag="VF240_22455"
     CDS             complement(14457..15410)
                     /locus_tag="VF240_22455"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7178036.1"
                     /translation="MKVFYTPRYYADIGEGHVFPIRKFEMVRDRLLREGTLAPQEIVE
                     PRPAAVEDVLLVHTEDYVTRLRAGALTPRELRRLGLPWSKALVRRSFLATGGTLGAAR
                     RALAERVGSNLAGGTHHAFADRGEGFCVLNDVAVAIRVLRRDGLIRRAAVVDCDVHQG
                     NGTATIFAGDADVYTFSMHGAKNYPLFKAASTLDVELPDATADDEYLDALARHLPRVF
                     EHAPDIVFYLGGADPYAGDKLGRLALTISGLRARDELVLRACRDAGLPVATVMSGGYA
                     ADIDDTVEIHCNTIRAAKAVFEGVQGSKLKAQSAGPDSTAI"
     gene            16674..17867
                     /locus_tag="VF240_22460"
     CDS             16674..17867
                     /locus_tag="VF240_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178037.1"
                     /translation="MGNCAIGDPTPTPTPTPGPAGGDCDPAARPTCPYSDCNLRTGQW
                     ECCDQNTRPNSNCPCEYSNLSGSVEWECHLNCLASGYSPANFQHYRATGCAPNSYNEG
                     NCCKCYNQNPTCPSGFTWDKSQCKCCNSSGQCTETVAGGGGSGCDNPPLPDGTCADGS
                     YVDATGCCGGDWTCNPYCCQTGEGCNNFGDPEPPPLTDILNDSPSLAPNPNDDCCDYS
                     PILVDVSGDGFDLTGAHGGVRFDMRGDGRPAEMSWTRSGSDDSWLFLDRDGNGVVDNG
                     KELFGNYTEQPRSVRPNGFRALAQFDKAERGGNGDGWIDSRDGVFASLRLWRDANHNG
                     VSEPGELHTLPSFKVVSFSLDYKTSLQRDRHGNRYRYRAPVRIDDGRRLFGRWAWDVF
                     LVEAQ"
     gene            complement(17942..18772)
                     /locus_tag="VF240_22465"
     CDS             complement(17942..18772)
                     /locus_tag="VF240_22465"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7178038.1"
                     /translation="MRRLLSMLGRVRAFVVRDFQLAVSYRLEFFMRIMSILFVTTTLY
                     FISRIFTGRFDTPYDQWRDPFLAWITALPFLNYFMVGFSSLANAIRSEQSQGTLEGVL
                     MTPINVPTLIIASSAWDFVLATFHSFLYLFFGWVLFGVHYRGNFLLALLFLLLTTLVL
                     ASIGILSASFAMVFKRGDPFGVIIGTGSALFSGVFFPTQLLSSVAGKGFGLVSSLFPT
                     TYGIDGIRRVLIQGQGFAEVRDPLVTLLIFLAALLPFSLWVFSRAVRRAKREGSLIQY
                     "
     gene            complement(18769..>18990)
                     /locus_tag="VF240_22470"
     CDS             complement(18769..>18990)
                     /locus_tag="VF240_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178039.1"
                     /translation="VSDEGGGSVAVNFMRETSDDSLDRALKLLLADGARVVSCESERA
                     TLLDVIERYEKESDGPAATTAAGAAEVRG"
BASE COUNT         3704 a         6143 c         6069 g         3074 t
ORIGIN      
        1 gggccgcatg gtgcgaacgc cgctggcgcg gcgcatcccc ggcagcgaca ggctcaactc
       61 cgtggattgc ctgctgccga agttcgacaa ggcaacggtc gaggccgtcg tcaaggcgct
      121 gcgcgaaggc agcggcgaca cgccgccgac gggacgcgtt ttgattaacc cgaaggagat
      181 gaaacccaac ccggccgtac cggacacggt gtgggagaag ttcgtctcgc tgccttctca
      241 gacccggccg caacgtggcg cgaagccggc gatccgcctt acggcactgg cgcacgagct
      301 ggcgtcggac gggctgctgc ccggagcggg cggcaaggcg cacgcggcgc tgcataaggt
      361 actggacgat ttcatcgccg cgcgggagca ggcgtttgcc gagaaacgcg cggcggtgat
      421 gaccgtcgag ggccgcacca tcattgccga cctcaaaacc ggcgagacga aggacggcca
      481 gttccaagcc gacgcggatg acgccgtcgt cggcgacgcg taccgccgca cggcgcgcat
      541 cctcagcccc gacattgccc gcacttacac gatggaacgg gcgaagcgga aaccggaggc
      601 ggaggacgat cttgacgacg cgctgatcga ggcccgcgag gacgtggccg cgctgcacct
      661 cttggagaac ctgcaaatcc agttcgacgc cgaggcgaaa aaactttcgg acgcgtggtt
      721 tgtccagtac cggaagcaga tcaaggaact accagatgac cggcaagagg cgtaccggca
      781 gatcgtcgcg ctcagcgccg agccgcagga cgtggattta gcccggcccg tgtcgcgctt
      841 cgagccgacg aaggcacgcg aggcggatgg ttcggaaacg gatattccga cctacaagca
      901 gcatctgttg tgcgacgaag agggcttcta tcccgccgag ttgcgcgatt gggaaaggca
      961 ggttctgtcg gcagagctga agcgaaacgg ctttcggttc tggtatcgca atcccaaccg
     1021 cccgagccaa gattcgctag gtatagcata catcgagggc gacgacacga aggtcatcag
     1081 gccggacttc atcttcttcg cggaagacga tggcgagatc gtggcggaca tcgttgatcc
     1141 gcacggcttc tacttggccg atgcgttgcc gaaactgaaa ggcttagcac gccacgccga
     1201 aacgcatggg cacatatacc gccgcattga agccgttgcg aaagcgggga acaaactacg
     1261 catgttggat ttgacacgcg ccgatgtccg ccaagcggtg atgggagcag cgagcgcgga
     1321 agcggtttac agaagcgcgg tggccggtga ctacacgtga cctgcttctg ccggaggcgc
     1381 ggctggaaaa cctcagtgac gaatttttag aacgcgtgtc ggccctgata tgtagcaaga
     1441 tggtttggct cggtggcggg aggcctgaca acggcctgca cccgaccgcg ggcgtgagca
     1501 gggggggcgg ctgacgcgcc cgtccccttt accggatgcg cgtcgagcgg cccatcgccc
     1561 gcgagtccat ccgggcgagc gcgacgtgta cgaccgcagc ggcgaataag tgcttgcacg
     1621 gcaccggcac ggcatcgccg cgctcggggc ggcccgcgag acagtcgcaa ttcccgaacg
     1681 gctttccgtt gatcgagagg aagcggacgc gatagcttgt gcccttcgat tgattcgcca
     1741 ccgtgtactc gcactcggcg acggggcgaa ccatcatgcg ctcttgccgc gctctctgct
     1801 ctgcccgtcg aaactgtttg cggcccatta tctgaatcac ggttttatct cctcgcaggg
     1861 tttggggtct ggtcagatga cgaaggcgcc gacgcgctcg cggccgaagg ttgcggagcc
     1921 gactagcacc gggccgccct cgggcggggc cgggtagtca aattccgcgt cggggtcgct
     1981 cgcgggttca agcgaaaggc tgtgctcgaa aagggcttgg agcgcgctct cggcggccat
     2041 ctggataagc tgcgcgcttt cgacgccgcc cgtagtggcc tgcgtgagaa tgaatctgag
     2101 ttccgtctgg actaggttaa ggttggcgtc gttgttgttc gtcatcatgt aaagatatta
     2161 actcatgggt aactatctgt caagctaata attcactatt gcgataataa ctcacctgtg
     2221 ttttaatgtg gcgctatgga aaagaggctc tacaccacaa gtgaggccgc ggcgctctta
     2281 gacgtgacgc cggcccgcgt gcggcagatg gtcgcgcggg gcgagatcgc tttcgagagg
     2341 atagggcgcg acaacttcgt gacggccgag gcaatcactg aggctcagaa gcggaagacg
     2401 aagcccggcc cgaccccgaa gccgaaggcc gagggggcaa cgaagccacg ggcacgcaag
     2461 tcttcccgga ctgtgcgctc ctagtgtcta cgtagaaggg cacgcaagcc gcgcgggtag
     2521 gctaagaagt aaggcgcgtg gggtaagtgc ccgaaggtgt ttgtgagtgg ttttgtgtgt
     2581 gaatgtgtgt gtaaaagaaa aacggccacc gcacgatggc cgtaaatcct tgaaactaaa
     2641 aatcggggcg gcaggattcg aacctgcgac ctctcggtcc caaaccgagc gcactaccag
     2701 gctgtgctac gccccgccgt catcgaccga ataaaaactc ggaacacaaa cgcggttcat
     2761 tctatcggcc gtatctccaa agggtcaagt tgtacatccc gatagttccg cgaggaatga
     2821 tcgcacggcg cggagggcga cggcccggtg gctgatgcgg tctttaacat cagacggcag
     2881 ttcgccgaag cttcgactgt agccgtccgg gatgaaaatg ggatcgtagc cgaagccgcc
     2941 atctccccgc ggctcatgcc cgatgcggcc ctcacaagtc ccgccgaaca atttgagaga
     3001 atcgtccgcc ggccggtaga gggcgagcac gcagacgaaa cgcgcgcggc gtccggcgtc
     3061 atgcgcggcg tgcaattcgg agagtaagag tgtgttgcgt tcggcgtcgg tcgcgcgcgg
     3121 gcctgcgtaa cgggcggagc ggacgccggg ggcgccgccc agcgcatcga cctgaaggcc
     3181 ggagtcgtcg gcgagagtag acaggccggt caggccggcg taaacgcggg ctttaattgt
     3241 cgcattctcc tcaaacgttt cgccggattc ctcaacctca cccacgtcag ggaaatcgga
     3301 caaacccctc ataattactg acatatcgcc cagaagtcgt gaaaattcga gcaccttgcc
     3361 ggcgttgcgc gtggcgatga gtaattcttc cggcatcgag tgattcttac cgcgtcggtt
     3421 ctggccggcg ccagcgcggg cgtaaccgcg agtcgaaaaa agattcttta cccgcccgcg
     3481 ggtttcgtgc taacaatcat aaaaggcgcg gcagactccc ctgctgtcgc gaaggatttc
     3541 aaagagagaa aaatatttgg taacgagcgc agagattata gagcagagtg tgcccgaagg
     3601 ggtacgcgga gacctgtcgg cattcacgaa gcaggtcgag agggaagttg aatcggccgg
     3661 gccgaccggg cttccgtggg aattatgcgt gcatttgcgc gcccttggaa gttatctgag
     3721 gatttccaat caccccttct cggagaccga gcgcacgagc cttccgaagc gcagctttac
     3781 cgctgagacg cgcatcgccc gcgacctgat gcttcgctgc acggagacgt tgaagtcttc
     3841 ctcctttgcc ttctccttct cgcccctccc ggctgacgcg gggcaacagg agggcgagcc
     3901 gagtctgccg cgcgagtcgg tcccaaatcc agacggcgac cgcttcgcct acgcggcctc
     3961 aatcctggaa gacctctacg cgctctacga ctgcgtggtg gaaacagaaa acgtcagctt
     4021 cgagtcgtgg ctgggtctcg cggggctggg ggcgcgtcag ctcgcgttga cgggatacga
     4081 gcacctcgtt gaggagacct cggagcggcg ggggcggtcg tcagtatcgc cggagttgtg
     4141 cgcgctcgcg tcgcaaatct cgcccgactc gctctcggcc gacgtgagcg cgatcctcac
     4201 gcaactttac gggatgctcg atctgctggg attcgtagag tcgtcgctgg cggcggacga
     4261 gccgctaaag ggaacgctcc ctgtcttcac gttgttgcgc gaggaatcgc gcgccctgct
     4321 cgacctcatc gtcgagcgca ccctgcggat agagggactc gacccggccg ttcacgagta
     4381 tctcgacggg gtcgcctacg ccatcaggat ggaactgagg aaggcttttg aacacgagtt
     4441 ggtggggctg tcatcacagc gttccgcgcc gcttgtcttc ggcaaggtgg agaacgcgca
     4501 cgggcttctg cgcgactgct tccagcagtc cgtcatcggc gttgcgcggg cgttcgacgc
     4561 gcagctcgac gggtcgggcc tcttcaacaa tttccgcacg aagctcgaac agtcgctcct
     4621 gctgaggcag gagttgtggg aagtgctcgc ggccgtgcgg cgcgcggatc aagagcagga
     4681 gccggccgcg ctgcgaaacc tgatgctgcg gctgtcacac ttccgcgagg gcagcctgcg
     4741 cttcctgatg tacaaggact gggaagctct ggagaggttc gtcgcggagg cgaccgcggc
     4801 gaaatcctcg gccgaccttc agcccgtgct ccaccgcttc cacgcctttc tcgaaaccct
     4861 tttcggacag gtcaacatgc gcgccgtcct cgccgatcac cctttcgacc ccgacacatc
     4921 gcaggactga aaccccgccg ctgtcccgaa ccgacacgcg ccgggacgcg cgcaaccgtc
     4981 tacacattca gggcgcgccc gcctgcgtgt ctgaagtgtc ggccggcggc gcaaaccggg
     5041 cgacgattga gccaaacagc ccgaacgcgg agcgcgtgcg cttctcgaca ccttcctccg
     5101 tcaccctcgt ctcgctcaca ctgtaaggaa gactgcggag cgtttggttc aacttctccg
     5161 cgtcaatgct ctctgtttgg cgacgccgcc gaaacacact tacgaacgtg cgggcgtcgg
     5221 attcatcaga agcgaaagtg aggacgtgag gggctggccc ggccgtgtcc tgcggcgact
     5281 tgaagcgttc cgagccggcc agcgaccggc gctccgcgcg cgccgagata cagcggcgca
     5341 cgtcttcctc ctgtcccatc atcaggtagt cgccaagaaa aaccgcggcc ccgcgctcag
     5401 gttccgtgga gacccacatc tcgtcagcgc cgacggtcgt cgcctgcgcc cctgcgccca
     5461 gatgtttacg cacctgttcg agtagttcgg tcttgtccct gatgccgact atcaagacct
     5521 tgctgtggct cgtctcgtcc agacgcgccg tgatgacttc cgacctcgtc gcgcgtaaga
     5581 actcccgcgg cgactcgacc ccgtagggct gcaacatgga ttcgagggcg agcgtcacca
     5641 tcggggcctg caaggaatcg acttgagtcg agaggctcgc gttcaaggcg agccatgccg
     5701 cctcggggtc gcggtaagtg taataggaaa cctgatgcat gtcggcggga agaagctcgg
     5761 cggctgcggc agattggctc tcggtggagg ggagtgcgtt cgagaggcgg cgggcgaggc
     5821 cgtcgggtag gagcaacagg tatcggtcga taagagcgcc gccctcgacg tgggtgctcc
     5881 acgcggccgc gccgacaacc ctgtgcgcga gcttcggcaa gtggctcgcg agcagactct
     5941 gcacgcgcgg ctcgtcggaa gcctgaccga cgaagagcgg cgccgccgct tcgacgactt
     6001 tcgccgcgct gcccgacggg gcatagccga aggcgagcgt gccctcgccg ccgagacggg
     6061 agcgcagctc gcgcaaacgc gggtcgccgg ccagagacgg ccgctcgccc ctctgcacgg
     6121 acaggcacgc ctgaactgtc gcctcgtcgt tcccgatcac cgccacggtt cccaggaccg
     6181 cggccaccac acgcctgcgc ccgtcctccg gcgcggccca cgtgtagaag aatgccccgc
     6241 cttcctcctt tcgcgccggc tccgaggcgc cgagcgaacg tcgtgcgaac ctgccgaccg
     6301 cattctccac cgcggcccgc acccgcgcgc cgctcgtgtg cgtctcgatg accacggcgc
     6361 cacgcggcga aatcttcagc gtcgcgtccg ggtactcacc ggcgctaaac cccagaatgc
     6421 aaaccgccac ctgcgcgcgc gcgacgatga ccgtctcgtg tgagccgagg cccgtccatt
     6481 ctgccacgtg tgacagccag ccgtactggt cggccccgga gccgacgccg gccgccactg
     6541 ctacctcctt ccacgcctgc gtcgcgacga tgcccttcac aacttcgggg atgctgttgg
     6601 attcgagata gaccagcgcg tcggccggaa cgtactccgc catgtctacc tgctccggac
     6661 ggttccacca gagccagaga acggcgagga agagaaccgc cgaggcgacg agaatgattt
     6721 tccacacgcg cgacatgtgc ctcacatcct agccgcgctc gtaatcgcga ggcaagataa
     6781 tctcagtcaa ttcacctgca tcacggcccg cgtcacagag tgtagataag cttctgcggc
     6841 ctgtaggaaa cgctctgcgc cgacccttca attgtggaag acgaaggtcg caaaccccga
     6901 aacaatttca ggaagtggca aggagctaat gccaaaccta tgtgcgtcag tagttaaaca
     6961 tggcaaaatt gtatttgaca ctattcaacc acgtagttta tagtcttctt ggcaggcgga
     7021 ttgtcctgtt tcgtcttagt catatctcgc agcaggtcgg ggccggtcga gtctacagcc
     7081 gcgggccgga gcggcaacaa tccaaagcgc gccgcccgac tccacattgg cactgaaccg
     7141 acaagagcac acctcacagc cgactcggac gcgcccacaa ctctccttca agccgtcagt
     7201 cccgcaacga aatgagaccc gcggaagtat ccacgagcag gacgcgcgcc gcgcgcggct
     7261 gccattcccg cgcccgccgc gcccgcgccc gcctccgccg tctccaccgc cgcagaatgt
     7321 tgatcgccag gaacagataa agattatgtt caagaaaagc ctcggcaaga aaaacctcgt
     7381 gggcctcgac atcgggtcga gctccgtgaa ggccgtcgaa ttgcagggca agatgggcag
     7441 ccttcaactc gccagcctcg gctacgagac gctccagccc gacaccgtcg ttgacggcca
     7501 gatcatggag ctgaacgacg tgtcgaacgc catcgccggc ctgttcagcg cccaccagat
     7561 caagaccgac cgcgtggccg cgggagtgtc gggcagctcc gtcatcgtca agaacatcat
     7621 cgtcccccag atgagcgagg gcgaactcga agagtcaatc gactggcacg ccgaggagca
     7681 catcccgttc gacatcgcgg acgtgagcct cgactaccag atcgtcggct ccggcgccga
     7741 ctcgctgcac gtgctgatgg ccgcctgcaa gcgcgacttc atcgccaaca tcaagcaggc
     7801 catccagctc gccggcaagc agccggccat catcgacgtt gacgccttcg ccctccagaa
     7861 ctgctacgag gtcaactacg agccgcgcgc cgaccagacc gtggcgcttt tgaacgtcgg
     7921 cgcggcgacg atgaacatca acatcctcaa aggcgtgcag agtgtgttca cgcgcgacgt
     7981 gtcggtgggc ggcaaccagt acacgtcgct gcttcagaaa gagctggggc tgacgttcga
     8041 gcaggccgag gccgtgaagc gcacgggcaa ggctcccgag ggcaccgact ccggggagtt
     8101 ggaggccatc ctggagacgg tctccgacat gctggcgctc gaaatctcga agacgttcga
     8161 cttctaccgc gccacggccg acgacagcga ggccgccgtc cagaagattc tcatctcggg
     8221 cgggggctgc aagctgcgcg ggctgaccga atacctggag agccgcttcg agcttccggt
     8281 cgagttgttc gaccccttcc gcaatatcaa aatcgacgcg cgcaagttcg accccgactt
     8341 catgcgcgac ctcgtgcccg agatggccgt cgccgtcggc ctcgccctga gaggagtgga
     8401 agcttaaatg attaaggtca acctactgga ctcggtcacc gaccggacgc acagcgtcgc
     8461 cgccgtcgag gcgagggtcg ccaacccgcg cgcgcgcagt tggattctga tgagcgtcgt
     8521 cttcggcctc gtcgttctgg ggatgggcgt cgattacgtg agcgccaact acaagaacac
     8581 gagcgcgaag gacgagctgg cgcggcagga agagatcgcc gcgaagatgc aggagatcaa
     8641 caagcagcag gccgacctcg agaagaagat caacgaggtc aagtcgcgca tcgaggccat
     8701 caagaagctg cgcgcgtcgc agcaggggcc ggtgtcgctc ctctccgagt tgaactcgcg
     8761 gatgccgcag ctcaaggact tctccctgac cgaggtcgag cagaagaacg gcgagctgac
     8821 cattcagggc cactcgtcca gcgaggacgc agtggcgcag ttcgcgcgcg cgctcgaatt
     8881 ctccgacaag gtcttcacca acgtcagcgt cgagaccgag cgcaagatcg tcaatcccga
     8941 ggacaccgac tggacgccgg ccgacggcga gatcgatgac gaggccccca agcccgaggt
     9001 cgtgaagttc aagatcacgt gtcgctacgg ggagcagccg gcggagcccg cggcggcccc
     9061 agacgcgaag aagaagaagg acgcgccggc cgcctgacag gtggcgcgga agtagacgga
     9121 agcggccgcg cccggccgct cgaccgaagg aacccaaaag accatgaaga aactcgaagc
     9181 cctccaagag aaaccctggt acatgcagct cgccgcgttc ggcgtcgtcg gcatcctgct
     9241 ctacgcctgc ttctggtact tcgtcaccaa gggcacgcgc gacgagacga cggagatcga
     9301 ggtgaagatc ggggagttgc agatggagaa cgcgaaggcg caggccgccg agcagcgcct
     9361 gaacgacttc cgcgcctcct acgcgcgcgt ccaggccgac tacgacgacc tgaaggcgct
     9421 gctgcccgag cacaaggagc tgacgatggt gctctccagc atccacgacc gcgcgcgggg
     9481 ccagctcgcc gtgacgaagt tcaagcccaa ggccgacgag cagcaggact tctactccag
     9541 caagctgatc gaggtcggcg tctcgggcag ctacaacaag ctcggacagt tcttctcgca
     9601 gatggcctcc taccagcgca tcgtctcgat tacggacttc aagctgacgg gactcgacaa
     9661 gaagaagaac gaagacaagg acatgatgca gaaggggcgg acgctcaacg ccgagttcaa
     9721 gctcaaggct tattacgcct cgcccgaccg gctccagaac gtcgcgccgg tcgcggcgac
     9781 ggctggcgtc aagccgccgg tcgcgacgaa gtagccgcag ttcgcggcgt cgcataaaac
     9841 ctcggagtta aggaagagat gatgacgatg agatcgaaaa agagaatctt cgccgccgcg
     9901 tgcggactgc tggttgccgc ctggtgtgcg cccctcgcgg gcgcgcagga ggccgccaac
     9961 aacaccaact accgcacggc gaagcgcgac ccgttcacga actacaaggt catgttccgg
    10021 cgcaaggtcg cgaagaagga gtcgcccaag ccgatgccca tcacggcgcc ctccattcag
    10081 gcgcgcatcg aacagttcaa ggcgcagaag cgcgcggcga tcaacctcca gcagcccgcg
    10141 ccgaagccga cgacggcgct cctgctcggc gaggtgcgcg tcaccggcat cttccgcacc
    10201 ccgcgcggcg tggcggcgat ggttgaggcc acgccgatca agctctccta cgtcatctat
    10261 cccggcgaga ctttttacaa cgggatgctc gtcgccatcg aagactcgcg gctcgtcttc
    10321 cgcaaggaga cgcgctggac cgacggacgg cgcgatgtcg ccgtcgagac caagccgttg
    10381 gagcagccca gcgcggccga cgcgctgacg gcgcagaaga cgggcgaggc gggacagccg
    10441 gccgcgcccg gcaagagcca gcccgaagcc cccgccagaa aagacgacgc caaggcgctc
    10501 gcctcgaagt ctaactaaag cttcggcccg cgcgcaggcc gcctgcccct ctcccagggc
    10561 agcggcggcg cgcgggccgc tctcccgtga gccgtcaacg gctcgcacgc tcggcgcggg
    10621 acaagacgat tgcccgcgcc ccccacctcg acaccgtttg acaggcaggc caggcggaca
    10681 ccgcgttgtg cggcgcgcgc ttaatgagga caacacgccg caggcgtttg cggtcccaac
    10741 gagcgttagc gcgtggcggt ttcgtgatgc ccggccagct cacccgaaga taagtcccac
    10801 gaaggataaa ggtatgagcc acccagaaaa gatcatcaag gcggtcgccg cgagagtcgc
    10861 gctcgttctc tcgctggcgc tgccgctcac cacccccgcc ctcgcctccg cggcgacggc
    10921 gacgacaccg cccgcggtga agtctgtgcg cgcatccgtc tccggggcgg cgaccttcat
    10981 caccgtggac gcgacctcgc cgatggccta cagcgtccgc cgccccggcg acgacctcct
    11041 cgtcgtcgag ctgcacggcg tcaacgcctc ggccctcgcg ccgagctaca cggtcgcttc
    11101 gccgctcgtc gcgggcgtcg cggtcaaaaa gtcgatgcac gacggcggcg cgcagacgag
    11161 tctcgaaatc tccctgcgcg cgcccgcgcg cgagcgctcg cacatgtccg acacgtcgct
    11221 catcgtcgag ctgtggcccg agaagaccgc ggccacggcg gacgccgcgc ggcccgtgac
    11281 ccggactgac gcggccgcga agacgaacga ccccgtgccg gggccggcag actccgccaa
    11341 agcgcagcag cccgccgacc gcaccacggg cggcgagccg gcttcgcagc aggggcagcg
    11401 ttacgggcag cccggattcg tcggcgagcc gatcagcctt aacgtcgtca acgccgacat
    11461 ccgcgacatc ctcaactaca tcaccgagca gtacggcatt aacttcatca tcgacgcctc
    11521 ggtgctggca atccccgtca cggtcaacgt cacggacgtg ccgtggaaca tcgcgctcga
    11581 ctcgatcctg aaggccaacc gcctcggcgt cgaggccaac ggcaacatcc tgcgcgtcgc
    11641 cacgaccggg gtgctggccg gcgagtccga agtgcagtcg cgcatccgcg ccgcggcgct
    11701 ggagaactcg ccgctcacga ccgagtacgt ccgcctcaat tacgcgcggg cttcgggcac
    11761 gctctcgaac gccgccggca ccaccggcaa cttctccggc ggctcgacgg gccagagctt
    11821 ctcgggcggc acgggcgggc agaccgaccc cggcatcctg ccgatcatca agcgccgcct
    11881 ctccaagcgc ggcgcggtcg agtacgacgg ccgcagcaac acgctcatca tcaccgacgt
    11941 tcaggagaac atcgactcga ttcgcaagct cgtcacgctc ctcgaccagc ccgagccgca
    12001 ggtcgagatc gagacgcgca tcgtcatcgc cacccgcaac ttcagccgcg acctcggcgt
    12061 ccagcttcag gccgccgtct tcggcgcgcg cggcgcgggc ggctccttcg agaccgcgcc
    12121 cgcacaaacc ggcctgatcc ccggcggcct gccgggcggc ctcgtcaaca cgccgccctc
    12181 ctccgggggc ctcggcgtcc ccggcgcgag caccgtcatc ggtctgacga cgggcatctt
    12241 cggcacggcg cagatcggcg cgctcatcac ggcggccgag cgcaacggtc aggccaagat
    12301 cgtcgccacc ccgcgcgtca ccgcgcttaa caaccgcccg gcgcagatcg aatcggggtc
    12361 gcagattccg gtcgtcacgc cgcagctcgc caccggcacc ggcggcacac tgggcggcag
    12421 cgtcttcact accactttcg tctccgttcc cctgcgtctg tccgtcaccc cacagatcac
    12481 ggacgccggc acggtcatcc tccgcgtcgt cgccgagaac aacagcgtca acctgacgct
    12541 cgtcaactcc ttcggcacgc cgggcatcga cacgcagcgc atgcagacgg aagtgctcgt
    12601 gcccgacggc ggcacgacgg tcgtcggcgg ggtgctcaac gaccgcgagg ccgagacgca
    12661 gaaccgcacg ccgggcctct ccggcatccc cgtcctcggc aacctcttca agcgccgcac
    12721 gacccggcgc gacaccgacg agctgctgtt cttcatcacg ccgcgcatct atcgtcccga
    12781 ctatcagggc cggcgcgtcg agtccagcac acagacgggc acgcgcttcg tctcgatccc
    12841 gcagcccgtg ccgctcggca accccgagac gaacacgccc acgcagcagg atcagttgca
    12901 gcagcagaat cagcagccgg cgccgatcat cattcccatg ccggcgaacc cgcagcaggg
    12961 ccagtcgcag accaccacgc cgccgtccca gaccggcgaa acgccccgac cgtaaaggag
    13021 gtttggaaaa gatgttcagg gaagcactcg aaagcattaa cgggcgcacc gagggagcac
    13081 tcggctctct catcatgggc acggacggca tcgcggtgga aaaacttctg ctcgaagacg
    13141 gcatcgaggc caacatcgat gtcgccgccg ccgaggtcac ttcgctcgtc cgcagcgcgc
    13201 aacgggccgg acgcgacacc gggctggggg ccgtgcgcga actcatcctc accttcgagg
    13261 ggatgaacat catcgcccgg ctcatcacgg ccgactattt cgtcgtgctg gcgctccaac
    13321 ccgacggcaa cctcggccgc gggcgcttcg agctacgcaa ggccgagatg gacctcgcgc
    13381 gcgagttcgc catgtagagc gccgactttt acgagcaagg ggagggtcgc gaaaaggggc
    13441 cgcgtcgaaa ggcgcggccc cttctccgta aggcaaaagt aaaaaggcaa aaggcaaaag
    13501 tgaagaagca gaatcgcctt cttcactttt gccttttgcc tttttacttt tgccttaccc
    13561 cggcagcttc acgtgcggca tcccgcgcag gatggcgcgc tggcgacgct gcacgcgctt
    13621 caggttcgac ttggggttct gcgccgtcgg cacggggttg aagacggtgc gcgggttcgg
    13681 gatcatcgcc gagaggaagg cggcctcgcg cgcgttgagc gtggcggccg gcttgttgaa
    13741 gtaggtttgc gccgcggcct ccgccccgta gacgccgtcg ccccactcga tgacgttaag
    13801 ataaatttcc aggatgcggc gtttggggag cgagcgttcg aggaagtagg tgatgaacac
    13861 ctcgcggccc tttcgcgcga acgaacgctc cgaggagagg tagaggttct tggcgagctg
    13921 ttgggtgatg gtcgaggctc cgcgcttgaa gtgcgagagg tcgggaatcc agctttgctc
    13981 gtccgtctcg ccttcctctt tggcttcctt ctcggcggcg cggcggccct cctcccacgc
    14041 cttgcggatg gcttcgtagt cgaagccgtc gtgcgcggcg aagttcgtgt cctcaccggc
    14101 caggacggcg cgctgcaact cgggcgaaat cttctccagc ggcacccact gctgcacgcg
    14161 tctcggctcg cgcccctctt tgcgggcctc ctcaagccgc gtctcgatca tgctggtcgt
    14221 ctccgggttc tgcgtccgca gccgcgacac gcgcgggaag gtcacagcct cgtacacgat
    14281 ccagagcgtg aagagggcga acaggaagag ggcgaaagtc ttaatcagtc tccacataaa
    14341 aacggctgtc aatcgtcagt tgtcagtcgg tagtctgacg aacataactg tgtccgacgt
    14401 gcaaaatata acgaccgccg aaattataac gcatgtggcg gggtaacgag ttcgccttaa
    14461 atcgccgtgg agtccggccc cgcactttga gctttgagct ttgaaccttg aactccctcg
    14521 aagacggcct tcgccgcgcg aatggtgttg caatgaatct cgacggtgtc gtcgatgtcg
    14581 gcggcgtaac cgccggacat cacggtcgcc acgggtaacc ccgcgtcgcg gcaggcgcgc
    14641 aacaccaact cgtcgcgggc gcgcaagccg gaaatcgtga gcgcgaggcg gccgagcttg
    14701 tcgccagcgt atgggtctgc gccgccgagg tagaagacga tgtcgggcgc gtgctcgaag
    14761 acgcgtggca gatggcgggc gagcgcgtcg agatattcgt catcggccgt cgcatcgggc
    14821 agttccacgt cgagcgtgga ggcagccttg aagagcgggt agttcttcgc gccgtgcatc
    14881 gagaaggtat acacgtccgc gtctccggcg aagatggtgg cggtgccgtt gccctgatgc
    14941 acgtcgcaat cgacgaccgc cgcgcgccga atcaaaccgt ctcgacgcag cacgcggatg
    15001 gcgacggcca cgtcgttcag cacgcagaag ccctcgccgc gatccgcgaa ggcgtggtgc
    15061 gtgccgccgg caaggttcga gccgacgcgc tccgcgagcg cgcggcgcgc ggctccaagc
    15121 gtgccgcccg tcgcgaggaa cgagcgccgc acgagcgcct tcgaccacgg caggcccagc
    15181 cggcgcaact cgcgcggcgt gagcgccccg gcgcgcaggc gcgttacgta atcctcggtg
    15241 tggacgagca acacatcttc cacggccgcc ggtcgcggct cgacgatctc ctgcggcgcg
    15301 agtgtcccct cccgaagcaa acggtcgcgc accatctcga acttccgaat cgggaagacg
    15361 tgcccctcgc cgatgtccgc gtagtagcgt ggtgtgtaaa aaactttcat tcgtgactca
    15421 cgtgtggtgg agtgatattg ccggaagatc gtttgagctt caatcgccgc ggattggtaa
    15481 tgcgaaggcg tctccctgat tccggcgccc accccgtgtg acccgaacgc gccgcagccc
    15541 gacggctgct actgcgacgg ccggctgcgg ctcaggcatc cggtcgtcgt cgcctgagga
    15601 tcggtcttga ccgtcagagt tgacgggcgt ataagtcggg cgtcgccccc tcacagcttc
    15661 cgttggtctg accctaatgc ccgcacagga gaagcctcat gaatcctcaa ctctcaccta
    15721 cgcctcaaag aagatttgct cttcaggtgg cgctgctggc cgcgctcgcc acggtgtgcg
    15781 gcggcgtcat catttcaggc acggcccaat cgacgaaggg ggagcgggag tttaagaaca
    15841 cggtgcccgc gcacgtgccc gtcaaggtga agctgaagaa tgaacagtcg ttcaaggacg
    15901 cgaagaataa gaactgggcg cgtgggctgg aaatagaggt caagaacact ggcagtaagc
    15961 cgatttactt tctgtatatg gcagtcgtca tgccggattt cgtactcgaa gatggaaacc
    16021 cggcgggctt tcagttgacg tatggcagaa aggatttagt aatccttagt actccgattc
    16081 agcctgacga cattccgata atgccgggcg acagcgtcac cctgaaagtc tccgccagtc
    16141 agctcaaggg cttcgagggt ctgagggatg aaaaaaagtg gccggacccg aagcgggtca
    16201 gattcgacat gcagctcatc aacttcgggg acggtacggg gctccggtct actcaagggc
    16261 taccgttggc gcgtccgggt aaaagagttt cccagaccac acccaccctg atggactggc
    16321 tggacgtggc gccgcgcgct cgcgatgttt acgtggatga tgcgtctgtc aggtttgata
    16381 aaattgattg ccccacgtcg cccgcgagct tcttgcgggt aaatttttat cccccgaaca
    16441 acatcaggca cttacgagtc gctgatttat gcggatgcca gaacagcaat gattgtatgt
    16501 gggggtacat cggcttcccg agctgtcctt gtgacgaccc gggggaattt ttcggtgtca
    16561 tgccgggtgg agggtgcgcc aatccctatg cgggctgttt gagagtcgaa actgtaaatc
    16621 ggttttgcga cactcagtac aatggcagac agcagtgtca attccaggag gacgtgggga
    16681 attgcgccat cggcgacccg acgccgacgc cgacgccgac gcccggcccg gcgggcggcg
    16741 actgcgaccc ggcggcgcgg cccacctgcc cctactccga ctgcaacctc aggaccggcc
    16801 agtgggagtg ctgcgaccag aacacgcgcc ccaactcgaa ttgcccctgc gagtattcca
    16861 acctgtcggg gagcgtcgag tgggagtgtc acctcaactg cctcgcctcc ggctacagcc
    16921 cggcaaactt ccaacactac cgggccacgg gctgtgcgcc gaactcctac aacgagggca
    16981 actgctgcaa gtgctacaac cagaatccga cttgcccgtc ggggttcacg tgggacaagt
    17041 ctcagtgcaa atgctgtaac tcgtcggggc aatgcaccga aaccgttgcc ggtggcggcg
    17101 gtagcggctg cgacaacccg ccgctgcccg acggcacctg tgccgacggc agctatgtcg
    17161 atgccacagg gtgctgcggc ggcgactgga cttgcaaccc gtactgctgc cagacggggg
    17221 aggggtgcaa taacttcggc gaccccgagc cgccgcccct cacagacatc cttaacgact
    17281 cgccgtcgct ggccccgaac ccgaacgacg actgctgcga ctacagcccc atcctggtgg
    17341 acgtgtccgg cgatggcttc gacctcaccg gcgcgcacgg cggcgtccgc ttcgacatgc
    17401 ggggcgacgg ccgcccggcc gagatgtcct ggacgcgctc aggctccgac gactcgtggc
    17461 tcttcctcga ccgcgacggc aacggcgttg tagataacgg caaggagttg ttcggcaact
    17521 acaccgagca gccgcgctcg gtgcggccca acggcttccg cgccctcgcc cagttcgaca
    17581 aggccgagcg gggcggcaac ggcgacgggt ggattgacag ccgtgacggc gtcttcgcct
    17641 ccttgcggct gtggcgggac gccaatcata acggcgtctc cgagccgggc gagctgcaca
    17701 ccttgccgtc gttcaaagtc gtctcgttct cgctcgacta caagacttca cttcagcgcg
    17761 accgacacgg caaccgctac aggtacaggg cgcccgtccg catcgacgac gggcgtcggc
    17821 tcttcggccg ctgggcgtgg gacgtgttcc tcgtcgaagc acagtagccg aagcccacgc
    17881 tacagagaga ccgcccggaa gtctgtgatg acttccgggc ggtcttcaca tcaggcgaca
    17941 atcaatactg aatcaagctg ccctcgcgct tggcgcggcg gacggcgcgg gagaagaccc
    18001 agagcgagaa ggggagaagg gcggcgagga agatgaggag cgtgacgagc gggtcgcgca
    18061 cttcggcgaa gccttgcccc tggatgagta cgcggcggat gccgtcgatg ccgtaggtcg
    18121 tggggaagag gctggagacg aggccgaagc ccttgccggc gacggaggag agaagctgcg
    18181 tcgggaagaa gacgccggag aagagggcgg agccggtgcc gatgatgacg ccgaaggggt
    18241 cgccgcgctt gaagaccatc gcgaagctcg ccgagaggat accgatggag gcgagcacga
    18301 gtgtcgtgag caggagaaac aggagcgcga ggaggaagtt gccgcggtag tggacgccga
    18361 agagcaccca gccgaagaag aggtagagga acgaatggaa ggtcgccagc acgaagtccc
    18421 aggcggagct ggcgatgatg agcgtcggca cgttgatggg cgtcatcagg acgccttcga
    18481 gcgtcccctg cgactgctcg gagcggatgg cgttggcgag gctggagaag ccgaccatga
    18541 aatagttgag gaagggaagc gcggtgatcc aggcgaggaa ggggtcgcgc cactggtcgt
    18601 aaggggtgtc gaagcgcccc gtgaaaatcc gcgagatgaa gtagagcgtg gtggtgacga
    18661 agaggatcga catgatccgc atgaagaact ccagccggta gctcaccgcg agctggaagt
    18721 cgcgcaccac gaaggcgcgc acgcgcccga gcatcgacag cagtcgcctc aacctctcac
    18781 ctccgccgcg cccgcggccg tcgtcgccgc ggggccgtcc gactccttct cgtaacgctc
    18841 gatcacgtcg agcagcgtcg cccgctccga ctcgcaggag acgacgcgcg cgccgtccgc
    18901 gagcagcagc ttcaaggcgc ggtcgagact gtcgtcggaa gtctcgcgca tgaaattgac
    18961 ggcgacactt ccgccgcctt cgtccgagac
//
LOCUS       DASVES010000526         5019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1481579, whole genome shotgun sequence.
ACCESSION   DASVES010000526 DASVES010000000
VERSION     DASVES010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481579"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF240_22475"
     CDS             <1..272
                     /locus_tag="VF240_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178040.1"
                     /translation="DIEYAFYMVGGGGLHIYEGTNYRGQFGTYVIGDIMRVAIEGGQV
                     KYYRNGSLLYTSTIAPAYPLLVDTSLNTVNAYIGNVVISGRQLSP"
     gene            320..739
                     /locus_tag="VF240_22480"
     CDS             320..739
                     /locus_tag="VF240_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178041.1"
                     /translation="MDIRKHIGETTSGLTQSGGRRSLLALACLLILGAGAITATALSC
                     ASTQQPEEVRTVLTPTGFEPPEATRTAGRIRLRVTNQSGAGELTLRLTLVDGGLVGEA
                     RVPSGAAEWVGEFELSAGKYVLTEASNPAWLFYVIVQ"
     gene            1035..>5019
                     /locus_tag="VF240_22485"
     CDS             1035..>5019
                     /locus_tag="VF240_22485"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178042.1"
                     /translation="MARVNRTVYYHFALRLVGVCAFMSALFAVNTFAQMSVTSSAADA
                     RTPSGMESGSGTINPYNGNLTYVLPLLQLGGRGEVDYTINLLLEQKWTVLTTVSNYNC
                     INNPNCSGPISRSSAKFNWTPGLKPGFAPGVMQGRQAGNFQYESTGNYGNEFAGLGHR
                     LLPPYNGISHPRVIGTQNTLTRLVFTANNGTEYELVDKATEGRPVSASCDAQTPGYNR
                     GRTFVSRDGTSMTFVSDANILDLACSQATLYGEVRPENYVFNVSGHLYTKNGAVYRID
                     GGRVSSIRDRNGNYMRFSYDPAGNLDVVTDSMGRQVTFNRQSTYTQIDYKGFGQPRSI
                     RVHWGRLETVLRPHYQMKTLYDLFPLFRYDNNTPENFNPTLASAVELPDGRRYNFYYN
                     SYGELARVVSPTGGAVEYDFAAGVEKLGLNPEIDVTSGMATGTHWAAIYRRVIEQRAY
                     PKGGAAGNYENKLTYSRRRETFTPASGGGYNLISIENDGFVFEDQRDVNGVLYARRKH
                     YYHGDPLDSQTESYFNPAGYSYWNEGREYKTEEFATDGVTILRRVESEWKQRAPVGWW
                     QSFCASVQALNYCYPNAAAVEPPNDVRVTRTTVTLVDGNLVSKQEFAYDDFNNQTDVW
                     EYDYGVGTLPAHPVRHTHTEYLGTNAGIDYSATPVHIRHLPRAQQVYSVDPGTGVETL
                     AAQSETRYDEFSLSTYGNIVGWEDPGSVARGNPTTARGWLNTNNTWLETRAEYDQCGN
                     ALKSYDARGKVSESEYSDAYKYAYATRSFSPVPDPTGARGSSSALETRIVYDLETGLA
                     TSKTDANGQVIGFEYDLLSRLKTVTPPSGGNQVIHEYGDSPGNLFAKVKKQIDEAVWD
                     EAVTYFDGFGRTIKTLSHDARGDVFSETLYDPVGRVKKTSGAYRTGETKNWVETIYDD
                     HGRIKEVIAPKINNEAAPARVKGEYSSATVGGQIGLVHVGVNQAGKKARSITNALGQL
                     IRVDEPNQDNELGPIDNPFQPTYYKHDALGNLRKVTQGGQVRYFLHDSLGRLIRVRQP
                     EQDTNPALAVSDPVTGNSQWSTDSTYDNNGNVLTATDAKGVTVNYEYDNLNRVVQRSY
                     SITQTTDPKKVTFATPTVTFKYDGILSPTPDNPNPTVVPLAKGLLTEISNGISTTQHT
                     SFDNLGRIKSSRQIVDGQVYSFPSYKYNLAGALLEQTYPSGRVVTYAFDGSGDLSSVS
                     SNLPNQTPKLYASYLRYTANGSVDQLKLGNGRWETYQFNAREQVIQIGLGVSATDTSL
                     WSVDYEYGRLNPDGTVDVTKNDGNVVRQTITVPGVAYPYVQTYGYDSLNRLSEAVETY
                     GGLQTWRQ"
BASE COUNT         1152 a         1489 c         1429 g          949 t
ORIGIN      
        1 cggacatcga gtacgccttc tacatggtcg gcgggggcgg cctgcacatc tacgagggca
       61 cgaattatcg ggggcagttc ggcacctatg tcatcggcga catcatgcga gtcgccatcg
      121 agggcggcca agtgaagtac taccgcaacg ggtcgctcct ttacacgagt accatcgctc
      181 cggcctaccc gctgctcgtg gacacgtcgc tcaacaccgt gaacgcctac atcggcaacg
      241 tcgtcatctc cggccgccag ctctcgccct gacggatggc atccggcttt ttcttcatgt
      301 tttcgacagg agtgaataga tggacatccg caaacacatc ggggaaacga cttccgggct
      361 gacccaaagc ggggggcggc gctcactgct cgcgctcgcc tgccttctta tcctcggcgc
      421 cggcgccatc accgccaccg ccctaagctg cgcgagcacg cagcagcctg aagaggtgcg
      481 gacggtgctg acgcccacgg gcttcgagcc gccggaggcg acgcgcactg ctggcagaat
      541 ccggctgcgg gtgacgaacc agagcggggc cggcgagctg acgctgcggc tcacgctggt
      601 cgacggcggg ctggtgggcg aggcgcgggt gccgagcggg gcggccgagt gggtcggcga
      661 gttcgagctg tcggccggca aatacgtcct gacggaggcc agcaacccgg cctggctctt
      721 ctatgtcatc gtgcagtagg agatcggtat aacttatgcg gccgggagcc ggcgttattg
      781 agttagtgtg taacactcct caaactcccc aatctgtccg cgcgtaaatc tcgctcctgg
      841 ttctctactg acatgaagca ccactgccct ttccgaacta tgcatcaatt gcacagtgag
      901 ataattgcgt cccaattagc tgagcacgaa agtttatggt ttatcggtga aggctcacgc
      961 ttaaaagtga gagcaagttc ttaggtttga acgctccgtc aaccctaatc cgcccttcgt
     1021 cccggagaga agacatggcc cgcgttaata gaacagttta ctaccacttc gccctgcgtc
     1081 tggttggggt ctgcgccttt atgtctgcgc tgttcgctgt caacacgttc gcgcagatgt
     1141 ccgtcaccag tagcgccgct gacgcgagaa ccccgtccgg catggagagt ggctccggga
     1201 ctatcaaccc atacaatgga aatctgacct acgtcttacc acttctccag ctcggcgggc
     1261 gtggagaggt tgactacacg atcaacctgc tcctcgagca gaagtggacg gtcttaacca
     1321 cagtctccaa ttacaactgt atcaataatc ccaactgctc cggtcccatc agcaggtctt
     1381 ccgcgaaatt taactggact cccggcctga agcccggttt cgcgccgggg gtgatgcagg
     1441 ggcggcaggc aggcaacttc cagtacgagt ctaccggcaa ttacggtaat gaattcgcgg
     1501 gcctcgggca ccgcctgctc ccgccctaca acggcattag ccacccacgg gtcataggca
     1561 cacagaacac gctcacccgc cttgtcttca cggccaacaa cgggacggaa tacgaactcg
     1621 tggacaaggc caccgagggg cgtccggtct ccgcatcctg cgacgcacag acccccggct
     1681 acaaccgcgg tcgcaccttc gtcagccggg acgggacatc gatgacgttc gtctcggatg
     1741 caaacatttt agacctggca tgcagccagg ccaccctcta cggtgaggta cgccccgaga
     1801 actacgtgtt caatgtttcc ggccacctct ataccaagaa cggtgcggtg tatcggatag
     1861 acggggggcg cgtttcgtcc atccgcgacc gcaacggcaa ttacatgcga ttcagttatg
     1921 atccagccgg caacctcgac gtcgttaccg acagcatggg caggcaggtg acattcaacc
     1981 gccagagcac ttacacgcag attgactaca aggggttcgg gcagccgcgc tcgattcgcg
     2041 ttcactgggg caggcttgaa accgtcctgc ggccccatta ccagatgaag accctctacg
     2101 accttttccc gttattccgg tacgacaaca acacgcccga aaatttcaac ccgaccctgg
     2161 cctccgccgt cgaacttccc gacggcagac gttataactt ttattacaac tcctacggcg
     2221 agctggcgcg ggtcgtgtcg ccgacgggcg gagccgtcga gtacgacttc gccgccgggg
     2281 tggaaaagct gggcctcaat cctgaaatag atgttaccag cggcatggcg acaggcactc
     2341 attgggcagc catctaccgc cgagttatag aacaaagggc atatccgaag ggcggggcgg
     2401 ccggcaatta tgaaaataaa ctcacttaca gccgcaggcg cgaaactttc accccggcct
     2461 ccggcggcgg ctataaccta atctcgattg agaacgatgg tttcgtcttt gaagatcaac
     2521 gagatgtgaa tggagtcctg tacgctcgta gaaagcacta ctaccacggc gacccgctgg
     2581 actctcagac agagtcctat ttcaaccccg caggttattc ctactggaat gaaggccggg
     2641 aatataaaac cgaggagttc gcaacggacg gcgtgactat tctccgccgg gtcgaaagtg
     2701 aatggaagca gcgcgcccct gtgggctggt ggcagtcttt ttgcgccagc gtgcaggcgt
     2761 tgaactactg ctacccgaat gccgccgcgg tcgaacctcc gaacgacgtg cgggtgaccc
     2821 gcacaactgt cactcttgtc gatggcaact tggtcagcaa acaggaattc gcctacgacg
     2881 acttcaacaa tcagactgac gtttgggagt acgactacgg agtcgggacg ctgccggcgc
     2941 acccagtcag gcatacgcac accgaatacc tgggcacaaa cgctgggata gactactcgg
     3001 caactccggt tcacattcgc cacctgcccc gcgcacagca ggtgtattca gttgatcccg
     3061 ggacaggtgt cgaaacgcta gccgcgcaaa gcgaaacgcg ttatgacgag ttcagcctct
     3121 ccacctacgg caacatcgtg ggttgggaag accccggctc ggttgcccgc ggcaacccga
     3181 ctaccgcccg aggttggctg aacacgaaca atacttggct ggaaacgcgc gccgagtatg
     3241 accagtgcgg taacgcgttg aagtcctatg acgcgcgcgg caaagtctcg gagagcgagt
     3301 attccgacgc gtacaagtac gcctacgcga ccagatcttt ctcccccgtt cccgacccga
     3361 caggcgcgcg tgggtcgagt tccgccctgg agacgaggat cgtttacgac ctggagacag
     3421 gcctggcaac ttcaaagacc gatgccaacg ggcaggtcat cggattcgag tatgatctgc
     3481 tctcacggct caaaacggtg acaccgccga gcggcggcaa ccaggtaatt cacgagtacg
     3541 gcgacagtcc cgggaattta ttcgccaaag ttaagaagca gattgatgag gccgtgtggg
     3601 atgaagccgt cacttacttt gacggcttcg gcagaaccat taaaacgctt tcccacgacg
     3661 cgcgtggcga tgtatttagc gaaacgcttt atgacccggt gggacgtgtt aagaagacca
     3721 gcggcgcata tcgcacgggg gagacgaaaa attgggttga gacgatttac gacgatcacg
     3781 gcaggattaa ggaggtcatt gcccccaaga ttaataatga agccgcgccc gccagggtga
     3841 agggtgagta ttcgtcggcc acggtcggcg gtcagatcgg cctcgtgcac gtcggggtca
     3901 accaggctgg aaagaaggcg cgctccatca ctaatgcact cggtcaactc atacgtgtcg
     3961 acgagccaaa tcaggataac gagttgggcc cgattgataa tccgtttcaa ccgacctatt
     4021 acaaacacga cgcactcgga aacctgagga aggtgacgca gggcggccag gttcgatact
     4081 tcctgcacga ttcactcggc aggctcatta gggtcaggca gcctgagcag gacactaatc
     4141 ccgccctggc ggttagtgac cccgtaaccg gcaatagcca atggtctacg gattcaacct
     4201 atgacaacaa cggcaacgtt ctcacggcga ccgacgctaa aggcgtcacg gtcaattacg
     4261 aatatgacaa tttgaaccgc gtcgtccagc gcagctactc gatcacacag accacggacc
     4321 cgaagaaagt cacatttgcc accccgacag tcaccttcaa atacgacgga atactgagtc
     4381 caacgccgga caaccccaac ccgacagtag tcccgctcgc caaggggctt ctaaccgaaa
     4441 tatcgaacgg catttccacg acacagcaca cgagctttga caatttaggc agaataaaat
     4501 cgagccggca gatcgttgat gggcaggtgt attcgttccc gagctacaag tataacctgg
     4561 cgggggcgtt gcttgagcag acgtatccat cgggcagggt agtcacttac gccttcgacg
     4621 gcagcggcga cctgtcctcg gtcagcagta atctgccgaa tcaaactccg aagctttacg
     4681 cgagttattt aaggtacacg gctaatggga gtgtggatca gctgaagctc gggaacggca
     4741 ggtgggagac ctaccagttc aacgcgcggg agcaggtcat acaaattggc ctgggagtca
     4801 gcgccacgga cacgagtctg tggagtgtgg attacgagta cgggagactc aacccggacg
     4861 ggacggtcga tgttacgaag aacgacggca acgtggtcag gcaaacaata accgtgcccg
     4921 gcgttgctta cccttacgtg cagacctacg ggtacgactc cctcaaccgc ctgtccgaag
     4981 ccgtggagac gtacggcggt ctgcaaactt ggagacaga
//
LOCUS       DASVES010000527         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1523036, whole genome shotgun sequence.
ACCESSION   DASVES010000527 DASVES010000000
VERSION     DASVES010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523036"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VF240_22490"
     CDS             <1..664
                     /locus_tag="VF240_22490"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178043.1"
                     /translation="AIAATRDGAGAVGRRHVEYARDKILMGVERLGFMMDEDERYATA
                     VHEAGHVAVGLGVKNGDPIHKVSILPRGRALGVTQALPERDRLMKKREYLEDQIAMLL
                     GGRAAEMLLLDTMTAGASNDIERAVEIARKMVSEFGMSPLGPIHLGKPEDPHSQALLD
                     RVENATNEIIDVQLRRACEEVDLRRKEIALLVEGLMERDTLDSDEILTCFGVSNPEKA
                     AA"
     gene            880..1611
                     /locus_tag="VF240_22495"
     CDS             880..1611
                     /locus_tag="VF240_22495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178044.1"
                     /translation="MAYPGVDRIELPLLQELVATGGVDDVHFLYDRLVAYFPQLDEAD
                     VRALANGHRLQWRRIVQRAGRALEEKRQIERRRGRWTITTAGRRRVTDEDLHFDFSEE
                     GSREPATSPEITHADVQQMLIEIGRILGFHAQSEFDYYDVVWREGAESPRLSHVFEVQ
                     HRGNVDAALAKLKRAYDAQRTRPFLVVSSERDTNRAEMQMSLARTGAFHEIGSVTTIL
                     SFEQLRRLHRALTSVADILPLIFAR"
     gene            1608..>2554
                     /locus_tag="VF240_22500"
     CDS             1608..>2554
                     /locus_tag="VF240_22500"
                     /inference="COORDINATES: protein motif:HMM:NF013765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178045.1"
                     /translation="MNATPIRTQYLEVKRRYSDCLLLFRLGDFYEAFDDDARILAREL
                     DIVLTSRQMGRNTRVPLAGIPYHSLERHLATLIGKGHRVALCEQLTTAPVKGSGGGGR
                     GLIERDVVRVVTPGTVLEPGLLRGKVNNYLAAYVSDGRRAGIAHADVSTGDFSATEVE
                     ESAALAELHRIGPSELIVPRGFNRAEAGLPGFITRRPDSAFEHMQAKRELQQHFGTRT
                     LAAYGLARWPLATTAAAAIVAYLRETQPCSAEQLTRLTSYHTANYMVLDEHTVRSLEI
                     FESSGGAPSLLSTLDETRTAMGGRLLRRWLRQPLLDGVE"
BASE COUNT          455 a          869 c          808 g          422 t
ORIGIN      
        1 ggccatcgcc gcgacacgcg acggggccgg agccgtgggt cgccggcacg tcgagtacgc
       61 gcgcgacaag attttgatgg gagtcgagcg gctcggtttt atgatggacg aggacgagcg
      121 ttacgcgacg gccgtccacg aggcggggca cgtcgccgtc ggcctcggcg tgaagaacgg
      181 cgaccccatc cacaaggtct cgattctgcc ccgtggccgc gccctcggcg tgacgcaagc
      241 cctccctgaa cgcgaccgcc tgatgaagaa gcgcgagtac cttgaagacc agattgcgat
      301 gctcctcggc gggcgcgccg ccgagatgct tctgctagat acgatgacgg ccggcgcgtc
      361 gaacgacatc gagcgggcgg tcgagatcgc gcgcaagatg gtctcggagt ttggcatgag
      421 tccgctgggg cccattcacc ttggcaagcc ggaagaccca cacagtcagg cgctgctcga
      481 ccgcgtcgag aacgcgacga atgagattat cgacgtacag ttacgtcgcg cctgcgagga
      541 agtggacttg aggcgtaagg agatcgccct tctggtcgag gggttgatgg agcgcgacac
      601 gcttgattcg gacgaaattc tgacgtgctt cggcgtctcc aatcctgaga aggcagcggc
      661 ctagtcgtcg ctggcgcaag ttaacatcgg aggcggttgc ggggatgagg agaaatccgg
      721 ccccgttacc gcctccgatt tttgtgtcca ctgccgaacg cggacgccgc caagcttgcc
      781 tgacgccgcg tttctcttgt aactttgcgg tcgcgagggc gggggcagta gggtgccatt
      841 tcgcccgccc attctccaaa actcccgatg taaacgaaca tggcctatcc gggcgttgac
      901 cgcatcgaac tgcctttgct tcaggagctg gtcgccaccg gcggcgtcga tgacgtgcat
      961 ttcctctacg accgtctcgt cgcgtacttc ccgcaactcg acgaggcgga cgtgagggcg
     1021 ctcgccaacg ggcaccgcct ccagtggcga cggatagtgc aacgcgcggg acgcgccctc
     1081 gaagagaagc gccagatcga gcggcgccgg ggtcgctgga cgataacaac ggccgggcgc
     1141 aggcgggtaa cagatgagga tttacatttc gacttctctg aggaggggag ccgtgagccg
     1201 gccacttcgc cggaaatcac gcacgccgac gtgcagcaga tgctcattga aatcggccgc
     1261 atcctcggct tccacgccca gagcgagttc gactactacg acgtggtctg gcgcgaaggg
     1321 gccgaaagcc ctcgcctcag ccacgtcttc gaggtgcagc accggggcaa cgtggacgcg
     1381 gccctcgcca agctcaagcg cgcctacgac gcgcaacgca cgcgcccctt cctcgtcgtc
     1441 tcaagtgaac gcgacaccaa ccgggccgag atgcagatga gtctggcccg caccggcgcc
     1501 ttccacgaaa tcggcagcgt cacaaccatc ctcagcttcg agcagctccg acgactccac
     1561 cgcgccctca cttccgttgc cgacatcctc ccgctaatct tcgcgcgatg aacgccacgc
     1621 ccatccgcac gcagtacctt gaagtcaagc ggcgctatag cgactgcctg ctcctcttcc
     1681 gtctcggcga cttctacgag gcgttcgacg acgacgcgcg tatcctcgcc cgcgagttgg
     1741 acatcgtcct cacgtcgaga cagatgggac gcaacacgcg cgtcccgctc gccggcatcc
     1801 cctatcacag ccttgagcga cacctcgcga cgctcatcgg gaaagggcac cgcgtcgccc
     1861 tctgcgagca gttgacgacg gcgcccgtaa agggcagcgg cggtgggggc cgggggttga
     1921 tcgagcgcga cgttgttcgg gtcgtcactc cggggacggt gctggagccg ggtctgctgc
     1981 gcggcaaggt caacaactat ctggccgctt atgtgagcga cggccgtcgc gccggcatcg
     2041 cccacgctga tgtttccacc ggcgacttct cagccacgga ggtcgaggag tcggccgcgc
     2101 tcgccgagct tcaccgcatc ggcccttcag aactcatcgt cccgcgcggc ttcaaccgcg
     2161 cggaggcggg cctgccgggt ttcatcacgc gccgccccga ctcggccttc gagcacatgc
     2221 aggcgaagcg cgagctgcaa cagcacttcg gcacgcgcac cctcgccgcc tacggcctcg
     2281 cgcgctggcc gctcgcgacg acggccgccg ccgccatcgt cgcctatctg cgcgagacgc
     2341 agccctgctc tgccgaacaa ctgacgcggc tcacgagcta tcacaccgcc aactacatgg
     2401 tgctcgacga gcacaccgtt cgcagcctcg aaatctttga aagctccggc ggcgccccgt
     2461 cgctcctctc cacgctcgac gagacgcgca cggcgatggg cggacgcctc ctgcgtcgtt
     2521 ggctgcggca accgctgctc gacggcgtcg agat
//
LOCUS       DASVES010000528         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_901208, whole genome shotgun sequence.
ACCESSION   DASVES010000528 DASVES010000000
VERSION     DASVES010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901208"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..764)
                     /locus_tag="VF240_22505"
     CDS             complement(180..764)
                     /locus_tag="VF240_22505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178046.1"
                     /translation="MEYPLQLSFKLLAIARQISVADAHGQLVLYVKQKAFKLKEAMTV
                     FADAAQTRSLYTIGADRILDISARYNFADSAGNYLGSIKRQGMRSIWKAHYDVFDGDR
                     LELTITEESAWTKVLDSLVGEIPVVGMFTGYLFHPAYLVKRTNGAVVLRLEKRPALFE
                     GKFTVTKHGDLSPPEETRALLAIITTVLLERGRG"
     gene            complement(812..1177)
                     /locus_tag="VF240_22510"
     CDS             complement(812..1177)
                     /locus_tag="VF240_22510"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7178047.1"
                     /translation="MKHTEGFLKIVDEARARVREVSVEEARRRLSENTSARLIDVRED
                     NEWEAGHASGAEHMGRGIIERDIETRHPDHDAELILYCGGGYRSALACDNLQRMGYRQ
                     VYSLAGGWKAWKDAGAPVE"
     gene            1288..1752
                     /locus_tag="VF240_22515"
     CDS             1288..1752
                     /locus_tag="VF240_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178048.1"
                     /translation="MRKNLVSCALALFVSAAAMGCRTGPASQGGANAGGGPDAGVVGG
                     AVAEVNSFTDELLKKIDSAQDPAAGADEAQAFLDARKVEMKARLDAARASREFQESAE
                     ARGRLLDAEVTNIDRVSSLRTRHIDRWMRDPQFKAKLDRLVADYDALWPRAG"
     gene            1936..>2609
                     /locus_tag="VF240_22520"
     CDS             1936..>2609
                     /locus_tag="VF240_22520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7178049.1"
                     /translation="MSTEQFDVTIIGAGPGGYVAAIRGAQLGLRVALVEKDKRLGGTC
                     GLRGCIPTKQLLMSAHIYEQMQHAKEFGVQASEIKLAFADVQKRKDKVVTKNSAGVSY
                     LMKKNKVTVFSGSGKLALPGKVEVTGADGKTETVNTKNIIIATGSVVRPIPGFETDGE
                     RIVNSDHILELKEVPKSLVVLGSGAVGTEFASVYSRFGTETTLVELMPRLMPLEDEEV
                     SKELEKS"
BASE COUNT          475 a          774 c          877 g          483 t
ORIGIN      
        1 gggcttatgc gaataacttt catcaaagta gccgacaagc atagccatca acctttcctt
       61 cacgcggaaa ggaaacacag cactagctaa gcgccttaag gggtcgtatg gcctatgatt
      121 attttgtgtg acgcatggtg cgtcaactat tttatcaccc taatctgggc cataacccct
      181 tacccgcggc cgcgttcgag gaggacggtg gtgatgatgg cgaggagggc gcgggtctct
      241 tcgggggggg agaggtcgcc gtgtttggtg acggtgaatt tgccttcaaa gagggcgggg
      301 cgcttctcta agcggaggac gacggcgccg ttggtgcgct tgacgaggta ggcggggtgg
      361 aagaggtagc cggtgaacat gccgacgacg ggtatctcgc cgacgagaga gtccagcacc
      421 ttcgtccacg cgctctcctc ggtgatggtc agctcaaggc ggtcgccgtc gaacacgtcg
      481 tagtgggcct tccagatgga gcgcatgccc tgtcgcttga tggagccgag gtagttgccg
      541 gcggagtcgg cgaagttgta gcgggccgag atgtcgagga tgcggtcggc cccgatggtg
      601 tagagcgagc gcgtctgcgc ggcgtcggcg aagaccgtca tggcctcctt gagcttgaag
      661 gctttttgtt tgacgtagag gacgagctgg ccgtgcgcgt ccgcgacgct gatctgccgc
      721 gcgatggcga gcagcttgaa cgagagctgt aaggggtatt ccatcgattt gttctcctcc
      781 aactgattct ccttcgcgcg cgccgcgcgt cctactcgac gggcgcgccc gcgtccttcc
      841 aggccttcca gccgccggcc agcgaataga cttggcggta gcccatgcgc tggaggttgt
      901 cgcaggcgag ggccgagcga tagccgccgc cgcagtagag gatgagttcg gcgtcgtggt
      961 cggggtgacg ggtttcgatg tcgcgctcga tgatgccgcg tcccatgtgt tcggcccccg
     1021 aggcgtgacc ggcctcccac tcgttgtcct cgcgcacgtc gatcaggcgc gccgacgtgt
     1081 tctcggagag acgccggcgg gcctcctcaa ctgacacctc gcggacgcga gcgcgcgcct
     1141 cgtccacgat cttcaggaac ccttcggtgt gtttcatctc gcctcccgcc cccgccgcgc
     1201 ggctcattgt cagccccgcc gggtttgact ttcaaattct cactttgcta catcataacg
     1261 cttcccgcgt gaggctgtgg agtcttgatg agaaaaaatc tcgtgtcgtg cgcgcttgcg
     1321 ctctttgttt cggccgccgc tatgggctgt cgcacaggcc ccgcctcgca agggggcgcg
     1381 aacgcgggcg gcggcccgga cgcgggcgtc gtcggcgggg ccgtcgcgga ggtcaactcg
     1441 ttcaccgacg agttgttgaa aaagatcgac tcggcgcaag acccggcggc cggggccgac
     1501 gaggcgcagg cgtttctcga cgcgcgcaag gtggagatga aggcgcgtct cgacgcggcg
     1561 cgcgcaagcc gagagtttca ggagagcgcc gaggcgcgcg gccgtctgct cgacgccgag
     1621 gtgacgaaca tcgaccgcgt gtcgtcttta cgcacgcgcc acatcgaccg ctggatgcgc
     1681 gacccgcagt ttaaggcgaa gctcgaccgc ctcgttgcgg actacgacgc gctgtggccg
     1741 cgagccggct gaattggagt tggggcgcgc gaccccgtat aatggcgttt gaaacagccc
     1801 aaacacatcg cgcactttcg ggccaaacgg cccggctccc cggcgggccg agagtgtgtc
     1861 gggccggatt tataagccgc gccgcccatg tctcaattac cgataaacgt actagagaga
     1921 gaggactccg aacaagtgtc aacggaacag tttgatgtga cgatcatcgg cgccggcccc
     1981 ggcggctacg tggcggccat ccgcggcgcg cagttgggcc tgcgtgtagc gctcgtcgag
     2041 aaagacaaaa gattgggcgg cacctgcggt ctgcgcgggt gcatcccgac gaagcaactg
     2101 ctgatgtcgg cgcacatcta cgagcagatg cagcacgcga aggagttcgg cgtgcaggcg
     2161 tccgaaatca aactcgcctt cgccgacgtg cagaagcgca aggacaaggt cgtcaccaaa
     2221 aattcggccg gcgtctccta tctgatgaag aagaacaagg tgacggtctt tagcggcagc
     2281 ggcaagctcg cgctccccgg caaggtcgaa gtcaccggcg ctgacgggaa gaccgagacc
     2341 gtcaacacca aaaacatcat catcgcgacg gggtcggtcg tccgtcccat ccccggcttt
     2401 gagaccgacg gcgagcgcat cgtcaacagc gaccacatcc ttgagctgaa agaagtgccg
     2461 aagtctctcg tcgtgttggg gtcgggcgcg gtgggcaccg agttcgcctc cgtctactcg
     2521 cgtttcggca cggagacgac gctcgtggag ttgatgccac gtctgatgcc gctcgaagac
     2581 gaggaggtgt cgaaggagct ggagaagtc
//
LOCUS       DASVES010000529         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_610992, whole genome shotgun sequence.
ACCESSION   DASVES010000529 DASVES010000000
VERSION     DASVES010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610992"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VF240_22525"
     CDS             complement(<1..1023)
                     /locus_tag="VF240_22525"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178050.1"
                     /translation="MHEEKDGKPRFNISERLEDLRREHEALKWEGSFRDYFELVSQQP
                     RIAQLSHARINDMVHAAGVEKLNPETRDEVTRYNFFASELYGIEEPISRIVEYFKSAA
                     QRLEVRKRILLLMGPVGGGKSTIVSMLKRGLEDWTRAEQGAVYAIKDCPMHEEPLHLI
                     PQGLRAEIEKHYGLYIEGDLCPQCRYVLEHTYQGRHEDVMVQRVLFSEKQRVGIGTFS
                     PSDPKSQDITELTGSIDLSTIGEVGVESDPRAYRFDGELNIANRGVMEFIEMLKCDEK
                     FLYNLLTLSQEQSIKTGRFAMIYADEVIVSHTNENEYIAFAGNRKSEALQDRIILVRV
                     PYNLRVS"
     gene            complement(1336..2295)
                     /gene="truB"
                     /locus_tag="VF240_22530"
     CDS             complement(1336..2295)
                     /gene="truB"
                     /locus_tag="VF240_22530"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7178051.1"
                     /translation="MIASMMEGILIVDKPDGWTSHDVVARARRLLREKRIGHTGTLDP
                     FATGVLVLLVGRATRLAQFLSGAEKEYEATIRLGYATDTGDLTGTPRGEARPCADTLR
                     EEIETALEHLRGEIEQVPPMYSAKKVQGKKLYELARKGEEVERKAVRVRVSEFEIMSD
                     KASLWLLANDDGTCDLRARVVCSAGTYVRALAESLGEQLGAGAHLSALRRTRAGEFRI
                     SGAHTLDELQTKTEAGAGAEILLPMKAALSGMPAVHLSGREAQKVRHGAAVGAPEPQP
                     EWPDGAHVSLFDEAGGLLAVGTFDAARGLVRPRVTLAAEENKH"
     gene            2429..4570
                     /locus_tag="VF240_22535"
     CDS             2429..4570
                     /locus_tag="VF240_22535"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7178052.1"
                     /translation="MKNNSALRLLLACVVTLLPFSQTARAQTTPYLSDDEIRMLSNEL
                     SGDRAFEHIRWLSHWHRDSGMEGYFKAAEYVQQAAKEVGLEEVRFVEQPLEGPNYTAR
                     SAELWMVEPVEVKLADIGEHNVYLADMSRDADLRAELVWIGDASEDALKGIDVKGKIV
                     LTSNQPGVAVQTAVWGKGAVGVVSFAPSESKNPVDYPDQIAWTRISSRVPEGKTGTFA
                     FALSPRKGEMLRRVLETKNEQDIFGTGKTTKGGRVVLKAKVDTEFGAAPGRTGYVEGV
                     IRGTKHNDQAIVLTAHLQEEKGSANDDGSGCGNLLEMARVFNKLIREGRIQRPLRDIK
                     FWWTDEIYSEYRHFSDHPEDARRILANLHQDMTGANQAIGSRVQHLIRAPHSRTSYLD
                     ALFESVGAYVINTNNGFLAASRQGGLPRPHTRPIYSSLGTRQGYNARFVPWFGSSDHM
                     VFLEGAIGIPAVALINWDDDFIHSSDDDLDLIDPTQLKRNAFIVASMAYFLAFADDAD
                     VPVIAGETYAQGSDRLANDLKVAMRLLKEGASDPAGSWKRAVYMIEQGVEREVRALES
                     ARVFAGTNPKATDTINQLVRRMTARAGELNSELETFYRQLHAAAPRTPSALTAEEQAA
                     QRKVPANVAPLADYFEKRGQARASGLRLHGLMRDEVYNFVDGRRTYYDIYKAVYAESQ
                     AAGTWYYGAVTLADVVKLLDAAVEAKALTLK"
     gene            4947..5528
                     /locus_tag="VF240_22540"
     CDS             4947..5528
                     /locus_tag="VF240_22540"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX7178053.1"
                     /translation="MRRRRPTPARDLRADMEEKRPARPIDAAKARARTMQRAAKLLAA
                     KPRSVAELRERLLEKSWTDEAAVDAALDKLKEYGYLDDERFAFGFASFRVRQKPVGRQ
                     RLARDLQLKKVPRETADAALDLVYEETPEEQLIDRAIEKRVRLRGRPSTRQETKSLFD
                     HLLRQGFSGDLVYRKVRAASAAEADETEESPAD"
BASE COUNT         1013 a         1974 c         1762 g         1024 t
ORIGIN      
        1 cgagacacgc aggttgtagg ggacgcgcac gaggatgatg cggtcttgca gggcctcgga
       61 cttgcggttg ccggcgaacg cgatgtactc gttttcgttc gtgtgcgaga cgatgacctc
      121 gtcggcgtag atcatggcga agcggccggt cttgatcgac tgctcctgcg agagcgtgag
      181 gaggttgtac aaaaacttct cgtcacactt gagcatctcg atgaattcca tgacgccgcg
      241 gttggcgatg ttgagttcgc cgtcgaagcg gtaggcgcgc gggtccgatt cgacgccgac
      301 ctcgccgatg gtcgaaagat caatcgagcc ggtcagctcg gtaatgtctt gagattttgg
      361 gtccgagggg ctgaatgtgc cgatgccgac tctctgcttc tcggagaaga gcacgcgctg
      421 caccatcacg tcctcgtggc ggccctgata ggtgtgctcc agcacgtagc ggcactgcgg
      481 gcagaggtcg ccctcgatgt agaggccgta gtgtttttcg atctcggcgc gcaagccctg
      541 cgggatgagg tgcagcggct cctcgtgcat cgggcagtct ttgatggcgt agacggctcc
      601 ctgctcggcg cgcgtccagt cttcgaggcc gcgcttgagc atcgagacga tggttgactt
      661 gccgccgccc accggcccca tcaggagcaa tatgcgcttg cggacttcca gccgctgcgc
      721 ggccgacttg aagtactcga cgatgcgcga gatcggttct tcgatgccgt acaactccga
      781 ggcgaagaag ttgtagcgcg tgacctcgtc gcgcgtctcg gggttgagct tttcgacgcc
      841 cgcggcgtgc accatgtcgt tgatgcgggc gtgggagagc tgggcgatgc gtggctgttg
      901 cgagaccagc tcgaagtagt cgcggaagct gccctcccac ttcagggctt cgtgctcgcg
      961 gcgcaggtct tcgagtcgct cgctgatgtt gaatcggggt ttgccatcct tctcttcgtg
     1021 catagccttc ttatgtacct ctcgtcggct tcgcgctcgt tggacggcgt ggggaaagtc
     1081 acgccctact tgttggactc ggtggacggt tggtgggagt gcggctcccg ccacttccgg
     1141 gccgcgtgcg gcagtcggcc tcgacgcgcg cgagaactta aggcgcacgc gcataagcgc
     1201 cgggcgcgcc gtcgaagctt ttccaaccgt tcaatgaacc ggctgagttt tgaactcggg
     1261 agattgtcgt tctgtgtcgg ggcggtccag cgcgcccgtc gcttacagac aagtataagg
     1321 cggcgggaca tcaagtcaat gcttattttc ctcggccgcg agcgtcacgc gcgggcgaac
     1381 aaggccgcgc gcggcgtcga acgtgcccac ggcgaggagg ccgcccgcct cgtcaaaaag
     1441 gcttacgtga gcgccgtccg gccactcagg ctgtggctcc ggcgcaccca cggctgcgcc
     1501 gtgacgcacc ttctgcgcct cccgcccgct tagatgcacg gccggcatcc cggagagcgc
     1561 cgctttcatg gggagaagaa tttcagcgcc ggcgccggcc tcggtctttg tttgcagctc
     1621 gtccagagta tgtgcgccgg agattctgaa ctcgcccgcg cgcgtgcgcc taagcgccga
     1681 gaggtgcgcg cctgcgccga gctgttcgcc gagcgactcg gccagcgcgc ggacgtatgt
     1741 gcccgcggag cagacgacgc gcgcgcgcag gtcgcacgtc ccgtcgtcgt tcgcgagcag
     1801 ccacaggctc gctttgtcgc tcatgatctc aaactcactg acgcggacgc gcaccgcctt
     1861 gcgttcgact tcttccccct tgcgcgccag ctcgtagagt ttcttcccct gcaccttctt
     1921 cgcggagtac atcggcggca cctgttcgat ctccccgcgc aggtgttcga gcgccgtctc
     1981 gatctcttcc ctcaacgtgt cggcgcaagg ccgcgcctcg ccgcgcggcg tgcccgtcag
     2041 gtcgcccgtg tcggtcgcgt agcccaggcg gatggtcgcc tcatactcct tctccgcgcc
     2101 cgagagaaac tgcgccagac gcgtcgcgcg gcccacgagc aacacgagca cgcccgtagc
     2161 gaacgggtcg agcgtccccg tgtgcccgat gcgtttctcc ctgagcagcc ggcgcgcgcg
     2221 cgccaccacg tcgtgcgaag tccacccgtc gggcttatcg acgatcagta tcccttccat
     2281 catcgaagca atcataaacg agcgccgccg tttccgcttc atcgcggacg ggcgtcgcgt
     2341 cgttggcttt acaacctggc gcgtctccgc gtttaatctg ttcctgccag cccttccaca
     2401 agtttttcta cagatacggg agatgaccat gaagaacaac tccgcactgc gcctgctgct
     2461 cgcctgcgtc gtcacgcttc tgcccttctc gcagacggct cgcgcgcaga cgacgcccta
     2521 cctctccgac gacgaaatcc gcatgctctc gaacgaactc tccggcgacc gcgccttcga
     2581 gcacatccgc tggctctcac actggcaccg cgactccggc atggagggct acttcaaagc
     2641 cgccgagtac gtccaacagg cggcgaagga ggtgggcctc gaagaggtgc gcttcgtcga
     2701 gcagccgctc gaagggccga actacacggc gcgctccgcc gagttgtgga tggtcgaacc
     2761 cgtggaggtc aagctcgccg acatcggcga acacaacgtc tatctcgccg acatgagtcg
     2821 cgacgccgac ttgcgcgccg aactcgtctg gatcggcgac gcctccgaag acgcgctcaa
     2881 gggcattgac gtaaaaggca agatcgtcct caccagcaac cagccgggcg tcgccgtgca
     2941 gacggccgtg tggggcaagg gcgccgtcgg cgtcgtctcc ttcgccccct ccgagagcaa
     3001 gaaccccgtg gactaccccg accagatcgc gtggacgcgc atctcctcgc gcgtccccga
     3061 aggcaagacc ggcacgttcg ccttcgccct ctcgccgcgc aagggcgaga tgctgcgccg
     3121 cgtgctcgaa actaaaaacg agcaggacat cttcggcacc ggcaagacga cgaagggcgg
     3181 gcgcgtcgtc ctcaaggcga aggtcgatac ggagttcggc gcggcgccgg ggcgcaccgg
     3241 ctacgtcgag ggcgtaattc gaggaacaaa gcacaacgat caggccatcg tcctcaccgc
     3301 gcacttgcag gaggaaaaag gctcggccaa cgacgacggc tccggctgcg gcaatcttct
     3361 ggagatggcc cgcgtcttca acaagctcat ccgcgagggg cgcatccagc gcccgctgcg
     3421 cgacatcaag ttctggtgga cggacgaaat ctattccgag tatcgacact tctccgacca
     3481 ccccgaggac gcgcgacgca tcctcgccaa tctccatcag gatatgaccg gcgccaatca
     3541 ggcgattggc agccgcgtcc aacacctcat ccgcgccccg cactcgcgca cctcttacct
     3601 cgacgcgctg tttgagagcg tcggcgccta cgtcatcaac accaataacg gcttcctcgc
     3661 cgcctcgcgg caagggggcc ttccgcgccc gcacacgcgc cccatctact cttcgctcgg
     3721 cacgcgacag ggctacaacg cgcgcttcgt cccgtggttc ggctcctccg accacatggt
     3781 cttcctcgaa ggcgctatcg gcatcccggc cgtcgccctc atcaactggg acgacgactt
     3841 catccactcc tccgacgacg acctcgacct gatcgacccc acgcaactca aacgcaacgc
     3901 cttcatcgtc gcctcgatgg cctacttcct cgccttcgcc gacgacgcgg acgtgcccgt
     3961 catcgccggc gagacctacg cgcagggcag cgaccgtctc gccaacgacc tcaaggtggc
     4021 gatgcggctt ctcaaagaag gcgcgagtga ccccgccggc agttggaagc gcgctgtgta
     4081 catgatcgag cagggcgtcg agcgcgaggt gcgcgcgctc gaatccgcgc gcgtcttcgc
     4141 cggcacgaac ccgaaggcca ccgacaccat caaccaactt gtccgacgca tgacggcgcg
     4201 cgccggcgaa ctcaactccg agcttgagac cttctaccgc cagctccacg ccgccgcccc
     4261 gcgcacgccg agcgctctca cggccgagga gcaagccgcg cagcgcaaag tccccgccaa
     4321 cgtcgccccg ctcgccgact acttcgagaa gcgcgggcaa gcccgcgcga gcggtctccg
     4381 cctccacggc ctgatgcgcg acgaggtcta caacttcgta gacggacgcc gcacctatta
     4441 cgacatctac aaggccgtct acgccgagtc gcaggccgcc ggcacgtggt actacggcgc
     4501 cgtcacgctc gccgacgtgg tcaaactcct cgacgcagcc gttgaagcaa aggcattgac
     4561 gctgaagtga gcgccggctg gcgcgttaat catgtatcgg ttaagtggga cgttgattcc
     4621 acgcgtagcg agccgctgta agcggcggca gaatgtagcc ccggtcgtga gaccggggaa
     4681 cagggtcaaa aaagacatga agccccggag gggcgaaaga accgcgccga tgccgagctt
     4741 gatattcatc gacgcgattc tttcgcccct ccggggcttt gattctctaa tcgtgttttg
     4801 tacccacggc tcgcgccgtg ggctaaaatc ttccgccgct ccgcggctca ttgacgcctg
     4861 cgcttctagc cactcgaact cccgtcatcg atcttcaaag cgcgcgtttg ccgcccgtca
     4921 cgcgcgcggg ttaaatttat ctcctgatgc gaaggagaag accaacgccg gccagagact
     4981 tgcgcgcgga catggaggag aagcgcccgg cgcgcccgat tgacgcggcg aaggcgcgcg
     5041 cgcgcaccat gcagcgcgcc gcgaagctgc tggccgccaa gccgcgctcg gtcgcggagc
     5101 tgcgcgagcg tctgctggaa aaatcttgga cggacgaggc ggccgtggac gcggccctcg
     5161 acaagctcaa ggagtacggc tacctcgacg acgagcgttt cgccttcggc ttcgcctcct
     5221 tccgcgtccg ccagaagccc gtgggccggc agcgtctcgc gcgcgacctc cagttgaaga
     5281 aggttccgcg cgagaccgcc gacgccgcgc tcgacctcgt ctacgaggag acgcctgaag
     5341 aacagctcat cgaccgcgcc atcgaaaaac gcgtccgcct ccgcggtcgg ccctcgacgc
     5401 gacaggagac caagagtctc ttcgaccacc tcctccgaca gggcttctcc ggcgacctcg
     5461 tctaccgcaa agtccgcgcc gcctccgccg ccgaggcgga tgagacggaa gagtcgcccg
     5521 ccgattaatc gtcgccgccg gctcgcacat ccgctcgttt agctcgcccc acttccacat
     5581 gctaaactta cgttttcggc acaccccact atttcccgct gacgggttcg gtggccgcac
     5641 tgccgggaag ttgatcgctg aatgctgacc gctgagagct ttttatgacc ggaaacgaag
     5701 ttagagaaat tttcctgaag tacttcgagc agcagggcca ccggcgcgtg ccctcaagcc
     5761 cgctgctgcc gac
//
LOCUS       DASVES010000530        10170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_852355, whole genome shotgun sequence.
ACCESSION   DASVES010000530 DASVES010000000
VERSION     DASVES010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852355"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..615)
                     /locus_tag="VF240_22545"
     CDS             complement(244..615)
                     /locus_tag="VF240_22545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7178054.1"
                     /translation="MELGAFSVSLAVKDIEASKVFYEKLGFTVFMGDQSQNWLIMKNG
                     DHAIGLFQGMFDKNILTFNPGWDSDAQPLGEFTDVRELQRRLKASGVNMISEADESST
                     GPASLMIADPDGNTILVDQHV"
     gene            complement(879..1313)
                     /locus_tag="VF240_22550"
     CDS             complement(879..1313)
                     /locus_tag="VF240_22550"
                     /inference="COORDINATES: protein motif:HMM:NF023374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HEX7178055.1"
                     /translation="MSAPLHDDSYSFKAEAAQKSRPRKPRRPPRAFSGSDETLARAFE
                     QRTSNIQVEGQGVVQRVLSDDNDGSRHQRFIVALASGQTLLISHNIDLAPRVVGLRKG
                     DVVSFSGEYEWNAKGGVIHWTHRDPGKRHAAGWIKHDGEVYQ"
     gene            complement(1501..3663)
                     /locus_tag="VF240_22555"
     CDS             complement(1501..3663)
                     /locus_tag="VF240_22555"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7178056.1"
                     /translation="MRRFSALVLSVSLAGSSFFLPYSATTRAQSKNPTVAPKARDEAV
                     PAKYAERLREFDEYVRRQMAADRTTALTIGFVKDGYVWVRGYGYADLENKLPARAESA
                     YRLASVTKPMTAVAVMQLAERGKLNLDDEVQKHVSYFPKKKWPVTIGQLLAHLGGISH
                     YRDFATEGHIKEHKDTRAAIAIFENFDLVAEPGTRYSYSSYGYNLLGAIIEAASGQSY
                     ADYMRENVWKPAGMSDTRMDDPLELIPNRVRGYQLVGGQLRNSEFIDISSRFAGGGTR
                     STVPDLLRFAVALNTGKLLAPASVARMYDTAATTAGRLTDYGLGWQTNPLNGRFLVAH
                     GGAQNETRTTLYNFPAQNFALAAASNFESANPDAYVMRLFQILTDEPLAVSAYTGDRA
                     KDALYRGLDDTYKWGLLHFARTAQPLSKTDAEAREAFTYFNTHMSADALAANAQEAAA
                     KLGAGRHPAAGQPFVKVGSLMAARLAERHGPARLNAYTGSGALSFFADYIALYKSRAD
                     YPAELKFSAPVERLIEQWSRGWARSYTDEVRRHSVSSASDFDALAPALKRAFAGAEIY
                     PNYLGDFAPAVRQLVHAGERERAFKAARLARELYPNSATANAYLAVTQLAYAEPSGAR
                     ELLLKAKEIDPTGVAGPGSLNSIAYDLAANGRRDAGLELLKLAAELYPKEANLYDSIG
                     EFYALKGQKDKAVEFYSKALEVDPNLASAKSALEKLKN"
     gene            3945..5135
                     /locus_tag="VF240_22560"
     CDS             3945..5135
                     /locus_tag="VF240_22560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178057.1"
                     /translation="MDIPRKAFLLMAWTLLATAGGPAAPLSRGLENSPRRCPQDAEEI
                     RVPSAASLICLEFDKKDVLVGSVNIPLTVQITSLKEKPLKLVDFSPADDNGTEKLIKF
                     AKSGEEELPLEGGVEVRSVYRFHFSIAENAEPRSYAIRVQLKYASKDPPPSAESFHLK
                     VGGSGAVRLADNTRTVSAMTGEITTQHLEVKGAYYYPVRLRQVTIYSDPADLVARVAI
                     PADVPIRNRKEVETIDVSFPVRKMELKDWISAFGSENKLWFEFEYEDDEKRVLKSRDA
                     LKLAVRPSVLALVSAILLGVLAGTIVKTDLKRLQVEGYITRRQKALFIVGTSVIGLIA
                     ALVALFGEVQVSVFKFNGSYDSPKVLFLIGLAATLAGPPLLYGILKSSKGQVGQDNPR
                     EQKE"
     gene            5140..5802
                     /locus_tag="VF240_22565"
     CDS             5140..5802
                     /locus_tag="VF240_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178058.1"
                     /translation="MSTFRMTRSRRVFLQAWAACGLLLASSGAVAQKQAPPDKNVLAK
                     GNLYYSSDDITDKAAREYREVKKKYKGTRDGEAAQYLLASYYHRKFYIVRAKQREDDE
                     DAIHEAQEEYEDYIKIYATAPSPEWLADSHFNLALIWFEYGHKAMALDYLNKLQQAAE
                     KDPKVYIYDVLWSPRSNDRVEGYYDSAQLAAFTREQINNGLTTGETVEALRAWCRGQQ
                     GE"
     gene            complement(5968..6954)
                     /locus_tag="VF240_22570"
     CDS             complement(5968..6954)
                     /locus_tag="VF240_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178059.1"
                     /translation="MTTTDTPRKMWAGAPSLIQIEACQQGGKRGAQLWGVQQNYKLNS
                     TFQVTPGGNWSEWIGPNWNGMNQQVFDIAGALQNNGNVQLWVLDNRQQLYSISQNSPG
                     GNWGQWSGPNWSGAPRMNAIGAAQQGGPRGAQLWGVTENQILTSTYQITPGGNWSPWQ
                     DWQKAPRCKDVAASGQNDGRLQLFVIDLELQLWIDWQTSPGGSWVGIQQPNWNNAPKM
                     WNVCAAQQGGPRGGQVWGITRDYKLVTCYQGSPGGNWSGWQDWANTPQVVEIAAAGQN
                     NGCVQLWAITSDGALISTKQTSPGGNWTGWETEVEIDFQGNPMTDNRRRDRP"
     gene            complement(7214..9316)
                     /gene="fusA"
                     /locus_tag="VF240_22575"
     CDS             complement(7214..9316)
                     /gene="fusA"
                     /locus_tag="VF240_22575"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7178060.1"
                     /translation="MKVYPTDHIRNLAVVGHGDAGKTQLLSSLLHVSGATPRWGKVDE
                     GTTVTDYEEDSIARKVTLNTALAHCEWRDHKINFIDTPGYAAFVTHARPALRVADCAL
                     VVVHAVKGIEVQTEKTWAYANEFLVPRMMVINQMDREHANFGMALDSARSTFSRAIVP
                     FTLPIGQEAGFRGVVDIVHMKAYEFKDGKAKEIDIPADGRDLADRERKRLIEVIAESD
                     DALMEKYFETDTLPEEDIVAGIPRAIAASRLCPVFAASSATLVGLQNVLDGIIDYAPA
                     PNAHEAEHGHTAAESDGGQPATRKYDAGEPFSAYVFRTVADPFAGRINVLKVVSGHVK
                     SDATVQNTSRGTPERLGALHSMQGKQLDKIEEANAGDIIACVKLKETQTGDTLSEKAA
                     PILYPSVEYPEAAIAFAIEPKSRQDEEKISAALHRILEEDPSLHFTRDAQTKEFILSG
                     SGQLHIETVVEKLRNRYHVEVALHPPKVPYKETITTRAEVQGRHKKQTGGRGQFGDCK
                     VVFEPLPRGGGFKFEDKIFGGSVPQQFRPAIEKGIVEAAAHGAIAGYPLVDFKVTLID
                     GSYHTVDSDEHSFRAAGRKAFRNAVEKVKPTLLEPIMNVEVVAPQEYSGDIMGDLNSR
                     RGRVQGMEMRGKNQVVKAQAPMSEMLDYQSKLNSITQARGSFHMEFSHYDPVPGQIAQ
                     KIVEQARSEGRIRAEEEE"
     gene            9724..10107
                     /locus_tag="VF240_22580"
     CDS             9724..10107
                     /locus_tag="VF240_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178061.1"
                     /translation="MRFGISTLKRTISALMIGAFLLIGMAAPAEAQRRGKNKNKWDRH
                     DTRGRHDNGRHTGWTRGRHLGWGNRDWDDDDRRRRRRRAFRRDRRDDRRDWRDNRRTS
                     QRRWDRNDWRTRAYGNRVGYNRRFR"
BASE COUNT         1817 a         3029 c         3379 g         1945 t
ORIGIN      
        1 cgacgatata gtgctgaggt aaagctcgtt cgtgaggata catagcgccc tacaatgcta
       61 agaggcaaaa caattgagcc gacttcggcg acccaactcc tattatgacg agtgggtcga
      121 tgttatgggc ggccgagccg agatcatgcg acttcgggtc ggccgccaca cttcgcgtca
      181 cgaactatcg ttaaatgatt agccgtcgtc gcagcccggt gcaagctcca actgccacgc
      241 tgcctatacg tgttgatcta ccagaatcgt gttcccgtcc gggtcggcaa tcatcaagct
      301 ggccgggcct gtggagcttt cgtccgcttc cgaaatcatg tttacgccac tcgctttgag
      361 ccggcgctgc aactctcgaa cgtccgtgaa ttctccgagt ggctgggcgt cgctatccca
      421 ccccgggttg aaggtgagaa tattcttatc gaacatgcct tgaaacaacc cgatagcgtg
      481 gtcgccgttt ttcataatca gccagttctg cgattgatct cccatgaaaa ctgtaaaacc
      541 caacttctcg taaaagacct tcgacgcctc gatatctttg acagccaagc tcacagaaaa
      601 agctcccagt tccataagtt cctcctaagt tttgtttccg gcgcaaaacg ccccaggctc
      661 agggaacgcc ttatatcaca accgcgtctc tggaacaaga cattgttgcg tacatgcaac
      721 accaagtgaa actcatcacg tcacgaattc agccgcgccg agccaacagc cttcaaggct
      781 aaccgccgga gcacaacgat gaatggaatg ctctcgaggc gtgggctgga atgagttgtt
      841 cggcggcgcg cccgcgcgag cctccggcct gtgaccgact actggtaaac ctcgccatcg
      901 tgtttgatcc agcccgctgc gtgacgcttg cctgggtcgc gatgtgtcca gtgaatcacg
      961 ccccctttgg cgttccattc atactcgccg ctgaacgaca ccacgtcgcc tttgcgaagt
     1021 ccgacaactc tcggcgccag atcgatgttg tgcgagatga ggagcgtctg gcccgaggcg
     1081 agcgccacga tgaagcgctg atgccggctg ccgtcgttgt cgtccgagag caccctttga
     1141 acgacgccct gcccttccac ctgaatgttg ctcgtgcgtt gctcgaaggc gcgggcgagc
     1201 gtctcgtcgc tgcccgagaa cgctcggggc ggcctgcgag gcttacgcgg gcgggatttc
     1261 tgtgcggctt cagccttgaa actatacgaa tcatcatgca gaggggcgct caagagaaga
     1321 cttaggccga agataagcga gatgagttta cgcgtatttt gtctcattat taccatccga
     1381 gttagtagcc gaactctaat gataccgaac ggttcggcct cacatctcca gcaagggagt
     1441 gaggccgaac cgttcggtct aaatgctttc ggacttcgcg gcgactgctt gccggccgag
     1501 ttagttcttc agcttctcaa gggcgctttt ggcgctcgcc agattcgggt caacctccag
     1561 agccttgctg tagaactcga cggccttatc cttctgacct ttgagcgcgt agaactcgcc
     1621 gatgctgtcg tagaggttgg cctctttggg gtacagctcg gcggcgagct tgaggagttc
     1681 gaggccggcg tcgcggcggc cgttggcggc gaggtcgtag gcgatggagt tgagggagcc
     1741 ggggccggcg acgccggtcg ggtcaatctc cttcgccttg agaagcaact cgcgcgcgcc
     1801 ggacggctcg gcgtaggcca gttgcgtgac ggcgaggtag gcgttggcgg tggctgagtt
     1861 ggggtagagt tcgcgtgcga ggcgtgcggc cttgaaggcc cgttcgcgtt cgcccgcgtg
     1921 gacgagctgg cgcacggccg gggcgaagtc gccgaggtag ttggggtaga tttcggcccc
     1981 cgcgaaggcg cgcttgaggg cgggcgcgag cgcgtcgaag tcggaggcgg acgacaccga
     2041 gtgacggcga acttcgtccg tgtagctgcg cgcccagccc cgcgaccact gctcgatcag
     2101 gcgctcgacc ggcgcggaga atttcagctc ggcggggtag tcggcgcgcg acttgtagag
     2161 ggcgatgtag tcggcgaaga aagagagcgc gcccgagccg gtgtaggcgt tgaggcgggc
     2221 ggggccgtgg cgctcggcga gacgcgcggc catcagcgag ccgaccttga cgaagggctg
     2281 gcccgccgcc ggatgccggc ccgcgccgag cttggccgcg gcctcctgcg cgttggcagc
     2341 gagggcgtcg gcgctcatgt gcgtgttgaa gtaggtgaac gcctcgcgag cctcggcgtc
     2401 ggtcttcgag agcggttggg ccgtgcgggc gaagtggagg agaccccact tgtatgtgtc
     2461 gtcgaggccg cggtagagcg cgtcctttgc gcggtcgccg gtgtaggcgg agacggccag
     2521 cggctcatct gtgagaatct ggaagagacg catgacgtag gcgtccgggt tggccgactc
     2581 gaaattgctc gcggcggcga gggcgaagtt ctgcgcgggg aagttgtaga gcgtggtgcg
     2641 cgtctcgttc tgcgcgccgc cgtgggcgac gaggaagcgg ccgttgagtg ggttggtctg
     2701 ccagccgagg ccgtagtccg tgagacgacc cgcggtggtg gccgcggtgt cgtacatccg
     2761 cgcgacgctc gccggcgcga ggagcttgcc cgtgttgaga gcgacggcga agcggagcag
     2821 gtcggggact gtggagcgcg tgccgccgcc ggcgaagcgg ctgctgatgt cgatgaactc
     2881 ggagttgcgg agctgtccgc cgacgagctg gtagccgcgc acgcggttgg ggatcagctc
     2941 cagcgggtcg tccatgcgcg tgtcgctcat cccggcgggc ttccagacgt tctcgcgcat
     3001 gtagtcggcg taggactgac ccgaagcggc ttcgatgatc gcgccgagga ggttgtagcc
     3061 gtaggacgaa tagctgtagc gcgtgcccgg ctcggcgacg aggtcgaagt tctcgaagat
     3121 ggcgatggcg gcgcgcgtgt ccttgtgctc cttgatgtgg ccctcggtgg cgaagtcgcg
     3181 gtagtggcta atgccgccga gatgcgcgag gagctgaccg atggtgacgg gccacttctt
     3241 cttcgggaag taggagacat gtttctgcac ctcgtcgtcg agattgagct tgccgcgttc
     3301 ggcgagctgc atgacggcga cggccgtcat cggcttggtg acggaggcga ggcggtaggc
     3361 cgactcggct cgcgccggaa gcttgttctc caaatccgcg tagccatagc ccctgaccca
     3421 gacgtagccg tctttgacga agccgatggt gagcgccgtc gtgcggtcgg ccgccatctg
     3481 ccggcggacg tactcgtcga actcgcgcag gcgctccgcg tacttcgccg gcaccgcctc
     3541 gtcgcgcgcc ttcggcgcga ccgtcgggtt tttggattgc gcgcgcgtcg ttgcggagta
     3601 cggcaggaag aaggaggagc ctgccagcga gacggacagg acgagtgcgg aaaatcttct
     3661 catgtggaac ctctctcgaa ggggtgtgtg cggccgatgc gccggggctg tggcacgaag
     3721 gatacaacga ctgcggccgc gggcggaagc cccggatttc tatttactct ttaccccccc
     3781 cgcggccggc tgtggtgaaa tgtttccact cggtcatgca taaccagaca gaggtgccag
     3841 atgaagtgcc cgtttccagg catctcgcct ggagcgtttg ggctttggcc ctggaaatac
     3901 cgcggcacac gcccatcccc attactcaca aagggggaaa tgaagtggac atcccacgta
     3961 aagcattcct gctgatggcc tggacgttgt tagcaacagc gggcgggccg gccgctcccc
     4021 tgagccgggg gctggagaat agtccccgcc ggtgcccgca ggacgcggag gaaatcagag
     4081 ttccctccgc cgcgtcgttg atctgtctgg agttcgacaa gaaggacgtt ctggtcggga
     4141 gcgtcaacat cccgttgacc gtacaaatca cttcactcaa agagaagccc ctcaaactcg
     4201 tggacttttc cccggccgat gacaacggca cggagaagct gatcaagttc gcgaagtcgg
     4261 gggaggagga acttccttta gagggtgggg tggaggtgag gagcgtgtat cgcttccact
     4321 tctccattgc cgagaacgcg gagccgcgga gctacgcgat ccgcgtccaa ctcaagtacg
     4381 cgagtaagga cccgccgccc tctgccgaat cgttccacct gaaggtggga ggctccggcg
     4441 cagtcagact cgccgacaac accaggacgg tttcggccat gaccggcgag atcactacgc
     4501 agcacctcga agtgaagggc gcgtactact acccggttcg cctccgccag gtgacgatct
     4561 actcggaccc cgcggacctc gtcgcccgag ttgccatccc tgcggacgtg cccatcagga
     4621 atcgcaaaga ggtggagacg atagacgtct ctttccccgt gcgtaaaatg gaattgaaag
     4681 attggatctc cgcgttcggc agtgagaata agctctggtt cgagttcgag tatgaggacg
     4741 acgagaagcg tgtgttgaaa agccgggacg cactcaagct ggccgtcagg cccagcgtcc
     4801 tggccctcgt ctcggcgatt ttactcggcg tgttggcggg cactatcgtg aagacagact
     4861 tgaagcgcct tcaggttgaa gggtacatca cgagaaggca gaaggctctc ttcatcgtcg
     4921 gcacgagcgt catcggcctc atcgcggcgt tggtcgccct cttcggcgag gtgcaggtca
     4981 gcgtcttcaa attcaacggc tcatacgaca gtccaaaggt gcttttcctg atcggcctgg
     5041 cggcgacgct ggccggcccg ccgctcctgt acggcatact caagtcgtcg aaaggtcagg
     5101 tggggcagga caacccgcgc gagcagaagg agtgaagctg tgtccacatt cagaatgact
     5161 cggtcgagaa gagtttttct acaggcgtgg gcagcctgcg ggctgctgtt ggcctcgtcc
     5221 ggggccgttg cccagaaaca ggcgccgccc gacaagaacg tgttggcaaa ggggaatctc
     5281 tactacagca gcgacgacat cacggataaa gccgccaggg aatacaggga ggtcaagaag
     5341 aagtacaaag ggacgagaga tggcgaagcg gcgcagtacc ttctggcgag ctattaccac
     5401 cgcaagtttt acatcgtcag ggcaaaacag cgcgaggacg acgaagacgc catccacgag
     5461 gcgcaggaag aatatgaaga ttacatcaag atttatgcta cggccccgtc gccggaatgg
     5521 ctggccgatt cgcatttcaa cctggcgcta atctggtttg agtatgggca caaggcaatg
     5581 gccctcgact accttaataa actccagcag gcggccgaga aagacccaaa ggtctatatc
     5641 tatgatgtcc tttggtcacc acgcagcaac gaccgggtgg agggctacta cgattccgct
     5701 caactggccg ccttcacacg cgagcagata aacaacggcc tcaccacggg tgagaccgtg
     5761 gaggcactaa gggcgtggtg ccggggtcag cagggcgaat aggagagtgc ccccaggacg
     5821 cgagctaagc gcagggccgt gggcgaatac cccacggccc tgcgcgcgag cggcaaattg
     5881 gagtccgagt ggtttactct tcttcctcgg ccctcggagc gggtcttctt tcgatgggct
     5941 ggcgcgtcgg ccgggcggct cccgcgctca tggcctgtca cgcctcctgt tgtccgtcat
     6001 cgggttgccc tgaaagtcga tctcgacctc ggtctcccac cccgtccagt tgccgccggg
     6061 cgaggtctgc tttgtgctga tgagtgcgcc gtcactggtg atggcccaga gctggacgca
     6121 gccgttattc tgtccggcgg cggcgatctc cacgacctgc ggcgtgttcg cccagtcctg
     6181 ccagccgctc cagttgccgc cgggcgagcc ctgatagcag gtgacgagct tgtaatcccg
     6241 cgtgatgccc cagacctgcc cgccgcgcgg gccgccctgc tgcgccgcgc agacgttcca
     6301 catcttcggg gcgttgttcc agttcggctg ctgaatcccg acccaactgc cgccgggcga
     6361 cgtctgccag tcgatccaga gttggagttc gaggtcgata acgaagagct ggaggcggcc
     6421 gtcgttctgg ccggaggcgg ccacgtcctt gcagcgcggg gctttctgcc agtcttgcca
     6481 cggactccaa ttgccgccgg gcgtgatctg gtaggtgctg gtgaggattt gattttcggt
     6541 gacgccccag agctgcgcgc cgcgcgggcc gccctgctgc gccgcgccga tggcgttcat
     6601 gcgtggcgcg cccgaccagt tggggccaga ccactgaccc cagttgccgc cgggcgagtt
     6661 ctggctgatc gagtagagct gctgccggtt gtccaacacc cagagctgga cgttgccgtt
     6721 gttctggagg gcgccggcga tgtcgaagac ctgctggttc atgccgttcc agttcggccc
     6781 aatccactcg ctccagttgc cgcccggcgt gacctggaag gtggagttga gcttgtagtt
     6841 ctgctggacg ccccagagct gtgcgccgcg cttgccgccc tgctggcacg cctcgatctg
     6901 gatgagtgag ggggctccgg cccacatttt tcggggggta tcggtagtcg tcatacgcct
     6961 gtctcctttg agggtaaggt aaggaacaga tgattcggcg aggttggggg tttcagtgtc
     7021 gcgtcagacg agggagtcgc gcagcagcga cgccgcgcga cggaaatatg cggagtgact
     7081 tgtgcgaccg cgctgatact aaccgcgcgc cgccgcgagg gtcaacagaa taaaaatcgg
     7141 gcggcgacgg acttcaccgt cgccgcccgc ccgtgcatat agcgcgagtc tgatgcacat
     7201 cccaagagat gcgttactcc tcctcctcag cacggatgcg gccctcggag cgggcctgct
     7261 cgacgatctt ctgggcgatc tgtccgggga cggggtcgta gtgggagaac tccatgtgga
     7321 aggagccgcg ggcctgtgtg atggagttga gcttcgactg atagtcgagc atctccgaca
     7381 tcggcgcctg cgccttgacg acctgattct tgccgcgcat ctccatgccc tgcacgcggc
     7441 cccgacggga gttgaggtcg cccatgatgt cgccgctgta ctcctgcggg gccaccacct
     7501 ccacgttcat gatcggctcc aaaagcgtcg gcttcacctt ctcgacggcg ttgcggaaag
     7561 ccttgcgccc cgccgcgcgg aaactgtgct cgtcggaatc gaccgtgtgg taggagccgt
     7621 cgatgagcgt caccttgaaa tcgacgagcg ggtagccggc aatcgcgccg tgcgccgccg
     7681 cctcgacgat gcccttctcg atggccgggc gaaactgctg cggcaccgag ccgccgaaga
     7741 ttttatcctc gaacttgaac ccgccgccgc gcggcaaagg ctcgaagacc accttgcagt
     7801 cgccgaactg accgcgcccg cccgtctgct tcttgtgtcg gccctgcacc tcggcccgcg
     7861 ttgtgatggt ctccttgtaa gggactttcg gcgggtggag cgccacctcg acgtgatagc
     7921 ggttgcgtag cttctcgacg acggtctcga tgtggagctg gcctgagccg gagaggatga
     7981 actccttggt ctgcgcgtcg cgcgtgaagt ggagcgaggg gtcttcttcg aggatgcggt
     8041 gcagagcggc gctgatcttc tcctcgtcct ggcgcgactt cggctcgatg gcgaaggcga
     8101 tggccgcctc cgggtactcc acgctcgggt agaggatggg cgcggccttc tccgagaggg
     8161 tgtcgcccgt ctgcgtctcc ttgagcttga cgcaggcgat gatgtcgccg gcgttggcct
     8221 cctcgatctt gtcgagctgc ttgccctgca tcgagtggag cgcgcccaat cgctcgggcg
     8281 tgccgcgcga agtgttctgc acggtggcgt cggacttgac gtggccgctg acgaccttga
     8341 ggacgttgat gcggccggcg aaggggtcgg ccacggtgcg gaagacgtag gccgagaaag
     8401 gctcgcccgc gtcgtacttg cgcgtggccg gctgaccgcc gtccgactcg gcggccgtgt
     8461 gcccgtgctc ggcctcgtgg gcgttgggcg cgggcgcgta gtcgatgatg ccgtcgagga
     8521 cgttttgcag accgacgagc gtcgccgaag aggccgcaaa caccgggcac agtcggctcg
     8581 ccgcaatcgc gcgggggatg cccgcgacga tgtcttcctc gggcagcgtg tcggtctcga
     8641 aatatttctc catcagcgcg tcgtccgact cggcgatgac ctcgatgaga cgcttgcgct
     8701 ctctgtccgc gaggtcgcgc ccgtcggccg ggatgtcgat ctctttggcc ttgccgtcct
     8761 tgaactcgta agccttcatg tggacgatgt ccacgacgcc gcgaaagccg gcctcctgtc
     8821 cgatgggaag cgtaaagggg acgatggcgc ggctgaaggt ggagcgggcg ctgtctaagg
     8881 ccatgccgaa attggcgtgc tcgcggtcca tctggttgat gaccatcatg cgcggcacca
     8941 gaaactcgtt ggcgtaggcc caggtcttct cagtctgcac ctcgatgccc ttgacggcgt
     9001 ggacgacgac gagcgcgcag tcggcgacac gcagggcggg ccgcgcgtgc gtgacgaagg
     9061 cggcgtagcc gggcgtgtcg atgaagttga tcttgtggtc gcgccactca cagtgggcga
     9121 gggccgtgtt taaggtcacc ttgcgcgcga tggagtcttc ctcgtagtcg gtgacggtcg
     9181 tgccctcatc gaccttgccc cagcgcgggg tggcgcccga gacgtggagc agcgagctga
     9241 ggagctgcgt cttgcccgcg tcgccgtggc cgacgacggc gaggttgcgg atgtggtctg
     9301 tcggatagac tttcatagaa tacggggaag cgtcctttcg agagttttag gggcgtgccg
     9361 cgcccgcctc gtcgccgagc ctgcacggtc gccctgccga agctgtatgc gggatgaaac
     9421 tgacttcggg ctgaggggct aatttatccg agcgtcaggc ggaaaagcaa gcggccaaaa
     9481 aaaccgtcaa tcgtcaatcg tcaatcgtca atcgcgaatc gaggttgaca ctacctcccc
     9541 cttgaaagtt tgccacccaa cgtcacggag actccgtcgc gatttacgat ttaccattga
     9601 cgattgacgg ccccggaggc atcatgtttg cacactctcc cggcagaacg gcggcgatgg
     9661 tcgccgtaaa acttcaacaa gtctctcccc ccgatgaact tacgttcttt caggaggtcg
     9721 ggtatgagat tcggaatcag tacactcaag cggacgatta gcgcgctgat gatcggggcc
     9781 ttcctgctca tcggcatggc cgcccccgcc gaggctcaga ggcggggcaa gaacaagaac
     9841 aagtgggaca ggcacgacac ccgaggccgc cacgacaacg gccgtcacac gggctggacg
     9901 cgcgggcgtc acctcggctg ggggaatcgc gactgggacg acgacgaccg ccgccgccgc
     9961 agacggcggg ccttcaggcg tgatcggcga gacgaccggc gcgactggcg ggacaaccga
    10021 cgcacgtccc aacggcgttg ggatcgcaac gactggagaa cacgcgccta cggcaatcgc
    10081 gtcggctaca accgccgctt ccgttaagac gcggcgcgtc gatggtcaac caatctgtcc
    10141 gagcaagaga agggcgcggc ccgatagatc
//
LOCUS       DASVES010000531        11847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_517313, whole genome shotgun sequence.
ACCESSION   DASVES010000531 DASVES010000000
VERSION     DASVES010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517313"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..3137)
                     /locus_tag="VF240_22585"
     CDS             complement(99..3137)
                     /locus_tag="VF240_22585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7178062.1"
                     /translation="MDFIRRNLIRTTALILALAAGALSASAQQSGAGSIRGQVMDELG
                     GVVVGVSVTAVIPGAQERAAVTDAEGNYQIAGLPAGKYTVRATGAGFSLYENAEVEVA
                     AGARQQLNITLGVALERDEVTVTADPGVSTDADANADALVLRDKDLDALPEDPDELAA
                     ALQALAGPSGGPNGGQIFVDGFSGGRMPSRDTIREVRINQNPFTAENDRPGFGGRIEV
                     LTRPGTDRLRGTSYFTFMDESLNSRNPFLRSDKRPPFQIRRYGGNLSGPIKPKVASFF
                     VDLERNEDDENDIINARVLDPITLEPTPFTLGVLTPNRRTSFSPRFDYQINAQNTLVG
                     RYQFFRQQFQNSGLGSEFTLPTRAFDSTTQQHQLQLTETAVINQRVINETRFQFTRNR
                     NERDGDNTIPAVNVQGAFNGGGSQVGQSFSEDTRFELQNNTTWTLGSHAFKAGARLRG
                     VSIKDVSVQNFGGTFVFSSLDQFRSNVAGLGVGPTQFTIASGEPEADVRQYDFGGFIQ
                     DDWRVRPNLTLSAGLRYERQSNIDSNLNFAPRVAIAWQPGTQQQGQPARTVVRAGFGV
                     FYDRVNENLTLQENRFNGSQQQFIVDQRTEAGIDFLNQVRLNADGSVAFLPALSGLSA
                     VPQITRRLDDRIESPHTYFGGLNVERQLTKTFTAFVSVGTIRSRHLLRLRNINAPLPG
                     TFGVAGIDPVRPFPGAGDILQYESSGVSNMNQFSVGFNTRLNPNFSIFANYTLASAKS
                     DTDGGFGGPFGGGGIASPANSYDFSNEYGRSSFDMRHRLFVHGSFTVTKWRIQLNPFI
                     QANSGRPFNIITGQDTNGDTLFTERPSLVSGDTCATRTALSPNVVCTRFGTFNLRPLA
                     GEQTIQRNYGQGPNFFVVNLRIGKTFGFGDPPGAARETAANATQQPQGSGGQRGGSGG
                     RGNSGGGGGARPAPSGPTMGGGMFMMMGGAPTEKRYNINLSINIQNIFNRANFSQPVG
                     NLLSPDFGNSIALAQSFGFGGGGSAAAGNRKIQAQIRFSF"
     gene            complement(3291..4532)
                     /locus_tag="VF240_22590"
     CDS             complement(3291..4532)
                     /locus_tag="VF240_22590"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX7178063.1"
                     /translation="MKKLCIVSALVLFALSLSANAQTSAPHAPPTAPQKSDPGITPNL
                     VLGDVVATDTAGKQIVLKTDAGSVVIVTVADNTIIRKNPPGETKLDKAVPMTFAEIGV
                     GDRVLARGKPADDGKSIPARALIVNTKAELTAKQEAERAEWKRRGVVGTVTAVNAAAG
                     EITLTTGRTPEGPQTAVVVAGQATKFRRYAPDSVKFADAKSSTFEELKAGDQLRAKGE
                     KSEDGKRLTAEEIVSGSFRTILGTVTAVDAAKNEITVKSSQGGQDVSVVISRDSMLKR
                     FPDMGAMGGGGMVRMQAGPGGAPAGGGAQHGGARPEGATQGGGPQRRMMGGPDAMQEM
                     LERLPATTVAEVKPGQMIVFSTTGVDPTRVTAIQLIAGVEPLVAMMQARGGRVSGGGP
                     GGAGMGGGDGGMGFGFGIGQP"
     gene            complement(4993..6600)
                     /locus_tag="VF240_22595"
     CDS             complement(4993..6600)
                     /locus_tag="VF240_22595"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7178064.1"
                     /translation="MSRKHSLFSLLFLAIAIAAPLAVAAQRRPGTATPGARPAQAKTP
                     APTPTPGRAATTPAPAAAVVAPVVQDECGCEPKLLPEVLAVANGVRITKADISPAAQA
                     QIDKLKGQVIEARAGELDLQINSRLLDAEAKKRGTTSAKLLEQEVIAKTTDPTDAEAQ
                     VFYDQNKARINEEFAKVRADIIGYLREQRQREAAKALADRLRAASDVKVLVQKPKPPA
                     TDAERARVLATVNGQNITSSDIEDSLKPLVFEAQEKIFQLRKREVDIKINDLLLEQEA
                     QKRQVTARALLETEVKSKLTPVTEADAQKFYNENKTRINGDFAQTKSDIIQFLQQQAE
                     GNLTSAFAQRLRNAASVQTFITQPEAPVYQIATDDQPSKGAATAPVTVVEFTDYQCPS
                     CAQTHPVIERLAAEYGDRLRVVARDFPLSMHENAFKAAEAAEAAREQGKYWEYVALLY
                     RNQSALGAQNLREFALAAGLDVTRFDAALSSGKLAEKVQRDLIDGQRLGVGSTPTIFV
                     NGRRVNDRSYEGLKLRIEEALKTAPKK"
     gene            complement(6832..7803)
                     /locus_tag="VF240_22600"
     CDS             complement(6832..7803)
                     /locus_tag="VF240_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7178065.1"
                     /translation="MSNAAAAFLLLLLAAVAASAQGEAPAPPPQSAPAQTVSQALHEA
                     LANRTNEMVVEGEQAGDVFGVGRSVRVRGTVKQGVMAFGGDVVVEGRVEGDVATVGGS
                     VSQAEGSYIGGDVMVLGGAYHHGKAAPGRNPASKTIMFAGYEQELRALVHDPAQLLRP
                     NFSLGFFGLRLLAILFWFVVSLALTAVTPGSVGRAAARLQLTSLRVALIGLLGALVLV
                     PGVIVSLHFLPPVLGLLVGFTALILLLLSYLFGRVVIHAATGRWLQRSLLREESRSES
                     VALLLGAAFWAVALVLPYVWPVVVAGLVVTSLGLALTARYRVSWKRD"
     gene            complement(7905..8504)
                     /locus_tag="VF240_22605"
     CDS             complement(7905..8504)
                     /locus_tag="VF240_22605"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7178066.1"
                     /translation="MSDVNPKVAANCPRVESVAAYLDGEMDAPPAALFESHVKECQSC
                     AGALREQRRLLCLLDNAFGAHAGDAERPVELPADFARVVAAHARTDMCGVRSRAERAF
                     SVKLSLALAALTALLLGASAGDSVLGPAAAVARTAAGVAGMLGGALLDAVAAVVVILR
                     ALGGRLVAGPPPVAALYCLLFAGASLLLLRLIKGYHRAR"
     gene            complement(8523..9140)
                     /locus_tag="VF240_22610"
     CDS             complement(8523..9140)
                     /locus_tag="VF240_22610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7178067.1"
                     /translation="MRMNTAAATAGFNPSATADSDLVVRAIAGREESFEELVRRYQRP
                     IAAYVYRMVGDYDSALDLTQEVFIKVYNSLARYRAEYKFSTWIYRIAHNSAIDHLRRH
                     NMRFEDLERSGEDGTSYEKPLASSAPTPEQMSERSERRAEIEGVVQNLPPAYRELITL
                     RHALDFSYDEIAEVTGLPLGTVKNRIFRAREAMREELSRRGITSV"
     gene            9239..9454
                     /locus_tag="VF240_22615"
     CDS             9239..9454
                     /locus_tag="VF240_22615"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX7178068.1"
                     /translation="MKDKSIITSSADVMGGAPVFTGTRVPVQTLLDYLKGGESIDDFL
                     EGFPTVAREQVIALLEEAEEQIIKLVA"
     gene            9457..9774
                     /locus_tag="VF240_22620"
     CDS             9457..9774
                     /locus_tag="VF240_22620"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX7178069.1"
                     /translation="MKILLDECIDRRLARELGGHEVKTVPQMGWAAKKNGELLMLARQ
                     QFEVFITVDRNLAFQQSLPDFDIAVLILRASSNRLAELKLLAPKILAILPILTKGKAE
                     SVS"
     gene            complement(9785..10786)
                     /locus_tag="VF240_22625"
     CDS             complement(9785..10786)
                     /locus_tag="VF240_22625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178070.1"
                     /translation="MSTLRKLFEPRLPSEAIGLSGEGACVVSLDHRRGDVFNVRKAAY
                     AALREGLVRPSFDEQNISDTAELADALAELVTNVGLLKRRRWSVALPEAAARTSILTL
                     EGTPSSRGEREEMLRWKTERTVGASLDELRVGRERLRPDSQGRDRYLLTAVRLSVLSE
                     YERVFEALGWQTGLMLPRHAGEAWWLMRRGGAAAAGDSLLVSSHREGFTAGVFRAGQP
                     MLLRSVICDPEDRADELYRFVLFYRDRNAPPPGEPEAANAVASAAPAAAPDETIETML
                     VAGTGLDAAEAGAIVRETLSVPPRALGPGDLRLSFPSGQLDFNLIAAPAGLAALAWA"
     gene            complement(10961..11464)
                     /locus_tag="VF240_22630"
     CDS             complement(10961..11464)
                     /locus_tag="VF240_22630"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7178071.1"
                     /translation="MTGKGKRLKVKGEGGRGPLERDRRSALTPIPLPLPRRAGRGFTL
                     LELMIVISVIIILALIVMPLYNRTVQAAKEAVLRENLQQMRKMIDQYAADKGKLPSGG
                     QDLVTAGYLHSIPDDPMTETEGDDWNWVMGDDPNLGEGGQGLVNVCSSSGDNDTQGVS
                     YNNCDKW"
     gene            complement(11454..>11847)
                     /locus_tag="VF240_22635"
     CDS             complement(11454..>11847)
                     /locus_tag="VF240_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178072.1"
                     /translation="QLYLDPRSKVAISDRTIFDTSNLDHYPPTLKILVEGVNVVPRLN
                     LQGSSPDINRPIESGGTQLSLRKKIYLRRIPDDPMTDEDESSGEAEWGTRSCYDEGDS
                     SSWDGINVFDVYSKSDGTALNGEKYSDW"
BASE COUNT         1847 a         3770 c         4168 g         2062 t
ORIGIN      
        1 aaaagggcgg gccgcgttga ccatgatcaa cgcggcccgc cctgatgact gacggttctg
       61 tctcaagcgg cgagcttcaa cgcgactgcg gcaggctttt agaagctgaa gcgaatctgc
      121 gcctggattt tgcggttgcc ggccgccgcg cttccgccgc cgccgaagcc gaagctctga
      181 gcgagggcaa tcgagttgcc gaagtcgggc gacagaagat tgccgacggg ctgcgagaag
      241 ttcgcgcgat tgaagatgtt ctggatgttg atcgacaggt tgatgttgta gcgcttctcg
      301 gtcggcgcgc cgcccatcat cataaacatc ccgccgccca tcgtggggcc gcttggcgcg
      361 gggcgagcgc caccgccacc gcctgagtta ccgcggccac cgctgccgcc gcgctgtccg
      421 ccgctacctt gcggttgctg cgtcgcgttt gccgccgtct cgcgcgccgc gcccggcggg
      481 tcgccgaagc caaacgtctt gccgatgcgg aggttgacga cgaagaagtt cggcccctga
      541 ccgtagttgc gctggatggt ctgctcgccc gcgagcgggc gcaggttgaa ggtgccgaag
      601 cgcgtgcaga cgacgttcgg cgacagcgcc gtccgcgtgg cgcaggtgtc gccggagacg
      661 agcgacggcc gctcggtgaa gagcgtgtcg ccgttcgtgt cctgccccgt gatgatgttg
      721 aaggggcggc ccgagttggc ctggatgaac gggttgagct gaatcctcca tttcgtcacg
      781 gtgaaagagc cgtggacgaa caggcggtgg cgcatgtcga agctggagcg cccgtactcg
      841 ttcgagaagt cataagagtt ggcgggcgag gcgatgccgc cgccgccgaa ggggccgccg
      901 aagccgccgt cggtgtccga cttcgcgctc gccagcgtgt agttggcgaa gatggagaag
      961 ttcgggttga gccgcgtgtt gaagccgacg gagaactggt tcatgttcga gacgccggac
     1021 gactcgtact ggaggatgtc gcccgcgccg gggaaggggc gcacggggtc gatgcccgcc
     1081 acgccgaacg tgcccggcag cggcgcgttg atgttacgca gccgcagcag gtgacgcgag
     1141 cggatggtgc cgacggagac gaaggcggtg aaggttttgg tgagttgccg ctcgacgttc
     1201 aagccgccga agtaggtgtg cggcgattct atccggtcgt cgaggcggcg cgtgatctgc
     1261 ggcacggccg aaaggccgga gagcgccggc aggaaggcga cgctgccgtc cgcattgaga
     1321 cgcacctgat tgaggaagtc gatgccggcc tcggtgcgct gatcgacgat gaactgctgc
     1381 tggctgccgt tgaagcggtt ctcctgaagc gtcaggtttt cgttgacgcg gtcgtagaag
     1441 acgccgaagc cggcgcggac aaccgtccgg gcgggctgcc cctgctgctg cgtgcccggc
     1501 tgccacgcga tggcgacgcg cggcgcgaag ttgagattgc tgtcgatgtt cgactgccgc
     1561 tcgtagcgaa ggcccgccga gagcgtcagg ttgggacgca cccgccagtc gtcctgaatg
     1621 aagccgccga agtcgtactg gcgcacgtcg gcctcaggct cgcccgacgc gatggtgaac
     1681 tgcgtggggc cgacgcccaa gccggcgacg ttcgagcgga actggtcgag cgagctgaag
     1741 acgaaggtgc cgccgaagtt ctggacggaa acgtctttga tggagacgcc gcgcaggcga
     1801 gcgccggctt tgaaggcgtg cgagccgagc gtccacgtcg tgttgttctg aagctcgaag
     1861 cgcgtgtcct cgctaaacga ctgtccgacc tgcgagccgc cgccgttgaa ggcgccctgc
     1921 acgttgacgg ccgggatggt gttgtcgccg tcgcgctcgt tgcggttgcg cgtgaactgg
     1981 aagcgcgtct cgttgatgac gcgctggttg atgacggccg tctcggtgag ctgtaattgg
     2041 tgctgctgcg tcgtcgagtc gaacgcgcgc gtgggcaacg tgaattcgct gccgaggccg
     2101 gagttctgga actgttgccg gaagaactgg tagcggccga cgagcgtgtt ctgcgcattt
     2161 atctgatagt caaagcgcgg gctaaaggaa gtccggcggt tcggcgtcag gacgccgagc
     2221 gtgaacggcg tcggctccag cgtgatgggg tcgagcacgc gcgcgttgat gatgtcgttt
     2281 tcgtcgtcct cgttgcgctc cagatcaacg aagaaggagg cgaccttcgg cttgatgggg
     2341 ccggagaggt tgccgccgta gcggcgaatc tggaaaggcg ggcgcttgtc ggagcgcagg
     2401 aacgggttgc gagagttgag gctctcgtcc atgaaggtga agtaggaggt gccgcgcagg
     2461 cggtcggtgc cggggcgcgt gagcacctcg atgcgcccgc cgaagccggg gcggtcgttc
     2521 tcggcggtga acgggttctg attgatacgc acctcgcgga tggtgtcgcg cgagggcatc
     2581 cggccgccag agaaaccatc gacaaaaatc tgtccgccgt tggggccgcc cgaggggccg
     2641 gcgagcgcct gaagggcggc ggccagctcg tcggggtctt cgggcagggc gtcgaggtct
     2701 ttgtctcgga ggacgagcgc gtcggcgttg gcgtccgcat cggtgctgac gccggggtcg
     2761 gccgtcaccg tcacctcgtc gcgttcaagg gccacgccga gcgtgatgtt gagctgctgg
     2821 cgcgcgccgg cggcgacctc gacttcggcg ttctcgtaaa gcgagaagcc cgcgcccgtg
     2881 gcgcgcacgg tgtacttgcc cgccggcaga cccgcgatct ggtaattgcc ctcggcgtcg
     2941 gtgacggccg cgcgctcctg agcgccgggg atgacggccg tgacgctcac gccgacgacg
     3001 acgccgccga gttcgtccat cacctgcccg cggatcgagc cggcgccgga ctgctgcgcc
     3061 gacgccgaca gcgcgccggc cgcaagcgcg agaatgagcg ccgtggttcg tatcaggttt
     3121 ctgcgtatga agtccatttg attgcctact ctgaattaga atttattgac tcgttggaac
     3181 tgacttacgg atagtaccgc gagcggtagc gagcgggtgg cgctcaaatc agtggtgcgc
     3241 ctcgggtgcg ttgacccgct cgctaccgct cgcggtactg ttgtcatgct ttacggctgg
     3301 ccgatgccga agccgaagcc catgccgccg tcgccgccgc ccatacccgc gcctccgggg
     3361 ccgccgccgc tcacacgccc gccgcgcgcc tgcatcatcg ccaccagcgg ctcgacgccc
     3421 gcgatgagtt gaatcgccgt tacccgcgtg ggatcgacgc ccgtcgtgct gaagacgatc
     3481 atctggccgg gcttgacctc agcgacggtc gtcgccggca gacgctcaag catctcctgc
     3541 atcgcatctg ggccgcccat catgcggcgc tgcgggccgc caccctgcgt cgccccctcg
     3601 gggcgcgctc cgccatgctg agcgccacct cccgcagggg cgccgcccgg cccggcctgc
     3661 atgcgtacca tgccgccgcc gcccatcgcc cccatgtcgg ggaaacgctt gagcatcgaa
     3721 tcgcggctga tgacgacgga cacgtcctgc ccgccctgac tgctcttgac cgtgatctcg
     3781 ttcttcgccg catcgaccgc cgtcaccgta ccgagaatcg tgcggaacga gccggagacg
     3841 atctcttcgg cggtgaggcg cttgccgtct tcactcttct cgcccttcgc gcgcagttgg
     3901 tcgccagcct tcagctcctc gaaggtggac gacttggcgt cggcgaactt cacggagtcg
     3961 ggcgcgtagc ggcgaaactt cgtcgcctgc cccgccacga cgactgccgt ctgcggccct
     4021 tcgggcgtgc gcccggtcgt cagcgtgatc tcgccggccg ccgcgttaac ggccgtgacg
     4081 gtgccgacca cgccgcggcg cttccactcg gcgcgctcgg cctcctgctt ggccgtcagc
     4141 tcggccttcg tgttgacgat gagcgcgcgc gccgggatgc tcttgccgtc gtcggccggc
     4201 ttgccgcgtg ccagtacacg gtcgccgacg ccgatctcgg cgaaggtcat cggcacggcc
     4261 ttgtcgagct tcgtttcgcc cggagggttc ttgcggatga tggtgttgtc ggcgaccgtg
     4321 acgataacga ccgagccggc gtccgtcttg agcacgatct gcttgccggc cgtatcggtc
     4381 gccaccacgt cgccgaggac gagattgggc gtgatgccgg ggtcagactt ttgcggggcc
     4441 gtgggcggcg cgtgcggcgc cgaggtctgc gcgttggcgc tcaggctcag ggcaaaaagg
     4501 acgagggccg aaacaataca cagtttcttc atcaaataat ccttccggtc ggccggcgct
     4561 gtttttggtt gctgacgggt cggggcgaat tgcccgactc gttcgggtta accacctgtc
     4621 ttacgtcgcg ccgggcgggt tagttctcgc ctatcccagt cgcaagttcc ctgccacgcg
     4681 gcatttacca ataatttaca actgccccca gccccgcgcc gcctccggcc cctcgacacg
     4741 tccctgcctt ttgaatttca gcgaatgtgg tcgaaaggtt cccgaaactg cggggaatgc
     4801 tcgcacctgc ttagacgcct tacccccccg gtcggttaca ggcaaatccg tgccggcgaa
     4861 aaaaaatcgg cccggcgctc gggggccggg cctgttgggg gtcgagaact gcgagccgcg
     4921 cgcgagtgtg tccgcacgac tttcacgcgg gcgaccgggc atgttccacg cgcgccgcgc
     4981 ggacggtctc gcctacttct tcggcgcggt tttgagcgcc tcttcgatgc ggagcttcag
     5041 cccctcgtaa gagcggtcgt tgacgcggcg gccgttgacg aagatggtgg gcgtggagcc
     5101 gacgccgagt ctctgcccgt cgatgagatc gcgctgcacc ttctcggcca gcttgccgga
     5161 gctgagcgcg gcgtcgaagc gcgtcacgtc gaggccggcg gcgagcgcga actcccgcag
     5221 gttctgcgct ccgagcgccg actggttgcg gtagagcagc gcgacgtact cccagtattt
     5281 gccctgctcg cgggcggcct cggcggcctc ggcggccttg aaggcgttct cgtgcatgga
     5341 gagcgggaag tcgcgggcga cgacgcggag gcggtcgccg tactccgcgg cgaggcgctc
     5401 gatgacggga tgcgtctgcg cgcagctcgg gcactggtag tcggtgaact cgacgacggt
     5461 gacgggggcc gtcgccgcgc ccttcgaggg ctggtcgtcg gtggcgatct ggtagacggg
     5521 cgcttcgggc tgcgtgatga aggtctggac ggaagcggcg ttgcgcaggc gctgcgcgaa
     5581 ggccgatgtc aggttcccct cggcctgctg ctggaggaac tggatgatgt cactcttggt
     5641 ctgcgcgaag tcgccgttga tgcgcgtctt gttctcgttg tagaacttct gcgcgtcggc
     5701 ctcggtgacg ggcgtcagct tcgacttgac ctccgtttcg agcagagcgc gcgccgtgac
     5761 ctgtcgcttc tgcgcctcct gctccagcag caggtcgttg atcttaatgt ccacctcgcg
     5821 cttgcgcagt tgaaagattt tctcctgcgc ctcgaagacg agcggcttga gagagtcttc
     5881 gatgtcgctc gaagtgatgt tctggccatt gaccgtggcg aggacgcgcg cgcgctcggc
     5941 atccgtagcg ggcggcttcg gcttctggac gagcaccttc acgtcggagg cggcgcgcag
     6001 acggtcggcc agcgccttcg ccgcctcgcg ctggcgctgc tcgcgcaggt agccgatgat
     6061 gtcggccctg accttcgcga actcttcgtt gatgcgcgcc ttgttctggt cgtagaagac
     6121 ctgtgcctcg gcgtcggtcg ggtcggtcgt cttcgcgatg acctcttgtt cgagaagttt
     6181 ggcggaggtc gtgccgcgct tcttggcctc ggcgtcgagg aggcgcgagt tgatctgcaa
     6241 gtccaactcc ccggcgcgcg cttcgatgac ctgccccttg agcttgtcga tctgggcctg
     6301 cgcggcggga ctgatgtcgg ccttcgtgat ccgcacgccg ttggcgacgg ccagcacttc
     6361 cggcaggagc tttggctcgc agccgcactc gtcttgcacc accggcgcga caactgccgc
     6421 ggcaggggca ggcgtggtgg cggcgcgccc cggcgtgggc gtcggcgcgg gcgtcttagc
     6481 ctgagcgggg cgcgcgcccg gcgtcgccgt tccgggccgc ctctgcgccg cgacggcgag
     6541 gggcgcggcg atggcgattg ccaggaacag aagtgagaac aggctgtgct tccgactcat
     6601 tgaatttctc tccgatcttt ctccgaactg aggttcgccc cggccgcatc cggtggcgcg
     6661 tcagcgagcg cgaggcagac cacgaccgcg cgggagcttg agactcctca tcaattcgag
     6721 gccccttcaa acgggacgcg ggccggcggg aagacgttcg cggtcagggg attttagcat
     6781 ccggcagctt tggttggcga ctctctcggc cgtcgcccgc gcgggcgcga ctcagtcgcg
     6841 cttccagctc acgcggtagc gcgccgtcag cgcgagtccg aggctcgtca cgaccagtcc
     6901 ggcgacgacc acgggccaca cgtagggcag caccagcgcg accgcccaga aggccgcgcc
     6961 gaggagcagc gccaccgact ccgaacggct ttcctcgcgc agcagcgagc gctggagcca
     7021 gcggcccgtc gccgcgtgga tgaccacccg gccgaagagg taggagagca gcaggaggat
     7081 gagcgccgtg aagccgacga gcagcccgag cacggggggc aggaagtgca gggagacgat
     7141 gacgcccggc accagcacga gcgcgccgag cagaccgatg agggcgacgc gcagcgaggt
     7201 cagttgcaga cgcgcggcgg cgcggccgac cgagccgggc gtgacggctg tgagcgccag
     7261 cgagacgacg aaccagaaga ggatggcaag gagtcgcagc ccgaagaagc cgagcgagaa
     7321 gttcggacgc aggagttgcg ccgggtcgtg aaccagagcg cgcagctcct gttcgtagcc
     7381 ggcgaacatg atggtcttac tggcggggtt gcggcccggc gcggccttgc cgtggtgata
     7441 cgcgccgccc agcaccatca cgtcgccgcc gatgtaggag ccttcggcct gcgagaccga
     7501 cccgccgacg gtcgccacgt cgccctcgac cctcccctcg accacgacat cgccgccgaa
     7561 ggccatcacg ccctgcttga ccgtgccgcg cacacgcacc gagcggccga cgccgaacac
     7621 gtcgcccgcc tgctcgccct cgacgaccat ctcgttcgtt ctgttagcga gagcctcgtg
     7681 gagggcttgc gagaccgtct gcgccggggc ggactgcggc ggcggcgccg gcgcttcgcc
     7741 ctgcgcggag gcggcgacgg cggcgaggag tagcagcagg aaggcggccg ccgcattact
     7801 caacgaggcg tgcgcgcccg accggcgagc gcgcggccgt gtgcctctga tgataatcgc
     7861 gtcggcggcc attgttttgt ctgcgggagg agggtcgggg gttttcagcg cgcgcggtgg
     7921 tatcccttga tgagtcgcag cagcaacagg gacgcgcccg cgaagaggag acaatagagg
     7981 gcggccacgg gcggcgggcc ggcgacgagc cggccgccga gcgcgcgcag gatgacgacg
     8041 acggccgcaa cggcgtcgag gagcgcgccc ccgagcatcc cggccacgcc ggccgccgtg
     8101 cgcgcgacgg ccgcggcggg gccgagcacc gagtcgccgg cggaggcgcc gagcagcagg
     8161 gccgtcagcg cggccagcgc gagcgagagc ttgacggaga aggcgcgctc cgcacgggag
     8221 cggacgccgc acatgtccgt gcgcgcgtgg gccgcgacga cgcgggcgaa gtccgcgggc
     8281 agctcgacgg gtcgctcggc gtcgcccgcg tgcgcgccga aggcgttgtc gagcagacac
     8341 aaaagccggc gctgctcgcg gagcgcgccg gcacacgact gacattcttt gacgtgcgac
     8401 tcgaagaggg cggccggcgg cgcgtccatc tcgccgtcca gataggcggc gacggattcg
     8461 acgcgcgggc agttggccgc aaccttcggg ttcacgtcag acactcctct tcccttcctc
     8521 acttacacgc tcgtgatgcc tcgcctcgac aactcctcgc gcatcgcctc gcgcgcgcgg
     8581 aagatgcggt tcttgaccgt gccaaggggg agtcccgtca cctcggcaat ctcgtcgtag
     8641 ctgaagtcga gcgcgtggcg gagcgtgatc agctcgcggt aggcgggcgg caggttctgc
     8701 accacgccct cgatctccgc gcgccgctcg gagcgctcgc tcatctgctc cggcgtcggg
     8761 gccgaagagg ccagcggctt ctcgtagctc gtcccgtctt cgccgctgcg ctcgaggtct
     8821 tcaaagcgca tgttgtggcg gcgcaggtgg tcgatagccg agttgtgggc gatgcggtaa
     8881 atccaggtcg agaacttgta ctcggcgcgg tagcgcgcga gcgagttgta gaccttgatg
     8941 aacacttcct gcgtcaggtc gagcgccgag tcgtagtcgc caaccatgcg atagacgtag
     9001 gcggcgatgg ggcgctgata gcgacgcacc agttcctcga agctctcctc gcggcccgcg
     9061 atggcgcgca ctacgaggtc gctgtcggca gtggcagagg ggttaaagcc cgcggtggcg
     9121 gcggccgtat tcatacgcat gatacaaagc gtttacgcgc gggcgggagt gaaagtttca
     9181 aggctacttc cgcaagtttc gcttgatttc cgcaggcccg tttaactacc atcggcccat
     9241 gaaggataag tcaatcatca cctcgtcggc cgacgtgatg ggcggagccc cggtcttcac
     9301 agggacgcgg gttcccgtcc agaccctcct cgattacctc aagggcggag agtccatcga
     9361 cgactttctg gaggggttcc ccaccgtcgc aagggagcag gtcatcgcgc tcctcgaaga
     9421 ggccgaagag cagatcatca agctggtagc ttgagcgtga agattctcct ggatgaatgc
     9481 atcgaccgcc gcctcgcccg agagctgggc gggcacgagg tcaagaccgt cccgcagatg
     9541 gggtgggcgg cgaaaaagaa tggtgaactc ctcatgctcg ctcggcaaca gtttgaagtc
     9601 ttcatcactg ttgaccgcaa tctggccttc caacaaagcc ttcctgattt cgacatcgcc
     9661 gttttgatct tgcgcgcgtc gtctaaccga ctcgccgaac tgaagcttct cgcgccaaaa
     9721 atacttgcca tcctccccat actaaccaaa ggcaaggccg agtctgtgag ctaataactt
     9781 aagctcacgc ccacgccagc gccgcgaggc cggccggggc ggcgatgagg ttgaagtcga
     9841 gctggcccga ggggaaggag aggcggaggt cgccggggcc gagggcgcgc ggcgggacgc
     9901 tcagggtctc gcggacgatg gcgcccgcct cggcggcgtc gagtcccgtg ccggcgacga
     9961 gcatggtctc gatggtctcg tcgggcgcgg cggccggggc ggccgatgcc acggcgttgg
    10021 cggcttccgg ttcgccgggg ggcggcgcgt tgcggtcgcg atagaagagg acgaaacggt
    10081 agagttcgtc ggcgcggtct tcggggtcgc agatgacgct ccgcagcagc atgggctggc
    10141 ccgcgcggaa gacgccggcc gtgaacccct cgcggtgcga gctgacgagt agagagtcgc
    10201 ccgcggcggc agccccgccg cgacgcatca gccaccacgc ctcgccggcg tggcgcggca
    10261 gcatcagccc cgtctgccag ccgagcgcct cgaagacgcg ttcatattcg gacagcaccg
    10321 acagccgcac ggccgtgagc aggtagcggt cgcgcccctg cgagtcgggc cgcaggcgct
    10381 cgcgccccac gcgcagttcg tcgagagagg cgccgacggt gcgctcggtc ttccagcgca
    10441 gcatctcttc gcgctcgccg cgcgaggagg gcgtgccctc cagcgtcagg atcgacgtgc
    10501 gcgccgcggc ctcggggagc gccacggacc agcggcggcg cttcaacaaa ccgacgttcg
    10561 tcaccagctc ggccagcgcg tcggccagct ccgcggtgtc cgagatgttc tgctcgtcga
    10621 agctcggacg cacgaggccc tcgcgcaacg cggcgtaggc ggccttgcgg acgttgaaca
    10681 cgtcgccgcg ccggtggtcg agcgagacga cgcacgcgcc ctcgcccgac aggccgatag
    10741 cctcggaggg cagtcgcggc tcgaacagtt ttctgagtgt gctcatgaag aagctgttag
    10801 ctataagctg ttagccttta gctagcagct tttgtcagtg ggcagtggtt agttgtttgt
    10861 gtgtggcaac tggatgctga cattaataag gcccaagcgg ggcgacggaa acaacgagcg
    10921 actgaccacg gacagctttt agctaattgc taacagctcc tcaccacttg tcgcagttgt
    10981 tgtaggagac gccctgagta tcgttgtcgc cggaggagct acagacgttg acgaggccct
    11041 gcccgccttc gccgaggttg gggtcgtcgc ccatcaccca gttccagtcg tcgccttccg
    11101 tctccgtcat cggatcgtcg gggatggagt ggaggtagcc ggccgtcaca aggtcttgcc
    11161 cgccggaggg cagcttgccc ttatcggccg cgtactggtc gatcatctta cgcatctgct
    11221 gcaagttctc gcgcagcacc gcctccttcg ccgcctgcac cgtccggttg tagagcggca
    11281 tcacgatgag cgccaggatg atgatgaccg agatgacgat catcaactcc agcagagtga
    11341 agccgcgacc ggcgcggcgg ggaaggggta aggggatagg ggtaagggcg gagcggcggt
    11401 cgcgttcgag cggccccctt cccccttccc ctttaacctt taaccttttg cctttaccag
    11461 tcactgtact tctccccgtt cagggccgtg ccatcggact tggagtaaac gtcgaagacg
    11521 ttgatgccgt cccagctcga tgagtcgccc tcgtcgtagc aggagcgcgt gccccattcg
    11581 gcctcgcccg aggattcgtc ttcgtccgtc atcgggtcgt cggggatgcg ccgcagataa
    11641 attttcttgc ggagcgagag ctgcgtgccg cccgactcaa tcggccggtt gatgtcgggg
    11701 ctggaaccct gaaggttgag gcgcgggacg acgttgacgc cctcgaccag aattttgagc
    11761 gtcggcgggt agtggtcgag gttgctggtg tcgaagatcg tgcggtcgct gatggccacc
    11821 ttgctgcggg ggtcaaggta gagctgc
//
LOCUS       DASVES010000532         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U2068
            k141_1294262, whole genome shotgun sequence.
ACCESSION   DASVES010000532 DASVES010000000
VERSION     DASVES010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961388
            Sequence Read Archive: SRR25158500
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 21:36:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,451
            Genes (coding)                    :: 4,429
            CDSs (with protein)               :: 4,429
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 1 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294262"
                     /isolate="SMAG_U2068"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.86305556 N 84.030165 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VF240_22640"
     CDS             <1..367
                     /locus_tag="VF240_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178073.1"
                     /translation="PILGNLFKSKSFQKNETELMFIVTAQIVKPVNRDDLPVMPNVEN
                     LKKGSPLGIEPKDDGIDGQSGFSIKSGETKPEADAPAVKAPAPAATNGPVATPAAGAS
                     VPVASAVLQPAPAKVSPNQ"
     gene            430..1605
                     /locus_tag="VF240_22645"
     CDS             430..1605
                     /locus_tag="VF240_22645"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX7178074.1"
                     /translation="MSNKQRDEMPRSERGTIIVMTAILMLGLFLAMGLAIDVSRVYAV
                     RAELQNAADAAALTAARELNGGIGGINDAVTRANAIANNYGFTRTPITVASVEFATAL
                     NGTYVNQATAAGNAANIKFVRVTTEAVDVNMLFAVTAFVDGTHAESRNAVAGESVGIN
                     GICDFFPIAVALTNPNPAANTTMTLNFTQGSGSSATLANQDYIILEVPDINGNGAPET
                     AVLSAGLTSICASMGANIEFKMTPSANLNNGPRQITDGVNTRFNVYANGYGNALQPAT
                     FPPDSNVRENISYADYKDRTSVTAPSPNAPGEDDRRILIAPIVLPGTYNPPRTQIVNW
                     GAFFMKKRSTVSNPCNQASAACGDIEVEWVDEGVMIGRGYYDPTGGSSSLTLPVLYK"
     gene            1619..2101
                     /locus_tag="VF240_22650"
     CDS             1619..2101
                     /locus_tag="VF240_22650"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX7178075.1"
                     /translation="MGKRVLVKSVVRRFGRREDGTQMIEFAIVLPILLLLFAGATEIG
                     RMFHTYTTLAKATRAGARYLTTVQSVSASTNAAKNVVMCGSAAGCGGQDQPELILSDL
                     TADQIEITPPVNGAAVKYVTVSITGYTYQPLVFDLNTMTGSSGVFNPDLSPSTTMRYK
                     "
     gene            2135..2578
                     /locus_tag="VF240_22655"
     CDS             2135..2578
                     /locus_tag="VF240_22655"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7178076.1"
                     /translation="MNRQQPHRAGERGATLLEFVITATVYFMMLIGIVAISHLYFTHN
                     ALVESTRRGARYATFNLTSTDAQIKEVVVYGMSPPAAGAQPLVVGLTTDDVEVTRTNF
                     GVAQGTVTVYIDGYQYNLVSPVISSLITMPEYRTTLPGESAGYMP"
     gene            2638..>3368
                     /locus_tag="VF240_22660"
     CDS             2638..>3368
                     /locus_tag="VF240_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7178077.1"
                     /translation="MQPLTFITLSPDREAFKELSDALARDEGTRLLADCSTPAELLGA
                     IPRLRPSAALIVFGSGNSEQALALVKKISAEFPQTAVITAARDASILLNSVRAGAREF
                     LLLPVRAEELATVLGRTREFSVAESNRQSARKTGRVVGVFSNKGGSGVSFLAANLAAS
                     MPVPTILADLNLQAGDAASFLNLEAKHTVADMVKNLVRLDDALVQSFITYHSGRLSLL
                     AAPREAHEAEDIHPENVTEVISHLR"
BASE COUNT          669 a         1171 c         1005 g          523 t
ORIGIN      
        1 ccccatcctc ggcaacctct tcaagtcgaa gtcgttccag aagaatgaga ccgagctgat
       61 gttcatcgtc acggcgcaga tcgtgaagcc ggtcaaccgc gacgacctgc ccgtgatgcc
      121 caacgtcgaa aacctgaaga aaggctcgcc gctcggcatc gagccgaagg atgacggaat
      181 cgacggccag agcgggttct ccatcaagtc gggcgaaacc aagcctgagg cggacgcccc
      241 ggccgtgaag gcccctgccc ccgccgccac aaacggcccc gtcgcgacgc ccgccgccgg
      301 cgccagcgtg ccggtcgcct cggcggtgct ccagcctgcg ccggcgaagg tttcgcccaa
      361 ccagtgaggt ggttcgtcga aaaagtagtt cttcgccgct aaccctcgcc gaggtgacca
      421 cggatgttca tgagcaataa acagagagat gagatgccgc gctccgagcg cggcaccatc
      481 atcgtgatga ccgccatcct gatgctcggc ctattcctcg cgatggggct ggccatcgac
      541 gtctcgcgcg tctacgccgt ccgcgcggaa ctccagaacg ccgccgacgc cgccgcgctc
      601 accgccgcgc gcgagctcaa cggcggcatc ggcggcatca acgatgccgt cacccgcgcc
      661 aacgcgatag ccaacaacta cggctttacg aggacgccca tcacggtggc gagcgtcgag
      721 ttcgccaccg ccctcaacgg tacttacgtc aaccaggcga ctgccgcagg aaacgccgcc
      781 aacatcaagt tcgtccgcgt caccacggag gccgtcgatg tcaacatgct gttcgcggtg
      841 acagcgttcg ttgacgggac gcacgccgaa tcgaggaacg ccgtcgcggg agaatcggtg
      901 ggcatcaacg gcatctgcga cttcttcccc atcgccgtcg cgctcaccaa ccccaacccg
      961 gccgcgaaca ccacgatgac gctcaacttc acgcagggct cgggcagttc cgcgacgctc
     1021 gccaaccagg actacatcat cctcgaggtg cccgacatca acggcaacgg cgcgcccgag
     1081 acggcggtgc tctccgccgg cctcacctcg atctgcgcgt cgatgggggc caatatcgaa
     1141 ttcaaaatga cgccgagcgc gaacctcaac aacgggccga gacagatcac cgacggcgtc
     1201 aacacgcgct tcaacgtcta cgccaacggc tacgggaacg cccttcaacc cgcgaccttc
     1261 cccccggaca gcaacgtccg ggagaacatc agctacgctg attacaagga caggacgagt
     1321 gtcacggccc ccagcccgaa cgcgccgggg gaggacgacc ggcgcatcct catcgccccc
     1381 atcgtcctgc cgggcaccta caacccgccg cgcacacaga tcgtcaactg gggggcgttc
     1441 ttcatgaaga aaaggtctac cgtttcgaac ccctgcaacc aggcgtcggc ggcctgcggg
     1501 gacatcgagg tcgagtgggt ggacgagggc gtgatgatcg ggcgcggcta ctacgatccg
     1561 accggcggct cttcgagtct cacgcttccg gtcctctaca aataagttga tgaagattat
     1621 gggcaaacgg gtgttggtca aaagtgtggt acggcgcttc ggccgtcgcg aggacggcac
     1681 gcagatgatc gagttcgcca tcgtgctgcc gatcctgctc ctgctcttcg ccggcgcgac
     1741 cgaaatcggg cgcatgttcc acacctacac gacgctggcc aaggcgacgc gcgccggggc
     1801 gcgctacctc accaccgtcc agagcgtcag cgcgagcacg aacgcggcca agaacgtcgt
     1861 catgtgcggg tccgcggcgg gctgcggcgg gcaggatcag ccggagttaa ttctttccga
     1921 cctgacggcg gatcagatcg agattacccc gcccgtcaac ggggccgccg tcaaatacgt
     1981 cacggtctcg atcaccggct acacctatca gccgctggtc ttcgacctga acacaatgac
     2041 gggcagcagc ggtgtcttca accccgacct gagtccgtcc acgacgatgc ggtacaagtg
     2101 aggatggact ggcgggctta cggaagttaa agagatgaac aggcaacagc cacacagggc
     2161 gggcgagcgc ggggcgacgc tgctagaatt cgtcatcacg gcgacggtct acttcatgat
     2221 gctcatcggc atcgtcgcca tctcgcatct ctacttcacg cacaacgcgc tcgtcgaatc
     2281 cactcggcgc ggcgcgcgct acgccacctt caacctgacg agcaccgacg cgcagatcaa
     2341 ggaagtggtc gtctacggca tgtccccgcc cgccgccggc gcccagccgc tggtggtcgg
     2401 gctgacgacc gacgatgtcg aggtcactcg cactaacttc ggtgtcgcgc agggcaccgt
     2461 caccgtctat atcgacggct atcagtacaa cctcgtgagt ccggtcatca gcagcctcat
     2521 cacgatgccg gagtaccgca cgacgctccc gggcgagagc gccggttaca tgccgtagca
     2581 agagttctgg caagagacga gcagggtgga tcacagagca aggggaaata actaatcatg
     2641 caaccgctaa cattcatcac gctgagcccc gaccgcgagg cgttcaaaga gctgtccgac
     2701 gcgctggccc gcgatgaggg cacgcgcctg ctggccgact gttccacccc ggcggagctg
     2761 ctgggagcga tcccacgtct gcgcccgtcg gccgccctca ttgtgttcgg cagcgggaac
     2821 tcggagcagg cgctggcgct cgtcaaaaaa atctccgcgg aattcccgca aacggccgtc
     2881 atcacggcgg cccgcgacgc ctcgattctc ctcaacagcg tgcgcgcggg cgcgcgcgaa
     2941 ttcctcctcc tgcctgtcag ggcggaagag ctggccaccg tgctgggccg aacccgggag
     3001 ttctcggtcg ccgagagcaa ccgccagtcg gcgcgcaaga cgggccgcgt cgtcggcgtc
     3061 ttctcgaaca agggcggctc gggcgtctcc ttcctcgccg ccaacctcgc cgcgtcgatg
     3121 cccgtcccga ccatcctcgc cgacctcaac ctccaggcgg gcgacgccgc gtcgtttctc
     3181 aatctggagg cgaaacacac tgtcgccgac atggtgaaaa atctcgtgcg cctcgacgac
     3241 gcgctcgtcc agagcttcat cacgtaccac tccggccggc tgtcgctgct cgccgccccg
     3301 cgcgaggcgc atgaggccga agacattcac cccgaaaatg tgacggaggt catcagtcat
     3361 ctgcgcca
//
